METHODS OF CONTROLLING SEED SIZE IN PLANTS

Abstract

This invention relates to the identification of a regulator protein (termed CYP78A6, or EOD3) which controls the size of plant seeds and organs in Arabidopsis and other plants. Manipulation of CYP78A protein expression may useful, for example, in improving crop yield and increasing plant biomass.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This is a divisional application of U.S. Ser. No. 16/628,101, filed Jun. 20, 2017, which is a divisional application of U.S. Ser. No. 14/372,582 filed Jul. 16, 2014, which is a National Phase application claiming priority to PCT/GB2013/050072 filed Jan. 15, 2013, which claims priority to GB 1202258.8 filed Feb. 9, 2012 and U.S. Provisional Application Ser. No. 61/588,792 filed Jan. 20, 2012, all of which are herein incorporated by reference in their entirety.

FIELD OF INVENTION

[0002] This invention relates to the control of the size of the seeds which are produced by plants.

BACKGROUND OF THE INVENTIONS

[0003] Seed size is a key determinant of evolutionary fitness in plants and is also an important agronomic trait during crop domestication (Orsi and Tanksley, 2009). Several studies suggest that seedlings of large-seeded plants are better able to tolerate many of the stresses encountered during seedling establishment, whereas small-seeded plants are considered to have superior colonization abilities because they produce large numbers of seeds (Westoby et al., 2002; Moles et al., 2005). At the same time, seed size is negatively associated with the number of seeds produced by a plant due to the limited resources of the mother plant (Harper et al., 1970). Scientific interest in seed size relates not only to its importance in plant fitness, but also to crop domestication. Crops domesticated for consumption of their seeds (e.g. rice and wheat) often produce seeds significantly larger than their wild ancestors (Fan et al., 2006; Song et al., 2007; Gegas et al., 2010).

[0004] A seed consists of three major components; the embryo, the endosperm and the seed coat, that originate from different cells of the ovule and possess different complements of maternal and paternal genomes. In angiosperms, seed development involves a double fertilization process in which one sperm nucleus fuses with the egg to produce the diploid embryo, while the other sperm nucleus fuses with two polar nuclei to form the triploid endosperm (Lopes and Larkins, 1993). The seed coat differentiates after fertilization from maternally derived integuments. The embryo is surrounded by the endosperm, which, in turn, is enclosed within the maternal seed coat. Therefore, the size of a seed is determined by the coordinated growth of maternal sporophytic and zygotic tissues.

[0005] The size of seeds is influenced by a variety of cellular processes. Seed size is known to be influenced by parent-of-origin effects. The cross between a diploid female parent and tetraploid male parent produces larger F.sub.1 seeds, whereas the reciprocal cross generates smaller F.sub.1 seeds, suggesting that maternal or paternal excess of genome has a dramatic effect on seed size (Scott et al., 1998). Similar to interploidy crosses, crosses between wild type and met1 mutant with hypomethylated genomes show that larger F.sub.1 seeds are generated when the maternal parent is met1, while smaller F.sub.1 seeds are produced when the paternal parent is met1 (Xiao et al., 2006), suggesting that parent-of-origin effects may involve DNA methylation. In addition, the size of seeds is affected by the maternal and/or zygotic tissues. Several factors that influence seed size by the zygotic tissues have been recently identified in Arabidopsis. haiku (iku) and miniseed3 (mini3) mutants form small seeds due to the reduced growth and early cellularization of the endosperm (Garcia et al., 2003; Luo et al., 2005). IKU1, IKU2 and MINI3 function in the same pathway to promote endosperm growth in Arabidopsis (Garcia et al., 2003; Luo et al., 2005; Wang et al., 2010). SHORT HYPOCOTYL UNDER BLUE1 (SHB1) associates with both MINI3 and IKU2 promoters in vivo and may act with other proteins that bind to MINI3 and IKU2 promoters to promote endosperm growth in the early phase of seed development (Zhou et al., 2009). Seed size is also influenced by maternal tissues. Several factors that act in maternal tissues to influence seed size have been isolated. Arabidopsis TRANSPARENT TESTA GLABRA 2 (TTG2) promotes seed growth by increasing cell expansion in the integuments (Garcia et al., 2005; Ohto et al., 2009). APETALA2 (AP2) may restrict seed growth by limiting cell expansion in the integuments (Jofuku et al., 2005; Ohto et al., 2005; Ohto et al., 2009). By contrast, AUXIN RESPONSE FACTOR 2 (ARF2) and the predicted ubiquitin receptor CYP78A61 limit seed size by restricting cell proliferation in the integuments (Schruff et al., 2006; Li et al., 2008). However, CYP78A5/KLU promotes seed growth by increasing cell proliferation in the integuments of ovules (Adamski et al., 2009). Therefore, the integument or seed coat plays a key role in maternal control of seed size. In addition, many quantitative trait loci (QTLs) for seed size have been mapped in Arabidopsis and crops (Alonso-Blanco et al., 1999; Li et al., 2004; Fan et al., 2006; Song et al., 2007; Shomura et al., 2008; Weng et al., 2008). Three grain size QTLs have been recently cloned in rice, including GS3, GW2 and qSW5/GW5 (Fan et al., 2006; Song et al., 2007; Shomura et al., 2008; Weng et al., 2008). However, it is not clear whether these three factors act in maternal and/or zygotic tissues in rice.

[0006] Despite the importance of seed size, relatively little is known about the genetic and molecular mechanisms that control seed size.

[0007] Identification of factors that control the final size of seeds will not only advance understanding of the mechanisms of size control in plants, but may also have substantial practical applications for example in improving crop yield.

SUMMARY OF INVENTION

[0008] The present inventors have identified and characterized a genetic factor which alters seed size in plants. This may be useful, for example, in modulating seed size and improving yields in crop plants.

[0009] An aspect of the invention provides a method of modulating seed size in a plant which comprises;

[0010] altering the expression of a CYP78A polypeptide within cells of said plant.

[0011] Another aspect of the invention provides a method of producing a plant with an altered seed size comprising:

[0012] incorporating a heterologous nucleic acid which alters the expression of a CYP78A polypeptide into a plant cell by means of transformation, and;

[0013] regenerating the plant from one or more transformed cells.

[0014] Other aspects of the invention relate to plant cells with altered expression of a CYP78A polypeptide relative to controls, for example plant cells produced by a method described above; plants comprising such cells, and the seeds and progeny of such plants.

[0015] The CYP78A polypeptide may be a CYP78A6 polypeptide.

[0016] The expression of two or more CYP78A polypeptides may be altered in the plant cells.

[0017] The expression of one or more other growth factors, such as DA or BB may additionally be altered in the plant cells.

BRIEF DESCRIPTION OF DRAWINGS

[0018] FIG. 1 shows the isolation of an enhancer of da1-1 (eod3-1D). FIG. 1(A) shows seeds from wild-type, da1-1 and eod3-1D da1-1 plants (from left to right). FIG. 1(B) shows mature embryos of wild type, da1-1 and eod3-1D da1-1 (from left to right). FIG. 1(C) shows 10-d-old-seedlings of wild type, da1-1 and eod3-1D da1-1 (from left to right). FIG. 1(D) shows projective area of wild-type, da1-1 and eod3-1D da1-1 seeds. FIG. 1(E) shows seed weight of wild type, da1-1 and eod3-1D da1-1. FIG. 1(F) shows cotyledon area of 10-d-old wild-type, da1-1 and eod3-1D da1-1 seedlings. Values (D-F) are given as mean.+-.SE relative to the respective wild-type values, set at 100%. Bars: A, B, 0.5 mm; C, 5 mm.

[0019] FIG. 2 shows seed and organ size in the eod3-1D mutant. 2(A-D) show seeds (A), flowers (B), stamens and carpels (C), and siliques (D) of wild type (left) and eod3-1D (right). 2(E) shows projective area of wild-type and eod3-1D seeds. 2(F) shows seed weight of wild type and eod3-1D. 2(G) shows projective area of Col-0.times.Col-0 F.sub.1, CS4002.times.Col-0 F.sub.1 and eod3-1D.times.eod3-1D F.sub.1 seeds. Values (E-G) are given as mean.+-.SE relative to the respective wild-type values, set at 100%. Bars: A, B, C, D, 1 mm.

[0020] FIG. 3 shows the cloning of the EOD3 gene. 3(A) shows the structure of T-DNA insertion in the eod3-1D mutant. 3(B) shows expression levels of At2g46660 (EOD3) and At2g46670 in da1-1 and eod3-1D da1-1 seedlings. 3(C) shows projective area of wild-type, 35S::EOD3#7, 35S::EOD3#9 and eod3-1D seeds. 3(D) shows expression levels of EOD3 in wild-type, 35S::EOD3#7, 35S::EOD3#9 and eod3-1D seedlings. 3(E) shows phylogenetic tree of the CYP78A family members in Arabidopsis thaliana. Values (C and D) are given as mean.+-.SE relative to the wild-type value, set at 100%. **, P<0.01 compared with the wild type (Student's t-test).

[0021] FIG. 4 shows that EOD3 acts redundantly with CYP78A9 to influence seed size. 4(A) shows EOD3 gene structure. The start codon (ATG) and the stop codon (TAA) are indicated. Closed boxes indicate the coding sequence, and the line between boxes indicates intron. T-DNA insertion sites (eod3-ko1 and eod3-ko2) in the EOD3 gene were shown. 4(B) shows CYP78A9 gene structure. The start codon (ATG) and the stop codon (TAA) are indicated. Closed boxes indicate the coding sequence, and the line between boxes indicates intron. The T-DNA insertion site (cyp78a9-ko1) in the CYP78A9 gene was shown. 4(C) shows seeds from wild-type, eod3-ko1 cyp78a9-ko1 and eod3-ko2 cyp78a9-ko1 plants (from top to bottom). 4(D) Siliques from wild-type, eod3-ko1, eod3-ko2, cyp78a9-ko1, eod3-ko1 cyp78a9-ko1 and eod3-ko2 cyp78a9-ko1 plants (from left to right). 4(E) shows opened siliques from wild-type and eod3-ko1 cyp78a9-ko1 plants (from left to right). 4(F) shows projective area of wild-type, eod3-ko1, eod3-ko2, cyp78a9-ko1, eod3-ko1 cyp78a9-ko1 and eod3-ko2 cyp78a9-ko1 seeds. 4(G) shows seed weight of wild type, eod3-ko1, eod3-ko2, cyp78a9-ko1, eod3-ko1 cyp78a9-ko1 and eod3-ko2 cyp78a9-ko1. 4(H) shows silique length of wild type, eod3-ko1, eod3-ko2, cyp78a9-ko1, eod3-ko1 cyp78a9-ko1 and eod3-ko2 cyp78a9-ko1. 4(I and J) show seed length (I) and seed width (J) of wild type, eod3-ko1, cyp78a9-ko1 and eod3-ko1 cyp78a9-ko1. Values (F-J) are given as mean.+-.SE relative to the respective wild-type values, set at 100%. **, P<0.01 and *, P<0.05 compared with the wild type (Student's t-test). Bars: C, D, E, 1 mm.

[0022] FIG. 5 shows EOD3 acts maternally to control seed size. 5(A) shows projective area of Col-0.times.Col-0 F.sub.1, Col-0.times.eod3-ko1 cyp78a9-ko1(d) F eod3-ko1 cyp78a9-ko1(d).times.eod3-ko1 cyp78a9-ko1(d) F.sub.1 and eod3-ko1 cyp78a9-ko1(d).times.Col-0 F.sub.1 seeds. 5(B) shows projective area of Col-0.times.Col-0 F.sub.1, Col-0.times.eod3-1D F.sub.1, eod3-1D/+.times.eod3-1D/+(e/e) F.sub.1, eod3-1D/+.times.Col-0 (e/c) F.sub.1 seeds. Values (A and B) are given as mean.+-.SE relative to the respective wild-type values, set at 100%.

[0023] FIG. 6 shows cell size and cell number in the integuments of wild-type, eod3-ko1 cyp78a9-ko1 and eod3-1D developing seeds. 6(A) shows mature ovule perimeter. 6(B) shows the outer integument length at specific times after pollination, as measured from the insertion point at the funiculus to the tip at the micropyle. 6(C) shows the number of cells in the outer integument at 6DAP. 6(D) shows the average length of cells in the outer integument at 6DAP calculated from the outer integument length and cell number for individual seeds. Values (A-D) are given as mean.+-.SE. **, P<0.01 compared with the wild type (Student's t-test).

[0024] FIG. 7 shows cell size and cell number in cotyledons of mature wild-type, eod3-ko1 cyp78a9-ko1 and eod3-1D embryos. 7(A) shows mature embryos of wild type, eod3-ko1 cyp78a9-ko1 and eod3-1D. 7(B) shows cotyledon area of wild-type, eod3-ko1 cyp78a9-ko1 and eod3-1D embryos. 7(C) Average area of palisade cells in cotyledons of wild-type, eod3-ko1 cyp78a9-ko1 and eod3-1D embryos. Values (B and C) are given as mean.+-.SE relative to the respective wild-type values, set at 100%. **, P<0.01 compared with the wild type (Student's t-test). Bar: A, 0.25 mm.

[0025] FIG. 8 shows the expression pattern of EOD3. 8(A) shows RT-PCR analysis of the EOD3 gene expression. Total RNA was isolated from stems, roots, 10-d-old seedlings, leaves and inflorescences. 8(B-L) show EOD3 expression activity monitored by pEOD3::GUS transgene expression. Three GUS-expressing lines were observed and all showed a similar pattern, although they differed slightly in the intensity of the staining. Histochemical analysis of GUS activity in a 14-d-old seedling (B), a sepal (C), a petal (D), a stamen (E), a carpel (F), an inflorescence (G), the valve of a silique (H) and embryos (I-L). No GUS activity was detected in developing seeds. 8(M and N) show results of in situ hybridization with EOD3 antisense probe. Cross-section of the carpel of a stage 8 flower (M). Cross-section of the carpel of a stage 12 flower (N). The blue arrow indicates the central region of the septum, and the red arrow shows the funiculus. Bars: B, 2 mm; G, 1 mm; C, E, F, I, J, K, L, 100 .mu.m; D, M, N, 50 .mu.m. H, 200 .mu.m.

[0026] FIG. 9 shows genetic interactions of eod3 with da1-1 and ttg2-3 mutants. 9(A) shows the projective area of wild-type, eod3-1D, da1-1 and da1-1 eod3-1D seeds. 9(B) shows projective area of wild-type, ttg2-3, eod3-ko1 and ttg2-3 eod3-ko1 seeds. Values (A-B) are given as mean.+-.SE relative to the respective wild-type values, set at 100%.

[0027] FIG. 10 shows eod3-1D enhances the organ size phenotype of da1-1. 10A shows flowers of wild type, da1-1 and eod3-1D da1-1. 10(b) shows area of the fifth leaves in wild type, da1-1 and eod3-1D da1-1. Values (B) are given as mean.+-.SE relative to the respective wild-type values, set at 100%. Bar: A, 1 mm.

[0028] FIG. 11 shows organ size and reproductive development in eod3-1D. 11(A) shows sepal length (SL), petal length (PL), petal width (PW), petal area (PA), stamen length (STL) and carpel length (CL) of wild-type (left) and eod3-1D (right) flowers. 11(B and C) show the earliest-arising flowers on the primary inflorescences of wild type (B) and eod3-1D (C). 11(D and E) show pollens from the earliest-arising flowers were stained by using Alexander staining buffer. Values (A) are given as mean.+-.SE relative to the respective wild-type values, set at 100%. Bars: B, C, 1 mm; D,E, 100 .mu.m.

[0029] FIG. 12 shows phenotypes of wild-type, eod3-1D and 35S::EOD3 plants. 12A shows 56-d-old wild-type, eod3-1D, 35S::EOD3#7 and 35S::EOD3#9 plants. 12B shows primary inflorescence stems of wild-type, eod3-1D, 35S::EOD3#7 and 35S::EOD3#9 plants. 12C shows percent of fertile siliques on the primary inflorescence. Values (C) are given as mean.+-.SE. Bars: A, B, 1 cm.

[0030] FIG. 13 shows identification of eod3-ko1, eod3-ko2 and cyp78a9-ko1 mutants. 13(A-C) show PCR identification of T-DNA insertions in eod3-ko1 (A), eod3-ko2 (B), cyp78a9-ko1 (C) mutants with T-DNA specific primers and flanking primers.

[0031] FIG. 14 shows EOD3 acts maternally to influence seed size. Projective area of individual seeds from eod3-1D/+ plants fertilized with wild-type pollen was measured. These seeds were further genotyped for the eod3-1D mutation. The data shows that the eod3-1D mutation is not associated with variation in the size of these seeds (P>0.05, Student's t-test).

[0032] FIG. 15 shows mature ovules from Col-0, eod3-ko1 cyp78a9-ko1 and eod3-1D plants. Bars: A, B, C, 50 .mu.m.

[0033] FIG. 16 shows EOD3 and CYP78A9 expression in developing seeds. 16(A and B) show results of in situ hybridization with EOD3 antisense probe. 16(C and D) show results of in situ hybridization with EOD3 sense probe. 16(E and F) show results of in situ hybridization with CYP78A9 antisense probe. 16(G and H) show results of in situ hybridization with CYP78A9 sense probe. Bars: A, B, C, D, E, F, G, H, 50 .mu.m.

[0034] FIG. 17 shows expression of EOD3 in developing seeds using RT-PCR analysis of the EOD3 gene expression. Total RNA was isolated from developing seeds within elongated siliques.

[0035] FIG. 18 shows phylogenic analysis of Arabidopsis CYP78A6 and its orthologues.

[0036] FIG. 19 shows the Gateway Binary Vector pIPKb002 containing the Zm-ubiqutin promoter used to expresss EOD3 in Oryza sativa.

[0037] FIG. 20 shows seed size in TO transgenic Oryza sativa overexpressing EOD3. * represents p<0.01 in t-test with the smallest seed line (11-5).

[0038] Table 1 shows phenotypes of wild-type, eod3-ko1, cyp78a9-ko1, eod3-ko1 cyp78a9-ko1 and eod3-1D plants.

[0039] Table 2 shows developmental stages of embryogenesis.

[0040] Table 3 shows primers.

DETAILED DESCRIPTION OF EMBODIMENTS OF THE INVENTION

[0041] In various aspects, the invention provides methods which relate to the modulation of CYP78A expression in plant cells. This modulation may be useful in altering, for example increasing or decreasing, seed size in plants.

[0042] CYP78A polypeptides are a sub-family of cytochrome p450 (CYP) dependent monooxygenases which are found only in plants. CYP78A polypeptides may be defined by phylogenetic analysis on the basis of overall identity and sequence conservation within domains (Chapple Annu. Rev. Plant Physiol. Plant Mol. Biol. (1998) 49:311-43)

[0043] A CYP78A polypeptide may comprise an amino acid sequence which is shown in FIG. 18 or an amino acid sequence which is selected from the group consisting of SEQ ID NOS:

[0044] 42 to 83 or an amino acid sequence which is a variant or fragment of one of these sequences which retains CYP78A activity.

[0045] Other CYP78A polypeptide sequences which include the characteristic features set out above may be identified using standard sequence analysis tools.

[0046] In some preferred embodiments, the CYP78A polypeptide may be a member of the phylogenetic grouping of CYP78A polypeptides which comprises CYP78A6, CYP78A8 and CYP78A9 and excludes CYP78A7, CYP78A5 and CYP78A10, as shown in FIG. 3E (i.e. a CYP78A6-clade polypeptide).

[0047] For example, a CYP78A6-clade polypeptide may comprise 1, 2, 3, 4, 5, 6 or all 7 of the amino acid sequence motifs of SEQ ID NOS: 84 to 90;

[0048] GGAWGKYX.sub.1R (SEQ ID NO: 84), wherein X.sub.1 is any amino acid, preferably G, H or T, most preferably G.

[0049] X.sub.2G X.sub.3GVGSMSX.sub.4 X.sub.5S X.sub.6X.sub.7AHR (SEQ ID NO: 85), wherein X.sub.2 is any amino acid, preferably V or N, most preferably V; wherein X.sub.3 is any amino acid, preferably K or R, most preferably K; wherein X.sub.4 is absent or any amino acid, preferably absent or R, most preferably absent; wherein X.sub.5 is any amino acid, preferably M or S, most preferably M; wherein X.sub.6 is any amino acid, preferably S, N or H, most preferably S; and wherein X.sub.7 is any amino acid, preferably T or V, most preferably T.

[0050] MASGX.sub.8X.sub.9X.sub.10X.sub.11VVTCX.sub.12X.sub.13VAKNX.sub.14S- VADRV (SEQ ID NO: 86), wherein X.sub.8 is any amino acid, preferably T or D, most preferably T; wherein X.sub.9 is any amino acid, preferably T or R, most preferably R; wherein X.sub.10 is absent or any amino acid, preferably absent or K, most preferably absent; wherein X.sub.11 is absent or any amino acid, preferably absent or V, most preferably absent; wherein X.sub.12 is any amino acid, preferably N or H, most preferably N; wherein X.sub.13 is any amino acid, preferably D or A, most preferably D; wherein X.sub.14 is absent or any amino acid, preferably absent or S, most preferably absent.

TABLE-US-00001 (SEQ ID NO: 87) VGYDGTNWTDHW (SEQ ID NO: 88) AVWMRGTDVA (SEQ ID NO: 89) KVRHGSWARX.sub.15TDT,

wherein X.sub.15 is any amino acid, preferably A or S, most preferably A.

[0051] VAGTTAMVNMWAX.sub.16X.sub.17X.sub.18DHVWX.sub.19X.sub.20KRVAKGX.sub- .21SVGSDRAGSGX.sub.22RX.sub.23CG KNGTTV (SEQ ID NO: 90); wherein independently, X.sub.16 is any amino acid, preferably A or V, most preferably V; X.sub.17 is absent or any amino acid, preferably absent or S, most preferably S; X.sub.18 is any amino acid, preferably H or R, most preferably H; X.sub.19 is any amino acid, preferably V, N or D, most preferably V; X.sub.20 is absent or any amino acid, preferably absent or D, most preferably D; X.sub.21 is any amino acid, preferably A or V, most preferably V; X.sub.22 is any amino acid, preferably R or K, most preferably R; and X.sub.23 is absent or any amino acid, preferably absent or V, most preferably absent.

[0052] For example, a CYP78A6-clade polypeptide may comprise SEQ NO:84 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 85 to 90; a CYP78A6-clade polypeptide may comprise SEQ NO:85 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 84 and 86 to 90; a CYP78A6-clade polypeptide may comprise SEQ NO:86 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 84, 85 and 87 to 90; a CYP78A6-clade polypeptide may comprise SEQ NO:87 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 84 to 86 and 88 to 90; a CYP78A6-clade polypeptide may comprise SEQ NO:88 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 84 to 87, 89 or 90; a CYP78A6-clade polypeptide may comprise SEQ NO:89 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 84 to 88 and 90; or a CYP78A6-clade polypeptide may comprise SEQ NO:90 in combination with any 1, 2, 3, 4, 5 or all 6 of SEQ ID NOS: 84 to 89.

[0053] In some preferred embodiments, a CYP78A6-clade polypeptide may comprise an amino acid sequence selected from the group consisting of SEQ ID NOS:42 to 44, or may be variant or a fragment of one of these sequences which retains CYP78A activity.

[0054] Preferably, the CYP78A6-clade polypeptide is a CYP78A6 polypeptide or a functional homologue thereof, as described herein. A CYP78A6 polypeptide may comprise 1, 2, 3, 4, 5, 6 or all 7 of the amino acid sequence motifs of SEQ ID NOS: 87, 88 and 91 to 95;

TABLE-US-00002 (SEQ ID NO: 91) GGAWGKYGRSGSYKTGN (SEQ ID NO: 92) VGKGVGSMSMSSTAHR (SEQ ID NO: 93) MASGTRVVTCNDVAKNSVADRV (SEQ ID NO: 94) KVRHGSWARATDT (SEQ ID NO: 95) VAGTTAMVNMWAVSHDHVWVDKRVAKGVSVGSDRAGSGRRCGKNGTTV.

[0055] For example, a CYP78A6 polypeptide may comprise the amino acid sequence of A. thaliana CYP78A6 (At2g46660) (SEQ ID NO: 42) or may be a fragment or variant of this sequence which retains CYP78A activity. Other CYP78A6 polypeptides may comprise the amino acid sequence of any one of SEQ ID NOS: 42 to 83 or may be a fragment or variant of the sequence which retains CYP78A activity.

[0056] A CYP78A polypeptide which is a variant of a reference CYP78A sequence, such as any one of SEQ ID NOS: 42 to 83, preferably SEQ ID NOS: 42 to 44, most preferably SEQ ID NO:42, may comprise an amino acid sequence having at least 20%, at least 30%, at least 40%, at least 50%, at least 55%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 98% sequence identity to the reference sequence.

[0057] Particular amino acid sequence variants may differ from the reference CYP78A sequence, such as any one of SEQ ID NOS: 42 to 83, by insertion, addition, substitution or deletion of 1 amino acid, 2, 3, 4, 5-10, 10-20 20-30, 30-50, or more than 50 amino acids.

[0058] Sequence similarity and identity are commonly defined with reference to the algorithm GAP (Wisconsin Package, Accelerys, San Diego USA). GAP uses the Needleman and Wunsch algorithm to align two complete sequences that maximizes the number of matches and minimizes the number of gaps. Generally, default parameters are used, with a gap creation penalty=12 and gap extension penalty=4.

[0059] Use of GAP may be preferred but other algorithms may be used, e.g. BLAST (which uses the method of Altschul et al. (1990) J Mol. Biol. 215: 405-410), FASTA (which uses the method of Pearson and Lipman (1988) PNAS USA 85: 2444-2448), or the Smith-Waterman algorithm (Smith and Waterman (1981) J Mol Biol. 147: 195-197), or the TBLASTN program, of Altschul et al. (1990) supra, generally employing default parameters. In particular, the psi-Blast algorithm (Nucl. Acids Res. (1997) 25 3389-3402) may be used.

[0060] Sequence comparison may be made over the full-length of the relevant sequence described herein.

[0061] A CYP78A polypeptide which is a fragment of a reference CYP78A sequence, such as any one of SEQ ID NOS: 42 to 83, may consist of fewer amino acid residues than the full-length sequence. A CYP78A polypeptide fragment retains CYP78A activity and may, for example, comprise 100 or more, 150 or more, 200 or more or 250 or more amino acids.

[0062] Methods of increasing seed size in plants as described herein may comprise increasing expression of a CYP78A polypeptide in one or more cells of the plant relative to controls.

[0063] CYP78A expression may be increased by mutation. For example, a nucleic acid sequence which represses expression of a CYP78A coding sequence may be mutated. Suitable mutation methods, such as insertional activation using a heterologous nucleic acid, are well known in the art.

[0064] Alternatively, CYP78A expression may be increased by over-expression of a CYP78A coding sequence. For example, a heterologous nucleic acid encoding the CYP78A polypeptide may be expressed within the cells of a plant or a heterologous nucleic acid which promotes or increases expression of an endogenous CYP78A coding sequence may be inserted into the cells of a plant.

[0065] In some preferred embodiments, a nucleic acid encoding a CYP78A polypeptide may comprise the nucleotide sequence of SEQ ID NO: 1 or any one of SEQ ID NOS: 2 to 41 or may be a variant or fragment of this sequence which encodes a polypeptide which retains CYP78A activity.

[0066] Other nucleic acid sequences which encode CYP78A polypeptides are available on public databases.

[0067] A variant sequence may be a mutant, homologue, or allele of a reference CYP78A nucleotide sequence, such as any one of SEQ ID NOS: 1 to 41, or a reference BB sequence, such as SEQ ID NO: 96 and may differ from the reference CYP78A or BB sequence by one or more of addition, insertion, deletion or substitution of one or more nucleotides in the nucleic acid, leading to the addition, insertion, deletion or substitution of one or more amino acids in the encoded polypeptide. Of course, changes to the nucleic acid that make no difference to the encoded amino acid sequence are included. A nucleic acid encoding a CYP78A polypeptide may comprise a sequence having at least 20% or at least 30% sequence identity with the reference CYP78A nucleic acid sequence, preferably at least 40%, at least 50%, at least 60%, at least 65%, at least 70%, at least 80%, at least 90%, at least 95% or at least 98%. A nucleic acid encoding a BB polypeptide may comprise a sequence having at least 20% or at least 30% sequence identity with the reference BB nucleic acid sequence, preferably at least 40%, at least 50%, at least 60%, at least 65%, at least 70%, at least 80%, at least 90%, at least 95% or at least 98%. Sequence identity is described above.

[0068] A fragment or variant may comprise a sequence which encodes a functional CYP78A polypeptide i.e. a polypeptide which retains one or more functional characteristics of the polypeptide encoded by the wild-type CYP78A gene, for example, cytochrome p450 monooxygenase activity.

[0069] A nucleic acid comprising a nucleotide sequence which is a variant of a reference CYP78A6 nucleic acid sequence, such as any one of SEQ ID NOS: 1 to 41, may selectively hybridise under stringent conditions with this nucleic acid sequence or the complement thereof.

[0070] Stringent conditions include, e.g. for hybridization of sequences that are about 80 to 90% identical, hybridization overnight at 42.degree. C. in 0.25M Na.sub.2HPO.sub.4, pH 7.2, 6.5% SDS, 10% dextran sulfate and a final wash at 55.degree. C. in 0.1.times.SSC, 0.1% SDS. For detection of sequences that are greater than about 90% identical, suitable conditions include hybridization overnight at 65.degree. C. in 0.25M Na.sub.2HPO.sub.4, pH 7.2, 6.5% SDS, 10% dextran sulfate and a final wash at 60.degree. C. in 0.1.times.SSC, 0.1% SDS.

[0071] An alternative, which may be particularly appropriate with plant nucleic acid preparations, is a solution of 5.times.SSPE (final 0.9 M NaCl, 0.05M sodium phosphate, 0.005M EDTA pH 7.7), 5.times.Denhardt's solution, 0.5% SDS, at 50.degree. C. or 65.degree. C. overnight. Washes may be performed in 0.2.times.SSC/0.1% SDS at 65.degree. C. or at 50-60.degree. C. in 1.times.SSC/0.1% SDS, as required.

[0072] Nucleic acids as described herein may be wholly or partially synthetic. In particular, they may be recombinant in that nucleic acid sequences which are not found together in nature (do not run contiguously) have been ligated or otherwise combined artificially. Alternatively, they may have been synthesised directly e.g. using an automated synthesiser.

[0073] The nucleic acid may of course be double- or single-stranded, cDNA or genomic DNA, or RNA. The nucleic acid may be wholly or partially synthetic, depending on design. Naturally, the skilled person will understand that where the nucleic acid includes RNA, reference to the sequence shown should be construed as reference to the RNA equivalent, with U substituted for T.

[0074] Nucleic acid encoding a CYP78A polypeptide may be expressed in the same plant species or variety from which it was originally isolated or in a different plant species or variety (i.e. a heterologous plant).

[0075] "Heterologous" indicates that the gene/sequence of nucleotides in question or a sequence regulating the gene/sequence in question, has been introduced into said cells of the plant or an ancestor thereof, using genetic engineering or recombinant means, i.e. by human intervention. Nucleotide sequences which are heterologous to a plant cell may be non-naturally occurring in cells of that type, variety or species (i.e. exogenous or foreign) or may be sequences which are non-naturally occurring in that sub-cellular or genomic environment of the cells or may be sequences which are non-naturally regulated in the cells i.e. operably linked to a non-natural regulatory element. "Isolated" indicates that the isolated molecule (e.g. polypeptide or nucleic acid) exists in an environment which is distinct from the environment in which it occurs in nature. For example, an isolated nucleic acid may be substantially isolated with respect to the genomic environment in which it naturally occurs. An isolated nucleic acid may exist in an environment other than the environment in which it occurs in nature.

[0076] Methods of reducing seed size in plants as described herein may comprise reducing or abolishing expression of a CYP78A polypeptide in one or more cells of the plant relative to controls.

[0077] CYP78A expression may be reduced or abolished by mutation. For example, nucleic acid sequence encoding a CYP78A polypeptide within cells of said plant may be mutated, for example by insertion of a heterologous nucleic acid, within the plant cells. Alternatively, nucleic acid which regulates the expression of a CYP78A coding sequence within cells of said plant, such as a promoter or enhancer sequence, may be mutated, for example by insertion of a heterologous nucleic acid, within the plant cells.

[0078] The expression of CYP78A polypeptide may be reduced or abolished by mutating the nucleic acid sequences in the plant cell which encode the active protein and regenerating a plant from the mutated cell. The nucleic acids may be mutated by insertion or deletion of one or more nucleotides. Techniques for the inactivation or knockout of target genes are well-known in the art.

[0079] CYP78A expression may be reduced or abolished by suppression. For example, a heterologous nucleic encoding a suppressor nucleic acid which suppresses expression of a CYP78A polypeptide may be expressed within the plant cells.

[0080] The suppression of the expression of target polypeptides in plant cells is well-known in the art. Suitable suppressor nucleic acids may be copies of all or part of the target CYP78A gene inserted in antisense or sense orientation or both relative to the CYP78A gene, to achieve reduction in expression of the CYP78A gene. See, for example, van der Krol et al., (1990) The Plant Cell 2, 291-299; Napoli et al., (1990) The Plant Cell 2, 279-289; Zhang et al., (1992) The Plant Cell 4, 1575-1588, and U.S. Pat. No. 5,231,020. Further refinements of this approach may be found in WO95/34668 (Biosource); Angell & Baulcombe (1997) The EMBO Journal 16, 12:3675-3684; and Voinnet & Baulcombe (1997) Nature 389: pg 553.

[0081] In some embodiments, the suppressor nucleic acids may be sense suppressors of expression of active CYP78A protein.

[0082] A suitable sense suppressor nucleic acid may be a double stranded RNA (Fire A. et al Nature, Vol 391, (1998)). dsRNA mediated silencing is gene specific and is often termed RNA interference (RNAi). RNAi is a two step process. First, dsRNA is cleaved within the cell to yield short interfering RNAs (siRNAs) of about 21-23 nt length with 5' terminal phosphate and 3' short overhangs (.about.2 nt). The siRNAs target the corresponding mRNA sequence specifically for destruction (Zamore P. D. Nature Structural Biology, 8, 9, 746-750, (2001)

[0083] siRNAs (sometimes called microRNAs) down-regulate gene expression by binding to complementary RNAs and either triggering mRNA elimination (RNAi) or arresting mRNA translation into protein. siRNA may be derived by processing of long double stranded RNAs and when found in nature are typically of exogenous origin. Micro-interfering RNAs (miRNA) are endogenously encoded small non-coding RNAs, derived by processing of short hairpins. Both siRNA and miRNA can inhibit the translation of mRNAs bearing partially complementary target sequences without RNA cleavage and degrade mRNAs bearing fully complementary sequences.

[0084] Accordingly, the present invention provides the use of RNAi sequences based on the CYP78A nucleic acid sequence for suppression of the expression of the CYP78A polypeptide. For example, an RNAi sequence may correspond to a fragment of any one of SEQ ID NOS: 1 to 41 or other CYP78A nucleic acid sequence referred to above, or a variant thereof.

[0085] siRNA molecules are typically double stranded and, in order to optimise the effectiveness of RNA mediated down-regulation of the function of a target gene, it is preferred that the length and sequence of the siRNA molecule is chosen to ensure correct recognition of the siRNA by the RISC complex that mediates the recognition by the siRNA of the mRNA target and so that the siRNA is short enough to reduce a host response.

[0086] miRNA ligands are typically single stranded and have regions that are partially complementary enabling the ligands to form a hairpin. miRNAs are RNA sequences which are transcribed from DNA, but are not translated into protein. A DNA sequence that codes for a miRNA is longer than the miRNA. This DNA sequence includes the miRNA sequence and an approximate reverse complement. When this DNA sequence is transcribed into a single-stranded RNA molecule, the miRNA sequence and its reverse-complement base pair to form a partially double stranded RNA segment. The design of microRNA sequences is discussed on John et al, PLoS Biology, 11(2), 1862-1879, 2004.

[0087] Typically, the RNA molecules intended to mimic the effects of siRNA or miRNA have between 10 and 40 ribonucleotides (or synthetic analogues thereof), more preferably between 17 and 30 ribonucleotides, more preferably between 19 and 25 ribonucleotides and most preferably between 21 and 23 ribonucleotides. In some embodiments of the invention employing double-stranded siRNA, the molecule may have symmetric 3' overhangs, e.g. of one or two (ribo)nucleotides, typically a UU of dTdT 3' overhang. Based on the disclosure provided herein, the skilled person can readily design suitable siRNA and miRNA sequences, for example using one of the numerous publically available on-line siRNA finders, such as GenScript siRNA Target Finder, GenScript USA Inc. siRNA and miRNA sequences can be synthetically produced and added exogenously to cause gene downregulation or produced using expression systems (e.g. vectors). In a preferred embodiment, the siRNA is synthesized synthetically.

[0088] Longer double stranded RNAs may be processed in the cell to produce siRNAs (see for example Myers (2003) Nature Biotechnology 21:324-328). The longer dsRNA molecule may have symmetric 3' or 5' overhangs, e.g. of one or two (ribo) nucleotides, or may have blunt ends. The longer dsRNA molecules may be 25 nucleotides or longer. Preferably, the longer dsRNA molecules are between 25 and 30 nucleotides long. More preferably, the longer dsRNA molecules are between 25 and 27 nucleotides long. Most preferably, the longer dsRNA molecules are 27 nucleotides in length. dsRNAs 30 nucleotides or more in length may be expressed using the vector pDECAP (Shinagawa et al., Genes and Dev., 17, 1340-5, 2003).

[0089] Another alternative is the expression of a short hairpin RNA molecule (shRNA) in the cell. shRNAs are more stable than synthetic siRNAs. A shRNA consists of short inverted repeats separated by a small loop sequence. One inverted repeat is complementary to the gene target. In the cell the shRNA is processed by DICER into a siRNA which degrades the target gene mRNA and suppresses expression. In a preferred embodiment the shRNA is produced endogenously (within a cell) by transcription from a vector. shRNAs may be produced within a cell by transfecting the cell with a vector encoding the shRNA sequence under control of a RNA polymerase III promoter such as the human H1 or 7SK promoter or a RNA polymerase II promoter. Alternatively, the shRNA may be synthesised exogenously (in vitro) by transcription from a vector. The shRNA may then be introduced directly into the cell. Preferably, the shRNA molecule comprises a partial sequence of SHR. For example, the shRNA sequence is between 40 and 100 bases in length, more preferably between 40 and 70 bases in length. The stem of the hairpin is preferably between 19 and 30 base pairs in length. The stem may contain G-U pairings to stabilise the hairpin structure.

[0090] siRNA molecules, longer dsRNA molecules or miRNA molecules may be made recombinantly by transcription of a nucleic acid sequence, preferably contained within a vector. Preferably, the siRNA molecule, longer dsRNA molecule or miRNA molecule comprises a partial sequence of any one of SEQ ID NOS: 1 to 41 or a variant thereof, preferably any one of SEQ ID NOS: 1, 2, or 3 or a variant thereof, most preferably SEQ ID NO: 1 or a variant thereof.

[0091] In other embodiments, the suppressor nucleic acid may be an anti-sense suppressor of expression of a CYP78A6 polypeptide. In using anti-sense sequences to down-regulate gene expression, a nucleotide sequence is placed under the control of a promoter in a "reverse orientation" such that transcription yields RNA which is complementary to normal mRNA transcribed from the "sense" strand of the target gene. See, for example, Rothstein et al, 1987; Smith et al, (1988) Nature 334, 724-726; Zhang et al, (1992) The Plant Cell 4, 1575-1588, English et al., (1996) The Plant Cell 8, 179-188. Antisense technology is also reviewed in Bourque, (1995), Plant Science 105, 125-149, and Flavell (1994) PNAS USA 91, 3490-3496.

[0092] An anti-sense suppressor nucleic acid may comprise an anti-sense sequence of at least 10 nucleotides from a nucleotide sequence is a fragment of any one of SEQ ID NOS: 1 to 41 or other CYP78A sequence referred to above, or a variant thereof.

[0093] It may be preferable that there is complete sequence identity in the sequence used for down-regulation of expression of a target sequence, and the target sequence, although total complementarity or similarity of sequence is not essential. One or more nucleotides may differ in the sequence used from the target gene. Thus, a sequence employed in a down-regulation of gene expression in accordance with the present invention may be a wild-type sequence (e.g. gene) selected from those available, or a variant of such a sequence.

[0094] The sequence need not include an open reading frame or specify an RNA that would be translatable. It may be preferred for there to be sufficient homology for the respective anti-sense and sense RNA molecules to hybridise. There may be down regulation of gene expression even where there is about 5%, 10%, 15% or 20% or more mismatch between the sequence used and the target gene. Effectively, the homology should be sufficient for the down-regulation of gene expression to take place.

[0095] A nucleic acid encoding a CYP78A polypeptide or a CYP78A suppressor as described herein may be operably linked to a heterologous regulatory sequence, such as a promoter, for example a constitutive, inducible, tissue-specific or developmental specific promoter.

[0096] Many suitable regulatory sequences are known in the art and may be used in accordance with the invention. Examples of suitable regulatory sequences may be derived from a plant virus, for example the Cauliflower Mosaic Virus 35S (CaMV 35S) gene promoter that is expressed at a high level in virtually all plant tissues (Benfey et al, (1990) EMBO J 9: 1677-1684). Other suitable constitutive regulatory elements include the cauliflower mosaic virus 19S promoter; the Figwort mosaic virus promoter; and the nopaline synthase (nos) gene promoter (Singer et al., Plant Mol. Biol. 14:433 (1990); An, Plant Physiol. 81:86 (1986)).

[0097] Constructs for expression of the CYP78A genes or CYP78A suppressors under the control of a strong constitutive promoter (the 35S promoter) are exemplified below but those skilled in the art will appreciate that a wide variety of other promoters may be employed to advantage in particular contexts.

[0098] A tissue-specific promoter may be employed to express the CYP78A polypeptide or CYP78A suppressor in a specific tissue or organ.

[0099] For example, a seed-, seed-coat- or integument-specific promoter may be used to express the CYP78A polypeptide or CYP78A suppressor in seeds. Suitable promoters include, for example Phaseolus vulgaris phas promoter, Vicia faba leB4-, usp- or sbp-promoters, Soybean .beta.-conglycinin .alpha.-subunit promoter, Brassica FAE1 promoter and At4g12960 promoter (AtGILTpro) (Wu et al Plant Cell Rep (2011) 30:75-80).

[0100] Alternatively, or in addition, one might select an inducible promoter. In this way, for example, the CYP78A polypeptide or suppressor may be expressed at specific times or places in order to obtain desired changes in organ growth. Inducible promoters include the alcohol inducible AlcA gene-expression system (Roslan et al., Plant Journal; 2001 Oct; 28(2):225-35) may be employed.

[0101] The nucleic acid encoding the CYP78A polypeptide or CYP78A suppressor may be contained on a nucleic acid construct or vector. The construct or vector is preferably suitable for transformation into and/or expression within a plant cell. A vector is, inter alia, any plasmid, cosmid, phage or Agrobacterium binary vector in double or single stranded linear or circular form, which may or may not be self transmissible or mobilizable, and which can transform prokaryotic or eukaryotic host, in particular a plant host, either by integration into the cellular genome or exist extrachromasomally (e.g. autonomous replicating plasmid with an origin of replication).

[0102] Specifically included are shuttle vectors by which is meant a DNA vehicle capable, naturally or by design, of replication in two different organisms, which may be selected from Actinomyces and related species, bacteria and eukaryotic (e.g. higher plant, mammalia, yeast or fungal) cells.

[0103] A construct or vector comprising nucleic acid as described above need not include a promoter or other regulatory sequence, particularly if the vector is to be used to introduce the nucleic acid into cells for recombination into the genome.

[0104] Constructs and vectors may further comprise selectable genetic markers consisting of genes that confer selectable phenotypes such as resistance to antibiotics such as kanamycin, hygromycin, phosphinotricin, chlorsulfuron, methotrexate, gentamycin, spectinomycin, imidazolinones, glyphosate and d-amino acids.

[0105] Those skilled in the art can construct vectors and design protocols for recombinant gene expression, for example in a microbial or plant cell. Suitable vectors can be chosen or constructed, containing appropriate regulatory sequences, including promoter sequences, terminator fragments, polyadenylation sequences, enhancer sequences, marker genes and other sequences as appropriate. For further details see, for example, Molecular Cloning: a Laboratory Manual: 3rd edition, Sambrook et al, 2001, Cold Spring Harbor Laboratory Press and Protocols in Molecular Biology, Second Edition, Ausubel et al. eds. John Wiley & Sons, 1992. Specific procedures and vectors previously used with wide success upon plants are described by Bevan, Nucl. Acids Res. (1984) 12, 8711-8721), and Guerineau and Mullineaux, (1993) Plant transformation and expression vectors. In: Plant Molecular Biology Labfax (Croy RRD ed) Oxford, BIOS Scientific Publishers, pp 121-148.

[0106] When introducing a chosen nucleic acid construct into a cell, certain considerations must be taken into account, well known to those skilled in the art. The nucleic acid to be inserted should be assembled within a construct that contains effective regulatory elements that will drive transcription. There must be available a method of transporting the construct into the cell. Once the construct is within the cell membrane, integration into the endogenous chromosomal material either will or will not occur. Finally, the target cell type is preferably such that cells can be regenerated into whole plants.

[0107] Those skilled in the art will also appreciate that in producing constructs for achieving expression of the genes according to this invention, it is desirable to use a construct and transformation method which enhances expression of the nucleic acid encoding the CYP78A polypeptide or CYP78A suppressor. Integration of a single copy of the gene into the genome of the plant cell may be beneficial to minimize gene silencing effects. Likewise, control of the complexity of integration may be beneficial in this regard. Of particular interest in this regard is transformation of plant cells utilizing a minimal gene expression construct according to, for example, EP1407000B1, herein incorporated by reference for this purpose.

[0108] Techniques well known to those skilled in the art may be used to introduce nucleic acid constructs and vectors into plant cells to produce transgenic plants with the properties described herein.

[0109] Agrobacterium transformation is one method widely used by those skilled in the art to transform plant species. Production of stable, fertile transgenic plants is now routine in the art (see for example Toriyama, et al. (1988) Bio/Technology 6, 1072-1074; Zhang, et al. (1988) Plant Cell Rep. 7, 379-384; Zhang, et al. (1988) Theor Appl Genet 76, 835-840; Shimamoto, et al. (1989) Nature 338, 274-276; CYP78A6tta, et al. (1990) Bio/Technology 8, 736-740; Christou, et al. (1991) Bio/Technology 9, 957-962; Peng, et al. (1991) International Rice Research Institute, Manila, Philippines 563-574; Cao, et al. (1992) Plant Cell Rep. 11, 585-591; Li, et al. (1993) Plant Cell Rep. 12, 250-255; Rathore, et al. (1993) Plant Molecular Biology 21, 871-884; Fromm, et al. (1990) Bio/Technology 8, 833-839; Gordon-Kamm, et al. (1990) Plant Cell 2, 603-618; D'Halluin, et al. (1992) Plant Cell 4, 1495-1505; Walters, et al. (1992) Plant Molecular Biology 18, 189-200; Koziel, et al. (1993) Biotechnology 11, 194-200; Vasil, I. K. (1994) Plant Molecular Biology 25, 925-937; Weeks, et al. (1993) Plant Physiology 102, 1077-1084; Somers, et al. (1992) Bio/Technology 10, 1589-1594; WO92/14828; Nilsson, O. et al (1992) Transgenic Research 1, 209-220).

[0110] Other methods, such as microprojectile or particle bombardment (U.S. Pat. No. 5,100,792, EP-A-444882, EP-A-434616), electroporation (EP 290395, WO 8706614), microinjection (WO 92/09696, WO 94/00583, EP 331083, EP 175966, Green et al. (1987) Plant Tissue and Cell Culture, Academic Press), direct DNA uptake (DE 4005152, WO 9012096, U.S. Pat. No. 4,684,611), liposome mediated DNA uptake (e.g. Freeman et al. Plant Cell Physiol. 29: 1353 (1984)) or the vortexing method (e.g. Kindle, PNAS U.S.A. 87: 1228 (1990d)) may be preferred where Agrobacterium transformation is inefficient or ineffective, for example in some gymnosperm species.

[0111] Physical methods for the transformation of plant cells are reviewed in Oard, 1991, Biotech. Adv. 9: 1-11.

[0112] Alternatively, a combination of different techniques may be employed to enhance the efficiency of the transformation process, e.g. bombardment with Agrobacterium coated microparticles (EP-A-486234) or microprojectile bombardment to induce wounding followed by co-cultivation with Agrobacterium (EP-A-486233).

[0113] Following transformation, a plant may be regenerated, e.g. from single cells, callus tissue or leaf discs, as is standard in the art. Almost any plant can be entirely regenerated from cells, tissues and organs of the plant. Available techniques are reviewed in Vasil et al., Cell Culture and Somatic Cell Genetics of Plants, Vol I, II and III, Laboratory Procedures and Their Applications, Academic Press, 1984, and Weissbach and Weissbach, Methods for Plant Molecular Biology, Academic Press, 1989.

[0114] The particular choice of a transformation technology will be determined by its efficiency to transform certain plant species as well as the experience and preference of the person practising the invention with a particular methodology of choice. It will be apparent to the skilled person that the particular choice of a transformation system to introduce nucleic acid into plant cells is not essential to or a limitation of the invention, nor is the choice of technique for plant regeneration.

[0115] In some embodiments, the plant cell may further comprise altered expression of a DA protein, such as DA-1, and/or EOD1/BB protein. For example, expression of a DA and/or EOD1/BB protein may be reduced or abolished in plant cells in which CYP78A expression is increased, or dominant negative forms of DA and/or EOD1/BB proteins may be expressed. Expression of a DA and/or EOD1/BB protein may be increased in plant cells in which CYP78A expression is reduced.

[0116] DA proteins possess a characteristic domain structure comprising

a LIM domain, a UIM1 domain and a UIM2 domain (Li et al Genes & Dev, 2008. 22: 1331-1336; WO2009/04752). ADA polypeptide may comprise the amino acid sequence of SEQ ID NO: 42 (AT1G19270; NP_173361.1 GI: 15221983) or may be a fragment or variant of this sequence which retains DA activity.

[0117] Big Brother (EOD1/BB) is an E3 ubiquitin ligase which is known to repress plant organ growth (Disch Curr Biol 16 272-279 (2006)). A BB protein may comprise the amino acid sequence of At3g63530 NP_001030922.1 GI: 79316205 (SEQ ID NO: 97), or may be a fragment or variant which retains BB activity or is capable of interfering with the function of BB.

[0118] A BB protein or DA protein which is a variant of a reference BB or DA sequence described above may comprise an amino acid sequence having at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 98% sequence identity to the reference sequence. Sequence identity is described in more detail above.

[0119] DA proteins and EOD1/BB proteins, which may include AtDA1 (At1G19270) and AtBB (At3g63530), respectively, and variants thereof, are described and defined in detail in WO2009/04752, which is incorporated herein by reference for all purposes.

[0120] Particular amino acid sequence variants may differ from the DA polypeptide of SEQ ID NO: 42 or the BB polypeptide of SEQ ID NO: 97 (At3g63530) by insertion, addition, substitution or deletion of 1 amino acid, 2, 3, 4, 5-10, 10-20 20-30, 30-50, or more than 50 amino acids.

[0121] Plant as described herein having altered CYP78A expression in one or more cells therein, for example abolished, reduced, or increased CYP78A expression relative to controls, may be sexually or asexually propagated or off-spring or descendants may be grown.

[0122] Another aspect of the invention provides a method of producing a plant with an altered seed size comprising:

[0123] incorporating a heterologous nucleic acid which alters the expression of a CYP78A polypeptide into a plant cell by means of transformation, and;

[0124] regenerating the plant from one or more transformed cells.

[0125] As described above, the heterologous nucleic acid may encode a CYP78A polypeptide or a CYP78A suppressor molecule or may inactivate the endogenous CYP78A coding sequence of the plant or a regulatory sequence thereof, for example a repressor or enhancer.

[0126] The altered phenotype of the plant produced by the method is described in more detail above. The method may be useful, for example, in producing plants having increased yields, for example, crop plants having improved grain yield, relative to control plants.

[0127] In some embodiments, a method may further comprise reducing or abolishing the expression or activity of a DA polypeptide and/or EOD1/BB protein in the plant cell or plant.

[0128] This may be carried out before, at the same time or after the incorporation of the nucleic acid which encodes the CYP78A polypeptide. For example, in some embodiments, the expression or activity of a DA polypeptide and/or EOD1/BB protein may be abolished or reduced in one or more plant cells which already incorporate the nucleic acid encoding the CYP78A polypeptide. In other embodiments, the nucleic acid encoding the CYP78A polypeptide may be incorporated into one or more plant cells which have abolished or reduced expression of a DA polypeptide and/or EOD1/BB protein.

[0129] A plant thus produced may comprise a heterologous nucleic acid which encodes a CYP78A polypeptide and may possess abolished or reduced expression or activity of a DA polypeptide and/or EOD1/BB protein in one or more of its plant cells.

[0130] The expression or activity of a DA polypeptide and/or EOD1/BB protein may be reduced or abolished as described above. For example, a method may comprise incorporating a heterologous nucleic acid into a plant cell by means of transformation, wherein the nucleic acid encodes a suppressor nucleic acid, such as a siRNA or shRNA, which reduces the expression of a DA polypeptide and/or EOD1/BB protein.

[0131] The heterologous nucleic acids encoding the CYP78A polypeptide and Da and/or EOD1/BB suppressor nucleic acid may be on the same or different expression vectors and may be incorporated into the plant cell by conventional techniques.

[0132] CYP78A6 polypeptides and CYP78A suppressor nucleic acids are described in more detail above.

[0133] In some embodiments, the expression or activity of two or more CYP78A polypeptides, such as CYP78A6 and CYP78A9, may be abolished or reduced to produce a plant having reduced seed size.

[0134] A plant produced as described above may be sexually or asexually propagated or grown to produce off-spring or descendants. Off-spring or descendants of the plant regenerated from the one or more cells may be sexually or asexually propagated or grown. The plant or its off-spring or descendents may be crossed with other plants or with itself.

[0135] A plant suitable for use in the present methods is preferably a higher plant, for example an agricultural plant selected from the group consisting of Lithospermum erythrorhizon, Taxus spp, tobacco, cucurbits, carrot, vegetable brassica, melons, capsicums, grape vines, lettuce, strawberry, oilseed brassica, sugar beet, wheat, barley, maize, rice, soyabeans, peas, sorghum, sunflower, tomato, potato, pepper, chrysanthemum, carnation, linseed, hemp and rye.

[0136] In some embodiments, the plant may be a flowering plant (angiosperm). Flowering plants may include monocotyledons or dicotyledons, such as eudicots, in particular members of the Rosid clade, including Brasicaceae, such as broccoli, horseradish, cabbage, and cauliflower. In some embodiments, a plant may be other than Arabidopsis thaliana.

[0137] Another aspect of the invention provides a plant which comprises a heterologous nucleic acid which alters expression of a CYP78A polypeptide, as described above, and optionally has reduced or abolished expression of a DA polypeptide and/or EOD1/BB polypeptide.

[0138] The plant may display an altered seed size phenotype relative to controls (e.g. non-transgenic plants of the same species). For example, a plant which displays increased expression of a CYP78A polypeptide may display increased seed size relative to controls.

[0139] A plant which displays increased expression of the CYP78A6 polypeptide may also display one or more of; increased flower and leaf size, increased stem thickness, and increased height relative to control plants (e.g. identical plants which do not display increased expression of the CYP78A6 polypeptide).

[0140] A plant which displays reduced expression of a CYP78A polypeptide may display reduced seed size relative to controls.

[0141] A suitable plant with altered expression of a CYP78A polypeptide may be produced by a method described herein

[0142] In some embodiments, the plant may have normal fertility relative to control plants.

[0143] In some embodiments, a plant may not display increased organ size relative to controls.

[0144] In addition to a plant comprising a heterologous nucleic acid which alters CYP78A expression, for example a nucleic acid which encodes a CYP78A polypeptide or CYP78A suppressor molecule, as described herein, the invention encompasses any clone of such a plant, seed, selfed or hybrid progeny and descendants, and any part or propagule of any of these, such as cuttings and seed, which may be used in reproduction or propagation, sexual or asexual. Also encompassed by the invention is a plant which is a sexually or asexually propagated off-spring, clone or descendant of such a plant, or any part or propagule of said plant, off-spring, clone or descendant.

[0145] "and/or" where used herein is to be taken as specific disclosure of each of the two specified features or components with or without the other. For example "A and/or B" is to be taken as specific disclosure of each of (i) A, (ii) B and (iii) A and B, just as if each is set out individually herein.

[0146] Unless context dictates otherwise, the descriptions and definitions of the features set out above are not limited to any particular aspect or embodiment of the invention and apply equally to all aspects and embodiments which are described.

[0147] Having generally described the invention above, certain aspects and embodiments of the invention will now be illustrated by way of example to extend the written description and enablement of the invention, and to ensure adequate disclosure of the best mode of practicing the invention. Those skilled in the art will appreciate, however, that the scope of this invention should not be interpreted as being limited by the specifics of these examples. Rather, variations, extensions, modifications and equivalents of these specifics and generic extensions of these details may be made without departing from the scope of the invention comprehended by this disclosure. Therefore, for an appreciation of the scope of this invention and the exclusive rights claimed herein, reference should be had to the claims appended to this disclosure, including equivalents thereof.

[0148] All documents mentioned in this specification are incorporated herein by reference in their entirety for all purposes.

[0149] The contents of all database entries mentioned in this specification are also incorporated herein by reference in their entirety. This includes the versions of any sequences which are current at the filing date of this application.

Examples

[0150] Methods

[0151] Activation Tagging Screening

[0152] The Agrobacterium tumefaciens strain GV3101 was transformed with the activation tagging vector pJFAT260 (Fan et al., 2009), and the resulting strain was used for floral dip transformation of Arabidopsis da1-1 mutant plants (Li et al., 2008). T.sub.1 plants were selected by using the herbicide Basta. Seeds produced from T.sub.1 plants were passed through a fine wire sieve (425 .mu.m) (Fisher Scientific). Seeds retained by the sieve were kept for further characterization.

[0153] Plant Materials and Growth Conditions

[0154] Arabidopsis thaliana Columbia (Col-0) was the wild type line used. All mutants were in the Col-0 background. Plant materials and growth conditions are available in the Supporting Information.

[0155] Morphological and Cellular Analysis

[0156] Area measurements of fully expanded cotyledons, petals (stage 14), and leaves were made by flattening the organs, scanning to produce a digital image, and then calculating area by using Image J software. Embryo cell sizes were measured on the adaxial side of cotyledons from DIC images.

[0157] For analysis of whole-mount seeds, seeds were dissected from siliques and placed in a drop of clearing solution [30 ml H.sub.2O, 80 g Chloral hydrate (Sigma, C8383), 10 ml 100% Glycerol (Sigma, G6279)]. Samples were photographed under a Leica microscope (LEICA DM2500) with differential interference contrast optics using a SPOT FLEX Cooled CCD Digital Imaging System.

[0158] Seed Size and Seed Mass Analysis

[0159] Average seed mass was determined by weighing mature dry seeds in batches of 500 using an electronic analytical balance (METTLER TOLEDO AL104, China). The weights of five sample batches were measured for each seed lot. The wild-type and mutant seeds were photographed under a Leica microscope (LEICA S8APO) using Leica CCD (DFC420). The length, width and projective area of wild-type and mutant seeds were measured by using Image J software.

[0160] Cloning of the EOD3 Gene

[0161] The flanking region of the T-DNA insertion of the eod3-JD mutant was isolated by the thermal asymmetric interlaced PCR (TAIL-PCR) (Liu et al., 1995). Detailed protocols are described in the Supporting Information.

[0162] Constructs and Transformation

[0163] The EOD3 CDS was subcloned into the PstI site of the binary vector 35S::pGreen to generate the transformation plasmid 35S::EOD3. The specific primers for the EOD3 CDS are EOD3CDS-F and EOD3CDS-R.

[0164] The 1878 bp EOD3 promoter was subcloned into SacI and NcoI sites of the binary vector pGreen-GUS to generate the transformation plasmid pEOD3::GUS. The specific primers for the EOD3 promoter are EOD3PROM-F and EOD3PROM-R.

[0165] GUS Staining

[0166] Samples (pEOD3::GUS) were stained in a solution of 1 mM X-gluc, 50 mM NaPO.sub.4 buffer, 0.4 mM each K.sub.3Fe(CN).sub.6/K.sub.4Fe(CN).sub.6, 0.1% (v/v) Triton X-100 and incubated at 37.degree. C. for 8 hrs. After GUS staining chlorophyll was removed using 70% ethanol.

[0167] RT-PCR, Quantitative Real-Time RT-PCR, and RNA In Situ Hybridization

[0168] Total RNA was extracted from Arabidopsis seedlings using an RNAprep pure Plant kit (TIANGEN). Reverse transcription (RT)-PCR was performed as described (Li et al., 2006). cDNA samples were standardized on actin transcript amount using the primers ACTIN7-F and ACTIN7-R. Quantitative real-time RT-PCR analysis was performed with a Lightcycler 480 machine (Roche) using the Lightcycler 480 SYBR Green I Master (Roche). ACTIN2 mRNA was used as an internal control, and relative amounts of mRNA were calculated using the comparative threshold cycle method. RNA in situ hybridization method is described in the Supporting Information. The primers used for RT-PCR, quantitative real-time RT-PCR, and RNA in situ hybridization are described herein.

[0169] Plant Materials and Growth Conditions

[0170] Arabidopsis thaliana Columbia (Col-0) was the wild type line used. All mutants were in the Col-0 background. eod3-1D was identified as an enhancer of da1-1 by using T-DNA activation tagging method. The eod3-ko1 (CS833552), eod3-ko2 (CS806696), cyp78a9-ko1 (SALK 121278) and ttg2-3 (SALK 148838) were identified in AtIDB (Arabidopsis Thaliana Integrated Database) and obtained from Arabidopsis Stock Centre ABRC collection. The eod3-ko1, eod3-ko2, cyp78a9-ko1 and ttg2-3 mutants were backcrossed into Col-0 for three times. T-DNA insertions were confirmed by PCR and sequencing by using the primers described in Supplementary Table 3. Seeds were surface-sterilized with 100% isopropanol for 1 min and 10% (v/v) household bleach for 10 mins, washed at least five times with sterile water, stratified at 4.degree. C. for 2d in the dark, dispersed on Murashige and Skoog medium (Sigma) supplemented with 0.9% agar and 1% glucose, and then grown at 22.degree. C.

[0171] Cloning of the EOD3 Gene

[0172] The flanking region of the T-DNA insertion of the eod3-1D mutant was isolated by the thermal asymmetric interlaced PCR (TAIL-PCR) (Liu et al., 1995). Genomic DNA was prepared by using buffer containing 50 mM Tris-HCL (pH8.0), 25 mM EDTA, 250 mM NaCl and 0.5% SDS. Approximately 100 ng of the genomic DNA of the eod3-1D da1-1 mutant was used to perform TAIL-PCR analysis according to a previously reported method (Liu et al., 1995). Briefly, TAIL-PCR utilizes three nested specific primers (OJF22, OJF23 and OJF24) within the T-DNA region of the pJFAT260 vector together with a shorter arbitrary degenerate primer (AD1) so that the relative amplification efficiencies of specific and non-specific products can be thermally controlled. The specific primers OJF22, OJF23 and OJF24 and an arbitrary degenerate (AD1) primer are described in Table S3. TAIL-PCR products were sequenced by using the primer OJF24.

[0173] Cellular Analysis

[0174] For resin sections, siliques were cut transversely into four pieces and fixed in 4% paraformaldehyde. The tissues were embedded in Technovit 7100 resin (Heraeus Kulzer, Germany), sectioned at 5 .mu.m thickness and stained with 0.05% toluidine blue.

[0175] RNA In Situ Hybridization

[0176] In situ hybridization was performed as described (Li et al., 2003). DIG-labeled RNA transcripts were generated by transcription of EOD3 and CYP78A9 in sense or antisense orientation using SP6 or T7 RNA polymerase (Roche). After hybridization, washing and blocking, DIG-labeled RNA transcripts reacting with alkaline phosphatase-conjugated anti-DIG Fab fragment (1:3000 [v/v], Roche) were detected using 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium. The slides were observed with a microscope (LEICA DM2500) and photographed using a SPOT FLEX Cooled CCD Digital Imaging System.

[0177] Expression of Arabidopsis EOD3 in Oryza sativa

[0178] For overexpression of Arabidopsis EOD3 in Oryza sativa, the full length CDS of EOD3 was subcloned into pCR8/GW/TOPO TA cloning vector (Invitrogen) using TOPO enzyme and sequenced. The EOD3 CDS was then subcloned into Gateway Binary Vector pIPKb002 containing the Zm-ubiqutin promoter (FIG. 19). The construct was then introduced into callus of Oryza sativa L. japonica. cv. Nipponbare and selected on hygromycin-containing medium. The detailed methods were described in Hiei et al (1994) Plant J 6 271-282.

[0179] The areas of 24 seeds from each line of TO transgene plants were scanned to produce digital images, and then the average area per seed was calculated using Image J software as an indicator of seed size. The average area per seeds from each transgenic line was then determined.

[0180] Results

[0181] We previously characterized the Arabidopsis da1-1 mutant, which had larger seeds than wild type (Li et al., 2008). DA1, encoding a predicted ubiquitin receptor, sets final seed size by restricting cell proliferation (Li et al., 2008). To identify other components in the DA1 pathway or additional factors of seed size control, we initiated a T-DNA activation tagging screen in a da1-1 homozygous genetic background. Seeds produced from approximate 16,000 T.sub.1 plants were screened for mutations affecting the seed size phenotype of da1-1. A dominant enhancer of da1-1 (eod3-1D), which enhanced the seed size phenotype of da1-1, was identified (FIGS. 1A and D). Seeds of the eod3-1D da1-1 double mutant were dramatically larger and heavier than those of the da1-1 mutant (FIG. 1D an E). The embryo constitutes the major volume of a mature seed in Arabidopsis. The size of eod3-1D da1-1 embryos was substantially increased, compared with that of Col-0 and da1-1 embryos (FIG. 1B). The changes in seed size were also reflected in the size of seedlings (FIG. 1C). Cotyledons of eod3-1D da1-1 seedlings were significantly larger than those of Col-0 and da1-1 seedlings (FIGS. 1C and F). In addition, eod3-1D da1-1 double mutant had larger flowers and leaves than da1-1 (FIG. 10).

[0182] Eod3-1D Sets Large Seeds

[0183] To determine whether the single eod3-1D mutant has the altered seed size, we identified the eod3-1D mutant among F.sub.2 progeny derived from a cross between the eod3-1D da1-1 double mutant and wild type (Col-0). Seeds produced by eod3-1D were larger and heavier than the wild-type seeds (FIGS. 2A, E and F). In addition to the seed phenotype, eod3-1D plants showed larger flowers and leaves, thicker stems, and higher plants than wild type (FIG. 2B; FIG. 11A; Table 1). However, the number of rosette and cauline leaves was similar in wild type and eod3-1D, and the number of rosette and cauline branches in eod3-1D was also comparable with that in wild type (Table 1).

[0184] The eod3-1D mutation also caused defects in reproductive development. For example, eod3-1D mutant produced fewer elongated siliques than wild type (Table 1). First several flowers on the primary inflorescences of eod3-1D did not open normally (FIGS. 11B and C). Their stamens were much shorter than those of wild type (FIGS. 11B and C). The dehiscence of eod3-1D anthers was much delayed (FIG. 11C), but their pollens were functional (FIGS. 11D and E). The enlarged siliques were more frequently observed on the latest-arising flowers of old plants. In general, the enlarged siliques contained few seeds although the number of ovules per silique in eod3-1D was not reduced (Table 1). We observed that carpels of the late developing eod3-1D flowers were longer than those of wild-type flowers, whereas the length of stamens was similar to that of wild-type stamens (FIG. 2C; FIG. 11A), such that eod3-1D pollen is not able to directly reach stigmatic papillae; this could, in part, explain the decreased fertility. Fully elongated eod3-1D mutant siliques were longer and wider than wild-type siliques (FIG. 2D).

[0185] To determine whether the large seed size phenotype could result from allocation of extra resources to the few seeds produced, we hand-pollinated six flowers on primary inflorescences of wild-type plants, eod3-1D, and a male-sterile mutant (CS4002). For this set of experiments, flowers were pollinated with pollens of the same genotypes, with the exception of male-sterile plants for which wild-type pollens were used. Thus, each male-sterile plant produced only six siliques. The average seed size from male-sterile maternal plants was increased to 116% of that from wild-type maternal plants (FIG. 2G), indicating that seed size increased under condition of reduced fertility. By contrast, the average seed size from the eod3-1D mutant were approximate 170% of that from wild type (FIG. 2G), indicating that the effect of eod3-1D on seed size is not primarily due to its effect on fertility.

[0186] EOD3 Encodes a Cytochrome P450 Monooxygenase

[0187] To test whether this T-DNA insertion might cause the eod3-1D phenotypes, we analyzed the genetic linkage of the mutant phenotype with Basta resistance, which is conferred by the selectable marker of the activation tagging vector (Fan et al., 2009). All 101 plants with eod3-1D da1-1 phenotypes in T.sub.2 population were resistant, whereas the 36 plants with da1-1 phenotypes were sensitive, indicating that the insertion is responsible for the eod3-1D mutation. To identify the EOD3 gene, the DNA flanking the T-DNA insertion was isolated by thermal asymmetric interlaced PCR (Liu et al., 1995). Sequence analysis indicated that the insertion was in an intergenic region on chromosome II between the genes At2g46660 and At2g46670. The T-DNA had inserted approximately 3.2 kb upstream of the At2g46660 gene and about 6.5 kb downstream of the At2g46670 gene (FIG. 3A). The mRNA levels of these two genes were determined by reverse transcription-polymerase chain reaction (RT-PCR). Expression levels of the At2g46670 gene were similar in da1-1 and eod3-1D da1-1 plants (FIG. 3B), indicating that At2g46670 was unlikely to be the EOD3 gene. The mRNA of At2g46660 accumulated at a higher level in eod3-1D da1-1 than in da1-1 (FIG. 3B), strongly indicating that At2g46660 is likely to be the EOD3 gene. To demonstrate that this gene corresponded to EOD3, we overexpressed the At2g46660 gene in Col-0 wild-type plants and isolated 41 transgenic plants. Most transgenic plants showed large seeds and increased plant height (FIGS. 3C and D; FIG. 12A), as had been seen in the eod3-1D single mutant, confirming At2g46660 is the EOD3 gene. Importantly, the 35S::EOD3#7 transgenic plants exhibited normal growth and fertility, but produced significantly large seeds compared with wild type (FIG. 3 C; FIGS. 12B and C).

[0188] The EOD3 gene encodes the putative cytochrome P450 monooxygenase CYP78A6, one of six members of the CYP78A family in Arabidopsis. Genes in the CYP78A class belong to the group A cytochrome P450 in plants and seem to perform plant-specific functions (Zondlo and Irish, 1999; Ito and Meyerowitz, 2000; Anastasiou et al., 2007). EOD3/CYP78A6 exhibits the highest similarity to Arabidopsis CYP78A9 (FIG. 3E) (Ito and Meyerowitz, 2000).

[0189] EOD3/CYP78A6 Acts Redundantly with CYP78A9 to Control Seed Size

[0190] In order to further understand the function of EOD3, we isolated T-DNA inserted loss-of-function mutants for EOD3/CYP78A6 and CYP78A9, the most closely related family member. eod3-ko1 and eod3-ko2 were identified with T-DNA insertions in the first and second exons of the EOD3/CYP78A6 gene, respectively (FIG. 4A). cyp78a9-ko1 had T-DNA insertion in the second exon of CYP78A9 (FIG. 4B). The T-DNA insertion sites were confirmed by PCR using T-DNA specific and flanking primers and sequencing PCR products (FIG. 13). The eod3-ko1, eod3-ko2 and cyp78a9-ko1 mutants were further backcrossed into Col-0 for three times.

[0191] Seeds from eod3-ko1, eod3-ko2 and cyp78a9-ko1 mutants were smaller and lighter than seeds from wild-type plants (FIGS. 4F and G). Silique length in eod3-ko1, eod3-ko2 and cyp78a9-ko1 was reduced, compared with that in wild type (FIGS. 4D and H). By contrast, the size of leaves and petals, stem thickness and plant height in eod3-ko1 and cyp78a9-ko1 were comparable with those in wild type (Table 1). In addition, the number of rosette and cauline leaves, rosette and cauline branches, siliques per plant and ovules per silique in eod3-ko1 and cyp78a9-ko1 was similar to that in wild type (Table 1). As EOD3/CYP78A6 shows the highest similarity to the Arabidopsis CYP78A9, we postulated that EOD3 may act redundantly with CYP78A9 to control seed size. To test this, we generated the double knockout mutants, eod3-ko1 cyp78a9-ko1 and eod3-ko2 cyp78a9-ko1. The seed size and weight phenotype of eod3-ko mutants was synergistically enhanced by the disruption of CYP78A9 (FIGS. 4F and G), indicating that EOD3 functions redundantly with CYP78A9 to control seed growth. The eod3-ko cyp78a9-ko mutations also caused a significant change in seed shape (FIG. 4C). eod3-ko cyp78a9-ko seeds were shorter than wild-type seeds, whereas seed width was comparable with that of wild type (FIGS. 4C, I and J), indicating that eod3-ko cyp78a9-ko seeds are more round in shape than wild type. eod3-ko cyp78a9-ko produced fewer siliques per plant than wild type (Table 1). The length of siliques in eod3-ko cyp78a9-ko was dramatically reduced, compared with their parental lines (FIGS. 4D and H). Surprisingly, the number of ovules per silique in eod3-ko1 cyp78a9-ko1 was similar to that in wild type, resulting in a higher density of seeds within siliques (FIG. 4E; Table 1). In addition, the primary inflorescence stem of eod3-ko1 cyp78a9-ko1 was shorter than that of wild type, and the size of petals and leaves was slightly reduced compared with wild type (Table 1). However, the number of leaves and branches in eod3-ko1 cyp78a9-ko1 was comparable with that observed in wild type (Table 1).

[0192] EOD3 Acts Maternally to Influence Seed Size

[0193] To obtain clues about the genetic control of seed size, we asked whether EOD3 functions maternally or zygotically. Reciprocal cross experiments between wild type and eod3-ko1 cyp78a9-ko1 were performed. The effect of eod3-ko1 cyp78a9-ko1 on seed size was observed only when maternal plants are eod3-ko1 cyp78a9-ko1 mutant. Seeds produced by an eod3-ko1 cyp78a9-ko1 mother, regardless of the genotype of the pollen donor, were consistently smaller than those produced by maternal wild-type plants, and eod3-ko1 cyp78a9-ko1 mutant pollen in a wild-type mother produced seeds with wild-type size (FIG. 5A). This indicates that eod3-ko1 cyp78a9-ko1 can act maternally to control seed size. We further did reciprocal cross experiments between wild type and eod3-1D. Pollinating wild-type plants with eod3-1D pollen leads to the development of eod3-1D/+ embryos within a wild-type seed coat. However, the size of the resulting seeds was comparable with that of self-pollinated wild type seeds (FIG. 5B). In contrast, we could not observed the wild-type sized seeds from eod3-1D/+ plants fertilized with wild-type pollen, although half of them contained wild-type embryos. We further measured the size of individual seeds from eod3-1D/+ plants fertilized with wild-type pollen and genotyped the eod3-1D mutation. Our results show that the eod3-1D mutation is not associated with variation in the size of these seeds (FIG. 14). Together, these analyses indicate that the embryo and endosperm genotype for EOD3 do not influence seed size, and EOD3 is required in the sporophytic tissue of the mother plant to promote seed growth.

[0194] Eod3-Ko1 cyp78a9-Ko1 Reduces Cell Expansion in the Integuments of Developing Seeds

[0195] The reciprocal crosses indicate that EOD3 acts maternally to influence seed growth. The integuments surrounding the ovule form the seed coat after fertilization, which may physically restrict seed growth. The integument size of ovules is known to influence seed size (Garcia et al., 2005; Schruff et al., 2006). We therefore asked whether EOD3 functions through the maternal integument to affect seed size. To test this, we characterized mature ovules from wild type and eod3-ko1 cyp78a9-ko1 at 2 days after emasculation. Surprisingly, the size of eod3-ko1 cyp78a9-ko1 ovules was not significantly altered, compared with that of wild-type ovules (FIG. 6A and FIG. 15). We further investigated the outer integument length of wild-type and eod3-ko1 cyp78a9-ko1 seeds at specific times after pollination. The size of wild-type and eod3-ko1 cyp78a9-ko1 outer integuments showed a significant difference at 2 days after pollination (DAP) and subsequent time points (FIG. 6B). Previous study showed that the integument of a developing seed could completely stop cell division at 4 d after pollination (Garcia et al., 2005). To assess the contribution of cell proliferation and cell expansion in the integuments of developing seeds to eod3-ko1 cyp78a9-ko1, we measured outer integument cell number and cell size at 6 DAP. Outer integument cell number in eod3-ko1 cyp78a9-ko1 was similar to that in wild type (FIG. 6C), whereas cells in eod3-ko1 cyp78a9-ko1 outer integuments were significantly smaller than those in wild-type outer integuments (FIG. 6D). These results indicate that eod3-ko1 cyp78a9-ko1 restricts cell expansion in the integuments of developing seeds.

[0196] Eod3-1D Promotes Both Cell Proliferation and Cell Expansion in the Integuments

[0197] As the gain-of-function eod3-1D mutant had large seeds, we further asked whether eod3-1D mutant affects the integument size of ovules and developing seeds. The size of eod3-1D ovules was significantly larger than wild-type ovules (FIG. 6A and FIG. S6). eod3-1D also had dramatically larger outer integuments than wild type during the whole process of seed development (FIG. 6B). We further investigated outer integument cell number and cell size of developing seeds at 6 DAP and found that eod3-1D had more and larger outer integument cells than wild type (FIGS. 6 C and D).

[0198] Effects of Eod3-Ko1 cyp78a9-Ko1 and Eod3-1D Mutations on Embryo Development

[0199] eod3-ko1 cyp78a9-ko1 and eod3-1D had smaller and larger seed coats, respectively. The maternal integument or seed coat acts as a physical constraint on embryo growth. We therefore investigated whether eod3-ko1 cyp78a9-ko1 and eod3-1D integuments could indirectly influence embryo development. To test this, we manually pollinated wild-type, eod3-ko1 cyp78a9-ko1 and eod3-1D plants with their own pollen grains and examined developing embryos at specific times after pollination. In the siliques of wild-type plants, the majority of embryos reached the globular stage at 2DAP, the heart and torpedo stages at 4DAP, the bent-cotyledon stage at 6 DAP, and the stage of the fully filled seed cavity from 10 DAP onward (Table 2). Developmental progresses of eod3-ko1 cyp78a9-ko1 embryos were almost similar to those of wild type. However, morphological development of eod3-1D embryos was slightly slower than wild type at 4 DAP. At 6 DAP, most embryos reached the bent-cotyledon stage, as seen in wild-type plants (Table 2). This phenomenon of embryo development has also been observed in other Arabidopsis mutants (Schruff et al., 2006; Ohto et al., 2009; Zhou et al., 2009). Interestingly, the majority of wild-type embryos fully filled the seed cavity at 12 DAP, while most eod3-1D embryos completely filled the seed cavity at 14 DAP. It is plausible that eod3-1D forms a larger seed cavity than wild type; therefore eod3-1D embryos need to grow for a longer period of time to fill the large seed cavity than wild-type embryos.

[0200] Effects of Eod3-Ko1 cyp78a9-Ko1 and Eod3-1D Mutations on Embryo Cell Number and Cell Size

[0201] We isolated and visualized embryos from mature eod3-ko1 cyp78a9-ko1 and eod3-1D seeds. eod3-ko1 cyp78a9-ko1 embryos were significantly smaller than those of wild type, whereas eod3-1D produced large mature embryos compared with wild type (FIG. 7A). The average cotyledon area of eod3-ko1 cyp78a9-ko1 and eod3-1D embryos was about 72% and 196% that of wild-type embryos, respectively (FIG. 7B). The size of embryos is determined by both cell number and cell size. We measured palisade cells in the central regions of wild-type, eod3-ko1 cyp78a9-ko1 and eod3-1D cotyledons to learn which parameter is affected. The average size of eod3-ko1 cyp78a9-ko1 cotyledon cells was 79% that of wild-type cotyledon cells, while the average size of eod3-1D cotyledon cells was 1.36-fold that of the wild-type cotyledon cells (FIG. 7C). The magnitude of the changes in the areas of eod3-ko1 cyp78a9-ko1 and wild-type cotyledons (0.72 times) closely parallels the differences in the areas of cotyledon cells (0.79 times), indicating that eod3-ko1 cyp78a9-ko1 mainly affects embryo cell expansion. Given differences in the areas of eod3-1D and wild-type cotyledons (1.96 times) and cells (1.36 times), we conclude that eod3-1D had approximate 1.44 times more cells than wild type (1.96/1.36=1.44). These results indicate that eod3-ko1 cyp78a9-ko1 formed small embryos as a result of the reduced embryo cell expansion, and eod3-1D had large embryos due to increases in both embryo cell proliferation and cell expansion. Thus, EOD3 could act maternally to influence embryo cell proliferation and cell expansion because EOD3 is solely required in the sporophytic tissue of the mother plant to control seed growth (FIG. 5).

[0202] Expression Pattern of EOD3/CYP78A6

[0203] To examine the expression pattern of EOD3, RT-PCR were performed with total RNA from various tissues with EOD3-specific primers, including roots, stems, leaves, seedlings and inflorescences. EOD3 mRNA can be detected in all plant organs tested (FIG. 8A). To monitor EOD3 expression pattern during development, the pEOD3::GUS vector was constructed and transformed to wild-type plants. Tissues at different development stages were stained with GUS solution. In 14-d-old seedlings, GUS activity was detected in leaves. Relatively high GUS activity was observed in old leaves than in young ones (FIG. 8B). In flowers, GUS expression was detected in sepals, petals, stamens and carpels (FIG. 8C-H). Surprisingly, there was no EOD3 expression during the development of seeds (FIG. 8I-L; FIG. 17). We further performed in situ hybridization experiments to investigate expression of EOD3. EOD3 accumulated in the medial gynoecial domains at stage 8 (FIG. 8M). During stage 12, the EOD3 transcript was found within the central region of the septum (FIG. 8N). Expression was also seen in the funiculus (FIG. 8N). However, EOD3 expression was not detected in integuments, embryos, and endosperms during seed development (FIG. 16A-D), consistent with the GUS staining results. Similarly, CYP78A9 was also not observed in developing seeds (FIG. 16E-H). These analyses indicate that EOD3 is a temporally and spatially expressed gene.

[0204] EOD3 May Function Independently of DA1 and TTG2 to Influence Seed Size

[0205] da1-1 mutant had large seeds due to the increased cell proliferation in maternal integuments (Li et al., 2008), while eod3-ko mutants produced small seeds as a result of the reduced cell expansion in the integuments after fertilization, providing indication that EOD3 and DA1 might function in different pathways. However, the gain-of-function eod3-1D mutant promotes both cell proliferation and cell expansion in the integuments. We therefore asked whether there are any genetic interactions between eod3-1D and da1-1. To test this, we measured the size of seeds in wild-type, da1-1, eod3-1D and eod3-1D da1-1 plants. The genetic interaction between eod3-1D and da1-1 was essentially additive for seed size, compared with their parental lines (FIG. 9A), further indicating that EOD3 might function independently of DA1 to control seed size.

[0206] The TTG2 gene acts maternally to promote cell expansion in the integuments. ttg2 mutants produced small seeds as a result of the reduced cell elongation in the integuments (Garcia et al., 2005). To determine the genetic interaction between EOD3 and TTG2, we generated ttg2-3 eod3-ko1 double mutant. The genetic interaction between eod3-ko1 and ttg2-3 was additive for seed size, compared with their parental lines (FIG. 9B), providing indication that EOD3 functions to control seed growth separately from TTG2.

[0207] EOD3 Promotes Seed Growth by Increasing Maternal Integument Size

[0208] In this study, we identified the role of EOD3/CYP78A6 in seed size control. eod3-1D gain-of-function mutant formed larger seeds, while eod3-ko loss-of-function mutants exhibited smaller seeds. In addition, mutations in its most closely related family member CYP78A9 synergistically enhanced the seed size phenotype of eod3-ko mutants (FIGS. 4C, F and G), indicating that EOD3/CYP78A6 acts redundantly with CYP78A9 to influence seed growth. However, the eod3-1D mutant exhibited partial sterility although eod3-ko mutants had normal fertility. The tradeoff between seed number and size in many species (Harper et al., 1970), including Arabidopsis (Alonso-Blanco et al., 1999), has been observed. Our results show that the effect of eod3-1D on seed size is not primarily due to its effect on fertility. Similarly, recent studies show that apt and arf2 mutations increase seed size partly because of reduced fertility but also through a separate maternal effect on seed growth (Jofuku et al., 2005; Ohto et al., 2005; Schruff et al., 2006).

[0209] Reciprocal cross experiments show that EOD3 acts maternally to affect seed growth. The integuments surrounding the ovule are maternal tissues and form the seed coat after fertilization. Altered maternal integument size such as those seen in arf2, da1-1 and klu ovules is known to contribute to changes in seed size (Schruff et al., 2006; Li et al., 2008; Adamski et al., 2009). However, the size of mature eod3-ko1 cyp78a9-ko1 ovules was similar to that of wild-type ovules, indicating that the size difference between the wild-type and eod3-ko1 cyp78a9-ko1 seeds happens after fertilization. Consistent with this idea, eod3-ko1 cyp78a9-ko1 integuments were smaller than wild-type integuments from 2DAP onward (FIG. 6B). By contrast, eod3-1D formed large integuments in mature ovules and developing seeds (FIGS. 6A and B). Thus, a general theme emerging from these studies is that the control of maternal integument size is one of critical mechanisms for determining final seed size.

[0210] The size of the integument or seed coat is determined by cell proliferation and cell expansion. The cell number in the integuments of the mature ovule sets the growth potential of the seed coat after fertilization. For example, arf2 and da1-1 mutants had large ovules with more cells, resulting in large seeds (Schruff et al., 2006; Li et al., 2008), whereas klu mutants formed small ovules with less cells, leading to small seeds (Adamski et al., 2009). After fertilization, cells in integuments mainly undergo expansion. Our results indicate that eod3-ko1 cyp78a9-ko1 mutant formed normal-sized ovules, but smaller developing seeds as a result of the reduced cell expansion in the integuments after fertilization (FIG. 6). However, eod3-1D promoted both cell proliferation and cell elongation in the integuments of developing seeds, resulting in large volume of a seed cavity. Therefore, integument growth is driven by both cell proliferation and cell expansion; these two processes are assumed to be coordinated. In addition, our reciprocal cross experiments provide a demonstration of maternal sporophytic control of embryo growth (FIG. 5; FIG. 7; FIG. 14). The maternal integument or seed coat, which acts as a physical constraint on embryo and endosperm growth, may set an upper limit to final seed size.

[0211] The CYP78A Family Members have Overlapping and Distinct Functions in Seed Growth

[0212] EOD3 encodes a cytochrome P450 CYP78A6, one of the CYP78A family members. The other CYP78A subfamily member genes have been isolated as growth regulators. Overexpression of CYP78A9, which is most closely related to EOD3/CYP78A6, induced large and seedless silique in Arabidopsis (Ito and Meyerowitz, 2000). To a certain extent, plants overexpressing EOD3/CYP78A6 and CYP78A9 exhibited similar growth phenotypes, such as large siliques and short stamens (FIGS. 2C and D) (Ito and Meyerowitz, 2000), indicating that these two genes might affect the same or related metabolic network. In line with this idea, our genetic analyses demonstrate that the cyp78a9-ko1 mutation synergistically enhanced the seed size phenotype of eod3-ko mutants (FIGS. 4C and F). This provides indication that EOD3 and CYP78A9 may have overlapping functions in seed size control.

[0213] Another CYP78A subfamily member KLU/CYP78A5 also affects seed size by promoting cell proliferation in the integuments of ovules (Adamski et al., 2009). klu mutants produced smaller seeds than wild type due to small ovules with less cells (Adamski et al., 2009). By contrast, eod3-ko1 cyp78a9-ko1 mutants did not significantly affect the size of ovules, but restricted cell expansion in the integuments of developing seeds. These findings provide indication that KLU may act in the cell proliferation phase at the early stages of integument development, and EOD3 mainly functions in the cell expansion phase at the later stages of integument growth.

[0214] EOD3 and CYP78A9 May Control Seed Growth in a Non-Cell-Autonomous Manner

[0215] Another interesting feature of the CYP78A subfamily members is to generate mobile factors mediating organ growth (Miyoshi et al., 2004; Anastasiou et al., 2007). Rice PLA1/CYP78A11 affected cell division in the shoot apical meristem (SAM), but CYP78A11 expression was not detected in the shoot apical meristem, suggesting that CYP78A11 most likely acts through its non-cell-autonomous function (Miyoshi et al., 2004). Arabidopsis CYP78A5 has been proposed to be involved in generating a mobile signal distinct from the classical phytohormones (Anastasiou et al., 2007). However, mobile growth substances remain to be discovered. Interestingly, EOD3 and CYP78A9 were not detected in the maternal integuments of developing seeds (FIG. S7) (Ito and Meyerowitz, 2000), but eod3-ko, cyp78a9-ko and eod3-ko cyp78a9-ko mutants produced small seeds (FIGS. 4C and F). This suggests that EOD3 and CYP78A9 might control seed growth in a non-cell-autonomous manner, as proposed for other CYP78A subfamily members (Miyoshi et al., 2004; Anastasiou et al., 2007). However, EOD3 expression was detected in other organs, such as leaves and carpels (FIGS. 8B and F), providing indication that EOD3 might promote leaf and carpel growth in a cell-autonomous manner. Several Arabidopsis mutants with large organs also exhibited large seeds (Schruff et al., 2006; Li et al., 2008), suggesting a possible link between organ size and seed growth. By contrast, several other mutants with large organs produced normal-sized seeds (Szecsi et al., 2006; White, 2006), indicating that organ size is not always positively related to seed growth. 35S::EOD3#7 plants exhibited normal growth and fertility, but produced significantly larger seeds than wild type (FIGS. 3C and D; FIG. 12), providing indication that the effect of EOD3 on seed size might not be due to its effect on organ size. CYP78A9 has been suggested to be involved in producing an undiscovered plant growth substance (Ito and Meyerowitz, 2000). One of the functions of EOD3 might be production of a signal that promotes integument growth. Eventually, the elucidation of the biochemical function of these gene products may lead to the discovery of one or more new plant growth substances with use in control of seed size.

[0216] EOD3 Controls Seed Growth in Oryza sativa

[0217] The Arabidopsis EOD3 coding sequence was sub-cloned and overexpressed in Oryza sativa. The areas of the seeds from TO transgenic Oryza sativa plants were then determined.

[0218] The transgenic rice plant lines (To) showed a range of different average seed sizes (FIG. 20), as expected from variations in insertion site, expression levels and other factors. However, statistically significant differences in seed size were found between a number of the To lines and the smallest seed line (11-5) (FIG. 20; P<0.01). These statistically significant differences show that that Eod3 overexpression increases seed size in rice.

REFERENCES

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TABLE-US-00003

[0253] TABLE 1 Phenotypes of wild-type, eod3-ko1, eyp78a9-ko1, eod3-ko1 cyp78a9-ko1 and eod3-1D plants cyp78a9- eod3-ko1cyp78a9- Col-0 eod3-ko1 ko1 ko1 eod3-1D Petal area (mm.sup.2) 1.99 .+-. 0.11 1.99 .+-. 0.09 1.95 .+-. 0.10 1.86 .+-. 0.09** 2.48 .+-. 0.16** Petal length (mm) 3.08 .+-. 0.10 3.08 .+-. 0.09 3.05 .+-. 0.12 3.02 .+-. 0.08** 3.32 .+-. 0.11** Petal width (mm) 1.03 .+-. 0.04 1.03 .+-. 0.04 1.02 .+-. 0.04 1.01 .+-. 0.03** 1.18 .+-. 0.05** Leaf area (cm.sup.2) 1.02 .+-. 0.07 1.01 .+-. 0.07 0.99 .+-. 0.08 0.91 .+-. 0.08** 1.34 .+-. 0.11** Leaf length (cm) 1.29 .+-. 0.05 1.28 .+-. 0.04 1.27 .+-. 0.05 1.24 .+-. 0.07* 1.40 .+-. 0.09** Leaf width (cm) 1.01 .+-. 0.05 1.00 .+-. 0.04 0.99 .+-. 0.03 0.92 .+-. 0.04** 1.16 .+-. 0.05** Plant height (cm) 36.8 .+-. 1.9 36.7 .+-. 1.7 36.6 .+-. 1.5 33.5 .+-. 1.8** 64.8 .+-. 3.0** Stem thickness (mm) 0.88 .+-. 0.03 0.87 .+-. 0.03 0.87 .+-. 0.02 0.87 .+-. 0.04 0.99 .+-. 0.03** Number of RI 3.0 .+-. 0.7 3.0 .+-. 0.6 2.9 .+-. 0.6 2.9 .+-. 0.6 3.0 .+-. 0.6 Number of RII 4.0 .+-. 1.3 4.0 .+-. 0.9 3.9 .+-. 1.3 3.9 .+-. 1.1 4.0 .+-. 0.9 Number of CI 2.5 .+-. 0.5 2.5 .+-. 0.5 2.4 .+-. 0.5 2.4 .+-. 0.5 2.5 .+-. 0.5 Number of CII 5.0 .+-. 0.8 5.0 .+-. 1.0 4.9 .+-. 1.0 4.9 .+-. 1.1 5.0 .+-. 0.8 Leaf number 12.7 .+-. 0.8 12.6 .+-. 0.7 12.6 .+-. 0.7 12.6 .+-. 0.5 12.8 .+-. 0.7 Elongated siliques 328 .+-. 28 326 .+-. 32 324 .+-. 34 269 .+-. 22** 87 .+-. 18** % fertile siliques 99.1 .+-. 1.6 99.0 .+-. 1.5 98.9 .+-. 1.7 99.0 .+-. 1.8 21.1 .+-. 11.8** Ovule number per 53.9 .+-. 2.3 53.7 .+-. 2.6 53.4 .+-. 2.3 52.9 .+-. 2.1 53.1 .+-. 2.9 silique Silique fertility 98.4 .+-. 4.2% 98.2 .+-. 4.8% 98.2 .+-. 4.3% 98.1 .+-. 3.9% 51.8 .+-. 26.0%** Number of primary rosette branches (RI), second rosette branches (RII), primary cauline branches (CI), and second cauline branches (CII) were counted at 30 d after bolting. Primary rosette branches (RI) are axillary branches from rosette leaves, and second rosette branches (RII) are axillary branches from RI. Similarly, primary cauline branches (CI) are axillary branches from cauline leaves, and second cauline branches (CII) are axillary branches from CI. Opened flowers on the primary inflorescence were used to investigate ovules per silique. The elongated siliques on the primary inflorescence were used to investigate fertility. All values are given as mean .+-. SD. **P < 0.01 and *P < 0.05 compared with the wild type (Student's t-test).

TABLE-US-00004 TABLE 2 Developmental stages of embryogenesis The stage of the Quadrant or Bent fully filled DAP Genotype octant Dermatogen Globular Transition Heart Torpedo cotyledon seed cavity 2 Col-0 9 23 67 e3a9 8 21 65 eod3-1D 14 29 52 4 Col-0 5 36 72 e3a9 9 39 62 eod3-1D 17 28 40 13 6 Col-0 41 88 e3a9 29 97 eod3-1D 34 86 8 Col-0 7 70 e3a9 4 72 eod3-1D 18 80 10 Col-0 80 28 e3a9 77 13 eod3-1D 98 12 Col-0 10 90 e3a9 17 70 eod3-1D 89 14 Col-0 96 e3a9 124 eod3-1D 26 68 Siliques from the wild-type (Col-0), eod3-ko1cyp78a9-ko1 (e3a9) and eod3-1D plants were dissected. The number of embryos at each developmental stage was recorded.

TABLE-US-00005 TABLE 3 Name Primers Primers for verifying T-DNA eod3-1D-LP GGTCTAAGATTTCTCTCGTGTC (SEQ ID NO: 98) eod3-1D-RP CGTACGTCTTCTATTACTCCAC (SEQ ID NO: 99) CS833552LP AACTCCAAAGGATCAACCCAC (SEQ ID NO: 100) CS833552RP CCGGTTAAAGAATCGGCTTAC (SEQ ID NO: 101) CS806696LP GACTTGCAAAGATCGTTCACC (SEQ ID NO: 102) CS806696RP ACTCAATGTGACGTGTTGTGG (SEQ ID NO: 103) SALK121278LP TTTGATCGAGTGGATTCTTGC (SEQ ID NO: 104) SALK121278RP ATATTTGCTTGTAATCGGGGC (SEQ ID NO: 105) SALK148838LP(TTG2) TAAAACCAAACGACACCGTTC (SEQ ID NO: 106) SALK148838RP(TTG2) TCCAAGTTTGTTGACGATTCC (SEQ ID NO: 107) OJF22 CGAGTATCAATGGAAACTTAACCG (SEQ ID NO: 108) OJF23 AACGGAGAGTGGCTTGAGAT (SEQ ID NO: 109) OJF24 TGGCCCTTATGGTTTCTGCA (SEQ ID NO: 110) AD1 NTCGA(G/C)T(A/T)T(G/C)G(A/T)GTT (SEQ ID NO: 111) SALK_LBa1 TGGTTCACGTAGTGGGCCATCG (SEQ ID NO: 112) SAIL_LB2 GCTTCCTATTATATCTTCCCAAATTACCAATACA (SEQ ID NO: 113) Primers for constructs EOD3CDS-F CTGCAGATGGCTACGAAACTCGAAAGCTCC (SEQ ID NO: 114) EOD3CDS-R CTGCAGTTAACTGCGCCTACGGCGCAATTT (SEQ ID NO: 115) EOD3PROM-F GAGCTCTGTCTCGTGGATAAGTAG (SEQ ID NO: 116) EOD3PROM-R CCATGGGGCGGATCAAAGCAAAGTAAG (SEQ ID NO: 117) Primers for RT-PCR EOD3RT-F ACCAACCTTGCCTTCTCC (SEQ ID NO: 118) EOD3RT-R CGTCTCGGCTCTTCTGATT (SEQ ID NO: 119) AT2G46670RT-F ACAACGAGCAGCAACCA (SEQ ID NO: 120) AT2G46670RT-R TCTTCAACCGGAACTTCAT (SEQ ID NO: 121) ACTIN7-F ATCCTTCCTGATATCGAC (SEQ ID NO: 122) ACTIN7-R GAGAAGATGACTCAGATC (SEQ ID NO: 123) Primers for quantitative real-time RT-PCR EOD3QRT-F CCGGTTAAAGAATCGGCTTA (SEQ ID NO: 124) EOD3QRT-R TTGAGATCACTCGTCGTTGC (SEQ ID NO: 125) ACTIN2-F GAAATCACAGCACTTGCACC (SEQ ID NO: 126) ACTIN2-R AAGCCTTTGATCTTGAGAGC (SEQ ID NO: 127) Primers for in situ hybridization EOD3INSITU-F AAAGAAGCTCATATGAGAATTA (SEQ ID NO: 128) EOD3INSITU-R TGGTGTAAATATAAATTGAAACT (SEQ ID NO: 129) CYP78A9INSITU-F TTAGTGTATGATAAGGCTAAGGCT (SEQ ID NO: 130) CYP78A9INSITU-R GTATTAACTTTTCTTTGTGACA (SEQ ID NO: 131)

Sequence CWU 1

1

13111593DNAArabidopsis thaliana 1atggctacga aactcgaaag ctccttaatc tttgcccttt tgtccaaatg cagcgttcta 60agccaaacca accttgcctt ctccctcctc gccgtcacaa tcatctggct cgccatatct 120ctcttcttat ggacctatcc cggtggacct gcttggggga aatacctctt cggccggtta 180atatccggtt catacaaaac cggaaacgtt attcccggtc caaaaggctt ccctttggtt 240ggaagcatgt cactcatgtc aagcactcta gctcaccgac gaatcgctga tgcagctgag 300aaattcggag ccaagaggct catggctttc agcttaggag agactcgcgt gatcgtcacg 360tgcaatcccg acgtagcgaa agagattctg aatagcccgg tttttgctga tcgaccggtt 420aaagaatcgg cttactcact gatgtttaac agagcaattg gttttgcacc acacggtgtt 480tactggcgaa cgcttcgccg tatcgcttcg aaccatctct ttagtacaaa acaaatcaga 540agagccgaga cgcaacgacg agtgatctca agccagatgg ttgagtttct tgaaaaacag 600agtagtaacg aaccctgttt tgttcgtgag ttgcttaaaa cggcgtcgct taacaacatg 660atgtgctctg tattcggaca agagtatgag cttgaaaaaa accatgttga gttacgtgaa 720atggtcgaag aaggttatga tttgctcgga acgttgaatt ggactgatca ccttccttgg 780ctatcggagt ttgatcctca aagactccgg tctagatgtt ccacactcgt accaaaggta 840aaccggtttg tatcccggat tatatccgaa caccgtaatc aaaccggtga tttgcctcgt 900gatttcgtcg acgttttgct ctccctccat ggttcagata aattatccga cccggacata 960atcgccgttc tttgggagat gatattcaga ggaacagaca cagttgcggt cttaatcgag 1020tggatcctcg ctaggatggt ccttcatcca gatatgcaat caacggtaca aaacgagctg 1080gatcaagtag tcgggaaatc aagagcccta gatgaatctg acttggcttc acttccatat 1140ctaacggctg tggtgaaaga agtattgagg cttcatcctc caggcccact tctatcatgg 1200gcccgtttgg ccataacaga cacgatcgtt gatggtcgtc ttgttccggc agggaccaca 1260gcaatggtga acatgtgggc cgtatcgcat gatccacacg tgtgggttga tcctttggag 1320tttaaacctg agaggttcgt ggcaaaagaa ggtgaggtgg agttttcggt tcttgggtcg 1380gatttgagac ttgcaccttt cgggtcgggt cgtcggattt gccccgggaa gaatcttggt 1440tttactaccg ttatgttttg gacggcgatg atgttacatg agtttgaatg gggaccgtcc 1500gatggtaacg gcgttgactt atctgagaaa ctgaggcttt cttgcgagat ggctaatcct 1560cttcctgcta aattgcgccg taggcgcagt taa 159321668DNAArabidopsis thaliana 2atggccacca agctcgacac cagtagctta cttttggccc tcttgtccaa atgtagcctc 60cttactcaaa ccaatcttgc tctctctctc ctcgtagcct ccctagcttc tctcgctctt 120tctctcttct tctggtctca tcccggagga cccgcatggg gaaagtactt cctccaccgc 180cgccgtcaaa ccaccgtgat acccgggcca agaggcttac cttttgtcgg aagcatgtct 240ctcatgtcaa acactctggc tcaccgttgc atagccgcaa ccgcagagaa atttagagcc 300gaacggttaa tggcgtttag tttgggagaa actcgcgtga tcgtcacgtg caatcctgat 360gtagctaaag agattctaaa cagtccggtt ttcgctgacc gcccggttaa ggaatcagct 420tattccctca tgtttaaccg tgctatcggt ttcgctcctt acggcgttta ctggcgaacc 480ttgagaaaaa tcgcgtctaa tcatcttttc agcccgaaac agattaaacg ttccgaaacg 540cagagaagcg tgatcgcgaa tcaaatcgtg aagtgtctca caaaacagag taacaccaaa 600ggtctctgtt tcgcacgtga cttgatcaaa acggcatcgc ttaataacat gatgtgctct 660gttttcggaa aagaatacga gcttgaggaa gagcatgaag aagtgagtga gctacgtgaa 720ttggtggaag aaggttatga tttactcggt acactgaatt ggaccgatca tctcccatgg 780ctctctgaat ttgatcctca aagaatccgg tctagatgct ctaatctcgt cccaaaagta 840aaccggtttg tgaaccggat tatctctgac caccgtgaac aaactcgtga ctcaccgagt 900gacttcgttg acgtattgct ctctctcgat ggtcctgata aattatccga ccctgatatc 960atcgccgttc tatgggaaat gatattcaga ggaactgaca cggtggctgt tttgatcgag 1020tggattcttg ctaggatggt ccttcatcca gatattcaat cgacggttca caatgagctt 1080gatcaaatcg tgggacgatc aagggctgtc gaagagtctg acgtggtgtc tctagtatat 1140ctaacggctg tggtgaaaga agtcttgagg cttcacccgc caggcccact actctcatgg 1200gcccgtttag caatcacaga cacgatcatc gacggtcgtc gtgttccggc ggggaccacc 1260gcaatggtga acatgtgggc tattgcacac gatccacacg tgtgggagaa tccgttggag 1320tttaaacccg aacgttttgt agccaaggaa ggtgaggttg agttctcggt tcttgggtct 1380gatttgaggc ttgcaccgtt cgggtccggt cgtcgggttt gccccgggaa gaatcttggt 1440ttgaccaccg tgacgttttg gactgcgacg cttttgcatg agtttgaatg gctgacgccg 1500tccgatgaga agaccgttga cttgtccgag aaactgaggc tctcgtgtga gatggctaat 1560cctcttgctg ctaaattacg ccccaggcgc agttttagtc aaaagaataa aataaagaac 1620aaagaaagta aaggaaacaa aaaaaaagaa tcatacaaaa aatactaa 166831608DNAArabidopsis thaliana 3atgagaaccg aaatagaaag tttgtgggta tttgctcttg catcaaaatt caatatttac 60atgcaacaac attttgcttc ccttctcgtc gccattgcta tcacttggtt taccataacc 120atcgtatttt ggtctactcc gggtggaccg gcttggggaa aatacttctt cactcgccgg 180tttatttctc tcgattacaa ccgaaaatac aagaatctca ttcccggtcc tagagggttt 240ccacttgtgg gaagcatgag ccttaggtca agccacgtgg ctcatcagcg catagcgtct 300gtggctgaga tgagtaacgc caagcggctc atggcgttta gcctcggtga tactaaggtg 360gtggtgacgt gtcatcctgc cgtggcaaag gagatactaa acagttcggt ttttgctgac 420cgaccggttg acgaaaccgc ttacggtttg atgtttaacc gagccatggg atttgctccc 480aatggtactt attggcgtac gctgcgtcgg ttaggctcga accatctttt taacccgaag 540caaatcaaac aatcggagga tcagagacgg gtgatagcga ctcagatggt gaatgcgttt 600gcacgtaacc ctaaatccgc gtgtgcagtg cgtgatttgc tcaaaacagc gtcgttgtgt 660aacatgatgg gtttggtttt cgggagagag tatgaattgg agtcaaataa caacttggaa 720tctgaatgct taaagggttt ggttgaagaa gggtacgatc ttctaggtac gttaaattgg 780accgaccatc ttccttggtt agccggttta gatttccaac aaatccggtt taggtgctcg 840cagctcgtac cgaaagtaaa tctgttattg agccgtatca tacatgaaca acgtgctgcc 900acgggtaact ttcttgacat gttactttct cttcaaggtt cagaaaaatt atcagaatcc 960gacatggttg ctgttctttg ggaaatgata tttaggggaa cggacactgt tgcggttttg 1020gtcgagtggg tgctagcgag gattgtgatg catcccaaag ttcaattaac ggtccacgat 1080gagcttgacc gagtcgttgg cagatcaaga accgtggatg agtcagacct tccatcactc 1140acgtatctaa cggctatgat caaagaagtg ttgaggctgc atccaccagg tccactgctt 1200tcttgggcac gactgtctat aacagacact tccgtagatg gatatcacgt gccggctggg 1260accaccgcga tggtcaacat gtgggctata gcacgtgacc cacacgtgtg ggaggatcct 1320ttagagttta agcctgagag gttcgtggct aaagagggtg aagctgagtt ctctgttttc 1380gggtcggatc tgaggttggc accgttcggg tcgggtaaga gggtttgccc tggaaagaat 1440ttgggactta caacggtgtc gttttgggtt gcaacgctct tgcatgagtt tgagtggctt 1500cctagcgtcg aagctaaccc tccagatctc tcggaggttt tgaggctctc gtgtgagatg 1560gcttgtccac tcatcgttaa cgtaagctca aggcgtaaga taatgtaa 160841611DNAArabidopsis thaliana 4atggagttga tgaatttggc ttcaaaagaa acaagctatt ggatgattgc actgcctgcc 60ggttttggat cccaaaacct acatgatgtt tccaccctag gctatctatt ccttgccgtc 120gtttttctct ctatagtcac gtgggctctc gccggaggcg gtggtgtcgc ttggaagaac 180ggccgtaacc ggttgggtcg tgtcgcgatc cctggtcctc gtggcatacc agtattcggc 240agtcttttca ctctcagccg aggcttggct catcggacgt tagcagccat ggcttggagc 300cgagccaaca ctgagattat ggcttttagc cttggttcaa cgccggttat cgtggcttct 360gaaccaaaca tagctcgtga gattctgatg tcgcctcact tcgcggaccg gccggttaag 420cagtctgcta agagcctcat gttcagccga gccataggtt tcgccccaaa cgggacttac 480tggcgcatgt taagaaggat cgcatcgact cacctatttg ctcctcggcg tatcttagca 540cacgaagctg ggcgccagct agactgcgct gaaatggtga aagctgtgtc agtggagcaa 600aacggcgctg gatcagtcgt tttaaggaaa cacttacaac tagccgcctt gaacaacatc 660atgggaagtg tttttgggag aagatacgat cctctggctc agaaagagga tcttgatgag 720cttacatcaa tggttaggga agggttcgag cttttgggtg cttttaattg gtctgattat 780cttccatggc tcggttattt ctacgactca attcgtttaa accaacgttg ctcagatctc 840gtccctcgaa ttagaaccct cgtcaagaaa atcatcgacg aacatcgagt tagtaactct 900gagaagaaaa gagacattgg agattttgtt gatgtcttat tgtctttaga cggtgatgag 960aaacttcaag aagatgacat gatcgccgtt ttatgggaga tgatttttcg agggacagat 1020acaacggcgt tattaacgga gtggaccatg gccgagctag tactgaaccc taacgtgcaa 1080accaagttac gagacgagat tttaactgct gtgggcgacg gcgccgacgg agacgtggca 1140gatgctgacc tggcaaaact cccgtaccta aacgcagtgg tgaaggaaac tctaaggctg 1200catcctcctg gaccactgct ttcatgggct cgtctttcca cgtcagacgt ccagctcagc 1260aatggcatgg tgattccaaa gggaactaca gcgatggtca acatgtgggc tataacccac 1320gaccagactg tatggtccga cccgctaaag tttgacccgg agagattcac tgggaatgct 1380gacatggata ttcgtggtgg ggatctaagg cttgcaccgt ttggagccgg taggagagtg 1440tgtccgggga agaacatggg gctagctact gtgactcggt gggtggctga gttggtacga 1500cggttcgagt ggggtcagga tcagaccgag ccagttgatc ttggtgaggt cttgaagctt 1560tcttgtgaga tggagcatcc gttacgtgcc gttgtaacgg aaatatttta a 161151554DNAArabidopsis thaliana 5atgtctccgg aagcttacgt tctgttcttt aacagtttta acctcgtaac cttcgaagcc 60tttgcttcag tctcacttat catagccaca gttgctttct tgctctcacc aggtgggctc 120gcatgggcct ggaccgggtc atccaagagt cgggtttcga ttccaggacc atctggttct 180ctttccgtct tctccggctc caatccccac cgtgttctcg ccgctcttgc taaacgcttc 240aaggcctctc cgttgatggc gttctcagtt gggttttcgc gtttcgttat ctctagtgaa 300ccggagacgg ctaaagagat tttgagcagc tctgcttttg ctgaccggcc ggttaaggag 360tcagcttacg agcttttgtt tcaccgtgcc atgggattcg caccgtatgg tgagtattgg 420aggaatctga ggagaatctc ctccactcat cttttcagtc caagaagaat cgcgagtttt 480gagggtgtta gagttggcat cggtatgaag atggtcaaga agattaaaag ccttgttacg 540tctgatgctt gtggtgaagt tgaagtgaaa aagatcgttc actttggttc tttgaataat 600gtaatgacga cagtgtttgg tgaaagctac gattttgatg aagttaatgg aaaagggtgt 660tttttggaga ggctggtgag tgaaggctac gagttgcttg ggatttttaa ctggagtgat 720cacttttggt ttcttcgttg gtttgacttc caaggagtga ggaagaggtg tagagctttg 780gtctctgaag tcaacacttt tgtcggcgga ataattgaga aacacaagat gaagaagggt 840aataatctca atggagagga aaatgacttc gttgatgtct tgcttggctt gcaaaaggat 900gaaaagttgt ctgattctga catgattgct gttctttggg aaatgatatt tagagggaca 960gatacagttg cgattctagt ggaatgggtg cttgcaagaa tggttttgca tcaagacatc 1020caagataaac tctacagaga gatagcttct gctacaagta acaatattag atccttgtct 1080gattccgaca tcccaaaact gccgtacctt caagctattg tcaaagaaac cctaaggctc 1140cacccccctg gtccacttct ctcttgggct cgtctcgcta tccatgacgt ccacgtaggt 1200cctaaccttg tccctgctgg aaccatagct atggtcaaca tgtggtccat cacacacaac 1260gctaaaatct ggaccgaccc tgaagcgttt atgcctgaaa ggttcattag tgaggatgtg 1320agcatcatgg gctcggatct tagattggct ccattcggat ccggtcgtcg ggtttgtccc 1380ggtaaagcaa tgggtctagc tactgttcat ctctggattg gtcaactaat tcagaatttt 1440gaatgggtga agggttcttg tgatgttgag ctcgctgagg ttctgaagct gtctatggag 1500atgaagaatc cgttgaagtg caaggctgtt ccaaggaatg ttggtttcgc ttga 155461614DNAArabidopsis thaliana 6atgactattg atatgtatct ttccttcgct tctcgttctg gatcttctcc atttccaagt 60ctcgagcttt gtctcagcat tttcctcttc atctcccttt tcgttttctg gttgactcca 120ggtggctttg cttgggcact ctacaaagct cgtttccata cccgacctga gtccaaaacc 180ggacctgcca ttcccggccc gtctggtctc cccatctttg gcctcctctt ggcttttgtc 240aacaacgcct taacacacag aatcctcgcc aatattgctg acacttgcaa agcaaaagct 300ctcatggcgt tctccgtagg gtcaacccgg tttgttataa ccagcgaacc agagaccgcg 360aaagagcttc taaacagctc tgcttttgca gaccggccag tgaaagagtc tgcttacgag 420ctgctctttg atagagccat ggggtttgct ccctttggtg attactggag agagttgagg 480agaatctctt ctacccatct cttcagccct aagaggatct tcagttctgg cgagtcccgc 540cgaaaaatcg ggcaaaacat ggtaggagag atcaagaacg caatggagtg ttatggagaa 600gtgcatataa aaaagatctt gcatttcgga tcactcaaca acgtgatgtc tagcgttttc 660ggtaaaacat acaacttcaa cgaaggtatt gtctactcga aagagagcaa tgagttggag 720catttggtgt ctgaaggcta tgagctgctc ggaatcttca actggagtga tcatttccct 780ggaatgagat ggttagattt acaaggtgtg aggagaagat gtcgtagttt ggtcggtaga 840gtgaatgtgt tcgtcggtaa gataatcaat gaccacaaat caaagaggtc acttcgtgat 900aatcctgaag agagcactta tgatgatgac tttgtagatg tcttacttgg catgcacggc 960aacagcaaac tttctgactc cgatatgatc gcagtccttt gggaaatgat ttttagggga 1020acagacacgg tggcgattct cttggaatgg atccttgcga ggatggttct tcaccctgac 1080attcaagcca aggcgcaggc cgagatcgat tgtatcgtgg gtgactcggg acgtcaagtc 1140acagactcag acctccccaa gctcccatac gttcgtgcca ttgtcaagga aaccctaagg 1200atgcacccac ctggtcctct cctctcatgg gctcgtctct ccattcacga tactcagatc 1260gggactcact ttatacccgc aggaaccact gcgatggtta acatgtgggc tataacccac 1320gatgaaaagg tctggccgga agctcatgag tataaaccag agaggtttct tggtgcgcaa 1380gaaagtaata acttccccat catgggatct gatctgaggc ttgctccctt cggtgctgga 1440cgtagggtct gtcccggcaa gtcaatgggt ctagccaccg tggagctatg gctagctcag 1500ttgctaggaa gctataagtg ggtctcatgt ggtgaagtgg atttgagtga gactttgaag 1560ctatctttgg agatgaagaa cactcttgtc tgcaaggcaa tccctagggg ttaa 161471668DNAOryza sativa 7atggcaatgg ccaccgccac cgcctcctcc tgcgtcgacg ccacgtggtg ggcgtacgcc 60ctcccggcgc tcctcggcgc cgacaccctc tgcgcccacc cggcgctgct cgccggcgcc 120gtcctcctgg ccttcgccac cgccgcggtg ctcgcctggg ccgcgtcccc cggcgggccg 180gcgtgggcgc acggccgcgg ccgcctcggc gcgacgccca tcgaggggcc ccgggggctc 240cccgtgttcg gcagcatctt cgcgctctcc cggggcctcc cgcaccgcgc gctcgacgcg 300atgtcgcgcg acgcggcggc gccacgggcg agggagctca tggcgttctc cgtcggggag 360acgccggcgg tggtgtcgtc gtgcccggcg acggcgaggg aggtgctcgc gcacccgtcg 420ttcgccgacc gcccgctgaa gcgctcggcg cgggagctgc tgttcgcgcg cgccatcggg 480ttcgccccca gcggcgagta ctggcgcctc ctccgccgca tcgcctccac ccacctcttc 540tcccctcgcc gcgtcgccgc gcacgagccg gggcgccagg ccgacgccac ggcgatgctg 600tccgccatgg ccgccgagca gtccgccacc ggcgccgtcg tgctccgccc ccacctccag 660gccgccgcgc tcaacaacat catgggcagc gtgttcggcc ggcgctacga cgtctcctcc 720tcctccggcg ccgccgccga cgaggccgag cagctcaaga gcatggtgcg cgaggggttc 780gagctcctcg gcgcgttcaa ctggtccgac cacctcccat ggctcgccca cctctacgac 840cccaaccacg tcgcccgccg ctgcgccgcg ctcgtccccc gcgtccaggc gttcgtccgc 900ggcgtcatcc gcgaccaccg cctccgccgc gactcctcct ccaccgccgc cgacaatgcc 960gacttcgtcg acgtcctcct ctccctcgag gcccacgaga acctcgccga ggacgacatg 1020gtcgccgtcc tctgggagat gatatttcgt gggacggaca cgacggcgtt ggtgacggag 1080tggtgcatgg cggaggtggt gaggaacccg gcggtgcagg cgaggctgag ggcggaggtg 1140gacgcggcgg tgggcggcga cgggtgtccc agcgacggcg acgtggcgcg gatgccgtac 1200ctgcaggcgg tggtgaagga gacgctgagg gcgcacccgc cggggccgct gctgagctgg 1260gcgcggctgg ccaccgccga cgtggggctc gccaacggca tggtggtgcc ggcgggcacg 1320acggcgatgg tgaacatgtg ggccatcacc cacgacggcg aggtgtgggc cgacccggag 1380gcgttcgcgc cggagcggtt catcccgtcg gagggcggcg ccgacgtcga cgtccgcgac 1440ggcgacctcc gcctggcgcc gttcggcgcc gggcgccgcg tctgccccgg caagaacctc 1500ggcctcgcca ccgtctccct ctgggtcgcc cgcctcgtcc acgccttcga ctggttcctc 1560cccgacggct cgccgccggt gtccctcgac gaggtcctca agctctccct cgagatgaag 1620acccctctcg ccgccgccgc caccccccgc cgccgccgcg ccgcctga 166882133DNAZea mays 8acccattcac tcactcatct caaggcctag gtagcaccgt agcagctact agaagcagct 60agccagaaca actcgtccat ggcgatggcc tccgcggctt gctcatgcac ggacggcacg 120tggtgggtgt acgcgctccc ggcgctgctc ggctccgaca ccctgtgcgc ccacccggcc 180ctcctggctg gcctgatctt tctggccacc gtctcggtgg ctctgctggc gtgggccacg 240tcgccgggcg gtccggcgtg gacgaacggc cgcggccgcc tcggcgtcac tcctatcgtg 300ggaccccgtg gtctgcccgt gttcggcagc atcttcgcgc tgtcccgcgg gctgccgcac 360cgcgccctcg ccgagatggc ccgcgccgca gggccccggg ccaaggagct catggcgttc 420tccgtcggtg acacgcccgc ggtcgtgtcg tcctgcccgg ccacggcacg tgaggtgctc 480gcgcacccgt cattcgccga ccgccctgtg aagcggtcgg cccgggagct catgttcgcg 540cgtgccatcg ggttcgcgcc caacggcgag tactggcgcc gcctccgccg cgtcgcgtcc 600acgcacctct tttccccgcg ccgggtcgcc tcgcacgagc cgggacgcca aggtgacgcg 660gaggccatgc tccgctccat cgccgccgag cagtcggcct ctggcgccgt cgccctccgc 720ccgcacctcc aggccgccgc tctcaacaac atcatgggca gcgtcttcgg cacgcggtac 780gacgtcacat caggcgccgg cgccgcggag gccgagcatc tcaagagcat ggtgcgcgag 840gggttcgagc tcctcggcgc cttcaactgg tccgaccacc tcccctggct cgcccacctg 900tacgacccaa gcaacgtcac ccgccggtgc gccgcgctcg tgccgcgcgt ccagaccttc 960gtccgtggcg tcatcgacga gcaccggcgc cgccgccaaa actccgccgc cctcaacgac 1020aatgctgact tcgtcgacgt gctcctctcc ctcgagggtg acgagaagct cggcgacgac 1080gacatggtcg ccatcctctg ggagatggtc ttccgcggta cggacacgac gacgcttctg 1140accgagtggt gcatggcgga gctggtgcgc cacccggcgg tgcaggcgag ggtgcgcgcc 1200gaggtcgacg cggctgtcgg tgccggaggt tgccccaccg acgccgacgt ggcgcgcatg 1260ccgtacctgc aggcggttgt gaaggagacg ctgcgcgccc acccgcctgg cccgctgctg 1320agctgggctc gcctcgccac cgccgacgtg ccactctgca acggcatggt ggtcccggct 1380ggcaccacgg cgatggtgaa tatgtgggcc ataacccacg atgccgccgt gtgggccgac 1440ccggacgcgt tcgcgccgga gcggttcctg ccctccgggg gcggcgccga cgtggacgtc 1500cgcggcgtcg acctccgcct ggccccgttc ggcgccgggc gtcgcgtctg ccccggcaag 1560aacctgggcc tcaccaccgt gggcctctgg gttgcccgcc tcgtgcacgc cttccagtgg 1620gccctgcctg acggcgcggc ggccgtttgc ctcgacgagg tcctcaagct ctccctggag 1680atgaagacgc cgctcgtcgc cgcagccatc ccccgcaccg cctgatccgt cctgccgccg 1740acgcgtcacg tcacgcgttg tttgcatgga tgatggtatc tttgtctgtc tgtgtggtct 1800tcgctaaagt ttgcttcttc tcgatcgtcg gttcgttcgt gcctccacct tagcctaggg 1860tttggtttct tgcaaggtag tgagtgtgtc ttagtctcac catcaccggg gctccaattt 1920tggaaagctg cgtgttagga gttaacccct agacatgttt gcgtcttgat cgccaccacc 1980catcagtatc agcgcagaaa ctacatatag atcagtgttt gtcgaccagt catggaagtc 2040gtgtgctctc aagtctgatg tattatatac atatatatgt attgtaatgt gattatcaag 2100aaccgtgcta tttacaaaaa aaaaaaaaaa aaa 213391799DNAPhalaenopsis sp. SM9108 9cggcaccact cctctctgtt cctctaatat ctggttaaaa atgacaatgt catccatgga 60ttcatcttca ataatattaa cttatctctc cccaacactt tctccagcta tcgccgcttc 120tatcatcatc atctcagctc tactactctt tcccggcggt ctggcgtggg ccctttccct 180caagcgccca acattctccg ggcccaccgg aattgttttt gctctcgcca gctctgctgc 240tcataagtca cttgccgccc tagctcgctc cgttcgacgc cctccgcctc atggctttct 300cggtcggcct cactcgcttc atcgtttcaa gccacccgaa aaccgcaaaa gagattcttt 360caagcccagc cttcgctgat cggcccatta aagaatcagc atacgaactt ctgtttaatc 420gcgctatggg ttttgcccca tttggggatt actggagaaa cctgagaagg atttcgtcca 480catatctttt cagtccgcgg cgagtttcat cgttcgagaa gcaacggagt gagattggcg 540aaggaatggt gcgggatatg aaaagaatga tggagagaaa tggagttgta gaagtgagga 600gaatgttgca ctacgggtct ttgaataaca tcatgttgac tgtttttggg aaaaagtttg 660attttgcaaa ggatgagggg ttggagcttg agttgatcct taaggaagga tatgagttac 720ttgggatctt caactggggt gatcatttgc ctcttttggg atggttagat ttgcaaggtg 780tgaggagaag atgcagaaca cttgtggcta aggtcaatgt atttgtgaag aagatcatag 840acgagcataa gaggagagcc aacggcgtag ggattgatga gggtgaaggt gaagattttg 900ttgatgtgct tcttggtttg gaggagaaag atagactctc agaatctgat atggtcgcag 960ttctttggga aatgatcttt agaggaactg atactgttgc catcctattg gaatggacgt 1020tggctagaat ggttcttcat cctgatattc aatcgaaggc acaagttgag attgattctg 1080tcgttgactc ttcaaggcca gtattggatt ctgatatcca acgacttcct tatctccaat 1140ctatagtaaa agaaaccctt cgaatgcatc ctcctgggcc tctattgtca tgggctcgcc 1200tagctatcca tgacgttcct gttgatggtc acatgattcc tgctgggacg

actgcaatgg 1260tgaacatgtg ggcaataaca catgacgaat gcaactgggc tgagcctaac aaattcaatc 1320ctgatcgatt catcgatgaa gatgtcaata ttcttggttc cgatttaagg ttggcaccct 1380ttggctccgg taaaagagtt tgccctggca aaacgatggc attggctgca gttcatcttt 1440ggttggctca gttgctgaaa agcttcaaat tgcttccttc gagaaatggt gtagatttgt 1500ctgagtgcct aaagatgtct ctcgagatga agaatccttt ggtatgtgtg gctgttccaa 1560ggttcgagta gtcctgctaa gatgacgtct agttataaga aatttgttct ttgcaaattg 1620tggccaacat aaatgatttc gtaagctagc aacttatgga taatgtcggt acatgttcgt 1680ttaaagtgtc aactttgttt ggttgaattt taaaatttga cattgtaata aagattctct 1740ggttctatgt aaatattgta attcagctta taatataaga aagaaatgaa tttgttgct 1799101611DNAGlycine max 10aagcactatc cctcccacca tgacaagcca cattgacgac aacctctgga taatagccct 60gacctcgaaa tgcacccaag aaaaccttgc atgggtcctt ttgatcatgg gctcactctg 120gttaaccatg actttctatt actggtcaca ccccggtggt cctgcctggg gcaagtacta 180cacctactct cccccccttt caatcattcc cggtcccaaa ggcttccctc ttattggaag 240catgggcctc atgacttccc tggcccatca ccgtatcgca gccgcggccg ccacatgcag 300agccaagcgc ctcatggcct ttagtctcgg cgacacacgt gtcatcgtca cgtgccaccc 360cgacgtggcc aaggagattc tcaacagctc cgtcttcgcc gatcgtcccg tcaaagaatc 420cgcatacagc ctcatgttta accgcgccat cggcttcgcc tcttacggag tttactggcg 480aagcctcagg agaatcgcct ctaatcacct cttctgcccc cgccagataa aagcctctga 540gctccaacgc tctcaaatcg ccgcccaaat ggttcacatc ctaaataaca agcgccaccg 600cagcttacgt gttcgccaag tgctgaaaaa ggcttcgctc agtaacatga tgtgctccgt 660gtttggacaa gagtataagc tgcacgaccc aaacagcgga atggaagacc ttggaatatt 720agtggaccaa ggttatgacc tgttgggcct gtttaattgg gccgaccacc ttccttttct 780tgcacatttc gacgcccaaa atatccggtt caggtgctcc aacctcgtcc ccatggtgaa 840ccgtttcgtc ggcacaatca tcgctgaaca ccgagctagt aaaaccgaaa ccaatcgtga 900ttttgttgac gtcttgctct ctctcccgga acctgatcaa ttatcagact ccgacatgat 960cgctgtactt tgggaaatga tattcagagg aacggacacg gtagcggttt tgatagagtg 1020gatactcgcg aggatggcgc ttcatcctca tgtgcagtcc aaagttcaag aggagctaga 1080tgcagttgtc ggaaaagcac gcgccgtcgc agaggatgac gtggcagtga tgacgtacct 1140accagcggtg gtgaaggagg tgctgcggct gcacccgccg ggcccacttc tatcatgggc 1200ccgcttgtcc atcaatgata cgaccattga tgggtatcac gtacctgcgg ggaccactgc 1260tatggtcaac acgtgggcta tttgcaggga cccacacgtg tggaaggacc cactcgaatt 1320tatgcccgag aggtttgtca ctgcgggtgg agatgccgaa ttttcgatac tcgggtcgga 1380tccaagactt gctccatttg ggtcgggtag gagagcgtgc ccagggaaga ctcttggatg 1440ggctacggtg aacttttggg tggcgtcgct cttgcatgag ttcgaatggg taccgtctga 1500tgagaagggt gttgatctga cggaggtgct gaagctctct agtgaaatgg ctaaccctct 1560caccgtcaaa gtgcgcccca ggcgtggata agagagagtt gaagctttta t 1611111885DNAGlycine max 11ggcactcact gaagcactat ccctcccacc atgacaagcc acattgacga caacctctgg 60ataatagccc tgacctcgaa atgcacccaa gaaaaccttg catgggtcct tttgatcatg 120ggctcactct ggttaaccat gactttctat tactggtcac accccggtgg tcctgcctgg 180ggcaagtact acacctactc tccccccctt tcaatcattc ccggtcccaa aggcttccct 240cttattggaa gcatgggcct catgatttcc ctggcccatc accgtatcgc agccgcggcc 300gccacatgca gagccaagcg cctcatggcc tttagtctcg gcgacacacg tgtcatcgtc 360acgtgccacc ccgacgtggc caaggagatt ctcaacagct ccgtcttcgc cgatcgtccc 420gtcaaagaat ccgcatacag cctcatgttt aaccgcgcca tcggcttcgc ctcttacgga 480gtttactggc gaagcctcag gagaatcgcc tctaatcact tcttctgccc ccgccagata 540aaagcctctg agctccaacg ctctcaaatc gccgcccaaa tggttcacat cctaaataac 600aagcgccacc gcagcttacg tgttcgccaa gtgctgaaaa aggcttcgct cagtaacatg 660atgtgctccg tgtttggaca agagtataag ctgcacgacc caaacagcgg aatggaagac 720cttggaatat tagtggacca aggttatgac ctgttgggcc tgtttaattg ggccgaccac 780cttccttttc ttgcacattt cgacgcccaa aatatccggt tcaggtgctc caacctcgtc 840cccatggtga accgtttcgt cggcacaatc atcgctgaac accgagctag taaaaccgaa 900accaatcgtg attttgttga cgtcttgctc tctctcccgg aacctgatca attatcagac 960tccgacatga tcgctgtact ttgggaaatg atattcagag gaacggacac ggtagcggtt 1020ttgatagagt ggatactcgc gaggatggcg cttcatcctc atgtgcagtc caaagttcaa 1080gaggagctag atgcagttgt cggaaaagca cgcgccgtcg cagaggatga cgtggcagtg 1140atgacgtacc taccagcggt ggtgaaggag gtgctgcggc tgcacccgcc gggcccactt 1200ctatcatggg cccgcttgtc catcaatgat acgaccattg atgggtatca cgtacctgcg 1260gggaccactg ctatggtcaa catgtgggct atttgcaggg acccacacgt gtggaaggac 1320ccactcgaat ttatgcccga gaggtttgtc actgcgggtg gagatgccga attttcgata 1380ctcgggtcgg atccaagact tgctccattt gggtcgggta ggagagcgtg cccagggaag 1440actcttggat gggctacggt gaacttttgg gtggcgtcgc tcttgcatga gttcgaatgg 1500gtaccgtctg atgagaaggg tgttgatctg acggaggtgc tgaagctctc tagtgaaatg 1560gctaaccctc tcaccgtcaa agtgcgcccc aggcgtggat aagagagagt tgaagctttt 1620attaaaaagg gaacaagaaa aaaagaaaat gaaatatata gaaataaaac agacaagaaa 1680gtaaagtaaa gattatgcat gttgctgcat gtaggttggt ggttggtggc aggtgtgcag 1740ccacacaatg gtaatatggt ggaagggatg ggttaggctc tctttttttt ttttagtggt 1800caagtattaa gtcttctcag cttgtcttct tattacaaaa aaagtactgt tgccagtgta 1860aataaactta atacgttttt agttg 1885121686DNAGlycine max 12atgtcaaccc acattgaaag cctgtgggtg ttggccttag cctcaaaatg cattcaagag 60aacattgcat ggtcactctt gatcatcatg gtcactctct ggctcaccat gaccttcttc 120tactggtctc accctggtgg tcctgcttgg ggcaaatact actactttaa ttactggaaa 180aaaaccacct caaccaacac aaacatcaac cttaaaatga ttatccctgg tcctagaggc 240taccctttca ttgggagtat gagtctcatg acatccctcg cacaccaccg tattgctgcg 300gcgggggaag catgcaacgc caccaggctc atggcttttt ccatgggtga cacacgcgcc 360atagtaacgt gcaaccccga tgtcgctaaa gagattctca atagttccac ttttgctgat 420cgtcccataa aggaatcagc ttacagcctc atgttcaacc gcgccatcgg cttcgcccct 480tacggcgtct actggcgtac cctccgccgc atcgccgcca cgcacctctt ctgccccaaa 540caaatcaaag cctccgagct ccagcgcgct gaaatcgccg cccaaatgac aaactcattc 600cgaaatcacc gttgcagcgg cggtttcgga atccgcagcg tgctcaagag agcgtcactg 660aacaacatga tgtggtcggt gtttggacaa aagtacaacc ttgacgagat aaacaccgca 720atggacgagc tatccatgtt ggtggaacaa ggctatgact tgttgggcac ccttaattgg 780ggagaccata tccctttcct gaaagacttt gacctacaga aaatccggtt cacctgctcc 840aaattagtcc ctcaagtgaa ccggttcgtt ggttcaatca tcgccgacca ccaggccgac 900acaacccaaa ccaaccgcga tttcgttcat gttttgctct ctctccaagg tcccgataaa 960ttgtctcact ccgacatgat tgctgtcctc tgggaaatga tatttagggg gaccgacacg 1020gtggcggttt tgattgagtg gatactggcg aggatggtgc ttcatccgga ggtgcaaagg 1080aaggtacaag aggagttgga cgcggtggtt aggggtggcg ctttgacgga ggaggtcgtg 1140gcggcgacgg cgtatcttgc ggcggtggtg aaagaggttc tgaggctgca cccgccgggc 1200ccgcttctct cgtgggcccg cttggccatc actgatacga ccattgatgg gtatcacgtg 1260cctgcgggga ccaccgctat ggttaatatg tgggccatag caagggaccc ggaggtgtgg 1320ctggacccac ttgagttcaa gcccgagagg ttcatgggtc tggaaaacga gttttctgtt 1380ttcgggtcgg atctgagact cgctccattc ggttcgggtc ggagaacatg ccccgggaag 1440actttgggtt tgagcaccgt aaccttctgg gtggcttggc ttttgcatga gtttgaatgg 1500ctaccgtctg atgaagccaa ggttgatcta acggaggtgc tgaggctctc gtgtgaaatg 1560gctaacccac tcattgttaa agttcgccct aggcatggat taagcactta atgataatat 1620aattaagcct atctacgtta ttaacttgaa atgttttaat gggaaggaaa aaaaaaaaaa 1680agagag 1686132100DNAOryza sativa 13aactcaaact gcacttccct tttcagctct tctccaacct caatttcacc ccatttttcc 60atatcctctc tgcacacgta cgtacaggta aacgtgcctg atcatggaca tggactcgtc 120gccgtcgaca caggactgtg gcggctggct gctgtacgtc tccctcgctg ccaaatgcgg 180cggcgaccct tgccgcgtcg tcggcttcgt cgccgttgcc gtcgtcgcct tcgccgtcac 240gtcgctcctg cactggctgt cgcccggtgg cccggcgtgg gggaggtatt ggtggaacag 300gaggggtggt ctgggcattg ctgccgccat tcctgggccc cgtgggttgc ccgtgctcgg 360cagcatgtcg ctcatggcgg gactcgcgca ccggaagctc gccgcggcgg cggggggctc 420gccggcgagg cggcgcctca tggcgctgtc tctcggggag acacgggtgg tggtcaccgc 480cgaccccggc gtcgcgcggg agctcctcgc cagcgcggcg ttcgccgacc ggccggtgaa 540ggagtccgcg tacgggatgc tgttccaccg cgccatcggg ttcgcgccct acggcacgta 600ctggcgcgcg ctccgccgcg tcgcgtccac gcacctcttc tcgccgaggc aggtgtccgc 660ctccgccgcg cagcgcgcgg tgatcgcgcg ccagatggtg gaggccatga ggtccgccgc 720cgccgccgcc gccggtggcg gcgtggcggc gaggccgttc ctgaagcgcg cgtcgctgca 780caacgtgatg tggtcggtgt tcgggaggaa gtacgagctg gcggcgccgg agagcgagga 840gacggcggag ctgaggagca tggtggacga aggctacgac ctcctcggcc agctcaactg 900gtccgaccac ctcccatggc tcgcaccctt tgacctcaag aagacgcggt caaggtgctc 960gtcccttgtc ccccgcgtca accgcttcgt cacccgcatc atcgacgagc accgtgctcg 1020cctcagcctc gccgtcgacg ccgccgtcga cttcaccgac gtccttctct ccctccacgg 1080cggcgacaag ctctccgacg ccgacatggt cgccgtcctc tgggagatga tctttcgagg 1140gacggacacg gtggcggtcc tgatcgagtg ggtggcggcg aggctggtgc tgcaccagga 1200cgtgcaggcc agggtccatg acgagctgga ccgagtggtc gggtcggacc gggcagtgac 1260cgagtcggac gcgtccaagc tggtctacct ccaagcggtg atcaaagagg tcctgcgcct 1320ccacccgccg ggcccactgc tctcgtgggc acgcctcgcc acgtcggatg tacacgtcgg 1380cgggttcctc ataccctctg ggaccaccgc catggtgaac atgtgggcca taacccatga 1440ccctgccgtt tggcccgacc cgaacgagtt caaaccagag aggttcgtcg cagggccctc 1500gtcggaccag gccacggagt ttccgataat ggggtcggat ctcaggctcg cgccgttcgg 1560gtcaggaagg cgaagctgcc ccggcaagtc gctcgccatc gccactgtcg gattctgggt 1620tgccacgttg ctacacgagt tcgattggct tcccttgtca gataagtcgc gcggcgtcga 1680tctgtcggag gtgctgaagc tgtcgtgcga gatggcaacc ccgctggagg caaggctaag 1740gccgcgacgc aaggtgtgat gacgtgtcac caccgtcacg tgggactaag acgaggagag 1800ggaagccgac ttccacttcc ttctagtgct tgttgagatg tgtaaatgtc cctaaatgta 1860aagtgttacg ctttgagtag aaatgcccct acgttgtagt gcgtagtatt gtacacttgt 1920agtatgtaat gcttgtattt ttgtgtgttt tgcacgtcct aagtagtgga gtagtagctg 1980ataatagtta gttaattact ctgctattta gtcatagtta actacctacc tgcaggtgat 2040gagagtgaca gttttttttt gtttaattaa ctgcaggtga tgagtgtaga atagctcggt 2100141861DNABrachypodium distachyon 14atggcgaccc ctgaggactg tggcagctgg ttgctgtacc tgtcgctggc cgccaaatgc 60ggcggcgacg gcgaccaccc gcgccgcctg gccgggctcc ttgccgtctg cgccgccgct 120ttcctcgtca cctgcctcct gcactggtgc ttccccggcg ggccggcgtg gggccgctgg 180tggtggacgc ggcggggcct gggccgcggg cccgtcgtcc ctgggccgag gggcctgccg 240gtgatcggca gcatgtggct catgactggc ctcgcccacc gcaagctcgc cgcggaggct 300gcccgcctgc gaggcggcgg gcgccggctg atggccttct ccctcggcga gacgcgcgtg 360gtcgtggcgg gccatcccga cgtggcccgg gagatcctga ccagcccggc cttcgccgac 420cggcccgtca aggagtccgc ctacgggctc atgttccacc gcgccatcgg cttcgcgcgc 480cacggcgcct actggcgcgc gctccgccgc gttgcttcca cgcacctctt ctcgccctgg 540caggtcgccg cgtccggcgc ccagcgcgcg gtgatcgcgc gccagatggt ggccgccctc 600gccgggggcg ccgaggtccg gcgcgtcctg cgtcgcgcgt cgctgcacaa cgtgatgtgg 660tcggtgttcg gccgccgcta cgacctggag ctggaccctg gcaaggaggt ccgcgagctg 720ggccagctcg tggacgaagg ctacgacctg ctgggccagc tcaactggtc cgaccacctc 780ccctggctcg cccgcttcga cctgcagggc acccgggccc ggtgcgccag cctagtgccc 840cgcgtgaacc gcttcgtcgg cggcatcatc gatgaccacc gggtcaaagc tccgtccgcc 900gtcaaggact tcacggacgt cctgctgggc ctgcaaggcg gcgacaggct cgccgactcc 960gacatggtcg cggtgctctg ggagatggtg ttccgtggca cggacacggt ggccgtgctg 1020atggagtggg tgctggcccg gctcgtgctg caccaggacg tgcaggcccg ggtgcacgag 1080gagctggacc gcgtcgtcgg gcgcgaccgg gccgtggccg agtccgacgc ggcctcgctc 1140gcctacctcc acgccgtggt caaggaggtc ctgcgcctcc acccgccagg cccgctgctg 1200tcctgggccc gcctggccac gtcggacgtg cacgtggacg ggttcctcat ccccgctggc 1260accaccgcca tggtgaacat gtgggccatc acccacgacg gcgacgtctg ggccgagccc 1320atggagttcc ggcccgagcg gttcgtcggg ccgggggctg aggagttctc cgtcatgggc 1380tctgatctcc ggctggcgcc gtttggggcc ggccggagga gctgccccgg gaagagcctg 1440gccatggcga ccgtggcgtt ctggctcgcc acgctgctcc acgagttcga cctgcttcct 1500tcctccgacc cggcacgtgg cgtgcaactg tcggagaccc tgaggctgtc gtgcgagatg 1560gccaccccgc tggccctgac gccgagggct cgtcgacgcc cggcggtttg aatttgaatt 1620gatgacgtat catgcctacg ccaccactgg ctagctagct agaaccctag tattgttgct 1680gctttgtttt tgagacgttg tcgcctaccg gtcggccgcc ggctcgctac cacgcacgca 1740cgtacgggca taacgctggc ttatctcgtc cagagctagc aaatgaatcg tttggacttt 1800tatatatcga gacgtgccag ctgatgacag cgatgttttt tgccttcttg ttcccccggt 1860c 1861151755DNAGlycine max 15atgacaaccc acattgataa cctgtgggtg ttggccttgg tctcaaaatg cacacaagag 60aacattgcat ggtcactctt gaccatcatg gtcactctct ggctctccat gaccttcttc 120tgctggtctc atcccggtgg tcctgcttgg ggcaagtact actcctttca ttactggaaa 180aaaacaacca caaccacaac ctcaacctca aacaacacaa actccaacaa ccttaaaatg 240attcccggtc ccaaaggcta tcctttcatt ggaagcatga gcctcatgac atcccttgca 300caccaccgta ttgctgccgc tgctcaagca tgcaaagcca ccaggctcat ggccttctcc 360atgggtgaca cgcgtgtcat cgtcacgtgc cacccccacg tggccaagga gattcttaac 420agctccgtct tcgccgatcg tcccataaag gaatcagcct acagcctcat gttcaaccgc 480gccatcggct ttgcccctta cggcgtttac tggcgcaccc tccgccgcat cgccgccacg 540cacctcttct gccccaaaca aatcaaggcc tcggagctcc agcgcgccga aatcgccgcc 600cagatgaccc actcgttccg aaaccgccgc ggcggtttcg gaatccgcag cgttctcaag 660agagcgtcgc tcaacaacat gatgtggtcg gtgtttggac aaagatatga ccttgacgag 720acaaacactt cagtggacga gttatcccgg ttagtggaac aaggctatga cttgttgggt 780acccttaatt ggggagacca tatccctttt ctgaaagact ttgaccttca aaaaatccgg 840tttacctgct ccaaactcgt cccccaagtg aaccggttcg taggttcaat catcgccgac 900caccaaaccg acacaaccca aaccaaccgc gatttcgttc atgttttgct ctctctccaa 960ggtcccgata aattgtctca ctccgacatg attgctgtcc tctgggaaat gatatttagg 1020gggaccgaca cggtggcggt tttgattgag tggattatgg caaggatggt gcttcatccg 1080gaggtacaaa ggagggtgca agaggagctg gacgcggtgg ttggaggtgg tgcgcgcgct 1140ttgaaggagg aggacgtggc ggcgacggcg tatcttctgg cggtggtgaa ggaggttctg 1200aggctgcacc ctccaggccc gcttctctcg tgggcccgct tggccatcac cgatacgacc 1260attgatgggt ataacgtgcc cgcgggaacc accgccatgg ttaatatgtg ggccatagga 1320agggacccgg aggtgtggct ggacccactt gatttcaagc ccgagaggtt catgggcctg 1380gaggcggagt tttctgttct cgggtcggat ctgaggctgg ctccattcgg gtcgggtaga 1440agaacctgcc ccggaaagac tttgggtttg agcaccgtga ctttctgggt ggcgaggctt 1500ttgcacgagt ttgaatggct accatctgat gaggggaagg ttgatctaac ggaggtgctg 1560aggctctcgt gtgaaatggc taacccgctc tatgttaaag ttcgccctag gcgtggatta 1620agtacttaat aataataata ataataataa taataataat aataatgtta agtagcaggt 1680gcatggccct ttggagccac taaatgttaa gtgaatccat gaatcaaggt agaaagtttg 1740agttggctct gtctc 1755161662DNAOryza sativa 16atggcgactc cggaggacac tggcagctgg ctgctctacc tctccttggc ggctaaatgc 60tccggcgatg gcgacggcca gcctcaccgg cttcttgggt tcgtcgtggt ttgcgccgtc 120gctggtctgg ttacatgtct gctgcactgg tccttccccg gagggccggc gtgggggagg 180tggtggtgga cgcggcggcg gcgtcggggg tcgccgtgcg gtgtggcggc tgttcctggg 240ctgagggggc tgccggtgat cggcagcatg tggctcatga ccgggctggc gcaccggaag 300ctcgccgcgg cggcggaggc ggcgggggcg gggcggctga tggcgctgtc gctcggggag 360acgcgggtgg tcgtggcggc gcacccggac gtggcgaggg agatcctgca cggcgcggcg 420ttcgccgacc gccccgtgaa ggagtccgcg tacgggctgc tgttccaccg cgccatcggg 480ttcgcgcccc acggcgcgta ctggcgcgcg ctgcggcggg tggcgtccac gcacctcttc 540tccccgtggc aggtcgcggc gtccgcgccc cagcgcgcgg tcatcgcgcg ccagatggtc 600cgcgccatca agctgcagca gcggagccgg agcggcgatt ccgccgccgg cgccgccgtc 660gaggtccgcc gcgtcctgcg ccgcgcgtcg ctccacaacg tgatgtggtc ggtgttcggc 720cggcggtacg agctgcagct ggaccccggc aaggagagcg acgaggtccg ggagctgagg 780gccctcgtcg acgaaggcta cgacctgctc ggccagctca actggtccga ccacctccca 840tggctcgccc gcttcgacct gcagagcacc cgcgcccgct gctcccgcct cgtcccccgc 900gtcaaccgct tcgtcacccg catcatcgac gagcatcgct catctgctcc cgtcgcagcc 960gccatcgact tcaccgacgt cttgctctcc ctgcagggca gcgacaagct cgccgactcc 1020gacatggtcg ccgttctctg ggagatggtg tttcgcggga cggacacggt ggccgtgctg 1080atcgagtggg tcttagcccg gctcgtgctg cagcaggacg tgcaggctcg ggtgcacgac 1140gagctgggcc gggtggttgg gctggaccgg gacgtgaccg agtccgacac ggcctcactc 1200gtctacctcc acgccgtcat caaggagacg ctgaggctgc acccaccggg cccactcctc 1260tcatgggccc gcctggccac gtcggacgta cacgtggacg ggtacctgat ccccgctggc 1320accaccgcga tggtgaacat gtgggccata gcacacgacc ccgacgtgtg ggccgagccg 1380atggagtttc ggcccgagcg gttcatcggg aaggcggcgg agttcagtgt aatgggttcg 1440gatctcaggc tcgcgccgtt cggatcgggt cggcggagct gccccgggaa gagcctcgcc 1500atggccacgg tggcattctg gcttgccacg ctgttgcacg agttcgccct cctcccctcg 1560cccgacccgg cacacggcgt cgacttgtcg gaggtgctaa ggctgtcgtg cgagatggcc 1620accccgctgg cggtgacagc gtggcctcgg cgtgtggtgt ga 1662171672DNAGlycine max 17acaccatcca ctactcttct tagttccagc acaacaagct cttcatttct cccacacttt 60cttttctttc accaaaaatg tcaccagatt tcacactttt gttcttcccg gaactcatgc 120agtcccctat gatcactttc caagccaccc tctgcgtcct tctcttcacc ctcatgttca 180cgctgctctt cactcctggt gggcttcctt gggcctgggc ccggcccaga cccatcatcc 240ctggcccagt aactgccctg ttagggatct ttactggctc cacgcctcac cgtgctttat 300ccaaactcgc ccgtaattac cacgcggaaa aactcatggc tttctccatc ggtttaaccc 360gtttcgtcat ctccagcgaa ccggagaccg ctaaggagat tctcggcagc cccagtttcg 420ctgataggcc ggtgaaggaa tccgcctatg agcttctctt ccaccgcgca atgggttttg 480caccgtatgg ggagtactgg aggaatttga ggagaatctc agccctacat ctcttctccc 540cgaagagaat caccggctct gaatccttca ggagcgaggt tggattaaaa atggttgaac 600aagttaagaa aaccatgagt gagaaccaac atgttgaggt taagaaaatt ctacacttta 660gttcgttgaa caatgtgatg atgacggtgt ttggtaagtc ttatgagttt tacgagggtg 720agggtttgga gcttgagggt ttggtgagtg aagggtatga gttgttgggt gtttttaact 780ggagtgacca ttttccggtt ttggggtggt tggatttgca gggtgtgagg aagaggtgta 840ggtgtttggt tgaaaaggtt aatgtttttg ttggaggggt tattaaggag catagggtga 900agagggagag gggtgagtgt gtgaaggatg aaggaactgg ggattttgtt gatgttttgc 960ttgatttgga gaaggaaaac aggctcagtg aagctgacat gatcgctgtt ctttgggaaa 1020tgatatttag gggaactgac acggtggcaa ttctgctaga gtggactctg gctcggatgg 1080ttctccaccc tgaaatccaa gcaaaggcac agcgcgaaat agacttcgtt tgcggatcct 1140ccaggcccgt atccgaagca gacattccga acctgcgcta ccttcagtgc atagtaaaag 1200aaacccttcg tgtgcaccca ccaggcccgc tactctcgtg ggctcgcctt gctgtgcacg 1260acgttaccgt gggcggcaag cacgtgattc ccaagggcac caccgcgatg gtgaacatgt 1320gggccataac ccacgacgag agggtgtggg ccgagcccga gaagtttagg cccgagcggt 1380ttgtggagga ggatgtgagc ataatggggt ctgatttgag gttggcacct ttcgggtctg 1440gaagaagagt gtgccctggg aaggcccttg gtttggcctc ggttcatctt tggctcgctc 1500agttgcttca aaattttcat tgggtttcat ctgatggtgt

ttctgtggag ttggatgagt 1560ttcttaagct ttctatggag atgaagaagc cactgtcttg caaggctgtg cctagggttt 1620ctgtttaggt ttatgtgtgt tgttgggttg agttggtttg gtttgtctgc tt 1672181635DNAGlycine max 18tcagcacaac ctcttcatct ctcccaggct ttcttttctt tgcaacaaca ctaccaaaaa 60tgtcaccaga tttcacactt ttgttctccc cggaactcat gcagtcccct atcatcactt 120tccaagccac tttctgtgtc cttctcttca ccctcatgtt cacgccgttc ttcactcctg 180gtgggcttcc ttgggcctgg gcccggccca gaaccatcat ccctggccca gtaactgccc 240tgctcggggt cttcacgggt tccacacctc acagcgcttt atccaaactg gcccgcactt 300atcacgcgga aaagctcatg gctttctcca tcggtttaac ccggttcgtt atctccagcg 360aaccggaaac cgcaaaggag attctcggca gccccggttt tgcggacagg ccggtgaagg 420aatccgccta tgagcttctc ttccaccgtg caatgggttt cgcaccgtac ggagagtact 480ggagaaacct gaggagaatc tcagccctac atctcttctc cccgaagaga atcaccagct 540ctgagtcctt caggagcaag gttgggttaa aaatggttga acaagttaag aaaaccatga 600gtgagaacca acacgtcgag gttaagaaaa ttctacactt tagttcgttg aacaatgtga 660tgatgacggt gtttggtaag tgttatgagt tttacgaggg tgagggtttg gagcttgagg 720gtttggtgag tgaagggtat gagttgttgg gtgtttttaa ctggagtgac cattttccgg 780ttttggggtg gttggatttg cagggggtga ggaagaggtg taggtgtttg gttgaaaagg 840ttaatgtttt tgttggaggg gttattaagg agcatagggt gaagagggag aggggtgact 900gtgtgaagga tgaaggagct gaggattttg ttgatgtttt gcttgatttg gagaaggaaa 960acaggctcag tgaagctgac atgattgctg ttctttggga aatgatattc aggggaactg 1020acacggtagc aattctgcta gagtggattc tggctcgcat ggttctccac cctgaaatcc 1080aagcaaaggc acagcgcgaa atagacttcg tttgcggatc ctccaggctc gtatccgaag 1140cagacattcc gaacctgcgc taccttcagt gcatagtaaa agaaaccctc cgtgtgcacc 1200caccaggccc gctactctcg tgggctcgcc ttgctgtgca cgacgttacg gttggcggca 1260agcacgtgat tcccaagggc accaccgcga tggtgaacat gtgggccata acccacgacg 1320agagggtgtg ggccgagccc gagaagttta ggcccgagcg gtttgtggaa gaggatgtga 1380gcataatggg gtctgatttg aggttggcac ctttcgggtc tggaagaaga gtgtgtcctg 1440ggaaggccct tggtttggcc tcggttcatc tttggctcgc tcagttgctt caaaattttc 1500attgggtttc atctgatggt gtttctgtgg aattggatga gtttctcaag ctttctatgg 1560agatgaagaa gccactgtct tgcaaggctg tgcctagggt ttctgtttag gtttatgtgt 1620gttgttgggt tgagg 1635192158DNAZea mays 19cccgaatcgg gagtagtagt agtagtgcat ccaatcgaca cacacacaaa aaaaaaggtt 60agttagccat ggcgccgcca acagaggact gcggctggct gctgtacctt tccctggccg 120ccaaatgcgg cgaccctcag cgcctgctcg gcttcgccgc ggtcttcgtc gcggcctgcg 180tagtcacgtc gctcctgcac tgggcgtccc cgggcggccc cgcctggggg tggtactggt 240ggaccaggcg ggccgggctg ggcatcgtcc gtgccgccat cccgggaccc cggggcctgc 300cggtggtcgg cagcatgggc ctgatgaccg gcctggcgca ccgcaagctc tcggcggcgg 360ccgagagaca ggccagcagg cgccgcctca tggcgttctc gcttggcgag acccgggtgg 420tggtcaccgc cgaccccgac gtcgcgcggg agctgctcgc cagcgccgcc ttcgccgacc 480gccccgtcaa ggagtccgcg tacgggctcc tgttccaccg cgccatcggc tttgccccgc 540acggcgccta ctggcgcgcg ctccggcgcg tcgcgtccgc gcacctcttc tcgccgcgcc 600agatcgcggc ctccgcggcg cagcgcgcgg ccatcgcgcg ccagatggtg gacgccacga 660cgaccgccgc ggcccacgcc cccgtcgtcg tggcgcgccg gttcctgaag cgcgcgtcgc 720tgcacaacgt catgtggtcg gtgttcggcc gcaggtacga cctgatggcg gacagccggg 780aggccgagga gctcaaggcc ctggtagacg aaggctacga cctgcttggg cagctcaact 840ggtccgacca cctcccgtgg ctcgcccgct tcgacctgca gaagacccgg gccaggtgct 900gcgcgctcgt cccgcgggtg aaccgcttcg ttggcaacat catcggcgag caccgtgccc 960gcctcggccg cggcgtcgac accgccgtca tggacttcac ggacgtcctg ctctccctcc 1020agggcgacga caagctctcc gacgccgaca tgatcgccgt tctgtgggag atgatcttcc 1080gaggcacgga cacggtggca gtcctgatcg agtgggtgct ggcccgtctg gtgctgcacc 1140aggacgtgca gagcaaggtc caggaggagc tggaccgggt ggtcgggctg ggccaggccg 1200tgacggagtc ggacacggcc tcgctgccct acctccaggc ggtcatcaag gaagtgctac 1260gcctgcaccc gccaggccca ctgctctcct gggcgcgcct cgccacctca gacgtgcacg 1320taggcgggta ccttgtgccc gcgggcacca ccgccatggt gaacatgtgg gccataaccc 1380atgaccccag cctgtggcct gagccaatgg agttcaggcc cgagaggttc atgggccctg 1440ccgccgagga cgtcccgata atgggttcgg atctccggct cgcgcctttc gggtccggca 1500ggcggagctg ccccggcaag tcactcgcgg tggctaccgt cggattctgg gtcgccaccc 1560tgctgtacga gttcaaatgg ctgccgccgt ccgacgagcc acgcggcggc ggcgtcgacc 1620tatccgaagt gctgaggctg tcgtgtgaga tggctgcacc gctggaggcg agggtggtgc 1680cacgtcacgc ggtgtgctga gggggctgag acacgtggcc tgcaggggtg gggatcagag 1740gaggaaagct cgaccgatcg tcttctagct tctactacta cgtaatacct taccttgtag 1800cagaacgtaa cgtggtcgat atgagatgtg taaagaaaga aaaaaaaaga acgcccagtt 1860gcaccatgca tgctagctgc tggtgtggag tcagtacgta gtagcagcac ccggtttgat 1920cgatgcttat gtgtgtaatg taatacctac ctgcagttgc aagtaaatgt gtgcctgtta 1980ttagtctagc taggtagtgt agtgtagtgt accggatggt gagcgagcgt gacagttacc 2040tttccttttc agtactgcct agctagctag acctagatgt attattatat attgtgtact 2100cccttcttac gtacgtgcgt tccataataa atgtattctg tagttgtctt tttctact 2158202176DNASorghum bicolor 20cactcaaccc gagcgccact tctccaaagc atcagcgaac gagcgagctg agggagcgag 60agagagaaaa ggatagtgaa acacgagaga gctaggagcc atggcggtgg cggcgacccc 120cgatgactgc ggcagctggt tgctgtacct gtccctagcc gccaaatgcg ccggcggcga 180ccagcctcac cgcctggccg gtttcctggc ggtctgcgcc gtggccttcg tcgtcacttg 240cctcctccac tggtgcttcc ctggaggtcc ggcgtgggga aggtggtggt ggacgacgca 300ggcgcggcgg gtcgcggcgg ccgccgttcc ggggccgagg ggcctgcccg tggtcgggag 360catgtggctc atgacgggcc tggcgcaccg caagctggcg gcggcggcgg acagcctccg 420cgccaggcgc ctgatggcgt tctccctcgg cggcacgcgg gtggtggtgg ccgcgcaccc 480ggacgtggcg cgggagatcc tcaacagccc ggcgttcgcg gaccgtccca tcaaggagtc 540cgcgtacggg ctcctgttcc accgcgccat cggcttcgcg ccctacggcg cctactggcg 600cgcgctccgc cgcgtggcgt ccacgcacct cttctccccg tggcaggtcg ccgcctccgc 660cgcgcagcgc gccgtcatcg cgcgccagat ggtcgccgcc atgaagcagg agctgtcgtc 720gtcgtcgtcg gcctcggccg gcttcgaggt ccgccgcgtc ctgcgccgcg ggtccctgca 780caacgtgatg tggtcggtgt tcggccggcg gtacgacctg gagctggacc cggccaagga 840gagccccgag acgcgggagc tgaggagcct cgtggacgaa ggctacgacc tgctgggcca 900gctcaactgg tccgaccacc tcccctggct cgcgcgcttc gacctgcaga gcaccaggtc 960caggtgcgac cgcctcgtcc cgctcgtgaa ccgcttcgtc ggcggcatca tcgacgcgca 1020ccgcgcccgg aacgacctcc gctccgctcc accacacgcc gtcatggact tcaccgacgt 1080gctcctctcg ctgccggccg acgacaggct caccgactct gacatgatcg ccgtcctctg 1140ggaaatggtg ttccgtggaa ctgacaccgt tgccgtgctg atcgagtggg tgctggcgag 1200gctcgtgctg caccctgacg tgcaggcccg tgtccacgac gagctggacc gcgtggtcgg 1260gcgtgaccgg gccgtgaccg agtccgactc ggggtcactg gtctacctgc acgccgtgat 1320caaggaggtg ctcaggatgc acccgccggg cccactgctg tcgtgggcgc gcctggccac 1380gtcggacgtg caggtggacg ggcacctcat ccccgccggc accaccgcca tggtgaacat 1440gtgggccata acgcacgacc cggacgtgtg ggcggagccg gcggagttcc agccggagag 1500gttcatggga tccaccaccg gcggcgagtt cccgataatg gggtcggacc tgaggctcgc 1560gccgttcggg gcgggccggc gcagctgccc cgggaagagc ctcgccatgg ccaccgtggc 1620tctctggctc gcgacgctgc tgcacgagtt cgagctgctc ccggcgcgcg gcgtctacct 1680gtcggaggtg ctcaagctgt cgtgcgagat ggccgtcccg ctggccgtga cggcgaggcc 1740ccggcaagcg gtgtgatgac gcgtcacggc ggctgggacg acggagcagg caggcaggca 1800gtcagttggg gtatcagtct cactgtgagc attataccac aactactagt agtactacta 1860ctgtacacgg aatggaaaag cgcttgtgct tttgggagac gttgctaccg gtcacagctt 1920gcaagttgct actactgggt cgacatgggg tatatgcttt tcatgttact atcttcgata 1980tgtatcgaga tcaggttgcc gaatgtgata ctttggcttg tactgttagc ttttgtctgg 2040gtgctctttt attgcttttt ttttaagtag taatcgctgt aagactcgta aaatgtatat 2100gctggtttgg atggttttgg attgtagctc acaaactagt attacgcagt tcaatgcctt 2160aatatgctat ctgttc 2176211587DNAGlycine max 21atgtcatcat cggaactctc ctctttcttt cttctccgtc tatcagacat actcagtttc 60gatgttttgc tcggagttat gtttctagtt gccgtgttcg gctactggct ggttcccggc 120ggtcttgctt gggctttttc caagttcaag cccgcgattc ccgggccttc cggttacccg 180gtggtgggtc tggtttgggc tttcataggg cctcttactc acagggtcct tgctaagttg 240gctgaaacct ttgatgcaaa gcccttgatg gcgttctctg tggggttcac tcgtttcatc 300atctcttctc accccgacac cgccaaagag atcttgaaca gttccgcttt cgcggatcgt 360cccgttaagg aatctgctta tgaacttctc tttcaccgtg cgatggggtt tgcaccctac 420ggtgagtact ggaggaacct gaggaggatc tccgcaactc acatgttctc tccgaggaga 480atcgcagccc aaggagtgtt tcgggcccgg attggagccc aaatggtgag agacatcgtg 540ggcctgatgg ggagggacgg tgtggtggag gtgaggaagg tgttgcattt tgggtcgttg 600aataacgtga tgaagagcgt gtttgggagg agttatgtgt ttggtgaggg gggtgatggg 660tgtgagctcg aggggttggt gagcgagggg tatcatttgc ttggggtgtt taactggagt 720gaccactttc cactcttggg ttggttggat ttgcaaggtg tgaggaagag ctgtaggagt 780ttggttgata gagtgaatgt ttatgttggg aaaatcattt tggagcatag agtgaagagg 840gttgctcaag gtgaggataa taaggccatt gatactgata gttctggtga ctttgttgat 900gtgctgctgg atttggagaa agagaatagg ctaaaccact ctgatatggt tgctgttttg 960tgggaaatga tatttagagg gactgatacg gtagctatcc ttctagagtg gattctagca 1020agaatggttc ttcatccaga aatacaagca aaggcacaat ctgaaataga ctctgtggtt 1080gggtctgggc gtagtgtgag tgatgatgac cttccaaacc ttccttacgt tcgagccata 1140gtgaaggaaa ccttaaggat gcacccacca ggccctcttc tttcatgggc cagactttct 1200attcatgaca cacaaattgg caatcacttt gttccagctg gcaccactgc tatggtaaac 1260atgtgggcca taactcacga ccaagaagtg tggtatgagc caaaacagtt caagccggag 1320cgttttttga aggacgagga cgtgccaatc atgggatctg atcttaggtt ggcacctttt 1380ggctctggga ggagagtgtg ccctggaaaa gccatgggct tggccactgt tgagctttgg 1440cttgctatgt tcttacaaaa attcaaatgg atgccctgtg atgattctgg tgttgacttg 1500tctgagtgtt tgaagctctc catggagatg aaacactctc tcaaaaccaa agttgttgca 1560aggcctgtag tttctcttgc aatgtaa 1587222074DNAZea mays 22aaagacttga accacgcaac cctctctggt ctctggtcgt cctactttct tccacagcag 60aaggaaactt gagtcgtgag cgcagtggtg catccaatcc acaaagagct gaggttagtc 120agccatggcg gcaccgccga ccgaggactg cggctggctg ctgtacctct ccctggccgc 180caaatgcggc gaccctagcc gcctgctcgg cttggcggcg gtcttcgtcg gcgcctgcgt 240cgtaacgtcg ctcctgcact gggcgtgccc gggcggcccc gcctgggggc ggtactggtg 300gaccaggcga ggcgggctgg gcatcgtccg cgccgccatc ccgggacccc ggggcctgcc 360agtggtcggc agcatggggc tgatgaccgg cctggcgcac cgcaagctcg cggcggcggc 420ggcggcggcc gggggtcagg gcagcagcag gcgccgccgt ctcatggcgc tctccctcgg 480cgagacccgg gcggtggtga ccggcgaccc ggacgtcgcg agggagctgc tcggcagcgc 540cgccttcgcc gaccgccccg tcaaggagtc cgcgtacggg ctcctcttcc accgcgccat 600cggcttcgcc ccgcacggcg cctactggcg cgcgctccgc cgcgtggcgt cggcgcacct 660cttctcgccg cgccaggtcg cggcctcctc cgcgcagcgc gcggtcatcg cgcgccagat 720ggtggacgcc gtgaccacgg ccgcccccgc ccccgccccc gccgtcgtgg tggcgcgccg 780gttcctgaag cgcgcgtcgc tgcacaacgt catgtggtcg gtgttcgggc gcaggtacga 840cctgctgctg ctggcggcgg acggcgagga gctgaaggcg ctggtggacg aaggctacga 900cctcctcggg cagctgaact ggtccgacca cctcccgtgg ctggcccgct tcgacctgca 960gaggacccgg gccaggtgct ccgcgctcgt cccgcgggtg aaccgcttcg ttggcaacat 1020catcgacgag caccgtgcgc gcctcggcct cggcgacacc ggcggcgtca cggacttcac 1080cgacgtcctg ctctccctcc agggcgtcga caagctctcc gacgccgaca tggtcgccgt 1140tctctgggag atgatcttcc gaggcacgga cacggtggcc gtcctaatgg agtgggtgct 1200ggcgcgtctc gtgctgcacc aggacgtgca gagcaaggtc caggaggagc tggaccgggt 1260ggtggggcca ccgggccagg ccgcatccgt gacggagtcg gacaccgcct cgctcgtcta 1320cctccaggcg gtcatcaagg aagtgctgcg cctgcacccg ccaggcccgc tgctctcctg 1380ggcgcgcctg gccacgtcgg acgcgcgcgt aggcgggtac cacgtgcccg cgggcaccac 1440cgccatggtg aacatgtggg ccataacgca tgaccccagc gtgtgggccg agccgacgga 1500gttcaggccc gagaggttcg tgggcgcctc tgctggtgct ggtgctggtg ctggtgccga 1560ggacgttccg atgataatgg gctcggatct ccggctcgcg cccttcgggt ccggcaggcg 1620gagctgcccc ggcaagtcgc tcgcgctggc taccgtcggg ttctgggtgg ccaccctgct 1680ccacgagttc aaatggttgc cgccgtgccg cggcgtcgac ctgtccgagg tgctgaggct 1740gtcgtgtgag atggctgcac cgctggaggc gagggtggtt ccacgtcacg cggtgtgaga 1800tgacgaggat gagacacgtg gcctggggat aggagaagtt gcccgatcgt ctgtctagat 1860tgtactgtac tatatatctt atcttagctt tccttacctt gtagcagaga acgtaacgtg 1920gcccggcggc gatggatgat gagaatgaga tgtgtaaaag aagaaaaaga gcatgcccga 1980gtgccatatg ctttagagtg gactgtgtat atatgatgat gctattgcta aaaaaaaaaa 2040aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 2074232061DNABrachypodium distachyon 23atggcgccgg cgacttctgc ttccgaggac tgcgcggggt ggctgctgta cgcgtccctg 60gccgcgagat gcaacgacgg cggtgaggcc taccgggccg ccgtcttcgc catggccctg 120ctggccacca gtttcatcct cacctcgctc ctccactggg cctccacccc cggcggcccg 180gcctggggac gctaccgctg gacttccacc acctctcggg ccgccattag cactagccct 240cgcatccccg gcccgcgcgg gctgccggtg gtcggcagca tgggcctcat gacgggcctg 300gcccaccgca agctcgccgc ggctgtcgcc gccgggggag acgacgaaga ggagaggtcc 360cagcggaggc ggctgatggc gttctcgatg ggcgagacgc gggccgtggt gagctcggac 420ccggccgtgg cccgcgagct gctgtcgagc ccggcgttcg cggaccggcc cgtcaaggag 480tccgcctacg ggctcctctt ccaccgcgcc atcggcttcg cgccccacgg cgcctactgg 540cgctccctcc gccgcgtcgc ctcggcgcac ctcttctcgc cgcgccaggt cgccgcctcc 600gccgcccacc gcgccgccat cgcccgcagc atggttggct ccgtctccgc catcgccatg 660ggctccggcg aggtcgaggt ccgccggttc ctgaagcggg cggcgctgca cggggtcatg 720tggtccgtgt tcggccggag gtacgacggc acggcggcgc cggagctggg gaagaaggag 780gaggaagagc tgaggagcat ggtggaagaa ggatacgagc tcctcggcaa gctcaactgg 840gccgaccacc tgccatggct ggcccgcttc gacctccagg ggatacgggc ccggtgcgcc 900gccctcgtgc cacgcgtcaa ccgcttcgtc ggcaagatcg tcgacgacca ccgcgccgct 960gctgccgccg acgccggcga tcgtgtcgtg gacttcaccg acgttctgct atcccttcaa 1020ggcgccgaca agctctccga cgccgacatg atcgcggttc tctgggagat ggtgttccgt 1080ggcacggaca cgatggcggt ggtgatggag tgggtgctgg cccggctggt gatgcaccag 1140gacgtgcagg ccagggtcca ggaggagctg gaccgggtgg tcgggccggg ccaagccgtc 1200tccgaatcgg acgcggcccg gctcgtctac ctccaggccg ttattaagga gacgatgcgg 1260ctgcacccgc caggccccct gctctcatgg gcccggctcg ccacatcgga cgttcatgtg 1320ggcgggttcc tcgtgccagc tggcaccacc gccatggtta acatgtgggc catcacccat 1380gacccgaccg tgtgggcgga tccgctggag ttcaaccccg acaggttcat tgtcggagcc 1440gttccgttgt cggaaggtca tcataatgcc gttccgggcg ctgagttctc cataatgggc 1500tcggatctca ggctcgcgcc attcggatcg ggcaggcgga tctgccccgg gaagccactg 1560gcgatggcca gcatcgggtt ttgggtcgcg acgctcctcc atgagttcaa gtggacctcg 1620gcgccacgtg gtgacgtcga cctgtcggag gtgctgaggc tgtcatgcga gatggccgcc 1680ccgctcaagg cgaggctcac accaaggcgc cctgtgtgat gatatgccgg agccaccgac 1740cacttcttca ccggactagt cctgtctatc ttctttgctt attttctctc taccgatgcc 1800tgtagtgaaa aaaaagaagt aatgtcccgg tttggaaacg tgcctgctgc tataataggt 1860ccggtcctgg tcgtacttct ctagtccttt tgctgtactt agcttagcta aaagagatgc 1920taaattaggt acttatgaac tactagtatt ataaggaaat gagtgtgaca gttttcttgt 1980gtgttttccc tttgctgcct agctagaggt ttctatgcat gtaaatacct ttatatttcc 2040caatgcaagc ctttgtttgc t 2061241716DNAGlycine max 24acaccacagt ttcaaagccc ttctaaaacg ccttactctg tatcttcttt catcatccaa 60aaaaaaccat tcacacaaag aaaacctttg ggtttcccaa gatgtcatca tcggaactct 120cttctttctt tctcctgccc ctatcagcca tactcagttt cgatgctttg ctcggagtta 180tgtttctagt ggccgtgttc ggctactggc tggttcccgg tggtcttgct tgggctttgt 240ccaaattcaa gcctgcgatt cccgggcctt gcggttaccc ggtggtgggc ctggtttggg 300ctttcatagg gcctcttact cacagggtcc ttgcaaagtt ggctgaaacc ttcgatgcaa 360agcccctgat ggcattctcg gtagggttta ctcgctttat catctcttct caccccgaca 420ctgccaaaga gatcttgaac agttctgctt ttgcggatcg tcctgttaag gaatccgctt 480atgagcttct ctttcaccgc gcaatggggt tcgcacccta cggtgagtac tggaggaatc 540tgaggaggat ttcagcgact cacatgttct ctccgaagag aattgcggcc caaggagtgt 600tccgggcccg ggttggggcc caaatggtga gagaaatcgt gggcctgatg gggaagaatg 660atgtcgtgga ggtgagaaag gtgttgcatt ttggatcgtt gaataacgtg atgaagagtg 720tgtttgggag gagctatgtg tttggtgagg ggggtgatgg gtgtgagctt gaggagttgg 780tgagtgaggg gtatgatttg cttgggctgt ttaactggag tgaccacttt cctctcttgg 840gttggttgga ttttcaagga gtgaggaaga ggtgcaggag tctggtggat agagtgaatg 900tttttgttgg gaaaatcatt atggagcata gagtgaagag ggatgctgaa agtggtgact 960ttgttgatgt gctgttggat ttggagaaag aggataggct aaaccactct gatatggttg 1020ctgttttgtg ggaaatgata tttaggggga ctgatacagt ggcaattctt ctagagtgga 1080ttctagcaag gatggtactg catccagaaa tacaagcaaa ggctcagtgt gaaatagact 1140ctgtggttgg gtctgggtgc agtgtgactg atgatgacct tcctaacctc ccttacgtgc 1200gagctatagt gaaggaaacc cttaggatgc acccaccggg ccctcttctt tcatgggcca 1260ggctttccat tcacgagaca caaattggca accactttgt tccagctggc acaactgcta 1320tggtcaactt gtgggccatc actcatgacc aacaagtgtg gtccgagcca gaacaattca 1380agcccgagcg gtttctgaag gacgaggacg tgccaatcat ggggtctgat cttaggttgg 1440caccttttgg cgctggtagg agagtgtgcc ctggaaaagc catgggcttg gccactgttg 1500agctttggct tgctgtgttc cttcaaaagt tcaaatggat gccttgtgat gattctggtg 1560tggacttgtc tgagtgcttg aagctctcca tggagatgaa acactccctc atcaccaaag 1620ctgttgcaag gcctacatct tctcttgcaa tgtaatgggt tggagtatcc catcatttta 1680ctccccttaa taataatatt tctttccttt taaaaa 1716251578DNAOryza sativa 25atggcgctct cctccatggc cgcggcgcaa gagagctccc tcctcctctt cctcctcccg 60acgtcggccg cctccgtgtt cccgccgctc atctccgtgg tcgtcctcgc cgcgctcctc 120ctgtggctct cgccgggtgg ccccgcgtgg gcgctgtccc gttgccgtgg cacgccgccg 180ccgccgggcg tggcgggggg cgcggccagc gcgctgtccg gccctgccgc gcaccgcgtg 240ctcgccggga tttcgcgcgc cgtcgagggc ggcgcggcgg tgatgtcgct ctccgtcggc 300ctcacccgcc tcgtcgtggc gagccggccg gagacggcga gggagatcct cgtcagcccg 360gcgttcggcg accgccccgt gaaggacgcg gcgaggcagc tgctgttcca ccgcgccatg 420gggttcgccc cgtcgggcga cgcgcactgg cgcgggctcc gccgcgcctc cgcggcgcac 480ctcttcggcc cgcgccgcgt ggccgggtcc gcgcccgagc gcgaggccat cggcgcccgc 540atagtcggcg acgtcgcctc cctcatgtcc cgccgcggcg aggtccccct ccgccgcgtc 600cttcacgccg cgtcgctcgg ccacgtcatg gcgaccgtct tcggcaagcg gcacggcgac 660atctcgatcc aggacggcga gctcctggag gagatggtca ccgaagggta cgacctcctc 720ggcaagttca actgggccga ccacctgcca ttgctcaggt ggctcgacct ccagggcatc 780cgccgccggt gcaacaggct agtccagaag gtggaggtgt tcgtcggaaa gatcatacag 840gagcacaagg cgaagcgagc tgccggaggc gtcgccgtcg ccgacggcgt cttgggcgac 900ttcgtcgacg tcctcctcga cctccaggga gaggagaaga tgtcagactc cgacatgatc 960gctgttcttt gggagatgat ctttagaggg acggacacgg tggcgatctt gatggagtgg 1020gtgatggcga ggatggtgat gcacccggag atccaggcga aggcgcaggc ggaggtggac 1080gccgccgtgg ggggacgccg cggccgcgtc gccgacggcg

acgtggcgag cctcccctac 1140atccagtcca tcgtgaagga gacgctgcgc atgcacccgc cgggcccgct cctgtcgtgg 1200gcgcgcctcg ccgtgcacga cgcgcgcgtc ggtggccacg ccgtccccgc cgggacgacg 1260gcgatggtga acatgtgggc gatcgcccac gacgccgccg tctggccgga gccggatgcg 1320ttccgcccgg agcgcttctc ggagggggag gacgtcggcg tgctcggcgg cgacctccgc 1380ctcgcgccgt tcggcgccgg ccgccgcgtc tgccctggca ggatgctggc gctcgccacc 1440gcccacctct ggctcgccca gctgctgcac gccttcgact ggtcccccac cgccgccggc 1500gtcgacctgt ccgagcgcct cggcatgtcg ctggagatgg cggcgccgct cgtgtgcaag 1560gccgtggcta gggcctga 1578261611DNAGlycine max 26atgtcaccag attttacact cttgttcttc ccggaactca tccaaccccc tatcgtcacc 60ctccaagccg ccctatgcat ccttctctta accttcctcc tcacgttttt cctcactcca 120ggcgggcttg cgtgggcctg ggccaccaag tcctcgaccc ggcccatcat tccgggccca 180gtaatggccc tgctcagcgt cttcaccggc tccaccccgc accgcaggtt atccatgctc 240gcccgctctt accacgcaga aaagctcatg gctttttcaa tcggtctgac ccggttcgtc 300atctcgagtg aaccggaaac cgcaaaggag attctcggca gccccggttt tgctgacaga 360ccagtgaagg aatcggccta ccagcttctc ttccaccgcg caatggggtt tgcaccatac 420ggagaatact ggagaaacct gaggagaatc tccgcccttc atctcttctc tcccaagaga 480atcaccggct cggaagcctt taggaacgag gtggggttga aaatggtaga tgaagttaag 540aaggttatga aggataaccg acacgtggag gttaagagga ttttgcacta cgggtcgttg 600aacaatgtga tgatgacggt gttcggtaag tgttatgagt tctacgaggg tgagggtgtt 660gagcttgagg ctttggtgag cgaagggtat gagctgttgg gtgtttttaa ctggagcgac 720cattttccgg ttctggggtg gttggatttg cagggtgtga ggaagaggtg taggtgtttg 780gttgaaaagg ttaatgcgtt tgttgggggt gttattgagg agcatagagt gaagagggtg 840agaggtgggt gtgtgaagga tgaagggact ggggattttg ttgatgtttt gcttgatttg 900gagaacgaga acaagcttag tgaggctgac atgatcgctg ttctttggga aatgatattt 960aggggaactg acacggtggc aattctgctg gagtggatct tggctcggat ggttctccac 1020cccgacatcc aagccaaagc acagcgcgaa atagactccg tctgcggacc ctacaggctc 1080gtatccgaag cagacatgcc gaacctgcgc taccttcagg gcatagtaaa agaaactctc 1140cgcgtgcacc ctccaggccc gctactctcg tgggctcgcc tggcggtgca cgacgttacc 1200gtgggcggca agcacgtgat tcccaagggc accaccgcga tggtgaacat gtgggccata 1260acgcacgacg agaggttttg ggccgagccc gagaggttca ggcccgagcg gtttgtggag 1320gaggaggatg tgaacataat ggggtctgat ttgaggttgg cacctttcgg gtctgggaga 1380agagtgtgcc ctgggaaggc cttgggcttg gcctcggttc atctttggct cgctcagttg 1440ctacaaaatt ttcattgggt tcaatttgat ggtgtctctg ttgagttgga tgagtgtctt 1500aagctttcta tggagatgaa gaagccactt gcttgcaagg ctgtgcctag ggttgctgtt 1560tagtttatgg gtgttgttgg gttacttggg tgggtttggg ttatttgcta a 1611271603DNASorghum bicolor 27ccttctcacc tccctccccc acgccgtcgc catggacgcc accacccagg actccctcct 60cttcctcttc ccggccgccg ccaccttcct ctccccgctc ctcgccgtgc tcctcgtagc 120gctctcgctg ctctggctcg tcccaggcgg gcccgcgtgg gcgctcatct ccacctccag 180gtcccgcgcg accccgccgc cgggcgcgcc gggcgtggtc accgcgctct cgggccccgc 240agcgcaccgc gccctggcgt ccctgtcgcg gtcgcttccc ggcggcgccg cgctgtcggc 300gttctccgtc ggactcacgc gcctcgtcgt tgccagccag ccggacacgg cgcgggagct 360cctggccagc gccgccttcg ccgaccgccc cgtcaaggac gcggcccggg ggctcctctt 420ccaccgcgcc atgggcttcg caccctccgg cgactactgg cgcgcgctgt gccgcatcag 480ctccgcctac ctcttcagcc cgcgcagcga gtccgccacg gcaccccgac gcgtcaccat 540cggcgagcgc atgctgcgtg acctctccga cgccatcggc cgcctgaggc ggagcctggt 600gagcagggtg aacgtgttcg tggcgaggat catcgaagag cacaggcaga agaagaagga 660cgacgtcgcc aacaatggcg agtcggccgc cggagacttc gtcgacgtct tgctcggact 720ggagggcgag gagaagctgt cggactccga catgatcgct gtcctctggg agatgatctt 780tcgagggacc gacacggtgg cgatcctgct ggagtgggtg atggcgcgga tggtgctgca 840cccggggatc cagtccaagg cgcaggcgga gctggacgcc gtcgtgggcc gcggcggcgc 900cgtctccgac gccgacgtgt cccggctgcc ctacctgcag cgcgtcgtga aggagacgct 960ccgcgtgcac ccgccgggcc cgctgctgtc gtgggcgcgc ctcgccgtgc acgacgcggt 1020ggtcggcggc cacctcgtcc cggcgggcac cacggccatg gtcaacatgt gggccatcgc 1080gcgcgacccc gcggtgtggg cggaccccac cgcgttccgg cccgagcggt tcgaggagga 1140ggacgtgagc gtgctgggcg gggacctccg gctcgcgccg ttcggcgccg ggcggcgcgt 1200gtgccccggc aagacgctgg cgctcgccac cgtccacctc tggctcgcgc agctgctgca 1260ccgcttccag tgggcgccgg cagacggcgg cgtcgacctg gcggagcgcc tcggcatgtc 1320gctggagatg gagaagccgc tcgtgtgcaa gcccacgccg aggtggtgat ccctgaaagc 1380acaaccgagt tccaatgcat gatcatgtta ctattactag cgtttcatta cgccgcataa 1440tttgtttctt ctgagtcgag tggatcggtg ttcaatctgc ataagtggtt ttgtctatgt 1500tattgtttct gtttgtgatt gatgggatta ggtgaagagt gttcacagtg ctccatttgt 1560taggagtacc agaaatatgt gaaaaacgcc tgatgagaaa cta 1603281647DNAOryza sativa 28atggatgcca ctctcggcgc ctccactacc catggctacc tcctcctcct cccggccaac 60tccaccacct tcttctcccc gctcctcgct gccctcctcg ccgtcacctc gctgctctgg 120ctcgtcccgg gcggccccgc gtgggcgctc tcccgctgcc gccggccccc gcccggcgcg 180ccgggcgcgc tcgccgcgct cgccggcccc gccgcgcacc gcgcgctcgc ggccatgtcg 240aggtccgtgc ctggcggcgc cgccctggcg tccttctccg tcggcctcac gcgtttcgtc 300gtggccagcc gccccgacac ggcgcgggag ctcctgtcca gcgccgcgtt cgccgaccgc 360cccgtgaagg acgcggcgcg gggtttgctc ttccaccgcg ccatggggtt cgcgccctcc 420ggcgactact ggcgcgcgct ccgccgcgtc agcgccaacc acctcttcac ccctcgccgc 480gtcgccgcct cggccccgcg acgcctcgcc atcggcgagc gcatgctaga ccgcctgtcc 540gccctcgccg gcggcgagat cggcatgagg cgcgtgctcc acgcggcgtc cctggaccac 600gtcatggaca ccgtcttcgg gacgcgctac gacggcgaca gccaggaggg cgccgagctc 660gaggccatgg tgaaggaagg gtacgacctc ctcgggatgt tcaactgggg agaccacctg 720ccgctgctca aatggctcga cctgcaaggc gtgaggagga ggtgcaggac gctggtgcaa 780cgagtcgacg tgttcgtccg aagcatcatc gacgagcaca ggcagaggaa gcgccgcacc 840ggcggcaatg gcggaggcga ggagctcccc ggcgacttcg tcgacgtgtt gctcgggttg 900cagggggagg agaagatgac ggagtccgac atggtcgccg ttctctgggt aaccaaggat 960ccatctgaca tgcatgcatc tattcgatcg atcttgtgca ttgcgatcaa cggattcatg 1020gatatatttg atcttgcgcg cgtgcaggag atgatctttc gggggacgga cacggtggcg 1080attctgctgg aatggatcat ggcgaggatg gtgctgcacc cggacatcca ggcgaaggcg 1140caggcggagc tcgacgccgt cgtcggccgc gggcgcgccg tgtcggacgg cgacgtcgcc 1200ggcctgcgct acctccagtg cgtcgtgaag gaggcgctcc gcgtgcaccc gccgggcccg 1260ctcctgtcgt gggcgcgcct cgccgtgcgc gacgcgcacg tcggcggcca cgtggtcccc 1320gcgggcacca cggccatggt caacatgtgg gccatcgcgc acgacccgga gctctggccg 1380gagcccgacg agttccggcc ggagcggttc gcggaggagg acgtcagcgt gctcggcggc 1440gacctccgcc tcgcgccgtt cggcgcgggg cggcgcgcct gcccgggcaa gacgctcgcg 1500ctcgccaccg tccacctctg gctcgcgcag ctgctgcacc gcttcgagtg ggcaccggtc 1560ggcggcggcg tccacttgtt ggagcgcctg aacatgtcgc tggagatgga gaagcctctc 1620gtgtgcaagg ctaaacctag gtggtga 1647291905DNAGlycine max 29cattagtatc atcttccctc gtatacttcc cctcctcaac tttgttttgt ttttcatgta 60cgctttttga atcatcttac gtgctcctaa tcatgattcc aacacttgtt tgtattggca 120caacaatatt ccaaagcacc ctctcttctt actcattgtc tttcatctct ctttttctct 180ccacgtcact cgcccttctt gctatttccc tcaactattg gcttgtcccc ggaggttttg 240catggaggaa atatcacagt cgttacaaag gccatgcaaa agtctctggc ccaatgggct 300ggcccatatt gggaacttta cctgcgatgg gccctctagc ccacaggaaa cttgctgcca 360tggccacttc accaaaagca aaaaagctca tgacattgag tctaggaaca aatccagttg 420ttatcagcag tcacccagaa accgcaagag aaattctttg tgggtcgaac ttcgctgacc 480gacccgttaa agaatcggcc cgaatgctca tgtttgagcg tgccattgga tttgctccat 540atgggactta ttggcgccac ctacgtaaag tggcaatcac ccacatgttc tctccaagga 600ggatttctga cttggagagt ctccgacaac atgtggttgg tgaaatggtg atgaggatat 660ggaaggagat gggggacaaa ggggtggtag aggttcgagg catattgtat gaagggtctt 720tgagccacat gttggagtgt gtgtttggta ttaataattc tctaggatca caaacaaagg 780aggcgttggg tgatatggtt gaggaagggt atgacttgat tgccaagttt aattgggcag 840actattttcc tttcgggttt ttggactttc acggggtcaa gagaaggtgt cacaaattgg 900caactaaggt caatagtgtg gtgggtaaaa ttgtggaaga aagaaaaaat tcagggaagt 960acgttggaca aaatgatttt cttagtgcct tgttattgtt gcctaaagag gaaagcatag 1020gtgattcaga tgtagtggct atcttatggg aaatgatatt tcggggaaca gacacaattg 1080ctatactttt agaatggatc atggccatga tggttttaca ccaagacgta caaatgaaag 1140ctcgtcaaga gatcgactca tgcatcaagc aaaacggtta catgcgagac tcagacattc 1200caaacctccc ttacctccag gccatagtga aggaggttct ccgattgcac ccaccaggcc 1260cattactttc ctgggctcgc ctcgcaatcc atgatgtcca cgtggacaag gtcatcgtgc 1320cagctggcac aactgcaatg gttaacatgt gggctatatc acatgactca tccatttggg 1380aggacccgtg ggcctttaag cccgaaagat tcatgaaaga agatgtgtcg atcatggggt 1440cggacatgag acttgcacca tttggtgcag gacgtagggt gtgcccagga aaaacattag 1500gcttagccac agttcatcta tggcttgcac aacttcttca ccatttcata tggattccag 1560tgcaacccgt ggatctttca gaatgcctaa agctctcgct cgaaatgaaa aagcctttac 1620gatgccaagt gattcgcagg ttcaacacca taagctcttg aactcaacaa gataaattaa 1680tgcacaataa aggatatcat tatcgatgta actgttgtga taaaaaaaaa ttaaagtctt 1740tgatttgggt ggaagttatg taatgttgta aaaatatatc aagtatgtag tatgcgttga 1800gctcaagata gtccaagaaa tgggctaatg aatggattga tactatctct ctttgaaagt 1860acaccacgta caatattgga tctaataaag tcgcatggtt tttgt 1905301894DNAZea mays 30ggagagatca gctgagagct ggtgggtgct tcccatgacc ttgataccgg ccatctccgg 60cgagcaacac ggtaacatgg caaccgtagc cactagcttc gcctacctcg ccatcttcgc 120atgcctcgca tgggtgggcg cgtccctgct ctactgggct cacccaggcg gccctgcgtg 180gggcaagtac tggagggcga gggggaagaa gccgtcggcg gcgatcccgg ggcctaaggg 240gctcccggtg gtcggcagcc tcggcctcat gtccgggctg gcgcaccgct cgctggccga 300cgaggcgtcg cgccgcccgg gggccaagcg gctcatggcg ctgtcgctcg gccccgtccg 360cgcggtcgtc acgtcccacc cggacgtggc caaggagatc cttgatagcc cggcgttcgc 420cgcccgcccc ctgaaccacg ccgcgtacgg cctcatgttc caccgctcca tcggcttcgc 480cgagcacggc ccgtactggc gcgcgctccg gcgcgtcgcc gcgggccacc tgttcggccc 540gaggcaggtc gaagccttcg cgccgtaccg cgcggccgtc gccgagggca tcgtcgcggc 600cctgctgcgc gccggctccg gcggcgccgt cgtccaggtg cgcggcctcc tgcggcgggc 660gtcgctctac tacatcatgc ggttcgtgtt cggcaaggag tacgacgtgt cacgcgtggt 720gccgccgtcc ggcggggagg aggtggagga gctgctcgag atggtgcacg aggggtacga 780gctcctgggc atggagaacc tgtgcgacta cttcccgggg ctcgccgccc tcgacccgca 840gggcgtgggc gcacgatgcg ccgagctcat gccgcgggtg aaccggttcg tgcacggcgt 900catccaggag caccgtgcca aggcggtcgc cggtggagac gcgcgtgact tcgtcgacat 960cttgctctcc ctgcaggaga gtgaggggct cgccgacgcc gacatagcct ctgtgctctg 1020ggagatgatc ttcagaggaa cggacgccat ggcggtgctc atggagtgga ccctagctcg 1080cctcgtcctc caccgcgacg tccaagccaa ggcgcaccgt gagctcgaca aggtcgtcgg 1140cgcggacagc cagaccaccg agtccgcggc gccgtacctg caggcgttgc taaaggaggc 1200tctccggatg cacccgccgg ggcccctcct gtcgtggcgc cacagggcca tatccgacac 1260gtacgtcgac ggccacctcg tcccggcggg caccacggcc atggtgaacc agtgggccat 1320cagccgcgac cccgaggtgt gggacgcgcc gctcgagttc cggcccgagc ggttcctccc 1380cggcggcgag ggccaggacg tgtccgtgct cggcgccgac ggccgcctcg tgccgttcgg 1440gtccggcagg aggagctgcc ccggaaagtc cctggccatg accaccgtga cctcctggat 1500ggccacgctg ctgcacgagt tcgagtggct gccagcgtca gacgacacag gcgacgtcga 1560cctctcggag gtgctccgtc tgtcctgcga gatggcagtg ccgctggagg tccgcgtgcg 1620cccgaggagc agcgtgtgaa tgaagtgctg cctgccgata gccatgacac acccccattg 1680tgcaatgtgt agcagtgagc ctaagcgttc tgttagtgaa ctgtgaataa gcagctggtg 1740aggactgtgc acaccagctc agctcagcct ttggttcagg gtttcaactt gcccgtgtat 1800atcgtatatt tagtgtgacc gtgagtatta agttatccgc aaaaggtgta caaatcacaa 1860aagctatcga atgaagattg tgtgaagtgg tgtc 1894311914DNAGlycine max 31tttcgataaa caccattttt tctcttcctc ttttgaccaa acatgaaacc cacggcaaca 60ttcttctttc tcctatccac aacaacactc cttgtttgtc tttgcctcgg aacaacaacg 120ttccaaacca ctctctttat aaccttcttc actatttccc ttaactactg gcttgtccct 180ggaggctttg catggagaaa ctatcactct tatcacacca acgaaaaacc aaacaaaaaa 240cttactgggc ctatgggctg gcccatactt gggtctctgc ctctcatggg ctctttagcc 300caccaaaaac tcgctgcctt agccgcgacg ttaaacgcga agaggttaat ggcactgagt 360ctgggaccca ctcccgttgt tataagcagc caccccgaga ccgccagaga aatcttactc 420ggttcctcgt tttcggatcg tccgataaaa gaatccgcac gcgctctcat gttcgagcgt 480gccattggct tcgctccatc cggaacctac tggcgacacc tacgtaggat cgcggcgttc 540cacatgttct ctccgaggag gattcagggc ctggagggtc tccgacagcg cgtgggtgac 600gacatggtga agagcgcgtg gaaggagatg gagatgaaag gcgtggtgga ggttcgcggt 660gtgtttcagg aagggtctct ttgtaatatt ctggaaagtg tttttgggag taatgataag 720agtgaggagt tgggggatat ggttagggaa gggtacgagc tgattgcgat gttgaacttg 780gaggattatt ttcctttgaa gtttttggac tttcatgggg tgaagagaag gtgccacaag 840ttggcggcta aggttggtag cgtggtgggg caaattgtgg aggatcgaaa aagagaaggg 900agttttgttg tcaagaatga ttttcttagc actttgctat ccttgcccaa agaagaaagg 960ttggctgatt cagatatggc ggctattttg tgggaaatgg tgtttcgagg aacagacaca 1020gttgcaatac tccttgaatg ggtcatggcc aggatggttt tacaccaaga cgtacaaaag 1080aaagcccgcg aagaaatcga cacgtgcatc ggccaaaaca gtcacgtgcg agactcggat 1140attgcaaatc tcccatacct ccaggctata gtaaaagaag ttctccggct gcacccaccc 1200ggcccactcc tatcatgggc ccgtcttgca gtcaatgatg tccacgttga caaagttctt 1260gtgccagctg gtacaacagc aatggttaac atgtgggcca tatcacatga ctcatccatc 1320tgggaagacc catgggcttt caagcccgag aggttcctca aagaagatgt ttctatcatg 1380ggatcggact tgaggcttgc acccttcggg gctggacgta gggtgtgtcc gggccgggcc 1440ttgggtttgg ccacgaccca tctctggctc gcgcaacttc ttcgccactt catatggctt 1500cccgcgcaac ccgtggatct ttcggagtgt cttaggcttt ccatggaaat gaagacacct 1560ctgcgatgtc tagttgttcg tagataaaaa atataataac gtaagccttt tagctgaact 1620atgtttgatg tgcaattata gaatgatcga gcttgtatct gatgtttgat gttaaagatg 1680gtactcaaat atttagtgaa atatttagag atgagttatc gtataattat ggaacttatc 1740tctaagcttt atttcacttt atttttaagg gtatcaaatt attaaaataa atatctagtg 1800ccaggactag aggtggatgt aagaaaagat tggatcgatt aagaaggttt tatgtctctt 1860taaatcgtaa aatgtaaatt gctgtaatgt aatgaagttg cttccgtacg tatg 1914321310DNASorghum bicolor 32atggacttca ccgacgtcct gctctccctc aacggcgacg acaagctctc cgacgccgac 60atgatcgccg tcctctggga gatgatcttc cgaggcacgg acacggtggc ggtgctgatc 120gagtgggtgc tggcacgtct ggtgctgcac caggacgtgc agcgcaaggt gcacgacgag 180ctggaccggg tggtcgggcc gggcgaggcc gtgacggagt cggacaccgc ctcactggtg 240tacctgcagg cggtcatcaa ggaagtgctg cgcctgcacc cgccgggccc actgctgtcc 300tgggcgcggc tggccacgtc ggacgtgaac gtcggcgggc acctggtccc cgcgggcacc 360accgccatgg tgaacatgtg ggccataacc cacgacgcca gcgtgtggcc tgagccgacg 420gagttcaggc ccgagaggtt cgtcgccgcc gccggcggcg aggacgtcgt cccgataatg 480ggttcggacc tccggctcgc gccgttcggg tccggcaggc ggagctgccc cgggaagtcg 540ctcgcggtgg ccaccgtcgg gttctgggtc gccaccctgc tgcacgagtt cgaatggttg 600ccgtgcggcg gcggcggcgg cgtggacctg tccgaagtgc tgaggctgtc gtgtgagatg 660gccgcgccgc tggaggcgag ggtggtgcca cgtcgtcacg cggtgtgatg atgacgaggc 720agctgagaca cacgtggacg tggctgggga gaggggacgg agtggctagc ttcttctact 780actactacct taccaccttc tagcagaacg taacgtacgt ggccccggac gacgatcgat 840gagcgagatg cgtaaaaaaa aaatcgccca agtgccatgc tttagagctg atgctggtgt 900ggacttccag taatttcctg tgtatgatgg taggtgctgg tggaaggact acgtagtaac 960caccagccgg tttgatgctt aattatgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 1020gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 1080gtgtgtaatg taacttacta gctgctgctg ctgcatgtaa tatctgcctg ctgttagtcg 1140actactaggt agtgtagtgt accagatgat gagtgtgaca gttatctttt ccttttcagt 1200aatgcgtagc tagctagagc tagatgtgta ttattgtgta ataataatcc tatgtgtgta 1260tatatctctc tatagtttct atcctaatgc aagcgtgcgt gtgtgtacgt 1310331852DNAGlycine max 33atgaaaccca cggcaacatt cttctttctc cttccttcaa caacactcgt tgtttgtctt 60tgccttggaa ttggaacaac caccctcttt ataaccctcc tcgcaatttc ccttaactac 120tggcttgtcc ctggaggctt tgcatggaga aactatgact attatcaaac caaaaaaaaa 180cttactgggc ctatgggctg gcccatactc gggactctgc ctctcatggg ctctttagct 240caccaaaaac ttgcggcttt agccacttcg ctgaacgcaa agaggttaat ggcgctgagt 300ctgggcccca ctcccgttgt tataagcagc caccccgaga ccgctagaga aatcttgttg 360ggttcatcgt tttcggatcg tccgataaaa gaatcggcac gcgctctcat gttcgagcgt 420gccattggtt tcgctcattc aggaacctac tggcggcacc tacgtaggat cgcggcgttt 480catatgttct ctccgaggag gattcatggc ttggagggtc tccgacaacg cgtaggtgac 540gacatggtga agagcgcgtg gagggagatg ggggagaagg gggtggtgga ggttcggagg 600gtatttcagg aagggtcact ttgtaatatt ttggagagtg tttttggaag taacgataag 660agtgaggagt tgagggatat ggttagggaa gggtacgagt tgattgcgat gtttaacttg 720gaggattatt ttcccttcaa gtttttggat tttcatgggg tgaagagaag gtgccacaag 780ttggcggcta aggttggtag tgtggtgggc caaattgtgg aggaacgaaa aagagatggt 840ggttttgttg ggaagaatga ttttcttagc actttgctat ccttgcccaa agaagaaaga 900ttggctgatt cagatctggt ggctattctg tgggaaatgg tatttcgagg aacagacaca 960gttgcaatac tccttgaatg ggttatggca aggatggttt tacaccaaga cttacaaaag 1020aaagcccgcg aagaaatcga cacgtgcgtc ggccaaaaca gtcacgtgcg agactcggat 1080attgcgaatc tcccttacct ccaggccata gtgaaagaag ttctccggct gcacccacca 1140ggcccgctac tatcatgggc tcgccttgcg gtccatgatg tccatgcgga caaagtcctc 1200gtgccagctg gcacaacagc aatggttaac atgtgggcca tttcgcatga ctcgtccatc 1260tgggaagacc catgggcttt caagcccgag aggttcctca aagaagatgt ttccatcatg 1320ggatcggact tgaggcttgc acccttcggg gctggacgta gggtgtgtcc gggccgggcc 1380ttgggcttgg ccacggccca tctctggctc gcgcaacttc ttcgccactt catatggctt 1440cccgcacaaa ccgtggatct ttccgaatgc cttaggcttt ccatggaaat gaagacacct 1500ctgcgatgcc tagtggttcg tagataaata aataaaaaat cgtaagccct tgaaccgaac 1560catgtttaat gtgcaattag gatgatcgag ctttgtatct gttgtttgat gttaaatttg 1620gtactcaaat atctagtgaa atatttagag atgagtttat cgtataatta gggagcttat 1680ctctaagttt tatttcactt tatttttaaa gggtatcaaa ttattaaaat aaatatctag 1740tgccagttga ctagaggtgg gtgtaagaag agagtggctt aagaaggctt tatgtatctt 1800taaattgtaa aatgtaaatt actgtaatgt aatgcatgca tttttcttct tg 1852341554DNASorghum bicolor 34atggtgctca ccatggccac cggccaagag gactccctcc tcctgctcct cctccccacc 60acctccccac tcccgcccct catggccgtg ttcatcctag ccgccgtcct cctgtggctc 120tcccccggtg gtcctgcgtg ggcgctctcc cgctgccgcc gcccgccgtc cggcccaacg 180ggcgtggtca ccgcgctctc aagccccgtg gcgcaccgca ccctggcggc gctgtcccac 240gccgtagacg gcggcaaagc actgatggcc ttctcggtcg ggctcacccg cctcgtcgtg 300tcgagccagc ccgacacggc gcgagagatc ctcgtcaacc ccgcgttcag cgaccgcccc 360atcaaggacg cggctcgcca cctgctcttc caccgcgcca tgggcttcgc gccctccgga 420gacgcgcact ggcgcgggct ccgccgcctc gccgccaacc acctgttcgg cccgcgccgc 480gtggcggcgg

ccgcgcacca ccgcgtctcc atcggcgagg ccatggtcgc cgacgtcgcc 540gctgccatgg cccgccacgg cgaggtctcc ctgaagcgcg tgctgcatat cgcgtctctc 600aaccacatca tggccaccgt cttcggcaag cactacgaca tggacagcca agagggcgtc 660cttctggaag agatggtcac cgagggctac gacctcctgg gcacgttcaa ctgggctgac 720cacctgccgt tgatcaagca tctcgacctc cagggcgtgc gccgccggtg caacaggtta 780gtccaaaagg ttgaagtgtt cgttggaaag atcatccagg agcacagggc gaggcgcgcg 840aatggaggag tcgacgatga gtacatgggt gacttcgtcg acgtccttct tgacctcgag 900ggagaggaga agctgtccga atccgacatg atcgctgttc tttgggagat gatcttcaga 960ggcgccgaca ctgtggcgat cttgatggag tggatcatgg cgaggatggc gctgcaccct 1020gaaatccagt ccaaggccca ggcggagctg gacggcgtcg tcgtgggcgg cgtggcggac 1080gccgacgtgg gcaacctccc ctacatccag tgcatcgtga aggagacgct gcggatgcac 1140cctccgggcc cgctcctgtc gtgggcgcgc ctcgccatcc acgacgcgca cgtcggcggc 1200cacctggtcc ccgccggcac cacggccatg gtgaacatgt ggtccatcgc gcatgacccc 1260gccatctggg cagagccgga gaagttccgc cccgagcgct tccaggagga ggacgtgagc 1320gtcctcggga gcgacctccg cctggccccg ttcggcgccg gacgccgcgt ctgccccggc 1380aagatgctgg ccctcgccac cacacacctc tggatcgccc agctgctgca cgagttcgag 1440tgggcacccg cggccgccaa cggcggcgtc gacctgtccg agcgcctcaa catgtcgctg 1500gagatggcca cgccgctggt gtgcaaggcc gtccccaggg cccagctggc ctaa 1554351882DNASorghum bicolor 35cgcaacgcaa cagctagcag caagctctga aagtcgcaat ggagagctca gttgagagct 60ggtgggtgct tcccatgacc ttgatcccgg ccatctccgg ccagcagcac gagaacatgg 120ccaccatagc cactagcttc gtctacctcg ccatcttcgc atgccttgca tgggcaggcg 180cgtccctgct ctactgggct cacccaggcg gccctgcgtg gggcaagtac tggagggcga 240aggggaagcc gtcgtcgacg atcccggggc ccaaggggct cccggtcgtc ggcagcctcg 300gcctcatgtc cgggctggcg cactgctcgc tggccgacga ggcgtcgcgc cggccggggg 360ccaagcggct catggcgctg tcgctcggcc ccgtccgcgc ggtggtcacg tcccacccgg 420acgtggccaa ggagatcctc gacaacccgg cgttcgccga ccgccccctc aaccacgccg 480cgtacggcct catgttccac cgctccatcg gcttcgccga gcacggcccg tactggcgcg 540cgctccggcg cgtcgccgcg ggacacctgt tcggcccgag gcaggtcgag gccttcgcgc 600cgtaccgtgc cgccgtcggg gaagggatcg tcgcggccct gcacggcgcc ggcggcggcg 660tcgtccaggt gcgcggcctc ctgcgccgag cttcgctcta ctacatcatg cggttcgtgt 720tcggcaagga gtacgacgtg tcgcgcgccg tgccggcgtc cgggaaggag gaggtggagg 780agctgctcga gatggtgcac gaggggtacg agctcctggg catggagaac tggtgcgact 840acttcccggg gctcgccgcc ctcgacccgc agggcgtcgg agcacggtgc gccgagctca 900tgccacgggt gaaccggttc gtgcatggca tcatccagga gcgccgtgcc aaggcgatcg 960ccggaggaga cgcgcgtgac ttcgttgaca tcttgctctc cctgcaggag agcgagaggc 1020tcgccgacgc cgacatagcc gctgtgctct gggagatgat cttcagagga acggacgcca 1080tggccgtgct catggagtgg accctagctc gcctcgtcct ccaccgtgac gtccaagcca 1140aggcgcaccg tgagctcgac gaggtcgtcg gcgggaacag ccaggtcgtc accgagtcgg 1200cagcggcgcc gtcgctgcct tacctgcagg cgttgctaaa ggaggctctc cggatgcacc 1260cgccggggcc cctcctgtcg tggcgccaca gggcgatatc cgacacgtac gtcgacgggc 1320acctcgtccc ggcaggcacc accgccatgg tgaaccagtg ggcaatcagc cgcgaccccg 1380aggtgtggga cgcgccgctc gagttccggc ccgagcggtt cctccctggc ggcgagggcc 1440aggacgtgtc cgtgctcggc gccgacggcc gcctcgtgcc gttcgggtcc ggtcggagga 1500gctgccccgg caagtccctg gctatgacca ccgtgaccac ctggatggcc accctgctga 1560acgagttcga gtggctgccg gcgtcagacg atacaggcgg cgacgtcgac ctctcggagg 1620tgctccgtct gtcctgcgag atggctgtgc cgctggaggt ccgcgtgcgc ccgaggagcg 1680gcatgtgaat gaagtatctg ccgatagcca tggcaccccc attgtgcaat gtgtagcagt 1740aaccctaagc gttgtgttag taaactgtga ataagcagca gctggtgagg actgtgcata 1800ccagctcagc tcagttcagt atttggttca gggtttcaac ttgcctatgt atcatatata 1860tagtgtgacc gtgagtacaa gt 1882362085DNASorghum bicolor 36gtgcccactt cttccaactt ataatccaag caaccaacta tagctcaaca catctctcct 60tgtgttctca tctatagcca tctaagtagc aattgcatta gcaaccatag tagcacctgc 120tatgcactag tcagtaatca gtaccacaca tagtttacac acacacaaca cacacaaacg 180cacacacaaa aaagacttta cacacttagg ttaactatac atatagccat gaagactgaa 240gtaatcacca ccatgatctc cttggtcttc ctcgtgcatt tcgctatcac catcagccct 300aacgcacaac cttcatggct cttctctctc atgtctctct cccttgccgt ggtggcggtc 360atagtgccat tagtagtcac caccacttgc catgcacgta agaataccga cgctaccacc 420accatcccgg ggccacgagg gtggccgctg gtaggctccc tgctggtcgt gtcgggccca 480ctcatgcacc gtcgcctagc cgcgctggcc gacgcgcata gcgcacgtcg cctcatgtcg 540ctcaccctcg gtgccacccc cgtggtgatc agcagccacc cagaaacggc gcgagacatc 600ctctcaggtg ccgccttcgt ggaccgcccg cctaaggccg cagcccggga actcatgttt 660tgccgtgcca tcgggtttgc ccccactggg gagtattggc gtcgcctccg tcgcatcacg 720ggcgctggca tgctctcccc gcgtcgcatg gccatgctca ggggtcttcg ctgccgtgtt 780gccgacagca tgatccagcg tgtcgcggac cagatggaga ggtccgggga ggtggccatg 840agagccttgc tccaaagggc ctccctagag agcatggtgg gtagcgtgct aggcctcgag 900ggtgacgctg tttgtgagga gctgggtgag atggtgaggg aagggtatga gctcgtgggc 960atgttcaacc tagaagacca ctactacaag acatcatggg gcccgttgat ggacctttgg 1020ggggtgaggc ccatgtgcag ggagctggct gctatggtta gagggtattt tgggaagatc 1080attcaggaga ggaggctggc aggggactgc cacgagaggg ccgacttgct tagctatatg 1140ctttcacttc cagaggagga gaagttggag gactctgatg tgattgctgt gctgtgggag 1200atgattttcc gtggcgtgga cgtcgtggcg attctcctgg agtgggccat ggcccgcatg 1260tcactgcacc cagacatcca atccaaggcc caggaggaga tggacgcagc ggtgggcgtc 1320cgtcgtcgtc gtgccatcac cgactccgac gtccccaacc ttgccttcct ccaatggatc 1380ctcaaggaga cgctccggat gcacccgccg ggcccgctgc tctcctgggc gcgcctggcg 1440gtgcaggacg cgcgggtggg caagcacgtg gtgccggcgg ggacgacggc catggtgaac 1500atgtgggcca tctcgcacga cgaggccatc tggggagacc cctgggtgtt ccggccggag 1560aggttcgcgg cggcggcggc cggggaggag gtgagcgtgc tcgggtccga cctcaggctg 1620gcgccgttcg ggtccggccg gagggtgtgc cctggcagga tgatgggcct cgccaccgcg 1680cagctctggc tcggacgcct cctgcaggag taccggtggc tgccgccgcc ggccaataag 1740cccgtcgagc tcgccgagtg cctccgcctg tccatggaga tgaagacgcc cctcgtctgc 1800cgcgcggttc ctcgtcgtcg cggaggacgg cctcctgctg cagcttgatg ggccactgtg 1860atgatgagct ccttcggtgc tgtggccact gttgggcacc agtgtaaaat taaatccttg 1920tgtgtttgcc tactactcgt ttacagtgtt gtttgtacgt acaatcaaag attattgtat 1980gtatgtgatg tgatgtgatg tatctgtgcc cggccggcct atagctacgt ggctagacac 2040atgagtatag tttgcactac tctgatatat atatatgtgt tatac 2085371500DNAOryza sativa 37atggcggtcg tcgccttgcc gccgcttctt gcgaaacgcc atggacatgc acgccgggtt 60aatggcggcg gcgctgccat tcccgggccg cgggggtggc cgctgctggg gtcgctcccg 120gtggtgtccg gtccgctcat gcaccgccgc ctcgccgcgc tggccgacgc gcacggcggc 180ggcgcgcggc gcctgatgtc gctgacgctc ggcgcgacgc ccgtggtggt gagcagccac 240ccggacacgg tgcgggagat cctcgccggc gccgcgttcc gcgaccgtcc cgccagggcc 300gcggcgcggg agctcatgtt cctccgcgcc gtcggcttcg ccccggcctc cggagacgac 360ggcggcgcct actggcgccg cctccgccgc gccgcgggcg cgggcatgct ctccccgcgc 420cgcgccgccg cgctcgccgc gctgcgcgcc cgcgtcgcgc gccgcacgtc cgaggccgtg 480tcccgcggca tggccgtgcc gcccggccgc gtcgccatgc gcgccctcct ccacgccgcc 540tccctcgaca acatggttgg cagcgtgcta gggctcgaac accatgacca ccatggcggc 600gtcatcagcg acatgggtga catggtgagg gaagggtacg agctggttgg caagttcaac 660ctaggagact actacagtac tacacagtac cagtgcctgt gggggttgct ggatttccat 720ggggtggggc ccaggtgtca gaggctggca gctagggtta gggagcagtt tgggagggtg 780atggaggaga ggaggaaggt gagtgacctg cacaagaggg atgatcttct tagctacatg 840ctctccatgc cacaggagga gaggattgag gactctgatg tcattgctgt cctctgggag 900atgatctttc gtgggacaga tgtagttgca atactcctgg aatgggccat ggcccggatg 960gtactccacc cagacatcca gtccaaggtg caagaagaac tagatagggc ggtgggccac 1020cggcccatga ccgactcgga catccccaac cttcgcttcc tccattgtgt catcaaggaa 1080accctccgca tgcacccgcc tggcccactt ctctcatggg cccgcttggc ggtgcatgat 1140acctatgtgg gcaagcacct agtacccgca gggactacgg caatggtgaa tatgtgggcc 1200atatcccatg atgagacgat atggggtgac ccatgggtgt ttcgacccga aaggtttatg 1260gaagaggata tcaatgtgtt gggatcagat ttaaggttgg caccgtttgg atcaggtcgt 1320cgggtgtgcc ctggacggat gatgggtctc tccactgcct atctatggtt tggccggatg 1380ttgcaagagt ataagtgggc agcggctcag ccggttaaac ttacggagtg cctccgtctt 1440tctatggaga tgaagaaacc tttggtttgt catgcagttc ctcgtagcaa aactggctaa 1500381668DNAOryza sativa 38atggcaatgg ccaccgccac cgcctcctcc tgcgtcgacg ccacgtggtg ggcgtacgcc 60ctcccggcgc tcctcggcgc cgacaccctc tgcgcccacc cggcgctgct cgccggcgcc 120gtcctcctgg ccttcgccac cgccgcggtg ctcgcctggg ccgcgtcccc cggcgggccg 180gcgtgggcgc acggccgcgg ccgcctcggc gcgacgccca tcgaggggcc ccgggggctc 240cccgtgttcg gcagcatctt cgcgctctcc cggggcctcc cgcaccgcgc gctcgacgcg 300atgtcgcgcg acgcggcggc gccacgggcg agggagctca tggcgttctc cgtcggggag 360acgccggcgg tggtgtcgtc gtgcccggcg acggcgaggg aggtgctcgc gcacccgtcg 420ttcgccgacc gcccgctgaa gcgctcggcg cgggagctgc tgttcgcgcg cgccatcggg 480ttcgccccca gcggcgagta ctggcgcctc ctccgccgca tcgcctccac ccacctcttc 540tcccctcgcc gcgtcgccgc gcacgagccg gggcgccagg ccgacgccac ggcgatgctg 600tccgccatgg ccgccgagca gtccgccacc ggcgccgtcg tgctccgccc ccacctccag 660gccgccgcgc tcaacaacat catgggcagc gtgttcggcc ggcgctacga cgtctcctcc 720tcctccggcg ccgccgccga cgaggccgag cagctcaaga gcatggtgcg cgaggggttc 780gagctcctcg gcgcgttcaa ctggtccgac cacctcccat ggctcgccca cctctacgac 840cccaaccacg tcgcccgccg ctgcgccgcg ctcgtccccc gcgtccaggc gttcgtccgc 900ggcgtcatcc gcgaccaccg cctccgccgc gactcctcct ccaccgccgc cgacaatgcc 960gacttcgtcg acgtcctcct ctccctcgag gcccacgaga acctcgccga ggacgacatg 1020gtcgccgtcc tctgggagat gatatttcgt gggacggaca cgacggcgtt ggtgacggag 1080tggtgcatgg cggaggtggt gaggaacccg gcggtgcagg cgaggctgag ggcggaggtg 1140gacgcggcgg tgggcggcga cgggtgtccc agcgacggcg acgtggcgcg gatgccgtac 1200ctgcaggcgg tggtgaagga gacgctgagg gcgcacccgc cggggccgct gctgagctgg 1260gcgcggctgg ccaccgccga cgtggggctc gccaacggca tggtggtgcc ggcgggcacg 1320acggcgatgg tgaacatgtg ggccatcacc cacgacggcg aggtgtgggc cgacccggag 1380gcgttcgcgc cggagcggtt catcccgtcg gagggcggcg ccgacgtcga cgtccgcggc 1440ggcgacctcc gcctggcgcc gttcggcgcc gggcgccgcg tctgccccgg caagaacctc 1500ggcctcgcca ccgtcaccct ctgggtcgcc cgcctcgtcc acgccttcga ctggttcctc 1560cccgacggct cgccgccggt gtccctcgac gaggtcctca agctctccct cgagatgaag 1620acccctctcg ccgccgccgc caccccccgc cgccgccgcg ccgcctga 166839525DNASorghum bicolor 39cgtgacggac gccgacgtgg cgaacctccc ctacgtgcag agcgtcgtga aggagacgct 60gcgcatgcac ccgccagggc ccgctgctgt cgtgggcgcg cctggccatc cacgacgcgc 120acgtcggcgg ccaccctggt ccccgccggc accacggcca tggtgaacat gtgggcgatc 180gcgcacgacc ccgccatctg ggcggagccg gaggagttcc gccccgagcg gttccaggag 240gaggaggagg acgtgagcgt cctcggcggc gacctccgcc tggccccctt cggtgccggc 300cgccgcgtat gccccgacaa gatgctcgcc ctcgccacca cccacctctg ggtcgcccag 360ctgctgcacc ggttcgagtg ggcccctgcg ggcgccgcca gcagcggcgg cggcggcgtc 420gacctgtcgg agcgcctcaa catgtcgctg gagatggcca cgccgctggt gtgcaaggcc 480gtgcccaggt cagcccccca gctgcatgca ggcctagcta gctaa 525402076DNASorghum bicolor 40atggcgatgg cctccgcagt ttcgtcgtgc acggacagca cgtggtgggt gtacgcgctc 60ccgacgctgc tcggctcgga caccctgtgc gcccacccgg ccctcctggc cggcctgctc 120ttcctgacca ccgtcacggc ggctctgctg gcgtgggccg cgtcgccggg agggcctgcg 180tgggcgcacg gccgcggccg cctcggcgcc actcctatcg tgggtccccg gggtctcccc 240gtgttcggca gcatcttcgc gctctcccgc ggtctgccgc accgtaccct cgccgcgatg 300gcccgcgcgg cggggccccg ggccaaggag ctcatggcgt tctccgtcgg ggacacgccg 360gcggtcgtgt cgtcgtgccc ggccacggcg cgtgaggtgc tcgcgcaccc gtccttcgcc 420gaccgccccg tgaagcggtc ggcgcgggag ctcatgttcg cgcgcgccat cgggttcgcg 480cccaacggcg agtactggcg ccgcctccgc cgcgtcgcgt ccacgcacct cttctccccg 540cgccgcgtcg ccgcgcacga gccgggacgc cagggcgacg cggaggccat gctccgctcc 600gtcgccgccg agcagtccgc ctcgggcacc gtcgtcctcc gcccgcacct ccaggccgcc 660gctctcaaca acatcatggg cagcgtcttc ggcacgcgat acgacgtcac atccggcgcc 720accgccggcg ccgcggaggc cgagcagctc aagagcatgg tgcgcgaggg gttcgagctc 780ctcggcgcct tcaactggtc cgaccacctc ccctggctcg cccacctgta cgaccccagc 840aacgtcaccc gccgctgcgc cgcgctcgtc ccgcgcgtcc agaccttcgt ccgtggcgtc 900atcgacgagc accggcgccg ccgccaaaac tccgccgccc tcgacctcaa cgacaatgct 960gacttcgtct acgtgctcct ctccctcgac ggcgacgaga agctccgcga cgacgacatg 1020gtcgccatcc tctgggagat gatcttccgc ggtacggaca cgacggcgct tctgacggag 1080tggtgcatgg cggagctggt gcgccacccg gcggtgcagg cgaggctgcg ggccgaggtg 1140gacgcagctg tcggcgccgg cggtcgcccc accgacgccg acgtggcgcg catgccgtac 1200ctgcaggcgg tcgtgaagga gacgctgcga gcgcacccgc ctggcccgct gctgagctgg 1260gcgcgcctcg ccaccgccga cgtgcccctc tccaacggca tggtggtccc ggccggcacc 1320acggcgatgg tgaacatgtg ggccatcacc cacgacgccg gcgtgtgggc cgacccggac 1380gcgttcgcgc cggagcggtt cctgccctcc gagggcggcg ccgacgtgga cgtccgcggc 1440gtcgacctcc gcctggcacc gttcggcgcc gggcgccgcg tctgccccgg caagaacctg 1500gggctcacca ccgtgggcct ctgggtcgcc cgcctcgtgc acgccttcga gtgggcgctg 1560cctgatggcg cgccgcccgt ctgcctcgac gaggtcctca agctctccct ggagatgaag 1620acgccgctcg ccgccgcggc catcccgcgc accgcatgat ccatcctgcc gctgccgccg 1680acgcgtgcaa caagaaccga ttatgctttg tcacgtcacg tcacgcgttc tttgtctgtg 1740tgtgtgtggt tctaagctag tgtgcttctt cttgtcgatc gtcggttcgt tctcgtgcct 1800gcctttgcct agggtttcgt ttcttgcaaa gtagtgacag tgtctccctt agagtcatca 1860acggggctcc aattttggaa aggtgcgtgt taggagttaa cccctagaca tgtctgcgtc 1920tcgatcacca cctactatgt cattatcagc gcagcaccta tatatagatc agtgtctgtc 1980gatcagtcat ggaagtcgat cgtgtgctca agtctgctgt attatatata atgtattgta 2040atgtgattat caagaaccgt gctatctata tgttgc 2076411937DNABrachypodium distachyon 41atggagagct cagtcgagag cagctggtgg gtgctgcccc tgaccttgat tcctgccatc 60tccggccagc agcagcagca cgatcaaagc acggccgccg ccatagccac cagcttcgtc 120tacctcgcca tcctcgcctg cctcgcctgg gcggccaagt ccctgctcta ctgggctcac 180ccgggcgggc ctgcatgggg ccggcggtac tggacgagcc cgtgcgcgaa gacggctccg 240gctccggcgc cgatccccgg gccgagaggg ctcccggtgg tgggcagcct gggcctgatg 300tccggactag cccacagcac gctggccgcc gaggcggcaa ggacgccggg cgcgaagcgg 360ctcatggcgc tgtctctcgg cccagtcccc gccgtcgtca cggcccaccc ggacgtggcc 420aaggagatcc tcgacaaccc ggcgttcgcg gaccggcccg tgaaccacgc cgcctacggc 480ctcatgttcc accgctccat cggcttcgcg gagcacggcc cctactggcg cgcgctccgg 540cgcgtggcat cggcgcacct gttcgcgccc aggcaggtcg acgccttcgc cccttaccgc 600gcgcgcgtcg gggaagacgt cgtggccgcg ctccgccatg ccgggggcgg cgtcgtgaac 660gtgcgcggcg tgctccggcg cgcgtcgctc tactacatca tgcgcttcgt gttcgggaaa 720gagtacgacg tgtcgtcgga ctcggggaag aaggatcagg gggaagtgga ggagctgctg 780gagatggtgc atgagggtta tgagctgctg gggaaggaga actggtgcga ctacttcccg 840gggctggcgg ggttcgaccc gcagggcgtc ggggcgcggt gcgccgagct catgccgcgg 900gtgaaccgct tcgtgcacgg catcatcgat gagcaccgcg gcaaggcgat gatagccgga 960ggagaaggag aggcgcagcc gctggacttt gtggacatac tgctttcgtt gcaggagagc 1020gaggggctcg ccgacgccga catcgccgcc gtgctctggg agatgatctt cagaggaaca 1080gacgccatgg cggtgctgat ggagtggacc atggcacgcc tcgtcctgca ccccggcgtc 1140caagccaacg tgcacaagga gctggacgag gtggtcggca agagcagcca cgtcaccgag 1200tcagccgtgc tctcactgcc ttacctgcag gcgctgctca aggaggcgct ccgcgtgcac 1260ccgccggggc cgctgctgtc gtggcgccac agggccatgt gggacaccta cgtggacggc 1320cacctggtcc cggcgggcac cacggccatg gtgaaccagt gggccatgag ccgggacccg 1380gaggtttggg ccgagccgct cgagttccgg cccgaacggt tcctcccggg cggcgaggcc 1440ggcccgggag tctccgtgct cggctcggac ggccggctcg tgccgttcgg gtctggacgg 1500aggagctgcc ccgggaagaa cctggccatg accaccgtca cggcgtggat ggccacgctg 1560atgcacgagt tcgagtggat gccggccaag accggcgccc ccgtcgacat gtcggaggtg 1620ctccgcctgt catgcgagat ggcgacgccg ctccaggtcc gggtgcgccc caggcgcggc 1680gtttgaaagt ctgaggctgc tttcgacggc catatatgac ttcaccgtgt agtttctttc 1740ttactagccg tgaccctggg ttgtgcttcc tgtttgtgaa taagctggct gggatgaaca 1800aaagtgtgca ccggctcagc ttcagtgttt ggttcagagt tttctttttg aacttcgtca 1860gaagtatcat caggtgtgag cttgaggttc cacgttggtg tacagattgc aagaagaaaa 1920tctataaagg attgtgc 193742530PRTArabidopsis thaliana 42Met Ala Thr Lys Leu Glu Ser Ser Leu Ile Phe Ala Leu Leu Ser Lys1 5 10 15Cys Ser Val Leu Ser Gln Thr Asn Leu Ala Phe Ser Leu Leu Ala Val 20 25 30Thr Ile Ile Trp Leu Ala Ile Ser Leu Phe Leu Trp Thr Tyr Pro Gly 35 40 45Gly Pro Ala Trp Gly Lys Tyr Leu Phe Gly Arg Leu Ile Ser Gly Ser 50 55 60Tyr Lys Thr Gly Asn Val Ile Pro Gly Pro Lys Gly Phe Pro Leu Val65 70 75 80Gly Ser Met Ser Leu Met Ser Ser Thr Leu Ala His Arg Arg Ile Ala 85 90 95Asp Ala Ala Glu Lys Phe Gly Ala Lys Arg Leu Met Ala Phe Ser Leu 100 105 110Gly Glu Thr Arg Val Ile Val Thr Cys Asn Pro Asp Val Ala Lys Glu 115 120 125Ile Leu Asn Ser Pro Val Phe Ala Asp Arg Pro Val Lys Glu Ser Ala 130 135 140Tyr Ser Leu Met Phe Asn Arg Ala Ile Gly Phe Ala Pro His Gly Val145 150 155 160Tyr Trp Arg Thr Leu Arg Arg Ile Ala Ser Asn His Leu Phe Ser Thr 165 170 175Lys Gln Ile Arg Arg Ala Glu Thr Gln Arg Arg Val Ile Ser Ser Gln 180 185 190Met Val Glu Phe Leu Glu Lys Gln Ser Ser Asn Glu Pro Cys Phe Val 195 200 205Arg Glu Leu Leu Lys Thr Ala Ser Leu Asn Asn Met Met Cys Ser Val 210 215 220Phe Gly Gln Glu Tyr Glu Leu Glu Lys Asn His Val Glu Leu Arg Glu225 230 235 240Met Val Glu Glu Gly Tyr Asp Leu Leu Gly Thr Leu Asn Trp Thr Asp 245 250 255His Leu Pro Trp Leu Ser Glu Phe Asp Pro Gln Arg Leu Arg Ser Arg 260 265 270Cys Ser Thr Leu Val Pro Lys Val Asn Arg Phe Val Ser Arg Ile Ile 275 280 285Ser Glu His Arg Asn Gln Thr Gly Asp Leu Pro Arg Asp Phe Val Asp 290 295 300Val Leu Leu Ser Leu His Gly Ser Asp Lys Leu Ser Asp Pro Asp Ile305 310 315 320Ile Ala

Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala 325 330 335Val Leu Ile Glu Trp Ile Leu Ala Arg Met Val Leu His Pro Asp Met 340 345 350Gln Ser Thr Val Gln Asn Glu Leu Asp Gln Val Val Gly Lys Ser Arg 355 360 365Ala Leu Asp Glu Ser Asp Leu Ala Ser Leu Pro Tyr Leu Thr Ala Val 370 375 380Val Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu Leu Ser Trp385 390 395 400Ala Arg Leu Ala Ile Thr Asp Thr Ile Val Asp Gly Arg Leu Val Pro 405 410 415Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Val Ser His Asp Pro 420 425 430His Val Trp Val Asp Pro Leu Glu Phe Lys Pro Glu Arg Phe Val Ala 435 440 445Lys Glu Gly Glu Val Glu Phe Ser Val Leu Gly Ser Asp Leu Arg Leu 450 455 460Ala Pro Phe Gly Ser Gly Arg Arg Ile Cys Pro Gly Lys Asn Leu Gly465 470 475 480Phe Thr Thr Val Met Phe Trp Thr Ala Met Met Leu His Glu Phe Glu 485 490 495Trp Gly Pro Ser Asp Gly Asn Gly Val Asp Leu Ser Glu Lys Leu Arg 500 505 510Leu Ser Cys Glu Met Ala Asn Pro Leu Pro Ala Lys Leu Arg Arg Arg 515 520 525Arg Ser 53043534PRTArabidopsis thaliana 43Met Ala Thr Lys Leu Asp Thr Ser Ser Leu Leu Leu Ala Leu Leu Ser1 5 10 15Lys Cys Ser Leu Leu Thr Gln Thr Asn Leu Ala Leu Ser Leu Leu Val 20 25 30Ala Ser Leu Ala Ser Leu Ala Leu Ser Leu Phe Phe Trp Ser His Pro 35 40 45Gly Gly Pro Ala Trp Gly Lys Tyr Phe Leu His Arg Arg Arg Gln Thr 50 55 60Thr Val Ile Pro Gly Pro Arg Gly Leu Pro Phe Val Gly Ser Met Ser65 70 75 80Leu Met Ser Asn Thr Leu Ala His Arg Cys Ile Ala Ala Thr Ala Glu 85 90 95Lys Phe Arg Ala Glu Arg Leu Met Ala Phe Ser Leu Gly Glu Thr Arg 100 105 110Val Ile Val Thr Cys Asn Pro Asp Val Ala Lys Glu Ile Leu Asn Ser 115 120 125Pro Val Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Ser Leu Met 130 135 140Phe Asn Arg Ala Ile Gly Phe Ala Pro Tyr Gly Val Tyr Trp Arg Thr145 150 155 160Leu Arg Lys Ile Ala Ser Asn His Leu Phe Ser Pro Lys Gln Ile Lys 165 170 175Arg Ser Glu Thr Gln Arg Ser Val Ile Ala Asn Gln Ile Val Lys Cys 180 185 190Leu Thr Lys Gln Ser Asn Thr Lys Gly Leu Cys Phe Ala Arg Asp Leu 195 200 205Ile Lys Thr Ala Ser Leu Asn Asn Met Met Cys Ser Val Phe Gly Lys 210 215 220Glu Tyr Glu Leu Glu Glu Glu His Glu Glu Val Ser Glu Leu Arg Glu225 230 235 240Leu Val Glu Glu Gly Tyr Asp Leu Leu Gly Thr Leu Asn Trp Thr Asp 245 250 255His Leu Pro Trp Leu Ser Glu Phe Asp Pro Gln Arg Ile Arg Ser Arg 260 265 270Cys Ser Asn Leu Val Pro Lys Val Asn Arg Phe Val Asn Arg Ile Ile 275 280 285Ser Asp His Arg Glu Gln Thr Arg Asp Ser Pro Ser Asp Phe Val Asp 290 295 300Val Leu Leu Ser Leu Asp Gly Pro Asp Lys Leu Ser Asp Pro Asp Ile305 310 315 320Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala 325 330 335Val Leu Ile Glu Trp Ile Leu Ala Arg Met Val Leu His Pro Asp Ile 340 345 350Gln Ser Thr Val His Asn Glu Leu Asp Gln Ile Val Gly Arg Ser Arg 355 360 365Ala Val Glu Glu Ser Asp Val Val Ser Leu Val Tyr Leu Thr Ala Val 370 375 380Val Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu Leu Ser Trp385 390 395 400Ala Arg Leu Ala Ile Thr Asp Thr Ile Ile Asp Gly Arg Arg Val Pro 405 410 415Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ala His Asp Pro 420 425 430His Val Trp Glu Asn Pro Leu Glu Phe Lys Pro Glu Arg Phe Val Ala 435 440 445Lys Glu Gly Glu Val Glu Phe Ser Val Leu Gly Ser Asp Leu Arg Leu 450 455 460Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Lys Asn Leu Gly465 470 475 480Leu Thr Thr Val Thr Phe Trp Thr Ala Thr Leu Leu His Glu Phe Glu 485 490 495Trp Leu Thr Pro Ser Asp Glu Lys Thr Val Asp Leu Ser Glu Lys Leu 500 505 510Arg Leu Ser Cys Glu Met Ala Asn Pro Leu Ala Ala Lys Leu Arg Pro 515 520 525Arg Arg Ser Phe Ser Val 53044535PRTArabidopsis thaliana 44Met Arg Thr Glu Ile Glu Ser Leu Trp Val Phe Ala Leu Ala Ser Lys1 5 10 15Phe Asn Ile Tyr Met Gln Gln His Phe Ala Ser Leu Leu Val Ala Ile 20 25 30Ala Ile Thr Trp Phe Thr Ile Thr Ile Val Phe Trp Ser Thr Pro Gly 35 40 45Gly Pro Ala Trp Gly Lys Tyr Phe Phe Thr Arg Arg Phe Ile Ser Leu 50 55 60Asp Tyr Asn Arg Lys Tyr Lys Asn Leu Ile Pro Gly Pro Arg Gly Phe65 70 75 80Pro Leu Val Gly Ser Met Ser Leu Arg Ser Ser His Val Ala His Gln 85 90 95Arg Ile Ala Ser Val Ala Glu Met Ser Asn Ala Lys Arg Leu Met Ala 100 105 110Phe Ser Leu Gly Asp Thr Lys Val Val Val Thr Cys His Pro Ala Val 115 120 125Ala Lys Glu Ile Leu Asn Ser Ser Val Phe Ala Asp Arg Pro Val Asp 130 135 140Glu Thr Ala Tyr Gly Leu Met Phe Asn Arg Ala Met Gly Phe Ala Pro145 150 155 160Asn Gly Thr Tyr Trp Arg Thr Leu Arg Arg Leu Gly Ser Asn His Leu 165 170 175Phe Asn Pro Lys Gln Ile Lys Gln Ser Glu Asp Gln Arg Arg Val Ile 180 185 190Ala Thr Gln Met Val Asn Ala Phe Ala Arg Asn Pro Lys Ser Ala Cys 195 200 205Ala Val Arg Asp Leu Leu Lys Thr Ala Ser Leu Cys Asn Met Met Gly 210 215 220Leu Val Phe Gly Arg Glu Tyr Glu Leu Glu Ser Asn Asn Asn Leu Glu225 230 235 240Ser Glu Cys Leu Lys Gly Leu Val Glu Glu Gly Tyr Asp Leu Leu Gly 245 250 255Thr Leu Asn Trp Thr Asp His Leu Pro Trp Leu Ala Gly Leu Asp Phe 260 265 270Gln Gln Ile Arg Phe Arg Cys Ser Gln Leu Val Pro Lys Val Asn Leu 275 280 285Leu Leu Ser Arg Ile Ile His Glu Gln Arg Ala Ala Thr Gly Asn Phe 290 295 300Leu Asp Met Leu Leu Ser Leu Gln Gly Ser Glu Lys Leu Ser Glu Ser305 310 315 320Asp Met Val Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr 325 330 335Val Ala Val Leu Val Glu Trp Val Leu Ala Arg Ile Val Met His Pro 340 345 350Lys Val Gln Leu Thr Val His Asp Glu Leu Asp Arg Val Val Gly Arg 355 360 365Ser Arg Thr Val Asp Glu Ser Asp Leu Pro Ser Leu Thr Tyr Leu Thr 370 375 380Ala Met Ile Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu Leu385 390 395 400Ser Trp Ala Arg Leu Ser Ile Thr Asp Thr Ser Val Asp Gly Tyr His 405 410 415Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ala Arg 420 425 430Asp Pro His Val Trp Glu Asp Pro Leu Glu Phe Lys Pro Glu Arg Phe 435 440 445Val Ala Lys Glu Gly Glu Ala Glu Phe Ser Val Phe Gly Ser Asp Leu 450 455 460Arg Leu Ala Pro Phe Gly Ser Gly Lys Arg Val Cys Pro Gly Lys Asn465 470 475 480Leu Gly Leu Thr Thr Val Ser Phe Trp Val Ala Thr Leu Leu His Glu 485 490 495Phe Glu Trp Leu Pro Ser Val Glu Ala Asn Pro Pro Asp Leu Ser Glu 500 505 510Val Leu Arg Leu Ser Cys Glu Met Ala Cys Pro Leu Ile Val Asn Val 515 520 525Ser Ser Arg Arg Lys Ile Met 530 53545536PRTArabidopsis thaliana 45Met Glu Leu Met Asn Leu Ala Ser Lys Glu Thr Ser Tyr Trp Met Ile1 5 10 15Ala Leu Pro Ala Gly Phe Gly Ser Gln Asn Leu His Asp Val Ser Thr 20 25 30Leu Gly Tyr Leu Phe Leu Ala Val Val Phe Leu Ser Ile Val Thr Trp 35 40 45Ala Leu Ala Gly Gly Gly Gly Val Ala Trp Lys Asn Gly Arg Asn Arg 50 55 60Leu Gly Arg Val Ala Ile Pro Gly Pro Arg Gly Ile Pro Val Phe Gly65 70 75 80Ser Leu Phe Thr Leu Ser Arg Gly Leu Ala His Arg Thr Leu Ala Ala 85 90 95Met Ala Trp Ser Arg Ala Asn Thr Glu Ile Met Ala Phe Ser Leu Gly 100 105 110Ser Thr Pro Val Ile Val Ala Ser Glu Pro Asn Ile Ala Arg Glu Ile 115 120 125Leu Met Ser Pro His Phe Ala Asp Arg Pro Val Lys Gln Ser Ala Lys 130 135 140Ser Leu Met Phe Ser Arg Ala Ile Gly Phe Ala Pro Asn Gly Thr Tyr145 150 155 160Trp Arg Met Leu Arg Arg Ile Ala Ser Thr His Leu Phe Ala Pro Arg 165 170 175Arg Ile Leu Ala His Glu Ala Gly Arg Gln Leu Asp Cys Ala Glu Met 180 185 190Val Lys Ala Val Ser Val Glu Gln Asn Gly Ala Gly Ser Val Val Leu 195 200 205Arg Lys His Leu Gln Leu Ala Ala Leu Asn Asn Ile Met Gly Ser Val 210 215 220Phe Gly Arg Arg Tyr Asp Pro Leu Ala Gln Lys Glu Asp Leu Asp Glu225 230 235 240Leu Thr Ser Met Val Arg Glu Gly Phe Glu Leu Leu Gly Ala Phe Asn 245 250 255Trp Ser Asp Tyr Leu Pro Trp Leu Gly Tyr Phe Tyr Asp Ser Ile Arg 260 265 270Leu Asn Gln Arg Cys Ser Asp Leu Val Pro Arg Ile Arg Thr Leu Val 275 280 285Lys Lys Ile Ile Asp Glu His Arg Val Ser Asn Ser Glu Lys Lys Arg 290 295 300Asp Ile Gly Asp Phe Val Asp Val Leu Leu Ser Leu Asp Gly Asp Glu305 310 315 320Lys Leu Gln Glu Asp Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe 325 330 335Arg Gly Thr Asp Thr Thr Ala Leu Leu Thr Glu Trp Thr Met Ala Glu 340 345 350Leu Val Leu Asn Pro Asn Val Gln Thr Lys Leu Arg Asp Glu Ile Leu 355 360 365Thr Ala Val Gly Asp Gly Ala Asp Gly Asp Val Ala Asp Ala Asp Leu 370 375 380Ala Lys Leu Pro Tyr Leu Asn Ala Val Val Lys Glu Thr Leu Arg Leu385 390 395 400His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ser Thr Ser Asp 405 410 415Val Gln Leu Ser Asn Gly Met Val Ile Pro Lys Gly Thr Thr Ala Met 420 425 430Val Asn Met Trp Ala Ile Thr His Asp Gln Thr Val Trp Ser Asp Pro 435 440 445Leu Lys Phe Asp Pro Glu Arg Phe Thr Gly Asn Ala Asp Met Asp Ile 450 455 460Arg Gly Gly Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Val465 470 475 480Cys Pro Gly Lys Asn Met Gly Leu Ala Thr Val Thr Arg Trp Val Ala 485 490 495Glu Leu Val Arg Arg Phe Glu Trp Gly Gln Asp Gln Thr Glu Pro Val 500 505 510Asp Leu Gly Glu Val Leu Lys Leu Ser Cys Glu Met Glu His Pro Leu 515 520 525Arg Ala Val Val Thr Glu Ile Phe 530 53546517PRTArabidopsis thaliana 46Met Ser Pro Glu Ala Tyr Val Leu Phe Phe Asn Ser Phe Asn Leu Val1 5 10 15Thr Phe Glu Ala Phe Ala Ser Val Ser Leu Ile Ile Ala Thr Val Ala 20 25 30Phe Leu Leu Ser Pro Gly Gly Leu Ala Trp Ala Trp Thr Gly Ser Ser 35 40 45Lys Ser Arg Val Ser Ile Pro Gly Pro Ser Gly Ser Leu Ser Val Phe 50 55 60Ser Gly Ser Asn Pro His Arg Val Leu Ala Ala Leu Ala Lys Arg Phe65 70 75 80Lys Ala Ser Pro Leu Met Ala Phe Ser Val Gly Phe Ser Arg Phe Val 85 90 95Ile Ser Ser Glu Pro Glu Thr Ala Lys Glu Ile Leu Ser Ser Ser Ala 100 105 110Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Glu Leu Leu Phe His 115 120 125Arg Ala Met Gly Phe Ala Pro Tyr Gly Glu Tyr Trp Arg Asn Leu Arg 130 135 140Arg Ile Ser Ser Thr His Leu Phe Ser Pro Arg Arg Ile Ala Ser Phe145 150 155 160Glu Gly Val Arg Val Gly Ile Gly Met Lys Met Val Lys Lys Ile Lys 165 170 175Ser Leu Val Thr Ser Asp Ala Cys Gly Glu Val Glu Val Lys Lys Ile 180 185 190Val His Phe Gly Ser Leu Asn Asn Val Met Thr Thr Val Phe Gly Glu 195 200 205Ser Tyr Asp Phe Asp Glu Val Asn Gly Lys Gly Cys Phe Leu Glu Arg 210 215 220Leu Val Ser Glu Gly Tyr Glu Leu Leu Gly Ile Phe Asn Trp Ser Asp225 230 235 240His Phe Trp Phe Leu Arg Trp Phe Asp Phe Gln Gly Val Arg Lys Arg 245 250 255Cys Arg Ala Leu Val Ser Glu Val Asn Thr Phe Val Gly Gly Ile Ile 260 265 270Glu Lys His Lys Met Lys Lys Gly Asn Asn Leu Asn Gly Glu Glu Asn 275 280 285Asp Phe Val Asp Val Leu Leu Gly Leu Gln Lys Asp Glu Lys Leu Ser 290 295 300Asp Ser Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr305 310 315 320Asp Thr Val Ala Ile Leu Val Glu Trp Val Leu Ala Arg Met Val Leu 325 330 335His Gln Asp Ile Gln Asp Lys Leu Tyr Arg Glu Ile Ala Ser Ala Thr 340 345 350Ser Asn Asn Ile Arg Ser Leu Ser Asp Ser Asp Ile Pro Lys Leu Pro 355 360 365Tyr Leu Gln Ala Ile Val Lys Glu Thr Leu Arg Leu His Pro Pro Gly 370 375 380Pro Leu Leu Ser Trp Ala Arg Leu Ala Ile His Asp Val His Val Gly385 390 395 400Pro Asn Leu Val Pro Ala Gly Thr Ile Ala Met Val Asn Met Trp Ser 405 410 415Ile Thr His Asn Ala Lys Ile Trp Thr Asp Pro Glu Ala Phe Met Pro 420 425 430Glu Arg Phe Ile Ser Glu Asp Val Ser Ile Met Gly Ser Asp Leu Arg 435 440 445Leu Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Lys Ala Met 450 455 460Gly Leu Ala Thr Val His Leu Trp Ile Gly Gln Leu Ile Gln Asn Phe465 470 475 480Glu Trp Val Lys Gly Ser Cys Asp Val Glu Leu Ala Glu Val Leu Lys 485 490 495Leu Ser Met Glu Met Lys Asn Pro Leu Lys Cys Lys Ala Val Pro Arg 500 505 510Asn Val Gly Phe Ala 51547537PRTArabidopsis thaliana 47Met Thr Ile Asp Met Tyr Leu Ser Phe Ala Ser Arg Ser Gly Ser Ser1 5 10 15Pro Phe Pro Ser Leu Glu Leu Cys Leu Ser Ile Phe Leu Phe Ile Ser 20 25 30Leu Phe Val Phe Trp Leu Thr Pro Gly Gly Phe Ala Trp Ala Leu Tyr 35 40 45Lys Ala Arg Phe His Thr Arg Pro Glu Ser Lys Thr Gly Pro Ala Ile 50 55 60Pro Gly Pro Ser Gly Leu Pro Ile Phe Gly Leu Leu Leu Ala Phe Val65 70 75 80Asn Asn Ala Leu Thr His Arg Ile Leu Ala Asn Ile Ala Asp Thr Cys 85 90 95Lys Ala Lys Ala Leu Met Ala Phe Ser Val Gly Ser Thr Arg Phe Val 100 105 110Ile Thr Ser Glu Pro Glu Thr Ala Lys Glu Leu Leu Asn Ser Ser Ala 115 120

125Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Glu Leu Leu Phe Asp 130 135 140Arg Ala Met Gly Phe Ala Pro Phe Gly Asp Tyr Trp Arg Glu Leu Arg145 150 155 160Arg Ile Ser Ser Thr His Leu Phe Ser Pro Lys Arg Ile Phe Ser Ser 165 170 175Gly Glu Ser Arg Arg Lys Ile Gly Gln Asn Met Val Gly Glu Ile Lys 180 185 190Asn Ala Met Glu Cys Tyr Gly Glu Val His Ile Lys Lys Ile Leu His 195 200 205Phe Gly Ser Leu Asn Asn Val Met Ser Ser Val Phe Gly Lys Thr Tyr 210 215 220Asn Phe Asn Glu Gly Ile Val Tyr Ser Lys Glu Ser Asn Glu Leu Glu225 230 235 240His Leu Val Ser Glu Gly Tyr Glu Leu Leu Gly Ile Phe Asn Trp Ser 245 250 255Asp His Phe Pro Gly Met Arg Trp Leu Asp Leu Gln Gly Val Arg Arg 260 265 270Arg Cys Arg Ser Leu Val Gly Arg Val Asn Val Phe Val Gly Lys Ile 275 280 285Ile Asn Asp His Lys Ser Lys Arg Ser Leu Arg Asp Asn Pro Glu Glu 290 295 300Ser Thr Tyr Asp Asp Asp Phe Val Asp Val Leu Leu Gly Met His Gly305 310 315 320Asn Ser Lys Leu Ser Asp Ser Asp Met Ile Ala Val Leu Trp Glu Met 325 330 335Ile Phe Arg Gly Thr Asp Thr Val Ala Ile Leu Leu Glu Trp Ile Leu 340 345 350Ala Arg Met Val Leu His Pro Asp Ile Gln Ala Lys Ala Gln Ala Glu 355 360 365Ile Asp Cys Ile Val Gly Asp Ser Gly Arg Gln Val Thr Asp Ser Asp 370 375 380Leu Pro Lys Leu Pro Tyr Val Arg Ala Ile Val Lys Glu Thr Leu Arg385 390 395 400Met His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ser Ile His 405 410 415Asp Thr Gln Ile Gly Thr His Phe Ile Pro Ala Gly Thr Thr Ala Met 420 425 430Val Asn Met Trp Ala Ile Thr His Asp Glu Lys Val Trp Pro Glu Ala 435 440 445His Glu Tyr Lys Pro Glu Arg Phe Leu Gly Ala Gln Glu Ser Asn Asn 450 455 460Phe Pro Ile Met Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly465 470 475 480Arg Arg Val Cys Pro Gly Lys Ser Met Gly Leu Ala Thr Val Glu Leu 485 490 495Trp Leu Ala Gln Leu Leu Gly Ser Tyr Lys Trp Val Ser Cys Gly Glu 500 505 510Val Asp Leu Ser Glu Thr Leu Lys Leu Ser Leu Glu Met Lys Asn Thr 515 520 525Leu Val Cys Lys Ala Ile Pro Arg Gly 530 53548555PRTOryza sativa 48Met Ala Met Ala Thr Ala Thr Ala Ser Ser Cys Val Asp Ala Thr Trp1 5 10 15Trp Ala Tyr Ala Leu Pro Ala Leu Leu Gly Ala Asp Thr Leu Cys Ala 20 25 30His Pro Ala Leu Leu Ala Gly Ala Val Leu Leu Ala Phe Ala Thr Ala 35 40 45Ala Val Leu Ala Trp Ala Ala Ser Pro Gly Gly Pro Ala Trp Ala His 50 55 60Gly Arg Gly Arg Leu Gly Ala Thr Pro Ile Glu Gly Pro Arg Gly Leu65 70 75 80Pro Val Phe Gly Ser Ile Phe Ala Leu Ser Arg Gly Leu Pro His Arg 85 90 95Ala Leu Asp Ala Met Ser Arg Asp Ala Ala Ala Pro Arg Ala Arg Glu 100 105 110Leu Met Ala Phe Ser Val Gly Glu Thr Pro Ala Val Val Ser Ser Cys 115 120 125Pro Ala Thr Ala Arg Glu Val Leu Ala His Pro Ser Phe Ala Asp Arg 130 135 140Pro Leu Lys Arg Ser Ala Arg Glu Leu Leu Phe Ala Arg Ala Ile Gly145 150 155 160Phe Ala Pro Ser Gly Glu Tyr Trp Arg Leu Leu Arg Arg Ile Ala Ser 165 170 175Thr His Leu Phe Ser Pro Arg Arg Val Ala Ala His Glu Pro Gly Arg 180 185 190Gln Ala Asp Ala Thr Ala Met Leu Ser Ala Met Ala Ala Glu Gln Ser 195 200 205Ala Thr Gly Ala Val Val Leu Arg Pro His Leu Gln Ala Ala Ala Leu 210 215 220Asn Asn Ile Met Gly Ser Val Phe Gly Arg Arg Tyr Asp Val Ser Ser225 230 235 240Ser Ser Gly Ala Ala Ala Asp Glu Ala Glu Gln Leu Lys Ser Met Val 245 250 255Arg Glu Gly Phe Glu Leu Leu Gly Ala Phe Asn Trp Ser Asp His Leu 260 265 270Pro Trp Leu Ala His Leu Tyr Asp Pro Asn His Val Ala Arg Arg Cys 275 280 285Ala Ala Leu Val Pro Arg Val Gln Ala Phe Val Arg Gly Val Ile Arg 290 295 300Asp His Arg Leu Arg Arg Asp Ser Ser Ser Thr Ala Ala Asp Asn Ala305 310 315 320Asp Phe Val Asp Val Leu Leu Ser Leu Glu Ala His Glu Asn Leu Ala 325 330 335Glu Asp Asp Met Val Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr 340 345 350Asp Thr Thr Ala Leu Val Thr Glu Trp Cys Met Ala Glu Val Val Arg 355 360 365Asn Pro Ala Val Gln Ala Arg Leu Arg Ala Glu Val Asp Ala Ala Val 370 375 380Gly Gly Asp Gly Cys Pro Ser Asp Gly Asp Val Ala Arg Met Pro Tyr385 390 395 400Leu Gln Ala Val Val Lys Glu Thr Leu Arg Ala His Pro Pro Gly Pro 405 410 415Leu Leu Ser Trp Ala Arg Leu Ala Thr Ala Asp Val Gly Leu Ala Asn 420 425 430Gly Met Val Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala 435 440 445Ile Thr His Asp Gly Glu Val Trp Ala Asp Pro Glu Ala Phe Ala Pro 450 455 460Glu Arg Phe Ile Pro Ser Glu Gly Gly Ala Asp Val Asp Val Arg Asp465 470 475 480Gly Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro 485 490 495Gly Lys Asn Leu Gly Leu Ala Thr Val Ser Leu Trp Val Ala Arg Leu 500 505 510Val His Ala Phe Asp Trp Phe Leu Pro Asp Gly Ser Pro Pro Val Ser 515 520 525Leu Asp Glu Val Leu Lys Leu Ser Leu Glu Met Lys Thr Pro Leu Ala 530 535 540Ala Ala Ala Thr Pro Arg Arg Arg Arg Ala Ala545 550 55549547PRTZea mays 49Met Ala Met Ala Ser Ala Ala Cys Ser Cys Thr Asp Gly Thr Trp Trp1 5 10 15Val Tyr Ala Leu Pro Ala Leu Leu Gly Ser Asp Thr Leu Cys Ala His 20 25 30Pro Ala Leu Leu Ala Gly Leu Ile Phe Leu Ala Thr Val Ser Val Ala 35 40 45Leu Leu Ala Trp Ala Thr Ser Pro Gly Gly Pro Ala Trp Thr Asn Gly 50 55 60Arg Gly Ala Ser Ala Ser Leu Leu Ser Trp Asp Pro Val Val Cys Pro65 70 75 80Cys Ser Ala Ala Ser Ser Arg Cys Pro Gly Ala Ala Ala Pro Arg Pro 85 90 95Arg Arg Asp Gly Pro Arg Arg Arg Pro Arg Ala Lys Glu Leu Met Ala 100 105 110Phe Ser Val Gly Asp Thr Pro Ala Val Val Ser Ser Cys Pro Ala Thr 115 120 125Ala Arg Glu Val Leu Ala His Pro Ser Phe Ala Asp Arg Pro Val Lys 130 135 140Arg Ser Ala Arg Glu Leu Met Phe Ala Arg Ala Ile Gly Phe Ala Pro145 150 155 160Asn Gly Glu Tyr Trp Arg Arg Leu Arg Arg Val Ala Ser Thr His Leu 165 170 175Phe Ser Pro Arg Arg Val Ala Ser His Glu Pro Gly Arg Gln Gly Asp 180 185 190Ala Glu Ala Met Leu Arg Ser Ile Ala Ala Glu Gln Ser Ala Ser Gly 195 200 205Ala Val Ala Leu Arg Pro His Leu Gln Ala Ala Ala Leu Asn Asn Ile 210 215 220Met Gly Ser Val Phe Gly Thr Arg Tyr Asp Val Thr Ser Gly Ala Gly225 230 235 240Ala Ala Glu Ala Glu His Leu Lys Ser Met Val Arg Glu Gly Phe Glu 245 250 255Leu Leu Gly Ala Phe Asn Trp Ser Asp His Leu Pro Trp Leu Ala His 260 265 270Leu Tyr Asp Pro Ser Asn Val Thr Arg Arg Cys Ala Ala Leu Val Pro 275 280 285Arg Val Gln Thr Phe Val Arg Gly Val Ile Asp Glu His Arg Arg Arg 290 295 300Arg Gln Asn Ser Ala Ala Leu Asn Asp Asn Ala Asp Phe Val Asp Val305 310 315 320Leu Leu Ser Leu Glu Gly Asp Glu Lys Leu Gly Asp Asp Asp Met Val 325 330 335Ala Ile Leu Trp Glu Met Val Phe Arg Gly Thr Asp Thr Thr Ala Leu 340 345 350Leu Thr Glu Trp Cys Met Ala Glu Leu Val Arg His Pro Ala Val Gln 355 360 365Ala Arg Val Arg Ala Glu Val Asp Ala Ala Val Gly Ala Gly Gly Cys 370 375 380Pro Thr Asp Ala Asp Val Ala Arg Met Pro Tyr Leu Gln Ala Val Val385 390 395 400Lys Glu Thr Leu Arg Ala His Pro Pro Gly Pro Leu Leu Ser Trp Ala 405 410 415Arg Leu Ala Thr Ala Asp Val Pro Leu Cys Asn Gly Met Val Val Pro 420 425 430Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Thr His Asp Ala 435 440 445Ala Val Trp Ala Asp Pro Asp Ala Phe Ala Pro Glu Arg Phe Leu Pro 450 455 460Ser Glu Gly Gly Ala Asp Val Asp Val Arg Gly Val Asp Leu Arg Leu465 470 475 480Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Lys Asn Leu Gly 485 490 495Leu Thr Thr Val Gly Leu Trp Val Ala Arg Leu Val His Ala Phe Gln 500 505 510Trp Ala Leu Pro Asp Gly Ala Ala Ala Val Cys Leu Asp Glu Val Leu 515 520 525Lys Leu Ser Leu Glu Met Lys Thr Pro Leu Val Ala Ala Ala Ile Pro 530 535 540Arg Thr Ala54550426PRTPhalaenopsis sp. SM9108 50Met Ala Phe Ser Val Gly Leu Thr Arg Phe Ile Val Ser Ser His Pro1 5 10 15Lys Thr Ala Lys Glu Ile Leu Ser Ser Pro Ala Phe Ala Asp Arg Pro 20 25 30Ile Lys Glu Ser Ala Tyr Glu Leu Leu Phe Asn Arg Ala Met Gly Phe 35 40 45Ala Pro Phe Gly Asp Tyr Trp Arg Asn Leu Arg Arg Ile Ser Ser Thr 50 55 60Tyr Leu Phe Ser Pro Arg Arg Val Ser Ser Phe Glu Lys Gln Arg Ser65 70 75 80Glu Ile Gly Glu Gly Met Val Arg Asp Met Lys Arg Met Met Glu Arg 85 90 95Asn Gly Val Val Glu Val Arg Arg Met Leu His Tyr Gly Ser Leu Asn 100 105 110Asn Ile Met Leu Thr Val Phe Gly Lys Lys Phe Asp Phe Ala Lys Asp 115 120 125Glu Gly Leu Glu Leu Glu Leu Ile Leu Lys Glu Gly Tyr Glu Leu Leu 130 135 140Gly Ile Phe Asn Trp Gly Asp His Leu Pro Leu Leu Gly Trp Leu Asp145 150 155 160Leu Gln Gly Val Arg Arg Arg Cys Arg Thr Leu Val Ala Lys Val Asn 165 170 175Val Phe Val Lys Lys Ile Ile Asp Glu His Lys Arg Arg Ala Asn Gly 180 185 190Val Gly Ile Asp Glu Gly Glu Gly Glu Asp Phe Val Asp Val Leu Leu 195 200 205Gly Leu Glu Glu Lys Asp Arg Leu Ser Glu Ser Asp Met Val Ala Val 210 215 220Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala Ile Leu Leu225 230 235 240Glu Trp Thr Leu Ala Arg Met Val Leu His Pro Asp Ile Gln Ser Lys 245 250 255Ala Gln Val Glu Ile Asp Ser Val Val Asp Ser Ser Arg Pro Val Leu 260 265 270Asp Ser Asp Ile Gln Arg Leu Pro Tyr Leu Gln Ser Ile Val Lys Glu 275 280 285Thr Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu 290 295 300Ala Ile His Asp Val Pro Val Asp Gly His Met Ile Pro Ala Gly Thr305 310 315 320Thr Ala Met Val Asn Met Trp Ala Ile Thr His Asp Glu Cys Asn Trp 325 330 335Ala Glu Pro Asn Lys Phe Asn Pro Asp Arg Phe Ile Asp Glu Asp Val 340 345 350Asn Ile Leu Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Lys 355 360 365Arg Val Cys Pro Gly Lys Thr Met Ala Leu Ala Ala Val His Leu Trp 370 375 380Leu Ala Gln Leu Leu Lys Ser Phe Lys Leu Leu Pro Ser Arg Asn Gly385 390 395 400Val Asp Leu Ser Glu Cys Leu Lys Met Ser Leu Glu Met Lys Asn Pro 405 410 415Leu Val Cys Val Ala Val Pro Arg Phe Glu 420 42551523PRTGlycine max 51Met Thr Ser His Ile Asp Asp Asn Leu Trp Ile Ile Ala Leu Thr Ser1 5 10 15Lys Cys Thr Gln Glu Asn Leu Ala Trp Val Leu Leu Ile Met Gly Ser 20 25 30Leu Trp Leu Thr Met Thr Phe Tyr Tyr Trp Ser His Pro Gly Gly Pro 35 40 45Ala Trp Gly Lys Tyr Tyr Thr Tyr Ser Pro Pro Leu Ser Ile Ile Pro 50 55 60Gly Pro Lys Gly Phe Pro Leu Ile Gly Ser Met Gly Leu Met Thr Ser65 70 75 80Leu Ala His His Arg Ile Ala Ala Ala Ala Ala Thr Cys Arg Ala Lys 85 90 95Arg Leu Met Ala Phe Ser Leu Gly Asp Thr Arg Val Ile Val Thr Cys 100 105 110His Pro Asp Val Ala Lys Glu Ile Leu Asn Ser Ser Val Phe Ala Asp 115 120 125Arg Pro Val Lys Glu Ser Ala Tyr Ser Leu Met Phe Asn Arg Ala Ile 130 135 140Gly Phe Ala Ser Tyr Gly Val Tyr Trp Arg Ser Leu Arg Arg Ile Ala145 150 155 160Ser Asn His Leu Phe Cys Pro Arg Gln Ile Lys Ala Ser Glu Leu Gln 165 170 175Arg Ser Gln Ile Ala Ala Gln Met Val His Ile Leu Asn Asn Lys Arg 180 185 190His Arg Ser Leu Arg Val Arg Gln Val Leu Lys Lys Ala Ser Leu Ser 195 200 205Asn Met Met Cys Ser Val Phe Gly Gln Glu Tyr Lys Leu His Asp Pro 210 215 220Asn Ser Gly Met Glu Asp Leu Gly Ile Leu Val Asp Gln Gly Tyr Asp225 230 235 240Leu Leu Gly Leu Phe Asn Trp Ala Asp His Leu Pro Phe Leu Ala His 245 250 255Phe Asp Ala Gln Asn Ile Arg Phe Arg Cys Ser Asn Leu Val Pro Met 260 265 270Val Asn Arg Phe Val Gly Thr Ile Ile Ala Glu His Arg Ala Ser Lys 275 280 285Thr Glu Thr Asn Arg Asp Phe Val Asp Val Leu Leu Ser Leu Pro Glu 290 295 300Pro Asp Gln Leu Ser Asp Ser Asp Met Ile Ala Val Leu Trp Glu Met305 310 315 320Ile Phe Arg Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Ile Leu 325 330 335Ala Arg Met Ala Leu His Pro His Val Gln Ser Lys Val Gln Glu Glu 340 345 350Leu Asp Ala Val Val Gly Lys Ala Arg Ala Val Ala Glu Asp Asp Val 355 360 365Ala Val Met Thr Tyr Leu Pro Ala Val Val Lys Glu Val Leu Arg Leu 370 375 380His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ser Ile Asn Asp385 390 395 400Thr Thr Ile Asp Gly Tyr His Val Pro Ala Gly Thr Thr Ala Met Val 405 410 415Asn Thr Trp Ala Ile Cys Arg Asp Pro His Val Trp Lys Asp Pro Leu 420 425 430Glu Phe Met Pro Glu Arg Phe Val Thr Ala Gly Gly Asp Ala Glu Phe 435 440 445Ser Ile Leu Gly Ser Asp Pro Arg Leu Ala Pro Phe Gly Ser Gly Arg 450 455 460Arg Ala Cys Pro Gly Lys Thr Leu Gly Trp Ala Thr Val Asn Phe Trp465 470 475 480Val Ala Ser Leu Leu His Glu Phe Glu Trp Val Pro Ser Asp Glu Lys 485 490 495Gly Val Asp Leu Thr Glu Val Leu Lys Leu Ser Ser Glu Met Ala Asn 500 505 510Pro Leu Thr Val Lys Val Arg Pro Arg Arg Gly 515 52052553PRTPinus radiata

52Met Glu Asn Arg Arg Ser Ser Gly Gly Ser Gly Trp Trp Val Cys Val1 5 10 15Leu Pro Leu Phe Thr Lys Asp Gly Pro Ala Tyr Phe Leu His Ser Ser 20 25 30Ser Asp Asp Val Ser Ala Trp Arg Gln Trp Pro Leu Tyr Ile Ala Leu 35 40 45Leu Ile Val Ala Val Cys Ala Val Leu Val Ser Trp Leu Ser Pro Gly 50 55 60Gly Cys Ala Trp Ala Gly Arg His Lys Arg Gly Arg Val Ala Ile Pro65 70 75 80Gly Pro Lys Gly Trp Pro Ile Ile Gly Ser Leu Met Asp Met Ser Val 85 90 95Gly Leu Pro His Arg Lys Leu Glu Ser Leu Ala Arg Leu His Gly Ala 100 105 110Lys Gln Leu Met Ser Phe Ser Leu Gly Cys Thr Pro Ala Val Ile Thr 115 120 125Ser Asp Pro Glu Val Ala Arg Glu Leu Leu Thr Ser Pro His Phe Ala 130 135 140Asn Arg Pro Leu Lys Gln Ser Ala Gln Gln Leu Leu Phe Gly Arg Ala145 150 155 160Ile Gly Phe Ala Pro Asn Gly Gly Tyr Trp Arg Leu Leu Arg Arg Ile 165 170 175Ala Ser Ala His Leu Phe Ala Pro Arg Arg Ile Ala Ala His Glu Ala 180 185 190Gly Arg Gln Ala Asp Val Val Ala Met Leu Asp Asp Ile Gln Lys Glu 195 200 205Tyr His Ser Lys Gly Val Val Arg Val Arg Arg His Leu Gln Gly Ala 210 215 220Ala Leu Asn Asn Ile Met Gly Ser Val Phe Gly Arg Arg Phe Asp Met225 230 235 240Ser His Glu Asn Glu Glu Val Lys Lys Leu Arg Glu Met Val Asp Glu 245 250 255Gly Phe Gln Leu Leu Gly Ala Phe Asn Trp Ala Asp His Leu Pro Trp 260 265 270Leu Arg Pro Leu Asp Pro Leu Arg Ile His Ala Arg Cys Ala Arg Leu 275 280 285Val Pro Arg Val Thr Thr Phe Val Ser Asn Ile Ile Glu Gln His Arg 290 295 300Arg Glu Glu Gln Arg Arg Glu Ser Gly Asp Gln Cys Asp Phe Val Asp305 310 315 320Val Leu Leu Ser Leu Gln Gly Glu Asp Lys Leu Asp Glu Glu Asp Met 325 330 335Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Thr Ala 340 345 350Leu Leu Thr Glu Trp Thr Met Ala Glu Leu Val Leu His Pro Glu Ala 355 360 365Gln Lys Lys Ala Gln Ala Glu Leu Asp Ala Val Val Gly His Asp Arg 370 375 380Ser Val Lys Asp Ser Asp Ile Pro Lys Leu Pro Tyr Ile Gln Ala Val385 390 395 400Val Lys Glu Ala Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser Trp 405 410 415Ala Arg Leu Ser Thr Glu Asp Val Asn Met Gly Asp Gly Met Cys Val 420 425 430Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ser Ile Thr His Asp 435 440 445Pro Asn Ile Trp Glu Ser Pro Tyr Glu Phe Arg Pro Glu Arg Phe Val 450 455 460Val Phe Glu Gly Gly Glu Glu Val Asp Val Arg Gly Asn Asp Leu Arg465 470 475 480Leu Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Lys Ala Leu 485 490 495Gly Leu Ala Thr Val Asn Leu Trp Val Ala Lys Leu Leu His His Phe 500 505 510Glu Trp Leu Pro His Ala Glu His Pro Val Asp Leu Ser Glu Val Leu 515 520 525Lys Leu Ser Cys Glu Met Ala Arg Pro Leu His Cys Val Pro Val Thr 530 535 540Arg Val Pro Phe Ala Lys Phe Ser Asp545 55053523PRTGlycine max 53Met Thr Ser His Ile Asp Asp Asn Leu Trp Ile Ile Ala Leu Thr Ser1 5 10 15Lys Cys Thr Gln Glu Asn Leu Ala Trp Val Leu Leu Ile Met Gly Ser 20 25 30Leu Trp Leu Thr Met Thr Phe Tyr Tyr Trp Ser His Pro Gly Gly Pro 35 40 45Ala Trp Gly Lys Tyr Tyr Thr Tyr Ser Pro Pro Leu Ser Ile Ile Pro 50 55 60Gly Pro Lys Gly Phe Pro Leu Ile Gly Ser Met Gly Leu Met Ile Ser65 70 75 80Leu Ala His His Arg Ile Ala Ala Ala Ala Ala Thr Cys Arg Ala Lys 85 90 95Arg Leu Met Ala Phe Ser Leu Gly Asp Thr Arg Val Ile Val Thr Cys 100 105 110His Pro Asp Val Ala Lys Glu Ile Leu Asn Ser Ser Val Phe Ala Asp 115 120 125Arg Pro Val Lys Glu Ser Ala Tyr Ser Leu Met Phe Asn Arg Ala Ile 130 135 140Gly Phe Ala Ser Tyr Gly Val Tyr Trp Arg Ser Leu Arg Arg Ile Ala145 150 155 160Ser Asn His Phe Phe Cys Pro Arg Gln Ile Lys Ala Ser Glu Leu Gln 165 170 175Arg Ser Gln Ile Ala Ala Gln Met Val His Ile Leu Asn Asn Lys Arg 180 185 190His Arg Ser Leu Arg Val Arg Gln Val Leu Lys Lys Ala Ser Leu Ser 195 200 205Asn Met Met Cys Ser Val Phe Gly Gln Glu Tyr Lys Leu His Asp Pro 210 215 220Asn Ser Gly Met Glu Asp Leu Gly Ile Leu Val Asp Gln Gly Tyr Asp225 230 235 240Leu Leu Gly Leu Phe Asn Trp Ala Asp His Leu Pro Phe Leu Ala His 245 250 255Phe Asp Ala Gln Asn Ile Arg Phe Arg Cys Ser Asn Leu Val Pro Met 260 265 270Val Asn Arg Phe Val Gly Thr Ile Ile Ala Glu His Arg Ala Ser Lys 275 280 285Thr Glu Thr Asn Arg Asp Phe Val Asp Val Leu Leu Ser Leu Pro Glu 290 295 300Pro Asp Gln Leu Ser Asp Ser Asp Met Ile Ala Val Leu Trp Glu Met305 310 315 320Ile Phe Arg Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Ile Leu 325 330 335Ala Arg Met Ala Leu His Pro His Val Gln Ser Lys Val Gln Glu Glu 340 345 350Leu Asp Ala Val Val Gly Lys Ala Arg Ala Val Ala Glu Asp Asp Val 355 360 365Ala Val Met Thr Tyr Leu Pro Ala Val Val Lys Glu Val Leu Arg Leu 370 375 380His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ser Ile Asn Asp385 390 395 400Thr Thr Ile Asp Gly Tyr His Val Pro Ala Gly Thr Thr Ala Met Val 405 410 415Asn Met Trp Ala Ile Cys Arg Asp Pro His Val Trp Lys Asp Pro Leu 420 425 430Glu Phe Met Pro Glu Arg Phe Val Thr Ala Gly Gly Asp Ala Glu Phe 435 440 445Ser Ile Leu Gly Ser Asp Pro Arg Leu Ala Pro Phe Gly Ser Gly Arg 450 455 460Arg Ala Cys Pro Gly Lys Thr Leu Gly Trp Ala Thr Val Asn Phe Trp465 470 475 480Val Ala Ser Leu Leu His Glu Phe Glu Trp Val Pro Ser Asp Glu Lys 485 490 495Gly Val Asp Leu Thr Glu Val Leu Lys Leu Ser Ser Glu Met Ala Asn 500 505 510Pro Leu Thr Val Lys Val Arg Pro Arg Arg Gly 515 52054536PRTGlycine max 54Met Ser Thr His Ile Glu Ser Leu Trp Val Leu Ala Leu Ala Ser Lys1 5 10 15Cys Ile Gln Glu Asn Ile Ala Trp Ser Leu Leu Ile Ile Met Val Thr 20 25 30Leu Trp Leu Thr Met Thr Phe Phe Tyr Trp Ser His Pro Gly Gly Pro 35 40 45Ala Trp Gly Lys Tyr Tyr Tyr Phe Asn Tyr Trp Lys Lys Thr Thr Ser 50 55 60Thr Asn Thr Asn Ile Asn Leu Lys Met Ile Ile Pro Gly Pro Arg Gly65 70 75 80Tyr Pro Phe Ile Gly Ser Met Ser Leu Met Thr Ser Leu Ala His His 85 90 95Arg Ile Ala Ala Ala Gly Glu Ala Cys Asn Ala Thr Arg Leu Met Ala 100 105 110Phe Ser Met Gly Asp Thr Arg Ala Ile Val Thr Cys Asn Pro Asp Val 115 120 125Ala Lys Glu Ile Leu Asn Ser Ser Thr Phe Ala Asp Arg Pro Ile Lys 130 135 140Glu Ser Ala Tyr Ser Leu Met Phe Asn Arg Ala Ile Gly Phe Ala Pro145 150 155 160Tyr Gly Val Tyr Trp Arg Thr Leu Arg Arg Ile Ala Ala Thr His Leu 165 170 175Phe Cys Pro Lys Gln Ile Lys Ala Ser Glu Leu Gln Arg Ala Glu Ile 180 185 190Ala Ala Gln Met Thr Asn Ser Phe Arg Asn His Arg Cys Ser Gly Gly 195 200 205Phe Gly Ile Arg Ser Val Leu Lys Arg Ala Ser Leu Asn Asn Met Met 210 215 220Trp Ser Val Phe Gly Gln Lys Tyr Asn Leu Asp Glu Ile Asn Thr Ala225 230 235 240Met Asp Glu Leu Ser Met Leu Val Glu Gln Gly Tyr Asp Leu Leu Gly 245 250 255Thr Leu Asn Trp Gly Asp His Ile Pro Phe Leu Lys Asp Phe Asp Leu 260 265 270Gln Lys Ile Arg Phe Thr Cys Ser Lys Leu Val Pro Gln Val Asn Arg 275 280 285Phe Val Gly Ser Ile Ile Ala Asp His Gln Ala Asp Thr Thr Gln Thr 290 295 300Asn Arg Asp Phe Val His Val Leu Leu Ser Leu Gln Gly Pro Asp Lys305 310 315 320Leu Ser His Ser Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg 325 330 335Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Ile Leu Ala Arg Met 340 345 350Val Leu His Pro Glu Val Gln Arg Lys Val Gln Glu Glu Leu Asp Ala 355 360 365Val Val Arg Gly Gly Ala Leu Thr Glu Glu Val Val Ala Ala Thr Ala 370 375 380Tyr Leu Ala Ala Val Val Lys Glu Val Leu Arg Leu His Pro Pro Gly385 390 395 400Pro Leu Leu Ser Trp Ala Arg Leu Ala Ile Thr Asp Thr Thr Ile Asp 405 410 415Gly Tyr His Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala 420 425 430Ile Ala Arg Asp Pro Glu Val Trp Leu Asp Pro Leu Glu Phe Lys Pro 435 440 445Glu Arg Phe Met Gly Leu Glu Asn Glu Phe Ser Val Phe Gly Ser Asp 450 455 460Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg Arg Thr Cys Pro Gly Lys465 470 475 480Thr Leu Gly Leu Ser Thr Val Thr Phe Trp Val Ala Trp Leu Leu His 485 490 495Glu Phe Glu Trp Leu Pro Ser Asp Glu Ala Lys Val Asp Leu Thr Glu 500 505 510Val Leu Arg Leu Ser Cys Glu Met Ala Asn Pro Leu Ile Val Lys Val 515 520 525Arg Pro Arg His Gly Leu Ser Thr 530 53555551PRTOryza sativa 55Met Asp Met Asp Ser Ser Pro Ser Thr Gln Asp Cys Gly Gly Trp Leu1 5 10 15Leu Tyr Val Ser Leu Ala Ala Lys Cys Gly Gly Asp Pro Cys Arg Val 20 25 30Val Gly Phe Val Ala Val Ala Val Val Ala Phe Ala Val Thr Ser Leu 35 40 45Leu His Trp Leu Ser Pro Gly Gly Pro Ala Trp Gly Arg Tyr Trp Trp 50 55 60Asn Arg Arg Gly Gly Leu Gly Ile Ala Ala Ala Ile Pro Gly Pro Arg65 70 75 80Gly Leu Pro Val Leu Gly Ser Met Ser Leu Met Ala Gly Leu Ala His 85 90 95Arg Lys Leu Ala Ala Ala Ala Gly Gly Ser Pro Ala Arg Arg Arg Leu 100 105 110Met Ala Leu Ser Leu Gly Glu Thr Arg Val Val Val Thr Ala Asp Pro 115 120 125Gly Val Ala Arg Glu Leu Leu Ala Ser Ala Ala Phe Ala Asp Arg Pro 130 135 140Val Lys Glu Ser Ala Tyr Gly Met Leu Phe His Arg Ala Ile Gly Phe145 150 155 160Ala Pro Tyr Gly Thr Tyr Trp Arg Ala Leu Arg Arg Val Ala Ser Thr 165 170 175His Leu Phe Ser Pro Arg Gln Val Ser Ala Ser Ala Ala Gln Arg Ala 180 185 190Val Ile Ala Arg Gln Met Val Glu Ala Met Arg Ser Ala Ala Ala Ala 195 200 205Ala Ala Gly Gly Gly Val Ala Ala Arg Pro Phe Leu Lys Arg Ala Ser 210 215 220Leu His Asn Val Met Trp Ser Val Phe Gly Arg Lys Tyr Glu Leu Ala225 230 235 240Ala Pro Glu Ser Glu Glu Thr Ala Glu Leu Arg Ser Met Val Asp Glu 245 250 255Gly Tyr Asp Leu Leu Gly Gln Leu Asn Trp Ser Asp His Leu Pro Trp 260 265 270Leu Ala Pro Phe Asp Leu Lys Lys Thr Arg Ser Arg Cys Ser Ser Leu 275 280 285Val Pro Arg Val Asn Arg Phe Val Thr Arg Ile Ile Asp Glu His Arg 290 295 300Ala Arg Leu Ser Leu Ala Val Asp Ala Ala Val Asp Phe Thr Asp Val305 310 315 320Leu Leu Ser Leu His Gly Gly Asp Lys Leu Ser Asp Ala Asp Met Val 325 330 335Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala Val 340 345 350Leu Ile Glu Trp Val Ala Ala Arg Leu Val Leu His Gln Asp Val Gln 355 360 365Ala Arg Val His Asp Glu Leu Asp Arg Val Val Gly Ser Asp Arg Ala 370 375 380Val Thr Glu Ser Asp Ala Ser Lys Leu Val Tyr Leu Gln Ala Val Ile385 390 395 400Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu Leu Ser Trp Ala 405 410 415Arg Leu Ala Thr Ser Asp Val His Val Gly Gly Phe Leu Ile Pro Ser 420 425 430Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Thr His Asp Pro Ala 435 440 445Val Trp Pro Asp Pro Asn Glu Phe Lys Pro Glu Arg Phe Val Ala Gly 450 455 460Pro Ser Ser Asp Gln Ala Thr Glu Phe Pro Ile Met Gly Ser Asp Leu465 470 475 480Arg Leu Ala Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Lys Ser 485 490 495Leu Ala Ile Ala Thr Val Gly Phe Trp Val Ala Thr Leu Leu His Glu 500 505 510Phe Asp Trp Leu Pro Leu Ser Asp Lys Ser Arg Gly Val Asp Leu Ser 515 520 525Glu Val Leu Lys Leu Ser Cys Glu Met Ala Thr Pro Leu Glu Ala Arg 530 535 540Leu Arg Pro Arg Arg Lys Val545 55056536PRTBrachypodium distachyon 56Met Ala Thr Pro Glu Asp Cys Gly Ser Trp Leu Leu Tyr Leu Ser Leu1 5 10 15Ala Ala Lys Cys Gly Gly Asp Gly Asp His Pro Arg Arg Leu Ala Gly 20 25 30Leu Leu Ala Val Cys Ala Ala Ala Phe Leu Val Thr Cys Leu Leu His 35 40 45Trp Cys Phe Pro Gly Gly Pro Ala Trp Gly Arg Trp Trp Trp Thr Arg 50 55 60Arg Gly Leu Gly Arg Gly Pro Val Val Pro Gly Pro Arg Gly Leu Pro65 70 75 80Val Ile Gly Ser Met Trp Leu Met Thr Gly Leu Ala His Arg Lys Leu 85 90 95Ala Ala Glu Ala Ala Arg Leu Arg Gly Gly Gly Arg Arg Leu Met Ala 100 105 110Phe Ser Leu Gly Glu Thr Arg Val Val Val Ala Gly His Pro Asp Val 115 120 125Ala Arg Glu Ile Leu Thr Ser Pro Ala Phe Ala Asp Arg Pro Val Lys 130 135 140Glu Ser Ala Tyr Gly Leu Met Phe His Arg Ala Ile Gly Phe Ala Arg145 150 155 160His Gly Ala Tyr Trp Arg Ala Leu Arg Arg Val Ala Ser Thr His Leu 165 170 175Phe Ser Pro Trp Gln Val Ala Ala Ser Gly Ala Gln Arg Ala Val Ile 180 185 190Ala Arg Gln Met Val Ala Ala Leu Ala Gly Gly Ala Glu Val Arg Arg 195 200 205Val Leu Arg Arg Ala Ser Leu His Asn Val Met Trp Ser Val Phe Gly 210 215 220Arg Arg Tyr Asp Leu Glu Leu Asp Pro Gly Lys Glu Val Arg Glu Leu225 230 235 240Gly Gln Leu Val Asp Glu Gly Tyr Asp Leu Leu Gly Gln Leu Asn Trp 245 250 255Ser Asp His Leu Pro Trp Leu Ala Arg Phe Asp Leu Gln Gly Thr Arg 260 265 270Ala Arg Cys Ala Ser Leu Val Pro Arg Val Asn Arg Phe Val Gly Gly 275 280 285Ile Ile Asp Asp His Arg Val Lys Ala Pro Ser Ala Val Lys Asp Phe 290 295

300Thr Asp Val Leu Leu Gly Leu Gln Gly Gly Asp Arg Leu Ala Asp Ser305 310 315 320Asp Met Val Ala Val Leu Trp Glu Met Val Phe Arg Gly Thr Asp Thr 325 330 335Val Ala Val Leu Met Glu Trp Val Leu Ala Arg Leu Val Leu His Gln 340 345 350Asp Val Gln Ala Arg Val His Glu Glu Leu Asp Arg Val Val Gly Arg 355 360 365Asp Arg Ala Val Ala Glu Ser Asp Ala Ala Ser Leu Ala Tyr Leu His 370 375 380Ala Val Val Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu Leu385 390 395 400Ser Trp Ala Arg Leu Ala Thr Ser Asp Val His Val Asp Gly Phe Leu 405 410 415Ile Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Thr His 420 425 430Asp Gly Asp Val Trp Ala Glu Pro Met Glu Phe Arg Pro Glu Arg Phe 435 440 445Val Gly Pro Gly Ala Glu Glu Phe Ser Val Met Gly Ser Asp Leu Arg 450 455 460Leu Ala Pro Phe Gly Ala Gly Arg Arg Ser Cys Pro Gly Lys Ser Leu465 470 475 480Ala Met Ala Thr Val Ala Phe Trp Leu Ala Thr Leu Leu His Glu Phe 485 490 495Asp Leu Leu Pro Ser Ser Asp Pro Ala Arg Gly Val Gln Leu Ser Glu 500 505 510Thr Leu Arg Leu Ser Cys Glu Met Ala Thr Pro Leu Ala Leu Thr Pro 515 520 525Arg Ala Arg Arg Arg Pro Ala Val 530 53557542PRTGlycine max 57Met Thr Thr His Ile Asp Asn Leu Trp Val Leu Ala Leu Val Ser Lys1 5 10 15Cys Thr Gln Glu Asn Ile Ala Trp Ser Leu Leu Thr Ile Met Val Thr 20 25 30Leu Trp Leu Ser Met Thr Phe Phe Cys Trp Ser His Pro Gly Gly Pro 35 40 45Ala Trp Gly Lys Tyr Tyr Ser Phe His Tyr Trp Lys Lys Thr Thr Thr 50 55 60Thr Thr Thr Ser Thr Ser Asn Asn Thr Asn Ser Asn Asn Leu Lys Met65 70 75 80Ile Pro Gly Pro Lys Gly Tyr Pro Phe Ile Gly Ser Met Ser Leu Met 85 90 95Thr Ser Leu Ala His His Arg Ile Ala Ala Ala Ala Gln Ala Cys Lys 100 105 110Ala Thr Arg Leu Met Ala Phe Ser Met Gly Asp Thr Arg Val Ile Val 115 120 125Thr Cys His Pro His Val Ala Lys Glu Ile Leu Asn Ser Ser Val Phe 130 135 140Ala Asp Arg Pro Ile Lys Glu Ser Ala Tyr Ser Leu Met Phe Asn Arg145 150 155 160Ala Ile Gly Phe Ala Pro Tyr Gly Val Tyr Trp Arg Thr Leu Arg Arg 165 170 175Ile Ala Ala Thr His Leu Phe Cys Pro Lys Gln Ile Lys Ala Ser Glu 180 185 190Leu Gln Arg Ala Glu Ile Ala Ala Gln Met Thr His Ser Phe Arg Asn 195 200 205Arg Arg Gly Gly Phe Gly Ile Arg Ser Val Leu Lys Arg Ala Ser Leu 210 215 220Asn Asn Met Met Trp Ser Val Phe Gly Gln Arg Tyr Asp Leu Asp Glu225 230 235 240Thr Asn Thr Ser Val Asp Glu Leu Ser Arg Leu Val Glu Gln Gly Tyr 245 250 255Asp Leu Leu Gly Thr Leu Asn Trp Gly Asp His Ile Pro Phe Leu Lys 260 265 270Asp Phe Asp Leu Gln Lys Ile Arg Phe Thr Cys Ser Lys Leu Val Pro 275 280 285Gln Val Asn Arg Phe Val Gly Ser Ile Ile Ala Asp His Gln Thr Asp 290 295 300Thr Thr Gln Thr Asn Arg Asp Phe Val His Val Leu Leu Ser Leu Gln305 310 315 320Gly Pro Asp Lys Leu Ser His Ser Asp Met Ile Ala Val Leu Trp Glu 325 330 335Met Ile Phe Arg Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Ile 340 345 350Met Ala Arg Met Val Leu His Pro Glu Val Gln Arg Arg Val Gln Glu 355 360 365Glu Leu Asp Ala Val Val Gly Gly Gly Ala Arg Ala Leu Lys Glu Glu 370 375 380Asp Val Ala Ala Thr Ala Tyr Leu Leu Ala Val Val Lys Glu Val Leu385 390 395 400Arg Leu His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Ile 405 410 415Thr Asp Thr Thr Ile Asp Gly Tyr Asn Val Pro Ala Gly Thr Thr Ala 420 425 430Met Val Asn Met Trp Ala Ile Gly Arg Asp Pro Glu Val Trp Leu Asp 435 440 445Pro Leu Asp Phe Lys Pro Glu Arg Phe Met Gly Leu Glu Ala Glu Phe 450 455 460Ser Val Leu Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg465 470 475 480Arg Thr Cys Pro Gly Lys Thr Leu Gly Leu Ser Thr Val Thr Phe Trp 485 490 495Val Ala Arg Leu Leu His Glu Phe Glu Trp Leu Pro Ser Asp Glu Gly 500 505 510Lys Val Asp Leu Thr Glu Val Leu Arg Leu Ser Cys Glu Met Ala Asn 515 520 525Pro Leu Tyr Val Lys Val Arg Pro Arg Arg Gly Leu Ser Thr 530 535 54058553PRTOryza sativa 58Met Ala Thr Pro Glu Asp Thr Gly Ser Trp Leu Leu Tyr Leu Ser Leu1 5 10 15Ala Ala Lys Cys Ser Gly Asp Gly Asp Gly Gln Pro His Arg Leu Leu 20 25 30Gly Phe Val Val Val Cys Ala Val Ala Gly Leu Val Thr Cys Leu Leu 35 40 45His Trp Ser Phe Pro Gly Gly Pro Ala Trp Gly Arg Trp Trp Trp Thr 50 55 60Arg Arg Arg Arg Arg Gly Ser Pro Cys Gly Val Ala Ala Val Pro Gly65 70 75 80Leu Arg Gly Leu Pro Val Ile Gly Ser Met Trp Leu Met Thr Gly Leu 85 90 95Ala His Arg Lys Leu Ala Ala Ala Ala Glu Ala Ala Gly Ala Gly Arg 100 105 110Leu Met Ala Leu Ser Leu Gly Glu Thr Arg Val Val Val Ala Ala His 115 120 125Pro Asp Val Ala Arg Glu Ile Leu His Gly Ala Ala Phe Ala Asp Arg 130 135 140Pro Val Lys Glu Ser Ala Tyr Gly Leu Leu Phe His Arg Ala Ile Gly145 150 155 160Phe Ala Pro His Gly Ala Tyr Trp Arg Ala Leu Arg Arg Val Ala Ser 165 170 175Thr His Leu Phe Ser Pro Trp Gln Val Ala Ala Ser Ala Pro Gln Arg 180 185 190Ala Val Ile Ala Arg Gln Met Val Arg Ala Ile Lys Leu Gln Gln Arg 195 200 205Ser Arg Ser Gly Asp Ser Ala Ala Gly Ala Ala Val Glu Val Arg Arg 210 215 220Val Leu Arg Arg Ala Ser Leu His Asn Val Met Trp Ser Val Phe Gly225 230 235 240Arg Arg Tyr Glu Leu Gln Leu Asp Pro Gly Lys Glu Ser Asp Glu Val 245 250 255Arg Glu Leu Arg Ala Leu Val Asp Glu Gly Tyr Asp Leu Leu Gly Gln 260 265 270Leu Asn Trp Ser Asp His Leu Pro Trp Leu Ala Arg Phe Asp Leu Gln 275 280 285Ser Thr Arg Ala Arg Cys Ser Arg Leu Val Pro Arg Val Asn Arg Phe 290 295 300Val Thr Arg Ile Ile Asp Glu His Arg Ser Ser Ala Pro Val Ala Ala305 310 315 320Ala Ile Asp Phe Thr Asp Val Leu Leu Ser Leu Gln Gly Ser Asp Lys 325 330 335Leu Ala Asp Ser Asp Met Val Ala Val Leu Trp Glu Met Val Phe Arg 340 345 350Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Val Leu Ala Arg Leu 355 360 365Val Leu Gln Gln Asp Val Gln Ala Arg Val His Asp Glu Leu Gly Arg 370 375 380Val Val Gly Leu Asp Arg Asp Val Thr Glu Ser Asp Thr Ala Ser Leu385 390 395 400Val Tyr Leu His Ala Val Ile Lys Glu Thr Leu Arg Leu His Pro Pro 405 410 415Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Thr Ser Asp Val His Val 420 425 430Asp Gly Tyr Leu Ile Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp 435 440 445Ala Ile Ala His Asp Pro Asp Val Trp Ala Glu Pro Met Glu Phe Arg 450 455 460Pro Glu Arg Phe Ile Gly Lys Ala Ala Glu Phe Ser Val Met Gly Ser465 470 475 480Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly 485 490 495Lys Ser Leu Ala Met Ala Thr Val Ala Phe Trp Leu Ala Thr Leu Leu 500 505 510His Glu Phe Ala Leu Leu Pro Ser Pro Asp Pro Ala His Gly Val Asp 515 520 525Leu Ser Glu Val Leu Arg Leu Ser Cys Glu Met Ala Thr Pro Leu Ala 530 535 540Val Thr Ala Trp Pro Arg Arg Val Val545 55059516PRTGlycine max 59Met Ser Pro Asp Phe Thr Leu Leu Phe Phe Pro Glu Leu Met Gln Ser1 5 10 15Pro Met Ile Thr Phe Gln Ala Thr Leu Cys Val Leu Leu Phe Thr Leu 20 25 30Met Phe Thr Leu Leu Phe Thr Pro Gly Gly Leu Pro Trp Ala Trp Ala 35 40 45Arg Pro Arg Pro Ile Ile Pro Gly Pro Val Thr Ala Leu Leu Gly Ile 50 55 60Phe Thr Gly Ser Thr Pro His Arg Ala Leu Ser Lys Leu Ala Arg Asn65 70 75 80Tyr His Ala Glu Lys Leu Met Ala Phe Ser Ile Gly Leu Thr Arg Phe 85 90 95Val Ile Ser Ser Glu Pro Glu Thr Ala Lys Glu Ile Leu Gly Ser Pro 100 105 110Ser Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Glu Leu Leu Phe 115 120 125His Arg Ala Met Gly Phe Ala Pro Tyr Gly Glu Tyr Trp Arg Asn Leu 130 135 140Arg Arg Ile Ser Ala Leu His Leu Phe Ser Pro Lys Arg Ile Thr Gly145 150 155 160Ser Glu Ser Phe Arg Ser Glu Val Gly Leu Lys Met Val Glu Gln Val 165 170 175Lys Lys Thr Met Ser Glu Asn Gln His Val Glu Val Lys Lys Ile Leu 180 185 190His Phe Ser Ser Leu Asn Asn Val Met Met Thr Val Phe Gly Lys Ser 195 200 205Tyr Glu Phe Tyr Glu Gly Glu Gly Leu Glu Leu Glu Gly Leu Val Ser 210 215 220Glu Gly Tyr Glu Leu Leu Gly Val Phe Asn Trp Ser Asp His Phe Pro225 230 235 240Val Leu Gly Trp Leu Asp Leu Gln Gly Val Arg Lys Arg Cys Arg Cys 245 250 255Leu Val Glu Lys Val Asn Val Phe Val Gly Gly Val Ile Lys Glu His 260 265 270Arg Val Lys Arg Glu Arg Gly Glu Cys Val Lys Asp Glu Gly Thr Gly 275 280 285Asp Phe Val Asp Val Leu Leu Asp Leu Glu Lys Glu Asn Arg Leu Ser 290 295 300Glu Ala Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr305 310 315 320Asp Thr Val Ala Ile Leu Leu Glu Trp Thr Leu Ala Arg Met Val Leu 325 330 335His Pro Glu Ile Gln Ala Lys Ala Gln Arg Glu Ile Asp Phe Val Cys 340 345 350Gly Ser Ser Arg Pro Val Ser Glu Ala Asp Ile Pro Asn Leu Arg Tyr 355 360 365Leu Gln Cys Ile Val Lys Glu Thr Leu Arg Val His Pro Pro Gly Pro 370 375 380Leu Leu Ser Trp Ala Arg Leu Ala Val His Asp Val Thr Val Gly Gly385 390 395 400Lys His Val Ile Pro Lys Gly Thr Thr Ala Met Val Asn Met Trp Ala 405 410 415Ile Thr His Asp Glu Arg Val Trp Ala Glu Pro Glu Lys Phe Arg Pro 420 425 430Glu Arg Phe Val Glu Glu Asp Val Ser Ile Met Gly Ser Asp Leu Arg 435 440 445Leu Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Lys Ala Leu 450 455 460Gly Leu Ala Ser Val His Leu Trp Leu Ala Gln Leu Leu Gln Asn Phe465 470 475 480His Trp Val Ser Ser Asp Gly Val Ser Val Glu Leu Asp Glu Phe Leu 485 490 495Lys Leu Ser Met Glu Met Lys Lys Pro Leu Ser Cys Lys Ala Val Pro 500 505 510Arg Val Ser Val 51560516PRTGlycine max 60Met Ser Pro Asp Phe Thr Leu Leu Phe Ser Pro Glu Leu Met Gln Ser1 5 10 15Pro Ile Ile Thr Phe Gln Ala Thr Phe Cys Val Leu Leu Phe Thr Leu 20 25 30Met Phe Thr Pro Phe Phe Thr Pro Gly Gly Leu Pro Trp Ala Trp Ala 35 40 45Arg Pro Arg Thr Ile Ile Pro Gly Pro Val Thr Ala Leu Leu Gly Val 50 55 60Phe Thr Gly Ser Thr Pro His Ser Ala Leu Ser Lys Leu Ala Arg Thr65 70 75 80Tyr His Ala Glu Lys Leu Met Ala Phe Ser Ile Gly Leu Thr Arg Phe 85 90 95Val Ile Ser Ser Glu Pro Glu Thr Ala Lys Glu Ile Leu Gly Ser Pro 100 105 110Gly Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Glu Leu Leu Phe 115 120 125His Arg Ala Met Gly Phe Ala Pro Tyr Gly Glu Tyr Trp Arg Asn Leu 130 135 140Arg Arg Ile Ser Ala Leu His Leu Phe Ser Pro Lys Arg Ile Thr Ser145 150 155 160Ser Glu Ser Phe Arg Ser Lys Val Gly Leu Lys Met Val Glu Gln Val 165 170 175Lys Lys Thr Met Ser Glu Asn Gln His Val Glu Val Lys Lys Ile Leu 180 185 190His Phe Ser Ser Leu Asn Asn Val Met Met Thr Val Phe Gly Lys Cys 195 200 205Tyr Glu Phe Tyr Glu Gly Glu Gly Leu Glu Leu Glu Gly Leu Val Ser 210 215 220Glu Gly Tyr Glu Leu Leu Gly Val Phe Asn Trp Ser Asp His Phe Pro225 230 235 240Val Leu Gly Trp Leu Asp Leu Gln Gly Val Arg Lys Arg Cys Arg Cys 245 250 255Leu Val Glu Lys Val Asn Val Phe Val Gly Gly Val Ile Lys Glu His 260 265 270Arg Val Lys Arg Glu Arg Gly Asp Cys Val Lys Asp Glu Gly Ala Glu 275 280 285Asp Phe Val Asp Val Leu Leu Asp Leu Glu Lys Glu Asn Arg Leu Ser 290 295 300Glu Ala Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr305 310 315 320Asp Thr Val Ala Ile Leu Leu Glu Trp Ile Leu Ala Arg Met Val Leu 325 330 335His Pro Glu Ile Gln Ala Lys Ala Gln Arg Glu Ile Asp Phe Val Cys 340 345 350Gly Ser Ser Arg Leu Val Ser Glu Ala Asp Ile Pro Asn Leu Arg Tyr 355 360 365Leu Gln Cys Ile Val Lys Glu Thr Leu Arg Val His Pro Pro Gly Pro 370 375 380Leu Leu Ser Trp Ala Arg Leu Ala Val His Asp Val Thr Val Gly Gly385 390 395 400Lys His Val Ile Pro Lys Gly Thr Thr Ala Met Val Asn Met Trp Ala 405 410 415Ile Thr His Asp Glu Arg Val Trp Ala Glu Pro Glu Lys Phe Arg Pro 420 425 430Glu Arg Phe Val Glu Glu Asp Val Ser Ile Met Gly Ser Asp Leu Arg 435 440 445Leu Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Lys Ala Leu 450 455 460Gly Leu Ala Ser Val His Leu Trp Leu Ala Gln Leu Leu Gln Asn Phe465 470 475 480His Trp Val Ser Ser Asp Gly Val Ser Val Glu Leu Asp Glu Phe Leu 485 490 495Lys Leu Ser Met Glu Met Lys Lys Pro Leu Ser Cys Lys Ala Val Pro 500 505 510Arg Val Ser Val 51561543PRTZea mays 61Met Ala Pro Pro Thr Glu Asp Cys Gly Trp Leu Leu Tyr Leu Ser Leu1 5 10 15Ala Ala Lys Cys Gly Asp Pro Gln Arg Leu Leu Gly Phe Ala Ala Val 20 25 30Phe Val Ala Ala Cys Val Val Thr Ser Leu Leu His Trp Ala Ser Pro 35 40 45Gly Gly Pro Ala Trp Gly Trp Tyr Trp Trp Thr Arg Arg Ala Gly Leu 50 55 60Gly Ile Val Arg Ala Ala Ile Pro Gly Pro Arg Gly Leu Pro Val Val65 70 75 80Gly Ser Met Gly Leu Met Thr Gly Leu Ala His Arg Lys Leu Ser Ala 85 90 95Ala Ala Glu Arg Gln Ala Ser Arg Arg Arg Leu Met Ala

Phe Ser Leu 100 105 110Gly Glu Thr Arg Val Val Val Thr Ala Asp Pro Asp Val Ala Arg Glu 115 120 125Leu Leu Ala Ser Ala Ala Phe Ala Asp Arg Pro Val Lys Glu Ser Ala 130 135 140Tyr Gly Leu Leu Phe His Arg Ala Ile Gly Phe Ala Pro His Gly Ala145 150 155 160Tyr Trp Arg Ala Leu Arg Arg Val Ala Ser Ala His Leu Phe Ser Pro 165 170 175Arg Gln Ile Ala Ala Ser Ala Ala Gln Arg Ala Ala Ile Ala Arg Gln 180 185 190Met Val Asp Ala Thr Thr Thr Ala Ala Ala His Ala Pro Val Val Val 195 200 205Ala Arg Arg Phe Leu Lys Arg Ala Ser Leu His Asn Val Met Trp Ser 210 215 220Val Phe Gly Arg Arg Tyr Asp Leu Met Ala Asp Ser Arg Glu Ala Glu225 230 235 240Glu Leu Lys Ala Leu Val Asp Glu Gly Tyr Asp Leu Leu Gly Gln Leu 245 250 255Asn Trp Ser Asp His Leu Pro Trp Leu Ala Arg Phe Asp Leu Gln Lys 260 265 270Thr Arg Ala Arg Cys Cys Ala Leu Val Pro Arg Val Asn Arg Phe Val 275 280 285Gly Asn Ile Ile Gly Glu His Arg Ala Arg Leu Gly Arg Gly Val Asp 290 295 300Thr Ala Val Met Asp Phe Thr Asp Val Leu Leu Ser Leu Gln Gly Asp305 310 315 320Asp Lys Leu Ser Asp Ala Asp Met Ile Ala Val Leu Trp Glu Met Ile 325 330 335Phe Arg Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Val Leu Ala 340 345 350Arg Leu Val Leu His Gln Asp Val Gln Ser Lys Val Gln Glu Glu Leu 355 360 365Asp Arg Val Val Gly Leu Gly Gln Ala Val Thr Glu Ser Asp Thr Ala 370 375 380Ser Leu Pro Tyr Leu Gln Ala Val Ile Lys Glu Val Leu Arg Leu His385 390 395 400Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Thr Ser Asp Val 405 410 415His Val Gly Gly Tyr Leu Val Pro Ala Gly Thr Thr Ala Met Val Asn 420 425 430Met Trp Ala Ile Thr His Asp Pro Ser Leu Trp Pro Glu Pro Met Glu 435 440 445Phe Arg Pro Glu Arg Phe Met Gly Pro Ala Ala Glu Asp Val Pro Ile 450 455 460Met Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg Arg Ser465 470 475 480Cys Pro Gly Lys Ser Leu Ala Val Ala Thr Val Gly Phe Trp Val Ala 485 490 495Thr Leu Leu Tyr Glu Phe Lys Trp Leu Pro Pro Ser Asp Glu Pro Arg 500 505 510Gly Gly Gly Val Asp Leu Ser Glu Val Leu Arg Leu Ser Cys Glu Met 515 520 525Ala Ala Pro Leu Glu Ala Arg Val Val Pro Arg His Ala Val Cys 530 535 54062551PRTSorghum bicolor 62Met Ala Val Ala Ala Thr Pro Asp Asp Cys Gly Ser Trp Leu Leu Tyr1 5 10 15Leu Ser Leu Ala Ala Lys Cys Ala Gly Gly Asp Gln Pro His Arg Leu 20 25 30Ala Gly Phe Leu Ala Val Cys Ala Val Ala Phe Val Val Thr Cys Leu 35 40 45Leu His Trp Cys Phe Pro Gly Gly Pro Ala Trp Gly Arg Trp Trp Trp 50 55 60Thr Thr Gln Ala Arg Arg Val Ala Ala Ala Ala Val Pro Gly Pro Arg65 70 75 80Gly Leu Pro Val Val Gly Ser Met Trp Leu Met Thr Gly Leu Ala His 85 90 95Arg Lys Leu Ala Ala Ala Ala Asp Ser Leu Arg Ala Arg Arg Leu Met 100 105 110Ala Phe Ser Leu Gly Gly Thr Arg Val Val Val Ala Ala His Pro Asp 115 120 125Val Ala Arg Glu Ile Leu Asn Ser Pro Ala Phe Ala Asp Arg Pro Ile 130 135 140Lys Glu Ser Ala Tyr Gly Leu Leu Phe His Arg Ala Ile Gly Phe Ala145 150 155 160Pro Tyr Gly Ala Tyr Trp Arg Ala Leu Arg Arg Val Ala Ser Thr His 165 170 175Leu Phe Ser Pro Trp Gln Val Ala Ala Ser Ala Ala Gln Arg Ala Val 180 185 190Ile Ala Arg Gln Met Val Ala Ala Met Lys Gln Glu Leu Ser Ser Ser 195 200 205Ser Ser Ala Ser Ala Gly Phe Glu Val Arg Arg Val Leu Arg Arg Gly 210 215 220Ser Leu His Asn Val Met Trp Ser Val Phe Gly Arg Arg Tyr Asp Leu225 230 235 240Glu Leu Asp Pro Ala Lys Glu Ser Pro Glu Thr Arg Glu Leu Arg Ser 245 250 255Leu Val Asp Glu Gly Tyr Asp Leu Leu Gly Gln Leu Asn Trp Ser Asp 260 265 270His Leu Pro Trp Leu Ala Arg Phe Asp Leu Gln Ser Thr Arg Ser Arg 275 280 285Cys Asp Arg Leu Val Pro Leu Val Asn Arg Phe Val Gly Gly Ile Ile 290 295 300Asp Ala His Arg Ala Arg Asn Asp Leu Arg Ser Ala Pro Pro His Ala305 310 315 320Val Met Asp Phe Thr Asp Val Leu Leu Ser Leu Pro Ala Asp Asp Arg 325 330 335Leu Thr Asp Ser Asp Met Ile Ala Val Leu Trp Glu Met Val Phe Arg 340 345 350Gly Thr Asp Thr Val Ala Val Leu Ile Glu Trp Val Leu Ala Arg Leu 355 360 365Val Leu His Pro Asp Val Gln Ala Arg Val His Asp Glu Leu Asp Arg 370 375 380Val Val Gly Arg Asp Arg Ala Val Thr Glu Ser Asp Ser Gly Ser Leu385 390 395 400Val Tyr Leu His Ala Val Ile Lys Glu Val Leu Arg Met His Pro Pro 405 410 415Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Thr Ser Asp Val Gln Val 420 425 430Asp Gly His Leu Ile Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp 435 440 445Ala Ile Thr His Asp Pro Asp Val Trp Ala Glu Pro Ala Glu Phe Gln 450 455 460Pro Glu Arg Phe Met Gly Ser Thr Thr Gly Gly Glu Phe Pro Ile Met465 470 475 480Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Ser Cys 485 490 495Pro Gly Lys Ser Leu Ala Met Ala Thr Val Ala Leu Trp Leu Ala Thr 500 505 510Leu Leu His Glu Phe Glu Leu Leu Pro Ala Arg Gly Val Tyr Leu Ser 515 520 525Glu Val Leu Lys Leu Ser Cys Glu Met Ala Val Pro Leu Ala Val Thr 530 535 540Ala Arg Pro Arg Gln Ala Val545 55063528PRTGlycine max 63Met Ser Ser Ser Glu Leu Ser Ser Phe Phe Leu Leu Arg Leu Ser Asp1 5 10 15Ile Leu Ser Phe Asp Val Leu Leu Gly Val Met Phe Leu Val Ala Val 20 25 30Phe Gly Tyr Trp Leu Val Pro Gly Gly Leu Ala Trp Ala Phe Ser Lys 35 40 45Phe Lys Pro Ala Ile Pro Gly Pro Ser Gly Tyr Pro Val Val Gly Leu 50 55 60Val Trp Ala Phe Ile Gly Pro Leu Thr His Arg Val Leu Ala Lys Leu65 70 75 80Ala Glu Thr Phe Asp Ala Lys Pro Leu Met Ala Phe Ser Val Gly Phe 85 90 95Thr Arg Phe Ile Ile Ser Ser His Pro Asp Thr Ala Lys Glu Ile Leu 100 105 110Asn Ser Ser Ala Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Glu 115 120 125Leu Leu Phe His Arg Ala Met Gly Phe Ala Pro Tyr Gly Glu Tyr Trp 130 135 140Arg Asn Leu Arg Arg Ile Ser Ala Thr His Met Phe Ser Pro Arg Arg145 150 155 160Ile Ala Ala Gln Gly Val Phe Arg Ala Arg Ile Gly Ala Gln Met Val 165 170 175Arg Asp Ile Val Gly Leu Met Gly Arg Asp Gly Val Val Glu Val Arg 180 185 190Lys Val Leu His Phe Gly Ser Leu Asn Asn Val Met Lys Ser Val Phe 195 200 205Gly Arg Ser Tyr Val Phe Gly Glu Gly Gly Asp Gly Cys Glu Leu Glu 210 215 220Gly Leu Val Ser Glu Gly Tyr His Leu Leu Gly Val Phe Asn Trp Ser225 230 235 240Asp His Phe Pro Leu Leu Gly Trp Leu Asp Leu Gln Gly Val Arg Lys 245 250 255Ser Cys Arg Ser Leu Val Asp Arg Val Asn Val Tyr Val Gly Lys Ile 260 265 270Ile Leu Glu His Arg Val Lys Arg Val Ala Gln Gly Glu Asp Asn Lys 275 280 285Ala Ile Asp Thr Asp Ser Ser Gly Asp Phe Val Asp Val Leu Leu Asp 290 295 300Leu Glu Lys Glu Asn Arg Leu Asn His Ser Asp Met Val Ala Val Leu305 310 315 320Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala Ile Leu Leu Glu 325 330 335Trp Ile Leu Ala Arg Met Val Leu His Pro Glu Ile Gln Ala Lys Ala 340 345 350Gln Ser Glu Ile Asp Ser Val Val Gly Ser Gly Arg Ser Val Ser Asp 355 360 365Asp Asp Leu Pro Asn Leu Pro Tyr Val Arg Ala Ile Val Lys Glu Thr 370 375 380Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ser385 390 395 400Ile His Asp Thr Gln Ile Gly Asn His Phe Val Pro Ala Gly Thr Thr 405 410 415Ala Met Val Asn Met Trp Ala Ile Thr His Asp Gln Glu Val Trp Tyr 420 425 430Glu Pro Lys Gln Phe Lys Pro Glu Arg Phe Leu Lys Asp Glu Asp Val 435 440 445Pro Ile Met Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg 450 455 460Arg Val Cys Pro Gly Lys Ala Met Gly Leu Ala Thr Val Glu Leu Trp465 470 475 480Leu Ala Met Phe Leu Gln Lys Phe Lys Trp Met Pro Cys Asp Asp Ser 485 490 495Gly Val Asp Leu Ser Glu Cys Leu Lys Leu Ser Met Glu Met Lys His 500 505 510Ser Leu Lys Thr Lys Val Val Ala Arg Pro Val Val Ser Leu Ala Met 515 520 52564557PRTZea mays 64Met Ala Ala Pro Pro Thr Glu Asp Cys Gly Trp Leu Leu Tyr Leu Ser1 5 10 15Leu Ala Ala Lys Cys Gly Asp Pro Ser Arg Leu Leu Gly Leu Ala Ala 20 25 30Val Phe Val Gly Ala Cys Val Val Thr Ser Leu Leu His Trp Ala Cys 35 40 45Pro Gly Gly Pro Ala Trp Gly Arg Tyr Trp Trp Thr Arg Arg Gly Gly 50 55 60Leu Gly Ile Val Arg Ala Ala Ile Pro Gly Pro Arg Gly Leu Pro Val65 70 75 80Val Gly Ser Met Gly Leu Met Thr Gly Leu Ala His Arg Lys Leu Ala 85 90 95Ala Ala Ala Ala Ala Ala Gly Gly Gln Gly Ser Ser Arg Arg Arg Arg 100 105 110Leu Met Ala Leu Ser Leu Gly Glu Thr Arg Ala Val Val Thr Gly Asp 115 120 125Pro Asp Val Ala Arg Glu Leu Leu Gly Ser Ala Ala Phe Ala Asp Arg 130 135 140Pro Val Lys Glu Ser Ala Tyr Gly Leu Leu Phe His Arg Ala Ile Gly145 150 155 160Phe Ala Pro His Gly Ala Tyr Trp Arg Ala Leu Arg Arg Val Ala Ser 165 170 175Ala His Leu Phe Ser Pro Arg Gln Val Ala Ala Ser Ser Ala Gln Arg 180 185 190Ala Val Ile Ala Arg Gln Met Val Asp Ala Val Thr Thr Ala Ala Pro 195 200 205Ala Pro Ala Pro Ala Val Val Val Ala Arg Arg Phe Leu Lys Arg Ala 210 215 220Ser Leu His Asn Val Met Trp Ser Val Phe Gly Arg Arg Tyr Asp Leu225 230 235 240Leu Leu Leu Ala Ala Asp Gly Glu Glu Leu Lys Ala Leu Val Asp Glu 245 250 255Gly Tyr Asp Leu Leu Gly Gln Leu Asn Trp Ser Asp His Leu Pro Trp 260 265 270Leu Ala Arg Phe Asp Leu Gln Arg Thr Arg Ala Arg Cys Ser Ala Leu 275 280 285Val Pro Arg Val Asn Arg Phe Val Gly Asn Ile Ile Asp Glu His Arg 290 295 300Ala Arg Leu Gly Leu Gly Asp Thr Gly Gly Val Thr Asp Phe Thr Asp305 310 315 320Val Leu Leu Ser Leu Gln Gly Val Asp Lys Leu Ser Asp Ala Asp Met 325 330 335Val Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala 340 345 350Val Leu Met Glu Trp Val Leu Ala Arg Leu Val Leu His Gln Asp Val 355 360 365Gln Ser Lys Val Gln Glu Glu Leu Asp Arg Val Val Gly Pro Pro Gly 370 375 380Gln Ala Ala Ser Val Thr Glu Ser Asp Thr Ala Ser Leu Val Tyr Leu385 390 395 400Gln Ala Val Ile Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu 405 410 415Leu Ser Trp Ala Arg Leu Ala Thr Ser Asp Ala Arg Val Gly Gly Tyr 420 425 430His Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Thr 435 440 445His Asp Pro Ser Val Trp Ala Glu Pro Thr Glu Phe Arg Pro Glu Arg 450 455 460Phe Val Gly Ala Ser Ala Gly Ala Gly Ala Gly Ala Gly Ala Glu Asp465 470 475 480Val Pro Met Ile Met Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser 485 490 495Gly Arg Arg Ser Cys Pro Gly Lys Ser Leu Ala Leu Ala Thr Val Gly 500 505 510Phe Trp Val Ala Thr Leu Leu His Glu Phe Lys Trp Leu Pro Pro Cys 515 520 525Arg Gly Val Asp Leu Ser Glu Val Leu Arg Leu Ser Cys Glu Met Ala 530 535 540Ala Pro Leu Glu Ala Arg Val Val Pro Arg His Ala Val545 550 55565572PRTBrachypodium distachyon 65Met Ala Pro Ala Thr Ser Ala Ser Glu Asp Cys Ala Gly Trp Leu Leu1 5 10 15Tyr Ala Ser Leu Ala Ala Arg Cys Asn Asp Gly Gly Glu Ala Tyr Arg 20 25 30Ala Ala Val Phe Ala Met Ala Leu Leu Ala Thr Ser Phe Ile Leu Thr 35 40 45Ser Leu Leu His Trp Ala Ser Thr Pro Gly Gly Pro Ala Trp Gly Arg 50 55 60Tyr Arg Trp Thr Ser Thr Thr Ser Arg Ala Ala Ile Ser Thr Ser Pro65 70 75 80Arg Ile Pro Gly Pro Arg Gly Leu Pro Val Val Gly Ser Met Gly Leu 85 90 95Met Thr Gly Leu Ala His Arg Lys Leu Ala Ala Ala Val Ala Ala Gly 100 105 110Gly Asp Asp Glu Glu Glu Arg Ser Gln Arg Arg Arg Leu Met Ala Phe 115 120 125Ser Met Gly Glu Thr Arg Ala Val Val Ser Ser Asp Pro Ala Val Ala 130 135 140Arg Glu Leu Leu Ser Ser Pro Ala Phe Ala Asp Arg Pro Val Lys Glu145 150 155 160Ser Ala Tyr Gly Leu Leu Phe His Arg Ala Ile Gly Phe Ala Pro His 165 170 175Gly Ala Tyr Trp Arg Ser Leu Arg Arg Val Ala Ser Ala His Leu Phe 180 185 190Ser Pro Arg Gln Val Ala Ala Ser Ala Ala His Arg Ala Ala Ile Ala 195 200 205Arg Ser Met Val Gly Ser Val Ser Ala Ile Ala Met Gly Ser Gly Glu 210 215 220Val Glu Val Arg Arg Phe Leu Lys Arg Ala Ala Leu His Gly Val Met225 230 235 240Trp Ser Val Phe Gly Arg Arg Tyr Asp Gly Thr Ala Ala Pro Glu Leu 245 250 255Gly Lys Lys Glu Glu Glu Glu Leu Arg Ser Met Val Glu Glu Gly Tyr 260 265 270Glu Leu Leu Gly Lys Leu Asn Trp Ala Asp His Leu Pro Trp Leu Ala 275 280 285Arg Phe Asp Leu Gln Gly Ile Arg Ala Arg Cys Ala Ala Leu Val Pro 290 295 300Arg Val Asn Arg Phe Val Gly Lys Ile Val Asp Asp His Arg Ala Ala305 310 315 320Ala Ala Ala Asp Ala Gly Asp Arg Val Val Asp Phe Thr Asp Val Leu 325 330 335Leu Ser Leu Gln Gly Ala Asp Lys Leu Ser Asp Ala Asp Met Ile Ala 340 345 350Val Leu Trp Glu Met Val Phe Arg Gly Thr Asp Thr Met Ala Val Val 355 360 365Met Glu Trp Val Leu Ala Arg Leu Val Met His Gln Asp Val Gln Ala 370 375 380Arg Val Gln Glu Glu Leu Asp Arg Val Val Gly Pro

Gly Gln Ala Val385 390 395 400Ser Glu Ser Asp Ala Ala Arg Leu Val Tyr Leu Gln Ala Val Ile Lys 405 410 415Glu Thr Met Arg Leu His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg 420 425 430Leu Ala Thr Ser Asp Val His Val Gly Gly Phe Leu Val Pro Ala Gly 435 440 445Thr Thr Ala Met Val Asn Met Trp Ala Ile Thr His Asp Pro Thr Val 450 455 460Trp Ala Asp Pro Leu Glu Phe Asn Pro Asp Arg Phe Ile Val Gly Ala465 470 475 480Val Pro Leu Ser Glu Gly His His Asn Ala Val Pro Gly Ala Glu Phe 485 490 495Ser Ile Met Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg 500 505 510Arg Ile Cys Pro Gly Lys Pro Leu Ala Met Ala Ser Ile Gly Phe Trp 515 520 525Val Ala Thr Leu Leu His Glu Phe Lys Trp Thr Ser Ala Pro Arg Gly 530 535 540Asp Val Asp Leu Ser Glu Val Leu Arg Leu Ser Cys Glu Met Ala Ala545 550 555 560Pro Leu Lys Ala Arg Leu Thr Pro Arg Arg Pro Val 565 57066517PRTGlycine max 66Met Ser Ser Ser Glu Leu Ser Ser Phe Phe Leu Leu Pro Leu Ser Ala1 5 10 15Ile Leu Ser Phe Asp Ala Leu Leu Gly Val Met Phe Leu Val Ala Val 20 25 30Phe Gly Tyr Trp Leu Val Pro Gly Gly Leu Ala Trp Ala Leu Ser Lys 35 40 45Phe Lys Pro Ala Ile Pro Gly Pro Cys Gly Tyr Pro Val Val Gly Leu 50 55 60Val Trp Ala Phe Ile Gly Pro Leu Thr His Arg Val Leu Ala Lys Leu65 70 75 80Ala Glu Thr Phe Asp Ala Lys Pro Leu Met Ala Phe Ser Val Gly Phe 85 90 95Thr Arg Phe Ile Ile Ser Ser His Pro Asp Thr Ala Lys Glu Ile Leu 100 105 110Asn Ser Ser Ala Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Glu 115 120 125Leu Leu Phe His Arg Ala Met Gly Phe Ala Pro Tyr Gly Glu Tyr Trp 130 135 140Arg Asn Leu Arg Arg Ile Ser Ala Thr His Met Phe Ser Pro Lys Arg145 150 155 160Ile Ala Ala Gln Gly Val Phe Arg Ala Arg Val Gly Ala Gln Met Val 165 170 175Arg Glu Ile Val Gly Leu Met Gly Lys Asn Asp Val Val Glu Val Arg 180 185 190Lys Val Leu His Phe Gly Ser Leu Asn Asn Val Met Lys Ser Val Phe 195 200 205Gly Arg Ser Tyr Val Phe Gly Glu Gly Gly Asp Gly Cys Glu Leu Glu 210 215 220Glu Leu Val Ser Glu Gly Tyr Asp Leu Leu Gly Leu Phe Asn Trp Ser225 230 235 240Asp His Phe Pro Leu Leu Gly Trp Leu Asp Phe Gln Gly Val Arg Lys 245 250 255Arg Cys Arg Ser Leu Val Asp Arg Val Asn Val Phe Val Gly Lys Ile 260 265 270Ile Met Glu His Arg Val Lys Arg Asp Ala Glu Ser Gly Asp Phe Val 275 280 285Asp Val Leu Leu Asp Leu Glu Lys Glu Asp Arg Leu Asn His Ser Asp 290 295 300Met Val Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val305 310 315 320Ala Ile Leu Leu Glu Trp Ile Leu Ala Arg Met Val Leu His Pro Glu 325 330 335Ile Gln Ala Lys Ala Gln Cys Glu Ile Asp Ser Val Val Gly Ser Gly 340 345 350Cys Ser Val Thr Asp Asp Asp Leu Pro Asn Leu Pro Tyr Val Arg Ala 355 360 365Ile Val Lys Glu Thr Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser 370 375 380Trp Ala Arg Leu Ser Ile His Glu Thr Gln Ile Gly Asn His Phe Val385 390 395 400Pro Ala Gly Thr Thr Ala Met Val Asn Leu Trp Ala Ile Thr His Asp 405 410 415Gln Gln Val Trp Ser Glu Pro Glu Gln Phe Lys Pro Glu Arg Phe Leu 420 425 430Lys Asp Glu Asp Val Pro Ile Met Gly Ser Asp Leu Arg Leu Ala Pro 435 440 445Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Lys Ala Met Gly Leu Ala 450 455 460Thr Val Glu Leu Trp Leu Ala Val Phe Leu Gln Lys Phe Lys Trp Met465 470 475 480Pro Cys Asp Asp Ser Gly Val Asp Leu Ser Glu Cys Leu Lys Leu Ser 485 490 495Met Glu Met Lys His Ser Leu Ile Thr Lys Ala Val Ala Arg Pro Thr 500 505 510Ser Ser Leu Ala Met 51567525PRTOryza sativa 67Met Ala Leu Ser Ser Met Ala Ala Ala Gln Glu Ser Ser Leu Leu Leu1 5 10 15Phe Leu Leu Pro Thr Ser Ala Ala Ser Val Phe Pro Pro Leu Ile Ser 20 25 30Val Val Val Leu Ala Ala Leu Leu Leu Trp Leu Ser Pro Gly Gly Pro 35 40 45Ala Trp Ala Leu Ser Arg Cys Arg Gly Thr Pro Pro Pro Pro Gly Val 50 55 60Ala Gly Gly Ala Ala Ser Ala Leu Ser Gly Pro Ala Ala His Arg Val65 70 75 80Leu Ala Gly Ile Ser Arg Ala Val Glu Gly Gly Ala Ala Val Met Ser 85 90 95Leu Ser Val Gly Leu Thr Arg Leu Val Val Ala Ser Arg Pro Glu Thr 100 105 110Ala Arg Glu Ile Leu Val Ser Pro Ala Phe Gly Asp Arg Pro Val Lys 115 120 125Asp Ala Ala Arg Gln Leu Leu Phe His Arg Ala Met Gly Phe Ala Pro 130 135 140Ser Gly Asp Ala His Trp Arg Gly Leu Arg Arg Ala Ser Ala Ala His145 150 155 160Leu Phe Gly Pro Arg Arg Val Ala Gly Ser Ala Pro Glu Arg Glu Ala 165 170 175Ile Gly Ala Arg Ile Val Gly Asp Val Ala Ser Leu Met Ser Arg Arg 180 185 190Gly Glu Val Pro Leu Arg Arg Val Leu His Ala Ala Ser Leu Gly His 195 200 205Val Met Ala Thr Val Phe Gly Lys Arg His Gly Asp Ile Ser Ile Gln 210 215 220Asp Gly Glu Leu Leu Glu Glu Met Val Thr Glu Gly Tyr Asp Leu Leu225 230 235 240Gly Lys Phe Asn Trp Ala Asp His Leu Pro Leu Leu Arg Trp Leu Asp 245 250 255Leu Gln Gly Ile Arg Arg Arg Cys Asn Arg Leu Val Gln Lys Val Glu 260 265 270Val Phe Val Gly Lys Ile Ile Gln Glu His Lys Ala Lys Arg Ala Ala 275 280 285Gly Gly Val Ala Val Ala Asp Gly Val Leu Gly Asp Phe Val Asp Val 290 295 300Leu Leu Asp Leu Gln Gly Glu Glu Lys Met Ser Asp Ser Asp Met Ile305 310 315 320Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val Ala Ile 325 330 335Leu Met Glu Trp Val Met Ala Arg Met Val Met His Pro Glu Ile Gln 340 345 350Ala Lys Ala Gln Ala Glu Val Asp Ala Ala Val Gly Gly Arg Arg Gly 355 360 365Arg Val Ala Asp Gly Asp Val Ala Ser Leu Pro Tyr Ile Gln Ser Ile 370 375 380Val Lys Glu Thr Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser Trp385 390 395 400Ala Arg Leu Ala Val His Asp Ala Arg Val Gly Gly His Ala Val Pro 405 410 415Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ala His Asp Ala 420 425 430Ala Val Trp Pro Glu Pro Asp Ala Phe Arg Pro Glu Arg Phe Ser Glu 435 440 445Gly Glu Asp Val Gly Val Leu Gly Gly Asp Leu Arg Leu Ala Pro Phe 450 455 460Gly Ala Gly Arg Arg Val Cys Pro Gly Arg Met Leu Ala Leu Ala Thr465 470 475 480Ala His Leu Trp Leu Ala Gln Leu Leu His Ala Phe Asp Trp Ser Pro 485 490 495Thr Ala Ala Gly Val Asp Leu Ser Glu Arg Leu Gly Met Ser Leu Glu 500 505 510Met Ala Ala Pro Leu Val Cys Lys Ala Val Ala Arg Ala 515 520 52568520PRTGlycine max 68Met Ser Pro Asp Phe Thr Leu Leu Phe Phe Pro Glu Leu Ile Gln Pro1 5 10 15Pro Ile Val Thr Leu Gln Ala Ala Leu Cys Ile Leu Leu Leu Thr Phe 20 25 30Leu Leu Thr Phe Phe Leu Thr Pro Gly Gly Leu Ala Trp Ala Trp Ala 35 40 45Thr Lys Ser Ser Thr Arg Pro Ile Ile Pro Gly Pro Val Met Ala Leu 50 55 60Leu Ser Val Phe Thr Gly Ser Thr Pro His Arg Arg Leu Ser Met Leu65 70 75 80Ala Arg Ser Tyr His Ala Glu Lys Leu Met Ala Phe Ser Ile Gly Leu 85 90 95Thr Arg Phe Val Ile Ser Ser Glu Pro Glu Thr Ala Lys Glu Ile Leu 100 105 110Gly Ser Pro Gly Phe Ala Asp Arg Pro Val Lys Glu Ser Ala Tyr Gln 115 120 125Leu Leu Phe His Arg Ala Met Gly Phe Ala Pro Tyr Gly Glu Tyr Trp 130 135 140Arg Asn Leu Arg Arg Ile Ser Ala Leu His Leu Phe Ser Pro Lys Arg145 150 155 160Ile Thr Gly Ser Glu Ala Phe Arg Asn Glu Val Gly Leu Lys Met Val 165 170 175Asp Glu Val Lys Lys Val Met Lys Asp Asn Arg His Val Glu Val Lys 180 185 190Arg Ile Leu His Tyr Gly Ser Leu Asn Asn Val Met Met Thr Val Phe 195 200 205Gly Lys Cys Tyr Glu Phe Tyr Glu Gly Glu Gly Val Glu Leu Glu Ala 210 215 220Leu Val Ser Glu Gly Tyr Glu Leu Leu Gly Val Phe Asn Trp Ser Asp225 230 235 240His Phe Pro Val Leu Gly Trp Leu Asp Leu Gln Gly Val Arg Lys Arg 245 250 255Cys Arg Cys Leu Val Glu Lys Val Asn Ala Phe Val Gly Gly Val Ile 260 265 270Glu Glu His Arg Val Lys Arg Val Arg Gly Gly Cys Val Lys Asp Glu 275 280 285Gly Thr Gly Asp Phe Val Asp Val Leu Leu Asp Leu Glu Asn Glu Asn 290 295 300Lys Leu Ser Glu Ala Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe305 310 315 320Arg Gly Thr Asp Thr Val Ala Ile Leu Leu Glu Trp Ile Leu Ala Arg 325 330 335Met Val Leu His Pro Asp Ile Gln Ala Lys Ala Gln Arg Glu Ile Asp 340 345 350Ser Val Cys Gly Pro Tyr Arg Leu Val Ser Glu Ala Asp Met Pro Asn 355 360 365Leu Arg Tyr Leu Gln Gly Ile Val Lys Glu Thr Leu Arg Val His Pro 370 375 380Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Val His Asp Val Thr385 390 395 400Val Gly Gly Lys His Val Ile Pro Lys Gly Thr Thr Ala Met Val Asn 405 410 415Met Trp Ala Ile Thr His Asp Glu Arg Phe Trp Ala Glu Pro Glu Arg 420 425 430Phe Arg Pro Glu Arg Phe Val Glu Glu Glu Asp Val Asn Ile Met Gly 435 440 445Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro 450 455 460Gly Lys Ala Leu Gly Leu Ala Ser Val His Leu Trp Leu Ala Gln Leu465 470 475 480Leu Gln Asn Phe His Trp Val Gln Phe Asp Gly Val Ser Val Glu Leu 485 490 495Asp Glu Cys Leu Lys Leu Ser Met Glu Met Lys Lys Pro Leu Ala Cys 500 505 510Lys Ala Val Pro Arg Val Ala Val 515 52069445PRTSorghum bicolor 69Met Asp Ala Thr Thr Gln Asp Ser Leu Leu Phe Leu Phe Pro Ala Ala1 5 10 15Ala Thr Phe Leu Ser Pro Leu Leu Ala Val Leu Leu Val Ala Leu Ser 20 25 30Leu Leu Trp Leu Val Pro Gly Gly Pro Ala Trp Ala Leu Ile Ser Thr 35 40 45Ser Arg Ser Arg Ala Thr Pro Pro Pro Gly Ala Pro Gly Val Val Thr 50 55 60Ala Leu Ser Gly Pro Ala Ala His Arg Ala Leu Ala Ser Leu Ser Arg65 70 75 80Ser Leu Pro Gly Gly Ala Ala Leu Ser Ala Phe Ser Val Gly Leu Thr 85 90 95Arg Leu Val Val Ala Ser Gln Pro Asp Thr Ala Arg Glu Leu Leu Ala 100 105 110Ser Ala Ala Phe Ala Asp Arg Pro Val Lys Asp Ala Ala Arg Gly Leu 115 120 125Leu Phe His Arg Ala Met Gly Phe Ala Pro Ser Gly Asp Tyr Trp Arg 130 135 140Ala Leu Cys Arg Ile Ser Ser Ala Tyr Leu Phe Ser Pro Arg Ser Glu145 150 155 160Ser Ala Thr Ala Pro Arg Arg Val Thr Ile Gly Glu Arg Met Leu Arg 165 170 175Asp Leu Ser Asp Ala Ile Gly Arg Leu Arg Arg Ser Leu Val Ser Arg 180 185 190Val Asn Val Phe Val Ala Arg Ile Ile Glu Glu His Arg Gln Lys Lys 195 200 205Lys Asp Asp Val Ala Asn Asn Gly Glu Ser Ala Ala Gly Asp Phe Val 210 215 220Asp Val Leu Leu Gly Leu Glu Gly Glu Glu Lys Leu Ser Asp Ser Asp225 230 235 240Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Thr Val 245 250 255Ala Ile Leu Leu Glu Trp Val Met Ala Arg Met Val Leu His Pro Gly 260 265 270Ile Gln Ser Lys Ala Gln Ala Glu Leu Asp Ala Val Val Gly Arg Gly 275 280 285Gly Ala Val Ser Asp Ala Asp Val Ser Arg Leu Pro Tyr Leu Gln Arg 290 295 300Val Val Lys Glu Thr Leu Arg Val His Pro Pro Gly Pro Leu Leu Ser305 310 315 320Trp Ala Arg Leu Ala Val His Asp Ala Val Val Gly Gly His Leu Val 325 330 335Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ala Arg Asp 340 345 350Pro Ala Val Trp Ala Asp Pro Thr Ala Phe Arg Pro Glu Arg Phe Glu 355 360 365Glu Glu Asp Val Ser Val Leu Gly Gly Asp Leu Arg Leu Ala Pro Phe 370 375 380Gly Ala Gly Arg Arg Val Cys Pro Gly Lys Thr Leu Ala Leu Ala Thr385 390 395 400Val His Leu Trp Leu Ala Gln Leu Leu His Arg Phe Gln Trp Ala Pro 405 410 415Ala Asp Gly Gly Val Asp Leu Ala Glu Arg Leu Gly Met Ser Leu Glu 420 425 430Met Glu Lys Pro Leu Val Cys Lys Pro Thr Pro Arg Trp 435 440 44570548PRTOryza sativa 70Met Asp Ala Thr Leu Gly Ala Ser Thr Thr His Gly Tyr Leu Leu Leu1 5 10 15Leu Pro Ala Asn Ser Thr Thr Phe Phe Ser Pro Leu Leu Ala Ala Leu 20 25 30Leu Ala Val Thr Ser Leu Leu Trp Leu Val Pro Gly Gly Pro Ala Trp 35 40 45Ala Leu Ser Arg Cys Arg Arg Pro Pro Pro Gly Ala Pro Gly Ala Leu 50 55 60Ala Ala Leu Ala Gly Pro Ala Ala His Arg Ala Leu Ala Ala Met Ser65 70 75 80Arg Ser Val Pro Gly Gly Ala Ala Leu Ala Ser Phe Ser Val Gly Leu 85 90 95Thr Arg Phe Val Val Ala Ser Arg Pro Asp Thr Ala Arg Glu Leu Leu 100 105 110Ser Ser Ala Ala Phe Ala Asp Arg Pro Val Lys Asp Ala Ala Arg Gly 115 120 125Leu Leu Phe His Arg Ala Met Gly Phe Ala Pro Ser Gly Asp Tyr Trp 130 135 140Arg Ala Leu Arg Arg Val Ser Ala Asn His Leu Phe Thr Pro Arg Arg145 150 155 160Val Ala Ala Ser Ala Pro Arg Arg Leu Ala Ile Gly Glu Arg Met Leu 165 170 175Asp Arg Leu Ser Ala Leu Ala Gly Gly Glu Ile Gly Met Arg Arg Val 180 185 190Leu His Ala Ala Ser Leu Asp His Val Met Asp Thr Val Phe Gly Thr 195 200 205Arg Tyr Asp Gly Asp Ser Gln Glu Gly Ala Glu Leu Glu Ala Met Val 210 215 220Lys Glu Gly Tyr Asp Leu Leu Gly Met Phe Asn Trp Gly Asp His Leu225 230 235 240Pro Leu Leu Lys Trp Leu Asp Leu Gln Gly Val Arg Arg Arg Cys Arg 245 250 255Thr Leu Val Gln Arg Val Asp Val Phe Val Arg Ser Ile Ile Asp Glu 260

265 270His Arg Gln Arg Lys Arg Arg Thr Gly Gly Asn Gly Gly Gly Glu Glu 275 280 285Leu Pro Gly Asp Phe Val Asp Val Leu Leu Gly Leu Gln Gly Glu Glu 290 295 300Lys Met Thr Glu Ser Asp Met Val Ala Val Leu Trp Val Thr Lys Asp305 310 315 320Pro Ser Asp Met His Ala Ser Ile Arg Ser Ile Leu Cys Ile Ala Ile 325 330 335Asn Gly Phe Met Asp Ile Phe Asp Leu Ala Arg Val Gln Glu Met Ile 340 345 350Phe Arg Gly Thr Asp Thr Val Ala Ile Leu Leu Glu Trp Ile Met Ala 355 360 365Arg Met Val Leu His Pro Asp Ile Gln Ala Lys Ala Gln Ala Glu Leu 370 375 380Asp Ala Val Val Gly Arg Gly Arg Ala Val Ser Asp Gly Asp Val Ala385 390 395 400Gly Leu Arg Tyr Leu Gln Cys Val Val Lys Glu Ala Leu Arg Val His 405 410 415Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Val Arg Asp Ala 420 425 430His Val Gly Gly His Val Val Pro Ala Gly Thr Thr Ala Met Val Asn 435 440 445Met Trp Ala Ile Ala His Asp Pro Glu Leu Trp Pro Glu Pro Asp Glu 450 455 460Phe Arg Pro Glu Arg Phe Ala Glu Glu Asp Val Ser Val Leu Gly Gly465 470 475 480Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Ala Cys Pro Gly 485 490 495Lys Thr Leu Ala Leu Ala Thr Val His Leu Trp Leu Ala Gln Leu Leu 500 505 510His Arg Phe Glu Trp Ala Pro Val Gly Gly Gly Val His Leu Leu Glu 515 520 525Arg Leu Asn Met Ser Leu Glu Met Glu Lys Pro Leu Val Cys Lys Ala 530 535 540Lys Pro Arg Trp54571522PRTGlycine max 71Met Ile Pro Thr Leu Val Cys Ile Gly Thr Thr Ile Phe Gln Ser Thr1 5 10 15Leu Ser Ser Tyr Ser Leu Ser Phe Ile Ser Leu Phe Leu Ser Thr Ser 20 25 30Leu Ala Leu Leu Ala Ile Ser Leu Asn Tyr Trp Leu Val Pro Gly Gly 35 40 45Phe Ala Trp Arg Lys Tyr His Ser Arg Tyr Lys Gly His Ala Lys Val 50 55 60Ser Gly Pro Met Gly Trp Pro Ile Leu Gly Thr Leu Pro Ala Met Gly65 70 75 80Pro Leu Ala His Arg Lys Leu Ala Ala Met Ala Thr Ser Pro Lys Ala 85 90 95Lys Lys Leu Met Thr Leu Ser Leu Gly Thr Asn Pro Val Val Ile Ser 100 105 110Ser His Pro Glu Thr Ala Arg Glu Ile Leu Cys Gly Ser Asn Phe Ala 115 120 125Asp Arg Pro Val Lys Glu Ser Ala Arg Met Leu Met Phe Glu Arg Ala 130 135 140Ile Gly Phe Ala Pro Tyr Gly Thr Tyr Trp Arg His Leu Arg Lys Val145 150 155 160Ala Ile Thr His Met Phe Ser Pro Arg Arg Ile Ser Asp Leu Glu Ser 165 170 175Leu Arg Gln His Val Val Gly Glu Met Val Met Arg Ile Trp Lys Glu 180 185 190Met Gly Asp Lys Gly Val Val Glu Val Arg Gly Ile Leu Tyr Glu Gly 195 200 205Ser Leu Ser His Met Leu Glu Cys Val Phe Gly Ile Asn Asn Ser Leu 210 215 220Gly Ser Gln Thr Lys Glu Ala Leu Gly Asp Met Val Glu Glu Gly Tyr225 230 235 240Asp Leu Ile Ala Lys Phe Asn Trp Ala Asp Tyr Phe Pro Phe Gly Phe 245 250 255Leu Asp Phe His Gly Val Lys Arg Arg Cys His Lys Leu Ala Thr Lys 260 265 270Val Asn Ser Val Val Gly Lys Ile Val Glu Glu Arg Lys Asn Ser Gly 275 280 285Lys Tyr Val Gly Gln Asn Asp Phe Leu Ser Ala Leu Leu Leu Leu Pro 290 295 300Lys Glu Glu Ser Ile Gly Asp Ser Asp Val Val Ala Ile Leu Trp Glu305 310 315 320Met Ile Phe Arg Gly Thr Asp Thr Ile Ala Ile Leu Leu Glu Trp Ile 325 330 335Met Ala Met Met Val Leu His Gln Asp Val Gln Met Lys Ala Arg Gln 340 345 350Glu Ile Asp Ser Cys Ile Lys Gln Asn Gly Tyr Met Arg Asp Ser Asp 355 360 365Ile Pro Asn Leu Pro Tyr Leu Gln Ala Ile Val Lys Glu Val Leu Arg 370 375 380Leu His Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Ile His385 390 395 400Asp Val His Val Asp Lys Val Ile Val Pro Ala Gly Thr Thr Ala Met 405 410 415Val Asn Met Trp Ala Ile Ser His Asp Ser Ser Ile Trp Glu Asp Pro 420 425 430Trp Ala Phe Lys Pro Glu Arg Phe Met Lys Glu Asp Val Ser Ile Met 435 440 445Gly Ser Asp Met Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Val Cys 450 455 460Pro Gly Lys Thr Leu Gly Leu Ala Thr Val His Leu Trp Leu Ala Gln465 470 475 480Leu Leu His His Phe Ile Trp Ile Pro Val Gln Pro Val Asp Leu Ser 485 490 495Glu Cys Leu Lys Leu Ser Leu Glu Met Lys Lys Pro Leu Arg Cys Gln 500 505 510Val Ile Arg Arg Phe Asn Thr Ile Ser Ser 515 52072534PRTZea mays 72Met Thr Leu Ile Pro Ala Ile Ser Gly Glu Gln His Gly Asn Met Ala1 5 10 15Thr Val Ala Thr Ser Phe Ala Tyr Leu Ala Ile Phe Ala Cys Leu Ala 20 25 30Trp Val Gly Ala Ser Leu Leu Tyr Trp Ala His Pro Gly Gly Pro Ala 35 40 45Trp Gly Lys Tyr Trp Arg Ala Arg Gly Lys Lys Pro Ser Ala Ala Ile 50 55 60Pro Gly Pro Lys Gly Leu Pro Val Val Gly Ser Leu Gly Leu Met Ser65 70 75 80Gly Leu Ala His Arg Ser Leu Ala Asp Glu Ala Ser Arg Arg Pro Gly 85 90 95Ala Lys Arg Leu Met Ala Leu Ser Leu Gly Pro Val Arg Ala Val Val 100 105 110Thr Ser His Pro Asp Val Ala Lys Glu Ile Leu Asp Ser Pro Ala Phe 115 120 125Ala Ala Arg Pro Leu Asn His Ala Ala Tyr Gly Leu Met Phe His Arg 130 135 140Ser Ile Gly Phe Ala Glu His Gly Pro Tyr Trp Arg Ala Leu Arg Arg145 150 155 160Val Ala Ala Gly His Leu Phe Gly Pro Arg Gln Val Glu Ala Phe Ala 165 170 175Pro Tyr Arg Ala Ala Val Ala Glu Gly Ile Val Ala Ala Leu Leu Arg 180 185 190Ala Gly Ser Gly Gly Ala Val Val Gln Val Arg Gly Leu Leu Arg Arg 195 200 205Ala Ser Leu Tyr Tyr Ile Met Arg Phe Val Phe Gly Lys Glu Tyr Asp 210 215 220Val Ser Arg Val Val Pro Pro Ser Gly Gly Glu Glu Val Glu Glu Leu225 230 235 240Leu Glu Met Val His Glu Gly Tyr Glu Leu Leu Gly Met Glu Asn Leu 245 250 255Cys Asp Tyr Phe Pro Gly Leu Ala Ala Leu Asp Pro Gln Gly Val Gly 260 265 270Ala Arg Cys Ala Glu Leu Met Pro Arg Val Asn Arg Phe Val His Gly 275 280 285Val Ile Gln Glu His Arg Ala Lys Ala Val Ala Gly Gly Asp Ala Arg 290 295 300Asp Phe Val Asp Ile Leu Leu Ser Leu Gln Glu Ser Glu Gly Leu Ala305 310 315 320Asp Ala Asp Ile Ala Ser Val Leu Trp Glu Met Ile Phe Arg Gly Thr 325 330 335Asp Ala Met Ala Val Leu Met Glu Trp Thr Leu Ala Arg Leu Val Leu 340 345 350His Arg Asp Val Gln Ala Lys Ala His Arg Glu Leu Asp Lys Val Val 355 360 365Gly Ala Asp Ser Gln Thr Thr Glu Ser Ala Ala Pro Tyr Leu Gln Ala 370 375 380Leu Leu Lys Glu Ala Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser385 390 395 400Trp Arg His Arg Ala Ile Ser Asp Thr Tyr Val Asp Gly His Leu Val 405 410 415Pro Ala Gly Thr Thr Ala Met Val Asn Gln Trp Ala Ile Ser Arg Asp 420 425 430Pro Glu Val Trp Asp Ala Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu 435 440 445Pro Gly Gly Glu Gly Gln Asp Val Ser Val Leu Gly Ala Asp Gly Arg 450 455 460Leu Val Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Lys Ser Leu465 470 475 480Ala Met Thr Thr Val Thr Ser Trp Met Ala Thr Leu Leu His Glu Phe 485 490 495Glu Trp Leu Pro Ala Ser Asp Asp Thr Gly Asp Val Asp Leu Ser Glu 500 505 510Val Leu Arg Leu Ser Cys Glu Met Ala Val Pro Leu Glu Val Arg Val 515 520 525Arg Pro Arg Ser Ser Val 53073514PRTGlycine max 73Met Lys Pro Thr Ala Thr Phe Phe Phe Leu Leu Ser Thr Thr Thr Leu1 5 10 15Leu Val Cys Leu Cys Leu Gly Thr Thr Thr Phe Gln Thr Thr Leu Phe 20 25 30Ile Thr Phe Phe Thr Ile Ser Leu Asn Tyr Trp Leu Val Pro Gly Gly 35 40 45Phe Ala Trp Arg Asn Tyr His Ser Tyr His Thr Asn Glu Lys Pro Asn 50 55 60Lys Lys Leu Thr Gly Pro Met Gly Trp Pro Ile Leu Gly Ser Leu Pro65 70 75 80Leu Met Gly Ser Leu Ala His Gln Lys Leu Ala Ala Leu Ala Ala Thr 85 90 95Leu Asn Ala Lys Arg Leu Met Ala Leu Ser Leu Gly Pro Thr Pro Val 100 105 110Val Ile Ser Ser His Pro Glu Thr Ala Arg Glu Ile Leu Leu Gly Ser 115 120 125Ser Phe Ser Asp Arg Pro Ile Lys Glu Ser Ala Arg Ala Leu Met Phe 130 135 140Glu Arg Ala Ile Gly Phe Ala Pro Ser Gly Thr Tyr Trp Arg His Leu145 150 155 160Arg Arg Ile Ala Ala Phe His Met Phe Ser Pro Arg Arg Ile Gln Gly 165 170 175Leu Glu Gly Leu Arg Gln Arg Val Gly Asp Asp Met Val Lys Ser Ala 180 185 190Trp Lys Glu Met Glu Met Lys Gly Val Val Glu Val Arg Gly Val Phe 195 200 205Gln Glu Gly Ser Leu Cys Asn Ile Leu Glu Ser Val Phe Gly Ser Asn 210 215 220Asp Lys Ser Glu Glu Leu Gly Asp Met Val Arg Glu Gly Tyr Glu Leu225 230 235 240Ile Ala Met Leu Asn Leu Glu Asp Tyr Phe Pro Leu Lys Phe Leu Asp 245 250 255Phe His Gly Val Lys Arg Arg Cys His Lys Leu Ala Ala Lys Val Gly 260 265 270Ser Val Val Gly Gln Ile Val Glu Asp Arg Lys Arg Glu Gly Ser Phe 275 280 285Val Val Lys Asn Asp Phe Leu Ser Thr Leu Leu Ser Leu Pro Lys Glu 290 295 300Glu Arg Leu Ala Asp Ser Asp Met Ala Ala Ile Leu Trp Glu Met Val305 310 315 320Phe Arg Gly Thr Asp Thr Val Ala Ile Leu Leu Glu Trp Val Met Ala 325 330 335Arg Met Val Leu His Gln Asp Val Gln Lys Lys Ala Arg Glu Glu Ile 340 345 350Asp Thr Cys Ile Gly Gln Asn Ser His Val Arg Asp Ser Asp Ile Ala 355 360 365Asn Leu Pro Tyr Leu Gln Ala Ile Val Lys Glu Val Leu Arg Leu His 370 375 380Pro Pro Gly Pro Leu Leu Ser Trp Ala Arg Leu Ala Val Asn Asp Val385 390 395 400His Val Asp Lys Val Leu Val Pro Ala Gly Thr Thr Ala Met Val Asn 405 410 415Met Trp Ala Ile Ser His Asp Ser Ser Ile Trp Glu Asp Pro Trp Ala 420 425 430Phe Lys Pro Glu Arg Phe Leu Lys Glu Asp Val Ser Ile Met Gly Ser 435 440 445Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly 450 455 460Arg Ala Leu Gly Leu Ala Thr Thr His Leu Trp Leu Ala Gln Leu Leu465 470 475 480Arg His Phe Ile Trp Leu Pro Ala Gln Pro Val Asp Leu Ser Glu Cys 485 490 495Leu Arg Leu Ser Met Glu Met Lys Thr Pro Leu Arg Cys Leu Val Val 500 505 510Arg Arg74235PRTSorghum bicolor 74Met Asp Phe Thr Asp Val Leu Leu Ser Leu Asn Gly Asp Asp Lys Leu1 5 10 15Ser Asp Ala Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg Gly 20 25 30Thr Asp Thr Val Ala Val Leu Ile Glu Trp Val Leu Ala Arg Leu Val 35 40 45Leu His Gln Asp Val Gln Arg Lys Val His Asp Glu Leu Asp Arg Val 50 55 60Val Gly Pro Gly Glu Ala Val Thr Glu Ser Asp Thr Ala Ser Leu Val65 70 75 80Tyr Leu Gln Ala Val Ile Lys Glu Val Leu Arg Leu His Pro Pro Gly 85 90 95Pro Leu Leu Ser Trp Ala Arg Leu Ala Thr Ser Asp Val Asn Val Gly 100 105 110Gly His Leu Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala 115 120 125Ile Thr His Asp Ala Ser Val Trp Pro Glu Pro Thr Glu Phe Arg Pro 130 135 140Glu Arg Phe Val Ala Ala Ala Gly Gly Glu Asp Val Val Pro Ile Met145 150 155 160Gly Ser Asp Leu Arg Leu Ala Pro Phe Gly Ser Gly Arg Arg Ser Cys 165 170 175Pro Gly Lys Ser Leu Ala Val Ala Thr Val Gly Phe Trp Val Ala Thr 180 185 190Leu Leu His Glu Phe Glu Trp Leu Pro Cys Gly Gly Gly Gly Gly Val 195 200 205Asp Leu Ser Glu Val Leu Arg Leu Ser Cys Glu Met Ala Ala Pro Leu 210 215 220Glu Ala Arg Val Val Pro Arg Arg His Ala Val225 230 23575508PRTGlycine max 75Met Lys Pro Thr Ala Thr Phe Phe Phe Leu Leu Pro Ser Thr Thr Leu1 5 10 15Val Val Cys Leu Cys Leu Gly Ile Gly Thr Thr Thr Leu Phe Ile Thr 20 25 30Leu Leu Ala Ile Ser Leu Asn Tyr Trp Leu Val Pro Gly Gly Phe Ala 35 40 45Trp Arg Asn Tyr Asp Tyr Tyr Gln Thr Lys Lys Lys Leu Thr Gly Pro 50 55 60Met Gly Trp Pro Ile Leu Gly Thr Leu Pro Leu Met Gly Ser Leu Ala65 70 75 80His Gln Lys Leu Ala Ala Leu Ala Thr Ser Leu Asn Ala Lys Arg Leu 85 90 95Met Ala Leu Ser Leu Gly Pro Thr Pro Val Val Ile Ser Ser His Pro 100 105 110Glu Thr Ala Arg Glu Ile Leu Leu Gly Ser Ser Phe Ser Asp Arg Pro 115 120 125Ile Lys Glu Ser Ala Arg Ala Leu Met Phe Glu Arg Ala Ile Gly Phe 130 135 140Ala His Ser Gly Thr Tyr Trp Arg His Leu Arg Arg Ile Ala Ala Phe145 150 155 160His Met Phe Ser Pro Arg Arg Ile His Gly Leu Glu Gly Leu Arg Gln 165 170 175Arg Val Gly Asp Asp Met Val Lys Ser Ala Trp Arg Glu Met Gly Glu 180 185 190Lys Gly Val Val Glu Val Arg Arg Val Phe Gln Glu Gly Ser Leu Cys 195 200 205Asn Ile Leu Glu Ser Val Phe Gly Ser Asn Asp Lys Ser Glu Glu Leu 210 215 220Arg Asp Met Val Arg Glu Gly Tyr Glu Leu Ile Ala Met Phe Asn Leu225 230 235 240Glu Asp Tyr Phe Pro Phe Lys Phe Leu Asp Phe His Gly Val Lys Arg 245 250 255Arg Cys His Lys Leu Ala Ala Lys Val Gly Ser Val Val Gly Gln Ile 260 265 270Val Glu Glu Arg Lys Arg Asp Gly Gly Phe Val Gly Lys Asn Asp Phe 275 280 285Leu Ser Thr Leu Leu Ser Leu Pro Lys Glu Glu Arg Leu Ala Asp Ser 290 295 300Asp Leu Val Ala Ile Leu Trp Glu Met Val Phe Arg Gly Thr Asp Thr305 310 315 320Val Ala Ile Leu Leu Glu Trp Val Met Ala Arg Met Val Leu His Gln 325 330 335Asp Leu Gln Lys Lys Ala Arg Glu Glu Ile Asp Thr Cys Val Gly Gln 340 345 350Asn Ser His Val Arg Asp Ser Asp Ile Ala Asn Leu Pro Tyr Leu Gln 355 360 365Ala Ile Val Lys Glu Val Leu Arg Leu His Pro Pro Gly Pro Leu Leu 370 375

380Ser Trp Ala Arg Leu Ala Val His Asp Val His Ala Asp Lys Val Leu385 390 395 400Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ser His 405 410 415Asp Ser Ser Ile Trp Glu Asp Pro Trp Ala Phe Lys Pro Glu Arg Phe 420 425 430Leu Lys Glu Asp Val Ser Ile Met Gly Ser Asp Leu Arg Leu Ala Pro 435 440 445Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Arg Ala Leu Gly Leu Ala 450 455 460Thr Ala His Leu Trp Leu Ala Gln Leu Leu Arg His Phe Ile Trp Leu465 470 475 480Pro Ala Gln Thr Val Asp Leu Ser Glu Cys Leu Arg Leu Ser Met Glu 485 490 495Met Lys Thr Pro Leu Arg Cys Leu Val Val Arg Arg 500 50576517PRTSorghum bicolor 76Met Val Leu Thr Met Ala Thr Gly Gln Glu Asp Ser Leu Leu Leu Leu1 5 10 15Leu Leu Pro Thr Thr Ser Pro Leu Pro Pro Leu Met Ala Val Phe Ile 20 25 30Leu Ala Ala Val Leu Leu Trp Leu Ser Pro Gly Gly Pro Ala Trp Ala 35 40 45Leu Ser Arg Cys Arg Arg Pro Pro Ser Gly Pro Thr Gly Val Val Thr 50 55 60Ala Leu Ser Ser Pro Val Ala His Arg Thr Leu Ala Ala Leu Ser His65 70 75 80Ala Val Asp Gly Gly Lys Ala Leu Met Ala Phe Ser Val Gly Leu Thr 85 90 95Arg Leu Val Val Ser Ser Gln Pro Asp Thr Ala Arg Glu Ile Leu Val 100 105 110Asn Pro Ala Phe Ser Asp Arg Pro Ile Lys Asp Ala Ala Arg His Leu 115 120 125Leu Phe His Arg Ala Met Gly Phe Ala Pro Ser Gly Asp Ala His Trp 130 135 140Arg Gly Leu Arg Arg Leu Ala Ala Asn His Leu Phe Gly Pro Arg Arg145 150 155 160Val Ala Ala Ala Ala His His Arg Val Ser Ile Gly Glu Ala Met Val 165 170 175Ala Asp Val Ala Ala Ala Met Ala Arg His Gly Glu Val Ser Leu Lys 180 185 190Arg Val Leu His Ile Ala Ser Leu Asn His Ile Met Ala Thr Val Phe 195 200 205Gly Lys His Tyr Asp Met Asp Ser Gln Glu Gly Val Leu Leu Glu Glu 210 215 220Met Val Thr Glu Gly Tyr Asp Leu Leu Gly Thr Phe Asn Trp Ala Asp225 230 235 240His Leu Pro Leu Ile Lys His Leu Asp Leu Gln Gly Val Arg Arg Arg 245 250 255Cys Asn Arg Leu Val Gln Lys Val Glu Val Phe Val Gly Lys Ile Ile 260 265 270Gln Glu His Arg Ala Arg Arg Ala Asn Gly Gly Val Asp Asp Glu Tyr 275 280 285Met Gly Asp Phe Val Asp Val Leu Leu Asp Leu Glu Gly Glu Glu Lys 290 295 300Leu Ser Glu Ser Asp Met Ile Ala Val Leu Trp Glu Met Ile Phe Arg305 310 315 320Gly Ala Asp Thr Val Ala Ile Leu Met Glu Trp Ile Met Ala Arg Met 325 330 335Ala Leu His Pro Glu Ile Gln Ser Lys Ala Gln Ala Glu Leu Asp Gly 340 345 350Val Val Val Gly Gly Val Ala Asp Ala Asp Val Gly Asn Leu Pro Tyr 355 360 365Ile Gln Cys Ile Val Lys Glu Thr Leu Arg Met His Pro Pro Gly Pro 370 375 380Leu Leu Ser Trp Ala Arg Leu Ala Ile His Asp Ala His Val Gly Gly385 390 395 400His Leu Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ser Ile 405 410 415Ala His Asp Pro Ala Ile Trp Ala Glu Pro Glu Lys Phe Arg Pro Glu 420 425 430Arg Phe Gln Glu Glu Asp Val Ser Val Leu Gly Ser Asp Leu Arg Leu 435 440 445Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro Gly Lys Met Leu Ala 450 455 460Leu Ala Thr Thr His Leu Trp Ile Ala Gln Leu Leu His Glu Phe Glu465 470 475 480Trp Ala Pro Ala Ala Ala Asn Gly Gly Val Asp Leu Ser Glu Arg Leu 485 490 495Asn Met Ser Leu Glu Met Ala Thr Pro Leu Val Cys Lys Ala Val Pro 500 505 510Arg Ala Gln Leu Ala 51577549PRTSorghum bicolor 77Met Glu Ser Ser Val Glu Ser Trp Trp Val Leu Pro Met Thr Leu Ile1 5 10 15Pro Ala Ile Ser Gly Gln Gln His Glu Asn Met Ala Thr Ile Ala Thr 20 25 30Ser Phe Val Tyr Leu Ala Ile Phe Ala Cys Leu Ala Trp Ala Gly Ala 35 40 45Ser Leu Leu Tyr Trp Ala His Pro Gly Gly Pro Ala Trp Gly Lys Tyr 50 55 60Trp Arg Ala Lys Gly Lys Pro Ser Ser Thr Ile Pro Gly Pro Lys Gly65 70 75 80Leu Pro Val Val Gly Ser Leu Gly Leu Met Ser Gly Leu Ala His Cys 85 90 95Ser Leu Ala Asp Glu Ala Ser Arg Arg Pro Gly Ala Lys Arg Leu Met 100 105 110Ala Leu Ser Leu Gly Pro Val Arg Ala Val Val Thr Ser His Pro Asp 115 120 125Val Ala Lys Glu Ile Leu Asp Asn Pro Ala Phe Ala Asp Arg Pro Leu 130 135 140Asn His Ala Ala Tyr Gly Leu Met Phe His Arg Ser Ile Gly Phe Ala145 150 155 160Glu His Gly Pro Tyr Trp Arg Ala Leu Arg Arg Val Ala Ala Gly His 165 170 175Leu Phe Gly Pro Arg Gln Val Glu Ala Phe Ala Pro Tyr Arg Ala Ala 180 185 190Val Gly Glu Gly Ile Val Ala Ala Leu His Gly Ala Gly Gly Gly Val 195 200 205Val Gln Val Arg Gly Leu Leu Arg Arg Ala Ser Leu Tyr Tyr Ile Met 210 215 220Arg Phe Val Phe Gly Lys Glu Tyr Asp Val Ser Arg Ala Val Pro Ala225 230 235 240Ser Gly Lys Glu Glu Val Glu Glu Leu Leu Glu Met Val His Glu Gly 245 250 255Tyr Glu Leu Leu Gly Met Glu Asn Trp Cys Asp Tyr Phe Pro Gly Leu 260 265 270Ala Ala Leu Asp Pro Gln Gly Val Gly Ala Arg Cys Ala Glu Leu Met 275 280 285Pro Arg Val Asn Arg Phe Val His Gly Ile Ile Gln Glu Arg Arg Ala 290 295 300Lys Ala Ile Ala Gly Gly Asp Ala Arg Asp Phe Val Asp Ile Leu Leu305 310 315 320Ser Leu Gln Glu Ser Glu Arg Leu Ala Asp Ala Asp Ile Ala Ala Val 325 330 335Leu Trp Glu Met Ile Phe Arg Gly Thr Asp Ala Met Ala Val Leu Met 340 345 350Glu Trp Thr Leu Ala Arg Leu Val Leu His Arg Asp Val Gln Ala Lys 355 360 365Ala His Arg Glu Leu Asp Glu Val Val Gly Gly Asn Ser Gln Val Val 370 375 380Thr Glu Ser Ala Ala Ala Pro Ser Leu Pro Tyr Leu Gln Ala Leu Leu385 390 395 400Lys Glu Ala Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser Trp Arg 405 410 415His Arg Ala Ile Ser Asp Thr Tyr Val Asp Gly His Leu Val Pro Ala 420 425 430Gly Thr Thr Ala Met Val Asn Gln Trp Ala Ile Ser Arg Asp Pro Glu 435 440 445Val Trp Asp Ala Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Pro Gly 450 455 460Gly Glu Gly Gln Asp Val Ser Val Leu Gly Ala Asp Gly Arg Leu Val465 470 475 480Pro Phe Gly Ser Gly Arg Arg Ser Cys Pro Gly Lys Ser Leu Ala Met 485 490 495Thr Thr Val Thr Thr Trp Met Ala Thr Leu Leu Asn Glu Phe Glu Trp 500 505 510Leu Pro Ala Ser Asp Asp Thr Gly Gly Asp Val Asp Leu Ser Glu Val 515 520 525Leu Arg Leu Ser Cys Glu Met Ala Val Pro Leu Glu Val Arg Val Arg 530 535 540Pro Arg Ser Gly Met54578539PRTSorghum bicolor 78Met Lys Thr Glu Val Ile Thr Thr Met Ile Ser Leu Val Phe Leu Val1 5 10 15His Phe Ala Ile Thr Ile Ser Pro Asn Ala Gln Pro Ser Trp Leu Phe 20 25 30Ser Leu Met Ser Leu Ser Leu Ala Val Val Ala Val Ile Val Pro Leu 35 40 45Val Val Thr Thr Thr Cys His Ala Arg Lys Asn Thr Asp Ala Thr Thr 50 55 60Thr Ile Pro Gly Pro Arg Gly Trp Pro Leu Val Gly Ser Leu Leu Val65 70 75 80Val Ser Gly Pro Leu Met His Arg Arg Leu Ala Ala Leu Ala Asp Ala 85 90 95His Ser Ala Arg Arg Leu Met Ser Leu Thr Leu Gly Ala Thr Pro Val 100 105 110Val Ile Ser Ser His Pro Glu Thr Ala Arg Asp Ile Leu Ser Gly Ala 115 120 125Ala Phe Val Asp Arg Pro Pro Lys Ala Ala Ala Arg Glu Leu Met Phe 130 135 140Cys Arg Ala Ile Gly Phe Ala Pro Thr Gly Glu Tyr Trp Arg Arg Leu145 150 155 160Arg Arg Ile Thr Gly Ala Gly Met Leu Ser Pro Arg Arg Met Ala Met 165 170 175Leu Arg Gly Leu Arg Cys Arg Val Ala Asp Ser Met Ile Gln Arg Val 180 185 190Ala Asp Gln Met Glu Arg Ser Gly Glu Val Ala Met Arg Ala Leu Leu 195 200 205Gln Arg Ala Ser Leu Glu Ser Met Val Gly Ser Val Leu Gly Leu Glu 210 215 220Gly Asp Ala Val Cys Glu Glu Leu Gly Glu Met Val Arg Glu Gly Tyr225 230 235 240Glu Leu Val Gly Met Phe Asn Leu Glu Asp His Tyr Tyr Lys Thr Ser 245 250 255Trp Gly Pro Leu Met Asp Leu Trp Gly Val Arg Pro Met Cys Arg Glu 260 265 270Leu Ala Ala Met Val Arg Gly Tyr Phe Gly Lys Ile Ile Gln Glu Arg 275 280 285Arg Leu Ala Gly Asp Cys His Glu Arg Ala Asp Leu Leu Ser Tyr Met 290 295 300Leu Ser Leu Pro Glu Glu Glu Lys Leu Glu Asp Ser Asp Val Ile Ala305 310 315 320Val Leu Trp Glu Met Ile Phe Arg Gly Val Asp Val Val Ala Ile Leu 325 330 335Leu Glu Trp Ala Met Ala Arg Met Ser Leu His Pro Asp Ile Gln Ser 340 345 350Lys Ala Gln Glu Glu Met Asp Ala Ala Val Gly Val Arg Arg Arg Arg 355 360 365Ala Ile Thr Asp Ser Asp Val Pro Asn Leu Ala Phe Leu Gln Trp Ile 370 375 380Leu Lys Glu Thr Leu Arg Met His Pro Pro Gly Pro Leu Leu Ser Trp385 390 395 400Ala Arg Leu Ala Val Gln Asp Ala Arg Val Gly Lys His Val Val Pro 405 410 415Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ser His Asp Glu 420 425 430Ala Ile Trp Gly Asp Pro Trp Val Phe Arg Pro Glu Arg Phe Ala Ala 435 440 445Ala Ala Ala Gly Glu Glu Val Ser Val Leu Gly Ser Asp Leu Arg Leu 450 455 460Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Arg Met Met Gly465 470 475 480Leu Ala Thr Ala Gln Leu Trp Leu Gly Arg Leu Leu Gln Glu Tyr Arg 485 490 495Trp Leu Pro Pro Pro Ala Asn Lys Pro Val Glu Leu Ala Glu Cys Leu 500 505 510Arg Leu Ser Met Glu Met Lys Thr Pro Leu Val Cys Arg Ala Val Pro 515 520 525Arg Arg Arg Gly Gly Arg Pro Pro Ala Ala Ala 530 53579499PRTOryza sativa 79Met Ala Val Val Ala Leu Pro Pro Leu Leu Ala Lys Arg His Gly His1 5 10 15Ala Arg Arg Val Asn Gly Gly Gly Ala Ala Ile Pro Gly Pro Arg Gly 20 25 30Trp Pro Leu Leu Gly Ser Leu Pro Val Val Ser Gly Pro Leu Met His 35 40 45Arg Arg Leu Ala Ala Leu Ala Asp Ala His Gly Gly Gly Ala Arg Arg 50 55 60Leu Met Ser Leu Thr Leu Gly Ala Thr Pro Val Val Val Ser Ser His65 70 75 80Pro Asp Thr Val Arg Glu Ile Leu Ala Gly Ala Ala Phe Arg Asp Arg 85 90 95Pro Ala Arg Ala Ala Ala Arg Glu Leu Met Phe Leu Arg Ala Val Gly 100 105 110Phe Ala Pro Ala Ser Gly Asp Asp Gly Gly Ala Tyr Trp Arg Arg Leu 115 120 125Arg Arg Ala Ala Gly Ala Gly Met Leu Ser Pro Arg Arg Ala Ala Ala 130 135 140Leu Ala Ala Leu Arg Ala Arg Val Ala Arg Arg Thr Ser Glu Ala Val145 150 155 160Ser Arg Gly Met Ala Val Pro Pro Gly Arg Val Ala Met Arg Ala Leu 165 170 175Leu His Ala Ala Ser Leu Asp Asn Met Val Gly Ser Val Leu Gly Leu 180 185 190Glu His His Asp His His Gly Gly Val Ile Ser Asp Met Gly Asp Met 195 200 205Val Arg Glu Gly Tyr Glu Leu Val Gly Lys Phe Asn Leu Gly Asp Tyr 210 215 220Tyr Ser Thr Thr Gln Tyr Gln Cys Leu Trp Gly Leu Leu Asp Phe His225 230 235 240Gly Val Gly Pro Arg Cys Gln Arg Leu Ala Ala Arg Val Arg Glu Gln 245 250 255Phe Gly Arg Val Met Glu Glu Arg Arg Lys Val Ser Asp Leu His Lys 260 265 270Arg Asp Asp Leu Leu Ser Tyr Met Leu Ser Met Pro Gln Glu Glu Arg 275 280 285Ile Glu Asp Ser Asp Val Ile Ala Val Leu Trp Glu Met Ile Phe Arg 290 295 300Gly Thr Asp Val Val Ala Ile Leu Leu Glu Trp Ala Met Ala Arg Met305 310 315 320Val Leu His Pro Asp Ile Gln Ser Lys Val Gln Glu Glu Leu Asp Arg 325 330 335Ala Val Gly His Arg Pro Met Thr Asp Ser Asp Ile Pro Asn Leu Arg 340 345 350Phe Leu His Cys Val Ile Lys Glu Thr Leu Arg Met His Pro Pro Gly 355 360 365Pro Leu Leu Ser Trp Ala Arg Leu Ala Val His Asp Thr Tyr Val Gly 370 375 380Lys His Leu Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala385 390 395 400Ile Ser His Asp Glu Thr Ile Trp Gly Asp Pro Trp Val Phe Arg Pro 405 410 415Glu Arg Phe Met Glu Glu Asp Ile Asn Val Leu Gly Ser Asp Leu Arg 420 425 430Leu Ala Pro Phe Gly Ser Gly Arg Arg Val Cys Pro Gly Arg Met Met 435 440 445Gly Leu Ser Thr Ala Tyr Leu Trp Phe Gly Arg Met Leu Gln Glu Tyr 450 455 460Lys Trp Ala Ala Ala Gln Pro Val Lys Leu Thr Glu Cys Leu Arg Leu465 470 475 480Ser Met Glu Met Lys Lys Pro Leu Val Cys His Ala Val Pro Arg Ser 485 490 495Lys Thr Gly80555PRTOryza sativa 80Met Ala Met Ala Thr Ala Thr Ala Ser Ser Cys Val Asp Ala Thr Trp1 5 10 15Trp Ala Tyr Ala Leu Pro Ala Leu Leu Gly Ala Asp Thr Leu Cys Ala 20 25 30His Pro Ala Leu Leu Ala Gly Ala Val Leu Leu Ala Phe Ala Thr Ala 35 40 45Ala Val Leu Ala Trp Ala Ala Ser Pro Gly Gly Pro Ala Trp Ala His 50 55 60Gly Arg Gly Arg Leu Gly Ala Thr Pro Ile Glu Gly Pro Arg Gly Leu65 70 75 80Pro Val Phe Gly Ser Ile Phe Ala Leu Ser Arg Gly Leu Pro His Arg 85 90 95Ala Leu Asp Ala Met Ser Arg Asp Ala Ala Ala Pro Arg Ala Arg Glu 100 105 110Leu Met Ala Phe Ser Val Gly Glu Thr Pro Ala Val Val Ser Ser Cys 115 120 125Pro Ala Thr Ala Arg Glu Val Leu Ala His Pro Ser Phe Ala Asp Arg 130 135 140Pro Leu Lys Arg Ser Ala Arg Glu Leu Leu Phe Ala Arg Ala Ile Gly145 150 155 160Phe Ala Pro Ser Gly Glu Tyr Trp Arg Leu Leu Arg Arg Ile Ala Ser 165 170 175Thr His Leu Phe Ser Pro Arg Arg Val Ala Ala His Glu Pro Gly Arg 180 185 190Gln Ala Asp Ala Thr Ala Met Leu Ser Ala Met Ala Ala Glu Gln Ser 195 200 205Ala Thr Gly Ala Val Val Leu Arg Pro His Leu Gln Ala Ala Ala Leu 210 215 220Asn Asn Ile Met Gly Ser Val Phe Gly Arg Arg Tyr Asp Val Ser Ser225

230 235 240Ser Ser Gly Ala Ala Ala Asp Glu Ala Glu Gln Leu Lys Ser Met Val 245 250 255Arg Glu Gly Phe Glu Leu Leu Gly Ala Phe Asn Trp Ser Asp His Leu 260 265 270Pro Trp Leu Ala His Leu Tyr Asp Pro Asn His Val Ala Arg Arg Cys 275 280 285Ala Ala Leu Val Pro Arg Val Gln Ala Phe Val Arg Gly Val Ile Arg 290 295 300Asp His Arg Leu Arg Arg Asp Ser Ser Ser Thr Ala Ala Asp Asn Ala305 310 315 320Asp Phe Val Asp Val Leu Leu Ser Leu Glu Ala His Glu Asn Leu Ala 325 330 335Glu Asp Asp Met Val Ala Val Leu Trp Glu Met Ile Phe Arg Gly Thr 340 345 350Asp Thr Thr Ala Leu Val Thr Glu Trp Cys Met Ala Glu Val Val Arg 355 360 365Asn Pro Ala Val Gln Ala Arg Leu Arg Ala Glu Val Asp Ala Ala Val 370 375 380Gly Gly Asp Gly Cys Pro Ser Asp Gly Asp Val Ala Arg Met Pro Tyr385 390 395 400Leu Gln Ala Val Val Lys Glu Thr Leu Arg Ala His Pro Pro Gly Pro 405 410 415Leu Leu Ser Trp Ala Arg Leu Ala Thr Ala Asp Val Gly Leu Ala Asn 420 425 430Gly Met Val Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala 435 440 445Ile Thr His Asp Gly Glu Val Trp Ala Asp Pro Glu Ala Phe Ala Pro 450 455 460Glu Arg Phe Ile Pro Ser Glu Gly Gly Ala Asp Val Asp Val Arg Gly465 470 475 480Gly Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro 485 490 495Gly Lys Asn Leu Gly Leu Ala Thr Val Thr Leu Trp Val Ala Arg Leu 500 505 510Val His Ala Phe Asp Trp Phe Leu Pro Asp Gly Ser Pro Pro Val Ser 515 520 525Leu Asp Glu Val Leu Lys Leu Ser Leu Glu Met Lys Thr Pro Leu Ala 530 535 540Ala Ala Ala Thr Pro Arg Arg Arg Arg Ala Ala545 550 55581174PRTSorghum bicolor 81Arg Asp Gly Arg Arg Arg Gly Glu Pro Pro Leu Arg Ala Glu Arg Arg1 5 10 15Glu Gly Asp Ala Ala His Ala Pro Ala Arg Ala Arg Cys Cys Arg Gly 20 25 30Arg Ala Trp Pro Ser Thr Thr Arg Thr Ser Ala Ala Thr Leu Val Pro 35 40 45Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Ile Ala His Asp Pro 50 55 60Ala Ile Trp Ala Glu Pro Glu Glu Phe Arg Pro Glu Arg Phe Gln Glu65 70 75 80Glu Glu Glu Asp Val Ser Val Leu Gly Gly Asp Leu Arg Leu Ala Pro 85 90 95Phe Gly Ala Gly Arg Arg Val Cys Pro Asp Lys Met Leu Ala Leu Ala 100 105 110Thr Thr His Leu Trp Val Ala Gln Leu Leu His Arg Phe Glu Trp Ala 115 120 125Pro Ala Gly Ala Ala Ser Ser Gly Gly Gly Gly Val Asp Leu Ser Glu 130 135 140Arg Leu Asn Met Ser Leu Glu Met Ala Thr Pro Leu Val Cys Lys Ala145 150 155 160Val Pro Arg Ser Ala Pro Gln Leu His Ala Gly Leu Ala Ser 165 17082552PRTSorghum bicolor 82Met Ala Met Ala Ser Ala Val Ser Ser Cys Thr Asp Ser Thr Trp Trp1 5 10 15Val Tyr Ala Leu Pro Thr Leu Leu Gly Ser Asp Thr Leu Cys Ala His 20 25 30Pro Ala Leu Leu Ala Gly Leu Leu Phe Leu Thr Thr Val Thr Ala Ala 35 40 45Leu Leu Ala Trp Ala Ala Ser Pro Gly Gly Pro Ala Trp Ala His Gly 50 55 60Arg Gly Arg Leu Gly Ala Thr Pro Ile Val Gly Pro Arg Gly Leu Pro65 70 75 80Val Phe Gly Ser Ile Phe Ala Leu Ser Arg Gly Leu Pro His Arg Thr 85 90 95Leu Ala Ala Met Ala Arg Ala Ala Gly Pro Arg Ala Lys Glu Leu Met 100 105 110Ala Phe Ser Val Gly Asp Thr Pro Ala Val Val Ser Ser Cys Pro Ala 115 120 125Thr Ala Arg Glu Val Leu Ala His Pro Ser Phe Ala Asp Arg Pro Val 130 135 140Lys Arg Ser Ala Arg Glu Leu Met Phe Ala Arg Ala Ile Gly Phe Ala145 150 155 160Pro Asn Gly Glu Tyr Trp Arg Arg Leu Arg Arg Val Ala Ser Thr His 165 170 175Leu Phe Ser Pro Arg Arg Val Ala Ala His Glu Pro Gly Arg Gln Gly 180 185 190Asp Ala Glu Ala Met Leu Arg Ser Val Ala Ala Glu Gln Ser Ala Ser 195 200 205Gly Thr Val Val Leu Arg Pro His Leu Gln Ala Ala Ala Leu Asn Asn 210 215 220Ile Met Gly Ser Val Phe Gly Thr Arg Tyr Asp Val Thr Ser Gly Ala225 230 235 240Thr Ala Gly Ala Ala Glu Ala Glu Gln Leu Lys Ser Met Val Arg Glu 245 250 255Gly Phe Glu Leu Leu Gly Ala Phe Asn Trp Ser Asp His Leu Pro Trp 260 265 270Leu Ala His Leu Tyr Asp Pro Ser Asn Val Thr Arg Arg Cys Ala Ala 275 280 285Leu Val Pro Arg Val Gln Thr Phe Val Arg Gly Val Ile Asp Glu His 290 295 300Arg Arg Arg Arg Gln Asn Ser Ala Ala Leu Asp Leu Asn Asp Asn Ala305 310 315 320Asp Phe Val Tyr Val Leu Leu Ser Leu Asp Gly Asp Glu Lys Leu Arg 325 330 335Asp Asp Asp Met Val Ala Ile Leu Trp Glu Met Ile Phe Arg Gly Thr 340 345 350Asp Thr Thr Ala Leu Leu Thr Glu Trp Cys Met Ala Glu Leu Val Arg 355 360 365His Pro Ala Val Gln Ala Arg Leu Arg Ala Glu Val Asp Ala Ala Val 370 375 380Gly Ala Gly Gly Arg Pro Thr Asp Ala Asp Val Ala Arg Met Pro Tyr385 390 395 400Leu Gln Ala Val Val Lys Glu Thr Leu Arg Ala His Pro Pro Gly Pro 405 410 415Leu Leu Ser Trp Ala Arg Leu Ala Thr Ala Asp Val Pro Leu Ser Asn 420 425 430Gly Met Val Val Pro Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala 435 440 445Ile Thr His Asp Ala Gly Val Trp Ala Asp Pro Asp Ala Phe Ala Pro 450 455 460Glu Arg Phe Leu Pro Ser Glu Gly Gly Ala Asp Val Asp Val Arg Gly465 470 475 480Val Asp Leu Arg Leu Ala Pro Phe Gly Ala Gly Arg Arg Val Cys Pro 485 490 495Gly Lys Asn Leu Gly Leu Thr Thr Val Gly Leu Trp Val Ala Arg Leu 500 505 510Val His Ala Phe Glu Trp Ala Leu Pro Asp Gly Ala Pro Pro Val Cys 515 520 525Leu Asp Glu Val Leu Lys Leu Ser Leu Glu Met Lys Thr Pro Leu Ala 530 535 540Ala Ala Ala Ile Pro Arg Thr Ala545 55083561PRTBrachypodium distachyon 83Met Glu Ser Ser Val Glu Ser Ser Trp Trp Val Leu Pro Leu Thr Leu1 5 10 15Ile Pro Ala Ile Ser Gly Gln Gln Gln Gln His Asp Gln Ser Thr Ala 20 25 30Ala Ala Ile Ala Thr Ser Phe Val Tyr Leu Ala Ile Leu Ala Cys Leu 35 40 45Ala Trp Ala Ala Lys Ser Leu Leu Tyr Trp Ala His Pro Gly Gly Pro 50 55 60Ala Trp Gly Arg Arg Tyr Trp Thr Ser Pro Cys Ala Lys Thr Ala Pro65 70 75 80Ala Pro Ala Pro Ile Pro Gly Pro Arg Gly Leu Pro Val Val Gly Ser 85 90 95Leu Gly Leu Met Ser Gly Leu Ala His Ser Thr Leu Ala Ala Glu Ala 100 105 110Ala Arg Thr Pro Gly Ala Lys Arg Leu Met Ala Leu Ser Leu Gly Pro 115 120 125Val Pro Ala Val Val Thr Ala His Pro Asp Val Ala Lys Glu Ile Leu 130 135 140Asp Asn Pro Ala Phe Ala Asp Arg Pro Val Asn His Ala Ala Tyr Gly145 150 155 160Leu Met Phe His Arg Ser Ile Gly Phe Ala Glu His Gly Pro Tyr Trp 165 170 175Arg Ala Leu Arg Arg Val Ala Ser Ala His Leu Phe Ala Pro Arg Gln 180 185 190Val Asp Ala Phe Ala Pro Tyr Arg Ala Arg Val Gly Glu Asp Val Val 195 200 205Ala Ala Leu Arg His Ala Gly Gly Gly Val Val Asn Val Arg Gly Val 210 215 220Leu Arg Arg Ala Ser Leu Tyr Tyr Ile Met Arg Phe Val Phe Gly Lys225 230 235 240Glu Tyr Asp Val Ser Ser Asp Ser Gly Lys Lys Asp Gln Gly Glu Val 245 250 255Glu Glu Leu Leu Glu Met Val His Glu Gly Tyr Glu Leu Leu Gly Lys 260 265 270Glu Asn Trp Cys Asp Tyr Phe Pro Gly Leu Ala Gly Phe Asp Pro Gln 275 280 285Gly Val Gly Ala Arg Cys Ala Glu Leu Met Pro Arg Val Asn Arg Phe 290 295 300Val His Gly Ile Ile Asp Glu His Arg Gly Lys Ala Met Ile Ala Gly305 310 315 320Gly Glu Gly Glu Ala Gln Pro Leu Asp Phe Val Asp Ile Leu Leu Ser 325 330 335Leu Gln Glu Ser Glu Gly Leu Ala Asp Ala Asp Ile Ala Ala Val Leu 340 345 350Trp Glu Met Ile Phe Arg Gly Thr Asp Ala Met Ala Val Leu Met Glu 355 360 365Trp Thr Met Ala Arg Leu Val Leu His Pro Gly Val Gln Ala Asn Val 370 375 380His Lys Glu Leu Asp Glu Val Val Gly Lys Ser Ser His Val Thr Glu385 390 395 400Ser Ala Val Leu Ser Leu Pro Tyr Leu Gln Ala Leu Leu Lys Glu Ala 405 410 415Leu Arg Val His Pro Pro Gly Pro Leu Leu Ser Trp Arg His Arg Ala 420 425 430Met Trp Asp Thr Tyr Val Asp Gly His Leu Val Pro Ala Gly Thr Thr 435 440 445Ala Met Val Asn Gln Trp Ala Met Ser Arg Asp Pro Glu Val Trp Ala 450 455 460Glu Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Pro Gly Gly Glu Ala465 470 475 480Gly Pro Gly Val Ser Val Leu Gly Ser Asp Gly Arg Leu Val Pro Phe 485 490 495Gly Ser Gly Arg Arg Ser Cys Pro Gly Lys Asn Leu Ala Met Thr Thr 500 505 510Val Thr Ala Trp Met Ala Thr Leu Met His Glu Phe Glu Trp Met Pro 515 520 525Ala Lys Thr Gly Ala Pro Val Asp Met Ser Glu Val Leu Arg Leu Ser 530 535 540Cys Glu Met Ala Thr Pro Leu Gln Val Arg Val Arg Pro Arg Arg Gly545 550 555 560Val849PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motifVARIANT(8)..(8)Xaa is any amino acid, preferably Gly, His or Thr 84Gly Gly Ala Trp Gly Lys Tyr Xaa Arg1 58517PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motifVARIANT(1)..(1)Xaa is any amino acid, preferably Val or AsnVARIANT(3)..(3)Xaa is any amino acid, preferably Lys or ArgVARIANT(10)..(10)Xaa may be present or absent. If present, Xaa is any amino acid, preferably ArgVARIANT(11)..(11)Xaa is any amino acid, preferably Met or SerVARIANT(13)..(13)Xaa is any amino acid, preferably Ser, Asn or HisVARIANT(14)..(14)Xaa is any amino acid, preferably Thr or Val 85Xaa Gly Xaa Gly Val Gly Ser Met Ser Xaa Xaa Ser Xaa Xaa Ala His1 5 10 15Arg8625PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motifVARIANT(5)..(5)Xaa is any amino acid, preferably Thr or AspVARIANT(6)..(6)Xaa is any amino acid, preferably Thr or ArgVARIANT(7)..(7)Xaa may be present or absent. If present, Xaa is any amino acid, preferably LysVARIANT(8)..(8)Xaa may be present or absent. If present, Xaa is any amino acid, preferably ValVARIANT(13)..(13)Xaa is any amino acid, preferably Asn or HisVARIANT(14)..(14)Xaa is any amino acid, preferably Asp or AlaVARIANT(19)..(19)Xaa may be present or absent. If present, Xaa is any amino acid, preferably Ser 86Met Ala Ser Gly Xaa Xaa Xaa Xaa Val Val Thr Cys Xaa Xaa Val Ala1 5 10 15Lys Asn Xaa Ser Val Ala Asp Arg Val 20 258712PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motif 87Val Gly Tyr Asp Gly Thr Asn Trp Thr Asp His Trp1 5 108810PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motif 88Ala Val Trp Met Arg Gly Thr Asp Val Ala1 5 108913PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motifVARIANT(10)..(10)Xaa is any amino acid, preferably Ala or Ser 89Lys Val Arg His Gly Ser Trp Ala Arg Xaa Thr Asp Thr1 5 109049PRTArtificial sequenceSynthetic sequence CYP78A6-clade polypeptide amino acid sequence motifVARIANT(13)..(13)Xaa is any amino acid, preferably Ala or ValVARIANT(14)..(14)Xaa may be present or absent. If present, Xaa is any amino acid, preferably SerVARIANT(15)..(15)Xaa is any amino acid, preferably His or ArgVARIANT(20)..(20)Xaa is any amino acid, preferably Val, Asn or AspVARIANT(21)..(21)Xaa may be present or absent. If present, Xaa is any amino acid, preferably AspVARIANT(28)..(28)Xaa is any amino acid, preferably Ala or ValVARIANT(39)..(39)Xaa is any amino acid, preferably Arg or LysVARIANT(41)..(41)Xaa may be present or absent. If present, Xaa is any amino acid, preferably Val 90Val Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Xaa Xaa Xaa Asp1 5 10 15His Val Trp Xaa Xaa Lys Arg Val Ala Lys Gly Xaa Ser Val Gly Ser 20 25 30Asp Arg Ala Gly Ser Gly Xaa Arg Xaa Cys Gly Lys Asn Gly Thr Thr 35 40 45Val9117PRTArabidopsis thaliana 91Gly Gly Ala Trp Gly Lys Tyr Gly Arg Ser Gly Ser Tyr Lys Thr Gly1 5 10 15Asn9216PRTArabidopsis thaliana 92Val Gly Lys Gly Val Gly Ser Met Ser Met Ser Ser Thr Ala His Arg1 5 10 159322PRTArabidopsis thaliana 93Met Ala Ser Gly Thr Arg Val Val Thr Cys Asn Asp Val Ala Lys Asn1 5 10 15Ser Val Ala Asp Arg Val 209413PRTArabidopsis thaliana 94Lys Val Arg His Gly Ser Trp Ala Arg Ala Thr Asp Thr1 5 109548PRTArabidopsis thaliana 95Val Ala Gly Thr Thr Ala Met Val Asn Met Trp Ala Val Ser His Asp1 5 10 15His Val Trp Val Asp Lys Arg Val Ala Lys Gly Val Ser Val Gly Ser 20 25 30Asp Arg Ala Gly Ser Gly Arg Arg Cys Gly Lys Asn Gly Thr Thr Val 35 40 45961229DNAArabidopsis thaliana 96acactctttc ctctctcttt cttctctctt tcttttctct ctctctcctc tgctcctccg 60tctctcgtct acagtgccct ccgcatcacc tttttccttg tcctatgaat ttggtcgaaa 120tgcccttctc ctcctcctcc ttccactaat ctcaaaagat atatccttcg agactctccc 180ttgccgtctc caattgccac tcaccgctcc aactctcttc gaattagctg aaatgaatgg 240agataataga ccagtggaag atgctcatta cacggagaca ggtttccctt atgctgctac 300tggaagttac atggactttt atggtggtgc ggctcagggg cctcttaact acgatcatgc 360cgcaactatg catcctcagg acaatctgta ctggaccatg aataccaatg catacaagtt 420tgggttttca ggatcagata atgcttcttt ctatggttca tatgacatga acgatcattt 480atcgaggatg tccataggga gaacaaattg ggactatcat cccatggtga acgttgctga 540tgatcctgaa aacacagttg cacgttccgt ccaaatcgga gacacagatg agcactctga 600agctgaagaa tgcattgcaa atgagcatga tcccgacagt cctcaggtat cctggcaaga 660tgacattgat cctgatacaa tgacctatga ggaattagta gagctggggg aagcagtagg 720aacagaaagc agggggttgt ctcaggaact catagaaacg cttcccacta aaaagtataa 780gtttgggagc atcttctcca ggaaaagagc tggggagagg tgtgtgatat gccagctcaa 840gtacaagata ggggagaggc aaatgaatct gccgtgcaag catgtgtatc attctgaatg 900catttccaaa tggctaagca tcaacaaggt ttgcccggtg tgtaacagcg aggtctttgg 960ggagcccagc attcattgat cggcacaagg ggctcctcct cttcttttct ttttggcttt 1020ttatatcgag gctcatcaag taattgtttt agtgtagtga aaaccccaaa aaatagtcta 1080aaagatgtcc acactatact ctctcatgtt cagtccttct ctgtacatgt aatttttctt 1140ctagttccat tttcgcttgt gtgtgcttta agtttaacag tcactcgtat tgtatactaa 1200atgctaagtc aaaaacgctg aatccatat 122997248PRTArabidopsis thaliana 97Met Asn Gly Asp Asn Arg Pro Val Glu Asp Ala His Tyr Thr Glu Thr1 5 10 15Gly Phe Pro

Tyr Ala Ala Thr Gly Ser Tyr Met Asp Phe Tyr Gly Gly 20 25 30Ala Ala Gln Gly Pro Leu Asn Tyr Asp His Ala Ala Thr Met His Pro 35 40 45Gln Asp Asn Leu Tyr Trp Thr Met Asn Thr Asn Ala Tyr Lys Phe Gly 50 55 60Phe Ser Gly Ser Asp Asn Ala Ser Phe Tyr Gly Ser Tyr Asp Met Asn65 70 75 80Asp His Leu Ser Arg Met Ser Ile Gly Arg Thr Asn Trp Asp Tyr His 85 90 95Pro Met Val Asn Val Ala Asp Asp Pro Glu Asn Thr Val Ala Arg Ser 100 105 110Val Gln Ile Gly Asp Thr Asp Glu His Ser Glu Ala Glu Glu Cys Ile 115 120 125Ala Asn Glu His Asp Pro Asp Ser Pro Gln Val Ser Trp Gln Asp Asp 130 135 140Ile Asp Pro Asp Thr Met Thr Tyr Glu Glu Leu Val Glu Leu Gly Glu145 150 155 160Ala Val Gly Thr Glu Ser Arg Gly Leu Ser Gln Glu Leu Ile Glu Thr 165 170 175Leu Pro Thr Lys Lys Tyr Lys Phe Gly Ser Ile Phe Ser Arg Lys Arg 180 185 190Ala Gly Glu Arg Cys Val Ile Cys Gln Leu Lys Tyr Lys Ile Gly Glu 195 200 205Arg Gln Met Asn Leu Pro Cys Lys His Val Tyr His Ser Glu Cys Ile 210 215 220Ser Lys Trp Leu Ser Ile Asn Lys Val Cys Pro Val Cys Asn Ser Glu225 230 235 240Val Phe Gly Glu Pro Ser Ile His 2459822DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 98ggtctaagat ttctctcgtg tc 229922DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 99cgtacgtctt ctattactcc ac 2210021DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 100aactccaaag gatcaaccca c 2110121DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 101ccggttaaag aatcggctta c 2110221DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 102gacttgcaaa gatcgttcac c 2110321DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 103actcaatgtg acgtgttgtg g 2110421DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 104tttgatcgag tggattcttg c 2110521DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 105atatttgctt gtaatcgggg c 2110621DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 106taaaaccaaa cgacaccgtt c 2110721DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 107tccaagtttg ttgacgattc c 2110824DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 108cgagtatcaa tggaaactta accg 2410920DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 109aacggagagt ggcttgagat 2011020DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 110tggcccttat ggtttctgca 2011115DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNAmisc_feature(1)..(1)n is a or g or c or t 111ntcgastwts gwgtt 1511222DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 112tggttcacgt agtgggccat cg 2211334DNAArtificial sequenceSynthetic sequence Primer for verifying T-DNA 113gcttcctatt atatcttccc aaattaccaa taca 3411430DNAArtificial sequenceSynthetic sequence Primer for constructs 114ctgcagatgg ctacgaaact cgaaagctcc 3011530DNAArtificial sequenceSynthetic sequence Primer for constructs 115ctgcagttaa ctgcgcctac ggcgcaattt 3011624DNAArtificial sequenceSynthetic sequence Primer for constructs 116gagctctgtc tcgtggataa gtag 2411727DNAArtificial sequenceSynthetic sequence Primer for constructs 117ccatggggcg gatcaaagca aagtaag 2711818DNAArtificial sequenceSynthetic sequence Primer for RT-PCR 118accaaccttg ccttctcc 1811919DNAArtificial sequenceSynthetic sequence Primer for RT-PCR 119cgtctcggct cttctgatt 1912017DNAArtificial sequenceSynthetic sequence Primer for RT-PCR 120acaacgagca gcaacca 1712119DNAArtificial sequenceSynthetic sequence Primer for RT-PCR 121tcttcaaccg gaacttcat 1912218DNAArtificial sequenceSynthetic sequence Primer for RT-PCR 122atccttcctg atatcgac 1812318DNAArtificial sequenceSynthetic sequence Primer for RT-PCR 123gagaagatga ctcagatc 1812420DNAArtificial sequenceSynthetic sequence Primer for quantitative real-time RT-PCR 124ccggttaaag aatcggctta 2012520DNAArtificial sequenceSynthetic sequence Primer for quantitative real-time RT-PCR 125ttgagatcac tcgtcgttgc 2012620DNAArtificial sequenceSynthetic sequence Primer for quantitative real-time RT-PCR 126gaaatcacag cacttgcacc 2012720DNAArtificial sequenceSynthetic sequence Primer for quantitative real-time RT-PCR 127aagcctttga tcttgagagc 2012822DNAArtificial sequenceSynthetic sequence Primer for in situ hybridization 128aaagaagctc atatgagaat ta 2212923DNAArtificial sequenceSynthetic sequence Primer for in situ hybridization 129tggtgtaaat ataaattgaa act 2313024DNAArtificial sequenceSynthetic sequence Primer for in situ hybridization 130ttagtgtatg ataaggctaa ggct 2413122DNAArtificial sequenceSynthetic sequence Primer for in situ hybridization 131gtattaactt ttctttgtga ca 22

Claims

1. A method of modulating seed size in a plant comprising reducing or abolishing expression of a CYP78A polypeptide within cells of said plant, wherein said reduced or abolished expression reduces seed size in said plant.

2. A method according to claim 1 comprising expressing a heterologous nucleic encoding an RNA molecule which suppresses expression of a CYP78A polypeptide within cells of said plant.

3. A method according to claim 2 comprising mutating a nucleic acid coding sequence encoding a CYP78A polypeptide within cells of said plant or a nucleic acid which regulates the expression of said nucleic acid coding sequence.

4. A method according to claim 3 comprising mutating a nucleic acid which regulates the expression of said nucleic acid coding sequence.

5. A method of producing a plant with an altered seed size comprising: incorporating a heterologous nucleic acid which alters the expression of a CYP78A polypeptide into a plant cell by means of transformation, and; regenerating the plant from one or more transformed cells.

6. A method according to claim 5 wherein the heterologous nucleic acid inactivates an element which represses expression of a CYP78A6 polypeptide.

7. A method according to claim 5 wherein the heterologous nucleic acid reduces expression of the CYP78A polypeptide and the seed size of the plant is reduced.

8. A method according to claim 7 wherein the heterologous nucleic acid encodes an RNA molecule which suppresses a CYP78A polypeptide.

9. A method according to claim 7 wherein the heterologous nucleic acid inactivates the expression of a CYP78A polypeptide.

10. A method according to claim 1 further comprising incorporating a heterologous nucleic acid which expresses a suppressor nucleic acid which reduces expression of a DA polypeptide into said plant cell by means of transformation or expressing a heterologous nucleic acid which encodes suppressor nucleic acid which reduces expression of a DA polypeptide in said plant.