SINGLE-CHAIN CD137-RECEPTOR AGONIST PROTEINS

Abstract

Provided herein are specific CD137 receptor agonist proteins, nucleic acids encoding the same, and methods of treating a subject having a CD137L-associated disease or disorder. The CD137 receptor agonist proteins provided herein comprise three soluble CD137L domains and an Fe fragment. The CD137 receptor agonist proteins are substantially non-aggregating and suitable for therapeutic, diagnostic and/or research applications.

Description

[0001] This application is a continuation of U.S. patent application Ser. No. 15/955,086, filed Apr. 17, 2018; which is a continuation of PCT/EP2016/075543, filed Oct. 24, 2016; which claims priority to U.S. Provisional Application No. 62/245,838, filed Oct. 23, 2015. The contents of the above applications are incorporated herein by reference in their entirety.

REFERENCE TO SEQUENCE LISTING, TABLE OR COMPUTER PROGRAM

[0002] The Sequence Listing is concurrently submitted herewith with the specification as an ASCII formatted text file via EFS-Web with a file name of Sequence_Listing.txt with a creation date of Apr. 11, 2018, and a size of 109 kilobytes. The Sequence Listing filed via EFS-Web is part of the specification and is hereby incorporated in its entirety by reference herein.

FIELD OF THE INVENTION

[0003] The present invention provides specific CD137 receptor agonist proteins comprising three soluble CD137L domains and an Fc fragment, nucleic acid molecules encoding the CD137 receptor agonist proteins, and uses thereof. The CD137 receptor agonist proteins are substantially non-aggregating and suitable for therapeutic, diagnostic and/or research applications.

BACKGROUND OF THE INVENTION

[0004] It is known that trimerization of TNF superfamily (TNFSF) cytokines is required for efficient receptor binding and activation. Trimeric complexes of TNF superfamily cytokines, however, are difficult to prepare from recombinant monomeric units.

[0005] WO 01/49866 and WO 02/09055 disclose recombinant fusion proteins comprising a TNF cytokine and a multimerization component, particularly a protein from the C1q protein family or a collectin. A disadvantage of these fusion proteins is, however, that the trimerization domain usually has a large molecular weight and/or that the trimerization is rather inefficient.

[0006] Schneider et al. (J Exp Med 187 (1989), 1205-1213) describe that trimers of TNF cytokines are stabilized by N-terminally positioned stabilization motifs. In CD95L, the stabilization of the receptor binding domain trimer is presumably caused by N-terminal amino acid domains which are located near the cytoplasmic membrane.

[0007] Shiraishi et al. (Biochem Biophys Res Commun 322 (2004), 197-202) describe that the receptor binding domain of CD95L may be stabilized by N-terminally positioned artificial .alpha.-helical coiled-coil (leucine zipper) motifs. It was found, however, that the orientation of the polypeptide chains to each other, e.g. parallel or antiparallel orientation, can hardly be predicted. Further, the optimal number of heptad-repeats in the coiled-coil zipper motif are difficult to determine. In addition, coiled-coil structures have the tendency to form macromolecular aggregates after alteration of pH and/or ionic strength.

[0008] WO 01/25277 relates to single-chain oligomeric polypeptides which bind to an extracellular ligand binding domain of a cellular receptor, wherein the polypeptide comprises at least three receptor binding sites of which at least one is capable of binding to a ligand binding domain of the cellular receptor and at least one is incapable of effectively binding to a ligand binding domain of the cellular receptor, whereby the single-chain oligomeric polypeptides are capable of binding to the receptor, but incapable of activating the receptor. For example, the monomers are derived from cytokine ligands of the TNF family, particularly from TNF-.alpha..

[0009] WO 2005/103077 discloses single-chain fusion polypeptides comprising at least three monomers of a TNF family ligand member and at least two peptide linkers that link the monomers of the TNF ligand family members to one another. Recent experiments, however, have shown that these single chain fusion polypeptides show undesired aggregation.

[0010] WO 2010/010051 discloses single-chain fusion polypeptides comprising three soluble TNF family cytokine domains and at least two peptide linkers. The described fusion polypeptides are substantially non-aggregating.

[0011] Recent studies have shown that the in vivo anti tumor activity of an anti-CD137-mAb is dependent on Fc-gamma-R driven mechanisms and does not rely on agonistic activity only.

[0012] There is a need in the art for novel CD137 receptor agonists that exhibit high biological activity independent of Fc-gamma-R based crosslinking in vivo, high stability, and allow for efficient recombinant manufacturing.

SUMMARY OF THE INVENTION

[0013] The present invention provides specific CD137 receptor agonist proteins that mimic the CD137:CD137L interaction in vivo, exhibit low proteolytic degradation and a shorter in vivo half-life as compared to agonistic monoclonal antibodies.

[0014] The CD137 receptor agonist proteins of the instant invention generally comprise: (i) a first soluble CD137L cytokine domain; (ii) a first peptide linker; (iii) a second soluble CD137L domain; (iv) a second peptide linker; (v) a third soluble CD137L domain; (vi) a third peptide linker (e.g., a hinge-linker) and (vii) an antibody Fc fragment.

[0015] In one embodiment, the antibody Fc fragment (vii) is located N terminal to the first CD137L domain (i) and/or C-terminal to the third CD137L domain (v). In another embodiment the antibody Fc fragment is located C-terminally to the third CD137L domain (v). In one embodiment, the polypeptide is substantially non-aggregating. In another embodiment, the second and/or third soluble CD137L domain is an N-terminally shortened domain which optionally comprises amino acid sequence mutations. In another embodiment, the soluble CD137L domains (i), (ii) and (iii) are an C-terminally shortened domain which optionally comprises amino acid sequence mutations.

[0016] In one embodiment, at least one of the soluble CD137L domains, particularly at least one of the soluble CD137L domains (iii) and (v), is a soluble CD137L domain with an N-terminal sequence which starts at amino acid D86 or R88 or Q89 or G90 of human CD137L and wherein D86 or R88 or Q89 may be replaced by a neutral amino acid, e.g., Ser or Gly. In another embodiment, at least one of the soluble CD137L domains, particularly at least one of the soluble CD137L domains (iii) and (v), is a soluble CD137L domain with an N-terminal sequences selected from (a) D86-G90 and (b) (Gly/Ser)89-G90. In one embodiment, the soluble CD137L domain ends with amino acid E254 of human CD137L and/or optionally comprises one or more mutation at positions D86, L87, R88, Q89, D112, V118, A154, A174, A176, A188, T241. In one embodiment, the soluble CD137L domains (i), (iii) and (v) comprise amino acids D86-E254 of human CD137L according to SEQ ID NO: 1.

[0017] In one embodiment, at least one of the soluble CD137L domains, particularly at least the soluble CD137L domains (i), is a soluble CD137L domain with an N-terminal sequence which starts at amino acid R88 and wherein R88 may be replaced by Ser or Gly. In one embodiment, at least one of the soluble CD137L domains, particularly at least the soluble CD137L domain (iii), is a soluble C-terminal shortened CD137L domain ending with V240. In another embodiment, at least one of the soluble CD137L domains, particularly at least the soluble CD137L domains (iii), is a soluble C-terminal shortened CD137L domain ending with T241. In still another embodiment, at least one of the soluble CD137L domains, particularly at least the soluble CD137L domains (iii), is a soluble C-terminal shortened CD137L domain ending with E243.

[0018] In one embodiment, the first and second peptide linkers (ii) and (iv) independently have a length of 3-8 amino acids, particularly a length of 3, 4, 5, 6, 7, or 8 amino acids, and preferably are glycine/serine linkers, optionally comprising an asparagine residue which may be glycosylated. In one embodiment, the first and the second peptide linkers (ii) and (iv) consist of the amino acid sequence according to SEQ ID NO: 2. In another embodiment, the polypeptide additionally comprises an N-terminal signal peptide domain, e.g., of SEQ ID NO: 17, which may comprise a protease cleavage site, and/or which additionally comprises a C-terminal element which may comprise and/or connect to a recognition/purification domain, e.g., a Strep-tag attached to a serine linker according to SEQ ID NO: 18.

[0019] In one embodiment, the antibody Fc fragment (vii) is fused to the soluble CD137L domain (i) and/or (v) via a hinge-linker, preferably of SEQ ID NO: 16. In another embodiment, the antibody Fc fragment (vii) consists of the amino acid sequence as shown in SEQ ID NO: 13 or 14.

[0020] In one embodiment, the single-chain fusion polypeptide of the present invention comprises the amino acid sequence selected from the group consisting of SEQ ID NO: 15, and 25-35.

[0021] In one embodiment, the present invention provides a CD137 receptor agonist protein comprising a dimer of two single-chain fusion polypeptides each having the amino acid sequence set forth in SEQ ID NO: 27. In one embodiment, the two polypeptides are covalently linked through three interchain disulfide bonds formed between cysteine residues 484, 490, and 493 of each polypeptide. Similar cysteine residues are positions 484, 490 and 493 of SEQ ID NO: 28, 29 or 32, positions 489, 495 and 498 of SEQ ID NO: 30, positions 493, 499 and 502 of SEQ ID NO: 31, and positions 487, 493 and 496 of SEQ ID NO: 33 or 34.

[0022] In one embodiment, one or more of the asparagine residues at positions 158 and 318 of the mature polypeptide(s) SEQ ID NO: 27, 28 or 29 are N-glycosylated. In another embodiment, the asparagine residues at positions 158 and 318 of the polypeptide(s) are both N-glycosylated. Similar asparagine residues are positions 161 and 324 of SEQ ID NO: 30 or 31, and positions 159 and 320 of SEQ ID NO: 33 or 34.

[0023] In another embodiment, the polypeptide(s) are further post-translationally modified. In another embodiment, the post-translational modification comprises the N-terminal glutamine of the D86Q mutein of the first soluble domain (i) modified to pyroglutamate.

[0024] In still another embodiment, the post-translational modification comprises the N-terminal glutamine of the first soluble domain (i) starting with Q89 modified to pyroglutamate.

DESCRIPTION OF THE FIGURES

[0025] FIG. 1 Domain structure of a single-chain fusion polypeptide comprising three CD137L domains. I., II., III. Soluble CD137L domains.

[0026] FIG. 2 Schematic picture representing the general structure of CD137L. .box-solid. .box-solid. .box-solid. Cell membrane, N-terminus located within the cell,

[0027] 1. anti-parallel .beta.-fold of receptor-binding domain (RBD),

[0028] 2. interface of RBD and cell membrane,

[0029] 3. protease cleavage site.

[0030] FIG. 3 Single-chain fusion polypeptide comprising an additional Fab antibody fragment.

[0031] FIG. 4 Dimerization of two C-terminally fused single-chain Fc fusion polypeptides via three disulfide bridges.

[0032] FIG. 5 Schematic representation of the hexavalent single chain CD27 receptor agonist fusion protein of the invention. CH2-Carbohydrates (5) present on the inner surface areas normally shield the CH2-subdomain sterically (2) from proteases during "open Fc-conformation transits" wherein hinge-interchain disulfide bonds (4) are reduced and the covalent interchain linkage is disrupted. This enables CH2-dissociation and exposure of the inner surface areas and the upper hinge lysine K223 (6) towards proteases. Dimer association in the "open stage" remains intact due to the high affinity of the CH3 domains (3) to each other.

[0033] (1) scCD27L-RBD; (2) CH2 domain; (3) CH3 domain; (4) Hinge-Cysteines (left side: oxidized to disulfide bridges; right side reduced stage with free thiols); (5) CH2-Carbohydrates attached to N297 position (EU-numbering); (6) Upper Hinge Lysine (K223)

[0034] FIG. 6 ELISA assessing the binding of CD137 receptor agonist protein (Protein A) to its receptor

[0035] FIG. 7 Analytical size exclusion chromatography of strep tagged Protein A (SEQ ID NO: 28) performed on a 1260 Infinity HPLC system using a Tosoh TSKgelG3000SWxl column. The column was loaded with protein at a concentration of 1 mg/ml in a total volume of 20 .mu.l. The flow rate was set to 0.5 ml/min. One observes a single main peak at 16.97 min for Protein A. The low molecular weight buffer components of the sample elute after one column volume (>23.5 min).

[0036] FIG. 8 SDS-PAGE results of Protein A under non-reducing and reducing conditions. 360 ng of Protein A were loaded on an SDS-PAGE 4-12% Bis-Tris gel under non-reducing (Lane 1) or reducing (Lane 2) conditions containing DTT as reducing agent. Gels were run at 130V for 15 min followed by 180V for 60 min and were subsequently stained using a silver-stain protocol. One observes a molecular weight difference between the main bands in A and B of about 70-80 kDa. As this is about half the molecular weight as observed for the main band in lane 1, this indicates that the homodimer in lane 2 is covalently linked by disulfide bridges. The bonds are lost under reducing conditions in lane 2

DETAILED DESCRIPTION OF THE INVENTION

[0037] The present invention provides a single-chain fusion polypeptide comprising at least three soluble CD137L domains connected by two peptide linkers and N-terminally and/or C-terminally an antibody-derived dimerization domain. The inventors have discovered that dimerization of the two single-chain fusion polypeptides through the dimerization domain results in a hexavalent CD137 receptor agonist, which provides high biological activity and good stability.

[0038] Preferably, the single-chain fusion polypeptide is non-aggregating. The term "non-aggregating" refers to a monomer content of the preparation of .gtoreq.50%, preferably .gtoreq.70% and more preferably .gtoreq.90%. The ratio of monomer content to aggregate content may be determined by examining the amount of aggregate formation using size-exclusion chromatography (SEC). The stability concerning aggregation may be determined by SEC after defined time periods, e.g. from a few to several days, to weeks and months under different storage conditions, e.g. at 4.degree. C. or 25.degree. C. For the fusion protein, in order to be classified as substantially non-aggregating, it is preferred that the "monomer" content is as defined above after a time period of several days, e.g. 10 days, more preferably after several weeks, e.g. 2, 3 or 4 weeks, and most preferably after several months, e.g. 2 or 3 months of storage at 4.degree. C., or 25.degree. C. With regard to the definition of "monomer" in the case of FC-fusion proteins, the assembly of two polypeptide chains is driven by the FC-part and the functional unit of the resulting assembled protein consists of two chains. This unit is defined as "monomer" in the case of Fc-fusion proteins regardless of being a dimerized single-chain fusion polypeptide.

[0039] The single-chain fusion polypeptide may comprise additional domains which may be located at the N- and/or C-termini thereof. Examples for additional fusion domains are e.g. an N-terminal signal peptide domain which may comprise a protease cleave site or a C-terminal element which may comprise and/or connect to a recognition/purification domain. According to a preferred embodiment, the fusion polypeptide comprises a Strep-tag at its C-terminus that is fused via a linker. An exemplary Strep-tag including a short serine linker is shown in SEQ ID NO: 18.

[0040] The CD137 receptor agonist protein of the present invention comprises three soluble domains derived from CD137L. Preferably, those soluble domains are derived from a mammalian, particularly human CD137L including allelic variants and/or derivatives thereof. The soluble domains comprise the extracellular portion of CD137L including the receptor binding domain without membrane located domains. Like other proteins of the TNF superfamily, CD137L is anchored to the membrane via an N-terminal portion of 15-30 amino acids, the so-called stalk-region. The stalk region contributes to trimerization and provides a certain distance to the cell membrane. However, the stalk region is not part of the trimeric receptor binding domain (RBD) with the receptor binding sites located at the protomer interfaces.

[0041] Importantly, the RBD of the Tumor Necrosis Factor Superfamily is characterized by a particular localization of its N- and C-terminal amino acids. Said amino acids are immediately adjacent and are located in close proximity to the axis of the trimer. The first N-terminal amino acids of the RBD form an anti-parallel beta-strand with a C-terminal region of the RBD. Thus, the aforementioned anti-parallel beta-strand of the RBD forms an interface with the cell membrane, which is connected to and anchored within the cell membrane via the amino acids of the stalk region.

[0042] Human CD137L contains a stalk region as well as most likely a C-terminal extension (V240-E254).

[0043] It is highly preferred that the soluble CD137L domains of the CD137 receptor agonist protein comprise a receptor binding domain of the CD137L lacking any amino acids from the stalk region. Otherwise, a long linker connecting the C-terminus of one of the soluble domains with the N-terminus of the next soluble domain would be required to compensate for the N-terminal stalk-region of the next soluble domain, which might result in instability and/or formation of aggregates. For the same reason, it is also highly preferred that the soluble CD137L domains of the CD137 receptor agonist protein comprise a receptor binding domain of the CD137L lacking any amino acids from the C-terminal extension.

[0044] A further advantage of such soluble domains is that the N-terminal amino acids of the RBD are not accessible for any anti-drug antibodies. Preferably, the single-chain fusion polypeptide consisting of (i) a first soluble CD137L domain; (ii) a first peptide linker; (iii) a second soluble CD137L domain; (iv) a second peptide linker; (v) a third soluble CD137L domain is capable of forming an ordered structure mimicking the trimeric organization of its natural counterpart thereby comprising at least one functional binding site for the respective CD137L receptor. The single-chain fusion polypeptide comprising components (i)-(v) is therefore also termed single-chain-CD137L-receptor-binding-domain (scCD137L-RBD). Importantly, compared to homotrimeric wild type CD137L-RBD, the scCD137L-RBD comprises an enhanced stability as the soluble CD137L domains (i), (iii) and (v) are enforced to trimerize by the covalent linkage to each other provided by the linkers (ii) and (iv).

[0045] The CD137 receptor agonist protein comprises three functional CD137 receptor binding sites, i.e. amino acid sequences capable of forming a complex with a CD137 receptor. Thus, the soluble domains are capable of binding to the corresponding CD137 receptor. In one embodiment, at least one of the soluble domains is capable of receptor activation, whereby apoptotic and/or proliferative activity may be affected. In a further embodiment, one or more of the soluble domains are selected as not being capable of receptor activation.

[0046] The soluble CD137L domain may be derived from human CD137L as shown in SEQ ID NO: 1. Preferably, the soluble CD137L domains are derived from human CD137L, particularly starting from amino acids 86, 88, 89 or 90 and comprise particularly amino acids 86-254 or 88-254 or 89-254 of SEQ ID NO: 1. Optionally, amino acid R88 of SEQ ID NO: 1 may be replaced by a noncharged amino acid, e.g. Ser or Gly or is replaced by Glutamine.

TABLE-US-00001 TABLE 1 Sequence of Wild-Type Human CD137L Protein SEQ ID NO Sequence 1 MEYASDASLDPEAPWPPAPRARACRVLPWALVAGLLLLLLLA AACAVFLACPWAVSGARASPGSAASPRLREGPELSPDDPAGL LDLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSY KEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQ PLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQ RLGVHLHTEARARHAWQLTQGATVLGLFRVTPEIPAGLPSPR SE

[0047] As indicated above, the soluble CD137L domains may comprise the wild-type sequences as indicated in SEQ ID NO: 1. It should be noted, however, that it is possible to introduce mutations in one or more of these soluble domains, e.g. mutations which alter (e.g. increase or decrease) the binding properties of the soluble domains. In one embodiment, soluble domains that cannot bind to the corresponding cytokine receptor can be selected.

[0048] In a further embodiment of the invention, the soluble CD137L domain (i) comprises a mutant of CD137L or a receptor binding domain thereof resulting in reduced affinity and/or reduced activation of CD137 receptor.

[0049] CD137L-Muteins Affecting Receptor Binding and/or Activity

[0050] The mutant may be generated by any technique known by a skilled person. The substitution may affect at least one amino acid of CD137L, e.g., human CD137L (e.g., SEQ ID NO: 1) or a receptor binding domain thereof as described herein. Preferred substitutions in this regard affect at least one of the following amino acids of human CD137L of SEQ ID NO: 1: L115, K127, R150, R193 and Q227.

[0051] In another preferred embodiment, the C-terminal region I243-E254 is deleted from at least one of the soluble domains (i), (III) or (v).

[0052] The amino acid substitution(s) may affect the binding and/or activity of CD137L, e.g., human CD137L, to or on either the CD137 binding or the CD137 induced signaling. The binding and/or activity of the CD137 may be affected positively, i.e., stronger, more selective or more specific binding and/or more activation of the receptor. Alternatively, the binding and/or activity of the CD137 may be affected negatively, i.e., weaker, less selective or less specific binding and/or less or no activation of the receptor.

[0053] Thus one embodiment is a CD137 receptor agonist protein as described herein wherein at least one of the soluble domains comprises a mutant of CD137L or a receptor binding domain thereof which binds and/or activates CD137 to a lesser extent than the wildtype-CD137L.

[0054] CD137L-Muteins with Enhanced Stability/Solubility

[0055] In a further embodiment of the invention, one or more of the soluble CD137L domains (i), (iii), and (v) may comprise a mutant of CD137L or a receptor binding domain thereof resulting in reduced self-aggregation and/or prolonged in vivo stability. A174, A176. Preferred substitutions in this regard are A174[D, N] and A176[S, T]. The mutation(s) of each CD137L domain may be the same or different.

[0056] The single-chain fusion molecule of the present invention comprises three soluble CD137L domains, namely components (i), (iii) and (v). The stability of a single-chain CD137L fusion polypeptide against aggregation is enhanced, if the second and/or third soluble CD137L domain is an N-terminally shortened domain which optionally comprises amino acid sequence mutations. Thus, preferably, both the second and the third soluble CD137L domain are N-terminally shortened domains which optionally comprise amino acid sequence mutations in the N-terminal regions, preferably within the first five amino acids of the N-terminus of the soluble CD137L domain. These mutations may comprise replacement of basic amino acids, by neutral amino acids, particularly serine or glycine.

[0057] In contrast thereto, the selection of the first soluble CD137L domain is not as critical. Here, a soluble domain having a full-length N-terminal sequence may be used. It should be noted, however, that also the first soluble CD137L domain may have an N-terminally shortened and optionally mutated sequence.

[0058] In a further preferred embodiment of the present invention, the soluble CD137L domains (i), (iii) and (v) are soluble human CD137L domains. The first soluble CD137L domain (i) may be selected from native, shortened and/or mutated sequences. Thus, the first soluble CD137L domain (i) has a N-Terminal sequence which may start at amino acid D86 or R88 of human CD137L, and wherein R88 may be replaced by a neutral amino acid, e.g. by Ser or Gly or by Gln to enable pyroglutamate formation during expression. The second and third soluble CD137L domains (iii) and (v) have a shortened N-terminal sequence which preferably starts with amino acid Q89 or G90 of human CD137L (SEQ ID NO:1) and wherein Q89 may be replaced by another amino acid, e.g. Ser or Gly.

[0059] Preferably, the N-terminal sequence of the soluble CD137L domains (iii) and (v) is selected from:

[0060] (a) D86 or Q89

[0061] (b) (Gly/Ser) 89

[0062] The soluble CD137L domain preferably ends with amino acid E254 of human CD137L. In certain embodiments, the CD137L domain may comprise internal mutations as described above.

[0063] In another preferred embodiment, the soluble CD137L domain preferably ends with amino acid V240 of human CD137L. In certain embodiments, the CD137L domain may comprise internal mutations as described above.

[0064] Components (ii) and (iv) of the CD137 receptor agonist protein are peptide linker elements located between components (i) and (iii) or (iii) and (v), respectively. The flexible linker elements have a length of 3-8 amino acids, particularly a length of 3, 4, 5, 6, 7, or 8 amino acids. The linker elements are preferably glycine/serine linkers, i.e. peptide linkers substantially consisting of the amino acids glycine and serine. In cases in in which the soluble cytokine domain starts with S or G (N-terminus), the linker ends before this S or G.

[0065] It should be noted that linker (ii) and linker (iv) do not need to be of the same length. In order to decrease potential immunogenicity, it may be preferred to use shorter linkers. In addition it turned out that shorter linkers lead to single chain molecules with reduced tendency to form aggregates. Whereas linkers that are substantially longer than the ones disclosed here may exhibit unfavorable aggregations properties.

[0066] If desired, the linker may comprise an asparagine residue which may form a glycosylate site Asn-Xaa-Ser. In certain embodiments, one of the linkers, e.g. linker (ii) or linker (iv) comprises a glycosylation site. In other embodiments, both linkers (iv) comprise glycosylation sites. In order to increase the solubility of the CD137L agonist proteins and/or in order to reduce the potential immunogenicity, it may be preferred that linker (ii) or linker (iv) or both comprise a glycosylation site.

[0067] Preferred linker sequences are shown in Table 2. A preferred linker is GSGSGNGS (SEQ ID NO: 2).

TABLE-US-00002 TABLE 2 Example Linker Sequences SEQ ID NO Sequence 2 GSGSGNGS 3 GSGSGSGS 4 GGSGSGSG 5 GGSGSG 6 GGSG 7 GGSGNGSG 8 GGNGSGSG 9 GGNGSG 10 GSGSGS 11 GSGS 12 GSG

[0068] The CD137 receptor agonist protein additionally comprises an antibody Fc fragment domain which may be located N-terminal to the first CD137L domain (i) and/or C-terminal to the third CD137L domain (v). Preferably, the antibody Fc fragment domain comprises a reduced capability to interact with Fc-gamma-R receptors in vivo. Preferably, the antibody Fc fragment domain comprises or consists of an amino acid sequence as shown in SEQ ID NO: 13 or 14 (see Table 3). Sequence ID NO: 13 has N297S mutation compared to wildtype human IGG1-Fc. Sequence ID NO: 14 is a glycosylated (N297 wildtype) human IGG1 Fc mutein with reduced Fc-gamma-R binding capability.

TABLE-US-00003 TABLE 3 Examples of Fc Fragment Domains SEQ ID NO Sequence 13 PAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDP EVKFNWYVDGVEVHNAKTKPREEQYSSTYRVVSVLTVLHQDW LNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR EEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPV LDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQK SLSLSPGK 14 PAPPVAGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPE VKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWL NGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSRE EMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK

[0069] Number of Glycosylation Sites and In Vivo Stability

[0070] The total number of glycosylation sites and the individual position of the carbohydrates in three dimensions impacts the in-vivo stability of CD137 receptor agonist proteins. Further, carbohydrate recognition depends on local density of the terminal saccharides, the branching of the carbohydrate tree and the relative position of the carbohydrates to each other matter.

[0071] Further, partially degraded carbohydrates reduce the in vivo half-life of CD137 receptor agonist proteins through lectin-driven mechanisms. By reducing the total number of glycosylation sites on the molecule, the resulting compound is less accessible to these mechanisms, increasing half-life.

[0072] Depletion of the CH2-domain carbohydrates of the Fc-domain is necessary in order to avoid Fc-gamma-Receptor based binding. FcR-gamma-Receptors on cells could lead to hyper-crosslinking of the fusion-protein in vivo potential leading to CD137-receptor superclustering-based toxicity. Also, unwanted Fc-driven mechanisms like ADCC could lead to toxic events. Accordingly, in one embodiment, the overall number of glycosylation sites on the CD137 receptor agonist proteins of the instant invention is reduced through the depletion of CH2 glycosylation sites, particularly the N-glycosylation site, resulting in CD137 receptor agonist proteins comprising N297S equivalent mutations of SEQ ID NO: 15 (PROTEIN A) (according to the EU numbering system) creating aglycosl-CH2 domains.

[0073] CH2-Domain Destabilization is Compensated by an Additional Hinge-Cysteine

[0074] CH2-glycosylation present on the inner surface areas normally shields the subdomain from proteases during "open Fc-conformation transits" wherein hinge-interchain disulfide bonds are reduced and the covalent interchain linkage is disrupted. This enables CH2-dissociation and exposure of the inner surface area towards proteases. CD137 receptor agonist proteins comprising an Fc-domain with a N297S equivalent mutation of SEQ ID NO: 15 (PROTEIN A) (according to the EU numbering system) creates an a glycosylated-CH2 and are therefore likely to be subject to protease digestion and less stable than equivalent structures with wild-type CH2 glycosylation. This would impact the compound's stability during USP/DSP/storage, where host cell proteases are present and have long-term access to the structure. Accordingly, in certain embodiments, the CD137 receptor agonist lacks CH2 glycosylation sites, but comprises glycosylation sites in the linker sequences of each polypeptide chain (e.g., GSGSGNGS, SEQ ID NO: 2).

[0075] According to a preferred embodiment of the invention, the antibody Fc fragment domain is fused via a hinge linker element. The hinge-linker element has a length of 10-30 amino acids, particularly a length of 15-25 amino acids, e.g. 22 amino acids. The term "hinge-linker" includes any linker long enough to allow the domains attached by the hinge linker element to attain a biologically active confirmation. The hinge-linker element preferably comprises the hinge-region sequence of an immunoglobulin, herein referred to as "Ig hinge-region". The term "Ig hinge region" means any polypeptide comprising an amino acid sequence that shares sequence identity or similarity with a portion of a naturally occurring Ig hinge-region sequence which includes one or more cysteine residues, e.g., two cysteine residues, at which the disulfide bonds link the two heavy chains of the immunoglobulin.

[0076] Derivatives and analogues of the hinge-region can be obtained by mutations. A derivative or analogue as referred to herein is a polypeptide comprising an amino acid sequence that shares sequence identity or similarity with the full length sequence of the wild type (or naturally occurring protein) except that it has one or more amino acid sequence differences attributable to a deletion, insertion and/or substitution.

[0077] The number of molecules with open Fc-conformation in an individual CD137 receptor agonist protein depends on the number of interchain-disulfide bonds present in the hinge region. Accordingly, in one embodiment a third cysteine (C225 according to the EU numbering system) was introduced into the hinge region of the CD137 receptor agonist proteins of the instant invention in order to ameliorate the effect of depleting the CH2-glycosites.

[0078] Exchange of a Lysine to Glycine in the Hinge Region Results in Enhanced Proteolytic Stability

[0079] In one embodiment, the CD137 receptor agonist proteins of the invention additionally comprise a mutation of the upper-hinge lysine (K223, according to the EU numbering system) to a glycine to reduce proteolytic processing at this site, thereby enhancing the overall stability of the fusion protein. Combining aforementioned introduction of a third cysteine (C225, according to the EU numbering system) with the aforementioned lysine to glycine mutation (K223G, according to the EU numbering system) within the hinge region results in an overall stabilized CD137 receptor agonist protein of the instant invention.

[0080] A particularly preferred hinge-linker element including the aforementioned cysteine (C225) and the lysine to glycine mutation (K223G) comprises or consists of the amino acid sequence as shown in SEQ ID NO: 16 (Table 4).

[0081] The CD137 receptor agonist protein may additionally comprise an N-terminal signal peptide domain, which allows processing, e.g. extracellular secretion, in a suitable host cell. Preferably, the N-terminal signal peptide domain comprises a protease cleavage site, e.g. a signal peptidase cleavage site and thus may be removed after or during expression to obtain the mature protein. A particularly preferred N-terminal signal peptide domain comprises the amino acid sequence as shown in SEQ ID NO: 17 (Table 4).

[0082] Further, the CD137 receptor agonist protein may additionally comprise a C-terminal element, having a length of e.g. 1-50, preferably 10-30 amino acids which may include or connect to a recognition/purification domain, e.g. a FLAG domain, a Streptag or Strep-tag II domain and/or a poly-His domain. According to a preferred embodiment, the fusion polypeptide comprises a Streptag fused to the C-terminus via a short serine linker as shown in SEQ ID NO: 18 (Table 4).

[0083] Preferred hinge-linker elements (SEQ ID NO: 16, 19-24), a preferred N-terminal signal peptide domain (SEQ ID NO: 17) and a preferred serine linker-strep tag (SEQ ID NO: 18) are shown in Table 4.

TABLE-US-00004 TABLE 4 Exemplary domains and linkers SEQ ID NO Sequence 16 GSSSSSSSSGSCDKTHTCPPC 17 METDTLLVFVLLVWVPAGNG 18 SSSSSSAWSHPQFEK 19 GSSSSSSSGSCDKTHTCPPC 20 GSSSSSSGSCDKTHTCPPC 21 GSSSSSGSCDKTHTCPPC 22 GSSSGSCDKTHTCPPC 23 GSSSGSCDKTHTCPPCGS 24 GSSSGSCDKTHTCPPCGSGS

[0084] In one embodiment of the invention, the fusion polypeptide comprises three soluble CD137L domains fused by peptide linker elements of SEQ ID NO: 2. All three soluble CD137L domain (i), (iii), (v) consists of amino acids 89-240 of human CD137L according to SEQ ID NO: 1. The resulting scCD137L-RBD sequence module is shown in table 5b SEQ ID NO: 36.

[0085] In a further preferred embodiment of the invention, the fusion polypeptide comprises three soluble CD137L domains fused by peptide linker elements of SEQ ID NO: 2. All three soluble CD137L domain (i), (iii), (v) consists of amino acids 86-240 of human CD137L according to SEQ ID NO: 1 with D86Q mutation in the first domain (i). The resulting scCD137L-RBD sequence module is shown in table 5b SEQ ID NO: 39.

[0086] In another embodiment of the invention, the fusion polypeptide comprises three soluble CD137L domains fused by peptide linker elements of SEQ ID NO: 2. All three soluble CD137L domain (i), (iii), (v) consists of amino acids 88-240 of human CD137L according to SEQ ID NO: 1. The resulting scCD137L-RBD sequence module is shown in table 5b SEQ ID NO: 40.

[0087] In still another preferred embodiment of the invention, the fusion polypeptide comprises three soluble CD137L domains fused by peptide linker elements of SEQ ID NO: 2. All three soluble CD137L domain (i), (iii), (v) consists of amino acids 88-240 of human CD137L according to SEQ ID NO: 1 with R88Q mutation in the first domain (i) and R88G mutation in domains (iii) and (v). The resulting scCD137L-RBD sequence module is shown in table 5b SEQ ID NO: 41.

[0088] In still another preferred embodiment of the invention, the fusion polypeptide comprises three soluble CD137L domains fused by peptide linker elements of SEQ ID NO: 2. All three soluble CD137L domain (i), (iii), (v) consists of amino acids 88-240 of human CD137L according to SEQ ID NO: 1 with R88S mutation in the first domain (i) and R88G mutation in domains (iii) and (v). The resulting scCD137L-RBD sequence module is shown in table 5b SEQ ID NO: 42.

[0089] In still another preferred embodiment of the invention, the fusion polypeptide comprises three soluble CD137L domains fused by peptide linker elements of SEQ ID NO: 2. All three soluble CD137L domain (i), (iii), (v) consists of amino acids 89-240 of human CD137L according to SEQ ID NO: 1 and comprise the A174N and A176S mutations. The resulting scCD137L-RBD sequence module is shown in table 5b SEQ ID NO: 43.

[0090] The aforementioned scCD137L-RBD modules (SEQ ID: 36, 39-43) are well suited to generate fusion proteins with additional domains fused to either N- or C-terminal end employing the linkers described in Table 2 (SEQ ID NO: 2-12).

[0091] Preferred Configuration CD137L-Fc

[0092] Additionally, the fusion polypeptide comprises an antibody Fc fragment domain according to SEQ ID NO: 13 that is fused C-terminally to the soluble CD137L domain (v) via a hingelinker according to SEQ ID NO: 16. The inventors surprisingly found that this particular fusion polypeptide provides improved biological activity as compared to bivalent agonistic anti-CD137-mAB and has a prolonged stability as compared to fusion proteins comprising a lysine in position 223 and a N297S mutation in the CH2 domain (according to the EU numbering).

[0093] The amino acid sequence of an exemplary embodiment of a CD137 receptor agonist protein of the invention is set forth in SEQ ID NO: 27.

[0094] Further, the fusion polypeptide may comprise an N-terminal signal peptide domain e.g. according to SEQ ID NO: 17. A specific example of a CD137 receptor agonist protein of the invention is shown in SEQ ID NO: 25.

[0095] According to another preferred embodiment, the fusion polypeptide may additionally comprise a C-terminal Strep-tag that is fused to the polypeptide of the invention via a short serine linker as shown in SEQ ID NO: 18. According to this aspect of the invention, the Fc fragment preferably consists of the amino acid sequence as shown in SEQ ID NO: 13 or 14. Further, the Fc fragment may consist of a shorter Fc fragment, for example including amino acids 1217 of SEQ ID NO: 13. Particularly preferred examples of fusion polypeptides comprising a C-terminal Strep-tag are shown in SEQ ID NO: 15 (PROTEIN A).

[0096] The exemplary CD137 receptor agonist proteins as shown in SEQ ID Nos: 15, 25, and 26, each comprises an N-terminal signal peptide domain, at amino acids 1-20 of each sequence. In each case, the mature protein starts with amino acid 21. Mature exemplary CD137 receptor agonist proteins (without a signal peptide) of the instant invention are set forth in SEQ ID NO: 27-35. Exemplary CD137 receptor agonist proteins described above are shown in Table 5.

[0097] The CD137 receptor agonist as set forth in SEQ ID NO: 27 has a reduced total number of glycosylation sites (the N297S mutation in the CH2 region providing an a glycosylated CH2 domain, according to the EU numbering system), an increased number of interchain disulfide bonds in the hinge region, and the mutation of an upper-hinge lysine to a glycine (K223G, according to the EU numbering system). These alterations provide a decrease in potential degradation and CD137 receptor superclustering (along with concomitant toxicity).

[0098] The CD137 receptor agonist as set forth in SEQ ID NO: 30 comprises a scCD137L-RBD module with SEQ ID NO: 36, a third peptide linker with SEQ ID NO: 21 and (vii) an antibody Fc fragment with SEQ ID NO: 13.

[0099] The CD137 receptor agonist as set forth in SEQ ID NO: 31 comprises a scCD137L-RBD module with SEQ ID NO: 39, a third peptide linker with SEQ ID NO: 16 and (vii) an antibody Fc fragment with SEQ ID NO: 13.

[0100] The CD137 receptor agonist as set forth in SEQ ID NO: 32 comprises a scCD137L-RBD module with SEQ ID NO: 40, a third peptide linker with SEQ ID NO: 16 and (vii) an antibody Fc fragment with SEQ ID NO: 13.

[0101] The CD137 receptor agonist as set forth in SEQ ID NO: 33 comprises a scCD137L-RBD module with SEQ ID NO: 41, a third peptide linker with SEQ ID NO: 16 and (vii) an antibody Fc fragment with SEQ ID NO: 13.

[0102] The CD137 receptor agonist as set forth in SEQ ID NO: 34 comprises a scCD137L-RBD module with SEQ ID NO: 42, a third peptide linker with SEQ ID NO: 16 and (vii) an antibody Fc fragment with SEQ ID NO: 13.

TABLE-US-00005 TABLE 5 Exemplary CD137 receptor agonist proteins SEQ ID NO Sequence 25 METDTLLVFVLLVWVPAGNGQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLS PROTEIN A YKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALT without VDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFR StrepTag VGSGSGNGSQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVA KAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEAR NSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQG MFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQL ELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLL HLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPC PAPELLGGPSVFLFPPKPKDTLMISRTPEVICVVVDVSHEDPEVKFNWYVDGVEVHN AKTKPREEQYSSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVYTLPPSREEMTKNQVSLICLVKGFYPSDIAVEWESNGQPENNYKTIPPVLDS DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 15 METDTLLVFVLLVWVPAGNGQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLS PROTEIN A YKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALT VDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFR VGSGSGNGSQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVA KAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEAR NSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQG MFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQL ELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLL HLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPC PAPELLGGPSVFLFPPKPKDTLMISRTPEVICVVVDVSHEDPEVKFNWYVDGVEVHN AKTKPREEQYSSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQP REPQVYTLPPSREEMTKNQVSLICLVKGFYPSDIAVEWESNGQPENNYKTIPPVLDS DGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGSSSSSSAWSHP QFEK 26 METDTLLVFVLLVWVPAGNGQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLS CD137L-wt + YKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALT SEQ14 VDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFR VGSGSGNGSQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVA KAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEAR NSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQG MFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQL ELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLL HLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGS5555SSSGSCDKTHTCPPC PAPPVAGPSVFLFPPKPKDTLMISRIPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNA KTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPR EPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 27 QGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFF CD137L-wt + QLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGR SEQ13 FC LLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQN No Signal VLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGE No Strep GSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLG No Glyco VHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQNVLLIDGPLSWY SDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHL QPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARH AWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPCPAPELLGGPSVFLFPPKPKD TLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLSPGK 28 QGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFF Deg lyco-Fc QLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGR No Signal + LLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQN StrepTag VLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGE GSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLG VHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQNVLLIDGPLSWY SDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHL QPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARH AWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPCPAPELLGGPSVFLFPPKPKD TLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLSPGSSSSSSAWSHPQFEK 29 QGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFF Glyco FC QLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGR No Signal LLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQN No strep VLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGE GSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLG VHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQNVLLIDGPLSWY SDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHL QPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARH AWQLTQGATVLGLFRVGS5555SSSGSCDKTHTCPPCPAPPVAGPSVFLFPPKPKDT LMISRIPEVICVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLT VLHQDWLNGKEYKCKVSNKGLPSSIEKTISKAKGQPREPQVYTLPPSREEMTKNQVS LTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGN VFSCSVMHEALHNHYTQKSLSLSPGK 30 QLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYY SEQ39 + FC VFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGF 13 QGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSDLRQGMFA Linker 21 QLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELR RVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLS AGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSDLRQGMFAQLVAQNVL LIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGS GSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVH LHTEARARHAWQLTQGATVLGLFRVGSSSSGSCDKTHTCPPCPAPELLGGPSVFLFP PKPKDILMISRIPEVICVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYR VVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEM TKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKS RWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 31 QLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYY VFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGF QGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSDLRQGMFA QLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELR RVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLS AGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSDLRQGMFAQLVAQNVL LIDGPLSWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGS GSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVH LHTEARARHAWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPCPAPELLGGPSV FLFPPKPKDTLMISRIPEVICVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYS STYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPS REEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT VDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK 32 RQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVF FQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQG RLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSRQGMFAQLVA QNVLLIDGPLSWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVA GEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQR LGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSRQGMFAQLVAQNVLLIDGPL SWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLA LHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEAR ARHAWQLTQGATVLGLFRVGSSSSSGSCDKTHTCPPCPAPELLGGPSVFLFPPKPKD ILMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLSPGK 33 QQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVF FQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQG RLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSgQGMFAQLVA QNVLLIDGPLSWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVA GEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQR LGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSgQGMFAQLVAQNVLLIDGPL SWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLA LHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEAR ARHAWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPCPAPELLGGPSVFLFPPK PKDILMISRIPEVICVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYRVV SVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTK NQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRW QQGNVFSCSVMHEALHNHYTQKSLSLSPGK 34 SQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVF FQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQG RLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSgQGMFAQLVA QNVLLIDGPLSWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVA GEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQR LGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSgQGMFAQLVAQNVLLIDGPL SWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLA LHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEAR ARHAWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPCPAPELLGGPSVFLFPPK PKDTLMISRTPEVICVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYRVV SVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTK NQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRW QQGNVFSCSVMHEALHNHYTQKSLSLSPGK 35 QGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFF (Seq27 with QLELRRVVAGEGSGSVSLALHLQPLRSANGSAALALTVDLPPASSEARNSAFGFQGR additional LLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQN glycol-sites) VLLIDGPLSWYSDPGLAGVSLIGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGE GSGSVSLALHLQPLRSANGSAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLG VHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQNVLLIDGPLSWY SDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHL QPLRSANGSAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARH AWQLTQGATVLGLFRVGSSSSSSSSGSCDKTHTCPPCPAPELLGGPSVFLFPPKPKD TLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYSSTYRVVSVL TVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQG NVFSCSVMHEALHNHYTQKSLSLSPGK

TABLE-US-00006 TABLE 5B Exemplary scCD137L-RBD modules 36 QGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVA KAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVD LPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGA TVLGLFRVGSGSGNGSQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSL TGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQ PLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHL HTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQNVLLID GPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRV VAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQ GRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRV 39 QLRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKEL VVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALAL TVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLT QGATVLGLFRVGSGSGNGSDLRQGMFAQLVAQNVLLIDGPLSWYSDPG LAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVS LALHLQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQ RLGVHLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSDLRQGMFAQL VAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYV FFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSE ARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFR V 40 RQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVV AKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTV DLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQG ATVLGLFRVGSGSGNGSRQGMFAQLVAQNVLLIDGPLSWYSDPGLAGV SLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALH LQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGV HLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSRQGMFAQLVAQNVL LIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLEL RRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAF GFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRV 41 QQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVV AKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTV DLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQG ATVLGLFRVGSGSGNGSgQGMFAQLVAQNVLLIDGPLSWYSDPGLAGV SLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALH LQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGV HLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSgQGMFAQLVAQNVL LIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLEL RRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAF GFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRV 42 SQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVV AKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALTV DLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQG ATVLGLFRVGSGSGNGSgQGMFAQLVAQNVLLIDGPLSWYSDPGLAGV SLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALH LQPLRSAAGAAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGV HLHTEARARHAWQLTQGATVLGLFRVGSGSGNGSgQGMFAQLVAQNVL LIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLEL RRVVAGEGSGSVSLALHLQPLRSAAGAAALALTVDLPPASSEARNSAF GFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRV 43 QGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSLTGGLSYKEDTKELVVA KAGVYYVFFQLELRRVVAGEGSGSVSLALHLQPLRSANGSAALALTVD LPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEARARHAWQLTQGA TVLGLFRVGSGSGNGSQGMFAQLVAQNVLLIDGPLSWYSDPGLAGVSL TGGLSYKEDTKELVVAKAGVYYVFFQLELRRVVAGEGSGSVSLALHLQ PLRSANGSAALALTVDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHL HTEARARHAWQLTQGATVLGLFRVGSGSGNGSQGMFAQLVAQNVLLID GPLSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVFFQLELRRV VAGEGSGSVSLALHLQPLRSANGSAALALTVDLPPASSEARNSAFGFQ GRLLHLSAGQRLGVHLHTEARARHAWQLTQGATVLGLFRV

[0103] A further aspect of the present invention relates to a nucleic acid molecule encoding a CD137 receptor agonist protein as described herein. The nucleic acid molecule may be a DNA molecule, e.g. a double-stranded or single-stranded DNA molecule, or an RNA molecule. The nucleic acid molecule may encode the CD137 receptor agonist protein or a precursor thereof, e.g. a pro- or pre-proform of the CD137 receptor agonist protein which may comprise a signal sequence or other heterologous amino acid portions for secretion or purification which are preferably located at the N- and/or C-terminus of the CD137 receptor agonist protein. The heterologous amino acid portions may be linked to the first and/or second domain via a protease cleavage site, e.g. a Factor X3, thrombin or IgA protease cleavage site. A specific example of a nucleic acid sequence of the invention is shown in Table 6 as SEQ ID NO: 37. This nucleic acid molecule comprises the open reading frame encoding the fusion polypeptide of SEQ ID NO: 25.

TABLE-US-00007 TABLE 6 Nucleic Acid Sequence of Exemplary C0137 receptor agonist protein SEQ ID NO Sequence 37 AAGCTTTAGGGATAACAGGGTAATAGCCGCCACCATGGAGAC TGACACCCTGCTGGTGTTCGTGCTGCTGGTCTGGGTGCCTGC AGGAAATGGACAGGGCATGTTCGCTCAACTGGTCGCACAGAA CGTGCTGCTCATTGACGGTCCCCTGTCTTGGTACTCCGATCC AGGGTTGGCAGGAGTGTCCTTGACAGGAGGGCTGTCCTATAA GGAGGATACCAAAGAGCTGGTGGTAGCAAAGGCTGGTGTGTA TTACGTGTTCTTTCAGCTGGAGCTGCGCAGAGTCGTCGCAGG CGAAGGATCTGGTAGTGTGTCACTGGCACTGCACTTGCAGCC CCTTCGGTCCGCTGCCGGGGCAGCAGCACTGGCCCTGACCGT CGATCTGCCACCCGCTTCTAGCGAGGCACGAAACTCAGCCTT TGGGTTTCAGGGTCGCCTGCTGCACCTGAGCGCCGGACAGAG GCTGGGCGTTCATCTGCACACCGAGGCCAGAGCCAGACACGC TTGGCAGTTGACTCAGGGAGCTACGGTCCTCGGTCTGTTTCG AGTAGGCAGCGGAAGCGGCAATGGCTCTCAGGGCATGTTTGC TCAGCTGGTAGCCCAGAACGTACTCCTGATCGATGGCCCTCT TTCATGGTACTCAGACCCCGGACTGGCCGGAGTTAGCCTTAC AGGTGGGCTTAGTTATAAGGAGGACACAAAGGAATTGGTTGT GGCCAAAGCTGGCGTGTACTATGTGTTCTTCCAGCTTGAGCT CCGCAGAGTCGTGGCTGGGGAGGGCTCTGGCAGTGTGAGCCT TGCCCTTCATCTGCAACCTTTGCGGAGCGCAGCCGGCGCTGC TGCACTGGCCCTTACAGTGGATTTGCCACCCGCAAGTAGTGA AGCTCGCAATTCCGCATTCGGTTTCCAGGGCCGTCTGCTCCA TCTTTCTGCCGGTCAACGTCTGGGAGTTCACCTCCACACTGA GGCTAGGGCCAGGCATGCTTGGCAGCTGACTCAAGGAGCCAC TGTCTTGGGACTCTTTCGGGTAGGCTCCGGGTCTGGCAACGG CTCCCAGGGGATGTTTGCCCAACTGGTCGCCCAGAATGTCCT GCTCATCGATGGTCCTCTGAGCTGGTATTCCGACCCTGGACT GGCTGGTGTGAGCCTGACTGGCGGACTCTCCTACAAAGAGGA CACCAAGGAACTGGTGGTGGCCAAAGCCGGGGTGTACTACGT GTTCTTCCAGTTGGAACTGCGGCGGGTTGTGGCTGGCGAGGG ATCAGGTTCCGTTAGTCTGGCCCTGCACCTCCAGCCTCTGAG GTCTGCTGCTGGTGCCGCCGCTCTGGCCTTGACCGTCGACCT CCCACCCGCATCTTCCGAAGCCCGAAATTCAGCCTTCGGGTT CCAGGGCAGACTGCTGCATCTGAGTGCTGGACAGCGCCTTGG GGTTCATCTCCACACCGAAGCCAGGGCCCGACATGCCTGGCA GCTCACACAAGGCGCAACCGTTTTGGGGCTCTTTCGTGTGgg atcctcgagTTCATCGTCCTCATCCGGCTCATGTGATAAGAC CCACACCTGCCCTCCCTGTCCTGCCCCTGAGCTGCTGGGCGG ACCTTCTGTGTTCCTGTTCCCCCCCAAGCCTAAGGACACCCT GATGATCTCCAGGACCCCTGAGGTGACCTGTGTGGTGGTGGA CGTGTCTCACGAAGATCCCGAGGTGAAGTTCAACTGGTACGT GGACGGCGTGGAGGTCCACAACGCCAAGACCAAGCCTAGGGA GGAGCAGTACAGCTCCACCTACCGGGTGGTGTCTGTGCTGAC CGTGCTGCACCAGGATTGGCTGAACGGAAAGGAGTATAAGTG TAAGGTCTCCAACAAGGCCCTGCCTGCCCCCATCGAGAAAAC CATCTCCAAGGCCAAGGGCCAGCCTCGGGAGCCTCAGGTGTA CACCCTGCCTCCTAGCAGGGAGGAGATGACCAAGAACCAGGT GTCCCTGACCTGTCTGGTGAAGGGCTTCTACCCTTCCGATAT CGCCGTGGAGTGGGAGTCTAATGGCCAGCCCGAGAACAACTA CAAGACCACCCCTCCTGTGCTGGACTCTGACGGCTCCTTCTT CCTGTACTCCAAGCTGACCGTGGACAAGTCCAGATGGCAGCA GGGCAACGTGTTCTCCTGCTCCGTGATGCACGAGGCCCTGCA CAATCACTACACCCAGAAGTCCCTGTCTCTGAGTCCGGGCAA GTAATAggcgcgcc

[0104] The nucleic acid molecule may be operatively linked to an expression control sequence, e.g. an expression control sequence which allows expression of the nucleic acid molecule in a desired host cell. The nucleic acid molecule may be located on a vector, e.g. a plasmid, a bacteriophage, a viral vector, a chromosomal integration vector, etc. Examples of suitable expression control sequences and vectors are described for example by Sambrook et al. (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, and Ausubel et al. (1989), Current Protocols in Molecular Biology, John Wiley & Sons or more recent editions thereof.

[0105] Various expression vector/host cell systems may be used to express the nucleic acid sequences encoding the CD137 receptor agonist proteins of the present invention. Suitable host cells include, but are not limited to, prokaryotic cells such as bacteria, e.g. E. coli, eukaryotic host cells such as yeast cells, insect cells, plant cells or animal cells, preferably mammalian cells and, more preferably, human cells. Further, the invention relates to a non-human organism transformed or transfected with a nucleic acid molecule as described above. Such transgenic organisms may be generated by known methods of genetic transfer including homologous recombination.

[0106] A further aspect of the present invention relates to a pharmaceutical or diagnostic composition comprising as the active agent at least one CD137 receptor agonist protein, a respective nucleic acid encoding therefore, or a transformed or transfected cell, all as described herein.

[0107] In another aspect, the present invention provides a pharmaceutical composition comprising an CD137 receptor agonist protein disclosed herein and one or more pharmaceutically acceptable carriers, diluents, excipients, and/or adjuvants. In another aspect, the present invention provides a nucleic acid molecule encoding the CD137 receptor agonist protein. In another embodiment, the present invention provides an expression vector comprising the nucleic acid molecule. In another embodiment, the present invention provides a cell comprising the nucleic acid molecule. In a further embodiment, the cell is a eukaryotic cell. In another embodiment, the cell is a mammalian cell. In another embodiment, the cell is a Chinese Hamster Ovary (CHO) cell. In other embodiments, the cell is selected from the group consisting of CHO-DBX11, CHO-DG44, CHO-S, and CHO-K1 cells. In other embodiments, the cell is selected from the group consisting of Vero, BHK, HeLa, COS, MDCK, HEK-293, NIH-3T3, W138, BT483, Hs578T, HTB2, BT20, T47D, NS0, CRL7030, HsS78Bst, PER.C6, SP2/0-Agl4, and hybridoma cells.

[0108] In another aspect, the present invention provides a method of treating a subject having an CD137L-associated disease or disorder, the method comprising administering to the subject an effective amount of the CD137 receptor agonist protein. In one embodiment, the CD137 receptor agonist protein is administered alone. In another embodiment, the CD137 receptor agonist protein is administered before, concurrently, or after the administration of a second agent. In another embodiment, the disease or disorder is selected from the group consisting of: tumors, infectious diseases, inflammatory diseases, metabolic diseases, autoimmune disorders, degenerative diseases, apoptosis-associated diseases, and transplant rejections. In one embodiment, the tumors are solid tumors. In one embodiment, the tumors arise from the group of cancers consisting of sarcoma, esophageal cancer, and gastric cancer. In another embodiment, the tumors arise from Ewing's sarcoma or fibrosarcoma. In another embodiment, the tumors arise from the group of cancers consisting of Non-Small Cell Lung Carcinoma (NSCLC), pancreatic cancer, colorectal cancer, breast cancer, ovarian cancer, head and neck cancers, and Small Cell Lung Cancer (SCLC). In another embodiment, the tumors are lymphatic tumors. In one embodiment, the tumors are hematologic tumors. In another embodiment, the tumors arise from non-Hodgkin's lymphoma, leukemia, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), B cell lymphoma, Burkitt's lymphoma, chronic myelocytic leukemia (CML), chronic lymphocytic leukemia (CLL), or hairy cell leukemia. In another embodiment, the autoimmune disorders are rheumatoid diseases, arthritic diseases, or rheumatoid and arthritic diseases. In a further embodiment, the disease or disorder is rheumatoid arthritis. In another embodiment, the degenerative disease is a neurodegenerative disease. In a further embodiment, the neurodegenerative disease is multiple sclerosis.

[0109] In one embodiment, the second agent is a chemotherapeutic, radiotherapeutic, or biological agent. In one embodiment, the second agent is selected from the group consisting of Duvelisib, Ibrutinib, Navitoclax, and Venetoclax. In another embodiment, the second agent is an apoptotic agent. In one embodiment, the apoptotic second agent is selected from the group consisting of Bortezomib, Azacitidine, Dasatinib, and Gefitinib. In a particular embodiment, the pharmaceutical compositions disclosed herein are administered to a patient by intravenous or subcutaneous administration. In other embodiments, the disclosed pharmaceutical compositions are administered to a patient byoral, parenteral, intramuscular, intrarticular, intrabronchial, intraabdominal, intracapsular, intracartilaginous, intracavitary, intracelial, intracerebellar, intracerebroventricular, intracolic, intracervical, intragastric, intrahepatic, intramyocardial, intraosteal, intrapelvic, intrapericardiac, intraperitoneal, intrapleural, intraprostatic, intrapulmonary, intrarectal, intrarenal, intraretinal, intraspinal, intrasynovial, intrathoracic, intrauterine, intravesical, bolus, vaginal, rectal, buccal, sublingual, intranasal, or transdermal administration.

[0110] In one embodiment, the CD137 receptor agonist protein is administered as a single bolus. In another embodiment, CD137 receptor agonist protein may be administered over several divided doses. The CD137 receptor agonist protein can be administered at about 0.1-100 mg/kg. In one embodiment, the CD137 receptor agonist protein can be administered at a dosage selected from the group consisting of: about 0.1-0.5, 0.1-1, 0.1-10, 0.1-20, 0.1-50, 0.1-75, 1-10, 1-15, 1-7.5, 1.25-15, 1.25-7.5, 2.5-7.5, 2.5-15, 5-15, 5-7.5, 1-20, 1-50, 7-75, 1-100, 5-10, 5-15, 5-20, 5-25, 5-50, 5-75, 10-20, 10-50, 10-75, and 10-100 mg/kg. In other embodiments, the CD137 receptor agonist protein is present in pharmaceutical compositions at about 0.1-100 mg/ml. In one embodiment, the CD137 receptor agonist protein is present in pharmaceutical compositions at an amount selected from the group consisting of: about 0.1-0.5, 0.1-1, 0.1-10, 0.1-20, 0.1-50, 0.1-75, 1-10, 1-20, 1-50, 1-75, 1-100, 5-10, 5-15, 5-20, 5-25, 5-50, 5-75, 10-20, 10-50, 10-75, or 10-100 mg/ml. In other embodiments, a therapeutically effective amount of CD137 receptor agonist protein is administered to a subject. In another embodiment, a prophylactically effective amount of CD137 receptor agonist protein is administered to a subject.

[0111] The term "CD137L-associated disease or disorder" as used herein is any disease or disorder which may be ameliorated by administering an effective amount of a CD137 receptor agonist to a subject in need thereof. At least one CD137 receptor agonist protein, respective nucleic acid encoding therefore, or transformed or transfected cell, all as described herein may be used in therapy, e.g., in the prophylaxis and/or treatment of disorders caused by, associated with and/or accompanied by dysfunction of CD137L, particularly proliferative disorders, such as tumors, e.g. solid or lymphatic tumors; infectious diseases; inflammatory diseases; metabolic diseases; autoimmune disorders, e.g. rheumatoid and/or arthritic diseases; degenerative diseases, e.g. neurodegenerative diseases such as multiple sclerosis; apoptosis-associated diseases or transplant rejections.

[0112] The term "dysfunction of CD137L" as used herein is to be understood as any function or expression of CD137L that deviates from the normal function or expression of CD137L, e.g., overexpression of the CD137L gene or protein, reduced or abolished expression of the CD137L gene or protein compared to the normal physiological expression level of CD137L, increased activity of CD137L, reduced or abolished activity of CD137L, increased binding of CD137L to any binding partners, e.g., to a receptor, particularly a CD137L receptor or another cytokine molecule, reduced or abolished binding to any binding partner, e.g. to a receptor, particularly a CD137L receptor or another cytokine molecule, compared to the normal physiological activity or binding of CD137L.

[0113] In various embodiments, a method is provided for diagnosing and/or treating a human subject suffering from a disorder which can be diagnosed and/or treated by targeting CD137L receptors comprising administering to the human subject a CD137 receptor agonist protein disclosed herein such that the effect on the activity of the target, or targets, in the human subject is agonistic, one or more symptoms is alleviated, and/or treatment is achieved. The CD137 receptor agonist proteins provided herein can be used to diagnose and/or treat humans suffering from primary and metastatic cancers, including carcinomas of breast, colon, rectum, lung (e.g., small cell lung cancer "SCLC" and non-small cell lung cancer "NSCLC"), oropharynx, hypopharynx, esophagus, stomach, pancreas, liver, gallbladder and bile ducts, small intestine, urinary tract (including kidney, bladder and urothelium), female genital tract (including cervix, uterus, and ovaries as well as choriocarcinoma and gestational trophoblastic disease), male genital tract (including prostate, seminal vesicles, testes and germ cell tumors), endocrine glands (including the thyroid, adrenal, and pituitary glands), and skin, as well as hemangiomas, melanomas, sarcomas (including those arising from bone and soft tissues as well as Kaposi's sarcoma), tumors of the brain, nerves, eyes, and meninges (including astrocytomas, gliomas, glioblastomas, retinoblastomas, neuromas, neuroblastomas, Schwannomas, and meningiomas), tumors arising from hematopoietic malignancies, acute leukemia, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), B cell lymphoma, Burkitt's lymphoma, chronic myelocytic leukemia (CML), chronic lymphocytic leukemia (CLL), hairy cell leukemia, Hodgkin's and non-Hodgkin's lymphomas, DLBCL, follicular lymphomas, hematopoietic malignancies, Kaposi's sarcoma, malignant lymphoma, malignant histiocytosis, malignant melanoma, multiple myeloma, paraneoplastic syndrome/hypercalcemia of malignancy, or solid tumors.

[0114] A pharmaceutical composition comprising a CD137 receptor agonist protein disclosed herein and a pharmaceutically acceptable carrier is provided. In some embodiments, the pharmaceutical composition comprises at least one additional therapeutic agent for treating a disorder. For example, the additional agent may be a therapeutic agent, a chemotherapeutic agent; an imaging agent, a cytotoxic agent, an angiogenesis inhibitor, a kinase inhibitor (including but not limited to a KDR and a TIE-2 inhibitor), a co-stimulation molecule modulator or an immune checkpoint inhibitor (including but not limited to anti-B7.1, anti-B7.2, anti-B7.3, anti-B7.4, anti-CD28, anti-B7RP1, CTLA4-Ig, anti-CTLA-4, anti-PD-1, anti-PD-L1, anti-PD-L2, anti-ICOS, anti-LAG-3, anti-Tim3, anti-VISTA, anti-HVEM, anti-BTLA, LIGHT fusion protein, anti-CD137, anti-CD137L, anti-OX40, anti-OX40L, anti-CD70, anti-CD27, anti-CD27L, anti-GAL9, anti-A2AR, anti-KIR, anti-IDO-1, anti-CD20), a dendritic cell/antigen-presenting cell modulator (including but not limited to anti-CD40 antibody, anti-CD40L, anti-DC-SIGN, anti-Dectin-1, anti-CD301, anti-CD303, anti-CD123, anti-CD207, anti-DNGR1, anti-CD205, anti-DCIR, anti-CD206, anti-ILT7), a modulator for Toll-like receptors (including but not limited to anti-TLR-1, anti-TLR-2, anti-TLR-3, anti-TLR-4, anti-TLR-4, anti-TLR-5, anti-TLR-6, anti-TLR-7, anti-TLR-8, anti-TLR-9), an adhesion molecule blocker (including but not limited to an anti-LFA-1 antibody, an anti-E/L selectin antibody, a small molecule inhibitor), an anti-cytokine antibody or functional fragment thereof (including but not limited to an anti-IL-18, an anti-TNF, or an anti-IL-6/cytokine receptor antibody), a bispecific redirected T cell or NK cell cytotoxicity (including but not limited to a BiTE.RTM.), a chimeric T cell receptor (CAR-T) based therapy, a T cell receptor (TCR)-based therapy, a therapeutic cancer vaccine, methotrexate, cyclosporin, rapamycin, FK506, a detectable label or reporter, a TNF antagonist, an anti-rheumatic, a muscle relaxant, a narcotic, a non-steroid anti-inflammatory drug (NSAID), an analgesic, an anesthetic, a sedative, a local anesthetic, a neuromuscular blocker, an antimicrobial, an antipsoriatic, a corticosteriod, an anabolic steroid, an erythropoietin, an immunization, an immunoglobulin, an immunosuppressive, a growth hormone, a hormone replacement drug, a radiopharmaceutical, an antidepressant, an antipsychotic, a stimulant, an asthma medication, a beta agonist, an inhaled steroid, an epinephrine or analog, a cytokine, or a cytokine antagonist.

[0115] In an embodiment, a method of treating a cancer or in the prevention or inhibition of metastases from the tumors described herein, the CD137 receptor agonist protein(s) can be used alone or in combination with one or more additional agents, e.g., a chemotherapeutic, radiotherapy, or biological agent. In some embodiments, the agent can include the following: 13-cis-Retinoic Acid; 2-CdA; 2-Chlorodeoxyadenosine; 5-Azacitidine; 5-Fluorouracil; 5-FU; 6-Mercaptopurine; 6-MP; 6-TG; 6-Thioguanine; Abraxane; Accutane.RTM.; Actinomycin-D; Adriamycin.RTM.; Adrucil.RTM.; Afinitor.RTM.; Agrylin.RTM.; Ala-Cort.RTM.; Aldesleukin; Alemtuzumab; ALIMTA; Alitretinoin; Alkaban-AQ.RTM.; Alkeran.RTM.; All-transretinoic Acid; Alpha Interferon; Altretamine; Amethopterin; Amifostine; Aminoglutethimide; Anagrelide; Anandron.RTM.; Anastrozole; Arabinosylcytosine; Ara-C Aranesp.RTM.; Aredia.RTM.; Arimidex.RTM.; Aromasin.RTM.; Arranon.RTM.; Arsenic Trioxide; Arzerra.TM. Asparaginase; ATRA; Avastin.RTM.; Azacitidine; BCG; BCNU; Bendamustine; Bevacizumab; Bexarotene; BEXXAR.RTM.; Bicalutamide; BiCNU; Blenoxane.RTM.; Bleomycin; Bortezomib; Busulfan; Busulfex.RTM.; C225; Calcium Leucovorin; Campath.RTM.; Camptosar.RTM.; Camptothecin-11; Capecitabine Carac.TM.; Carboplatin; Carmustine; Carmustine Wafer; Casodex.RTM.; CC-5013; CCI-779; CCNU; CDDP; CeeNU; Cerubidine.RTM.; Cetuximab; Chlorambucil; Cisplatin; Citrovorum Factor; Cladribine; Cortisone; Cosmegen.RTM.; CPT-11; Cyclophosphamide; Cytadren.RTM.; Cytarabine; Cytarabine Liposomal; Cytosar-U.RTM.; Cytoxan.RTM.; Dacarbazine; Dacogen; Dactinomycin; Darbepoetin Alfa; Dasatinib; Daunomycin; Daunorubicin; Daunorubicin Hydrochloride; Daunorubicin Liposomal; DaunoXome.RTM.; Decadron; Decitabine; Delta-Cortef.RTM.; Deltasone.RTM.; Denileukin; Diftitox; DepoCyt.TM.; Dexamethasone; Dexamethasone Acetate; Dexamethasone Sodium Phosphate; Dexasone; Dexrazoxane; DHAD; DIC; Diodex; Docetaxel; Doxil.RTM.; Doxorubicin; Doxorubicin Liposomal; Droxia.TM.; DTIC; DTIC-Dome.RTM.; Duralone.RTM.; Duvelisib; Efudex.RTM.; Eligard.TM.; Ellence.TM.; Eloxatin.TM. Elspar.RTM.; Emcyt.RTM.; Epirubicin; Epoetin Alfa; Erbitux; Erlotinib; Erwinia L-asparaginase; Estramustine; Ethyol Etopophos.RTM.; Etoposide; Etoposide Phosphate; Eulexin.RTM.; Everolimus; Evista.RTM.; Exemestane; Fareston.RTM.; Faslodex.RTM.; Femara.RTM.; Filgrastim; Floxuridine; Fludara.RTM.; Fludarabine; Fluoroplex.RTM.; Fluorouracil; Fluorouracil (cream); Fluoxymesterone; Flutamide; Folinic Acid; FUDR.RTM.; Fulvestrant; Gefitinib; Gemcitabine; Gemtuzumab ozogamicin; Gemzar; Gleevec.TM.; Gliadel.RTM. Wafer; GM-CSF; Goserelin; Granulocyte-Colony Stimulating Factor (G-CSF); Granulocyte Macrophage Colony Stimulating Factor (G-MCSF); Halotestin.RTM.; Herceptin.RTM.; Hexadrol; Hexalen.RTM.; Hexamethylmelamine; HMM; Hycamtin.RTM.; Hydrea.RTM.; Hydrocort Acetate.RTM.; Hydrocortisone; Hydrocortisone Sodium Phosphate; Hydrocortisone Sodium Succinate; Hydrocortone Phosphate; Hydroxyurea; Ibrutinib; Ibritumomab; Ibritumomab Tiuxetan; Idamycin.RTM.; Idarubicin Ifex.RTM.; Interferon-alpha; Interferon-alpha-2b (PEG Conjugate); Ifosfamide; Interleukin-11 (IL-11); Interleukin-2 (IL-2); Imatinib mesylate; Imidazole Carboxamide; Intron A.RTM.; ipilimumab, Iressa.RTM.; Irinotecan; Isotretinoin; Ixabepilone; Ixempra.TM.; KADCYCLA.RTM.; Kidrolase (t) Lanacort.RTM.; Lapatinib; L-asparaginase; LCR; Lenalidomide; Letrozole; Leucovorin; Leukeran; Leukine.TM.; Leuprolide; Leurocristine; Leustatin.TM. Lirilumab; Liposomal Ara-C; Liquid Pred.RTM.; Lomustine; L-PAM; L-Sarcolysin; Lupron.RTM.; Lupron Depot.RTM.; Matulane.RTM.; Maxidex; Mechlorethamine; Mechlorethamine Hydrochloride; Medralone.RTM.; Medrol.RTM.; Megace.RTM.; Megestrol; Megestrol Acetate; MEK inhibitors; Melphalan; Mercaptopurine; Mesna; Mesnex.TM.; Methotrexate; Methotrexate Sodium; Methylprednisolone; Meticorten.RTM.; Mitomycin; Mitomycin-C; Mitoxantrone M-Prednisol.RTM.; MTC; MTX; Mustargen.RTM.; Mustine; Mutamycin.RTM.; Myleran.RTM.; Mylocel.TM.; Mylotarg.RTM.; Navitoclax; Navelbine.RTM.; Nelarabine; Neosar.RTM.; Neulasta.TM.; Neumega.RTM.; Neupogen.RTM.; Nexavar.RTM.; Nilandron.RTM.; Nilotinib; Nilutamide; Nipent.RTM.; Nitrogen Mustard Novaldex.RTM.; Nivolumab; Novantrone.RTM.; Nplate; Octreotide; Octreotide acetate; Ofatumumab; Oncospar.RTM.; Oncovin.RTM.; Ontak.RTM.; Onxal.TM.; Oprelvekin; Orapred.RTM.; Orasone.RTM.; Oxaliplatin; Paclitaxel; Paclitaxel Protein-bound; Pamidronate; Panitumumab; Panretin.RTM.; Paraplatin.RTM.; Pazopanib; Pediapred.RTM.; PEG Interferon; Pegaspargase; Pegfilgrastim; PEG-INTRON.TM.; PEG-L-asparaginase; PEMETREXED; Pembrolizumab; Pentostatin; Pertuzumab; Phenylalanine Mustard; Pidilizumab; Platinol.RTM.; Platinol-AQ.RTM.; Prednisolone; Prednisone; Prelone.RTM.; Procarbazine; PROCRIT.RTM.; Proleukin.RTM.; Prolifeprospan 20 with Carmustine Implant; Purinethol.RTM.; BRAF inhibitors; Raloxifene; Revlimid.RTM.; Rheumatrex.RTM.; Rituxan.RTM.; Rituximab; Roferon-A.RTM.; Romiplostim; Rubex.RTM.; Rubidomycin hydrochloride; Sandostatin.RTM.; Sandostatin LAR.RTM.; Sargramostim; Solu-Cortef.RTM.; Solu-Medrol.RTM.; Sorafenib; SPRYCEL.TM.; STI-571; STIVAGRA.TM., Streptozocin; SU11248; Sunitinib; Sutent.RTM.; Tamoxifen Tarceva.RTM.; Targretin.RTM.; Tasigna.RTM.; Taxol.RTM.; Taxotere.RTM.; Temodar.RTM.; Temozolomide Temsirolimus; Teniposide; TESPA; Thalidomide; Thalomid.RTM.; TheraCys.RTM.; Thioguanine; Thioguanine Tabloid.RTM.; Thiophosphoamide; Thioplex.RTM.; Thiotepa; TICE.RTM.; Toposar.RTM.; Topotecan; Toremifene; Torisel.RTM.; Tositumomab; Trastuzumab; Treanda.RTM.; Tremelimumab; Tretinoin; Trexall.TM.; Trisenox.RTM.; TSPA; TYKERB.RTM.; Urelumab; VCR; Vectibix.TM. Velban.RTM.; Velcade.RTM.; Venetoclax; VePesid.RTM.; Vesanoid.RTM.; Viadur.TM.; Vidaza.RTM.; Vinblastine; Vinblastine Sulfate; Vincasar Pfs.RTM.; Vincristine; Vinorelbine; Vinorelbine tartrate; VLB; VM-26; Vorinostat; Votrient; VP-16; Vumon.RTM.; Xeloda.RTM.; Zanosar.RTM.; Zevalin.TM. Zinecard.RTM.; Zoladex.RTM.; Zoledronic acid; Zolinza; or Zometa.RTM., and/or any other agent not specifically listed here that target similar pathways.

[0116] When two or more substances or principles are to be used as part of a combined treatment regimen, they can be administered via the same route of administration or via different routes of administration, at essentially the same time or at different times (e.g. essentially simultaneously, consecutively, or according to an alternating regime). When the substances or principles are to be administered simultaneously via the same route of administration, they may be administered as different pharmaceutical formulations or compositions or part of a combined pharmaceutical formulation or composition, as will be clear to the skilled person.

[0117] Also, when two or more active substances or principles are to be used as part of a combined treatment regimen, each of the substances or principles may be administered in the same amount and according to the same regimen as used when the compound or principle is used on its own, and such combined use may or may not lead to a synergistic effect. However, when the combined use of the two or more active substances or principles leads to a synergistic effect, it may also be possible to reduce the amount of one, more than one, or all of the substances or principles to be administered, while still achieving the desired therapeutic action. This may, e.g., be useful for avoiding, limiting or reducing any unwanted side-effects that are associated with the use of one or more of the substances or principles when they are used in their usual amounts, while still obtaining the desired pharmaceutical or therapeutic effect.

[0118] The effectiveness of the treatment regimen used according to the invention may be determined and/or followed in any manner known per se for the disease or disorder involved, as will be clear to the clinician. The clinician will also be able, where appropriate and on a case-by-case basis, to change or modify a particular treatment regimen, so as to achieve the desired therapeutic effect, to avoid, limit or reduce unwanted side-effects, and/or to achieve an appropriate balance between achieving the desired therapeutic effect on the one hand and avoiding, limiting or reducing undesired side effects on the other hand.

[0119] Generally, the treatment regimen will be followed until the desired therapeutic effect is achieved and/or for as long as the desired therapeutic effect is to be maintained. Again, this can be determined by the clinician.

[0120] In various embodiments, pharmaceutical compositions comprising one or more CD137 receptor agonist proteins, either alone or in combination with prophylactic agents, therapeutic agents, and/or pharmaceutically acceptable carriers are provided herein. In various embodiments, nonlimiting examples of the uses of the pharmaceutical compositions disclosed herein include diagnosing, detecting, and/or monitoring a disorder, preventing, treating, managing, and/or ameliorating a disorder or one or more symptoms thereof, and/or in research. The formulation of pharmaceutical compositions, either alone or in combination with prophylactic agents, therapeutic agents, and/or pharmaceutically acceptable carriers, are known to one skilled in the art (US Patent Publication No. 20090311253 A1).

[0121] As used herein, the phrase "effective amount" means an amount of CD137L agonist protein that results in a detectable improvement (e.g., at least about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, or more from baseline) in one or more parameters associated with a dysfunction of CD137L or with a CD137L-associated disease or disorder.

[0122] Methods of administering a therapeutic agent provided herein include, but are not limited to, oral administration, parenteral administration (e.g., intradermal, intramuscular, intraperitoneal, intravenous and subcutaneous), epidural administration, intratumoral administration, mucosal administration (e.g., intranasal and oral routes) and pulmonary administration (e.g., aerosolized compounds administered with an inhaler or nebulizer). The formulation of pharmaceutical compositions for specific routes of administration, and the materials and techniques necessary for the various methods of administration are available and known to one skilled in the art (US Patent Publication No. 20090311253 A1).

[0123] In various embodiments, dosage regimens may be adjusted to provide for an optimum desired response (e.g., a therapeutic or prophylactic response). For example, a single bolus may be administered, several divided doses may be administered over time or the dose may be proportionally reduced or increased as indicated by the exigencies of the therapeutic situation. In some embodiments, parenteral compositions are formulated in dosage unit form for ease of administration and uniformity of dosage. The term "dosage unit form" refers to physically discrete units suited as unitary dosages for the mammalian subjects to be treated; each unit containing a predetermined quantity of active compound calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier.

[0124] An exemplary, non-limiting range for a therapeutically or prophylactically effective amount of a CD137 receptor agonist protein provided herein is about 0.1-100 mg/kg, (e.g., about 0.1-0.5, 0.1-1, 0.1-10, 0.1-20, 0.1-50, 0.1-75, 1-10, 1-15, 1-7.5, 1.25-15, 1.25-7.5, 2.5-7.5, 2.5-15, 5-15, 5-7.5, 1-20, 1-50, 7-75, 1-100, 5-10, 5-15, 5-20, 5-25, 5-50, 5-75, 10-20, 10-50, 10-75, or 10-100 mg/kg, or any concentration in between). In some embodiments, the CD137 receptor agonist protein is present in a pharmaceutical composition at a therapeutically effective concentration, e.g., a concentration of about 0.1-100 mg/ml (e.g., about 0.1-0.5, 0.1-1, 0.1-10, 0.1-20, 0.1-50, 0.1-75, 1-10, 1-20, 1-50, 1-75, 1-100, 5-10, 5-15, 5-20, 5-25, 5-50, 5-75, 10-20, 10-50, 10-75, or 10-100 mg/ml, or any concentration in between). Note that dosage values may vary with the type and/or severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens may be adjusted over time according to the individual need and/or the professional judgment of the person administering or supervising the administration of the compositions, and that dosage ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed composition.

EXAMPLES

[0125] 1.1 Polypeptide Structure

[0126] A) Amino acids Met1-Gly20

[0127] Ig-Kappa-signal peptide, assumed signal peptidase cleavage site after amino acid Gly 20.

[0128] B) Amino acids Gln21-Val172

[0129] First soluble cytokine domain of the human CD137L ligand (CD137L, amino acid 89-240 of SEQ ID NO: 1).

[0130] C) Amino acids Gly173-Ser 180

[0131] First peptide linker element of SEQ ID NO: 2.

[0132] D) Amino acids Gln181-Val332

[0133] Second soluble cytokine domain of the human CD137L ligand (CD137L, amino acid 89-240 of SEQ ID NO: 1).

[0134] E) Amino acids Gly333-Ser340.

[0135] Second peptide linker element of SEQ ID NO: 2.

[0136] F) Amino acids Gln341-Val492

[0137] Third soluble cytokine domain of the human CD137L ligand (CD137L, amino acid 89-240 of SEQ ID NO: 1).

[0138] G) Amino acids Gly493-Cys513

[0139] Hinge-linker element of SEQ ID NO: 16.

[0140] H) Amino acids Pro514-Lys731

[0141] Antibody Fc fragment domain of SEQ ID NO: 13.

[0142] The above CD137 receptor agonist protein is shown in SEQ ID NO: 25.

[0143] The indicated linkers may be replaced by other preferred linkers, e.g. as shown in SEQ ID NOs: 3-12.

[0144] The indicated Hinge-linker element may be replaced by other preferred Hinge-linkers, e.g. as shown in SEQ ID NOs: 19-24.

[0145] It should be noted that the first and second peptide linkers do not need to be identical.

[0146] The signal peptide sequence (A) may be replaced by any other suitable, e.g. mammalian signal peptide sequence.

[0147] 1.2 Gene Cassette Encoding the Polypeptide

[0148] The synthetic gene may be optimized in view of its codon usage for the expression in suitable host cells, e.g. insect cells or mammalian cells. A preferred nucleic acid sequence is shown in SEQ ID NO: 37.

Example 2. Expression and Purification

[0149] 2.1 Cloning, Expression and Purification of Fusion Polypeptides

[0150] The aforementioned fusion proteins are expressed recombinantly in different eukaryotic host cells employing the methods described below:

[0151] Method for Small Scale Expression of CD137 Receptor Agonist Fusion Proteins:

[0152] For small scale analysis of aforementioned CD137 receptor agonist fusion proteins, Hek293 cells are grown in DMEM+GlutaMAX (GibCo) supplemented with 10% FBS, 100 units/ml Penicillin and 100 [mu]g/ml Streptomycin and are transiently transfected with a plasmid containing an expression cassette for a fusion polypeptide and an appropriate selection marker, e.g. a functional expression cassette comprising a blasticidine, puromycin or hygromycin resistance gene. In those cases, where a plurality of polypeptide chains is necessary to achieve the final product, the expression cassettes are either combined on one plasmid or positioned on different plasm ids during the transfection. Cell culture supernatant containing recombinant fusion polypeptide are harvested three days post transfection and clarified by centrifugation at 300.times.g followed by filtration through a 0.22 .mu.m sterile filter.

[0153] Method for Large Scale Expression and Purification of CD137 Receptor Agonist Fusion Proteins

[0154] For larger scale expression of CD137 receptor agonist fusion proteins, synthetic DNA cassettes encoding the aforementioned proteins are inserted into eukaryotic expression vectors comprising appropriate selection markers (e.g. a functional expression cassette comprising a blasticidin, puromycin or hygromycin resistance gene) and genetic elements suitable to enhance the number of transcriptionally active insertion sites within the host cells genome. The sequence verified expression vectors is introduced by electroporation into suspension adapted Chinese Hamster Ovary cells (CHO-S, Invitrogen). Appropriate selection pressure will be applied three days post-transfection to transfected cells. Surviving cells carrying the vector derived resistance gene(s) are recovered by subsequent cultivation under selection pressure. Upon stable growth of the selected cell pools in chemically defined medium (PowerCHO2-CD, Lonza) at 37.degree. C. and 7% CO2 atmosphere in an orbital shaker incubator (100 rpm, 50 mm shaking throw), the individual supernatants are analyzed by ELISA-assays detecting the aforementioned proteins and the cell pools with the highest specific productivity are expanded in shake flasks prior to protein production (orbital shaker, 100 rpm, shaking throw 50 mm).

[0155] For lab-scale protein production, individual cell pools are cultured for 7-12 days in chemically defined medium (PowerCHO2-CD, Lonza) at 37.degree. C. and 7% CO2 atmosphere in a Wave bioreactor 20/50 EHT (GE-Healthcare). The basal medium is PowerCHO2-CD supplemented with 4 mM Glutamax. Wave culture is started with a viable cell concentration of 0.3 to 0.4.times.10e6 cells/ml and the following settings (for a five- or ten liter bag): shaking frequency 18 rpm, shaking ankle 7.degree., gas current 0.2-0.3 L/min, 7% CO2, 36.5.degree. C. During the Wave run, the cell culture is fed twice with PowerFeed A (Lonza), usually on day 2 (20% feed) and day 5 (30% feed). After the second feed, shaking frequency is increased to 22 rpm, as well as the shaking ankle to 8.degree..

[0156] The bioreactor is usually harvested in between day 7 to day 12 when the cell viability drops below 80%. First, the culture supernatant is clarified using a manual depth filtration system (Millipore Millistak Pod, MCOHC 0.054 m.sup.2). For Strep-tagged proteins, Avidin is added to a final concentration of 0.5 mg/L. Finally, the culture supernatant containing the CD137 receptor agonist fusion protein is sterile filtered using a bottle top filter (0.22 .mu.m, PES, Corning) and stored at 2-8.degree. C. until further processing.

[0157] For affinity purification Streptactin Sepharose is packed to a column (gel bed 2 ml), equilibrated with 15 ml buffer W (100 mM Tris-HCl, 150 mM NaCl, pH 8.0) or PBS pH 7.4 and the cell culture supernatant is applied to the column with a flow rate of approx. 4 ml/min. Subsequently, the column is washed with 15 ml buffer W and bound polypeptide is eluted stepwise by addition of 7.times.1 ml buffer E (100 mM Tris HCl, 150 mM NaCl, 2.5 mM Desthiobiotin, pH 8.0). Alternately, PBS pH 7.4 containing 2.5 mM Desthiobiotin can be used for this step.

[0158] Alternately to the Streptactin Sepharose based method, the affinity purification is performed employing a column with immobilized Protein-A as affinity ligand and an Akta chromatography system (GE-Healthcare). A solid phase material with high affinity for the FC-domain of the fusion protein is chosen: MABSelect Sure.TM. (GE Healthcare). Briefly, the clarified cell culture supernatant is loaded on a HiTrap MabSelectSure column (CV=5 ml) equilibrated in wash-buffer-1 (20 mM Pi, 95 mM NaCl, pH7.2) not exceeding a load of 10 mg fusion protein per ml column-bed. The column is washed with ten column-volumes (10 CV) of aforementioned equilibration buffer followed by four column-volumes (4 CV) of wash-buffer-2 (20 mM Pi, 95 mM NaCl, pH 8.0) to deplete host-cell protein and host-cell DNA. The column is then eluted with elution buffer (20 mM Pi, 95 mM NaCl, pH 3.5) and the eluate is collected in up to ten fractions with each fraction having a volume equal to column-bed volume (5 ml). Each fraction is neutralized with an equal volume of aforementioned wash-buffer-2. The linear velocity is set to 150 cm/h and kept constant during the aforementioned affinity chromatography method. The protein amount of the eluate fractions is quantitated and peak fractions are concentrated by ultrafiltration and further purified by size exclusion chromatography (SEC).

[0159] SEC is performed on Superdex 200 10/300 GL or HiLoad 26/60 columns using an Akta chromatography system (GE-Healthcare). The columns are equilibrated with phosphate buffered saline and the concentrated, affinity-purified polypeptide is loaded onto the SEC column with the sample volume not exceeding 2% (v/v) of the column-volume. In the case of Superdex 200 10/300 GL columns (GE Healthcare), a flow rate of 0.5 ml per minute is applied. In the case of HiLoad 26/60 Superdex200 columns, a flow rate of 2.5 ml per minute is applied. The elution profile of the polypeptide is monitored by absorbance at 280 nm.

[0160] For determination of the apparent molecular weight of purified fusion polypeptide under native conditions a Superdex 200 column is loaded with standard proteins of known molecular weight. Based on the elution volume of the standard proteins a calibration curve is plotted and the molecular weight of purified fusion polypeptide is determined. The FC-domain comprising CD137 receptor agonist fusion proteins elutes from the Superdex200 columns with an apparent molecular weight of approx. 140-180 kDa, which would confirm the homodimerization of the mature CD137 receptor agonist fusion polypeptide by the Fc domain.

Example 3: Trivalent Control Protein

[0161] To compare the relative binding between hexavalent CD137 receptor agonist fusion proteins and the, homo-trimeric trivalent CD137 receptor agonist fusion proteins stabilized with bacteriophage RB69-FOLDON is expressed in CHO-S cells and purified as described in the former section. The sequence is shown in the table below:

TABLE-US-00008 SEQ ID NO Sequence 38 METDTLLVFVLLVWVPAGNGQGMFAQLVAQNVLLIDGP (Trivalent LSWYSDPGLAGVSLTGGLSYKEDTKELVVAKAGVYYVF control FQLELRRVVAGEGSGSVSLALHLQPLRSAAGAAALALT protein) VDLPPASSEARNSAFGFQGRLLHLSAGQRLGVHLHTEA RARHAWQLTQGATVLGLFRVGSGSSGSSGSSGSGYIED APSDGKFYVRKDGAWVELPTASGPSSSSSSAWSHPQFE K.

Example 4: Determination of the In Vitro Stability of CD137 Receptor Agonist Proteins by Limited Protease Digestion

[0162] All CD137 receptor agonist proteins to be investigated will be expressed and purified as hexavalent Fc-Fusion protein as described in Example 1. The set will include CD137 receptor agonist proteins comprising the N297S mutation [according to the EU numbering system] in the CH2-domain and a hinge region that enables the formation of three disulfide bridges and additionally lack the upper hinge lysine [K223, according to the EU numbering system] which is mutated to glycine [K223G]. In a limited protease digestion assay, the aforementioned CD137 receptor agonist proteins comprising the N297S mutation and the K223G mutation simultaneously in context of a three disulfide enabling hinge will be compared to CD137 receptor agonist proteins comprising the N297S mutation but have the K223 wildtype present either in the context of a two disulfide or three disulfide enabling hinge region.

[0163] In addition CD137 receptor agonist proteins with the second linker element (iv) reduced to 4 amino-acids and the shortened hinge element (vi) will be investigated (e.g. SEQ ID NO: 32 and 34). Both engineering strategies (N297S combined with K223G mutation in context of a three disulfide enabling hinge region) and shortage of linker elements (iv and vi) have a potential impact on the stability of the respective molecules.

[0164] The stability of different CD137 agonistic proteins of the present invention can be addressed by limited protease digestion in vitro. For this analysis, the aforementioned CD137 receptor agonist proteins are incubated with low concentrations of proteases (e.g. Trypsin, V8 protease) at different temperatures (e.g. 4.degree. C., 25.degree. C., 37.degree. C.) for different amounts of time. Quantification of specific proteolytic fragments and their appearance over time can be subsequently measured by different methods, like SDS-PAGE, analytical SEC or analytical Mass-Spectrometry methods known in the art (e.g. Nano-RP-HPLC-ESI-MSMS). As the investigated proteins have most of their sequences in common, the faster appearance and enlarged quantities of specific proteolytic fragments from individual proteins over time can then be used to judge their relative stability and rank them to each other. With regard to protease based decoy kinetics of the aforementioned CD137 receptor agonist proteins investigated, the following order regarding their proteolytic stability is to be expected:

[0165] The CD137 receptor agonist proteins comprising the N297S and the K223G and the three disulfide enabling hinge region simultaneously have a prolonged stability as compared to the CD137 receptor agonist proteins comprising the N297S and wildtype K223 in the hinge region. The CD137 receptor agonist proteins comprising the SEQ ID NO: 21 as hinge linker have a prolonged stability as compared to CD137 receptor agonist proteins comprising the SEQ ID NO: 16 as hinge linker element.

Example 5: Stability/Aggregation Test

[0166] The contents of monomers and aggregates are determined by analytical SEC as described in Example 2. For this particular purpose the analysis is performed in buffers containing physiological salt concentrations at physiological pH (e.g. 0.9% NaCl, pH 7.4; PBS pH 7.4). A typical aggregation analysis is done on a Superdex200 column (GE Healthcare). This column separates proteins in the range between 10 to 800 kDa.

[0167] For determination of the apparent molecular weight of purified fusion polypeptide under native conditions a Superdex 200 column is loaded with standard proteins of known molecular weight. Based on the elution volume of the standard proteins a calibration curve is plotted and the apparent molecular weight of purified fusion proteins of unknown molecular weight is calculated based on the elution volume.

[0168] SEC analysis of soluble, non-aggregated protein typically shows a distinct single protein peak at a defined elution volume (measured at OD at 280 nm or at OD 214 nm). This elution volume corresponds to the apparent native molecular weight of the particular protein. With regard to the definition of "monomer" in the case of FC-fusion proteins, the assembly of two polypeptide-chains is driven by the FC-part of the protein and the functional unit is a protein consisting of two chains. This unit that contains two FC-linked polypeptide chains is defined as "monomer" in the case of Fc-fusion proteins regardless of being a dimerized single-chain fusion polypeptide.

[0169] If protein aggregation occurs, the SEC analysis shows additional protein peaks with lower retention volumes. Protein oligomers potentially serve as aggregation seeds and a high content of oligomers potentially leads to aggregation of the protein. Oligomers of large molecular weight and aggregates elute in the void volume of the Superdex200 column and cannot be analyzed by SEC with respect to their native molecular weight.

[0170] Purified preparations of CD137 receptor agonist fusion proteins should preferably contain only defined monomeric protein and only a very low amount of oligomeric protein. The degree of aggregation/oligomerization of a particular CD137 receptor agonist fusion protein preparation is determined on basis of the SEC analysis by calculating the peak areas of the OD280 diagram for the defined monomer and the oligomer/aggregate fraction, respectively. Based on the total peak area the percentage of defined monomer protein is calculated as follows:

monomer content [%]=[Peak area monomer protein]/[Total peak area].times.100)

Example 6: Determination of the Equilibrium Binding Constants for Tri- and Hexavalent CD137 Receptor Ligand Constructs by QCM Analysis

[0171] The equilibrium binding constants (K.sub.D) of trivalent and hexavalent constructs of CD137 receptor ligand are calculated based on kinetic binding data (k.sub.on and k.sub.off) that are determined with an automated biosensor system (Attana A100). The A100 allows to investigate molecular interactions in real-time based on the Quartz Crystal Microbalance (QCM) technique.

[0172] For this purpose the human CD137 receptor is immobilized to the surface of a carboxyl-activated QCM-chip. Subsequently the tri- or hexavalent CD137 receptor ligand, respectively, is used as an analyte at different concentrations (e.g. 0.5, 1, 2, 5, and 10 .mu.g/ml) for analyzing the kinetic binding data for ligand-receptor binding (k.sub.on) and dissociation (k.sub.off). The analysis is done in real time and the respective K.sub.D can be calculated:

K.sub.D=k.sub.off/k.sub.on.

[0173] The QCM analysis shows that the trivalent CD137 receptor ligand binds to the respective immobilized CD137 receptor with a KD in the low nM-range with an expected K.sub.D of 1-500 nM. However, hexavalent constructs of CD137 receptor ligand show a higher binding affinity in the pM-range towards the respective immobilized CD137 receptor with an expected K.sub.D of 1 pM-500 nM. A common characteristic of the kinetic binding data (k.sub.on and k.sub.off) is that the hexavalent constructs show faster k.sub.on in comparison to the trivalent constructs. In addition slower dissociation (k.sub.off) is commonly observed for the hexavalent ligands if compared to the trivalent ligand.

Example 7: T Cell Proliferation Assay

[0174] To assess the T cell activation capability of the CD137 receptor agonist, T cells are purified from human buffy coat preparations by negative selection using magnetic beads. Cells are labeled with CFSE and incubated with or without varying amounts of the CD137 receptor agonist and combined with an anti-human CD3 antibody for 2-5 days at 37.degree. C. Data on CFSE dilution as a means to measure cell division is acquired on a flow cytometer. IFN.gamma. production is measured by an ELISA assay using cell culture supernatants and an anti-human IFN.gamma. antibody for capture.

[0175] One expects to observe a clear augmentation of IFN.gamma. secretion by both CD4+ and CD8+ T cells when the CD137 receptor agonist is present in the T cell cultures along with the anti-human CD3 antibody. As well as higher IFN.gamma. production one expects to see more T cells to be driven into cell cycle by measuring CFSE dilution using flow cytometry. This would demonstrate a co-stimulatory effect of the CD137 receptor agonist in the context of T cell activation.

Example 8: CD137 Agonist Binding Assay

[0176] Primary, human T cells are isolated from fresh buffy coat preparations using negative selection and magnetic beads. Cells are seeded into 24-well plates at 2.times.10e6 cells per well. T cells are incubated with an anti-human CD3 antibody (clone HIT3a, 1 .mu.g/ml), anti-human CD28 antibody (clone CD28.2, 5 .mu.g/ml) and varying amounts of Protein A (CD137L, 10-1000 ng/ml) or simply left in medium as control. After 3 days at 37.degree. C. cells are fluorescently labeled with anti-human CD137 and anti-human CD4 or anti-human CD8 antibodies. CD137 fluorescence is assessed on a guava easyCyte flow cytometer within CD4+ and CD8+ T cell populations.

[0177] When comparing T cell populations incubated with anti-CD3 and anti-CD28 antibodies to control cells left in medium alone, one expects to observe a lower fluorescent signal for CD137 indicating an activation-induced downregulation of the receptor. This effect can be stronger and dose-dependent, when cells are co-incubated with the CD137 agonist (Protein A), which indicates a supplementary effect caused by the CD137 agonist (Protein A). Such results would suggest a binding of the CD137 agonist (Protein A) to its receptor in vitro.

Example 9: Human In Vitro T Cell Proliferation Assay

[0178] Total T cells (human) purified by negative selection and magnetic beads (pan T cell isolation kit, Miltenyi Biotec) from the peripheral blood of healthy donors and stained with CFSE (CellTrace.TM. CFSE Cell Proliferation Kit, for flow cytometry, ThermoFisher) and seeded into 24-well plates at 2.times.10e6 cells per well. Cells were incubated at 37.degree. C. for 5 days with media alone, soluble anti-CD3 antibody (clone OKT3 at 1 .mu.g/ml) alone, anti-CD3 antibody plus anti-CD28 antibody (clone 28.2 at 1 .mu.g/ml) or anti-CD3 antibody plus mature Protein A (SEQ ID NO: 27) at 10, 100 or 1000 ng/ml, respectively.

[0179] On day 5, cells were washed and stained with DAPI (to exclude dead cells) and specific antibodies. Expression of Forward Scatter (FSC or size) and proliferation dependent CFSE dilution was measured by flow cytometry with a Guava EasyCyte 12 Flow Cytometer (EMD Millipore). Data analysis was performed on a minimum of ten thousand recorded events per sample with FlowJo 10.1 software (FlowJo, LLC). The percentage of responding cells was determined by gating on Forward Scatter and CFSE using the media control to determine proper gate location. Cells that had either increased cell size or decreased CFSE levels were labeled as responding cells. The individual data from two biological replicates from one donor is shown in below copied table (Quantification of T cell activation) These results are consistent with results from additional donors and clearly showed that treatment of human T cells in vitro with Protein A enhances T cell activation and proliferation as compared to antibody stimulation alone.

[0180] Quantification of T Cell Activation.

TABLE-US-00009 Human T cell activation following treatment with Protein A in vitro % of cells responding Stimulation Sample 1 Sample 2 Media 3 3 anti-CD3 56 62 anti-CD3/28 87 85 anti-CD3 + APG1472 10 ng/ml 71 69 anti-CD3 + APG1472 100 ng/ml 75 71 anti-CD3 + APG1472 1000 ng/ml 66 75

Example 10: Receptor Binding Assay

[0181] For ELISA assays assessing functional binding of CD137 receptor agonist protein of the invention to its corresponding receptor, coating of microtiter plates was performed with 1 .mu.g/ml CD137-Fc (Bio-Techne GmbH, Wiesbaden-Nordenstadt, Germany). After blocking with StartingBlock (Life Technologies GmbH, Darmstadt, Germany), wells were incubated with indicated concentrations of strep-tagged Protein A (SEQ ID NO: 28). Binding to its corresponding receptor was detected via its Strep Tag II employing the anti-StrepTag-peroxidase StrepTactin-HRP (1:5000, IBA GmbH, Goettingen, Germany) and subsequent detection of the converted Peroxidase-substrate TMB one (Kem-En-Tec Diagnostics, Taastrup, Denmark) at a wavelength of 450 nm in an ELISA reader. FIG. 6 clearly depicts concentration dependent binding of Protein A to its receptor.

Sequence CWU 1

1

431254PRTHomo sapiensCD137 ligand WT 1Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro1 5 10 15Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 20 25 30Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 35 40 45Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 50 55 60Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp65 70 75 80Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 85 90 95Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 100 105 110Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 115 120 125Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 130 135 140Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser145 150 155 160Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 165 170 175Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 180 185 190Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 195 200 205Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 210 215 220Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val225 230 235 240Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 245 25028PRTArtificial Sequencelinker 2Gly Ser Gly Ser Gly Asn Gly Ser1 538PRTArtificial Sequencelinker 3Gly Ser Gly Ser Gly Ser Gly Ser1 548PRTArtificial Sequencelinker 4Gly Gly Ser Gly Ser Gly Ser Gly1 556PRTArtificial Sequencelinker 5Gly Gly Ser Gly Ser Gly1 564PRTArtificial Sequencelinker 6Gly Gly Ser Gly178PRTArtificial Sequencelinker 7Gly Gly Ser Gly Asn Gly Ser Gly1 588PRTArtificial Sequencelinker 8Gly Gly Asn Gly Ser Gly Ser Gly1 596PRTArtificial Sequencelinker 9Gly Gly Asn Gly Ser Gly1 5106PRTArtificial Sequencelinker 10Gly Ser Gly Ser Gly Ser1 5114PRTArtificial Sequencelinker 11Gly Ser Gly Ser1123PRTArtificial Sequencelinker 12Gly Ser Gly113218PRTArtificial SequenceIGG1-Fc N297S 13Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro1 5 10 15Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 20 25 30Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 35 40 45Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 50 55 60Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu65 70 75 80His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 85 90 95Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 100 105 110Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 115 120 125Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 130 135 140Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn145 150 155 160Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 165 170 175Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 180 185 190Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 195 200 205Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 21514217PRTArtificial SequenceIGG1-Fc WT 14Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys1 5 10 15Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 20 25 30Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 35 40 45Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 50 55 60Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His65 70 75 80Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 85 90 95Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 100 105 110Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 115 120 125Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 130 135 140Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn145 150 155 160Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 165 170 175Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 180 185 190Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 195 200 205Lys Ser Leu Ser Leu Ser Pro Gly Lys 210 21515745PRTArtificial SequencePROT A (CD137L Deglyco Fc) 15Met Glu Thr Asp Thr Leu Leu Val Phe Val Leu Leu Val Trp Val Pro1 5 10 15Ala Gly Asn Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 20 25 30Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 35 40 45Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 50 55 60Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu65 70 75 80Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 85 90 95Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 100 105 110Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 115 120 125Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 130 135 140Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu145 150 155 160Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 165 170 175Gly Asn Gly Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 180 185 190Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 195 200 205Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 210 215 220Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu225 230 235 240Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 245 250 255Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 260 265 270Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 275 280 285Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 290 295 300Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu305 310 315 320Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 325 330 335Gly Asn Gly Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 340 345 350Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 355 360 365Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 370 375 380Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu385 390 395 400Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 405 410 415Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 420 425 430Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 435 440 445Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 450 455 460Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu465 470 475 480Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser 485 490 495Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro 500 505 510Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 515 520 525Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 530 535 540Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn545 550 555 560Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 565 570 575Glu Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 580 585 590Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 595 600 605Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 610 615 620Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu625 630 635 640Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 645 650 655Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 660 665 670Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 675 680 685Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 690 695 700Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr705 710 715 720Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Ser Ser Ser Ser Ser Ser 725 730 735Ala Trp Ser His Pro Gln Phe Glu Lys 740 7451621PRTArtificial Sequencehinge linker 16Gly Ser Ser Ser Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His1 5 10 15Thr Cys Pro Pro Cys 201720PRTArtificial Sequenceuniversal signal peptide 17Met Glu Thr Asp Thr Leu Leu Val Phe Val Leu Leu Val Trp Val Pro1 5 10 15Ala Gly Asn Gly 201815PRTArtificial Sequenceserine linker with strep tag 18Ser Ser Ser Ser Ser Ser Ala Trp Ser His Pro Gln Phe Glu Lys1 5 10 151920PRTArtificial Sequencehinge linker 19Gly Ser Ser Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr1 5 10 15Cys Pro Pro Cys 202019PRTArtificial Sequencehinge linker 20Gly Ser Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys1 5 10 15Pro Pro Cys2118PRTArtificial Sequencehinge linker 21Gly Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro1 5 10 15Pro Cys2216PRTArtificial Sequencehinge linker 22Gly Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys1 5 10 152318PRTArtificial Sequencehinge linker 23Gly Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys1 5 10 15Gly Ser2420PRTArtificial Sequencehinge linker 24Gly Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys1 5 10 15Gly Ser Gly Ser 2025731PRTArtificial SequencePROTEIN A - no strep tag 25Met Glu Thr Asp Thr Leu Leu Val Phe Val Leu Leu Val Trp Val Pro1 5 10 15Ala Gly Asn Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 20 25 30Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 35 40 45Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 50 55 60Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu65 70 75 80Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 85 90 95Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 100 105 110Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 115 120 125Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 130 135 140Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu145 150 155 160Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 165 170 175Gly Asn Gly Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 180 185 190Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 195 200 205Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 210 215 220Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu225 230 235 240Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 245 250 255Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 260 265 270Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 275 280 285Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 290 295 300Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu305 310 315 320Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 325 330 335Gly Asn Gly Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 340 345 350Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 355 360 365Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 370 375 380Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu385 390 395 400Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 405 410 415Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 420 425 430Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 435 440 445Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 450 455 460Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu465 470 475 480Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser 485 490 495Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro 500 505 510Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro 515 520 525Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr 530 535 540Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn545 550 555 560Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg 565 570 575Glu Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val 580 585 590Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser 595 600 605Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 610 615 620Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu625 630 635 640Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 645 650 655Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 660 665 670Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 675 680

685Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 690 695 700Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr705 710 715 720Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 725 73026730PRTArtificial SequenceCD137L-wt fused to Seq_14 26Met Glu Thr Asp Thr Leu Leu Val Phe Val Leu Leu Val Trp Val Pro1 5 10 15Ala Gly Asn Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 20 25 30Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 35 40 45Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 50 55 60Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu65 70 75 80Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 85 90 95Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 100 105 110Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 115 120 125Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 130 135 140Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu145 150 155 160Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 165 170 175Gly Asn Gly Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 180 185 190Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 195 200 205Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 210 215 220Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu225 230 235 240Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 245 250 255Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 260 265 270Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 275 280 285Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 290 295 300Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu305 310 315 320Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 325 330 335Gly Asn Gly Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 340 345 350Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 355 360 365Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 370 375 380Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu385 390 395 400Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 405 410 415Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 420 425 430Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 435 440 445Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 450 455 460Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu465 470 475 480Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser 485 490 495Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro 500 505 510Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro 515 520 525Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 530 535 540Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp545 550 555 560Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 565 570 575Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 580 585 590His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 595 600 605Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 610 615 620Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu625 630 635 640Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 645 650 655Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 660 665 670Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 675 680 685Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 690 695 700Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr705 710 715 720Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 725 73027711PRTArtificial SequenceCD137L-wt fused to Seq_13 (no sig, no strep tag, no glyco) 27Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp1 5 10 15Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 20 25 30Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 35 40 45Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 50 55 60Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln65 70 75 80Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 85 90 95Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 100 105 110Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 115 120 125His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 130 135 140Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser145 150 155 160Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 165 170 175Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 180 185 190Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 195 200 205Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 210 215 220Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln225 230 235 240Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 245 250 255Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 260 265 270Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 275 280 285His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 290 295 300Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser305 310 315 320Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 325 330 335Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 340 345 350Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 355 360 365Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 370 375 380Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln385 390 395 400Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 405 410 415Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 420 425 430Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 435 440 445His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 450 455 460Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser Ser Ser Ser465 470 475 480Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 485 490 495Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 500 505 510Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 515 520 525Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 530 535 540Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr545 550 555 560Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 565 570 575Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 580 585 590Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 595 600 605Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 610 615 620Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp625 630 635 640Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 645 650 655Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 660 665 670Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 675 680 685Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 690 695 700Leu Ser Leu Ser Pro Gly Lys705 71028725PRTArtificial SequenceCD137L-wt fused to Seq_13 (no sig, no glyco) 28Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp1 5 10 15Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 20 25 30Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 35 40 45Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 50 55 60Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln65 70 75 80Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 85 90 95Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 100 105 110Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 115 120 125His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 130 135 140Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser145 150 155 160Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 165 170 175Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 180 185 190Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 195 200 205Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 210 215 220Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln225 230 235 240Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 245 250 255Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 260 265 270Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 275 280 285His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 290 295 300Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser305 310 315 320Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 325 330 335Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 340 345 350Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 355 360 365Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 370 375 380Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln385 390 395 400Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 405 410 415Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 420 425 430Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 435 440 445His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 450 455 460Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser Ser Ser Ser465 470 475 480Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 485 490 495Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 500 505 510Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 515 520 525Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 530 535 540Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr545 550 555 560Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 565 570 575Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 580 585 590Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 595 600 605Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 610 615 620Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp625 630 635 640Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 645 650 655Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 660 665 670Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 675 680 685Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 690 695 700Leu Ser Leu Ser Pro Gly Ser Ser Ser Ser Ser Ser Ala Trp Ser His705 710 715 720Pro Gln Phe Glu Lys 72529710PRTArtificial Sequenceexample 29Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp1 5 10 15Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 20 25 30Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 35 40 45Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 50 55 60Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln65 70 75 80Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 85 90 95Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 100 105 110Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 115 120 125His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 130 135 140Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser145 150 155 160Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 165 170 175Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 180 185 190Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 195 200 205Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 210 215 220Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala

Leu His Leu Gln225 230 235 240Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 245 250 255Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 260 265 270Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 275 280 285His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 290 295 300Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser305 310 315 320Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 325 330 335Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 340 345 350Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 355 360 365Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 370 375 380Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln385 390 395 400Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 405 410 415Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 420 425 430Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 435 440 445His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 450 455 460Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser Ser Ser Ser465 470 475 480Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 485 490 495Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 500 505 510Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 515 520 525Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 530 535 540Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn545 550 555 560Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 565 570 575Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 580 585 590Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 595 600 605Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 610 615 620Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile625 630 635 640Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 645 650 655Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 660 665 670Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 675 680 685Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 690 695 700Ser Leu Ser Pro Gly Lys705 71030716PRTArtificial Sequenceexample 30Gln Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu1 5 10 15Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 20 25 30Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 35 40 45Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 50 55 60Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu65 70 75 80His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 85 90 95Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 100 105 110Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 115 120 125Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 130 135 140Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly145 150 155 160Asn Gly Ser Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln 165 170 175Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly 180 185 190Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr 195 200 205Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln 210 215 220Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser225 230 235 240Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala 245 250 255Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn 260 265 270Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln 275 280 285Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp 290 295 300Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser305 310 315 320Gly Ser Gly Asn Gly Ser Asp Leu Arg Gln Gly Met Phe Ala Gln Leu 325 330 335Val Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser 340 345 350Asp Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys 355 360 365Glu Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val 370 375 380Phe Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly385 390 395 400Ser Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly 405 410 415Ala Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu 420 425 430Ala Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser 435 440 445Ala Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg 450 455 460His Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg465 470 475 480Val Gly Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro 485 490 495Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 500 505 510Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 515 520 525Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 530 535 540Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro545 550 555 560Arg Glu Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr 565 570 575Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 580 585 590Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 595 600 605Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 610 615 620Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly625 630 635 640Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 645 650 655Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 660 665 670Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 675 680 685Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 690 695 700Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys705 710 71531720PRTArtificial Sequenceexample 31Gln Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu1 5 10 15Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 20 25 30Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 35 40 45Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 50 55 60Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu65 70 75 80His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 85 90 95Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 100 105 110Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 115 120 125Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 130 135 140Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly145 150 155 160Asn Gly Ser Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln 165 170 175Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly 180 185 190Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr 195 200 205Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln 210 215 220Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser225 230 235 240Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala 245 250 255Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn 260 265 270Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln 275 280 285Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp 290 295 300Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser305 310 315 320Gly Ser Gly Asn Gly Ser Asp Leu Arg Gln Gly Met Phe Ala Gln Leu 325 330 335Val Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser 340 345 350Asp Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys 355 360 365Glu Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val 370 375 380Phe Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly385 390 395 400Ser Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly 405 410 415Ala Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu 420 425 430Ala Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser 435 440 445Ala Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg 450 455 460His Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg465 470 475 480Val Gly Ser Ser Ser Ser Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr 485 490 495His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 500 505 510Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 515 520 525Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 530 535 540Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala545 550 555 560Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val 565 570 575Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 580 585 590Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 595 600 605Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 610 615 620Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys625 630 635 640Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 645 650 655Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 660 665 670Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 675 680 685Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 690 695 700Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys705 710 715 72032711PRTArtificial Sequenceexample 32Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile1 5 10 15Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser 20 25 30Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val 35 40 45Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg 50 55 60Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu65 70 75 80Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val 85 90 95Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe 100 105 110Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His 115 120 125Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly 130 135 140Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly145 150 155 160Ser Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu 165 170 175Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val 180 185 190Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val 195 200 205Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg 210 215 220Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His225 230 235 240Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr 245 250 255Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly 260 265 270Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val 275 280 285His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln 290 295 300Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn305 310 315 320Gly Ser Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu 325 330 335Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 340 345 350Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 355 360 365Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 370 375 380Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu385 390 395 400His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 405 410 415Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 420 425 430Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 435 440 445Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 450 455 460Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser465 470 475 480Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 485 490 495Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 500 505 510Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 515 520 525Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 530 535 540Gly Val Glu Val His

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr545 550 555 560Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 565 570 575Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 580 585 590Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 595 600 605Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 610 615 620Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp625 630 635 640Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 645 650 655Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 660 665 670Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 675 680 685Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 690 695 700Leu Ser Leu Ser Pro Gly Lys705 71033714PRTArtificial Sequenceexample 33Gln Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile1 5 10 15Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser 20 25 30Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val 35 40 45Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg 50 55 60Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu65 70 75 80Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val 85 90 95Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe 100 105 110Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His 115 120 125Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly 130 135 140Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly145 150 155 160Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu 165 170 175Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val 180 185 190Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val 195 200 205Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg 210 215 220Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His225 230 235 240Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr 245 250 255Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly 260 265 270Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val 275 280 285His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln 290 295 300Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn305 310 315 320Gly Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu 325 330 335Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 340 345 350Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 355 360 365Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 370 375 380Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu385 390 395 400His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 405 410 415Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 420 425 430Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 435 440 445Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 450 455 460Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser465 470 475 480Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 485 490 495Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 500 505 510Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 515 520 525Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 530 535 540Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu545 550 555 560Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 565 570 575His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 580 585 590Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 595 600 605Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 610 615 620Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr625 630 635 640Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 645 650 655Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 660 665 670Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 675 680 685Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 690 695 700Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys705 71034714PRTArtificial Sequenceexample 34Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile1 5 10 15Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser 20 25 30Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val 35 40 45Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg 50 55 60Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu65 70 75 80Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val 85 90 95Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe 100 105 110Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His 115 120 125Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly 130 135 140Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly145 150 155 160Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu 165 170 175Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val 180 185 190Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val 195 200 205Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg 210 215 220Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His225 230 235 240Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr 245 250 255Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly 260 265 270Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val 275 280 285His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln 290 295 300Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn305 310 315 320Gly Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu 325 330 335Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 340 345 350Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 355 360 365Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 370 375 380Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu385 390 395 400His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 405 410 415Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 420 425 430Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 435 440 445Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 450 455 460Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser465 470 475 480Ser Ser Ser Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 485 490 495Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 500 505 510Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 515 520 525Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 530 535 540Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu545 550 555 560Glu Gln Tyr Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 565 570 575His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 580 585 590Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 595 600 605Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 610 615 620Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr625 630 635 640Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 645 650 655Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 660 665 670Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 675 680 685Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 690 695 700Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys705 71035711PRTArtificial SequenceFAKE SEQ --> PLEASE REPLACE BY CORRECT SEQ 35Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp1 5 10 15Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 20 25 30Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 35 40 45Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 50 55 60Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln65 70 75 80Pro Leu Arg Ser Ala Asn Gly Ser Ala Ala Leu Ala Leu Thr Val Asp 85 90 95Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 100 105 110Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 115 120 125His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 130 135 140Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser145 150 155 160Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 165 170 175Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 180 185 190Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 195 200 205Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 210 215 220Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln225 230 235 240Pro Leu Arg Ser Ala Asn Gly Ser Ala Ala Leu Ala Leu Thr Val Asp 245 250 255Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 260 265 270Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 275 280 285His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 290 295 300Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser305 310 315 320Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 325 330 335Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 340 345 350Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 355 360 365Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 370 375 380Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln385 390 395 400Pro Leu Arg Ser Ala Asn Gly Ser Ala Ala Leu Ala Leu Thr Val Asp 405 410 415Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 420 425 430Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 435 440 445His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 450 455 460Thr Val Leu Gly Leu Phe Arg Val Gly Ser Ser Ser Ser Ser Ser Ser465 470 475 480Ser Gly Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 485 490 495Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 500 505 510Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 515 520 525Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 530 535 540Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr545 550 555 560Ser Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 565 570 575Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 580 585 590Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 595 600 605Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys 610 615 620Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp625 630 635 640Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 645 650 655Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 660 665 670Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 675 680 685Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 690 695 700Leu Ser Leu Ser Pro Gly Lys705 71036472PRTArtificial Sequenceexample of scCD137L-RBD module 36Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp1 5 10 15Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 20 25 30Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 35 40 45Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 50 55 60Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln65 70 75 80Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 85 90 95Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 100 105 110Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 115 120 125His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 130 135 140Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser

Gly Asn Gly Ser145 150 155 160Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 165 170 175Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 180 185 190Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 195 200 205Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 210 215 220Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln225 230 235 240Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 245 250 255Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 260 265 270Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 275 280 285His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 290 295 300Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser305 310 315 320Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 325 330 335Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 340 345 350Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 355 360 365Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 370 375 380Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln385 390 395 400Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val Asp 405 410 415Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 420 425 430Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 435 440 445His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 450 455 460Thr Val Leu Gly Leu Phe Arg Val465 470372240DNAArtificial SequenceDNA sequence encoding SEQ ID NO25 37aagctttagg gataacaggg taatagccgc caccatggag actgacaccc tgctggtgtt 60cgtgctgctg gtctgggtgc ctgcaggaaa tggacagggc atgttcgctc aactggtcgc 120acagaacgtg ctgctcattg acggtcccct gtcttggtac tccgatccag ggttggcagg 180agtgtccttg acaggagggc tgtcctataa ggaggatacc aaagagctgg tggtagcaaa 240ggctggtgtg tattacgtgt tctttcagct ggagctgcgc agagtcgtcg caggcgaagg 300atctggtagt gtgtcactgg cactgcactt gcagcccctt cggtccgctg ccggggcagc 360agcactggcc ctgaccgtcg atctgccacc cgcttctagc gaggcacgaa actcagcctt 420tgggtttcag ggtcgcctgc tgcacctgag cgccggacag aggctgggcg ttcatctgca 480caccgaggcc agagccagac acgcttggca gttgactcag ggagctacgg tcctcggtct 540gtttcgagta ggcagcggaa gcggcaatgg ctctcagggc atgtttgctc agctggtagc 600ccagaacgta ctcctgatcg atggccctct ttcatggtac tcagaccccg gactggccgg 660agttagcctt acaggtgggc ttagttataa ggaggacaca aaggaattgg ttgtggccaa 720agctggcgtg tactatgtgt tcttccagct tgagctccgc agagtcgtgg ctggggaggg 780ctctggcagt gtgagccttg cccttcatct gcaacctttg cggagcgcag ccggcgctgc 840tgcactggcc cttacagtgg atttgccacc cgcaagtagt gaagctcgca attccgcatt 900cggtttccag ggccgtctgc tccatctttc tgccggtcaa cgtctgggag ttcacctcca 960cactgaggct agggccaggc atgcttggca gctgactcaa ggagccactg tcttgggact 1020ctttcgggta ggctccgggt ctggcaacgg ctcccagggg atgtttgccc aactggtcgc 1080ccagaatgtc ctgctcatcg atggtcctct gagctggtat tccgaccctg gactggctgg 1140tgtgagcctg actggcggac tctcctacaa agaggacacc aaggaactgg tggtggccaa 1200agccggggtg tactacgtgt tcttccagtt ggaactgcgg cgggttgtgg ctggcgaggg 1260atcaggttcc gttagtctgg ccctgcacct ccagcctctg aggtctgctg ctggtgccgc 1320cgctctggcc ttgaccgtcg acctcccacc cgcatcttcc gaagcccgaa attcagcctt 1380cgggttccag ggcagactgc tgcatctgag tgctggacag cgccttgggg ttcatctcca 1440caccgaagcc agggcccgac atgcctggca gctcacacaa ggcgcaaccg ttttggggct 1500ctttcgtgtg ggatcctcga gttcatcgtc ctcatccggc tcatgtgata agacccacac 1560ctgccctccc tgtcctgccc ctgagctgct gggcggacct tctgtgttcc tgttcccccc 1620caagcctaag gacaccctga tgatctccag gacccctgag gtgacctgtg tggtggtgga 1680cgtgtctcac gaagatcccg aggtgaagtt caactggtac gtggacggcg tggaggtcca 1740caacgccaag accaagccta gggaggagca gtacagctcc acctaccggg tggtgtctgt 1800gctgaccgtg ctgcaccagg attggctgaa cggaaaggag tataagtgta aggtctccaa 1860caaggccctg cctgccccca tcgagaaaac catctccaag gccaagggcc agcctcggga 1920gcctcaggtg tacaccctgc ctcctagcag ggaggagatg accaagaacc aggtgtccct 1980gacctgtctg gtgaagggct tctacccttc cgatatcgcc gtggagtggg agtctaatgg 2040ccagcccgag aacaactaca agaccacccc tcctgtgctg gactctgacg gctccttctt 2100cctgtactcc aagctgaccg tggacaagtc cagatggcag cagggcaacg tgttctcctg 2160ctccgtgatg cacgaggccc tgcacaatca ctacacccag aagtccctgt ctctgagtcc 2220gggcaagtaa taggcgcgcc 224038229PRTArtificial Sequencehomotrimeric CD137L fused to RB69 FOLDON 38Met Glu Thr Asp Thr Leu Leu Val Phe Val Leu Leu Val Trp Val Pro1 5 10 15Ala Gly Asn Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val 20 25 30Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala 35 40 45Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu 50 55 60Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu65 70 75 80Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala 85 90 95Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala 100 105 110Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala 115 120 125Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu 130 135 140Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu145 150 155 160Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser 165 170 175Ser Gly Ser Ser Gly Ser Ser Gly Ser Gly Tyr Ile Glu Asp Ala Pro 180 185 190Ser Asp Gly Lys Phe Tyr Val Arg Lys Asp Gly Ala Trp Val Glu Leu 195 200 205Pro Thr Ala Ser Gly Pro Ser Ser Ser Ser Ser Ser Ala Trp Ser His 210 215 220Pro Gln Phe Glu Lys22539481PRTArtificial Sequencesingle chain CD137 lignad RBD (example) 39Gln Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu1 5 10 15Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 20 25 30Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 35 40 45Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 50 55 60Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu65 70 75 80His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 85 90 95Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 100 105 110Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 115 120 125Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 130 135 140Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly145 150 155 160Asn Gly Ser Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln 165 170 175Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly 180 185 190Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr 195 200 205Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln 210 215 220Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser225 230 235 240Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala 245 250 255Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn 260 265 270Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln 275 280 285Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp 290 295 300Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser305 310 315 320Gly Ser Gly Asn Gly Ser Asp Leu Arg Gln Gly Met Phe Ala Gln Leu 325 330 335Val Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser 340 345 350Asp Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys 355 360 365Glu Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val 370 375 380Phe Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly385 390 395 400Ser Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly 405 410 415Ala Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu 420 425 430Ala Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser 435 440 445Ala Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg 450 455 460His Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg465 470 475 480Val40475PRTArtificial Sequencesingle chain CD137 lignad RBD (example) 40Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile1 5 10 15Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser 20 25 30Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val 35 40 45Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg 50 55 60Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu65 70 75 80Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val 85 90 95Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe 100 105 110Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His 115 120 125Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly 130 135 140Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly145 150 155 160Ser Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu 165 170 175Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val 180 185 190Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val 195 200 205Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg 210 215 220Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His225 230 235 240Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr 245 250 255Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly 260 265 270Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val 275 280 285His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln 290 295 300Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn305 310 315 320Gly Ser Arg Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu 325 330 335Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 340 345 350Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 355 360 365Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 370 375 380Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu385 390 395 400His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 405 410 415Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 420 425 430Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 435 440 445Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 450 455 460Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val465 470 47541475PRTArtificial Sequencesingle chain CD137 lignad RBD (example) 41Gln Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile1 5 10 15Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser 20 25 30Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val 35 40 45Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg 50 55 60Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu65 70 75 80Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val 85 90 95Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe 100 105 110Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His 115 120 125Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly 130 135 140Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly145 150 155 160Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu 165 170 175Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val 180 185 190Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val 195 200 205Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg 210 215 220Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His225 230 235 240Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr 245 250 255Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly 260 265 270Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val 275 280 285His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln 290 295 300Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn305 310 315 320Gly Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu 325 330 335Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 340 345 350Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 355 360 365Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 370 375 380Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu385 390 395 400His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 405 410 415Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 420 425 430Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 435 440 445Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 450 455 460Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val465 470 47542475PRTArtificial Sequencesingle chain CD137 lignad RBD (example) 42Ser Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile1 5 10 15Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser 20 25 30Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val 35 40 45Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg 50 55

60Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu65 70 75 80Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr Val 85 90 95Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe 100 105 110Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His 115 120 125Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly 130 135 140Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly145 150 155 160Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu 165 170 175Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val 180 185 190Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val 195 200 205Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg 210 215 220Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His225 230 235 240Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu Thr 245 250 255Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly 260 265 270Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val 275 280 285His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln 290 295 300Gly Ala Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn305 310 315 320Gly Ser Gly Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu 325 330 335Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly 340 345 350Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu 355 360 365Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu 370 375 380Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu385 390 395 400His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala Ala Ala Leu Ala Leu 405 410 415Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe 420 425 430Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly 435 440 445Val His Leu His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr 450 455 460Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val465 470 47543472PRTArtificial Sequencesingle chain CD137 lignad RBD (example) 43Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp1 5 10 15Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 20 25 30Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 35 40 45Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 50 55 60Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln65 70 75 80Pro Leu Arg Ser Ala Asn Gly Ser Ala Ala Leu Ala Leu Thr Val Asp 85 90 95Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 100 105 110Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 115 120 125His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 130 135 140Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser145 150 155 160Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 165 170 175Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 180 185 190Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 195 200 205Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 210 215 220Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln225 230 235 240Pro Leu Arg Ser Ala Asn Gly Ser Ala Ala Leu Ala Leu Thr Val Asp 245 250 255Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 260 265 270Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 275 280 285His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 290 295 300Thr Val Leu Gly Leu Phe Arg Val Gly Ser Gly Ser Gly Asn Gly Ser305 310 315 320Gln Gly Met Phe Ala Gln Leu Val Ala Gln Asn Val Leu Leu Ile Asp 325 330 335Gly Pro Leu Ser Trp Tyr Ser Asp Pro Gly Leu Ala Gly Val Ser Leu 340 345 350Thr Gly Gly Leu Ser Tyr Lys Glu Asp Thr Lys Glu Leu Val Val Ala 355 360 365Lys Ala Gly Val Tyr Tyr Val Phe Phe Gln Leu Glu Leu Arg Arg Val 370 375 380Val Ala Gly Glu Gly Ser Gly Ser Val Ser Leu Ala Leu His Leu Gln385 390 395 400Pro Leu Arg Ser Ala Asn Gly Ser Ala Ala Leu Ala Leu Thr Val Asp 405 410 415Leu Pro Pro Ala Ser Ser Glu Ala Arg Asn Ser Ala Phe Gly Phe Gln 420 425 430Gly Arg Leu Leu His Leu Ser Ala Gly Gln Arg Leu Gly Val His Leu 435 440 445His Thr Glu Ala Arg Ala Arg His Ala Trp Gln Leu Thr Gln Gly Ala 450 455 460Thr Val Leu Gly Leu Phe Arg Val465 470

Claims

1. (canceled)

2. A CD137 receptor agonist protein comprising a single-chain fusion polypeptide comprising: (i) a first soluble CD137L domain, (ii) a first peptide linker, (iii) a second soluble CD137L domain, (iv) a second peptide linker, and (v) a third soluble CD137L domain, and (vi) an antibody derived Fab- or Fc-fragment, wherein the CD137L domains (i), (iii) and (v) are N- and C-terminally shortened domains consisting of amino acids starting at an amino acid between 86 and 90 and ending at amino acid 240, or 241, or 243 of SEQ ID NO:1.

3. The CD137 receptor agonist protein of claim 2, wherein the Fc fragment of (vi) consists of the amino acid sequence as shown in SEQ ID NO: 13 or 14 or amino acids 1-217 of SEQ ID NO: 13 or 14.

4. The CD137 receptor agonist protein of claim 2, wherein the Fc fragment of (vi) is fused to the C-terminal end of the third CD137L domain (v) via a hinge-linker of any one of SEQ ID NOS: 16 or 19-24.

5. The CD137 receptor agonist protein of claim 2, wherein the Fab fragment of (vi) is fused to the N-terminal end of the first CD137L domain.

6. The CD137 receptor agonist protein of claim 5, wherein antibody Fab fragment of (vi) is fused to the N-terminal end of the first CD137L domain via short G/C rich linkers according to SEQ ID NOS: 2-12.

7. The CD137 receptor agonist protein of claim 2, wherein in at least one of the soluble CD137L domains, the starting amino acid D86 or R88 or Q89 is replaced by a neutral amino acid.

8. The CD137 receptor agonist protein of claim 2, wherein the soluble CD137L domains optionally comprises a mutation at position D86, L87, R88, Q89, D112, V118, A154, A174, A176, A188, T241, or at two or more of said positions.

9. The CD137 receptor agonist protein of claim 2, wherein the first and second peptide linkers (ii) and (iv) independently have a length of 3-8 amino acids.

10. The CD137 receptor agonist protein of claim 2, wherein the first and the second peptide linkers (ii) and (iv) consist of the amino acid sequence according to SEQ ID NO: 2.

11. The CD137 receptor agonist protein of claim 2, which additionally comprises an N-terminal signal peptide domain.

12. The CD137 receptor agonist protein of claim 2, wherein the polypeptide is further post-translationally modified.

13. The CD137 receptor agonist protein of claim 4, wherein the polypeptide is further post-translationally modified.

14. The CD137 receptor agonist protein of claim 13, wherein the post-translational modification comprises modification of the N-terminal glutamine to pyroglutamate.

15. A nucleic acid molecule encoding the CD137 receptor agonist protein of claim 2, in an operative linkage with an expression control sequence.

16. An expression vector comprising the nucleic acid molecule of claim 15.

17. A cell or a non-human organism transformed or transfected with the nucleic acid molecule of claim 15.

18. A pharmaceutical or diagnostic composition comprising as an active agent the CD137 receptor agonist protein of claim 2 and one or more pharmaceutically acceptable carriers, diluents, excipients and/or adjuvants.