ENGINEERED GRAM-NEGATIVE ENDOLYSINS

Abstract

The present invention relates in general to the field of antimicrobial enzymes. In particular, the present invention relates to a polypeptide comprising the amino acid sequence of a globular Gram-negative endolysin and the amino acid sequence of a cell wall binding domain of i) a modular Gram-negative endolysin or ii) a bacteriophage tail/baseplate protein. The present invention relates also to corresponding nucleic acids, vectors, bacteriophages, host cells, and compositions. The present inventions also relates to the use of said polypeptides, nucleic acids, vectors, bacteriophages, host cells, and compositions in methods for treatment of the human or animal body by surgery or therapy or in diagnostic methods practiced on the human or animal body. The polypeptides, nucleic acids, vectors, bacteriophages, host cells, and compositions according to the invention may also be used as an antimicrobial in, e.g., food or feed, in cosmetics, or as disinfecting agent.

Description

[0001] This application is a continuation of U.S. patent application Ser. No. 16/500,163, filed Oct. 2, 2019, as a national phase application under 35 U.S.C. .sctn. 371 of International Application No. PCT/IB2018/052253, filed Apr. 2, 2018, which claims benefit of priority to International Application No. PCT/IB2017/051886, filed Apr. 3, 2017, the entire contents of each of which are hereby incorporated by reference.

BACKGROUND OF THE INVENTION

I. Field of the Invention

[0002] The present invention relates to the field of antimicrobial enzymes. In particular, the present invention relates to a polypeptide comprising the amino acid sequence of a globular Gram-negative endolysin and the amino acid sequence of a cell wall binding domain of i) a modular Gram-negative endolysin or ii) a bacteriophage tail/baseplate protein. The present invention relates also to corresponding nucleic acids, vectors, bacteriophages, host cells, and compositions. The present inventions also relates to the use of said polypeptides, nucleic acids, vectors, bacteriophages, host cells, and compositions in methods for treatment of the human or animal body by surgery or therapy or in diagnostic methods practiced on the human or animal body. The polypeptides, nucleic acids, vectors, bacteriophages, host cells, and compositions according to the invention may also be used as an antimicrobial in, e.g., food or feed, in cosmetics, or as disinfecting agent.

II. Description of Related Art

[0003] Endolysins are peptidoglycan hydrolases encoded by bacteriophages (i.e. bacterial viruses). They are synthesized during late gene expression in the lytic cycle of phage multiplication and mediate the release of progeny virions from infected cells through degradation of the bacterial peptidoglycan. In terms of enzymatic activity they are usually either (1,4)-glycosylases (lysozymes), transglycosylases, amidases or endopeptidases. Antimicrobial application of endolysins was already suggested in 1991 by Gasson (GB2243611). Although the killing capacity of endolysins has been known for a long time, the use of these enzymes as antibacterials was ignored due to the success and dominance of antibiotics. Only after the appearance of multiple antibiotic resistant bacteria this simple concept of combating human pathogens with endolysins received interest. A compelling need to develop totally new classes of antibacterial agents emerged and endolysins used as `enzybiotics`--a hybrid term of `enzymes` and `antibiotics`--perfectly met this need. In 2001, Fischetti and coworkers demonstrated for the first time the therapeutic potential of bacteriophage Cl endolysin towards group A streptococci (Nelson et al., 2001). Since then many publications have established endolysins as an attractive and complementary alternative to control bacterial infections, particularly by Gram positive bacteria. Subsequently different endolysins against other Gram positive pathogens such as Streptococcus pneumoniae (Loeffler et al., 2001), Bacillus anthracis (Schuch et al., 2002), S. agalactiae (Cheng et al., 2005) and Staphylococcus aureus (Rashel et al, 2007) have proven their efficacy as enzybiotics. Nowadays, the most important challenge of endolysin therapy lies in the insensitivity of Gram-negative bacteria towards the exogenous action of endolysins, since the outer membrane shields the access of endolysins from the peptidoglycan.

[0004] Gram-negative bacteria possess an outer membrane, with its characteristic asymmetric bilayer as a hallmark. The outer membrane bilayer consists of an inner monolayer containing phospholipids (primarily phosphatidyl ethanolamine) and an outer monolayer that is mainly composed of a single glycolipid, lipopolysaccharide (LPS). There is an immense diversity of LPS structures in the bacterial kingdom and the LPS structure may be modified in response to prevailing environmental conditions. The stability of the LPS layer and interaction between different LPS molecules is mainly achieved by the electrostatic interaction of divalent ions (Mg2+, Ca2+) with the anionic components of the LPS molecule (phosphate groups in the lipid A and the inner core and carboxyl groups of KDO). Furthermore, the dense and ordered packing of the hydrophobic moiety of lipid A, favored by the absence of unsaturated fatty acids, forms a rigid structure with high viscosity. This makes it less permeable for lipophilic molecules and confers additional stability to the outer membrane (OM).

[0005] In contrast to Gram-negative bacteria, Gram-positive bacteria do not possess an outer membrane. The cytoplasmic membrane is surrounded by an up to 25 nm thick layer of peptidoglycan (which is only up to 5 nm for Gram-negative bacteria) which forms the cell wall. Main purpose of the cell wall of Gram-positives is to maintain bacterial shape and to counteract the internal bacterial cell pressure. Peptidoglycan, or murein, is a polymer consisting of sugars and amino acids. The sugar component consists of alternating residues of .beta.-(1,4) linked N-acetylglucosamine and N-acetylmuramic acid residues compose the sugar components. A peptide chain of three to five amino acids is attached to the N-acetylmuramic acid. The peptide chain can be cross-linked to the peptide chain of another strand forming a 3D mesh-like layer. The peptide chain may contain D- and L-amino acid residues and the composition may vary for different bacteria.

[0006] When comparing endolysins originating from phages infecting Gram-positive bacterial species versus those originating from phages infecting Gram-negative bacterial species, a striking difference can be observed, namely the general structure of the endolysins themselves. Phage endolysins of phages infecting Gram-positive bacteria are modular and comprise different individual functional domains or modules. The most common architecture is an N-terminal catalytic domain and a C-terminal cell wall-binding domain (Loessner, 2005). Some Gram-positive endolysins consist of three modules: an N-terminal and central catalytic domain with different specificity and a C-terminal substrate-binding module (Navarre et al., 1999; Pritchard et al., 2004; Yokoi et al., 2005). In contrast, endolysins encoded by phages associated with Gram-negative host cells are typically non-modular but single-module, globular proteins, with only a small number of exceptions. For more information on endolysins as antimicrobials see Nelson et al. ("Endolysins as Antimicrobials", Advances in Virus Research, Volume 83 (2012), p. 299-365, Eds. M. obocka and W. Szybalski, Elsevier).

[0007] Meanwhile, new strategies have emerged to utilize also endolysins originating from phages infecting Gram-negative bacterial species to control infections caused by Gram-negative bacteria. For this purpose, endolysins of Gram negative bacteria are fused with, e.g. cationic, amphipathic, hydrophobic or antimicrobial peptides. This type of fusion protein allows overcoming previous problems with the outer membrane of Gram-negative bacteria.

[0008] However, despite the advances in the art regarding antibacterial agents, there is still a need in the art for further improvement in the design of such antibacterial agents, in particular due to the increasing resistance to conventional antibiotics.

[0009] This problem is solved by the subject-matter as set forth below and in the appended claims.

SUMMARY OF THE INVENTION

[0010] The inventors of the present invention have surprisingly found that fusing globular Gram-negative endolysins with cell wall binding domains of Gram-negative endolysins or of bacteriophage tail/baseplate proteins yields more effective Gram-negative muralytic enzymes.

[0011] Thus, in a first aspect the present invention relates to a polypeptide comprising the amino acid sequence of a Gram-negative globular endolysin and the amino acid sequence of a cell wall binding domain of i) a Gram-negative modular endolysin or ii) a bacteriophage tail/baseplate protein.

[0012] Such inventive polypeptide may degrade, usually depending on the components chosen, the cell wall of bacteria selected from the group consisting of Acinetobacter, Aeromonas, Aggregatibacter, Azospirillum, Bacteroides, Burkholderia, Campylobacter, Candidatus, Caulobacter, Clavibacter, Cronobacter, Delftia, Enterobacter, Erwinia, Escherichia, Flavobacterium, Haemophilus, Iodobacteria, Klebsiella, Kluyvera, Mannheimia, Morganella, Neisseria, Pantoea, Pasteurella, Planktothrix, Pseudoalteromonas, Pseudomonas, Ralstonia, Salmonella, Shigella, Sinorhizobium, Sodalis, Synechococcus, Thalassomonas, Thermus, Vibrio, Xanthomonas, Xylella, and Yersinia.

[0013] The inventive polypeptide may additionally comprise at least one amino acid sequence selected from the group consisting of amphiphatic peptides, cationic peptides, hydrophobic peptides, naturally occurring antimicrobial peptides, sushi peptides and defensins. Such further peptide can enhance the antibacterial activity of the inventive polypeptide.

[0014] In further aspects, the present invention relates to nucleic acids encoding an inventive polypeptide, vectors or bacteriophages comprising an inventive nucleic acid as well as host cells comprising an inventive polypeptide, nucleic acid, vector, and/or bacteriophage.

[0015] The present invention relates in a further aspect also to compositions comprising a polypeptide, nucleic acid, vector, bacteriophage, and/or host cell according to the present invention. Such compositions are preferably pharmaceutical compositions comprising a pharmaceutically acceptable carrier, diluent, or excipient.

[0016] Finally, the present invention relates to polypeptides, nucleic acids, vectors, bacteriophages, host cells, compositions and/or kits of the present invention for use in methods of treatment, in particular for the treatment or prevention of bacterial infections.

DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS

I. Definitions

[0017] The term "polypeptide" as used herein refers in particular to a polymer of amino acid residues linked by peptide bonds in a specific sequence. The amino acid residues of a polypeptide may be modified by e.g. covalent attachments of various groups such as carbohydrates and phosphate. Other substances may be more loosely associated with the polypeptide, such as heme or lipid, giving rise to conjugated polypeptides which are also comprised by the term "polypeptide" as used herein. The term as used herein is intended to encompass also proteins. Thus, the term "polypeptide" also encompasses for example complexes of two or more amino acid polymer chains. The term "polypeptide" does encompass embodiments of polypeptides which exhibit optionally modifications typically used in the art, e.g. biotinylation, acetylation, pegylation, chemical changes of the amino-, SH- or carboxyl-groups (e.g. protecting groups) etc. As will become apparent from the description below, the polypeptide according to the present invention are fusion proteins, i.e. represent the linkage of at least two amino acid sequences which do not occur in this combination in nature. The term "polypeptide" as used herein is not limited to a specific length of the amino acid polymer chain, but typically the polypeptide will exhibit a length of more than about 150 amino acids. Usually, but not necessarily, a typical polypeptide of the present invention will not exceed about 1000 amino acids in length. The inventive polypeptide may for instance be at most about 750 amino acids long, at most about 500 amino acids long or at most about 300 amino acids long. A possible length range for the inventive polypeptide, without being limited thereto, may thus for example be about 200 to about 750 amino acids, or about 250 to about 600 amino acids. A particularly preferred range is about 250 to about 300 amino acids.

[0018] The term "fragment" as used herein refers to an amino acid sequence which is N-terminally, C-terminally, and/or on both termini truncated with respect to the respective reference sequence, for example a given endolysin or SEQ ID NO. Thus, a fragment of an amino acid sequence as used herein is an amino acid sequence which is at least one amino acid shorter than the respective reference sequence. A fragment of an amino acid sequence as used herein is preferably an amino acid sequence which is at most 20, more preferably at most 19, more preferably at most 18, more preferably at most 17, more preferably at most 16, more preferably at most 15, more preferably at most 14, more preferably at most 13, more preferably at most 12, more preferably at most 11, more preferably at most 10, more preferably at most 9, more preferably at most 8, more preferably at most 7, more preferably at most 6, more preferably at most 5, more preferably at most 4, more preferably at most 3, more preferably at most 2, most preferably 1 amino acid residue shorter than the respective reference amino acid sequence. The fragment may for example exhibit vis-a-vis the reference sequence a truncation of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 amino acids at the N-terminus, the C-terminus or both. It is understood that a polypeptide comprising a fragment of a given amino acid sequence does not comprise the full length of said reference amino acid sequence.

[0019] The term "endolysin" is generally understood be a person skilled in the art. As already mentioned previously, the term refers to a particular type of enzymes encoded in nature by bacteriophages. The bacteriophage utilizes such enzyme to release viral progeny from the inner of the infected bacterial cell. The enzyme hydrolyzes the peptidoglycan in the cell wall, leading to rupture of the bacterial cell. While all endolysins are peptidoglycan degrading enzymes, the actual reaction catalyzed, i.e. the actual bond cleaved in the peptidoglycan of bacteria, may be different. In terms of reaction catalyzed, endolysins may be for example glycosidases, amidases, endopeptidases, or lytic transglycosylases. As will be known by a person skilled in the art, the term "endolysin" does not encompass any enzymes catalyzing the same reactions, but which are not derived from bacteriophages. While such enzymes (e.g., hen egg-white lysozyme) catalyze formally the same reaction, they differ significantly from endolysins, e.g. in terms of biological function, evolutionary background and structure. The term "endolysin", as used herein encompasses naturally occurring endolysins, enzymatically active truncated versions thereof as well as technically modified endolysins deriving from these (i.e. naturally occurring endolysins and their enzymatically active fragments), e.g. with increased heat stability, reduced aggregation etc. Such modified endolysins will usually exhibit a sequence identity of at least 80%, preferably at least 82.5%, more preferably at least 85%, more preferably at least 87.5%, more preferably at least 90%, more preferably at least 92.5%, more preferably at least 95%, more preferably at least 97.5%, or most preferably at least 99% or more with the respective naturally occurring endolysin or its enzymatically active fragment. The term "Gram negative endolysin" refers to endolysins deriving from bacteriophages targeting Gram negative bacteria.

[0020] A "modular" endolysin, as used herein, is an endolysin which exhibits at least two distinct functional domains, namely at least one "enzymatically active domain" (EAD) and at least one "cell-wall-binding domain" (CBD). While the former provides the actual enzymatic activity, the latter may provide for target binding. Due to their domain character, these two activities can be separated from each other. Endolysins lacking a distinct CBD do not fall under the term "modular endolysin".

[0021] A "cell wall binding domain", or CBD, is an amino acid sequence within an endolysin sequence or phage tail/baseplate protein which folds into a structurally discreet module. The role of a CBD is to bind to the peptidoglycan and direct the catalytic machinery of the full length endolysin or phage tail/baseplate protein onto its substrate, thus enhancing the catalytic efficiency of the multimodular peptidoglycan-degrading enzyme. CBDs are themselves devoid of any catalytic activity.

[0022] An "enzymatically active domain" (EAD), as used herein, refers to an amino acid sequence within a modular endolysin sequence which folds into a structurally discreet module. An EAD exerts a catalytic, enzymatic function, i.e. may act for example as endopeptidase, chitinase, T4 like muraminidase, lambda like muraminidase, N-acetyl-muramoyl-L-alanine-amidase (amidase), muramoyl-L-alanine-amidase, muramidase, lytic transglycosylase (C), lytic transglycosylase (M), N-acetyl-muramidase (lysozyme), N-acetyl-glucosaminidase or transglycosylase. Since EADs are derived from modular endolysins, an EAD will exhibit a high degree of sequence identity with a modular endolysin, i.e. will exhibit a sequence identity of at least 95%, more preferably at least 97.5%, or most preferably even 100% with a naturally occurring modular endolysin or its enzymatically active fragment.

[0023] As used herein, "globular endolysins" are those endolysins lacking the modular organization and structure of an EAD and a CBD. The term is not intended to encompass fragments of modular endolysins which only retain the enzymatic active domain of a modular endolysin, i.e. an EAD is not a globular endolysin. Hence, a globular endolysin will not exhibit any significant sequence identity with a naturally occurring modular endolysin or its enzymatically active fragment. Usually, a globular endolysin will exhibit less than 90%, more preferably less than 85%, more preferably less than 80%, more preferably less than 75%, more preferably less than 70%, more preferably less than 60%, and most preferably less than 50% or less sequence identity with modular endolysins.

[0024] The term "bacteriophage tail/baseplate protein" is generally understood be a person skilled in the art. Tail proteins and baseplate proteins are proteins of bacteriophages. Binding structures located in the tail fiber and/or baseplate of bacteriophages play an important role in mediating injection of the phage genome into the host cell. Tail fiber proteins are positioned at the tip of the tail and are responsible for binding to the cell surface by recognizing a potential host bacterium and attaching to its outer surface. Baseplate proteins control the transfer of the genetic material and can have also cell binding properties. Especially for Myoviruses of Gram negative bacteria (e.g. T4 or P2 phages) different motifs are described which show homology to peptidoglycan binding domains like LysM. Another example is the gp5 of the ICP1 vibrio phage and related proteins encoded in the genome of phages infecting different species like e.g. Methylobacter sp. These consist of a peptidoglycan binding domain and an enzymatic active domain, able to degrade the murein layer of the host bacteria.

[0025] The term "% sequence identity" is generally understood in the art. Two sequences to be compared are aligned to give a maximum correlation between the sequences. This may include inserting "gaps" in either one or both sequences, to enhance the degree of alignment. A % identity may then be determined over the whole length of each of the sequences being compared (so-called global alignment), that is particularly suitable for sequences of the same or similar length, or over shorter, defined lengths (so-called local alignment), that is more suitable for sequences of unequal length. In the above context, an amino acid sequence having a "sequence identity" of at least, for example, 95% to a query amino acid sequence, is intended to mean that the sequence of the subject amino acid sequence is identical to the query sequence except that the subject amino acid sequence may include up to five amino acid alterations per each 100 amino acids of the query amino acid sequence. In other words, to obtain an amino acid sequence having a sequence of at least 95% identity to a query amino acid sequence, up to 5% (5 of 100) of the amino acid residues in the subject sequence may be inserted or substituted with another amino acid or deleted. Methods for comparing the identity and homology of two or more sequences are well known in the art. The percentage to which two sequences are identical can for example be determined by using a mathematical algorithm. A preferred, but not limiting, example of a mathematical algorithm which can be used is the algorithm of Karlin et al. (1993), PNAS USA, 90:5873-5877. Such an algorithm is integrated in the BLAST family of programs, e.g. BLAST or NBLAST program (see also Altschul et al., 1990, J. Mol. Biol. 215, 403-410 or Altschul et al. (1997), Nucleic Acids Res, 25:3389-3402), accessible through the home page of the NCBI at world wide web site ncbi.nlm.nih.gov) and FASTA (Pearson (1990), Methods Enzymol. 83, 63-98; Pearson and Lipman (1988), Proc. Natl. Acad. Sci. U. S. A 85, 2444-2448). Sequences which are identical to other sequences to a certain extent can be identified by these programs. Furthermore, programs available in the Wisconsin Sequence Analysis Package, version 9.1 (Devereux et al, 1984, Nucleic Acids Res., 387-395), for example the programs BESTFIT and GAP, may be used to determine the % identity between two polypeptide sequences. BESTFIT uses the "local homology" algorithm of (Smith and Waterman (1981), J. Mol. Biol. 147, 195-197) and finds the best single region of similarity between two sequences. If herein reference is made to an amino acid sequence sharing a particular extent of sequence identity to a reference sequence, then said difference in sequence is preferably due to conservative amino acid substitutions. Preferably, such sequence retains the activity of the reference sequence, e.g. retains the activity of degrading the peptidoglycan layer of Gram-negative bacteria, albeit maybe at a slower rate. In addition, if reference is made herein to a sequence sharing "at least" at certain percentage of sequence identity, then 100% sequence identity are preferably not encompassed.

[0026] As used herein, the term "cationic peptide" refers preferably to a peptide having positively charged amino acid residues. Preferably a cationic peptide has a pKa-value of 9.0 or greater. Typically, at least four of the amino acid residues of the cationic peptide can be positively charged, for example, lysine or arginine. "Positively charged" refers to the side chains of the amino acid residues which have a net positive charge at about physiological conditions. The term "cationic peptide" as used herein refers also to polycationic peptides, but also includes cationic peptides which comprise for example less than 20%, preferably less than 10% positively charged amino acid residues.

[0027] The term "polycationic peptide", as used herein, refers preferably to a peptide composed of mostly positively charged amino acid residues, in particular lysine and/or arginine residues. A peptide is composed of mostly positively charged amino acid residues if at least about 20, 30, 40, 50, 60, 70, 75, 80, 85, 90, 95 or about 100% of the amino acid residues are positively charged amino acid residues, in particular lysine and/or arginine residues. The amino acid residues being not positively charged amino acid residues can be neutrally charged amino acid residues and/or negatively charged amino acid residues and/or hydrophobic amino acid residues. Preferably the amino acid residues being not positively charged amino acid residues are neutrally charged amino acid residues, in particular serine and/or glycine.

[0028] The term, "antimicrobial peptide" (AMP), as used herein, refers preferably to any naturally occurring peptide that has microbicidal and/or microbistatic activity on for example bacteria, viruses, fungi, yeasts, mycoplasma and protozoa. Thus, the term "antimicrobial peptide" as used herein refers in particular to any peptide having anti-bacterial, anti-fungal, anti-mycotic, anti-parasitic, anti-protozoal, anti-viral, anti-infectious, anti-infective and/or germicidal, algicidal, amoebicidal, microbicidal, bactericidal, fungicidal, parasiticidal, protozoacidal, protozoicidal properties. Preferred are anti-bacterial peptides. The antimicrobial peptide may be a member of the RNase A super family, a defensin, cathelicidin, granulysin, histatin, psoriasin, dermicidine or hepcidin. The antimicrobial peptide may be naturally occurring in insects, fish, plants, arachnids, vertebrates or mammals. Preferably the antimicrobial peptide may be naturally occurring in insects, fish, plants, arachnids, vertebrates or mammals. Preferably the antimicrobial peptide may be naturally occurring in radish, silk moth, wolf spider, frog, preferably in Xenopus laevis, Rana frogs, more preferably in Rana catesbeiana, toad, preferably Asian toad Bufo bufo gargarizans, fly, preferably in Drosophila, more preferably in Drosophila melanogaster, in Aedes aegypti, in honey bee, bumblebee, preferably in Bombus pascuorum, flesh fly, preferably in Sarcophaga peregrine, scorpion, horseshoe crab, catfish, preferably in Parasilurus asotus, cow, pig, sheep, porcine, bovine, monkey and human. As used herein, an "antimicrobial peptide" (AMP) may in particular be a peptide which is not a cationic peptide, polycationic peptide, amphipathic peptide, sushi peptide, defensins, and hydrophobic peptide, but nevertheless exhibits antimicrobial activity.

[0029] The term "sushi peptide", as used herein, refers to complement control proteins (CCP) having short consensus repeats. The sushi module of sushi peptides functions as a protein-protein interaction domain in many different proteins. Peptides containing a Sushi domain have been shown to have antimicrobial activities. Preferably, sushi peptides are naturally occurring peptides.

[0030] The term "amphipathic peptide", as used herein, refers to synthetic peptides having both hydrophilic and hydrophobic functional groups. Preferably, the term "amphipathic peptide" as used herein refers to a peptide having a defined arrangement of hydrophilic and hydrophobic groups e.g. amphipathic peptides may be e.g. alpha helical, having predominantly non polar side chains along one side of the helix and polar residues along the rest of its surface.

[0031] The term "hydrophobic group", as used herein, refers preferably to chemical groups such as amino acid side chains which are substantially water insoluble, but soluble in an oil phase, with the solubility in the oil phase being higher than that in water or in an aqueous phase. In water, amino acid residues having a hydrophobic side chain interact with one another to generate a non-aqueous environment. Examples of amino acid residues with hydrophobic side chains are valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, and proline residues.

[0032] The term "hydrophobic peptide", as used herein, refers to a hydrophobic peptide, which is preferably composed of mostly amino acid residues with hydrophobic groups. Such peptide is preferably composed of mostly hydrophobic amino acid residues, i.e. at least about 20, 30, 40, 50, 60, 70, 75, 80, 85, 90, 95 or at least about 100% of the amino acid residues are hydrophobic amino acid residues. The amino acid residues being not hydrophobic are preferably neutral and preferably not hydrophilic.

[0033] The term "comprising", as used herein, shall not be construed as being limited to the meaning "consisting of" (i.e. excluding the presence of additional other matter). Rather, "comprising" implies that optionally additional matter may be present. The term "comprising" encompasses as particularly envisioned embodiments falling within its scope "consisting of" (i.e. excluding the presence of additional other matter) and "comprising but not consisting of" (i.e. requiring the presence of additional other matter), with the former being more preferred.

[0034] The use of the word "a" or "an", when used herein, may mean "one," but it is also consistent with the meaning of "one or more," "at least one," and "one or more than one."

II. Polypeptides

[0035] As already mentioned, the present invention relates in a first aspect to a polypeptide comprising the amino acid sequence of a Gram-negative globular endolysin and the amino acid sequence of a cell wall binding domain of i) a Gram-negative modular endolysin or ii) a bacteriophage tail/baseplate protein.

[0036] Endolysins are well known to a person skilled in the art of antimicrobial agents. Many of these proteins have been sequenced and their structure and domain architecture (including CBDs and EADs) analyzed. Usually it is thus very simple to elucidate the presence of a cell wall binding domain in a given endolysin sequence, for example on basis of homology analyses. In case of doubt, whether a given sequence of an endolysin acts as cell wall binding domain or not, said property can also be analyzed by routine test known in the art. Exemplary tests are provided for instance in Mol Microbiol. 2002 April; 44(2):335-49 and Briers et al. (Mol Microbiol. 2007 September; 65(5):1334-44). Briefly, the candidate cell wall-binding domain is fused (e.g. N-terminally) to green fluorescent protein (GFP). Subsequently, the GFP-fusion protein is incubated with the target bacteria of the parent endolysin. If these are gram-negative bacteria, then the outer membrane is permeabilized in advance by treatment with a chloroform-saturated buffer (chloroform-saturated 0.05 M Tris-buffer (pH 7.7), 45 min (Lavigne et al., Cell Mol Life Sci. 2004 November; 61(21):2753-9). The candidate CBD-GFP fusion is then added to the permeabilized cells (e.g. final concentration 5 .mu.M). Purified recombinant GFP is used in the negative control. This mixture is then incubated, e.g. for 5 min at 25.degree. C., subsequently spun down and the supernatant discarded. The cell pellet may then be washed (e.g. twice in the corresponding buffer) and analyzed for GFP-binding via suitable means such as epifluorescence microscopy, flow cytometry or confocal fluorescence microscopy. In addition, a specific peptidoglycan binding test can be performed Briers et al. (Mol Microbiol. 2007 September; 65(5):1334-44). For this purpose murein of the target bacteria is isolated and contacted with the candidate CBD-GFP fusion protein and binding is once again analyzed, e.g. via epifluorescence microscopy.

[0037] Exemplary cell wall binding domains of Gram-negative endolysins, which may be used in carrying out the present invention, are cell wall binding domains deriving from the endolysins of bacteriophages .PHI.KZ and EL, or from the endolysins OBPgpLYS, PVPSE1gp146, and 201.phi.2-1.

[0038] Phage baseplate/tail proteins are also known in the art and information on sequence, structure and domain architecture is frequently available. Otherwise, similar tests as mentioned above for endolysins are suited to identify cell wall binding domains of bacteriophage tail/baseplate proteins. An exemplary cell wall binding domain of bacteriophage tail/baseplate protein, which may be used in carrying out the present invention, is the cell wall binding domains deriving from the baseplate protein of Vibrio phage ICP1 (see YP_004251150.1) or Vibrio phage RYC (BAV80844.1).

[0039] Examples for specific sequences comprising cell wall binding domains (deriving from Gram-negative endolysins or bacteriophage tail/baseplate proteins) are provided herein in SEQ ID Nos: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, and 17, and sequences having at least 80% sequence identity to any of these while retaining the property of cell wall binding. Particularly preferred sequences are SEQ ID Nos: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 and 14, and sequences having at least 80% sequence identity to any of these while retaining the property of cell wall binding. Most preferred are sequences deriving from the cell wall binding domain of KZ144 endolysin, such as SEQ ID Nos: 1, 2, 3, 4, 5, 6, 7, and 8, and sequences having at least 80% sequence identity to any of these while retaining the property of cell wall binding.

[0040] As previously mentioned, the inventive polypeptide comprises aside of the amino acid sequence of a cell wall binding domain of a Gram-negative modular endolysin or a bacteriophage baseplate tail protein also the amino acid sequence of a Gram-negative globular endolysin. A person skilled in the art will be readily able to ascertain whether a given polypeptide derives from a Gram-negative endolysin exhibiting a cell wall binding domain (i.e. is a modular endolysin) or derives from a Gram-negative endolysin which does not exhibit such domain structure (globular endolysin). Examples for globular endolysins are for example provided in Table 1 below.

TABLE-US-00001 TABLE 1 Examples for globular endolysins Host Phage name Protein ID Acinetobacter Acinetobacter phage Ac42 YP_004009379.1 Acinetobacter Acinetobacter phage Acj61 YP_004009630.1 Acinetobacter Acinetobacter phage Acj9 YP_004010153.1 Aeromonas Aeromonas phage PX29 ADQ53036.1 Aeromonas Aeromonas phage PAS-1 AEM36042.1 Aeromonas Aeromonas phage phiAS7 AEZ65050.1 Aeromonas Aeromonas phage phiAS4 YP_003969055.1 Aeromonas Aeromonas phage 44RR2.8t NP_932578.1 Aeromonas Aeromonas phage 25 YP_656449.1 Aeromonas Aeromonas phage 31 YP_238949.1 Aeromonas Aeromonas phage Aeh1 NP_944217.1 Aeromonas Aeromonas phage phiO18P YP_001285657.1 Aeromonas Aeromonas phage 65 YP_004300997.1 Aeromonas Aeromonas phage phiAS5 YP_003969406.1 Azospirillum Azospirillum phage Cd YP_001686894.1 Aggregatibacter Aggregatibacter phage S1249 YP_003344813.1 Bacteroides Bacteroides phage B40-8 YP_002221548.1 Bacteroides Bacteroides phage B124-14 YP_005102482.1 Burkholderia Burkholderia phage BcepF1 YP_001039778.1 Burkholderia Burkholderia phage BcepB1A YP_024909.1 Burkholderia Burkholderia phage Bcep22 YP_001531197.1 Burkholderia Burkholderia phage BcepNazgul NP_918971.2 Burkholderia Burkholderia phage phiE255 YP_001111252.1 Burkholderia Burkholderia phage KS9 YP_003090199.1 Burkholderia Burkholderia phage phi1026b NP_945054.1 Burkholderia Burkholderia phage phiE125 NP_536381.1 Burkholderia Burkholderia phage BcepC6B YP_024942.1 Burkholderia Burkholderia phage Bcep176 YP_355393.1 Burkholderia Burkholderia phage BcepIL02 YP_002922746.1 Burkholderia Burkholderia phage BcepMu YP_024695.1 Burkholderia Burkholderia phage KS10 YP_002221425.1 Burkholderia Burkholderia phage phi644-2 YP_001111104.1 Campylobacter Campylobacter phage CP220 CBJ93929.1 Campylobacter Campylobacter phage CPt10 CBJ94327.1 Candidatus Acyrthosiphon pisum bacteriophage APSE-1 NP_050974.1 Candidatus Bacteriophage APSE-2 ACJ10174.1 Candidatus Bacteriophage APSE-4 ACJ10096.1 Candidatus Bacteriophage APSE-7 ACJ10111.1 Candidatus Bacteriophage APSE-5 ACJ10082.1 Candidatus Bacteriophage APSE-3 ACJ10123.1 Candidatus Bacteriophage APSE-6 ACJ10136.1 Caulobacter Caulobacter phage Cd1 ADD21680.1 Cronobacter Cronobacter phage ENT47670 ADZ13641.1 Cronobacter Cronobacter phage E5P2949-1 AEM24793.1 Cronobacter Cronobacter phage ES2 AEM24706.1 Cronobacter Cronobacter phage ENT39118 ADZ13601.1 Delftia Deftia phage phiW-14 YP_003358866.1 Enterobacter Enterobacteria phage F20 AEQ39188.1 Escherichia Escherichia phage TL-2011b AEW24559.1 Escherichia Escherichia phage vB_EcoM_ECO1230-10 ADE87938.1 Escherichia Escherichia phage HK639 YP_004934099.1 Escherichia Escherichia phage phiV10 YP_512283.1 Escherichia Escherichia phage rv5 YP_002003587.1 Escherichia Escherichia phage K1H ADA82342.1 Escherichia Escherichia phage K1ind3 ADA82488.1 Escherichia Escherichia phage K1G ADA82292.1 Escherichia Escherichia phage wV7 AEM00790.1 Escherichia Escherichia phage HK75 YP_004934160.1 Escherichia Stx2-converting phage 1717 YP_002274257.1 Escherichia Stx2-converting phage 86 YP_794054.1 Escherichia Stx2 converting phage II YP_003828995.1 Escherichia Escherichia phage phiEB49 AEI91208.1 Escherichia Enterobacteria phage Ti YP_003933.1 Escherichia Enterobacteria phage T3 NP_523313.1 Escherichia Enterobacteria phage T4 NP_049736.1 Escherichia Enterobacteria phage T5 YP_006868.1 Escherichia Erherobacteria phage TLS YP_001285558.1 Escherichia Enterobacteria phage vB_Ecom-VR7 YP_004063811.1 Escherichia Enterobacteria phage WV8 YP_002922821.1 Escherichia Enterobacteria phage Min27 ABY49900.1 Escherichia Enterobacteria phage lambda NP_040645.1 Escherichia Enterobacteria phage K1F YP_338105.1 Escherichia Enterobacteria phage IME10 AER08021.1 Escherichia Enterobacteria phage vB_EcoM-FV3 AEZ65218.1 Escherichia Escherichia phage TL-2011c AEW24625.1 Escherichia Enterobacteria phage Bp7 AEN93735.1 Escherichia Enterobacteria phage RB49 NP_891673.1 Escherichia Enterobacteria phage RTP YP_399008.1 Escherichia Enterobacteria phage BP-4795 YP_001449285.1 Escherichia Enterobacteria phage BA14 YP_002003466.1 Escherichia Enterobacteria phage 285P YP_004300550.1 Escherichia Enterobacteria phage P1 YP_006484.1 Escherichia Enterobacteria phage JS98 YP_001595245.1 Escherichia Enterobacteria phage AR1 BAI83135.1 Escherichia Enterobacteria phage SfV NP_599082.1 Escherichia Enterobacteria phage T7 NP_041973.1 Escherichia Enterobacteria phage phiEco32 YP_001671762.1 Escherichia Enterobacteria phage A5 ABF71471.1 Escherichia Enterobacteria phage 186 AAC34155.1 Escherichia Enterobacteria phage HK022 AAF30387.1 Escherichia Enterobacteria phage JS10 YP_002922463.1 Escherichia Enterobacteria phage P2 NP_046765.1 Escherichia Enterobacteria phage 933W (sensu lato) NP_049505.1 Escherichia Enterobacteria phage JK06 YP_277498.1 Escherichia Enterobacteria phage N15 NP_046950.1 Escherichia Enterobacteria phage K1E YP_425023.1 Escherichia Enterobacteria phage K1-5 YP_654144.1 Escherichia Enterobacteria phage JSE YP_002922178.1 Escherichia Enterobacteria phage IME08 YP_003734260.1 Escherichia Enterobacteria phage HK97 NP_037753.1 Escherichia Enterobacteria phage RB43 YP_239135.1 Escherichia Enterobacteria phage RB16 YP_003858447.1 Escherichia Enterobacteria phage phiP27 NP_543082.1 Escherichia Enterobacteria phage phiEcoM-GJ1 YP_001595416.1 Escherichia Enterobacteria phage Phil YP_001469446.1 Escherichia Enterobacteria phage EcoDS1 YP_002003756.1 Escherichia Enterobacteria phage cdtl YP_001272571.1 Escherichia Enterobacteria phage CC31 YP_004009990.1 Escherichia Enterobacteria phage K30 YP_004678738.1 Escherichia Enterobacteria phage CUS-3 ABQ88407.1 Escherichia Stxl converting phage BAC77971.1 Escherichia Stx2 converting phage I BAB88004.1 Escherichia Stxl-converting phage phi-O153 AAW21764.1 Escherichia Escherichia phage D108 YP_003335769.1 Escherichia Enterobacteria phage SPC35 YP_004306522.1 Escherichia Enterobacteria phage Mu NP_050626.1 Escherichia Enterobacteria phage RB69 NP_861818.1 Escherichia Enterobacteria phage RB14 YP_002854463.1 Escherichia Enterobacteria phage RB32 ABI94948.1 Escherichia Enterobacteria phage RB51 YP_002854084.1 Erwinia Erwinia phage phiEa116 CCA66256.1 Erwinia Erwinia phage vB_EamM-M7 AEJ81266.1 Erwinia Erwinia phage vB_EamM-Y2 AEJ81402.1 Erwinia Erwinia phage vB_EamP-L1 AEJ81484.1 Erwinia Erwinia phage phiEa21-4 YP_002456060.1 Erwinia Erwinia phage phiEa104 YP_004327012.1 Erwinia Erwinia phage Era103 YP_001039680.1 Flavobacterium Flavobacterium phage 11b YP_112524.1 Haemophilus Haemophilus phage SuMu AEG42272.1 Haemophilus Haemophilus phage HP1 NP_043495.1 Haemophilus Haemophilus phage HP2 NP_536831.1 Haemophilus Haemophilus phage Aaphi23 NP_852750.1 Iodobacteria Iodobacteriophage phiPLPE YP_002128449.1 Klebsiella Klebsiella phage K11 YP_002003804.1 Klebsiella Klebsiella phage KP32 YP_003347533.1 Klebsiella Klebsiella phage KP15 YP_003580002.1 Klebsiella Enterobacteria phage vB_KleM-RaK2 AFA44346.1 Kluyvera Kluyvera phage Kvp1 YP_002308397.1 Mannheimia Mannheimia phage phiMHa1l YP_655477.1 Mannheimia Mannheimia phage phiMhaA1-PHL101 ABD90561.1 Morganella Morganella phage MmP1 YP_002048642.1 Alphaproteobacteria Phage phiJL001 YP_224014.1 Pseudoalteromonas Pseudoalteromonas phage H105/1 YP_004327143.1 Pseudomonas Pseudomonas phage LKA1 YP_001522894.1 Pseudomonas Pseudomonas phage D3 NP_061527.1 Pseudomonas Pseudomonas phage F10 YP_001293405.1 Pseudomonas Pseudomonas phage F116 YP_164326.1 Pseudomonas Pseudomonas phage F8 YP_001294463.1 Pseudomonas Pseudomonas phage gh-1 NP_813758.1 Pseudomonas Pseudomonas phage LBL3 YP_002154189.1 Pseudomonas Pseudomonas phage LKD16 YP_001522837.1 Pseudomonas Pseudomonas phage LMA2 YP_002154280.1 Pseudomonas Pseudomonas phage LUZ19 YP_001671990.1 Pseudomonas Pseudomonas phage PA11 YP_001294626.1 Pseudomonas Pseudomonas phage PAJU2 YP_002284361.1 Pseudomonas Pseudomonas phage PaP3 NP_775256.1 Pseudomonas Pseudomonas phage PB1 YP_002455978.1 Pseudomonas Pseudomonas phage phi13 (S-segment) NP_690810.1 Pseudomonas Pseudomonas phage phi15 YP_004286199.1 Pseudomonas Pseudomonas phage phi-2 YP_003345505.1 Pseudomonas Pseudomonas phage philBB-PF7A YP_004306332.1 Pseudomonas Pseudomonas phage phikF77 YP_002727868.1 Pseudomonas Pseudomonas phage PT2 YP_002117830.1 Pseudomonas Pseudomonas phage PT5 YP_002117771.1 Pseudomonas Pseudomonas phage SN YP_002418854.1 Pseudomonas Pseudomonas phage phi297 YP_005098091.1 Pseudomonas Pseudomonas phage Bf7 YP_005098158.1 Pseudomonas Pseudomonas phage PaP1 AEK21612.1 Pseudomonas PseudomonasLUZ24 YP_001671940.1 Pseudomonas Pseudomonas phage phi-6 segment S NP_620343.1 Pseudomonas Pseudomonas phage vB_PaeS_PMG1 YP_005098234.1 Pseudomonas Enterobacteria phage phiKMV NP_877484.1 Pasteurella Pasteurella phage F108 YP_654740.1 Ralstonia Ralstonia phage RSB2 BAJ51815.1 Salmonella Salmonella phage SETP3 YP_001110823.1 Salmonella Bacteriophage PS3 CAA09701.1 Salmonella Erherobacteria phage ST104 YP_006397.1 Salmonella Enterobacteria phage SP6 AAR90036.1 Salmonella Enterobacteria phage Felix 01 NP_944846.1 Salmonella Enterobacteria phage P22 DAA01040.1 Salmonella Bacteriophage Wphi AAN28227.1 Salmonella Enterobacteria phage EPS7 YP_001836966.1 Salmonella Enterobacteria phage Fels-2 YP_001718740.1 Salmonella Salmonella phage ES18 YP_224214.1 Salmonella Salmonella phage PsP3 NP_958065.1 Salmonella Salmonella phage E1 YP_001742044.1 Salmonella Salmonella phage SETP12 ABN70688.1 Salmonella Salmonella phage SETP5 ABN70687.1 Salmonella Salmonella phage HK620 NP_112069.1 Salmonella Salmonella phage RE-2010 ADQ92398.1 Salmonella Phage Gifsy-1 YP_001700616.1 Salmonella Phage Gifsy-2 YP_001700672.1 Salmonella Salmonella phage c341 YP_003090277.1 Salmonella Salmonella phage epsilon15 NP_848233.1 Salmonella Salmonella phage epsilon34 YP_002533525.1 Salmonella Salmonella phage Fels-1 YP_001700560.1 Salmonella Salmonella phage phiSG-JL2 YP_001949762.1 Salmonella Salmonella phage SE1 YP_002455881.1 Salmonella Salmonella phage ST64B NP_700425.1 Salmonella Salmonella phage ST64T NP_720320.1 Salmonella Salmonella phage Vi06 YP_004306666.1 Salmonella Salmonella phage SPN1S YP_005098003.1 Salmonella Salmonella phage SE2 YP_005098118.1 Salmonella Salmonella phage SS3e YP_005097816.1 Salmonella Salmonella Phage PS34 O80288.1 Salmonella Enterobacteria phage 13a YP_002003950.1 Shigella Shigella phage Sf6 NP_958236.1 Shigella Shigella phage Shfl1 YP_004414884.1 Shigella Shigella phage Shfl2 YP_004415022.1 Shigella Shigella phage EP23 YP_004957490.1 Sinorhizobium Sinorhizobium phage PBC5 NP_542265.1 Sodalis Sodalis phage phiSG1 YP_516184.1 Sodalis Sodalis phage SO-1 YP_003344991.1 Synechococcus Synechococcus phage S-CBS2 YP_004421540.1 Synechococcus Synechococcus phage S-CRM01 YP_004508546.1 Synechococcus Synechococcus phage S-PM2 YP_195189.2 Synechococcus Synechococcus phage S-PM2 (another) YP_195188.1 Thalassomonas Thalassomonas phage BA3 YP_001552293.1 Thermus Thermus phage P23-45 YP_001467961.1 Thermus Thermus phage P23-77 YP_003169716.1 Thermus Thermus phage P23-77 (another) YP_003169717.1 Thermus Thermus phage P74-26 YP_001468077.1 Vibrio Vibrio phage ICP3 YP_004251275.1 Vibrio Vibrio phage K139 NP_536660.1 Vibrio Vibrio phage kappa YP_001650899.1 Vibrio Vibrio phage N4 YP_003347926.1 Vibrio Vibrio phage VP93 YP_002875665.1 Vibrio Vibrio phage ICP3_2009_B ADX87518.1 Vibrio Vibriophage VP4 YP_249586.1 Vibrio Vibrio phage ICP3_2007_A ADX87661.1 Xanthomonas Xanthomonas phage OP1 YP_453585.1 Xanthomonas Xanthomonas phage phiL7 YP_002922642.1 Xanthomonas Xanthomonas phage Xop411 YP_001285697.1 Xanthomonas Xanthomonas phage Xp10 NP_858975.1 Xylella Xylella phage Xfas53 YP_003344916.1 Yersinia Yersinia pestis phage phiA1122 NP_848277.1 Yersinia Yersinia phage Berlin YP_918995.1 Yersinia Yersinia phage L-413C NP_839858.1 Yersinia Yersinia phage phiR1-37 YP_004934318.1 Yersinia Yersinia phage phiYeO3-12 NP_052084.1 Yersinia Yersinia phage PY54 NP_892107.1 Yersinia Yersinia phage Yepe2 YP_002003326.1 Yersinia Yersinia phage PY100 CAJ28446.1 Yersinia Yersinia phage Yep-phi ADQ83168.1 Acinetobacter Acinetobacter phage vB_AbaP_CEB1 ALC76575.1

Salmonella Salmonella phage phi68 AHY18890.1 Salmonella Salmonella phage SPN9CC YP_006383882 Acinetobacter Acinetobacter phage vB_AbaP_Acibe1007 YP_009103259.1 Acinetobacter Acinetobacter phage Abp1 AFV51025.1 Pseudomonas Pseudomonas aeruginosa phage LUZ7 YP_003358335.1 Pseudomonas Pseudomonas aeruginosa phage LIT1 YP_003358446.1 Pseudomonas Pseudomonas phage vB_PaeP_C2-10_Ab09 YP_009031822.1 Pseudomonas Pseudomonas phage 2b.1b SEQ ID NO: 18 Salmonella Salmonella phage SBA-1781 AFU63467.1 Salmonella Salmonella phage Shivani AJA73488.1

[0041] The sequences of the globular endolysins of table 1 may be accessed for instance via the protein database of NCBI. It is understood that the sequences of the endolysins listed in table 1 may also be modified, e.g. may lack the N-terminal methionine to avoid a further start codon in the corresponding nucleic acid sequence. Using such marginally amended sequences is also within the scope of the present invention and it is understood, that when reference herein is made to endolysins of table 1, that also such modified endolysins are encompassed by said definition.

[0042] Particularly preferred sequences of globular endolysins, which may be used in carrying out the present invention, are sequences deriving from globular endolysins Abgp46, Lys68 of Salmonella phage phi68 and Lys394 endolysin. Exemplary sequences are provided as SEQ ID Nos. 18, 19, 20, 21, 22, 23, 24, 25, 26, 27 and 28.

[0043] The sequence of the cell wall binding domain (e.g. of the Gram-negative modular endolysin) and the sequence of the Gram-negative globular endolysin may be linked directly to each other or via an intermediate linker sequence, the linker sequence preferably not exceeding more than 50 amino acids in length, more preferably not more than 30 amino acids in length, even more preferably not more than 20 amino acids in length. Preferably, the cell wall binding domain is situated N-terminal of the sequence of the globular endolysin, i.e. of the domain with the enzymatic activity.

[0044] Non-limiting examples of polypeptides according to the present invention are provided in SEQ ID Nos. 29, 30, 31, 32, 33 and 34. If such sequence is to be combined with a further amino acid sequence stretch as defined further below, and said further amino acid sequence stretch is positioned N-terminal of the unit formed by the cell wall binding domain and globular endolysin sequences, said sequences are preferably used without methionine start codon (see SEQ ID Nos. 35, 36, 37, 38, 39 and 40)

[0045] A polypeptide according to the present invention exhibits preferably the activity of a peptidoglycan degrading enzyme, i.e. is capable of degrading bacterial peptidoglycan. Typically a polypeptide of the present invention will be capable of degrading the peptidoglycan of at least one type of Gram-negative bacteria, such as K. pneumoniae, E. coli or P. aeruginosa. The peptidoglycan degrading activity on gram negative bacteria can be measured by assays well known in the art, e.g. by muralytic assays in which the outer membrane of gram negative bacteria is permeabilized or removed (e.g. with chloroform) to allow the putative enzyme access to the peptidoglycan layer. If the enzyme is active, degradation of the peptidoglycan layer will lead to a drop of turbidity, which can be measured photometrically (see for example Briers et al., J. Biochem. Biophys Methods 70: 531-533, (2007).

[0046] A polypeptide according to the present invention may comprise additionally at least one further amino acid sequence stretch selected from the group consisting of amphipathic peptide, cationic peptide, polycationic peptide, hydrophobic peptide, or naturally occurring antimicrobial peptide, like sushi peptide and defensin. This additional at least one amino acid sequence stretch may in principle be present at any position in the inventive polypeptide, but is preferably present at the termini, i.e. in the N- or C-terminal region of the inventive polypeptide. Thus, this additional amino acid sequence stretch is preferably not positioned between the sequence of the cell wall binding domain (e.g. of the Gram-negative modular endolysin) and the sequence of the Gram-negative globular endolysin. Such additional amino acid sequence stretch may be fused directly, or via a peptide linker, to the rest of the polypeptide. It is understood that if one (or more) such additional amino acid sequence stretches according to the present invention are present in the N-terminal region of the inventive polypeptide, then there may be further additional amino acids on the N-terminus of the additional amino acid sequence stretch. Preferably these comprise the amino acid methionine (Met), or the sequence methionine, glycine and serine (Met-Gly-Ser).

[0047] This at least one additional amino acid sequence stretch preferably has the function to lead the inventive polypeptide through the outer membrane of bacteria and may have activity or may have no or only low activity when administered without being fused to the polypeptide of the invention. The function to guide the polypeptide through the outer membrane of Gram-negative bacteria is caused by the outer membrane or LPS disrupting, permeabilising or destabilizing activity of said amino acid sequence stretches.

[0048] Such outer membrane or LPS disrupting or permeabilising or destabilizing activity of these amino acid sequence stretches may be preferably determined in a method as follows: Exponentially growing Gram-negative cells are incubated at room temperature with protein (candidate polypeptide of the present invention exhibiting at least one additional amino acid sequence stretch) at a final concentration of 100 .mu.g/ml in buffer (20 mM NaH.sub.2PO.sub.4--NaOH pH7.4; 0.5 M NaCl; 0.5 M imidazole) and a cell density of .about.10.sup.6/ml. After 1 hour the cell suspension is diluted in PBS buffer (10.sup.-5, 10.sup.-4 and 10.sup.-3), plated (standard LB-medium) and incubated overnight at 37.degree. C. Additionally, negative controls containing cells in PBS buffer or cells incubated with the matching polypeptide without the additional amino acid sequence stretch) are plated. The residual colonies are counted after the overnight incubation for each plate. If the protein exhibits such outer membrane or LPS disrupting or permeabilising or destabilizing activity, the bacteria cells are lysed due to the treatment with the polypeptide and thus, the number of the bacteria colonies on the agar plate is reduced. Thus, the reduction in the number of bacteria colonies after treatment with the protein is indicative for an outer membrane or LPS disrupting or permeabilising or destabilizing activity of the polypeptide.

[0049] Especially preferred are cationic and/or polycationic amino acid sequence stretches comprising at least one motive according to SEQ ID NO:41 (KRKKRK). In particular cationic amino acid sequence stretches comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 or 17 motives according to SEQ ID NO: 41 (KRKKRK) are preferred. More preferred are cationic peptide stretches comprising at least one KRK motive (lys-arg-lys), preferable at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32 or 33 KRK motives.

[0050] In another preferred embodiment of the present invention the cationic amino acid sequence stretch comprises beside the positively charged amino acid residues, in particular lysine and/or arginine residues, neutrally charged amino acid residues, in particular glycine and/or serine residues. Preferred are cationic amino acid sequence stretches consisting of about 70% to about 100%, or about 80% to about 95%, or about 85% to about 90% positively charged amino acid residues, in particular lysine, arginine and/or histidine residues, more preferably lysine and/or arginine residues and of about 0% to about 30%, or about 5% to about 20%, or about 10% to about 20% neutrally charged amino acid residues, in particular glycine and/or serine residues. Preferred are amino acid sequence stretches consisting of about 4% to about 8% serine residues, of about 33% to about 36% arginine residues and of about 56% to about 63% lysine residues. Especially preferred are amino acid sequence stretches comprising at least one motive according to SEQ ID NO: 42 (KRXKR), wherein X is any other amino acid than lysine, arginine and histidine. Especially preferred are polypeptide stretches comprising at least one motive according to SEQ ID NO: 43 (KRSKR). More preferred are cationic stretches comprising at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or at least about 20 motives according to SEQ ID NO: 42 (KRXKR) or SEQ ID NO: 43 (KRSKR).

[0051] Also preferred are amino acid sequence stretches consisting of about 9 to about 16% glycine residues, of about 4 to about 11% serine residues, of about 26 to about 32% arginine residues and of about 47 to about 55% lysine residues. Especially preferred are amino acid sequence stretches comprising at least one motive according to SEQ ID NO: 44 (KRGSG). More preferred are cationic stretches comprising at least about 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or at least bout 20 motives according to SEQ ID NO: 44 (KRGSG).

[0052] In another preferred embodiment of the present invention such cationic amino acid sequence stretch comprises beside the positively charged amino acid residues, in particular lysine and/or arginine residues, hydrophobic amino acid residues, in particular valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues. Preferred are cationic amino acid sequence stretches consisting of about 70% to about 100%, or about 80% to about 95%, or about 85% to about 90% positively charged amino acid residues, in particular lysine and/or arginine residues and of about 0% to about 30%, or about 5% to about 20%, or about 10% to about 20% hydrophobic amino acid residues, valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues. Examples for cationic and polycationic amino acid sequence stretches are listed in the following table:

TABLE-US-00002 TABLE 2 SEQ ID amino acid sequence stretch length NO: KRKKRK 6 41 KRKKRKKRK 9 45 RRRRRRRRR 9 46 KKKKKKKK 8 47 KRKKRKKRKK 10 48 KRKKRKKRKKRK 12 49 KRKKRKKRKKRKKR 14 50 KKKKKKKKKKKKKKKK 16 51 KRKKRKKRKKRKKRKKRK 18 52 KRKKRKKRKKRKKRKKRKK 19 53 RRRRRRRRRRRRRRRRRRR 19 54 KKKKKKKKKKKKKKKKKKK 19 55 KRKKRKKRKRSKRKKRKKRK 20 56 KRKKRKKRKRSKRKKRKKRKK 21 57 KRKKRKKRKKRKKRKKRKKRK 21 58 KRKKRKKRKRGSGKRKKRKKRK 22 59 KRKKRKKRKRGSGSGKRKKRKKRK 24 60 KRKKRKKRKKRKKRKKRKKRKKRKK 25 61 KRKKRKKRKRSKRKKRKKRKRSKRK 31 62 KRKKRK KRKKRKKRKRGSGSGKRKKRKKRKG 38 63 SGSGKRKKRKKRK KRKKRKKRKKRKKRKKRKKRKKRKK 39 64 RKKRKKRKKRKKRK KRKKRKKRKRSKRKKRKKRKRSKRK 42 65 KRKKRKRSKRKKRKKRK

[0053] In a further aspect of the present invention at least one of the additional amino acid sequence stretches is an antimicrobial peptide, which comprises a positive net charge and around 50% hydrophobic amino acids. The antimicrobial peptides are amphipathic with a length of about 12 to about 50 amino acid residues. The antimicrobial peptides are naturally occurring in insects, fish, plants, arachnids, vertebrates or mammals. Preferably the antimicrobial peptide may be naturally occurring in radish, silk moth, wolf spider, frog, preferably in Xenopus laevis, Rana frogs, more preferably in Rana catesbeiana, toad, preferably Asian toad Bufo bufo gargarizans, fly, preferably in Drosophila, more preferably in Drosophila melanogaster, in Aedes aegypti, in honey bee, bumblebee, preferably in Bombus pascuorum, flesh fly, preferably in Sarcophaga peregrine, scorpion, horseshoe crab, catfish, preferably in Parasilurus asotus, cow, pig, sheep, porcine, bovine, monkey and human.

[0054] In another preferred embodiment of the present invention the antimicrobial amino acid sequence stretches consist of about 0% to about 5%, or about 0% to about 35%, or about 10% to about 35% or about 15% to about 45%, or about 20% to about 45% positively charged amino acid residues, in particular lysine and/or arginine residues and of about 50% to about 80%, or about 60% to about 80%, or about 55% to about 75%, or about 70% to about 90% hydrophobic amino acid residues, valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues.

[0055] In another preferred embodiment of the present invention the antimicrobial amino acid sequence stretches consist of about 4% to about 58% positively charged amino acid residues, in particular lysine and/or arginine residues and of about 33% to about 89% hydrophobic amino acid residues, valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues.

[0056] Examples for antimicrobial amino acid sequences which may be used in carrying out the present invention are listed in the following table.

TABLE-US-00003 TABLE 3 SEQ ID Peptide Sequence NO LL-37 LLGDFFRKSKEKIGKEFKRIVQRIKD 66 FLRNLVPRTES SMAP-29 RGLRRLGRKIAHGVKKYGPTVLRIIR 67 IAG Indolicidin ILPWKWPWWPWRR 68 Protegrin RGGRLCYCRRRFCVCVGR 69 Cecropin P1 SWLSKTAKKLENSAKKRISEGIAIAI 70 QGGPR Magainin GIGKFLHSAKKFGKAFVGEIMNS 71 Pleurocidin GWGSFFKKAAHVGKHVGKAALTHYL 72 Cecropin A GGLKKLGKKLEGAGKRVFNAAEKALP 73 (A.aegypti) VVAGAKALRK Cecropin A GWLKKIGKKIERVGQHTRDATIQGLG 74 (D. IPQQAANVAATARG melanogaster) Buforin II TRSSRAGLQFPVGRVHRLLRK 75 Sarcotoxin IA GWLKKIGKKIERVGQHTRDATIQGLG 76 IAQQAANVAATAR Apidaecin ANRPVYIPPPRPPHPRL 77 Ascaphine 5 GIKDWIKGAAKKLIKTVASHIANQ 78 Nigrocine 2 GLLSKVLGVGKKVLCGVSGLVC 79 Pseudin 1 GLNTLKKVFQGLHEAIKLINNHVQ 80 Ranalexin FLGGLIVPAMICAVTKKC 81 Melittin GIGAVLKVLTTGLPALISWIKRKRQQ 82 Lycotoxin 1 IWLTALKFLGKHAAKKLAKQQLSKL 83 Parasin 1 KGRGKQGGKVRAKAKTRSS 84 Buforin I AGRGKQGGKVRAKAKTRSSRAGLQFP 85 VGRVHRLLRKGNY Dermaseptin 1 ALWKTMLKKLGTMALHAGKAALGAAA 86 DTISQGTQ Bactenecin 1 RLCRIVVIRVCR 87 Thanatin GSKKPVPIIYCNRRTGKCQRM 88 Brevinin 1T VNPIILGVLPKVCLITKKC 89 Ranateurin 1 SMLSVLKNLGKVGLGFVACKINIKQC 90 Esculentin 1 GIFSKLGRKKIKNLLISGLKNVGKEV 91 GMDVVRTGIKIAGCKIKGEC Tachyplesin RWCFRVCYRGICYRKCR 92 Androctonin RSVCRQIKICRRRGGCYYKCTNRPY 93 alpha- DCYCRIPACIAGERRYGTCIYQGRLW 94 defensin AFCC beta- NPVSCVRNKGICVPIRCPGSMKQIGT 95 defensin CVGRAVKCCRKK theta- GFCRCLCRRGVCRCICTR 96 defensin defensin ATCDLLSGTGINHSACAAHCLLRGNR 97 (sapecin A) GGYCNGKAVCVCRN Thionin TTCCPSIVARSNFNVCRIPGTPEAIC 98 (crambin) ATYTGCIIIPGATCPGDYAN defensin from QKLCQRPSGTWSGVCGNNNACKNQCI 99 radish RLEKARHGSCNYVFPAHCICYFPC Drosomycin DCLSGRYKGPCAVWDNETCRRVCKEE 100 GRSSGHCSPSLKCWCEGC Hepcidin DTHFPICIFCCGCCHRSKCGMCCKT 101 Bac 5 RFRPPIRRPPIRPPFYPPFRPPIRPP 102 IFPPIRPPFRPPLGRPFP PR-39 RRRPRPPYLPRPRPPPFFPPRLPPRI 103 PPGFPPRFPPRFP Pyrrhocoricin VDKGSYLPRPTPPRPIYNRN 104 Histatin 5 DSHAKRHHGYKRKFHEKHHSHRGY 105 ECP19 RPPQFTRAQWFAIQHISLN 106 MSI-594 GIGKFLKKAKKGIGAVLKVLTTG 107 TL-ColM METLTVHAPSPSTNLPSYGNGAFSLS 108 APHVPGAGP SBO KLKKIAQKIKNFFAKLVA 109

[0057] In a further aspect of the present invention at least one of the additional amino acid sequence stretches may be a sushi peptide which is described by Ding J L, Li P, Ho B Cell Mol Life Sci. 2008 April; 65(7-8):1202-19. The Sushi peptides: structural characterization and mode of action against Gram-negative bacteria. Especially preferred is the sushi 1 peptide according to SEQ ID NO: 110.

[0058] Preferred sushi peptides are sushi peptides S1 and S3 and multiples thereof; FASEB J. 2000 September; 14(12):1801-13.

[0059] In a further aspect of the present invention at least one of the additional amino acid sequence stretches is a hydrophobic peptide, which comprises at least 90% of the hydrophobic amino acid residues of valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and/or glycine. In another preferred embodiment the hydrophobic peptide fused to the protein of the invention consists of about 90% to about 95%, or of about 90% to about 100%, or of about 95% to about 100% of the hydrophobic amino acid residues of valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and/or glycine.

[0060] Preferred hydrophobic peptides are Walmagh1 having the amino acid sequence according to SEQ ID NO: 111 and the hydrophobic peptide having the amino acid sequence Phe-Phe-Val-Ala-Pro (SEQ ID NO: 112).

[0061] In a further aspect of the present invention at least one of the additional amino acid sequence stretches is an amphipathic peptide, which comprises one or more of the positively charged amino acid residues of lysine, arginine and/or histidine, combined to one or more of the hydrophobic amino acid residues of valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and/or glycine. Side chains of the amino acid residues are oriented in order that cationic and hydrophobic surfaces are clustered at opposite sides of the peptide. Preferably, more than about 30, 40, 50, 60 or 70% of the amino acids in said peptide are positively charged amino acids. Preferably, more than about 30, 40, 50, 60 or 70%, of the amino acid residues in said peptide are hydrophobic amino acid residues. Advantageously, the amphipathic peptide is present at the N-terminal or the C-terminal end of the polypeptide according to the present invention.

[0062] In another embodiment of the invention, the amphipathic peptide consists of at least 5, more preferably at least of 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or at least 50 amino acid residues. In a preferred embodiment at least about 30, 40, 50, 60 or 70% of said amino acid residues of the amphipathic peptide are either arginine or lysine residues and/or at least about 30, 40, 50, 60 or 70% of said amino acid residues of the amphipathic peptide are of the hydrophobic amino acids valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and/or glycine.

[0063] In another preferred embodiment of the present invention the amphipathic peptide stretch comprises beside the positively charged amino acid residues, in particular lysine and/or arginine residues, hydrophobic amino acid residues, in particular valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues. Preferred are amphipathic peptide stretches consisting of about 10% to about 50%, or about 20% to about 50%, or about 30% to about 45% or about 5% to about 30% positively charged amino acid residues, in particular lysine and/or arginine residues and of about 50% to about 85%, or about 50% to about 90%, or about 55% to about 90%, or about 60% to about 90%, or about 65% to about 90% hydrophobic amino acid residues, valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues. In another preferred embodiment amphipathic peptide stretches consisting of 12% to about 50% positively charged amino acid residues, in particular lysine and/or arginine residues and of about 50% to about 85% hydrophobic amino acid residues, valine, isoleucine, leucine, methionine, phenylalanine, tryptophan, cysteine, alanine, tyrosine, proline and glycine residues, more preferably alanine, valine, leucine, isoleucine, phenylalanine, and/or tryptophan residues.

[0064] Preferred amphipathic peptides are .alpha.4-helix of T4 lysozyme according to SEQ ID NO: 113 and WLBU2-Variant having the amino acid sequence according to SEQ ID NO: 114 and Walmagh 2 according to SEQ ID NO: 115.

[0065] The optional additional amino acid sequence stretches as specified above consist preferably of at least 5, more preferably at least of 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or at least 100 amino acid residues. Especially preferred are those additional amino acid sequence stretches consisting of about 5 to about 100 amino acid residues, about 5 to about 50 or about 5 to about 30 amino acid residues. More preferred are peptide stretches consisting of about 6 to about 42 amino acid residues, about 6 to about 39 amino acid residues, about 6 to about 38 amino acid residues, about 6 to about 31 amino acid residues, about 6 to about 25 amino acid residues, about 6 to about 24 amino acid residues, about 6 to about 22 amino acid residues, about 6 to about 21 amino acid residues, about 6 to about 20 amino acid residues, about 6 to about 19 amino acid residues, about 6 to about 16 amino acid residues, about 6 to about 14 amino acid residues, about 6 to about 12 amino acid residues, about 6 to about 10 amino acid residues or about 6 to about 9 amino acid residues.

[0066] In a preferred embodiment the inventive polypeptide comprises at least one amino acid sequence stretch selected from the group consisting of KRK and SEQ ID NOs: 41-115.

[0067] An non-limiting example for a polypeptide according to the present invention comprising an additional amino acid sequence stretch is SEQ ID NO: 116, which comprises an N-terminal SMAP-29 peptide (SEQ ID NO: 67), a CBD deriving from KZ144 endolysin exhibiting 4 mutations (SEQ ID NO: 8) and a Lys68 endolysin sequence with a P78S mutation (SEQ ID NO: 26).

[0068] The additional amino acid sequence stretch of the polypeptide according to the present invention may be linked to the rest of the enzyme by intervening additional amino acid residues e.g. due to cloning reasons. Alternatively, the additional amino acid sequence stretches may be directly linked to the rest of the enzyme sequence without intervening linker sequences. The additional amino acid sequences, if more than one present in the inventive polypeptide and positioned on the same terminus of the enzyme, may likewise be linked to each other by additional intervening amino acid residues or may be directly joined to each other.

[0069] Preferably, said intervening additional amino acid residues may not be recognized and/or cleaved by proteases. Preferably said additional amino acid sequences are linked to each other and/or to the enzyme by at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 additional intervening amino acid residues.

[0070] In a preferred embodiment the at least one additional amino acid sequence stretch is linked to the rest of the inventive polypeptide, preferably at the N- or C-terminus of the polypeptide according to the present invention, by the additional intervening amino acid residues glycine, serine and serine (Gly-Ser-Ser), glycine, alanine, glycine and alanine (Gly-Ala-Gly-Ala; SEQ ID NO:117), glycine, alanine, glycine, alanine, glycine, alanine, glycine and alanine (Gly-Ala-Gly-Ala-Gly-Ala-Gly-Ala; SEQ ID NO:118) or glycine, alanine, glycine, alanine, glycine, alanine, glycine, alanine, glycine, alanine, glycine and alanine (Gly-Ala-Gly-Ala-Gly-Ala-Gly-Ala-Gly-Ala-Gly-Ala; SEQ ID NO:119).

[0071] Aside of the enzymatic domain (i.e. a domain having the activity of degrading the peptidoglycan of Gram-negative bacteria), the cell wall binding domain, and the optional additional amino acid sequence stretches, as defined herein, the inventive polypeptide may of course also comprise other amino acid sequence elements, e.g. one or more tags, e.g. a His-tag, Strep-tag, Avi-tag, Myc-tag, Gst-tag, JS-tag, cystein-tag, FLAG-tag or other tags known in the art, thioredoxin, maltose binding proteins (MBP) etc.

[0072] In this context, the inventive polypeptide, preferably having the ability of degrading the peptidoglycan layer of Gram negative bacteria, may additional comprise a tag e.g. for purification. Preferred is a His.sub.6-tag (SEQ ID NO: 120), preferably at the C-terminus and/or the N-terminus of the polypeptide according to the present invention. Said tag can be linked to the polypeptide by additional amino acid residues e.g. due to cloning reasons. Preferably said tag can be linked to the protein by at least 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 additional amino acid residues. Preferably said additional amino acid residues may not be recognized and/or cleaved by proteases. In a preferred embodiment the inventive polypeptide comprises a His.sub.6-tag at its C-terminus linked to the polypeptide by the additional amino acid residues lysine and glycine (Lys-Gly) or leucine and glutamic acid (Leu-Glu). Preferably, said additional amino acid residues may be not recognized or cleaved by proteases. In another preferred embodiment the inventive polypeptide comprises a His.sub.6-tag at its N-terminus linked to the polypeptide by the additional amino acid residues lysine and glycine (Lys-Gly) or leucine and glutamic acid (Leu-Glu). In another preferred embodiment the polypeptide comprises a His.sub.6-tag at its N- and C-terminus linked to the polypeptide by the additional amino acid residues lysine and glycine (Lys-Gly) or leucine and glutamic acid (Leu-Glu).

[0073] A polypeptide according to the present invention can be produced by standard means known in the art, e.g. by recombinant expression of nucleic acids encoding the respective polypeptide in appropriate host cells. If the inventive polypeptide comprises for example additionally amino acid sequence stretches or tags etc., such fusion proteins may be produced by linking the required individual nucleic acid sequences using standard cloning techniques as described e.g. by Sambrook et al. 2001, Molecular Cloning: A Laboratory Manual. Such a polypeptide may be produced likewise with methods known in the art, e.g., in recombinant DNA expression systems.

III. Nucleic Acids, Vectors, Bacteriophages and Host Cells

[0074] The present invention does also relate to nucleic acids encoding one or more inventive polypeptides of the present invention. The inventive nucleic acid may take all forms conceivable for a nucleic acid. In particular the nucleic acids according to the present invention may be RNA, DNA or hybrids thereof. They may be single-stranded or double-stranded. The may have the size of small transcripts or of entire genomes, such as a bacteriophage genome. As used herein, a nucleic acid encoding one or more inventive polypeptides of the present invention may be a nucleic acid reflecting the sense strand. Likewise, the antisense strand is also encompassed. The nucleic acid may encompass a heterologous promotor for expression of the inventive polypeptide.

[0075] In a further aspect the present invention relates to a vector comprising a nucleic acid according to the present invention. Such vector may for example be an expression vector allowing for expression of an inventive polypeptide. Said expression may be constitutive or inducible. The vector may also be a cloning vector comprising the nucleic acid sequence of the current invention for cloning purposes.

[0076] The present invention does also relate to a bacteriophage comprising an inventive nucleic acid, in particular comprising an inventive nucleic acid encoding a polypeptide according to the present invention.

[0077] The present invention does also relate to (isolated) host cells comprising a polypeptide, nucleic acid, vector, or bacteriophage according to the present invention. The host cells may be selected in particular from the group consisting of bacterial cells and yeast cells. Where appropriate, other suitable host cells may be immortalized cell lines, e.g. of mammalian (in particular human) origin. Particularly preferred host cells comprise a polypeptide according to the present invention.

IV. Compositions

[0078] In a further aspect the present invention relates to a composition comprising a polypeptide according to the present invention, a nucleic acid according to the present invention, a vector according to the present invention, a bacteriophage according to the present invention and/or a host cell according to the present invention.

[0079] A composition according to the present invention may be a pharmaceutical composition comprising a pharmaceutical acceptable diluent, excipient or carrier.

[0080] In an even further aspect the composition according to the present invention is a cosmetic composition. Several bacterial species can cause irritations on environmentally exposed surfaces of the patient's body such as the skin. In order to prevent such irritations or in order to eliminate minor manifestations of said bacterial pathogens, special cosmetic preparations may be employed, which comprise sufficient amounts of the inventive polypeptide, nucleic acid, vector, host cell and/or composition in order to achieve a comedolytic effect.

V. Uses

[0081] In a further aspect the present invention relates to a polypeptide according to the present invention, a nucleic acid according to the present invention, a vector according to the present invention, a bacteriophage according to the present invention, a host cell according to the present invention, and/or a composition according to the present invention for use in a method of treatment of the human or animal body by surgery or therapy or in diagnostic methods practiced on the human or animal body. In such scenarios the antibacterial activity of polypeptide of the present invention can be exploited, in particular if the polypeptide comprises the additional amino acid sequence stretch specified above.

[0082] Such method typically comprises administering to a subject an effective amount of an inventive polypeptide, nucleic acid, vector, bacteriophage, host cell or a composition. The subject may for example be a human or an animal, with human subjects being more preferred. In particular, the inventive polypeptide, the inventive nucleic acid, the inventive vector, the inventive bacteriophage, the inventive host cell, and/or the inventive composition may be used in methods for the treatment or prevention of bacterial infections, such Gram-negative bacterial infections.

[0083] The dosage and route of administration used in a method of treatment (or prophylaxis) according to the present invention depends on the specific disease/site of infection to be treated. The route of administration may be for example oral, topical, nasopharyngeal, parenteral, intravenous, rectal or any other route of administration.

[0084] For application of an inventive polypeptide, nucleic acid, vector, bacteriophage, host cell or composition to a site of infection (or site endangered to be infected) a formulation may be used that protects the active compounds from environmental influences such as proteases, oxidation, immune response etc., until it reaches the site of infection. Therefore, the formulation may be capsule, dragee, pill, suppository, injectable solution or any other medical reasonable galenic formulation. Preferably, the galenic formulation may comprise suitable carriers, stabilizers, flavourings, buffers or other suitable reagents. For example, for topical application the formulation may be a lotion or plaster, for nasopharyngeal application the formulation may be saline solution to be applied via a spray to the nose.

[0085] Preferably, an inventive polypeptide, nucleic acid, vector, bacteriophage, host cell or composition is used in combination with other conventional antibacterial agents, such as antibiotics, lantibiotics, bacteriocins or endolysins, etc. The administration of the conventional antibacterial agent can occur prior to, concurrent with or subsequent to administration of the inventive polypeptide, nucleic acid, vector, bacteriophage, host cell or composition.

[0086] In a further aspect the present invention relates to the inventive polypeptide, nucleic acid, vector, bacteriophage, host cell or composition for use as diagnostic means in medical diagnostics, food diagnostics, feed diagnostics, or environmental diagnostics, in particular as a diagnostic means for the diagnostic of bacterial infection, in particular those caused by Gram-negative bacteria. In this respect the inventive polypeptide, nucleic acid, vector, host cell or composition may be used as a tool to specifically degrade the peptidoglycan of pathogenic bacteria, in particular of Gram-negative pathogenic bacteria. The degradation of the bacterial cells by the inventive polypeptide, nucleic acid, vector, host cell or composition can be supported by the addition of detergents like Triton X-100 or other additives which weaken the bacterial cell envelope like polymyxin B. Specific cell degradation is needed as an initial step for subsequent specific detection of bacteria using nucleic acid based methods like PCR, nucleic acid hybridization or NASBA (Nucleic Acid Sequence Based Amplification), immunological methods like IMS, immunofluorescence or ELISA techniques, or other methods relying on the cellular content of the bacterial cells like enzymatic assays using proteins specific for distinct bacterial groups or species (e.g. .beta.-galactosidase for enterobacteria, coagulase for coagulase positive strains).

[0087] In a further aspect the present invention relates to the use of the inventive polypeptide, the inventive nucleic acid, the inventive vector, the inventive bacteriophage, the inventive host cell, and/or the inventive composition, as an antimicrobial in food, feed, or cosmetics, or use as disinfecting agent. They can be used in particular for the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of (inanimate) surfaces coming into contact with foodstuff (such as shelves and food deposit areas), of feedstuff, of feed processing equipment, of feed processing plants, of (inanimate) surfaces coming into contact with feedstuff (such as shelves and feed deposit areas), of medical devices, or of (inanimate) surfaces in hospitals, doctor's offices and other medical facilities.

BRIEF DESCRIPTION OF THE FIGURES

[0088] In the following a brief description of the appended figures will be given. The figures are intended to illustrate the present invention in more detail. However, they are not intended to limit the scope of the invention to these specific examples.

[0089] FIG. 1: illustrates an archetypical structure of a Gram-negative modular endolysin. Depicted is the 3D structure of KZ 144 endolysin (Fokine et al. J. Biol. Chem. 2008, 283:7242-7250). The cell wall binding domain (CBD) is a domain (see top of FIG.) clearly separate from the enzymatically active domain (bottom of structure).

[0090] FIG. 2: illustrates the structure of a Gram-negative globular endolysin. Depicted is the modelled 3D structure of Lys394 endolysin. The structure has been generated by way of homology modeling on basis of the structure of T5 lysozyme, which shares a 97% sequence identity with Lys394. A domain architecture, as can be seen for, e.g. KZ 144 endolysin, can not be found in the structure of Lys394 endolysin.

[0091] FIG. 3: illustrates muralytic activity for Salmonella phage endolysin Lys68 and two chimeric variants thereof (cEL188-Lys68; cKZ144-Lys68) on chloroform treated P. aeruginosa cells. The fusion proteins exhibiting the additional heterologous CBD show increased activity.

[0092] FIG. 4: illustrates muralytic activity for ABgp46 endolysin and two chimeric variants thereof (cKZ144-ABgp46; cICP-ABgp46) on chloroform treated P. aeruginosa cells. The fusion proteins exhibiting the additional heterologous CBD show increased activity.

[0093] FIG. 5: illustrates muralytic activity for Lys394 endolysin and a chimeric variant thereof (cICP-5394) on chloroform treated P. aeruginosa cells. The fusion protein exhibiting the additional heterologous CBD shows increased activity.

VI. Examples

[0094] In the following, specific examples illustrating various embodiments and aspects of the invention are presented. However, the present invention shall not to be limited in scope by the specific embodiments described herein. Indeed, various modifications of the invention in addition to those described herein will become readily apparent to those skilled in the art from the foregoing description, accompanying figures and the examples below. All such modifications fall within the scope of the appended claims.

Example 1: Variants of Lys68 Endolysin Having an Additional Gram-Negative CBD Exhibit Increased Muralytic Activity

[0095] Two variants of Salmonella phage endolysin Lys68 were generated. The first variant is a fusion with a sequence comprising the CBD of EL188 endolysin (SEQ ID NO:12). The resulting chimeric variant cEL188-Lys68 comprises SEQ ID NO:29. The second variant is a fusion with the CBD of KZ144 endolysin (SEQ ID NO:7) The CBD used exhibits three point mutations in the sequence of the KZ144 CBD in which cysteine residues were replaced by serine residues. The resulting chimeric variant cKZ144-Lys68 comprises SEQ ID NO:30. The wildtype endolysin and its chimeric variants were expressed in E. coli. Subsequently, the proteins were purified. In order to test the muralytic activity of the enzymes, Pseudomonas aeruginosa PAO1 cells were treated with chloroform to remove the outer membrane. Therefore, 20 mM HEPES pH 7.4, 150 mM NaCl buffer was saturated with chloroform. Exponentially growing P. aeruginosa cells were harvested and resuspended in chloroform buffer and incubated for 45 minutes. Afterwards, the cells were washed two times in 20 mM HEPES pH 7.4 and 150 mM NaCl and subsequently diluted with the same buffer to a final OD600 of about 0.8. Subsequently, each protein was added at a final concentration of 0.005 .mu.M to an aliquot of 1 ml cell solution and the reduction of the OD600 was recorded over a period of 1800 seconds.

[0096] The two chimeric variants of Lys68 endolysin having an additional Gram-negative CBD exhibit significantly increased muralytic activity in comparison to the wildtype endolysin (see FIG. 3).

Example 2: Variants of ABgp46 Endolysin Having an Additional Gram-Negative CBD Exhibit Increased Muralytic Activity

[0097] Two variants of ABgp46 endolysin (deriving from Acinetobacter phage vB_AbaP_CEB1) were generated. The first variant is a fusion with the CBD of KZ144 endolysin (SEQ ID NO: 7). The resulting chimeric variant cKZ144-ABgp46 comprises SEQ ID NO:31. The second variant is a fusion with the CBD of the baseplate tail protein of Vibrio phage ICP1 (SEQ ID NO:14). The resulting chimeric variant cICP-ABgp46 comprises SEQ ID NO:32. The wildtype endolysin and its chimeric variants were expressed in E. coli. Subsequently, the proteins were purified. In order to test the muralytic activity of the enzymes, Pseudomonas aeruginosa PAO1 cells were treated with chloroform to remove the outer membrane. Therefore, 20 mM HEPES pH 7.4, 150 mM NaCl buffer was saturated with chloroform. Exponentially growing P. aeruginosa cells were harvested and resuspended in chloroform buffer and incubated for 45 minutes. Afterwards, the cells were washed two times in 20 mM HEPES pH 7.4 and 150 mM NaCl and subsequently diluted with the same buffer to a final OD600 of about 0.8. Subsequently, each protein was added at a final concentration of 0.005 .mu.M to an aliquot of 1 ml cell solution and the reduction of the OD600 was recorded over a period of 1800 seconds.

[0098] The two chimeric variants of ABgp46 endolysin having an additional Gram-negative CBD exhibit significantly increased muralytic activity in comparison to the wildtype endolysin (see FIG. 5).

Example 3: Variant of Lys394 Endolysin Having an Additional CBD Exhibits De Novo Muralytic Activity on P. aeruginosa

[0099] A variant of Salmonella phage endolysin Lys394 was generated. The variant is a fusion with the CBD of ICP tail/baseplate protein (SEQ ID NO: 14). The resulting chimeric variant comprises SEQ ID NO:33. The wildtype endolysin and its chimeric variant were expressed in E. coli. Subsequently, the proteins were purified. In order to test the muralytic activity of the enzymes, Pseudomonas aeruginosa PAO1 cells were treated with chloroform to remove the outer membrane. Therefore, 20 mM HEPES pH 7.4, 150 mM NaCl buffer was saturated with chloroform. Exponentially growing P. aeruginosa cells were harvested and resuspended in chloroform buffer and incubated for 45 minutes. Afterwards, the cells were washed two times in 20 mM HEPES pH 7.4 and 150 mM NaCl and subsequently diluted with the same buffer to a final OD600 of about 0.8. Subsequently, each protein was added at a final concentration of 0.005 .mu.M to an aliquot of 1 ml cell solution and the reduction of the OD600 was recorded over a period of 1800 seconds.

[0100] In contrast to the wildtype endolysin, the chimeric variant of Lys394 endolysin exhibits significant muralytic activity on P. aeruginosa cells (see FIG. 6) already at a concentration of 0.005 .mu.M.

Example 4: Fusion Protein Exhibiting Lys68 Endolysin Variant, a CBD Variant of the KZ144 Endolysin CBD and an Additional Antimicrobial Peptide SMAP-29 Exhibits Antibacterial Activity Against a Broad Range of Gram-Negative Bacteria

[0101] In a further experiment compatibility of a chimeric endolysin according to the present invention with an antimicrobial peptide to effectively kill Gram-negative bacteria was tested. For this purpose, a fusion protein was generated which comprises the components SMAP-29 (SEQ ID NO: 67), a derivative of the KZ144 CBD exhibiting four mutations (SEQ ID NO: 8) and a derivative of Lys68 endolysin (SEQ ID NO: 26). The resulting polypeptide comprises the consecutive sequence of SEQ ID NO: 116. Due to the mutations in SEQ ID NO: 8 and 26, the resulting fusion protein exhibited improved thermal stability. The chimeric variant was expressed in E. coli.

[0102] Briefly, bacteria were grown in (Luria-Bertani) medium and diluted 1:10 in Mueller-Hinton medium. At optical density OD.sub.600 of about 0.6 bacteria were diluted in the same medium 1:10 followed by a 1:500 dilution. Protein buffer (20 mM HEPES, 500 mM NaCl, pH 7.4) and proteins were pipetted into a 96 well plate, using different concentrations of proteins and an end volume of 20 .mu.l including 500 .mu.M EDTA final concentration (if required). 180 .mu.l of bacterial cells or a medium (Mueller-Hinton) control were given to the 96 well plate and mixed. The plate was incubated for 18-22 hours at 37.degree. C. and the bacterial growth was determined measuring the OD600 values of the wells. The MIC which is the protein concentration of the well which showed the same OD600 value as the no-bacteria control was determined.

[0103] The fusion protein showed antibacterial activity against a surprisingly broad range of Gram-negative bacteria including Pseudomonas, Klebsiella, Escherichia, Acinetobacter and Salmonella species. The corresponding results are described in table 4.

TABLE-US-00004 TABLE 4 NW values MIC Bacterial strain EDTA (.mu.g/ml) S2 Pseudomonas aeruginosa Br667 0.5 mM 10 S84 Pseudomonas aeruginosa Aa249 0.5 mM 10 S53 Klebsiella pneumoniae B10-03.05.700 0.5 mM 20 S516 Klebsiella pneumoniae va32842 0.5 mM 17.5 S441 Escherichia coli B12-11.20.0192 0.5 mM 5 S458 Escherichia coli B12-11.30.0188 0.5 mM 5 S45 Acinetobacter baumannii 2671 0.0 mM 10 S138 Acinetobacter baumannii NRZ-00066 0.0 mM 10 S795 Salmonella Enteritidis LGL-238 0.5 mM 7.5 S25 Salmonella Thyphimurium DSM 17058 0.5 mM 15

[0104] In addition, the fusion protein comprising SEQ ID NO: 116 exhibited significant thermal stability due to the mutations K59M and P78S in the sequence of Lys68 endolysin.

Sequence CWU 1

1

120161PRTArtificialConsensus SequenceMISC_FEATURE(1)..(1)Xaa can be any naturally occurring amino acid or absent; in particular it can be methionineMISC_FEATURE(6)..(6)Xaa can be any naturally occurring amino acid, in particular cysteine, serine, arginine or aspargine, preferably serine, arginine or aspargine, most preferaby serineMISC_FEATURE(15)..(15)Xaa can be any naturally occurring amino acid, in particular cysteine, serine, arginine or aspargine, preferably serine, arginine or aspargine, most preferaby serineMISC_FEATURE(42)..(42)Xaa can be any naturally occurring amino acid, in particular cysteine, serine, arginine or aspargine, preferably serine, arginine or aspargine, most preferaby serineMISC_FEATURE(51)..(51)Xaa can be any naturally occurring amino acid, in particular lysine or methionine 1Arg Gly Asp Glu Val Xaa Gln Leu Gln Thr Leu Leu Asn Leu Xaa Gly1 5 10 15Tyr Asp Val Gly Lys Pro Asp Gly Ile Phe Gly Asn Asn Thr Phe Asn 20 25 30Gln Val Val Lys Phe Gln Lys Asp Asn Xaa Leu Asp Ser Asp Gly Ile 35 40 45Val Gly Xaa Asn Thr Trp Ala Glu Leu Phe Ser Lys Tyr 50 55 60270PRTArtificialConsensus SequenceMISC_FEATURE(1)..(1)Xaa can be any naturally occurring amino acid or absent; in particular it can be methionineMISC_FEATURE(14)..(14)Xaa can be any naturally occurring amino acid, in particular cysteine, serine, arginine or aspargine, preferably serine, arginine or aspargine, most preferaby serineMISC_FEATURE(23)..(23)Xaa can be any naturally occurring amino acid, in particular cysteine, serine, arginine or aspargine, preferably serine, arginine or aspargine, most preferaby serineMISC_FEATURE(50)..(50)Xaa can be any naturally occurring amino acid, in particular cysteine, serine, arginine or aspargine, preferably serine, arginine or aspargine, most preferaby serineMISC_FEATURE(59)..(59)Xaa can be any naturally occurring amino acid, in particular lysine or methionine 2Xaa Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Xaa Gln Leu1 5 10 15Gln Thr Leu Leu Asn Leu Xaa Gly Tyr Asp Val Gly Lys Pro Asp Gly 20 25 30Ile Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp 35 40 45Asn Xaa Leu Asp Ser Asp Gly Ile Val Gly Xaa Asn Thr Trp Ala Glu 50 55 60Leu Phe Ser Lys Tyr Ser65 70361PRTunknownCBD of phiKZgp144 3Arg Gly Asp Glu Val Cys Gln Leu Gln Thr Leu Leu Asn Leu Cys Gly1 5 10 15Tyr Asp Val Gly Lys Pro Asp Gly Ile Phe Gly Asn Asn Thr Phe Asn 20 25 30Gln Val Val Lys Phe Gln Lys Asp Asn Cys Leu Asp Ser Asp Gly Ile 35 40 45Val Gly Lys Asn Thr Trp Ala Glu Leu Phe Ser Lys Tyr 50 55 60461PRTArtificial sequenceAmino acids 9 to 69 of endolysin KZ144 (CBD) with three cysteines replaced by serine 4Arg Gly Asp Glu Val Ser Gln Leu Gln Thr Leu Leu Asn Leu Ser Gly1 5 10 15Tyr Asp Val Gly Lys Pro Asp Gly Ile Phe Gly Asn Asn Thr Phe Asn 20 25 30Gln Val Val Lys Phe Gln Lys Asp Asn Ser Leu Asp Ser Asp Gly Ile 35 40 45Val Gly Lys Asn Thr Trp Ala Glu Leu Phe Ser Lys Tyr 50 55 60561PRTArtificial sequenceAmino acids 9 to 69 of endolysin KZ144 (CBD) with three cysteines replaced by serine and one lysine replaced by methionine 5Arg Gly Asp Glu Val Ser Gln Leu Gln Thr Leu Leu Asn Leu Ser Gly1 5 10 15Tyr Asp Val Gly Lys Pro Asp Gly Ile Phe Gly Asn Asn Thr Phe Asn 20 25 30Gln Val Val Lys Phe Gln Lys Asp Asn Ser Leu Asp Ser Asp Gly Ile 35 40 45Val Gly Met Asn Thr Trp Ala Glu Leu Phe Ser Lys Tyr 50 55 60669PRTunknownN-terminus incl. CBD of phiKZgp144 (aa 2-70) 6Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Cys Gln Leu Gln1 5 10 15Thr Leu Leu Asn Leu Cys Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 20 25 30Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn 35 40 45Cys Leu Asp Ser Asp Gly Ile Val Gly Lys Asn Thr Trp Ala Glu Leu 50 55 60Phe Ser Lys Tyr Ser65769PRTArtificial sequenceAmino acids 2 to 70 of endolysin KZ144 with three cysteines replaced by serine 7Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu Gln1 5 10 15Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 20 25 30Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn 35 40 45Ser Leu Asp Ser Asp Gly Ile Val Gly Lys Asn Thr Trp Ala Glu Leu 50 55 60Phe Ser Lys Tyr Ser65869PRTArtificial sequenceAmino acids 2 to 70 of endolysin KZ144 with three cysteines replaced by serine and one lysine replaced by methionine 8Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu Gln1 5 10 15Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 20 25 30Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn 35 40 45Ser Leu Asp Ser Asp Gly Ile Val Gly Met Asn Thr Trp Ala Glu Leu 50 55 60Phe Ser Lys Tyr Ser65991PRTArtificial sequenceConsensus SequenceMISC_FEATURE(40)..(40)Xaa can be any naturally occurring amino acid; in particular it can be glycine or tryptophan 9Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly1 5 10 15Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser 20 25 30Ser Thr Glu Thr Leu Leu Arg Xaa Tyr Ala Glu Val Val Gly Lys Asn 35 40 45Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn 50 55 60Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser65 70 75 80Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr 85 901091PRTUnknownELgp188 10Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly1 5 10 15Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser 20 25 30Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val Gly Lys Asn 35 40 45Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn 50 55 60Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser65 70 75 80Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr 85 901191PRTUnknownELgp188 11Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys Glu Leu Gly1 5 10 15Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly Thr Ser Ser 20 25 30Ser Thr Glu Thr Leu Leu Arg Trp Tyr Ala Glu Val Val Gly Lys Asn 35 40 45Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser Gly Tyr Asn 50 55 60Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly Leu Tyr Ser65 70 75 80Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr 85 9012113PRTUnknownELgp188 12Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys1 5 10 15Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 20 25 30Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 35 40 45Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 50 55 60Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly65 70 75 80Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 85 90 95Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 100 105 110Thr13113PRTUnknownWP_050469949.1 2-114 13Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys1 5 10 15Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 20 25 30Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Trp Tyr Ala Glu Val Val 35 40 45Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 50 55 60Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly65 70 75 80Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 85 90 95Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 100 105 110Thr1478PRTunknownCBD of tail baseplate protein of Vibrio phage ICP1 14Ile Leu Lys Arg Gly Ser Ser Gly Ala Asp Val Lys Asn Met Gln Glu1 5 10 15Tyr Leu Thr Ala Leu Gly Tyr Asp Thr Lys Gly Val Glu Gly Thr Phe 20 25 30Glu Gly Gly Thr Glu Ser Ala Val Lys Ala Phe Gln Lys Asp Met Ser 35 40 45Phe Thr Val Val Asp Gly Ile Ile Gly Asn Gln Thr Ala Lys His Leu 50 55 60Val Asp Met Tyr Tyr Gly Lys Val Val Pro Phe Gly Tyr Val65 70 7515111PRTunknownCBD of OBPgpLYS endolysin 15Asn Ala Ser Ile Ile Met Ser Ile Gln Arg Thr Leu Ala Ser Leu Ser1 5 10 15Leu Tyr Gly Gly Arg Ile Asp Gly Leu Phe Gly Glu Lys Cys Arg Gly 20 25 30Ala Ile Ile Leu Met Leu Asn Lys Val Tyr Pro Asn Phe Ser Thr Asn 35 40 45Lys Leu Pro Ser Asn Thr Tyr Glu Ala Glu Ser Val Phe Thr Phe Leu 50 55 60Gln Thr Ala Leu Ala Gly Val Gly Leu Tyr Thr Ile Thr Ile Asp Gly65 70 75 80Lys Trp Gly Gly Thr Ser Gln Gly Ala Ile Asp Ala Leu Val Lys Ser 85 90 95Tyr Arg Gln Ile Thr Glu Ala Glu Arg Ala Gly Ser Thr Leu Pro 100 105 1101636PRTunknownCBD of PVP-SE1gp146 endolysin 16Ala Ala Ile Ala Glu Ile Gln Arg Met Leu Ile Glu Gly Gly Phe Ser1 5 10 15Val Gly Lys Ser Gly Ala Asp Gly Leu Tyr Gly Pro Ala Thr Lys Ala 20 25 30Ala Leu Gln Lys 351756PRTunknownCBD of endolysin 201phi2-1gp229 of Pseudomonas chlororaphis phage 201phi2-1 17Lys Gly Asp Asp Val Ile Arg Leu Gln Arg Lys Leu Ile Gly Leu Gly1 5 10 15Tyr Ser Val Lys Asp Asp Gly Val Phe Gly Asp Asn Thr Glu Lys Ala 20 25 30Val Lys Ala Val Gln Leu Arg Phe Asn Leu Lys Asp Asp Gly Ile Val 35 40 45Gly Asn Asn Thr Trp Ala Val Leu 50 5518186PRTArtificial Sequencepeptidoglycan degrading enzyme 18Leu Ser Glu Lys Ser Phe Val Glu Ala Ala Ala Ser Leu Gly Cys Glu1 5 10 15Val Ala Ala Ile Lys Ala Ile Ala Ser Val Glu Thr Lys Gly Ser Ala 20 25 30Trp Ile Thr Pro Gly Val Pro Gln Ile Leu Tyr Glu Arg His Ile Met 35 40 45Ala Arg Leu Leu Lys Ala Lys Gly Val Pro Ile Ala Gly Leu Pro Ser 50 55 60Asp Leu Val Asn Thr Thr Pro Gly Gly Tyr Gly Lys Phe Ser Glu Gln65 70 75 80His Gly Lys Leu Asp Arg Ala Val Lys Ile Asp Arg Glu Cys Ala Leu 85 90 95Gln Ser Cys Ser Trp Gly Met Phe Gln Leu Met Gly Phe Asn Tyr Lys 100 105 110Leu Cys Gly Tyr Ala Thr Val Gln Ala Phe Val Asn Ala Met Tyr Lys 115 120 125Ser Glu Asp Glu Gln Leu Asn Ala Phe Val Gly Phe Ile Lys Ser Asn 130 135 140Leu Gln Leu Asn Asp Ala Leu Lys Ser Lys Asp Trp Ala Thr Val Ala145 150 155 160Arg Leu Tyr Asn Gly Ala Asp Tyr Lys Ile Asn Ser Tyr Asp Gln Lys 165 170 175Leu Ala Val Ala Tyr Glu Ser Asn Lys Arg 180 18519184PRTunknownendolysin of Acinetobacter phage vB_AbaP_CEB1, w/o N.terminal methionine 19Ile Leu Thr Lys Asp Gly Phe Gly Ile Ile Arg Asn Glu Leu Phe Gly1 5 10 15Gly Lys Leu Asp Gln Thr Gln Val Asp Ala Ile Asn Phe Ile Val Glu 20 25 30Lys Ala Thr Glu Ser Gly Leu Ser Tyr Pro Glu Ala Ala Tyr Leu Leu 35 40 45Ala Thr Ile Tyr His Glu Thr Gly Leu Pro Ser Gly Tyr Arg Thr Met 50 55 60Gln Pro Ile Lys Glu Ala Gly Ser Asp Asn Tyr Leu Arg Ser Lys Lys65 70 75 80Tyr Tyr Pro Tyr Ile Gly Tyr Gly Tyr Val Gln Leu Thr Trp Lys Glu 85 90 95Asn Tyr Gly Arg Ile Gly Lys Leu Ile Gly Ile Asp Leu Ile Lys Asn 100 105 110Pro Glu Lys Ala Leu Glu Pro Leu Ile Ala Ile Gln Ile Ala Ile Lys 115 120 125Gly Met Leu Asn Gly Trp Phe Thr Gly Val Gly Phe Arg Arg Lys Arg 130 135 140Pro Val Ser Lys Tyr Asn Lys Gln Gln Tyr Ile Ala Ala Arg Asn Ile145 150 155 160Ile Asn Gly Lys Asp Lys Ala Glu Leu Ile Ala Lys Tyr Ala Ile Ile 165 170 175Phe Glu Arg Ala Leu Arg Ser Leu 18020185PRTunknownendolysin of Acinetobacter phage vB_AbaP_CEB1 20Met Ile Leu Thr Lys Asp Gly Phe Gly Ile Ile Arg Asn Glu Leu Phe1 5 10 15Gly Gly Lys Leu Asp Gln Thr Gln Val Asp Ala Ile Asn Phe Ile Val 20 25 30Glu Lys Ala Thr Glu Ser Gly Leu Ser Tyr Pro Glu Ala Ala Tyr Leu 35 40 45Leu Ala Thr Ile Tyr His Glu Thr Gly Leu Pro Ser Gly Tyr Arg Thr 50 55 60Met Gln Pro Ile Lys Glu Ala Gly Ser Asp Asn Tyr Leu Arg Ser Lys65 70 75 80Lys Tyr Tyr Pro Tyr Ile Gly Tyr Gly Tyr Val Gln Leu Thr Trp Lys 85 90 95Glu Asn Tyr Gly Arg Ile Gly Lys Leu Ile Gly Ile Asp Leu Ile Lys 100 105 110Asn Pro Glu Lys Ala Leu Glu Pro Leu Ile Ala Ile Gln Ile Ala Ile 115 120 125Lys Gly Met Leu Asn Gly Trp Phe Thr Gly Val Gly Phe Arg Arg Lys 130 135 140Arg Pro Val Ser Lys Tyr Asn Lys Gln Gln Tyr Ile Ala Ala Arg Asn145 150 155 160Ile Ile Asn Gly Lys Asp Lys Ala Glu Leu Ile Ala Lys Tyr Ala Ile 165 170 175Ile Phe Glu Arg Ala Leu Arg Ser Leu 180 18521162PRTSalmonella phage phi68 21Met Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala1 5 10 15Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys 20 25 30Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu Gly 35 40 45Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp Met 50 55 60Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Pro Leu Asn65 70 75 80Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn Ala Gly Val 85 90 95Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly 100 105 110Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln Asn 115 120 125Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly Arg Val Ala 130 135 140Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile Gly Arg Gly145 150 155 160Ala Lys22132PRTSalmonella phage phi68 22Met Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala1 5 10 15Phe Glu Gly

Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys 20 25 30Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu Gly 35 40 45Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp Met 50 55 60Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Pro Leu Asn65 70 75 80Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn Ala Gly Val 85 90 95Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly 100 105 110Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln Asn 115 120 125Gly Lys Ser Leu 13023148PRTSalmonella phage phi68 23Met Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala1 5 10 15Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys 20 25 30Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu Gly 35 40 45Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp Met 50 55 60Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Pro Leu Asn65 70 75 80Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn Ala Gly Val 85 90 95Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly 100 105 110Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln Asn 115 120 125Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly Arg Val Ala 130 135 140Leu Phe Asp Gly14524156PRTSalmonella phage phi68 24Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala Phe Glu Gly Phe Arg Gly1 5 10 15Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys Tyr Leu Thr Ile Gly Tyr 20 25 30Gly His Tyr Gly Ala Asp Val Lys Glu Gly Gln Lys Ile Thr Glu Gly 35 40 45Gln Gly Leu Leu Leu Leu His Lys Asp Met Val Lys Ala Val Ala Ala 50 55 60Val Asp Ala Val Ala His Pro Pro Leu Asn Gln Ser Gln Phe Asp Ala65 70 75 80Met Cys Asp Leu Val Tyr Asn Ala Gly Val Gly Val Ile Ala Ala Ser 85 90 95Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly Asp Val Ala Thr Leu Arg 100 105 110Asn Lys Leu Thr Gln Phe His Tyr Gln Asn Gly Lys Ser Leu Leu Gly 115 120 125Leu Arg Arg Arg Ala Ala Gly Arg Val Ala Leu Phe Asp Gly Met Leu 130 135 140Trp Gln Gln Ala Glu Ala Ile Gly Arg Gly Ala Lys145 150 15525161PRTSalmonella phage phi68 25Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala Phe1 5 10 15Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys Tyr 20 25 30Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu Gly Gln 35 40 45Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp Met Val 50 55 60Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Pro Leu Asn Gln65 70 75 80Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn Ala Gly Val Gly 85 90 95Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly Asp 100 105 110Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln Asn Gly 115 120 125Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly Arg Val Ala Leu 130 135 140Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile Gly Arg Gly Ala145 150 155 160Lys26161PRTArtificial sequenceLys68 (P78S) w/o Met 26Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala Phe1 5 10 15Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys Tyr 20 25 30Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu Gly Gln 35 40 45Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp Met Val 50 55 60Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Ser Leu Asn Gln65 70 75 80Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn Ala Gly Val Gly 85 90 95Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly Asp 100 105 110Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln Asn Gly 115 120 125Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly Arg Val Ala Leu 130 135 140Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile Gly Arg Gly Ala145 150 155 160Lys27136PRTUnknownS394 endolysin without N-terminal methionine 27Ser Phe Lys Phe Gly Lys Asn Ser Glu Lys Gln Leu Ala Thr Val Lys1 5 10 15Pro Glu Leu Gln Lys Val Ala Arg Arg Ala Leu Glu Leu Ser Pro Tyr 20 25 30Asp Phe Thr Ile Val Gln Gly Ile Arg Thr Val Ala Gln Ser Ala Gln 35 40 45Asn Ile Ala Asn Gly Thr Ser Phe Leu Lys Asp Pro Ser Lys Ser Lys 50 55 60His Val Thr Gly Asp Ala Ile Asp Phe Ala Pro Tyr Ile Asn Gly Lys65 70 75 80Ile Asp Trp Lys Asp Leu Glu Ala Phe Trp Ala Val Lys Lys Ala Phe 85 90 95Glu Gln Ala Gly Lys Glu Leu Gly Ile Lys Leu Arg Phe Gly Ala Asp 100 105 110Trp Asn Ser Ser Gly Asp Tyr His Asp Glu Ile Asp Arg Gly Thr Tyr 115 120 125Asp Gly Gly His Val Glu Leu Val 130 13528137PRTBacteriophage S-394 28Met Ser Phe Lys Phe Gly Lys Asn Ser Glu Lys Gln Leu Ala Thr Val1 5 10 15Lys Pro Glu Leu Gln Lys Val Ala Arg Arg Ala Leu Glu Leu Ser Pro 20 25 30Tyr Asp Phe Thr Ile Val Gln Gly Ile Arg Thr Val Ala Gln Ser Ala 35 40 45Gln Asn Ile Ala Asn Gly Thr Ser Phe Leu Lys Asp Pro Ser Lys Ser 50 55 60Lys His Val Thr Gly Asp Ala Ile Asp Phe Ala Pro Tyr Ile Asn Gly65 70 75 80Lys Ile Asp Trp Lys Asp Leu Glu Ala Phe Trp Ala Val Lys Lys Ala 85 90 95Phe Glu Gln Ala Gly Lys Glu Leu Gly Ile Lys Leu Arg Phe Gly Ala 100 105 110Asp Trp Asn Ser Ser Gly Asp Tyr His Asp Glu Ile Asp Arg Gly Thr 115 120 125Tyr Asp Gly Gly His Val Glu Leu Val 130 13529275PRTartificial sequenceFusion of CBD of EL188 endolysin with ABgp46 endolysin 29Met Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val1 5 10 15Lys Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys 20 25 30Gly Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val 35 40 45Val Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala 50 55 60Ser Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu65 70 75 80Gly Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu 85 90 95Ser Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr 100 105 110Pro Thr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala 115 120 125Ala Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu 130 135 140Lys Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu145 150 155 160Gly Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp 165 170 175Met Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Pro Leu 180 185 190Asn Gln Ser Gln Phe Asp Ala Met Ser Asp Leu Val Tyr Asn Ala Gly 195 200 205Val Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys 210 215 220Gly Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln225 230 235 240Asn Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly Arg Val 245 250 255Ala Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile Gly Arg 260 265 270Gly Ala Lys 27530230PRTartificial sequenceFusion of CBD of KZ144 endolysin (three cysteines replaced by serine) with LYS68 endolysin 30Met Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu1 5 10 15Gln Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly 20 25 30Ile Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp 35 40 45Asn Ser Leu Asp Ser Asp Gly Ile Val Gly Lys Asn Thr Trp Ala Glu 50 55 60Leu Phe Ser Lys Tyr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys65 70 75 80Phe Thr Ala Ala Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr 85 90 95Lys Asn Glu Lys Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp 100 105 110Val Lys Glu Gly Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu 115 120 125His Lys Asp Met Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His 130 135 140Pro Pro Leu Asn Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr145 150 155 160Asn Ala Gly Val Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala 165 170 175Leu Arg Lys Gly Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe 180 185 190His Tyr Gln Asn Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala 195 200 205Gly Arg Val Ala Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala 210 215 220Ile Gly Arg Gly Ala Lys225 23031254PRTartificial sequenceFusion of CBD of KZ144 endolysin (three cysteines replaced by serine) with ABgp46 endolysin 31Met Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu1 5 10 15Gln Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly 20 25 30Ile Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp 35 40 45Asn Ser Leu Asp Ser Asp Gly Ile Val Gly Lys Asn Thr Trp Ala Glu 50 55 60Leu Phe Ser Lys Tyr Ser Ile Leu Thr Lys Asp Gly Phe Gly Ile Ile65 70 75 80Arg Asn Glu Leu Phe Gly Gly Lys Leu Asp Gln Thr Gln Val Asp Ala 85 90 95Ile Asn Phe Ile Val Glu Lys Ala Thr Glu Ser Gly Leu Ser Tyr Pro 100 105 110Glu Ala Ala Tyr Leu Leu Ala Thr Ile Tyr His Glu Thr Gly Leu Pro 115 120 125Ser Gly Tyr Arg Thr Met Gln Pro Ile Lys Glu Ala Gly Ser Asp Asn 130 135 140Tyr Leu Arg Ser Lys Lys Tyr Tyr Pro Tyr Ile Gly Tyr Gly Tyr Val145 150 155 160Gln Leu Thr Trp Lys Glu Asn Tyr Gly Arg Ile Gly Lys Leu Ile Gly 165 170 175Ile Asp Leu Ile Lys Asn Pro Glu Lys Ala Leu Glu Pro Leu Ile Ala 180 185 190Ile Gln Ile Ala Ile Lys Gly Met Leu Asn Gly Trp Phe Thr Gly Val 195 200 205Gly Phe Arg Arg Lys Arg Pro Val Ser Lys Tyr Asn Lys Gln Gln Tyr 210 215 220Ile Ala Ala Arg Asn Ile Ile Asn Gly Lys Asp Lys Ala Glu Leu Ile225 230 235 240Ala Lys Tyr Ala Ile Ile Phe Glu Arg Ala Leu Arg Ser Leu 245 25032263PRTArtificial sequenceFusion of CBD of ICP endolysin with ABgp46 endolysin 32Met Ile Leu Lys Arg Gly Ser Ser Gly Ala Asp Val Lys Asn Met Gln1 5 10 15Glu Tyr Leu Thr Ala Leu Gly Tyr Asp Thr Lys Gly Val Glu Gly Thr 20 25 30Phe Glu Gly Gly Thr Glu Ser Ala Val Lys Ala Phe Gln Lys Asp Met 35 40 45Ser Phe Thr Val Val Asp Gly Ile Ile Gly Asn Gln Thr Ala Lys His 50 55 60Leu Val Asp Met Tyr Tyr Gly Lys Val Val Pro Phe Gly Tyr Val Ile65 70 75 80Leu Thr Lys Asp Gly Phe Gly Ile Ile Arg Asn Glu Leu Phe Gly Gly 85 90 95Lys Leu Asp Gln Thr Gln Val Asp Ala Ile Asn Phe Ile Val Glu Lys 100 105 110Ala Thr Glu Ser Gly Leu Ser Tyr Pro Glu Ala Ala Tyr Leu Leu Ala 115 120 125Thr Ile Tyr His Glu Thr Gly Leu Pro Ser Gly Tyr Arg Thr Met Gln 130 135 140Pro Ile Lys Glu Ala Gly Ser Asp Asn Tyr Leu Arg Ser Lys Lys Tyr145 150 155 160Tyr Pro Tyr Ile Gly Tyr Gly Tyr Val Gln Leu Thr Trp Lys Glu Asn 165 170 175Tyr Gly Arg Ile Gly Lys Leu Ile Gly Ile Asp Leu Ile Lys Asn Pro 180 185 190Glu Lys Ala Leu Glu Pro Leu Ile Ala Ile Gln Ile Ala Ile Lys Gly 195 200 205Met Leu Asn Gly Trp Phe Thr Gly Val Gly Phe Arg Arg Lys Arg Pro 210 215 220Val Ser Lys Tyr Asn Lys Gln Gln Tyr Ile Ala Ala Arg Asn Ile Ile225 230 235 240Asn Gly Lys Asp Lys Ala Glu Leu Ile Ala Lys Tyr Ala Ile Ile Phe 245 250 255Glu Arg Ala Leu Arg Ser Leu 26033215PRTartificial sequenceFusion of CBD of ICP endolysin with LYS394 endolysin 33Met Ile Leu Lys Arg Gly Ser Ser Gly Ala Asp Val Lys Asn Met Gln1 5 10 15Glu Tyr Leu Thr Ala Leu Gly Tyr Asp Thr Lys Gly Val Glu Gly Thr 20 25 30Phe Glu Gly Gly Thr Glu Ser Ala Val Lys Ala Phe Gln Lys Asp Met 35 40 45Ser Phe Thr Val Val Asp Gly Ile Ile Gly Asn Gln Thr Ala Lys His 50 55 60Leu Val Asp Met Tyr Tyr Gly Lys Val Val Pro Phe Gly Tyr Val Ser65 70 75 80Phe Lys Phe Gly Lys Asn Ser Glu Lys Gln Leu Ala Thr Val Lys Pro 85 90 95Glu Leu Gln Lys Val Ala Arg Arg Ala Leu Glu Leu Ser Pro Tyr Asp 100 105 110Phe Thr Ile Val Gln Gly Ile Arg Thr Val Ala Gln Ser Ala Gln Asn 115 120 125Ile Ala Asn Gly Thr Ser Phe Leu Lys Asp Pro Ser Lys Ser Lys His 130 135 140Val Thr Gly Asp Ala Ile Asp Phe Ala Pro Tyr Ile Asn Gly Lys Ile145 150 155 160Asp Trp Lys Asp Leu Glu Ala Phe Trp Ala Val Lys Lys Ala Phe Glu 165 170 175Gln Ala Gly Lys Glu Leu Gly Ile Lys Leu Arg Phe Gly Ala Asp Trp 180 185 190Asn Ser Ser Gly Asp Tyr His Asp Glu Ile Asp Arg Gly Thr Tyr Asp 195 200 205Gly Gly His Val Glu Leu Val 210 21534230PRTartificial sequenceFusion of CBD of KZ144 endolysin (three cysteines replaced by serine) with LYS68 endolysin 34Met Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu1 5 10 15Gln Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly 20 25 30Ile Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp 35 40 45Asn Ser Leu Asp Ser Asp Gly Ile Val Gly Met Asn Thr Trp Ala Glu 50 55 60Leu Phe Ser Lys Tyr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys65 70 75 80Phe Thr Ala Ala Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr 85 90 95Lys Asn Glu Lys Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp 100 105 110Val Lys Glu Gly Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu

115 120 125His Lys Asp Met Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His 130 135 140Pro Ser Leu Asn Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr145 150 155 160Asn Ala Gly Val Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala 165 170 175Leu Arg Lys Gly Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe 180 185 190His Tyr Gln Asn Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala 195 200 205Gly Arg Val Ala Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala 210 215 220Ile Gly Arg Gly Ala Lys225 23035274PRTartificial sequenceFusion of CBD of EL188 endolysin with ABgp46 endolysin w/o starting methionine 35Asn Phe Arg Thr Lys Asn Gly Tyr Arg Asp Leu Gln Ala Leu Val Lys1 5 10 15Glu Leu Gly Leu Tyr Thr Gly Gln Ile Asp Gly Val Trp Gly Lys Gly 20 25 30Thr Ser Ser Ser Thr Glu Thr Leu Leu Arg Gly Tyr Ala Glu Val Val 35 40 45Gly Lys Asn Thr Gly Gly Ile Gly Leu Pro Thr Thr Ser Asp Ala Ser 50 55 60Gly Tyr Asn Val Ile Thr Ala Leu Gln Arg Asn Leu Ala Phe Leu Gly65 70 75 80Leu Tyr Ser Leu Thr Val Asp Gly Ile Trp Gly Asn Gly Thr Leu Ser 85 90 95Gly Leu Asp Lys Ala Phe Glu Val Tyr Lys Glu Arg Tyr Arg Thr Pro 100 105 110Thr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe Thr Ala Ala 115 120 125Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys Asn Glu Lys 130 135 140Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val Lys Glu Gly145 150 155 160Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His Lys Asp Met 165 170 175Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro Pro Leu Asn 180 185 190Gln Ser Gln Phe Asp Ala Met Ser Asp Leu Val Tyr Asn Ala Gly Val 195 200 205Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu Arg Lys Gly 210 215 220Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His Tyr Gln Asn225 230 235 240Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly Arg Val Ala 245 250 255Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile Gly Arg Gly 260 265 270Ala Lys36229PRTartificial sequenceFusion of CBD of KZ144 endolysin (three cysteines replaced by serine) with LYS68 endolysin w/o starting methionine 36Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu Gln1 5 10 15Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 20 25 30Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn 35 40 45Ser Leu Asp Ser Asp Gly Ile Val Gly Lys Asn Thr Trp Ala Glu Leu 50 55 60Phe Ser Lys Tyr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe65 70 75 80Thr Ala Ala Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys 85 90 95Asn Glu Lys Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val 100 105 110Lys Glu Gly Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His 115 120 125Lys Asp Met Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro 130 135 140Pro Leu Asn Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn145 150 155 160Ala Gly Val Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu 165 170 175Arg Lys Gly Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His 180 185 190Tyr Gln Asn Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly 195 200 205Arg Val Ala Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile 210 215 220Gly Arg Gly Ala Lys22537253PRTartificial sequenceFusion of CBD of KZ144 endolysin (three cysteines replaced by serine) with ABgp46 endolysin w/o starting methionine 37Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu Gln1 5 10 15Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 20 25 30Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn 35 40 45Ser Leu Asp Ser Asp Gly Ile Val Gly Lys Asn Thr Trp Ala Glu Leu 50 55 60Phe Ser Lys Tyr Ser Ile Leu Thr Lys Asp Gly Phe Gly Ile Ile Arg65 70 75 80Asn Glu Leu Phe Gly Gly Lys Leu Asp Gln Thr Gln Val Asp Ala Ile 85 90 95Asn Phe Ile Val Glu Lys Ala Thr Glu Ser Gly Leu Ser Tyr Pro Glu 100 105 110Ala Ala Tyr Leu Leu Ala Thr Ile Tyr His Glu Thr Gly Leu Pro Ser 115 120 125Gly Tyr Arg Thr Met Gln Pro Ile Lys Glu Ala Gly Ser Asp Asn Tyr 130 135 140Leu Arg Ser Lys Lys Tyr Tyr Pro Tyr Ile Gly Tyr Gly Tyr Val Gln145 150 155 160Leu Thr Trp Lys Glu Asn Tyr Gly Arg Ile Gly Lys Leu Ile Gly Ile 165 170 175Asp Leu Ile Lys Asn Pro Glu Lys Ala Leu Glu Pro Leu Ile Ala Ile 180 185 190Gln Ile Ala Ile Lys Gly Met Leu Asn Gly Trp Phe Thr Gly Val Gly 195 200 205Phe Arg Arg Lys Arg Pro Val Ser Lys Tyr Asn Lys Gln Gln Tyr Ile 210 215 220Ala Ala Arg Asn Ile Ile Asn Gly Lys Asp Lys Ala Glu Leu Ile Ala225 230 235 240Lys Tyr Ala Ile Ile Phe Glu Arg Ala Leu Arg Ser Leu 245 25038262PRTArtificial sequenceFusion of CBD of ICP endolysin with ABgp46 endolysin w/o starting methionine 38Ile Leu Lys Arg Gly Ser Ser Gly Ala Asp Val Lys Asn Met Gln Glu1 5 10 15Tyr Leu Thr Ala Leu Gly Tyr Asp Thr Lys Gly Val Glu Gly Thr Phe 20 25 30Glu Gly Gly Thr Glu Ser Ala Val Lys Ala Phe Gln Lys Asp Met Ser 35 40 45Phe Thr Val Val Asp Gly Ile Ile Gly Asn Gln Thr Ala Lys His Leu 50 55 60Val Asp Met Tyr Tyr Gly Lys Val Val Pro Phe Gly Tyr Val Ile Leu65 70 75 80Thr Lys Asp Gly Phe Gly Ile Ile Arg Asn Glu Leu Phe Gly Gly Lys 85 90 95Leu Asp Gln Thr Gln Val Asp Ala Ile Asn Phe Ile Val Glu Lys Ala 100 105 110Thr Glu Ser Gly Leu Ser Tyr Pro Glu Ala Ala Tyr Leu Leu Ala Thr 115 120 125Ile Tyr His Glu Thr Gly Leu Pro Ser Gly Tyr Arg Thr Met Gln Pro 130 135 140Ile Lys Glu Ala Gly Ser Asp Asn Tyr Leu Arg Ser Lys Lys Tyr Tyr145 150 155 160Pro Tyr Ile Gly Tyr Gly Tyr Val Gln Leu Thr Trp Lys Glu Asn Tyr 165 170 175Gly Arg Ile Gly Lys Leu Ile Gly Ile Asp Leu Ile Lys Asn Pro Glu 180 185 190Lys Ala Leu Glu Pro Leu Ile Ala Ile Gln Ile Ala Ile Lys Gly Met 195 200 205Leu Asn Gly Trp Phe Thr Gly Val Gly Phe Arg Arg Lys Arg Pro Val 210 215 220Ser Lys Tyr Asn Lys Gln Gln Tyr Ile Ala Ala Arg Asn Ile Ile Asn225 230 235 240Gly Lys Asp Lys Ala Glu Leu Ile Ala Lys Tyr Ala Ile Ile Phe Glu 245 250 255Arg Ala Leu Arg Ser Leu 26039214PRTartificial sequenceFusion of CBD of ICP endolysin with LYS394 endolysin w/o starting methionine 39Ile Leu Lys Arg Gly Ser Ser Gly Ala Asp Val Lys Asn Met Gln Glu1 5 10 15Tyr Leu Thr Ala Leu Gly Tyr Asp Thr Lys Gly Val Glu Gly Thr Phe 20 25 30Glu Gly Gly Thr Glu Ser Ala Val Lys Ala Phe Gln Lys Asp Met Ser 35 40 45Phe Thr Val Val Asp Gly Ile Ile Gly Asn Gln Thr Ala Lys His Leu 50 55 60Val Asp Met Tyr Tyr Gly Lys Val Val Pro Phe Gly Tyr Val Ser Phe65 70 75 80Lys Phe Gly Lys Asn Ser Glu Lys Gln Leu Ala Thr Val Lys Pro Glu 85 90 95Leu Gln Lys Val Ala Arg Arg Ala Leu Glu Leu Ser Pro Tyr Asp Phe 100 105 110Thr Ile Val Gln Gly Ile Arg Thr Val Ala Gln Ser Ala Gln Asn Ile 115 120 125Ala Asn Gly Thr Ser Phe Leu Lys Asp Pro Ser Lys Ser Lys His Val 130 135 140Thr Gly Asp Ala Ile Asp Phe Ala Pro Tyr Ile Asn Gly Lys Ile Asp145 150 155 160Trp Lys Asp Leu Glu Ala Phe Trp Ala Val Lys Lys Ala Phe Glu Gln 165 170 175Ala Gly Lys Glu Leu Gly Ile Lys Leu Arg Phe Gly Ala Asp Trp Asn 180 185 190Ser Ser Gly Asp Tyr His Asp Glu Ile Asp Arg Gly Thr Tyr Asp Gly 195 200 205Gly His Val Glu Leu Val 21040229PRTartificial sequenceFusion of CBD of KZ144 endolysin (three cysteines replaced by serine) with LYS68 endolysin w/o starting methionine 40Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu Gln1 5 10 15Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 20 25 30Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn 35 40 45Ser Leu Asp Ser Asp Gly Ile Val Gly Met Asn Thr Trp Ala Glu Leu 50 55 60Phe Ser Lys Tyr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe65 70 75 80Thr Ala Ala Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys 85 90 95Asn Glu Lys Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val 100 105 110Lys Glu Gly Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His 115 120 125Lys Asp Met Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro 130 135 140Ser Leu Asn Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn145 150 155 160Ala Gly Val Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu 165 170 175Arg Lys Gly Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His 180 185 190Tyr Gln Asn Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly 195 200 205Arg Val Ala Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile 210 215 220Gly Arg Gly Ala Lys225416PRTartificialsynthetic sequence 41Lys Arg Lys Lys Arg Lys1 5425PRTartificialsynethtic sequencemisc_feature(3)..(3)Xaa can be any naturally occurring amino acid 42Lys Arg Xaa Lys Arg1 5435PRTartificialsynthetic sequence 43Lys Arg Ser Lys Arg1 5445PRTartificialsynthetic sequence 44Lys Arg Gly Ser Gly1 5459PRTartificialsynthetic sequence 45Lys Arg Lys Lys Arg Lys Lys Arg Lys1 5469PRTartificialsynthetic sequence 46Arg Arg Arg Arg Arg Arg Arg Arg Arg1 5478PRTartificialsynthetic sequence 47Lys Lys Lys Lys Lys Lys Lys Lys1 54810PRTartificialsynthetic sequence 48Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys1 5 104912PRTartificialsynthetic sequence 49Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys1 5 105014PRTartificialsynthetic sequence 50Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg1 5 105116PRTartificialsynthetic sequence 51Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys1 5 10 155218PRTartificialsynthetic sequence 52Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys1 5 10 15Arg Lys5319PRTartificialsynthetic sequence 53Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys1 5 10 15Arg Lys Lys5419PRTartificialsynthetic sequence 54Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg Arg1 5 10 15Arg Arg Arg5519PRTartificialsynthetic sequence 55Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys Lys1 5 10 15Lys Lys Lys5620PRTartificialsynthetic sequence 56Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg1 5 10 15Lys Lys Arg Lys 205721PRTartificialsynthetic sequence 57Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg1 5 10 15Lys Lys Arg Lys Lys 205821PRTartificialsynthetic sequence 58Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys1 5 10 15Arg Lys Lys Arg Lys 205922PRTartificialsynthetic sequence 59Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Lys Arg Lys1 5 10 15Lys Arg Lys Lys Arg Lys 206024PRTartificialsynthetic sequence 60Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Ser Gly Lys1 5 10 15Arg Lys Lys Arg Lys Lys Arg Lys 206125PRTartificialsynthetic sequence 61Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys1 5 10 15Arg Lys Lys Arg Lys Lys Arg Lys Lys 20 256231PRTartificialsynthetic sequence 62Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg1 5 10 15Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys 20 25 306338PRTartificialsynthetic sequence 63Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Gly Ser Gly Ser Gly Lys1 5 10 15Arg Lys Lys Arg Lys Lys Arg Lys Gly Ser Gly Ser Gly Lys Arg Lys 20 25 30Lys Arg Lys Lys Arg Lys 356439PRTartificialsynthetic sequence 64Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys1 5 10 15Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg Lys Lys Arg 20 25 30Lys Lys Arg Lys Lys Arg Lys 356542PRTartificialsynthetic sequence 65Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg1 5 10 15Lys Lys Arg Lys Arg Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys Arg 20 25 30Ser Lys Arg Lys Lys Arg Lys Lys Arg Lys 35 406637PRTHomo sapiens 66Leu Leu Gly Asp Phe Phe Arg Lys Ser Lys Glu Lys Ile Gly Lys Glu1 5 10 15Phe Lys Arg Ile Val Gln Arg Ile Lys Asp Phe Leu Arg Asn Leu Val 20 25 30Pro Arg Thr Glu Ser 356729PRTunknownSMAP-29 sheep 67Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys Lys1 5 10 15Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly 20 256813PRTunknownIndolicidine bovine 68Ile Leu Pro Trp Lys Trp Pro Trp Trp Pro Trp Arg Arg1 5 106918PRTunknownProtegrin Porcine 69Arg Gly Gly Arg Leu Cys Tyr Cys Arg Arg Arg Phe Cys Val Cys Val1 5 10 15Gly Arg7031PRTunknownCecropin P1 Mammal (pig) 70Ser Trp Leu Ser Lys Thr Ala Lys Lys Leu Glu Asn Ser Ala Lys Lys1 5 10 15Arg Ile Ser Glu Gly Ile Ala Ile Ala Ile Gln Gly Gly Pro Arg 20 25 307123PRTunknownMagainin frog 71Gly Ile Gly Lys Phe Leu His Ser Ala Lys Lys Phe Gly Lys Ala Phe1 5 10 15Val Gly Glu Ile Met Asn Ser 207225PRTunknownPleurocidin fish 72Gly Trp Gly Ser Phe Phe Lys Lys Ala Ala His Val Gly Lys His Val1 5 10 15Gly Lys Ala Ala Leu Thr His Tyr Leu 20 257336PRTAedes aegypti 73Gly Gly Leu Lys Lys Leu Gly Lys Lys Leu Glu

Gly Ala Gly Lys Arg1 5 10 15Val Phe Asn Ala Ala Glu Lys Ala Leu Pro Val Val Ala Gly Ala Lys 20 25 30Ala Leu Arg Lys 357440PRTDrosophila melanogaster 74Gly Trp Leu Lys Lys Ile Gly Lys Lys Ile Glu Arg Val Gly Gln His1 5 10 15Thr Arg Asp Ala Thr Ile Gln Gly Leu Gly Ile Pro Gln Gln Ala Ala 20 25 30Asn Val Ala Ala Thr Ala Arg Gly 35 407521PRTunknownBuforin II vertebrate 75Thr Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His1 5 10 15Arg Leu Leu Arg Lys 207639PRTunknownSarcotoxin IA Fly 76Gly Trp Leu Lys Lys Ile Gly Lys Lys Ile Glu Arg Val Gly Gln His1 5 10 15Thr Arg Asp Ala Thr Ile Gln Gly Leu Gly Ile Ala Gln Gln Ala Ala 20 25 30Asn Val Ala Ala Thr Ala Arg 357717PRTApis mellifera 77Ala Asn Arg Pro Val Tyr Ile Pro Pro Pro Arg Pro Pro His Pro Arg1 5 10 15Leu7824PRTunknownAscaphine 5 Frog 78Gly Ile Lys Asp Trp Ile Lys Gly Ala Ala Lys Lys Leu Ile Lys Thr1 5 10 15Val Ala Ser His Ile Ala Asn Gln 207922PRTunknownNigrocine 2 Frog 79Gly Leu Leu Ser Lys Val Leu Gly Val Gly Lys Lys Val Leu Cys Gly1 5 10 15Val Ser Gly Leu Val Cys 208024PRTunknownPseudin 1 Rana Frog 80Gly Leu Asn Thr Leu Lys Lys Val Phe Gln Gly Leu His Glu Ala Ile1 5 10 15Lys Leu Ile Asn Asn His Val Gln 208118PRTunknownRanalexin Frog 81Phe Leu Gly Gly Leu Ile Val Pro Ala Met Ile Cys Ala Val Thr Lys1 5 10 15Lys Cys8226PRTunknownMelittin bee 82Gly Ile Gly Ala Val Leu Lys Val Leu Thr Thr Gly Leu Pro Ala Leu1 5 10 15Ile Ser Trp Ile Lys Arg Lys Arg Gln Gln 20 258325PRTunknownLycotoxin 1 Spider 83Ile Trp Leu Thr Ala Leu Lys Phe Leu Gly Lys His Ala Ala Lys Lys1 5 10 15Leu Ala Lys Gln Gln Leu Ser Lys Leu 20 258419PRTunknownParasin 1 Fish 84Lys Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Ala Lys Ala Lys Thr1 5 10 15Arg Ser Ser8539PRTunknownBuforin I Toad 85Ala Gly Arg Gly Lys Gln Gly Gly Lys Val Arg Ala Lys Ala Lys Thr1 5 10 15Arg Ser Ser Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Val His Arg 20 25 30Leu Leu Arg Lys Gly Asn Tyr 358634PRTunknownDermaseptin 1 Frog 86Ala Leu Trp Lys Thr Met Leu Lys Lys Leu Gly Thr Met Ala Leu His1 5 10 15Ala Gly Lys Ala Ala Leu Gly Ala Ala Ala Asp Thr Ile Ser Gln Gly 20 25 30Thr Gln8712PRTunknownBactenecin 1 Cow 87Arg Leu Cys Arg Ile Val Val Ile Arg Val Cys Arg1 5 108821PRTunknownThanatin Insect 88Gly Ser Lys Lys Pro Val Pro Ile Ile Tyr Cys Asn Arg Arg Thr Gly1 5 10 15Lys Cys Gln Arg Met 208919PRTunknownBrevinin 1T Rana frogs 89Val Asn Pro Ile Ile Leu Gly Val Leu Pro Lys Val Cys Leu Ile Thr1 5 10 15Lys Lys Cys9026PRTunknownRanateurin 1 Rana frog 90Ser Met Leu Ser Val Leu Lys Asn Leu Gly Lys Val Gly Leu Gly Phe1 5 10 15Val Ala Cys Lys Ile Asn Ile Lys Gln Cys 20 259146PRTunknownEsculentin 1 Rana frogs 91Gly Ile Phe Ser Lys Leu Gly Arg Lys Lys Ile Lys Asn Leu Leu Ile1 5 10 15Ser Gly Leu Lys Asn Val Gly Lys Glu Val Gly Met Asp Val Val Arg 20 25 30Thr Gly Ile Lys Ile Ala Gly Cys Lys Ile Lys Gly Glu Cys 35 40 459217PRTLimulus polyphemus 92Arg Trp Cys Phe Arg Val Cys Tyr Arg Gly Ile Cys Tyr Arg Lys Cys1 5 10 15Arg9325PRTunknownAndroctonin Scorpion 93Arg Ser Val Cys Arg Gln Ile Lys Ile Cys Arg Arg Arg Gly Gly Cys1 5 10 15Tyr Tyr Lys Cys Thr Asn Arg Pro Tyr 20 259430PRTHomo sapiens 94Asp Cys Tyr Cys Arg Ile Pro Ala Cys Ile Ala Gly Glu Arg Arg Tyr1 5 10 15Gly Thr Cys Ile Tyr Gln Gly Arg Leu Trp Ala Phe Cys Cys 20 25 309538PRTunknownbeta-defensin cow 95Asn Pro Val Ser Cys Val Arg Asn Lys Gly Ile Cys Val Pro Ile Arg1 5 10 15Cys Pro Gly Ser Met Lys Gln Ile Gly Thr Cys Val Gly Arg Ala Val 20 25 30Lys Cys Cys Arg Lys Lys 359618PRTunknowntheta-defensin monkey 96Gly Phe Cys Arg Cys Leu Cys Arg Arg Gly Val Cys Arg Cys Ile Cys1 5 10 15Thr Arg9740PRTunknowndefensin (sapecin A) insect 97Ala Thr Cys Asp Leu Leu Ser Gly Thr Gly Ile Asn His Ser Ala Cys1 5 10 15Ala Ala His Cys Leu Leu Arg Gly Asn Arg Gly Gly Tyr Cys Asn Gly 20 25 30Lys Ala Val Cys Val Cys Arg Asn 35 409846PRTunknownThionin (crambin) plant 98Thr Thr Cys Cys Pro Ser Ile Val Ala Arg Ser Asn Phe Asn Val Cys1 5 10 15Arg Ile Pro Gly Thr Pro Glu Ala Ile Cys Ala Thr Tyr Thr Gly Cys 20 25 30Ile Ile Ile Pro Gly Ala Thr Cys Pro Gly Asp Tyr Ala Asn 35 40 459950PRTunknowndefensin from radish 99Gln Lys Leu Cys Gln Arg Pro Ser Gly Thr Trp Ser Gly Val Cys Gly1 5 10 15Asn Asn Asn Ala Cys Lys Asn Gln Cys Ile Arg Leu Glu Lys Ala Arg 20 25 30His Gly Ser Cys Asn Tyr Val Phe Pro Ala His Cys Ile Cys Tyr Phe 35 40 45Pro Cys 5010044PRTDrosophila melanogaster 100Asp Cys Leu Ser Gly Arg Tyr Lys Gly Pro Cys Ala Val Trp Asp Asn1 5 10 15Glu Thr Cys Arg Arg Val Cys Lys Glu Glu Gly Arg Ser Ser Gly His 20 25 30Cys Ser Pro Ser Leu Lys Cys Trp Cys Glu Gly Cys 35 4010125PRTHomo sapiens 101Asp Thr His Phe Pro Ile Cys Ile Phe Cys Cys Gly Cys Cys His Arg1 5 10 15Ser Lys Cys Gly Met Cys Cys Lys Thr 20 2510244PRTunknownBac 5 Cow 102Arg Phe Arg Pro Pro Ile Arg Arg Pro Pro Ile Arg Pro Pro Phe Tyr1 5 10 15Pro Pro Phe Arg Pro Pro Ile Arg Pro Pro Ile Phe Pro Pro Ile Arg 20 25 30Pro Pro Phe Arg Pro Pro Leu Gly Arg Pro Phe Pro 35 4010339PRTunknownPR-39 Pig 103Arg Arg Arg Pro Arg Pro Pro Tyr Leu Pro Arg Pro Arg Pro Pro Pro1 5 10 15Phe Phe Pro Pro Arg Leu Pro Pro Arg Ile Pro Pro Gly Phe Pro Pro 20 25 30Arg Phe Pro Pro Arg Phe Pro 3510420PRTunknownPyrrhocoricin Insect 104Val Asp Lys Gly Ser Tyr Leu Pro Arg Pro Thr Pro Pro Arg Pro Ile1 5 10 15Tyr Asn Arg Asn 2010524PRTHomo sapiens 105Asp Ser His Ala Lys Arg His His Gly Tyr Lys Arg Lys Phe His Glu1 5 10 15Lys His His Ser His Arg Gly Tyr 2010619PRTUnknownECP19 106Arg Pro Pro Gln Phe Thr Arg Ala Gln Trp Phe Ala Ile Gln His Ile1 5 10 15Ser Leu Asn10723PRTUnknownMSI-594 107Gly Ile Gly Lys Phe Leu Lys Lys Ala Lys Lys Gly Ile Gly Ala Val1 5 10 15Leu Lys Val Leu Thr Thr Gly 2010835PRTUnknownTL-ColM 108Met Glu Thr Leu Thr Val His Ala Pro Ser Pro Ser Thr Asn Leu Pro1 5 10 15Ser Tyr Gly Asn Gly Ala Phe Ser Leu Ser Ala Pro His Val Pro Gly 20 25 30Ala Gly Pro 3510918PRTUnknownSBO 109Lys Leu Lys Lys Ile Ala Gln Lys Ile Lys Asn Phe Phe Ala Lys Leu1 5 10 15Val Ala11034PRTLimulus polyphemus 110Gly Phe Lys Leu Lys Gly Met Ala Arg Ile Ser Cys Leu Pro Asn Gly1 5 10 15Gln Trp Ser Asn Phe Pro Pro Lys Cys Ile Arg Glu Cys Ala Met Val 20 25 30Ser Ser11118PRTartificialsynthetic sequence 111Gly Phe Phe Ile Pro Ala Val Ile Leu Pro Ser Ile Ala Phe Leu Ile1 5 10 15Val Pro1125PRTartificialsynthetic sequence 112Phe Phe Val Ala Pro1 511313PRTunknownalpha4-helix of T4 lysozyme 113Pro Asn Arg Ala Lys Arg Val Ile Thr Thr Phe Arg Thr1 5 1011427PRTartificialsynthetic sequence 114Lys Arg Trp Val Lys Arg Val Lys Arg Val Lys Arg Trp Val Lys Arg1 5 10 15Val Val Arg Val Val Lys Arg Trp Val Lys Arg 20 2511525PRTArtificial Sequencesynthetic sequence; MW2 115Gly Lys Pro Gly Trp Leu Ile Lys Val Ala Leu Lys Phe Lys Lys Leu1 5 10 15Ile Arg Arg Pro Leu Lys Arg Leu Ala 20 25116261PRTArtificial sequenceFusion of SMAP-29, CBD of KZ144 endolysin (three cysteines replaced by serine, K59M) with LYS68 endolysin (P78S) 116Met Arg Gly Leu Arg Arg Leu Gly Arg Lys Ile Ala His Gly Val Lys1 5 10 15Lys Tyr Gly Pro Thr Val Leu Arg Ile Ile Arg Ile Ala Gly Gly Ser 20 25 30Lys Val Leu Arg Lys Gly Asp Arg Gly Asp Glu Val Ser Gln Leu Gln 35 40 45Thr Leu Leu Asn Leu Ser Gly Tyr Asp Val Gly Lys Pro Asp Gly Ile 50 55 60Phe Gly Asn Asn Thr Phe Asn Gln Val Val Lys Phe Gln Lys Asp Asn65 70 75 80Ser Leu Asp Ser Asp Gly Ile Val Gly Met Asn Thr Trp Ala Glu Leu 85 90 95Phe Ser Lys Tyr Ser Asn Arg Asn Ile Ser Asp Asn Gly Ile Lys Phe 100 105 110Thr Ala Ala Phe Glu Gly Phe Arg Gly Thr Ala Tyr Arg Ala Thr Lys 115 120 125Asn Glu Lys Tyr Leu Thr Ile Gly Tyr Gly His Tyr Gly Ala Asp Val 130 135 140Lys Glu Gly Gln Lys Ile Thr Glu Gly Gln Gly Leu Leu Leu Leu His145 150 155 160Lys Asp Met Val Lys Ala Val Ala Ala Val Asp Ala Val Ala His Pro 165 170 175Ser Leu Asn Gln Ser Gln Phe Asp Ala Met Cys Asp Leu Val Tyr Asn 180 185 190Ala Gly Val Gly Val Ile Ala Ala Ser Thr Gly Thr Gly Gln Ala Leu 195 200 205Arg Lys Gly Asp Val Ala Thr Leu Arg Asn Lys Leu Thr Gln Phe His 210 215 220Tyr Gln Asn Gly Lys Ser Leu Leu Gly Leu Arg Arg Arg Ala Ala Gly225 230 235 240Arg Val Ala Leu Phe Asp Gly Met Leu Trp Gln Gln Ala Glu Ala Ile 245 250 255Gly Arg Gly Ala Lys 2601174PRTartificiallinker 117Gly Ala Gly Ala11188PRTartificiallinker 118Gly Ala Gly Ala Gly Ala Gly Ala1 511912PRTartificiallinker 119Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala Gly Ala1 5 101206PRTartificial sequenceHis-Tag (6x) 120His His His His His His1 5

Claims

1. A polypeptide comprising the amino acid sequence of a globular Gram-negative endolysin and the amino acid sequence of a cell wall binding domain of i) a modular Gram-negative endolysin or ii) a bacteriophage tail/baseplate protein.

2. The polypeptide according to claim 1, wherein the Gram-negative modular endolysin is selected from the group consisting of KZ144, EL188, OBPgpLYS, PVPSE1gp146, and 201.phi.2-1 endolysin.

3. The polypeptide according to claim 1, wherein the bacteriophage tail/baseplate protein is a bacteriophage tail/baseplate protein of a bacteriophage selected from the group consisting of Vibrio phage ICP1 and Vibrio phage RYC.

4. The polypeptide according to claim 1, wherein the polypeptide comprises an amino acid sequence selected from the group of sequences consisting of SEQ ID Nos.: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 and 14, and sequences having at least 80% sequence identity to any of these SEQ ID Nos.

5. The polypeptide according to any one of the preceding claims, wherein the globular endolysin is a endopeptidase, chitinase, T4 like muraminidase, lambda like muraminidase, N-acetyl-muramoyl-L-alanine-amidase, muramoyl-L-alanine-amidase, muramidase, lytic transglycosylase (C), lytic transglycosylase (M), N-acetyl-muramidase, N-acetyl-glucosaminidase or transglycosylase.

6. The polypeptide according to any one of the preceding claims, wherein the globular endolysin is an endolysin deriving from a bacteriophage infecting bacteria of the genus selected from the group consisting of: Acinetobacter, Aeromonas, Aggregatibacter, Azospirillum, Bacteroides, Burkholderia, Campylobacter, Candidatus, Caulobacter, Clavibacter, Cronobacter, Delftia, Enterobacter, Erwinia, Escherichia, Flavobacterium, Haemophilus, Iodobacteria, Klebsiella, Kluyvera, Mannheimia, Morganella, Neisseria, Pantoea, Pasteurella, Planktothrix, Pseudoalteromonas, Pseudomonas, Ralstonia, Salmonella, Shigella, Sinorhizobium, Sodalis, Synechococcus, Thalassomonas, Thermus, Vibrio, Xanthomonas, Xylella, and Yersinia.

7. The polypeptide according to any one of the preceding claims, wherein the globular endolysin is deriving from the group of endolysins listed in table 1.

8. The polypeptide according to claim 7, wherein the globular endolysin is selected from the group consisting of Lys68, ABgp46 and Lys394 endolysin, in particular wherein the globular endolysin is Lys68 endolysin.

9. The polypeptide according to any one of the preceding claims, wherein the polypeptide comprises an amino acid sequence selected from the group of sequences consisting of SEQ ID Nos.: 18, 19, 20, 21, 22, 23, 24, 25, 27 and 28.

10. The polypeptide according to any one of the preceding claims, wherein the polypeptide does not comprise the amino acid sequence of a Gram-negative modular endolysin.

11. The polypeptide according to any one of the preceding claims, wherein the polypeptide does not comprise an enzymatically active domain (EAD) of a Gram-negative modular endolysin.

12. The polypeptide according to any one of the preceding claims, wherein the enzymatic activity of the globular endolysin is the only enzymatic activity of the polypeptide.

13. The polypeptide according to any one of the preceding claims, wherein the amino acid sequence of the globular endolysin exhibits less than 90% sequence identity with the amino acid sequence of an enzymatically active domain of any modular endolysin.

14. The polypeptide according to any one of the preceding claims, wherein the amino acid sequence of the globular endolysin and the amino acid sequence of the cell wall binding domain are either linked directly to each other or via an intermediate linker sequence, the linker sequence preferably not exceeding more than 50 amino acids in length.

15. The polypeptide according to any one of the preceding claims, wherein the polypeptide degrades peptidoglycan of at least one Gram-negative bacterial species.

16. The polypeptide according to any one of the preceding claims, wherein the polypeptide binds to peptidoglycan of at least one Gram-negative bacterial species.

17. The polypeptide according to any one of the preceding claims wherein the polypeptide comprises an amino acid sequence selected from the group of sequences consisting of SEQ ID NOs: 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 and 40, wherein SEQ ID NO: 40 is preferred.

18. The polypeptide according to any one of the preceding claims, wherein the polypeptide comprises additionally at least one amino acid sequence selected from the group consisting of amphipathic peptide, cationic peptide, polycationic peptide, hydrophobic peptide, naturally occurring antimicrobial peptide, sushi peptide and defensin.

19. The polypeptide according to claim 18, wherein the additional amino acid sequence is present at the N- or C-terminus of the polypeptide.

20. The polypeptide according to claim 18, wherein the polypeptide comprises at least one additional amino acid sequence stretch selected from the group consisting of: KRK and SEQ ID NOs: 41-115.

21. The polypeptide according to claim 18, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 116.

22. The polypeptide according to any one of the preceding claims, wherein the polypeptide comprises additionally a tag sequence.

23. Nucleic acid encoding a polypeptide according to any one of claims 1 to 22.

24. Vector comprising a nucleic acid according to claim 23.

25. Host cell comprising a polypeptide according to any one of claims 1 to 22, a nucleic acid according to claim 23, and/or a vector according to claim 24.

26. Composition comprising a polypeptide according to any one of claims 1 to 22, a nucleic acid according to claim 23, a vector according to claim 24 and/or a host cell according to claim 25.

27. Composition according to claim 26, wherein the composition is a pharmaceutical composition comprising a pharmaceutical acceptable diluent, excipient or carrier.

28. The polypeptide according to any one of claims 1 to 22, the nucleic acid according to claim 23, the vector according to claim 24, the host cell according to claim 25 and/or the composition according to claim 26 or 27 for use in a method for treatment of the human or animal body by surgery or therapy or for use in diagnostic methods practised on the human or animal body.

29. The polypeptide, nucleic acid, vector, host cell, or composition for use according to claim 28, wherein the method is a method for preventing or treating bacterial infections of the human or animal body.

30. The polypeptide, nucleic acid, vector, host cell, or composition for use according to claim 29, wherein the method is a method for preventing or treating bacterial infections caused by Gram negative bacteria.

31. Use of polypeptide according to any one of claims 1 to 22 and/or the composition according to claim 26 or 27 as non-therapeutic disinfectant.

32. A method for treatment of the human or animal body by surgery or therapy, wherein the method comprises administering an efficient amount of the polypeptide according to any one of claims 1 to 22, the nucleic acid according to claim 23, the vector according to claim 24, the host cell according to claim 25 and/or the composition according to claim 26 or 27.

33. The method according to claim 32, wherein the method is a method for preventing or treating bacterial infections of the human or animal body, in particular wherein the method is a method for preventing or treating bacterial infections caused by Gram negative bacteria.