PLANTS WITH INCREASED SEED SIZE

Abstract

The invention relates to genetically modified plants with an altered seed phenotype, in particular increased seed size. The invention relates to a plant that does not produce a functional NGAL2 polypeptide or functional NGAL2 and NGAL3 polypeptides. NGAL2 and NGAL3 are members of the RAV family and comprise a B3 DNA-binding domain and a transcriptional repression motif.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This is a continuation application of U.S. Ser. No. 15/548,398 filed Aug. 2, 2017, which is a National Phase application claiming priority to PCT/GB2016/050245, filed Feb. 3, 2016, which claims priority to PCT/CN2015/072143, filed Feb. 3, 2015, all of which are herein incorporated by reference in their entirety.

FIELD OF THE INVENTION

[0002] The invention relates to transgenic plants with improved growth and yield-related traits, in particular increased seed size. Also within the scope of the invention are related methods, uses, isolated nucleic acids and vector constructs.

INTRODUCTION

[0003] The ever-increasing world population and the dwindling supply of arable land available for agriculture fuels research towards increasing the efficiency of agriculture and providing food security. Conventional means for crop and horticultural improvements utilise selective breeding techniques to identify plants having desirable characteristics. However, such selective breeding techniques have several drawbacks, namely that these techniques are typically labour intensive and result in plants that often contain heterogeneous genetic components that may not always result in the desirable trait being passed on from parent plants. Advances in molecular biology have allowed mankind to modify the germplasm of animals and plants. Genetic engineering of plants entails the isolation and manipulation of genetic material (typically in the form of DNA or RNA) and the subsequent introduction of that genetic material into a plant. Such technology has the capacity to deliver crops or plants having various improved economic, agronomic or horticultural traits, including increased yield. There are a number of methods that can be used, for example genome editing (using CRISPR or TALEN) or mutagenesis.

[0004] A trait of particular economic interest is increased seed size. Seed size is an important agronomic trait which increased crop yield, and is also a key ecological trait that influences many aspects of a species' regeneration strategy, such as seedling survival rates and seed dispersal syndrome (Harper et al., 1970; Westoby et al., 2002; Moles et al., 2005; Fan et al., 2006; Orsi and Tanksley, 2009; Gegas et al., 2010). Although the size of seeds is one of the most important agronomic traits in plants, the genetic and molecular mechanisms that set the final size of seeds are almost unknown. In higher plants, seed development starts with a double fertilization process, in which one of the two haploid pollen nuclei fuses with the haploid egg cell to produce the diploid embryo, while the other sperm nucleus fuses with the diploid central cell to form the triploid endosperm (Lopes and Larkins, 1993). The integuments surrounding the ovule are maternal tissues and form the seed coat after fertilization. Therefore, the size of the seed is the result of the growth of the embryo, the endosperm and the maternal tissues. However, the genetic and molecular mechanisms setting the limits of seed growth are almost unknown in plants.

[0005] Several factors that function maternally to regulate seed size have been identified in Arabidopsis. For example, TRANSPARENT TESTA GLABRA 2 (TTG2) influences seed growth by increasing cell elongation in the maternal integuments (Garcia et al., 2005; Ohto et al., 2009), while APETALA2 (AP2) may control seed growth by limiting cell elongation in the maternal integuments (Jofuku et al., 2005; Ohto et al., 2005; Ohto et al., 2009). By contrast, AUXIN RESPONSE FACTOR 2 (ARF2) acts maternally to control seed growth by restricting cell proliferation (Schruff et al., 2006). Similarly, the ubiquitin receptor DA1 acts synergistically with the E3 ubiquitin ligases DA2 and EOD1/BB to control seed size by limiting cell proliferation in the maternal integuments (Li et al., 2008; Xia et al., 2013). Mutations in the suppressor of da1-1 (SOD2), which encodes the ubiquitin-specific protease (UBP15), suppress the large seed phenotype of da1-1 (Du et al., 2014). DA1 physically associates with UBP15/SOD2 and modulates the stability of UBP15. These studies show that the ubiquitin pathway plays an important part in the maternal control of seed size. KLU/CYTOCHROME P450 78A5 (CYP78A5) regulates seed size by increasing cell proliferation in the maternal integuments of ovules (Adamski et al., 2009). KLU has also been suggested to generate mobile plant-growth substances that promote cell proliferation (Anastasiou et al., 2007; Adamski et al., 2009). By contrast, overexpression of CYP78A6/EOD3 increases both cell proliferation and cell elongation in the integuments, resulting in large seeds (Fang et al., 2012). Seed size is also determined by zygotic tissues. Several factors have been described to influence seed size via the zygotic tissues in Arabidopsis, including HAIKU1(IKU1), IKU2, MINISEED3 (MINI3) and SHORT HYPOCOTYL UNDER BLUE1 (SHB1) (Garcia et al., 2003; Luo et al., 2005; Zhou et al., 2009; Wang et al., 2010; Kang et al., 2013). iku and mini3 mutants form small seeds due to precocious cellularization of the endosperm (Garcia et al., 2003; Luo et al., 2005; Wang et al., 2010). SHB1 associates with MINI3 and IKU2 promoters and regulates expression of MINI3 and IKU2 (Zhou et al., 2009; Kang et al., 2013). ABA INSENSITIVE5 (AB15) has been recently described to repress the expression of SHB1 (Cheng et al., 2014), and MINI3 has been reported to activate expression of the cytokinin oxidase (CKX2) (Li et al., 2013), suggesting the roles of phytohormones in regulating endosperm growth. In addition, the endosperm growth is influenced by parent of-origin effects (Scott et al., 1998; Xiao et al., 2006).

[0006] The invention is aimed at providing plants with improved yield traits that are beneficial to agriculture.

SUMMARY OF THE INVENTION

[0007] In a first aspect, the invention relates to a plant generated that does not produce a functional NGAL2 polypeptide or does not produce functional NGAL2 and NGAL3 polypeptides.

[0008] In another aspect, the invention relates to a method for altering a plant phenotype comprising reducing or abolishing the expression of a nucleic acid sequence encoding a NGAL2 polypeptide or reducing or abolishing the activity of a NGAL2 or reducing or abolishing the expression of a nucleic acid sequences encoding NGAL2 and NGAL3 polypeptides or reducing or abolishing the activity of a NGAL2 and NGAL3 polypeptide relative to a control plant.

[0009] In another aspect, the invention relates to a method for making a plant with an altered phenotype comprising reducing or abolishing the expression of a nucleic acid sequence encoding a NGAL2 polypeptide or reducing or abolishing the activity of a NGAL2 or reducing or abolishing the expression of a nucleic acid sequences encoding NGAL2 and NGAL3 polypeptides or reducing or abolishing the activity of a NGAL2 and NGAL3 polypeptide relative to a control plant.

[0010] In another aspect, the invention relates to a plant obtained or obtainable any method described above.

[0011] In another aspect, the invention relates to an isolated nucleic acid comprising a sequence comprising or consisting of SEQ ID NO: 1 or 2 or a functional variant or homologue thereof.

[0012] In another aspect, the invention relates to a vector comprising an isolated nucleic acid described above.

[0013] In another aspect, the invention relates to a silencing nucleic acid construct targeting sequence comprising or consisting of

[0014] 1, 2 or 3 or a functional variant, part or homologue thereof.

FIGURES

[0015] The invention is further described in the following non-limiting figures.

[0016] FIG. 1. Isolation of a suppressor of da1-1 (sod7-1).

[0017] (A) Seeds from wild-type, da1-1 and sod7-1D da1-1 plants (from left to right). (B) Mature embryos of the wild type, da1-1 and sod7-1D da1-1 (from left to right). (C) Flowers from wild-type, da1-1 and sod7-1D da1-1 plants (from left to right). (D) 30-day-old plants of the wild type, da1-1 and sod7-1D da1-1 (from left to right). (E) Projective area of wild-type, da1-1 and sod7-1D da1-1 seeds. (F) Weight of wild-type, da1-1 and sod7-1D da1-1 seeds. (G) Cotyledon area of 10-d-old wild-type, da1-1 and sod7-1D da1-1 seedlings. Values (E-G) are given as mean.+-.SD relative to the respective wild-type values, set at 100%. **, P<0.01 compared with da1-1 (Student's t-test). Bars=0.5 mm in (A), 0.2 mm in (B), 1 mm in (C) and 5 cm in (D).

[0018] FIG. 2. Seed and organ size in the sod7-1D mutant.

[0019] (A and B) Seeds of Col-0 (A) and sod7-1D (B). (C and D) Mature embryos of Col-0 (C) and sod7-1D (D). (E and F) 10-day-old seedlings of Col-0 (E) and sod7-1D (F). (G) Projective area of Col-0 and sod7-1D seeds. (H) Weight of Col-0 and sod7-1D seeds. (I) Cotyledon area of 10-day-old Col-0 and sod7-1D seedlings. Values (G-I) are given as mean.+-.SD relative to the respective wild-type values, set at 100%. **, P<0.01 compared with the wild type (Student's t-test). Bars=0.5 mm in (A) and (B), 0.2 mm in (C) and (D), and 1 mm in (E) and (F).

[0020] FIG. 3. Cloning of the SOD7 gene.

[0021] (A) Structure of the T-DNA insertion in the sod7-1D mutant. (B) Expression levels of At3g11580 (SOD7) and At3g11590 in da1-1 and sod7-1D da1 seedlings.

[0022] (C) The SOD7 protein contains a B3 DNA binding domain (second domain in lighter shading) and a transcriptional repression motif (small light box in darker shading, marked with an arrow). (D) Projective area of Col-0, 35S:GFP-SOD7#3 and 35S:GFP-SOD7#5 seeds. (E) Cotyledon area of 10-day-old Col-0, 35S:GFP-SOD7#3 and 35S:GFP-SOD7#5 seedlings. (F) Expression levels of SOD7 in Col-0, 35S:GFP-SOD7#3 and 35S:GFP-SOD7#5 seedlings. Values (D-F) are given as mean.+-.SD relative to the respective wild-type values, set at 100%. **, P<0.01 compared with the wild type (Student's t-test).

[0023] FIG. 4. Expression pattern and subcellular localization of SOD7.

[0024] (A-K) SOD7 expression activity was monitored by pSOD7:GUS transgene expression. Histochemical analysis of GUS activity in the developing leaves (A, B and C), the developing sepals (D, E), the developing petals (F, G), the developing stamens (H, I), and the developing carpels (J, K). (L) GFP florescence of SOD7-GFP in a young ovule of pSOD7:SOD7-GFP transgenic plants. (M-O) GFP fluorescence of SOD7-GFP (M), DAPI staining (N), and merged (0) images are shown. Epidermal cells in pSOD7:SOD7-GFP leaves were used to observe GFP signal. (P-R) GFP fluorescence of GFP-SOD7 (P), DAPI staining (Q), and merged (R) images are shown. Epidermal cells in 35S:GFP-SOD7 leaves were used to observe GFP signal. Bars=100 .mu.m in (A-K), 10 .mu.m in (L), and 2 .mu.m in (M-R).

[0025] FIG. 5. SOD7 acts redundantly with NGAL3 to control seed size.

[0026] (A) The SOD7 gene structure. The start codon (ATG) and the stop codon (TGA) are shown. Closed boxes indicate the coding sequence, and the line between boxes indicates intron. The T-DNA insertion site (sod7-ko1) in the SOD7 gene was indicated.

[0027] (B) The NGAL3 gene structure. The start codon (ATG) and the stop codon (TGA) are shown. Closed boxes indicate the coding sequence, and the line between boxes indicates intron. The T-DNA insertion site (ngal3-ko1) in the NGAL3 gene was indicated. (C) Seeds from Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 plants (from left to right). (D) Mature embryos of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 (from left to right). (E) 25-day-old plants of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 (from left to right). (F) Flowers of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 (from left to right). (G) Projective area of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 seeds. (H) Weight of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 seeds. (I) Cotyledon area of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1 seedlings. Values (G-I) are given as mean.+-.SD relative to the respective wild-type values, set at 100%. **, P<0.01 compared with the wild type (Col-0) (Student's t-test). Bars=0.5 mm in (C), 0.2 mm in (D), 5 cm in (E), and 1 mm in (F).

[0028] FIG. 6. SOD7 acts maternally to determine seed size.

[0029] (A) Projective area of Col-0.times.Col-0 (C/C) F1, Col-0.times.sod7-ko1 ngal3-ko1 (C/d) F1, sod7-ko1 ngal3-ko1.times.Col-0 (d/C) F1 and sod7-ko1 ngal3-ko1.times.sod7-ko1 ngal3-ko1 (d/d) F1 seeds. Values are given as mean.+-.SD relative to the respective wild-type values, set at 100%. (B) Projective area of Col-0.times.Col-0 (C/C) F2, Col-0.times.sod7-ko1 ngal3-ko1 (C/d) F2, sod7-ko1 ngal3-ko1.times.Col-0 (d/C) F2 and sod7-ko1 ngal3-ko1.times.sod7-ko1 ngal3-ko1 (d/d) F2 seeds. Values are given as mean.+-.SD relative to the respective wild-type values, set at 100%. (C and D) Mature ovules of Col-0 (C) and sod7-ko1 ngal3-ko1 (D). (E) Outer integument length of mature Col-0 (lighter bar to the left) and sod7-ko1 ngal3-ko1 (darker bar to the right) ovules. Values are given as mean.+-.SD. (F) The number of cells in the outer integuments of Col-0 and sod7-ko1 ngal3-ko1 at 0, 6 and 8 DAP. Values are given as mean.+-.SD. (F) The length of cells in the outer integuments of Col-0 and sod7-ko1 ngal3-ko1 at 0, 6 and 8 DAP. Values are given as mean.+-.SD. **, P<0.01 compared with the wild type (Col-0) (Student's t-test). Bars=50 .mu.m in (C) and (D).

[0030] FIG. 7. klu-4 is epistatic to sod7-ko1 ngal3-ko1 with respect to seed size.

[0031] (A) Seed area of Col-0, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 (from left to right). Values are given as mean.+-.SD relative to the respective wild-type values, set at 100%. (B) Seed weight of Col-0, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 (from left to right). Values are given as mean.+-.SD relative to the respective wild-type values, set at 100%. (C) The outer integument length of Col-0, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 (from left to right). ngal3-ko1 at 0 and 8 DAP. Values are given as mean.+-.SD. (D) The number of cells in the outer integuments of Col-0, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 (from left to right) at 0 and 8 DAP. Values are given as mean.+-.SD. **, P<0.01 compared with their respective controls (Student's t-test).

[0032] FIG. 8. SOD7 directly binds to the promoter of KLU and represses the expression of KLU.

[0033] (A) Expression dynamics of SOD7 and KLU in pER8-SOD7 transgenic plants treated with .beta.-estradiol for 0, 4 and 8 hours. Means were calculated from three biological samples. Values are given as mean.+-.SD. **, P<0.01, compared with the expression level of KLU and SOD7 at 0 hour, respectively (Student's t-test). (B) A 2-kb promoter region of KLU upstream of its ATG codon contains a CACTTG sequence. PF1 and PF2 represent PCR fragments used for ChIP-quantitative PCR analysis. A and A-m indicate the wild-type probe and the mutated probe used in the EMSA essay, respectively. (C) ChIP-qPCR analysis shows that SOD7 binds to the promoter fragment PF1 of KLU. Chromatin from 35S:GFP and 35S:GFP-SOD7 transgenic plants was immunoprecipitated by anti-GFP, and the enrichment of the fragments was determined by quantitative real-time PCR. The ACTIN7 promoter was used as a negative control. The fold enrichment was normalized to the ACTIN7 amplicon, set at 1. Means were calculated from three biological samples. Values are given as mean.+-.SD. **, P<0.01, compared with 35S:GFP transgenic plants (Student's t-test). (D) Direct interaction between SOD7 and the KLU promoter determined by EMSA. The biotin-labeled probe A and MBP-SOD7 formed the DNA-protein complex, but the mutated probe A-m and MBP-SOD7 did not form the DNA-protein complex. The retarded DNA-protein complex was reduced by competition using the unlabeled probe A.

[0034] FIG. 9. The organ size phenotype of 35S:GFP-SOD7 transgenic plants.

[0035] Overexpression of SOD7 results in small plants compared with the wild type. Bar=5 cm.

[0036] FIG. 10. Phylogenetic tree of the RAV family members in Arabidopsis.

[0037] FIG. 11. SOD7 acts redundantly with NGAL3 to influence organ size.

[0038] Petal area of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1. (B) The seventh leaf area of Col-0, sod7-ko1, ngal3-ko1 and sod7-ko1 ngal3-ko1. Values (A and B) are given as mean.+-.SD relative to the respective wild-type values, set at 100%. **, P<0.01 and *, P<0.05 compared with the wild type (Col-0).

[0039] FIG. 12: Conserved domains in NGAL2, NGAL3 and homologs. a) B box motif. b) Repressor motif

[0040] FIG. 13: Alignment of sequences. The following sequences are shown (from top to bottom): RMZM2G053008, HvMLOC_57250, Os12g0157000, GmLoc100778733, Bra004501, Bra000434, Bra040478, Bra014415, Bra003482, Bra007646, GmLoc100781489, GRMZM2G024948_T01, os02g0683500, HvMLOC_66387, os04g0581400, GRMZM2G102059_T01, os10g0537100, GRMZM2G142999_T01, GRMZM2G125095_T01, os03g0120900, GRMZM2G098443_T01, GRMZM2G082227_T01, Os11g0156000, GRMZM2G328742_T01, GmLoc100802734 GmLoc100795470, GmLoc100818164, Bra017262, At2g36080/NGAL1, Bra005301, At3g11580/SOD7, BraLOC103849927, Bra034828, At5g06250/NGAL3, Bra005886, GmLoc102660503, HvMLOC_38822, os01g0693400, HvMLOC44012, HvMLOC_7940 HvMLOC_75135, TRAECDM81004, HvMLOC_56567, TRAES3BF098300010CFD21 HvMLOC_63261, TRAES3BF062700040CFD21, TRAES3BF062600010CFD21, Bra038346, GmLoc732601, GmLoc100789009, GmLoc100776987, GmLoc100801107. Conserved B3 domain and repressor motif are boxed.

[0041] FIG. 14: Genome editing experiments to knock out rice genes Os11g01560000 and Os12g0157000 in rice. gRNA stands for guide RNA, target site linked with gRNA scaffold will recruit CAS9 enzyme to target site in the genome and cause gene-editing.

DETAILED DESCRIPTION

[0042] The present invention will now be further described. In the following passages, different aspects of the invention are defined in more detail. Each aspect so defined may be combined with any other aspect or aspects unless clearly indicated to the contrary. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous.

[0043] The practice of the present invention will employ, unless otherwise indicated, conventional techniques of botany, microbiology, tissue culture, molecular biology, chemistry, biochemistry and recombinant DNA technology, bioinformatics which are within the skill of the art. Such techniques are explained fully in the literature.

[0044] As used herein, the words "nucleic acid", "nucleic acid sequence", "nucleotide", "nucleic acid molecule" or "polynucleotide" are intended to include DNA molecules (e.g., cDNA or genomic DNA), RNA molecules (e.g., mRNA), naturally occurring, mutated, synthetic DNA or RNA molecules, and analogues of the DNA or RNA generated using nucleotide analogues. It can be single-stranded or double-stranded. Such nucleic acids or polynucleotides include, but are not limited to, coding sequences of structural genes, anti-sense sequences, and non-coding regulatory sequences that do not encode mRNAs or protein products. These terms also encompass a gene. The term "gene" or "gene sequence" is used broadly to refer to a DNA nucleic acid associated with a biological function. Thus, genes may include introns and exons as in the genomic sequence, or may comprise only a coding sequence as in cDNAs, and/or may include cDNAs in combination with regulatory sequences.

[0045] The terms "peptide", "polypeptide" and "protein" are used interchangeably herein and refer to amino acids in a polymeric form of any length, linked together by peptide bonds.

[0046] For the purposes of the invention, "transgenic", "transgene" or "recombinant" means with regard to, for example, a nucleic acid sequence, an expression cassette, gene construct or a vector comprising the nucleic acid sequence or an organism transformed with the nucleic acid sequences, expression cassettes or vectors according to the invention, all those constructions brought about by recombinant methods in which either

[0047] (a) the nucleic acid sequences encoding proteins useful in the methods of the invention, or

[0048] (b) genetic control sequence(s) which is operably linked with the nucleic acid sequence according to the invention, for example a promoter, or

[0049] (c) both (a) and (b)

[0050] are not located in their natural genetic environment or have been modified by genetic intervention techniques, it being possible for the modification to take the form of, for example, a substitution, addition, deletion, inversion or insertion of one or more nucleotide residues. The natural genetic environment is understood as meaning the natural genomic or chromosomal locus in the original plant or the presence in a genomic library. In the case of a genomic library, the natural genetic environment of the nucleic acid sequence is preferably retained, at least in part. The environment flanks the nucleic acid sequence at least on one side and has a sequence length of at least 50 bp, preferably at least 500 bp, especially preferably at least 1000 bp, most preferably at least 5000 bp. A naturally occurring expression cassette--for example the naturally occurring combination of the natural promoter of the nucleic acid sequences with the corresponding nucleic acid sequence encoding a polypeptide useful in the methods of the present invention, as defined above--becomes a transgenic expression cassette when this expression cassette is modified by non-natural, synthetic ("artificial") methods such as, for example, mutagenic treatment. Suitable methods are described, for example, in U.S. Pat. No. 5,565,350 or WO 00/15815 both incorporated by reference.

[0051] In certain embodiments, a transgenic plant for the purposes of the invention is thus understood as meaning, as above, that the nucleic acids used in the method of the invention are not at their natural locus in the genome of said plant, it being possible for the nucleic acids to be expressed homologously or heterologously. Thus, the plant can express a silencing construct transgene. However, as mentioned, in certain embodiments, transgenic also means that, while the nucleic acids according to the different embodiments of the invention are at their natural position in the genome of a plant, the sequence has been modified with regard to the natural sequence, and/or that the regulatory sequences of the natural sequences have been modified, for example by mutagenesis.

[0052] Transgenic is preferably understood as meaning the expression of the nucleic acids according to the invention at an unnatural locus in the genome, i.e. homologous or, preferably, heterologous expression of the nucleic acids takes place. According to the invention, the transgene is stably integrated into the plant and the plant is preferably homozygous for the transgene.

[0053] The various aspects of the invention use genetic engineering methods. Thus, the plants have been generated using genetic engineering methods, for example transgene expression, mutagenesis, gene targeting, gene silencing or genome editing as detailed below. Thus, the various aspects of the invention can involve recombinant DNA technology. The plants of the invention are thus mutant plants which have been genetically engineered, that is manipulated by human intervention. The plants of the various aspects of the invention do not relate to natural variants which have not been manipulated by genetic engineering methods. The plant may be a transgenic plant in some embodiments, for example a plant which comprises a nucleic acid construct expressing a silencing construct.

[0054] In preferred embodiments exclude embodiments that are solely based on generating plants by traditional breeding methods.

[0055] The inventor has identified a B3 domain transcriptional repressor termed AtNGAL2, encoded by the suppressor of Atda1-1 (AtSOD7), which acts maternally to control seed size by restricting cell proliferation in the integuments of ovules and developing seeds.

[0056] The inventor previously identified the ubiquitin receptor DA1 as a negative regulator of seed size in Arabidopsis (Li et al., 2008). The da1-1 mutant formed large seeds due to increased cell proliferation in the maternal integuments (Li et al., 2008; Xia et al., 2013). To identify novel components in the DA1 pathway or other seed size regulators, the inventor initiated a T-DNA activation tagging screen for modifiers of da1-1 (Fang et al., 2012). A dominant suppressor of da1-1 (sod7-1D) was isolated from seeds produced from approximate 16,000 T1 plants (FIG. 1A). Seeds of the sod7-1D da1-1 double mutant were significantly smaller and lighter than da1-1 seeds (FIGS. 1A, E and F). The results show that the sod7-1D mutation suppressed the seed and organ size phenotypes of da1-1. The SOD7 gene was isolated and found to encode a NGATHA like protein (NGAL2) containing a B3 DNA-binding domain and a transcriptional repression motif (FIG. 3C) (Alvarez et al., 2009; Ikeda and Ohme-Takagi, 2009; Trigueros et al., 2009). SOD7 belongs to the RAV gene family that consists of 13 members in Arabidopsis (FIG. 10) (Swaminathan et al., 2008). Several members of the RAV family contain the putative transcriptional repression motifs, including NGA1, NGA2, NGA3, NGA4, NGAL1, NGAL2/SOD7 and NGAL3 (FIG. 10) (Ikeda and Ohme-Takagi, 2009). The transcriptional repression motifs in NGA1, NGAL1 and NGAL2/SOD7 have been known to possess the repressive activity (Ikeda and Ohme-Takagi, 2009), indicating that they are transcriptional repressors. SOD7 exhibits the highest similarity to Arabidopsis NGAL3/DEVELOPMENT-RELATED PcG TARGET IN THE APEX 4 (DPA4) (FIG. 10), which has known roles in the regulation of leaf serrations (Engelhorn et al., 2012), but no previously identified function in seed size control.

[0057] The inventor has shown that overexpression of AtSOD7 significantly decreases seed size of wild-type plants, while the disruption of AtSOD7 increases seed size. The inventors have shown that disruption of AtNGAL3, a close homolog of AtSOD7 also increases seed size. Moreover, the simultaneous disruption of AtSOD7 and AtNGAL3 further increases seed size in a synergistic manner. Genetic analyses carried out by the inventor indicate that AtSOD7 acts in a common pathway with the seed size regulator AtKLU to control seed growth, but does so independently of AtDA1. Further results show that AtSOD7 directly binds to the promoter of AtKLU in vitro and in vivo and represses expression of AtKLU. Therefore, the inventor's findings show that AtSOD7 (aka AtNGAL2) is a target for seed size improvement in crops. The plants of the invention are characterised by increased organ size, for example increased seed size, and also increased petal size, increased embryo size, for example. Increased seed size leads to an increase in seed yield and the plants of the invention are thus characterised by increased seed yield.

[0058] Thus, the invention relates to a plant wherein said plant does not produce a functional NGAL2 and/or NGAL3 polypeptide. For example, the plant does not produce a full length transcript of a nucleic acid sequence encoding a NGAL2 and/or NGAL3 protein. In another embodiment, the plant produces a full length transcript of a nucleic acid sequence encoding a NGAL2 and/or NGAL3, but the resulting protein is not functional. In a preferred embodiment, said plant does not produce a functional NGAL2 polypeptide and also does not produce a functional NGAL3 polypeptide. Such plants are double knock-out or knock-down mutants (loss of function mutants) and methods according to the invention as described below relate to making such double mutants.

[0059] The plants of the invention are mutant plants which have been genetically modified and are not naturally occurring varieties. Thus, the plants have been generated using genetic engineering methods, for example mutagenesis, gene targeting, gene silencing or genome editing as detailed below. Thus, the various aspects of the invention can involve recombinant DNA technology. The plant may be a transgenic plant in some embodiments, for example a plant which comprises a transgene to silence gene expression of SOD7 and/or NGAL3. In other embodiments, the plant does not carry a transgene, but is a mutant plant wherein the endogenous nucleic acid sequence encoding a NGAL2 and/or NGAL3 polypeptide or the endogenous SOD7 and/or NGAL3 promoter sequence has been manipulated to either reduce or abolish expression of a nucleic acid sequence encoding a NGAL2 and/or NGAL3 polypeptide or reduce or abolish the activity of a NGAL2 and/or NGAL3 polypeptide. The plants of the various aspects of the invention do not relate to natural variants which have not been manipulated by genetic engineering methods.

[0060] In one aspect, the invention relates to a plant generated by genetic engineering methods wherein the expression of a nucleic acid sequence encoding a NGAL2 and/or NGAL3 polypeptide and/or the activity of a NGAL2 and/or NGAL3 polypeptide is reduced or abolished relative to a control plant. In one embodiment, expression of a nucleic acid sequence encoding a NGAL2 polypeptide or the activity of a NGAL2 polypeptide is reduced or abolished. In another embodiment, expression of a nucleic acid sequence encoding a NGAL3 polypeptide or the activity of a NGAL3 polypeptide is reduced or abolished. In a preferred embodiment the presence of function of both proteins is affected, in other words, the plant is characterised in that expression of a nucleic acid sequence encoding a NGAL2 polypeptide or the activity of a NGAL2 polypeptide is reduced or abolished and also expression of a nucleic acid sequence encoding a NGAL3 polypeptide or the activity of a NGAL3 polypeptide is reduced or abolished in said plant.

[0061] For example, said plant can have reduced or abolished expression of a nucleic acid sequence encoding a NGAL2 polypeptide and reduced or abolished expression of a nucleic acid sequence encoding a NGAL3 polypeptide. In another embodiment, said plant can have reduced or abolished activity of a NGAL2 polypeptide and reduced or abolished activity of a NGAL3 polypeptide. In another embodiment, said plant can have reduced or abolished expression of a nucleic acid sequence encoding a NGAL2 polypeptide and reduced or abolished activity of a NGAL3 polypeptide. In another embodiment, said plant can have reduced or abolished expression of a nucleic acid sequence encoding a NGAL3 polypeptide and reduced or abolished activity of a NGAL2 polypeptide.

[0062] A NGAL2 or NGAL3 polypeptide as described in the various aspects of the invention has a characteristic domain structure as explained below.

[0063] A NGAL2 OR NGLA3 polypeptide as described in the various aspects of the invention comprises a B3 DNA binding domain which has the structure shown in FIG. 12.

[0064] In one embodiment, the domain is: SNNNNNNGGSGDDVACHFQRFDLHRLFIGWRGE (SEQ ID NO:6) or a domain with at least 80%, at least 95% or at least 95% sequence identity thereto.

[0065] A NGAL2 OR NGAL3 polypeptide as described in the various aspects of the invention also comprises a transcriptional repression motif shown in FIG. 12.

[0066] In one embodiment, the domain is: VRLFGVNLE (SEQ ID NO:7) or a domain with at least 95% sequence identity thereto.

[0067] In one embodiment, the NGAL2 protein is AtNGAL2, a functional variant, part or homologue thereof. AtNGAL2 is encoded by AtSOD7. The term AtSOD7 refers to the wild type AtSOD7 nucleic acid sequence comprising or consisting of SEQ ID NO. 1 (CDNA) or SEQ ID NO 2 (genomic DNA). The protein encoded by AtSOD7 is termed AtNGAL2 SEQ ID NO.3. In one embodiment, said functional homologue is not AtNGAL3.

[0068] In one embodiment, the NGAL3 protein is AtNGAL3, a functional variant, part or homologue thereof. The term AtNGAL3 refers to the wild type AtNGAL3 nucleic acid sequence comprising or consisting of SEQ ID NO. 4. The protein encoded by AtNGAL3 is termed AtNGAL3 SEQ ID NO.5.

[0069] The term "functional" refers to the biological function of the NGAL2 or NGAL3, that is their function in controlling organ size, in particular seed size. The terms "functional variant" or "functional part" as used herein, for example with reference to SEQ ID NOs: 1, 2 or 3, or SEQ ID NOs: 4 or 5 refers to a variant gene or polypeptide sequence or part of the gene or polypeptide sequence which retains the biological function of the full non-variant SOD7/NGAL2 or NGAL2/NGAL3 sequence, that is regulation of seed size. Such sequences complement the Atsod7-1D mutant or Atngal3 mutant respectively.

[0070] Thus, it is understood, as those skilled in the art will appreciate, that the aspects of the invention, encompass not only targeting a AtSOD7 and/or AtNGAL3 nucleic acid, for example a nucleic acid sequence comprising or consisting of SEQ ID NO: 1 or SEQ ID NO: 2, or SEQ ID NO: 4 respectively or a polypeptide comprising or consisting of SEQ ID NO: 3, or SEQ ID NO: 5, or a promoter of a AtSOD7 and/or AtNGAL3 nucleic acid. The aspects of the invention encompass also functional variants of AtNGAL2 or AtNGAL3 that do not affect the biological activity and function of the resulting protein. Alterations in a nucleic acid sequence which result in the production of a different amino acid at a given site that do however not affect the functional properties of the encoded polypeptide, are well known in the art. For example, a codon for the amino acid alanine, a hydrophobic amino acid, may be substituted by a codon encoding another less hydrophobic residue, such as glycine, or a more hydrophobic residue, such as valine, leucine, or isoleucine. Similarly, changes which result in substitution of one negatively charged residue for another, such as aspartic acid for glutamic acid, or one positively charged residue for another, such as lysine for arginine, can also produce a functionally equivalent product. Each of the proposed modifications is well within the routine skill in the art, as is determination of retention of biological activity of the encoded products. Also encompassed is a variant that is substantially identical, i.e. has only some sequence variations, for example in non-conserved residues, to the wild type sequences as shown herein and is biologically active.

[0071] Generally, variants of a particular SOD7/NGAL3 nucleotide sequence or NGAL2/NGAL3 polypeptide as described herein will have at least about 60%, preferably at least about 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 92%, 94%, 95%, 96%, 97%, 98% or 99% or more sequence identity to that particular non-variant nucleotide sequence, as determined by sequence alignment programs described elsewhere herein.

[0072] Furthermore, the various the aspects of the invention encompass not only a AtSOD7 and/or AtNGAL3 nucleic acid, for example a nucleic acid sequence comprising or consisting of

[0073] 1 or SEQ ID NO: 2, or SEQ ID NO: 4 respectively or a polypeptide comprising or consisting of SEQ ID NO: 3, or SEQ ID NO: 5, or their functional variants but also homologues of AtSOD7 and/or AtNGAL3 in Arabidopsis or other plants. Also within the scope of the invention are functional variants of such homologues as defined above.

[0074] The term homologue as used herein also designates an AtSOD7 and/or AtNGAL3 orthologue from other plant species. A homologue of AtNGAL2 or AtNGAL3 polypeptide respectively has, in increasing order of preference, at least 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99% overall sequence identity to the amino acid represented by SEQ ID NO: 3 or 5 respectively. Preferably, overall sequence identity is at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, most preferably 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99%.

[0075] In another embodiment, the homologue of a AtSOD7 or AtNGAL3 nucleic acid sequence respectively has, in increasing order of preference, at least 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99% overall sequence identity to the nucleic acid represented by SEQ ID NO: 1 or 2 or 4 respectively.

[0076] Preferably, overall sequence identity is at least 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, most preferably 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or at least 99%. The overall sequence identity is determined using a global alignment algorithm known in the art, such as the Needleman Wunsch algorithm in the program GAP (GCG Wisconsin Package, Accelrys).

[0077] In a preferred embodiment, the NGAL2 or NGAL3 homologue is from a plant that is not Arabidopsis.

[0078] In one embodiment, an AtNGAL2 or a homologue thereof or AtNGAL3 or a homologue thereof comprises a B3 domain having the sequence as defined above

[0079] In one embodiment, an AtNGAL2 or a homologue thereof or AtNGAL3 or a homologue thereof comprises a transcriptional repression motif having the sequence as defined above

[0080] Examples of homologues are shown in FIG. 13 and in SEQ ID NO: 49-145. In certain embodiments, if a plant has more than one AtNGAL2 and/or AtNGAL3 homologue, then all homologues are knocked out or knocked down. Suitable homologues can be identified by sequence comparisons and identifications of conserved domains. There are predictors in the art that can be used to identify such sequences. The function of the homologue can be identified as described herein and a skilled person would thus be able to confirm the function, for example when overexpressed in a plant or knocked out in a plant or when expressed in a plant or by expressing the homologous nucleic acid sequence in an Arabidopsis gain of function mutant.

[0081] Thus, the nucleotide sequences of the invention and described herein can also be used to isolate corresponding sequences from other organisms, particularly other plants, for example crop plants. In this manner, methods such as PCR, hybridization, and the like can be used to identify such sequences based on their sequence homology to the sequences described herein. Topology of the sequences and the characteristic domains structure can also be considered when identifying and isolating homologues. Sequences may be isolated based on their sequence identity to the entire sequence or to fragments thereof. In hybridization techniques, all or part of a known nucleotide sequence is used as a probe that selectively hybridizes to other corresponding nucleotide sequences present in a population of cloned genomic DNA fragments or cDNA fragments (i.e., genomic or cDNA libraries) from a chosen plant. The hybridization probes may be genomic DNA fragments, cDNA fragments, RNA fragments, or other oligonucleotides, and may be labelled with a detectable group, or any other detectable marker. Thus, for example, probes for hybridization can be made by labelling synthetic oligonucleotides based on the ABA-associated sequences of the invention. Methods for preparation of probes for hybridization and for construction of cDNA and genomic libraries are generally known in the art and are disclosed in Sambrook, et al., (1989) Molecular Cloning: A Library Manual (2d ed., Cold Spring Harbor Laboratory Press, Plainview, N.Y.).

[0082] Hybridization of such sequences may be carried out under stringent conditions. By "stringent conditions" or "stringent hybridization conditions" is intended conditions under which a probe will hybridize to its target sequence to a detectably greater degree than to other sequences (e.g., at least 2-fold over background). Stringent conditions are sequence dependent and will be different in different circumstances. By controlling the stringency of the hybridization and/or washing conditions, target sequences that are 100% complementary to the probe can be identified (homologous probing). Alternatively, stringency conditions can be adjusted to allow some mismatching in sequences so that lower degrees of similarity are detected (heterologous probing). Generally, a probe is less than about 1000 nucleotides in length, preferably less than 500 nucleotides in length.

[0083] Typically, stringent conditions will be those in which the salt concentration is less than about 1.5 M Na ion, typically about 0.01 to 1.0 M Na ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30.degree. C. for short probes (e.g., 10 to 50 nucleotides) and at least about 60.degree. C. for long probes (e.g., greater than 50 nucleotides). Duration of hybridization is generally less than about 24 hours, usually about 4 to 12. Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide.

[0084] According to the invention, preferred homologues of AtSOD7 and AtNGAL3 peptides are selected from crop plants, for example cereal crops. Preferred homologues of AtNGAL2 and AtNGAL3 and their polypeptide sequences are also shown in FIG. 13.

[0085] A plant according to the various aspects of the invention, including the transgenic plants, methods and uses described herein may be a monocot or a dicot plant.

[0086] A dicot plant may be selected from the families including, but not limited to Asteraceae, Brassicaceae (e.g. Brassica napus), Chenopodiaceae, Cucurbitaceae, Leguminosae (Caesalpiniaceae, Aesalpiniaceae Mimosaceae, Papilionaceae or Fabaceae), Malvaceae, Rosaceae or Solanaceae. For example, the plant may be selected from lettuce, sunflower, Arabidopsis, broccoli, spinach, water melon, squash, cabbage, tomato, potato, yam, capsicum, tobacco, cotton, okra, apple, rose, strawberry, alfalfa, bean, soybean, field (fava) bean, pea, lentil, peanut, chickpea, apricots, pears, peach, grape vine, bell pepper, chilli or citrus species.

[0087] A monocot plant may, for example, be selected from the families Arecaceae, Amaryllidaceae or Poaceae. For example, the plant may be a cereal crop, such as maize, wheat, rice, barley, oat, sorghum, rye, millet, buckwheat, or a grass crop such as Lolium species or Festuca species, or a crop such as sugar cane, onion, leek, yam or banana.

[0088] Also included are biofuel and bioenergy crops such as rape/canola, sugar cane, sweet sorghum, Panicum virgatum (switchgrass), linseed, lupin and willow, poplar, poplar hybrids, Miscanthus or gymnosperms, such as loblolly pine. Also included are crops for silage (maize), grazing or fodder (grasses, clover, sanfoin, alfalfa), fibres (e.g. cotton, flax), building materials (e.g. pine, oak), pulping (e.g. poplar), feeder stocks for the chemical industry (e.g. high erucic acid oil seed rape, linseed) and for amenity purposes (e.g. turf grasses for golf courses), ornamentals for public and private gardens (e.g. snapdragon, petunia, roses, geranium, Nicotiana sp.) and plants and cut flowers for the home (African violets, Begonias, chrysanthemums, geraniums, Coleus spider plants, Dracaena, rubber plant).

[0089] Preferably, the plant is a crop plant. By crop plant is meant any plant which is grown on a commercial scale for human or animal consumption or use. In a preferred embodiment, the plant is a cereal.

[0090] Most preferred plants are maize, rice, wheat, oilseed rape/canola, sorghum, soybean, sunflower, alfalfa, potato, tomato, tobacco, grape, barley, pea, bean, field bean, lettuce, cotton, sugar cane, sugar beet, broccoli or other vegetable brassicas or poplar.

[0091] The term "plant" as used herein encompasses whole plants, ancestors and progeny of the plants and plant parts, including seeds, fruit, shoots, stems, leaves, roots (including tubers), flowers, and tissues and organs, wherein each of the aforementioned comprise the gene/nucleic acid of interest. The term "plant" also encompasses plant cells, suspension cultures, callus tissue, embryos, meristematic regions, gametophytes, sporophytes, pollen and microspores, again wherein each of the aforementioned comprises the gene/nucleic acid of interest.

[0092] According to the various aspects of the invention, including the plants and methods of the invention, abolishing, inactivating, repressing, reducing or down-regulating the activity of a NGAL2 and/or NGAL3 polypeptide can be achieved through different means. Such means that are within the scope of the various aspects of the invention are methods for abolishing or reducing translation or transcription of the SOD7 and/or NGAL3 gene, destabilizing SOD7 and/or NGAL3 transcript stability, destabilizing NGAL2 and/or NGAL3 polypeptide stability or abolishing or reducing the activation or activity of the NGAL2 and/or NGAL3 or polypeptide. Thus, in one embodiment, endogenous SOD7 and/or NGAL3 gene or its promoter carry a functional mutation so that no full length transcript is made. In another embodiment, the SOD7 and/or NGAL3 gene is silenced in said plant using gene silencing techniques. In another embodiment, the SOD7 and/or NGAL3 nucleic acid sequence has been altered to introduce a mutation which results in a NGAL2/NGAL3 protein with reduced or abolished activity. These embodiments and the techniques used are described in more detail below.

[0093] In another aspect, the invention relates to a method for altering a plant phenotype comprising reducing or abolishing the expression of a nucleic acid sequence encoding a NGAL2 and/or NGAL3 polypeptide and/or reducing or abolishing the activity of a NGAL2 and/or NGAL3 polypeptide relative to a control plant.

[0094] In another aspect, the invention relates to a method for making a plant with an altered phenotype comprising reducing or abolishing the expression of a nucleic acid sequence encoding a NGAL2 and/or NGAL3 polypeptide and/or reducing or abolishing the activity of a NGAL2 and/or NGAL3 polypeptide relative to a control plant.

[0095] As previously described, such methods above use genetic engineering methods.

[0096] In this aspect, a wild type plant may be targeted to simultaneously knock out or down both SOD7 and NGAL3 function. Alternatively, the method may comprise the following steps

[0097] a) Knocking out or down SOD7 function in a first plant;

[0098] b) knocking out or down NGAL3 function in a second plant and

[0099] c) crossing plants regenerated from said first plant with plants regenerated from said second plant.

[0100] In one embodiment of these methods, expression of a nucleic acid sequence encoding a NGAL2 polypeptide or the activity of a NGAL2 polypeptide is reduced or abolished. In another embodiment, expression of a nucleic acid sequence encoding a NGAL3 polypeptide or the activity of a NGAL3 polypeptide is reduced or abolished. In a preferred embodiment, the method comprises reducing or abolishing expression of a nucleic acid sequence encoding a NGAL2 polypeptide or the activity of a NGAL2 polypeptide and reducing or abolishing expression of a nucleic acid sequence encoding a NGAL3 polypeptide or the activity of a NGAL3 polypeptide to create a double loss of function mutant.

[0101] For example, the method comprises reducing or abolishing expression of a nucleic acid sequence encoding a NGAL2 polypeptide and reducing or abolishing expression of a nucleic acid sequence encoding a NGAL3 polypeptide. In another embodiment, the method comprises reducing or abolishing activity of a NGAL2 polypeptide and reducing or abolishing activity of a NGAL3 polypeptide. In another embodiment, the method comprises reducing or abolishing expression of a nucleic acid sequence encoding a NGAL2 polypeptide and reducing or abolishing activity of a NGAL3 polypeptide. In another embodiment the method comprises reducing or abolishing expression of a nucleic acid sequence encoding a NGAL3 polypeptide or reducing or abolishing activity of a NGAL2 polypeptide.

[0102] According to these methods, the phenotype is preferably selected from increased organ size, for example increased seed size or increased seed weight. Increased seed size leads to an increase in yield and the methods of the invention also increased yield.

[0103] The term "yield" in general means a measurable produce of economic value, typically related to a specified crop, to an area, and to a period of time. Individual plant parts directly contribute to yield based on their number, size and/or weight, or the actual yield is the yield per square meter for a crop and year, which is determined by dividing total production (includes both harvested and appraised production) by planted square meters. The term "yield" as described herein relates to yield-related traits and may relate to vegetative biomass (root and/or shoot biomass), to reproductive organs, and/or to propagules (such as seeds) of that plant. Thus, according to the invention, the term yield refers to organ size, in particular seed size and can be measured by assessing seed size or seed weight or cotyledon size.

[0104] The terms "increase", "improve" or "enhance" are interchangeable. Yield or seed size for example is increased by at least a 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% or 10%, preferably at least 15% or 20%, more preferably 25%, 30%, 35%, 40% or 50% or more in comparison to a control plant.

[0105] A control plant as used herein according to all of the aspects of the invention is a plant which has not been modified according to the methods of the invention. Accordingly, the control plant has not been genetically modified to alter either expression of a nucleic acid encoding a NGAL2 or NGAL3 polypeptide or to alter the activity of a NGAL2 or NGAL3 polypeptide as described herein. In one embodiment, the control plant is a wild type plant that has not been genetically altered. In another embodiment, the control plant is a transgenic plant that does not have altered expression of a nucleic acid encoding a NGAL2 or NGAL3 polypeptide or altered activity of a NGAL2 or NGAL3 polypeptide, but has been genetically altered in other ways, for example by expressing a desirable transgene to confer certain traits.

[0106] The reduction, decrease, down-regulation or repression of the activity of the NGAL2 and/or NGAL3 polypeptide or corresponding SOD7 and/or NGAL3 nucleic acid sequences according to the aspects of the invention is at least 10%, 20%, 30%, 40% or 50% in comparison to the control plant.

[0107] For example, the plant is a reduction (knock down) or loss of function (knock out) mutant wherein the function of the SOD7 and/or NGAL3 nucleic acid sequence is reduced or lost compared to a wild type control plant. To this end, a mutation is introduced into the SOD7 and/or NGAL3 nucleic acid sequence or the corresponding promoter sequence which disrupts the transcription of the gene leading to a gene product which is not functional or has a reduced function. The mutation may be a deletion, insertion or substitution. The expression of active protein may thus be abolished by mutating the nucleic acid sequences in the plant cell which encode the NGAL2 or NGAL3 polypeptide and regenerating a plant from the mutated cell. The nucleic acids may be mutated by insertion or deletion of one or more nucleotides. Techniques for the inactivation or knockout of target genes are well-known in the art. These techniques include gene target using vectors that target the gene of interest and which allow integration allows for integration of transgene at a specific site. The targeting construct is engineered to recombine with the target gene, which is accomplished by incorporating sequences from the gene itself into the construct. Recombination then occurs in the region of that sequence within the gene, resulting in the insertion of a foreign sequence to disrupt the gene. With its sequence interrupted, the altered gene will be translated into a nonfunctional protein, if it is translated at all. Other techniques include genome editing (targeted genome engineering) as described below. Using either of these techniques, in preferred embodiment, conserved domains which confer function of NGAL2 or NGAL3 respectively are modified.

[0108] A skilled person will know further approaches can be used to generate such mutants. In one embodiment, insertional mutagenesis is used, for example using T-DNA mutagenesis (which inserts pieces of the T-DNA from the Agrobacterium tumefaciens T-Plasmid into DNA causing either loss of gene function or gain of gene function mutations), site-directed nucleases (SDNs) or transposons as mutagens. Insertional mutagenesis is an alternative means of disrupting gene function and is based on the insertion of foreign DNA into the gene of interest (see Krysan et al, The Plant Cell, Vol. 11, 2283-2290, December 1999).

[0109] In one embodiment, as discussed in the examples, T-DNA may be used as an insertional mutagen which disrupts SOD7 and/or NGAL3 gene expression. T-DNA not only disrupts the expression of the gene into which it is inserted, but also acts as a marker for subsequent identification of the mutation. Since the sequence of the inserted element is known, the gene in which the insertion has occurred can be recovered, using various cloning or PCR-based strategies. The insertion of a piece of T-DNA on the order of 5 to 25 kb in length generally produces a disruption of gene function. If a large enough population of T-DNA transformed lines is generated, there are reasonably good chances of finding a transgenic plant carrying a T-DNA insert within any gene of interest. Transformation of spores with T-DNA is achieved by an Agrobacterium-mediated method which involves exposing plant cells and tissues to a suspension of Agrobacterium cells.

[0110] The details of this method are well known to a skilled person. In short, plant transformation by Agrobacterium results in the integration into the nuclear genome of a sequence called T-DNA, which is carried on a bacterial plasmid. The use of T-DNA transformation leads to stable single insertions. Further mutant analysis of the resultant transformed lines is straightforward and each individual insertion line can be rapidly characterized by direct sequencing and analysis of DNA flanking the insertion. Gene expression in the mutant is compared to expression of the SOD7 and/or NGAL3 nucleic acid sequence in a wild type plant and phenotypic analysis is also carried out. Other techniques for insertional mutagenesis include the use of transposons.

[0111] In another embodiment, mutagenesis is physical mutagenesis, such as application of ultraviolet radiation, X-rays, gamma rays, fast or thermal neutrons or protons. The targeted population can then be screened to identify a SOD7 or NGAL3 loss of function mutant.

[0112] In another embodiment of the various aspects of the invention, the plant is a mutant plant derived from a plant population mutagenised with a mutagen. The mutagen may be fast neutron irradiation or a chemical mutagen, for example selected from the following non-limiting list: ethyl methanesulfonate (EMS), methylmethane sulfonate (MMS), N-ethyl-N-nitrosurea (ENU), triethylmelamine (1'EM), N-methyl-N-nitrosourea (MNU), procarbazine, chlorambucil, cyclophosphamide, diethyl sulfate, acrylamide monomer, melphalan, nitrogen mustard, vincristine, dimethylnitosamine, N-methyl-N'-nitro-Nitrosoguanidine (MNNG), nitrosoguanidine, 2-aminopurine, 7,12 dimethyl-benz(a)anthracene (DMBA), ethylene oxide, hexamethylphosphoramide, bisulfan, diepoxyalkanes (diepoxyoctane (DEO), diepoxybutane (BEB), and the like), 2-methoxy-6-chloro-9 [3-(ethyl-2-chloroethyl)aminopropylamino]acridine dihydrochloride (ICR-170) or formaldehyde.

[0113] In one embodiment, the method used to create and analyse mutations is targeting induced local lesions in genomes (TLLING), reviewed in Henikoff et al, 2004. In this method, seeds are mutagenised with a chemical mutagen, for example EMS. The resulting M1 plants are self-fertilised and the M2 generation of individuals is used to prepare DNA samples for mutational screening. DNA samples are pooled and arrayed on microtiter plates and subjected to gene specific PCR. The PCR amplification products may be screened for mutations in the SOD7 and/or NGAL3 target gene using any method that identifies heteroduplexes between wild type and mutant genes. For example, but not limited to, denaturing high pressure liquid chromatography (dHPLC), constant denaturant capillary electrophoresis (CDCE), temperature gradient capillary electrophoresis (TGCE), or by fragmentation using chemical cleavage. Preferably the PCR amplification products are incubated with an endonuclease that preferentially cleaves mismatches in heteroduplexes between wild type and mutant sequences. Cleavage products are electrophoresed using an automated sequencing gel apparatus, and gel images are analyzed with the aid of a standard commercial image-processing program. Any primer specific to the SOD7 or NGAL3 nucleic acid sequence may be utilized to amplify the SOD7 or NGAL3 nucleic acid sequence within the pooled DNA sample. Preferably, the primer is designed to amplify the regions of the SOD7 and/or NGAL3 gene where useful mutations are most likely to arise, specifically in the areas of the SOD7 and/or NGAL3 gene that are highly conserved and/or confer activity as explained elsewhere. To facilitate detection of PCR products on a gel, the PCR primer may be labelled using any conventional labelling method.

[0114] Rapid high-throughput screening procedures thus allow the analysis of amplification products for identifying a mutation conferring the reduction or inactivation of the expression of the SOD7 and/or NGAL3 gene as compared to a corresponding non-mutagenised wild type plant. Once a mutation is identified in a gene of interest, the seeds of the M2 plant carrying that mutation are grown into adult M3 plants and screened for the phenotypic characteristics associated with the target gene SOD7 or NGAL3. Loss of function or reduced function mutants with increased seed size compared to a control can thus be identified.

[0115] Plants obtained or obtainable by such method which carry a functional mutation in the endogenous SOD7 and/or NGAL3 locus are also within the scope of the invention

[0116] In another embodiment, RNA-mediated gene suppression or RNA silencing may be used to achieve silencing of the SOD7 and/or NGAL3 nucleic acid sequence. "Gene silencing" is a term generally used to refer to suppression of expression of a gene via sequence-specific interactions that are mediated by RNA molecules. The degree of reduction may be so as to totally abolish production of the encoded gene product, but more usually the abolition of expression is partial, with some degree of expression remaining. The term should not therefore be taken to require complete "silencing" of expression.

[0117] Transgenes may be used to suppress endogenous plant genes. This was discovered originally when chalcone synthase transgenes in petunia caused suppression of the endogenous chalcone synthase genes and indicated by easily visible pigmentation changes. Subsequently it has been described how many, if not all plant genes can be "silenced" by transgenes. Gene silencing requires sequence similarity between the transgene and the gene that becomes silenced. This sequence homology may involve promoter regions or coding regions of the silenced target gene. When coding regions are involved, the transgene able to cause gene silencing may have been constructed with a promoter that would transcribe either the sense or the antisense orientation of the coding sequence RNA. It is likely that the various examples of gene silencing involve different mechanisms that are not well understood. In different examples there may be transcriptional or post-transcriptional gene silencing and both may be used according to the methods of the invention.

[0118] The mechanisms of gene silencing and their application in genetic engineering, which were first discovered in plants in the early 1990s and then shown in Caenorhabditis elegans are extensively described in the literature.

[0119] RNA-mediated gene suppression or RNA silencing according to the methods of the invention includes co-suppression wherein over-expression of the target sense RNA or mRNA, that is the SOD7 and/or NGAL3 sense RNA or mRNA, leads to a reduction in the level of expression of the genes concerned. RNAs of the transgene and homologous endogenous gene are coordinately suppressed. Other techniques used in the methods of the invention include antisense RNA to reduce transcript levels of the endogenous target gene in a plant. In this method, RNA silencing does not affect the transcription of a gene locus, but only causes sequence-specific degradation of target mRNAs. An "antisense" nucleic acid sequence comprises a nucleotide sequence that is complementary to a "sense" nucleic acid sequence encoding a NGAL2 and/or NGAL3 protein, or a part of the protein, i.e. complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA transcript sequence. The antisense nucleic acid sequence is preferably complementary to the endogenous SOD7 and/or NGAL3 gene to be silenced. The complementarity may be located in the "coding region" and/or in the "non-coding region" of a gene. The term "coding region" refers to a region of the nucleotide sequence comprising codons that are translated into amino acid residues. The term "non-coding region" refers to 5' and 3' sequences that flank the coding region that are transcribed but not translated into amino acids (also referred to as 5' and 3' untranslated regions).

[0120] Antisense nucleic acid sequences can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid sequence may be complementary to the entire SOD7 and/or NGAL3 nucleic acid sequence, but may also be an oligonucleotide that is antisense to only a part of the nucleic acid sequence (including the mRNA 5' and 3' UTR). For example, the antisense oligonucleotide sequence may be complementary to the region surrounding the translation start site of an mRNA transcript encoding a polypeptide. The length of a suitable antisense oligonucleotide sequence is known in the art and may start from about 50, 45, 40, 35, 30, 25, 20, 15 or 10 nucleotides in length or less. An antisense nucleic acid sequence according to the invention may be constructed using chemical synthesis and enzymatic ligation reactions using methods known in the art. For example, an antisense nucleic acid sequence (e.g., an antisense oligonucleotide sequence) may be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acid sequences, e.g., phosphorothioate derivatives and acridine-substituted nucleotides may be used. Examples of modified nucleotides that may be used to generate the antisense nucleic acid sequences are well known in the art. The antisense nucleic acid sequence can be produced biologically using an expression vector into which a nucleic acid sequence has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest). Preferably, production of antisense nucleic acid sequences in plants occurs by means of a stably integrated nucleic acid construct comprising a promoter, an operably linked antisense oligonucleotide, and a terminator.

[0121] The nucleic acid molecules used for silencing in the methods of the invention hybridize with or bind to mRNA transcripts and/or insert into genomic DNA encoding a polypeptide to thereby inhibit expression of the protein, e.g., by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid sequence which binds to DNA duplexes, through specific interactions in the major groove of the double helix. Antisense nucleic acid sequences may be introduced into a plant by transformation or direct injection at a specific tissue site. Alternatively, antisense nucleic acid sequences can be modified to target selected cells and then administered systemically. For example, for systemic administration, antisense nucleic acid sequences can be modified such that they specifically bind to receptors or antigens expressed on a selected cell surface, e.g., by linking the antisense nucleic acid sequence to peptides or antibodies which bind to cell surface receptors or antigens. The antisense nucleic acid sequences can also be delivered to cells using vectors.

[0122] RNA interference (RNAi) is another post-transcriptional gene-silencing phenomenon which may be used according to the methods of the invention. This is induced by double-stranded RNA in which mRNA that is homologous to the dsRNA is specifically degraded. It refers to the process of sequence-specific post-transcriptional gene silencing mediated by short interfering RNAs (siRNA). The process of RNAi begins when the enzyme, DICER, encounters dsRNA and chops it into pieces called small-interfering RNAs (siRNA). This enzyme belongs to the RNase III nuclease family. A complex of proteins gathers up these RNA remains and uses their code as a guide to search out and destroy any RNAs in the cell with a matching sequence, such as target mRNA.

[0123] Artificial and/or natural microRNAs (miRNAs) may be used to knock out gene expression and/or mRNA translation. MicroRNAs (miRNAs) miRNAs are typically single stranded small RNAs typically 19-24 nucleotides long. Most plant miRNAs have perfect or near-perfect complementarity with their target sequences. However, there are natural targets with up to five mismatches. They are processed from longer non-coding RNAs with characteristic fold-back structures by double-strand specific RNases of the Dicer family. Upon processing, they are incorporated in the RNA-induced silencing complex (RISC) by binding to its main component, an Argonaute protein. miRNAs serve as the specificity components of RISC, since they base-pair to target nucleic acids, mostly mRNAs, in the cytoplasm. Subsequent regulatory events include target mRNA cleavage and destruction and/or translational inhibition. Effects of miRNA overexpression are thus often reflected in decreased mRNA levels of target genes. Artificial microRNA (amiRNA) technology has been applied in Arabidopsis thaliana and other plants to efficiently silence target genes of interest. The design principles for amiRNAs have been generalized and integrated into a Web-based tool (wmd.weigelworld.org).

[0124] Thus, according to the various aspects of the invention a plant may be transformed to introduce a RNAi, shRNA, snRNA, dsRNA, siRNA, miRNA, ta-siRNA, amiRNA or cosuppression molecule that has been designed to target the expression of an SOD7 and/or NGAL3 nucleic acid sequence and selectively decreases or inhibits the expression of the gene or stability of its transcript. Preferably, the RNAi, snRNA, dsRNA, shRNA siRNA, miRNA, amiRNA, to-siRNA or cosuppression molecule used according to the various aspects of the invention comprises a fragment of at least 17 nt, preferably 22 to 26 nt and can be designed on the basis of the information shown in SEQ ID NO: 1. Guidelines for designing effective siRNAs are known to the skilled person. Briefly, a short fragment of the target gene sequence (e.g., 19-40 nucleotides in length) is chosen as the target sequence of the siRNA of the invention. The short fragment of target gene sequence is a fragment of the target gene mRNA. In preferred embodiments, the criteria for choosing a sequence fragment from the target gene mRNA to be a candidate siRNA molecule include 1) a sequence from the target gene mRNA that is at least 50-100 nucleotides from the 5' or 3' end of the native mRNA molecule, 2) a sequence from the target gene mRNA that has a G/C content of between 30% and 70%, most preferably around 50%, 3) a sequence from the target gene mRNA that does not contain repetitive sequences (e.g., AAA, CCC, GGG, TTT, AAAA, CCCC, GGGG, TTTT), 4) a sequence from the target gene mRNA that is accessible in the mRNA, 5) a sequence from the target gene mRNA that is unique to the target gene, 6) avoids regions within 75 bases of a start codon. The sequence fragment from the target gene mRNA may meet one or more of the criteria identified above. The selected gene is introduced as a nucleotide sequence in a prediction program that takes into account all the variables described above for the design of optimal oligonucleotides. This program scans any mRNA nucleotide sequence for regions susceptible to be targeted by siRNAs. The output of this analysis is a score of possible siRNA oligonucleotides. The highest scores are used to design double stranded RNA oligonucleotides that are typically made by chemical synthesis. In addition to siRNA which is complementary to the mRNA target region, degenerate siRNA sequences may be used to target homologous regions. siRNAs according to the invention can be synthesized by any method known in the art. RNAs are preferably chemically synthesized using appropriately protected ribonucleoside phosphoramidites and a conventional DNA/RNA synthesizer. Additionally, siRNAs can be obtained from commercial RNA oligonucleotide synthesis suppliers.

[0125] siRNA molecules according to the aspects of the invention may be double stranded. In one embodiment, double stranded siRNA molecules comprise blunt ends. In another embodiment, double stranded siRNA molecules comprise overhanging nucleotides (e.g., 1-5 nucleotide overhangs, preferably 2 nucleotide overhangs). In some embodiments, the siRNA is a short hairpin RNA (shRNA); and the two strands of the siRNA molecule may be connected by a linker region (e.g., a nucleotide linker or a non-nucleotide linker). The siRNAs of the invention may contain one or more modified nucleotides and/or non-phosphodiester linkages. Chemical modifications well known in the art are capable of increasing stability, availability, and/or cell uptake of the siRNA. The skilled person will be aware of other types of chemical modification which may be incorporated into RNA molecules.

[0126] In one embodiment, recombinant DNA constructs as described in U.S. Pat. No. 6,635,805, incorporated herein by reference, may be used.

[0127] The silencing RNA molecule is introduced into the plant using conventional methods, for example a vector and Agrobacterium-mediated transformation. Stably transformed plants are generated and expression of the SOD7 and/or NGAL3 gene compared to a wild type control plant is analysed.

[0128] Silencing of the SOD7 and/or NGAL3 nucleic acid sequence may also be achieved using virus-induced gene silencing.

[0129] Thus, in one embodiment of the invention, the plant expresses a nucleic acid construct comprising a RNAi, shRNA snRNA, dsRNA, siRNA, miRNA, ta-siRNA, amiRNA or co-suppression molecule that targets the SOD7 or NGAL3 nucleic acid sequence as described herein and reduces expression of the endogenous SOD7 or NGAL3 nucleic acid sequence. A gene is targeted when, for example, the RNAi, snRNA, dsRNA, siRNA, shRNA miRNA, ta-siRNA, amiRNA or cosuppression molecule selectively decreases or inhibits the expression of the gene compared to a control plant. Alternatively, a RNAi, snRNA, dsRNA, siRNA, miRNA, ta-siRNA, amiRNA or cosuppression molecule targets A SOD7 or NGAL3 nucleic acid sequence when the RNAi, shRNA snRNA, dsRNA, siRNA, miRNA, ta-siRNA, amiRNA or cosuppression molecule hybridises under stringent conditions to the gene transcript.

[0130] Gene silencing may also occur if there is a mutation on an endogenous gene and/or a mutation on an isolated gene/nucleic acid subsequently introduced into a plant. The reduction or substantial elimination may be caused by a non-functional polypeptide. For example, the polypeptide may bind to various interacting proteins; one or more mutation(s) and/or truncation(s) may therefore provide for a polypeptide that is still able to bind interacting proteins (such as receptor proteins) but that cannot exhibit its normal function (such as signalling ligand).

[0131] A further approach to gene silencing is by targeting nucleic acid sequences complementary to the regulatory region of the gene (e.g., the promoter and/or enhancers) to form triple helical structures that prevent transcription of the gene in target cells. Other methods, such as the use of antibodies directed to an endogenous polypeptide for inhibiting its function in planta, or interference in the signalling pathway in which a polypeptide is involved, will be well known to the skilled man. In particular, it can be envisaged that manmade molecules may be useful for inhibiting the biological function of a target polypeptide, or for interfering with the signalling pathway in which the target polypeptide is involved.

[0132] In one embodiment, the suppressor nucleic acids may be anti-sense suppressors of expression of the NGAL2 or NGAL3 polypeptides. In using anti-sense sequences to down-regulate gene expression, a nucleotide sequence is placed under the control of a promoter in a "reverse orientation" such that transcription yields RNA which is complementary to normal mRNA transcribed from the "sense" strand of the target gene.

[0133] An anti-sense suppressor nucleic acid may comprise an anti-sense sequence of at least 10 nucleotides from the target nucleotide sequence. It may be preferable that there is complete sequence identity in the sequence used for down-regulation of expression of a target sequence, and the target sequence, although total complementarity or similarity of sequence is not essential. One or more nucleotides may differ in the sequence used from the target gene. Thus, a sequence employed in a down-regulation of gene expression in accordance with the present invention may be a wild-type sequence (e.g. gene) selected from those available, or a variant of such a sequence.

[0134] The sequence need not include an open reading frame or specify an RNA that would be translatable. It may be preferred for there to be sufficient homology for the respective anti-sense and sense RNA molecules to hybridise. There may be down regulation of gene expression even where there is about 5%, 10%, 15% or 20% or more mismatch between the sequence used and the target gene. Effectively, the homology should be sufficient for the down-regulation of gene expression to take place.

[0135] Suppressor nucleic acids may be operably linked to tissue-specific or inducible promoters. For example, integument and seed specific promoters can be used to specifically down-regulate a SOD7 or NGAL3 nucleic acids in developing ovules and seeds to increase final seed size.

[0136] Nucleic acid which suppresses expression of a NGAL2 or NGAL3 polypeptide as described herein may be operably linked to a heterologous regulatory sequence, such as a promoter, for example a constitutive, inducible, tissue-specific or developmental specific promoter. The construct or vector may be transformed into plant cells and expressed as described herein. Plant cells comprising such vectors are also within the scope of the invention.

[0137] In another aspect, the invention relates to a silencing construct to silence expression of NGAL2 or NGAL3 obtainable or obtained by a method as described herein and to a plant cell comprising such construct. Accordingly, the invention also relates to the use of a nucleic acid sequence comprising or consisting of SEQ ID NO: 1, 2 or 3 or a part thereof or a homologue of SEQ ID NO: 1, 2 or 3 or a part thereof in silencing expression of NGAL2 or NGAL3. Host cells transformed with such construct are also within the scope of the invention.

[0138] Recently, genome editing techniques have emerged as alternative methods to conventional mutagenesis methods (such as physical and chemical mutagenesis) or methods using the expression of transgenes in plants to produce mutant plants with improved phenotypes that are important in agriculture. These techniques employ sequence-specific nucleases (SSNs) including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the RNA-guided nuclease Cas9 (CRISPR/Cas9), which generate targeted DNA double-strand breaks (DSBs), which are then repaired mainly by either error-prone non-homologous end joining (NHEJ) or high-fidelity homologous recombination (HR). The SSNs have been used to create targeted knockout plants in various species ranging from the model plants, Arabidopsis and tobacco, to important crops, such as barley, soybean, rice and maize. Heritable gene modification has been demonstrated in Arabidopsis and rice using the CRISPR/Cas9 system and TALENs.

[0139] Targeted genome modification or targeted genome editing is a genome engineering technique that uses targeted DNA double-strand breaks (DSBs) to stimulate genome editing through homologous recombination (HR)-mediated recombination events. To achieve effective genome editing via introduction of site-specific DNA DSBs, four major classes of customizable DNA binding proteins can be used: meganucleases derived from microbial mobile genetic elements, ZF nucleases based on eukaryotic transcription factors, transcription activator-like effectors (TALEs) from Xanthomonas bacteria, and the RNA-guided DNA endonuclease Cas9 from the type II bacterial adaptive immune system CRISPR (clustered regularly interspaced short palindromic repeats). Meganuclease, ZF, and TALE proteins all recognize specific DNA sequences through protein-DNA interactions. Although meganucleases integrate its nuclease and DNA-binding domains, ZF and TALE proteins consist of individual modules targeting 3 or 1 nucleotides (nt) of DNA, respectively. ZFs and TALEs can be assembled in desired combinations and attached to the nuclease domain of Fokl to direct nucleolytic activity toward specific genomic loci.

[0140] Upon delivery into host cells via the bacterial type III secretion system, TAL effectors enter the nucleus, bind to effector-specific sequences in host gene promoters and activate transcription. Their targeting specificity is determined by a central domain of tandem, 33-35 amino acid repeats. This is followed by a single truncated repeat of 20 amino acids. The majority of naturally occurring TAL effectors examined have between 12 and 27 full repeats.

[0141] These repeats only differ from each other by two adjacent amino acids, their repeat-variable di-residue (RVD). The RVD that determines which single nucleotide the TAL effector will recognize: one RVD corresponds to one nucleotide, with the four most common RVDs each preferentially associating with one of the four bases. Naturally occurring recognition sites are uniformly preceded by a T that is required for TAL effector activity. TAL effectors can be fused to the catalytic domain of the Fokl nuclease to create a TAL effector nuclease (TALEN) which makes targeted DNA double-strand breaks (DSBs) in vivo for genome editing. The use of this technology in genome editing is well described in the art, for example in U.S. Pat. Nos. 8,440,431, 8,440,432 and 8,450,471. Reference 30 describes a set of customized plasmids that can be used with the Golden Gate cloning method to assemble multiple DNA fragments. As described therein, the Golden Gate method uses Type IIS restriction endonucleases, which cleave outside their recognition sites to create unique 4 bp overhangs. Cloning is expedited by digesting and ligating in the same reaction mixture because correct assembly eliminates the enzyme recognition site. Assembly of a custom TALEN or TAL effector construct and involves two steps: (i) assembly of repeat modules into intermediary arrays of 1-10 repeats and (ii) joining of the intermediary arrays into a backbone to make the final construct.

[0142] Another genome editing method that can be used according to the various aspects of the invention is CRISPR. The use of this technology in genome editing is well described in the art, for example in U.S. Pat. No. 8,697,359 and references cited herein. In short, CRISPR is a microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage (sgRNA). Three types (I-III) of CRISPR systems have been identified across a wide range of bacterial hosts. One key feature of each CRISPR locus is the presence of an array of repetitive sequences (direct repeats) interspaced by short stretches of non-repetitive sequences (spacers). The non-coding CRISPR array is transcribed and cleaved within direct repeats into short crRNAs containing individual spacer sequences, which direct Cas nucleases to the target site (protospacer). The Type II CRISPR is one of the most well characterized systems and carries out targeted DNA double-strand break in four sequential steps. First, two non-coding RNA, the pre-crRNA array and tracrRNA, are transcribed from the CRISPR locus. Second, tracrRNA hybridizes to the repeat regions of the pre-crRNA and mediates the processing of pre-crRNA into mature crRNAs containing individual spacer sequences. Third, the mature crRNA:tracrRNA complex directs Cas9 to the target DNA via Watson-Crick base-pairing between the spacer on the crRNA and the protospacer on the target DNA next to the protospacer adjacent motif (PAM), an additional requirement for target recognition. Finally, Cas9 mediates cleavage of target DNA to create a double-stranded break within the protospacer.

[0143] Cas9 is thus the hallmark protein of the type II CRISPR-Cas system, and a large monomeric DNA nuclease guided to a DNA target sequence adjacent to the PAM (protospacer adjacent motif) sequence motif by a complex of two noncoding RNAs: CRIPSR RNA (crRNA) and trans-activating crRNA (tracrRNA). The Cas9 protein contains two nuclease domains homologous to RuvC and HNH nucleases. The HNH nuclease domain cleaves the complementary DNA strand whereas the RuvC-like domain cleaves the non-complementary strand and, as a result, a blunt cut is introduced in the target DNA. Heterologous expression of Cas9 together with an sgRNA can introduce site-specific double strand breaks (DSBs) into genomic DNA of live cells from various organisms. For applications in eukaryotic organisms, codon optimized versions of Cas9, which is originally from the bacterium Streptococcus pyogenes, have been used.

[0144] The single guide RNA (sgRNA) is the second component of the CRISPR/Cas system that forms a complex with the Cas9 nuclease. sgRNA is a synthetic RNA chimera created by fusing crRNA with tracrRNA. The sgRNA guide sequence located at its 5' end confers DNA target specificity. Therefore, by modifying the guide sequence, it is possible to create sgRNAs with different target specificities. The canonical length of the guide sequence is 20 bp. In plants, sgRNAs have been expressed using plant RNA polymerase III promoters, such as U6 and U3.

[0145] Using these techniques, it is possible to specifically target conserved domains to abolish the function of the NGAL2 and/or NGAL3 polypeptide.

[0146] For example, the conserved B3 domain or repression motif may be targeted.

[0147] Thus, in another embodiment of the invention directed to a mutant plant, plant cell, plant or a part thereof characterised in that the activity of a NGAL2 polypeptide is altered and said plant expresses a nucleic acid comprising a mutant SEQ ID NO. 1 or 2 and encoding a mutant NGAL2 polypeptide, a functional homologue or variant thereof, for example one which carries a mutation in the B3 or repressor domain.

[0148] Thus, in another embodiment of the invention directed to a mutant plant, plant cell, plant or a part thereof characterised in that the activity of a NGAL3 polypeptide is altered and said plant expresses a nucleic acid comprising a mutant SEQ ID NO. 4 and encoding a mutant NGAL3 polypeptide, a functional homologue or variant thereof which carries a mutation in the B3 or repressor domain.

[0149] In a preferred embodiment, the invention directed to a mutant plant, plant cell, plant or a part thereof characterised in that the activity of a NGAL2 and a NGAL3 polypeptide is altered and said plant expresses a nucleic acid comprising a mutant SEQ ID NO. 1 or 2 and encoding a mutant NGAL2 polypeptide, a functional homologue or variant thereof, for example one which carries a mutation in the B3 or repressor domain and said plant expresses a nucleic acid comprising a mutant SEQ ID NO. 4 and encoding a mutant NGAL3 polypeptide which carries a mutation in the B3 or repressor domain.

[0150] Mutations in the promoter region of SOD7 and/or NGAL3 resulting in a loss of function are also within the scope of the invention.

[0151] Constructs designed using the genome editing technologies to knock out or knock down NGAL2 or NGAL3, for example as shown herein, are also within the scope of the invention as well as host cells comprising these constructs. In one embodiment, the constructs comprise or consist of a sequence selected from SEQ ID NO: 155, 156, 157 or 158. Accordingly, in a further aspect of the invention, there is provided a nucleic acid construct comprising a sequence selected from SEQ ID NO: 155, 156, 157 or 158. In a further aspect of the invention, there is provided a nucleic acid construct comprising at least one CRISPR target sequence, wherein the target sequence is selected from SEQ ID Nos 150, 160, 161, 162 and 163. Preferably, the target sequence comprises at least two CRISPR target sequences, preferably SEQ ID No 159 and 160 or SEQ ID No 161 and 162, or SEQ ID No 161 and 163 or SEQ ID No 159 and 163.

[0152] In another embodiment of the methods of the invention, inactivating, repressing or down-regulating the activity of NGAL2 and/or NGAL3 can be achieved by manipulating the expression of SOD7 and/or NGAL3 inhibitors in a plant, for example transgenic plant. For example, a gene expressing a protein that inhibits the expression of the SOD7 and/or NGAL3 gene or activity of the SOD7 and/or NGAL3 protein can be introduced into a plant and over-expressed. The inhibitor may interact with the regulatory sequences that direct SOD7 and/or NGAL3 gene expression to down-regulate or repress SOD7 and/or NGAL3 gene expression. For example, the inhibitor may be a transcriptional repressor. Alternatively, it may interact and repress transcriptional regulators, for example transcription factors, that positively regulate expression of the SOD7 and/or NGAL3 gene. Alternatively, the inhibitor it may directly interact with the NGAL2 and/or NGAL3 protein to inhibit its activity or interact with modulators of the NGAL2 and/or NGAL3 protein. For example, the activity of the NGAL2 and/or NGAL3 protein may be inactivated, repressed or down-regulated by manipulating post-transcriptional modifications, of the NGAL2 and/or NGAL3 protein resulting in a reduced or lost activity.

[0153] In one embodiment, the methods of the invention comprise comparing the activity of the NGAL2 and/or NGAL3 polypeptide and/or expression of the SOD7 and/or NGAL3 gene with the activity of the NGAL2 and/or NGAL3 polypeptide and/or expression of the SOD7 and/or NGAL3 gene in a control plant.

[0154] In another aspect, the invention relates to a plant obtainable or obtained by a method as described herein.

[0155] In another aspect, the invention relates to an expression cassette comprising an isolated nucleic acid sequence comprising or consisting of a sequence as shown in SEQ ID NO: 1 or 2 a functional part, variant, homologue or orthologue thereof operably linked to a regulatory element. In another aspect, the invention relates to an expression cassette comprising an isolated nucleic acid sequence comprising or consisting of a sequence as shown in SEQ ID NO: 4 or a functional part, variant, homologue or orthologue thereof operably linked to a regulatory element. The regulatory element may be a promoter. The invention also relates to a vector comprising such expression cassette. The invention also relates to a composition comprising the two expression cassettes above.

[0156] In the methods described here, plants can be regenerated from plants transformed or genetically altered as described above and the phenotype, specifically the seed phenotype is analysed by known methods.

[0157] Transformation methods are known in the art. The nucleic acid sequence is introduced into said plant through a process called transformation. The term "introduction" or "transformation" as referred to herein encompasses the transfer of an exogenous polynucleotide into a host cell, irrespective of the method used for transfer. Plant tissue capable of subsequent clonal propagation, whether by organogenesis or embryogenesis, may be transformed with a genetic construct of the present invention and a whole plant regenerated there from. The particular tissue chosen will vary depending on the clonal propagation systems available for, and best suited to, the particular species being transformed. Exemplary tissue targets include leaf disks, pollen, embryos, cotyledons, hypocotyls, megagametophytes, callus tissue, existing meristematic tissue (e.g., apical meristem, axillary buds, and root meristems), and induced meristem tissue (e.g., cotyledon meristem and hypocotyl meristem). The polynucleotide may be transiently or stably introduced into a host cell and may be maintained non-integrated, for example, as a plasmid. Alternatively, it may be integrated into the host genome. The resulting transformed plant cell may then be used to regenerate a transformed plant in a manner known to persons skilled in the art.

[0158] The transfer of foreign genes into the genome of a plant is called transformation. Transformation of plants is now a routine technique in many species. Advantageously, any of several transformation methods may be used to introduce the gene of interest into a suitable ancestor cell. The methods described for the transformation and regeneration of plants from plant tissues or plant cells may be utilized for transient or for stable transformation. Transformation methods include the use of liposomes, electroporation, chemicals that increase free DNA uptake, injection of the DNA directly into the plant, particle gun bombardment, transformation using viruses or pollen and microprojection. Methods may be selected from the calcium/polyethylene glycol method for protoplasts, electroporation of protoplasts, microinjection into plant material, DNA or RNA-coated particle bombardment, infection with (non-integrative) viruses and the like. Transgenic plants, including transgenic crop plants, are preferably produced via Agrobacterium tumefaciens mediated transformation.

[0159] To select transformed plants, the plant material obtained in the transformation is, as a rule, subjected to selective conditions so that transformed plants can be distinguished from untransformed plants. For example, the seeds obtained in the above-described manner can be planted and, after an initial growing period, subjected to a suitable selection by spraying. A further possibility is growing the seeds, if appropriate after sterilization, on agar plates using a suitable selection agent so that only the transformed seeds can grow into plants. Alternatively, the transformed plants are screened for the presence of a selectable marker such as the ones described above. Following DNA transfer and regeneration, putatively transformed plants may also be evaluated, for instance using Southern analysis, for the presence of the gene of interest, copy number and/or genomic organisation. Alternatively or additionally, expression levels of the newly introduced DNA may be monitored using Northern and/or Western analysis, both techniques being well known to persons having ordinary skill in the art.

[0160] The generated transformed plants may be propagated by a variety of means, such as by clonal propagation or classical breeding techniques. For example, a first generation (or T1) transformed plant may be selfed and homozygous second-generation (or T2) transformants selected, and the T2 plants may then further be propagated through classical breeding techniques. The generated transformed organisms may take a variety of forms. For example, they may be chimeras of transformed cells and non-transformed cells; clonal transformants (e.g., all cells transformed to contain the expression cassette); grafts of transformed and untransformed tissues (e.g., in plants, a transformed rootstock grafted to an untransformed scion).

[0161] The various aspects of the invention described herein clearly extend to any plant cell or any plant produced, obtained or obtainable by any of the methods described herein, and to all plant parts and propagules thereof unless otherwise specified. The present invention extends further to encompass the progeny of a primary transformed or transfected cell, tissue, organ or whole plant that has been produced by any of the aforementioned methods, the only requirement being that progeny exhibit the same genotypic and/or phenotypic characteristic(s) as those produced by the parent in the methods according to the invention.

[0162] The invention also extends to harvestable parts of a plant of the invention as described above such as, but not limited to seeds, leaves, fruits, flowers, stems, roots, rhizomes, tubers and bulbs. The invention furthermore relates to products derived, preferably directly derived, from a harvestable part of such a plant, such as dry pellets or powders, oil, fat and fatty acids, starch or proteins. The invention also relates to food products and food supplements comprising the plant of the invention or parts thereof.

[0163] While the foregoing disclosure provides a general description of the subject matter encompassed within the scope of the present invention, including methods, as well as the best mode thereof, of making and using this invention, the following examples are provided to further enable those skilled in the art to practice this invention and to provide a complete written description thereof. However, those skilled in the art will appreciate that the specifics of these examples should not be read as limiting on the invention, the scope of which should be apprehended from the claims and equivalents thereof appended to this disclosure. Various further aspects and embodiments of the present invention will be apparent to those skilled in the art in view of the present disclosure.

[0164] All documents mentioned in this specification are incorporated herein by reference in their entirety, including references to gene and protein accession numbers.

[0165] "and/or" where used herein is to be taken as specific disclosure of each of the multiple specified features or components with or without the other at each combination unless otherwise dictated. For example "A, B and/or C" is to be taken as specific disclosure of each of (i) A, (ii) B, (iii) C, (iv) A and B, (v) B and C or (vi) A and B and C, just as if each is set out individually herein.

[0166] Unless context dictates otherwise, the descriptions and definitions of the features set out above are not limited to any particular aspect or embodiment of the invention and apply equally to all aspects and embodiments which are described.

[0167] The invention is further described in the following non-limiting examples.

EXAMPLES

[0168] Methods

[0169] Plant Materials and Growth Conditions

[0170] Arabidopsis thaliana Columbia (Col-0) was used as wild-type line. The da1-1, sod7-1D, sod7-ko1 and ngal3-ko1 were in the Col-0 background. sod7-1D was identified as a suppressor of da1-1 by using T-DNA activation tagging method. The sod7-ko1 (SM_3_34191) and ngal3-ko1 (SM_3_36641) were identified in AtIDB (atidb.org) and obtained from Arabidopsis Stock Centre NASC collection. T-DNA insertions were confirmed by PCR and sequencing by using the primers described in Table 1. Arabidopsis plants were grown under long-day conditions (16 h light/8 h dark) at 22.degree. C. Activation tagging screening The activation tagging plasmid pJFAT260 was introduced into the da1-1 mutant plants using Agrobacterium tumefaciens strain GV3101 (Fan et al., 2009; Fang et al., 2012), and T1 plants were selected by using the herbicide Basta. Seeds produced from T1 plants were used to isolate modifiers of da1-1.

[0171] Morphological and Cellular Analysis

[0172] To measure seed size, we photographed dry seeds of the wild type and mutants under a Leica microscope (LEICA S8APO) using Leica CCD (DFC420). The projective area of wild-type and mutant seeds was measured by using Image J software. Average seed weight was determined by weighing mature dry seeds in batches of 100 using an electronic analytical balance (METTLER TOLEDO AL104, China). The weights of five sample batches were measured for each seed lot. Fully expanded cotyledons, petals (stage 14) and leaves were scanned to produce digital images for area measurement. To measure cell number and cell size, petals, leaves, ovules and seeds were placed in a drop of clearing solution [30 ml H2O, 80 g Chloral hydrate (Sigma, C8383), 10 ml 100% Glycerol (Sigma, G6279)]. Cleared Samples were imaged under a Leica microscope (LEICA DM2500) with differential interference contrast (DIC) optics and photographed with a SPOT FLEX Cooled CCD Digital Imaging System. Area measurement was made by using Image J software.

[0173] Cloning of the SOD7 Gene

[0174] The flanking sequences of the T-DNA insertion of the sod7-1D mutant were identified by the thermal asymmetric interlaced PCR (TAIL-PCR) according to a previously reported method (Liu et al., 1995). Briefly, TAIL-PCR utilizes three nested specific primers (OJF22, OJF23 and OJF24) within the T-DNA region of the pJFAT260 vector and a shorter arbitrary degenerate primer (AD1). Thus, the relative amplification efficiencies of specific and non-specific products can be thermally controlled. TAIL-PCR products were sequenced using the primer OJF24. The specific primers OJF22, OJF23 and OJF24 and an arbitrary degenerate (AD1) primer are described in Table 1.

[0175] Constructs and Plant Transformation

[0176] The 35S:GFP-SOD7, pSOD7:SOD7-GFP and pSOD7:GUS constructs were made using a PCR-based Gateway system. The coding sequence (CDS) of SOD7 was amplified using the primers SOD7CDS-F and SOD7CDS-R (Table 1). PCR products were cloned into pCR8/TOPO TA cloning vector. The SOD7 CDS was then subcloned into the binary vector pMDC43 with the GFP gene to generate the transformation plasmid 35S:GFP-SOD7. The SOD7 genomic sequence containing 2040-bp promoter sequence and 2104-bp SOD7 gene was amplified using the primers SOD7G-F and SOD7G-R (Table 1). PCR products were cloned into pCR8/TOPO TA cloning vector. The SOD7 genomic sequence was then subcloned into the binary vectors pMDC107 with the GFP gene to generate the transformation plasmid pSOD7:SOD7-GFP. The 2262-bp SOD7 promoter sequence was amplified using the primers SOD7P-F and SOD7P-R (Table 1). PCR products were cloned into pCR8/TOPO TA cloning vector. The SOD7 promoter was then subcloned into the binary vectors pGWB3 with the GUS gene to generate the transformation plasmid pSOD7:GUS. The plasmids 35S:GFP-SOD7, pSOD7:SOD7-GFP and pSOD7:GUS were introduced into Col-0 or sod7-ko1 ngal3ko1 plants using Agrobacterium tumefaciens GV3101, respectively, and transformants were selected on hygromycin (30 .mu.g/ml)-containing medium. The SOD7 cDNA was cloned into the Apal and Spel sites of the binary vector pER8 to generate a chemically inducible construct pER8-SOD7. The specific primers for the pER8-SOD7 construct were SOP7ER-F and SOD7ER-R. The plasmid pER8-SOD7 was introduced into Col-0 plants using Agrobacterium tumefaciens GV3101, and transformants were selected on hygromycin (30 .mu.g/ml)-containing medium. GUS staining Samples (pSOD7:GUS) were stained in a GUS staining solution (1 mM X-gluc, 50 Mm NaPO4 buffer, 0.4 mM each K3Fe(CN)6/K4Fe(CN)6, and 0.1% (v/v) Triton X-100) and incubated at 37.degree. C. for 3 hours. After GUS staining, chlorophyll was removed by 70% ethanol. RT-PCR and quantitative real-time RT-PCR. Total RNA was extracted from Arabidopsis seedlings using an RNAprep pure Plant kit (TIANGEN). mRNA was reverse transcribed into cDNA using SuperScriptIII reverse transcriptase (Invitrogen). cDNA samples were standardized on ACTIN2 transcript amount using the primers ACTIN2-F and ACTIN2-R (Table 1). Quantitative real-time RT-PCR analysis was performed with a Lightcycler 480 machine (Roche) using the Lightcycler 480 SYBR Green I Master (Roche). ACTIN2 mRNA was used as an internal control, and relative amounts of mRNA were calculated using the comparative threshold cycle method. The primers used for RT-PCR and quantitative real-time RT-PCR are described in Table 1.

[0177] The Chromatin Immunoprecipitation (ChIP) Assay

[0178] The chromatin immunoprecipitation (ChIP) assay was performed as described previously with minor modifications (Gendrel et al., 2005). Briefly, 35S:GFP and 35S:GFP-SOD7 transgenic seeds were grown on 1/2 MS plates for 10 days. The seedlings were cross-linked by 1% formaldehyde for 15 min in vacuum and stopped by 0.125 M Glycine. Samples were ground in liquid nitrogen, and nuclei were isolated. Chromatin was immunoprecipitated by anti-GFP (Roche, 11814460001) and protein A+G beads (Millpore Magna ChIP Protein A+G Magnetic Beads, 16-663). DNA was precipitated by glycogen, NaOAc and ethanol, washed by 70% ethanol, and dissolved in 60 .mu.l of water. Gene-specific primers (PF1-F, PF1-R, PF-2F, PF2-R, ACTIN7-ChIP-F, and ACTIN7-ChIP-R) were used to quantify the enrichment of each fragment (Table 1).

[0179] The DNA Electrophoretic Mobility Shift Assay (EMSA)

[0180] The coding sequence of SOD7 was cloned into the NdeI and BamHI sites of the pMAL-C2 vector to generate the construct MBP-SOD7. MBP-SOD7 fusion proteins were expressed in Escherichia coli BL21 (DE3) (Biomed) and purified by Amylose resins(New England Biolabs). The biotin-labeled and unlabeled probes were synthesized as forward and reverse strands. The forward and reverse strands were then incubated in a solution (50 mM Tris-HCl, 5 mM EDTA and 250 mM NaCl) at 95.degree. C. for 10 min and renatured to double stranded probes at room temperature. The gel-shift assay was performed according to the method described previously (Smaczniak et al., 2012).

[0181] Results

[0182] sod7-1D Suppresses the Seed Size Phenotype of Dal-1

[0183] We previously identified the ubiquitin receptor DA1 as a negative regulator of seed size in Arabidopsis (Li et al., 2008). The da1-1 mutant formed large seeds due to increased cell proliferation in the maternal integuments (Li et al., 2008; Xia et al., 2013). To identify novel components in the DA1 pathway or other seed size regulators, we initiated a T-DNA activation tagging screen for modifiers of da1-1 (Fang et al., 2012). A dominant suppressor of da1-1 (sod7-1D) was isolated from seeds produced from approximate 16,000 T1 plants (FIG. 1A). Seeds of the sod7-1D da1-1 double mutant were significantly smaller and lighter than da1-1 seeds (FIGS. 1A, E and F). The embryo constitutes the major volume of a mature seed in Arabidopsis. sod7-1D da1-1 embryos were smaller than da1-1 embryos (FIG. 1B). The size of sod7-1D da1-1 cotyledons was significantly reduced, compared with that of da1-1 cotyledons (FIG. 1G). In addition, sod7-1D da1-1 double mutant formed smaller leaves and flowers than da1-1 (FIGS. 1C and 1D). Thus, these results show that the sod7-1D mutation suppressed the seed and organ size phenotypes of da1-1.

[0184] sod7-1D Produces Small Seeds

[0185] We isolated the single sod7-1D mutant among F2 progeny derived from a cross between the wild type (Col-0) and sod7-1D da1-1. The sod7-1D seeds were significantly smaller and lighter than wild-type seeds (FIGS. 2A, B, G and H). We further isolated and visualized embryos from mature wild-type and sod7-1D seeds. The sod7-1D embryos were obviously smaller than wild-type embryos (FIGS. 2C and D). The changes in seed size were also reflected in the size of seedlings (FIGS. 2E and F). The 10-d old sod7-1D cotyledons were significantly smaller than wild-type cotyledons (FIGS. 2E, F and I). In addition, the sod7-1D mutants exhibited small leaves and flowers compared with the wild type. The decreased size of sod7-1D leaves and petals was not caused by smaller cells, indicating that the sod7-1D mutation results in a decrease in cell number. In fact, the average area of epidermal cells in sod7-1D petals was larger than that in wild-type petals, suggesting a possible compensation mechanism between cell number and cell size.

[0186] SOD7 Encodes a B3 Domain Transcriptional Repressor NGAL2

[0187] To determine whether the seed and organ size phenotypes of sod7-1D was caused by the T-DNA insertion, we firstly analyzed the genetic linkage of the mutant phenotypes with Basta resistance, which is conferred by the selectable marker of the activation tagging vector (Fan et al., 2009). In a T2 population, 181 plants with sod7-1D da1-1 phenotypes were resistant, whereas 55 plants with da1-1 phenotypes were sensitive, indicating that the insertion is cosegregated with the sod7-1D phenotypes. To clone the SOD7 gene, we isolated the T-DNA flanking sequences using thermal asymmetric interlaced PCR (Liu et al., 1995). DNA sequencing revealed that the T-DNA had inserted approximately 5.6 kb upstream of the At3g11580 and about 3.7 kb upstream of the At3g11590 gene (FIG. 3A). To determine which gene is responsible for the sod7-1D phenotypes, we examined the mRNA levels of these two genes. The mRNA of the At3g11590 gene accumulated at a similar level in sod7-1D da1-1 and da1-1, suggesting that At3g11590 is not the SOD7 gene (FIG. 3B). By contrast, expression level of the At3g11580 gene in sod7-1D da1-1 plants was dramatically higher than that in da1-1 plants, suggesting that At3g11580 is the SOD7 gene (FIG. 3B). To further confirm whether the sod7-1D phenotypes were caused by ectopic At3g11580 expression, we overexpressed the At3g11580 gene (35S:GFP-SOD7) in wild-type plants (Col-0) and isolated 37 transgenic plants. Most transgenic lines showed small seeds and organs (FIGS. 3D-F), similar to those observed in the sod7-1D single mutant, indicating that At3g11580 is the SOD7 gene. The SOD7 gene encodes a NGATHA like protein (NGAL2) containing a B3 DNA-binding domain and a transcriptional repression motif (FIG. 3C) (Alvarez et al., 2009; Ikeda and Ohme-Takagi, 2009; Trigueros et al., 2009). SOD7 belongs to the RAV gene family that consists of 13 members in Arabidopsis (FIG. 10) (Swaminathan et al., 2008). Several members of the RAV family contain the putative transcriptional repression motifs, including NGA1, NGA2, NGA3, NGA4, NGAL1, NGAL2/SOD7 and NGAL3 (FIG. 10) (Ikeda and Ohme-Takagi, 2009). The transcriptional repression motifs in NGA1, NGAL1 and NGAL2/SOD7 have been known to possess the repressive activity (Ikeda and Ohme-Takagi, 2009), indicating that they are transcriptional repressors. SOD7 exhibits the highest similarity to Arabidopsis NGAL3/DEVELOPMENT-RELATED PcG TARGET IN THE APEX 4 (DPA4) (FIG. 10), which has known roles in the regulation of leaf serrations (Engelhorn et al., 2012), but no previously identified function in seed size control.

[0188] Expression Pattern and Subcellular Localization of SOD7

[0189] To monitor SOD7 expression pattern during development, the pSOD7:GUS and pSOD7:SOD7-GFP vectors were constructed and transformed to wild-type plants, respectively. The tissue-specific expression patterns of SOD7 were examined using a histochemical assay for GUS activity. In seedlings, relatively higher GUS activity was detected in younger leaves than in older leaves (FIGS. 4A-C). In flowers, GUS activity was observed in sepals, petals, stamens and carpels (FIGS. 4D-K). GUS activity was stronger in younger floral organs than in older ones (FIGS. 4D-K). Expression of SOD7 was also detected in ovules (FIG. 4L). Thus, these analyses indicate that SOD7 is a temporally and spatially expressed gene. As SOD7 encodes a B3 domain transcriptional repressor, we speculated that SOD7 is localized in the nucleus. To determine subcellular localization of SOD7, we observed GFP inflorescence in pSOD7:SOD7-GFP transgenic plants. As shown in FIGS. 4M-O, GFP signal was only detected in nuclei. We also expressed a GFP-SOD7 fusion protein under the control of the 35S promoter in wild-type plants. Transgenic lines overexpressing GFP-SOD7 formed smaller seeds than the wild type (FIG. 3D), indicating that the GFP-SOD7 fusion protein is functional. As shown in FIGS. 4P-R, GFP fluorescence in 35S:GFP-SOD7 transgenic plants was exclusively observed in nuclei. Thus, these results show that SOD7 is a nuclear-localized protein.

[0190] SOD7/NGAL2 Acts Redundantly with NGAL3 to Control Seed Size

[0191] In order to further investigate the function of SOD7 in seed size control, we isolated T-DNA inserted loss-of-function mutants for SOD7 and NGAL3, the most closely related family member. sod7-ko1 (SM_3_34191) was identified with T-DNA insertion in the first exon of the SOD7 gene (FIG. 5A). ngal3-ko1 (SM_3_36641) had T-DNA insertion in the first exon of the NGAL3 gene (FIG. 5B). The T-DNA insertion sites were confirmed by PCR using T-DNA specific and flanking primers and sequencing PCR products. sod7-ko1 and ngal3-ko1 mutants had no detectable full-length transcripts of SOD7 and NGAL3, respectively. Seeds from sod7-ko1 and ngal3-ko1 mutants were slightly larger and heavier than seeds from wild-type plants (FIGS. 5C, G and H). The cotyledon area of sod7-ko1 and ngal3-ko1 mutants was increased, compared with that of the wild type (FIG. 5I). Considering that SOD7 shares the highest similarity with NGAL3, we speculated that SOD7 may act redundantly with NGAL3 to influence seed size. To test this, we generated the sod7-ko1 ngal3-ko1 double mutant. As shown in FIGS. 5C, D, G and H, the seed size and weight phenotypes of sod7-ko1 mutant were synergistically enhanced by the disruption of NGAL3, indicating that SOD7 functions redundantly with NGAL3 to control seed size. We further measured the cotyledon area of 10-d-old seedlings. A synergistic enhancement of cotyledon size of sod7-ko1 by the ngal3-ko1 mutation was also observed (FIG. 5I). In addition, the sod7-ko1 ngal3-ko1 double mutant formed larger leaves and flowers than their parental lines (FIGS. 5E and F; 11). Thus, these results indicate that SOD7 and NGAL3 act redundantly to control seed and organ growth.

[0192] SOD7 Acts Maternally to Control Seed Size

[0193] As the size of a seed is determined by the zygotic and/or maternal tissues (Garcia et al., 2005; Xia et al., 2013; Du et al., 2014), we asked whether SOD7 functions maternally or zygotically. We therefore performed reciprocal cross experiments between the wild type and sod7-ko1 ngal3-ko1. The effect of sod7-ko1 ngal3-ko1 on seed size was observed only when sod7-ko1 ngal3-ko1 was used as maternal plants (FIG. 6A). The size of seeds from sod7-ko1 ngal3-ko1 plants pollinated with wild-type pollen was similar to that from the self-pollinated sod7-ko1 ngal3-ko1 plants (FIG. 6A). By contrast, the size of seeds from wild-type plants pollinated with sod7-ko1 ngal3-ko1 mutant pollen was similar to that from the self-pollinated wild-type plants (FIG. 6A). These results indicate that sod7-ko1 ngal3-ko1 acts maternally to influence seed size. We further investigated the size of Col-0/Col-0 F2, Col-0/sod7-ko1 ngal3-ko1 F2, sod7-ko1 ngal3-ko1/Col-0 F2 and sod7-ko1 ngal3-ko1/sod7-ko1 ngal3-ko1 F2 seeds. As shown in FIG. 6B, sod7-ko1 ngal3-ko1/sod7-ko1 ngal3-ko1 F2 seeds were larger than wild-type seeds, while the size of Col-0/sod7-ko1 ngal3-ko1 F2 and sod7-ko1 ngal3-ko1/Col-0 F2 seeds was similar to that of wild-type seeds. Thus, these results indicate that the embryo and endosperm genotypes for SOD7 do not determine seed size, and SOD7 is required in the sporophytic tissue of the mother plant to control seed growth.

[0194] SOD7 Regulates Cell Proliferation in the Maternal Integuments

[0195] The reciprocal crosses showed that SOD7 functions maternally to influence seed size. The integuments surrounding the ovule are maternal tissues, which could set the growth potential of the seed coat after fertilization. Consistent with this idea, several studies showed that the integument size influences the final size of seeds in Arabidopsis (Garcia et al., 2005; Schruff et al., 2006; Adamski et al., 2009; Xia et al., 2013; Du et al., 2014). We therefore asked whether SOD7 acts through the maternal integuments to determine seed size. To test this, we characterized mature ovules of the wild type and sod7-ko1 ngal3-ko1. As shown in FIGS. 6C and D, the sod7-ko1 ngal3-ko1 ovules were obviously larger than wild-type ovules. The outer integument length of sod7-ko1 ngal3-ko1 ovules was significantly increased, compared with that of wild-type ovules (FIG. 6E). As the size of the integument is determined by cell proliferation and cell expansion, we examined the number and size of outer integument cells in wild-type and sod7-ko1 ngal3-ko1 ovules. As shown in FIG. 6F, the number of outer integument cells in sod7-ko1 ngal3-ko1 ovules was increased, compared with that in wild-type ovules. By contrast, the length of outer integument cells in sod7-ko1 ngal3-ko1 ovules was similar to that in wild-type ovules (FIG. 6G). These results showed that SOD7 is required for cell proliferation in the maternal integuments of ovules. After fertilization, cells in the integument mainly undergo expansion but still have division. We further examined the number and size of outer integument cells in wild-type and sod7-ko1 ngal3-ko1 seeds at 6 and 8 day after pollination (DAP). In wild-type seeds, the number of outer integument cells at 6 DAP was comparable with that at 8 DAP (FIG. 6F), indicating that cells in the outer integuments of wild-type seeds completely stop dividing by 6 DAP. Similarly, cells in the outer integuments of sod7-ko1 ngal3-ko1 seeds also cease division by 6 DAP. The number of outer integument cells in sod7-ko1 ngal3-ko1 seeds was significantly increased, compared with that in wild-type seeds (FIG. 6F). By contrast, the length of outer integument cells in sod7-ko1 ngal3-ko1 seeds was not increased in comparison to that in wild-type seeds (FIG. 6G). Thus, these analyses indicate that SOD7 is required for cell proliferation in the maternal integuments of ovules and developing seeds.

[0196] SOD7 Acts in a Common Pathway with KLU to Control Seed Size, but does so Independently of DA1

[0197] The Arabidopsis klu mutants formed small seeds due to the decreased cell proliferation in the integuments, while plants overexpressing KLU/CYP78A5 produced large seeds as a result of the increased cell proliferation in the integuments (Adamski et al., 2009), suggesting that SOD7 and KLU could function antagonistically in a common pathway to control seed growth. To test for genetic interactions between SOD7 and KLU, we generated the klu-4 sod7-ko1 ngal3-ko1 triple mutant and measured the size of seeds from wild-type, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 plants. As shown in FIGS. 7A and B, the average size and weight of klu-4 sod7-ko1 ngal3-ko1 seeds were similar to those of the klu-4 single mutant, indicating that klu-4 is epistatic to sod7-ko1 ngal3-ko1 with respect to seed size and weight. We further investigated the mature ovules from wild-type, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 plants. The outer integument length of klu-4 sod7-ko1 ngal3-ko1 ovules was comparable with that of klu-4 ovules (FIG. 7C). Similarly, the outer integument length of klu-4 sod7-ko1 ngal3-ko1 seeds was indistinguishable from that of klu-4 seeds at 8 DAP (FIG. 7C). In addition, the size of klu-4 sod7-ko1 ngal3-ko1 petals was similar to that of klu-4 petals).

[0198] Thus, these genetic analyses show that klu-4 is epistatic to sod7-ko1 ngal3-ko1 with respect to seed and organ size, indicating that SOD7 and KLU act antagonistically in a common pathway to control seed and organ growth. To further understand the cellular basis of epistatic interactions between SOD7 and KLU, we investigated the outer integument cell number of ovules and developing seeds from wild-type, klu-4, sod7-ko1 ngal3-ko1 and klu-4 sod7-ko1 ngal3-ko1 plants. The number of outer integument cells in klu-4 sod7-ko1 ngal3-ko1 ovules was similar to that in klu-4 ovules (FIG. 7D). Similarly, the number of outer integument cells in klu-4 sod7-ko1 ngal3-ko1 seeds was comparable with that in klu-4 seeds (FIG. 7D). These results indicate that klu-4 is epistatic to sod7-ko1 ngal3-ko1 with respect to the number of outer integument cells. We also observed that cells in the outer integuments of klu-4 and klu-4 sod7-ko1 ngal3-ko1 seeds were slightly longer than those in wild-type seeds, suggesting a possible compensation mechanism between cell proliferation and cell expansion. Together, these findings show that SOD7 functions antagonistically in a common pathway with KLU to control cell proliferation in the maternal integuments.

[0199] Considering that sod7-1D was identified as a suppressor of da1-1 in seed size, we further asked whether SOD7 and DA1 could act in the same genetic pathway. To test this, we measured the size of wild-type, da1-1, sod7-1D and sod7-1D da1-1 seeds. The genetic interaction between sod7-1D and da1-1 was essentially additive for seed size, compared with that of sod7-1D and da1-1 single mutants, indicating that SOD7 might function independently of DA1 to control seed size. We further crossed sod7-ko1 ngal3-ko1 with da1-1 and generated the sod7-ko1 ngal3-ko1 da1-1 triple mutant and measured its seed size. The genetic interaction between sod7-ko1 ngal3-ko1 and da1-1 was also additive for seed size, compared with their parental lines, further supporting that SOD7 functions to control seed growth separately from DA1.

[0200] SOD7 Directly Binds to the Promoter of KLU and Represses the Expression of KLU

[0201] Considering that SOD7 acts antagonistically in a common pathway with KLU to control seed size, we asked whether the transcription repressor SOD7 could repress the expression of KLU. We therefore investigated the expression of KLU in the chemically-inducible SOD7 (pER8-SOD7) transgenic plants. After the pER8-SOD7 transgenic plants were treated with the inducer (.beta.-estradiol), the expression of SOD7 was strongly induced at 4 and 8 hours (FIG. 8A). As expected, the expression of KLU was dramatically repressed at 4 and 8 hours (FIG. 8A). Thus, these results indicate that SOD7 represses the expression of KLU and also suggest that KLU might be a direct target of SOD7.

[0202] To determine whether SOD7 can directly bind to the promoter of the KLU gene, we performed a chromatin immunoprecipitation (ChIP) assay with 35S:GFP and 35:GFP-SOD7 transgenic plants. It has been reported that the CACCTG sequence is recognized by the B3 domain of RAV1, one member of the RAV family (Kagaya et al., 1999; Yamasaki et al., 2004). We therefore analyzed the promoter sequence of KLU and did not find an intact CACCTG sequence within 2 kb promoter region of KLU. However, we found a similar sequence (CACTTG) in the promoter region of KLU (FIG. 8B), which could be the potential SOD7-binding site. To test this, we examined the enrichment of a KLU promoter fragment (PF1) containing the CACTTG sequence by ChIP analyses and found that the fragment PF1 was strongly enriched in the chromatin-immunoprecipitated DNA with anti-GFP antibody (FIGS. 8B and C). By contrast, we did not detect significant enrichment of an ACTIN7 promoter sequence and the KLU promoter fragment PF2, which do not contain the CACTTG sequence (FIGS. 8B and C). This result shows that SOD7 associates with the promoter of KLU in vivo. We further expressed SOD7 as a MBP fusion protein (MBP-SOD7) and performed the DNA electrophoretic mobility shift assays (EMSA). As shown in FIGS. 8B and D, MBP-SOD7 was able to bind to the biotin-labeled probe A containing the CACTTG sequence, and the binding was reduced by the addition of an unlabeled probe A. By contrast, MBP-SOD7 failed to bind to a probe A-m with mutations in the CACTTG sequence (FIGS. 8B and D). Taken together, these results show that SOD7 directly binds to the promoter of KLU and represses KLU expression.

[0203] Discussion

[0204] Seed size is crucial for plant fitness and agricultural purposes, but little is known about the genetic and molecular mechanisms that set the final size of seeds in plants. In this study, we show that SOD7 acts maternally to control seed size by restricting cell proliferation in the integuments of ovules and developing seeds. SOD7 encodes a B3 domain transcriptional repressor NGAL2 and acts redundantly with its closest homolog NGAL3 to control seed size. Genetic analyses indicate that SOD7 functions in a common pathway with the maternal factor KLU to control seed growth, but does so independently of DA1. Further results reveal that SOD7 directly binds to the promoter region of KLU and represses KLU expression. Thus, our findings identify SOD7 as a negative factor for seed size and define the genetic and molecular mechanisms of SOD7 and KLU in seed size control.

[0205] SOD7 Acts Maternally to Regulate Seed Size

[0206] The sod7-1D gain-of-function mutant was identified as a suppressor of the large seed phenotype of da1-1. However, genetic analyses showed that SOD7 functions independently of DA1 to control seed growth. The sod7-1D single mutant produced small seeds and organs (FIG. 2), while the simultaneous disruption of SOD7 and the closely related family member NGAL3 resulted in large seeds and organs (FIG. 5), indicating that SOD7 is a negative regulator of seed and organ size. Several previous studies suggest that there is a possible link between seed size and organ growth. For instance, arf2, da1-1, da2-1 and eod3-1D mutants produced large seeds and organs (Schruff et al., 2006; Li et al., 2008; Fang et al., 2012; Xia et al., 2013), whereas klu and sod2/ubp15 mutants formed small seeds and organs (Anastasiou et al., 2007; Adamski et al., 2009; Du et al., 2014). However, seed size is not invariably associated with organ size. For example, eod8/med25 mutants with large organs formed normal-sized seeds (Xu and Li, 2011), while ap2 mutants with normal-sized organs produced large seeds (Jofuku et al., 2005; Ohto et al., 2005). Thus, these findings suggest that seeds and organs not only share common mechanisms but also possess distinct pathways to control their respective size.

[0207] Reciprocal cross experiments showed that SOD7 acts maternally to restrict seed growth, and the endosperm and embryo genotypes for SOD7 do not determine seed size (FIG. 6). The integuments surrounding the ovule are maternal tissues and form the seed coat after fertilization. Arabidopsis arf2, ap2, da1-1, da2-1 and eod3-1D mutants with large integuments formed large seeds (Jofuku et al., 2005; Ohto et al., 2005; Schruff et al., 2006; Li et al., 2008; Fang et al., 2012; Xia et al., 2013), while klu-4 and ubp15/sod2 mutants with small integuments produced small seeds (Adamski et al., 2009; Du et al., 2014), indicating that the maternal integuments are crucial for determining seed size in Arabidopsis. Consistent with this notion, mature eod7-ko1 ngal3-ko1 ovules were larger than wild-type ovules (FIGS. 6C and D). The outer integument length of eod7-ko1 ngal3-ko1 ovules and developing seeds was significantly increased, compared with that of wild-type ovules and seeds (FIGS. 6E and 7C). Considering that the maternal integument or seed coat not only acts as a protective structure but also restricts seed growth, the regulation of maternal integument size is one of important mechanisms for seed size control. The size of the integument is determined by cell proliferation and cell expansion; these two processes are assumed to be coordinated. The number of outer integument cells in sod7-ko1 ngal3-ko1 ovules and seeds was significantly increased, compared with that in wild-type ovules and seeds (FIG. 6F), indicating that SOD7 controls seed growth by limiting cell proliferation in the maternal integuments. Similarly, several mutants with the increased number of cells in the maternal integuments produced large seeds in Arabidopsis (Schruff et al., 2006; Li et al., 2008; Xia et al., 2013). By contrast, several other mutants with the decreased number of cells in the maternal integuments formed

[0208] small seeds in Arabidopsis (Adamski et al., 2009; Du et al., 2014). Considering that cells in the integuments mainly undergo expansion after fertilization (Garcia et al., 2005), it is possible that the number of cells in the integuments determines the growth potential of the seed coat after fertilization.

[0209] The Genetic and Molecular Mechanisms of SOD7 and KLU in Seed Size Control

[0210] The sod7-1D mutant had small seeds and organs (FIG. 2), as had been seen in klu mutants (Anastasiou et al., 2007; Adamski et al., 2009). KLU encodes a cytochrome P450 CYP78A5 that has been proposed to generate mobile plant-growth substances (Anastasiou et al., 2007; Adamski et al., 2009). KLU regulates seed size by promoting cell proliferation in the maternal integuments of ovules (Anastasiou et al., 2007; Adamski et al., 2009). By contrast, SOD7 acts maternally to control seed size by limiting cell proliferation in the integuments of ovules and developing seeds (FIG. 6). These results suggest that SOD7 could function antagonistically in a common pathway with KLU to control seed size. In our growth conditions, klu-4 formed slightly smaller seeds than the wild type due to the decreased cell number and the slightly increased cell length in the integuments of developing seeds (FIGS. 7A and D), suggesting a possible compensation mechanism between cell proliferation and cell expansion in klu-4 integuments. Importantly, our genetic analyses showed that klu-4 is epistatic to sod7-ko1 ngal3-ko1 with respect to seed and organ size (FIGS. 7A and B). klu-4 is also epistatic to sod7-ko1 ngal3-ko1 for the outer integument length (FIG. 7C). Further results revealed that the number of cells in the outer integuments of klu-4 sod7-ko1 ngal3-ko1 ovules and developing seeds was similar to that of k/u-4 ovules and developing seeds (FIG. 7D). Thus, these genetic results demonstrate that SOD7 act in a common pathway with KLU to control seed size by regulating cell proliferation in the maternal integuments.

[0211] SOD7 encodes a B3 domain transcriptional repressor NGAL2 that is localized in nuclei of Arabidopsis cells (FIGS. 4M-R). Thus, it is possible that SOD7 could directly bind to the promoter of KLU and repress KLU expression. Supporting this idea, the inducible expression of SOD7 resulted in a strong reduction of KLU expression (FIG. 8A). Our ChIP-qPCR data showed that SOD7 associates with the promoter region of KLU in vivo (FIGS. 8B and C). EMSA experiments revealed that SOD7 directly binds to the CACTTG sequence in the promoter of the KLU gene (FIGS. 8B and D). Thus, these results illustrate that SOD7 directly targets the promoter region of KLU and represses the expression of KLU, thereby determining seed size. Taken together, these findings reveal the genetic and molecular mechanisms of SOD7 and KLU in regulating Arabidopsis seed size.

[0212] For many plants, the seeds are the main product to be harvested, and an increase in seed size would be beneficial for growers. In this study, we identify SOD7 as a negative regulator of seed size, and demonstrate that SOD7 acts in a common genetic pathway with KLU to control seed size. Our current knowledge of SOD7 functions suggests that the SOD7 gene (and its homologs in other plant species) could be used to engineer large seed size in crops. Considering that crop plants have undergone selection for large seed size during domestication (Fan et al., 2006; Song et al., 2007; Gegas et al., 2010), it will be a worthwhile challenge to know whether beneficial alleles of the SOD7 gene have already been utilized by plant breeders.

[0213] Knockout Experiments in Rice Using Genome Editing

[0214] Genome editing experiments to knock out os11g01560000 and/or Os12g0157000 in rice are being carried out using the crisper-cas9 system. Four vectors, each with two recognition (CRISPR target) sites, have been constructed, to achieve these knock outs, as described in FIG. 14. In summary, the vectors were obtained as follows:

[0215] 1. The target sites were identified. The target site should be (or approximately so) 20 nucleotides before a NGG sequence, N being for any nucleotide. The target sequence was then evaluated using the website: http://cbi.hzau.edu.cn/crispr/help.php (incorporated herein by reference). Of note, the target site should be unique in the genome.

[0216] 2. Using overlap PCR, the target sequence is linked with the U6 sequence, as shown in FIG. 14. U6 is for transcriptional activity.

[0217] 3. Using infusion technology we connected the U6-guide-gRNA scaffold fragment to the vector pMDC99-cas9 to obtain the pMDC99-cas9-U6-guide-gRNA scaffold constructs. These constructs were named zyy1,zyy2, zyy3,zyy4. The full sequences of these constructs are represented in SEQ ID NO: 155, 156, 157 and 158 respectively. Each construct contains two recognition sites, which are highlighted in the sequence information, and are represented separately as SEQ ID Nos 159, 160, 161, 162 and 163.

[0218] 4. We then transformed these constructs into Agrobacteria and used an Agrobacteria mediated method to transform rice and obtain gene-edited rice. Transformation of plants is a routine technique that is well known to the skilled person. Nonetheless, a brief outline of transformation techniques is provided above.

[0219] Knock out lines are being analysed to assess the phenotype.

TABLE-US-00001 TABLE 1 Primers used in this study Promer Name Promer Sequences Primers tor T-DNA tdenttttcatton SM_3_34191-LP ACCATGACATTCGAGGTTCAC (SEQ ID NO. 8) SM_3_34191-RP ATCACCACCAAAACGACGTAG (SEQ ID NO. 9) SM_3_36641-RP TACGTCATGCTTCAAATCGTG (SEQ ID NO. 10) SM_3_36641-RP AGGACACGAACAATTCATTCG (SEQ ID NO. 11) Spm32 TACGAATAAGAGCGTCCATTTTAGAGTGA (SEQ ID NO. 12) SM_3_39145-LP ACCCAAAGAACAGCAATCATG (SEQ ID NO. 13) SM_3_39145-RP AAAACACTCCGCCATTAAACC (SEQ ID NO. 14) Primers tor TAIL-PCR OJF22 CGAGTATCAATGGAAACTTAACCG (SEQ ID NO.15) OJF23 AACGGAGAGTGGCTTGAGAT (SEQ ID NO. 16) OJF24 TGGCCCTTATGGTTTCTGCA (SEQ ID NO. 17) AD1 NTCGA(G/C)T(NT)T(G/C)G(A/T)GTT (SEQ ID NO. 18) Primers tor Constructs SOD7CDS-F ATGTCAGTCAACCATTACCAC (SEQ ID NO. 19) SOD7CDS-R CAGGTAGGAGATGGACGAGGTTGA (SEQ ID NO. 20) SOD7G-F TGAGAGGAACCATTTCTTAGAGG (SEQ ID NO. 21) SOD7G-R ACCTCGTCCATCTCCTACCTGC (SEQ ID NO. 22) SOD7P-F AAACACGTCAAATATAACGAAT (SEQ ID NO. 23) SOD7P-R CTTTTTTTTGGTTTCTTGGAGTGAGAGAGAGAG (SEQ ID NO. 24) SOD7-ER-F AGTCTGGGCCCATGTCAGTCAACCATTAC (SEQ ID NO. 25) SOD7-ER-R GCGACTAGTTTATAAAAGAGTTAAAATTA (SEQ ID NO. 25) MBP-SOD7-FP CGGGATCCTCAGTCAACCATTACC (SEQ ID NO. 27) MBP-SOD7-RP ACTAGTCGACTCAACCTCGTCCATCTCC (SEQ ID NO. 28) Primers tor RT-PCR and qRT-PCR ACTIN2-F GAAATCACAGCACTTGCACC (SEQ ID NO. 29) ACTIN2-R AAGCCTTTGATCTTGAGAGC (SEQ ID NO. 30) SOD7-EX-F GCGACGACGGAGAAAGGG (SEQ ID NO. 31) SOD7-EX-R ACGACGGCGCCATAGTGT (SEQ ID NO. 32) NGAL3-EX-F TTTGAAGACGAGTCAGGCAAGT (SEQ ID NO. 33) NGAL3-EX-R TACGGCGGCTCCATAGTGGG (SEQ ID NO. 34) SOD7-q-FP GTATTGGAGCGGCTTGACTACACC (SEQ ID NO. 35) SOD7-q-RP GACGGCATCACCATGACATTCG (SEQ ID NO. 36) KLU-q-FP TGATTCTGACATGATTGCTGTTCT (SEQ ID NO. 37) KLU-q-RP TCGCAACTGTATCTGTCCCTCTA (SEQ ID NO. 38) Primers tor ChIP assay ACTIN7-ChIP-FP CGTTTCGCTTTCCTTAGTGTTAGCT (SEQ ID NO. 29) ACTIN7-ChIP-RP AGCGAACGGATCTAGAGACTCACCTTG (SEQ ID NO. 40) PF1-F CAGGCCTAAGCCTAACAGTAGAC (SEQ ID NO. 41) PF1-R TGTACTAGGATTTATTTACGTAG (SEQ ID NO. 42) PF2-F TATTGTTCATAGAAACCCTGCAAA (SEQ ID NO. 43) PF2-R AGTCAATGGTTTAATGGCGGAGTG (SEQ ID NO. 44) Probes tor EMSA A-Btottn-FP TTCTACTACACTTGCTCTCTGTA (SEQ ID NO. 45) A-Btottn-RP TACAGAGAGCAAGTGTAGTAGAA (SEQ ID NO. 46) A-Btottn-m-FP TTCTACTAACACCTCTCTCTGTA (SEQ ID NO. 47) A-Btottn-m-RP TACAGAGAGAGGTGTTAGTAGAA (SEQ ID NO. 48)

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SEQUENCE INFORMATION

TABLE-US-00002

[0259] Identtty of homologs to NGAL2 is tndtcated AtSOD7 nucleic acid SEQ ID NO. 1 (cDNA) At3g11580 ATGTCAGTCAACCATTACCACAACACTCTCTCGTTGCATCATCACCACCAAAACGACGTAGCTATAGCACAACG- AGAGTCTTTGTTC GAGAAATCACTCACACCAAGCGACGTCGGAAAGCTAAACCGCTTAGTCATACCAAAACAACACGCCGAGAAATA- CTTCCCTCTCAAT AATAATAATAATAATGGCGGCAGCGGAGATGACGTGGCGACGACGGAGAAAGGGATGCTTCTTAGCTTCGAGGA- TGAGTCAGGCAAG TGTTGGAAATTCAGATACTCTTATTGGAACAGTAGCCAAAGCTACGTGTTGACCAAAGGATGGAGCAGGTACGT- CAAAGACAAACAC CTCGACGCAGGCGACGTTGTTTTCTTTCAACGTCACCGTTTTGATCTCCATAGACTCTTCATTGGCTGGCGGAG- ACGCGGTGAAGCT TCTTCCTCTCCCGCTGTCTCCGTTGTGTCTCAAGAAGCTCTAGTTAATACGACGGCGTATTGGAGCGGCTTGAC- TACACCTTATCGT CAAGTACACGCGTCAACTACTTACCCTAATATTCACCAAGAGTATTCACACTATGGCGCCGTCGTTGATCATGC- TCAGTCGATACCA CCGGTGGTCGCAGGTAGCTCGAGGACGGTGAGGCTTTTTGGCGTGAACCTCGAATGTCATGGTGATGCCGTCGA- GCCACCACCGCGT CCTGATGTCTATAATGACCAACACATTTACTATTACTCAACTCCTCATCCCATGAATATATCATTTGCTGGGGA- AGCATTGGAGCAG GTAGGAGATGGACGAGGTTGA AtSOD7 nucleic acid SEQ ID NO. 2 (genomic DNA). ttgtttcggctatttgttatactattgttataacagtcacaagacttgacctcaacgaaaacttttacaaaacg- tgaattggaaatt tttacaaaatatgctcttaatcgttaatgcttcccaattaggtgagttaaattgtgagaggaaccatttcttag- aggaaatggttca tgaaaacaaatatgaaatagtatcactagtcttagttttgcgagaaaattaggaaaaatagaaacgtgtaagca- ccaatgatattcc tgaaagcacgtgacagatatttcatgatcctataattaacaagtgataaagatattaaataaaattaacgatac- ttgagaaattcgt caaataaaatagaagaggaccactcacgtaaccatttgcacgtcccattgatttttgtggtagacttggtatgt- tatattacttata ttcacagaattatatacgaaactcacgacttaagatgcacggtaataactacagatggaaatttacccatcaaa- caagaaaacaaca tttactcaagcatctagctagaccaaaatgtttgtttacttgttgacttgcgatccatagatatattagttaga- actttttcttcta caattgatcaaatgtttcacactgttctcaatttctcatctagattcatgacttatatgtttggtcaaatatca- cagcttgatgagc attaaatagcgtcgaagtataggatggttacgttgttcaatattgtaaaggaaaaaaagagaaagagtgccaaa- aggtcaagtcgat ttcacaaataaatcttgaagtctttatccctctcgattataaaatgattaggaaaagaaaaagagagaataaaa- tgtagataaagag aaagagaaagagagagaggaacataagggatggtatgaagtagaagtgaagatgcatgcgatggtgtgtcggaa- aggcaaagcacat gctacacaacttgagcttctcacttgcgtcagggataagtatcctctgtaccttcttacttttgcgtaatatgt- accacctcacttc tcaaccgtttgatctttaatccttcattatttcttcattaccttctctttttgtttttgttttcgttttcaatt- tctcatagattca tttacaaactaaatatcataggaaggtgttatctctagttaatttcttatcctactttaacaaaatttaattgt- caaaagattattt ttacgtttatagacaaaagatactgacacatcaattccacgaaccaaatggttgagaaaaacaaaacgactatc- tttgtcttgcaaa taaattaatggcagttagtaagattctcagctgaaaattcatacaagagtaaatgatcaaataaccatttatga- gagaaatttaatc cttcagaaaccaatgaggatctgatcaagtaattgcaaaccacatgagtccatgataaaggattgtttgactta- cgcaatccacata tttatggctgcttgatatgtaaggtttatctgctttgacagtctatagaatcttgctaatcaatacgtcatatc- cggtgaatactga aacttttttaattaagaaaacacaaatcatcttttctccggaggatttcgaatttagttccggcaatgctgaaa- taacatatgttga acttataacattccaagacatcaaattttactaatatataaataattacatattcttcttctacatgatcaaaa- ccttttcaacttt aattaaagggttacgtcgcggcgttttgtgtggcttactctttttttacactataactatagaacactcgtgga- tccaatgccgttt aggacaagattttatcagacgagaaaaaaaaaaacaataccacatttttaaatatatatggattatggactgca- acaacaatataga aaagaagagaaaaaaataaaaataatgattgaaaggaaatatcatcacgcaaaaccttaaaagtactatcggta- tcgtgtcgtcctc tcctcatcaaatagttcccacagttttcacatcaatttaaccattttcaatttttttcactctctgtctctctc- ctttgtataatac tatattagtaccattacccatctctctttcaccaccaaaccaacacctgcaaatcctctctctctctctcactc- caagaaaccaaaa aaaaagATGTCAGTCAACCATTACCACAACACTCTCTCGTTGCATCATCACCACCAAAACGACGTAGCTATAGC- ACAACGAGAGTCT TTGTTCGAGAAATCACTCACACCAAGCGACGTCGGAAAGCTAAACCGCTTAGTCATACCAAAACAACACGCCGA- GAAATACTTCCCT CTCAATAATAATAATAATAATGGCGGCAGCGGAGATGACGTGGCGACGACGGAGAAAGGGATGCTTCTTAGCTT- CGAGGATGAGTCA GGCAAGTGTTGGAAATTCAGATACTCTTATTGGAACAGTAGCCAAAGCTACGTGTTGACCAAAGGATGGAGCAG- GTACGTCAAAGAC AAACACCTCGACGCAGGCGACGTTGTTTTCTTTCAACGTCACCGTTTTGATCTCCATAGACTCTTCATTGGCTG- GCGGAGACGCGGT GAAGCTTCTTCCTCTCCCGCTGTCTCCGTTGTGTCTCAAGAAGCTCTAGTTAATACGACGGCGTATTGGAGCGG- CTTGACTACACCT TATCGTCAAGTACACGCGTCAACTACTTACCCTAATATTCACCAAGAGTATTCACACTATGgtaaattcaaacc- ctttatttcctct tttgttttttctttctctcttatctatatgtcagatttatactcctctctgttctcttttaagatttgtctttt- tcataaaaataga tgattcgtaatttgtattgcatatttacatgttctcttaaaaaaagtaatagagattaatattttatgcatggt- attttagattatc tgcctactttatatggtagtaaacaagaacattcatcthatttgOttataaacaaaatatgagaatttttaaag- gttagggcaagca cttggaaagctcaaccattttagttagctggtggaatatctttcttataaaaagcaaatgagttatctaaaact- atatgacaattat tttagttgcgtgtgtaatgtatataaaataacaacatgaaataacattttgtcttttatttttgtcattcttat- tatttaattttgg acccgacaatttcaaataatcttctccaagttgtaactaatccgttacatgcgcgtgaggagaaccgtccaatc- cacttagactaac gtgccctttatttcttccttttaattctatgttaaaaaaacaatttaactaaaagatgcgcacgtgtcttgacg- gtggaaaaaaatt gtagGCGCCGTCGTTGATCATGCTCAGTCGATACCACCGGTGGTCGCAGGTAGCTCGAGGACGGTGAGGCTTTT- TGGCGTGAACCTC GAATGTCATGGTGATGCCGTCGAGCCACCACCGCGTCCTGATGTCTATAATGACCAACACATTTACTATTACTC- AACTCCTCATCCC ATGgtaaatattttttttttttacatttttgtcagattcaaatttttgcttacgtatgatataattattaaaca- gatgtcgtggctg tttctcgagacgagacagatgaaaattagtaattttaaaatagacctgaaagagatttttatgtttaataaatt- atataaaggagga atcagagagaataatactatacacttgactgtaaaaccacatggccaatttggtttttatttgattactttgat- ttgttttgtttac tcttttgtctctgtagcctccttttgttcattaattaatatcagccgtaagtatatagtttcctgtgaaaacag- tctctattttggt tttactattctaatttgttaggcaccgtcagttttttttgtgaaaccaaattattgactaataagctggaaagc- aaaactgactaaa agcattacaaacttatcaatgacataagttttgaatttattaccatgttttgtaatgttcagatataatttgaa- atgcttagaatta tatatttgtatacttaaattaatgaaataaagtgaatactaaagatagttttatttttcatattattctataca- attcggtgtacaa tttgtttttgatgataataaaaataataaaattgcgtgttggaattgtgaaacagAATATATCATTTGCTGGGG- AAGCATTGGAGCA GGTAGGAGATGGACGAGGT AtNGAL2 SEQ ID NO.3 (protein encoded by AtSOD7).. MSVNHYHNTLSLHHHHQNDVAIAQRESLFEKSLTPSDVGKLNRLVIPKQHAEKYFPLNNNNNNGGSGDDVATTE- KGMLLSFEDESGK CWKFRYSYWNSSQSYVLTKGWSRYVKDKHLDAGDVVFFQRHRFDLHRLFIGWRRRGEASSSPAVSVVSQEALVN- TTAYWSGLTTPYR QVHASTTYPNIHQEYSHYGAVVDHAQSIPPVVAGSSRTVRLFGVNLECHGDAVEPPPRPDVYNDQHIYYYSTPH- PMNISFAGEALEQ VGDGRG AtNGAL3 nucleic acid sequence SEQ ID NO. 4 (cDNA) at5g06250 ATGTCAGTCAACCATTACTCCACAGACCACCACCACACTCTCTTGTGGCAGCAACAGCAACACCGCCACACCAC- CGACACATCGGAG ACAACCACCACCGCCACATGGCTCCACGACGACCTAAAAGAGTCACTCTTCGAGAAGTCTCTCACACCAAGCGA- CGTCGGGAAACTC AACCGCCTCGTCATACCAAAACAACACGCAGAGAAATACTTCCCTCTCAATGCCGTCCTAGTCTCCTCTGCTGC- TGCTGACACGTCA TCTTCGGAGAAAGGGATGCTTCTAAGCTTTGAAGACGAGTCAGGCAAGTCATGGAGGTTCAGATACTCTTACTG- GAACAGCAGTCAA AGCTATGTCTTGACTAAAGGATGGAGCAGATTTGTCAAAGACAAACAGCTCGATCCAGGCGACGTTGTTTTCTT- CCAACGACACCGT TCTGATTCTAGGAGACTCTTCATTGGCTGGCGCAGACGTGGACAAGGCTCCTCATCCTCCGTCGCGGCCACTAA- CTCCGCCGTGAAT ACGAGTTCTATGGGAGCTCTTTCTTATCATCAAATCCACGCCACTAGTAATTACTCTAATCCTCCCTCTCACTC- AGAGTATTCCCAC TATGGAGCCGCCGTAGCAACAGCGGCTGAGACTCACAGCACACCGTCGTCTTCCGTCGTCGGGAGCTCAAGGAC- GGTGAGGCTTTTC GGTGTGAATCTGGAGTGTCAAATGGATGAAAACGACGGAGATGATTCTGTTGCAGTTGCCACCACCGTTGAATC- TCCCGACGGTTAC TACGGCCAAAACATGTACTATTATTACTCTCATCCTCATAACATGGTAATTTTAACTCTTTTATAA AtNGAL3 amtno acid SEQ ID NO. 5 MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNRLVIPKQHAEKYFPL- NAVLVSSAAADTS SSEKGMLLSFEDESGKSWRFRYSYWNSSQSYVLTKGWSRFVKDKQLDPGDWFFQRHRSDSRRLFIGWRRRGQGS- SSSVAATNSAVNT SSMGALSYHQIHATSNYSNPPSHSEYSHYGAAVATAAETHSTPSSSWGSSRTVRLFGVNLECQMDENDGDDSVA- VATTVESPDGYYG QNMYYYYSHPHNMVILTLL Oryza sativa Os12g0157000 LOC_Os12g06080.1 Cover 73% identity 53% SEQ ID NO: 49 MAMHAGHAWWGVAMYTNHYHHHYRHKTSDVGKNRVKHARYGGGDSGKGSDSGKWRRYSYWTSSSYVTKGWSRYV- KKRDAGDVVHRVR GGAADRGCRRRGSAAAVRVTANGGWSMCYSTSGSSYDTSANSYAYHRSVDDHSDHAGSRADAKSSSAASASRRR- GVNDCGADATAMY GYMHHSYAAVSTVNYWSV CDS SEQ ID NO: 50 ATGGCCATGCACCCTCTCGCCCAGGGGCACCCCCAGGCGTGGCCATGGGGTGTAGCCATGTACACCAACCTGCA- CTACCACCACCAC TACGAGAGGGAGCACCTGTTCGAGAAGCCGCTGACGCCGAGCGACGTCGGCAAGCTCAACAGGCTGGTGATCCC- CAAGCAGCACGCC GAGAGGTACTTCCCGCTCGGCGGCGGCGACTCCGGTGAGAAGGGCCTCCTCCTCTCCTTCGAGGACGAGTCCGG- CAAGCCATGGCGG TTCCGCTACTCCTACTGGACCAGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTACGTCAAGGAGAA- GCGCCTCGACGCC GGCGACGTCGTCCACTTCGAGCGCGTCCGCGGCCTCGGCGCCGCCGACCGCCTCTTCATCGGCTGCAGGCGCCG- CGGCGAGAGCGCG CCCGCGCCGCCGCCCGCCGTTCGCGTCACGCCGCAGCCGCCTGCCCTCAACGGCGGCGAGCAGCAGCCGTGGAG- CCCAATGTGTTAC AGCACGTCGGGCTCGTCCTACGACCCTACCAGCCCTGCCAATTCATATGCCTACCATCGCTCCGTAGACCAAGA- TCACAGCGACATA CTACACGCAGGAGAGTCGCAGAGAGAAGCAGACGCCAAGAGCAGCAGCGCGGCGTCGGCGCCGCCGCCGTCGAG- GCGGCTCAGGCTG TTCGGCGTTAACCTCGACTGCGGCCCGGAGCCGGAGGCGGATCAGGCGACGGCAATGTACGGCTACATGCACCA- CCAGAGCCCCTAC GCCGCAGTGTCTACAGTGCCAAATTACTGGTCAGTATTTTTTCAGTTTTAA Os11g0156000 LOC_Os11g05740.1 Cover 81% identity 47% SEQ ID NO: 51 MAMNHPLFSQEQPQSWPWGVAMYANFHYHHHYEKEHMFEKPLTPSDVGKLNRLVIPKQHAERYFPLGAGDAADK- GLILSFEDEAGAP WRFRYSYVVTSSQSYVLTKGWSRYVKEKRLDAGDVVHFERVRGSFGVGDRLFIGCRRRGDAAAAQTPAPPPAVR- VAPAAQNAGEQQP WSPMCYSTSGGGSYPTSPANSYAYRRAADHDHGDMHHADESPRDTDSPSFSAGSAPSRRLRLFGVNLDCGPEPE- ADTTAAATMYGYM HQQSSYAAMSAVPSYWGNS CDS SEQ ID NO: 52 ATGGCCATGAACCACCCTCTCTTCTCCCAGGAGCAACCCCAGTCCTGGCCATGGGGTGTGGCCATGTACGCCAA- CTTCCACTACCAC CACCACTACGAGAAGGAGCACATGTTTGAGAAGCCCCTGACGCCCAGTGACGTGGGGAAGCTGAACCGGCTGGT- GATCCCCAAGCAG CACGCCGAGAGGTACTTCCCCCTCGGCGCCGGCGACGCCGCCGACAAGGGCCTGATCCTGTCGTTCGAGGACGA- GGCCGGCGCGCCG TGGCGGTTCAGGTACTCCTACTGGACGAGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTACGTCAA- GGAGAAGCGCCTC GACGCCGGCGACGTCGTGCACTTCGAGAGGGTGCGCGGCTCCTTCGGCGTCGGCGACCGTCTCTTCATCGGCTG- CAGGCGCCGCGGC GACGCCGCCGCCGCGCAAACACCCGCACCGCCGCCCGCCGTGCGCGTCGCCCCGGCTGCACAGAACGCCGGCGA- GCAGCAGCCGTGG AGCCCAATGTGTTACAGCACGTCGGGCGGCGGCTCATACCCTACCAGCCCAGCCAACTCCTACGCCTACCGCCG- CGCAGCAGATCAT GATCACGGGGACATGCACCATGCAGACGAGTCTCCGCGCGACACGGACAGCCCAAGCTTCAGTGCAGGCTCGGC- GCCATCGAGGCGG CTCAGGCTGTTCGGCGTCAACCTCGACTGCGGGCCAGAGCCGGAGGCAGACACCACGGCAGCGGCAACAATGTA- CGGCTACATGCAC CAGCAGAGCTCCTATGCTGCCATGTCTGCAGTACCCAGTTACTGGGGCAATTCATAA Os02g0683500 LOC_Os02g45850 Cover 47% identity 62% SEQ ID NO: 53 MEFTTSSRFSKEEEDEEQDEAGRREIPFMTATAEAAPAPTSSSSSPAHHAASASASASASGSSTPFRSDDGAGA- SGSGGGGGGGGEA EVVEKEHMFDKVVTPSDVGKLNRLVIPKQYAEKYFPLDAAANEKGLLLNFEDRAGKPWRFRYSYWNSSQSYVMT- KGWSRFVKEKRLD AGDTVSFSRGIGDEAARHRLFIDWKRRADTRDPLRLPRGLPLPMPLTSHYAPWGIGGGGGFFVQPSPPATLYEH- RLRQGLDFRAFNP AAAMGRQVLLFGSARIPPQAPLLARAPSPLHHHYTLQPSGDGVRAAGSPVVLDSVPVIESPTTAAKRVRLFGVN- LDNPHAGGGGGAA AGESSNHGNALSLQTPAWMRRDPTLRLLELPPHHHHGAESSAASSPSSSSSSKRDAHSALDLDL CDS SEQ ID NO: 54 ATGGAGTTCACTACAAGCAGTAGGTTTTCTAAAGAAGAGGAGGACGAGGAGCAGGATGAGGCGGGAAGGCGAGA- GATCCCCTTCATG ACGGCCACGGCCGAAGCCGCGCCTGCGCCCACGTCGTCGTCGTCGTCTCCTGCTCATCACGCGGCTTCCGCGTC- GGCGTCGGCGTCT

GCGTCAGGGAGCAGCACTCCCTTTCGCTCCGACGATGGCGCCGGGGCGTCTGGGAGCGGCGGCGGCGGCGGCGG- CGGCGGAGAAGCG GAGGTGGTGGAGAAGGAGCACATGTTCGACAAGGTGGTGACGCCGAGCGACGTTGGGAAGCTGAACCGGCTGGT- GATCCCGAAGCAG TACGCCGAGAAGTACTTCCCGCTGGACGCGGCGGCGAACGAGAAGGGCCTCCTGCTCAACTTCGAGGACCGCGC- GGGGAAGCCATGG CGGTTCCGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTGATGACCAAGGGGTGGAGCCGCTTCGTCAAGGA- GAAGCGCCTCGAC GCCGGGGACACCGTCTCCTTCTCCCGCGGCATCGGCGACGAGGCGGCGCGGCACCGCCTCTTCATCGACTGGAA- GCGCCGCGCCGAC ACCCGCGACCCGCTCCGGCTGCCCCGCGGGCTGCCGCTCCCGATGCCGCTCACGTCGCACTACGCCCCGTGGGG- GATCGGCGGCGGA GGGGGATTCTTCGTGCAGCCCTCGCCGCCGGCCACGCTCTACGAGCACCGCCTCAGGCAAGGCCTCGACTTCCG- CGCCTTCAACCCC GCCGCCGCGATGGGGAGGCAGGTCCTCCTGTTCGGCTCGGCGAGGATTCCTCCGCAAGCACCACTGCTGGCGCG- CGCGCCGTCGCCG CTGCACCACCACTACACGCTGCAGCCGAGCGGCGATGGTGTAAGGGCGGCGGGCTCACCGGTGGTGCTCGACTC- GGTTCCGGTCATC GAGAGCCCCACGACGGCCGCGAAGCGCGTGCGGCTGTTCGGCGTGAACCTCGACAACCCGCATGCCGGCGGCGG- CGGCGGCGCCGCC GCCGGCGAGTCGAGCAATCATGGCAATGCACTGTCATTGCAGACGCCCGCGTGGATGAGGAGGGATCCAACACT- GCGGCTGCTGGAA TTGCCTCCTCACCACCACCATGGCGCCGAGTCGTCCGCTGCATCGTCTCCGTCGTCGTCGTCTTCCTCCAAGAG- GGACGCGCATTCG GCCTTGGATCTCGATCTGTAG os04g0581400 LOC_Os04g49230 Cover 46% identity 64% CDS SEQ ID NO: 55 ATGGAGTTTGCTACAACGAGTAGTAGGTTTTCCAAGGAAGAGGAGGAGGAGGAGGAAGGGGAACAGGAGATGGA- GCAGGAGCAGGAT GAAGAGGAGGAGGAGGCGGAGGCCTCGCCCCGCGAGATCCCCTTCATGACGTCGGCGGCGGCGGCGGCCACCGC- CTCATCGTCCTCC CCGACATCGGTCTCCCCTTCCGCCACCGCTTCCGCGGCGGCGTCCACGTCGGCGTCGGGCTCTCCCTTCCGGTC- GAGCGACGGTGCG GGAGCGTCGGGGAGTGGCGGCGGCGGTGGCGGCGAGGACGTGGAGGTGATCGAGAAGGAGCACATGTTCGACAA- GGTGGTGACGCCG AGCGACGTGGGGAAGCTGAACCGGCTGGTGATCCCGAAGCAGCACGCCGAGAAGTACTTCCCGCTGGACTCGGC- GGCGAACGAGAAG GGCCTTCTCCTCAGCTTCGAGGACCGAACCGGCAAGCTATGGCGCTTCCGCTACTCCTACTGGAACAGCAGCCA- GAGCTACGTCATG ACCAAGGGTTGGAGCCGCTTCGTCAAGGAGAAGCGCCTCGACGCCGGGGACACCGTCTCCTTCTGCCGCGGCGC- CGCCGAGGCCACC CGCGACCGCCTCTTCATCGACTGGAAGCGCCGCGCCGACGTCCGCGACCCGCACCGCTTCCAGCGCCTACCGCT- CCCCATGACCTCG CCCTACGGCCCGTGGGGCGGCGGCGCGGGCGCTTCTTCATGCCGCCCGCGCCGCCCGCCACGCTCTACGAGCAT- CACCGCTTTCGCC AGGGCTTCGACTTCCGCAACATCAACCCCGCTGTGCCGGCGAGGCAGCTCGTCTTCTTCGGCTCCCCAGGGACG- GGGATTCATCAGC ACCCGCCCTTGCCACCGCCGCCGTCGCCACCTCCGCCTCCTCACCAACTCCACATTACGGTGCACCACCCGAGC- CCCGTAG SEQ ID NO: 56 MEFATTSSRFSKEEEEEEEGEQEMEQEQDEEEEEAEASPREIPFMTSAAAAATASSSSPTSVSPSATASAAAST- SASGSPFRSSDGA GASGSGGGGGGEDVEVIEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDSAANEKGLLLSFEDRTGKLWRF- RYSYWNSSQSYVM TKGWSRFVKEKRLDAGDTVSFCRGAAEATRDRLFIDWKRRADVRDPHRFQRLPLPMTSPYGPWGGGAGASSCRP- RRPPRSTSITAFA RASTSATSTPLCRRGSSSSSAPQGRGFISTRPCHRRRRHLRLLTNSTLRCTTRAP Os03g0120900 LOC_Os03g02900 Cover 47% identity 63% CDS SEQ ID NO: 57 ATGGAGTTCATCACGCCAATCGTGAGGCCGGCATCGGCGGCGGCGGGCGGCGGCGAGGTGCAGGAGAGTGGTGG- GAGGAGCTTGGCG GCGGTGGAGAAGGAGCACATGTTCGACAAGGTGGTGACGCCGAGCGACGTGGGGAAGCTGAACCGGCTGGTGAT- CCCGAAGCAGCAC GCGGAGAAGTACTTCCCGCTGGACGCGGCGTCCAACGAGAAGGGGCTCCTGCTCAGCTTCGAGGACCGCACGGG- GAAGCCATGGCGG TTCCGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTGATGACCAAGGGGTGGAGCCGCTTCGTCAAGGAGAA- GCGACTCGACGCC GGGGACACCGTCTCCTTCGGCCGCGGCGTCGGCGAGGCCGCGCGCGGGAGGCTCTTCATCGACTGGCGCCGCCG- CCCCGACGTCGTC GCCGCGCTCCAGCCGCCCACGCACCGCTTCGCCCACCACCTCCCTTCCTCCATCCCCTTCGCTCCCTGGGCGCA- CCACCACGGACAC GGAGCCGCCGCCGCCGCCGCCGCCGCCGCCGGCGCCAGGTTTCTCCTGCCTCCCTCCTCGACTCCCATCTACGA- CCACCACCGCCGA CACGCCCACGCCGTCGGGTACGACGCGTACGCCGCGGCCACCAGCAGGCAGGTGCTGTTCTACCGGCCGTTGCC- GCCGCAGCAGCAG CATCATCCCGCGGTGGTGCTGGAGTCGGTGCCGGTGCGCATGACGGCGGGGCACGCGGAGCCGCCGTCGGCTCC- GTCGAAGCGAGTT CGGCTGTTCGGGGTGAACCTCGACTGCGCGAATTCCGAACAAGACCACGCCGGCGTGGTCGGGAAGACGGCGCC- GCCGCCGCTGCCA TCGCCGCCGTCATCATCGTCATCTTCCTCCGGGAAAGCGAGGTGCTCCTTGAACCTTGACTTGTGA SEQ ID NO: 58 MEFITPIVRPASAAAGGGEVQESGGRSLAAVEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDAASNEKGL- LLSFEDRTGKPWR FRYSYWNSSQSYVMTKGWSRFVKEKRLDAGDTVSFGRGVGEAARGRLFIDWRRRPDVVAALQPPTHRFAHHLPS- SIPFAPWAHHHGH GAAAAAAAAAGARFLLPPSSTPIYDHHRRHAHAVGYDAYAAATSRQVLFYRPLPPQQQHHPAVVLESVPVRMTA- GHAEPPSAPSKRV RLFGVNLDCANSEQDHAGVVGKTAPPPLPSPPSSSSSSSGKARCSLNLDL Os01g0693400 Cover 47% identity 63% CDS SEQ ID NO: 59 ATGGACAGCTCCAGCTGCCTGGTGGATGATACCAACAGCGGCGGCTCGTCCACGGACAAGCTGAGGGCGTTGGC- CGCCGCGGCGGCG GAGACGGCGCCGCTGGAGCGCATGGGGAGCGGGGCGAGCGCGGTGGTGGACGCGGCCGAGCCTGGCGCGGAGGC- GGACTCCGGGTCC GGGGGACGTGTGTGCGGCGGCGGCGGCGGCGGTGCCGGCGGTGCGGGAGGGAAGCTGCCGTCGTCCAAGTTCAA- GGGCGTCGTGCCG CAGCCCAACGGGAGGTGGGGCGCGCAGATCTACGAGCGGCACCAGCGGGTGTGGCTCGGCACGTTCGCCGGGGA- GGACGACGCCGCG CGCGCCTACGACGTCGCCGCGCAGCGCTTCCGCGGCCGCGACGCCGTCACCAACTTCCGCCCGCTCGCCGAGGC- CGACCCGGACGCC GCCGCCGAGCTTCGCTTCCTCGCCACGCGCTCCAAGGCCGAGGTCGTCGACATGCTCCGCAAGCACACCTACTT- CGACGAGCTCGCG CAGAGCAAGCGCACCTTCGCCGCCTCCACGCCGTCGGCCGCGACCACCACCGCCTCCCTCTCCAACGGCCACCT- CTCGTCGCCCCGC TCCCCCTTCGCGCCCGCCGCGGCGCGCGACCACCTGTTCGACAAGACGGTCACCCCGAGCGACGTGGGCAAGCT- GAACAGGCTCGTC ATACCGAAGCAGCACGCCGAGAAGCACTTCCCGCTACAGCTCCCGTCCGCCGGCGGCGAGAGCAAGGGTGTCCT- CCTCAACTTCGAG GACGCCGCCGGCAAGGTGTGGCGGTTCCGGTACTCGTACTGGAACAGCAGCCAGAGCTACGTGCTAACCAAGGG- CTGGAGCCGCTTC GTCAAGGAGAAGGGTCTCCACGCCGGCGACGTCGTCGGCTTCTACCGCTCCGCCGCCAGTGCCGGCGACGACGG- CAAGCTCTTCATC GACTGCAAGTTAGTACGGTCGACCGGCGCCGCCCTCGCGTCGCCCGCTGATCAGCCAGCGCCGTCGCCGGTGAA- GGCCGTCAGGCTC TTCGGCGTGGACCTGCTCACGGCGCCGGCGCCGGTCGAACAGATGGCCGGGTGCAAGAGAGCCAGGGACTTGGC- GGCGACGACGCCT CCACAAGCGGCGGCGTTCAAGAAGCAATGCATAGAGCTGGCACTAGTATAG SEQ ID NO: 49 60MDSSSCLVDDTNSGGSSTDKLRALAAAAAETAPLERMGSGASAVVDAAEPGAEADSGSGGRVCGGGGGGAGG- AGGKLPSSKFKGV VPQPNGRWGAQIYERHQRVWLGTFAGEDDAARAYDVAAQRFRGRDAVTNFRPLAEADPDAAAELRFLATRSKAE- VVDMLRKHTYFDE LAQSKRTFAASTPSAATTTASLSNGHLSSPRSPFAPAAARDHLFDKTVTPSDVGKLNRLVIPKQHAEKHFPLQL- PSAGGESKGVLLN FEDAAGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKGLHAGDVVGFYRSAASAGDDGKLFIDCKLVRSTGAALAS- PADQPAPSPVKAV RLFGVDLLTAPAPVEQMAGCKRARDLAATTPPQAAAFKKQCIELALV Os10g0537100 LOC_Os10g39190 Cover 47% identity 60% CDS SEQ ID NO: 61 ATGGAGTTCACCCCAATTTCGCCGCCGACGAGGGTCGCCGGCGGTGAGGAGGATTCCGAGAGGGGGGCGGCGGC- GTGGGCGGTGGTG GAGAAGGAGCACATGTTTGAGAAGGTCGTGACGCCGAGCGACGTGGGGAAGCTGAACCGATTGGTCATCCCCAA- GCAGCACGCCGAG AGGTACTTCCCGCTCGACGCCGCGGCGGGCGCCGGCGGCGGCGGTGGTGGCGGCGGTGGCGGCGGCGGGGGGAA- GGGGCTGGTGCTG AGCTTCGAGGACAGGACGGGGAAGGCGTGGAGGTTCCGGTACTCGTACTGGAACAGCAGCCAGAGCTACGTGAT- GACCAAAGGGTGG AGCCGCTTCGTCAAGGAGAAGCGCCTCGGCGCCGGCGACACCGTGTCGTTCGGCCGCGGCCTCGGCGACGCCGC- CCGCGGCCGCCTC TTCATCGACTTCCGCCGCCGCCGCCAGGACGCCGGCAGCTTCATGTTCCCGCCGACGGCGGCGCCGCCGTCGCA- CTCGCACCACCAT CATCAGCGACACCACCCGCCGCTCCCGTCCGTGCCCCTTTGCCCGTGGCGAGACTACACCACCGCCTATGGCGG- CGGCTACGGCTAC GGCTACGGCGGCGGCTCCACCCCGGCGTCCAGCCGCCACGTGCTGTTCCTCCGGCCGCAGGTGCCGGCCGCTGT- GGTGCTCAAGTCG GTGCCGGTGCACGTCGCGGCCACCTCGGCGGTGCAGGAGGCGGCGACGACGACAAGGCCGAAGCGTGTCCGGCT- GTTCGGGGTGAAC CTCGACTGCCCGGCGGCCATGGACGACGACGACGACATCGCCGGAGCGGCGAGCCGGACGGCAGCGTCGTCTCT- CCTGCAGCTCCCC TCGCCGTCGTCCTCGACGTCGTCGTCGACGGCGGGGAAGAAGATGTGCTCCTTGGATCTTGGGTTGTGA SEQ ID NO: 62 MEFTPISPPTRVAGGEEDSERGAAAWAVVEKEHMFEKVVTPSDVGKLNRLVIPKQHAERYFPLDAAAGAGGGGG- GGGGGGGGKGLVL SFEDRTGKAWRFRYSYWNSSQSYVMTKGWSRFVKEKRLGAGDTVSFGRGLGDAARGRLFIDFRRRRQDAGSFMF- PPTAAPPSHSHHH HQRHHPPLPSVPLCPWRDYTTAYGGGYGYGYGGGSTPASSRHVLFLRPQVPAAVVLKSVPVHVAATSAVQEAAT- TTRPKRVRLFGVN LDCPAAMDDDDDIAGAASRTAASSLLQLPSPSSSTSSSTAGKKMCSLDLGL Glycine max Loc100795470 Cover 75% identity 53% SEQ ID NO: 63 Msinhysmdlpeptlwwphphhqqqqltlmdpdplrlnlnsddgngndndndenqttttggeqeilddkepmfe- kpltpsdvgklnr lvipkqhaekyfplsgdsggseckglllsfedesgkcwrfrysywnssqsyvltkgwsryvkdkrldagdvvlf- erhrvdaqrlfig wrrrrqsdaalpphavssrksgggdgnsnknegwtrgfysahhpypthhlhhhqpspyqqqhdclhagrgsqgq- nqrmrpvgnnsss sssssrvlrlfgvdmecqpehddsgpstpqcsynsnnmlpstqgtdhshhnfyqqqpsnsnpsphhmmvhhqpy- yy CDS SEQ ID NO: 64 ATGTCCATAAACCACTACTCCATGGACCTTCCCGAACCGACACTCTGGTGGCCACACCCACACCACCAACAACA- ACAACTAACCTTA ATGGATCCTGACCCTCTCCGTCTCAACCTCAATAGCGACGATGGCAATGGCAATGACAACGACAACGACGAAAA- TCAAACAACCACA ACAGGAGGAGAACAAGAAATATTAGACGATAAAGAACCGATGTTCGAGAAGCCCTTAACCCCGAGCGACGTGGG- GAAGCTGAACCGT CTCGTAATCCCGAAGCAGCACGCGGAGAAGTACTTCCCACTGAGTGGTGACTCGGGCGGGAGCGAGTGCAAGGG- GCTGTTACTGAGT TTCGAGGACGAGTCGGGGAAGTGTTGGCGCTTCCGCTACTCGTACTGGAACAGCAGCCAGAGCTACGTGCTCAC- CAAAGGGTGGAGC CGCTACGTCAAGGACAAGCGCCTTGACGCGGGCGACGTCGTTTTGTTCGAGCGTCACCGCGTCGACGCGCAGCG- CCTCTTCATCGGG TGGAGGCGCAGGCGGCAGAGCGATGCCGCCTTGCCGCCTGCGCACGTTAGCAGTAGGAAGAGTGGTGGTGGTGA- TGGGAATAGTAAT AAGAATGAGGGGTGGACCAGAGGGTTCTATTCTGCGCATCATCCTTATCCTACGCATCATCTTCATCATCATCA- GCCCTCGCCATAC CAACAACAACATGACTGTCTTCATGCAGGTAGAGGGTCCCAAGGTCAGAACCAAAGGATGAGACCAGTGGGAAA- CAACAGTTCTAGC TCTAGTTCGAGTTCAAGGGTACTTAGGCTGTTCGGGGTCGACATGGAATGCCAACCCGAACATGATGATTCTGG- TCCCTCCACACCC CAATGCTCCTACAATAGTAACAACATGTTGCCATCAACACAGGGCACAGATCATTCCCATCACAATTTCTACCA- ACAGCAACCTTCT AATTCCAATCCTTCCCCTCATCACATGATGGTACATCACCAACCATACTACTACTAG Loc100818164 Cover 50% identity 73% SEQ ID NO: 65 MSTNHYTMDLPEPTLWWPHPHQQQLTLIDPDPLPLNLNNDDNDNGDDNDNDENQTVTTTTTGGEEEIINNKEPM- FEKPLTPSDVGKL NRLVIPKQHAEKYFPLSGGDSGSSECKGLLLSFEDESGKCWRFRYSYWNSSQSYVLTKGWSRYVKDKRLDAGDV- VLFQRHRADAQRL FIGWRRRRQSDALPPPAHVSSRKSGGDGNSSKNEGDVGVGWTRGFYPAHHPYPTHHHHPSPYHHQQDDSLHAVR- GSQGQNQRTRPVG NSSSSSSSSSRVLRLFGVNMECQPEHDDSGPSTPQCSYNTNNILPSTQGTDIHSHLNFYQQQQTSNSKPPPHHM- MIRHQPYYY SEQ ID NO: 66 ATGTCGACAAACCACTACACCATGGACCTTCCCGAACCAACACTCTGGTGGCCACACCCACACCAACAACAACT- AACCTTAATAGAT CCAGACCCTCTCCCTCTGAACCTCAACAACGACGACAACGACAATGGCGACGACAACGACAACGACGAAAACCA- AACAGTTACAACA ACCACAACAGGAGGAGAAGAAGAAATAATAAACAATAAAGAACCGATGTTCGAGAAGCCGCTAACCCCGAGCGA- CGTGGGGAAGCTG AACCGCCTCGTAATCCCGAAGCAGCACGCTGAGAAGTACTTTCCACTGAGTGGTGGTGACTCGGGCAGTAGCGA- GTGCAAGGGGCTG TTACTGAGTTTCGAGGACGAGTCGGGGAAGTGCTGGCGCTTCCGCTACTCGTACTGGAACAGCAGCCAGAGCTA- CGTGCTCACCAAA GGGTGGAGCCGTTACGTGAAGGACAAGCGCCTCGATGCGGGAGATGTCGTTTTATTCCAGCGCCACCGCGCCGA- CGCGCAGCGCCTC TTCATCGGCTGGAGGCGCAGGCGGCAGAGCGACGCCCTGCCGCCGCCTGCGCACGTTAGCAGCAGGAAGAGTGG- TGGTGATGGGAAT AGTAGTAAGAATGAGGGTGATGTGGGCGTGGGCTGGACCAGAGGGTTCTATCCTGCGCATCATCCTTATCCTAC- GCATCATCATCAT CCCTCGCCATACCATCACCAACAAGATGACTCTCTTCATGCAGTTAGAGGGTCCCAAGGTCAGAACCAAAGGAC- GAGACCAGTGGGA AACAGCAGTTCTAGTTCGAGTTCGAGTTCAAGGGTACTTAGGCTATTCGGGGTCAACATGGAATGCCAACCCGA- ACATGATGATTCT GGACCCTCCACACCCCAATGCTCCTACAATACTAACAACATATTGCCATCCACACAGGGCACAGATATTCATTC- CCATCTCAATTTC

TACCAACAACAACAAACTTCTAATTCCAAGCCTCCCCCTCATCACATGATGATACGTCACCAACCATACTACTA- CTAG Loc100802734 Cover 77% identity 53% SEQ ID NO: 67 MSSINHYSPETTLYWTNDQQQQAAMWLSNSHTPRFNLNDEEEEEEDDVIVSDKATNNLTQEEEKVAMFEKPLTP- SDVGKLNRLVIPK QHAEKHFPLDSSAAKGLLLSFEDESGKCWRFRYSYWNSSQSYVLTKGWSRYVKDKRLHAGDVVLFHRHRSLPQR- FFISCSRRQPNPV PAHVSTTRSSASFYSAHPPYPAHHFPFPYQPHSLHAPGGGSQGQNETTPGGNSSSSGSGRVLRLFGVNMECQPD- NHNDSQNSTPECS YTHLYHHQTSSYSSSSNPHHHMVPQQP SEQ ID NO: 68 ATGTCATCGATAAACCACTATTCACCGGAAACAACACTATACTGGACCAACGACCAACAGCAACAAGCCGCCAT- GTGGCTGAGTAAT TCCCACACCCCGCGTTTCAATCTGAACGACGAGGAGGAGGAGGAGGAAGACGACGTTATCGTTTCGGACAAGGC- TACTAATAACTTG ACGCAAGAGGAGGAGAAGGTAGCCATGTTCGAGAAGCCGTTGACGCCGAGCGACGTCGGGAAGCTGAACCGGCT- CGTGATTCCGAAA CAGCACGCGGAGAAGCACTTCCCTCTCGACTCGTCGGCGGCGAAGGGGCTGTTGCTGAGTTTCGAGGACGAGTC- CGGGAAGTGTTGG CGCTTCCGTTACTCTTATTGGAACAGTAGCCAGAGTTACGTTTTGACCAAAGGATGGAGCCGTTACGTCAAAGA- CAAACGCCTCCAC GCTGGCGACGTCGTTTTGTTCCACAGACACCGCTCCCTCCCTCAACGCTTCTTCATCTCCTGCAGCCGCCGCCA- ACCCAACCCGGTC CCCGCTCACGTTAGCACCACCAGATCCTCCGCTTCCTTCTACTCTGCGCACCCACCTTATCCTGCGCACCACTT- CCCCTTCCCATAC CAACCTCACTCTCTTCATGCACCAGGTGGAGGGTCCCAAGGACAGAACGAAACGACACCGGGAGGGAACAGTAG- TTCAAGTGGCAGT GGCAGGGTGCTGAGGCTCTTTGGTGTGAACATGGAATGCCAACCTGATAATCATAATGATTCCCAGAACTCCAC- ACCAGAATGCTCC TACACCCACTTATACCACCATCAAACCTCTTCTTATTCTTCTTCTTCAAACCCTCACCATCACATGGTACCTCA- ACAACCATAA Loc100781489 Cover 49% identity 64% SEQ ID NO: 69 MELMQQVKGNYSDSREEEEEEEAAAITRESESSRLHQQDTASNFGKKLDLMDLSLGSSKEEEEEGNLQQGGGGV- VHHAHQVVEKEHM FEKVATPSDVGKLNRLVIPKQHAEKYFPLDSSTNEKGLLLNFEDRNGKVWRFRYSYWNSSQSYVMTKGWSRFVK- EKKLDAGDIVSFQ RGLGDLYRHRLYIDWKRRPDHAHAHPPHHHDPLFLPSIRLYSLPPTMPPRYHHDHHFHHHLNYNNLFTFQQHQY- QQLGAATTTHHNN YGYQNSGSGSLYYLRSSMSMGGGDQNLQGRGSNIVPMIIDSVPVNVAHHNNNRHGNGGITSGGTNCSGKRLRLF- GVNMECASSAEDS KELSSGSAAHVTTAASSSSLHHQRLRVPVPVPLEDPLSSSAAAAARFGDHKGASTGTSLLFDLDPSLQYHRH CDS SEQ ID NO: 70 ATGGAGTTGATGCAACAAGTTAAAGGTAATTATTCTGATAGCAGGGAGGAAGAGGAGGAAGAGGAAGCTGCAGC- AATCACAAGGGAA TCAGAAAGCAGCAGGTTACACCAACAAGATACAGCATCCAATTTTGGAAAGAAGCTAGACTTGATGGACTTGTC- ACTAGGGAGCAGC AAGGAAGAGGAAGAGGAAGGGAATTTGCAACAAGGAGGAGGAGGAGTGGTTCATCATGCTCACCAAGTAGTGGA- GAAAGAACACATG TTTGAGAAAGTGGCGACACCGAGCGACGTAGGGAAGCTGAACAGGCTGGTGATACCGAAGCAGCACGCGGAGAA- GTACTTCCCCCTT GACTCCTCAACCAACGAGAAGGGTCTGCTCCTGAATTTCGAGGACAGGAATGGGAAGGTGTGGCGATTCAGGTA- TTCCTATTGGAAC AGCAGCCAGAGCTATGTGATGACAAAAGGGTGGAGCCGCTTTGTTAAGGAGAAGAAGCTGGATGCCGGTGACAT- TGTCTCCTTCCAG CGTGGCCTTGGGGATTTGTATAGACATCGGTTGTATATAGATTGGAAGAGAAGGCCCGATCATGCTCATGCTCA- TCCACCTCATCAT CACGATCCTTTGTTTCTTCCCTCTATCAGATTGTACTCTCTCCCTCCCACCATGCCACCTCGCTACCACCACGA- TCATCACTTTCAC CACCATCTCAATTACAACAACCTCTTCACTTTTCAGCAACACCAGTACCAGCAGCTTGGTGCTGCCACTACCAC- TCATCACAACAAC TATGGTTACCAGAATTCGGGATCTGGTTCACTCTATTACCTAAGGTCCTCTATGTCAATGGGTGGTGGTGATCA- AAACTTGCAAGGG AGAGGGAGCAACATTGTCCCCATGATCATTGATTCTGTGCCGGTTAACGTTGCTCATCACAACAACAATCGCCA- TGGGAATGGGGGC ATCACGAGTGGTGGTACTAATTGTAGTGGAAAACGACTAAGGCTATTTGGGGTGAACATGGAATGCGCTTCTTC- GGCAGAAGATTCC AAAGAATTGTCCTCGGGTTCGGCAGCACACGTGACGACAGCTGCTTCTTCTTCTTCTCTTCATCATCAGCGCTT- GAGGGTGCCAGTG CCAGTGCCACTTGAAGATCCACTTTCGTCGTCAGCAGCAGCAGCAGCAAGGTTTGGGGATCACAAAGGGGCCAG- TACTGGGACTTCG CTGCTGTTTGATTTGGATCCCTCTTTGCAGTATCATCGCCACTGA Loc100776987 Cover 46% identity 62% SEQ ID NO: 71 MDAISCLDESTTTESLSISQAKPSSTIMSSEKASPSPPPPNRLCRVGSGASAVVDSDGGGGGGSTEVESRKLPS- SKYKGVVPQPNGR WGSQIYEKHQRVWLGTFNEEDEAARAYDVAVQRFRGKDAVTNFKPLSGTDDDDGESEFLNSHSKSEIVDMLRKH- TYNDELEQSKRSR GFVRRRGSAAGAGNGNSISGACVMKAREQLFQKAVTPSDVGKLNRLVIPKQHAEKHFPLQSAANGVSATATAAK- GVLLNFEDVGGKV WRFRYSYWNSSQSYVLTKGWSRFVKEKNLKAGDTVCFQRSTGPDRQLYIDWKTRNVVNEVALFGPVVEPIQMVR- LFGVNILKLPGSD SIANNNNASGCCNGKRREMELFSLECSKKPKIIGAL CDS SEQ ID NO: 72 ATGGATGCAATTAGTTGCCTGGATGAGAGCACCACCACCGAGTCACTCTCCATAAGTCAGGCGAAGCCTTCTTC- GACGATTATGTCG TCCGAGAAGGCTTCTCCTTCCCCGCCGCCGCCGAACAGGCTGTGCCGCGTCGGTAGCGGTGCTAGCGCAGTCGT- GGATTCCGACGGC GGCGGCGGGGGTGGCAGCACCGAGGTGGAGTCGCGGAAGCTCCCCTCGTCCAAGTATAAGGGCGTCGTGCCCCA- GCCCAACGGCCGC TGGGGCTCGCAGATTTACGAGAAGCACCAGCGCGTGTGGCTGGGAACGTTCAACGAGGAAGACGAGGCGGCGCG- TGCGTACGACGTC GCCGTGCAGCGATTCCGCGGCAAGGACGCCGTCACAAACTTCAAGCCGCTCTCCGGCACCGACGACGACGACGG- GGAATCGGAGTTT CTCAACTCGCATTCGAAATCCGAGATCGTCGACATGCTGCGTAAGCATACGTACAATGACGAGCTGGAACAAAG- CAAGCGCAGCCGC GGCTTCGTACGTCGGCGCGGCTCCGCCGCCGGCGCCGGAAACGGAAACTCAATCTCCGGCGCGTGTGTTATGAA- GGCGCGTGAGCAG CTATTCCAGAAGGCCGTTACGCCGAGCGACGTTGGGAAACTGAACCGTTTGGTGATACCGAAGCAGCACGCGGA- GAAGCACTTTCCT TTACAGAGCGCTGCTAACGGCGTTAGCGCGACGGCGACGGCGGCGAAGGGCGTTTTGTTGAACTTCGAAGACGT- TGGAGGGAAAGTG TGGCGGTTTCGTTACTCGTATTGGAACAGTAGCCAGAGTTACGTCTTGACCAAAGGTTGGAGCCGGTTCGTTAA- GGAGAAGAATCTG AAAGCCGGTGACACGGTTTGTTTTCAACGGTCCACTGGACCGGACAGGCAGCTTTACATCGATTGGAAGACGAG- GAATGTTGTTAAC GAGGTCGCGTTGTTCGGACCGGTTGTCGAACCGATCCAGATGGTTCGGCTCTTTGGTGTTAACATTTTGAAACT- ACCCGGTTCAGAT TCTATCGCCAATAACAATAATGCAAGTGGGTGCTGCAATGGCAAGAGAAGAGAAATGGAACTCTTTTCATTAGA- GTGTAGCAAGAAA CCTAAGATTATTGGTGCTTTGTAG Loc100778733 Cover 44% identity 64% SEQ ID NO: 73 MELMQEVKGYSDGREEEEEEEEAAEEIITREESSRLLHQHQEAAGSNFIINNNHHHHQHHHHHTTKQLDFMDLS- LGSSKDEGNLQGS SSSVYAHHHHAASASSSANGNNNNSSSSNLQQQQQQPAEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDS- SANEKGLLLNFED RNGKLWRFRYSYWNSSQSYVMTKGWSRFVKEKKLDAGDMVSFQRGVGELYRHRLYIDWWRRPDHHHHHHHGPDH- STTLFTPFLIPNQ PHHLMSIRWGATGRLYSLPSPTPPRHHENLNYNNNAMYHPFHHHGAGSGINATTHHYNNYHEMSSTTTSGSAGS- VFYHRSTPPISMP LADHQTLNTRQQQQQQQQQEGAGNVSLSPMIIDSVPVAHHLHHQQHHGGKSSGPSSTSTSPSTAGKRLRLFGVN- MECASSTSEDPKC FSLLSSSSMANSNSQPPLQLLREDTLSSSSARFGDQRGVGEPSMLFDLDPSLQYRQ SEQ ID NO: 74 ATGGAGTTGATGCAAGAAGTGAAAGGGTATTCTGATGGCAGAGAGGAGGAGGAGGAGGAAGAGGAAGCAGCAGA- AGAAATCATCACA AGAGAAGAAAGCAGCAGGTTGTTACACCAGCACCAGGAGGCAGCAGGTTCCAATTTCATCATCAACAATAATCA- TCATCATCATCAA CATCACCACCACCACACAACAAAGCAGCTAGACTTCATGGACTTGTCACTTGGTAGCAGCAAGGATGAAGGGAA- TTTGCAAGGATCA TCTTCTTCTGTCTATGCTCATCATCATCATGCAGCAAGTGCTAGTTCTTCTGCCAATGGTAACAACAACAACAG- CAGCAGCAGCAAC TTGCAGCAACAGCAGCAGCAGCCTGCTGAGAAGGAGCACATGTTTGATAAAGTAGTGACACCAAGTGATGTGGG- GAAGCTGAACCGG TTGGTGATACCAAAGCAGCATGCTGAGAAGTATTTCCCTCTTGATTCCTCAGCCAATGAGAAGGGTCTGTTGCT- GAATTTTGAGGAC AGGAATGGTAAGTTGTGGAGGTTCAGGTACTCCTATTGGAACAGCAGCCAGAGCTATGTGATGACCAAAGGTTG- GAGCCGTTTTGTT AAGGAGAAGAAGCTTGATGCTGGTGACATGGTGTCCTTCCAGCGTGGTGTTGGGGAGTTGTATAGGCATAGGTT- GTACATAGATTGG TGGAGAAGGCCTGATCATCATCACCATCACCATCATGGCCCTGACCATTCAACCACACTCTTCACACCTTTCTT- AATTCCCAATCAG CCTCATCACTTAATGTCCATCAGATGGGGTGCCACTGGCAGATTGTACTCCCTCCCTTCCCCAACCCCACCACG- CCACCATGAACAC CTCAATTACAACAATAACGCCATGTATCATCCCTTTCATCACCATGGTGCTGGAAGTGGAATTAATGCTACTAC- TCATCACTACAAC AACTATCATGAGATGAGTAGTACTACTACTTCAGGATCTGCAGGCTCAGTCTTTTACCACAGGTCAACACCCCC- AATATCAATGCCA TTGGCTGACCACCAAACCTTGAACACAAGGCAGCAGCAACAACAACAACAACAACAAGAGGGAGCTGGCAATGT- TTCTCTTTCCCCT ATGATCATTGATTCTGTTCCAGTTGCTCACCACCTCCATCATCAACAACACCATGGTGGCAAGAGTAGTGGTCC- TAGTAGTACTAGT ACTAGTCCTAGCACTGCAGGGAAAAGACTAAGGCTATTTGGGGTCAACATGGAATGTGCTTCTTCAACATCAGA- AGACCCCAAATGC TTCAGCTTGTTGTCCTCATCTTCAATGGCTAATTCCAATTCACAACCACCACTTCAGCTTTTGAGGGAAGATAC- ACTTTCGTCATCA TCGGCAAGGTTTGGGGATCAGAGAGGAGTAGGGGAACCTTCAATGCTTTTTGATCTGGACCCTTCTTTGCAATA- CCGGCAGTGA Loc732601 Cover 44% identity 62% SEQ ID NO: 75 MDGGCVTDETTTSSDSLSVPPPSRVGSVASAVVDPDGCCVSGEAESRKLPSSKYKGVVPQPNGRWGAQIYEKHQ- RVWLGTFNEEDEA ARAYDIAALRFRGPDAVTNFKPPAASDDAESEFLNSHSKFEIVDMLRKHTYDDELQQSTRGGRRRLDADTASSG- VFDAKAREQLFEK TVTPSDVGKLNRLVIPKQHAEKHFPLSGSGDESSPCVAGASAAKGMLLNFEDVGGKVWRFRYSYWNSSQSYVLT- KGWSRFVKEKNLR AGDAVQFFKSTGPDRQLYIDCKARSGEVNNNAGGLFVPIGPVVEPVQMVRLFGVNLLKLPVPGSDGVGKRKEME- LFAFECCKKLKVI GAL CDS SEQ ID NO: 76 ATGGATGGAGGCTGTGTCACAGACGAAACCACCACATCCAGCGACTCTCTTTCCGTTCCGCCGCCCAGCCGCGT- CGGCAGCGTTGCA AGCGCCGTCGTCGACCCCGACGGTTGTTGCGTTTCCGGCGAGGCCGAATCCCGGAAACTCCCTTCGTCGAAATA- CAAAGGCGTGGTG CCGCAACCGAACGGTCGCTGGGGAGCTCAGATTTACGAGAAGCACCAGCGCGTGTGGCTCGGCACTTTCAACGA- GGAAGACGAAGCC GCCAGAGCCTACGACATCGCCGCGCTGCGCTTCCGCGGCCCCGACGCCGTCACCAACTTCAAGCCTCCCGCCGC- CTCCGACGACGCC GAGTCCGAGTTCCTCAACTCGCATTCCAAGTTCGAGATCGTCGACATGCTCCGCAAGCACACCTACGACGACGA- GCTCCAGCAGAGC ACGCGCGGTGGTAGGCGCCGCCTCGACGCTGACACCGCGTCGAGCGGTGTGTTCGACGCGAAAGCGCGTGAGCA- GCTGTTCGAGAAA ACGGTTACGCCGAGCGACGTCGGGAAGCTGAATCGATTAGTGATACCGAAGCAGCACGCGGAGAAGCACTTTCC- GTTAAGCGGATCC GGCGACGAAAGCTCGCCGTGCGTGGCGGGGGCTTCGGCGGCGAAGGGAATGTTGTTGAACTTTGAGGACGTTGG- AGGGAAAGTGTGG CGGTTTCGTTACTCTTATTGGAACAGTAGCCAGAGCTACGTGCTTACCAAAGGATGGAGCCGGTTCGTTAAGGA- GAAGAATCTTCGA GCCGGTGACGCGGTTCAGTTCTTCAAGTCGACCGGACCGGACCGGCAGCTATATATAGACTGCAAGGCGAGGAG- TGGTGAGGTTAAC AATAATGCTGGCGGTTTGTTTGTTCCGATTGGACCGGTCGTTGAGCCGGTTCAGATGGTTCGGCTTTTCGGGGT- CAACCTTTTGAAA CTACCCGTACCCGGTTCGGATGGTGTAGGGAAGAGAAAAGAGATGGAACTGTTTGCATTTGAATGTTGCAAGAA- GTTAAAAGTAATT GGAGCTTTGTAA Loc100801107 Cover 44% identity 61% SEQ ID NO: 77 MDAISCMDESTTTESLSISLSPTSSSEKAKPSSMITSSEKVSLSPPPSNRLCRVGSGASAVVDPDGGGSGAEVE- SRKLPSSKYKGVV PQPNGRWGAQIYEKHQRVWLGTFNEEDEAARAYDIAAQRFRGKDAVTNFKPLAGADDDDGESEFLNSHSKPEIV- DMLRKHTYNDELE QSKRSRGVVRRRGSAAAGTANSISGACFTKAREQLFEKAVTPSDVGKLNRLVIPKQHAEKHFPLQSSNGVSATT- IAAVTATPTAAKG VLLNFEDVGGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKNLKAGDTVCFHRSTGPDKQLYIDWKTRNVVNNEVA- LFGPVGPVVEPIQ MVRLFGVNILKLPGSDTIVGNNNNASGCCNGKRREMELFSLECSKKPKIIGAL CDS SEQ ID NO: 78 ATGGATGCAATTAGTTGCATGGATGAGAGCACCACCACTGAGTCACTCTCTATAAGTCTTTCTCCGACGTCATC- GTCGGAGAAAGCG AAGCCTTCTTCGATGATTACATCGTCGGAGAAGGTTTCTCTGTCCCCGCCGCCGTCAAACAGACTATGCCGTGT- TGGAAGCGGCGCG AGCGCAGTCGTGGATCCTGATGGCGGCGGCAGCGGCGCTGAGGTAGAGTCGCGGAAACTCCCCTCGTCGAAGTA- CAAGGGCGTGGTG CCCCAGCCCAACGGCCGCTGGGGTGCGCAGATTTACGAGAAGCACCAGCGCGTGTGGCTTGGAACGTTCAACGA- GGAAGACGAGGCG GCGCGTGCGTACGACATCGCCGCGCAGCGGTTCCGCGGCAAGGACGCCGTCACGAACTTCAAGCCGCTCGCCGG- CGCCGACGACGAC GACGGAGAATCGGAGTTTCTCAACTCGCATTCCAAACCCGAGATCGTCGACATGCTGCGAAAGCACACGTACAA- TGACGAGCTGGAG CAGAGCAAGCGCAGCCGCGGCGTCGTCCGGCGGCGAGGCTCCGCCGCCGCCGGCACCGCAAACTCAATTTCCGG- CGCGTGCTTTACT AAGGCACGTGAGCAGCTATTCGAGAAGGCTGTTACGCCGAGCGACGTTGGGAAATTGAACCGTTTGGTGATACC- GAAGCAGCACGCG GAGAAGCACTTTCCGTTACAGAGCTCTAACGGCGTTAGCGCGACGACGATAGCGGCGGTGACGGCGACGCCGAC- GGCGGCGAAGGGC

GTTTTGTTGAACTTCGAAGACGTTGGAGGGAAAGTGTGGCGGTTTCGTTACTCGTATTGGAACAGTAGCCAGAG- TTACGTCTTAACC AAAGGTTGGAGCCGGTTCGTTAAGGAGAAGAATCTGAAAGCTGGTGACACGGTTTGTTTTCACCGGTCCACTGG- ACCGGACAAGCAG CTTTACATCGATTGGAAGACGAGGAATGTTGTTAACAACGAGGTCGCGTTGTTCGGACCGGTCGGACCGGTTGT- CGAACCGATCCAG ATGGTTCGGCTCTTTGGGGTTAACATTTTGAAACTACCCGGTTCAGATACTATTGTTGGCAATAACAATAATGC- AAGTGGGTGCTGC AATGGCAAGAGAAGAGAAATGGAACTGTTCTCGTTAGAGTGTAGCAAGAAACCTAAGATTATTGGTGCTTTGTA- A Loc100789009 Cover 44% identity 62% SEQ ID NO: 79 MDGGSVTDETTTTSNSLSVPANLSPPPLSLVGSGATAVVYPDGCCVSGEAESRKLPSSKYKGVVPQPNGRWGAQ- IYEKHQRVWLGTF NEEDEAARAYDIAAHRFRGRDAVTNFKPLAGADDAEAEFLSTHSKSEIVDMLRKHTYDNELQQSTRGGRRRRDA- ETASSGAFDAKAR EQLFEKTVTQSDVGKLNRLVIPKQHAEKHFPLSGSGGGALPCMAAAAGAKGMLLNFEDVGGKVWRFRYSYWNSS- QSYVLTKGWSRFV KEKNLRAGDAVQFFKSTGLDRQLYIDCKARSGKVNNNAAGLFIPVGPVVEPVQMVRLFGVDLLKLPVPGSDGIG- VGCDGKRKEMELF AFECSKKLKVIGAL SEQ ID NO: 80 ATGGATGGAGGCAGTGTCACAGACGAAACCACCACAACCAGCAACTCTCTTTCGGTTCCGGCGAATCTATCTCC- GCCGCCTCTCAGC CTTGTCGGCAGCGGCGCAACCGCCGTCGTCTACCCCGACGGTTGTTGCGTCTCCGGCGAAGCCGAATCCCGGAA- ACTCCCGTCCTCG AAATACAAAGGCGTGGTGCCGCAACCGAACGGTCGTTGGGGAGCTCAGATTTACGAGAAGCACCAGCGCGTGTG- GCTCGGCACCTTC AACGAGGAAGACGAAGCCGCCAGAGCCTACGACATCGCCGCGCATCGCTTCCGCGGCCGCGACGCCGTCACTAA- CTTCAAGCCTCTC GCCGGCGCCGACGACGCCGAAGCCGAGTTCCTCAGCACGCATTCCAAGTCCGAGATCGTCGACATGCTCCGCAA- GCACACCTACGAC AACGAGCTCCAGCAGAGCACCCGCGGCGGCAGGCGCCGCCGGGACGCCGAAACCGCGTCGAGCGGCGCGTTCGA- CGCGAAGGCGCGT GAGCAGCTGTTCGAGAAAACCGTTACGCAGAGCGACGTCGGGAAGCTGAACCGATTAGTGATACCAAAGCAGCA- CGCGGAGAAGCAC TTTCCGTTAAGCGGATCCGGCGGCGGAGCCTTGCCGTGCATGGCGGCGGCTGCGGGGGCGAAGGGAATGTTGCT- GAACTTTGAGGAC GTTGGAGGGAAAGTGTGGCGGTTCCGTTACTCGTATTGGAACAGTAGCCAGAGCTACGTGCTTACCAAAGGATG- GAGCCGGTTCGTT AAGGAGAAGAATCTTCGAGCTGGTGACGCGGTTCAGTTCTTCAAGTCGACCGGACTGGACCGGCAACTATATAT- AGACTGCAAGGCG AGGAGTGGTAAGGTTAACAATAATGCTGCCGGTTTGTTTATTCCGGTTGGACCGGTTGTTGAGCCGGTTCAGAT- GGTACGGCTTTTC GGGGTCGACCTTTTGAAACTACCCGTACCCGGTTCGGATGGTATTGGGGTTGGCTGTGACGGGAAGAGAAAAGA- GATGGAGCTGTTT GCATTTGAATGTAGCAAGAAGTTAAAAGTAATTGGAGCTTTGTAA Loc102660503 Cover 36% identity 57% SEQ ID NO: 81 migvekvticmrievntegrralmdcwqisgvhessdcseikfafdavvkrarheennaaaqkfkgvvsqqngn- wgaqiyahqqriw lgtfksereaamaydsasiklrsgechrnfpwndqtvqepqfqshysaetvlnmirdgtypskfatflktrqtq- kgvakhiglkgdd eeqfcctqlfqkeltpsdvgklnrlvipkkhavsyfpyvggsadesgsvdveavfydklmrlwkfrycywkssq- syvftrgwnrfvk dkklkakdviafftwgksggegeafalidviynnnaeedskgdtkqvlgnqlqlagseegededanigkdfnaq- kglrlfgvcit CDS SEQ ID NO: 82 atgattggagttgagaaagtgacaatttgtatgagaatagaggtgaatactgaaaagggaagaagggctttaat- ggactgttggcaa atatcaggagttcatgaaagttcagattgtagcgaaatcaaatttgcattcgacgcagtagtaaaacgcgcgag- gcatgaagagaat aatgcagcagcacagaagttcaaaggcgttgtgtctcaacaaaatgggaactggggtgcacagatatatgcaca- ccagcagagaatc tggttggggaccttcaaatctgaaagagaggctgcaatggcttatgacagcgccagcataaaacttagaagcgg- agagtgccacaga aactttccatggaacgaccaaacagttcaagagcctcagttccaaagccattacagcgcagaaacagtgctaaa- catgattagagat ggcacctatccatcaaaatttgctacatttctcaaaactcgtcaaacccaaaaaggcgttgcgaaacacatagg- tctgaagggtgat gacgaggaacagttttgttgcacccaactttttcagaaggaattaacaccaagtgatgtgggcaagctcaacag- gcttgtcatccca aagaagcatgcagttagctattttccttacgttggtggcagtgctgatgagagtggtagtgttgacgtggaggc- tgtgttttatgac aaactcatgcgattgtggaagttccgatactgctattggaagagcagccaaagttacgtgttcaccagaggctg- gaatcggtttgtg aaggataagaagttgaaggctaaagatgtcattgcgttttttacgtggggaaaaagtggaggagagggagaagc- ttttgcattgatc gatgtaatttataataataatgcagaagaagacagcaagggagacaccaaacaagttttgggaaaccaattaca- attagctggcagt gaagaaggtgaagatgaagatgcaaacattggaaaggatttcaatgcacaaaagggtctgaggctctttggtgt- gtgtatcacctaa Hordeum vulgare MLOC_66387 Cover 47% identity 64% SEQ ID NO: 83 MEFTATSSRFSKGEEEVEEEQEEASMREIPFMTPAAATCAAAPPSASASASTPASASGSSPPFRSGDDAGASGS- GAGDGSRSNVAEA VEKEHMFDKVVTPSDVGKLNRLVIPKQYAEKYFPLDSAANEKGLLLNFEDSAGKPWRFRYSYWNSSQSYVMTKG- WSRFVKEKRLDAG DTVSFSRGAGEAARHRLFIDWKRRADTRDPLRLPRLPLPMPLTSHYSPWGLGAGARGFFMPPSPPATLYEHRLR- QGFDFRGMNPSYP TMGRQVILFGSAARMPPHGPAPLLVPRPPPPLHFTVQQQGSDAGGSVTAGSPVVLDSVPVIESPTTATKKRVRL- FGVNLDNPQHPGD GGGESSNYGSALPLQMPASAWRPRDHTLRLLEFPSHGAEASSPSSSSSSKREAHSGLDLDL SEQ ID NO: 84 ATGGAGTTTACTGCGACAAGCAGTAGGTTTTCTAAAGGAGAGGAGGAGGTGGAGGAGGAGCAGGAGGAGGCGTC- GATGCGCGAGATC CCTTTCATGACGCCCGCGGCCGCCACCTGCGCCGCGGCGCCGCCTTCTGCTTCTGCGTCGGCCTCGACACCCGC- GTCAGCGTCTGGA AGTAGCCCTCCCTTTCGATCTGGGGATGACGCCGGAGCGTCGGGGAGCGGGGCCGGCGACGGCAGCCGCAGCAA- CGTGGCGGAGGCC GTGGAGAAGGAGCACATGTTCGACAAAGTGGTGACGCCGAGCGACGTGGGGAAGCTTAACCGGCTGGTCATCCC- CAAGCAGTACGCC GAGAAGTACTTCCCGCTGGACTCGGCGGCCAACGAGAAGGGCCTTCTGCTCAACTTCGAGGACAGCGCCGGGAA- GCCATGGCGCTTC CGCTATTCCTACTGGAACAGCAGCCAGAGCTACGTCATGACCAAAGGCTGGAGCCGCTTCGTCAAGGAGAAGCG- CCTCGACGCTGGG GACACCGTCTCCTTCTCCCGCGGCGCCGGTGAGGCCGCGCGCCACCGCCTCTTCATCGACTGGAAGCGCCGAGC- CGACACCAGAGAC CCGCTCCGCTTGCCCCGCCTCCCGCTCCCGATGCCGCTGACGTCGCACTACAGCCCGTGGGGCCTCGGCGCCGG- CGCCAGAGGATTC TTCATGCCTCCCTCGCCGCCAGCCACGCTCTACGAGCACCGTCTCCGTCAAGGCTTCGACTTCCGCGGCATGAA- CCCCAGTTACCCC ACAATGGGGAGACAGGTCATCCTTTTCGGCTCGGCCGCCAGGATGCCTCCGCACGGACCAGCACCACTCCTCGT- GCCGCGCCCGCCG CCGCCGCTGCACTTCACGGTGCAGCAACAAGGCAGCGACGCCGGCGGAAGTGTAACCGCAGGATCCCCAGTGGT- GCTCGACTCAGTG CCGGTAATCGAAAGCCCCACGACGGCAACGAAGAAGCGCGTGCGCTTGTTCGGCGTGAACTTGGACAACCCGCA- GCATCCCGGTGAT GGCGGGGGCGAATCGAGCAATTATGGCAGTGCACTGCCATTGCAGATGCCCGCATCAGCATGGCGGCCAAGGGA- CCATACGCTGAGG CTGCTCGAATTCCCCTCGCACGGTGCCGAGGCGTCGTCTCCATCGTCGTCGTCGTCTTCCAAGAGGGAGGCGCA- TTCGGGCTTGGAT CTCGATCTGTGA MLOC44012 Cover 55% identity 63% SEQ ID NO: 85 MLRKHTYFDELAQSKRAFAASAALSAPTTSGDAGGSASPPSPAAVREHLFDKTVTPSDVGKLNRLVIPKQNAEK- HFPLQLPAGGGES KGLLLNFEDDAGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKGLGAGDVVGFYRSAAGRTGEDSKFFIDCRLRPN- TNTAAEADPVDQS SAPVQKAVRLFGVDLLAAPEQGMPGGCKRARDLVKPPPPKVAFKKQCIELALA SEQ ID NO: 86 ATGCTCCGCAAGCACACCTACTTCGACGAGCTCGCCCAGAGCAAGCGCGCCTTCGCCGCGTCGGCCGCGCTCTC- CGCGCCCACCACC TCGGGCGACGCCGGCGGCAGCGCCTCGCCGCCCTCCCCGGCCGCCGTGCGCGAGCACCTCTTCGACAAGACCGT- CACGCCCAGCGAC GTCGGCAAGCTGAACAGGCTGGTGATACCGAAGCAGAACGCCGAGAAGCACTTCCCGCTGCAGCTCCCGGCCGG- CGGCGGCGAGAGC AAGGGCCTGCTCCTCAACTTCGAGGACGATGCGGGCAAGGTGTGGCGGTTCCGCTACTCGTACTGGAACAGCAG- CCAGAGCTACGTC CTCACCAAGGGCTGGAGCCGCTTCGTGAAGGAGAAGGGCCTCGGCGCCGGAGACGTCGTCGGGTTCTACCGCTC- CGCCGCCGGGAGG ACCGGCGAAGACAGCAAGTTCTTCATTGACTGCAGGCTGCGGCCGAACACCAACACCGCCGCCGAAGCAGACCC- CGTGGACCAGTCG TCGGCGCCCGTGCAGAAGGCCGTGAGACTCTTCGGCGTCGATCTTCTCGCGGCGCCGGAGCAGGGCATGCCGGG- CGGGTGCAAGAGG GCCAGAGACTTGGTGAAGCCGCCGCCTCCGAAAGTGGCGTTCAAGAAGCAATGCATAGAGCTGGCGCTAGCGTA- G MLOC_57250 Cover 50% identity 57% SEQ ID NO: 87 MYCSRGRIDPAEEGQVMGGLGVRDASWALFKVLEQSDVQVGQNRLLLTKEAVWGGPIPKLFPELEELRGDGLNA- ENRVAVKILDADG CEGDANFRYLNSSKAYRVMGPQWSRLVKETGMCKGDRLDLYAATATAASSCSGARAAVAPAIPPGAIVKAAGF CDS SEQ ID NO: 88 ATGTATTGTTCCCGCGGCCGCATCGATCCCGCGGAAGAAGGGCAGGTGATGGGCGGCCTCGGCGTGCGCGACGC- CAGCTGGGCGCTG TTCAAGGTGTTGGAGCAGTCCGACGTCCAGGTGGGGCAGAACCGGCTGCTCCTCACCAAGGAGGCGGTGTGGGG- CGGCCCTATCCCC AAGCTTTTCCCGGAGCTGGAGGAGCTCCGCGGCGACGGCCTCAACGCCGAGAACAGGGTCGCGGTCAAGATCCT- CGACGCCGACGGC TGCGAGGGGGACGCCAACTTCCGCTACCTCAACTCCAGCAAGGCGTACCGGGTCATGGGGCCTCAGTGGAGCCG- GCTCGTGAAGGAG ACCGGCATGTGCAAGGGAGACCGCCTCGATCTGTACGCGGCAACGGCGACCGCTGCCTCTTCGTGTTCTGGAGC- CAGGGCGGCTGTG GCGCCGGCGATACCTCCCGGAGCAATCGTGAAGGCAGCCGGGTTCTAA MLOC_38822 Cover 47% identity 56% SEQ ID NO: 89 MLRKHIYPDELAQHKRAFFFAAASSPTSSSSPLASPAPSAAAARREHLFDKTVTPSDVGKLNRLVIPKQHAEKH- FPLQLPSASAAVP GECKGVLLNFDDATGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKGLHAGDAVEFYRAASGNNQLFIDCKLRSKS- TTTTTSVNSEAAP SPAPVTRTVRLFGVDLLIAPAARHAHEHEDYGMAKTNKRTMEASVAAPTPAHAVWKKRCVDFALTYRLATTPQC- PRSRDQLEGVQAA GSTFAL CDS SEQ ID NO: 90 ATGCTGCGCAAGCACATCTATCCCGACGAGCTCGCGCAGCACAAGCGCGCCTTCTTCTTCGCCGCGGCGTCGTC- CCCTACGTCGTCG TCGTCACCTCTCGCCTCGCCGGCTCCTTCAGCCGCGGCGGCGCGGCGCGAGCACCTGTTCGACAAGACGGTCAC- GCCCAGCGACGTG GGGAAGCTGAACCGGCTGGTGATCCCCAAGCAGCACGCCGAGAAGCACTTCCCGCTGCAGCTCCCTTCTGCCAG- CGCCGCCGTGCCA GGCGAGTGCAAGGGCGTGCTGCTCAACTTCGATGACGCGACCGGCAAGGTGTGGAGGTTCCGGTACTCCTACTG- GAACAGCAGCCAG AGCTACGTGCTCACCAAGGGGTGGAGCCGCTTCGTGAAGGAGAAGGGCCTTCACGCCGGCGACGCCGTCGAGTT- CTACCGCGCCGCC TCCGGCAACAACCAGCTCTTCATCGACTGCAAGCTCCGGTCCAAGAGCACCACGACGACGACCTCCGTCAACTC- GGAGGCCGCCCCA TCGCCGGCACCCGTGACGAGGACAGTGCGACTCTTCGGGGTCGACCTTCTCATCGCGCCGGCGGCGAGGCACGC- GCATGAGCACGAG GACTACGGCATGGCCAAGACAAACAAGAGAACCATGGAGGCCAGCGTAGCGGCGCCTACTCCGGCGCACGCGGT- GTGGAAGAAGCGG TGCGTAGACTTCGCGCTGACCTACCGACTTGCCACCACCCCACAGTGCCCGAGGTCAAGAGATCAACTAGAAGG- AGTACAAGCAGCT GGGAGTACATTTGCTCTATAG MLOC_7940 Cover 49% identity 52% SEQ ID NO: 91 MGVEILSSTGEHSSQYSSGAASTATTESGVGGRPPTAPSLPVSIADESATSRSASAQSTSSRFKGVVPQPNGRW- GAQIYERHARVWL GTFPDEDSAARAYDVAALRYRGREAATNFPCAAAEAELAFLAAHSKAEIVDMLRKHTYTDELRQGLRRGRGMGA- RAQPTPSWAREPL FEKAVTPSDVGKLNRLVVPKQHAEKHFPLKRTPETTTTTGKGVLLNFEDGEGKVWRFRYSYWNSSQSYVLTKGW- SRFVREKGLGAGD SIVFSCSAYGQEKQFFIDCKKNKTMTSCPADDRGAATASPPVSEPTKGEQVRVVRLFGVDIAGEKRGRAAPVEQ- ELFKRQCVAHSQH SPALGAFVL CDS SEQ ID NO: 92 ATGGGGGTGGAGATCCTGAGCTCAACGGGGGAACACTCCTCCCAGTACTCTTCCGGAGCCGCGTCCACGGCGAC- GACGGAGTCAGGC GTGGGCGGACGGCCGCCGACTGCGCCGAGCCTACCTGTTTCCATCGCCGACGAGTCGGCGACCTCGCGGTCGGC- ATCGGCGCAGTCG ACGTCGTCGCGGTTCAAGGGCGTGGTGCCGCAGCCCAACGGGCGGTGGGGCGCCCAGATCTACGAGCGCCACGC- CCGCGTCTGGCTC GGCACGTTCCCGGACGAAGACTCTGCGGCGCGCGCCTACGACGTGGCCGCGCTCCGGTACCGGGGCCGCGAGGC- CGCCACCAACTTC CCGTGCGCGGCCGCCGAGGCGGAGCTCGCCTTCCTGGCGGCACACTCCAAGGCCGAGATCGTCGACATGCTCCG- GAAGCACACCTAC ACCGACGAGCTCCGCCAGGGCCTGCGGCGCGGCCGCGGCATGGGGGCGCGCGCGCAGCCGACGCCGTCGTGGGC- GCGGGAGCCCCTT TTCGAGAAGGCCGTGACCCCGAGCGACGTGGGCAAGCTCAACCGCCTCGTTGTGCCGAAGCAGCACGCCGAGAA- GCACTTCCCCCTG AAACGCACGCCGGAGACGACAACGACCACCGGCAAGGGGGTGCTTCTCAACTTCGAGGATGGCGAGGGGAAAGT- GTGGAGGTTCCGG

TACTCGTATTGGAACAGCAGCCAGAGCTACGTGCTCACCAAGGGATGGAGCCGCTTCGTTCGGGAGAAGGGCCT- CGGTGCCGGCGAC TCCATCGTGTTCTCCTGCTCGGCGTACGGTCAGGAGAAGCAGTTCTTCATCGACTGCAAGAAGAACAAGACGAT- GACGAGCTGCCCC GCCGATGACCGCGGCGCCGCAACAGCGTCGCCGCCAGTGTCAGAGCCAACAAAAGGAGAACAAGTCCGTGTTGT- GAGGCTGTTCGGC GTCGACATCGCCGGAGAGAAGAGGGGGCGAGCGGCGCCGGTGGAGCAGGAGTTGTTCAAGAGGCAATGCGTGGC- ACACAGCCAGCAC TCTCCAGCCCTAGGTGCCTTCGTCTTATAG MLOC_56567 Cover 42% identity 59% SEQ ID NO: 93 MGVEILSSMVEHSFQYSSGASSATAESGAVGTPPRHLSLPVAIADESLTSRSASSRFKGVVPQPNGRWGAQIYE- RHARVWLGTFPDQ DSAARAYDVASLRYRGGDAAFNFPCVVVEAELAFLAAHSKAEIVDMLRKQTYADELRQGLRRGRGMGVRAQPMP- SWARVPLFEKAVT PSDVGKLNRLVVPKQHAEKHFPLKRSPETTTTTGNGVLLNFEDGQGKVWRFRYSYWNSSQSYVLTKGWSRFVRE- KGLGAGDSIMFSC SAYGQEKQFFIDCKKNTTVNGGKSASPLQVMEIAKAEQVRVVRLFGVDIAGVKRERAATAEQGPQGWFKRQCMA- HGQHSPALGDFAL SEQ ID NO: 94 ATGGGGGTGGAGATCCTGAGCTCCATGGTGGAGCACTCCTTCCAGTACTCTTCGGGCGCGTCCTCGGCCACCGC- GGAGTCAGGCGCC GTCGGAACACCGCCGAGGCATCTGAGCCTACCTGTCGCCATCGCCGACGAGTCCCTGACCTCACGGTCGGCGTC- GTCTCGGTTCAAG GGCGTGGTGCCGCAGCCCAACGGGCGGTGGGGCGCCCAGATCTACGAGCGCCACGCTCGCGTCTGGCTCGGCAC- GTTCCCAGACCAG GACTCGGCGGCGCGCGCCTACGACGTTGCCTCGCTCAGGTACCGCGGCGGCGACGCCGCCTTCAACTTCCCGTG- CGTGGTGGTGGAG GCGGAGCTCGCCTTCCTGGCGGCGCACTCCAAGGCTGAGATCGTTGACATGCTCCGGAAGCAGACCTACGCCGA- TGAACTCCGCCAG GGACTACGGCGCGGCCGTGGCATGGGGGTGCGCGCGCAGCCGATGCCGTCGTGGGCGCGGGTTCCCCTTTTCGA- GAAGGCCGTGACC CCTAGCGATGTCGGCAAGCTCAATCGCCTGGTGGTGCCGAAGCAGCACGCCGAGAAGCACTTCCCCCTGAAGCG- CAGCCCGGAGACG ACGACCACCACCGGCAACGGCGTACTGCTCAACTTTGAGGACGGCCAGGGAAAAGTGTGGAGGTTCCGGTACTC- ATATTGGAACAGC AGCCAGAGCTACGTGCTCACCAAAGGCTGGAGCCGCTTCGTCCGGGAGAAGGGCCTCGGCGCCGGTGACTCCAT- CATGTTCTCCTGC TCGGCGTACGGGCAGGAGAAGCAGTTCTTCATCGACTGCAAGAAGAACACGACCGTGAACGGAGGCAAATCGGC- GTCGCCGCTGCAG GTGATGGAGATTGCCAAAGCAGAACAAGTCCGCGTCGTTAGACTGTTCGGTGTCGACATCGCCGGGGTGAAGAG- GGAGCGAGCGGCG ACGGCGGAGCAAGGCCCGCAGGGGTGGTTCAAGAGGCAATGCATGGCACACGGCCAGCACTCTCCTGCCCTAGG- TGACTTCGCCTTA TAG MLOC_75135 Cover 43% identity 57% SEQ ID NO: 95 MGMEILSSTVEHCSQYSSSASTATTESGAAGRSTTALSLPVAITDESVTSRSASAQPASSRFKGVVPQPNGRWG- SQIYERHARVWLG TFPDQDSAARAYDVASLRYRGRDAATNFPCAAAEAELAFLTAHSKAEIVDMLRKHTYADELRQGLRRGRGMGAR- AQPTPSWARVPLF EKAVTPSDVGKLNRLVVPKQHAEKHFPLKCTAETTTTTGNGVLLNFEDGEGKVWRFRYSYWNSSQSYVLTKGWS- SFVREKGLGAGDS IVFSSSAYGQEKQLFINCKKNTTMNGGKTALPLPVVETAKGEQDHVVKLFGVDIAGVKRVRAATGELGPPELFK- RQSVAHGCGRMNY ICYSIGTIGPLMLN SEQ ID NO: 96 ATGGGGATGGAAATCCTGAGCTCCACGGTGGAGCACTGCTCCCAGTACTCTTCCAGCGCGTCCACGGCCACAAC- GGAGTCAGGCGCC GCCGGAAGATCGACGACGGCTCTGAGCCTACCAGTTGCCATCACCGACGAGTCCGTTACCTCGCGGTCGGCATC- GGCGCAGCCGGCG TCATCACGGTTCAAGGGCGTGGTGCCGCAGCCCAACGGGCGGTGGGGCTCCCAGATCTACGAGCGCCACGCTCG- CGTCTGGCTCGGC ACCTTCCCGGATCAGGACTCGGCGGCGCGTGCCTACGACGTTGCCTCGCTCAGGTACCGGGGCCGCGATGCCGC- CACCAACTTCCCG TGCGCCGCTGCGGAAGCGGAGCTCGCCTTCCTGACCGCGCACTCCAAGGCCGAGATCGTCGACATGCTCCGGAA- GCACACCTACGCC GACGAACTCCGCCAGGGCCTGCGGCGCGGCCGCGGCATGGGTGCGCGCGCGCAGCCGACGCCGTCGTGGGCGCG- GGTTCCCCTTTTC GAGAAGGCTGTGACCCCTAGCGATGTCGGCAAGCTCAATCGCCTGGTGGTGCCGAAGCAGCACGCCGAGAAGCA- CTTCCCCCTGAAG TGCACCGCAGAGACGACGACCACCACCGGCAACGGCGTGCTGCTAAACTTCGAGGATGGTGAGGGGAAGGTGTG- GAGGTTCCGGTAC TCGTATTGGAACAGTAGCCAGAGCTACGTGCTCACCAAAGGCTGGAGCAGCTTCGTCCGGGAGAAGGGCCTCGG- CGCAGGCGACTCC ATCGTCTTCTCCTCCTCGGCGTACGGGCAGGAGAAGCAGTTATTCATCAACTGCAAAAAGAACACGACTATGAA- CGGCGGCAAAACA GCGTTGCCGCTGCCAGTGGTGGAGACTGCCAAAGGAGAACAAGACCACGTCGTTAAGTTGTTCGGTGTTGACAT- CGCCGGTGTGAAG AGGGTGCGAGCGGCGACGGGGGAGCTAGGCCCGCCGGAGTTGTTCAAGAGACAATCCGTGGCACACGGATGCGG- AAGGATGAACTAC ATTTGCTACTCCATAGGGACAATAGGACCTCTTATGCTCAACTGA MLOC_63261 Cover 49% identity 51% SEQ ID NO: 97 MASSKPTNPEVDNDMECSSPESGAEDAVESSSPVAAPSSRFKGVVPQPNGRWGAQIYEKHSRVWLGTFGDEEAA- ACAYDVAALRFRG RDAVTNHQRLPAAEGAGWSSTSELAFLADHSKAEIVDMLRKHTYDDELRQGLRRGHGRAQPTPAWAREFLFEKA- LTPSDVGKLNRLV VPKQHAEKHFPPTTAAAAGSDGKGLLLNFEDGQGKVWRFRYSYWNSSQSYVLTKGWSRFVQEKGLCAGDTVTFS- RSAYVMNDTDEQL FIDYKQSSKNDEAADVATADENEAGHVAVKLFGVDIGWAGMAGSSGG SEQ ID NO: 98 ATGGCGTCTAGCAAGCCGACAAACCCCGAGGTAGACAATGACATGGAGTGCTCCTCCCCGGAATCGGGTGCCGA- GGACGCCGTGGAG TCGTCGTCGCCGGTGGCAGCGCCATCTTCGCGGTTCAAGGGCGTCGTGCCGCAGCCTAACGGGCGCTGGGGCGC- GCAGATCTACGAG AAGCACTCGCGGGTGTGGCTTGGCACGTTCGGGGACGAGGAAGCCGCCGCGTGCGCCTACGACGTGGCCGCGCT- CCGCTTCCGCGGC CGCGACGCCGTCACCAACCACCAGCGCCTGCCGGCGGCGGAGGGGGCCGGCTGGTCGTCCACGAGCGAGCTCGC- CTTCCTCGCCGAC CACTCCAAGGCCGAGATCGTCGACATGCTCCGGAAGCACACCTACGACGACGAGCTCCGGCAGGGCCTGCGCCG- CGGCCACGGGCGC GCGCAGCCCACGCCGGCGTGGGCGCGAGAGTTCCTCTTCGAGAAGGCCCTGACCCCGAGCGACGTCGGCAAGCT- CAACCGCCTGGTC GTTCCGAAGCAGCACGCCGAGAAGCACTTCCCCCCGACGACGGCGGCGGCCGCCGGAAGCGACGGCAAGGGCTT- GCTGCTCAACTTC GAGGACGGCCAAGGGAAGGTGTGGAGGTTCCGGTACTCATACTGGAACAGCAGCCAGAGCTACGTGCTCACCAA- GGGCTGGAGCCGC TTCGTCCAAGAAAAGGGCCTCTGCGCCGGCGACACCGTGACGTTCTCCCGGTCGGCGTACGTGATGAATGACAC- GGATGAGCAGCTC TTCATCGACTACAAGCAGAGTAGCAAGAACGACGAAGCGGCCGACGTAGCCACTGCCGATGAGAATGAGGCCGG- CCATGTCGCCGTG AAGCTCTTCGGGGTCGACATTGGCTGGGCTGGGATGGCGGGATCATCAGGTGGGTGA MLOC_64708 Cover 49% identity 51% SEQ ID NO: 99 MLFDSSVSASLGTMRPLVKKLDMLLAPARGYSTLCKRIKEVMHLLKHDVEEISSYLDELTEVEDPPPMAKCWMN- EARDLSYDMEDYI DSLLFVPPGHFIKKKKKKKKKGKKKMVIKKRLKWCKQIVFTKQVSDHGIKTSKIIHVNVPRLPNKPKVAKIILQ- FRIYVQEAIERYD KYRLHHCSTLRRRLLSTGSMLSVPIPYEEAAQIVTDGRMNEFISSLAANNAADQQQLKVVSVLGSGCLGKTTLA- NVLYDRIGMQFEC RAFIRVSKKPDMKRLFRDLLSQFHQKQPLPTSCNELGISDNIIKHLQDKRYLIVIDDLWDLSVWDIIKYAFPKG- NHGSRIIITTQIE DVALTCCCDHSEHVFEMKPLNIGHSRELFFNRLFGSESDCLEEFKRVSNEIVDICGGLPLATINIASHLANQET- EVSLDLLTDTRDL LRSCLWSNSTSERTKQVLNLSYSNLPDYLKTCLLYLHMYPVGSIIWKDDLVKQLVAEGFIATREGKDQDQEMIE- KAAGLCFDALIDR RFIQPIYTKYNNKVLSCTVHEVVHDLIAQKSAEENFIVVADHNRKNIALSHKVRRLSLIFGDTIYAKTPANITK- SQIRSFRFFGLFE CMPCITEFKVLRVLNLQLSGHRGDNDPIDLTGISELFQLRYLKITSDVCIKLPNQMQKLQYLETLDIMDAPRVT- AVPWDIINLPHLL HLTLPVDTYLLDWISSMTDSVISLWTLGKLNYLQHLHLTSSSTRPSYHLERSVEALGYLIGGHGKLKTIVVAHV- SSAQNTVVRGAPE VTISWDRMSPPPLLQRFECPHSCFIFYRIPKWVTELGNLCILKIAVKELHMICLGTLRGLHALTDLSLYVETAP- IDKIIFDKAGFSV LKYCKLRFAAGIAWLKFEADAMPSLWKLMLVFNAIPRMDQNLVFFHHSRPAMHQRGGAVIIVEHMPGLRVISAK- FGGAASDLEYASR TVVSNHPSNPTINMQLVCYSSNGKRSRKRKQQPYDVVKGQPDEYAKRLERPAEKRISTPTKSSLRLHVPEITPK- PMQITDNNVCIRR EHMFDTVLTRGDVGMLNRLVVPKKHAEKYFPLDSSSTRTSKAIVLSFEDPAGKSWFFHYSYRSSSQNYVMFKGW- TGFVKEKFLEAGD TVSFSRGVGEATRGRLFIDCQNEQRYMFERVLTASDMESDGCSLMVPVNLVWPHPGLRKTIKGRHAVLQFEDGS- GNGKVWPFQFEAS GQYYLMKGLNYFVNDRDLAAGYTVSFYRAGTRLFVDSGRKDDKVALGTRSRERIYPKIVRSQ Brassica rapa LOC103849927 Cover 99% ident 80% CDS SEQ ID NO: 100 ATGTTGTTTGATAGTTCAGTGAGTGCTTCGTTGGGCACCATGAGACCACTTGTCAAGAAGCTCGACATGCTGCT- AGCTCCTGCTCGG GGATACAGTACCTTGTGCAAGAGGATCAAGGAAGTGATGCACCTTCTCAAACATGATGTTGAAGAGATAAGCTC- CTACCTTGATGAA CTTACAGAGGTGGAGGACCCTCCACCAATGGCCAAGTGCTGGATGAACGAGGCACGCGACCTGTCTTATGATAT- GGAGGATTACATT GATAGCTTGTTATTTGTGCCACCTGGCCATTTCATCAAGAAGAAGAAGAAGAAGAAGAAGAAGGGAAAGAAGAA- GATGGTGATAAAG AAGAGGCTCAAGTGGTGCAAACAGATCGTATTCACAAAGCAAGTGTCAGACCATGGTATCAAGACCAGTAAAAT- CATTCATGTTAAT GTCCCTCGTCTTCCCAATAAGCCCAAGGTTGCAAAAATAATATTACAGTTCAGGATCTATGTCCAGGAGGCTAT- TGAACGGTATGAC AAGTATAGGCTTCACCATTGCAGCACCTTGAGGCGTAGATTGTTGTCCACTGGTAGTATGCTTTCAGTGCCAAT- ACCCTATGAAGAA GCTGCCCAAATTGTAACTGATGGCCGGATGAATGAGTTTATCAGCTCACTGGCTGCTAATAATGCAGCAGATCA- GCAGCAGCTCAAG GTGGTATCTGTTCTTGGATCTGGGTGTCTAGGTAAAACTACGCTTGCGAATGTGTTGTACGACAGAATTGGGAT- GCAATTCGAATGC AGAGCTTTCATTCGAGTGTCCAAAAAGCCTGATATGAAGAGACTTTTCCGTGACTTGCTCTCGCAATTCCACCA- GAAGCAGCCACTG CCTACCAGTTGTAATGAGCTTGGCATAAGTGACAATATCATCAAACATCTGCAAGATAAAAGGTATCTAATTGT- TATTGATGATTTG TGGGATTTATCAGTATGGGATATTATTAAATATGCTTTTCCAAAGGGAAACCATGGAAGCAGAATAATAATAAC- TACACAGATTGAA GATGTTGCATTAACTTGTTGCTGTGATCACTCGGAGCATGTTTTCGAGATGAAACCTCTCAACATTGGTCACTC- AAGAGAGCTATTT TTTAATAGACTTTTTGGTTCTGAAAGTGACTGTCTTGAAGAATTCAAACGAGTTTCAAACGAAATTGTTGATAT- ATGTGGTGGTTTA CCGCTAGCAACAATCAACATAGCTAGTCATTTGGCAAACCAGGAGACAGAAGTATCATTGGATTTGCTAACAGA- CACACGTGATTTG TTGAGGTCCTGTTTGTGGTCAAATTCTACTTCAGAAAGAACAAAACAAGTACTGAACCTCAGCTACAGTAATCT- TCCTGATTATCTG AAGACATGTTTGCTGTATCTTCATATGTATCCAGTGGGCTCCATAATCTGGAAGGATGATCTGGTGAAGCAATT- GGTGGCTGAAGGG TTTATTGCTACAAGAGAAGGGAAAGACCAAGACCAAGAAATGATAGAGAAAGCTGCAGGACTCTGTTTCGATGC- ACTTATTGATAGA AGATTCATCCAGCCTATATATACCAAGTACAACAATAAGGTGTTGTCCTGCACGGTTCATGAGGTGGTACATGA- TCTTATTGCCCAA AAGTCTGCTGAAGAGAATTTCATTGTGGTAGCAGACCACAATCGAAAGAATATAGCACTTTCTCATAAGGTTCG- TCGACTATCTCTC ATCTTTGGCGACACAATATATGCCAAGACACCAGCAAACATCACAAAGTCACAAATTCGGTCATTCAGATTTTT- TGGATTATTCGAG TGTATGCCTTGTATTACAGAGTTCAAGGTTCTCCGTGTTCTAAACCTTCAACTATCTGGTCATCGTGGGGACAA- TGACCCTATAGAC CTCACTGGGATTTCAGAACTGTTTCAGCTGAGATATTTAAAGATTACAAGTGATGTGTGCATAAAACTACCAAA- TCAAATGCAAAAA CTGCAATATTTGGAAACGTTGGACATTATGGATGCACCAAGAGTCACTGCTGTTCCATGGGATATTATAAATCT- CCCACACCTGTTG CACCTGACTCTTCCTGTTGATACATATCTGCTGGATTGGATTAGCAGCATGACTGACTCCGTCATCAGTCTGTG- GACCCTTGGCAAG CTGAACTACCTGCAGCATCTTCATCTTACTAGTTCTTCTACACGTCCTTCATACCATCTGGAGAGAAGTGTGGA- GGCTCTGGGTTAT TTGATCGGAGGACATGGCAAGCTGAAAACTATAGTAGTCGCTCATGTCTCCTCTGCTCAAAATACTGTGGTTCG- TGGCGCCCCAGAA GTAACCATTTCATGGGATCGTATGTCACCTCCCCCCCTTCTCCAGAGATTCGAATGCCCACACAGCTGCTTCAT- ATTTTACCGAATT CCTAAGTGGGTTACAGAACTTGGCAACCTGTGCATTTTGAAGATTGCAGTGAAGGAGCTTCATATGATTTGTCT- TGGTACTCTCAGA GGATTGCATGCCCTCACTGATCTGTCGCTGTATGTGGAGACAGCGCCCATTGACAAGATCATCTTTGACAAGGC- CGGGTTCTCAGTT CTCAAGTACTGCAAATTGCGCTTCGCGGCTGGTATAGCTTGGCTGAAATTTGAGGCTGATGCAATGCCTAGTCT- ATGGAAACTGATG CTAGTTTTCAACGCCATCCCACGAATGGACCAAAATCTTGTTTTCTTTCACCACAGCCGACCGGCGATGCATCA- ACGTGGTGGTGCA GTAATCATTGTCGAGCATATGCCAGGGCTTAGAGTGATCTCCGCAAAATTTGGGGGCGCAGCTTCTGATCTAGA- GTATGCTTCGAGG ACCGTCGTTAGTAACCATCCAAGCAATCCTACAATCAACATGCAATTGGTGTGTTATAGTTCCAATGGTAAGAG- AAGCAGAAAAAGG AAACAACAACCTTACGACGTTGTGAAGGGACAACCAGATGAATACGCCAAGAGATTGGAGAGACCAGCTGAGAA- AAGGATTTCAACG CCGACAAAGTCTTCTTTGCGTCTGCATGTTCCAGAAATTACACCAAAACCTATGCAGATTACAGACAACAATGT- TCAGAGGAGGGAG CACATGTTCGATACGGTTCTGACTCGGGGGGACGTGGGGATGCTGAACCGGCTGGTGGTACCGAAGAAGCACGC- GGAGAAGTACTTC CCGCTGGACAGTTCCTCCACCCGCACCAGCAAGGCCATCGTACTCAGCTTTGAGGACCCTGCTGGGAAGTCATG- GTTCTTCCACTAC TCCTACCGGAGCAGCAGCCAGAACTACGTCATGTTCAAGGGGTGGACTGGCTTCGTCAAGGAGAAGTTTCTCGA- AGCCGGCGACACC GTCTCCTTCAGCCGCGGCGTCGGGGAGGCCACGAGGGGGAGGCTCTTCATCGACTGTCAAAATGAGCAGAGGTA- CATGTTCGAGCGA GTGCTGACGGCGAGTGATATGGAGTCGGATGGCTGCTCGCTGATGGTCCCAGTGAACTTGGTGTGGCCGCACCC- CGGCCTCCGCAAG ACGATCAAGGGGAGGCACGCCGTGCTGCAGTTTGAGGACGGCAGCGGCAACGGGAAGGTGTGGCCATTTCAGTT- TGAGGCCTCCGGC CAATACTATCTCATGAAGGGCTTGAACTACTTTGTTAACGACCGCGACCTTGCGGCTGGCTATACCGTCTCCTT-

CTACCGCGCCGGC ACGCGGTTGTTCGTCGACTCCGGGCGTAAAGATGACAAAGTAGCCTTGGGAACCAGAAGCCGCGAAAGGATCTA- TCCTAAGATCGTG CGGTCGCAGTAG LOC103849927 SEQ ID NO: 101 msgnhysrdihhntpsvhhhqnyavvdreylfeksltpsdvgklnrlvipkqhaekhfplnnagddvaaaette- kgmlltfedesgk cwkfrysywnssqsyvltkgwsryvkdkhlhagdvvffqrhrfdlhrvfigwrkrgevssptavsvvsqearvn- ttaywsglttpyr qvhastssypnihqeyshygavaeiptvvtgssrtvrlfgvnlechgdvvetppcpdgyngqhfyyystpdpmn- isfageameqvgd grr Bra034828 Cover 100% identity 79% SEQ ID NO: 102 MSVNHYSNTLSSHNHHNEHKESLFEKSLTPSDVGKLNRLVIPKQHAERYLPLNNCGGGGDVTAESTEKGVLLSF- EDESGKSWKFRYS YWNSSQSYVLTKGWSRYVKDKHLNAGDVVLFQRHRFDIHRLFIGWRRRGEASSSSAVSAVTQDPRANTTAYWNG- LTTPYRQVHASTS SYPNNIHQEYSHYGPVAETPTVAAGSSKTVRLFGVNLECHSDVVEPPPCPDAYNGQHIYYYSTPHPMNISFAGE- AMEQVGDGRG CDS SEQ ID NO: 103 ATGTCAGTCAACCATTACTCAAACACTCTCTCGTCGCACAATCACCACAACGAACATAAAGAGTCTTTGTTCGA- GAAGTCACTCACG CCAAGCGATGTTGGAAAGCTAAACCGTTTAGTCATACCAAAACAACACGCCGAGAGATACCTCCCTCTCAATAA- TTGCGGCGGCGGC GGCGACGTGACGGCGGAGTCGACGGAGAAAGGGGTGCTTCTCAGCTTCGAGGACGAGTCGGGAAAATCTTGGAA- ATTCAGATACTCA TATTGGAACAGTAGTCAAAGCTACGTGTTGACCAAAGGATGGAGCAGGTACGTCAAAGACAAGCACCTCAACGC- AGGGGACGTCGTT TTATTTCAACGGCACCGTTTTGATATTCATAGACTCTTCATTGGCTGGAGGAGACGCGGAGAGGCTTCTTCCTC- TTCCGCCGTTTCC GCCGTGACTCAAGATCCTCGAGCTAACACGACGGCGTACTGGAACGGTTTGACTACACCTTATCGTCAAGTACA- CGCGTCAACTAGT TCTTACCCTAACAACATCCACCAAGAGTATTCACATTATGGCCCTGTTGCTGAGACACCGACGGTAGCTGCAGG- GAGCTCGAAGACG GTGAGGCTATTTGGAGTTAACCTCGAATGTCACAGTGACGTTGTGGAGCCACCACCGTGTCCTGACGCCTACAA- CGGCCAACACATT TACTATTACTCAACTCCACATCCCATGAATATCTCATTTGCTGGAGAAGCAATGGAGCAGGTAGGAGATGGACG- AGGTTGA Bra005886 Cover 100% identity 79% SEQ ID NO: 104 MSVNHYSTDHHQVHHHHTLFLQNLHTTDTSEPTTTAATSLREDQKEYLFEKSLTPSDVGKLNRLVIPKQHAEKY- FPLNTIISNNAEE KGMLLSFEDESGKCWRFRYSYWNSSQSYVLTKGWSRYVKDKQLDPADVVFFQRQRSDSRRLFIGWRRRGQGSSS- AANTTSYSSSMTA PPYSNYSNRPAHSEYSHYGAAVATATETHFIPSSSAVGSSRTVRLFGVNLECQMDEDEGDDSVATAAAAECPRQ- DSYYDQNMYNYYT PHSSAS CDS 105 ATGTCAGTCAACCATTACTCCACGGACCACCACCAGGTCCACCACCACCACACTCTCTTCTTGCAGAACCTCCA- CACCACCGACACA TCGGAGCCAACCACAACCGCCGCCACATCACTCCGCGAAGACCAGAAAGAGTATCTCTTCGAGAAATCTCTCAC- ACCAAGCGACGTT GGCAAACTCAACCGTCTCGTTATACCAAAACAGCACGCGGAGAAGTACTTCCCTCTCAACACCATCATCTCCAA- TAATGCTGAGGAG AAAGGGATGCTTCTAAGCTTCGAAGACGAGTCAGGCAAGTGCTGGAGGTTCAGATACTCTTACTGGAACAGCAG- TCAAAGCTACGTG TTGACTAAAGGATGGAGCAGATACGTCAAAGACAAACAGCTCGACCCAGCCGATGTTGTTTTCTTCCAACGTCA- ACGTTCTGATTCC CGGAGACTCTTTATTGGCTGGCGTAGACGCGGTCAAGGCTCCTCCTCCGCCGCGAATACGACGTCGTATTCTAG- TTCCATGACTGCT CCACCGTATAGTAATTACTCTAATCGTCCTGCTCACTCAGAGTATTCCCACTATGGCGCCGCCGTAGCAACAGC- GACGGAGACGCAC TTCATACCATCGTCTTCCGCCGTCGGGAGCTCGAGGACGGTGAGGCTTTTTGGTGTGAATTTGGAGTGTCAAAT- GGATGAAGACGAA GGAGATGATTCGGTTGCCACGGCAGCCGCCGCTGAGTGTCCTCGTCAGGACAGCTACTACGACCAAAACATGTA- CAATTATTACACT CCTCACTCCTCAGCCTCATAA Bra005301 Cover 100% identity 58% SEQ ID NO: 106 MSINQYSSDFNYHSLMWQQQQHRHHHHQNDVAEEKEALFEKPLTPSDVGKLNRLVIPKQHAERYFPLAAAAADA- MEKGLLLCFEDEE GKPWRFRYSYWNSSQSYVLTKGWSRYVKEKQLDAGDVILFHRHRVDGGRFFIGWRRRGNSSSSSDSYRHLQSNA- SLQYYPHAGVQAV ESQRGNSKTLRLFGVNMECQLDSDLPDPSTPDGSTICPTSHDQFHLYPQQHYPPPYYMDISFTGDVHQTRSPQG CDS SEQ ID NO: 107 ATGTCAATAAACCAATACTCAAGCGATTTCAACTACCACTCTCTCATGTGGCAACAACAGCAGCACCGCCACCA- CCACCATCAAAAC GACGTCGCGGAGGAAAAAGAAGCTCTTTTCGAGAAACCCTTAACCCCAAGTGACGTCGGAAAACTCAACCGCCT- CGTCATCCCAAAA CAGCACGCCGAGAGATACTTCCCTCTCGCAGCAGCCGCCGCAGACGCGATGGAGAAGGGATTACTTCTCTGCTT- CGAGGACGAGGAA GGTAAGCCATGGAGATTCAGATACTCGTATTGGAACAGTAGCCAGAGTTATGTCTTGACCAAAGGATGGAGCAG- ATACGTCAAGGAG AAGCAGCTCGACGCCGGTGACGTCATTCTCTTCCACCGCCACCGTGTTGACGGAGGAAGATTCTTCATTGGCTG- GAGAAGACGCGGC AACTCTTCCTCCTCTTCCGACTCTTATCGCCATCTTCAGTCCAATGCCTCGCTCCAATATTATCCTCATGCAGG- AGTTCAAGCGGTG GAGAGCCAGAGAGGGAATTCGAAGACATTAAGACTGTTCGGAGTGAACATGGAGTGTCAGCTAGACTCCGACTT- GCCCGATCCATCT ACACCAGACGGTTCCACCATATGTCCGACCAGTCACGACCAGTTTCATCTCTACCCTCAACAACACTATCCTCC- TCCGTACTACATG GACATAAGTTTCACAGGAGATGTGCACCAGACGAGAAGCCCACAAGGATAA Bra017262 Cover 92% identity 56% SEQ ID NO: 108 MSINQYSSEFYYHSLMWQQQQQHHHQNEVVEEKEALFEKPLTPSDVGKLNRLVIPKQHAERYFPLAAAAVDAVE- KGLLLCFEDEEGK PWRFRYSYWNSSQSYVLTKGWSRYVKEKQLDAGDVVLFHRHRADGGRFFIGWRRRGDSSSSSDSYRNLQSNSSL- QYYPHAGAQAVEN QRGNSKTLRLFGVNMECQIDSDWSEPSTPDGFTTCPTNHDQFPIYPEHFPPPYYMDVSFTGDVHQTSSQQG CDS SEQ ID NO: 109 ATGTCAATAAATCAATATTCAAGCGAGTTCTACTACCATTCTCTCATGTGGCAACAACAGCAGCAACACCACCA- TCAAAACGAAGTC GTGGAGGAAAAAGAAGCTCTTTTCGAGAAACCCTTAACCCCAAGTGACGTCGGAAAACTAAACCGCCTAGTCAT- CCCTAAACAGCAC GCCGAGAGATACTTCCCTCTCGCCGCCGCCGCGGTAGACGCCGTGGAGAAGGGATTACTCCTCTGCTTCGAGGA- CGAGGAAGGTAAG CCATGGAGATTCAGATACTCTTATTGGAATAGTAGCCAGAGTTACGTCTTGACCAAAGGATGGAGCAGATATGT- TAAAGAGAAGCAA CTTGACGCCGGCGACGTTGTTCTCTTTCATCGCCACCGTGCTGACGGTGGAAGATTCTTCATTGGCTGGAGAAG- ACGCGGCGACTCT TCCTCCTCCTCCGACTCTTATCGCAATCTTCAATCTAATTCCTCGCTCCAATATTATCCTCATGCAGGGGCTCA- AGCGGTGGAGAAC CAGAGAGGTAACTCCAAGACATTGAGACTTTTTGGAGTGAACATGGAGTGCCAGATAGACTCAGACTGGTCCGA- GCCATCCACACCT GACGGTTTTACCACATGTCCAACCAATCACGACCAGTTTCCTATCTACCCTGAACACTTTCCTCCTCCGTACTA- CATGGACGTAAGT TTCACAGGAGATGTGCACCAGACGAGTAGCCAACAAGGATAG Bra000434 Cover 96% identity 47% SEQ ID NO: 110 MMTNLSLAREGEEEEEEAGAKKPTEEVEREHMFDKVVTPSDVGKLNRLVIPKQHAERYFPLDSSTNEKGLILNF- EDLTGKSWRFRYS YWNSSQSYVMTKGWSRFVKDKKLDAGDIVSFLRCVGDTGRDSRLFIDWRRRPKVPDYTTSTSHFPAGAMFPRFY- SFQTATTSTSYNP YNHQQPRHHHSGYCYPQIPREFGYGYVVRSVDQRAVVADPLVIESVPVMMHGGARVNQAAVGTAGKRLRLFGVD- MECGESGGTNSTE EESSSSGGSLPRGGASPSSSMFQLRLGNSSEDDHLFKKGKSSLPFNLDQ SEQ ID NO: 111 ATGATGACAAATTTGTCTCTTGCAAGAGAAGGAGAAGAAGAAGAAGAAGAGGCAGGAGCAAAGAAGCCCACAGA- AGAAGTGGAGAGA GAGCACATGTTCGACAAAGTGGTGACTCCAAGTGACGTCGGGAAACTAAACCGACTCGTGATCCCAAAGCAACA- CGCGGAGAGATAC TTCCCTTTAGATTCATCCACAAACGAGAAGGGTTTGATTCTAAACTTCGAAGATCTCACGGGAAAGTCATGGAG- GTTCCGTTACTCT TACTGGAACAGCAGTCAGAGCTATGTCATGACTAAAGGTTGGAGCCGTTTCGTTAAAGACAAGAAGCTAGACGC- TGGAGATATTGTC TCTTTCCTGAGATGTGTCGGAGACACAGGAAGGGACAGCCGCTTGTTTATCGATTGGAGGAGACGACCTAAAGT- CCCTGACTACACG ACATCGACTTCTCACTTTCCTGCCGGAGCTATGTTCCCTAGGTTTTACAGTTTTCAGACAGCAACTACTTCCAC- AAGTTACAATCCC TATAATCATCAGCAGCCACGTCATCATCACAGTGGTTACTGTTATCCTCAAATCCCGAGAGAATTTGGATATGG- GTATGTCGTTAGG TCAGTAGATCAGAGGGCGGTGGTGGCTGATCCGTTAGTGATCGAATCTGTGCCGGTGATGATGCACGGAGGAGC- TCGAGTGAACCAG GCGGCTGTTGGAACGGCCGGGAAAAGGCTGAGGCTTTTTGGAGTCGATATGGAATGTGGCGAGAGTGGAGGAAC- AAACAGTACGGAG GAAGAATCTTCATCTTCCGGTGGGAGTTTGCCACGTGGCGGTGCTTCTCCGTCTTCCTCTATGTTTCAGCTGAG- GCTTGGAAACAGC AGTGAAGATGATCACTTATTTAAGAAAGGAAAGTCTTCATTGCCTTTTAATTTGGATCAATAA Bra040478 Cover 96% identity 48% SEQ ID NO: 112 MMTNLSLAREGEAQVKKPIEEVEREHMFDKVVTPSDVGKLNRLVIPKQHAERYFPLDSSSNEKGLLLNFEDLTG- KSWRFRYSYWNSS QSYVMTKGWSRFVKDKKLDAGDIVSFQRCVGDSRLFIDWRRRPKVPDYPTSTAHFAAGAMFPRFYSFPTATTST- CYDLYNHQPPRHH HIGYGYPCLIPREFGYGYFVRSVDQRAVVADPLVIESVPVMMRGGARVSQEVVGTAGKRLRLFGVDMEEESSSS- GGSLPRAGGGGAS SSSSLFQLRLGSSCEDDHFSKKGKSSLPFDLDQ SEQ ID NO: 113 ATGATGACCAACTTGTCTCTTGCAAGGGAAGGAGAAGCACAAGTAAAGAAGCCCATAGAAGAAGTTGAGAGAGA- GCACATGTTCGAC AAAGTGGTGACTCCAAGCGACGTAGGGAAACTAAACAGACTCGTGATCCCAAAGCAACACGCAGAGAGATACTT- CCCTCTAGATTCA TCCTCAAACGAGAAAGGTTTGCTTCTAAACTTTGAAGATCTAACAGGAAAGTCATGGAGGTTCCGTTACTCTTA- CTGGAACAGTAGC CAGAGCTATGTCATGACTAAAGGTTGGAGTCGTTTCGTTAAAGACAAGAAGCTTGACGCCGGAGATATTGTCTC- TTTCCAGAGATGT GTCGGAGACAGCCGCTTGTTTATCGATTGGAGGAGACGACCTAAAGTCCCTGACTATCCGACATCGACTGCTCA- CTTTGCTGCAGGA GCTATGTTCCCTAGGTTTTACAGTTTTCCGACAGCAACTACTTCGACATGTTACGATCTGTACAATCATCAGCC- GCCACGTCATCAT CACATTGGTTACGGTTATCCACAGATTCCGAGAGAATTTGGATACGGGTATTTCGTTAGGTCAGTGGACCAGAG- AGCGGTGGTGGCT GATCCGTTGGTGATCGAATCTGTGCCGGTGATGATGCGCGGAGGAGCTCGAGTTAGTCAGGAGGTTGTTGGAAC- GGCCGGGAAGAGG CTGAGGCTTTTTGGAGTCGATATGGAGGAAGAATCTTCATCTTCCGGTGGGAGTTTGCCGCGTGCCGGAGGTGG- CGGTGCTTCTTCA TCTTCCTCTTTGTTTCAGCTGAGACTTGGGAGCAGCTGTGAAGATGATCACTTCTCTAAGAAAGGAAAGTCTTC- ATTGCCTTTTGAT TTGGATCAATAA Bra004501 Cover 74% identity 45% SEQ ID NO: 114 MMMTNLSLSREGEEEEEEEQEEAKKPMEEVEREHMFDKVVTPSDVGKLNRLVIPKQYAERYFPLDSSTNEKGLL- LNFEDLAGKSWRF RYSYWNSSQSYVMTKGWSRFVKDKKLDAGDIVSFQRCVGDSGRDSRLFIDWRRRPKVPDHPTSIAHFAAGSMFP- RFYSFPTATSYNL YNYQQPRHHHHSGYNYPQIPREFGYGYLVDQRAVVADPLVIESVPVMMHGGAQVSQAVVGTAGKRLRLFGVDME- EESSSSGGSLPRG DASPSSSLFQLRLGSSSEDDHFSKKGKSSLPFDLDQ SEQ ID NO: 133 ATGATGATGACAAACTTGTCTCTTTCAAGAGAAGGAGAAGAGGAGGAAGAAGAAGAACAAGAAGAGGCCAAGAA- GCCCATGGAAGAA GTAGAGAGAGAGCACATGTTCGACAAAGTGGTGACTCCAAGCGATGTTGGTAAACTAAACCGGCTCGTGATCCC- AAAGCAATACGCA GAGAGATACTTCCCTTTAGATTCATCCACAAACGAGAAAGGTTTGCTTCTAAACTTCGAAGATCTCGCAGGAAA- GTCATGGAGGTTC CGTTACTCTTACTGGAACAGTAGTCAGAGCTATGTCATGACTAAAGGTTGGAGCCGTTTCGTTAAAGACAAAAA- GCTAGACGCCGGA GATATTGTCTCTTTCCAGAGATGTGTCGGAGATTCAGGAAGAGACAGCCGCTTGTTTATTGATTGGAGGAGAAG- ACCTAAAGTTCCT GACCATCCGACATCGATTGCTCACTTTGCTGCCGGATCTATGTTTCCTAGGTTTTACAGTTTTCCGACAGCAAC- TAGTTACAATCTT TACAACTATCAGCAGCCACGTCATCATCATCACAGTGGTTATAATTATCCTCAAATTCCGAGAGAATTTGGATA- CGGGTACTTGGTG GATCAAAGAGCCGTGGTGGCTGATCCGTTGGTGATTGAATCTGTGCCGGTGATGATGCACGGAGGAGCTCAAGT- TAGTCAGGCGGTT GTTGGAACGGCCGGGAAGAGGCTGAGGCTTTTTGGAGTCGATATGGAGGAAGAATCTTCATCTTCCGGTGGGAG- TTTGCCACGTGGT GACGCTTCTCCGTCTTCCTCTTTGTTTCAGCTGAGACTTGGAAGCAGCAGTGAAGATGATCACTTCTCTAAGAA- AGGAAAGTCCTCA TTGCCTTTTGATTTGGATCAATAA Bra003482 Cover 79% identity 44% SEQ ID NO: 115 MNQEEENPVEKASSMEREHMFEKVVTPSDVGKLNRLVIPKQHAERYFPLDNNSDSSKGLLLNFEDRTGNSWRFR- YSYWNSSQSYVMT KGWSRFVKDKKLDAGDIVSFQRDPGNKDKLFIDWRRRPKIPDHHHQFAGAMFPRFYSFSHPQNLYHRYQQDLGI-

GYYVSSMERNDPT AVIESVPLIMQRRAAHVAAIPSSRGEKRLRLFGVDMECGGGGGSVNSTEEESSSSGGGGGVSMASVGSLLQLRL- VSSDDESLVAMEA ASVDEDHHLFTKKGKSSLSFDLDRK SEQ ID NO: 116 ATGAATCAAGAAGAAGAGAATCCTGTGGAAAAAGCCTCTTCAATGGAGAGAGAGCACATGTTTGAAAAAGTAGT- AACACCAAGCGAC GTAGGCAAACTAAACCGACTCGTGATCCCAAAGCAACACGCGGAGAGATACTTCCCTTTAGACAACAATTCTGA- CAGCAGCAAAGGT TTGCTTCTAAACTTCGAAGACCGAACAGGAAACTCATGGAGATTCCGTTACTCTTACTGGAACAGTAGCCAGAG- TTATGTCATGACA AAAGGTTGGAGCCGCTTCGTCAAAGACAAGAAGCTTGATGCTGGCGACATCGTTTCTTTTCAGAGAGATCCTGG- TAATAAAGACAAG CTTTTCATTGATTGGAGGAGACGACCAAAGATTCCAGATCATCATCATCAATTCGCTGGAGCTATGTTCCCTAG- GTTTTACTCTTTC TCTCATCCTCAGAACCTTTATCATCGATATCAACAAGATCTTGGAATTGGGTATTATGTGAGTTCAATGGAGAG- AAATGATCCAACG GCTGTAATTGAATCTGTGCCGTTGATAATGCAAAGGAGAGCAGCACACGTGGCTGCTATACCTTCATCAAGAGG- AGAGAAGAGGTTA AGGCTGTTTGGAGTGGACATGGAGTGCGGCGGCGGCGGAGGAAGTGTGAATAGCACGGAGGAAGAGTCGTCGTC- TTCCGGTGGTGGC GGCGGCGTTTCTATGGCTAGTGTTGGTTCTCTTCTCCAATTGAGGCTAGTGAGCAGTGATGATGAGTCTTTGGT- AGCAATGGAAGCT GCAAGTGTCGATGAGGATCATCACTTGTTTACAAAGAAAGGAAAGTCTTCTTTGTCTTTCGATTTGGATAGAAA- ATGA Bra007646 Cover 74% identity 45% SEQ ID NO: 117 MNQENKKPLEEASTSMERENMFDKVVTPSDVGKLNRLVIPKQHAERYFPLDNSSTNNKGLLLDFEDRTGSSWRF- RYSYWNSSQSYVM TKGWSRFVKDKKLDAGDIVSFQRDPCNKDKLYIDWRRRPKIPDHHQFAGAMFPRFYSFPHPQMPTSFESSHNLY- HHRFQRDLGIGYY PTAVIESVPVIMQRREAQVANMASSRGEKRLRLFGVDVECGGGGGGSVNSTEEESSSSGGSMSRGGVSMAGVGS- LLQLRLVSSDDES LVAMEGATVDEDHHLFTTKKGKSSLSFDLDI CDS SEQ ID NO: 118 ATGAATCAAGAAAACAAGAAGCCTTTGGAAGAAGCTTCGACTTCAATGGAGAGAGAGAACATGTTCGACAAAGT- AGTAACACCAAGC GACGTAGGGAAACTAAACCGACTCGTGATCCCAAAGCAACACGCAGAGAGATACTTCCCTTTAGACAACTCCTC- AACAAACAACAAA GGGTTGCTTCTAGACTTCGAAGACCGTACAGGAAGCTCATGGAGATTCCGTTACTCTTACTGGAACAGTAGCCA- AAGTTATGTCATG ACAAAAGGTTGGAGCCGTTTTGTCAAAGACAAGAAGCTTGATGCTGGTGACATCGTGTCTTTTCAAAGAGATCC- CTGTAATAAAGAC AAGCTTTACATAGATTGGAGGAGACGACCAAAGATTCCAGATCATCATCAGTTCGCCGGAGCTATGTTCCCTAG- GTTTTACTCTTTC CCTCACCCTCAGATGCCGACAAGTTTTGAAAGTAGTCACAACCTTTATCATCATCGGTTTCAACGAGATCTTGG- AATTGGGTATTAT CCAACGGCTGTGATTGAATCTGTGCCGGTGATAATGCAAAGGAGAGAAGCACAAGTGGCTAATATGGCTTCATC- AAGAGGAGAGAAG AGGTTAAGGCTGTTTGGAGTGGACGTGGAGTGCGGCGGCGGAGGAGGAGGAAGTGTGAATAGCACGGAGGAAGA- GTCGTCGTCTTCC GGTGGTAGTATGTCACGTGGCGGCGTTTCTATGGCTGGTGTTGGTTCTCTCCTTCAGTTGAGGTTAGTGAGCAG- TGATGATGAGTCT TTAGTAGCGATGGAAGGTGCTACTGTCGATGAGGATCATCACTTGTTTACAACTAAGAAAGGAAAGTCTTCTTT- GTCTTTCGATTTG GATATATGA Bra014415 Cover 48% identity 60% SEQ ID NO: 119 MERKSNDLERSENIDSQNKKMNLEEERPVQEASSMEREHMFDKVVTPSDVGKLNRLVIPKQHAERYFPLDNNSS- DNNKGLLLNFEDR IGILWSFRYSYWNSSQSYVMTKGWSRFVKDKKLDAGDIVSFHRGSCNKDKLFIDWKRRPKIPDHQVVGAMFPRF- YSYPYPQIQASYE RHNLYHRYQRDIGIGYYVRSMERYDPTAVIESVPVIMQRRAHVATMASSRGEKRLRLFGVDMECVRGGRGGGGS- VNSTEEESSTSGG SISRGGVSMAGVGSPLQLRLVSSDGDDQSLVARGAARVDEDHHLFTKKGKSSLSFDLDK CDS SEQ ID NO: 120 ATGGAGAGGAAGTCCAATGATCTTGAGAGATCTGAGAATATTGATTCTCAAAACAAGAAGATGAATCTAGAAGA- AGAGAGGCCTGTA CAAGAAGCTTCTTCGATGGAGAGAGAGCACATGTTCGACAAAGTAGTAACACCAAGCGACGTTGGGAAACTAAA- CCGGCTGGTGATC CCAAAGCAACACGCAGAGCGATACTTCCCTTTAGACAATAATTCCTCAGACAACAACAAAGGTTTGCTTCTAAA- CTTCGAAGATCGA ATAGGAATCTTATGGAGTTTCCGTTACTCCTACTGGAACAGTAGCCAAAGTTATGTAATGACTAAAGGCTGGAG- CCGTTTCGTCAAA GACAAGAAGCTTGATGCTGGCGACATAGTTTCTTTTCATAGAGGTTCTTGTAATAAAGACAAGCTTTTCATTGA- TTGGAAGAGACGA CCAAAGATTCCTGATCACCAAGTCGTCGGAGCTATGTTCCCTAGGTTTTACTCTTACCCTTATCCTCAGATACA- GGCTAGTTATGAA CGTCACAACCTTTATCATCGATATCAACGAGATATAGGAATTGGGTATTATGTGAGGTCAATGGAGAGATATGA- TCCAACGGCTGTA ATTGAATCTGTGCCGGTGATAATGCAAAGGAGAGCACATGTGGCTACTATGGCTTCATCAAGAGGAGAGAAGAG- GTTAAGGCTTTTT GGAGTGGATATGGAGTGCGTCAGAGGCGGCCGAGGAGGAGGAGGAAGTGTGAATAGCACGGAGGAAGAGTCTTC- GACTTCCGGTGGT AGTATCTCACGTGGCGGCGTTTCTATGGCTGGTGTTGGCTCTCCACTCCAGTTGAGGTTAGTGAGCAGTGACGG- TGATGATCAGTCT CTAGTAGCTAGGGGAGCTGCTAGGGTTGATGAGGATCATCACTTGTTTACAAAGAAAGGAAAGTCTTCTTTGTC- TTTCGATTTGGAT AAATGA Bra038346 Cover 51% identity 57% SEQ ID NO: 121 MVFSCIDESSSTSESFSPATATATATATKFSAPPLPPLRLNRMRSGGSNVVLDSKNGVDIDSRKLSSSKYKGVV- PQPNGRWGAQIYV KHQRVWLGTFCDEEEAAHSYDIAARKFRGRDAVVNFKTFLASEDDNGELCFLEAHSKAEIVDMLRKHTYADELA- QSNKRSGANTNTN TTQSHTVSRTREVLFEKVVTPSDVGKLNRLVIPKQHAEKYFPLPSLSVTKGVLINFEDVTGKVWRFRYSYWNSS- QSYVLTKGWSRFV KEKNLRAGDVVTFERSTGSDRQLYIDWKIRSGPSKNPVQVVVRLFGVDIFNVTSAKPSNVVDACGGKRSRDVDM- FALRCSKKHAIIN AL CDS SEQ ID NO: 122 ATGGTATTCAGTTGCATAGACGAGAGCTCTTCCACTTCAGAATCTTTTTCACCCGCAACCGCAACCGCAACCGC- AACCGCCACAAAG TTCTCTGCTCCTCCGCTTCCACCGTTACGCCTCAACCGGATGAGAAGCGGTGGAAGCAACGTCGTGTTGGATTC- AAAGAATGGCGTA GATATTGATTCACGGAAGCTATCGTCGTCAAAGTACAAAGGCGTGGTTCCTCAGCCCAACGGAAGATGGGGAGC- TCAGATTTACGTG AAGCACCAGCGAGTTTGGCTGGGCACTTTCTGCGATGAAGAGGAAGCTGCTCACTCCTACGACATAGCCGCCCG- TAAATTCCGTGGC CGTGACGCCGTTGTCAACTTCAAAACCTTCCTCGCCTCAGAGGACGACAACGGCGAGTTATGTTTCCTTGAAGC- TCACTCCAAGGCC GAGATCGTCGACATGTTGAGGAAACACACTTACGCTGACGAGCTTGCGCAGAGCAATAAACGCAGCGGAGCGAA- TACGAATACGAAT ACGACTCAAAGCCACACCGTTTCGAGAACACGTGAAGTGCTTTTCGAGAAGGTTGTCACGCCTAGCGACGTTGG- TAAGCTAAACCGC CTCGTGATACCTAAACAGCACGCGGAGAAATATTTTCCGTTACCGTCACTGTCGGTGACTAAAGGCGTTCTGAT- CAACTTCGAAGAC GTGACGGGTAAGGTGTGGCGGTTCCGTTACTCATACTGGAACAGTAGTCAAAGTTACGTGTTGACCAAGGGATG- GAGTCGGTTCGTT AAGGAGAAGAATCTCCGAGCCGGTGATGTCGTTACTTTCGAGAGATCGACCGGTTCAGACCGGCAGCTTTATAT- TGATTGGAAAATC CGGTCTGGTCCGAGCAAAAACCCTGTTCAGGTTGTGGTTAGGCTTTTCGGAGTTGACATCTTCAACGTGACAAG- CGCGAAGCCGAGC AACGTTGTAGACGCGTGCGGTGGAAAGAGATCTCGGGATGTTGATATGTTTGCGCTACGGTGTTCCAAAAAACA- CGCTATAATCAAT GCTTTGTGA Zea mays GRMZM2G053008 Cover 74% identity 47% SEQ ID NO: 123 MAASPSSPLTAPPEPVTPPSPWTITDGAISGTLPAAEAFAVHYPGYPSSPARAARTLGGLPGLAKVRSSDPGAR- LELRFRPEDPYCH PAFGQSRASTGLLLRLSKRKGAAAPCAHVVARVRTAYYFEGMADFQHVVPVHAAQTRKRKHSDSQNDNENFGSD- KTGHDEADGDVMM LVPPLFSVKDRPTKIALVPSSNAISKTMHRGVVQERWEMNVGPTLALPFNTQVVPEKINWEDHIRKNSVEWGWQ- MAVCKLFDERPVW PRQSLYERFLDDNVHVSQNQFKRLLFRAGYYFSTGPFGKFWIRRGYDPRKDSESQIYQRIDFRMPPELRYLLRL- KNSESRKWADMCK LETMPSQSFIYLQLYELKDDFIQAEIRKPSYQSVCSRSTGWFSKPMIKTLRLQVSIRLLSLLHNEEAKNLLRNA- HELIERSKKQEAL SRSELSIEYNDADQVSAAHTGTEDQVGPNNSDSEDVDDEEEEEELEGYDSPPMADDIHEFTLGDSYAFGEGFSN- GYLEEVLRSLPLQ EDGQKKLCDAPINADASD CDS SEQ ID NO: 124 ATGGCCGCCTCGCCCTCTTCACCCTTGACAGCGCCGCCAGAGCCGGTGACCCCGCCGTCCCCATGGACCATCAC- AGACGGAGCCATC TCTGGCACGCTCCCAGCAGCCGAGGCCTTCGCAGTGCACTACCCGGGCTACCCCTCCTCTCCCGCCCGCGCCGC- CCGCACCCTCGGC GGTCTCCCCGGCCTCGCCAAGGTCCGGAGTTCCGATCCCGGCGCCCGCCTCGAGCTCCGCTTCCGCCCCGAGGA- CCCCTACTGCCAT CCAGCCTTTGGCCAGTCCCGCGCCTCCACTGGCCTTCTGCTGCGCCTCTCCAAGCGCAAAGGAGCTGCGGCACC- TTGTGCCCATGTG GTCGCTCGTGTCCGGACTGCTTACTACTTCGAAGGTATGGCAGATTTTCAACATGTTGTTCCAGTGCATGCTGC- ACAAACAAGAAAA AGAAAACACTCAGATTCTCAAAATGATAATGAGAATTTTGGTAGTGATAAGACAGGACATGATGAAGCAGATGG- AGATGTCATGATG TTGGTACCCCCTCTCTTTTCAGTGAAGGATAGGCCAACAAAGATAGCGCTTGTACCATCGTCCAATGCCATATC- TAAAACCATGCAC AGGGGAGTTGTACAAGAACGGTGGGAGATGAATGTTGGACCAACTCTGGCGCTTCCGTTCAACACTCAAGTTGT- CCCGGAGAAGATT AATTGGGAAGACCACATTAGAAAGAATTCTGTAGAATGGGGTTGGCAAATGGCTGTTTGCAAATTGTTTGATGA- GCGCCCTGTGTGG CCAAGGCAATCACTTTATGAGCGGTTCCTTGATGATAATGTGCATGTCTCTCAAAACCAATTCAAAAGGCTTCT- GTTTAGAGCTGGA TACTACTTCTCTACTGGACCCTTTGGAAAATTTTGGATCAGAAGAGGATATGACCCTCGTAAAGACTCTGAGTC- ACAAATATATCAG AGAATTGATTTTCGCATGCCTCCCGAGCTACGATATCTTCTAAGGCTGAAGAATTCTGAGTCTCGAAAGTGGGC- AGATATGTGCAAG CTTGAAACAATGCCATCACAGAGTTTCATCTACCTGCAATTATATGAACTGAAGGATGATTTTATTCAAGCAGA- AATTCGAAAACCT TCTTATCAATCAGTTTGTTCACGTTCTACAGGATGGTTTTCTAAGCCAATGATCAAAACCCTGAGGTTGCAAGT- GAGCATAAGGCTC CTCTCTTTATTGCATAATGAAGAGGCTAAAAACTTGTTGAGGAATGCCCATGAGCTTATTGAAAGGTCCAAGAA- GCAGGAAGCCCTT TCGAGATCTGAGCTGTCAATAGAATATAATGATGCTGATCAAGTTTCTGCCGCACATACTGGAACTGAGGATCA- AGTCGGCCCTAAC AACTCTGATAGTGAAGATGTGGATGATGAAGAAGAGGAAGAGGAATTGGAGGGTTATGATTCTCCACCTATGGC- AGATGATATTCAT GAGTTCACCTTAGGTGATTCCTATGCATTTGGTGAAGGCTTCTCGAATGGATACCTCGAAGAAGTACTGCGCAG- CTTGCCATTGCAG GAAGACGGCCAAAAGAAATTATGTGATGCTCCTATCAACGCTGATGCAAGTGATGGAGAGTTTGAAATTTACGA- ACAGCCCAGTGAT GATGAAGATTCTGATGGCTAG GRMZM2G102059_T01 Cover 47% identity 62% SEQ ID NO: 125 MEFASSSSRFSREEDEEEEQEEEEEEEEASPREIPFMTAAATADTGAAASSSSPSAAASSGPAAAPRSSDGAGA- SGSGGGGSDDVQV IEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDAAANEKGQLLSFEDRAGKLWRFRYSYWNSSQSYVMTKG- WSRFVKEKRLDAG DTVSFCRGAGDTARDRLFIDWKRRADSRDPHRMPRLPLPMAPVASPYGPWGGGGGGGAGGFFMPPAPPATLYEH- HRFRQALDFRNIN AAAAPARQLLFFGSAGMPPRASMPQQQQPPPPPHPPLHSIMLVQPSPAPPTASVPMLLDSVPLVNSPTAASKRV- RLFGVNLDNPQPG TSAESSQDANALSLRTPGWQRPGPLRFFESPQRGAESSAASSPSSSSSSKREAHSSLDLDL CDS SEQ ID NO: 126 ATGGAGTTCGCGAGCTCTTCGAGTAGGTTTTCCAGGGAGGAGGACGAGGAGGAAGAGCAGGAGGAAGAGGAGGA- GGAGGAGGAGGCG TCTCCGCGCGAGATCCCCTTCATGACAGCGGCAGCGACGGCCGACACCGGAGCCGCCGCCTCCTCGTCCTCGCC- TTCCGCGGCGGCC TCATCGGGTCCTGCTGCTGCCCCCCGCTCGAGCGACGGCGCCGGGGCGTCCGGGAGCGGCGGCGGCGGGAGCGA- CGACGTGCAGGTG ATCGAGAAGGAGCACATGTTCGACAAGGTGGTGACGCCCAGCGACGTGGGGAAGCTCAACCGGCTGGTGATCCC- GAAGCAGCACGCG GAGAAGTACTTCCCGCTGGACGCGGCGGCCAACGAGAAGGGCCAGCTGCTCAGCTTCGAGGACCGCGCCGGTAA- GCTCTGGCGCTTC CGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTCATGACCAAGGGCTGGAGCCGCTTCGTCAAGGAGAAGCG- CCTCGACGCCGGC GACACCGTCTCCTTCTGCCGCGGCGCCGGCGACACCGCGCGGGACCGCCTCTTCATCGACTGGAAGCGCCGCGC- CGACTCCCGCGAC CCGCACCGCATGCCGCGCCTCCCGCTCCCCATGGCGCCCGTCGCGTCGCCCTACGGCCCCTGGGGCGGCGGCGG- CGGCGGCGGCGCG GGCGGTTTCTTCATGCCGCCCGCGCCGCCCGCCACACTCTACGAGCACCACCGCTTCCGCCAGGCCCTCGACTT- CCGCAACATCAAC GCCGCGGCCGCGCCGGCCAGGCAGCTCCTCTTCTTCGGCTCAGCCGGCATGCCCCCGCGCGCGTCCATGCCGCA- GCAGCAGCAGCCG CCTCCGCCCCCGCACCCGCCTCTGCACAGCATTATGTTGGTGCAACCCAGCCCCGCGCCGCCCACGGCCAGCGT- GCCCATGCTTCTC GACTCGGTACCGCTCGTCAACAGCCCAACGGCAGCGTCGAAGCGCGTCCGCCTGTTTGGGGTCAACCTCGACAA- CCCGCAACCAGGC ACAAGTGCGGAGTCAAGCCAAGATGCCAACGCATTGTCGCTGAGGACACCGGGATGGCAAAGGCCGGGGCCGTT- GAGGTTCTTCGAA TCGCCTCAACGCGGCGCCGAGTCATCTGCAGCCTCCTCGCCGTCGTCATCGTCGTCCTCCAAGAGAGAAGCGCA- CTCGTCCTTGGAT CTCGATCTGTGA GRMZM2G098443_T01 Cover 47% identity 63% SEQ ID NO: 127 MEFTTPPPATRSGGGEERAAAEHNQHHQQQHATVEKEHMFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDAAANE-

KGLLLSFEDRTGK PWRFRYSYWNSSQSYVMTKGWSRFVKEKRLDAGDTVSFGRGISEAARDRLFIDWRCRPDPPVVHHQYHHRLPLP- SAVVPYAPWAAHA HHHHYPADGHTEPVTPCLCATLVATEMRASSSQLSLTRSNLSRPPQPRIARVDGAQPRPSSSPRQPQSLWCRSC- QPQPRRTADVP CDS SEQ ID NO: 128 ATGGAGTTCACCACTCCCCCGCCCGCGACCCGGTCGGGCGGCGGAGAGGAGAGGGCGGCTGCTGAGCACAACCA- GCACCACCAGCAG CAGCATGCGACGGTGGAGAAGGAGCACATGTTCGACAAGGTGGTGACGCCGAGCGACGTCGGGAAGCTGAACCG- GCTGGTGATCCCG AAGCAGCACGCGGAGAAGTACTTCCCGCTGGACGCGGCGGCGAACGAGAAGGGCCTCCTGCTCAGCTTCGAGGA- CCGCACGGGGAAG CCCTGGCGCTTCCGCTACTCCTACTGGAACAGTAGCCAGAGCTACGTGATGACCAAGGGCTGGAGCCGCTTCGT- CAAGGAGAAGCGC CTCGACGCCGGGGACACAGTCTCCTTCGGCCGCGGCATCAGCGAGGCGGCGCGCGACAGGCTTTTCATCGACTG- GCGGTGCCGACCC GACCCGCCCGTCGTGCACCACCAGTACCACCACCGCCTCCCTCTCCCCTCCGCCGTCGTCCCCTACGCGCCGTG- GGCGGCGCACGCG CACCACCACCACTACCCAGCAGATGGGCACACGGAACCAGTAACACCTTGCCTGTGCGCCACACTCGTTGCCAC- TGAAATGAGAGCA TCATCTTCGCAACTGTCACTCACACGCTCCAACCTCTCCAGGCCGCCACAACCTAGAATAGCCAGAGTCGATGG- CGCCCAGCCACGG CCGTCGTCGTCACCACGCCAGCCACAGTCGTTGTGGTGCCGGTCGTGCCAACCGCAACCACGGCGAACGGCCGA- CGTTCCTTGA GRMZM2G082227_T01 Cover 45% identity 64% SEQ ID NO: 129 MEFTAPPPATRSGGGEERAAAEHHQQQQQATVEKEHMFDKVVTPSDVGKLNRLVIPKQHAERYFPLDAAANDKG- LLLSFEDRAGKPW RFRYSYWNSSQSYVMTKGWSRFVKEKRLDAGDTVSFGRGVGEAARGRLFIDWRRRPDPPVVHHQYHHHRLPLPS- AVVPYAPWAAAAH AHHHHYPAAGVGAARTTTTTTTTVLHHLPPSPSPLYLDTRRRHVGYDAYGAGTRQLLFYRPHQQPSTTVMLDSV- PVRLPPTPGQHAE PPPPAVASSASKRVRLFGVNLDCAAAAGSEEENVGGWRTSAPPTQQASSSSSYSSGKARCSLNLDL CDS SEQ ID NO: 130 ATGGAGTTCACCGCTCCCCCGCCCGCGACCCGGTCGGGCGGCGGCGAGGAGAGGGCGGCTGCTGAGCACCACCA- GCAGCAGCAGCAG GCGACGGTGGAGAAGGAGCACATGTTCGACAAGGTGGTGACGCCGAGCGACGTCGGGAAGCTGAACCGGCTGGT- GATCCCGAAGCAG CACGCGGAGAGGTACTTCCCGCTGGACGCGGCGGCGAACGACAAGGGCCTGCTGCTCAGCTTCGAGGACCGCGC- GGGGAAGCCCTGG CGCTTCCGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTGATGACCAAGGGCTGGAGCCGCTTCGTCAAGGA- GAAGCGCCTCGAC GCCGGGGACACCGTCTCCTTCGGCCGCGGCGTCGGCGAGGCGGCGCGCGGCAGGCTCTTCATCGACTGGCGGCG- CCGACCCGACCCG CCCGTCGTGCACCACCAGTACCACCACCACCGCCTCCCTCTCCCCTCCGCCGTCGTCCCCTACGCGCCGTGGGC- GGCGGCGGCGCAC GCGCACCACCACCACTACCCAGCAGCTGGGGTCGGTGCCGCCAGGACGACGACGACGACGACGACGACGGTGCT- CCACCACCTGCCG CCCTCGCCCTCCCCGCTCTACCTTGACACCCGCCGCCGCCACGTCGGCTACGACGCCTACGGGGCCGGCACCAG- GCAACTTCTCTTC TACAGGCCGCACCAGCAGCCCTCCACGACGGTGATGCTGGACTCCGTGCCGGTACGGTTACCGCCAACGCCAGG- GCAGCACGCCGAG CCGCCGCCCCCCGCCGTGGCGTCGTCAGCCTCGAAGCGGGTGCGCCTGTTCGGGGTGAACCTCGACTGCGCCGC- CGCCGCCGGCTCA GAGGAGGAGAACGTCGGCGGGTGGAGGACTAGTGCGCCGCCGACGCAGCAGGCGTCCTCCTCCTCATCCTACTC- TTCCGGGAAAGCG AGGTGCTCCTTGAACCTTGACTTGTGA GRMZM2G024948_T01 Cover 46% identity 63% SEQ ID NO: 131 MDQFAASGRFSREEEADEEQEDASNSMREISFMPPAAASSSSAAASASASASTSASACASGSSSAPFRSASASG- DAAGASGSGGPAD ADAEAEAVEKEHMFDKVVTPSDVGKLNRLVIPKQYAEKYFPLDAAANEKGLLLSFEDSAGKHWRFRYSYWNSSQ- SYVMTKGWSRFVK EKRLVAGDTVSFSRAAAEDARHRLFIDWKRRVDTRGPLRFSGLALPMPLPSSHYGGPHHYSPWGFGGGGGGGGG- FFMPPSPPATLYE HRLRQGLDFRSMTTTYPAPTVGRQLLFFGSARMPPHHAPPPQPRPFSLPLHHYTVQPSAAGVTAASRPVLLDSV- PVIESPTTAAKRV RLFGVNLDNNPDGGGEASHQGDALSLQMPGWQQRTPTLRLLELPRHGGESSAASSPSSSSSSKREARSALDLDL CDS SEQ ID NO: 132 ATGGACCAGTTCGCCGCGAGCGGGAGGTTCTCTAGAGAGGAGGAGGCGGACGAGGAGCAGGAGGATGCGTCCAA- TTCCATGCGCGAG ATCTCCTTCATGCCGCCGGCTGCGGCCTCGTCATCTTCGGCGGCTGCTTCCGCGTCCGCGTCCGCCTCCACCAG- CGCATCCGCGTGT GCATCGGGAAGCAGCAGCGCCCCCTTCCGCTCCGCCTCCGCGTCGGGGGATGCCGCCGGAGCGTCGGGGAGCGG- CGGCCCAGCGGAC GCGGACGCGGAGGCGGAGGCGGTGGAGAAGGAGCACATGTTCGACAAGGTGGTCACGCCGAGCGACGTGGGGAA- GCTCAACCGGCTG GTGATCCCGAAGCAGTACGCGGAGAAGTACTTCCCGCTGGACGCGGCGGCCAACGAGAAGGGCCTCCTCCTCAG- CTTCGAGGACAGC GCCGGCAAGCACTGGCGCTTCCGCTACTCCTACTGGAACAGCAGCCAGAGCTACGTCATGACCAAGGGCTGGAG- CCGCTTCGTCAAG GAGAAGCGCCTCGTCGCCGGGGACACCGTCTCCTTCTCCCGCGCCGCCGCCGAGGACGCGCGCCACCGCCTCTT- CATCGACTGGAAG CGCCGGGTCGACACCCGCGGCCCGCTTCGTTTCTCCGGCCTCGCGCTGCCGATGCCGCTGCCGTCGTCGCACTA- CGGCGGGCCCCAC CACTACAGCCCGTGGGGCTTCGGCGGCGGCGGCGGCGGCGGCGGCGGATTCTTCATGCCGCCCTCGCCGCCCGC- CACGCTCTACGAG CACCGCCTCAGACAGGGCCTCGACTTCCGCAGCATGACGACGACCTACCCCGCGCCGACCGTGGGGAGGCAGCT- CCTGTTTTTCGGC TCGGCCAGGATGCCTCCTCATCACGCGCCGCCGCCCCAGCCGCGCCCGTTCTCGCTGCCGCTGCATCACTACAC- GGTGCAACCGAGC GCCGCCGGCGTCACCGCCGCGTCACGGCCGGTCCTTCTTGACTCGGTGCCGGTCATCGAGAGCCCGACGACCGC- CGCGAAGCGCGTG CGGCTGTTCGGCGTCAACCTGGACAACAACCCAGATGGCGGCGGCGAGGCTAGCCATCAGGGCGATGCATTGTC- ATTGCAGATGCCC GGGTGGCAGCAAAGGACTCCAACTCTAAGGCTACTAGAATTGCCTCGCCATGGCGGGGAGTCCTCCGCGGCGTC- GTCTCCGTCGTCG TCGTCTTCCTCCAAGAGGGAGGCGCGTTCAGCTTTGGATCTCGATCTGTGA GRMZM2G328742_T01 Cover 55% identity 64% SEQ ID NO: 134 MATNHLSQGQHQHPQAWPWGVAMYTNLHYHHQQHHHYEKEHLFEKPLTPSDVGKLNRLVIPKQHAERYFPLSSS- GAGDKGLILCFED DDDDEAAAANKPWRFRYSYWTSSQSYVLTKGWSRYVKEKQLDAGDVVRFQRMRGFGMPDRLFISHSRRGETTAT- AATTVPPAAAAVR VVVAPAQSAGADHQQQQQPSPWSPMCYSTSGSYSYPTSSPANSQHAYHRHSADHDHSNNMQHAGESQSDRDNRS- CSAASAPPPPSRR LRLFGVNLDCGPGPEPETPTAMYGYMHQSPYAYNNWGSPYQHDEEI CDS 135 ATGGCCACGAACCATCTCTCCCAAGGGCAGCACCAGCACCCGCAGGCCTGGCCCTGGGGCGTGGCCATGTACAC- CAACCTACACTAC CACCACCAGCAGCACCACCACTACGAGAAGGAGCACCTGTTCGAGAAGCCGCTGACGCCGAGCGACGTGGGCAA- GCTCAACAGGCTG GTGATCCCCAAGCAGCACGCCGAGAGGTACTTCCCTCTCAGCAGCAGCGGCGCCGGCGACAAAGGCCTCATCCT- GTGCTTCGAGGAC GACGACGACGACGAGGCTGCCGCCGCCAACAAGCCGTGGCGGTTCCGCTACTCGTACTGGACCAGCAGCCAGAG- CTACGTGCTCACC AAGGGCTGGAGCCGCTACGTCAAGGAGAAGCAGCTTGACGCCGGCGACGTCGTGCGCTTCCAGAGGATGCGTGG- TTTCGGCATGCCC GACCGCCTGTTCATCAGCCACAGCCGCCGCGGCGAGACTACTGCTACTGCTGCAACAACAGTGCCCCCCGCTGC- TGCTGCCGTGCGC GTAGTAGTGGCACCTGCACAGAGCGCTGGCGCAGACCACCAGCAGCAGCAGCAGCCGTCGCCTTGGAGCCCAAT- GTGCTACAGCACA TCAGGCTCGTACTCGTACCCCACCAGCAGCCCAGCCAATTCCCAGCATGCCTACCACCGCCACTCAGCTGACCA- TGACCACAGCAAC AACATGCAACATGCAGGAGAATCTCAGTCCGACAGAGACAACAGGAGCTGCAGTGCAGCTTCGGCACCGCCGCC- ACCGTCGCGGCGG CTCCGGCTGTTCGGCGTAAACCTCGACTGCGGCCCGGGGCCGGAGCCGGAGACACCAACGGCGATGTACGGCTA- CATGCACCAAAGC CCCTACGCTTACAACAACTGGGGCAGTCCATACCAGCATGACGAGGAGATTTAA GRMZM2G142999_T01 Cover 44% identity 64% SEQ ID NO: 136 MEFTPAHAHARVVEDSERPRGGVAWVEKEHMFEKVVTPSDVGKLNRLVIPKQHAERYFPALDASSAAAAAAAAA- AGGGKGLVLSFED RAGKAWRFRYSYWNSSQSYVMTKGWSRFVKEKRLGAGDTVLFARGAGGARGRFFIDFRRRRQDLAFLQPTLASA- QRLLPLPSVPICP WQDYGASAPAPNRHVLFLRPQVPAAVVLKSVPVHVAASAVEATMSKRVRLFGVNLDCPPDAEDSATVPRGRAAS- TTLLQLPSPSSST SSSTAGKDVCCLDLGL CDS SEQ ID NO: 137 ATGGAGTTCACGCCCGCGCATGCGCATGCCCGTGTCGTTGAGGATTCCGAGAGGCCTCGCGGCGGCGTGGCCTG- GGTGGAGAAGGAG CACATGTTCGAGAAGGTGGTCACCCCGAGCGACGTGGGGAAGCTCAATCGCCTGGTCATCCCAAAGCAGCACGC- GGAGCGCTACTTC CCCGCGCTGGACGCCTCGTCCGCCGCGGCGGCGGCGGCGGCAGCAGCCGCGGGAGGCGGGAAGGGGCTGGTGCT- CAGCTTCGAGGAC CGGGCGGGGAAGGCGTGGCGCTTCCGCTACTCGTACTGGAACAGCAGCCAGAGCTACGTGATGACCAAAGGTTG- GAGCCGCTTCGTG AAGGAGAAGCGCCTCGGTGCCGGGGACACAGTCTTGTTCGCGCGCGGCGCGGGCGGCGCGCGCGGCCGCTTCTT- CATCGATTTCCGC CGCCGTCGCCAGGATCTCGCGTTCCTGCAGCCGACGCTGGCGTCTGCGCAGCGACTCCTGCCGCTGCCGTCGGT- GCCCATCTGCCCG TGGCAGGACTACGGCGCCTCGGCTCCGGCGCCCAACCGGCACGTGCTGTTCCTGCGGCCGCAGGTGCCGGCCGC- CGTAGTGCTCAAG TCGGTCCCCGTGCACGTTGCTGCATCCGCGGTGGAGGCGACCATGTCGAAGCGCGTCCGCCTGTTCGGGGTGAA- CCTCGACTGCCCG CCGGACGCCGAAGACAGCGCCACAGTCCCCCGGGGCCGGGCGGCGTCGACGACGCTTCTGCAACTGCCCTCGCC- ATCGTCGTCAACA TCCTCCTCGACGGCAGGGAAGGACGTGTGCTGTTTGGATCTTGGACTGTGA GRMZM2G125095_T01 Cover 85% identity 40% SEQ ID NO: 138 MEFRPAHARVFEDSERPRGGVAWLEKEHMFEKVVTPSDVGKLNRLVIPKQHAERYFPALDASAAAASASASAGG- GKAGLVLSFEDRA GKAWRFRYSYWNSSQSYVMTKGWSRFVKEKRLGAGDTVLFARGAGATRGRFFIDFRRRRHELAFLQPPLASAQR- LLPLPSVPICPWQ GYGASAPAPSRHVLFLRPQVPAAVVLTSVPVRVAASAVEEATRSKRVRLFGVNLDCPPDAEDGATATRTPSTLL- QLPSPSSSTSSST GGKDVRSLDLGL CDS SEQ ID NO: 139 ATGGAGTTCAGGCCCGCGCATGCCCGTGTCTTCGAGGATTCCGAGAGGCCTCGCGGCGGCGTGGCGTGGCTGGA- GAAGGAGCACATG TTCGAGAAAGTGGTCACCCCGAGCGACGTGGGGAAGCTCAATCGCCTGGTCATCCCGAAGCAGCACGCCGAGCG- CTACTTCCCCGCG CTGGACGCCTCGGCCGCCGCGGCGTCGGCATCGGCGTCGGCGGGCGGCGGGAAGGCGGGGCTGGTGCTCAGCTT- CGAGGACCGGGCG GGGAAGGCGTGGCGCTTCCGCTACTCGTACTGGAACAGCAGCCAGAGCTACGTGATGACCAAGGGATGGAGCCG- CTTCGTGAAAGAG AAGCGCCTCGGTGCCGGGGACACGGTATTGTTCGCGCGCGGCGCGGGCGCCACGCGCGGCCGCTTCTTCATCGA- TTTCCGCCGCCGC CGCCACGAGCTCGCGTTCCTGCAGCCGCCGCTGGCGTCTGCGCAGCGCCTCCTGCCGCTCCCGTCGGTGCCCAT- CTGCCCGTGGCAG GGCTACGGCGCCTCCGCTCCGGCGCCAAGCCGGCACGTGCTGTTCCTGCGGCCGCAGGTGCCGGCCGCCGTAGT- GCTCACGTCGGTG CCCGTGCGCGTCGCCGCATCCGCGGTGGAGGAGGCGACGAGGTCGAAGCGCGTCCGCCTGTTCGGGGTGAACCT- CGACTGCCCGCCG GACGCCGAAGACGGTGCCACAGCCACCCGGACGCCGTCGACGCTTCTGCAGCTGCCCTCGCCATCGTCGTCAAC- ATCCTCCTCCACG GGAGGCAAGGATGTGCGTTCTTTGGATCTTGGACTTTGA Tricum aeseirum TRAES3BF098300010CFD_ t1 Cover: 42% ident 60% SEQ ID NO: 140 MGVEILSSMVEHSFQYSSGVSTATTESGTAGTPPRPLSLPVAIADESVTSRSASSRFKGVVPQPNGRWGAQIYE- RHARVWLGTFPDQ DSAARAYDVASLRYRGRDVAFNFPCAAVEGELAFLAAHSKAEIVDMLRKQTYADELRQGLRRGRGMGARAQPTP- SWAREPLFEKAVT PSDVGKLNRLVVPKQHAEKHFPLKRTPETPTTTGKGVLLNFEDGEGKVWRFRYSYWNSSQSYVLTKGWSRFVRE- KGLGAGDSILFSC SLYEQEKQFFIDCKKNTSMNGGKSASPLPVGVTTKGEQVRVVRLFGVDISGVKRGRAATATAEQGLQELFKRQC- VAPGQHSPALGAF AL CDS SEQ ID NO: 141 ATGGGGGTGGAAATCCTGAGCTCCATGGTGGAGCACTCCTTCCAGTACTCTTCCGGCGTGTCCACGGCCACGAC- GGAGTCAGGCACC GCCGGAACACCGCCGAGGCCTTTGAGCCTACCTGTCGCCATCGCCGACGAGTCCGTGACCTCGCGGTCGGCGTC- GTCTCGGTTCAAG GGCGTGGTGCCGCAGCCAAACGGGCGATGGGGCGCCCAGATCTACGAGCGCCACGCTCGCGTCTGGCTCGGCAC- GTTCCCAGACCAG GACTCGGCGGCGCGCGCCTACGACGTAGCCTCGCTCAGGTACCGCGGCCGCGACGTCGCCTTCAACTTCCCGTG- CGCGGCCGTGGAG GGGGAGCTCGCCTTCCTGGCGGCGCACTCCAAGGCTGAGATAGTGGACATGCTCCGGAAGCAGACCTACGCCGA- TGAACTCCGCCAG GGCCTGCGGCGCGGCCGTGGCATGGGGGCGCGCGCGCAGCCGACGCCGTCGTGGGCGCGGGAGCCCCTTTTCGA- GAAGGCCGTGACC CCTAGCGATGTCGGCAAGCTCAATCGCCTCGTAGTGCCGAAGCAGCACGCCGAGAAGCACTTCCCCCTGAAGCG- CACGCCGGAGACG CCGACCACCACCGGCAAGGGCGTGCTGCTCAACTTCGAGGACGGCGAGGGGAAGGTGTGGAGGTTCCGGTACTC- GTACTGGAACAGC AGCCAGAGCTACGTGCTCACCAAAGGCTGGAGCCGCTTCGTCCGGGAGAAGGGCCTAGGTGCCGGCGACTCCAT- CCTATTCTCGTGC TCGCTGTACGAACAGGAGAAGCAGTTCTTCATCGACTGCAAGAAGAACACTAGCATGAACGGAGGCAAATCGGC- GTCGCCGCTGCCA GTGGGGGTGACTACCAAAGGAGAACAAGTTCGCGTCGTTAGGCTATTCGGTGTCGACATCTCGGGAGTGAAGAG- GGGGCGAGCGGCG ACGGCAACGGCGGAGCAAGGCCTGCAGGAGTTGTTCAAGAGGCAATGCGTGGCACCCGGCCAGCACTCTCCTGC- CCTAGGTGCCTTC

GCCTTATAG TRAES3BF062700040CFD_t1 Cover 47% ident 55% SEQ ID NO: 142 MASGKPTNHGMEDDNDMEYSSAESGAEDAAEPSSSPVLAPPRAAPSSRFKGVVPQPNGRWGAQIYEKHSRVWLG- TFPDEDAAVRAYD VAALRFRGPDAVINHQRPTAAEEAGSSSSRSELDPELGFLADHSKAEIVDMLRKHTYDDELRQGLRRGRGRAQP- TPAWARELLFEKA VTPSDVGKLNRLVVPKQQAEKHFPPTTAAATGSNGKGVLLNFEDGEGKVWRFRYSYWNSSQSYVLTKGWSRFVK- ETGLRAGDTVAFY RSAYGNDTEDQLFIDYKKMNKNDDAADAAISDENETGHVAVKLFGVDIAGGGMAGSSGG CDS SEQ ID NO: 143 ATGGCATCTGGCAAGCCGACAAACCACGGGATGGAGGACGACAACGACATGGAGTACTCCTCCGCGGAATCGGG- GGCCGAGGACGCG GCGGAGCCGTCGTCGTCGCCGGTGCTGGCGCCGCCCCGGGCGGCTCCATCGTCGCGGTTCAAGGGCGTCGTGCC- GCAGCCCAACGGG CGGTGGGGAGCGCAGATCTACGAGAAGCACTCGCGGGTGTGGCTCGGAACGTTCCCCGACGAGGACGCCGCCGT- GCGCGCCTACGAC GTGGCCGCGCTCCGCTTCCGCGGCCCGGACGCCGTCATCAACCACCAGCGACCGACGGCCGCGGAGGAGGCCGG- CTCGTCGTCGTCC AGGAGCGAGCTGGATCCAGAGCTCGGCTTCCTTGCCGACCACTCCAAGGCCGAGATCGTCGACATGCTCCGGAA- GCACACCTACGAC GACGAGCTCCGTCAGGGCCTGCGCCGCGGCCGCGGGCGCGCGCAGCCGACGCCGGCGTGGGCACGAGAGCTCCT- CTTCGAGAAGGCC GTGACCCCGAGCGACGTCGGCAAGCTCAACCGCCTCGTGGTGCCGAAGCAGCAGGCCGAGAAGCACTTCCCTCC- GACCACTGCGGCG GCCACCGGCAGCAACGGCAAGGGCGTGCTGCTCAACTTCGAGGACGGCGAAGGGAAGGTGTGGCGCTTCCGGTA- CTCGTACTGGAAC AGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTTCGTCAAGGAGACGGGCCTCCGCGCCGGCGACAC- CGTGGCGTTCTAC CGGTCGGCGTACGGGAATGACACGGAGGATCAGCTCTTCATCGACTACAAGAAGATGAACAAGAATGACGATGC- TGCGGACGCGGCG ATTTCCGATGAGAATGAGACAGGCCATGTCGCCGTCAAGCTCTTCGGCGTTGACATTGCCGGTGGAGGGATGGC- GGGATCATCAGGT GGCTGA TRAES3BF062600010CFD_t1 Cover 43% ident 58% SEQ ID NO: 144 MASGKPTNHGMEDDNDMEYSSAESGAEDAAEPSSSPVLAPPRAAPSSRFKGVVPQPNGRWGAQIYEKHSRVWLG- TFPDEDAAARAYD VAALRFRGPDAVINHQRPTAAEEAGSSSSRSELDPELGFLADHSKAEIVDMLRKHTYDDELRQGLRRGRGRAQP- TPAWARELLFEKA VTPSDVGKLNRLVVPKQQAEKHFPPTTAAATGSNGKGVLLNFEDGEGKVWRFRYSYWNSSQSYVLTKGWSRFVK- ETGLRAGDTVAFY RSAYGNDTEDQLFIDYKKMNKNDDAADAAISDENETGHVAVKLFGVDIAGGGMAGSSGG CDS SEQ ID NO: 145 ATGGCATCTGGCAAGCCGACAAACCACGGGATGGAGGACGACAACGACATGGAGTACTCCTCCGCGGAATCGGG- GGCCGAGGACGCG GCGGAGCCGTCGTCGTCGCCGGTGCTGGCGCCGCCCCGGGCGGCTCCATCGTCGCGGTTCAAGGGCGTCGTGCC- GCAGCCCAACGGG CGGTGGGGAGCGCAGATCTACGAGAAGCACTCGCGGGTGTGGCTCGGAACGTTCCCCGACGAGGACGCCGCCGC- GCGCGCCTACGAC GTGGCCGCGCTCCGCTTCCGCGGCCCGGACGCCGTCATCAACCACCAGCGACCGACGGCCGCGGAGGAGGCCGG- CTCGTCGTCGTCC AGGAGCGAGCTGGATCCAGAGCTCGGCTTCCTCGCCGACCACTCCAAGGCCGAGATCGTCGACATGCTCCGGAA- GCACACCTACGAC GACGAGCTCCGTCAGGGCCTGCGCCGCGGCCGCGGGCGCGCGCAGCCGACGCCGGCGTGGGCACGAGAGCTCCT- CTTCGAGAAGGCC GTGACCCCGAGCGACGTCGGCAAGCTCAACCGCCTCGTGGTGCCGAAGCAGCAGGCCGAGAAGCACTTCCCTCC- GACCACTGCGGCG GCCACCGGCAGCAACGGCAAGGGCGTGCTGCTCAACTTCGAGGACGGCGAAGGGAAGGTGTGGCGCTTCCGGTA- CTCGTACTGGAAC AGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTTCGTCAAGGAGACGGGCCTCCGCGCCGGCGACAC- CGTGGCGTTCTAC CGGTCGGCGTACGGGAATGACACGGAGGATCAGCTCTTCATCGACTACAAGAAGATGAACAAGAATGACGATGC- TGCGGACGCGGCG ATTTCCGATGAGAATGAGACAGGCCATGTCGCCGTCAAGCTCTTCGGCGTTGACATTGCCGGTGGAGGGATGGC- GGGATCATCAGGT GGCTGA

Sequence CWU 1

1

2721804DNAArtificial SequencecDNA 1atgtcagtca accattacca caacactctc tcgttgcatc atcaccacca aaacgacgta 60gctatagcac aacgagagtc tttgttcgag aaatcactca caccaagcga cgtcggaaag 120ctaaaccgct tagtcatacc aaaacaacac gccgagaaat acttccctct caataataat 180aataataatg gcggcagcgg agatgacgtg gcgacgacgg agaaagggat gcttcttagc 240ttcgaggatg agtcaggcaa gtgttggaaa ttcagatact cttattggaa cagtagccaa 300agctacgtgt tgaccaaagg atggagcagg tacgtcaaag acaaacacct cgacgcaggc 360gacgttgttt tctttcaacg tcaccgtttt gatctccata gactcttcat tggctggcgg 420agacgcggtg aagcttcttc ctctcccgct gtctccgttg tgtctcaaga agctctagtt 480aatacgacgg cgtattggag cggcttgact acaccttatc gtcaagtaca cgcgtcaact 540acttacccta atattcacca agagtattca cactatggcg ccgtcgttga tcatgctcag 600tcgataccac cggtggtcgc aggtagctcg aggacggtga ggctttttgg cgtgaacctc 660gaatgtcatg gtgatgccgt cgagccacca ccgcgtcctg atgtctataa tgaccaacac 720atttactatt actcaactcc tcatcccatg aatatatcat ttgctgggga agcattggag 780caggtaggag atggacgagg ttga 80424285DNAArabidopsis thaliana 2ttgtttcggc tatttgttat actattgtta taacagtcac aagacttgac ctcaacgaaa 60acttttacaa aacgtgaatt ggaaattttt acaaaatatg ctcttaatcg ttaatgcttc 120ccaattaggt gagttaaatt gtgagaggaa ccatttctta gaggaaatgg ttcatgaaaa 180caaatatgaa atagtatcac tagtcttagt tttgcgagaa aattaggaaa aatagaaacg 240tgtaagcacc aatgatattc ctgaaagcac gtgacagata tttcatgatc ctataattaa 300caagtgataa agatattaaa taaaattaac gatacttgag aaattcgtca aataaaatag 360aagaggacca ctcacgtaac catttgcacg tcccattgat ttttgtggta gacttggtat 420gttatattac ttatattcac agaattatat acgaaactca cgacttaaga tgcacggtaa 480taactacaga tggaaattta cccatcaaac aagaaaacaa catttactca agcatctagc 540tagaccaaaa tgtttgttta cttgttgact tgcgatccat agatatatta gttagaactt 600tttcttctac aattgatcaa atgtttcaca ctgttctcaa tttctcatct agattcatga 660cttatatgtt tggtcaaata tcacagcttg atgagcatta aatagcgtcg aagtatagga 720tggttacgtt gttcaatatt gtaaaggaaa aaaagagaaa gagtgccaaa aggtcaagtc 780gatttcacaa ataaatcttg aagtctttat ccctctcgat tataaaatga ttaggaaaag 840aaaaagagag aataaaatgt agataaagag aaagagaaag agagagagga acataaggga 900tggtatgaag tagaagtgaa gatgcatgcg atggtgtgtc ggaaaggcaa agcacatgct 960acacaacttg agcttctcac ttgcgtcagg gataagtatc ctctgtacct tcttactttt 1020gcgtaatatg taccacctca cttctcaacc gtttgatctt taatccttca ttatttcttc 1080attaccttct ctttttgttt ttgttttcgt tttcaatttc tcatagattc atttacaaac 1140taaatatcat aggaaggtgt tatctctagt taatttctta tcctacttta acaaaattta 1200attgtcaaaa gattattttt acgtttatag acaaaagata ctgacacatc aattccacga 1260accaaatggt tgagaaaaac aaaacgacta tctttgtctt gcaaataaat taatggcagt 1320tagtaagatt ctcagctgaa aattcataca agagtaaatg atcaaataac catttatgag 1380agaaatttaa tccttcagaa accaatgagg atctgatcaa gtaattgcaa accacatgag 1440tccatgataa aggattgttt gacttacgca atccacatat ttatggctgc ttgatatgta 1500aggtttatct gctttgacag tctatagaat cttgctaatc aatacgtcat atccggtgaa 1560tactgaaact tttttaatta agaaaacaca aatcatcttt tctccggagg atttcgaatt 1620tagttccggc aatgctgaaa taacatatgt tgaacttata acattccaag acatcaaatt 1680ttactaatat ataaataatt acatattctt cttctacatg atcaaaacct tttcaacttt 1740aattaaaggg ttacgtcgcg gcgttttgtg tggcttactc tttttttaca ctataactat 1800agaacactcg tggatccaat gccgtttagg acaagatttt atcagacgag aaaaaaaaaa 1860acaataccac atttttaaat atatatggat tatggactgc aacaacaata tagaaaagaa 1920gagaaaaaaa taaaaataat gattgaaagg aaatatcatc acgcaaaacc ttaaaagtac 1980tatcggtatc gtgtcgtcct ctcctcatca aatagttccc acagttttca catcaattta 2040accattttca atttttttca ctctctgtct ctctcctttg tataatacta tattagtacc 2100attacccatc tctctttcac caccaaacca acacctgcaa atcctctctc tctctctcac 2160tccaagaaac caaaaaaaaa gatgtcagtc aaccattacc acaacactct ctcgttgcat 2220catcaccacc aaaacgacgt agctatagca caacgagagt ctttgttcga gaaatcactc 2280acaccaagcg acgtcggaaa gctaaaccgc ttagtcatac caaaacaaca cgccgagaaa 2340tacttccctc tcaataataa taataataat ggcggcagcg gagatgacgt ggcgacgacg 2400gagaaaggga tgcttcttag cttcgaggat gagtcaggca agtgttggaa attcagatac 2460tcttattgga acagtagcca aagctacgtg ttgaccaaag gatggagcag gtacgtcaaa 2520gacaaacacc tcgacgcagg cgacgttgtt ttctttcaac gtcaccgttt tgatctccat 2580agactcttca ttggctggcg gagacgcggt gaagcttctt cctctcccgc tgtctccgtt 2640gtgtctcaag aagctctagt taatacgacg gcgtattgga gcggcttgac tacaccttat 2700cgtcaagtac acgcgtcaac tacttaccct aatattcacc aagagtattc acactatggt 2760aaattcaaac cctttatttc ctcttttgtt ttttctttct ctcttatcta tatgtcagat 2820ttatactcct ctctgttctc ttttaagatt tgtctttttc ataaaaatag atgattcgta 2880atttgtattg catatttaca tgttctctta aaaaaagtaa tagagattaa tattttatgc 2940atggtatttt agattatctg cctactttat atggtagtaa acaagaacat tcatctttat 3000ttggttttat aaacaaaata tgagaatttt taaaggttag ggcaagcact tggaaagctc 3060aaccatttta gttagctggt ggaatatctt tcttataaaa agcaaatgag ttatctaaaa 3120ctatatgaca attattttag ttgcgtgtgt aatgtatata aaataacaac atgaaataac 3180attttgtctt ttatttttgt cattcttatt atttaatttt ggacccgaca atttcaaata 3240atcttctcca agttgtaact aatccgttac atgcgcgtga ggagaaccgt ccaatccact 3300tagactaacg tgccctttat ttcttccttt taattctatg ttaaaaaaac aatttaacta 3360aaagatgcgc acgtgtcttg acggtggaaa aaaattgtag gcgccgtcgt tgatcatgct 3420cagtcgatac caccggtggt cgcaggtagc tcgaggacgg tgaggctttt tggcgtgaac 3480ctcgaatgtc atggtgatgc cgtcgagcca ccaccgcgtc ctgatgtcta taatgaccaa 3540cacatttact attactcaac tcctcatccc atggtaaata tttttttttt ttacattttt 3600gtcagattca aatttttgct tacgtatgat ataattatta aacagatgtc gtggctgttt 3660ctcgagacga gacagatgaa aattagtaat tttaaaatag acctgaaaga gatttttatg 3720tttaataaat tatataaagg aggaatcaga gagaataata ctatacactt gactgtaaaa 3780ccacatggcc aatttggttt ttatttgatt actttgattt gttttgttta ctcttttgtc 3840tctgtagcct ccttttgttc attaattaat atcagccgta agtatatagt ttcctgtgaa 3900aacagtctct attttggttt tactattcta atttgttagg caccgtcagt tttttttgtg 3960aaaccaaatt attgactaat aagctggaaa gcaaaactga ctaaaagcat tacaaactta 4020tcaatgacat aagttttgaa tttattacca tgttttgtaa tgttcagata taatttgaaa 4080tgcttagaat tatatatttg tatacttaaa ttaatgaaat aaagtgaata ctaaagatag 4140ttttattttt catattattc tatacaattc ggtgtacaat ttgtttttga tgataataaa 4200aataataaaa ttgcgtgttg gaattgtgaa acagaatata tcatttgctg gggaagcatt 4260ggagcaggta ggagatggac gaggt 42853267PRTArabidopsis thaliana 3Met Ser Val Asn His Tyr His Asn Thr Leu Ser Leu His His His His1 5 10 15Gln Asn Asp Val Ala Ile Ala Gln Arg Glu Ser Leu Phe Glu Lys Ser 20 25 30Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys 35 40 45Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Asn Asn Asn Asn Asn Gly 50 55 60Gly Ser Gly Asp Asp Val Ala Thr Thr Glu Lys Gly Met Leu Leu Ser65 70 75 80Phe Glu Asp Glu Ser Gly Lys Cys Trp Lys Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val 100 105 110Lys Asp Lys His Leu Asp Ala Gly Asp Val Val Phe Phe Gln Arg His 115 120 125Arg Phe Asp Leu His Arg Leu Phe Ile Gly Trp Arg Arg Arg Gly Glu 130 135 140Ala Ser Ser Ser Pro Ala Val Ser Val Val Ser Gln Glu Ala Leu Val145 150 155 160Asn Thr Thr Ala Tyr Trp Ser Gly Leu Thr Thr Pro Tyr Arg Gln Val 165 170 175His Ala Ser Thr Thr Tyr Pro Asn Ile His Gln Glu Tyr Ser His Tyr 180 185 190Gly Ala Val Val Asp His Ala Gln Ser Ile Pro Pro Val Val Ala Gly 195 200 205Ser Ser Arg Thr Val Arg Leu Phe Gly Val Asn Leu Glu Cys His Gly 210 215 220Asp Ala Val Glu Pro Pro Pro Arg Pro Asp Val Tyr Asn Asp Gln His225 230 235 240Ile Tyr Tyr Tyr Ser Thr Pro His Pro Met Asn Ile Ser Phe Ala Gly 245 250 255Glu Ala Leu Glu Gln Val Gly Asp Gly Arg Gly 260 2654849DNAArtificial SequencecDNA 4atgtcagtca accattactc cacagaccac caccacactc tcttgtggca gcaacagcaa 60caccgccaca ccaccgacac atcggagaca accaccaccg ccacatggct ccacgacgac 120ctaaaagagt cactcttcga gaagtctctc acaccaagcg acgtcgggaa actcaaccgc 180ctcgtcatac caaaacaaca cgcagagaaa tacttccctc tcaatgccgt cctagtctcc 240tctgctgctg ctgacacgtc atcttcggag aaagggatgc ttctaagctt tgaagacgag 300tcaggcaagt catggaggtt cagatactct tactggaaca gcagtcaaag ctatgtcttg 360actaaaggat ggagcagatt tgtcaaagac aaacagctcg atccaggcga cgttgttttc 420ttccaacgac accgttctga ttctaggaga ctcttcattg gctggcgcag acgtggacaa 480ggctcctcat cctccgtcgc ggccactaac tccgccgtga atacgagttc tatgggagct 540ctttcttatc atcaaatcca cgccactagt aattactcta atcctccctc tcactcagag 600tattcccact atggagccgc cgtagcaaca gcggctgaga ctcacagcac accgtcgtct 660tccgtcgtcg ggagctcaag gacggtgagg cttttcggtg tgaatctgga gtgtcaaatg 720gatgaaaacg acggagatga ttctgttgca gttgccacca ccgttgaatc tcccgacggt 780tactacggcc aaaacatgta ctattattac tctcatcctc ataacatggt aattttaact 840cttttataa 8495282PRTArabidopsis thaliana 5Met Ser Val Asn His Tyr Ser Thr Asp His His His Thr Leu Leu Trp1 5 10 15Gln Gln Gln Gln His Arg His Thr Thr Asp Thr Ser Glu Thr Thr Thr 20 25 30Thr Ala Thr Trp Leu His Asp Asp Leu Lys Glu Ser Leu Phe Glu Lys 35 40 45Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 50 55 60Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Ala Val Leu Val Ser65 70 75 80Ser Ala Ala Ala Asp Thr Ser Ser Ser Glu Lys Gly Met Leu Leu Ser 85 90 95Phe Glu Asp Glu Ser Gly Lys Ser Trp Arg Phe Arg Tyr Ser Tyr Trp 100 105 110Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val 115 120 125Lys Asp Lys Gln Leu Asp Pro Gly Asp Val Val Phe Phe Gln Arg His 130 135 140Arg Ser Asp Ser Arg Arg Leu Phe Ile Gly Trp Arg Arg Arg Gly Gln145 150 155 160Gly Ser Ser Ser Ser Val Ala Ala Thr Asn Ser Ala Val Asn Thr Ser 165 170 175Ser Met Gly Ala Leu Ser Tyr His Gln Ile His Ala Thr Ser Asn Tyr 180 185 190Ser Asn Pro Pro Ser His Ser Glu Tyr Ser His Tyr Gly Ala Ala Val 195 200 205Ala Thr Ala Ala Glu Thr His Ser Thr Pro Ser Ser Ser Val Val Gly 210 215 220Ser Ser Arg Thr Val Arg Leu Phe Gly Val Asn Leu Glu Cys Gln Met225 230 235 240Asp Glu Asn Asp Gly Asp Asp Ser Val Ala Val Ala Thr Thr Val Glu 245 250 255Ser Pro Asp Gly Tyr Tyr Gly Gln Asn Met Tyr Tyr Tyr Tyr Ser His 260 265 270Pro His Asn Met Val Ile Leu Thr Leu Leu 275 280633PRTArtificial Sequencedomain 6Ser Asn Asn Asn Asn Asn Asn Gly Gly Ser Gly Asp Asp Val Ala Cys1 5 10 15His Phe Gln Arg Phe Asp Leu His Arg Leu Phe Ile Gly Trp Arg Gly 20 25 30Glu79PRTArtificial Sequencedomain 7Val Arg Leu Phe Gly Val Asn Leu Glu1 5821DNAArtificial Sequenceprimer 8accatgacat tcgaggttca c 21921DNAArtificial Sequenceprimer 9atcaccacca aaacgacgta g 211021DNAArtificial Sequenceprimer 10tacgtcatgc ttcaaatcgt g 211121DNAArtificial Sequenceprimer 11aggacacgaa caattcattc g 211229DNAArtificial Sequenceprimer 12tacgaataag agcgtccatt ttagagtga 291321DNAArtificial Sequenceprimer 13acccaaagaa cagcaatcat g 211421DNAArtificial Sequenceprimer 14aaaacactcc gccattaaac c 211524DNAArtificial Sequenceprimer 15cgagtatcaa tggaaactta accg 241620DNAArtificial Sequenceprimer 16aacggagagt ggcttgagat 201720DNAArtificial Sequenceprimer 17tggcccttat ggtttctgca 201815DNAArtificial Sequenceprimermisc_feature(1)..(1)n is a, c, g, or tmisc_feature(6)..(6)n is a, c, g, or tmisc_feature(8)..(8)n is a, c, g, or tmisc_feature(10)..(10)n is a, c, g, or tmisc_feature(12)..(12)n is a, c, g, or t 18ntcgantntn gngtt 151921DNAArtificial Sequenceprimer 19atgtcagtca accattacca c 212024DNAArtificial Sequenceprimer 20caggtaggag atggacgagg ttga 242123DNAArtificial Sequenceprimer 21tgagaggaac catttcttag agg 232222DNAArtificial Sequenceprimer 22acctcgtcca tctcctacct gc 222322DNAArtificial Sequenceprimer 23aaacacgtca aatataacga at 222433DNAArtificial Sequenceprimer 24cttttttttg gtttcttgga gtgagagaga gag 332529DNAArtificial Sequenceprimer 25agtctgggcc catgtcagtc aaccattac 292629DNAArtificial Sequenceprimer 26gcgactagtt tataaaagag ttaaaatta 292724DNAArtificial Sequenceprimer 27cgggatcctc agtcaaccat tacc 242828DNAArtificial Sequenceprimer 28actagtcgac tcaacctcgt ccatctcc 282920DNAArtificial Sequenceprimer 29gaaatcacag cacttgcacc 203020DNAArtificial Sequenceprimer 30aagcctttga tcttgagagc 203118DNAArtificial Sequenceprimer 31gcgacgacgg agaaaggg 183218DNAArtificial Sequenceprimer 32acgacggcgc catagtgt 183322DNAArtificial Sequenceprimer 33tttgaagacg agtcaggcaa gt 223420DNAArtificial Sequenceprimer 34tacggcggct ccatagtggg 203524DNAArtificial Sequenceprimer 35gtattggagc ggcttgacta cacc 243622DNAArtificial Sequenceprimer 36gacggcatca ccatgacatt cg 223724DNAArtificial Sequenceprimer 37tgattctgac atgattgctg ttct 243823DNAArtificial Sequenceprimer 38tcgcaactgt atctgtccct cta 233925DNAArtificial Sequenceprimer 39cgtttcgctt tccttagtgt tagct 254027DNAArtificial Sequenceprimer 40agcgaacgga tctagagact caccttg 274123DNAArtificial Sequenceprimer 41caggcctaag cctaacagta gac 234223DNAArtificial Sequenceprimer 42tgtactagga tttatttacg tag 234324DNAArtificial Sequenceprimer 43tattgttcat agaaaccctg caaa 244424DNAArtificial Sequenceprimer 44agtcaatggt ttaatggcgg agtg 244523DNAArtificial Sequenceprimer 45ttctactaca cttgctctct gta 234623DNAArtificial Sequenceprimer 46tacagagagc aagtgtagta gaa 234723DNAArtificial Sequenceprimer 47ttctactaac acctctctct gta 234823DNAArtificial Sequenceprimer 48tacagagaga ggtgttagta gaa 2349192PRTOryza sativa 49Met Ala Met His Ala Gly His Ala Trp Trp Gly Val Ala Met Tyr Thr1 5 10 15Asn His Tyr His His His Tyr Arg His Lys Thr Ser Asp Val Gly Lys 20 25 30Asn Arg Val Lys His Ala Arg Tyr Gly Gly Gly Asp Ser Gly Lys Gly 35 40 45Ser Asp Ser Gly Lys Trp Arg Arg Tyr Ser Tyr Trp Thr Ser Ser Ser 50 55 60Tyr Val Thr Lys Gly Trp Ser Arg Tyr Val Lys Lys Arg Asp Ala Gly65 70 75 80Asp Val Val His Arg Val Arg Gly Gly Ala Ala Asp Arg Gly Cys Arg 85 90 95Arg Arg Gly Ser Ala Ala Ala Val Arg Val Thr Ala Asn Gly Gly Trp 100 105 110Ser Met Cys Tyr Ser Thr Ser Gly Ser Ser Tyr Asp Thr Ser Ala Asn 115 120 125Ser Tyr Ala Tyr His Arg Ser Val Asp Asp His Ser Asp His Ala Gly 130 135 140Ser Arg Ala Asp Ala Lys Ser Ser Ser Ala Ala Ser Ala Ser Arg Arg145 150 155 160Arg Gly Val Asn Asp Cys Gly Ala Asp Ala Thr Ala Met Tyr Gly Tyr 165 170 175Met His His Ser Tyr Ala Ala Val Ser Thr Val Asn Tyr Trp Ser Val 180 185 19050834DNAOryza sativa 50atggccatgc accctctcgc ccaggggcac ccccaggcgt ggccatgggg tgtagccatg 60tacaccaacc tgcactacca ccaccactac gagagggagc acctgttcga gaagccgctg 120acgccgagcg acgtcggcaa

gctcaacagg ctggtgatcc ccaagcagca cgccgagagg 180tacttcccgc tcggcggcgg cgactccggt gagaagggcc tcctcctctc cttcgaggac 240gagtccggca agccatggcg gttccgctac tcctactgga ccagcagcca gagctacgtg 300ctcaccaagg gctggagccg ctacgtcaag gagaagcgcc tcgacgccgg cgacgtcgtc 360cacttcgagc gcgtccgcgg cctcggcgcc gccgaccgcc tcttcatcgg ctgcaggcgc 420cgcggcgaga gcgcgcccgc gccgccgccc gccgttcgcg tcacgccgca gccgcctgcc 480ctcaacggcg gcgagcagca gccgtggagc ccaatgtgtt acagcacgtc gggctcgtcc 540tacgacccta ccagccctgc caattcatat gcctaccatc gctccgtaga ccaagatcac 600agcgacatac tacacgcagg agagtcgcag agagaagcag acgccaagag cagcagcgcg 660gcgtcggcgc cgccgccgtc gaggcggctc aggctgttcg gcgttaacct cgactgcggc 720ccggagccgg aggcggatca ggcgacggca atgtacggct acatgcacca ccagagcccc 780tacgccgcag tgtctacagt gccaaattac tggtcagtat tttttcagtt ttaa 83451279PRTOryza sativa 51Met Ala Met Asn His Pro Leu Phe Ser Gln Glu Gln Pro Gln Ser Trp1 5 10 15Pro Trp Gly Val Ala Met Tyr Ala Asn Phe His Tyr His His His Tyr 20 25 30Glu Lys Glu His Met Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly 35 40 45Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 50 55 60Pro Leu Gly Ala Gly Asp Ala Ala Asp Lys Gly Leu Ile Leu Ser Phe65 70 75 80Glu Asp Glu Ala Gly Ala Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Thr 85 90 95Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys 100 105 110Glu Lys Arg Leu Asp Ala Gly Asp Val Val His Phe Glu Arg Val Arg 115 120 125Gly Ser Phe Gly Val Gly Asp Arg Leu Phe Ile Gly Cys Arg Arg Arg 130 135 140Gly Asp Ala Ala Ala Ala Gln Thr Pro Ala Pro Pro Pro Ala Val Arg145 150 155 160Val Ala Pro Ala Ala Gln Asn Ala Gly Glu Gln Gln Pro Trp Ser Pro 165 170 175Met Cys Tyr Ser Thr Ser Gly Gly Gly Ser Tyr Pro Thr Ser Pro Ala 180 185 190Asn Ser Tyr Ala Tyr Arg Arg Ala Ala Asp His Asp His Gly Asp Met 195 200 205His His Ala Asp Glu Ser Pro Arg Asp Thr Asp Ser Pro Ser Phe Ser 210 215 220Ala Gly Ser Ala Pro Ser Arg Arg Leu Arg Leu Phe Gly Val Asn Leu225 230 235 240Asp Cys Gly Pro Glu Pro Glu Ala Asp Thr Thr Ala Ala Ala Thr Met 245 250 255Tyr Gly Tyr Met His Gln Gln Ser Ser Tyr Ala Ala Met Ser Ala Val 260 265 270Pro Ser Tyr Trp Gly Asn Ser 27552840DNAOryza sativa 52atggccatga accaccctct cttctcccag gagcaacccc agtcctggcc atggggtgtg 60gccatgtacg ccaacttcca ctaccaccac cactacgaga aggagcacat gtttgagaag 120cccctgacgc ccagtgacgt ggggaagctg aaccggctgg tgatccccaa gcagcacgcc 180gagaggtact tccccctcgg cgccggcgac gccgccgaca agggcctgat cctgtcgttc 240gaggacgagg ccggcgcgcc gtggcggttc aggtactcct actggacgag cagccagagc 300tacgtgctca ccaagggctg gagccgctac gtcaaggaga agcgcctcga cgccggcgac 360gtcgtgcact tcgagagggt gcgcggctcc ttcggcgtcg gcgaccgtct cttcatcggc 420tgcaggcgcc gcggcgacgc cgccgccgcg caaacacccg caccgccgcc cgccgtgcgc 480gtcgccccgg ctgcacagaa cgccggcgag cagcagccgt ggagcccaat gtgttacagc 540acgtcgggcg gcggctcata ccctaccagc ccagccaact cctacgccta ccgccgcgca 600gcagatcatg atcacgggga catgcaccat gcagacgagt ctccgcgcga cacggacagc 660ccaagcttca gtgcaggctc ggcgccatcg aggcggctca ggctgttcgg cgtcaacctc 720gactgcgggc cagagccgga ggcagacacc acggcagcgg caacaatgta cggctacatg 780caccagcaga gctcctatgc tgccatgtct gcagtaccca gttactgggg caattcataa 84053412PRTOryza sativa 53Met Glu Phe Thr Thr Ser Ser Arg Phe Ser Lys Glu Glu Glu Asp Glu1 5 10 15Glu Gln Asp Glu Ala Gly Arg Arg Glu Ile Pro Phe Met Thr Ala Thr 20 25 30Ala Glu Ala Ala Pro Ala Pro Thr Ser Ser Ser Ser Ser Pro Ala His 35 40 45His Ala Ala Ser Ala Ser Ala Ser Ala Ser Ala Ser Gly Ser Ser Thr 50 55 60Pro Phe Arg Ser Asp Asp Gly Ala Gly Ala Ser Gly Ser Gly Gly Gly65 70 75 80Gly Gly Gly Gly Gly Glu Ala Glu Val Val Glu Lys Glu His Met Phe 85 90 95Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val 100 105 110Ile Pro Lys Gln Tyr Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala Ala 115 120 125Asn Glu Lys Gly Leu Leu Leu Asn Phe Glu Asp Arg Ala Gly Lys Pro 130 135 140Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met145 150 155 160Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly 165 170 175Asp Thr Val Ser Phe Ser Arg Gly Ile Gly Asp Glu Ala Ala Arg His 180 185 190Arg Leu Phe Ile Asp Trp Lys Arg Arg Ala Asp Thr Arg Asp Pro Leu 195 200 205Arg Leu Pro Arg Gly Leu Pro Leu Pro Met Pro Leu Thr Ser His Tyr 210 215 220Ala Pro Trp Gly Ile Gly Gly Gly Gly Gly Phe Phe Val Gln Pro Ser225 230 235 240Pro Pro Ala Thr Leu Tyr Glu His Arg Leu Arg Gln Gly Leu Asp Phe 245 250 255Arg Ala Phe Asn Pro Ala Ala Ala Met Gly Arg Gln Val Leu Leu Phe 260 265 270Gly Ser Ala Arg Ile Pro Pro Gln Ala Pro Leu Leu Ala Arg Ala Pro 275 280 285Ser Pro Leu His His His Tyr Thr Leu Gln Pro Ser Gly Asp Gly Val 290 295 300Arg Ala Ala Gly Ser Pro Val Val Leu Asp Ser Val Pro Val Ile Glu305 310 315 320Ser Pro Thr Thr Ala Ala Lys Arg Val Arg Leu Phe Gly Val Asn Leu 325 330 335Asp Asn Pro His Ala Gly Gly Gly Gly Gly Ala Ala Ala Gly Glu Ser 340 345 350Ser Asn His Gly Asn Ala Leu Ser Leu Gln Thr Pro Ala Trp Met Arg 355 360 365Arg Asp Pro Thr Leu Arg Leu Leu Glu Leu Pro Pro His His His His 370 375 380Gly Ala Glu Ser Ser Ala Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser385 390 395 400Lys Arg Asp Ala His Ser Ala Leu Asp Leu Asp Leu 405 410541239DNAOryza sativa 54atggagttca ctacaagcag taggttttct aaagaagagg aggacgagga gcaggatgag 60gcgggaaggc gagagatccc cttcatgacg gccacggccg aagccgcgcc tgcgcccacg 120tcgtcgtcgt cgtctcctgc tcatcacgcg gcttccgcgt cggcgtcggc gtctgcgtca 180gggagcagca ctccctttcg ctccgacgat ggcgccgggg cgtctgggag cggcggcggc 240ggcggcggcg gcggagaagc ggaggtggtg gagaaggagc acatgttcga caaggtggtg 300acgccgagcg acgttgggaa gctgaaccgg ctggtgatcc cgaagcagta cgccgagaag 360tacttcccgc tggacgcggc ggcgaacgag aagggcctcc tgctcaactt cgaggaccgc 420gcggggaagc catggcggtt ccgctactcc tactggaaca gcagccagag ctacgtgatg 480accaaggggt ggagccgctt cgtcaaggag aagcgcctcg acgccgggga caccgtctcc 540ttctcccgcg gcatcggcga cgaggcggcg cggcaccgcc tcttcatcga ctggaagcgc 600cgcgccgaca cccgcgaccc gctccggctg ccccgcgggc tgccgctccc gatgccgctc 660acgtcgcact acgccccgtg ggggatcggc ggcggagggg gattcttcgt gcagccctcg 720ccgccggcca cgctctacga gcaccgcctc aggcaaggcc tcgacttccg cgccttcaac 780cccgccgccg cgatggggag gcaggtcctc ctgttcggct cggcgaggat tcctccgcaa 840gcaccactgc tggcgcgcgc gccgtcgccg ctgcaccacc actacacgct gcagccgagc 900ggcgatggtg taagggcggc gggctcaccg gtggtgctcg actcggttcc ggtcatcgag 960agccccacga cggccgcgaa gcgcgtgcgg ctgttcggcg tgaacctcga caacccgcat 1020gccggcggcg gcggcggcgc cgccgccggc gagtcgagca atcatggcaa tgcactgtca 1080ttgcagacgc ccgcgtggat gaggagggat ccaacactgc ggctgctgga attgcctcct 1140caccaccacc atggcgccga gtcgtccgct gcatcgtctc cgtcgtcgtc gtcttcctcc 1200aagagggacg cgcattcggc cttggatctc gatctgtag 123955951DNAOryza sativa 55atggagtttg ctacaacgag tagtaggttt tccaaggaag aggaggagga ggaggaaggg 60gaacaggaga tggagcagga gcaggatgaa gaggaggagg aggcggaggc ctcgccccgc 120gagatcccct tcatgacgtc ggcggcggcg gcggccaccg cctcatcgtc ctccccgaca 180tcggtctccc cttccgccac cgcttccgcg gcggcgtcca cgtcggcgtc gggctctccc 240ttccggtcga gcgacggtgc gggagcgtcg gggagtggcg gcggcggtgg cggcgaggac 300gtggaggtga tcgagaagga gcacatgttc gacaaggtgg tgacgccgag cgacgtgggg 360aagctgaacc ggctggtgat cccgaagcag cacgccgaga agtacttccc gctggactcg 420gcggcgaacg agaagggcct tctcctcagc ttcgaggacc gaaccggcaa gctatggcgc 480ttccgctact cctactggaa cagcagccag agctacgtca tgaccaaggg ttggagccgc 540ttcgtcaagg agaagcgcct cgacgccggg gacaccgtct ccttctgccg cggcgccgcc 600gaggccaccc gcgaccgcct cttcatcgac tggaagcgcc gcgccgacgt ccgcgacccg 660caccgcttcc agcgcctacc gctccccatg acctcgccct acggcccgtg gggcggcggc 720gcgggcgctt cttcatgccg cccgcgccgc ccgccacgct ctacgagcat caccgctttc 780gccagggctt cgacttccgc aacatcaacc ccgctgtgcc ggcgaggcag ctcgtcttct 840tcggctcccc agggacgggg attcatcagc acccgccctt gccaccgccg ccgtcgccac 900ctccgcctcc tcaccaactc cacattacgg tgcaccaccc gagccccgta g 95156316PRTOryza sativa 56Met Glu Phe Ala Thr Thr Ser Ser Arg Phe Ser Lys Glu Glu Glu Glu1 5 10 15Glu Glu Glu Gly Glu Gln Glu Met Glu Gln Glu Gln Asp Glu Glu Glu 20 25 30Glu Glu Ala Glu Ala Ser Pro Arg Glu Ile Pro Phe Met Thr Ser Ala 35 40 45Ala Ala Ala Ala Thr Ala Ser Ser Ser Ser Pro Thr Ser Val Ser Pro 50 55 60Ser Ala Thr Ala Ser Ala Ala Ala Ser Thr Ser Ala Ser Gly Ser Pro65 70 75 80Phe Arg Ser Ser Asp Gly Ala Gly Ala Ser Gly Ser Gly Gly Gly Gly 85 90 95Gly Gly Glu Asp Val Glu Val Ile Glu Lys Glu His Met Phe Asp Lys 100 105 110Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 115 120 125Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ser Ala Ala Asn Glu 130 135 140Lys Gly Leu Leu Leu Ser Phe Glu Asp Arg Thr Gly Lys Leu Trp Arg145 150 155 160Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys 165 170 175Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr 180 185 190Val Ser Phe Cys Arg Gly Ala Ala Glu Ala Thr Arg Asp Arg Leu Phe 195 200 205Ile Asp Trp Lys Arg Arg Ala Asp Val Arg Asp Pro His Arg Phe Gln 210 215 220Arg Leu Pro Leu Pro Met Thr Ser Pro Tyr Gly Pro Trp Gly Gly Gly225 230 235 240Ala Gly Ala Ser Ser Cys Arg Pro Arg Arg Pro Pro Arg Ser Thr Ser 245 250 255Ile Thr Ala Phe Ala Arg Ala Ser Thr Ser Ala Thr Ser Thr Pro Leu 260 265 270Cys Arg Arg Gly Ser Ser Ser Ser Ser Ala Pro Gln Gly Arg Gly Phe 275 280 285Ile Ser Thr Arg Pro Cys His Arg Arg Arg Arg His Leu Arg Leu Leu 290 295 300Thr Asn Ser Thr Leu Arg Cys Thr Thr Arg Ala Pro305 310 31557936DNAOryza sativa 57atggagttca tcacgccaat cgtgaggccg gcatcggcgg cggcgggcgg cggcgaggtg 60caggagagtg gtgggaggag cttggcggcg gtggagaagg agcacatgtt cgacaaggtg 120gtgacgccga gcgacgtggg gaagctgaac cggctggtga tcccgaagca gcacgcggag 180aagtacttcc cgctggacgc ggcgtccaac gagaaggggc tcctgctcag cttcgaggac 240cgcacgggga agccatggcg gttccgctac tcctactgga acagcagcca gagctacgtg 300atgaccaagg ggtggagccg cttcgtcaag gagaagcgac tcgacgccgg ggacaccgtc 360tccttcggcc gcggcgtcgg cgaggccgcg cgcgggaggc tcttcatcga ctggcgccgc 420cgccccgacg tcgtcgccgc gctccagccg cccacgcacc gcttcgccca ccacctccct 480tcctccatcc ccttcgctcc ctgggcgcac caccacggac acggagccgc cgccgccgcc 540gccgccgccg ccggcgccag gtttctcctg cctccctcct cgactcccat ctacgaccac 600caccgccgac acgcccacgc cgtcgggtac gacgcgtacg ccgcggccac cagcaggcag 660gtgctgttct accggccgtt gccgccgcag cagcagcatc atcccgcggt ggtgctggag 720tcggtgccgg tgcgcatgac ggcggggcac gcggagccgc cgtcggctcc gtcgaagcga 780gttcggctgt tcggggtgaa cctcgactgc gcgaattccg aacaagacca cgccggcgtg 840gtcgggaaga cggcgccgcc gccgctgcca tcgccgccgt catcatcgtc atcttcctcc 900gggaaagcga ggtgctcctt gaaccttgac ttgtga 93658311PRTOryza sativa 58Met Glu Phe Ile Thr Pro Ile Val Arg Pro Ala Ser Ala Ala Ala Gly1 5 10 15Gly Gly Glu Val Gln Glu Ser Gly Gly Arg Ser Leu Ala Ala Val Glu 20 25 30Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys 35 40 45Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro 50 55 60Leu Asp Ala Ala Ser Asn Glu Lys Gly Leu Leu Leu Ser Phe Glu Asp65 70 75 80Arg Thr Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 85 90 95Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 100 105 110Arg Leu Asp Ala Gly Asp Thr Val Ser Phe Gly Arg Gly Val Gly Glu 115 120 125Ala Ala Arg Gly Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Asp Val 130 135 140Val Ala Ala Leu Gln Pro Pro Thr His Arg Phe Ala His His Leu Pro145 150 155 160Ser Ser Ile Pro Phe Ala Pro Trp Ala His His His Gly His Gly Ala 165 170 175Ala Ala Ala Ala Ala Ala Ala Ala Gly Ala Arg Phe Leu Leu Pro Pro 180 185 190Ser Ser Thr Pro Ile Tyr Asp His His Arg Arg His Ala His Ala Val 195 200 205Gly Tyr Asp Ala Tyr Ala Ala Ala Thr Ser Arg Gln Val Leu Phe Tyr 210 215 220Arg Pro Leu Pro Pro Gln Gln Gln His His Pro Ala Val Val Leu Glu225 230 235 240Ser Val Pro Val Arg Met Thr Ala Gly His Ala Glu Pro Pro Ser Ala 245 250 255Pro Ser Lys Arg Val Arg Leu Phe Gly Val Asn Leu Asp Cys Ala Asn 260 265 270Ser Glu Gln Asp His Ala Gly Val Val Gly Lys Thr Ala Pro Pro Pro 275 280 285Leu Pro Ser Pro Pro Ser Ser Ser Ser Ser Ser Ser Gly Lys Ala Arg 290 295 300Cys Ser Leu Asn Leu Asp Leu305 310591182DNAOryza sativa 59atggacagct ccagctgcct ggtggatgat accaacagcg gcggctcgtc cacggacaag 60ctgagggcgt tggccgccgc ggcggcggag acggcgccgc tggagcgcat ggggagcggg 120gcgagcgcgg tggtggacgc ggccgagcct ggcgcggagg cggactccgg gtccggggga 180cgtgtgtgcg gcggcggcgg cggcggtgcc ggcggtgcgg gagggaagct gccgtcgtcc 240aagttcaagg gcgtcgtgcc gcagcccaac gggaggtggg gcgcgcagat ctacgagcgg 300caccagcggg tgtggctcgg cacgttcgcc ggggaggacg acgccgcgcg cgcctacgac 360gtcgccgcgc agcgcttccg cggccgcgac gccgtcacca acttccgccc gctcgccgag 420gccgacccgg acgccgccgc cgagcttcgc ttcctcgcca cgcgctccaa ggccgaggtc 480gtcgacatgc tccgcaagca cacctacttc gacgagctcg cgcagagcaa gcgcaccttc 540gccgcctcca cgccgtcggc cgcgaccacc accgcctccc tctccaacgg ccacctctcg 600tcgccccgct cccccttcgc gcccgccgcg gcgcgcgacc acctgttcga caagacggtc 660accccgagcg acgtgggcaa gctgaacagg ctcgtcatac cgaagcagca cgccgagaag 720cacttcccgc tacagctccc gtccgccggc ggcgagagca agggtgtcct cctcaacttc 780gaggacgccg ccggcaaggt gtggcggttc cggtactcgt actggaacag cagccagagc 840tacgtgctaa ccaagggctg gagccgcttc gtcaaggaga agggtctcca cgccggcgac 900gtcgtcggct tctaccgctc cgccgccagt gccggcgacg acggcaagct cttcatcgac 960tgcaagttag tacggtcgac cggcgccgcc ctcgcgtcgc ccgctgatca gccagcgccg 1020tcgccggtga aggccgtcag gctcttcggc gtggacctgc tcacggcgcc ggcgccggtc 1080gaacagatgg ccgggtgcaa gagagccagg gacttggcgg cgacgacgcc tccacaagcg 1140gcggcgttca agaagcaatg catagagctg gcactagtat ag 118260393PRTOryza sativa 60Met Asp Ser Ser Ser Cys Leu Val Asp Asp Thr Asn Ser Gly Gly Ser1 5 10 15Ser Thr Asp Lys Leu Arg Ala Leu Ala Ala Ala Ala Ala Glu Thr Ala 20 25 30Pro Leu Glu Arg Met Gly Ser Gly Ala Ser Ala Val Val Asp Ala Ala 35 40 45Glu Pro Gly Ala Glu Ala Asp Ser Gly Ser Gly Gly Arg Val Cys Gly 50 55 60Gly Gly Gly Gly Gly Ala Gly Gly Ala Gly Gly Lys Leu Pro Ser Ser65 70 75 80Lys Phe Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln 85 90 95Ile Tyr Glu Arg His Gln Arg Val Trp Leu Gly Thr Phe Ala Gly Glu 100 105 110Asp Asp Ala Ala Arg Ala Tyr Asp Val Ala Ala Gln Arg Phe Arg Gly 115 120 125Arg Asp Ala Val Thr Asn Phe Arg Pro Leu Ala Glu Ala Asp Pro Asp 130 135 140Ala Ala Ala Glu Leu Arg Phe Leu Ala Thr Arg Ser Lys Ala Glu Val145 150 155 160Val Asp

Met Leu Arg Lys His Thr Tyr Phe Asp Glu Leu Ala Gln Ser 165 170 175Lys Arg Thr Phe Ala Ala Ser Thr Pro Ser Ala Ala Thr Thr Thr Ala 180 185 190Ser Leu Ser Asn Gly His Leu Ser Ser Pro Arg Ser Pro Phe Ala Pro 195 200 205Ala Ala Ala Arg Asp His Leu Phe Asp Lys Thr Val Thr Pro Ser Asp 210 215 220Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys225 230 235 240His Phe Pro Leu Gln Leu Pro Ser Ala Gly Gly Glu Ser Lys Gly Val 245 250 255Leu Leu Asn Phe Glu Asp Ala Ala Gly Lys Val Trp Arg Phe Arg Tyr 260 265 270Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser 275 280 285Arg Phe Val Lys Glu Lys Gly Leu His Ala Gly Asp Val Val Gly Phe 290 295 300Tyr Arg Ser Ala Ala Ser Ala Gly Asp Asp Gly Lys Leu Phe Ile Asp305 310 315 320Cys Lys Leu Val Arg Ser Thr Gly Ala Ala Leu Ala Ser Pro Ala Asp 325 330 335Gln Pro Ala Pro Ser Pro Val Lys Ala Val Arg Leu Phe Gly Val Asp 340 345 350Leu Leu Thr Ala Pro Ala Pro Val Glu Gln Met Ala Gly Cys Lys Arg 355 360 365Ala Arg Asp Leu Ala Ala Thr Thr Pro Pro Gln Ala Ala Ala Phe Lys 370 375 380Lys Gln Cys Ile Glu Leu Ala Leu Val385 39061939DNAOryza sativa 61atggagttca ccccaatttc gccgccgacg agggtcgccg gcggtgagga ggattccgag 60aggggggcgg cggcgtgggc ggtggtggag aaggagcaca tgtttgagaa ggtcgtgacg 120ccgagcgacg tggggaagct gaaccgattg gtcatcccca agcagcacgc cgagaggtac 180ttcccgctcg acgccgcggc gggcgccggc ggcggcggtg gtggcggcgg tggcggcggc 240ggggggaagg ggctggtgct gagcttcgag gacaggacgg ggaaggcgtg gaggttccgg 300tactcgtact ggaacagcag ccagagctac gtgatgacca aagggtggag ccgcttcgtc 360aaggagaagc gcctcggcgc cggcgacacc gtgtcgttcg gccgcggcct cggcgacgcc 420gcccgcggcc gcctcttcat cgacttccgc cgccgccgcc aggacgccgg cagcttcatg 480ttcccgccga cggcggcgcc gccgtcgcac tcgcaccacc atcatcagcg acaccacccg 540ccgctcccgt ccgtgcccct ttgcccgtgg cgagactaca ccaccgccta tggcggcggc 600tacggctacg gctacggcgg cggctccacc ccggcgtcca gccgccacgt gctgttcctc 660cggccgcagg tgccggccgc tgtggtgctc aagtcggtgc cggtgcacgt cgcggccacc 720tcggcggtgc aggaggcggc gacgacgaca aggccgaagc gtgtccggct gttcggggtg 780aacctcgact gcccggcggc catggacgac gacgacgaca tcgccggagc ggcgagccgg 840acggcagcgt cgtctctcct gcagctcccc tcgccgtcgt cctcgacgtc gtcgtcgacg 900gcggggaaga agatgtgctc cttggatctt gggttgtga 93962312PRTOryza sativa 62Met Glu Phe Thr Pro Ile Ser Pro Pro Thr Arg Val Ala Gly Gly Glu1 5 10 15Glu Asp Ser Glu Arg Gly Ala Ala Ala Trp Ala Val Val Glu Lys Glu 20 25 30His Met Phe Glu Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn 35 40 45Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Leu Asp 50 55 60Ala Ala Ala Gly Ala Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly65 70 75 80Gly Gly Lys Gly Leu Val Leu Ser Phe Glu Asp Arg Thr Gly Lys Ala 85 90 95Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met 100 105 110Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Gly Ala Gly 115 120 125Asp Thr Val Ser Phe Gly Arg Gly Leu Gly Asp Ala Ala Arg Gly Arg 130 135 140Leu Phe Ile Asp Phe Arg Arg Arg Arg Gln Asp Ala Gly Ser Phe Met145 150 155 160Phe Pro Pro Thr Ala Ala Pro Pro Ser His Ser His His His His Gln 165 170 175Arg His His Pro Pro Leu Pro Ser Val Pro Leu Cys Pro Trp Arg Asp 180 185 190Tyr Thr Thr Ala Tyr Gly Gly Gly Tyr Gly Tyr Gly Tyr Gly Gly Gly 195 200 205Ser Thr Pro Ala Ser Ser Arg His Val Leu Phe Leu Arg Pro Gln Val 210 215 220Pro Ala Ala Val Val Leu Lys Ser Val Pro Val His Val Ala Ala Thr225 230 235 240Ser Ala Val Gln Glu Ala Ala Thr Thr Thr Arg Pro Lys Arg Val Arg 245 250 255Leu Phe Gly Val Asn Leu Asp Cys Pro Ala Ala Met Asp Asp Asp Asp 260 265 270Asp Ile Ala Gly Ala Ala Ser Arg Thr Ala Ala Ser Ser Leu Leu Gln 275 280 285Leu Pro Ser Pro Ser Ser Ser Thr Ser Ser Ser Thr Ala Gly Lys Lys 290 295 300Met Cys Ser Leu Asp Leu Gly Leu305 31063337PRTGlycine max 63Met Ser Ile Asn His Tyr Ser Met Asp Leu Pro Glu Pro Thr Leu Trp1 5 10 15Trp Pro His Pro His His Gln Gln Gln Gln Leu Thr Leu Met Asp Pro 20 25 30Asp Pro Leu Arg Leu Asn Leu Asn Ser Asp Asp Gly Asn Gly Asn Asp 35 40 45Asn Asp Asn Asp Glu Asn Gln Thr Thr Thr Thr Gly Gly Glu Gln Glu 50 55 60Ile Leu Asp Asp Lys Glu Pro Met Phe Glu Lys Pro Leu Thr Pro Ser65 70 75 80Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu 85 90 95Lys Tyr Phe Pro Leu Ser Gly Asp Ser Gly Gly Ser Glu Cys Lys Gly 100 105 110Leu Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys Cys Trp Arg Phe Arg 115 120 125Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp 130 135 140Ser Arg Tyr Val Lys Asp Lys Arg Leu Asp Ala Gly Asp Val Val Leu145 150 155 160Phe Glu Arg His Arg Val Asp Ala Gln Arg Leu Phe Ile Gly Trp Arg 165 170 175Arg Arg Arg Gln Ser Asp Ala Ala Leu Pro Pro Ala His Val Ser Ser 180 185 190Arg Lys Ser Gly Gly Gly Asp Gly Asn Ser Asn Lys Asn Glu Gly Trp 195 200 205Thr Arg Gly Phe Tyr Ser Ala His His Pro Tyr Pro Thr His His Leu 210 215 220His His His Gln Pro Ser Pro Tyr Gln Gln Gln His Asp Cys Leu His225 230 235 240Ala Gly Arg Gly Ser Gln Gly Gln Asn Gln Arg Met Arg Pro Val Gly 245 250 255Asn Asn Ser Ser Ser Ser Ser Ser Ser Ser Arg Val Leu Arg Leu Phe 260 265 270Gly Val Asp Met Glu Cys Gln Pro Glu His Asp Asp Ser Gly Pro Ser 275 280 285Thr Pro Gln Cys Ser Tyr Asn Ser Asn Asn Met Leu Pro Ser Thr Gln 290 295 300Gly Thr Asp His Ser His His Asn Phe Tyr Gln Gln Gln Pro Ser Asn305 310 315 320Ser Asn Pro Ser Pro His His Met Met Val His His Gln Pro Tyr Tyr 325 330 335Tyr641014DNAGlycine max 64atgtccataa accactactc catggacctt cccgaaccga cactctggtg gccacaccca 60caccaccaac aacaacaact aaccttaatg gatcctgacc ctctccgtct caacctcaat 120agcgacgatg gcaatggcaa tgacaacgac aacgacgaaa atcaaacaac cacaacagga 180ggagaacaag aaatattaga cgataaagaa ccgatgttcg agaagccctt aaccccgagc 240gacgtgggga agctgaaccg tctcgtaatc ccgaagcagc acgcggagaa gtacttccca 300ctgagtggtg actcgggcgg gagcgagtgc aaggggctgt tactgagttt cgaggacgag 360tcggggaagt gttggcgctt ccgctactcg tactggaaca gcagccagag ctacgtgctc 420accaaagggt ggagccgcta cgtcaaggac aagcgccttg acgcgggcga cgtcgttttg 480ttcgagcgtc accgcgtcga cgcgcagcgc ctcttcatcg ggtggaggcg caggcggcag 540agcgatgccg ccttgccgcc tgcgcacgtt agcagtagga agagtggtgg tggtgatggg 600aatagtaata agaatgaggg gtggaccaga gggttctatt ctgcgcatca tccttatcct 660acgcatcatc ttcatcatca tcagccctcg ccataccaac aacaacatga ctgtcttcat 720gcaggtagag ggtcccaagg tcagaaccaa aggatgagac cagtgggaaa caacagttct 780agctctagtt cgagttcaag ggtacttagg ctgttcgggg tcgacatgga atgccaaccc 840gaacatgatg attctggtcc ctccacaccc caatgctcct acaatagtaa caacatgttg 900ccatcaacac agggcacaga tcattcccat cacaatttct accaacagca accttctaat 960tccaatcctt cccctcatca catgatggta catcaccaac catactacta ctag 101465344PRTGlycine max 65Met Ser Thr Asn His Tyr Thr Met Asp Leu Pro Glu Pro Thr Leu Trp1 5 10 15Trp Pro His Pro His Gln Gln Gln Leu Thr Leu Ile Asp Pro Asp Pro 20 25 30Leu Pro Leu Asn Leu Asn Asn Asp Asp Asn Asp Asn Gly Asp Asp Asn 35 40 45Asp Asn Asp Glu Asn Gln Thr Val Thr Thr Thr Thr Thr Gly Gly Glu 50 55 60Glu Glu Ile Ile Asn Asn Lys Glu Pro Met Phe Glu Lys Pro Leu Thr65 70 75 80Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His 85 90 95Ala Glu Lys Tyr Phe Pro Leu Ser Gly Gly Asp Ser Gly Ser Ser Glu 100 105 110Cys Lys Gly Leu Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys Cys Trp 115 120 125Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr 130 135 140Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys Arg Leu Asp Ala Gly Asp145 150 155 160Val Val Leu Phe Gln Arg His Arg Ala Asp Ala Gln Arg Leu Phe Ile 165 170 175Gly Trp Arg Arg Arg Arg Gln Ser Asp Ala Leu Pro Pro Pro Ala His 180 185 190Val Ser Ser Arg Lys Ser Gly Gly Asp Gly Asn Ser Ser Lys Asn Glu 195 200 205Gly Asp Val Gly Val Gly Trp Thr Arg Gly Phe Tyr Pro Ala His His 210 215 220Pro Tyr Pro Thr His His His His Pro Ser Pro Tyr His His Gln Gln225 230 235 240Asp Asp Ser Leu His Ala Val Arg Gly Ser Gln Gly Gln Asn Gln Arg 245 250 255Thr Arg Pro Val Gly Asn Ser Ser Ser Ser Ser Ser Ser Ser Ser Arg 260 265 270Val Leu Arg Leu Phe Gly Val Asn Met Glu Cys Gln Pro Glu His Asp 275 280 285Asp Ser Gly Pro Ser Thr Pro Gln Cys Ser Tyr Asn Thr Asn Asn Ile 290 295 300Leu Pro Ser Thr Gln Gly Thr Asp Ile His Ser His Leu Asn Phe Tyr305 310 315 320Gln Gln Gln Gln Thr Ser Asn Ser Lys Pro Pro Pro His His Met Met 325 330 335Ile Arg His Gln Pro Tyr Tyr Tyr 340661035DNAGlycine max 66atgtcgacaa accactacac catggacctt cccgaaccaa cactctggtg gccacaccca 60caccaacaac aactaacctt aatagatcca gaccctctcc ctctgaacct caacaacgac 120gacaacgaca atggcgacga caacgacaac gacgaaaacc aaacagttac aacaaccaca 180acaggaggag aagaagaaat aataaacaat aaagaaccga tgttcgagaa gccgctaacc 240ccgagcgacg tggggaagct gaaccgcctc gtaatcccga agcagcacgc tgagaagtac 300tttccactga gtggtggtga ctcgggcagt agcgagtgca aggggctgtt actgagtttc 360gaggacgagt cggggaagtg ctggcgcttc cgctactcgt actggaacag cagccagagc 420tacgtgctca ccaaagggtg gagccgttac gtgaaggaca agcgcctcga tgcgggagat 480gtcgttttat tccagcgcca ccgcgccgac gcgcagcgcc tcttcatcgg ctggaggcgc 540aggcggcaga gcgacgccct gccgccgcct gcgcacgtta gcagcaggaa gagtggtggt 600gatgggaata gtagtaagaa tgagggtgat gtgggcgtgg gctggaccag agggttctat 660cctgcgcatc atccttatcc tacgcatcat catcatccct cgccatacca tcaccaacaa 720gatgactctc ttcatgcagt tagagggtcc caaggtcaga accaaaggac gagaccagtg 780ggaaacagca gttctagttc gagttcgagt tcaagggtac ttaggctatt cggggtcaac 840atggaatgcc aacccgaaca tgatgattct ggaccctcca caccccaatg ctcctacaat 900actaacaaca tattgccatc cacacagggc acagatattc attcccatct caatttctac 960caacaacaac aaacttctaa ttccaagcct ccccctcatc acatgatgat acgtcaccaa 1020ccatactact actag 103567288PRTGlycine max 67Met Ser Ser Ile Asn His Tyr Ser Pro Glu Thr Thr Leu Tyr Trp Thr1 5 10 15Asn Asp Gln Gln Gln Gln Ala Ala Met Trp Leu Ser Asn Ser His Thr 20 25 30Pro Arg Phe Asn Leu Asn Asp Glu Glu Glu Glu Glu Glu Asp Asp Val 35 40 45Ile Val Ser Asp Lys Ala Thr Asn Asn Leu Thr Gln Glu Glu Glu Lys 50 55 60Val Ala Met Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys Leu65 70 75 80Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu 85 90 95Asp Ser Ser Ala Ala Lys Gly Leu Leu Leu Ser Phe Glu Asp Glu Ser 100 105 110Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser 115 120 125Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys Arg Leu 130 135 140His Ala Gly Asp Val Val Leu Phe His Arg His Arg Ser Leu Pro Gln145 150 155 160Arg Phe Phe Ile Ser Cys Ser Arg Arg Gln Pro Asn Pro Val Pro Ala 165 170 175His Val Ser Thr Thr Arg Ser Ser Ala Ser Phe Tyr Ser Ala His Pro 180 185 190Pro Tyr Pro Ala His His Phe Pro Phe Pro Tyr Gln Pro His Ser Leu 195 200 205His Ala Pro Gly Gly Gly Ser Gln Gly Gln Asn Glu Thr Thr Pro Gly 210 215 220Gly Asn Ser Ser Ser Ser Gly Ser Gly Arg Val Leu Arg Leu Phe Gly225 230 235 240Val Asn Met Glu Cys Gln Pro Asp Asn His Asn Asp Ser Gln Asn Ser 245 250 255Thr Pro Glu Cys Ser Tyr Thr His Leu Tyr His His Gln Thr Ser Ser 260 265 270Tyr Ser Ser Ser Ser Asn Pro His His His Met Val Pro Gln Gln Pro 275 280 28568867DNAGlycine max 68atgtcatcga taaaccacta ttcaccggaa acaacactat actggaccaa cgaccaacag 60caacaagccg ccatgtggct gagtaattcc cacaccccgc gtttcaatct gaacgacgag 120gaggaggagg aggaagacga cgttatcgtt tcggacaagg ctactaataa cttgacgcaa 180gaggaggaga aggtagccat gttcgagaag ccgttgacgc cgagcgacgt cgggaagctg 240aaccggctcg tgattccgaa acagcacgcg gagaagcact tccctctcga ctcgtcggcg 300gcgaaggggc tgttgctgag tttcgaggac gagtccggga agtgttggcg cttccgttac 360tcttattgga acagtagcca gagttacgtt ttgaccaaag gatggagccg ttacgtcaaa 420gacaaacgcc tccacgctgg cgacgtcgtt ttgttccaca gacaccgctc cctccctcaa 480cgcttcttca tctcctgcag ccgccgccaa cccaacccgg tccccgctca cgttagcacc 540accagatcct ccgcttcctt ctactctgcg cacccacctt atcctgcgca ccacttcccc 600ttcccatacc aacctcactc tcttcatgca ccaggtggag ggtcccaagg acagaacgaa 660acgacaccgg gagggaacag tagttcaagt ggcagtggca gggtgctgag gctctttggt 720gtgaacatgg aatgccaacc tgataatcat aatgattccc agaactccac accagaatgc 780tcctacaccc acttatacca ccatcaaacc tcttcttatt cttcttcttc aaaccctcac 840catcacatgg tacctcaaca accataa 86769420PRTGlycine max 69Met Glu Leu Met Gln Gln Val Lys Gly Asn Tyr Ser Asp Ser Arg Glu1 5 10 15Glu Glu Glu Glu Glu Glu Ala Ala Ala Ile Thr Arg Glu Ser Glu Ser 20 25 30Ser Arg Leu His Gln Gln Asp Thr Ala Ser Asn Phe Gly Lys Lys Leu 35 40 45Asp Leu Met Asp Leu Ser Leu Gly Ser Ser Lys Glu Glu Glu Glu Glu 50 55 60Gly Asn Leu Gln Gln Gly Gly Gly Gly Val Val His His Ala His Gln65 70 75 80Val Val Glu Lys Glu His Met Phe Glu Lys Val Ala Thr Pro Ser Asp 85 90 95Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys 100 105 110Tyr Phe Pro Leu Asp Ser Ser Thr Asn Glu Lys Gly Leu Leu Leu Asn 115 120 125Phe Glu Asp Arg Asn Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp 130 135 140Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val145 150 155 160Lys Glu Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Gly 165 170 175Leu Gly Asp Leu Tyr Arg His Arg Leu Tyr Ile Asp Trp Lys Arg Arg 180 185 190Pro Asp His Ala His Ala His Pro Pro His His His Asp Pro Leu Phe 195 200 205Leu Pro Ser Ile Arg Leu Tyr Ser Leu Pro Pro Thr Met Pro Pro Arg 210 215 220Tyr His His Asp His His Phe His His His Leu Asn Tyr Asn Asn Leu225 230 235 240Phe Thr Phe Gln Gln His Gln Tyr Gln Gln Leu Gly Ala Ala Thr Thr 245 250 255Thr His His Asn Asn Tyr Gly Tyr Gln Asn Ser Gly Ser Gly Ser Leu 260 265 270Tyr Tyr Leu Arg Ser Ser Met Ser Met Gly Gly Gly Asp Gln Asn Leu 275 280 285Gln Gly

Arg Gly Ser Asn Ile Val Pro Met Ile Ile Asp Ser Val Pro 290 295 300Val Asn Val Ala His His Asn Asn Asn Arg His Gly Asn Gly Gly Ile305 310 315 320Thr Ser Gly Gly Thr Asn Cys Ser Gly Lys Arg Leu Arg Leu Phe Gly 325 330 335Val Asn Met Glu Cys Ala Ser Ser Ala Glu Asp Ser Lys Glu Leu Ser 340 345 350Ser Gly Ser Ala Ala His Val Thr Thr Ala Ala Ser Ser Ser Ser Leu 355 360 365His His Gln Arg Leu Arg Val Pro Val Pro Val Pro Leu Glu Asp Pro 370 375 380Leu Ser Ser Ser Ala Ala Ala Ala Ala Arg Phe Gly Asp His Lys Gly385 390 395 400Ala Ser Thr Gly Thr Ser Leu Leu Phe Asp Leu Asp Pro Ser Leu Gln 405 410 415Tyr His Arg His 420701263DNAGlycine max 70atggagttga tgcaacaagt taaaggtaat tattctgata gcagggagga agaggaggaa 60gaggaagctg cagcaatcac aagggaatca gaaagcagca ggttacacca acaagataca 120gcatccaatt ttggaaagaa gctagacttg atggacttgt cactagggag cagcaaggaa 180gaggaagagg aagggaattt gcaacaagga ggaggaggag tggttcatca tgctcaccaa 240gtagtggaga aagaacacat gtttgagaaa gtggcgacac cgagcgacgt agggaagctg 300aacaggctgg tgataccgaa gcagcacgcg gagaagtact tcccccttga ctcctcaacc 360aacgagaagg gtctgctcct gaatttcgag gacaggaatg ggaaggtgtg gcgattcagg 420tattcctatt ggaacagcag ccagagctat gtgatgacaa aagggtggag ccgctttgtt 480aaggagaaga agctggatgc cggtgacatt gtctccttcc agcgtggcct tggggatttg 540tatagacatc ggttgtatat agattggaag agaaggcccg atcatgctca tgctcatcca 600cctcatcatc acgatccttt gtttcttccc tctatcagat tgtactctct ccctcccacc 660atgccacctc gctaccacca cgatcatcac tttcaccacc atctcaatta caacaacctc 720ttcacttttc agcaacacca gtaccagcag cttggtgctg ccactaccac tcatcacaac 780aactatggtt accagaattc gggatctggt tcactctatt acctaaggtc ctctatgtca 840atgggtggtg gtgatcaaaa cttgcaaggg agagggagca acattgtccc catgatcatt 900gattctgtgc cggttaacgt tgctcatcac aacaacaatc gccatgggaa tgggggcatc 960acgagtggtg gtactaattg tagtggaaaa cgactaaggc tatttggggt gaacatggaa 1020tgcgcttctt cggcagaaga ttccaaagaa ttgtcctcgg gttcggcagc acacgtgacg 1080acagctgctt cttcttcttc tcttcatcat cagcgcttga gggtgccagt gccagtgcca 1140cttgaagatc cactttcgtc gtcagcagca gcagcagcaa ggtttgggga tcacaaaggg 1200gccagtactg ggacttcgct gctgtttgat ttggatccct ctttgcagta tcatcgccac 1260tga 126371384PRTGlycine max 71Met Asp Ala Ile Ser Cys Leu Asp Glu Ser Thr Thr Thr Glu Ser Leu1 5 10 15Ser Ile Ser Gln Ala Lys Pro Ser Ser Thr Ile Met Ser Ser Glu Lys 20 25 30Ala Ser Pro Ser Pro Pro Pro Pro Asn Arg Leu Cys Arg Val Gly Ser 35 40 45Gly Ala Ser Ala Val Val Asp Ser Asp Gly Gly Gly Gly Gly Gly Ser 50 55 60Thr Glu Val Glu Ser Arg Lys Leu Pro Ser Ser Lys Tyr Lys Gly Val65 70 75 80Val Pro Gln Pro Asn Gly Arg Trp Gly Ser Gln Ile Tyr Glu Lys His 85 90 95Gln Arg Val Trp Leu Gly Thr Phe Asn Glu Glu Asp Glu Ala Ala Arg 100 105 110Ala Tyr Asp Val Ala Val Gln Arg Phe Arg Gly Lys Asp Ala Val Thr 115 120 125Asn Phe Lys Pro Leu Ser Gly Thr Asp Asp Asp Asp Gly Glu Ser Glu 130 135 140Phe Leu Asn Ser His Ser Lys Ser Glu Ile Val Asp Met Leu Arg Lys145 150 155 160His Thr Tyr Asn Asp Glu Leu Glu Gln Ser Lys Arg Ser Arg Gly Phe 165 170 175Val Arg Arg Arg Gly Ser Ala Ala Gly Ala Gly Asn Gly Asn Ser Ile 180 185 190Ser Gly Ala Cys Val Met Lys Ala Arg Glu Gln Leu Phe Gln Lys Ala 195 200 205Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys 210 215 220Gln His Ala Glu Lys His Phe Pro Leu Gln Ser Ala Ala Asn Gly Val225 230 235 240Ser Ala Thr Ala Thr Ala Ala Lys Gly Val Leu Leu Asn Phe Glu Asp 245 250 255Val Gly Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 260 265 270Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 275 280 285Asn Leu Lys Ala Gly Asp Thr Val Cys Phe Gln Arg Ser Thr Gly Pro 290 295 300Asp Arg Gln Leu Tyr Ile Asp Trp Lys Thr Arg Asn Val Val Asn Glu305 310 315 320Val Ala Leu Phe Gly Pro Val Val Glu Pro Ile Gln Met Val Arg Leu 325 330 335Phe Gly Val Asn Ile Leu Lys Leu Pro Gly Ser Asp Ser Ile Ala Asn 340 345 350Asn Asn Asn Ala Ser Gly Cys Cys Asn Gly Lys Arg Arg Glu Met Glu 355 360 365Leu Phe Ser Leu Glu Cys Ser Lys Lys Pro Lys Ile Ile Gly Ala Leu 370 375 380721155DNAGlycine max 72atggatgcaa ttagttgcct ggatgagagc accaccaccg agtcactctc cataagtcag 60gcgaagcctt cttcgacgat tatgtcgtcc gagaaggctt ctccttcccc gccgccgccg 120aacaggctgt gccgcgtcgg tagcggtgct agcgcagtcg tggattccga cggcggcggc 180gggggtggca gcaccgaggt ggagtcgcgg aagctcccct cgtccaagta taagggcgtc 240gtgccccagc ccaacggccg ctggggctcg cagatttacg agaagcacca gcgcgtgtgg 300ctgggaacgt tcaacgagga agacgaggcg gcgcgtgcgt acgacgtcgc cgtgcagcga 360ttccgcggca aggacgccgt cacaaacttc aagccgctct ccggcaccga cgacgacgac 420ggggaatcgg agtttctcaa ctcgcattcg aaatccgaga tcgtcgacat gctgcgtaag 480catacgtaca atgacgagct ggaacaaagc aagcgcagcc gcggcttcgt acgtcggcgc 540ggctccgccg ccggcgccgg aaacggaaac tcaatctccg gcgcgtgtgt tatgaaggcg 600cgtgagcagc tattccagaa ggccgttacg ccgagcgacg ttgggaaact gaaccgtttg 660gtgataccga agcagcacgc ggagaagcac tttcctttac agagcgctgc taacggcgtt 720agcgcgacgg cgacggcggc gaagggcgtt ttgttgaact tcgaagacgt tggagggaaa 780gtgtggcggt ttcgttactc gtattggaac agtagccaga gttacgtctt gaccaaaggt 840tggagccggt tcgttaagga gaagaatctg aaagccggtg acacggtttg ttttcaacgg 900tccactggac cggacaggca gctttacatc gattggaaga cgaggaatgt tgttaacgag 960gtcgcgttgt tcggaccggt tgtcgaaccg atccagatgg ttcggctctt tggtgttaac 1020attttgaaac tacccggttc agattctatc gccaataaca ataatgcaag tgggtgctgc 1080aatggcaaga gaagagaaat ggaactcttt tcattagagt gtagcaagaa acctaagatt 1140attggtgctt tgtag 115573491PRTGlycine max 73Met Glu Leu Met Gln Glu Val Lys Gly Tyr Ser Asp Gly Arg Glu Glu1 5 10 15Glu Glu Glu Glu Glu Glu Ala Ala Glu Glu Ile Ile Thr Arg Glu Glu 20 25 30Ser Ser Arg Leu Leu His Gln His Gln Glu Ala Ala Gly Ser Asn Phe 35 40 45Ile Ile Asn Asn Asn His His His His Gln His His His His His Thr 50 55 60Thr Lys Gln Leu Asp Phe Met Asp Leu Ser Leu Gly Ser Ser Lys Asp65 70 75 80Glu Gly Asn Leu Gln Gly Ser Ser Ser Ser Val Tyr Ala His His His 85 90 95His Ala Ala Ser Ala Ser Ser Ser Ala Asn Gly Asn Asn Asn Asn Ser 100 105 110Ser Ser Ser Asn Leu Gln Gln Gln Gln Gln Gln Pro Ala Glu Lys Glu 115 120 125His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn 130 135 140Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp145 150 155 160Ser Ser Ala Asn Glu Lys Gly Leu Leu Leu Asn Phe Glu Asp Arg Asn 165 170 175Gly Lys Leu Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser 180 185 190Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Lys Leu 195 200 205Asp Ala Gly Asp Met Val Ser Phe Gln Arg Gly Val Gly Glu Leu Tyr 210 215 220Arg His Arg Leu Tyr Ile Asp Trp Trp Arg Arg Pro Asp His His His225 230 235 240His His His His Gly Pro Asp His Ser Thr Thr Leu Phe Thr Pro Phe 245 250 255Leu Ile Pro Asn Gln Pro His His Leu Met Ser Ile Arg Trp Gly Ala 260 265 270Thr Gly Arg Leu Tyr Ser Leu Pro Ser Pro Thr Pro Pro Arg His His 275 280 285Glu His Leu Asn Tyr Asn Asn Asn Ala Met Tyr His Pro Phe His His 290 295 300His Gly Ala Gly Ser Gly Ile Asn Ala Thr Thr His His Tyr Asn Asn305 310 315 320Tyr His Glu Met Ser Ser Thr Thr Thr Ser Gly Ser Ala Gly Ser Val 325 330 335Phe Tyr His Arg Ser Thr Pro Pro Ile Ser Met Pro Leu Ala Asp His 340 345 350Gln Thr Leu Asn Thr Arg Gln Gln Gln Gln Gln Gln Gln Gln Gln Glu 355 360 365Gly Ala Gly Asn Val Ser Leu Ser Pro Met Ile Ile Asp Ser Val Pro 370 375 380Val Ala His His Leu His His Gln Gln His His Gly Gly Lys Ser Ser385 390 395 400Gly Pro Ser Ser Thr Ser Thr Ser Pro Ser Thr Ala Gly Lys Arg Leu 405 410 415Arg Leu Phe Gly Val Asn Met Glu Cys Ala Ser Ser Thr Ser Glu Asp 420 425 430Pro Lys Cys Phe Ser Leu Leu Ser Ser Ser Ser Met Ala Asn Ser Asn 435 440 445Ser Gln Pro Pro Leu Gln Leu Leu Arg Glu Asp Thr Leu Ser Ser Ser 450 455 460Ser Ala Arg Phe Gly Asp Gln Arg Gly Val Gly Glu Pro Ser Met Leu465 470 475 480Phe Asp Leu Asp Pro Ser Leu Gln Tyr Arg Gln 485 490741476DNAGlycine max 74atggagttga tgcaagaagt gaaagggtat tctgatggca gagaggagga ggaggaggaa 60gaggaagcag cagaagaaat catcacaaga gaagaaagca gcaggttgtt acaccagcac 120caggaggcag caggttccaa tttcatcatc aacaataatc atcatcatca tcaacatcac 180caccaccaca caacaaagca gctagacttc atggacttgt cacttggtag cagcaaggat 240gaagggaatt tgcaaggatc atcttcttct gtctatgctc atcatcatca tgcagcaagt 300gctagttctt ctgccaatgg taacaacaac aacagcagca gcagcaactt gcagcaacag 360cagcagcagc ctgctgagaa ggagcacatg tttgataaag tagtgacacc aagtgatgtg 420gggaagctga accggttggt gataccaaag cagcatgctg agaagtattt ccctcttgat 480tcctcagcca atgagaaggg tctgttgctg aattttgagg acaggaatgg taagttgtgg 540aggttcaggt actcctattg gaacagcagc cagagctatg tgatgaccaa aggttggagc 600cgttttgtta aggagaagaa gcttgatgct ggtgacatgg tgtccttcca gcgtggtgtt 660ggggagttgt ataggcatag gttgtacata gattggtgga gaaggcctga tcatcatcac 720catcaccatc atggccctga ccattcaacc acactcttca cacctttctt aattcccaat 780cagcctcatc acttaatgtc catcagatgg ggtgccactg gcagattgta ctccctccct 840tccccaaccc caccacgcca ccatgaacac ctcaattaca acaataacgc catgtatcat 900ccctttcatc accatggtgc tggaagtgga attaatgcta ctactcatca ctacaacaac 960tatcatgaga tgagtagtac tactacttca ggatctgcag gctcagtctt ttaccacagg 1020tcaacacccc caatatcaat gccattggct gaccaccaaa ccttgaacac aaggcagcag 1080caacaacaac aacaacaaca agagggagct ggcaatgttt ctctttcccc tatgatcatt 1140gattctgttc cagttgctca ccacctccat catcaacaac accatggtgg caagagtagt 1200ggtcctagta gtactagtac tagtcctagc actgcaggga aaagactaag gctatttggg 1260gtcaacatgg aatgtgcttc ttcaacatca gaagacccca aatgcttcag cttgttgtcc 1320tcatcttcaa tggctaattc caattcacaa ccaccacttc agcttttgag ggaagataca 1380ctttcgtcat catcggcaag gtttggggat cagagaggag taggggaacc ttcaatgctt 1440tttgatctgg acccttcttt gcaataccgg cagtga 147675351PRTGlycine max 75Met Asp Gly Gly Cys Val Thr Asp Glu Thr Thr Thr Ser Ser Asp Ser1 5 10 15Leu Ser Val Pro Pro Pro Ser Arg Val Gly Ser Val Ala Ser Ala Val 20 25 30Val Asp Pro Asp Gly Cys Cys Val Ser Gly Glu Ala Glu Ser Arg Lys 35 40 45Leu Pro Ser Ser Lys Tyr Lys Gly Val Val Pro Gln Pro Asn Gly Arg 50 55 60Trp Gly Ala Gln Ile Tyr Glu Lys His Gln Arg Val Trp Leu Gly Thr65 70 75 80Phe Asn Glu Glu Asp Glu Ala Ala Arg Ala Tyr Asp Ile Ala Ala Leu 85 90 95Arg Phe Arg Gly Pro Asp Ala Val Thr Asn Phe Lys Pro Pro Ala Ala 100 105 110Ser Asp Asp Ala Glu Ser Glu Phe Leu Asn Ser His Ser Lys Phe Glu 115 120 125Ile Val Asp Met Leu Arg Lys His Thr Tyr Asp Asp Glu Leu Gln Gln 130 135 140Ser Thr Arg Gly Gly Arg Arg Arg Leu Asp Ala Asp Thr Ala Ser Ser145 150 155 160Gly Val Phe Asp Ala Lys Ala Arg Glu Gln Leu Phe Glu Lys Thr Val 165 170 175Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln 180 185 190His Ala Glu Lys His Phe Pro Leu Ser Gly Ser Gly Asp Glu Ser Ser 195 200 205Pro Cys Val Ala Gly Ala Ser Ala Ala Lys Gly Met Leu Leu Asn Phe 210 215 220Glu Asp Val Gly Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn225 230 235 240Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys 245 250 255Glu Lys Asn Leu Arg Ala Gly Asp Ala Val Gln Phe Phe Lys Ser Thr 260 265 270Gly Pro Asp Arg Gln Leu Tyr Ile Asp Cys Lys Ala Arg Ser Gly Glu 275 280 285Val Asn Asn Asn Ala Gly Gly Leu Phe Val Pro Ile Gly Pro Val Val 290 295 300Glu Pro Val Gln Met Val Arg Leu Phe Gly Val Asn Leu Leu Lys Leu305 310 315 320Pro Val Pro Gly Ser Asp Gly Val Gly Lys Arg Lys Glu Met Glu Leu 325 330 335Phe Ala Phe Glu Cys Cys Lys Lys Leu Lys Val Ile Gly Ala Leu 340 345 350761056DNAGlycine max 76atggatggag gctgtgtcac agacgaaacc accacatcca gcgactctct ttccgttccg 60ccgcccagcc gcgtcggcag cgttgcaagc gccgtcgtcg accccgacgg ttgttgcgtt 120tccggcgagg ccgaatcccg gaaactccct tcgtcgaaat acaaaggcgt ggtgccgcaa 180ccgaacggtc gctggggagc tcagatttac gagaagcacc agcgcgtgtg gctcggcact 240ttcaacgagg aagacgaagc cgccagagcc tacgacatcg ccgcgctgcg cttccgcggc 300cccgacgccg tcaccaactt caagcctccc gccgcctccg acgacgccga gtccgagttc 360ctcaactcgc attccaagtt cgagatcgtc gacatgctcc gcaagcacac ctacgacgac 420gagctccagc agagcacgcg cggtggtagg cgccgcctcg acgctgacac cgcgtcgagc 480ggtgtgttcg acgcgaaagc gcgtgagcag ctgttcgaga aaacggttac gccgagcgac 540gtcgggaagc tgaatcgatt agtgataccg aagcagcacg cggagaagca ctttccgtta 600agcggatccg gcgacgaaag ctcgccgtgc gtggcggggg cttcggcggc gaagggaatg 660ttgttgaact ttgaggacgt tggagggaaa gtgtggcggt ttcgttactc ttattggaac 720agtagccaga gctacgtgct taccaaagga tggagccggt tcgttaagga gaagaatctt 780cgagccggtg acgcggttca gttcttcaag tcgaccggac cggaccggca gctatatata 840gactgcaagg cgaggagtgg tgaggttaac aataatgctg gcggtttgtt tgttccgatt 900ggaccggtcg ttgagccggt tcagatggtt cggcttttcg gggtcaacct tttgaaacta 960cccgtacccg gttcggatgg tgtagggaag agaaaagaga tggaactgtt tgcatttgaa 1020tgttgcaaga agttaaaagt aattggagct ttgtaa 105677401PRTGlycine max 77Met Asp Ala Ile Ser Cys Met Asp Glu Ser Thr Thr Thr Glu Ser Leu1 5 10 15Ser Ile Ser Leu Ser Pro Thr Ser Ser Ser Glu Lys Ala Lys Pro Ser 20 25 30Ser Met Ile Thr Ser Ser Glu Lys Val Ser Leu Ser Pro Pro Pro Ser 35 40 45Asn Arg Leu Cys Arg Val Gly Ser Gly Ala Ser Ala Val Val Asp Pro 50 55 60Asp Gly Gly Gly Ser Gly Ala Glu Val Glu Ser Arg Lys Leu Pro Ser65 70 75 80Ser Lys Tyr Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala 85 90 95Gln Ile Tyr Glu Lys His Gln Arg Val Trp Leu Gly Thr Phe Asn Glu 100 105 110Glu Asp Glu Ala Ala Arg Ala Tyr Asp Ile Ala Ala Gln Arg Phe Arg 115 120 125Gly Lys Asp Ala Val Thr Asn Phe Lys Pro Leu Ala Gly Ala Asp Asp 130 135 140Asp Asp Gly Glu Ser Glu Phe Leu Asn Ser His Ser Lys Pro Glu Ile145 150 155 160Val Asp Met Leu Arg Lys His Thr Tyr Asn Asp Glu Leu Glu Gln Ser 165 170 175Lys Arg Ser Arg Gly Val Val Arg Arg Arg Gly Ser Ala Ala Ala Gly 180 185 190Thr Ala Asn Ser Ile Ser Gly Ala Cys Phe Thr Lys Ala Arg Glu Gln 195 200 205Leu Phe Glu Lys Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg 210 215 220Leu Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Gln Ser225 230 235 240Ser Asn Gly Val Ser Ala Thr Thr Ile Ala Ala Val Thr Ala Thr Pro 245 250 255Thr Ala Ala

Lys Gly Val Leu Leu Asn Phe Glu Asp Val Gly Gly Lys 260 265 270Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 275 280 285Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Lys Ala 290 295 300Gly Asp Thr Val Cys Phe His Arg Ser Thr Gly Pro Asp Lys Gln Leu305 310 315 320Tyr Ile Asp Trp Lys Thr Arg Asn Val Val Asn Asn Glu Val Ala Leu 325 330 335Phe Gly Pro Val Gly Pro Val Val Glu Pro Ile Gln Met Val Arg Leu 340 345 350Phe Gly Val Asn Ile Leu Lys Leu Pro Gly Ser Asp Thr Ile Val Gly 355 360 365Asn Asn Asn Asn Ala Ser Gly Cys Cys Asn Gly Lys Arg Arg Glu Met 370 375 380Glu Leu Phe Ser Leu Glu Cys Ser Lys Lys Pro Lys Ile Ile Gly Ala385 390 395 400Leu781206DNAGlycine max 78atggatgcaa ttagttgcat ggatgagagc accaccactg agtcactctc tataagtctt 60tctccgacgt catcgtcgga gaaagcgaag ccttcttcga tgattacatc gtcggagaag 120gtttctctgt ccccgccgcc gtcaaacaga ctatgccgtg ttggaagcgg cgcgagcgca 180gtcgtggatc ctgatggcgg cggcagcggc gctgaggtag agtcgcggaa actcccctcg 240tcgaagtaca agggcgtggt gccccagccc aacggccgct ggggtgcgca gatttacgag 300aagcaccagc gcgtgtggct tggaacgttc aacgaggaag acgaggcggc gcgtgcgtac 360gacatcgccg cgcagcggtt ccgcggcaag gacgccgtca cgaacttcaa gccgctcgcc 420ggcgccgacg acgacgacgg agaatcggag tttctcaact cgcattccaa acccgagatc 480gtcgacatgc tgcgaaagca cacgtacaat gacgagctgg agcagagcaa gcgcagccgc 540ggcgtcgtcc ggcggcgagg ctccgccgcc gccggcaccg caaactcaat ttccggcgcg 600tgctttacta aggcacgtga gcagctattc gagaaggctg ttacgccgag cgacgttggg 660aaattgaacc gtttggtgat accgaagcag cacgcggaga agcactttcc gttacagagc 720tctaacggcg ttagcgcgac gacgatagcg gcggtgacgg cgacgccgac ggcggcgaag 780ggcgttttgt tgaacttcga agacgttgga gggaaagtgt ggcggtttcg ttactcgtat 840tggaacagta gccagagtta cgtcttaacc aaaggttgga gccggttcgt taaggagaag 900aatctgaaag ctggtgacac ggtttgtttt caccggtcca ctggaccgga caagcagctt 960tacatcgatt ggaagacgag gaatgttgtt aacaacgagg tcgcgttgtt cggaccggtc 1020ggaccggttg tcgaaccgat ccagatggtt cggctctttg gggttaacat tttgaaacta 1080cccggttcag atactattgt tggcaataac aataatgcaa gtgggtgctg caatggcaag 1140agaagagaaa tggaactgtt ctcgttagag tgtagcaaga aacctaagat tattggtgct 1200ttgtaa 120679362PRTGlycine max 79Met Asp Gly Gly Ser Val Thr Asp Glu Thr Thr Thr Thr Ser Asn Ser1 5 10 15Leu Ser Val Pro Ala Asn Leu Ser Pro Pro Pro Leu Ser Leu Val Gly 20 25 30Ser Gly Ala Thr Ala Val Val Tyr Pro Asp Gly Cys Cys Val Ser Gly 35 40 45Glu Ala Glu Ser Arg Lys Leu Pro Ser Ser Lys Tyr Lys Gly Val Val 50 55 60Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Lys His Gln65 70 75 80Arg Val Trp Leu Gly Thr Phe Asn Glu Glu Asp Glu Ala Ala Arg Ala 85 90 95Tyr Asp Ile Ala Ala His Arg Phe Arg Gly Arg Asp Ala Val Thr Asn 100 105 110Phe Lys Pro Leu Ala Gly Ala Asp Asp Ala Glu Ala Glu Phe Leu Ser 115 120 125Thr His Ser Lys Ser Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Asp Asn Glu Leu Gln Gln Ser Thr Arg Gly Gly Arg Arg Arg Arg Asp145 150 155 160Ala Glu Thr Ala Ser Ser Gly Ala Phe Asp Ala Lys Ala Arg Glu Gln 165 170 175Leu Phe Glu Lys Thr Val Thr Gln Ser Asp Val Gly Lys Leu Asn Arg 180 185 190Leu Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Ser Gly 195 200 205Ser Gly Gly Gly Ala Leu Pro Cys Met Ala Ala Ala Ala Gly Ala Lys 210 215 220Gly Met Leu Leu Asn Phe Glu Asp Val Gly Gly Lys Val Trp Arg Phe225 230 235 240Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly 245 250 255Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Arg Ala Gly Asp Ala Val 260 265 270Gln Phe Phe Lys Ser Thr Gly Leu Asp Arg Gln Leu Tyr Ile Asp Cys 275 280 285Lys Ala Arg Ser Gly Lys Val Asn Asn Asn Ala Ala Gly Leu Phe Ile 290 295 300Pro Val Gly Pro Val Val Glu Pro Val Gln Met Val Arg Leu Phe Gly305 310 315 320Val Asp Leu Leu Lys Leu Pro Val Pro Gly Ser Asp Gly Ile Gly Val 325 330 335Gly Cys Asp Gly Lys Arg Lys Glu Met Glu Leu Phe Ala Phe Glu Cys 340 345 350Ser Lys Lys Leu Lys Val Ile Gly Ala Leu 355 360801089DNAGlycine max 80atggatggag gcagtgtcac agacgaaacc accacaacca gcaactctct ttcggttccg 60gcgaatctat ctccgccgcc tctcagcctt gtcggcagcg gcgcaaccgc cgtcgtctac 120cccgacggtt gttgcgtctc cggcgaagcc gaatcccgga aactcccgtc ctcgaaatac 180aaaggcgtgg tgccgcaacc gaacggtcgt tggggagctc agatttacga gaagcaccag 240cgcgtgtggc tcggcacctt caacgaggaa gacgaagccg ccagagccta cgacatcgcc 300gcgcatcgct tccgcggccg cgacgccgtc actaacttca agcctctcgc cggcgccgac 360gacgccgaag ccgagttcct cagcacgcat tccaagtccg agatcgtcga catgctccgc 420aagcacacct acgacaacga gctccagcag agcacccgcg gcggcaggcg ccgccgggac 480gccgaaaccg cgtcgagcgg cgcgttcgac gcgaaggcgc gtgagcagct gttcgagaaa 540accgttacgc agagcgacgt cgggaagctg aaccgattag tgataccaaa gcagcacgcg 600gagaagcact ttccgttaag cggatccggc ggcggagcct tgccgtgcat ggcggcggct 660gcgggggcga agggaatgtt gctgaacttt gaggacgttg gagggaaagt gtggcggttc 720cgttactcgt attggaacag tagccagagc tacgtgctta ccaaaggatg gagccggttc 780gttaaggaga agaatcttcg agctggtgac gcggttcagt tcttcaagtc gaccggactg 840gaccggcaac tatatataga ctgcaaggcg aggagtggta aggttaacaa taatgctgcc 900ggtttgttta ttccggttgg accggttgtt gagccggttc agatggtacg gcttttcggg 960gtcgaccttt tgaaactacc cgtacccggt tcggatggta ttggggttgg ctgtgacggg 1020aagagaaaag agatggagct gtttgcattt gaatgtagca agaagttaaa agtaattgga 1080gctttgtaa 108981347PRTGlycine max 81Met Ile Gly Val Glu Lys Val Thr Ile Cys Met Arg Ile Glu Val Asn1 5 10 15Thr Glu Lys Gly Arg Arg Ala Leu Met Asp Cys Trp Gln Ile Ser Gly 20 25 30Val His Glu Ser Ser Asp Cys Ser Glu Ile Lys Phe Ala Phe Asp Ala 35 40 45Val Val Lys Arg Ala Arg His Glu Glu Asn Asn Ala Ala Ala Gln Lys 50 55 60Phe Lys Gly Val Val Ser Gln Gln Asn Gly Asn Trp Gly Ala Gln Ile65 70 75 80Tyr Ala His Gln Gln Arg Ile Trp Leu Gly Thr Phe Lys Ser Glu Arg 85 90 95Glu Ala Ala Met Ala Tyr Asp Ser Ala Ser Ile Lys Leu Arg Ser Gly 100 105 110Glu Cys His Arg Asn Phe Pro Trp Asn Asp Gln Thr Val Gln Glu Pro 115 120 125Gln Phe Gln Ser His Tyr Ser Ala Glu Thr Val Leu Asn Met Ile Arg 130 135 140Asp Gly Thr Tyr Pro Ser Lys Phe Ala Thr Phe Leu Lys Thr Arg Gln145 150 155 160Thr Gln Lys Gly Val Ala Lys His Ile Gly Leu Lys Gly Asp Asp Glu 165 170 175Glu Gln Phe Cys Cys Thr Gln Leu Phe Gln Lys Glu Leu Thr Pro Ser 180 185 190Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Lys His Ala Val 195 200 205Ser Tyr Phe Pro Tyr Val Gly Gly Ser Ala Asp Glu Ser Gly Ser Val 210 215 220Asp Val Glu Ala Val Phe Tyr Asp Lys Leu Met Arg Leu Trp Lys Phe225 230 235 240Arg Tyr Cys Tyr Trp Lys Ser Ser Gln Ser Tyr Val Phe Thr Arg Gly 245 250 255Trp Asn Arg Phe Val Lys Asp Lys Lys Leu Lys Ala Lys Asp Val Ile 260 265 270Ala Phe Phe Thr Trp Gly Lys Ser Gly Gly Glu Gly Glu Ala Phe Ala 275 280 285Leu Ile Asp Val Ile Tyr Asn Asn Asn Ala Glu Glu Asp Ser Lys Gly 290 295 300Asp Thr Lys Gln Val Leu Gly Asn Gln Leu Gln Leu Ala Gly Ser Glu305 310 315 320Glu Gly Glu Asp Glu Asp Ala Asn Ile Gly Lys Asp Phe Asn Ala Gln 325 330 335Lys Gly Leu Arg Leu Phe Gly Val Cys Ile Thr 340 345821044DNAGlycine max 82atgattggag ttgagaaagt gacaatttgt atgagaatag aggtgaatac tgaaaaggga 60agaagggctt taatggactg ttggcaaata tcaggagttc atgaaagttc agattgtagc 120gaaatcaaat ttgcattcga cgcagtagta aaacgcgcga ggcatgaaga gaataatgca 180gcagcacaga agttcaaagg cgttgtgtct caacaaaatg ggaactgggg tgcacagata 240tatgcacacc agcagagaat ctggttgggg accttcaaat ctgaaagaga ggctgcaatg 300gcttatgaca gcgccagcat aaaacttaga agcggagagt gccacagaaa ctttccatgg 360aacgaccaaa cagttcaaga gcctcagttc caaagccatt acagcgcaga aacagtgcta 420aacatgatta gagatggcac ctatccatca aaatttgcta catttctcaa aactcgtcaa 480acccaaaaag gcgttgcgaa acacataggt ctgaagggtg atgacgagga acagttttgt 540tgcacccaac tttttcagaa ggaattaaca ccaagtgatg tgggcaagct caacaggctt 600gtcatcccaa agaagcatgc agttagctat tttccttacg ttggtggcag tgctgatgag 660agtggtagtg ttgacgtgga ggctgtgttt tatgacaaac tcatgcgatt gtggaagttc 720cgatactgct attggaagag cagccaaagt tacgtgttca ccagaggctg gaatcggttt 780gtgaaggata agaagttgaa ggctaaagat gtcattgcgt tttttacgtg gggaaaaagt 840ggaggagagg gagaagcttt tgcattgatc gatgtaattt ataataataa tgcagaagaa 900gacagcaagg gagacaccaa acaagttttg ggaaaccaat tacaattagc tggcagtgaa 960gaaggtgaag atgaagatgc aaacattgga aaggatttca atgcacaaaa gggtctgagg 1020ctctttggtg tgtgtatcac ctaa 104483409PRTHordeum vulgare 83Met Glu Phe Thr Ala Thr Ser Ser Arg Phe Ser Lys Gly Glu Glu Glu1 5 10 15Val Glu Glu Glu Gln Glu Glu Ala Ser Met Arg Glu Ile Pro Phe Met 20 25 30Thr Pro Ala Ala Ala Thr Cys Ala Ala Ala Pro Pro Ser Ala Ser Ala 35 40 45Ser Ala Ser Thr Pro Ala Ser Ala Ser Gly Ser Ser Pro Pro Phe Arg 50 55 60Ser Gly Asp Asp Ala Gly Ala Ser Gly Ser Gly Ala Gly Asp Gly Ser65 70 75 80Arg Ser Asn Val Ala Glu Ala Val Glu Lys Glu His Met Phe Asp Lys 85 90 95Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 100 105 110Lys Gln Tyr Ala Glu Lys Tyr Phe Pro Leu Asp Ser Ala Ala Asn Glu 115 120 125Lys Gly Leu Leu Leu Asn Phe Glu Asp Ser Ala Gly Lys Pro Trp Arg 130 135 140Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys145 150 155 160Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr 165 170 175Val Ser Phe Ser Arg Gly Ala Gly Glu Ala Ala Arg His Arg Leu Phe 180 185 190Ile Asp Trp Lys Arg Arg Ala Asp Thr Arg Asp Pro Leu Arg Leu Pro 195 200 205Arg Leu Pro Leu Pro Met Pro Leu Thr Ser His Tyr Ser Pro Trp Gly 210 215 220Leu Gly Ala Gly Ala Arg Gly Phe Phe Met Pro Pro Ser Pro Pro Ala225 230 235 240Thr Leu Tyr Glu His Arg Leu Arg Gln Gly Phe Asp Phe Arg Gly Met 245 250 255Asn Pro Ser Tyr Pro Thr Met Gly Arg Gln Val Ile Leu Phe Gly Ser 260 265 270Ala Ala Arg Met Pro Pro His Gly Pro Ala Pro Leu Leu Val Pro Arg 275 280 285Pro Pro Pro Pro Leu His Phe Thr Val Gln Gln Gln Gly Ser Asp Ala 290 295 300Gly Gly Ser Val Thr Ala Gly Ser Pro Val Val Leu Asp Ser Val Pro305 310 315 320Val Ile Glu Ser Pro Thr Thr Ala Thr Lys Lys Arg Val Arg Leu Phe 325 330 335Gly Val Asn Leu Asp Asn Pro Gln His Pro Gly Asp Gly Gly Gly Glu 340 345 350Ser Ser Asn Tyr Gly Ser Ala Leu Pro Leu Gln Met Pro Ala Ser Ala 355 360 365Trp Arg Pro Arg Asp His Thr Leu Arg Leu Leu Glu Phe Pro Ser His 370 375 380Gly Ala Glu Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser Lys Arg Glu385 390 395 400Ala His Ser Gly Leu Asp Leu Asp Leu 405841230DNAHordeum vulgare 84atggagttta ctgcgacaag cagtaggttt tctaaaggag aggaggaggt ggaggaggag 60caggaggagg cgtcgatgcg cgagatccct ttcatgacgc ccgcggccgc cacctgcgcc 120gcggcgccgc cttctgcttc tgcgtcggcc tcgacacccg cgtcagcgtc tggaagtagc 180cctccctttc gatctgggga tgacgccgga gcgtcgggga gcggggccgg cgacggcagc 240cgcagcaacg tggcggaggc cgtggagaag gagcacatgt tcgacaaagt ggtgacgccg 300agcgacgtgg ggaagcttaa ccggctggtc atccccaagc agtacgccga gaagtacttc 360ccgctggact cggcggccaa cgagaagggc cttctgctca acttcgagga cagcgccggg 420aagccatggc gcttccgcta ttcctactgg aacagcagcc agagctacgt catgaccaaa 480ggctggagcc gcttcgtcaa ggagaagcgc ctcgacgctg gggacaccgt ctccttctcc 540cgcggcgccg gtgaggccgc gcgccaccgc ctcttcatcg actggaagcg ccgagccgac 600accagagacc cgctccgctt gccccgcctc ccgctcccga tgccgctgac gtcgcactac 660agcccgtggg gcctcggcgc cggcgccaga ggattcttca tgcctccctc gccgccagcc 720acgctctacg agcaccgtct ccgtcaaggc ttcgacttcc gcggcatgaa ccccagttac 780cccacaatgg ggagacaggt catccttttc ggctcggccg ccaggatgcc tccgcacgga 840ccagcaccac tcctcgtgcc gcgcccgccg ccgccgctgc acttcacggt gcagcaacaa 900ggcagcgacg ccggcggaag tgtaaccgca ggatccccag tggtgctcga ctcagtgccg 960gtaatcgaaa gccccacgac ggcaacgaag aagcgcgtgc gcttgttcgg cgtgaacttg 1020gacaacccgc agcatcccgg tgatggcggg ggcgaatcga gcaattatgg cagtgcactg 1080ccattgcaga tgcccgcatc agcatggcgg ccaagggacc atacgctgag gctgctcgaa 1140ttcccctcgc acggtgccga ggcgtcgtct ccatcgtcgt cgtcgtcttc caagagggag 1200gcgcattcgg gcttggatct cgatctgtga 123085227PRTHordeum vulgare 85Met Leu Arg Lys His Thr Tyr Phe Asp Glu Leu Ala Gln Ser Lys Arg1 5 10 15Ala Phe Ala Ala Ser Ala Ala Leu Ser Ala Pro Thr Thr Ser Gly Asp 20 25 30Ala Gly Gly Ser Ala Ser Pro Pro Ser Pro Ala Ala Val Arg Glu His 35 40 45Leu Phe Asp Lys Thr Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg 50 55 60Leu Val Ile Pro Lys Gln Asn Ala Glu Lys His Phe Pro Leu Gln Leu65 70 75 80Pro Ala Gly Gly Gly Glu Ser Lys Gly Leu Leu Leu Asn Phe Glu Asp 85 90 95Asp Ala Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 100 105 110Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 115 120 125Gly Leu Gly Ala Gly Asp Val Val Gly Phe Tyr Arg Ser Ala Ala Gly 130 135 140Arg Thr Gly Glu Asp Ser Lys Phe Phe Ile Asp Cys Arg Leu Arg Pro145 150 155 160Asn Thr Asn Thr Ala Ala Glu Ala Asp Pro Val Asp Gln Ser Ser Ala 165 170 175Pro Val Gln Lys Ala Val Arg Leu Phe Gly Val Asp Leu Leu Ala Ala 180 185 190Pro Glu Gln Gly Met Pro Gly Gly Cys Lys Arg Ala Arg Asp Leu Val 195 200 205Lys Pro Pro Pro Pro Lys Val Ala Phe Lys Lys Gln Cys Ile Glu Leu 210 215 220Ala Leu Ala22586684DNAHordeum vulgare 86atgctccgca agcacaccta cttcgacgag ctcgcccaga gcaagcgcgc cttcgccgcg 60tcggccgcgc tctccgcgcc caccacctcg ggcgacgccg gcggcagcgc ctcgccgccc 120tccccggccg ccgtgcgcga gcacctcttc gacaagaccg tcacgcccag cgacgtcggc 180aagctgaaca ggctggtgat accgaagcag aacgccgaga agcacttccc gctgcagctc 240ccggccggcg gcggcgagag caagggcctg ctcctcaact tcgaggacga tgcgggcaag 300gtgtggcggt tccgctactc gtactggaac agcagccaga gctacgtcct caccaagggc 360tggagccgct tcgtgaagga gaagggcctc ggcgccggag acgtcgtcgg gttctaccgc 420tccgccgccg ggaggaccgg cgaagacagc aagttcttca ttgactgcag gctgcggccg 480aacaccaaca ccgccgccga agcagacccc gtggaccagt cgtcggcgcc cgtgcagaag 540gccgtgagac tcttcggcgt cgatcttctc gcggcgccgg agcagggcat gccgggcggg 600tgcaagaggg ccagagactt ggtgaagccg ccgcctccga aagtggcgtt caagaagcaa 660tgcatagagc tggcgctagc gtag 68487160PRTHordeum vulgare 87Met Tyr Cys Ser Arg Gly Arg Ile Asp Pro Ala Glu Glu Gly Gln Val1 5 10 15Met Gly Gly Leu Gly Val Arg Asp Ala Ser Trp Ala Leu Phe Lys Val 20 25 30Leu Glu Gln Ser Asp Val Gln Val Gly Gln Asn Arg Leu Leu Leu Thr 35 40 45Lys Glu Ala Val Trp Gly Gly Pro Ile Pro Lys Leu Phe Pro Glu

Leu 50 55 60Glu Glu Leu Arg Gly Asp Gly Leu Asn Ala Glu Asn Arg Val Ala Val65 70 75 80Lys Ile Leu Asp Ala Asp Gly Cys Glu Gly Asp Ala Asn Phe Arg Tyr 85 90 95Leu Asn Ser Ser Lys Ala Tyr Arg Val Met Gly Pro Gln Trp Ser Arg 100 105 110Leu Val Lys Glu Thr Gly Met Cys Lys Gly Asp Arg Leu Asp Leu Tyr 115 120 125Ala Ala Thr Ala Thr Ala Ala Ser Ser Cys Ser Gly Ala Arg Ala Ala 130 135 140Val Ala Pro Ala Ile Pro Pro Gly Ala Ile Val Lys Ala Ala Gly Phe145 150 155 16088483DNAHordeum vulgare 88atgtattgtt cccgcggccg catcgatccc gcggaagaag ggcaggtgat gggcggcctc 60ggcgtgcgcg acgccagctg ggcgctgttc aaggtgttgg agcagtccga cgtccaggtg 120gggcagaacc ggctgctcct caccaaggag gcggtgtggg gcggccctat ccccaagctt 180ttcccggagc tggaggagct ccgcggcgac ggcctcaacg ccgagaacag ggtcgcggtc 240aagatcctcg acgccgacgg ctgcgagggg gacgccaact tccgctacct caactccagc 300aaggcgtacc gggtcatggg gcctcagtgg agccggctcg tgaaggagac cggcatgtgc 360aagggagacc gcctcgatct gtacgcggca acggcgaccg ctgcctcttc gtgttctgga 420gccagggcgg ctgtggcgcc ggcgatacct cccggagcaa tcgtgaaggc agccgggttc 480taa 48389267PRTHordeum vulgare 89Met Leu Arg Lys His Ile Tyr Pro Asp Glu Leu Ala Gln His Lys Arg1 5 10 15Ala Phe Phe Phe Ala Ala Ala Ser Ser Pro Thr Ser Ser Ser Ser Pro 20 25 30Leu Ala Ser Pro Ala Pro Ser Ala Ala Ala Ala Arg Arg Glu His Leu 35 40 45Phe Asp Lys Thr Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu 50 55 60Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Gln Leu Pro65 70 75 80Ser Ala Ser Ala Ala Val Pro Gly Glu Cys Lys Gly Val Leu Leu Asn 85 90 95Phe Asp Asp Ala Thr Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp 100 105 110Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val 115 120 125Lys Glu Lys Gly Leu His Ala Gly Asp Ala Val Glu Phe Tyr Arg Ala 130 135 140Ala Ser Gly Asn Asn Gln Leu Phe Ile Asp Cys Lys Leu Arg Ser Lys145 150 155 160Ser Thr Thr Thr Thr Thr Ser Val Asn Ser Glu Ala Ala Pro Ser Pro 165 170 175Ala Pro Val Thr Arg Thr Val Arg Leu Phe Gly Val Asp Leu Leu Ile 180 185 190Ala Pro Ala Ala Arg His Ala His Glu His Glu Asp Tyr Gly Met Ala 195 200 205Lys Thr Asn Lys Arg Thr Met Glu Ala Ser Val Ala Ala Pro Thr Pro 210 215 220Ala His Ala Val Trp Lys Lys Arg Cys Val Asp Phe Ala Leu Thr Tyr225 230 235 240Arg Leu Ala Thr Thr Pro Gln Cys Pro Arg Ser Arg Asp Gln Leu Glu 245 250 255Gly Val Gln Ala Ala Gly Ser Thr Phe Ala Leu 260 26590804DNAHordeum vulgare 90atgctgcgca agcacatcta tcccgacgag ctcgcgcagc acaagcgcgc cttcttcttc 60gccgcggcgt cgtcccctac gtcgtcgtcg tcacctctcg cctcgccggc tccttcagcc 120gcggcggcgc ggcgcgagca cctgttcgac aagacggtca cgcccagcga cgtggggaag 180ctgaaccggc tggtgatccc caagcagcac gccgagaagc acttcccgct gcagctccct 240tctgccagcg ccgccgtgcc aggcgagtgc aagggcgtgc tgctcaactt cgatgacgcg 300accggcaagg tgtggaggtt ccggtactcc tactggaaca gcagccagag ctacgtgctc 360accaaggggt ggagccgctt cgtgaaggag aagggccttc acgccggcga cgccgtcgag 420ttctaccgcg ccgcctccgg caacaaccag ctcttcatcg actgcaagct ccggtccaag 480agcaccacga cgacgacctc cgtcaactcg gaggccgccc catcgccggc acccgtgacg 540aggacagtgc gactcttcgg ggtcgacctt ctcatcgcgc cggcggcgag gcacgcgcat 600gagcacgagg actacggcat ggccaagaca aacaagagaa ccatggaggc cagcgtagcg 660gcgcctactc cggcgcacgc ggtgtggaag aagcggtgcg tagacttcgc gctgacctac 720cgacttgcca ccaccccaca gtgcccgagg tcaagagatc aactagaagg agtacaagca 780gctgggagta catttgctct atag 80491357PRTHordeum vulgare 91Met Gly Val Glu Ile Leu Ser Ser Thr Gly Glu His Ser Ser Gln Tyr1 5 10 15Ser Ser Gly Ala Ala Ser Thr Ala Thr Thr Glu Ser Gly Val Gly Gly 20 25 30Arg Pro Pro Thr Ala Pro Ser Leu Pro Val Ser Ile Ala Asp Glu Ser 35 40 45Ala Thr Ser Arg Ser Ala Ser Ala Gln Ser Thr Ser Ser Arg Phe Lys 50 55 60Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu65 70 75 80Arg His Ala Arg Val Trp Leu Gly Thr Phe Pro Asp Glu Asp Ser Ala 85 90 95Ala Arg Ala Tyr Asp Val Ala Ala Leu Arg Tyr Arg Gly Arg Glu Ala 100 105 110Ala Thr Asn Phe Pro Cys Ala Ala Ala Glu Ala Glu Leu Ala Phe Leu 115 120 125Ala Ala His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr 130 135 140Tyr Thr Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Met Gly145 150 155 160Ala Arg Ala Gln Pro Thr Pro Ser Trp Ala Arg Glu Pro Leu Phe Glu 165 170 175Lys Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val 180 185 190Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Lys Arg Thr Pro Glu 195 200 205Thr Thr Thr Thr Thr Gly Lys Gly Val Leu Leu Asn Phe Glu Asp Gly 210 215 220Glu Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln225 230 235 240Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Arg Glu Lys Gly 245 250 255Leu Gly Ala Gly Asp Ser Ile Val Phe Ser Cys Ser Ala Tyr Gly Gln 260 265 270Glu Lys Gln Phe Phe Ile Asp Cys Lys Lys Asn Lys Thr Met Thr Ser 275 280 285Cys Pro Ala Asp Asp Arg Gly Ala Ala Thr Ala Ser Pro Pro Val Ser 290 295 300Glu Pro Thr Lys Gly Glu Gln Val Arg Val Val Arg Leu Phe Gly Val305 310 315 320Asp Ile Ala Gly Glu Lys Arg Gly Arg Ala Ala Pro Val Glu Gln Glu 325 330 335Leu Phe Lys Arg Gln Cys Val Ala His Ser Gln His Ser Pro Ala Leu 340 345 350Gly Ala Phe Val Leu 355921074DNAHordeum vulgare 92atgggggtgg agatcctgag ctcaacgggg gaacactcct cccagtactc ttccggagcc 60gcgtccacgg cgacgacgga gtcaggcgtg ggcggacggc cgccgactgc gccgagccta 120cctgtttcca tcgccgacga gtcggcgacc tcgcggtcgg catcggcgca gtcgacgtcg 180tcgcggttca agggcgtggt gccgcagccc aacgggcggt ggggcgccca gatctacgag 240cgccacgccc gcgtctggct cggcacgttc ccggacgaag actctgcggc gcgcgcctac 300gacgtggccg cgctccggta ccggggccgc gaggccgcca ccaacttccc gtgcgcggcc 360gccgaggcgg agctcgcctt cctggcggca cactccaagg ccgagatcgt cgacatgctc 420cggaagcaca cctacaccga cgagctccgc cagggcctgc ggcgcggccg cggcatgggg 480gcgcgcgcgc agccgacgcc gtcgtgggcg cgggagcccc ttttcgagaa ggccgtgacc 540ccgagcgacg tgggcaagct caaccgcctc gttgtgccga agcagcacgc cgagaagcac 600ttccccctga aacgcacgcc ggagacgaca acgaccaccg gcaagggggt gcttctcaac 660ttcgaggatg gcgaggggaa agtgtggagg ttccggtact cgtattggaa cagcagccag 720agctacgtgc tcaccaaggg atggagccgc ttcgttcggg agaagggcct cggtgccggc 780gactccatcg tgttctcctg ctcggcgtac ggtcaggaga agcagttctt catcgactgc 840aagaagaaca agacgatgac gagctgcccc gccgatgacc gcggcgccgc aacagcgtcg 900ccgccagtgt cagagccaac aaaaggagaa caagtccgtg ttgtgaggct gttcggcgtc 960gacatcgccg gagagaagag ggggcgagcg gcgccggtgg agcaggagtt gttcaagagg 1020caatgcgtgg cacacagcca gcactctcca gccctaggtg ccttcgtctt atag 107493348PRTHordeum vulgare 93Met Gly Val Glu Ile Leu Ser Ser Met Val Glu His Ser Phe Gln Tyr1 5 10 15Ser Ser Gly Ala Ser Ser Ala Thr Ala Glu Ser Gly Ala Val Gly Thr 20 25 30Pro Pro Arg His Leu Ser Leu Pro Val Ala Ile Ala Asp Glu Ser Leu 35 40 45Thr Ser Arg Ser Ala Ser Ser Arg Phe Lys Gly Val Val Pro Gln Pro 50 55 60Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Arg His Ala Arg Val Trp65 70 75 80Leu Gly Thr Phe Pro Asp Gln Asp Ser Ala Ala Arg Ala Tyr Asp Val 85 90 95Ala Ser Leu Arg Tyr Arg Gly Gly Asp Ala Ala Phe Asn Phe Pro Cys 100 105 110Val Val Val Glu Ala Glu Leu Ala Phe Leu Ala Ala His Ser Lys Ala 115 120 125Glu Ile Val Asp Met Leu Arg Lys Gln Thr Tyr Ala Asp Glu Leu Arg 130 135 140Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Val Arg Ala Gln Pro Met145 150 155 160Pro Ser Trp Ala Arg Val Pro Leu Phe Glu Lys Ala Val Thr Pro Ser 165 170 175Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln His Ala Glu 180 185 190Lys His Phe Pro Leu Lys Arg Ser Pro Glu Thr Thr Thr Thr Thr Gly 195 200 205Asn Gly Val Leu Leu Asn Phe Glu Asp Gly Gln Gly Lys Val Trp Arg 210 215 220Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys225 230 235 240Gly Trp Ser Arg Phe Val Arg Glu Lys Gly Leu Gly Ala Gly Asp Ser 245 250 255Ile Met Phe Ser Cys Ser Ala Tyr Gly Gln Glu Lys Gln Phe Phe Ile 260 265 270Asp Cys Lys Lys Asn Thr Thr Val Asn Gly Gly Lys Ser Ala Ser Pro 275 280 285Leu Gln Val Met Glu Ile Ala Lys Ala Glu Gln Val Arg Val Val Arg 290 295 300Leu Phe Gly Val Asp Ile Ala Gly Val Lys Arg Glu Arg Ala Ala Thr305 310 315 320Ala Glu Gln Gly Pro Gln Gly Trp Phe Lys Arg Gln Cys Met Ala His 325 330 335Gly Gln His Ser Pro Ala Leu Gly Asp Phe Ala Leu 340 345941047DNAHordeum vulgare 94atgggggtgg agatcctgag ctccatggtg gagcactcct tccagtactc ttcgggcgcg 60tcctcggcca ccgcggagtc aggcgccgtc ggaacaccgc cgaggcatct gagcctacct 120gtcgccatcg ccgacgagtc cctgacctca cggtcggcgt cgtctcggtt caagggcgtg 180gtgccgcagc ccaacgggcg gtggggcgcc cagatctacg agcgccacgc tcgcgtctgg 240ctcggcacgt tcccagacca ggactcggcg gcgcgcgcct acgacgttgc ctcgctcagg 300taccgcggcg gcgacgccgc cttcaacttc ccgtgcgtgg tggtggaggc ggagctcgcc 360ttcctggcgg cgcactccaa ggctgagatc gttgacatgc tccggaagca gacctacgcc 420gatgaactcc gccagggact acggcgcggc cgtggcatgg gggtgcgcgc gcagccgatg 480ccgtcgtggg cgcgggttcc ccttttcgag aaggccgtga cccctagcga tgtcggcaag 540ctcaatcgcc tggtggtgcc gaagcagcac gccgagaagc acttccccct gaagcgcagc 600ccggagacga cgaccaccac cggcaacggc gtactgctca actttgagga cggccaggga 660aaagtgtgga ggttccggta ctcatattgg aacagcagcc agagctacgt gctcaccaaa 720ggctggagcc gcttcgtccg ggagaagggc ctcggcgccg gtgactccat catgttctcc 780tgctcggcgt acgggcagga gaagcagttc ttcatcgact gcaagaagaa cacgaccgtg 840aacggaggca aatcggcgtc gccgctgcag gtgatggaga ttgccaaagc agaacaagtc 900cgcgtcgtta gactgttcgg tgtcgacatc gccggggtga agagggagcg agcggcgacg 960gcggagcaag gcccgcaggg gtggttcaag aggcaatgca tggcacacgg ccagcactct 1020cctgccctag gtgacttcgc cttatag 104795362PRTHordeum vulgare 95Met Gly Met Glu Ile Leu Ser Ser Thr Val Glu His Cys Ser Gln Tyr1 5 10 15Ser Ser Ser Ala Ser Thr Ala Thr Thr Glu Ser Gly Ala Ala Gly Arg 20 25 30Ser Thr Thr Ala Leu Ser Leu Pro Val Ala Ile Thr Asp Glu Ser Val 35 40 45Thr Ser Arg Ser Ala Ser Ala Gln Pro Ala Ser Ser Arg Phe Lys Gly 50 55 60Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ser Gln Ile Tyr Glu Arg65 70 75 80His Ala Arg Val Trp Leu Gly Thr Phe Pro Asp Gln Asp Ser Ala Ala 85 90 95Arg Ala Tyr Asp Val Ala Ser Leu Arg Tyr Arg Gly Arg Asp Ala Ala 100 105 110Thr Asn Phe Pro Cys Ala Ala Ala Glu Ala Glu Leu Ala Phe Leu Thr 115 120 125Ala His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Ala Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Ala145 150 155 160Arg Ala Gln Pro Thr Pro Ser Trp Ala Arg Val Pro Leu Phe Glu Lys 165 170 175Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro 180 185 190Lys Gln His Ala Glu Lys His Phe Pro Leu Lys Cys Thr Ala Glu Thr 195 200 205Thr Thr Thr Thr Gly Asn Gly Val Leu Leu Asn Phe Glu Asp Gly Glu 210 215 220Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser225 230 235 240Tyr Val Leu Thr Lys Gly Trp Ser Ser Phe Val Arg Glu Lys Gly Leu 245 250 255Gly Ala Gly Asp Ser Ile Val Phe Ser Ser Ser Ala Tyr Gly Gln Glu 260 265 270Lys Gln Leu Phe Ile Asn Cys Lys Lys Asn Thr Thr Met Asn Gly Gly 275 280 285Lys Thr Ala Leu Pro Leu Pro Val Val Glu Thr Ala Lys Gly Glu Gln 290 295 300Asp His Val Val Lys Leu Phe Gly Val Asp Ile Ala Gly Val Lys Arg305 310 315 320Val Arg Ala Ala Thr Gly Glu Leu Gly Pro Pro Glu Leu Phe Lys Arg 325 330 335Gln Ser Val Ala His Gly Cys Gly Arg Met Asn Tyr Ile Cys Tyr Ser 340 345 350Ile Gly Thr Ile Gly Pro Leu Met Leu Asn 355 360961089DNAHordeum vulgare 96atggggatgg aaatcctgag ctccacggtg gagcactgct cccagtactc ttccagcgcg 60tccacggcca caacggagtc aggcgccgcc ggaagatcga cgacggctct gagcctacca 120gttgccatca ccgacgagtc cgttacctcg cggtcggcat cggcgcagcc ggcgtcatca 180cggttcaagg gcgtggtgcc gcagcccaac gggcggtggg gctcccagat ctacgagcgc 240cacgctcgcg tctggctcgg caccttcccg gatcaggact cggcggcgcg tgcctacgac 300gttgcctcgc tcaggtaccg gggccgcgat gccgccacca acttcccgtg cgccgctgcg 360gaagcggagc tcgccttcct gaccgcgcac tccaaggccg agatcgtcga catgctccgg 420aagcacacct acgccgacga actccgccag ggcctgcggc gcggccgcgg catgggtgcg 480cgcgcgcagc cgacgccgtc gtgggcgcgg gttccccttt tcgagaaggc tgtgacccct 540agcgatgtcg gcaagctcaa tcgcctggtg gtgccgaagc agcacgccga gaagcacttc 600cccctgaagt gcaccgcaga gacgacgacc accaccggca acggcgtgct gctaaacttc 660gaggatggtg aggggaaggt gtggaggttc cggtactcgt attggaacag tagccagagc 720tacgtgctca ccaaaggctg gagcagcttc gtccgggaga agggcctcgg cgcaggcgac 780tccatcgtct tctcctcctc ggcgtacggg caggagaagc agttattcat caactgcaaa 840aagaacacga ctatgaacgg cggcaaaaca gcgttgccgc tgccagtggt ggagactgcc 900aaaggagaac aagaccacgt cgttaagttg ttcggtgttg acatcgccgg tgtgaagagg 960gtgcgagcgg cgacggggga gctaggcccg ccggagttgt tcaagagaca atccgtggca 1020cacggatgcg gaaggatgaa ctacatttgc tactccatag ggacaatagg acctcttatg 1080ctcaactga 108997308PRTHordeum vulgare 97Met Ala Ser Ser Lys Pro Thr Asn Pro Glu Val Asp Asn Asp Met Glu1 5 10 15Cys Ser Ser Pro Glu Ser Gly Ala Glu Asp Ala Val Glu Ser Ser Ser 20 25 30Pro Val Ala Ala Pro Ser Ser Arg Phe Lys Gly Val Val Pro Gln Pro 35 40 45Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Lys His Ser Arg Val Trp 50 55 60Leu Gly Thr Phe Gly Asp Glu Glu Ala Ala Ala Cys Ala Tyr Asp Val65 70 75 80Ala Ala Leu Arg Phe Arg Gly Arg Asp Ala Val Thr Asn His Gln Arg 85 90 95Leu Pro Ala Ala Glu Gly Ala Gly Trp Ser Ser Thr Ser Glu Leu Ala 100 105 110Phe Leu Ala Asp His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys 115 120 125His Thr Tyr Asp Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly His Gly 130 135 140Arg Ala Gln Pro Thr Pro Ala Trp Ala Arg Glu Phe Leu Phe Glu Lys145 150 155 160Ala Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro 165 170 175Lys Gln His Ala Glu Lys His Phe Pro Pro Thr Thr Ala Ala Ala Ala 180 185 190Gly Ser Asp Gly Lys Gly Leu Leu Leu Asn Phe Glu Asp Gly Gln Gly 195 200 205Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr 210 215 220Val Leu Thr Lys Gly Trp Ser Arg Phe Val Gln Glu

Lys Gly Leu Cys225 230 235 240Ala Gly Asp Thr Val Thr Phe Ser Arg Ser Ala Tyr Val Met Asn Asp 245 250 255Thr Asp Glu Gln Leu Phe Ile Asp Tyr Lys Gln Ser Ser Lys Asn Asp 260 265 270Glu Ala Ala Asp Val Ala Thr Ala Asp Glu Asn Glu Ala Gly His Val 275 280 285Ala Val Lys Leu Phe Gly Val Asp Ile Gly Trp Ala Gly Met Ala Gly 290 295 300Ser Ser Gly Gly30598927DNAHordeum vulgare 98atggcgtcta gcaagccgac aaaccccgag gtagacaatg acatggagtg ctcctccccg 60gaatcgggtg ccgaggacgc cgtggagtcg tcgtcgccgg tggcagcgcc atcttcgcgg 120ttcaagggcg tcgtgccgca gcctaacggg cgctggggcg cgcagatcta cgagaagcac 180tcgcgggtgt ggcttggcac gttcggggac gaggaagccg ccgcgtgcgc ctacgacgtg 240gccgcgctcc gcttccgcgg ccgcgacgcc gtcaccaacc accagcgcct gccggcggcg 300gagggggccg gctggtcgtc cacgagcgag ctcgccttcc tcgccgacca ctccaaggcc 360gagatcgtcg acatgctccg gaagcacacc tacgacgacg agctccggca gggcctgcgc 420cgcggccacg ggcgcgcgca gcccacgccg gcgtgggcgc gagagttcct cttcgagaag 480gccctgaccc cgagcgacgt cggcaagctc aaccgcctgg tcgttccgaa gcagcacgcc 540gagaagcact tccccccgac gacggcggcg gccgccggaa gcgacggcaa gggcttgctg 600ctcaacttcg aggacggcca agggaaggtg tggaggttcc ggtactcata ctggaacagc 660agccagagct acgtgctcac caagggctgg agccgcttcg tccaagaaaa gggcctctgc 720gccggcgaca ccgtgacgtt ctcccggtcg gcgtacgtga tgaatgacac ggatgagcag 780ctcttcatcg actacaagca gagtagcaag aacgacgaag cggccgacgt agccactgcc 840gatgagaatg aggccggcca tgtcgccgtg aagctcttcg gggtcgacat tggctgggct 900gggatggcgg gatcatcagg tgggtga 927991279PRTHordeum vulgare 99Met Leu Phe Asp Ser Ser Val Ser Ala Ser Leu Gly Thr Met Arg Pro1 5 10 15Leu Val Lys Lys Leu Asp Met Leu Leu Ala Pro Ala Arg Gly Tyr Ser 20 25 30Thr Leu Cys Lys Arg Ile Lys Glu Val Met His Leu Leu Lys His Asp 35 40 45Val Glu Glu Ile Ser Ser Tyr Leu Asp Glu Leu Thr Glu Val Glu Asp 50 55 60Pro Pro Pro Met Ala Lys Cys Trp Met Asn Glu Ala Arg Asp Leu Ser65 70 75 80Tyr Asp Met Glu Asp Tyr Ile Asp Ser Leu Leu Phe Val Pro Pro Gly 85 90 95His Phe Ile Lys Lys Lys Lys Lys Lys Lys Lys Lys Gly Lys Lys Lys 100 105 110Met Val Ile Lys Lys Arg Leu Lys Trp Cys Lys Gln Ile Val Phe Thr 115 120 125Lys Gln Val Ser Asp His Gly Ile Lys Thr Ser Lys Ile Ile His Val 130 135 140Asn Val Pro Arg Leu Pro Asn Lys Pro Lys Val Ala Lys Ile Ile Leu145 150 155 160Gln Phe Arg Ile Tyr Val Gln Glu Ala Ile Glu Arg Tyr Asp Lys Tyr 165 170 175Arg Leu His His Cys Ser Thr Leu Arg Arg Arg Leu Leu Ser Thr Gly 180 185 190Ser Met Leu Ser Val Pro Ile Pro Tyr Glu Glu Ala Ala Gln Ile Val 195 200 205Thr Asp Gly Arg Met Asn Glu Phe Ile Ser Ser Leu Ala Ala Asn Asn 210 215 220Ala Ala Asp Gln Gln Gln Leu Lys Val Val Ser Val Leu Gly Ser Gly225 230 235 240Cys Leu Gly Lys Thr Thr Leu Ala Asn Val Leu Tyr Asp Arg Ile Gly 245 250 255Met Gln Phe Glu Cys Arg Ala Phe Ile Arg Val Ser Lys Lys Pro Asp 260 265 270Met Lys Arg Leu Phe Arg Asp Leu Leu Ser Gln Phe His Gln Lys Gln 275 280 285Pro Leu Pro Thr Ser Cys Asn Glu Leu Gly Ile Ser Asp Asn Ile Ile 290 295 300Lys His Leu Gln Asp Lys Arg Tyr Leu Ile Val Ile Asp Asp Leu Trp305 310 315 320Asp Leu Ser Val Trp Asp Ile Ile Lys Tyr Ala Phe Pro Lys Gly Asn 325 330 335His Gly Ser Arg Ile Ile Ile Thr Thr Gln Ile Glu Asp Val Ala Leu 340 345 350Thr Cys Cys Cys Asp His Ser Glu His Val Phe Glu Met Lys Pro Leu 355 360 365Asn Ile Gly His Ser Arg Glu Leu Phe Phe Asn Arg Leu Phe Gly Ser 370 375 380Glu Ser Asp Cys Leu Glu Glu Phe Lys Arg Val Ser Asn Glu Ile Val385 390 395 400Asp Ile Cys Gly Gly Leu Pro Leu Ala Thr Ile Asn Ile Ala Ser His 405 410 415Leu Ala Asn Gln Glu Thr Glu Val Ser Leu Asp Leu Leu Thr Asp Thr 420 425 430Arg Asp Leu Leu Arg Ser Cys Leu Trp Ser Asn Ser Thr Ser Glu Arg 435 440 445Thr Lys Gln Val Leu Asn Leu Ser Tyr Ser Asn Leu Pro Asp Tyr Leu 450 455 460Lys Thr Cys Leu Leu Tyr Leu His Met Tyr Pro Val Gly Ser Ile Ile465 470 475 480Trp Lys Asp Asp Leu Val Lys Gln Leu Val Ala Glu Gly Phe Ile Ala 485 490 495Thr Arg Glu Gly Lys Asp Gln Asp Gln Glu Met Ile Glu Lys Ala Ala 500 505 510Gly Leu Cys Phe Asp Ala Leu Ile Asp Arg Arg Phe Ile Gln Pro Ile 515 520 525Tyr Thr Lys Tyr Asn Asn Lys Val Leu Ser Cys Thr Val His Glu Val 530 535 540Val His Asp Leu Ile Ala Gln Lys Ser Ala Glu Glu Asn Phe Ile Val545 550 555 560Val Ala Asp His Asn Arg Lys Asn Ile Ala Leu Ser His Lys Val Arg 565 570 575Arg Leu Ser Leu Ile Phe Gly Asp Thr Ile Tyr Ala Lys Thr Pro Ala 580 585 590Asn Ile Thr Lys Ser Gln Ile Arg Ser Phe Arg Phe Phe Gly Leu Phe 595 600 605Glu Cys Met Pro Cys Ile Thr Glu Phe Lys Val Leu Arg Val Leu Asn 610 615 620Leu Gln Leu Ser Gly His Arg Gly Asp Asn Asp Pro Ile Asp Leu Thr625 630 635 640Gly Ile Ser Glu Leu Phe Gln Leu Arg Tyr Leu Lys Ile Thr Ser Asp 645 650 655Val Cys Ile Lys Leu Pro Asn Gln Met Gln Lys Leu Gln Tyr Leu Glu 660 665 670Thr Leu Asp Ile Met Asp Ala Pro Arg Val Thr Ala Val Pro Trp Asp 675 680 685Ile Ile Asn Leu Pro His Leu Leu His Leu Thr Leu Pro Val Asp Thr 690 695 700Tyr Leu Leu Asp Trp Ile Ser Ser Met Thr Asp Ser Val Ile Ser Leu705 710 715 720Trp Thr Leu Gly Lys Leu Asn Tyr Leu Gln His Leu His Leu Thr Ser 725 730 735Ser Ser Thr Arg Pro Ser Tyr His Leu Glu Arg Ser Val Glu Ala Leu 740 745 750Gly Tyr Leu Ile Gly Gly His Gly Lys Leu Lys Thr Ile Val Val Ala 755 760 765His Val Ser Ser Ala Gln Asn Thr Val Val Arg Gly Ala Pro Glu Val 770 775 780Thr Ile Ser Trp Asp Arg Met Ser Pro Pro Pro Leu Leu Gln Arg Phe785 790 795 800Glu Cys Pro His Ser Cys Phe Ile Phe Tyr Arg Ile Pro Lys Trp Val 805 810 815Thr Glu Leu Gly Asn Leu Cys Ile Leu Lys Ile Ala Val Lys Glu Leu 820 825 830His Met Ile Cys Leu Gly Thr Leu Arg Gly Leu His Ala Leu Thr Asp 835 840 845Leu Ser Leu Tyr Val Glu Thr Ala Pro Ile Asp Lys Ile Ile Phe Asp 850 855 860Lys Ala Gly Phe Ser Val Leu Lys Tyr Cys Lys Leu Arg Phe Ala Ala865 870 875 880Gly Ile Ala Trp Leu Lys Phe Glu Ala Asp Ala Met Pro Ser Leu Trp 885 890 895Lys Leu Met Leu Val Phe Asn Ala Ile Pro Arg Met Asp Gln Asn Leu 900 905 910Val Phe Phe His His Ser Arg Pro Ala Met His Gln Arg Gly Gly Ala 915 920 925Val Ile Ile Val Glu His Met Pro Gly Leu Arg Val Ile Ser Ala Lys 930 935 940Phe Gly Gly Ala Ala Ser Asp Leu Glu Tyr Ala Ser Arg Thr Val Val945 950 955 960Ser Asn His Pro Ser Asn Pro Thr Ile Asn Met Gln Leu Val Cys Tyr 965 970 975Ser Ser Asn Gly Lys Arg Ser Arg Lys Arg Lys Gln Gln Pro Tyr Asp 980 985 990Val Val Lys Gly Gln Pro Asp Glu Tyr Ala Lys Arg Leu Glu Arg Pro 995 1000 1005Ala Glu Lys Arg Ile Ser Thr Pro Thr Lys Ser Ser Leu Arg Leu 1010 1015 1020His Val Pro Glu Ile Thr Pro Lys Pro Met Gln Ile Thr Asp Asn 1025 1030 1035Asn Val Gln Arg Arg Glu His Met Phe Asp Thr Val Leu Thr Arg 1040 1045 1050Gly Asp Val Gly Met Leu Asn Arg Leu Val Val Pro Lys Lys His 1055 1060 1065Ala Glu Lys Tyr Phe Pro Leu Asp Ser Ser Ser Thr Arg Thr Ser 1070 1075 1080Lys Ala Ile Val Leu Ser Phe Glu Asp Pro Ala Gly Lys Ser Trp 1085 1090 1095Phe Phe His Tyr Ser Tyr Arg Ser Ser Ser Gln Asn Tyr Val Met 1100 1105 1110Phe Lys Gly Trp Thr Gly Phe Val Lys Glu Lys Phe Leu Glu Ala 1115 1120 1125Gly Asp Thr Val Ser Phe Ser Arg Gly Val Gly Glu Ala Thr Arg 1130 1135 1140Gly Arg Leu Phe Ile Asp Cys Gln Asn Glu Gln Arg Tyr Met Phe 1145 1150 1155Glu Arg Val Leu Thr Ala Ser Asp Met Glu Ser Asp Gly Cys Ser 1160 1165 1170Leu Met Val Pro Val Asn Leu Val Trp Pro His Pro Gly Leu Arg 1175 1180 1185Lys Thr Ile Lys Gly Arg His Ala Val Leu Gln Phe Glu Asp Gly 1190 1195 1200Ser Gly Asn Gly Lys Val Trp Pro Phe Gln Phe Glu Ala Ser Gly 1205 1210 1215Gln Tyr Tyr Leu Met Lys Gly Leu Asn Tyr Phe Val Asn Asp Arg 1220 1225 1230Asp Leu Ala Ala Gly Tyr Thr Val Ser Phe Tyr Arg Ala Gly Thr 1235 1240 1245Arg Leu Phe Val Asp Ser Gly Arg Lys Asp Asp Lys Val Ala Leu 1250 1255 1260Gly Thr Arg Ser Arg Glu Arg Ile Tyr Pro Lys Ile Val Arg Ser 1265 1270 1275Gln1003840DNABrassica rapa 100atgttgtttg atagttcagt gagtgcttcg ttgggcacca tgagaccact tgtcaagaag 60ctcgacatgc tgctagctcc tgctcgggga tacagtacct tgtgcaagag gatcaaggaa 120gtgatgcacc ttctcaaaca tgatgttgaa gagataagct cctaccttga tgaacttaca 180gaggtggagg accctccacc aatggccaag tgctggatga acgaggcacg cgacctgtct 240tatgatatgg aggattacat tgatagcttg ttatttgtgc cacctggcca tttcatcaag 300aagaagaaga agaagaagaa gaagggaaag aagaagatgg tgataaagaa gaggctcaag 360tggtgcaaac agatcgtatt cacaaagcaa gtgtcagacc atggtatcaa gaccagtaaa 420atcattcatg ttaatgtccc tcgtcttccc aataagccca aggttgcaaa aataatatta 480cagttcagga tctatgtcca ggaggctatt gaacggtatg acaagtatag gcttcaccat 540tgcagcacct tgaggcgtag attgttgtcc actggtagta tgctttcagt gccaataccc 600tatgaagaag ctgcccaaat tgtaactgat ggccggatga atgagtttat cagctcactg 660gctgctaata atgcagcaga tcagcagcag ctcaaggtgg tatctgttct tggatctggg 720tgtctaggta aaactacgct tgcgaatgtg ttgtacgaca gaattgggat gcaattcgaa 780tgcagagctt tcattcgagt gtccaaaaag cctgatatga agagactttt ccgtgacttg 840ctctcgcaat tccaccagaa gcagccactg cctaccagtt gtaatgagct tggcataagt 900gacaatatca tcaaacatct gcaagataaa aggtatctaa ttgttattga tgatttgtgg 960gatttatcag tatgggatat tattaaatat gcttttccaa agggaaacca tggaagcaga 1020ataataataa ctacacagat tgaagatgtt gcattaactt gttgctgtga tcactcggag 1080catgttttcg agatgaaacc tctcaacatt ggtcactcaa gagagctatt ttttaataga 1140ctttttggtt ctgaaagtga ctgtcttgaa gaattcaaac gagtttcaaa cgaaattgtt 1200gatatatgtg gtggtttacc gctagcaaca atcaacatag ctagtcattt ggcaaaccag 1260gagacagaag tatcattgga tttgctaaca gacacacgtg atttgttgag gtcctgtttg 1320tggtcaaatt ctacttcaga aagaacaaaa caagtactga acctcagcta cagtaatctt 1380cctgattatc tgaagacatg tttgctgtat cttcatatgt atccagtggg ctccataatc 1440tggaaggatg atctggtgaa gcaattggtg gctgaagggt ttattgctac aagagaaggg 1500aaagaccaag accaagaaat gatagagaaa gctgcaggac tctgtttcga tgcacttatt 1560gatagaagat tcatccagcc tatatatacc aagtacaaca ataaggtgtt gtcctgcacg 1620gttcatgagg tggtacatga tcttattgcc caaaagtctg ctgaagagaa tttcattgtg 1680gtagcagacc acaatcgaaa gaatatagca ctttctcata aggttcgtcg actatctctc 1740atctttggcg acacaatata tgccaagaca ccagcaaaca tcacaaagtc acaaattcgg 1800tcattcagat tttttggatt attcgagtgt atgccttgta ttacagagtt caaggttctc 1860cgtgttctaa accttcaact atctggtcat cgtggggaca atgaccctat agacctcact 1920gggatttcag aactgtttca gctgagatat ttaaagatta caagtgatgt gtgcataaaa 1980ctaccaaatc aaatgcaaaa actgcaatat ttggaaacgt tggacattat ggatgcacca 2040agagtcactg ctgttccatg ggatattata aatctcccac acctgttgca cctgactctt 2100cctgttgata catatctgct ggattggatt agcagcatga ctgactccgt catcagtctg 2160tggacccttg gcaagctgaa ctacctgcag catcttcatc ttactagttc ttctacacgt 2220ccttcatacc atctggagag aagtgtggag gctctgggtt atttgatcgg aggacatggc 2280aagctgaaaa ctatagtagt cgctcatgtc tcctctgctc aaaatactgt ggttcgtggc 2340gccccagaag taaccatttc atgggatcgt atgtcacctc ccccccttct ccagagattc 2400gaatgcccac acagctgctt catattttac cgaattccta agtgggttac agaacttggc 2460aacctgtgca ttttgaagat tgcagtgaag gagcttcata tgatttgtct tggtactctc 2520agaggattgc atgccctcac tgatctgtcg ctgtatgtgg agacagcgcc cattgacaag 2580atcatctttg acaaggccgg gttctcagtt ctcaagtact gcaaattgcg cttcgcggct 2640ggtatagctt ggctgaaatt tgaggctgat gcaatgccta gtctatggaa actgatgcta 2700gttttcaacg ccatcccacg aatggaccaa aatcttgttt tctttcacca cagccgaccg 2760gcgatgcatc aacgtggtgg tgcagtaatc attgtcgagc atatgccagg gcttagagtg 2820atctccgcaa aatttggggg cgcagcttct gatctagagt atgcttcgag gaccgtcgtt 2880agtaaccatc caagcaatcc tacaatcaac atgcaattgg tgtgttatag ttccaatggt 2940aagagaagca gaaaaaggaa acaacaacct tacgacgttg tgaagggaca accagatgaa 3000tacgccaaga gattggagag accagctgag aaaaggattt caacgccgac aaagtcttct 3060ttgcgtctgc atgttccaga aattacacca aaacctatgc agattacaga caacaatgtt 3120cagaggaggg agcacatgtt cgatacggtt ctgactcggg gggacgtggg gatgctgaac 3180cggctggtgg taccgaagaa gcacgcggag aagtacttcc cgctggacag ttcctccacc 3240cgcaccagca aggccatcgt actcagcttt gaggaccctg ctgggaagtc atggttcttc 3300cactactcct accggagcag cagccagaac tacgtcatgt tcaaggggtg gactggcttc 3360gtcaaggaga agtttctcga agccggcgac accgtctcct tcagccgcgg cgtcggggag 3420gccacgaggg ggaggctctt catcgactgt caaaatgagc agaggtacat gttcgagcga 3480gtgctgacgg cgagtgatat ggagtcggat ggctgctcgc tgatggtccc agtgaacttg 3540gtgtggccgc accccggcct ccgcaagacg atcaagggga ggcacgccgt gctgcagttt 3600gaggacggca gcggcaacgg gaaggtgtgg ccatttcagt ttgaggcctc cggccaatac 3660tatctcatga agggcttgaa ctactttgtt aacgaccgcg accttgcggc tggctatacc 3720gtctccttct accgcgccgg cacgcggttg ttcgtcgact ccgggcgtaa agatgacaaa 3780gtagccttgg gaaccagaag ccgcgaaagg atctatccta agatcgtgcg gtcgcagtag 3840101264PRTBrassica rapa 101Met Ser Gly Asn His Tyr Ser Arg Asp Ile His His Asn Thr Pro Ser1 5 10 15Val His His His Gln Asn Tyr Ala Val Val Asp Arg Glu Tyr Leu Phe 20 25 30Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val 35 40 45Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Asn Asn Ala Gly 50 55 60Asp Asp Val Ala Ala Ala Glu Thr Thr Glu Lys Gly Met Leu Leu Thr65 70 75 80Phe Glu Asp Glu Ser Gly Lys Cys Trp Lys Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val 100 105 110Lys Asp Lys His Leu His Ala Gly Asp Val Val Phe Phe Gln Arg His 115 120 125Arg Phe Asp Leu His Arg Val Phe Ile Gly Trp Arg Lys Arg Gly Glu 130 135 140Val Ser Ser Pro Thr Ala Val Ser Val Val Ser Gln Glu Ala Arg Val145 150 155 160Asn Thr Thr Ala Tyr Trp Ser Gly Leu Thr Thr Pro Tyr Arg Gln Val 165 170 175His Ala Ser Thr Ser Ser Tyr Pro Asn Ile His Gln Glu Tyr Ser His 180 185 190Tyr Gly Ala Val Ala Glu Ile Pro Thr Val Val Thr Gly Ser Ser Arg 195 200 205Thr Val Arg Leu Phe Gly Val Asn Leu Glu Cys His Gly Asp Val Val 210 215 220Glu Thr Pro Pro Cys Pro Asp Gly Tyr Asn Gly Gln His Phe Tyr Tyr225 230 235 240Tyr Ser Thr Pro Asp Pro Met Asn Ile Ser Phe Ala Gly Glu Ala Met 245 250 255Glu Gln Val Gly Asp Gly Arg Arg 260102258PRTBrassica rapa 102Met Ser Val Asn His Tyr Ser Asn Thr Leu Ser Ser His Asn His His1 5 10 15Asn Glu His Lys Glu Ser Leu Phe Glu Lys Ser Leu Thr Pro Ser Asp 20 25 30Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg 35 40

45Tyr Leu Pro Leu Asn Asn Cys Gly Gly Gly Gly Asp Val Thr Ala Glu 50 55 60Ser Thr Glu Lys Gly Val Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys65 70 75 80Ser Trp Lys Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 85 90 95Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys His Leu Asn Ala 100 105 110Gly Asp Val Val Leu Phe Gln Arg His Arg Phe Asp Ile His Arg Leu 115 120 125Phe Ile Gly Trp Arg Arg Arg Gly Glu Ala Ser Ser Ser Ser Ala Val 130 135 140Ser Ala Val Thr Gln Asp Pro Arg Ala Asn Thr Thr Ala Tyr Trp Asn145 150 155 160Gly Leu Thr Thr Pro Tyr Arg Gln Val His Ala Ser Thr Ser Ser Tyr 165 170 175Pro Asn Asn Ile His Gln Glu Tyr Ser His Tyr Gly Pro Val Ala Glu 180 185 190Thr Pro Thr Val Ala Ala Gly Ser Ser Lys Thr Val Arg Leu Phe Gly 195 200 205Val Asn Leu Glu Cys His Ser Asp Val Val Glu Pro Pro Pro Cys Pro 210 215 220Asp Ala Tyr Asn Gly Gln His Ile Tyr Tyr Tyr Ser Thr Pro His Pro225 230 235 240Met Asn Ile Ser Phe Ala Gly Glu Ala Met Glu Gln Val Gly Asp Gly 245 250 255Arg Gly103777DNABrassica rapa 103atgtcagtca accattactc aaacactctc tcgtcgcaca atcaccacaa cgaacataaa 60gagtctttgt tcgagaagtc actcacgcca agcgatgttg gaaagctaaa ccgtttagtc 120ataccaaaac aacacgccga gagatacctc cctctcaata attgcggcgg cggcggcgac 180gtgacggcgg agtcgacgga gaaaggggtg cttctcagct tcgaggacga gtcgggaaaa 240tcttggaaat tcagatactc atattggaac agtagtcaaa gctacgtgtt gaccaaagga 300tggagcaggt acgtcaaaga caagcacctc aacgcagggg acgtcgtttt atttcaacgg 360caccgttttg atattcatag actcttcatt ggctggagga gacgcggaga ggcttcttcc 420tcttccgccg tttccgccgt gactcaagat cctcgagcta acacgacggc gtactggaac 480ggtttgacta caccttatcg tcaagtacac gcgtcaacta gttcttaccc taacaacatc 540caccaagagt attcacatta tggccctgtt gctgagacac cgacggtagc tgcagggagc 600tcgaagacgg tgaggctatt tggagttaac ctcgaatgtc acagtgacgt tgtggagcca 660ccaccgtgtc ctgacgccta caacggccaa cacatttact attactcaac tccacatccc 720atgaatatct catttgctgg agaagcaatg gagcaggtag gagatggacg aggttga 777104267PRTBrassica rapa 104Met Ser Val Asn His Tyr Ser Thr Asp His His Gln Val His His His1 5 10 15His Thr Leu Phe Leu Gln Asn Leu His Thr Thr Asp Thr Ser Glu Pro 20 25 30Thr Thr Thr Ala Ala Thr Ser Leu Arg Glu Asp Gln Lys Glu Tyr Leu 35 40 45Phe Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu 50 55 60Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Thr Ile65 70 75 80Ile Ser Asn Asn Ala Glu Glu Lys Gly Met Leu Leu Ser Phe Glu Asp 85 90 95Glu Ser Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 100 105 110Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys 115 120 125Gln Leu Asp Pro Ala Asp Val Val Phe Phe Gln Arg Gln Arg Ser Asp 130 135 140Ser Arg Arg Leu Phe Ile Gly Trp Arg Arg Arg Gly Gln Gly Ser Ser145 150 155 160Ser Ala Ala Asn Thr Thr Ser Tyr Ser Ser Ser Met Thr Ala Pro Pro 165 170 175Tyr Ser Asn Tyr Ser Asn Arg Pro Ala His Ser Glu Tyr Ser His Tyr 180 185 190Gly Ala Ala Val Ala Thr Ala Thr Glu Thr His Phe Ile Pro Ser Ser 195 200 205Ser Ala Val Gly Ser Ser Arg Thr Val Arg Leu Phe Gly Val Asn Leu 210 215 220Glu Cys Gln Met Asp Glu Asp Glu Gly Asp Asp Ser Val Ala Thr Ala225 230 235 240Ala Ala Ala Glu Cys Pro Arg Gln Asp Ser Tyr Tyr Asp Gln Asn Met 245 250 255Tyr Asn Tyr Tyr Thr Pro His Ser Ser Ala Ser 260 265105804DNABrassica rapa 105atgtcagtca accattactc cacggaccac caccaggtcc accaccacca cactctcttc 60ttgcagaacc tccacaccac cgacacatcg gagccaacca caaccgccgc cacatcactc 120cgcgaagacc agaaagagta tctcttcgag aaatctctca caccaagcga cgttggcaaa 180ctcaaccgtc tcgttatacc aaaacagcac gcggagaagt acttccctct caacaccatc 240atctccaata atgctgagga gaaagggatg cttctaagct tcgaagacga gtcaggcaag 300tgctggaggt tcagatactc ttactggaac agcagtcaaa gctacgtgtt gactaaagga 360tggagcagat acgtcaaaga caaacagctc gacccagccg atgttgtttt cttccaacgt 420caacgttctg attcccggag actctttatt ggctggcgta gacgcggtca aggctcctcc 480tccgccgcga atacgacgtc gtattctagt tccatgactg ctccaccgta tagtaattac 540tctaatcgtc ctgctcactc agagtattcc cactatggcg ccgccgtagc aacagcgacg 600gagacgcact tcataccatc gtcttccgcc gtcgggagct cgaggacggt gaggcttttt 660ggtgtgaatt tggagtgtca aatggatgaa gacgaaggag atgattcggt tgccacggca 720gccgccgctg agtgtcctcg tcaggacagc tactacgacc aaaacatgta caattattac 780actcctcact cctcagcctc ataa 804106248PRTBrassica rapa 106Met Ser Ile Asn Gln Tyr Ser Ser Asp Phe Asn Tyr His Ser Leu Met1 5 10 15Trp Gln Gln Gln Gln His Arg His His His His Gln Asn Asp Val Ala 20 25 30Glu Glu Lys Glu Ala Leu Phe Glu Lys Pro Leu Thr Pro Ser Asp Val 35 40 45Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr 50 55 60Phe Pro Leu Ala Ala Ala Ala Ala Asp Ala Met Glu Lys Gly Leu Leu65 70 75 80Leu Cys Phe Glu Asp Glu Glu Gly Lys Pro Trp Arg Phe Arg Tyr Ser 85 90 95Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg 100 105 110Tyr Val Lys Glu Lys Gln Leu Asp Ala Gly Asp Val Ile Leu Phe His 115 120 125Arg His Arg Val Asp Gly Gly Arg Phe Phe Ile Gly Trp Arg Arg Arg 130 135 140Gly Asn Ser Ser Ser Ser Ser Asp Ser Tyr Arg His Leu Gln Ser Asn145 150 155 160Ala Ser Leu Gln Tyr Tyr Pro His Ala Gly Val Gln Ala Val Glu Ser 165 170 175Gln Arg Gly Asn Ser Lys Thr Leu Arg Leu Phe Gly Val Asn Met Glu 180 185 190Cys Gln Leu Asp Ser Asp Leu Pro Asp Pro Ser Thr Pro Asp Gly Ser 195 200 205Thr Ile Cys Pro Thr Ser His Asp Gln Phe His Leu Tyr Pro Gln Gln 210 215 220His Tyr Pro Pro Pro Tyr Tyr Met Asp Ile Ser Phe Thr Gly Asp Val225 230 235 240His Gln Thr Arg Ser Pro Gln Gly 245107747DNABrassica rapa 107atgtcaataa accaatactc aagcgatttc aactaccact ctctcatgtg gcaacaacag 60cagcaccgcc accaccacca tcaaaacgac gtcgcggagg aaaaagaagc tcttttcgag 120aaacccttaa ccccaagtga cgtcggaaaa ctcaaccgcc tcgtcatccc aaaacagcac 180gccgagagat acttccctct cgcagcagcc gccgcagacg cgatggagaa gggattactt 240ctctgcttcg aggacgagga aggtaagcca tggagattca gatactcgta ttggaacagt 300agccagagtt atgtcttgac caaaggatgg agcagatacg tcaaggagaa gcagctcgac 360gccggtgacg tcattctctt ccaccgccac cgtgttgacg gaggaagatt cttcattggc 420tggagaagac gcggcaactc ttcctcctct tccgactctt atcgccatct tcagtccaat 480gcctcgctcc aatattatcc tcatgcagga gttcaagcgg tggagagcca gagagggaat 540tcgaagacat taagactgtt cggagtgaac atggagtgtc agctagactc cgacttgccc 600gatccatcta caccagacgg ttccaccata tgtccgacca gtcacgacca gtttcatctc 660taccctcaac aacactatcc tcctccgtac tacatggaca taagtttcac aggagatgtg 720caccagacga gaagcccaca aggataa 747108245PRTBrassica rapa 108Met Ser Ile Asn Gln Tyr Ser Ser Glu Phe Tyr Tyr His Ser Leu Met1 5 10 15Trp Gln Gln Gln Gln Gln His His His Gln Asn Glu Val Val Glu Glu 20 25 30Lys Glu Ala Leu Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys 35 40 45Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro 50 55 60Leu Ala Ala Ala Ala Val Asp Ala Val Glu Lys Gly Leu Leu Leu Cys65 70 75 80Phe Glu Asp Glu Glu Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val 100 105 110Lys Glu Lys Gln Leu Asp Ala Gly Asp Val Val Leu Phe His Arg His 115 120 125Arg Ala Asp Gly Gly Arg Phe Phe Ile Gly Trp Arg Arg Arg Gly Asp 130 135 140Ser Ser Ser Ser Ser Asp Ser Tyr Arg Asn Leu Gln Ser Asn Ser Ser145 150 155 160Leu Gln Tyr Tyr Pro His Ala Gly Ala Gln Ala Val Glu Asn Gln Arg 165 170 175Gly Asn Ser Lys Thr Leu Arg Leu Phe Gly Val Asn Met Glu Cys Gln 180 185 190Ile Asp Ser Asp Trp Ser Glu Pro Ser Thr Pro Asp Gly Phe Thr Thr 195 200 205Cys Pro Thr Asn His Asp Gln Phe Pro Ile Tyr Pro Glu His Phe Pro 210 215 220Pro Pro Tyr Tyr Met Asp Val Ser Phe Thr Gly Asp Val His Gln Thr225 230 235 240Ser Ser Gln Gln Gly 245109738DNABrassica rapa 109atgtcaataa atcaatattc aagcgagttc tactaccatt ctctcatgtg gcaacaacag 60cagcaacacc accatcaaaa cgaagtcgtg gaggaaaaag aagctctttt cgagaaaccc 120ttaaccccaa gtgacgtcgg aaaactaaac cgcctagtca tccctaaaca gcacgccgag 180agatacttcc ctctcgccgc cgccgcggta gacgccgtgg agaagggatt actcctctgc 240ttcgaggacg aggaaggtaa gccatggaga ttcagatact cttattggaa tagtagccag 300agttacgtct tgaccaaagg atggagcaga tatgttaaag agaagcaact tgacgccggc 360gacgttgttc tctttcatcg ccaccgtgct gacggtggaa gattcttcat tggctggaga 420agacgcggcg actcttcctc ctcctccgac tcttatcgca atcttcaatc taattcctcg 480ctccaatatt atcctcatgc aggggctcaa gcggtggaga accagagagg taactccaag 540acattgagac tttttggagt gaacatggag tgccagatag actcagactg gtccgagcca 600tccacacctg acggttttac cacatgtcca accaatcacg accagtttcc tatctaccct 660gaacactttc ctcctccgta ctacatggac gtaagtttca caggagatgt gcaccagacg 720agtagccaac aaggatag 738110310PRTBrassica rapa 110Met Met Thr Asn Leu Ser Leu Ala Arg Glu Gly Glu Glu Glu Glu Glu1 5 10 15Glu Ala Gly Ala Lys Lys Pro Thr Glu Glu Val Glu Arg Glu His Met 20 25 30Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu 35 40 45Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Leu Asp Ser Ser 50 55 60Thr Asn Glu Lys Gly Leu Ile Leu Asn Phe Glu Asp Leu Thr Gly Lys65 70 75 80Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 85 90 95Met Thr Lys Gly Trp Ser Arg Phe Val Lys Asp Lys Lys Leu Asp Ala 100 105 110Gly Asp Ile Val Ser Phe Leu Arg Cys Val Gly Asp Thr Gly Arg Asp 115 120 125Ser Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Lys Val Pro Asp Tyr 130 135 140Thr Thr Ser Thr Ser His Phe Pro Ala Gly Ala Met Phe Pro Arg Phe145 150 155 160Tyr Ser Phe Gln Thr Ala Thr Thr Ser Thr Ser Tyr Asn Pro Tyr Asn 165 170 175His Gln Gln Pro Arg His His His Ser Gly Tyr Cys Tyr Pro Gln Ile 180 185 190Pro Arg Glu Phe Gly Tyr Gly Tyr Val Val Arg Ser Val Asp Gln Arg 195 200 205Ala Val Val Ala Asp Pro Leu Val Ile Glu Ser Val Pro Val Met Met 210 215 220His Gly Gly Ala Arg Val Asn Gln Ala Ala Val Gly Thr Ala Gly Lys225 230 235 240Arg Leu Arg Leu Phe Gly Val Asp Met Glu Cys Gly Glu Ser Gly Gly 245 250 255Thr Asn Ser Thr Glu Glu Glu Ser Ser Ser Ser Gly Gly Ser Leu Pro 260 265 270Arg Gly Gly Ala Ser Pro Ser Ser Ser Met Phe Gln Leu Arg Leu Gly 275 280 285Asn Ser Ser Glu Asp Asp His Leu Phe Lys Lys Gly Lys Ser Ser Leu 290 295 300Pro Phe Asn Leu Asp Gln305 310111933DNABrassica rapa 111atgatgacaa atttgtctct tgcaagagaa ggagaagaag aagaagaaga ggcaggagca 60aagaagccca cagaagaagt ggagagagag cacatgttcg acaaagtggt gactccaagt 120gacgtcggga aactaaaccg actcgtgatc ccaaagcaac acgcggagag atacttccct 180ttagattcat ccacaaacga gaagggtttg attctaaact tcgaagatct cacgggaaag 240tcatggaggt tccgttactc ttactggaac agcagtcaga gctatgtcat gactaaaggt 300tggagccgtt tcgttaaaga caagaagcta gacgctggag atattgtctc tttcctgaga 360tgtgtcggag acacaggaag ggacagccgc ttgtttatcg attggaggag acgacctaaa 420gtccctgact acacgacatc gacttctcac tttcctgccg gagctatgtt ccctaggttt 480tacagttttc agacagcaac tacttccaca agttacaatc cctataatca tcagcagcca 540cgtcatcatc acagtggtta ctgttatcct caaatcccga gagaatttgg atatgggtat 600gtcgttaggt cagtagatca gagggcggtg gtggctgatc cgttagtgat cgaatctgtg 660ccggtgatga tgcacggagg agctcgagtg aaccaggcgg ctgttggaac ggccgggaaa 720aggctgaggc tttttggagt cgatatggaa tgtggcgaga gtggaggaac aaacagtacg 780gaggaagaat cttcatcttc cggtgggagt ttgccacgtg gcggtgcttc tccgtcttcc 840tctatgtttc agctgaggct tggaaacagc agtgaagatg atcacttatt taagaaagga 900aagtcttcat tgccttttaa tttggatcaa taa 933112293PRTBrassica rapa 112Met Met Thr Asn Leu Ser Leu Ala Arg Glu Gly Glu Ala Gln Val Lys1 5 10 15Lys Pro Ile Glu Glu Val Glu Arg Glu His Met Phe Asp Lys Val Val 20 25 30Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln 35 40 45His Ala Glu Arg Tyr Phe Pro Leu Asp Ser Ser Ser Asn Glu Lys Gly 50 55 60Leu Leu Leu Asn Phe Glu Asp Leu Thr Gly Lys Ser Trp Arg Phe Arg65 70 75 80Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp 85 90 95Ser Arg Phe Val Lys Asp Lys Lys Leu Asp Ala Gly Asp Ile Val Ser 100 105 110Phe Gln Arg Cys Val Gly Asp Ser Arg Leu Phe Ile Asp Trp Arg Arg 115 120 125Arg Pro Lys Val Pro Asp Tyr Pro Thr Ser Thr Ala His Phe Ala Ala 130 135 140Gly Ala Met Phe Pro Arg Phe Tyr Ser Phe Pro Thr Ala Thr Thr Ser145 150 155 160Thr Cys Tyr Asp Leu Tyr Asn His Gln Pro Pro Arg His His His Ile 165 170 175Gly Tyr Gly Tyr Pro Gln Ile Pro Arg Glu Phe Gly Tyr Gly Tyr Phe 180 185 190Val Arg Ser Val Asp Gln Arg Ala Val Val Ala Asp Pro Leu Val Ile 195 200 205Glu Ser Val Pro Val Met Met Arg Gly Gly Ala Arg Val Ser Gln Glu 210 215 220Val Val Gly Thr Ala Gly Lys Arg Leu Arg Leu Phe Gly Val Asp Met225 230 235 240Glu Glu Glu Ser Ser Ser Ser Gly Gly Ser Leu Pro Arg Ala Gly Gly 245 250 255Gly Gly Ala Ser Ser Ser Ser Ser Leu Phe Gln Leu Arg Leu Gly Ser 260 265 270Ser Cys Glu Asp Asp His Phe Ser Lys Lys Gly Lys Ser Ser Leu Pro 275 280 285Phe Asp Leu Asp Gln 290113882DNABrassica rapa 113atgatgacca acttgtctct tgcaagggaa ggagaagcac aagtaaagaa gcccatagaa 60gaagttgaga gagagcacat gttcgacaaa gtggtgactc caagcgacgt agggaaacta 120aacagactcg tgatcccaaa gcaacacgca gagagatact tccctctaga ttcatcctca 180aacgagaaag gtttgcttct aaactttgaa gatctaacag gaaagtcatg gaggttccgt 240tactcttact ggaacagtag ccagagctat gtcatgacta aaggttggag tcgtttcgtt 300aaagacaaga agcttgacgc cggagatatt gtctctttcc agagatgtgt cggagacagc 360cgcttgttta tcgattggag gagacgacct aaagtccctg actatccgac atcgactgct 420cactttgctg caggagctat gttccctagg ttttacagtt ttccgacagc aactacttcg 480acatgttacg atctgtacaa tcatcagccg ccacgtcatc atcacattgg ttacggttat 540ccacagattc cgagagaatt tggatacggg tatttcgtta ggtcagtgga ccagagagcg 600gtggtggctg atccgttggt gatcgaatct gtgccggtga tgatgcgcgg aggagctcga 660gttagtcagg aggttgttgg aacggccggg aagaggctga ggctttttgg agtcgatatg 720gaggaagaat cttcatcttc cggtgggagt ttgccgcgtg ccggaggtgg cggtgcttct 780tcatcttcct ctttgtttca gctgagactt gggagcagct gtgaagatga tcacttctct 840aagaaaggaa agtcttcatt gccttttgat ttggatcaat aa 882114297PRTBrassica rapa 114Met Met Met Thr Asn Leu Ser Leu Ser Arg Glu Gly Glu Glu Glu Glu1 5 10 15Glu Glu Glu Gln Glu Glu Ala Lys Lys Pro Met Glu Glu Val Glu Arg 20 25

30Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu 35 40 45Asn Arg Leu Val Ile Pro Lys Gln Tyr Ala Glu Arg Tyr Phe Pro Leu 50 55 60Asp Ser Ser Thr Asn Glu Lys Gly Leu Leu Leu Asn Phe Glu Asp Leu65 70 75 80Ala Gly Lys Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln 85 90 95Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Asp Lys Lys 100 105 110Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Cys Val Gly Asp Ser 115 120 125Gly Arg Asp Ser Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Lys Val 130 135 140Pro Asp His Pro Thr Ser Ile Ala His Phe Ala Ala Gly Ser Met Phe145 150 155 160Pro Arg Phe Tyr Ser Phe Pro Thr Ala Thr Ser Tyr Asn Leu Tyr Asn 165 170 175Tyr Gln Gln Pro Arg His His His His Ser Gly Tyr Asn Tyr Pro Gln 180 185 190Ile Pro Arg Glu Phe Gly Tyr Gly Tyr Leu Val Asp Gln Arg Ala Val 195 200 205Val Ala Asp Pro Leu Val Ile Glu Ser Val Pro Val Met Met His Gly 210 215 220Gly Ala Gln Val Ser Gln Ala Val Val Gly Thr Ala Gly Lys Arg Leu225 230 235 240Arg Leu Phe Gly Val Asp Met Glu Glu Glu Ser Ser Ser Ser Gly Gly 245 250 255Ser Leu Pro Arg Gly Asp Ala Ser Pro Ser Ser Ser Leu Phe Gln Leu 260 265 270Arg Leu Gly Ser Ser Ser Glu Asp Asp His Phe Ser Lys Lys Gly Lys 275 280 285Ser Ser Leu Pro Phe Asp Leu Asp Gln 290 295115894DNABrassica rapa 115atgatgatga caaacttgtc tctttcaaga gaaggagaag aggaggaaga agaagaacaa 60gaagaggcca agaagcccat ggaagaagta gagagagagc acatgttcga caaagtggtg 120actccaagcg atgttggtaa actaaaccgg ctcgtgatcc caaagcaata cgcagagaga 180tacttccctt tagattcatc cacaaacgag aaaggtttgc ttctaaactt cgaagatctc 240gcaggaaagt catggaggtt ccgttactct tactggaaca gtagtcagag ctatgtcatg 300actaaaggtt ggagccgttt cgttaaagac aaaaagctag acgccggaga tattgtctct 360ttccagagat gtgtcggaga ttcaggaaga gacagccgct tgtttattga ttggaggaga 420agacctaaag ttcctgacca tccgacatcg attgctcact ttgctgccgg atctatgttt 480cctaggtttt acagttttcc gacagcaact agttacaatc tttacaacta tcagcagcca 540cgtcatcatc atcacagtgg ttataattat cctcaaattc cgagagaatt tggatacggg 600tacttggtgg atcaaagagc cgtggtggct gatccgttgg tgattgaatc tgtgccggtg 660atgatgcacg gaggagctca agttagtcag gcggttgttg gaacggccgg gaagaggctg 720aggctttttg gagtcgatat ggaggaagaa tcttcatctt ccggtgggag tttgccacgt 780ggtgacgctt ctccgtcttc ctctttgttt cagctgagac ttggaagcag cagtgaagat 840gatcacttct ctaagaaagg aaagtcctca ttgccttttg atttggatca ataa 894116286PRTBrassica rapa 116Met Asn Gln Glu Glu Glu Asn Pro Val Glu Lys Ala Ser Ser Met Glu1 5 10 15Arg Glu His Met Phe Glu Lys Val Val Thr Pro Ser Asp Val Gly Lys 20 25 30Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro 35 40 45Leu Asp Asn Asn Ser Asp Ser Ser Lys Gly Leu Leu Leu Asn Phe Glu 50 55 60Asp Arg Thr Gly Asn Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser65 70 75 80Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Asp 85 90 95Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Asp Pro Gly 100 105 110Asn Lys Asp Lys Leu Phe Ile Asp Trp Arg Arg Arg Pro Lys Ile Pro 115 120 125Asp His His His Gln Phe Ala Gly Ala Met Phe Pro Arg Phe Tyr Ser 130 135 140Phe Ser His Pro Gln Asn Leu Tyr His Arg Tyr Gln Gln Asp Leu Gly145 150 155 160Ile Gly Tyr Tyr Val Ser Ser Met Glu Arg Asn Asp Pro Thr Ala Val 165 170 175Ile Glu Ser Val Pro Leu Ile Met Gln Arg Arg Ala Ala His Val Ala 180 185 190Ala Ile Pro Ser Ser Arg Gly Glu Lys Arg Leu Arg Leu Phe Gly Val 195 200 205Asp Met Glu Cys Gly Gly Gly Gly Gly Ser Val Asn Ser Thr Glu Glu 210 215 220Glu Ser Ser Ser Ser Gly Gly Gly Gly Gly Val Ser Met Ala Ser Val225 230 235 240Gly Ser Leu Leu Gln Leu Arg Leu Val Ser Ser Asp Asp Glu Ser Leu 245 250 255Val Ala Met Glu Ala Ala Ser Val Asp Glu Asp His His Leu Phe Thr 260 265 270Lys Lys Gly Lys Ser Ser Leu Ser Phe Asp Leu Asp Arg Lys 275 280 285117861DNABrassica rapa 117atgaatcaag aagaagagaa tcctgtggaa aaagcctctt caatggagag agagcacatg 60tttgaaaaag tagtaacacc aagcgacgta ggcaaactaa accgactcgt gatcccaaag 120caacacgcgg agagatactt ccctttagac aacaattctg acagcagcaa aggtttgctt 180ctaaacttcg aagaccgaac aggaaactca tggagattcc gttactctta ctggaacagt 240agccagagtt atgtcatgac aaaaggttgg agccgcttcg tcaaagacaa gaagcttgat 300gctggcgaca tcgtttcttt tcagagagat cctggtaata aagacaagct tttcattgat 360tggaggagac gaccaaagat tccagatcat catcatcaat tcgctggagc tatgttccct 420aggttttact ctttctctca tcctcagaac ctttatcatc gatatcaaca agatcttgga 480attgggtatt atgtgagttc aatggagaga aatgatccaa cggctgtaat tgaatctgtg 540ccgttgataa tgcaaaggag agcagcacac gtggctgcta taccttcatc aagaggagag 600aagaggttaa ggctgtttgg agtggacatg gagtgcggcg gcggcggagg aagtgtgaat 660agcacggagg aagagtcgtc gtcttccggt ggtggcggcg gcgtttctat ggctagtgtt 720ggttctcttc tccaattgag gctagtgagc agtgatgatg agtctttggt agcaatggaa 780gctgcaagtg tcgatgagga tcatcacttg tttacaaaga aaggaaagtc ttctttgtct 840ttcgatttgg atagaaaatg a 861118292PRTBrassica rapa 118Met Asn Gln Glu Asn Lys Lys Pro Leu Glu Glu Ala Ser Thr Ser Met1 5 10 15Glu Arg Glu Asn Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly 20 25 30Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 35 40 45Pro Leu Asp Asn Ser Ser Thr Asn Asn Lys Gly Leu Leu Leu Asp Phe 50 55 60Glu Asp Arg Thr Gly Ser Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn65 70 75 80Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys 85 90 95Asp Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Asp Pro 100 105 110Cys Asn Lys Asp Lys Leu Tyr Ile Asp Trp Arg Arg Arg Pro Lys Ile 115 120 125Pro Asp His His Gln Phe Ala Gly Ala Met Phe Pro Arg Phe Tyr Ser 130 135 140Phe Pro His Pro Gln Met Pro Thr Ser Phe Glu Ser Ser His Asn Leu145 150 155 160Tyr His His Arg Phe Gln Arg Asp Leu Gly Ile Gly Tyr Tyr Pro Thr 165 170 175Ala Val Ile Glu Ser Val Pro Val Ile Met Gln Arg Arg Glu Ala Gln 180 185 190Val Ala Asn Met Ala Ser Ser Arg Gly Glu Lys Arg Leu Arg Leu Phe 195 200 205Gly Val Asp Val Glu Cys Gly Gly Gly Gly Gly Gly Ser Val Asn Ser 210 215 220Thr Glu Glu Glu Ser Ser Ser Ser Gly Gly Ser Met Ser Arg Gly Gly225 230 235 240Val Ser Met Ala Gly Val Gly Ser Leu Leu Gln Leu Arg Leu Val Ser 245 250 255Ser Asp Asp Glu Ser Leu Val Ala Met Glu Gly Ala Thr Val Asp Glu 260 265 270Asp His His Leu Phe Thr Thr Lys Lys Gly Lys Ser Ser Leu Ser Phe 275 280 285Asp Leu Asp Ile 290119879DNABrassica rapa 119atgaatcaag aaaacaagaa gcctttggaa gaagcttcga cttcaatgga gagagagaac 60atgttcgaca aagtagtaac accaagcgac gtagggaaac taaaccgact cgtgatccca 120aagcaacacg cagagagata cttcccttta gacaactcct caacaaacaa caaagggttg 180cttctagact tcgaagaccg tacaggaagc tcatggagat tccgttactc ttactggaac 240agtagccaaa gttatgtcat gacaaaaggt tggagccgtt ttgtcaaaga caagaagctt 300gatgctggtg acatcgtgtc ttttcaaaga gatccctgta ataaagacaa gctttacata 360gattggagga gacgaccaaa gattccagat catcatcagt tcgccggagc tatgttccct 420aggttttact ctttccctca ccctcagatg ccgacaagtt ttgaaagtag tcacaacctt 480tatcatcatc ggtttcaacg agatcttgga attgggtatt atccaacggc tgtgattgaa 540tctgtgccgg tgataatgca aaggagagaa gcacaagtgg ctaatatggc ttcatcaaga 600ggagagaaga ggttaaggct gtttggagtg gacgtggagt gcggcggcgg aggaggagga 660agtgtgaata gcacggagga agagtcgtcg tcttccggtg gtagtatgtc acgtggcggc 720gtttctatgg ctggtgttgg ttctctcctt cagttgaggt tagtgagcag tgatgatgag 780tctttagtag cgatggaagg tgctactgtc gatgaggatc atcacttgtt tacaactaag 840aaaggaaagt cttctttgtc tttcgatttg gatatatga 879120320PRTBrassica rapa 120Met Glu Arg Lys Ser Asn Asp Leu Glu Arg Ser Glu Asn Ile Asp Ser1 5 10 15Gln Asn Lys Lys Met Asn Leu Glu Glu Glu Arg Pro Val Gln Glu Ala 20 25 30Ser Ser Met Glu Arg Glu His Met Phe Asp Lys Val Val Thr Pro Ser 35 40 45Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu 50 55 60Arg Tyr Phe Pro Leu Asp Asn Asn Ser Ser Asp Asn Asn Lys Gly Leu65 70 75 80Leu Leu Asn Phe Glu Asp Arg Ile Gly Ile Leu Trp Ser Phe Arg Tyr 85 90 95Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser 100 105 110Arg Phe Val Lys Asp Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe 115 120 125His Arg Gly Ser Cys Asn Lys Asp Lys Leu Phe Ile Asp Trp Lys Arg 130 135 140Arg Pro Lys Ile Pro Asp His Gln Val Val Gly Ala Met Phe Pro Arg145 150 155 160Phe Tyr Ser Tyr Pro Tyr Pro Gln Ile Gln Ala Ser Tyr Glu Arg His 165 170 175Asn Leu Tyr His Arg Tyr Gln Arg Asp Ile Gly Ile Gly Tyr Tyr Val 180 185 190Arg Ser Met Glu Arg Tyr Asp Pro Thr Ala Val Ile Glu Ser Val Pro 195 200 205Val Ile Met Gln Arg Arg Ala His Val Ala Thr Met Ala Ser Ser Arg 210 215 220Gly Glu Lys Arg Leu Arg Leu Phe Gly Val Asp Met Glu Cys Val Arg225 230 235 240Gly Gly Arg Gly Gly Gly Gly Ser Val Asn Ser Thr Glu Glu Glu Ser 245 250 255Ser Thr Ser Gly Gly Ser Ile Ser Arg Gly Gly Val Ser Met Ala Gly 260 265 270Val Gly Ser Pro Leu Gln Leu Arg Leu Val Ser Ser Asp Gly Asp Asp 275 280 285Gln Ser Leu Val Ala Arg Gly Ala Ala Arg Val Asp Glu Asp His His 290 295 300Leu Phe Thr Lys Lys Gly Lys Ser Ser Leu Ser Phe Asp Leu Asp Lys305 310 315 320121963DNABrassica rapa 121atggagagga agtccaatga tcttgagaga tctgagaata ttgattctca aaacaagaag 60atgaatctag aagaagagag gcctgtacaa gaagcttctt cgatggagag agagcacatg 120ttcgacaaag tagtaacacc aagcgacgtt gggaaactaa accggctggt gatcccaaag 180caacacgcag agcgatactt ccctttagac aataattcct cagacaacaa caaaggtttg 240cttctaaact tcgaagatcg aataggaatc ttatggagtt tccgttactc ctactggaac 300agtagccaaa gttatgtaat gactaaaggc tggagccgtt tcgtcaaaga caagaagctt 360gatgctggcg acatagtttc ttttcataga ggttcttgta ataaagacaa gcttttcatt 420gattggaaga gacgaccaaa gattcctgat caccaagtcg tcggagctat gttccctagg 480ttttactctt acccttatcc tcagatacag gctagttatg aacgtcacaa cctttatcat 540cgatatcaac gagatatagg aattgggtat tatgtgaggt caatggagag atatgatcca 600acggctgtaa ttgaatctgt gccggtgata atgcaaagga gagcacatgt ggctactatg 660gcttcatcaa gaggagagaa gaggttaagg ctttttggag tggatatgga gtgcgtcaga 720ggcggccgag gaggaggagg aagtgtgaat agcacggagg aagagtcttc gacttccggt 780ggtagtatct cacgtggcgg cgtttctatg gctggtgttg gctctccact ccagttgagg 840ttagtgagca gtgacggtga tgatcagtct ctagtagcta ggggagctgc tagggttgat 900gaggatcatc acttgtttac aaagaaagga aagtcttctt tgtctttcga tttggataaa 960tga 963122350PRTBrassica rapa 122Met Val Phe Ser Cys Ile Asp Glu Ser Ser Ser Thr Ser Glu Ser Phe1 5 10 15Ser Pro Ala Thr Ala Thr Ala Thr Ala Thr Ala Thr Lys Phe Ser Ala 20 25 30Pro Pro Leu Pro Pro Leu Arg Leu Asn Arg Met Arg Ser Gly Gly Ser 35 40 45Asn Val Val Leu Asp Ser Lys Asn Gly Val Asp Ile Asp Ser Arg Lys 50 55 60Leu Ser Ser Ser Lys Tyr Lys Gly Val Val Pro Gln Pro Asn Gly Arg65 70 75 80Trp Gly Ala Gln Ile Tyr Val Lys His Gln Arg Val Trp Leu Gly Thr 85 90 95Phe Cys Asp Glu Glu Glu Ala Ala His Ser Tyr Asp Ile Ala Ala Arg 100 105 110Lys Phe Arg Gly Arg Asp Ala Val Val Asn Phe Lys Thr Phe Leu Ala 115 120 125Ser Glu Asp Asp Asn Gly Glu Leu Cys Phe Leu Glu Ala His Ser Lys 130 135 140Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr Ala Asp Glu Leu145 150 155 160Ala Gln Ser Asn Lys Arg Ser Gly Ala Asn Thr Asn Thr Asn Thr Thr 165 170 175Gln Ser His Thr Val Ser Arg Thr Arg Glu Val Leu Phe Glu Lys Val 180 185 190Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys 195 200 205Gln His Ala Glu Lys Tyr Phe Pro Leu Pro Ser Leu Ser Val Thr Lys 210 215 220Gly Val Leu Ile Asn Phe Glu Asp Val Thr Gly Lys Val Trp Arg Phe225 230 235 240Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly 245 250 255Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Arg Ala Gly Asp Val Val 260 265 270Thr Phe Glu Arg Ser Thr Gly Ser Asp Arg Gln Leu Tyr Ile Asp Trp 275 280 285Lys Ile Arg Ser Gly Pro Ser Lys Asn Pro Val Gln Val Val Val Arg 290 295 300Leu Phe Gly Val Asp Ile Phe Asn Val Thr Ser Ala Lys Pro Ser Asn305 310 315 320Val Val Asp Ala Cys Gly Gly Lys Arg Ser Arg Asp Val Asp Met Phe 325 330 335Ala Leu Arg Cys Ser Lys Lys His Ala Ile Ile Asn Ala Leu 340 345 3501231053DNABrassica rapa 123atggtattca gttgcataga cgagagctct tccacttcag aatctttttc acccgcaacc 60gcaaccgcaa ccgcaaccgc cacaaagttc tctgctcctc cgcttccacc gttacgcctc 120aaccggatga gaagcggtgg aagcaacgtc gtgttggatt caaagaatgg cgtagatatt 180gattcacgga agctatcgtc gtcaaagtac aaaggcgtgg ttcctcagcc caacggaaga 240tggggagctc agatttacgt gaagcaccag cgagtttggc tgggcacttt ctgcgatgaa 300gaggaagctg ctcactccta cgacatagcc gcccgtaaat tccgtggccg tgacgccgtt 360gtcaacttca aaaccttcct cgcctcagag gacgacaacg gcgagttatg tttccttgaa 420gctcactcca aggccgagat cgtcgacatg ttgaggaaac acacttacgc tgacgagctt 480gcgcagagca ataaacgcag cggagcgaat acgaatacga atacgactca aagccacacc 540gtttcgagaa cacgtgaagt gcttttcgag aaggttgtca cgcctagcga cgttggtaag 600ctaaaccgcc tcgtgatacc taaacagcac gcggagaaat attttccgtt accgtcactg 660tcggtgacta aaggcgttct gatcaacttc gaagacgtga cgggtaaggt gtggcggttc 720cgttactcat actggaacag tagtcaaagt tacgtgttga ccaagggatg gagtcggttc 780gttaaggaga agaatctccg agccggtgat gtcgttactt tcgagagatc gaccggttca 840gaccggcagc tttatattga ttggaaaatc cggtctggtc cgagcaaaaa ccctgttcag 900gttgtggtta ggcttttcgg agttgacatc ttcaacgtga caagcgcgaa gccgagcaac 960gttgtagacg cgtgcggtgg aaagagatct cgggatgttg atatgtttgc gctacggtgt 1020tccaaaaaac acgctataat caatgctttg tga 1053124540PRTZea mays 124Met Ala Ala Ser Pro Ser Ser Pro Leu Thr Ala Pro Pro Glu Pro Val1 5 10 15Thr Pro Pro Ser Pro Trp Thr Ile Thr Asp Gly Ala Ile Ser Gly Thr 20 25 30Leu Pro Ala Ala Glu Ala Phe Ala Val His Tyr Pro Gly Tyr Pro Ser 35 40 45Ser Pro Ala Arg Ala Ala Arg Thr Leu Gly Gly Leu Pro Gly Leu Ala 50 55 60Lys Val Arg Ser Ser Asp Pro Gly Ala Arg Leu Glu Leu Arg Phe Arg65 70 75 80Pro Glu Asp Pro Tyr Cys His Pro Ala Phe Gly Gln Ser Arg Ala Ser 85 90 95Thr Gly Leu Leu Leu Arg Leu Ser Lys Arg Lys Gly Ala Ala Ala Pro 100 105 110Cys Ala His Val Val Ala Arg Val Arg Thr Ala Tyr Tyr Phe Glu Gly 115 120 125Met Ala Asp Phe Gln His Val Val Pro Val His Ala Ala Gln Thr Arg 130

135 140Lys Arg Lys His Ser Asp Ser Gln Asn Asp Asn Glu Asn Phe Gly Ser145 150 155 160Asp Lys Thr Gly His Asp Glu Ala Asp Gly Asp Val Met Met Leu Val 165 170 175Pro Pro Leu Phe Ser Val Lys Asp Arg Pro Thr Lys Ile Ala Leu Val 180 185 190Pro Ser Ser Asn Ala Ile Ser Lys Thr Met His Arg Gly Val Val Gln 195 200 205Glu Arg Trp Glu Met Asn Val Gly Pro Thr Leu Ala Leu Pro Phe Asn 210 215 220Thr Gln Val Val Pro Glu Lys Ile Asn Trp Glu Asp His Ile Arg Lys225 230 235 240Asn Ser Val Glu Trp Gly Trp Gln Met Ala Val Cys Lys Leu Phe Asp 245 250 255Glu Arg Pro Val Trp Pro Arg Gln Ser Leu Tyr Glu Arg Phe Leu Asp 260 265 270Asp Asn Val His Val Ser Gln Asn Gln Phe Lys Arg Leu Leu Phe Arg 275 280 285Ala Gly Tyr Tyr Phe Ser Thr Gly Pro Phe Gly Lys Phe Trp Ile Arg 290 295 300Arg Gly Tyr Asp Pro Arg Lys Asp Ser Glu Ser Gln Ile Tyr Gln Arg305 310 315 320Ile Asp Phe Arg Met Pro Pro Glu Leu Arg Tyr Leu Leu Arg Leu Lys 325 330 335Asn Ser Glu Ser Arg Lys Trp Ala Asp Met Cys Lys Leu Glu Thr Met 340 345 350Pro Ser Gln Ser Phe Ile Tyr Leu Gln Leu Tyr Glu Leu Lys Asp Asp 355 360 365Phe Ile Gln Ala Glu Ile Arg Lys Pro Ser Tyr Gln Ser Val Cys Ser 370 375 380Arg Ser Thr Gly Trp Phe Ser Lys Pro Met Ile Lys Thr Leu Arg Leu385 390 395 400Gln Val Ser Ile Arg Leu Leu Ser Leu Leu His Asn Glu Glu Ala Lys 405 410 415Asn Leu Leu Arg Asn Ala His Glu Leu Ile Glu Arg Ser Lys Lys Gln 420 425 430Glu Ala Leu Ser Arg Ser Glu Leu Ser Ile Glu Tyr Asn Asp Ala Asp 435 440 445Gln Val Ser Ala Ala His Thr Gly Thr Glu Asp Gln Val Gly Pro Asn 450 455 460Asn Ser Asp Ser Glu Asp Val Asp Asp Glu Glu Glu Glu Glu Glu Leu465 470 475 480Glu Gly Tyr Asp Ser Pro Pro Met Ala Asp Asp Ile His Glu Phe Thr 485 490 495Leu Gly Asp Ser Tyr Ala Phe Gly Glu Gly Phe Ser Asn Gly Tyr Leu 500 505 510Glu Glu Val Leu Arg Ser Leu Pro Leu Gln Glu Asp Gly Gln Lys Lys 515 520 525Leu Cys Asp Ala Pro Ile Asn Ala Asp Ala Ser Asp 530 535 5401251674DNAZea mays 125atggccgcct cgccctcttc acccttgaca gcgccgccag agccggtgac cccgccgtcc 60ccatggacca tcacagacgg agccatctct ggcacgctcc cagcagccga ggccttcgca 120gtgcactacc cgggctaccc ctcctctccc gcccgcgccg cccgcaccct cggcggtctc 180cccggcctcg ccaaggtccg gagttccgat cccggcgccc gcctcgagct ccgcttccgc 240cccgaggacc cctactgcca tccagccttt ggccagtccc gcgcctccac tggccttctg 300ctgcgcctct ccaagcgcaa aggagctgcg gcaccttgtg cccatgtggt cgctcgtgtc 360cggactgctt actacttcga aggtatggca gattttcaac atgttgttcc agtgcatgct 420gcacaaacaa gaaaaagaaa acactcagat tctcaaaatg ataatgagaa ttttggtagt 480gataagacag gacatgatga agcagatgga gatgtcatga tgttggtacc ccctctcttt 540tcagtgaagg ataggccaac aaagatagcg cttgtaccat cgtccaatgc catatctaaa 600accatgcaca ggggagttgt acaagaacgg tgggagatga atgttggacc aactctggcg 660cttccgttca acactcaagt tgtcccggag aagattaatt gggaagacca cattagaaag 720aattctgtag aatggggttg gcaaatggct gtttgcaaat tgtttgatga gcgccctgtg 780tggccaaggc aatcacttta tgagcggttc cttgatgata atgtgcatgt ctctcaaaac 840caattcaaaa ggcttctgtt tagagctgga tactacttct ctactggacc ctttggaaaa 900ttttggatca gaagaggata tgaccctcgt aaagactctg agtcacaaat atatcagaga 960attgattttc gcatgcctcc cgagctacga tatcttctaa ggctgaagaa ttctgagtct 1020cgaaagtggg cagatatgtg caagcttgaa acaatgccat cacagagttt catctacctg 1080caattatatg aactgaagga tgattttatt caagcagaaa ttcgaaaacc ttcttatcaa 1140tcagtttgtt cacgttctac aggatggttt tctaagccaa tgatcaaaac cctgaggttg 1200caagtgagca taaggctcct ctctttattg cataatgaag aggctaaaaa cttgttgagg 1260aatgcccatg agcttattga aaggtccaag aagcaggaag ccctttcgag atctgagctg 1320tcaatagaat ataatgatgc tgatcaagtt tctgccgcac atactggaac tgaggatcaa 1380gtcggcccta acaactctga tagtgaagat gtggatgatg aagaagagga agaggaattg 1440gagggttatg attctccacc tatggcagat gatattcatg agttcacctt aggtgattcc 1500tatgcatttg gtgaaggctt ctcgaatgga tacctcgaag aagtactgcg cagcttgcca 1560ttgcaggaag acggccaaaa gaaattatgt gatgctccta tcaacgctga tgcaagtgat 1620ggagagtttg aaatttacga acagcccagt gatgatgaag attctgatgg ctag 1674126409PRTZea mays 126Met Glu Phe Ala Ser Ser Ser Ser Arg Phe Ser Arg Glu Glu Asp Glu1 5 10 15Glu Glu Glu Gln Glu Glu Glu Glu Glu Glu Glu Glu Ala Ser Pro Arg 20 25 30Glu Ile Pro Phe Met Thr Ala Ala Ala Thr Ala Asp Thr Gly Ala Ala 35 40 45Ala Ser Ser Ser Ser Pro Ser Ala Ala Ala Ser Ser Gly Pro Ala Ala 50 55 60Ala Pro Arg Ser Ser Asp Gly Ala Gly Ala Ser Gly Ser Gly Gly Gly65 70 75 80Gly Ser Asp Asp Val Gln Val Ile Glu Lys Glu His Met Phe Asp Lys 85 90 95Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 100 105 110Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala Ala Asn Glu 115 120 125Lys Gly Gln Leu Leu Ser Phe Glu Asp Arg Ala Gly Lys Leu Trp Arg 130 135 140Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys145 150 155 160Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr 165 170 175Val Ser Phe Cys Arg Gly Ala Gly Asp Thr Ala Arg Asp Arg Leu Phe 180 185 190Ile Asp Trp Lys Arg Arg Ala Asp Ser Arg Asp Pro His Arg Met Pro 195 200 205Arg Leu Pro Leu Pro Met Ala Pro Val Ala Ser Pro Tyr Gly Pro Trp 210 215 220Gly Gly Gly Gly Gly Gly Gly Ala Gly Gly Phe Phe Met Pro Pro Ala225 230 235 240Pro Pro Ala Thr Leu Tyr Glu His His Arg Phe Arg Gln Ala Leu Asp 245 250 255Phe Arg Asn Ile Asn Ala Ala Ala Ala Pro Ala Arg Gln Leu Leu Phe 260 265 270Phe Gly Ser Ala Gly Met Pro Pro Arg Ala Ser Met Pro Gln Gln Gln 275 280 285Gln Pro Pro Pro Pro Pro His Pro Pro Leu His Ser Ile Met Leu Val 290 295 300Gln Pro Ser Pro Ala Pro Pro Thr Ala Ser Val Pro Met Leu Leu Asp305 310 315 320Ser Val Pro Leu Val Asn Ser Pro Thr Ala Ala Ser Lys Arg Val Arg 325 330 335Leu Phe Gly Val Asn Leu Asp Asn Pro Gln Pro Gly Thr Ser Ala Glu 340 345 350Ser Ser Gln Asp Ala Asn Ala Leu Ser Leu Arg Thr Pro Gly Trp Gln 355 360 365Arg Pro Gly Pro Leu Arg Phe Phe Glu Ser Pro Gln Arg Gly Ala Glu 370 375 380Ser Ser Ala Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser Lys Arg Glu385 390 395 400Ala His Ser Ser Leu Asp Leu Asp Leu 405127259PRTZea mays 127Met Glu Phe Thr Thr Pro Pro Pro Ala Thr Arg Ser Gly Gly Gly Glu1 5 10 15Glu Arg Ala Ala Ala Glu His Asn Gln His His Gln Gln Gln His Ala 20 25 30Thr Val Glu Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp 35 40 45Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys 50 55 60Tyr Phe Pro Leu Asp Ala Ala Ala Asn Glu Lys Gly Leu Leu Leu Ser65 70 75 80Phe Glu Asp Arg Thr Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val 100 105 110Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr Val Ser Phe Gly Arg Gly 115 120 125Ile Ser Glu Ala Ala Arg Asp Arg Leu Phe Ile Asp Trp Arg Cys Arg 130 135 140Pro Asp Pro Pro Val Val His His Gln Tyr His His Arg Leu Pro Leu145 150 155 160Pro Ser Ala Val Val Pro Tyr Ala Pro Trp Ala Ala His Ala His His 165 170 175His His Tyr Pro Ala Asp Gly His Thr Glu Pro Val Thr Pro Cys Leu 180 185 190Cys Ala Thr Leu Val Ala Thr Glu Met Arg Ala Ser Ser Ser Gln Leu 195 200 205Ser Leu Thr Arg Ser Asn Leu Ser Arg Pro Pro Gln Pro Arg Ile Ala 210 215 220Arg Val Asp Gly Ala Gln Pro Arg Pro Ser Ser Ser Pro Arg Gln Pro225 230 235 240Gln Ser Leu Trp Cys Arg Ser Cys Gln Pro Gln Pro Arg Arg Thr Ala 245 250 255Asp Val Pro128780DNAZea mays 128atggagttca ccactccccc gcccgcgacc cggtcgggcg gcggagagga gagggcggct 60gctgagcaca accagcacca ccagcagcag catgcgacgg tggagaagga gcacatgttc 120gacaaggtgg tgacgccgag cgacgtcggg aagctgaacc ggctggtgat cccgaagcag 180cacgcggaga agtacttccc gctggacgcg gcggcgaacg agaagggcct cctgctcagc 240ttcgaggacc gcacggggaa gccctggcgc ttccgctact cctactggaa cagtagccag 300agctacgtga tgaccaaggg ctggagccgc ttcgtcaagg agaagcgcct cgacgccggg 360gacacagtct ccttcggccg cggcatcagc gaggcggcgc gcgacaggct tttcatcgac 420tggcggtgcc gacccgaccc gcccgtcgtg caccaccagt accaccaccg cctccctctc 480ccctccgccg tcgtccccta cgcgccgtgg gcggcgcacg cgcaccacca ccactaccca 540gcagatgggc acacggaacc agtaacacct tgcctgtgcg ccacactcgt tgccactgaa 600atgagagcat catcttcgca actgtcactc acacgctcca acctctccag gccgccacaa 660cctagaatag ccagagtcga tggcgcccag ccacggccgt cgtcgtcacc acgccagcca 720cagtcgttgt ggtgccggtc gtgccaaccg caaccacggc gaacggccga cgttccttga 780129327PRTZea mays 129Met Glu Phe Thr Ala Pro Pro Pro Ala Thr Arg Ser Gly Gly Gly Glu1 5 10 15Glu Arg Ala Ala Ala Glu His His Gln Gln Gln Gln Gln Ala Thr Val 20 25 30Glu Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly 35 40 45Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 50 55 60Pro Leu Asp Ala Ala Ala Asn Asp Lys Gly Leu Leu Leu Ser Phe Glu65 70 75 80Asp Arg Ala Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser 85 90 95Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu 100 105 110Lys Arg Leu Asp Ala Gly Asp Thr Val Ser Phe Gly Arg Gly Val Gly 115 120 125Glu Ala Ala Arg Gly Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Asp 130 135 140Pro Pro Val Val His His Gln Tyr His His His Arg Leu Pro Leu Pro145 150 155 160Ser Ala Val Val Pro Tyr Ala Pro Trp Ala Ala Ala Ala His Ala His 165 170 175His His His Tyr Pro Ala Ala Gly Val Gly Ala Ala Arg Thr Thr Thr 180 185 190Thr Thr Thr Thr Thr Val Leu His His Leu Pro Pro Ser Pro Ser Pro 195 200 205Leu Tyr Leu Asp Thr Arg Arg Arg His Val Gly Tyr Asp Ala Tyr Gly 210 215 220Ala Gly Thr Arg Gln Leu Leu Phe Tyr Arg Pro His Gln Gln Pro Ser225 230 235 240Thr Thr Val Met Leu Asp Ser Val Pro Val Arg Leu Pro Pro Thr Pro 245 250 255Gly Gln His Ala Glu Pro Pro Pro Pro Ala Val Ala Ser Ser Ala Ser 260 265 270Lys Arg Val Arg Leu Phe Gly Val Asn Leu Asp Cys Ala Ala Ala Ala 275 280 285Gly Ser Glu Glu Glu Asn Val Gly Gly Trp Arg Thr Ser Ala Pro Pro 290 295 300Thr Gln Gln Ala Ser Ser Ser Ser Ser Tyr Ser Ser Gly Lys Ala Arg305 310 315 320Cys Ser Leu Asn Leu Asp Leu 325130984DNAZea mays 130atggagttca ccgctccccc gcccgcgacc cggtcgggcg gcggcgagga gagggcggct 60gctgagcacc accagcagca gcagcaggcg acggtggaga aggagcacat gttcgacaag 120gtggtgacgc cgagcgacgt cgggaagctg aaccggctgg tgatcccgaa gcagcacgcg 180gagaggtact tcccgctgga cgcggcggcg aacgacaagg gcctgctgct cagcttcgag 240gaccgcgcgg ggaagccctg gcgcttccgc tactcctact ggaacagcag ccagagctac 300gtgatgacca agggctggag ccgcttcgtc aaggagaagc gcctcgacgc cggggacacc 360gtctccttcg gccgcggcgt cggcgaggcg gcgcgcggca ggctcttcat cgactggcgg 420cgccgacccg acccgcccgt cgtgcaccac cagtaccacc accaccgcct ccctctcccc 480tccgccgtcg tcccctacgc gccgtgggcg gcggcggcgc acgcgcacca ccaccactac 540ccagcagctg gggtcggtgc cgccaggacg acgacgacga cgacgacgac ggtgctccac 600cacctgccgc cctcgccctc cccgctctac cttgacaccc gccgccgcca cgtcggctac 660gacgcctacg gggccggcac caggcaactt ctcttctaca ggccgcacca gcagccctcc 720acgacggtga tgctggactc cgtgccggta cggttaccgc caacgccagg gcagcacgcc 780gagccgccgc cccccgccgt ggcgtcgtca gcctcgaagc gggtgcgcct gttcggggtg 840aacctcgact gcgccgccgc cgccggctca gaggaggaga acgtcggcgg gtggaggact 900agtgcgccgc cgacgcagca ggcgtcctcc tcctcatcct actcttccgg gaaagcgagg 960tgctccttga accttgactt gtga 984131422PRTZea mays 131Met Asp Gln Phe Ala Ala Ser Gly Arg Phe Ser Arg Glu Glu Glu Ala1 5 10 15Asp Glu Glu Gln Glu Asp Ala Ser Asn Ser Met Arg Glu Ile Ser Phe 20 25 30Met Pro Pro Ala Ala Ala Ser Ser Ser Ser Ala Ala Ala Ser Ala Ser 35 40 45Ala Ser Ala Ser Thr Ser Ala Ser Ala Cys Ala Ser Gly Ser Ser Ser 50 55 60Ala Pro Phe Arg Ser Ala Ser Ala Ser Gly Asp Ala Ala Gly Ala Ser65 70 75 80Gly Ser Gly Gly Pro Ala Asp Ala Asp Ala Glu Ala Glu Ala Val Glu 85 90 95Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys 100 105 110Leu Asn Arg Leu Val Ile Pro Lys Gln Tyr Ala Glu Lys Tyr Phe Pro 115 120 125Leu Asp Ala Ala Ala Asn Glu Lys Gly Leu Leu Leu Ser Phe Glu Asp 130 135 140Ser Ala Gly Lys His Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser145 150 155 160Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 165 170 175Arg Leu Val Ala Gly Asp Thr Val Ser Phe Ser Arg Ala Ala Ala Glu 180 185 190Asp Ala Arg His Arg Leu Phe Ile Asp Trp Lys Arg Arg Val Asp Thr 195 200 205Arg Gly Pro Leu Arg Phe Ser Gly Leu Ala Leu Pro Met Pro Leu Pro 210 215 220Ser Ser His Tyr Gly Gly Pro His His Tyr Ser Pro Trp Gly Phe Gly225 230 235 240Gly Gly Gly Gly Gly Gly Gly Gly Phe Phe Met Pro Pro Ser Pro Pro 245 250 255Ala Thr Leu Tyr Glu His Arg Leu Arg Gln Gly Leu Asp Phe Arg Ser 260 265 270Met Thr Thr Thr Tyr Pro Ala Pro Thr Val Gly Arg Gln Leu Leu Phe 275 280 285Phe Gly Ser Ala Arg Met Pro Pro His His Ala Pro Pro Pro Gln Pro 290 295 300Arg Pro Phe Ser Leu Pro Leu His His Tyr Thr Val Gln Pro Ser Ala305 310 315 320Ala Gly Val Thr Ala Ala Ser Arg Pro Val Leu Leu Asp Ser Val Pro 325 330 335Val Ile Glu Ser Pro Thr Thr Ala Ala Lys Arg Val Arg Leu Phe Gly 340 345 350Val Asn Leu Asp Asn Asn Pro Asp Gly Gly Gly Glu Ala Ser His Gln 355 360 365Gly Asp Ala Leu Ser Leu Gln Met Pro Gly Trp Gln Gln Arg Thr Pro 370 375 380Thr Leu Arg Leu Leu Glu Leu Pro Arg His Gly Gly Glu Ser Ser Ala385 390 395 400Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser Lys Arg Glu Ala Arg Ser 405 410 415Ala Leu Asp Leu Asp Leu 4201321269DNAZea mays 132atggaccagt tcgccgcgag cgggaggttc tctagagagg aggaggcgga cgaggagcag 60gaggatgcgt ccaattccat gcgcgagatc tccttcatgc cgccggctgc ggcctcgtca 120tcttcggcgg ctgcttccgc gtccgcgtcc gcctccacca gcgcatccgc gtgtgcatcg 180ggaagcagca gcgccccctt ccgctccgcc tccgcgtcgg gggatgccgc cggagcgtcg 240gggagcggcg gcccagcgga cgcggacgcg gaggcggagg cggtggagaa ggagcacatg 300ttcgacaagg tggtcacgcc gagcgacgtg gggaagctca accggctggt gatcccgaag 360cagtacgcgg agaagtactt cccgctggac gcggcggcca

acgagaaggg cctcctcctc 420agcttcgagg acagcgccgg caagcactgg cgcttccgct actcctactg gaacagcagc 480cagagctacg tcatgaccaa gggctggagc cgcttcgtca aggagaagcg cctcgtcgcc 540ggggacaccg tctccttctc ccgcgccgcc gccgaggacg cgcgccaccg cctcttcatc 600gactggaagc gccgggtcga cacccgcggc ccgcttcgtt tctccggcct cgcgctgccg 660atgccgctgc cgtcgtcgca ctacggcggg ccccaccact acagcccgtg gggcttcggc 720ggcggcggcg gcggcggcgg cggattcttc atgccgccct cgccgcccgc cacgctctac 780gagcaccgcc tcagacaggg cctcgacttc cgcagcatga cgacgaccta ccccgcgccg 840accgtgggga ggcagctcct gtttttcggc tcggccagga tgcctcctca tcacgcgccg 900ccgccccagc cgcgcccgtt ctcgctgccg ctgcatcact acacggtgca accgagcgcc 960gccggcgtca ccgccgcgtc acggccggtc cttcttgact cggtgccggt catcgagagc 1020ccgacgaccg ccgcgaagcg cgtgcggctg ttcggcgtca acctggacaa caacccagat 1080ggcggcggcg aggctagcca tcagggcgat gcattgtcat tgcagatgcc cgggtggcag 1140caaaggactc caactctaag gctactagaa ttgcctcgcc atggcgggga gtcctccgcg 1200gcgtcgtctc cgtcgtcgtc gtcttcctcc aagagggagg cgcgttcagc tttggatctc 1260gatctgtga 1269133894DNABrassica rapa 133atgatgatga caaacttgtc tctttcaaga gaaggagaag aggaggaaga agaagaacaa 60gaagaggcca agaagcccat ggaagaagta gagagagagc acatgttcga caaagtggtg 120actccaagcg atgttggtaa actaaaccgg ctcgtgatcc caaagcaata cgcagagaga 180tacttccctt tagattcatc cacaaacgag aaaggtttgc ttctaaactt cgaagatctc 240gcaggaaagt catggaggtt ccgttactct tactggaaca gtagtcagag ctatgtcatg 300actaaaggtt ggagccgttt cgttaaagac aaaaagctag acgccggaga tattgtctct 360ttccagagat gtgtcggaga ttcaggaaga gacagccgct tgtttattga ttggaggaga 420agacctaaag ttcctgacca tccgacatcg attgctcact ttgctgccgg atctatgttt 480cctaggtttt acagttttcc gacagcaact agttacaatc tttacaacta tcagcagcca 540cgtcatcatc atcacagtgg ttataattat cctcaaattc cgagagaatt tggatacggg 600tacttggtgg atcaaagagc cgtggtggct gatccgttgg tgattgaatc tgtgccggtg 660atgatgcacg gaggagctca agttagtcag gcggttgttg gaacggccgg gaagaggctg 720aggctttttg gagtcgatat ggaggaagaa tcttcatctt ccggtgggag tttgccacgt 780ggtgacgctt ctccgtcttc ctctttgttt cagctgagac ttggaagcag cagtgaagat 840gatcacttct ctaagaaagg aaagtcctca ttgccttttg atttggatca ataa 894134307PRTZea mays 134Met Ala Thr Asn His Leu Ser Gln Gly Gln His Gln His Pro Gln Ala1 5 10 15Trp Pro Trp Gly Val Ala Met Tyr Thr Asn Leu His Tyr His His Gln 20 25 30Gln His His His Tyr Glu Lys Glu His Leu Phe Glu Lys Pro Leu Thr 35 40 45Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His 50 55 60Ala Glu Arg Tyr Phe Pro Leu Ser Ser Ser Gly Ala Gly Asp Lys Gly65 70 75 80Leu Ile Leu Cys Phe Glu Asp Asp Asp Asp Asp Glu Ala Ala Ala Ala 85 90 95Asn Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Thr Ser Ser Gln Ser 100 105 110Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Glu Lys Gln Leu 115 120 125Asp Ala Gly Asp Val Val Arg Phe Gln Arg Met Arg Gly Phe Gly Met 130 135 140Pro Asp Arg Leu Phe Ile Ser His Ser Arg Arg Gly Glu Thr Thr Ala145 150 155 160Thr Ala Ala Thr Thr Val Pro Pro Ala Ala Ala Ala Val Arg Val Val 165 170 175Val Ala Pro Ala Gln Ser Ala Gly Ala Asp His Gln Gln Gln Gln Gln 180 185 190Pro Ser Pro Trp Ser Pro Met Cys Tyr Ser Thr Ser Gly Ser Tyr Ser 195 200 205Tyr Pro Thr Ser Ser Pro Ala Asn Ser Gln His Ala Tyr His Arg His 210 215 220Ser Ala Asp His Asp His Ser Asn Asn Met Gln His Ala Gly Glu Ser225 230 235 240Gln Ser Asp Arg Asp Asn Arg Ser Cys Ser Ala Ala Ser Ala Pro Pro 245 250 255Pro Pro Ser Arg Arg Leu Arg Leu Phe Gly Val Asn Leu Asp Cys Gly 260 265 270Pro Gly Pro Glu Pro Glu Thr Pro Thr Ala Met Tyr Gly Tyr Met His 275 280 285Gln Ser Pro Tyr Ala Tyr Asn Asn Trp Gly Ser Pro Tyr Gln His Asp 290 295 300Glu Glu Ile305135924DNAZea mays 135atggccacga accatctctc ccaagggcag caccagcacc cgcaggcctg gccctggggc 60gtggccatgt acaccaacct acactaccac caccagcagc accaccacta cgagaaggag 120cacctgttcg agaagccgct gacgccgagc gacgtgggca agctcaacag gctggtgatc 180cccaagcagc acgccgagag gtacttccct ctcagcagca gcggcgccgg cgacaaaggc 240ctcatcctgt gcttcgagga cgacgacgac gacgaggctg ccgccgccaa caagccgtgg 300cggttccgct actcgtactg gaccagcagc cagagctacg tgctcaccaa gggctggagc 360cgctacgtca aggagaagca gcttgacgcc ggcgacgtcg tgcgcttcca gaggatgcgt 420ggtttcggca tgcccgaccg cctgttcatc agccacagcc gccgcggcga gactactgct 480actgctgcaa caacagtgcc ccccgctgct gctgccgtgc gcgtagtagt ggcacctgca 540cagagcgctg gcgcagacca ccagcagcag cagcagccgt cgccttggag cccaatgtgc 600tacagcacat caggctcgta ctcgtacccc accagcagcc cagccaattc ccagcatgcc 660taccaccgcc actcagctga ccatgaccac agcaacaaca tgcaacatgc aggagaatct 720cagtccgaca gagacaacag gagctgcagt gcagcttcgg caccgccgcc accgtcgcgg 780cggctccggc tgttcggcgt aaacctcgac tgcggcccgg ggccggagcc ggagacacca 840acggcgatgt acggctacat gcaccaaagc ccctacgctt acaacaactg gggcagtcca 900taccagcatg acgaggagat ttaa 924136277PRTZea mays 136Met Glu Phe Thr Pro Ala His Ala His Ala Arg Val Val Glu Asp Ser1 5 10 15Glu Arg Pro Arg Gly Gly Val Ala Trp Val Glu Lys Glu His Met Phe 20 25 30Glu Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val 35 40 45Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Ala Leu Asp Ala Ser 50 55 60Ser Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Gly Lys Gly65 70 75 80Leu Val Leu Ser Phe Glu Asp Arg Ala Gly Lys Ala Trp Arg Phe Arg 85 90 95Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp 100 105 110Ser Arg Phe Val Lys Glu Lys Arg Leu Gly Ala Gly Asp Thr Val Leu 115 120 125Phe Ala Arg Gly Ala Gly Gly Ala Arg Gly Arg Phe Phe Ile Asp Phe 130 135 140Arg Arg Arg Arg Gln Asp Leu Ala Phe Leu Gln Pro Thr Leu Ala Ser145 150 155 160Ala Gln Arg Leu Leu Pro Leu Pro Ser Val Pro Ile Cys Pro Trp Gln 165 170 175Asp Tyr Gly Ala Ser Ala Pro Ala Pro Asn Arg His Val Leu Phe Leu 180 185 190Arg Pro Gln Val Pro Ala Ala Val Val Leu Lys Ser Val Pro Val His 195 200 205Val Ala Ala Ser Ala Val Glu Ala Thr Met Ser Lys Arg Val Arg Leu 210 215 220Phe Gly Val Asn Leu Asp Cys Pro Pro Asp Ala Glu Asp Ser Ala Thr225 230 235 240Val Pro Arg Gly Arg Ala Ala Ser Thr Thr Leu Leu Gln Leu Pro Ser 245 250 255Pro Ser Ser Ser Thr Ser Ser Ser Thr Ala Gly Lys Asp Val Cys Cys 260 265 270Leu Asp Leu Gly Leu 275137834DNAZea mays 137atggagttca cgcccgcgca tgcgcatgcc cgtgtcgttg aggattccga gaggcctcgc 60ggcggcgtgg cctgggtgga gaaggagcac atgttcgaga aggtggtcac cccgagcgac 120gtggggaagc tcaatcgcct ggtcatccca aagcagcacg cggagcgcta cttccccgcg 180ctggacgcct cgtccgccgc ggcggcggcg gcggcagcag ccgcgggagg cgggaagggg 240ctggtgctca gcttcgagga ccgggcgggg aaggcgtggc gcttccgcta ctcgtactgg 300aacagcagcc agagctacgt gatgaccaaa ggttggagcc gcttcgtgaa ggagaagcgc 360ctcggtgccg gggacacagt cttgttcgcg cgcggcgcgg gcggcgcgcg cggccgcttc 420ttcatcgatt tccgccgccg tcgccaggat ctcgcgttcc tgcagccgac gctggcgtct 480gcgcagcgac tcctgccgct gccgtcggtg cccatctgcc cgtggcagga ctacggcgcc 540tcggctccgg cgcccaaccg gcacgtgctg ttcctgcggc cgcaggtgcc ggccgccgta 600gtgctcaagt cggtccccgt gcacgttgct gcatccgcgg tggaggcgac catgtcgaag 660cgcgtccgcc tgttcggggt gaacctcgac tgcccgccgg acgccgaaga cagcgccaca 720gtcccccggg gccgggcggc gtcgacgacg cttctgcaac tgccctcgcc atcgtcgtca 780acatcctcct cgacggcagg gaaggacgtg tgctgtttgg atcttggact gtga 834138273PRTZea mays 138Met Glu Phe Arg Pro Ala His Ala Arg Val Phe Glu Asp Ser Glu Arg1 5 10 15Pro Arg Gly Gly Val Ala Trp Leu Glu Lys Glu His Met Phe Glu Lys 20 25 30Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 35 40 45Lys Gln His Ala Glu Arg Tyr Phe Pro Ala Leu Asp Ala Ser Ala Ala 50 55 60Ala Ala Ser Ala Ser Ala Ser Ala Gly Gly Gly Lys Ala Gly Leu Val65 70 75 80Leu Ser Phe Glu Asp Arg Ala Gly Lys Ala Trp Arg Phe Arg Tyr Ser 85 90 95Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg 100 105 110Phe Val Lys Glu Lys Arg Leu Gly Ala Gly Asp Thr Val Leu Phe Ala 115 120 125Arg Gly Ala Gly Ala Thr Arg Gly Arg Phe Phe Ile Asp Phe Arg Arg 130 135 140Arg Arg His Glu Leu Ala Phe Leu Gln Pro Pro Leu Ala Ser Ala Gln145 150 155 160Arg Leu Leu Pro Leu Pro Ser Val Pro Ile Cys Pro Trp Gln Gly Tyr 165 170 175Gly Ala Ser Ala Pro Ala Pro Ser Arg His Val Leu Phe Leu Arg Pro 180 185 190Gln Val Pro Ala Ala Val Val Leu Thr Ser Val Pro Val Arg Val Ala 195 200 205Ala Ser Ala Val Glu Glu Ala Thr Arg Ser Lys Arg Val Arg Leu Phe 210 215 220Gly Val Asn Leu Asp Cys Pro Pro Asp Ala Glu Asp Gly Ala Thr Ala225 230 235 240Thr Arg Thr Pro Ser Thr Leu Leu Gln Leu Pro Ser Pro Ser Ser Ser 245 250 255Thr Ser Ser Ser Thr Gly Gly Lys Asp Val Arg Ser Leu Asp Leu Gly 260 265 270Leu139822DNAZea mays 139atggagttca ggcccgcgca tgcccgtgtc ttcgaggatt ccgagaggcc tcgcggcggc 60gtggcgtggc tggagaagga gcacatgttc gagaaagtgg tcaccccgag cgacgtgggg 120aagctcaatc gcctggtcat cccgaagcag cacgccgagc gctacttccc cgcgctggac 180gcctcggccg ccgcggcgtc ggcatcggcg tcggcgggcg gcgggaaggc ggggctggtg 240ctcagcttcg aggaccgggc ggggaaggcg tggcgcttcc gctactcgta ctggaacagc 300agccagagct acgtgatgac caagggatgg agccgcttcg tgaaagagaa gcgcctcggt 360gccggggaca cggtattgtt cgcgcgcggc gcgggcgcca cgcgcggccg cttcttcatc 420gatttccgcc gccgccgcca cgagctcgcg ttcctgcagc cgccgctggc gtctgcgcag 480cgcctcctgc cgctcccgtc ggtgcccatc tgcccgtggc agggctacgg cgcctccgct 540ccggcgccaa gccggcacgt gctgttcctg cggccgcagg tgccggccgc cgtagtgctc 600acgtcggtgc ccgtgcgcgt cgccgcatcc gcggtggagg aggcgacgag gtcgaagcgc 660gtccgcctgt tcggggtgaa cctcgactgc ccgccggacg ccgaagacgg tgccacagcc 720acccggacgc cgtcgacgct tctgcagctg ccctcgccat cgtcgtcaac atcctcctcc 780acgggaggca aggatgtgcg ttctttggat cttggacttt ga 822140350PRTTriticum aestivum 140Met Gly Val Glu Ile Leu Ser Ser Met Val Glu His Ser Phe Gln Tyr1 5 10 15Ser Ser Gly Val Ser Thr Ala Thr Thr Glu Ser Gly Thr Ala Gly Thr 20 25 30Pro Pro Arg Pro Leu Ser Leu Pro Val Ala Ile Ala Asp Glu Ser Val 35 40 45Thr Ser Arg Ser Ala Ser Ser Arg Phe Lys Gly Val Val Pro Gln Pro 50 55 60Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Arg His Ala Arg Val Trp65 70 75 80Leu Gly Thr Phe Pro Asp Gln Asp Ser Ala Ala Arg Ala Tyr Asp Val 85 90 95Ala Ser Leu Arg Tyr Arg Gly Arg Asp Val Ala Phe Asn Phe Pro Cys 100 105 110Ala Ala Val Glu Gly Glu Leu Ala Phe Leu Ala Ala His Ser Lys Ala 115 120 125Glu Ile Val Asp Met Leu Arg Lys Gln Thr Tyr Ala Asp Glu Leu Arg 130 135 140Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Ala Arg Ala Gln Pro Thr145 150 155 160Pro Ser Trp Ala Arg Glu Pro Leu Phe Glu Lys Ala Val Thr Pro Ser 165 170 175Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln His Ala Glu 180 185 190Lys His Phe Pro Leu Lys Arg Thr Pro Glu Thr Pro Thr Thr Thr Gly 195 200 205Lys Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp Arg 210 215 220Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys225 230 235 240Gly Trp Ser Arg Phe Val Arg Glu Lys Gly Leu Gly Ala Gly Asp Ser 245 250 255Ile Leu Phe Ser Cys Ser Leu Tyr Glu Gln Glu Lys Gln Phe Phe Ile 260 265 270Asp Cys Lys Lys Asn Thr Ser Met Asn Gly Gly Lys Ser Ala Ser Pro 275 280 285Leu Pro Val Gly Val Thr Thr Lys Gly Glu Gln Val Arg Val Val Arg 290 295 300Leu Phe Gly Val Asp Ile Ser Gly Val Lys Arg Gly Arg Ala Ala Thr305 310 315 320Ala Thr Ala Glu Gln Gly Leu Gln Glu Leu Phe Lys Arg Gln Cys Val 325 330 335Ala Pro Gly Gln His Ser Pro Ala Leu Gly Ala Phe Ala Leu 340 345 3501411053DNATriticum aestivum 141atgggggtgg aaatcctgag ctccatggtg gagcactcct tccagtactc ttccggcgtg 60tccacggcca cgacggagtc aggcaccgcc ggaacaccgc cgaggccttt gagcctacct 120gtcgccatcg ccgacgagtc cgtgacctcg cggtcggcgt cgtctcggtt caagggcgtg 180gtgccgcagc caaacgggcg atggggcgcc cagatctacg agcgccacgc tcgcgtctgg 240ctcggcacgt tcccagacca ggactcggcg gcgcgcgcct acgacgtagc ctcgctcagg 300taccgcggcc gcgacgtcgc cttcaacttc ccgtgcgcgg ccgtggaggg ggagctcgcc 360ttcctggcgg cgcactccaa ggctgagata gtggacatgc tccggaagca gacctacgcc 420gatgaactcc gccagggcct gcggcgcggc cgtggcatgg gggcgcgcgc gcagccgacg 480ccgtcgtggg cgcgggagcc ccttttcgag aaggccgtga cccctagcga tgtcggcaag 540ctcaatcgcc tcgtagtgcc gaagcagcac gccgagaagc acttccccct gaagcgcacg 600ccggagacgc cgaccaccac cggcaagggc gtgctgctca acttcgagga cggcgagggg 660aaggtgtgga ggttccggta ctcgtactgg aacagcagcc agagctacgt gctcaccaaa 720ggctggagcc gcttcgtccg ggagaagggc ctaggtgccg gcgactccat cctattctcg 780tgctcgctgt acgaacagga gaagcagttc ttcatcgact gcaagaagaa cactagcatg 840aacggaggca aatcggcgtc gccgctgcca gtgggggtga ctaccaaagg agaacaagtt 900cgcgtcgtta ggctattcgg tgtcgacatc tcgggagtga agagggggcg agcggcgacg 960gcaacggcgg agcaaggcct gcaggagttg ttcaagaggc aatgcgtggc acccggccag 1020cactctcctg ccctaggtgc cttcgcctta tag 1053142320PRTTriticum aestivum 142Met Ala Ser Gly Lys Pro Thr Asn His Gly Met Glu Asp Asp Asn Asp1 5 10 15Met Glu Tyr Ser Ser Ala Glu Ser Gly Ala Glu Asp Ala Ala Glu Pro 20 25 30Ser Ser Ser Pro Val Leu Ala Pro Pro Arg Ala Ala Pro Ser Ser Arg 35 40 45Phe Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile 50 55 60Tyr Glu Lys His Ser Arg Val Trp Leu Gly Thr Phe Pro Asp Glu Asp65 70 75 80Ala Ala Val Arg Ala Tyr Asp Val Ala Ala Leu Arg Phe Arg Gly Pro 85 90 95Asp Ala Val Ile Asn His Gln Arg Pro Thr Ala Ala Glu Glu Ala Gly 100 105 110Ser Ser Ser Ser Arg Ser Glu Leu Asp Pro Glu Leu Gly Phe Leu Ala 115 120 125Asp His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Asp Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Arg Ala Gln145 150 155 160Pro Thr Pro Ala Trp Ala Arg Glu Leu Leu Phe Glu Lys Ala Val Thr 165 170 175Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln Gln 180 185 190Ala Glu Lys His Phe Pro Pro Thr Thr Ala Ala Ala Thr Gly Ser Asn 195 200 205Gly Lys Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp 210 215 220Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr225 230 235 240Lys Gly Trp Ser Arg Phe Val Lys Glu Thr Gly Leu Arg Ala Gly Asp 245 250 255Thr Val Ala Phe Tyr Arg Ser Ala Tyr Gly Asn Asp Thr Glu Asp Gln 260 265 270Leu Phe Ile Asp Tyr Lys Lys Met Asn Lys Asn Asp Asp Ala Ala Asp 275 280 285Ala Ala Ile Ser Asp Glu Asn Glu Thr Gly His Val Ala Val Lys Leu 290 295 300Phe Gly Val Asp Ile Ala Gly Gly Gly Met Ala Gly Ser Ser Gly Gly305 310 315 320143963DNATriticum aestivum 143atggcatctg

gcaagccgac aaaccacggg atggaggacg acaacgacat ggagtactcc 60tccgcggaat cgggggccga ggacgcggcg gagccgtcgt cgtcgccggt gctggcgccg 120ccccgggcgg ctccatcgtc gcggttcaag ggcgtcgtgc cgcagcccaa cgggcggtgg 180ggagcgcaga tctacgagaa gcactcgcgg gtgtggctcg gaacgttccc cgacgaggac 240gccgccgtgc gcgcctacga cgtggccgcg ctccgcttcc gcggcccgga cgccgtcatc 300aaccaccagc gaccgacggc cgcggaggag gccggctcgt cgtcgtccag gagcgagctg 360gatccagagc tcggcttcct tgccgaccac tccaaggccg agatcgtcga catgctccgg 420aagcacacct acgacgacga gctccgtcag ggcctgcgcc gcggccgcgg gcgcgcgcag 480ccgacgccgg cgtgggcacg agagctcctc ttcgagaagg ccgtgacccc gagcgacgtc 540ggcaagctca accgcctcgt ggtgccgaag cagcaggccg agaagcactt ccctccgacc 600actgcggcgg ccaccggcag caacggcaag ggcgtgctgc tcaacttcga ggacggcgaa 660gggaaggtgt ggcgcttccg gtactcgtac tggaacagca gccagagcta cgtgctcacc 720aagggctgga gccgcttcgt caaggagacg ggcctccgcg ccggcgacac cgtggcgttc 780taccggtcgg cgtacgggaa tgacacggag gatcagctct tcatcgacta caagaagatg 840aacaagaatg acgatgctgc ggacgcggcg atttccgatg agaatgagac aggccatgtc 900gccgtcaagc tcttcggcgt tgacattgcc ggtggaggga tggcgggatc atcaggtggc 960tga 963144320PRTTriticum aestivum 144Met Ala Ser Gly Lys Pro Thr Asn His Gly Met Glu Asp Asp Asn Asp1 5 10 15Met Glu Tyr Ser Ser Ala Glu Ser Gly Ala Glu Asp Ala Ala Glu Pro 20 25 30Ser Ser Ser Pro Val Leu Ala Pro Pro Arg Ala Ala Pro Ser Ser Arg 35 40 45Phe Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile 50 55 60Tyr Glu Lys His Ser Arg Val Trp Leu Gly Thr Phe Pro Asp Glu Asp65 70 75 80Ala Ala Ala Arg Ala Tyr Asp Val Ala Ala Leu Arg Phe Arg Gly Pro 85 90 95Asp Ala Val Ile Asn His Gln Arg Pro Thr Ala Ala Glu Glu Ala Gly 100 105 110Ser Ser Ser Ser Arg Ser Glu Leu Asp Pro Glu Leu Gly Phe Leu Ala 115 120 125Asp His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Asp Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Arg Ala Gln145 150 155 160Pro Thr Pro Ala Trp Ala Arg Glu Leu Leu Phe Glu Lys Ala Val Thr 165 170 175Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln Gln 180 185 190Ala Glu Lys His Phe Pro Pro Thr Thr Ala Ala Ala Thr Gly Ser Asn 195 200 205Gly Lys Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp 210 215 220Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr225 230 235 240Lys Gly Trp Ser Arg Phe Val Lys Glu Thr Gly Leu Arg Ala Gly Asp 245 250 255Thr Val Ala Phe Tyr Arg Ser Ala Tyr Gly Asn Asp Thr Glu Asp Gln 260 265 270Leu Phe Ile Asp Tyr Lys Lys Met Asn Lys Asn Asp Asp Ala Ala Asp 275 280 285Ala Ala Ile Ser Asp Glu Asn Glu Thr Gly His Val Ala Val Lys Leu 290 295 300Phe Gly Val Asp Ile Ala Gly Gly Gly Met Ala Gly Ser Ser Gly Gly305 310 315 320145963DNATriticum aestivum 145atggcatctg gcaagccgac aaaccacggg atggaggacg acaacgacat ggagtactcc 60tccgcggaat cgggggccga ggacgcggcg gagccgtcgt cgtcgccggt gctggcgccg 120ccccgggcgg ctccatcgtc gcggttcaag ggcgtcgtgc cgcagcccaa cgggcggtgg 180ggagcgcaga tctacgagaa gcactcgcgg gtgtggctcg gaacgttccc cgacgaggac 240gccgccgcgc gcgcctacga cgtggccgcg ctccgcttcc gcggcccgga cgccgtcatc 300aaccaccagc gaccgacggc cgcggaggag gccggctcgt cgtcgtccag gagcgagctg 360gatccagagc tcggcttcct cgccgaccac tccaaggccg agatcgtcga catgctccgg 420aagcacacct acgacgacga gctccgtcag ggcctgcgcc gcggccgcgg gcgcgcgcag 480ccgacgccgg cgtgggcacg agagctcctc ttcgagaagg ccgtgacccc gagcgacgtc 540ggcaagctca accgcctcgt ggtgccgaag cagcaggccg agaagcactt ccctccgacc 600actgcggcgg ccaccggcag caacggcaag ggcgtgctgc tcaacttcga ggacggcgaa 660gggaaggtgt ggcgcttccg gtactcgtac tggaacagca gccagagcta cgtgctcacc 720aagggctgga gccgcttcgt caaggagacg ggcctccgcg ccggcgacac cgtggcgttc 780taccggtcgg cgtacgggaa tgacacggag gatcagctct tcatcgacta caagaagatg 840aacaagaatg acgatgctgc ggacgcggcg atttccgatg agaatgagac aggccatgtc 900gccgtcaagc tcttcggcgt tgacattgcc ggtggaggga tggcgggatc atcaggtggc 960tga 963146488DNAArtificial SequencegRNA sequence 146gacggccagt gccaagcttc tcggatccac tagtaacggc cgccagtgtg ctggaattgc 60ccttggatca tgaaccaacg gcctggctgt atttggtggt tgtgtaggga gatggggaga 120agaaaagccc gattctcttc gctgtgatgg gctggatgca tgcgggggag cgggaggccc 180aagtacgtgc acggtgagcg gcccacaggg cgagtgtgag cgcgagaggc gggaggaaca 240gtttagtacc acattgccca gctaactcga acgcgaccaa cttataaacc cgcgcgctgt 300cgcttgtgtg ggaaggaaga gacagattgg ttttagagct agaaatagca agttaaaata 360aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgtcccttc 420gaagggcaat tctgcagata tccatcacac tggcggccgc tcgaggtcga agcttgcatg 480cctgcagg 48814741DNAArtificial Sequenceprimer 147ggactggggt tgctcctggg acacaagcga cagcgcgcgg g 4114841DNAArtificial Sequenceprimer 148cccaggagca accccagtcc gttttagagc tagaaatagc a 4114942DNAArtificial Sequenceprimer 149tgctatttct agctctaaaa cacacaagcg acagcgcgcg gg 4215041DNAArtificial Sequenceprimer 150gcccctgacg cccagtgacg gttttagagc tagaaatagc a 4115141DNAArtificial Sequenceprimer 151gggggtgccc ctgggcgaga acacaagcga cagcgcgcgg g 4115241DNAArtificial Sequenceprimer 152tctcgcccag gggcaccccc gttttagagc tagaaatagc a 4115341DNAArtificial Sequenceprimer 153ctcgtagtgg tggtggtagt acacaagcga cagcgcgcgg g 4115441DNAArtificial Sequenceprimer 154actaccacca ccactacgag gttttagagc tagaaatagc a 4115515681DNAArtificial Sequencevectormisc_feature(5964)..(5984)/note="target sequence"misc_feature(6617)..(6637)/note="target sequence" 155aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240tgccaaatgt ttgaacgatc ggggaaattc gagctctatc gatcaatcag gatccttact 300ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct 360gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt 420ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga 480aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct 540ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt 600cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt 660gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct 720tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt 780ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga 840acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg 900cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca 960gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca 1020gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct 1080tctttctggc gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc 1140cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatgctc agcactttcc 1200gcacggtggc aaaatcccgg cccttatccc acacgatctc cccggtttcg ccgtttgtct 1260cgatcagagg ccgcttccgg atctcgccgt tggccagggt aatctcggtc ttgaaaaagt 1320tcatgatgtt gctgtagaag aagtacttgg cggtagcctt gccgatttcc tgctcgctct 1380tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac tcgctttcca 1440gcttagggta ctttttgatc agggcggttc ccacgacggc gttcaggtag gcgtcgtggg 1500cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc cggaaatcgg 1560acaccagctt ggacttcagg gtgatcactt tcacttcccg gatcagcttg tcattctcgt 1620cgtacttagt gttcatccgg gagtccagga tctgtgccac gtgctttgtg atctgccggg 1680tttccaccag ctgtctcttg atgaagccgg ccttatccag ttcgctcagg ccgcctctct 1740cggccttggt cagattgtcg aactttctct gggtaatcag cttggcgttc agcagctgcc 1800gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg ctcttgcccc 1860ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga gtcgtccttc agaaagctct 1920gaggcacgat atggtccaca tcgtagtcgg acagccggtt gatgtccagt tcctggtcca 1980cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc tgcagctggg 2040tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg ccctcttcga 2100tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg ttctctctgg 2160ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg agctcgtcca 2220ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc agattggcaa 2280tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg tcctctttaa 2340aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag ccgtcggact 2400tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg atcagcttcc 2460ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc actttgtcgt 2520cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc tcaaacagtg 2580tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc aggaagtcct 2640tgtccttgat aattttcagc agatcgtggt atgtgcccag ggaggcgttg aaccgatctt 2700ccacgccgga gatttccacg gagtcgaagc actcgatttt cttgaagtag tcctctttca 2760gctgcttcac ggtcactttc cggttggtct tgaacagcag gtccacgatg gcctttttct 2820gctcgccgct caggaaggcg ggctttctca ttccctcggt cacgtatttc actttggtca 2880gctcgttata cacggtgaag tactcgtaca gcaggctgtg cttgggcagc accttctcgt 2940tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg gaagcgccct 3000tgtccaccac ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt ctggtcatcc 3060aggcgaatct gctgtttccc ctggccagag ggcccacgta gtaggggatg cggaaggtca 3120ggatcttctc gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct tcctgccgcc 3180gcagaatggc gtgcagctct cccaggtgga tctggtgggg gatgctgccg ttgtcgaagg 3240tccgctgctt ccgcagcagg tcctctctgt tcagcttcac gagcagttcc tcggtgccgt 3300ccatcttttc caggatgggc ttgatgaact tgtagaactc ttcctggctg gctccgccgt 3360caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg tacttctcag 3420gcagctgctg ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc tcgtcgtatc 3480tcttgatcat agaggcgctc aggggggcct tggtgatctc ggtgttcact ctcaggatgt 3540cgctcagcag gatggcgtcg gacaggttct tggcggccag aaacaggtcg gcgtactggt 3600cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg ctcagctgca 3660gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg cccaggctca 3720gggcaatcag gtttccgaac aggccattct tcttctcgcc gggcagctgg gcgatcagat 3780tttccagccg tctgctcttg ctcagtctgg cagacaggat ggccttggcg tccacgccgc 3840tggcgttgat ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc tggatgaaca 3900gcttgtccac gtcgctgttg tcggggttca ggtcgccctc gatcaggaag tggccccgga 3960acttgatcat gtgggccagg gccagataga tcagccgcag gtcggccttg tcggtgctgt 4020ccaccagttt ctttctcagg tggtagatgg tggggtactt ctcgtggtag gccacctcgt 4080ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt atcctcttcc accaggaagg 4140actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct 4200cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca 4260gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc aggttcttct 4320tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact 4380cgtcggtgat cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact 4440tcttgtcggc tgctgggact ccgtggatac cgaccttccg cttcttcttt ggggccatct 4500tatcgtcatc gtctttgtaa tcaatatcat gatccttgta gtctccgtcg tggtccttat 4560agtccatctc gagtatcgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta 4620aaagaaatga tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt 4680gttttgtata tgttgtgttg aggtcgaggt cctctccaaa tgaaatgaac ttccttatat 4740agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat 4800atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct 4860cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc 4920ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac 4980aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata tcaccctttg 5040ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta 5100gacaagtgtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg aagacgtggt 5160tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 5220gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga 5280gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc 5340gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga 5400gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacctg 5460caggcatgcc tcggatccac tagtaacggc cgccagtgtg ctggaattgc ccttaagctt 5520cgttgaacaa cggaaactcg acttgccttc cgcacaatac atcatttctt cttagctttt 5580tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg 5640tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt 5700ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat 5760cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga 5820agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg 5880aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac 5940agctagagtc gaagtagtga tttccccacg tcactgggcg tcgttttaga gctagaaata 6000gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 6060tttttgtccc ttcgaagggc ctttctcaga tatccatcac actggcggcc gctcgaggtc 6120gctcggatcc actagtaacg gccgccagtg tgctggaatt gcccttaagc ttcgttgaac 6180aacggaaact cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct 6240tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc 6300gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca 6360ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc 6420ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc 6480catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat 6540cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag 6600tcgaagtagt gattcacacc ccatggccag gactgtttta gagctagaaa tagcaagtta 6660aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgtc 6720ccttcgaagg gcctttctca gatatccatc acactggcgg ccgctcgagg tcgaagcttg 6780gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 6840cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 6900cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat 6960cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg 7020gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag 7080gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa 7140gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 7200cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 7260ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 7320agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 7380gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 7440gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 7500ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 7560cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 7620tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 7680ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 7740ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 7800cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 7860ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 7920tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 7980acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 8040cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 8100gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 8160tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 8220ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 8280gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 8340gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 8400ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 8460gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 8520attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 8580caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 8640cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 8700attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 8760acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 8820tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 8880gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 8940tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 9000gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 9060cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 9120tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 9180taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 9240ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 9300acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 9360acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg 9420cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 9480acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 9540caaagaatcc

cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 9600cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 9660cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 9720gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 9780cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 9840ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 9900cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 9960ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 10020ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 10080cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 10140cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 10200cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 10260atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 10320tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 10380ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 10440atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 10500aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 10560tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 10620gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 10680tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 10740agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 10800tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 10860agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 10920gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10980tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 11040gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 11100ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 11160aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 11220cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 11280ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 11340ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 11400cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 11460actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 11520cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 11580tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 11640gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 11700caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 11760agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 11820tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 11880tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 11940gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 12000gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta 12060ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc 12120cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg 12180gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa 12240gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa 12300gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt 12360aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa 12420gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc 12480gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg 12540ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct 12600ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca 12660tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt 12720catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat 12780tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat 12840tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa 12900gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa 12960taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg 13020gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa 13080catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc 13140cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc 13200cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg 13260ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac 13320aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg 13380ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt 13440attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc 13500ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg 13560agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg 13620ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc 13680gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg 13740tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag 13800agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc 13860ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag 13920aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg 13980accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg 14040aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg 14100acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca 14160atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg 14220ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc 14280ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca 14340cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga 14400atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg 14460gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga 14520gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga 14580aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga 14640tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct 14700ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt 14760gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt 14820ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg 14880tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg 14940ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg 15000aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag 15060actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat 15120caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg 15180ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct 15240ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag 15300tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa 15360gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga 15420gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg 15480cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 15540gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 15600ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 15660acagctatga ccatgattac g 1568115615681DNAArtificial Sequencevectormisc_feature(5964)..(5984)/note="target sequence"misc_feature(6617)..(6637)/note="target sequence" 156aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240tgccaaatgt ttgaacgatc ggggaaattc gagctctatc gatcaatcag gatccttact 300ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct 360gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt 420ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga 480aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct 540ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt 600cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt 660gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct 720tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt 780ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga 840acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg 900cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca 960gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca 1020gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct 1080tctttctggc gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc 1140cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatgctc agcactttcc 1200gcacggtggc aaaatcccgg cccttatccc acacgatctc cccggtttcg ccgtttgtct 1260cgatcagagg ccgcttccgg atctcgccgt tggccagggt aatctcggtc ttgaaaaagt 1320tcatgatgtt gctgtagaag aagtacttgg cggtagcctt gccgatttcc tgctcgctct 1380tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac tcgctttcca 1440gcttagggta ctttttgatc agggcggttc ccacgacggc gttcaggtag gcgtcgtggg 1500cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc cggaaatcgg 1560acaccagctt ggacttcagg gtgatcactt tcacttcccg gatcagcttg tcattctcgt 1620cgtacttagt gttcatccgg gagtccagga tctgtgccac gtgctttgtg atctgccggg 1680tttccaccag ctgtctcttg atgaagccgg ccttatccag ttcgctcagg ccgcctctct 1740cggccttggt cagattgtcg aactttctct gggtaatcag cttggcgttc agcagctgcc 1800gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg ctcttgcccc 1860ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga gtcgtccttc agaaagctct 1920gaggcacgat atggtccaca tcgtagtcgg acagccggtt gatgtccagt tcctggtcca 1980cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc tgcagctggg 2040tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg ccctcttcga 2100tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg ttctctctgg 2160ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg agctcgtcca 2220ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc agattggcaa 2280tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg tcctctttaa 2340aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag ccgtcggact 2400tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg atcagcttcc 2460ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc actttgtcgt 2520cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc tcaaacagtg 2580tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc aggaagtcct 2640tgtccttgat aattttcagc agatcgtggt atgtgcccag ggaggcgttg aaccgatctt 2700ccacgccgga gatttccacg gagtcgaagc actcgatttt cttgaagtag tcctctttca 2760gctgcttcac ggtcactttc cggttggtct tgaacagcag gtccacgatg gcctttttct 2820gctcgccgct caggaaggcg ggctttctca ttccctcggt cacgtatttc actttggtca 2880gctcgttata cacggtgaag tactcgtaca gcaggctgtg cttgggcagc accttctcgt 2940tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg gaagcgccct 3000tgtccaccac ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt ctggtcatcc 3060aggcgaatct gctgtttccc ctggccagag ggcccacgta gtaggggatg cggaaggtca 3120ggatcttctc gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct tcctgccgcc 3180gcagaatggc gtgcagctct cccaggtgga tctggtgggg gatgctgccg ttgtcgaagg 3240tccgctgctt ccgcagcagg tcctctctgt tcagcttcac gagcagttcc tcggtgccgt 3300ccatcttttc caggatgggc ttgatgaact tgtagaactc ttcctggctg gctccgccgt 3360caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg tacttctcag 3420gcagctgctg ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc tcgtcgtatc 3480tcttgatcat agaggcgctc aggggggcct tggtgatctc ggtgttcact ctcaggatgt 3540cgctcagcag gatggcgtcg gacaggttct tggcggccag aaacaggtcg gcgtactggt 3600cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg ctcagctgca 3660gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg cccaggctca 3720gggcaatcag gtttccgaac aggccattct tcttctcgcc gggcagctgg gcgatcagat 3780tttccagccg tctgctcttg ctcagtctgg cagacaggat ggccttggcg tccacgccgc 3840tggcgttgat ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc tggatgaaca 3900gcttgtccac gtcgctgttg tcggggttca ggtcgccctc gatcaggaag tggccccgga 3960acttgatcat gtgggccagg gccagataga tcagccgcag gtcggccttg tcggtgctgt 4020ccaccagttt ctttctcagg tggtagatgg tggggtactt ctcgtggtag gccacctcgt 4080ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt atcctcttcc accaggaagg 4140actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct 4200cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca 4260gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc aggttcttct 4320tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact 4380cgtcggtgat cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact 4440tcttgtcggc tgctgggact ccgtggatac cgaccttccg cttcttcttt ggggccatct 4500tatcgtcatc gtctttgtaa tcaatatcat gatccttgta gtctccgtcg tggtccttat 4560agtccatctc gagtatcgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta 4620aaagaaatga tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt 4680gttttgtata tgttgtgttg aggtcgaggt cctctccaaa tgaaatgaac ttccttatat 4740agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat 4800atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct 4860cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc 4920ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac 4980aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata tcaccctttg 5040ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta 5100gacaagtgtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg aagacgtggt 5160tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 5220gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga 5280gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc 5340gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga 5400gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacctg 5460caggcatgcc tcggatccac tagtaacggc cgccagtgtg ctggaattgc ccttaagctt 5520cgttgaacaa cggaaactcg acttgccttc cgcacaatac atcatttctt cttagctttt 5580tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg 5640tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt 5700ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat 5760cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga 5820agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg 5880aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac 5940agctagagtc gaagtagtga ttgcggagac tcgtctacag ttgttttaga gctagaaata 6000gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 6060tttttgtccc ttcgaagggc ctttctcaga tatccatcac actggcggcc gctcgaggtc 6120gctcggatcc actagtaacg gccgccagtg tgctggaatt gcccttaagc ttcgttgaac 6180aacggaaact cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct 6240tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc 6300gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca 6360ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc 6420ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc 6480catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat 6540cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag 6600tcgaagtagt gattatgtgt tacagcacgt cggggtttta gagctagaaa tagcaagtta 6660aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgtc 6720ccttcgaagg gcctttctca gatatccatc acactggcgg ccgctcgagg tcgaagcttg 6780gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 6840cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 6900cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat 6960cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg 7020gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag 7080gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa 7140gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 7200cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 7260ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 7320agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 7380gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 7440gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 7500ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 7560cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 7620tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 7680ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 7740ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 7800cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 7860ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 7920tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 7980acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 8040cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 8100gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 8160tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 8220ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 8280gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 8340gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 8400ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 8460gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 8520attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 8580caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 8640cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 8700attgaggtca

cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 8760acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 8820tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 8880gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 8940tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 9000gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 9060cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 9120tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 9180taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 9240ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 9300acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 9360acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg 9420cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 9480acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 9540caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 9600cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 9660cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 9720gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 9780cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 9840ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 9900cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 9960ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 10020ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 10080cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 10140cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 10200cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 10260atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 10320tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 10380ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 10440atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 10500aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 10560tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 10620gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 10680tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 10740agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 10800tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 10860agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 10920gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10980tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 11040gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 11100ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 11160aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 11220cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 11280ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 11340ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 11400cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 11460actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 11520cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 11580tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 11640gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 11700caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 11760agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 11820tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 11880tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 11940gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 12000gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta 12060ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc 12120cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg 12180gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa 12240gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa 12300gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt 12360aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa 12420gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc 12480gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg 12540ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct 12600ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca 12660tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt 12720catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat 12780tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat 12840tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa 12900gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa 12960taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg 13020gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa 13080catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc 13140cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc 13200cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg 13260ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac 13320aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg 13380ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt 13440attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc 13500ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg 13560agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg 13620ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc 13680gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg 13740tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag 13800agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc 13860ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag 13920aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg 13980accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg 14040aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg 14100acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca 14160atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg 14220ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc 14280ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca 14340cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga 14400atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg 14460gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga 14520gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga 14580aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga 14640tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct 14700ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt 14760gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt 14820ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg 14880tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg 14940ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg 15000aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag 15060actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat 15120caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg 15180ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct 15240ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag 15300tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa 15360gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga 15420gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg 15480cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 15540gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 15600ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 15660acagctatga ccatgattac g 1568115715681DNAArtificial Sequencevectormisc_feature(5964)..(5984)/note="target sequence"misc_feature(6617)..(6637)/note="target sequence" 157aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240tgccaaatgt ttgaacgatc ggggaaattc gagctctatc gatcaatcag gatccttact 300ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct 360gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt 420ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga 480aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct 540ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt 600cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt 660gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct 720tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt 780ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga 840acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg 900cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca 960gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca 1020gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct 1080tctttctggc gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc 1140cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatgctc agcactttcc 1200gcacggtggc aaaatcccgg cccttatccc acacgatctc cccggtttcg ccgtttgtct 1260cgatcagagg ccgcttccgg atctcgccgt tggccagggt aatctcggtc ttgaaaaagt 1320tcatgatgtt gctgtagaag aagtacttgg cggtagcctt gccgatttcc tgctcgctct 1380tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac tcgctttcca 1440gcttagggta ctttttgatc agggcggttc ccacgacggc gttcaggtag gcgtcgtggg 1500cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc cggaaatcgg 1560acaccagctt ggacttcagg gtgatcactt tcacttcccg gatcagcttg tcattctcgt 1620cgtacttagt gttcatccgg gagtccagga tctgtgccac gtgctttgtg atctgccggg 1680tttccaccag ctgtctcttg atgaagccgg ccttatccag ttcgctcagg ccgcctctct 1740cggccttggt cagattgtcg aactttctct gggtaatcag cttggcgttc agcagctgcc 1800gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg ctcttgcccc 1860ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga gtcgtccttc agaaagctct 1920gaggcacgat atggtccaca tcgtagtcgg acagccggtt gatgtccagt tcctggtcca 1980cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc tgcagctggg 2040tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg ccctcttcga 2100tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg ttctctctgg 2160ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg agctcgtcca 2220ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc agattggcaa 2280tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg tcctctttaa 2340aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag ccgtcggact 2400tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg atcagcttcc 2460ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc actttgtcgt 2520cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc tcaaacagtg 2580tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc aggaagtcct 2640tgtccttgat aattttcagc agatcgtggt atgtgcccag ggaggcgttg aaccgatctt 2700ccacgccgga gatttccacg gagtcgaagc actcgatttt cttgaagtag tcctctttca 2760gctgcttcac ggtcactttc cggttggtct tgaacagcag gtccacgatg gcctttttct 2820gctcgccgct caggaaggcg ggctttctca ttccctcggt cacgtatttc actttggtca 2880gctcgttata cacggtgaag tactcgtaca gcaggctgtg cttgggcagc accttctcgt 2940tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg gaagcgccct 3000tgtccaccac ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt ctggtcatcc 3060aggcgaatct gctgtttccc ctggccagag ggcccacgta gtaggggatg cggaaggtca 3120ggatcttctc gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct tcctgccgcc 3180gcagaatggc gtgcagctct cccaggtgga tctggtgggg gatgctgccg ttgtcgaagg 3240tccgctgctt ccgcagcagg tcctctctgt tcagcttcac gagcagttcc tcggtgccgt 3300ccatcttttc caggatgggc ttgatgaact tgtagaactc ttcctggctg gctccgccgt 3360caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg tacttctcag 3420gcagctgctg ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc tcgtcgtatc 3480tcttgatcat agaggcgctc aggggggcct tggtgatctc ggtgttcact ctcaggatgt 3540cgctcagcag gatggcgtcg gacaggttct tggcggccag aaacaggtcg gcgtactggt 3600cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg ctcagctgca 3660gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg cccaggctca 3720gggcaatcag gtttccgaac aggccattct tcttctcgcc gggcagctgg gcgatcagat 3780tttccagccg tctgctcttg ctcagtctgg cagacaggat ggccttggcg tccacgccgc 3840tggcgttgat ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc tggatgaaca 3900gcttgtccac gtcgctgttg tcggggttca ggtcgccctc gatcaggaag tggccccgga 3960acttgatcat gtgggccagg gccagataga tcagccgcag gtcggccttg tcggtgctgt 4020ccaccagttt ctttctcagg tggtagatgg tggggtactt ctcgtggtag gccacctcgt 4080ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt atcctcttcc accaggaagg 4140actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct 4200cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca 4260gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc aggttcttct 4320tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact 4380cgtcggtgat cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact 4440tcttgtcggc tgctgggact ccgtggatac cgaccttccg cttcttcttt ggggccatct 4500tatcgtcatc gtctttgtaa tcaatatcat gatccttgta gtctccgtcg tggtccttat 4560agtccatctc gagtatcgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta 4620aaagaaatga tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt 4680gttttgtata tgttgtgttg aggtcgaggt cctctccaaa tgaaatgaac ttccttatat 4740agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat 4800atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct 4860cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc 4920ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac 4980aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata tcaccctttg 5040ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta 5100gacaagtgtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg aagacgtggt 5160tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 5220gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga 5280gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc 5340gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga 5400gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacctg 5460caggcatgcc tcggatccac tagtaacggc cgccagtgtg ctggaattgc ccttaagctt 5520cgttgaacaa cggaaactcg acttgccttc cgcacaatac atcatttctt cttagctttt 5580tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg 5640tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt 5700ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat 5760cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga 5820agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg 5880aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac 5940agctagagtc gaagtagtga ttgcggagac tcgtctacag ttgttttaga gctagaaata 6000gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 6060tttttgtccc ttcgaagggc ctttctcaga tatccatcac actggcggcc gctcgaggtc 6120gctcggatcc actagtaacg gccgccagtg tgctggaatt gcccttaagc ttcgttgaac 6180aacggaaact cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct 6240tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc 6300gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca 6360ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc 6420ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc 6480catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat 6540cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag 6600tcgaagtagt gattttggtc tacggagcga tggtgtttta gagctagaaa tagcaagtta 6660aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgtc 6720ccttcgaagg gcctttctca gatatccatc acactggcgg ccgctcgagg tcgaagcttg 6780gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 6840cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 6900cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat 6960cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg 7020gtaaacctaa gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag 7080gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa 7140gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 7200cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 7260ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 7320agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 7380gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 7440gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 7500ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 7560cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 7620tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 7680ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 7740ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 7800cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 7860ctgcactgct

tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 7920tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 7980acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 8040cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 8100gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 8160tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 8220ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 8280gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 8340gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 8400ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 8460gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 8520attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 8580caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 8640cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 8700attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 8760acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 8820tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 8880gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 8940tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 9000gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 9060cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 9120tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 9180taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 9240ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 9300acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 9360acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg 9420cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 9480acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 9540caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 9600cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 9660cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 9720gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 9780cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 9840ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 9900cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 9960ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 10020ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 10080cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 10140cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 10200cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 10260atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 10320tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 10380ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 10440atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 10500aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 10560tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 10620gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 10680tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 10740agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 10800tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 10860agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 10920gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10980tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 11040gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 11100ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 11160aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 11220cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 11280ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 11340ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 11400cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 11460actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 11520cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 11580tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 11640gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 11700caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 11760agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 11820tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 11880tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 11940gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 12000gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta 12060ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc 12120cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg 12180gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa 12240gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa 12300gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt 12360aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa 12420gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc 12480gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg 12540ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct 12600ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca 12660tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt 12720catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat 12780tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat 12840tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa 12900gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa 12960taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg 13020gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa 13080catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc 13140cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc 13200cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg 13260ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac 13320aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg 13380ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt 13440attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc 13500ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg 13560agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg 13620ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc 13680gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg 13740tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag 13800agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc 13860ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag 13920aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg 13980accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg 14040aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg 14100acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca 14160atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg 14220ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc 14280ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca 14340cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga 14400atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg 14460gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga 14520gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga 14580aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga 14640tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct 14700ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt 14760gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt 14820ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg 14880tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg 14940ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg 15000aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag 15060actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat 15120caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg 15180ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct 15240ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag 15300tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa 15360gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga 15420gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg 15480cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 15540gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 15600ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 15660acagctatga ccatgattac g 1568115815681DNAArtificial Sequencevectormisc_feature(5964)..(5984)/note="target sequence"misc_feature(6617)..(6637)/note="target sequence" 158aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc 60tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc 120catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac 180agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat 240tgccaaatgt ttgaacgatc ggggaaattc gagctctatc gatcaatcag gatccttact 300ttttcttttt tgcctggccg gcctttttcg tggccgccgg ccttttgtcg cctcccagct 360gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt 420ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga 480aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct 540ctctgatggg cttatcccgg tgcttgttgt aggcggacag cactttgtcc agattagcgt 600cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt 660gcttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct 720tctcatagtg gctggccagg tacaggaagt tcacatattt ggagggcagg gccagttcgt 780ttcccttctg cagttcgccg gcagaggcca gcattctctt ccggccgttt tccagctcga 840acagggagta cttaggcagc ttgatgatca ggtccttttt cacttctttg tagcccttgg 900cttccagaaa gtcgatggga ttcttctcga agctgcttct ttccatgatg gtgatcccca 960gcagctcttt cacactcttc agtttcttgg acttgccctt ttccactttg gccaccacca 1020gcacagaata ggccacggtg gggctgtcga agccgccgta cttcttaggg tcccagtcct 1080tctttctggc gatcagctta tcgctgttcc tcttgggcag gatagactct ttgctgaagc 1140cgcctgtctg cacctcggtc tttttcacga tattcacttg gggcatgctc agcactttcc 1200gcacggtggc aaaatcccgg cccttatccc acacgatctc cccggtttcg ccgtttgtct 1260cgatcagagg ccgcttccgg atctcgccgt tggccagggt aatctcggtc ttgaaaaagt 1320tcatgatgtt gctgtagaag aagtacttgg cggtagcctt gccgatttcc tgctcgctct 1380tggcgatcat cttccgcacg tcgtacacct tgtagtcgcc gtacacgaac tcgctttcca 1440gcttagggta ctttttgatc agggcggttc ccacgacggc gttcaggtag gcgtcgtggg 1500cgtggtggta gttgttgatc tcgcgcactt tgtaaaactg gaaatccttc cggaaatcgg 1560acaccagctt ggacttcagg gtgatcactt tcacttcccg gatcagcttg tcattctcgt 1620cgtacttagt gttcatccgg gagtccagga tctgtgccac gtgctttgtg atctgccggg 1680tttccaccag ctgtctcttg atgaagccgg ccttatccag ttcgctcagg ccgcctctct 1740cggccttggt cagattgtcg aactttctct gggtaatcag cttggcgttc agcagctgcc 1800gccagtagtt cttcatcttc ttcacgacct cttcggaggg cacgttgtcg ctcttgcccc 1860ggttcttgtc gcttctggtc agcaccttgt tgtcgatgga gtcgtccttc agaaagctct 1920gaggcacgat atggtccaca tcgtagtcgg acagccggtt gatgtccagt tcctggtcca 1980cgtacatatc ccgcccattc tgcaggtagt acaggtacag cttctcgttc tgcagctggg 2040tgttttccac ggggtgttct ttcaggatct ggctgcccag ctctttgatg ccctcttcga 2100tccgcttcat tctctcgcgg ctgttcttct gtcccttctg ggtggtctgg ttctctctgg 2160ccatttcgat cacgatgttc tcgggcttgt gccggcccat cactttcacg agctcgtcca 2220ccaccttcac tgtctgcagg atgcccttct taatggcggg gctgccggcc agattggcaa 2280tgtgctcgtg caggctatcg ccctggccgg acacctgggc tttctggatg tcctctttaa 2340aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct gttggcgaag ccgtcggact 2400tcaggaaatc caggattgtc ttgccggact gcttgtcccg gatgccgttg atcagcttcc 2460ggctcagcct gccccagccg gtgtatctcc gccgcttcag ctgcttcatc actttgtcgt 2520cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat ctctctgtcc tcaaacagtg 2580tcagggtcag cacgatatct tccagaatgt cctcgttttc ctcattgtcc aggaagtcct 2640tgtccttgat aattttcagc agatcgtggt atgtgcccag ggaggcgttg aaccgatctt 2700ccacgccgga gatttccacg gagtcgaagc actcgatttt cttgaagtag tcctctttca 2760gctgcttcac ggtcactttc cggttggtct tgaacagcag gtccacgatg gcctttttct 2820gctcgccgct caggaaggcg ggctttctca ttccctcggt cacgtatttc actttggtca 2880gctcgttata cacggtgaag tactcgtaca gcaggctgtg cttgggcagc accttctcgt 2940tgggcaggtt cttatcgaag ttggtcatcc gctcgatgaa gctctgggcg gaagcgccct 3000tgtccaccac ttcctcgaag ttccaggggg tgatggtttc ctcgctcttt ctggtcatcc 3060aggcgaatct gctgtttccc ctggccagag ggcccacgta gtaggggatg cggaaggtca 3120ggatcttctc gatcttttcc cggttgtcct tcaggaatgg gtaaaaatct tcctgccgcc 3180gcagaatggc gtgcagctct cccaggtgga tctggtgggg gatgctgccg ttgtcgaagg 3240tccgctgctt ccgcagcagg tcctctctgt tcagcttcac gagcagttcc tcggtgccgt 3300ccatcttttc caggatgggc ttgatgaact tgtagaactc ttcctggctg gctccgccgt 3360caatgtagcc ggcgtagccg ttcttgctct ggtcgaagaa aatctctttg tacttctcag 3420gcagctgctg ccgcacgaga gctttcagca gggtcaggtc ctggtggtgc tcgtcgtatc 3480tcttgatcat agaggcgctc aggggggcct tggtgatctc ggtgttcact ctcaggatgt 3540cgctcagcag gatggcgtcg gacaggttct tggcggccag aaacaggtcg gcgtactggt 3600cgccgatctg ggccagcagg ttgtccaggt cgtcgtcgta ggtgtccttg ctcagctgca 3660gtttggcatc ctcggccagg tcgaagttgc tcttgaagtt gggggtcagg cccaggctca 3720gggcaatcag gtttccgaac aggccattct tcttctcgcc gggcagctgg gcgatcagat 3780tttccagccg tctgctcttg ctcagtctgg cagacaggat ggccttggcg tccacgccgc 3840tggcgttgat ggggttttcc tcgaacagct ggttgtaggt ctgcaccagc tggatgaaca 3900gcttgtccac gtcgctgttg tcggggttca ggtcgccctc gatcaggaag tggccccgga 3960acttgatcat gtgggccagg gccagataga tcagccgcag gtcggccttg tcggtgctgt 4020ccaccagttt ctttctcagg tggtagatgg tggggtactt ctcgtggtag gccacctcgt 4080ccacgatgtt gccgaagatg gggtgccgct cgtgcttctt atcctcttcc accaggaagg 4140actcttccag tctgtggaag aagctgtcgt ccaccttggc catctcgttg ctgaagatct 4200cttgcagata gcagatccgg ttcttccgtc tggtgtatct tcttctggcg gttctcttca 4260gccgggtggc ctcggctgtt tcgccgctgt cgaacagcag ggctccgatc aggttcttct 4320tgatgctgtg ccggtcggtg ttgcccagca ccttgaattt cttgctgggc accttgtact 4380cgtcggtgat cacggcccag cccacagagt tggtgccgat gtccaggccg atgctgtact 4440tcttgtcggc tgctgggact ccgtggatac cgaccttccg cttcttcttt ggggccatct 4500tatcgtcatc gtctttgtaa tcaatatcat gatccttgta gtctccgtcg tggtccttat 4560agtccatctc gagtatcgtt cgtaaatggt gaaaattttc agaaaattgc ttttgcttta 4620aaagaaatga tttaaattgc tgcaatagaa gtagaatgct tgattgcttg agattcgttt 4680gttttgtata tgttgtgttg aggtcgaggt cctctccaaa tgaaatgaac ttccttatat 4740agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat 4800atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct 4860cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc 4920ctttccttta tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac 4980aataaagtga cagatagctg ggcaatggaa tccgaggagg tttccggata tcaccctttg 5040ttgaaaagtc tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta 5100gacaagtgtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg aagacgtggt 5160tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 5220gtcggcagag gcatcttcaa cgatggcctt tcctttatcg caatgatggc atttgtagga 5280gccaccttcc ttttccacta tcttcacaat aaagtgacag atagctgggc aatggaatcc 5340gaggaggttt ccggatatta ccctttgttg aaaagtctca attgcccttt ggtcttctga 5400gactgtatct ttgatatttt tggagtagac aagtgtgtcg tgctccacca tgttgacctg 5460caggcatgcc tcggatccac tagtaacggc cgccagtgtg ctggaattgc ccttaagctt 5520cgttgaacaa cggaaactcg acttgccttc cgcacaatac atcatttctt cttagctttt 5580tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg 5640tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt 5700ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat 5760cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga 5820agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg 5880aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac 5940agctagagtc gaagtagtga tttccccacg tcactgggcg tcgttttaga gctagaaata 6000gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt 6060tttttgtccc ttcgaagggc ctttctcaga tatccatcac actggcggcc gctcgaggtc 6120gctcggatcc actagtaacg gccgccagtg tgctggaatt gcccttaagc ttcgttgaac 6180aacggaaact cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct 6240tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc 6300gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca 6360ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc 6420ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc 6480catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat 6540cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag 6600tcgaagtagt gattttggtc tacggagcga tggtgtttta gagctagaaa tagcaagtta 6660aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgtc 6720ccttcgaagg gcctttctca gatatccatc acactggcgg ccgctcgagg tcgaagcttg 6780gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 6840cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 6900cgcccttccc aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat 6960cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg 7020gtaaacctaa

gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag 7080gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa 7140gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 7200cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 7260ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 7320agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 7380gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 7440gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 7500ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 7560cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 7620tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 7680ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 7740ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 7800cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 7860ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 7920tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 7980acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 8040cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 8100gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 8160tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg 8220ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 8280gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 8340gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 8400ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 8460gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 8520attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 8580caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 8640cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 8700attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 8760acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 8820tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 8880gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 8940tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 9000gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 9060cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 9120tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 9180taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 9240ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 9300acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 9360acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg 9420cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca 9480acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg 9540caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg 9600cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg 9660cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt 9720gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc 9780cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa 9840ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga 9900cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac 9960ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg 10020ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag 10080cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat 10140cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc 10200cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag 10260atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg 10320tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga 10380ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc 10440atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa 10500aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat 10560tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat 10620gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact 10680tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga 10740agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg 10800tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc 10860agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct 10920gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 10980tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 11040gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 11100ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 11160aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 11220cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 11280ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 11340ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 11400cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 11460actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 11520cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 11580tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 11640gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 11700caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 11760agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 11820tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 11880tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 11940gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 12000gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta 12060ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc 12120cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg 12180gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa 12240gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa 12300gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt 12360aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa 12420gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc 12480gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg 12540ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct 12600ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca 12660tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt 12720catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat 12780tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat 12840tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa 12900gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa 12960taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg 13020gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa 13080catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc 13140cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc 13200cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg 13260ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac 13320aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg 13380ggatctggat tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt 13440attttacaaa tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc 13500ctataggaac cctaattccc ttatctggga actactcaca cattattatg gagaaactcg 13560agcttgtcga tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg 13620ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc 13680gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg 13740tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag 13800agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc 13860ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag 13920aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg 13980accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg 14040aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg 14100acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca 14160atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg 14220ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc 14280ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca 14340cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga 14400atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg 14460gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga 14520gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga 14580aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga 14640tctgcgaaag ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct 14700ctccaaatga aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt 14760gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt 14820ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg 14880tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg 14940ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg 15000aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag 15060actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat 15120caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg 15180ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct 15240ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag 15300tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa 15360gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga 15420gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg cctctccccg 15480cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 15540gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact 15600ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa 15660acagctatga ccatgattac g 1568115920DNAArtificial Sequencetarget site 159tccccacgtc actgggcgtc 2016020DNAArtificial Sequencetarget site 160cacaccccat ggccaggact 2016120DNAArtificial Sequencetarget site 161gcggagactc gtctacagtt 2016220DNAArtificial Sequencetarget site 162atgtgttaca gcacgtcggg 2016320DNAArtificial Sequencevector 163ttggtctacg gagcgatggt 2016410PRTArtificial Sequencealignment 164Asp Arg Leu Phe Ile Asp Trp Lys Arg Arg1 5 101659PRTArtificial Sequencealignment 165Leu Arg Leu Phe Gly Val Asp Val Glu1 51669PRTArtificial Sequencealignment 166Leu Arg Leu Phe Gly Val Asp Met Glu1 51679PRTArtificial Sequencealignment 167Leu Arg Leu Phe Gly Val Asp Met Glu1 51689PRTArtificial Sequencealignment 168Leu Arg Leu Phe Gly Val Asp Met Glu1 51699PRTArtificial Sequencealignment 169Leu Arg Leu Phe Gly Val Asp Met Glu1 51709PRTArtificial Sequencealignment 170Leu Arg Leu Phe Gly Val Asp Met Glu1 51719PRTArtificial Sequencealignment 171Leu Arg Leu Phe Gly Val Asp Met Glu1 51729PRTArtificial Sequencealignment 172Leu Arg Leu Phe Gly Val Asn Met Glu1 51739PRTArtificial Sequencealignment 173Leu Arg Leu Phe Gly Val Asn Met Glu1 51749PRTArtificial Sequencealignment 174Leu Arg Leu Phe Gly Val Asn Met Glu1 51759PRTArtificial Sequencealignment 175Leu Arg Leu Phe Gly Val Asn Met Glu1 51769PRTArtificial Sequencealignment 176Leu Arg Leu Phe Gly Val Asn Met Glu1 51779PRTArtificial Sequencealignment 177Leu Arg Leu Phe Gly Val Asn Met Glu1 51789PRTArtificial Sequencealignment 178Leu Arg Leu Phe Gly Val Cys Ile Thr1 51799PRTArtificial Sequencealignment 179Val Arg Leu Phe Gly Val Asp Ile Ala1 51809PRTArtificial Sequencealignment 180Val Arg Leu Phe Gly Val Asp Ile Ala1 51819PRTArtificial Sequencealignment 181Val Arg Leu Phe Gly Val Asp Ile Phe1 51829PRTArtificial Sequencealignment 182Val Arg Leu Phe Gly Val Asp Ile Ser1 51839PRTArtificial Sequencealignment 183Val Arg Leu Phe Gly Val Asn Ile Leu1 51849PRTArtificial Sequencealignment 184Val Arg Leu Phe Gly Val Asn Ile Leu1 51859PRTArtificial Sequencealignment 185Val Arg Leu Phe Gly Val Asp Leu Leu1 51869PRTArtificial Sequencealignment 186Val Arg Leu Phe Gly Val Asp Leu Leu1 51879PRTArtificial Sequencealignment 187Val Arg Leu Phe Gly Val Asp Leu Leu1 51889PRTArtificial Sequencealignment 188Val Arg Leu Phe Gly Val Asp Leu Leu1 51899PRTArtificial Sequencealignment 189Val Arg Leu Phe Gly Val Asn Leu Leu1 51909PRTArtificial Sequencealignment 190Val Arg Leu Phe Gly Val Asn Leu Glu1 51919PRTArtificial Sequencealignment 191Val Arg Leu Phe Gly Val Asn Leu Glu1 51929PRTArtificial Sequencealignment 192Val Arg Leu Phe Gly Val Asn Leu Glu1 51939PRTArtificial Sequencealignment 193Val Arg Leu Phe Gly Val Asn Leu Glu1 51949PRTArtificial Sequencealignment 194Val Arg Leu Phe Gly Val Asn Leu Glu1 51959PRTArtificial Sequencealignment 195Leu Arg Leu Phe Gly Val Asn Leu Asp1 51969PRTArtificial Sequencealignment 196Leu Arg Leu Phe Gly Val Asn Leu Asp1 51979PRTArtificial Sequencealignment 197Val Arg Leu Phe Gly Val Asn Leu Asp1 51989PRTArtificial Sequencealignment 198Val Arg Leu Phe Gly Val Asn Leu Asp1 51999PRTArtificial Sequencealignment 199Val Arg Leu Phe Gly Val Asn Leu Asp1 52009PRTArtificial Sequencealignment 200Val Arg Leu Phe Gly Val Asn Leu Asp1 52019PRTArtificial Sequencealignment 201Val Arg Leu Phe Gly Val Asn Leu Asp1 52029PRTArtificial Sequencealignment 202Val Arg Leu Phe Gly Val Asn Leu Asp1 52039PRTArtificial Sequencealignment 203Val Arg Leu Phe Gly Val Asn Leu Asp1 52049PRTArtificial Sequencealignment 204Val Arg Leu Phe Gly Val Asn Leu Asp1 52059PRTArtificial Sequencealignment 205Val Arg Leu Phe Gly Val Asn Leu Asp1 5206894DNABrassica rapa 206atgatgatga caaacttgtc tctttcaaga gaaggagaag aggaggaaga agaagaacaa 60gaagaggcca agaagcccat ggaagaagta gagagagagc acatgttcga caaagtggtg 120actccaagcg atgttggtaa actaaaccgg ctcgtgatcc caaagcaata cgcagagaga 180tacttccctt tagattcatc cacaaacgag aaaggtttgc ttctaaactt cgaagatctc 240gcaggaaagt catggaggtt ccgttactct tactggaaca gtagtcagag ctatgtcatg 300actaaaggtt ggagccgttt cgttaaagac aaaaagctag acgccggaga tattgtctct 360ttccagagat gtgtcggaga ttcaggaaga gacagccgct tgtttattga ttggaggaga 420agacctaaag ttcctgacca tccgacatcg attgctcact ttgctgccgg atctatgttt 480cctaggtttt acagttttcc gacagcaact agttacaatc tttacaacta tcagcagcca 540cgtcatcatc atcacagtgg ttataattat cctcaaattc cgagagaatt tggatacggg 600tacttggtgg atcaaagagc cgtggtggct gatccgttgg tgattgaatc tgtgccggtg 660atgatgcacg gaggagctca agttagtcag gcggttgttg gaacggccgg gaagaggctg 720aggctttttg gagtcgatat ggaggaagaa tcttcatctt ccggtgggag tttgccacgt 780ggtgacgctt ctccgtcttc ctctttgttt cagctgagac ttggaagcag cagtgaagat 840gatcacttct ctaagaaagg aaagtcctca ttgccttttg atttggatca ataa 894207540PRTArtificial Sequencealignment 207Met Ala Ala Ser Pro Ser Ser Pro Leu Thr Ala Pro Pro Glu Pro Val1 5 10 15Thr Pro Pro Ser Pro Trp Thr Ile Thr Asp Gly Ala Ile Ser Gly Thr 20 25 30Leu Pro Ala Ala Glu Ala Phe Ala Val His Tyr Pro Gly Tyr Pro Ser 35 40 45Ser Pro Ala Arg Ala Ala Arg Thr Leu Gly Gly Leu Pro Gly Leu Ala 50 55 60Lys Val Arg Ser Ser Asp Pro Gly Ala Arg Leu Glu Leu Arg Phe Arg65 70 75 80Pro Glu Asp Pro Tyr Cys His Pro Ala Phe Gly Gln Ser Arg Ala Ser 85 90 95Thr Gly Leu Leu Leu Arg Leu Ser Lys Arg Lys Gly Ala Ala Ala Pro 100 105 110Cys Ala His Val Val Ala Arg Val Arg Thr Ala Tyr Tyr Phe Glu Gly 115 120 125Met Ala Asp Phe Gln His Val Val Pro Val His Ala Ala Gln Thr Arg 130 135 140Lys Arg Lys His Ser Asp Ser Gln Asn Asp Asn Glu Asn Phe Gly Ser145 150 155 160Asp Lys Thr Gly His Asp Glu Ala Asp Gly Asp Val Met Met Leu Val 165 170 175Pro Pro Leu Phe Ser Val Lys Asp Arg Pro Thr Lys Ile Ala Leu Val 180 185 190Pro Ser Ser Asn Ala Ile Ser Lys Thr Met His Arg Gly Val Val Gln 195 200 205Glu Arg Trp Glu Met Asn Val Gly Pro Thr Leu Ala Leu Pro Phe Asn 210 215 220Thr Gln Val Val Pro Glu Lys Ile Asn Trp Glu Asp His Ile Arg Lys225 230 235 240Asn Ser Val Glu Trp Gly Trp Gln Met Ala Val Cys Lys Leu Phe Asp

245 250 255Glu Arg Pro Val Trp Pro Arg Gln Ser Leu Tyr Glu Arg Phe Leu Asp 260 265 270Asp Asn Val His Val Ser Gln Asn Gln Phe Lys Arg Leu Leu Phe Arg 275 280 285Ala Gly Tyr Tyr Phe Ser Thr Gly Pro Phe Gly Lys Phe Trp Ile Arg 290 295 300Arg Gly Tyr Asp Pro Arg Lys Asp Ser Glu Ser Gln Ile Tyr Gln Arg305 310 315 320Ile Asp Phe Arg Met Pro Pro Glu Leu Arg Tyr Leu Leu Arg Leu Lys 325 330 335Asn Ser Glu Ser Arg Lys Trp Ala Asp Met Cys Lys Leu Glu Thr Met 340 345 350Pro Ser Gln Ser Phe Ile Tyr Leu Gln Leu Tyr Glu Leu Lys Asp Asp 355 360 365Phe Ile Gln Ala Glu Ile Arg Lys Pro Ser Tyr Gln Ser Val Cys Ser 370 375 380Arg Ser Thr Gly Trp Phe Ser Lys Pro Met Ile Lys Thr Leu Arg Leu385 390 395 400Gln Val Ser Ile Arg Leu Leu Ser Leu Leu His Asn Glu Glu Ala Lys 405 410 415Asn Leu Leu Arg Asn Ala His Glu Leu Ile Glu Arg Ser Lys Lys Gln 420 425 430Glu Ala Leu Ser Arg Ser Glu Leu Ser Ile Glu Tyr Asn Asp Ala Asp 435 440 445Gln Val Ser Ala Ala His Thr Gly Thr Glu Asp Gln Val Gly Pro Asn 450 455 460Asn Ser Asp Ser Glu Asp Val Asp Asp Glu Glu Glu Glu Glu Glu Leu465 470 475 480Glu Gly Tyr Asp Ser Pro Pro Met Ala Asp Asp Ile His Glu Phe Thr 485 490 495Leu Gly Asp Ser Tyr Ala Phe Gly Glu Gly Phe Ser Asn Gly Tyr Leu 500 505 510Glu Glu Val Leu Arg Ser Leu Pro Leu Gln Glu Asp Gly Gln Lys Lys 515 520 525Leu Cys Asp Ala Pro Ile Asn Ala Asp Ala Ser Asp 530 535 540208160PRTArtificial Sequencealignment 208Met Tyr Cys Ser Arg Gly Arg Ile Asp Pro Ala Glu Glu Gly Gln Val1 5 10 15Met Gly Gly Leu Gly Val Arg Asp Ala Ser Trp Ala Leu Phe Lys Val 20 25 30Leu Glu Gln Ser Asp Val Gln Val Gly Gln Asn Arg Leu Leu Leu Thr 35 40 45Lys Glu Ala Val Trp Gly Gly Pro Ile Pro Lys Leu Phe Pro Glu Leu 50 55 60Glu Glu Leu Arg Gly Asp Gly Leu Asn Ala Glu Asn Arg Val Ala Val65 70 75 80Lys Ile Leu Asp Ala Asp Gly Cys Glu Gly Asp Ala Asn Phe Arg Tyr 85 90 95Leu Asn Ser Ser Lys Ala Tyr Arg Val Met Gly Pro Gln Trp Ser Arg 100 105 110Leu Val Lys Glu Thr Gly Met Cys Lys Gly Asp Arg Leu Asp Leu Tyr 115 120 125Ala Ala Thr Ala Thr Ala Ala Ser Ser Cys Ser Gly Ala Arg Ala Ala 130 135 140Val Ala Pro Ala Ile Pro Pro Gly Ala Ile Val Lys Ala Ala Gly Phe145 150 155 160209192PRTArtificial Sequencealignment 209Met Ala Met His Ala Gly His Ala Trp Trp Gly Val Ala Met Tyr Thr1 5 10 15Asn His Tyr His His His Tyr Arg His Lys Thr Ser Asp Val Gly Lys 20 25 30Asn Arg Val Lys His Ala Arg Tyr Gly Gly Gly Asp Ser Gly Lys Gly 35 40 45Ser Asp Ser Gly Lys Trp Arg Arg Tyr Ser Tyr Trp Thr Ser Ser Ser 50 55 60Tyr Val Thr Lys Gly Trp Ser Arg Tyr Val Lys Lys Arg Asp Ala Gly65 70 75 80Asp Val Val His Arg Val Arg Gly Gly Ala Ala Asp Arg Gly Cys Arg 85 90 95Arg Arg Gly Ser Ala Ala Ala Val Arg Val Thr Ala Asn Gly Gly Trp 100 105 110Ser Met Cys Tyr Ser Thr Ser Gly Ser Ser Tyr Asp Thr Ser Ala Asn 115 120 125Ser Tyr Ala Tyr His Arg Ser Val Asp Asp His Ser Asp His Ala Gly 130 135 140Ser Arg Ala Asp Ala Lys Ser Ser Ser Ala Ala Ser Ala Ser Arg Arg145 150 155 160Arg Gly Val Asn Asp Cys Gly Ala Asp Ala Thr Ala Met Tyr Gly Tyr 165 170 175Met His His Ser Tyr Ala Ala Val Ser Thr Val Asn Tyr Trp Ser Val 180 185 190210491PRTArtificial Sequencealignment 210Met Glu Leu Met Gln Glu Val Lys Gly Tyr Ser Asp Gly Arg Glu Glu1 5 10 15Glu Glu Glu Glu Glu Glu Ala Ala Glu Glu Ile Ile Thr Arg Glu Glu 20 25 30Ser Ser Arg Leu Leu His Gln His Gln Glu Ala Ala Gly Ser Asn Phe 35 40 45Ile Ile Asn Asn Asn His His His His Gln His His His His His Thr 50 55 60Thr Lys Gln Leu Asp Phe Met Asp Leu Ser Leu Gly Ser Ser Lys Asp65 70 75 80Glu Gly Asn Leu Gln Gly Ser Ser Ser Ser Val Tyr Ala His His His 85 90 95His Ala Ala Ser Ala Ser Ser Ser Ala Asn Gly Asn Asn Asn Asn Ser 100 105 110Ser Ser Ser Asn Leu Gln Gln Gln Gln Gln Gln Pro Ala Glu Lys Glu 115 120 125His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn 130 135 140Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp145 150 155 160Ser Ser Ala Asn Glu Lys Gly Leu Leu Leu Asn Phe Glu Asp Arg Asn 165 170 175Gly Lys Leu Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser 180 185 190Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Lys Leu 195 200 205Asp Ala Gly Asp Met Val Ser Phe Gln Arg Gly Val Gly Glu Leu Tyr 210 215 220Arg His Arg Leu Tyr Ile Asp Trp Trp Arg Arg Pro Asp His His His225 230 235 240His His His His Gly Pro Asp His Ser Thr Thr Leu Phe Thr Pro Phe 245 250 255Leu Ile Pro Asn Gln Pro His His Leu Met Ser Ile Arg Trp Gly Ala 260 265 270Thr Gly Arg Leu Tyr Ser Leu Pro Ser Pro Thr Pro Pro Arg His His 275 280 285Glu His Leu Asn Tyr Asn Asn Asn Ala Met Tyr His Pro Phe His His 290 295 300His Gly Ala Gly Ser Gly Ile Asn Ala Thr Thr His His Tyr Asn Asn305 310 315 320Tyr His Glu Met Ser Ser Thr Thr Thr Ser Gly Ser Ala Gly Ser Val 325 330 335Phe Tyr His Arg Ser Thr Pro Pro Ile Ser Met Pro Leu Ala Asp His 340 345 350Gln Thr Leu Asn Thr Arg Gln Gln Gln Gln Gln Gln Gln Gln Gln Glu 355 360 365Gly Ala Gly Asn Val Ser Leu Ser Pro Met Ile Ile Asp Ser Val Pro 370 375 380Val Ala His His Leu His His Gln Gln His His Gly Gly Lys Ser Ser385 390 395 400Gly Pro Ser Ser Thr Ser Thr Ser Pro Ser Thr Ala Gly Lys Arg Leu 405 410 415Arg Leu Phe Gly Val Asn Met Glu Cys Ala Ser Ser Thr Ser Glu Asp 420 425 430Pro Lys Cys Phe Ser Leu Leu Ser Ser Ser Ser Met Ala Asn Ser Asn 435 440 445Ser Gln Pro Pro Leu Gln Leu Leu Arg Glu Asp Thr Leu Ser Ser Ser 450 455 460Ser Ala Arg Phe Gly Asp Gln Arg Gly Val Gly Glu Pro Ser Met Leu465 470 475 480Phe Asp Leu Asp Pro Ser Leu Gln Tyr Arg Gln 485 490211297PRTArtificial Sequencealignment 211Met Met Met Thr Asn Leu Ser Leu Ser Arg Glu Gly Glu Glu Glu Glu1 5 10 15Glu Glu Glu Gln Glu Glu Ala Lys Lys Pro Met Glu Glu Val Glu Arg 20 25 30Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu 35 40 45Asn Arg Leu Val Ile Pro Lys Gln Tyr Ala Glu Arg Tyr Phe Pro Leu 50 55 60Asp Ser Ser Thr Asn Glu Lys Gly Leu Leu Leu Asn Phe Glu Asp Leu65 70 75 80Ala Gly Lys Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln 85 90 95Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Asp Lys Lys 100 105 110Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Cys Val Gly Asp Ser 115 120 125Gly Arg Asp Ser Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Lys Val 130 135 140Pro Asp His Pro Thr Ser Ile Ala His Phe Ala Ala Gly Ser Met Phe145 150 155 160Pro Arg Phe Tyr Ser Phe Pro Thr Ala Thr Ser Tyr Asn Leu Tyr Asn 165 170 175Tyr Gln Gln Pro Arg His His His His Ser Gly Tyr Asn Tyr Pro Gln 180 185 190Ile Pro Arg Glu Phe Gly Tyr Gly Tyr Leu Val Asp Gln Arg Ala Val 195 200 205Val Ala Asp Pro Leu Val Ile Glu Ser Val Pro Val Met Met His Gly 210 215 220Gly Ala Gln Val Ser Gln Ala Val Val Gly Thr Ala Gly Lys Arg Leu225 230 235 240Arg Leu Phe Gly Val Asp Met Glu Glu Glu Ser Ser Ser Ser Gly Gly 245 250 255Ser Leu Pro Arg Gly Asp Ala Ser Pro Ser Ser Ser Leu Phe Gln Leu 260 265 270Arg Leu Gly Ser Ser Ser Glu Asp Asp His Phe Ser Lys Lys Gly Lys 275 280 285Ser Ser Leu Pro Phe Asp Leu Asp Gln 290 295212310PRTArtificial Sequencealignment 212Met Met Thr Asn Leu Ser Leu Ala Arg Glu Gly Glu Glu Glu Glu Glu1 5 10 15Glu Ala Gly Ala Lys Lys Pro Thr Glu Glu Val Glu Arg Glu His Met 20 25 30Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu 35 40 45Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Leu Asp Ser Ser 50 55 60Thr Asn Glu Lys Gly Leu Ile Leu Asn Phe Glu Asp Leu Thr Gly Lys65 70 75 80Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 85 90 95Met Thr Lys Gly Trp Ser Arg Phe Val Lys Asp Lys Lys Leu Asp Ala 100 105 110Gly Asp Ile Val Ser Phe Leu Arg Cys Val Gly Asp Thr Gly Arg Asp 115 120 125Ser Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Lys Val Pro Asp Tyr 130 135 140Thr Thr Ser Thr Ser His Phe Pro Ala Gly Ala Met Phe Pro Arg Phe145 150 155 160Tyr Ser Phe Gln Thr Ala Thr Thr Ser Thr Ser Tyr Asn Pro Tyr Asn 165 170 175His Gln Gln Pro Arg His His His Ser Gly Tyr Cys Tyr Pro Gln Ile 180 185 190Pro Arg Glu Phe Gly Tyr Gly Tyr Val Val Arg Ser Val Asp Gln Arg 195 200 205Ala Val Val Ala Asp Pro Leu Val Ile Glu Ser Val Pro Val Met Met 210 215 220His Gly Gly Ala Arg Val Asn Gln Ala Ala Val Gly Thr Ala Gly Lys225 230 235 240Arg Leu Arg Leu Phe Gly Val Asp Met Glu Cys Gly Glu Ser Gly Gly 245 250 255Thr Asn Ser Thr Glu Glu Glu Ser Ser Ser Ser Gly Gly Ser Leu Pro 260 265 270Arg Gly Gly Ala Ser Pro Ser Ser Ser Met Phe Gln Leu Arg Leu Gly 275 280 285Asn Ser Ser Glu Asp Asp His Leu Phe Lys Lys Gly Lys Ser Ser Leu 290 295 300Pro Phe Asn Leu Asp Gln305 310213293PRTArtificial Sequencealignment 213Met Met Thr Asn Leu Ser Leu Ala Arg Glu Gly Glu Ala Gln Val Lys1 5 10 15Lys Pro Ile Glu Glu Val Glu Arg Glu His Met Phe Asp Lys Val Val 20 25 30Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln 35 40 45His Ala Glu Arg Tyr Phe Pro Leu Asp Ser Ser Ser Asn Glu Lys Gly 50 55 60Leu Leu Leu Asn Phe Glu Asp Leu Thr Gly Lys Ser Trp Arg Phe Arg65 70 75 80Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp 85 90 95Ser Arg Phe Val Lys Asp Lys Lys Leu Asp Ala Gly Asp Ile Val Ser 100 105 110Phe Gln Arg Cys Val Gly Asp Ser Arg Leu Phe Ile Asp Trp Arg Arg 115 120 125Arg Pro Lys Val Pro Asp Tyr Pro Thr Ser Thr Ala His Phe Ala Ala 130 135 140Gly Ala Met Phe Pro Arg Phe Tyr Ser Phe Pro Thr Ala Thr Thr Ser145 150 155 160Thr Cys Tyr Asp Leu Tyr Asn His Gln Pro Pro Arg His His His Ile 165 170 175Gly Tyr Gly Tyr Pro Gln Ile Pro Arg Glu Phe Gly Tyr Gly Tyr Phe 180 185 190Val Arg Ser Val Asp Gln Arg Ala Val Val Ala Asp Pro Leu Val Ile 195 200 205Glu Ser Val Pro Val Met Met Arg Gly Gly Ala Arg Val Ser Gln Glu 210 215 220Val Val Gly Thr Ala Gly Lys Arg Leu Arg Leu Phe Gly Val Asp Met225 230 235 240Glu Glu Glu Ser Ser Ser Ser Gly Gly Ser Leu Pro Arg Ala Gly Gly 245 250 255Gly Gly Ala Ser Ser Ser Ser Ser Leu Phe Gln Leu Arg Leu Gly Ser 260 265 270Ser Cys Glu Asp Asp His Phe Ser Lys Lys Gly Lys Ser Ser Leu Pro 275 280 285Phe Asp Leu Asp Gln 290214320PRTArtificial Sequencealignment 214Met Glu Arg Lys Ser Asn Asp Leu Glu Arg Ser Glu Asn Ile Asp Ser1 5 10 15Gln Asn Lys Lys Met Asn Leu Glu Glu Glu Arg Pro Val Gln Glu Ala 20 25 30Ser Ser Met Glu Arg Glu His Met Phe Asp Lys Val Val Thr Pro Ser 35 40 45Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu 50 55 60Arg Tyr Phe Pro Leu Asp Asn Asn Ser Ser Asp Asn Asn Lys Gly Leu65 70 75 80Leu Leu Asn Phe Glu Asp Arg Ile Gly Ile Leu Trp Ser Phe Arg Tyr 85 90 95Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser 100 105 110Arg Phe Val Lys Asp Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe 115 120 125His Arg Gly Ser Cys Asn Lys Asp Lys Leu Phe Ile Asp Trp Lys Arg 130 135 140Arg Pro Lys Ile Pro Asp His Gln Val Val Gly Ala Met Phe Pro Arg145 150 155 160Phe Tyr Ser Tyr Pro Tyr Pro Gln Ile Gln Ala Ser Tyr Glu Arg His 165 170 175Asn Leu Tyr His Arg Tyr Gln Arg Asp Ile Gly Ile Gly Tyr Tyr Val 180 185 190Arg Ser Met Glu Arg Tyr Asp Pro Thr Ala Val Ile Glu Ser Val Pro 195 200 205Val Ile Met Gln Arg Arg Ala His Val Ala Thr Met Ala Ser Ser Arg 210 215 220Gly Glu Lys Arg Leu Arg Leu Phe Gly Val Asp Met Glu Cys Val Arg225 230 235 240Gly Gly Arg Gly Gly Gly Gly Ser Val Asn Ser Thr Glu Glu Glu Ser 245 250 255Ser Thr Ser Gly Gly Ser Ile Ser Arg Gly Gly Val Ser Met Ala Gly 260 265 270Val Gly Ser Pro Leu Gln Leu Arg Leu Val Ser Ser Asp Gly Asp Asp 275 280 285Gln Ser Leu Val Ala Arg Gly Ala Ala Arg Val Asp Glu Asp His His 290 295 300Leu Phe Thr Lys Lys Gly Lys Ser Ser Leu Ser Phe Asp Leu Asp Lys305 310 315 320215286PRTArtificial Sequencealignment 215Met Asn Gln Glu Glu Glu Asn Pro Val Glu Lys Ala Ser Ser Met Glu1 5 10 15Arg Glu His Met Phe Glu Lys Val Val Thr Pro Ser Asp Val Gly Lys 20 25 30Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro 35 40 45Leu Asp Asn Asn Ser Asp Ser Ser Lys Gly Leu Leu Leu Asn Phe Glu 50 55 60Asp Arg Thr Gly Asn Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser65 70 75 80Ser Gln Ser Tyr Val

Met Thr Lys Gly Trp Ser Arg Phe Val Lys Asp 85 90 95Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Asp Pro Gly 100 105 110Asn Lys Asp Lys Leu Phe Ile Asp Trp Arg Arg Arg Pro Lys Ile Pro 115 120 125Asp His His His Gln Phe Ala Gly Ala Met Phe Pro Arg Phe Tyr Ser 130 135 140Phe Ser His Pro Gln Asn Leu Tyr His Arg Tyr Gln Gln Asp Leu Gly145 150 155 160Ile Gly Tyr Tyr Val Ser Ser Met Glu Arg Asn Asp Pro Thr Ala Val 165 170 175Ile Glu Ser Val Pro Leu Ile Met Gln Arg Arg Ala Ala His Val Ala 180 185 190Ala Ile Pro Ser Ser Arg Gly Glu Lys Arg Leu Arg Leu Phe Gly Val 195 200 205Asp Met Glu Cys Gly Gly Gly Gly Gly Ser Val Asn Ser Thr Glu Glu 210 215 220Glu Ser Ser Ser Ser Gly Gly Gly Gly Gly Val Ser Met Ala Ser Val225 230 235 240Gly Ser Leu Leu Gln Leu Arg Leu Val Ser Ser Asp Asp Glu Ser Leu 245 250 255Val Ala Met Glu Ala Ala Ser Val Asp Glu Asp His His Leu Phe Thr 260 265 270Lys Lys Gly Lys Ser Ser Leu Ser Phe Asp Leu Asp Arg Lys 275 280 285216292PRTArtificial Sequencealignment 216Met Asn Gln Glu Asn Lys Lys Pro Leu Glu Glu Ala Ser Thr Ser Met1 5 10 15Glu Arg Glu Asn Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly 20 25 30Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 35 40 45Pro Leu Asp Asn Ser Ser Thr Asn Asn Lys Gly Leu Leu Leu Asp Phe 50 55 60Glu Asp Arg Thr Gly Ser Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn65 70 75 80Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys 85 90 95Asp Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Asp Pro 100 105 110Cys Asn Lys Asp Lys Leu Tyr Ile Asp Trp Arg Arg Arg Pro Lys Ile 115 120 125Pro Asp His His Gln Phe Ala Gly Ala Met Phe Pro Arg Phe Tyr Ser 130 135 140Phe Pro His Pro Gln Met Pro Thr Ser Phe Glu Ser Ser His Asn Leu145 150 155 160Tyr His His Arg Phe Gln Arg Asp Leu Gly Ile Gly Tyr Tyr Pro Thr 165 170 175Ala Val Ile Glu Ser Val Pro Val Ile Met Gln Arg Arg Glu Ala Gln 180 185 190Val Ala Asn Met Ala Ser Ser Arg Gly Glu Lys Arg Leu Arg Leu Phe 195 200 205Gly Val Asp Val Glu Cys Gly Gly Gly Gly Gly Gly Ser Val Asn Ser 210 215 220Thr Glu Glu Glu Ser Ser Ser Ser Gly Gly Ser Met Ser Arg Gly Gly225 230 235 240Val Ser Met Ala Gly Val Gly Ser Leu Leu Gln Leu Arg Leu Val Ser 245 250 255Ser Asp Asp Glu Ser Leu Val Ala Met Glu Gly Ala Thr Val Asp Glu 260 265 270Asp His His Leu Phe Thr Thr Lys Lys Gly Lys Ser Ser Leu Ser Phe 275 280 285Asp Leu Asp Ile 290217420PRTArtificial Sequencealignment 217Met Glu Leu Met Gln Gln Val Lys Gly Asn Tyr Ser Asp Ser Arg Glu1 5 10 15Glu Glu Glu Glu Glu Glu Ala Ala Ala Ile Thr Arg Glu Ser Glu Ser 20 25 30Ser Arg Leu His Gln Gln Asp Thr Ala Ser Asn Phe Gly Lys Lys Leu 35 40 45Asp Leu Met Asp Leu Ser Leu Gly Ser Ser Lys Glu Glu Glu Glu Glu 50 55 60Gly Asn Leu Gln Gln Gly Gly Gly Gly Val Val His His Ala His Gln65 70 75 80Val Val Glu Lys Glu His Met Phe Glu Lys Val Ala Thr Pro Ser Asp 85 90 95Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys 100 105 110Tyr Phe Pro Leu Asp Ser Ser Thr Asn Glu Lys Gly Leu Leu Leu Asn 115 120 125Phe Glu Asp Arg Asn Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp 130 135 140Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val145 150 155 160Lys Glu Lys Lys Leu Asp Ala Gly Asp Ile Val Ser Phe Gln Arg Gly 165 170 175Leu Gly Asp Leu Tyr Arg His Arg Leu Tyr Ile Asp Trp Lys Arg Arg 180 185 190Pro Asp His Ala His Ala His Pro Pro His His His Asp Pro Leu Phe 195 200 205Leu Pro Ser Ile Arg Leu Tyr Ser Leu Pro Pro Thr Met Pro Pro Arg 210 215 220Tyr His His Asp His His Phe His His His Leu Asn Tyr Asn Asn Leu225 230 235 240Phe Thr Phe Gln Gln His Gln Tyr Gln Gln Leu Gly Ala Ala Thr Thr 245 250 255Thr His His Asn Asn Tyr Gly Tyr Gln Asn Ser Gly Ser Gly Ser Leu 260 265 270Tyr Tyr Leu Arg Ser Ser Met Ser Met Gly Gly Gly Asp Gln Asn Leu 275 280 285Gln Gly Arg Gly Ser Asn Ile Val Pro Met Ile Ile Asp Ser Val Pro 290 295 300Val Asn Val Ala His His Asn Asn Asn Arg His Gly Asn Gly Gly Ile305 310 315 320Thr Ser Gly Gly Thr Asn Cys Ser Gly Lys Arg Leu Arg Leu Phe Gly 325 330 335Val Asn Met Glu Cys Ala Ser Ser Ala Glu Asp Ser Lys Glu Leu Ser 340 345 350Ser Gly Ser Ala Ala His Val Thr Thr Ala Ala Ser Ser Ser Ser Leu 355 360 365His His Gln Arg Leu Arg Val Pro Val Pro Val Pro Leu Glu Asp Pro 370 375 380Leu Ser Ser Ser Ala Ala Ala Ala Ala Arg Phe Gly Asp His Lys Gly385 390 395 400Ala Ser Thr Gly Thr Ser Leu Leu Phe Asp Leu Asp Pro Ser Leu Gln 405 410 415Tyr His Arg His 420218422PRTArtificial Sequencealignment 218Met Asp Gln Phe Ala Ala Ser Gly Arg Phe Ser Arg Glu Glu Glu Ala1 5 10 15Asp Glu Glu Gln Glu Asp Ala Ser Asn Ser Met Arg Glu Ile Ser Phe 20 25 30Met Pro Pro Ala Ala Ala Ser Ser Ser Ser Ala Ala Ala Ser Ala Ser 35 40 45Ala Ser Ala Ser Thr Ser Ala Ser Ala Cys Ala Ser Gly Ser Ser Ser 50 55 60Ala Pro Phe Arg Ser Ala Ser Ala Ser Gly Asp Ala Ala Gly Ala Ser65 70 75 80Gly Ser Gly Gly Pro Ala Asp Ala Asp Ala Glu Ala Glu Ala Val Glu 85 90 95Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys 100 105 110Leu Asn Arg Leu Val Ile Pro Lys Gln Tyr Ala Glu Lys Tyr Phe Pro 115 120 125Leu Asp Ala Ala Ala Asn Glu Lys Gly Leu Leu Leu Ser Phe Glu Asp 130 135 140Ser Ala Gly Lys His Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser145 150 155 160Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 165 170 175Arg Leu Val Ala Gly Asp Thr Val Ser Phe Ser Arg Ala Ala Ala Glu 180 185 190Asp Ala Arg His Arg Leu Phe Ile Asp Trp Lys Arg Arg Val Asp Thr 195 200 205Arg Gly Pro Leu Arg Phe Ser Gly Leu Ala Leu Pro Met Pro Leu Pro 210 215 220Ser Ser His Tyr Gly Gly Pro His His Tyr Ser Pro Trp Gly Phe Gly225 230 235 240Gly Gly Gly Gly Gly Gly Gly Gly Phe Phe Met Pro Pro Ser Pro Pro 245 250 255Ala Thr Leu Tyr Glu His Arg Leu Arg Gln Gly Leu Asp Phe Arg Ser 260 265 270Met Thr Thr Thr Tyr Pro Ala Pro Thr Val Gly Arg Gln Leu Leu Phe 275 280 285Phe Gly Ser Ala Arg Met Pro Pro His His Ala Pro Pro Pro Gln Pro 290 295 300Arg Pro Phe Ser Leu Pro Leu His His Tyr Thr Val Gln Pro Ser Ala305 310 315 320Ala Gly Val Thr Ala Ala Ser Arg Pro Val Leu Leu Asp Ser Val Pro 325 330 335Val Ile Glu Ser Pro Thr Thr Ala Ala Lys Arg Val Arg Leu Phe Gly 340 345 350Val Asn Leu Asp Asn Asn Pro Asp Gly Gly Gly Glu Ala Ser His Gln 355 360 365Gly Asp Ala Leu Ser Leu Gln Met Pro Gly Trp Gln Gln Arg Thr Pro 370 375 380Thr Leu Arg Leu Leu Glu Leu Pro Arg His Gly Gly Glu Ser Ser Ala385 390 395 400Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser Lys Arg Glu Ala Arg Ser 405 410 415Ala Leu Asp Leu Asp Leu 420219412PRTArtificial Sequencealignment 219Met Glu Phe Thr Thr Ser Ser Arg Phe Ser Lys Glu Glu Glu Asp Glu1 5 10 15Glu Gln Asp Glu Ala Gly Arg Arg Glu Ile Pro Phe Met Thr Ala Thr 20 25 30Ala Glu Ala Ala Pro Ala Pro Thr Ser Ser Ser Ser Ser Pro Ala His 35 40 45His Ala Ala Ser Ala Ser Ala Ser Ala Ser Ala Ser Gly Ser Ser Thr 50 55 60Pro Phe Arg Ser Asp Asp Gly Ala Gly Ala Ser Gly Ser Gly Gly Gly65 70 75 80Gly Gly Gly Gly Gly Glu Ala Glu Val Val Glu Lys Glu His Met Phe 85 90 95Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val 100 105 110Ile Pro Lys Gln Tyr Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala Ala 115 120 125Asn Glu Lys Gly Leu Leu Leu Asn Phe Glu Asp Arg Ala Gly Lys Pro 130 135 140Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met145 150 155 160Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly 165 170 175Asp Thr Val Ser Phe Ser Arg Gly Ile Gly Asp Glu Ala Ala Arg His 180 185 190Arg Leu Phe Ile Asp Trp Lys Arg Arg Ala Asp Thr Arg Asp Pro Leu 195 200 205Arg Leu Pro Arg Gly Leu Pro Leu Pro Met Pro Leu Thr Ser His Tyr 210 215 220Ala Pro Trp Gly Ile Gly Gly Gly Gly Gly Phe Phe Val Gln Pro Ser225 230 235 240Pro Pro Ala Thr Leu Tyr Glu His Arg Leu Arg Gln Gly Leu Asp Phe 245 250 255Arg Ala Phe Asn Pro Ala Ala Ala Met Gly Arg Gln Val Leu Leu Phe 260 265 270Gly Ser Ala Arg Ile Pro Pro Gln Ala Pro Leu Leu Ala Arg Ala Pro 275 280 285Ser Pro Leu His His His Tyr Thr Leu Gln Pro Ser Gly Asp Gly Val 290 295 300Arg Ala Ala Gly Ser Pro Val Val Leu Asp Ser Val Pro Val Ile Glu305 310 315 320Ser Pro Thr Thr Ala Ala Lys Arg Val Arg Leu Phe Gly Val Asn Leu 325 330 335Asp Asn Pro His Ala Gly Gly Gly Gly Gly Ala Ala Ala Gly Glu Ser 340 345 350Ser Asn His Gly Asn Ala Leu Ser Leu Gln Thr Pro Ala Trp Met Arg 355 360 365Arg Asp Pro Thr Leu Arg Leu Leu Glu Leu Pro Pro His His His His 370 375 380Gly Ala Glu Ser Ser Ala Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser385 390 395 400Lys Arg Asp Ala His Ser Ala Leu Asp Leu Asp Leu 405 410220409PRTArtificial Sequencealignment 220Met Glu Phe Thr Ala Thr Ser Ser Arg Phe Ser Lys Gly Glu Glu Glu1 5 10 15Val Glu Glu Glu Gln Glu Glu Ala Ser Met Arg Glu Ile Pro Phe Met 20 25 30Thr Pro Ala Ala Ala Thr Cys Ala Ala Ala Pro Pro Ser Ala Ser Ala 35 40 45Ser Ala Ser Thr Pro Ala Ser Ala Ser Gly Ser Ser Pro Pro Phe Arg 50 55 60Ser Gly Asp Asp Ala Gly Ala Ser Gly Ser Gly Ala Gly Asp Gly Ser65 70 75 80Arg Ser Asn Val Ala Glu Ala Val Glu Lys Glu His Met Phe Asp Lys 85 90 95Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 100 105 110Lys Gln Tyr Ala Glu Lys Tyr Phe Pro Leu Asp Ser Ala Ala Asn Glu 115 120 125Lys Gly Leu Leu Leu Asn Phe Glu Asp Ser Ala Gly Lys Pro Trp Arg 130 135 140Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys145 150 155 160Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr 165 170 175Val Ser Phe Ser Arg Gly Ala Gly Glu Ala Ala Arg His Arg Leu Phe 180 185 190Ile Asp Trp Lys Arg Arg Ala Asp Thr Arg Asp Pro Leu Arg Leu Pro 195 200 205Arg Leu Pro Leu Pro Met Pro Leu Thr Ser His Tyr Ser Pro Trp Gly 210 215 220Leu Gly Ala Gly Ala Arg Gly Phe Phe Met Pro Pro Ser Pro Pro Ala225 230 235 240Thr Leu Tyr Glu His Arg Leu Arg Gln Gly Phe Asp Phe Arg Gly Met 245 250 255Asn Pro Ser Tyr Pro Thr Met Gly Arg Gln Val Ile Leu Phe Gly Ser 260 265 270Ala Ala Arg Met Pro Pro His Gly Pro Ala Pro Leu Leu Val Pro Arg 275 280 285Pro Pro Pro Pro Leu His Phe Thr Val Gln Gln Gln Gly Ser Asp Ala 290 295 300Gly Gly Ser Val Thr Ala Gly Ser Pro Val Val Leu Asp Ser Val Pro305 310 315 320Val Ile Glu Ser Pro Thr Thr Ala Thr Lys Lys Arg Val Arg Leu Phe 325 330 335Gly Val Asn Leu Asp Asn Pro Gln His Pro Gly Asp Gly Gly Gly Glu 340 345 350Ser Ser Asn Tyr Gly Ser Ala Leu Pro Leu Gln Met Pro Ala Ser Ala 355 360 365Trp Arg Pro Arg Asp His Thr Leu Arg Leu Leu Glu Phe Pro Ser His 370 375 380Gly Ala Glu Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser Lys Arg Glu385 390 395 400Ala His Ser Gly Leu Asp Leu Asp Leu 405221316PRTArtificial Sequencealignment 221Met Glu Phe Ala Thr Thr Ser Ser Arg Phe Ser Lys Glu Glu Glu Glu1 5 10 15Glu Glu Glu Gly Glu Gln Glu Met Glu Gln Glu Gln Asp Glu Glu Glu 20 25 30Glu Glu Ala Glu Ala Ser Pro Arg Glu Ile Pro Phe Met Thr Ser Ala 35 40 45Ala Ala Ala Ala Thr Ala Ser Ser Ser Ser Pro Thr Ser Val Ser Pro 50 55 60Ser Ala Thr Ala Ser Ala Ala Ala Ser Thr Ser Ala Ser Gly Ser Pro65 70 75 80Phe Arg Ser Ser Asp Gly Ala Gly Ala Ser Gly Ser Gly Gly Gly Gly 85 90 95Gly Gly Glu Asp Val Glu Val Ile Glu Lys Glu His Met Phe Asp Lys 100 105 110Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 115 120 125Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ser Ala Ala Asn Glu 130 135 140Lys Gly Leu Leu Leu Ser Phe Glu Asp Arg Thr Gly Lys Leu Trp Arg145 150 155 160Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys 165 170 175Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr 180 185 190Val Ser Phe Cys Arg Gly Ala Ala Glu Ala Thr Arg Asp Arg Leu Phe 195 200 205Ile Asp Trp Lys Arg Arg Ala Asp Val Arg Asp Pro His Arg Phe Gln 210 215 220Arg Leu Pro Leu Pro Met Thr Ser Pro Tyr Gly Pro Trp Gly Gly Gly225 230 235 240Ala Gly Ala Ser Ser Cys Arg Pro Arg Arg Pro Pro Arg Ser Thr Ser 245 250 255Ile Thr Ala Phe Ala Arg Ala Ser Thr Ser Ala Thr Ser Thr Pro Leu 260 265 270Cys Arg Arg Gly Ser Ser Ser Ser Ser Ala Pro Gln Gly Arg Gly Phe 275

280 285Ile Ser Thr Arg Pro Cys His Arg Arg Arg Arg His Leu Arg Leu Leu 290 295 300Thr Asn Ser Thr Leu Arg Cys Thr Thr Arg Ala Pro305 310 315222409PRTArtificial Sequencealignment 222Met Glu Phe Ala Ser Ser Ser Ser Arg Phe Ser Arg Glu Glu Asp Glu1 5 10 15Glu Glu Glu Gln Glu Glu Glu Glu Glu Glu Glu Glu Ala Ser Pro Arg 20 25 30Glu Ile Pro Phe Met Thr Ala Ala Ala Thr Ala Asp Thr Gly Ala Ala 35 40 45Ala Ser Ser Ser Ser Pro Ser Ala Ala Ala Ser Ser Gly Pro Ala Ala 50 55 60Ala Pro Arg Ser Ser Asp Gly Ala Gly Ala Ser Gly Ser Gly Gly Gly65 70 75 80Gly Ser Asp Asp Val Gln Val Ile Glu Lys Glu His Met Phe Asp Lys 85 90 95Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 100 105 110Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala Ala Asn Glu 115 120 125Lys Gly Gln Leu Leu Ser Phe Glu Asp Arg Ala Gly Lys Leu Trp Arg 130 135 140Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys145 150 155 160Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr 165 170 175Val Ser Phe Cys Arg Gly Ala Gly Asp Thr Ala Arg Asp Arg Leu Phe 180 185 190Ile Asp Trp Lys Arg Arg Ala Asp Ser Arg Asp Pro His Arg Met Pro 195 200 205Arg Leu Pro Leu Pro Met Ala Pro Val Ala Ser Pro Tyr Gly Pro Trp 210 215 220Gly Gly Gly Gly Gly Gly Gly Ala Gly Gly Phe Phe Met Pro Pro Ala225 230 235 240Pro Pro Ala Thr Leu Tyr Glu His His Arg Phe Arg Gln Ala Leu Asp 245 250 255Phe Arg Asn Ile Asn Ala Ala Ala Ala Pro Ala Arg Gln Leu Leu Phe 260 265 270Phe Gly Ser Ala Gly Met Pro Pro Arg Ala Ser Met Pro Gln Gln Gln 275 280 285Gln Pro Pro Pro Pro Pro His Pro Pro Leu His Ser Ile Met Leu Val 290 295 300Gln Pro Ser Pro Ala Pro Pro Thr Ala Ser Val Pro Met Leu Leu Asp305 310 315 320Ser Val Pro Leu Val Asn Ser Pro Thr Ala Ala Ser Lys Arg Val Arg 325 330 335Leu Phe Gly Val Asn Leu Asp Asn Pro Gln Pro Gly Thr Ser Ala Glu 340 345 350Ser Ser Gln Asp Ala Asn Ala Leu Ser Leu Arg Thr Pro Gly Trp Gln 355 360 365Arg Pro Gly Pro Leu Arg Phe Phe Glu Ser Pro Gln Arg Gly Ala Glu 370 375 380Ser Ser Ala Ala Ser Ser Pro Ser Ser Ser Ser Ser Ser Lys Arg Glu385 390 395 400Ala His Ser Ser Leu Asp Leu Asp Leu 405223312PRTArtificial Sequencealignment 223Met Glu Phe Thr Pro Ile Ser Pro Pro Thr Arg Val Ala Gly Gly Glu1 5 10 15Glu Asp Ser Glu Arg Gly Ala Ala Ala Trp Ala Val Val Glu Lys Glu 20 25 30His Met Phe Glu Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn 35 40 45Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Leu Asp 50 55 60Ala Ala Ala Gly Ala Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly Gly65 70 75 80Gly Gly Lys Gly Leu Val Leu Ser Phe Glu Asp Arg Thr Gly Lys Ala 85 90 95Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met 100 105 110Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Gly Ala Gly 115 120 125Asp Thr Val Ser Phe Gly Arg Gly Leu Gly Asp Ala Ala Arg Gly Arg 130 135 140Leu Phe Ile Asp Phe Arg Arg Arg Arg Gln Asp Ala Gly Ser Phe Met145 150 155 160Phe Pro Pro Thr Ala Ala Pro Pro Ser His Ser His His His His Gln 165 170 175Arg His His Pro Pro Leu Pro Ser Val Pro Leu Cys Pro Trp Arg Asp 180 185 190Tyr Thr Thr Ala Tyr Gly Gly Gly Tyr Gly Tyr Gly Tyr Gly Gly Gly 195 200 205Ser Thr Pro Ala Ser Ser Arg His Val Leu Phe Leu Arg Pro Gln Val 210 215 220Pro Ala Ala Val Val Leu Lys Ser Val Pro Val His Val Ala Ala Thr225 230 235 240Ser Ala Val Gln Glu Ala Ala Thr Thr Thr Arg Pro Lys Arg Val Arg 245 250 255Leu Phe Gly Val Asn Leu Asp Cys Pro Ala Ala Met Asp Asp Asp Asp 260 265 270Asp Ile Ala Gly Ala Ala Ser Arg Thr Ala Ala Ser Ser Leu Leu Gln 275 280 285Leu Pro Ser Pro Ser Ser Ser Thr Ser Ser Ser Thr Ala Gly Lys Lys 290 295 300Met Cys Ser Leu Asp Leu Gly Leu305 310224277PRTArtificial Sequencealignment 224Met Glu Phe Thr Pro Ala His Ala His Ala Arg Val Val Glu Asp Ser1 5 10 15Glu Arg Pro Arg Gly Gly Val Ala Trp Val Glu Lys Glu His Met Phe 20 25 30Glu Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val 35 40 45Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Ala Leu Asp Ala Ser 50 55 60Ser Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Gly Gly Gly Lys Gly65 70 75 80Leu Val Leu Ser Phe Glu Asp Arg Ala Gly Lys Ala Trp Arg Phe Arg 85 90 95Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp 100 105 110Ser Arg Phe Val Lys Glu Lys Arg Leu Gly Ala Gly Asp Thr Val Leu 115 120 125Phe Ala Arg Gly Ala Gly Gly Ala Arg Gly Arg Phe Phe Ile Asp Phe 130 135 140Arg Arg Arg Arg Gln Asp Leu Ala Phe Leu Gln Pro Thr Leu Ala Ser145 150 155 160Ala Gln Arg Leu Leu Pro Leu Pro Ser Val Pro Ile Cys Pro Trp Gln 165 170 175Asp Tyr Gly Ala Ser Ala Pro Ala Pro Asn Arg His Val Leu Phe Leu 180 185 190Arg Pro Gln Val Pro Ala Ala Val Val Leu Lys Ser Val Pro Val His 195 200 205Val Ala Ala Ser Ala Val Glu Ala Thr Met Ser Lys Arg Val Arg Leu 210 215 220Phe Gly Val Asn Leu Asp Cys Pro Pro Asp Ala Glu Asp Ser Ala Thr225 230 235 240Val Pro Arg Gly Arg Ala Ala Ser Thr Thr Leu Leu Gln Leu Pro Ser 245 250 255Pro Ser Ser Ser Thr Ser Ser Ser Thr Ala Gly Lys Asp Val Cys Cys 260 265 270Leu Asp Leu Gly Leu 275225273PRTArtificial Sequencealignment 225Met Glu Phe Arg Pro Ala His Ala Arg Val Phe Glu Asp Ser Glu Arg1 5 10 15Pro Arg Gly Gly Val Ala Trp Leu Glu Lys Glu His Met Phe Glu Lys 20 25 30Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 35 40 45Lys Gln His Ala Glu Arg Tyr Phe Pro Ala Leu Asp Ala Ser Ala Ala 50 55 60Ala Ala Ser Ala Ser Ala Ser Ala Gly Gly Gly Lys Ala Gly Leu Val65 70 75 80Leu Ser Phe Glu Asp Arg Ala Gly Lys Ala Trp Arg Phe Arg Tyr Ser 85 90 95Tyr Trp Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg 100 105 110Phe Val Lys Glu Lys Arg Leu Gly Ala Gly Asp Thr Val Leu Phe Ala 115 120 125Arg Gly Ala Gly Ala Thr Arg Gly Arg Phe Phe Ile Asp Phe Arg Arg 130 135 140Arg Arg His Glu Leu Ala Phe Leu Gln Pro Pro Leu Ala Ser Ala Gln145 150 155 160Arg Leu Leu Pro Leu Pro Ser Val Pro Ile Cys Pro Trp Gln Gly Tyr 165 170 175Gly Ala Ser Ala Pro Ala Pro Ser Arg His Val Leu Phe Leu Arg Pro 180 185 190Gln Val Pro Ala Ala Val Val Leu Thr Ser Val Pro Val Arg Val Ala 195 200 205Ala Ser Ala Val Glu Glu Ala Thr Arg Ser Lys Arg Val Arg Leu Phe 210 215 220Gly Val Asn Leu Asp Cys Pro Pro Asp Ala Glu Asp Gly Ala Thr Ala225 230 235 240Thr Arg Thr Pro Ser Thr Leu Leu Gln Leu Pro Ser Pro Ser Ser Ser 245 250 255Thr Ser Ser Ser Thr Gly Gly Lys Asp Val Arg Ser Leu Asp Leu Gly 260 265 270Leu226282PRTArtificial Sequencealignment 226Met Glu Phe Ile Thr Pro Ile Val Arg Pro Ala Ser Ala Ala Ala Gly1 5 10 15Gly Gly Glu Val Gln Glu Ser Glu Arg Pro Arg Gly Gly Val Ala Trp 20 25 30Leu Glu Lys Glu His Met Phe Glu Lys Val Val Thr Pro Ser Asp Val 35 40 45Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr 50 55 60Phe Pro Ala Leu Asp Ala Ser Ala Ala Ala Ala Ser Ala Ser Ala Ser65 70 75 80Ala Gly Gly Gly Lys Ala Gly Leu Val Leu Ser Phe Glu Asp Arg Ala 85 90 95Gly Lys Ala Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser 100 105 110Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu 115 120 125Gly Ala Gly Asp Thr Val Leu Phe Ala Arg Gly Ala Gly Ala Thr Arg 130 135 140Gly Arg Phe Phe Ile Asp Phe Arg Arg Arg Arg His Glu Leu Ala Phe145 150 155 160Leu Gln Pro Pro Leu Ala Ser Ala Gln Arg Leu Leu Pro Leu Pro Ser 165 170 175Val Pro Ile Cys Pro Trp Gln Gly Tyr Gly Ala Ser Ala Pro Ala Pro 180 185 190Ser Arg His Val Leu Phe Leu Arg Pro Gln Val Pro Ala Ala Val Val 195 200 205Leu Thr Ser Val Pro Val Arg Val Ala Ala Ser Ala Val Glu Glu Ala 210 215 220Thr Arg Ser Lys Arg Val Arg Leu Phe Gly Val Asn Leu Asp Cys Pro225 230 235 240Pro Asp Ala Glu Asp Gly Ala Thr Ala Thr Arg Thr Pro Ser Thr Leu 245 250 255Leu Gln Leu Pro Ser Pro Ser Ser Ser Thr Ser Ser Ser Thr Gly Gly 260 265 270Lys Asp Val Arg Ser Leu Asp Leu Gly Leu 275 280227259PRTArtificial Sequencealignment 227Met Glu Phe Thr Thr Pro Pro Pro Ala Thr Arg Ser Gly Gly Gly Glu1 5 10 15Glu Arg Ala Ala Ala Glu His Asn Gln His His Gln Gln Gln His Ala 20 25 30Thr Val Glu Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp 35 40 45Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys 50 55 60Tyr Phe Pro Leu Asp Ala Ala Ala Asn Glu Lys Gly Leu Leu Leu Ser65 70 75 80Phe Glu Asp Arg Thr Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val 100 105 110Lys Glu Lys Arg Leu Asp Ala Gly Asp Thr Val Ser Phe Gly Arg Gly 115 120 125Ile Ser Glu Ala Ala Arg Asp Arg Leu Phe Ile Asp Trp Arg Cys Arg 130 135 140Pro Asp Pro Pro Val Val His His Gln Tyr His His Arg Leu Pro Leu145 150 155 160Pro Ser Ala Val Val Pro Tyr Ala Pro Trp Ala Ala His Ala His His 165 170 175His His Tyr Pro Ala Asp Gly His Thr Glu Pro Val Thr Pro Cys Leu 180 185 190Cys Ala Thr Leu Val Ala Thr Glu Met Arg Ala Ser Ser Ser Gln Leu 195 200 205Ser Leu Thr Arg Ser Asn Leu Ser Arg Pro Pro Gln Pro Arg Ile Ala 210 215 220Arg Val Asp Gly Ala Gln Pro Arg Pro Ser Ser Ser Pro Arg Gln Pro225 230 235 240Gln Ser Leu Trp Cys Arg Ser Cys Gln Pro Gln Pro Arg Arg Thr Ala 245 250 255Asp Val Pro228327PRTArtificial Sequencealignment 228Met Glu Phe Thr Ala Pro Pro Pro Ala Thr Arg Ser Gly Gly Gly Glu1 5 10 15Glu Arg Ala Ala Ala Glu His His Gln Gln Gln Gln Gln Ala Thr Val 20 25 30Glu Lys Glu His Met Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly 35 40 45Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 50 55 60Pro Leu Asp Ala Ala Ala Asn Asp Lys Gly Leu Leu Leu Ser Phe Glu65 70 75 80Asp Arg Ala Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser 85 90 95Ser Gln Ser Tyr Val Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu 100 105 110Lys Arg Leu Asp Ala Gly Asp Thr Val Ser Phe Gly Arg Gly Val Gly 115 120 125Glu Ala Ala Arg Gly Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Asp 130 135 140Pro Pro Val Val His His Gln Tyr His His His Arg Leu Pro Leu Pro145 150 155 160Ser Ala Val Val Pro Tyr Ala Pro Trp Ala Ala Ala Ala His Ala His 165 170 175His His His Tyr Pro Ala Ala Gly Val Gly Ala Ala Arg Thr Thr Thr 180 185 190Thr Thr Thr Thr Thr Val Leu His His Leu Pro Pro Ser Pro Ser Pro 195 200 205Leu Tyr Leu Asp Thr Arg Arg Arg His Val Gly Tyr Asp Ala Tyr Gly 210 215 220Ala Gly Thr Arg Gln Leu Leu Phe Tyr Arg Pro His Gln Gln Pro Ser225 230 235 240Thr Thr Val Met Leu Asp Ser Val Pro Val Arg Leu Pro Pro Thr Pro 245 250 255Gly Gln His Ala Glu Pro Pro Pro Pro Ala Val Ala Ser Ser Ala Ser 260 265 270Lys Arg Val Arg Leu Phe Gly Val Asn Leu Asp Cys Ala Ala Ala Ala 275 280 285Gly Ser Glu Glu Glu Asn Val Gly Gly Trp Arg Thr Ser Ala Pro Pro 290 295 300Thr Gln Gln Ala Ser Ser Ser Ser Ser Tyr Ser Ser Gly Lys Ala Arg305 310 315 320Cys Ser Leu Asn Leu Asp Leu 325229279PRTArtificial Sequencealignment 229Met Ala Met Asn His Pro Leu Phe Ser Gln Glu Gln Pro Gln Ser Trp1 5 10 15Pro Trp Gly Val Ala Met Tyr Ala Asn Phe His Tyr His His His Tyr 20 25 30Glu Lys Glu His Met Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly 35 40 45Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 50 55 60Pro Leu Gly Ala Gly Asp Ala Ala Asp Lys Gly Leu Ile Leu Ser Phe65 70 75 80Glu Asp Glu Ala Gly Ala Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Thr 85 90 95Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys 100 105 110Glu Lys Arg Leu Asp Ala Gly Asp Val Val His Phe Glu Arg Val Arg 115 120 125Gly Ser Phe Gly Val Gly Asp Arg Leu Phe Ile Gly Cys Arg Arg Arg 130 135 140Gly Asp Ala Ala Ala Ala Gln Thr Pro Ala Pro Pro Pro Ala Val Arg145 150 155 160Val Ala Pro Ala Ala Gln Asn Ala Gly Glu Gln Gln Pro Trp Ser Pro 165 170 175Met Cys Tyr Ser Thr Ser Gly Gly Gly Ser Tyr Pro Thr Ser Pro Ala 180 185 190Asn Ser Tyr Ala Tyr Arg Arg Ala Ala Asp His Asp His Gly Asp Met 195 200 205His His Ala Asp Glu Ser Pro Arg Asp Thr Asp Ser Pro Ser Phe Ser 210 215 220Ala Gly Ser Ala Pro Ser Arg Arg Leu Arg Leu Phe Gly Val Asn Leu225 230 235 240Asp Cys Gly Pro Glu Pro Glu Ala Asp Thr Thr Ala Ala Ala Thr Met 245 250 255Tyr Gly Tyr Met His Gln Gln Ser Ser Tyr Ala Ala Met Ser Ala Val

260 265 270Pro Ser Tyr Trp Gly Asn Ser 275230307PRTArtificial Sequencealignment 230Met Ala Thr Asn His Leu Ser Gln Gly Gln His Gln His Pro Gln Ala1 5 10 15Trp Pro Trp Gly Val Ala Met Tyr Thr Asn Leu His Tyr His His Gln 20 25 30Gln His His His Tyr Glu Lys Glu His Leu Phe Glu Lys Pro Leu Thr 35 40 45Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His 50 55 60Ala Glu Arg Tyr Phe Pro Leu Ser Ser Ser Gly Ala Gly Asp Lys Gly65 70 75 80Leu Ile Leu Cys Phe Glu Asp Asp Asp Asp Asp Glu Ala Ala Ala Ala 85 90 95Asn Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Thr Ser Ser Gln Ser 100 105 110Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Glu Lys Gln Leu 115 120 125Asp Ala Gly Asp Val Val Arg Phe Gln Arg Met Arg Gly Phe Gly Met 130 135 140Pro Asp Arg Leu Phe Ile Ser His Ser Arg Arg Gly Glu Thr Thr Ala145 150 155 160Thr Ala Ala Thr Thr Val Pro Pro Ala Ala Ala Ala Val Arg Val Val 165 170 175Val Ala Pro Ala Gln Ser Ala Gly Ala Asp His Gln Gln Gln Gln Gln 180 185 190Pro Ser Pro Trp Ser Pro Met Cys Tyr Ser Thr Ser Gly Ser Tyr Ser 195 200 205Tyr Pro Thr Ser Ser Pro Ala Asn Ser Gln His Ala Tyr His Arg His 210 215 220Ser Ala Asp His Asp His Ser Asn Asn Met Gln His Ala Gly Glu Ser225 230 235 240Gln Ser Asp Arg Asp Asn Arg Ser Cys Ser Ala Ala Ser Ala Pro Pro 245 250 255Pro Pro Ser Arg Arg Leu Arg Leu Phe Gly Val Asn Leu Asp Cys Gly 260 265 270Pro Gly Pro Glu Pro Glu Thr Pro Thr Ala Met Tyr Gly Tyr Met His 275 280 285Gln Ser Pro Tyr Ala Tyr Asn Asn Trp Gly Ser Pro Tyr Gln His Asp 290 295 300Glu Glu Ile305231288PRTArtificial Sequencealignment 231Met Ser Ser Ile Asn His Tyr Ser Pro Glu Thr Thr Leu Tyr Trp Thr1 5 10 15Asn Asp Gln Gln Gln Gln Ala Ala Met Trp Leu Ser Asn Ser His Thr 20 25 30Pro Arg Phe Asn Leu Asn Asp Glu Glu Glu Glu Glu Glu Asp Asp Val 35 40 45Ile Val Ser Asp Lys Ala Thr Asn Asn Leu Thr Gln Glu Glu Glu Lys 50 55 60Val Ala Met Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys Leu65 70 75 80Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu 85 90 95Asp Ser Ser Ala Ala Lys Gly Leu Leu Leu Ser Phe Glu Asp Glu Ser 100 105 110Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser 115 120 125Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys Arg Leu 130 135 140His Ala Gly Asp Val Val Leu Phe His Arg His Arg Ser Leu Pro Gln145 150 155 160Arg Phe Phe Ile Ser Cys Ser Arg Arg Gln Pro Asn Pro Val Pro Ala 165 170 175His Val Ser Thr Thr Arg Ser Ser Ala Ser Phe Tyr Ser Ala His Pro 180 185 190Pro Tyr Pro Ala His His Phe Pro Phe Pro Tyr Gln Pro His Ser Leu 195 200 205His Ala Pro Gly Gly Gly Ser Gln Gly Gln Asn Glu Thr Thr Pro Gly 210 215 220Gly Asn Ser Ser Ser Ser Gly Ser Gly Arg Val Leu Arg Leu Phe Gly225 230 235 240Val Asn Met Glu Cys Gln Pro Asp Asn His Asn Asp Ser Gln Asn Ser 245 250 255Thr Pro Glu Cys Ser Tyr Thr His Leu Tyr His His Gln Thr Ser Ser 260 265 270Tyr Ser Ser Ser Ser Asn Pro His His His Met Val Pro Gln Gln Pro 275 280 285232337PRTArtificial Sequencealignment 232Met Ser Ile Asn His Tyr Ser Met Asp Leu Pro Glu Pro Thr Leu Trp1 5 10 15Trp Pro His Pro His His Gln Gln Gln Gln Leu Thr Leu Met Asp Pro 20 25 30Asp Pro Leu Arg Leu Asn Leu Asn Ser Asp Asp Gly Asn Gly Asn Asp 35 40 45Asn Asp Asn Asp Glu Asn Gln Thr Thr Thr Thr Gly Gly Glu Gln Glu 50 55 60Ile Leu Asp Asp Lys Glu Pro Met Phe Glu Lys Pro Leu Thr Pro Ser65 70 75 80Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu 85 90 95Lys Tyr Phe Pro Leu Ser Gly Asp Ser Gly Gly Ser Glu Cys Lys Gly 100 105 110Leu Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys Cys Trp Arg Phe Arg 115 120 125Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp 130 135 140Ser Arg Tyr Val Lys Asp Lys Arg Leu Asp Ala Gly Asp Val Val Leu145 150 155 160Phe Glu Arg His Arg Val Asp Ala Gln Arg Leu Phe Ile Gly Trp Arg 165 170 175Arg Arg Arg Gln Ser Asp Ala Ala Leu Pro Pro Ala His Val Ser Ser 180 185 190Arg Lys Ser Gly Gly Gly Asp Gly Asn Ser Asn Lys Asn Glu Gly Trp 195 200 205Thr Arg Gly Phe Tyr Ser Ala His His Pro Tyr Pro Thr His His Leu 210 215 220His His His Gln Pro Ser Pro Tyr Gln Gln Gln His Asp Cys Leu His225 230 235 240Ala Gly Arg Gly Ser Gln Gly Gln Asn Gln Arg Met Arg Pro Val Gly 245 250 255Asn Asn Ser Ser Ser Ser Ser Ser Ser Ser Arg Val Leu Arg Leu Phe 260 265 270Gly Val Asp Met Glu Cys Gln Pro Glu His Asp Asp Ser Gly Pro Ser 275 280 285Thr Pro Gln Cys Ser Tyr Asn Ser Asn Asn Met Leu Pro Ser Thr Gln 290 295 300Gly Thr Asp His Ser His His Asn Phe Tyr Gln Gln Gln Pro Ser Asn305 310 315 320Ser Asn Pro Ser Pro His His Met Met Val His His Gln Pro Tyr Tyr 325 330 335Tyr233344PRTArtificial Sequencealignment 233Met Ser Thr Asn His Tyr Thr Met Asp Leu Pro Glu Pro Thr Leu Trp1 5 10 15Trp Pro His Pro His Gln Gln Gln Leu Thr Leu Ile Asp Pro Asp Pro 20 25 30Leu Pro Leu Asn Leu Asn Asn Asp Asp Asn Asp Asn Gly Asp Asp Asn 35 40 45Asp Asn Asp Glu Asn Gln Thr Val Thr Thr Thr Thr Thr Gly Gly Glu 50 55 60Glu Glu Ile Ile Asn Asn Lys Glu Pro Met Phe Glu Lys Pro Leu Thr65 70 75 80Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His 85 90 95Ala Glu Lys Tyr Phe Pro Leu Ser Gly Gly Asp Ser Gly Ser Ser Glu 100 105 110Cys Lys Gly Leu Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys Cys Trp 115 120 125Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr 130 135 140Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys Arg Leu Asp Ala Gly Asp145 150 155 160Val Val Leu Phe Gln Arg His Arg Ala Asp Ala Gln Arg Leu Phe Ile 165 170 175Gly Trp Arg Arg Arg Arg Gln Ser Asp Ala Leu Pro Pro Pro Ala His 180 185 190Val Ser Ser Arg Lys Ser Gly Gly Asp Gly Asn Ser Ser Lys Asn Glu 195 200 205Gly Asp Val Gly Val Gly Trp Thr Arg Gly Phe Tyr Pro Ala His His 210 215 220Pro Tyr Pro Thr His His His His Pro Ser Pro Tyr His His Gln Gln225 230 235 240Asp Asp Ser Leu His Ala Val Arg Gly Ser Gln Gly Gln Asn Gln Arg 245 250 255Thr Arg Pro Val Gly Asn Ser Ser Ser Ser Ser Ser Ser Ser Ser Arg 260 265 270Val Leu Arg Leu Phe Gly Val Asn Met Glu Cys Gln Pro Glu His Asp 275 280 285Asp Ser Gly Pro Ser Thr Pro Gln Cys Ser Tyr Asn Thr Asn Asn Ile 290 295 300Leu Pro Ser Thr Gln Gly Thr Asp Ile His Ser His Leu Asn Phe Tyr305 310 315 320Gln Gln Gln Gln Thr Ser Asn Ser Lys Pro Pro Pro His His Met Met 325 330 335Ile Arg His Gln Pro Tyr Tyr Tyr 340234245PRTArtificial Sequencealignment 234Met Ser Ile Asn Gln Tyr Ser Ser Glu Phe Tyr Tyr His Ser Leu Met1 5 10 15Trp Gln Gln Gln Gln Gln His His His Gln Asn Glu Val Val Glu Glu 20 25 30Lys Glu Ala Leu Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys 35 40 45Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro 50 55 60Leu Ala Ala Ala Ala Val Asp Ala Val Glu Lys Gly Leu Leu Leu Cys65 70 75 80Phe Glu Asp Glu Glu Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val 100 105 110Lys Glu Lys Gln Leu Asp Ala Gly Asp Val Val Leu Phe His Arg His 115 120 125Arg Ala Asp Gly Gly Arg Phe Phe Ile Gly Trp Arg Arg Arg Gly Asp 130 135 140Ser Ser Ser Ser Ser Asp Ser Tyr Arg Asn Leu Gln Ser Asn Ser Ser145 150 155 160Leu Gln Tyr Tyr Pro His Ala Gly Ala Gln Ala Val Glu Asn Gln Arg 165 170 175Gly Asn Ser Lys Thr Leu Arg Leu Phe Gly Val Asn Met Glu Cys Gln 180 185 190Ile Asp Ser Asp Trp Ser Glu Pro Ser Thr Pro Asp Gly Phe Thr Thr 195 200 205Cys Pro Thr Asn His Asp Gln Phe Pro Ile Tyr Pro Glu His Phe Pro 210 215 220Pro Pro Tyr Tyr Met Asp Val Ser Phe Thr Gly Asp Val His Gln Thr225 230 235 240Ser Ser Gln Gln Gly 245235244PRTArtificial Sequencealignment 235Met Ser Ile Asn Gln Tyr Ser Ser Asp Phe His Tyr His Ser Leu Met1 5 10 15Trp Gln Gln Gln Gln Gln Gln Gln Gln His Gln Asn Asp Val Val Glu 20 25 30Glu Lys Glu Ala Leu Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly 35 40 45Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe 50 55 60Pro Leu Ala Ala Ala Ala Ala Asp Ala Val Glu Lys Gly Leu Leu Leu65 70 75 80Cys Phe Glu Asp Glu Glu Gly Lys Pro Trp Arg Phe Arg Tyr Ser Tyr 85 90 95Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr 100 105 110Val Lys Glu Lys His Leu Asp Ala Gly Asp Val Val Leu Phe His Arg 115 120 125His Arg Ser Asp Gly Gly Arg Phe Phe Ile Gly Trp Arg Arg Arg Gly 130 135 140Asp Ser Ser Ser Ser Ser Asp Ser Tyr Arg His Val Gln Ser Asn Ala145 150 155 160Ser Leu Gln Tyr Tyr Pro His Ala Gly Ala Gln Ala Val Glu Ser Gln 165 170 175Arg Gly Asn Ser Lys Thr Leu Arg Leu Phe Gly Val Asn Met Glu Cys 180 185 190Gln Leu Asp Ser Asp Trp Ser Glu Pro Ser Thr Pro Asp Gly Ser Asn 195 200 205Thr Tyr Thr Thr Asn His Asp Gln Phe His Phe Tyr Pro Gln Gln Gln 210 215 220His Tyr Pro Pro Pro Tyr Tyr Met Asp Ile Ser Phe Thr Gly Asp Met225 230 235 240Asn Arg Thr Ser236248PRTArtificial Sequencealignment 236Met Ser Ile Asn Gln Tyr Ser Ser Asp Phe Asn Tyr His Ser Leu Met1 5 10 15Trp Gln Gln Gln Gln His Arg His His His His Gln Asn Asp Val Ala 20 25 30Glu Glu Lys Glu Ala Leu Phe Glu Lys Pro Leu Thr Pro Ser Asp Val 35 40 45Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg Tyr 50 55 60Phe Pro Leu Ala Ala Ala Ala Ala Asp Ala Met Glu Lys Gly Leu Leu65 70 75 80Leu Cys Phe Glu Asp Glu Glu Gly Lys Pro Trp Arg Phe Arg Tyr Ser 85 90 95Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg 100 105 110Tyr Val Lys Glu Lys Gln Leu Asp Ala Gly Asp Val Ile Leu Phe His 115 120 125Arg His Arg Val Asp Gly Gly Arg Phe Phe Ile Gly Trp Arg Arg Arg 130 135 140Gly Asn Ser Ser Ser Ser Ser Asp Ser Tyr Arg His Leu Gln Ser Asn145 150 155 160Ala Ser Leu Gln Tyr Tyr Pro His Ala Gly Val Gln Ala Val Glu Ser 165 170 175Gln Arg Gly Asn Ser Lys Thr Leu Arg Leu Phe Gly Val Asn Met Glu 180 185 190Cys Gln Leu Asp Ser Asp Leu Pro Asp Pro Ser Thr Pro Asp Gly Ser 195 200 205Thr Ile Cys Pro Thr Ser His Asp Gln Phe His Leu Tyr Pro Gln Gln 210 215 220His Tyr Pro Pro Pro Tyr Tyr Met Asp Ile Ser Phe Thr Gly Asp Val225 230 235 240His Gln Thr Arg Ser Pro Gln Gly 245237267PRTArtificial Sequencealignment 237Met Ser Val Asn His Tyr His Asn Thr Leu Ser Leu His His His His1 5 10 15Gln Asn Asp Val Ala Ile Ala Gln Arg Glu Ser Leu Phe Glu Lys Ser 20 25 30Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys 35 40 45Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Asn Asn Asn Asn Asn Gly 50 55 60Gly Ser Gly Asp Asp Val Ala Thr Thr Glu Lys Gly Met Leu Leu Ser65 70 75 80Phe Glu Asp Glu Ser Gly Lys Cys Trp Lys Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val 100 105 110Lys Asp Lys His Leu Asp Ala Gly Asp Val Val Phe Phe Gln Arg His 115 120 125Arg Phe Asp Leu His Arg Leu Phe Ile Gly Trp Arg Arg Arg Gly Glu 130 135 140Ala Ser Ser Ser Pro Ala Val Ser Val Val Ser Gln Glu Ala Leu Val145 150 155 160Asn Thr Thr Ala Tyr Trp Ser Gly Leu Thr Thr Pro Tyr Arg Gln Val 165 170 175His Ala Ser Thr Thr Tyr Pro Asn Ile His Gln Glu Tyr Ser His Tyr 180 185 190Gly Ala Val Val Asp His Ala Gln Ser Ile Pro Pro Val Val Ala Gly 195 200 205Ser Ser Arg Thr Val Arg Leu Phe Gly Val Asn Leu Glu Cys His Gly 210 215 220Asp Ala Val Glu Pro Pro Pro Arg Pro Asp Val Tyr Asn Asp Gln His225 230 235 240Ile Tyr Tyr Tyr Ser Thr Pro His Pro Met Asn Ile Ser Phe Ala Gly 245 250 255Glu Ala Leu Glu Gln Val Gly Asp Gly Arg Gly 260 265238264PRTArtificial Sequencealignment 238Met Ser Gly Asn His Tyr Ser Arg Asp Ile His His Asn Thr Pro Ser1 5 10 15Val His His His Gln Asn Tyr Ala Val Val Asp Arg Glu Tyr Leu Phe 20 25 30Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val 35 40 45Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Asn Asn Ala Gly 50 55 60Asp Asp Val Ala Ala Ala Glu Thr Thr Glu Lys Gly Met Leu Leu Thr65 70 75 80Phe Glu Asp Glu Ser Gly Lys Cys Trp Lys Phe Arg Tyr Ser Tyr Trp 85 90 95Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val 100 105 110Lys Asp Lys His Leu His Ala Gly Asp Val Val Phe Phe Gln Arg His 115 120 125Arg Phe Asp Leu His Arg Val Phe Ile Gly Trp Arg Lys Arg Gly Glu 130 135

140Val Ser Ser Pro Thr Ala Val Ser Val Val Ser Gln Glu Ala Arg Val145 150 155 160Asn Thr Thr Ala Tyr Trp Ser Gly Leu Thr Thr Pro Tyr Arg Gln Val 165 170 175His Ala Ser Thr Ser Ser Tyr Pro Asn Ile His Gln Glu Tyr Ser His 180 185 190Tyr Gly Ala Val Ala Glu Ile Pro Thr Val Val Thr Gly Ser Ser Arg 195 200 205Thr Val Arg Leu Phe Gly Val Asn Leu Glu Cys His Gly Asp Val Val 210 215 220Glu Thr Pro Pro Cys Pro Asp Gly Tyr Asn Gly Gln His Phe Tyr Tyr225 230 235 240Tyr Ser Thr Pro Asp Pro Met Asn Ile Ser Phe Ala Gly Glu Ala Met 245 250 255Glu Gln Val Gly Asp Gly Arg Arg 260239258PRTArtificial Sequencealignment 239Met Ser Val Asn His Tyr Ser Asn Thr Leu Ser Ser His Asn His His1 5 10 15Asn Glu His Lys Glu Ser Leu Phe Glu Lys Ser Leu Thr Pro Ser Asp 20 25 30Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Arg 35 40 45Tyr Leu Pro Leu Asn Asn Cys Gly Gly Gly Gly Asp Val Thr Ala Glu 50 55 60Ser Thr Glu Lys Gly Val Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys65 70 75 80Ser Trp Lys Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 85 90 95Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys His Leu Asn Ala 100 105 110Gly Asp Val Val Leu Phe Gln Arg His Arg Phe Asp Ile His Arg Leu 115 120 125Phe Ile Gly Trp Arg Arg Arg Gly Glu Ala Ser Ser Ser Ser Ala Val 130 135 140Ser Ala Val Thr Gln Asp Pro Arg Ala Asn Thr Thr Ala Tyr Trp Asn145 150 155 160Gly Leu Thr Thr Pro Tyr Arg Gln Val His Ala Ser Thr Ser Ser Tyr 165 170 175Pro Asn Asn Ile His Gln Glu Tyr Ser His Tyr Gly Pro Val Ala Glu 180 185 190Thr Pro Thr Val Ala Ala Gly Ser Ser Lys Thr Val Arg Leu Phe Gly 195 200 205Val Asn Leu Glu Cys His Ser Asp Val Val Glu Pro Pro Pro Cys Pro 210 215 220Asp Ala Tyr Asn Gly Gln His Ile Tyr Tyr Tyr Ser Thr Pro His Pro225 230 235 240Met Asn Ile Ser Phe Ala Gly Glu Ala Met Glu Gln Val Gly Asp Gly 245 250 255Arg Gly240278PRTArtificial Sequencealignment 240Met Ser Val Asn His Tyr Ser Thr Asp His His His Thr Leu Leu Trp1 5 10 15Gln Gln Gln Gln His Arg His Thr Thr Asp Thr Ser Glu Thr Thr Thr 20 25 30Thr Ala Thr Trp Leu His Asp Asp Leu Lys Glu Ser Leu Phe Glu Lys 35 40 45Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro 50 55 60Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Ala Val Leu Val Ser65 70 75 80Ser Ala Ala Ala Asp Thr Ser Ser Ser Leu Leu Ser Phe Glu Asp Glu 85 90 95Ser Gly Lys Ser Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln 100 105 110Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Asp Lys Gln 115 120 125Leu Asp Pro Gly Asp Val Val Phe Phe Gln Arg His Arg Ser Asp Ser 130 135 140Arg Arg Leu Phe Ile Gly Trp Arg Arg Arg Gly Gln Gly Ser Ser Ser145 150 155 160Ser Val Ala Ala Thr Asn Ser Ala Val Asn Thr Ser Ser Met Gly Ala 165 170 175Leu Ser Tyr His Gln Ile His Ala Thr Ser Asn Tyr Ser Asn Pro Pro 180 185 190Ser His Ser Glu Tyr Ser His Tyr Gly Ala Ala Val Ala Thr Ala Ala 195 200 205Glu Thr His Ser Thr Pro Ser Ser Ser Val Val Gly Ser Ser Arg Thr 210 215 220Val Arg Leu Phe Gly Val Asn Leu Glu Cys Gln Met Asp Glu Asn Asp225 230 235 240Gly Asp Asp Ser Val Ala Val Ala Thr Thr Val Glu Ser Pro Asp Gly 245 250 255Tyr Tyr Gly Gln Asn Met Tyr Tyr Tyr Tyr Ser His Pro His Asn Met 260 265 270Val Ile Leu Thr Leu Leu 275241267PRTArtificial Sequencealignment 241Met Ser Val Asn His Tyr Ser Thr Asp His His Gln Val His His His1 5 10 15His Thr Leu Phe Leu Gln Asn Leu His Thr Thr Asp Thr Ser Glu Pro 20 25 30Thr Thr Thr Ala Ala Thr Ser Leu Arg Glu Asp Gln Lys Glu Tyr Leu 35 40 45Phe Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu 50 55 60Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Thr Ile65 70 75 80Ile Ser Asn Asn Ala Glu Glu Lys Gly Met Leu Leu Ser Phe Glu Asp 85 90 95Glu Ser Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 100 105 110Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys 115 120 125Gln Leu Asp Pro Ala Asp Val Val Phe Phe Gln Arg Gln Arg Ser Asp 130 135 140Ser Arg Arg Leu Phe Ile Gly Trp Arg Arg Arg Gly Gln Gly Ser Ser145 150 155 160Ser Ala Ala Asn Thr Thr Ser Tyr Ser Ser Ser Met Thr Ala Pro Pro 165 170 175Tyr Ser Asn Tyr Ser Asn Arg Pro Ala His Ser Glu Tyr Ser His Tyr 180 185 190Gly Ala Ala Val Ala Thr Ala Thr Glu Thr His Phe Ile Pro Ser Ser 195 200 205Ser Ala Val Gly Ser Ser Arg Thr Val Arg Leu Phe Gly Val Asn Leu 210 215 220Glu Cys Gln Met Asp Glu Asp Glu Gly Asp Asp Ser Val Ala Thr Ala225 230 235 240Ala Ala Ala Glu Cys Pro Arg Gln Asp Ser Tyr Tyr Asp Gln Asn Met 245 250 255Tyr Asn Tyr Tyr Thr Pro His Ser Ser Ala Ser 260 265242347PRTArtificial Sequencealignment 242Met Ile Gly Val Glu Lys Val Thr Ile Cys Met Arg Ile Glu Val Asn1 5 10 15Thr Glu Lys Gly Arg Arg Ala Leu Met Asp Cys Trp Gln Ile Ser Gly 20 25 30Val His Glu Ser Ser Asp Cys Ser Glu Ile Lys Phe Ala Phe Asp Ala 35 40 45Val Val Lys Arg Ala Arg His Glu Glu Asn Asn Ala Ala Ala Gln Lys 50 55 60Phe Lys Gly Val Val Ser Gln Gln Asn Gly Asn Trp Gly Ala Gln Ile65 70 75 80Tyr Ala His Gln Gln Arg Ile Trp Leu Gly Thr Phe Lys Ser Glu Arg 85 90 95Glu Ala Ala Met Ala Tyr Asp Ser Ala Ser Ile Lys Leu Arg Ser Gly 100 105 110Glu Cys His Arg Asn Phe Pro Trp Asn Asp Gln Thr Val Gln Glu Pro 115 120 125Gln Phe Gln Ser His Tyr Ser Ala Glu Thr Val Leu Asn Met Ile Arg 130 135 140Asp Gly Thr Tyr Pro Ser Lys Phe Ala Thr Phe Leu Lys Thr Arg Gln145 150 155 160Thr Gln Lys Gly Val Ala Lys His Ile Gly Leu Lys Gly Asp Asp Glu 165 170 175Glu Gln Phe Cys Cys Thr Gln Leu Phe Gln Lys Glu Leu Thr Pro Ser 180 185 190Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Lys His Ala Val 195 200 205Ser Tyr Phe Pro Tyr Val Gly Gly Ser Ala Asp Glu Ser Gly Ser Val 210 215 220Asp Val Glu Ala Val Phe Tyr Asp Lys Leu Met Arg Leu Trp Lys Phe225 230 235 240Arg Tyr Cys Tyr Trp Lys Ser Ser Gln Ser Tyr Val Phe Thr Arg Gly 245 250 255Trp Asn Arg Phe Val Lys Asp Lys Lys Leu Lys Ala Lys Asp Val Ile 260 265 270Ala Phe Phe Thr Trp Gly Lys Ser Gly Gly Glu Gly Glu Ala Phe Ala 275 280 285Leu Ile Asp Val Ile Tyr Asn Asn Asn Ala Glu Glu Asp Ser Lys Gly 290 295 300Asp Thr Lys Gln Val Leu Gly Asn Gln Leu Gln Leu Ala Gly Ser Glu305 310 315 320Glu Gly Glu Asp Glu Asp Ala Asn Ile Gly Lys Asp Phe Asn Ala Gln 325 330 335Lys Gly Leu Arg Leu Phe Gly Val Cys Ile Thr 340 345243267PRTArtificial Sequencealignment 243Met Leu Arg Lys His Ile Tyr Pro Asp Glu Leu Ala Gln His Lys Arg1 5 10 15Ala Phe Phe Phe Ala Ala Ala Ser Ser Pro Thr Ser Ser Ser Ser Pro 20 25 30Leu Ala Ser Pro Ala Pro Ser Ala Ala Ala Ala Arg Arg Glu His Leu 35 40 45Phe Asp Lys Thr Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu 50 55 60Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Gln Leu Pro65 70 75 80Ser Ala Ser Ala Ala Val Pro Gly Glu Cys Lys Gly Val Leu Leu Asn 85 90 95Phe Asp Asp Ala Thr Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp 100 105 110Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val 115 120 125Lys Glu Lys Gly Leu His Ala Gly Asp Ala Val Glu Phe Tyr Arg Ala 130 135 140Ala Ser Gly Asn Asn Gln Leu Phe Ile Asp Cys Lys Leu Arg Ser Lys145 150 155 160Ser Thr Thr Thr Thr Thr Ser Val Asn Ser Glu Ala Ala Pro Ser Pro 165 170 175Ala Pro Val Thr Arg Thr Val Arg Leu Phe Gly Val Asp Leu Leu Ile 180 185 190Ala Pro Ala Ala Arg His Ala His Glu His Glu Asp Tyr Gly Met Ala 195 200 205Lys Thr Asn Lys Arg Thr Met Glu Ala Ser Val Ala Ala Pro Thr Pro 210 215 220Ala His Ala Val Trp Lys Lys Arg Cys Val Asp Phe Ala Leu Thr Tyr225 230 235 240Arg Leu Ala Thr Thr Pro Gln Cys Pro Arg Ser Arg Asp Gln Leu Glu 245 250 255Gly Val Gln Ala Ala Gly Ser Thr Phe Ala Leu 260 265244393PRTArtificial Sequencealignment 244Met Asp Ser Ser Ser Cys Leu Val Asp Asp Thr Asn Ser Gly Gly Ser1 5 10 15Ser Thr Asp Lys Leu Arg Ala Leu Ala Ala Ala Ala Ala Glu Thr Ala 20 25 30Pro Leu Glu Arg Met Gly Ser Gly Ala Ser Ala Val Val Asp Ala Ala 35 40 45Glu Pro Gly Ala Glu Ala Asp Ser Gly Ser Gly Gly Arg Val Cys Gly 50 55 60Gly Gly Gly Gly Gly Ala Gly Gly Ala Gly Gly Lys Leu Pro Ser Ser65 70 75 80Lys Phe Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln 85 90 95Ile Tyr Glu Arg His Gln Arg Val Trp Leu Gly Thr Phe Ala Gly Glu 100 105 110Asp Asp Ala Ala Arg Ala Tyr Asp Val Ala Ala Gln Arg Phe Arg Gly 115 120 125Arg Asp Ala Val Thr Asn Phe Arg Pro Leu Ala Glu Ala Asp Pro Asp 130 135 140Ala Ala Ala Glu Leu Arg Phe Leu Ala Thr Arg Ser Lys Ala Glu Val145 150 155 160Val Asp Met Leu Arg Lys His Thr Tyr Phe Asp Glu Leu Ala Gln Ser 165 170 175Lys Arg Thr Phe Ala Ala Ser Thr Pro Ser Ala Ala Thr Thr Thr Ala 180 185 190Ser Leu Ser Asn Gly His Leu Ser Ser Pro Arg Ser Pro Phe Ala Pro 195 200 205Ala Ala Ala Arg Asp His Leu Phe Asp Lys Thr Val Thr Pro Ser Asp 210 215 220Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln His Ala Glu Lys225 230 235 240His Phe Pro Leu Gln Leu Pro Ser Ala Gly Gly Glu Ser Lys Gly Val 245 250 255Leu Leu Asn Phe Glu Asp Ala Ala Gly Lys Val Trp Arg Phe Arg Tyr 260 265 270Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser 275 280 285Arg Phe Val Lys Glu Lys Gly Leu His Ala Gly Asp Val Val Gly Phe 290 295 300Tyr Arg Ser Ala Ala Ser Ala Gly Asp Asp Gly Lys Leu Phe Ile Asp305 310 315 320Cys Lys Leu Val Arg Ser Thr Gly Ala Ala Leu Ala Ser Pro Ala Asp 325 330 335Gln Pro Ala Pro Ser Pro Val Lys Ala Val Arg Leu Phe Gly Val Asp 340 345 350Leu Leu Thr Ala Pro Ala Pro Val Glu Gln Met Ala Gly Cys Lys Arg 355 360 365Ala Arg Asp Leu Ala Ala Thr Thr Pro Pro Gln Ala Ala Ala Phe Lys 370 375 380Lys Gln Cys Ile Glu Leu Ala Leu Val385 390245254PRTArtificial Sequencealignment 245Met Leu Arg Lys His Thr Tyr Phe Asp Glu Leu Ala Gln Ser Lys Arg1 5 10 15Ala Phe Ala Ala Ser Ala Ala Leu Ser Ala Pro Thr Thr Ser Gly Asp 20 25 30Ala Gly Gly Ser Ala Ser Pro Pro Ser Pro Ala Ala Val Arg Glu His 35 40 45Leu Phe Asp Lys Thr Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg 50 55 60Leu Val Ile Pro Lys Gln Asn Ala Glu Lys His Phe Pro Leu Gln Leu65 70 75 80Pro Ala Gly Gly Gly Glu Ser Lys Gly Leu Leu Leu Asn Phe Glu Asp 85 90 95Asp Ala Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 100 105 110Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 115 120 125Gly Leu Gly Ala Gly Asp Val Val Gly Phe Tyr Arg Ser Ala Ala Gly 130 135 140Arg Thr Gly Glu Asp Ser Lys Phe Phe Ile Asp Cys Arg Leu Arg Pro145 150 155 160Asn Thr Asn Thr Ala Ala Glu Ala Asp Pro Val Tyr Gly Asn Asp Thr 165 170 175Glu Asp Gln Leu Phe Ile Asp Tyr Lys Lys Met Asn Lys Asn Asp Asp 180 185 190Ala Ala Asp Ala Ala Ile Asp Gln Ser Ser Ala Pro Val Gln Lys Ala 195 200 205Val Arg Leu Phe Gly Val Asp Leu Leu Ala Ala Pro Glu Gln Gly Met 210 215 220Pro Gly Gly Cys Lys Arg Ala Arg Asp Leu Val Lys Pro Pro Pro Pro225 230 235 240Lys Val Ala Phe Lys Lys Gln Cys Ile Glu Leu Ala Leu Ala 245 250246357PRTArtificial Sequencealignment 246Met Gly Val Glu Ile Leu Ser Ser Thr Gly Glu His Ser Ser Gln Tyr1 5 10 15Ser Ser Gly Ala Ala Ser Thr Ala Thr Thr Glu Ser Gly Val Gly Gly 20 25 30Arg Pro Pro Thr Ala Pro Ser Leu Pro Val Ser Ile Ala Asp Glu Ser 35 40 45Ala Thr Ser Arg Ser Ala Ser Ala Gln Ser Thr Ser Ser Arg Phe Lys 50 55 60Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu65 70 75 80Arg His Ala Arg Val Trp Leu Gly Thr Phe Pro Asp Glu Asp Ser Ala 85 90 95Ala Arg Ala Tyr Asp Val Ala Ala Leu Arg Tyr Arg Gly Arg Glu Ala 100 105 110Ala Thr Asn Phe Pro Cys Ala Ala Ala Glu Ala Glu Leu Ala Phe Leu 115 120 125Ala Ala His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr 130 135 140Tyr Thr Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Met Gly145 150 155 160Ala Arg Ala Gln Pro Thr Pro Ser Trp Ala Arg Glu Pro Leu Phe Glu 165 170 175Lys Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val 180 185 190Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Lys Arg Thr Pro Glu 195 200 205Thr Thr Thr Thr Thr Gly Lys Gly Val Leu Leu Asn Phe Glu Asp Gly 210 215 220Glu Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln225 230 235 240Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Arg Glu Lys Gly

245 250 255Leu Gly Ala Gly Asp Ser Ile Val Phe Ser Cys Ser Ala Tyr Gly Gln 260 265 270Glu Lys Gln Phe Phe Ile Asp Cys Lys Lys Asn Lys Thr Met Thr Ser 275 280 285Cys Pro Ala Asp Asp Arg Gly Ala Ala Thr Ala Ser Pro Pro Val Ser 290 295 300Glu Pro Thr Lys Gly Glu Gln Val Arg Val Val Arg Leu Phe Gly Val305 310 315 320Asp Ile Ala Gly Glu Lys Arg Gly Arg Ala Ala Pro Val Glu Gln Glu 325 330 335Leu Phe Lys Arg Gln Cys Val Ala His Ser Gln His Ser Pro Ala Leu 340 345 350Gly Ala Phe Val Leu 355247362PRTArtificial Sequencealignment 247Met Gly Met Glu Ile Leu Ser Ser Thr Val Glu His Cys Ser Gln Tyr1 5 10 15Ser Ser Ser Ala Ser Thr Ala Thr Thr Glu Ser Gly Ala Ala Gly Arg 20 25 30Ser Thr Thr Ala Leu Ser Leu Pro Val Ala Ile Thr Asp Glu Ser Val 35 40 45Thr Ser Arg Ser Ala Ser Ala Gln Pro Ala Ser Ser Arg Phe Lys Gly 50 55 60Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ser Gln Ile Tyr Glu Arg65 70 75 80His Ala Arg Val Trp Leu Gly Thr Phe Pro Asp Gln Asp Ser Ala Ala 85 90 95Arg Ala Tyr Asp Val Ala Ser Leu Arg Tyr Arg Gly Arg Asp Ala Ala 100 105 110Thr Asn Phe Pro Cys Ala Ala Ala Glu Ala Glu Leu Ala Phe Leu Thr 115 120 125Ala His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Ala Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Ala145 150 155 160Arg Ala Gln Pro Thr Pro Ser Trp Ala Arg Val Pro Leu Phe Glu Lys 165 170 175Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro 180 185 190Lys Gln His Ala Glu Lys His Phe Pro Leu Lys Cys Thr Ala Glu Thr 195 200 205Thr Thr Thr Thr Gly Asn Gly Val Leu Leu Asn Phe Glu Asp Gly Glu 210 215 220Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser225 230 235 240Tyr Val Leu Thr Lys Gly Trp Ser Ser Phe Val Arg Glu Lys Gly Leu 245 250 255Gly Ala Gly Asp Ser Ile Val Phe Ser Ser Ser Ala Tyr Gly Gln Glu 260 265 270Lys Gln Leu Phe Ile Asn Cys Lys Lys Asn Thr Thr Met Asn Gly Gly 275 280 285Lys Thr Ala Leu Pro Leu Pro Val Val Glu Thr Ala Lys Gly Glu Gln 290 295 300Asp His Val Val Lys Leu Phe Gly Val Asp Ile Ala Gly Val Lys Arg305 310 315 320Val Arg Ala Ala Thr Gly Glu Leu Gly Pro Pro Glu Leu Phe Lys Arg 325 330 335Gln Ser Val Ala His Gly Cys Gly Arg Met Asn Tyr Ile Cys Tyr Ser 340 345 350Ile Gly Thr Ile Gly Pro Leu Met Leu Asn 355 360248351PRTArtificial Sequencealignment 248Met Gly Val Glu Ile Leu Ser Ser Met Val Glu Asp Ser Ser Gln Tyr1 5 10 15Ser Ser Gly Ala Ser Thr Ala Thr Thr Glu Ser Gly Thr Thr Gly Arg 20 25 30Ala Leu Thr Ala Leu Ser Leu Pro Val Ala Ile Ala Asp Glu Ser Val 35 40 45Thr Ser Ala Gln Ser Ala Pro Ser Arg Phe Lys Gly Val Val Pro Gln 50 55 60Pro Asn Gly Arg Trp Gly Ser Gln Ile Tyr Glu Arg His Ala Arg Val65 70 75 80Trp Leu Gly Thr Phe Pro Asp Gln Asp Leu Ala Ala Arg Ala Tyr Asp 85 90 95Val Ala Ala Leu Arg Tyr Arg Gly Arg Asp Ala Ala Thr Asn Phe Pro 100 105 110Cys Ala Ala Ala Glu Ala Glu Leu Ala Phe Leu Gly Ala His Ser Lys 115 120 125Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr Ala Asp Glu Leu 130 135 140Arg Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Ala Arg Ala Gln Pro145 150 155 160Thr Pro Ser Trp Ala Arg Glu Pro Leu Phe Glu Lys Ala Val Thr Pro 165 170 175Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln His Ala 180 185 190Glu Lys His Phe Pro Leu Lys Arg Thr Pro Glu Arg Thr Thr Thr Thr 195 200 205Gly Asn Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp 210 215 220Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr225 230 235 240Lys Gly Trp Ser Arg Phe Val Arg Glu Lys Gly Leu Ala Ala Gly Asp 245 250 255Ser Ile Ile Phe Ser Cys Ser Ala Tyr Gly Gln Glu Lys Gln Leu Phe 260 265 270Ile Asp Cys Lys Lys Asn Thr Thr Val Asn Ser Gly Lys Ser Ala Ser 275 280 285Pro Leu Pro Val Val Glu Thr Ala Lys Gly Glu Gln Val Arg Val Val 290 295 300Arg Leu Phe Gly Val Asp Ile Ala Gly Val Lys Arg Gly Arg Ala Ala305 310 315 320Thr Ala Glu Gln Gly Pro Pro Glu Leu Leu Lys Arg Gln Cys Val Pro 325 330 335Leu Pro His Gly Gln Arg Ser Pro Ala Leu Gly Ala Phe Val Leu 340 345 350249348PRTArtificial Sequencealignment 249Met Gly Val Glu Ile Leu Ser Ser Met Val Glu His Ser Phe Gln Tyr1 5 10 15Ser Ser Gly Ala Ser Ser Ala Thr Ala Glu Ser Gly Ala Val Gly Thr 20 25 30Pro Pro Arg His Leu Ser Leu Pro Val Ala Ile Ala Asp Glu Ser Leu 35 40 45Thr Ser Arg Ser Ala Ser Ser Arg Phe Lys Gly Val Val Pro Gln Pro 50 55 60Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Arg His Ala Arg Val Trp65 70 75 80Leu Gly Thr Phe Pro Asp Gln Asp Ser Ala Ala Arg Ala Tyr Asp Val 85 90 95Ala Ser Leu Arg Tyr Arg Gly Gly Asp Ala Ala Phe Asn Phe Pro Cys 100 105 110Val Val Val Glu Ala Glu Leu Ala Phe Leu Ala Ala His Ser Lys Ala 115 120 125Glu Ile Val Asp Met Leu Arg Lys Gln Thr Tyr Ala Asp Glu Leu Arg 130 135 140Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Val Arg Ala Gln Pro Met145 150 155 160Pro Ser Trp Ala Arg Val Pro Leu Phe Glu Lys Ala Val Thr Pro Ser 165 170 175Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln His Ala Glu 180 185 190Lys His Phe Pro Leu Lys Arg Ser Pro Glu Thr Thr Thr Thr Thr Gly 195 200 205Asn Gly Val Leu Leu Asn Phe Glu Asp Gly Gln Gly Lys Val Trp Arg 210 215 220Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys225 230 235 240Gly Trp Ser Arg Phe Val Arg Glu Lys Gly Leu Gly Ala Gly Asp Ser 245 250 255Ile Met Phe Ser Cys Ser Ala Tyr Gly Gln Glu Lys Gln Phe Phe Ile 260 265 270Asp Cys Lys Lys Asn Thr Thr Val Asn Gly Gly Lys Ser Ala Ser Pro 275 280 285Leu Gln Val Met Glu Ile Ala Lys Ala Glu Gln Val Arg Val Val Arg 290 295 300Leu Phe Gly Val Asp Ile Ala Gly Val Lys Arg Glu Arg Ala Ala Thr305 310 315 320Ala Glu Gln Gly Pro Gln Gly Trp Phe Lys Arg Gln Cys Met Ala His 325 330 335Gly Gln His Ser Pro Ala Leu Gly Asp Phe Ala Leu 340 345250350PRTArtificial Sequencealignment 250Met Gly Val Glu Ile Leu Ser Ser Met Val Glu His Ser Phe Gln Tyr1 5 10 15Ser Ser Gly Val Ser Thr Ala Thr Thr Glu Ser Gly Thr Ala Gly Thr 20 25 30Pro Pro Arg Pro Leu Ser Leu Pro Val Ala Ile Ala Asp Glu Ser Val 35 40 45Thr Ser Arg Ser Ala Ser Ser Arg Phe Lys Gly Val Val Pro Gln Pro 50 55 60Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Arg His Ala Arg Val Trp65 70 75 80Leu Gly Thr Phe Pro Asp Gln Asp Ser Ala Ala Arg Ala Tyr Asp Val 85 90 95Ala Ser Leu Arg Tyr Arg Gly Arg Asp Val Ala Phe Asn Phe Pro Cys 100 105 110Ala Ala Val Glu Gly Glu Leu Ala Phe Leu Ala Ala His Ser Lys Ala 115 120 125Glu Ile Val Asp Met Leu Arg Lys Gln Thr Tyr Ala Asp Glu Leu Arg 130 135 140Gln Gly Leu Arg Arg Gly Arg Gly Met Gly Ala Arg Ala Gln Pro Thr145 150 155 160Pro Ser Trp Ala Arg Glu Pro Leu Phe Glu Lys Ala Val Thr Pro Ser 165 170 175Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln His Ala Glu 180 185 190Lys His Phe Pro Leu Lys Arg Thr Pro Glu Thr Pro Thr Thr Thr Gly 195 200 205Lys Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp Arg 210 215 220Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys225 230 235 240Gly Trp Ser Arg Phe Val Arg Glu Lys Gly Leu Gly Ala Gly Asp Ser 245 250 255Ile Leu Phe Ser Cys Ser Leu Tyr Glu Gln Glu Lys Gln Phe Phe Ile 260 265 270Asp Cys Lys Lys Asn Thr Ser Met Asn Gly Gly Lys Ser Ala Ser Pro 275 280 285Leu Pro Val Gly Val Thr Thr Lys Gly Glu Gln Val Arg Val Val Arg 290 295 300Leu Phe Gly Val Asp Ile Ser Gly Val Lys Arg Gly Arg Ala Ala Thr305 310 315 320Ala Thr Ala Glu Gln Gly Leu Gln Glu Leu Phe Lys Arg Gln Cys Val 325 330 335Ala Pro Gly Gln His Ser Pro Ala Leu Gly Ala Phe Ala Leu 340 345 350251308PRTArtificial Sequencealignment 251Met Ala Ser Ser Lys Pro Thr Asn Pro Glu Val Asp Asn Asp Met Glu1 5 10 15Cys Ser Ser Pro Glu Ser Gly Ala Glu Asp Ala Val Glu Ser Ser Ser 20 25 30Pro Val Ala Ala Pro Ser Ser Arg Phe Lys Gly Val Val Pro Gln Pro 35 40 45Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Lys His Ser Arg Val Trp 50 55 60Leu Gly Thr Phe Gly Asp Glu Glu Ala Ala Ala Cys Ala Tyr Asp Val65 70 75 80Ala Ala Leu Arg Phe Arg Gly Arg Asp Ala Val Thr Asn His Gln Arg 85 90 95Leu Pro Ala Ala Glu Gly Ala Gly Trp Ser Ser Thr Ser Glu Leu Ala 100 105 110Phe Leu Ala Asp His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys 115 120 125His Thr Tyr Asp Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly His Gly 130 135 140Arg Ala Gln Pro Thr Pro Ala Trp Ala Arg Glu Phe Leu Phe Glu Lys145 150 155 160Ala Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro 165 170 175Lys Gln His Ala Glu Lys His Phe Pro Pro Thr Thr Ala Ala Ala Ala 180 185 190Gly Ser Asp Gly Lys Gly Leu Leu Leu Asn Phe Glu Asp Gly Gln Gly 195 200 205Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr 210 215 220Val Leu Thr Lys Gly Trp Ser Arg Phe Val Gln Glu Lys Gly Leu Cys225 230 235 240Ala Gly Asp Thr Val Thr Phe Ser Arg Ser Ala Tyr Val Met Asn Asp 245 250 255Thr Asp Glu Gln Leu Phe Ile Asp Tyr Lys Gln Ser Ser Lys Asn Asp 260 265 270Glu Ala Ala Asp Val Ala Thr Ala Asp Glu Asn Glu Ala Gly His Val 275 280 285Ala Val Lys Leu Phe Gly Val Asp Ile Gly Trp Ala Gly Met Ala Gly 290 295 300Ser Ser Gly Gly305252293PRTArtificial Sequencealignment 252Met Ala Ser Gly Lys Pro Thr Asn His Gly Met Glu Asp Asp Asn Asp1 5 10 15Met Glu Tyr Ser Ser Ala Glu Ser Gly Ala Glu Asp Ala Ala Glu Pro 20 25 30Ser Ser Ser Pro Val Leu Ala Pro Pro Arg Ala Ala Pro Ser Ser Arg 35 40 45Phe Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile 50 55 60Tyr Glu Lys His Ser Arg Val Trp Leu Gly Thr Phe Pro Asp Glu Asp65 70 75 80Ala Ala Val Arg Ala Tyr Asp Val Ala Ala Leu Arg Phe Arg Gly Pro 85 90 95Asp Ala Val Ile Asn His Gln Arg Pro Thr Ala Ala Glu Glu Ala Gly 100 105 110Ser Ser Ser Ser Arg Ser Glu Leu Asp Pro Glu Leu Gly Phe Leu Ala 115 120 125Asp His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Asp Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Arg Ala Gln145 150 155 160Pro Thr Pro Ala Trp Ala Arg Glu Leu Leu Phe Glu Lys Ala Val Thr 165 170 175Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln Gln 180 185 190Ala Glu Lys His Phe Pro Pro Thr Thr Ala Ala Ala Thr Gly Ser Asn 195 200 205Gly Lys Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp 210 215 220Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr225 230 235 240Lys Gly Trp Ser Arg Phe Val Lys Glu Thr Gly Leu Arg Ala Gly Asp 245 250 255Thr Val Ala Phe Tyr Arg Ser Ala Ser Asp Glu Asn Glu Thr Gly His 260 265 270Val Ala Val Lys Leu Phe Gly Val Asp Ile Ala Gly Gly Gly Met Ala 275 280 285Gly Ser Ser Gly Gly 290253320PRTArtificial Sequencealignment 253Met Ala Ser Gly Lys Pro Thr Asn His Gly Met Glu Asp Asp Asn Asp1 5 10 15Met Glu Tyr Ser Ser Ala Glu Ser Gly Ala Glu Asp Ala Ala Glu Pro 20 25 30Ser Ser Ser Pro Val Leu Ala Pro Pro Arg Ala Ala Pro Ser Ser Arg 35 40 45Phe Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile 50 55 60Tyr Glu Lys His Ser Arg Val Trp Leu Gly Thr Phe Pro Asp Glu Asp65 70 75 80Ala Ala Ala Arg Ala Tyr Asp Val Ala Ala Leu Arg Phe Arg Gly Pro 85 90 95Asp Ala Val Ile Asn His Gln Arg Pro Thr Ala Ala Glu Glu Ala Gly 100 105 110Ser Ser Ser Ser Arg Ser Glu Leu Asp Pro Glu Leu Gly Phe Leu Ala 115 120 125Asp His Ser Lys Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Asp Asp Glu Leu Arg Gln Gly Leu Arg Arg Gly Arg Gly Arg Ala Gln145 150 155 160Pro Thr Pro Ala Trp Ala Arg Glu Leu Leu Phe Glu Lys Ala Val Thr 165 170 175Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Val Pro Lys Gln Gln 180 185 190Ala Glu Lys His Phe Pro Pro Thr Thr Ala Ala Ala Thr Gly Ser Asn 195 200 205Gly Lys Gly Val Leu Leu Asn Phe Glu Asp Gly Glu Gly Lys Val Trp 210 215 220Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr225 230 235 240Lys Gly Trp Ser Arg Phe Val Lys Glu Thr Gly Leu Arg Ala Gly Asp 245 250 255Thr Val Ala Phe Tyr Arg Ser Ala Tyr Gly Asn Asp Thr Glu Asp Gln 260 265 270Leu Phe Ile Asp Tyr Lys Lys Met Asn Lys Asn Asp Asp Ala Ala Asp 275 280 285Ala Ala Ile Ser Asp Glu Asn Glu Thr Gly His Val Ala Val Lys Leu 290 295 300Phe Gly Val Asp Ile Ala Gly Gly Gly Met Ala Gly Ser Ser Gly Gly305 310 315 320254350PRTArtificial

Sequencealignment 254Met Val Phe Ser Cys Ile Asp Glu Ser Ser Ser Thr Ser Glu Ser Phe1 5 10 15Ser Pro Ala Thr Ala Thr Ala Thr Ala Thr Ala Thr Lys Phe Ser Ala 20 25 30Pro Pro Leu Pro Pro Leu Arg Leu Asn Arg Met Arg Ser Gly Gly Ser 35 40 45Asn Val Val Leu Asp Ser Lys Asn Gly Val Asp Ile Asp Ser Arg Lys 50 55 60Leu Ser Ser Ser Lys Tyr Lys Gly Val Val Pro Gln Pro Asn Gly Arg65 70 75 80Trp Gly Ala Gln Ile Tyr Val Lys His Gln Arg Val Trp Leu Gly Thr 85 90 95Phe Cys Asp Glu Glu Glu Ala Ala His Ser Tyr Asp Ile Ala Ala Arg 100 105 110Lys Phe Arg Gly Arg Asp Ala Val Val Asn Phe Lys Thr Phe Leu Ala 115 120 125Ser Glu Asp Asp Asn Gly Glu Leu Cys Phe Leu Glu Ala His Ser Lys 130 135 140Ala Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr Ala Asp Glu Leu145 150 155 160Ala Gln Ser Asn Lys Arg Ser Gly Ala Asn Thr Asn Thr Asn Thr Thr 165 170 175Gln Ser His Thr Val Ser Arg Thr Arg Glu Val Leu Phe Glu Lys Val 180 185 190Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys 195 200 205Gln His Ala Glu Lys Tyr Phe Pro Leu Pro Ser Leu Ser Val Thr Lys 210 215 220Gly Val Leu Ile Asn Phe Glu Asp Val Thr Gly Lys Val Trp Arg Phe225 230 235 240Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly 245 250 255Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Arg Ala Gly Asp Val Val 260 265 270Thr Phe Glu Arg Ser Thr Gly Ser Asp Arg Gln Leu Tyr Ile Asp Trp 275 280 285Lys Ile Arg Ser Gly Pro Ser Lys Asn Pro Val Gln Val Val Val Arg 290 295 300Leu Phe Gly Val Asp Ile Phe Asn Val Thr Ser Ala Lys Pro Ser Asn305 310 315 320Val Val Asp Ala Cys Gly Gly Lys Arg Ser Arg Asp Val Asp Met Phe 325 330 335Ala Leu Arg Cys Ser Lys Lys His Ala Ile Ile Asn Ala Leu 340 345 350255351PRTArtificial Sequencealignment 255Met Asp Gly Gly Cys Val Thr Asp Glu Thr Thr Thr Ser Ser Asp Ser1 5 10 15Leu Ser Val Pro Pro Pro Ser Arg Val Gly Ser Val Ala Ser Ala Val 20 25 30Val Asp Pro Asp Gly Cys Cys Val Ser Gly Glu Ala Glu Ser Arg Lys 35 40 45Leu Pro Ser Ser Lys Tyr Lys Gly Val Val Pro Gln Pro Asn Gly Arg 50 55 60Trp Gly Ala Gln Ile Tyr Glu Lys His Gln Arg Val Trp Leu Gly Thr65 70 75 80Phe Asn Glu Glu Asp Glu Ala Ala Arg Ala Tyr Asp Ile Ala Ala Leu 85 90 95Arg Phe Arg Gly Pro Asp Ala Val Thr Asn Phe Lys Pro Pro Ala Ala 100 105 110Ser Asp Asp Ala Glu Ser Glu Phe Leu Asn Ser His Ser Lys Phe Glu 115 120 125Ile Val Asp Met Leu Arg Lys His Thr Tyr Asp Asp Glu Leu Gln Gln 130 135 140Ser Thr Arg Gly Gly Arg Arg Arg Leu Asp Ala Asp Thr Ala Ser Ser145 150 155 160Gly Val Phe Asp Ala Lys Ala Arg Glu Gln Leu Phe Glu Lys Thr Val 165 170 175Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys Gln 180 185 190His Ala Glu Lys His Phe Pro Leu Ser Gly Ser Gly Asp Glu Ser Ser 195 200 205Pro Cys Val Ala Gly Ala Ser Ala Ala Lys Gly Met Leu Leu Asn Phe 210 215 220Glu Asp Val Gly Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn225 230 235 240Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys 245 250 255Glu Lys Asn Leu Arg Ala Gly Asp Ala Val Gln Phe Phe Lys Ser Thr 260 265 270Gly Pro Asp Arg Gln Leu Tyr Ile Asp Cys Lys Ala Arg Ser Gly Glu 275 280 285Val Asn Asn Asn Ala Gly Gly Leu Phe Val Pro Ile Gly Pro Val Val 290 295 300Glu Pro Val Gln Met Val Arg Leu Phe Gly Val Asn Leu Leu Lys Leu305 310 315 320Pro Val Pro Gly Ser Asp Gly Val Gly Lys Arg Lys Glu Met Glu Leu 325 330 335Phe Ala Phe Glu Cys Cys Lys Lys Leu Lys Val Ile Gly Ala Leu 340 345 350256362PRTArtificial Sequencealignment 256Met Asp Gly Gly Ser Val Thr Asp Glu Thr Thr Thr Thr Ser Asn Ser1 5 10 15Leu Ser Val Pro Ala Asn Leu Ser Pro Pro Pro Leu Ser Leu Val Gly 20 25 30Ser Gly Ala Thr Ala Val Val Tyr Pro Asp Gly Cys Cys Val Ser Gly 35 40 45Glu Ala Glu Ser Arg Lys Leu Pro Ser Ser Lys Tyr Lys Gly Val Val 50 55 60Pro Gln Pro Asn Gly Arg Trp Gly Ala Gln Ile Tyr Glu Lys His Gln65 70 75 80Arg Val Trp Leu Gly Thr Phe Asn Glu Glu Asp Glu Ala Ala Arg Ala 85 90 95Tyr Asp Ile Ala Ala His Arg Phe Arg Gly Arg Asp Ala Val Thr Asn 100 105 110Phe Lys Pro Leu Ala Gly Ala Asp Asp Ala Glu Ala Glu Phe Leu Ser 115 120 125Thr His Ser Lys Ser Glu Ile Val Asp Met Leu Arg Lys His Thr Tyr 130 135 140Asp Asn Glu Leu Gln Gln Ser Thr Arg Gly Gly Arg Arg Arg Arg Asp145 150 155 160Ala Glu Thr Ala Ser Ser Gly Ala Phe Asp Ala Lys Ala Arg Glu Gln 165 170 175Leu Phe Glu Lys Thr Val Thr Gln Ser Asp Val Gly Lys Leu Asn Arg 180 185 190Leu Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Ser Gly 195 200 205Ser Gly Gly Gly Ala Leu Pro Cys Met Ala Ala Ala Ala Gly Ala Lys 210 215 220Gly Met Leu Leu Asn Phe Glu Asp Val Gly Gly Lys Val Trp Arg Phe225 230 235 240Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly 245 250 255Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Arg Ala Gly Asp Ala Val 260 265 270Gln Phe Phe Lys Ser Thr Gly Leu Asp Arg Gln Leu Tyr Ile Asp Cys 275 280 285Lys Ala Arg Ser Gly Lys Val Asn Asn Asn Ala Ala Gly Leu Phe Ile 290 295 300Pro Val Gly Pro Val Val Glu Pro Val Gln Met Val Arg Leu Phe Gly305 310 315 320Val Asp Leu Leu Lys Leu Pro Val Pro Gly Ser Asp Gly Ile Gly Val 325 330 335Gly Cys Asp Gly Lys Arg Lys Glu Met Glu Leu Phe Ala Phe Glu Cys 340 345 350Ser Lys Lys Leu Lys Val Ile Gly Ala Leu 355 360257384PRTArtificial Sequencealignment 257Met Asp Ala Ile Ser Cys Leu Asp Glu Ser Thr Thr Thr Glu Ser Leu1 5 10 15Ser Ile Ser Gln Ala Lys Pro Ser Ser Thr Ile Met Ser Ser Glu Lys 20 25 30Ala Ser Pro Ser Pro Pro Pro Pro Asn Arg Leu Cys Arg Val Gly Ser 35 40 45Gly Ala Ser Ala Val Val Asp Ser Asp Gly Gly Gly Gly Gly Gly Ser 50 55 60Thr Glu Val Glu Ser Arg Lys Leu Pro Ser Ser Lys Tyr Lys Gly Val65 70 75 80Val Pro Gln Pro Asn Gly Arg Trp Gly Ser Gln Ile Tyr Glu Lys His 85 90 95Gln Arg Val Trp Leu Gly Thr Phe Asn Glu Glu Asp Glu Ala Ala Arg 100 105 110Ala Tyr Asp Val Ala Val Gln Arg Phe Arg Gly Lys Asp Ala Val Thr 115 120 125Asn Phe Lys Pro Leu Ser Gly Thr Asp Asp Asp Asp Gly Glu Ser Glu 130 135 140Phe Leu Asn Ser His Ser Lys Ser Glu Ile Val Asp Met Leu Arg Lys145 150 155 160His Thr Tyr Asn Asp Glu Leu Glu Gln Ser Lys Arg Ser Arg Gly Phe 165 170 175Val Arg Arg Arg Gly Ser Ala Ala Gly Ala Gly Asn Gly Asn Ser Ile 180 185 190Ser Gly Ala Cys Val Met Lys Ala Arg Glu Gln Leu Phe Gln Lys Ala 195 200 205Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu Val Ile Pro Lys 210 215 220Gln His Ala Glu Lys His Phe Pro Leu Gln Ser Ala Ala Asn Gly Val225 230 235 240Ser Ala Thr Ala Thr Ala Ala Lys Gly Val Leu Leu Asn Phe Glu Asp 245 250 255Val Gly Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 260 265 270Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys 275 280 285Asn Leu Lys Ala Gly Asp Thr Val Cys Phe Gln Arg Ser Thr Gly Pro 290 295 300Asp Arg Gln Leu Tyr Ile Asp Trp Lys Thr Arg Asn Val Val Asn Glu305 310 315 320Val Ala Leu Phe Gly Pro Val Val Glu Pro Ile Gln Met Val Arg Leu 325 330 335Phe Gly Val Asn Ile Leu Lys Leu Pro Gly Ser Asp Ser Ile Ala Asn 340 345 350Asn Asn Asn Ala Ser Gly Cys Cys Asn Gly Lys Arg Arg Glu Met Glu 355 360 365Leu Phe Ser Leu Glu Cys Ser Lys Lys Pro Lys Ile Ile Gly Ala Leu 370 375 380258401PRTArtificial Sequencealignment 258Met Asp Ala Ile Ser Cys Met Asp Glu Ser Thr Thr Thr Glu Ser Leu1 5 10 15Ser Ile Ser Leu Ser Pro Thr Ser Ser Ser Glu Lys Ala Lys Pro Ser 20 25 30Ser Met Ile Thr Ser Ser Glu Lys Val Ser Leu Ser Pro Pro Pro Ser 35 40 45Asn Arg Leu Cys Arg Val Gly Ser Gly Ala Ser Ala Val Val Asp Pro 50 55 60Asp Gly Gly Gly Ser Gly Ala Glu Val Glu Ser Arg Lys Leu Pro Ser65 70 75 80Ser Lys Tyr Lys Gly Val Val Pro Gln Pro Asn Gly Arg Trp Gly Ala 85 90 95Gln Ile Tyr Glu Lys His Gln Arg Val Trp Leu Gly Thr Phe Asn Glu 100 105 110Glu Asp Glu Ala Ala Arg Ala Tyr Asp Ile Ala Ala Gln Arg Phe Arg 115 120 125Gly Lys Asp Ala Val Thr Asn Phe Lys Pro Leu Ala Gly Ala Asp Asp 130 135 140Asp Asp Gly Glu Ser Glu Phe Leu Asn Ser His Ser Lys Pro Glu Ile145 150 155 160Val Asp Met Leu Arg Lys His Thr Tyr Asn Asp Glu Leu Glu Gln Ser 165 170 175Lys Arg Ser Arg Gly Val Val Arg Arg Arg Gly Ser Ala Ala Ala Gly 180 185 190Thr Ala Asn Ser Ile Ser Gly Ala Cys Phe Thr Lys Ala Arg Glu Gln 195 200 205Leu Phe Glu Lys Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg 210 215 220Leu Val Ile Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Gln Ser225 230 235 240Ser Asn Gly Val Ser Ala Thr Thr Ile Ala Ala Val Thr Ala Thr Pro 245 250 255Thr Ala Ala Lys Gly Val Leu Leu Asn Phe Glu Asp Val Gly Gly Lys 260 265 270Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 275 280 285Leu Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Lys Ala 290 295 300Gly Asp Thr Val Cys Phe His Arg Ser Thr Gly Pro Asp Lys Gln Leu305 310 315 320Tyr Ile Asp Trp Lys Thr Arg Asn Val Val Asn Asn Glu Val Ala Leu 325 330 335Phe Gly Pro Val Gly Pro Val Val Glu Pro Ile Gln Met Val Arg Leu 340 345 350Phe Gly Val Asn Ile Leu Lys Leu Pro Gly Ser Asp Thr Ile Val Gly 355 360 365Asn Asn Asn Asn Ala Ser Gly Cys Cys Asn Gly Lys Arg Arg Glu Met 370 375 380Glu Leu Phe Ser Leu Glu Cys Ser Lys Lys Pro Lys Ile Ile Gly Ala385 390 395 400Leu259192PRTArtificial Sequencealignment 259Met Ala Met His Ala Gly His Ala Trp Trp Gly Val Ala Met Tyr Thr1 5 10 15Asn His Tyr His His His Tyr Arg His Lys Thr Ser Asp Val Gly Lys 20 25 30Asn Arg Val Lys His Ala Arg Tyr Gly Gly Gly Asp Ser Gly Lys Gly 35 40 45Ser Asp Ser Gly Lys Trp Arg Arg Tyr Ser Tyr Trp Thr Ser Ser Ser 50 55 60Tyr Val Thr Lys Gly Trp Ser Arg Tyr Val Lys Lys Arg Asp Ala Gly65 70 75 80Asp Val Val His Arg Val Arg Gly Gly Ala Ala Asp Arg Gly Cys Arg 85 90 95Arg Arg Gly Ser Ala Ala Ala Val Arg Val Thr Ala Asn Gly Gly Trp 100 105 110Ser Met Cys Tyr Ser Thr Ser Gly Ser Ser Tyr Asp Thr Ser Ala Asn 115 120 125Ser Tyr Ala Tyr His Arg Ser Val Asp Asp His Ser Asp His Ala Gly 130 135 140Ser Arg Ala Asp Ala Lys Ser Ser Ser Ala Ala Ser Ala Ser Arg Arg145 150 155 160Arg Gly Val Asn Asp Cys Gly Ala Asp Ala Thr Ala Met Tyr Gly Tyr 165 170 175Met His His Ser Tyr Ala Ala Val Ser Thr Val Asn Tyr Trp Ser Val 180 185 190260116PRTArtificial Sequencealignment 260Phe Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Asn Asn 20 25 30Asn Asn Asn Gly Gly Ser Gly Asp Asp Val Ala Thr Thr Glu Lys Gly 35 40 45Met Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys Cys Trp Lys Phe Arg 50 55 60Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp65 70 75 80Ser Arg Tyr Val Lys Asp Lys His Leu Asp Ala Gly Asp Val Val Phe 85 90 95Phe Gln Arg His Arg Phe Asp Leu His Arg Leu Phe Ile Gly Trp Arg 100 105 110Arg Arg Gly Glu 115261114PRTArtificial Sequencealignment 261Phe Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Arg Tyr Leu Pro Leu Asn Asn Cys 20 25 30Gly Gly Gly Gly Asp Val Thr Ala Glu Ser Thr Glu Lys Gly Val Leu 35 40 45Leu Ser Phe Glu Asp Glu Ser Gly Lys Ser Trp Lys Phe Arg Tyr Ser 50 55 60Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg65 70 75 80Tyr Val Lys Asp Lys His Leu Asn Ala Gly Asp Val Val Leu Phe Gln 85 90 95Arg His Arg Phe Asp Ile His Arg Leu Phe Ile Gly Trp Arg Arg Arg 100 105 110Gly Glu262106PRTArtificial Sequencealignment 262Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Ser Gly Asp 20 25 30Ser Gly Gly Ser Glu Cys Lys Gly Leu Leu Leu Ser Phe Glu Asp Glu 35 40 45Ser Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln 50 55 60Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys Arg65 70 75 80Leu Asp Ala Gly Asp Val Val Leu Phe Glu Arg His Arg Val Asp Ala 85 90 95Gln Arg Leu Phe Ile Gly Trp Arg Arg Arg 100 105263107PRTArtificial Sequencealignment 263Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Ser Gly Gly 20 25 30Asp Ser Gly Ser Ser Glu Cys Lys Gly Leu Leu Leu Ser Phe Glu Asp 35 40

45Glu Ser Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 50 55 60Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys65 70 75 80Arg Leu Asp Ala Gly Asp Val Val Leu Phe Gln Arg His Arg Ala Asp 85 90 95Ala Gln Arg Leu Phe Ile Gly Trp Arg Arg Arg 100 105264107PRTArtificial Sequencealignment 264Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ser Ser 20 25 30Gly Gly Asp Ser Ala Ala Ala Lys Gly Leu Leu Leu Ser Phe Glu Asp 35 40 45Glu Ser Gly Lys Cys Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser 50 55 60Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Asp Lys65 70 75 80Arg Leu His Ala Gly Asp Val Val Leu Phe His Arg His Arg Ala His 85 90 95Pro Gln Arg Phe Phe Ile Ser Cys Thr Arg His 100 105265108PRTArtificial Sequencealignment 265Phe Glu Lys Pro Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Arg Tyr Phe Pro Leu Gly Gly Gly 20 25 30Asp Ser Gly Glu Lys Gly Leu Leu Leu Ser Phe Glu Asp Glu Ser Gly 35 40 45Lys Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Thr Ser Ser Gln Ser Tyr 50 55 60Val Leu Thr Lys Gly Trp Ser Arg Tyr Val Lys Glu Lys Arg Leu Asp65 70 75 80Ala Gly Asp Val Val His Phe Glu Arg Val Arg Gly Leu Gly Ala Ala 85 90 95Asp Arg Leu Phe Ile Gly Cys Arg Arg Arg Gly Glu 100 105266115PRTArtificial Sequencealignment 266Phe Glu Lys Ser Leu Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asn Ala Val 20 25 30Leu Val Ser Ser Ala Ala Ala Asp Thr Ser Ser Ser Glu Lys Gly Met 35 40 45Leu Leu Ser Phe Glu Asp Glu Ser Gly Lys Ser Trp Arg Phe Arg Tyr 50 55 60Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser65 70 75 80Arg Phe Val Lys Asp Lys Gln Leu Asp Pro Gly Asp Val Val Phe Phe 85 90 95Gln Arg His Arg Ser Asp Ser Arg Arg Leu Phe Ile Gly Trp Arg Arg 100 105 110Arg Gly Gln 115267107PRTArtificial Sequencealignment 267Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala 20 25 30Ala Asn Glu Lys Gly Leu Leu Leu Ser Phe Glu Asp Arg Gly Gly Lys 35 40 45Leu Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 50 55 60Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala65 70 75 80Gly Asp Thr Val Ser Phe Cys Arg Gly Ala Ala Asp Ala Thr Arg Asp 85 90 95Arg Leu Phe Ile Asp Trp Lys Arg Arg Val Glu 100 105268105PRTArtificial Sequencealignment 268Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala 20 25 30Ala Asn Glu Lys Gly Leu Leu Leu Ser Phe Glu Asp Arg Ala Gly Lys 35 40 45Leu Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 50 55 60Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala65 70 75 80Gly Asp Thr Val Ser Phe Cys Arg Gly Ala Ala Asp Ala Ala Arg Asp 85 90 95Arg Leu Phe Ile Asp Trp Arg Lys Arg 100 105269107PRTArtificial Sequencealignment 269Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ala 20 25 30Ala Asn Glu Lys Gly Gln Leu Leu Ser Phe Glu Asp Arg Ala Gly Lys 35 40 45Leu Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 50 55 60Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala65 70 75 80Gly Asp Thr Val Ser Phe Cys Arg Gly Ala Gly Asp Thr Ala Arg Asp 85 90 95Arg Leu Phe Ile Asp Trp Lys Arg Arg Ala Asp 100 105270107PRTArtificial Sequencealignment 270Phe Asp Lys Val Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Asp Ala Ser 20 25 30Ser Thr Asp Lys Gly Leu Leu Leu Ser Phe Glu Asp Arg Ala Gly Lys 35 40 45Pro Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr Val 50 55 60Met Thr Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Arg Leu Asp Ala65 70 75 80Gly Asp Thr Val Ser Phe Gly Arg Gly Val Gly Glu Ala Ala Arg Gly 85 90 95Arg Leu Phe Ile Asp Trp Arg Arg Arg Pro Asp 100 105271104PRTArtificial Sequencealignment 271Phe Glu Lys Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Ile Pro Lys Gln His Ala Glu Lys Tyr Phe Pro Leu Gln Ser Gly 20 25 30Ser Ala Ser Ser Lys Gly Val Leu Leu Asn Phe Glu Asp Val Thr Gly 35 40 45Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser Ser Gln Ser Tyr 50 55 60Val Leu Ile Lys Gly Trp Ser Arg Phe Val Lys Glu Lys Asn Leu Lys65 70 75 80Ala Gly Asp Ile Val Ser Phe Gln Arg Ser Thr Gly Thr Glu Lys Gln 85 90 95Leu Tyr Ile Asp Trp Lys Ala Arg 100272102PRTArtificial Sequencealignment 272Phe Glu Lys Ala Val Thr Pro Ser Asp Val Gly Lys Leu Asn Arg Leu1 5 10 15Val Val Pro Lys Gln His Ala Glu Lys His Phe Pro Leu Lys Arg Thr 20 25 30Pro Glu Thr Pro Thr Thr Thr Gly Lys Gly Val Leu Leu Asn Phe Glu 35 40 45Asp Gly Glu Gly Lys Val Trp Arg Phe Arg Tyr Ser Tyr Trp Asn Ser 50 55 60Ser Gln Ser Tyr Val Leu Thr Lys Gly Trp Ser Arg Phe Val Arg Glu65 70 75 80Lys Gly Leu Gly Ala Gly Asp Ser Ile Leu Phe Ser Cys Ser Leu Tyr 85 90 95Glu Gln Glu Lys Gln Phe 100

Claims

1.-16. (canceled)

17. A method for altering a plant phenotype comprising reducing or abolishing the expression of a nucleic acid sequence encoding a NGAL2 polypeptide or reducing or abolishing the activity of a NGAL2 polypeptide, or reducing or abolishing the expression of a nucleic acid sequences encoding a NGAL3 polypeptide, or reducing or abolishing the activity of a NGAL3 polypeptide, or reducing or abolishing the expression of nucleic acid sequences encoding NGAL2 and NGAL3 polypeptides or reducing or abolishing the activity of a NGAL2 and NGAL3 polypeptide, relative to a control plant.

18.-22. (canceled)

23. The method according to claim 17, wherein the NGAL2 polypeptide comprises SEQ D NO: 3, a functional variant or homologue thereof.

24. The method according to claim 17, wherein the nucleic acid sequence encoding a NGAL2 polypeptide comprises SEQ ID NO: 1 or 2, a functional variant or homologue thereof.

25. The method according to claim 24 wherein the functional variant or homologue comprises a nucleic acid sequence as shown in SEQ ID NO: 49-145.

26. The method according to claim 17, wherein the NGAL3 polypeptide comprises SEQ ID NO: 5, a functional variant or homologue thereof.

27. The method according to claim 17 wherein the NGAL3 nucleic acid sequence encoding a NGAL3 polypeptide comprises SEQ ID NO: 4, a functional variant or homologue thereof.

28. The method according to claim 27 wherein the functional variant or homologue comprises SEQ ID NOs:49-145.

29.-33. (canceled)

34. The method according to claim 17, wherein said phenotype is characterised by increased seed size relative to a control plant.

35.-36. (canceled)

37. A vector comprising SEQ ID NO: 1, 2 or 3 or a functional variant or homolog thereof.

38. (canceled)