TRANSGENIC PLANTS WITH ENHANCED TRAITS

Abstract

This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency, and enhanced drought tolerance or water use efficiency. Transgenic plants may include field crops as well as plant propagules and progeny of such transgenic plants. Methods of making and using such transgenic plants are also provided. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed, and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

Description

INCORPORATION OF SEQUENCE LISTING

[0001] The sequence listing file named "61673US0000_ST25.txt", which is 323 kilobytes (measured in MS-WINDOWS) and was created on Mar. 18, 2016, is filed herewith and incorporated herein by reference in its entirety.

FIELD OF THE INVENTION

[0002] Disclosed herein are recombinant DNA constructs, plants having altered phenotypes, enhanced traits, increased yield, increased nitrogen use efficiency and increased water use efficiency; propagules, progenies and field crops of such plants; and methods of making and using such plants. Also disclosed are methods of producing seed from such plants, growing such seed and/or selecting progeny plants with altered phenotypes, enhanced traits, increased yield, increased nitrogen use efficiency and increased water use efficiency.

SUMMARY

[0003] In one aspect, the present disclosure provides recombinant DNA constructs each comprising: a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29; or a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92. The recombinant DNA construct may comprise a promoter, such as a heterologous promoter, functional in a plant cell and operably linked to the polynucleotide sequence. Vectors, plasmids, plants, propagules and plant cells are further provided comprising such a recombinant DNA construct.

[0004] Plants comprising a recombinant DNA construct may be a field crop plant, such as corn, soybean, cotton, canola, rice, barley, oat, wheat, turf grass, alfalfa, sugar beet, sunflower, quinoa and sugarcane. A plant comprising a recombinant DNA construct may have an altered phenotype or an enhanced trait as compared to a control plant. The enhanced trait may be, for example, decreased days from planting to maturity, increased stalk size, increased number of leaves, increased plant height growth rate in vegetative stage, increased ear size, increased ear dry weight per plant, increased number of kernels per ear, increased weight per kernel, increased number of kernels per plant, decreased ear void, extended grain fill period, reduced plant height, increased number of root branches, increased total root length, increased yield, increased nitrogen use efficiency, and increased water use efficiency as compared to a control plant. The altered phenotype may be, for example, plant height, biomass, canopy area, anthocyanin content, chlorophyll content, water applied, water content, and water use efficiency.

[0005] According to another aspect, the present disclosure provides methods for altering a phenotype, enhancing a trait, increasing yield, increasing nitrogen use efficiency, or increasing water use efficiency in a plant comprising producing a transgenic plant comprising a recombinant DNA construct, wherein the recombinant DNA construct comprises: a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29; or a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92. The step of producing a transgenic plant may further comprise transforming a plant cell or tissue with the recombinant DNA construct, and regenerating or developing the transgenic plant from the plant cell or tissue comprising the recombinant DNA construct. The transgenic plant may then be crossed to (a) itself; (b) a second plant from the same plant line; (c) a wild type plant; or (d) a second plant from a different plant line, to produce one or more progeny plants; and a plant may be selected from the progeny plants having increased yield, increased nitrogen use efficiency, or increased water use efficiency as compared to a control plant. Plants produced by this method are further provided. According to some embodiments, the transgenic plant may be produced by site-directed integration of the recombinant DNA construct into the genome of a plant cell or tissue using a donor template comprising the recombinant DNA construct, and then regenerating or developing the transgenic plant from the transgenic plant cell or tissue.

[0006] According to another aspect, the present disclosure provides recombinant DNA molecules for use as a donor template in site-directed integration, wherein a recombinant DNA molecule comprises an insertion sequence comprising: a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29; or a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92. The insertion sequence of the recombinant DNA molecule may comprise a promoter, such as a heterologous promoter, functional in a plant cell and operably linked to the polynucleotide sequence. The recombinant DNA molecule may further comprise at least one homology arm flanking the insertion sequence. Plants, propagules and plant cells are further provided comprising the insertion sequence. According to some embodiments, the recombinant DNA molecule may further comprise an expression cassette encoding a site-specific nuclease and/or one or more guide RNAs.

[0007] According to another aspect, the present disclosure provides recombinant DNA molecules for use as a donor template in site-directed integration, wherein a recombinant DNA molecule comprises an insertion sequence for modulation of expression of an endogenous gene, wherein the endogenous gene comprises: a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29, or a portion thereof; or a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92. The insertion sequence may comprise a promoter, an enhancer, an intron, and/or a terminator region, which may correspond to a promoter, an enhancer, an intron, or a terminator region of an endogenous gene. Plants, propagules and plant cells are further provided comprising the insertion sequence. According to some embodiments, the recombinant DNA molecule may further comprise an expression cassette encoding a site-specific nuclease and/or one or more guide RNAs.

[0008] According to another aspect, the present disclosure provides methods for altering a phenotype, enhancing a trait, increasing yield, increasing nitrogen use efficiency, or increasing water use efficiency in a plant comprising: (a) modifying the genome of a plant cell by: (i) identifying an endogenous gene of the plant corresponding to a gene selected from the list of genes in Tables 1 and 12 herein, and their homologs, and (ii) modifying a sequence of the endogenous gene in the plant cell via site-directed integration to modify the expression level of the endogenous gene; and (b) regenerating or developing a plant from the plant cell.

DETAILED DESCRIPTION

[0009] In the attached sequence listing:

[0010] SEQ ID NOs 1 to 29 are nucleotide sequences of the coding strand of the DNA used in the recombinant DNA constructs imparting an enhanced trait in plants, each representing a coding sequence for a protein.

[0011] SEQ ID NOs 30 to 58 are amino acid sequences of the cognate proteins of the DNA molecules with nucleotide sequences of SEQ ID NOs 1 to 29 respectively in the same order.

[0012] SEQ ID NOs 59 to 92 are amino acid sequences of proteins homologous to the proteins with amino acid sequences of SEQ ID NOs 30 to 58.

[0013] SEQ ID NOs 93 to 101 are nucleotide sequences of DNA molecules used in the recombinant DNA constructs imparting an enhanced trait or altered phenotype in plants, each representing a promoter with a specific expression pattern.

[0014] Unless otherwise stated, nucleic acid sequences in the text of this specification are given, when read from left to right, in the 5' to 3' direction. One of skill in the art would be aware that a given DNA sequence is understood to define a corresponding RNA sequence which is identical to the DNA sequence except for replacement of the thymine (T) nucleotide of the DNA with uracil (U) nucleotide. Thus, providing a specific DNA sequence is understood to define the exact RNA equivalent. A given first polynucleotide sequence, whether DNA or RNA, further defines the sequence of its exact complement (which can be DNA or RNA), i.e., a second polynucleotide that hybridizes perfectly to the first polynucleotide by forming Watson-Crick base-pairs. By "essentially identical" or "essentially complementary" to a target gene or a fragment of a target gene is meant that a polynucleotide strand (or at least one strand of a double-stranded polynucleotide) is designed to hybridize (generally under physiological conditions such as those found in a living plant or animal cell) to a target gene or to a fragment of a target gene or to the transcript of the target gene or the fragment of a target gene; one of skill in the art would understand that such hybridization does not necessarily require 100% sequence identity or complementarity. A first nucleic acid sequence is "operably" connected or "linked" with a second nucleic acid sequence when the first nucleic acid sequence is placed in a functional relationship with the second nucleic acid sequence. For example, a promoter sequence is "operably linked" to a DNA sequence if the promoter provides for transcription or expression of the DNA sequence. Generally, operably linked DNA sequences are contiguous.

[0015] As used herein, the term "expression" refers to the production of a polynucleotide or a protein by a plant, plant cell or plant tissue which can give rise to an altered phenotype or enhanced trait. Expression can also refer to the process by which information from a gene is used in the synthesis of functional gene products, which may include but are not limited to other polynucleotides or proteins which may serve, e.g., an enzymatic, structural or regulatory function. Gene products having a regulatory function include but are not limited to elements that affect the occurrence or level of transcription or translation of a target protein. In some cases, the expression product is a non-coding functional RNA.

[0016] "Modulation" of expression refers to the process of effecting either overexpression or suppression of a polynucleotide or a protein.

[0017] The term "suppression" as used herein refers to a lower expression level of a target polynucleotide or target protein in a plant, plant cell or plant tissue, as compared to the expression in a wild-type or control plant, cell or tissue, at any developmental or temporal stage for the gene. The term "target protein" as used in the context of suppression refers to a protein which is suppressed; similarly, "target mRNA" refers to a polynucleotide which can be suppressed or, once expressed, degraded so as to result in suppression of the target protein it encodes. The term "target gene" as used in the context of suppression refers to either "target protein" or "target mRNA". In alternate non-limiting embodiments, suppression of the target protein or target polynucleotide can give rise to an enhanced trait or altered phenotype directly or indirectly. In one exemplary embodiment, the target protein is one which can indirectly increase or decrease the expression of one or more other proteins, the increased or decreased expression, respectively, of which is associated with an enhanced trait or an altered phenotype. In another exemplary embodiment, the target protein can bind to one or more other proteins associated with an altered phenotype or enhanced trait to enhance or inhibit their function and thereby affect the altered phenotype or enhanced trait indirectly.

[0018] Suppression can be applied using numerous approaches. Non-limiting examples include: suppressing an endogenous gene(s) or a subset of genes in a pathway, suppressing one or more mutation that has resulted in decreased activity of a protein, suppressing the production of an inhibitory agent, to elevate, reduce or eliminate the level of substrate that an enzyme requires for activity, producing a new protein, activating a normally silent gene; or accumulating a product that does not normally increase under natural conditions.

[0019] Conversely, the term "overexpression" as used herein refers to a greater expression level of a polynucleotide or a protein in a plant, plant cell or plant tissue, compared to expression in a wild-type plant, cell or tissue, at any developmental or temporal stage for the gene. Overexpression can take place in plant cells normally lacking expression of polypeptides functionally equivalent or identical to the present polypeptides. Overexpression can also occur in plant cells where endogenous expression of the present polypeptides or functionally equivalent molecules normally occurs, but such normal expression is at a lower level. Overexpression thus results in a greater than normal production, or "overproduction" of the polypeptide in the plant, cell or tissue.

[0020] The term "target protein" as used herein in the context of overexpression refers to a protein which is overexpressed; "target mRNA" refers to an mRNA which encodes and is translated to produce the target protein, which can also be overexpressed. The term "target gene" as used in the context of overexpression refers to either "target protein" or "target mRNA". In alternative embodiments, the target protein can effect an enhanced trait or altered phenotype directly or indirectly. In the latter case it may do so, for example, by affecting the expression, function or substrate available to one or more other proteins. In an exemplary embodiment, the target protein can bind to one or more other proteins associated with an altered phenotype or enhanced trait to enhance or inhibit their function.

[0021] Overexpression can be achieved using numerous approaches. In one embodiment, overexpression can be achieved by placing the DNA sequence encoding one or more polynucleotides or polypeptides under the control of a promoter, examples of which include but are not limited to endogenous promoters, heterologous promoters, inducible promoters, development specific promoters, and tissue specific promoters. In one exemplary embodiment, the promoter is a constitutive promoter, for example, the cauliflower mosaic virus 35S promoter and other constitutive promoters known in the art. Thus, depending on the promoter used, overexpression can occur throughout a plant, in specific tissues of the plant, in specific stages of development of the plant, or in the presence or absence of different inducing or inducible agents, such as hormones or environmental signals.

[0022] Gene Suppression Elements: The gene suppression element can be transcribable DNA of any suitable length, and generally includes at least about 19 to about 27 nucleotides (for example 19, 20, 21, 22, 23, or 24 nucleotides) for every target gene that the recombinant DNA construct is intended to suppress. In many embodiments, the gene suppression element includes more than 23 nucleotides (for example, more than about 30, about 50, about 100, about 200, about 300, about 500, about 1000, about 1500, about 2000, about 3000, about 4000, or about 5000 nucleotides) for every target gene that the recombinant DNA construct is intended to suppress.

[0023] Suitable gene suppression elements useful in the recombinant DNA constructs of the invention include at least one element (and, in some embodiments, multiple elements) selected from the group consisting of: (a) DNA that includes at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one first target gene; (b) DNA that includes multiple copies of at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one first target gene; (c) DNA that includes at least one sense DNA segment that is at least one segment of the at least one first target gene; (d) DNA that includes multiple copies of at least one sense DNA segment that is at least one segment of the at least one first target gene; (e) DNA that transcribes to RNA for suppressing the at least one first target gene by forming double-stranded RNA and includes at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one target gene and at least one sense DNA segment that is at least one segment of the at least one first target gene; (f) DNA that transcribes to RNA for suppressing the at least one first target gene by forming a single double-stranded RNA and includes multiple serial anti-sense DNA segments that are anti-sense to at least one segment of the at least one first target gene and multiple serial sense DNA segments that are at least one segment of the at least one first target gene; (g) DNA that transcribes to RNA for suppressing the at least one first target gene by forming multiple double strands of RNA and includes multiple anti-sense DNA segments that are anti-sense to at least one segment of the at least one first target gene and multiple sense DNA segments that are at least one segment of the at least one first target gene, and wherein the multiple anti-sense DNA segments and the multiple sense DNA segments are arranged in a series of inverted repeats; (h) DNA that includes nucleotides derived from a miRNA, preferably a plant miRNA; (i) DNA that includes nucleotides of a siRNA; (j) DNA that transcribes to an RNA aptamer capable of binding to a ligand; and (k) DNA that transcribes to an RNA aptamer capable of binding to a ligand, and DNA that transcribes to regulatory RNA capable of regulating expression of the first target gene, wherein the regulation is dependent on the conformation of the regulatory RNA, and the conformation of the regulatory RNA is allosterically affected by the binding state of the RNA aptamer.

[0024] Any of these gene suppression elements, whether transcribing to a single double-stranded RNA or to multiple double-stranded RNAs, can be designed to suppress more than one target gene, including, for example, more than one allele of a target gene, multiple target genes (or multiple segments of at least one target gene) from a single species, or target genes from different species.

[0025] Anti-Sense DNA Segments: In one embodiment, the at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one first target gene includes DNA sequence that is anti-sense or complementary to at least a segment of the at least one first target gene, and can include multiple anti-sense DNA segments, that is, multiple copies of at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one first target gene. Multiple anti-sense DNA segments can include DNA sequence that is anti-sense or complementary to multiple segments of the at least one first target gene, or to multiple copies of a segment of the at least one first target gene, or to segments of multiple first target genes, or to any combination of these. Multiple anti-sense DNA segments can be fused into a chimera, e.g., including DNA sequences that are anti-sense to multiple segments of one or more first target genes and fused together.

[0026] The anti-sense DNA sequence that is anti-sense or complementary to (that is, can form Watson-Crick base-pairs with) at least a segment of the at least one first target gene has at least about 80%, or at least about 85%, or at least about 90%, or at least about 95% complementarity to at least a segment of the at least one first target gene. In one embodiment, the DNA sequence that is anti-sense or complementary to at least a segment of the at least one first target gene has between about 95% to about 100% complementarity to at least a segment of the at least one first target gene. Where the at least one anti-sense DNA segment includes multiple anti-sense DNA segments, the degree of complementarity can be, but need not be, identical for all of the multiple anti-sense DNA segments.

[0027] Sense DNA Segments: In another embodiment, the at least one sense DNA segment that is at least one segment of the at least one first target gene includes DNA sequence that corresponds to (that is, has a sequence that is identical or substantially identical to) at least a segment of the at least one first target gene, and can include multiple sense DNA segments, that is, multiple copies of at least one sense DNA segment that corresponds to (that is, has the nucleotide sequence of) at least one segment of the at least one first target gene. Multiple sense DNA segments can include DNA sequence that is or that corresponds to multiple segments of the at least one first target gene, or to multiple copies of a segment of the at least one first target gene, or to segments of multiple first target genes, or to any combination of these. Multiple sense DNA segments can be fused into a chimera, that is, can include DNA sequences corresponding to multiple segments of one or more first target genes and fused together.

[0028] The sense DNA sequence that corresponds to at least a segment of the target gene has at least about 80%, or at least about 85%, or at least about 90%, or at least about 95% sequence identity to at least a segment of the target gene. In one embodiment, the DNA sequence that corresponds to at least a segment of the target gene has between about 95% to about 100% sequence identity to at least a segment of the target gene. Where the at least one sense DNA segment includes multiple sense DNA segments, the degree of sequence identity can be, but need not be, identical for all of the multiple sense DNA segments.

[0029] Multiple Copies: Where the gene suppression element includes multiple copies of anti-sense or multiple copies of sense DNA sequence, these multiple copies can be arranged serially in tandem repeats. In some embodiments, these multiple copies can be arranged serially end-to-end, that is, in directly connected tandem repeats. In some embodiments, these multiple copies can be arranged serially in interrupted tandem repeats, where one or more spacer DNA segment can be located adjacent to one or more of the multiple copies. Tandem repeats, whether directly connected or interrupted or a combination of both, can include multiple copies of a single anti-sense or multiple copies of a single sense DNA sequence in a serial arrangement or can include multiple copies of more than one anti-sense DNA sequence or of more than one sense DNA sequence in a serial arrangement.

[0030] Double-stranded RNA: In those embodiments wherein the gene suppression element includes either at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one target gene or at least one sense DNA segment that is at least one segment of the at least one target gene, RNA transcribed from either the at least one anti-sense or at least one sense DNA may become double-stranded by the action of an RNA-dependent RNA polymerase. See, for example, U.S. Pat. No. 5,283,184, which is incorporated by reference herein.

[0031] In yet other embodiments, the gene suppression element can include DNA that transcribes to RNA for suppressing the at least one first target gene by forming double-stranded RNA and includes at least one anti-sense DNA segment that is anti-sense to at least one segment of the at least one target gene (as described above under the heading "Anti-sense DNA Segments") and at least one sense DNA segment that is at least one segment of the at least one first target gene (as described above under the heading "Sense DNA Segments"). Such a gene suppression element can further include spacer DNA segments. Each at least one anti-sense DNA segment is complementary to at least part of a sense DNA segment in order to permit formation of double-stranded RNA by intramolecular hybridization of the at least one anti-sense DNA segment and the at least one sense DNA segment. Such complementarity between an anti-sense DNA segment and a sense DNA segment can be, but need not be, 100% complementary; in some embodiments, this complementarity can be preferably at least about 80%, or at least about 85%, or at least about 90%, or at least about 95% complementary.

[0032] The double-stranded RNA can be in the form of a single dsRNA "stem" (region of base-pairing between sense and anti-sense strands), or can have multiple dsRNA "stems." In one embodiment, the gene suppression element can include DNA that transcribes to RNA for suppressing the at least one first target gene by forming essentially a single double-stranded RNA and includes multiple serial anti-sense DNA segments that are anti-sense to at least one segment of the at least one first target gene and multiple serial sense DNA segments that are at least one segment of the at least one first target gene; the multiple serial anti-sense and multiple serial sense segments can form a single double-stranded RNA "stem" or multiple "stems" in a serial arrangement (with or without non-base paired spacer DNA separating the multiple "stems"). In another embodiment, the gene suppression element includes DNA that transcribes to RNA for suppressing the at least one first target gene by forming multiple dsRNA "stems" of RNA and includes multiple anti-sense DNA segments that are anti-sense to at least one segment of the at least one first target gene and multiple sense DNA segments that are at least one segment of the at least one first target gene, and wherein the multiple anti-sense DNA segments and the multiple sense DNA segments are arranged in a series of dsRNA "stems" (such as, but not limited to "inverted repeats"). Such multiple dsRNA "stems" can further be arranged in series or clusters to form tandem inverted repeats, or structures resembling "hammerhead" or "cloverleaf" shapes. Any of these gene suppression elements can further include spacer DNA segments found within a dsRNA "stem" (for example, as a spacer between multiple anti-sense or sense DNA segments or as a spacer between a base-pairing anti-sense DNA segment and a sense DNA segment) or outside of a double-stranded RNA "stem" (for example, as a loop region separating a pair of inverted repeats). In cases where base-pairing anti-sense and sense DNA segments are of unequal length, the longer segment can act as a spacer.

[0033] miRNAs: In a further embodiment, the gene suppression element can include DNA that includes nucleotides derived from a miRNA (microRNA), that is, a DNA sequence that corresponds to a miRNA native to a virus or a eukaryote of interest (including plants and animals, especially invertebrates), or a DNA sequence derived from such a native miRNA but modified to include nucleotide sequences that do not correspond to the native miRNA. While miRNAs have not to date been reported in fungi, fungal miRNAs, should they exist, are also suitable for use in the invention. An embodiment includes a gene suppression element containing DNA that includes nucleotides derived from a viral or plant miRNA.

[0034] In a non-limiting example, the nucleotides derived from a miRNA can include DNA that includes nucleotides corresponding to the loop region of a native miRNA and nucleotides that are selected from a target gene sequence. In another non-limiting example, the nucleotides derived from a miRNA can include DNA derived from a miRNA precursor sequence, such as a native pri-miRNA or pre-miRNA sequence, or nucleotides corresponding to the regions of a native miRNA and nucleotides that are selected from a target gene sequence number such that the overall structure (e.g., the placement of mismatches in the stem structure of the pre-miRNA) is preserved to permit the pre-miRNA to be processed into a mature miRNA. In yet another embodiment, the gene suppression element can include DNA that includes nucleotides derived from a miRNA and capable of inducing or guiding in-phase cleavage of an endogenous transcript into trans-acting siRNAs, as described by Allen et al. (2005) Cell, 121:207-221. Thus, the DNA that includes nucleotides derived from a miRNA can include sequence naturally occurring in a miRNA or a miRNA precursor molecule, synthetic sequence, or both.

[0035] siRNAs: In yet another embodiment, the gene suppression element can include DNA that includes nucleotides of a small interfering RNA (siRNA). The siRNA can be one or more native siRNAs (such as siRNAs isolated from a non-transgenic eukaryote or from a transgenic eukaryote), or can be one or more DNA sequences predicted to have siRNA activity (such as by use of predictive tools known in the art, see, for example, Reynolds et al. (2004) Nature Biotechnol., 22:326-330). Multiple native or predicted siRNA sequences can be joined in a chimeric siRNA sequence for gene suppression. Such a DNA that includes nucleotides of a siRNA includes at least 19 nucleotides, and in some embodiments includes at least 20, at least 21, at least 22, at least 23, or at least 24 nucleotides. In other embodiments, the DNA that includes nucleotides of a siRNA can contain substantially more than 21 nucleotides, for example, more than about 50, about 100, about 300, about 500, about 1000, about 3000, or about 5000 nucleotides or greater.

[0036] Engineered miRNAs and trans-acting siRNAs (ta-siRNAs) are useful for gene suppression with increased specificity. The invention provides recombinant DNA constructs, each including a transcribable engineered miRNA precursor designed to suppress a target sequence, wherein the transcribable engineered miRNA precursor is derived from the fold-back structure of a MIR gene, preferably a plant MIR sequence. These miRNA precursors are also useful for directing in-phase production of siRNAs (e.g., heterologous sequence designed to be processed in a trans-acting siRNA suppression mechanism in planta). The invention further provides a method to suppress expression of a target sequence in a plant cell, including transcribing in a plant cell a recombinant DNA including a transcribable engineered miRNA precursor designed to suppress a target sequence, wherein the transcribable engineered miRNA precursor is derived from the fold-back structure of a MIR gene, preferably a plant MIR sequence, whereby expression of the target sequence is suppressed relative to its expression in the absence of transcription of the recombinant DNA construct.

[0037] The mature miRNAs produced, or predicted to be produced, from these miRNA precursors may be engineered for use in suppression of a target gene, e.g., in transcriptional suppression by the miRNA, or to direct in-phase production of siRNAs in a trans-acting siRNA suppression mechanism (see Allen et al. (2005) Cell, 121:207-221, Vaucheret (2005) Science STKE, 2005:pe43, and Yoshikawa et al. (2005) Genes Dev., 19:2164-2175). Plant miRNAs generally have near-perfect complementarity to their target sequences (see, for example, Llave et al. (2002) Science, 297:2053-2056, Rhoades et al. (2002) Cell, 110:513-520, Jones-Rhoades and Bartel (2004) Mol. Cell, 14:787-799). Thus, the mature miRNAs can be engineered to serve as sequences useful for gene suppression of a target sequence, by replacing nucleotides of the mature miRNA sequence with nucleotides of the sequence that is targeted for suppression; see, for example, methods disclosed by Parizotto et al. (2004) Genes Dev., 18:2237-2242 and especially U.S. Patent Application Publications US2004/0053411A1, US2004/0268441A1, US2005/0144669, and US2005/0037988, all of which are incorporated by reference herein. When engineering a novel miRNA to target a specific sequence, one strategy is to select within the target sequence a region with sequence that is as similar as possible to the native miRNA sequence. Alternatively, the native miRNA sequence can be replaced with a region of the target sequence, preferably a region that meets structural and thermodynamic criteria believed to be important for miRNA function (see, for example, U.S. Patent Application Publication US2005/0037988). Sequences are preferably engineered such that the number and placement of mismatches in the stem structure of the fold-back region or pre-miRNA is preserved. Thus, an engineered miRNA or engineered miRNA precursor can be derived from any of the mature miRNA sequences, or their corresponding miRNA precursors (including the fold-back portions of the corresponding MIR genes) disclosed herein. The engineered miRNA precursor can be cloned and expressed (transiently or stably) in a plant cell or tissue or intact plant.

[0038] The construction and description of recombinant DNA constructs to modulate small non-coding RNA activities are disclosed in U.S. Patent Application Publication US 2009/0070898 A1, US2011/0296555 A1,US2011/0035839 A1, all of which are incorporated herein by reference in their entirety. In particular, with respect to US2011/0035839 A1, see e.g., sections under the headings "Gene Suppression Elements" in paragraphs 122 to 135, and "Engineered Heterologous miRNA for Controlling Gene Expression in paragraphs 188 to 190.

[0039] As used herein a "plant" includes a whole plant, a transgenic plant, meristematic tissue, a shoot organ/structure (for example, leaf, stem and tuber), a root, a flower, a floral organ/structure (for example, a bract, a sepal, a petal, a stamen, a carpel, an anther and an ovule), a seed (including an embryo, endosperm, and a seed coat) and a fruit (the mature ovary), plant tissue (for example, vascular tissue, ground tissue, and the like) and a cell (for example, guard cell, egg cell, pollen, mesophyll cell, and the like), and progeny of same. The classes of plants that can be used in the disclosed methods are generally as broad as the classes of higher and lower plants amenable to transformation and breeding techniques, including angiosperms (monocotyledonous and dicotyledonous plants), gymnosperms, ferns, horsetails, psilophytes, lycophytes, bryophytes, and multicellular algae.

[0040] As used herein a "transgenic plant cell" means a plant cell that is transformed with stably-integrated, recombinant DNA, for example, by Agrobacterium-mediated transformation, by bombardment using microparticles coated with recombinant DNA, or by other means, such as site-directed integration. A plant cell of this disclosure can be an originally-transformed plant cell that exists as a microorganism or as a progeny plant cell that is regenerated into differentiated tissue, for example, into a transgenic plant with stably-integrated, recombinant DNA, or seed or pollen derived from a progeny transgenic plant.

[0041] As used herein a "control plant" means a plant that does not contain the recombinant DNA of the present disclosure that imparts an enhanced trait or altered phenotype. A control plant is used to identify and select a transgenic plant that has an enhanced trait or altered phenotype. A suitable control plant can be a non-transgenic plant of the parental line used to generate a transgenic plant, for example, a wild type plant devoid of a recombinant DNA. A suitable control plant can also be a transgenic plant that contains recombinant DNA that imparts other traits, for example, a transgenic plant having enhanced herbicide tolerance. A suitable control plant can in some cases be a progeny of a hemizygous transgenic plant line that does not contain the recombinant DNA, known as a negative segregant, or a negative isogenic line.

[0042] As used herein a "propagule" includes all products of meiosis and mitosis, including but not limited to, plant, seed and part of a plant able to propagate a new plant. Propagules include whole plants, cells, pollen, ovules, flowers, embryos, leaves, roots, stems, shoots, meristems, grains or seeds, or any plant part that is capable of growing into an entire plant. Propagule also includes graft where one portion of a plant is grafted to another portion of a different plant (even one of a different species) to create a living organism. Propagule also includes all plants and seeds produced by cloning or by bringing together meiotic products, or allowing meiotic products to come together to form an embryo or a fertilized egg (naturally or with human intervention).

[0043] As used herein a "progeny" includes any plant, seed, plant cell, and/or regenerable plant part comprising a recombinant DNA of the present disclosure derived from an ancestor plant. A progeny can be homozygous or heterozygous for the transgene. Progeny can be grown from seeds produced by a transgenic plant comprising a recombinant DNA of the present disclosure, and/or from seeds produced by a plant fertilized with pollen or ovule from a transgenic plant comprising a recombinant DNA of the present disclosure.

[0044] As used herein a "trait" is a physiological, morphological, biochemical, or physical characteristic of a plant or particular plant material or cell. In some instances, this characteristic is visible to the human eye and can be measured mechanically, such as seed or plant size, weight, shape, form, length, height, growth rate and development stage, or can be measured by biochemical techniques, such as detecting the protein, starch, certain metabolites, or oil content of seed or leaves, or by observation of a metabolic or physiological process, for example, by measuring tolerance to water deprivation or particular salt or sugar concentrations, or by the measurement of the expression level of a gene or genes, for example, by employing Northern analysis, RT-PCR, microarray gene expression assays, or reporter gene expression systems, or by agricultural observations such as hyperosmotic stress tolerance or yield. Any technique can be used to measure the amount of, comparative level of, or difference in any selected chemical compound or macromolecule in the transgenic plants, however.

[0045] As used herein an "enhanced trait" means a characteristic of a transgenic plant as a result of stable integration and expression of a recombinant DNA in the transgenic plant. Such traits include, but are not limited to, an enhanced agronomic trait characterized by enhanced plant morphology, physiology, growth and development, yield, nutritional enhancement, disease or pest resistance, or environmental or chemical tolerance. In some specific aspects of this disclosure an enhanced trait is selected from the group consisting of decreased days from planting to maturity, increased stalk size, increased number of leaves, increased plant height growth rate in vegetative stage, increased ear size, increased ear dry weight per plant, increased number of kernels per ear, increased weight per kernel, increased number of kernels per plant, decreased ear void, extended grain fill period, reduced plant height, increased number of root branches, increased total root length, drought tolerance, increased water use efficiency, cold tolerance, increased nitrogen use efficiency and increased yield as shown in Tables 7-10, and altered phenotypes as shown in Tables 3-5. In another aspect of the disclosure the trait is increased yield under non-stress conditions or increased yield under environmental stress conditions. Stress conditions can include both biotic and abiotic stress, for example, drought, shade, fungal disease, viral disease, bacterial disease, insect infestation, nematode infestation, cold temperature exposure, heat exposure, osmotic stress, reduced nitrogen nutrient availability, reduced phosphorus nutrient availability and high plant density. "Yield" can be affected by many properties including without limitation, plant height, plant biomass, pod number, pod position on the plant, number of internodes, incidence of pod shatter, grain size, ear size, ear tip filling, kernel abortion, efficiency of nodulation and nitrogen fixation, efficiency of nutrient assimilation, resistance to biotic and abiotic stress, carbon assimilation, plant architecture, resistance to lodging, percent seed germination, seedling vigor, and juvenile traits. Yield can also be affected by efficiency of germination (including germination in stressed conditions), growth rate (including growth rate in stressed conditions), flowering time and duration, ear number, ear size, ear weight, seed number per ear or pod, seed size, composition of seed (starch, oil, protein) and characteristics of seed fill.

[0046] Also used herein, the term "trait modification" encompasses altering the naturally occurring trait by producing a detectable difference in a characteristic in a plant comprising a recombinant DNA of the present disclosure relative to a plant not comprising the recombinant DNA, such as a wild-type plant, or a negative segregant. In some cases, the trait modification can be evaluated quantitatively. For example, the trait modification can entail an increase or decrease, in an observed trait characteristics or phenotype as compared to a control plant. It is known that there can be natural variations in a modified trait. Therefore, the trait modification observed entails a change of the normal distribution and magnitude of the trait characteristics or phenotype in the plants as compared to a control plant.

[0047] The present disclosure relates to a plant with improved economically important characteristics, more specifically increased yield. More specifically the present disclosure relates to a plant comprising a polynucleotide of this disclosure, wherein the plant has increased yield as compared to a control plant. Many plants of this disclosure exhibited increased yield as compared to a control plant. In an embodiment, a plant of the present disclosure exhibited an improved trait that is related to yield, including but not limited to increased nitrogen use efficiency, increased nitrogen stress tolerance, increased water use efficiency and increased drought tolerance, as defined and discussed infra.

[0048] Yield can be defined as the measurable produce of economic value from a crop. Yield can be defined in the scope of quantity and/or quality. Yield can be directly dependent on several factors, for example, the number and size of organs, plant architecture (such as the number of branches, plant biomass, etc.), flowering time and duration, grain fill period. Root architecture and development, photosynthetic efficiency, nutrient uptake, stress tolerance, early vigor, delayed senescence and functional stay green phenotypes can be important factors in determining yield. Optimizing the above mentioned factors can therefore contribute to increasing crop yield.

[0049] Reference herein to an increase in yield-related traits can also be taken to mean an increase in biomass (weight) of one or more parts of a plant, which can include above ground and/or below ground (harvestable) plant parts. In particular, such harvestable parts are seeds, and performance of the methods of the disclosure results in plants with increased yield and in particular increased seed yield relative to the seed yield of suitable control plants. The term "yield" of a plant can relate to vegetative biomass (root and/or shoot biomass), to reproductive organs, and/or to propagules (such as seeds) of that plant.

[0050] Increased yield of a plant of the present disclosure can be measured in a number of ways, including test weight, seed number per plant, seed weight, seed number per unit area (for example, seeds, or weight of seeds, per acre), bushels per acre, tons per acre, or kilo per hectare. For example, corn yield can be measured as production of shelled corn kernels per unit of production area, for example in bushels per acre or metric tons per hectare. This is often also reported on a moisture adjusted basis, for example at 15.5 percent moisture. Increased yield can result from improved utilization of key biochemical compounds, such as nitrogen, phosphorous and carbohydrate, or from improved responses to environmental stresses, such as cold, heat, drought, salt, shade, high plant density, and attack by pests or pathogens. This disclosure can also be used to provide plants with improved growth and development, and ultimately increased yield, as the result of modified expression of plant growth regulators or modification of cell cycle or photosynthesis pathways. Also of interest is the generation of plants that demonstrate increased yield with respect to a seed component that may or may not correspond to an increase in overall plant yield.

[0051] In an embodiment, "alfalfa yield" can also be measured in forage yield, the amount of above ground biomass at harvest. Factors leading contributing to increased biomass include increased vegetative growth, branches, nodes and internodes, leaf area, and leaf area index.

[0052] In another embodiment, "canola yield" can also be measured in pod number, number of pods per plant, number of pods per node, number of internodes, incidence of pod shatter, seeds per silique, seed weight per silique, improved seed, oil, or protein composition.

[0053] Additionally, "corn or maize yield" can also be measured as production of shelled corn kernels per unit of production area, ears per acre, number of kernel rows per ear and number of kernels per row, kernel number or weight per ear, weight per kernel, ear number, ear weight, fresh or dry ear biomass (weight)

[0054] In yet another embodiment, "cotton yield" can be measured as bolls per plant, size of bolls, fiber quality, seed cotton yield in g/plant, seed cotton yield in lb/acre, lint yield in lb/acre, and number of bales.

[0055] Specific embodiment for "rice yield" can also include panicles per hill, grain per hill, and filled grains per panicle.

[0056] Still further embodiment for "soybean yield" can also include pods per plant, pods per acre, seeds per plant, seeds per pod, weight per seed, weight per pod, pods per node, number of nodes, and the number of internodes per plant.

[0057] In still further embodiment, "sugarcane yield" can be measured as cane yield (tons per acre; kg/hectare), total recoverable sugar (pounds per ton), and sugar yield (tons/acre).

[0058] In yet still further embodiment, "wheat yield" can include: cereal per unit area, grain number, grain weight, grain size, grains per head, seeds per head, seeds per plant, heads per acre, number of viable tillers per plant, composition of seed (for example, carbohydrates, starch, oil, and protein) and characteristics of seed fill.

[0059] The terms "yield", "seed yield" are defined above for a number of core crops. The terms "increased", "improved", "enhanced" are interchangeable and are defined herein.

[0060] In another embodiment, the present disclosure provides a method for the production of plants having altered phenotype, enhanced trait, or increased yield; performance of the method gives plants altered phenotype, enhanced trait, or increased yield.

[0061] "Increased yield" can manifest as one or more of the following: (i) increased plant biomass (weight) of one or more parts of a plant, particularly aboveground (harvestable) parts, of a plant, increased root biomass (increased number of roots, increased root thickness, increased root length) or increased biomass of any other harvestable part; or (ii) increased early vigor, defined herein as an improved seedling aboveground area approximately three weeks post-germination. "Early vigor" refers to active healthy plant growth especially during early stages of plant growth, and can result from increased plant fitness due to, for example, the plants being better adapted to their environment (for example, optimizing the use of energy resources, uptake of nutrients and partitioning carbon allocation between shoot and root). Early vigor in corn, for example, is a combination of the ability of corn seeds to germinate and emerge after planting and the ability of the young corn plants to grow and develop after emergence. Plants having early vigor also show increased seedling survival and better establishment of the crop, which often results in highly uniform fields with the majority of the plants reaching the various stages of development at substantially the same time, which often results in increased yield. Therefore early vigor can be determined by measuring various factors, such as kernel weight, percentage germination, percentage emergence, seedling growth, seedling height, root length, root and shoot biomass, canopy size and color and others.

[0062] Further, increased yield can also manifest as (iii) increased total seed yield, which may result from one or more of an increase in seed biomass (seed weight) due to an increase in the seed weight on a per plant and/or on an individual seed basis an increased number of panicles per plant; an increased number of pods; an increased number of nodes; an increased number of flowers ("florets") per panicle/plant; increased seed fill rate; an increased number of filled seeds; increased seed size (length, width, area, perimeter), which can also influence the composition of seeds; and/or increased seed volume, which can also influence the composition of seeds.

[0063] Increased yield can also (iv) result in modified architecture, or can occur because of modified plant architecture.

[0064] Increased yield can also manifest as (v) increased harvest index, which is expressed as a ratio of the yield of harvestable parts, such as seeds, over the total biomass

[0065] Increased yield can also manifest as (vi) increased kernel weight, which is extrapolated from the number of filled seeds counted and their total weight. An increased kernel weight can result from an increased seed size and/or seed weight, an increase in embryo size, increased endosperm size, aleurone and/or scutellum, or an increase with respect to other parts of the seed that result in increased kernel weight.

[0066] Increased yield can also manifest as (vii) increased ear biomass, which is the weight of the ear and can be represented on a per ear, per plant or per plot basis.

[0067] In one embodiment, increased yield can be increased seed yield, and is selected from one of the following: (i) increased seed weight; (ii) increased number of filled seeds; and (iii) increased harvest index.

[0068] The disclosure also extends to harvestable parts of a plant such as, but not limited to, seeds, leaves, fruits, flowers, bolls, stems, rhizomes, tubers and bulbs. The disclosure furthermore relates to products derived from a harvestable part of such a plant, such as dry pellets, powders, oil, fat and fatty acids, starch or proteins.

[0069] The present disclosure provides a method for increasing "yield" of a plant or "broad acre yield" of a plant or plant part defined as the harvestable plant parts per unit area, for example seeds, or weight of seeds, per acre, pounds per acre, bushels per acre, tones per acre, tons per acre, kilo per hectare.

[0070] This disclosure further provides a method of altering phenotype, enhancing trait, or increasing yield in a plant by producing a plant comprising a polynucleotide sequence of this disclosure where the plant can be crossed with itself, a second plant from the same plant line, a wild type plant, or a plant from a different line of plants to produce a seed. The seed of the resultant plant can be harvested from fertile plants and be used to grow progeny generations of plant(s) of this disclosure. In addition to direct transformation of a plant with a recombinant DNA construct, transgenic plants can be prepared by crossing a first plant having a stably integrated recombinant DNA construct with a second plant lacking the DNA. For example, recombinant DNA can be introduced into a first plant line that is amenable to transformation to produce a transgenic plant which can be crossed with a second plant line to introgress the recombinant DNA into the second plant line.

[0071] Selected transgenic plants transformed with a recombinant DNA construct and having the polynucleotide of this disclosure provides the altered phenotype, enhanced trait, or increased yield compared to a control plant. Use of genetic markers associated with the recombinant DNA can facilitate production of transgenic progeny that is homozygous for the desired recombinant DNA. Progeny plants carrying DNA for both parental traits can be back-crossed into a parent line multiple times, for example usually 6 to 8 generations, to produce a progeny plant with substantially the same genotype as the one reoccurring original transgenic parental line but having the recombinant DNA of the other transgenic parental line. The term "progeny" denotes the offspring of any generation of a parent plant prepared by the methods of this disclosure containing the recombinant polynucleotides as described herein.

[0072] As used herein "nitrogen use efficiency" refers to the processes which lead to an increase in the plant's yield, biomass, vigor, and growth rate per nitrogen unit applied. The processes can include the uptake, assimilation, accumulation, signaling, sensing, retranslocation (within the plant) and use of nitrogen by the plant.

[0073] As used herein "nitrogen limiting conditions" refers to growth conditions or environments that provide less than optimal amounts of nitrogen needed for adequate or successful plant metabolism, growth, reproductive success and/or viability.

[0074] As used herein the "increased nitrogen stress tolerance" refers to the ability of plants to grow, develop, or yield normally, or grow, develop, or yield faster or better when subjected to less than optimal amounts of available/applied nitrogen, or under nitrogen limiting conditions.

[0075] As used herein "increased nitrogen use efficiency" refers to the ability of plants to grow, develop, or yield faster or better than normal when subjected to the same amount of available/applied nitrogen as under normal or standard conditions; ability of plants to grow, develop, or yield normally, or grow, develop, or yield faster or better when subjected to less than optimal amounts of available/applied nitrogen, or under nitrogen limiting conditions.

[0076] Increased plant nitrogen use efficiency can be translated in the field into either harvesting similar quantities of yield, while supplying less nitrogen, or increased yield gained by supplying optimal/sufficient amounts of nitrogen. The increased nitrogen use efficiency can improve plant nitrogen stress tolerance, and can also improve crop quality and biochemical constituents of the seed such as protein yield and oil yield. The terms "increased nitrogen use efficiency", "enhanced nitrogen use efficiency", and "nitrogen stress tolerance" are used inter-changeably in the present disclosure to refer to plants with improved productivity under nitrogen limiting conditions.

[0077] As used herein "water use efficiency" refers to the amount of carbon dioxide assimilated by leaves per unit of water vapor transpired. It constitutes one of the most important traits controlling plant productivity in dry environments. "Drought tolerance" refers to the degree to which a plant is adapted to arid or drought conditions. The physiological responses of plants to a deficit of water include leaf wilting, a reduction in leaf area, leaf abscission, and the stimulation of root growth by directing nutrients to the underground parts of the plants. Plants are more susceptible to drought during flowering and seed development (the reproductive stages), as plant's resources are deviated to support root growth. In addition, abscisic acid (ABA), a plant stress hormone, induces the closure of leaf stomata (microscopic pores involved in gas exchange), thereby reducing water loss through transpiration, and decreasing the rate of photosynthesis. These responses improve the water-use efficiency of the plant on the short term. The terms "increased water use efficiency", "enhanced water use efficiency", and "increased drought tolerance" are used inter-changeably in the present disclosure to refer to plants with improved productivity under water-limiting conditions.

[0078] As used herein "increased water use efficiency" refers to the ability of plants to grow, develop, or yield faster or better than normal when subjected to the same amount of available/applied water as under normal or standard conditions; ability of plants to grow, develop, or yield normally, or grow, develop, or yield faster or better when subjected to reduced amounts of available/applied water (water input) or under conditions of water stress or water deficit stress.

[0079] As used herein "increased drought tolerance" refers to the ability of plants to grow, develop, or yield normally, or grow, develop, or yield faster or better than normal when subjected to reduced amounts of available/applied water and/or under conditions of acute or chronic drought; ability of plants to grow, develop, or yield normally when subjected to reduced amounts of available/applied water (water input) or under conditions of water deficit stress or under conditions of acute or chronic drought.

[0080] As used herein "drought stress" refers to a period of dryness (acute or chronic/prolonged) that results in water deficit and subjects plants to stress and/or damage to plant tissues and/or negatively affects grain/crop yield; a period of dryness (acute or chronic/prolonged) that results in water deficit and/or higher temperatures and subjects plants to stress and/or damage to plant tissues and/or negatively affects grain/crop yield.

[0081] As used herein "water deficit" refers to the conditions or environments that provide less than optimal amounts of water needed for adequate/successful growth and development of plants.

[0082] As used herein "water stress" refers to the conditions or environments that provide improper (either less/insufficient or more/excessive) amounts of water than that needed for adequate/successful growth and development of plants/crops thereby subjecting the plants to stress and/or damage to plant tissues and/or negatively affecting grain/crop yield.

[0083] As used herein "water deficit stress" refers to the conditions or environments that provide less/insufficient amounts of water than that needed for adequate/successful growth and development of plants/crops thereby subjecting the plants to stress and/or damage to plant tissues and/or negatively affecting grain yield.

[0084] As used herein a "polynucleotide" is a nucleic acid molecule comprising a plurality of polymerized nucleotides. A polynucleotide may be referred to as a nucleic acid, a oligonucleotide, or any fragment thereof. In many instances, a polynucleotide encodes a polypeptide (or protein) or a domain or a fragment thereof. Additionally, a polynucleotide can comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5' or 3' untranslated regions, a reporter gene, a selectable marker, a scorable marker, or the like. A polynucleotide can be single-stranded or double-stranded DNA or RNA. A polynucleotide optionally comprises modified bases or a modified backbone. A polynucleotide can be, for example, genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. A polynucleotide can be combined with carbohydrate(s), lipid(s), protein(s), or other materials to perform a particular activity, such as transformation, or form a composition such as a peptide nucleic acid (PNA). A polynucleotide can comprise a sequence in either sense or antisense orientations. "Oligonucleotide" is substantially equivalent to the terms amplimer, primer, oligomer, element, target, and probe and is preferably single-stranded.

[0085] As used herein a "recombinant polynucleotide" or "recombinant DNA" is a polynucleotide that is not in its native state, for example, a polynucleotide comprises a series of nucleotides (represented as a nucleotide sequence) not found in nature, or a polynucleotide is in a context other than that in which it is naturally found; for example, separated from polynucleotides with which it typically is in proximity in nature, or adjacent (or contiguous with) polynucleotides with which it typically is not in proximity The "recombinant polynucleotide" or "recombinant DNA" refers to polynucleotide or DNA which has been genetically engineered and constructed outside of a cell including DNA containing naturally occurring DNA or cDNA or synthetic DNA. For example, the polynucleotide at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acids.

[0086] As used herein a "polypeptide" comprises a plurality of consecutive polymerized amino acid residues for example, at least about 15 consecutive polymerized amino acid residues. In many instances, a polypeptide comprises a series of polymerized amino acid residues that is a transcriptional regulator or a domain or portion or fragment thereof. Additionally, the polypeptide can comprise: (i) a localization domain; (ii) an activation domain; (iii) a repression domain; (iv) an oligomerization domain; (v) a protein-protein interaction domain; (vi) a DNA-binding domain; or the like. The polypeptide optionally comprises modified amino acid residues, naturally occurring amino acid residues not encoded by a codon, non-naturally occurring amino acid residues.

[0087] As used herein "protein" refers to a series of amino acids, oligopeptide, peptide, polypeptide or portions thereof whether naturally occurring or synthetic.

[0088] As used herein a "recombinant polypeptide" is a polypeptide produced by translation of a recombinant polynucleotide.

[0089] A "synthetic polypeptide" is a polypeptide created by consecutive polymerization of isolated amino acid residues using methods known in the art.

[0090] An "isolated polypeptide", whether a naturally occurring or a recombinant polypeptide, is more enriched in (or out of) a cell than the polypeptide in its natural state in a wild-type cell, for example, more than about 5% enriched, more than about 10% enriched, or more than about 20%, or more than about 50%, or more, enriched, for example, alternatively denoted: 105%, 110%, 120%, 150% or more, enriched relative to wild type standardized at 100%. Such enrichment is not the result of a natural response of a wild-type plant. Alternatively, or additionally, the isolated polypeptide is separated from other cellular components, with which it is typically associated, for example, by any of the various protein purification methods.

[0091] As used herein, a "functional fragment" refers to a portion of a polypeptide provided herein which retains full or partial molecular, physiological or biochemical function of the full length polypeptide. A functional fragment often contains the domain(s), such as Pfam domains (see below), identified in the polypeptide provided in the sequence listing.

[0092] A "recombinant DNA construct" as used in the present disclosure comprises at least one expression cassette having a promoter operable in plant cells and a polynucleotide of the present disclosure. DNA constructs can be used as a means of delivering recombinant DNA constructs to a plant cell in order to effect stable integration of the recombinant molecule into the plant cell genome. In one embodiment, the polynucleotide can encode a protein or variant of a protein or fragment of a protein that is functionally defined to maintain activity in transgenic host cells including plant cells, plant parts, explants and whole plants. In another embodiment, the polynucleotide can encode a non-coding RNA that interferes with the functioning of endogenous classes of small RNAs that regulate expression, including but not limited to taRNAs, siRNAs and miRNAs. Recombinant DNA constructs are assembled using methods known to persons of ordinary skill in the art and typically comprise a promoter operably linked to DNA, the expression of which provides the enhanced agronomic trait.

[0093] Other construct components can include additional regulatory elements, such as 5' leaders and introns for enhancing transcription, 3' untranslated regions (such as polyadenylation signals and sites), and DNA for transit or targeting or signal peptides.

[0094] Percent identity describes the extent to which polynucleotides or protein segments are invariant in an alignment of sequences, for example nucleotide sequences or amino acid sequences. An alignment of sequences is created by manually aligning two sequences, for example, a stated sequence, as provided herein, as a reference, and another sequence, to produce the highest number of matching elements, for example, individual nucleotides or amino acids, while allowing for the introduction of gaps into either sequence. An "identity fraction" for a sequence aligned with a reference sequence is the number of matching elements, divided by the full length of the reference sequence, not including gaps introduced by the alignment process into the reference sequence. "Percent identity" ("% identity") as used herein is the identity fraction times 100.

[0095] As used herein, a "homolog" or "homologues" means a protein in a group of proteins that perform the same biological function, for example, proteins that belong to the same Pfam protein family and that provide a common enhanced trait in transgenic plants of this disclosure. Homologs are expressed by homologous genes. With reference to homologous genes, homologs include orthologs, for example, genes expressed in different species that evolved from common ancestral genes by speciation and encode proteins retain the same function, but do not include paralogs, i.e., genes that are related by duplication but have evolved to encode proteins with different functions. Homologous genes include naturally occurring alleles and artificially-created variants.

[0096] Degeneracy of the genetic code provides the possibility to substitute at least one base of the protein encoding sequence of a gene with a different base without causing the amino acid sequence of the polypeptide produced from the gene to be changed. When optimally aligned, homolog proteins, or their corresponding nucleotide sequences, have typically at least about 60% identity, in some instances at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or even at least about 99.5% identity over the full length of a protein or its corresponding nucleotide sequence identified as being associated with imparting an enhanced trait or altered phenotype when expressed in plant cells. In one aspect of the disclosure homolog proteins have at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or at least about 99.5% identity to a consensus amino acid sequence of proteins and homologs that can be built from sequences disclosed herein.

[0097] Homologs are inferred from sequence similarity, by comparison of protein sequences, for example, manually or by use of a computer-based tool using known sequence comparison algorithms such as BLAST and FASTA. A sequence search and local alignment program, for example, BLAST, can be used to search query protein sequences of a base organism against a database of protein sequences of various organisms, to find similar sequences, and the summary Expectation value (E-value) can be used to measure the level of sequence similarity. Because a protein hit with the lowest E-value for a particular organism may not necessarily be an ortholog or be the only ortholog, a reciprocal query is used to filter hit sequences with significant E-values for ortholog identification. The reciprocal query entails search of the significant hits against a database of protein sequences of the base organism. A hit can be identified as an ortholog, when the reciprocal query's best hit is the query protein itself or a paralog of the query protein. With the reciprocal query process orthologs are further differentiated from paralogs among all the homologs, which allows for the inference of functional equivalence of genes. A further aspect of the homologs encoded by DNA useful in the transgenic plants of the invention are those proteins that differ from a disclosed protein as the result of deletion or insertion of one or more amino acids in a native sequence.

[0098] Other functional homolog proteins differ in one or more amino acids from those of a trait-improving protein disclosed herein as the result of one or more of known conservative amino acid substitutions, for example, valine is a conservative substitute for alanine and threonine is a conservative substitute for serine. Conservative substitutions for an amino acid within the native sequence can be selected from other members of a class to which the naturally occurring amino acid belongs. Representative amino acids within these various classes include, but are not limited to: (1) acidic (negatively charged) amino acids such as aspartic acid and glutamic acid; (2) basic (positively charged) amino acids such as arginine, histidine, and lysine; (3) neutral polar amino acids such as glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; and (4) neutral nonpolar (hydrophobic) amino acids such as alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine. Conserved substitutes for an amino acid within a native protein or polypeptide can be selected from other members of the group to which the naturally occurring amino acid belongs. For example, a group of amino acids having aliphatic side chains is glycine, alanine, valine, leucine, and isoleucine; a group of amino acids having aliphatic-hydroxyl side chains is serine and threonine; a group of amino acids having amide-containing side chains is asparagine and glutamine; a group of amino acids having aromatic side chains is phenylalanine, tyrosine, and tryptophan; a group of amino acids having basic side chains is lysine, arginine, and histidine; and a group of amino acids having sulfur-containing side 30 chains is cysteine and methionine. Naturally conservative amino acids substitution groups are: valine-leucine, valine-isoleucine, phenylalanine-tyrosine, lysine-arginine, alaninevaline, aspartic acid-glutamic acid, and asparagine-glutamine A further aspect of the disclosure includes proteins that differ in one or more amino acids from those of a described protein sequence as the result of deletion or insertion of one or more amino acids in a native sequence.

[0099] In general, the term "variant" refers to molecules with some differences, generated synthetically or naturally, in their nucleotide or amino acid sequences as compared to a reference (native) polynucleotides or polypeptides, respectively. These differences include substitutions, insertions, deletions or any desired combinations of such changes in a native polynucleotide or amino acid sequence.

[0100] With regard to polynucleotide variants, differences between presently disclosed polynucleotides and polynucleotide variants are limited so that the nucleotide sequences of the former and the latter are similar overall and, in many regions, identical. Due to the degeneracy of the genetic code, differences between the former and the latter nucleotide sequences may be silent (for example, the amino acids encoded by the polynucleotide are the same, and the variant polynucleotide sequence encodes the same amino acid sequence as the presently disclosed polynucleotide). Variant nucleotide sequences can encode different amino acid sequences, in which case such nucleotide differences will result in amino acid substitutions, additions, deletions, insertions, truncations or fusions with respect to the similarly disclosed polynucleotide sequences. These variations can result in polynucleotide variants encoding polypeptides that share at least one functional characteristic. The degeneracy of the genetic code also dictates that many different variant polynucleotides can encode identical and/or substantially similar polypeptides.

[0101] As used herein "gene" or "gene sequence" refers to the partial or complete coding sequence of a gene, its complement, and its 5' and/or 3' untranslated regions (UTRs) and their complements. A gene is also a functional unit of inheritance, and in physical terms is a particular segment or sequence of nucleotides along a molecule of DNA (or RNA, in the case of RNA viruses) involved in producing a polypeptide chain. The latter can be subjected to subsequent processing such as chemical modification or folding to obtain a functional protein or polypeptide. By way of example, a transcriptional regulator gene encodes a transcriptional regulator polypeptide, which can be functional or require processing to function as an initiator of transcription.

[0102] As used herein, the term "promoter" refers generally to a DNA molecule that is involved in recognition and binding of RNA polymerase II and other proteins (trans-acting transcription factors) to initiate transcription. A promoter can be initially isolated from the 5' untranslated region (5' UTR) of a genomic copy of a gene. Alternately, promoters can be synthetically produced or manipulated or engineered DNA molecules. Promoters can also be chimeric, that is a promoter produced through the fusion of two or more heterologous DNA molecules. Plant promoters include promoter DNA obtained from plants, plant viruses, fungi and bacteria such as Agrobacterium and Bradyrhizobium bacteria. A promoter may also be heterologous. As used herein, a promoter or other regulatory sequence operably linked to a transcribable DNA sequence, such as a coding sequence, is considered "heterologous" if in nature the promoter or regulatory sequence is not operably linked to the transcribable DNA sequence and/or is not present in the plant host cell to be transformed with the promoter or regulatory sequence. Two or more promoter or regulatory sequences may also be heterologous with respect to each other.

[0103] Promoters which initiate transcription in all or most tissues of the plant are referred to as "constitutive" promoters. Promoters which initiate transcription during certain periods or stages of development are referred to as "developmental" promoters. Promoters whose expression is enhanced in certain tissues of the plant relative to other plant tissues are referred to as "tissue enhanced" or "tissue preferred" promoters. Promoters which express within a specific tissue of the plant, with little or no expression in other plant tissues are referred to as "tissue specific" promoters. A promoter that expresses in a certain cell type of the plant, for example a microspore mother cell, is referred to as a "cell type specific" promoter. An "inducible" promoter is a promoter in which transcription is initiated in response to an environmental stimulus such as cold, drought or light; or other stimuli such as wounding or chemical application. Many physiological and biochemical processes in plants exhibit endogenous rhythms with a period of about 24 hours. A "diurnal promoter" is a promoter which exhibits altered expression profiles under the control of a circadian oscillator. Diurnal regulation is subject to environmental inputs such as light and temperature and coordination by the circadian clock. Many examples of plant expressible promoters are known in the art.

[0104] Sufficient expression in plant seed tissues is desired to affect improvements in seed composition. Exemplary promoters for use for seed composition modification include promoters from seed genes such as napin as disclosed in U.S. Pat. No. 5,420,034, maize L3 oleosin as disclosed in U.S. Pat. No. 6,433,252, zein Z27 as disclosed by Russell et al. (1997) Transgenic Res. 6(2):157-166, globulin 1 as disclosed by Belanger et al (1991) Genetics 129:863-872, glutelin 1 as disclosed by Russell (1997) supra, and peroxiredoxin antioxidant (Per1) as disclosed by Stacy et al. (1996) Plant Mol Biol. 31(6):1205-1216.

[0105] As used herein, the term "leader" refers to a DNA molecule isolated from the untranslated 5' region (5' UTR) of a genomic copy of a gene and is defined generally as a nucleotide segment between the transcription start site (TSS) and the protein coding sequence start site. Alternately, leaders can be synthetically produced or manipulated DNA elements. A leader can be used as a 5' regulatory element for modulating expression of an operably linked transcribable polynucleotide molecule. As used herein, the term "intron" refers to a DNA molecule that can be isolated or identified from the genomic copy of a gene and can be defined generally as a region spliced out during mRNA processing prior to translation. Alternately, an intron can be a synthetically produced or manipulated DNA element. An intron can contain enhancer elements that effect the transcription of operably linked genes. An intron can be used as a regulatory element for modulating expression of an operably linked transcribable polynucleotide molecule. A DNA construct can comprise an intron, and the intron may or may not be with respect to the transcribable polynucleotide molecule.

[0106] As used herein, the term "enhancer" or "enhancer element" refers to a cis-acting transcriptional regulatory element, a.k.a. cis-element, which confers an aspect of the overall expression pattern, but is usually insufficient alone to drive transcription, of an operably linked polynucleotide. Unlike promoters, enhancer elements do not usually include a transcription start site (TSS) or TATA box or equivalent sequence. A promoter can naturally comprise one or more enhancer elements that affect the transcription of an operably linked polynucleotide. An isolated enhancer element can also be fused to a promoter to produce a chimeric promoter cis-element, which confers an aspect of the overall modulation of gene expression. A promoter or promoter fragment can comprise one or more enhancer elements that effect the transcription of operably linked genes. Many promoter enhancer elements are believed to bind DNA-binding proteins and/or affect DNA topology, producing local conformations that selectively allow or restrict access of RNA polymerase to the DNA template or that facilitate selective opening of the double helix at the site of transcriptional initiation. An enhancer element can function to bind transcription factors that regulate transcription. Some enhancer elements bind more than one transcription factor, and transcription factors can interact with different affinities with more than one enhancer domain.

[0107] Expression cassettes of this disclosure can include a "transit peptide" or "targeting peptide" or "signal peptide" molecule located either 5' or 3' to or within the gene(s). These terms generally refer to peptide molecules that when linked to a protein of interest directs the protein to a particular tissue, cell, subcellular location, or cell organelle. Examples include, but are not limited to, chloroplast transit peptides (CTPs), chloroplast targeting peptides, mitochondrial targeting peptides, nuclear targeting signals, nuclear exporting signals, vacuolar targeting peptides, and vacuolar sorting peptides. For description of the use of chloroplast transit peptides see U.S. Pat. No. 5,188,642 and U.S. Pat. No. 5,728,925. For description of the transit peptide region of an Arabidopsis EPSPS gene in the present disclosure, see Klee, H. J. Et al (MGG (1987) 210:437-442. Expression cassettes of this disclosure can also include an intron or introns. Expression cassettes of this disclosure can contain a DNA near the 3' end of the cassette that acts as a signal to terminate transcription from a heterologous nucleic acid and that directs polyadenylation of the resultant mRNA. These are commonly referred to as "3'-untranslated regions" or "3'-non-coding sequences" or "3'-UTRs". The "3' non-translated sequences" means DNA sequences located downstream of a structural nucleotide sequence and include sequences encoding polyadenylation and other regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal functions in plants to cause the addition of polyadenylate nucleotides to the 3' end of the mRNA precursor. The polyadenylation signal can be derived from a natural gene, from a variety of plant genes, or from T-DNA. An example of a polyadenylation sequence is the nopaline synthase 3' sequence (nos 3'; Fraley et al., Proc. Natl. Acad. Sci. USA 80: 4803-4807, 1983). The use of different 3' non-translated sequences is exemplified by Ingelbrecht et al., Plant Cell 1:671-680, 1989.

[0108] Expression cassettes of this disclosure can also contain one or more genes that encode selectable markers and confer resistance to a selective agent such as an antibiotic or an herbicide. A number of selectable marker genes are known in the art and can be used in the present disclosure: selectable marker genes conferring tolerance to antibiotics like kanamycin and paromomycin (nptII), hygromycin B (aph IV), spectinomycin (aadA), U.S. Patent Publication 2009/0138985A1 and gentamycin (aac3 and aacC4) or tolerance to herbicides like glyphosate (for example, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), U.S. Pat. No. 5,627,061; U.S. Pat. No. 5,633,435; U.S. Pat. No. 6,040,497; U.S. Pat. No. 5,094,945), sulfonyl herbicides (for example, acetohydroxyacid synthase or acetolactate synthase conferring tolerance to acetolactate synthase inhibitors such as sulfonylurea, imidazolinone, triazolopyrimidine, pyrimidyloxybenzoates and phthalide (U.S. Pat. Nos. 6,225,105; 5,767,366; 4,761,373; 5,633,437; 6,613,963; 5,013,659; 5,141,870; 5,378,824; 5,605,011)), bialaphos or phosphinothricin or derivatives (e.g., phosphinothricin acetyltransferase (bar) tolerance to phosphinothricin or glufosinate (U.S. Pat. Nos. 5,646,024; 5,561,236; 5,276,268; 5,637,489; 5,273,894); dicamba (dicamba monooxygenase, Patent Application Publications US2003/0115626A1), or sethoxydim (modified acetyl-coenzyme A carboxylase for conferring tolerance to cyclohexanedione), and aryloxyphenoxypropionate (haloxyfop, U.S. Pat. No. 6,414,222).

[0109] Transformation vectors of this disclosure can contain one or more "expression cassettes", each comprising a native or non-native plant promoter operably linked to a polynucleotide sequence of interest, which is operably linked to a 3' UTR sequence and termination signal, for expression in an appropriate host cell. It also typically comprises sequences required for proper translation of the polynucleotide or transgene. As used herein, the term "transgene" refers to a polynucleotide molecule artificially incorporated into a host cell's genome. Such a transgene can be heterologous to the host cell. The term "transgenic plant" refers to a plant comprising such a transgene. The coding region usually codes for a protein of interest but can also code for a functional RNA of interest, for example an antisense RNA, a nontranslated RNA, in the sense or antisense direction, a miRNA, a noncoding RNA, or a synthetic RNA used in either suppression or over expression of target gene sequences. The expression cassette comprising the nucleotide sequence of interest can be chimeric, meaning that at least one of its components is heterologous with respect to at least one of its other components. As used herein the term "chimeric" refers to a DNA molecule that is created from two or more genetically diverse sources, for example a first molecule from one gene or organism and a second molecule from another gene or organism.

[0110] Recombinant DNA constructs in this disclosure generally include a 3' element that typically contains a polyadenylation signal and site. Known 3' elements include those from Agrobacterium tumefaciens genes such as nos 3', tml 3', tmr 3', tms 3', ocs 3', tr7 3', for example disclosed in U.S. Pat. No. 6,090,627; 3' elements from plant genes such as wheat (Triticum aesevitum) heat shock protein 17 (Hsp17 3'), a wheat ubiquitin gene, a wheat fructose-1,6-biphosphatase gene, a rice glutelin gene, a rice lactate dehydrogenase gene and a rice beta-tubulin gene, all of which are disclosed in U.S. Patent Application Publication 2002/0192813 A1; and the pea (Pisum sativum) ribulose biphosphate carboxylase gene (rbs 3'), and 3' elements from the genes within the host plant.

[0111] As an alternative to traditional transformation methods, a DNA sequence, such as a transgene, expression cassette(s), etc., may be inserted or integrated into a specific site or locus within the genome of a plant or plant cell via site-directed integration. Recombinant DNA construct(s) and molecule(s) of this disclosure may thus include a donor template sequence comprising at least one transgene, expression cassette, or other DNA sequence for insertion into the genome of the plant or plant cell. Such donor template for site-directed integration may further include one or two homology arms flanking an insertion sequence (i.e., the sequence, transgene, cassette, etc., to be inserted into the plant genome). The recombinant DNA construct(s) of this disclosure may further comprise an expression cassette(s) encoding a site-specific nuclease and/or any associated protein(s) to carry out site-directed integration. These nuclease expressing cassette(s) may be present in the same molecule or vector as the donor template (in cis) or on a separate molecule or vector (in trans). Several methods for site-directed integration are known in the art involving different proteins (or complexes of proteins and/or guide RNA) that cut the genomic DNA to produce a double strand break (DSB) or nick at a desired genomic site or locus. Briefly as understood in the art, during the process of repairing the DSB or nick introduced by the nuclease enzyme, the donor template DNA may become integrated into the genome at the site of the DSB or nick. The presence of the homology arm(s) in the donor template may promote the adoption and targeting of the insertion sequence into the plant genome during the repair process through homologous recombination, although an insertion event may occur through non-homologous end joining (NHEJ). Examples of site-specific nucleases that may be used include zinc-finger nucleases, engineered or native meganucleases, TALE-endonucleases, and RNA-guided endonucleases (e.g., Cas9 or Cpf1). For methods using RNA-guided site-specific nucleases (e.g., Cas9 or Cpf1), the recombinant DNA construct(s) will also comprise a sequence encoding one or more guide RNAs to direct the nuclease to the desired site within the plant genome.

[0112] As used herein, the term "homology arm" refers to a polynucleotide sequence that has at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to a target sequence in a plant or plant cell that is being transformed. A homology arm can comprise at least 15, at least 20, at least 25, at least 30, at least 40, at least 50, at least 100, at least 250, at least 500, or at least 1000 nucleotides.

[0113] As used herein "operably linked" means the association of two or more DNA fragments in a recombinant DNA construct so that the function of one, for example, protein-encoding DNA, is controlled by the other, for example, a promoter.

[0114] Transgenic plants can comprise a stack of one or more polynucleotides disclosed herein resulting in the production of multiple polypeptide sequences. Transgenic plants comprising stacks of polynucleotides can be obtained by either or both of traditional breeding methods or through genetic engineering methods. These methods include, but are not limited to, crossing individual transgenic lines each comprising a polynucleotide of interest, transforming a transgenic plant comprising a first gene disclosed herein with a second gene, and co-transformation of genes into a single plant cell. Co-transformation of genes can be carried out using single transformation vectors comprising multiple genes or genes carried separately on multiple vectors.

[0115] Transgenic plants comprising or derived from plant cells of this disclosure transformed with recombinant DNA can be further enhanced with stacked traits, for example, a crop plant having an enhanced trait resulting from expression of DNA disclosed herein in combination with herbicide and/or pest resistance traits. For example, genes of the current disclosure can be stacked with other traits of agronomic interest, such as a trait providing herbicide resistance, or insect resistance, such as using a gene from Bacillus thuringensis to provide resistance against lepidopteran, coliopteran, homopteran, hemiopteran, and other insects, or improved quality traits such as improved nutritional value. Herbicides for which transgenic plant tolerance has been demonstrated and the method of the present disclosure can be applied include, but are not limited to, glyphosate, dicamba, glufosinate, sulfonylurea, bromoxynil, norflurazon, 2,4-D (2,4-dichlorophenoxy) acetic acid, aryloxyphenoxy propionates, p-hydroxyphenyl pyruvate dioxygenase inhibitors (HPPD), and protoporphyrinogen oxidase inhibitors (PPO) herbicides. Polynucleotide molecules encoding proteins involved in herbicide tolerance known in the art and include, but are not limited to, a polynucleotide molecule encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) disclosed in U.S. Pat. Nos. 5,094,945; 5,627,061; 5,633,435 and 6,040,497 for imparting glyphosate tolerance; polynucleotide molecules encoding a glyphosate oxidoreductase (GOX) disclosed in U.S. Pat. No. 5,463,175 and a glyphosate-N-acetyl transferase (GAT) disclosed in U.S. Patent No. Application Publication 2003/0083480 A1 also for imparting glyphosate tolerance; dicamba monooxygenase disclosed in U.S. Patent Application Publication 2003/0135879 A1 for imparting dicamba tolerance; a polynucleotide molecule encoding bromoxynil nitrilase (Bxn) disclosed in U.S. Pat. No. 4,810,648 for imparting bromoxynil tolerance; a polynucleotide molecule encoding phytoene desaturase (crtl) described in Misawa et al, (1993) Plant J. 4:833-840 and in Misawa et al, (1994) Plant J. 6:481-489 for norflurazon tolerance; a polynucleotide molecule encoding acetohydroxyacid synthase (AHAS, aka ALS) described in Sathasiivan et al. (1990) Nucl. Acids Res. 18:2188-2193 for imparting tolerance to sulfonylurea herbicides; polynucleotide molecules known as bar genes disclosed in DeBlock, et al. (1987) EMBO J. 6:2513-2519 for imparting glufosinate and bialaphos tolerance; polynucleotide molecules disclosed in U.S. Patent Application Publication 2003/010609 A1 for imparting N-amino methyl phosphonic acid tolerance; polynucleotide molecules disclosed in U.S. Pat. No. 6,107,549 for imparting pyridine herbicide resistance; molecules and methods for imparting tolerance to multiple herbicides such as glyphosate, atrazine, ALS inhibitors, isoxoflutole and glufosinate herbicides are disclosed in U.S. Pat. No. 6,376,754 and U.S. Patent Application Publication 2002/0112260. Molecules and methods for imparting insect/nematode/virus resistance are disclosed in U.S. Pat. Nos. 5,250,515; 5,880,275; 6,506,599; 5,986,175 and U.S. Patent Application Publication 2003/0150017 A1.

Plant Cell Transformation Methods

[0116] Numerous methods for transforming chromosomes and plastids in a plant cell with recombinant DNA are known in the art that may be used in methods of producing a transgenic plant cell and plant. Two effective methods for transformation are Agrobacterium-mediated transformation and microprojectile bombardment-mediated transformation. Microprojectile bombardment methods are illustrated, for example, in U.S. Pat. Nos. 5,015,580 (soybean); 5,550,318 (corn); 5,538,880 (corn); 5,914,451 (soybean); 6,160,208 (corn); 6,399,861 (corn); 6,153,812 (wheat) and 6,365,807 (rice). Agrobacterium-mediated transformation methods are described, for example, in U.S. Patents 5,159,135 (cotton); 5,824,877 (soybean); 5,463,174 (canola); 5,591,616 (corn); 5,846,797 (cotton); 8,044,260 (cotton); 6,384,301 (soybean), 7,026,528 (wheat) and 6,329,571 (rice), U.S. Patent Application Publication No. 2004/0087030 A1 (cotton), and U.S. Patent Application Publication No. 2001/0042257 A1 (sugar beet), all of which are incorporated herein by reference in their entirety. Transformation of plant material is practiced in tissue culture on nutrient media, for example a mixture of nutrients that allow cells to grow in vitro. Recipient cell targets include, but are not limited to, meristem cells, shoot tips, hypocotyls, calli, immature or mature embryos, and gametic cells such as microspores, pollen, sperm and egg cells. Callus can be initiated from tissue sources including, but not limited to, immature or mature embryos, hypocotyls, seedling apical meristems, microspores and the like. Cells containing a transgenic nucleus are grown into transgenic plants.

[0117] As introduced above, another method for transforming chromosomes in a plant cell is via insertion of a DNA sequence using a recombinant DNA donor template at a pre-determined site of the genome by methods of site-directed integration. Site-directed integration may be accomplished by any method known in the art, for example, by use of zinc-finger nucleases, engineered or native meganucleases, TALE-endonucleases, or an RNA-guided endonuclease (for example Cas9 or Cpf1). The recombinant DNA construct may be inserted at the pre-determined site by homologous recombination (HR) or by non-homologous end joining (NHEJ). In addition to insertion of a recombinant DNA construct into a plant chromosome at a pre-determined site, genome editing can be achieved through oligonucleotide-directed mutagenesis (ODM) (Oh and May, 2001; U.S. Pat. No. 8,268,622) or by introduction of a double-strand break (DSB) or nick with a site specific nuclease, followed by NHEJ or repair. The repair of the DSB or nick may be used to introduce insertions or deletions at the site of the DSB or nick, and these mutations may result in the introduction of frame-shifts, amino acid substitutions, and/or an early termination codon of protein translation or alteration of a regulatory sequence of a gene. Genome editing may be achieved with or without a donor template molecule.

[0118] In addition to direct transformation of a plant material with a recombinant DNA construct, a transgenic plant can be prepared by crossing a first plant comprising a recombinant DNA with a second plant lacking the recombinant DNA. For example, recombinant DNA can be introduced into a first plant line that is amenable to transformation, which can be crossed with a second plant line to introgress the recombinant DNA into the second plant line. A transgenic plant with recombinant DNA providing an enhanced trait, for example, enhanced yield, can be crossed with a transgenic plant line having other recombinant DNA that confers another trait, for example herbicide resistance or pest resistance, to produce progeny plants having recombinant DNA that confers both traits. Typically, in such breeding for combining traits the transgenic plant donating the additional trait is a male line and the transgenic plant carrying the base traits is the female line. The progeny of this cross will segregate such that some of the plants will carry the DNA for both parental traits and some will carry DNA for one parental trait; such plants can be identified by markers associated with parental recombinant DNA, for example, marker identification by analysis for recombinant DNA or, in the case where a selectable marker is linked to the recombinant, by application of the selecting agent such as a herbicide for use with a herbicide tolerance marker, or by selection for the enhanced trait. Progeny plants carrying DNA for both parental traits can be crossed back into the female parent line multiple times, for example usually 6 to 8 generations, to produce a progeny plant with substantially the same genotype as the original transgenic parental line but for the recombinant DNA of the other transgenic parental line.

[0119] For transformation, DNA is typically introduced into only a small percentage of target plant cells in any one transformation experiment. Marker genes are used to provide an efficient system for identification of those cells that are stably transformed by receiving and integrating a recombinant DNA construct into their genomes. Preferred marker genes provide selective markers which confer resistance to a selective agent, such as an antibiotic or an herbicide. Any of the herbicides to which plants of this disclosure can be resistant is an agent for selective markers. Potentially transformed cells are exposed to the selective agent. In the population of surviving cells are those cells where, generally, the resistance-conferring gene is integrated and expressed at sufficient levels to permit cell survival. Cells can be tested further to confirm stable integration of the exogenous DNA. Commonly used selective marker genes include those conferring resistance to antibiotics such as kanamycin and paromomycin (nptII), hygromycin B (aph IV), spectinomycin (aadA) and gentamycin (aac3 and aacC4) or resistance to herbicides such as glufosinate (bar or pat), dicamba (DMO) and glyphosate (aroA or EPSPS). Examples of such selectable markers are illustrated in U.S. Pat. Nos. 5,550,318; 5,633,435; 5,780,708; 6,118,047 and 8,030,544. Markers which provide an ability to visually screen transformants can also be employed, for example, a gene expressing a colored or fluorescent protein such as a luciferase or green fluorescent protein (GFP) or a gene expressing a beta-glucuronidase or uidA gene (GUS) for which various chromogenic substrates are known.

[0120] Plant cells that survive exposure to a selective agent, or plant cells that have been scored positive in a screening assay, may be cultured in vitro to develop or regenerate plantlets. Developing plantlets regenerated from transformed plant cells can be transferred to plant growth mix, and hardened off, for example, in an environmentally controlled chamber at about 85% relative humidity, 600 ppm CO.sub.2, and 25-250 microeinsteins m .sup.2 s.sup.-1 of light, prior to transfer to a greenhouse or growth chamber for maturation. Plants may be regenerated from about 6 weeks to 10 months after a transformant is identified, depending on the initial tissue, and plant species. Plants can be pollinated using conventional plant breeding methods known to those of skill in the art to produce seeds, for example self-pollination is commonly used with transgenic corn. The regenerated transformed plant or its progeny seed or plants can be tested for expression of the recombinant DNA and selected for the presence of an altered phenotype or an enhanced agronomic trait.

Transgenic Plants and Seeds

[0121] Transgenic plants derived from transgenic plant cells having a transgenic nucleus of this disclosure are grown to generate transgenic plants having an altered phenotype or an enhanced trait as compared to a control plant, and produce transgenic seed and haploid pollen of this disclosure. Such plants with enhanced traits are identified by selection of transformed plants or progeny seed for the enhanced trait. For efficiency a selection method is designed to evaluate multiple transgenic plants (events) comprising the recombinant DNA, for example multiple plants from 2 to 20 or more transgenic events. Transgenic plants grown from transgenic seeds provided herein demonstrate improved agronomic traits that contribute to increased yield or other traits that provide increased plant value, including, for example, improved seed quality. Of particular interest are plants having increased water use efficiency or drought tolerance, enhanced high temperature or cold tolerance, increased yield, and increased nitrogen use efficiency.

[0122] Table 1 provides a list of sequences of protein-encoding genes as recombinant DNA for production of transgenic plants with enhanced traits. The elements of Table 1 are described by reference to: "NUC SEQ ID NO." which identifies a DNA sequence; "PEP SEQ ID NO." which identifies an amino acid sequence; "Gene ID" which refers to an arbitrary identifier; and "Gene Name and Description" which is a common name and functional description of the gene.

TABLE-US-00001 TABLE 1 Sequences for Protein-Coding Genes NUC PEP SEQ ID SEQ ID NO. NO. Gene ID Gene Name and Description 1 30 T5MON01 Agrobacterium ornithine carbamoyl transferase (argF) 2 31 T5MON02 Arabidopsis MADS-box protein (AGL20) 3 32 T5MON03 Arabidopsis containing Pleckstrin homology domain, 6 regulator of chromosome condensation (RCC1) domains, and Zinc finger domain 4 33 T5MON04 Arabidopsis Glycyl-tRNA synthetase 5 34 T5MON05 Arabidopsis L-ascorbate oxidase 6 35 T5MON06 Arabidopsis glutamate decarboxylase 4 (GAD4) 7 36 T5MON07 Arabidopsis growth factor like protein 8 37 T5MON08 Arabidopsis Aromatic and neutral amino acid transporter 1-like (ANT1-like) 9 38 T5MON09 Arabidopsis putative AP2/EREBP transcription factor (with A33V mutation) 10 39 T5MON10 Arabidopsis dark inducible 11 (DIN11) 11 40 T5MON11 Arabidopsis strictosidine synthase family protein, mucin-like (AtSSL7) 12 41 T5MON12 Arabidopsis purple acid phosphatase precursor (AtPAP) 13 42 T5MON13 Arabidopsis serine/threonine protein kinase, root hair specific 3 (RHS3) 14 43 T5MON14 Chlamydomonas reinhardtii PHE0023587_Ferredoxin-dependent glutamate synthase (GSF1) 15 44 T5MON15 Chlorella sorokiniana NADP-specific glutamate dehydrogenase (NADP-GDH), N terminus residues 1 to 74 truncated 16 45 T5MON16 E coli glutamate dehydrogenase, NADP-specific (gdhA) 17 46 T5MON17 Homeobox fusion protein, Arabidopsis HB-17 N terminus residues 1 to 91 fused to soybean HB-17 C terminus residues 20-213 18 47 T5MON18 Soybean AP2-EREBP transcription factor (ERF3) 19 48 T5MON19 rice Ghd7 20 49 T5MON20 Corn pyruvate orthophosphate dikinase 1 (PPDK1) 21 50 T5MON21 Corn proliferating cell nuclear antigen (PCNA2) 22 51 T5MON23 Corn Roothairless 1 (RTH1) 23 52 T5MON24 Corn orphans transcription factor (Orphan46) 24 53 T5MON25 Corn MADS transcription factor (MADS12) 25 54 T5MON26 Corn remorin like DNA-binding protein 2 26 55 T5MON27 Corn CAAT-box DNA binding protein (NFBa) 27 56 T5MON28 Corn homeobox-leucine zipper protien, HB transcription factor 28 57 T5MON29 Corn putative glutamine synthetase (with C299A mutation) 29 58 T5MON30 Corn putative nucleostemin

Selecting and Testing Transgenic Plants for Enhanced Traits

[0123] Within a population of transgenic plants each developed or regenerated from a plant cell with recombinant DNA many plants that survive to fertile transgenic plants that produce seeds and progeny plants will not exhibit an enhanced agronomic trait. Selection from the population is necessary to identify one or more transgenic plants with an enhanced trait. Further evaluation with vigorous testing is essential for understanding the contributing components to a trait, supporting trait advancement decisions and generating mode of action hypotheses. Transgenic plants having enhanced traits are selected and tested from populations of plants developed, regenerated or derived from plant cells transformed as described herein by evaluating the plants in a variety of assays to detect an enhanced trait, for example, increased water use efficiency or drought tolerance, enhanced high temperature or cold tolerance, increased yield or yield components, desirable architecture, optimum life cycle, increased nitrogen use efficiency, enhanced seed composition such as enhanced seed protein and enhanced seed oil.

[0124] These assays can take many forms including, but not limited to, direct screening for the trait in a greenhouse or field trial or by screening for a surrogate trait. Such analyses can be directed to detecting changes in the chemical composition, biomass, yield components, physiological property, root architecture, morphology, or life cycle of the plant. Changes in chemical compositions such as nutritional composition of grain can be detected by analysis of the seed composition and content of protein, free amino acids, oil, free fatty acids, starch or tocopherols. Changes in chemical compositions can also be detected by analysis of contents in leaves, such as chlorophyll or carotenoid contents. Changes in biomass characteristics can be evaluated on greenhouse or field grown plants and can include plant height, stem diameter, root and shoot dry weights, canopy size; and, for corn plants, ear length and diameter. Changes in yield components can be measured by total number of kernels per unit area and its individual weight. Changes in physiological properties can be identified by evaluating responses to stress conditions, for example assays using imposed stress conditions such as water deficit, nitrogen deficiency, cold growing conditions, pathogen or insect attack or light deficiency, or increased plant density. Changes in root architecture can be evaluated by root length and branch number. Changes in morphology can be measured by visual observation of tendency of a transformed plant to appear to be a normal plant as compared to changes toward bushy, taller, thicker, narrower leaves, striped leaves, knotted trait, chlorosis, albino, anthocyanin production, or altered tassels, ears or roots. Changes in morphology can also be measured with morphometric analysis based on shape parameters, using dimensional measurement such as ear diameter, ear length, kernel row number, internode length, plant height, or stem volume. Changes in life cycle can be measured by macro or microscopic morphological changes partitioned into developmental stages, such as days to pollen shed, days to silking, leaf extension rate. Other selection and testing properties include days to pollen shed, days to silking, leaf extension rate, chlorophyll content, leaf temperature, stand, seedling vigor, internode length, plant height, leaf number, leaf area, tillering, brace roots, stay green or delayed senescence, stalk lodging, root lodging, plant health, bareness/prolificacy, green snap, and pest resistance. In addition, phenotypic characteristics of harvested grain can be evaluated, including number of kernels per row on the ear, number of rows of kernels on the ear, kernel abortion, kernel weight, kernel size, kernel density and physical grain quality.

[0125] Assays for screening for a desired trait are readily designed by those practicing in the art. The following illustrates screening assays for corn traits using hybrid corn plants. The assays can be adapted for screening other plants such as canola, wheat, cotton and soybean either as hybrids or inbreds.

[0126] Transgenic corn plants having increased nitrogen use efficiency can be identified by screening transgenic plants in the field under the same and sufficient amount of nitrogen supply as compared to control plants, where such plants provide higher yield as compared to control plants. Transgenic corn plants having increased nitrogen use efficiency can also be identified by screening transgenic plants in the field under reduced amount of nitrogen supply as compared to control plants, where such plants provide the same or similar yield as compared to control plants.

[0127] Transgenic corn plants having increased yield are identified by screening using progenies of the transgenic plants over multiple locations for several years with plants grown under optimal production management practices and maximum weed and pest control or standard agronomic practices (SAP). Selection methods can be applied in multiple and diverse geographic locations, for example up to 16 or more locations, over one or more planting seasons, for example at least two planting seasons, to statistically distinguish yield improvement from natural environmental effects.

[0128] Transgenic corn plants having increased water use efficiency or drought tolerance are identified by screening plants in an assay where water is withheld for a period to induce stress followed by watering to revive the plants. For example, a selection process imposes 3 drought/re-water cycles on plants over a total period of 15 days after an initial stress free growth period of 11 days. Each cycle consists of 5 days, with no water being applied for the first four days and a water quenching on the 5th day of the cycle. The primary phenotypes analyzed by the selection method are the changes in plant growth rate as determined by height and biomass during a vegetative drought treatment.

[0129] Although the plant cells and methods of this disclosure can be applied to any plant cell, plant, seed or pollen, for example, any fruit, vegetable, grass, tree or ornamental plant, the various aspects of the disclosure are applied to corn, soybean, cotton, canola, rice, barley, oat, wheat, turf grass, alfalfa, sugar beet, sunflower, quinoa and sugar cane plants.

EXAMPLES

Example 1

Corn Transformation

[0130] This example illustrates transformation methods to produce a transgenic corn plant cell, seed, and plant having altered phenotypes as shown in Tables 3-5, and enhanced traits, increased water use efficiency, increased nitrogen use efficiency, and increased yield and altered traits and phenology as shown in Tables 7, 8 and 10.

[0131] For Agrobacterium-mediated transformation of corn embryo cells, ears from corn plants were harvested and surface-sterilized by spraying or soaking the ears in ethanol, followed by air drying. Embryos were isolated from individual kernels of surface-sterilized ears. After excision, maize embryos were inoculated with Agrobacterium cells containing plasmid DNA with the gene of interest cassette and a plant selectable marker cassette, and then co-cultured with Agrobacterium for several days. Co-cultured embryos were transferred to various selection and regeneration media, and transformed RO plants were recovered 6 to 8 weeks after initiation of selection, which were transplanted into potting soil. Regenerated RO plants were selfed, and R1 and subsequent progeny generations were obtained.

[0132] The above process can be repeated to produce multiple events of transgenic corn plants from cells that were transformed with recombinant DNA having the genes identified in Table 1. Progeny transgenic plants and seeds of the transformed plants were screened for the presence and single copy of the inserted gene, and for various altered or enhanced traits and phenotypes, such as increased water use efficiency, increased yield, and increased nitrogen use efficiency as shown in Tables 3-5, 7, 8 and 10. From each group of multiple events of transgenic plants with a specific recombinant DNA from Table 1, the event(s) that showed increased yield, increased water use efficiency, increased nitrogen use efficiency, and altered phenotypes and traits were identified.

Example 2

Soybean Transformation

[0133] This example illustrates plant transformation in producing a transgenic soybean plant cell, seed, and plant having an altered phenotype or an enhanced trait, such as increased water use efficiency, drought tolerance and increased yield as shown in Tables 9 and 10.

[0134] For Agrobacterium mediated transformation, soybean seeds were imbibed overnight and the meristem explants excised. Soybean explants were mixed with induced Agrobacterium cells containing plasmid DNA with the gene of interest cassette and a plant selectable marker cassette no later than 14 hours from the time of initiation of seed imbibition, and wounded using sonication. Following wounding, explants were placed in co-culture for 2-5 days at which point they were transferred to selection media to allow selection and growth of transgenic shoots. Resistant shoots were harvested in approximately 6-8 weeks and placed into selective rooting media for 2-3 weeks. Shoots producing roots were transferred to the greenhouse and potted in soil. Shoots that remained healthy on selection, but did not produce roots were transferred to non-selective rooting media for an additional two weeks. Roots from any shoots that produced roots off selection were tested for expression of the plant selectable marker before they were transferred to the greenhouse and potted in soil.

[0135] The above process can be repeated to produce multiple events of transgenic soybean plants from cells that were transformed with recombinant DNA having the genes identified in Table 1. Progeny transgenic plants and seed of the transformed plants were screened for the presence and single copy of the inserted gene, and tested for various altered or enhanced phenotypes and traits as shown in Tables 9 and 10.

Example 3

Identification of Altered Phenotypes in Automated Greenhouse

[0136] This example illustrates screening and identification of transgenic corn plants for altered phenotypes in an automated greenhouse (AGH). The apparatus and the methods for automated phenotypic screening of plants are disclosed, for example, in U.S. Patent Publication No. 2011/0135161, which is incorporated herein by reference in its entirety.

[0137] Corn plants were tested in three screens in the AGH under different conditions including non-stress, nitrogen deficit, and water deficit stress conditions. All screens began with non-stress conditions during days 0-5 germination phase, after which the plants were grown for 22 days under the screen-specific conditions shown in Table 2.

TABLE-US-00002 TABLE 2 Description of the three AGH screens for corn plants Screen specific Germination phase phase Screen Description (5 days) (22 days) Non-stress well watered 55% VWC 55% VWC sufficient nitrogen water 8 mM nitrogen Water deficit limited watered 55% VWC 30% VWC sufficient nitrogen water 8 mM nitrogen Nitrogen deficit well watered 55% VWC 55% VWC low nitrogen water 2 mM nitrogen

[0138] Water deficit is defined as a specific Volumetric Water Content (VWC) that is lower than the VWC of a non-stressed plant. For example, a non-stressed plant might be maintained at 55% VWC, and the VWC for a water-deficit assay might be defined around 30% VWC. Data were collected using visible light and hyperspectral imaging as well as direct measurement of pot weight and amount of water and nutrient applied to individual plants on a daily basis.

[0139] Nitrogen deficit is defined (in part) as a specific mM concentration of nitrogen that is lower than the nitrogen concentration of a non-stressed plant. For example, a non-stressed plant might be maintained at 8 mM nitrogen, while the nitrogen concentration applied in a nitrogen-deficit assay might be maintained at a concentration of 2 mM.

[0140] Up to ten parameters were measured for each screen. The visible light color imaging based measurements are: biomass, canopy area, and plant height. Biomass (Bmass) is defined as the estimated shoot fresh weight (g) of the plant obtained from images acquired from multiple angles of view. Canopy Area (Cnop) is defined as leaf area as seen in a top-down image (mm.sup.2). Plant Height (PlntH) refers to the distance from the top of the pot to the highest point of the plant derived from a side image (mm). Anthocyanin score and area, chlorophyll score and concentration, and water content score are hyperspectral imaging-based parameters. Anthocyanin Score (AntS) is an estimate of anthocyanin in the leaf canopy obtained from a top-down hyperspectral image. Anthocyanin Area (AntA) is an estimate of anthocyanin in the stem obtained from a side-view hyperspectral image. Chlorophyll Score (ClrpS) and Chlorophyll Concentration (ClrpC) are both measurements of chlorophyll in the leaf canopy obtained from a top-down hyperspectral image, where Chlorophyll Score measures in relative units, and Chlorophyll Concentration is measured in parts per million (ppm) units. Water Content Score (WtrCt) is a measurement of water in the leaf canopy obtained from a top-down hyperspectral image. Water Use Efficiency (WUE) is derived from the grams of plant biomass per liter of water added. Water Applied (WtrAp) is a direct measurement of water added to a pot (pot with no hole) during the course of an experiment.

[0141] These physiological screen runs were set up so that tested transgenic lines were compared to a control line. The collected data were analyzed against the control using % delta and certain p-value cutoff. Tables 3-5 are summaries of transgenic corn plants comprising the disclosed recombinant DNA constructs with altered phenotypes under non stress, nitrogen deficit, and water deficit conditions, respectively.

[0142] The test results are represented by three numbers: the first number before letter "p" denotes number of events with an increase in the tested parameter at p.ltoreq.0.1; the second number before letter "n" denotes number of events with a decrease in the tested parameter at p.ltoreq.0.1; the third number before letter "t" denotes total number of transgenic events tested for a given parameter in a specific screen. The increase or decrease is measured in comparison to non-transgenic control plants. A designation of "-" indicates that it has not been tested. For example, 2p1n5t indicates that 5 transgenic plant events were screened, of which 2 events showed an increase, and 1 showed a decrease of the measured parameter.

TABLE-US-00003 TABLE 3 Summary of transgenic corn plants with altered phenotypes in AGH non-stress screens GeneID AntS Bmass Cnop ClrpS PlntH WtrAp WtrCt WUE ClrpC AntA T5MON15 0p1n5t 0p2n5t 0p1n5t 0p0n5t 0p1n5t 0p1n5t 0p0n5t 0p2n5t -- -- T5MON02 0p0n5t 1p0n5t 0p0n5t 1p0n5t 1p0n5t 0p0n5t -- 1p0n5t -- -- T5MON23 1p0n5t 0p0n5t 0p0n5t 0p2n5t 0p1n5t 1p1n5t -- 0p0n5t -- -- T5MON17 0p0n5t 0p1n5t 0p4n5t 1p0n5t 0p0n5t 0p0n5t 1p1n5t 0p1n5t -- -- T5MON03 0p0n5t 0p0n5t 1p0n5t -- 0p1n5t 1p0n5t -- 0p0n5t 0p0n5t 0p0n5t T5MON05 1p1n10t 0p2n10t 0p2n10t -- 1p3n10t 0p2n10t -- 0p1n10t 2p0n10t 0p1n10t T5MON16 0p1n5t 0p1n5t 0p1n5t -- 1p0n5t 0p1n5t -- 0p2n5t 0p0n5t -- T5MON26 0p0n5t 1p2n5t 0p1n5t -- 0p1n5t 0p0n5t -- 1p1n5t 0p0n5t -- T5MON27 1p1n7t 0p0n7t 3p0n7t 0p1n7t 0p1n7t 0p1n7t 0p0n7t 0p0n7t -- -- T5MON06 0p0n8t 1p0n8t 1p0n8t 0p0n8t 0p0n8t 0p0n8t 0p0n8t 0p0n8t -- -- T5MON28 0p0n5t 0p1n5t 0p1n5t -- 0p2n5t Ip2n5t -- 0p2n5t 0p0n5t -- T5MON19 0p1n5t 0p1n5t 0p1n5t -- 0p2n5t 0p1n5t -- 0p2n5t 0p0n5t 0p1n5t T5MON29 2p1n5t 0p2n5t 0p1n5t -- 0p2n5t 0p0n5t -- 0p2n5t 0p0n5t 0p0n5t T5MON08 0p1n5t 0p1n5t 0p0n5t -- 0p1n5t 0p0n5t -- 0p1n5t 0p1n5t -- T5MON14 0p0n5t 1p0n5t 0p0n5t -- 2p0n5t 1p0n5t -- 1p0n5t 0p0n5t --

TABLE-US-00004 TABLE 4 Summary of transgenic corn plants with altered phenotypes in AGH nitrogen-deficit screens GeneID AntS Bmass Cnop ClrpS PlntH WtrAp WtrCt WUE ClrpC AntA T5MON01 -- 3p0n5t 3p0n5t -- 2p0n5t 3p0n5t -- 1p0n5t -- 1p0n5t T5MON02 0p0n5t 0p0n5t 0p0n5t 3p0n5t 1p0n5t 1p2n5t 0p0n5t 1p0n5t -- -- T5MON03 0p0n5t 0p0n5t 1p0n5t -- 0p1n5t 0p0n5t -- 0p0n5t 0p1n5t 0p0n5t T5MON04 0p0n10t 0p0n10t 0p1n10t -- 1p1n10t 0p2n10t -- 0p0n10t 0p0n10t 1p0n10t T5MON05 1p0n10t 0p0n10t 0p3n10t -- 0p3n10t 1p1n10t -- 1p1n10t Ip0n10t 0p2n10t T5MON06 0p1n8t 0p1n8t 0p1n8t 0p1n8t 0p5n8t 1p1n8t 0p1n8t 0p3n8t -- -- T5MON07 0p0n5t 0p0n5t 0p0n5t -- 0p0n5t 1p0n5t -- 0p0n5t 0p1n5t 1p0n5t T5MON08 0p2n5t 4p0n5t 1p0n5t -- 0p0n5t 3p0n5t -- 4p0n5t 2p0n5t -- T5MON14 2p0n5t 0p3n5t 0p3n5t -- 0p1n5t 0p3n5t -- 0p2n5t 0p1n5t -- T5MON15 0p1n5t 0p0n5t 0p2n5t 0p0n5t 0p4n5t 0p3n5t 0p0n5t 0p0n5t -- -- T5MON16 1p0n5t 0p1n5t 0p1n5t -- 0p2n5t 0p1n5t -- 0p1n5t 1p0n5t -- T5MON17 0p1n5t 0p3n5t 1p1n5t 2p0n5t 0p4n5t 1p1n5t 0p3n5t 0p4n5t -- -- T5MON19 0p1n5t 0p0n5t 0p2n5t -- 1p0n5t 0p5n5t -- 0p0n5t 0p0n5t 0p3n5t T5MON23 0p0n5t 4p0n5t 2p0n5t 0p1n5t 2p0n5t 4p0n5t 1p0n5t 3p0n5t -- -- T5MON25 0p1n8t 2p0n8t 0p1n8t -- 2p1n8t 0p4n8t -- 3p0n8t 3p1n8t 0p0n8t T5MON26 0p0n5t 0p2n5t 0p3n5t -- 0p2n5t 0p1n5t -- 0p2n5t 0p0n5t -- T5MON27 1p0n7t 0p1n7t 0p0n7t 0p0n7t 0p2n7t 0p0n7t 0p0n7t 0p1n7t -- -- T5MON28 0p0n5t 0p2n5t 0p0n5t -- 0p3n5t 0p1n5t -- 0p1n5t 1p0n5t -- T5MON29 0p0n5t 0p0n5t 0p2n5t -- 0p1n5t 0p2n5t -- 1p0n5t 1p0n5t 0p0n5t

TABLE-US-00005 TABLE 5 Summary of transgenic corn plants with altered phenotypes in AGH water-deficit screens GeneID AntS Bmass Cnop ClrpS PlntH WtrAp WtrCt WUE ClrpC AntA T5MON01 0p0n5t 0p2n5t 0p3n5t -- 1p1n5t 0p3n5t -- 0p0n5t 0p1n5t 2p0n5t T5MON02 0p0n5t 1p1n5t 0p1n5t 0p3n5t 0p1n5t 1p2n5t 0p1n5t 0p0n5t -- -- T5MON03 0p0n5t 0p1n5t 0p0n5t -- 0p0n5t 0p3n5t -- 2p0n5t 0p0n5t 1p0n5t T5MON04 0p1n10t 3p1n10t 4p0n10t -- 1p1n10t 1p1n10t -- 3p0n10t 3p0n10t 0p0n10t T5MON05 0p1n10t 0p0n10t 1p0n10t -- 1p1n10t 2p2n10t -- 0p0n10t 3p0n10t 0p4n10t T5MON06 0p0n8t 0p1n8t 0p0n8t 0p2n8t 0p1n8t 0p3n8t 0p2n8t 0p1n8t -- -- T5MON07 1p0n5t 0p0n5t 0p1n5t -- 0p1n5t 1p1n5t -- 0p0n5t 1p1n5t 1p0n5t T5MON08 1p0n5t 0p0n5t 0p0n5t -- 0p0n5t 0p0n5t -- 0p0n5t 0p0n5t -- T5MON14 0p0n5t 2p1n5t 3p1n5t -- 0p1n5t 0p1n5t -- 2p0n5t 0p0n5t -- T5MON15 0p3n5t 2p0n5t 3p0n5t 1p0n5t 2p0n5t 3p0n5t 0p0n5t 2p0n5t -- -- T5MON16 0p0n5t 0p1n5t 0p1n5t -- 0p0n5t 0p2n5t -- 0p1n5t 0p1n5t -- T5MON17 0p0n5t 0p0n5t 0p0n5t 1p0n5t 0p0n5t 1p0n5t 0p1n5t 0p0n5t -- -- T5MON19 1p0n5t 1p0n5t 2p0n5t -- 1p0n5t 3p0n5t -- 0p0n5t 0p2n5t 0p1n5t T5MON23 0p0n5t 4p0n5t 3p0n5t 1p0n5t 4p0n5t 5p0n5t 0p0n5t 0p0n5t -- -- T5MON25 0p1n8t 3p0n8t 1p0n8t -- 2p0n8t 0p0n8t -- 3p0n8t 2p0n8t 0p1n8t T5MON26 0p1n5t 0p2n5t 0p2n5t -- 0p1n5t 0p4n5t -- 0p0n5t 0p0n5t -- T5MON27 0p2n7t 1p0n7t 2p0n7t 0p0n7t 0p1n7t 3p0n7t 1p0n7t 0p0n7t -- -- T5MON28 1p0n5t 2p1n5t 2p0n5t -- 0p1n5t 3p0n5t -- 0p1n5t 1p0n5t -- T5MON29 2p0n5t 3p1n5t 1p1n5t -- 1p1n5t 2p1n5t -- 2p1n5t 0p0n5t 0p0n5t

Example 4

Evaluation of Transgenic Plants for Trait Characteristics

[0143] Trait assays were conducted to evaluate trait characteristics and phenotypic changes in transgenic plants as compared to non-transgenic controls. Corn and soybean plants were grown in field and greenhouse conditions. Up to 18 parameters were measured for corn in phenology, morphometrics, biomass, and yield component studies at certain plant developmental stages. For root assays, soybean plants were grown in the greenhouse in transparent nutrient medium to allow the root system to be imaged and analyzed.

[0144] Corn developmental stages are defined by the following development criteria:

[0145] Developed leaf: leaf with a visible leaf collar;

[0146] V-Stages: Number of developed leaves on a corn plant corresponds to the plant's vegetative growth stage--i.e., a V6 stage corn plant has 6 developed (fully unfolded) leaves;

[0147] R1 (Silking): Plants defined as R1 must have one or more silks extending outside the husk leaves. Determining the reproductive stage of the crop plant at R1 or later is based solely on the development of the primary ear;

[0148] R3 (Milk): Typically occurs 18-22 days after silking depending on temperature and relative maturity. Kernels are usually yellow in color and the fluid inside each kernel is milky white;

[0149] R6 (Physiological maturity): Typically occurs 55-65 days after silking (depending on temperature and relative maturity group of the germplasm being observed). Kernels have reached their maximum dry matter accumulation at this point, and kernel moisture is approximately 35%.

[0150] Soybean developmental stages are defined by criteria as following:

[0151] Fully developed trifoliate leaf node: A leaf is considered completely developed when the leaf at the node immediately above it has unrolled sufficiently so the two edges of each leaflet are no longer touching. At the terminal node on the main stem, the leaf is considered completely developed when the leaflets are flat and similar in appearance to older leaves on the plant;

[0152] VC: Cotyledons and Unifoliolates are fully expanded;

[0153] R1: Beginning of flowering--i.e., one open flower at any node on the main stem.

[0154] Table 6 describes the trait assays. TraitRefID is the reference ID of each trait assay. Trait Name is the descriptive name of the assay. Trait Description describes what the assay measures, and how the measurement is conducted. Direction For Positive Call indicates whether an increase or decrease in the measurement quantity corresponds to a "positive" call in the assay results.

TABLE-US-00006 TABLE 6 Description of Trait Assays. Direction For Positive TraitRefID Trait Assay Name Description Call DOV12 Days from Planting to number of days from the date of planting to the decrease V12 date when 50% of the plants in a plot reaches V12 stage. P50DR1 Days to 50% Pollen number of days from the date of planting to the decrease Shedding date when 50% of the plants in a plot reaches Pollen Shed stage. S50DR1 Days to 50% Visible Silk number of days from the date of planting to the decrease date when 50% of the plants in a plot reaches visible Silking (R1) stage. STDIR3 Stalk Diameter at R3 plot average of the stalk diameter of a plant. It increase measures maximal "long" axis in the middle of the internode above first visible node. Measurement is taken at R3 stage. KRNR6 Kernel Row Number at plot average of the number of rows of kernels on increase R6 an ear, by counting around the circumference of the ear. Measurement is taken at R6 stage. KRLR6 Kernels per Row (also known as rank number) the plot average of increase Longitudinally at R6 the number of kernels per row longitudinally. It is calculated as the ratio of (total kernel count per ear)/(kernel row number). Measurement is taken at R6 stage. LFTNR3 Leaf Tip Number at R3 plot average of the number of leaves per plant, by increase counting the number of leaf tips. Measurement is taken at R3 stage. PLTHGR Plant Height Growth plot average of growth rate of a plant from V6 to increase Rate from V6 to V12 V12 stage. It is calculated as (Plant Height measured at V12-Plant Height measured at V6)/ Days between measurements. PHTR3 Plant Height at R3 plot average of plant height. It measures from soil decrease line to base of highest collared leaf. Measurement is taken at R3 stage. EAR6 Ear Area at R6 plot average of size of area of a ear from a 2- increase dimentional view. The measurement is done through imaging of ear, including kernels and void. Typically 10 representative ears are measured per plot. Measurement is taken at R6 stage. EDR6 Ear Diameter at R6 plot average of the ear diameter. It measures increase maximal "wide" axis over the ear on the largest section of the ear. Measurement is taken at R6 stage. EDWR1 Ear Dry Weight at R6 plot average of the ear dry weight of a plant. increase Measurement is taken at R6 stage. ELR6 Ear Length at R6 plot average of the length of ear. It measures from increase tip of ear in a straight line to the base at the ear node. Measurement is taken at R6 stage. ETVR6 Ear Tip Void Percentage plot average of area percentage of void at the top decrease at R6 30% area of a ear, from a 2-dimentional view. The measurement is done through imaging of ear, including kernels and void. Typically 10 representative ears are measured per plot. Measurement is taken at R6 stage. EVR6 Ear Void Percentage at plot average of area percentage of void on a ear, decrease R6 from a 2-dimentional view. The measurement is done through imaging of ear, including kernels and void. Typically 10 representative ears are measured per plot. Measurement is taken at R6 stage. KPER6 Kernels per Ear at R6 plot average of the number of kernels per ear. It is increase calculated as (total kernel weight/(Single Kernel Weight * total ear count), where total kernel weight and total ear count are measured from ear samples from an area between 0.19 to 10 square meters, and Single Kernel Weight (SKWTR6) is described below. Measurement is taken at R6 stage. SKWTR6 Single Kernel Weight at plot average of weight per kernel. It is calculated increase R6 as the ratio of (sample kernel weight adjusted to 15.5% moisture)/(sample kernel number). The sample kernel number ranges from 350 to 850. Measurement is taken at R6 stage. RBPN Root Branch Point number of root branch tip points of a plant. The increase Number at VC or V2 measurement is done through imaging of the root system of a plant grown in a transparent Gelzan .TM. gum gel nutrient medium to VC stage for soybean, or to V2 stage for corn. The root system image is skeletonized for the root length measurement. Up to 40 images are taken at various angles around the root vertical axis and measurement is averaged over the images. Gelzan is a trademark of CP Kelco U.S., Inc. RTL Root Total Length at VC cumulative length of roots of a plant, as if the roots increase or V2 were all lined up in a row. The measurement is done through imaging of the root system of a plant grown in a transparent Gelzan .TM. gum gel nutrient medium to VC stage for soybean, or to V2 stage for corn. The root system image is skeletonized for the root length measurement. Up to 40 images are taken at various angles around the root vertical axis and measurement is averaged over the images. Gelzan is a trademark of CP Kelco U.S., Inc.

[0155] These trait assays were set up so that the tested transgenic lines were compared to a control line. The collected data were analyzed against the control, and positives were assigned if there was a p-value of 0.2 or less. Tables 7, 8 and 9 are summaries of transgenic plants comprising the disclosed recombinant DNA constructs for corn phenology and morphometrics assays, corn yield/trait component assays, and soybean root assays, respectively.

[0156] The test results are represented by three numbers: the first number before letter "p" denotes number of tests of events with a "positive" change as defined in Table 6; the second number before letter "n" denotes number of tests of events with a "negative" change which is in the opposite direction of "positive" as defined in Table 6; the third number before letter "t" denotes total number of tests of transgenic events for a specific assay for a given gene. The "positive" or "negative" change is measured in comparison to non-transgenic control plants. A designation "-" indicates that it has not been tested. For example, 2p1n5t indicates that 5 transgenic plant events were tested, of which 2 events showed a "positive" change and 1 showed a "negative" change of the measured parameter. The assay is indicated with its TraitRefiD as in Table 6. Note that two constructs of gene T5MON21 were tested in some assays, and the results are listed as T5MON21 and T5MON21x.

TABLE-US-00007 TABLE 7 Summary of assay results for corn phenology and morphometrics assays Gene_ID P50DR1 S50DR1 KRNR6 KRLR6 LFTNR3 DOV12 PHTR3 STDIR3 PLTHGR T5MON01 2p0n4t 0p0n4t -- -- -- 2p0n4t -- -- 5p0n12t T5MON02 6p0n11t 2p0n11t 0p1n6t 3p0n6t 0p0n3t -- -- -- -- T5MON03 -- -- 1p0n4t 1p0n4t -- -- -- -- -- T5MON06 1p0n6t 0p0n5t -- -- -- -- 2p0n5t 1p0n5t -- T5MON08 1p0n4t 0p0n4t 2p0n3t 1p1n3t -- -- -- -- -- T5MON16 0p0n8t 4p2n8t 0p0n4t 0p1n4t 0p0n4t -- -- -- -- T5MON17 -- -- 0p3n4t 0p1n4t -- -- -- -- -- T5MON19 2p1n4t 3p0n4t -- -- 4p0n4t -- -- -- -- T5MON20 2p0n4t 0p0n4t 0p1n2t 0p0n2t 0p1n2t -- -- -- -- T5MON21 0p0n4t 0p0n4t 0p1n3t 1p0n3t -- -- -- -- -- T5MON21x 0p3n5t 0p4n5t 0p2n3t 0p0n3t -- -- -- -- -- T5MON23 2p1n7t 1p0n6t -- -- 0p0n1t -- -- -- -- T5MON26 2p0n11t 2p0n11t 2p0n6t 0p0n6t 0p0n3t -- -- -- -- T5MON27 0p0n4t 1p0n5t 0p0n2t 0p0n2t 0p0n2t -- -- -- -- T5MON28 2p0n11t 2p3n11t 0p3n7t 0p2n4t 0p2n4t 0p2n3t -- -- -- T5MON29 -- -- 0p0n4t 0p0n4t -- -- -- -- --

TABLE-US-00008 TABLE 8 Summary of assay results for corn trait component assays GeneID EAR6 EDR6 ELR6 ETVR6 EVR6 KPER6 SKWTR6 EDWR1 T5MON02 1p0n3t 0p0n3t 2p0n3t 1p0n3t 1p0n3t 2p0n6t 0p0n6t 1p0n3t T5MON03 -- -- -- -- -- 1p0n4t 0p1n4t -- T5MON08 1p0n6t 1p0n6t 1p0n6t 0p1n6t 0p1n6t 1p1n6t 1p2n6t -- T5MON16 2p0n3t 0p0n3t 2p0n3t 0p1n3t 0p1n3t 0p0n7t 2p0n7t 4p0n7t T5MON17 -- -- -- -- -- 0p1n4t 0p0n4t -- T5MON19 -- -- -- -- -- 0p0n4t 0p0n4t -- T5MON20 0p1n2t 0p2n2t 0p1n2t 1p0n2t 1p0n2t 0p0n4t 0p1n4t 0p0n2t T5MON21 1p0n3t 1p0n3t 1p0n3t 0p1n3t 0p0n3t 1p1n3t 1p0n3t -- T5MON21x 0p0n3t 1p1n3t 0p0n3t 0p3n3t 0p3n3t 0p0n3t 0p0n3t -- T5MON26 2p0n3t 2p0n3t 2p0n3t 0p0n3t 0p0n3t 1p0n6t 0p1n6t 0p0n3t T5MON27 0p0n2t 0p0n2t 1p0n2t 0p0n2t 0p0n2t 0p1n2t 0p0n2t -- T5MON28 0p2n3t 0p1n3t 0p2n3t 0p0n3t 0p0n3t 0p5n4t 5p0n7t 0p1n7t T5MON29 -- -- -- -- -- 0p0n4t 0p2n4t --

TABLE-US-00009 TABLE 9 Summary of assay results for soybean root assays Gene_ID RBPN RTL T5MON12 0p0n4t 1p0n4t T5MON13 3p0n4t 3p0n4t

Example 5

Phenotypic Evaluation of Transgenic Plants in Field Trials for Increased Nitrogen Use Efficiency, Increased Water Use Efficiency, and Increased Yield

[0157] Corn field trials were conducted to identify genes that can improve nitrogen use efficiency (NUE) under nitrogen limiting conditions leading to increased yield performance as compared to non transgenic controls. For the Nitrogen field trial results shown in Table 10, each field was planted under nitrogen limiting condition (60 lbs/acre), and corn ear weight or yield was compared to non-transgenic control plants.

[0158] Corn field trials were conducted to identify genes that can improve water use efficiency (WUE) under water limiting conditions leading to increased yield performance as compared to non transgenic controls. Results of the water use efficiency trials conducted under managed water limiting conditions are shown in Table 10, and the corn ear weight or yield was compared to non-transgenic control plants.

[0159] Corn and soybean field trials were conducted to identify genes that can improve broad-acre yield (BAY) under standard agronomic practice. Results of the broad-acre yield trials conducted under standard agronomic practice are shown in Table 10, and the corn or soybean yield was compared to non-transgenic control plants.

[0160] Table 10 provides a list of genes that produce transgenic plants having increased nitrogen use efficiency (NUE), increased water use efficiency (WUE), and/or increased broad-acre yield (BAY) as compared to a control plant. Polynucleotide sequences in constructs with at least one event showing significant yield or ear weight increase across multiple locations at p.ltoreq.0.2 are included. The genes were expressed with constitutive promoters unless noted otherwise under the "Specific Expression Pattern" column. A promoter of a specific expression pattern was chosen over a constitutive promoter, based on the understanding of the gene function, or based on the observed lack of significant yield increase when the gene was expressed with constitutive promoter. The elements of Table 10 are described as follows: "Crop" refers to the crop in trial, which is either corn or soybean; "Condition" refers to the type of field trial, which is BAY for broad acre yield trial under standard agronomic practice (SAP), WUE for water use efficiency trial, and NUE for nitrogen use efficiency trial; "Specific Expression Pattern" refers to the expected expression pattern or promoter type, instead of constitutive; "Gene ID" refers to the gene identifier as defined in Table 1; "Yield results" refers to the recombinant DNA in a construct with at least one event showing significant yield increase at p.ltoreq.0.2 across locations. The first number refers to the number of tests of events with significant yield or ear weight increase, whereas the second number refers to the total number of tests of events for each recombinant DNA in the construct. Typically 4 to 8 distinct events per construct are tested.

TABLE-US-00010 TABLE 10 Recombinant DNA with protein-coding genes for increased nitrogen use efficiency, increased water use efficiency and increased yield Crop Condition Specific Expression Pattern Gene ID Yield results corn BAY T5MON01 1/7 corn BAY T5MON04 3/8 corn BAY root specific T5MON05 4/28 corn BAY root specific T5MON06 7/44 corn WUE root specific T5MON06 1/6 corn BAY seed specific T5MON08 1/18 corn WUE seed specific T5MON08 3/5 corn BAY T5MON14 1/15 corn WUE T5MON14 2/6 corn BAY root specific T5MON15 2/8 corn BAY T5MON16 2/20 corn NUE T5MON16 5/11 corn BAY T5MON17 4/16 corn BAY T5MON19 8/19 corn WUE meristem & cob enhanced T5MON21 1/7 corn BAY seed preferred T5MON23 3/27 corn NUE seed preferred T5MON23 1/14 corn BAY meristem & endosperm T5MON27 2/24 preferred corn BAY T5MON28 1/14 soybean BAY T5MON09 3/12 soybean BAY T5MON10 3/13 soybean BAY T5MON11 6/23 soybean BAY T5MON18 2/28 soybean BAY seed preferred T5MON24 3/14

[0161] Table 11 provides a list of polynucleotide sequences of promoters with specific expression patterns. To convey the specific expression patterns, choices of promoters are not limited to those listed in Table 11.

TABLE-US-00011 TABLE 11 Promoter sequences and expression patterns Nucleotide SEQ ID NO. Promoter Expression Pattern 93 seed preferred 94 root preferred 95 meristem & endosperm preferred 96 root specific 97 endosperm specific 98 endosperm specific 99 seed specific 100 seed preferred 101 meristem & cob enhanced

Example 6

Homolog Identification

[0162] This example illustrates the identification of homologs of proteins encoded by the DNA sequences identified in Table 1, which were used to provide transgenic seed and plants having enhanced agronomic traits. From the sequences of the homolog proteins, corresponding homologous DNA sequences can be identified for preparing additional transgenic seeds and plants with enhanced agronomic traits.

[0163] An "All Protein Database" was constructed of known protein sequences using a proprietary sequence database and the National Center for Biotechnology Information (NCBI) non-redundant amino acid database (nr.aa). For each organism from which a polynucleotide sequence provided herein was obtained, an "Organism Protein Database" was constructed of known protein sequences of the organism; it is a subset of the All Protein Database based on the NCBI taxonomy ID for the organism.

[0164] The All Protein Database was queried using amino acid sequences provided in Table 1 using NCBI "blastp" program with E-value cutoff of 1e-8. Up to 1000 top hits were kept, and separated by organism names. For each organism other than that of the query sequence, a list was kept for hits from the query organism itself with a more significant E-value than the best hit of the organism. The list contains likely duplicated genes of the polynucleotides provided herein, and is referred to as the Core List. Another list was kept for all the hits from each organism, sorted by E-value, and referred to as the Hit List.

[0165] The Organism Protein Database was queried using polypeptide sequences provided in Table 1 using NCBI "blastp" program with E-value cutoff of 1e-4. Up to 1000 top hits were kept. A BLAST searchable database was constructed based on these hits, and is referred to as "SubDB". SubDB is queried with each sequence in the Hit List using NCBI "blastp" program with E-value cutoff of 1e-8. The hit with the best E-value was compared with the Core List from the corresponding organism. The hit is deemed a likely ortholog if it belongs to the Core List, otherwise it is deemed not a likely ortholog and there is no further search of sequences in the Hit List for the same organism. Homologs with at least 95% identity over 95% of the length of the polypeptide sequences provided in Table 1 are reported below in Tables 12 and 13.

[0166] Table 12 provides a list of homolog genes, the elements of which are described as follows: "PEP SEQ ID NO." identifies an amino acid sequence. "Homolog ID" refers to an alphanumeric identifier, the numeric part of which is the NCBI Genbank GI number; and "Gene Name and Description" is a common name and functional description of the gene. Table 13 describes the correspondence between the protein-coding genes in Table 1 and their homologs, and the level of protein sequence alignment between the gene and its homolog.

TABLE-US-00012 TABLE 12 Homologous gene information PEP SEQ ID NO. Homolog ID Gene Name and Description 59 gi_194698766 gi|194698766|gb|ACF83467.1| unknown [Zea mays] 60 gi_285013667 gi|285013667|gb|ADC32810.1| pyruvate orthophosphate dikinase [Zea mays] 61 gi_242063426 gi|242063426|ref|XP_002453002.1| hypothetical protein SORBIDRAFT_04g036440 [Sorghum bicolor] 62 gi_26248016 gi|26248016|ref|NP_754056.1| glutamate dehydrogenase [Escherichia coli CFT073] 63 gi_223975805 gi|223975805|gb|ACN32090.1| unknown [Zea mays] 64 gi_162457981 gi|162457981|ref|NP_001105435.1| nuclear transcription factor Y subunit B [Zea mays] 65 gi_15802172 gi|15802172|ref|NP_288194.1| glutamate dehydrogenase [Escherichia coli O157:H7 EDL933] 66 gi_146126 gi|146126|gb|AAA23868.1| glutamate dehydrogenase [Escherichia coli] 67 gi_193806357 gi|193806357|sp|P11155|Pyruvate, phosphate dikinase 1 [Zea mays] 68 gi_115840 gi|115840|sp|P25209| Nuclear transcription factor Y subunit B [Zea mays] 69 gi_284921680 gi|284921680|emb|CBG34752.1| NADP-specific glutamate dehydrogenase [Escherichia coli 042] 70 gi_188039906 gi|188039906|gb|ACD47129.1| EREBP/AP2 transcription factor [Glycine max] 71 gi_162462936 gi|162462936|ref|NP_001106052.1| transcription factor subunit NF-YB2 [Zea mays] 72 gi_19423910 gi|19423910|gb|AAL87324.1| unknown protein [Arabidopsis thaliana] 73 gi_165874954 gi|165874954|gb|ABY68378.1| At1g04370 [Arabidopsis thaliana] 74 gi_22093879 gi|22093879|dbj|BAC07164.1|hypothetical protein [Oryza sativa Japonica Group] 75 gi_226508884 gi|226508884|ref|NP_001150756.1| homeobox-leucine zipper protein ATHB-4 [Zea mays] 76 gi_226492268 gi|226492268|ref|NP_001142481.1| hypothetical protein LOC100274701 [Zea mays] 77 gi_15219717 gi|15219717|ref|NP_171932.1| ATERF14 (Ethylene- responsive element binding factor 14); DNA binding/ transcription factor [Arabidopsis thaliana] 78 gi_4966365 gi|4966365|gb|AAD34696.1|Eukaryotic protein kinase domain [Arabidopsis thaliana] 79 gi_65874952 gi|165874952|gb|ABY68377.1| At1g04370 [Arabidopsis thaliana] 80 gi_21592545 gi|21592545|gb|AAM64494.1| glycyl tRNA synthetase, putative [Arabidopsis thaliana] 81 gi_222424295 gi|222424295|dbj|BAH20104.1| AT3G30390 [Arabidopsis thaliana] 82 gi_226503589 gi|226503589|ref|NP_001141333.1| CAAT box binding protein1 [Zea mays] 83 gi_219885341 gi|219885341|gb|ACL53045.1| unknown [Zea mays] 84 gi_3980050 gi|3980050|emb|CAA41635.1| glutamate dehydrogenase (NADP+) [Chlorella sorokiniana] 85 gi_168586 gi|168586|gb|AAA33498.1| pyruvate, orthophosphate dikinase [Zea mays] 86 gi_162460730 gi|162460730|ref|NP_001105738.1| pyruvate, phosphate dikinase 1, chloroplastic precursor [Zea mays] 87 gi_21553511 gi|21553511|gb|AAM62604.1| growth factor like protein [Arabidopsis thaliana] 88 gi_2208903 gi|2208903|dbj|BAA20519.1| ascorbate oxidase [Arabidopsis thaliana] 89 gi_118546 gi|118546|sp|P28998|NADP-specific glutamate dehydrogenase [Chlorella sorokiniana] 90 gi_165874950 gi|165874950|gb|ABY68376.1| At1g04370 [Arabidopsis thaliana] 91 gi_162459403 gi|162459403|ref|NP_001105725.1| glutamine synthetase, chloroplastic precursor [Zea mays] 92 gi_162463830 gi|162463830|ref|NP_001105461.1| proliferating cell nuclear antigen [Zea mays]

TABLE-US-00013 TABLE 13 Correspondence of Genes and Homologs Percent Percent Gene Homolog Percent Gene ID Homolog ID Coverage Coverage Identity T5MON04 gi_21592545 95 100 99 T5MON05 gi_2208903 96 100 99 T5MON07 gi_21553511 100 100 99 T5MON08 gi_222424295 100 100 99 T5MON09 gi_15219717 100 100 99 T5MON09 gi_165874950 98 100 99 T5MON09 gi_165874952 98 100 98 T5MON09 gi_165874954 98 100 98 T5MON10 gi_19423910 99 100 100 T5MON13 gi_4966365 98 99 99 T5MON15 gi_118546 100 86 99 T5MON15 gi_3980050 100 86 99 T5MON16 gi_146126 100 100 99 T5MON16 gi_26248016 100 100 99 T5MON16 gi_15802172 100 100 99 T5MON16 gi_284921680 100 100 99 T5MON18 gi_188039906 100 100 99 T5MON19 gi_22093879 100 90 98 T5MON20 gi_219885341 100 100 99 T5MON20 gi_285013667 100 100 99 T5MON20 gi_168586 98 100 99 T5MON20 gi_193806357 98 100 99 T5MON20 gi_162460730 98 100 97 T5MON21 gi_162463830 100 100 98 T5MON21 gi_242063426 100 100 98 15M0N22 gi_162463830 100 100 98 15M0N22 gi_242063426 100 100 98 15M0N27 gi_162457981 100 100 99 15M0N27 gi_115840 100 99 99 15M0N27 gi_226503589 100 100 96 15M0N27 gi_162462936 100 100 96 15M0N28 gi_226508884 96 75 100 15M0N29 gi_194698766 100 100 99 15M0N29 gi_162459403 100 100 99 T5MON30 gi_223975805 100 100 99 T5MON30 gi_226492268 100 100 98

Example 7

Use Of Site-Directed Integration to Introduce Transgenes or Modulate Expression of Endogenous Genes in Plants

[0167] As introduced above, a DNA sequence comprising a transgene(s), expression cassette(s), etc., such as one or more coding sequences of genes identified in Tables 1 and 12, or homologs thereof, may be inserted or integrated into a specific site or locus within the genome of a plant or plant cell via site-directed integration. A DNA sequence comprising one or more coding sequences of genes identified in Tables 1 and 12, or homologs thereof, may be operably linked to a promoter and/or other regulatory elements, such as a 5' and/or 3' untranslated region (UTR), enhancer, intron, and/or terminator region, which may each be native, non-native, synthetic and/or heterologous to the coding sequence or plant host cell. Recombinant DNA constructs and molecules of this disclosure may thus include a donor template having an insertion sequence comprising at least one transgene, expression cassette, or other DNA sequence for insertion into the genome of the plant or plant cell. Such donor template for site-directed integration may further include one or two homology arms flanking the insertion sequence to promote insertion of the insertion sequence at the desired site or locus. Any site or locus within the genome of a plant may be chosen for site-directed integration of the insertion sequence.

[0168] Several methods for site-directed integration are known in the art involving different proteins (or complexes of proteins and/or guide RNA) that cut the genomic DNA to produce a double strand break (DSB) or nick at a desired genomic site or locus. Examples of site-specific nucleases that may be used include zinc-finger nucleases, engineered or native meganucleases, TALE-endonucleases, and RNA-guided endonucleases (e.g., Cas9 or Cpf1). For methods using RNA-guided site-specific nucleases (e.g., Cas9 or Cpf1), the recombinant DNA construct(s) will also comprise a sequence encoding one or more guide RNAs to direct the nuclease to the desired site within the plant genome. The recombinant DNA molecules or constructs of this disclosure may further comprise an expression cassette(s) encoding a site-specific nuclease, a guide RNA, and/or any associated protein(s) to carry out the desired site-directed integration event.

[0169] The endogenous genomic loci of a plant or plant cell corresponding to the genes identified in Tables 1 and 12, or a homolog thereof, may be selected for site-specific insertion of a recombinant DNA molecule or sequence capable of modulating expression of the corresponding endogenous genes. As described above, the recombinant DNA molecule or sequence serves as a donor template for integration of an insertion sequence into the plant genome. The donor template may also have one or two homology arms flanking the insertion sequence to promote the targeted insertion event. Although a transgene, expression cassette, or other DNA sequence may be inserted into a desired locus or site of the plant genome via site-directed integration, a donor template may instead be used to replace, insert, or modify a 5' untranslated region (UTR), promoter, enhancer, intron, 3' UTR and/or terminator region of an endogenous gene, or any portion thereof, to modulate the expression level of the endogenous gene. Another method for modifying expression of an endogenous gene is by genome editing of an endogenous gene locus. For example, a targeted genome editing event may be made to disrupt or abolish a regulatory binding site for a transcriptional repressor of an endogenous gene to increase or modify expression of the endogenous gene.

[0170] For genome editing or site-specific integration of an insertion sequence of a donor template, a double-strand break (DSB) or nick is made in the selected genomic locus. The DSB or nick may be made with a site-specific nuclease, for example a zinc-finger nuclease, an engineered or native meganuclease, a TALE-endonuclease, or an RNA-guided endonuclease (for example Cas9 or Cpf1). In the presence of a donor template, the DSB or nick may be repaired by homologous recombination between the homology arms of the donor template and the plant genome, resulting in site-directed integration of the insertion sequence to make the targeted genomic modification or insertion at the site of the DSB or nick. For genes shown herein to cause or produce a desired phenotype or trait in a plant, an expression construct or transgene comprising the coding sequence of the gene may be inserted at a desired or selected site within the genome of the plant via site-directed integration as discussed above. Alternatively, the sequence of a corresponding endogenous gene may be modified via site-directed integration to augment or alter the expression level of the endogenous gene, such as by adding a promoter or intron sequence, or by modifying or replacing a 5' UTR sequence, promoter, enhancer, intron, 3' UTR sequence, and/or terminator region, or any portion thereof, of the endogenous gene.

[0171] Following transformation of a plant cell with the recombinant molecule(s) or construct(s), the resulting events are screened for site-directed insertion of the donor template insertion sequence or genome modification. Plants containing these confirmed events or modifications may then be tested for modulation of an endogenous gene, expression of an integrated transgene, and/or modification of yield traits or other phenotypes.

Sequence CWU 1

1

1011918DNAAgrobacterium tumefaciens 1atggcttcac ctaaacattt tctcgacctg tcggccgttg gcccgcagga cctgcggaca 60attcttgacg atgcgcgcgc gcgcaaggtc gccacgaagg cgggaacggc ggaaaagccg 120ctggccggca agatgctggc catgatcttc gaaaagccgt ccacccgcac ccgcgtttcc 180ttcgatgtgg gcatgcgcca gctcggcggc gagacccttt tcctgtcggg cactgaaatg 240cagctcggtc gtgcggaaac gatcggcgat accgccaagg tgctgtcgcg ttatgtggat 300gccatcatga tccgtaccac ggatcattcg cgcctgctcg agcttgccga acacgcgacg 360gtgcctgtta tcaacggcct gaccgatgat acccatccct gccagatcat ggccgacatc 420atgacgttcg aggaacatcg tggtccggtc aaaggcaaga ccattgcctg gacgggcgac 480ggcaacaacg tgctgcactc tttcgtcgag ggctccgcac gtttcggtta ccgcatgacg 540atggcggtgc cgatgggttc cgaaccgcat gacaagttca tgaactgggc ccgcaacaat 600ggcggcgaga tcgcgctgta tcatgatgcg gacaaggcgg tcgcgggtgc ggattgtgtc 660gtcaccgata cctgggtgtc catgaaccag gagcacaagg cgcgcggcca caacatcttc 720cagccctatc aggtcaatga ggccctgatg gccaaggcgc agaaggacgc gctgttcatg 780cactgcctgc cggcgcatcg cggtgaagaa gtgacggacg ccgtgatcga cggcccgcaa 840tcggtggtct tcgatgaggc ggagaaccgt ctccacgcgc agaaatccgt catcgcatgg 900tgcatgggcg tgatctga 9182645DNAArabidopsis thaliana 2atggtgaggg gcaaaactca gatgaagaga atagagaatg caacaagcag acaagtgact 60ttctccaaaa gaaggaatgg tttgttgaag aaagcctttg agctctcagt gctttgtgat 120gctgaagttt ctcttatcat cttctctcct aaaggcaaac tttatgaatt cgccagctcc 180aatatgcaag ataccataga tcgttatctg aggcatacta aggatcgagt cagcaccaaa 240ccggtttctg aagaaaatat gcagcatttg aaatatgaag cagcaaacat gatgaagaaa 300attgaacaac tcgaagcttc taaacgtaaa ctcttgggag aaggcatagg aacatgctca 360atcgaggagc tgcaacagat tgagcaacag cttgagaaaa gtgtcaaatg tattcgagca 420agaaagactc aagtgtttaa ggaacaaatt gagcagctca agcaaaagga gaaagctcta 480gctgcagaaa acgagaagct ctctgaaaag tggggatctc atgaaagcga agtttggtca 540aataagaatc aagaaagtac tggaagaggt gatgaagaga gtagcccaag ttctgaagta 600gagacgcaat tgttcattgg gttaccttgt tcttcaagaa agtag 64533021DNAArabidopsis thaliana 3atgggcgaac aacaaatctc agttacagtg cctcgtgata gaactgatga acaggcaatc 60ttggctttga agaaaggtgc acagcttttg aaatgtcgaa gaagaggaaa cccaaagttt 120tgtcctttta aactctcaat ggatgagaag tacttgattt ggtactcagg agaagaggag 180agacaattga gattgagttc tgttataacg attgttcgcg gacaaatcac accaaacttt 240cagaaacaag cacaatctga tcgaaaggaa cagtcttttt cactcattta tgcaaatgga 300gaacacactc ttgatctcat atgtaaggat aaagcacaag cagactcatg gtttaaaggt 360cttagagctg tgataacaaa gcatcataac attagaaact ctgtgaatca tcggagtagt 420aggggagctc aaagttgcat caatagtcct gcgggtttta tgcgaaggaa acagaatctt 480ggacttcttg aagagactcc tgacgtcact cagatacgta gcttatgtgg aagtccatcg 540acgttacttg aagagcggtg tttgtcgaac gggttgtcat gttcttcaga tagttttgct 600gaatctgatg ccttgggtcc agtttcttct tactatgaaa cagattatga tttcaggaac 660tcggattgtg atcgtagtac aggttcagaa ctttgcaggt tttcatcaca aagatttgct 720gcttctcctc ctctttctat cataacacag ccggttacaa gatccaatgt ccttaaggac 780attatgatat ggggagctat aacgggtctt atcgatggat cgaagaatca gaacgatgcg 840ttgtctccta agcttttgga atctgctacg atgtttgatg tacagagtat atctctaggt 900gcaaaacatg ctgcgttggt tacaagacaa ggtgaagtgt tttgttgggg gaatggaaat 960agtgggaagc ttggacttaa agttaacatc gatatcgacc atccgaaacg cgttgagtct 1020cttgaggatg ttgcagttcg atcagtggct tgtagtgatc atcaaacgtg tgctgttacg 1080gaatctggtg agctgtattt atggggaatt gatggtggaa ccattgaaca gtcaggaagc 1140cagttcttga ctcggaagat atccgatgtt cttggcggat ctttgactgt tttaagtgtt 1200gcttgtggcg cgtggcacac ggcaattgtg actagttctg gtcagctttt cacttatggt 1260agtggaactt ttggagttct tggacatggg agtcttgaga gtgttacaaa gccgaaagaa 1320gttgagtctt tgagacgcat gaaggtcata tctgtctctt gtggaccgtg gcacacggct 1380gctatagtgg aaaccgcgaa cgaccggaaa ttctacaacg ccaagagttg tggaaagctg 1440tttacttggg gagatggcga taaaggacgg ttaggacatg ctgatagcaa gagaaagctt 1500gtgccaactt gtgttactga actcattgat catgatttca taaaagtctc ttgtggatgg 1560actttaactg ttgctttaag catttcagga acggtttata caatgggaag ctcgatccac 1620ggacagttag gatgtccgcg ggcaaaggat aagtcggtaa atgttgtttt aggcaatcta 1680acacgacaat ttgtcaagga tattgcttca ggttcacatc atgtagctgt tctaacttca 1740tttggaaatg tttatacttg gggaaaaggc atgaatggac aacttggttt aggtgacgtt 1800agagatagaa actctcctgt tcttgttgag cctttaggtg ataggttggt agaaagtata 1860gcttgcgggt tgaatttgac ggctgcgatc tgtttacaca aagagatctc tttgaatgat 1920caaaccgctt gtagttcttg caaatcagct tttggattca caaggagaaa gcataactgt 1980tacaactgtg gtctattgtt ttgcaatgca tgtagtagca agaaagcggt aaatgcttcg 2040ttagcaccga ataaaagcaa gctttctcgg gtttgcgatt cttgctttga tcatctatgg 2100agtataacag agttttcaag aaatgttaag atggataatc acactccgag gatgcagatg 2160gttacacgaa gagtatctga ggatttgaca gaaaaacaat cagagaatga gatgcagaat 2220cttccacaag ctaatcgatc ttcggatgga cagcctcgat ggggacaagt ctctggtcct 2280tctctgtttc gtttcgataa gatttcgaca agttcttctt tgaatctttc agtctcagca 2340aggcgaacat cttcaacgaa gatttcaacc tcgtctgagt cgaataagat cttaaccgaa 2400gaaatcgaaa ggctaaaagc cgtgataaaa aatctccaga ggcagtgtga acttggcaat 2460gagaagatgg aagaatgtca acaagagctc gacaagacat gggaagttgc taaagaagaa 2520gcagagaaat cgaaagctgc taaagagata ataaaagcct tggcatcgaa gctccaagcg 2580aataaagaga aaccgagtaa tcctctgaag accggcattg catgcaatcc ttctcaagtc 2640tcaccaatct ttgatgactc aatgtccatt ccatacctca caccaatcac cacagctcgt 2700tcacaacacg aaaccaaaca acatgtagag aaatgtgtca cgaaatcctc gaaccgtgac 2760agcaacatta agctattagt agatgcatcg ccagccatca caagaacagg gtatctccaa 2820aacgaaacac aagactcgtc agcagaacaa gttgagcaat acgagcccgg tgtatatata 2880actttcacgg ccttgccttg cggtcagaag acgcttaaac gcgtacggtt tagtcggaag 2940aggttttcag agaaggaagc acaaagatgg tgggaagaga agcaggtttt ggtctataac 3000aagtatgatg ctgaaatata g 302142190DNAArabidopsis thaliana 4atgcgcatct tctctacatt cgtctttcat cgcagacaac aaatcttcaa tcttcgtcaa 60ttccaaacca ccacaatcct tcgcaaccca atctccatcg ctccgatcca gattccgatg 120gacgccaccg agcagtctct ccgtcaatct ctttccgaga aatcctcatc cgtcgaagct 180cagggtaacg ccgtccgagc tctcaaggct tcccgtgcag ctaaaccaga aatcgatgct 240gccattgagc aactcaacaa attgaagctc gagaaatcta ccgtcgagaa ggagcttcag 300tctattatca gtagctccgg taatggttcg cttaaccgtg aggcttttcg aaaagctgtt 360gtcaatactc tcgagcggcg tttgttctat atcccttcgt ttaagatcta cagtggcgtc 420gctggacttt ttgattatgg tcctcctggc tgtgctatta aatccaatgt tctcagcttt 480tggcgtcaac atttcattct tgaggagaac atgctagaag ttgattgtcc atgtgtgaca 540ccagaggttg ttctcaaggc atctgggcat gtagataagt tcactgatct tatggttaag 600gatgagaaaa ctggaacatg ttaccgtgct gaccacttgc tcaaggatta ttgtaccgag 660aagctggaga aagatcttac catctctgct gagaaagctg ctgaattgaa ggatgttctt 720gctgttatgg aagatttttc tcctgaacag ctaggtgcca agatcaggga gtatgggatc 780actgctccag acacgaagaa tccactctct gatccttacc cgtttaattt gatgtttcaa 840acatccattg gcccatctgg tttgattcct ggttacatgc gtcctgaaac tgctcaaggt 900atttttgtca actttaagga cttgtactat tacaatggga agaaacttcc ttttgccgcg 960gctcaaattg gtcaagcctt tagaaatgag atatctcctc gacaagggct tcttagagtt 1020cgtgaattca cgctggcaga gattgagcac ttcgttgatc ctgagaataa gtcacatcct 1080aagttctctg acgtagcaaa attggaattc cttatgttcc caagggaaga acaaatgtct 1140ggccaatctg ccaaaaaact ttgccttggt gaagctgttg ctaaaggtac tgtgaacaat 1200gaaactctag gatacttcat tgggagagtg tatcttttcc ttactcgtct tggcattgac 1260aaggaacggc tgcgcttccg tcagcatcta gcaaatgaaa tggcccacta tgcagcagat 1320tgttgggatg ctgaaattga aagttcatat gggtggattg aatgtgttgg gattgcagat 1380aggtctgctt acgacttacg tgctcactct gacaaaagtg gtactcctct tgtggctgaa 1440gagaaatttg cagaacctaa agaagtagag aaacttgtga taactcctgt gaagaaagaa 1500ctgggtcttg cattcaaggg aaatcaaaag aatgtggttg aatctttgga ggcgatgaat 1560gaggaagaag ctatggagat gaaagcaacc ctggaatcca aaggggaagt ggagttttac 1620gtgtgtaccc taaagaaaag cgtgaacatc aagaaaaaca tggtgtctat atcaaaggag 1680aagaagaaag agcaccagcg ggttttcact ccatcagtga ttgaaccatc gtttgggatt 1740ggtcggatca tatattgtct gtatgagcat tgtttcagca caaggccaag caaagcaggg 1800gatgagcagt tgaacttgtt ccgtttccct cctcttgtag ctcccatcaa gtgcacggtt 1860ttcccgcttg ttcagaacca acaattcgag gaagtagcca aagttatttc caaggaactc 1920gcctctgtcg gtatctccca taagattgac atcactggta catcgatagg gaagagatat 1980gcgagaaccg atgagcttgg agtgccattt gcaataacag tggactcgga tacatcagtg 2040acaatcagag aaagagacag caaagatcaa gtccgagtca ccttgaagga ggcagcttcc 2100gttgtgagct cagtctcaga ggggaaaatg acgtggcaag acgtctgggc aaccttccct 2160caccattctt ctgctgctgc agacgagtag 219051767DNAArabidopsis thaliana 5atgtcttatg atgagcacac atcatcatca ttcacataca taagccaaat gggtgtgtgg 60tggatagtgc tggtggtggc tgtcttgact cacacggcgt ctgccgccgt gagagaatat 120cattgggagg tggagtacaa gtattggtcg ccggactgca aagagggcgc cgttatgacc 180gtcaacggcg agtttcctgg tcccaccata aaagccttcg ccggagacac catcgtcgtc 240aatctcacca acaaactcac caccgaaggc cttgtcatcc attggcatgg aatccgtcag 300ttcggaagtc catgggcaga tggagcagca ggagttactc aatgcgcaat taaccctgga 360gagactttta cctacaattt cactgttgaa aagccgggaa cacatttcta ccatggacac 420tatggcatgc agagatcagc tgggctatac ggatcgttga ttgtggacgt ggctaaagga 480aagagcgaga gattgagata cgatggtgag tttaatctct tactcagtga ctggtggcat 540gaggctattc cctcccaaga actcggtctt tcttccaaac ctatgcgctg gatcggtgaa 600gctcagagca tattgataaa tgggagggga caattcaatt gttcattagc ggcgcaattt 660agcaacaaca catcattacc aatgtgcacg tttaaagaag gtgatcagtg cgccccacag 720atactccacg tggagccgaa caagacttac cgaatcagac tctccagcac caccgctctt 780gcctccctca acttggctgt tcagggacac aagctagtgg tggtcgaagc cgacggcaac 840tacataacgc cgttcacgac cgacgatatc gacatatact ccggcgaaag ctactccgtc 900cttctcacca ccgatcaaga cccttcacaa aactattaca tctccgtcgg cgtccgtggc 960cggaaaccaa acacaactca ggcactcact atattaaatt acgtaactgc ccctgcttca 1020aaactccctt cttctcctcc accggtgact ccacggtggg acgatttcga acgaagcaaa 1080aatttctcta agaagatctt ctcagcgatg ggatcaccat cgccgccgaa gaaatacaga 1140aaacggttga ttcttctcaa cacacagaat ctaatcgacg gatacacgaa atgggcgatc 1200aacaacgtct ctctagtgac tccggcaacg ccgtatctcg gttcggttaa atacaacttg 1260aaactcggat ttaaccggaa atcgccgccg aggagttacc gtatggatta cgatattatg 1320aatccaccgc cgtttcctaa caccactaca ggaaacggaa tatacgtttt cccgtttaat 1380gtaacggtcg acgtgattat ccaaaacgct aacgttttaa aaggtattgt tagtgagatt 1440catccatggc atcttcacgg tcacgatttc tgggttttgg gttacggtga cgggaaattt 1500aaaccgggaa ttgatgagaa gacgtataat ttgaagaatc cgccgttacg gaatacggcg 1560attttgtatc cgtatggatg gacggcgata aggtttgtga cggataatcc aggggtttgg 1620ttttttcatt gtcacattga accgcatttg catatgggaa tgggtgtggt tttcgcggag 1680ggattaaacc gtattggtaa ggtacctgat gaggcgttgg gttgtggttt aaccaagcaa 1740tttctcatga accggaaccg caattag 176761482DNAArabidopsis thaliana 6atggttttgt ctaagacagt ttccgaatct gatgtctcaa tccattcaac ttttgcttct 60cgttacgtcc gcaactctct tccacgattc gaaatgcctg agaactcaat cccaaaagaa 120gcagcttacc aaatcatcaa cgacgagcta atgctcgatg gtaacccaag gctgaaccta 180gcttccttcg tgaccacatg gatggagcca gaatgtgaca agctcatgat ggagtccatc 240aacaagaact acgtcgacat ggacgagtac cctgtcacca ctgagcttca gaaccgatgt 300gttaacatga tagcacgtct cttcaacgcg ccgcttggtg acggtgaagc tgccgttggt 360gttggcaccg tcggatcgtc ggaggcgatt atgttggccg gtttggcttt taagagacaa 420tggcagaata agcgtaaggc ccaagggctt ccttatgata agcccaatat cgtaaccggt 480gctaatgtcc aggtttgctg ggagaaattc gcaaggtatt tcgaagtgga gcttaaggaa 540gtgaacctaa gagaagacta ttacgtgatg gaccctgtaa aggcggtcga aatggtagac 600gaaaacacaa tttgtgtcgc tgccatcctc ggttcaacgt taaccggtga attcgaagac 660gttaagctcc tcaacgacct ccttgtcgag aaaaacaagc aaaccggatg ggacacgcca 720atacacgtgg acgcagcgag tggtgggttt attgctccgt tcttgtatcc ggagctggag 780tgggatttcc ggctaccgtt ggttaagagt attaatgtga gtggtcacaa atacggtttg 840gtttacgccg gtattggttg ggttgtatgg agaaccaaaa ccgatttgcc tgatgaactt 900atcttccata tcaattatct tggcgctgat caaccaacct ttacactcaa cttctccaaa 960ggttcaagtc aagtgattgc tcagtactac cagctgattc gtcttggatt cgagggttat 1020cgcaatgtga tggataattg tcgggaaaac atgatggtac taagacaagg attagagaaa 1080acgggacgtt ttaaaatcgt ctccaaagaa aacggtgttc cgttagtggc gttttctctc 1140aaagatagta gccgccacaa cgagttcgag gtggcccata cactccgtcg cttcggctgg 1200atcgttccgg cctacacgat gcctgcggat gcgcagcatg tcactgtcct tcgagttgtt 1260atccgagaag atttctctcg aaccttagcc gagagattgg tagctgattt cgagaaggtt 1320ctacacgagc tcgatacgct tccggcgagg gttcacgcca agatggctaa tggaaaagtt 1380aacggtgtta agaagacgcc agaggagacg cagagagaag tcacggccta ctggaagaag 1440ttgttggaga ctaagaagac caacaagaac acaatttgct aa 14827849DNAArabidopsis thaliana 7atgggtacta gagctcagca gattccttta cttgaaggtg agactgataa ttacgatggt 60gttactgtaa ccatggtgga acctatggat tctgaggttt ttactgaaag tcttagggct 120tctctttcgc attggagaga agaggggaag aagggaattt ggataaagct gcctcttgga 180ttggctaatc ttgtggaggc tgcagttagt gaaggattta gatatcacca cgcggagcct 240gagtacttga tgcttgtatc ttggatctct gaaactcctg atacaatccc agccaatgct 300tctcatgttg taggtgctgg tgctttggtc atcaacaaaa atactaaaga ggtcctcgtt 360gtccaggaga ggagtgggtt tttcaaagat aaaaatgtgt ggaagctgcc tactggtgtt 420atcaacgagg gcgaggatat atggactgga gtagctaggg aagtggaaga agaaactgga 480attattgcag attttgtcga agtactggct ttcaggcaaa gccacaaagc catcttaaaa 540aagaaaacag atatgttttt cctgtgtgtc ttaagtccgc gctcttacga tattactgaa 600caaaaatctg agatcttgca agctaagtgg atgccgatcc aagagtatgt agaccaacca 660tggaacaaga agaacgagat gttcaagttc atggctaaca tttgccaaaa gaagtgtgag 720gaagaatact tgggattcgc cattgtgcca actaccacat catctggtaa agagagcttt 780atctactgca atgcggatca tgccaagcgc cttaaagtat cgcgtgacca agcctctgct 840tctctctga 84981383DNAArabidopsis thaliana 8atgacagttg ttggagatgt tgctccaatt ccaaggagga acagttctac ttgctctaat 60gatatcgctg ctccgttatt accagagtgt catggagatg aagttgctca tgatgaattc 120aatggagctt cttttagtgg cgcggttttt aacctcgcca cgactataat tggtgctggt 180atcatggctt tgcctgctac aatgaagatt cttggacttg gacttggaat cacgatgatt 240gttgttatgg ctttcttgac tgatgcgtcg attgagttct tgcttaggtt tagtaaagct 300gggaagaatc gatcttacgg tggtttaatg ggtggttctt ttggtaatcc tggaaggatt 360ttgcttcaag tcgcggtttt agttaataac atcggtgttt tgattgtata catgatcatt 420ataggtgatg tgttggctgg aaagacggaa gatggtatcc atcatttcgg tgttctcgaa 480ggatggtttg gtcaccattg gtggaatgga agagctgcta ttcttctgat tactactctt 540ggtgtgtttg ctccattggc ttgcttcaag cgaatcgatt ctttgaaatt tacatcggcc 600ttatccgtgg ctctggcggt tgtgtttctc attattactg cgggaatttc aattatgaag 660ttgatcagtg gcggtgtggc gatgccaaga ttgttaccag atgttactga cttaacatct 720ttctggaatc tcttcacagt cgtacctgtt cttgtcacag cattcatttg ccattacaat 780gttcacagta tacagaacga gctcgaagac ccctctcaga taagacctgt tgttcgatca 840gcacttatgc tctgctcatc tgtttacata atgacaagta ttttcgggtt cctcttgttt 900ggtgatgata ctcttgatga tgtccttgca aactttgaca ctgatcttgg aatccctttt 960ggttctatcc ttaatgatgc ggttcgagtt agctacgcgc ttcatttgat gctcgtgttc 1020ccgattgttt tctaccctct tcggattaac attgacgggc tcttgttccc ttccgctcga 1080tcattatcta cctcaaatgt gaggttcggt tgcctcactg ccggtctcat ctctgtaatc 1140ttcttgggtg caaacttcat cccaagcatt tgggatgctt tccaattcac tggagcaacc 1200gccgctgttt gtctcggctt catcttccca gcttctatca tactaaagga tcgtcatgac 1260aaagcaacaa acagggacac aaccttagct attttcatga ttgttcttgc ggtattgtcc 1320aatgcaatcg ccatttacag cgatgcttat gcgctattca agaagaacgc tcctcgtgag 1380taa 13839402DNAArtificial SequenceArabidopsis thaliana putative AP2/EREBP transcription factor ; with A33V mutation 9atggatcaag gaggtcgtag cagtggtagt ggaggaggag gagccgagca agggaagtac 60cgtggagtaa ggagacgacc ttggggtaaa tacgccgtgg aaataagaga ttcgaggaag 120cacggagagc gtgtgtggct agggacattc gacactgcgg aagacgcggc tcgagcctat 180gaccgagccg cctattcaat gagaggcaaa gctgccattc tcaacttccc tcacgagtat 240aacatgggaa ccggatcctc atccactgcg gctaattctt cttcctcgtc gcagcaagtt 300tttgagtttg agtacttgga cgatagcgtt ttggatgaac ttcttgaata tggagagaac 360tataacaaga ctcataatat caacatgggc aagaggcaat aa 402101074DNAArabidopsis thaliana 10atggtaatat atcatcgcaa agtagtcttt acgtacgtaa gagcaaagcg tttctatcat 60ttccttaata ttgaaatggt gacagacttc aaatccctac taccagttat cgacatcagt 120cctttactgg ccaaatgtga tgatttcgac atggctgagg atgcgggggt tgtggaagta 180gtcggaaaac tggacagggc atgtcgggac gtcggattct tctacgtgat tggtcatgga 240atatcggatg atcttataaa taaggtgaaa gagatgacgc atcagttctt cgagcttcct 300tacgaggaga aacttaagat caagattact ccaactgctg gatacagagg ataccaaaga 360attggagtaa attttacgag tgggaaacaa gatatgcacg aagccattga ttgttacaga 420gagttcaagc aagggaaaca tggggacatc ggaaaggtct tggaagggcc aaaccagtgg 480ccaggaaatc ctcaagagta caaagattta atggagaagt atattaagct gtgcacagat 540ctttctagaa atatcttaag gggaatctca ttagcccttg gtggatcacc ttatgagttt 600gagggaaaga tgcttagaga ccctttttgg gtaatgcgta tcattggtta tccaggtgta 660aaccaagaaa atgttattgg atgtggagct cacactgact atggcttgtt gacgcttata 720aatcaagatg atgataaaac tgctcttcag gtgaaaaacg tggacggtga ttggatacca 780gctattccga tccctggatc atttatctgc aacatcggtg acatgttaac gatactatca 840aacggagttt accaatccac actacataaa gtaatcaata actctcctaa ataccgtgta 900tgtgttgcat ttttctacga gactaacttc gaggcggagg tagagccact tgatatcttt 960aaagagaagc atccacgaaa agaaacatct caagttgcca aaagagtcgt ctatggacag 1020catctgatta acaaagtcct gacaaccttt gcaaatttag tggaaaacag ttaa 1074111116DNAArabidopsis thaliana 11atgcctgtac ttttctcctc tcgttctctc atactctcca ttattgtccc cttacttata 60tctatcgctc tctacaaact cgacacattc gaccccgcta ttgttccttc cgatgcattc 120acatcctctg ctacttcact cccgccgctt ataaacgacg aatttctcac cggagctgag 180tttatcggtg tcggtcttct caatataccc gaagatatcg cctaccataa ggaatctaat 240ctcatctaca ctggttgtgt tgatggatgg gtgaaacgag tcaaggtcgc cgactcggtt 300aatgactcag tcgttgagga ttgggtcaat actggtggta gaccgctcgg aatcgcgttc 360ggaatccacg gcgaagtcat tgtcgcagac gtacacaagg gactgttgaa tataagcggt 420gacggaaaga agacggaatt gttgacggac gaagcggacg gtgtgaagtt taagctgacg 480gatgcagtta ccgttgcaga taacggcgtt ttgtatttca cagatgcttc ttacaagtac 540actctcaatc aattgagttt agacatgtta gaagggaaac cttttggacg actcttgagc 600tttgatccca ccacacgtgt

tacaaaagta cttctcaaag acctctactt cgctaatggc 660atcaccatct ctcctgatca aactcatctc atcttctgtg aaactccaat gaaaaggtgc 720agcaagtatt acatcagtga ggagcgtgtg gaggtattca ctcaaagctt accaggttat 780ccagataaca ttcgttacga tggagatgga cattactgga ttgcattgcc ttcgggagtc 840acgacattgt ggaatatctc gttgaaatac cctttcttga ggaaactcac agcaatggtg 900gctaagtacg gcgtggatct tatgtttatg gagaatgcag gcgttttaca ggtggatttg 960gatggaaatc ccatcgcgta ctaccatgat ccaaaactct ctcacatagc cacatgtgac 1020aagattggga aatatctcta ttgtggaagt ctctcgcaat cacatatcct ccgacttgat 1080ctcctgaaat atcctgcaca gaacaagaaa ctttga 1116121407DNAArabidopsis thaliana 12atgggtcgtg tccgaaaatc agatttcggt tccattgttc ttgttctgtg ttgtgtccta 60aatagcttac tctgtaatgg aggcatcacc agtagatatg tcagaaaatt agaagcaacc 120gttgatatgc ctcttgatag cgatgttttt cgtgttcctt gtggctacaa tgctcctcaa 180caggtgcata taacacaagg agatgtagaa ggaaaggcag tgatagtatc atgggtgaca 240caagaagcaa aaggatctaa caaagtcatt tactggaaag agaatagcac caaaaagcac 300aaagctcatg gcaaaaccaa tacttacaag ttctacaatt atacttctgg ttttatccat 360cattgcccta tcagaaactt agagtatgac accaagtact attatgtgtt aggtgtggga 420caaacggagc gtaagttttg gttcttcact cctcctgaga tcggtcctga tgttccctac 480acttttggtc tcattgggga tcttgggcag agttatgact caaacataac cttaacacat 540tatgagaata acccaacaaa agggcaagcg gttttgttcg tcggcgatat ctcatacgct 600gatacttatc cggatcatga caataggaga tgggacagtt ggggaagatt tgctgaaaga 660agcacagctt atcagccttg gatttggact accggaaacc atgaactcga ttttgccccg 720gagattggtg aaaacagacc gtttaagccg ttcacgcata ggtaccgaac tccttaccga 780tcatcaggca gcaccgaacc attctggtac tcgataaaga gaggaccggc ttacataatc 840gtgctagctt catattcagc atatggaaaa tacacaccac agtaccaatg gctcgaagag 900gagttcccaa aggttaacag aacggaaact ccgtggttga ttgttctgat gcattcacca 960tggtacaaca gctatgatta ccattacatg gaaggtgaaa caatgagggt aatgtatgag 1020gcttggtttg tcaagtacaa agtagatgta gtttttgcag gtcatgtcca cgcctacgaa 1080agatcggaac gtgtatcaaa catagcttac aatgttgtta acggcatttg cactccagtc 1140aaagatcaat cagctccagt ctatatcacc attggtgatg gaggcaatat tgagggcttg 1200gctaccaaaa tgactgagcc tcagcctaag tactctgcct ttagagaagc aagcttcggg 1260catgctatat tctcgataaa gaacaggacg catgctcact atggatggca taggaaccat 1320gatggttatg ctgtggaggg tgacagaatg tggttttata acagattttg gcatcctgtt 1380gatgattctc cttcttgtaa ttcttga 1407131500DNAArabidopsis thaliana 13atgttattga aacccggaaa taagcttgtc tcgccggaga cgagtcatca tcgtgactct 60gcctcaaact catcaaacca taaatgtcaa caacaaaaac cgcgtaaaga taagcagaag 120caagttgagc aaaacacaaa gaagattgaa gaacatcaaa taaaatcaga gtcgactctg 180ttaatcagca atcacaacgt taacatgagc tcccaaagca acaacagcga aagcacatcc 240actaataatt cctctaagcc tcacacggga ggagatatta ggtgggacgc agtgaattcc 300ttgaaatcta gaggaatcaa gcttggaatc agcgattttc gtgttttgaa acggctaggc 360tacggagata tcggtagtgt ttatcttgtt gaactcaaag gggcaaatcc cacgacgtat 420ttcgcgatga aagtgatgga caaggcttct cttgtgagca ggaacaagct gttgagagct 480cagactgaaa gagagatctt gtctcagctt gatcatcctt tcttgcctac tctttactct 540cacttcgaaa ctgataaatt ctattgcttg gtgatggagt tttgtagtgg tggaaatctc 600tattccttga gacagaagca acccaacaag tgtttcacag aagacgctgc aaggttcttt 660gcatcagaag tgttattagc attagagtac ttacatatgc tcggaatcgt ataccgagat 720ttaaagccag agaacgttct ggttcgagac gacggtcaca ttatgctctc cgatttcgat 780ttatccctcc gatgttcagt caacccaaca cttgtcaaat ctttcaacgg cggtggaacc 840accggaatca ttgacgacaa tgcggcggta caaggatgtt accaaccatc cgcattcttc 900ccacgaatgc tccaatcctc gaagaaaaac cgtaaatcca aatccgattt cgatggatcg 960ttaccagaac tcatggcaga gccgacaaac gtgaaatcaa tgtcttttgt cggaacacac 1020gagtatttag cgccggagat tatcaaaaac gaaggtcacg gaagcgccgt cgattggtgg 1080acgttcggga tattcatcta cgagcttctt catggagcaa caccatttaa aggacaagga 1140aacaaagcca cgctttataa tgtaatcgga caacctctta gattcccgga atattctcag 1200gttagctcga cggcgaagga tttgatcaaa ggtttattgg taaaagagcc gcaaaacaga 1260attgcgtata agcgaggcgc gacggagatc aagcaacatc cattttttga aggtgtgaat 1320tgggcgttga ttcgtggaga aacaccgcca catttgccgg agccggtgga tttctcgtgt 1380tatgtgaaga aggagaagga gtctttgccg ccggcggcaa cggaaaagaa gagcaagatg 1440tttgatgaag ctaataagag cggtagtgat cctgattaca ttgtttttga atatttttag 1500144659DNAChlamydomonas reinhardtii 14atggaggcgg tcgcgaccgt tgtggccaac gagcccaagg ttgctgatat ggaggagatt 60ctcgccgagc gcgatgcctg cggcgtgggc ttcatcgcga acctgaagaa cgttcagagc 120cacaccgtcg tgaagcaggc tctgaccgct ctgggctgca tggagcaccg cggtgcttgc 180tctgcggacg atgactcggg agatggtgcg ggtcttatga ctcaaatccc ctggaagctg 240ctgaagaagg agatgcccgc gctgaacgag acgaccacgg gtgtcggcat ggtgttcatg 300cccaatgatg acgctctaga ggcgcagtgc aagcagatcc tggagcaggt ctgcgccaag 360gagggcgtga aggtggtggg ctggcgcaag gtgcccgtca accacgacat cgtgggccgc 420ttcgccaaag tgacggagcc gcgcatctgg caggtcctga ttgagggcaa gtccggtcag 480gtcggcgacg agctggagcg tgagctgttc ctggtgcgca agctggtgga gaaggctaag 540aacgccgcgc tgcccgctga gttcgctccc gacttctaca tttgcacgct gtccagccgc 600accattgtgt acaagggcat gctgcgctcg gcggtcgtgg gcaccttctt ccgcgacctg 660gaaaaccccg acttcgagtc ggcgttcgcc atctaccacc gccgcttctc caccaacacc 720acccccaagt ggccactggc gcagcccatg cgcgtgctgg gccacaacgg cgagatcaac 780accctgcagg gcaacctgaa ctgggttgcc tcgcgcgagc acgagctgag caaccccatc 840tggaagggcc gcgaggcgga gctgacgccg ctgtgcaacg ccgcgcagtc ggacagcgcc 900aacctggaca acgtggcgga gctgctggtg cgcaccggca ccgacccgca ggacgcgctc 960atgctgctgg tgccggaggc ctaccgcaac caccccgacc tcatgaagga gtaccccgag 1020gtggtggact tctacgagtt ctacgagggc ctgcaggagg gctgggacgg ccccgcgctg 1080ctggtgttct ccgacggcaa gcgcgtgggc gcccgcctgg accgcaacgg cctgcgcccc 1140gcgcgcttct ggcagaccaa ggacgacatg atttacgtgg cctccgaggt gggcgtgctg 1200ggtgacgcca tcaccaacgc cgagaacatc gtggccaagg gccgcctggg ccccggccag 1260atggtgtgcg ccgatctgga gaagggcatt ttctcggaga cgtcggccat cagcaagctg 1320gtggctggcc gcaagcccta caaggagtgg ctggccgcct cgctgcgccg cctgacggac 1380ctgggcgaga gcaccttcct gaacgagccc atgtacgacg ccgctaccat gctgcgcctg 1440cagtccgcca tcggcatgga cgccgagaac gcgcagatgg tggtggagag ccaggcgcag 1500accggcgtgg agcccaccta ctgcatgggt gacgacatcc cgctggccgt gctgtcggac 1560aagccgcaca tgctgtacga ctacttcaag cagcgcttcg cgcaggtgac caacccgccc 1620atcgacccgc tgcgtgaggg cctggtcatg agcctggaga tgcgcctggg cgcgcgcggc 1680aacctgctga accccggcgc cgacagctac aagcaggtgc tactggactc gcccatcctg 1740ctggagagcg agatgcaggc catctccacc gacaaggtgc tgggctccaa gaccttcaag 1800ctgttcttcg aggccggcaa gcccggcgcc atggaggcgg cgctgaagaa gctgtgctcg 1860gacgtggagg ccgccgtcaa ggccggctgc cagtgcgtgg tgctgtcgga ccgccccgac 1920ggcggtatgg acgccggtaa ggcgcccatc cccgcgctgc tggccaccgg cgccgtgcac 1980caccacctga tccgcacctc gctgcgcagc gacacctcga tcgtggtgga caccgccacc 2040tgctacagca cccaccacgc cgccatgctg atcggcttcg gcgcgcacgc catctgcccc 2100tacctgggct acgagaccag ccgccagtgg cgcctgtcgg cgcgcacgca gagcctgatc 2160aaggccggca aggtgccgga catcagcgtc aaggtggcgc agaagaactt caagaagtcg 2220ctggagaagg gcgtgctcaa gatcctgtcc aagatgggca tctcgctgct gtcgtgctac 2280cacggcgcgc agatcttcga ggcgtacggc ctgggcaagg acgtgatgga catgtgcttc 2340aagggcaccg tgtcgcgcat tggcggcatg agcctggcgg acctgcagcg cgagtccgag 2400agcctgtggg ccaagggctt ccccgagaag gccatgacca agctggagga ctacggcttc 2460atccagtcca agcccaaggg tgagttccac tccaacaacc agaccatggc caagctgctg 2520cacaaggcca tcggcctggg caacggctcc gctgccgaca aggacgccta caaggcctac 2580cagcagcact tcgcggactc gcccgtggcc gtcctgcgcg actgcctgga gttcaagtcg 2640gaccgcggcc ccatctccat tgaccaggtg gagcccgccg ccgccatcat ggagcgcttc 2700tgcaccggtg gcatgtcgct gggcgccatc tcgcgcgaga cgcacgagac catcgccatc 2760gccatgaacc gcatcggcgg caagtccaac tcgggcgagg gcggcgagga cccgatccgc 2820tggctgcacc tgtcggacgt ggacggcgag ggcaagtccg ccaccgcctc ctacctgcgc 2880ggcctgcgca acggcgacac cgccacctcc aagatcaagc aggtggcgtc gggccgcttc 2940ggcgtcacgc ccgagtacat catgaacgcg gagcagatgg agattaagat tgcgcagggc 3000gccaagcccg gtgagggcgg ccagctgccc ggccagaagg tgtcgcccta catcgcgcag 3060ctgcgccgct ccaagcccgg cgtgccgctc atctcgcccc ctccccacca cgacatctac 3120tccattgagg acctggcgca gctcatctac gacctgcacc aggtcaaccc ccgcgccaag 3180gtgtcggtca agctggtggc cgaggccggc atcggcgtcg tcgccagcgg cgtggccaag 3240gccaacgccg acatcatcca ggtgtctggc cacgacggcg gcaccggcgc ctcgcccatc 3300tcgtccatca agcacgcggg cggccccatg gagatgggcc tggcggagac gcaccagacg 3360ctggtgcgca acgagctgcg cgagcgcgtg gtgctgcgcg tggacggcgg cgtgcgcaac 3420ggccgcgacg tgctgatggg cgcgctgatg ggcgccgacg agttcggctt cggcaccgtg 3480gccatgattg ccaccggctg catcatggcg cgcgtgtgcc acaccaacaa ctgccccgtg 3540ggcgtggcgt cgcagcgcga ggagctgcgc gcgcgcttcc ctggcgcccc cgaggacctg 3600gtcaactact tccacttcgt tgccgaggag gtccgtgccg agctggccaa catgggctac 3660cgcagcctgg atgaggtcat tggccgcgcc gacctgctga agcagcgctc cgtcaagctg 3720gccaagaccg agggcctgga cctgtccttc ctcaccacct tcgccggcgc cagcggcaag 3780tcctcgaccc gccgcgcgca ggaggtgcac gacaacggcc cgcagctgga cgaccgcatc 3840ctggcggaac ccgaggtgat ggccgccatc aaggaccaca agaccgtgtc caaggccttc 3900gagatcgtca acgtggaccg ctcctcgcta ggccgcgttg ccggtgtcat cgccaagcac 3960cacggcgaca gcggcttcca gggcaaggtc aagctgacgc tgaccggctc gggcggccag 4020tcgttcggct gcttctgcgt caagggcctg gaggtgaagc tggtgggtga ggccaacgac 4080tacgtgggca agggcatgaa cggcggcgag atcgccatcg tgccgcccgc caactcgccc 4140ttcaagcccg aggaggcctc gctggtcggc aacacttgcc tgtacggcgc caccggcggc 4200cggctgttcg tgaacggccg cgccggcgag cgcttcgcgg tgcgcaactc gctagcggag 4260gcggtggtgg agggcgcggg cgaccactgc tgcgagtaca tgactggcgg ctgcgtgatc 4320gtgctgggct cggtgggccg caacgtggcg gcgggcatga cgggcggcct gggctacttc 4380ctggatgagg acggctcgtt cacggacaag gtcaacacgg agatcgttag cgtgcagcgc 4440gtgatcacca aggccggaga ggcgcagctg cgggggctgc tggaggcgca cgtggcgcac 4500actggcagcg ccaaggccaa gagcctgctg gccaactggg aggcgtcgct gggcaagttc 4560tggcagctgg tgccgcccgc ggagaagaac accgccgagg tcaacccctc ggtggcgcag 4620ccggcggcgg ctggcgccaa ggtggctgtc agcgcatga 4659151350DNAArtificial SequenceChlorella sorokiniana NADP-specific glutamate dehydrogenase (NADP-GDH), N terminus residues 1 to 74 truncated 15atgcacggca tcaagaaccc cgagctgcgc cagctgctga ccgagatctt catgaaggac 60ccggagcagc aggagttcat gcaggcggtg cgcgaggtgg ccgtctccct gcagcccgtg 120ttcgagaagc gccccgagct gctgcccatc ttcaagcaga tcgttgagcc tgagcgcgtg 180atcaccttcc gcgtgtcctg gctggacgac gccggcaacc tgcaggtcaa ccgcggcttc 240cgcgtgcagt actcgtccgc catcggcccc tacaagggcg gcctgcgctt ccacccctcc 300gtgaacctgt ccatcatgaa gttccttgcc tttgagcaga tcttcaagaa cagcctgacc 360accctgccca tgggcggcgg caagggcggc tccgacttcg accccaaggg caagagcgac 420gcggaggtga tgcgcttctg ccagtccttc atgaccgagc tgcagcgcca catcagctac 480gtgcaggacg tgcccgccgg cgacatcggc gtgggcgcgc gcgagattgg ctaccttttc 540ggccagtaca agcgcatcac caagaactac accggcgtgc tgaccggcaa gggccaggag 600tatggcggct ccgagatccg ccccgaggcc accggctacg gcgccgtgct gtttgtggag 660aacgtgctga aggacaaggg cgagagcctc aagggcaagc gctgcctggt gtctggcgcg 720ggcaacgtgg cccagtactg cgcggagctg ctgctggaga agggcgccat cgtgctgtcg 780ctgtccgact cccagggcta cgtgtacgag cccaacggct tcacgcgcga gcagctgcag 840gcggtgcagg acatgaagaa gaagaacaac agcgcccgca tctccgagta caagagcgac 900accgccgtgt atgtgggcga ccgccgcaag ccttgggagc tggactgcca ggtggacatc 960gccttcccct gcgccaccca gaacgagatc gatgagcacg acgccgagct gctgatcaag 1020cacggctgcc agtacgtggt ggagggcgcc aacatgccct ccaccaacga ggccatccac 1080aagtacaaca aggccggcat catctactgc cccggcaagg cggccaacgc cggcggcgtg 1140gcggtcagcg gcctggagat gacccagaac cgcatgagcc tgaactggac tcgcgaggag 1200gttcgcgaca agctggagcg catcatgaag gacatctacg actccgccat gggcgcgtcc 1260cgcgagtaca atgttgacct ggctgcgggc gccaacatcg cgggcttcac caaggtggct 1320gatgccgtca aggcccaggg cgctgtttag 1350161344DNAEscherichia coli 16atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat 60caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa 120caaaatccaa aatatcgcca gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg 180atccagtttc gcgtggtatg ggttgatgat cgcaaccaga tacaggtcaa ccgtgcatgg 240cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg gtatgcgctt ccatccgtca 300gttaaccttt ccattctcaa attcctcggc tttgaacaaa ccttcaaaaa tgccctgact 360actctgccga tgggcggtgg taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa 420ggtgaagtga tgcgtttttg ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg 480gataccgacg ttccggcagg tgatatcggg gttggtggtc gtgaagtcgg ctttatggcg 540gggatgatga aaaagctctc caacaatacc gcctgcgtct tcaccggtaa gggcctttca 600tttggcggca gtcttattcg cccggaagct accggctacg gtctggttta tttcacagaa 660gcaatgctaa aacgccacgg tatgggtttt gaagggatgc gcgtttccgt ttctggctcc 720ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact 780gcgtcagact ccagcggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggca 840cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg cagattacgc caaagaattt 900ggtctggtct atctcgaagg ccaacagccg tggtctctac cggttgatat cgccctgcct 960tgcgccaccc agaatgaact ggatgttgac gccgcgcatc agcttatcgc taatggcgtt 1020aaagccgtcg ccgaaggggc aaatatgccg accaccatcg aagcgactga actgttccag 1080caggcaggcg tactatttgc accgggtaaa gcggctaatg ctggtggcgt cgctacatcg 1140ggcctggaaa tggcacaaaa cgctgcgcgc ctgggctgga aagccgagaa agttgacgca 1200cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt 1260gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg 1320atgctggcgc agggtgtgat ttaa 134417861DNAArtificial SequenceHomeobox fusion protein, Arabidopsis HB-17 N terminus residues 1 to 91 fused to soybean HB-17 C terminus residues 20-213 17atgataaaac tactatttac gtacatatgc acatacacat ataaactata tgctctatat 60catatggatt acgcatgcgt gtgtatgtat aaatataaag gcatcgtcac gcttcaagtt 120tgtctctttt atattaaact gagagttttc ctctcaaact ttaccttttc ttcttcgatc 180ctagctctta agaaccctaa taattcattg atcaaaataa tggcgatttt gccggaaaac 240tcttcaaact tggatcttac tatctccgtt ccaggttttg cttcttcacc aacccttctt 300ccctcatcat ctgtgaaaga attggacata aatcaagtac ctcttgaaga agattggatg 360gcatcaaaca tggaagatga agaagaaagc agcaatggag aacctcctcg aaagaaactc 420cgtctcacaa aggaacaatc tcttctcctt gaagaaagct ttagacaaaa ccacacgttg 480aacccaaagc agaaagagtc tttggcaatg caactgaagc tgcgaccaag gcaagtggag 540gtgtggtttc agaaccgtag ggccaggagc aagctgaagc agacagagat ggagtgcgag 600tacctcaaga ggtggttcgg ttccctcaca gagcagaacc ggaggctcca gagggaagtg 660gaggagctgc gagccattaa ggtgggccca cccaccgtga tctcccctca ctcctgcgaa 720ccgctcccgg cctccacact ttccatgtgt ccccgctgcg agcgtgtcac ctccaccgcc 780gacaaaccgc cctccgccgc ggccactttg tccgctaaag tgccgccaac tcaatcccgc 840caaccctccg cggcctgtta g 861181038DNAGlycine max 18atgtgtggag gagctatcat ctctgatttc attccggcgg cggcgatcgc cgggtctcgc 60cgcctgaccg ccgattacct gtggccggat ttgaagaagc ggaagtctga cttggacgtt 120gacttcgagg ctgatttcag ggattttaaa gacgattctg atatcgacga cgacgacgac 180gatcaccaag tcaagccctt tgctttcgcc gcctcttctc gtctgtctac ggcagcgaaa 240tctgtggcat tccaaggtcg ggctgagata tctgcaaata gaaagaggaa gaatcagtat 300aggggaatcc gtcaacgccc ttggggaaaa tgggcagctg agattcggga tccaagaaag 360ggggttcgtg tctggcttgg aaccttcaac actgctgaag aagctgcaag agcttatgat 420gctgaagcac ggaggattcg tggcaagaaa gccaaggtga attttcctga ggcaccaggt 480acttcttctg taaaacgttc caaggtaaat ccacaggaaa atcttaagac tgttcagccc 540aatctgggtc acaagttcag tgctggcaac aatcacatgg atctggtgga acagaaaccc 600ctagttagcc agtatgctaa catggcttcc ttccctggca gtggaaatgg gctaagatcc 660cttccttcgt ctgatgatgc aaccctttac ttcagttcag atcaagggag taattcattt 720gattatgctc ctgagatctc atccatgctt tctgctcctt tggattgtga atctcatttt 780gtgcaaaatg ccaaccagca gcagcctaac tctcagaatg tggtatctat tgaagatgat 840tctgcaaaga cactctctga ggagcttgtg gatattgaat ctgagttgaa gttctttcag 900atgccttatc ttgaagggag ctggggcgat acttcattgg aatccttgct atctggtgac 960acaactcagg atggtggaaa cctcatgaac ctttggtgct ttgatgacat tccttccatg 1020gctggtggag ttttctga 103819774DNAOryza sativa 19atgtcgatgg gaccagcagc cggagaagga tgtggcctgt gcggcgccga cggtggcggc 60tgttgctccc gccatcgcca cgatgatgat ggattcccct tcgtcttccc gccgagtgcg 120tgccagggga tcggcgcccc ggcgccaccg gtgcacgagt tccagttctt cggcaacgac 180ggcggcggcg acgacggcga gagcgtggcc tggctgttcg atgactaccc gccgccgtcg 240cccgttgctg ccgccgccgg gatgcatcat cggcagccgc cgtacgacgg cgtcgtggcg 300ccgccgtcgc tgttcaggag gaacaccggc gccggcgggc tcacgttcga cgtctccctc 360ggcggacggc ccgacctgga cgccgggctc ggcctcggcg gcggcagcgg ccggcacgcc 420gaggccgcgg ccagcgccac catcatgtca tattgtggga gcacgttcac tgacgcagcg 480agctcgatgc ccaaggagat ggtggccgcc atggccgatg ttggggagag cttgaaccca 540aacacggtgg ttggcgcaat ggtggagagg gaggccaagc tgatgaggta caaggagaag 600aggaagaaga ggtgctacga gaagcaaatc cggtacgcgt ccagaaaagc ctatgccgag 660atgaggcccc gagtgagagg tcgcttcgcc aaagaagctg atcaggaagc tgtcgcaccg 720ccatccacct atgtcgatcc tagtaggctt gagcttggac aatggttcag atag 774202916DNAZea mays 20atgatcgtgc aacccatcga gctacgcgcg tggactgcct tccctgggtc ggcgcaggag 60gggatcggaa ggatggcggc gtcggtttcc agggccatct gcgttcagaa gccgggctca 120aaatgcacca gggacaggga agcgacctcc ttcgcccgcc gatcggtcgc agcgccgagg 180cccccgcacg ccaaagccgc cggcgtcatc cgctccgact ccggcgcggg acggggccag 240cattgctcgc cgctgagggc cgtcgttgac gccgcgccga tacagacgac caaaaagagg 300gtgttccact tcggcaaggg caagagcgag ggcaacaaga ccatgaagga actgctgggc 360ggcaagggcg cgaacctggc ggagatggcg agcatcgggc tgtcggtgcc gccggggttc 420acggtgtcga cggaggcgtg ccagcagtac caggacgccg ggtgcgccct ccccgcgggg 480ctctgggccg agatcgtcga cggcctgcag tgggtggagg agtacatggg cgccaccctg 540ggcgatccgc agcgcccgct cctgctctcc gtccgctccg gcgccgccgt gtccatgccc 600ggcatgatgg acacggtgct caacctgggg ctcaacgacg aagtggccgc cgggctggcg 660gccaagagcg gggagcgctt cgcctacgac tccttccgcc gcttcctcga catgttcggc 720aacgtcgtca tggacatccc ccgctcactg ttcgaagaga agcttgagca catgaaggaa 780tccaaggggc tgaagaacga caccgacctc acggcctctg acctcaaaga gctcgtgggt 840cagtacaagg aggtctacct ctcagccaag ggagagccat tcccctcaga ccccaagaag 900cagctggagc tagcagtgct ggctgtgttc aactcgtggg

agagccccag ggccaagaag 960tacaggagca tcaaccagat cactggcctc aggggcaccg ccgtgaacgt gcagtgcatg 1020gtgttcggca acatggggaa cacttctggc accggcgtgc tcttcactag gaaccccaac 1080accggagaga agaagctgta tggcgagttc ctggtgaacg ctcagggtga ggatgtggtt 1140gccggaataa gaaccccaga ggaccttgac gccatgaaga acctcatgcc acaggcctac 1200gacgagcttg ttgagaactg caacatcctg gagagccact ataaggaaat gcaggatatc 1260gagttcactg tccaggaaaa caggctgtgg atgttgcagt gcaggacagg gaaacgtacg 1320ggcaaaagtg ccgtgaagat cgccgtggac atggttaacg agggccttgt tgagccccgc 1380tcagcgatca agatggtaga gccaggccac ctggaccagc ttctccatcc tcagtttgag 1440aacccgtcgg cgtacaagga tcaagtcatt gccactggtc tgccagcctc acctggggct 1500gctgtgggcc aggttgtgtt cactgctgag gatgctgaag catggcattc ccaagggaaa 1560gctgctattc tggtaagggc ggagaccagc cctgaggacg ttggtggcat gcacgctgct 1620gtggggattc ttacagagag gggtggcatg acttcccacg ctgctgtggt cgcacgtggg 1680tgggggaaat gctgcgtctc gggatgctca ggcattcgcg taaacgatgc ggagaagctc 1740gtgacgatcg gaggccatgt gctgcgcgaa ggtgagtggc tgtcgctgaa tgggtcgact 1800ggtgaggtga tccttggaaa gcagccgctt tccccaccag cccttagtgg cgatctggga 1860actttcatgg cctgggtgga tgatgttaga aagctcaagg tcctggctaa cgccgatacc 1920cctgatgatg cattgactgc gcgaaacaat ggggcacaag gaattggatt atgccggaca 1980gagcacatgt tctttgcttc agacgagagg attaaggctg tcaggcagat gattatggct 2040cccacgcttg agctgaggca gcaggcgctc gaccgtctct tgccgtatca gaggtctgac 2100ttcgaaggca ttttccgtgc tatggatgga ctcccggtga ccatccgact cctggaccct 2160cccctccacg agttccttcc agaagggaac atcgaggaca ttgtaagtga attatgtgct 2220gagacgggag ccaaccagga ggatgccctc gcgcgaattg aaaagctttc agaagtaaac 2280ccgatgcttg gcttccgtgg gtgcaggctt ggtatatcgt accctgaatt gacagagatg 2340caagcccggg ccatttttga agctgctata gcaatgacca accagggtgt tcaagtgttc 2400ccagagataa tggttcctct tgttggaaca ccacaggaac tggggcatca agtgactctt 2460atccgccaag ttgctgagaa agtgttcgcc aatgtgggca agactatcgg gtacaaagtt 2520ggaacaatga ttgagatccc cagggcagct ctggtggctg atgagatagc ggagcaggct 2580gaattcttct ccttcggaac gaacgacctg acgcagatga cctttgggta cagcagggat 2640gatgtgggga agttcattcc cgtctatcct gctcagggca tcctccaaca tgaccccttc 2700gaggtcctgg accagagggg agtgggcgag ctggtgaagt ttgctacaga gaggggccgc 2760aaagctaggc ctaacttgaa ggtgggcatt tgtggagaac acggtggaga gccttcctct 2820gtggccttct tcgcgaaggc tgggctggat tacgtttctt gctccccttt cagggttccg 2880attgctaggc tagctgcagc tcaggtgctt gtctag 291621792DNAZea mays 21atgttggagt tgcgtctggt gcaggggagc ctcctcaaga aggtcttgga ggcgatccgc 60gagctggtca cggacgccaa cttcgactgc tccgggaccg ggttctcgct gcaggccatg 120gactcgagcc acgtcgcgct cgtcgcgctg ctccttcgcg ccgagggctt cgagcactac 180cgctgcgacc gcaacctctc catgggcatg aacctcaaca acatggccaa gatgctccgc 240tgcgctggca acgaggacat catcaccatc aaggccgacg acggctccga caccgtcact 300ttcatgttcg agtcgcccaa gcaagataag atcgcggatt tcgagatgaa actgatggac 360attgatagcg agcacctcgg aatcccggat tccgagtacc aggccatcgt ccgcatgcct 420tctgctgagt ttatgaggat ctgcaaagat cttagcagca tcggagacac agtcgtcatc 480tcggtgacta aggagggcgt gaagttctcc acatctggag aaattgggag tgcaaacatt 540gtctgcaggc agaaccaaac tattgacaag ccagaagagg ctaccattat agagatgcag 600gagccggttt ccctgacctt tgccctgcgg tacatgaact ccttcaccaa ggcgtcttca 660ctgtctgagc aagtcactat cagcctgtcg tccgagcttc cagtggtggt cgagtacaag 720atcgctgaga tgggttacat tagattttac ctggccccca agatcgacga tgacgaggag 780atgaagccct ag 792222670DNAZea mays 22atggcgaagt cgagcgcgga cgacgccgag ctgcggcgcg cgtgcgccgc ggccgtggcg 60gcgtcaggcg cgcgcgggga ggaggtggcc ttctccatcc gcgtcgccaa gggccgcggc 120atcttcgaga agctcggcag gctcgccaag ccccgtgtcc tcgcgctcac cgttaaacaa 180tcatcaagag gcgaggcgaa caaagctttt ctccgagtat taaaatattc atctggggca 240gtgctcgagc cagccaaact ttacaagctg aaacatctaa caaaggttga ggttatttcc 300aatgatccca gtggttgtac atttgttctg ggatttgata accttaggag tcagagtgtt 360gccccgcctc aatggacaat gcgtaacatt gatgacagaa atcgtctact tttttgtatt 420ctgaatatgt gcaaggagat actcagttat cttccaaaag ttgttggaat tgatattgtg 480gagctagctc tttgggcgaa ggaaaacaca ctgaccatag ataatcaagt gagtacccaa 540gatggtcaag aaacatcagt tgctactcaa accgagagga aagtaacagt aactgttgaa 600aatgatctcg tgtcccaagc aaaagaggag gaagaagaca tggaggcact tcttgacacg 660tatgttatgg gaataggtga agcagatgca ttctctgaga gattgaagca ggagcttgtg 720gctttggagg ctgcaaatgt atatcaatta ctggaaagcg agcctttaat agaagaggtc 780ttgcagggtc tggatgctgc tagtgcaact gtagatgata tggatgagtg gttacggatt 840ttcaatctga agctgaggca catgagagaa gatattgcat cgattgaatc acgtaacaat 900ggcttggaga tgcagtctgt aaacaacaaa gggcttatgg aagagctaga taaattgctt 960gagcgtctgc ggattccaca agagtttgca gcatcattaa ctggaggctc atttgaagaa 1020tcacggatgc tgaaaaatgt tgaggcatgt gaatggttga caggggccat tcgcagtctt 1080gaagttccta atttggaccc atgctatgtc aacatgcgcg ctgttagaga gaaaaaggca 1140gagctggaga aactgaaaac aacttttgtt cgacgagcat cagagttttt gcggaactac 1200ttctccagtt tggtagactt catgatcagt gacaaaagct acttttcaca gcgtggacaa 1260ttgaagcggc ctgatcatgc agaccttagg tataaatgca ggacatatgc ccgacttctg 1320cagcacttaa agagtctgga caagagctgc ttaggtccct taaggaaggc atactgtcat 1380tcccttaatt tactactacg acgagaggca cgtgaatttg ccaatgaact tcgtgcaagt 1440acaaaagcac cgaagaatcc tgctgtttgg cttgaaggtt ctggcggctc tggtcataat 1500ggaagcagtt ctgatacttc acaggtctca gatgcatact caaagatgct tacaatattt 1560atcccgcttc ttgtggatga gagttccttt tttgcacatt ttatgtgctt tgaagtacct 1620gcacttgttc cagctggttc tcctaatgct aataagagca aatccggagg aaatgacccg 1680gatgatgatc tgggtcttat ggatccagat ggcaacgatc tgaaacctga tagtacctct 1740gctgaactgg gtacattgaa tgaagctctt caagaattac tcgatggaat ccaggaagac 1800ttctatgcgg tcgtagattg ggcttataaa attgacccct tgcgttgcat ctcaatgcat 1860gggattacag agcgttacct ttctggacag aaagctgatg ctgcaggatt tgttcgcaaa 1920ctacttgatg acttggaatc aagaatatca gtacagttca gccggtttat tgatgaagca 1980tgccatcaaa ttgagcgtaa cgaaagaaat gtgcggcaaa ctggaattct agcctacatt 2040ccaagatttg ctgtccttgc atcacgtatg gaacaatata tccaagggca gtccagggat 2100ttaattgata aagcatacac aaagctagtt agcacaatgt tcgcaacttt ggagaaaatt 2160gcacagagtg atcctaaaac tgctgatatt gtgctgattg agaattatgc tgctttccag 2220aacagtcttt atgacttggc taatgttgtg ccaacgcttg caaagttcta ccatcaagcc 2280agtgaatcat atgaactagc ttgcactcgc catatcagct cactcattta tctgcaattt 2340gagaggttat ttcaattcaa ccggaaagtt gacgaattga cttacactat tgctgctgag 2400gagataccgt ttcagctggg attatcaaaa actgacctaa ggagggtact gaaatccagc 2460ttatctggga ttgataagtc gattagcgcc atgtacagga ggttacagaa gacacttacc 2520tccgatgagt tgtttccttc gctgtgggac aagtgcaaga aggagttttt ggacaaatac 2580gagagttttg ttcagatggt gacacggata tatgggaatg agccgatcat gtcagtcaac 2640gaaatgaaag acgtccttgc tggtttttag 2670231179DNAZea mays 23atgggcactc aaatagtaag ggaacaaacg agtggtagcc tcactgccat tgttgtggac 60gaaaatctat gccatgccag ggctgcaagc tgcatgcttg ccaacctcca gtgtaaagtg 120attgtgtatg cgagtcctgt tgatgcgctc aagtttctta aggatcacca aagagatact 180gattttgcgt tagtggaagt caacatgaaa gagatgcatg gttttcagtt ccttgacatg 240tccaggaagt tgcacaaaag ccttcaagtg atcatgatgt cagctgatac gacatggccc 300acgatgaaga gaagtgtcga gcttggtgca cgtttcttga ttaaaaagcc tcttgatgca 360aatacgatga ataacctgtg gcagcatctt gacctaaaat ttcaacggac cgacaagatc 420aaggccttat tcccaggtat tgaagggaaa acggggaatg catttgagga agggaccaac 480aagcagaagg gaactcacct gatgtggact ccattcctgc agaggaagtt tttgcaagct 540gttgaactac ttggggaaga cgcatctccg aagaagatac agttgctcat gaatgtgaac 600tccgttagtc gcaaacagat ctctgctcat cttcagaaac accgaaagaa ggtcgagaaa 660gaactacgta attcaaatgc aaacaactcc agccatggca ttggcggcgc ctcgaattca 720cggccttcga ggatttttga aataagccat ggcagattcc agtacaatcg tcccgacgtc 780caaccagaac atagatctga tgaatccgta tccgtggagc agacagaaac catcgaggaa 840acacaaagca acaggttgta tgaagcgatg cgaagagcct tgcagcttgg gagtgttttc 900gaggaaccac agttgcccaa tgatcctcct gctggtaaag atgcaagaga agttgaagaa 960gtcgaaatga cgatgaggga tggtaattat cgagatgctg gcacagatgc atttggtgat 1020aagaatgagg catctggaac tcacagttca gatggtaata acgcgaaggt caagagcaag 1080gatgattctg ctgacaagct ggtttcttgt cacgatgagc tgcgaccagt tgtgacgctt 1140gtgacctatt cagactcgga agatggtgaa acgctctag 117924768DNAZea mays 24atggggaggg gacgagttga gctcaagcgg atcgagaaca agatcaaccg ccaggtcacc 60ttctccaagc gccgcaacgg cctgctcaag aaggcctacg agctctccgt gctctgcgac 120gccgaggtcg cgctcatcat cttctccagc cgcggcaagc tctacgagtt cggcagcgcc 180ggcataacaa aaactttaga aaggtaccaa cattgctgct acaatgctca agattccaat 240ggcgcactct ctgaaactca gagctggtac caggaaatgt caaaactgag ggcaaaattc 300gaggccttgc agcgcactca gaggcacttg cttggggagg aacttggccc actgagtgtg 360aaggagttgc agcagctaga gaaacagctc gaatgtgctt tgtcacaggc aagacagaga 420aagacacaac ttatgatgga gcaagtggaa gagctccgca gaaaggagcg acacctggga 480gaaatgaaca ggcaactcaa acacaagctt gaagctgaag gttgtagcaa ctacagaacc 540ctgcagcatg cagcctggcc agctcccggc agcaccatgg tggagcatga cggcgccacc 600tatcatgtgc atccaacaac tgctcaatcg gttgcaatgg actgtgaacc cactctgcaa 660atcgggtacc ctcctcatca ccagtttctg ccttccgagg cagccaataa tatcccaagg 720agcccccctg gaggcgagaa caacttcatg ctgggatggg ttctttag 76825600DNAZea mays 25atggctgagg aggaggccaa gaaggtggag gtggaggtca ccaaggagcc cgaggcggca 60gcgaaggagg acgtagccga tgacaaggcc gtcatccccg cgaccgaccc gccgccgccg 120ccgccgccgg ccgacgactc caaggccctg gccatcgtcg agaaagttgc agatgaacct 180gctcccgaga agcctgcccc tgcgaagcaa gggggctcca atgacaggga tctcgctctt 240gcaagggtgg aaacagagaa gaggaactct ttgatcaaag cttgggaaga gaatgagaag 300acaaaagctg agaacaaggc tgctaagaaa gtatccgcta ttctttcatg ggaaaacaca 360aagaaagcaa acatagaagc tgaactgaag aagattgagg aacaactgga aaagaagaag 420gctgaatatg cagagaagat gaagaacaag gttgcaatga tacacaagga agccgaagag 480aagcgagcga tggtggaggc aaaacgcggt gaggaggtcc tgaaggccga ggagatggct 540gccaagtacc gggccaccgg ccacgctcct aagaagctca tcggttgctt cggagcctag 60026537DNAZea mays 26atggcggaag ctccggcgag ccctggcggc ggcggcggga gccacgagag cgggagcccc 60aggggaggcg gaggcggtgg cagcgtcagg gagcaggaca ggttcctgcc catcgccaac 120atcagtcgca tcatgaagaa ggccatcccg gctaacggga agatcgccaa ggacgctaag 180gagaccgtgc aggagtgcgt ctccgagttc atctccttca tcactagcga agcgagtgac 240aagtgccaga gggagaagcg gaagaccatc aatggcgacg atctgctgtg ggccatggcc 300acgctggggt ttgaagacta cattgaaccc ctcaaggtgt acctgcagaa gtacagagag 360atggagggtg atagcaagtt aactgcaaaa tctagcgatg gctcaattaa aaaggatgcc 420cttggtcatg tgggagcaag tagctcagct gcacaaggga tgggccaaca gggagcatac 480aaccaaggaa tgggttatat gcaaccccag taccataacg gggatatctc aaactga 53727642DNAZea mays 27atgtcgagcg gcagcggcag cgggaataag cgcgccgcgg cggagaggtc ggccggcgcc 60ggcgccggta gcggcgacga ggacgacgac ggtgccgcac gcaagaagct gcggctgtcc 120aaagaccagg ccgccgtgct cgaggagtgc ttcaagacgc accacacgct cactccgaag 180cagaaggtgg cgctggccag cagcctgggc ctccggccgc ggcaggtgga ggtgtggttc 240cagaaccggc gcgcccggac caagctgaag cagacggagg tggactgcga gtacctcaag 300cgctggtgcg agcagctcgc cgaggagaac cgccgcctgg gcaaggaggt cgccgagctc 360agggcgctca gcgccgcgcc ggcggccccg ctcaccaccc tcacgatgtg cctctcctgc 420cggcgcgtcg cctcctcgtc cccgtcgtcg tcgtcgtcgc ccaggcctag catccccggc 480gccgcagctg ccagtggcgg gagcatggcc tctccggcgg cggcggcgac gttgcccgcc 540cacaggcagt tcttctgcgg gttcagagac gccggggcgg cggccgcggc gtacgggaca 600gcctcggcgg ggctcgcgaa gcctgtcagg gctgccagat ag 642281272DNAArtificial Sequencecorn putative glutamine synthetase (with C299A mutation) 28atggcgcagg cggtggtgcc ggcgatgcag tgccgggtcg gagtgaaggc ggcggcgggg 60agggtgtgga gcgccggcag gactaggacc ggccgcggcg gcgcctcgcc ggggttcaag 120gtcatggccg tcagcacggg cagcaccggg gtggtgccgc gcctcgagca gctgctcaac 180atggacacca cgccctacac cgacaaggtc atcgccgagt acatctgggt cggaggatct 240ggaatcgaca tccgaagcaa atcaaggacg atttcgaaac ccgtggagga tccctcggaa 300ctaccaaaat ggaactacga tggatctagc acaggacaag ccccgggaga agacagtgaa 360gtcattctat acccccaggc tatcttcaag gacccattcc gaggtggcaa caacgttttg 420gttatctgtg acacctacac gccacagggg gaaccccttc caactaacaa acgccacagg 480gctgcgcaaa ttttcagtga cccaaaggtc gctgaacaag tgccatggtt tggcatagag 540caagagtaca ctttgctcca gaaagatgta aattggcctc ttggttggcc tgttggaggc 600ttccctggtc cccagggtcc atactactgt gccgtaggag ccgacaaatc atttggccgt 660gacatatcag atgctcacta caaggcatgc ctctacgctg gaatcaacat tagtggaaca 720aacggggagg tcatgcctgg tcagtgggag taccaagttg gacctagtgt tggtattgaa 780gcaggagatc acatatggat ttcgagatac attctcgaga gaatcacaga gcaagctggg 840gttgtcctta cccttgatcc aaaaccaatt cagggtgact ggaacggagc tggcgcacac 900acaaattaca gcacaaagac catgcgcgaa gacggcgggt ttgaagagat caagagagca 960atcctgaacc tttctctgcg ccatgatctg catattagtg catacggaga aggaaatgaa 1020agaagactga ctgggaaaca tgagactgcg agcatcggaa cgttctcatg gggtgtggca 1080aaccgcggct gctctatccg tgtggggcgg gataccgagg caaaagggaa aggttacctg 1140gaagaccgtc ggccggcatc aaacatggac ccgtacattg tgacggggct actggccgag 1200accacgatcc tctggcagcc atccctcgag gcggaggctc ttgccgccaa gaagctggcg 1260ctgaaggtgt ga 1272291785DNAZea mays 29atggtgaaga ggagcaagaa gagcaagagc aagcgagtca cgctgcggca gaagcacaag 60gtgcagcgca aggtgaagga gcaccaccgc aagaagcgca aggaggccaa gaaggcgggc 120aaggccggcc agcggaggaa ggtcgagaag gaccccggga tccccaacga gtggcccttc 180aaggagcagg agctcaaggc gctcgaggcc cgacgcgcgc aggcgctcca ggagctcgag 240ctcaagaagc aggcgcgcaa ggagagggct cagaagagga aggcaggatt gcttgaggac 300gaggacattg ctagtttggc atctgcagct tccgcacagg gcagtgagtt tgcagcaaag 360gagaatgctc ccttattagt ggcaaagatc aacgatcatt cagagaggtc tttttacaag 420gagcttgtta aagtaattga agcttccgat gtgattatgg aggttcttga tgcaagggat 480ccgctgggca cccgttgcat tgacatggaa aagatggtta ggaaggctga tccaagtaaa 540cggattgtac tacttctcaa caagatagat cttgttccca aggaggcggc agagaaatgg 600cttacatatc taagagaaga attgccaaca gttgctttta agtgcaatac ccaggagcag 660aggaccaagc tgggatggaa atcttcaaaa ttagataaaa caagcaacat cccacaaagc 720agtgattgtc ttggtgccga gaatctaatc aaattgctta agaattattc cagaagccat 780gagctcaaac tgacaattac ggtgggtatt gttggacttc ctaatgttgg caagagcagt 840ctgatcaaca gcctgaagag gtcccgagtg gtcaatgtcg gttctactcc agggattact 900agatcaatgc aagaagttca attagacaag aaggtaaagt tgttggattg tcctggtgtt 960gttatgctca aatcttccaa cagtggtgtg tctgtagccc ttcgaaactg caaaaaggtt 1020gagaaaatag aagatccagt cgctcctgtt aagcagatcc taagtatttg cccacatgag 1080aagttgctgt ctctatacaa ggttccaaac tttggttcag ttgatgattt tcttcagaaa 1140gtagcaactg tacgtgggaa attgaaaaag ggtggtgtag tggatgttga agctgcagcg 1200agaattgtgc ttcatgactg gaatgaaggt aaaataccat attttacact gccgcctaaa 1260agagatgctg gggaggactc agatgcagtg attatatccg aggatgggaa agaatttaat 1320atcgatgaga tttacaaagc tgaatcttca tatattggtg gtttgaagtc cattgaggag 1380ttccatcata ttgagattcc tcctaatgct ccactggcga tcgatgaaga gatgctagag 1440gatggtggca agaaaccaag cgaagccatc caggaaagcc gggacaggga ggagcaaatg 1500cccgacgtga aggactcagg aggaagcaag gcagccagcg ccagcacgca gaacgacaag 1560ttgtacaccg ccgagggcgt actcgaccct cgcaagagca aagcagagaa gaaacggcgc 1620aaggcgagca ggcccagcgc gctgaacgac atggacgcgg actacgattt caaggtggat 1680tatcggatgg aggacggtgg gagcgagggt gcacacgcgg atgatgaaga tggcggagat 1740ggatccgagg ataatgaacc aatgaccggt gtcgatgatg catga 178530305PRTAgrobacterium tumefaciens 30Met Ala Ser Pro Lys His Phe Leu Asp Leu Ser Ala Val Gly Pro Gln1 5 10 15Asp Leu Arg Thr Ile Leu Asp Asp Ala Arg Ala Arg Lys Val Ala Thr 20 25 30Lys Ala Gly Thr Ala Glu Lys Pro Leu Ala Gly Lys Met Leu Ala Met 35 40 45Ile Phe Glu Lys Pro Ser Thr Arg Thr Arg Val Ser Phe Asp Val Gly 50 55 60Met Arg Gln Leu Gly Gly Glu Thr Leu Phe Leu Ser Gly Thr Glu Met65 70 75 80Gln Leu Gly Arg Ala Glu Thr Ile Gly Asp Thr Ala Lys Val Leu Ser 85 90 95Arg Tyr Val Asp Ala Ile Met Ile Arg Thr Thr Asp His Ser Arg Leu 100 105 110Leu Glu Leu Ala Glu His Ala Thr Val Pro Val Ile Asn Gly Leu Thr 115 120 125Asp Asp Thr His Pro Cys Gln Ile Met Ala Asp Ile Met Thr Phe Glu 130 135 140Glu His Arg Gly Pro Val Lys Gly Lys Thr Ile Ala Trp Thr Gly Asp145 150 155 160Gly Asn Asn Val Leu His Ser Phe Val Glu Gly Ser Ala Arg Phe Gly 165 170 175Tyr Arg Met Thr Met Ala Val Pro Met Gly Ser Glu Pro His Asp Lys 180 185 190Phe Met Asn Trp Ala Arg Asn Asn Gly Gly Glu Ile Ala Leu Tyr His 195 200 205Asp Ala Asp Lys Ala Val Ala Gly Ala Asp Cys Val Val Thr Asp Thr 210 215 220Trp Val Ser Met Asn Gln Glu His Lys Ala Arg Gly His Asn Ile Phe225 230 235 240Gln Pro Tyr Gln Val Asn Glu Ala Leu Met Ala Lys Ala Gln Lys Asp 245 250 255Ala Leu Phe Met His Cys Leu Pro Ala His Arg Gly Glu Glu Val Thr 260 265 270Asp Ala Val Ile Asp Gly Pro Gln Ser Val Val Phe Asp Glu Ala Glu 275 280 285Asn Arg Leu His Ala Gln Lys Ser Val Ile Ala Trp Cys Met Gly Val 290 295 300Ile30531214PRTArabidopsis thaliana 31Met Val Arg Gly Lys Thr Gln Met Lys Arg Ile Glu Asn Ala Thr Ser1 5 10 15Arg Gln Val Thr Phe Ser Lys Arg Arg Asn Gly Leu Leu Lys Lys Ala 20 25 30Phe Glu Leu Ser Val Leu Cys Asp Ala Glu Val Ser Leu Ile Ile Phe 35 40 45Ser Pro Lys Gly Lys Leu Tyr Glu Phe Ala Ser Ser Asn Met Gln Asp 50 55 60Thr Ile Asp Arg Tyr Leu Arg His Thr Lys Asp Arg Val Ser Thr Lys65 70 75

80Pro Val Ser Glu Glu Asn Met Gln His Leu Lys Tyr Glu Ala Ala Asn 85 90 95Met Met Lys Lys Ile Glu Gln Leu Glu Ala Ser Lys Arg Lys Leu Leu 100 105 110Gly Glu Gly Ile Gly Thr Cys Ser Ile Glu Glu Leu Gln Gln Ile Glu 115 120 125Gln Gln Leu Glu Lys Ser Val Lys Cys Ile Arg Ala Arg Lys Thr Gln 130 135 140Val Phe Lys Glu Gln Ile Glu Gln Leu Lys Gln Lys Glu Lys Ala Leu145 150 155 160Ala Ala Glu Asn Glu Lys Leu Ser Glu Lys Trp Gly Ser His Glu Ser 165 170 175Glu Val Trp Ser Asn Lys Asn Gln Glu Ser Thr Gly Arg Gly Asp Glu 180 185 190Glu Ser Ser Pro Ser Ser Glu Val Glu Thr Gln Leu Phe Ile Gly Leu 195 200 205Pro Cys Ser Ser Arg Lys 210321006PRTArabidopsis thaliana 32Met Gly Glu Gln Gln Ile Ser Val Thr Val Pro Arg Asp Arg Thr Asp1 5 10 15Glu Gln Ala Ile Leu Ala Leu Lys Lys Gly Ala Gln Leu Leu Lys Cys 20 25 30Arg Arg Arg Gly Asn Pro Lys Phe Cys Pro Phe Lys Leu Ser Met Asp 35 40 45Glu Lys Tyr Leu Ile Trp Tyr Ser Gly Glu Glu Glu Arg Gln Leu Arg 50 55 60Leu Ser Ser Val Ile Thr Ile Val Arg Gly Gln Ile Thr Pro Asn Phe65 70 75 80Gln Lys Gln Ala Gln Ser Asp Arg Lys Glu Gln Ser Phe Ser Leu Ile 85 90 95Tyr Ala Asn Gly Glu His Thr Leu Asp Leu Ile Cys Lys Asp Lys Ala 100 105 110Gln Ala Asp Ser Trp Phe Lys Gly Leu Arg Ala Val Ile Thr Lys His 115 120 125His Asn Ile Arg Asn Ser Val Asn His Arg Ser Ser Arg Gly Ala Gln 130 135 140Ser Cys Ile Asn Ser Pro Ala Gly Phe Met Arg Arg Lys Gln Asn Leu145 150 155 160Gly Leu Leu Glu Glu Thr Pro Asp Val Thr Gln Ile Arg Ser Leu Cys 165 170 175Gly Ser Pro Ser Thr Leu Leu Glu Glu Arg Cys Leu Ser Asn Gly Leu 180 185 190Ser Cys Ser Ser Asp Ser Phe Ala Glu Ser Asp Ala Leu Gly Pro Val 195 200 205Ser Ser Tyr Tyr Glu Thr Asp Tyr Asp Phe Arg Asn Ser Asp Cys Asp 210 215 220Arg Ser Thr Gly Ser Glu Leu Cys Arg Phe Ser Ser Gln Arg Phe Ala225 230 235 240Ala Ser Pro Pro Leu Ser Ile Ile Thr Gln Pro Val Thr Arg Ser Asn 245 250 255Val Leu Lys Asp Ile Met Ile Trp Gly Ala Ile Thr Gly Leu Ile Asp 260 265 270Gly Ser Lys Asn Gln Asn Asp Ala Leu Ser Pro Lys Leu Leu Glu Ser 275 280 285Ala Thr Met Phe Asp Val Gln Ser Ile Ser Leu Gly Ala Lys His Ala 290 295 300Ala Leu Val Thr Arg Gln Gly Glu Val Phe Cys Trp Gly Asn Gly Asn305 310 315 320Ser Gly Lys Leu Gly Leu Lys Val Asn Ile Asp Ile Asp His Pro Lys 325 330 335Arg Val Glu Ser Leu Glu Asp Val Ala Val Arg Ser Val Ala Cys Ser 340 345 350Asp His Gln Thr Cys Ala Val Thr Glu Ser Gly Glu Leu Tyr Leu Trp 355 360 365Gly Ile Asp Gly Gly Thr Ile Glu Gln Ser Gly Ser Gln Phe Leu Thr 370 375 380Arg Lys Ile Ser Asp Val Leu Gly Gly Ser Leu Thr Val Leu Ser Val385 390 395 400Ala Cys Gly Ala Trp His Thr Ala Ile Val Thr Ser Ser Gly Gln Leu 405 410 415Phe Thr Tyr Gly Ser Gly Thr Phe Gly Val Leu Gly His Gly Ser Leu 420 425 430Glu Ser Val Thr Lys Pro Lys Glu Val Glu Ser Leu Arg Arg Met Lys 435 440 445Val Ile Ser Val Ser Cys Gly Pro Trp His Thr Ala Ala Ile Val Glu 450 455 460Thr Ala Asn Asp Arg Lys Phe Tyr Asn Ala Lys Ser Cys Gly Lys Leu465 470 475 480Phe Thr Trp Gly Asp Gly Asp Lys Gly Arg Leu Gly His Ala Asp Ser 485 490 495Lys Arg Lys Leu Val Pro Thr Cys Val Thr Glu Leu Ile Asp His Asp 500 505 510Phe Ile Lys Val Ser Cys Gly Trp Thr Leu Thr Val Ala Leu Ser Ile 515 520 525Ser Gly Thr Val Tyr Thr Met Gly Ser Ser Ile His Gly Gln Leu Gly 530 535 540Cys Pro Arg Ala Lys Asp Lys Ser Val Asn Val Val Leu Gly Asn Leu545 550 555 560Thr Arg Gln Phe Val Lys Asp Ile Ala Ser Gly Ser His His Val Ala 565 570 575Val Leu Thr Ser Phe Gly Asn Val Tyr Thr Trp Gly Lys Gly Met Asn 580 585 590Gly Gln Leu Gly Leu Gly Asp Val Arg Asp Arg Asn Ser Pro Val Leu 595 600 605Val Glu Pro Leu Gly Asp Arg Leu Val Glu Ser Ile Ala Cys Gly Leu 610 615 620Asn Leu Thr Ala Ala Ile Cys Leu His Lys Glu Ile Ser Leu Asn Asp625 630 635 640Gln Thr Ala Cys Ser Ser Cys Lys Ser Ala Phe Gly Phe Thr Arg Arg 645 650 655Lys His Asn Cys Tyr Asn Cys Gly Leu Leu Phe Cys Asn Ala Cys Ser 660 665 670Ser Lys Lys Ala Val Asn Ala Ser Leu Ala Pro Asn Lys Ser Lys Leu 675 680 685Ser Arg Val Cys Asp Ser Cys Phe Asp His Leu Trp Ser Ile Thr Glu 690 695 700Phe Ser Arg Asn Val Lys Met Asp Asn His Thr Pro Arg Met Gln Met705 710 715 720Val Thr Arg Arg Val Ser Glu Asp Leu Thr Glu Lys Gln Ser Glu Asn 725 730 735Glu Met Gln Asn Leu Pro Gln Ala Asn Arg Ser Ser Asp Gly Gln Pro 740 745 750Arg Trp Gly Gln Val Ser Gly Pro Ser Leu Phe Arg Phe Asp Lys Ile 755 760 765Ser Thr Ser Ser Ser Leu Asn Leu Ser Val Ser Ala Arg Arg Thr Ser 770 775 780Ser Thr Lys Ile Ser Thr Ser Ser Glu Ser Asn Lys Ile Leu Thr Glu785 790 795 800Glu Ile Glu Arg Leu Lys Ala Val Ile Lys Asn Leu Gln Arg Gln Cys 805 810 815Glu Leu Gly Asn Glu Lys Met Glu Glu Cys Gln Gln Glu Leu Asp Lys 820 825 830Thr Trp Glu Val Ala Lys Glu Glu Ala Glu Lys Ser Lys Ala Ala Lys 835 840 845Glu Ile Ile Lys Ala Leu Ala Ser Lys Leu Gln Ala Asn Lys Glu Lys 850 855 860Pro Ser Asn Pro Leu Lys Thr Gly Ile Ala Cys Asn Pro Ser Gln Val865 870 875 880Ser Pro Ile Phe Asp Asp Ser Met Ser Ile Pro Tyr Leu Thr Pro Ile 885 890 895Thr Thr Ala Arg Ser Gln His Glu Thr Lys Gln His Val Glu Lys Cys 900 905 910Val Thr Lys Ser Ser Asn Arg Asp Ser Asn Ile Lys Leu Leu Val Asp 915 920 925Ala Ser Pro Ala Ile Thr Arg Thr Gly Tyr Leu Gln Asn Glu Thr Gln 930 935 940Asp Ser Ser Ala Glu Gln Val Glu Gln Tyr Glu Pro Gly Val Tyr Ile945 950 955 960Thr Phe Thr Ala Leu Pro Cys Gly Gln Lys Thr Leu Lys Arg Val Arg 965 970 975Phe Ser Arg Lys Arg Phe Ser Glu Lys Glu Ala Gln Arg Trp Trp Glu 980 985 990Glu Lys Gln Val Leu Val Tyr Asn Lys Tyr Asp Ala Glu Ile 995 1000 100533729PRTArabidopsis thaliana 33Met Arg Ile Phe Ser Thr Phe Val Phe His Arg Arg Gln Gln Ile Phe1 5 10 15Asn Leu Arg Gln Phe Gln Thr Thr Thr Ile Leu Arg Asn Pro Ile Ser 20 25 30Ile Ala Pro Ile Gln Ile Pro Met Asp Ala Thr Glu Gln Ser Leu Arg 35 40 45Gln Ser Leu Ser Glu Lys Ser Ser Ser Val Glu Ala Gln Gly Asn Ala 50 55 60Val Arg Ala Leu Lys Ala Ser Arg Ala Ala Lys Pro Glu Ile Asp Ala65 70 75 80Ala Ile Glu Gln Leu Asn Lys Leu Lys Leu Glu Lys Ser Thr Val Glu 85 90 95Lys Glu Leu Gln Ser Ile Ile Ser Ser Ser Gly Asn Gly Ser Leu Asn 100 105 110Arg Glu Ala Phe Arg Lys Ala Val Val Asn Thr Leu Glu Arg Arg Leu 115 120 125Phe Tyr Ile Pro Ser Phe Lys Ile Tyr Ser Gly Val Ala Gly Leu Phe 130 135 140Asp Tyr Gly Pro Pro Gly Cys Ala Ile Lys Ser Asn Val Leu Ser Phe145 150 155 160Trp Arg Gln His Phe Ile Leu Glu Glu Asn Met Leu Glu Val Asp Cys 165 170 175Pro Cys Val Thr Pro Glu Val Val Leu Lys Ala Ser Gly His Val Asp 180 185 190Lys Phe Thr Asp Leu Met Val Lys Asp Glu Lys Thr Gly Thr Cys Tyr 195 200 205Arg Ala Asp His Leu Leu Lys Asp Tyr Cys Thr Glu Lys Leu Glu Lys 210 215 220Asp Leu Thr Ile Ser Ala Glu Lys Ala Ala Glu Leu Lys Asp Val Leu225 230 235 240Ala Val Met Glu Asp Phe Ser Pro Glu Gln Leu Gly Ala Lys Ile Arg 245 250 255Glu Tyr Gly Ile Thr Ala Pro Asp Thr Lys Asn Pro Leu Ser Asp Pro 260 265 270Tyr Pro Phe Asn Leu Met Phe Gln Thr Ser Ile Gly Pro Ser Gly Leu 275 280 285Ile Pro Gly Tyr Met Arg Pro Glu Thr Ala Gln Gly Ile Phe Val Asn 290 295 300Phe Lys Asp Leu Tyr Tyr Tyr Asn Gly Lys Lys Leu Pro Phe Ala Ala305 310 315 320Ala Gln Ile Gly Gln Ala Phe Arg Asn Glu Ile Ser Pro Arg Gln Gly 325 330 335Leu Leu Arg Val Arg Glu Phe Thr Leu Ala Glu Ile Glu His Phe Val 340 345 350Asp Pro Glu Asn Lys Ser His Pro Lys Phe Ser Asp Val Ala Lys Leu 355 360 365Glu Phe Leu Met Phe Pro Arg Glu Glu Gln Met Ser Gly Gln Ser Ala 370 375 380Lys Lys Leu Cys Leu Gly Glu Ala Val Ala Lys Gly Thr Val Asn Asn385 390 395 400Glu Thr Leu Gly Tyr Phe Ile Gly Arg Val Tyr Leu Phe Leu Thr Arg 405 410 415Leu Gly Ile Asp Lys Glu Arg Leu Arg Phe Arg Gln His Leu Ala Asn 420 425 430Glu Met Ala His Tyr Ala Ala Asp Cys Trp Asp Ala Glu Ile Glu Ser 435 440 445Ser Tyr Gly Trp Ile Glu Cys Val Gly Ile Ala Asp Arg Ser Ala Tyr 450 455 460Asp Leu Arg Ala His Ser Asp Lys Ser Gly Thr Pro Leu Val Ala Glu465 470 475 480Glu Lys Phe Ala Glu Pro Lys Glu Val Glu Lys Leu Val Ile Thr Pro 485 490 495Val Lys Lys Glu Leu Gly Leu Ala Phe Lys Gly Asn Gln Lys Asn Val 500 505 510Val Glu Ser Leu Glu Ala Met Asn Glu Glu Glu Ala Met Glu Met Lys 515 520 525Ala Thr Leu Glu Ser Lys Gly Glu Val Glu Phe Tyr Val Cys Thr Leu 530 535 540Lys Lys Ser Val Asn Ile Lys Lys Asn Met Val Ser Ile Ser Lys Glu545 550 555 560Lys Lys Lys Glu His Gln Arg Val Phe Thr Pro Ser Val Ile Glu Pro 565 570 575Ser Phe Gly Ile Gly Arg Ile Ile Tyr Cys Leu Tyr Glu His Cys Phe 580 585 590Ser Thr Arg Pro Ser Lys Ala Gly Asp Glu Gln Leu Asn Leu Phe Arg 595 600 605Phe Pro Pro Leu Val Ala Pro Ile Lys Cys Thr Val Phe Pro Leu Val 610 615 620Gln Asn Gln Gln Phe Glu Glu Val Ala Lys Val Ile Ser Lys Glu Leu625 630 635 640Ala Ser Val Gly Ile Ser His Lys Ile Asp Ile Thr Gly Thr Ser Ile 645 650 655Gly Lys Arg Tyr Ala Arg Thr Asp Glu Leu Gly Val Pro Phe Ala Ile 660 665 670Thr Val Asp Ser Asp Thr Ser Val Thr Ile Arg Glu Arg Asp Ser Lys 675 680 685Asp Gln Val Arg Val Thr Leu Lys Glu Ala Ala Ser Val Val Ser Ser 690 695 700Val Ser Glu Gly Lys Met Thr Trp Gln Asp Val Trp Ala Thr Phe Pro705 710 715 720His His Ser Ser Ala Ala Ala Asp Glu 72534588PRTArabidopsis thaliana 34Met Ser Tyr Asp Glu His Thr Ser Ser Ser Phe Thr Tyr Ile Ser Gln1 5 10 15Met Gly Val Trp Trp Ile Val Leu Val Val Ala Val Leu Thr His Thr 20 25 30Ala Ser Ala Ala Val Arg Glu Tyr His Trp Glu Val Glu Tyr Lys Tyr 35 40 45Trp Ser Pro Asp Cys Lys Glu Gly Ala Val Met Thr Val Asn Gly Glu 50 55 60Phe Pro Gly Pro Thr Ile Lys Ala Phe Ala Gly Asp Thr Ile Val Val65 70 75 80Asn Leu Thr Asn Lys Leu Thr Thr Glu Gly Leu Val Ile His Trp His 85 90 95Gly Ile Arg Gln Phe Gly Ser Pro Trp Ala Asp Gly Ala Ala Gly Val 100 105 110Thr Gln Cys Ala Ile Asn Pro Gly Glu Thr Phe Thr Tyr Asn Phe Thr 115 120 125Val Glu Lys Pro Gly Thr His Phe Tyr His Gly His Tyr Gly Met Gln 130 135 140Arg Ser Ala Gly Leu Tyr Gly Ser Leu Ile Val Asp Val Ala Lys Gly145 150 155 160Lys Ser Glu Arg Leu Arg Tyr Asp Gly Glu Phe Asn Leu Leu Leu Ser 165 170 175Asp Trp Trp His Glu Ala Ile Pro Ser Gln Glu Leu Gly Leu Ser Ser 180 185 190Lys Pro Met Arg Trp Ile Gly Glu Ala Gln Ser Ile Leu Ile Asn Gly 195 200 205Arg Gly Gln Phe Asn Cys Ser Leu Ala Ala Gln Phe Ser Asn Asn Thr 210 215 220Ser Leu Pro Met Cys Thr Phe Lys Glu Gly Asp Gln Cys Ala Pro Gln225 230 235 240Ile Leu His Val Glu Pro Asn Lys Thr Tyr Arg Ile Arg Leu Ser Ser 245 250 255Thr Thr Ala Leu Ala Ser Leu Asn Leu Ala Val Gln Gly His Lys Leu 260 265 270Val Val Val Glu Ala Asp Gly Asn Tyr Ile Thr Pro Phe Thr Thr Asp 275 280 285Asp Ile Asp Ile Tyr Ser Gly Glu Ser Tyr Ser Val Leu Leu Thr Thr 290 295 300Asp Gln Asp Pro Ser Gln Asn Tyr Tyr Ile Ser Val Gly Val Arg Gly305 310 315 320Arg Lys Pro Asn Thr Thr Gln Ala Leu Thr Ile Leu Asn Tyr Val Thr 325 330 335Ala Pro Ala Ser Lys Leu Pro Ser Ser Pro Pro Pro Val Thr Pro Arg 340 345 350Trp Asp Asp Phe Glu Arg Ser Lys Asn Phe Ser Lys Lys Ile Phe Ser 355 360 365Ala Met Gly Ser Pro Ser Pro Pro Lys Lys Tyr Arg Lys Arg Leu Ile 370 375 380Leu Leu Asn Thr Gln Asn Leu Ile Asp Gly Tyr Thr Lys Trp Ala Ile385 390 395 400Asn Asn Val Ser Leu Val Thr Pro Ala Thr Pro Tyr Leu Gly Ser Val 405 410 415Lys Tyr Asn Leu Lys Leu Gly Phe Asn Arg Lys Ser Pro Pro Arg Ser 420 425 430Tyr Arg Met Asp Tyr Asp Ile Met Asn Pro Pro Pro Phe Pro Asn Thr 435 440 445Thr Thr Gly Asn Gly Ile Tyr Val Phe Pro Phe Asn Val Thr Val Asp 450 455 460Val Ile Ile Gln Asn Ala Asn Val Leu Lys Gly Ile Val Ser Glu Ile465 470 475 480His Pro Trp His Leu His Gly His Asp Phe Trp Val Leu Gly Tyr Gly 485 490 495Asp Gly Lys Phe Lys Pro Gly Ile Asp Glu Lys Thr Tyr Asn Leu Lys 500 505 510Asn Pro Pro Leu Arg Asn Thr Ala Ile Leu Tyr Pro Tyr Gly Trp Thr 515 520 525Ala Ile Arg Phe Val Thr Asp Asn Pro Gly Val Trp Phe Phe His Cys 530 535 540His Ile Glu Pro His Leu His Met Gly Met Gly Val Val Phe Ala Glu545 550 555 560Gly Leu Asn Arg Ile Gly Lys Val Pro Asp Glu Ala Leu Gly Cys Gly 565 570 575Leu Thr Lys Gln Phe Leu Met Asn Arg Asn Arg Asn 580 58535493PRTArabidopsis thaliana 35Met Val Leu Ser Lys Thr Val Ser

Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Asn Ser Leu Pro Arg Phe Glu Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Gln Gly Leu Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Asn Leu Arg Glu Asp Tyr Tyr Val Met Asp Pro 180 185 190Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Thr Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Gln Gly Leu Glu Lys Thr Gly Arg Phe Lys Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asn Glu Phe Glu Val Ala His Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val His Ala Lys Met Ala Asn Gly Lys Val Asn Gly Val Lys 450 455 460Lys Thr Pro Glu Glu Thr Gln Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Leu Leu Glu Thr Lys Lys Thr Asn Lys Asn Thr Ile Cys 485 49036282PRTArabidopsis thaliana 36Met Gly Thr Arg Ala Gln Gln Ile Pro Leu Leu Glu Gly Glu Thr Asp1 5 10 15Asn Tyr Asp Gly Val Thr Val Thr Met Val Glu Pro Met Asp Ser Glu 20 25 30Val Phe Thr Glu Ser Leu Arg Ala Ser Leu Ser His Trp Arg Glu Glu 35 40 45Gly Lys Lys Gly Ile Trp Ile Lys Leu Pro Leu Gly Leu Ala Asn Leu 50 55 60Val Glu Ala Ala Val Ser Glu Gly Phe Arg Tyr His His Ala Glu Pro65 70 75 80Glu Tyr Leu Met Leu Val Ser Trp Ile Ser Glu Thr Pro Asp Thr Ile 85 90 95Pro Ala Asn Ala Ser His Val Val Gly Ala Gly Ala Leu Val Ile Asn 100 105 110Lys Asn Thr Lys Glu Val Leu Val Val Gln Glu Arg Ser Gly Phe Phe 115 120 125Lys Asp Lys Asn Val Trp Lys Leu Pro Thr Gly Val Ile Asn Glu Gly 130 135 140Glu Asp Ile Trp Thr Gly Val Ala Arg Glu Val Glu Glu Glu Thr Gly145 150 155 160Ile Ile Ala Asp Phe Val Glu Val Leu Ala Phe Arg Gln Ser His Lys 165 170 175Ala Ile Leu Lys Lys Lys Thr Asp Met Phe Phe Leu Cys Val Leu Ser 180 185 190Pro Arg Ser Tyr Asp Ile Thr Glu Gln Lys Ser Glu Ile Leu Gln Ala 195 200 205Lys Trp Met Pro Ile Gln Glu Tyr Val Asp Gln Pro Trp Asn Lys Lys 210 215 220Asn Glu Met Phe Lys Phe Met Ala Asn Ile Cys Gln Lys Lys Cys Glu225 230 235 240Glu Glu Tyr Leu Gly Phe Ala Ile Val Pro Thr Thr Thr Ser Ser Gly 245 250 255Lys Glu Ser Phe Ile Tyr Cys Asn Ala Asp His Ala Lys Arg Leu Lys 260 265 270Val Ser Arg Asp Gln Ala Ser Ala Ser Leu 275 28037460PRTArabidopsis thaliana 37Met Thr Val Val Gly Asp Val Ala Pro Ile Pro Arg Arg Asn Ser Ser1 5 10 15Thr Cys Ser Asn Asp Ile Ala Ala Pro Leu Leu Pro Glu Cys His Gly 20 25 30Asp Glu Val Ala His Asp Glu Phe Asn Gly Ala Ser Phe Ser Gly Ala 35 40 45Val Phe Asn Leu Ala Thr Thr Ile Ile Gly Ala Gly Ile Met Ala Leu 50 55 60Pro Ala Thr Met Lys Ile Leu Gly Leu Gly Leu Gly Ile Thr Met Ile65 70 75 80Val Val Met Ala Phe Leu Thr Asp Ala Ser Ile Glu Phe Leu Leu Arg 85 90 95Phe Ser Lys Ala Gly Lys Asn Arg Ser Tyr Gly Gly Leu Met Gly Gly 100 105 110Ser Phe Gly Asn Pro Gly Arg Ile Leu Leu Gln Val Ala Val Leu Val 115 120 125Asn Asn Ile Gly Val Leu Ile Val Tyr Met Ile Ile Ile Gly Asp Val 130 135 140Leu Ala Gly Lys Thr Glu Asp Gly Ile His His Phe Gly Val Leu Glu145 150 155 160Gly Trp Phe Gly His His Trp Trp Asn Gly Arg Ala Ala Ile Leu Leu 165 170 175Ile Thr Thr Leu Gly Val Phe Ala Pro Leu Ala Cys Phe Lys Arg Ile 180 185 190Asp Ser Leu Lys Phe Thr Ser Ala Leu Ser Val Ala Leu Ala Val Val 195 200 205Phe Leu Ile Ile Thr Ala Gly Ile Ser Ile Met Lys Leu Ile Ser Gly 210 215 220Gly Val Ala Met Pro Arg Leu Leu Pro Asp Val Thr Asp Leu Thr Ser225 230 235 240Phe Trp Asn Leu Phe Thr Val Val Pro Val Leu Val Thr Ala Phe Ile 245 250 255Cys His Tyr Asn Val His Ser Ile Gln Asn Glu Leu Glu Asp Pro Ser 260 265 270Gln Ile Arg Pro Val Val Arg Ser Ala Leu Met Leu Cys Ser Ser Val 275 280 285Tyr Ile Met Thr Ser Ile Phe Gly Phe Leu Leu Phe Gly Asp Asp Thr 290 295 300Leu Asp Asp Val Leu Ala Asn Phe Asp Thr Asp Leu Gly Ile Pro Phe305 310 315 320Gly Ser Ile Leu Asn Asp Ala Val Arg Val Ser Tyr Ala Leu His Leu 325 330 335Met Leu Val Phe Pro Ile Val Phe Tyr Pro Leu Arg Ile Asn Ile Asp 340 345 350Gly Leu Leu Phe Pro Ser Ala Arg Ser Leu Ser Thr Ser Asn Val Arg 355 360 365Phe Gly Cys Leu Thr Ala Gly Leu Ile Ser Val Ile Phe Leu Gly Ala 370 375 380Asn Phe Ile Pro Ser Ile Trp Asp Ala Phe Gln Phe Thr Gly Ala Thr385 390 395 400Ala Ala Val Cys Leu Gly Phe Ile Phe Pro Ala Ser Ile Ile Leu Lys 405 410 415Asp Arg His Asp Lys Ala Thr Asn Arg Asp Thr Thr Leu Ala Ile Phe 420 425 430Met Ile Val Leu Ala Val Leu Ser Asn Ala Ile Ala Ile Tyr Ser Asp 435 440 445Ala Tyr Ala Leu Phe Lys Lys Asn Ala Pro Arg Glu 450 455 46038133PRTArtificial SequenceArabidopsis thaliana putative AP2/EREBP transcription factor, with A33V mutationMUTAGEN(33)..(33)A33V mutation 38Met Asp Gln Gly Gly Arg Ser Ser Gly Ser Gly Gly Gly Gly Ala Glu1 5 10 15Gln Gly Lys Tyr Arg Gly Val Arg Arg Arg Pro Trp Gly Lys Tyr Ala 20 25 30Val Glu Ile Arg Asp Ser Arg Lys His Gly Glu Arg Val Trp Leu Gly 35 40 45Thr Phe Asp Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Arg Ala Ala 50 55 60Tyr Ser Met Arg Gly Lys Ala Ala Ile Leu Asn Phe Pro His Glu Tyr65 70 75 80Asn Met Gly Thr Gly Ser Ser Ser Thr Ala Ala Asn Ser Ser Ser Ser 85 90 95Ser Gln Gln Val Phe Glu Phe Glu Tyr Leu Asp Asp Ser Val Leu Asp 100 105 110Glu Leu Leu Glu Tyr Gly Glu Asn Tyr Asn Lys Thr His Asn Ile Asn 115 120 125Met Gly Lys Arg Gln 13039357PRTArabidopsis thaliana 39Met Val Ile Tyr His Arg Lys Val Val Phe Thr Tyr Val Arg Ala Lys1 5 10 15Arg Phe Tyr His Phe Leu Asn Ile Glu Met Val Thr Asp Phe Lys Ser 20 25 30Leu Leu Pro Val Ile Asp Ile Ser Pro Leu Leu Ala Lys Cys Asp Asp 35 40 45Phe Asp Met Ala Glu Asp Ala Gly Val Val Glu Val Val Gly Lys Leu 50 55 60Asp Arg Ala Cys Arg Asp Val Gly Phe Phe Tyr Val Ile Gly His Gly65 70 75 80Ile Ser Asp Asp Leu Ile Asn Lys Val Lys Glu Met Thr His Gln Phe 85 90 95Phe Glu Leu Pro Tyr Glu Glu Lys Leu Lys Ile Lys Ile Thr Pro Thr 100 105 110Ala Gly Tyr Arg Gly Tyr Gln Arg Ile Gly Val Asn Phe Thr Ser Gly 115 120 125Lys Gln Asp Met His Glu Ala Ile Asp Cys Tyr Arg Glu Phe Lys Gln 130 135 140Gly Lys His Gly Asp Ile Gly Lys Val Leu Glu Gly Pro Asn Gln Trp145 150 155 160Pro Gly Asn Pro Gln Glu Tyr Lys Asp Leu Met Glu Lys Tyr Ile Lys 165 170 175Leu Cys Thr Asp Leu Ser Arg Asn Ile Leu Arg Gly Ile Ser Leu Ala 180 185 190Leu Gly Gly Ser Pro Tyr Glu Phe Glu Gly Lys Met Leu Arg Asp Pro 195 200 205Phe Trp Val Met Arg Ile Ile Gly Tyr Pro Gly Val Asn Gln Glu Asn 210 215 220Val Ile Gly Cys Gly Ala His Thr Asp Tyr Gly Leu Leu Thr Leu Ile225 230 235 240Asn Gln Asp Asp Asp Lys Thr Ala Leu Gln Val Lys Asn Val Asp Gly 245 250 255Asp Trp Ile Pro Ala Ile Pro Ile Pro Gly Ser Phe Ile Cys Asn Ile 260 265 270Gly Asp Met Leu Thr Ile Leu Ser Asn Gly Val Tyr Gln Ser Thr Leu 275 280 285His Lys Val Ile Asn Asn Ser Pro Lys Tyr Arg Val Cys Val Ala Phe 290 295 300Phe Tyr Glu Thr Asn Phe Glu Ala Glu Val Glu Pro Leu Asp Ile Phe305 310 315 320Lys Glu Lys His Pro Arg Lys Glu Thr Ser Gln Val Ala Lys Arg Val 325 330 335Val Tyr Gly Gln His Leu Ile Asn Lys Val Leu Thr Thr Phe Ala Asn 340 345 350Leu Val Glu Asn Ser 35540371PRTArabidopsis thaliana 40Met Pro Val Leu Phe Ser Ser Arg Ser Leu Ile Leu Ser Ile Ile Val1 5 10 15Pro Leu Leu Ile Ser Ile Ala Leu Tyr Lys Leu Asp Thr Phe Asp Pro 20 25 30Ala Ile Val Pro Ser Asp Ala Phe Thr Ser Ser Ala Thr Ser Leu Pro 35 40 45Pro Leu Ile Asn Asp Glu Phe Leu Thr Gly Ala Glu Phe Ile Gly Val 50 55 60Gly Leu Leu Asn Ile Pro Glu Asp Ile Ala Tyr His Lys Glu Ser Asn65 70 75 80Leu Ile Tyr Thr Gly Cys Val Asp Gly Trp Val Lys Arg Val Lys Val 85 90 95Ala Asp Ser Val Asn Asp Ser Val Val Glu Asp Trp Val Asn Thr Gly 100 105 110Gly Arg Pro Leu Gly Ile Ala Phe Gly Ile His Gly Glu Val Ile Val 115 120 125Ala Asp Val His Lys Gly Leu Leu Asn Ile Ser Gly Asp Gly Lys Lys 130 135 140Thr Glu Leu Leu Thr Asp Glu Ala Asp Gly Val Lys Phe Lys Leu Thr145 150 155 160Asp Ala Val Thr Val Ala Asp Asn Gly Val Leu Tyr Phe Thr Asp Ala 165 170 175Ser Tyr Lys Tyr Thr Leu Asn Gln Leu Ser Leu Asp Met Leu Glu Gly 180 185 190Lys Pro Phe Gly Arg Leu Leu Ser Phe Asp Pro Thr Thr Arg Val Thr 195 200 205Lys Val Leu Leu Lys Asp Leu Tyr Phe Ala Asn Gly Ile Thr Ile Ser 210 215 220Pro Asp Gln Thr His Leu Ile Phe Cys Glu Thr Pro Met Lys Arg Cys225 230 235 240Ser Lys Tyr Tyr Ile Ser Glu Glu Arg Val Glu Val Phe Thr Gln Ser 245 250 255Leu Pro Gly Tyr Pro Asp Asn Ile Arg Tyr Asp Gly Asp Gly His Tyr 260 265 270Trp Ile Ala Leu Pro Ser Gly Val Thr Thr Leu Trp Asn Ile Ser Leu 275 280 285Lys Tyr Pro Phe Leu Arg Lys Leu Thr Ala Met Val Ala Lys Tyr Gly 290 295 300Val Asp Leu Met Phe Met Glu Asn Ala Gly Val Leu Gln Val Asp Leu305 310 315 320Asp Gly Asn Pro Ile Ala Tyr Tyr His Asp Pro Lys Leu Ser His Ile 325 330 335Ala Thr Cys Asp Lys Ile Gly Lys Tyr Leu Tyr Cys Gly Ser Leu Ser 340 345 350Gln Ser His Ile Leu Arg Leu Asp Leu Leu Lys Tyr Pro Ala Gln Asn 355 360 365Lys Lys Leu 37041468PRTArabidopsis thaliana 41Met Gly Arg Val Arg Lys Ser Asp Phe Gly Ser Ile Val Leu Val Leu1 5 10 15Cys Cys Val Leu Asn Ser Leu Leu Cys Asn Gly Gly Ile Thr Ser Arg 20 25 30Tyr Val Arg Lys Leu Glu Ala Thr Val Asp Met Pro Leu Asp Ser Asp 35 40 45Val Phe Arg Val Pro Cys Gly Tyr Asn Ala Pro Gln Gln Val His Ile 50 55 60Thr Gln Gly Asp Val Glu Gly Lys Ala Val Ile Val Ser Trp Val Thr65 70 75 80Gln Glu Ala Lys Gly Ser Asn Lys Val Ile Tyr Trp Lys Glu Asn Ser 85 90 95Thr Lys Lys His Lys Ala His Gly Lys Thr Asn Thr Tyr Lys Phe Tyr 100 105 110Asn Tyr Thr Ser Gly Phe Ile His His Cys Pro Ile Arg Asn Leu Glu 115 120 125Tyr Asp Thr Lys Tyr Tyr Tyr Val Leu Gly Val Gly Gln Thr Glu Arg 130 135 140Lys Phe Trp Phe Phe Thr Pro Pro Glu Ile Gly Pro Asp Val Pro Tyr145 150 155 160Thr Phe Gly Leu Ile Gly Asp Leu Gly Gln Ser Tyr Asp Ser Asn Ile 165 170 175Thr Leu Thr His Tyr Glu Asn Asn Pro Thr Lys Gly Gln Ala Val Leu 180 185 190Phe Val Gly Asp Ile Ser Tyr Ala Asp Thr Tyr Pro Asp His Asp Asn 195 200 205Arg Arg Trp Asp Ser Trp Gly Arg Phe Ala Glu Arg Ser Thr Ala Tyr 210 215 220Gln Pro Trp Ile Trp Thr Thr Gly Asn His Glu Leu Asp Phe Ala Pro225 230 235 240Glu Ile Gly Glu Asn Arg Pro Phe Lys Pro Phe Thr His Arg Tyr Arg 245 250 255Thr Pro Tyr Arg Ser Ser Gly Ser Thr Glu Pro Phe Trp Tyr Ser Ile 260 265 270Lys Arg Gly Pro Ala Tyr Ile Ile Val Leu Ala Ser Tyr Ser Ala Tyr 275 280 285Gly Lys Tyr Thr Pro Gln Tyr Gln Trp Leu Glu Glu Glu Phe Pro Lys 290 295 300Val Asn Arg Thr Glu Thr Pro Trp Leu Ile Val Leu Met His Ser Pro305 310 315 320Trp Tyr Asn Ser Tyr Asp Tyr His Tyr Met Glu Gly Glu Thr Met Arg 325 330 335Val Met Tyr Glu Ala Trp Phe Val Lys Tyr Lys Val

Asp Val Val Phe 340 345 350Ala Gly His Val His Ala Tyr Glu Arg Ser Glu Arg Val Ser Asn Ile 355 360 365Ala Tyr Asn Val Val Asn Gly Ile Cys Thr Pro Val Lys Asp Gln Ser 370 375 380Ala Pro Val Tyr Ile Thr Ile Gly Asp Gly Gly Asn Ile Glu Gly Leu385 390 395 400Ala Thr Lys Met Thr Glu Pro Gln Pro Lys Tyr Ser Ala Phe Arg Glu 405 410 415Ala Ser Phe Gly His Ala Ile Phe Ser Ile Lys Asn Arg Thr His Ala 420 425 430His Tyr Gly Trp His Arg Asn His Asp Gly Tyr Ala Val Glu Gly Asp 435 440 445Arg Met Trp Phe Tyr Asn Arg Phe Trp His Pro Val Asp Asp Ser Pro 450 455 460Ser Cys Asn Ser46542499PRTArabidopsis thaliana 42Met Leu Leu Lys Pro Gly Asn Lys Leu Val Ser Pro Glu Thr Ser His1 5 10 15His Arg Asp Ser Ala Ser Asn Ser Ser Asn His Lys Cys Gln Gln Gln 20 25 30Lys Pro Arg Lys Asp Lys Gln Lys Gln Val Glu Gln Asn Thr Lys Lys 35 40 45Ile Glu Glu His Gln Ile Lys Ser Glu Ser Thr Leu Leu Ile Ser Asn 50 55 60His Asn Val Asn Met Ser Ser Gln Ser Asn Asn Ser Glu Ser Thr Ser65 70 75 80Thr Asn Asn Ser Ser Lys Pro His Thr Gly Gly Asp Ile Arg Trp Asp 85 90 95Ala Val Asn Ser Leu Lys Ser Arg Gly Ile Lys Leu Gly Ile Ser Asp 100 105 110Phe Arg Val Leu Lys Arg Leu Gly Tyr Gly Asp Ile Gly Ser Val Tyr 115 120 125Leu Val Glu Leu Lys Gly Ala Asn Pro Thr Thr Tyr Phe Ala Met Lys 130 135 140Val Met Asp Lys Ala Ser Leu Val Ser Arg Asn Lys Leu Leu Arg Ala145 150 155 160Gln Thr Glu Arg Glu Ile Leu Ser Gln Leu Asp His Pro Phe Leu Pro 165 170 175Thr Leu Tyr Ser His Phe Glu Thr Asp Lys Phe Tyr Cys Leu Val Met 180 185 190Glu Phe Cys Ser Gly Gly Asn Leu Tyr Ser Leu Arg Gln Lys Gln Pro 195 200 205Asn Lys Cys Phe Thr Glu Asp Ala Ala Arg Phe Phe Ala Ser Glu Val 210 215 220Leu Leu Ala Leu Glu Tyr Leu His Met Leu Gly Ile Val Tyr Arg Asp225 230 235 240Leu Lys Pro Glu Asn Val Leu Val Arg Asp Asp Gly His Ile Met Leu 245 250 255Ser Asp Phe Asp Leu Ser Leu Arg Cys Ser Val Asn Pro Thr Leu Val 260 265 270Lys Ser Phe Asn Gly Gly Gly Thr Thr Gly Ile Ile Asp Asp Asn Ala 275 280 285Ala Val Gln Gly Cys Tyr Gln Pro Ser Ala Phe Phe Pro Arg Met Leu 290 295 300Gln Ser Ser Lys Lys Asn Arg Lys Ser Lys Ser Asp Phe Asp Gly Ser305 310 315 320Leu Pro Glu Leu Met Ala Glu Pro Thr Asn Val Lys Ser Met Ser Phe 325 330 335Val Gly Thr His Glu Tyr Leu Ala Pro Glu Ile Ile Lys Asn Glu Gly 340 345 350His Gly Ser Ala Val Asp Trp Trp Thr Phe Gly Ile Phe Ile Tyr Glu 355 360 365Leu Leu His Gly Ala Thr Pro Phe Lys Gly Gln Gly Asn Lys Ala Thr 370 375 380Leu Tyr Asn Val Ile Gly Gln Pro Leu Arg Phe Pro Glu Tyr Ser Gln385 390 395 400Val Ser Ser Thr Ala Lys Asp Leu Ile Lys Gly Leu Leu Val Lys Glu 405 410 415Pro Gln Asn Arg Ile Ala Tyr Lys Arg Gly Ala Thr Glu Ile Lys Gln 420 425 430His Pro Phe Phe Glu Gly Val Asn Trp Ala Leu Ile Arg Gly Glu Thr 435 440 445Pro Pro His Leu Pro Glu Pro Val Asp Phe Ser Cys Tyr Val Lys Lys 450 455 460Glu Lys Glu Ser Leu Pro Pro Ala Ala Thr Glu Lys Lys Ser Lys Met465 470 475 480Phe Asp Glu Ala Asn Lys Ser Gly Ser Asp Pro Asp Tyr Ile Val Phe 485 490 495Glu Tyr Phe431552PRTChlamydomonas reinhardtii 43Met Glu Ala Val Ala Thr Val Val Ala Asn Glu Pro Lys Val Ala Asp1 5 10 15Met Glu Glu Ile Leu Ala Glu Arg Asp Ala Cys Gly Val Gly Phe Ile 20 25 30Ala Asn Leu Lys Asn Val Gln Ser His Thr Val Val Lys Gln Ala Leu 35 40 45Thr Ala Leu Gly Cys Met Glu His Arg Gly Ala Cys Ser Ala Asp Asp 50 55 60Asp Ser Gly Asp Gly Ala Gly Leu Met Thr Gln Ile Pro Trp Lys Leu65 70 75 80Leu Lys Lys Glu Met Pro Ala Leu Asn Glu Thr Thr Thr Gly Val Gly 85 90 95Met Val Phe Met Pro Asn Asp Asp Ala Leu Glu Ala Gln Cys Lys Gln 100 105 110Ile Leu Glu Gln Val Cys Ala Lys Glu Gly Val Lys Val Val Gly Trp 115 120 125Arg Lys Val Pro Val Asn His Asp Ile Val Gly Arg Phe Ala Lys Val 130 135 140Thr Glu Pro Arg Ile Trp Gln Val Leu Ile Glu Gly Lys Ser Gly Gln145 150 155 160Val Gly Asp Glu Leu Glu Arg Glu Leu Phe Leu Val Arg Lys Leu Val 165 170 175Glu Lys Ala Lys Asn Ala Ala Leu Pro Ala Glu Phe Ala Pro Asp Phe 180 185 190Tyr Ile Cys Thr Leu Ser Ser Arg Thr Ile Val Tyr Lys Gly Met Leu 195 200 205Arg Ser Ala Val Val Gly Thr Phe Phe Arg Asp Leu Glu Asn Pro Asp 210 215 220Phe Glu Ser Ala Phe Ala Ile Tyr His Arg Arg Phe Ser Thr Asn Thr225 230 235 240Thr Pro Lys Trp Pro Leu Ala Gln Pro Met Arg Val Leu Gly His Asn 245 250 255Gly Glu Ile Asn Thr Leu Gln Gly Asn Leu Asn Trp Val Ala Ser Arg 260 265 270Glu His Glu Leu Ser Asn Pro Ile Trp Lys Gly Arg Glu Ala Glu Leu 275 280 285Thr Pro Leu Cys Asn Ala Ala Gln Ser Asp Ser Ala Asn Leu Asp Asn 290 295 300Val Ala Glu Leu Leu Val Arg Thr Gly Thr Asp Pro Gln Asp Ala Leu305 310 315 320Met Leu Leu Val Pro Glu Ala Tyr Arg Asn His Pro Asp Leu Met Lys 325 330 335Glu Tyr Pro Glu Val Val Asp Phe Tyr Glu Phe Tyr Glu Gly Leu Gln 340 345 350Glu Gly Trp Asp Gly Pro Ala Leu Leu Val Phe Ser Asp Gly Lys Arg 355 360 365Val Gly Ala Arg Leu Asp Arg Asn Gly Leu Arg Pro Ala Arg Phe Trp 370 375 380Gln Thr Lys Asp Asp Met Ile Tyr Val Ala Ser Glu Val Gly Val Leu385 390 395 400Gly Asp Ala Ile Thr Asn Ala Glu Asn Ile Val Ala Lys Gly Arg Leu 405 410 415Gly Pro Gly Gln Met Val Cys Ala Asp Leu Glu Lys Gly Ile Phe Ser 420 425 430Glu Thr Ser Ala Ile Ser Lys Leu Val Ala Gly Arg Lys Pro Tyr Lys 435 440 445Glu Trp Leu Ala Ala Ser Leu Arg Arg Leu Thr Asp Leu Gly Glu Ser 450 455 460Thr Phe Leu Asn Glu Pro Met Tyr Asp Ala Ala Thr Met Leu Arg Leu465 470 475 480Gln Ser Ala Ile Gly Met Asp Ala Glu Asn Ala Gln Met Val Val Glu 485 490 495Ser Gln Ala Gln Thr Gly Val Glu Pro Thr Tyr Cys Met Gly Asp Asp 500 505 510Ile Pro Leu Ala Val Leu Ser Asp Lys Pro His Met Leu Tyr Asp Tyr 515 520 525Phe Lys Gln Arg Phe Ala Gln Val Thr Asn Pro Pro Ile Asp Pro Leu 530 535 540Arg Glu Gly Leu Val Met Ser Leu Glu Met Arg Leu Gly Ala Arg Gly545 550 555 560Asn Leu Leu Asn Pro Gly Ala Asp Ser Tyr Lys Gln Val Leu Leu Asp 565 570 575Ser Pro Ile Leu Leu Glu Ser Glu Met Gln Ala Ile Ser Thr Asp Lys 580 585 590Val Leu Gly Ser Lys Thr Phe Lys Leu Phe Phe Glu Ala Gly Lys Pro 595 600 605Gly Ala Met Glu Ala Ala Leu Lys Lys Leu Cys Ser Asp Val Glu Ala 610 615 620Ala Val Lys Ala Gly Cys Gln Cys Val Val Leu Ser Asp Arg Pro Asp625 630 635 640Gly Gly Met Asp Ala Gly Lys Ala Pro Ile Pro Ala Leu Leu Ala Thr 645 650 655Gly Ala Val His His His Leu Ile Arg Thr Ser Leu Arg Ser Asp Thr 660 665 670Ser Ile Val Val Asp Thr Ala Thr Cys Tyr Ser Thr His His Ala Ala 675 680 685Met Leu Ile Gly Phe Gly Ala His Ala Ile Cys Pro Tyr Leu Gly Tyr 690 695 700Glu Thr Ser Arg Gln Trp Arg Leu Ser Ala Arg Thr Gln Ser Leu Ile705 710 715 720Lys Ala Gly Lys Val Pro Asp Ile Ser Val Lys Val Ala Gln Lys Asn 725 730 735Phe Lys Lys Ser Leu Glu Lys Gly Val Leu Lys Ile Leu Ser Lys Met 740 745 750Gly Ile Ser Leu Leu Ser Cys Tyr His Gly Ala Gln Ile Phe Glu Ala 755 760 765Tyr Gly Leu Gly Lys Asp Val Met Asp Met Cys Phe Lys Gly Thr Val 770 775 780Ser Arg Ile Gly Gly Met Ser Leu Ala Asp Leu Gln Arg Glu Ser Glu785 790 795 800Ser Leu Trp Ala Lys Gly Phe Pro Glu Lys Ala Met Thr Lys Leu Glu 805 810 815Asp Tyr Gly Phe Ile Gln Ser Lys Pro Lys Gly Glu Phe His Ser Asn 820 825 830Asn Gln Thr Met Ala Lys Leu Leu His Lys Ala Ile Gly Leu Gly Asn 835 840 845Gly Ser Ala Ala Asp Lys Asp Ala Tyr Lys Ala Tyr Gln Gln His Phe 850 855 860Ala Asp Ser Pro Val Ala Val Leu Arg Asp Cys Leu Glu Phe Lys Ser865 870 875 880Asp Arg Gly Pro Ile Ser Ile Asp Gln Val Glu Pro Ala Ala Ala Ile 885 890 895Met Glu Arg Phe Cys Thr Gly Gly Met Ser Leu Gly Ala Ile Ser Arg 900 905 910Glu Thr His Glu Thr Ile Ala Ile Ala Met Asn Arg Ile Gly Gly Lys 915 920 925Ser Asn Ser Gly Glu Gly Gly Glu Asp Pro Ile Arg Trp Leu His Leu 930 935 940Ser Asp Val Asp Gly Glu Gly Lys Ser Ala Thr Ala Ser Tyr Leu Arg945 950 955 960Gly Leu Arg Asn Gly Asp Thr Ala Thr Ser Lys Ile Lys Gln Val Ala 965 970 975Ser Gly Arg Phe Gly Val Thr Pro Glu Tyr Ile Met Asn Ala Glu Gln 980 985 990Met Glu Ile Lys Ile Ala Gln Gly Ala Lys Pro Gly Glu Gly Gly Gln 995 1000 1005Leu Pro Gly Gln Lys Val Ser Pro Tyr Ile Ala Gln Leu Arg Arg 1010 1015 1020Ser Lys Pro Gly Val Pro Leu Ile Ser Pro Pro Pro His His Asp 1025 1030 1035Ile Tyr Ser Ile Glu Asp Leu Ala Gln Leu Ile Tyr Asp Leu His 1040 1045 1050Gln Val Asn Pro Arg Ala Lys Val Ser Val Lys Leu Val Ala Glu 1055 1060 1065Ala Gly Ile Gly Val Val Ala Ser Gly Val Ala Lys Ala Asn Ala 1070 1075 1080Asp Ile Ile Gln Val Ser Gly His Asp Gly Gly Thr Gly Ala Ser 1085 1090 1095Pro Ile Ser Ser Ile Lys His Ala Gly Gly Pro Met Glu Met Gly 1100 1105 1110Leu Ala Glu Thr His Gln Thr Leu Val Arg Asn Glu Leu Arg Glu 1115 1120 1125Arg Val Val Leu Arg Val Asp Gly Gly Val Arg Asn Gly Arg Asp 1130 1135 1140Val Leu Met Gly Ala Leu Met Gly Ala Asp Glu Phe Gly Phe Gly 1145 1150 1155Thr Val Ala Met Ile Ala Thr Gly Cys Ile Met Ala Arg Val Cys 1160 1165 1170His Thr Asn Asn Cys Pro Val Gly Val Ala Ser Gln Arg Glu Glu 1175 1180 1185Leu Arg Ala Arg Phe Pro Gly Ala Pro Glu Asp Leu Val Asn Tyr 1190 1195 1200Phe His Phe Val Ala Glu Glu Val Arg Ala Glu Leu Ala Asn Met 1205 1210 1215Gly Tyr Arg Ser Leu Asp Glu Val Ile Gly Arg Ala Asp Leu Leu 1220 1225 1230Lys Gln Arg Ser Val Lys Leu Ala Lys Thr Glu Gly Leu Asp Leu 1235 1240 1245Ser Phe Leu Thr Thr Phe Ala Gly Ala Ser Gly Lys Ser Ser Thr 1250 1255 1260Arg Arg Ala Gln Glu Val His Asp Asn Gly Pro Gln Leu Asp Asp 1265 1270 1275Arg Ile Leu Ala Glu Pro Glu Val Met Ala Ala Ile Lys Asp His 1280 1285 1290Lys Thr Val Ser Lys Ala Phe Glu Ile Val Asn Val Asp Arg Ser 1295 1300 1305Ser Leu Gly Arg Val Ala Gly Val Ile Ala Lys His His Gly Asp 1310 1315 1320Ser Gly Phe Gln Gly Lys Val Lys Leu Thr Leu Thr Gly Ser Gly 1325 1330 1335Gly Gln Ser Phe Gly Cys Phe Cys Val Lys Gly Leu Glu Val Lys 1340 1345 1350Leu Val Gly Glu Ala Asn Asp Tyr Val Gly Lys Gly Met Asn Gly 1355 1360 1365Gly Glu Ile Ala Ile Val Pro Pro Ala Asn Ser Pro Phe Lys Pro 1370 1375 1380Glu Glu Ala Ser Leu Val Gly Asn Thr Cys Leu Tyr Gly Ala Thr 1385 1390 1395Gly Gly Arg Leu Phe Val Asn Gly Arg Ala Gly Glu Arg Phe Ala 1400 1405 1410Val Arg Asn Ser Leu Ala Glu Ala Val Val Glu Gly Ala Gly Asp 1415 1420 1425His Cys Cys Glu Tyr Met Thr Gly Gly Cys Val Ile Val Leu Gly 1430 1435 1440Ser Val Gly Arg Asn Val Ala Ala Gly Met Thr Gly Gly Leu Gly 1445 1450 1455Tyr Phe Leu Asp Glu Asp Gly Ser Phe Thr Asp Lys Val Asn Thr 1460 1465 1470Glu Ile Val Ser Val Gln Arg Val Ile Thr Lys Ala Gly Glu Ala 1475 1480 1485Gln Leu Arg Gly Leu Leu Glu Ala His Val Ala His Thr Gly Ser 1490 1495 1500Ala Lys Ala Lys Ser Leu Leu Ala Asn Trp Glu Ala Ser Leu Gly 1505 1510 1515Lys Phe Trp Gln Leu Val Pro Pro Ala Glu Lys Asn Thr Ala Glu 1520 1525 1530Val Asn Pro Ser Val Ala Gln Pro Ala Ala Ala Gly Ala Lys Val 1535 1540 1545Ala Val Ser Ala 155044449PRTArtificial SequenceChlorella sorokiniana NADP-specific glutamate dehydrogenase (NADP-GDH), N terminus residues 1 to 74 truncated 44Met His Gly Ile Lys Asn Pro Glu Leu Arg Gln Leu Leu Thr Glu Ile1 5 10 15Phe Met Lys Asp Pro Glu Gln Gln Glu Phe Met Gln Ala Val Arg Glu 20 25 30Val Ala Val Ser Leu Gln Pro Val Phe Glu Lys Arg Pro Glu Leu Leu 35 40 45Pro Ile Phe Lys Gln Ile Val Glu Pro Glu Arg Val Ile Thr Phe Arg 50 55 60Val Ser Trp Leu Asp Asp Ala Gly Asn Leu Gln Val Asn Arg Gly Phe65 70 75 80Arg Val Gln Tyr Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg 85 90 95Phe His Pro Ser Val Asn Leu Ser Ile Met Lys Phe Leu Ala Phe Glu 100 105 110Gln Ile Phe Lys Asn Ser Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 115 120 125Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val Met 130 135 140Arg Phe Cys Gln Ser Phe Met Thr Glu Leu Gln Arg His Ile Ser Tyr145 150 155 160Val Gln Asp Val Pro Ala Gly Asp Ile Gly Val Gly Ala Arg Glu Ile 165 170 175Gly Tyr Leu Phe Gly Gln Tyr Lys Arg Ile Thr Lys Asn Tyr Thr Gly 180 185 190Val Leu Thr Gly Lys Gly Gln Glu Tyr Gly Gly Ser Glu Ile Arg Pro 195 200 205Glu Ala Thr Gly Tyr Gly Ala Val Leu Phe Val Glu Asn Val Leu Lys 210 215 220Asp Lys Gly Glu Ser Leu Lys Gly Lys Arg Cys Leu Val Ser Gly Ala225 230 235 240Gly Asn Val Ala Gln Tyr Cys Ala Glu Leu Leu Leu Glu Lys Gly Ala 245 250 255Ile Val Leu Ser Leu Ser Asp Ser Gln Gly Tyr Val Tyr Glu Pro Asn

260 265 270Gly Phe Thr Arg Glu Gln Leu Gln Ala Val Gln Asp Met Lys Lys Lys 275 280 285Asn Asn Ser Ala Arg Ile Ser Glu Tyr Lys Ser Asp Thr Ala Val Tyr 290 295 300Val Gly Asp Arg Arg Lys Pro Trp Glu Leu Asp Cys Gln Val Asp Ile305 310 315 320Ala Phe Pro Cys Ala Thr Gln Asn Glu Ile Asp Glu His Asp Ala Glu 325 330 335Leu Leu Ile Lys His Gly Cys Gln Tyr Val Val Glu Gly Ala Asn Met 340 345 350Pro Ser Thr Asn Glu Ala Ile His Lys Tyr Asn Lys Ala Gly Ile Ile 355 360 365Tyr Cys Pro Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Val Ser Gly 370 375 380Leu Glu Met Thr Gln Asn Arg Met Ser Leu Asn Trp Thr Arg Glu Glu385 390 395 400Val Arg Asp Lys Leu Glu Arg Ile Met Lys Asp Ile Tyr Asp Ser Ala 405 410 415Met Gly Ala Ser Arg Glu Tyr Asn Val Asp Leu Ala Ala Gly Ala Asn 420 425 430Ile Ala Gly Phe Thr Lys Val Ala Asp Ala Val Lys Ala Gln Gly Ala 435 440 445Val45447PRTEscherichia coli 45Met Asp Gln Thr Tyr Ser Leu Glu Ser Phe Leu Asn His Val Gln Lys1 5 10 15Arg Asp Pro Asn Gln Thr Glu Phe Ala Gln Ala Val Arg Glu Val Met 20 25 30Thr Thr Leu Trp Pro Phe Leu Glu Gln Asn Pro Lys Tyr Arg Gln Met 35 40 45Ser Leu Leu Glu Arg Leu Val Glu Pro Glu Arg Val Ile Gln Phe Arg 50 55 60Val Val Trp Val Asp Asp Arg Asn Gln Ile Gln Val Asn Arg Ala Trp65 70 75 80Arg Val Gln Phe Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Met Arg 85 90 95Phe His Pro Ser Val Asn Leu Ser Ile Leu Lys Phe Leu Gly Phe Glu 100 105 110Gln Thr Phe Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 115 120 125Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Gly Glu Val Met 130 135 140Arg Phe Cys Gln Ala Leu Met Thr Glu Leu Tyr Arg His Leu Gly Ala145 150 155 160Asp Thr Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Val 165 170 175Gly Phe Met Ala Gly Met Met Lys Lys Leu Ser Asn Asn Thr Ala Cys 180 185 190Val Phe Thr Gly Lys Gly Leu Ser Phe Gly Gly Ser Leu Ile Arg Pro 195 200 205Glu Ala Thr Gly Tyr Gly Leu Val Tyr Phe Thr Glu Ala Met Leu Lys 210 215 220Arg His Gly Met Gly Phe Glu Gly Met Arg Val Ser Val Ser Gly Ser225 230 235 240Gly Asn Val Ala Gln Tyr Ala Ile Glu Lys Ala Met Glu Phe Gly Ala 245 250 255Arg Val Ile Thr Ala Ser Asp Ser Ser Gly Thr Val Val Asp Glu Ser 260 265 270Gly Phe Thr Lys Glu Lys Leu Ala Arg Leu Ile Glu Ile Lys Ala Ser 275 280 285Arg Asp Gly Arg Val Ala Asp Tyr Ala Lys Glu Phe Gly Leu Val Tyr 290 295 300Leu Glu Gly Gln Gln Pro Trp Ser Leu Pro Val Asp Ile Ala Leu Pro305 310 315 320Cys Ala Thr Gln Asn Glu Leu Asp Val Asp Ala Ala His Gln Leu Ile 325 330 335Ala Asn Gly Val Lys Ala Val Ala Glu Gly Ala Asn Met Pro Thr Thr 340 345 350Ile Glu Ala Thr Glu Leu Phe Gln Gln Ala Gly Val Leu Phe Ala Pro 355 360 365Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met 370 375 380Ala Gln Asn Ala Ala Arg Leu Gly Trp Lys Ala Glu Lys Val Asp Ala385 390 395 400Arg Leu His His Ile Met Leu Asp Ile His His Ala Cys Val Glu His 405 410 415Gly Gly Glu Gly Glu Gln Thr Asn Tyr Val Gln Gly Ala Asn Ile Ala 420 425 430Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 435 440 44546286PRTArtificial SequenceHomeobox fusion protein, Arabidopsis HB-17 N terminus residues 1 to 91 fused to soybean HB-17 C terminus residues 20-213MUTAGEN(1)..(91)source Arabidopsis HB-17 N terminus residues 1 to 91MUTAGEN(92)..(286)source soybean HB-17 C terminus residues 20-213 46Met Ile Lys Leu Leu Phe Thr Tyr Ile Cys Thr Tyr Thr Tyr Lys Leu1 5 10 15Tyr Ala Leu Tyr His Met Asp Tyr Ala Cys Val Cys Met Tyr Lys Tyr 20 25 30Lys Gly Ile Val Thr Leu Gln Val Cys Leu Phe Tyr Ile Lys Leu Arg 35 40 45Val Phe Leu Ser Asn Phe Thr Phe Ser Ser Ser Ile Leu Ala Leu Lys 50 55 60Asn Pro Asn Asn Ser Leu Ile Lys Ile Met Ala Ile Leu Pro Glu Asn65 70 75 80Ser Ser Asn Leu Asp Leu Thr Ile Ser Val Pro Gly Phe Ala Ser Ser 85 90 95Pro Thr Leu Leu Pro Ser Ser Ser Val Lys Glu Leu Asp Ile Asn Gln 100 105 110Val Pro Leu Glu Glu Asp Trp Met Ala Ser Asn Met Glu Asp Glu Glu 115 120 125Glu Ser Ser Asn Gly Glu Pro Pro Arg Lys Lys Leu Arg Leu Thr Lys 130 135 140Glu Gln Ser Leu Leu Leu Glu Glu Ser Phe Arg Gln Asn His Thr Leu145 150 155 160Asn Pro Lys Gln Lys Glu Ser Leu Ala Met Gln Leu Lys Leu Arg Pro 165 170 175Arg Gln Val Glu Val Trp Phe Gln Asn Arg Arg Ala Arg Ser Lys Leu 180 185 190Lys Gln Thr Glu Met Glu Cys Glu Tyr Leu Lys Arg Trp Phe Gly Ser 195 200 205Leu Thr Glu Gln Asn Arg Arg Leu Gln Arg Glu Val Glu Glu Leu Arg 210 215 220Ala Ile Lys Val Gly Pro Pro Thr Val Ile Ser Pro His Ser Cys Glu225 230 235 240Pro Leu Pro Ala Ser Thr Leu Ser Met Cys Pro Arg Cys Glu Arg Val 245 250 255Thr Ser Thr Ala Asp Lys Pro Pro Ser Ala Ala Ala Thr Leu Ser Ala 260 265 270Lys Val Pro Pro Thr Gln Ser Arg Gln Pro Ser Ala Ala Cys 275 280 28547345PRTGlycine max 47Met Cys Gly Gly Ala Ile Ile Ser Asp Phe Ile Pro Ala Ala Ala Ile1 5 10 15Ala Gly Ser Arg Arg Leu Thr Ala Asp Tyr Leu Trp Pro Asp Leu Lys 20 25 30Lys Arg Lys Ser Asp Leu Asp Val Asp Phe Glu Ala Asp Phe Arg Asp 35 40 45Phe Lys Asp Asp Ser Asp Ile Asp Asp Asp Asp Asp Asp His Gln Val 50 55 60Lys Pro Phe Ala Phe Ala Ala Ser Ser Arg Leu Ser Thr Ala Ala Lys65 70 75 80Ser Val Ala Phe Gln Gly Arg Ala Glu Ile Ser Ala Asn Arg Lys Arg 85 90 95Lys Asn Gln Tyr Arg Gly Ile Arg Gln Arg Pro Trp Gly Lys Trp Ala 100 105 110Ala Glu Ile Arg Asp Pro Arg Lys Gly Val Arg Val Trp Leu Gly Thr 115 120 125Phe Asn Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Ala Glu Ala Arg 130 135 140Arg Ile Arg Gly Lys Lys Ala Lys Val Asn Phe Pro Glu Ala Pro Gly145 150 155 160Thr Ser Ser Val Lys Arg Ser Lys Val Asn Pro Gln Glu Asn Leu Lys 165 170 175Thr Val Gln Pro Asn Leu Gly His Lys Phe Ser Ala Gly Asn Asn His 180 185 190Met Asp Leu Val Glu Gln Lys Pro Leu Val Ser Gln Tyr Ala Asn Met 195 200 205Ala Ser Phe Pro Gly Ser Gly Asn Gly Leu Arg Ser Leu Pro Ser Ser 210 215 220Asp Asp Ala Thr Leu Tyr Phe Ser Ser Asp Gln Gly Ser Asn Ser Phe225 230 235 240Asp Tyr Ala Pro Glu Ile Ser Ser Met Leu Ser Ala Pro Leu Asp Cys 245 250 255Glu Ser His Phe Val Gln Asn Ala Asn Gln Gln Gln Pro Asn Ser Gln 260 265 270Asn Val Val Ser Ile Glu Asp Asp Ser Ala Lys Thr Leu Ser Glu Glu 275 280 285Leu Val Asp Ile Glu Ser Glu Leu Lys Phe Phe Gln Met Pro Tyr Leu 290 295 300Glu Gly Ser Trp Gly Asp Thr Ser Leu Glu Ser Leu Leu Ser Gly Asp305 310 315 320Thr Thr Gln Asp Gly Gly Asn Leu Met Asn Leu Trp Cys Phe Asp Asp 325 330 335Ile Pro Ser Met Ala Gly Gly Val Phe 340 34548257PRTOryza sativa 48Met Ser Met Gly Pro Ala Ala Gly Glu Gly Cys Gly Leu Cys Gly Ala1 5 10 15Asp Gly Gly Gly Cys Cys Ser Arg His Arg His Asp Asp Asp Gly Phe 20 25 30Pro Phe Val Phe Pro Pro Ser Ala Cys Gln Gly Ile Gly Ala Pro Ala 35 40 45Pro Pro Val His Glu Phe Gln Phe Phe Gly Asn Asp Gly Gly Gly Asp 50 55 60Asp Gly Glu Ser Val Ala Trp Leu Phe Asp Asp Tyr Pro Pro Pro Ser65 70 75 80Pro Val Ala Ala Ala Ala Gly Met His His Arg Gln Pro Pro Tyr Asp 85 90 95Gly Val Val Ala Pro Pro Ser Leu Phe Arg Arg Asn Thr Gly Ala Gly 100 105 110Gly Leu Thr Phe Asp Val Ser Leu Gly Gly Arg Pro Asp Leu Asp Ala 115 120 125Gly Leu Gly Leu Gly Gly Gly Ser Gly Arg His Ala Glu Ala Ala Ala 130 135 140Ser Ala Thr Ile Met Ser Tyr Cys Gly Ser Thr Phe Thr Asp Ala Ala145 150 155 160Ser Ser Met Pro Lys Glu Met Val Ala Ala Met Ala Asp Val Gly Glu 165 170 175Ser Leu Asn Pro Asn Thr Val Val Gly Ala Met Val Glu Arg Glu Ala 180 185 190Lys Leu Met Arg Tyr Lys Glu Lys Arg Lys Lys Arg Cys Tyr Glu Lys 195 200 205Gln Ile Arg Tyr Ala Ser Arg Lys Ala Tyr Ala Glu Met Arg Pro Arg 210 215 220Val Arg Gly Arg Phe Ala Lys Glu Ala Asp Gln Glu Ala Val Ala Pro225 230 235 240Pro Ser Thr Tyr Val Asp Pro Ser Arg Leu Glu Leu Gly Gln Trp Phe 245 250 255Arg49971PRTZea mays 49Met Ile Val Gln Pro Ile Glu Leu Arg Ala Trp Thr Ala Phe Pro Gly1 5 10 15Ser Ala Gln Glu Gly Ile Gly Arg Met Ala Ala Ser Val Ser Arg Ala 20 25 30Ile Cys Val Gln Lys Pro Gly Ser Lys Cys Thr Arg Asp Arg Glu Ala 35 40 45Thr Ser Phe Ala Arg Arg Ser Val Ala Ala Pro Arg Pro Pro His Ala 50 55 60Lys Ala Ala Gly Val Ile Arg Ser Asp Ser Gly Ala Gly Arg Gly Gln65 70 75 80His Cys Ser Pro Leu Arg Ala Val Val Asp Ala Ala Pro Ile Gln Thr 85 90 95Thr Lys Lys Arg Val Phe His Phe Gly Lys Gly Lys Ser Glu Gly Asn 100 105 110Lys Thr Met Lys Glu Leu Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu 115 120 125Met Ala Ser Ile Gly Leu Ser Val Pro Pro Gly Phe Thr Val Ser Thr 130 135 140Glu Ala Cys Gln Gln Tyr Gln Asp Ala Gly Cys Ala Leu Pro Ala Gly145 150 155 160Leu Trp Ala Glu Ile Val Asp Gly Leu Gln Trp Val Glu Glu Tyr Met 165 170 175Gly Ala Thr Leu Gly Asp Pro Gln Arg Pro Leu Leu Leu Ser Val Arg 180 185 190Ser Gly Ala Ala Val Ser Met Pro Gly Met Met Asp Thr Val Leu Asn 195 200 205Leu Gly Leu Asn Asp Glu Val Ala Ala Gly Leu Ala Ala Lys Ser Gly 210 215 220Glu Arg Phe Ala Tyr Asp Ser Phe Arg Arg Phe Leu Asp Met Phe Gly225 230 235 240Asn Val Val Met Asp Ile Pro Arg Ser Leu Phe Glu Glu Lys Leu Glu 245 250 255His Met Lys Glu Ser Lys Gly Leu Lys Asn Asp Thr Asp Leu Thr Ala 260 265 270Ser Asp Leu Lys Glu Leu Val Gly Gln Tyr Lys Glu Val Tyr Leu Ser 275 280 285Ala Lys Gly Glu Pro Phe Pro Ser Asp Pro Lys Lys Gln Leu Glu Leu 290 295 300Ala Val Leu Ala Val Phe Asn Ser Trp Glu Ser Pro Arg Ala Lys Lys305 310 315 320Tyr Arg Ser Ile Asn Gln Ile Thr Gly Leu Arg Gly Thr Ala Val Asn 325 330 335Val Gln Cys Met Val Phe Gly Asn Met Gly Asn Thr Ser Gly Thr Gly 340 345 350Val Leu Phe Thr Arg Asn Pro Asn Thr Gly Glu Lys Lys Leu Tyr Gly 355 360 365Glu Phe Leu Val Asn Ala Gln Gly Glu Asp Val Val Ala Gly Ile Arg 370 375 380Thr Pro Glu Asp Leu Asp Ala Met Lys Asn Leu Met Pro Gln Ala Tyr385 390 395 400Asp Glu Leu Val Glu Asn Cys Asn Ile Leu Glu Ser His Tyr Lys Glu 405 410 415Met Gln Asp Ile Glu Phe Thr Val Gln Glu Asn Arg Leu Trp Met Leu 420 425 430Gln Cys Arg Thr Gly Lys Arg Thr Gly Lys Ser Ala Val Lys Ile Ala 435 440 445Val Asp Met Val Asn Glu Gly Leu Val Glu Pro Arg Ser Ala Ile Lys 450 455 460Met Val Glu Pro Gly His Leu Asp Gln Leu Leu His Pro Gln Phe Glu465 470 475 480Asn Pro Ser Ala Tyr Lys Asp Gln Val Ile Ala Thr Gly Leu Pro Ala 485 490 495Ser Pro Gly Ala Ala Val Gly Gln Val Val Phe Thr Ala Glu Asp Ala 500 505 510Glu Ala Trp His Ser Gln Gly Lys Ala Ala Ile Leu Val Arg Ala Glu 515 520 525Thr Ser Pro Glu Asp Val Gly Gly Met His Ala Ala Val Gly Ile Leu 530 535 540Thr Glu Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg Gly545 550 555 560Trp Gly Lys Cys Cys Val Ser Gly Cys Ser Gly Ile Arg Val Asn Asp 565 570 575Ala Glu Lys Leu Val Thr Ile Gly Gly His Val Leu Arg Glu Gly Glu 580 585 590Trp Leu Ser Leu Asn Gly Ser Thr Gly Glu Val Ile Leu Gly Lys Gln 595 600 605Pro Leu Ser Pro Pro Ala Leu Ser Gly Asp Leu Gly Thr Phe Met Ala 610 615 620Trp Val Asp Asp Val Arg Lys Leu Lys Val Leu Ala Asn Ala Asp Thr625 630 635 640Pro Asp Asp Ala Leu Thr Ala Arg Asn Asn Gly Ala Gln Gly Ile Gly 645 650 655Leu Cys Arg Thr Glu His Met Phe Phe Ala Ser Asp Glu Arg Ile Lys 660 665 670Ala Val Arg Gln Met Ile Met Ala Pro Thr Leu Glu Leu Arg Gln Gln 675 680 685Ala Leu Asp Arg Leu Leu Pro Tyr Gln Arg Ser Asp Phe Glu Gly Ile 690 695 700Phe Arg Ala Met Asp Gly Leu Pro Val Thr Ile Arg Leu Leu Asp Pro705 710 715 720Pro Leu His Glu Phe Leu Pro Glu Gly Asn Ile Glu Asp Ile Val Ser 725 730 735Glu Leu Cys Ala Glu Thr Gly Ala Asn Gln Glu Asp Ala Leu Ala Arg 740 745 750Ile Glu Lys Leu Ser Glu Val Asn Pro Met Leu Gly Phe Arg Gly Cys 755 760 765Arg Leu Gly Ile Ser Tyr Pro Glu Leu Thr Glu Met Gln Ala Arg Ala 770 775 780Ile Phe Glu Ala Ala Ile Ala Met Thr Asn Gln Gly Val Gln Val Phe785 790 795 800Pro Glu Ile Met Val Pro Leu Val Gly Thr Pro Gln Glu Leu Gly His 805 810 815Gln Val Thr Leu Ile Arg Gln Val Ala Glu Lys Val Phe Ala Asn Val 820 825 830Gly Lys Thr Ile Gly Tyr Lys Val Gly Thr Met Ile Glu Ile Pro Arg 835 840 845Ala Ala Leu Val Ala Asp Glu Ile Ala Glu Gln Ala Glu Phe Phe Ser 850 855 860Phe Gly Thr Asn Asp Leu Thr Gln Met Thr Phe Gly Tyr Ser Arg Asp865 870 875 880Asp Val Gly Lys Phe Ile Pro Val Tyr Pro Ala Gln Gly Ile Leu Gln 885 890 895His Asp Pro Phe Glu Val Leu Asp Gln Arg Gly Val

Gly Glu Leu Val 900 905 910Lys Phe Ala Thr Glu Arg Gly Arg Lys Ala Arg Pro Asn Leu Lys Val 915 920 925Gly Ile Cys Gly Glu His Gly Gly Glu Pro Ser Ser Val Ala Phe Phe 930 935 940Ala Lys Ala Gly Leu Asp Tyr Val Ser Cys Ser Pro Phe Arg Val Pro945 950 955 960Ile Ala Arg Leu Ala Ala Ala Gln Val Leu Val 965 97050263PRTZea mays 50Met Leu Glu Leu Arg Leu Val Gln Gly Ser Leu Leu Lys Lys Val Leu1 5 10 15Glu Ala Ile Arg Glu Leu Val Thr Asp Ala Asn Phe Asp Cys Ser Gly 20 25 30Thr Gly Phe Ser Leu Gln Ala Met Asp Ser Ser His Val Ala Leu Val 35 40 45Ala Leu Leu Leu Arg Ala Glu Gly Phe Glu His Tyr Arg Cys Asp Arg 50 55 60Asn Leu Ser Met Gly Met Asn Leu Asn Asn Met Ala Lys Met Leu Arg65 70 75 80Cys Ala Gly Asn Glu Asp Ile Ile Thr Ile Lys Ala Asp Asp Gly Ser 85 90 95Asp Thr Val Thr Phe Met Phe Glu Ser Pro Lys Gln Asp Lys Ile Ala 100 105 110Asp Phe Glu Met Lys Leu Met Asp Ile Asp Ser Glu His Leu Gly Ile 115 120 125Pro Asp Ser Glu Tyr Gln Ala Ile Val Arg Met Pro Ser Ala Glu Phe 130 135 140Met Arg Ile Cys Lys Asp Leu Ser Ser Ile Gly Asp Thr Val Val Ile145 150 155 160Ser Val Thr Lys Glu Gly Val Lys Phe Ser Thr Ser Gly Glu Ile Gly 165 170 175Ser Ala Asn Ile Val Cys Arg Gln Asn Gln Thr Ile Asp Lys Pro Glu 180 185 190Glu Ala Thr Ile Ile Glu Met Gln Glu Pro Val Ser Leu Thr Phe Ala 195 200 205Leu Arg Tyr Met Asn Ser Phe Thr Lys Ala Ser Ser Leu Ser Glu Gln 210 215 220Val Thr Ile Ser Leu Ser Ser Glu Leu Pro Val Val Val Glu Tyr Lys225 230 235 240Ile Ala Glu Met Gly Tyr Ile Arg Phe Tyr Leu Ala Pro Lys Ile Asp 245 250 255Asp Asp Glu Glu Met Lys Pro 26051889PRTZea mays 51Met Ala Lys Ser Ser Ala Asp Asp Ala Glu Leu Arg Arg Ala Cys Ala1 5 10 15Ala Ala Val Ala Ala Ser Gly Ala Arg Gly Glu Glu Val Ala Phe Ser 20 25 30Ile Arg Val Ala Lys Gly Arg Gly Ile Phe Glu Lys Leu Gly Arg Leu 35 40 45Ala Lys Pro Arg Val Leu Ala Leu Thr Val Lys Gln Ser Ser Arg Gly 50 55 60Glu Ala Asn Lys Ala Phe Leu Arg Val Leu Lys Tyr Ser Ser Gly Ala65 70 75 80Val Leu Glu Pro Ala Lys Leu Tyr Lys Leu Lys His Leu Thr Lys Val 85 90 95Glu Val Ile Ser Asn Asp Pro Ser Gly Cys Thr Phe Val Leu Gly Phe 100 105 110Asp Asn Leu Arg Ser Gln Ser Val Ala Pro Pro Gln Trp Thr Met Arg 115 120 125Asn Ile Asp Asp Arg Asn Arg Leu Leu Phe Cys Ile Leu Asn Met Cys 130 135 140Lys Glu Ile Leu Ser Tyr Leu Pro Lys Val Val Gly Ile Asp Ile Val145 150 155 160Glu Leu Ala Leu Trp Ala Lys Glu Asn Thr Leu Thr Ile Asp Asn Gln 165 170 175Val Ser Thr Gln Asp Gly Gln Glu Thr Ser Val Ala Thr Gln Thr Glu 180 185 190Arg Lys Val Thr Val Thr Val Glu Asn Asp Leu Val Ser Gln Ala Lys 195 200 205Glu Glu Glu Glu Asp Met Glu Ala Leu Leu Asp Thr Tyr Val Met Gly 210 215 220Ile Gly Glu Ala Asp Ala Phe Ser Glu Arg Leu Lys Gln Glu Leu Val225 230 235 240Ala Leu Glu Ala Ala Asn Val Tyr Gln Leu Leu Glu Ser Glu Pro Leu 245 250 255Ile Glu Glu Val Leu Gln Gly Leu Asp Ala Ala Ser Ala Thr Val Asp 260 265 270Asp Met Asp Glu Trp Leu Arg Ile Phe Asn Leu Lys Leu Arg His Met 275 280 285Arg Glu Asp Ile Ala Ser Ile Glu Ser Arg Asn Asn Gly Leu Glu Met 290 295 300Gln Ser Val Asn Asn Lys Gly Leu Met Glu Glu Leu Asp Lys Leu Leu305 310 315 320Glu Arg Leu Arg Ile Pro Gln Glu Phe Ala Ala Ser Leu Thr Gly Gly 325 330 335Ser Phe Glu Glu Ser Arg Met Leu Lys Asn Val Glu Ala Cys Glu Trp 340 345 350Leu Thr Gly Ala Ile Arg Ser Leu Glu Val Pro Asn Leu Asp Pro Cys 355 360 365Tyr Val Asn Met Arg Ala Val Arg Glu Lys Lys Ala Glu Leu Glu Lys 370 375 380Leu Lys Thr Thr Phe Val Arg Arg Ala Ser Glu Phe Leu Arg Asn Tyr385 390 395 400Phe Ser Ser Leu Val Asp Phe Met Ile Ser Asp Lys Ser Tyr Phe Ser 405 410 415Gln Arg Gly Gln Leu Lys Arg Pro Asp His Ala Asp Leu Arg Tyr Lys 420 425 430Cys Arg Thr Tyr Ala Arg Leu Leu Gln His Leu Lys Ser Leu Asp Lys 435 440 445Ser Cys Leu Gly Pro Leu Arg Lys Ala Tyr Cys His Ser Leu Asn Leu 450 455 460Leu Leu Arg Arg Glu Ala Arg Glu Phe Ala Asn Glu Leu Arg Ala Ser465 470 475 480Thr Lys Ala Pro Lys Asn Pro Ala Val Trp Leu Glu Gly Ser Gly Gly 485 490 495Ser Gly His Asn Gly Ser Ser Ser Asp Thr Ser Gln Val Ser Asp Ala 500 505 510Tyr Ser Lys Met Leu Thr Ile Phe Ile Pro Leu Leu Val Asp Glu Ser 515 520 525Ser Phe Phe Ala His Phe Met Cys Phe Glu Val Pro Ala Leu Val Pro 530 535 540Ala Gly Ser Pro Asn Ala Asn Lys Ser Lys Ser Gly Gly Asn Asp Pro545 550 555 560Asp Asp Asp Leu Gly Leu Met Asp Pro Asp Gly Asn Asp Leu Lys Pro 565 570 575Asp Ser Thr Ser Ala Glu Leu Gly Thr Leu Asn Glu Ala Leu Gln Glu 580 585 590Leu Leu Asp Gly Ile Gln Glu Asp Phe Tyr Ala Val Val Asp Trp Ala 595 600 605Tyr Lys Ile Asp Pro Leu Arg Cys Ile Ser Met His Gly Ile Thr Glu 610 615 620Arg Tyr Leu Ser Gly Gln Lys Ala Asp Ala Ala Gly Phe Val Arg Lys625 630 635 640Leu Leu Asp Asp Leu Glu Ser Arg Ile Ser Val Gln Phe Ser Arg Phe 645 650 655Ile Asp Glu Ala Cys His Gln Ile Glu Arg Asn Glu Arg Asn Val Arg 660 665 670Gln Thr Gly Ile Leu Ala Tyr Ile Pro Arg Phe Ala Val Leu Ala Ser 675 680 685Arg Met Glu Gln Tyr Ile Gln Gly Gln Ser Arg Asp Leu Ile Asp Lys 690 695 700Ala Tyr Thr Lys Leu Val Ser Thr Met Phe Ala Thr Leu Glu Lys Ile705 710 715 720Ala Gln Ser Asp Pro Lys Thr Ala Asp Ile Val Leu Ile Glu Asn Tyr 725 730 735Ala Ala Phe Gln Asn Ser Leu Tyr Asp Leu Ala Asn Val Val Pro Thr 740 745 750Leu Ala Lys Phe Tyr His Gln Ala Ser Glu Ser Tyr Glu Leu Ala Cys 755 760 765Thr Arg His Ile Ser Ser Leu Ile Tyr Leu Gln Phe Glu Arg Leu Phe 770 775 780Gln Phe Asn Arg Lys Val Asp Glu Leu Thr Tyr Thr Ile Ala Ala Glu785 790 795 800Glu Ile Pro Phe Gln Leu Gly Leu Ser Lys Thr Asp Leu Arg Arg Val 805 810 815Leu Lys Ser Ser Leu Ser Gly Ile Asp Lys Ser Ile Ser Ala Met Tyr 820 825 830Arg Arg Leu Gln Lys Thr Leu Thr Ser Asp Glu Leu Phe Pro Ser Leu 835 840 845Trp Asp Lys Cys Lys Lys Glu Phe Leu Asp Lys Tyr Glu Ser Phe Val 850 855 860Gln Met Val Thr Arg Ile Tyr Gly Asn Glu Pro Ile Met Ser Val Asn865 870 875 880Glu Met Lys Asp Val Leu Ala Gly Phe 88552392PRTZea mays 52Met Gly Thr Gln Ile Val Arg Glu Gln Thr Ser Gly Ser Leu Thr Ala1 5 10 15Ile Val Val Asp Glu Asn Leu Cys His Ala Arg Ala Ala Ser Cys Met 20 25 30Leu Ala Asn Leu Gln Cys Lys Val Ile Val Tyr Ala Ser Pro Val Asp 35 40 45Ala Leu Lys Phe Leu Lys Asp His Gln Arg Asp Thr Asp Phe Ala Leu 50 55 60Val Glu Val Asn Met Lys Glu Met His Gly Phe Gln Phe Leu Asp Met65 70 75 80Ser Arg Lys Leu His Lys Ser Leu Gln Val Ile Met Met Ser Ala Asp 85 90 95Thr Thr Trp Pro Thr Met Lys Arg Ser Val Glu Leu Gly Ala Arg Phe 100 105 110Leu Ile Lys Lys Pro Leu Asp Ala Asn Thr Met Asn Asn Leu Trp Gln 115 120 125His Leu Asp Leu Lys Phe Gln Arg Thr Asp Lys Ile Lys Ala Leu Phe 130 135 140Pro Gly Ile Glu Gly Lys Thr Gly Asn Ala Phe Glu Glu Gly Thr Asn145 150 155 160Lys Gln Lys Gly Thr His Leu Met Trp Thr Pro Phe Leu Gln Arg Lys 165 170 175Phe Leu Gln Ala Val Glu Leu Leu Gly Glu Asp Ala Ser Pro Lys Lys 180 185 190Ile Gln Leu Leu Met Asn Val Asn Ser Val Ser Arg Lys Gln Ile Ser 195 200 205Ala His Leu Gln Lys His Arg Lys Lys Val Glu Lys Glu Leu Arg Asn 210 215 220Ser Asn Ala Asn Asn Ser Ser His Gly Ile Gly Gly Ala Ser Asn Ser225 230 235 240Arg Pro Ser Arg Ile Phe Glu Ile Ser His Gly Arg Phe Gln Tyr Asn 245 250 255Arg Pro Asp Val Gln Pro Glu His Arg Ser Asp Glu Ser Val Ser Val 260 265 270Glu Gln Thr Glu Thr Ile Glu Glu Thr Gln Ser Asn Arg Leu Tyr Glu 275 280 285Ala Met Arg Arg Ala Leu Gln Leu Gly Ser Val Phe Glu Glu Pro Gln 290 295 300Leu Pro Asn Asp Pro Pro Ala Gly Lys Asp Ala Arg Glu Val Glu Glu305 310 315 320Val Glu Met Thr Met Arg Asp Gly Asn Tyr Arg Asp Ala Gly Thr Asp 325 330 335Ala Phe Gly Asp Lys Asn Glu Ala Ser Gly Thr His Ser Ser Asp Gly 340 345 350Asn Asn Ala Lys Val Lys Ser Lys Asp Asp Ser Ala Asp Lys Leu Val 355 360 365Ser Cys His Asp Glu Leu Arg Pro Val Val Thr Leu Val Thr Tyr Ser 370 375 380Asp Ser Glu Asp Gly Glu Thr Leu385 39053255PRTZea mays 53Met Gly Arg Gly Arg Val Glu Leu Lys Arg Ile Glu Asn Lys Ile Asn1 5 10 15Arg Gln Val Thr Phe Ser Lys Arg Arg Asn Gly Leu Leu Lys Lys Ala 20 25 30Tyr Glu Leu Ser Val Leu Cys Asp Ala Glu Val Ala Leu Ile Ile Phe 35 40 45Ser Ser Arg Gly Lys Leu Tyr Glu Phe Gly Ser Ala Gly Ile Thr Lys 50 55 60Thr Leu Glu Arg Tyr Gln His Cys Cys Tyr Asn Ala Gln Asp Ser Asn65 70 75 80Gly Ala Leu Ser Glu Thr Gln Ser Trp Tyr Gln Glu Met Ser Lys Leu 85 90 95Arg Ala Lys Phe Glu Ala Leu Gln Arg Thr Gln Arg His Leu Leu Gly 100 105 110Glu Glu Leu Gly Pro Leu Ser Val Lys Glu Leu Gln Gln Leu Glu Lys 115 120 125Gln Leu Glu Cys Ala Leu Ser Gln Ala Arg Gln Arg Lys Thr Gln Leu 130 135 140Met Met Glu Gln Val Glu Glu Leu Arg Arg Lys Glu Arg His Leu Gly145 150 155 160Glu Met Asn Arg Gln Leu Lys His Lys Leu Glu Ala Glu Gly Cys Ser 165 170 175Asn Tyr Arg Thr Leu Gln His Ala Ala Trp Pro Ala Pro Gly Ser Thr 180 185 190Met Val Glu His Asp Gly Ala Thr Tyr His Val His Pro Thr Thr Ala 195 200 205Gln Ser Val Ala Met Asp Cys Glu Pro Thr Leu Gln Ile Gly Tyr Pro 210 215 220Pro His His Gln Phe Leu Pro Ser Glu Ala Ala Asn Asn Ile Pro Arg225 230 235 240Ser Pro Pro Gly Gly Glu Asn Asn Phe Met Leu Gly Trp Val Leu 245 250 25554199PRTZea mays 54Met Ala Glu Glu Glu Ala Lys Lys Val Glu Val Glu Val Thr Lys Glu1 5 10 15Pro Glu Ala Ala Ala Lys Glu Asp Val Ala Asp Asp Lys Ala Val Ile 20 25 30Pro Ala Thr Asp Pro Pro Pro Pro Pro Pro Pro Ala Asp Asp Ser Lys 35 40 45Ala Leu Ala Ile Val Glu Lys Val Ala Asp Glu Pro Ala Pro Glu Lys 50 55 60Pro Ala Pro Ala Lys Gln Gly Gly Ser Asn Asp Arg Asp Leu Ala Leu65 70 75 80Ala Arg Val Glu Thr Glu Lys Arg Asn Ser Leu Ile Lys Ala Trp Glu 85 90 95Glu Asn Glu Lys Thr Lys Ala Glu Asn Lys Ala Ala Lys Lys Val Ser 100 105 110Ala Ile Leu Ser Trp Glu Asn Thr Lys Lys Ala Asn Ile Glu Ala Glu 115 120 125Leu Lys Lys Ile Glu Glu Gln Leu Glu Lys Lys Lys Ala Glu Tyr Ala 130 135 140Glu Lys Met Lys Asn Lys Val Ala Met Ile His Lys Glu Ala Glu Glu145 150 155 160Lys Arg Ala Met Val Glu Ala Lys Arg Gly Glu Glu Val Leu Lys Ala 165 170 175Glu Glu Met Ala Ala Lys Tyr Arg Ala Thr Gly His Ala Pro Lys Lys 180 185 190Leu Ile Gly Cys Phe Gly Ala 19555178PRTZea mays 55Met Ala Glu Ala Pro Ala Ser Pro Gly Gly Gly Gly Gly Ser His Glu1 5 10 15Ser Gly Ser Pro Arg Gly Gly Gly Gly Gly Gly Ser Val Arg Glu Gln 20 25 30Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala 35 40 45Ile Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln 50 55 60Glu Cys Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala Ser Asp65 70 75 80Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu 85 90 95Trp Ala Met Ala Thr Leu Gly Phe Glu Asp Tyr Ile Glu Pro Leu Lys 100 105 110Val Tyr Leu Gln Lys Tyr Arg Glu Met Glu Gly Asp Ser Lys Leu Thr 115 120 125Ala Lys Ser Ser Asp Gly Ser Ile Lys Lys Asp Ala Leu Gly His Val 130 135 140Gly Ala Ser Ser Ser Ala Ala Gln Gly Met Gly Gln Gln Gly Ala Tyr145 150 155 160Asn Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His Asn Gly Asp Ile 165 170 175Ser Asn56213PRTZea mays 56Met Ser Ser Gly Ser Gly Ser Gly Asn Lys Arg Ala Ala Ala Glu Arg1 5 10 15Ser Ala Gly Ala Gly Ala Gly Ser Gly Asp Glu Asp Asp Asp Gly Ala 20 25 30Ala Arg Lys Lys Leu Arg Leu Ser Lys Asp Gln Ala Ala Val Leu Glu 35 40 45Glu Cys Phe Lys Thr His His Thr Leu Thr Pro Lys Gln Lys Val Ala 50 55 60Leu Ala Ser Ser Leu Gly Leu Arg Pro Arg Gln Val Glu Val Trp Phe65 70 75 80Gln Asn Arg Arg Ala Arg Thr Lys Leu Lys Gln Thr Glu Val Asp Cys 85 90 95Glu Tyr Leu Lys Arg Trp Cys Glu Gln Leu Ala Glu Glu Asn Arg Arg 100 105 110Leu Gly Lys Glu Val Ala Glu Leu Arg Ala Leu Ser Ala Ala Pro Ala 115 120 125Ala Pro Leu Thr Thr Leu Thr Met Cys Leu Ser Cys Arg Arg Val Ala 130 135 140Ser Ser Ser Pro Ser Ser Ser Ser Ser Pro Arg Pro Ser Ile Pro Gly145 150 155 160Ala Ala Ala Ala Ser Gly Gly Ser Met Ala Ser Pro Ala Ala Ala Ala 165 170 175Thr Leu Pro Ala His Arg Gln Phe Phe Cys Gly Phe Arg Asp Ala Gly 180 185 190Ala Ala Ala Ala Ala Tyr Gly Thr Ala Ser Ala Gly Leu Ala Lys Pro 195 200 205Val Arg Ala Ala Arg 21057423PRTArtificial Sequencecorn putative glutamine synthetase (with C299A

mutation)MUTAGEN(299)..(299)C299A mutation 57Met Ala Gln Ala Val Val Pro Ala Met Gln Cys Arg Val Gly Val Lys1 5 10 15Ala Ala Ala Gly Arg Val Trp Ser Ala Gly Arg Thr Arg Thr Gly Arg 20 25 30Gly Gly Ala Ser Pro Gly Phe Lys Val Met Ala Val Ser Thr Gly Ser 35 40 45Thr Gly Val Val Pro Arg Leu Glu Gln Leu Leu Asn Met Asp Thr Thr 50 55 60Pro Tyr Thr Asp Lys Val Ile Ala Glu Tyr Ile Trp Val Gly Gly Ser65 70 75 80Gly Ile Asp Ile Arg Ser Lys Ser Arg Thr Ile Ser Lys Pro Val Glu 85 90 95Asp Pro Ser Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly 100 105 110Gln Ala Pro Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile 115 120 125Phe Lys Asp Pro Phe Arg Gly Gly Asn Asn Val Leu Val Ile Cys Asp 130 135 140Thr Tyr Thr Pro Gln Gly Glu Pro Leu Pro Thr Asn Lys Arg His Arg145 150 155 160Ala Ala Gln Ile Phe Ser Asp Pro Lys Val Ala Glu Gln Val Pro Trp 165 170 175Phe Gly Ile Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Asn Trp 180 185 190Pro Leu Gly Trp Pro Val Gly Gly Phe Pro Gly Pro Gln Gly Pro Tyr 195 200 205Tyr Cys Ala Val Gly Ala Asp Lys Ser Phe Gly Arg Asp Ile Ser Asp 210 215 220Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Thr225 230 235 240Asn Gly Glu Val Met Pro Gly Gln Trp Glu Tyr Gln Val Gly Pro Ser 245 250 255Val Gly Ile Glu Ala Gly Asp His Ile Trp Ile Ser Arg Tyr Ile Leu 260 265 270Glu Arg Ile Thr Glu Gln Ala Gly Val Val Leu Thr Leu Asp Pro Lys 275 280 285Pro Ile Gln Gly Asp Trp Asn Gly Ala Gly Ala His Thr Asn Tyr Ser 290 295 300Thr Lys Thr Met Arg Glu Asp Gly Gly Phe Glu Glu Ile Lys Arg Ala305 310 315 320Ile Leu Asn Leu Ser Leu Arg His Asp Leu His Ile Ser Ala Tyr Gly 325 330 335Glu Gly Asn Glu Arg Arg Leu Thr Gly Lys His Glu Thr Ala Ser Ile 340 345 350Gly Thr Phe Ser Trp Gly Val Ala Asn Arg Gly Cys Ser Ile Arg Val 355 360 365Gly Arg Asp Thr Glu Ala Lys Gly Lys Gly Tyr Leu Glu Asp Arg Arg 370 375 380Pro Ala Ser Asn Met Asp Pro Tyr Ile Val Thr Gly Leu Leu Ala Glu385 390 395 400Thr Thr Ile Leu Trp Gln Pro Ser Leu Glu Ala Glu Ala Leu Ala Ala 405 410 415Lys Lys Leu Ala Leu Lys Val 42058594PRTZea mays 58Met Val Lys Arg Ser Lys Lys Ser Lys Ser Lys Arg Val Thr Leu Arg1 5 10 15Gln Lys His Lys Val Gln Arg Lys Val Lys Glu His His Arg Lys Lys 20 25 30Arg Lys Glu Ala Lys Lys Ala Gly Lys Ala Gly Gln Arg Arg Lys Val 35 40 45Glu Lys Asp Pro Gly Ile Pro Asn Glu Trp Pro Phe Lys Glu Gln Glu 50 55 60Leu Lys Ala Leu Glu Ala Arg Arg Ala Gln Ala Leu Gln Glu Leu Glu65 70 75 80Leu Lys Lys Gln Ala Arg Lys Glu Arg Ala Gln Lys Arg Lys Ala Gly 85 90 95Leu Leu Glu Asp Glu Asp Ile Ala Ser Leu Ala Ser Ala Ala Ser Ala 100 105 110Gln Gly Ser Glu Phe Ala Ala Lys Glu Asn Ala Pro Leu Leu Val Ala 115 120 125Lys Ile Asn Asp His Ser Glu Arg Ser Phe Tyr Lys Glu Leu Val Lys 130 135 140Val Ile Glu Ala Ser Asp Val Ile Met Glu Val Leu Asp Ala Arg Asp145 150 155 160Pro Leu Gly Thr Arg Cys Ile Asp Met Glu Lys Met Val Arg Lys Ala 165 170 175Asp Pro Ser Lys Arg Ile Val Leu Leu Leu Asn Lys Ile Asp Leu Val 180 185 190Pro Lys Glu Ala Ala Glu Lys Trp Leu Thr Tyr Leu Arg Glu Glu Leu 195 200 205Pro Thr Val Ala Phe Lys Cys Asn Thr Gln Glu Gln Arg Thr Lys Leu 210 215 220Gly Trp Lys Ser Ser Lys Leu Asp Lys Thr Ser Asn Ile Pro Gln Ser225 230 235 240Ser Asp Cys Leu Gly Ala Glu Asn Leu Ile Lys Leu Leu Lys Asn Tyr 245 250 255Ser Arg Ser His Glu Leu Lys Leu Thr Ile Thr Val Gly Ile Val Gly 260 265 270Leu Pro Asn Val Gly Lys Ser Ser Leu Ile Asn Ser Leu Lys Arg Ser 275 280 285Arg Val Val Asn Val Gly Ser Thr Pro Gly Ile Thr Arg Ser Met Gln 290 295 300Glu Val Gln Leu Asp Lys Lys Val Lys Leu Leu Asp Cys Pro Gly Val305 310 315 320Val Met Leu Lys Ser Ser Asn Ser Gly Val Ser Val Ala Leu Arg Asn 325 330 335Cys Lys Lys Val Glu Lys Ile Glu Asp Pro Val Ala Pro Val Lys Gln 340 345 350Ile Leu Ser Ile Cys Pro His Glu Lys Leu Leu Ser Leu Tyr Lys Val 355 360 365Pro Asn Phe Gly Ser Val Asp Asp Phe Leu Gln Lys Val Ala Thr Val 370 375 380Arg Gly Lys Leu Lys Lys Gly Gly Val Val Asp Val Glu Ala Ala Ala385 390 395 400Arg Ile Val Leu His Asp Trp Asn Glu Gly Lys Ile Pro Tyr Phe Thr 405 410 415Leu Pro Pro Lys Arg Asp Ala Gly Glu Asp Ser Asp Ala Val Ile Ile 420 425 430Ser Glu Asp Gly Lys Glu Phe Asn Ile Asp Glu Ile Tyr Lys Ala Glu 435 440 445Ser Ser Tyr Ile Gly Gly Leu Lys Ser Ile Glu Glu Phe His His Ile 450 455 460Glu Ile Pro Pro Asn Ala Pro Leu Ala Ile Asp Glu Glu Met Leu Glu465 470 475 480Asp Gly Gly Lys Lys Pro Ser Glu Ala Ile Gln Glu Ser Arg Asp Arg 485 490 495Glu Glu Gln Met Pro Asp Val Lys Asp Ser Gly Gly Ser Lys Ala Ala 500 505 510Ser Ala Ser Thr Gln Asn Asp Lys Leu Tyr Thr Ala Glu Gly Val Leu 515 520 525Asp Pro Arg Lys Ser Lys Ala Glu Lys Lys Arg Arg Lys Ala Ser Arg 530 535 540Pro Ser Ala Leu Asn Asp Met Asp Ala Asp Tyr Asp Phe Lys Val Asp545 550 555 560Tyr Arg Met Glu Asp Gly Gly Ser Glu Gly Ala His Ala Asp Asp Glu 565 570 575Asp Gly Gly Asp Gly Ser Glu Asp Asn Glu Pro Met Thr Gly Val Asp 580 585 590Asp Ala59423PRTZea mays 59Met Ala Gln Ala Val Val Pro Ala Met Gln Cys Arg Val Gly Val Lys1 5 10 15Ala Ala Ala Gly Arg Val Trp Ser Ala Gly Arg Thr Arg Thr Gly Arg 20 25 30Gly Gly Ala Ser Pro Gly Phe Lys Val Met Ala Val Ser Thr Gly Ser 35 40 45Thr Gly Val Val Pro Arg Leu Glu Gln Leu Leu Asn Met Asp Thr Thr 50 55 60Pro Tyr Thr Asp Lys Val Ile Ala Glu Tyr Ile Trp Val Gly Gly Ser65 70 75 80Gly Ile Asp Ile Arg Ser Lys Ser Arg Thr Ile Ser Lys Pro Val Glu 85 90 95Asp Pro Ser Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly 100 105 110Gln Ala Pro Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile 115 120 125Phe Lys Asp Pro Phe Arg Gly Gly Asn Asn Val Leu Val Ile Cys Asp 130 135 140Thr Tyr Thr Pro Gln Gly Glu Pro Leu Pro Thr Asn Lys Arg His Arg145 150 155 160Ala Ala Gln Ile Phe Ser Asp Pro Lys Val Ala Glu Gln Val Pro Trp 165 170 175Phe Gly Ile Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Asn Trp 180 185 190Pro Leu Gly Trp Pro Val Gly Gly Phe Pro Gly Pro Gln Gly Pro Tyr 195 200 205Tyr Cys Ala Val Gly Ala Asp Lys Ser Phe Gly Arg Asp Ile Ser Asp 210 215 220Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Thr225 230 235 240Asn Gly Glu Val Met Pro Gly Gln Trp Glu Tyr Gln Val Gly Pro Ser 245 250 255Val Gly Ile Glu Ala Gly Asp His Ile Trp Ile Ser Arg Tyr Ile Leu 260 265 270Glu Arg Ile Thr Glu Gln Ala Gly Val Val Leu Thr Leu Asp Pro Lys 275 280 285Pro Ile Gln Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser 290 295 300Thr Lys Thr Met Arg Glu Asp Gly Gly Phe Glu Glu Ile Lys Arg Ala305 310 315 320Ile Leu Asn Leu Ser Leu Arg His Asp Leu His Ile Ser Ala Tyr Gly 325 330 335Glu Gly Asn Glu Arg Arg Leu Thr Gly Lys His Glu Thr Ala Ser Ile 340 345 350Gly Thr Phe Ser Trp Gly Val Ala Asn Arg Gly Cys Ser Ile Arg Val 355 360 365Gly Arg Asp Thr Glu Ala Lys Gly Lys Gly Tyr Leu Glu Asp Arg Arg 370 375 380Pro Ala Ser Asn Met Asp Pro Tyr Ile Val Thr Gly Leu Leu Ala Glu385 390 395 400Thr Thr Ile Leu Trp Gln Pro Ser Leu Glu Ala Glu Ala Leu Ala Ala 405 410 415Lys Lys Leu Ala Leu Lys Val 42060971PRTZea mays 60Met Ile Val Gln Pro Ile Glu Leu Arg Ala Trp Thr Ala Phe Pro Gly1 5 10 15Ser Ala Gln Glu Gly Ile Gly Arg Met Ala Ala Ser Val Ser Arg Ala 20 25 30Ile Cys Val Gln Lys Pro Gly Ser Lys Cys Thr Arg Asp Arg Glu Ala 35 40 45Thr Ser Phe Ala Arg Arg Ser Val Ala Ala Pro Arg Pro Pro His Ala 50 55 60Lys Ala Ala Gly Val Ile Arg Ser Asp Ser Gly Ala Gly Arg Gly Gln65 70 75 80His Cys Ser Pro Leu Arg Ala Val Val Asp Ala Ala Pro Ile Gln Thr 85 90 95Thr Lys Lys Arg Val Phe His Phe Gly Lys Gly Lys Ser Glu Gly Asn 100 105 110Lys Thr Met Lys Glu Leu Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu 115 120 125Met Ala Ser Ile Gly Leu Ser Val Pro Pro Gly Phe Thr Val Ser Thr 130 135 140Glu Ala Cys Gln Gln Tyr Gln Asp Ala Gly Cys Ala Leu Pro Ala Gly145 150 155 160Leu Trp Ala Glu Ile Val Asp Gly Leu Gln Trp Val Glu Glu Tyr Met 165 170 175Gly Ala Thr Leu Gly Asp Pro Gln Arg Pro Leu Leu Leu Ser Val Arg 180 185 190Ser Gly Ala Ala Val Ser Met Pro Gly Met Met Asp Thr Val Leu Asn 195 200 205Leu Gly Leu Asn Asp Glu Val Ala Ala Gly Leu Ala Ala Lys Ser Gly 210 215 220Glu Arg Phe Ala Tyr Asp Ser Phe Arg Arg Phe Leu Asp Met Phe Gly225 230 235 240Asn Val Val Met Asp Ile Pro Arg Ser Leu Phe Glu Glu Lys Leu Glu 245 250 255His Met Lys Glu Ser Lys Gly Leu Lys Asn Asp Thr Asp Leu Thr Ala 260 265 270Ser Asp Leu Lys Glu Leu Val Gly Gln Tyr Lys Glu Val Tyr Leu Ser 275 280 285Ala Lys Gly Glu Pro Phe Pro Ser Asp Pro Lys Lys Gln Leu Glu Leu 290 295 300Ala Val Leu Ala Val Phe Asn Ser Trp Glu Ser Pro Arg Ala Lys Lys305 310 315 320Tyr Arg Ser Ile Asn Gln Ile Thr Gly Leu Arg Gly Thr Ala Val Ser 325 330 335Val Gln Cys Met Val Phe Gly Asn Met Gly Asn Thr Ser Asp Thr Gly 340 345 350Val Leu Phe Thr Arg Asn Pro Asn Thr Gly Glu Lys Lys Leu Tyr Gly 355 360 365Glu Phe Leu Val Asn Ala Gln Gly Glu Asp Val Val Ala Gly Ile Arg 370 375 380Thr Pro Glu Asp Leu Asp Ala Met Lys Asn Leu Met Pro Gln Ala Tyr385 390 395 400Asp Glu Leu Val Glu Asn Cys Asn Ile Leu Glu Ser His Tyr Lys Glu 405 410 415Met Gln Asp Ile Glu Phe Thr Val Gln Glu Asn Arg Leu Trp Met Leu 420 425 430Gln Cys Arg Thr Gly Lys Arg Thr Gly Lys Ser Ala Val Lys Ile Ala 435 440 445Val Asp Met Val Asn Glu Gly Leu Val Glu Pro Arg Ser Ala Ile Lys 450 455 460Met Val Glu Pro Gly His Leu Asp Gln Leu Leu His Pro Gln Phe Glu465 470 475 480Asn Pro Ser Thr Tyr Lys Asp Gln Val Ile Ala Thr Gly Leu Pro Ala 485 490 495Ser Pro Gly Ala Ala Val Gly Gln Val Val Phe Thr Ala Glu Asp Ala 500 505 510Glu Ala Trp His Pro Gln Gly Lys Ala Ala Ile Leu Val Arg Ala Glu 515 520 525Thr Ser Pro Glu Asp Val Gly Gly Met His Ala Ala Val Gly Ile Leu 530 535 540Thr Glu Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg Gly545 550 555 560Trp Gly Lys Cys Cys Val Ser Gly Cys Ser Gly Ile Arg Val Asn Asp 565 570 575Ala Glu Lys Leu Val Thr Ile Gly Gly His Val Leu Arg Glu Gly Glu 580 585 590Trp Leu Ser Leu Asn Gly Ser Ala Gly Glu Val Ile Leu Gly Lys Gln 595 600 605Pro Leu Ser Pro Pro Ala Leu Ser Gly Asp Leu Gly Thr Phe Met Ala 610 615 620Trp Val Asp Asp Val Arg Lys Leu Lys Val Leu Ala Asn Ala Asp Thr625 630 635 640Pro Asp Asp Ala Leu Thr Ala Arg Asn Asn Gly Ala Gln Gly Ile Gly 645 650 655Leu Cys Arg Thr Glu His Met Phe Phe Ala Ser Asp Glu Arg Ile Lys 660 665 670Ala Val Arg Gln Met Ile Met Ala Pro Thr Leu Glu Leu Arg Gln Gln 675 680 685Ala Leu Asp Arg Leu Leu Pro Tyr Gln Arg Ser Asp Phe Glu Gly Ile 690 695 700Phe Arg Ala Met Asp Gly Leu Pro Val Thr Ile Arg Leu Leu Asp Pro705 710 715 720Pro Leu His Glu Phe Leu Pro Glu Gly Asn Ile Glu Asp Ile Val Ser 725 730 735Glu Leu Cys Ala Glu Thr Gly Ala Asn Gln Glu Asp Ala Leu Ala Arg 740 745 750Ile Glu Lys Leu Ser Glu Val Asn Pro Met Leu Gly Phe Arg Gly Cys 755 760 765Arg Leu Gly Ile Ser Tyr Pro Glu Leu Thr Glu Met Gln Ala Arg Ala 770 775 780Ile Phe Glu Ala Ala Ile Ala Met Thr Asn Gln Gly Val Gln Val Phe785 790 795 800Pro Glu Ile Met Val Pro Leu Val Gly Thr Pro Gln Glu Leu Gly His 805 810 815Gln Val Thr Leu Ile Arg Gln Val Ala Glu Lys Val Phe Ala Asn Val 820 825 830Gly Lys Thr Ile Gly Tyr Lys Val Gly Thr Met Ile Glu Ile Pro Arg 835 840 845Ala Ala Leu Val Ala Asp Glu Ile Ala Glu Gln Ala Glu Phe Phe Ser 850 855 860Phe Gly Thr Asn Asp Leu Thr Gln Met Thr Phe Gly Tyr Ser Arg Asp865 870 875 880Asp Val Gly Lys Phe Ile Pro Val Tyr Leu Ala Gln Gly Ile Leu Lys 885 890 895His Asp Pro Phe Glu Val Leu Asp Gln Arg Gly Val Gly Glu Leu Val 900 905 910Lys Phe Ala Thr Glu Arg Gly Arg Lys Ala Arg Pro Asn Leu Lys Val 915 920 925Gly Ile Cys Gly Glu His Gly Gly Glu Pro Ser Ser Val Ala Phe Phe 930 935 940Ala Lys Ala Gly Leu Asp Tyr Val Ser Cys Ser Pro Phe Arg Val Pro945 950 955 960Ile Ala Arg Leu Ala Ala Ala Gln Val Leu Val 965 97061263PRTSorghum bicolor 61Met Leu Glu Leu Arg Leu Val Gln Gly Ser Leu Leu Lys Lys Val Leu1 5 10 15Glu Ala Ile Arg Glu Leu Val Thr Asp Ala Asn Phe Asp Cys Ser Gly 20 25 30Thr Gly Phe Ser Leu Gln Ala Met Asp Ser Ser His Val Ala Leu Val 35 40 45Ala Leu Leu Leu Arg Ala Glu Gly

Phe Glu His Tyr Arg Cys Asp Arg 50 55 60Asn Leu Ser Met Gly Met Asn Leu Asn Asn Met Ala Lys Met Leu Arg65 70 75 80Cys Ala Gly Asn Asp Asp Ile Ile Thr Ile Lys Ala Asp Asp Gly Ser 85 90 95Asp Thr Val Thr Phe Met Phe Glu Ser Pro Lys Gln Asp Lys Ile Ala 100 105 110Asp Phe Glu Met Lys Leu Met Asp Ile Asp Ser Glu His Leu Gly Ile 115 120 125Pro Asp Ser Glu Tyr Gln Ala Ile Val Arg Met Pro Ser Ala Glu Phe 130 135 140Met Arg Ile Cys Lys Asp Leu Ser Ser Ile Gly Asp Thr Val Val Ile145 150 155 160Ser Val Thr Lys Glu Gly Val Lys Phe Ser Thr Ser Gly Glu Ile Gly 165 170 175Ser Ala Asn Ile Val Cys Arg Gln Asn Gln Thr Ile Asp Lys Pro Glu 180 185 190Glu Ala Thr Ile Ile Glu Met Gln Glu Pro Val Ser Leu Thr Phe Ala 195 200 205Leu Arg Tyr Met Asn Ser Phe Thr Lys Ala Ser Ser Leu Ser Glu Gln 210 215 220Val Thr Ile Ser Leu Ser Ser Glu Leu Pro Val Val Val Glu Tyr Lys225 230 235 240Ile Ala Glu Met Gly Tyr Ile Arg Phe Tyr Leu Ala Pro Lys Ile Glu 245 250 255Glu Asp Glu Glu Met Lys Ala 26062447PRTEscherichia coli CFT073 62Met Asp Gln Thr Tyr Ser Leu Glu Ser Phe Leu Asn His Val Gln Lys1 5 10 15Arg Asp Pro Asn Gln Thr Glu Phe Ala Gln Ala Val Arg Glu Val Met 20 25 30Thr Thr Leu Trp Pro Phe Leu Glu Gln Asn Pro Lys Tyr Arg Gln Met 35 40 45Ser Leu Leu Glu Arg Leu Val Glu Pro Glu Arg Val Ile Gln Phe Arg 50 55 60Val Val Trp Val Asp Asp Arg Asn Gln Val Gln Val Asn Arg Ala Trp65 70 75 80Arg Val Gln Phe Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Met Arg 85 90 95Phe His Pro Ser Val Asn Leu Ser Ile Leu Lys Phe Leu Gly Phe Glu 100 105 110Gln Thr Phe Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 115 120 125Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Gly Glu Val Met 130 135 140Arg Phe Cys Gln Ala Leu Met Thr Glu Leu Tyr Arg His Leu Gly Ala145 150 155 160Asp Thr Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Val 165 170 175Gly Phe Met Ala Gly Met Met Lys Lys Leu Ser Asn Asn Thr Ala Cys 180 185 190Val Phe Thr Gly Lys Gly Leu Ser Phe Gly Gly Ser Leu Ile Arg Pro 195 200 205Glu Ala Thr Gly Tyr Gly Leu Val Tyr Phe Thr Glu Ala Met Leu Lys 210 215 220Arg His Gly Met Gly Phe Glu Gly Met Arg Val Ser Val Ser Gly Ser225 230 235 240Gly Asn Val Ala Gln Tyr Ala Ile Glu Lys Ala Met Glu Phe Gly Ala 245 250 255Arg Val Ile Thr Ala Ser Asp Ser Ser Gly Thr Val Val Asp Glu Ser 260 265 270Gly Phe Thr Lys Glu Lys Leu Ala Arg Leu Ile Glu Ile Lys Ala Ser 275 280 285Arg Asp Gly Arg Val Ala Asp Tyr Ala Lys Glu Phe Gly Leu Val Tyr 290 295 300Leu Glu Gly Gln Gln Pro Trp Ser Val Pro Val Asp Ile Ala Leu Pro305 310 315 320Cys Ala Thr Gln Asn Glu Leu Asp Val Asp Ala Ala His Gln Leu Ile 325 330 335Ala Asn Gly Val Lys Ala Val Ala Glu Gly Ala Asn Met Pro Thr Thr 340 345 350Ile Glu Ala Thr Glu Leu Phe Gln Gln Ala Gly Val Leu Phe Ala Pro 355 360 365Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met 370 375 380Ala Gln Asn Ala Ala Arg Leu Gly Trp Lys Ala Glu Lys Val Asp Ala385 390 395 400Arg Leu His His Ile Met Leu Asp Ile His His Ala Cys Val Glu His 405 410 415Gly Gly Glu Gly Glu Gln Thr Asn Tyr Val Gln Gly Ala Asn Ile Ala 420 425 430Gly Phe Val Lys Val Ala Asp Ala Met Leu Ser Gln Gly Val Ile 435 440 44563594PRTZea mays 63Met Val Lys Arg Ser Lys Lys Ser Lys Ser Lys Arg Val Thr Leu Arg1 5 10 15Gln Lys His Lys Val Gln Arg Lys Val Lys Glu His His Arg Lys Lys 20 25 30Arg Lys Glu Ala Lys Lys Ala Gly Lys Ala Gly Gln Arg Arg Lys Val 35 40 45Glu Lys Asp Pro Gly Ile Pro Asn Glu Trp Pro Phe Lys Glu Gln Glu 50 55 60Leu Lys Ala Leu Glu Ala Arg Arg Ala Gln Ala Leu Gln Glu Leu Glu65 70 75 80Leu Lys Lys Gln Ala Arg Lys Glu Arg Ala Gln Lys Arg Lys Ala Gly 85 90 95Leu Leu Glu Asp Glu Asp Ile Ala Ser Leu Ala Ser Ala Ala Ser Ala 100 105 110Gln Gly Ser Glu Phe Ala Ala Lys Glu Asn Ala Pro Leu Leu Val Ala 115 120 125Lys Ile Asn Asp His Ser Glu Arg Ser Phe Tyr Lys Glu Leu Val Lys 130 135 140Val Ile Glu Ala Ser Asp Val Ile Val Glu Val Leu Asp Ala Arg Asp145 150 155 160Pro Leu Gly Thr Arg Cys Ile Asp Met Glu Lys Met Val Arg Lys Ala 165 170 175Asp Pro Ser Lys Arg Ile Val Leu Leu Leu Asn Lys Ile Asp Leu Val 180 185 190Pro Lys Glu Ala Ala Glu Lys Trp Leu Thr Tyr Leu Arg Glu Glu Leu 195 200 205Pro Thr Val Ala Phe Lys Cys Asn Thr Gln Glu Gln Arg Thr Lys Leu 210 215 220Gly Trp Lys Ser Ser Lys Leu Asp Lys Thr Ser Asn Ile Pro Gln Ser225 230 235 240Ser Asp Cys Leu Gly Ala Glu Asn Leu Ile Lys Leu Leu Lys Asn Tyr 245 250 255Ser Arg Ser His Glu Leu Lys Leu Ala Ile Thr Val Gly Ile Val Gly 260 265 270Leu Pro Asn Val Gly Lys Ser Ser Leu Ile Asn Ser Leu Lys Arg Ser 275 280 285Arg Val Val Asn Val Gly Ser Thr Pro Gly Ile Thr Arg Ser Met Gln 290 295 300Glu Val Gln Leu Asp Lys Lys Val Lys Leu Leu Asp Cys Pro Gly Val305 310 315 320Val Met Leu Lys Ser Ser Asn Ser Gly Val Ser Val Ala Leu Arg Asn 325 330 335Cys Lys Lys Val Glu Lys Ile Glu Asp Pro Val Ala Pro Val Lys Glu 340 345 350Ile Leu Ser Ile Cys Pro His Glu Lys Leu Leu Ser Leu Tyr Lys Val 355 360 365Pro Asn Phe Gly Ser Val Asp Asp Phe Leu Gln Lys Val Ala Thr Val 370 375 380Arg Gly Lys Leu Lys Lys Gly Gly Val Val Asp Val Glu Ala Ala Ala385 390 395 400Arg Ile Val Leu His Asp Trp Asn Glu Gly Lys Ile Pro Tyr Phe Thr 405 410 415Leu Pro Pro Lys Arg Asp Ala Gly Glu Asp Ser Asp Ala Val Ile Ile 420 425 430Ser Glu Asp Gly Lys Glu Phe Asn Ile Asp Asp Ile Tyr Lys Ala Glu 435 440 445Ser Ser Tyr Ile Gly Gly Leu Lys Ser Ile Glu Glu Phe His His Ile 450 455 460Glu Ile Pro Pro Asn Ala Pro Pro Ala Ile Asp Glu Glu Met Leu Glu465 470 475 480Asp Gly Gly Lys Lys Pro Ser Glu Ala Ile Gln Glu Ser Arg Asp Arg 485 490 495Glu Glu Gln Met Pro Asp Val Lys Asp Ser Gly Gly Ser Lys Ala Ala 500 505 510Ser Ala Ser Thr Gln Asn Asp Lys Leu Tyr Thr Ala Glu Gly Val Leu 515 520 525Asp Pro Arg Lys Ser Lys Ala Glu Lys Lys Arg Arg Lys Ala Ser Arg 530 535 540Pro Ser Ala Leu Asn Asp Met Asp Ala Asp Tyr Asp Phe Lys Val Asp545 550 555 560Tyr Arg Met Glu Asp Gly Gly Ser Glu Gly Ala His Ala Asp Asp Glu 565 570 575Asp Gly Gly Asp Gly Ser Glu Asp Asn Glu Pro Met Thr Gly Val Asp 580 585 590Asp Ala64178PRTZea mays 64Met Ala Glu Ala Pro Ala Ser Pro Gly Gly Gly Gly Gly Ser His Glu1 5 10 15Ser Gly Ser Pro Arg Gly Gly Gly Gly Gly Gly Ser Val Arg Glu Gln 20 25 30Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala 35 40 45Ile Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln 50 55 60Glu Cys Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala Ser Asp65 70 75 80Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu 85 90 95Trp Ala Met Ala Thr Leu Gly Phe Glu Asp Tyr Ile Glu Pro Leu Lys 100 105 110Val Tyr Leu Gln Lys Tyr Arg Glu Met Glu Gly Asp Ser Lys Leu Thr 115 120 125Ala Lys Ser Ser Asp Gly Ser Ile Lys Lys Asp Ala Leu Gly His Val 130 135 140Gly Ala Ser Ser Ser Ala Ala Glu Gly Met Gly Gln Gln Gly Ala Tyr145 150 155 160Asn Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His Asn Gly Asp Ile 165 170 175Ser Asn65447PRTEscherichia coli O157H7 EDL933 65Met Asp Gln Thr Tyr Ser Leu Glu Ser Phe Leu Asn His Val Gln Lys1 5 10 15Arg Asp Pro Asn Gln Thr Glu Phe Ala Gln Ala Val Arg Glu Val Met 20 25 30Thr Thr Leu Trp Pro Phe Leu Glu Gln Asn Pro Lys Tyr Arg Gln Met 35 40 45Ser Leu Leu Glu Arg Leu Val Glu Pro Glu Arg Val Ile Gln Phe Arg 50 55 60Val Val Trp Val Asp Asp Arg Asn Gln Val Gln Val Asn Arg Ala Trp65 70 75 80Arg Val Gln Phe Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Met Arg 85 90 95Phe His Pro Ser Val Asn Leu Ser Ile Leu Lys Phe Leu Gly Phe Glu 100 105 110Gln Thr Phe Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 115 120 125Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Gly Glu Val Met 130 135 140Arg Phe Cys Gln Ala Leu Met Thr Glu Leu Tyr Arg His Leu Gly Ala145 150 155 160Asp Thr Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Val 165 170 175Gly Phe Met Ala Gly Met Met Lys Lys Leu Ser Asn Asn Thr Ala Cys 180 185 190Val Phe Thr Gly Lys Gly Leu Ser Phe Gly Gly Ser Leu Ile Arg Pro 195 200 205Glu Ala Thr Gly Tyr Gly Leu Val Tyr Phe Thr Glu Ala Met Leu Lys 210 215 220Arg His Gly Met Gly Phe Glu Gly Met Arg Val Ser Val Ser Gly Ser225 230 235 240Gly Asn Val Ala Gln Tyr Ala Ile Glu Lys Ala Met Glu Phe Gly Ala 245 250 255Arg Val Ile Thr Ala Ser Asp Ser Ser Gly Thr Val Val Asp Glu Ser 260 265 270Gly Phe Thr Lys Glu Lys Leu Ala Arg Leu Ile Glu Ile Lys Ser Ser 275 280 285Arg Asp Gly Arg Val Ala Asp Tyr Ala Lys Glu Phe Gly Leu Val Tyr 290 295 300Leu Glu Gly Gln Gln Pro Trp Ser Val Pro Val Asp Ile Ala Leu Pro305 310 315 320Cys Ala Thr Gln Asn Glu Leu Asp Val Asp Ala Ala His Gln Leu Ile 325 330 335Ala Asn Gly Val Lys Ala Val Ala Glu Gly Ala Asn Met Pro Thr Thr 340 345 350Ile Glu Ala Thr Glu Leu Phe Gln Gln Ala Gly Val Leu Phe Ala Pro 355 360 365Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met 370 375 380Ala Gln Asn Ala Ala Arg Leu Gly Trp Lys Ala Glu Lys Val Asp Ala385 390 395 400Arg Leu His His Ile Met Leu Asp Ile His His Ala Cys Val Glu His 405 410 415Gly Gly Glu Gly Glu Gln Thr Asn Tyr Val Gln Gly Ala Asn Ile Ala 420 425 430Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 435 440 44566447PRTEscherichia coli 66Met Asp Gln Thr Tyr Ser Leu Glu Ser Phe Leu Asn His Val Gln Lys1 5 10 15Arg Asp Pro Asn Gln Thr Glu Phe Ala Gln Ala Val Arg Glu Val Met 20 25 30Thr Thr Leu Trp Pro Phe Leu Glu Gln Asn Pro Lys Tyr Arg Gln Met 35 40 45Ser Leu Leu Glu Arg Leu Val Glu Pro Glu Arg Val Ile Gln Phe Arg 50 55 60Val Val Trp Val Asp Asp Arg Asn Gln Ile Gln Val Asn Arg Ala Trp65 70 75 80Arg Val Gln Phe Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Met Arg 85 90 95Phe His Pro Ser Val Asn Leu Ser Ile Leu Lys Phe Leu Gly Phe Glu 100 105 110Gln Thr Phe Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 115 120 125Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Gly Glu Val Met 130 135 140Arg Phe Cys Gln Ala Leu Met Thr Glu Leu Tyr Arg His Leu Gly Ala145 150 155 160Asp Thr Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Val 165 170 175Gly Phe Met Ala Gly Met Met Lys Lys Leu Ser Asn Asn Thr Ala Cys 180 185 190Val Phe Thr Gly Lys Gly Leu Ser Phe Gly Gly Ser Leu Ile Arg Pro 195 200 205Glu Ala Thr Gly Tyr Gly Leu Val Tyr Phe Thr Glu Ala Met Leu Lys 210 215 220Arg His Gly Met Gly Phe Glu Gly Met Arg Val Ser Val Ser Gly Ser225 230 235 240Gly Asn Val Ala Gln Tyr Ala Ile Glu Lys Ala Met Glu Phe Gly Ala 245 250 255Arg Val Ile Thr Ala Ser Asp Ser Ser Gly Thr Val Val Asp Glu Ser 260 265 270Gly Phe Thr Lys Glu Lys Leu Ala Arg Leu Ile Glu Ile Lys Ala Ser 275 280 285Arg Asp Gly Arg Val Ala Asp Tyr Ala Lys Glu Phe Gly Leu Val Tyr 290 295 300Leu Glu Gly Gln Gln Pro Trp Ser Leu Pro Val Asp Ile Ala Leu Pro305 310 315 320Cys Ala Thr Gln Asn Glu Leu Asp Val Asp Ala Ala His Gln Leu Ile 325 330 335Ala Asn Gly Val Lys Ala Val Ala Glu Gly Ala Asn Met Pro Thr Thr 340 345 350Ile Glu Ala Thr Glu Leu Phe Gln Gln Ala Gly Val Leu Phe Ala Pro 355 360 365Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met 370 375 380Pro Gln Asn Ala Ala Arg Leu Gly Trp Lys Ala Glu Lys Val Asp Ala385 390 395 400Arg Leu His His Ile Met Leu Asp Ile His His Ala Cys Val Glu His 405 410 415Gly Gly Glu Gly Glu Gln Thr Asn Tyr Val Gln Gly Ala Asn Ile Ala 420 425 430Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 435 440 44567947PRTZea mays 67Met Ala Ala Ser Val Ser Arg Ala Ile Cys Val Gln Lys Pro Gly Ser1 5 10 15Lys Cys Thr Arg Asp Arg Glu Ala Thr Ser Phe Ala Arg Arg Ser Val 20 25 30Ala Ala Pro Arg Pro Pro His Ala Lys Ala Ala Gly Val Ile Arg Ser 35 40 45Asp Ser Gly Ala Gly Arg Gly Gln His Cys Ser Pro Leu Arg Ala Val 50 55 60Val Asp Ala Ala Pro Ile Gln Thr Thr Lys Lys Arg Val Phe His Phe65 70 75 80Gly Lys Gly Lys Ser Glu Gly Asn Lys Thr Met Lys Glu Leu Leu Gly 85 90 95Gly Lys Gly Ala Asn Leu Ala Glu Met Ala Ser Ile Gly Leu Ser Val 100 105 110Pro Pro Gly Phe Thr Val Ser Thr Glu Ala Cys Gln Gln Tyr Gln Asp 115 120 125Ala Gly Cys Ala Leu Pro Ala Gly Leu Trp Ala

Glu Ile Val Asp Gly 130 135 140Leu Gln Trp Val Glu Glu Tyr Met Gly Ala Thr Leu Gly Asp Pro Gln145 150 155 160Arg Pro Leu Leu Leu Ser Val Arg Ser Gly Ala Ala Val Ser Met Pro 165 170 175Gly Met Met Asp Thr Val Leu Asn Leu Gly Leu Asn Asp Glu Val Ala 180 185 190Ala Gly Leu Ala Ala Lys Ser Gly Glu Arg Phe Ala Tyr Asp Ser Phe 195 200 205Arg Arg Phe Leu Asp Met Phe Gly Asn Val Val Met Asp Ile Pro Arg 210 215 220Ser Leu Phe Glu Glu Lys Leu Glu His Met Lys Glu Ser Lys Gly Leu225 230 235 240Lys Asn Asp Thr Asp Leu Thr Ala Ser Asp Leu Lys Glu Leu Val Gly 245 250 255Gln Tyr Lys Glu Val Tyr Leu Ser Ala Lys Gly Glu Pro Phe Pro Ser 260 265 270Asp Pro Lys Lys Gln Leu Glu Leu Ala Val Leu Ala Val Phe Asn Ser 275 280 285Trp Glu Ser Pro Arg Ala Lys Lys Tyr Arg Ser Ile Asn Gln Ile Thr 290 295 300Gly Leu Arg Gly Thr Ala Val Asn Val Gln Cys Met Val Phe Gly Asn305 310 315 320Met Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro Asn 325 330 335Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln Gly 340 345 350Glu Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp Ala Met 355 360 365Lys Asn Leu Met Pro Gln Ala Tyr Asp Glu Leu Val Glu Asn Cys Asn 370 375 380Ile Leu Glu Ser His Tyr Lys Glu Met Gln Asp Ile Glu Phe Thr Val385 390 395 400Gln Glu Asn Arg Leu Trp Met Leu Gln Cys Arg Thr Gly Lys Arg Thr 405 410 415Gly Lys Ser Ala Val Lys Ile Ala Val Asp Met Val Asn Glu Gly Leu 420 425 430Val Glu Pro Arg Ser Ala Ile Lys Met Val Glu Pro Gly His Leu Asp 435 440 445Gln Leu Leu His Pro Gln Phe Glu Asn Pro Ser Ala Tyr Lys Asp Gln 450 455 460Val Ile Ala Thr Gly Leu Pro Ala Ser Pro Gly Ala Ala Val Gly Gln465 470 475 480Val Val Phe Thr Ala Glu Asp Ala Glu Ala Trp His Ser Gln Gly Lys 485 490 495Ala Ala Ile Leu Val Arg Ala Glu Thr Ser Pro Glu Asp Val Gly Gly 500 505 510Met His Ala Ala Val Gly Ile Leu Thr Glu Arg Gly Gly Met Thr Ser 515 520 525His Ala Ala Val Val Ala Arg Trp Trp Gly Lys Cys Cys Val Ser Gly 530 535 540Cys Ser Gly Ile Arg Val Asn Asp Ala Glu Lys Leu Val Thr Ile Gly545 550 555 560Ser His Val Leu Arg Glu Gly Glu Trp Leu Ser Leu Asn Gly Ser Thr 565 570 575Gly Glu Val Ile Leu Gly Lys Gln Pro Leu Ser Pro Pro Ala Leu Ser 580 585 590Gly Asp Leu Gly Thr Phe Met Ala Trp Val Asp Asp Val Arg Lys Leu 595 600 605Lys Val Leu Ala Asn Ala Asp Thr Pro Asp Asp Ala Leu Thr Ala Arg 610 615 620Asn Asn Gly Ala Gln Gly Ile Gly Leu Cys Arg Thr Glu His Met Phe625 630 635 640Phe Ala Ser Asp Glu Arg Ile Lys Ala Val Arg Gln Met Ile Met Ala 645 650 655Pro Thr Leu Glu Leu Arg Gln Gln Ala Leu Asp Arg Leu Leu Thr Tyr 660 665 670Gln Arg Ser Asp Phe Glu Gly Ile Phe Arg Ala Met Asp Gly Leu Pro 675 680 685Val Thr Ile Arg Leu Leu Asp His Pro Ser Tyr Glu Phe Leu Pro Glu 690 695 700Gly Asn Ile Glu Asp Ile Val Ser Glu Leu Cys Ala Glu Thr Gly Ala705 710 715 720Asn Gln Glu Asp Ala Leu Ala Arg Ile Glu Lys Leu Ser Glu Val Asn 725 730 735Pro Met Leu Gly Phe Arg Gly Cys Arg Leu Gly Ile Ser Tyr Pro Glu 740 745 750Leu Thr Glu Met Gln Ala Arg Ala Ile Phe Glu Ala Ala Ile Ala Met 755 760 765Thr Asn Gln Gly Val Gln Val Phe Pro Glu Ile Met Val Pro Leu Val 770 775 780Gly Thr Pro Gln Glu Leu Gly His Gln Val Thr Leu Ile Arg Gln Val785 790 795 800Ala Glu Lys Val Phe Ala Asn Val Gly Lys Thr Ile Gly Tyr Lys Val 805 810 815Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Leu Val Ala Asp Glu Ile 820 825 830Ala Glu Gln Ala Glu Phe Phe Ser Phe Gly Thr Asn Asp Leu Thr Gln 835 840 845Met Thr Phe Gly Tyr Ser Arg Asp Asp Val Gly Lys Phe Ile Pro Val 850 855 860His Leu Ala Gln Gly Ile Leu Gln His Asp Pro Phe Glu Val Leu Asp865 870 875 880Gln Arg Gly Val Gly Glu Leu Val Lys Phe Ala Thr Glu Arg Gly Arg 885 890 895Lys Ala Arg Pro Asn Leu Lys Val Gly Ile Cys Gly Glu His Gly Gly 900 905 910Glu Pro Ser Ser Val Ala Phe Phe Ala Lys Ala Gly Leu Asp Phe Val 915 920 925Ser Cys Ser Pro Phe Arg Val Pro Ile Ala Arg Leu Ala Ala Ala Gln 930 935 940Val Leu Val94568179PRTZea mays 68Met Ala Glu Ala Pro Ala Ser Pro Gly Gly Gly Gly Gly Ser His Glu1 5 10 15Ser Gly Ser Pro Arg Gly Gly Gly Gly Gly Gly Ser Val Arg Glu Gln 20 25 30Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala 35 40 45Ile Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln 50 55 60Glu Cys Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala Ser Asp65 70 75 80Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu 85 90 95Trp Ala Met Ala Thr Leu Gly Phe Glu Asp Tyr Ile Glu Pro Leu Lys 100 105 110Val Tyr Leu Gln Lys Tyr Arg Glu Met Glu Gly Asp Ser Lys Leu Thr 115 120 125Ala Lys Ser Ser Asp Gly Ser Ile Lys Lys Asp Ala Leu Gly His Val 130 135 140Gly Ala Ser Ser Ser Ala Ala Glu Gly Met Gly Gln Gln Gly Ala Tyr145 150 155 160Asn Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His Asn Gly Asp Ile 165 170 175Ser Asn Val69447PRTEscherichia coli 042 69Met Asp Gln Thr Tyr Ser Leu Glu Ser Phe Leu Asn His Val Gln Lys1 5 10 15Arg Asp Pro Asn Gln Thr Glu Phe Ala Gln Ala Val Arg Glu Val Met 20 25 30Thr Thr Leu Trp Pro Phe Leu Glu Gln Asn Pro Lys Tyr Arg Gln Met 35 40 45Ser Leu Leu Glu Arg Leu Val Glu Pro Glu Arg Val Ile Gln Phe Arg 50 55 60Val Val Trp Val Asp Asp Arg Asn Gln Val Gln Val Asn Arg Ala Trp65 70 75 80Arg Val Gln Phe Ser Ser Ala Ile Gly Pro Tyr Lys Gly Gly Met Arg 85 90 95Phe His Pro Ser Val Asn Leu Ser Ile Leu Lys Phe Leu Gly Phe Glu 100 105 110Gln Thr Phe Lys Asn Ala Leu Thr Thr Leu Pro Met Gly Gly Gly Lys 115 120 125Gly Gly Ser Asp Phe Asp Pro Lys Gly Lys Ser Glu Gly Glu Val Met 130 135 140Arg Phe Cys Gln Ala Leu Met Thr Glu Leu Tyr Arg His Leu Gly Ala145 150 155 160Asp Thr Asp Val Pro Ala Gly Asp Ile Gly Val Gly Gly Arg Glu Val 165 170 175Gly Phe Met Ala Gly Met Met Lys Lys Leu Ser Asn Asn Thr Ala Cys 180 185 190Val Phe Thr Gly Lys Gly Leu Ser Phe Gly Gly Ser Leu Ile Arg Pro 195 200 205Glu Ala Thr Gly Tyr Gly Leu Val Tyr Phe Thr Glu Ala Met Leu Lys 210 215 220Arg His Gly Met Gly Phe Glu Gly Met Arg Val Ser Val Ser Gly Ser225 230 235 240Gly Asn Val Ala Gln Tyr Ala Ile Glu Lys Ala Met Glu Phe Gly Ala 245 250 255Arg Val Ile Thr Ala Ser Asp Ser Ser Gly Thr Val Val Asp Glu Ser 260 265 270Gly Phe Thr Lys Glu Lys Leu Ala Arg Leu Ile Glu Ile Lys Ala Ser 275 280 285Arg Asp Gly Arg Val Ala Asp Tyr Ala Lys Glu Phe Gly Leu Val Tyr 290 295 300Leu Glu Gly Gln Gln Pro Trp Ser Val Pro Val Asp Ile Ala Leu Pro305 310 315 320Cys Ala Thr Gln Asn Glu Leu Asp Val Asp Ala Ala His Gln Leu Ile 325 330 335Ala Thr Gly Val Lys Ala Val Ala Glu Gly Ala Asn Met Pro Thr Thr 340 345 350Ile Glu Ala Thr Glu Leu Phe Gln Gln Ala Gly Val Leu Phe Ala Pro 355 360 365Gly Lys Ala Ala Asn Ala Gly Gly Val Ala Thr Ser Gly Leu Glu Met 370 375 380Ala Gln Asn Ala Ala Arg Leu Gly Trp Lys Ala Glu Lys Val Asp Ala385 390 395 400Arg Leu His His Ile Met Leu Asp Ile His His Ala Cys Val Glu His 405 410 415Gly Gly Glu Gly Glu Gln Thr Asn Tyr Val Gln Gly Ala Asn Ile Ala 420 425 430Gly Phe Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Ile 435 440 44570345PRTGlycine max 70Met Cys Gly Gly Ala Ile Ile Ser Asp Phe Ile Pro Ala Ala Ala Ile1 5 10 15Ala Gly Ser Arg Arg Leu Thr Ala Asp Tyr Leu Trp Pro Asp Leu Lys 20 25 30Lys Arg Lys Ser Asp Leu Asp Val Asp Phe Glu Ala Asp Phe Arg Asp 35 40 45Phe Lys Asp Asp Ser Asp Ile Asp Asp Asp Asp Asp Asp His Gln Val 50 55 60Lys Pro Phe Ala Phe Ala Ala Ser Ser Arg Leu Ser Thr Ala Ala Lys65 70 75 80Ser Val Ala Phe Gln Gly Arg Ala Glu Ile Ser Ala Asn Arg Lys Arg 85 90 95Lys Asn Gln Tyr Arg Gly Ile Arg Gln Arg Pro Trp Gly Lys Trp Ala 100 105 110Ala Glu Ile Arg Asp Pro Arg Lys Gly Val Arg Val Trp Leu Gly Thr 115 120 125Phe Asn Thr Ala Glu Glu Ala Ala Arg Ala Tyr Asp Ala Glu Ala Arg 130 135 140Arg Ile Arg Gly Lys Lys Ala Lys Val Asn Phe Pro Glu Ala Pro Gly145 150 155 160Thr Ser Ser Val Lys Arg Ser Lys Val Asn Pro Gln Glu Asn Leu Lys 165 170 175Thr Val Gln Pro Asn Leu Gly His Lys Phe Ser Ala Gly Asn Asn His 180 185 190Met Asp Leu Val Glu Gln Lys Pro Leu Val Ser Gln Tyr Ala Asn Met 195 200 205Ala Ser Phe Pro Gly Ser Gly Asn Gly Leu Arg Ser Leu Pro Ser Ser 210 215 220Asp Asp Ala Thr Leu Tyr Phe Ser Ser Asp Gln Gly Ser Asn Ser Phe225 230 235 240Asp Tyr Ala Pro Glu Ile Ser Ser Met Leu Ser Ala Pro Leu Asp Cys 245 250 255Glu Ser His Phe Val Gln Asn Ala Asn Gln Gln Gln Pro Asn Ser Gln 260 265 270Asn Val Val Ser Ile Glu Asp Asp Ser Ala Lys Thr Leu Ser Glu Glu 275 280 285Leu Val Asp Ile Glu Ser Glu Leu Lys Phe Phe Gln Met Pro Tyr Leu 290 295 300Glu Gly Ser Trp Gly Asp Thr Ser Leu Glu Ser Leu Leu Ser Gly Asp305 310 315 320Thr Thr Gln Asp Gly Gly Asn Leu Met Asn Leu Trp Cys Leu Asp Asp 325 330 335Ile Pro Ser Met Ala Gly Gly Val Phe 340 34571185PRTZea mays 71Met Ala Glu Ala Pro Ala Ser Pro Gly Gly Gly Gly Gly Ser His Glu1 5 10 15Ser Gly Ser Pro Arg Gly Gly Gly Gly Gly Gly Ser Val Arg Glu Gln 20 25 30Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala 35 40 45Ile Pro Ala Asn Gly Lys Thr Ile Pro Ala Asn Gly Lys Ile Ala Lys 50 55 60Asp Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu Phe Ile Ser Phe65 70 75 80Ile Thr Ser Glu Ala Ser Asp Lys Cys Gln Arg Glu Lys Arg Lys Thr 85 90 95Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Ala Thr Leu Gly Phe Glu 100 105 110Asp Tyr Ile Glu Pro Leu Lys Val Tyr Leu Gln Lys Tyr Arg Glu Met 115 120 125Glu Gly Asp Ser Lys Leu Thr Ala Lys Ser Ser Asp Gly Ser Ile Lys 130 135 140Lys Asp Ala Leu Gly His Val Gly Ala Ser Ser Ser Ala Ala Gln Gly145 150 155 160Met Gly Gln Gln Gly Ala Tyr Asn Gln Gly Met Gly Tyr Met Gln Pro 165 170 175Gln Tyr His Asn Gly Asp Ile Ser Asn 180 18572355PRTArabidopsis thaliana 72Ile Tyr His Arg Lys Val Val Phe Thr Tyr Val Arg Ala Lys Arg Phe1 5 10 15Tyr His Phe Leu Asn Ile Glu Met Val Thr Asp Phe Lys Ser Leu Leu 20 25 30Pro Val Ile Asp Ile Ser Pro Leu Leu Ala Lys Cys Asp Asp Phe Asp 35 40 45Met Ala Glu Asp Ala Gly Val Val Glu Val Val Gly Lys Leu Asp Arg 50 55 60Ala Cys Arg Asp Val Gly Phe Phe Tyr Val Ile Gly His Gly Ile Ser65 70 75 80Asp Asp Leu Ile Asn Lys Val Lys Glu Met Thr His Gln Phe Phe Glu 85 90 95Leu Pro Tyr Glu Glu Lys Leu Lys Ile Lys Ile Thr Pro Thr Ala Gly 100 105 110Tyr Arg Gly Tyr Gln Arg Ile Gly Val Asn Phe Thr Ser Gly Lys Gln 115 120 125Asp Met His Glu Ala Ile Asp Cys Tyr Arg Glu Phe Lys Gln Gly Lys 130 135 140His Gly Asp Ile Gly Lys Val Leu Glu Gly Pro Asn Gln Trp Pro Gly145 150 155 160Asn Pro Gln Glu Tyr Lys Asp Leu Met Glu Lys Tyr Ile Lys Leu Cys 165 170 175Thr Asp Leu Ser Arg Asn Ile Leu Arg Gly Ile Ser Leu Ala Leu Gly 180 185 190Gly Ser Pro Tyr Glu Phe Glu Gly Lys Met Leu Arg Asp Pro Phe Trp 195 200 205Val Met Arg Ile Ile Gly Tyr Pro Gly Val Asn Gln Glu Asn Val Ile 210 215 220Gly Cys Gly Ala His Thr Asp Tyr Gly Leu Leu Thr Leu Ile Asn Gln225 230 235 240Asp Asp Asp Lys Thr Ala Leu Gln Val Lys Asn Val Asp Gly Asp Trp 245 250 255Ile Pro Ala Ile Pro Ile Pro Gly Ser Phe Ile Cys Asn Ile Gly Asp 260 265 270Met Leu Thr Ile Leu Ser Asn Gly Val Tyr Gln Ser Thr Leu His Lys 275 280 285Val Ile Asn Asn Ser Pro Lys Tyr Arg Val Cys Val Ala Phe Phe Tyr 290 295 300Glu Thr Asn Phe Glu Ala Glu Val Glu Pro Leu Asp Ile Phe Lys Glu305 310 315 320Lys His Pro Arg Lys Glu Thr Ser Gln Val Ala Lys Arg Val Val Tyr 325 330 335Gly Gln His Leu Ile Asn Lys Val Leu Thr Thr Phe Ala Asn Leu Val 340 345 350Glu Asn Ser 35573131PRTArabidopsis thaliana 73Met Asp Gln Gly Gly Arg Ser Ser Gly Ser Gly Gly Gly Gly Ala Glu1 5 10 15Gln Gly Lys Tyr Arg Gly Val Arg Arg Arg Pro Trp Gly Lys Tyr Ala 20 25 30Ala Glu Ile Arg Asp Ser Arg Lys His Gly Glu Arg Val Trp Leu Gly 35 40 45Thr Phe Asp Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Arg Ala Ala 50 55 60Tyr Ser Met Arg Gly Lys Ala Ala Ile Leu Asn Phe Pro His Glu Asn65 70 75 80Asn Met Gly Thr Gly Ser Ser Ser Thr Ala Ala Asn Ser Ser Ser Ser 85 90 95Ser Gln Gln Val Phe Glu Phe Glu Tyr Leu Asp Asp Ser Val Leu Asp 100 105 110Glu Leu Leu Glu Tyr Gly Glu Asn Tyr Asn Lys Thr His Asn Ile Asn 115 120 125Met Gly Lys 13074286PRTOryza sativa Japonica

Group 74Met Gly Met Ala Asn Glu Glu Ser Pro Asn Tyr Gln Val Lys Lys Gly1 5 10 15Gly Arg Ile Pro Pro Arg Ser Ser Leu Ile Tyr Pro Phe Met Ser Met 20 25 30Gly Pro Ala Ala Gly Glu Gly Cys Gly Leu Cys Gly Ala Asp Gly Gly 35 40 45Gly Cys Cys Ser Arg His Arg His Asp Asp Asp Gly Phe Pro Phe Val 50 55 60Phe Pro Pro Ser Ala Cys Gln Gly Ile Gly Ala Pro Ala Pro Pro Val65 70 75 80His Glu Phe Gln Phe Phe Gly Asn Asp Gly Gly Gly Asp Asp Gly Glu 85 90 95Ser Val Ala Trp Leu Phe Asp Asp Tyr Pro Pro Pro Ser Pro Val Ala 100 105 110Ala Ala Ala Gly Met His His Arg Gln Pro Pro Tyr Asp Gly Val Val 115 120 125Ala Pro Pro Ser Leu Phe Arg Arg Asn Thr Gly Ala Gly Gly Leu Thr 130 135 140Phe Asp Val Ser Leu Gly Glu Arg Pro Asp Leu Asp Ala Gly Leu Gly145 150 155 160Leu Gly Gly Gly Gly Gly Arg His Ala Glu Ala Ala Ala Ser Ala Thr 165 170 175Ile Met Ser Tyr Cys Gly Ser Thr Phe Thr Asp Ala Ala Ser Ser Met 180 185 190Pro Lys Glu Met Val Ala Ala Met Ala Asp Asp Gly Glu Ser Leu Asn 195 200 205Pro Asn Thr Val Val Gly Ala Met Val Glu Arg Glu Ala Lys Leu Met 210 215 220Arg Tyr Lys Glu Lys Arg Lys Lys Arg Cys Tyr Glu Lys Gln Ile Arg225 230 235 240Tyr Ala Ser Arg Lys Ala Tyr Ala Glu Met Arg Pro Arg Val Arg Gly 245 250 255Arg Phe Ala Lys Glu Pro Asp Gln Glu Ala Val Ala Pro Pro Ser Thr 260 265 270Tyr Val Asp Pro Ser Arg Leu Glu Leu Gly Gln Trp Phe Arg 275 280 28575272PRTZea mays 75Met Ala Pro Gln Ser Leu Asp Leu Gly Leu Ser Leu Gly Leu Gly Val1 5 10 15Ala Ala Phe Gln Pro Ser Ser Phe Cys His Pro Gly Asn Ala Val Val 20 25 30Val Pro Ala Ala Ala Glu Arg Glu Ala Ser Pro Ala Ala Ala Glu Glu 35 40 45Arg Glu Arg Arg Cys Ser Pro Ala Gly Ser Pro Val Ser Ser Gly Ser 50 55 60Gly Ser Gly Asn Lys Arg Ala Ala Ala Glu Arg Ser Ala Gly Ala Gly65 70 75 80Ala Gly Ser Gly Asp Glu Asp Asp Asp Gly Ala Ala Arg Lys Lys Leu 85 90 95Arg Leu Ser Lys Asp Gln Ala Ala Val Leu Glu Glu Cys Phe Lys Thr 100 105 110His His Thr Leu Thr Pro Lys Gln Lys Val Ala Leu Ala Ser Ser Leu 115 120 125Gly Leu Arg Pro Arg Gln Val Glu Val Trp Phe Gln Asn Arg Arg Ala 130 135 140Arg Thr Lys Leu Lys Gln Thr Glu Val Asp Cys Glu Tyr Leu Lys Arg145 150 155 160Trp Cys Glu Gln Leu Ala Glu Glu Asn Arg Arg Leu Gly Lys Glu Val 165 170 175Ala Glu Leu Arg Ala Leu Ser Ala Ala Pro Ala Ala Pro Leu Thr Thr 180 185 190Leu Thr Met Cys Leu Ser Cys Arg Arg Val Ala Ser Ser Ser Pro Ser 195 200 205Ser Ser Ser Ser Pro Arg Pro Ser Ile Pro Gly Ala Ala Ala Ala Ser 210 215 220Gly Gly Ser Met Ala Ser Pro Ala Ala Ala Ala Thr Leu Pro Ala His225 230 235 240Arg Gln Phe Phe Cys Gly Phe Arg Asp Ala Gly Ala Ala Ala Ala Ala 245 250 255Tyr Gly Thr Ala Ser Ala Gly Leu Ala Lys Pro Val Arg Ala Ala Arg 260 265 27076594PRTZea mays 76Met Val Lys Arg Ser Lys Lys Ser Lys Ser Lys Arg Val Thr Leu Arg1 5 10 15Gln Lys His Lys Val Gln Arg Lys Val Lys Glu His His Arg Lys Lys 20 25 30Arg Lys Glu Ala Lys Lys Ala Gly Lys Ala Gly Gln Arg Arg Lys Val 35 40 45Glu Lys Asp Pro Gly Ile Pro Asn Glu Trp Pro Phe Lys Glu Gln Glu 50 55 60Leu Lys Ala Leu Glu Ala Arg Arg Ala Gln Ala Leu Gln Glu Leu Glu65 70 75 80Leu Lys Lys Gln Ala Arg Lys Glu Arg Ala Gln Lys Arg Lys Ala Gly 85 90 95Leu Leu Glu Asp Glu Asp Ile Ala Ser Leu Ala Ser Ala Ala Ser Ala 100 105 110Gln Gly Ser Glu Phe Ala Ala Lys Glu Asn Ala Pro Leu Leu Val Ala 115 120 125Lys Ile Asn Asp His Ser Glu Arg Ser Phe Tyr Lys Glu Leu Val Lys 130 135 140Val Ile Glu Ala Ser Asp Val Ile Met Glu Val Leu Asp Ala Arg Asp145 150 155 160Pro Leu Gly Thr Arg Cys Ile Asp Met Glu Lys Met Val Arg Lys Ala 165 170 175Asp Pro Ser Lys Arg Ile Val Leu Leu Leu Asn Lys Ile Asp Leu Val 180 185 190Pro Lys Glu Ala Val Glu Lys Trp Leu Thr Tyr Leu Arg Glu Glu Leu 195 200 205Pro Thr Val Ala Phe Lys Cys Asn Thr Gln Glu Gln Arg Thr Lys Leu 210 215 220Gly Trp Lys Ser Ser Lys Leu Asp Lys Thr Ser Asn Ile Pro Gln Ser225 230 235 240Ser Asp Cys Leu Gly Ala Glu Asn Leu Ile Lys Leu Leu Lys Asn Tyr 245 250 255Ser Arg Ser His Glu Leu Lys Leu Ala Ile Thr Val Gly Ile Val Gly 260 265 270Leu Pro Asn Val Gly Lys Ser Ser Leu Ile Asn Ser Leu Lys Arg Ser 275 280 285Arg Val Val Asn Val Gly Ser Thr Pro Gly Ile Thr Arg Ser Met Gln 290 295 300Glu Val Gln Leu Asp Lys Lys Val Lys Leu Leu Asp Cys Pro Gly Val305 310 315 320Val Met Leu Lys Ser Ser Asn Ser Gly Val Ser Val Ala Leu Arg Asn 325 330 335Cys Lys Arg Val Glu Lys Ile Glu Asp Pro Val Ala Pro Val Lys Glu 340 345 350Ile Leu Ser Ile Cys Pro His Glu Lys Leu Leu Ser Leu Tyr Lys Val 355 360 365Pro Asn Phe Gly Ser Val Asp Asp Phe Leu Gln Lys Val Ala Thr Val 370 375 380Arg Gly Lys Leu Lys Lys Gly Gly Val Val Asp Val Glu Ala Ala Ala385 390 395 400Arg Ile Val Leu His Asp Trp Asn Glu Gly Lys Ile Pro Tyr Phe Thr 405 410 415Leu Pro Pro Lys Arg Asp Ala Gly Glu Asp Ser Asp Ala Val Ile Ile 420 425 430Ser Glu Asp Gly Lys Glu Phe Asn Ile Asp Glu Ile Tyr Lys Ala Glu 435 440 445Ser Ser Tyr Ile Gly Gly Leu Lys Ser Ile Glu Glu Phe His His Ile 450 455 460Glu Ile Pro Pro Asn Ala Pro Leu Ala Ile Asp Glu Glu Met Leu Glu465 470 475 480Asp Gly Gly Lys Lys Pro Ser Glu Ala Ile Gln Glu Ser Arg Asp Arg 485 490 495Glu Glu Gln Met Pro Asp Val Lys Asp Ser Gly Gly Ser Lys Ala Ala 500 505 510Ser Ala Ser Thr Gln Asn Asp Lys Leu Tyr Thr Ala Asp Ala Ile Leu 515 520 525Asp Pro His Lys Arg Lys Ala Glu Lys Lys Arg Arg Lys Ala Ser Arg 530 535 540Pro Ser Ala Leu Asn Asp Met Asp Ala Asp Tyr Asp Phe Lys Val Asp545 550 555 560Tyr Arg Met Glu Asp Gly Gly Ser Glu Gly Ala His Ala Asp Asp Glu 565 570 575Asp Gly Gly Asp Gly Ser Glu Asp Asn Glu Pro Met Thr Gly Val Asp 580 585 590Asp Ala77133PRTArabidopsis thaliana 77Met Asp Gln Gly Gly Arg Ser Ser Gly Ser Gly Gly Gly Gly Ala Glu1 5 10 15Gln Gly Lys Tyr Arg Gly Val Arg Arg Arg Pro Trp Gly Lys Tyr Ala 20 25 30Ala Glu Ile Arg Asp Ser Arg Lys His Gly Glu Arg Val Trp Leu Gly 35 40 45Thr Phe Asp Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Arg Ala Ala 50 55 60Tyr Ser Met Arg Gly Lys Ala Ala Ile Leu Asn Phe Pro His Glu Tyr65 70 75 80Asn Met Gly Thr Gly Ser Ser Ser Thr Ala Ala Asn Ser Ser Ser Ser 85 90 95Ser Gln Gln Val Phe Glu Phe Glu Tyr Leu Asp Asp Ser Val Leu Asp 100 105 110Glu Leu Leu Glu Tyr Gly Glu Asn Tyr Asn Lys Thr His Asn Ile Asn 115 120 125Met Gly Lys Arg Gln 13078497PRTArabidopsis thaliana 78Met Ile Ile Tyr Ile Leu Leu Leu Leu Asn Arg Thr Ser His His Arg1 5 10 15Asp Ser Ala Ser Asn Ser Ser Asn His Lys Cys Gln Gln Gln Lys Pro 20 25 30Arg Lys Asp Lys Gln Lys Gln Val Glu Gln Asn Thr Lys Lys Ile Glu 35 40 45Glu His Gln Ile Lys Ser Glu Ser Thr Leu Leu Ile Ser Asn His Asn 50 55 60Val Asn Met Ser Ser Gln Ser Asn Asn Ser Glu Ser Thr Ser Thr Asn65 70 75 80Asn Ser Ser Lys Pro His Thr Gly Gly Asp Ile Arg Trp Asp Ala Val 85 90 95Asn Ser Leu Lys Ser Arg Gly Ile Lys Leu Gly Ile Ser Asp Phe Arg 100 105 110Val Leu Lys Arg Leu Gly Tyr Gly Asp Ile Gly Ser Val Tyr Leu Val 115 120 125Glu Leu Lys Gly Ala Asn Pro Thr Thr Tyr Phe Ala Met Lys Val Met 130 135 140Asp Lys Ala Ser Leu Val Ser Arg Asn Lys Leu Leu Arg Ala Gln Thr145 150 155 160Glu Arg Glu Ile Leu Ser Gln Leu Asp His Pro Phe Leu Pro Thr Leu 165 170 175Tyr Ser His Phe Glu Thr Asp Lys Phe Tyr Cys Leu Val Met Glu Phe 180 185 190Cys Ser Gly Gly Asn Leu Tyr Ser Leu Arg Gln Lys Gln Pro Asn Lys 195 200 205Cys Phe Thr Glu Asp Ala Ala Arg Phe Phe Ala Ser Glu Val Leu Leu 210 215 220Ala Leu Glu Tyr Leu His Met Leu Gly Ile Val Tyr Arg Asp Leu Lys225 230 235 240Pro Glu Asn Val Leu Val Arg Asp Asp Gly His Ile Met Leu Ser Asp 245 250 255Phe Asp Leu Ser Leu Arg Cys Ser Val Asn Pro Thr Leu Val Lys Ser 260 265 270Phe Asn Gly Gly Gly Thr Thr Gly Ile Ile Asp Asp Asn Ala Ala Val 275 280 285Gln Gly Cys Tyr Gln Pro Ser Ala Phe Phe Pro Arg Met Leu Gln Ser 290 295 300Ser Lys Lys Asn Arg Lys Ser Lys Ser Asp Phe Asp Gly Ser Leu Pro305 310 315 320Glu Leu Met Ala Glu Pro Thr Asn Val Lys Ser Met Ser Phe Val Gly 325 330 335Thr His Glu Tyr Leu Ala Pro Glu Ile Ile Lys Asn Glu Gly His Gly 340 345 350Ser Ala Val Asp Trp Trp Thr Phe Gly Ile Phe Ile Tyr Glu Leu Leu 355 360 365His Gly Ala Thr Pro Phe Lys Gly Gln Gly Asn Lys Ala Thr Leu Tyr 370 375 380Asn Val Ile Gly Gln Pro Leu Arg Phe Pro Glu Tyr Ser Gln Val Ser385 390 395 400Ser Thr Ala Lys Asp Leu Ile Lys Gly Leu Leu Val Lys Glu Pro Gln 405 410 415Asn Arg Ile Ala Tyr Lys Arg Gly Ala Thr Glu Ile Lys Gln His Pro 420 425 430Phe Phe Glu Gly Val Asn Trp Ala Leu Ile Arg Gly Glu Thr Pro Pro 435 440 445His Leu Pro Glu Pro Val Asp Phe Ser Cys Tyr Val Lys Lys Glu Lys 450 455 460Glu Ser Leu Pro Pro Ala Ala Thr Glu Lys Lys Ser Lys Met Phe Asp465 470 475 480Glu Ala Asn Lys Ser Gly Ser Asp Pro Asp Tyr Ile Val Phe Glu Tyr 485 490 495Phe79131PRTArabidopsis thaliana 79Met Asp Gln Gly Gly Arg Ser Ser Gly Ser Gly Gly Gly Gly Ala Glu1 5 10 15Gln Gly Lys Tyr Arg Gly Val Arg Arg Arg Pro Trp Gly Lys Tyr Ala 20 25 30Ala Glu Ile Arg Asp Ser Arg Lys His Gly Glu Arg Val Trp Leu Gly 35 40 45Thr Phe Asp Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Arg Ala Ala 50 55 60Tyr Ser Met Arg Gly Lys Ala Ala Ile Leu Asn Phe Pro His Glu Tyr65 70 75 80Asn Met Gly Thr Gly Ser Ser Ser Thr Ala Ala Asn Ser Ser Ser Ser 85 90 95Ser Gln Gln Val Phe Glu Phe Glu Tyr Leu Asp Asp Ser Val Leu Asp 100 105 110Glu Leu Leu Glu Tyr Gly Glu Asn Tyr Asn Lys Thr His Asn Ile Asn 115 120 125Val Gly Lys 13080690PRTArabidopsis thaliana 80Met Asp Ala Thr Glu Gln Ser Leu Arg Gln Ser Leu Ser Glu Lys Ser1 5 10 15Ser Ser Val Glu Ala Gln Gly Asn Ala Val Arg Ala Leu Lys Ala Ser 20 25 30Arg Ala Ala Lys Pro Glu Ile Asp Ala Ala Ile Glu Gln Leu Asn Lys 35 40 45Leu Lys Leu Glu Lys Ser Thr Val Glu Lys Glu Leu Gln Ser Ile Ile 50 55 60Ser Ser Ser Gly Asn Gly Ser Leu Asn Arg Glu Ala Phe Arg Lys Ala65 70 75 80Val Val Asn Thr Leu Glu Arg Arg Leu Phe Tyr Ile Pro Ser Phe Lys 85 90 95Ile Tyr Ser Gly Val Ala Gly Leu Phe Asp Tyr Gly Pro Pro Gly Cys 100 105 110Ala Ile Lys Ser Asn Val Leu Ser Phe Trp Arg Gln His Phe Ile Leu 115 120 125Glu Glu Asn Met Leu Glu Val Asp Cys Pro Cys Val Thr Pro Glu Val 130 135 140Val Leu Lys Ala Ser Gly His Val Asp Lys Phe Thr Asp Leu Met Val145 150 155 160Lys Asp Glu Lys Thr Gly Thr Cys Tyr Arg Ala Asp His Leu Leu Lys 165 170 175Asp Tyr Cys Thr Glu Lys Leu Glu Lys Asp Leu Thr Ile Ser Ala Glu 180 185 190Lys Ala Ala Glu Leu Lys Asp Val Leu Ala Val Met Glu Asp Phe Ser 195 200 205Pro Glu Gln Leu Gly Ala Lys Ile Arg Glu Tyr Gly Ile Thr Ala Pro 210 215 220Asp Thr Lys Asn Pro Leu Ser Asp Pro Tyr Pro Phe Asn Leu Met Phe225 230 235 240Gln Thr Ser Ile Gly Pro Ser Gly Leu Ile Pro Gly Tyr Met Arg Pro 245 250 255Glu Thr Ala Gln Gly Ile Phe Val Asn Phe Lys Asp Leu Tyr Tyr Tyr 260 265 270Asn Gly Lys Lys Leu Pro Phe Ala Ala Ala Gln Ile Gly Gln Ala Phe 275 280 285Arg Asn Glu Ile Ser Pro Arg Gln Gly Leu Leu Arg Val Arg Glu Phe 290 295 300Thr Leu Ala Glu Ile Glu His Phe Val Asp Pro Glu Asn Lys Ser His305 310 315 320Pro Lys Phe Ser Asp Val Ala Lys Leu Glu Phe Leu Met Phe Pro Arg 325 330 335Glu Glu Gln Met Ser Gly Gln Ser Ala Lys Lys Leu Cys Leu Gly Glu 340 345 350Ala Val Ala Lys Gly Thr Val Asn Asn Glu Thr Leu Gly Tyr Phe Ile 355 360 365Gly Arg Val Tyr Leu Phe Leu Thr Arg Leu Gly Ile Asp Lys Glu Arg 370 375 380Leu Arg Phe Arg Gln His Leu Ala Asn Glu Met Ala His Tyr Ala Ala385 390 395 400Asp Cys Trp Asp Ala Glu Ile Glu Ser Ser Tyr Gly Trp Ile Glu Cys 405 410 415Val Gly Ile Ala Asp Arg Ser Ala Tyr Asp Leu Arg Ala His Ser Asp 420 425 430Lys Ser Gly Thr Pro Leu Val Ala Glu Glu Lys Phe Ala Glu Pro Lys 435 440 445Glu Val Glu Lys Leu Val Ile Thr Pro Val Lys Lys Glu Leu Gly Leu 450 455 460Ala Phe Lys Gly Asn Gln Lys Asn Val Val Glu Ser Leu Glu Ala Met465 470 475 480Asn Glu Glu Glu Ala Met Glu Met Lys Ala Thr Leu Glu Ser Lys Gly 485 490 495Glu Val Glu Phe Tyr Val Cys Thr Leu Lys Lys Ser Val Asn Ile Lys 500 505 510Lys Asn Met Val Ser Ile Ser Lys Glu Lys Lys Lys Glu His Gln Arg 515 520 525Val Phe Thr Pro Ser Val Ile Glu Pro Ser Phe Gly Ile Gly Arg Ile 530

535 540Ile Tyr Cys Leu Tyr Glu His Cys Phe Ser Thr Arg Pro Ser Lys Ala545 550 555 560Gly Asp Glu Gln Leu Asn Leu Phe Arg Phe Pro Pro Leu Val Ala Pro 565 570 575Ile Lys Cys Thr Val Phe Pro Leu Val Gln Asn Gln Gln Phe Glu Glu 580 585 590Ala Ala Lys Val Ile Ser Lys Glu Leu Ala Ser Val Gly Ile Ser His 595 600 605Lys Ile Asp Ile Thr Gly Thr Ser Ile Gly Lys Arg Tyr Ala Arg Thr 610 615 620Asp Glu Leu Gly Val Pro Phe Ala Ile Thr Val Asp Ser Asp Thr Ser625 630 635 640Val Thr Ile Arg Glu Arg Asp Ser Lys Asp Gln Val Arg Val Thr Leu 645 650 655Lys Glu Ala Ala Ser Val Val Ser Ser Val Ser Glu Gly Lys Met Thr 660 665 670Trp Gln Asp Val Trp Ala Thr Phe Pro His His Ser Ser Ala Ala Ala 675 680 685Asp Glu 69081460PRTArabidopsis thaliana 81Met Thr Val Val Gly Asp Val Ala Pro Ile Pro Arg Arg Asn Ser Ser1 5 10 15Thr Cys Ser Asn Asp Ile Ala Ala Pro Leu Leu Pro Glu Cys His Gly 20 25 30Asp Glu Val Ala His Asp Glu Phe Asn Gly Ala Ser Phe Ser Gly Ala 35 40 45Val Phe Asn Leu Ala Thr Thr Ile Ile Gly Ala Gly Ile Met Ala Leu 50 55 60Pro Ala Thr Met Lys Ile Leu Gly Leu Gly Leu Gly Ile Thr Met Ile65 70 75 80Val Val Met Ala Phe Leu Thr Asp Ala Ser Ile Glu Phe Leu Leu Arg 85 90 95Phe Ser Lys Ala Gly Lys Asn Arg Ser Tyr Gly Gly Leu Met Gly Gly 100 105 110Ser Phe Gly Asn Pro Gly Arg Ile Leu Leu Gln Val Ala Val Leu Val 115 120 125Asn Asn Ile Gly Val Leu Ile Val Tyr Met Ile Ile Ile Gly Asp Val 130 135 140Leu Ala Gly Lys Thr Glu Asp Gly Ile His His Phe Gly Val Leu Glu145 150 155 160Gly Trp Phe Gly His His Trp Trp Asn Gly Arg Ala Ala Ile Leu Leu 165 170 175Ile Thr Thr Leu Gly Val Phe Ala Pro Leu Ala Cys Phe Lys Arg Ile 180 185 190Asp Ser Leu Lys Phe Thr Ser Ala Leu Ser Val Ala Leu Ala Val Val 195 200 205Phe Leu Ile Ile Thr Ala Gly Ile Ser Ile Met Lys Leu Ile Ser Gly 210 215 220Gly Val Ala Met Pro Arg Leu Leu Pro Asp Val Thr Asp Leu Thr Ser225 230 235 240Phe Trp Asn Leu Phe Thr Val Val Pro Val Leu Val Thr Ala Phe Ile 245 250 255Cys His Tyr Asn Val His Ser Ile Gln Asn Glu Leu Glu Asp Pro Ser 260 265 270Gln Ile Arg Pro Val Val Arg Ser Ala Leu Met Leu Cys Ser Ser Val 275 280 285Tyr Ile Met Thr Ser Ile Phe Gly Phe Leu Leu Phe Gly Asp Asp Thr 290 295 300Leu Asp Asp Val Leu Ala Asn Phe Asp Thr Asp Leu Gly Ile Pro Phe305 310 315 320Gly Ser Ile Leu Asn Asp Ala Val Arg Val Ser Tyr Ala Leu His Leu 325 330 335Met Leu Val Phe Pro Ile Val Phe Cys Pro Leu Arg Ile Asn Ile Asp 340 345 350Gly Leu Leu Phe Pro Ser Ala Arg Ser Leu Ser Thr Ser Asn Val Arg 355 360 365Phe Gly Cys Leu Thr Ala Gly Leu Ile Ser Val Ile Phe Leu Gly Ala 370 375 380Asn Phe Ile Pro Ser Ile Trp Asp Ala Phe Gln Phe Thr Gly Ala Thr385 390 395 400Ala Ala Val Cys Leu Gly Phe Ile Phe Pro Ala Ser Ile Ile Leu Lys 405 410 415Asp Arg His Asp Lys Ala Thr Asn Arg Asp Thr Thr Leu Ala Ile Phe 420 425 430Met Ile Val Leu Ala Val Leu Ser Asn Ala Ile Ala Ile Tyr Ser Asp 435 440 445Ala Tyr Ala Leu Phe Lys Lys Asn Ala Pro Arg Glu 450 455 46082180PRTZea mays 82Met Ala Asp Ala Pro Ala Ser Pro Gly Gly Gly Gly Gly Ser His Glu1 5 10 15Ser Gly Ser Pro Arg Gly Gly Gly Gly Gly Gly Gly Gly Ser Val Arg 20 25 30Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys 35 40 45Lys Ala Ile Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr 50 55 60Val Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala65 70 75 80Ser Asp Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp 85 90 95Leu Leu Trp Ala Met Ala Thr Leu Gly Phe Glu Asp Tyr Ile Glu Pro 100 105 110Leu Lys Val Tyr Leu Gln Lys Tyr Arg Glu Met Glu Gly Asp Ser Lys 115 120 125Leu Thr Ser Lys Ser Ser Asp Gly Ser Ile Lys Lys Asp Ala Leu Gly 130 135 140His Val Gly Ala Ser Ser Ser Ala Val Gln Gly Met Gly Gln Gln Gly145 150 155 160Thr Tyr Asn Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His Asn Gly 165 170 175Asp Ile Ser Asn 18083971PRTZea mays 83Met Ile Val Gln Pro Ile Glu Leu Arg Ala Trp Thr Ala Phe Pro Gly1 5 10 15Ser Ala Gln Glu Gly Ile Gly Arg Met Ala Ala Ser Val Ser Arg Ala 20 25 30Ile Cys Val Gln Lys Pro Gly Ser Lys Cys Thr Arg Asp Arg Glu Ala 35 40 45Thr Ser Phe Ala Arg Arg Ser Val Ala Ala Pro Arg Pro Pro His Ala 50 55 60Lys Ala Ala Gly Val Ile Arg Ser Asp Ser Gly Ala Gly Arg Gly Gln65 70 75 80His Cys Ser Pro Leu Arg Ala Val Val Asp Ala Ala Pro Ile Gln Thr 85 90 95Thr Lys Lys Arg Val Phe His Phe Gly Lys Gly Lys Ser Glu Gly Asn 100 105 110Lys Thr Met Lys Glu Leu Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu 115 120 125Met Ala Ser Ile Gly Leu Ser Val Pro Pro Gly Phe Thr Val Ser Thr 130 135 140Glu Ala Cys Gln Gln Tyr Gln Asp Ala Gly Cys Ala Leu Pro Ala Gly145 150 155 160Leu Trp Ala Glu Ile Val Asp Gly Leu Gln Trp Val Glu Glu Tyr Met 165 170 175Gly Ala Thr Leu Gly Asp Pro Gln Arg Pro Leu Leu Leu Ser Val Arg 180 185 190Ser Gly Ala Ala Val Ser Met Pro Gly Met Met Asp Thr Val Leu Asn 195 200 205Leu Gly Leu Asn Asp Glu Val Ala Ala Gly Leu Ala Ala Lys Ser Gly 210 215 220Glu Arg Phe Ala Tyr Asp Ser Phe Arg Arg Phe Leu Asp Met Phe Gly225 230 235 240Asn Val Val Met Asp Ile Pro Arg Ser Leu Phe Glu Glu Lys Leu Glu 245 250 255His Met Lys Glu Ser Lys Gly Leu Lys Asn Asp Thr Asp Leu Thr Ala 260 265 270Ser Asp Leu Lys Glu Leu Val Gly Gln Tyr Lys Glu Val Tyr Leu Ser 275 280 285Ala Lys Gly Glu Pro Phe Pro Ser Asp Pro Lys Lys Gln Leu Glu Leu 290 295 300Ala Val Leu Ala Val Phe Asn Ser Trp Glu Ser Pro Arg Ala Lys Lys305 310 315 320Tyr Arg Ser Ile Asn Gln Ile Thr Gly Leu Arg Gly Thr Ala Val Asn 325 330 335Val Gln Cys Met Val Phe Gly Asn Met Gly Asn Thr Ser Gly Thr Gly 340 345 350Val Leu Phe Thr Arg Asn Pro Asn Thr Gly Glu Lys Lys Leu Tyr Gly 355 360 365Glu Phe Leu Val Asn Ala Gln Gly Glu Asp Val Val Ala Gly Ile Arg 370 375 380Thr Pro Glu Asp Leu Asp Ala Met Lys Asn Leu Met Pro Gln Ala Tyr385 390 395 400Asp Glu Leu Val Glu Asn Cys Asn Ile Leu Glu Ser His Tyr Lys Glu 405 410 415Met Gln Asp Ile Glu Phe Thr Val Gln Glu Asn Arg Leu Trp Met Leu 420 425 430Gln Cys Arg Thr Gly Lys Arg Thr Gly Lys Ser Ala Val Lys Ile Ala 435 440 445Val Asp Met Val Asn Glu Gly Leu Val Glu Pro Arg Ser Ala Ile Lys 450 455 460Met Val Glu Pro Gly His Leu Asp Gln Leu Leu His Pro Gln Phe Glu465 470 475 480Asn Pro Ser Ala Tyr Lys Asp Gln Val Ile Ala Thr Gly Leu Pro Ala 485 490 495Ser Pro Gly Ala Ala Val Gly Gln Val Val Phe Thr Ala Glu Asp Ala 500 505 510Glu Ala Trp His Ser Gln Gly Lys Ala Ala Ile Leu Val Arg Ala Glu 515 520 525Thr Ser Pro Glu Asp Val Gly Gly Met His Ala Ala Val Gly Ile Leu 530 535 540Thr Glu Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg Gly545 550 555 560Trp Gly Lys Cys Cys Val Ser Gly Cys Ser Gly Ile Arg Val Asn Asp 565 570 575Ala Glu Lys Leu Val Thr Ile Gly Gly His Val Leu Arg Glu Gly Glu 580 585 590Trp Leu Ser Leu Asn Gly Ser Thr Gly Glu Val Ile Leu Gly Lys Gln 595 600 605Pro Leu Ser Pro Pro Ala Leu Ser Gly Asp Leu Gly Thr Phe Met Ala 610 615 620Trp Val Asp Asp Val Arg Lys Leu Lys Val Leu Ala Asn Ala Asp Thr625 630 635 640Pro Asp Asp Ala Leu Thr Ala Arg Asn Asn Gly Ala Gln Gly Ile Gly 645 650 655Leu Cys Arg Thr Glu His Met Phe Phe Ala Ser Asp Glu Arg Ile Lys 660 665 670Ala Val Arg Gln Met Ile Met Ala Pro Thr Leu Glu Leu Arg Gln Gln 675 680 685Ala Leu Asp Arg Leu Leu Pro Tyr Gln Arg Ser Asp Phe Glu Gly Ile 690 695 700Phe Arg Ala Met Asp Gly Leu Pro Val Thr Ile Arg Leu Leu Asp Pro705 710 715 720Pro Leu His Glu Phe Leu Pro Glu Gly Asn Ile Glu Asp Ile Val Ser 725 730 735Glu Leu Cys Ala Glu Thr Gly Ala Asn Gln Glu Asp Ala Leu Ala Arg 740 745 750Ile Glu Lys Leu Ser Glu Val Asn Pro Met Leu Gly Phe Arg Gly Cys 755 760 765Arg Leu Gly Ile Ser Tyr Pro Glu Leu Thr Glu Met Gln Ala Arg Ala 770 775 780Ile Phe Glu Ala Ala Ile Ala Met Thr Asn Gln Gly Val Gln Val Phe785 790 795 800Pro Glu Ile Met Val Pro Leu Val Gly Thr Pro Gln Glu Leu Gly His 805 810 815Gln Val Thr Leu Ile Arg Gln Val Ala Glu Lys Val Phe Ala Asn Val 820 825 830Gly Lys Thr Ile Gly Tyr Lys Val Gly Thr Met Ile Glu Ile Pro Arg 835 840 845Ala Ala Leu Val Ala Asp Glu Ile Ala Glu Gln Ala Glu Phe Phe Ser 850 855 860Phe Gly Thr Asn Asp Leu Thr Gln Met Thr Phe Gly Tyr Ser Arg Asp865 870 875 880Asp Val Gly Lys Phe Ile Pro Val Tyr Leu Ala Gln Gly Ile Leu Gln 885 890 895His Asp Pro Phe Glu Val Leu Asp Gln Arg Gly Val Gly Glu Leu Val 900 905 910Lys Phe Ala Thr Glu Arg Gly Arg Lys Ala Arg Pro Asn Leu Lys Val 915 920 925Gly Ile Cys Gly Glu His Gly Gly Glu Pro Ser Ser Val Ala Phe Phe 930 935 940Ala Lys Ala Gly Leu Asp Tyr Val Ser Cys Ser Pro Phe Arg Val Pro945 950 955 960Ile Ala Arg Leu Ala Ala Ala Gln Val Leu Val 965 97084523PRTChlorella sorokiniana 84Cys Arg Pro Pro Ser Ser Pro Ser Leu Ser Trp Pro Pro Gly Leu Arg1 5 10 15Ser Pro Ala Leu Pro Arg Ala Val Ala Cys Ala Arg Gly Arg Ser Ala 20 25 30Lys Arg Asp Val Ala Ala Lys Arg Leu Arg Ser Arg Ser Pro Arg Met 35 40 45Asp Ala Thr Thr Gly Asp Phe Thr Ala Leu Gln Lys Ala Val Lys Gln 50 55 60Met Ala Thr Lys Ala Gly Thr Glu Gly Leu Val His Gly Ile Lys Asn65 70 75 80Pro Glu Leu Arg Gln Leu Leu Thr Glu Ile Phe Met Lys Asp Pro Glu 85 90 95Gln Gln Glu Phe Met Gln Ala Val Arg Glu Val Ala Val Ser Leu Gln 100 105 110Pro Val Phe Glu Lys Arg Pro Glu Leu Leu Pro Ile Phe Lys Gln Ile 115 120 125Val Glu Pro Glu Arg Val Ile Thr Phe Arg Val Ser Trp Leu Asp Asp 130 135 140Ala Gly Asn Leu Gln Val Asn Arg Gly Phe Arg Val Gln Tyr Ser Ser145 150 155 160Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn 165 170 175Leu Ser Ile Met Lys Phe Leu Ala Phe Glu Gln Ile Phe Lys Asn Ser 180 185 190Leu Thr Thr Leu Pro Met Gly Gly Gly Lys Gly Gly Ser Asp Phe Asp 195 200 205Pro Lys Gly Lys Ser Asp Ala Glu Val Met Arg Leu Trp Gln Ser Phe 210 215 220Met Thr Glu Leu Gln Arg His Ile Ser Tyr Val Gln Asp Val Pro Ala225 230 235 240Gly Asp Ile Gly Val Gly Ala Arg Glu Ile Gly Tyr Leu Phe Gly Gln 245 250 255Tyr Lys Arg Ile Thr Lys Asn Tyr Thr Gly Val Leu Thr Gly Lys Gly 260 265 270Gln Glu Tyr Gly Gly Ser Glu Ile Arg Pro Glu Ala Thr Gly Tyr Gly 275 280 285Ala Val Leu Phe Val Glu Asn Val Leu Lys Asp Lys Gly Glu Ser Leu 290 295 300Lys Gly Lys Arg Cys Leu Val Ser Gly Ala Gly Asn Val Ala Gln Tyr305 310 315 320Cys Ala Glu Leu Leu Leu Glu Lys Gly Ala Ile Val Leu Ser Leu Ser 325 330 335Asp Ser Gln Gly Tyr Val Tyr Glu Pro Asn Gly Phe Thr Arg Glu Gln 340 345 350Leu Gln Ala Val Gln Asp Met Lys Lys Lys Asn Asn Ser Ala Arg Ile 355 360 365Ser Glu Tyr Lys Ser Asp Thr Ala Val Tyr Val Gly Asp Arg Arg Lys 370 375 380Pro Trp Glu Leu Asp Cys Gln Val Asp Ile Ala Phe Pro Cys Ala Thr385 390 395 400Gln Asn Glu Ile Asp Glu His Asp Ala Glu Leu Leu Ile Lys His Gly 405 410 415Cys Gln Tyr Val Val Glu Gly Ala Asn Met Pro Ser Thr Asn Glu Ala 420 425 430Ile His Lys Tyr Asn Lys Ala Gly Ile Ile Tyr Cys Pro Gly Lys Ala 435 440 445Ala Asn Ala Gly Gly Val Ala Val Ser Gly Leu Glu Met Thr Gln Asn 450 455 460Arg Met Ser Leu Asn Trp Thr Arg Glu Glu Val Arg Asp Lys Leu Glu465 470 475 480Arg Ile Met Lys Asp Ile Tyr Asp Ser Ala Met Gly Pro Ser Arg Glu 485 490 495Tyr Asn Val Asp Leu Ala Ala Gly Ala Asn Ile Ala Gly Phe Thr Lys 500 505 510Val Ala Asp Ala Val Lys Ala Gln Gly Ala Val 515 52085947PRTZea mays 85Met Thr Ala Ser Val Ser Arg Ala Ile Cys Val Gln Lys Pro Gly Ser1 5 10 15Lys Cys Thr Arg Asp Arg Glu Ala Thr Ser Phe Ala Arg Arg Ser Val 20 25 30Ala Ala Pro Arg Pro Pro His Ala Lys Ala Ala Gly Val Ile Arg Ser 35 40 45Asp Ser Gly Ala Gly Arg Gly Gln His Cys Ser Pro Leu Arg Ala Val 50 55 60Val Asp Ala Ala Pro Ile Gln Thr Thr Lys Lys Arg Val Phe His Phe65 70 75 80Gly Lys Gly Lys Ser Glu Gly Asn Lys Thr Met Lys Glu Leu Leu Gly 85 90 95Gly Lys Gly Ala Asn Leu Ala Glu Met Ala Ser Ile Gly Leu Ser Val 100 105 110Pro Pro Gly Phe Thr Val Ser Thr Glu Ala Cys Gln Gln Tyr Gln Asp 115 120 125Ala Gly Cys Ala Leu Pro Ala Gly Leu Trp Ala Glu Ile Val Asp Gly 130 135 140Leu Gln Trp Val Glu Glu Tyr Met Gly Ala Thr Leu Gly Asp Pro Gln145 150 155 160Arg Pro Leu Leu Leu Ser Val Arg Ser Gly Ala Ala Val Ser Met Pro 165 170 175Gly Met Met Asp

Thr Val Leu Asn Leu Gly Leu Asn Asp Glu Val Ala 180 185 190Ala Gly Leu Ala Ala Lys Ser Gly Glu Arg Phe Ala Tyr Asp Ser Phe 195 200 205Arg Arg Phe Leu Asp Met Phe Gly Asn Val Val Met Asp Ile Pro Arg 210 215 220Ser Leu Phe Glu Glu Lys Leu Glu His Met Lys Glu Ser Lys Gly Leu225 230 235 240Lys Asn Asp Thr Asp Leu Thr Ala Ser Asp Leu Lys Glu Leu Val Gly 245 250 255Gln Tyr Lys Glu Val Tyr Leu Ser Ala Lys Gly Glu Pro Phe Pro Ser 260 265 270Asp Pro Lys Lys Gln Leu Glu Leu Ala Val Leu Ala Val Phe Asn Ser 275 280 285Trp Glu Ser Pro Arg Ala Lys Lys Tyr Arg Ser Ile Asn Gln Ile Thr 290 295 300Gly Leu Arg Gly Thr Ala Val Asn Val Gln Cys Met Val Phe Gly Asn305 310 315 320Met Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro Asn 325 330 335Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln Gly 340 345 350Glu Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp Ala Met 355 360 365Lys Asn Leu Met Pro Gln Ala Tyr Asp Glu Leu Val Glu Asn Cys Asn 370 375 380Ile Leu Glu Ser His Tyr Lys Glu Met Gln Asp Ile Glu Phe Thr Val385 390 395 400Gln Glu Asn Arg Leu Trp Met Leu Gln Cys Arg Thr Gly Lys Arg Thr 405 410 415Gly Lys Ser Ala Val Lys Ile Ala Val Asp Met Val Asn Glu Gly Leu 420 425 430Val Glu Pro Arg Ser Ala Ile Lys Met Val Glu Pro Gly His Leu Asp 435 440 445Gln Leu Leu His Pro Gln Phe Glu Asn Pro Ser Ala Tyr Lys Asp Gln 450 455 460Val Ile Ala Thr Gly Leu Pro Ala Ser Pro Gly Ala Ala Val Gly Gln465 470 475 480Val Val Phe Thr Ala Glu Asp Ala Glu Ala Trp His Ser Gln Gly Lys 485 490 495Ala Ala Ile Leu Val Arg Ala Glu Thr Ser Pro Glu Asp Val Gly Gly 500 505 510Met His Ala Ala Val Gly Ile Leu Thr Glu Arg Gly Gly Met Thr Ser 515 520 525His Ala Ala Val Val Ala Arg Gly Trp Gly Lys Cys Cys Val Ser Gly 530 535 540Cys Ser Gly Ile Arg Val Asn Asp Ala Glu Lys Leu Val Thr Ile Gly545 550 555 560Gly His Val Leu Arg Glu Gly Glu Trp Leu Ser Leu Asn Gly Ser Thr 565 570 575Gly Glu Val Ile Leu Gly Lys Gln Pro Leu Ser Pro Pro Ala Leu Ser 580 585 590Gly Asp Leu Gly Thr Phe Met Ala Trp Val Asp Asp Val Arg Lys Leu 595 600 605Lys Val Leu Ala Asn Ala Asp Thr Pro Asp Asp Ala Leu Thr Ala Arg 610 615 620Asn Asn Gly Ala Gln Gly Ile Gly Leu Cys Arg Thr Glu His Met Phe625 630 635 640Phe Ala Ser Asp Glu Arg Ile Lys Ala Val Arg Gln Met Ile Met Ala 645 650 655Pro Thr Leu Glu Leu Arg Gln Gln Ala Leu Asp Arg Leu Leu Pro Tyr 660 665 670Gln Arg Ser Asp Phe Glu Gly Ile Phe Arg Ala Met Asp Gly Leu Pro 675 680 685Val Thr Ile Arg Leu Leu Asp Pro Pro Leu His Glu Phe Leu Pro Glu 690 695 700Gly Asn Ile Glu Asp Ile Val Ser Glu Leu Cys Ala Glu Thr Gly Ala705 710 715 720Asn Gln Glu Asp Ala Leu Ala Arg Ile Glu Lys Leu Ser Glu Val Asn 725 730 735Pro Met Leu Gly Phe Arg Gly Cys Arg Leu Gly Ile Ser Tyr Pro Glu 740 745 750Leu Thr Glu Met Gln Ala Arg Ala Ile Phe Glu Ala Ala Ile Ala Met 755 760 765Thr Asn Gln Gly Val Gln Val Phe Pro Glu Ile Met Val Pro Leu Val 770 775 780Gly Thr Pro Gln Glu Leu Gly His Gln Val Thr Leu Ile Arg Gln Val785 790 795 800Ala Glu Lys Val Phe Ala Asn Val Gly Lys Thr Ile Gly Tyr Lys Val 805 810 815Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Leu Val Ala Asp Glu Ile 820 825 830Ala Glu Gln Ala Glu Phe Phe Ser Phe Gly Thr Asn Asp Leu Thr Gln 835 840 845Met Thr Phe Gly Tyr Ser Arg Asp Asp Val Gly Lys Phe Ile Pro Val 850 855 860Tyr Leu Ala Gln Gly Ile Leu Gln His Asp Pro Phe Glu Val Leu Asp865 870 875 880Gln Arg Gly Val Gly Glu Leu Val Lys Leu Ala Thr Glu Arg Gly Arg 885 890 895Lys Ala Arg Pro Asn Leu Lys Val Gly Ile Cys Gly Glu His Gly Gly 900 905 910Glu Pro Ser Ser Val Ala Phe Phe Ala Lys Ala Gly Leu Asp Tyr Val 915 920 925Ser Cys Ser Pro Phe Arg Val Pro Ile Ala Arg Leu Ala Ala Ala Gln 930 935 940Val Leu Val94586947PRTZea mays 86Met Ala Ala Ser Val Ser Arg Ala Ile Cys Val Gln Lys Pro Gly Ser1 5 10 15Lys Cys Thr Arg Asp Arg Glu Ala Thr Ser Phe Ala Arg Arg Ser Val 20 25 30Ala Ala Pro Arg Pro Pro His Ala Lys Ala Arg Arg Arg His Pro Leu 35 40 45Arg Leu Arg Arg Gly Thr Gly Pro His Cys Ser Pro Leu Arg Ala Val 50 55 60Val Asp Ala Ala Pro Ile Gln Thr Thr Lys Lys Arg Val Phe His Phe65 70 75 80Gly Lys Gly Lys Ser Glu Gly Asn Lys Thr Met Lys Glu Leu Leu Gly 85 90 95Gly Lys Gly Ala Asn Leu Ala Glu Met Ala Ser Ile Gly Leu Ser Val 100 105 110Pro Pro Gly Phe Thr Val Ser Thr Glu Ala Cys Gln Gln Tyr Gln Asp 115 120 125Ala Gly Cys Ala Leu Pro Ala Gly Leu Trp Ala Glu Ile Val Asp Gly 130 135 140Leu Gln Trp Val Glu Glu Tyr Met Gly Ala Thr Leu Gly Asp Pro Gln145 150 155 160Arg Pro Leu Leu Leu Ser Val Arg Ser Gly Ala Ala Val Ser Met Pro 165 170 175Gly Met Met Asp Thr Val Leu Asn Leu Gly Leu Asn Asp Glu Val Ala 180 185 190Ala Gly Leu Ala Ala Lys Ser Gly Glu Arg Phe Ala Tyr Asp Ser Phe 195 200 205Arg Arg Phe Leu Asp Met Phe Gly Asn Val Val Met Asp Ile Pro Arg 210 215 220Ser Leu Phe Glu Glu Lys Leu Glu His Met Lys Glu Ser Lys Gly Leu225 230 235 240Lys Asn Asp Thr Asp Leu Thr Ala Ser Asp Leu Lys Glu Leu Val Gly 245 250 255Gln Tyr Lys Glu Val Tyr Leu Ser Ala Lys Gly Glu Pro Phe Pro Ser 260 265 270Asp Pro Lys Lys Gln Leu Glu Leu Ala Val Leu Ala Val Phe Asn Ser 275 280 285Trp Glu Ser Pro Arg Ala Lys Lys Tyr Arg Ser Ile Asn Gln Ile Thr 290 295 300Gly Leu Arg Gly Thr Ala Val Asn Val Gln Cys Met Val Phe Gly Asn305 310 315 320Met Gly Asn Thr Ser Gly Thr Gly Val Leu Phe Thr Arg Asn Pro Asn 325 330 335Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu Val Asn Ala Gln Gly 340 345 350Glu Asp Val Val Ala Gly Ile Arg Thr Pro Glu Asp Leu Asp Ala Met 355 360 365Lys Asn Leu Met Pro Gln Ala Tyr Asp Glu Leu Val Glu Asn Cys Asn 370 375 380Ile Leu Glu Ser His Tyr Lys Glu Met Gln Asp Ile Glu Phe Thr Val385 390 395 400Gln Glu Asn Arg Leu Trp Met Leu Gln Cys Arg Thr Gly Lys Arg Thr 405 410 415Gly Lys Ser Ala Val Lys Ile Ala Val Asp Met Val Asn Glu Gly Leu 420 425 430Val Glu Pro Arg Ser Ala Ile Lys Met Val Glu Pro Gly His Leu Asp 435 440 445Gln Leu Leu His Pro Gln Phe Glu Asn Pro Ser Ala Tyr Lys Asp Gln 450 455 460Val Ile Ala Thr Gly Leu Pro Ala Ser Pro Gly Ala Ala Val Gly Gln465 470 475 480Val Val Phe Thr Ala Glu Asp Ala Glu Ala Trp His Ser Gln Gly Lys 485 490 495Ala Ala Ile Leu Val Arg Ala Glu Thr Ser Pro Glu Asp Val Gly Gly 500 505 510Met His Ala Ala Val Gly Ile Leu Thr Glu Arg Gly Gly Met Thr Ser 515 520 525His Ala Ala Val Val Ala Arg Trp Trp Gly Lys Cys Cys Val Ser Gly 530 535 540Cys Ser Gly Ile Arg Val Asn Asp Ala Glu Lys Leu Val Thr Ile Gly545 550 555 560Ser His Val Leu Arg Glu Gly Glu Trp Leu Ser Leu Asn Gly Ser Thr 565 570 575Gly Glu Val Ile Leu Gly Lys Gln Pro Leu Ser Pro Pro Ala Leu Ser 580 585 590Gly Asp Leu Gly Thr Phe Met Ala Trp Val Asp Asp Val Arg Lys Leu 595 600 605Lys Val Leu Ala Asn Ala Asp Thr Pro Asp Asp Ala Leu Thr Ala Arg 610 615 620Asn Asn Gly Ala Gln Gly Ile Gly Leu Cys Arg Thr Glu His Met Phe625 630 635 640Phe Ala Ser Asp Glu Arg Ile Lys Ala Val Arg Gln Met Ile Met Ala 645 650 655Pro Thr Leu Glu Leu Arg Gln Gln Ala Leu Asp Arg Leu Leu Thr Tyr 660 665 670Gln Arg Ser Asp Phe Glu Gly Ile Phe Arg Ala Met Asp Gly Leu Pro 675 680 685Val Thr Ile Arg Leu Leu Asp His Pro Ser Tyr Glu Phe Leu Pro Glu 690 695 700Gly Asn Ile Glu Asp Ile Val Ser Glu Leu Cys Ala Glu Thr Gly Ala705 710 715 720Asn Gln Glu Asp Ala Leu Ala Arg Ile Glu Lys Leu Ser Glu Val Asn 725 730 735Pro Met Leu Gly Phe Arg Gly Cys Arg Leu Gly Ile Ser Tyr Pro Glu 740 745 750Leu Thr Glu Met Gln Ala Arg Ala Ile Phe Glu Ala Ala Ile Ala Met 755 760 765Thr Asn Gln Gly Val Gln Val Phe Pro Glu Ile Met Val Pro Leu Val 770 775 780Gly Thr Pro Gln Glu Leu Gly His Gln Val Thr Leu Ile Arg Gln Val785 790 795 800Ala Glu Lys Val Phe Ala Asn Val Gly Lys Thr Ile Gly Tyr Lys Val 805 810 815Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Leu Val Ala Asp Glu Ile 820 825 830Ala Glu Gln Ala Glu Phe Phe Ser Phe Gly Thr Asn Asp Leu Thr Gln 835 840 845Met Thr Phe Gly Tyr Ser Arg Asp Asp Val Gly Lys Phe Ile Pro Val 850 855 860His Leu Ala Gln Gly Ile Leu Gln His Asp Pro Phe Glu Val Leu Asp865 870 875 880Gln Arg Gly Val Gly Glu Leu Val Lys Phe Ala Thr Glu Arg Gly Arg 885 890 895Lys Ala Arg Pro Asn Leu Lys Val Gly Ile Cys Gly Glu His Gly Gly 900 905 910Glu Pro Ser Ser Val Ala Phe Phe Ala Lys Ala Gly Leu Asp Phe Val 915 920 925Ser Cys Ser Pro Phe Arg Val Pro Ile Ala Arg Leu Ala Ala Ala Gln 930 935 940Val Leu Val94587282PRTArabidopsis thaliana 87Met Gly Thr Arg Ala Leu Gln Ile Pro Leu Leu Glu Gly Glu Thr Asp1 5 10 15Asn Tyr Asp Gly Val Thr Val Thr Met Val Glu Pro Met Asp Ser Glu 20 25 30Val Phe Thr Glu Ser Leu Arg Ala Ser Leu Ser His Trp Arg Glu Glu 35 40 45Gly Lys Lys Gly Ile Trp Ile Lys Leu Pro Leu Gly Leu Ala Asn Leu 50 55 60Val Glu Ala Ala Val Ser Glu Gly Phe Arg Tyr His His Ala Glu Pro65 70 75 80Glu Tyr Leu Met Leu Val Ser Trp Ile Ser Glu Thr Pro Asp Thr Ile 85 90 95Pro Ala Asn Ala Ser His Val Val Gly Ala Gly Ala Leu Val Ile Asn 100 105 110Lys Asn Thr Lys Glu Val Leu Val Val Gln Glu Arg Ser Gly Phe Phe 115 120 125Lys Asp Lys Asn Val Trp Lys Leu Pro Thr Gly Val Ile Asn Glu Gly 130 135 140Glu Asp Ile Trp Thr Gly Val Ala Arg Glu Val Glu Glu Glu Thr Gly145 150 155 160Ile Ile Ala Asp Phe Val Glu Val Leu Ala Phe Arg Gln Ser His Lys 165 170 175Ala Ile Leu Lys Lys Lys Thr Asp Met Phe Phe Leu Cys Val Leu Ser 180 185 190Pro Arg Ser Tyr Asp Ile Thr Glu Gln Lys Ser Glu Ile Leu Gln Ala 195 200 205Lys Trp Met Pro Ile Gln Glu Tyr Ile Asp Gln Pro Trp Asn Lys Lys 210 215 220Asn Glu Met Phe Lys Phe Met Ala Asn Ile Cys Gln Lys Lys Cys Glu225 230 235 240Glu Glu Tyr Leu Gly Phe Ala Ile Val Pro Thr Thr Thr Ser Ser Gly 245 250 255Lys Glu Ser Phe Ile Tyr Cys Asn Ala Asp His Ala Lys Arg Leu Lys 260 265 270Val Ser Arg Asp Gln Ala Ser Ala Ser Leu 275 28088567PRTArabidopsis thaliana 88Ile Val Leu Val Val Ala Val Leu Thr His Thr Ala Ser Ala Ala Val1 5 10 15Arg Glu Tyr His Trp Glu Val Glu Tyr Lys Tyr Trp Ser Pro Asp Cys 20 25 30Lys Glu Gly Ala Val Met Thr Val Asn Gly Glu Phe Pro Gly Pro Thr 35 40 45Ile Lys Ala Phe Ala Gly Asp Thr Ile Val Val Asn Leu Thr Asn Lys 50 55 60Leu Thr Thr Glu Gly Leu Val Ile His Trp His Gly Ile Arg Gln Phe65 70 75 80Gly Ser Pro Trp Ala Asp Gly Ala Ala Gly Val Thr Gln Cys Ala Ile 85 90 95Asn Pro Gly Glu Thr Phe Thr Tyr Asn Phe Thr Val Glu Lys Pro Gly 100 105 110Thr His Phe Tyr His Gly His Tyr Gly Met Gln Arg Ser Ala Gly Leu 115 120 125Tyr Gly Ser Leu Ile Val Asp Val Ala Lys Gly Lys Ser Glu Arg Leu 130 135 140Arg Tyr Asp Gly Glu Phe Asn Leu Leu Leu Ser Asp Trp Trp His Glu145 150 155 160Ala Ile Pro Ser Gln Glu Leu Gly Leu Ser Ser Lys Pro Met Arg Trp 165 170 175Ile Gly Glu Ala Gln Ser Ile Leu Ile Asn Gly Arg Gly Gln Phe Asn 180 185 190Cys Ser Leu Ala Ala Gln Phe Ser Asn Asn Thr Ser Leu Pro Met Cys 195 200 205Thr Phe Lys Glu Gly Asp Gln Cys Ala Pro Gln Ile Leu His Val Glu 210 215 220Pro Asn Lys Thr Tyr Arg Ile Arg Leu Ser Ser Thr Thr Ala Leu Ala225 230 235 240Ser Leu Asn Leu Ala Val Gln Gly His Lys Leu Val Val Val Glu Ala 245 250 255Asp Gly Asn Tyr Ile Thr Pro Phe Thr Thr Asp Asp Ile Asp Ile Tyr 260 265 270Ser Gly Glu Ser Tyr Ser Val Leu Leu Thr Thr Asp Gln Asp Pro Ser 275 280 285His Asn Tyr Tyr Ile Ser Val Gly Val Arg Gly Arg Lys Pro Asn Thr 290 295 300Thr Gln Ala Leu Thr Ile Leu Asn Tyr Val Thr Ala Pro Ala Ser Lys305 310 315 320Leu Pro Ser Ser Pro Pro Pro Val Thr Pro Arg Trp Asp Asp Phe Glu 325 330 335Arg Ser Lys Asn Phe Ser Lys Lys Ile Phe Ser Ala Met Gly Ser Pro 340 345 350Ser Pro Pro Lys Lys Tyr Arg Lys Arg Leu Ile Leu Leu Asn Thr Gln 355 360 365Asn Leu Ile Asp Gly Tyr Thr Lys Trp Ala Ile Asn Asn Val Ser Leu 370 375 380Val Thr Pro Ala Thr Pro Tyr Leu Gly Ser Val Lys Tyr Asn Leu Lys385 390 395 400Leu Gly Phe Asn Arg Lys Ser Pro Pro Arg Ser Tyr Arg Met Asp Tyr 405 410 415Asp Ile Met Asn Pro Pro Pro Phe Pro Asn Thr Thr Thr Gly Asn Gly 420 425 430Ile Tyr Val Phe Pro Phe Asn Val Thr Val Asp Val Ile Ile Gln Asn 435 440 445Ala Asn Val Leu Lys Gly Ile Val Ser Glu Ile His Pro Trp His Leu 450 455 460His Gly His Asp Phe Trp Val Leu Gly Tyr Gly Asp Gly Lys Phe

Lys465 470 475 480Pro Gly Ile Asp Glu Lys Thr Tyr Asn Leu Lys Asn Pro Pro Leu Arg 485 490 495Asn Thr Ala Ile Leu Tyr Pro Tyr Gly Trp Thr Ala Ile Arg Phe Val 500 505 510Thr Asp Asn Pro Gly Val Trp Phe Phe His Cys His Ile Glu Pro His 515 520 525Leu His Met Gly Met Gly Val Val Phe Ala Glu Gly Leu Asn Arg Ile 530 535 540Gly Lys Val Pro Asp Glu Ala Leu Gly Cys Gly Leu Thr Lys Gln Phe545 550 555 560Leu Met Asn Arg Asn Arg Asn 56589523PRTChlorella sorokiniana 89Cys Arg Pro Pro Ser Ser Pro Ser Leu Ser Trp Pro Pro Gly Leu Arg1 5 10 15Ser Pro Ala Leu Pro Arg Ala Val Ala Cys Ala Arg Gly Arg Ser Ala 20 25 30Lys Arg Asp Val Ala Ala Lys Arg Leu Arg Ser Arg Ser Pro Arg Met 35 40 45Asp Ala Thr Thr Gly Asp Phe Thr Ala Leu Gln Lys Ala Val Lys Gln 50 55 60Met Ala Thr Lys Ala Gly Thr Glu Gly Leu Val His Gly Ile Lys Asn65 70 75 80Pro Glu Leu Arg Gln Leu Leu Thr Glu Ile Phe Met Lys Asp Pro Glu 85 90 95Gln Gln Glu Phe Met Gln Ala Val Arg Glu Val Ala Val Ser Leu Gln 100 105 110Pro Val Phe Glu Lys Arg Pro Glu Leu Leu Pro Ile Phe Lys Gln Ile 115 120 125Val Glu Pro Glu Arg Val Ile Thr Phe Arg Val Ser Trp Leu Asp Asp 130 135 140Ala Gly Asn Leu Gln Val Asn Arg Gly Phe Arg Val Gln Tyr Ser Ser145 150 155 160Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe His Pro Ser Val Asn 165 170 175Leu Ser Ile Met Lys Phe Leu Ala Phe Glu Gln Ile Phe Lys Asn Ser 180 185 190Leu Thr Thr Leu Pro Met Gly Gly Gly Lys Gly Gly Ser Asp Phe Asp 195 200 205Pro Lys Gly Lys Ser Asp Ala Glu Val Met Arg Phe Cys Gln Ser Phe 210 215 220Met Thr Glu Leu Gln Arg His Ile Ser Tyr Val Gln Asp Val Pro Ala225 230 235 240Gly Asp Ile Gly Val Gly Ala Arg Glu Ile Gly Tyr Leu Phe Gly Gln 245 250 255Tyr Lys Arg Ile Thr Lys Asn Tyr Thr Gly Val Leu Thr Gly Lys Gly 260 265 270Gln Glu Tyr Gly Gly Ser Glu Ile Arg Pro Glu Ala Thr Gly Tyr Gly 275 280 285Ala Val Leu Phe Val Glu Asn Val Leu Lys Asp Lys Gly Glu Ser Leu 290 295 300Lys Gly Lys Arg Cys Leu Val Ser Gly Ala Gly Asn Val Ala Gln Tyr305 310 315 320Cys Ala Glu Leu Leu Leu Glu Lys Gly Ala Ile Val Leu Ser Leu Ser 325 330 335Asp Ser Gln Gly Tyr Val Tyr Glu Pro Asn Gly Phe Thr Arg Glu Gln 340 345 350Leu Gln Ala Val Gln Asp Met Lys Lys Lys Asn Asn Ser Ala Arg Ile 355 360 365Ser Glu Tyr Lys Ser Asp Thr Ala Val Tyr Val Gly Asp Arg Arg Lys 370 375 380Pro Trp Glu Leu Asp Cys Gln Val Asp Ile Ala Phe Pro Cys Ala Thr385 390 395 400Gln Asn Glu Ile Asp Glu His Asp Ala Glu Leu Leu Ile Lys His Gly 405 410 415Cys Gln Tyr Val Val Glu Gly Ala Asn Met Pro Ser Thr Asn Glu Ala 420 425 430Ile His Lys Tyr Asn Lys Ala Gly Ile Ile Tyr Cys Pro Gly Lys Ala 435 440 445Ala Asn Ala Gly Gly Val Ala Val Ser Gly Leu Glu Met Thr Gln Asn 450 455 460Arg Met Ser Leu Asn Trp Thr Arg Glu Glu Val Arg Asp Lys Leu Glu465 470 475 480Arg Ile Met Lys Asp Ile Tyr Asp Ser Ala Met Gly Pro Ser Arg Glu 485 490 495Tyr Asn Val Asp Leu Ala Ala Gly Ala Asn Ile Ala Gly Phe Thr Lys 500 505 510Val Ala Asp Ala Val Lys Ala Gln Gly Ala Val 515 52090131PRTArabidopsis thaliana 90Met Asp Gln Gly Gly Arg Ser Ser Gly Ser Gly Gly Gly Gly Ala Glu1 5 10 15Gln Gly Lys Tyr Arg Gly Val Arg Arg Arg Pro Trp Gly Lys Tyr Ala 20 25 30Ala Glu Ile Arg Asp Ser Arg Lys His Gly Glu Arg Val Trp Leu Gly 35 40 45Thr Phe Asp Thr Ala Glu Asp Ala Ala Arg Ala Tyr Asp Arg Ala Ala 50 55 60Tyr Ser Met Arg Gly Lys Ala Ala Ile Leu Asn Phe Pro His Glu Tyr65 70 75 80Asn Met Gly Thr Gly Ser Ser Ser Thr Ala Ala Asn Ser Ser Ser Ser 85 90 95Ser Gln Gln Val Phe Glu Phe Glu Tyr Leu Asp Asp Ser Val Leu Asp 100 105 110Glu Leu Leu Glu Tyr Gly Glu Asn Tyr Asn Lys Thr His Asn Ile Asn 115 120 125Met Gly Lys 13091423PRTZea mays 91Met Ala Gln Ala Val Val Pro Ala Met Gln Cys Arg Val Gly Val Lys1 5 10 15Ala Ala Ala Gly Arg Val Trp Ser Ala Gly Arg Thr Arg Thr Gly Arg 20 25 30Gly Gly Ala Ser Pro Gly Phe Lys Val Met Ala Val Ser Thr Gly Ser 35 40 45Thr Gly Val Val Pro Arg Leu Glu Gln Leu Leu Asn Met Asp Thr Thr 50 55 60Pro Tyr Thr Asp Lys Val Ile Ala Glu Tyr Ile Trp Val Gly Gly Ser65 70 75 80Gly Ile Asp Ile Arg Ser Lys Ser Arg Thr Ile Ser Lys Pro Val Glu 85 90 95Asp Pro Ser Glu Leu Pro Lys Trp Asn Tyr Asp Gly Ser Ser Thr Gly 100 105 110Gln Ala Pro Gly Glu Asp Ser Glu Val Ile Leu Tyr Pro Gln Ala Ile 115 120 125Phe Lys Asp Pro Phe Arg Gly Gly Asn Asn Val Leu Val Ile Cys Asp 130 135 140Thr Tyr Thr Pro Gln Gly Glu Pro Leu Pro Thr Asn Lys Arg His Arg145 150 155 160Ala Ala Gln Ile Phe Ser Asp Pro Lys Val Gly Glu Gln Val Pro Trp 165 170 175Phe Gly Ile Glu Gln Glu Tyr Thr Leu Leu Gln Lys Asp Val Asn Trp 180 185 190Pro Leu Gly Trp Pro Val Gly Gly Phe Pro Gly Pro Gln Gly Pro Tyr 195 200 205Tyr Cys Ala Val Gly Ala Asp Lys Ser Phe Gly Arg Asp Ile Ser Asp 210 215 220Ala His Tyr Lys Ala Cys Leu Tyr Ala Gly Ile Asn Ile Ser Gly Thr225 230 235 240Asn Gly Glu Val Met Pro Gly Gln Trp Glu Tyr Gln Val Gly Pro Ser 245 250 255Val Gly Ile Glu Ala Gly Asp His Ile Trp Ile Ser Arg Tyr Ile Leu 260 265 270Glu Arg Ile Thr Glu Gln Ala Gly Val Val Leu Thr Leu Asp Pro Lys 275 280 285Pro Ile Gln Gly Asp Trp Asn Gly Ala Gly Cys His Thr Asn Tyr Ser 290 295 300Thr Lys Thr Met Arg Glu Asp Gly Gly Phe Glu Glu Ile Lys Arg Ala305 310 315 320Ile Leu Asn Leu Ser Leu Arg His Asp Leu His Ile Ser Ala Tyr Gly 325 330 335Glu Gly Asn Glu Arg Arg Leu Thr Gly Lys His Glu Thr Ala Ser Ile 340 345 350Gly Thr Phe Ser Trp Gly Val Ala Asn Arg Gly Cys Ser Ile Arg Val 355 360 365Gly Arg Asp Thr Glu Ala Lys Gly Lys Gly Tyr Leu Glu Asp Arg Arg 370 375 380Pro Ala Ser Asn Met Asp Pro Tyr Ile Val Thr Gly Leu Leu Ala Glu385 390 395 400Thr Thr Ile Leu Trp Gln Pro Ser Leu Glu Ala Glu Ala Leu Ala Ala 405 410 415Lys Lys Leu Ala Leu Lys Val 42092263PRTZea mays 92Met Leu Glu Leu Arg Leu Val Gln Gly Ser Leu Leu Lys Lys Val Leu1 5 10 15Glu Ala Ile Arg Glu Leu Val Asn Asp Ala Asn Phe Asp Cys Ser Gly 20 25 30Thr Gly Phe Ser Leu Gln Ala Met Asp Ser Ser His Val Ala Leu Val 35 40 45Ala Leu Leu Leu Arg Ala Glu Gly Phe Glu His Tyr Arg Cys Asp Arg 50 55 60Asn Leu Ser Met Gly Met Asn Leu Asn Asn Met Ala Lys Met Leu Arg65 70 75 80Cys Ala Gly Asn Asp Asp Ile Ile Thr Ile Lys Ala Asp Asp Gly Ser 85 90 95Asp Thr Val Thr Phe Met Phe Glu Ser Pro Lys Gln Asp Lys Ile Ala 100 105 110Asp Phe Glu Met Lys Leu Met Asp Ile Asp Ser Glu His Leu Gly Ile 115 120 125Pro Asp Ser Glu Tyr Gln Ala Ile Val Arg Met Pro Ser Ser Glu Phe 130 135 140Met Arg Ile Cys Lys Asp Leu Ser Ser Ile Gly Asp Thr Val Val Ile145 150 155 160Ser Val Thr Lys Glu Gly Val Lys Phe Ser Thr Ser Gly Glu Ile Gly 165 170 175Ser Ala Asn Ile Val Cys Arg Gln Asn Gln Thr Ile Asp Lys Pro Glu 180 185 190Glu Ala Thr Ile Ile Glu Met Gln Glu Pro Val Ser Leu Thr Phe Ala 195 200 205Leu Arg Tyr Met Asn Ser Phe Thr Lys Ala Ser Ser Leu Ser Glu Gln 210 215 220Val Thr Ile Ser Leu Ser Ser Glu Leu Pro Val Val Val Glu Tyr Lys225 230 235 240Ile Ala Glu Met Gly Tyr Ile Arg Phe Tyr Leu Ala Pro Lys Ile Glu 245 250 255Asp Asp Glu Glu Met Lys Pro 260931388DNAArtificial Sequenceseed preferred promoter 93atcaacaaat tactcctcaa tcacactcct atagaaaacg gtttaagcta tcattacatg 60tctagttggt tttactcagc cctagaagtg ttgtttattg catcactttc cacgaagcac 120aatttttctt ttttacaatc accagacctc acaggctcac acatatgctt tagagcacat 180tctaaacttt gaactataaa agctgttaac actaatacac tatgcgttct tttttgctcc 240aaacactttt gatccattat taggagacac tccacttaga aagattttct aatcctttgg 300tcaactagga agttcaaggt ttttctaaac agaaattcat ttcacaagta atttaattta 360taaggaaatg aatagagaaa tcaaatcatt gaagaactac aaaatataga ttcaaggtca 420ggtctaagaa aatattcctg aagctcaaaa aagagttttc ctctcacatt atagaattgg 480cctttacttc aacattttcc cacctattcc acatttggtc agaacatttt taattacttg 540tggatcaatt tccggttgaa atgggtttgg tgaatatccg gttcagttat atggtggccg 600ttggaattgg cttattagtt gtggccgttg ttgaagccgt tggtattggt aagggagaag 660cagacttgtg gctatgagtc tatgaccatg actcgtgatt atggagctgt cttatgaccc 720tgaccatcac cttgatctgg tggattccaa tgttttcttc ttcttctaat aaaatattat 780ggtcaataca ggtgctaatt aagatggtaa taatttctta tgtttctgtg gtaaagtttg 840attcaattcc gtagttttag ataatcttat ttccatacat aaattttata gttttatcta 900ctttgttctt atgttttatc tctagccaag agttattatt attatcagaa gaagaaaaaa 960aaaagaagca tatatacaaa aggtttaata aaatgtatta tacaaggcaa ttatccaaat 1020tttttttgtt ttggtttaca ttgatgctct caggatttca taaggataga gagatctatt 1080cgtatacgtg tcacgtcatg agtgggtgtt tcgccaatcc atgaaacgca cctagatatc 1140taaaacacat atcaattgcg aatctgcgaa gtgcgagcca ttaaccacgt aagcaaacaa 1200acaatctaaa ccccaaaaaa aatctatgac tagccaatag caacctcaga gattgatatt 1260tcaagataag acagtattta gatttctgta ttatatatag cgaaaatcgc atcaatacca 1320aaccacccat ttcttggctt acaacaacaa atcttaaacg ttttactttg tgctgcacta 1380ctcaacct 1388941785DNAArtificial Sequenceroot preferred promoter 94taggaaaaaa gtttatattc ctacccaaac tttcggcacc agagacaaat taggtttgtt 60cagaaaatca gtcgctcatc gacagactta acaccacaaa atcatcagaa tttctttggg 120acaaaatgga aaatgttctt cacggctcca cctaccaaaa tttcgatatc aagctcaaaa 180gcatacacaa actataacta attccatagt tacgtaatct taacttcgaa ttcaacaaca 240catgcatgca tcgactgaat cttcaacaaa tgcaatcaaa cacacaaaat tgctaccaaa 300aaaatatgat ttttttttta tgatttcaat tttcatccgg cacttagtcc aaaacttttt 360ttgtgtgtca acatttttta aaaaaatctt taaacggata tctgatctaa gagcatgttc 420ataggtgata cttacaaaat atttttaaga aattttttag tattatttat aatgtttgtt 480taataaatat atataagatt ttttgctttt atcaaatgtg accaatcaga agaaaccacg 540tcagatgata ctgatatgac aaatatgata ctgatcaaac atattctaat tgctttacta 600atataaaaat aatttttgga cttgtgatac tctaaaaata tcacccatat acatggtcta 660atatatggat cgtaaaaaac tcatatataa tattaataag tagtagaaga gcgtagacca 720tgtcctgggt cgtcgtccaa atgaccacaa gaagatttca aaacagagga aaatatttct 780cattaaataa gttttcctga cgcataagat aacattatta caagattcag aaaaagaaag 840gtgaaaggat aatgtttctc ctactatata agatgtgtac atctgaaaaa atatgaatat 900atttgtaacg tttgactgtt attacatgat taatacgata taaatattaa catttttttt 960caaaataaaa gtaatatagt aaggaaatga aaagaggcat gaagcatgcc tctttttttg 1020gtcggctgcc gtttacaatt gccaattgcg atagttactc ttcttgcgtg tacgactttt 1080gttttttttt acatattcgc caataatttg acgttttcta ttagtttgtt tgatactctg 1140ttgtcttgct aaaactcaat aaaacattaa attactttct tgaatgaagc tggaacaaat 1200ctaacataaa tagaaaatga tgggcaagtt gatgttattc gtaaatttat ttagattata 1260ttatataaaa agcaatccaa ttatatatct catatataca atttcttatc ttactttgtc 1320aatgtcatat acgtaactaa aacttgcgga aatagaaaat gccacgtgta tggtggacat 1380aatccgaatc tctctctttc ttctataaat agtggccatt cccattggtt gaaatcacaa 1440aagcatcata agaagaagaa gaaactacaa tagttaatca atcaaagaga agtaagagaa 1500caggtaaacc cctagattct ctcttcttta catttatatg catatatgta gactatgtag 1560ttcgagcttc atggtacaaa attcaataaa ctcttcttat gacttcgttt acaattgtgt 1620ttgtgaatag acacaaaaga ttagttttgt ttactttaga ttcaaaacac ttcgggccta 1680tcctgtatat atgctgatca gatgcatgtg gttgattaat ctttaatctc atcatattaa 1740ttactaattt tttgtttgtt tgattaattt gtgtgtggta aaggt 1785951243DNAArtificial Sequencemeristem and endosperm preferred promoter 95aaaaccaccg agggacctga tctgcaccgg ttttgatagt tgagggaccc gttgtgtctg 60gttttccgat cgagggacga aaatcggatt cggtgtaaag ttaagggacc tcagatgaac 120ttattccgga gcatgattgg gaagggagga cataaggccc atgtcgcatg tgtttggacg 180gtccagatct ccagatcact cagcaggatc ggccgcgttc gcgtagcacc cgcggtttga 240ttcggcttcc cgcaaggcgg cggccggtgg ccgtgccgcc gtagcttccg ccggaagcga 300gcacgccgcc gccgccgacc cggctctgcg tttgcaccgc cttgcacgcg atacatcggg 360atagatagct actactctct ccgtttcaca atgtaaatca ttctactatt ttccacattc 420atattgatgt taatgaatat agacatatat atctatttag attcattaac atcaatatga 480atgtaggaaa tgctagaatg acttacattg tgaattgtga aatggacgaa gtacctacga 540tggatggatg caggatcatg aaagaattaa tgcaagatcg tatctgccgc atgcaaaatc 600ttactaattg cgctgcatat atgcatgaca gcctgcatgc gggcgtgtaa gcgtgttcat 660ccattaggaa gtaaccttgt cattacttat accagtacta catactatat agtattgatt 720tcatgagcaa atctacaaaa ctggaaagca ataaggaata cgggactgga aaagactcaa 780cattaatcac caaatatttc gccttctcca gcagaatata tatctctcca tcttgatcac 840tgtacacact gacagtgtac gcataaacgc agcagccagc ttaactgtcg tctcaccgtc 900gcacactggc cttccatctc aggctagctt tctcagccac ccatcgtaca tgtcaactcg 960gcgcgcgcac aggcacaaat tacgtacaaa acgcatgacc aaatcaaaac caccggagaa 1020gaatcgctcc cgcgcgcggc ggcggcgcgc acgtacgaat gcacgcacgc acgcccaacc 1080ccacgacacg atcgcgcgcg acgccggcga caccggccat ccacccgcgc cctcacctcg 1140ccgactataa atacgtaggc atctgcttga tcttgtcatc catctcacca ccaaaaaaaa 1200aggaaaaaaa aacaaaacac accaagccaa ataaaagcga caa 1243962439DNAArtificial Sequenceroot specific promoter 96ggaagctaac tagtcacggc gaatacatga cgacatcggc ctacaacgca caacttcttg 60gcataaaagc ttcaatttca atgcccctat ctggaagccc taggcgccgc gcaaatgtaa 120aacattcgct tcgcttggct tgttatccaa aatagagtat ggacctccga cagattggca 180acccgtgggt aatcgaaaat ggctccatct gcccctttgt cgaaggaatc aggaaacggc 240cctcacctcc tggcggagtg tagatatgtg aaagaatcta ggcgacactt gcagactgga 300caacatgtga acaaataaga ccaacgttat ggcaacaagc ctcgacgcta ctcaagtggt 360gggaggccac cgcatgttcc aacgaagcgc caaagaaagc cttgcagact ctaatgctat 420tagtcgccta ggatatttgg aatgaaagga accgcagagt ttttcagcac caagagcttc 480cggtggctag tctgatagcc aaaattaagg aggatgccaa aacatgggtc ttggcgggcg 540cgaaacacct tgataggtgg cttacctttt aacatgttcg ggccaaaggc cttgagacgg 600taaagttttc tatttgcgct tgcgcatgta caattttatt cctctattca atgaaattgg 660tggctcactg gttcattaaa aaaaaaagaa tctagcctgt tcgggaagaa gaggatttta 720ttcgtgagag agagagagag agagagagag agagggagag agaaggagga ggaggatttt 780caggcttcgc attgcccaac ctctgcttct gttggcccaa gaagaatccc aggcgcccat 840gggctggcag tttaccacgg acctacctag cctaccttag ctatctaagc gggccgacct 900agtagctacg tgcctagtgt agattaaagt tggcgggcca gcaggaagcc acgctgcaat 960ggcatcttcc cctgtccttc gcgtacgtga aaacaaaccc aggtaagctt agaatcttct 1020tgcccgttgg actgggacac ccaccaatcc caccatgccc cgatattcct ccggtctcgg 1080ttcatgtgat gtcctctctt gtgtgatcac ggagcaagca ttcttaaacg gcaaaagaaa 1140atcaccaact tgctcacgca gtcacgctgc accgcgcgaa gcgacgcccg ataggccaag 1200atcgcgagat aaaataacaa ccaatgatca taaggaaaca agcccgcgat gtgtcgtgtg 1260cagcaatctt ggtcatttgc gggatcgagt gcttcacggc taaccaaata ttcggccgat 1320gatttaacac attatcagcg tagatgtacg tacgatttgt taattaatct acgagccttg 1380ctagggcagg tgttctgcca gccaatccag atcgccctcg tatgcacgct cacatgatgg 1440cagggcaggg ttcacatgag ctctaacggt cgattaatta atcccggggc tcgactataa 1500atacctccct aatcccatga tcaaaaccat ctcaagcagc ctaatcatct ccagctgatc 1560aagagctctt

aattagctag ctagtgatta gctgcgcttg tgatcgatcg atctcgggta 1620cgtagcaata gatctaccgt cttcggtacg cgctcactcc gccctctgcc tttgttactg 1680ccacgtttct ctgaatgctc tcttgtgtgg tgattgctga gagtggttta gctggatcta 1740gaattacact ctgaaatcgt gttctgcctg tgctgattac ttgccgtcct ttgtagcagc 1800aaaatatagg gacatggtag tacgaaacga agatagaacc tacacagcaa tacgagaaat 1860gtgtaatttg gtgcttagcg gtatttattt aagcacatgt tggtgttata gggcacttgg 1920attcagaagt ttgctgttaa tttaggcaca ggcttcatac tacatgggtc aatagtatag 1980ggattcatat tataggcgat actataataa tttgttcgtc tgcagagctt attatttgcc 2040aaaattagat attcctattc tgtttttgtt tgtgtgctgt taaattgtta acgcctgaag 2100gaataaatat aaatgacgaa attttgatgt ttatctctgc tcctttattg tgaccataag 2160tcaagatcag atgcacttgt tttaaatatt gttgtctgaa gaaataagta ctgacagtat 2220tttgatgcat tgatctgctt gtttgttgta acaaaattta aaaataaaga gtttcctttt 2280tgttgctctc cttacctcct gatggtatct agtatctacc aactgacact atattgcttc 2340tctttacata cgtatcttgc tcgatgcctt ctccctagtg ttgaccagtg ttactcacat 2400agtctttgct catttcattg taatgcagat accaagcgg 2439972327DNAArtificial Sequenceendosperm specific promoter 97tgtttggact ccagaaaatt tacgggagtt ggtggagcag gtcattaagt actataaaaa 60atcatgtagc tgaagctgca agtatttaga agacatttag ataagttatt ttatttatca 120tttagattaa gaaaatttaa aactatttaa attgatatta taaactacag ctccacactg 180gagctagatc ctggagtcat tacaaacacc cccttaatgg gaaaagagaa gataatgtat 240atctaattat tgtttctgtg tcacctatag ctattagttc aaaacttcat aatcactggt 300acaaataagc tctagagagg cggttcggaa cccattttta ttgttgtttt tcaaaaccac 360tagtgttagg gaccgccagt ggaaactgaa acgccattgg aaattgattt tcactgatgg 420tgagctaaga aaaccgccat tggtaatcct ttgcagaaaa cataaactag gttttaaaaa 480tagtaaacaa atatttttat taggagaggc cccacatagt cgcaccattt ttcgcgcatt 540attcacgcgc tacgcaacca atggtaattg aacctcagag acttcactct tgtgtagcct 600cctttgccac tccactaaac acttacttgt gtcttgattg cattttgttg cccacatatt 660agaacaaaca gagtgtaaat tgattgtttg aggctgtaaa caaattcaaa tgaaaaagta 720gtcaactact aaattgaata attgtttatg ttctaccact tttattttgg tacttttccc 780atcggaggcg gtttgtaaaa tttgcatttt aagttttaca aatttcaatg aaattttgag 840agcccaaatg atttcaaata aaaaagttgt caactacaat gttttataac ttttaatttg 900gtggtttttt aaacaagctc atttgaaaaa ctaaaatgat cgattctaca tgatttttag 960gtcgattttt taaggaatcg cctgtacaaa tatttctact gacagttttt aagaaaccac 1020ctgtggaaat catagatttg tactagcggt ttttctcaag taactgctag tagaaatatg 1080gtggttttct taagaaaact gtttgtagga atgcacgatt tatataaatg gatttgttaa 1140gaaaaccgct agtggaatgt tctttcaact aacggttatt gagtcgtgac agccaattta 1200atttccttga taactaaaag cggctgtaaa aattagacca tgatgtaggc acggagctgt 1260tttgtactga atgcgcccac tgttttgttg gaaaagtgca tgtacttatt attcattctg 1320tttatttcta gctggcattc agttcttaca gccacagatt atgcaaaacg cctatttctg 1380ccagcaaatt tacaggaaaa gtcatggact tttccgggtt attttcctat aagtacagcc 1440attcctttca cttacaggcc ccaacattag cacaaagaac acaatagacc actgatttaa 1500cacctgcagg accaggtggg cccaccgtct tcggtacgcg ctcactccgc cctctgcctt 1560tgttactgcc acgtttctct gaatgctctc ttgtgtggtg attgctgaga gtggtttagc 1620tggatctaga attacactct gaaatcgtgt tctgcctgtg ctgattactt gccgtccttt 1680gtagcagcaa aatataggga catggtagta cgaaacgaag atagaaccta cacagcaata 1740cgagaaatgt gtaatttggt gcttagcggt atttatttaa gcacatgttg gtgttatagg 1800gcacttggat tcagaagttt gctgttaatt taggcacagg cttcatacta catgggtcaa 1860tagtataggg attcatatta taggcgatac tataataatt tgttcgtctg cagagcttat 1920tatttgccaa aattagatat tcctattctg tttttgtttg tgtgctgtta aattgttaac 1980gcctgaagga ataaatataa atgacgaaat tttgatgttt atctctgctc ctttattgtg 2040accataagtc aagatcagat gcacttgttt taaatattgt tgtctgaaga aataagtact 2100gacagtattt tgatgcattg atctgcttgt ttgttgtaac aaaatttaaa aataaagagt 2160ttcctttttg ttgctctcct tacctcctga tggtatctag tatctaccaa ctgacactat 2220attgcttctc tttacatacg tatcttgctc gatgccttct ccctagtgtt gaccagtgtt 2280actcacatag tctttgctca tttcattgta atgcagatac caagcgg 2327981713DNAArtificial Sequenceendosperm specific promoter 98atatagattt cttaacgaca atctcgcatt gtttattgcc tcacatggtg acacacagct 60cttacatttg tccttatact cacggtgcag tgcactacta ctgtaacccc atggtctgga 120aaacagcaac gcaacctttc tgccttggaa cagcaagcta tacagcgcta tataacaaac 180ttgcacaata atatgctaca tttctgaaag caaactccgc ttatcttcgc gttaaggaat 240ggtattacac ttaaaatgac aaaatatcac actttagaac cccacgcctt tacctaaaaa 300aaagttgatt gatacaagaa caaatgttta cagcttgtta cacctcccat caagcaaaga 360aatggaatct tatatagatt tcttaacgac aatctcgcat tgtttattgc ctcacatggt 420gacacacagc tcttacattt gtccttatac tcacggtgca gtgcactact actgtaaccc 480catggtctgg aaaacagcaa cgcaaccttt ctgccttgga acagcaagct atacagcgct 540atataacaaa cttgcacaat aatatgctac atttctgaaa gcaaactccg cttatcttcg 600cgttaaggaa tggtattaca cttaaaatga caaaatatca cactttagaa ccccacgcct 660ttacctaaaa aaaagttgat tgatacaaga acaaatgttt acagcttgtt acacctccca 720tcaagcaaag aaatggaatc ttttatttta tgtacacgtg tacgggtagc tattgtttat 780aaattgcaga agacacctca acatgatgga taattgtata cgcaaaacta ctttcctcga 840attccccatt ctataaattc cagttaaact taattcgtaa gatcaatcat aattctcgag 900ttgcaaacca ccgtcttcgg tacgcgctca ctccgccctc tgcctttgtt actgccacgt 960ttctctgaat gctctcttgt gtggtgattg ctgagagtgg tttagctgga tctagaatta 1020cactctgaaa tcgtgttctg cctgtgctga ttacttgccg tcctttgtag cagcaaaata 1080tagggacatg gtagtacgaa acgaagatag aacctacaca gcaatacgag aaatgtgtaa 1140tttggtgctt agcggtattt atttaagcac atgttggtgt tatagggcac ttggattcag 1200aagtttgctg ttaatttagg cacaggcttc atactacatg ggtcaatagt atagggattc 1260atattatagg cgatactata ataatttgtt cgtctgcaga gcttattatt tgccaaaatt 1320agatattcct attctgtttt tgtttgtgtg ctgttaaatt gttaacgcct gaaggaataa 1380atataaatga cgaaattttg atgtttatct ctgctccttt attgtgacca taagtcaaga 1440tcagatgcac ttgttttaaa tattgttgtc tgaagaaata agtactgaca gtattttgat 1500gcattgatct gcttgtttgt tgtaacaaaa tttaaaaata aagagtttcc tttttgttgc 1560tctccttacc tcctgatggt atctagtatc taccaactga cactatattg cttctcttta 1620catacgtatc ttgctcgatg ccttctccct agtgttgacc agtgttactc acatagtctt 1680tgctcatttc attgtaatgc agataccaag cgg 1713992516DNAArtificial Sequenceseed specific promoter 99acacttttat tatcgcgtca aatcagtacc tcaatcgata ttgtagccta gtgttcttat 60taaatgggaa gaattcgagg acacactaat tccttgctaa cacacactta tgctccattt 120ggatgtcgat attggagggc atggaactga attggtttca attacaaatc agccatgata 180ttgtaatgag atgtaatttc aattctattc tttggatgtc actgaattgg agtttggaat 240tgtgtggtcc aattccacct tatatagaag agggatgctc tgtattggga gagtgagttt 300ctagttatag tctagcttcg ggaaattgag tctctcgttc caaatctcaa ttccatgtgc 360aaccaaacaa tagaattctg gaaagctgat tccaattcct aattccgtgc tccaatatct 420acatccaaac gggtgttaca taaatataga aatgacatat caaccatgca aaaccacatt 480ggcgatgttg aacaaaggcg aacacccaca tactatgtac cgcacacggc atctctttct 540caaaggtcga accacgtgtg ttccatgcat gcgtggaaca tgcaaggttg tcacgtatag 600ggaatgatga cacacgagag cgcctacaag gcaacaaaca ccttacgtac cacgtagagt 660gcattttgct accacctgcc accggatgac atgtatgcat gcatgcgttg tgtacgcata 720cactgctgtc tgctggtgcc caaagaccat ctagaacagc atcttttaat tctccatttc 780cctcacgcca ttgctagtgc cttgcacatg ctcgcactcc ctaacacatc ttcctccctt 840tatttttcgt tgccaattgc tagttgttca aatgccacgt tttccttaca cagctgtagg 900gcaccgtacc acgtagaatg cattcctcgc caccaacaga caacacggcc gggcatatgt 960acgtcttacg ccggaccatc accagtatat atgatgctag ggatcagtgg gcgccctttt 1020tgcctcgtcc tcccggggcg gcattcctat gtcctaactg aagcaaccca cgcgccgcca 1080tttctgttgc gaatgagtcc atggacatat gtgccaacag aacccctcgg aaggcaccat 1140ctatctatct atctctcaag caatattata tttggcacct acgctcaagt acatagacag 1200tgtgcacggc attgtgcagc tggaaagccc gcccgacacg agggctgcca aatcgacagc 1260tccgcgccct tggaaatcct agtcacttgt tcacaattga ccaatctacc cttgaagcac 1320acggtggatg gtactgccac atttggctta taggggcata gaggacaatg aatgcaactg 1380gagcgggaag gagagcttta atttgtaagt actcggtgaa cacggcacct gatgatgatg 1440atgatggaca gcgaggaatt gttataaaag gcgcccgtcc ctcccatggc tcaagaacaa 1500gggaatcgaa gccattccct cttcaagagg ggatcatcag attgggctta ttattcctta 1560ttactccagg taattcttag tttgttgccc ttccaaacct ttacatctca tataagaatg 1620attattacat gcaagattat gttgacatgc gtcgtcatgg tatttttttt aggcaaggat 1680cggagttgct ctgaattgac tgaaccagat ctaccgtctt cggtacgcgc tcactccgcc 1740ctctgccttt gttactgcca cgtttctctg aatgctctct tgtgtggtga ttgctgagag 1800tggtttagct ggatctagaa ttacactctg aaatcgtgtt ctgcctgtgc tgattacttg 1860ccgtcctttg tagcagcaaa atatagggac atggtagtac gaaacgaaga tagaacctac 1920acagcaatac gagaaatgtg taatttggtg cttagcggta tttatttaag cacatgttgg 1980tgttataggg cacttggatt cagaagtttg ctgttaattt aggcacaggc ttcatactac 2040atgggtcaat agtataggga ttcatattat aggcgatact ataataattt gttcgtctgc 2100agagcttatt atttgccaaa attagatatt cctattctgt ttttgtttgt gtgctgttaa 2160attgttaacg cctgaaggaa taaatataaa tgacgaaatt ttgatgttta tctctgctcc 2220tttattgtga ccataagtca agatcagatg cacttgtttt aaatattgtt gtctgaagaa 2280ataagtactg acagtatttt gatgcattga tctgcttgtt tgttgtaaca aaatttaaaa 2340ataaagagtt tcctttttgt tgctctcctt acctcctgat ggtatctagt atctaccaac 2400tgacactata ttgcttctct ttacatacgt atcttgctcg atgccttctc cctagtgttg 2460accagtgtta ctcacatagt ctttgctcat ttcattgtaa tgcagatacc aagcgg 25161002282DNAArtificial Sequenceseed preferred promoter 100gaggtgcata aggctggcaa gacgacagtt aggaacgcat gcgagcgagg ttgacggacg 60cgataaggtt agcgcatgcg tcgaacgcgg gctggcgagg gtggaaggca tgataaggct 120attgggtagc gcaaaatgtg tagacagcgg gcgagtgagg atggcagtgg tggcatgcat 180ggacgcggtt ggagcatacg cgacaagaat ggagcacgac gtagatttcg ggaggccgtg 240gttggagcgc ccgcgggcga gatggcggcc atggttagag cgcccgtggt tacgggtggg 300ttcatggcga gcggaggggt tgcaagattt ccagggcgct cgggtcggtt gcaagctcca 360cggtggaggc gtgacggaga cgacgtgggg agggaggtcg tggggaaatt cggacgagca 420gaggcgtggc aggtgtggca tggggaggga ggtcgcgggg agggcgcagg gaggtggcat 480ggggagggag gctggggacg aagatgatgt gggcccagag ggacgcggga caaagaattg 540cgtatgataa cgggttgatt cgtagaattt taggcggtat ttataaaaat gacgcaggac 600agccattggt actgatactt taatatagta gagaagagat ataaattagg acgggtacaa 660caagaccaca cgtactaaca tttttttttg tcacaggctg ctctaataca tatctctatg 720ataagcgagc tagggatgct agcgtgtcca tttgattcct atataaatct ccaattatag 780ctgtagcaat taatttaata aacacccaac aatagatcaa atctcatagc aaatcataat 840catgaatgct ccaaaatcag ctagctggct ctcccttatc ttcgtttttc cttcttctcc 900tgcaacgaaa agaaaaaaaa agaaaagaaa agaaaacggc cgcttgtggt actaactccc 960aactacgcac ctaccgcgcg cataactctt ggccgcctgc cctcatcacc tccgcgtcgc 1020cgtcgactca tccttatcct ccccatcacg ctcaccccgc gcccgcaccg cgccatccgt 1080actttcccgg ccgccccacc gctggccgcc ccgacgtgtc gcgccgccac cggaaggtcc 1140cgggccgtcg ggcgggcaga gcgcctgcag cggtggaccc acgccacgct gacgcgggcg 1200cgcgtccgtc caagaaacct gacgtaagca gtgacagaat tggcgccgcc tctcggcgtc 1260cacgtgtcgt ggtcaacctg tcagagtggg gctccgtgtg tgcgctaccg caggggcccg 1320gcgcacgggc cacacgtgtc gcggtcgacc gcggctataa atgcccggct ccgcactcgg 1380aacaagtttc aagctctcct cccctcttcc taccattagc agtagccaca gccagaacac 1440cagcagacag cagcatcagc agggaggaac agcggcccac cgtcttcggt acgcgctcac 1500tccgccctct gcctttgtta ctgccacgtt tctctgaatg ctctcttgtg tggtgattgc 1560tgagagtggt ttagctggat ctagaattac actctgaaat cgtgttctgc ctgtgctgat 1620tacttgccgt cctttgtagc agcaaaatat agggacatgg tagtacgaaa cgaagataga 1680acctacacag caatacgaga aatgtgtaat ttggtgctta gcggtattta tttaagcaca 1740tgttggtgtt atagggcact tggattcaga agtttgctgt taatttaggc acaggcttca 1800tactacatgg gtcaatagta tagggattca tattataggc gatactataa taatttgttc 1860gtctgcagag cttattattt gccaaaatta gatattccta ttctgttttt gtttgtgtgc 1920tgttaaattg ttaacgcctg aaggaataaa tataaatgac gaaattttga tgtttatctc 1980tgctccttta ttgtgaccat aagtcaagat cagatgcact tgttttaaat attgttgtct 2040gaagaaataa gtactgacag tattttgatg cattgatctg cttgtttgtt gtaacaaaat 2100ttaaaaataa agagtttcct ttttgttgct ctccttacct cctgatggta tctagtatct 2160accaactgac actatattgc ttctctttac atacgtatct tgctcgatgc cttctcccta 2220gtgttgacca gtgttactca catagtcttt gctcatttca ttgtaatgca gataccaagc 2280gg 22821011546DNAArtificial Sequencemeristem and cob enhanced promoter 101cctagcgtcc cctgcccaaa agtgggctca acaagcctaa atacatataa tttataccac 60gtgcaacaca tttattcatc catatcacat gtcatgcaag gcataagcat catgttaact 120tagttatact gacatacatt tatgagttga gatgtccagg atgtgagcgc atgagcccat 180tgtccattca ggaccaagac aggctactaa gcactttcta cataacttgt atgtgctaac 240tatagcatgc ttatatggct ctctccaaag ttcaaagcta gctcaaatct tttgatttaa 300taaaacttaa atttgtttga tttcagataa actgataatt tttataatat ttagagtgag 360ttgaaaacag aaactggccg caaatccacc tcaagccttt tgatttgacc taaaaaaaag 420aagcccccac aaacaccact ccacactagt gcactgtctc tctccaaagg cagctgcatt 480ggcctccagc cttttcccta ctgtgccgcg cgccctccct tctctctaat aatagcatag 540ggagagaagg catactccga ggcatccttc tcctttccct ctccttcccc aaaccctttt 600cctctttccc tcgccccaag aacttcatct catctccagg cgcccctttt gcgcttgcgc 660aggaggagct cacggggaca gtgggcggag agctcgatcg ctgcaccact acttcactgg 720aggtccgccc actcccgggc ccaccgtctt cggtacgcgc tcactccgcc ctctgccttt 780gttactgcca cgtttctctg aatgctctct tgtgtggtga ttgctgagag tggtttagct 840ggatctagaa ttacactctg aaatcgtgtt ctgcctgtgc tgattacttg ccgtcctttg 900tagcagcaaa atatagggac atggtagtac gaaacgaaga tagaacctac acagcaatac 960gagaaatgtg taatttggtg cttagcggta tttatttaag cacatgttgg tgttataggg 1020cacttggatt cagaagtttg ctgttaattt aggcacaggc ttcatactac atgggtcaat 1080agtataggga ttcatattat aggcgatact ataataattt gttcgtctgc agagcttatt 1140atttgccaaa attagatatt cctattctgt ttttgtttgt gtgctgttaa attgttaacg 1200cctgaaggaa taaatataaa tgacgaaatt ttgatgttta tctctgctcc tttattgtga 1260ccataagtca agatcagatg cacttgtttt aaatattgtt gtctgaagaa ataagtactg 1320acagtatttt gatgcattga tctgcttgtt tgttgtaaca aaatttaaaa ataaagagtt 1380tcctttttgt tgctctcctt acctcctgat ggtatctagt atctaccaac tgacactata 1440ttgcttctct ttacatacgt atcttgctcg atgccttctc cctagtgttg accagtgtta 1500ctcacatagt ctttgctcat ttcattgtaa tgcagatacc aagcgg 1546

Claims

1. A recombinant DNA construct comprising: a) a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29; or b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92.

2. The recombinant DNA construct of claim 1 comprising a promoter functional in a plant cell and operably linked to the polynucleotide sequence.

3. A vector or plasmid comprising the recombinant DNA construct of claim 1.

4. A plant or seed comprising the recombinant DNA construct of claim 1.

5. The plant or seed of claim 4, wherein the plant or seed is a field crop plant or seed.

6. The plant of claim 4, wherein the field crop is selected from the group consisting of corn, soybean, cotton, canola, rice, barley, oat, wheat, turf grass, alfalfa, sugar beet, sunflower, quinoa and sugarcane.

7. The plant of claim 4, wherein the plant has an altered phenotype or an enhanced trait as compared to a control plant.

8. The plant of claim 7, wherein the enhanced trait or altered phenotype is selected from the group consisting of: decreased days from planting to maturity, increased stalk size, increased number of leaves, increased plant height growth rate in vegetative stage, increased ear size, increased ear dry weight per plant, increased number of kernels per ear, increased weight per kernel, increased number of kernels per plant, decreased ear void, extended grain fill period, reduced plant height, increased number of root branches, increased total root length, increased yield, increased nitrogen use efficiency, altered plant height, altered biomass, altered canopy area, altered anthocyanin content, altered chlorophyll content, altered water applied, altered water content, altered water use efficiency and increased water use efficiency as compared to a control plant.

9. (canceled)

10. A propagule comprising the recombinant DNA construct of claim 1, wherein the propagule is selected from the group consisting of cells, pollen, ovule, flower, embryo, leaf, root, stem, shoot, meristem, grain and seed.

11. A method for altering a phenotype, enhancing a trait, increasing yield, increasing nitrogen use efficiency, or increasing water use efficiency in a plant comprising producing a transgenic plant comprising a recombinant DNA construct according to claim 1.

12. The method of claim 11, wherein the recombinant DNA construct comprises a promoter functional in a plant cell and operably linked to the polynucleotide sequence.

13. The method of claim 11, wherein the plant is produced by: a) transforming a plant cell or tissue with the recombinant DNA construct, and regenerating or developing the transgenic plant from the plant cell or tissue comprising the recombinant DNA construct, or b) site-directed integration of the recombinant DNA construct into the genome of a plant cell or tissue using a donor template comprising the recombinant DNA construct, and regenerating or developing the transgenic plant from the plant cell or tissue comprising the recombinant DNA construct.

14. The method of claim 11, comprising: producing a progeny plant comprising the recombinant DNA construct by crossing the transgenic plant with: a) itself; b) a second plant from the same plant line; c) a wild type plant; or d) a second plant from a different plant line, to produce a seed, growing the seed to produce a progeny plant; and selecting a progeny plant with increased yield, increased nitrogen use efficiency, or increased water use efficiency as compared to a control plant.

15. (canceled)

16. A plant produced by the method of claim 11.

17. A recombinant DNA molecule for use as a donor template in site-directed integration, wherein the recombinant DNA molecule comprises an insertion sequence comprising: a) a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29; or b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92.

18. The recombinant DNA molecule of claim 17, wherein a) the insertion sequence further comprises a promoter functional in a plant cell and operably linked to the polynucleotide sequence, b) the recombinant DNA molecule comprises at least one homology arm flanking the insertion sequence, c) the recombinant DNA molecule comprises at least one cassette encoding site-specific nuclease, wherein the site specific nuclease is selected from the group comprising zinc-finger nuclease, an engineered or native meganuclease, a TALE-endonuclease, or an RNA-guided endonuclease, or d) the recombinant DNA molecule comprises at least one cassette encoding one or more guide RNAs.

19-21. (canceled)

22. A recombinant DNA molecule for use as a donor template in site-directed integration, wherein the recombinant DNA molecule comprises an insertion sequence for modulation of expression of an endogenous gene, wherein the endogenous gene comprises: a) a polynucleotide sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 1-29, or a portion thereof; or b) a polynucleotide sequence that encodes a polypeptide having an amino acid sequence with at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, or 100% identity to a sequence selected from the group consisting of SEQ ID NOs: 30-92.

23. The recombinant DNA construct of claim 22, wherein: a) the insertion sequence comprises a promoter, an enhancer, an intron, or a terminator region b) the recombinant DNA molecule further comprises at least one cassette encoding site-specific nuclease, wherein the site specific nuclease is selected from the group comprising zinc-finger nuclease, an engineered or native meganuclease, a TALE-endonuclease, or an RNA-guided endonuclease, or c) the recombinant DNA molecule further comprises at least one cassette encoding one or more guide RNAs.

24-25. (canceled)

26. A method for altering a phenotype, enhancing a trait, increasing yield, increasing nitrogen use efficiency, or increasing water use efficiency in a plant comprising: a) modifying the genome of a plant cell by: i) identifying an endogenous gene of the plant corresponding to a gene selected from the list of genes in Tables 1 and 12, and their homologs, and ii) modifying a sequence of the endogenous gene in the plant cell via site-directed integration to modify the expression level of the endogenous gene; and b) regenerating or developing a plant from the plant cell.