SIMIAN ADENOVIRAL VECTORS WITH TWO EXPRESSION CASSETTES

Abstract

A simian adenoviral vector comprising two expression cassettes, wherein each expression cassette comprises a transgene and a promoter, and wherein the first expression cassette is inserted in the E1 region of the simian adenoviral vector, and the second expression cassette is inserted in a region of the adenoviral vector that is compatible with vector replication.

Description

SEQUENCE LISTING

[0001] The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Oct. 11, 2018, is named VU66441A_WO_SL.txt and is 178,926 bytes in size.

FIELD OF THE INVENTION

[0002] This invention is in the field of recombinant adenoviral vectors. The invention relates to an adenoviral vector comprising two expression cassettes. In particular, the invention relates to a simian adenovirus such as a chimpanzee (chimp) adenovirus comprising two expression cassettes.

BACKGROUND OF THE INVENTION

[0003] Recombinant adenoviruses are useful in gene therapy and as vaccines.

[0004] Human adenoviruses have been widely used for gene transfer applications due to their large transgene capacity and ability to achieve highly efficient gene transfer in a variety of target tissues.

[0005] However, most humans are exposed to and develop immunity to human adenoviruses. Therefore, there is a demand for vectors which effectively deliver molecules to a target and minimize the effect of pre-existing immunity to human adenovirus serotypes. Simian adenoviruses are effective in this regard; they are sufficiently closely related to human viruses to be effective in inducing immunity to delivered exogenous antigens to which humans have little or no pre-existing immunity. Therefore, viral vectors based on simian adenoviruses can provide an alternative to the use of human derived adenoviral vectors for the development of nucleic acid based vaccines.

[0006] Replication defective adenoviruses deliver their genome to the interior of a cell and, because they do not replicate, do not amplify the transgene payload. Typically, the E1 gene is replaced with a transgene cassette comprising a promoter of choice and a nucleic acid sequence corresponding to a gene or genes of interest, resulting in a replication defective recombinant virus.

[0007] There is a need in the art for improved recombinant adenoviruses.

SUMMARY OF THE INVENTION

[0008] The invention relates to a simian adenoviral vector comprising two expression cassettes. In particular, the invention relates to a simian adenovirus such as a chimpanzee (chimp) adenovirus comprising two expression cassettes. Examples of suitable chimp adenoviruses include ChAd155 and ChAd83.

[0009] The adenoviral vectors of the invention are useful as components of immunogenic compostions for the induction of an immune response in a subject, methods for their use in treatment and processes for manufacture.

[0010] The term "vector" refers to an agent (such as a plasmid or virus) that contains or carries genetic material and can be used to introduce exogenous genes into an organism. The adenoviral vector of the present invention is derived from a non-human simian adenovirus, also referred to as a "simian adenovirus". Preferably, the simian adenoviral vector of the present invention is a simian adenovirus.

[0011] Each expression cassette in the adenoviral vector of the invention comprises a transgene and a promoter. A "transgene" is a nucleic acid sequence, heterologous to the vector sequences flanking the transgene, which encodes a polypeptide of interest. The nucleic acid coding sequence is operatively linked to regulatory components in a manner which permits transgene transcription, translation, and/or expression in a host cell. A "promoter" is a nucleotide sequence that permits the binding of RNA polymerase and directs the transcription of a gene. Typically, a promoter is located in a non-coding region of a gene, proximal to the transcriptional start site.

[0012] In adenoviral vectors of the invention, the first expression cassette is inserted in the E1 region of the virus, and the second expression cassette is inserted into a second region of the adenoviral vector.

[0013] In a simian adenoviral vector comprising two expression cassettes of the invention, the first expression cassette is inserted in the E1 region of the simian adenoviral vector, and the second expression cassette is inserted in a region of the adenoviral vector that is compatible with vector replication. A region of the adenoviral vector genome is considered "compatible with vector replication" if disruption of this region would not affect the ability of the adenoviral vector to replicate.

[0014] Preferably, in adenoviral vectors of the invention, the first expression cassette is inserted in the E1 region of the virus, and the second expression cassette is inserted into the E3, HE1 or HE2 region of the adenoviral vector. As is well known in the art, the E3 genes are expressed in the early phase of transduction to prepare the host cell for viral replication. E3 is involved in immune modulation. The term "HE1" is used to describe a site located between the stop codons of L5 and E4. The term "HE2" has been used to define a site located between the end of the ITR and the cap site of E4 mRNA.

[0015] For example, in a ChAd155 adenovirus vector:

[0016] HE1 ChAd155: insertion site between bp 34611 and 34612 of SEQ ID NO: 1.

[0017] HE2 ChAd155: insertion site between bp 37662 and 37663 of SEQ ID NO: 1.

[0018] In another example, in a ChAd83 adenovirus vector:

[0019] HE1 ChAd83: insertion site between bp 33535 and 33536 of SEQ ID NO: 2.

[0020] HE2 ChAd83: insertion site between bp 36387 and 36388 of SEQ ID NO: 2.

[0021] As the first expression cassette is inserted in the E1 region of the adenoviral vector, the native E1 region is deleted. In order to increase the cloning capacity of the vector, the native E3 region can be removed from the adenoviral vector. The native E3 region can be deleted from the adenoviral vector in embodiments of the invention where the second expression cassette is inserted in the E3 region, or in embodiments where the second expression cassette is not inserted into the E3 region. The insertion in HE1 or HE2 site doesn't require deletion of any specific sequence of the vector backbone.

[0022] Preferably, the second expression cassette is inserted into the HE1 or HE2 region of the adenoviral vector. Most preferably, the second expression cassette is inserted in the HE2 region of the adenoviral vector. In one embodiment, the native E3 region is deleted from the adenoviral vector to increase the cloning capacity of the vector, and the second expression cassette is inserted in the HE1 or HE2 region of the adenoviral vector.

[0023] In embodiments of the invention, the first expression cassette of the adenoviral vector may comprise a human CMV or an enhanced human CMV promoter, and/or the second expression cassette may comprise a human CMV or an enhanced human CMV promoter.

[0024] In a preferred embodiment, the first and second expression cassettes comprise different promoters. For example, in one embodiment, the first expression cassette may comprise a human CMV promoter and the second expression cassette an enhanced human CMV promoter (or vice versa).

[0025] In one aspect of the invention, there is provided an adenoviral vector of the invention, wherein the first expression cassette is inserted in the E1 region of the virus, and the second expression cassette is inserted in a region of the adenoviral vector that is compatible with vector replication, wherein at least one of the first and second expression cassette comprises an enhanced CMV promoter. In some embodiments, the enhanced hCMV promoter can include a nucleic acid sequence having at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more, sequence identity to SEQ ID NO: 6. In some embodiments, the promoter comprises or consists of a nucleic acid sequence of SEQ ID NO: 6.

[0026] Adenoviral vectors of the invention are derived from a simian adenoviral vector, for example, from chimpanzees (Pan troglodytes), bonobos (Pan paniscus), gorillas (Gorilla gorilla) and orangutans (Pongo abelii and Pongo pygnaeus). Chimpanzee adenoviruses include, but are not limited to AdY25, ChAd3, ChAd19, ChAd25.2, ChAd26, ChAd27, ChAd29, ChAd30, ChAd31, ChAd32, ChAd33, ChAd34, ChAd35, ChAd37, ChAd38, ChAd39, ChAd40, ChAd63, ChAd83, ChAd155, ChAd15, SadV41, sAd4310A, sAd4312, SAdV31, SAdV-A1337, ChAdOx1, ChAdOx2 and ChAd157. Preferably, the simian adenoviral vector of the invention is a ChAd83 or ChAd155 adenovirus vector, most preferably a ChAd155 adenovirus vector.

[0027] Preferably, the adenoviral vector of the invention has a seroprevalence of less than 30%, preferably less than 10% in human subjects and, most preferably, no seroprevalence in human subjects.

[0028] In a preferred embodiment, the simian adenoviral vector of the invention is capable of infecting a mammalian cell.

[0029] In one embodiment, the first and second expression cassettes of the adenoviral vector of the invention comprise transgenes from respiratory syncytial virus (RSV). For example, in one embodiment, one of the expression cassettes comprises an RSV F antigen, and the other expression cassette comprises RSV M and N antigens. In such embodiments, the vector preferably encodes an RSV F0.DELTA.TM antigen (fusion (F) protein deleted of the transmembrane and cytoplasmic regions), and RSV M2-1 (transcription anti-termination) and N (nucleocapsid) antigens.

[0030] The present invention also provides a composition comprising a simian adenoviral vector and a pharmaceutically acceptable excipient.

[0031] In addition, the present invention provides a simian adenoviral vector or composition comprising such an adenoviral vector for use as a medicament, a vaccine, and/or for the therapy or prophylaxis of a disease.

[0032] The invention also provides a method of inducing an immune response in a subject comprising administering the simian adenoviral vector or composition to the subject.

DESCRIPTION OF THE FIGURES

[0033] FIG. 1: Simian adenoviral constructs with a single expression cassette. Inverted terminal repeats (ITR) flank the 3' and 5' ends; E1 is the early gene 1; CMV is the cytomegalovirus promoter; CASI is the CASI promoter, RG is a model antigen, WPRE is the Woodchuck Hepatitis Postranscriptional Regulatory Element, .DELTA.E3 denotes that the early gene 3 is deleted; fiber denotes the adenoviral gene encoding the fiber protein and E4 is the early gene 4. Three different simian adenoviral vectors are shown in FIG. 1. The vector of FIG. 1(i) was constructed by inserting a transgene expression cassette in place of the E3 region of the adenoviral genome ("RC1") (top panel), the vector of FIG. 1(ii) was formed by inserting a transgene expression cassette in the HE1 region, i.e., between the stop codons of the fiber gene and the E4 region ("RC3") (middle panel), and the vector of FIG. 1(iii) was made by inserting a transgene expression cassette in the HE2 region, i.e., between the end of the ITR and the cap site of E4 mRNA ("RC2") (bottom panel).

[0034] FIG. 2A: Production of ChAd155 and ChAd83 with transgene cassette inserted in E3 and HE2 sites (RC1 and RC2 vectors of FIG. 1) in a primary human cell line.

[0035] FIG. 2B: Production of ChAd83 with transgene cassette inserted in E3, HE1 and HE2 (the RC1, RC2 and RC3 vectors of FIG. 1) in a human MRC5 cell line at two and seven days post-infection. Cells were infected at multiplicities of infection of 250 and 1250.

[0036] FIG. 3A: Total viral genome copy number of RC1 and RC2 vector (ChAd155 and ChAd83) of FIG. 1 in a primary human cell line.

[0037] FIG. 3B: Total viral genome copy number of RC1, RC2 and RC3 versions of ChAd83 vector of FIG. 1 in a human MRC5 cell line at two and seven days post-infection. Cells were infected at multiplicities of infection of 250 and 1250.

[0038] FIG. 4: Total viral genome copy number of ChAd155 RC1 and RC2 and ChAd83 RC1 and RC2 vectors of FIG. 1 in a murine cell line (FIG. 4(a), top panel) and in a non-human primate cell line (FIG. 4(b), bottom panel). Cells were infected at multiplicities of infection of 50 and 250.

[0039] FIG. 5: Comparison of the expression levels of ChAd155 RC1 and RC2 vectors expressing a model rabies glycoprotein (RG) transgene in a murine cell line, demonstrated by western blot at two and five days post-infection (FIG. 5(a), top panel). Comparison of the expression levels of ChAd155 RC1 and RC2 vectors with ChAd83 RC1 and RC2 vectors expressing a model rabies glycoprotein (RG) transgene in a murine cell line, demonstrated by western blot at two and five days post-infection (FIG. 5(b), bottom panel). Cells were infected at multiplicities of infection of 50, 250 and 1250.

[0040] FIG. 5(c): Comparison of the expression levels of ChAd83 RC1, RC2 and RC3 vectors expressing a model rabies glycoprotein (RG) transgene in a human MRC5 cell line, demonstrated by western blot at two and seven days post-infection. Cells were infected at multiplicities of infection of 250 and 1250.

[0041] FIG. 6: Another simian adenoviral construct of with a single expression cassette. Inverted terminal repeats (ITR) flank the 3' and 5' ends; human CMV (hCMV) is the cytomegalovirus promoter; F.DELTA.TM (F0DTM) and N.M2-1 are RSV antigens; 2A is a self-cleaving linking sequence; .DELTA.E4 denotes that the early gene 4 is deleted; fiber denotes the adenoviral gene encoding the fiber protein. In the vector of FIG. 6, the transgene expression cassette is inserted in place of the E1 region of the adenoviral genome.

[0042] FIG. 7: A simian adenoviral construct according to the invention with a dual expression cassette. Inverted terminal repeats (ITR) flank the 3' and 5' ends; human CMV (hCMV) is the cytomegalovirus promoter; Enchanced hCMV is the enhanced cytomegalovirus promoter; N-M2-1 and F.DELTA.TM (F0DTM) are the RSV antigens; WPRE is the Woodchuck Hepatitis Postranscriptional Regulatory Element; .DELTA.E3 denotes that the early gene 3 is deleted; fiber denotes the adenoviral gene encoding the fiber protein; and Ad5E4orf6 in a substitute in the early gene 4 (E4) region.

[0043] The vector of FIG. 7 was constructed by inserting a first transgene expression cassette in place of the E1 region of the adenoviral genome, and a second transgene expression cassette in the HE2 region, i.e., downstream of the right ITR.

[0044] FIG. 8: Comparison of the expression levels of vectors expressing F0.DELTA.TM transgene in a MRC5 cell line, demonstrated by western blot at 48 hours and 96 hours post-infection under non-reducing conditions. Cells were infected at multiplicities of infection of 500 and 1250.

[0045] FIG. 9: Comparison of the expression levels of vectors expressing NM2-1 transgene in a MRC5 cell line, demonstrated by western blot at 48 hours post-infection under reducing conditions. Cells were infected at multiplicities of infection of 250 and 1250.

[0046] FIG. 10: Comparison of the immunogencity from ChAd155 vectors expressing the RSV antigen F0.DELTA.TM (F.DELTA.Tm). The data was collected at 4 weeks and 8 weeks after vaccination with a dose of 5.times.10.sup.8 virus particles.

[0047] FIG. 11: Comparison of the immunogencity from ChAd155 vectors expressing the M2 RSV antigen. The data was collected at 3 weeks after vaccination with a dose of either 10.sup.7 or 10.sup.6 virus particles.

[0048] FIGS. 12A and 12B: Illustrate the results from the experiment of Example 9 to investigate the lung T cell responses from ChAd155 vectors. FIG. 12A shows the CD4+ response, and FIG. 12B shows the CD8+ response.

[0049] FIGS. 13A and 13B: Show the results from the experiment of Example 9 to investigate the peripheral T cell responses from ChAd155 vectors. FIG. 13A shows the PBMC CD4+ response, and FIG. 13B shows the PBMC CD8+ response.

[0050] FIGS. 14A and 14B: Also show results from Example 9. FIG. 14A shows the RSV neutralising Ab titres, and FIG. 14B illustrates the ratio of the nAb from day D90 to D0.

[0051] FIGS. 15A. 15B and 15C: Show the results of the immunogencity experiment of Example 10.

[0052] FIGS. 16A and 16B: Western blots obtained using the expression in HeLa cells of the vectors in Example 11.

[0053] FIG. 17: Illustrates the results of the CRPV experiment of Example 12.

[0054] FIG. 18: Shows the results of the HPV dual cassette vector characterisation of Example 13.

ANNOTATION OF THE SEQUENCES

[0055] SEQ ID NO: 1--Polynucleotide sequence encoding wild type ChAd155

[0056] SEQ ID NO: 2--Polynucleotide sequence encoding wild type ChAd83

[0057] SEQ ID NO: 3--Polynucleotide sequence encoding the CASI promoter

[0058] SEQ ID NO: 4--Polynucleotide sequence encoding ChAd155/RSV

[0059] SEQ ID NO: 5--RSV F0.DELTA.TM-N-M2-1 amino acid sequence

[0060] SEQ ID NO: 6--Polynucleotide sequence encoding the enhanced hCMV promoter

[0061] SEQ ID NO: 7--Polynucleotide sequence encoding the hCMV NM2 bghpolyA cassette

[0062] SEQ ID NO: 8--NM2 amino acid (protein) sequence

[0063] SEQ ID NO: 9--Polynucleotide sequence encoding the hCMV F0 WPRE bghpolyA cassette

[0064] SEQ ID NO: 10--F0 amino acid (protein) sequence

[0065] SEQ ID NO: 11--Amino acid sequence of a flexible linker

[0066] SEQ ID NO: 12--Amino acid sequence of a flexible linker

DETAILED DESCRIPTION OF THE INVENTION

[0067] Adenoviruses

[0068] Adenoviruses are nonenveloped icosahedral viruses with a linear double stranded DNA genome of approximately 36 kb. Adenoviruses can transduce numerous cell types of several mammalian species, including both dividing and nondividing cells, without integrating into the genome of the host cell. They have been widely used for gene transfer applications due to their proven safety, ability to achieve highly efficient gene transfer in a variety of target tissues, and large transgene capacity. Human adenoviral vectors are currently used in gene therapy and vaccines but have the drawback of a high worldwide prevalence of pre-existing immunity, following previous exposure to common human adenoviruses.

[0069] Adenoviruses have a characteristic morphology with an icosahedral capsid comprising three major proteins, hexon (II), penton base (III) and a knobbed fiber (IV), along with a number of other minor proteins, VI, VIII, IX, IIIa and IVa2. The hexon accounts for the majority of the structural components of the capsid, which consists of 240 trimeric hexon capsomeres and 12 penton bases. The hexon has three conserved double barrels and the top has three towers, each tower containing a loop from each subunit that forms most of the capsid. The base of the hexon is highly conserved between adenoviral serotypes, while the surface loops are variable. The penton is another adenoviral capsid protein; it forms a pentameric base to which the fiber attaches. The trimeric fiber protein protrudes from the penton base at each of the 12 vertices of the capsid and is a knobbed rod-like structure. The primary role of the fiber protein is to tether the viral capsid to the cell surface via the interaction of the knob region with a cellular receptor. Variations in the flexible shaft, as well as knob regions of fiber, are characteristic of the different adenovral serotypes.

[0070] The adenoviral genome has been well characterized. The linear, double-stranded DNA is associated with the highly basic protein VII and a small peptide pX (also termed mu). Another protein, V, is packaged with this DNA-protein complex and provides a structural link to the capsid via protein VI. There is general conservation in the overall organization of the adenoviral genome with respect to specific open reading frames being similarly positioned, e.g. the location of the E1A, E1B, E2A, E2B, E3, E4, 1_1, L2, L3, L4 and L5 genes of each virus. Each extremity of the adenoviral genome comprises a sequence known as an inverted terminal repeat (ITR), which is necessary for viral replication. The 5' end of the adenoviral genome contains the 5' cis-elements necessary for packaging and replication; i.e., the 5' ITR sequences (which can function as origins of replication) and the native 5' packaging enhancer domains, which contain sequences necessary for packaging linear adenoviral genomes and enhancer elements for the E1 promoter. The 3' end of the adenoviral genome includes 3' cis-elements, including the ITRs, necessary for packaging and encapsidation. The virus also comprises a virus-encoded protease, which is necessary for processing some of the structural proteins required to produce infectious virions.

[0071] The structure of the adenoviral genome is described on the basis of the order in which the viral genes are expressed following host cell transduction. More specifically, the viral genes are referred to as early (E) or late (L) genes according to whether transcription occurs prior to or after onset of DNA replication. In the early phase of transduction, the E1A, E1B, E2A, E2B, E3 and E4 genes of adenovirus are expressed to prepare the host cell for viral replication. The E1 gene is considered a master switch, it acts as a transcription activator and is involved in both early and late gene transcription. E2 is involved in DNA replication; E3 is involved in immune modulation and E4 regulates viral mRNA metabolism. During the late phase of infection, expression of the late genes L1-L5, which encode the structural components of the viral particles, is activated. Late genes are transcribed from the Major Late Promoter (MLP) with alternative splicing.

[0072] HE1 and HE2 sites were identified as potential insertion sites for a transgene since the insertion in these specific points does not interrupt the coding sequences or important regulatory sequences of a chimp adenovirus, such as a Type C or E chimp adenovirus, for example, ChAd155 and ChAd83. The HE1 and HE2 sites can be identified by sequence alignment in any chimp adenovirus. Therefore, cloning of expression cassettes in the HE1 and HE2 sites of the ChAd genomes doesn't impact the virus replication cycle.

[0073] Adenoviral Replication

[0074] Historically, adenovirus vaccine development has focused on defective, non-replicating vectors. They are rendered replication defective by deletion of the E1 region genes, which are essential for replication. Typically, non-essential E3 region genes are also deleted to make room for exogenous transgenes. An expression cassette comprising the transgene under the control of an exogenous promoter is then inserted. These replication-defective viruses are then produced in E1-complementing cells.

[0075] The term "replication-defective" or "replication-incompetent" adenovirus refers to an adenovirus that is incapable of replication because it has been engineered to comprise at least a functional deletion (or "loss-of-function" mutation), i.e. a deletion or mutation which impairs the function of a gene without removing it entirely, e.g. introduction of artificial stop codons, deletion or mutation of active sites or interaction domains, mutation or deletion of a regulatory sequence of a gene etc, or a complete removal of a gene encoding a gene product that is essential for viral replication, such as one or more of the adenoviral genes selected from E1A, E1B, E2A, E2B, E3 and E4 (such as E3 ORF1, E3 ORF2, E3 ORF3, E3 ORF4, E3 ORF5, E3 ORF6, E3 ORF7, E3 ORF8, E3 ORF9, E4 ORF7, E4 ORF6, E4 ORF4, E4 ORF3, E4 ORF2 and/or E4 ORF1). Suitably, E1 and optionally E3 and/or E4 are deleted. If deleted, the aforementioned deleted gene region will suitably not be considered in the alignment when determining percent identity with respect to another sequence.

[0076] Vectors of the Invention

[0077] Viral vectors based on non-human simian adenovirus represent an alternative to the use of human derived vectors for gene therapy and genetic vaccines. Certain adenoviruses isolated from non-human simians are closely related to adenoviruses isolated from humans, as demonstrated by their efficient propagation in cells of human origin. As humans develop little or no immunity to simian adenoviruses, they promise to provide an improved alternative to human adenoviral uses.

[0078] "Low seroprevalence" may mean having a reduced pre-existing neutralizing antibody level as compared to human adenovirus 5 (Ad5). Similarly or alternatively, "low seroprevalence" may mean less than about 30% seroprevalence, less than about 20% seroprevalence, less than about 15% seroprevalence, less than about 10% seroprevalence, less than about 5% seroprevalence, less than about 4% seroprevalence, less than about 3% seroprevalence, less than about 2% seroprevalence, less than about 1% seroprevalence or no detectable seroprevalence. Seroprevalence can be measured as the percentage of individuals having a clinically relevant neutralizing titer (defined as a 50% neutralisation titer>200) using methods as described in Hum. Gene Ther. (2004) 15:293.

[0079] The adenoviral vector of the present invention is derived from a nonhuman simian adenovirus, also referred to as a "simian adenovirus." Numerous adenoviruses have been isolated from nonhuman simians such as chimpanzees, bonobos, rhesus macaques, orangutans and gorillas. Vectors derived from these adenoviruses can induce strong immune responses to transgenes encoded by these vectors. Certain advantages of vectors based on nonhuman simian adenoviruses include a relative lack of cross-neutralizing antibodies to these adenoviruses in the human target population, thus their use overcomes the pre-existing immunity to human adenoviruses. For example, some simian adenoviruses have no cross reactivity with preexisting human neutralizing antibodies and cross-reaction of certain chimpanzee adenoviruses with pre-existing human neutralizing antibodies is only present in 2% of the target population, compared with 35% in the case of certain candidate human adenovirus vectors (Sci. Transl. Med. (2012) 4:1).

[0080] Adenoviral vectors of the invention are derived from a simian adenovirus, e.g., from chimpanzees (Pan troglodytes), bonobos (Pan paniscus), gorillas (Gorilla gorilla) and orangutans (Pongo abelii and Pongo pygnaeus). They include adenoviruses from Group B, Group C, Group D, Group E and Group G. Chimpanzee adenoviruses include, but are not limited to AdY25, ChAd3, ChAd19, ChAd25.2, ChAd26, ChAd27, ChAd29, ChAd30, ChAd31, ChAd32, ChAd33, ChAd34, ChAd35, ChAd37, ChAd38, ChAd39, ChAd40, ChAd63, ChAd83, ChAd155, ChAd15, SadV41 and ChAd157 ChAd3, ChAd19, ChAd25.2, ChAd26, ChAd27, ChAd29, ChAd30, ChAd31, ChAd32, ChAd33, ChAd34, ChAd35, ChAd37, ChAd38, ChAd39, ChAd40, ChAd63, ChAd83, ChAd155, ChAd15, SadV41, sAd4310A, sAd4312, SAdV31, SAdV-A1337, ChAdOx1, ChAdOx2 and ChAd157. Alternatively, adenoviral vectors may be derived from nonhuman simian adenoviruses isolated from bonobos, such as PanAd1, PanAd2, PanAd3, Pan 5, Pan 6, Pan 7 (also referred to as C7) and Pan 9. Vectors may include, in whole or in part, a nucleotide encoding the fiber, penton or hexon of a non-human adenovirus.

[0081] In an embodiment of the adenoviral vectors of the invention, the adenoviral vector has a seroprevalence of less than 30%, less than 20%, less than 10% or less than 5% in human subjects, preferably no seroprevalence in human subjects and more preferably no seroprevalence in human subjects that have not previously been in contact with a chimpanzee adenoviral vector.

[0082] In embodiments of the adenoviral vectors of the invention, the adenoviral DNA is capable of entering a mammalian target cell, i.e. it is infectious. An infectious recombinant adenoviral vector of the invention can be used as a prophylactic or therapeutic vaccine and for gene therapy. Thus, in an embodiment, the recombinant adenoviral vector comprises an endogenous molecule for delivery into a target cell. The target cell is a mammalian cell, e.g. a bovine cell, a canine cell, a caprine cell, a cervine cell, a chimpanzee cell, a chiroptera cell, an equine cell, a feline cell, a human cell, a lupine cell, an ovine cell, a porcine cell, a rodent cell, an ursine cell or a vulpine cell. The endogenous molecule for delivery into a target cell is an expression cassette.

[0083] In an embodiment of the invention, the vector comprises a left ITR region, a deleted E1 region, then a deleted E3 region, and, optionally, additional enhancer elements; these are followed by a fiber region, an E4 region and a right ITR. Translation occurs in the rightward and leftward directions. In this embodiment, the first expression cassette is inserted in the deleted E1 region, and the second expression cassette is insertion in the deleted E3 region. In a further embodiment, the promoters of the two expression cassettes are CMV promoters. In a yet further embodiment, the enhancer element is the Hepatitis B Postranslational Regulatory Element (HPRE) or the Woodchuck Hepatitis Postranslational Regulatory Element (WPRE).

[0084] In one embodiment of the invention, the vector comprises left and right ITR regions; a deleted E1 region; at least a partially deleted E3 region; a fiber region; an E4 region; two expression cassettes, each comprising: a promoter and at least one an antigen of interest and, optionally, one or more enhancer elements. The first expression cassette is inserted in the deleted E1 region, and the second expression cassette is inserted at the HE1 site, i.e., between the stop codons of the fiber gene and an E4 region ("the HE1 site"). The ChAd155 HE1 insertion site is between bp 34611 and 34612 of the wild type ChAd155 sequence. The ChAd83 HE1 insertion site is between bp 33535 and 33536 of the wild type ChAd83 sequence. Translation occurs in the rightward and leftward directions. In a further embodiment, the promoters are CMV promoters. In a preferred embodiment, one promoter is a CMV promoter and the other is a eCMV promoter. In a yet further embodiment, the enhancer element is HPRE or WPRE.

[0085] In a further embodiment, the vector comprises left and right ITR regions; a deleted E1 region; at least a partially deleted E3 region; a fiber region; an E4 region; two expression cassettes, each comprising: a promoter, at least one antigen of interest and, optionally, one or more enhancer elements. The first expression cassette is inserted in the deleted E1 region, and the second expression cassette is inserted at the HE2 site, i.e., between the end of the left ITR and the cap site of the E4 mRNA ("the HE2 site"). The ChAd155 HE2 insertion site is between bp 37662 and 37663 of the wild type ChAd155 sequence. The ChAd83 HE2 insertion site is between bp 36387 and 36388 of the wild type ChAd83 sequence. Translation occurs in the rightward and leftward directions. In a further embodiment, the promoters are CMV promoters. In a preferred embodiment, one promoter is a CMV promoter and the other is a eCMV promoter. In a yet further embodiment, the enhancer element is HPRE or WPRE (the enhancer element increases expression of the transgene).

[0086] The HE1 and HE2 sites were identified as insertion sites for a transgene, as the insertion in these specific points does not interrupt the coding sequences or regulatory sequences of ChAd155 and ChAd83. Therefore, inserting expression cassettes in the HE1 or HE2 sites of the ChAd genome does not affect the viral replication cycle.

[0087] In an embodiment of the invention, the vector is a functional or an immunogenic derivative of an adenoviral vector. By "derivative of an adenoviral vector" is meant a modified version of the vector, e.g., one or more nucleotides of the vector are deleted, inserted, modified or substituted.

[0088] Regulatory Elements

[0089] Regulatory elements, i.e., expression control sequences, include appropriate transcription initiation, termination, promoter and enhancer sequences; efficient RNA processing signals such as splicing and polyadenylation (poly A) signals including rabbit beta-globin polyA; tetracycline regulatable systems, microRNAs, posttranscriptional regulatory elements (e.g., WPRE, posttranscriptional regulatory element of woodchuck hepatitis virus); sequences that stabilize cytoplasmic mRNA; sequences that enhance translation efficiency (e.g., Kozak consensus sequence); sequences that enhance protein stability; and when desired, sequences that enhance secretion of an encoded product.

[0090] A "promoter" is a nucleotide sequence that permits the binding of RNA polymerase and directs the transcription of a gene. Typically, a promoter is located in a non-coding region of a gene, proximal to the transcriptional start site. Sequence elements within promoters that function in the initiation of transcription are often characterized by consensus nucleotide sequences. Examples of promoters include, but are not limited to, promoters from bacteria, yeast, plants, viruses, and mammals, including simians and humans. A great number of expression control sequences, including promoters which are internal, native, constitutive, inducible and/or tissue-specific, are known in the art and may be utilized.

[0091] Promoters of the invention will typically be heterologous promoters. Promoters of the invention can be constitutive.

[0092] Examples of promoters include, but are not limited to, promoters from bacteria, yeast, plants, viruses, and mammals (including humans).

[0093] Examples of promoters include, without limitation, the TBG promoter, the retroviral Rous sarcoma virus LTR promoter (optionally with the enhancer), the cytomegalovirus (CMV) promoter (optionally with the CMV enhancer, see, e.g., Boshart et al, Cell, 41:521-530 (1985)), the CASI promoter, the SV40 promoter, the dihydrofolate reductase promoter, the .beta.-actin promoter, the phosphoglycerol kinase (PGK) promoter, and the EF1a promoter (Invitrogen).

[0094] Suitable promoters include the cytomegalovirus (CMV) promoter and the CASI promoter. The CMV promoter is strong and ubiquitously active. It has the ability to drive high levels of transgene expression in many tissue types and is well known in the art. The CMV promoter can be used in vectors of the invention, either with or without a CMV enhancer.

[0095] The CASI promoter is a synthetic promoter described as a combination of the CMV enhancer, the chicken beta-actin promoter, and a splice donor and splice acceptor flanking the ubiquitin (UBC) enhancer (U.S. Pat. No. 8,865,881).

[0096] In some embodiments, the CASI promoter can include a nucleic acid sequence having at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more, sequence identity to SEQ ID NO: 3. In some embodiments, the promoter comprises or consists of a nucleic acid sequence of SEQ ID NO: 3.

[0097] In some embodiments, the enhanced hCMV promoter can include a nucleic acid sequence having at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or more, sequence identity to SEQ ID NO: 6. In some embodiments, the promoter comprises or consists of a nucleic acid sequence of SEQ ID NO: 6.

[0098] Optionally, vectors carrying transgenes encoding therapeutically useful or immunogenic products may also include selectable markers or reporter genes. The reporter gene may be chosen from those known in the art. Suitable reporter genes include, but are not limited to enhanced green fluorescent protein, red fluorescent protein, luciferase and secreted embryonic alkaline phosphatase (seAP), which may include sequences encoding geneticin, hygromicin or purimycin resistance, among others. Such selectable reporters or marker genes (which may or may not be located outside the viral genome to be packaged into a viral particle) can be used to signal the presence of the plasmids in bacterial cells, such as ampicillin resistance. Other components of the vector may include an origin of replication.

[0099] A "posttranscriptional regulatory element," as used herein, is a DNA sequence that, when transcribed, enhances the expression of the transgene(s) or fragments thereof that are delivered by viral vectors of the invention. Postranscriptional regulatory elements include, but are not limited to the Hepatitis B Virus Postranscriptional Regulatory Element (HPRE) and the Woodchuck Hepatitis Postranscriptional Regulatory Element (WPRE). The WPRE is a tripartite cis-acting element that has been demonstrated to enhance transgene expression driven by certain, but not all promoters.

[0100] In embodiments of the invention, a ChAd155 vector may comprise one or more of a promoter, an enhancer, and a reporter gene. For example, vectors of the invention may comprise ChAd155-enhanced hCMV-SeAP, ChAd155-CASI-seAP and ChAd155-hCMV-seAP, optionally with a tetracycline on/off transcriptional control and ChAd155-CMV-hFerL-chEF1-seAP with a tetracycline on/off transcriptional control.

[0101] In embodiments of the invention, a ChAd83 vector may comprise one or more of a promoter, an enhancer, and a reporter gene. For example, vectors of the invention may comprise ChAd155-enhanced hCMV-SeAP, ChAd83-enhanced hCMV-SeAP, ChAd155-CASI-seAP and ChAd83-hCMV-seAP, optionally with a tetracycline on/off transcriptional control and ChAd83-CMV-hFerL-chEF1-seAP with a tetracycline on/off transcriptional control.

[0102] Vectors of the invention are generated using techniques provided herein, in conjunction with techniques known to those of skill in the art. Such techniques include conventional cloning techniques of cDNA such as those described in texts, use of overlapping oligonucleotide sequences of the adenovirus genomes, polymerase chain reaction, and any suitable method which provides the desired nucleotide sequence.

[0103] Transgenes

[0104] A "transgene" is a nucleic acid sequence, heterologous to the vector sequences flanking the transgene, which encodes a polypeptide of interest. The nucleic acid coding sequence is operatively linked to regulatory components in a manner which permits transgene transcription, translation, and/or expression in a host cell. In embodiments of the invention, the vectors express transgenes at a therapeutic or a prophylactic level. A "functional derivative" of a transgenic polypeptide is a modified version of a polypeptide, e.g., wherein one or more amino acids are deleted, inserted, modified or substituted.

[0105] The transgene may be used for prophylaxis or treatment, e.g., as a vaccine for inducing an immune response, to correct genetic deficiencies by correcting or replacing a defective or missing gene, or as a cancer therapeutic. As used herein, induction of an immune response refers to the ability of a protein to induce a T cell and/or a humoral antibody immune response to the protein.

[0106] The immune response elicited by the transgene may be an antigen specific B cell response, which produces neutralizing antibodies. The elicited immune response may be an antigen specific T cell response, which may be a systemic and/or a local response. The antigen specific T cell response may comprise a CD4+ T cell response, such as a response involving CD4+ T cells expressing cytokines, e.g. interferon gamma (IFN gamma), tumor necrosis factor alpha (TNF alpha) and/or interleukin 2 (IL2). Alternatively, or additionally, the antigen specific T cell response comprises a CD8+ T cell response, such as a response involving CD8+ T cells expressing cytokines, e.g., IFN gamma, TNF alpha and/or IL2.

[0107] The composition of the transgene sequence will depend upon the use to which the resulting vector will be put. In an embodiment, the transgene is a sequence encoding a product which is useful in biology and medicine, such as a prophylactic transgene, a therapeutic transgene or an immunogenic transgene, e.g., protein or RNA. Protein transgenes include antigens. Antigenic transgenes of the invention induce an immunogenic response to a disease causing organism.

[0108] Transgenes of the invention include, but are not limited to, rabies virus antigens, e.g., rabies glycoprotein (RG), respiratory syncytial virus (RSV) antigens, human immunodeficiency virus (HIV) antigens, or fragments thereof.

[0109] As a result of the redundancy in the genetic code, a polypeptide can be encoded by a variety of different nucleic acid sequences. Coding is biased to use some synonymous codons, i.e., codons that encode the same amino acid, more than others. By "codon optimized," it is meant that modifications in the codon composition of a recombinant nucleic acid are made without altering the amino acid sequence. Codon optimization has been used to improve mRNA expression in different organisms by using organism-specific codon-usage frequencies.

[0110] In addition to, and independently from, codon bias, some synonymous codon pairs are used more frequently than others. This codon pair bias means that some codon pairs are overrepresented and others are underrepresented. Codon pair deoptimization has been used to reduce viral virulence. For example, it has been reported that polioviruses modified to contain underrepresented codon pairs demonstrated decreased translation efficiency and were attenuated compared to wild type poliovirus (Science (2008) 320:1784). Engineering a synthetic attenuated virus by codon pair deoptimization can produce viruses that encode the same amino acid sequences as wild type but use different pairwise arrangements of synonymous codons. Viruses attenuated by codon pair deoptimization generated up to 1000-fold fewer plaques compared to wild type, produced fewer viral particles and required about 100 times as many viral particles to form a plaque.

[0111] In contrast, polioviruses modified to contain codon pairs that are overrepresented in the human genome acted in a manner similar to wild type RNA and generated plaques identical in size to wild type RNA (Coleman et al. (2008) Science 320:1784). This occurred despite the fact that the virus with overrepresented codon pairs contained a similar number of mutations as the virus with underrepresented codon pairs and demonstrated enhanced translation compared to wild type. This observation suggests that codon pair optimized constructs would be expected to act in a manner similar to their non-codon pair optimized counterparts and would not be expected to provide a functional advantage. Without wishing to be constrained by theory, this may be because natural evolution has optimized codon pairing.

[0112] A construct of the invention may comprise a codon optimized nucleic acid sequence. Alternatively or additionally, a vector of the invention comprises a codon optimized sequence of a transgene or an immunogenic derivative or fragment thereof. A construct of the invention may comprise a codon pair optimized nucleic acid sequence. Alternatively or additionally, a vector of the invention comprises or consists of a codon pair optimized sequence of a transgene or an immunogenic derivative or fragment thereof.

[0113] Respiratory Syncytial Virus (RSV) Transgenes

[0114] In one embodiment, the present invention provides the use of a recombinant simian-derived adenoviral vector comprising two expression cassettes, wherein each expression cassette comprises an immunogenic transgene derived from human respiratory syncytial virus (RSV), in the treatment or prophylaxis of RSV infection. In one embodiment, the recombinant simian-derived adenoviral vector of the present invention comprises an RSV F antigen in one of the expression cassettes, and another RSV viral antigen in the other expression cassette. Suitable antigens are discussed further below. In one embodiment, the recombinant simian-derived adenoviral vector comprises RSV M and N antigens in the second expression cassette. In such embodiments, the vector preferably encodes an RSV F0.DELTA.TM antigen (fusion (F) protein deleted of the transmembrane and cytoplasmic regions), and RSV M2-1 (transcription anti-termination) and N (nucleocapsid) antigens.

[0115] Infection with RSV does not confer full protective immunity. Infection in infancy is followed by symptomatic RSV re-infections which continue throughout adulthood. These re-infections generally go undiagnosed because they usually present as common acute upper respiratory tract infections. In more vulnerable persons (e.g., immunocompromised adults or elderly), re-infections can however also lead to severe disease. Both arms of the immune system (humoral and cellular immunity) are involved in protection from severe disease [Guvenel A K, Chiu C and Openshaw P J. Current concepts and progress in RSV vaccine development. Expert Rev Vaccines. 2014; 13(3): 333-44.].

[0116] The humoral immune response is capable of neutralizing the virus and inhibiting viral replication, thereby playing a major role in protection against lower respiratory RSV infection and severe disease [Piedra P A, Jewell A M, Cron S G, et al., Correlates of immunity to respiratory syncytial virus (RSV) associated-hospitalization: establishment of minimum protective threshold levels of serum neutralizing antibodies. Vaccine. 2003; 21(24): 3479-82.]. Passive immunization, in the form of Immunoglobulin G (IgG) RSV-neutralizing monoclonal antibodies (Synagis) given prophylactically, has been shown to prevent RSV disease to some extent in premature infants and newborns with bronchopulmonary dysplasia or underlying cardiopulmonary disease [Cardenas S, Auais A and Piedimonte G. Palivizumab in the prophylaxis of respiratory syncytial virus infection. Expert Rev Anti Infect Ther. 2005; 3(5): 719-26].

[0117] T cells are also involved in the control of RSV disease. Lethal RSV infections have been described in patients with low CD8 T cells counts, as in the case of severe combined immunodeficiency, bone marrow and lung transplant recipients [Hertz, 1989]. The histopathology of fatal cases of RSV infection of newborns shows that there is a relative paucity of CD8 T cells in the lung infiltrate [Welliver T P, Garofalo R P, Hosakote Y, et al., Severe human lower respiratory tract illness caused by respiratory syncytial virus and influenza virus is characterized by the absence of pulmonary cytotoxic lymphocyte responses. J Infect Dis. 2007. 195(8): 1126-36.]. Moreover, the presence of CD8 T cells producing Interferon-gamma (IFN-.gamma.) has been associated with diminished Th2 responses and reduced eosinophilia in animal models of RSV [Castilow E M and Varga S M. Overcoming T cell-mediated immunopathology to achieve safe RSV vaccination. Future Virol. 2008; 3(5): 445-454; Stevens W W, Sun J, Castillo J P, et al., Pulmonary eosinophilia is attenuated by early responding CD8(+) memory T cells in a murine model of RSV vaccine-enhanced disease. Viral Immunol. 2009; 22(4): 243-51].

[0118] Suitable antigens of RSV which are useful as immunogens to immunize a human or non-human animal can be selected from: the fusion protein (F), the attachment protein (G), the matrix protein (M2) and the nucleoprotein (N). The term "F protein" or "fusion protein" or "F protein polypeptide" or "fusion protein polypeptide" refers to a polypeptide or protein having all or part of an amino acid sequence of an RSV Fusion protein polypeptide. Similarly, the term "G protein" or "G protein polypeptide" refers to a polypeptide or protein having all or part of an amino acid sequence of an RSV Attachment protein polypeptide. The term "M protein" or "matrix protein" or "M protein polypeptide" refers to a polypeptide or protein having all or part of an amino acid sequence of an RSV Matrix protein and may include either or both of the M2-1 (which may be written herein as M2.1) and M2-2 gene products. Likewise, the term "N protein" or "Nucleocapsid protein" or "N protein polypeptide" refers to a polypeptide or protein having all or part of an amino acid sequence of an RSV Nucleoprotein.

[0119] Two groups of human RSV strains have been described, the A and B groups, based mainly on differences in the antigenicity of the G glycoprotein. Numerous strains of RSV have been isolated to date, any of which are suitable in the context of the antigens of the immunogenic combinations disclosed herein. Exemplary strains indicated by GenBank and/or EMBL Accession number can be found in US published application number 2010/0203071 (WO2008114149), which is incorporated herein by reference for the purpose of disclosing the nucleic acid and polypeptide sequences of RSV F and G proteins suitable for use in present invention. In an embodiment, the RSV F protein can be an ectodomain of an RSV F Protein (F0.DELTA.TM).

[0120] Exemplary M and N protein nucleic acids and protein sequences can be found, e.g., in US published application number 2014/0141042 (WO2012/089833), which are incorporated herein for purpose of disclosing the nucleic acid and polypeptide sequences of RSV M and N proteins suitable for use in present invention.

[0121] Suitably, for use with in present invention, transgene nucleic acids encode an RSV F antigen and RSV, M and N antigens. More specifically, the nucleic acids encode an RSV F0.DELTA.TM antigen (fusion (F) protein deleted of the transmembrane and cytoplasmic regions), and RSV M2-1 (transcription anti-termination) and N (nucleocapsid) antigens.

[0122] Fusion (F) Protein Deleted of the Transmembrane and Cytoplasmic Regions (F0.DELTA.TM)

[0123] The RSV F protein is a major surface antigen and mediates viral fusion to target cells. The F protein is an antigen which is highly conserved among RSV subgroups and strains. The F protein is a target for neutralizing antibodies, including the prophylactic RSV-neutralizing monoclonal antibody Synagis. Deletion of the transmembrane region and cytoplasmic tail permits secretion of the F0.DELTA.TM protein. Neutralizing antibodies including Synagis, that recognize this soluble form of the F protein, inhibit RSV infectivity in vitro [Magro M, Andreu D, Gomez-Puertas P, et al., Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein. J Virol. 2010; 84(16): 7970-82].

[0124] Nucleocapsid (N) Protein

[0125] The N protein is an internal (non-exposed) antigen, highly conserved between RSV strains and known to be a source of many T cell epitopes. The N protein is essential for the replication and transcription of the RSV genome. The primary function of the N protein is to encapsulate the virus genome for the purposes of RNA transcription, replication and packaging and protects it from ribonucleases.

[0126] Transcription Anti-Termination (M2-1) Protein

[0127] The M2-1 protein is a transcription anti-termination factor that is important for the efficient synthesis of full-length messenger RNAs (mRNAs) as well as for the synthesis of polycistronic readthrough mRNAs, which are characteristic of non-segmented negative-strand RNA viruses. M2-1 is an internal (non-exposed) antigen, which is highly conserved between RSV strains and known to be a source of many T cell epitopes.

[0128] N-M2-1 Fusion Protein

[0129] A polynucleotide encoding a linker is positioned between the polynucleotide encoding an RSV N antigen, or fragment thereof, and the polynucleotide encoding an RSV M2.1 antigen, or fragment thereof. Thus, in certain preferred examples, an expression cassette contains a transgene which encodes a fused RSV viral protein N-linker-M2.1 It is preferred that the linker is a flexible linker, preferably a flexible linker comprising an amino acid sequence according to SEQ ID NO: 11 (Gly-Gly-Gly-Ser-Gly-Gly-Gly) or SEQ ID NO: 12 (Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly).

[0130] Papilloma (PV) Transgenes

[0131] In one embodiment, the present invention provides the use of a recombinant simian-derived adenoviral vector comprising two expression cassettes, wherein each expression cassette comprises an immunogenic transgene derived from a papilloma virus (PV), in the treatment or prophylaxis of a papilloma virus induced disease. Suitably, the recombinant simian-derived adenoviral vector of the present invention comprises a modified papilloma virus E1 antigen in one of the expression cassettes, and a modified papilloma virus E2 antigen in the other expression cassette.

[0132] Human Papillomavirus (HPV) are small DNA viruses that infect mucosal and/or cutaneous skin and cause multiple disease conditions, including cervical neoplasia, cervical cancer, and other anogenital cancers. There are over 40 types of HPV known to infect the anogenital tract of humans and about 15 high-risk HPV genotypes are causally associated with human cervical cancers. A majority of HPV infections of the cervical epithelium are subclinal and self-resolving within a two years period. However, persistant infection with high risk HPV types may cause lesions and progress to invasive cancer.

[0133] Suitable antigens of HPV which are useful as immunogens are described in WO2018060288 and include in particular HPV E1 and E2proteins.

[0134] Rabies (RG) Transgenes

[0135] Lyssavirus is an enveloped, single stranded RNA virus in the Rhabdoviridae family. Members of the Lyssavirus genus cause rabies and have the highest fatality rate of all known human viral pathogens. Rabies is transmitted via the saliva of infected mammals. A neurotropic virus, it enters the nervous system of its host, causing an encephalomyelitis that is almost invariably fatal. Currently there are about 60,000 rabies deaths worldwide yearly, mostly caused by dog bites in developing countries in Asia and Africa and by wildlife and bats in North America.

[0136] Rabies presents either in a furious or a paralytic form. The incubation period varies between about five days and several years but is typically between about 20 and 90 days. Clinical illness most often starts with prodromal complaints of malaise, anorexia, fatigue, headache and fever followed by pain or parathesia at the site of exposure. Anxiety, agitation or irritability may be prominent during this period, followed by hyperactivity, disorientation, seizures, hydrophobia, hypersalivation and, eventually, paralysis, coma and death.

[0137] Rabies antigens may be derived from the rabies viral glycoprotein (RG). For example, rabies glycoprotein may be used as a model antigen.

[0138] Delivery of Adenoviral Vectors

[0139] In some embodiments, the recombinant adenoviral vector of the invention is administered to a subject by epicutaneous administration, intradermal administration, intramuscular injection, intraperitoneal injection, intravenous injection, nasal administration, oral administration, rectal administration, subcutaneous injection, transdermal administration or intravaginal administration.

[0140] In an embodiment of the invention, the vectors can be administered intramuscularly (IM), i.e., injection directly into muscle. Muscles are well vascularized and the uptake is typically rapid.

[0141] Adjuvants

[0142] Approaches to establishing strong and lasting immunity to specific pathogens include addition of adjuvants to vaccines. By "adjuvant" is meant an agent that augments, stimulates, activates, potentiates or modulates the immune response to an active ingredient of the composition. The adjuvant effect may occur at the cellular or humoral level, or both. Adjuvants stimulate the response of the immune system to the actual antigen but have no immunological effect themselves. Alternatively or additionally, adjuvented compositions of the invention may comprise one or more immunostimulants. By "immunostimulant" it is meant an agent that induces a general, temporary increase in a subject's immune response, whether administered with the antigen or separately.

[0143] A composition of the invention may be administered with or without an adjuvant. Alternatively, or additionally, the composition may comprise, or be administered in conjunction with, one or more adjuvants (e.g. vaccine adjuvants), in particular the composition comprises an immunologically effective amount of a vector of the invention encoding a transgene.

[0144] Methods of Use/Uses

[0145] Methods are provided for inducing an immune response against a disease caused by a pathogen in a subject in need thereof comprising a step of administering an immunologically effective amount of a construct or composition as disclosed herein. In some embodiments are provided the use of the constructs or compositions disclosed herein for inducing an immune response to a transgenic antigen in a subject in need thereof. Vectors of the invention may be applied for the prophylaxis, treatment or amelioration of diseases due to infection.

[0146] Methods of the invention include the use of a vector of the invention in medicine. They include the use of a vector of the invention for the treatment of a disease caused by a pathogen. A vector of the invention can be used in the manufacture of a medicament for treating a disease caused by a pathogen. A vector of the invention can be used in the manufacture of a medicament for the prevention or treatment of a disease, for example, a disease caused by respiratory syncytial virus (RSV).

[0147] Effective immunization with adenoviral vectors depends on the intrinsic immnomodulatory capability of the adenoviral vector backbone. Immunologically less potent adenoviruses induce less antigen expression. Effective immunization also depends on the ability of the promoter to drive strong and sustained transgene expression. For example, adenoviral vectors driven by the cytomegalovirus immediate-early (CMV-IE) promoter do not sustain long-term transgene expression because they induce cytokines that dampen expression.

[0148] By "subject" is intended a vertebrate, such as a mammal e.g. a human or a veterinary mammal. In some embodiments the subject is human.

[0149] General

[0150] Vectors of the invention are generated using techniques and sequences provided herein, in conjunction with techniques known to those of skill in the art. Such techniques include conventional cloning techniques of cDNA such as those described in texts, use of overlapping oligonucleotide sequences of the adenovirus genomes, polymerase chain reaction, and any suitable method which provides the desired nucleotide sequence.

[0151] Unless otherwise explained, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. The singular terms "a," "an," and "the" include plural referents unless context clearly indicates otherwise. Similarly, the word or is intended to include "and" unless the context clearly indicates otherwise. The term "plurality" refers to two or more. Additionally, numerical limitations given with respect to concentrations or levels of a substance, such as solution component concentrations or ratios thereof, and reaction conditions such as temperatures, pressures and cycle times are intended to be approximate. The term "about" used herein is intended to mean the amount.+-.10%.

[0152] The present invention will now be further described by means of the following non-limiting examples.

EXAMPLES

Example 1: Construction of Chimpanzee Adenoviruses with a Single Expression Cassette

[0153] Wild type chimpanzee adenoviruses type 155 (ChAd155) (WO 2016/198621) and type 83 (ChAd83) (WO 2010/086189) were isolated from healthy chimpanzees using standard procedures and were constructed as described in Sci Transl Med (2012) 4:1 and WO 2010/086189.

[0154] In Example 1, the ChAd155 and ChAd 83 vectors were each constructed by inserting a single transgene expression cassette. The expression cassette components used either the classical human CMV promoter or the CASI promoter, rabies glycoprotein as a model antigen and, optionally, a WPRE enhancer. Three different insertion sites were tested for the transgene cassette:

[0155] (i) replacing the E3 region with the transgene cassette,

[0156] (ii) inserting the transgene cassette between the fiber and the E4 region (site HE1), and

[0157] (iii) inserting the transgene cassette downstream of the right ITR (site HE2).

[0158] This numbering of these insertion sites corresponds to the illustrations of FIG. 1 where:

[0159] (i) the top panel illustrates the RC1 vector, in which a transgene cassette replaced the E3 region,

[0160] (ii) the middle panel illustrates the RC3 vector, in which a transgene cassette is inserted between the stop codons of the fiber gene and the E4 region (site HE1), and

[0161] (iii) the bottom panel illustrates the RC2 vector, in which a transgene cassette is inserted downstream of the right ITR (site HE2).

[0162] In the vectors shown in Example 1, the E1 region remains intact in all configurations.

[0163] The transgene was inserted by homologous recombination techniques in the following positions of the SEQ ID NO: 1 and of the SEQ ID NO: 2:

[0164] HE1 ChAd155: insertion site between bp 34611 and 34612 of SEQ ID NO: 1;

[0165] HE2 ChAd155: insertion site between bp 37662 and 37663 of SEQ ID NO: 1;

[0166] HE1 ChAd83: insertion site between bp 33535 and 33536 of SEQ ID NO: 2;

[0167] HE2 ChAd83: insertion site between bp 36387 and 36388 of SEQ ID NO: 2.

[0168] When the transgene cassette was inserted in site HE1, ChAd155 failed to replicate. However, insertion of a transgene cassette into the HE1 site of ChAd83 produced a viable vector.

Example 2: Virus Production, Vector Titer and Expression of Vectors of Example 1

[0169] To identify an animal model in which to evaluate vector replication, a type C adenovirus ChAd155 RC2 and a type E adenovirus ChAd83 RC2 vectors of Example 1 were assessed for their ability to replicate, measured by vector titer and genome copy number, in cells of various animal origins. The results are shown in Table 1.

TABLE-US-00001 TABLE 1 Replication and Expression of RC2 ChAd155 and RC2 ChAd83 of Example 1 Cell line: Vector Genome Expression Species Vector Titer Copy Day 2 Day 7 MRC5: ChAd155 +++ +++ ++ ++++ Human ChAd83 +++++ +++++ +++ +++++ PK15: ChAd155 +++++ +++++ NA NA Swine ChAd83 +++ ++++ NA NA NMuLi: ChAd155 ++ +++ +++ +++ Mouse ChAd83 ND + ++ ++ Vero: ChAd155 ++ ++++ +++ +++ Non-human ChAd83 ND + + + primate ND = not detected; NA = not available

[0170] As shown in Table 1, human MRC5 cells and swine PK15 cells produced high vector titers and high genome copy numbers of both ChAd155 and ChAd83. Murine NMuLi and non-human primate Vero cells also produced RC ChAd155 but to a lesser extent than the human or swine cells. RC ChAd83 failed to grow well in murine NMuLi cells and, surprisingly, in non-human primate Vero cells.

[0171] Human MRC5, mouse NMuLi and non-human primate Vero cells supported the expression of RC ChAd155 through day 7. Human MRC5 cells supported the expression of RC ChAd83 through day 7, as did mouse NMuLi and non-human primate Vero cells, but to a lesser extent than the human cells.

[0172] Virus Production

[0173] FIG. 2A shows the amount of virus produced by human primary MRC5 cells infected with either ChAd155 or ChAd83, each comprising either the RC1 or RC2 vector construction of Example 1. The cells were harvested seven days post-infection and the vector titer was evaluated in cell lysates obtained following three freeze-thaw cycles. Vector titers were measured by quantitative PCR (QPCR) analysis with primers designed for the respective promoter regions. The multiplicity of infection (moi) was 1250 virus particles per cell. The virus production is indicated in the number of virus particles per cell (vp/cell) above the bars.

[0174] Human MRC5 cells supported production of ChAd155 comprising either RC1 (2.17.times.10.sup.3 vp/cell) or RC2 (4.40.times.10.sup.3 vp/cell) and also supported production of ChAd83 comprising either RC1 (1.18.times.10.sup.4 vp/cell) or RC2 (1.06.times.10.sup.5 vp/cell). As shown in FIG. 2A, ChAd83 was produced at a higher level than ChAd155; the ChAd83 vector comprising RC2 was the most robust of the four viral/vector combinations.

[0175] FIG. 2B shows the amount of virus produced by human primary MRC5 cells infected with ChAd83 comprising the RC1, RC2 or RC3 vector construction of Example 1. The cells were harvested two and seven days post-infection. As with FIG. 2A, vector titers were measured by quantitative PCR (QPCR) analysis with primers designed for the respective promoter regions. The multiplicity of infection (moi) was 250 or 1250 virus particles per cell. The virus production is indicated in the number of virus particles per cell (vp/cell) above the bars.

[0176] Human MRC5 cells supported production of ChAd83 comprising RC1, RC2 or RC3. As shown in FIG. 2B, there was higher virus production for the RC2 and RC3 ChAd83 vectors than for the RC1 vector. There was also higher virus production for the ChAd83 RC2 HE2 vector than the RC3 HE1 vector.

[0177] Vector Genome Copy Number

[0178] After infection, the vector is replicated in the cell and the vector genome copy number can be measured by QPCR. Vector DNA replication can occur even in cells not fully permissive for viral replication and propagation. QPCR of vector DNA provides a measure of vector replication within the infected cell, independently of the ability of the virus to complete the replication cycle and be released as mature viral progeny. Vector replication can thus be quantified in animal species, tissue types and cell types which are not permissive for ChAd virus replication or propagation.

[0179] Vector genome copy number was measured in parallel with vector titer and the results shown in FIG. 3A and FIG. 3B.

[0180] As with the virus production shown in FIG. 2A, Human MRC5 cells were infected with either ChAd155 or ChAd83, each comprising either the RC1 or RC2 vector construction of Example 1. The cells were harvested seven days post-infection, the total DNA extracted, the viral genome quantified by QPCR and the results expressed as vector genome copy per cell. The multiplicity of infection (moi) was 250 virus particles per cell and the numbers of virus particles per cell are indicated above the bars denoting viral genome copies per cell. The copy number is directly proportional to the level of transgene expression.

[0181] As shown in FIG. 3A, the amount of viral DNA replication of RC1 (6.21.times.10.sup.3 vp/cell) and RC2 (6.71.times.10.sup.3 vp/cell) by ChAd155 was similar. ChAd83 produced more RC1 (2.76.times.10.sup.4 vp/cell) and RC2 (9.19.times.10.sup.4 vp/cell) viral DNA than ChAd155. The highest level of viral DNA replication was observed by ChAd83 RC2.

[0182] As with the virus production shown in FIG. 2B, Human MRC5 cells were infected with ChAd83, comprising the RC1, RC2 or RC3 vector construction of Example 1. The cells were harvested at two and seven days post-infection, the total DNA extracted, the viral genome quantified by QPCR and the results expressed as vector genome copy per cell. The multiplicity of infection (moi) was 250 or 1250 virus particles per cell and the numbers of virus particles per cell are indicated above the bars denoting viral genome copies per cell. The copy number is directly proportional to the level of transgene expression.

[0183] As shown in FIG. 3B, the amount of viral DNA replication was higher for the RC2 and RC3 ChAd83 vectors than for the RC1 vector. There was comparable viral DNA replication between the RC2 and RC3 ChAd83 vectors.

Example 3: Adenoviral Genome Copy Number of Vectors of Example 1

[0184] The efficiency of the replication competent adenoviral vectors with the constructs of Example 1, expressed as vector copies per cell, was evaluated in cell cultures derived from both mice and non-human primates.

[0185] FIG. 4(a) shows the genome copy number of replication competent vectors grown in murine hepatic NMuLi cells grown in monolayers and infected with ChAd155 RC1, ChAd155 RC2, ChAd83 RC1 or ChAd83 RC2 at a multiplicity of infection of 250 virus particles per cell. Total DNA was extracted at five days post-infection and the vector replication was measured by QPCR using primers annealing to the vector's promoter region.

[0186] The results, expressed as vector copies per cell, are shown in FIG. 4(a). ChAd155 amplified both the RC1 and RC2 vector with high efficiency in NMuLi cells. ChAd155 replicated the RC1 (1.73.times.10.sup.4) and RC2 (1.92.times.10.sup.4) vectors to approximately the same degree. ChAd83 was less efficient than ChAd155 in replicating the RC1 and RC2 vectors. ChAd83 replicated the vector DNA only in small amounts in the murine cells. RC1 vector replicated at a level of 5.47.times.10.sup.2 copies per cell and the RC2 vector at a level of 6.74.times.10.sup.2 copies per cell.

[0187] Non-human primate Vero cells were also grown in monolayers and infected with ChAd155 RC1, ChAd155 RC2, ChAd83 RC1 or ChAd83 RC2 (FIG. 4(b)). Two different multiplicities of infection were used: 50 and 250 virus particles per cell. Total DNA was extracted at five days post-infection and the vector replication was measured by QPCR using primers annealing to the vector's promoter region.

[0188] The results, expressed as vector copies per cell, are shown in FIG. 4(b). The Vero primate cell line was permissive for ChAd155 RC1 (3.71.times.10.sup.3 copies per cell at an moi of 50 and 4.93.times.10.sup.4copies per cell at an moi of 250) and ChAd155 RC2 (8.15.times.10.sup.3 copies per cell at an moi of 50 and 7.05.times.10.sup.4 copies per cell at an moi of 250). The Vero primate cell line was poorly, if at all, permissive for ChAd83 RC1 or ChAd83 RC2. No ChAd83 RC1 or ChAd83 RC2 vectors were detected to be expressed from Vero cells at an moi of 50. At an moi of 250, ChAd83 replicated the RC1 vector at a level of 1.13.times.10.sup.2 copies per cell and the RC2 vector at a level of 1.29.times.10.sup.3 copies per cell.

Example 4: Transgene Expression from Murine and Non-Human Primate Cells of Vectors of Example 1

[0189] Western blot analysis was performed to compare the level of transgene expression by ChAd155 RC1 and ChAd155 RC2 in murine NMuLi cells (FIG. 5(a)). The cells were infected with ChAd155 RC1 or ChAd155 RC2 at a multiplicity of infection of 50, 250 or 1250 viral particles per cell. The cells were harvested at two and five days post infection, extracts prepared using standard methods and an equivalent amount of total cell extract loaded onto SDS-PAGE gels. Following electrophoretic separation, the proteins were transferred onto nitrocellulose membranes, which were then probed with a commercially available monoclonal antibody to the rabies glycoprotein transgene.

[0190] FIG. 5(a) demonstrates that both ChAd155 RC1 and ChAd155 RC2 express a transgene in murine NMuLi cells. Expression was observed at both two and five days post infection, indicated by the band of about 51 kDa, which corresponds to the expected molecular weight of the rabies glycoprotein (RG). The ChAd155 RC2 vector produced a higher level of transgene expression than the ChAd155 RC1 vector at both two and five days post-infection.

[0191] Western blot analysis was then performed to compare the level of transgene expression by ChAd155 RC1, ChAd155 RC2, ChAd83 RC1 and ChAd83 RC2 in murine NMuLi cells (FIG. 5(b)). The cells were infected with ChAd155 RC1, ChAd155 RC2, ChAd83 RC1 or ChAd83 RC2 at a multiplicity of infection of 50, 250 or 1250 viral particles per cell (250 and 1250 for ChAd83 RC1). The cells were processed for western blot. The cells were harvested at two and seven days post infection, extracts prepared using standard methods and an equivalent amount of extract loaded onto SDS-PAGE gels. Following electrophoretic separation, the proteins were transferred onto nitrocellulose membranes, which were then probed with a commercially available monoclonal antibody to the rabies glycoprotein transgene.

[0192] FIG. 5(b) demonstrates that ChAd155 RC1, ChAd155 RC2, ChAd83 RC1 and ChAd83 RC2 express a transgene in murine NMuLi cells. Expression was observed at both two and five days post infection, indicated by the band of about 51 kDa, which corresponds to the expected molecular weight of the rabies glycoprotein (RG). ChAd155 demonstrated more efficient expression of the transgene than ChAd83. At two days post-infection, robust transgene expression by ChAd155 RC2 was observed even at the low multiplicity of 50 vp/cell, whereas robust transgene expression by ChAd155 RC1 was first observed at higher mois. Also, RC2 demonstrated more efficient transgene expression than RC1 in both ChAd155 and ChAd83 viral serotypes. RC2 was more robustly expressed than RC1 in each of the direct comparisons.

[0193] Western blot analysis was performed to compare the level of transgene expression by ChAd83 RC1, RC2 and RC3 in MRC5 cells (FIG. 5(c)). The cells were infected with ChAd83 RC1, RC2 or RC3 at a multiplicity of infection of 250 or 1250 viral particles per cell. The cells were harvested at two and seven days post infection, extracts prepared using standard methods and an equivalent amount of total cell extract loaded onto SDS-PAGE gels. Following electrophoretic separation, the proteins were transferred onto nitrocellulose membranes, which were then probed with a commercially available monoclonal antibody to the rabies glycoprotein transgene.

[0194] FIG. 5(c) demonstrates that all of ChAd83 RC1, RC2 and RC3 express a transgene in MRC5 cells. Expression was observed at both two and seven days post infection, indicated by the band of about 51 kDa, which corresponds to the expected molecular weight of the rabies glycoprotein (RG). The ChAd83 RC2 vector produced a higher level of transgene expression than the ChAd83 RC1 and RC3 vectors at both two and seven days post-infection. There was no rabies glycoprotein detection for the RC1 and RC3 vectors at 7 days.

Example 5: Construction of Alternative Chimpanzee Adenoviruses with a Single Expression Cassette

[0195] As in Example 1, wild type chimpanzee adenoviruses type 155 (ChAd155) (WO 2016/198621) isolated from healthy chimpanzees using standard procedures were constructed as replication defective viruses as described in Sci Transl Med (2012) 4:1 and WO 2010/086189.

[0196] In Example 5, the ChAd155 is constructed by inserting a single transgene expression cassette. This expression cassette comprises the classical human CMV (hCMV) promoter, F0.DELTA.TM, N and M2-1 RSV antigens and, optionally, a WPRE enhancer. This vector is shown in FIG. 6. The expression cassette is inserted into the E1 region of the adeno virus (after the E1 region has been deleted).

[0197] The ChAd155 shown in FIG. 6 comprises a transgene encoding all of the RSV F0.DELTA.TM, M2-1 and N antigens, wherein a self-cleavage site ("2A") is included between the RSV F0.DELTA.TM antigen and the composite RSV N.M2-1 antigen, in which a flexible linker is included between the RSV M2-1 and N antigens.

[0198] The ChAd155 RSV vector of Example 5 comprises the polynucleotide of SEQ ID NO: 4 and encodes the polypeptide of SEQ ID NO: 5.

Example 6: Construction of a Chimpanzee Adenoviruses with a Dual Expression Cassette

[0199] Again, wild type chimpanzee adenoviruses type 155 (ChAd155) (WO 2016/198621) isolated from healthy chimpanzees using standard procedures were constructed as replication defective viruses as described in Sci Transl Med (2012) 4:1 and WO 2010/086189.

[0200] The ChAd155 of Example 6 is constructed by inserting two transgene expression cassettes into two different locations in the adenovirus:

[0201] (1) The first expression cassette components comprise the classical human CMV (hCMV) promoter and N.M2-1 RSV composite antigen. This first expression cassette is inserted into the E1 region of the adenovirus (after the E1 region has been deleted).

[0202] (2) The second expression cassette comprises an enhanced classical human CMV (enhanced hCMV) promoter, the F0.DELTA.TM RSV antigen and a WPRE enhancer. This first expression cassette is inserted into the HE2 region of the adenovirus (after the HE2 region has been deleted).

[0203] This vector comprising a dual expression cassette is shown in FIG. 7.

[0204] In the construct of FIG. 7, Ad5E4orf6 has been substituted into the early gene 4 (E4) region. The substitution is necessary to increase the productivity in HEK 293 cells.

Example 7: Transgene Expression from the Dual Expression Cassette of Example 6

[0205] Western blot analysis was performed to compare the level of transgene expression in the ChAd155 vector of Example 6 (labelled "Dual" or "Dual cassette" in the figures) in MRC5 cells with:

[0206] (i) a vector comprising a single F expression cassette (ChAd155-F0.DELTA.TM, labelled "F0.DELTA.Tm"),

[0207] (ii) a vector comprising a single NM2 expression cassette (ChAd155-NM2, labelled "NM2-1"), and

[0208] (iii) the vector of Example 5 comprising a single expression cassette containing the F and N.M2-1 RSV antigens (ChAd155-F0.DELTA.TM.NM2, also labelled "RSV")

[0209] The western blot analysis is shown in FIG. 8 and FIG. 9.

[0210] As shown in FIG. 8, the cells were infected with ChAd155-F0.DELTA.TM, ChAd155-F0.DELTA.TM.NM2 ("RSV") or the ChAd155 dual cassette of Example 6 at a multiplicity of infection of 500 viral particles per cell. In addition, cells were infected with ChAd155-F0.DELTA.TM.NM2 ("RSV") at a multiplicity of infection of 500 or 1250 viral particles per cell. The cells were harvested at 48 hours and 96 hours post infection, extracts prepared using standard methods and an equivalent amount of total cell extract loaded onto SDS-PAGE gels.

[0211] FIG. 8 shows that the ChAd155 dual cassette provides an expression level of the F antigen which is comparable to ChAd155F0ATM and higher than ChAd155-F.DELTA.TM.NM2 in MRC5 cells.

[0212] As shown in FIG. 9, the cells were infected with ChAd155-NM2, ChAd155-F0.DELTA.TM.NM2 ("RSV") or the ChAd155 dual cassette of Example 6 at a multiplicity of infection of 250 and 1250 viral particles per cell. The cells were harvested at 48 hours post infection, extracts prepared using standard methods and an equivalent amount of total cell extract loaded onto SDS-PAGE gels.

[0213] In FIG. 9, the ChAd155 dual cassette provides NM2-1 expression level at least comparable to the ChAd155-NM2 single vector and higher than ChAd155-F.DELTA.TM.NM2 ("RSV") in MRC5 cells.

Example 8: Immunogencity of the Dual Expression Cassette of Example 6

[0214] The immunogenicity of the dual expression cassette of Example 6 was evaluated in CD1 outbred mice (10 per group). The experiment was performed by injecting 5.times.10.sup.8 viral particles intramuscularly into the mice. The B-cell response was measured at 4 and 8 weeks after the immunization by measuring the RSV neutralising titres. Each dot represents the response in a single mouse, and the line corresponds to the mean for each dose group. The results of this analysis are shown in FIG. 10.

[0215] FIG. 10 shows that the ChAd155 dual cassette provides a B-cell response comparable to ChAd155F0.DELTA.TM and higher than that produced by ChAd155-F0.DELTA.TM.NM2 ("RSV").

[0216] The immunogenicity of the dual expression cassette of Example 6 was also evaluated in BALB/c inbred mice (48, 11 or 8 per group). The experiment was performed by injecting 10.sup.7 or 10.sup.6 viral particles intramuscularly. The T-cell response was measured 3 weeks after the immunization by ex vivo IFN-gamma enzyme-linked immunospot (ELISpot) using a M2 peptide T cell epitope mapped in BALB/c mice. The results are shown in FIG. 11, expressed as IFN-gamma Spot Forming Cells (SFC) per million splenocytes. Each dot represents the response in a single mouse, and the line corresponds to the mean for each dose group. Injected dose in number of virus particles are shown on the x axis. The results are shown in FIG. 11. FIG. 11 shows that the ChAd155 dual cassette provides a T-cell response higher than that produced by the single cassette ChAd155-F0.DELTA.TM.NM2 ("triple RSV", the results for which are obtained from historical data). This difference in response is greater for the 10.sup.6 vp dose.

[0217] FIG. 11 refers to "# positive mice", i.e. the number of mice which responded to the vaccine.

Example 9: Immunogencity of the Dual Expression Cassette of Example 6 in Cows

[0218] The study design is detailed in Table 2 below:

TABLE-US-00002 No. End of Group Cows Vaccine Route Dose Immunization Study Gp1 4 ChAd155 Intramuscular 1 .times. 10.sup.11 D0 D90 single RSV (IM) Gp2 4 ChAd155 Intramuscular 1 .times. 10.sup.11 D0 D90 dual RSV (IM) Gp3 4 Saline Intramuscular N/A D0 D90 (IM)

[0219] The "ChAd155 single RSV" is the ChAd155 of Example 5, and the "ChAd155 dual RSV" is the ChAd155 of Example 6.

[0220] A total of 12 adult cows were enrolled in the study. The cows ranged in age from 2.7 years to 7.8 years and had a mean range of 4.8 years.

[0221] Before they were enrolled in the study, the cows were pre-screened for bovine RSV (BRSV) antibodies by ELISA. This allowed study groups to be established that had a similar distribution and mean BRSV Ab titer (so as to not bias any of the groups).

[0222] Samples were collected from the cows before vaccination (D-5 or D0) and after vaccination (D7,10,14,28,60,90). In this study, the cows were vaccinated with 1.times.10{circumflex over ( )}11 viral particles of one of the two vaccines or with saline on day zero (D0).

[0223] A Bronchoalveolar lavage (BAL) was performed at day -5, 7, 10 or 28 after vaccination to isolate T cells in the lungs of the cow. Then IFN-gamma cytokine production of the CD4+ and CD8+ T cells upon stimulation with RSV antigens (in the form of peptide pools) encoded in the vaccines was detected using intracellular cytokine staining (ICS) (i.e. IFN.gamma. ICS was used to detect the lung T cell responses in the animals). The results of this experiment are shown in FIGS. 12A and 12B. It can be concluded from this experiment that the ChAd155-dual RSV induces consistent RSV-specific CD4+ and CD8+ responses in Bronchoalveolar lavage (BAL).

[0224] Blood samples were also taken from the cows on day 0, 14, 28, 60 and 90 after vaccination in order for IFN-gamma cytokine production of the RSV-specific CD4+ and CD8+ responses of the peripheral blood mononuclear cells (PBMC) to be detected using intracellular cytokine staining (ICS) (i.e. IFN.gamma. ICS was used to detect the peripheral T cell responses). The results of this experiment are shown in FIGS. 13A and 13B. Based on these results, it can be concluded that the ChAd155-dual RSV consistently expand the pre-existing RSV-specific CD4+ and CD8+ responses in PBMC.

[0225] The blood samples were also used to detect neutralising antibodies (nAbs) for RSV in the serum (i.e. the peripheral humoral response was detected). The results of this experiment are shown in FIGS. 14A and 14B. These results show that the ChAd155-dual RSV boosts RSV nAbs in serum which are maintained at levels higher than baseline 3 months after vaccination.

Example 10: Immunogenicity of ChAd155 Dual Encoding Rabies G and RSV NM2 Proteins

[0226] Three different ChAd155 vectors used constructed in this experiment:

[0227] ChAd155 encoding both rabies G (RG) and RSV NM2 proteins (called "ChAd155 dual" in this example, and ChAd155 dual hCMV NM 2-1--CASI RG WPRE);

[0228] ChAd155 encoding just the rabies G (RG) protein (called "ChAd155 RG" in this example, and ChAd155(.DELTA.E4)CASI RG WPRE); and

[0229] The ChAd155 vector shown in FIG. 6, i.e. the vector with transgene encoding all of the RSV F0.DELTA.TM, M2-1 and N antigens (called "ChAd155 RSV").

[0230] Three different doses of the ChAd155 dual adenovirus were administered to mice: a highest dose of 10.sup.7 viral particles, and a middle dose of 10.sup.6 viral particles, and a lowest dose of 10.sup.5 viral particles.

[0231] Two different doses of the ChAd155 RG and ChAd155 RSV vectors were administered to mice. For the ChAd155 RSV, this was a higher dose of 10.sup.7 vaccine particles, and a lower dose of 10.sup.6 vaccine particles. For the ChAd155 RG, this was a higher dose of 10.sup.6 vaccine particles, and a lower dose of 10.sup.5 vaccine particles. Mice were sacrificed 3 weeks later and splenocytes tested by IFN.gamma. ELISpot for T cell response to the vaccine encoded antigens.

[0232] The results of this experiment are shown in FIGS. 15A, 15B and 15C. As can be seen from FIGS. 15A, 15B and 15C, the ChAd155 dual RG-NM2 vector shows overall comparable immune responses to the vectors encoding each of the RG and NM2 antigens alone.

[0233] FIG. 15C compares the cumulative response to all encoded antigens at the common 10.sup.6 vp dosage used for all three different vectors. The rabies G protein is listed twice (G1 and G2) as two pools of overlapping peptides were used to cover the whole sequence of the protein

[0234] Therefore, placing the two antigens in the same vector still produces a comparable immune response while allowing antigens for different pathogens to be provided in the same vector.

Example 11: Expression of ChAd155 Dual Encoding Rabies G and RSV NM2 Proteins in HeLa Cells

[0235] In the experiments of Example 11, HeLa cells were infected with the purified "ChAd155 dual", "ChAd155 RG" and "ChAd155 RSV" used in Example 10.

[0236] Multiplicities of infection (MOI) of 50, 250 and 1250 were used in this experiment.

[0237] In order to obtain the Western Blot shown in FIG. 16A (obtained under reducing conditions), the cell lysate was harvested 48 hours post-infection. The estimated size of the NM2-1 is 65 kDa. FIG. 16A shows a comparable expression level for ChAd155 dual cassette and ChAd155 NM2-1. In addition, the NM2-1 expression level was higher for the ChAd155 dual cassette than the ChAd155 RSV vector.

[0238] To obtain the Western Blot shown in FIG. 16B, the supernatent was harvested 48 hours post-infection. The estimated size of the rabies glycoprotein is 57.6 kDa. FIG. 16B shows a comparable expression level for the ChAd155 dual and ChAd155 RG adenoviruses.

[0239] In addition, infectivity data was also collected using the four different vectors. The infectivity of purified virus was evaluated in adherent Procell 92 cells by Hexon Immunostaining. The results are given in Table 3 below (vp=virus particle, ifu=infectious unit, and R is the ratio between these two numbers). The infectivity results indicate that all of the vectors have similar infectivity. In addition, as all of the R values were below 300, the infectivity of all vectors was deemed to be within the range of acceptability.

TABLE-US-00003 TABLE 3 Vp/ml Ifu/ml R (vp/ifu) ChAd155 hCMV NM 2-1 - 5.51E+11 4.53E+09 122 CASI RG WPRE ChAd155(.DELTA.E4)hCMV-RSV 1.12E+11 1.05E+09 107 ChAd155(.DELTA.E4)hCMV NM2-1 5.68E+11 4.26E+09 133 ChAd155(.DELTA.E4)CASI RG WPRE 3.48E+11 3.35E+09 104

Example 12: Immunogenicity of ChAd155 Dual Encoding CRPV E2 and E1 Proteins

[0240] Two different ChAd155 vectors were constructed in this experiment:

[0241] ChAd155 encoding a modified CRPV E2 protein in a first expression cassette, and a modified CRPV E1 protein in a second expression cassette (called "CRPV Dual"); and

[0242] ChAd155 encoding a fusion of the modified CRPV E2 and E1 proteins in a single expression cassette (called "CRPV Fusion")

[0243] Two different doses of the two adeno vectors were administered to mice: a higher dose of 10.sup.7 viral particles, and a lower dose of 10.sup.6 viral particles. The results of this experiment are shown in FIG. 17. FIG. 17 is a IFN.gamma. ELISpot on splenocytes 3 weeks post vaccination. A statistical analysis was performed on the results and the differences between the response from the different vectors was not deemed to be statistically significant. However, as can be seen from FIG. 17, the ChAd155 CRPV Dual vectors show increased frequency of responding mice at lowest dosage than the CRPV Fusion vectors (6/6 positive responding mice for the 10.sup.6 dose of the CRPV dual vector, but only 4/6 positive responding mice for the 10.sup.6 dose of the CRPV fusion vector.

Example 13: Expression of ChAd155 Dual Encoding CRPV E2 and E1 Proteins

[0244] The two different ChAd155 vectors used in Example 12 were also used in Example 13.

[0245] Multiplicities of infection (MOI) of 250 and 1250 were used in this experiment. The cell lysate was harvested 48 hours post-infection. The estimated size of the modified E1 protein is 48 kDa, the modified E2 protein is 35 kDa, and the fusion protein containing both the modified E1 and E2 proteins is 88 kDa.

[0246] FIG. 18 shows a Western blot (obtained under reducing conditions) illustrating that there was better expression of the modified E1 and E2 proteins by the CRPV dual vector than the CRPV fusion vector. The "Pvj" columns shown in FIG. 18 are the controls used.

[0247] In addition, infectivity data was collected using the two different vectors. The infectivity of purified virus was evaluated in adherent Procell 92 cells by Hexon Immunostaining. The results are given in Table 4 below (vp=virus particle, ifu=infectious unit, and R is the ratio between these two numbers). The infectivity results indicate that the two vectors have similar infectivity. In addition, as all of the R values were below 300, the infectivity of all vectors was deemed to be within the range of acceptability.

TABLE-US-00004 TABLE 4 Vp/ml Ifu/ml R (vp/ifu) ChAd155 hCMV CRPV 4.52E+11 2.97E+09 153 DE2DE1 Fusion HA WPRE "CRPV Fusion" ChAd155 hCMV CRPV DE2 6.20E+11 4.39E+09 143 HA WPRE - Enh CMV CRPV DE1 HA WPRE "CRPV Dual"

TABLE-US-00005 DESCRIPTION OF THE SEQUENCES Polynucleotide sequence encoding wild type ChAd155 SEQ ID NO: 1 CATCATCAATAATATACCTTATTTTGGATTGAAGCCAATATGATAATGAGATGGGCGGCGCGGGGCGGGAG GCGGGTCCGGGGGCGGGCCGGCGGGCGGGGCGGTGTGGCGGAAGTGGACTTTGTAAGTGTGGCGGATGTGACTT- GCT AGTGCCGGGCGCGGTAAAAGTGACGTTTTCCGTGCGCGACAACGCCCACGGGAAGTGACATTTTTCCCGCGGTT- TTT ACCGGATGTTGTAGTGAATTTGGGCGTAACCAAGTAAGATTTGGCCATTTTCGCGGGAAAACTGAAACGGGGAA- GTG AAATCTGATTAATTTCGCGTTAGTCATACCGCGTAATATTTGTCGAGGGCCGAGGGACTTTGGCCGATTACGTG- GAG GACTCGCCCAGGTGTTTTTTGAGGTGAATTTCCGCGTTCCGGGTCAAAGTCTCCGTTTTATTATTATAGTCAGC- TGA CGCGGAGTGTATTTATACCCTCTGATCTCGTCAAGTGGCCACTCTTGAGTGCCAGCGAGTAGAGTTTTCTCCTC- TGC CGCTCTCCGCTCCGCTCCGCTCGGCTCTGACACCGGGGAAAAAATGAGACATTTCACCTACGATGGCGGTGTGC- TCA CCGGCCAGCTGGCTGCTGAAGTCCTGGACACCCTGATCGAGGAGGTATTGGCCGATAATTATCCTCCCTCGACT- CCT TTTGAGCCACCTACACTTCACGAACTCTACGATCTGGATGTGGTGGGGCCCAGCGATCCGAACGAGCAGGCGGT- TTC CAGTTTTTTTCCAGAGTCCATGTTGTTGGCCAGCCAGGAGGGGGTCGAACTTGAGACCCCTCCTCCGATCGTGG- ATT CCCCCGATCCGCCGCAGCTGACTAGGCAGCCCGAGCGCTGTGCGGGACCTGAGACTATGCCCCAGCTGCTACCT- GAG GTGATCGATCTCACCTGTAATGAGTCTGGTTTTCCACCCAGCGAGGATGAGGACGAAGAGGGTGAGCAGTTTGT- GTT AGATTCTGTGGAACAACCCGGGCGAGGATGCAGGTCTTGTCAATATCACCGGAAAAACACAGGAGACTCCCAGA- TTA TGTGTTCTCTGTGTTATATGAAGATGACCTGTATGTTTATTTACAGTAAGTTTATCATCTGTGGGCAGGTGGGC- TAT AGTGTGGGTGGTGGTCTTTGGGGGGTTTTTTAATATATGTCAGGGGTTATGCTGAAGACTTTTTTATTGTGATT- TTT AAAGGTCCAGTGTCTGAGCCCGAGCAAGAACCTGAACCGGAGCCTGAGCCTTCTCGCCCCAGGAGAAAGCCTGT- AAT CTTAACTAGACCCAGCGCACCGGTAGCGAGAGGCCTCAGCAGCGCGGAGACCACCGACTCCGGTGCTTCCTCAT- CAC CCCCGGAGATTCACCCCCTGGTGCCCCTGTGTCCCGTTAAGCCCGTTGCCGTGAGAGTCAGTGGGCGGCGGTCT- GCT GTGGAGTGCATTGAGGACTTGCTTTTTGATTCACAGGAACCTTTGGACTTGAGCTTGAAACGCCCCAGGCATTA- AAC CTGGTCACCTGGACTGAATGAGTTGACGCCTATGTTTGCTTTTGAATGACTTAATGTGTATAGATAATAAAGAG- TGA GATAATGTTTTAATTGCATGGTGTGTTTAACTTGGGCGGAGTCTGCTGGGTATATAAGCTTCCCTGGGCTAAAC- TTG GTTACACTTGACCTCATGGAGGCCTGGGAGTGTTTGGAGAACTTTGCCGGAGTTCGTGCCTTGCTGGACGAGAG- CTC TAACAATACCTCTTGGTGGTGGAGGTATTTGTGGGGCTCTCCCCAGGGCAAGTTAGTTTGTAGAATCAAGGAGG- ATT ACAAGTGGGAATTTGAAGAGCTTTTGAAATCCTGTGGTGAGCTATTGGATTCTTTGAATCTAGGCCACCAGGCT- CTC TTCCAGGAGAAGGTCATCAGGACTTTGGATTTTTCCACACCGGGGCGCATTGCAGCCGCGGTTGCTTTTCTAGC- TTT TTTGAAGGATAGATGGAGCGAAGAGACCCACTTGAGTTCGGGCTACGTCCTGGATTTTCTGGCCATGCAACTGT- GGA GAGCATGGATCAGACACAAGAACAGGCTGCAACTGTTGTCTTCCGTCCGCCCGTTGCTGATTCCGGCGGAGGAG- CAA CAGGCCGGGTCAGAGGACCGGGCCCGTCGGGATCCGGAGGAGAGGGCACCGAGGCCGGGCGAGAGGAGCGCGCT- GAA CCTGGGAACCGGGCTGAGCGGCCATCCACATCGGGAGTGAATGTCGGGCAGGTGGTGGATCTTTTTCCAGAACT- GCG GCGGATTTTGACTATTAGGGAGGATGGGCAATTTGTTAAGGGTCTTAAGAGGGAGAGGGGGGCTTCTGAGCATA- ACG AGGAGGCCAGTAATTTAGCTTTTAGCTTGATGACCAGACACCGTCCAGAGTGCATCACTTTTCAGCAGATTAAG- GAC AATTGTGCCAATGAGTTGGATCTGTTGGGTCAGAAGTATAGCATAGAGCAGCTGACCACTTACTGGCTGCAGCC- GGG TGATGATCTGGAGGAAGCTATTAGGGTGTATGCTAAGGTGGCCCTGCGGCCCGATTGCAAGTACAAGCTCAAGG- GGC TGGTGAATATCAGGAATTGTTGCTACATTTCTGGCAACGGGGCGGAGGTGGAGATAGAGACCGAAGACAGGGTG- GCT TTCAGATGCAGCATGATGAATATGTGGCCGGGGGTGCTGGGCATGGACGGGGTGGTGATTATGAATGTGAGGTT- CAC GGGGCCCAACTTTAACGGCACGGTGTTTTTGGGGAACACCAACCTGGTCCTGCACGGGGTGAGCTTCTATGGGT- TTA ACAACACCTGTGTGGAGGCCTGGACCGATGTGAAGGTCCGCGGTTGCGCCTTTTATGGATGTTGGAAGGCCATA- GTG AGCCGCCCTAAGAGCAGGAGTTCCATTAAGAAATGCTTGTTTGAGAGGTGCACCTTGGGGATCCTGGCCGAGGG- CAA CTGCAGGGTGCGCCACAATGTGGCCTCCGAGTGCGGTTGCTTCATGCTAGTCAAGAGCGTGGCGGTAATCAAGC- ATA ATATGGTGTGCGGCAACAGCGAGGACAAGGCCTCACAGATGCTGACCTGCACGGATGGCAACTGCCACTTGCTG- AAG ACCATCCATGTAACCAGCCACAGCCGGAAGGCCTGGCCCGTGTTCGAGCACAACTTGCTGACCCGCTGCTCCTT- GCA TCTGGGCAACAGGCGGGGGGTGTTCCTGCCCTATCAATGCAACTTTAGTCACACCAAGATCTTGCTAGAGCCCG- AGA GCATGTCCAAGGTGAACTTGAACGGGGTGTTTGACATGACCATGAAGATCTGGAAGGTGCTGAGGTACGACGAG- ACC AGGTCCCGGTGCAGACCCTGCGAGTGCGGGGGCAAGCATATGAGGAACCAGCCCGTGATGCTGGATGTGACCGA- GGA GCTGAGGACAGACCACTTGGTTCTGGCCTGCACCAGGGCCGAGTTTGGTTCTAGCGATGAAGACACAGATTGAG- GTG GGTGAGTGGGCGTGGCCTGGGGTGGTCATGAAAATATATAAGTTGGGGGTCTTAGGGTCTCTTTATTTGTGTTG- CAG AGACCGCCGGAGCCATGAGCGGGAGCAGCAGCAGCAGCAGTAGCAGCAGCGCCTTGGATGGCAGCATCGTGAGC- CCT TATTTGACGACGCGGATGCCCCACTGGGCCGGGGTGCGTCAGAATGTGATGGGCTCCAGCATCGACGGCCGACC- CGT CCTGCCCGCAAATTCCGCCACGCTGACCTATGCGACCGTCGCGGGGACGCCGTTGGACGCCACCGCCGCCGCCG- CCG CCACCGCAGCCGCCTCGGCCGTGCGCAGCCTGGCCACGGACTTTGCATTCCTGGGACCACTGGCGACAGGGGCT- ACT TCTCGGGCCGCTGCTGCCGCCGTTCGCGATGACAAGCTGACCGCCCTGCTGGCGCAGTTGGATGCGCTTACTCG- GGA ACTGGGTGACCTTTCTCAGCAGGTCATGGCCCTGCGCCAGCAGGTCTCCTCCCTGCAAGCTGGCGGGAATGCTT- CTC CCACAAATGCCGTTTAAGATAAATAAAACCAGACTCTGTTTGGATTAAAGAAAAGTAGCAAGTGCATTGCTCTC- TTT ATTTCATAATTTTCCGCGCGCGATAGGCCCTAGACCAGCGTTCTCGGTCGTTGAGGGTGCGGTGTATCTTCTCC- AGG ACGTGGTAGAGGTGGCTCTGGACGTTGAGATACATGGGCATGAGCCCGTCCCGGGGGTGGAGGTAGCACCACTG- CAG AGCTTCATGCTCCGGGGTGGTGTTGTAGATGATCCAGTCGTAGCAGGAGCGCTGGGCATGGTGCCTAAAAATGT- CCT TCAGCAGCAGGCCGATGGCCAGGGGGAGGCCCTTGGTGTAAGTGTTTACAAAACGGTTAAGTTGGGAAGGGTGC- ATT CGGGGAGAGATGATGTGCATCTTGGACTGTATTTTTAGATTGGCGATGTTTCCGCCCAGATCCCTTCTGGGATT- CAT GTTGTGCAGGACCACCAGTACAGTGTATCCGGTGCACTTGGGGAATTTGTCATGCAGCTTAGAGGGAAAAGCGT- GGA AGAACTTGGAGACGCCTTTGTGGCCTCCCAGATTTTCCATGCATTCGTCCATGATGATGGCAATGGGCCCGCGG- GAG GCAGCTTGGGCAAAGATATTTCTGGGGTCGCTGACGTCGTAGTTGTGTTCCAGGGTGAGGTCGTCATAGGCCAT- TTT TACAAAGCGCGGGCGGAGGGTGCCCGACTGGGGGATGATGGTCCCCTCTGGCCCTGGGGCGTAGTTGCCCTCGC- AGA TCTGCATTTCCCAGGCCTTAATCTCGGAGGGGGGAATCATATCCACCTGCGGGGCGATGAAGAAAACGGTTTCC- GGA GCCGGGGAGATTAACTGGGATGAGAGCAGGTTTCTAAGCAGCTGTGATTTTCCACAACCGGTGGGCCCATAAAT- AAC ACCTATAACCGGTTGCAGCTGGTAGTTTAGAGAGCTGCAGCTGCCGTCGTCCCGGAGGAGGGGGGCCACCTCGT- TGA GCATGTCCCTGACGCGCATGTTCTCCCCGACCAGATCCGCCAGAAGGCGCTCGCCGCCCAGGGACAGCAGCTCT- TGC AAGGAAGCAAAGTTTTTCAGCGGCTTGAGGCCGTCCGCCGTGGGCATGTTTTTCAGGGTCTGGCTCAGCAGCTC- CAG GCGGTCCCAGAGCTCGGTGACGTGCTCTACGGCATCTCTATCCAGCATATCTCCTCGTTTCGCGGGTTGGGGCG- ACT TTCGCTGTAGGGCACCAAGCGGTGGTCGTCCAGCGGGGCCAGAGTCATGTCCTTCCATGGGCGCAGGGTCCTCG- TCA GGGTGGTCTGGGTCACGGTGAAGGGGTGCGCTCCGGGCTGAGCGCTTGCCAAGGTGCGCTTGAGGCTGGTTCTG- CTG GTGCTGAAGCGCTGCCGGTCTTCGCCCTGCGCGTCGGCCAGGTAGCATTTGACCATGGTGTCATAGTCCAGCCC- CTC CGCGGCGTGTCCCTTGGCGCGCAGCTTGCCCTTGGAGGTGGCGCCGCACGAGGGGCAGAGCAGGCTCTTGAGCG- CGT AGAGCTTGGGGGCGAGGAAGACCGATTCGGGGGAGTAGGCGTCCGCGCCGCAGACCCCGCACACGGTCTCGCAC- TCC ACCAGCCAGGTGAGCTCGGGGCGCGCCGGGTCAAAAACCAGGTTTCCCCCATGCTTTTTGATGCGTTTCTTACC- TCG GGTCTCCATGAGGTGGTGTCCCCGCTCGGTGACGAAGAGGCTGTCCGTGTCTCCGTAGACCGACTTGAGGGGTC- TTT TCTCCAGGGGGGTCCCTCGGTCTTCCTCGTAGAGGAACTCGGACCACTCTGAGACGAAGGCCCGCGTCCAGGCC- AGG ACGAAGGAGGCTATGTGGGAGGGGTAGCGGTCGTTGTCCACTAGGGGGTCCACCTTCTCCAAGGTGTGAAGACA- CAT GTCGCCTTCCTCGGCGTCCAGGAAGGTGATTGGCTTGTAGGTGTAGGCCACGTGACCGGGGGTTCCTGACGGGG- GGG TATAAAAGGGGGTGGGGGCGCGCTCGTCGTCACTCTCTTCCGCATCGCTGTCTGCGAGGGCCAGCTGCTGGGGT- GAG TATTCCCTCTCGAAGGCGGGCATGACCTCCGCGCTGAGGTTGTCAGTTTCCAAAAACGAGGAGGATTTGATGTT- CAC CTGTCCCGAGGTGATACCTTTGAGGGTACCCGCGTCCATCTGGTCAGAAAACACGATCTTTTTATTGTCCAGCT- TGG TGGCGAACGACCCGTAGAGGGCGTTGGAGAGCAGCTTGGCGATGGAGCGCAGGGTCTGGTTCTTGTCCCTGTCG- GCG

CGCTCCTTGGCCGCGATGTTGAGCTGCACGTACTCGCGCGCGACGCAGCGCCACTCGGGGAAGACGGTGGTGCG- CTC GTCGGGCACCAGGCGCACGCGCCAGCCGCGGTTGTGCAGGGTGACCAGGTCCACGCTGGTGGCGACCTCGCCGC- GCA GGCGCTCGTTGGTCCAGCAGAGACGGCCGCCCTTGCGCGAGCAGAAGGGGGGCAGGGGGTCGAGCTGGGTCTCG- TCC GGGGGGTCCGCGTCCACGGTGAAAACCCCGGGGCGCAGGCGCGCGTCGAAGTAGTCTATCTTGCAACCTTGCAT- GTC CAGCGCCTGCTGCCAGTCGCGGGCGGCGAGCGCGCGCTCGTAGGGGTTGAGCGGCGGGCCCCAGGGCATGGGGT- GGG TGAGTGCGGAGGCGTACATGCCGCAGATGTCATAGACGTAGAGGGGCTCCCGCAGGACCCCGATGTAGGTGGGG- TAG CAGCGGCCGCCGCGGATGCTGGCGCGCACGTAGTCATACAGCTCGTGCGAGGGGGCGAGGAGGTCGGGGCCCAG- GTT GGTGCGGGCGGGGCGCTCCGCGCGGAAGACGATCTGCCTGAAGATGGCATGCGAGTTGGAAGAGATGGTGGGGC- GCT GGAAGACGTTGAAGCTGGCGTCCTGCAGGCCGACGGCGTCGCGCACGAAGGAGGCGTAGGAGTCGCGCAGCTTG- TGT ACCAGCTCGGCGGTGACCTGCACGTCGAGCGCGCAGTAGTCGAGGGTCTCGCGGATGATGTCATATTTAGCCTG- CCC CTTCTTTTTCCACAGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTACTCTTGGATCGGGAAACCGT- CCG GTTCCGAACGGTAAGAGCCTAGCATGTAGAACTGGTTGACGGCCTGGTAGGCGCAGCAGCCCTTCTCCACGGGG- AGG GCGTAGGCCTGCGCGGCCTTGCGGAGCGAGGTGTGGGTCAGGGCGAAGGTGTCCCTGACCATGACTTTGAGGTA- CTG GTGCTTGAAGTCGGAGTCGTCGCAGCCGCCCCGCTCCCAGAGCGAGAAGTCGGTGCGCTTCTTGGAGCGGGGGT- TGG GCAGAGCGAAGGTGACATCGTTGAAGAGGATTTTGCCCGCGCGGGGCATGAAGTTGCGGGTGATGCGGAAGGGC- CCC GGCACTTCAGAGCGGTTGTTGATGACCTGGGCGGCGAGCACGATCTCGTCGAAGCCGTTGATGTTGTGGCCCAC- GAT GTAGAGTTCCAGGAAGCGGGGCCGGCCCTTTACGGTGGGCAGCTTCTTTAGCTCTTCGTAGGTGAGCTCCTCGG- GCG AGGCGAGGCCGTGCTCGGCCAGGGCCCAGTCCGCGAGGTGCGGGTTGTCTCTGAGGAAGGACTTCCAGAGGTCG- CGG GCCAGGAGGGTCTGCAGGCGGTCTCTGAAGGTCCTGAACTGGCGGCCCACGGCCATTTTTTCGGGGGTGATGCA- GTA GAAGGTGAGGGGGTCTTGCTGCCAGCGGTCCCAGTCGAGCTGCAGGGCGAGGTCGCGCGCGGCGGTGACCAGGC- GCT CGTCGCCCCCGAATTTCATGACCAGCATGAAGGGCACGAGCTGCTTTCCGAAGGCCCCCATCCAAGTGTAGGTC- TCT ACATCGTAGGTGACAAAGAGGCGCTCCGTGCGAGGATGCGAGCCGATCGGGAAGAACTGGATCTCCCGCCACCA- GTT GGAGGAGTGGCTGTTGATGTGGTGGAAGTAGAAGTCCCGTCGCCGGGCCGAACACTCGTGCTGGCTTTTGTAAA- AGC GAGCGCAGTACTGGCAGCGCTGCACGGGCTGTACCTCATGCACGAGATGCACCTTTCGCCCGCGCACGAGGAAG- CCG AGGGGAAATCTGAGCCCCCCGCCTGGCTCGCGGCATGGCTGGTTCTCTTCTACTTTGGATGCGTGTCCGTCTCC- GTC TGGCTCCTCGAGGGGTGTTACGGTGGAGCGGACCACCACGCCGCGCGAGCCGCAGGTCCAGATATCGGCGCGCG- GCG GTCGGAGTTTGATGACGACATCGCGCAGCTGGGAGCTGTCCATGGTCTGGAGCTCCCGCGGCGGCGGCAGGTCA- GCC GGGAGTTCTTGCAGGTTCACCTCGCAGAGTCGGGCCAGGGCGCGGGGCAGGTCTAGGTGGTACCTGATCTCTAG- GGG CGTGTTGGTGGCGGCGTCGATGGCTTGCAGGAGCCCGCAGCCCCGGGGGGCGACGACGGTGCCCCGCGGGGTGG- TGG TGGTGGTGGCGGTGCAGCTCAGAAGCGGTGCCGCGGGCGGGCCCCCGGAGGTAGGGGGGGCTCCGGTCCCGCGG- GCA GGGGCGGCAGCGGCACGTCGGCGTGGAGCGCGGGCAGGAGTTGGTGCTGTGCCCGGAGGTTGCTGGCGAAGGCG- ACG ACGCGGCGGTTGATCTCCTGGATCTGGCGCCTCTGCGTGAAGACGACGGGCCCGGTGAGCTTGAACCTGAAAGA- GAG TTCGACAGAATCAATCTCGGTGTCATTGACCGCGGCCTGGCGCAGGATCTCCTGCACGTCTCCCGAGTTGTCTT- GGT AGGCGATCTCGGCCATGAACTGCTCGATCTCTTCCTCCTGGAGGTCTCCGCGTCCGGCGCGTTCCACGGTGGCC- GCC AGGTCGTTGGAGATGCGCCCCATGAGCTGCGAGAAGGCGTTGAGTCCGCCCTCGTTCCAGACTCGGCTGTAGAC- CAC GCCCCCCTGGTCATCGCGGGCGCGCATGACCACCTGCGCGAGGTTGAGCTCCACGTGCCGCGCGAAGACGGCGT- AGT TGCGCAGACGCTGGAAGAGGTAGTTGAGGGTGGTGGCGGTGTGCTCGGCCACGAAGAAGTTCATGACCCAGCGG- CGC AACGTGGATTCGTTGATGTCCCCCAAGGCCTCCAGCCGTTCCATGGCCTCGTAGAAGTCCACGGCGAAGTTGAA- AAA CTGGGAGTTGCGCGCCGACACGGTCAACTCCTCCTCCAGAAGACGGATGAGCTCGGCGACGGTGTCGCGCACCT- CGC GCTCGAAGGCTATGGGGATCTCTTCCTCCGCTAGCATCACCACCTCCTCCTCTTCCTCCTCTTCTGGCACTTCC- ATG ATGGCTTCCTCCTCTTCGGGGGGTGGCGGCGGCGGCGGTGGGGGAGGGGGCGCTCTGCGCCGGCGGCGGCGCAC- CGG GAGGCGGTCCACGAAGCGCGCGATCATCTCCCCGCGGCGGCGGCGCATGGTCTCGGTGACGGCGCGGCCGTTCT- CCC GGGGGCGCAGTTGGAAGACGCCGCCGGACATCTGGTGCTGGGGCGGGTGGCCGTGAGGCAGCGAGACGGCGCTG- ACG ATGCATCTCAACAATTGCTGCGTAGGTACGCCGCCGAGGGACCTGAGGGAGTCCATATCCACCGGATCCGAAAA- CCT TTCGAGGAAGGCGTCTAACCAGTCGCAGTCGCAAGGTAGGCTGAGCACCGTGGCGGGCGGCGGGGGGTGGGGGG- AGT GTCTGGCGGAGGTGCTGCTGATGATGTAATTGAAGTAGGCGGACTTGACACGGCGGATGGTCGACAGGAGCACC- ATG TCCTTGGGTCCGGCCTGCTGGATGCGGAGGCGGTCGGCTATGCCCCAGGCTTCGTTCTGGCATCGGCGCAGGTC- CTT GTAGTAGTCTTGCATGAGCCTTTCCACCGGCACCTCTTCTCCTTCCTCTTCTGCTTCTTCCATGTCTGCTTCGG- CCC TGGGGCGGCGCCGCGCCCCCCTGCCCCCCATGCGCGTGACCCCGAACCCCCTGAGCGGTTGGAGCAGGGCCAGG- TCG GCGACGACGCGCTCGGCCAGGATGGCCTGCTGCACCTGCGTGAGGGTGGTTTGGAAGTCATCCAAGTCCACGAA- GCG GTGGTAGGCGCCCGTGTTGATGGTGTAGGTGCAGTTGGCCATGACGGACCAGTTGACGGTCTGGTGGCCCGGTT- GCG ACATCTCGGTGTACCTGAGTCGCGAGTAGGCGCGGGAGTCGAAGACGTAGTCGTTGCAAGTCCGCACCAGGTAC- TGG TAGCCCACCAGGAAGTGCGGCGGCGGCTGGCGGTAGAGGGGCCAGCGCAGGGTGGCGGGGGCTCCGGGGGCCAG- GTC TTCCAGCATGAGGCGGTGGTAGGCGTAGATGTACCTGGACATCCAGGTGATACCCGCGGCGGTGGTGGAGGCGC- GCG GGAAGTCGCGCACCCGGTTCCAGATGTTGCGCAGGGGCAGAAAGTGCTCCATGGTAGGCGTGCTCTGTCCAGTC- AGA CGCGCGCAGTCGTTGATACTCTAGACCAGGGAAAACGAAAGCCGGTCAGCGGGCACTCTTCCGTGGTCTGGTGA- ATA GATCGCAAGGGTATCATGGCGGAGGGCCTCGGTTCGAGCCCCGGGTCCGGGCCGGACGGTCCGCCATGATCCAC- GCG GTTACCGCCCGCGTGTCGAACCCAGGTGTGCGACGTCAGACAACGGTGGAGTGTTCCTTTTGGCGTTTTTCTGG- CCG GGCGCCGGCGCCGCGTAAGAGACTAAGCCGCGAAAGCGAAAGCAGTAAGTGGCTCGCTCCCCGTAGCCGGAGGG- ATC CTTGCTAAGGGTTGCGTTGCGGCGAACCCCGGTTCGAATCCCGTACTCGGGCCGGCCGGACCCGCGGCTAAGGT- GTT GGATTGGCCTCCCCCTCGTATAAAGACCCCGCTTGCGGATTGACTCCGGACACGGGGACGAGCCCCTTTTATTT- TTG CTTTCCCCAGATGCATCCGGTGCTGCGGCAGATGCGCCCCCCGCCCCAGCAGCAGCAACAACACCAGCAAGAGC- GGC AGCAACAGCAGCGGGAGTCATGCAGGGCCCCCTCACCCACCCTCGGCGGGCCGGCCACCTCGGCGTCCGCGGCC- GTG TCTGGCGCCTGCGGCGGCGGCGGGGGGCCGGCTGACGACCCCGAGGAGCCCCCGCGGCGCAGGGCCAGACACTA- CCT GGACCTGGAGGAGGGCGAGGGCCTGGCGCGGCTGGGGGCGCCGTCTCCCGAGCGCCACCCGCGGGTGCAGCTGA- AGC GCGACTCGCGCGAGGCGTACGTGCCTCGGCAGAACCTGTTCAGGGACCGCGCGGGCGAGGAGCCCGAGGAGATG- CGG GACAGGAGGTTCAGCGCAGGGCGGGAGCTGCGGCAGGGGCTGAACCGCGAGCGGCTGCTGCGCGAGGAGGACTT- TGA GCCCGACGCGCGGACGGGGATCAGCCCCGCGCGCGCGCACGTGGCGGCCGCCGACCTGGTGACGGCGTACGAGC- AGA CGGTGAACCAGGAGATCAACTTCCAAAAGAGTTTCAACAACCACGTGCGCACGCTGGTGGCGCGCGAGGAGGTG- ACC ATCGGGCTGATGCACCTGTGGGACTTTGTAAGCGCGCTGGTGCAGAACCCCAACAGCAAGCCTCTGACGGCGCA- GCT GTTCCTGATAGTGCAGCACAGCAGGGACAACGAGGCGTTTAGGGACGCGCTGCTGAACATCACCGAGCCCGAGG- GTC GGTGGCTGCTGGACCTGATTAACATCCTGCAGAGCATAGTGGTGCAGGAGCGCAGCCTGAGCCTGGCCGACAAG- GTG GCGGCCATCAACTACTCGATGCTGAGCCTGGGCAAGTTTTACGCGCGCAAGATCTACCAGACGCCGTACGTGCC- CAT AGACAAGGAGGTGAAGATCGACGGTTTTTACATGCGCATGGCGCTGAAGGTGCTCACCCTGAGCGACGACCTGG- GCG TGTACCGCAACGAGCGCATCCACAAGGCCGTGAGCGTGAGCCGGCGGCGCGAGCTGAGCGACCGCGAGCTGATG- CAC AGCCTGCAGCGGGCGCTGGCGGGCGCCGGCAGCGGCGACAGGGAGGCGGAGTCCTACTTCGATGCGGGGGCGGA- CCT GCGCTGGGCGCCCAGCCGGCGGGCCCTGGAGGCCGCGGGGGTCCGCGAGGACTATGACGAGGACGGCGAGGAGG- ATG AGGAGTACGAGCTAGAGGAGGGCGAGTACCTGGACTAAACCGCGGGTGGTGTTTCCGGTAGATGCAAGACCCGA- ACG TGGTGGACCCGGCGCTGCGGGCGGCTCTGCAGAGCCAGCCGTCCGGCCTTAACTCCTCAGACGACTGGCGACAG- GTC ATGGACCGCATCATGTCGCTGACGGCGCGTAACCCGGACGCGTTCCGGCAGCAGCCGCAGGCCAACAGGCTCTC- CGC CATCCTGGAGGCGGTGGTGCCTGCGCGCTCGAACCCCACGCACGAGAAGGTGCTGGCCATAGTGAACGCGCTGG- CCG AGAACAGGGCCATCCGCCCGGACGAGGCCGGGCTGGTGTACGACGCGCTGCTGCAGCGCGTGGCCCGCTACAAC- AGC GGCAACGTGCAGACCAACCTGGACCGGCTGGTGGGGGACGTGCGCGAGGCGGTGGCGCAGCGCGAGCGCGCGGA- TCG GCAGGGCAACCTGGGCTCCATGGTGGCGCTGAATGCCTTCCTGAGCACGCAGCCGGCCAACGTGCCGCGGGGGC-

AGG AAGACTACACCAACTTTGTGAGCGCGCTGCGGCTGATGGTGACCGAGACCCCCCAGAGCGAGGTGTACCAGTCG- GGC CCGGACTACTTCTTCCAGACCAGCAGACAGGGCCTGCAGACGGTGAACCTGAGCCAGGCTTTCAAGAACCTGCG- GGG GCTGTGGGGCGTGAAGGCGCCCACCGGCGACCGGGCGACGGTGTCCAGCCTGCTGACGCCCAACTCGCGCCTGC- TGC TGCTGCTGATCGCGCCGTTCACGGACAGCGGCAGCGTGTCCCGGGACACCTACCTGGGGCACCTGCTGACCCTG- TAC CGCGAGGCCATCGGGCAGGCGCAGGTGGACGAGCACACCTTCCAGGAGATCACCAGCGTGAGCCGCGCGCTGGG- GCA GGAGGACACGAGCAGCCTGGAGGCGACTCTGAACTACCTGCTGACCAACCGGCGGCAGAAGATTCCCTCGCTGC- ACA GCCTGACCTCCGAGGAGGAGCGCATCTTGCGCTACGTGCAGCAGAGCGTGAGCCTGAACCTGATGCGCGACGGG- GTG ACGCCCAGCGTGGCGCTGGACATGACCGCGCGCAACATGGAACCGGGCATGTACGCCGCGCACCGGCCTTACAT- CAA CCGCCTGATGGACTACCTGCATCGCGCGGCGGCCGTGAACCCCGAGTACTTTACCAACGCCATCCTGAACCCGC- ACT GGCTCCCGCCGCCCGGGTTCTACAGCGGGGGCTTCGAGGTCCCGGAGACCAACGATGGCTTCCTGTGGGACGAC- ATG GACGACAGCGTGTTCTCCCCGCGGCCGCAGGCGCTGGCGGAAGCGTCCCTGCTGCGTCCCAAGAAGGAGGAGGA- GGA GGAGGCGAGTCGCCGCCGCGGCAGCAGCGGCGTGGCTTCTCTGTCCGAGCTGGGGGCGGCAGCCGCCGCGCGCC- CCG GGTCCCTGGGCGGCAGCCCCTTTCCGAGCCTGGTGGGGTCTCTGCACAGCGAGCGCACCACCCGCCCTCGGCTG- CTG GGCGAGGACGAGTACCTGAATAACTCCCTGCTGCAGCCGGTGCGGGAGAAAAACCTGCCTCCCGCCTTCCCCAA- CAA CGGGATAGAGAGCCTGGTGGACAAGATGAGCAGATGGAAGACCTATGCGCAGGAGCACAGGGACGCGCCTGCGC- TCC GGCCGCCCACGCGGCGCCAGCGCCACGACCGGCAGCGGGGGCTGGTGTGGGATGACGAGGACTCCGCGGACGAT- AGC AGCGTGCTGGACCTGGGAGGGAGCGGCAACCCGTTCGCGCACCTGCGCCCCCGCCTGGGGAGGATGTTTTAAAA- AAA AAAAAAAAAAGCAAGAAGCATGATGCAAAAATTAAATAAAACTCACCAAGGCCATGGCGACCGAGCGTTGGTTT- CTT GTGTTCCCTTCAGTATGCGGCGCGCGGCGATGTACCAGGAGGGACCTCCTCCCTCTTACGAGAGCGTGGTGGGC- GCG GCGGCGGCGGCGCCCTCTTCTCCCTTTGCGTCGCAGCTGCTGGAGCCGCCGTACGTGCCTCCGCGCTACCTGCG- GCC TACGGGGGGGAGAAACAGCATCCGTTACTCGGAGCTGGCGCCCCTGTTCGACACCACCCGGGTGTACCTGGTGG- ACA ACAAGTCGGCGGACGTGGCCTCCCTGAACTACCAGAACGACCACAGCAATTTTTTGACCACGGTCATCCAGAAC- AAT GACTACAGCCCGAGCGAGGCCAGCACCCAGACCATCAATCTGGATGACCGGTCGCACTGGGGCGGCGACCTGAA- AAC CATCCTGCACACCAACATGCCCAACGTGAACGAGTTCATGTTCACCAATAAGTTCAAGGCGCGGGTGATGGTGT- CGC GCTCGCACACCAAGGAAGACCGGGTGGAGCTGAAGTACGAGTGGGTGGAGTTCGAGCTGCCAGAGGGCAACTAC- TCC GAGACCATGACCATTGACCTGATGAACAACGCGATCGTGGAGCACTATCTGAAAGTGGGCAGGCAGAACGGGGT- CCT GGAGAGCGACATCGGGGTCAAGTTCGACACCAGGAACTTCCGCCTGGGGCTGGACCCCGTGACCGGGCTGGTTA- TGC CCGGGGTGTACACCAACGAGGCCTTCCATCCCGACATCATCCTGCTGCCCGGCTGCGGGGTGGACTTCACTTAC- AGC CGCCTGAGCAACCTCCTGGGCATCCGCAAGCGGCAGCCCTTCCAGGAGGGCTTCAGGATCACCTACGAGGACCT- GGA GGGGGGCAACATCCCCGCGCTCCTCGATGTGGAGGCCTACCAGGATAGCTTGAAGGAAAATGAGGCGGGACAGG- AGG ATACCGCCCCCGCCGCCTCCGCCGCCGCCGAGCAGGGCGAGGATGCTGCTGACACCGCGGCCGCGGACGGGGCA- GAG GCCGACCCCGCTATGGTGGTGGAGGCTCCCGAGCAGGAGGAGGACATGAATGACAGTGCGGTGCGCGGAGACAC- CTT CGTCACCCGGGGGGAGGAAAAGCAAGCGGAGGCCGAGGCCGCGGCCGAGGAAAAGCAACTGGCGGCAGCAGCGG- CGG CGGCGGCGTTGGCCGCGGCGGAGGCTGAGTCTGAGGGGACCAAGCCCGCCAAGGAGCCCGTGATTAAGCCCCTG- ACC GAAGATAGCAAGAAGCGCAGTTACAACCTGCTCAAGGACAGCACCAACACCGCGTACCGCAGCTGGTACCTGGC- CTA CAACTACGGCGACCCGTCGACGGGGGTGCGCTCCTGGACCCTGCTGTGCACGCCGGACGTGACCTGCGGCTCGG- AGC AGGTGTACTGGTCGCTGCCCGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGCGGCAGGTCAGCAACTTC- CCG GTGGTGGGCGCCGAGCTGCTGCCCGTGCACTCCAAGAGCTTCTACAACGACCAGGCCGTCTACTCCCAGCTCAT- CCG CCAGTTCACCTCTCTGACCCACGTGTTCAATCGCTTTCCTGAGAACCAGATTCTGGCGCGCCCGCCCGCCCCCA- CCA TCACCACCGTCAGTGAAAACGTTCCTGCTCTCACAGATCACGGGACGCTACCGCTGCGCAACAGCATCGGAGGA- GTC CAGCGAGTGACCGTTACTGACGCCAGACGCCGCACCTGCCCCTACGTTTACAAGGCCTTGGGCATAGTCTCGCC- GCG CGTCCTTTCCAGCCGCACTTTTTGAGCAACACCACCATCATGTCCATCCTGATCTCACCCAGCAATAACTCCGG- CTG GGGACTGCTGCGCGCGCCCAGCAAGATGTTCGGAGGGGCGAGGAAGCGTTCCGAGCAGCACCCCGTGCGCGTGC- GCG GGCACTTCCGCGCCCCCTGGGGAGCGCACAAACGCGGCCGCGCGGGGCGCACCACCGTGGACGACGCCATCGAC- TCG GTGGTGGAGCAGGCGCGCAACTACAGGCCCGCGGTCTCTACCGTGGACGCGGCCATCCAGACCGTGGTGCGGGG- CGC GCGGCGGTACGCCAAGCTGAAGAGCCGCCGGAAGCGCGTGGCCCGCCGCCACCGCCGCCGACCCGGGGCCGCCG- CCA AACGCGCCGCCGCGGCCCTGCTTCGCCGGGCCAAGCGCACGGGCCGCCGCGCCGCCATGAGGGCCGCGCGCCGC- TTG GCCGCCGGCATCACCGCCGCCACCATGGCCCCCCGTACCCGAAGACGCGCGGCCGCCGCCGCCGCCGCCGCCAT- CAG TGACATGGCCAGCAGGCGCCGGGGCAACGTGTACTGGGTGCGCGACTCGGTGACCGGCACGCGCGTGCCCGTGC- GCT TCCGCCCCCCGCGGACTTGAGATGATGTGAAAAAACAACACTGAGTCTCCTGCTGTTGTGTGTATCCCAGCGGC- GGC GGCGCGCGCAGCGTCATGTCCAAGCGCAAAATCAAAGAAGAGATGCTCCAGGTCGTCGCGCCGGAGATCTATGG- GCC CCCGAAGAAGGAAGAGCAGGATTCGAAGCCCCGCAAGATAAAGCGGGTCAAAAAGAAAAAGAAAGATGATGACG- ATG CCGATGGGGAGGTGGAGTTCCTGCGCGCCACGGCGCCCAGGCGCCCGGTGCAGTGGAAGGGCCGGCGCGTAAAG- CGC GTCCTGCGCCCCGGCACCGCGGTGGTCTTCACGCCCGGCGAGCGCTCCACCCGGACTTTCAAGCGCGTCTATGA- CGA GGTGTACGGCGACGAAGACCTGCTGGAGCAGGCCAACGAGCGCTTCGGAGAGTTTGCTTACGGGAAGCGTCAGC- GGG CGCTGGGGAAGGAGGACCTGCTGGCGCTGCCGCTGGACCAGGGCAACCCCACCCCCAGTCTGAAGCCCGTGACC- CTG CAGCAGGTGCTGCCGAGCAGCGCACCCTCCGAGGCGAAGCGGGGTCTGAAGCGCGAGGGCGGCGACCTGGCGCC- CAC CGTGCAGCTCATGGTGCCCAAGCGGCAGAGGCTGGAGGATGTGCTGGAGAAAATGAAAGTAGACCCCGGTCTGC- AGC CGGACATCAGGGTCCGCCCCATCAAGCAGGTGGCGCCGGGCCTCGGCGTGCAGACCGTGGACGTGGTCATCCCC- ACC GGCAACTCCCCCGCCGCCGCCACCACTACCGCTGCCTCCACGGACATGGAGACACAGACCGATCCCGCCGCAGC- CGC AGCCGCAGCCGCCGCCGCGACCTCCTCGGCGGAGGTGCAGACGGACCCCTGGCTGCCGCCGGCGATGTCAGCTC- CCC GCGCGCGTCGCGGGCGCAGGAAGTACGGCGCCGCCAACGCGCTCCTGCCCGAGTACGCCTTGCATCCTTCCATC- GCG CCCACCCCCGGCTACCGAGGCTATACCTACCGCCCGCGAAGAGCCAAGGGTTCCACCCGCCGTCCCCGCCGACG- CGC CGCCGCCACCACCCGCCGCCGCCGCCGCAGACGCCAGCCCGCACTGGCTCCAGTCTCCGTGAGGAAAGTGGCGC- GCG ACGGACACACCCTGGTGCTGCCCAGGGCGCGCTACCACCCCAGCATCGTTTAAAAGCCTGTTGTGGTTCTTGCA- GAT ATGGCCCTCACTTGCCGCCTCCGTTTCCCGGTGCCGGGATACCGAGGAGGAAGATCGCGCCGCAGGAGGGGTCT- GGC CGGCCGCGGCCTGAGCGGAGGCAGCCGCCGCGCGCACCGGCGGCGACGCGCCACCAGCCGACGCATGCGCGGCG- GGG TGCTGCCCCTGTTAATCCCCCTGATCGCCGCGGCGATCGGCGCCGTGCCCGGGATCGCCTCCGTGGCCTTGCAA- GCG TCCCAGAGGCATTGACAGACTTGCAAACTTGCAAATATGGAAAAAAAAACCCCAATAAAAAAGTCTAGACTCTC- ACG CTCGCTTGGTCCTGTGACTATTTTGTAGAATGGAAGACATCAACTTTGCGTCGCTGGCCCCGCGTCACGGCTCG- CGC CCGTTCCTGGGACACTGGAACGATATCGGCACCAGCAACATGAGCGGTGGCGCCTTCAGTTGGGGCTCTCTGTG- GAG CGGCATTAAAAGTATCGGGTCTGCCGTTAAAAATTACGGCTCCCGGGCCTGGAACAGCAGCACGGGCCAGATGT- TGA GAGACAAGTTGAAAGAGCAGAACTTCCAGCAGAAGGTGGTGGAGGGCCTGGCCTCCGGCATCAACGGGGTGGTG- GAC CTGGCCAACCAGGCCGTGCAGAATAAGATCAACAGCAGACTGGACCCCCGGCCGCCGGTGGAGGAGGTGCCGCC- GGC GCTGGAGACGGTGTCCCCCGATGGGCGTGGCGAGAAGCGCCCGCGGCCCGATAGGGAAGAGACCACTCTGGTCA- CGC AGACCGATGAGCCGCCCCCGTATGAGGAGGCCCTGAAGCAAGGTCTGCCCACCACGCGGCCCATCGCGCCCATG- GCC ACCGGGGTGGTGGGCCGCCACACCCCCGCCACGCTGGACTTGCCTCCGCCCGCCGATGTGCCGCAGCAGCAGAA- GGC GGCACAGCCGGGCCCGCCCGCGACCGCCTCCCGTTCCTCCGCCGGTCCTCTGCGCCGCGCGGCCAGCGGCCCCC- GCG GGGGGGTCGCGAGGCACGGCAACTGGCAGAGCACGCTGAACAGCATCGTGGGTCTGGGGGTGCGGTCCGTGAAG- CGC CGCCGATGCTACTGAATAGCTTAGCTAACGTGTTGTATGTGTGTATGCGCCCTATGTCGCCGCCAGAGGAGCTG- CTG AGTCGCCGCCGTTCGCGCGCCCACCACCACCGCCACTCCGCCCCTCAAGATGGCGACCCCATCGATGATGCCGC- AGT GGTCGTACATGCACATCTCGGGCCAGGACGCCTCGGAGTACCTGAGCCCCGGGCTGGTGCAGTTCGCCCGCGCC- ACC GAGAGCTACTTCAGCCTGAGTAACAAGTTTAGGAACCCCACGGTGGCGCCCACGCACGATGTGACCACCGACCG- GTC

TCAGCGCCTGACGCTGCGGTTCATTCCCGTGGACCGCGAGGACACCGCGTACTCGTACAAGGCGCGGTTCACCC- TGG CCGTGGGCGACAACCGCGTGCTGGACATGGCCTCCACCTACTTTGACATCCGCGGGGTGCTGGACCGGGGTCCC- ACT TTCAAGCCCTACTCTGGCACCGCCTACAACTCCCTGGCCCCCAAGGGCGCTCCCAACTCCTGCGAGTGGGAGCA- AGA GGAAACTCAGGCAGTTGAAGAAGCAGCAGAAGAGGAAGAAGAAGATGCTGACGGTCAAGCTGAGGAAGAGCAAG- CAG CTACCAAAAAGACTCATGTATATGCTCAGGCTCCCCTTTCTGGCGAAAAAATTAGTAAAGATGGTCTGCAAATA- GGA ACGGACGCTACAGCTACAGAACAAAAACCTATTTATGCAGACCCTACATTCCAGCCCGAACCCCAAATCGGGGA- GTC CCAGTGGAATGAGGCAGATGCTACAGTCGCCGGCGGTAGAGTGCTAAAGAAATCTACTCCCATGAAACCATGCT- ATG GTTCCTATGCAAGACCCACAAATGCTAATGGAGGTCAGGGTGTACTAACGGCAAATGCCCAGGGACAGCTAGAA- TCT CAGGTTGAAATGCAATTCTTTTCAACTTCTGAAAACGCCCGTAACGAGGCTAACAACATTCAGCCCAAATTGGT- GCT GTATAGTGAGGATGTGCACATGGAGACCCCGGATACGCACCTTTCTTACAAGCCCGCAAAAAGCGATGACAATT- CAA AAATCATGCTGGGTCAGCAGTCCATGCCCAACAGACCTAATTACATCGGCTTCAGAGACAACTTTATCGGCCTC- ATG TATTACAATAGCACTGGCAACATGGGAGTGCTTGCAGGTCAGGCCTCTCAGTTGAATGCAGTGGTGGACTTGCA- AGA CAGAAACACAGAACTGTCCTACCAGCTCTTGCTTGATTCCATGGGTGACAGAACCAGATACTTTTCCATGTGGA- ATC AGGCAGTGGACAGTTATGACCCAGATGTTAGAATTATTGAAAATCATGGAACTGAAGACGAGCTCCCCAACTAT- TGT TTCCCTCTGGGTGGCATAGGGGTAACTGACACTTACCAGGCTGTTAAAACCAACAATGGCAATAACGGGGGCCA- GGT GACTTGGACAAAAGATGAAACTTTTGCAGATCGCAATGAAATAGGGGTGGGAAACAATTTCGCTATGGAGATCA- ACC TCAGTGCCAACCTGTGGAGAAACTTCCTGTACTCCAACGTGGCGCTGTACCTACCAGACAAGCTTAAGTACAAC- CCC TCCAATGTGGACATCTCTGACAACCCCAACACCTACGATTACATGAACAAGCGAGTGGTGGCCCCGGGGCTGGT- GGA CTGCTACATCAACCTGGGCGCGCGCTGGTCGCTGGACTACATGGACAACGTCAACCCCTTCAACCACCACCGCA- ATG CGGGCCTGCGCTACCGCTCCATGCTCCTGGGCAACGGGCGCTACGTGCCCTTCCACATCCAGGTGCCCCAGAAG- TTC TTTGCCATCAAGAACCTCCTCCTCCTGCCGGGCTCCTACACCTACGAGTGGAACTTCAGGAAGGATGTCAACAT- GGT CCTCCAGAGCTCTCTGGGTAACGATCTCAGGGTGGACGGGGCCAGCATCAAGTTCGAGAGCATCTGCCTCTACG- CCA CCTTCTTCCCCATGGCCCACAACACGGCCTCCACGCTCGAGGCCATGCTCAGGAACGACACCAACGACCAGTCC- TTC AATGACTACCTCTCCGCCGCCAACATGCTCTACCCCATACCCGCCAACGCCACCAACGTCCCCATCTCCATCCC- CTC GCGCAACTGGGCGGCCTTCCGCGGCTGGGCCTTCACCCGCCTCAAGACCAAGGAGACCCCCTCCCTGGGCTCGG- GAT TCGACCCCTACTACACCTACTCGGGCTCCATTCCCTACCTGGACGGCACCTTCTACCTCAACCACACTTTCAAG- AAG GTCTCGGTCACCTTCGACTCCTCGGTCAGCTGGCCGGGCAACGACCGTCTGCTCACCCCCAACGAGTTCGAGAT- CAA GCGCTCGGTCGACGGGGAGGGCTACAACGTGGCCCAGTGCAACATGACCAAGGACTGGTTCCTGGTCCAGATGC- TGG CCAACTACAACATCGGCTACCAGGGCTTCTACATCCCAGAGAGCTACAAGGACAGGATGTACTCCTTCTTCAGG- AAC TTCCAGCCCATGAGCCGGCAGGTGGTGGACCAGACCAAGTACAAGGACTACCAGGAGGTGGGCATCATCCACCA- GCA CAACAACTCGGGCTTCGTGGGCTACCTCGCCCCCACCATGCGCGAGGGACAGGCCTACCCCGCCAACTTCCCCT- ATC CGCTCATAGGCAAGACCGCGGTCGACAGCATCACCCAGAAAAAGTTCCTCTGCGACCGCACCCTCTGGCGCATC- CCC TTCTCCAGCAACTTCATGTCCATGGGTGCGCTCTCGGACCTGGGCCAGAACTTGCTCTACGCCAACTCCGCCCA- CGC CCTCGACATGACCTTCGAGGTCGACCCCATGGACGAGCCCACCCTTCTCTATGTTCTGTTCGAAGTCTTTGACG- TGG TCCGGGTCCACCAGCCGCACCGCGGCGTCATCGAGACCGTGTACCTGCGTACGCCCTTCTCGGCCGGCAACGCC- ACC ACCTAAAGAAGCAAGCCGCAGTCATCGCCGCCTGCATGCCGTCGGGTTCCACCGAGCAAGAGCTCAGGGCCATC- GTC AGAGACCTGGGATGCGGGCCCTATTTTTTGGGCACCTTCGACAAGCGCTTCCCTGGCTTTGTCTCCCCACACAA- GCT GGCCTGCGCCATCGTCAACACGGCCGGCCGCGAGACCGGGGGCGTGCACTGGCTGGCCTTCGCCTGGAACCCGC- GCT CCAAAACATGCTTCCTCTTTGACCCCTTCGGCTTTTCGGACCAGCGGCTCAAGCAAATCTACGAGTTCGAGTAC- GAG GGCTTGCTGCGTCGCAGCGCCATCGCCTCCTCGCCCGACCGCTGCGTCACCCTCGAAAAGTCCACCCAGACCGT- GCA GGGGCCCGACTCGGCCGCCTGCGGTCTCTTCTGCTGCATGTTTCTGCACGCCTTTGTGCACTGGCCTCAGAGTC- CCA TGGACCGCAACCCCACCATGAACTTGCTGACGGGGGTGCCCAACTCCATGCTCCAGAGCCCCCAGGTCGAGCCC- ACC CTGCGCCGCAACCAGGAGCAGCTCTACAGCTTCCTGGAGCGCCACTCGCCTTACTTCCGCCGCCACAGCGCACA- GAT CAGGAGGGCCACCTCCTTCTGCCACTTGCAAGAGATGCAAGAAGGGTAATAACGATGTACACACTTTTTTTCTC- AAT AAATGGCATCTTTTTATTTATACAAGCTCTCTGGGGTATTCATTTCCCACCACCACCCGCCGTTGTCGCCATCT- GGC TCTATTTAGAAATCGAAAGGGTTCTGCCGGGAGTCGCCGTGCGCCACGGGCAGGGACACGTTGCGATACTGGTA- GCG GGTGCCCCACTTGAACTCGGGCACCACCAGGCGAGGCAGCTCGGGGAAGTTTTCGCTCCACAGGCTGCGGGTCA- GCA CCAGCGCGTTCATCAGGTCGGGCGCCGAGATCTTGAAGTCGCAGTTGGGGCCGCCGCCCTGCGCGCGCGAGTTG- CGG TACACCGGGTTGCAGCACTGGAACACCAACAGCGCCGGGTGCTTCACGCTGGCCAGCACGCTGCGGTCGGAGAT- CAG CTCGGCGTCCAGGTCCTCCGCGTTGCTCAGCGCGAACGGGGTCATCTTGGGCACTTGCCGCCCCAGGAAGGGCG- CGT GCCCCGGTTTCGAGTTGCAGTCGCAGCGCAGCGGGATCAGCAGGTGCCCGTGCCCGGACTCGGCGTTGGGGTAC- AGC GCGCGCATGAAGGCCTGCATCTGGCGGAAGGCCATCTGGGCCTTGGCGCCCTCCGAGAAGAACATGCCGCAGGA- CTT GCCCGAGAACTGGTTTGCGGGGCAGCTGGCGTCGTGCAGGCAGCAGCGCGCGTCGGTGTTGGCGATCTGCACCA- CGT TGCGCCCCCACCGGTTCTTCACGATCTTGGCCTTGGACGATTGCTCCTTCAGCGCGCGCTGCCCGTTCTCGCTG- GTC ACATCCATCTCGATCACATGTTCCTTGTTCACCATGCTGCTGCCGTGCAGACACTTCAGCTCGCCCTCCGTCTC- GGT GCAGCGGTGCTGCCACAGCGCGCAGCCCGTGGGCTCGAAAGACTTGTAGGTCACCTCCGCGAAGGACTGCAGGT- ACC CCTGCAAAAAGCGGCCCATCATGGTCACGAAGGTCTTGTTGCTGCTGAAGGTCAGCTGCAGCCCGCGGTGCTCC- TCG TTCAGCCAGGTCTTGCACACGGCCGCCAGCGCCTCCACCTGGTCGGGCAGCATCTTGAAGTTCACCTTCAGCTC- ATT CTCCACGTGGTACTTGTCCATCAGCGTGCGCGCCGCCTCCATGCCCTTCTCCCAGGCCGACACCAGCGGCAGGC- TCA CGGGGTTCTTCACCATCACCGTGGCCGCCGCCTCCGCCGCGCTTTCGCTTTCCGCCCCGCTGTTCTCTTCCTCT- TCC TCCTCTTCCTCGCCGCCGCCCACTCGCAGCCCCCGCACCACGGGGTCGTCTTCCTGCAGGCGCTGCACCTTGCG- CTT GCCGTTGCGCCCCTGCTTGATGCGCACGGGCGGGTTGCTGAAGCCCACCATCACCAGCGCGGCCTCTTCTTGCT- CGT CCTCGCTGTCCAGAATGACCTCCGGGGAGGGGGGGTTGGTCATCCTCAGTACCGAGGCACGCTTCTTTTTCTTC- CTG GGGGCGTTCGCCAGCTCCGCGGCTGCGGCCGCTGCCGAGGTCGAAGGCCGAGGGCTGGGCGTGCGCGGCACCAG- CGC GTCCTGCGAGCCGTCCTCGTCCTCCTCGGACTCGAGACGGAGGCGGGCCCGCTTCTTCGGGGGCGCGCGGGGCG- GCG GAGGCGGCGGCGGCGACGGAGACGGGGACGAGACATCGTCCAGGGTGGGTGGACGGCGGGCCGCGCCGCGTCCG- CGC TCGGGGGTGGTCTCGCGCTGGTCCTCTTCCCGACTGGCCATCTCCCACTGCTCCTTCTCCTATAGGCAGAAAGA- GAT CATGGAGTCTCTCATGCGAGTCGAGAAGGAGGAGGACAGCCTAACCGCCCCCTCTGAGCCCTCCACCACCGCCG- CCA CCACCGCCAATGCCGCCGCGGACGACGCGCCCACCGAGACCACCGCCAGTACCACCCTCCCCAGCGACGCACCC- CCG CTCGAGAATGAAGTGCTGATCGAGCAGGACCCGGGTTTTGTGAGCGGAGAGGAGGATGAGGTGGATGAGAAGGA- GAA GGAGGAGGTCGCCGCCTCAGTGCCAAAAGAGGATAAAAAGCAAGACCAGGACGACGCAGATAAGGATGAGACAG- CAG TCGGGCGGGGGAACGGAAGCCATGATGCTGATGACGGCTACCTAGACGTGGGAGACGACGTGCTGCTTAAGCAC- CTG CACCGCCAGTGCGTCATCGTCTGCGACGCGCTGCAGGAGCGCTGCGAAGTGCCCCTGGACGTGGCGGAGGTCAG- CCG CGCCTACGAGCGGCACCTCTTCGCGCCGCACGTGCCCCCCAAGCGCCGGGAGAACGGCACCTGCGAGCCCAACC- CGC GTCTCAACTTCTACCCGGTCTTCGCGGTACCCGAGGTGCTGGCCACCTACCACATCTTTTTCCAAAACTGCAAG- ATC CCCCTCTCCTGCCGCGCCAACCGCACCCGCGCCGACAAAACCCTGACCCTGCGGCAGGGCGCCCACATACCTGA- TAT CGCCTCTCTGGAGGAAGTGCCCAAGATCTTCGAGGGTCTCGGTCGCGACGAGAAACGGGCGGCGAACGCTCTGC- ACG GAGACAGCGAAAACGAGAGTCACTCGGGGGTGCTGGTGGAGCTCGAGGGCGACAACGCGCGCCTGGCCGTACTC- AAG CGCAGCATAGAGGTCACCCACTTTGCCTACCCGGCGCTCAACCTGCCCCCCAAGGTCATGAGTGTGGTCATGGG- CGA GCTCATCATGCGCCGCGCCCAGCCCCTGGCCGCGGATGCAAACTTGCAAGAGTCCTCCGAGGAAGGCCTGCCCG- CGG TCAGCGACGAGCAGCTGGCGCGCTGGCTGGAGACCCGCGACCCCGCGCAGCTGGAGGAGCGGCGCAAGCTCATG- ATG GCCGCGGTGCTGGTCACCGTGGAGCTCGAGTGTCTGCAGCGCTTCTTCGCGGACCCCGAGATGCAGCGCAAGCT- CGA GGAGACCCTGCACTACACCTTCCGCCAGGGCTACGTGCGCCAGGCCTGCAAGATCTCCAACGTGGAGCTCTGCA- ACC TGGTCTCCTACCTGGGCATCCTGCACGAGAACCGCCTCGGGCAGAACGTCCTGCACTCCACCCTCAAAGGGGAG- GCG

CGCCGCGACTACATCCGCGACTGCGCCTACCTCTTCCTCTGCTACACCTGGCAGACGGCCATGGGGGTCTGGCA- GCA GTGCCTGGAGGAGCGCAACCTCAAGGAGCTGGAAAAGCTCCTCAAGCGCACCCTCAGGGACCTCTGGACGGGCT- TCA ACGAGCGCTCGGTGGCCGCCGCGCTGGCGGACATCATCTTTCCCGAGCGCCTGCTCAAGACCCTGCAGCAGGGC- CTG CCCGACTTCACCAGCCAGAGCATGCTGCAGAACTTCAGGACTTTCATCCTGGAGCGCTCGGGCATCCTGCCGGC- CAC TTGCTGCGCGCTGCCCAGCGACTTCGTGCCCATCAAGTACAGGGAGTGCCCGCCGCCGCTCTGGGGCCACTGCT- ACC TCTTCCAGCTGGCCAACTACCTCGCCTACCACTCGGACCTCATGGAAGACGTGAGCGGCGAGGGCCTGCTCGAG- TGC CACTGCCGCTGCAACCTCTGCACGCCCCACCGCTCTCTAGTCTGCAACCCGCAGCTGCTCAGCGAGAGTCAGAT- TAT CGGTACCTTCGAGCTGCAGGGTCCCTCGCCTGACGAGAAGTCCGCGGCTCCAGGGCTGAAACTCACTCCGGGGC- TGT GGACTTCCGCCTACCTACGCAAATTTGTACCTGAGGACTACCACGCCCACGAGATCAGGTTCTACGAAGACCAA- TCC CGCCCGCCCAAGGCGGAGCTCACCGCCTGCGTCATCACCCAGGGGCACATCCTGGGCCAATTGCAAGCCATCAA- CAA AGCCCGCCGAGAGTTCTTGCTGAAAAAGGGTCGGGGGGTGTACCTGGACCCCCAGTCCGGCGAGGAGCTAAACC- CGC TACCCCCGCCGCCGCCCCAGCAGCGGGACCTTGCTTCCCAGGATGGCACCCAGAAAGAAGCAGCAGCCGCCGCC- GCC GCCGCAGCCATACATGCTTCTGGAGGAAGAGGAGGAGGACTGGGACAGTCAGGCAGAGGAGGTTTCGGACGAGG- AGC AGGAGGAGATGATGGAAGACTGGGAGGAGGACAGCAGCCTAGACGAGGAAGCTTCAGAGGCCGAAGAGGTGGCA- GAC GCAACACCATCGCCCTCGGTCGCAGCCCCCTCGCCGGGGCCCCTGAAATCCTCCGAACCCAGCACCAGCGCTAT- AAC CTCCGCTCCTCCGGCGCCGGCGCCACCCGCCCGCAGACCCAACCGTAGATGGGACACCACAGGAACCGGGGTCG- GTA AGTCCAAGTGCCCGCCGCCGCCACCGCAGCAGCAGCAGCAGCAGCGCCAGGGCTACCGCTCGTGGCGCGGGCAC- AAG AACGCCATAGTCGCCTGCTTGCAAGACTGCGGGGGCAACATCTCTTTCGCCCGCCGCTTCCTGCTATTCCACCA- CGG GGTCGCCTTTCCCCGCAATGTCCTGCATTACTACCGTCATCTCTACAGCCCCTACTGCAGCGGCGACCCAGAGG- CGG CAGCGGCAGCCACAGCGGCGACCACCACCTAGGAAGATATCCTCCGCGGGCAAGACAGCGGCAGCAGCGGCCAG- GAG ACCCGCGGCAGCAGCGGCGGGAGCGGTGGGCGCACTGCGCCTCTCGCCCAACGAACCCCTCTCGACCCGGGAGC- TCA GACACAGGATCTTCCCCACTTTGTATGCCATCTTCCAACAGAGCAGAGGCCAGGAGCAGGAGCTGAAAATAAAA- AAC AGATCTCTGCGCTCCCTCACCCGCAGCTGTCTGTATCACAAAAGCGAAGATCAGCTTCGGCGCACGCTGGAGGA- CGC GGAGGCACTCTTCAGCAAATACTGCGCGCTCACTCTTAAAGACTAGCTCCGCGCCCTTCTCGAATTTAGGCGGG- AGA AAACTACGTCATCGCCGGCCGCCGCCCAGCCCGCCCAGCCGAGATGAGCAAAGAGATTCCCACGCCATACATGT- GGA GCTACCAGCCGCAGATGGGACTCGCGGCGGGAGCGGCCCAGGACTACTCCACCCGCATGAACTACATGAGCGCG- GGA CCCCACATGATCTCACAGGTCAACGGGATCCGCGCCCAGCGAAACCAAATACTGCTGGAACAGGCGGCCATCAC- CGC CACGCCCCGCCATAATCTCAACCCCCGAAATTGGCCCGCCGCCCTCGTGTACCAGGAAACCCCCTCCGCCACCA- CCG TACTACTTCCGCGTGACGCCCAGGCCGAAGTCCAGATGACTAACTCAGGGGCGCAGCTCGCGGGCGGCTTTCGT- CAC GGGGCGCGGCCGCTCCGACCAGGTATAAGACACCTGATGATCAGAGGCCGAGGTATCCAGCTCAACGACGAGTC- GGT GAGCTCTTCGCTCGGTCTCCGTCCGGACGGAACTTTCCAGCTCGCCGGATCCGGCCGCTCTTCGTTCACGCCCC- GCC AGGCGTACCTGACTCTGCAGACCTCGTCCTCGGAGCCCCGCTCCGGCGGCATCGGAACCCTCCAGTTCGTGGAG- GAG TTCGTGCCCTCGGTCTACTTCAACCCCTTCTCGGGACCTCCCGGACGCTACCCCGACCAGTTCATTCCGAACTT- TGA CGCGGTGAAGGACTCGGCGGACGGCTACGACTGAATGTCAGGTGTCGAGGCAGAGCAGCTTCGCCTGAGACACC- TCG AGCACTGCCGCCGCCACAAGTGCTTCGCCCGCGGTTCTGGTGAGTTCTGCTACTTTCAGCTACCCGAGGAGCAT- ACC GAGGGGCCGGCGCACGGCGTCCGCCTGACCACCCAGGGCGAGGTTACCTGTTCCCTCATCCGGGAGTTTACCCT- CCG TCCCCTGCTAGTGGAGCGGGAGCGGGGTCCCTGTGTCCTAACTATCGCCTGCAACTGCCCTAACCCTGGATTAC- ATC AAGATCTTTGCTGTCATCTCTGTGCTGAGTTTAATAAACGCTGAGATCAGAATCTACTGGGGCTCCTGTCGCCA- TCC TGTGAACGCCACCGTCTTCACCCACCCCGACCAGGCCCAGGCGAACCTCACCTGCGGTCTGCATCGGAGGGCCA- AGA AGTACCTCACCTGGTACTTCAACGGCACCCCCTTTGTGGTTTACAACAGCTTCGACGGGGACGGAGTCTCCCTG- AAA GACCAGCTCTCCGGTCTCAGCTACTCCATCCACAAGAACACCACCCTCCAACTCTTCCCTCCCTACCTGCCGGG- AAC CTACGAGTGCGTCACCGGCCGCTGCACCCACCTCACCCGCCTGATCGTAAACCAGAGCTTTCCGGGAACAGATA- ACT CCCTCTTCCCCAGAACAGGAGGTGAGCTCAGGAAACTCCCCGGGGACCAGGGCGGAGACGTACCTTCGACCCTT- GTG GGGTTAGGATTTTTTATTACCGGGTTGCTGGCTCTTTTAATCAAAGTTTCCTTGAGATTTGTTCTTTCCTTCTA- CGT GTATGAACACCTCAACCTCCAATAACTCTACCCTTTCTTCGGAATCAGGTGACTTCTCTGAAATCGGGCTTGGT- GTG CTGCTTACTCTGTTGATTTTTTTCCTTATCATACTCAGCCTTCTGTGCCTCAGGCTCGCCGCCTGCTGCGCACA- CAT CTATATCTACTGCTGGTTGCTCAAGTGCAGGGGTCGCCACCCAAGATGAACAGGTACATGGTCCTATCGATCCT- AGG CCTGCTGGCCCTGGCGGCCTGCAGCGCCGCCAAAAAAGAGATTACCTTTGAGGAGCCCGCTTGCAATGTAACTT- TCA AGCCCGAGGGTGACCAATGCACCACCCTCGTCAAATGCGTTACCAATCATGAGAGGCTGCGCATCGACTACAAA- AAC AAAACTGGCCAGTTTGCGGTCTATAGTGTGTTTACGCCCGGAGACCCCTCTAACTACTCTGTCACCGTCTTCCA- GGG CGGACAGTCTAAGATATTCAATTACACTTTCCCTTTTTATGAGTTATGCGATGCGGTCATGTACATGTCAAAAC- AGT ACAACCTGTGGCCTCCCTCTCCCCAGGCGTGTGTGGAAAATACTGGGTCTTACTGCTGTATGGCTTTCGCAATC- ACT ACGCTCGCTCTAATCTGCACGGTGCTATACATAAAATTCAGGCAGAGGCGAATCTTTATCGATGAAAAGAAAAT- GCC TTGATCGCTAACACCGGCTTTCTATCTGCAGAATGAATGCAATCACCTCCCTACTAATCACCACCACCCTCCTT- GCG ATTGCCCATGGGTTGACACGAATCGAAGTGCCAGTGGGGTCCAATGTCACCATGGTGGGCCCCGCCGGCAATTC- CAC CCTCATGTGGGAAAAATTTGTCCGCAATCAATGGGTTCATTTCTGCTCTAACCGAATCAGTATCAAGCCCAGAG- CCA TCTGCGATGGGCAAAATCTAACTCTGATCAATGTGCAAATGATGGATGCTGGGTACTATTACGGGCAGCGGGGA- GAA ATCATTAATTACTGGCGACCCCACAAGGACTACATGCTGCATGTAGTCGAGGCACTTCCCACTACCACCCCCAC- TAC CACCTCTCCCACCACCACCACCACTACTACTACTACTACTACTACTACTACTACTACCACTACCGCTGCCCGCC- ATA CCCGCAAAAGCACCATGATTAGCACAAAGCCCCCTCGTGCTCACTCCCACGCCGGCGGGCCCATCGGTGCGACC- TCA GAAACCACCGAGCTTTGCTTCTGCCAATGCACTAACGCCAGCGCTCATGAACTGTTCGACCTGGAGAATGAGGA- TGT CCAGCAGAGCTCCGCTTGCCTGACCCAGGAGGCTGTGGAGCCCGTTGCCCTGAAGCAGATCGGTGATTCAATAA- TTG ACTCTTCTTCTTTTGCCACTCCCGAATACCCTCCCGATTCTACTTTCCACATCACGGGTACCAAAGACCCTAAC- CTC TCTTTCTACCTGATGCTGCTGCTCTGTATCTCTGTGGTCTCTTCCGCGCTGATGTTACTGGGGATGTTCTGCTG- CCT GATCTGCCGCAGAAAGAGAAAAGCTCGCTCTCAGGGCCAACCACTGATGCCCTTCCCCTACCCCCCGGATTTTG- CAG ATAACAAGATATGAGCTCGCTGCTGACACTAACCGCTTTACTAGCCTGCGCTCTAACCCTTGTCGCTTGCGACT- CGA GATTCCACAATGTCACAGCTGTGGCAGGAGAAAATGTTACTTTCAACTCCACGGCCGATACCCAGTGGTCGTGG- AGT GGCTCAGGTAGCTACTTAACTATCTGCAATAGCTCCACTTCCCCCGGCATATCCCCAACCAAGTACCAATGCAA- TGC CAGCCTGTTCACCCTCATCAACGCTTCCACCCTGGACAATGGACTCTATGTAGGCTATGTACCCTTTGGTGGGC- AAG GAAAGACCCACGCTTACAACCTGGAAGTTCGCCAGCCCAGAACCACTACCCAAGCTTCTCCCACCACCACCACC- ACC ACCACCATCACCAGCAGCAGCAGCAGCAGCAGCCACAGCAGCAGCAGCAGATTATTGACTTTGGTTTTGGCCAG- CTC ATCTGCCGCTACCCAGGCCATCTACAGCTCTGTGCCCGAAACCACTCAGATCCACCGCCCAGAAACGACCACCG- CCA CCACCCTACACACCTCCAGCGATCAGATGCCGACCAACATCACCCCCTTGGCTCTTCAAATGGGACTTACAAGC- CCC ACTCCAAAACCAGTGGATGCGGCCGAGGTCTCCGCCCTCGTCAATGACTGGGCGGGGCTGGGAATGTGGTGGTT- CGC CATAGGCATGATGGCGCTCTGCCTGCTTCTGCTCTGGCTCATCTGCTGCCTCCACCGCAGGCGAGCCAGACCCC- CCA TCTATAGACCCATCATTGTCCTGAACCCCGATAATGATGGGATCCATAGATTGGATGGCCTGAAAAACCTACTT- TTT TCTTTTACAGTATGATAAATTGAGACATGCCTCGCATTTTCTTGTACATGTTCCTTCTCCCACCTTTTCTGGGG- TGT TCTACGCTGGCCGCTGTGTCTCACCTGGAGGTAGACTGCCTCTCACCCTTCACTGTCTACCTGCTTTACGGATT- GGT CACCCTCACTCTCATCTGCAGCCTAATCACAGTAATCATCGCCTTCATCCAGTGCATTGATTACATCTGTGTGC- GCC TCGCATACTTCAGACACCACCCGCAGTACCGAGACAGGAACATTGCCCAACTTCTAAGACTGCTCTAATCATGC- ATA AGACTGTGATCTGCCTTCTGATCCTCTGCATCCTGCCCACCCTCACCTCCTGCCAGTACACCACAAAATCTCCG- CGC AAAAGACATGCCTCCTGCCGCTTCACCCAACTGTGGAATATACCCAAATGCTACAACGAAAAGAGCGAGCTCTC- CGA AGCTTGGCTGTATGGGGTCATCTGTGTCTTAGTTTTCTGCAGCACTGTCTTTGCCCTCATAATCTACCCCTACT- TTG ATTTGGGATGGAACGCGATCGATGCCATGAATTACCCCACCTTTCCCGCACCCGAGATAATTCCACTGCGACAA-

GTT GTACCCGTTGTCGTTAATCAACGCCCCCCATCCCCTACGCCCACTGAAATCAGCTACTTTAACCTAACAGGCGG- AGA TGACTGACGCCCTAGATCTAGAAATGGACGGCATCAGTACCGAGCAGCGTCTCCTAGAGAGGCGCAGGCAGGCG- GCT GAGCAAGAGCGCCTCAATCAGGAGCTCCGAGATCTCGTTAACCTGCACCAGTGCAAAAGAGGCATCTTTTGTCT- GGT AAAGCAGGCCAAAGTCACCTACGAGAAGACCGGCAACAGCCACCGCCTCAGTTACAAATTGCCCACCCAGCGCC- AGA AGCTGGTGCTCATGGTGGGTGAGAATCCCATCACCGTCACCCAGCACTCGGTAGAGACCGAGGGGTGTCTGCAC- TCC CCCTGTCGGGGTCCAGAAGACCTCTGCACCCTGGTAAAGACCCTGTGCGGTCTCAGAGATTTAGTCCCCTTTAA- CTA ATCAAACACTGGAATCAATAAAAAGAATCACTTACTTAAAATCAGACAGCAGGTCTCTGTCCAGTTTATTCAGC- AGC ACCTCCTTCCCCTCCTCCCAACTCTGGTACTCCAAACGCCTTCTGGCGGCAAACTTCCTCCACACCCTGAAGGG- AAT GTCAGATTCTTGCTCCTGTCCCTCCGCACCCACTATCTTCATGTTGTTGCAGATGAAGCGCACCAAAACGTCTG- ACG AGAGCTTCAACCCCGTGTACCCCTATGACACGGAAAGCGGCCCTCCCTCCGTCCCTTTCCTCACCCCTCCCTTC- GTG TCTCCCGATGGATTCCAAGAAAGTCCCCCCGGGGTCCTGTCTCTGAACCTGGCCGAGCCCCTGGTCACTTCCCA- CGG CATGCTCGCCCTGAAAATGGGAAGTGGCCTCTCCCTGGACGACGCTGGCAACCTCACCTCTCAAGATATCACCA- CCG CTAGCCCTCCCCTCAAAAAAACCAAGACCAACCTCAGCCTAGAAACCTCATCCCCCCTAACTGTGAGCACCTCA- GGC GCCCTCACCGTAGCAGCCGCCGCTCCCCTGGCGGTGGCCGGCACCTCCCTCACCATGCAATCAGAGGCCCCCCT- GAC AGTACAGGATGCAAAACTCACCCTGGCCACCAAAGGCCCCCTGACCGTGTCTGAAGGCAAACTGGCCTTGCAAA- CAT CGGCCCCGCTGACGGCCGCTGACAGCAGCACCCTCACAGTCAGTGCCACACCACCCCTTAGCACAAGCAATGGC- AGC TTGGGTATTGACATGCAAGCCCCCATTTACACCACCAATGGAAAACTAGGACTTAACTTTGGCGCTCCCCTGCA- TGT GGTAGACAGCCTAAATGCACTGACTGTAGTTACTGGCCAAGGTCTTACGATAAACGGAACAGCCCTACAAACTA- GAG TCTCAGGTGCCCTCAACTATGACACATCAGGAAACCTAGAATTGAGAGCTGCAGGGGGTATGCGAGTTGATGCA- AAT GGTCAACTTATCCTTGATGTAGCTTACCCATTTGATGCACAAAACAATCTCAGCCTTAGGCTTGGACAGGGACC- CCT GTTTGTTAACTCTGCCCACAACTTGGATGTTAACTACAACAGAGGCCTCTACCTGTTCACATCTGGAAATACCA- AAA AGCTAGAAGTTAATATCAAAACAGCCAAGGGTCTCATTTATGATGACACTGCTATAGCAATCAATGCGGGTGAT- GGG CTACAGTTTGACTCAGGCTCAGATACAAATCCATTAAAAACTAAACTTGGATTAGGACTGGATTATGACTCCAG- CAG AGCCATAATTGCTAAACTGGGAACTGGCCTAAGCTTTGACAACACAGGTGCCATCACAGTAGGCAACAAAAATG- ATG ACAAGCTTACCTTGTGGACCACACCAGACCCATCCCCTAACTGTAGAATCTATTCAGAGAAAGATGCTAAATTC- ACA CTTGTTTTGACTAAATGCGGCAGTCAGGTGTTGGCCAGCGTTTCTGTTTTATCTGTAAAAGGTAGCCTTGCGCC- CAT CAGTGGCACAGTAACTAGTGCTCAGATTGTCCTCAGATTTGATGAAAATGGAGTTCTACTAAGCAATTCTTCCC- TTG ACCCTCAATACTGGAACTACAGAAAAGGTGACCTTACAGAGGGCACTGCATATACCAACGCAGTGGGATTTATG- CCC AACCTCACAGCATACCCAAAAACACAGAGCCAAACTGCTAAAAGCAACATTGTAAGTCAGGTTTACTTGAATGG- GGA CAAATCCAAACCCATGACCCTCACCATTACCCTCAATGGAACTAATGAAACAGGAGATGCCACAGTAAGCACTT- ACT CCATGTCATTCTCATGGAACTGGAATGGAAGTAATTACATTAATGAAACGTTCCAAACCAACTCCTTCACCTTC- TCC TACATCGCCCAAGAATAAAAAGCATGACGCTGTTGATTTGATTCAATGTGTTTCTGTTTTATTTTCAAGCACAA- CAA AATCATTCAAGTCATTCTTCCATCTTAGCTTAATAGACACAGTAGCTTAATAGACCCAGTAGTGCAAAGCCCCA- TTC TAGCTTATAGATCAGACAGTGATAATTAACCACCACCACCACCATACCTTTTGATTCAGGAAATCATGATCATC- ACA GGATCCTAGTCTTCAGGCCGCCCCCTCCCTCCCAAGACACAGAATACACAGTCCTCTCCCCCCGACTGGCTTTA- AAT AACACCATCTGGTTGGTCACAGACATGTTCTTAGGGGTTATATTCCACACGGTCTCCTGCCGCGCCAGGCGCTC- GTC GGTGATGTTGATAAACTCTCCCGGCAGCTCGCTCAAGTTCACGTCGCTGTCCAGCGGCTGAACCTCCGGCTGAC- GCG ATAACTGTGCGACCGGCTGCTGGACGAACGGAGGCCGCGCCTACAAGGGGGTAGAGTCATAATCCTCGGTCAGG- ATA GGGCGGTGATGCAGCAGCAGCGAGCGAAACATCTGCTGCCGCCGCCGCTCCGTCCGGCAGGAAAACAACACGCC- GGT GGTCTCCTCCGCGATAATCCGCACCGCCCGCAGCATCAGCTTCCTCGTTCTCCGCGCGCAGCACCTCACCCTTA- TCT CGCTCAAATCGGCGCAGTAGGTACAGCACAGCACCACGATGTTATTCATGATCCCACAGTGCAGGGCGCTGTAT- CCA AAGCTCATGCCGGGAACCACCGCCCCCACGTGGCCATCGTACCACAAGCGCACGTAAATCAAGTGTCGACCCCT- CAT GAACGCGCTGGACACAAACATTACTTCCTTGGGCATGTTGTAATTCACCACCTCCCGGTACCAGATAAACCTCT- GGT TGAACAGGGCACCTTCCACCACCATCCTGAACCAAGAGGCCAGAACCTGCCCACCGGCTATGCACTGCAGGGAA- CCC GGGTTGGAACAATGACAATGCAGACTCCAAGGCTCGTAACCGTGGATCATCCGGCTGCTGAAGGCATCGATGTT- GGC ACAACACAGACACACGTGCATGCACTTTCTCATGATTAGCAGCTCTTCCCTCGTCAGGATCATATCCCAAGGAA- TAA CCCATTCTTGAATCAACGTAAAACCCACACAGCAGGGAAGGCCTCGCACATAACTCACGTTGTGCATGGTCAGC- GTG TTGCATTCCGGAAACAGCGGATGATCCTCCAGTATCGAGGCGCGGGTCTCCTTCTCACAGGGAGGTAAAGGGTC- CCT GCTGTACGGACTGCGCCGGGACGACCGAGATCGTGTTGAGCGTAGTGTCATGGAAAAGGGAACGCCGGACGTGG- TCA TACTTCTTGAAGCAGAACCAGGTTCGCGCGTGGCAGGCCTCCTTGCGTCTGCGGTCTCGCCGTCTAGCTCGCTC- CGT GTGATAGTTGTAGTACAGCCACTCCCGCAGAGCGTCGAGGCGCACCCTGGCTTCCGGATCTATGTAGACTCCGT- CTT GCACCGCGGCCCTGATAATATCCACCACCGTAGAATAAGCAACACCCAGCCAAGCAATACACTCGCTCTGCGAG- CGG CAGACAGGAGGAGCGGGCAGAGATGGGAGAACCATGATAAAAAACTTTTTTTAAAGAATATTTTCCAATTCTTC- GAA AGTAAGATCTATCAAGTGGCAGCGCTCCCCTCCACTGGCGCGGTCAAACTCTACGGCCAAAGCACAGACAACGG- CAT TTCTAAGATGTTCCTTAATGGCGTCCAAAAGACACACCGCTCTCAAGTTGCAGTAAACTATGAATGAAAACCCA- TCC GGCTGATTTTCCAATATAGACGCGCCGGCAGCGTCCACCAAACCCAGATAATTTTCTTCTCTCCAGCGGTTTAC- GAT CTGTCTAAGCAAATCCCTTATATCAAGTCCGACCATGCCAAAAATCTGCTCAAGAGCGCCCTCCACCTTCATGT- ACA AGCAGCGCATCATGATTGCAAAAATTCAGGTTCTTCAGAGACCTGTATAAGATTCAAAATGGGAACATTAACAA- AAA TTCCTCTGTCGCGCAGATCCCTTCGCAGGGCAAGCTGAACATAATCAGACAGGTCCGAACGGACCAGTGAGGCC- AAA TCCCCACCAGGAACCAGATCCAGAGACCCTATACTGATTATGACGCGCATACTCGGGGCTATGCTGACCAGCGT- AGC GCCGATGTAGGCGTGCTGCATGGGCGGCGAGATAAAATGCAAAGTGCTGGTTAAAAAATCAGGCAAAGCCTCGC- GCA AAAAAGCTAACACATCATAATCATGCTCATGCAGGTAGTTGCAGGTAAGCTCAGGAACCAAAACGGAATAACAC- ACG ATTTTCCTCTCAAACATGACTTCGCGGATACTGCGTAAAACAAAAAATTATAAATAAAAAATTAATTAAATAAC- TTA AACATTGGAAGCCTGTCTCACAACAGGAAAAACCACTTTAATCAACATAAGACGGGCCACGGGCATGCCGGCAT- AGC CGTAAAAAAATTGGTCCCCGTGATTAACAAGTACCACAGACAGCTCCCCGGTCATGTCGGGGGTCATCATGTGA- GAC TCTGTATACACGTCTGGATTGTGAACATCAGACAAACAAAGAAATCGAGCCACGTAGCCCGGAGGTATAATCAC- CCG CAGGCGGAGGTACAGCAAAACGACCCCCATAGGAGGAATCACAAAATTAGTAGGAGAAAAAAATACATAAACAC- CAG AAAAACCCTGTTGCTGAGGCAAAATAGCGCCCTCCCGATCCAAAACAACATAAAGCGCTTCCACAGGAGCAGCC- ATA ACAAAGACCCGAGTCTTACCAGTAAAAGAAAAAAGATCTCTCAACGCAGCACCAGCACCAACACTTCGCAGTGT- AAA AGGCCAAGTGCCGAGAGAGTATATATAGGAATAAAAAGTGACGTAAACGGGCAAAGTCCAAAAAACGCCCAGAA- AAA CCGCACGCGAACCTACGCCCCGAAACGAAAGCCAAAAAACACTAGACACTCCCTTCCGGCGTCAACTTCCGCTT- TCC CACGCTACGTCACTTCCCCCGGTCAAACAAACTACATATCCCGAACTTCCAAGTCGCCACGCCCAAAACACCGC- CTA CACCTCCCCGCCCGCCGGCCCGCCCCCGGACCCGCCTCCCGCCCCGCGCCGCCCATCTCATTATCATATTGGCT- TCA ATCCAAAATAAGGTATATTATTGATGATG Polynucleotide sequence encoding wild type ChAd83 SEQ ID NO: 2 CATCATCAATAATATACCTCAAACTTTTGGTGCGCGTTAATATGCAAATGAGCTGTTTGAATTTGGGGAT GCGGGGCGCTGATTGGCTGCGGGAGCGGCGACCGTTAGGGGCGGGGCGGGTGACGTTTTGATGACGTGGC CGTGAGGCGGAGCCGGTTTGCAAGTTCTCGTGGGAAAAGTGACGTCAAACGAGGTGTGGTTTGAACACGG AAATACTCAATTTTCCCGCGCTCTCTGACAGGAAATGAGGTGTTTCTGGGCGGATGCAAGTGAAAACGGG CCATTTTCGCGCGAAAACTGAATGAGGAAGTGAAAATCTGAGTAATTTCGCGTTTATGGCAGGGAGGAGT ATTTGCCGAGGGCCGAGTAGACTTTGACCGATTACGTGGGGGTTTCGATTACCGTATTTTTCACCTAAAT TTCCGCGTACGGTGTCAAAGTCCGGTGTTTTTACGTAGGCGTCAGCTGATCGCCAGGGTATTTAAACCTG CGCTCACTAGTCAAGAGGCCACTCTTGAGTGCCAGCGAGTAGAGTTTTCTCCTCCGCGCCGCGAGTCAGA TCTACACTTTGAAAGATGAGGCACTTGAGAGACCTGCCCGGTAATGTTTTCCTGGCTACTGGGAACGAGA TTCTGGAATTGGTGGTGGACGCCATGATGGGTGACGACCCTCCCGAGCCCCCTACCCCATTTGAGGCGCC TTCGCTGTACGATTTGTATGATCTGGAGGTGGATGTGCCCGAGAACGACCCCAACGAGGAGGCGGTGAAT GATTTGTTTAGCGATGCCGCGCTGCTGGCTGCCGAGCAGGCTAATACGGACTTTGGCTCAGACAGCGATT CTTCTCTCCATACCCCGAGACCCGGCAGAGGTGAGAAAAAGATCCCCGAGCTTAAAGGGGAAGAGCTCGA

CCTGCGCTGCTATGAGGAATGCTTGCCTCCGAGCGATGATGAGGAGGACGAGGAGGCGATTCGAGCTGCA GCGAACCAGGGAGTGAAAGCTGCGGGCGAAAGCTTTAGCCTGGACTGTCCTACTCTGCCCGGACACGGCT GTAAGTCTTGTGAATTTCATCGCATGAATACTGGAGATAAGAATGTGATGTGTGCCCTGTGCTATATGAG AGCTTACAACCATTGTGTTTACAGTAAGTGTGATTAACTTTAGTTGGGAAGGCAGAGGGTGACTGGGTGC TGACTGGTTTATTTATGTATATGTTTTTTATGTGTAGGTCCCGTCTCTGACGCAGATGAGACCCCCACTT CAGAGTGCATTTCATCACCCCCAGAAATTGGCGAGGAACCGCCCGAAGATATTATTCATAGACCAGTTGC AGTGAGAGTCACCGGGCGGAGAGCAGCTGTGGAGAGTTTGGATGACTTGCTACAGGGTGGGGATGAACCT TTGGACTTGTGTACCCGGAAACGCCCCAGGCACTAAGTGCCACACATGTGTGTTTACTTAAGGTGATGTC AGTATTTATAGGGTGTGGAGTGCAATAAAATCCGTGTTGACTTTAAGTGCGTGGTTTATGACTCAGGGGT GGGGACTGTGGGTATATAAGCAGGTGCAGACCTGTGTGGTCAGTTCAGAGCAGGACTCATGGAGATCTGG ACGGTCTTGGAAGACTTTCACCAGACTAGACAGCTGCTAGAGAACTCATCGGAGGGAGTCTCTTACCTGT GGAGATTCTGCTTCGGTGGGCCTCTAGCTAAGCTAGTCTATAGGGCCAAGCAGGATTATAAGGATCAATT TGAGGATATTTTGAGAGAGTGTCCTGGTATTTTTGACTCTCTCAACTTGGGCCATCAGTCTCACTTTAAC CAGAGTATTCTGAGAGCCCTTGACTTTTCCACTCCTGGCAGAACTACCGCCGCGGTAGCCTTTTTTGCCT TTATCCTTGACAAATGGAGTCAAGAAACCCATTTCAGCAGGGATTACCGTCTGGACTGCTTAGCAGTAGC TTTGTGGAGAACATGGAGGTGCCAGCGCCTGAATGCAATCTCCGGCTACTTGCCAGTACAGCCGGTAGAC ACGCTGAGGATCCTGAGTCTCCAGTCACCCCAGGAACACCAACGCCGCCAGCAGCCGCAGCAGGAGCAGC AGCAAGAGGAGGACCGAGAAGAGAACCCGAGAGCCGGTCTGGACCCTCCGGTGGCGGAGGAGGAGGAGTA GCTGACTTGTTTCCCGAGCTGCGCCGGGTGCTGACTAGGTCTTCCAGTGGACGGGAGAGGGGGATTAAGC GGGAGAGGCATGAGGAGACTAGTCACAGAACTGAACTGACTGTCAGTCTGATGAGCCGCAGGCGCCCAGA ATCGGTGTGGTGGCATGAGGTGCAGTCGCAGGGGATAGATGAGGTCTCGGTGATGCATGAGAAATATTCC CTAGAACAAGTCAAGACTTGTTGGTTGGAGCCTGAGGATGATTGGGAGGTAGCCATCAGGAATTATGCCA AGCTAGCTCTGAAGCCAGACAAGAAGTACAAGATTACCAAACTGATTAATATCAGAAATTCCTGCTACAT TTCAGGGAATGGGGCCGAGGTGGAGATCAGTACCCAGGAGAGGGTGGCCTTCAGATGCTGCATGATGAAT ATGTACCCGGGGGTGGTGGGCATGGAGGGAGTCACCTTTATGAACGCGAGGTTCAGGGGCGATGGGTATA ATGGGGTGGTCTTTATGGCCAACACCAAGCTGACAGTGCACGGATGCTCCTTCTTTGGCTTCAATAACAT GTGCATCGAGGCCTGGGGCAGTGTTTCAGTGAGGGGATGCAGTTTTTCAGCCAACTGGATGGGGGTCGTG GGCAGAACCAAGAGCAAGGTGTCAGTGAAGAAATGCCTGTTCGAGAGGTGCCACCTGGGGGTGATGAGCG AGGGCGAAGCCAAAGTCAAACACTGCGCCTCTACTGAGACGGGCTGCTTTGTGCTGATCAAGGGCAATGC CCAAGTCAAGCATAACATGATCTGTGGGGCCTCGGATGAGCGCGGCTACCAGATGCTGACCTGCGCCGGT GGGAACAGCCATATGCTGGCCACCGTGCATGTGACCTCGCACCCCCGCAAGACATGGCCCGAGTTCGAGC ACAACGTCATGACCCGCTGCAATGTGCACCTGGGCTCCCGCCGAGGCATGTTCATGCCCTACCAGTGCAA CATGCAATTTGTGAAGGTGCTGCTGGAGCCCGATGCCATGTCCAGAGTGAGCCTGACGGGGGTGTTTGAC ATGAATGTGGAGATGTGGAAAATTCTGAGATATGATGAATCCAAGACCAGGTGCCGGGCCTGCGAATGCG GAGGCAAGCACGCCAGGCTTCAGCCCGTGTGTGTGGAGGTGACGGAGGACCTGCGACCCGATCATTTGGT GTTGTCCTGCAACGGGACGGAGTTCGGCTCCAGCGGGGAAGAATCTGACTAGAGTGAGTAGTGTTTGGGG GAGGTGGAGGGCCTGGATGAGGGGCAGAATGACTAAAATCTGTGTTTTTCTGCGCAGCAGCATGAGCGGA AGCGCCTCCTTTGAGGGAGGGGTATTCAGCCCTTATCTGACGGGGCGTCTCCCCTCCTGGGCGGGAGTGC GTCAGAATGTGATGGGATCCACGGTGGACGGCCGGCCCGTGCAGCCCGCGAACTCTTCAACCCTGACCTA CGCGACCCTGAGCTCCTCGTCCGTGGACGCAGCTGCCGCCGCAGCTGCTGCTTCCGCCGCCAGCGCCGTG CGCGGAATGGCCCTGGGCGCCGGCTACTACAGCTCTCTGGTGGCCAACTCGAGTTCCACCAATAATCCCG CCAGCCTGAACGAGGAGAAGCTGCTGCTGCTGATGGCCCAGCTCGAGGCCCTGACCCAGCGCCTGGGCGA GCTGACCCAGCAGGTTGCTCAGCTGCAGGCGGAGACGCGGGCCGCGGTTGCCACGGTGAAAACCAAATAA AAAATGAATCAATAAATAAACGGAGACGGTTGTTGATTTTAACACAGAGTCTTGAATCTTTATTTGATTT TTCGCGCGCGGTAGGCCCTGGACCACCGGTCTCGATCATTGAGCACCCGGTGGATCTTTTCCAGGACCCG GTAGAGGTGGGCTTGGATGTTGAGGTACATGGGCATGAGCCCGTCCCGGGGGTGGAGGTAGCTCCATTGC AGGGCCTCGTGCTCGGGGGTGGTGTTGTAAATCACCCAGTCATAGCAGGGGCGCAGGGCGTGGTGCTGCA CGATGTCCTTGAGGAGGAGACTGATGGCCACGGGCAGCCCCTTGGTGTAGGTGTTGACGAACCTGTTGAG CTGGGAGGGATGCATGCGGGGGGAGATGAGATGCATCTTGGCCTGGATCTTGAGATTGGCGATGTTCCCG CCCAGATCCCGCCGGGGGTTCATGTTGTGCAGGACCACCAGCACGGTGTATCCGGTGCACTTGGGGAATT TGTCATGCAACTTGGAAGGGAAGGCGTGAAAGAATTTGGAGACGCCCTTGTGGCCGCCCAGGTTTTCCAT GCACTCATCCATGATGATGGCGATGGGCCCGTGGGCGGCGGCCTGGGCAAAGACGTTTCGGGGGTCGGAC ACATCGTAGTTGTGGTCCTGGGTGAGCTCGTCATAGGCCATTTTAATGAATTTGGGGCGGAGGGTGCCCG ACTGGGGGACGAAGGTGCCCTCGATCCCGGGGGCGTAGTTGCCCTCGCAGATCTGCATCTCCCAGGCCTT GAGCTCGGAGGGGGGGATCATGTCCACCTGCGGGGCGATGAAAAAAACGGTTTCCGGGGCGGGGGAGATG AGCTGCGCCGAAAGCAGGTTCCGGAGCAGCTGGGACTTGCCGCAGCCGGTGGGGCCGTAGATGACCCCGA TGACCGGCTGCAGGTGGTAGTTGAGGGAGAGACAGCTGCCGTCCTCGCGGAGGAGGGGGGCCACCTCGTT CATCATCTCGCGCACATGCATGTTCTCGCGCACGAGTTCCGCCAGGAGGCGCTCGCCCCCCAGCGAGAGG AGCTCTTGCAGCGAGGCGAAGTTTTTCAGCGGCTTGAGCCCGTCGGCCATGGGCATTTTGGAGAGGGTCT GTTGCAAGAGTTCCAGACGGTCCCAGAGCTCGGTGATGTGCTCTAGGGCATCTCGATCCAGCAGACCTCC TCGTTTCGCGGGTTGGGGCGACTGCGGGAGTAGGGCACCAGGCGATGGGCGTCCAGCGAGGCCAGGGTCC GGTCCTTCCAGGGTCGCAGGGTCCGCGTCAGCGTGGTCTCCGTCACGGTGAAGGGGTGCGCGCCGGGCTG GGCGCTTGCGAGGGTGCGCTTCAGGCTCATCCGGCTGGTCGAGAACCGCTCCCGGTCGGCGCCCTGTGCG TCGGCCAGGTAGCAATTGAGCATGAGTTCGTAGTTGAGCGCCTCGGCCGCGTGGCCCTTGGCGCGGAGCT TACCTTTGGAAGTGTGTCCGCAGACGGGACAGAGGAGGGACTTGAGGGCGTAGAGCTTGGGGGCGAGGAA GACGGACTCGGGGGCGTAGGCGTCCGCGCCGCAGCTGGCGCAGACGGTCTCGCACTCCACGAGCCAGGTG AGGTCGGGGCGGTCGGGGTCAAAAACGAGGTTTCCTCCGTGCTTTTTGATGCGTTTCTTACCTCTGGTCT CCATGAGCTCGTGTCCCCGCTGGGTGACAAAGAGGCTGTCCGTGTCCCCGTAGACCGACTTTATGGGCCG GTCCTCGAGCGGGGTGCCGCGGTCCTCGTCGTAGAGGAACCCCGCCCACTCCGAGACGAAGGCCCGGGTC CAGGCCAGCACGAAGGAGGCCACGTGGGAGGGGTAGCGGTCGTTGTCCACCAGCGGGTCCACCTTCTCCA GGGTATGCAAGCACATGTCCCCCTCGTCCACATCCAGGAAGGTGATTGGCTTGTAAGTGTAGGCCACGTG ACCGGGGGTCCCGGCCGGGGGGGTATAAAAGGGGGCGGGCCCCTGCTCGTCCTCACTGTCTTCCGGATCG CTGTCCAGGAGCGCCAGCTGTTGGGGTAGGTATTCCCTCTCGAAGGCGGGCATGACCTCGGCACTCAGGT TGTCAGTTTCTAGAAACGAGGAGGATTTGATATTGACGGTGCCGTTGGAGACGCCTTTCATGAGCCCCTC GTCCATCTGGTCAGAAAAGACGATCTTTTTGTTGTCGAGCTTGGTGGCGAAGGAGCCGTAGAGGGCGTTG GAGAGCAGCTTGGCGATGGAGCGCATGGTCTGGTTCTTTTCCTTGTCGGCGCGCTCCTTGGCGGCGATGT TGAGCTGCACGTACTCGCGCGCCACGCACTTCCATTCGGGGAAGACGGTGGTGAGCTCGTCGGGCACGAT TCTGACCCGCCAGCCGCGGTTGTGCAGGGTGATGAGGTCCACGCTGGTGGCCACCTCGCCGCGCAGGGGC TCGTTGGTCCAGCAGAGGCGCCCGCCCTTGCGCGAGCAGAAGGGGGGCAGCGGGTCCAGCATGAGCTCGT CGGGGGGGTCGGCGTCCACGGTGAAGATGCCGGGCAGGAGCTCGGGGTCGAAGTAGCTGATGCAGGTGCC CAGATCGTCCAGCGCCGCTTGCCAGTCGCGCACGGCCAGCGCGCGCTCGTAGGGGCTGAGGGGCGTGCCC CAGGGCATGGGGTGCGTGAGCGCGGAGGCGTACATGCCGCAGATGTCGTAGACGTAGAGGGGCTCCTCGA GGACGCCGATGTAGGTGGGGTAGCAGCGCCCCCCGCGGATGCTGGCGCGCACGTAGTCGTACAGCTCGTG CGAGGGCGCGAGGAGCCCCGTGCCGAGGTTGGAGCGTTGCGGCTTTTCGGCGCGGTAGACGATCTGGCGG AAGATGGCGTGGGAGTTGGAGGAGATGGTGGGCCTCTGGAAGATGTTGAAGTGGGCGTGGGGCAGGCCGA CCGAGTCCCTGATGAAGTGGGCGTAGGAGTCCTGCAGCTTGGCGACGAGCTCGGCGGTGACGAGGACGTC CAGGGCGCAGTAGTCGAGGGTCTCTTGGATGATGTCGTACTTGAGCTGGCCCTTCTGCTTCCACAGCTCG CGGTTGAGAAGGAACTCTTCGCGGTCCTTCCAGTACTCTTCGAGGGGGAACCCGTCCTGATCGGCACGGT AAGAGCCCACCATGTAGAACTGGTTGACGGCCTTGTAGGCGCAGCAGCCCTTCTCCACGGGGAGGGCGTA AGCTTGCGCGGCCTTGCGCAGGGAGGTGTGGGTGAGGGCGAAGGTGTCGCGCACCATGACTTTGAGGAAC TGGTGCTTGAAGTCGAGGTCGTCGCAGCCGCCCTGCTCCCAGAGTTGGAAGTCCGTGCGCTTCTTGTAGG CGGGGTTGGGCAAAGCGAAAGTAACATCGTTGAAGAGGATCTTGCCCGCGCGGGGCATGAAGTTGCGAGT GATGCGGAAAGGCTGGGGCACCTCGGCCCGGTTGTTGATGACCTGGGCGGCGAGGACGATCTCGTCGAAG CCGTTGATGTTGTGCCCGACGATGTAGAGTTCCACGAATCGCGGGCAGCCCTTGACGTGGGGCAGCTTCT TGAGCTCGTCGTAGGTGAGCTCGGCGGGGTCGCTGAGCCCGTGCTGCTCGAGGGCCCAGTCGGCGACGTG GGGGTTGGCGCTGAGGAAGGAAGTCCAGAGATCCACGGCCAGGGCGGTCTGCAAGCGGTCCCGGTACTGA CGGAACTGCTGGCCCACGGCCATTTTTTCGGGGGTGACGCAGTAGAAGGTGCGGGGGTCGCCGTGCCAGC GGTCCCACTTGAGTTGGAGGGCGAGGTCGTGGGCGAGCTCGACGAGCGGCGGGTCCCCGGAGAGTTTCAT GACCAGCATGAAGGGGACGAGCTGCTTGCCGAAGGACCCCATCCAGGTGTAGGTTTCCACATCGTAGGTG AGGAAGAGCCTTTCGGTGCGAGGATGCGAGCCGATGGGGAAGAACTGGATCTCCTGCCACCAGTTGGAGG AATGGCTGTTGATGTGATGGAAGTAGAAATGCCGACGGCGCGCCGAGCACTCGTGCTTGTGTTTATACAA GCGTCCGCAGTGCTCGCAACGCTGCACGGGATGCACGTGCTGCACGAGCTGTACCTGGGTTCCTTTGACG AGGAATTTCAGTGGGCAGTGGAGCGCTGGCGGCTGCATCTGGTGCTGTACTACGTCCTGGCCATCGGCGT GGCCATCGTCTGCCTCGATGGTGGTCATGCTGACGAGCCCGCGCGGGAGGCAGGTCCAGACCTCGGCTCG GACGGGTCGGAGAGCGAGGACGAGGGCGCGCAGGCCGGAGCTGTCCAGGGTCCTGAGACGCTGCGGAGTC AGGTCAGTGGGCAGCGGCGGCGCGCGGTTGACTTGCAGGAGCTTTTCCAGGGCGCGCGGGAGGTCCAGAT GGTACTTGATCTCCACGGCGCCGTTGGTGGCGACGTCCACGGCTTGCAGGGTCCCGTGCCCCTGGGGCGC CACCACCGTGCCCCGTTTCTTCTTGGGCGGCGGCGGCTCCATGCTTAGAAGCGGCGGCGAGGACGCGCGC CGGGCGGCAGGGGCGGCTCGGGGCCCGGAGGCAGGGGCGGCAGGGGCACGTCGGCGCCGCGCGCGGGCAG GTTCTGGTACTGCGCCCGGAGAAGACTGGCGTGAGCGACGACGCGACGGTTGACGTCCTGGATCTGACGC CTCTGGGTGAAGGCCACGGGACCCGTGAGTTTGAACCTGAAAGAGAGTTCGACAGAATCAATTTCGGTAT CGTTGACGGCGGCCTGCCGCAGGATCTCTTGCACGTCGCCCGAGTTGTCCTGGTAGGCGATCTCGGTCAT GAACTGCTCGATCTCCTCCTCCTGAAGGTCTCCGCGGCCGGCGCGCTCGACGGTGGCCGCGAGGTCGTTG GAGATGCGGCCCATGAGCTGCGAGAAGGCGTTCATGCCGGCCTCGTTCCAGACGCGGCTGTAGACCACGG CTCCGTTGGGGTCGCGCGCGCGCATGACCACCTGGGCGAGGTTAAGCTCGACGTGGCGCGTGAAGACCGC GTAGTTGCAGAGGCGCTGGTAGAGGTAGTTGAGCGTGGTGGCGATGTGCTCGGTGACGAAGAAGTACATG ATCCAGCGGCGGAGCGGCATCTCGCTGACGTCGCCCAGGGCTTCCAAGCGCTCCATGGTCTCGTAGAAGT CCACGGCGAAGTTGAAAAACTGGGAGTTGCGCGCCGAGACGGTCAACTCCTCCTCCAGAAGACGGATGAG CTCGGCGATGGTGGCGCGCACCTCGCGCTCGAAGGCCCCGGGGGGCTCCTCTTCTTCCATCTCCTCCTCC TCTTCCTCCTCCACTAACATCTCTTCTACTTCCTCCTCAGGAGGCGGCGGCGGGGGAGGGGCCCTGCGTC GCCGGCGGCGCACGGGCAGACGGTCGATGAAGCGCTCGATGGTCTCCCCGCGCCGGCGACGCATGGTCTC GGTGACGGCGCGCCCGTCCTCGCGGGGCCGCAGCGTGAAGACGCCGCCGCGCATCTCCAGGTGGCCGCCG GGGGGGTCTCCGTTGGGCAGGGAGAGGGCGCTGACGATGCATCTTATCAATTGGCCCGTAGGGACTCCGC GCAAGGACCTGAGCGTCTCGAGATCCACGGGATCCGAAAACCGCTGAACGAAGGCTTCGAGCCAGTCGCA GTCGCAAGGTAGGCTGAGCCCGGTTTCTTGTTCTTCGGGTATTTGGTCGGGAGGCGGGCGGGCGATGCTG CTGGTGATGAAGTTGAAGTAGGCGGTCCTGAGACGGCGGATGGTGGCGAGGAGCACCAGGTCCTTGGGCC

CGGCTTGCTGGATGCGCAGACGGTCGGCCATGCCCCAGGCGTGGTCCTGACACCTGGCGAGGTCCTTGTA GTAGTCCTGCATGAGCCGCTCTACGGGCACGTCCTCCTCGCCCGCGCGGCCGTGCATGCGCGTGAGCCCG AACCCGCGCTGCGGCTGGACGAGCGCCAGGTCGGCGACGACGCGCTCGGCGAGGATGGCCTGCTGGATCT GGGTGAGGGTGGTCTGGAAGTCGTCGAAGTCGACGAAGCGGTGGTAGGCTCCGGTGTTGATGGTGTAGGA GCAGTTGGCCATGACGGACCAGTTGACGGTCTGGTGGCCGGGGCGCACGAGCTCGTGGTACTTGAGGCGC GAGTAGGCGCGCGTGTCGAAGATGTAGTCGTTGCAGGTGCGCACGAGGTACTGGTATCCGACGAGGAAGT GCGGCGGCGGCTGGCGGTAGAGCGGCCATCGCTCGGTGGCGGGGGCGCCGGGCGCGAGGTCCTCGAGCAT GAGGCGGTGGTAGCCGTAGATGTACCTGGACATCCAGGTGATGCCGGCGGCGGTGGTGGAGGCGCGCGGG AACTCGCGGACGCGGTTCCAGATGTTGCGCAGCGGCAGGAAGTAGTTCATGGTGGCCGCGGTCTGGCCCG TGAGGCGCGCGCAGTCGTGGATGCTCTAGACATACGGGCAAAAACGAAAGCGGTCAGCGGCTCGACTCCG TGGCCTGGAGGCTAAGCGAACGGGTTGGGCTGCGCGTGTACCCCGGTTCGAATCTCGAATCAGGCTGGAG CCGCAGCTAACGTGGTACTGGCACTCCCGTCTCGACCCAAGCCTGCTAACGAAACCTCCAGGATACGGAG GCGGGTCGTTTTTTGGCCTTGGTCGCTGGTCATGAAAAACTAGTAAGCGCGGAAAGCGGCCGCCCGCGAT GGCTCGCTGCCGTAGTCTGGAGAAAGAATCGCCAGGGTTGCGTTGCGGTGTGCCCCGGTTCGAGCCTCAG CGCTCGGTGCCGGCCGGATTCCGCGGCTAACGTGGGCGTGGCTGCCCCGTCGTTTCCAAGACCCCTTAGC CAGCCGACTTCTCCAGTTACGGAGCGAGCCCCTCTTTTTCTTGTGTTTTTGCCAGATGCATCCCGTACTG CGGCAGATGCGCCCCCACCCTCCACCACAACCGCCCCTACCGCAGCAGCAGCAACAGCCGGCGCTTCTGC CCCCGCCCCAGCAGCAGCAGCCAGCCACTACCGCGGCGGCCGCCGTGAGCGGAGCCGGCGTTCAGTATGA CCTGGCCTTGGAAGAGGGCGAGGGGCTGGCGCGGCTGGGGGCGTCGTCGCCGGAGCGGCACCCGCGCGTG CAGATGAAAAGGGACGCTCGCGAGGCCTACGTGCCCAAGCAGAACCTGTTCAGAGACAGGAGCGGCGAGG AGCCCGAGGAGATGCGCGCCTCCCGCTTCCACGCGGGGCGGGAGCTGCGGCGCGGCCTGGACCGAAAGCG GGTGCTGAGGGACGAGGATTTCGAGGCGGACGAGCTGACGGGGATCAGCCCCGCGCGCGCGCACGTGGCC GCGGCCAACCTGGTCACGGCGTACGAGCAGACCGTGAAGGAGGAGAGCAACTTTCAAAAATCCTTCAACA ACCACGTGCGCACGCTGATCGCGCGCGAGGAGGTGACCCTGGGCCTGATGCACCTGTGGGACCTGCTGGA GGCCATCGTGCAGAACCCCACGAGCAAGCCGCTGACGGCGCAGCTGTTTCTGGTGGTGCAGCACAGTCGG GACAACGAGACGTTCAGGGAGGCGCTGCTGAATATCACCGAGCCCGAGGGCCGCTGGCTCCTGGACCTGG TGAACATTCTGCAGAGCATCGTGGTGCAGGAGCGCGGGCTGCCGCTGTCCGAGAAGCTGGCGGCCATCAA CTTCTCGGTGCTGAGCCTGGGCAAGTACTACGCTAGGAAGATCTACAAGACCCCGTACGTGCCCATAGAC AAGGAGGTGAAGATCGACGGGTTTTACATGCGCATGACCCTGAAAGTGCTGACCCTGAGCGACGATCTGG GGGTGTACCGCAACGACAGGATGCACCGCGCGGTGAGCGCCAGCCGCCGGCGCGAGCTGAGCGACCAGGA GCTGATGCACAGCCTGCAGCGGGCCCTGACCGGGGCCGGGACCGAGGGGGAGAGCTACTTTGACATGGGC GCGGACCTGCGCTGGCAGCCCAGCCGCCGGGCCTTGGAAGCTGCCGGCGGCGTGCCCTACGTGGAGGAGG TGGACGATGAGGAGGAGGAGGGCGAGTACCTGGAAGACTGATGGCGCGACCGTATTTTTGCTAGATGCAG CAACAGCCACCGCCGCCTCCTGATCCCGCGATGCGGGCGGCGCTGCAGAGCCAGCCGTCCGGCATTAACT CCTCGGACGATTGGACCCAGGCCATGCAACGCATCATGGCGCTGACGACCCGCAATCCCGAAGCCTTTAG ACAGCAGCCTCAGGCCAACCGGCTCTCGGCCATCCTGGAGGCCGTGGTGCCCTCGCGCTCGAACCCCACG CACGAGAAGGTGCTGGCCATCGTGAACGCGCTGGTGGAGAACAAGGCCATCCGCGGCGACGAGGCCGGGC TGGTGTACAACGCGCTGCTGGAGCGCGTGGCCCGCTACAACAGCACCAACGTGCAGACGAACCTGGACCG CATGGTGACCGACGTGCGCGAGGCGGTGTCGCAGCGCGAGCGGTTCCACCGCGAGTCGAACCTGGGCTCC ATGGTGGCGCTGAACGCCTTCCTGAGCACGCAGCCCGCCAACGTGCCCCGGGGCCAGGAGGACTACACCA ACTTCATCAGCGCGCTGCGGCTGATGGTGGCCGAGGTGCCCCAGAGCGAGGTGTACCAGTCGGGGCCGGA CTACTTCTTCCAGACCAGTCGCCAGGGCTTGCAGACCGTGAACCTGAGCCAGGCTTTCAAGAACTTGCAG GGACTGTGGGGCGTGCAGGCCCCGGTCGGGGACCGCGCGACGGTGTCGAGCCTGCTGACGCCGAACTCGC GCCTGCTGCTGCTGCTGGTGGCGCCCTTCACGGACAGCGGCAGCGTGAGCCGCGACTCGTACCTGGGCTA CCTGCTTAACCTGTACCGCGAGGCCATCGGGCAGGCGCACGTGGACGAGCAGACCTACCAGGAGATCACC CACGTGAGCCGCGCGCTGGGCCAGGAGGACCCGGGCAACCTGGAGGCCACCCTGAACTTCCTGCTGACCA ACCGGTCGCAGAAGATCCCGCCCCAGTACGCGCTGAGCACCGAGGAGGAGCGCATCCTGCGCTACGTGCA GCAGAGCGTGGGGCTGTTCCTGATGCAGGAGGGGGCCACGCCCAGCGCCGCGCTCGACATGACCGCGCGC AACATGGAGCCCAGCATGTACGCCCGCAACCGCCCGTTCATCAATAAGCTGATGGACTACTTGCATCGGG CGGCCGCCATGAACTCGGACTACTTTACCAACGCCATCTTGAACCCGCACTGGCTCCCGCCGCCCGGGTT CTACACGGGCGAGTACGACATGCCCGACCCCAACGACGGGTTCCTGTGGGATGACGTGGACAGCAGCGTG TTCTCGCCGCGTCCCACCACCACCGTGTGGAAGAAAGAGGGCGGGGACCGGCGGCCGTCCTCGGCGCTGT CCGGTCGCGCGGGTGCTGCCGCGGCGGTGCCCGAGGCCGCCAGCCCCTTTCCGAGCCTGCCCTTTTCGCT GAACAGCGTGCGCAGCAGCGAGCTGGGTCGGCTGACGCGGCCGCGCCTGCTGGGCGAGGAGGAGTACCTG AACGACTCCTTGTTGAGGCCCGAGCGCGAAAAGAACTTCCCCAATAACGGGATAGAGAGCCTGGTGGACA AGATGAGCCGCTGGAAGACGTACGCGCACGAGCACAGGGACGAGCCCCGAGCTAGCAGCGCAGGCACCCG TAGACGCCAGCGGCACGACAGGCAGCGGGGTCTGGTGTGGGACGATGAGGATTCCGCCGACGACAGCAGC GTGTTGGACTTGGGTGGGAGTGGTGGTGGTAACCCGTTCGCTCACTTGCGCCCCCGTATCGGGCGCCTGA TGTAAGAATCTGAAAAATAAAAAACGGTACTCACCAAGGCCATGGCGACCAGCGTGCGTTCTTCTCTGTT GTTTGTAGTAGTATGATGAGGCGCGTGTACCCGGAGGGTCCTCCTCCCTCGTACGAGAGCGTGATGCAGC AGGCGGTGGCGGCGGCGATGCAGCCCCCGCTGGAGGCGCCTTACGTGCCCCCGCGGTACCTGGCGCCTAC GGAGGGGCGGAACAGCATTCGTTACTCGGAGCTGGCACCCTTGTACGATACCACCCGGTTGTACCTGGTG GACAACAAGTCGGCGGACATCGCCTCGCTGAACTACCAGAACGACCACAGCAACTTCCTGACCACCGTGG TGCAGAACAACGATTTCACCCCCACGGAGGCCAGCACCCAGACCATCAACTTTGACGAGCGCTCGCGGTG GGGCGGCCAGCTGAAAACCATCATGCACACCAACATGCCCAACGTGAACGAGTTCATGTACAGCAACAAG TTCAAGGCGCGGGTGATGGTCTCGCGCAAGACCCCCAACGGGGTCACAGTAACAGATGGTAGTCAGGACG AGCTGACCTACGAGTGGGTGGAGTTTGAGCTGCCCGAGGGCAACTTCTCGGTGACCATGACCATCGATCT GATGAACAACGCCATCATCGACAACTACTTGGCGGTGGGGCGGCAGAACGGGGTGCTGGAGAGCGACATC GGCGTGAAGTTCGACACGCGCAACTTCCGGCTGGGCTGGGACCCCGTGACCGAGCTGGTGATGCCGGGCG TGTACACCAACGAGGCCTTCCACCCCGACATCGTCCTGCTGCCCGGCTGCGGCGTGGACTTCACCGAGAG CCGCCTCAGCAACCTGCTGGGCATCCGCAAGCGGCAGCCCTTCCAGGAGGGCTTCCAGATCCTGTACGAG GACCTGGAGGGGGGCAACATCCCCGCGCTCTTGGATGTCGAAGCCTACGAGAAAAGCAAGGAGGATAGCA CCGCCGTGGCTACCGCCGCGACTGTGGCAGATGCCACTGTCACCAGGGGCGATACATTCGCCACCCAGGC GGAGGAAGCAGCCGCCCTAGCGGCGACCGATGATAGTGAAAGTAAGATAGTTATCAAGCCGGTGGAGAAG GACAGCAAGGACAGGAGCTACAACGTTCTATCGGATGGAAAGAACACCGCCTACCGCAGCTGGTACCTGG CCTACAACTACGGCGACCCCGAGAAGGGCGTGCGCTCCTGGACGCTGCTCACCACCTCGGACGTCACCTG CGGCGTGGAGCAAGTCTACTGGTCGCTGCCCGACATGATGCAAGACCCGGTCACCTTCCGCTCCACGCGT CAAGTTAGCAACTACCCGGTGGTGGGCGCCGAGCTCCTGCCCGTCTACTCCAAGAGCTTCTTCAACGAGC AGGCCGTCTACTCGCAGCAGCTGCGCGCCTTCACCTCGCTCACGCACGTCTTCAACCGCTTCCCCGAGAA CCAGATCCTCGTCCGCCCGCCCGCGCCCACCATTACCACCGTCAGTGAAAACGTTCCTGCTCTCACAGAT CACGGGACCCTGCCGCTGCGCAGCAGTATCCGGGGAGTCCAGCGCGTGACCGTCACTGACGCCAGACGCC GCACCTGCCCCTACGTCTACAAGGCCCTGGGCGTAGTCGCGCCGCGCGTCCTCTCGAGCCGCACCTTCTA AAAAATGTCCATTCTCATCTCGCCCAGTAATAACACCGGTTGGGGCCTGCGCGCGCCCAGCAAGATGTAC GGAGGCGCTCGCCAACGCTCCACGCAACACCCCGTGCGCGTGCGCGGGCACTTCCGCGCTCCCTGGGGCG CCCTCAAGGGTCGCGTGCGCTCGCGCACCACCGTCGACGACGTGATCGACCAGGTGGTGGCCGACGCGCG CAACTACACGCCCGCCGCCGCGCCCGCCTCCACCGTGGACGCCGTCATCGACAGCGTGGTGGCCGACGCG CGCCGGTACGCCCGCGCCAAGAGCCGGCGGCGGCGCATCGCCCGGCGGCACCGGAGCACCCCCGCCATGC GCGCGGCGCGAGCCTTGCTGCGCAGGGCCAGGCGCACGGGACGCAGGGCCATGCTCAGGGCGGCCAGACG CGCGGCCTCCGGCAGCAGCAGCGCCGGCAGGACCCGCAGACGCGCGGCCACGGCGGCGGCGGCGGCCATC GCCAGCATGTCCCGCCCGCGGCGCGGCAACGTGTACTGGGTGCGCGACGCCGCCACCGGTGTGCGCGTGC CCGTGCGCACCCGCCCCCCTCGCACTTGAAGATGCTGACTTCGCGATGTTGATGTGTCCCAGCGGCGAGG AGGATGTCCAAGCGCAAATACAAGGAAGAGATGCTCCAGGTCATCGCGCCTGAGATCTACGGCCCCGCGG CGGCGGTGAAGGAGGAAAGAAAGCCCCGCAAACTGAAGCGGGTCAAAAAGGACAAAAAGGAGGAGGAAGA TGTGGACGGACTGGTGGAGTTTGTGCGCGAGTTCGCCCCCCGGCGGCGCGTGCAGTGGCGCGGGCGGAAA GTGAAACCGGTGCTGCGGCCCGGCACCACGGTGGTCTTCACGCCCGGCGAGCGTTCCGGCTCCGCCTCCA AGCGCTCCTACGACGAGGTGTACGGGGACGAGGACATCCTCGAGCAGGCGGCCGAGCGTCTGGGCGAGTT TGCTTACGGCAAGCGCAGCCGCCCCGCGCCCTTGAAAGAGGAGGCGGTGTCCATCCCGCTGGACCACGGC AACCCCACGCCGAGCCTGAAGCCGGTGACCCTGCAGCAGGTGCTGCCGAGCGCGGCGCCGCGCCGGGGCT TCAAGCGCGAGGGCGGCGAGGATCTGTACCCGACCATGCAGCTGATGGTGCCCAAGCGCCAGAAGCTGGA GGACGTGCTGGAGCACATGAAGGTGGACCCCGAGGTGCAGCCCGAGGTCAAGGTGCGGCCCATCAAGCAG GTGGCCCCGGGCCTGGGCGTGCAGACCGTGGACATCAAGATCCCCACGGAGCCCATGGAAACGCAGACCG AGCCCGTGAAGCCCAGCACCAGCACCATGGAGGTGCAGACGGATCCCTGGATGCCGGCGCCGGCTTCCAC CACCACTCGCCGAAGACGCAAGTACGGCGCGGCCAGCCTGCTGATGCCCAACTACGCGCTGCATCCTTCC ATCATCCCCACGCCGGGCTACCGCGGCACGCGCTTCTACCGCGGCTACAGCAGCCGCCGCAAGACCACCA CCCGCCGCCGCCGTCGCCGCACCCGCCGCAGCACCACCGCGACTTCCGCCGCCGCCTTGGTGCGGAGAGT GTACCGCAGCGGGCGTGAGCCTCTGACCCTGCCGCGCGCGCGCTACCACCCGAGCATCGCCATTTAACTC TGCCGTCGCCTCCTTGCAGATATGGCCCTCACATGCCGCCTCCGCGTCCCCATTACGGGCTACCGAGGAA GAAAGCCGCGCCGTAGAAGGCTGACGGGGAACGGGCTGCGTCGCCATCACCACCGGCGGCGGCGCGCCAT CAGCAAGCGGTTGGGGGGAGGCTTCCTGCCCGCGCTGATCCCCATCATCGCCGCGGCGATCGGGGCGATC CCCGGCATAGCTTCCGTGGCGGTGCAGGCCTCTCAGCGCCACTGAGACACAGCTTGGAAAATTTGTAATA AAAAAATGGACTGACGCTCCTGGTCCTGTGATGTGTGTTTTTAGATGGAAGACATCAATTTTTCGTCCCT GGCACCGCGACACGGCACGCGGCCGTTTATGGGCACCTGGAGCGACATCGGCAACAGCCAACTGAACGGG GGCGCCTTCAATTGGAGCAGTCTCTGGAGCGGGCTTAAGAATTTCGGGTCCACGCTCAAAACCTATGGCA ACAAGGCGTGGAACAGCAGCACAGGGCAGGCGCTGAGGGAAAAGCTGAAAGAGCAGAACTTCCAGCAGAA GGTGGTCGATGGCCTGGCCTCGGGCATCAACGGGGTGGTGGACCTGGCCAACCAGGCCGTGCAGAAACAG ATCAACAGCCGCCTGGACGCGGTCCCGCCCGCGGGGTCCGTGGAGATGCCCCAGGTGGAGGAGGAGCTGC CTCCCCTGGACAAGCGCGGCGACAAGCGACCGCGTCCCGACGCGGAGGAGACGCTGCTGACGCACACGGA CGAGCCGCCCCCGTACGAGGAGGCGGTGAAACTGGGTCTGCCCACCACGCGGCCCGTGGCGCCTCTGGCC ACCGGGGTGCTGAAACCCAGCAGCAGCAGCAGCCAGCCCGCGACCCTGGACTTGCCTCCACCTCGCCCCT CCACAGTGGCTAAGCCCCTGCCGCCGGTGGCCGTCGCGTCGCGCGCCCCCCGAGGCCGCCCCCAGGCGAA CTGGCAGAGCACTCTGAACAGCATCGTGGGTCTGGGAGTGCAGAGTGTGAAGCGCCGCCGCTGCTATTAA AAGACACTGTAGCGCTTAACTTGCTTGTCTGTGTGTATATGTATGTCCGCCGACCAGAAGGAGGAGGAAG AGGCGCGTCGCCGAGTTGCAAGATGGCCACCCCATCGATGCTGCCCCAGTGGGCGTACATGCACATCGCC GGACAGGACGCTTCGGAGTACCTGAGTCCGGGTCTGGTGCAGTTCGCCCGCGCCACAGACACCTACTTCA GTCTGGGGAACAAGTTTAGGAACCCCACGGTGGCACCCACGCACGATGTGACCACCGACCGCAGCCAGCG

GCTGACGCTGCGCTTCGTGCCCGTGGACCGCGAGGACAACACCTACTCGTACAAAGTGCGCTACACGCTG GCCGTGGGCGACAACCGCGTGCTGGACATGGCCAGCACCTACTTTGACATCCGCGGCGTGCTGGATCGGG GCCCCAGCTTCAAACCCTACTCCGGCACCGCCTACAACAGCCTGGCTCCCAAGGGAGCGCCCAACACCTC ACAGTGGATAACCAAAGACAATGGAACTGATAAGACATACAGTTTTGGAAATGCTCCAGTCAGAGGATTG GACATTACAGAAGAGGGTCTCCAAATAGGAACCGATGAGTCAGGGGGTGAAAGCAAGAAAATTTTTGCAG ACAAAACCTATCAGCCTGAACCTCAGCTTGGAGATGAGGAATGGCATGATACTATTGGAGCTGAAGACAA GTATGGAGGCAGAGCGCTTAAACCTGCCACCAACATGAAACCCTGCTATGGGTCTTTCGCCAAGCCAACT AATGCTAAGGGAGGTCAGGCTAAAAGCAGAACCAAGGACGATGGCACTACTGAGCCTGATATTGACATGG CCTTCTTTGACGATCGCAGTCAGCAAGCTAGTTTCAGTCCAGAACTTGTTTTGTATACTGAGAATGTCGA TCTGGACACCCCGGATACCCACATTATTTACAAACCTGGCACTGATGAAACAAGTTCTTCTTTCAACTTG GGTCAGCAGTCCATGCCCAACAGACCCAACTACATTGGCTTCAGAGACAACTTTATCGGGCTCATGTACT ACAACAGCACTGGCAATATGGGTGTACTGGCCGGTCAGGCCTCCCAGCTGAATGCTGTGGTGGACTTGCA GGACAGAAACACTGAACTGTCCTACCAGCTCTTGCTTGACTCTCTGGGTGACAGAACCAGGTATTTCAGT ATGTGGAATCAGGCGGTGGACAGCTATGACCCCGATGTGCGCATTATTGAAAATCACGGTGTGGAGGATG AACTCCCCAACTATTGCTTCCCTTTGAATGGTGTGGGCTTTACAGATACATTCCAGGGAATTAAGGTTAA AACTACAAATAACGGAACAGCAAATGCTACAGAGTGGGAATCTGATACCTCTGTCAATAATGCTAATGAG ATTGCCAAGGGCAATCCTTTCGCCATGGAGATCAACATCCAGGCCAACCTGTGGCGGAACTTCCTCTACG CGAACGTGGCGCTGTACCTGCCCGACTCCTACAAGTACACGCCGGCCAACATCACGCTGCCCACCAACAC CAACACCTACGATTACATGAACGGCCGCGTGGTGGCGCCCTCGCTGGTGGACGCCTACATCAACATCGGG GCGCGCTGGTCGCTGGACCCCATGGACAACGTCAACCCCTTCAACCACCACCGCAACGCGGGCCTGCGCT ACCGCTCCATGCTCCTGGGCAACGGGCGCTACGTGCCCTTCCACATCCAGGTGCCCCAAAAGTTTTTCGC CATCAAGAGCCTCCTGCTCCTGCCCGGGTCCTACACCTACGAGTGGAACTTCCGCAAGGACGTCAACATG ATCCTGCAGAGCTCCCTCGGCAACGACCTGCGCACGGACGGGGCCTCCATCGCCTTCACCAGCATCAACC TCTACGCCACCTTCTTCCCCATGGCGCACAACACCGCCTCCACGCTCGAGGCCATGCTGCGCAACGACAC CAACGACCAGTCCTTCAACGACTACCTCTCGGCGGCCAACATGCTCTACCCCATCCCGGCCAACGCCACC AACGTGCCCATCTCCATCCCCTCGCGCAACTGGGCCGCCTTCCGCGGATGGTCCTTCACGCGCCTCAAGA CCCGCGAGACGCCCTCGCTCGGCTCCGGGTTCGACCCCTACTTCGTCTACTCGGGCTCCATCCCCTACCT CGACGGCACCTTCTACCTCAACCACACCTTCAAGAAGGTCTCCATCACCTTCGACTCCTCCGTCAGCTGG CCCGGCAACGACCGCCTCCTGACGCCCAACGAGTTCGAAATCAAGCGCACCGTCGACGGAGAGGGGTACA ACGTGGCCCAGTGCAACATGACCAAGGACTGGTTCCTGGTCCAGATGCTGGCCCACTACAACATCGGCTA CCAGGGCTTCTACGTGCCCGAGGGCTACAAGGACCGCATGTACTCCTTCTTCCGCAACTTCCAGCCCATG AGCCGCCAGGTCGTGGACGAGGTCAACTACAAGGACTACCAGGCCGTCACCCTGGCCTACCAGCACAACA ACTCGGGCTTCGTCGGCTACCTCGCGCCCACCATGCGCCAGGGCCAGCCCTACCCCGCCAACTACCCCTA CCCGCTCATCGGCAAGAGCGCCGTCGCCAGCGTCACCCAGAAAAAGTTCCTCTGCGACCGGGTCATGTGG CGCATCCCCTTCTCCAGCAACTTCATGTCCATGGGCGCGCTCACCGACCTCGGCCAGAACATGCTCTACG CCAACTCCGCCCACGCGCTAGACATGAATTTCGAAGTCGACCCCATGGATGAGTCCACCCTTCTCTATGT TGTCTTCGAAGTCTTCGACGTCGTCCGAGTGCACCAGCCCCACCGCGGCGTCATCGAGGCCGTCTACCTG CGCACGCCCTTCTCGGCCGGCAACGCCACCACCTAAGCCTCTTGCTTCTTGCAAGATGACGGCCTGTGGC TCCGGCGAGCAGGAGCTCAGGGCCATCCTCCGCGACCTGGGCTGCGGGCCCTACTTCCTGGGCACCTTCG ACAAGCGCTTCCCGGGATTCATGGCCCCGCACAAGCTGGCCTGCGCCATCGTCAACACGGCCGGCCGCGA GACCGGGGGCGAGCACTGGCTGGCCTTCGCCTGGAACCCGCGCACCCACACCTGCTACCTCTTCGACCCC TTCGGGTTCTCGGACGAGCGCCTCAAGCAGATCTACCAGTTCGAGTACGAGGGCCTGCTGCGCCGCAGCG CCCTGGCCACCGAGGACCGCTGCGTCACCCTGGAAAAGTCCACCCAGACCGTGCAGGGTCCGCGCTCGGC CGCCTGCGGGCTCTTCTGCTGCATGTTCCTGCACGCCTTCGTGCACTGGCCCGACCGCCCCATGGACAAG AACCCCACCATGAACTTGCTGACGGGGGTGCCCAACGGCATGCTCCAGTCGCCCCAGGTGGAACCCACCC TGCGCCGCAACCAGGAGGCGCTCTACCGCTTCCTCAACGCCCACTCCGCCTACTTTCGCTCCCACCGCGC GCGCATCGAGAAGGCCACCGCCTTCGACCGCATGAATCAAGACATGTAAACTGTGTGTATGTGAATGCTT TATTCATAATAAACAGCACATGTTTATGCCACCTTCTCTGAGGCTCTGACTTTATTTAGAAATCGAAGGG GTTCTGCCGGCTCTCGGCGTGCCCCGCGGGCAGGGATACGTTGCGGAACTGGTACTTGGGCAGCCACTTG AACTCGGGGATCAGCAGCTTCGGCACGGGGAGGTCGGGGAACGAGTCGCTCCACAGCTTGCGCGTGAGTT GCAGGGCGCCCAGCAGGTCGGGCGCGGATATCTTGAAATCACAGTTGGGACCCGCGTTCTGCGCGCGAGA GTTGCGGTACACGGGGTTGCAGCACTGGAACACCATCAGGGCCGGGTGCTTCACGCTCGCCAGCACCGTC GCGTCGGTGATGCCCTCCACGTCCAGATCCTCGGCGTTGGCCATCCCGAAGGGGGTCATCTTGCAGGTCT GCCGCCCCATGCTGGGCACGCAGCCGGGCTTGTGGTTGCAATCGCAGTGCAGGGGGATCAGCATCATCTG GGCCTGCTCGGAGCTCATGCCCGGGTACATGGCCTTCATGAAAGCCTCCAGCTGGCGGAAGGCCTGCTGC GCCTTGCCGCCCTCGGTGAAGAAGACCCCGCAGGACTTGCTAGAGAACTGGTTGGTGGCGCAGCCGGCGT CGTGCACGCAGCAGCGCGCGTCGTTGTTGGCCAGCTGCACCACGCTGCGCCCCCAGCGGTTCTGGGTGAT CTTGGCCCGGTCGGGGTTCTCCTTCAGCGCGCGCTGCCCGTTCTCGCTCGCCACATCCATCTCGATCGTG TGCTCCTTCTGGATCATCACGGTCCCGTGCAGGCACCGCAGCTTGCCCTCGGCTTCGGTGCATCCGTGCA GCCACAGCGCGCAGCCGGTGCACTCCCAGTTCTTGTGGGCGATCTGGGAGTGCGAGTGCACGAAGCCCTG CAGGAAGCGGCCCATCATCGCGGTCAGGGTCTTGTTGCTGGTGAAGGTCAGCGGGATGCCGCGGTGCTCC TCGTTCACATACAGGTGGCAGATGCGGCGGTACACCTCGCCCTGCTCGGGCATCAGCTGGAAGGCGGACT TCAGGTCGCTCTCCACGCGGTACCGCTCCATCAGCAGCGTCATGACTTCCATGCCCTTCTCCCAGGCCGA AACGATCGGCAGGCTCAGGGGGTTCTTCACCGTTGTCATCTTAGTCGCCGCCGCCGAGGTCAGGGGGTCG TTCTCGTCCAGGGTCTCAAACACTCGCTTGCCGTCCTTCTCGGTGATGCGCACGGGGGGAAAGCTGAAGC CCACGGCCGCCAGCTCCTCCTCGGCCTGCCTTTCGTCCTCGCTGTCCTGGCTGATGTCTTGCAAAGGCAC ATGCTTGGTCTTGCGGGGTTTCTTTTTGGGCGGCAGAGGCGGCGGCGGAGACGTGCTGGGCGAGCGCGAG TTCTCGCTCACCACGACTATTTCTTCTTCTTGGCCGTCGTCCGAGACCACGCGGCGGTAGGCATGCCTCT TCTGGGGCAGAGGCGGAGGCGACGGGCTCTCGCGGTTCGGCGGGCGGCTGGCAGAGCCCCTTCCGCGTTC GGGGGTGCGCTCCTGGCGGCGCTGCTCTGACTGACTTCCTCCGCGGCCGGCCATTGTGTTCTCCTAGGGA GCAAGCATGGAGACTCAGCCATCGTCGCCAACATCGCCATCTGCCCCCGCCGCCGCCGACGAGAACCAGC AGCAGCAGAATGAAAGCTTAACCGCCCCGCCGCCCAGCCCCACCTCCGACGCCGCGGCCCCAGACATGCA AGAGATGGAGGAATCCATCGAGATTGACCTGGGCTACGTGACGCCCGCGGAGCACGAGGAGGAGCTGGCA GCGCGCTTTTCAGCCCCGGAAGAGAACCACCAAGAGCAGCCAGAGCAGGAAGCAGAGAGCGAGCAGAGCC AGGCTGGGCTCGAGCATGGCGACTACCTGAGCGGGGCAGAGGACGTGCTCATCAAGCATCTGGCCCGCCA ATGCATCATCGTCAAGGATGCGCTGCTCGACCGCGCCGAGGTGCCCCTCAGCGTGGCGGAGCTCAGCCGC GCCTACGAGCGCAACCTCTTCTCGCCGCGCGTGCCCCCCAAGCGCCAGCCCAACGGCACCTGCGAGCCCA ACCCGCGCCTCAACTTCTACCCGGTCTTCGCGGTGCCCGAGGCCCTGGCCACCTACCACCTCTTTTTCAA GAACCAAAGGATCCCCGTCTCCTGCCGCGCCAACCGCACCCGCGCCGACGCCCTGCTCAACCTGGGCCCC GGCGCCCGCCTACCTGATATCGCCTCCTTGGAAGAGGTTCCCAAGATCTTCGAGGGTCTGGGCAGCGACG AGACTCGGGCCGCGAACGCTCTGCAAGGAAGCGGAGAGGAGCATGAGCACCACAGCGCCCTGGTGGAGTT GGAAGGCGACAACGCGCGCCTGGCGGTCCTCAAGCGCACGGTCGAGCTGACCCACTTCGCCTACCCGGCG CTCAACCTGCCCCCCAAGGTCATGAGCGCCGTCATGGACCAGGTGCTCATCAAGCGCGCCTCGCCCCTCT CGGAGGAGGAGATGCAGGACCCCGAGAGCTCGGACGAGGGCAAGCCCGTGGTCAGCGACGAGCAGCTGGC GCGCTGGCTGGGAGCGAGTAGCACCCCCCAGAGCCTGGAAGAGCGGCGCAAGCTCATGATGGCCGTGGTC CTGGTGACCGTGGAGCTGGAGTGTCTGCGCCGCTTCTTCGCCGACGCGGAGACCCTGCGCAAGGTCGAGG AGAACCTGCACTACCTCTTCAGGCACGGGTTCGTGCGCCAGGCCTGCAAGATCTCCAACGTGGAGCTGAC CAACCTGGTCTCCTACATGGGCATCCTGCACGAGAACCGCCTGGGGCAGAACGTGCTGCACACCACCCTG CGCGGGGAGGCCCGCCGCGACTACATCCGCGACTGCGTCTACCTGTACCTCTGCCACACCTGGCAGACGG GCATGGGCGTGTGGCAGCAGTGCCTGGAGGAGCAGAACCTGAAAGAGCTCTGCAAGCTCCTGCAGAAGAA CCTGAAGGCCCTGTGGACCGGGTTCGACGAGCGCACCACCGCCTCGGACCTGGCCGACCTCATCTTCCCC GAGCGCCTGCGGCTGACGCTGCGCAACGGGCTGCCCGACTTTATGAGCCAAAGCATGTTGCAAAACTTTC GCTCTTTCATCCTCGAACGCTCCGGGATCCTGCCCGCCACCTGCTCCGCGCTGCCCTCGGACTTCGTGCC GCTGACCTTCCGCGAGTGCCCCCCGCCGCTCTGGAGCCACTGCTACCTGCTGCGTCTGGCCAACTACCTG GCCTACCACTCGGACGTGATCGAGGACGTCAGCGGCGAGGGTCTGCTCGAGTGCCACTGCCGCTGCAACC TCTGCACGCCGCACCGCTCCCTGGCCTGCAACCCCCAGCTGCTGAGCGAGACCCAGATCATCGGCACCTT CGAGTTGCAAGGCCCCGGCGAGGAGGGCAAGGGGGGTCTGAAACTCACCCCGGGGCTGTGGACCTCGGCC TACTTGCGCAAGTTCGTGCCCGAGGACTACCATCCCTTCGAGATCAGGTTCTACGAGGACCAATCCCAGC CGCCCAAGGCCGAGCTGTCGGCCTGCGTCATCACCCAGGGGGCCATCCTGGCCCAATTGCAAGCCATCCA GAAATCCCGCCAAGAATTTCTGCTGAAAAAGGGCCACGGGGTCTACTTGGACCCCCAGACCGGAGAGGAG CTCAACCCCAGCTTCCCCCAGGATGCCCAGAGGAAGCAGCAAGAAGCTGAAAGTGGAGCTGCCGCTGCCG CCGGAGGATTTGGAGGAAGACTGGGAGAGCAGTCAGGCAGAGGAGGAGGAGATGGAAGACTGGGACAGCA CTCAGGCAGAGGAGGACAGCCTGCAAGACAGTCTGGAAGACGAGGTGGAGGAGGAGGCAGAGGAAGAAGC AGCCGCCGCCAGACCGTCGTCCTCGGCGGAGAAAGCAAGCAGCACGGATACCATCTCCGCTCCGGGTCGG GGTCTCGGCGGCCGGGCCCACAGTAGGTGGGACGAGACCGGGCGCTTCCCGAACCCCACCACCCAGACCG GTAAGAAGGAGCGGCAGGGATACAAGTCCTGGCGGGGGCACAAAAACGCCATCGTCTCCTGCTTGCAAGC CTGCGGGGGCAACATCTCCTTCACCCGGCGCTACCTGCTCTTCCACCGCGGGGTGAACTTCCCCCGCAAC ATCTTGCATTACTACCGTCACCTCCACAGCCCCTACTACTGTTTCCAAGAAGAGGCAGAAACCCAGCAGC AGCAGAAAACCAGCAGCAGCTAGAAAATCCACAGCGGCGGCGGCGGCAGGTGGACTGAGGATCGCGGCGA ACGAGCCGGCGCAGACCCGGGAGCTGAGGAACCGGATCTTTCCCACCCTCTATGCCATCTTCCAGCAGAG TCGGGGGCAGGAGCAGGAACTGAAAGTCAAGAACCGTTCTCTGCGCTCGCTCACCCGCAGTTGTCTGTAT CACAAGAGCGAAGACCAACTTCAGCGCACTCTCGAGGACGCCGAGGCTCTCTTCAACAAGTACTGCGCGC TCACTCTTAAAGAGTAGCCCGCGCCCGCCCACACACGGAAAAAGGCGGGAATTACGTCACCACCTGCGCC CTTCGCCCGACCATCATCATGAGCAAAGAGATTCCCACGCCTTACATGTGGAGCTACCAGCCCCAGATGG GCCTGGCCGCCGGCGCCGCCCAGGACTACTCCACCCGCATGAACTGGCTCAGTGCCGGGCCCGCGATGAT CTCACGGGTGAATGACATCCGCGCCCGCCGAAACCAGATACTCCTAGAACAGTCAGCGATCACCGCCACG CCCCGCCATCACCTTAATCCGCGTAATTGGCCCGCCGCCCTGGTGTACCAGGAAATTCCCCAGCCCACGA CCGTACTACTTCCGCGAGACGCCCAGGCCGAAGTCCAGCTGACTAACTCAGGTGTCCAGCTGGCCGGCGG CGCCGCCCTGTGTCGTCACCGCCCCGCTCAGGGTATAAAGCGGCTGGTGATCCGAGGCAGAGGCACACAG CTCAACGACGAGGTGGTGAGCTCTTCGCTGGGTCTGCGACCTGACGGAGTCTTCCAACTCGCCGGATCGG GGAGATCTTCCTTCACGCCTCGTCAGGCCGTCCTGACTTTGGAGAGTTCGTCCTCGCAGCCCCGCTCGGG TGGCATCGGCACTCTCCAGTTCGTGGAGGAGTTCACTCCCTCGGTCTACTTCAACCCCTTCTCCGGCTCC CCCGGCCACTACCCGGACGAGTTCATCCCGAACTTCGACGCCATCAGCGAGTCGGTGGACGGCTACGATT GAATGTCCCATGGTGGCGCGGCTGACCTAGCTCGGCTTCGACACCTGGACCACTGCCGCCGCTTCCGCTG CTTCGCTCGGGATCTCGCCGAGTTTGCCTACTTTGAGCTGCCCGAGGAGCACCCTCAGGGCCCGGCCCAC GGAGTGCGGATCATCGTCGAAGGGGGCCTCGACTCCCACCTGCTTCGGATCTTCAGCCAGCGTCCGATCC

TGGTCGAGCGCGAGCAAGGACAGACCCGTCTGACCCTGTACTGCATCTGCAACCACCCCGGCCTGCATGA AAGTCTTTGTTGTCTGCTGTGTACTGAGTATAATAAAAGCTGAGATCAGCGACTACTCCGGACTTCCGTG TGTTCCTGAATCCATCAACCAGTCCCTGTTCTTCACCGGGAACGAGACCGAGCTCCAGCTCCAGTGTAAG CCCCACAAGAAGTACCTCACCTGGCTGTTCCAGGGCTCCCCGATCGCCGTTGTCAACCACTGCGACAACG ACGGAGTCCTGCTGAGCGGCCCTGCCAACCTTACTTTTTCCACCCGCAGAAGCAAGCTCCAGCTCTTCCA ACCCTTCCTCCCCGGGACCTATCAGTGCGTCTCGGGACCCTGCCATCACACCTTCCACCTGATCCCGAAT ACCACAGCGTCGCTCCCCGCTACTAACAACCAAACTACCCACCAACGCCACCGTCGCGACCTTTCCTCTG AATCTAATACCACTACCGGAGGTGAGCTCCGAGGTCGACCAACCTCTGGGATTTACTACGGCCCCTGGGA GGTGGTGGGGTTAATAGCGCTAGGCCTAGTTGTGGGTGGGCTTTTGGCTCTCTGCTACCTATACCTCCCT TGCTGTTCGTACTTAGTGGTGCTGTGTTGCTGGTTTAAGAAATGGGGCAGATCACCCTAGTGAGCTGCGG TGTGCTGGTGGCGGTGGTGCTTTCGATTGTGGGACTGGGCGGCGCGGCTGTAGTGAAGGAGAAGGCCGAT CCCTGCTTGCATTTCAATCCCGACAAATGCCAGCTGAGTTTTCAGCCCGATGGCAATCGGTGCGCGGTGC TGATCAAGTGCGGATGGGAATGCGAGAACGTGAGAATCGAGTACAATAACAAGACTCGGAACAATACTCT CGCGTCCGTGTGGCAGCCCGGGGACCCCGAGTGGTACACCGTCTCTGTCCCCGGTGCTGACGGCTCCCCG CGCACCGTGAATAATACTTTCATTTTTGCGCACATGTGCGACACGGTCATGTGGATGAGCAAGCAGTACG ATATGTGGCCCCCCACGAAGGAGAACATCGTGGTCTTCTCCATCGCTTACAGCCTGTGCACGGTGCTAAT CACCGCTATCGTGTGCCTGAGCATTCACATGCTCATCGCTATTCGCCCCAGAAATAATGCCGAAAAAGAG AAACAGCCATAACACGTTTTTTCACACACCTTGTTTTTACAGACAATGCGTCTGTTAAATTTTTTAAACA TTGTGCTCAGTATTGCTTATGCCTCTGGCTATGCAAACATACAGAAAACCCTCTATGTAGGATCTGATGA TACACTAGAGGGTACCCAATCACAAGCTAGGGTTTCATGGTATTTTTATAAAAGCTCAGATAATCCTATT ACTCTTTGCAAAGGTGATCAGGGGCGGACAACAAAGCCGCCTATCACATTTAGCTGTACCAGAACAAATC TCACGCTTTTCTCAATTACAAAACAATATGCTGGTATTTATTACAGTACAAACTTTCATAGTGGGCAAGA TAAATATTATACTGTTAAGGTAGAAAATCCTACCACTCCTAGAACTACCACCACCACCACCACCACCACC ACTACTGCGAAGCCCACTAAACCTAAAACTACCAAGAAAACCACTGTGAAAACTACAACTAGAACCACCA CAACTACAGAAACCACCACCAGCACAACACTTGCTGCAACTACACACACACACACTGAGCTAACCTTACA GACCACTAATGATTTGATAGCCCTGTTGCAAAAGGGGGATAACAGCACCACTTCCAATGAGGAGATACCC AAATCCATGATTGGCATTATTGTTGCTGTAGTGGTGTGCATGTTGATCATCGCCTTGTGCATGGTGTACT ATGCCTTCTGCTACAGAAAGCACAGACTGAACGACAAGCTGGAACACTTACTAAGTGTTGAATTTTAATT TTTTAGAACCATGAAGATCCTAGGCCTTTTAGTTTTTTCTATCATTACCTCTGCTCTATGCAATTCTGAC AATGAGGACGTTACTGTCGTTGTCGGATCAAATTATACACTGAAAGGTCCAGCGAAGGGTATGCTTTCGT GGTATTGCTGGTTTGGAACTGACACTGATCAAACTGAGCTTTGCAATGCAATGAAAGGTCAAATACCAAC CTCAAAAATTAAACATAAATGCAATGGTACTGACTTAGTACTACTCAATATCACGAAATCATATGCTGGC AGCTATTCATGCCCTGGAGATGATGCTGAGAACATGATTTTTTACAAAGTAACTGTTGTTGATCCCACTA CTCCACCACCCACCACCACAACTACTCACACCACACACACAGAACAAACACCAGAGGCAGCAGAAGCAGA GTTGGCCTTCCAGGTTCACGGAGATTCCTTTGCTGTCAATACCCCTACACCCGATCATCGGTGTCCGGGG CTGCTAGTCAGCGGCATTGTCGGTGTGCTTTCGGGATTAGCAGTCATAATCATCTGCATGTTCATTTTTG CTTGCTGCTATAGAAGGCTTTACCGACAAAAATCAGACCCACTGCTGAACCTCTATGTTTAATTTTTTCC AGAGCCATGAAGGCAGTTAGCGCTCTAGTTTTTTGTTCTTTGATTGGCATTGTTTTTTGCAATCCTATTA CTAGAGTTAGCTTTATTAAAGATGTGAATGTTACTGAGGGGGGCAATGTGACACTGGTAGGTGTAGAGGG TGCTAAAAACACCACCTGGACAAAATACCACCTTGGGTGGAAAGATATTTGCAATTGGAGTGTCACTGTG TACACATGTGAGGGAGTTAATCTTACCATTGTCAATGCCACCTCAGCTCAAAATGGTAGAATTCAAGGAC AAAGTGTTAGTGTGACCAGTGATGGGTATTTTACCCAACATACTTTTATCTATGACGTTAAAGTCATACC ACTGCCTACGCCTAGCCCACCTAGCACCACTACACAAACAACCCACACTACACAGACAACCACATACAGT ACATCAAATCAGCCTACCACCACTACAGCAGCAGAGGTTGCCAGCTCGTCTGGAGTTCAAGTGGCATTTT TGTTGTTGCCCCCATCTAGCAGTCCCACTGCTATTACCAATGAGCAGACTACTGCATTTTTGTCCACTGT CGAGAGCCACACCACAGCTACCTCCAGTGCCTTCTCTAGCACCGCCAATCTCTCCTCGCTTTCCTCTACA CCAATCAGTCCCGCTACTACTACTACCCCCGCTATTCTTCCCACTCCCCTGAAGCAAACAGACGGCGGCA TGCAATGGCAGATCACCCTGCTCATTGTGATCGGGTTGGTCATCCTAGCCGTGTTGCTCTACTACATCTT CTGCCGCCGCATTCCCAACGCGCACCGCAAGCCGGTCTACAAGCCCATCATTGTCGGGCAGCCGGAGCCG CTTCAGGTGGAAGGGGGTCTAAGGAATCTTCTCTTCTCTTTTACAGTATGGTGATTGAACTATGATTCCT AGACAATTCTTGATCACTATTCTTATCTGCCTCCTCCAAGTCTGTGCCACCCTCGCTCTGGTGGCCAACG CCAGTCCAGACTGTATTGGGCCCTTCGCCTCCTACGTGCTCTTTGCCTTCATCACCTGCATCTGCTGCTG TAGCATAGTCTGCCTGCTTATCACCTTCTTCCAGTTCATTGACTGGATCTTTGTGCGCATCGCCTACCTG CGCCACCACCCCCAGTACCGCGACCAGCGAGTGGCGCAGCTGCTCAGGCTCCTCTGATAAGCATGCGGGC TCTGCTACTTCTCGCGCTTCTGCTGTTAGTGCTCCCCCGTCCCGTTGACCCCCGGCCCCCCACTCAGTCC CCCGAGGAGGTCCGCAAATGCAAATTCCAAGAACCCTGGAAATTCCTCAAATGCTACCGCCAAAAATCAG ACATGCATCCCAGCTGGATCATGATCATTGGGATCGTGAACATTCTGGCCTGCACCCTCATCTCCTTTGT GATTTACCCCTGCTTTGACTTTGGTTGGAACTCGCCAGAGGCGCTCTATCTCCCGCCTGAACCTGACACA CCACCACAGCAACCTCAGGCACACGCACTACCACCACCACAGCCTAGGCCACAATACATGCCCATATTAG ACTATGAGGCCGAGCCACAGCGACCCATGCTCCCCGCTATTAGTTACTTCAATCTAACCGGCGGAGATGA CTGACCCACTGGCCAACAACAACGTCAACGACCTTCTCCTGGACATGGACGGCCGCGCCTCGGAGCAGCG ACTCGCCCAACTTCGCATTCGCCAGCAGCAGGAGAGAGCCGTCAAGGAGCTGCAGGACGGCATAGCCATC CACCAGTGCAAGAAAGGCATCTTCTGCCTGGTGAAACAGGCCAAGATCTCCTACGAGGTCACCCAGACCG ACCATCGCCTCTCCTACGAGCTCCTGCAGCAGCGCCAGAAGTTCACCTGCCTGGTCGGAGTCAACCCCAT CGTCATCACCCAGCAGTCGGGCGATACCAAGGGGTGCATCCACTGCTCCTGCGACTCCCCCGACTGCGTC CACACTCTGATCAAGACCCTCTGCGGCCTCCGCGACCTCCTCCCCATGAACTAATCACCCACTTATCCAG TGAAATAAAAAAATAATCATTTGATTTGAAATAAAGATACAATCATATTGATGATTTGAGTTTAACAAAA ATAAAGAATCACTTACTTGAAATCTGATACCAGGTCTCTGTCCATATTTTCTGCCAACACCACCTCACTC CCCTCTTCCCAGCTCTGGTACTGCAGGCCCCGGCGGGCTGCAAACTTCCTCCACACGCTGAAGGGGATGT CAAATTCCTCCTGCCCCTCAATCTTCATTTTATCTTCTATCAGATGTCCAAAAAGCGCGTCCGGGTGGAT GATGACTTCGACCCCGTCTACCCCTACGATGCAGACAACGCACCGACCGTGCCCTTCATCAACCCCCCCT TCGTCTCTTCAGATGGATTCCAAGAGAAGCCCCTGGGGGTGTTGTCCCTGCGACTGGCCGACCCCGTCAC CACCAAGAACGGGGAAATCACCCTCAAGCTGGGAGAGGGGGTGGACCTCGACTCCTCGGGAAAACTCATC TCCAACACGGCCACCAAGGCCGCTGCCCCTCTCAGTTTTTCCAACAACACCATTTCCCTTAACATGGATC ACCCCTTTTACACTAAAGATGGAAAATTAGCCTTACAAGTTTCTCCACCATTAAATATACTGAGAACAAG CATTCTAAACACACTAGCTTTAGGTTTTGGATCAGGTTTAGGACTCCGTGGCTCTGCCTTGGCAGTACAG TTAGTCTCTCCACTTACATTTGATACTGATGGAAACATAAAGCTTACCTTAGACAGAGGTTTGCATGTTA CAACAGGAGATGCAATTGAAAGCAACATAAGCTGGGCTAAAGGTTTAAAATTTGAAGATGGAGCCATAGC AACCAACATTGGAAATGGGTTAGAGTTTGGAAGCAGTAGTACAGAAACAGGTGTCGATGATGCTTACCCA ATCCAAGTTAAACTTGGATCTGGCCTTAGCTTTGACAGTACAGGAGCCATAATGGCTGGTAACAAAGAAG ACGATAAACTCACTTTGTGGACAACACCTGATCCATCACCAAACTGTCAAATACTCGCAGAAAATGATGC AAAACTAACACTTTGCTTGACTAAATGTGGTAGTCAAATACTGGCCACTGTGTCAGTCTTAGTTGTAGGA AGTGGAAACCTAAACCCCATTACTGGCACCGTAAGCAGTGCTCAGGTGTTTCTACGTTTTGATGCAAACG GTGTTCTTTTAACAGAACATTCTACACTAAAAAAATACTGGGGGTATAGGCAGGGAGATAGCATAGATGG CACTCCATATGTCAATGCTGTAGGATTCATGCCCAATTTAAAAGCTTATCCAAAGTCACAAAGTTCTACT ACTAAAAATAATATAGTAGGGCAAGTATACATGAATGGAGATGTTTCAAAACCTATGCTTCTCACTATAA CCCTCAATGGTACTGATGACAGCAACAGTACATATTCAATGTCATTTTCATACACCTGGACTAATGGAAG CTATGTTGGAGCAACATTTGGAGCTAACTCTTATACCTTCTCCTACATCGCCCAAGAATGAATACTGTAT CCCACCCTGCATGCCCAACCCTCCCCCACCTCTGTCTATATGGAAAACTCTGAAACACAAAATAAAATAA AGTTCAAGTGTTTTATTGATTCAACAGTTTTACAGGATTCGAGCAGTTATTTTTCCTCCACCCTCCCAGG ACATGGAATACACCACCCTCTCCCCCCGCACAGCCTTGAACATCTGAATGCCATTGGTGATGGACATGCT TTTGGTCTCCACGTTCCACACAGTTTCAGAGCGAGCCAGTCTCGGGTCGGTCAGGGAGATGAAACCCTCC GGGCACTCCCGCATCTGCACCTCACAGCTCAACAGCTGAGGATTGTCCTCGGTGGTCGGGATCACGGTTA TCTGGAAGAAGCAGAAGAGCGGCGGTGGGAATCATAGTCCGCGAACGGGATCGGCCGGTGGTGTCGCATC AGGCCCCGCAGCAGTCGCTGCCGCCGCCGCTCCGTCAAGCTGCTGCTCAGGGGGTCCGGGTCCAGGGACT CCCTCAGCATGATGCCCACGGCCCTCAGCATCAGTCGTCTGGTGCGGCGGGCGCAGCAGCGCATGCGGAT CTCGCTCAGGTCGCTGCAGTACGTGCAACACAGGACCACCAGGTTGTTCAACAGTCCATAGTTCAACACG CTCCAGCCGAAACTCATCGCGGGAAGGATGCTACCCACGTGGCCGTCGTACCAGATCCTCAGGTAAATCA AGTGGCGCCCCCTCCAGAACACGCTGCCCATGTACATGATCTCCTTGGGCATGTGGCGGTTCACCACCTC CCGGTACCACATCACCCTCTGGTTGAACATGCAGCCCCGGATGATCCTGCGGAACCACAGGGCCAGCACC GCCCCGCCCGCCATGCAGCGAAGAGACCCCGGGTCCCGGCAATGGCAATGGAGGACCCACCGCTCGTACC CGTGGATCATCTGGGAGCTGAACAAGTCTATGTTGGCACAGCACAGGCACACGCTCATGCATCTCTTCAG CACTCTCAGCTCCTCGGGGGTCAAAACCATATCCCAGGGCACGGGAAACTCTTGCAGGACAGCGAAGCCC GCAGAACAGGGCAATCCTCGCACATAACTTACATTGTGCATGGACAGGGTATCGCAATCAGGCAGCACCG GGTGATCCTCCACCAGAGAAGCGCGGGTCTCGGTCTCCTCACAGCGTGGTAAGGGGGCCGGCCGATACGG GTGATGGCGGGACGCGGCTGATCGTGTTCGCGACCGTGTCATGATGCAGTTGCTTTCGGACATTTTCGTA CTTGCTGAAGCAGAACCTGGTCCGGGCGCTGCACACCGATCGCCGGCGGCGGTCTCGGCGCTTGGAACGC TCGGTGTTGAAGTTGTAAAACAGCCACTCTCTCAGACCGTGCAGCAGATCTAGGGCCTCAGGAGTGATGA AGATCCCATCATGCCTGATGGCTCTGATCACATCGACCACCGTGGAATGGGCCAGACCCAGCCAGATGAT GCAATTTTGTTGGGTTTCGGTGACGGCGGGGGAGGGAAGAACAGGAAGAACCATGATTAACTTTTAATCC AAACGGTCTCGGAGCACTTCAAAATGAAGGTCGCGGAGATGGCACCTCTCGCCCCCGCTGTGTTGGTGGA AAATAACAGCCAGGTCAAAGGTGATACGGTTCTCGAGATGTTCCACGGTGGCTTCCAGCAAAGCCTCCAC GCGCACATCCAGAAACAAGACAATAGCGAAAGCGGGAGGGTTCTCTAATTCCTCAATCATCATGTTACAC TCCTGCACCATCCCCAGATAATTTTCATTTTTCCAGCCTTGAATGATTCGAACTAGTTCCTGAGGTAAAT CCAAGCCAGCCATGATAAAGAGCTCGCGCAGAGCGCCCTCCACCGGCATTCTTAAGCACACCCTCATAAT TCCAAGATATTCTGCTCCTGGTTCACCTGCAGCAGATTGACAAGCGGGATATCAAAATCTCTGCCGCGAT CCCTGAGCTCCTCCCTCAGCAATAACTGTAAGTACTCTTTCATATCCTCTCCGAAATTTTTAGCCATAGG ACCCCCAGGAATAAGAGAAGGGCAAGCCACATTACAGATAAACCGAAGTCCCCCCCAGTGAGCATTGCCA AATGTAAGATTGAAATAAGCATGCTGGCTAGACCCGGTGATATCTTCCAGATAACTGGACAGAAAATCGG GCAAGCAATTTTTAAGAAAATCAACAAAAGAAAAATCTTCCAGGTGCACGTTTAGGGCCTCGGGAACAAC GATGGAGTAAGTGCAAGGGGTGCGTTCCAGCATGGTTAGTTAGCTGATCTGTAAAAAAACAAAAAATAAA ACATTAAACCATGCTAGCCTGGCGAACAGGTGGGTAAATCGTTCTCTCCAGCACCAGGCAGGCCACGGGG TCTCCGGCGCGACCCTCGTAAAAATTGTCGCTATGATTGAAAACCATCACAGAGAGACGTTCCCGGTGGC CGGCGTGAATGATTCGAGAAGAAGCATACACCCCCGGAACATTGGAGTCCGTGAGTGAAAAAAAGCGGCC GAGGAAGCAATGAGGCACTACAACGCTCACTCTCAAGTCCAGCAAAGCGATGCCATGCGGATGAAGCACA

AAATTTTCAGGTGCGTAAAAAATGTAATTACTCCCCTCCTGCACAGGCAGCGAAGCTCCCGATCCCTCCA GATACACATACAAAGCCTCAGCGTCCATAGCTTACCGAGCGGCAGCAGCAGCGGCACACAACAGGCGCAA GAGTCAGAGAAAAGACTGAGCTCTAACCTGTCCGCCCGCTCTCTGCTCAATATATAGCCCCAGATCTACA CTGACGTAAAGGCCAAAGTCTAAAAATACCCGCCAAATAATCACACACGCCCAGCACACGCCCAGAAACC GGTGACACACTCAAAAAAATACGCGCACTTCCTCAAACGCCCAAACTGCCGTCATTTCCGGGTTCCCACG CTACGTCATCAAAACACGACTTTCAAATTCCGTCGACCGTTAAAAACGTCACCCGCCCCGCCCCTAACGG TCGCCGCTCCCGCAGCCAATCAGCGCCCCGCATCCCCAAATTCAAACAGCTCATTTGCATATTAACGCGC ACCAAAAGTTTGAGGTATATTATTGATGATG Polynucleotide sequence encoding the CASI promoter SEQ ID NO: 3 GGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGT- CAA TAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAA- ACT GCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCC- CGC CTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTA- TTA CCATGGTCGAGGTGAGCCCCACGTTCTGCTTCACTCTCCCCATCTCCCCCCCCTCCCCACCCCCAATTTTGTAT- TTA TTTATTTTTTAATTATTTTGTGCAGCGATGGGGGCGGGGGGGGGGGGGGGCGCGCGCCAGGCGGGGCGGGGCGG- GGC GAGGGGCGGGGCGGGGCGAGGCGGAGAGGTGCGGCGGCAGCCAATCAGAGCGGCGCGCTCCGAAAGTTTCCTTT- TAT GGCGAGGCGGCGGCGGCGGCGGCCCTATAAAAAGCGAAGCGCTCCCTATCAGTGATAGAGATCTCCCTATCAGT- GAT AGAGATCGTCGACGAGCTCGCGGCGGGCGGGAGTCGCTGCGCGCTGCCTTCGCCCCGTGCCCCGCTCCGCCGCC- GCC TCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTAAAACAGGTAAGTCCGGCCTCCGCGCCGGGTTTTGG- CGC CTCCCGCGGGCGCCCCCCTCCTCACGGCGAGCGCTGCCACGTCAGACGAAGGGCGCAGCGAGCGTCCTGATCCT- TCC GCCCGGACGCTCAGGACAGCGGCCCGCTGCTCATAAGACTCGGCCTTAGAACCCCAGTATCAGCAGAAGGACAT- TTT AGGACGGGACTTGGGTGACTCTAGGGCACTGGTTTTCTTTCCAGAGAGCGGAACAGGCGAGGAAAAGTAGTCCC- TTC TCGGCGATTCTGCGGAGGGATCTCCGTGGGGCGGTGAACGCCGATGATGCCTCTACTAACCATGTTCATGTTTT- CTT TTTTTTTCTACAGGTCCTGGGTGACGAACAG Polynucleotide sequence encoding ChAd155/RSV SEQ ID NO: 4 CATCATCAATAATATACCTTATTTTGGATTGAAGCCAATATGATAATGAGATGGGCGGCGCGGGGCGG GGCGCGGGGCGGGAGGCGGGTTTGGGGGCGGGCCGGCGGGCGGGGCGGTGTGGCGGAAGTGGACTTT GTAAGTGTGGCGGATGTGACTTGCTAGTGCCGGGCGCGGTAAAAGTGACGTTTTCCGTGCGCGACAAC GCCCCCGGGAAGTGACATTTTTCCCGCGGTTTTTACCGGATGTTGTAGTGAATTTGGGCGTAACCAAGT AAGATTTGGCCATTTTCGCGGGAAAACTGAAACGGGGAAGTGAAATCTGATTAATTTTGCGTTAGTCA TACCGCGTAATATTTGTCTAGGGCCGAGGGACTTTGGCCGATTACGTGGAGGACTCGCCCAGGTGTTTT TTGAGGTGAATTTCCGCGTTCCGGGTCAAAGTCTGCGTTTTATTATTATAGGATATCCCATTGCATACG TTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTGACATTGAT TATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCG TTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATA ATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACG GTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATG ACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACA TCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGC GGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAA ATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTA CGGTGGGAGGTCTATATAAGCAGAGCTCTCCCTATCAGTGATAGAGATCTCCCTATCAGTGATAGAGA TCGTCGACGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCA TAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCGGGAACGGTGCATTGGAACGCGGATTCCCCGT GCCAAGAGTGAGATCTTCCGTTTATCTAGGTACCAGATATCGCCACCATGGAACTGCTGATCCTGAAG GCCAACGCCATCACCACCATCCTGACCGCCGTGACCTTCTGCTTCGCCAGCGGCCAGAACATCACCGA GGAATTCTACCAGAGCACCTGTAGCGCCGTGAGCAAGGGCTACCTGAGCGCCCTGAGAACCGGCTGGT ACACCAGCGTGATCACCATCGAGCTGAGCAACATCAAAGAAAACAAGTGCAACGGCACCGACGCCAA AGTGAAGCTGATCAAGCAGGAACTGGACAAGTACAAGAACGCCGTGACCGAGCTGCAGCTGCTGATG CAGAGCACCCCCGCCACCAACAACCGGGCCAGACGGGAGCTGCCCCGGTTCATGAACTACACCCTGA ACAACGCCAAAAAGACCAACGTGACCCTGAGCAAGAAGCGGAAGCGGCGGTTCCTGGGCTTTCTGCT GGGCGTGGGCAGCGCCATTGCCAGCGGCGTGGCCGTGTCTAAGGTGCTGCACCTGGAAGGCGAAGTG AACAAGATCAAGAGCGCCCTGCTGAGCACCAACAAGGCCGTGGTGTCCCTGAGCAACGGCGTGAGCG TGCTGACCAGCAAGGTGCTGGATCTGAAGAACTACATCGACAAGCAGCTGCTGCCCATCGTGAACAAG CAGAGCTGCAGCATCAGCAACATCGAGACAGTGATCGAGTTCCAGCAGAAGAACAACCGGCTGCTGG AAATCACCCGGGAGTTCAGCGTGAACGCCGGCGTGACCACCCCTGTGTCCACCTACATGCTGACCAAC AGCGAGCTGCTGAGCCTGATCAACGACATGCCCATCACCAACGACCAGAAAAAGCTGATGAGCAACA ACGTGCAGATCGTGCGGCAGCAGAGCTACTCCATCATGTCCATCATCAAAGAAGAGGTGCTGGCCTAC GTGGTGCAGCTGCCCCTGTACGGCGTGATCGACACCCCCTGCTGGAAGCTGCACACCAGCCCCCTGTG CACCACCAACACCAAAGAGGGCAGCAACATCTGCCTGACCCGGACCGACAGAGGCTGGTACTGCGAC AACGCCGGCAGCGTGTCATTCTTTCCACAGGCCGAGACATGCAAGGTGCAGAGCAACCGGGTGTTCTG CGACACCATGAACAGCCTGACCCTGCCCTCCGAAGTGAACCTGTGCAACGTGGACATCTTCAACCCCA AGTACGACTGCAAGATCATGACCTCCAAGACCGACGTGTCCAGCTCCGTGATCACCTCCCTGGGCGCC ATCGTGTCCTGCTACGGCAAGACCAAGTGCACCGCCAGCAACAAGAACCGGGGCATCATCAAGACCTT CAGCAACGGCTGCGACTACGTGTCCAACAAGGGGGTGGACACCGTGTCCGTGGGCAACACCCTGTACT ACGTGAACAAACAGGAAGGCAAGAGCCTGTACGTGAAGGGCGAGCCCATCATCAACTTCTACGACCC CCTGGTGTTCCCCAGCGACGAGTTCGACGCCAGCATCAGCCAGGTGAACGAGAAGATCAACCAGAGC CTGGCCTTCATCCGGAAGTCCGACGAGCTGCTGCACAATGTGAATGCCGGCAAGTCCACCACCAACCG GAAGCGGAGAGCCCCTGTGAAGCAGACCCTGAACTTCGACCTGCTGAAGCTGGCCGGCGACGTGGAG AGCAATCCCGGCCCTATGGCCCTGAGCAAAGTGAAACTGAACGATACACTGAACAAGGACCAGCTGC TGTCCAGCAGCAAGTACACCATCCAGCGGAGCACCGGCGACAGCATCGATACCCCCAACTACGACGT GCAGAAGCACATCAACAAGCTGTGCGGCATGCTGCTGATCACAGAGGACGCCAACCACAAGTTCACC GGCCTGATCGGCATGCTGTACGCCATGAGCCGGCTGGGCCGGGAGGACACCATCAAGATCCTGCGGG ACGCCGGCTACCACGTGAAGGCCAATGGCGTGGACGTGACCACACACCGGCAGGACATCAACGGCAA AGAAATGAAGTTCGAGGTGCTGACCCTGGCCAGCCTGACCACCGAGATCCAGATCAATATCGAGATCG AGAGCCGGAAGTCCTACAAGAAAATGCTGAAAGAAATGGGCGAGGTGGCCCCCGAGTACAGACACGA CAGCCCCGACTGCGGCATGATCATCCTGTGTATCGCCGCCCTGGTGATCACAAAGCTGGCCGCTGGCG ACAGATCTGGCCTGACAGCCGTGATCAGACGGGCCAACAATGTGCTGAAGAACGAGATGAAGCGGTA CAAGGGCCTGCTGCCCAAGGACATTGCCAACAGCTTCTACGAGGTGTTCGAGAAGTACCCCCACTTCA TCGACGTGTTCGTGCACTTCGGCATTGCCCAGAGCAGCACCAGAGGCGGCTCCAGAGTGGAGGGCATC TTCGCCGGCCTGTTCATGAACGCCTACGGCGCTGGCCAGGTGATGCTGAGATGGGGCGTGCTGGCCAA GAGCGTGAAGAACATCATGCTGGGCCACGCCAGCGTGCAGGCCGAGATGGAACAGGTGGTGGAGGTG TACGAGTACGCCCAGAAGCTGGGCGGAGAGGCCGGCTTCTACCACATCCTGAACAACCCTAAGGCCTC CCTGCTGTCCCTGACCCAGTTCCCCCACTTCTCCAGCGTGGTGCTGGGAAATGCCGCCGGACTGGGCAT CATGGGCGAGTACCGGGGCACCCCCAGAAACCAGGACCTGTACGACGCCGCCAAGGCCTACGCCGAG CAGCTGAAAGAAAACGGCGTGATCAACTACAGCGTGCTGGACCTGACCGCTGAGGAACTGGAAGCCA TCAAGCACCAGCTGAACCCCAAGGACAACGACGTGGAGCTGGGAGGCGGAGGATCTGGCGGCGGAGG CATGAGCAGACGGAACCCCTGCAAGTTCGAGATCCGGGGCCACTGCCTGAACGGCAAGCGGTGCCAC TTCAGCCACAACTACTTCGAGTGGCCCCCTCATGCTCTGCTGGTGCGGCAGAACTTCATGCTGAACCGG ATCCTGAAGTCCATGGACAAGAGCATCGACACCCTGAGCGAGATCAGCGGAGCCGCCGAGCTGGACA GAACCGAGGAATATGCCCTGGGCGTGGTGGGAGTGCTGGAAAGCTACATCGGCTCCATCAACAACAT CACAAAGCAGAGCGCCTGCGTGGCCATGAGCAAGCTGCTGACAGAGCTGAACAGCGACGACATCAAG AAGCTGAGGGACAACGAGGAACTGAACAGCCCCAAGATCCGGGTGTACAACACCGTGATCAGCTACA TTGAGAGCAACCGCAAGAACAACAAGCAGACCATCCATCTGCTGAAGCGGCTGCCCGCCGACGTGCT GAAAAAGACCATCAAGAACACCCTGGACATCCACAAGTCCATCACCATCAACAATCCCAAAGAAAGC ACCGTGTCTGACACCAACGATCACGCCAAGAACAACGACACCACCTGATGAGCGGCCGCGATCTGCTG TGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCAC TCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCT GGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGA TGCGGTGGGCTCTATGGCCGATCAGCGATCGCTGAGGTGGGTGAGTGGGCGTGGCCTGGGGTGGTCAT GAAAATATATAAGTTGGGGGTCTTAGGGTCTCTTTATTTGTGTTGCAGAGACCGCCGGAGCCATGAGC GGGAGCAGCAGCAGCAGCAGTAGCAGCAGCGCCTTGGATGGCAGCATCGTGAGCCCTTATTTGACGA CGCGGATGCCCCACTGGGCCGGGGTGCGTCAGAATGTGATGGGCTCCAGCATCGACGGCCGACCCGTC CTGCCCGCAAATTCCGCCACGCTGACCTATGCGACCGTCGCGGGGACGCCGTTGGACGCCACCGCCGC CGCCGCCGCCACCGCAGCCGCCTCGGCCGTGCGCAGCCTGGCCACGGACTTTGCATTCCTGGGACCAC TGGCGACAGGGGCTACTTCTCGGGCCGCTGCTGCCGCCGTTCGCGATGACAAGCTGACCGCCCTGCTG GCGCAGTTGGATGCGCTTACTCGGGAACTGGGTGACCTTTCTCAGCAGGTCATGGCCCTGCGCCAGCA GGTCTCCTCCCTGCAAGCTGGCGGGAATGCTTCTCCCACAAATGCCGTTTAAGATAAATAAAACCAGA CTCTGTTTGGATTAAAGAAAAGTAGCAAGTGCATTGCTCTCTTTATTTCATAATTTTCCGCGCGCGATA GGCCCTAGACCAGCGTTCTCGGTCGTTGAGGGTGCGGTGTATCTTCTCCAGGACGTGGTAGAGGTGGC TCTGGACGTTGAGATACATGGGCATGAGCCCGTCCCGGGGGTGGAGGTAGCACCACTGCAGAGCTTCA TGCTCCGGGGTGGTGTTGTAGATGATCCAGTCGTAGCAGGAGCGCTGGGCATGGTGCCTAAAAATGTC CTTCAGCAGCAGGCCGATGGCCAGGGGGAGGCCCTTGGTGTAAGTGTTTACAAAACGGTTAAGTTGGG AAGGGTGCATTCGGGGAGAGATGATGTGCATCTTGGACTGTATTTTTAGATTGGCGATGTTTCCGCCCA GATCCCTTCTGGGATTCATGTTGTGCAGGACCACCAGTACAGTGTATCCGGTGCACTTGGGGAATTTGT CATGCAGCTTAGAGGGAAAAGCGTGGAAGAACTTGGAGACGCCTTTGTGGCCTCCCAGATTTTCCATG CATTCGTCCATGATGATGGCAATGGGCCCGCGGGAGGCAGCTTGGGCAAAGATATTTCTGGGGTCGCT GACGTCGTAGTTGTGTTCCAGGGTGAGGTCGTCATAGGCCATTTTTACAAAGCGCGGGCGGAGGGTGC CCGACTGGGGGATGATGGTCCCCTCTGGCCCTGGGGCGTAGTTGCCCTCGCAGATCTGCATTTCCCAG

GCCTTAATCTCGGAGGGGGGAATCATATCCACCTGCGGGGCGATGAAGAAAACGGTTTCCGGAGCCG GGGAGATTAACTGGGATGAGAGCAGGTTTCTAAGCAGCTGTGATTTTCCACAACCGGTGGGCCCATAA ATAACACCTATAACCGGTTGCAGCTGGTAGTTTAGAGAGCTGCAGCTGCCGTCGTCCCGGAGGAGGGG GGCCACCTCGTTGAGCATGTCCCTGACGCGCATGTTCTCCCCGACCAGATCCGCCAGAAGGCGCTCGC CGCCCAGGGACAGCAGCTCTTGCAAGGAAGCAAAGTTTTTCAGCGGCTTGAGGCCGTCCGCCGTGGGC ATGTTTTTCAGGGTCTGGCTCAGCAGCTCCAGGCGGTCCCAGAGCTCGGTGACGTGCTCTACGGCATCT CTATCCAGCATATCTCCTCGTTTCGCGGGTTGGGGCGACTTTCGCTGTAGGGCACCAAGCGGTGGTCGT CCAGCGGGGCCAGAGTCATGTCCTTCCATGGGCGCAGGGTCCTCGTCAGGGTGGTCTGGGTCACGGTG AAGGGGTGCGCTCCGGGCTGAGCGCTTGCCAAGGTGCGCTTGAGGCTGGTTCTGCTGGTGCTGAAGCG CTGCCGGTCTTCGCCCTGCGCGTCGGCCAGGTAGCATTTGACCATGGTGTCATAGTCCAGCCCCTCCGC GGCGTGTCCCTTGGCGCGCAGCTTGCCCTTGGAGGTGGCGCCGCACGAGGGGCAGAGCAGGCTCTTGA GCGCGTAGAGCTTGGGGGCGAGGAAGACCGATTCGGGGGAGTAGGCGTCCGCGCCGCAGACCCCGCA CACGGTCTCGCACTCCACCAGCCAGGTGAGCTCGGGGCGCGCCGGGTCAAAAACCAGGTTTCCCCCAT GCTTTTTGATGCGTTTCTTACCTCGGGTCTCCATGAGGTGGTGTCCCCGCTCGGTGACGAAGAGGCTGT CCGTGTCTCCGTAGACCGACTTGAGGGGTCTTTTCTCCAGGGGGGTCCCTCGGTCTTCCTCGTAGAGGA ACTCGGACCACTCTGAGACGAAGGCCCGCGTCCAGGCCAGGACGAAGGAGGCTATGTGGGAGGGGTA GCGGTCGTTGTCCACTAGGGGGTCCACCTTCTCCAAGGTGTGAAGACACATGTCGCCTTCCTCGGCGTC CAGGAAGGTGATTGGCTTGTAGGTGTAGGCCACGTGACCGGGGGTTCCTGACGGGGGGGTATAAAAG GGGGTGGGGGCGCGCTCGTCGTCACTCTCTTCCGCATCGCTGTCTGCGAGGGCCAGCTGCTGGGGTGA GTATTCCCTCTCGAAGGCGGGCATGACCTCCGCGCTGAGGTTGTCAGTTTCCAAAAACGAGGAGGATT TGATGTTCACCTGTCCCGAGGTGATACCTTTGAGGGTACCCGCGTCCATCTGGTCAGAAAACACGATCT TTTTATTGTCCAGCTTGGTGGCGAACGACCCGTAGAGGGCGTTGGAGAGCAGCTTGGCGATGGAGCGC AGGGTCTGGTTCTTGTCCCTGTCGGCGCGCTCCTTGGCCGCGATGTTGAGCTGCACGTACTCGCGCGCG ACGCAGCGCCACTCGGGGAAGACGGTGGTGCGCTCGTCGGGCACCAGGCGCACGCGCCAGCCGCGGT TGTGCAGGGTGACCAGGTCCACGCTGGTGGCGACCTCGCCGCGCAGGCGCTCGTTGGTCCAGCAGAGA CGGCCGCCCTTGCGCGAGCAGAAGGGGGGCAGGGGGTCGAGCTGGGTCTCGTCCGGGGGGTCCGCGT CCACGGTGAAAACCCCGGGGCGCAGGCGCGCGTCGAAGTAGTCTATCTTGCAACCTTGCATGTCCAGC GCCTGCTGCCAGTCGCGGGCGGCGAGCGCGCGCTCGTAGGGGTTGAGCGGCGGGCCCCAGGGCATGG GGTGGGTGAGTGCGGAGGCGTACATGCCGCAGATGTCATAGACGTAGAGGGGCTCCCGCAGGACCCC GATGTAGGTGGGGTAGCAGCGGCCGCCGCGGATGCTGGCGCGCACGTAGTCATACAGCTCGTGCGAG GGGGCGAGGAGGTCGGGGCCCAGGTTGGTGCGGGCGGGGCGCTCCGCGCGGAAGACGATCTGCCTGA AGATGGCATGCGAGTTGGAAGAGATGGTGGGGCGCTGGAAGACGTTGAAGCTGGCGTCCTGCAGGCC GACGGCGTCGCGCACGAAGGAGGCGTAGGAGTCGCGCAGCTTGTGTACCAGCTCGGCGGTGACCTGC ACGTCGAGCGCGCAGTAGTCGAGGGTCTCGCGGATGATGTCATATTTAGCCTGCCCCTTCTTTTTCCAC AGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTACTCTTGGATCGGGAAACCGTCCGGTTCC GAACGGTAAGAGCCTAGCATGTAGAACTGGTTGACGGCCTGGTAGGCGCAGCAGCCCTTCTCCACGGG GAGGGCGTAGGCCTGCGCGGCCTTGCGGAGCGAGGTGTGGGTCAGGGCGAAGGTGTCCCTGACCATG ACTTTGAGGTACTGGTGCTTGAAGTCGGAGTCGTCGCAGCCGCCCCGCTCCCAGAGCGAGAAGTCGGT GCGCTTCTTGGAGCGGGGGTTGGGCAGAGCGAAGGTGACATCGTTGAAGAGGATTTTGCCCGCGCGG GGCATGAAGTTGCGGGTGATGCGGAAGGGCCCCGGCACTTCAGAGCGGTTGTTGATGACCTGGGCGG CGAGCACGATCTCGTCGAAGCCGTTGATGTTGTGGCCCACGATGTAGAGTTCCAGGAAGCGGGGCCGG CCCTTTACGGTGGGCAGCTTCTTTAGCTCTTCGTAGGTGAGCTCCTCGGGCGAGGCGAGGCCGTGCTCG GCCAGGGCCCAGTCCGCGAGGTGCGGGTTGTCTCTGAGGAAGGACTTCCAGAGGTCGCGGGCCAGGA GGGTCTGCAGGCGGTCTCTGAAGGTCCTGAACTGGCGGCCCACGGCCATTTTTTCGGGGGTGATGCAG TAGAAGGTGAGGGGGTCTTGCTGCCAGCGGTCCCAGTCGAGCTGCAGGGCGAGGTCGCGCGCGGCGG TGACCAGGCGCTCGTCGCCCCCGAATTTCATGACCAGCATGAAGGGCACGAGCTGCTTTCCGAAGGCC CCCATCCAAGTGTAGGTCTCTACATCGTAGGTGACAAAGAGGCGCTCCGTGCGAGGATGCGAGCCGAT CGGGAAGAACTGGATCTCCCGCCACCAGTTGGAGGAGTGGCTGTTGATGTGGTGGAAGTAGAAGTCCC GTCGCCGGGCCGAACACTCGTGCTGGCTTTTGTAAAAGCGAGCGCAGTACTGGCAGCGCTGCACGGGC TGTACCTCATGCACGAGATGCACCTTTCGCCCGCGCACGAGGAAGCCGAGGGGAAATCTGAGCCCCCC GCCTGGCTCGCGGCATGGCTGGTTCTCTTCTACTTTGGATGCGTGTCCGTCTCCGTCTGGCTCCTCGAG GGGTGTTACGGTGGAGCGGACCACCACGCCGCGCGAGCCGCAGGTCCAGATATCGGCGCGCGGCGGT CGGAGTTTGATGACGACATCGCGCAGCTGGGAGCTGTCCATGGTCTGGAGCTCCCGCGGCGGCGGCAG GTCAGCCGGGAGTTCTTGCAGGTTCACCTCGCAGAGTCGGGCCAGGGCGCGGGGCAGGTCTAGGTGGT ACCTGATCTCTAGGGGCGTGTTGGTGGCGGCGTCGATGGCTTGCAGGAGCCCGCAGCCCCGGGGGGCG ACGACGGTGCCCCGCGGGGTGGTGGTGGTGGTGGCGGTGCAGCTCAGAAGCGGTGCCGCGGGCGGGC CCCCGGAGGTAGGGGGGGCTCCGGTCCCGCGGGCAGGGGCGGCAGCGGCACGTCGGCGTGGAGCGCG GGCAGGAGTTGGTGCTGTGCCCGGAGGTTGCTGGCGAAGGCGACGACGCGGCGGTTGATCTCCTGGAT CTGGCGCCTCTGCGTGAAGACGACGGGCCCGGTGAGCTTGAACCTGAAAGAGAGTTCGACAGAATCA ATCTCGGTGTCATTGACCGCGGCCTGGCGCAGGATCTCCTGCACGTCTCCCGAGTTGTCTTGGTAGGCG ATCTCGGCCATGAACTGCTCGATCTCTTCCTCCTGGAGGTCTCCGCGTCCGGCGCGTTCCACGGTGGCC GCCAGGTCGTTGGAGATGCGCCCCATGAGCTGCGAGAAGGCGTTGAGTCCGCCCTCGTTCCAGACTCG GCTGTAGACCACGCCCCCCTGGTCATCGCGGGCGCGCATGACCACCTGCGCGAGGTTGAGCTCCACGT GCCGCGCGAAGACGGCGTAGTTGCGCAGACGCTGGAAGAGGTAGTTGAGGGTGGTGGCGGTGTGCTC GGCCACGAAGAAGTTCATGACCCAGCGGCGCAACGTGGATTCGTTGATGTCCCCCAAGGCCTCCAGCC GTTCCATGGCCTCGTAGAAGTCCACGGCGAAGTTGAAAAACTGGGAGTTGCGCGCCGACACGGTCAAC TCCTCCTCCAGAAGACGGATGAGCTCGGCGACGGTGTCGCGCACCTCGCGCTCGAAGGCTATGGGGAT CTCTTCCTCCGCTAGCATCACCACCTCCTCCTCTTCCTCCTCTTCTGGCACTTCCATGATGGCTTCCTCCT CTTCGGGGGGTGGCGGCGGCGGCGGTGGGGGAGGGGGCGCTCTGCGCCGGCGGCGGCGCACCGGGAG GCGGTCCACGAAGCGCGCGATCATCTCCCCGCGGCGGCGGCGCATGGTCTCGGTGACGGCGCGGCCGT TCTCCCGGGGGCGCAGTTGGAAGACGCCGCCGGACATCTGGTGCTGGGGCGGGTGGCCGTGAGGCAG CGAGACGGCGCTGACGATGCATCTCAACAATTGCTGCGTAGGTACGCCGCCGAGGGACCTGAGGGAG TCCATATCCACCGGATCCGAAAACCTTTCGAGGAAGGCGTCTAACCAGTCGCAGTCGCAAGGTAGGCT GAGCACCGTGGCGGGCGGCGGGGGGTGGGGGGAGTGTCTGGCGGAGGTGCTGCTGATGATGTAATTG AAGTAGGCGGACTTGACACGGCGGATGGTCGACAGGAGCACCATGTCCTTGGGTCCGGCCTGCTGGAT GCGGAGGCGGTCGGCTATGCCCCAGGCTTCGTTCTGGCATCGGCGCAGGTCCTTGTAGTAGTCTTGCAT GAGCCTTTCCACCGGCACCTCTTCTCCTTCCTCTTCTGCTTCTTCCATGTCTGCTTCGGCCCTGGGGCGG CGCCGCGCCCCCCTGCCCCCCATGCGCGTGACCCCGAACCCCCTGAGCGGTTGGAGCAGGGCCAGGTC GGCGACGACGCGCTCGGCCAGGATGGCCTGCTGCACCTGCGTGAGGGTGGTTTGGAAGTCATCCAAGT CCACGAAGCGGTGGTAGGCGCCCGTGTTGATGGTGTAGGTGCAGTTGGCCATGACGGACCAGTTGACG GTCTGGTGGCCCGGTTGCGACATCTCGGTGTACCTGAGTCGCGAGTAGGCGCGGGAGTCGAAGACGTA GTCGTTGCAAGTCCGCACCAGGTACTGGTAGCCCACCAGGAAGTGCGGCGGCGGCTGGCGGTAGAGG GGCCAGCGCAGGGTGGCGGGGGCTCCGGGGGCCAGGTCTTCCAGCATGAGGCGGTGGTAGGCGTAGA TGTACCTGGACATCCAGGTGATACCCGCGGCGGTGGTGGAGGCGCGCGGGAAGTCGCGCACCCGGTTC CAGATGTTGCGCAGGGGCAGAAAGTGCTCCATGGTAGGCGTGCTCTGTCCAGTCAGACGCGCGCAGTC GTTGATACTCTAGACCAGGGAAAACGAAAGCCGGTCAGCGGGCACTCTTCCGTGGTCTGGTGAATAGA TCGCAAGGGTATCATGGCGGAGGGCCTCGGTTCGAGCCCCGGGTCCGGGCCGGACGGTCCGCCATGAT CCACGCGGTTACCGCCCGCGTGTCGAACCCAGGTGTGCGACGTCAGACAACGGTGGAGTGTTCCTTTT GGCGTTTTTCTGGCCGGGCGCCGGCGCCGCGTAAGAGACTAAGCCGCGAAAGCGAAAGCAGTAAGTG GCTCGCTCCCCGTAGCCGGAGGGATCCTTGCTAAGGGTTGCGTTGCGGCGAACCCCGGTTCGAATCCC GTACTCGGGCCGGCCGGACCCGCGGCTAAGGTGTTGGATTGGCCTCCCCCTCGTATAAAGACCCCGCT TGCGGATTGACTCCGGACACGGGGACGAGCCCCTTTTATTTTTGCTTTCCCCAGATGCATCCGGTGCTG CGGCAGATGCGCCCCCCGCCCCAGCAGCAGCAACAACACCAGCAAGAGCGGCAGCAACAGCAGCGGG AGTCATGCAGGGCCCCCTCACCCACCCTCGGCGGGCCGGCCACCTCGGCGTCCGCGGCCGTGTCTGGC GCCTGCGGCGGCGGCGGGGGGCCGGCTGACGACCCCGAGGAGCCCCCGCGGCGCAGGGCCAGACACT ACCTGGACCTGGAGGAGGGCGAGGGCCTGGCGCGGCTGGGGGCGCCGTCTCCCGAGCGCCACCCGCG GGTGCAGCTGAAGCGCGACTCGCGCGAGGCGTACGTGCCTCGGCAGAACCTGTTCAGGGACCGCGCG GGCGAGGAGCCCGAGGAGATGCGGGACAGGAGGTTCAGCGCAGGGCGGGAGCTGCGGCAGGGGCTG AACCGCGAGCGGCTGCTGCGCGAGGAGGACTTTGAGCCCGACGCGCGGACGGGGATCAGCCCCGCGC GCGCGCACGTGGCGGCCGCCGACCTGGTGACGGCGTACGAGCAGACGGTGAACCAGGAGATCAACTT CCAAAAGAGTTTCAACAACCACGTGCGCACGCTGGTGGCGCGCGAGGAGGTGACCATCGGGCTGATG CACCTGTGGGACTTTGTAAGCGCGCTGGTGCAGAACCCCAACAGCAAGCCTCTGACGGCGCAGCTGTT CCTGATAGTGCAGCACAGCAGGGACAACGAGGCGTTTAGGGACGCGCTGCTGAACATCACCGAGCCC GAGGGTCGGTGGCTGCTGGACCTGATTAACATCCTGCAGAGCATAGTGGTGCAGGAGCGCAGCCTGA GCCTGGCCGACAAGGTGGCGGCCATCAACTACTCGATGCTGAGCCTGGGCAAGTTTTACGCGCGCAAG ATCTACCAGACGCCGTACGTGCCCATAGACAAGGAGGTGAAGATCGACGGTTTTTACATGCGCATGGC GCTGAAGGTGCTCACCCTGAGCGACGACCTGGGCGTGTACCGCAACGAGCGCATCCACAAGGCCGTG AGCGTGAGCCGGCGGCGCGAGCTGAGCGACCGCGAGCTGATGCACAGCCTGCAGCGGGCGCTGGCGG GCGCCGGCAGCGGCGACAGGGAGGCGGAGTCCTACTTCGATGCGGGGGCGGACCTGCGCTGGGCGCC CAGCCGGCGGGCCCTGGAGGCCGCGGGGGTCCGCGAGGACTATGACGAGGACGGCGAGGAGGATGA GGAGTACGAGCTAGAGGAGGGCGAGTACCTGGACTAAACCGCGGGTGGTGTTTCCGGTAGATGCAAG ACCCGAACGTGGTGGACCCGGCGCTGCGGGCGGCTCTGCAGAGCCAGCCGTCCGGCCTTAACTCCTCA GACGACTGGCGACAGGTCATGGACCGCATCATGTCGCTGACGGCGCGTAACCCGGACGCGTTCCGGCA GCAGCCGCAGGCCAACAGGCTCTCCGCCATCCTGGAGGCGGTGGTGCCTGCGCGCTCGAACCCCACGC ACGAGAAGGTGCTGGCCATAGTGAACGCGCTGGCCGAGAACAGGGCCATCCGCCCGGACGAGGCCGG GCTGGTGTACGACGCGCTGCTGCAGCGCGTGGCCCGCTACAACAGCGGCAACGTGCAGACCAACCTG GACCGGCTGGTGGGGGACGTGCGCGAGGCGGTGGCGCAGCGCGAGCGCGCGGATCGGCAGGGCAACC TGGGCTCCATGGTGGCGCTGAATGCCTTCCTGAGCACGCAGCCGGCCAACGTGCCGCGGGGGCAGGA AGACTACACCAACTTTGTGAGCGCGCTGCGGCTGATGGTGACCGAGACCCCCCAGAGCGAGGTGTACC AGTCGGGCCCGGACTACTTCTTCCAGACCAGCAGACAGGGCCTGCAGACGGTGAACCTGAGCCAGGCT TTCAAGAACCTGCGGGGGCTGTGGGGCGTGAAGGCGCCCACCGGCGACCGGGCGACGGTGTCCAGCC TGCTGACGCCCAACTCGCGCCTGCTGCTGCTGCTGATCGCGCCGTTCACGGACAGCGGCAGCGTGTCC CGGGACACCTACCTGGGGCACCTGCTGACCCTGTACCGCGAGGCCATCGGGCAGGCGCAGGTGGACG AGCACACCTTCCAGGAGATCACCAGCGTGAGCCGCGCGCTGGGGCAGGAGGACACGAGCAGCCTGGA GGCGACTCTGAACTACCTGCTGACCAACCGGCGGCAGAAGATTCCCTCGCTGCACAGCCTGACCTCCG

AGGAGGAGCGCATCTTGCGCTACGTGCAGCAGAGCGTGAGCCTGAACCTGATGCGCGACGGGGTGAC GCCCAGCGTGGCGCTGGACATGACCGCGCGCAACATGGAACCGGGCATGTACGCCGCGCACCGGCCT TACATCAACCGCCTGATGGACTACCTGCATCGCGCGGCGGCCGTGAACCCCGAGTACTTTACCAACGC CATCCTGAACCCGCACTGGCTCCCGCCGCCCGGGTTCTACAGCGGGGGCTTCGAGGTCCCGGAGACCA ACGATGGCTTCCTGTGGGACGACATGGACGACAGCGTGTTCTCCCCGCGGCCGCAGGCGCTGGCGGAA GCGTCCCTGCTGCGTCCCAAGAAGGAGGAGGAGGAGGAGGCGAGTCGCCGCCGCGGCAGCAGCGGCG TGGCTTCTCTGTCCGAGCTGGGGGCGGCAGCCGCCGCGCGCCCCGGGTCCCTGGGCGGCAGCCCCTTT CCGAGCCTGGTGGGGTCTCTGCACAGCGAGCGCACCACCCGCCCTCGGCTGCTGGGCGAGGACGAGTA CCTGAATAACTCCCTGCTGCAGCCGGTGCGGGAGAAAAACCTGCCTCCCGCCTTCCCCAACAACGGGA TAGAGAGCCTGGTGGACAAGATGAGCAGATGGAAGACCTATGCGCAGGAGCACAGGGACGCGCCTGC GCTCCGGCCGCCCACGCGGCGCCAGCGCCACGACCGGCAGCGGGGGCTGGTGTGGGATGACGAGGAC TCCGCGGACGATAGCAGCGTGCTGGACCTGGGAGGGAGCGGCAACCCGTTCGCGCACCTGCGCCCCC GCCTGGGGAGGATGTTTTAAAAAAAAAAAAAAAAAGCAAGAAGCATGATGCAAAAATTAAATAAAA CTCACCAAGGCCATGGCGACCGAGCGTTGGTTTCTTGTGTTCCCTTCAGTATGCGGCGCGCGGCGATGT ACCAGGAGGGACCTCCTCCCTCTTACGAGAGCGTGGTGGGCGCGGCGGCGGCGGCGCCCTCTTCTCCC TTTGCGTCGCAGCTGCTGGAGCCGCCGTACGTGCCTCCGCGCTACCTGCGGCCTACGGGGGGGAGAAA CAGCATCCGTTACTCGGAGCTGGCGCCCCTGTTCGACACCACCCGGGTGTACCTGGTGGACAACAAGT CGGCGGACGTGGCCTCCCTGAACTACCAGAACGACCACAGCAATTTTTTGACCACGGTCATCCAGAAC AATGACTACAGCCCGAGCGAGGCCAGCACCCAGACCATCAATCTGGATGACCGGTCGCACTGGGGCG GCGACCTGAAAACCATCCTGCACACCAACATGCCCAACGTGAACGAGTTCATGTTCACCAATAAGTTC AAGGCGCGGGTGATGGTGTCGCGCTCGCACACCAAGGAAGACCGGGTGGAGCTGAAGTACGAGTGGG TGGAGTTCGAGCTGCCAGAGGGCAACTACTCCGAGACCATGACCATTGACCTGATGAACAACGCGATC GTGGAGCACTATCTGAAAGTGGGCAGGCAGAACGGGGTCCTGGAGAGCGACATCGGGGTCAAGTTCG ACACCAGGAACTTCCGCCTGGGGCTGGACCCCGTGACCGGGCTGGTTATGCCCGGGGTGTACACCAAC GAGGCCTTCCATCCCGACATCATCCTGCTGCCCGGCTGCGGGGTGGACTTCACTTACAGCCGCCTGAG CAACCTCCTGGGCATCCGCAAGCGGCAGCCCTTCCAGGAGGGCTTCAGGATCACCTACGAGGACCTGG AGGGGGGCAACATCCCCGCGCTCCTCGATGTGGAGGCCTACCAGGATAGCTTGAAGGAAAATGAGGC GGGACAGGAGGATACCGCCCCCGCCGCCTCCGCCGCCGCCGAGCAGGGCGAGGATGCTGCTGACACC GCGGCCGCGGACGGGGCAGAGGCCGACCCCGCTATGGTGGTGGAGGCTCCCGAGCAGGAGGAGGACA TGAATGACAGTGCGGTGCGCGGAGACACCTTCGTCACCCGGGGGGAGGAAAAGCAAGCGGAGGCCGA GGCCGCGGCCGAGGAAAAGCAACTGGCGGCAGCAGCGGCGGCGGCGGCGTTGGCCGCGGCGGAGGC TGAGTCTGAGGGGACCAAGCCCGCCAAGGAGCCCGTGATTAAGCCCCTGACCGAAGATAGCAAGAAG CGCAGTTACAACCTGCTCAAGGACAGCACCAACACCGCGTACCGCAGCTGGTACCTGGCCTACAACTA CGGCGACCCGTCGACGGGGGTGCGCTCCTGGACCCTGCTGTGCACGCCGGACGTGACCTGCGGCTCGG AGCAGGTGTACTGGTCGCTGCCCGACATGATGCAAGACCCCGTGACCTTCCGCTCCACGCGGCAGGTC AGCAACTTCCCGGTGGTGGGCGCCGAGCTGCTGCCCGTGCACTCCAAGAGCTTCTACAACGACCAGGC CGTCTACTCCCAGCTCATCCGCCAGTTCACCTCTCTGACCCACGTGTTCAATCGCTTTCCTGAGAACCA GATTCTGGCGCGCCCGCCCGCCCCCACCATCACCACCGTCAGTGAAAACGTTCCTGCTCTCACAGATC ACGGGACGCTACCGCTGCGCAACAGCATCGGAGGAGTCCAGCGAGTGACCGTTACTGACGCCAGACG CCGCACCTGCCCCTACGTTTACAAGGCCTTGGGCATAGTCTCGCCGCGCGTCCTTTCCAGCCGCACTTT TTGAGCAACACCACCATCATGTCCATCCTGATCTCACCCAGCAATAACTCCGGCTGGGGACTGCTGCG CGCGCCCAGCAAGATGTTCGGAGGGGCGAGGAAGCGTTCCGAGCAGCACCCCGTGCGCGTGCGCGGG CACTTCCGCGCCCCCTGGGGAGCGCACAAACGCGGCCGCGCGGGGCGCACCACCGTGGACGACGCCA TCGACTCGGTGGTGGAGCAGGCGCGCAACTACAGGCCCGCGGTCTCTACCGTGGACGCGGCCATCCAG ACCGTGGTGCGGGGCGCGCGGCGGTACGCCAAGCTGAAGAGCCGCCGGAAGCGCGTGGCCCGCCGCC ACCGCCGCCGACCCGGGGCCGCCGCCAAACGCGCCGCCGCGGCCCTGCTTCGCCGGGCCAAGCGCAC GGGCCGCCGCGCCGCCATGAGGGCCGCGCGCCGCTTGGCCGCCGGCATCACCGCCGCCACCATGGCCC CCCGTACCCGAAGACGCGCGGCCGCCGCCGCCGCCGCCGCCATCAGTGACATGGCCAGCAGGCGCCG GGGCAACGTGTACTGGGTGCGCGACTCGGTGACCGGCACGCGCGTGCCCGTGCGCTTCCGCCCCCCGC GGACTTGAGATGATGTGAAAAAACAACACTGAGTCTCCTGCTGTTGTGTGTATCCCAGCGGCGGCGGC GCGCGCAGCGTCATGTCCAAGCGCAAAATCAAAGAAGAGATGCTCCAGGTCGTCGCGCCGGAGATCT ATGGGCCCCCGAAGAAGGAAGAGCAGGATTCGAAGCCCCGCAAGATAAAGCGGGTCAAAAAGAAAA AGAAAGATGATGACGATGCCGATGGGGAGGTGGAGTTCCTGCGCGCCACGGCGCCCAGGCGCCCGGT GCAGTGGAAGGGCCGGCGCGTAAAGCGCGTCCTGCGCCCCGGCACCGCGGTGGTCTTCACGCCCGGC GAGCGCTCCACCCGGACTTTCAAGCGCGTCTATGACGAGGTGTACGGCGACGAAGACCTGCTGGAGCA GGCCAACGAGCGCTTCGGAGAGTTTGCTTACGGGAAGCGTCAGCGGGCGCTGGGGAAGGAGGACCTG CTGGCGCTGCCGCTGGACCAGGGCAACCCCACCCCCAGTCTGAAGCCCGTGACCCTGCAGCAGGTGCT GCCGAGCAGCGCACCCTCCGAGGCGAAGCGGGGTCTGAAGCGCGAGGGCGGCGACCTGGCGCCCACC GTGCAGCTCATGGTGCCCAAGCGGCAGAGGCTGGAGGATGTGCTGGAGAAAATGAAAGTAGACCCCG GTCTGCAGCCGGACATCAGGGTCCGCCCCATCAAGCAGGTGGCGCCGGGCCTCGGCGTGCAGACCGTG GACGTGGTCATCCCCACCGGCAACTCCCCCGCCGCCGCCACCACTACCGCTGCCTCCACGGACATGGA GACACAGACCGATCCCGCCGCAGCCGCAGCCGCAGCCGCCGCCGCGACCTCCTCGGCGGAGGTGCAG ACGGACCCCTGGCTGCCGCCGGCGATGTCAGCTCCCCGCGCGCGTCGCGGGCGCAGGAAGTACGGCG CCGCCAACGCGCTCCTGCCCGAGTACGCCTTGCATCCTTCCATCGCGCCCACCCCCGGCTACCGAGGCT ATACCTACCGCCCGCGAAGAGCCAAGGGTTCCACCCGCCGTCCCCGCCGACGCGCCGCCGCCACCACC CGCCGCCGCCGCCGCAGACGCCAGCCCGCACTGGCTCCAGTCTCCGTGAGGAAAGTGGCGCGCGACG GACACACCCTGGTGCTGCCCAGGGCGCGCTACCACCCCAGCATCGTTTAAAAGCCTGTTGTGGTTCTTG CAGATATGGCCCTCACTTGCCGCCTCCGTTTCCCGGTGCCGGGATACCGAGGAGGAAGATCGCGCCGC AGGAGGGGTCTGGCCGGCCGCGGCCTGAGCGGAGGCAGCCGCCGCGCGCACCGGCGGCGACGCGCCA CCAGCCGACGCATGCGCGGCGGGGTGCTGCCCCTGTTAATCCCCCTGATCGCCGCGGCGATCGGCGCC GTGCCCGGGATCGCCTCCGTGGCCTTGCAAGCGTCCCAGAGGCATTGACAGACTTGCAAACTTGCAAA TATGGAAAAAAAAACCCCAATAAAAAAGTCTAGACTCTCACGCTCGCTTGGTCCTGTGACTATTTTGT AGAATGGAAGACATCAACTTTGCGTCGCTGGCCCCGCGTCACGGCTCGCGCCCGTTCCTGGGACACTG GAACGATATCGGCACCAGCAACATGAGCGGTGGCGCCTTCAGTTGGGGCTCTCTGTGGAGCGGCATTA AAAGTATCGGGTCTGCCGTTAAAAATTACGGCTCCCGGGCCTGGAACAGCAGCACGGGCCAGATGTTG AGAGACAAGTTGAAAGAGCAGAACTTCCAGCAGAAGGTGGTGGAGGGCCTGGCCTCCGGCATCAACG GGGTGGTGGACCTGGCCAACCAGGCCGTGCAGAATAAGATCAACAGCAGACTGGACCCCCGGCCGCC GGTGGAGGAGGTGCCGCCGGCGCTGGAGACGGTGTCCCCCGATGGGCGTGGCGAGAAGCGCCCGCGG CCCGATAGGGAAGAGACCACTCTGGTCACGCAGACCGATGAGCCGCCCCCGTATGAGGAGGCCCTGA AGCAAGGTCTGCCCACCACGCGGCCCATCGCGCCCATGGCCACCGGGGTGGTGGGCCGCCACACCCCC GCCACGCTGGACTTGCCTCCGCCCGCCGATGTGCCGCAGCAGCAGAAGGCGGCACAGCCGGGCCCGC CCGCGACCGCCTCCCGTTCCTCCGCCGGTCCTCTGCGCCGCGCGGCCAGCGGCCCCCGCGGGGGGGTC GCGAGGCACGGCAACTGGCAGAGCACGCTGAACAGCATCGTGGGTCTGGGGGTGCGGTCCGTGAAGC GCCGCCGATGCTACTGAATAGCTTAGCTAACGTGTTGTATGTGTGTATGCGCCCTATGTCGCCGCCAGA GGAGCTGCTGAGTCGCCGCCGTTCGCGCGCCCACCACCACCGCCACTCCGCCCCTCAAGATGGCGACC CCATCGATGATGCCGCAGTGGTCGTACATGCACATCTCGGGCCAGGACGCCTCGGAGTACCTGAGCCC CGGGCTGGTGCAGTTCGCCCGCGCCACCGAGAGCTACTTCAGCCTGAGTAACAAGTTTAGGAACCCCA CGGTGGCGCCCACGCACGATGTGACCACCGACCGGTCTCAGCGCCTGACGCTGCGGTTCATTCCCGTG GACCGCGAGGACACCGCGTACTCGTACAAGGCGCGGTTCACCCTGGCCGTGGGCGACAACCGCGTGCT GGACATGGCCTCCACCTACTTTGACATCCGCGGGGTGCTGGACCGGGGTCCCACTTTCAAGCCCTACTC TGGCACCGCCTACAACTCCCTGGCCCCCAAGGGCGCTCCCAACTCCTGCGAGTGGGAGCAAGAGGAA ACTCAGGCAGTTGAAGAAGCAGCAGAAGAGGAAGAAGAAGATGCTGACGGTCAAGCTGAGGAAGAG CAAGCAGCTACCAAAAAGACTCATGTATATGCTCAGGCTCCCCTTTCTGGCGAAAAAATTAGTAAAGA TGGTCTGCAAATAGGAACGGACGCTACAGCTACAGAACAAAAACCTATTTATGCAGACCCTACATTCC AGCCCGAACCCCAAATCGGGGAGTCCCAGTGGAATGAGGCAGATGCTACAGTCGCCGGCGGTAGAGT GCTAAAGAAATCTACTCCCATGAAACCATGCTATGGTTCCTATGCAAGACCCACAAATGCTAATGGAG GTCAGGGTGTACTAACGGCAAATGCCCAGGGACAGCTAGAATCTCAGGTTGAAATGCAATTCTTTTCA ACTTCTGAAAACGCCCGTAACGAGGCTAACAACATTCAGCCCAAATTGGTGCTGTATAGTGAGGATGT GCACATGGAGACCCCGGATACGCACCTTTCTTACAAGCCCGCAAAAAGCGATGACAATTCAAAAATCA TGCTGGGTCAGCAGTCCATGCCCAACAGACCTAATTACATCGGCTTCAGAGACAACTTTATCGGCCTC ATGTATTACAATAGCACTGGCAACATGGGAGTGCTTGCAGGTCAGGCCTCTCAGTTGAATGCAGTGGT GGACTTGCAAGACAGAAACACAGAACTGTCCTACCAGCTCTTGCTTGATTCCATGGGTGACAGAACCA GATACTTTTCCATGTGGAATCAGGCAGTGGACAGTTATGACCCAGATGTTAGAATTATTGAAAATCAT GGAACTGAAGACGAGCTCCCCAACTATTGTTTCCCTCTGGGTGGCATAGGGGTAACTGACACTTACCA GGCTGTTAAAACCAACAATGGCAATAACGGGGGCCAGGTGACTTGGACAAAAGATGAAACTTTTGCA GATCGCAATGAAATAGGGGTGGGAAACAATTTCGCTATGGAGATCAACCTCAGTGCCAACCTGTGGA GAAACTTCCTGTACTCCAACGTGGCGCTGTACCTACCAGACAAGCTTAAGTACAACCCCTCCAATGTG GACATCTCTGACAACCCCAACACCTACGATTACATGAACAAGCGAGTGGTGGCCCCGGGGCTGGTGGA CTGCTACATCAACCTGGGCGCGCGCTGGTCGCTGGACTACATGGACAACGTCAACCCCTTCAACCACC ACCGCAATGCGGGCCTGCGCTACCGCTCCATGCTCCTGGGCAACGGGCGCTACGTGCCCTTCCACATC CAGGTGCCCCAGAAGTTCTTTGCCATCAAGAACCTCCTCCTCCTGCCGGGCTCCTACACCTACGAGTGG AACTTCAGGAAGGATGTCAACATGGTCCTCCAGAGCTCTCTGGGTAACGATCTCAGGGTGGACGGGGC CAGCATCAAGTTCGAGAGCATCTGCCTCTACGCCACCTTCTTCCCCATGGCCCACAACACGGCCTCCAC GCTCGAGGCCATGCTCAGGAACGACACCAACGACCAGTCCTTCAATGACTACCTCTCCGCCGCCAACA TGCTCTACCCCATACCCGCCAACGCCACCAACGTCCCCATCTCCATCCCCTCGCGCAACTGGGCGGCCT TCCGCGGCTGGGCCTTCACCCGCCTCAAGACCAAGGAGACCCCCTCCCTGGGCTCGGGATTCGACCCC TACTACACCTACTCGGGCTCCATTCCCTACCTGGACGGCACCTTCTACCTCAACCACACTTTCAAGAAG GTCTCGGTCACCTTCGACTCCTCGGTCAGCTGGCCGGGCAACGACCGTCTGCTCACCCCCAACGAGTTC GAGATCAAGCGCTCGGTCGACGGGGAGGGCTACAACGTGGCCCAGTGCAACATGACCAAGGACTGGT TCCTGGTCCAGATGCTGGCCAACTACAACATCGGCTACCAGGGCTTCTACATCCCAGAGAGCTACAAG GACAGGATGTACTCCTTCTTCAGGAACTTCCAGCCCATGAGCCGGCAGGTGGTGGACCAGACCAAGTA CAAGGACTACCAGGAGGTGGGCATCATCCACCAGCACAACAACTCGGGCTTCGTGGGCTACCTCGCCC CCACCATGCGCGAGGGACAGGCCTACCCCGCCAACTTCCCCTATCCGCTCATAGGCAAGACCGCGGTC GACAGCATCACCCAGAAAAAGTTCCTCTGCGACCGCACCCTCTGGCGCATCCCCTTCTCCAGCAACTTC ATGTCCATGGGTGCGCTCTCGGACCTGGGCCAGAACTTGCTCTACGCCAACTCCGCCCACGCCCTCGA CATGACCTTCGAGGTCGACCCCATGGACGAGCCCACCCTTCTCTATGTTCTGTTCGAAGTCTTTGACGT

GGTCCGGGTCCACCAGCCGCACCGCGGCGTCATCGAGACCGTGTACCTGCGTACGCCCTTCTCGGCCG GCAACGCCACCACCTAAAGAAGCAAGCCGCAGTCATCGCCGCCTGCATGCCGTCGGGTTCCACCGAGC AAGAGCTCAGGGCCATCGTCAGAGACCTGGGATGCGGGCCCTATTTTTTGGGCACCTTCGACAAGCGC TTCCCTGGCTTTGTCTCCCCACACAAGCTGGCCTGCGCCATCGTCAACACGGCCGGCCGCGAGACCGG GGGCGTGCACTGGCTGGCCTTCGCCTGGAACCCGCGCTCCAAAACATGCTTCCTCTTTGACCCCTTCGG CTTTTCGGACCAGCGGCTCAAGCAAATCTACGAGTTCGAGTACGAGGGCTTGCTGCGTCGCAGCGCCA TCGCCTCCTCGCCCGACCGCTGCGTCACCCTCGAAAAGTCCACCCAGACCGTGCAGGGGCCCGACTCG GCCGCCTGCGGTCTCTTCTGCTGCATGTTTCTGCACGCCTTTGTGCACTGGCCTCAGAGTCCCATGGAC CGCAACCCCACCATGAACTTGCTGACGGGGGTGCCCAACTCCATGCTCCAGAGCCCCCAGGTCGAGCC CACCCTGCGCCGCAACCAGGAGCAGCTCTACAGCTTCCTGGAGCGCCACTCGCCTTACTTCCGCCGCC ACAGCGCACAGATCAGGAGGGCCACCTCCTTCTGCCACTTGCAAGAGATGCAAGAAGGGTAATAACG ATGTACACACTTTTTTTCTCAATAAATGGCATCTTTTTATTTATACAAGCTCTCTGGGGTATTCATTTCC CACCACCACCCGCCGTTGTCGCCATCTGGCTCTATTTAGAAATCGAAAGGGTTCTGCCGGGAGTCGCC GTGCGCCACGGGCAGGGACACGTTGCGATACTGGTAGCGGGTGCCCCACTTGAACTCGGGCACCACCA GGCGAGGCAGCTCGGGGAAGTTTTCGCTCCACAGGCTGCGGGTCAGCACCAGCGCGTTCATCAGGTCG GGCGCCGAGATCTTGAAGTCGCAGTTGGGGCCGCCGCCCTGCGCGCGCGAGTTGCGGTACACCGGGTT GCAGCACTGGAACACCAACAGCGCCGGGTGCTTCACGCTGGCCAGCACGCTGCGGTCGGAGATCAGC TCGGCGTCCAGGTCCTCCGCGTTGCTCAGCGCGAACGGGGTCATCTTGGGCACTTGCCGCCCCAGGAA GGGCGCGTGCCCCGGTTTCGAGTTGCAGTCGCAGCGCAGCGGGATCAGCAGGTGCCCGTGCCCGGACT CGGCGTTGGGGTACAGCGCGCGCATGAAGGCCTGCATCTGGCGGAAGGCCATCTGGGCCTTGGCGCCC TCCGAGAAGAACATGCCGCAGGACTTGCCCGAGAACTGGTTTGCGGGGCAGCTGGCGTCGTGCAGGC AGCAGCGCGCGTCGGTGTTGGCGATCTGCACCACGTTGCGCCCCCACCGGTTCTTCACGATCTTGGCCT TGGACGATTGCTCCTTCAGCGCGCGCTGCCCGTTCTCGCTGGTCACATCCATCTCGATCACATGTTCCT TGTTCACCATGCTGCTGCCGTGCAGACACTTCAGCTCGCCCTCCGTCTCGGTGCAGCGGTGCTGCCACA GCGCGCAGCCCGTGGGCTCGAAAGACTTGTAGGTCACCTCCGCGAAGGACTGCAGGTACCCCTGCAAA AAGCGGCCCATCATGGTCACGAAGGTCTTGTTGCTGCTGAAGGTCAGCTGCAGCCCGCGGTGCTCCTC GTTCAGCCAGGTCTTGCACACGGCCGCCAGCGCCTCCACCTGGTCGGGCAGCATCTTGAAGTTCACCTT CAGCTCATTCTCCACGTGGTACTTGTCCATCAGCGTGCGCGCCGCCTCCATGCCCTTCTCCCAGGCCGA CACCAGCGGCAGGCTCACGGGGTTCTTCACCATCACCGTGGCCGCCGCCTCCGCCGCGCTTTCGCTTTC CGCCCCGCTGTTCTCTTCCTCTTCCTCCTCTTCCTCGCCGCCGCCCACTCGCAGCCCCCGCACCACGGGG TCGTCTTCCTGCAGGCGCTGCACCTTGCGCTTGCCGTTGCGCCCCTGCTTGATGCGCACGGGCGGGTTG CTGAAGCCCACCATCACCAGCGCGGCCTCTTCTTGCTCGTCCTCGCTGTCCAGAATGACCTCCGGGGAG GGGGGGTTGGTCATCCTCAGTACCGAGGCACGCTTCTTTTTCTTCCTGGGGGCGTTCGCCAGCTCCGCG GCTGCGGCCGCTGCCGAGGTCGAAGGCCGAGGGCTGGGCGTGCGCGGCACCAGCGCGTCCTGCGAGC CGTCCTCGTCCTCCTCGGACTCGAGACGGAGGCGGGCCCGCTTCTTCGGGGGCGCGCGGGGCGGCGGA GGCGGCGGCGGCGACGGAGACGGGGACGAGACATCGTCCAGGGTGGGTGGACGGCGGGCCGCGCCG CGTCCGCGCTCGGGGGTGGTCTCGCGCTGGTCCTCTTCCCGACTGGCCATCTCCCACTGCTCCTTCTCCT ATAGGCAGAAAGAGATCATGGAGTCTCTCATGCGAGTCGAGAAGGAGGAGGACAGCCTAACCGCCCC CTCTGAGCCCTCCACCACCGCCGCCACCACCGCCAATGCCGCCGCGGACGACGCGCCCACCGAGACCA CCGCCAGTACCACCCTCCCCAGCGACGCACCCCCGCTCGAGAATGAAGTGCTGATCGAGCAGGACCCG GGTTTTGTGAGCGGAGAGGAGGATGAGGTGGATGAGAAGGAGAAGGAGGAGGTCGCCGCCTCAGTGC CAAAAGAGGATAAAAAGCAAGACCAGGACGACGCAGATAAGGATGAGACAGCAGTCGGGCGGGGGA ACGGAAGCCATGATGCTGATGACGGCTACCTAGACGTGGGAGACGACGTGCTGCTTAAGCACCTGCAC CGCCAGTGCGTCATCGTCTGCGACGCGCTGCAGGAGCGCTGCGAAGTGCCCCTGGACGTGGCGGAGGT CAGCCGCGCCTACGAGCGGCACCTCTTCGCGCCGCACGTGCCCCCCAAGCGCCGGGAGAACGGCACCT GCGAGCCCAACCCGCGTCTCAACTTCTACCCGGTCTTCGCGGTACCCGAGGTGCTGGCCACCTACCAC ATCTTTTTCCAAAACTGCAAGATCCCCCTCTCCTGCCGCGCCAACCGCACCCGCGCCGACAAAACCCTG ACCCTGCGGCAGGGCGCCCACATACCTGATATCGCCTCTCTGGAGGAAGTGCCCAAGATCTTCGAGGG TCTCGGTCGCGACGAGAAACGGGCGGCGAACGCTCTGCACGGAGACAGCGAAAACGAGAGTCACTCG GGGGTGCTGGTGGAGCTCGAGGGCGACAACGCGCGCCTGGCCGTACTCAAGCGCAGCATAGAGGTCA CCCACTTTGCCTACCCGGCGCTCAACCTGCCCCCCAAGGTCATGAGTGTGGTCATGGGCGAGCTCATC ATGCGCCGCGCCCAGCCCCTGGCCGCGGATGCAAACTTGCAAGAGTCCTCCGAGGAAGGCCTGCCCGC GGTCAGCGACGAGCAGCTGGCGCGCTGGCTGGAGACCCGCGACCCCGCGCAGCTGGAGGAGCGGCGC AAGCTCATGATGGCCGCGGTGCTGGTCACCGTGGAGCTCGAGTGTCTGCAGCGCTTCTTCGCGGACCC CGAGATGCAGCGCAAGCTCGAGGAGACCCTGCACTACACCTTCCGCCAGGGCTACGTGCGCCAGGCCT GCAAGATCTCCAACGTGGAGCTCTGCAACCTGGTCTCCTACCTGGGCATCCTGCACGAGAACCGCCTC GGGCAGAACGTCCTGCACTCCACCCTCAAAGGGGAGGCGCGCCGCGACTACATCCGCGACTGCGCCTA CCTCTTCCTCTGCTACACCTGGCAGACGGCCATGGGGGTCTGGCAGCAGTGCCTGGAGGAGCGCAACC TCAAGGAGCTGGAAAAGCTCCTCAAGCGCACCCTCAGGGACCTCTGGACGGGCTTCAACGAGCGCTCG GTGGCCGCCGCGCTGGCGGACATCATCTTTCCCGAGCGCCTGCTCAAGACCCTGCAGCAGGGCCTGCC CGACTTCACCAGCCAGAGCATGCTGCAGAACTTCAGGACTTTCATCCTGGAGCGCTCGGGCATCCTGC CGGCCACTTGCTGCGCGCTGCCCAGCGACTTCGTGCCCATCAAGTACAGGGAGTGCCCGCCGCCGCTC TGGGGCCACTGCTACCTCTTCCAGCTGGCCAACTACCTCGCCTACCACTCGGACCTCATGGAAGACGT GAGCGGCGAGGGCCTGCTCGAGTGCCACTGCCGCTGCAACCTCTGCACGCCCCACCGCTCTCTAGTCT GCAACCCGCAGCTGCTCAGCGAGAGTCAGATTATCGGTACCTTCGAGCTGCAGGGTCCCTCGCCTGAC GAGAAGTCCGCGGCTCCAGGGCTGAAACTCACTCCGGGGCTGTGGACTTCCGCCTACCTACGCAAATT TGTACCTGAGGACTACCACGCCCACGAGATCAGGTTCTACGAAGACCAATCCCGCCCGCCCAAGGCGG AGCTCACCGCCTGCGTCATCACCCAGGGGCACATCCTGGGCCAATTGCAAGCCATCAACAAAGCCCGC CGAGAGTTCTTGCTGAAAAAGGGTCGGGGGGTGTACCTGGACCCCCAGTCCGGCGAGGAGCTAAACC CGCTACCCCCGCCGCCGCCCCAGCAGCGGGACCTTGCTTCCCAGGATGGCACCCAGAAAGAAGCAGC AGCCGCCGCCGCCGCCGCAGCCATACATGCTTCTGGAGGAAGAGGAGGAGGACTGGGACAGTCAGGC AGAGGAGGTTTCGGACGAGGAGCAGGAGGAGATGATGGAAGACTGGGAGGAGGACAGCAGCCTAGA CGAGGAAGCTTCAGAGGCCGAAGAGGTGGCAGACGCAACACCATCGCCCTCGGTCGCAGCCCCCTCG CCGGGGCCCCTGAAATCCTCCGAACCCAGCACCAGCGCTATAACCTCCGCTCCTCCGGCGCCGGCGCC ACCCGCCCGCAGACCCAACCGTAGATGGGACACCACAGGAACCGGGGTCGGTAAGTCCAAGTGCCCG CCGCCGCCACCGCAGCAGCAGCAGCAGCAGCGCCAGGGCTACCGCTCGTGGCGCGGGCACAAGAACG CCATAGTCGCCTGCTTGCAAGACTGCGGGGGCAACATCTCTTTCGCCCGCCGCTTCCTGCTATTCCACC ACGGGGTCGCCTTTCCCCGCAATGTCCTGCATTACTACCGTCATCTCTACAGCCCCTACTGCAGCGGCG ACCCAGAGGCGGCAGCGGCAGCCACAGCGGCGACCACCACCTAGGAAGATATCCTCCGCGGGCAAGA CAGCGGCAGCAGCGGCCAGGAGACCCGCGGCAGCAGCGGCGGGAGCGGTGGGCGCACTGCGCCTCTC GCCCAACGAACCCCTCTCGACCCGGGAGCTCAGACACAGGATCTTCCCCACTTTGTATGCCATCTTCCA ACAGAGCAGAGGCCAGGAGCAGGAGCTGAAAATAAAAAACAGATCTCTGCGCTCCCTCACCCGCAGC TGTCTGTATCACAAAAGCGAAGATCAGCTTCGGCGCACGCTGGAGGACGCGGAGGCACTCTTCAGCAA ATACTGCGCGCTCACTCTTAAAGACTAGCTCCGCGCCCTTCTCGAATTTAGGCGGGAGAAAACTACGT CATCGCCGGCCGCCGCCCAGCCCGCCCAGCCGAGATGAGCAAAGAGATTCCCACGCCATACATGTGG AGCTACCAGCCGCAGATGGGACTCGCGGCGGGAGCGGCCCAGGACTACTCCACCCGCATGAACTACA TGAGCGCGGGACCCCACATGATCTCACAGGTCAACGGGATCCGCGCCCAGCGAAACCAAATACTGCT GGAACAGGCGGCCATCACCGCCACGCCCCGCCATAATCTCAACCCCCGAAATTGGCCCGCCGCCCTCG TGTACCAGGAAACCCCCTCCGCCACCACCGTACTACTTCCGCGTGACGCCCAGGCCGAAGTCCAGATG ACTAACTCAGGGGCGCAGCTCGCGGGCGGCTTTCGTCACGGGGCGCGGCCGCTCCGACCAGGTATAAG ACACCTGATGATCAGAGGCCGAGGTATCCAGCTCAACGACGAGTCGGTGAGCTCTTCGCTCGGTCTCC GTCCGGACGGAACTTTCCAGCTCGCCGGATCCGGCCGCTCTTCGTTCACGCCCCGCCAGGCGTACCTG ACTCTGCAGACCTCGTCCTCGGAGCCCCGCTCCGGCGGCATCGGAACCCTCCAGTTCGTGGAGGAGTT CGTGCCCTCGGTCTACTTCAACCCCTTCTCGGGACCTCCCGGACGCTACCCCGACCAGTTCATTCCGAA CTTTGACGCGGTGAAGGACTCGGCGGACGGCTACGACTGAATGTCAGGTGTCGAGGCAGAGCAGCTTC GCCTGAGACACCTCGAGCACTGCCGCCGCCACAAGTGCTTCGCCCGCGGTTCTGGTGAGTTCTGCTACT TTCAGCTACCCGAGGAGCATACCGAGGGGCCGGCGCACGGCGTCCGCCTGACCACCCAGGGCGAGGT TACCTGTTCCCTCATCCGGGAGTTTACCCTCCGTCCCCTGCTAGTGGAGCGGGAGCGGGGTCCCTGTGT CCTAACTATCGCCTGCAACTGCCCTAACCCTGGATTACATCAAGATCTTTGCTGTCATCTCTGTGCTGA GTTTAATAAACGCTGAGATCAGAATCTACTGGGGCTCCTGTCGCCATCCTGTGAACGCCACCGTCTTCA CCCACCCCGACCAGGCCCAGGCGAACCTCACCTGCGGTCTGCATCGGAGGGCCAAGAAGTACCTCACC TGGTACTTCAACGGCACCCCCTTTGTGGTTTACAACAGCTTCGACGGGGACGGAGTCTCCCTGAAAGA CCAGCTCTCCGGTCTCAGCTACTCCATCCACAAGAACACCACCCTCCAACTCTTCCCTCCCTACCTGCC GGGAACCTACGAGTGCGTCACCGGCCGCTGCACCCACCTCACCCGCCTGATCGTAAACCAGAGCTTTC CGGGAACAGATAACTCCCTCTTCCCCAGAACAGGAGGTGAGCTCAGGAAACTCCCCGGGGACCAGGG CGGAGACGTACCTTCGACCCTTGTGGGGTTAGGATTTTTTATTACCGGGTTGCTGGCTCTTTTAATCAA AGTTTCCTTGAGATTTGTTCTTTCCTTCTACGTGTATGAACACCTCAACCTCCAATAACTCTACCCTTTC TTCGGAATCAGGTGACTTCTCTGAAATCGGGCTTGGTGTGCTGCTTACTCTGTTGATTTTTTTCCTTATC ATACTCAGCCTTCTGTGCCTCAGGCTCGCCGCCTGCTGCGCACACATCTATATCTACTGCTGGTTGCTC AAGTGCAGGGGTCGCCACCCAAGATGAACAGGTACATGGTCCTATCGATCCTAGGCCTGCTGGCCCTG GCGGCCTGCAGCGCCGCCAAAAAAGAGATTACCTTTGAGGAGCCCGCTTGCAATGTAACTTTCAAGCC CGAGGGTGACCAATGCACCACCCTCGTCAAATGCGTTACCAATCATGAGAGGCTGCGCATCGACTACA AAAACAAAACTGGCCAGTTTGCGGTCTATAGTGTGTTTACGCCCGGAGACCCCTCTAACTACTCTGTCA CCGTCTTCCAGGGCGGACAGTCTAAGATATTCAATTACACTTTCCCTTTTTATGAGTTATGCGATGCGG TCATGTACATGTCAAAACAGTACAACCTGTGGCCTCCCTCTCCCCAGGCGTGTGTGGAAAATACTGGG TCTTACTGCTGTATGGCTTTCGCAATCACTACGCTCGCTCTAATCTGCACGGTGCTATACATAAAATTC AGGCAGAGGCGAATCTTTATCGATGAAAAGAAAATGCCTTGATCGCTAACACCGGCTTTCTATCTGCA GAATGAATGCAATCACCTCCCTACTAATCACCACCACCCTCCTTGCGATTGCCCATGGGTTGACACGA ATCGAAGTGCCAGTGGGGTCCAATGTCACCATGGTGGGCCCCGCCGGCAATTCCACCCTCATGTGGGA AAAATTTGTCCGCAATCAATGGGTTCATTTCTGCTCTAACCGAATCAGTATCAAGCCCAGAGCCATCTG CGATGGGCAAAATCTAACTCTGATCAATGTGCAAATGATGGATGCTGGGTACTATTACGGGCAGCGGG GAGAAATCATTAATTACTGGCGACCCCACAAGGACTACATGCTGCATGTAGTCGAGGCACTTCCCACT ACCACCCCCACTACCACCTCTCCCACCACCACCACCACTACTACTACTACTACTACTACTACTACTACT ACCACTACCGCTGCCCGCCATACCCGCAAAAGCACCATGATTAGCACAAAGCCCCCTCGTGCTCACTC CCACGCCGGCGGGCCCATCGGTGCGACCTCAGAAACCACCGAGCTTTGCTTCTGCCAATGCACTAACG

CCAGCGCTCATGAACTGTTCGACCTGGAGAATGAGGATGTCCAGCAGAGCTCCGCTTGCCTGACCCAG GAGGCTGTGGAGCCCGTTGCCCTGAAGCAGATCGGTGATTCAATAATTGACTCTTCTTCTTTTGCCACT CCCGAATACCCTCCCGATTCTACTTTCCACATCACGGGTACCAAAGACCCTAACCTCTCTTTCTACCTG ATGCTGCTGCTCTGTATCTCTGTGGTCTCTTCCGCGCTGATGTTACTGGGGATGTTCTGCTGCCTGATCT GCCGCAGAAAGAGAAAAGCTCGCTCTCAGGGCCAACCACTGATGCCCTTCCCCTACCCCCCGGATTTT GCAGATAACAAGATATGAGCTCGCTGCTGACACTAACCGCTTTACTAGCCTGCGCTCTAACCCTTGTCG CTTGCGACTCGAGATTCCACAATGTCACAGCTGTGGCAGGAGAAAATGTTACTTTCAACTCCACGGCC GATACCCAGTGGTCGTGGAGTGGCTCAGGTAGCTACTTAACTATCTGCAATAGCTCCACTTCCCCCGGC ATATCCCCAACCAAGTACCAATGCAATGCCAGCCTGTTCACCCTCATCAACGCTTCCACCCTGGACAAT GGACTCTATGTAGGCTATGTACCCTTTGGTGGGCAAGGAAAGACCCACGCTTACAACCTGGAAGTTCG CCAGCCCAGAACCACTACCCAAGCTTCTCCCACCACCACCACCACCACCACCATCACCAGCAGCAGCA GCAGCAGCAGCCACAGCAGCAGCAGCAGATTATTGACTTTGGTTTTGGCCAGCTCATCTGCCGCTACC CAGGCCATCTACAGCTCTGTGCCCGAAACCACTCAGATCCACCGCCCAGAAACGACCACCGCCACCAC CCTACACACCTCCAGCGATCAGATGCCGACCAACATCACCCCCTTGGCTCTTCAAATGGGACTTACAA GCCCCACTCCAAAACCAGTGGATGCGGCCGAGGTCTCCGCCCTCGTCAATGACTGGGCGGGGCTGGGA ATGTGGTGGTTCGCCATAGGCATGATGGCGCTCTGCCTGCTTCTGCTCTGGCTCATCTGCTGCCTCCAC CGCAGGCGAGCCAGACCCCCCATCTATAGACCCATCATTGTCCTGAACCCCGATAATGATGGGATCCA TAGATTGGATGGCCTGAAAAACCTACTTTTTTCTTTTACAGTATGATAAATTGAGACATGCCTCGCATT TTCTTGTACATGTTCCTTCTCCCACCTTTTCTGGGGTGTTCTACGCTGGCCGCTGTGTCTCACCTGGAGG TAGACTGCCTCTCACCCTTCACTGTCTACCTGCTTTACGGATTGGTCACCCTCACTCTCATCTGCAGCCT AATCACAGTAATCATCGCCTTCATCCAGTGCATTGATTACATCTGTGTGCGCCTCGCATACTTCAGACA CCACCCGCAGTACCGAGACAGGAACATTGCCCAACTTCTAAGACTGCTCTAATCATGCATAAGACTGT GATCTGCCTTCTGATCCTCTGCATCCTGCCCACCCTCACCTCCTGCCAGTACACCACAAAATCTCCGCG CAAAAGACATGCCTCCTGCCGCTTCACCCAACTGTGGAATATACCCAAATGCTACAACGAAAAGAGCG AGCTCTCCGAAGCTTGGCTGTATGGGGTCATCTGTGTCTTAGTTTTCTGCAGCACTGTCTTTGCCCTCAT AATCTACCCCTACTTTGATTTGGGATGGAACGCGATCGATGCCATGAATTACCCCACCTTTCCCGCACC CGAGATAATTCCACTGCGACAAGTTGTACCCGTTGTCGTTAATCAACGCCCCCCATCCCCTACGCCCAC TGAAATCAGCTACTTTAACCTAACAGGCGGAGATGACTGACGCCCTAGATCTAGAAATGGACGGCATC AGTACCGAGCAGCGTCTCCTAGAGAGGCGCAGGCAGGCGGCTGAGCAAGAGCGCCTCAATCAGGAGC TCCGAGATCTCGTTAACCTGCACCAGTGCAAAAGAGGCATCTTTTGTCTGGTAAAGCAGGCCAAAGTC ACCTACGAGAAGACCGGCAACAGCCACCGCCTCAGTTACAAATTGCCCACCCAGCGCCAGAAGCTGG TGCTCATGGTGGGTGAGAATCCCATCACCGTCACCCAGCACTCGGTAGAGACCGAGGGGTGTCTGCAC TCCCCCTGTCGGGGTCCAGAAGACCTCTGCACCCTGGTAAAGACCCTGTGCGGTCTCAGAGATTTAGT CCCCTTTAACTAATCAAACACTGGAATCAATAAAAAGAATCACTTACTTAAAATCAGACAGCAGGTCT CTGTCCAGTTTATTCAGCAGCACCTCCTTCCCCTCCTCCCAACTCTGGTACTCCAAACGCCTTCTGGCG GCAAACTTCCTCCACACCCTGAAGGGAATGTCAGATTCTTGCTCCTGTCCCTCCGCACCCACTATCTTC ATGTTGTTGCAGATGAAGCGCACCAAAACGTCTGACGAGAGCTTCAACCCCGTGTACCCCTATGACAC GGAAAGCGGCCCTCCCTCCGTCCCTTTCCTCACCCCTCCCTTCGTGTCTCCCGATGGATTCCAAGAAAG TCCCCCCGGGGTCCTGTCTCTGAACCTGGCCGAGCCCCTGGTCACTTCCCACGGCATGCTCGCCCTGAA AATGGGAAGTGGCCTCTCCCTGGACGACGCTGGCAACCTCACCTCTCAAGATATCACCACCGCTAGCC CTCCCCTCAAAAAAACCAAGACCAACCTCAGCCTAGAAACCTCATCCCCCCTAACTGTGAGCACCTCA GGCGCCCTCACCGTAGCAGCCGCCGCTCCCCTGGCGGTGGCCGGCACCTCCCTCACCATGCAATCAGA GGCCCCCCTGACAGTACAGGATGCAAAACTCACCCTGGCCACCAAAGGCCCCCTGACCGTGTCTGAAG GCAAACTGGCCTTGCAAACATCGGCCCCGCTGACGGCCGCTGACAGCAGCACCCTCACAGTCAGTGCC ACACCACCCCTTAGCACAAGCAATGGCAGCTTGGGTATTGACATGCAAGCCCCCATTTACACCACCAA TGGAAAACTAGGACTTAACTTTGGCGCTCCCCTGCATGTGGTAGACAGCCTAAATGCACTGACTGTAG TTACTGGCCAAGGTCTTACGATAAACGGAACAGCCCTACAAACTAGAGTCTCAGGTGCCCTCAACTAT GACACATCAGGAAACCTAGAATTGAGAGCTGCAGGGGGTATGCGAGTTGATGCAAATGGTCAACTTA TCCTTGATGTAGCTTACCCATTTGATGCACAAAACAATCTCAGCCTTAGGCTTGGACAGGGACCCCTGT TTGTTAACTCTGCCCACAACTTGGATGTTAACTACAACAGAGGCCTCTACCTGTTCACATCTGGAAATA CCAAAAAGCTAGAAGTTAATATCAAAACAGCCAAGGGTCTCATTTATGATGACACTGCTATAGCAATC AATGCGGGTGATGGGCTACAGTTTGACTCAGGCTCAGATACAAATCCATTAAAAACTAAACTTGGATT AGGACTGGATTATGACTCCAGCAGAGCCATAATTGCTAAACTGGGAACTGGCCTAAGCTTTGACAACA CAGGTGCCATCACAGTAGGCAACAAAAATGATGACAAGCTTACCTTGTGGACCACACCAGACCCATCC CCTAACTGTAGAATCTATTCAGAGAAAGATGCTAAATTCACACTTGTTTTGACTAAATGCGGCAGTCA GGTGTTGGCCAGCGTTTCTGTTTTATCTGTAAAAGGTAGCCTTGCGCCCATCAGTGGCACAGTAACTAG TGCTCAGATTGTCCTCAGATTTGATGAAAATGGAGTTCTACTAAGCAATTCTTCCCTTGACCCTCAATA CTGGAACTACAGAAAAGGTGACCTTACAGAGGGCACTGCATATACCAACGCAGTGGGATTTATGCCCA ACCTCACAGCATACCCAAAAACACAGAGCCAAACTGCTAAAAGCAACATTGTAAGTCAGGTTTACTTG AATGGGGACAAATCCAAACCCATGACCCTCACCATTACCCTCAATGGAACTAATGAAACAGGAGATG CCACAGTAAGCACTTACTCCATGTCATTCTCATGGAACTGGAATGGAAGTAATTACATTAATGAAACG TTCCAAACCAACTCCTTCACCTTCTCCTACATCGCCCAAGAATAAAAAGCATGACGCTGTTGATTTGAT TCAATGTGTTTCTGTTTTATTTTCAAGCACAACAAAATCATTCAAGTCATTCTTCCATCTTAGCTTAATA GACACAGTAGCTTAATAGACCCAGTAGTGCAAAGCCCCATTCTAGCTTATAACTAGTGGAGAAGTACT CGCCTACATGGGGGTAGAGTCATAATCGTGCATCAGGATAGGGCGGTGGTGCTGCAGCAGCGCGCGA ATAAACTGCTGCCGCCGCCGCTCCGTCCTGCAGGAATACAACATGGCAGTGGTCTCCTCAGCGATGAT TCGCACCGCCCGCAGCATAAGGCGCCTTGTCCTCCGGGCACAGCAGCGCACCCTGATCTCACTTAAAT CAGCACAGTAACTGCAGCACAGCACCACAATATTGTTCAAAATCCCACAGTGCAAGGCGCTGTATCCA AAGCTCATGGCGGGGACCACAGAACCCACGTGGCCATCATACCACAAGCGCAGGTAGATTAAGTGGC GACCCCTCATAAACACGCTGGACATAAACATTACCTCTTTTGGCATGTTGTAATTCACCACCTCCCGGT ACCATATAAACCTCTGATTAAACATGGCGCCATCCACCACCATCCTAAACCAGCTGGCCAAAACCTGC CCGCCGGCTATACACTGCAGGGAACCGGGACTGGAACAATGACAGTGGAGAGCCCAGGACTCGTAAC CATGGATCATCATGCTCGTCATGATATCAATGTTGGCACAACACAGGCACACGTGCATACACTTCCTC AGGATTACAAGCTCCTCCCGCGTTAGAACCATATCCCAGGGAACAACCCATTCCTGAATCAGCGTAAA TCCCACACTGCAGGGAAGACCTCGCACGTAACTCACGTTGTGCATTGTCAAAGTGTTACATTCGGGCA GCAGCGGATGATCCTCCAGTATGGTAGCGCGGGTTTCTGTCTCAAAAGGAGGTAGACGATCCCTACTG TACGGAGTGCGCCGAGACAACCGAGATCGTGTTGGTCGTAGTGTCATGCCAAATGGAACGCCGGACGT AGTCATATTTCCTGAAGTCTTAGATCTCTCAACGCAGCACCAGCACCAACACTTCGCAGTGTAAAAGG CCAAGTGCCGAGAGAGTATATATAGGAATAAAAAGTGACGTAAACGGGCAAAGTCCAAAAAACGCCC AGAAAAACCGCACGCGAACCTACGCCCCGAAACGAAAGCCAAAAAACACTAGACACTCCCTTCCGGC GTCAACTTCCGCTTTCCCACGCTACGTCACTTGCCCCAGTCAAACAAACTACATATCCCGAACTTCCAA GTCGCCACGCCCAAAACACCGCCTACACCTCCCCGCCCGCCGGCCCGCCCCCAAACCCGCCTCCCGCC CCGCGCCCCGCCCCGCGCCGCCCATCTCATTATCATATTGGCTTCAATCCAAAATAAGGTATATTATTG ATGATG RSV F0.DELTA.TM-N-M2-1 amino acid sequence SEQ ID NO: 5 MELLILKANAITTILTAVTFCFASGQNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDA KVKLIKQELDKYKNAVTELQLLMQSTPATNNRARRELPRFMNYTLNNAKKTNVTLSKKRKRRFLGFLLGV GSAIASGVAVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQLLPIVNKQSCSISNI ETVIEFQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLNDMPITNDQKKLMSNNVQIVRQQSYSIMSI IKEEVLAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQS NRVECDTMNSLTLPSEVNLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTF SNGCDYVSNKGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIR KSDELLHNVNAGKSTTNRKRRAPVKQTLNFDLLKLAGDVESNPGPMALSKVKLNDTLNKDQLLSSSKYTI QRSTGDSIDTPNYDVQKHINKLCGMLLITEDANHKFTGLIGMLYAMSRLGREDTIKILRDAGYHVKANGVD VTTHRQDINGKEMKFEVLTLASLTTEIQINIEIESRKSYKKMLKEMGEVAPEYRHDSPDCGMIILCIAALVIT KLAAGDRSGLTAVIRRANNVLKNEMKRYKGLLPKDIANS FYEVFEKYPHFIDVFVHFGIAQSSTRGGSRVEGIFAGLEMNAYGAGQVMLRWGVLAKSVKNIMLGHASVQ AEMEQVVEVYLYAQKLGGEAGFYHILNNPKASLLSLTQFPHFSSVVLGNAAGLGIMGEYRGTPRNQDLYD AAKAYAEQLKENGVINYSVLDLTAEELEAIKHQLNPKDNDVELGGGGSGGGGMSRRNPCKFEIRGHCLNG KRCHFSHNYFEWPPHALLVRQNFMLNRILKSMDKSIDTLSEISGAAELDRTEEYALGVVGVLESYIGSINNIT KQSACVAMSKLLTELNSDDIKKLRDNEELNSPKIRVYNTVISYIESNRKNNKQTIHLLKRLPADVLKKTIKN TLDIHKSITINNPKESTVSDTNDHAKNNDTT Polynucleotide sequence encoding the enhanced hCMV promoter SEQ ID NO: 6 CCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTG ACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCC GCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATG ACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCG CCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCT ATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAG TCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGGCGAAGCGCTCCCTAT CAGTGATAGAGATCTCCCTATCAGTGATAGAGATCGTCGACGAGCTCGCGGCGGGCGGGAGTCGCTGCGCGCTG CCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTAAAAC AGGTAAGTCCGGCCTCCGCGCCGGGTTTTGGCGCCTCCCGCGGGCGCCCCCCTCCTCACGGCGAGCGCTGCCAC GTCAGACGAAGGGCGCAGCGAGCGTCCTGATCCTTCCGCCCGGACGCTCAGGACAGCGGCCCGCTGCTCATAAG ACTCGGCCTTAGAACCCCAGTATCAGCAGAAGGACATTTTAGGACGGGACTTGGGTGACTCTAGGGCACTGGTT TTCTTTCCAGAGAGCGGAACAGGCGAGGAAAAGTAGTCCCTTCTCGGCGATTCTGCGGAGGGATCTCCGTGGGG

CGGTGAACGCCGATGATGCCTCTACTAACCATGTTCATGTTTTCTTTTTTTTTCTACAGGTCCTGGGTGACGAA CAG Polynucleotide sequence encoding the hCMV NM2 bghpolyA cassette SEQ ID NO: 7 CCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTG ACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCC GCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATG ACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCG CCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCT ATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAG TCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCCCTATC AGTGATAGAGATCTCCCTATCAGTGATAGAGATCGTCGACGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAG ACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGCCGGGAACGGTGCA TTGGAACGCGGATTCCCCGTGCCAAGAGTGAGATCTTCCGTTTATCTAGGTACCAGATATCGCCACCATGGCCC TGAGCAAAGTGAAACTGAACGATACACTGAACAAGGACCAGCTGCTGTCCAGCAGCAAGTACACCATCCAGCGG AGCACCGGCGACAGCATCGATACCCCCAACTACGACGTGCAGAAGCACATCAACAAGCTGTGCGGCATGCTGCT GATCACAGAGGACGCCAACCACAAGTTCACCGGCCTGATCGGCATGCTGTACGCCATGAGCCGGCTGGGCCGGG AGGACACCATCAAGATCCTGCGGGACGCCGGCTACCACGTGAAGGCCAATGGCGTGGACGTGACCACACACCGG CAGGACATCAACGGCAAAGAAATGAAGTTCGAGGTGCTGACCCTGGCCAGCCTGACCACCGAGATCCAGATCAA TATCGAGATCGAGAGCCGGAAGTCCTACAAGAAAATGCTGAAAGAAATGGGCGAGGTGGCCCCCGAGTACAGAC ACGACAGCCCCGACTGCGGCATGATCATCCTGTGTATCGCCGCCCTGGTGATCACAAAGCTGGCCGCTGGCGAC AGATCTGGCCTGACAGCCGTGATCAGACGGGCCAACAATGTGCTGAAGAACGAGATGAAGCGGTACAAGGGCCT GCTGCCCAAGGACATTGCCAACAGCTTCTACGAGGTGTTCGAGAAGTACCCCCACTTCATCGACGTGTTCGTGC ACTTCGGCATTGCCCAGAGCAGCACCAGAGGCGGCTCCAGAGTGGAGGGCATCTTCGCCGGCCTGTTCATGAAC GCCTACGGCGCTGGCCAGGTGATGCTGAGATGGGGCGTGCTGGCCAAGAGCGTGAAGAACATCATGCTGGGCCA CGCCAGCGTGCAGGCCGAGATGGAACAGGTGGTGGAGGTGTACGAGTACGCCCAGAAGCTGGGCGGAGAGGCCG GCTTCTACCACATCCTGAACAACCCTAAGGCCTCCCTGCTGTCCCTGACCCAGTTCCCCCACTTCTCCAGCGTG GTGCTGGGAAATGCCGCCGGACTGGGCATCATGGGCGAGTACCGGGGCACCCCCAGAAACCAGGACCTGTACGA CGCCGCCAAGGCCTACGCCGAGCAGCTGAAAGAAAACGGCGTGATCAACTACAGCGTGCTGGACCTGACCGCTG AGGAACTGGAAGCCATCAAGCACCAGCTGAACCCCAAGGACAACGACGTGGAGCTGGGAGGCGGAGGATCTGGC GGCGGAGGCATGAGCAGACGGAACCCCTGCAAGTTCGAGATCCGGGGCCACTGCCTGAACGGCAAGCGGTGCCA CTTCAGCCACAACTACTTCGAGTGGCCCCCTCATGCTCTGCTGGTGCGGCAGAACTTCATGCTGAACCGGATCC TGAAGTCCATGGACAAGAGCATCGACACCCTGAGCGAGATCAGCGGAGCCGCCGAGCTGGACAGAACCGAGGAA TATGCCCTGGGCGTGGTGGGAGTGCTGGAAAGCTACATCGGCTCCATCAACAACATCACAAAGCAGAGCGCCTG CGTGGCCATGAGCAAGCTGCTGACAGAGCTGAACAGCGACGACATCAAGAAGCTGAGGGACAACGAGGAACTGA ACAGCCCCAAGATCCGGGTGTACAACACCGTGATCAGCTACATTGAGAGCAACCGCAAGAACAACAAGCAGACC ATCCATCTGCTGAAGCGGCTGCCCGCCGACGTGCTGAAAAAGACCATCAAGAACACCCTGGACATCCACAAGTC CATCACCATCAACAATCCCAAAGAAAGCACCGTGTCTGACACCAACGATCACGCCAAGAACAACGACACCACCT GATGAGCGGCCGCGATCTGCTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTT GACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGT GTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCT GGGGATGCGGTGGGCTCTATGG CMV Promoter sequence: bold Transgene sequence NM2: Italic bghpolyA PolyA signal: italic + underline NM2 protein sequence SEQ ID NO: 8 MALSKVKLNDTLNKDQLLSSSKYTIQRSTGDSIDTPNYDVQKHINKLCGMLLITEDANHKFTGLIGMLYAMSRL GREDTIKILRDAGYHVKANGVDVTTHRQDINGKEMKFEVLTLASLTTEIQINIEIESRKSYKKMLKEMGEVAPE YRHDSPDCGMIILCIAALVITKLAAGDRSGLTAVIRRANNVLKNEMKRYKGLLPKDIANSFYEVFEKYPHFIDV FVHFGIAQSSTRGGSRVEGIFAGLFMNAYGAGQVMLRWGVLAKSVKNIMLGHASVQAEMEQVVEVYEYAQKLGG EAGFYHILNNPKASLLSLTQFPHFSSVVLGNAAGLGIMGEYRGTPRNQDLYDAAKAYAEQLKENGVINYSVLDL TAEELEAIKHQLNPKDNDVELGGGGSGGGGMSRRNPCKFEIRGHCLNGKRCHFSHNYFEWPPHALLVRQNFMLN RILKSMDKSIDTLSEISGAAELDRTEEYALGVVGVLESYIGSINNITKQSACVAMSKLLTELNSDDIKKLRDNE ELNSPKIRVYNTVISYIESNRKNNKQTIHLLKRLPADVLKKTIKNTLDIHKSITINNPKESTVSDTNDHAKNND TT Polynucleotide sequence encoding the hCMV F0 WPRE bghpolyA cassette SEQ ID NO: 9 CCATTGCATACGTTGTATCCATATCATAATATGTACATTTATATTGGCTCATGTCCAACATTACCGCCATGTTG ACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCC GCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATG ACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCG CCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCT ATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAG TCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACA ACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGGCGAAGCGCTCCCTAT CAGTGATAGAGATCTCCCTATCAGTGATAGAGATCGTCGACGAGCTCGCGGCGGGCGGGAGTCGCTGCGCGCTG CCTTCGCCCCGTGCCCCGCTCCGCCGCCGCCTCGCGCCGCCCGCCCCGGCTCTGACTGACCGCGTTACTAAAAC AGGTAAGTCCGGCCTCCGCGCCGGGTTTTGGCGCCTCCCGCGGGCGCCCCCCTCCTCACGGCGAGCGCTGCCAC GTCAGACGAAGGGCGCAGCGAGCGTCCTGATCCTTCCGCCCGGACGCTCAGGACAGCGGCCCGCTGCTCATAAG ACTCGGCCTTAGAACCCCAGTATCAGCAGAAGGACATTTTAGGACGGGACTTGGGTGACTCTAGGGCACTGGTT TTCTTTCCAGAGAGCGGAACAGGCGAGGAAAAGTAGTCCCTTCTCGGCGATTCTGCGGAGGGATCTCCGTGGGG CGGTGAACGCCGATGATGCCTCTACTAACCATGTTCATGTTTTCTTTTTTTTTCTACAGGTCCTGGGTGACGAA CAGGATATCGCCACCATGGAACTGCTGATCCTGAAGGCCAACGCCATCACCACCATCCTGACCGCCGTGACCTT CTGCTTCGCCAGCGGCCAGAACATCACCGAGGAATTCTACCAGAGCACCTGTAGCGCCGTGAGCAAGGGCTACC TGAGCGCCCTGAGAACCGGCTGGTACACCAGCGTGATCACCATCGAGCTGAGCAACATCAAAGAAAACAAGTGC AACGGCACCGACGCCAAAGTGAAGCTGATCAAGCAGGAACTGGACAAGTACAAGAACGCCGTGACCGAGCTGCA GCTGCTGATGCAGAGCACCCCCGCCACCAACAACCGGGCCAGACGGGAGCTGCCCCGGTTCATGAACTACACCC TGAACAACGCCAAAAAGACCAACGTGACCCTGAGCAAGAAGCGGAAGCGGCGGTTCCTGGGCTTTCTGCTGGGC GTGGGCAGCGCCATTGCCAGCGGCGTGGCCGTGTCTAAGGTGCTGCACCTGGAAGGCGAAGTGAACAAGATCAA GAGCGCCCTGCTGAGCACCAACAAGGCCGTGGTGTCCCTGAGCAACGGCGTGAGCGTGCTGACCAGCAAGGTGC TGGATCTGAAGAACTACATCGACAAGCAGCTGCTGCCCATCGTGAACAAGCAGAGCTGCAGCATCAGCAACATC GAGACAGTGATCGAGTTCCAGCAGAAGAACAACCGGCTGCTGGAAATCACCCGGGAGTTCAGCGTGAACGCCGG CGTGACCACCCCTGTGTCCACCTACATGCTGACCAACAGCGAGCTGCTGAGCCTGATCAACGACATGCCCATCA CCAACGACCAGAAAAAGCTGATGAGCAACAACGTGCAGATCGTGCGGCAGCAGAGCTACTCCATCATGTCCATC ATCAAAGAAGAGGTGCTGGCCTACGTGGTGCAGCTGCCCCTGTACGGCGTGATCGACACCCCCTGCTGGAAGCT GCACACCAGCCCCCTGTGCACCACCAACACCAAAGAGGGCAGCAACATCTGCCTGACCCGGACCGACAGAGGCT GGTACTGCGACAACGCCGGCAGCGTGTCATTCTTTCCACAGGCCGAGACATGCAAGGTGCAGAGCAACCGGGTG

TTCTGCGACACCATGAACAGCCTGACCCTGCCCTCCGAAGTGAACCTGTGCAACGTGGACATCTTCAACCCCAA GTACGACTGCAAGATCATGACCTCCAAGACCGACGTGTCCAGCTCCGTGATCACCTCCCTGGGCGCCATCGTGT CCTGCTACGGCAAGACCAAGTGCACCGCCAGCAACAAGAACCGGGGCATCATCAAGACCTTCAGCAACGGCTGC GACTACGTGTCCAACAAGGGGGTGGACACCGTGTCCGTGGGCAACACCCTGTACTACGTGAACAAACAGGAAGG CAAGAGCCTGTACGTGAAGGGCGAGCCCATCATCAACTTCTACGACCCCCTGGTGTTCCCCAGCGACGAGTTCG ACGCCAGCATCAGCCAGGTGAACGAGAAGATCAACCAGAGCCTGGCCTTCATCCGGAAGTCCGACGAGCTGCTG CACAATGTGAATGCCGGCAAGTCCACCACCAACTGATGAGCGGCCATCTAATCAACCTCTGGATTACAAAATTT GTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTG TATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGA GGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGG GCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATC GCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAA ATCATCGTCCTTTCCTTGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCC CTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGC CTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGCGGCCGCGATCTGCTGTGCCTTCTA GTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTT TCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCA GGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG Enhanced CMV Promoter sequence: bold Transgene sequence F0: Italic WPRE sequence: underlined bold bghpolyA PolyA signal: italic + underline F0 protein sequence SEQ ID NO: 10 MELLILKANAITTILTAVTFCFASGQNITEEFYQSTCSAVSKGYLSALRTGWYTSVITIELSNIKENKCNGTDA KVKLIKQELDKYKNAVTELQLLMQSTPATNNRARRELPRFMNYTLNNAKKTNVTLSKKRKRRFLGFLLGVGSAI ASGVAVSKVLHLEGEVNKIKSALLSTNKAVVSLSNGVSVLTSKVLDLKNYIDKQLLPIVNKQSCSISNIETVIE FQQKNNRLLEITREFSVNAGVTTPVSTYMLTNSELLSLINDMPITNDQKKLMSNNVQIVRQQSYSIMSIIKEEV LAYVVQLPLYGVIDTPCWKLHTSPLCTTNTKEGSNICLTRTDRGWYCDNAGSVSFFPQAETCKVQSNRVFCDTM NSLTLPSEVNLCNVDIFNPKYDCKIMTSKTDVSSSVITSLGAIVSCYGKTKCTASNKNRGIIKTFSNGCDYVSN KGVDTVSVGNTLYYVNKQEGKSLYVKGEPIINFYDPLVFPSDEFDASISQVNEKINQSLAFIRKSDELLHNVNA GKSTTN Amino acid sequence of a flexible linker SEQ ID NO: 11 Gly-Gly-Gly-Ser-Gly-Gly-Gly Amino acid sequence of a flexible linker SEQ ID NO: 12 Gly-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Gly

Sequence CWU 1

1

12137830DNAChimpanzee adenovirus 1catcatcaat aatatacctt attttggatt gaagccaata tgataatgag atgggcggcg 60cggggcggga ggcgggtccg ggggcgggcc ggcgggcggg gcggtgtggc ggaagtggac 120tttgtaagtg tggcggatgt gacttgctag tgccgggcgc ggtaaaagtg acgttttccg 180tgcgcgacaa cgcccacggg aagtgacatt tttcccgcgg tttttaccgg atgttgtagt 240gaatttgggc gtaaccaagt aagatttggc cattttcgcg ggaaaactga aacggggaag 300tgaaatctga ttaatttcgc gttagtcata ccgcgtaata tttgtcgagg gccgagggac 360tttggccgat tacgtggagg actcgcccag gtgttttttg aggtgaattt ccgcgttccg 420ggtcaaagtc tccgttttat tattatagtc agctgacgcg gagtgtattt ataccctctg 480atctcgtcaa gtggccactc ttgagtgcca gcgagtagag ttttctcctc tgccgctctc 540cgctccgctc cgctcggctc tgacaccggg gaaaaaatga gacatttcac ctacgatggc 600ggtgtgctca ccggccagct ggctgctgaa gtcctggaca ccctgatcga ggaggtattg 660gccgataatt atcctccctc gactcctttt gagccaccta cacttcacga actctacgat 720ctggatgtgg tggggcccag cgatccgaac gagcaggcgg tttccagttt ttttccagag 780tccatgttgt tggccagcca ggagggggtc gaacttgaga cccctcctcc gatcgtggat 840tcccccgatc cgccgcagct gactaggcag cccgagcgct gtgcgggacc tgagactatg 900ccccagctgc tacctgaggt gatcgatctc acctgtaatg agtctggttt tccacccagc 960gaggatgagg acgaagaggg tgagcagttt gtgttagatt ctgtggaaca acccgggcga 1020ggatgcaggt cttgtcaata tcaccggaaa aacacaggag actcccagat tatgtgttct 1080ctgtgttata tgaagatgac ctgtatgttt atttacagta agtttatcat ctgtgggcag 1140gtgggctata gtgtgggtgg tggtctttgg ggggtttttt aatatatgtc aggggttatg 1200ctgaagactt ttttattgtg atttttaaag gtccagtgtc tgagcccgag caagaacctg 1260aaccggagcc tgagccttct cgccccagga gaaagcctgt aatcttaact agacccagcg 1320caccggtagc gagaggcctc agcagcgcgg agaccaccga ctccggtgct tcctcatcac 1380ccccggagat tcaccccctg gtgcccctgt gtcccgttaa gcccgttgcc gtgagagtca 1440gtgggcggcg gtctgctgtg gagtgcattg aggacttgct ttttgattca caggaacctt 1500tggacttgag cttgaaacgc cccaggcatt aaacctggtc acctggactg aatgagttga 1560cgcctatgtt tgcttttgaa tgacttaatg tgtatagata ataaagagtg agataatgtt 1620ttaattgcat ggtgtgttta acttgggcgg agtctgctgg gtatataagc ttccctgggc 1680taaacttggt tacacttgac ctcatggagg cctgggagtg tttggagaac tttgccggag 1740ttcgtgcctt gctggacgag agctctaaca atacctcttg gtggtggagg tatttgtggg 1800gctctcccca gggcaagtta gtttgtagaa tcaaggagga ttacaagtgg gaatttgaag 1860agcttttgaa atcctgtggt gagctattgg attctttgaa tctaggccac caggctctct 1920tccaggagaa ggtcatcagg actttggatt tttccacacc ggggcgcatt gcagccgcgg 1980ttgcttttct agcttttttg aaggatagat ggagcgaaga gacccacttg agttcgggct 2040acgtcctgga ttttctggcc atgcaactgt ggagagcatg gatcagacac aagaacaggc 2100tgcaactgtt gtcttccgtc cgcccgttgc tgattccggc ggaggagcaa caggccgggt 2160cagaggaccg ggcccgtcgg gatccggagg agagggcacc gaggccgggc gagaggagcg 2220cgctgaacct gggaaccggg ctgagcggcc atccacatcg ggagtgaatg tcgggcaggt 2280ggtggatctt tttccagaac tgcggcggat tttgactatt agggaggatg ggcaatttgt 2340taagggtctt aagagggaga ggggggcttc tgagcataac gaggaggcca gtaatttagc 2400ttttagcttg atgaccagac accgtccaga gtgcatcact tttcagcaga ttaaggacaa 2460ttgtgccaat gagttggatc tgttgggtca gaagtatagc atagagcagc tgaccactta 2520ctggctgcag ccgggtgatg atctggagga agctattagg gtgtatgcta aggtggccct 2580gcggcccgat tgcaagtaca agctcaaggg gctggtgaat atcaggaatt gttgctacat 2640ttctggcaac ggggcggagg tggagataga gaccgaagac agggtggctt tcagatgcag 2700catgatgaat atgtggccgg gggtgctggg catggacggg gtggtgatta tgaatgtgag 2760gttcacgggg cccaacttta acggcacggt gtttttgggg aacaccaacc tggtcctgca 2820cggggtgagc ttctatgggt ttaacaacac ctgtgtggag gcctggaccg atgtgaaggt 2880ccgcggttgc gccttttatg gatgttggaa ggccatagtg agccgcccta agagcaggag 2940ttccattaag aaatgcttgt ttgagaggtg caccttgggg atcctggccg agggcaactg 3000cagggtgcgc cacaatgtgg cctccgagtg cggttgcttc atgctagtca agagcgtggc 3060ggtaatcaag cataatatgg tgtgcggcaa cagcgaggac aaggcctcac agatgctgac 3120ctgcacggat ggcaactgcc acttgctgaa gaccatccat gtaaccagcc acagccggaa 3180ggcctggccc gtgttcgagc acaacttgct gacccgctgc tccttgcatc tgggcaacag 3240gcggggggtg ttcctgccct atcaatgcaa ctttagtcac accaagatct tgctagagcc 3300cgagagcatg tccaaggtga acttgaacgg ggtgtttgac atgaccatga agatctggaa 3360ggtgctgagg tacgacgaga ccaggtcccg gtgcagaccc tgcgagtgcg ggggcaagca 3420tatgaggaac cagcccgtga tgctggatgt gaccgaggag ctgaggacag accacttggt 3480tctggcctgc accagggccg agtttggttc tagcgatgaa gacacagatt gaggtgggtg 3540agtgggcgtg gcctggggtg gtcatgaaaa tatataagtt gggggtctta gggtctcttt 3600atttgtgttg cagagaccgc cggagccatg agcgggagca gcagcagcag cagtagcagc 3660agcgccttgg atggcagcat cgtgagccct tatttgacga cgcggatgcc ccactgggcc 3720ggggtgcgtc agaatgtgat gggctccagc atcgacggcc gacccgtcct gcccgcaaat 3780tccgccacgc tgacctatgc gaccgtcgcg gggacgccgt tggacgccac cgccgccgcc 3840gccgccaccg cagccgcctc ggccgtgcgc agcctggcca cggactttgc attcctggga 3900ccactggcga caggggctac ttctcgggcc gctgctgccg ccgttcgcga tgacaagctg 3960accgccctgc tggcgcagtt ggatgcgctt actcgggaac tgggtgacct ttctcagcag 4020gtcatggccc tgcgccagca ggtctcctcc ctgcaagctg gcgggaatgc ttctcccaca 4080aatgccgttt aagataaata aaaccagact ctgtttggat taaagaaaag tagcaagtgc 4140attgctctct ttatttcata attttccgcg cgcgataggc cctagaccag cgttctcggt 4200cgttgagggt gcggtgtatc ttctccagga cgtggtagag gtggctctgg acgttgagat 4260acatgggcat gagcccgtcc cgggggtgga ggtagcacca ctgcagagct tcatgctccg 4320gggtggtgtt gtagatgatc cagtcgtagc aggagcgctg ggcatggtgc ctaaaaatgt 4380ccttcagcag caggccgatg gccaggggga ggcccttggt gtaagtgttt acaaaacggt 4440taagttggga agggtgcatt cggggagaga tgatgtgcat cttggactgt atttttagat 4500tggcgatgtt tccgcccaga tcccttctgg gattcatgtt gtgcaggacc accagtacag 4560tgtatccggt gcacttgggg aatttgtcat gcagcttaga gggaaaagcg tggaagaact 4620tggagacgcc tttgtggcct cccagatttt ccatgcattc gtccatgatg atggcaatgg 4680gcccgcggga ggcagcttgg gcaaagatat ttctggggtc gctgacgtcg tagttgtgtt 4740ccagggtgag gtcgtcatag gccattttta caaagcgcgg gcggagggtg cccgactggg 4800ggatgatggt cccctctggc cctggggcgt agttgccctc gcagatctgc atttcccagg 4860ccttaatctc ggagggggga atcatatcca cctgcggggc gatgaagaaa acggtttccg 4920gagccgggga gattaactgg gatgagagca ggtttctaag cagctgtgat tttccacaac 4980cggtgggccc ataaataaca cctataaccg gttgcagctg gtagtttaga gagctgcagc 5040tgccgtcgtc ccggaggagg ggggccacct cgttgagcat gtccctgacg cgcatgttct 5100ccccgaccag atccgccaga aggcgctcgc cgcccaggga cagcagctct tgcaaggaag 5160caaagttttt cagcggcttg aggccgtccg ccgtgggcat gtttttcagg gtctggctca 5220gcagctccag gcggtcccag agctcggtga cgtgctctac ggcatctcta tccagcatat 5280ctcctcgttt cgcgggttgg ggcgactttc gctgtagggc accaagcggt ggtcgtccag 5340cggggccaga gtcatgtcct tccatgggcg cagggtcctc gtcagggtgg tctgggtcac 5400ggtgaagggg tgcgctccgg gctgagcgct tgccaaggtg cgcttgaggc tggttctgct 5460ggtgctgaag cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt 5520gtcatagtcc agcccctccg cggcgtgtcc cttggcgcgc agcttgccct tggaggtggc 5580gccgcacgag gggcagagca ggctcttgag cgcgtagagc ttgggggcga ggaagaccga 5640ttcgggggag taggcgtccg cgccgcagac cccgcacacg gtctcgcact ccaccagcca 5700ggtgagctcg gggcgcgccg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt 5760cttacctcgg gtctccatga ggtggtgtcc ccgctcggtg acgaagaggc tgtccgtgtc 5820tccgtagacc gacttgaggg gtcttttctc caggggggtc cctcggtctt cctcgtagag 5880gaactcggac cactctgaga cgaaggcccg cgtccaggcc aggacgaagg aggctatgtg 5940ggaggggtag cggtcgttgt ccactagggg gtccaccttc tccaaggtgt gaagacacat 6000gtcgccttcc tcggcgtcca ggaaggtgat tggcttgtag gtgtaggcca cgtgaccggg 6060ggttcctgac gggggggtat aaaagggggt gggggcgcgc tcgtcgtcac tctcttccgc 6120atcgctgtct gcgagggcca gctgctgggg tgagtattcc ctctcgaagg cgggcatgac 6180ctccgcgctg aggttgtcag tttccaaaaa cgaggaggat ttgatgttca cctgtcccga 6240ggtgatacct ttgagggtac ccgcgtccat ctggtcagaa aacacgatct ttttattgtc 6300cagcttggtg gcgaacgacc cgtagagggc gttggagagc agcttggcga tggagcgcag 6360ggtctggttc ttgtccctgt cggcgcgctc cttggccgcg atgttgagct gcacgtactc 6420gcgcgcgacg cagcgccact cggggaagac ggtggtgcgc tcgtcgggca ccaggcgcac 6480gcgccagccg cggttgtgca gggtgaccag gtccacgctg gtggcgacct cgccgcgcag 6540gcgctcgttg gtccagcaga gacggccgcc cttgcgcgag cagaaggggg gcagggggtc 6600gagctgggtc tcgtccgggg ggtccgcgtc cacggtgaaa accccggggc gcaggcgcgc 6660gtcgaagtag tctatcttgc aaccttgcat gtccagcgcc tgctgccagt cgcgggcggc 6720gagcgcgcgc tcgtaggggt tgagcggcgg gccccagggc atggggtggg tgagtgcgga 6780ggcgtacatg ccgcagatgt catagacgta gaggggctcc cgcaggaccc cgatgtaggt 6840ggggtagcag cggccgccgc ggatgctggc gcgcacgtag tcatacagct cgtgcgaggg 6900ggcgaggagg tcggggccca ggttggtgcg ggcggggcgc tccgcgcgga agacgatctg 6960cctgaagatg gcatgcgagt tggaagagat ggtggggcgc tggaagacgt tgaagctggc 7020gtcctgcagg ccgacggcgt cgcgcacgaa ggaggcgtag gagtcgcgca gcttgtgtac 7080cagctcggcg gtgacctgca cgtcgagcgc gcagtagtcg agggtctcgc ggatgatgtc 7140atatttagcc tgccccttct ttttccacag ctcgcggttg aggacaaact cttcgcggtc 7200tttccagtac tcttggatcg ggaaaccgtc cggttccgaa cggtaagagc ctagcatgta 7260gaactggttg acggcctggt aggcgcagca gcccttctcc acggggaggg cgtaggcctg 7320cgcggccttg cggagcgagg tgtgggtcag ggcgaaggtg tccctgacca tgactttgag 7380gtactggtgc ttgaagtcgg agtcgtcgca gccgccccgc tcccagagcg agaagtcggt 7440gcgcttcttg gagcgggggt tgggcagagc gaaggtgaca tcgttgaaga ggattttgcc 7500cgcgcggggc atgaagttgc gggtgatgcg gaagggcccc ggcacttcag agcggttgtt 7560gatgacctgg gcggcgagca cgatctcgtc gaagccgttg atgttgtggc ccacgatgta 7620gagttccagg aagcggggcc ggccctttac ggtgggcagc ttctttagct cttcgtaggt 7680gagctcctcg ggcgaggcga ggccgtgctc ggccagggcc cagtccgcga ggtgcgggtt 7740gtctctgagg aaggacttcc agaggtcgcg ggccaggagg gtctgcaggc ggtctctgaa 7800ggtcctgaac tggcggccca cggccatttt ttcgggggtg atgcagtaga aggtgagggg 7860gtcttgctgc cagcggtccc agtcgagctg cagggcgagg tcgcgcgcgg cggtgaccag 7920gcgctcgtcg cccccgaatt tcatgaccag catgaagggc acgagctgct ttccgaaggc 7980ccccatccaa gtgtaggtct ctacatcgta ggtgacaaag aggcgctccg tgcgaggatg 8040cgagccgatc gggaagaact ggatctcccg ccaccagttg gaggagtggc tgttgatgtg 8100gtggaagtag aagtcccgtc gccgggccga acactcgtgc tggcttttgt aaaagcgagc 8160gcagtactgg cagcgctgca cgggctgtac ctcatgcacg agatgcacct ttcgcccgcg 8220cacgaggaag ccgaggggaa atctgagccc cccgcctggc tcgcggcatg gctggttctc 8280ttctactttg gatgcgtgtc cgtctccgtc tggctcctcg aggggtgtta cggtggagcg 8340gaccaccacg ccgcgcgagc cgcaggtcca gatatcggcg cgcggcggtc ggagtttgat 8400gacgacatcg cgcagctggg agctgtccat ggtctggagc tcccgcggcg gcggcaggtc 8460agccgggagt tcttgcaggt tcacctcgca gagtcgggcc agggcgcggg gcaggtctag 8520gtggtacctg atctctaggg gcgtgttggt ggcggcgtcg atggcttgca ggagcccgca 8580gccccggggg gcgacgacgg tgccccgcgg ggtggtggtg gtggtggcgg tgcagctcag 8640aagcggtgcc gcgggcgggc ccccggaggt agggggggct ccggtcccgc gggcaggggc 8700ggcagcggca cgtcggcgtg gagcgcgggc aggagttggt gctgtgcccg gaggttgctg 8760gcgaaggcga cgacgcggcg gttgatctcc tggatctggc gcctctgcgt gaagacgacg 8820ggcccggtga gcttgaacct gaaagagagt tcgacagaat caatctcggt gtcattgacc 8880gcggcctggc gcaggatctc ctgcacgtct cccgagttgt cttggtaggc gatctcggcc 8940atgaactgct cgatctcttc ctcctggagg tctccgcgtc cggcgcgttc cacggtggcc 9000gccaggtcgt tggagatgcg ccccatgagc tgcgagaagg cgttgagtcc gccctcgttc 9060cagactcggc tgtagaccac gcccccctgg tcatcgcggg cgcgcatgac cacctgcgcg 9120aggttgagct ccacgtgccg cgcgaagacg gcgtagttgc gcagacgctg gaagaggtag 9180ttgagggtgg tggcggtgtg ctcggccacg aagaagttca tgacccagcg gcgcaacgtg 9240gattcgttga tgtcccccaa ggcctccagc cgttccatgg cctcgtagaa gtccacggcg 9300aagttgaaaa actgggagtt gcgcgccgac acggtcaact cctcctccag aagacggatg 9360agctcggcga cggtgtcgcg cacctcgcgc tcgaaggcta tggggatctc ttcctccgct 9420agcatcacca cctcctcctc ttcctcctct tctggcactt ccatgatggc ttcctcctct 9480tcggggggtg gcggcggcgg cggtggggga gggggcgctc tgcgccggcg gcggcgcacc 9540gggaggcggt ccacgaagcg cgcgatcatc tccccgcggc ggcggcgcat ggtctcggtg 9600acggcgcggc cgttctcccg ggggcgcagt tggaagacgc cgccggacat ctggtgctgg 9660ggcgggtggc cgtgaggcag cgagacggcg ctgacgatgc atctcaacaa ttgctgcgta 9720ggtacgccgc cgagggacct gagggagtcc atatccaccg gatccgaaaa cctttcgagg 9780aaggcgtcta accagtcgca gtcgcaaggt aggctgagca ccgtggcggg cggcgggggg 9840tggggggagt gtctggcgga ggtgctgctg atgatgtaat tgaagtaggc ggacttgaca 9900cggcggatgg tcgacaggag caccatgtcc ttgggtccgg cctgctggat gcggaggcgg 9960tcggctatgc cccaggcttc gttctggcat cggcgcaggt ccttgtagta gtcttgcatg 10020agcctttcca ccggcacctc ttctccttcc tcttctgctt cttccatgtc tgcttcggcc 10080ctggggcggc gccgcgcccc cctgcccccc atgcgcgtga ccccgaaccc cctgagcggt 10140tggagcaggg ccaggtcggc gacgacgcgc tcggccagga tggcctgctg cacctgcgtg 10200agggtggttt ggaagtcatc caagtccacg aagcggtggt aggcgcccgt gttgatggtg 10260taggtgcagt tggccatgac ggaccagttg acggtctggt ggcccggttg cgacatctcg 10320gtgtacctga gtcgcgagta ggcgcgggag tcgaagacgt agtcgttgca agtccgcacc 10380aggtactggt agcccaccag gaagtgcggc ggcggctggc ggtagagggg ccagcgcagg 10440gtggcggggg ctccgggggc caggtcttcc agcatgaggc ggtggtaggc gtagatgtac 10500ctggacatcc aggtgatacc cgcggcggtg gtggaggcgc gcgggaagtc gcgcacccgg 10560ttccagatgt tgcgcagggg cagaaagtgc tccatggtag gcgtgctctg tccagtcaga 10620cgcgcgcagt cgttgatact ctagaccagg gaaaacgaaa gccggtcagc gggcactctt 10680ccgtggtctg gtgaatagat cgcaagggta tcatggcgga gggcctcggt tcgagccccg 10740ggtccgggcc ggacggtccg ccatgatcca cgcggttacc gcccgcgtgt cgaacccagg 10800tgtgcgacgt cagacaacgg tggagtgttc cttttggcgt ttttctggcc gggcgccggc 10860gccgcgtaag agactaagcc gcgaaagcga aagcagtaag tggctcgctc cccgtagccg 10920gagggatcct tgctaagggt tgcgttgcgg cgaaccccgg ttcgaatccc gtactcgggc 10980cggccggacc cgcggctaag gtgttggatt ggcctccccc tcgtataaag accccgcttg 11040cggattgact ccggacacgg ggacgagccc cttttatttt tgctttcccc agatgcatcc 11100ggtgctgcgg cagatgcgcc ccccgcccca gcagcagcaa caacaccagc aagagcggca 11160gcaacagcag cgggagtcat gcagggcccc ctcacccacc ctcggcgggc cggccacctc 11220ggcgtccgcg gccgtgtctg gcgcctgcgg cggcggcggg gggccggctg acgaccccga 11280ggagcccccg cggcgcaggg ccagacacta cctggacctg gaggagggcg agggcctggc 11340gcggctgggg gcgccgtctc ccgagcgcca cccgcgggtg cagctgaagc gcgactcgcg 11400cgaggcgtac gtgcctcggc agaacctgtt cagggaccgc gcgggcgagg agcccgagga 11460gatgcgggac aggaggttca gcgcagggcg ggagctgcgg caggggctga accgcgagcg 11520gctgctgcgc gaggaggact ttgagcccga cgcgcggacg gggatcagcc ccgcgcgcgc 11580gcacgtggcg gccgccgacc tggtgacggc gtacgagcag acggtgaacc aggagatcaa 11640cttccaaaag agtttcaaca accacgtgcg cacgctggtg gcgcgcgagg aggtgaccat 11700cgggctgatg cacctgtggg actttgtaag cgcgctggtg cagaacccca acagcaagcc 11760tctgacggcg cagctgttcc tgatagtgca gcacagcagg gacaacgagg cgtttaggga 11820cgcgctgctg aacatcaccg agcccgaggg tcggtggctg ctggacctga ttaacatcct 11880gcagagcata gtggtgcagg agcgcagcct gagcctggcc gacaaggtgg cggccatcaa 11940ctactcgatg ctgagcctgg gcaagtttta cgcgcgcaag atctaccaga cgccgtacgt 12000gcccatagac aaggaggtga agatcgacgg tttttacatg cgcatggcgc tgaaggtgct 12060caccctgagc gacgacctgg gcgtgtaccg caacgagcgc atccacaagg ccgtgagcgt 12120gagccggcgg cgcgagctga gcgaccgcga gctgatgcac agcctgcagc gggcgctggc 12180gggcgccggc agcggcgaca gggaggcgga gtcctacttc gatgcggggg cggacctgcg 12240ctgggcgccc agccggcggg ccctggaggc cgcgggggtc cgcgaggact atgacgagga 12300cggcgaggag gatgaggagt acgagctaga ggagggcgag tacctggact aaaccgcggg 12360tggtgtttcc ggtagatgca agacccgaac gtggtggacc cggcgctgcg ggcggctctg 12420cagagccagc cgtccggcct taactcctca gacgactggc gacaggtcat ggaccgcatc 12480atgtcgctga cggcgcgtaa cccggacgcg ttccggcagc agccgcaggc caacaggctc 12540tccgccatcc tggaggcggt ggtgcctgcg cgctcgaacc ccacgcacga gaaggtgctg 12600gccatagtga acgcgctggc cgagaacagg gccatccgcc cggacgaggc cgggctggtg 12660tacgacgcgc tgctgcagcg cgtggcccgc tacaacagcg gcaacgtgca gaccaacctg 12720gaccggctgg tgggggacgt gcgcgaggcg gtggcgcagc gcgagcgcgc ggatcggcag 12780ggcaacctgg gctccatggt ggcgctgaat gccttcctga gcacgcagcc ggccaacgtg 12840ccgcgggggc aggaagacta caccaacttt gtgagcgcgc tgcggctgat ggtgaccgag 12900accccccaga gcgaggtgta ccagtcgggc ccggactact tcttccagac cagcagacag 12960ggcctgcaga cggtgaacct gagccaggct ttcaagaacc tgcgggggct gtggggcgtg 13020aaggcgccca ccggcgaccg ggcgacggtg tccagcctgc tgacgcccaa ctcgcgcctg 13080ctgctgctgc tgatcgcgcc gttcacggac agcggcagcg tgtcccggga cacctacctg 13140gggcacctgc tgaccctgta ccgcgaggcc atcgggcagg cgcaggtgga cgagcacacc 13200ttccaggaga tcaccagcgt gagccgcgcg ctggggcagg aggacacgag cagcctggag 13260gcgactctga actacctgct gaccaaccgg cggcagaaga ttccctcgct gcacagcctg 13320acctccgagg aggagcgcat cttgcgctac gtgcagcaga gcgtgagcct gaacctgatg 13380cgcgacgggg tgacgcccag cgtggcgctg gacatgaccg cgcgcaacat ggaaccgggc 13440atgtacgccg cgcaccggcc ttacatcaac cgcctgatgg actacctgca tcgcgcggcg 13500gccgtgaacc ccgagtactt taccaacgcc atcctgaacc cgcactggct cccgccgccc 13560gggttctaca gcgggggctt cgaggtcccg gagaccaacg atggcttcct gtgggacgac 13620atggacgaca gcgtgttctc cccgcggccg caggcgctgg cggaagcgtc cctgctgcgt 13680cccaagaagg aggaggagga ggaggcgagt cgccgccgcg gcagcagcgg cgtggcttct 13740ctgtccgagc tgggggcggc agccgccgcg cgccccgggt ccctgggcgg cagccccttt 13800ccgagcctgg tggggtctct gcacagcgag cgcaccaccc gccctcggct gctgggcgag 13860gacgagtacc tgaataactc cctgctgcag ccggtgcggg agaaaaacct gcctcccgcc 13920ttccccaaca acgggataga gagcctggtg gacaagatga gcagatggaa gacctatgcg 13980caggagcaca gggacgcgcc tgcgctccgg ccgcccacgc ggcgccagcg ccacgaccgg 14040cagcgggggc tggtgtggga tgacgaggac tccgcggacg atagcagcgt gctggacctg 14100ggagggagcg gcaacccgtt cgcgcacctg cgcccccgcc tggggaggat gttttaaaaa 14160aaaaaaaaaa aagcaagaag catgatgcaa aaattaaata aaactcacca aggccatggc 14220gaccgagcgt tggtttcttg tgttcccttc agtatgcggc gcgcggcgat gtaccaggag 14280ggacctcctc cctcttacga gagcgtggtg ggcgcggcgg cggcggcgcc ctcttctccc 14340tttgcgtcgc agctgctgga gccgccgtac gtgcctccgc gctacctgcg gcctacgggg 14400gggagaaaca gcatccgtta ctcggagctg gcgcccctgt tcgacaccac ccgggtgtac 14460ctggtggaca acaagtcggc ggacgtggcc tccctgaact accagaacga ccacagcaat 14520tttttgacca cggtcatcca gaacaatgac tacagcccga gcgaggccag cacccagacc 14580atcaatctgg atgaccggtc gcactggggc ggcgacctga aaaccatcct gcacaccaac 14640atgcccaacg tgaacgagtt catgttcacc aataagttca aggcgcgggt gatggtgtcg 14700cgctcgcaca ccaaggaaga ccgggtggag ctgaagtacg agtgggtgga gttcgagctg 14760ccagagggca actactccga gaccatgacc attgacctga tgaacaacgc gatcgtggag 14820cactatctga aagtgggcag gcagaacggg gtcctggaga gcgacatcgg ggtcaagttc 14880gacaccagga acttccgcct ggggctggac cccgtgaccg ggctggttat gcccggggtg 14940tacaccaacg aggccttcca tcccgacatc atcctgctgc ccggctgcgg ggtggacttc 15000acttacagcc gcctgagcaa cctcctgggc

atccgcaagc ggcagccctt ccaggagggc 15060ttcaggatca cctacgagga cctggagggg ggcaacatcc ccgcgctcct cgatgtggag 15120gcctaccagg atagcttgaa ggaaaatgag gcgggacagg aggataccgc ccccgccgcc 15180tccgccgccg ccgagcaggg cgaggatgct gctgacaccg cggccgcgga cggggcagag 15240gccgaccccg ctatggtggt ggaggctccc gagcaggagg aggacatgaa tgacagtgcg 15300gtgcgcggag acaccttcgt cacccggggg gaggaaaagc aagcggaggc cgaggccgcg 15360gccgaggaaa agcaactggc ggcagcagcg gcggcggcgg cgttggccgc ggcggaggct 15420gagtctgagg ggaccaagcc cgccaaggag cccgtgatta agcccctgac cgaagatagc 15480aagaagcgca gttacaacct gctcaaggac agcaccaaca ccgcgtaccg cagctggtac 15540ctggcctaca actacggcga cccgtcgacg ggggtgcgct cctggaccct gctgtgcacg 15600ccggacgtga cctgcggctc ggagcaggtg tactggtcgc tgcccgacat gatgcaagac 15660cccgtgacct tccgctccac gcggcaggtc agcaacttcc cggtggtggg cgccgagctg 15720ctgcccgtgc actccaagag cttctacaac gaccaggccg tctactccca gctcatccgc 15780cagttcacct ctctgaccca cgtgttcaat cgctttcctg agaaccagat tctggcgcgc 15840ccgcccgccc ccaccatcac caccgtcagt gaaaacgttc ctgctctcac agatcacggg 15900acgctaccgc tgcgcaacag catcggagga gtccagcgag tgaccgttac tgacgccaga 15960cgccgcacct gcccctacgt ttacaaggcc ttgggcatag tctcgccgcg cgtcctttcc 16020agccgcactt tttgagcaac accaccatca tgtccatcct gatctcaccc agcaataact 16080ccggctgggg actgctgcgc gcgcccagca agatgttcgg aggggcgagg aagcgttccg 16140agcagcaccc cgtgcgcgtg cgcgggcact tccgcgcccc ctggggagcg cacaaacgcg 16200gccgcgcggg gcgcaccacc gtggacgacg ccatcgactc ggtggtggag caggcgcgca 16260actacaggcc cgcggtctct accgtggacg cggccatcca gaccgtggtg cggggcgcgc 16320ggcggtacgc caagctgaag agccgccgga agcgcgtggc ccgccgccac cgccgccgac 16380ccggggccgc cgccaaacgc gccgccgcgg ccctgcttcg ccgggccaag cgcacgggcc 16440gccgcgccgc catgagggcc gcgcgccgct tggccgccgg catcaccgcc gccaccatgg 16500ccccccgtac ccgaagacgc gcggccgccg ccgccgccgc cgccatcagt gacatggcca 16560gcaggcgccg gggcaacgtg tactgggtgc gcgactcggt gaccggcacg cgcgtgcccg 16620tgcgcttccg ccccccgcgg acttgagatg atgtgaaaaa acaacactga gtctcctgct 16680gttgtgtgta tcccagcggc ggcggcgcgc gcagcgtcat gtccaagcgc aaaatcaaag 16740aagagatgct ccaggtcgtc gcgccggaga tctatgggcc cccgaagaag gaagagcagg 16800attcgaagcc ccgcaagata aagcgggtca aaaagaaaaa gaaagatgat gacgatgccg 16860atggggaggt ggagttcctg cgcgccacgg cgcccaggcg cccggtgcag tggaagggcc 16920ggcgcgtaaa gcgcgtcctg cgccccggca ccgcggtggt cttcacgccc ggcgagcgct 16980ccacccggac tttcaagcgc gtctatgacg aggtgtacgg cgacgaagac ctgctggagc 17040aggccaacga gcgcttcgga gagtttgctt acgggaagcg tcagcgggcg ctggggaagg 17100aggacctgct ggcgctgccg ctggaccagg gcaaccccac ccccagtctg aagcccgtga 17160ccctgcagca ggtgctgccg agcagcgcac cctccgaggc gaagcggggt ctgaagcgcg 17220agggcggcga cctggcgccc accgtgcagc tcatggtgcc caagcggcag aggctggagg 17280atgtgctgga gaaaatgaaa gtagaccccg gtctgcagcc ggacatcagg gtccgcccca 17340tcaagcaggt ggcgccgggc ctcggcgtgc agaccgtgga cgtggtcatc cccaccggca 17400actcccccgc cgccgccacc actaccgctg cctccacgga catggagaca cagaccgatc 17460ccgccgcagc cgcagccgca gccgccgccg cgacctcctc ggcggaggtg cagacggacc 17520cctggctgcc gccggcgatg tcagctcccc gcgcgcgtcg cgggcgcagg aagtacggcg 17580ccgccaacgc gctcctgccc gagtacgcct tgcatccttc catcgcgccc acccccggct 17640accgaggcta tacctaccgc ccgcgaagag ccaagggttc cacccgccgt ccccgccgac 17700gcgccgccgc caccacccgc cgccgccgcc gcagacgcca gcccgcactg gctccagtct 17760ccgtgaggaa agtggcgcgc gacggacaca ccctggtgct gcccagggcg cgctaccacc 17820ccagcatcgt ttaaaagcct gttgtggttc ttgcagatat ggccctcact tgccgcctcc 17880gtttcccggt gccgggatac cgaggaggaa gatcgcgccg caggaggggt ctggccggcc 17940gcggcctgag cggaggcagc cgccgcgcgc accggcggcg acgcgccacc agccgacgca 18000tgcgcggcgg ggtgctgccc ctgttaatcc ccctgatcgc cgcggcgatc ggcgccgtgc 18060ccgggatcgc ctccgtggcc ttgcaagcgt cccagaggca ttgacagact tgcaaacttg 18120caaatatgga aaaaaaaacc ccaataaaaa agtctagact ctcacgctcg cttggtcctg 18180tgactatttt gtagaatgga agacatcaac tttgcgtcgc tggccccgcg tcacggctcg 18240cgcccgttcc tgggacactg gaacgatatc ggcaccagca acatgagcgg tggcgccttc 18300agttggggct ctctgtggag cggcattaaa agtatcgggt ctgccgttaa aaattacggc 18360tcccgggcct ggaacagcag cacgggccag atgttgagag acaagttgaa agagcagaac 18420ttccagcaga aggtggtgga gggcctggcc tccggcatca acggggtggt ggacctggcc 18480aaccaggccg tgcagaataa gatcaacagc agactggacc cccggccgcc ggtggaggag 18540gtgccgccgg cgctggagac ggtgtccccc gatgggcgtg gcgagaagcg cccgcggccc 18600gatagggaag agaccactct ggtcacgcag accgatgagc cgcccccgta tgaggaggcc 18660ctgaagcaag gtctgcccac cacgcggccc atcgcgccca tggccaccgg ggtggtgggc 18720cgccacaccc ccgccacgct ggacttgcct ccgcccgccg atgtgccgca gcagcagaag 18780gcggcacagc cgggcccgcc cgcgaccgcc tcccgttcct ccgccggtcc tctgcgccgc 18840gcggccagcg gcccccgcgg gggggtcgcg aggcacggca actggcagag cacgctgaac 18900agcatcgtgg gtctgggggt gcggtccgtg aagcgccgcc gatgctactg aatagcttag 18960ctaacgtgtt gtatgtgtgt atgcgcccta tgtcgccgcc agaggagctg ctgagtcgcc 19020gccgttcgcg cgcccaccac caccgccact ccgcccctca agatggcgac cccatcgatg 19080atgccgcagt ggtcgtacat gcacatctcg ggccaggacg cctcggagta cctgagcccc 19140gggctggtgc agttcgcccg cgccaccgag agctacttca gcctgagtaa caagtttagg 19200aaccccacgg tggcgcccac gcacgatgtg accaccgacc ggtctcagcg cctgacgctg 19260cggttcattc ccgtggaccg cgaggacacc gcgtactcgt acaaggcgcg gttcaccctg 19320gccgtgggcg acaaccgcgt gctggacatg gcctccacct actttgacat ccgcggggtg 19380ctggaccggg gtcccacttt caagccctac tctggcaccg cctacaactc cctggccccc 19440aagggcgctc ccaactcctg cgagtgggag caagaggaaa ctcaggcagt tgaagaagca 19500gcagaagagg aagaagaaga tgctgacggt caagctgagg aagagcaagc agctaccaaa 19560aagactcatg tatatgctca ggctcccctt tctggcgaaa aaattagtaa agatggtctg 19620caaataggaa cggacgctac agctacagaa caaaaaccta tttatgcaga ccctacattc 19680cagcccgaac cccaaatcgg ggagtcccag tggaatgagg cagatgctac agtcgccggc 19740ggtagagtgc taaagaaatc tactcccatg aaaccatgct atggttccta tgcaagaccc 19800acaaatgcta atggaggtca gggtgtacta acggcaaatg cccagggaca gctagaatct 19860caggttgaaa tgcaattctt ttcaacttct gaaaacgccc gtaacgaggc taacaacatt 19920cagcccaaat tggtgctgta tagtgaggat gtgcacatgg agaccccgga tacgcacctt 19980tcttacaagc ccgcaaaaag cgatgacaat tcaaaaatca tgctgggtca gcagtccatg 20040cccaacagac ctaattacat cggcttcaga gacaacttta tcggcctcat gtattacaat 20100agcactggca acatgggagt gcttgcaggt caggcctctc agttgaatgc agtggtggac 20160ttgcaagaca gaaacacaga actgtcctac cagctcttgc ttgattccat gggtgacaga 20220accagatact tttccatgtg gaatcaggca gtggacagtt atgacccaga tgttagaatt 20280attgaaaatc atggaactga agacgagctc cccaactatt gtttccctct gggtggcata 20340ggggtaactg acacttacca ggctgttaaa accaacaatg gcaataacgg gggccaggtg 20400acttggacaa aagatgaaac ttttgcagat cgcaatgaaa taggggtggg aaacaatttc 20460gctatggaga tcaacctcag tgccaacctg tggagaaact tcctgtactc caacgtggcg 20520ctgtacctac cagacaagct taagtacaac ccctccaatg tggacatctc tgacaacccc 20580aacacctacg attacatgaa caagcgagtg gtggccccgg ggctggtgga ctgctacatc 20640aacctgggcg cgcgctggtc gctggactac atggacaacg tcaacccctt caaccaccac 20700cgcaatgcgg gcctgcgcta ccgctccatg ctcctgggca acgggcgcta cgtgcccttc 20760cacatccagg tgccccagaa gttctttgcc atcaagaacc tcctcctcct gccgggctcc 20820tacacctacg agtggaactt caggaaggat gtcaacatgg tcctccagag ctctctgggt 20880aacgatctca gggtggacgg ggccagcatc aagttcgaga gcatctgcct ctacgccacc 20940ttcttcccca tggcccacaa cacggcctcc acgctcgagg ccatgctcag gaacgacacc 21000aacgaccagt ccttcaatga ctacctctcc gccgccaaca tgctctaccc catacccgcc 21060aacgccacca acgtccccat ctccatcccc tcgcgcaact gggcggcctt ccgcggctgg 21120gccttcaccc gcctcaagac caaggagacc ccctccctgg gctcgggatt cgacccctac 21180tacacctact cgggctccat tccctacctg gacggcacct tctacctcaa ccacactttc 21240aagaaggtct cggtcacctt cgactcctcg gtcagctggc cgggcaacga ccgtctgctc 21300acccccaacg agttcgagat caagcgctcg gtcgacgggg agggctacaa cgtggcccag 21360tgcaacatga ccaaggactg gttcctggtc cagatgctgg ccaactacaa catcggctac 21420cagggcttct acatcccaga gagctacaag gacaggatgt actccttctt caggaacttc 21480cagcccatga gccggcaggt ggtggaccag accaagtaca aggactacca ggaggtgggc 21540atcatccacc agcacaacaa ctcgggcttc gtgggctacc tcgcccccac catgcgcgag 21600ggacaggcct accccgccaa cttcccctat ccgctcatag gcaagaccgc ggtcgacagc 21660atcacccaga aaaagttcct ctgcgaccgc accctctggc gcatcccctt ctccagcaac 21720ttcatgtcca tgggtgcgct ctcggacctg ggccagaact tgctctacgc caactccgcc 21780cacgccctcg acatgacctt cgaggtcgac cccatggacg agcccaccct tctctatgtt 21840ctgttcgaag tctttgacgt ggtccgggtc caccagccgc accgcggcgt catcgagacc 21900gtgtacctgc gtacgccctt ctcggccggc aacgccacca cctaaagaag caagccgcag 21960tcatcgccgc ctgcatgccg tcgggttcca ccgagcaaga gctcagggcc atcgtcagag 22020acctgggatg cgggccctat tttttgggca ccttcgacaa gcgcttccct ggctttgtct 22080ccccacacaa gctggcctgc gccatcgtca acacggccgg ccgcgagacc gggggcgtgc 22140actggctggc cttcgcctgg aacccgcgct ccaaaacatg cttcctcttt gaccccttcg 22200gcttttcgga ccagcggctc aagcaaatct acgagttcga gtacgagggc ttgctgcgtc 22260gcagcgccat cgcctcctcg cccgaccgct gcgtcaccct cgaaaagtcc acccagaccg 22320tgcaggggcc cgactcggcc gcctgcggtc tcttctgctg catgtttctg cacgcctttg 22380tgcactggcc tcagagtccc atggaccgca accccaccat gaacttgctg acgggggtgc 22440ccaactccat gctccagagc ccccaggtcg agcccaccct gcgccgcaac caggagcagc 22500tctacagctt cctggagcgc cactcgcctt acttccgccg ccacagcgca cagatcagga 22560gggccacctc cttctgccac ttgcaagaga tgcaagaagg gtaataacga tgtacacact 22620ttttttctca ataaatggca tctttttatt tatacaagct ctctggggta ttcatttccc 22680accaccaccc gccgttgtcg ccatctggct ctatttagaa atcgaaaggg ttctgccggg 22740agtcgccgtg cgccacgggc agggacacgt tgcgatactg gtagcgggtg ccccacttga 22800actcgggcac caccaggcga ggcagctcgg ggaagttttc gctccacagg ctgcgggtca 22860gcaccagcgc gttcatcagg tcgggcgccg agatcttgaa gtcgcagttg gggccgccgc 22920cctgcgcgcg cgagttgcgg tacaccgggt tgcagcactg gaacaccaac agcgccgggt 22980gcttcacgct ggccagcacg ctgcggtcgg agatcagctc ggcgtccagg tcctccgcgt 23040tgctcagcgc gaacggggtc atcttgggca cttgccgccc caggaagggc gcgtgccccg 23100gtttcgagtt gcagtcgcag cgcagcggga tcagcaggtg cccgtgcccg gactcggcgt 23160tggggtacag cgcgcgcatg aaggcctgca tctggcggaa ggccatctgg gccttggcgc 23220cctccgagaa gaacatgccg caggacttgc ccgagaactg gtttgcgggg cagctggcgt 23280cgtgcaggca gcagcgcgcg tcggtgttgg cgatctgcac cacgttgcgc ccccaccggt 23340tcttcacgat cttggccttg gacgattgct ccttcagcgc gcgctgcccg ttctcgctgg 23400tcacatccat ctcgatcaca tgttccttgt tcaccatgct gctgccgtgc agacacttca 23460gctcgccctc cgtctcggtg cagcggtgct gccacagcgc gcagcccgtg ggctcgaaag 23520acttgtaggt cacctccgcg aaggactgca ggtacccctg caaaaagcgg cccatcatgg 23580tcacgaaggt cttgttgctg ctgaaggtca gctgcagccc gcggtgctcc tcgttcagcc 23640aggtcttgca cacggccgcc agcgcctcca cctggtcggg cagcatcttg aagttcacct 23700tcagctcatt ctccacgtgg tacttgtcca tcagcgtgcg cgccgcctcc atgcccttct 23760cccaggccga caccagcggc aggctcacgg ggttcttcac catcaccgtg gccgccgcct 23820ccgccgcgct ttcgctttcc gccccgctgt tctcttcctc ttcctcctct tcctcgccgc 23880cgcccactcg cagcccccgc accacggggt cgtcttcctg caggcgctgc accttgcgct 23940tgccgttgcg cccctgcttg atgcgcacgg gcgggttgct gaagcccacc atcaccagcg 24000cggcctcttc ttgctcgtcc tcgctgtcca gaatgacctc cggggagggg gggttggtca 24060tcctcagtac cgaggcacgc ttctttttct tcctgggggc gttcgccagc tccgcggctg 24120cggccgctgc cgaggtcgaa ggccgagggc tgggcgtgcg cggcaccagc gcgtcctgcg 24180agccgtcctc gtcctcctcg gactcgagac ggaggcgggc ccgcttcttc gggggcgcgc 24240ggggcggcgg aggcggcggc ggcgacggag acggggacga gacatcgtcc agggtgggtg 24300gacggcgggc cgcgccgcgt ccgcgctcgg gggtggtctc gcgctggtcc tcttcccgac 24360tggccatctc ccactgctcc ttctcctata ggcagaaaga gatcatggag tctctcatgc 24420gagtcgagaa ggaggaggac agcctaaccg ccccctctga gccctccacc accgccgcca 24480ccaccgccaa tgccgccgcg gacgacgcgc ccaccgagac caccgccagt accaccctcc 24540ccagcgacgc acccccgctc gagaatgaag tgctgatcga gcaggacccg ggttttgtga 24600gcggagagga ggatgaggtg gatgagaagg agaaggagga ggtcgccgcc tcagtgccaa 24660aagaggataa aaagcaagac caggacgacg cagataagga tgagacagca gtcgggcggg 24720ggaacggaag ccatgatgct gatgacggct acctagacgt gggagacgac gtgctgctta 24780agcacctgca ccgccagtgc gtcatcgtct gcgacgcgct gcaggagcgc tgcgaagtgc 24840ccctggacgt ggcggaggtc agccgcgcct acgagcggca cctcttcgcg ccgcacgtgc 24900cccccaagcg ccgggagaac ggcacctgcg agcccaaccc gcgtctcaac ttctacccgg 24960tcttcgcggt acccgaggtg ctggccacct accacatctt tttccaaaac tgcaagatcc 25020ccctctcctg ccgcgccaac cgcacccgcg ccgacaaaac cctgaccctg cggcagggcg 25080cccacatacc tgatatcgcc tctctggagg aagtgcccaa gatcttcgag ggtctcggtc 25140gcgacgagaa acgggcggcg aacgctctgc acggagacag cgaaaacgag agtcactcgg 25200gggtgctggt ggagctcgag ggcgacaacg cgcgcctggc cgtactcaag cgcagcatag 25260aggtcaccca ctttgcctac ccggcgctca acctgccccc caaggtcatg agtgtggtca 25320tgggcgagct catcatgcgc cgcgcccagc ccctggccgc ggatgcaaac ttgcaagagt 25380cctccgagga aggcctgccc gcggtcagcg acgagcagct ggcgcgctgg ctggagaccc 25440gcgaccccgc gcagctggag gagcggcgca agctcatgat ggccgcggtg ctggtcaccg 25500tggagctcga gtgtctgcag cgcttcttcg cggaccccga gatgcagcgc aagctcgagg 25560agaccctgca ctacaccttc cgccagggct acgtgcgcca ggcctgcaag atctccaacg 25620tggagctctg caacctggtc tcctacctgg gcatcctgca cgagaaccgc ctcgggcaga 25680acgtcctgca ctccaccctc aaaggggagg cgcgccgcga ctacatccgc gactgcgcct 25740acctcttcct ctgctacacc tggcagacgg ccatgggggt ctggcagcag tgcctggagg 25800agcgcaacct caaggagctg gaaaagctcc tcaagcgcac cctcagggac ctctggacgg 25860gcttcaacga gcgctcggtg gccgccgcgc tggcggacat catctttccc gagcgcctgc 25920tcaagaccct gcagcagggc ctgcccgact tcaccagcca gagcatgctg cagaacttca 25980ggactttcat cctggagcgc tcgggcatcc tgccggccac ttgctgcgcg ctgcccagcg 26040acttcgtgcc catcaagtac agggagtgcc cgccgccgct ctggggccac tgctacctct 26100tccagctggc caactacctc gcctaccact cggacctcat ggaagacgtg agcggcgagg 26160gcctgctcga gtgccactgc cgctgcaacc tctgcacgcc ccaccgctct ctagtctgca 26220acccgcagct gctcagcgag agtcagatta tcggtacctt cgagctgcag ggtccctcgc 26280ctgacgagaa gtccgcggct ccagggctga aactcactcc ggggctgtgg acttccgcct 26340acctacgcaa atttgtacct gaggactacc acgcccacga gatcaggttc tacgaagacc 26400aatcccgccc gcccaaggcg gagctcaccg cctgcgtcat cacccagggg cacatcctgg 26460gccaattgca agccatcaac aaagcccgcc gagagttctt gctgaaaaag ggtcgggggg 26520tgtacctgga cccccagtcc ggcgaggagc taaacccgct acccccgccg ccgccccagc 26580agcgggacct tgcttcccag gatggcaccc agaaagaagc agcagccgcc gccgccgccg 26640cagccataca tgcttctgga ggaagaggag gaggactggg acagtcaggc agaggaggtt 26700tcggacgagg agcaggagga gatgatggaa gactgggagg aggacagcag cctagacgag 26760gaagcttcag aggccgaaga ggtggcagac gcaacaccat cgccctcggt cgcagccccc 26820tcgccggggc ccctgaaatc ctccgaaccc agcaccagcg ctataacctc cgctcctccg 26880gcgccggcgc cacccgcccg cagacccaac cgtagatggg acaccacagg aaccggggtc 26940ggtaagtcca agtgcccgcc gccgccaccg cagcagcagc agcagcagcg ccagggctac 27000cgctcgtggc gcgggcacaa gaacgccata gtcgcctgct tgcaagactg cgggggcaac 27060atctctttcg cccgccgctt cctgctattc caccacgggg tcgcctttcc ccgcaatgtc 27120ctgcattact accgtcatct ctacagcccc tactgcagcg gcgacccaga ggcggcagcg 27180gcagccacag cggcgaccac cacctaggaa gatatcctcc gcgggcaaga cagcggcagc 27240agcggccagg agacccgcgg cagcagcggc gggagcggtg ggcgcactgc gcctctcgcc 27300caacgaaccc ctctcgaccc gggagctcag acacaggatc ttccccactt tgtatgccat 27360cttccaacag agcagaggcc aggagcagga gctgaaaata aaaaacagat ctctgcgctc 27420cctcacccgc agctgtctgt atcacaaaag cgaagatcag cttcggcgca cgctggagga 27480cgcggaggca ctcttcagca aatactgcgc gctcactctt aaagactagc tccgcgccct 27540tctcgaattt aggcgggaga aaactacgtc atcgccggcc gccgcccagc ccgcccagcc 27600gagatgagca aagagattcc cacgccatac atgtggagct accagccgca gatgggactc 27660gcggcgggag cggcccagga ctactccacc cgcatgaact acatgagcgc gggaccccac 27720atgatctcac aggtcaacgg gatccgcgcc cagcgaaacc aaatactgct ggaacaggcg 27780gccatcaccg ccacgccccg ccataatctc aacccccgaa attggcccgc cgccctcgtg 27840taccaggaaa ccccctccgc caccaccgta ctacttccgc gtgacgccca ggccgaagtc 27900cagatgacta actcaggggc gcagctcgcg ggcggctttc gtcacggggc gcggccgctc 27960cgaccaggta taagacacct gatgatcaga ggccgaggta tccagctcaa cgacgagtcg 28020gtgagctctt cgctcggtct ccgtccggac ggaactttcc agctcgccgg atccggccgc 28080tcttcgttca cgccccgcca ggcgtacctg actctgcaga cctcgtcctc ggagccccgc 28140tccggcggca tcggaaccct ccagttcgtg gaggagttcg tgccctcggt ctacttcaac 28200cccttctcgg gacctcccgg acgctacccc gaccagttca ttccgaactt tgacgcggtg 28260aaggactcgg cggacggcta cgactgaatg tcaggtgtcg aggcagagca gcttcgcctg 28320agacacctcg agcactgccg ccgccacaag tgcttcgccc gcggttctgg tgagttctgc 28380tactttcagc tacccgagga gcataccgag gggccggcgc acggcgtccg cctgaccacc 28440cagggcgagg ttacctgttc cctcatccgg gagtttaccc tccgtcccct gctagtggag 28500cgggagcggg gtccctgtgt cctaactatc gcctgcaact gccctaaccc tggattacat 28560caagatcttt gctgtcatct ctgtgctgag tttaataaac gctgagatca gaatctactg 28620gggctcctgt cgccatcctg tgaacgccac cgtcttcacc caccccgacc aggcccaggc 28680gaacctcacc tgcggtctgc atcggagggc caagaagtac ctcacctggt acttcaacgg 28740cacccccttt gtggtttaca acagcttcga cggggacgga gtctccctga aagaccagct 28800ctccggtctc agctactcca tccacaagaa caccaccctc caactcttcc ctccctacct 28860gccgggaacc tacgagtgcg tcaccggccg ctgcacccac ctcacccgcc tgatcgtaaa 28920ccagagcttt ccgggaacag ataactccct cttccccaga acaggaggtg agctcaggaa 28980actccccggg gaccagggcg gagacgtacc ttcgaccctt gtggggttag gattttttat 29040taccgggttg ctggctcttt taatcaaagt ttccttgaga tttgttcttt ccttctacgt 29100gtatgaacac ctcaacctcc aataactcta ccctttcttc ggaatcaggt gacttctctg 29160aaatcgggct tggtgtgctg cttactctgt tgattttttt ccttatcata ctcagccttc 29220tgtgcctcag gctcgccgcc tgctgcgcac acatctatat ctactgctgg ttgctcaagt 29280gcaggggtcg ccacccaaga tgaacaggta catggtccta tcgatcctag gcctgctggc 29340cctggcggcc tgcagcgccg ccaaaaaaga gattaccttt gaggagcccg cttgcaatgt 29400aactttcaag cccgagggtg accaatgcac caccctcgtc aaatgcgtta ccaatcatga 29460gaggctgcgc atcgactaca aaaacaaaac tggccagttt gcggtctata gtgtgtttac 29520gcccggagac ccctctaact actctgtcac cgtcttccag ggcggacagt ctaagatatt 29580caattacact ttcccttttt atgagttatg cgatgcggtc atgtacatgt caaaacagta 29640caacctgtgg cctccctctc cccaggcgtg tgtggaaaat actgggtctt actgctgtat 29700ggctttcgca atcactacgc tcgctctaat ctgcacggtg ctatacataa aattcaggca 29760gaggcgaatc tttatcgatg aaaagaaaat gccttgatcg ctaacaccgg ctttctatct 29820gcagaatgaa tgcaatcacc tccctactaa tcaccaccac cctccttgcg attgcccatg 29880ggttgacacg aatcgaagtg ccagtggggt ccaatgtcac catggtgggc cccgccggca 29940attccaccct catgtgggaa aaatttgtcc gcaatcaatg ggttcatttc tgctctaacc 30000gaatcagtat caagcccaga gccatctgcg atgggcaaaa tctaactctg atcaatgtgc 30060aaatgatgga tgctgggtac tattacgggc

agcggggaga aatcattaat tactggcgac 30120cccacaagga ctacatgctg catgtagtcg aggcacttcc cactaccacc cccactacca 30180cctctcccac caccaccacc actactacta ctactactac tactactact actaccacta 30240ccgctgcccg ccatacccgc aaaagcacca tgattagcac aaagccccct cgtgctcact 30300cccacgccgg cgggcccatc ggtgcgacct cagaaaccac cgagctttgc ttctgccaat 30360gcactaacgc cagcgctcat gaactgttcg acctggagaa tgaggatgtc cagcagagct 30420ccgcttgcct gacccaggag gctgtggagc ccgttgccct gaagcagatc ggtgattcaa 30480taattgactc ttcttctttt gccactcccg aataccctcc cgattctact ttccacatca 30540cgggtaccaa agaccctaac ctctctttct acctgatgct gctgctctgt atctctgtgg 30600tctcttccgc gctgatgtta ctggggatgt tctgctgcct gatctgccgc agaaagagaa 30660aagctcgctc tcagggccaa ccactgatgc ccttccccta ccccccggat tttgcagata 30720acaagatatg agctcgctgc tgacactaac cgctttacta gcctgcgctc taacccttgt 30780cgcttgcgac tcgagattcc acaatgtcac agctgtggca ggagaaaatg ttactttcaa 30840ctccacggcc gatacccagt ggtcgtggag tggctcaggt agctacttaa ctatctgcaa 30900tagctccact tcccccggca tatccccaac caagtaccaa tgcaatgcca gcctgttcac 30960cctcatcaac gcttccaccc tggacaatgg actctatgta ggctatgtac cctttggtgg 31020gcaaggaaag acccacgctt acaacctgga agttcgccag cccagaacca ctacccaagc 31080ttctcccacc accaccacca ccaccaccat caccagcagc agcagcagca gcagccacag 31140cagcagcagc agattattga ctttggtttt ggccagctca tctgccgcta cccaggccat 31200ctacagctct gtgcccgaaa ccactcagat ccaccgccca gaaacgacca ccgccaccac 31260cctacacacc tccagcgatc agatgccgac caacatcacc cccttggctc ttcaaatggg 31320acttacaagc cccactccaa aaccagtgga tgcggccgag gtctccgccc tcgtcaatga 31380ctgggcgggg ctgggaatgt ggtggttcgc cataggcatg atggcgctct gcctgcttct 31440gctctggctc atctgctgcc tccaccgcag gcgagccaga ccccccatct atagacccat 31500cattgtcctg aaccccgata atgatgggat ccatagattg gatggcctga aaaacctact 31560tttttctttt acagtatgat aaattgagac atgcctcgca ttttcttgta catgttcctt 31620ctcccacctt ttctggggtg ttctacgctg gccgctgtgt ctcacctgga ggtagactgc 31680ctctcaccct tcactgtcta cctgctttac ggattggtca ccctcactct catctgcagc 31740ctaatcacag taatcatcgc cttcatccag tgcattgatt acatctgtgt gcgcctcgca 31800tacttcagac accacccgca gtaccgagac aggaacattg cccaacttct aagactgctc 31860taatcatgca taagactgtg atctgccttc tgatcctctg catcctgccc accctcacct 31920cctgccagta caccacaaaa tctccgcgca aaagacatgc ctcctgccgc ttcacccaac 31980tgtggaatat acccaaatgc tacaacgaaa agagcgagct ctccgaagct tggctgtatg 32040gggtcatctg tgtcttagtt ttctgcagca ctgtctttgc cctcataatc tacccctact 32100ttgatttggg atggaacgcg atcgatgcca tgaattaccc cacctttccc gcacccgaga 32160taattccact gcgacaagtt gtacccgttg tcgttaatca acgcccccca tcccctacgc 32220ccactgaaat cagctacttt aacctaacag gcggagatga ctgacgccct agatctagaa 32280atggacggca tcagtaccga gcagcgtctc ctagagaggc gcaggcaggc ggctgagcaa 32340gagcgcctca atcaggagct ccgagatctc gttaacctgc accagtgcaa aagaggcatc 32400ttttgtctgg taaagcaggc caaagtcacc tacgagaaga ccggcaacag ccaccgcctc 32460agttacaaat tgcccaccca gcgccagaag ctggtgctca tggtgggtga gaatcccatc 32520accgtcaccc agcactcggt agagaccgag gggtgtctgc actccccctg tcggggtcca 32580gaagacctct gcaccctggt aaagaccctg tgcggtctca gagatttagt cccctttaac 32640taatcaaaca ctggaatcaa taaaaagaat cacttactta aaatcagaca gcaggtctct 32700gtccagttta ttcagcagca cctccttccc ctcctcccaa ctctggtact ccaaacgcct 32760tctggcggca aacttcctcc acaccctgaa gggaatgtca gattcttgct cctgtccctc 32820cgcacccact atcttcatgt tgttgcagat gaagcgcacc aaaacgtctg acgagagctt 32880caaccccgtg tacccctatg acacggaaag cggccctccc tccgtccctt tcctcacccc 32940tcccttcgtg tctcccgatg gattccaaga aagtcccccc ggggtcctgt ctctgaacct 33000ggccgagccc ctggtcactt cccacggcat gctcgccctg aaaatgggaa gtggcctctc 33060cctggacgac gctggcaacc tcacctctca agatatcacc accgctagcc ctcccctcaa 33120aaaaaccaag accaacctca gcctagaaac ctcatccccc ctaactgtga gcacctcagg 33180cgccctcacc gtagcagccg ccgctcccct ggcggtggcc ggcacctccc tcaccatgca 33240atcagaggcc cccctgacag tacaggatgc aaaactcacc ctggccacca aaggccccct 33300gaccgtgtct gaaggcaaac tggccttgca aacatcggcc ccgctgacgg ccgctgacag 33360cagcaccctc acagtcagtg ccacaccacc ccttagcaca agcaatggca gcttgggtat 33420tgacatgcaa gcccccattt acaccaccaa tggaaaacta ggacttaact ttggcgctcc 33480cctgcatgtg gtagacagcc taaatgcact gactgtagtt actggccaag gtcttacgat 33540aaacggaaca gccctacaaa ctagagtctc aggtgccctc aactatgaca catcaggaaa 33600cctagaattg agagctgcag ggggtatgcg agttgatgca aatggtcaac ttatccttga 33660tgtagcttac ccatttgatg cacaaaacaa tctcagcctt aggcttggac agggacccct 33720gtttgttaac tctgcccaca acttggatgt taactacaac agaggcctct acctgttcac 33780atctggaaat accaaaaagc tagaagttaa tatcaaaaca gccaagggtc tcatttatga 33840tgacactgct atagcaatca atgcgggtga tgggctacag tttgactcag gctcagatac 33900aaatccatta aaaactaaac ttggattagg actggattat gactccagca gagccataat 33960tgctaaactg ggaactggcc taagctttga caacacaggt gccatcacag taggcaacaa 34020aaatgatgac aagcttacct tgtggaccac accagaccca tcccctaact gtagaatcta 34080ttcagagaaa gatgctaaat tcacacttgt tttgactaaa tgcggcagtc aggtgttggc 34140cagcgtttct gttttatctg taaaaggtag ccttgcgccc atcagtggca cagtaactag 34200tgctcagatt gtcctcagat ttgatgaaaa tggagttcta ctaagcaatt cttcccttga 34260ccctcaatac tggaactaca gaaaaggtga ccttacagag ggcactgcat ataccaacgc 34320agtgggattt atgcccaacc tcacagcata cccaaaaaca cagagccaaa ctgctaaaag 34380caacattgta agtcaggttt acttgaatgg ggacaaatcc aaacccatga ccctcaccat 34440taccctcaat ggaactaatg aaacaggaga tgccacagta agcacttact ccatgtcatt 34500ctcatggaac tggaatggaa gtaattacat taatgaaacg ttccaaacca actccttcac 34560cttctcctac atcgcccaag aataaaaagc atgacgctgt tgatttgatt caatgtgttt 34620ctgttttatt ttcaagcaca acaaaatcat tcaagtcatt cttccatctt agcttaatag 34680acacagtagc ttaatagacc cagtagtgca aagccccatt ctagcttata gatcagacag 34740tgataattaa ccaccaccac caccatacct tttgattcag gaaatcatga tcatcacagg 34800atcctagtct tcaggccgcc ccctccctcc caagacacag aatacacagt cctctccccc 34860cgactggctt taaataacac catctggttg gtcacagaca tgttcttagg ggttatattc 34920cacacggtct cctgccgcgc caggcgctcg tcggtgatgt tgataaactc tcccggcagc 34980tcgctcaagt tcacgtcgct gtccagcggc tgaacctccg gctgacgcga taactgtgcg 35040accggctgct ggacgaacgg aggccgcgcc tacaaggggg tagagtcata atcctcggtc 35100aggatagggc ggtgatgcag cagcagcgag cgaaacatct gctgccgccg ccgctccgtc 35160cggcaggaaa acaacacgcc ggtggtctcc tccgcgataa tccgcaccgc ccgcagcatc 35220agcttcctcg ttctccgcgc gcagcacctc acccttatct cgctcaaatc ggcgcagtag 35280gtacagcaca gcaccacgat gttattcatg atcccacagt gcagggcgct gtatccaaag 35340ctcatgccgg gaaccaccgc ccccacgtgg ccatcgtacc acaagcgcac gtaaatcaag 35400tgtcgacccc tcatgaacgc gctggacaca aacattactt ccttgggcat gttgtaattc 35460accacctccc ggtaccagat aaacctctgg ttgaacaggg caccttccac caccatcctg 35520aaccaagagg ccagaacctg cccaccggct atgcactgca gggaacccgg gttggaacaa 35580tgacaatgca gactccaagg ctcgtaaccg tggatcatcc ggctgctgaa ggcatcgatg 35640ttggcacaac acagacacac gtgcatgcac tttctcatga ttagcagctc ttccctcgtc 35700aggatcatat cccaaggaat aacccattct tgaatcaacg taaaacccac acagcaggga 35760aggcctcgca cataactcac gttgtgcatg gtcagcgtgt tgcattccgg aaacagcgga 35820tgatcctcca gtatcgaggc gcgggtctcc ttctcacagg gaggtaaagg gtccctgctg 35880tacggactgc gccgggacga ccgagatcgt gttgagcgta gtgtcatgga aaagggaacg 35940ccggacgtgg tcatacttct tgaagcagaa ccaggttcgc gcgtggcagg cctccttgcg 36000tctgcggtct cgccgtctag ctcgctccgt gtgatagttg tagtacagcc actcccgcag 36060agcgtcgagg cgcaccctgg cttccggatc tatgtagact ccgtcttgca ccgcggccct 36120gataatatcc accaccgtag aataagcaac acccagccaa gcaatacact cgctctgcga 36180gcggcagaca ggaggagcgg gcagagatgg gagaaccatg ataaaaaact ttttttaaag 36240aatattttcc aattcttcga aagtaagatc tatcaagtgg cagcgctccc ctccactggc 36300gcggtcaaac tctacggcca aagcacagac aacggcattt ctaagatgtt ccttaatggc 36360gtccaaaaga cacaccgctc tcaagttgca gtaaactatg aatgaaaacc catccggctg 36420attttccaat atagacgcgc cggcagcgtc caccaaaccc agataatttt cttctctcca 36480gcggtttacg atctgtctaa gcaaatccct tatatcaagt ccgaccatgc caaaaatctg 36540ctcaagagcg ccctccacct tcatgtacaa gcagcgcatc atgattgcaa aaattcaggt 36600tcttcagaga cctgtataag attcaaaatg ggaacattaa caaaaattcc tctgtcgcgc 36660agatcccttc gcagggcaag ctgaacataa tcagacaggt ccgaacggac cagtgaggcc 36720aaatccccac caggaaccag atccagagac cctatactga ttatgacgcg catactcggg 36780gctatgctga ccagcgtagc gccgatgtag gcgtgctgca tgggcggcga gataaaatgc 36840aaagtgctgg ttaaaaaatc aggcaaagcc tcgcgcaaaa aagctaacac atcataatca 36900tgctcatgca ggtagttgca ggtaagctca ggaaccaaaa cggaataaca cacgattttc 36960ctctcaaaca tgacttcgcg gatactgcgt aaaacaaaaa attataaata aaaaattaat 37020taaataactt aaacattgga agcctgtctc acaacaggaa aaaccacttt aatcaacata 37080agacgggcca cgggcatgcc ggcatagccg taaaaaaatt ggtccccgtg attaacaagt 37140accacagaca gctccccggt catgtcgggg gtcatcatgt gagactctgt atacacgtct 37200ggattgtgaa catcagacaa acaaagaaat cgagccacgt agcccggagg tataatcacc 37260cgcaggcgga ggtacagcaa aacgaccccc ataggaggaa tcacaaaatt agtaggagaa 37320aaaaatacat aaacaccaga aaaaccctgt tgctgaggca aaatagcgcc ctcccgatcc 37380aaaacaacat aaagcgcttc cacaggagca gccataacaa agacccgagt cttaccagta 37440aaagaaaaaa gatctctcaa cgcagcacca gcaccaacac ttcgcagtgt aaaaggccaa 37500gtgccgagag agtatatata ggaataaaaa gtgacgtaaa cgggcaaagt ccaaaaaacg 37560cccagaaaaa ccgcacgcga acctacgccc cgaaacgaaa gccaaaaaac actagacact 37620cccttccggc gtcaacttcc gctttcccac gctacgtcac ttcccccggt caaacaaact 37680acatatcccg aacttccaag tcgccacgcc caaaacaccg cctacacctc cccgcccgcc 37740ggcccgcccc cggacccgcc tcccgccccg cgccgcccat ctcattatca tattggcttc 37800aatccaaaat aaggtatatt attgatgatg 37830236571DNAChimpanzee adenovirus 2catcatcaat aatatacctc aaacttttgg tgcgcgttaa tatgcaaatg agctgtttga 60atttggggat gcggggcgct gattggctgc gggagcggcg accgttaggg gcggggcggg 120tgacgttttg atgacgtggc cgtgaggcgg agccggtttg caagttctcg tgggaaaagt 180gacgtcaaac gaggtgtggt ttgaacacgg aaatactcaa ttttcccgcg ctctctgaca 240ggaaatgagg tgtttctggg cggatgcaag tgaaaacggg ccattttcgc gcgaaaactg 300aatgaggaag tgaaaatctg agtaatttcg cgtttatggc agggaggagt atttgccgag 360ggccgagtag actttgaccg attacgtggg ggtttcgatt accgtatttt tcacctaaat 420ttccgcgtac ggtgtcaaag tccggtgttt ttacgtaggc gtcagctgat cgccagggta 480tttaaacctg cgctcactag tcaagaggcc actcttgagt gccagcgagt agagttttct 540cctccgcgcc gcgagtcaga tctacacttt gaaagatgag gcacttgaga gacctgcccg 600gtaatgtttt cctggctact gggaacgaga ttctggaatt ggtggtggac gccatgatgg 660gtgacgaccc tcccgagccc cctaccccat ttgaggcgcc ttcgctgtac gatttgtatg 720atctggaggt ggatgtgccc gagaacgacc ccaacgagga ggcggtgaat gatttgttta 780gcgatgccgc gctgctggct gccgagcagg ctaatacgga ctttggctca gacagcgatt 840cttctctcca taccccgaga cccggcagag gtgagaaaaa gatccccgag cttaaagggg 900aagagctcga cctgcgctgc tatgaggaat gcttgcctcc gagcgatgat gaggaggacg 960aggaggcgat tcgagctgca gcgaaccagg gagtgaaagc tgcgggcgaa agctttagcc 1020tggactgtcc tactctgccc ggacacggct gtaagtcttg tgaatttcat cgcatgaata 1080ctggagataa gaatgtgatg tgtgccctgt gctatatgag agcttacaac cattgtgttt 1140acagtaagtg tgattaactt tagttgggaa ggcagagggt gactgggtgc tgactggttt 1200atttatgtat atgtttttta tgtgtaggtc ccgtctctga cgcagatgag acccccactt 1260cagagtgcat ttcatcaccc ccagaaattg gcgaggaacc gcccgaagat attattcata 1320gaccagttgc agtgagagtc accgggcgga gagcagctgt ggagagtttg gatgacttgc 1380tacagggtgg ggatgaacct ttggacttgt gtacccggaa acgccccagg cactaagtgc 1440cacacatgtg tgtttactta aggtgatgtc agtatttata gggtgtggag tgcaataaaa 1500tccgtgttga ctttaagtgc gtggtttatg actcaggggt ggggactgtg ggtatataag 1560caggtgcaga cctgtgtggt cagttcagag caggactcat ggagatctgg acggtcttgg 1620aagactttca ccagactaga cagctgctag agaactcatc ggagggagtc tcttacctgt 1680ggagattctg cttcggtggg cctctagcta agctagtcta tagggccaag caggattata 1740aggatcaatt tgaggatatt ttgagagagt gtcctggtat ttttgactct ctcaacttgg 1800gccatcagtc tcactttaac cagagtattc tgagagccct tgacttttcc actcctggca 1860gaactaccgc cgcggtagcc ttttttgcct ttatccttga caaatggagt caagaaaccc 1920atttcagcag ggattaccgt ctggactgct tagcagtagc tttgtggaga acatggaggt 1980gccagcgcct gaatgcaatc tccggctact tgccagtaca gccggtagac acgctgagga 2040tcctgagtct ccagtcaccc caggaacacc aacgccgcca gcagccgcag caggagcagc 2100agcaagagga ggaccgagaa gagaacccga gagccggtct ggaccctccg gtggcggagg 2160aggaggagta gctgacttgt ttcccgagct gcgccgggtg ctgactaggt cttccagtgg 2220acgggagagg gggattaagc gggagaggca tgaggagact agtcacagaa ctgaactgac 2280tgtcagtctg atgagccgca ggcgcccaga atcggtgtgg tggcatgagg tgcagtcgca 2340ggggatagat gaggtctcgg tgatgcatga gaaatattcc ctagaacaag tcaagacttg 2400ttggttggag cctgaggatg attgggaggt agccatcagg aattatgcca agctagctct 2460gaagccagac aagaagtaca agattaccaa actgattaat atcagaaatt cctgctacat 2520ttcagggaat ggggccgagg tggagatcag tacccaggag agggtggcct tcagatgctg 2580catgatgaat atgtacccgg gggtggtggg catggaggga gtcaccttta tgaacgcgag 2640gttcaggggc gatgggtata atggggtggt ctttatggcc aacaccaagc tgacagtgca 2700cggatgctcc ttctttggct tcaataacat gtgcatcgag gcctggggca gtgtttcagt 2760gaggggatgc agtttttcag ccaactggat gggggtcgtg ggcagaacca agagcaaggt 2820gtcagtgaag aaatgcctgt tcgagaggtg ccacctgggg gtgatgagcg agggcgaagc 2880caaagtcaaa cactgcgcct ctactgagac gggctgcttt gtgctgatca agggcaatgc 2940ccaagtcaag cataacatga tctgtggggc ctcggatgag cgcggctacc agatgctgac 3000ctgcgccggt gggaacagcc atatgctggc caccgtgcat gtgacctcgc acccccgcaa 3060gacatggccc gagttcgagc acaacgtcat gacccgctgc aatgtgcacc tgggctcccg 3120ccgaggcatg ttcatgccct accagtgcaa catgcaattt gtgaaggtgc tgctggagcc 3180cgatgccatg tccagagtga gcctgacggg ggtgtttgac atgaatgtgg agatgtggaa 3240aattctgaga tatgatgaat ccaagaccag gtgccgggcc tgcgaatgcg gaggcaagca 3300cgccaggctt cagcccgtgt gtgtggaggt gacggaggac ctgcgacccg atcatttggt 3360gttgtcctgc aacgggacgg agttcggctc cagcggggaa gaatctgact agagtgagta 3420gtgtttgggg gaggtggagg gcctggatga ggggcagaat gactaaaatc tgtgtttttc 3480tgcgcagcag catgagcgga agcgcctcct ttgagggagg ggtattcagc ccttatctga 3540cggggcgtct cccctcctgg gcgggagtgc gtcagaatgt gatgggatcc acggtggacg 3600gccggcccgt gcagcccgcg aactcttcaa ccctgaccta cgcgaccctg agctcctcgt 3660ccgtggacgc agctgccgcc gcagctgctg cttccgccgc cagcgccgtg cgcggaatgg 3720ccctgggcgc cggctactac agctctctgg tggccaactc gagttccacc aataatcccg 3780ccagcctgaa cgaggagaag ctgctgctgc tgatggccca gctcgaggcc ctgacccagc 3840gcctgggcga gctgacccag caggttgctc agctgcaggc ggagacgcgg gccgcggttg 3900ccacggtgaa aaccaaataa aaaatgaatc aataaataaa cggagacggt tgttgatttt 3960aacacagagt cttgaatctt tatttgattt ttcgcgcgcg gtaggccctg gaccaccggt 4020ctcgatcatt gagcacccgg tggatctttt ccaggacccg gtagaggtgg gcttggatgt 4080tgaggtacat gggcatgagc ccgtcccggg ggtggaggta gctccattgc agggcctcgt 4140gctcgggggt ggtgttgtaa atcacccagt catagcaggg gcgcagggcg tggtgctgca 4200cgatgtcctt gaggaggaga ctgatggcca cgggcagccc cttggtgtag gtgttgacga 4260acctgttgag ctgggaggga tgcatgcggg gggagatgag atgcatcttg gcctggatct 4320tgagattggc gatgttcccg cccagatccc gccgggggtt catgttgtgc aggaccacca 4380gcacggtgta tccggtgcac ttggggaatt tgtcatgcaa cttggaaggg aaggcgtgaa 4440agaatttgga gacgcccttg tggccgccca ggttttccat gcactcatcc atgatgatgg 4500cgatgggccc gtgggcggcg gcctgggcaa agacgtttcg ggggtcggac acatcgtagt 4560tgtggtcctg ggtgagctcg tcataggcca ttttaatgaa tttggggcgg agggtgcccg 4620actgggggac gaaggtgccc tcgatcccgg gggcgtagtt gccctcgcag atctgcatct 4680cccaggcctt gagctcggag ggggggatca tgtccacctg cggggcgatg aaaaaaacgg 4740tttccggggc gggggagatg agctgcgccg aaagcaggtt ccggagcagc tgggacttgc 4800cgcagccggt ggggccgtag atgaccccga tgaccggctg caggtggtag ttgagggaga 4860gacagctgcc gtcctcgcgg aggagggggg ccacctcgtt catcatctcg cgcacatgca 4920tgttctcgcg cacgagttcc gccaggaggc gctcgccccc cagcgagagg agctcttgca 4980gcgaggcgaa gtttttcagc ggcttgagcc cgtcggccat gggcattttg gagagggtct 5040gttgcaagag ttccagacgg tcccagagct cggtgatgtg ctctagggca tctcgatcca 5100gcagacctcc tcgtttcgcg ggttggggcg actgcgggag tagggcacca ggcgatgggc 5160gtccagcgag gccagggtcc ggtccttcca gggtcgcagg gtccgcgtca gcgtggtctc 5220cgtcacggtg aaggggtgcg cgccgggctg ggcgcttgcg agggtgcgct tcaggctcat 5280ccggctggtc gagaaccgct cccggtcggc gccctgtgcg tcggccaggt agcaattgag 5340catgagttcg tagttgagcg cctcggccgc gtggcccttg gcgcggagct tacctttgga 5400agtgtgtccg cagacgggac agaggaggga cttgagggcg tagagcttgg gggcgaggaa 5460gacggactcg ggggcgtagg cgtccgcgcc gcagctggcg cagacggtct cgcactccac 5520gagccaggtg aggtcggggc ggtcggggtc aaaaacgagg tttcctccgt gctttttgat 5580gcgtttctta cctctggtct ccatgagctc gtgtccccgc tgggtgacaa agaggctgtc 5640cgtgtccccg tagaccgact ttatgggccg gtcctcgagc ggggtgccgc ggtcctcgtc 5700gtagaggaac cccgcccact ccgagacgaa ggcccgggtc caggccagca cgaaggaggc 5760cacgtgggag gggtagcggt cgttgtccac cagcgggtcc accttctcca gggtatgcaa 5820gcacatgtcc ccctcgtcca catccaggaa ggtgattggc ttgtaagtgt aggccacgtg 5880accgggggtc ccggccgggg gggtataaaa gggggcgggc ccctgctcgt cctcactgtc 5940ttccggatcg ctgtccagga gcgccagctg ttggggtagg tattccctct cgaaggcggg 6000catgacctcg gcactcaggt tgtcagtttc tagaaacgag gaggatttga tattgacggt 6060gccgttggag acgcctttca tgagcccctc gtccatctgg tcagaaaaga cgatcttttt 6120gttgtcgagc ttggtggcga aggagccgta gagggcgttg gagagcagct tggcgatgga 6180gcgcatggtc tggttctttt ccttgtcggc gcgctccttg gcggcgatgt tgagctgcac 6240gtactcgcgc gccacgcact tccattcggg gaagacggtg gtgagctcgt cgggcacgat 6300tctgacccgc cagccgcggt tgtgcagggt gatgaggtcc acgctggtgg ccacctcgcc 6360gcgcaggggc tcgttggtcc agcagaggcg cccgcccttg cgcgagcaga aggggggcag 6420cgggtccagc atgagctcgt cgggggggtc ggcgtccacg gtgaagatgc cgggcaggag 6480ctcggggtcg aagtagctga tgcaggtgcc cagatcgtcc agcgccgctt gccagtcgcg 6540cacggccagc gcgcgctcgt aggggctgag gggcgtgccc cagggcatgg ggtgcgtgag 6600cgcggaggcg tacatgccgc agatgtcgta gacgtagagg ggctcctcga ggacgccgat 6660gtaggtgggg tagcagcgcc ccccgcggat gctggcgcgc acgtagtcgt acagctcgtg 6720cgagggcgcg aggagccccg tgccgaggtt ggagcgttgc ggcttttcgg cgcggtagac 6780gatctggcgg aagatggcgt gggagttgga ggagatggtg ggcctctgga agatgttgaa 6840gtgggcgtgg ggcaggccga ccgagtccct gatgaagtgg gcgtaggagt cctgcagctt 6900ggcgacgagc tcggcggtga cgaggacgtc cagggcgcag tagtcgaggg tctcttggat 6960gatgtcgtac ttgagctggc ccttctgctt ccacagctcg cggttgagaa ggaactcttc 7020gcggtccttc cagtactctt cgagggggaa cccgtcctga tcggcacggt aagagcccac 7080catgtagaac tggttgacgg ccttgtaggc gcagcagccc ttctccacgg ggagggcgta 7140agcttgcgcg gccttgcgca gggaggtgtg ggtgagggcg aaggtgtcgc gcaccatgac 7200tttgaggaac tggtgcttga agtcgaggtc gtcgcagccg ccctgctccc agagttggaa

7260gtccgtgcgc ttcttgtagg cggggttggg caaagcgaaa gtaacatcgt tgaagaggat 7320cttgcccgcg cggggcatga agttgcgagt gatgcggaaa ggctggggca cctcggcccg 7380gttgttgatg acctgggcgg cgaggacgat ctcgtcgaag ccgttgatgt tgtgcccgac 7440gatgtagagt tccacgaatc gcgggcagcc cttgacgtgg ggcagcttct tgagctcgtc 7500gtaggtgagc tcggcggggt cgctgagccc gtgctgctcg agggcccagt cggcgacgtg 7560ggggttggcg ctgaggaagg aagtccagag atccacggcc agggcggtct gcaagcggtc 7620ccggtactga cggaactgct ggcccacggc cattttttcg ggggtgacgc agtagaaggt 7680gcgggggtcg ccgtgccagc ggtcccactt gagttggagg gcgaggtcgt gggcgagctc 7740gacgagcggc gggtccccgg agagtttcat gaccagcatg aaggggacga gctgcttgcc 7800gaaggacccc atccaggtgt aggtttccac atcgtaggtg aggaagagcc tttcggtgcg 7860aggatgcgag ccgatgggga agaactggat ctcctgccac cagttggagg aatggctgtt 7920gatgtgatgg aagtagaaat gccgacggcg cgccgagcac tcgtgcttgt gtttatacaa 7980gcgtccgcag tgctcgcaac gctgcacggg atgcacgtgc tgcacgagct gtacctgggt 8040tcctttgacg aggaatttca gtgggcagtg gagcgctggc ggctgcatct ggtgctgtac 8100tacgtcctgg ccatcggcgt ggccatcgtc tgcctcgatg gtggtcatgc tgacgagccc 8160gcgcgggagg caggtccaga cctcggctcg gacgggtcgg agagcgagga cgagggcgcg 8220caggccggag ctgtccaggg tcctgagacg ctgcggagtc aggtcagtgg gcagcggcgg 8280cgcgcggttg acttgcagga gcttttccag ggcgcgcggg aggtccagat ggtacttgat 8340ctccacggcg ccgttggtgg cgacgtccac ggcttgcagg gtcccgtgcc cctggggcgc 8400caccaccgtg ccccgtttct tcttgggcgg cggcggctcc atgcttagaa gcggcggcga 8460ggacgcgcgc cgggcggcag gggcggctcg gggcccggag gcaggggcgg caggggcacg 8520tcggcgccgc gcgcgggcag gttctggtac tgcgcccgga gaagactggc gtgagcgacg 8580acgcgacggt tgacgtcctg gatctgacgc ctctgggtga aggccacggg acccgtgagt 8640ttgaacctga aagagagttc gacagaatca atttcggtat cgttgacggc ggcctgccgc 8700aggatctctt gcacgtcgcc cgagttgtcc tggtaggcga tctcggtcat gaactgctcg 8760atctcctcct cctgaaggtc tccgcggccg gcgcgctcga cggtggccgc gaggtcgttg 8820gagatgcggc ccatgagctg cgagaaggcg ttcatgccgg cctcgttcca gacgcggctg 8880tagaccacgg ctccgttggg gtcgcgcgcg cgcatgacca cctgggcgag gttaagctcg 8940acgtggcgcg tgaagaccgc gtagttgcag aggcgctggt agaggtagtt gagcgtggtg 9000gcgatgtgct cggtgacgaa gaagtacatg atccagcggc ggagcggcat ctcgctgacg 9060tcgcccaggg cttccaagcg ctccatggtc tcgtagaagt ccacggcgaa gttgaaaaac 9120tgggagttgc gcgccgagac ggtcaactcc tcctccagaa gacggatgag ctcggcgatg 9180gtggcgcgca cctcgcgctc gaaggccccg gggggctcct cttcttccat ctcctcctcc 9240tcttcctcct ccactaacat ctcttctact tcctcctcag gaggcggcgg cgggggaggg 9300gccctgcgtc gccggcggcg cacgggcaga cggtcgatga agcgctcgat ggtctccccg 9360cgccggcgac gcatggtctc ggtgacggcg cgcccgtcct cgcggggccg cagcgtgaag 9420acgccgccgc gcatctccag gtggccgccg ggggggtctc cgttgggcag ggagagggcg 9480ctgacgatgc atcttatcaa ttggcccgta gggactccgc gcaaggacct gagcgtctcg 9540agatccacgg gatccgaaaa ccgctgaacg aaggcttcga gccagtcgca gtcgcaaggt 9600aggctgagcc cggtttcttg ttcttcgggt atttggtcgg gaggcgggcg ggcgatgctg 9660ctggtgatga agttgaagta ggcggtcctg agacggcgga tggtggcgag gagcaccagg 9720tccttgggcc cggcttgctg gatgcgcaga cggtcggcca tgccccaggc gtggtcctga 9780cacctggcga ggtccttgta gtagtcctgc atgagccgct ctacgggcac gtcctcctcg 9840cccgcgcggc cgtgcatgcg cgtgagcccg aacccgcgct gcggctggac gagcgccagg 9900tcggcgacga cgcgctcggc gaggatggcc tgctggatct gggtgagggt ggtctggaag 9960tcgtcgaagt cgacgaagcg gtggtaggct ccggtgttga tggtgtagga gcagttggcc 10020atgacggacc agttgacggt ctggtggccg gggcgcacga gctcgtggta cttgaggcgc 10080gagtaggcgc gcgtgtcgaa gatgtagtcg ttgcaggtgc gcacgaggta ctggtatccg 10140acgaggaagt gcggcggcgg ctggcggtag agcggccatc gctcggtggc gggggcgccg 10200ggcgcgaggt cctcgagcat gaggcggtgg tagccgtaga tgtacctgga catccaggtg 10260atgccggcgg cggtggtgga ggcgcgcggg aactcgcgga cgcggttcca gatgttgcgc 10320agcggcagga agtagttcat ggtggccgcg gtctggcccg tgaggcgcgc gcagtcgtgg 10380atgctctaga catacgggca aaaacgaaag cggtcagcgg ctcgactccg tggcctggag 10440gctaagcgaa cgggttgggc tgcgcgtgta ccccggttcg aatctcgaat caggctggag 10500ccgcagctaa cgtggtactg gcactcccgt ctcgacccaa gcctgctaac gaaacctcca 10560ggatacggag gcgggtcgtt ttttggcctt ggtcgctggt catgaaaaac tagtaagcgc 10620ggaaagcggc cgcccgcgat ggctcgctgc cgtagtctgg agaaagaatc gccagggttg 10680cgttgcggtg tgccccggtt cgagcctcag cgctcggtgc cggccggatt ccgcggctaa 10740cgtgggcgtg gctgccccgt cgtttccaag accccttagc cagccgactt ctccagttac 10800ggagcgagcc cctctttttc ttgtgttttt gccagatgca tcccgtactg cggcagatgc 10860gcccccaccc tccaccacaa ccgcccctac cgcagcagca gcaacagccg gcgcttctgc 10920ccccgcccca gcagcagcag ccagccacta ccgcggcggc cgccgtgagc ggagccggcg 10980ttcagtatga cctggccttg gaagagggcg aggggctggc gcggctgggg gcgtcgtcgc 11040cggagcggca cccgcgcgtg cagatgaaaa gggacgctcg cgaggcctac gtgcccaagc 11100agaacctgtt cagagacagg agcggcgagg agcccgagga gatgcgcgcc tcccgcttcc 11160acgcggggcg ggagctgcgg cgcggcctgg accgaaagcg ggtgctgagg gacgaggatt 11220tcgaggcgga cgagctgacg gggatcagcc ccgcgcgcgc gcacgtggcc gcggccaacc 11280tggtcacggc gtacgagcag accgtgaagg aggagagcaa ctttcaaaaa tccttcaaca 11340accacgtgcg cacgctgatc gcgcgcgagg aggtgaccct gggcctgatg cacctgtggg 11400acctgctgga ggccatcgtg cagaacccca cgagcaagcc gctgacggcg cagctgtttc 11460tggtggtgca gcacagtcgg gacaacgaga cgttcaggga ggcgctgctg aatatcaccg 11520agcccgaggg ccgctggctc ctggacctgg tgaacattct gcagagcatc gtggtgcagg 11580agcgcgggct gccgctgtcc gagaagctgg cggccatcaa cttctcggtg ctgagcctgg 11640gcaagtacta cgctaggaag atctacaaga ccccgtacgt gcccatagac aaggaggtga 11700agatcgacgg gttttacatg cgcatgaccc tgaaagtgct gaccctgagc gacgatctgg 11760gggtgtaccg caacgacagg atgcaccgcg cggtgagcgc cagccgccgg cgcgagctga 11820gcgaccagga gctgatgcac agcctgcagc gggccctgac cggggccggg accgaggggg 11880agagctactt tgacatgggc gcggacctgc gctggcagcc cagccgccgg gccttggaag 11940ctgccggcgg cgtgccctac gtggaggagg tggacgatga ggaggaggag ggcgagtacc 12000tggaagactg atggcgcgac cgtatttttg ctagatgcag caacagccac cgccgcctcc 12060tgatcccgcg atgcgggcgg cgctgcagag ccagccgtcc ggcattaact cctcggacga 12120ttggacccag gccatgcaac gcatcatggc gctgacgacc cgcaatcccg aagcctttag 12180acagcagcct caggccaacc ggctctcggc catcctggag gccgtggtgc cctcgcgctc 12240gaaccccacg cacgagaagg tgctggccat cgtgaacgcg ctggtggaga acaaggccat 12300ccgcggcgac gaggccgggc tggtgtacaa cgcgctgctg gagcgcgtgg cccgctacaa 12360cagcaccaac gtgcagacga acctggaccg catggtgacc gacgtgcgcg aggcggtgtc 12420gcagcgcgag cggttccacc gcgagtcgaa cctgggctcc atggtggcgc tgaacgcctt 12480cctgagcacg cagcccgcca acgtgccccg gggccaggag gactacacca acttcatcag 12540cgcgctgcgg ctgatggtgg ccgaggtgcc ccagagcgag gtgtaccagt cggggccgga 12600ctacttcttc cagaccagtc gccagggctt gcagaccgtg aacctgagcc aggctttcaa 12660gaacttgcag ggactgtggg gcgtgcaggc cccggtcggg gaccgcgcga cggtgtcgag 12720cctgctgacg ccgaactcgc gcctgctgct gctgctggtg gcgcccttca cggacagcgg 12780cagcgtgagc cgcgactcgt acctgggcta cctgcttaac ctgtaccgcg aggccatcgg 12840gcaggcgcac gtggacgagc agacctacca ggagatcacc cacgtgagcc gcgcgctggg 12900ccaggaggac ccgggcaacc tggaggccac cctgaacttc ctgctgacca accggtcgca 12960gaagatcccg ccccagtacg cgctgagcac cgaggaggag cgcatcctgc gctacgtgca 13020gcagagcgtg gggctgttcc tgatgcagga gggggccacg cccagcgccg cgctcgacat 13080gaccgcgcgc aacatggagc ccagcatgta cgcccgcaac cgcccgttca tcaataagct 13140gatggactac ttgcatcggg cggccgccat gaactcggac tactttacca acgccatctt 13200gaacccgcac tggctcccgc cgcccgggtt ctacacgggc gagtacgaca tgcccgaccc 13260caacgacggg ttcctgtggg atgacgtgga cagcagcgtg ttctcgccgc gtcccaccac 13320caccgtgtgg aagaaagagg gcggggaccg gcggccgtcc tcggcgctgt ccggtcgcgc 13380gggtgctgcc gcggcggtgc ccgaggccgc cagccccttt ccgagcctgc ccttttcgct 13440gaacagcgtg cgcagcagcg agctgggtcg gctgacgcgg ccgcgcctgc tgggcgagga 13500ggagtacctg aacgactcct tgttgaggcc cgagcgcgaa aagaacttcc ccaataacgg 13560gatagagagc ctggtggaca agatgagccg ctggaagacg tacgcgcacg agcacaggga 13620cgagccccga gctagcagcg caggcacccg tagacgccag cggcacgaca ggcagcgggg 13680tctggtgtgg gacgatgagg attccgccga cgacagcagc gtgttggact tgggtgggag 13740tggtggtggt aacccgttcg ctcacttgcg cccccgtatc gggcgcctga tgtaagaatc 13800tgaaaaataa aaaacggtac tcaccaaggc catggcgacc agcgtgcgtt cttctctgtt 13860gtttgtagta gtatgatgag gcgcgtgtac ccggagggtc ctcctccctc gtacgagagc 13920gtgatgcagc aggcggtggc ggcggcgatg cagcccccgc tggaggcgcc ttacgtgccc 13980ccgcggtacc tggcgcctac ggaggggcgg aacagcattc gttactcgga gctggcaccc 14040ttgtacgata ccacccggtt gtacctggtg gacaacaagt cggcggacat cgcctcgctg 14100aactaccaga acgaccacag caacttcctg accaccgtgg tgcagaacaa cgatttcacc 14160cccacggagg ccagcaccca gaccatcaac tttgacgagc gctcgcggtg gggcggccag 14220ctgaaaacca tcatgcacac caacatgccc aacgtgaacg agttcatgta cagcaacaag 14280ttcaaggcgc gggtgatggt ctcgcgcaag acccccaacg gggtcacagt aacagatggt 14340agtcaggacg agctgaccta cgagtgggtg gagtttgagc tgcccgaggg caacttctcg 14400gtgaccatga ccatcgatct gatgaacaac gccatcatcg acaactactt ggcggtgggg 14460cggcagaacg gggtgctgga gagcgacatc ggcgtgaagt tcgacacgcg caacttccgg 14520ctgggctggg accccgtgac cgagctggtg atgccgggcg tgtacaccaa cgaggccttc 14580caccccgaca tcgtcctgct gcccggctgc ggcgtggact tcaccgagag ccgcctcagc 14640aacctgctgg gcatccgcaa gcggcagccc ttccaggagg gcttccagat cctgtacgag 14700gacctggagg ggggcaacat ccccgcgctc ttggatgtcg aagcctacga gaaaagcaag 14760gaggatagca ccgccgtggc taccgccgcg actgtggcag atgccactgt caccaggggc 14820gatacattcg ccacccaggc ggaggaagca gccgccctag cggcgaccga tgatagtgaa 14880agtaagatag ttatcaagcc ggtggagaag gacagcaagg acaggagcta caacgttcta 14940tcggatggaa agaacaccgc ctaccgcagc tggtacctgg cctacaacta cggcgacccc 15000gagaagggcg tgcgctcctg gacgctgctc accacctcgg acgtcacctg cggcgtggag 15060caagtctact ggtcgctgcc cgacatgatg caagacccgg tcaccttccg ctccacgcgt 15120caagttagca actacccggt ggtgggcgcc gagctcctgc ccgtctactc caagagcttc 15180ttcaacgagc aggccgtcta ctcgcagcag ctgcgcgcct tcacctcgct cacgcacgtc 15240ttcaaccgct tccccgagaa ccagatcctc gtccgcccgc ccgcgcccac cattaccacc 15300gtcagtgaaa acgttcctgc tctcacagat cacgggaccc tgccgctgcg cagcagtatc 15360cggggagtcc agcgcgtgac cgtcactgac gccagacgcc gcacctgccc ctacgtctac 15420aaggccctgg gcgtagtcgc gccgcgcgtc ctctcgagcc gcaccttcta aaaaatgtcc 15480attctcatct cgcccagtaa taacaccggt tggggcctgc gcgcgcccag caagatgtac 15540ggaggcgctc gccaacgctc cacgcaacac cccgtgcgcg tgcgcgggca cttccgcgct 15600ccctggggcg ccctcaaggg tcgcgtgcgc tcgcgcacca ccgtcgacga cgtgatcgac 15660caggtggtgg ccgacgcgcg caactacacg cccgccgccg cgcccgcctc caccgtggac 15720gccgtcatcg acagcgtggt ggccgacgcg cgccggtacg cccgcgccaa gagccggcgg 15780cggcgcatcg cccggcggca ccggagcacc cccgccatgc gcgcggcgcg agccttgctg 15840cgcagggcca ggcgcacggg acgcagggcc atgctcaggg cggccagacg cgcggcctcc 15900ggcagcagca gcgccggcag gacccgcaga cgcgcggcca cggcggcggc ggcggccatc 15960gccagcatgt cccgcccgcg gcgcggcaac gtgtactggg tgcgcgacgc cgccaccggt 16020gtgcgcgtgc ccgtgcgcac ccgcccccct cgcacttgaa gatgctgact tcgcgatgtt 16080gatgtgtccc agcggcgagg aggatgtcca agcgcaaata caaggaagag atgctccagg 16140tcatcgcgcc tgagatctac ggccccgcgg cggcggtgaa ggaggaaaga aagccccgca 16200aactgaagcg ggtcaaaaag gacaaaaagg aggaggaaga tgtggacgga ctggtggagt 16260ttgtgcgcga gttcgccccc cggcggcgcg tgcagtggcg cgggcggaaa gtgaaaccgg 16320tgctgcggcc cggcaccacg gtggtcttca cgcccggcga gcgttccggc tccgcctcca 16380agcgctccta cgacgaggtg tacggggacg aggacatcct cgagcaggcg gccgagcgtc 16440tgggcgagtt tgcttacggc aagcgcagcc gccccgcgcc cttgaaagag gaggcggtgt 16500ccatcccgct ggaccacggc aaccccacgc cgagcctgaa gccggtgacc ctgcagcagg 16560tgctgccgag cgcggcgccg cgccggggct tcaagcgcga gggcggcgag gatctgtacc 16620cgaccatgca gctgatggtg cccaagcgcc agaagctgga ggacgtgctg gagcacatga 16680aggtggaccc cgaggtgcag cccgaggtca aggtgcggcc catcaagcag gtggccccgg 16740gcctgggcgt gcagaccgtg gacatcaaga tccccacgga gcccatggaa acgcagaccg 16800agcccgtgaa gcccagcacc agcaccatgg aggtgcagac ggatccctgg atgccggcgc 16860cggcttccac caccactcgc cgaagacgca agtacggcgc ggccagcctg ctgatgccca 16920actacgcgct gcatccttcc atcatcccca cgccgggcta ccgcggcacg cgcttctacc 16980gcggctacag cagccgccgc aagaccacca cccgccgccg ccgtcgccgc acccgccgca 17040gcaccaccgc gacttccgcc gccgccttgg tgcggagagt gtaccgcagc gggcgtgagc 17100ctctgaccct gccgcgcgcg cgctaccacc cgagcatcgc catttaactc tgccgtcgcc 17160tccttgcaga tatggccctc acatgccgcc tccgcgtccc cattacgggc taccgaggaa 17220gaaagccgcg ccgtagaagg ctgacgggga acgggctgcg tcgccatcac caccggcggc 17280ggcgcgccat cagcaagcgg ttggggggag gcttcctgcc cgcgctgatc cccatcatcg 17340ccgcggcgat cggggcgatc cccggcatag cttccgtggc ggtgcaggcc tctcagcgcc 17400actgagacac agcttggaaa atttgtaata aaaaaatgga ctgacgctcc tggtcctgtg 17460atgtgtgttt ttagatggaa gacatcaatt tttcgtccct ggcaccgcga cacggcacgc 17520ggccgtttat gggcacctgg agcgacatcg gcaacagcca actgaacggg ggcgccttca 17580attggagcag tctctggagc gggcttaaga atttcgggtc cacgctcaaa acctatggca 17640acaaggcgtg gaacagcagc acagggcagg cgctgaggga aaagctgaaa gagcagaact 17700tccagcagaa ggtggtcgat ggcctggcct cgggcatcaa cggggtggtg gacctggcca 17760accaggccgt gcagaaacag atcaacagcc gcctggacgc ggtcccgccc gcggggtccg 17820tggagatgcc ccaggtggag gaggagctgc ctcccctgga caagcgcggc gacaagcgac 17880cgcgtcccga cgcggaggag acgctgctga cgcacacgga cgagccgccc ccgtacgagg 17940aggcggtgaa actgggtctg cccaccacgc ggcccgtggc gcctctggcc accggggtgc 18000tgaaacccag cagcagcagc agccagcccg cgaccctgga cttgcctcca cctcgcccct 18060ccacagtggc taagcccctg ccgccggtgg ccgtcgcgtc gcgcgccccc cgaggccgcc 18120cccaggcgaa ctggcagagc actctgaaca gcatcgtggg tctgggagtg cagagtgtga 18180agcgccgccg ctgctattaa aagacactgt agcgcttaac ttgcttgtct gtgtgtatat 18240gtatgtccgc cgaccagaag gaggaggaag aggcgcgtcg ccgagttgca agatggccac 18300cccatcgatg ctgccccagt gggcgtacat gcacatcgcc ggacaggacg cttcggagta 18360cctgagtccg ggtctggtgc agttcgcccg cgccacagac acctacttca gtctggggaa 18420caagtttagg aaccccacgg tggcacccac gcacgatgtg accaccgacc gcagccagcg 18480gctgacgctg cgcttcgtgc ccgtggaccg cgaggacaac acctactcgt acaaagtgcg 18540ctacacgctg gccgtgggcg acaaccgcgt gctggacatg gccagcacct actttgacat 18600ccgcggcgtg ctggatcggg gccccagctt caaaccctac tccggcaccg cctacaacag 18660cctggctccc aagggagcgc ccaacacctc acagtggata accaaagaca atggaactga 18720taagacatac agttttggaa atgctccagt cagaggattg gacattacag aagagggtct 18780ccaaatagga accgatgagt cagggggtga aagcaagaaa atttttgcag acaaaaccta 18840tcagcctgaa cctcagcttg gagatgagga atggcatgat actattggag ctgaagacaa 18900gtatggaggc agagcgctta aacctgccac caacatgaaa ccctgctatg ggtctttcgc 18960caagccaact aatgctaagg gaggtcaggc taaaagcaga accaaggacg atggcactac 19020tgagcctgat attgacatgg ccttctttga cgatcgcagt cagcaagcta gtttcagtcc 19080agaacttgtt ttgtatactg agaatgtcga tctggacacc ccggataccc acattattta 19140caaacctggc actgatgaaa caagttcttc tttcaacttg ggtcagcagt ccatgcccaa 19200cagacccaac tacattggct tcagagacaa ctttatcggg ctcatgtact acaacagcac 19260tggcaatatg ggtgtactgg ccggtcaggc ctcccagctg aatgctgtgg tggacttgca 19320ggacagaaac actgaactgt cctaccagct cttgcttgac tctctgggtg acagaaccag 19380gtatttcagt atgtggaatc aggcggtgga cagctatgac cccgatgtgc gcattattga 19440aaatcacggt gtggaggatg aactccccaa ctattgcttc cctttgaatg gtgtgggctt 19500tacagataca ttccagggaa ttaaggttaa aactacaaat aacggaacag caaatgctac 19560agagtgggaa tctgatacct ctgtcaataa tgctaatgag attgccaagg gcaatccttt 19620cgccatggag atcaacatcc aggccaacct gtggcggaac ttcctctacg cgaacgtggc 19680gctgtacctg cccgactcct acaagtacac gccggccaac atcacgctgc ccaccaacac 19740caacacctac gattacatga acggccgcgt ggtggcgccc tcgctggtgg acgcctacat 19800caacatcggg gcgcgctggt cgctggaccc catggacaac gtcaacccct tcaaccacca 19860ccgcaacgcg ggcctgcgct accgctccat gctcctgggc aacgggcgct acgtgccctt 19920ccacatccag gtgccccaaa agtttttcgc catcaagagc ctcctgctcc tgcccgggtc 19980ctacacctac gagtggaact tccgcaagga cgtcaacatg atcctgcaga gctccctcgg 20040caacgacctg cgcacggacg gggcctccat cgccttcacc agcatcaacc tctacgccac 20100cttcttcccc atggcgcaca acaccgcctc cacgctcgag gccatgctgc gcaacgacac 20160caacgaccag tccttcaacg actacctctc ggcggccaac atgctctacc ccatcccggc 20220caacgccacc aacgtgccca tctccatccc ctcgcgcaac tgggccgcct tccgcggatg 20280gtccttcacg cgcctcaaga cccgcgagac gccctcgctc ggctccgggt tcgaccccta 20340cttcgtctac tcgggctcca tcccctacct cgacggcacc ttctacctca accacacctt 20400caagaaggtc tccatcacct tcgactcctc cgtcagctgg cccggcaacg accgcctcct 20460gacgcccaac gagttcgaaa tcaagcgcac cgtcgacgga gaggggtaca acgtggccca 20520gtgcaacatg accaaggact ggttcctggt ccagatgctg gcccactaca acatcggcta 20580ccagggcttc tacgtgcccg agggctacaa ggaccgcatg tactccttct tccgcaactt 20640ccagcccatg agccgccagg tcgtggacga ggtcaactac aaggactacc aggccgtcac 20700cctggcctac cagcacaaca actcgggctt cgtcggctac ctcgcgccca ccatgcgcca 20760gggccagccc taccccgcca actaccccta cccgctcatc ggcaagagcg ccgtcgccag 20820cgtcacccag aaaaagttcc tctgcgaccg ggtcatgtgg cgcatcccct tctccagcaa 20880cttcatgtcc atgggcgcgc tcaccgacct cggccagaac atgctctacg ccaactccgc 20940ccacgcgcta gacatgaatt tcgaagtcga ccccatggat gagtccaccc ttctctatgt 21000tgtcttcgaa gtcttcgacg tcgtccgagt gcaccagccc caccgcggcg tcatcgaggc 21060cgtctacctg cgcacgccct tctcggccgg caacgccacc acctaagcct cttgcttctt 21120gcaagatgac ggcctgtggc tccggcgagc aggagctcag ggccatcctc cgcgacctgg 21180gctgcgggcc ctacttcctg ggcaccttcg acaagcgctt cccgggattc atggccccgc 21240acaagctggc ctgcgccatc gtcaacacgg ccggccgcga gaccgggggc gagcactggc 21300tggccttcgc ctggaacccg cgcacccaca cctgctacct cttcgacccc ttcgggttct 21360cggacgagcg cctcaagcag atctaccagt tcgagtacga gggcctgctg cgccgcagcg 21420ccctggccac cgaggaccgc tgcgtcaccc tggaaaagtc cacccagacc gtgcagggtc 21480cgcgctcggc cgcctgcggg ctcttctgct gcatgttcct gcacgccttc gtgcactggc 21540ccgaccgccc catggacaag aaccccacca tgaacttgct gacgggggtg cccaacggca 21600tgctccagtc gccccaggtg gaacccaccc tgcgccgcaa ccaggaggcg ctctaccgct 21660tcctcaacgc ccactccgcc tactttcgct cccaccgcgc gcgcatcgag aaggccaccg 21720ccttcgaccg catgaatcaa gacatgtaaa ctgtgtgtat gtgaatgctt tattcataat 21780aaacagcaca tgtttatgcc accttctctg aggctctgac tttatttaga aatcgaaggg 21840gttctgccgg ctctcggcgt gccccgcggg cagggatacg ttgcggaact ggtacttggg 21900cagccacttg aactcgggga tcagcagctt cggcacgggg aggtcgggga acgagtcgct 21960ccacagcttg cgcgtgagtt gcagggcgcc cagcaggtcg ggcgcggata tcttgaaatc 22020acagttggga cccgcgttct gcgcgcgaga gttgcggtac acggggttgc agcactggaa 22080caccatcagg gccgggtgct tcacgctcgc cagcaccgtc gcgtcggtga tgccctccac 22140gtccagatcc tcggcgttgg ccatcccgaa gggggtcatc ttgcaggtct gccgccccat 22200gctgggcacg cagccgggct tgtggttgca atcgcagtgc agggggatca gcatcatctg 22260ggcctgctcg gagctcatgc ccgggtacat ggccttcatg aaagcctcca gctggcggaa

22320ggcctgctgc gccttgccgc cctcggtgaa gaagaccccg caggacttgc tagagaactg 22380gttggtggcg cagccggcgt cgtgcacgca gcagcgcgcg tcgttgttgg ccagctgcac 22440cacgctgcgc ccccagcggt tctgggtgat cttggcccgg tcggggttct ccttcagcgc 22500gcgctgcccg ttctcgctcg ccacatccat ctcgatcgtg tgctccttct ggatcatcac 22560ggtcccgtgc aggcaccgca gcttgccctc ggcttcggtg catccgtgca gccacagcgc 22620gcagccggtg cactcccagt tcttgtgggc gatctgggag tgcgagtgca cgaagccctg 22680caggaagcgg cccatcatcg cggtcagggt cttgttgctg gtgaaggtca gcgggatgcc 22740gcggtgctcc tcgttcacat acaggtggca gatgcggcgg tacacctcgc cctgctcggg 22800catcagctgg aaggcggact tcaggtcgct ctccacgcgg taccgctcca tcagcagcgt 22860catgacttcc atgcccttct cccaggccga aacgatcggc aggctcaggg ggttcttcac 22920cgttgtcatc ttagtcgccg ccgccgaggt cagggggtcg ttctcgtcca gggtctcaaa 22980cactcgcttg ccgtccttct cggtgatgcg cacgggggga aagctgaagc ccacggccgc 23040cagctcctcc tcggcctgcc tttcgtcctc gctgtcctgg ctgatgtctt gcaaaggcac 23100atgcttggtc ttgcggggtt tctttttggg cggcagaggc ggcggcggag acgtgctggg 23160cgagcgcgag ttctcgctca ccacgactat ttcttcttct tggccgtcgt ccgagaccac 23220gcggcggtag gcatgcctct tctggggcag aggcggaggc gacgggctct cgcggttcgg 23280cgggcggctg gcagagcccc ttccgcgttc gggggtgcgc tcctggcggc gctgctctga 23340ctgacttcct ccgcggccgg ccattgtgtt ctcctaggga gcaagcatgg agactcagcc 23400atcgtcgcca acatcgccat ctgcccccgc cgccgccgac gagaaccagc agcagcagaa 23460tgaaagctta accgccccgc cgcccagccc cacctccgac gccgcggccc cagacatgca 23520agagatggag gaatccatcg agattgacct gggctacgtg acgcccgcgg agcacgagga 23580ggagctggca gcgcgctttt cagccccgga agagaaccac caagagcagc cagagcagga 23640agcagagagc gagcagagcc aggctgggct cgagcatggc gactacctga gcggggcaga 23700ggacgtgctc atcaagcatc tggcccgcca atgcatcatc gtcaaggatg cgctgctcga 23760ccgcgccgag gtgcccctca gcgtggcgga gctcagccgc gcctacgagc gcaacctctt 23820ctcgccgcgc gtgcccccca agcgccagcc caacggcacc tgcgagccca acccgcgcct 23880caacttctac ccggtcttcg cggtgcccga ggccctggcc acctaccacc tctttttcaa 23940gaaccaaagg atccccgtct cctgccgcgc caaccgcacc cgcgccgacg ccctgctcaa 24000cctgggcccc ggcgcccgcc tacctgatat cgcctccttg gaagaggttc ccaagatctt 24060cgagggtctg ggcagcgacg agactcgggc cgcgaacgct ctgcaaggaa gcggagagga 24120gcatgagcac cacagcgccc tggtggagtt ggaaggcgac aacgcgcgcc tggcggtcct 24180caagcgcacg gtcgagctga cccacttcgc ctacccggcg ctcaacctgc cccccaaggt 24240catgagcgcc gtcatggacc aggtgctcat caagcgcgcc tcgcccctct cggaggagga 24300gatgcaggac cccgagagct cggacgaggg caagcccgtg gtcagcgacg agcagctggc 24360gcgctggctg ggagcgagta gcacccccca gagcctggaa gagcggcgca agctcatgat 24420ggccgtggtc ctggtgaccg tggagctgga gtgtctgcgc cgcttcttcg ccgacgcgga 24480gaccctgcgc aaggtcgagg agaacctgca ctacctcttc aggcacgggt tcgtgcgcca 24540ggcctgcaag atctccaacg tggagctgac caacctggtc tcctacatgg gcatcctgca 24600cgagaaccgc ctggggcaga acgtgctgca caccaccctg cgcggggagg cccgccgcga 24660ctacatccgc gactgcgtct acctgtacct ctgccacacc tggcagacgg gcatgggcgt 24720gtggcagcag tgcctggagg agcagaacct gaaagagctc tgcaagctcc tgcagaagaa 24780cctgaaggcc ctgtggaccg ggttcgacga gcgcaccacc gcctcggacc tggccgacct 24840catcttcccc gagcgcctgc ggctgacgct gcgcaacggg ctgcccgact ttatgagcca 24900aagcatgttg caaaactttc gctctttcat cctcgaacgc tccgggatcc tgcccgccac 24960ctgctccgcg ctgccctcgg acttcgtgcc gctgaccttc cgcgagtgcc ccccgccgct 25020ctggagccac tgctacctgc tgcgtctggc caactacctg gcctaccact cggacgtgat 25080cgaggacgtc agcggcgagg gtctgctcga gtgccactgc cgctgcaacc tctgcacgcc 25140gcaccgctcc ctggcctgca acccccagct gctgagcgag acccagatca tcggcacctt 25200cgagttgcaa ggccccggcg aggagggcaa ggggggtctg aaactcaccc cggggctgtg 25260gacctcggcc tacttgcgca agttcgtgcc cgaggactac catcccttcg agatcaggtt 25320ctacgaggac caatcccagc cgcccaaggc cgagctgtcg gcctgcgtca tcacccaggg 25380ggccatcctg gcccaattgc aagccatcca gaaatcccgc caagaatttc tgctgaaaaa 25440gggccacggg gtctacttgg acccccagac cggagaggag ctcaacccca gcttccccca 25500ggatgcccag aggaagcagc aagaagctga aagtggagct gccgctgccg ccggaggatt 25560tggaggaaga ctgggagagc agtcaggcag aggaggagga gatggaagac tgggacagca 25620ctcaggcaga ggaggacagc ctgcaagaca gtctggaaga cgaggtggag gaggaggcag 25680aggaagaagc agccgccgcc agaccgtcgt cctcggcgga gaaagcaagc agcacggata 25740ccatctccgc tccgggtcgg ggtctcggcg gccgggccca cagtaggtgg gacgagaccg 25800ggcgcttccc gaaccccacc acccagaccg gtaagaagga gcggcaggga tacaagtcct 25860ggcgggggca caaaaacgcc atcgtctcct gcttgcaagc ctgcgggggc aacatctcct 25920tcacccggcg ctacctgctc ttccaccgcg gggtgaactt cccccgcaac atcttgcatt 25980actaccgtca cctccacagc ccctactact gtttccaaga agaggcagaa acccagcagc 26040agcagaaaac cagcagcagc tagaaaatcc acagcggcgg cggcggcagg tggactgagg 26100atcgcggcga acgagccggc gcagacccgg gagctgagga accggatctt tcccaccctc 26160tatgccatct tccagcagag tcgggggcag gagcaggaac tgaaagtcaa gaaccgttct 26220ctgcgctcgc tcacccgcag ttgtctgtat cacaagagcg aagaccaact tcagcgcact 26280ctcgaggacg ccgaggctct cttcaacaag tactgcgcgc tcactcttaa agagtagccc 26340gcgcccgccc acacacggaa aaaggcggga attacgtcac cacctgcgcc cttcgcccga 26400ccatcatcat gagcaaagag attcccacgc cttacatgtg gagctaccag ccccagatgg 26460gcctggccgc cggcgccgcc caggactact ccacccgcat gaactggctc agtgccgggc 26520ccgcgatgat ctcacgggtg aatgacatcc gcgcccgccg aaaccagata ctcctagaac 26580agtcagcgat caccgccacg ccccgccatc accttaatcc gcgtaattgg cccgccgccc 26640tggtgtacca ggaaattccc cagcccacga ccgtactact tccgcgagac gcccaggccg 26700aagtccagct gactaactca ggtgtccagc tggccggcgg cgccgccctg tgtcgtcacc 26760gccccgctca gggtataaag cggctggtga tccgaggcag aggcacacag ctcaacgacg 26820aggtggtgag ctcttcgctg ggtctgcgac ctgacggagt cttccaactc gccggatcgg 26880ggagatcttc cttcacgcct cgtcaggccg tcctgacttt ggagagttcg tcctcgcagc 26940cccgctcggg tggcatcggc actctccagt tcgtggagga gttcactccc tcggtctact 27000tcaacccctt ctccggctcc cccggccact acccggacga gttcatcccg aacttcgacg 27060ccatcagcga gtcggtggac ggctacgatt gaatgtccca tggtggcgcg gctgacctag 27120ctcggcttcg acacctggac cactgccgcc gcttccgctg cttcgctcgg gatctcgccg 27180agtttgccta ctttgagctg cccgaggagc accctcaggg cccggcccac ggagtgcgga 27240tcatcgtcga agggggcctc gactcccacc tgcttcggat cttcagccag cgtccgatcc 27300tggtcgagcg cgagcaagga cagacccgtc tgaccctgta ctgcatctgc aaccaccccg 27360gcctgcatga aagtctttgt tgtctgctgt gtactgagta taataaaagc tgagatcagc 27420gactactccg gacttccgtg tgttcctgaa tccatcaacc agtccctgtt cttcaccggg 27480aacgagaccg agctccagct ccagtgtaag ccccacaaga agtacctcac ctggctgttc 27540cagggctccc cgatcgccgt tgtcaaccac tgcgacaacg acggagtcct gctgagcggc 27600cctgccaacc ttactttttc cacccgcaga agcaagctcc agctcttcca acccttcctc 27660cccgggacct atcagtgcgt ctcgggaccc tgccatcaca ccttccacct gatcccgaat 27720accacagcgt cgctccccgc tactaacaac caaactaccc accaacgcca ccgtcgcgac 27780ctttcctctg aatctaatac cactaccgga ggtgagctcc gaggtcgacc aacctctggg 27840atttactacg gcccctggga ggtggtgggg ttaatagcgc taggcctagt tgtgggtggg 27900cttttggctc tctgctacct atacctccct tgctgttcgt acttagtggt gctgtgttgc 27960tggtttaaga aatggggcag atcaccctag tgagctgcgg tgtgctggtg gcggtggtgc 28020tttcgattgt gggactgggc ggcgcggctg tagtgaagga gaaggccgat ccctgcttgc 28080atttcaatcc cgacaaatgc cagctgagtt ttcagcccga tggcaatcgg tgcgcggtgc 28140tgatcaagtg cggatgggaa tgcgagaacg tgagaatcga gtacaataac aagactcgga 28200acaatactct cgcgtccgtg tggcagcccg gggaccccga gtggtacacc gtctctgtcc 28260ccggtgctga cggctccccg cgcaccgtga ataatacttt catttttgcg cacatgtgcg 28320acacggtcat gtggatgagc aagcagtacg atatgtggcc ccccacgaag gagaacatcg 28380tggtcttctc catcgcttac agcctgtgca cggtgctaat caccgctatc gtgtgcctga 28440gcattcacat gctcatcgct attcgcccca gaaataatgc cgaaaaagag aaacagccat 28500aacacgtttt ttcacacacc ttgtttttac agacaatgcg tctgttaaat tttttaaaca 28560ttgtgctcag tattgcttat gcctctggct atgcaaacat acagaaaacc ctctatgtag 28620gatctgatga tacactagag ggtacccaat cacaagctag ggtttcatgg tatttttata 28680aaagctcaga taatcctatt actctttgca aaggtgatca ggggcggaca acaaagccgc 28740ctatcacatt tagctgtacc agaacaaatc tcacgctttt ctcaattaca aaacaatatg 28800ctggtattta ttacagtaca aactttcata gtgggcaaga taaatattat actgttaagg 28860tagaaaatcc taccactcct agaactacca ccaccaccac caccaccacc actactgcga 28920agcccactaa acctaaaact accaagaaaa ccactgtgaa aactacaact agaaccacca 28980caactacaga aaccaccacc agcacaacac ttgctgcaac tacacacaca cacactgagc 29040taaccttaca gaccactaat gatttgatag ccctgttgca aaagggggat aacagcacca 29100cttccaatga ggagataccc aaatccatga ttggcattat tgttgctgta gtggtgtgca 29160tgttgatcat cgccttgtgc atggtgtact atgccttctg ctacagaaag cacagactga 29220acgacaagct ggaacactta ctaagtgttg aattttaatt ttttagaacc atgaagatcc 29280taggcctttt agttttttct atcattacct ctgctctatg caattctgac aatgaggacg 29340ttactgtcgt tgtcggatca aattatacac tgaaaggtcc agcgaagggt atgctttcgt 29400ggtattgctg gtttggaact gacactgatc aaactgagct ttgcaatgca atgaaaggtc 29460aaataccaac ctcaaaaatt aaacataaat gcaatggtac tgacttagta ctactcaata 29520tcacgaaatc atatgctggc agctattcat gccctggaga tgatgctgag aacatgattt 29580tttacaaagt aactgttgtt gatcccacta ctccaccacc caccaccaca actactcaca 29640ccacacacac agaacaaaca ccagaggcag cagaagcaga gttggccttc caggttcacg 29700gagattcctt tgctgtcaat acccctacac ccgatcatcg gtgtccgggg ctgctagtca 29760gcggcattgt cggtgtgctt tcgggattag cagtcataat catctgcatg ttcatttttg 29820cttgctgcta tagaaggctt taccgacaaa aatcagaccc actgctgaac ctctatgttt 29880aattttttcc agagccatga aggcagttag cgctctagtt ttttgttctt tgattggcat 29940tgttttttgc aatcctatta ctagagttag ctttattaaa gatgtgaatg ttactgaggg 30000gggcaatgtg acactggtag gtgtagaggg tgctaaaaac accacctgga caaaatacca 30060ccttgggtgg aaagatattt gcaattggag tgtcactgtg tacacatgtg agggagttaa 30120tcttaccatt gtcaatgcca cctcagctca aaatggtaga attcaaggac aaagtgttag 30180tgtgaccagt gatgggtatt ttacccaaca tacttttatc tatgacgtta aagtcatacc 30240actgcctacg cctagcccac ctagcaccac tacacaaaca acccacacta cacagacaac 30300cacatacagt acatcaaatc agcctaccac cactacagca gcagaggttg ccagctcgtc 30360tggagttcaa gtggcatttt tgttgttgcc cccatctagc agtcccactg ctattaccaa 30420tgagcagact actgcatttt tgtccactgt cgagagccac accacagcta cctccagtgc 30480cttctctagc accgccaatc tctcctcgct ttcctctaca ccaatcagtc ccgctactac 30540tactaccccc gctattcttc ccactcccct gaagcaaaca gacggcggca tgcaatggca 30600gatcaccctg ctcattgtga tcgggttggt catcctagcc gtgttgctct actacatctt 30660ctgccgccgc attcccaacg cgcaccgcaa gccggtctac aagcccatca ttgtcgggca 30720gccggagccg cttcaggtgg aagggggtct aaggaatctt ctcttctctt ttacagtatg 30780gtgattgaac tatgattcct agacaattct tgatcactat tcttatctgc ctcctccaag 30840tctgtgccac cctcgctctg gtggccaacg ccagtccaga ctgtattggg cccttcgcct 30900cctacgtgct ctttgccttc atcacctgca tctgctgctg tagcatagtc tgcctgctta 30960tcaccttctt ccagttcatt gactggatct ttgtgcgcat cgcctacctg cgccaccacc 31020cccagtaccg cgaccagcga gtggcgcagc tgctcaggct cctctgataa gcatgcgggc 31080tctgctactt ctcgcgcttc tgctgttagt gctcccccgt cccgttgacc cccggccccc 31140cactcagtcc cccgaggagg tccgcaaatg caaattccaa gaaccctgga aattcctcaa 31200atgctaccgc caaaaatcag acatgcatcc cagctggatc atgatcattg ggatcgtgaa 31260cattctggcc tgcaccctca tctcctttgt gatttacccc tgctttgact ttggttggaa 31320ctcgccagag gcgctctatc tcccgcctga acctgacaca ccaccacagc aacctcaggc 31380acacgcacta ccaccaccac agcctaggcc acaatacatg cccatattag actatgaggc 31440cgagccacag cgacccatgc tccccgctat tagttacttc aatctaaccg gcggagatga 31500ctgacccact ggccaacaac aacgtcaacg accttctcct ggacatggac ggccgcgcct 31560cggagcagcg actcgcccaa cttcgcattc gccagcagca ggagagagcc gtcaaggagc 31620tgcaggacgg catagccatc caccagtgca agaaaggcat cttctgcctg gtgaaacagg 31680ccaagatctc ctacgaggtc acccagaccg accatcgcct ctcctacgag ctcctgcagc 31740agcgccagaa gttcacctgc ctggtcggag tcaaccccat cgtcatcacc cagcagtcgg 31800gcgataccaa ggggtgcatc cactgctcct gcgactcccc cgactgcgtc cacactctga 31860tcaagaccct ctgcggcctc cgcgacctcc tccccatgaa ctaatcaccc acttatccag 31920tgaaataaaa aaataatcat ttgatttgaa ataaagatac aatcatattg atgatttgag 31980tttaacaaaa ataaagaatc acttacttga aatctgatac caggtctctg tccatatttt 32040ctgccaacac cacctcactc ccctcttccc agctctggta ctgcaggccc cggcgggctg 32100caaacttcct ccacacgctg aaggggatgt caaattcctc ctgcccctca atcttcattt 32160tatcttctat cagatgtcca aaaagcgcgt ccgggtggat gatgacttcg accccgtcta 32220cccctacgat gcagacaacg caccgaccgt gcccttcatc aaccccccct tcgtctcttc 32280agatggattc caagagaagc ccctgggggt gttgtccctg cgactggccg accccgtcac 32340caccaagaac ggggaaatca ccctcaagct gggagagggg gtggacctcg actcctcggg 32400aaaactcatc tccaacacgg ccaccaaggc cgctgcccct ctcagttttt ccaacaacac 32460catttccctt aacatggatc acccctttta cactaaagat ggaaaattag ccttacaagt 32520ttctccacca ttaaatatac tgagaacaag cattctaaac acactagctt taggttttgg 32580atcaggttta ggactccgtg gctctgcctt ggcagtacag ttagtctctc cacttacatt 32640tgatactgat ggaaacataa agcttacctt agacagaggt ttgcatgtta caacaggaga 32700tgcaattgaa agcaacataa gctgggctaa aggtttaaaa tttgaagatg gagccatagc 32760aaccaacatt ggaaatgggt tagagtttgg aagcagtagt acagaaacag gtgtcgatga 32820tgcttaccca atccaagtta aacttggatc tggccttagc tttgacagta caggagccat 32880aatggctggt aacaaagaag acgataaact cactttgtgg acaacacctg atccatcacc 32940aaactgtcaa atactcgcag aaaatgatgc aaaactaaca ctttgcttga ctaaatgtgg 33000tagtcaaata ctggccactg tgtcagtctt agttgtagga agtggaaacc taaaccccat 33060tactggcacc gtaagcagtg ctcaggtgtt tctacgtttt gatgcaaacg gtgttctttt 33120aacagaacat tctacactaa aaaaatactg ggggtatagg cagggagata gcatagatgg 33180cactccatat gtcaatgctg taggattcat gcccaattta aaagcttatc caaagtcaca 33240aagttctact actaaaaata atatagtagg gcaagtatac atgaatggag atgtttcaaa 33300acctatgctt ctcactataa ccctcaatgg tactgatgac agcaacagta catattcaat 33360gtcattttca tacacctgga ctaatggaag ctatgttgga gcaacatttg gagctaactc 33420ttataccttc tcctacatcg cccaagaatg aatactgtat cccaccctgc atgcccaacc 33480ctcccccacc tctgtctata tggaaaactc tgaaacacaa aataaaataa agttcaagtg 33540ttttattgat tcaacagttt tacaggattc gagcagttat ttttcctcca ccctcccagg 33600acatggaata caccaccctc tccccccgca cagccttgaa catctgaatg ccattggtga 33660tggacatgct tttggtctcc acgttccaca cagtttcaga gcgagccagt ctcgggtcgg 33720tcagggagat gaaaccctcc gggcactccc gcatctgcac ctcacagctc aacagctgag 33780gattgtcctc ggtggtcggg atcacggtta tctggaagaa gcagaagagc ggcggtggga 33840atcatagtcc gcgaacggga tcggccggtg gtgtcgcatc aggccccgca gcagtcgctg 33900ccgccgccgc tccgtcaagc tgctgctcag ggggtccggg tccagggact ccctcagcat 33960gatgcccacg gccctcagca tcagtcgtct ggtgcggcgg gcgcagcagc gcatgcggat 34020ctcgctcagg tcgctgcagt acgtgcaaca caggaccacc aggttgttca acagtccata 34080gttcaacacg ctccagccga aactcatcgc gggaaggatg ctacccacgt ggccgtcgta 34140ccagatcctc aggtaaatca agtggcgccc cctccagaac acgctgccca tgtacatgat 34200ctccttgggc atgtggcggt tcaccacctc ccggtaccac atcaccctct ggttgaacat 34260gcagccccgg atgatcctgc ggaaccacag ggccagcacc gccccgcccg ccatgcagcg 34320aagagacccc gggtcccggc aatggcaatg gaggacccac cgctcgtacc cgtggatcat 34380ctgggagctg aacaagtcta tgttggcaca gcacaggcac acgctcatgc atctcttcag 34440cactctcagc tcctcggggg tcaaaaccat atcccagggc acgggaaact cttgcaggac 34500agcgaagccc gcagaacagg gcaatcctcg cacataactt acattgtgca tggacagggt 34560atcgcaatca ggcagcaccg ggtgatcctc caccagagaa gcgcgggtct cggtctcctc 34620acagcgtggt aagggggccg gccgatacgg gtgatggcgg gacgcggctg atcgtgttcg 34680cgaccgtgtc atgatgcagt tgctttcgga cattttcgta cttgctgaag cagaacctgg 34740tccgggcgct gcacaccgat cgccggcggc ggtctcggcg cttggaacgc tcggtgttga 34800agttgtaaaa cagccactct ctcagaccgt gcagcagatc tagggcctca ggagtgatga 34860agatcccatc atgcctgatg gctctgatca catcgaccac cgtggaatgg gccagaccca 34920gccagatgat gcaattttgt tgggtttcgg tgacggcggg ggagggaaga acaggaagaa 34980ccatgattaa cttttaatcc aaacggtctc ggagcacttc aaaatgaagg tcgcggagat 35040ggcacctctc gcccccgctg tgttggtgga aaataacagc caggtcaaag gtgatacggt 35100tctcgagatg ttccacggtg gcttccagca aagcctccac gcgcacatcc agaaacaaga 35160caatagcgaa agcgggaggg ttctctaatt cctcaatcat catgttacac tcctgcacca 35220tccccagata attttcattt ttccagcctt gaatgattcg aactagttcc tgaggtaaat 35280ccaagccagc catgataaag agctcgcgca gagcgccctc caccggcatt cttaagcaca 35340ccctcataat tccaagatat tctgctcctg gttcacctgc agcagattga caagcgggat 35400atcaaaatct ctgccgcgat ccctgagctc ctccctcagc aataactgta agtactcttt 35460catatcctct ccgaaatttt tagccatagg acccccagga ataagagaag ggcaagccac 35520attacagata aaccgaagtc ccccccagtg agcattgcca aatgtaagat tgaaataagc 35580atgctggcta gacccggtga tatcttccag ataactggac agaaaatcgg gcaagcaatt 35640tttaagaaaa tcaacaaaag aaaaatcttc caggtgcacg tttagggcct cgggaacaac 35700gatggagtaa gtgcaagggg tgcgttccag catggttagt tagctgatct gtaaaaaaac 35760aaaaaataaa acattaaacc atgctagcct ggcgaacagg tgggtaaatc gttctctcca 35820gcaccaggca ggccacgggg tctccggcgc gaccctcgta aaaattgtcg ctatgattga 35880aaaccatcac agagagacgt tcccggtggc cggcgtgaat gattcgagaa gaagcataca 35940cccccggaac attggagtcc gtgagtgaaa aaaagcggcc gaggaagcaa tgaggcacta 36000caacgctcac tctcaagtcc agcaaagcga tgccatgcgg atgaagcaca aaattttcag 36060gtgcgtaaaa aatgtaatta ctcccctcct gcacaggcag cgaagctccc gatccctcca 36120gatacacata caaagcctca gcgtccatag cttaccgagc ggcagcagca gcggcacaca 36180acaggcgcaa gagtcagaga aaagactgag ctctaacctg tccgcccgct ctctgctcaa 36240tatatagccc cagatctaca ctgacgtaaa ggccaaagtc taaaaatacc cgccaaataa 36300tcacacacgc ccagcacacg cccagaaacc ggtgacacac tcaaaaaaat acgcgcactt 36360cctcaaacgc ccaaactgcc gtcatttccg ggttcccacg ctacgtcatc aaaacacgac 36420tttcaaattc cgtcgaccgt taaaaacgtc acccgccccg cccctaacgg tcgccgctcc 36480cgcagccaat cagcgccccg catccccaaa ttcaaacagc tcatttgcat attaacgcgc 36540accaaaagtt tgaggtatat tattgatgat g 3657131109DNAArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polynucleotide" 3ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 60ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 120ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 180tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 240tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 300cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc 360ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc 420gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg 480aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 540gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg ctccctatca gtgatagaga 600tctccctatc agtgatagag atcgtcgacg agctcgcggc gggcgggagt cgctgcgcgc 660tgccttcgcc ccgtgccccg ctccgccgcc

gcctcgcgcc gcccgccccg gctctgactg 720accgcgttac taaaacaggt aagtccggcc tccgcgccgg gttttggcgc ctcccgcggg 780cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga 840tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc 900ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt 960ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg 1020atctccgtgg ggcggtgaac gccgatgatg cctctactaa ccatgttcat gttttctttt 1080tttttctaca ggtcctgggt gacgaacag 1109437559DNAArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polynucleotide" 4catcatcaat aatatacctt attttggatt gaagccaata tgataatgag atgggcggcg 60cggggcgggg cgcggggcgg gaggcgggtt tgggggcggg ccggcgggcg gggcggtgtg 120gcggaagtgg actttgtaag tgtggcggat gtgacttgct agtgccgggc gcggtaaaag 180tgacgttttc cgtgcgcgac aacgcccccg ggaagtgaca tttttcccgc ggtttttacc 240ggatgttgta gtgaatttgg gcgtaaccaa gtaagatttg gccattttcg cgggaaaact 300gaaacgggga agtgaaatct gattaatttt gcgttagtca taccgcgtaa tatttgtcta 360gggccgaggg actttggccg attacgtgga ggactcgccc aggtgttttt tgaggtgaat 420ttccgcgttc cgggtcaaag tctgcgtttt attattatag gatatcccat tgcatacgtt 480gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg 540acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 600atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 660cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 720tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 780agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 840gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 900agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 960gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 1020gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 1080gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag 1140agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc 1200ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct 1260ccgcggccgg gaacggtgca ttggaacgcg gattccccgt gccaagagtg agatcttccg 1320tttatctagg taccagatat cgccaccatg gaactgctga tcctgaaggc caacgccatc 1380accaccatcc tgaccgccgt gaccttctgc ttcgccagcg gccagaacat caccgaggaa 1440ttctaccaga gcacctgtag cgccgtgagc aagggctacc tgagcgccct gagaaccggc 1500tggtacacca gcgtgatcac catcgagctg agcaacatca aagaaaacaa gtgcaacggc 1560accgacgcca aagtgaagct gatcaagcag gaactggaca agtacaagaa cgccgtgacc 1620gagctgcagc tgctgatgca gagcaccccc gccaccaaca accgggccag acgggagctg 1680ccccggttca tgaactacac cctgaacaac gccaaaaaga ccaacgtgac cctgagcaag 1740aagcggaagc ggcggttcct gggctttctg ctgggcgtgg gcagcgccat tgccagcggc 1800gtggccgtgt ctaaggtgct gcacctggaa ggcgaagtga acaagatcaa gagcgccctg 1860ctgagcacca acaaggccgt ggtgtccctg agcaacggcg tgagcgtgct gaccagcaag 1920gtgctggatc tgaagaacta catcgacaag cagctgctgc ccatcgtgaa caagcagagc 1980tgcagcatca gcaacatcga gacagtgatc gagttccagc agaagaacaa ccggctgctg 2040gaaatcaccc gggagttcag cgtgaacgcc ggcgtgacca cccctgtgtc cacctacatg 2100ctgaccaaca gcgagctgct gagcctgatc aacgacatgc ccatcaccaa cgaccagaaa 2160aagctgatga gcaacaacgt gcagatcgtg cggcagcaga gctactccat catgtccatc 2220atcaaagaag aggtgctggc ctacgtggtg cagctgcccc tgtacggcgt gatcgacacc 2280ccctgctgga agctgcacac cagccccctg tgcaccacca acaccaaaga gggcagcaac 2340atctgcctga cccggaccga cagaggctgg tactgcgaca acgccggcag cgtgtcattc 2400tttccacagg ccgagacatg caaggtgcag agcaaccggg tgttctgcga caccatgaac 2460agcctgaccc tgccctccga agtgaacctg tgcaacgtgg acatcttcaa ccccaagtac 2520gactgcaaga tcatgacctc caagaccgac gtgtccagct ccgtgatcac ctccctgggc 2580gccatcgtgt cctgctacgg caagaccaag tgcaccgcca gcaacaagaa ccggggcatc 2640atcaagacct tcagcaacgg ctgcgactac gtgtccaaca agggggtgga caccgtgtcc 2700gtgggcaaca ccctgtacta cgtgaacaaa caggaaggca agagcctgta cgtgaagggc 2760gagcccatca tcaacttcta cgaccccctg gtgttcccca gcgacgagtt cgacgccagc 2820atcagccagg tgaacgagaa gatcaaccag agcctggcct tcatccggaa gtccgacgag 2880ctgctgcaca atgtgaatgc cggcaagtcc accaccaacc ggaagcggag agcccctgtg 2940aagcagaccc tgaacttcga cctgctgaag ctggccggcg acgtggagag caatcccggc 3000cctatggccc tgagcaaagt gaaactgaac gatacactga acaaggacca gctgctgtcc 3060agcagcaagt acaccatcca gcggagcacc ggcgacagca tcgatacccc caactacgac 3120gtgcagaagc acatcaacaa gctgtgcggc atgctgctga tcacagagga cgccaaccac 3180aagttcaccg gcctgatcgg catgctgtac gccatgagcc ggctgggccg ggaggacacc 3240atcaagatcc tgcgggacgc cggctaccac gtgaaggcca atggcgtgga cgtgaccaca 3300caccggcagg acatcaacgg caaagaaatg aagttcgagg tgctgaccct ggccagcctg 3360accaccgaga tccagatcaa tatcgagatc gagagccgga agtcctacaa gaaaatgctg 3420aaagaaatgg gcgaggtggc ccccgagtac agacacgaca gccccgactg cggcatgatc 3480atcctgtgta tcgccgccct ggtgatcaca aagctggccg ctggcgacag atctggcctg 3540acagccgtga tcagacgggc caacaatgtg ctgaagaacg agatgaagcg gtacaagggc 3600ctgctgccca aggacattgc caacagcttc tacgaggtgt tcgagaagta cccccacttc 3660atcgacgtgt tcgtgcactt cggcattgcc cagagcagca ccagaggcgg ctccagagtg 3720gagggcatct tcgccggcct gttcatgaac gcctacggcg ctggccaggt gatgctgaga 3780tggggcgtgc tggccaagag cgtgaagaac atcatgctgg gccacgccag cgtgcaggcc 3840gagatggaac aggtggtgga ggtgtacgag tacgcccaga agctgggcgg agaggccggc 3900ttctaccaca tcctgaacaa ccctaaggcc tccctgctgt ccctgaccca gttcccccac 3960ttctccagcg tggtgctggg aaatgccgcc ggactgggca tcatgggcga gtaccggggc 4020acccccagaa accaggacct gtacgacgcc gccaaggcct acgccgagca gctgaaagaa 4080aacggcgtga tcaactacag cgtgctggac ctgaccgctg aggaactgga agccatcaag 4140caccagctga accccaagga caacgacgtg gagctgggag gcggaggatc tggcggcgga 4200ggcatgagca gacggaaccc ctgcaagttc gagatccggg gccactgcct gaacggcaag 4260cggtgccact tcagccacaa ctacttcgag tggccccctc atgctctgct ggtgcggcag 4320aacttcatgc tgaaccggat cctgaagtcc atggacaaga gcatcgacac cctgagcgag 4380atcagcggag ccgccgagct ggacagaacc gaggaatatg ccctgggcgt ggtgggagtg 4440ctggaaagct acatcggctc catcaacaac atcacaaagc agagcgcctg cgtggccatg 4500agcaagctgc tgacagagct gaacagcgac gacatcaaga agctgaggga caacgaggaa 4560ctgaacagcc ccaagatccg ggtgtacaac accgtgatca gctacattga gagcaaccgc 4620aagaacaaca agcagaccat ccatctgctg aagcggctgc ccgccgacgt gctgaaaaag 4680accatcaaga acaccctgga catccacaag tccatcacca tcaacaatcc caaagaaagc 4740accgtgtctg acaccaacga tcacgccaag aacaacgaca ccacctgatg agcggccgcg 4800atctgctgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt 4860gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca 4920ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 4980ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg ccgatcagcg 5040atcgctgagg tgggtgagtg ggcgtggcct ggggtggtca tgaaaatata taagttgggg 5100gtcttagggt ctctttattt gtgttgcaga gaccgccgga gccatgagcg ggagcagcag 5160cagcagcagt agcagcagcg ccttggatgg cagcatcgtg agcccttatt tgacgacgcg 5220gatgccccac tgggccgggg tgcgtcagaa tgtgatgggc tccagcatcg acggccgacc 5280cgtcctgccc gcaaattccg ccacgctgac ctatgcgacc gtcgcgggga cgccgttgga 5340cgccaccgcc gccgccgccg ccaccgcagc cgcctcggcc gtgcgcagcc tggccacgga 5400ctttgcattc ctgggaccac tggcgacagg ggctacttct cgggccgctg ctgccgccgt 5460tcgcgatgac aagctgaccg ccctgctggc gcagttggat gcgcttactc gggaactggg 5520tgacctttct cagcaggtca tggccctgcg ccagcaggtc tcctccctgc aagctggcgg 5580gaatgcttct cccacaaatg ccgtttaaga taaataaaac cagactctgt ttggattaaa 5640gaaaagtagc aagtgcattg ctctctttat ttcataattt tccgcgcgcg ataggcccta 5700gaccagcgtt ctcggtcgtt gagggtgcgg tgtatcttct ccaggacgtg gtagaggtgg 5760ctctggacgt tgagatacat gggcatgagc ccgtcccggg ggtggaggta gcaccactgc 5820agagcttcat gctccggggt ggtgttgtag atgatccagt cgtagcagga gcgctgggca 5880tggtgcctaa aaatgtcctt cagcagcagg ccgatggcca gggggaggcc cttggtgtaa 5940gtgtttacaa aacggttaag ttgggaaggg tgcattcggg gagagatgat gtgcatcttg 6000gactgtattt ttagattggc gatgtttccg cccagatccc ttctgggatt catgttgtgc 6060aggaccacca gtacagtgta tccggtgcac ttggggaatt tgtcatgcag cttagaggga 6120aaagcgtgga agaacttgga gacgcctttg tggcctccca gattttccat gcattcgtcc 6180atgatgatgg caatgggccc gcgggaggca gcttgggcaa agatatttct ggggtcgctg 6240acgtcgtagt tgtgttccag ggtgaggtcg tcataggcca tttttacaaa gcgcgggcgg 6300agggtgcccg actgggggat gatggtcccc tctggccctg gggcgtagtt gccctcgcag 6360atctgcattt cccaggcctt aatctcggag gggggaatca tatccacctg cggggcgatg 6420aagaaaacgg tttccggagc cggggagatt aactgggatg agagcaggtt tctaagcagc 6480tgtgattttc cacaaccggt gggcccataa ataacaccta taaccggttg cagctggtag 6540tttagagagc tgcagctgcc gtcgtcccgg aggagggggg ccacctcgtt gagcatgtcc 6600ctgacgcgca tgttctcccc gaccagatcc gccagaaggc gctcgccgcc cagggacagc 6660agctcttgca aggaagcaaa gtttttcagc ggcttgaggc cgtccgccgt gggcatgttt 6720ttcagggtct ggctcagcag ctccaggcgg tcccagagct cggtgacgtg ctctacggca 6780tctctatcca gcatatctcc tcgtttcgcg ggttggggcg actttcgctg tagggcacca 6840agcggtggtc gtccagcggg gccagagtca tgtccttcca tgggcgcagg gtcctcgtca 6900gggtggtctg ggtcacggtg aaggggtgcg ctccgggctg agcgcttgcc aaggtgcgct 6960tgaggctggt tctgctggtg ctgaagcgct gccggtcttc gccctgcgcg tcggccaggt 7020agcatttgac catggtgtca tagtccagcc cctccgcggc gtgtcccttg gcgcgcagct 7080tgcccttgga ggtggcgccg cacgaggggc agagcaggct cttgagcgcg tagagcttgg 7140gggcgaggaa gaccgattcg ggggagtagg cgtccgcgcc gcagaccccg cacacggtct 7200cgcactccac cagccaggtg agctcggggc gcgccgggtc aaaaaccagg tttcccccat 7260gctttttgat gcgtttctta cctcgggtct ccatgaggtg gtgtccccgc tcggtgacga 7320agaggctgtc cgtgtctccg tagaccgact tgaggggtct tttctccagg ggggtccctc 7380ggtcttcctc gtagaggaac tcggaccact ctgagacgaa ggcccgcgtc caggccagga 7440cgaaggaggc tatgtgggag gggtagcggt cgttgtccac tagggggtcc accttctcca 7500aggtgtgaag acacatgtcg ccttcctcgg cgtccaggaa ggtgattggc ttgtaggtgt 7560aggccacgtg accgggggtt cctgacgggg gggtataaaa gggggtgggg gcgcgctcgt 7620cgtcactctc ttccgcatcg ctgtctgcga gggccagctg ctggggtgag tattccctct 7680cgaaggcggg catgacctcc gcgctgaggt tgtcagtttc caaaaacgag gaggatttga 7740tgttcacctg tcccgaggtg atacctttga gggtacccgc gtccatctgg tcagaaaaca 7800cgatcttttt attgtccagc ttggtggcga acgacccgta gagggcgttg gagagcagct 7860tggcgatgga gcgcagggtc tggttcttgt ccctgtcggc gcgctccttg gccgcgatgt 7920tgagctgcac gtactcgcgc gcgacgcagc gccactcggg gaagacggtg gtgcgctcgt 7980cgggcaccag gcgcacgcgc cagccgcggt tgtgcagggt gaccaggtcc acgctggtgg 8040cgacctcgcc gcgcaggcgc tcgttggtcc agcagagacg gccgcccttg cgcgagcaga 8100aggggggcag ggggtcgagc tgggtctcgt ccggggggtc cgcgtccacg gtgaaaaccc 8160cggggcgcag gcgcgcgtcg aagtagtcta tcttgcaacc ttgcatgtcc agcgcctgct 8220gccagtcgcg ggcggcgagc gcgcgctcgt aggggttgag cggcgggccc cagggcatgg 8280ggtgggtgag tgcggaggcg tacatgccgc agatgtcata gacgtagagg ggctcccgca 8340ggaccccgat gtaggtgggg tagcagcggc cgccgcggat gctggcgcgc acgtagtcat 8400acagctcgtg cgagggggcg aggaggtcgg ggcccaggtt ggtgcgggcg gggcgctccg 8460cgcggaagac gatctgcctg aagatggcat gcgagttgga agagatggtg gggcgctgga 8520agacgttgaa gctggcgtcc tgcaggccga cggcgtcgcg cacgaaggag gcgtaggagt 8580cgcgcagctt gtgtaccagc tcggcggtga cctgcacgtc gagcgcgcag tagtcgaggg 8640tctcgcggat gatgtcatat ttagcctgcc ccttcttttt ccacagctcg cggttgagga 8700caaactcttc gcggtctttc cagtactctt ggatcgggaa accgtccggt tccgaacggt 8760aagagcctag catgtagaac tggttgacgg cctggtaggc gcagcagccc ttctccacgg 8820ggagggcgta ggcctgcgcg gccttgcgga gcgaggtgtg ggtcagggcg aaggtgtccc 8880tgaccatgac tttgaggtac tggtgcttga agtcggagtc gtcgcagccg ccccgctccc 8940agagcgagaa gtcggtgcgc ttcttggagc gggggttggg cagagcgaag gtgacatcgt 9000tgaagaggat tttgcccgcg cggggcatga agttgcgggt gatgcggaag ggccccggca 9060cttcagagcg gttgttgatg acctgggcgg cgagcacgat ctcgtcgaag ccgttgatgt 9120tgtggcccac gatgtagagt tccaggaagc ggggccggcc ctttacggtg ggcagcttct 9180ttagctcttc gtaggtgagc tcctcgggcg aggcgaggcc gtgctcggcc agggcccagt 9240ccgcgaggtg cgggttgtct ctgaggaagg acttccagag gtcgcgggcc aggagggtct 9300gcaggcggtc tctgaaggtc ctgaactggc ggcccacggc cattttttcg ggggtgatgc 9360agtagaaggt gagggggtct tgctgccagc ggtcccagtc gagctgcagg gcgaggtcgc 9420gcgcggcggt gaccaggcgc tcgtcgcccc cgaatttcat gaccagcatg aagggcacga 9480gctgctttcc gaaggccccc atccaagtgt aggtctctac atcgtaggtg acaaagaggc 9540gctccgtgcg aggatgcgag ccgatcggga agaactggat ctcccgccac cagttggagg 9600agtggctgtt gatgtggtgg aagtagaagt cccgtcgccg ggccgaacac tcgtgctggc 9660ttttgtaaaa gcgagcgcag tactggcagc gctgcacggg ctgtacctca tgcacgagat 9720gcacctttcg cccgcgcacg aggaagccga ggggaaatct gagccccccg cctggctcgc 9780ggcatggctg gttctcttct actttggatg cgtgtccgtc tccgtctggc tcctcgaggg 9840gtgttacggt ggagcggacc accacgccgc gcgagccgca ggtccagata tcggcgcgcg 9900gcggtcggag tttgatgacg acatcgcgca gctgggagct gtccatggtc tggagctccc 9960gcggcggcgg caggtcagcc gggagttctt gcaggttcac ctcgcagagt cgggccaggg 10020cgcggggcag gtctaggtgg tacctgatct ctaggggcgt gttggtggcg gcgtcgatgg 10080cttgcaggag cccgcagccc cggggggcga cgacggtgcc ccgcggggtg gtggtggtgg 10140tggcggtgca gctcagaagc ggtgccgcgg gcgggccccc ggaggtaggg ggggctccgg 10200tcccgcgggc aggggcggca gcggcacgtc ggcgtggagc gcgggcagga gttggtgctg 10260tgcccggagg ttgctggcga aggcgacgac gcggcggttg atctcctgga tctggcgcct 10320ctgcgtgaag acgacgggcc cggtgagctt gaacctgaaa gagagttcga cagaatcaat 10380ctcggtgtca ttgaccgcgg cctggcgcag gatctcctgc acgtctcccg agttgtcttg 10440gtaggcgatc tcggccatga actgctcgat ctcttcctcc tggaggtctc cgcgtccggc 10500gcgttccacg gtggccgcca ggtcgttgga gatgcgcccc atgagctgcg agaaggcgtt 10560gagtccgccc tcgttccaga ctcggctgta gaccacgccc ccctggtcat cgcgggcgcg 10620catgaccacc tgcgcgaggt tgagctccac gtgccgcgcg aagacggcgt agttgcgcag 10680acgctggaag aggtagttga gggtggtggc ggtgtgctcg gccacgaaga agttcatgac 10740ccagcggcgc aacgtggatt cgttgatgtc ccccaaggcc tccagccgtt ccatggcctc 10800gtagaagtcc acggcgaagt tgaaaaactg ggagttgcgc gccgacacgg tcaactcctc 10860ctccagaaga cggatgagct cggcgacggt gtcgcgcacc tcgcgctcga aggctatggg 10920gatctcttcc tccgctagca tcaccacctc ctcctcttcc tcctcttctg gcacttccat 10980gatggcttcc tcctcttcgg ggggtggcgg cggcggcggt gggggagggg gcgctctgcg 11040ccggcggcgg cgcaccggga ggcggtccac gaagcgcgcg atcatctccc cgcggcggcg 11100gcgcatggtc tcggtgacgg cgcggccgtt ctcccggggg cgcagttgga agacgccgcc 11160ggacatctgg tgctggggcg ggtggccgtg aggcagcgag acggcgctga cgatgcatct 11220caacaattgc tgcgtaggta cgccgccgag ggacctgagg gagtccatat ccaccggatc 11280cgaaaacctt tcgaggaagg cgtctaacca gtcgcagtcg caaggtaggc tgagcaccgt 11340ggcgggcggc ggggggtggg gggagtgtct ggcggaggtg ctgctgatga tgtaattgaa 11400gtaggcggac ttgacacggc ggatggtcga caggagcacc atgtccttgg gtccggcctg 11460ctggatgcgg aggcggtcgg ctatgcccca ggcttcgttc tggcatcggc gcaggtcctt 11520gtagtagtct tgcatgagcc tttccaccgg cacctcttct ccttcctctt ctgcttcttc 11580catgtctgct tcggccctgg ggcggcgccg cgcccccctg ccccccatgc gcgtgacccc 11640gaaccccctg agcggttgga gcagggccag gtcggcgacg acgcgctcgg ccaggatggc 11700ctgctgcacc tgcgtgaggg tggtttggaa gtcatccaag tccacgaagc ggtggtaggc 11760gcccgtgttg atggtgtagg tgcagttggc catgacggac cagttgacgg tctggtggcc 11820cggttgcgac atctcggtgt acctgagtcg cgagtaggcg cgggagtcga agacgtagtc 11880gttgcaagtc cgcaccaggt actggtagcc caccaggaag tgcggcggcg gctggcggta 11940gaggggccag cgcagggtgg cgggggctcc gggggccagg tcttccagca tgaggcggtg 12000gtaggcgtag atgtacctgg acatccaggt gatacccgcg gcggtggtgg aggcgcgcgg 12060gaagtcgcgc acccggttcc agatgttgcg caggggcaga aagtgctcca tggtaggcgt 12120gctctgtcca gtcagacgcg cgcagtcgtt gatactctag accagggaaa acgaaagccg 12180gtcagcgggc actcttccgt ggtctggtga atagatcgca agggtatcat ggcggagggc 12240ctcggttcga gccccgggtc cgggccggac ggtccgccat gatccacgcg gttaccgccc 12300gcgtgtcgaa cccaggtgtg cgacgtcaga caacggtgga gtgttccttt tggcgttttt 12360ctggccgggc gccggcgccg cgtaagagac taagccgcga aagcgaaagc agtaagtggc 12420tcgctccccg tagccggagg gatccttgct aagggttgcg ttgcggcgaa ccccggttcg 12480aatcccgtac tcgggccggc cggacccgcg gctaaggtgt tggattggcc tccccctcgt 12540ataaagaccc cgcttgcgga ttgactccgg acacggggac gagccccttt tatttttgct 12600ttccccagat gcatccggtg ctgcggcaga tgcgcccccc gccccagcag cagcaacaac 12660accagcaaga gcggcagcaa cagcagcggg agtcatgcag ggccccctca cccaccctcg 12720gcgggccggc cacctcggcg tccgcggccg tgtctggcgc ctgcggcggc ggcggggggc 12780cggctgacga ccccgaggag cccccgcggc gcagggccag acactacctg gacctggagg 12840agggcgaggg cctggcgcgg ctgggggcgc cgtctcccga gcgccacccg cgggtgcagc 12900tgaagcgcga ctcgcgcgag gcgtacgtgc ctcggcagaa cctgttcagg gaccgcgcgg 12960gcgaggagcc cgaggagatg cgggacagga ggttcagcgc agggcgggag ctgcggcagg 13020ggctgaaccg cgagcggctg ctgcgcgagg aggactttga gcccgacgcg cggacgggga 13080tcagccccgc gcgcgcgcac gtggcggccg ccgacctggt gacggcgtac gagcagacgg 13140tgaaccagga gatcaacttc caaaagagtt tcaacaacca cgtgcgcacg ctggtggcgc 13200gcgaggaggt gaccatcggg ctgatgcacc tgtgggactt tgtaagcgcg ctggtgcaga 13260accccaacag caagcctctg acggcgcagc tgttcctgat agtgcagcac agcagggaca 13320acgaggcgtt tagggacgcg ctgctgaaca tcaccgagcc cgagggtcgg tggctgctgg 13380acctgattaa catcctgcag agcatagtgg tgcaggagcg cagcctgagc ctggccgaca 13440aggtggcggc catcaactac tcgatgctga gcctgggcaa gttttacgcg cgcaagatct 13500accagacgcc gtacgtgccc atagacaagg aggtgaagat cgacggtttt tacatgcgca 13560tggcgctgaa ggtgctcacc ctgagcgacg acctgggcgt gtaccgcaac gagcgcatcc 13620acaaggccgt gagcgtgagc cggcggcgcg agctgagcga ccgcgagctg atgcacagcc 13680tgcagcgggc gctggcgggc gccggcagcg gcgacaggga ggcggagtcc tacttcgatg 13740cgggggcgga cctgcgctgg gcgcccagcc ggcgggccct ggaggccgcg ggggtccgcg 13800aggactatga cgaggacggc gaggaggatg aggagtacga gctagaggag ggcgagtacc 13860tggactaaac cgcgggtggt gtttccggta gatgcaagac ccgaacgtgg tggacccggc 13920gctgcgggcg gctctgcaga gccagccgtc cggccttaac tcctcagacg actggcgaca 13980ggtcatggac cgcatcatgt cgctgacggc gcgtaacccg gacgcgttcc ggcagcagcc 14040gcaggccaac aggctctccg ccatcctgga ggcggtggtg cctgcgcgct cgaaccccac 14100gcacgagaag gtgctggcca tagtgaacgc gctggccgag aacagggcca tccgcccgga 14160cgaggccggg ctggtgtacg acgcgctgct gcagcgcgtg gcccgctaca acagcggcaa 14220cgtgcagacc aacctggacc ggctggtggg ggacgtgcgc gaggcggtgg cgcagcgcga 14280gcgcgcggat cggcagggca acctgggctc catggtggcg ctgaatgcct tcctgagcac 14340gcagccggcc aacgtgccgc gggggcagga agactacacc aactttgtga gcgcgctgcg 14400gctgatggtg accgagaccc cccagagcga ggtgtaccag tcgggcccgg actacttctt 14460ccagaccagc agacagggcc tgcagacggt gaacctgagc caggctttca

agaacctgcg 14520ggggctgtgg ggcgtgaagg cgcccaccgg cgaccgggcg acggtgtcca gcctgctgac 14580gcccaactcg cgcctgctgc tgctgctgat cgcgccgttc acggacagcg gcagcgtgtc 14640ccgggacacc tacctggggc acctgctgac cctgtaccgc gaggccatcg ggcaggcgca 14700ggtggacgag cacaccttcc aggagatcac cagcgtgagc cgcgcgctgg ggcaggagga 14760cacgagcagc ctggaggcga ctctgaacta cctgctgacc aaccggcggc agaagattcc 14820ctcgctgcac agcctgacct ccgaggagga gcgcatcttg cgctacgtgc agcagagcgt 14880gagcctgaac ctgatgcgcg acggggtgac gcccagcgtg gcgctggaca tgaccgcgcg 14940caacatggaa ccgggcatgt acgccgcgca ccggccttac atcaaccgcc tgatggacta 15000cctgcatcgc gcggcggccg tgaaccccga gtactttacc aacgccatcc tgaacccgca 15060ctggctcccg ccgcccgggt tctacagcgg gggcttcgag gtcccggaga ccaacgatgg 15120cttcctgtgg gacgacatgg acgacagcgt gttctccccg cggccgcagg cgctggcgga 15180agcgtccctg ctgcgtccca agaaggagga ggaggaggag gcgagtcgcc gccgcggcag 15240cagcggcgtg gcttctctgt ccgagctggg ggcggcagcc gccgcgcgcc ccgggtccct 15300gggcggcagc ccctttccga gcctggtggg gtctctgcac agcgagcgca ccacccgccc 15360tcggctgctg ggcgaggacg agtacctgaa taactccctg ctgcagccgg tgcgggagaa 15420aaacctgcct cccgccttcc ccaacaacgg gatagagagc ctggtggaca agatgagcag 15480atggaagacc tatgcgcagg agcacaggga cgcgcctgcg ctccggccgc ccacgcggcg 15540ccagcgccac gaccggcagc gggggctggt gtgggatgac gaggactccg cggacgatag 15600cagcgtgctg gacctgggag ggagcggcaa cccgttcgcg cacctgcgcc cccgcctggg 15660gaggatgttt taaaaaaaaa aaaaaaaagc aagaagcatg atgcaaaaat taaataaaac 15720tcaccaaggc catggcgacc gagcgttggt ttcttgtgtt cccttcagta tgcggcgcgc 15780ggcgatgtac caggagggac ctcctccctc ttacgagagc gtggtgggcg cggcggcggc 15840ggcgccctct tctccctttg cgtcgcagct gctggagccg ccgtacgtgc ctccgcgcta 15900cctgcggcct acggggggga gaaacagcat ccgttactcg gagctggcgc ccctgttcga 15960caccacccgg gtgtacctgg tggacaacaa gtcggcggac gtggcctccc tgaactacca 16020gaacgaccac agcaattttt tgaccacggt catccagaac aatgactaca gcccgagcga 16080ggccagcacc cagaccatca atctggatga ccggtcgcac tggggcggcg acctgaaaac 16140catcctgcac accaacatgc ccaacgtgaa cgagttcatg ttcaccaata agttcaaggc 16200gcgggtgatg gtgtcgcgct cgcacaccaa ggaagaccgg gtggagctga agtacgagtg 16260ggtggagttc gagctgccag agggcaacta ctccgagacc atgaccattg acctgatgaa 16320caacgcgatc gtggagcact atctgaaagt gggcaggcag aacggggtcc tggagagcga 16380catcggggtc aagttcgaca ccaggaactt ccgcctgggg ctggaccccg tgaccgggct 16440ggttatgccc ggggtgtaca ccaacgaggc cttccatccc gacatcatcc tgctgcccgg 16500ctgcggggtg gacttcactt acagccgcct gagcaacctc ctgggcatcc gcaagcggca 16560gcccttccag gagggcttca ggatcaccta cgaggacctg gaggggggca acatccccgc 16620gctcctcgat gtggaggcct accaggatag cttgaaggaa aatgaggcgg gacaggagga 16680taccgccccc gccgcctccg ccgccgccga gcagggcgag gatgctgctg acaccgcggc 16740cgcggacggg gcagaggccg accccgctat ggtggtggag gctcccgagc aggaggagga 16800catgaatgac agtgcggtgc gcggagacac cttcgtcacc cggggggagg aaaagcaagc 16860ggaggccgag gccgcggccg aggaaaagca actggcggca gcagcggcgg cggcggcgtt 16920ggccgcggcg gaggctgagt ctgaggggac caagcccgcc aaggagcccg tgattaagcc 16980cctgaccgaa gatagcaaga agcgcagtta caacctgctc aaggacagca ccaacaccgc 17040gtaccgcagc tggtacctgg cctacaacta cggcgacccg tcgacggggg tgcgctcctg 17100gaccctgctg tgcacgccgg acgtgacctg cggctcggag caggtgtact ggtcgctgcc 17160cgacatgatg caagaccccg tgaccttccg ctccacgcgg caggtcagca acttcccggt 17220ggtgggcgcc gagctgctgc ccgtgcactc caagagcttc tacaacgacc aggccgtcta 17280ctcccagctc atccgccagt tcacctctct gacccacgtg ttcaatcgct ttcctgagaa 17340ccagattctg gcgcgcccgc ccgcccccac catcaccacc gtcagtgaaa acgttcctgc 17400tctcacagat cacgggacgc taccgctgcg caacagcatc ggaggagtcc agcgagtgac 17460cgttactgac gccagacgcc gcacctgccc ctacgtttac aaggccttgg gcatagtctc 17520gccgcgcgtc ctttccagcc gcactttttg agcaacacca ccatcatgtc catcctgatc 17580tcacccagca ataactccgg ctggggactg ctgcgcgcgc ccagcaagat gttcggaggg 17640gcgaggaagc gttccgagca gcaccccgtg cgcgtgcgcg ggcacttccg cgccccctgg 17700ggagcgcaca aacgcggccg cgcggggcgc accaccgtgg acgacgccat cgactcggtg 17760gtggagcagg cgcgcaacta caggcccgcg gtctctaccg tggacgcggc catccagacc 17820gtggtgcggg gcgcgcggcg gtacgccaag ctgaagagcc gccggaagcg cgtggcccgc 17880cgccaccgcc gccgacccgg ggccgccgcc aaacgcgccg ccgcggccct gcttcgccgg 17940gccaagcgca cgggccgccg cgccgccatg agggccgcgc gccgcttggc cgccggcatc 18000accgccgcca ccatggcccc ccgtacccga agacgcgcgg ccgccgccgc cgccgccgcc 18060atcagtgaca tggccagcag gcgccggggc aacgtgtact gggtgcgcga ctcggtgacc 18120ggcacgcgcg tgcccgtgcg cttccgcccc ccgcggactt gagatgatgt gaaaaaacaa 18180cactgagtct cctgctgttg tgtgtatccc agcggcggcg gcgcgcgcag cgtcatgtcc 18240aagcgcaaaa tcaaagaaga gatgctccag gtcgtcgcgc cggagatcta tgggcccccg 18300aagaaggaag agcaggattc gaagccccgc aagataaagc gggtcaaaaa gaaaaagaaa 18360gatgatgacg atgccgatgg ggaggtggag ttcctgcgcg ccacggcgcc caggcgcccg 18420gtgcagtgga agggccggcg cgtaaagcgc gtcctgcgcc ccggcaccgc ggtggtcttc 18480acgcccggcg agcgctccac ccggactttc aagcgcgtct atgacgaggt gtacggcgac 18540gaagacctgc tggagcaggc caacgagcgc ttcggagagt ttgcttacgg gaagcgtcag 18600cgggcgctgg ggaaggagga cctgctggcg ctgccgctgg accagggcaa ccccaccccc 18660agtctgaagc ccgtgaccct gcagcaggtg ctgccgagca gcgcaccctc cgaggcgaag 18720cggggtctga agcgcgaggg cggcgacctg gcgcccaccg tgcagctcat ggtgcccaag 18780cggcagaggc tggaggatgt gctggagaaa atgaaagtag accccggtct gcagccggac 18840atcagggtcc gccccatcaa gcaggtggcg ccgggcctcg gcgtgcagac cgtggacgtg 18900gtcatcccca ccggcaactc ccccgccgcc gccaccacta ccgctgcctc cacggacatg 18960gagacacaga ccgatcccgc cgcagccgca gccgcagccg ccgccgcgac ctcctcggcg 19020gaggtgcaga cggacccctg gctgccgccg gcgatgtcag ctccccgcgc gcgtcgcggg 19080cgcaggaagt acggcgccgc caacgcgctc ctgcccgagt acgccttgca tccttccatc 19140gcgcccaccc ccggctaccg aggctatacc taccgcccgc gaagagccaa gggttccacc 19200cgccgtcccc gccgacgcgc cgccgccacc acccgccgcc gccgccgcag acgccagccc 19260gcactggctc cagtctccgt gaggaaagtg gcgcgcgacg gacacaccct ggtgctgccc 19320agggcgcgct accaccccag catcgtttaa aagcctgttg tggttcttgc agatatggcc 19380ctcacttgcc gcctccgttt cccggtgccg ggataccgag gaggaagatc gcgccgcagg 19440aggggtctgg ccggccgcgg cctgagcgga ggcagccgcc gcgcgcaccg gcggcgacgc 19500gccaccagcc gacgcatgcg cggcggggtg ctgcccctgt taatccccct gatcgccgcg 19560gcgatcggcg ccgtgcccgg gatcgcctcc gtggccttgc aagcgtccca gaggcattga 19620cagacttgca aacttgcaaa tatggaaaaa aaaaccccaa taaaaaagtc tagactctca 19680cgctcgcttg gtcctgtgac tattttgtag aatggaagac atcaactttg cgtcgctggc 19740cccgcgtcac ggctcgcgcc cgttcctggg acactggaac gatatcggca ccagcaacat 19800gagcggtggc gccttcagtt ggggctctct gtggagcggc attaaaagta tcgggtctgc 19860cgttaaaaat tacggctccc gggcctggaa cagcagcacg ggccagatgt tgagagacaa 19920gttgaaagag cagaacttcc agcagaaggt ggtggagggc ctggcctccg gcatcaacgg 19980ggtggtggac ctggccaacc aggccgtgca gaataagatc aacagcagac tggacccccg 20040gccgccggtg gaggaggtgc cgccggcgct ggagacggtg tcccccgatg ggcgtggcga 20100gaagcgcccg cggcccgata gggaagagac cactctggtc acgcagaccg atgagccgcc 20160cccgtatgag gaggccctga agcaaggtct gcccaccacg cggcccatcg cgcccatggc 20220caccggggtg gtgggccgcc acacccccgc cacgctggac ttgcctccgc ccgccgatgt 20280gccgcagcag cagaaggcgg cacagccggg cccgcccgcg accgcctccc gttcctccgc 20340cggtcctctg cgccgcgcgg ccagcggccc ccgcgggggg gtcgcgaggc acggcaactg 20400gcagagcacg ctgaacagca tcgtgggtct gggggtgcgg tccgtgaagc gccgccgatg 20460ctactgaata gcttagctaa cgtgttgtat gtgtgtatgc gccctatgtc gccgccagag 20520gagctgctga gtcgccgccg ttcgcgcgcc caccaccacc gccactccgc ccctcaagat 20580ggcgacccca tcgatgatgc cgcagtggtc gtacatgcac atctcgggcc aggacgcctc 20640ggagtacctg agccccgggc tggtgcagtt cgcccgcgcc accgagagct acttcagcct 20700gagtaacaag tttaggaacc ccacggtggc gcccacgcac gatgtgacca ccgaccggtc 20760tcagcgcctg acgctgcggt tcattcccgt ggaccgcgag gacaccgcgt actcgtacaa 20820ggcgcggttc accctggccg tgggcgacaa ccgcgtgctg gacatggcct ccacctactt 20880tgacatccgc ggggtgctgg accggggtcc cactttcaag ccctactctg gcaccgccta 20940caactccctg gcccccaagg gcgctcccaa ctcctgcgag tgggagcaag aggaaactca 21000ggcagttgaa gaagcagcag aagaggaaga agaagatgct gacggtcaag ctgaggaaga 21060gcaagcagct accaaaaaga ctcatgtata tgctcaggct cccctttctg gcgaaaaaat 21120tagtaaagat ggtctgcaaa taggaacgga cgctacagct acagaacaaa aacctattta 21180tgcagaccct acattccagc ccgaacccca aatcggggag tcccagtgga atgaggcaga 21240tgctacagtc gccggcggta gagtgctaaa gaaatctact cccatgaaac catgctatgg 21300ttcctatgca agacccacaa atgctaatgg aggtcagggt gtactaacgg caaatgccca 21360gggacagcta gaatctcagg ttgaaatgca attcttttca acttctgaaa acgcccgtaa 21420cgaggctaac aacattcagc ccaaattggt gctgtatagt gaggatgtgc acatggagac 21480cccggatacg cacctttctt acaagcccgc aaaaagcgat gacaattcaa aaatcatgct 21540gggtcagcag tccatgccca acagacctaa ttacatcggc ttcagagaca actttatcgg 21600cctcatgtat tacaatagca ctggcaacat gggagtgctt gcaggtcagg cctctcagtt 21660gaatgcagtg gtggacttgc aagacagaaa cacagaactg tcctaccagc tcttgcttga 21720ttccatgggt gacagaacca gatacttttc catgtggaat caggcagtgg acagttatga 21780cccagatgtt agaattattg aaaatcatgg aactgaagac gagctcccca actattgttt 21840ccctctgggt ggcatagggg taactgacac ttaccaggct gttaaaacca acaatggcaa 21900taacgggggc caggtgactt ggacaaaaga tgaaactttt gcagatcgca atgaaatagg 21960ggtgggaaac aatttcgcta tggagatcaa cctcagtgcc aacctgtgga gaaacttcct 22020gtactccaac gtggcgctgt acctaccaga caagcttaag tacaacccct ccaatgtgga 22080catctctgac aaccccaaca cctacgatta catgaacaag cgagtggtgg ccccggggct 22140ggtggactgc tacatcaacc tgggcgcgcg ctggtcgctg gactacatgg acaacgtcaa 22200ccccttcaac caccaccgca atgcgggcct gcgctaccgc tccatgctcc tgggcaacgg 22260gcgctacgtg cccttccaca tccaggtgcc ccagaagttc tttgccatca agaacctcct 22320cctcctgccg ggctcctaca cctacgagtg gaacttcagg aaggatgtca acatggtcct 22380ccagagctct ctgggtaacg atctcagggt ggacggggcc agcatcaagt tcgagagcat 22440ctgcctctac gccaccttct tccccatggc ccacaacacg gcctccacgc tcgaggccat 22500gctcaggaac gacaccaacg accagtcctt caatgactac ctctccgccg ccaacatgct 22560ctaccccata cccgccaacg ccaccaacgt ccccatctcc atcccctcgc gcaactgggc 22620ggccttccgc ggctgggcct tcacccgcct caagaccaag gagaccccct ccctgggctc 22680gggattcgac ccctactaca cctactcggg ctccattccc tacctggacg gcaccttcta 22740cctcaaccac actttcaaga aggtctcggt caccttcgac tcctcggtca gctggccggg 22800caacgaccgt ctgctcaccc ccaacgagtt cgagatcaag cgctcggtcg acggggaggg 22860ctacaacgtg gcccagtgca acatgaccaa ggactggttc ctggtccaga tgctggccaa 22920ctacaacatc ggctaccagg gcttctacat cccagagagc tacaaggaca ggatgtactc 22980cttcttcagg aacttccagc ccatgagccg gcaggtggtg gaccagacca agtacaagga 23040ctaccaggag gtgggcatca tccaccagca caacaactcg ggcttcgtgg gctacctcgc 23100ccccaccatg cgcgagggac aggcctaccc cgccaacttc ccctatccgc tcataggcaa 23160gaccgcggtc gacagcatca cccagaaaaa gttcctctgc gaccgcaccc tctggcgcat 23220ccccttctcc agcaacttca tgtccatggg tgcgctctcg gacctgggcc agaacttgct 23280ctacgccaac tccgcccacg ccctcgacat gaccttcgag gtcgacccca tggacgagcc 23340cacccttctc tatgttctgt tcgaagtctt tgacgtggtc cgggtccacc agccgcaccg 23400cggcgtcatc gagaccgtgt acctgcgtac gcccttctcg gccggcaacg ccaccaccta 23460aagaagcaag ccgcagtcat cgccgcctgc atgccgtcgg gttccaccga gcaagagctc 23520agggccatcg tcagagacct gggatgcggg ccctattttt tgggcacctt cgacaagcgc 23580ttccctggct ttgtctcccc acacaagctg gcctgcgcca tcgtcaacac ggccggccgc 23640gagaccgggg gcgtgcactg gctggccttc gcctggaacc cgcgctccaa aacatgcttc 23700ctctttgacc ccttcggctt ttcggaccag cggctcaagc aaatctacga gttcgagtac 23760gagggcttgc tgcgtcgcag cgccatcgcc tcctcgcccg accgctgcgt caccctcgaa 23820aagtccaccc agaccgtgca ggggcccgac tcggccgcct gcggtctctt ctgctgcatg 23880tttctgcacg cctttgtgca ctggcctcag agtcccatgg accgcaaccc caccatgaac 23940ttgctgacgg gggtgcccaa ctccatgctc cagagccccc aggtcgagcc caccctgcgc 24000cgcaaccagg agcagctcta cagcttcctg gagcgccact cgccttactt ccgccgccac 24060agcgcacaga tcaggagggc cacctccttc tgccacttgc aagagatgca agaagggtaa 24120taacgatgta cacacttttt ttctcaataa atggcatctt tttatttata caagctctct 24180ggggtattca tttcccacca ccacccgccg ttgtcgccat ctggctctat ttagaaatcg 24240aaagggttct gccgggagtc gccgtgcgcc acgggcaggg acacgttgcg atactggtag 24300cgggtgcccc acttgaactc gggcaccacc aggcgaggca gctcggggaa gttttcgctc 24360cacaggctgc gggtcagcac cagcgcgttc atcaggtcgg gcgccgagat cttgaagtcg 24420cagttggggc cgccgccctg cgcgcgcgag ttgcggtaca ccgggttgca gcactggaac 24480accaacagcg ccgggtgctt cacgctggcc agcacgctgc ggtcggagat cagctcggcg 24540tccaggtcct ccgcgttgct cagcgcgaac ggggtcatct tgggcacttg ccgccccagg 24600aagggcgcgt gccccggttt cgagttgcag tcgcagcgca gcgggatcag caggtgcccg 24660tgcccggact cggcgttggg gtacagcgcg cgcatgaagg cctgcatctg gcggaaggcc 24720atctgggcct tggcgccctc cgagaagaac atgccgcagg acttgcccga gaactggttt 24780gcggggcagc tggcgtcgtg caggcagcag cgcgcgtcgg tgttggcgat ctgcaccacg 24840ttgcgccccc accggttctt cacgatcttg gccttggacg attgctcctt cagcgcgcgc 24900tgcccgttct cgctggtcac atccatctcg atcacatgtt ccttgttcac catgctgctg 24960ccgtgcagac acttcagctc gccctccgtc tcggtgcagc ggtgctgcca cagcgcgcag 25020cccgtgggct cgaaagactt gtaggtcacc tccgcgaagg actgcaggta cccctgcaaa 25080aagcggccca tcatggtcac gaaggtcttg ttgctgctga aggtcagctg cagcccgcgg 25140tgctcctcgt tcagccaggt cttgcacacg gccgccagcg cctccacctg gtcgggcagc 25200atcttgaagt tcaccttcag ctcattctcc acgtggtact tgtccatcag cgtgcgcgcc 25260gcctccatgc ccttctccca ggccgacacc agcggcaggc tcacggggtt cttcaccatc 25320accgtggccg ccgcctccgc cgcgctttcg ctttccgccc cgctgttctc ttcctcttcc 25380tcctcttcct cgccgccgcc cactcgcagc ccccgcacca cggggtcgtc ttcctgcagg 25440cgctgcacct tgcgcttgcc gttgcgcccc tgcttgatgc gcacgggcgg gttgctgaag 25500cccaccatca ccagcgcggc ctcttcttgc tcgtcctcgc tgtccagaat gacctccggg 25560gagggggggt tggtcatcct cagtaccgag gcacgcttct ttttcttcct gggggcgttc 25620gccagctccg cggctgcggc cgctgccgag gtcgaaggcc gagggctggg cgtgcgcggc 25680accagcgcgt cctgcgagcc gtcctcgtcc tcctcggact cgagacggag gcgggcccgc 25740ttcttcgggg gcgcgcgggg cggcggaggc ggcggcggcg acggagacgg ggacgagaca 25800tcgtccaggg tgggtggacg gcgggccgcg ccgcgtccgc gctcgggggt ggtctcgcgc 25860tggtcctctt cccgactggc catctcccac tgctccttct cctataggca gaaagagatc 25920atggagtctc tcatgcgagt cgagaaggag gaggacagcc taaccgcccc ctctgagccc 25980tccaccaccg ccgccaccac cgccaatgcc gccgcggacg acgcgcccac cgagaccacc 26040gccagtacca ccctccccag cgacgcaccc ccgctcgaga atgaagtgct gatcgagcag 26100gacccgggtt ttgtgagcgg agaggaggat gaggtggatg agaaggagaa ggaggaggtc 26160gccgcctcag tgccaaaaga ggataaaaag caagaccagg acgacgcaga taaggatgag 26220acagcagtcg ggcgggggaa cggaagccat gatgctgatg acggctacct agacgtggga 26280gacgacgtgc tgcttaagca cctgcaccgc cagtgcgtca tcgtctgcga cgcgctgcag 26340gagcgctgcg aagtgcccct ggacgtggcg gaggtcagcc gcgcctacga gcggcacctc 26400ttcgcgccgc acgtgccccc caagcgccgg gagaacggca cctgcgagcc caacccgcgt 26460ctcaacttct acccggtctt cgcggtaccc gaggtgctgg ccacctacca catctttttc 26520caaaactgca agatccccct ctcctgccgc gccaaccgca cccgcgccga caaaaccctg 26580accctgcggc agggcgccca catacctgat atcgcctctc tggaggaagt gcccaagatc 26640ttcgagggtc tcggtcgcga cgagaaacgg gcggcgaacg ctctgcacgg agacagcgaa 26700aacgagagtc actcgggggt gctggtggag ctcgagggcg acaacgcgcg cctggccgta 26760ctcaagcgca gcatagaggt cacccacttt gcctacccgg cgctcaacct gccccccaag 26820gtcatgagtg tggtcatggg cgagctcatc atgcgccgcg cccagcccct ggccgcggat 26880gcaaacttgc aagagtcctc cgaggaaggc ctgcccgcgg tcagcgacga gcagctggcg 26940cgctggctgg agacccgcga ccccgcgcag ctggaggagc ggcgcaagct catgatggcc 27000gcggtgctgg tcaccgtgga gctcgagtgt ctgcagcgct tcttcgcgga ccccgagatg 27060cagcgcaagc tcgaggagac cctgcactac accttccgcc agggctacgt gcgccaggcc 27120tgcaagatct ccaacgtgga gctctgcaac ctggtctcct acctgggcat cctgcacgag 27180aaccgcctcg ggcagaacgt cctgcactcc accctcaaag gggaggcgcg ccgcgactac 27240atccgcgact gcgcctacct cttcctctgc tacacctggc agacggccat gggggtctgg 27300cagcagtgcc tggaggagcg caacctcaag gagctggaaa agctcctcaa gcgcaccctc 27360agggacctct ggacgggctt caacgagcgc tcggtggccg ccgcgctggc ggacatcatc 27420tttcccgagc gcctgctcaa gaccctgcag cagggcctgc ccgacttcac cagccagagc 27480atgctgcaga acttcaggac tttcatcctg gagcgctcgg gcatcctgcc ggccacttgc 27540tgcgcgctgc ccagcgactt cgtgcccatc aagtacaggg agtgcccgcc gccgctctgg 27600ggccactgct acctcttcca gctggccaac tacctcgcct accactcgga cctcatggaa 27660gacgtgagcg gcgagggcct gctcgagtgc cactgccgct gcaacctctg cacgccccac 27720cgctctctag tctgcaaccc gcagctgctc agcgagagtc agattatcgg taccttcgag 27780ctgcagggtc cctcgcctga cgagaagtcc gcggctccag ggctgaaact cactccgggg 27840ctgtggactt ccgcctacct acgcaaattt gtacctgagg actaccacgc ccacgagatc 27900aggttctacg aagaccaatc ccgcccgccc aaggcggagc tcaccgcctg cgtcatcacc 27960caggggcaca tcctgggcca attgcaagcc atcaacaaag cccgccgaga gttcttgctg 28020aaaaagggtc ggggggtgta cctggacccc cagtccggcg aggagctaaa cccgctaccc 28080ccgccgccgc cccagcagcg ggaccttgct tcccaggatg gcacccagaa agaagcagca 28140gccgccgccg ccgccgcagc catacatgct tctggaggaa gaggaggagg actgggacag 28200tcaggcagag gaggtttcgg acgaggagca ggaggagatg atggaagact gggaggagga 28260cagcagccta gacgaggaag cttcagaggc cgaagaggtg gcagacgcaa caccatcgcc 28320ctcggtcgca gccccctcgc cggggcccct gaaatcctcc gaacccagca ccagcgctat 28380aacctccgct cctccggcgc cggcgccacc cgcccgcaga cccaaccgta gatgggacac 28440cacaggaacc ggggtcggta agtccaagtg cccgccgccg ccaccgcagc agcagcagca 28500gcagcgccag ggctaccgct cgtggcgcgg gcacaagaac gccatagtcg cctgcttgca 28560agactgcggg ggcaacatct ctttcgcccg ccgcttcctg ctattccacc acggggtcgc 28620ctttccccgc aatgtcctgc attactaccg tcatctctac agcccctact gcagcggcga 28680cccagaggcg gcagcggcag ccacagcggc gaccaccacc taggaagata tcctccgcgg 28740gcaagacagc ggcagcagcg gccaggagac ccgcggcagc agcggcggga gcggtgggcg 28800cactgcgcct ctcgcccaac gaacccctct cgacccggga gctcagacac aggatcttcc 28860ccactttgta tgccatcttc caacagagca gaggccagga gcaggagctg aaaataaaaa 28920acagatctct gcgctccctc acccgcagct gtctgtatca caaaagcgaa gatcagcttc 28980ggcgcacgct ggaggacgcg gaggcactct tcagcaaata ctgcgcgctc actcttaaag 29040actagctccg cgcccttctc gaatttaggc gggagaaaac tacgtcatcg ccggccgccg 29100cccagcccgc ccagccgaga tgagcaaaga gattcccacg ccatacatgt ggagctacca 29160gccgcagatg ggactcgcgg cgggagcggc ccaggactac tccacccgca tgaactacat 29220gagcgcggga ccccacatga tctcacaggt caacgggatc cgcgcccagc gaaaccaaat 29280actgctggaa caggcggcca tcaccgccac gccccgccat aatctcaacc cccgaaattg 29340gcccgccgcc ctcgtgtacc aggaaacccc ctccgccacc accgtactac ttccgcgtga 29400cgcccaggcc gaagtccaga tgactaactc aggggcgcag ctcgcgggcg gctttcgtca 29460cggggcgcgg ccgctccgac caggtataag acacctgatg atcagaggcc gaggtatcca 29520gctcaacgac gagtcggtga gctcttcgct cggtctccgt ccggacggaa

ctttccagct 29580cgccggatcc ggccgctctt cgttcacgcc ccgccaggcg tacctgactc tgcagacctc 29640gtcctcggag ccccgctccg gcggcatcgg aaccctccag ttcgtggagg agttcgtgcc 29700ctcggtctac ttcaacccct tctcgggacc tcccggacgc taccccgacc agttcattcc 29760gaactttgac gcggtgaagg actcggcgga cggctacgac tgaatgtcag gtgtcgaggc 29820agagcagctt cgcctgagac acctcgagca ctgccgccgc cacaagtgct tcgcccgcgg 29880ttctggtgag ttctgctact ttcagctacc cgaggagcat accgaggggc cggcgcacgg 29940cgtccgcctg accacccagg gcgaggttac ctgttccctc atccgggagt ttaccctccg 30000tcccctgcta gtggagcggg agcggggtcc ctgtgtccta actatcgcct gcaactgccc 30060taaccctgga ttacatcaag atctttgctg tcatctctgt gctgagttta ataaacgctg 30120agatcagaat ctactggggc tcctgtcgcc atcctgtgaa cgccaccgtc ttcacccacc 30180ccgaccaggc ccaggcgaac ctcacctgcg gtctgcatcg gagggccaag aagtacctca 30240cctggtactt caacggcacc ccctttgtgg tttacaacag cttcgacggg gacggagtct 30300ccctgaaaga ccagctctcc ggtctcagct actccatcca caagaacacc accctccaac 30360tcttccctcc ctacctgccg ggaacctacg agtgcgtcac cggccgctgc acccacctca 30420cccgcctgat cgtaaaccag agctttccgg gaacagataa ctccctcttc cccagaacag 30480gaggtgagct caggaaactc cccggggacc agggcggaga cgtaccttcg acccttgtgg 30540ggttaggatt ttttattacc gggttgctgg ctcttttaat caaagtttcc ttgagatttg 30600ttctttcctt ctacgtgtat gaacacctca acctccaata actctaccct ttcttcggaa 30660tcaggtgact tctctgaaat cgggcttggt gtgctgctta ctctgttgat ttttttcctt 30720atcatactca gccttctgtg cctcaggctc gccgcctgct gcgcacacat ctatatctac 30780tgctggttgc tcaagtgcag gggtcgccac ccaagatgaa caggtacatg gtcctatcga 30840tcctaggcct gctggccctg gcggcctgca gcgccgccaa aaaagagatt acctttgagg 30900agcccgcttg caatgtaact ttcaagcccg agggtgacca atgcaccacc ctcgtcaaat 30960gcgttaccaa tcatgagagg ctgcgcatcg actacaaaaa caaaactggc cagtttgcgg 31020tctatagtgt gtttacgccc ggagacccct ctaactactc tgtcaccgtc ttccagggcg 31080gacagtctaa gatattcaat tacactttcc ctttttatga gttatgcgat gcggtcatgt 31140acatgtcaaa acagtacaac ctgtggcctc cctctcccca ggcgtgtgtg gaaaatactg 31200ggtcttactg ctgtatggct ttcgcaatca ctacgctcgc tctaatctgc acggtgctat 31260acataaaatt caggcagagg cgaatcttta tcgatgaaaa gaaaatgcct tgatcgctaa 31320caccggcttt ctatctgcag aatgaatgca atcacctccc tactaatcac caccaccctc 31380cttgcgattg cccatgggtt gacacgaatc gaagtgccag tggggtccaa tgtcaccatg 31440gtgggccccg ccggcaattc caccctcatg tgggaaaaat ttgtccgcaa tcaatgggtt 31500catttctgct ctaaccgaat cagtatcaag cccagagcca tctgcgatgg gcaaaatcta 31560actctgatca atgtgcaaat gatggatgct gggtactatt acgggcagcg gggagaaatc 31620attaattact ggcgacccca caaggactac atgctgcatg tagtcgaggc acttcccact 31680accaccccca ctaccacctc tcccaccacc accaccacta ctactactac tactactact 31740actactacta ccactaccgc tgcccgccat acccgcaaaa gcaccatgat tagcacaaag 31800ccccctcgtg ctcactccca cgccggcggg cccatcggtg cgacctcaga aaccaccgag 31860ctttgcttct gccaatgcac taacgccagc gctcatgaac tgttcgacct ggagaatgag 31920gatgtccagc agagctccgc ttgcctgacc caggaggctg tggagcccgt tgccctgaag 31980cagatcggtg attcaataat tgactcttct tcttttgcca ctcccgaata ccctcccgat 32040tctactttcc acatcacggg taccaaagac cctaacctct ctttctacct gatgctgctg 32100ctctgtatct ctgtggtctc ttccgcgctg atgttactgg ggatgttctg ctgcctgatc 32160tgccgcagaa agagaaaagc tcgctctcag ggccaaccac tgatgccctt cccctacccc 32220ccggattttg cagataacaa gatatgagct cgctgctgac actaaccgct ttactagcct 32280gcgctctaac ccttgtcgct tgcgactcga gattccacaa tgtcacagct gtggcaggag 32340aaaatgttac tttcaactcc acggccgata cccagtggtc gtggagtggc tcaggtagct 32400acttaactat ctgcaatagc tccacttccc ccggcatatc cccaaccaag taccaatgca 32460atgccagcct gttcaccctc atcaacgctt ccaccctgga caatggactc tatgtaggct 32520atgtaccctt tggtgggcaa ggaaagaccc acgcttacaa cctggaagtt cgccagccca 32580gaaccactac ccaagcttct cccaccacca ccaccaccac caccatcacc agcagcagca 32640gcagcagcag ccacagcagc agcagcagat tattgacttt ggttttggcc agctcatctg 32700ccgctaccca ggccatctac agctctgtgc ccgaaaccac tcagatccac cgcccagaaa 32760cgaccaccgc caccacccta cacacctcca gcgatcagat gccgaccaac atcaccccct 32820tggctcttca aatgggactt acaagcccca ctccaaaacc agtggatgcg gccgaggtct 32880ccgccctcgt caatgactgg gcggggctgg gaatgtggtg gttcgccata ggcatgatgg 32940cgctctgcct gcttctgctc tggctcatct gctgcctcca ccgcaggcga gccagacccc 33000ccatctatag acccatcatt gtcctgaacc ccgataatga tgggatccat agattggatg 33060gcctgaaaaa cctacttttt tcttttacag tatgataaat tgagacatgc ctcgcatttt 33120cttgtacatg ttccttctcc caccttttct ggggtgttct acgctggccg ctgtgtctca 33180cctggaggta gactgcctct cacccttcac tgtctacctg ctttacggat tggtcaccct 33240cactctcatc tgcagcctaa tcacagtaat catcgccttc atccagtgca ttgattacat 33300ctgtgtgcgc ctcgcatact tcagacacca cccgcagtac cgagacagga acattgccca 33360acttctaaga ctgctctaat catgcataag actgtgatct gccttctgat cctctgcatc 33420ctgcccaccc tcacctcctg ccagtacacc acaaaatctc cgcgcaaaag acatgcctcc 33480tgccgcttca cccaactgtg gaatataccc aaatgctaca acgaaaagag cgagctctcc 33540gaagcttggc tgtatggggt catctgtgtc ttagttttct gcagcactgt ctttgccctc 33600ataatctacc cctactttga tttgggatgg aacgcgatcg atgccatgaa ttaccccacc 33660tttcccgcac ccgagataat tccactgcga caagttgtac ccgttgtcgt taatcaacgc 33720cccccatccc ctacgcccac tgaaatcagc tactttaacc taacaggcgg agatgactga 33780cgccctagat ctagaaatgg acggcatcag taccgagcag cgtctcctag agaggcgcag 33840gcaggcggct gagcaagagc gcctcaatca ggagctccga gatctcgtta acctgcacca 33900gtgcaaaaga ggcatctttt gtctggtaaa gcaggccaaa gtcacctacg agaagaccgg 33960caacagccac cgcctcagtt acaaattgcc cacccagcgc cagaagctgg tgctcatggt 34020gggtgagaat cccatcaccg tcacccagca ctcggtagag accgaggggt gtctgcactc 34080cccctgtcgg ggtccagaag acctctgcac cctggtaaag accctgtgcg gtctcagaga 34140tttagtcccc tttaactaat caaacactgg aatcaataaa aagaatcact tacttaaaat 34200cagacagcag gtctctgtcc agtttattca gcagcacctc cttcccctcc tcccaactct 34260ggtactccaa acgccttctg gcggcaaact tcctccacac cctgaaggga atgtcagatt 34320cttgctcctg tccctccgca cccactatct tcatgttgtt gcagatgaag cgcaccaaaa 34380cgtctgacga gagcttcaac cccgtgtacc cctatgacac ggaaagcggc cctccctccg 34440tccctttcct cacccctccc ttcgtgtctc ccgatggatt ccaagaaagt ccccccgggg 34500tcctgtctct gaacctggcc gagcccctgg tcacttccca cggcatgctc gccctgaaaa 34560tgggaagtgg cctctccctg gacgacgctg gcaacctcac ctctcaagat atcaccaccg 34620ctagccctcc cctcaaaaaa accaagacca acctcagcct agaaacctca tcccccctaa 34680ctgtgagcac ctcaggcgcc ctcaccgtag cagccgccgc tcccctggcg gtggccggca 34740cctccctcac catgcaatca gaggcccccc tgacagtaca ggatgcaaaa ctcaccctgg 34800ccaccaaagg ccccctgacc gtgtctgaag gcaaactggc cttgcaaaca tcggccccgc 34860tgacggccgc tgacagcagc accctcacag tcagtgccac accacccctt agcacaagca 34920atggcagctt gggtattgac atgcaagccc ccatttacac caccaatgga aaactaggac 34980ttaactttgg cgctcccctg catgtggtag acagcctaaa tgcactgact gtagttactg 35040gccaaggtct tacgataaac ggaacagccc tacaaactag agtctcaggt gccctcaact 35100atgacacatc aggaaaccta gaattgagag ctgcaggggg tatgcgagtt gatgcaaatg 35160gtcaacttat ccttgatgta gcttacccat ttgatgcaca aaacaatctc agccttaggc 35220ttggacaggg acccctgttt gttaactctg cccacaactt ggatgttaac tacaacagag 35280gcctctacct gttcacatct ggaaatacca aaaagctaga agttaatatc aaaacagcca 35340agggtctcat ttatgatgac actgctatag caatcaatgc gggtgatggg ctacagtttg 35400actcaggctc agatacaaat ccattaaaaa ctaaacttgg attaggactg gattatgact 35460ccagcagagc cataattgct aaactgggaa ctggcctaag ctttgacaac acaggtgcca 35520tcacagtagg caacaaaaat gatgacaagc ttaccttgtg gaccacacca gacccatccc 35580ctaactgtag aatctattca gagaaagatg ctaaattcac acttgttttg actaaatgcg 35640gcagtcaggt gttggccagc gtttctgttt tatctgtaaa aggtagcctt gcgcccatca 35700gtggcacagt aactagtgct cagattgtcc tcagatttga tgaaaatgga gttctactaa 35760gcaattcttc ccttgaccct caatactgga actacagaaa aggtgacctt acagagggca 35820ctgcatatac caacgcagtg ggatttatgc ccaacctcac agcataccca aaaacacaga 35880gccaaactgc taaaagcaac attgtaagtc aggtttactt gaatggggac aaatccaaac 35940ccatgaccct caccattacc ctcaatggaa ctaatgaaac aggagatgcc acagtaagca 36000cttactccat gtcattctca tggaactgga atggaagtaa ttacattaat gaaacgttcc 36060aaaccaactc cttcaccttc tcctacatcg cccaagaata aaaagcatga cgctgttgat 36120ttgattcaat gtgtttctgt tttattttca agcacaacaa aatcattcaa gtcattcttc 36180catcttagct taatagacac agtagcttaa tagacccagt agtgcaaagc cccattctag 36240cttataacta gtggagaagt actcgcctac atgggggtag agtcataatc gtgcatcagg 36300atagggcggt ggtgctgcag cagcgcgcga ataaactgct gccgccgccg ctccgtcctg 36360caggaataca acatggcagt ggtctcctca gcgatgattc gcaccgcccg cagcataagg 36420cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac ttaaatcagc acagtaactg 36480cagcacagca ccacaatatt gttcaaaatc ccacagtgca aggcgctgta tccaaagctc 36540atggcgggga ccacagaacc cacgtggcca tcataccaca agcgcaggta gattaagtgg 36600cgacccctca taaacacgct ggacataaac attacctctt ttggcatgtt gtaattcacc 36660acctcccggt accatataaa cctctgatta aacatggcgc catccaccac catcctaaac 36720cagctggcca aaacctgccc gccggctata cactgcaggg aaccgggact ggaacaatga 36780cagtggagag cccaggactc gtaaccatgg atcatcatgc tcgtcatgat atcaatgttg 36840gcacaacaca ggcacacgtg catacacttc ctcaggatta caagctcctc ccgcgttaga 36900accatatccc agggaacaac ccattcctga atcagcgtaa atcccacact gcagggaaga 36960cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac attcgggcag cagcggatga 37020tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag gtagacgatc cctactgtac 37080ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg tcatgccaaa tggaacgccg 37140gacgtagtca tatttcctga agtcttagat ctctcaacgc agcaccagca ccaacacttc 37200gcagtgtaaa aggccaagtg ccgagagagt atatatagga ataaaaagtg acgtaaacgg 37260gcaaagtcca aaaaacgccc agaaaaaccg cacgcgaacc tacgccccga aacgaaagcc 37320aaaaaacact agacactccc ttccggcgtc aacttccgct ttcccacgct acgtcacttg 37380ccccagtcaa acaaactaca tatcccgaac ttccaagtcg ccacgcccaa aacaccgcct 37440acacctcccc gcccgccggc ccgcccccaa acccgcctcc cgccccgcgc cccgccccgc 37500gccgcccatc tcattatcat attggcttca atccaaaata aggtatatta ttgatgatg 3755951145PRTArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polypeptide" 5Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr1 5 10 15Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 20 25 30Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 35 40 45Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 50 55 60Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys65 70 75 80Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 85 90 95Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 100 105 110Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 115 120 125Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 130 135 140Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser Lys Val Leu His Leu145 150 155 160Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 165 170 175Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 180 185 190Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 195 200 205Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 210 215 220Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn225 230 235 240Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 245 250 255Leu Leu Ser Leu Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys Leu 260 265 270Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile Met 275 280 285Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro Leu 290 295 300Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro Leu305 310 315 320Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg Thr 325 330 335Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe Pro 340 345 350Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp Thr 355 360 365Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val Asp 370 375 380Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr Asp385 390 395 400Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys Tyr 405 410 415Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile Lys 420 425 430Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp Thr 435 440 445Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly Lys 450 455 460Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro Leu465 470 475 480Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn Glu 485 490 495Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu Leu 500 505 510His Asn Val Asn Ala Gly Lys Ser Thr Thr Asn Arg Lys Arg Arg Ala 515 520 525Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp 530 535 540Val Glu Ser Asn Pro Gly Pro Met Ala Leu Ser Lys Val Lys Leu Asn545 550 555 560Asp Thr Leu Asn Lys Asp Gln Leu Leu Ser Ser Ser Lys Tyr Thr Ile 565 570 575Gln Arg Ser Thr Gly Asp Ser Ile Asp Thr Pro Asn Tyr Asp Val Gln 580 585 590Lys His Ile Asn Lys Leu Cys Gly Met Leu Leu Ile Thr Glu Asp Ala 595 600 605Asn His Lys Phe Thr Gly Leu Ile Gly Met Leu Tyr Ala Met Ser Arg 610 615 620Leu Gly Arg Glu Asp Thr Ile Lys Ile Leu Arg Asp Ala Gly Tyr His625 630 635 640Val Lys Ala Asn Gly Val Asp Val Thr Thr His Arg Gln Asp Ile Asn 645 650 655Gly Lys Glu Met Lys Phe Glu Val Leu Thr Leu Ala Ser Leu Thr Thr 660 665 670Glu Ile Gln Ile Asn Ile Glu Ile Glu Ser Arg Lys Ser Tyr Lys Lys 675 680 685Met Leu Lys Glu Met Gly Glu Val Ala Pro Glu Tyr Arg His Asp Ser 690 695 700Pro Asp Cys Gly Met Ile Ile Leu Cys Ile Ala Ala Leu Val Ile Thr705 710 715 720Lys Leu Ala Ala Gly Asp Arg Ser Gly Leu Thr Ala Val Ile Arg Arg 725 730 735Ala Asn Asn Val Leu Lys Asn Glu Met Lys Arg Tyr Lys Gly Leu Leu 740 745 750Pro Lys Asp Ile Ala Asn Ser Phe Tyr Glu Val Phe Glu Lys Tyr Pro 755 760 765His Phe Ile Asp Val Phe Val His Phe Gly Ile Ala Gln Ser Ser Thr 770 775 780Arg Gly Gly Ser Arg Val Glu Gly Ile Phe Ala Gly Leu Phe Met Asn785 790 795 800Ala Tyr Gly Ala Gly Gln Val Met Leu Arg Trp Gly Val Leu Ala Lys 805 810 815Ser Val Lys Asn Ile Met Leu Gly His Ala Ser Val Gln Ala Glu Met 820 825 830Glu Gln Val Val Glu Val Tyr Glu Tyr Ala Gln Lys Leu Gly Gly Glu 835 840 845Ala Gly Phe Tyr His Ile Leu Asn Asn Pro Lys Ala Ser Leu Leu Ser 850 855 860Leu Thr Gln Phe Pro His Phe Ser Ser Val Val Leu Gly Asn Ala Ala865 870 875 880Gly Leu Gly Ile Met Gly Glu Tyr Arg Gly Thr Pro Arg Asn Gln Asp 885 890 895Leu Tyr Asp Ala Ala Lys Ala Tyr Ala Glu Gln Leu Lys Glu Asn Gly 900 905 910Val Ile Asn Tyr Ser Val Leu Asp Leu Thr Ala Glu Glu Leu Glu Ala 915 920 925Ile Lys His Gln Leu Asn Pro Lys Asp Asn Asp Val Glu Leu Gly Gly 930 935 940Gly Gly Ser Gly Gly Gly Gly Met Ser Arg Arg Asn Pro Cys Lys Phe945 950 955 960Glu Ile Arg Gly His Cys Leu Asn Gly Lys Arg Cys His Phe Ser His 965 970 975Asn Tyr Phe Glu Trp Pro Pro His Ala Leu Leu Val Arg Gln Asn Phe 980 985 990Met Leu Asn Arg Ile Leu Lys Ser Met Asp Lys Ser Ile Asp Thr Leu 995 1000 1005Ser Glu Ile Ser Gly Ala Ala Glu Leu Asp Arg Thr Glu Glu Tyr 1010 1015 1020Ala Leu Gly Val Val Gly Val Leu Glu Ser Tyr Ile Gly Ser Ile 1025 1030 1035Asn Asn Ile Thr Lys Gln Ser Ala Cys Val Ala Met Ser Lys Leu 1040 1045 1050Leu Thr Glu Leu Asn Ser Asp Asp Ile Lys Lys Leu Arg Asp Asn 1055 1060 1065Glu Glu Leu Asn Ser Pro Lys Ile Arg Val Tyr Asn Thr Val Ile 1070 1075 1080Ser Tyr Ile Glu Ser Asn Arg Lys Asn Asn Lys Gln Thr Ile His 1085 1090 1095Leu Leu Lys Arg Leu Pro Ala Asp Val Leu Lys Lys Thr Ile Lys 1100 1105 1110Asn Thr Leu Asp Ile His Lys Ser Ile Thr Ile Asn Asn Pro Lys 1115 1120 1125Glu Ser Thr Val Ser Asp Thr Asn Asp His Ala Lys Asn Asn Asp 1130 1135 1140Thr

Thr 114561187DNAArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polynucleotide" 6ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca 60ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca 120ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 180ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 240acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 300ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 360aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 420tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 480gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 540gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 600ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag gcgaagcgct 660ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgcggcgg 720gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc 780ccgccccggc tctgactgac cgcgttacta aaacaggtaa gtccggcctc cgcgccgggt 840tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg 900gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata 960agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga 1020ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct 1080cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgatgcc tctactaacc 1140atgttcatgt tttctttttt tttctacagg tcctgggtga cgaacag 118772908DNAArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polynucleotide" 7ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca 60ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca 120ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 180ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 240acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 300ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 360aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 420tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 480gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 540gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 600ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc 660cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg 720aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg 780gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag 840agtgagatct tccgtttatc taggtaccag atatcgccac catggccctg agcaaagtga 900aactgaacga tacactgaac aaggaccagc tgctgtccag cagcaagtac accatccagc 960ggagcaccgg cgacagcatc gataccccca actacgacgt gcagaagcac atcaacaagc 1020tgtgcggcat gctgctgatc acagaggacg ccaaccacaa gttcaccggc ctgatcggca 1080tgctgtacgc catgagccgg ctgggccggg aggacaccat caagatcctg cgggacgccg 1140gctaccacgt gaaggccaat ggcgtggacg tgaccacaca ccggcaggac atcaacggca 1200aagaaatgaa gttcgaggtg ctgaccctgg ccagcctgac caccgagatc cagatcaata 1260tcgagatcga gagccggaag tcctacaaga aaatgctgaa agaaatgggc gaggtggccc 1320ccgagtacag acacgacagc cccgactgcg gcatgatcat cctgtgtatc gccgccctgg 1380tgatcacaaa gctggccgct ggcgacagat ctggcctgac agccgtgatc agacgggcca 1440acaatgtgct gaagaacgag atgaagcggt acaagggcct gctgcccaag gacattgcca 1500acagcttcta cgaggtgttc gagaagtacc cccacttcat cgacgtgttc gtgcacttcg 1560gcattgccca gagcagcacc agaggcggct ccagagtgga gggcatcttc gccggcctgt 1620tcatgaacgc ctacggcgct ggccaggtga tgctgagatg gggcgtgctg gccaagagcg 1680tgaagaacat catgctgggc cacgccagcg tgcaggccga gatggaacag gtggtggagg 1740tgtacgagta cgcccagaag ctgggcggag aggccggctt ctaccacatc ctgaacaacc 1800ctaaggcctc cctgctgtcc ctgacccagt tcccccactt ctccagcgtg gtgctgggaa 1860atgccgccgg actgggcatc atgggcgagt accggggcac ccccagaaac caggacctgt 1920acgacgccgc caaggcctac gccgagcagc tgaaagaaaa cggcgtgatc aactacagcg 1980tgctggacct gaccgctgag gaactggaag ccatcaagca ccagctgaac cccaaggaca 2040acgacgtgga gctgggaggc ggaggatctg gcggcggagg catgagcaga cggaacccct 2100gcaagttcga gatccggggc cactgcctga acggcaagcg gtgccacttc agccacaact 2160acttcgagtg gccccctcat gctctgctgg tgcggcagaa cttcatgctg aaccggatcc 2220tgaagtccat ggacaagagc atcgacaccc tgagcgagat cagcggagcc gccgagctgg 2280acagaaccga ggaatatgcc ctgggcgtgg tgggagtgct ggaaagctac atcggctcca 2340tcaacaacat cacaaagcag agcgcctgcg tggccatgag caagctgctg acagagctga 2400acagcgacga catcaagaag ctgagggaca acgaggaact gaacagcccc aagatccggg 2460tgtacaacac cgtgatcagc tacattgaga gcaaccgcaa gaacaacaag cagaccatcc 2520atctgctgaa gcggctgccc gccgacgtgc tgaaaaagac catcaagaac accctggaca 2580tccacaagtc catcaccatc aacaatccca aagaaagcac cgtgtctgac accaacgatc 2640acgccaagaa caacgacacc acctgatgag cggccgcgat ctgctgtgcc ttctagttgc 2700cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 2760actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 2820attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 2880catgctgggg atgcggtggg ctctatgg 29088594PRTArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polypeptide" 8Met Ala Leu Ser Lys Val Lys Leu Asn Asp Thr Leu Asn Lys Asp Gln1 5 10 15Leu Leu Ser Ser Ser Lys Tyr Thr Ile Gln Arg Ser Thr Gly Asp Ser 20 25 30Ile Asp Thr Pro Asn Tyr Asp Val Gln Lys His Ile Asn Lys Leu Cys 35 40 45Gly Met Leu Leu Ile Thr Glu Asp Ala Asn His Lys Phe Thr Gly Leu 50 55 60Ile Gly Met Leu Tyr Ala Met Ser Arg Leu Gly Arg Glu Asp Thr Ile65 70 75 80Lys Ile Leu Arg Asp Ala Gly Tyr His Val Lys Ala Asn Gly Val Asp 85 90 95Val Thr Thr His Arg Gln Asp Ile Asn Gly Lys Glu Met Lys Phe Glu 100 105 110Val Leu Thr Leu Ala Ser Leu Thr Thr Glu Ile Gln Ile Asn Ile Glu 115 120 125Ile Glu Ser Arg Lys Ser Tyr Lys Lys Met Leu Lys Glu Met Gly Glu 130 135 140Val Ala Pro Glu Tyr Arg His Asp Ser Pro Asp Cys Gly Met Ile Ile145 150 155 160Leu Cys Ile Ala Ala Leu Val Ile Thr Lys Leu Ala Ala Gly Asp Arg 165 170 175Ser Gly Leu Thr Ala Val Ile Arg Arg Ala Asn Asn Val Leu Lys Asn 180 185 190Glu Met Lys Arg Tyr Lys Gly Leu Leu Pro Lys Asp Ile Ala Asn Ser 195 200 205Phe Tyr Glu Val Phe Glu Lys Tyr Pro His Phe Ile Asp Val Phe Val 210 215 220His Phe Gly Ile Ala Gln Ser Ser Thr Arg Gly Gly Ser Arg Val Glu225 230 235 240Gly Ile Phe Ala Gly Leu Phe Met Asn Ala Tyr Gly Ala Gly Gln Val 245 250 255Met Leu Arg Trp Gly Val Leu Ala Lys Ser Val Lys Asn Ile Met Leu 260 265 270Gly His Ala Ser Val Gln Ala Glu Met Glu Gln Val Val Glu Val Tyr 275 280 285Glu Tyr Ala Gln Lys Leu Gly Gly Glu Ala Gly Phe Tyr His Ile Leu 290 295 300Asn Asn Pro Lys Ala Ser Leu Leu Ser Leu Thr Gln Phe Pro His Phe305 310 315 320Ser Ser Val Val Leu Gly Asn Ala Ala Gly Leu Gly Ile Met Gly Glu 325 330 335Tyr Arg Gly Thr Pro Arg Asn Gln Asp Leu Tyr Asp Ala Ala Lys Ala 340 345 350Tyr Ala Glu Gln Leu Lys Glu Asn Gly Val Ile Asn Tyr Ser Val Leu 355 360 365Asp Leu Thr Ala Glu Glu Leu Glu Ala Ile Lys His Gln Leu Asn Pro 370 375 380Lys Asp Asn Asp Val Glu Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly385 390 395 400Met Ser Arg Arg Asn Pro Cys Lys Phe Glu Ile Arg Gly His Cys Leu 405 410 415Asn Gly Lys Arg Cys His Phe Ser His Asn Tyr Phe Glu Trp Pro Pro 420 425 430His Ala Leu Leu Val Arg Gln Asn Phe Met Leu Asn Arg Ile Leu Lys 435 440 445Ser Met Asp Lys Ser Ile Asp Thr Leu Ser Glu Ile Ser Gly Ala Ala 450 455 460Glu Leu Asp Arg Thr Glu Glu Tyr Ala Leu Gly Val Val Gly Val Leu465 470 475 480Glu Ser Tyr Ile Gly Ser Ile Asn Asn Ile Thr Lys Gln Ser Ala Cys 485 490 495Val Ala Met Ser Lys Leu Leu Thr Glu Leu Asn Ser Asp Asp Ile Lys 500 505 510Lys Leu Arg Asp Asn Glu Glu Leu Asn Ser Pro Lys Ile Arg Val Tyr 515 520 525Asn Thr Val Ile Ser Tyr Ile Glu Ser Asn Arg Lys Asn Asn Lys Gln 530 535 540Thr Ile His Leu Leu Lys Arg Leu Pro Ala Asp Val Leu Lys Lys Thr545 550 555 560Ile Lys Asn Thr Leu Asp Ile His Lys Ser Ile Thr Ile Asn Asn Pro 565 570 575Lys Glu Ser Thr Val Ser Asp Thr Asn Asp His Ala Lys Asn Asn Asp 580 585 590Thr Thr93617DNAArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polynucleotide" 9ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca 60ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca 120ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 180ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 240acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 300ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 360aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 420tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 480gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 540gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 600ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag gcgaagcgct 660ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgcggcgg 720gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc 780ccgccccggc tctgactgac cgcgttacta aaacaggtaa gtccggcctc cgcgccgggt 840tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg 900gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata 960agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga 1020ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct 1080cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgatgcc tctactaacc 1140atgttcatgt tttctttttt tttctacagg tcctgggtga cgaacaggat atcgccacca 1200tggaactgct gatcctgaag gccaacgcca tcaccaccat cctgaccgcc gtgaccttct 1260gcttcgccag cggccagaac atcaccgagg aattctacca gagcacctgt agcgccgtga 1320gcaagggcta cctgagcgcc ctgagaaccg gctggtacac cagcgtgatc accatcgagc 1380tgagcaacat caaagaaaac aagtgcaacg gcaccgacgc caaagtgaag ctgatcaagc 1440aggaactgga caagtacaag aacgccgtga ccgagctgca gctgctgatg cagagcaccc 1500ccgccaccaa caaccgggcc agacgggagc tgccccggtt catgaactac accctgaaca 1560acgccaaaaa gaccaacgtg accctgagca agaagcggaa gcggcggttc ctgggctttc 1620tgctgggcgt gggcagcgcc attgccagcg gcgtggccgt gtctaaggtg ctgcacctgg 1680aaggcgaagt gaacaagatc aagagcgccc tgctgagcac caacaaggcc gtggtgtccc 1740tgagcaacgg cgtgagcgtg ctgaccagca aggtgctgga tctgaagaac tacatcgaca 1800agcagctgct gcccatcgtg aacaagcaga gctgcagcat cagcaacatc gagacagtga 1860tcgagttcca gcagaagaac aaccggctgc tggaaatcac ccgggagttc agcgtgaacg 1920ccggcgtgac cacccctgtg tccacctaca tgctgaccaa cagcgagctg ctgagcctga 1980tcaacgacat gcccatcacc aacgaccaga aaaagctgat gagcaacaac gtgcagatcg 2040tgcggcagca gagctactcc atcatgtcca tcatcaaaga agaggtgctg gcctacgtgg 2100tgcagctgcc cctgtacggc gtgatcgaca ccccctgctg gaagctgcac accagccccc 2160tgtgcaccac caacaccaaa gagggcagca acatctgcct gacccggacc gacagaggct 2220ggtactgcga caacgccggc agcgtgtcat tctttccaca ggccgagaca tgcaaggtgc 2280agagcaaccg ggtgttctgc gacaccatga acagcctgac cctgccctcc gaagtgaacc 2340tgtgcaacgt ggacatcttc aaccccaagt acgactgcaa gatcatgacc tccaagaccg 2400acgtgtccag ctccgtgatc acctccctgg gcgccatcgt gtcctgctac ggcaagacca 2460agtgcaccgc cagcaacaag aaccggggca tcatcaagac cttcagcaac ggctgcgact 2520acgtgtccaa caagggggtg gacaccgtgt ccgtgggcaa caccctgtac tacgtgaaca 2580aacaggaagg caagagcctg tacgtgaagg gcgagcccat catcaacttc tacgaccccc 2640tggtgttccc cagcgacgag ttcgacgcca gcatcagcca ggtgaacgag aagatcaacc 2700agagcctggc cttcatccgg aagtccgacg agctgctgca caatgtgaat gccggcaagt 2760ccaccaccaa ctgatgagcg gccatctaat caacctctgg attacaaaat ttgtgaaaga 2820ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 2880cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 2940tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 3000actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 3060tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 3120gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 3180aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 3240tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 3300ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 3360tgggccgcct ccccgcctgc ggccgcgatc tgctgtgcct tctagttgcc agccatctgt 3420tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc 3480ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg 3540tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga 3600tgcggtgggc tctatgg 361710524PRTArtificial Sequencesource/note="Description of Artificial Sequence Synthetic polypeptide" 10Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr1 5 10 15Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 20 25 30Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 35 40 45Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 50 55 60Lys Glu Asn Lys Cys Asn Gly Thr Asp Ala Lys Val Lys Leu Ile Lys65 70 75 80Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 85 90 95Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 100 105 110Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 115 120 125Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 130 135 140Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser Lys Val Leu His Leu145 150 155 160Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 165 170 175Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 180 185 190Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 195 200 205Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 210 215 220Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn225 230 235 240Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 245 250 255Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 260 265 270Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 275 280 285Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 290 295 300Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro305 310 315 320Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 325 330 335Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 340 345 350Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 355 360 365Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 370 375 380Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr385 390 395 400Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 405 410 415Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 420 425 430Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 435 440 445Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 450 455 460Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro465 470 475 480Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 485 490 495Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 500 505 510Leu His Asn Val Asn Ala Gly Lys Ser Thr Thr Asn

515 520117PRTArtificial Sequencesource/note="Description of Artificial Sequence Synthetic peptide" 11Gly Gly Gly Ser Gly Gly Gly1 5129PRTArtificial Sequencesource/note="Description of Artificial Sequence Synthetic peptide" 12Gly Gly Gly Gly Ser Gly Gly Gly Gly1 5

Claims

1-22. (canceled)

23. A simian adenoviral vector comprising two expression cassettes, wherein each expression cassette comprises a transgene and a promoter, wherein the first expression cassette is inserted in the E1 region of the simian adenoviral vector, and the second expression cassette is inserted in a region of the adenoviral vector that is compatible with vector replication.

24. The simian adenoviral vector of claim 23, wherein the second expression cassette is inserted in the E3 region, between the stop codons of L5 and E4 (the "HE1" region) or between the end of the ITR and the cap site of E4 mRNA (the "HE2" region) of the simian adenoviral vector.

25. The simian adenoviral vector of claim 24, wherein the second expression cassette is inserted in the E3 region of the simian adenoviral vector.

26. The simian adenoviral vector of claim 24, wherein the second expression cassette is inserted in the HE1 region of the simian adenoviral vector.

27. The simian adenoviral vector of claim 24, wherein the second expression cassette is inserted in the HE2 region of the simian adenoviral vector.

28. The simian adenoviral vector of claim 23, wherein the vector is a chimpanzee adenoviral vector.

29. The simian adenoviral vector of claim 23, wherein the vector is an adenovirus.

30. The simian adenoviral vector of claim 29, wherein the adenovirus is ChAd155.

31. The simian adenoviral vector of claim 29, wherein the adenovirus is ChAd83.

32. The simian adenoviral vector of claim 23, wherein the first expression cassette and/or the second expression cassette comprises a human CMV or an enhanced human CMV promoter.

33. The simian adenoviral vector of claim 32, wherein the enhanced hCMV promoter has a nucleic acid sequence having at least about 90% sequence identity to SEQ ID NO: 6.

34. The simian adenoviral vector of claim 33, wherein the enhanced hCMV promoter has a nucleic acid sequence having at least about 96% sequence identity to SEQ ID NO: 6.

35. The simian adenoviral vector of claim 32, wherein the promoter comprises the nucleic acid sequence of SEQ ID NO: 6.

36. The simian adenoviral vector of claim 23, wherein the first and second expression cassettes comprise different promoters.

37. The simian adenoviral vector of claim 23, wherein the adenoviral vector is capable of infecting a mammalian cell.

38. The simian adenoviral vector of claim 23, wherein the first and/or the second expression cassette further comprises a posttranscriptional regulatory element.

39. The simian adenoviral vector of claim 38, wherein the posttranscriptional regulatory element is a Woodchuck Hepatitis Postranscriptional Regulatory Element.

40. A composition comprising the simian adenoviral vector of claim 23 and a pharmaceutically acceptable excipient.

41. A method of inducing an immune response against a disease caused by a pathogen in a subject in need thereof comprising administering an immunologically effective amount of the simian adenoviral vector of claim 23 to the subject.

42. The method of claim 41, wherein the simian adenoviral vector is a vaccine component.