METHODS AND MEANS OF INCREASING THE WATER USE EFFICIENCY OF PLANTS

Abstract

The invention relates to methods of producing a desired phenotype in a plant by manipulation of gene expression within the plant. The method relates to means which inhibit the level of PK220 gene expression or activity, wherein a desired phenotype such as increased water use efficiency relative to a wild type control plant. The invention also relates to nucleic acid sequences and constructs useful such methods and methods of generating and isolating plants having decreased PK220 expression or activity.

Description

REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. patent application Ser. No. 16/019,077, filed on Jun. 26, 2018, which is a continuation of U.S. patent application Ser. No. 15/266,276, filed on Sep. 15, 2016, now U.S. Pat. No. 10,036,035, which is a continuation of U.S. patent application Ser. No. 12/483,660, filed on Jun. 12, 2009, now U.S. Pat. No. 9,453,238, which claims the benefit of U.S. Ser. No. 61/132,067, filed Jun. 13, 2008, the contents of each of which are incorporated herein by reference in their entirety.

INCORPORATION-BY-REFERENCE OF SEQUENCE LISTING

[0002] The contents of the text file named "PREP-017_C03US SEQ LISTING.txt", which was created on Nov. 7, 2019 and is 225 KB in size, are hereby incorporated by reference in their entireties.

FIELD OF THE INVENTION

[0003] The invention is in the field of plant molecular biology and relates to transgenic plants having novel phenotypes, methods of producing such plants and polynucleotides and polypeptides useful in such methods. More specifically, the invention relates to inhibition of a protein kinase and transgenic plants having inhibited protein kinase activity.

BACKGROUND OF THE INVENTION

[0004] Water is essential for plant survival, growth and reproduction. Assimilation of carbon dioxide by photosynthesis is directly linked to water loss through the stomata. Crop productivity which is closely linked to biomass production is dependent on plant water use efficiency (WUE) especially in water limited conditions (Passioura 1994 and Sinclair 1994, in Physiology and Determination of Crop Yield). Water use efficiency over a period of plant's growth can be calculated as the ratio of biomass produced per unit of water transpired (Sinclair 1994). Instantaneous measurements of water use efficiency can also be obtained as the ratio of carbon dioxide assimilation to transpiration using gas exchange measurements (Farquhar and Sharkey 1994, in Physiology and Determination of Crop Yield). Since there is a close correlation between crop productivity and water use efficiency, many attempts have been made to study and understand this relationship and the genetic components involved. To maximize the productivity and yield of a crop, efforts have been made to try to improve the water use efficiency of plants (Condon et al., 2002, Araus et al., 2002, Davies et al., 2002). Higher water use efficiency can be achieved either by increasing the biomass production and carbon dioxide assimilation or by reducing the transpiration water loss. Reduced transpiration, especially under non-limiting water conditions can be associated with reduced growth rate and therefore reduced crop productivity. This poses a dilemma on how to improve crop productivity and yield under water limited conditions but also maintain it under irrigated or non-limited water conditions (Condon et al., 2002).

[0005] Improvements to water use efficiency, to date, have used plant breeding methods whereby high water use efficiency varieties were crossed with the more productive but lower water use efficiency varieties in hope of improvements in crop yield under water limited conditions (Condon et al., 2002, Araus et al., 2002). Quantitative trait loci (QTL) approaches to identifying the components of water use efficiency have been the most common methods historically used (Mian et al., 1996, Martin et al., 1989, Thumma et al., 2001, Price et al., 2002), and more recently attempts have been made to engineer improved plants by molecular genetic means.

[0006] The first gene associated with water use efficiency was ERECTA. The ERECTA gene was first identified as a gene functioning in inflorescence development and organ morphogenesis (Torii et al., 1996)). It was later found by QTL mapping to be a major contributor to transpiration efficiency, defined as water transpired per carbon dioxide assimilated, an opposite indicator to water use efficiency in Arabidopsis (Masle et al., 2005). ERECTA encodes a putative leucin-rich repeat receptor-like kinase (LRR-RLK). The regulatory mechanism of LRR-RLK is yet to be understood although it was suggested due to, at least in part, the effects on stomatal density, epidermal cell expansion, mesophyll cell proliferation and cell-cell contact. The normal transpiration efficiency was restored upon complementation using wild type ERECTA in mutant exacta. However, it is not known whether overexpression of ERECTA in transgenic Arabidopsis will result in reduced transpiration efficiency or enhanced water use efficiency. It is the only report showing a plant receptor-like kinase to be involved in transpiration efficiency or water use efficiency.

[0007] Another Arabidopsis gene implicated in water use efficiency is the HARDY gene, found through the phenotypic screening of an activation tagged mutant collection (Karaba et al., 2007). Overexpression of HARDY in rice resulted in improved water use efficiency by enhancing photosynthetic assimilation and reducing transpiration. The transgenic rice with increased expression of HARDY exhibited increased shoot biomass under optimal water conditions and increased root biomass under water limited conditions. Overexpression of HARDY in Arabidopsis resulted in thicker leaves with more mesophyll cells and in rice increased leaf biomass and bundle sheet cells. These modifications contributed to enhanced photosynthetic activity and efficiency (Karaba et al., 2007).

[0008] Protein kinases are a large family of enzymes that modify proteins by addition of phosphate groups (phosphorylation). Protein kinases constitute about 2% of all eukaryotic genes, many of which mediate the response of eukaryotic cells to external stimuli. All single subunit protein kinases contain a common catalytic domain near the carboxyl terminus while the amino terminus plays a regulatory role.

[0009] Plant receptor-like kinases are serine/threonine protein kinases with a predicted signal peptide at the amino terminus, a single transmembrane region and a cytoplasmic kinase domain. There are more than 610 RLKs potentially encoded in Arabidopsis (Shiu and Bleecker 2001). Receptor-like kinases are often part of a signaling cascade. They interpret extracellular signals, through ligand binding, and phosphorylate targets in a signaling cascade which in turn affect downstream cell processes, such as gene expression (Hardie 1999).

[0010] Identification of genes that can be manipulated to provide beneficial characteristics is highly desirable. So too are means and methods of utilizing the identified genes to effect the desirable characteristics. The receptor-like kinase identified as At2g25220 in the TAIR database is one serine/threonine kinase, and a member of the large gene family of receptor-like kinases with over 600 members in Arabidopsis (Shiu et al., 2001). However, except for annotation of the sequence as a kinase no function or role for the At2g25220 gene has been disclosed. In the present invention a high water use efficiency gene (HWE) has been identified that when its expression or activity is inhibited results in beneficial phenotypes, such as, enhancement of plant biomass accumulation relative to the water used. This occurs under both water limited and non-limited conditions and ensures better growth and therefore greater productivity of the plants.

SUMMARY OF THE INVENTION

[0011] This invention is bases upon the discovery of a mutation in the PK220 gene that results in a plant with an altered phenotype such for example, increased water use efficiency, increased drought tolerance, reduced sensitivity to cold temperature and reduced inhibition of seedling growth in low nitrogen conditions compared to plants without the mutation.

[0012] More specifically, the invention relates to the identification of a mutant plant that comprises a mutation in the PK220 gene also referred to herein as the HWE gene. The PK220 gene is a receptor-like protein kinase. Inhibition of the expression or activity of the PK220 gene in plants provides beneficial phenotypes such as improved water use efficiency in a plant. The improved water use efficiency phenotype results in plants having improved drought tolerance.

[0013] In one aspect the invention provides a method of producing a transgenic plant, by transforming a plant, a plant tissue culture, or a plant cell with a vector containing a nucleic acid construct that inhibits the expression or activity of a PK220 gene to obtain a plant, tissue culture or a plant cell with decreased PK220 expression or activity and growing the plant or regenerating a plant from the plant tissue culture or plant cell. wherein a plant having increased water use efficiency is produced.

[0014] Accordingly, the present invention provides a method of producing a plant having an improved property, wherein the method includes inhibiting the expression or activity of an endogenous PK220 gene, wherein a plant is produced having an advantageous phenotype or improved property. In a particular embodiment, the present invention provides a method for producing plants having increased water use efficiency, wherein the method includes include generation of transgenic plants and modification of plants genome using the methods described herein.

[0015] Water use efficiency refers to the ratio between the amounts of biomass produced per unit water transpired when measured gravimetrically and the ratio of photosynthetic rate to the rate of transpiration when measured using gas exchange quantification of a leaf or shoot. As used herein, the term "increased water use efficiency" refers to a plant water use efficiency that is 2, 4, 5, 6, 8, 10, 20 or more fold greater as compared to the water use efficiency of a corresponding wild-type plant. For example, a plant having increased water use efficiency as compared to a wild-type plant may have 5%, 10%, 15%, 20%, 25%, 30%, 40%, 50%, 60% 70%, 75% or greater water use efficiency than the corresponding wild-type plant.

[0016] The methods of the invention involve inhibiting or reduced the expression or activity of an endogenous gene, such as PK220, wherein a plant is produced having an advantageous phenotype or improved property, such as increased water use efficiency. In one aspect, the invention provides a method of producing a plant having increased water use efficiency relative to a wild-type plant, by introducing into a plant cell a nucleic acid construct that inhibits or reduces the expression or activity of PK220. For example, a plant having increased water use efficiency relative to a wild type plant is produced by a) providing a nucleic acid construct containing a promoter operably linked to a nucleic acid construct that inhibits PK220 activity; b) inserting the nucleic construct into a vector; c) transforming a plant, tissue culture, or a plant cell with the vector to obtain a plant, tissue culture or a plant cell with decreased PK220 activity; d) growing the plant or regenerating a plant from the tissue culture or plant cell, wherein a plant having increased water use efficiency relative to a wild type plant is produced. The construct includes a promoter such as a constitutive promoter, a tissue specific promoter or an inducible promoter. Preferably, the tissue specific promoter is a root promoter. A preferable inducible promoter is a drought inducible promoter.

[0017] The term "nucleic acid construct" refers to a full length gene sequence or portion thereof, wherein a portion is preferably at least 19, 20, 21, 22, 23, 24, 25, 30, 40, 50, 60, 70, 75, 80, 90, 100, or 150 nucleotides in length, or the compliment thereof. Alternatively it may be an oligonucleotide, single or double stranded and made up of DNA or RNA or a DNA-RNA duplex. In a particular embodiment, the nucleic acid construct contains the full length PK220 gene sequence, or a portion thereof, wherein the portion of the PK220 sequence is at least 19, 20, 21, 22, 23, 24, 25, 30, 40, 50, 60, 70, 75, 80, 90, 100, or 150 nucleotides in length, or its compliment.

[0018] Also provided by the invention is a transgenic plant having an advantageous phenotype or improved property such as increased water use efficiency, produced by the methods described herein.

[0019] In another aspect the invention provides a plant having a non-naturally occurring mutation in an PK220 gene, wherein the plant has decreased PK220 expression or activity and the plant has increased water use efficiency relative to a wild-type control. Decreased PK220 expression or activity refers to a 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60, or 75-fold reduction or greater, at the DNA, RNA or protein level of an PK220 gene as compared to wild-type PK220, or a 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60 or 75 fold reduction of PK220 activity as compared to wild-type PK220 activity. PK220 activity includes but is not limited kinase activity at serine and or threonine amino acid residues of substrate polypeptides, where it participates in phosphorylation reactions.

[0020] The invention further provides a transgenic seed produced by the transgenic plant(s) of the invention, wherein the seed produces plant having an advantageous phenotype or improved property such as for example, increased water use efficiency relative to a wild-type plant.

[0021] In another embodiment, the invention provides nucleic acids for expression of nucleic acids in a plant cell to produce a transgenic plant having an advantageous phenotype or improved property such as increased water use efficiency.

[0022] Exemplary sequences encoding a wild type PK220 gene or portion thereof that find use in aspects of the present invention are described in SEQ ID NO's: 1, 7, 9, 11, 12, 13, 24, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 84, 86, 88, 90, 92, 94, 96, 98, 100, 153, 161 and 193. Exemplary sequences encoding a mutated PK220 gene are described in SEQ ID NO's:3 and 5. Exemplary sequences that are useful for constructs to downregulate PK220 expression or activity are described in SEQ ID NO's: 12, 13, 147, 149, 153, 161, 168 and 174. The invention further provides compositions which contain the nucleic acids of the invention for expression in a plant cell to produce the transgenic plants described herein.

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

[0024] Other features and advantages of the invention will be apparent from and are encompassed by the following detailed description and claims.

DETAILED DESCRIPTION

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In the case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting.

[0026] For convenience, before further description of the present invention, certain terms employed in the specification, examples and claims are defined herein. These definitions should be read in light of the remainder of the disclosure and as understood by a person of ordinary skill in the art.

[0027] A "promoter sequence", or "promoter", means a nucleic acid sequence capable of inducing transcription of an operably linked gene sequence in a plant cell. Promoters include for example (but not limited to) constitutive promoters, tissue specific promoters such as a root promoter, an inducible promoters such as a drought inducible promoter or an endogenous promoters such as a promoter normally associated with a gene of interest, i.e. a PK220 gene

[0028] The term "expression cassette" means a vector construct wherein a gene or nucleic acid sequence is transcribed. Additionally, the expressed mRNA may be translated into a polypeptide.

[0029] The terms "expression" or "overexpression" are used interchangeably and mean the expression of a gene such that the transgene is expressed. The total level of expression in a cell may be elevated relative to a wild-type cell.

[0030] The term "non-naturally occurring mutation" refers to any method that introduces mutations into a plant or plant population. For example, chemical mutagenesis such as ethane methyl sulfonate or methanesulfonic acid ethyl ester, fast neutron mutagenesis, DNA insertional means such as a T-DNA insertion or site directed mutagenesis methods.

[0031] The term "drought stress" refers to a condition where plant growth or productivity is inhibited relative to a plant where water is not limiting. The term "water-stress" is used synonymously and interchangeably with the drought water stress.

[0032] The term "drought tolerance" refers to the ability of a plant to outperform a wildtype plant under drought stress conditions or water limited conditions or to use less water during grow and development relative to a wildtype plant.

[0033] The "term water use efficiency" is an expression of the ratio between the amounts of biomass produced per unit water transpired when measured gravimetrically and the ratio of photosynthetic rate to the rate of transpiration when measured using gas exchange quantification of a leaf or shoot.

[0034] The term "dry weight" means plant tissue that has been dried to remove the majority of the cellular water and is used synonymously and interchangeably with the term biomass.

[0035] The term "null" is defined as a segregated sibling of a transgenic line that has lost the inserted transgene and is therefore used a control line.

[0036] A number of various standard abbreviations have been used throughout the disclosure, such as g, gram; WT, wild-type; DW, dry weight; WUE, water use efficiency; d, day.

[0037] The term "hwe116" means a plant having a mutation in a PK220 gene.

[0038] The HWE gene is referred to as a PK220 gene sequence and a protein encoded by a PK220 gene is referred to as a PK220 polypeptide or protein. The terms HWE and PK220 are synonymous.

[0039] The term "PK220 nucleic acid" refers to at least a portion of a PK220 nucleic acid. Similarly the term "PK220 protein" or "PK220 polypeptide" refers to at least a portion thereof. A portion is of at least 21 nucleotides in length with respect to a nucleic acid and a portion of a protein or polypeptide is at least 7 amino acids. The term "AtPK220" refers to an Arabidopsis thaliana PK220 gene, the term "BnPK220" refers to a Brassica napus PK220 gene.

[0040] The invention is based in part on the discovery of plants having an improved agronomic property, for example, increased water use efficiency, increased drought tolerance, reduced sensitivity to cold temperature and reduced inhibition of seedling growth in low nitrogen conditions relative to a wild type control. The gene responsible for the beneficial phenotype has been determined and shown to be an inhibited PK220 gene.

[0041] Methods of producing a plant, including a mutant plant, a transgenic plant or genetically modified plant, having increased water use efficiency are disclosed herein. Specifically the invention identifies a PK220 gene that when expression or activity of the PK220 gene is inhibited, a plant having a beneficial phenotype is obtained.

Determining Homology Between Two or More Sequences

[0042] To determine the percent homology between two amino acid sequences or between two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in either of the sequences being compared for optimal alignment between the sequences). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are homologous at that position (i.e., as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity").

[0043] The nucleic acid sequence homology may be determined as the degree of identity between two sequences. The homology may be determined using computer programs known in the art, such as GAP software provided in the GCG program package. See, Needleman and Wunsch (1970). Using GCG GAP software with the following settings for nucleic acid sequence comparison: GAP creation penalty of 5.0 and GAP extension penalty of 0.3, the coding region of the analogous nucleic acid sequences referred to above exhibits a degree of identity preferably of at least 70%, 75%, 80%, 85%, 90%, 95%, 98%, or 99%, with the coding sequence portion of the DNA sequence shown in SEQ ID NO:1.

[0044] The term "sequence identity" refers to the degree to which two polynucleotide or polypeptide sequences are identical on a residue-by-residue basis over a particular region of comparison. The term "percentage of sequence identity" is calculated by comparing two optimally aligned sequences over that region of comparison, determining the number of positions at which the identical nucleic acid base (e.g., A, T, C, G, U, or I, in the case of nucleic acids) occurs in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the region of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of sequence identity. The term "substantial identity" as used herein denotes a characteristic of a polynucleotide sequence, wherein the polynucleotide comprises a sequence that has at least 80 percent sequence identity, preferably at least 85 percent identity and often 90 to 95 percent sequence identity, more usually at least 99 percent sequence identity as compared to a reference sequence over a comparison region. The term "percentage of positive residues" is calculated by comparing two optimally aligned sequences over that region of comparison, determining the number of positions at which the identical and conservative amino acid substitutions, as defined above, occur in both sequences to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the region of comparison (i.e., the window size), and multiplying the result by 100 to yield the percentage of positive residues.

Inhibition of Endogenous PK220 Expression and Activity

[0045] An aspect of the invention pertains to means and methods of inhibiting or reducing PK220 gene expression and activity, optionally, resulting in an inhibition or reduction of PK220 protein expression and activity. The term "PK220 expression or activity" embraces both these levels of inhibition or reduction. Decreased PK220 expression or activity refers to a 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60, or 75-fold reduction or greater, at the DNA, RNA or protein level of an PK220 gene as compared to wild-type PK220, or a 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60 or 75 fold reduction of PK220 protein activity as compared to wild-type PK220 activity. PK220 protein activity includes but is not limited kinase activity at serine and or threonine amino acid residues of substrate polypeptides, where it participates in phosphorylation reactions. Methods of measuring serine/threonine kinase activity are known to those in the art.

[0046] There are numerous methods known to those skilled in the art of achieving such inhibition that effect a variety of steps in a gene expression pathway, for example transcriptional regulation, post transcriptional and translational regulation. Such methods include, but are not limited to, antisense methods, RNAi constructs, including all hairpin constructs and RNAi constructs useful for inhibition by dsRNA-directed DNA methylation or inhibition by mRNA degradation or inhibition of translation, microRNA (miRNA), including artificial miRNA (amiRNA) (Schwab et al., 2006) technologies, mutagenesis and TILLING methods, in vivo site specific mutagenesis techniques and dominant/negative inhibition approaches.

[0047] A preferred method of gene inhibition involves RNA inhibition (RNAi) also known as hairpin constructs. A portion of the gene to inhibit is used and cloned in a sense and antisense direction having a spacer separating the sense and antisense portions. The size of the gene portions should be at least 20 nucleotides in length and the spacer may be a little as 13 nucleotides (Kennerdell and Carthew, 2000) in length and may be an intron sequence, a coding or non-coding sequence.

[0048] Antisense is a common approach wherein the target gene, or a portion thereof, is expressed in an antisense orientation resulting in inhibition of the endogenous gene expression and activity. The antisense portions need not be a full length gene nor be 100% identical. Provided that the antisense is at least about 70% or more identical to the endogenous target gene and of least 19, 20, 21, 22, 23, 24, 25, 30, 40, 50, 60, 70, 75, 80, 90, 100, or 150 nucleotides in length. Preferably, 50 nucleotides or greater in length the desired inhibition will be obtained.

[0049] Sequences encoding a wild type PK220 gene or portion thereof that are useful in preparing constructs for PK220 inhibition include for example, SEQ ID NO's: 1, 7, 9, 11, 12, 13, 24, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 84, 86, 88, 90, 92, 94, 96, 98, 100, 153, 161 and 193. Exemplary sequences that are useful for constructs to downregulate PK220 expression or activity are described in SEQ ID NO's: 12, 13, 147, 149, 153, 161, 168 and 174.

[0050] When using an antisense strategy of down-regulation, inhibition of endogenous gene activity can be selectively targeted to the gene or genes of choice by proper selection of a fragment or portion for antisense expression. Selection of a sequence that is present in the target gene sequence and not present in related genes (non-target gene) or is less than 70% conserved in the non-target sequences results in specificity of gene inhibition.

[0051] Alternatively, amiRNA inhibition can be used to inhibit gene expression and activity in a more specific manner than other RNAi methods. In contrast to siRNA that requires a perfect match between the small RNA and the target mRNA, amiRNA allows up to 5 mismatches with no more than 2 consecutive mismatches. The construction of amiRNA needs to meet certain criteria described in Schawab et al. (2006). This provides a method to down-regulate a target gene expression or activity using a gene portion comprising of at least a 21 nucleotide sequence of PK220.

[0052] Dominant/negative inhibition is analogous to competitive inhibition of biochemical reactions. Expression of a modified or mutant polypeptide that lacks full functionality competes with the wild type or endogenous polypeptide thereby reducing the total gene/protein activity. For example an expressed protein may bind to a protein complex or enzyme subunit to produce a non-functional complex. Alternatively the expressed protein may bind substrate but not have activity to perform the native function. Expression of sufficient levels of non active protein will reduce or inhibit the overall function.

[0053] Expression of PK220 genes that produce a PK220 protein that is deficient in activity can be used for dominant/negative down-regulation of gene activity. This is analogous to competitive inhibition. A PK220 polypeptide is produced that, for example, may associate with or bind to a target molecule but lacks endogenous activity. An example of such an inactive PK220 is the AtPK220 sequence isolated from the hwe116 mutant and disclosed as SEQ ID NO:3. A target molecule may be an interacting protein of a nucleic acid sequence. In this manner the endogenous PK220 protein is effectively diluted and downstream responses will be attenuated.

[0054] In vivo site specific mutagenesis is available whereby one can introduce a mutation into a cells genome to create a specific mutation. The method as essentially described in Dong et al. (2006) or US patent application publication number 20060162024 which refer to the methods of oligonucleotide-directed gene repair. Alternatively one may use chimeric RNA/DNA oligonucleotides essentially as described Beetham (1999). Accordingly, a premature stop codon may be generated in the cells' endogenous gene thereby producing a specific null mutant. Alternatively, the mutation may interfere with splicing of the initial transcript thereby creating a non-translatable mRNA or a mRNA that produces an altered polypeptide which does not possess endogenous activity. Preferable mutations that result loss or reduction of PK220 expression or activity include a C to T conversion at nucleotide position 874 when numbered in accordance with SEQ ID NOs: 1 or 3 or a nucleotide mutation that results in an amino acid change from a Leucine (L) codon (CTT) to a Phenylalanine (F) codon (TTT) at amino acid position 292 when numbered in accordance with SEQ ID NOs: 2 or 4.

[0055] TILLING is a method of isolating mutations in a known gene from an EMS-mutagenized population. The population is screened by methods essentially as described in (Greene et al., 2003).

[0056] Other strategies of gene inhibition will be apparent to the skilled worker including those not discussed here and those developed in the future.

Identification of AtPK220 Homologues

[0057] Homologues of Arabidopsis thaliana PK220 (AtPK220) were identified using database sequence search tools, such as the Basic Local Alignment Search Tool (BLAST) (Altschul et al., 1990 and Altschul et al., 1997). The tblastn or blastn sequence analysis programs were employed using the BLOSUM-62 scoring matrix (Henikoff and Henikoff, 1992). The output of a BLAST report provides a score that takes into account the alignment of similar or identical residues and any gaps needed in order to align the sequences. The scoring matrix assigns a score for aligning any possible pair of sequences. The P values reflect how many times one expects to see a score occur by chance. Higher scores are preferred and a low threshold P value threshold is preferred. These are the sequence identity criteria. The tblastn sequence analysis program was used to query a polypeptide sequence against six-way translations of sequences in a nucleotide database. Hits with a P value less than -25, preferably less than -70, and more preferably less than -100, were identified as homologous sequences (exemplary selected sequence criteria). The blastn sequence analysis program was used to query a nucleotide sequence against a nucleotide sequence database. In this case too, higher scores were preferred and a preferred threshold P value was less than -13, preferably less than -50, and more preferably less than -100.

[0058] A PK220 gene can be isolated via standard PCR amplification techniques. Use of primers to conserved regions of a PK220 gene and PCR amplification produces a fragment or full length copy of the desired gene. Template may be DNA, genomic or a cDNA library, or RNA or mRNA for use with reverse transcriptase PCR (RtPCR) techniques. Conserved regions can be identified using sequence comparison tools such as BLAST or CLUSTALW for example. Suitable primers have been used and described elsewhere in this application.

[0059] Alternatively, a fragment of a sequence from a PK220 gene is .sup.32P-radiolabeled by random priming (Sambrook et al., 1989) and used to screen a plant genomic library (the exemplary test polynucleotides). As an example, total plant DNA from Arabidopsis thaliana, Nicotiana tabacum, Lycopersicon pimpinellifolium, Prunus avium, Prunus cerasus, Cucumis sativus, or Oryza sativa are isolated according to Stockinger et al. (Stockinger et al., 1996). Approximately 2 to 10 .mu.g of each DNA sample are restriction digested, transferred to nylon membrane (Micron Separations, Westboro, Mass.) and hybridized. Hybridization conditions are: 42.degree. C. in 50% formamide, 5.times.SSC, 20 mM phosphate buffer 1.times.Denhardt's, 10% dextran sulfate, and 100 .mu.g/ml herring sperm DNA. Four low stringency washes at RT in 2.times.SSC, 0.05% sodium sarcosyl and 0.02% sodium pyrophosphate are performed prior to high stringency washes at 55.degree. C. in 0.2.times.SSC, 0.05% sodium sarcosyl and 0.01% sodium pyrophosphate. High stringency washes are performed until no counts are detected in the washout according to Walling et al. (Walling et al., 1988). Positive isolates are identified, purified and sequenced. Other methods are available for hybridization, for example the ExpressHyb hybridization solution available from Clonetech.

PK220 Recombinant Expression Vectors and Host Cells

[0060] Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding a PK220 protein, a PK220 gene or genomic sequence or portions thereof and analogs or homologs thereof. As used herein the term expression vector includes vectors which are designed to provide transcription of the nucleic acid sequence. Transcribed sequences may be designed to inhibit the endogenous expression or activity of an endogenous gene activity correlating to the transcribed sequence. Optionally, the transcribed nucleic acid need not be translated but rather inhibits the endogenous gene expression as in antisense or hairpin down-regulation methodology. Alternatively, the transcribed nucleic acid may be translated into a polypeptide or protein product. The polypeptide may be a non-full length, mutant or modified variant of the endogenous protein. As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial vectors having a bacterial origin of replication). Other vectors are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively-linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector. However, the invention is intended to include such other forms of expression vectors, such as viral vectors or plant transformation vectors, binary or otherwise, which serve equivalent functions.

[0061] The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, that is operatively-linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably-linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner that allows for expression of the nucleotide sequence (e.g., in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell).

[0062] The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements (e.g., polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel (1990). Regulatory sequences include those that direct constitutive expression of a nucleotide sequence in many types of host cell and those that direct expression of the nucleotide sequence only in certain host cells (e.g., tissue-specific regulatory sequences) or inducible promoters (e.g., induced in response to abiotic factors such as environmental conditions, heat, drought, nutrient status or physiological status of the cell or biotic such as pathogen responsive). Examples of suitable promoters include for example constitutive promoters, ABA inducible promoters, tissue specific promoters and abiotic or biotic inducible promoters. It will be appreciated by those skilled in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired as well as timing and location of expression, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein (e.g., PK220 proteins, mutant forms of PK220 proteins, fusion proteins, etc.).

[0063] The recombinant expression vectors of the invention can be designed for expression of PK220 genes, PK220 proteins, or portions thereof, in prokaryotic or eukaryotic cells. For example, PK220 genes or PK220 proteins can be expressed in bacterial cells such as Escherichia coli, insect cells (using baculovirus expression vectors)) yeast cells, plant cells or mammalian cells. Suitable host cells are discussed further in Goeddel (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

[0064] In one embodiment, a nucleic acid of the invention is expressed in plants cells using a plant expression vector. Examples of plant expression vectors systems include tumor inducing (Ti) plasmid or portion thereof found in Agrobacterium, cauliflower mosaic virus (CaMV) DNA and vectors such as pBI121.

[0065] For expression in plants, the recombinant expression cassette will contain in addition to the PK220 nucleic acids, a promoter region that functions in a plant cell, a transcription initiation site (if the coding sequence to transcribed lacks one), and optionally a transcription termination/polyadenylation sequence. The termination/polyadenylation region may be obtained from the same gene as the promoter sequence or may be obtained from different genes. Unique restriction enzyme sites at the 5' and 3' ends of the cassette are typically included to allow for easy insertion into a pre-existing vector.

[0066] Examples of suitable promoters include promoters from plant viruses such as the 35S promoter from cauliflower mosaic virus (CaMV) (Odell et al., 1985), promoters from genes such as rice actin (McElroy et al., 1990), ubiquitin (Christensen et al., 1992; pEMU (Last et al., 1991), MAS (Velten et al., 1984), maize H3 histone (Lepetit et al., 1992); and Atanassvoa et al., 1992), the 5'- or 3'-promoter derived from T-DNA of Agrobacterium tumefaciens, the Smas promoter, the cinnamyl alcohol dehydrogenase promoter (U.S. Pat. No. 5,683,439), the Nos promoter, the rubisco promoter, the GRP1-8 promoter, ALS promoter, (WO 96/30530), a synthetic promoter, such as Rsyn7, SCP and UCP promoters, ribulose-1,3-diphosphate carboxylase, fruit-specific promoters, heat shock promoters, seed-specific promoters and other transcription initiation regions from various plant genes, for example, including the various opine initiation regions, such as for example, octopine, mannopine, and nopaline. In some cases a promoter associated with the gene of interest (e.g. PK220) may be used to express a construct targeting the gene of interest, for example the native AtPK220 promoter (P.sub.PK). Additional regulatory elements that may be connected to a PK220 encoding nucleic acid sequence for expression in plant cells include terminators, polyadenylation sequences, and nucleic acid sequences encoding signal peptides that permit localization within a plant cell or secretion of the protein from the cell. Such regulatory elements and methods for adding or exchanging these elements with the regulatory elements of PK220 gene are known and include, but are not limited to, 3' termination and/or polyadenylation regions such as those of the Agrobacterium tumefaciens nopaline synthase (nos) gene (Bevan et al., 1983); the potato proteinase inhibitor II (PINII) gene (Keil et al., 1986) and hereby incorporated by reference); and An et al. (1989); and the CaMV 19S gene (Mogen et al., 1990).

[0067] Plant signal sequences, including, but not limited to, signal-peptide encoding DNA/RNA sequences which target proteins to the extracellular matrix of the plant cell (Dratewka-Kos et al., 1989) and the Nicotiana plumbaginifolia extension gene (De Loose et al., 1991), or signal peptides which target proteins to the vacuole like the sweet potato sporamin gene (Matsuoka et al., 1991) and the barley lectin gene (Wilkins et al., 1990), or signals which cause proteins to be secreted such as that of PRIb (Lund et al., 1992), or those which target proteins to the plastids such as that of rapeseed enoyl-ACP reductase (Verwoert et al., 1994) are useful in the invention.

[0068] In another embodiment, the recombinant expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type (e.g., tissue-specific regulatory elements are used to express the nucleic acid). Tissue-specific regulatory elements are known in the art. For example, the promoter associated with a coding sequence identified in the TAIR data base as At2g44790 (P.sub.4790) is a root specific promoter. Especially useful in connection with the nucleic acids of the present invention are expression systems which are operable in plants. These include systems which are under control of a tissue-specific promoter, as well as those which involve promoters that are operable in all plant tissues.

[0069] Organ-specific promoters are also well known. For example, the chalcone synthase-A gene (van der Meer et al., 1990) or the dihydroflavonol-4-reductase (dfr) promoter (Elomaa et al., 1998) direct expression in specific floral tissues. Also available are the patatin class I promoter is transcriptionally activated only in the potato tuber and can be used to target gene expression in the tuber (Bevan, 1986). Another potato-specific promoter is the granule-bound starch synthase (GBSS) promoter (Visser et al., 1991).

[0070] Other organ-specific promoters appropriate for a desired target organ can be isolated using known procedures. These control sequences are generally associated with genes uniquely expressed in the desired organ. In a typical higher plant, each organ has thousands of mRNAs that are absent from other organ systems (reviewed in Goldberg, 1986).

[0071] The resulting expression system or cassette is ligated into or otherwise constructed to be included in a recombinant vector which is appropriate for plant transformation. The vector may also contain a selectable marker gene by which transformed plant cells can be identified in culture. The marker gene may encode antibiotic resistance. These markers include resistance to G418, hygromycin, bleomycin, kanamycin, and gentamicin. Alternatively the marker gene may encode a herbicide tolerance gene that provides tolerance to glufosinate or glyphosate type herbicides. After transforming the plant cells, those cells having the vector will be identified by their ability to grow on a medium containing the particular antibiotic or herbicide. Replication sequences, of bacterial or viral origin, are generally also included to allow the vector to be cloned in a bacterial or phage host, preferably a broad host range prokaryotic origin of replication is included. A selectable marker for bacteria should also be included to allow selection of bacterial cells bearing the desired construct. Suitable prokaryotic selectable markers also include resistance to antibiotics such as kanamycin or tetracycline.

[0072] Other DNA sequences encoding additional functions may also be present in the vector, as is known in the art. For instance, in the case of Agrobacterium transformations, T-DNA sequences will also be included for subsequent transfer to plant chromosomes.

[0073] Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but also to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid (e.g., DNA) into a host cell.

[0074] A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce (i.e., express) a polypeptide of the invention encoded in an open reading frame of a polynucleotide of the invention. Accordingly, the invention further provides methods for producing a polypeptide using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding a polypeptide of the invention has been introduced) in a suitable medium such that the polypeptide is produced. In another embodiment, the method further comprises isolating the polypeptide from the medium or the host cell.

[0075] A number of cell types may act as suitable host cell for expression of a polypeptide encoded by an open reading frame in a polynucleotide of the invention. Plant host cells include, for example, plant cells that could function as suitable hosts for the expression of a polynucleotide of the invention include epidermal cells, mesophyll and other ground tissues, and vascular tissues in leaves, stems, floral organs, and roots from a variety of plant species, such as Arabidopsis thaliana, Nicotiana tabacum, Brassica napus, Zea mays, Oryza sativa, Gossypium hirsutum and Glycine max.

[0076] Expression of PK220 nucleic acids encoding a PK220 protein that is not fully functional can be useful in a dominant/negative inhibition method. A PK220 variant polypeptide, or portion thereof, is expressed in a plant such that it has partial functionality. The variant polypeptide may for example have the ability to bind other molecules but does not permit proper activity of the complex, resulting in overall inhibition of PK220 activity.

Transformed Plants Cells and Transgenic Plants

[0077] The invention includes a protoplast, plants cell, plant tissue and plant (e.g., monocot or dicot) transformed with a PK220 nucleic acid, a vector containing a PK220 nucleic acid or an expression vector containing a PK220 nucleic acid. As used herein, "plant" is meant to include not only a whole plant but also a portion thereof (i.e., cells, and tissues, including for example, leaves, stems, shoots, roots, flowers, fruits and seeds).

[0078] The plant can be any plant type including, for example, species from the genera Arabidopsis, Brassica, Oryza, Zea, Sorghum, Brachypodium, Miscanthus, Gossypium, Triticum, Glycine, Pisum, Phaseolus, Lycopersicon, Trifolium, Cannabis, Cucurbita, Rosa, Vitis, Juglans, Fragaria, Lotus, Medicago, Onobrychis, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Ciahorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Heterocallis, Nemesis, Pelargonium, Panieum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Brow aalia, Lolium, Avena, Hordeum, Secale, Picea, Caco, and Populus.

[0079] The invention also includes cells, tissues, including for example, leaves, stems, shoots, roots, flowers, fruits and seeds and the progeny derived from the transformed plant.

[0080] Numerous methods for introducing foreign genes into plants are known and can be used to insert a gene into a plant host, including biological and physical plant transformation protocols (See, for example, Miki et al., (1993) "Procedure for Introducing Foreign DNA into Plants", In: Methods in Plant Molecular Biology and Biotechnology, Glick and Thompson, eds., CRC Press, Inc., Boca Raton, pages 67-88; and Andrew Bent in, Clough S J and Bent AF, (1998) "Floral dipping: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana"). The methods chosen vary with the host plant, and include chemical transfection methods such as calcium phosphate, polyethylene glycol (PEG) transformation, microorganism-mediated gene transfer such as Agrobacterium (Horsch et al., 1985), electroporation, protoplast transformation, micro-injection, flower dipping and biolistic bombardment.

Agrobacterium-Mediated Transformation

[0081] The most widely utilized method for introducing an expression vector into plants is based on the natural transformation system of Agrobacterium tumefaciens and A. rhizogenes which are plant pathogenic bacteria which genetically transform plant cells. The Ti and Ri plasmids of A. tumefaciens and A. rhizogenes, respectfully, carry genes responsible for genetic transformation of plants (See, for example, Kado, 1991). Descriptions of the Agrobacterium vector systems and methods for Agrobacterium-mediated gene transfer are provided in Gruber et al. (1993). and Moloney et al., (1989).

[0082] Transgenic Arabidopsis plants can be produced easily by the method of dipping flowering plants into an Agrobacterium culture, based on the method of Andrew Bent in, Clough S J and Bent A F, 1998. Floral dipping: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Wild type plants are grown until the plant has both developing flowers and open flowers. The plants are inverted for 1 minute into a solution of Agrobacterium culture carrying the appropriate gene construct. Plants are then left horizontal in a tray and kept covered for two days to maintain humidity and then righted and bagged to continue growth and seed development. Mature seed is bulk harvested.

Direct Gene Transfer

[0083] A generally applicable method of plant transformation is microprojectile-mediated transformation, where DNA is carried on the surface of microprojectiles measuring about 1 to 4 .mu.m. The expression vector is introduced into plant tissues with a biolistic device that accelerates the microprojectiles to speeds of 300 to 600 m/s which is sufficient to penetrate the plant cell walls and membranes. (Sanford et al., 1993; Klein et al., 1992).

[0084] Plant transformation can also be achieved by the Aerosol Beam Injector (ABI) method described in U.S. Pat. Nos. 5,240,842, 6,809,232. Aerosol beam technology is used to accelerate wet or dry particles to speeds enabling the particles to penetrate living cells. Aerosol beam technology employs the jet expansion of an inert gas as it passes from a region of higher gas pressure to a region of lower gas pressure through a small orifice. The expanding gas accelerates aerosol droplets, containing nucleic acid molecules to be introduced into a cell or tissue. The accelerated particles are positioned to impact a preferred target, for example a plant cell. The particles are constructed as droplets of a sufficiently small size so that the cell survives the penetration. The transformed cell or tissue is grown to produce a plant by standard techniques known to those in the applicable art.

Regeneration of Transformants

[0085] The development or regeneration of plants from either single plant protoplasts or various explants is well known in the art (Weissbach and Weissbach, 1988). This regeneration and growth process typically includes the steps of selection of transformed cells, culturing those individualized cells through the usual stages of embryonic development through the rooted plantlet stage. Transgenic embryos and seeds are similarly regenerated. The resulting transgenic rooted shoots are thereafter planted in an appropriate plant growth medium such as soil.

[0086] The development or regeneration of plants containing the foreign, exogenous gene that encodes a polypeptide of interest introduced by Agrobacterium from leaf explants can be achieved by methods well known in the art such as described (Horsch et al., 1985). In this procedure, transformants are cultured in the presence of a selection agent and in a medium that induces the regeneration of shoots in the plant strain being transformed as described (Fraley et al., 1983). In particular, U.S. Pat. No. 5,349,124 (specification incorporated herein by reference) details the creation of genetically transformed lettuce cells and plants resulting therefrom which express hybrid crystal proteins conferring insecticidal activity against Lepidopteran larvae to such plants.

[0087] This procedure typically produces shoots within two to four months and those shoots are then transferred to an appropriate root-inducing medium containing the selective agent and an antibiotic to prevent bacterial growth. Shoots that rooted in the presence of the selective agent to form plantlets are then transplanted to soil or other media to allow the production of roots. These procedures vary depending upon the particular plant strain employed, such variations being well known in the art.

[0088] Preferably, the regenerated plants are self-pollinated to provide homozygous transgenic plants, or pollen obtained from the regenerated plants is crossed to seed-grown plants of agronomically important, preferably inbred lines. Conversely, pollen from plants of those important lines is used to pollinate regenerated plants. A transgenic plant of the present invention containing a desired polypeptide is cultivated using methods well known to one skilled in the art.

[0089] A preferred transgenic plant is an independent segregate and can transmit the PK220 gene construct to its progeny. A more preferred transgenic plant is homozygous for the gene construct, and transmits that gene construct to all offspring on sexual mating. Seed from a transgenic plant may be grown in the field or greenhouse, and resulting sexually mature transgenic plants are self-pollinated to generate true breeding plants. The progeny from these plants become true breeding lines that are evaluated for decreased expression of the PK220 gene.

Method of Producing Transgenic Plants

[0090] Also included in the invention are methods of producing a transgenic plant having increased water use efficiency, reduced sensitivity to cold temperature and reduced inhibition of seedling growth in low nitrogen conditions, relative to a wild type plant. The method includes introducing into one or more plant cells a compound that inhibits or reduces PK220 expression or activity in the plant to generate a transgenic plant cell and regenerating a transgenic plant from the transgenic cell. The compound can be, e.g., (i) a PK220 polypeptide; (ii) a PK220 nucleic acid, analog, homologue, orthologue, portion, variant or complement thereof; (iii) a nucleic acid that decreases expression of a PK220 nucleic acid. A nucleic acid that decreases expression of a PK220 nucleic acid may include promoters or enhancer elements. The PK220 nucleic acid can be either endogenous or exogenous, for example an Arabidoposis PK220 nucleic acid may be introduced into a Brassica or corn species. Preferably, the compound is a PK220 nucleic acid sequence endogenous to the species being transformed. Alternatively, the compound is a PK220 nucleic acid sequence exogenous to the species being transformed and having at least 70%, 75%, 80%, 85%, 90% or greater homology to the endogenous target sequence.

[0091] In various aspects the transgenic plant has an altered phenotype as compared to a wild type plant (i.e., untransformed). By altered phenotype is meant that the plant has a one or more characteristic that is different from the wild type plant. For example, when the transgenic plant has been contacted with a compound that decreases the expression or activity of a PK220 nucleic acid, the plant has a phenotype such as increased water use efficiency, reduced sensitivity to cold temperature and reduced inhibition of seedling growth in low nitrogen conditions, relative to a wild type plant.

[0092] The plant can be any plant type including, for example, species from the genera Arabidopsis, Brassica, Oryza, Zea, Sorghum, Brachypodium, Miscanthus, Gossypium, Triticum, Glycine, Pisum, Phaseolus, Lycopersicon, Trifolium, Cannabis, Cucurbita, Rosa, Vitis, Juglans, Fragaria, Lotus, Medicago, Onobrychis, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Ciahorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Heterocallis, Nemesis, Pelargonium, Panieum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Brow aalia, Lolium, Avena, Hordeum, Secale, Picea, Caco, and Populus.

Examples

Identification of High Water Use Efficiency Mutant Hwe116

[0093] An Arabidopsis EMS mutant (Columbia background) was identified initially as having drought tolerant properties. The mutant was tested for water use efficiency under optimal and drought conditions. The result showed that the drought tolerant nature of this mutant is due to its higher water use efficiency under both water stressed and optimal water conditions. Thus, this mutant is named hwe116.

Map Based Cloning of Hwe116

[0094] A F2 population was generated by crossing the hwe116 mutant to the Landsberg erecta (Ler) ecotype of Arabidopsis thaliana and the resulting population was used for map-based cloning by assaying for drought tolerance and subsequently confirming the presence of the higher water use efficiency trait in the mutant. The water-loss per unit dry weight of the F2 plants was measured over a 5-day drought treatment and the data was normalized for QTL analysis relative to the hwe116 mutant and the two wild type ecotypes, Landsberg erecta and Columbia. Leaf tissues were collected from all F2 and control plants used in the phenotyping experiments for genotyping. QTL analysis was conducted using MAPMAKER 3.0 and WinQTLCart 2.5. To further specify the mutations within the QTL peak, celery endonuclease I (CEL I) was used.

Mutation Detection Using CEL I Nuclease

[0095] Celery endonuclease I (CEL I), cleaves DNA with high specificity at sites of base-pair substitution that creates a mismatch between wild type and mutant alleles and has been reportedly used for detecting mutations in EMS mutants (Yang et al., 2000; Oleykowski et al., 1998).

[0096] DNA fragments of about 5 kb were amplified by optimized PCR using hwe116 or parent Columbia genomic DNA as template. Equal amounts of the amplified products were mixed together and then subjected to a cycle of denaturing and annealing to form heteroduplex DNA. Incubation with CEL I at 42.degree. C. for 20 minutes cleaves the heteroduplex DNA at points of mutation, and DNA fragments were visualized by 1% agarose gel electrophoresis and ethidium bromide staining.

[0097] Using this method a 5 kb PCR product was amplified using primers SEQ ID NO:102 and SEQ ID NO:104, and templates: hwe116, and the control Columbia type. The heteroduplexes formed PCR products resulted in smaller fragments (1.4 and 3.6 kb) after CEL I digestion. Overlapping sub-fragments (about 3 kb) were amplified using primers SEQ ID NO:104 and SEQ ID NO:105 to more narrowly define the mutation location. The sub-fragment was sequenced and a C nucleotide was found to have been mutated to T nucleotide in hwe116.

[0098] The mutation of interest was identified as a C to T conversion at nucleotide position 874 of SEQ ID NO's:1 and 3 that resulted in an amino acid change from a Leucine (L) codon (CTT) to a Phenylalanine (F) codon (TTT) at amino acid position 292. The gene harboring the mutation was identified as a Serine/Threonine protein kinase (Ser/Thr PK). The wild type gene was identified as being identical to Genbank Accession Number At2g25220. This Ser/Thr protein kinase is referred to as AtPK220 herein, and the mutated form identified in hwe116 is referred to as AtPK220L292F.

Transcriptional Evaluation

[0099] Northern analysis and RT-PCR indicate that the expression level and transcript size of the AtPK220 gene in hwe116 is unchanged relative to the wild type control.

Initial Cloning of Partial AtPK220L292F and AtPK220 Sequences

[0100] Based on the TAIR annotation, partial sequences of AtPK220L292F (AtPK220L292F(p)) and partial AtPK220 (AtPK220(p)) were amplified by RT-PCRs using the primers SEQ ID NO:106 and SEQ ID NO:107 which included BamHI and PstI restriction sites for cloning and template RNA isolated from hwe116 and the control plant (Columbia), respectively). The resulting partial AtPK220L292F nucleotide sequence is shown as SEQ ID NO:5 and the corresponding amino acid sequence as SEQ ID NO:6. The resulting partial AtPK220 nucleotide sequence is shown as SEQ ID NO:7 and the corresponding amino acid sequence as SEQ ID NO:8.

Kinase Activity Assay of a Partial AtPK220L292F Protein Expressed in E. coli

[0101] The PCR products were digested with BamHI and PstI, and inserted into the expression vector: pMAL-c2 (New England Biolabs, Beverly, Mass.) to form an in-frame fusion protein with the malE gene for expression of the maltose-binding protein: MBP-AtPK220L292F(p) and MBP-AtPK220(p). The fusion proteins were expressed in E. coli and purified using amylose-affinity chromatography as described by the manufacturer (New England Biolabs). Fractions containing the fusion proteins were pooled and concentrated (Centriprep-30 concentrator, Amicon). SDS-PAGE was used to analyze the expression level, size and purity of the fusion proteins.

[0102] Activity assays were carried out according to (Huang et al., 2000). The kinase autophosphorylation assay mixtures (30 .mu.l) contained kinase reaction buffer (50 mM Tris, pH 7.5, 10 mM MgCl.sub.2, 10 mM MnCl.sub.2), 1 .mu.Ci [.gamma.-.sup.32P] ATP and 10 ng of purified AtPK220L292F(p) or MBP-AtPK220(p). For the trans-phosphorylation assays, myelin basic protein (3 .mu.g) was added to each assay. The reactions were started by the addition of the enzymes. After incubation at room temperature for 30 min, the reactions were terminated by the addition of 30 .mu.g of Laemmli sample buffer (Laemmli, 1970). The samples were heated at 95.degree. C. for 5 min and then loaded on a 15% SDS-polyacrylamide gel. The gels were stained with Coomassie blue R-250, then de-stained and dried. The .sup.32P-labeled bands were detected using Kodak X-Omat AR film.

[0103] The wild type MBP-AtPK220(p) fusion protein was able to phosphorylate the artificial substrate in the in vitro activity assay, indicating that the assay system was effective and the MBP-AtPK220(p) fusion protein was capable of activity. In contrast, the hwe116 mutant form, MBP-AtPK220L292F(p), was unable to catalyse phosphorylation of the model substrate. The single point mutation is sufficient to abolish activity of the AtPK220(p) gene from hwe116.

Isolation of Full-Length cDNA Sequence of AtPK220

[0104] The annotation of AtPK220 (At2g25220) in the TAIR database identifies a 5' start codon, termination signal and 3' UTR sequence. Analysis of the 5' portion of the annotated sequence suggested an alternative 5' sequence and start codon location. To determine the AtPK220 genes' 5' region and the likely start codon SMART RACE (Rapid Amplification of cDNA Ends, CloneTech) was performed.

[0105] A specific primer, SEQ ID NO:108, was designed for the 5' RACE and yielded a 450 bp PCR product. Sequence data obtained of the 450 bp 5' RACE product indicated that the TAIR annotation of AtPK220 was missing the 5' 186 bp that included 39 bp of 5' UTR sequence and 147 bp of coding sequence. An intron of 324 bp, located 8 bp upstream of the TAIR identified ATG start codon of AtPK220 was also missing from the genomic annotation in TAIR.

[0106] Compiling the 5' RACE results and TAIR database annotation yields the full-length cDNA of AtPK220 (SEQ ID NO:9). The sequence was determined to be 1542 bp in length, which included 39 bp of 5' UTR, 204 bp of 3'UTR, and 1299 bp of coding region. The AtPK220 coding region is identified as SEQ ID NO:1 and encodes a protein of 432 amino acids and is identified as SEQ ID NO:2. Comparison of AtPK220 to its closest homolog, At4g32000, shows an additional sequence of 51 bp is present in AtPK220, that includes the sequence of nucleotides 368-418 of SEQ ID NO:9. This sequence provides a target sequence for down-regulation constructs designed to specifically down-regulate the AtPK220 gene but not non-target genes such as At4g32000.

[0107] Sequence analysis of AtPK220 indicates that this Ser/Thr PK belongs to a receptor-like protein kinase family, possessing a signal peptide (1-29), an extracellular domain (30-67), a single transmembrane domain (68-88), an ATP-binding domain (152-175 as determined by Prosite) a Ser/Thr protein kinase active-site domain (267-279 as determined by the InterPro method) and an activation loop (289-298, 303-316).

Rescue of the hwe116 Mutant by AtPK220

[0108] Constructs for the expression of wild-type AtPK220 were generated and transformed into the hwe116 mutant. The construct was constitutively expressed from a CaMV 35S promoter and referred to as 35S-AtPK220.

35S-AtPK220

[0109] The primer pair SEQ ID NO:109 and SEQ ID NO:110 was used to amplify a fragment comprising the full length open reading frame (ORF) of AtPK220. The primer pair SEQ ID NO:111 and SEQ ID NO:110 was used to amplify a fragment comprising a portion of AtPK220 ORF. The amplified fragments were digested with restriction enzymes SmaI and BamHI and cloned into a pEGAD vector digested with the same restriction enzymes. The fragment comprising the full length open reading frame of AtPK220 resulting from the PCR and subsequent restriction digestion is disclosed as SEQ ID NO:10. The fragment comprising a portion of the AtPK220 ORF resulting from the PCR and subsequent restriction digestion is disclosed as SEQ ID NO:11.

[0110] The 35S-AtPK220 construct was transformed into Arabidopsis hwe116. The transgenic lines were recovered and advanced to T3 homozygous lines. These lines are tested for their drought tolerance and water use efficiency characteristics. The 35S-AtPK220 construct restores the wild type phenotypes.

T-DNA Knockout Lines and Physiology Assessment

[0111] SALK T-DNA knockout lines of AtPK220 and two close homologous genes in which are identified as TAIR Accession numbers AT4G32000 (SEQ ID NO:16) and AT5G11020 (SEQ ID NO:18) were obtained from ABRC and advanced to homozygosity. They are listed as follows;

[0112] AtPK220: SALK_147838;

[0113] AtPK32000 (AT4G32000): SALK_060167, SALK_029937 and SALK_121979;

[0114] AtPK11020 (AT5G11020): SAIL_1260_H05.

[0115] Analysis of gene expression levels by either RT-PCR or Northern analysis demonstrated that the target genes in the knockout lines was either significantly reduced or completely abolished. These knockout lines were used for physiological assessment. Only the knockout line of AtPK220 (SALK_147838) showed significant drought tolerance and higher water use efficiency, indicating that AtPK220 is the target gene and responsible for the water use efficiency phenotype of hwe116. The closely related genes AT4G32000 and AT5G11020 are not functionally redundant and inhibition of these genes is insufficient to generate the hwe116 phenotype.

Inhibition of the Protein Activity for PK220 in Arabidopsis

[0116] Inhibition of gene activity can be achieved by a variety of technical means, for example, antisense expression, RNAi or hairpin constructs, in vivo mutagenesis, dominant negative approaches or generation of a mutant population and selection of appropriate lines by screening means. Provided are examples of said means to produce plants having inhibited PK220 gene expression and or activity.

Down-Regulation of PK220 by RNAi

[0117] Constructs were designed for RNAi inhibition of PK220 using hairpin (HP) constructs. The constructs comprised a 288 bp or a 154 bp of AtPK220 cDNA sequence to produce constructs referred to as (270)PK220 and (150)PK220. The 288 bp (270)PK220 fragment comprises 10 bp of intron sequence that was included in the PCR primer during construction of these PCR products. Vector constructs using these fragments can be made to drive expression under the control of a promoter of choice that will be apparent to one of skill in the art. In these examples a constitutive promoter (35S CaMV), or the native AtPK220 promoter (P.sub.PK) was used. Two fragments, or portions, of the AtPK220 gene were selected, first a 288 bp fragment At(270)PK220 (SEQ ID NO:13) and second a 154 bp fragment At(150)PK220 (SEQ ID NO:12) were selected from a divergent region of AtPK220 as compared to its closest homologue At4g32000.

35S-HP-At(270)PK220 and 35S-HP-At(150)PK220

[0118] The hairpin constructs (HP) 35S-HP-At(270)PK220 and 35S-HP-At(150)PK220 constructs were generated as follows. The sense fragments of (270)PK220 and (150)PK220 were amplified by RT-PCR using primer pairs of SEQ ID NO:134/SEQ ID NO:115 and SEQ ID NO:114/SEQ ID NO:115, respectively. The PCR products were digested with SacI, and inserted into a binary vector pBI121tGUS at the SacI site, respectively. The resulting vectors were then used to subclone the antisense fragments of (270)PK220 and (150)PK220 that were derived from RT-PCR products amplified using primer pairs of SEQ ID NO:112/SEQ ID NO:117, and SEQ ID NO:116/SEQ ID NO:117, respectively. Both the vector and PCR products were digested with BamHI and XbaI for subcloning.

P.sub.PK-HP-At(270)PK220 and P.sub.PK-HP-At(150)PK220

[0119] The P.sub.PK-HP-At(270)PK220 and P.sub.PK-HP-At(150)PK220 constructs were made from 35S-HP-At(270)PK220 or 35S-HP-At(150)PK220 respectively by replacing the 35S promoter sequence with AtPK220 promoter sequence (SEQ ID NO:14). The 35S promoter sequence was removed from 35S-HP-At(270)PK220 and 35S-HP-At(150)PK220 by Hind III and Xba I double digestion. The linearized plasmid was then treated with Klenow fragment of DNA polymerase I to generate blunt ends and self-ligated to form a new plasmid, in which XbaI site was restored while Hind III was gone. By using this restored XbaI site, a Nhe I DNA fragment of AtPK220 promoter was cloned upstream of HP-At(270) and HP-At(150) sequence to produce the final plasmids of P.sub.PK-HP-At(270)PK220 and P.sub.PK-HP-At(150)PK220. AtPK220 promoter sequence (SEQ ID NO:14) was amplified by PCR from Arabidopsis (Columbia) genome using primer pairs of SEQ ID NO:135/SEQ ID NO:136.

P.sub.4790-HP-At(270)PK220

[0120] To specifically down-regulate endogenous AtPK220, a strong root promoter P.sub.4790 was identified and found to be highly expressed in the roots of Arabidopsis, particularly in the endodermis, pericycle, and stele. The P.sub.4790 promoter is associated with a coding sequence identified as At2g44790 and the expression characteristics of P.sub.4790 are similar to that of wild type AtPK220 expression. The P.sub.4790 was used to replace the constitutive 35S promoter in 35S-HP-At(270)PK220. The promoter of At2g44790 was amplified using Arabidopsis (Col) genomic DNA as template and primers SEQ ID NO:151 and SEQ ID NO:152. The amplified promoter fragment has the length of 1475 base-pairs right upstream the ATG start codon of At2g44790 according to TAIR annotation. The 1475 bp-P.sub.4790 fragment is identified a SEQ ID NO:150. Hind III and Xba I restriction sites were introduced to the 5' and 3' end of the promoter fragment by primer design. The promoter sequence was then used to replace the 35S promoter in 35S-HP-At(270)PK plasmids by HindIII/XbaI double digestion, which resulted in the final constructs of pBI-P.sub.4790-HP-At(270)PK.

Down-Regulation of BnPK220 in Brassica Using RNAi

35S-HP-Bn(340)PK

[0121] To down-regulate the AtPK220 homolog in Brassica species, a hairpin construct was made using a 338 bp fragment of BnPK220 (SEQ ID NO;153) as the sense and anti-sense portions, and pBI300tGUS as the vector. Two pairs of primers SEQ ID NO:154 and SEQ ID NO:155; and SEQ ID NO:156 and SEQ ID NO:157 with unique restriction sites were designed according to BnPK220 sequence. A PCR fragment of 338 bp in length was amplified using Brassica napus cDNA as the template and the two pairs of primers, respectively. The SacI fragment was then inserted into pBI300tGUS at the SacI site downstream of the tGUS spacer in an antisense orientation. The resulting plasmid was subsequently used for cloning of a XbaI-BamI fragment in a sense orientation at the XbaI and BamHI sites. The vector pBI121tGUS was modified within the NPT II selectable marker gene and named pBI300. The NPT II gene in the vector pBI121 contains a point mutation (G to T at position 3383, amino acid change E182D). To restore the gene with its WT version, the NheI-BstBI fragment (positions 2715-3648) was replaced with the corresponding NheI-BstBI fragment from plasmid pRD400 (PNAS, 87:3435-3439, 1990; Gene, 122:383-384, 1992).

P.sub.4790-HP-Bn(340)PK

[0122] The P.sub.4790 promoter of At2g44790 was used to control expression of a hairpin construct to down-regulate endogenous BnPK220 in Brassica. The plasmid of 35S-HP-Bn(340)PK was digested with HindIII and XbaI to replace the 35S promoter with the P.sub.4790 promoter.

Down-Regulation of PK220 by Antisense

[0123] The construct 35S-antisenseAtPK220 was made to down-regulate expression of AtPK220 via antisense. The antisense fragment was generated using PCR and the primer pair SEQ ID NO:106/SEQ ID NO:113. The synthesised product was digested with BamHI and XbaI to yield a 1177 bp sequence comprising 1160 bp of AtPK220 (SEQ ID NO:11). Included at the 5' end were 10 bp of intron sequence and at the 3' end, 7 bp of 3' UTR sequence, which were retained from the PCR primers. The 1177 bp fragment was cloned in an antisense orientation to the 35S promoter in pBI121w/oGUS at the BamHI and XbaI.

Down-Regulation of PK220 by AmiRNA

[0124] An artificial microRNA (amiRNA) construct was also made to down-regulate the expression of AtPK220 in Arabidopsis. An Arabidopsis genomic DNA fragment containing microRNA319a gene (SEQ ID NO:148), was amplified by PCR using Arabidopsis (Col) genomic DNA as template and primers listed as SEQ ID NO:141 and SEQ ID NO:142. The backbone of miR319a was then used to construct amiRPK220 (SEQ ID NO:149), in which a 21 bp fragment of miRNA319a gene in both antisense and sense orientations was replaced by a 21 bp DNA fragment of AtPK220 using recombinant PCR. Three pairs of primers: SEQ ID NO:141/SEQ ID NO:144; SEQ ID NO:143/SEQ ID NO:146 and SEQ ID NO:145/SEQ ID NO:142 were designed for the construction. The final PCR product was digested with BamHI and XbaI, and subsequently cloned into pBI121w/o GUS for transformation into Arabidopsis or other plant species of choice.

Inhibition of PK220 Via Dominant-Negative Strategy

35S-AtPK220L292F

[0125] For expression of a non-functional AtPK220 sequence the AtPK220L292F from hwe116 was PCR amplified by RT-PCR using forward and reverse primers SEQ ID NO:118 and SEQ ID NO:110. The PCR product was digested with the restriction enzymes BamHI and XbaI (SEQ ID NO:121) and ligated into the binary vector pBI121w/oGUS. The sequence of SEQ ID NO:121 comprises the AtPK220L292F open reading frame (SEQ ID NO:3) and an additional 3 bp at the 5' end and 7 bp at the 3' end that are derived from UTR sequences (SEQ ID NO:121). The final construct, 35S-AtPK220L292F, was used to generate Arabidopsis and Brassica transgenic plants that were advanced to homozygosity for physiology assessment. Additionally, the vector is used to transform a plant species of choice and can be a dicot or a monocot.

P.sub.4790-AtPK220L292F

[0126] The HindIII-XbaI fragment of the root promoter P.sub.4790 was used to replace 35S promoter in pBI300, and then AtPK220L292F sequence was put downstream P.sub.4790 by XbaI and BamHI digestion to generate the P.sub.4790-driven dominant-negative construct. The resulting plasmid was then used for Brassica transformation. Additionally, the vector is used to transform a plant species of choice and can be a dicot or a monocot.

Down-Regulation of AtPK220 Homologs in a Monocot Species Using RNAi P.sub.BdUBQ-HP-Bd(272)PK

[0127] An expression cassette was constructed and inserted into two different vector backbones, the first being into the PacI-AscI sites of pUCAP and the second being into the PacI-AscI sites of pBF012. pBF012 is identical to pBINPLUS/ARS except that the potato-Ubi3 driven NPTII cassette has been excised via FseI digestion followed by self-ligation.

[0128] Brachypodium distachyon PK220 (BdPK220) was amplified using primer combinations SEQ ID NO:158 (bWET XbaI F) plus SEQ ID NO:159 (bWET BamHI R) having XbaI or BamHI sites respectively in the primers and SEQ ID NO:158 (bWET XbaI F) plus SEQ ID NO:160 (bWET ClaI R) having XbaI or ClaI sites respectively in the primers. PCR products were digested with the indicated restriction enzymes giving a 272 bp fragment (SEQ ID NO:161).

[0129] The hairpin spacer sequence, BdWx intron 1 (SEQ ID NO:164), was amplified with SEQ ID NO:162 (bWx BamHI F) plus SEQ ID NO:163 (bWx ClaI R) primers having BamHI or ClaI sites respectively in the primers and digested with the indicated restriction enzymes. The B. distachyon Wx gene is a homologue of the rice GBSS waxy gene, although the introns show little conservation.

[0130] The three fragments were ligated together into the XbaI site of the pUCAP MCS resulting in BdWx intron 1 sequence being flanked by Bd(272)PK220 target sequences in opposite orientations. The B. distachyon ubiquitin (BdUBQ) promoter contains an internal BamHI site, so the RNAi cassette was amplified with primers SEQ ID NO:200 (bWET BamHI end1) and SEQ ID NO:165 (bWET BamHI end2) which create BamHI cohesive ends without the need for BamHI digestion. The BamHI RNAi fragment was then ligated into the BamHI site of pUCAP already containing BdUBQ promoter and BdUBQT terminator resulting in the intermediate clone pBF067. The pBF067 complete insert was amplified with SEQ ID NO:166 (BdUBQ PvuI F) and SEQ ID NO:167 (BdUBQT PacI R), digested with PvuI and PacI and subsequently ligated into the PacI site of pUCAP or pBF012 vectors already containing a BdGOS2 driven mutant NPTII selectable marker in the AscI-PacI sites, resulting in pBF108 and pBF109, respectively. This mutant NPTII gene is commonly found in cloning vectors. There is only a single base pair difference from the wild type.

[0131] This cassette is in the PacI-AscI sites of pUCAP for the shuttle/bombardment vector pBF108 and in the PacI-AscI sites of pBF012 for the binary vector pBF109.

P.sub.BdUBQ-HP-Pv(251)PK

[0132] An expression cassette was constructed and inserted into two different vector backbones, the first being into the PacI-AscI sites of pUCAP and the second being into the PacI-AscI sites of pBF012. A fragment of Panicum virgatum PK220 being 251 bp in length (Pv(251)PK220) and identified as SEQ ID NO:168 was amplified using primer combinations SEQ ID NO:169 (PvWET XbaI F) plus SEQ ID NO:170 (PvWET BamHI R) and SEQ ID NO:169 (PvWET XbaI F) plus SEQ ID NO:171 (PvWET ClaI R). PCR products were digested with the indicated restriction enzymes. No sequence information exists regarding the PvWx intron 1 so the BdWx intron 1 was used as the spacer sequence in this construct. This sequence was amplified with SEQ ID NO:162 (bWx BamHI F) plus SEQ ID NO:163 (bWx ClaI R) primers and digested with the indicated restriction enzymes.

[0133] The three fragments were then ligated together into the XbaI site of the pUCAP MCS resulting in BdWx intron 1 sequence being flanked by Pv(251)PK220 target sequences in opposite orientations. No PvUBQ promoter sequence was available so the BdUBQ promoter and terminator are used in this construct. The BdUBQ promoter contains an internal BamHI site, so the RNAi cassette was amplified with primers SEQ ID NO:172 (PvWET BamHI end1) and SEQ ID NO:173 (PvWET BamHI end2) which create BamHI cohesive ends without the need for BamHI digestion. The BamHI RNAi fragment was then ligated into the BamHI site of pUCAP already containing BdUBQ promoter and BdUBQT terminator resulting in the intermediate clone pBF152. The pBF152 complete insert was amplified with SEQ ID NO:166 (BdUBQ PvuI F) and SEQ ID NO:167 (BdUBQT PacI R), digested with PvuI and PacI and subsequently ligated into the PacI site of pUCAP or pBF012 vectors already containing BdGOS2 driven wildtype NPTII in the AscI-PacI sites, resulting in pBF169 and pBF170, respectively.

P.sub.SbUBQHP-Sb(261)PK

[0134] An expression cassette was constructed and inserted into two different vector backbones, the first being into the PacI-AscI sites of pUCAP and the second being into the PacI-AscI sites of pBF012. A fragment of Sorghum bicolor PK220 (SbPK220) being 261 bp in length (Sb(261)PK220) and identified as SEQ ID NO:174 was amplified using primer combinations SEQ ID NO:175 (SbWET XbaI F) plus SEQ ID NO:176 (SbWET BamHI R) and SEQ ID NO:175 (SbWET XbaI F) plus SEQ ID NO:177 (SbWET ClaI R). PCR products were digested with the indicated restriction enzymes to give a Sb(261)PK220 fragment. The hairpin spacer sequence, SbWx intron 1 (SEQ ID NO:178), was amplified with primers SEQ ID NO:179 (SbWx BamHI) plus SEQ ID NO:180 (SbWx ClaI R) and digested with the indicated restriction enzymes. The three fragments were then ligated together into the XbaI site of the pUCAP MCS resulting in SbWx intron 1 sequence being flanked by SbWET target sequences in opposite orientations. BamHI cohesive ends were added to the RNAi cassette via amplification with primers SEQ ID NO:181 (SbWET BamHI end1) and SEQ ID NO:182 (SbWET BamHI end2). The BamHI RNAi fragment was then ligated into the BamHI site of pUCAP already containing SbUBQ promoter and SbUBQT terminator resulting in the intermediate clone pBF151. The pBF151 complete insert was amplified with SEQ ID NO:192 (SbUBQ PvuI F) and SEQ ID NO:167 (BdUBQT PacI R), digested with PvuI and PacI and subsequently ligated into the PacI site of pUCAP or pBF012 vectors already containing BdGOS2 driven wildtype NPTII in the AscI-PacI sites, resulting in pBF158 and pBF171, respectively.

[0135] A SbGOS2 promoter was identified from the Sorghum genome sequence was amplified and using the primer pair SEQ ID NO:184 (SbGOS2 HindIII F) and SEQ ID NO:185 (SbGOS2 HindIII R) a 1000 bp fragment of the GOS2 promoter, identified as SEQ ID NO:183, was PCR amplified and cloned using the HindIII restriction sites.

[0136] A SbUBQ promoter was identified from the Sorghum genome sequence was amplified and using the primer pair SEQ ID NO:187 (SbUBQ PstI F) and SEQ ID NO:188 (SbUBQ PstI R) a 1000 bp fragment of the UBQ promoter, identified as SEQ ID NO:186, was PCR amplified and cloned using the PstI restriction sites.

[0137] A SbUBQ terminator was identified from the Sorghum genome sequence was amplified and using the primer pair SEQ ID NO:190 (SbUBQT KpnI F) and SEQ ID NO:191 (SbUBQT KpnI R) a 239 bp fragment of the UBQ terminator, identified as SEQ ID NO:189, was PCR amplified and cloned using the KpnI restriction sites.

Miscanthus giganteus (MgPK220) RNAi

[0138] Expression constructs designed to down regulate via a hairpin strategy can be devised following the same strategy as described above. Resulting in a construct that may comprise the following elements, a BdGOS2-wtNPTII-BdUBQT selectable marker cassette and a BdUBQ-(MgPK220 hairpin-RNAi cassette)-BdUBQT in a vector of choice such as pUCAP and pBF012

AtPK220 Promoter Isolation and Cloning

[0139] The AtPK220 promoter was isolated using a PCR approach using Arabidopsis (Columbia ecotype) genomic DNA as template. The 5' primer, SEQ ID NO:119, was designed near the adjacent gene and the 3' primer, SEQ ID NO:120, located 25 bp upstream of the ATG start codon of the AtPK220 gene. The amplified product was digested with BamHI and SmaI and cloned into pBI101. The digested fragment, SEQ ID NO:14, was 1510 bp in length. The resulting construct was named P.sub.AtPK220-GUS.

AtPK220 Promoter Activity Analysis Using GUS Assay

[0140] P.sub.AtPK220-GUS was transformed into Arabidopsis plants using flower dipping, and the transgenic plants were advanced to T3 homozygorsity. Various tissues including young seedlings and leaves, stems, flowers, siliques, and roots from T3 flowering plants were collected, stained in X-Gluc solution at 37 C overnight, de-stained with ethanol solution, and examined under a microscope. The results showed that the promoter of AtPK220 was expressed mainly in endodermis and pericycle cells of root tissue and was also found in leaf trichomes and seed coat of developing seeds. Of significance was the observation that expression of P.sub.AtPK220-GUS was suppressed by water stress.

Sub-Cellular Localisation of AtPK220 Proteins in Arabidopsis

[0141] Expression of a full length wild type AtPK220-GFP fusion protein in transgenic Arabidopsis was used to locate the sub-cellular localization of the native protein. The primer pair SEQ ID NO:109 and SEQ ID NO:110 produced a fragment that was digested with SmaI and BamHI to yield a fragment comprising the full length open reading frame of AtPK220 and is disclosed as SEQ ID NO:10 and cloned downstream, in frame with the green fluorescence protein (GFP) in a pEGAD plasmid at the SmaI and BamHI sites. Additionally, the AtPK220 coding sequence was amplified using primer pair SEQ ID NO:198 and SEQ ID NO:199 and inserted upstream and in frame with GFP by AgeI digestion of pEGAD plasmid and the amplified AtPK220 fragment.

[0142] The 35S-GFP-AtPK220 and 35S-AtPK220-GFP constructs were transformed into Arabidopsis plants and homozygous transgenic plants (root tissues) were used for visual screening of GFP signal under confocal microscope. Green fluorescence was detected along plasma membrane, suggesting that AtPK220 protein was associated with plasma membrane in roots and that AtPK220 possibly functions as receptor kinase to sense or transduce environmental signals.

Isolation of BnPK220 from Brassica napus by 5' and 3' RACE

[0143] To isolate the homologous gene of AtPK220 from canola, a blast search (BLASTn) of NCBI Nucleotide Collection (nr/nt, est) and TIGR (DFCI) Brassica napus EST Database was done using AtPK220 sequence. Based on the sequences with highest similarity, a pair of primers, SEQ ID NO:122 and SEQ ID NO:123 were designed and used to PCR amplify a partial fragment of BnPK220. Both mRNA and genomic DNA isolated from Brassica leaves were used as template for these amplifications. A DNA fragment of about 500 bp was obtained by PCR from canola genomic DNA template. Sequence analysis of this PCR product showed that it shares a high identity with AtPK220 in nucleotide sequence as well in the intron organisation.

[0144] Based on the partial sequence of BnPK220, 5' and 3' RACE was performed to isolate the full length BnPK220 cDNA. For 3' RACE a forward primer, SEQ ID NO:124 and a nested primer, SEQ ID NO:125, were used. For 5' RACE a reverse primer, SEQ ID NO:126, and its nest primer, SEQ ID NO:127, were designed. RACE-ready cDNA for either 5' RACE or 3' RACE was made from RNA isolated from young Brassica leaves.

[0145] The 5' RACE yielded an amplified DNA of about 650 bp in length; and 3' RACE yielded a DNA of about 1 kb in size. Sequencing of these two RACE fragments showed high sequence similarity with AtPK220. A full-length mRNA of BnPK220 sequence was assembled by combining 5'RACE, partial BnPK220 fragment and 3' RACE results.

[0146] A full length BnPK220 cDNA was amplified by RT-PCR using the PCR primers SEQ ID NO:128 and SEQ ID NO:129. This cDNA comprises an ORF of 1302 nucleotides (SEQ ID NO:25) and encodes a protein of 433 amino acids (SEQ ID NO:26). Another full length BnPK220 cDNA was also amplified by the RT-PCR using cDNA made from B. napus. This cDNA (SEQ ID NO: 193) is 98.6% identical to SEQ ID NO:25, and encodes a protein (SEQ ID NO:194) of 99.3% identical to SEQ ID NO:26.

Isolation of Full-Length GmPK220 from Soybean by 5' RACE

[0147] A Blastn search of NCBI EST database, a homolog of AtPK220 was found as a soybean (Glycine max) EST, CX709060.1. From this homolog, a unigene cluster of 13 ESTs was retrieved from a soybean EST database. A contig was then assembled from these 13 ESTs, which covers a majority of the gene sequence.

[0148] The full-length sequence of GmPK220 (SEQ ID NO:41) was determined by combining the assembled contig, 5' RACE and 3' RACE results. The 5' RACE was performed using the primers of SEQ ID NO:130 for primary RACE PCR and SEQ ID NO:131 for nested RACE PCR. The 3' RACE was performed using the primers of SEQ ID NO:137 for primary RACE PCR and SEQ ID NO:138 for nested RACE PCR. GmPK220 encodes a protein as shown in SEQ ID NO:42.

Isolation of OsPK220 (Rice) Sequence by Database Mining

[0149] The rice genome (Oryza sativa, japonica cultivar) has been completely sequenced and is publically available. The homolog of AtPK220 in rice was determined by BLAST search of a rice EST database and by BLASTP search of a genomic sequence database. The target having the highest score was identified as Accession number Os05g0319700.

[0150] Os05g0319700 is abbreviated as OsPK220, and disclosed as SEQ ID NO:59, which encodes a protein disclosed as SEQ ID NO:60.

Isolation of ZmPK220 (Corn) Sequence

[0151] Two candidate homologs were found by BLAST search of the TIGR EST database, one a unigene Accession number TC333547 and the second Accession number C0439063.

[0152] Accession number TC333547 is 2125 nucleotides in length and contains an open reading frame of 1377 nucleotides (SEQ ID NO:77) encoding a protein of 458 amino acids (SEQ ID NO:78). This translated protein is full-length and is larger than AtPK220 protein. The C-terminal kinase domain is highly conserved between the Arabidopsis and corn protein sequence, however, the N-terminal sequence is more variable.

[0153] C0439063 is a short EST sequence and is missing 5' terminal sequence. The missing sequence was obtained by RACE methods. Two 5' RACE primers were designed based on the alignment between AtPK220 and C0439063. The primary 5' RACE primer is SEQ ID NO:132 and the nested 5' RACE primer is SEQ ID NO:133. The 3' RACE was also performed using the primers of SEQ ID NO:139 for primary RACE PCR and SEQ ID NO:140 for nested RACE PCR. The ZmPK220 (SEQ ID NO:79) sequence was assembled based on 5' RACE, 3' RACE results and C0439063 EST sequences. The corresponding protein sequence was listed as SEQ ID NO:80.

[0154] Sequence analysis shows that C0439063 has higher sequence similarity with rice OsPK220 than TC333547.

Isolation of BdPK220 Sequence from Brachipodium Distachyon (Bd)

[0155] Brachipodium is one of the model monocot plants for functional genomic research. A contig was assembled from public ESTs or GSSs, and it covers a 3' portion of BdPK220 according to homologue alignment. RACE using Bd81RAR1 primer (SEQ ID NO: 195) and Bd81RAR2 primer (SEQ ID NO: 196) designed from the contig and using Brachipodium leaf cDNA produced a unique fragment of about 650 bp. The assembling of the RACE sequence and the contig gave the full length BdPK220 sequence (SEQ ID NO:24), which encodes a protein of 461 amino acids (SEQ ID NO: 197).

Determination of GsPK220 (Cotton) Sequence by Database Mining

[0156] A BLAST search of a cotton (Gossypium) TIGR-EST database identified a sequence cluster identified as Accession number TC79117, that has high similarity with AtPK220. This cluster has two overlapping ESTs, TC79117 which is referred herein as GsPK220) and consists of an open reading frame of 1086 nucleotides (SEQ ID NO:81). The largest open reading frame encodes a protein of 361 amino acids (SEQ ID NO:82).

Drought Tolerant Phenotype of hwe116 Mutant Found Under Water Limited Conditions and High Water Use Efficiency Under Both Drought and Optimal Conditions

[0157] Two groups of plants were grown (5 plants per 3'' pot filled with the same amount of soil-less mix) under optimal conditions in a growth chamber (22 C, 18 hr light, 150 uE, 70% relative humidity) until first day of flower (n=6 per entry per treatment). At first flower all plants were supplied with the same amount of water (optimal levels) but one group of plants was used for the optimal treatment and the other for drought treatments. In the optimal treatment the pots were weighed daily to determine daily water loss and then watered back up to optimal levels. In the drought treatment, pots were weighed daily to determine water loss and allowed to dry out. Plants were harvested on days 0, 2 and 4 of drought and optimal treatments for shoot biomass determinations. Lower water loss relative to shoot dry weight (DW) as compared to control, under drought conditions indicates a drought tolerant phenotype. The ratio of shoot dry weight accumulated to water lost during the treatment period provides a measure of water use efficiency (WUE). The hwe116 plants were delayed in flowering by 1 to 2 days. Water loss relative to shoot biomass was significantly lower (by 22%) in hwe116 than parent control under drought conditions. This result indicates that the mutant is drought tolerant. It has also been found that under optimal conditions the water loss relative to shoot DW was also significantly lower in the mutant (by 41%) as compared to the parent control. This result is consistent with higher water use efficiency phenotype. Calculations of water use efficiency showed that under both drought (Table 1) and optimal (Table 2) conditions hwe116 mutant uses water more efficiently because it accumulated more shoot biomass with less water (drought) or the same amount of biomass with less water (optimal).

TABLE-US-00001 TABLE 1 Water Use Efficiency (WUE) under drought conditions shoot DW WUE accumulated- water lost - (g shootDW acc/ Entry day 0 to 4 (g) day 0 to 4 (g) kg water lost) hwe116 0.146 56.5 2.58 (+13%) Parent 0.134 58.6 2.28

TABLE-US-00002 TABLE 2 Water Use Efficiency (WUE) under optimal conditions shoot DW accumulated- water lost - WUE (g shootDW acc/ entry day 0 to 4 (g) day 0 to 4 (g) kg water lost) hwe116 0.276 92.3 2.99 (+22%) Parent 0.271 110.6 2.45

[0158] The final result of enhanced water use efficiency in the mutant is greater shoot DW biomass as shown in Table 3 (harvested on day 4 from 1.sup.st flower).

TABLE-US-00003 TABLE 3 Final shoot DW biomass Drought - Optimal - shoot DW (g) shoot DW (g) entry Mean S.E. Mean S.E. hwe116 0.354 0.014 0.449 0.017 parent 0.300 0.011 0.414 0.011 hwe116 as % of parent 118% 108%

The hwe116 Mutant Maintains Higher Soil Water Content During Drought Treatment, Reaches Water-Stress Conditions Later and Shows Yield Protection Following Drought Stress During Flowering Relative to Control Plants.

[0159] An experiment was set up with 5 plants per 4'' pot filled with the same amount of soilless mix. Two groups of plants (optimal and drought) were grown under optimal conditions in a growth chamber (22 C, 18 hr light, 150 uE, 70% relative humidity) until first day of flower (n=9 per entry and per group). At first flower all plants were supplied with the same amount of water and further water was withdrawn for the drought treated group of plants. The optimal group was watered daily as before. Pots in the drought treated group were weighed daily for 6 days of treatment to determine soil water content. After 6 days of drought treatment plants were re-watered and allowed to complete their lifecycle as the optimal group under optimal conditions. At maturity the seeds were harvested from each pot and the seed yield was determined for both optimal and drought treated plants. The results of changes in soil water content during the drought treatments were determined. Soil water content was measured as percentage of initial amount of water in the pot. The results indicate that the mutant was able to retain water in pots longer and therefore it reached the stress level (around 25% soil water content) 1 day later and wilted 1 day later than control. This treatment caused a yield reduction of 17% from optimal levels in the mutant, whereas in control the yield reduction was 41%. Therefore the mutant demonstrated a yield protection of 24% relative to control, following a drought treatment.

The hwe116 Mutant Seedlings Showed Less Sensitivity to Cold Stress.

[0160] Two groups of plants with 8 replicates per entry were grown with 3 plants per 3'' pot under optimal conditions of 22.degree. C. and short days to prolong vegetative growth and delay flowering (10 hr light 150 uE, and 14 hr dark), 70% relative humidity in a growth chamber. At 10 days of age (3 days post-transplanting of seedlings into soil from agar plates) the cold treatment group was placed in a chamber at 8.degree. C. for 11 more days of growth while the optimal group was maintained at 22.degree. C. Plants were harvested for shoot dry weight (DW) determinations at 21 days of age. The results are shown in Table 4. The hwe116 mutant had smaller seedlings under optimal conditions than those of controls but after cold exposure the shoot DW was equivalent to that of the parent and as percentage of the optimal DW it was higher than that of both controls by 9 and 15% indicating that the growth of the mutant was not as inhibited by cold as that of controls.

TABLE-US-00004 TABLE 4 shoot dry weight under optimal and cold conditions. optimal (22.degree. C.) Cold (8.degree. C.) shoot DW (mg) shoot DW (mg) shoot DW Entry Mean S.E. Mean S.E. % of optimal hwe116 6.65 0.30 2.85 0.13 43% parent 9.16 0.21 2.58 0.11 28% WT 9.30 0.20 3.18 0.21 34%

The hwe116 Mutant has Thicker Leaves and Higher Chlorophyll Content Per Leaf Area. The Mutant Showed Delayed Leaf Senescence and Resistance to Oxidative Stress.

[0161] Plants were grown 1 per 3'' pot under optimal growth conditions in a growth chamber (16 hr light, 300 uE, 22.degree. C., 70% relative humidity). Early into flowering three leaf disks (86.6 um2 each) were taken from three youngest fully developed leaves and placed in petri dishes containing filter paper with 5 uM N,N'-Dimethyl-4,4'-bipyridinium dichloride (paraquat) solution as an oxidizing agent. Plates with leaf disks were placed under continuous light of 150 uE for 25 hours. This resulted in chlorophyll bleaching. The differences between the mutant and controls in the extent of bleaching were quantified by measuring chlorophyll content of the leaf disks. A leaf disk was also removed from leaves that have not been exposed to paraquat treatment and optimal chlorophyll content was determined. These disks were also weighed. The results showed that the mutant had higher total chlorophyll content per leaf surface area (Table 5), however the leaves of this mutant are thicker (leaf disks were 15 to 24% heavier in the mutant compared to those of controls). Chlorophyll content per gram of fresh leaf tissue was, therefore, not different. There were no differences between chlorophyll a to b ratios between the mutant and controls. The hwe116 mutant showed resistance to the oxidative stress as indicated by 5 to 7% higher chlorophyll content following paraquat treatment (Table 5). Leaf senescence was also delayed in the hwe116 mutant (data not shown).

TABLE-US-00005 TABLE 5 Effect of oxidative stress on chlorophyll content of leaves. Optimal 5 uM paraquat in 24 hr light Chl (a + b) - (mg/m2) Chl (a + b) - (mg/m2) % of Entry Mean Std Err Mean Std Err opt hwe116 303.7 6.7 67.9 4.4 20% Parent 259.6 4.3 39.5 5.9 15% WT 250.2 5.7 32.1 2.9 13%

The Growth of Mutant hwe116 Seedlings Showed Less Inhibition on Low Nitrogen Containing Media.

[0162] Twelve seedlings were grown on an agar plate (6 plates per entry) containing 1/2 MS growth media with optimal (20 mM) or low (0.3 mM) nitrogen content. Plates were placed in a growth room with an 18 hr light period (100 uE) for 6 days in a vertical position, then plates were placed horizontally and seedlings were grown for another 4 days before the shoots were harvested. The average seedling shoot DW after 10 days of growth was calculated per plate. The results are shown in Table 6. The shoot DW of hwe116 mutant grown under optimal conditions was significantly reduced but when grown on low nitrogen there were no differences. The shoot DW on low nitrogen in the mutant was 3 to 7% greater than in controls when compared to the optimal nitrogen levels. This indicates that the mutant may have better nitrogen use efficiency.

TABLE-US-00006 TABLE 6 Effect of nitrogen on seedling shoot DW Average seedling shoot DW (mg) Optimal nitrogen Low nitrogen Entry Mean S.E. Mean S.E. % Opt hwe116 1.03 0.03 0.23 0.01 22 Parent 1.34 0.04 0.20 0.01 15 WT 1.22 0.03 0.23 0.02 19

Knockout Mutant of PK220 Showed Drought Tolerant Trends and Higher Water Use Efficiency Under Drought Treatment.

[0163] Plant lines obtained from the SALK institute that were T-DNA knockouts in the AtPK220 gene (SALK_147838) were grown (5 per 3''pot) under optimal conditions in a growth chamber (18 hr light, 150 uE, 22.degree. C., 60% relative humidity) until first open flower (n=8 per entry and per harvest). The drought treatment was started by watering all plants with the same amount of water and cessation of further watering. Pots were weighed daily and plants were harvested for shoot DW determinations on days 0, 2 and 4 of the drought treatment. The result showed that water lost from pots in 2 days relative to shoot DW on day 2 was significantly lower (by 13%) for the knockout mutant and its shoot DW was also significantly greater (by 24%) on day 2 as compared to control wild-type. This result is consistent with drought tolerant phenotype.

[0164] The results showed that the water use efficiency of the knockout mutant was greater than that of the control-WT as the knockout mutant was able to accumulate more shoot biomass in the 2 days of treatment while using the same amount of water as control (Table 7).

TABLE-US-00007 TABLE 7 Water use efficiency under drought treatment WUE entry g water lost g shoot DW gain (g shoot/kg water) PK220- 43.1 0.059 1.37 knockout WT 42.9 0.035 0.82

Transgenic Lines of 35S-HP-At(270)PK220 Construct in Arabidopsis Showed Drought Tolerance.

[0165] Plants were grown (5 per 3'' pot and 8 pots per entry per harvest) under optimal conditions in a growth chamber (18 hr light, 150 uE, 22.degree. C., 60% relative humidity) until first day of flower. The drought treatment was started by watering all pots with the same amount of water and cessation of further watering. Pots were weighed daily for water loss determinations and plants were harvested for shoot biomass on day 4 of drought treatment. The results (Table 8) showed that 11 out of 13 transgenic lines demonstrated a drought tolerant phenotype (having a lower water loss over 2 days relative to shoot biomass on day 4). Four of the lines showed a slight delay in flowering (1 day), as did the hwe116 mutant. The final shoot biomass on day 4 was greater for most of the transgenic lines as compared to control WT. These results are indicative of a drought tolerant phenotype in the transgenic lines down-regulated in PK220 expression. As examples, the reduction in expression level of AtPK220 for the top 3 performing lines: 65-4, 38-5, and 59-3, are 75%, 47% and 58%.

TABLE-US-00008 TABLE 8 Drought tolerance and shoot DW (day 4) for 35S-HP-At(270)PK220 transgenic lines relative to wild type (WT) and the hwe116 mutant relative to parent control. drought tolerance shoot DW entry % of control % of control 65-4 119% 132% 38-5 116% 124% 59-3 112% 119% 33-7 111% 114% 54-11 108% 115% 56-3 107% 115% 43-11 107% 113% 23-8 106% 111% 12-2 106% 110% 63-4 104% 110% 32-1 104% 109% 30-3 101% 104% 74-2 101% 107% WT 100% 100% hwe116 186% 106% parent 100% 100%

Drought Tolerance of 35S-HP-At(270)PK220 Transgenic Lines in Arabidopsis and Enhanced Water Use Efficiency were Confirmed.

[0166] The transgenic lines of 35S-HP-At(270)PK220 were grown with 5 per 3'' pot under optimal conditions in a growth chamber (18 hr light, 150 uE, 22.degree. C., 60% relative humidity) until first flower (n=8). Drought treatment was started at first flower by watering all the pots with the same amount of water and cessation of further watering. The pots were weighed daily for the 4 days of drought treatment and plants were harvested on days 0, 2 and 4 of treatment. The results confirmed that water lost in 2 days relative to shoot biomass on day 2 was lower in five transgenic lines relative to controls, confirming their drought tolerant phenotype (Table 9). The shoot DW on day 2 was greater in 5 of the transgenic lines.

TABLE-US-00009 TABLE 9 Drought tolerance and shoot DW for 35S- HP-At(270)PK220 transgenic lines drought tolerance shoot DW entry % of WT % of WT 59-3 110% 105% 65-4 110% 98% 38-5 107% 109% 33-7 103% 106% 56-3 102% 95% 54-11 101% 103% null (65-1) 99% 99% WT 100% 100%

[0167] The water use efficiency was greater than that of controls during the 4 days of drought treatment for three transgenic lines and this enhanced water use efficiency was due to greater shoot DW accumulation (Table 10).

TABLE-US-00010 TABLE 10 Water use efficiency between day 0 and 4 of the drought treatment in transgenic lines of 35S-HP-At(270)PK220. shoot DW WUE (g shoot/ accumulated (g) water lost (g) kg water) entry d 0-d 4 d 0 to d 4 d 0 to d 4 65-4 0.090 62.5 1.44 (+22 to 33%) 12-2 0.079 62.2 1.27 (+7 to 17%) 56-3 0.079 62.7 1.25 (+6 to 16%) null (65-1) 0.068 62.7 1.08 WT 0.073 61.9 1.18

Transgenic Lines of 35S-HP-At(270)PK220 in Arabidopsis had Lower Water Loss Relative to Shoot Biomass and Enhanced WUE Under Optimal Conditions.

[0168] Plants of 35S-HP-At(270)PK220 transgenic lines 65-7 and 59-5, WT Columbia, hwe116 mutant and its parent were grown (5 per 3'' pot) under optimal conditions in a growth chamber (22.degree. C., 18 hr light--200 uE, 60% relative humidity) until first flower (n=8 per entry, per harvest). At first flower all pots in the water limited group were watered with the same amount of water (to a pot weight of 120 g in first 4 days and to 130 g for last 3 days (as plants grew larger they required more water). Pots were weighed daily to determine daily water loss and plants were harvested on day 0 and day 7 of this treatment. Water use efficiency (WUE) was calculated from the ratio of shoot biomass accumulated to water lost. The results are shown in Table 11.

TABLE-US-00011 TABLE 11 Water Use Efficiency under optimal conditions shoot DW WUE (g shoot/ accumulated (g) water lost (g) kg water) entry d 0-d 4 d 0 to d 4 d 0 to d 4 59-5 0.514 223 3.31 (+4%) 65-4 0.671 276 2.43 (+9%) WT 0.517 232 2.23 hwe116 0.420 191 2.19 (5%) parent 0.421 202 2.08

The results show that under optimal water conditions the two transgenic lines and the mutant had enhanced water use efficiency. Growth Rates of the 35S-HP-At(270)PK220 Transgenic Arabidopsis were Greater than Those of Controls During Both Optimal and Water Limited Conditions.

[0169] Plants of 35S-HP-At(270)PK220 transgenic line 65-4 and WT Columbia were grown (5 per 3'' pot) under optimal conditions in a growth chamber (22.degree. C., 18 hr light--150 uE, 60% relative humidity) until first flower (n=8 per entry, per treatment and per harvest). At first flower all pots in the water limited group were watered with the same amount of water (to a pot weight of 95 g), and further watering was stopped for 2 days. It took 2 days for the water limited group of plants to reach about 30% of initial soil water content (about 55 g total pot weight), referred to as pre-treatment. At that time the water limited treatment was deemed to have started (day 0 of treatment) and plants were watered daily up to a total pot weight of 55 g for 3 days, and up to 65 g in the following 4 days (until day 7 of treatment). The optimal group was maintained under optimal conditions by watering the pots daily up to 100 g total pot weight in the 2 pre-treatment days, the first 3 days of treatment and then up to 130 g in the last 4 days of treatment (as plants grew larger they required more water). The daily water loss from the pots was measured for all the plants and plants in both groups were harvested on days 0, 1, 2, 3, 5, and 7 of treatment for shoot dry weight determinations. The water loss relative to the shoot biomass (drought tolerant phenotype) was calculated over the initial two days before the start of treatment, during the first 3 days of treatment and during the last 4 days of treatment. The results under both optimal (Table 12) and water limited (Table 13) conditions are shown. The transgenic line 65-4 lost less water relative to shoot biomass than WT in both optimal and water limited conditions. Under limited water conditions this is consistent with enhanced drought tolerance phenotype.

TABLE-US-00012 TABLE 12 Water loss in g/shoot DW in g under optimal conditions. Entry pre-treatment d 0-d 3 d 3-d 7 65-4 231 .+-. 9 162 .+-. 3 237 .+-. 5 WT 275 .+-. 8 178 .+-. 7 243 .+-. 6

TABLE-US-00013 TABLE 13 Water loss in g/shoot DW in g and Drought tolerance (as percentage of WT) under water limited conditions. pre-treatment d 0-d 3 d 3-d 7 (drought toler. (drought toler. (drought toler. Entry in % of WT) in % of WT) in % of WT) 65-4 174 .+-. 2 (108%) 83 .+-. 2 (115%) 153 .+-. 6 (113%) WT 189 .+-. 4 (100%) 97 .+-. 4 (100%) 175 .+-. 4 (100%)

[0170] Growth rates of the plants were calculated over the seven days of both treatments. The results showed that transgenic line 65-4 had larger plants (up to 24%) than the wild type throughout the treatment under both conditions. The growth rate (shoot dry weight accumulated per day over the 7 days of treatment) was slightly greater for the transgenic line under both optimal and water limited conditions (63.3 and 21.3 mg shoot/day, respectively) than that of WT control (58.3 and 20.4 mg shoot/day, respectively).

The Transgenic Line of 35S-HP-At(270)PK220 Arabidopsis and the Hwe116 Mutant Grow Better Under Limited Nitrogen Conditions than Controls.

[0171] The 35S-HP-At(270)PK220 transgenic line 65-5, its segregated null control (null 65-1) and wild-type (WT) plus the hwe116 mutant and its parent control were analyzed for growth characteristics of young seedling under optimal and limited nitrogen conditions. Nitrogen content refers to the available nitrogen for plant growth, including nitrate and ammonium sources. Seedlings were grown on agar plates (10 per plate and 5 plates per entry and per treatment) containing either optimal nutrients (including 20 mM nitrogen) or low (limiting to growth) nitrogen (optimal all nutrients except for nitrogen being 0.5 mM). Plates were placed in a growth chamber at 18 hr lights of 200 uE and 22.degree. C. Seedlings were grown for 14 days before being harvested for shoot biomass (8 seedlings) and chlorophyll determinations (2 seedlings). On optimal plates there were no differences in average seedling shoot biomass except for the hwe116 mutant, as shown before had slightly smaller seedling shoot DW (not significant). On low nitrogen the hwe116 mutant had significantly bigger seedling shoot DW and showed 30% less inhibition in growth as compared to its parent. The transgenic line 65-5 showed slightly greater shoot DW than controls and was 5% to 7% less inhibited in growth than the controls (Table 14).

TABLE-US-00014 TABLE 14 Effect of nitrogen on seedling shoot DW Average seedling shoot DW (mg) Optimal N (20 mM) Low N (0.5 mM) entry Mean Std Err Mean Std Err % of opt 65-5 5.3 0.1 2.9 0.1 56% WT 5.5 0.3 2.8 0.1 51% hwe116 4.8 0.2 3.8 0.3 80% parent 5.1 0.2 2.6 0.1 50%

[0172] The total chlorophyll content of seedling shoots grown under low N levels reflected the shoot DW results. Chlorophyll content is very closely linked to available N and one of the major symptoms of N-deficiency in plants is leaf chlorosis or bleaching. Table 15 shows that chlorophyll content of the transgenic line 65-5 and the mutant hwe116 was reduced less than that of the controls.

TABLE-US-00015 TABLE 15 Effects of nitrogen on seedling shoot total chlorophyll content seedling shoot chlorophyll content (ug/g) Optimal N (20 mM) Low N (0.5 mM) entry Mean Std Err Mean Std Err % of opt 65-5 902 35 244 22 27% WT 854 102 156 17 18% hwe116 1006 51 376 37 37% parent 836 59 208 47 25%

[0173] These results confirmed that the hwe116 mutant grew better on limited nitrogen and the transgenic line showed the same trends. Therefore, down-regulation of the PK220 gene in plants appears to result in increased nitrogen use efficiency (accumulation of more biomass per unit of available nitrogen).

The Transgenic Line of 35S-HP-At(270)PK220 Arabidopsis and the Hwe116 Mutant Germinate Faster and have Higher Rates of Germination in the Cold.

[0174] Germination under cold (10.degree. C.) conditions was assessed in the transgenic line 65-5 carrying the 35S-HP-At(270)PK220 construct relative to WT-control and that of the hwe116 mutant relative to its parental control on agar plates containing optimal growth media. Four plates per entry with 30 seeds each were prepared and placed in the chamber at 10.degree. C., 18 hr light (200 uE). Germination (emergence of the radicle) scored as a percentage of viable seeds, was noted twice daily for 5 days starting with day 5 from placing of seeds on plates (no germination before day 5). Once no further changes were observed in germination all plates were placed in a chamber at 22.degree. C. to check for viability of the seeds that had not germinated. All entries showed 98 to 100% seed viability, the hwe116 mutant had 94%. viabilty. The results of the germination assessment at 10.degree. C. (Table 16) indicate that the transgenic line 65-5 germinated sooner than it's WT-control. The hwe116 mutant had higher rates of germination in the cold than its parent control. These data, together with the evidence that the mutant grows better under cold conditions are indicative of a greater seed and seedling vigor under cold stress

TABLE-US-00016 TABLE 16 percentage germination of viable seeds at 10.degree. C. Hours @ 10.degree. C. % Viable entry #reps 114.5 121 139 145 163 169 188.5 212.5 235 241 Seed 65-5 4 15.1 32.8 75.7 80.7 90.0 90.8 90.8 92.5 93.3 94.2 99.2 WT 4 5.9 16.0 55.4 62.9 78.1 79.0 80.7 80.7 81.5 81.5 98.4 hwe116 4 15.9 28.4 67.5 81.4 94.2 98.0 99.0 99.0 100.0 100.0 94.0 Parent 4 6.7 26.7 72.5 77.5 85.0 85.0 85.9 85.9 85.9 85.9 100.0

Gas Exchange Measurements Support Higher WUE in Transgenic 35S-HP-At(270)PK220 Arabidopsis Under Optimal Conditions

[0175] Plants of two transgenic lines and WT were grown in four inch diameter pots (one per pot) under optimal conditions in a growth chamber at 18 hr light (200 uE), 22.degree. C., 60% RH. Eight days from first open flower gas exchange measurements were made on the youngest, fully developed leaf of 10 to 11 replicates per entry. Photosynthesis and transpiration rates were measured inside the growth chamber at the ambient growth light and temperature conditions and 400 ppm carbon dioxide using Li-6400 and Arabidopsis leaf cuvette. From the ratio of photosynthesis to transpiration instantaneous water use efficiency (WUE) was calculated. The results are shown in Table 17. The WUE in the transgenic lines was 11 and 18% greater than that of the WT. This data is consistent with the WUE measurements over a period of few days using the ratio of biomass accumulated to water lost in transpiration.

TABLE-US-00017 TABLE 17 Photosynthesis (umol carbon dioxide/m2/s), transpiration (mmol H2O/m2/s) and WUE measured under optimal growth conditions. Phots. Trans. WUE (umol/ Photos. (mmol/ Trans. (Photos/ WUE entry m2/s) (% WT) m2/s) (% WT) Trans) (% WT) 59-6 3.9 .+-. 0.2 105% 4.2 .+-. 0.5 95% 1.03 .+-. 0.11 118% 65-5 3.6 .+-. 0.2 97% 3.8 .+-. 0.4 86% 0.97 .+-. 0.13 111% WT 3.7 .+-. 0.2 4.4 .+-. 0.2 0.87 .+-. 0.05

Drought Tolerance of 35S-HP-At(270)PK220 Transgenic Arabidopsis Results in Seed Yield and Biomass Protection Following Drought Stress.

[0176] Plants of two transgenic lines and the WT were grown (5 per 3 inch pot containing equal amount of soil) under optimal conditions in a growth chamber (22 C, 18 hr light of 200 uE, 60% RH) until first open flower. At first flower the drought treatment was applied to half of the plants while the other half was maintained under optimal conditions until maturity. The drought treatment consisted of watering all the plants to the same saturated water level. Plants were then weighed daily to monitor water loss from the pots and their water content was equalized daily by watering all pots to the level of the heaviest pot. As a result the soil water content was declining and reached stress levels with plants wilting on day 4. Plants were maintained at that stress level for another 2 days and on day 6 all plants were re-watered and maintained under optimal conditions for the rest of their life cycle. At maturity both optimal and drought plants were harvested for seed and shoot biomass. The impact of drought stress on both seed yield and shoot biomass was determined by comparing the optimal and drought treated plants. The results are shown in Table 18. Under optimal conditions the seed yield and the final shoot biomass of the transgenic lines was 7 to 10% higher than that of the WT. Following the drought stress during flowering the reduction in seed yield and the shoot biomass were not as great in transgenic plants as in the WT, resulting in seed yield protection of 5-7% and shoot biomass protection of 4%. The protection was calculated as the difference between the transgenics and WT in seed yield or shoot biomass a percentage of optimal.

TABLE-US-00018 TABLE 18 Seed yield and final shoot biomass from optimal and drought stressed plants, n = 10 Seed yield- Seed yield- Shoot DW- drought % of Shoot DW- % of entry opt (g) opt (g) (g) opt drought (g) opt 59-6 1.29 .+-. 0.05 2.96 .+-. 0.13 1.06 .+-. 0.03 82% 2.37 .+-. 0.07 80% 65-5 1.27 .+-. 0.03 2.89 .+-. 0.08 1.01 .+-. 0.02 80% 2.32 .+-. 0.06 80% WT 1.18 .+-. 0.04 2.69 .+-. 0.10 0.89 .+-. 0.02 75% 2.04 .+-. 0.05 76%

Over-Expression of Wild Type AtPK220 in hwe116.2 Background can Restore the WT Phenotype

[0177] Transgenic plants of 35S-AtPK220 (in hwe116.2) were grown (5 per 3 inch pot) under optimal conditions in a growth chamber as described above until the first open flower. Drought treatment was applied by watering all plants to the same saturated level. Further watering was withheld. Plants were weighed daily to determine the daily water loss and all plants were harvested on day 4 of treatment by which time all plants showed wilting. The water loss relative to final shoot biomass was used to calculate drought tolerance where that of WT was assumed at 100%. The data are shown in Table 19. Three transgenic lines showed a reduction in drought tolerance from the mutant levels as indicated by increased water loss relative to shoot biomass. The three transgenic lines also flowered earlier than the mutant line and similar to the time that the WT lines flowered. These results support the conclusion that the AtPK220 gene mutation in hwe116.2 is responsible for the altered phenotypes observed and expression of a WT gene restore the WT characteristics of a mutant plant.

TABLE-US-00019 TABLE 19 Water loss relative to shoot biomass and drought tolerance, n = 8 Water lost in Drought tolerance entry Days to flower 3 d/shoot DW d 4 (% of WT) 28-4 20.9 .+-. 0.1 155.1 .+-. 3.1 111% 2-4 21.8 .+-. 0.1 164.7 .+-. 2.4 105% 7-11 21.6 .+-. 0.1 177.9 .+-. 4.4 97% hwe116.2 23.1 .+-. 0.2 134.9 .+-. 3.6 117% WT 20.8 .+-. 0.2 173.4 .+-. 5.1 100%

Down Regulation of AtPK220 with the AtPK220-Promoter (P.sub.PK) in Arabidopsis Results in Enhanced Drought Tolerance of Plants

[0178] Arabidopsis plants of P.sub.PK-HP-At(270)PK220 were grown (5 per 3 inch pot) under optimal conditions in a growth chamber as mentioned above until the first open flower. Drought treatment was applied then by watering all plants to the same saturated level. Further water was withheld. Plants were weighed daily to determine the daily water loss and all plants were harvested on day 4 of treatment (all plants were wilted). The water loss relative to final shoot biomass was used to calculate drought tolerance where that of WT was assumed at 100%. The results of this study are shown in Table 20.

TABLE-US-00020 TABLE 20 Water loss relative to shoot biomass and drought tolerance, n = 8 Water lost in Drought tolerance entry Days to flower 3 d/shootDW d 4 (% WT) 14-04 22 158 .+-. 5 116% 15-06 20 183 .+-. 8 104% 45-3 20 185 .+-. 9 103% WT 20 190 .+-. 9 100%

[0179] One of the transgenic lines, 14-04, showed significantly greater drought tolerance than the wild type control as indicated by lower water loss relative to shoot biomass. This result is supported by data from line 14-04 that showed nearly complete inhibition of PK220 gene expression. The expression of AtPK220 was reduced by nearly 96% in the roots compared to WT. These results indicate that down regulation of PK220 in the roots is sufficient to achieve significant drought tolerance phenotype and presumably enhanced water use efficiency.

Overexpression of Brassica napus PK220 in the Arabidopsis Hwe116 Mutant can Restore the WT Phenotype

[0180] Transgenic plants of 35S-BnPK220 (in hwe116) plus two null controls (segregated siblings of the transgenic lines without the transgene, therefore hwe116 mutant) were grown (5 per 3 inch pot) under optimal conditions in a growth chamber as mentioned above until the first open flower. Drought treatment was applied then by watering all plants to the same saturated level. Further water was withheld. Plants were weighed daily to determine the daily water loss and all plants were harvested on day 4 of treatment (all plants were wilted). The water loss relative to final shoot biomass was used to calculate drought tolerance where that of WT was assumed at 100%. The results of this study are shown in Table 21. The results indicate that 6 lines had a reduction of 8% or more in drought tolerance as compared to the nulls (the hwe116 mutant background) and therefore restoration towards the WT phenotype. This indicates that BnPK220 is functional and can work in the Arabidopsis.

TABLE-US-00021 TABLE 21 Water loss relative to shoot DW and drought tolerance, n = 8 Water lost in Drought tolerance entry 3 d/shoot DW d 4 (% of null) 106-11 148 .+-. 6 98% 67-6 150 .+-. 4 97% 51-6 152 .+-. 4 96% 5-1 152 .+-. 2 95% 74-12 157 .+-. 5 92% 38-7 160 .+-. 5 90% 70-2 161 .+-. 2 89% 97-3 164 .+-. 5 87% 31-6 165 .+-. 4 87% 93-8 172 .+-. 4 82% Null 38-10 146 .+-. 3 100% Null 90-7 135 .+-. 5 107%

Transgenic Brassica Lines Having a 35S-AtPK220L292F Construct Showed Drought Tolerance and Higher Water Use Efficiency

[0181] Down regulation of endogenous PK220 activity was demonstrated using a dominant negative strategy by expression of the mutant allele of the AtPK220 gene in Brassica napus. Three Brassica napus transgenic lines having the Arabidopsis mutant AtPK220L292F gene and one null control line (a segregated sibling of the transgenic line lacking the transgene) per line were grown in 4.5 inch diameter pots containing equal amounts of soilless mix (Sunshine Professional Organic Mix #7) under optimal conditions of 16 hr light (400 uE) and 22 C day/18 C night temperature. At the four leaf stage, two treatments were applied. In the optimal treatment plants were watered to saturation and pots were covered with plastic bags to prevent any water loss from the pots due to evaporation. These plants were weighed daily for 7 days to determine the water loss from the pots due to transpiration and the same amount of water was added back daily to each pot to maintain the plants under optimal water conditions. In the drought treatment all plants were watered to saturation levels. Pots were covered with plastic and were weighed daily. However, these pots were watered daily to the level of the heaviest pots. This treatment went for 7 days with the soil water content gradually reaching stress levels. Plants started to wilt by day 5. At the end of the 7 days both groups of plants were harvested for shoot biomass determinations.

[0182] Gas exchange measurements were done on drought treated plants of two transgenic lines plus their nulls on days 3 and 4 of the treatment. Photosynthesis and transpiration were measured on leaf 3 under steady state growth conditions of 400 uE light, 400 ppm carbon dioxide and 22 C using Li-6400. From the ratio of photosynthesis to transpiration, water use efficiency (WUE) was calculated. The drought treated plants were used to calculate the drought tolerance (as percentage of their nulls). This was done using the ratio of cumulative daily transpirational water loss between days 3 and 7, relative to the final shoot dry weight and normalizing it to the nulls (set at 100%).

[0183] The results in Table 22 indicate that transgenic lines had strong trends toward greater drought tolerance. This was a result of lower water loss relative to shoot dry weight, a phenotype present also under optimal conditions.

[0184] The gas exchange data (Table 23) showed that on both days 3 and 4 of the drought treatment the transgenic plants had slightly higher WUE than controls (4 to 16%).

[0185] Water use efficiency calculated from the ratio of photosynthesis to transpiration provides only a single point, instantaneous measurement rather than cumulative measurement over the period of treatment and as a result may be of lesser magnitude.

[0186] In conclusion, the data with transgenic 35S-AtPK220L292F Brassica plants indicate that water use efficiency technology is transferable to Brassica when using a AtPK220L292F gene from a heterologous species.

TABLE-US-00022 TABLE 22 Water loss between days 3 and 7 relative to final shoot dry weight under optimal and drought treatment. Drought tolerance (% of the appropriate null). n = 8 optimal - g drought - g water lost water lost d 3-7/g d 3-7/g Drought tolerance entry shootDW d 7 shootDW d 7 (% of null) Tr-05 172 .+-. 6 121 .+-. 5 109% Null-05 190 .+-. 4 133 .+-. 7 100% Tr-27 194 .+-. 6 134 .+-. 7 113% Null-27 205 .+-. 8 155 .+-. 11 100% Tr-09 171 .+-. 10 129 .+-. 3 113% Null-09 178 .+-. 17 149 .+-. 13 100%

TABLE-US-00023 TABLE 23 Photosynthesis (umol carbon dioxide/m2/s), Transpiration (mmol H2O/m2/s) and WUE (Photos/Trans on days 3 and 4 of drought treatment. n = 8 Photos Trans. WUE Photos. Trans. WUE entry D3 D3 D3 D4 D4 D4 Tr-05 13.4 .+-. 1.2 2.1 .+-. 0.2 6.6 .+-. 0.2 11.6 .+-. 1.3 1.9 .+-. 0.2 6.1 .+-. 0.4 (116% (104% of null) of null) Null-05 14.4 .+-. 1.1 2.5 .+-. 0.2 5.7 .+-. 0.2 12.6 .+-. 1.2 2.2 .+-. 0.2 5.9 .+-. 0.3 Tr-27 14.1 .+-. 0.7 2.4 .+-. 0.2 5.9 .+-. 0.3 11.4 .+-. 1.6 1.9 .+-. 0.3 6.2 .+-. 0.5 (105% (108% of null) of null) Null-27 14.1 .+-. 1.3 2.5 .+-. 0.1 5.6 .+-. 0.5 13.7 .+-. 1.0 2.4 .+-. 0.1 5.7 .+-. 0.4

TABLE-US-00024 SEQUENCE ID REFERENCE CHART SPECIES SEQ ID NO: REFERENCE ARABIDOPSIS THALIANA SEQ ID NO: 1 AtPK220 NT 1299 ARABIDOPSIS THALIANA SEQ ID NO: 2 AtPK220 AA 432 ARABIDOPSIS THALIANA SEQ ID NO: 3 AtPK220L292F NT 1299 ARABIDOPSIS THALIANA SEQ ID NO: 4 AtPK220L292F AA 432 ARABIDOPSIS THALIANA SEQ ID NO: 5 AtPK220L292F_partial NT 1160 ARABIDOPSIS THALIANA SEQ ID NO: 6 AtPK220L292F_partial_orf AA 383 ARABIDOPSIS THALIANA SEQ ID NO: 7 AtPK220_partial NT 1160 ARABIDOPSIS THALIANA SEQ ID NO: 8 AtPK220_partial_orf AA 383 ARABIDOPSIS THALIANA SEQ ID NO: 9 AtPK220_with_UTR NT 1542 ARABIDOPSIS THALIANA SEQ ID NO: 10 AtPK220_for_35s-AtPK220 NT 1309 ARABIDOPSIS THALIANA SEQ ID NO: 11 AtPK220_partial NT 1177 ARABIDOPSIS THALIANA SEQ ID NO: 12 At(150)PK NT 154 ARABIDOPSIS THALIANA SEQ ID NO: 13 At(270)PK NT 288 ARABIDOPSIS THALIANA SEQ ID NO: 14 AtPK220_promoter NT 1510 ARABIDOPSIS THALIANA SEQ ID NO: 15 At4g32000_UTR NT 157 ARABIDOPSIS THALIANA SEQ ID NO: 16 At4g32000 NT 1257 ARABIDOPSIS THALIANA SEQ ID NO: 17 At4g32000 AA 418 ARABIDOPSIS THALIANA SEQ ID NO: 18 At5g11020 NT 1302 ARABIDOPSIS THALIANA SEQ ID NO: 19 At5g11020 AA 433 ARABIDOPSIS THALIANA SEQ ID NO: 20 At2g25440 NT 2016 ARABIDOPSIS THALIANA SEQ ID NO: 21 At2g25440 AA 671 ARABIDOPSIS THALIANA SEQ ID NO: 22 At2g23890 NT 1662 ARABIDOPSIS THALIANA SEQ ID NO: 23 At2g23890 AA 553 BRACHYPODIUM SEQ ID NO: 24 BdPK220 NT 1386 DISTACHYON BRASSICA NAPUS SEQ ID NO: 25 BnPK220 NT 1302 BRASSICA NAPUS SEQ ID NO: 26 BnPK220 AA 433 CICHORIUM ENDIVIA SEQ ID NO: 27 EL362007.1 NT 657 CICHORIUM ENDIVIA SEQ ID NO: 28 EL362007.1_ORF AA 218 CITRUS CLEMENTINA SEQ ID NO: 29 CX290402.1 NT 474 CITRUS CLEMENTINA SEQ ID NO: 30 CX290402.1_ORF AA 157 CITRUS SINENSIS SEQ ID NO: 31 CK934154.1 NT 770 CITRUS SINENSIS SEQ ID NO: 32 CK934154.1_ORF AA 257 COFFEA CANEPHORA SEQ ID NO: 33 DV708241.1 NT 621 COFFEA CANEPHORA SEQ ID NO: 34 DV708241.1_ORF AA 206 EUCALYPTUS GUNNII SEQ ID NO: 35 CT986101.1 NT 411 EUCALYPTUS GUNNII SEQ ID NO: 36 CT986101.1_ORF AA 136 FESTUCA ARUNDINACEA SEQ ID NO: 37 DT714073 NT 522 FESTUCA ARUNDINACEA SEQ ID NO: 38 DT714073_ORF AA 173 GINKGO BILOBA SEQ ID NO: 39 EX942240.1 NT 740 GINKGO BILOBA SEQ ID NO: 40 EX942240.1_ORF AA 247 GLYCINE MAX SEQ ID NO: 41 GmPK220 NT 1254 GLYCINE MAX SEQ ID NO: 42 GmPK220 AA 418 HELIANTHUS SEQ ID NO: 43 EE622910.1 NT 702 ARGOPHYLLUS HELIANTHUS SEQ ID NO: 44 EE622910.1_ORF AA 233 ARGOPHYLLUS HELIANTHUS CILIARIS SEQ ID NO: 45 EL429543.1 NT 752 HELIANTHUS CILIARIS SEQ ID NO: 46 EL429543.1_ORF AA 251 HELIANTHUS EXILIS SEQ ID NO: 47 EE654885.1 NT 630 HELIANTHUS EXILIS SEQ ID NO: 48 EE654885.1_ORF AA 209 HORDEUM VULGARE SEQ ID NO: 49 TC151622 NT 780 HORDEUM VULGARE SEQ ID NO: 50 TC151622_ORF AA 259 IPOMOEA BATATAS SEQ ID NO: 51 EE883089.1 NT 816 IPOMOEA BATATAS SEQ ID NO: 52 EE883089.1_ORF AA 272 LACTUCA SATIVA SEQ ID NO: 53 DW125133.1 NT 867 LACTUCA SATIVA SEQ ID NO: 54 DW125133.1_ORF AA 288 MEDICAGO TRUNCATULA SEQ ID NO: 55 Contig NT 804 MEDICAGO TRUNCATULA SEQ ID NO: 56 Contig AA 267 NICOTIANA TABACUM SEQ ID NO: 57 BP131484.1 NT 636 NICOTIANA TABACUM SEQ ID NO: 58 BP131484.1 AA 211 ORYZA SATIVA SEQ ID NO: 59 NM_001061720.1 NT 1437 ORYZA SATIVA SEQ ID NO: 60 NP_001055185.1 AA 478 PHYSCOMITRELLA SEQ ID NO: 61 EDQ75046.1_cds NT 891 PHYSCOMITRELLA SEQ ID NO: 62 EDQ75046.1 AA 297 PICEA SEQ ID NO: 63 TC12392 NT 1065 PICEA SEQ ID NO: 64 TC12392_orf AA 354 PINUS SEQ ID NO: 65 CT578985.1 NT 596 PINUS SEQ ID NO: 66 CT578985.1_ORF AA 199 POPULUS SEQ ID NO: 67 TC76879 NT 1377 POPULUS SEQ ID NO: 68 TC76879_ORF AA 459 SACCHARUM SEQ ID NO: 69 TC46535 NT 693 OFFICINARUM SACCHARUM SEQ ID NO: 70 TC46535_ORF AA 230 OFFICINARUM TRIPHYSARIA SEQ ID NO: 71 DR169688.1 NT 414 VERSICOLOR TRIPHYSARIA SEQ ID NO: 72 DR169688.1_ORF AA 137 VERSICOLOR TRITICUM AESTIVUM SEQ ID NO: 73 TC254793 NT 1140 TRITICUM AESTIVUM SEQ ID NO: 74 TC254793_ORF AA 380 VITIS VINIFERA SEQ ID NO: 75 CAO44295.1_cds NT 978 VITIS VINIFERA SEQ ID NO: 76 CAO44295.1 AA 325 ZEA MAYS SEQ ID NO: 77 TC333547 NT 1377 ZEA MAYS SEQ ID NO: 78 TC333547_ORF AA 458 ZEA MAYS SEQ ID NO: 79 ZmPK220 NT 1188 ZEA MAYS SEQ ID NO: 80 ZmPK220 AA 396 GOSSYPIUM SEQ ID NO: 81 TC79117 NT 1086 GOSSYPIUM SEQ ID NO: 82 TC79117_ORF AA 361 SOLANUM SEQ ID NO: 83 Contig3 NT 1089 LYCOPERSICUM AQUILEGIA SEQ ID NO: 84 DR918821 NT 875 AQUILEGIA SEQ ID NO: 85 DR918821_ORF AA 292 CENTAUREA MACULOSA SEQ ID NO: 86 EL933228.1 NT 696 CENTAUREA MACULOSA SEQ ID NO: 87 EL933228.1_ORF AA 231 CICHORIUM INTYBUS SEQ ID NO: 88 EH693146.1 NT 842 CICHORIUM INTYBUS SEQ ID NO: 89 EH693146.1_ORF AA 281 CUCUMIS MELO SEQ ID NO: 90 AM742189.1 NT 495 CUCUMIS MELO SEQ ID NO: 91 AM742189.1_ORF AA 164 ERAGROSTIS CURVULA SEQ ID NO: 92 EH186232.1 NT 375 ERAGROSTIS CURVULA SEQ ID NO: 93 EH186232.1_ORF AA 124 GERBERA HYBRID SEQ ID NO: 94 AJ753651.1 NT 414 GERBERA HYBRID SEQ ID NO: 95 AJ753651.1_ORF AA 137 HELIANTHUS PARADOXUS SEQ ID NO: 96 EL488199.1 NT 498 HELIANTHUS PARADOXUS SEQ ID NO: 97 EL488199.1_ORF AA 165 IPOMOEA NIL SEQ ID NO: 98 BJ566706.1 NT 612 IPOMOEA NIL SEQ ID NO: 99 BJ566706.1_ORF AA 203 NUPHAR ADVENA SEQ ID NO: 100 DT603238.1 NT 708 NUPHAR ADVENA SEQ ID NO: 101 DT603238.1_ORF AA 235 SYNTHETIC PRIMER SEQ ID NO: 102 747F NT 30 SYNTHETIC PRIMER SEQ ID NO: 103 747R NT 34 SYNTHETIC PRIMER SEQ ID NO: 104 C747F2 NT 32 SYNTHETIC PRIMER SEQ ID NO: 105 C747R2 NT 31 SYNTHETIC PRIMER SEQ ID NO: 106 A220BamF1 NT 42 SYNTHETIC PRIMER SEQ ID NO: 107 A220PstR NT 40 SYNTHETIC PRIMER SEQ ID NO: 108 K188R NT 30 SYNTHETIC PRIMER SEQ ID NO: 109 A220A1SmaF2 NT 53 SYNTHETIC PRIMER SEQ ID NO: 110 A220BamR NT 38 SYNTHETIC PRIMER SEQ ID NO: 111 A220SmaF NT 41 SYNTHETIC PRIMER SEQ ID NO: 112 A220BamF2 NT 41 SYNTHETIC PRIMER SEQ ID NO: 113 A220XbaR NT 39 SYNTHETIC PRIMER SEQ ID NO: 114 K116SacF NT 35 SYNTHETIC PRIMER SEQ ID NO: 115 K270SacR NT 37 SYNTHETIC PRIMER SEQ ID NO: 116 K116BamF NT 37 SYNTHETIC PRIMER SEQ ID NO: 117 K270XbaR NT 40 SYNTHETIC PRIMER SEQ ID NO: 118 PK81A1XbaF NT 52

SYNTHETIC PRIMER SEQ ID NO: 119 K81PmBamF NT 47 SYNTHETIC PRIMER SEQ ID NO: 120 Pm81SmaR2 NT 41 ARABIDOPSIS THALIANA SEQ ID NO: 121 AtPK220L292F_with_UTR NT 1309 SYNTHETIC PRIMER SEQ ID NO: 122 Bn81F NT 25 SYNTHETIC PRIMER SEQ ID NO: 123 Bn81R NT 32 SYNTHETIC PRIMER SEQ ID NO: 124 Bn81RAF1 NT 32 SYNTHETIC PRIMER SEQ ID NO: 125 Bn81RAF2 NT 32 SYNTHETIC PRIMER SEQ ID NO: 126 Bn81RAR1 NT 31 SYNTHETIC PRIMER SEQ ID NO: 127 Bn81RAR2 NT 37 SYNTHETIC PRIMER SEQ ID NO: 128 Bn81F1 NT 28 SYNTHETIC PRIMER SEQ ID NO: 129 Bn81R1 NT 27 SYNTHETIC PRIMER SEQ ID NO: 130 Gm81RAR1 NT 27 SYNTHETIC PRIMER SEQ ID NO: 131 Gm81RAR2 NT 29 SYNTHETIC PRIMER SEQ ID NO: 132 Cn81RAR1 NT 29 SYNTHETIC PRIMER SEQ ID NO: 133 Cn81RAR2 NT 28 SYNTHETIC PRIMER SEQ ID NO: 134 A220SacF NT 41 SYNTHETIC PRIMER SEQ ID NO: 135 Pm81NheF NT 47 SYNTHETIC PRIMER SEQ ID NO: 136 Pm81NheR NT 43 SYNTHETIC PRIMER SEQ ID NO: 137 Gm81RAF1 NT 29 SYNTHETIC PRIMER SEQ ID NO: 138 Gm81RAF2 NT 31 SYNTHETIC PRIMER SEQ ID NO: 139 Zm81RAF1 NT 31 SYNTHETIC PRIMER SEQ ID NO: 140 Zm81RAF2 NT 27 SYNTHETIC PRIMER SEQ ID NO: 141 MiR319XbaF NT 31 SYNTHETIC PRIMER SEQ ID NO: 142 MiR319BamR NT 33 SYNTHETIC PRIMER SEQ ID NO: 143 MiPK220F1 NT 40 SYNTHETIC PRIMER SEQ ID NO: 144 MiPK220R1 NT 35 SYNTHETIC PRIMER SEQ ID NO: 145 MiPK220F2 NT 35 SYNTHETIC PRIMER SEQ ID NO: 146 MiPK220R2 NT 42 ARTIFICIAL SEQUENCE SEQ ID NO: 147 Synthesized_gene_fragment NT 21 ARABIDOPSIS THALIANA SEQ ID NO: 148 At4g23713_w_genomic NT 399 ARTIFICIAL SEQUENCE SEQ ID NO: 149 Artificial_micro_RNA_construct NT 399 ARABIDOPSIS THALIANA SEQ ID NO: 150 Promoter At2g44790 NT 1475 SYNTHETIC PRIMER SEQ ID NO: 151 P790-H3-F NT 31 SYNTHETIC PRIMER SEQ ID NO: 152 P790-Xb-R NT 31 BRASSICA NAPUS SEQ ID NO: 153 BnPK220 NT 338 SYNTHETIC PRIMER SEQ ID NO: 154 Bn340BamF NT 38 SYNTHETIC PRIMER SEQ ID NO: 155 Bn340XbaR NT 37 SYNTHETIC PRIMER SEQ ID NO: 156 Bn340SacF NT 38 SYNTHETIC PRIMER SEQ ID NO: 157 Bn340SacR NT 37 SYNTHETIC PRIMER SEQ ID NO: 158 bWET XbaI F NT 24 SYNTHETIC PRIMER SEQ ID NO: 159 bWET BamHI R NT 28 SYNTHETIC PRIMER SEQ ID NO: 160 bWET ClaI R NT 28 BRACHYPODIUM SEQ ID NO: 161 BdPK220 NT 272 DISTACHYON SYNTHETIC PRIMER SEQ ID NO: 162 bWx BamHIF NT 31 SYNTHETIC PRIMER SEQ ID NO: 163 bWx ClaI R NT 30 BRACHYPODIUM SEQ ID NO: 164 BdWx intron 1 NT 1174 DISTACHYON SYNTHETIC PRIMER SEQ ID NO: 165 bWET BamHI end2 NT 22 SYNTHETIC PRIMER SEQ ID NO: 166 BdUBQ PvuI F NT 30 SYNTHETIC PRIMER SEQ ID NO: 167 BdUBQT PacI R NT 28 PANICUM VIRGATUM SEQ ID NO: 168 Pv(251)PK220 NT 251 SYNTHETIC PRIMER SEQ ID NO: 169 PvWET XbaI F NT 27 SYNTHETIC PRIMER SEQ ID NO: 170 PvWET BamHI R NT 27 SYNTHETIC PRIMER SEQ ID NO: 171 PvWET ClaI R NT 27 SYNTHETIC PRIMER SEQ ID NO: 172 PvWET BamHI end1 NT 25 SYNTHETIC PRIMER SEQ ID NO: 173 PvWET BamHI end2 NT 21 SORGHUN BICOLOR SEQ ID NO: 174 Sb(261)PK220 NT 261 SYNTHETIC PRIMER SEQ ID NO: 175 SbWET XbaI F NT 26 SYNTHETIC PRIMER SEQ ID NO: 176 SbWET BamHI R NT 28 SYNTHETIC PRIMER SEQ ID NO: 177 SbWET ClaI R NT 28 SORGHUN BICOLOR SEQ ID NO: 178 SbWx intron 1 NT 273 SYNTHETIC PRIMER SEQ ID NO: 179 SbWx BamHI NT 30 SYNTHETIC PRIMER SEQ ID NO: 180 SbWx ClaI R NT 34 SYNTHETIC PRIMER SEQ ID NO: 181 SbWET BamHI end1 NT 24 SYNTHETIC PRIMER SEQ ID NO: 182 SbWET BamHI end2 NT 21 SORGHUN BICOLOR SEQ ID NO: 183 SbGOS2 promoter NT 1000 SYNTHETIC PRIMER SEQ ID NO: 184 SbGOS2 HindIII F NT 28 SYNTHETIC PRIMER SEQ ID NO: 185 SbGOS2 HindIII R NT 30 SORGHUN BICOLOR SEQ ID NO: 186 SbUBQ promoter NT 1000 SYNTHETIC PRIMER SEQ ID NO: 187 SbUBQ PstI F NT 26 SYNTHETIC PRIMER SEQ ID NO: 188 SbUBQ PstI R NT 28 SORGHUN BICOLOR SEQ ID NO: 189 SbUBQ terminator NT 239 SYNTHETIC PRIMER SEQ ID NO: 190 SbUBQT KpnI F NT 27 SYNTHETIC PRIMER SEQ ID NO: 191 SbUBQT KpnI R NT 27 SYNTHETIC PRIMER SEQ ID NO: 192 SbUBQ PvuI F NT 34 BRASSICA NAPUS SEQ ID NO: 193 BnPK220 NT 1302 BRASSICA NAPUS SEQ ID NO: 194 BnPK220 AA 433 SYNTHETIC PRIMER SEQ ID NO: 195 Bd81RAR1 NT 31 SYNTHETIC PRIMER SEQ ID NO: 196 Bd81RAR2 NT 32 BRACHYPODIUM SEQ ID NO: 197 BdPK220 AA 461 DISTACHYON SYNTHETIC PRIMER SEQ ID NO: 198 A200A1AgeF NT 53 SYNTHETIC PRIMER SEQ ID NO: 199 A220AgeR NT 39 SYNTHETIC PRIMER SEQ ID NO: 200 bWET BamHI end1 NT 18 Sequences >SEQ ID NO: 1 ATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTCTCTAATTCTTTTGATGATCTTCATCACT GTCTCTGCTTCTTCTGCTTCAAATCCTTCTTTAGCTCCTGTTTACTCTTCCATGGCTACATTCTCTCCT CGAATCCAAATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTGATTGGTCTC ATAATCAGTTTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAAGAA CCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATGAGA CGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTCGAT ATCAAGACCCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGGTTT CGGATGCGTTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAACG TTAGCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTCGA ACGTTATATCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATGGA GAAAGGATCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATGCG TATGAAGATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCAGT TATCCACAGAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAGAT TTCGGTCTTGCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGACACTTGGT TATGTTGCCCCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTGGG GTAGTTCTGCTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCCAA TCTCTTGTAACTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATGCC GTTATAAAAGATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCGTG CAGCCAGAACCAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCCGG TAGAGCTAGGAGGGACTCTCCGGTTAACAAGATGA >SEQ ID NO: 2 MRELLLLLLLHFQSLILLMIFITVSASSASNPSLAPVYSSMATFSPRIQMGSGEEDRFDAHKKLLIGLIISFS SLGLIILFCFGFWVYRKNQSPKSINNSDSESGNSFSLLMRRLGSIKTQRRTSIQKGYVQFFDIKTLEKATG GFKESSVIGQGGFGCVYKGCLDNNVKAAVKKIENVSQEAKREFQNEVDLLSKIHHSNVISLLGSASEINS SFIVYELMEKGSLDEQLHGPSRGSALTWHMRMKIALDTARGLEYLHEHCRPPVIHRDLKSSNILLDSSFN AKISDFGLAVSLDEHGKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRPVEKLTPAQ CQSLVTWAMPQLTDRSKLPNIVDAVIKDTMDLKHLYQVAAMAVLCVQPEPSYRPLITDVLHSLVPLVP VELGGTLRLTR >SEQ ID NO: 3 ATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTCTCTAATTCTTTTGATGATCTTCATCACT GTCTCTGCTTCTTCTGCTTCAAATCCTTCTTTAGCTCCTGTTTACTCTTCCATGGCTACATTCTCTCCT CGAATCCAAATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTGATTGGTCTC ATAATCAGTTTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAAGAA CCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATGAGA CGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTCGAT ATCAAGACCCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGGTTT CGGATGCGTTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAACG TTAGCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTCGA ACGTTATATCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATGGA GAAAGGATCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATGCG TATGAAGATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCAGT TATCCACAGAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAGAT TTCGGTTTTGCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGACACTTGGTT ATGTTGCCCCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTGGGG TAGTTCTGCTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCCAATC TCTTGTAACTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATGCCGTT ATAAAAGATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCGTGCAG CCAGAACCAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCCGGTAG AGCTAGGAGGGACTCTCCGGTTAACAAGATGA >SEQ ID NO: 4 MRELLLLLLLHFQSLILLMIFITVSASSASNPSLAPVYSSMATFSPRIQMGSGEEDRFDAHKKLLIGLIISFS SLGLIILFCFGFWVYRKNQSPKSINNSDSESGNSFSLLMRRLGSIKTQRRTSIQKGYVQFFDIKTLEKATG GFKESSVIGQGGFGCVYKGCLDNNVKAAVKKIENVSQEAKREFQNEVDLLSKIHHSNVISLLGSASEINS SFIVYELMEKGSLDEQLHGPSRGSALTWHMRMKIALDTARGLEYLHEHCRPPVIHRDLKSSNILLDSSFN AKISDFGFAVSLDEHGKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRPVEKLTPAQ CQSLVTWAMPQLTDRSKLPNIVDAVIKDTMDLKHLYQVAAMAVLCVQPEPSYRPLITDVLHSLVPLVP VELGGTLRLTR >SEQ ID NO: 5 ATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTGATTGGTCTCATAATCAGT TTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAAGAACCAATCTCC AAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATGAGACGACTTGG CTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTCGATATCAAGAC CCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGGTTTCGGATGCG TTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAACGTTAGCCAA GAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTCGAACGTTATA TCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATGGAGAAAGGA TCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATGCGTATGAAG ATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCAGTTATCCACA GAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAGATTTCGGTTTT GCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGACACTTGGTTATGTTGCC CCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTGGGGTAGTTCTG CTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCCAATCTCTTGTAA CTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATGCCGTTATAAAAG ATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCGTGCAGCCAGAAC CAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCCGGTAGAGCTAGG AGGGACTCTCCGGTTAACAAGATGATTCACAGA

>SEQ ID NO: 6 MGSGEEDRFDAHKKLLIGLIISFSSLGLIILFCFGFWVYRKNQSPKSINNSDSESGNSFSLLMRRLGSIKTQ RRTSIQKGYVQFFDIKTLEKATGGFKESSVIGQGGFGCVYKGCLDNNVKAAVKKIENVSQEAKREFQNE VDLLSKIHHSNVISLLGSASEINSSFIVYELMEKGSLDEQLHGPSRGSALTWHMRMKIALDTARGLEYLH EHCRPPVIHRDLKSSNILLDSSFNAKISDFGFAVSLDEHGKNNIKLSGTLGYVAPEYLLDGKLTDKSDVY AFGVVLLELLLGRRPVEKLTPAQCQSLVTWAMPQLTDRSKLPNIVDAVIKDTMDLKHLYQVAAMAVL CVQPEPSYRPLITDVLHSLVPLVPVELGGTLRLTR >SEQ ID NO: 7 ATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTGATTGGTCTCATAATCAGT TTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAAGAACCAATCTCC AAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATGAGACGACTTGG CTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTCGATATCAAGAC CCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGGTTTCGGATGCG TTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAACGTTAGCCAA GAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTCGAACGTTATA TCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATGGAGAAAGGA TCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATGCGTATGAAG ATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCAGTTATCCACA GAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAGATTTCGGTCTT GCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGACACTTGGTTATGTTGCC CCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTGGGGTAGTTCTG CTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCCAATCTCTTGTAA CTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATGCCGTTATAAAAG ATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCGTGCAGCCAGAAC CAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCCGGTAGAGCTAGG AGGGACTCTCCGGTTAACAAGATGATTCACAGA >SEQ ID NO: 8 MGSGEEDRFDAHKKLLIGLIISFSSLGLIILFCFGFWVYRKNQSPKSINNSDSESGNSFSLLMRRLGSIKTQ RRTSIQKGYVQFFDIKTLEKATGGFKESSVIGQGGFGCVYKGCLDNNVKAAVKKIENVSQEAKREFQNE VDLLSKIHHSNVISLLGSASEINSSFIVYELMEKGSLDEQLHGPSRGSALTWHMRMKIALDTARGLEYLH EHCRPPVIHRDLKSSNILLDSSFNAKISDFGLAVSLDEHGKNNIKLSGTLGYVAPEYLLDGKLTDKSDVY AFGVVLLELLLGRRPVEKLTPAQCQSLVTWAMPQLTDRSKLPNIVDAVIKDTMDLKHLYQVAAMAVL CVQPEPSYRPLITDVLHSLVPLVPVELGGTLRLTR >SEQ ID NO: 9 ATCAAAAACTTTTCTTTTCTTAGCAAAAAAAACAAAAAAATGAGAGAGCTTCTTCTTCTTCTTCTTC TTCATTTTCAGTCTCTAATTCTTTTGATGATCTTCATCACTGTCTCTGCTTCTTCTGCTTCAAATCCTT CTTTAGCTCCTGTTTACTCTTCCATGGCTACATTCTCTCCTCGAATCCAAATGGGAAGTGGTGAAGA AGATAGATTTGATGCTCATAAGAAACTTCTGATTGGTCTCATAATCAGTTTCTCTTCTCTTGGCCTT ATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAAGAACCAATCTCCAAAATCCATCAACAACT CAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATGAGACGACTTGGCTCGATTAAAACTCAGA GAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTCGATATCAAGACCCTCGAGAAAGCGACAG GCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGGTTTCGGATGCGTTTACAAGGGTTGTTTGG ACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAACGTTAGCCAAGAAGCAAAACGAGAATTT CAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTCGAACGTTATATCATTGTTGGGCTCTGCA AGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATGGAGAAAGGATCATTAGATGAACAGTTA CATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATGCGTATGAAGATTGCTCTTGATACAGCT AGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCAGTTATCCACAGAGATTTGAAATCTTCG AATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAGATTTCGGTCTTGCTGTATCGCTGGATGA ACATGGCAAGAACAACATTAAACTCTCTGGGACACTTGGTTATGTTGCCCCGGAATACCTCCTTGA CGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTGGGGTAGTTCTGCTTGAACTCTTGTTGGG TAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCCAATCTCTTGTAACTTGGGCAATGCCACA ACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATGCCGTTATAAAAGATACAATGGATCTCAA ACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCGTGCAGCCAGAACCAAGTTACCGGCCGTT GATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCCGGTAGAGCTAGGAGGGACTCTCCGGTTA ACAAGATGATTCACAGAAACACGCCAAAAGAAATCCAAAGCCATTTAGATGATTTTCTTTTATCCT TTGCCTTTATATTTTTTTGTATAGGGTTATGATCCACTCATCTGAAAGTTTGGGGGTAAGAATGTGA GAATATAAGTTTTCAGGGTTGTTGAGTTCTATATAATTATATTTGTTTCTTTTTATTGTCAAATATAA TTATATTTTTGT >SEQ ID NO: 10 AAAATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTCTCTAATTCTTTTGATGATCTTCATC ACTGTCTCTGCTTCTTCTGCTTCAAATCCTTCTTTAGCTCCTGTTTACTCTTCCATGGCTACATTCTCT CCTCGAATCCAAATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTGATTGGT CTCATAATCAGTTTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAA GAACCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATG AGACGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTC GATATCAAGACCCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGG TTTCGGATGCGTTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAA CGTTAGCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTC GAACGTTATATCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATG GAGAAAGGATCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATG CGTATGAAGATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCA GTTATCCACAGAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAG ATTTCGGTCTTGCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGACACTTG GTTATGTTGCCCCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTG GGGTAGTTCTGCTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCC AATCTCTTGTAACTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATG CCGTTATAAAAGATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCG TGCAGCCAGAACCAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCC GGTAGAGCTAGGAGGGACTCTCCGGTTAACAAGATGATTCACAG >SEQ ID NO: 11 TCTGTGTCAGGAATCCAAATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTG ATTGGTCTCATAATCAGTTTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTAT CGCAAGAACCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTG TTAATGAGACGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAA TTTTTCGATATCAAGACCCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAA GGCGGTTTCGGATGCGTTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATC GAGAACGTTAGCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCA TCACTCGAACGTTATATCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAG CTTATGGAGAAAGGATCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGG CACATGCGTATGAAGATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGT CCACCAGTTATCCACAGAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGA TTTCAGATTTCGGTCTTGCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGA CACTTGGTTATGTTGCCCCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATG CATTTGGGGTAGTTCTGCTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCA ATGCCAATCTCTTGTAACTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGT GGATGCCGTTATAAAAGATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTT GTGCGTGCAGCCAGAACCAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTG GTTCCGGTAGAGCTAGGAGGGACTCTCCGGTTAACAAGATGATTCACAG >SEQ ID NO: 12 TCGCAAGAACCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTT GTTAATGAGACGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCA ATTTTTCGATATCAAGACCCTC >SEQ ID NO: 13 TCTGTGTCAGGAATCCAAATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTG ATTGGTCTCATAATCAGTTTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTAT CGCAAGAACCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTG TTAATGAGACGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAA TTTTTCGATATCAAGACCCTC >SEQ ID NO: 14 TGTTAAAAGCGATTTATAATTTACACCGTTTTGGTGTATATTTCTATCTATCCTTTTACAAGACCTAT ATATGTTATGTTATGGTGGTGTACTATTTTAAGTGAGCGACATAGTATTTTCTTCATATAGCTAATT AATCAACAACAATTTCCCAACTTACAACTATTTGCGTACTTTAAACTTATATTGAAAGAGAACTAC AAAATTATTTTTTTGTACAAGAGAATTATGGTCTTCGGATCAATAATTTCTCTAGATATAATATGTA AAGCCAACCCTATAATTTGTAAAATCCATGATTTGATATAATTTTCTTTTAAAATTGTGAATTGGCA GACAAAAACAACATTACATTTTGATTTAAATTCATAACTTTGACTTGCTAAGGAAACACCATGATT CATTTTTTGTCATTTGTTACATCATCACTAGAAATATTTGATCTAACTTTATTATGATAATAGACTAC ATACTACATATGCAGTTACGATTTTAAATACTACATATTTAAGCGTGTTTAAACTGTAACCATATCA TATAAAATGACATATCTAAAAGTGATTTTCAATATTTTGATATGATATGTGTTGTAGCACGGATAAT GATCTAATTTTTAAGTAATAAGCTTGTTCATTACAAAAGAGAAGAAAGTAGTATTGGGCCATGATT ATGTAAGGACAAAATAGGAAGATGTGGAAGAAGCCATTCGAGGGTTTTATTACAAAAACAGAGTA TATAATTGGTCATAATGTTTTATTCACTTAATTTAACATTATTGCATTATATTTTCATGAACACATAT TTCTTTAACTAAAAATATACACATATTTCTTATTGTAGATGAAGTGAAAAGAACAATATTTGGGTTC ACATCTATGGGTGAATCCTTTTAATCACCCCCTAAAATAAAAAAGGTGCCATATTTCTATTTTTAGA GAAAGATATAGAGCACCATTGGAGTGGTTTTGCTCCAAATATAGAGTTTAGAGAAATATATAATAC ACCATTGGAGATGCTCTAAAATGAATTTATTTATTTATTTAGATGGAAGATTCTAATTGGTTAGAAA AAGAGGAAGTGAATAATAGGATTCACCTATAAGAGTGAACCCAAGTATTTTTAAGAGATAATGTGT AAAGTAAATAGATGGTCATTGTGTGAATTATGAATAGAACCATGGTTTTCCATTTTTAATTGCTTAA CATAGGGTAATCAACAATGGGGTTTAATATGTCAATAGACAATAGTAAAGAAAGTATTTGATCTAT CCCAAATCTTTCTTCGTTCGTTAGTTCATCACTTTCTTTCTTTTTGGTTATATTAATGGTAGAGAACT AAAAATTCAACTTTTTATTCAAAAGCTCCCTTTCTCTTTCCCTCCTTTATTTGCCATAAAAGTGATTT CAAGAAGACAGCGAGAGAGAAAGTGATAGTTCGTTCACTCTTCGCTTTCTCAAGAATTTCAAAACA CCAAAAAAGTCTTTAGATTGAATTTCATCAAAAACTTTTC >SEQ ID NO: 15 AGACAAGAAAAAAGGAAACAAAATTTTATGAAAGAGATCTCCATTAGAGAAAGAGAGAGCGAGA GAGAGATTAATCTTGGAAGAGCAATCTCACATTCTCACACTGCTCTTAGAAAATCTCTCTTTCACCA TTAAAAATCCCAAAGAGTCTGGAGAA >SEQ ID NO: 16 ATGGGAAAGATTCTTCATCTTCTTCTTCTTCTTCTTAAGGTCTCTGTTCTTGAATTCATCATTAGTGT TTCTGCTTTTACTTCACCTGCTTCACAGCCTTCTCTTTCTCCTGTTTACACTTCCATGGCTTCCTTTTC TCCAGGGATCCACATGGGCAAAGGCCAAGAACACAAGTTAGATGCACACAAGAAACTTCTAATCG CTCTCATAATCACCTCATCTTCTCTAGGACTAATACTTGTATCTTGTTTATGCTTTTGGGTTTATTGG TCTAAGAAATCTCCCAAAAACACCAAGAACTCAGGTGAGAGTAGGATTTCATTATCCAAGAAGGG CTTTGTGCAGTCCTTCGATTACAAGACACTAGAGAAAGCAACAGGCGGTTTCAAAGACGGTAATCT TATAGGACGAGGCGGGTTCGGAGATGTTTACAAGGCCTGTTTAGGCAACAACACTCTAGCAGCAGT CAAAAAGATCGAAAACGTTAGTCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGATTTGTTGA GCAAGATTCACCACCCGAACATCATCTCATTGTTTGGATATGGAAATGAACTCAGTTCGAGTTTTAT CGTCTACGAGCTGATGGAAAGCGGATCATTGGATACACAGTTACACGGACCTTCTCGGGGATCGGC TTTAACATGGCACATGCGGATGAAGATTGCTCTTGATACAGCAAGAGCTGTTGAGTATCTCCACGA GCGTTGTCGTCCTCCGGTTATCCACAGAGATCTTAAATCGTCAAATATTCTCCTTGATTCTTCCTTCA ACGCCAAGATTTCGGATTTTGGTCTTGCGGTAATGGTGGGGGCTCACGGCAAAAACAACATTAAAC TATCAGGAACACTTGGTTATGTTGCTCCAGAATATCTCCTAGATGGAAAATTGACGGATAAGAGTG ATGTTTATGCGTTTGGTGTGGTTTTACTTGAACTCTTGTTAGGAAGACGGCCGGTTGAGAAATTGAG TTCGGTTCAGTGTCAATCTCTTGTCACTTGGGCAATGCCCCAACTTACGGATAGATCAAAGCTTCCG AAAATCGTGGATCCGGTTATCAAAGATACAATGGATCATAAGCACTTATACCAGGTGGCAGCCGTG GCAGTGCTTTGTGTACAACCAGAACCGAGTTATCGACCGTTGATAACCGATGTTCTTCACTCACTAG TTCCATTGGTTCCGGTAGAGCTAGGAGGGACTCTCCGGTTAATACCATCATCGTCTTGA >SEQ ID NO: 17 MGKILHLLLLLLKVSVLEFIISVSAFTSPASQPSLSPVYTSMASFSPGIHMGKGQEHKLDAHKKLLIALIIT SSSLGLILVSCLCFWVYWSKKSPKNTKNSGESRISLSKKGFVQSFDYKTLEKATGGFKDGNLIGRGGFG DVYKACLGNNTLAAVKKIENVSQEAKREFQNEVDLLSKIHHPNIISLFGYGNELSSSFIVYELMESGSLD TQLHGPSRGSALTWHMRMKIALDTARAVEYLHERCRPPVIHRDLKSSNILLDSSFNAKISDFGLAVMVG AHGKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRPVEKLSSVQCQSLVTWAMPQL TDRSKLPKIVDPVIKDTMDHKHLYQVAAVAVLCVQPEPSYRPLITDVLHSLVPLVPVELGGTLRLIPSSS >SEQ ID NO: 18 ATGAAGCAAATTGTTATAACAGCTCTTGTTTTACTACAAGCTTATGTTCTTCATCAATCCACATGTG TTATGTCCCTTACTACACAAGAATCTCCTTCTCCTCAACCTTCTGCTTTCACTCCCGCCTTATCTCCT GATTATCAACAGAGAGAGAAGGAATTGCATAAACAAGAGAGTAACAACATGAGACTGGTTATTTC ACTAGCAGCTACATTTTCCTTAGTTGGTATAATCTTACTTTGCTCTCTGCTTTATTGGTTTTGCCATA GGAGAAGAAACCTCAAGAGCTCAGGTTGTGGGTGTAGTGGAATCACATTCTTGAATCGGTTTAGTC GCTCAAAAACATTAGACAAGAGAACTACAAAGCAGGGAACAGTGTCATTGATCGATTACAATATA CTAGAAGAAGGAACTAGTGGTTTCAAGGAGAGTAACATTTTGGGTCAAGGTGGATTTGGATGTGTA TATTCTGCCACATTAGAGAACAACATTTCAGCTGCGGTTAAGAAGCTAGACTGTGCCAATGAAGAT GCAGCAAAGGAATTTAAGAGTGAGGTTGAGATATTGAGTAAGCTCCAGCACCCGAATATAATATC CCTTTTGGGTTATAGCACGAATGATACTGCGAGATTCATTGTCTATGAGCTGATGCCAAACGTTTCT CTGGAATCTCATTTACACGGATCTTCTCAGGGTTCGGCGATCACATGGCCTATGAGGATGAAGATT GCTCTTGATGTAACAAGGGGATTAGAATATTTGCATGAACATTGTCATCCAGCAATCATTCACAGG GACTTGAAATCATCCAACATCTTATTAGATAGCAATTTCAATGCTAAGATTTCAGATTTTGGTCTAG CTGTTGTTGATGGGCCAAAGAACAAGAACCATAAACTTTCCGGGACAGTTGGCTACGTTGCACCAG AGTATCTTCTCAACGGCCAATTGACAGAAAAGAGCGACGTGTATGCTTTTGGAGTAGTGTTATTAG AGCTTTTACTCGGGAAAAAACCTGTGGAGAAACTAGCTCCCGGTGAATGCCAATCCATCATCACTT GGGCAATGCCTTATCTCACTGATAGAACCAAGTTACCAAGCGTCATAGATCCTGCGATTAAAGATA CGATGGACTTGAAACACCTTTACCAGGTAGCGGCAGTGGCGATTTTGTGCGTGCAGCCAGAACCGA GTTATAGACCGTTGATTACAGACGTCTTGCATTCTCTTATACCTTTGGTTCCAATGGAACTTGGTGG AACCTTAAAAACCATCAAATGTGCTTCAATGGATCACTGTTAA >SEQ ID NO: 19 MKQIVITALVLLQAYVLHQSTCVMSLTTQESPSPQPSAFTPALSPDYQQREKELHKQESNNMRLVISLA ATFSLVGIILLCSLLYWFCHRRRNLKSSGCGCSGITFLNRFSRSKTLDKRTTKQGTVSLIDYNILEEGTSGF KESNILGQGGFGCVYSATLENNISAAVKKLDCANEDAAKEFKSEVEILSKLQHPNIISLLGYSTNDTARFI VYELMPNVSLESHLHGSSQGSAITWPMRMKIALDVTRGLEYLHEHCHPAIIHRDLKSSNILLDSNFNAKI SDFGLAVVDGPKNKNHKLSGTVGYVAPEYLLNGQLTEKSDVYAFGVVLLELLLGKKPVEKLAPGECQ SIITWAMPYLTDRTKLPSVIDPAIKDTMDLKHLYQVAAVAILCVQPEPSYRPLITDVLHSLIPLVPMELGG TLKTIKCASMDHC >SEQ ID NO: 20 ATGAAGACTATGTCCAAATCGTCTTTGCGTTTGCATTTTCTCTCGCTACTCTTACTTTGTTGTGTCTC CCCTTCAAGCTTTGTCATTATAAGATTCATTACACATAATCATTTTGATGGTCTAGTACGTTGTCATC CCCACAAGTTTCAAGCCCTTACGCAGTTCAAGAACGAGTTTGATACCCGCCGTTGCAACCACAGTA ACTACTTTAATGGAATCTGGTGTGATAACTCCAAGGTGCGGTCACAAAGCTACGACTACGGGACTG TCTCAGTGGAACTCTCAAATCAAACAGTAGCCTCTTCCAGTTTCATCATCTTCGCTACCTTGATCTC TCTCACAACAACTTCACCTCCTCTTCCCTCCCTTCCGAGTTTGTTTCCCACTTTGCGGAATCTAACCA AGCTCACAGTTTTAGACCTTTCTCATAATCACTTCTCCGGAACTTTGAAGCCCAACAATAGCCTCTT TGAGTTACACCACCTTCGTTACCTTAATCTCGAGGTCAACAACTTCAGTTCCTCACTCCCTTCCGAG TTTGGCTATCTCAACAATTTACAGCACTGTGGCCTCAAAGAGTTCCCAAACATATTCAAGACCCTTA AAAAAATGGAGGCTATAGACGTATCCAACAATAGAATCAACGGGAAAATCCCTGAGTGGTTATGG AGCCTTCCTCTTCTTCATTTAGTGAATATTTTAAATAATTCTTTTGACGGTTTCGAAGGATCAACGG AAGTTTTAGTAAATTCATCGGTTCGGATATTACTTTTGGAGTCAAACAACTTTGAAGGAGCACTTCC TAGTCTACCACACTCTATCAACGCCTTCTCCGCGGGTCATAACAATTTCACTGGAGAGATACCTCTT TCAATCTGCACCAGAACCTCACTTGGTGTCCTTGATCTAAACTACAACAACCTCATTGGTCCGGTTT CTCAATGTTTGAGTAATGTCACGTTTGTAAATCTCCGGAAAAACAATTTGGAAGGAACTATTCCTG AGACTTTCATTGTCGGTTCCTCGATAAGGACACTTGATGTTGGATACAATCGACTAACGGGAAAGC TTCCAAGGTCTCTTTTGAACTGCTCATCTCTAGAGTTTCTAAGCGTTGACAACAACAGAATCAAAGA CACATTTCCTTTCTGGCTCAAGGCTTTACCAAAGTTACAAGTCCTTACCCTAAGTTCAAACAAGTTT TATGGTCCTATATCTCCTCCTCATCAAGGTCCTCTCGGGTTTCCAGAGCTGAGAATACTTGAGATAT CTGATAATAAGTTTACTGGAAGCTTGTCGTCAAGATACTTTGAGAATTGGAAAGCATCGTCCGCCA TGATGAATGAATATGTGGGTTTATATATGGTTTACGAGAAGAATCCTTATGGTGTAGTTGTCTATAC CTTTTTGGATCGTATAGATTTGAAATACAAAGGTCTAAACATGGAGCAAGCGAGGGTTCTCACTTC CTACAGCGCCATTGATTTTTCTAGAAATCTACTTGAAGGAAATATTCCTGAATCCATTGGACTTTTA AAGGCATTGATTGCACTAAACTTATCGAACAACGCTTTTACAGGCCATATTCCTCAGTCTTTGGCAA ATCTTAAGGAGCTCCAGTCACTAGACATGTCTAGGAACCAACTCTCAGGGACTATTCCTAATGGAC TCAAGCAACTCTCGTTTTTGGCTTACATAAGTGTGTCTCATAACCAACTCAAGGGTGAAATACCAC AAGGAACACAAATTACTGGGCAATTGAAATCTTCCTTTGAAGGGAATGTAGGACTTTGTGGTCTTC CTCTCGAGGAAAGGTGCTTCGACAATAGTGCATCTCCAACGCAGCACCACAAGCAAGACGAAGAA GAAGAAGAAGAACAAGTGTTACACTGGAAAGCGGTGGCAATGGGGTATGGACCTGGATTGTTGGT TGGATTTGCAATTGCATATGTCATTGCTTCATACAAGCCGGAGTGGCTAACCAAGATAATTGGTCC GAATAAGCGCAGAAACTAG >SEQ ID NO: 21 MKTMSKSSLRLHFLSLLLLCCVSPSSFVIIRFITHNHFDGLVRCHPHKFQALTQFKNEFDTRRCNHSNYF NGIWCDNSKVRSQSYDYGTVSVELSNQTVASSSFIIFATLISLTTTSPPLPSLPSLFPTLRNLTKLTVLDLS HNHFSGTLKPNNSLFELHHLRYLNLEVNNFSSSLPSEFGYLNNLQHCGLKEFPNIFKTLKKMEAIDVSNN RINGKIPEWLWSLPLLHLVNILNNSFDGFEGSTEVLVNSSVRILLLESNNFEGALPSLPHSINAFSAGHNN FTGEIPLSICTRTSLGVLDLNYNNLIGPVSQCLSNVTFVNLRKNNLEGTIPETFIVGSSIRTLDVGYNRLTG KLPRSLLNCSSLEFLSVDNNRIKDTFPFWLKALPKLQVLTLSSNKFYGPISPPHQGPLGFPELRILEISDNK FTGSLSSRYFENWKASSAMMNEYVGLYMVYEKNPYGVVVYTFLDRIDLKYKGLNMEQARVLTSYSAI DFSRNLLEGNIPESIGLLKALIALNLSNNAFTGHIPQSLANLKELQSLDMSRNQLSGTIPNGLKQLSFLAYI SVSHNQLKGEIPQGTQITGQLKSSFEGNVGLCGLPLEERCFDNSASPTQHHKQDEEEEEEQVLHWKAVA MGYGPGLLVGFAIAYVIASYKPEWLTKIIGPNKRRN

>SEQ ID NO: 22 ATGACTTCCTCTCGCCGTCTTCTTCTTCCTCTCGGAGCATCGCTCACTAGAGGAAGATTTTCTTCCGA TCAAATCCGAAATGGATTTCTAAGAAACTTCCGTGGATTCGCCACCGTAACTTCGTCGGAACCGGC CTTAGCCAATCTGGAAGCGAAATATGCCGTAGCGTTGCCAGAATGTTCAACAGTAGAGGACGAGA TCACGAAGATCCGTCATGAATTCGAGTTAGCGAAACAGAGGTTTCTTAATATCCCTGAAGCTATTA ATAGTATGCCGAAGATGAATCCTCAAGGGATATATGTGAATAAGAATCTGAGATTGGATAATATAC AAGTTTATGGATTTGATTATGATTACACTTTGGCACATTACTCTTCTCACTTACAGAGTTTGATCTAT GATCTTGCCAAGAAACATATGGTTAATGAGTTTAGATATCCTGATGTTTGCACTCAGTTTGAGTATG ATCCTACTTTTCCAATCCGTGGGTTGTACTATGATAAACTAAAAGGATGCCTCATGAAATTGGATTT CTTCGGTTCAATCGAGCCAGATGGGTGTTATTTTGGTCGTCGTAAGCTTAGTAGGAAGGAAATAGA AAGCATGTATGGAACGCGGCACATAGGTCGTGATCAAGCGAGAGGTTTGGTGGGATTGATGGATTT CTTCTGTTTTAGCGAGGCGTGTCTTATAGCAGACATGGTGCAATATTTTGTTGACGCCAAACTTGAG TTTGATGCCTCTAACATCTACAATGATGTCAATCGTGCTATTCAACATGTCCATAGAAGTGGATTGG TTCATAGAGGAATTCTTGCTGATCCCAACAGATATTTGCTAAAAAATGGTCAGCTTCTACGTTTCCT GAGAATGCTAAAAGATAAAGGAAAGAAGCTTTTTTTGCTGACCAACTCTCCGTATAATTTTGTTGA TGGCGGAATGCGCTTTCTAATGGAGGAATCTTTTGGCTTCGGAGATTCCTGGCGAGAACTCTTTGAT GTTGTGATTGCTAAAGCAAATAAACCAGAATTTTACACATCTGAGCACCCTTTCCGTTGTTATGATT CGGAGAGGGATAATTTGGCATTTACAAAAGTGGATGCATTTGACCCAAAGAAAGTTTATTATCATG GTTGTCTTAAATCCTTCCTTGAAATCACAAAGTGGCATGGCCCTGAGGTGATTTATTTCGGAGATCA CTTATTTAGTGATCTAAGAGGGCCTTCAAAAGCTGGTTGGCGAACTGCTGCCATAATTCATGAGCT CGAGCGAGAGATACAGATACAAAATGATGATAGCTACCGGTTTGAGCAGGCCAAGTTCCATATTAT CCAAGAGTTACTCGGTAGATTTCACGCGACTGTATCAAACAATCAGAGAAGTGAAGCATGCCAATC ACTTTTGGATGAGCTGAACAATGCGAGGCAGAGAGCAAGAGACACGATGAAACAAATGTTCAACA GATCGTTTGGAGCTACATTTGTCACAGACACTGGTCAAGAATCAGCATTCTCTTATCACATCCACCA ATACGCAGACGTTTATACCAGTAAACCTGAGAACTTTCTGTTATACCGACCTGAAGCCTGGCTTCA CGTTCCTTACGATATCAAGATCATGCCACATCATGTCAAGGTTGCTTCAACCCTTTTCAAAACCTGA >SEQ ID NO: 23 MTSSRRLLLPLGASLTRGRFSSDQIRNGFLRNFRGFATVTSSEPALANLEAKYAVALPECSTVEDEITKIR HEFELAKQRFLNIPEAINSMPKMNPQGIYVNKNLRLDNIQVYGFDYDYTLAHYSSHLQSLIYDLAKKHM VNEFRYPDVCTQFEYDPTFPIRGLYYDKLKGCLMKLDFFGSIEPDGCYFGRRKLSRKEIESMYGTRHIGR DQARGLVGLMDFFCFSEACLIADMVQYFVDAKLEFDASNIYNDVNRAIQHVHRSGLVHRGILADPNRY LLKNGQLLRFLRMLKDKGKKLFLLTNSPYNFVDGGMRFLMEESFGFGDSWRELFDVVIAKANKPEFYT SEHPFRCYDSERDNLAFTKVDAFDPKKVYYHGCLKSFLEITKWHGPEVIYFGDHLFSDLRGPSKAGWRT AAIIHELEREIQIQNDDSYRFEQAKFHIIQELLGRFHATVSNNQRSEACQSLLDELNNARQRARDTMKQM FNRSFGATFVTDTGQESAFSYHIHQYADVYTSKPENFLLYRPEAWLHVPYDIKIMPHHVKVASTLFKT >SEQ ID NO: 24 ATGGAGATTCCGGCGGCGCCGCCGCCTCCATTGCCGGTGCTGTGCTCGTACGTCGTCTTC TTGCTGCTGCTGTCTTCGTGCTCACTGGCCAGAGGGAGGATCGCGGTTTCTTCCCCGGGC CCGTCGCCTGTGGCCGCCGCCGTTACAGCCAATGAGACCGCTTCATCCTCTTCTTCTCCG GTGTTTCCGGCCGCTCCTCCCGTCGTGATCACAGTGGTGAGGCACCACCATTACCACCGG GAGCTGGTCATCTCCGCTGTCCTCGCCTGCGTCGCCACCGCCATGATCCTCCTCTCCACA CTCTACGCCTGGACGATGTGGCGGCGGTCTCGCCGGACCCCCCACGGCGGCAAGGGCCGC GGCCGGAGATCAGGGATCACACTGGTGCCAATCCTGAGCAAGTTCAATTCAGTGAAGATG AGCAGGAAGGGGGGCCTTGTGACGATGATCGAGTACCCGTCGCTGGAGGCGGCGACAGGC AAGTTCGGCGAGAGCAATGTGCTCGGTGTCGGCGGCTTCGGTTGCGTTTATAAGGCGGCG TTTGATGGCGGTGCCACCGCCGCCGTGAAGAGGCTTGAAGGCGGCGGGCCGGATTGCGAG AAGGAATTCGAGAATGAGCTGGATTTGCTTGGCAGGATCAGGCACCCAAACATAGTGTCT CTCCTGGGCTTCTGTGTCCATGGTGGCAATCACTACATTGTTTATGAGCTCATGGAGAAG GGATCATTGGAGACACAGCTGCATGGGTCTTCACATGGATCTGCTCTGAGCTGGCACGTT CGGATGAAGATCGCGCTCGATACGGCGAGGGGATTAGAGTATCTTCATGAGCACTGCAAT CCACCTGTGATCCATAGGGATCTGAAACCTTCTAATATACTTTTAGATTCAGACTTCAAT GCTAAGATTGCAGATTTTGGCCTTGCGGTCACCGGTGGGAATCTCAACAAAGGGAACCTG AAGCTTTCCGGGACCTTGGGTTATGTAGCCCCTGAGTACTTATTAGATGGGAAGTTGACT GAGAAGAGCGATGTATACGCATTTGGAGTAGTGCTTCTAGAGCTCCTGATGGGAAGGAAG CCTGTTGAGAAAATGTCACCATCTCAGTGCCAATCAATTGTGTCATGGGCTATGCCTCAG CTGACCGACAGATCGAAGCTCCCCAACATAATTGACCTGGTGATCAAGGACACCATGGAC CCAAAACACTTGTACCAAGTTGCAGCAGTGGCTGTTCTATGTGTGCAGCCCGAACCGAGC TACAGACCACTGATAACAGATGTTCTCCACTCTCTTGTTCCTCTAGTGCCTGCGGAGCTC GGAGGAACACTCAGGGTTGCAGAGCCACCTTCACCTTCTCCAGACCAAAGACATTATCCT TGTTGA >SEQ ID NO: 25 ATGAAGAAACTGGTTCATCTTCAGTTTTTGTTTCTTGTCAAGATCTTTGCTACTCAATTCCTCACTCC TTCTTCATCATCTTTTGCTGCTTCAAATCCTTCTATAGCTCCTGTTTACACCTCCATGACTACTTTCTC TCCAGGAATTCAAATGGGAAGTGGTGAAGAACACAGATTAGATGCACATAAGAAACTCCTGATTG GTCTTATAATCAGTTCCTCTTCTCTTGGTATCATAATCTTGATTTGCTTTGGCTTCTGGATGTACTGT CGCAAGAAAGCTCCCAAACCCATCAAGATTCCGGATGCCGAGAGTGGGACTTCATCATTTTCAATG TTTGTGAGGCGGCTAAGCTCAATTAAAACTCACAGAACATCTAGCAATCAGGGTTATGTGCAGCGT TTCGATTCCAAGACGCTAGAGAAAGCGACAGGCGGTTTCAAAGACAGTAATGTAATCGGACAGGG CGGTTTCGGATGCGTTTACAAGGCTTCTTTGGACAGCAACACTAAAGCAGCGGTTAAAAAGATCGA AAACGTTACCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGAGCTGTTGAGCAAGATCCAGC ACTCCAATATTATATCATTGTTGGGCTCTGCAAGTGAAATCAACTCGAGTTTCGTCGTTTATGAGTT GATGGAGAAAGGATCCTTAGATGATCAGTTACATGGACCTTCGTGTGGATCCGCTCTAACATGGCA TATGCGTATGAAGATTGCTCTAGATACAGCTAGAGGACTAGAGTATCTCCATGAACATTGTCGTCC ACCAGTTATCCACAGGGACCTGAAATCGTCTAATATTCTTCTTGATTCTTCCTTCAATGCCAAGATT TCAGATTTTGGTCTGGCTGTATCGGTTGGAGTGCATGGGAGTAACAACATTAAACTCTCTGGGACA CTTGGTTATGTTGCCCCGGAATATCTCCTAGACGGAAAGTTGACGGATAAGAGTGATGTCTATGCA TTTGGGGTGGTTCTTCTTGAACTTTTGTTGGGTAGGCGGCCGGTTGAGAAATTGAGTCCATCTCAGT GTCAATCTCTTGTGACTTGGGCAATGCCACAACTTACCGATAGATCGAAACTCCCAAACATCGTGG ATCCGGTTATAAAAGATACAATGGATCTTAAGCACTTATACCAAGTAGCAGCCATGGCTGTGCTGT GCGTACAGCCAGAACCGAGTTACCGGCCGCTGATAACCGATGTTCTTCATTCACTTGTTCCATTGGT TCCGGTAGAGCTAGGAGGGACTCTCCGGTTAACCCGATGA >SEQ ID NO: 26 MKKLVHLQFLFLVKIFATQFLTPSSSSFAASNPSIAPVYTSMTTFSPGIQMGSGEEHRLDAHKKLLIGLIIS SSSLGIIILICFGFWMYCRKKAPKPIKIPDAESGTSSFSMFVRRLSSIKTHRTSSNQGYVQRFDSKTLEKAT GGFKDSNVIGQGGFGCVYKASLDSNTKAAVKKIENVTQEAKREFQNEVELLSKIQHSNIISLLGSASEIN SSFVVYELMEKGSLDDQLHGPSCGSALTWHMRMKIALDTARGLEYLHEHCRPPVIHRDLKSSNILLDSS FNAKISDFGLAVSVGVHGSNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRPVEKLSP SQCQSLVTWAMPQLTDRSKLPNIVDPVIKDTMDLKHLYQVAAMAVLCVQPEPSYRPLITDVLHSLVPL VPVELGGTLRLTR >SEQ ID NO: 27 ATTTTTGGTGTTGAAATGATGCACAACGGATCTTTGGAATCCCAATTGCATGGTCCGTCTCATGGAA CTGGCTTAAGCTGGCAGCATCGAATGAAAATTGCACTTGATATTGCACGAGGACTAGAGTATCTTC ACGAGCGCTGTACCCCGCCTGTGATTCATAGAGATCTGAAATCGTCCAACATTCTTCTAGGTTCGA ACTACAATGCTAAACTTTCTGATTTCGGGCTCGCGATTACTGGTGGGATTCAGGGCAAGAACAACG TAAAGCTTTCGGGAACATTAGGTTATGTAGCTCCAGAATACCTCTTAGATGGTAAACTTACTGATA AAAGTGATGTTTATGCGTTTGGAGTTGTACTTCTTGAACTTTTGATAGGTAGAAAACCAGTGGAGA AAATGTCACCATCTCAATGCCAATCTATCGTTACATGGGCAATGCCTCAACTAACCGACCGATCAA AGCTTCCTAACATCGTTGATCCCGTGATTAGAGATACAATGGACTTGAAGCACTTGTATCAAGTTG CTGCGGTTGCTGTGCTATGTGTACAACCGGAACCGAGTTACAGGCCATTGATAACAGATGTTTTGC ATTCGTTCATCCCACTTGTACCTGTTGAGCTTGGAGGGTCGCTAAGAGTTACCGAATCTTGA >SEQ ID NO: 28 IFGVEMMHNGSLESQLHGPSHGTGLSWQHRMKIALDIARGLEYLHERCTPPVIHRDLKSSNILLGSNYN AKLSDFGLAITGGIQGKNNVKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLIGRKPVEKMSPSQ CQSIVTWAMPQLTDRSKLPNIVDPVIRDTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSFIPLVPVE LGGSLRVTES >SEQ ID NO: 29 AATTCGGCACGAGGGCTGGATTCCAGTTTTAATGCAAAGCTTTCAGATTTTGGCCTTTCTGTGACTG CTGGAACCCAGAGTAGGAATGTTAAGATCTCTGGAACTCTGGGTTATGTTGCCCCGGAGTACCTAT TAGAAGGAAAACTAACTGATAAAAGTGATGTATATGCTTTCGGAGTTGTATTGCTGGAACTTTTGA TGGGGAGAAGGCCTGTGGAAAAGATGTCACCAACTCAATGTCAATCAATGGTCACATGGGCCATG CCTCAGCTCACCGATAGATCAAAGCTTCCAAACATTGTGGATCCAGTAATTAGAGACACAATGGAT TTAAAGCACTTATACCAGGTAGCCGCTGTGGCAGTGCTATGTATACAACCTGAACCAAGTTATAGG CCATTGATAACCGACGTTCTGCATTCCCTCATTCCTCTTGTACCTACCGACCTTGGAGGGTCACTCC GAGTGACCTAA >SEQ ID NO: 30 NSARGLDSSFNAKLSDFGLSVTAGTQSRNVKISGTLGYVAPEYLLEGKLTDKSDVYAFGVVLLELLMG RRPVEKMSPTQCQSMVTWAMPQLTDRSKLPNIVDPVIRDTMDLKHLYQVAAVAVLCIQPEPSYRPLITD VLHSLIPLVPTDLGGSLRVT >SEQ ID NO: 31 GGATTGTGTTTGTGGCTTTATCATTTGAAGTACTCCTTCAAATCCAGTAACAAGAATGCAAAGAGC AAAGATTCTGAGAATGGAGTTGTGTTATCATCATTTTTGGGCAAATTCACTTCTGTGAGGATGGTTA GTAAGAAGGGATCTGCTATTTCATTTATTGAGTATAAGCTGTTAGAGAAAGCCACCGACAGTTTTC ATGAGAGTAATATATTGGGTGAGGGTGGATTTGGATGTGTTTACAAGGCTAAATTGGATGATAACT TGCACGTCGCTGTCAAAAAATTAGATTGTGCAACACAAGATGCCGGCAGAGAATTTGAGAATGAG GTGGATTTGCTGAGTAATATTCACCACCCAAATGTTGTTTGTCTGTTGGGTTATAGTGCTCATGATG ACACAAGGTTTATTGTTTATGAATTGATGGAAAATCGGTCCCTTGATATTCAATTGCATGGTCCTTC TCATGGATCAGCATTGACTTGGCATATGCGAATGAAAATTGCTCTTGATACCGCTAGAGGATTAGA ATATTTACATGAGCACTGCAACCCTGCAGTCATTCATAGAGATCTGAAATCCTCCAATATACTTCTA GATTCCAAGTTTAATGCTAAGCTCTCAGATTTTGGTCTTGCCATAACCGATGGATCCCAAAACAAG AACAATCTTAAGCTTTCGGGCACTTTGGGATATGTGGCTCCCGAGTATCTTTTAGATGGTAAATTGA CAGACAAGAGTGATGTCTATGCTTTTGGAGTTGTGCTTCT >SEQ ID NO: 32 GLCLWLYHLKYSFKSSNKNAKSKDSENGVVLSSFLGKFTSVRMVSKKGSAISFIEYKLLEKATDSFHES NILGEGGFGCVYKAKLDDNLHVAVKKLDCATQDAGREFENEVDLLSNIHHPNVVCLLGYSAHDDTRFI VYELMENRSLDIQLHGPSHGSALTWHMRMKIALDTARGLEYLHEHCNPAVIHRDLKSSNILLDSKFNA KLSDFGLAITDGSQNKNNLKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLL >SEQ ID NO: 33 GCATTGACATGGCATCTTAGGATGAAAATTGCCCTTGATGTAGCTAGAGGATTAGAATTTTTGCAT GAGCACTGCCACCCAGCAGTGATCCATAGAGATCTGAAATCATCTAATATCCTTCTGGATTCAAAT CTCAATGCTAAGCTATCTGATTTTGGTCTTGCCATTCTTGATGGGGCTCAAAATAAGAACAACATCA AGCTTTCTGGAACCTTGGGCTATGTAGCTCCAGAGTACCTCTTAGATGGTAAATTGACTGACAAGA GTGATGTTTATGCTTTTGGAGTGGTGCTTTTGGAGCTTCTCCTGAGAAGAAAGCCTGTGGAGAAGCT GGCACCAGCTCAATGCCAATCTATAGTCACATGGGCTATGCCTCAGCTGACAGATAGATCAAAGCT TCCAAACATCGTGGATCCTGTGATTAGAAATGCTATGGATATAAAGCACTTATTCCAGGTTGCTGC AGTCGCTGTGCTATGCGTGCAGCCTGAACCAAGCTATCGACCACTGATAACAGATGTGTTGCATTC CCTTGTTCCCCTTGTTCCTATGGAGCTTGGCGGGACGCTCAGAGTTGAACGACCTGCTTCTGTGACC TCTCTGTTGATTGATTCTACCTGA >SEQ ID NO: 34 ALTWHLRMKIALDVARGLEFLHEHCHPAVIHRDLKSSNILLDSNLNAKLSDFGLAILDGAQNKNNIKLS GTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLRRKPVEKLAPAQCQSIVTWAMPQLTDRSKLPNIV DPVIRNAMDIKHLFQVAAVAVLCVQPEPSYRPLITDVLHSLVPLVPMELGGTLRVERPASVTSLLIDST >SEQ ID NO: 35 ACTGAGGTGACCCGGAAGAAAAACAGGGTAAAGCTATCGGGCACTTTGGGTTATGTAGCCCCAGA ATATGTCTTGGATGGTAAATTGACTGATAAGAGTGATGTCTATGCCTTTGGAGTTGTGCTTTTGGAG CTCCTTTTGAGAAGAAGGCCTCTTGAGATAGTAGCACCCACTCAGTGCCAGTCTATTGTTACATGG GCCATGCCTCAGCTGACCGACCGAACTAAGCTTCCAGATATTGTGGATCCTGTAATTAGAGATGCG ATGGATGTCAAGCACTTATACCAGGCAGCTGCTGTTGCTGTTTTGTGTCTGCAACCAGAACCGATCT ACCGGCCACTGATAACGGATGTACTCCACTCTCTCATTCCACTTGTACCCGTTGAACTTGGGGGAAC GCTGAAGACCTAG >SEQ ID NO: 36 TEVTRKKNRVKLSGTLGYVAPEYVLDGKLTDKSDVYAFGVVLLELLLRRRPLEIVAPTQCQSIVTWAM PQLTDRTKLPDIVDPVIRDAMDVKHLYQAAAVAVLCLQPEPIYRPLITDVLHSLIPLVPVELGGTLKT >SEQ ID NO: 37 ACGAGGCCTCGTGCCATACTTTTGGATTCAGATTTCAATGCCAAGATTTCGGATTTCGGTCTTGCAG TGTCAAGTGGAAATCGCACCAAAGGTAATCTGAAGCTTTCCGGAACTTTGGGCTATGTTGCTCCTG AGTACTTATTAGACGGGAAGTTGACAGAGAAGAGTGATGTATATGCGTTCGGAGTAGTACTTCTTG AGCTTTTGTTAGGAAGGAGGCCAATTGAGAAGATGGCCCCATCTCAATGCCAATCAATTGTTACAT GGGCCATGCCTCAGCTAATTGACAGATCAAAGCTCCCAACCATAATTGACCCCGTGATCAGGAACA CGATGGACCTGAAGCACTTGTACCAAGTTGCTGCAGTGGCTGTGCTCTGTGTGCAGCCAGAACCAA GTTATAGGCCACTAATCACAGATGTGCTCCACTCTCTGATTCCCCTGGTGCCCATGGAGCTCGGAG GGTCACTGAGGGCTACCTTGGAATCGCCTCGCGTATCACAACATCGTTCTCCCTGCTGA >SEQ ID NO: 38 TRPRAILLDSDFNAKISDFGLAVSSGNRTKGNLKLSGTLGYVAPEYLLDGKLTEKSDVYAFGVVLLELL LGRRPIEKMAPSQCQSIVTWAMPQLIDRSKLPTIIDPVIRNTMDLKHLYQVAAVAVLCVQPEPSYRPLIT DVLHSLIPLVPMELGGSLRATLESPRVSQHRSPC >SEQ ID NO: 39 CCTTTATTGAATAGATTGAACTCCTTCCGTGGTTCTAGGAGAAAGGGATGTGCATATATAATTGAAT ATTCTCTGCTGCAAGCAGCCACAAATAATTTTAGTACAAGTGACATCCTTGGAGAGGGTGGTTTTG GGTGTGTATACAGAGCTAGGTTAGATGATGATTTCTTTGCTGCTGTGAAGAAGTTAGATGAGGGCA GCAAGCAGGCTGAGTATGAATTTCAGAATGAAGTTGAACTAATGAGCAAAATCAGACATCCAAAT CTTGTTTCTTTGCTGGGGTTCTGCATTCATGGGAAGACTCGGTTGCTAGTCTACGAGCTCATGCAAA ATGGTTCTTTGGAAGACCAATTACATGGGCCATCTCATGGATCCGCACTTACATGGTACCTGCGCAT GAAAATAGCCCTTGATTCAGCAAGGGGTCTAGAACACTTGCACGAGCACTGCAATCCTGCTGTGAT TCATCGTGATTTCAAATCATCAAATATCCTTCTGGATGCAAGCTTCAATGCCAAGCTTTCAGATTTT GGTCTTGCAGTAACAGCTGCAGGAGGTATTGGTAATGCTAATGTCGAGCTACTGGGCACTTTGGGA TATGTAGCTCCAGAATACCTGCTTGATGGCAAGTTGACGGAGAAAAGTGATGTCTATGGATTTGGA GTTGTTCTTTTGGAGCTAATTATGGGAAGAAAGCCAGTTGATAAATCTGTGGCAACTGAAAGTCAA TCGCTAGTTTC >SEQ ID NO: 40 PLLNRLNSFRGSRRKGCAYIIEYSLLQAATNNFSTSDILGEGGFGCVYRARLDDDFFAAVKKLDEGSKQ AEYEFQNEVELMSKIRHPNLVSLLGFCIHGKTRLLVYELMQNGSLEDQLHGPSHGSALTWYLRMKIAL DSARGLEHLHEHCNPAVIHRDFKSSNILLDASFNAKLSDFGLAVTAAGGIGNANVELLGTLGYVAPEYL LDGKLTEKSDVYGFGVVLLELIMGRKPVDKSVATESQSLVS >SEQ ID NO: 41 ATGAAAATGAAGCTTCTCCTCATGCTTCTTCTTCTTGTTCTTCTTCTTCACCAACCCATTTGGGCTGC AGACCCTCCTGCTTCTTCTCCTGCTTTATCTCCAGGGGAGGAGCAGCATCACCGGAATAATAAAGT GGTAATAGCTATCGTCGTAGCCACCACTGCACTTGCTGCACTCATTTTCAGTTTCTTATGCTTCTGG GTTTATCATCATACCAAGTATCCAACAAAATCCAAATTCAAATCCAAAAATTTTCGAAGTCCAGAT GCAGAGAAGGGGATCACCTTAGCACCGTTTGTGAGTAAATTCAGTTCCATCAAGATTGTTGGCATG GACGGGTATGTTCCAATAATTGACTATAAGCAAATAGAAAAAACGACCAATAATTTTCAAGAAAG TAACATCTTGGGTGAGGGCGGTTTTGGACGTGTTTACAAGGCTTGTTTGGATCATAACTTGGATGTT GCAGTCAAAAAACTACATTGTGAGACTCAACATGCTGAGAGAGAATTTGAGAACGAGGTGAATAT GTTAAGCAAAATTCAGCATCCGAATATAATATCTTTACTGGGTTGTAGCATGGATGGTTACACGAG GCTCGTTGTCTATGAGCTGATGCATAATGGATCATTGGAAGCTCAGTTACATGGACCTTCTCATGGC TCGGCATTGACTTGGCACATGAGGATGAAGATTGCTCTTGACACAGCAAGAGGATTAGAATATCTG CACGAGCACTGTCACCCTGCAGTGATCCATAGGGATATGAAATCTTCTAATATTCTCTTAGATGCA AACTTCAATGCCAAGCTGTCTGATTTTGGTCTTGCCTTAACTGATGGGTCCCAAAGCAAGAAGAAC ATTAAACTATCGGGTACCTTGGGATACGTAGCACCGGAGTATCTTCTAGATGGTAAATTAAGTGAT AAAAGTGATGTCTATGCTTTTGGGGTTGTGCTATTGGAGCTCCTACTAGGAAGGAAGCCAGTAGAA AAACTGGTACCAGCTCAATGCCAATCTATTGTCACATGGGCCATGCCACACCTCACGGACAGATCC AAGCTTCCAAGCATTGTGGATCCAGTGATTAAGAATACAATGGATCCCAAGCACTTGTACCAGGTT GCTGCTGTAGCTGTGCTGTGCGTGCAACCAGAACCTAGTTACCGTCCACTGATCATTGATGTTCTTC ACTCACTCATCCCTCTTGTTCCCATTGAGCTTGGAGGAACACTAAGAGTTTCACAAGTAATT >SEQ ID NO: 42 MKMKLLLMLLLLVLLLHQPIWAADPPASSPALSPGEEQHHRNNKVVIAIVVATTALAALIFSFLCFWVY HHTKYPTKSKFKSKNFRSPDAEKGITLAPFVSKFSSIKIVGMDGYVPIIDYKQIEKTTNNFQESNILGEGGF GRVYKACLDHNLDVAVKKLHCETQHAEREFENEVNMLSKIQHPNIISLLGCSMDGYTRLVVYELMHN GSLEAQLHGPSHGSALTWHMRMKIALDTARGLEYLHEHCHPAVIHRDMKSSNILLDANFNAKLSDFGL ALTDGSQSKKNIKLSGTLGYVAPEYLLDGKLSDKSDVYAFGVVLLELLLGRKPVEKLVPAQCQSIVTW AMPHLTDRSKLPSIVDPVIKNTMDPKHLYQVAAVAVLCVQPEPSYRPLIIDVLHSLIPLVPIELGGTLRVS QVI >SEQ ID NO: 43 ACTCAAGCATCAAAATATTGTAAATCTTTTGGGTATTGTGTTCATGATGACACAAGGTTTTTGGTCT ATGAAATGATGCATCAAGGCTCTTTGGACTCACAATTGCATGGACCAACTCATGGAACCGCATTAA CCTGGCATCGAAGAATGAAAGTCGCACTTGATATTGCTCGAGGATTAGAGTATCTTCATGAACGAT GCAACCCGCCTGTGATTCATAGAGATCTTAAGTCATCGAACATTTTGCTAGATTCCAATTTCAATGC TAAAATTTCGAATTTTGCACTTGCTACCACTGAGCTCCATGCGAAGAACAAAGTTAAGCTTTCGGCT ACTTCTGGTTATTTGGCTCCGGAATACCTATCAGAAGGTAAACTTACCGATAAAAGCGACGTATAT GCATTCGGAGTAGTACTTCTTGGGCTTTTAATCGGTAGAAAACCAGTGGAGAAAATGTCACCATCT

TTATTTCAATCTATTGTCACATGGGCAATGCCTCAGTTAACAGACCGGTCAAAGCTTCCAAACATCG TTGACCCTGTGATTAGAGATACAATGGACCTGAAGCACTTATATCAAGTTGCTGCTGTAGCCGTAC TTTGCGTGCAACCCGAACCAAGTTACAGACCGTTGATTACAGACGTACTACACTCATTCATTCCACT CGTACCCGTTGATCTTGGAGGGTCATTAAGAGCTTAA >SEQ ID NO: 44 TQASKYCKSFGYCVHDDTRFLVYEMMHQGSLDSQLHGPTHGTALTWHRRMKVALDIARGLEYLHER CNPPVIHRDLKSSNILLDSNFNAKISNFALATTELHAKNKVKLSATSGYLAPEYLSEGKLTDKSDVYAFG VVLLGLLIGRKPVEKMSPSLFQSIVTWAMPQLTDRSKLPNIVDPVIRDTMDLKHLYQVAAVAVLCVQPE PSYRPLITDVLHSFIPLVPVDLGGSLRA >SEQ ID NO: 45 CGATCATTTCGTTGCGGCTGTAAAAAACTCCATGGTCCAGAACCAGATGCCCAAAAAGGGTTTGAG AATGAAGTAGATTGGTTAGGTAAACTCAAGCATCAAAATATTGTAAATTTTTTGGGTTATTGTGTTC ATGATGACACAAGGTTTTTGGTCTATGAAATGATGCATCAAGGCTCTTTGGACTCACAATTGCATG GACCAACTCATGGAACCGCATTAACCTGGCATCGAAGAATGAAAGTCGCACTTGATATTGCTCGAG GATTAGAGTATCTTCATGAACGATGCAACCCGCCTGTGATTCATAGAGATCTCAAGTCATCGAACA TTTTGCTAGATTCCAATTTCAATGCTAAAATTTCGAATTTTGCACTTGCTACCACTGAGCTCCATGC GAAGAACAAAGTTAAGCTTTCGGGTACTTCTGGTTATTTGGCTCCGGAATACCTATCCGAAGGTAA ACTTACCGATAAAAGTGATGTATATGCATTCGGAGTAGTACTTCTTGAGCTTTTAATCGGTAGAAA ACCAGTGGAGAAAATGTCACCATCTTTATTTCAATCTATTGTCACATGGGCAATGCCTCAGCTAAC AGACCGGTCAAAGCTTCCAAACATTGTTGACCCTGTGATTAGAGATACAATGGACCTGAAGCACTT GTATCAAGTTGCTGCTGTAGCCGTACTTTGCGTGCAACCCGAACCAAGTTACAGACCGTTGATTAC AGACGTACTACACTCATTCATTCC >SEQ ID NO: 46 RSFRCGCKKLHGPEPDAQKGFENEVDWLGKLKHQNIVNFLGYCVHDDTRFLVYEMMHQGSLDSQLH GPTHGTALTWHRRMKVALDIARGLEYLHERCNPPVIHRDLKSSNILLDSNFNAKISNFALATIELHAKN KVKLSGTSGYLAPEYLSEGKLTDKSDVYAFGVVLLELLIGRKPVEKMSPSLFQSIVTWAMPQLTDRSKL PNIVDPVIRDTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSFIP >SEQ ID NO: 47 ATGATGCATCAAGACTCTTTGGACTCACAATTGCATGGACCAACTCATGGAACCGCATTAACCTGG CATCGAAGAATGAAAGTCGCACTTGATATTGCTCGAGGATTAGAGTATCTTCATGAACGATGCAAC CCGCCTGTGATTCATAGAGATCTCAAGTCATCGAACATTTTGCTAGATTCCAATTTCAATGCTAAAA TTTCGAATTTTGCACTTGCTACCACTGAGCTCCATGCGAAGAACAAAGTTAAGCTTTCGGGTACTTC TGGTTATTTGGCTCCGGAATACCTATCCGAAGGTAAACTTACCGATAAAAGTGATGTATATGCATT CGGAGTAGTACTTCTTGAGCTTTTAATCGGTAGAAAACCAGTGGAGAAAATGTCACCATCTTTATTT CAATCTATTGTCACATGGGCAATGCCTCAGCTAACAGACCGGTCAAAGCTTCCAAACATTGTTGAC CCTGTGATTAGAGATACAATGGACCTGAAGCACTTGTATCAAGTTGCTGCTGTAGCCGTACTTTGC GTGCAACCCGAACCAAGTTACAGACCGTTGATTACAGACGTACTACACTCATTCATTCCACTCGTA CCCGTTGATCTTGGAGGGTCATTAAGAGCTTAA >SEQ ID NO: 48 MMHQDSLDSQLHGPTHGTALTWHRRMKVALDIARGLEYLHERCNPPVIHRDLKSSNILLDSNFNAKIS NFALATTELHAKNKVKLSGTSGYLAPEYLSEGKLTDKSDVYAFGVVLLELLIGRKPVEKMSPSLFQSIV TWAMPQLTDRSKLPNIVDPVIRDTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSFIPLVPVDLGGS LRA >SEQ ID NO: 49 AATTTGAGAGGTGAGCTGGATTTGCTTCAGAGGATTCAGCATTCGAATATAGTGTCCCTTGTGGGC TTCTGCATTCATGAGGAGAACCGCTTCATTGTTTATGAGCTGATGGTGAATGGATCACTTGAAACA CAGCTTCATGGGCCATCACATGGATCAGCTCTGAGTTGGCACATTCGGATGAAGATTGCTCTTGAT ACAGCAAGGGGATTGGAGTATCTTCACGAGCACTGCAATCCACCAATCATCCATAGGGATCTGAAG TCGTCTAACATACTTTTGAATTCAGACTTTAATGCAAAGATTTCAGATTTTGGCCTTGCAGTGACAA GTGGAAATCGCAGCAAAGGGAATCTGAAGCTTTCCGGTACTTTGGGTTATGTTGCCCCTGAGTACT TACTAGATGGGAAGTTGACTGAGAAGAGCGATGTATATGCATTTGGAGTAGTACTTCTTGAGCTTC TTTTGGGAAGGAGGCCAGTTGAGAAGATGGCACCATCTCAGTGTCAATCAATTGTTACATGGGCCA TGCCCCAGCTAATTGACAGATCCAAGCTCCCTACCATAATCGACCCCGTGATCAGGGACACGATGG ATCGGAAGCACTTGTACCAAGTTGCTGCAGTGGCTGTGCTCTGCGTGCAGCCAGAACCAAGCTACA GGCCACTGATCACAGATGTCCTCCACTCTCTGATTCCCCTGGTGCCCATGGACCTTGGAGGGACGCT GAGGATCAACCCGGAATCGCCTTGCACGACACGAAATCAATCTCCCTGCTGA >SEQ ID NO: 50 NLRGELDLLQRIQHSNIVSLVGFCIHEENRFIVYELMVNGSLETQLHGPSHGSALSWHIRMKIALDTARG LEYLHEHCNPPIIHRDLKSSNILLNSDFNAKISDFGLAVTSGNRSKGNLKLSGTLGYVAPEYLLDGKLTE KSDVYAFGVVLLELLLGRRPVEKMAPSQCQSIVTWAMPQLIDRSKLPTIIDPVIRDTMDRKHLYQVAAV AVLCVQPEPSYRPLITDVLHSLIPLVPMDLGGTLRINPESPCTTRNQSPC >SEQ ID NO: 51 CGGGGGCTCTTATCACTCATTGCTGCTGCTACTGCACTGGGTACAAGCTTATTGCTCATGGGTTGCT TCTGGATTTATCATAGAAAGAAAATCCACAAATCTCATGACATTATTCATAGCCCAGATGTAGTTA AAGGTCTTGCATTATCCTCATATATTAGCAAATACAACTCCTTCAAGTCGAATTGTGTGAAACGAC ATGTCTCGTTGTGGGAGTACAATACACTCGAGTCGGCCACAAATAGTTTTCAAGAAAGCGAGATCT TGGGTGGAGGGGGGTTCGGGCTTGTGTACAAGGGAAAACTAGAAGACAACTTGTATGTAGCTGTG AAGAGGCTGGAAGTTGGAAGACAAAACGCAATTAAAGAATTCGAGGCTGAAATAGAGGTATTGGG CACGATTCAGCACCCGAATATAATTTCGTTGTTGGGATATAGCATTCATGCTGACACGAGGCTGCT AGTTTATGAACTGATGCAGAATGGATCTCTGGAGTATCAACTACATGGACCTTCCCATGGATCAGC ATTAGCGTGGCATAATAGATTGAAAATCGCACTTGATACAGCAAGGGGATTAGAATATTTACATGA ACATTGCAAACCACCAGTTATCCATAGAGATCTGAAATCCTCCAATATTCTTCTAGATGCCAACTTC AATGCCAAGATCTCAGATTTTGGTCTTGCTGTGCGCGATGGGGCTCAAAACAAAAATAACATTAAG CTCTCGGGAACCGTTGGCTATGTAGCTCCAGAATACCTATTAGATGGAATACTAACAGATAAAAGT GATGTTTATGGCTTCCGAGTTGTA >SEQ ID NO: 52 RGLLSLIAAATALGTSLLLMGCFWIYHRKKIHKSHDIIHSPDVVKGLALSSYISKYNSFKSNCVKRHVSL WEYNTLESATNSFQESEILGGGGFGLVYKGKLEDNLYVAVKRLEVGRQNAIKEFEAEIEVLGTIQHPNII SLLGYSIHADTRLLVYELMQNGSLEYQLHGPSHGSALAWHNRLKIALDTARGLEYLHEHCKPPVIHRDL KSSNILLDANFNAKISDFGLAVRDGAQNKNNIKLSGTVGYVAPEYLLDGILTDKSDVYGFRVV >SEQ ID NO: 53 GGGGATATACGTGTAGAATCAGCAACAAATAACTTCGGTGAAAGCGAGATATTAGGCGTAGGTGG ATTTGGATGCGTGTATAAAGCTCGACTCGATGATAATTTGCATGTAGCTGTTAAAAGATTAGATGG TATTAGTCAAGACGCCATTAAAGAATTCCAGACGGAGGTGGATCTATTGAGTAAAATTCATCATCC GAATATCATCACCTTATTGGGATATTGTGTTAATGATGAAACCAAGCTTCTTGTTTATGAACTGATG CATAATGGATCTTTAGAAACTCAATTACATGGGCCTTCCAGTGGATCCAATTTAACATGGCATTGC AGGATGAAGATTGCTCTAGATACAGCAAGAGGATTAGAATATTTGCATGAGAACTGCAAACCATC GGTGATTCATAGAGATCTGAAATCATCTAATATCCTTCTGGATTCCAGCTTCAATGCTAAGCTTTCA GATTTTGGTCTTGCTATAATGGATGGGGCCCAGAACAAAAACAACATTAAGCTTTCAGGGACATTG GGTTATGTAGCTCCCGAGTATCTTTTAGATGGAAAATTGACGGATAAAAGTGACGTGTATGCGTTT GGAGTTGTGCTTTTAGAGCTTTTACTTGGAAGGCGACCTGTAGAAAAATTAGCAGAGTCGCAATGC CAATCTATTGTCACTTGGGCTATGCCACAATTAACAGACAGATCAAAGCTTCCGAATATTGTAGAT CCCGTGATCAGATACACAATGGATCTCAAGCACCTGTACCAAGTTGCTGCGGTGGCTGTGTTATGT GTACAACCCGGACCAAGCTACCGGCCATTTATAAACCGACGTCTTGCATTCTCTGATCCCTCTTGTT CCCCGTGA >SEQ ID NO: 54 GDIRVESATNNFGESEILGVGGFGCVYKARLDDNLHVAVKRLDGISQDAIKEFQTEVDLLSKIHHPNIITL LGYCVNDETKLLVYELMHNGSLETQLHGPSSGSNLTWHCRMKIALDTARGLEYLHENCKPSVIHRDLK SSNILLDSSFNAKLSDFGLAIMDGAQNKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLG RRPVEKLAESQCQSIVTWAMPQLTDRSKLPNIVDPVIRYTMDLKHLYQVAAVAVLCVQPGPSYRPFINR RLAFSDPSCSP >SEQ ID NO: 55 AAGTTGAACTGTGAATGTCAATATGCTGAGAGAGAATTTGAGAATGAGGTGGATTTGTTAAGTAAA ATTCAACATCCAAATGTAATTTCTCTACTGGGCTGTAGCAGTAATGAGGATTCAAGGTTTATTGTCT ATGAGTTGATGCAAAATGGATCATTGGAAACTCAATTACATGGACCATCTCATGGCTCAGCATTGA CTTGGCATATGAGGATGAAGATTGCTCTTGACACAGCTAGAGGTTTAAAATATCTGCATGAGCACT GCTACCCTGCAGTGATCCATAGAGATCTGAAATCTTCTAATATTCTTTTAGATGCAAACTTCAATGC CAAGCTTTCTGATTTTGGTCTTGCAATAACTGATGGGTCCCAAAACAAGAATAACATCAAGCTTTC AGGCACATTGGGGTATGTTGCCCCGGAGTATCTTTTAGATGGTAAATTGACAGATAAAAGTGATGT GTATGCTTTTGGAGTTGTGCTTCTTGAGCTTCTATTAGGAAGAAAGCCTGTGGAAAAACTTACACCA TCTCAATGCCAGTCTATTGTCACATGGGCCATGCCACAGCTCACAGACAGATCCAAGCTTCCAAAC ATTGTGGATAATGTGATTAAGAATACAATGGATCCTAAGCACTTATACCAGGTTGCTGCTGTGGCT GTATTATGTGTGCAACCAGAGCCGTGCTACCGCCCTTTGATTGCAGATGTTCTACACTCCCTCATCC CTCTTGTACCTGTTGAGCTTGGAGGAACACTCAGAGTTGCACAAGTGACGCAGCAACCTAAGAATT CTAGTTAA >SEQ ID NO: 56 KLNCECQYAEREFENEVDLLSKIQHPNVISLLGCSSNEDSRFIVYELMQNGSLETQLHGPSHGSALTWH MRMKIALDTARGLKYLHEHCYPAVIHRDLKSSNILLDANFNAKLSDFGLAITDGSQNKNNIKLSGTLGY VAPEYLLDGKLTDKSDVYAFGVVLLELLLGRKPVEKLTPSQCQSIVTWAMPQLTDRSKLPNIVDNVIKN TMDPKHLYQVAAVAVLCVQPEPCYRPLIADVLHSLIPLVPVELGGTLRVAQVTQQPKNSS >SEQ ID NO: 57 CAGTTGCATGGACCTCCTCGTGGATCAGCTTTGAATTGGCATCTTCGCATGGAAATTGCATTGGATG TGGCTAGGGGACTAGAATACCTCCATGAGCGCTGTAACCCCCCTGTAATCCATAGAGATCTCAAAT CGTCTAATGTTCTATTGGATTCCTACTTCAATGCAAAGCTTTCTGACTTTTGGCCTAGCTATAGCTG GATGGAACTTAAACAAGAGCACCGTAAAGTCTTTCGGGAACTCTGGGATATGTGGCTCCAGAGTTA CCTCTTAGATGGGAAATTAACTGATAAGAGTGATGTCTATGCTTTCGGCATTATACTTCTGGAGCTT CTAATGGGGAGAAGACCATTGGAGAAACTAGCAGGAGCTCAGTGCCAATCTATCGTCACATGGGC AATGCCACAGCTTACTGACAGGTCAAAGCTCCCAAATATTGTTGATCCTGTCATCAGAAACGGAAT GGGCCTCAAGCACTTGTATCAAGTTGCTGCTGTAGCCGTGCTATGTGTACAACCAGAACCAAGTTA CCGACCACTGATAACAGATGTCCTGCACTCCTTCATTCCCCTTGTACCAATTGAGCTTGGTGGGTCC TTGAGAGTTGTGGATTCTGCATTATCTGTTAACGCATAA >SEQ ID NO: 58 QLHGPPRGSALNWHLRMEIALDVARGLEYLHERCNPPVIHRDLKSSNVLLDSYFNAKLSDFWPSYSWM ELKQEHRKVFRELWDMWLQSYLLDGKLTDKSDVYAFGIILLELLMGRRPLEKLAGAQCQSIVTWAMP QLTDRSKLPNIVDPVIRNGMGLKHLYQVAAVAVLCVQPEPSYRPLITDVLHSFIPLVPIELGGSLRVVDS ALSVNA >SEQ ID NO: 59 ATGGAGATGGCGCTAACTCCATTGCCGCTCCTGTGTTCGTCCGTCTTGTTCTTGGTGCTATCTTCGTG CTCGTTGGCCAATGGGAGGGATACGCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC TTCTTCTTCTTCTTCTTCTTCTTCTCCGGCGACGTCTACTGTGGCCACCGGCATTTCCGCCGCCGCCG CCGCCGCCGCCAATGGGACGGCCGCCTTGTCTTCGGCAGTTCCGGCGCCTCCGCCTGTTGTGATCGT AGTGCACCACCATTTCCACCGCGAGCTGGTCATCGCCGCCGTCCTCGCCTGCATCGCCACCGTCAC GATCTTCCTTTCCACGCTCTACGCTTGGACACTATGGCGGCGATCTCGCCGGAGCACCGGCGGCAA GGTCACCAGGAGCTCAGACGCAGCGAAGGGGATCAAGCTGGTGCCGATCTTGAGCAGGTTCAACT CGGTGAAGATGAGCAGGAAGAGGCTGGTTGGGATGTTCGAGTACCCGTCGCTGGAGGCAGCGACA GAGAAGTTCAGCGAGAGCAACATGCTCGGTGTCGGCGGGTTTGGCCGCGTCTACAAGGCGGCGTTC GACGCCGGAGTTACCGCGGCGGTGAAGCGGCTCGACGGCGGCGGGCCCGACTGCGAGAAGGAATT CGAGAATGAGCTGGATTTGCTTGGCAGGATCAGGCACCCCAACATTGTGTCCCTCTTGGGCTTCTGT ATCCATGAGGGGAATCACTACATTGTTTATGAGCTGATGGAGAAGGGATCACTGGAAACACAGCTT CATGGGTCTTCACATGGATCAACTCTGAGCTGGCACATCCGGATGAAGATCGCCCTTGACACGGCC AGGGGATTAGAGTACCTTCATGAGCACTGCAGTCCACCAGTGATCCATAGGGATCTGAAATCGTCT AACATACTTTTGGATTCAGACTTCAATGCTAAGATTGCAGATTTTGGTCTTGCTGTGTCTAGTGGGA GTGTCAACAAAGGGAGTGTGAAGCTCTCCGGGACCTTGGGTTATGTAGCTCCTGAGTACTTGTTGG ATGGGAAGTTGACTGAAAAGAGCGATGTATACGCGTTCGGAGTAGTGCTTCTAGAGCTCCTTATGG GGAGGAAGCCTGTTGAGAAGATGTCACCATCTCAGTGCCAATCAATTGTGACATGGGCAATGCCAC AGTTGACCGACAGATCGAAGCTCCCCAGCATAGTTGACCCAGTGATCAAGGACACCATGGATCCA AAACACCTGTACCAAGTTGCAGCAGTGGCTGTTCTATGCGTGCAGGCTGAACCAAGCTACAGGCCA CTGATCACAGATGTGCTCCACTCTCTTGTTCCTCTAGTGCCGACGGAGCTCGGAGGAACACTAAGA GCTGGAGAGCCACCTTCCCCGAACCTGAGGAATTCTCCATGCTGA >SEQ ID NO: 60 MEMALTPLPLLCSSVLFLVLSSCSLANGRDTPSSSSSSSSSSSSSSSSSSSSSSPATSTVATGISAAAAAAAN GTAALSSAVPAPPPVVIVVHHHFHRELVIAAVLACIATVTIFLSTLYAWTLWRRSRRSTGGKVTRSSDAA KGIKLVPILSRFNSVKMSRKRLVGMFEYPSLEAATEKFSESNMLGVGGFGRVYKAAFDAGVTAAVKRL DGGGPDCEKEFENELDLLGRIRHPNIVSLLGFCIHEGNHYIVYELMEKGSLETQLHGSSHGSTLSWHIRM KIALDTARGLEYLHEHCSPPVIHRDLKSSNILLDSDFNAKIADFGLAVSSGSVNKGSVKLSGTLGYVAPE YLLDGKLIEKSDVYAFGVVLLELLMGRKPVEKMSPSQCQSIVTWAMPQLTDRSKLPSIVDPVIKDTMD PKHLYQVAAVAVLCVQAEPSYRPLITDVLHSLVPLVPTELGGTLRAGEPPSPNLRNSPC >SEQ ID NO: 61 TACTCTCTTTTACAAACTGCTACGAACAACTTCAGCTCCTCCAATTTGCTGGGCGAGGGAAGTTTCG GGCATGTGTATAAAGCGAGACTCGATTATGATGTCTATGCCGCTGTAAAGAGACTTACCAGCGTAG GAAAACAGCCCCAAAAAGAACTCCAGGGAGAGGTGGATCTGATGTGCAAGATAAGACATCCCAAC TTGGTGGCTCTCCTGGGCTATTCAAATGACGGCCCAGAGCCCTTGGTTGTGTACGAGCTCATGCAG AATGGTTCACTTCATGATCAGCTTCATGGCCCCTCATGCGGGAGTGCACTCACCTGGTACCTACGAC TAAAGATTGCTCTTGAAGCTGCCAGCAGAGGACTGGAGCACCTGCATGAAAGCTGCAAGCCTGCA ATAATCCACAGAGACTTCAAGGCATCCAACATCCTCTTGGACGCCAGCTTCAATGCGAAGGTGTCC GACTTTGGTATAGCGGTAGCTCTGGAGGAAGGTGGCGTGGTGAAAGACGACGTACAAGTGCAAGG CACCTTCGGGTACATTGCTCCTGAGTACCTGATGGACGGGACATTGACAGAGAAGAGTGATGTTTA CGGATTTGGAGTAGTATTGCTTGAGCTGCTGACAGGCAGACTGCCCATTGATACGTCCTTACCACTC GGATCGCAATCTCTAGTGACATGGGTAACACCCATACTAACTAACCGAGCAAAGCTGATGGAAGTT ATCGACCCCACCCTTCAAGATACGCTGAACGTGAAGCAACTTCACCAGGTGGCCGCAGTGGCAGTC CTTTGCGTCCAAGCGGAACCCAGCTACCGCCCTCTCATCGCCGACGTGGTTCAGTCACTGGCTCCGC TGGTGCCTCAAGAGCTCGGCGGCGCATTGCGA >SEQ ID NO: 62 YSLLQTATNNFSSSNLLGEGSFGHVYKARLDYDVYAAVKRLTSVGKQPQKELQGEVDLMCKIRHPNLV ALLGYSNDGPEPLVVYELMQNGSLHDQLHGPSCGSALTWYLRLKIALEAASRGLEHLHESCKPAIIHRD FKASNILLDASFNAKVSDFGIAVALEEGGVVKDDVQVQGTFGYIAPEYLMDGTLTEKSDVYGFGVVLL ELLTGRLPIDTSLPLGSQSLVTWVTPILTNRAKLMEVIDPTLQDTLNVKQLHQVAAVAVLCVQAEPSYR PLIADVVQSLAPLVPQELGGALR >SEQ ID NO: 63 ACCTCAGATGCCTATAGGGGTATTCCACTCATGCCTCTCCTGAATCGTTTGAACTCCCGTATTTCCA AGAAGAAGGGATGTGCAACTGCAATTGAATATTCTAAGCTGCAAGCAGCTACAAATAACTTCAGC AGCAATAACATTCTTGGAGAGGGTGGATTTGCGTGTGTATACAAGGCCATGTTTGATGATGATTCC TTTGCTGCTGTGAAGAAGCTAGATGAGGGTAGCAGACAGGCTGAGCATGAATTTCAGAATGAAGT GGAGCTGATGAGCAAAATCCGACATCCAAACCTTGTTTCTTTGCTTGGGTTCTGCTCTCATGAAAAT ACACGGTTCTTAGTATATGATCTGATGCAGAATGGCTCTTTGGAAGACCAATTACATGGGCCATCT CACGGATCTGCACTTACATGGTTTTTGCGCATAAAGATAGCACTTGATTCAGCAAGGGGTCTAGAA CACTTGCATGAGCACTGCAACCCTGCAGTGATTCATCGAGATTTCAAATCATCAAATATTCTTCTTG ATGCAAGCTTCAACGCCAAGCTTTCAGATTTTGGTCTTGCAGTAACAAGTGCAGGATGTGCTGGCA ATACAAATATTGATCTAGTAGGGACATTGGGATATGTAGCTCCAGAATACCTACTTGATGGTAAAT TGACAGAGAAAAGTGATGTCTATGCATATGGAGTTGTTTTGTTGGAGCTACTTTTTGGAAGAAAGC CAATTGATAAATCTCTACCAAGTGAATGCCAATCTCTCATTTCTTGGGCAATGCCACAGCTAACAG ATAGAGAAAAGCTCCCAACTATAGTAGACCCCATGATCAAAGGCACAATGAACTTGAAACACCTA TATCAAGTAGCAGCTGTTGCAATGCTATGTGTGCAGCCAGAACCCAGTTACAGGCCATTAATAGCT GACGTTGTGCACTCTCTCATTCCTCTCGTACCAATAGAACTCGGGGGAACTTTAAAGCTCTCTAATG CACGACCCACTGAGATGAAGTTATTTACTTCTTCCCAATGCAGTGTTGAGATTGCTTCCAACCCAAA ATTGTGA >SEQ ID NO: 64 TSDAYRGIPLMPLLNRLNSRISKKKGCATAIEYSKLQAATNNFSSNNILGEGGFACVYKAMFDDDSFAA VKKLDEGSRQAEHEFQNEVELMSKIRHPNLVSLLGFCSHENTRFLVYDLMQNGSLEDQLHGPSHGSAL TWFLRIKIALDSARGLEHLHEHCNPAVIHRDFKSSNILLDASFNAKLSDFGLAVTSAGCAGNTNIDLVGT LGYVAPEYLLDGKLTEKSDVYAYGVVLLELLFGRKPIDKSLPSECQSLISWAMPQLTDREKLPTIVDPMI KGTMNLKHLYQVAAVAMLCVQPEPSYRPLIADVVHSLIPLVPIELGGTLKLSNARPTEMKLFTSSQCSV EIASNPKL >SEQ ID NO: 65 AATTCGGCACGAGGAGAACACTTGCACGAGCACTGCAACCCTGCAGTGATTCACCGAGATTTCAAA TCATCAAATATTCTTCTTGATGCAAGCTTCAACGCCAAGCTTTCAGATTTTGGTCTTGCAGTAAAAA GTGCAGGATGTGCTGGTAACACAAATATTGATCTAGTAGGGACATTGGGATATGTAGCTCCAGAAT ACATGCTTGATGGTAAATTGACAGAGAAAAGTGATGTCTATGCATATGGAGTTGTTTTGTTAGAGC TACTTTTTGGAAGAAAGCCAATTGATAAATCTCTACCAAGTGAATGCCAATCTCTCATTTCTTGGGC AATGCCACAGCTAACAGATAGAGAAAAGCTCCCGACTATAATAGATCCCATGATCAAAGGCGCAA TGAACTTGAAACACCTATATCAAGTGGCAGCTGTTGCAGTGCTATGTGTGCAGCCAGAACCCAGTT ACAGGCCATTAATAGCTGACGTTGTGCACTCTCTCATTCCTCTCGTACCAGTAGAACTTGGGGGAA CATTAAAGTCATCACCCACTGAGATGAAGTCATTTGCTTCTTCCCAATGCAGTGCCCACGTTGCTTC >SEQ ID NO: 66 NSARGEHLHEHCNPAVIHRDFKSSNILLDASFNAKLSDFGLAVKSAGCAGNTNIDLVGTLGYVAPEYML DGKLIEKSDVYAYGVVLLELLFGRKPIDKSLPSECQSLISWAMPQLTDREKLPTIIDPMIKGAMNLKHLY QVAAVAVLCVQPEPSYRPLIADVVHSLIPLVPVELGGTLKSSPTEMKSFASSQCSAHVAS

>SEQ ID NO: 67 ATGTTCTTGTTTCCTAAAACAGTTCCTATTTGGTTTTTTCATCTGTGTCTAGTAGCAGTTCATGCCAT ACAAGAAGACCCACCTGTCCCTTCACCATCTCCCTCTCTCATTTCTCCTATTTCAACTTCAATGGCTG CCTTCTCTCCAGGGGTTGAATCGGAAATGGGAATCAAAGACCACCCCCAGCATGATGACCTCCACA GGAAAATAATCTTGTTGCTCACTGTTGCTTGTTGCATACTTGTTATCATCCTTCTTTCTTTGTGTTCTT GTTTCATTTACTATAAGAAGTCCTCACAAAAGAAAAAAGCTACTCGGTGTTCAGATGTGGAGAAAG GGCTTTCATTGGCACCATTTTTGGGCAAATTCAGTTCCTTGAAAATGGTTAGTAATAGGGGATCTGT TTCATTAATTGAGTATAAGATACTAGAGAAAGGAACAAACAATTTTGGCGATGATAAATTGTTGGG AAAGGGAGGATTTGGACGTGTATATAAGGCTGTAATGGAAGATGACTCAAGTGCTGCAGTCAAGA AACTAGACTGCGCAACTGATGATGCGCAGAGAGAATTTGAGAATGAGGTGGATTTGTTAAGCAAA TTTCACCATCCAAATATAATTTCTATTGTGGGTTTTAGTGTTCATGAGGAGATGGGGTTCATTATTT ATGAGTTAATGCCAAATGGGTGCCTTGAAGATCTACTGCATGGACCTTCTCGTGGATCTTCACTAA ATTGGCATTTAAGGTTGAAAATTGCTCTTGATACAGCAAGAGGATTAGAATATCTGCATGAATTCT GCAAGCCAGCAGTGATCCATAGAGATCTGAAATCATCGAATATTCTTTTGGACGCCAACTTCAATG CCAAGCTGTCAGATTTTGGTCTTGCTGTAGCTGATAGCTCTCATAACAAGAAAAAGCTCAAGCTTTC AGGCACTGTGGGTTATGTAGCCCCAGAGTATATGTTAGATGGTGAATTGACGGATAAGAGTGATGT CTATGCTTTTGGAGTTGTGCTTCTAGAGCTTCTATTAGGAAGAAGGCCTGTAGAAAAACTGACACC AGCTCATTGCCAATCTATAGTAACATGGGCCATGCCTCAGCTCACTAACAGAGCTGTGCTTCCAAC CCTTGTGGATCCTGTGATCAGAGATTCAGTAGATGAGAAGTACTTGTTCCAGGTTGCAGCAGTAGC CGTGTTGTGTATTCAACCAGAGCCAAGTTACCGCCCTCTCATAACAGATGTTGTGCACTCTCTCGTC CCATTAGTTCCTCTTGAGCTTGGAGGGACACTAAGAGTTCCACAGCCTACAACTCCCAGAGGTCAA CGACAAGGCCCATCAAAGAAACTGTTTTTGGATGGTGCTGCCTCTGCT >SEQ ID NO: 68 MFLFPKTVPIWFFHLCLVAVHAIQEDPPVPSPSPSLISPISTSMAAFSPGVESEMGIKDHPQHDDLHRKIIL LLTVACCILVIILLSLCSCFIYYKKSSQKKKATRCSDVEKGLSLAPFLGKFSSLKMVSNRGSVSLIEYKILE KGTNNFGDDKLLGKGGFGRVYKAVMEDDSSAAVKKLDCATDDAQREFENEVDLLSKFHHPNIISIVGF SVHEEMGFIIYELMPNGCLEDLLHGPSRGSSLNWHLRLKIALDTARGLEYLHEFCKPAVIHRDLKSSNIL LDANFNAKLSDFGLAVADSSHNKKKLKLSGTVGYVAPEYMLDGELTDKSDVYAFGVVLLELLLGRRP VEKLTPAHCQSIVTWAMPQLTNRAVLPTLVDPVIRDSVDEKYLFQVAAVAVLCIQPEPSYRPLITDVVH SLVPLVPLELGGTLRVPQPTTPRGQRQGPSKKLFLDGAASA >SEQ ID NO: 69 GCTGCTGCGGTGAAGAGATTGGATGGTGGGGCTGGGGCACATGATTGCGAGAAGGAATTCGAGAA TGAGTTAGATTTGCTTGGAAAGATTCGGCATCCGAACATTGTGTCCCTTGTGGGCTTCTGTATTCAT GAGGAGAACCGTTTCATTGTTTATGAGCTGATAGAGAATGGGTCGTTGGATTCACAACTTCATGGG CCATCACATGGTTCAGCTCTGAGCTGGCATATTCGGATGAAGATTGCTCTTGACACGGCAAGGGGA TTAGAGTACCTGCATGAGCACTGCAACCCACCAGTTATCCATAGGGATCTGAAGTCATCTAACATA CTTTTAGATTCAGACTTCAGTGCTAAGATTTCAGATTTTGGCCTTGCGGTGATTAGTGGGAATCACA GCAAAGGGAATTTAAAGCTTTCTGGGACTATGGGCTATGTGGCCCCTGAGTACTTATTGGATGGGA AGTTGACTGAGAAGAGCGATGTATATGCGTTTGGGGTGGTACTTCTAGAACTTCTACTGGGAAGGA AACCTGTTGAGAAGATGGCACAATCTCAATGCCAATCAATTGTTACATGGGCCATGCCTCAGCTAA CTGATAGATCCAAACTCCCTAACATAATTGATCCCATGATCAAGAACACAATGGATCTGAAACACT TGTACCAAGTTGCTGCAATGGCTGTGCTCTGA >SEQ ID NO: 70 AAAVKRLDGGAGAHDCEKEFENELDLLGKIRHPNIVSLVGFCIHEENRFIVYELIENGSLDSQLHGPSHG SALSWHIRMKIALDTARGLEYLHEHCNPPVIHRDLKSSNILLDSDFSAKISDFGLAVISGNHSKGNLKLSG TMGYVAPEYLLDGKLIEKSDVYAFGVVLLELLLGRKPVEKMAQSQCQSIVTWAMPQLTDRSKLPNIID PMIKNTMDLKHLYQVAAMAVL >SEQ ID NO: 71 ACCCTCGGTTATGTAGCTCCTGAGTATCTGTTAGATGGTAAGTTAACAGAGAAAAGCGATGTGTAT GGGTTTGGAGTAGTGTTACTCGAGCTTCTGCTTGGGAAGAAGCCTATGGAGAAAGTGGCAACAACA GCAACTCAGTGCCAGATGATAGTCACATGGACCATGCCTCAGCTCACTGACAGAACGAAACTTCCG AATATCGTGGATCCGGTGATCAGAAACTCCATGGATTTAAAGCACTTGTACCAGGTTGCTGCTGTG GCAGTATTGTGTGTGCAGCCAGAACCGAGTTATCGGCCATTGATAACTGATATTTTGCATTCTCTTG TGCCCCTTGTCCCTGTTGAGCTTGGTGGGACGCTCAGGAACTCGATAACAATGGCTACAACAACAA TATCTCCTGAAAGCTAA >SEQ ID NO: 72 TLGYVAPEYLLDGKLTEKSDVYGFGVVLLELLLGKKPMEKVATTATQCQMIVTWTMPQLTDRTKLPNI VDPVIRNSMDLKHLYQVAAVAVLCVQPEPSYRPLITDILHSLVPLVPVELGGTLRNSITMATTTISPES >SEQ ID NO: 73 CGGCACGAGGGGCTGGTGGCCATGATCGAGTACCCGTCGCTGGAGGCGGCGACGGGCAAGTTCAG CGAGAGCAACGTGCTCGGCGTCGGCGGGTTCGGCTGCGTCTACAAGGCGGCGTTCGACGGCGGCG CCACCGCCGCCGTGAAGAGGCTCGAAGGCGGCGAGCCGGACTGCGAGAAGGAGTTCGAGAATGAG CTGGACTTGCTTGGCAGGATCAGGCACCCAAACATAGTGTCCCTCCTGGGCTTCTGCGTCCATGGT GGCAATCACTACATTGTTTATGAGCTCATGGAGAAGGGATCATTGGAGACACAACTGCATGGGCCT TCACATGGATCGGCTATGAGCTGGCACGTCCGGATGAAGATCGCGCTCGACACGGCGAGGGGATT AGAGTATCTTCATGAGCACTGCAATCCACCAGTCATCCATAGGGATCTGAAATCGTCTAATATACT CTTGGATTCAGACTTCAATGCTAAGATTGCAGATTTTGGCCTTGCAGTGACAAGTGGGAATCTTGA CAAAGGGAACCTGAAGATCTCTGGGACCTTGGGATATGTAGCTCCCGAGTACTTATTAGATGGGAA GTTGACCGAGAAGAGCGACGTCTACGCGTTTGGAGTAGTGCTTCTAGAGCTCCTGATGGGGAGGAA GCCTGTTGAGAAGATGTCACCATCTCAGTGCCAATCAATTGTGTCATGGGCCATGCCTCAGCTAAC CGACAGATCGAAGCTACCCAACATCATCGACCCGGTGATCAAGGACACAATGGACCCAAAGCATT TATACCAAGTTGCGGCGGTGGCCGTTCTATGCGTGCAGCCCGAACCGAGTTACAGACCGCTGATAA CAGACGTTCTCCACTCCCTTGTTCCTCTGGTACCCGCGGATCTCGGGGGGAACGCTCAGAGTTACA GAGCCGCATTCTCCACACCAAATGTACCATCCCTCTTGAGAAGTGATCCTACAAGTTTCGTCGAAG CGGGGAAAGCGAATNTATACGGTCCAGCGGTAGATGGCTGTTATTTTGGTACTTATATCTCACCCT GTCCTGCTGCTTATCTTAGGATGAGTGANGAGCTCCNACCTGCTGCTTTTGCTGGTTGGGCAGAGA GAATACAGTTCTGGTTAGGATTG >SEQ ID NO: 74 RHEGLVAMIEYPSLEAATGKFSESNVLGVGGFGCVYKAAFDGGATAAVKRLEGGEPDCEKEFENELDL LGRIRHPNIVSLLGFCVHGGNHYIVYELMEKGSLETQLHGPSHGSAMSWHVRMKIALDTARGLEYLHE HCNPPVIHRDLKSSNILLDSDFNAKIADFGLAVTSGNLDKGNLKISGTLGYVAPEYLLDGKLTEKSDVY AFGVVLLELLMGRKPVEKMSPSQCQSIVSWAMPQLTDRSKLPNIIDPVIKDTMDPKHLYQVAAVAVLC VQPEPSYRPLITDVLHSLVPLVPADLGGNAQSYRAAFSTPNVPSLLRSDPTSFVEAGKANXYGPAVDGC YFGTYISPCPAAYLRMSXELXPAAFAGWAERIQFWLGL >SEQ ID NO: 75 ATGAAAGTGATTGGGAGAAAGGGTTATGTCTCTTTTATTGATTATAAGGTACTAGAAACTGCAACA AACAATTTTCAGGAAAGTAATATCCTGGGTGAGGGCGGGTTTGGTTGCGTCTACAAGGCGCGGTTG GATGATAACTCCCATGTGGCTGTGAAGAAGATAGATGGTAGAGGCCAGGATGCTGAGAGAGAATT TGAGAATGAGGTGGATTTGTTGACTAAAATTCAGCACCCAAATATAATTTCTCTCCTGGGTTACAG CAGTCATGAGGAGTCAAAGTTTCTTGTCTATGAGCTGATGCAGAATGGATCTCTGGAAACTGAATT GCACGGACCTTCTCATGGATCATCTCTAACTTGGCATATTCGAATGAAAATCGCTCTGGATGCAGC AAGAGGATTAGAGTATCTACATGAGCACTGCAACCCACCAGTCATCCATAGAGATCTTAAATCATC TAATATTCTTCTGGATTCAAACTTCAATGCCAAGCTTTCGGATTTTGGTCTAGCTGTAATTGATGGG CCTCAAAACAAGAACAACTTGAAGCTTTCAGGCACCCTGGGTTATCTAGCTCCTGAGTATCTTTTAG ATGGTAAACTGACTGATAAGAGTGATGTGTATGCATTTGGAGTGGTGCTTCTAGAGCTACTACTGG GAAGAAAGCCTGTGGAAAAACTGGCACCAGCTCAATGCCAGTCCATTGTCACATGGGCCATGCCA CAGCTGACTGACAGATCAAAGCTCCCAGGCATCGTTGACCCTGTGGTCAGAGACACGATGGATCTA AAGCATTTATACCAAGTTGCTGCTGTAGCTGTGCTATGTGTGCAACCAGAACCAAGTTACCGGCCA TTGATAACAGATGTTCTGCACTCCCTCATCCCACTCGTTCCAGTTGAGTTGGGAGGGATGCTAAAA GTTACCCAGCAAGCGCCGCCTATCAACACCACTGCACCTTCTGCTGGAGGTTGA >SEQ ID NO: 76 MKVIGRKGYVSFIDYKVLETATNNFQESNILGEGGFGCVYKARLDDNSHVAVKKIDGRGQDAEREFEN EVDLLTKIQHPNIISLLGYSSHEESKFLVYELMQNGSLETELHGPSHGSSLTWHIRMKIALDAARGLEYL HEHCNPPVIHRDLKSSNILLDSNFNAKLSDFGLAVIDGPQNKNNLKLSGTLGYLAPEYLLDGKLTDKSD VYAFGVVLLELLLGRKPVEKLAPAQCQSIVTWAMPQLTDRSKLPGIVDPVVRDTMDLKHLYQVAAVA VLCVQPEPSYRPLITDVLHSLIPLVPVELGGMLKVTQQAPPINTTAPSAGG >SEQ ID NO: 77 ATGCCGCCGCCATCGCCGCTCCTCCGTTCCTCCGCCTTCGTCGTCTTGCTGCTCCTGGTGTGTCGCCC GTTGTTGGTCGCCAATGGGAGGGCCACGCCGCCTTCTCCGGGATGGCCACCGGCGGCTCAGCCCGC GCTGCAGCCTGCACCCACCGCCAGCGGCGGCGTGGCCTCCGTGCTTCCTTCGGCCGTGGCGCCTCC TCCCTTAGGTGTGGTTGTGGCGGAGAGGCACCACCACCTCAGCAGGGAGCTCGTCGCTGCCATTAT CCTCTCATCCGTCGCCAGCGTCGTGATCCCCATTGCCGCGCTGTATGCCTTCTTGCTGTGGCGACGA TCACGGCGAGCCCTGGTGGATTCCAAGGACACCCAGAGCATAGATACCGCAAGGATTGCTTTTGCG CCGATGTTGAACAGCTTTGGCTCGTACAAGACTACCAAGAAGAGTGCCGCGGCGATGATGGATTAC ACATCTTTGGAGGCAGCGACAGAAAACTTCAGTGAGAGCAATGTCCTTGGATTTGGTGGGTTTGGG TCTGTGTACAAAGCCAATTTTGATGGGAGGTTTGCTGCTGCGGTGAAGAGACTGGATGGTGGGGCA CATGATTGCAAGAAGGAATTCGAGAATGAGCTAGACTTGCTTGGGAAGATTCGACATCCGAACATC GTGTCCCTTGTGGGCTTCTGCATTCATGAGGAGAACCGTTTCGTTGTTTATGAGCTGATGGAGAGTG GGTCGTTGGATTCGCAACTTCATGGGCCATCACATGGTTCAGCTCTGAGCTGGCATATTCGGATGA AGATTGCTCTCGACACAGCAAGGGGATTAGAGTACCTGCATGAGCACTGCAACCCACCGGTTATCC ATAGGGATCTTAAGTCATCTAACATACTTTTAGATTCAGACTTCAGCGCTAAGATTTCAGACTTTGG CCTGGCAGTGACTAGTGGGAATCACAGCAAAGGGAATTTAAAGCTTTCTGGGACTATGGGCTATGT GGCTCCTGAGTACTTATTAGATGGGAAGCTGACTGAGAAGAGCGATGTATACGCGTTTGGGGTAGT ACTTCTAGAACTCCTGCTGGGAAGGAAACCTGTCGAGAAGATGGCACAATCTCAGTGCCGATCAAT CGTTACATGGGCCATGCCTCAGCTAACTGATAGATCCAAGCTCCCGAACATAATTGATCCCATGAT CAAGAACACAATGGATCTGAAACACTTGTACCAAGTTGCTGCAGTGGCCGTGCTCTGCGTGCAGCC AGAGCCGAGTTACAGGCCACTGATCACCGACGTGCTTCACTCACTGGTACCTCTAGTGCCCACGGA GCTTGGAGGAACGCTGAGGATCGGCCCGGAATCGCCCTACCTACGCTACTAA >SEQ ID NO: 78 MPPPSPLLRSSAFVVLLLLVCRPLLVANGRATPPSPGWPPAAQPALQPAPTASGGVASVLPSAVAPPPLG VVVAERHHHLSRELVAAIILSSVASVVIPIAALYAFLLWRRSRRALVDSKDTQSIDTARIAFAPMLNSFGS YKTTKKSAAAMMDYTSLEAATENFSESNVLGFGGFGSVYKANFDGRFAAAVKRLDGGAHDCKKEFE NELDLLGKIRHPNIVSLVGFCIHEENRFVVYELMESGSLDSQLHGPSHGSALSWHIRMKIALDTARGLEY LHEHCNPPVIHRDLKSSNILLDSDFSAKISDFGLAVTSGNHSKGNLKLSGTMGYVAPEYLLDGKLTEKSD VYAFGVVLLELLLGRKPVEKMAQSQCRSIVTWAMPQLTDRSKLPNIIDPMIKNTMDLKHLYQVAAVAV LCVQPEPSYRPLITDVLHSLVPLVPTELGGTLRIGPESPYLRY >SEQ ID NO: 79 ATGTTGCTCGCGTGTCCTGCAGTGATCATCGTGGAGCGCCACCGTCATTTCCACCGTGAGCTAGTCA TCGCCTCCATCCTCGCCTCAATCGCCATGGTCGCGATTATCCTCTCCACGCTGTACGCGTGGATCCC GCGCAGGCGGTCCCGCCGGCTGCCCCGCGGCATGAGCGCAGACACCGCGAGGGGGATCATGCTGG CGCCGATCCTGAGCAAGTTCAACTCGCTCAAGACGAGCAGGAAGGGGCTCGTGGCGATGATCGAG TACCCGTCGCTGGAGGCAGCGACAGGGGGGTTCAGTGAGAGCAACGTGCTCGGCGTAGGCGGCTT CGGTTGCGTCTACAAGGCAGTCTTCGATGGCGGCGTTACCGCGGCGGTCAAGAGGCTGGAGGGAG GTGGCCCTGAGTGCGAGAAGGAATTCGAGAATGAGCTGGATCTGCTTGGCAGGATTCGGCACCCC AACATCGTGTCCCTGCTGGGCTTTTGTGTTCACGAGGGGAATCACTACATTGTTTATGAGCTCATGG AGAAGGGATCCCTGGACACACAGCTGCATGGGGCCTCACATGGATCAGCGCTGACCTGGCATATCC GGATGAAGATCGCACTCGACATGGCCAGGGGATTAGAATACCTCCATGAGCACTGCAGTCCACCA GTGATCCATAGGGATCTGAAGTCATCTAACATACTTTTAGATTCTGACTTCAATGCTAAGATTTCAG ATTTTGGTCTTGCAGTGACCAGTGGGAACATTGACAAGGGAAGCATGAAGCTTTCTGGGACCTTGG GTTATGTGGCCCCTGAGTACCTATTAGATGGGAAGCTGACTGAAAAGAGTGACGTATATGCATTTG GAGTGGTGCTTCTTGAGCTACTAATGGGAAGGAAGCCTGTCGAGAAGATGAGTCAAACTCAGTGCC AATCAATTGTGACGTGGGCCATGCCGCAGCTGACTGACAGAACAAAACTTCCCAACATAGTTGACC CAGTGATCAGGGACACCATGGATCCAAAGCATTTGTACCAAGTGGCAGCAGTGGCAGTTCTATGTG TGCAACCAGAACCAAGTTACAGACCGCTGATTACTGATGTTCTCCACTCTCTTGTCCCTCTAGTCCC TGTGGAGCTCGGAGGGACACTGAGGGTTGTAGAGCCACCTTCCCCAAACCTAAAACATTCTCCTTG T >SEQ ID NO: 80 MLLACPAVIIVERHRHFHRELVIASILASIAMVAIILSTLYAWIPRRRSRRLPRGMSADTARGIMLAPILSK FNSLKTSRKGLVAMIEYPSLEAATGGFSESNVLGVGGFGCVYKAVFDGGVTAAVKRLEGGGPECEKEF ENELDLLGRIRHPNIVSLLGFCVHEGNHYIVYELMEKGSLDTQLHGASHGSALTWHIRMKIALDMARGL EYLHEHCSPPVIHRDLKSSNILLDSDFNAKISDFGLAVTSGNIDKGSMKLSGTLGYVAPEYLLDGKLTEK SDVYAFGVVLLELLMGRKPVEKMSQTQCQSIVTWAMPQLTDRTKLPNIVDPVIRDTMDPKHLYQVAA VAVLCVQPEPSYRPLITDVLHSLVPLVPVELGGTLRVVEPPSPNLKHSPC >SEQ ID NO: 81 ATGAAGAAGAAGCTTGTGCTGCATCTGCTTCTTTTCCTTGTTTGTGCTCTTGAAAACATTGTTTTGGC CGTACAAGGCCCTGCTTCATCACCCATTTCTACTCCCATCTCTGCTTCAATGGCTGCCTTCTCTCCAG CTGGGATTCAACTTGGAGGTGAGGAGCACAAGAAAATGGATCCAACCAAGAAAATGTTATTAGCT CTCATTCTTGCTTGCTCTTCATTGGGTGCAATTATCTCTTCCTTGTTCTGTTTATGGATTTATTACAG GAAGAATTCAAGCAAATCCTCTAAAAATGGCGCTAAGAGCTCAGATGGTGAAAAAGGGAATGGTT TGGCACCATATTTGGGTAAATTCAAGTCTATGAGGACGGTTTCCAAAGAGGGTTATGCTTCGTTTAT GGACTATAAGATACTTGAAAAAGCTACAAACAAGTTCCATCATGGTAACATTCTGGGTGAGGGTGG ATTTGGATGTGTTTACAAGGCTCAATTCAATGATGGTTCTTATGCTGCTGTTAAGAAGTTGGACTGT GCAAGCCAAGATGCTGAAAAAGAATATGAGAATGAGGTGGGTTTGCTATGTAGATTTAAGCATTCC AATATAATTTCACTGTTGGGTTATAGCAGTGATAACGATACAAGGTTTATTGTTTATGAGTTGATGG AAAATGGTTCTTTGGAAACTCAATTACATGGACCTTCTCATGGTTCATCATTAACTTGGCATAGGAG GATGAAAATTGCTTTGGATACAGCAAGAGGATTAGAATATCTACATGAGCATTGCAATCCACCAGT CATCCATAGAGATCTGAAATCATCTAATATACTTTTGGATTTGGACTTCAATGCAAAGCTTTCAGAT TTTGGTCTTGCAGTAACTGATGCGGCAACAAACAAGAATAACTTGAAGCTTTCGGGTACTTTAGGT TATCTAGCTCCAGAATACCTTTTAGATGGTAAATTAACAGATAAGAGTGATGTTTATGCATTCGGTG TTGTGCTGCTCGAACTTCTATTGGGACGAAAGGCTGTTGAAAAATTATCACAACTCAGTGCCAATC TTAGGTCCATTTGGGCATAG >SEQ ID NO: 82 MKKKLVLHLLLFLVCALENIVLAVQGPASSPISTPISASMAAFSPAGIQLGGEEHKKMDPTKKMLLALIL ACSSLGAIISSLFCLWIYYRKNSSKSSKNGAKSSDGEKGNGLAPYLGKFKSMRTVSKEGYASFMDYKIL EKATNKFHHGNILGEGGFGCVYKAQFNDGSYAAVKKLDCASQDAEKEYENEVGLLCRFKHSNIISLLG YSSDNDTRFIVYELMENGSLETQLHGPSHGSSLTWHRRMKIALDTARGLEYLHEHCNPPVIHRDLKSSNI LLDLDFNAKLSDFGLAVTDAATNKNNLKLSGTLGYLAPEYLLDGKLTDKSDVYAFGVVLLELLLGRKA VEKLSQLSANLRSIWA >SEQ ID NO: 83 GGAGTGGGAATTGAGAAGCAGCCACCCACCCACCCACCCTATGGATAAAAATAGAAGGCTGTTGA TAGCACTCATTGTAGCTTCTACTGCATTAGGACTAATCTTTATCTTCATCATTTTATTCTGGATTTTT CACAAAAGATTTCACACCTCAGATGTTGTGAAGGGAATGAGTAGGAAAACATTGGTTTCTTTAATG GACTACAACATACTTGAATCAGCCACCAACAAATTTAAAGAAACTGAGATTTTAGGTGAGGGGGG TTTTGGATGTGTGTACAAAGCTAAATTGGAAGACAATTTTTATGTAGCTGTCAAGAAACTAACCCA AAATTCCATTAAAGAATTTGAGACTGAGTTAGAGTTGTTGAGTCAAATGCAACATCCCAATATTAT TTCATTGTTGGGATATTGCATCCACAGTGAAACAAGATTGCTTGTCTATGAACTCATGCAAAATGG ATCACTAGAAACTCAATTACATGGGCCTTCCCGTGGATCAGCATTAACTTGGCATCGCAGGATAAA AATTGCCCTTGATGCAGCAAGAGGAATAGAATATTTACATGAGCAGCGCCATCCCCCTGTAATTCA TAGAGATCTGAAATCATCTAATATTCTTTTAGATTCCAACTTCAATGCAAAGGTAAAACTTTTTATG TAGAAATTATACTAGGACTAGTTTTCCCTCTATTAATCTTGTGTTGTGATTAATTTTAGCTGTCAGAT TTTGGTCTTGCTGTGTTGAGTGGGGCTCAAAACAAAAACAATATCAAGCTTTCTGGAACTATAGGT TATGTAGCGCCTGAATACATGTTAGATGGAAAATTAAGTGATAAAAGTGATGTTTATGGTTTTGGA GTAGTACTTTTGGAGCTGTTATTGGGAAGGCGGCCTGTAGAAAAGGAGGCAGCCACTGAATGTCAG TCTATAGTGACATGGGCCATGCCTCAGCTGACAGATAGATCAAAGCTTCCAAACATTGTTGATCCT GTCATACAAAACACAATGGATTTAAAGCATNTGTATCAGGTTGCTGCAGGTGCTCTATTATGTGTTC AGCCAGAGCCAAGCTATCGTCCCGTATAA >SEQ ID NO: 84 GAGTATCAGTTATTGGAAGCTGCAACTGACAATTTTAGTGAGAGTAATATTTTGGGAGAAGGTGGA TTTGGATGTGTTTACAAAGCATGTTTTGATAACAACTTTCTCGCTGCTGTCAAGAGAATGGATGTTG GTGGGCAAGATGCAGAAAGAGAATTTGAGAAAGAAGTAGATTTGTTGAATAGAATTCAGCATCCG GATATAATTTCCCTGTTGGGTTATTGTATTCATGATGAGACAAGGTTCATCATTTATGAACTAATGC AGAACGGATCTTTGGAAAGACAATTACATGGACCTTCTCATGGATCGGCTTTAACTTGGCATATCC GGATGAAAATTGCACTTGATACAGCAAGAGCATTAGAATATCTCCATGAGAATTGCAACCCTCCTG TGATCCACAGAGATCTGAAATCATCCAATATACTTTTGGATTCTAATTTCAAGGCCAAGATTTCAGA TTTTGGTCTTGCTGTAATTTCTGGGAGTCAAAACAAGAACAACATTAAGCTTTCAGGCACTCTTGGT TATGTTGCTCCAGAATATCTGTTAGATGGTAAATTGACTGACAAAAGTGATGTCTATGCTTTTGGGG TTATCCTTCTAGAACTCCTAATGGGAAGAAAACCTGTAGAGAAAATGACACGAACTCAGTGTCAAT CTATCGTTACATGGGCCATGCCTCAACTCACTGATAGATCAAAGCTACCAAACATTGTTGATCCTGT GATTAAAAACACAATGGATTTGAAGCATTTGTTCCAAGTTGCTGCTGTAGCTGTACTGTGTGTACA ACCAGAACCAAGTTACCGGCCATTAATCACAGATGTCCTTCACTCCCTCGTACCCCTTGTTCCTGTC GATCTTGGAGG >SEQ ID NO: 85 EYQLLEAATDNFSESNILGEGGFGCVYKACFDNNFLAAVKRMDVGGQDAEREFEKEVDLLNRIQHPDII SLLGYCIHDETRFIIYELMQNGSLERQLHGPSHGSALTWHIRMKIALDTARALEYLHENCNPPVIHRDLK SSNILLDSNFKAKISDFGLAVISGSQNKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVILLELLMGR KPVEKMTRTQCQSIVTWAMPQLTDRSKLPNIVDPVIKNTMDLKHLFQVAAVAVLCVQPEPSYRPLITDV LHSLVPLVPVDLGG >SEQ ID NO: 86 TGTGCTCATGATGAGACCAAACTACTTGTTTACGAACTTATGCACAATGGTTCGTTAGAAACTCAAT TACACGGTCCTTCTTGTGGATCCAATTTAACATGGCATTGTCGGATGAAAATTGCGCTAGATATAGC GAGAGGATTGGAATATTTACATGAACACTGCAAACCATCTGTGATTCATAGAGATTTGAAGTCATC TAACATCCTTTTGGATTCAAAATTCAATGCCAAGCTTTCGGATTTCGGTCTTGCTGTGATGAACGGT

GCCAATACCAAAAACATTAAGCTTTCGGGGACGTTGGGTTACGTAGCTCCCGAGTATCTTTTAAAT GGGAAATTGACCGATAAAAGTGACGTCTACGCATTCGGAGTTGTACTTTTAGAGCTTCTACTCAAA AGGCGGCCTGTCGAAAAACTAGCACCATCCGAGTGCCAGTCCATCGTCACTTGGGCTATGCCGCAA CTAACAGACAGAACAAAGCTTCCGAGTGTTATAGATCCCGTGATCAGGGACACGATGGATCTTAAA CACTTGTATCAAGTGGCGGCTGTGGCTGTGTTGTGTGTTCAACCGGAACCGGGATACCGGCCGTTG ATAACCGACGTCTTGCATTCTCTGGTTCCTCTCGTGCCGGTTGAACTCGGAGGGACTCTACGAGTTG CGGAAACAGGTTGCGGCACAGTTGACTTATGA >SEQ ID NO: 87 CAHDETKLLVYELMHNGSLETQLHGPSCGSNLTWHCRMKIALDIARGLEYLHEHCKPSVIHRDLKSSNI LLDSKFNAKLSDFGLAVMNGANTKNIKLSGTLGYVAPEYLLNGKLTDKSDVYAFGVVLLELLLKRRPV EKLAPSECQSIVTWAMPQLTDRTKLPSVIDPVIRDTMDLKHLYQVAAVAVLCVQPEPGYRPLITDVLHS LVPLVPVELGGTLRVAETGCGTVDL >SEQ ID NO: 88 TGGATTTGGATGCGTTTAAAAGCTCAACTCAATGATAACTTATTAGTTGCGGTCAAACGACTAGAC AATAAAAGTCAAAATTCCATCAAAGAATTCCAGACGGAAGTGAATATTTTGAGTAAAATTCAACAT CCAAATATAATTAGTTTGTTGGGATATTGCGATCATGATGAAAGCAAGCTACTTGTTTACGAATTG ATGCAAAATGGTTCTTTAGAAACTCAGTTACATGGGCCTTCTTGTGGATCCAATTTAACATGGTATT GCCGGATGAAAATTGCCCTAGATATAGCAAGAGGATTGGAATATTTACATGAACACTCCAAACCAT CTGTGATTCATAGAGATCTCAAATCATCTAATATACTTCTTGATTCAAATTTCAATGCAAAGCTTTC GGATTTTGGTCTTGCGGTGATGGAAGGTGCAAATAGCAAAAACATTAAACTTTCGGGGACATTGGG ATACGTAGCACCCGAATATCTTTTAGATGGGAAATTAACCGATAAAAGTGACGTGTATGCATTTGG AGTCGTACTTTTTGAGCTTTTACTCAGAAGACGACACGTTGAAAAACTAGAATCATCACAATCCCG CCAATCTATTGTCACTTGGGCGATGCCACTACTAATGGACAGATCGAAGCTTCCGAGTGTGATAGA TCCTGTGATTAGGGATACAATGGATCTTAAACATCTTTATCAAGTGGCTGCGGTGGCGGTGTTGTGT GTTCAATCGGAACCGAGTTACCGTCCGTTGATAACCGATGTTTTACATTCTCTTGTTCCTCTTGTCCC GGTTGAACTTGGAGGGACACTTAGAGTTGTAGAAAAGAGTGTTGT >SEQ ID NO: 89 WIWMRLKAQLNDNLLVAVKRLDNKSQNSIKEFQTEVNILSKIQHPNIISLLGYCDHDESKLLVYELMQN GSLETQLHGPSCGSNLTWYCRMKIALDIARGLEYLHEHSKPSVIHRDLKSSNILLDSNFNAKLSDFGLAV MEGANSKNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLFELLLRRRHVEKLESSQSRQSIVTWAM PLLMDRSKLPSVIDPVIRDTMDLKHLYQVAAVAVLCVQSEPSYRPLITDVLHSLVPLVPVELGGTLRVV EKSVV >SEQ ID NO: 90 ATTCTTTTAGATGCAAACTTCAATGCCAAGCTTTCTGATTTTGGCTTGTCTGTCATTGTTGGAGCAC AAAACAAGAATGATATAAAGCTTTCCGGAACGATGGGTTATGTTGCTCCTGAATATCTTTTAGATG GTAAATTGACTGATAAAAGTGATGTCTATGCTTTTGGAGTTGTGCTTTTGGAGCTTCTTTTAGGAAG AAGGCCTGTTGAAAAACTGGCACCATCTCAATGTCAATCCATTGTCACATGGGCTATGCCTCAACT CACTGATAGATCAAAGTTACCCGATATCGTTGATCCGGTGATCAGACACACAATGGACCCTAAACA TTTATTTCAGGTTGCTGCTGTCGCCGTGCTGTGTGTGCAACCAGAACCGAGCTATCGTCCCCTAATA ACAGATCTTTTGCACTCTCTTATTCCTCTTGTTCCTGTTGAGCTAGGAGGTACTCACAGATCATCAA CATCACAAGCTCCTGTGGCTCCAGCTTAG >SEQ ID NO: 91 ILLDANFNAKLSDFGLSVIVGAQNKNDIKLSGTMGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRP VEKLAPSQCQSIVTWAMPQLTDRSKLPDIVDPVIRHTMDPKHLFQVAAVAVLCVQPEPSYRPLITDLLH SLIPLVPVELGGTHRSSTSQAPVAPA >SEQ ID NO: 92 GATGGGAAGCTCACCGAGAAAAGCGACGTGTACGCGTTTGGCATAGTGCTTCTTGAGCTGCTAATG GGAAGGAAGCCTGTTGAGAAGTTGAGTCAATCTCAGTGCCAATCAATTGTGACTTGGGCCATGCCC CAACTGACAGACAGATCAAAACTTCCCAACATAATTGACCCAGTGATCAGGGACACAATGGATCC AAAGCACTTGTATCAGGTTGCAGCAGTGGCTGTTCTATGCGTGCAACCAGAACCGAGTTACAGACC ACTGATAACGGATGTTCTCCACTCTTTAGTTCCTCTAGTGCCTGTGGAGCTTGGTGGGACACTAAGG GTTGCAGAGCCACCGTCCCCAAACCAAAATCATTCTCCTCGTTGA >SEQ ID NO: 93 DGKLTEKSDVYAFGIVLLELLMGRKPVEKLSQSQCQSIVTWAMPQLTDRSKLPNIIDPVIRDTMDPKHL YQVAAVAVLCVQPEPSYRPLITDVLHSLVPLVPVELGGTLRVAEPPSPNQNHSPR >SEQ ID NO: 94 GGGGTTCATGGCAAGAACAATATAAAACTTTCAGGAACTTTAGGATATGTCGCGCCGGAATACCTT TTAGATGGTAAACTTACTGATAAAAGTGACGTTTATGCGTTTGGAGTTGTGCTTCTCGAGCTTTTGA TAGGACGAAAACCCGTGGAGAAAATGTCACCATTTCAATGCCAATTTATCGTTACATGGGCAATGC CTCAGCTAACGGACAGATCGAAGCTTCCTAATCTTGTGGATCCTGTGATTAGAGATACTATGGACT TGAAGCCCTTATATCAAGTTGCGGCTGTAACTGTGTTATGTGTACAACCCGAACCAAGTTACCGCC CATTAATAACGGATGTTTTGCATTCGTTCATCCCACTTGTACCTGCTGATCTTGGAGGGTCGTTAAA AGTTGTCGACTTTTAA >SEQ ID NO: 95 GVHGKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLIGRKPVEKMSPFQCQFIVTWAMPQ LTDRSKLPNLVDPVIRDTMDLKPLYQVAAVTVLCVQPEPSYRPLITDVLHSFIPLVPADLGGSLKVVDF >SEQ ID NO: 96 ATCGTGTTCCATTTTGGTTGTTGTCTAAAGCTTTCAGATTTTGGTCTTGCTGTAATGGATGGAGCCC AGAACAAAAACAACATCAAGCTTTCAGGGACATTGGGTTATGTAGCTCCAGAGTATCTTTTAGATG GAAAACTGACCGACAAAAGTGATGTATATGCATTTGGAGTTGTACTTTTAGAGCTTCTACTTGGAA GACGGCCTGTAGAAAAACTGGCCGCATCTCAATGCCAATCTATCGTCACTTGGGCCATGCCACAGC TAACAGACAGATCAAAGCTCCCAAATATTGTCGATCCTGTAATCAGATATACGATGGATCTCAAAC ACTTGTACCAAGTTGCTGCCGTGGCAGTGCTGTGTGTGCAACCAGAGCCAAGTTACCGGCCATTAA TAACCGATGTTTTGCATTCTCTTATCCCTCTTGTTCCGGTGGAGCTCGGGGGAACTCTAAAAGCTCC ACAAACAAGGTCTTCGGTAACAAATGACCCGTGA >SEQ ID NO: 97 IVFHFGCCLKLSDFGLAVMDGAQNKNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRR PVEKLAASQCQSIVTWAMPQLTDRSKLPNIVDPVIRYTMDLKHLYQVAAVAVLCVQPEPSYRPLITDVL HSLIPLVPVELGGTLKAPQTRSSVTNDP >SEQ ID NO: 98 CGTGGATCAACTTTAAGTTGGCCTCTCCGAATGAAAATTGCTTTGGATATTGCAAGAGGATTAGAA TACCTTCACGAGCGTTGCAACCCCCCTGTGATCCATAGGCATCTCAAATCGTCTAATATTCTTCTTG ATTCCAGCTTCAACGCAAAGATTTCTGATTTTGGCCTTTCTGTAACTGGCGGAAACCTAAGCAAGA ACATAACCAAGATTTCGGGATCACTGGGTTATCTTGCTCCAGAGTATCTCTTAGACGGTAAACTAA CTGATAAGAGTGATGTGTATGGTTTTGGCATTATTCTTCTAGAGCTTTTGATGGGTAAAAGGCCAGT GGAGAAAGTGGGAGAAACTAAGTGCCAATCAATAGTTACATGGGCTATGCCCCAGCTTACGGACC GATCAAAGCTTCCGAATATTGTTGACCCTACGATCAGGAACACAATGGATGTTAAGCATTTATATC AGGTTGCGGCTGTAGCTGTGTTATGTGTGCAACCGGAGCCAAGCTATAGGCCATTGATAACTGATG TACTACACTCCTTCATTCCACTTGTACCAAATGAACTCGGGGGGTCGCTTAGGGTAGTGGATTCTAC TCCCCATTGCTCATAG >SEQ ID NO: 99 RGSTLSWPLRMKIALDIARGLEYLHERCNPPVIHRHLKSSNILLDSSFNAKISDFGLSVTGGNLSKNITKIS GSLGYLAPEYLLDGKLTDKSDVYGFGIILLELLMGKRPVEKVGETKCQSIVTWAMPQLTDRSKLPNIVD PTIRNTMDVKHLYQVAAVAVLCVQPEPSYRPLITDVLHSFIPLVPNELGGSLRVVDSTPHCS >SEQ ID NO: 100 TTAGATAATGGCGGACCCGATTGTCAACGAGAATTCGAGAATGAGGTTGATTTGATGAGTAGAATT AGGCATCCAAATGTGGTTTCTTTATTGGGTTATTGCATTCATGGAGAAACCAGGCTTCTTGTCTATG AAATGATGCAAAACGGGACGTTGGAATCGCTATTGCATGGACCATCACATGGATCCTCACTAACTT GGCACATTCGTATGAAGATCGCCCTCGACACAGCAAGAGGCCTCGAGTATCTGCATGAACACTGCG ACCCCTCTGTGATCCACCGTGACCTGAAGCCTTCTAACATTCTTTTGGATTCCAACTACAATTCCAA GCTCTCAGACTTTGGTCTTGCAGTCACTGTTGGAAGCCAGAATCAAACCAACATTAAGATTCTAGG GACACTGGGTTACCTTGCACCAGAGTACGTTTTGAATGGCAAATTGACAGAGAAAAGTGATGTGTT TGCTTTTGGAGTTGTCCTGTTGGAGCTTCTCATGGGCAAGAAACCAGTGGAGAAGATGGCATCCCC TCCATGCCAATCCATTGTCACATGGGCGATGCCTCATCTTACTGACAGAATTAAGCTTCCAAATATC ATTGATCCTGTTATTAGAAACACCATGGATCTGAAACACTTGTACCAGGTTGCAGCTGTTGCTGTTC TCTGCGTACAACCAGAGCCCCAGTTATCGTCCTCTGATAACTGA >SEQ ID NO: 101 LDNGGPDCQREFENEVDLMSRIRHPNVVSLLGYCIHGETRLLVYEMMQNGTLESLLHGPSHGSSLTWHI RMKIALDTARGLEYLHEHCDPSVIHRDLKPSNILLDSNYNSKLSDFGLAVTVGSQNQTNIKILGTLGYLA PEYVLNGKLTEKSDVFAFGVVLLELLMGKKPVEKMASPPCQSIVTWAMPHLTDRIKLPNIIDPVIRNTM DLKHLYQVAAVAVLCVQPEPQLSSSDN >SEQ ID NO: 102 TCGGCTCGGCCCAGAACAAGATCGCAAGAC >SEQ ID NO: 103 CTACATTCTCTCCTCGTATTATTCCTCGTTGACT >SEQ ID NO: 104 ACTTTCAGATGAGTGGATCATAACCCTATACA >SEQ ID NO: 105 AGATACAATGGATCTCAAACACTTATACCAG >SEQ ID NO: 106 AAAGGATCCATGGGAAGTGGTGAAGAAGATAGATTTGATGCT >SEQ ID NO: 107 TTTCTGCAGTCTGTGAATCATCTTGTTAACCGGAGAGTCC >SEQ ID NO: 108 TCTGAGTTTTAATCGAGCCAAGTCGTCTCA >SEQ ID NO: 109 TATCCCGGGAAAATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTC >SEQ ID NO: 110 TTTGGATCCTGTGAATCATCTTGTTAACCGGAGAGTCC >SEQ ID NO: 111 ATACCCGGGTCTGTGTCAGGAATCCAAATGGGAAGTGGTGA >SEQ ID NO: 112 AAAGGATCCTCTGTGTCAGGAATCCAAATGGGAAGTGGTGA >SEQ ID NO: 113 AAATCTAGACTGTGAATCATCTTGTTAACCGGAGAGTCC >SEQ ID NO: 114 ATAGAGCTCGCAAGAACCAATCTCCAAAATCCATC >SEQ ID NO: 115 ATAGAGCTCGAGGGTCTTGATATCGAAAAATTGCACG >SEQ ID NO: 116 ATAGGATCCTCGCAAGAACCAATCTCCAAAATCCATC >SEQ ID NO: 117 ATATCTAGACTCGAGGGTCTTGATATCGAAAAATTGCACG >SEQ ID NO: 118 ATATCTAGAAAATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTC >SEQ ID NO: 119 ATAGGATCCTGTTAAAAGCGATTTATAATTTACACCGTTTTGGTGTA >SEQ ID NO: 120 ATACCCGGGAAAAGTTTTTGATGAAATTCAATCTAAAGACT >SEQ ID NO: 121 AAAATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTCTCTAATTCTTTTGATGATCTTCATC ACTGTCTCTGCTTCTTCTGCTTCAAATCCTTCTTTAGCTCCTGTTTACTCTTCCATGGCTACATTCTCT CCTCGAATCCAAATGGGAAGTGGTGAAGAAGATAGATTTGATGCTCATAAGAAACTTCTGATTGGT CTCATAATCAGTTTCTCTTCTCTTGGCCTTATAATCTTGTTCTGTTTTGGCTTTTGGGTTTATCGCAA GAACCAATCTCCAAAATCCATCAACAACTCAGATTCTGAGAGTGGGAATTCATTTTCCTTGTTAATG AGACGACTTGGCTCGATTAAAACTCAGAGAAGAACTTCTATCCAAAAGGGTTACGTGCAATTTTTC GATATCAAGACCCTCGAGAAAGCGACAGGCGGTTTTAAAGAAAGTAGTGTAATCGGACAAGGCGG TTTCGGATGCGTTTACAAGGGTTGTTTGGACAATAACGTTAAAGCAGCGGTCAAGAAGATCGAGAA CGTTAGCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGACTTGTTGAGCAAGATCCATCACTC GAACGTTATATCATTGTTGGGCTCTGCAAGCGAAATCAACTCGAGTTTCATCGTTTATGAGCTTATG GAGAAAGGATCATTAGATGAACAGTTACATGGGCCTTCTCGTGGATCAGCTCTAACATGGCACATG CGTATGAAGATTGCTCTTGATACAGCTAGAGGACTAGAGTATCTCCATGAGCATTGTCGTCCACCA GTTATCCACAGAGATTTGAAATCTTCGAATATTCTTCTTGATTCTTCCTTCAACGCCAAGATTTCAG ATTTCGGTTTTGCTGTATCGCTGGATGAACATGGCAAGAACAACATTAAACTCTCTGGGACACTTG GTTATGTTGCCCCGGAATACCTCCTTGACGGAAAACTGACGGATAAGAGTGATGTTTATGCATTTG GGGTAGTTCTGCTTGAACTCTTGTTGGGTAGACGACCAGTTGAAAAATTAACTCCAGCTCAATGCC AATCTCTTGTAACTTGGGCAATGCCACAACTTACCGATAGATCCAAGCTTCCAAACATTGTGGATG CCGTTATAAAAGATACAATGGATCTCAAACACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCG TGCAGCCAGAACCAAGTTACCGGCCGTTGATAACCGATGTTCTTCACTCACTTGTTCCACTGGTTCC GGTAGAGCTAGGAGGGACTCTCCGGTTAACAAGATGATTCACAG >SEQ ID NO: 122 TCGGACAAGGCGGTTTCGGATGCGT >SEQ ID NO: 123 TAGTCCTCTAGCTGTATCAAGAGCAATCTTCA >SEQ ID NO: 124 TATCATTGTTGGGCTCTGCAAGTGAAATCAAC >SEQ ID NO: 125 TGGAGAAAGGATCCTTAGATGATCAGTTACAT >SEQ ID NO: 126 TCCATGTAACTGATCATCTAAGGATCCTTTC >SEQ ID NO: 127 ATAAACGACGAAACTCGAGTTGATTTCACTTGCAGAG >SEQ ID NO: 128 AAAATGAAGAAACTGGTTCATCTTCAGT >SEQ ID NO: 129 TAGACTTCTATTCTCACATTCTTACAC >SEQ ID NO: 130 TCCAATGATCCATTATGCATCAGCTCA >SEQ ID NO: 131 TCGTTCTCAAATTCTCTCTCAGCATGTTG >SEQ ID NO: 132 TCCGGATATGCCAGGTCAGCGCTGATCCA >SEQ ID NO: 133 TCCAGGGATCCCTTCTCCATGAGCTCAT >SEQ ID NO: 134 AAAGAGCTCTCTGTGTCAGGAATCCAAATGGGAAGTGGTGA >SEQ ID NO: 135 ATAGCTAGCTGTTAAAAGCGATTTATAATTTACACCGTTTTGGTGTA >SEQ ID NO: 136 ATAGCTAGCAGAAAAGTTTTTGATGAAATTCAATCTAAAGACT

>SEQ ID NO: 137 TCTGGGTTTATCATCATACCAAGTATCCA >SEQ ID NO: 138 ATTCAGTTCCATCAAGATTGTTGGCATGGAC >SEQ ID NO: 139 TGGAGGGAGGTGGCCCTGAGTGCGAGAAGGA >SEQ ID NO: 140 GCTGGATCTGCTTGGCAGGATTCGGCA >SEQ ID NO: 141 ATATCTAGATGCTAGGTTATAGATCCATGCA >SEQ ID NO: 142 ATAGGATCCACCAGAACTATATATACGAAGGCA >SEQ ID NO: 143 AGGACGACTTGGCTCGATTAAAATCACAGGTCGTGATATG >SEQ ID NO: 144 TAATCGAGCCAAGTCGTCCTACATATATATTCCTA >SEQ ID NO: 145 TAATCGAGCCAAGTCGTCCTCTCTTTTGTATTCCA >SEQ ID NO: 146 AGGACGACTTGGCTCGATTAAAATCAAAGAGAATCAATGATC >SEQ ID NO: 147 GACGACTTGGCTCGATTAAAA >SEQ ID NO: 148 TGCTAGGTTATAGATCCATGCAAATATGGAGTAGATGTACAAACACACGCTCGGACGCATATTACA CATGTTCATACACTTAATACTCGCTGTTTTGAATTGATGTTTTAGGAATATATATGTAGAGAGAGCT TCCTTGAGTCCATTCACAGGTCGTGATATGATTCAATTAGCTTCCGACTCATTCATCCAAATACCGA GTCGCCAAAATTCAAACTAGACTCGTTAAATGAATGAATGATGCGGTAGACAAATTGGATCATTGA TTCTCTTTGATTGGACTGAAGGGAGCTCCCTCTCTCTTTTGTATTCCAATTTTCTTGATTAATCTTTC CTGCACAAAAACATGCTTGATCCACTAAGTGACATATATGCTGCCTTCGTATATATAGTTCTGGT >SEQ ID NO: 149 TGCTAGGTTATAGATCCATGCAAATATGGAGTAGATGTACAAACACACGCTCGGACGCATATTACA CATGTTCATACACTTAATACTCGCTGTTTTGAATTGATGTTTTAGGAATATATATGTAGGACGACTT GGCTCGATTAAAATCACAGGTCGTGATATGATTCAATTAGCTTCCGACTCATTCATCCAAATACCG AGTCGCCAAAATTCAAACTAGACTCGTTAAATGAATGAATGATGCGGTAGACAAATTGGATCATTG ATTCTCTTTGATTTTAATCGAGCCAAGTCGTCCTCTCTTTTGTATTCCAATTTTCTTGATTAATCTTTC CTGCACAAAAACATGCTTGATCCACTAAGTGACATATATGCTGCCTTCGTATATATAGTTCTGGT >SEQ ID NO: 150 CTTAGCCAATGGATGAGGATGACACGATAATGATAATCAAAGATCAACATGGCACGCTCAAGACC GCCTTTAGAAGTCCTCTCTAAATTCTTTCTTCCGATCTCCTAAATATGTTTTGTTTTGGTCAAATAAA TTGATAGGTAATACTTAGTGATTATACTATTTGGTTTTTGTTTTATCATTGACTATTTCACTTTTATA AATCAAATACTTATCAAAATTGTTCTTTCCGTATGTATTCATATTTTCTAATATTGTAAAGATTTGTT TCACCTAACATCTGTACCCATCTTTGATCATTGACAAAATATATATTAGAATGGCCTTAGAACGTGT TAGGCATCTTCCTACTATTATCATATTACCTAATCCCCAATTTTATTACATTTTTTAATTTCTAAAAG AGCTTGAATATAATGTCATTTCGAATATCTCTGTTCATCTTTTTTTTTTTCTGTGCGACTTCTGACCC AAAGCCTTCGACGATTTTTTCCAATCTGAAAACTTTTGAATAAGGAACTTAGTCAATGGTCAACAC CTTGCTAATTAAACAAAGTTCCATTGATACAATAATGAGATTTTTGTACATTAACGCTTTCATATAG TTTTTGCGATTCAACAGATAATCTTAAAATTAAGGAGTCCTATTGATAAAGTCTTGTTCAAACGTAC AAACTCAATCCACACAAAACCTTCATAAAATACGATATAGGAAATAAAGATTGTTTTTGCGTGAGA AAATACTATATGAACTCAAAAGATTTTAAAACAATTTGTATTAATACATAAACAATTGTTGTGATA CACCCGTGTAAAATTTTAAGATTGTTTTTTTCTGAAATTCTTCAAGGAAACTTATAGCTTAAAATCT ACACTTCAAATACTCTGTTTTAAAGGCATTAAAAATAACTGCGTTTCAGAAAAATATTGAAATTTTA GCTGATCTTTTGCTACAAATTTAAGGAATCTTGGCACCTGCAGAATCTATAACATGTTCATTAAGTA ATGCAATAGTTATACAATTATACATTATTTGCATCATACTTATATTATAGTGATATTAACAAACCCA TGTTCTCAGCACACTTTTACGTAGAAAAACATAAAAACCCAAATAGGAAGAAGCCACTCATAAGG ATAATGGGTTTATATAATTCACAGCAAAGAAAGCCATCGAACTATTCGATTAATTATCCATTCTTTT TTTTTTTAGTTTGAATGTATAAGAACAAAGAGTTGTTACGCATCATGACAATGTCTTAGAAAACAA AAGAAATGAATAAAAAAGTAAAACGAAAAATAAAAAGTGAGGATGAAGTTGTTGAATGAGTTGG CGAGGCGGCGACTTTTTCATACATTCCATTTACTTAATTCCTAAAGTCCTTCTCACATCTCTTTGTTA TATAATGACACCATAACCATTTCTTCTCTTCACAATCTTTACAAGAATATCTCTCTTCTACAGTAAA CAAAAA >SEQ ID NO: 151 ACGTAAGCTTCTTAGCCAATGGATGAGGATG >SEQ ID NO: 152 ACGTTCTAGATTTTTGTTTACTGTAGAAGAG >SEQ ID NO: 153 TGCTGCTTCAAATCCTTCTATAGCTCCTGTTTATACCACCATGACTACTTTCTCTCCAGGAATTCAAA TGGGAAGTGGTGAAGAACACAGATTAGATGCACATAAGAAACTCCTGATTGGTCTTATAATCAGTT CCTCTTCTCTTGGTATCGTAATCTTGATTTGCTTTGGCTTCTGGATGTACTGTCGCAAGAAAGCTCCC AAACCCATCAAGATTCCGGATGCTGAGAGTGGGACTTCATCATTTTCAATGTTTGTGAGGCGGCTA AGCTCAATCAAAACTCAGAGAACATCTAGCAATCAGGGTTATGTGCAGCGTTTCGATTCCAAGACG CTAG >SEQ ID NO: 154 TATGGATCCTGCTGCTTCAAATCCTTCTATAGCTCCTG >SEQ ID NO: 155 TATTCTAGACTAGCGTCTTGGAATCGAAACGCTGCAC >SEQ ID NO: 156 TATGAGCTCTGCTGCTTCAAATCCTTCTATAGCTCCTG >SEQ ID NO: 157 TATGAGCTCCTAGCGTCTTGGAATCGAAACGCTGCAC >SEQ ID NO: 158 GCAGATCGCTCCTCCCGTCGTGAT >SEQ ID NO: 159 CGCCTAGG AGCGACGGGTACTCGATCAT >SEQ ID NO: 160 CCTAGCTA AGCGACGGGTACTCGATCAT >SEQ ID NO: 161 GCTCCTCCCGTCGTGATCACAGTGGTGAGGCACCACCATTACCACCGGGAGCTGGTCATCTCCGCT GTCCTCGCCTGCGTCGCCACCGCCATGATCCTCCTCTCCACACTCTACGCCTGGACGATGTGGCGGC GGTCTCGCCGGACCCCCCACGGCGGCAAGGGCCGCGGCCGGAGATCAGGGATCACACTGGTGCCA ATCCTGAGCAAGTTCAATTCAGTGAAGATGAGCAGGAAGGGGGGCCTTGTGACGATGATCGAGTA CCCGTCGCT >SEQ ID NO: 162 CGGGATCCCGGCATAACAAACTCGTGCATCC >SEQ ID NO: 163 CCATCGATGGCGCCAAACACAATA GCT CAA >SEQ ID NO: 164 GTAAGTAATTTCAAGTTTAAGTTTCATAAGCATAACAAACTCGTGCATCCAATTTGAACCATTTTAC TGTCCTGGCATCCTCTAAATATTTCCTTGATTATCAGCTTATCTTCATCCCATTGAATCAGAAAATTA CCAACCCTTGTTTTAGCTTTAATCATTGTTATTTGTTGTCTGAGGGGCTACACTGTTTCTTTATATTG GTGAAGGAGTTACCAGGCAAAAATTCCCACCTCCTGATATTAGCAGAGACCCCCTTTTTTGTGCCT GTATGCATACTAACAAATAATACAGATGGAAATATGTATATTTGTTATATCATGGATTGATGCTTTA TGTTTAGCAAGTCCATGCAATGGTAGTCAAAAGATGTAAACTTTTGAATGATATATTGGGGCTTTA GATTAGCCATTTTTACCCTCACTTGAAAATGACAATTTTGCCCTTCCGATCTACTTTCTCTTGTCACC TCAGGCAGGCTCTTGAAAGTTCTTATCCCTGAATTCCGTGGAAGTTTATTATTCTAATGTTATAGTT TACTTAAAGTGTCGCATAATCTACTAGAGCCTAATGGAAGTACTGATGGACTTTGTTTTGCTACAAT CACTGCTTGCAAGAATGACTACTTTGGGGCATTTCTAATATATTATTGATATTTCTATGATGTATTG TTGTCCATGTACTTCAGTCCTTACAGCGACTAGTCCTATTTCTGCATTGATAAATTGTTCACTGTCAG ACCATCTTGAGTGGCAAGAATGAGTATAACATGTCTTGTTTTTCTGTGATTTCAAGGTAAGCGCACA TGCGCACAGTGTACACCGTCACCACATGTGAGTACACCCCCTAGTACACATGTAAAAAAAGCACAG TCCAGTTATTAAATGGACCATTGGCATTGATTGTCGTGTTTATAGGAGTAAAGATACATGTAAACA CTAATTCATTGGGAGATATAAATTTATACTACCATTGAATGTGACATAGGCTCTAAGGTTTTTAGTT CAGCATTTCGAAAGAGCTTTGTTTGGTTGGCTTGGGATGGAATCAGGTGACAACATTTTTGGGTTGC AGCAAATTTAATATTGATTGAGGAGGCATACAACGAAATCATTGAGCTATTGTGTTTGGCGTTACA TCTATGGAATTTCTTCTAATCTGATTATTGTTTGTA >SEQ ID NO: 165 GATCCGCTCCTCCCGTCGTGAT >SEQ ID NO: 166 AACGCGATCGCTTGCATGCCTGCAGTAGAC >SEQ ID NO: 167 GACTTAATTAAGAATTCGAGCTCGGGTA >SEQ ID NO: 168 TCGTAGTGCACCACCATTTCCACCGCGAGCTGGTCATCGCCGCCGTCCTCGCCTGCATCGCCACCGT CACGATCTTCCTTTCCACGCTCTACGCTTGGACACTATGGCGGCGATCTCGCCGGAGCACCGGCGG CAAGGTCACCAGGAGCTCAGACGCAGCGAAGGGGATCAAGCTGGTGCCGATCTTGAGCAGGTTCA ACTCGGTGAAGATGAGCAGGAAGAGGCTGGTTGGGATGTTCGAGTACCCGTCG >SEQ ID NO: 169 GCAGATCTCGTAGTGCACCACCATTTC >SEQ ID NO: 170 CGCCTAGGCGACGGGTACTCGAACATC >SEQ ID NO: 171 CCTAGCTACGACGGGTACTCGAACATC >SEQ ID NO: 172 GATCCTCGTAGTGCACCACCATTTC >SEQ ID NO: 173 CTCGTAGTGCACCACCATTTC >SEQ ID NO: 174 AATGGGACCGCCTCCGTTGCTCCGGCGGTGCCGGCGCCGCCTCCCGTCGTGATCATCGTGGAGCGG CGCCATCATTTCCACCGCGAGCTAGTCATCGCCTCCGTTCTCGCCTCCATCGCCATCGTCGCGATTA TCCTCTCCACGCTCTATGCGTGGATCCTGTGGCGGCGGTCTCGCCGGCTGCCCAGCGGCAAGGGCG CCAGGAGCGCAGACACCGCGAGGGGAATCATGCTGGTGCCGATCCTGAGCAAGTTCCACTCA >SEQ ID NO: 175 GCAGATCAATGGGACCGCCTCCGTTG >SEQ ID NO: 176 CGCCTAGGTGAGTGGAACTTGCTCAGGA >SEQ ID NO: 177 CCTAGCTATGAGTGGAACTTGCTCAGGA >SEQ ID NO: 178 GTAAGTATTCTTGCAACACATTACTATTTTCAATAACCACAAGTTTAAAAGCTTGAGTCCATTTCGC AAACCAGTTGTTCATAACCAAATTCTTAGGTAATTAGGTCCAATTGAGAAAATCTGATCATTGAAC ACTAGCAGGAAATAACTCAGACATAGTTTCTGCATACTATAATGATGCTTAATATATTTGTTCTCTT TTGAGATTGTATTGCATAGACATTTCTGTGTAAAATAATGTTTTACATCATGTATATATATCACTTTT TATAG >SEQ ID NO: 179 CGGGATCCTTCTTGCAACACATTACTATTT >SEQ ID NO: 180 CCATCGATGAAATGTCTATGCAATACAATCTCAA >SEQ ID NO: 181 GATCCAATGGGACCGCCTCCGTTG >SEQ ID NO: 182 CAATGGGACCGCCTCCGTTGA >SEQ ID NO: 183 GGCCCCGGCCGCGCGCGTCTCCGTGTCCTCCGCGACTGTGCACGTTTCGTCGGGAGCGGCGTGCCC ACGCCCACCCCCCGTCCACCAGCCAGCAACCGACGGCACTGGTGACACGCGGCTGGTCCGCTCGGT CCGCCCCGCGGCTCCAGATCACGGCAAGCGCGCCCGCCGCCCGCTGCTGCGCTGCGCTGCACGTCC CGCCCTGACGCCACGCCACGCCAAGCGCGACACGACACGACACGACACGACCCGACCCCCGCCAA CGAAACGCCGAAACGCGGCAACGCGTGACGGGCGCGCATGGTCGATGCTCTACCCGCGCGTCCGC CCCACGCCAATCTCCCGGCGGGTCCCTCGTGGGACGGGGAACGCGATGCGGCTGCAGGCTGCGAC CGCGACCGCGACCGCGACCGCGCCCACGTGAAGGCAGGCAGGCAGCCCCGGAGCGGGCGCGGCG GTGGGCCAACGACGCGTTGCCGTCGCGAATCTTCTTCTGGCCACGGCCAAGGGCCAATCGCCCGCT CCGCTCCGCTCCGCACTCCGCCTCCGCTAGGGAATATGGAACCCGATCCCACGGCCCTCTGGGTCT GGTCGACGGGTCCTCTCGCCGTGGCAGCTGCTTCCCGGACCGGAGGATCGCTGAGCGCGGACGCCA CTGCCATTGCCGTCCGACTATAGTTGTTAATTACCATAAAATAATTTGTTAACGATAAAACCCGTGT CAGGCACCGTCGTCTGGACGCTGCTATGGGATAACCATTCGCGTACGTCGGTTGTATGGGTGGGAT CCTCTGCGGCACGCCATTCTGGTGCTGCTAGTGGAATAGACAAAAAAAGGGCCGACGGTGTTTGCT CGTGGCAGGCCACACAGAGTGACAACCAGAGTGGTTGCCGCAAAAACAACCAATCACACAAAAAG TGTTGTACCGGTGGAGGACAGCCATTAATCAGCAGGCCGGCTTCGCGGCCAAAAGAAACGGAGAA GAGGAAAAAGGGGGGC >SEQ ID NO: 184 TCCCAAGCTTGCGCGTCTCCGTGTCCTC >SEQ ID NO: 185 AGTAAAGCTTCCCCCTTTTTCCTCTTCTCC >SEQ ID NO: 186 TAATGGTCGAGTGAGGCCCGTATAGATGTAGTTAAATAGCTAAAATTTTTGGAGAAATAAGCATTT TTTTGGAAGAATATATTTAAACATGGGCTTGTAAAACTTGGCTGTAAAGATTTGGAATTTAGGATCT TGGAGCCCCAAAACTGTATAAACTTGCTTAGGGACCCGTGTCTTGTGTGTTGCAGACCAAAAAATT TAGAAAGCATCTAAACACCTATTTGAATGTAAAGTTTACAGCCAAAAGTTTTAGGATGTAAAGATT TGGGATCTAAAAGTAGTCATTAGGAAATAACACGTTAGAGAGAGAGAGTAGATCTTCTTATTGGTT TCTCATGCACTAATCGAACCAATCACTGGACCACTTGAACCAAACTTTATCACATTGAACTTTGTCA GTTCAGTTCGAACGCAGGACTGGAGCTGCCCTTAAGGCCAATTGCTCAAGATTCATTCAACAATTG AAACATCTCCCATGATTAAATCAGTATAAGGTTGCTATGGTCTTGCTTGACAAAGTTTTTTTTTTGA GGGAATTTCAACTAAATTTTTGAGTGAAACTATCAAATACTGATTTTAAAAATTTTTTATAAAAGGA AGCGCAGAGATAAAAGGCCATCTATGCTACAAAAGTACCCAAAAATGTAATCCTAAAGTATGAAT TGCATTTTTTTTGTTTGGACGAAAGGAAAGGAGTATTACCACAAGAATGATATCATCTTCATATTTA GATCTTTTTTGGGTAAAGCTTGAGATTCTCTAAATATAGAGAAATCAGAAGAAAAAAAAACCGTGT TTTGGTGGTTTTGATTTCTAGCCTCCACAATAACTTTGACGGCGTCGACAAGTCTAACGGACACCAA GCAGCGAACCACCAGCGCCGAGCCAAGCGAAGCAGACGGCCGAGACGTTGACACCTTCGGCGCGG CATCTCTCGAGAGTTCCGCTCCGGCGCTCCACCTCCACCGCTGGCGGTTTCTTATTCCGTTCCGTTCC GCCT >SEQ ID NO: 187 AACTGCAGGGTCGAGTGAGGCCCGTA

>SEQ ID NO: 188 TTCTGCAGGGAACGGAACGGAATAAGAA >SEQ ID NO: 189 GCCGTGGGTCGTTTAAGCTGCCGCTGTACCTGTGTCGTCTGGTGCCTTCTGGTGTACCTGGGAGGTT GTCGTCTATCAAGTATCTGTGGTTGGTGTCATGAGTCAGTGAGTCCCAATACTGTTCGTGTCCTGTG TGCATTATACCCAAAACTGTTATGGGCAAATCATGAATAAGCTTGATGTTCGAACTTAAAAGTCTC TGCTCAATATGGTATTATGGTTGTTTTTGTTCGTCTCCT >SEQ ID NO: 190 TAGGTACCGCCGTGGGTCGTTTAAGCT >SEQ ID NO: 191 AAGGTACCAGGAGACGAACAAAAACAA >SEQ ID NO: 192 AACGCGATCGTAATGGTCGAGTGAGGCCCGTATA >SEQ ID NO: 193 ATGAAGAAACTGGTTCATCTTCAGTTTCTGTTTCTTGTCAAGATCTTTGCTACTCAATTC CTCACTCCTTCTTCATCATCTTTTGCTGCTTCAAATCCTTCTATAGCTCCTGTTTATACC ACCATGACTACTTTCTCTCCAGGAATTCAAATGGGAAGTGGTGAAGAACACAGATTAGAT GCACATAAGAAACTCCTGATTGGTCTTATAATCAGTTCCTCTTCTCTTGGTATCGTAATC TTGATTTGCTTTGGCTTCTGGATGTACTGTCGCAAGAAAGCTCCCAAACCCATCAAGATT CCGGATGCTGAGAGTGGGACTTCATCATTTTCAATGTTTGTGAGGCGGCTAAGCTCAATC AAAACTCAGAGAACATCTAGCAATCAGGGTTATGTGCAGCGTTTCGATTCCAAGACGCTA GAGAAAGCGACAGGCGGTTTCAAAGACAGTAATGTAATCGGACAGGGCGGTTTCGGATGC GTTTACAAGGCTTCTTTGGACAGCAACACTAAAGCAGCGGTTAAAAAGATCGAAAACGTT AGCCAAGAAGCAAAACGAGAATTTCAGAATGAAGTTGAGCTGTTGAGCAAGATCCAGCAC TCCAATATTATATCATTGTTGGGCTCTGCAAGTGAAATCAACTCGAGTTTCGTCGTTTAT GAGTTGATGGAGAAAGGATCCTTAGATGATCAGTTACATGGACCTTCGTGTGGATCCGCT CTAACATGGCATATGCGTATGAAGATTGCTCTAGATACAGCTAGAGGATTAGAGTATCTC CATGAACATTGTCGTCCACCAGTTATCCACAGGGACCTGAAATCGTCTAATATACTTCTT GATTCTTCCTTCAATGCCAAGATTTCAGATTTTGGTCTGGCTGTATCGGTTGGAGTGCAT GGGAGTAACAACATTAAACTCTCTGGGACACTTGGTTATGTTGCCCCGGAATATCTCCTA GACGGAAAGTTGACGGATAAGAGTGATGTCTATGCATTTGGGGTGGTTCTTCTTGAACTT TTGTTGGGTAGAAGGCCGGTTGAGAAATTGAGTCCATCTCAGTGTCAATCTCTTGTGACT TGGGCAATGCCACAACTTACCGATAGATCGAAACTCCCAAACATCGTGGATCCGGTTATA AAAGATACAATGGATCTTAAGCACTTATACCAGGTAGCAGCCATGGCTGTGTTGTGCGTT CAGCCAGAACCGAGTTACCGGCCGCTGATAACCGATGTTCTTCACTCACTTGTTCCATTG GTTCCGGTCGAACTAGGAGGGACTCTCCGGTTAACCCGATGA >SEQ ID NO: 194 MKKLVHLQFLFLVKIFATQFLTPSSSSFAASNPSIAPVYTTMTTFSPGIQMGSGEEHRLD AHKKLLIGLIISSSSLGIVILICFGFWMYCRKKAPKPIKIPDAESGTSSFSAVVRRLSSI KTQRTSSNQGYVQRFDSKTLEKATGGFKDSNVIGQGGFGCVYKASLDSNTKAAVKKIENV SQEAKREFQNEVELLSKIQHSNITSLLGSASEINSSFVVYELMEKGSLDDQLHGPSCGSA LTWHMRMKIALDTARGLEYLHEHCRPPVIHRDLKSSNILLDSSFNAKISDFGLAVSVGVH GSNNIKLSGTLGYVAPEYLLDGKLTDKSDVYAFGVVLLELLLGRRPVEKLSPSQCQSLVT WAMPQLTDRSKLPNIVDPVIKDTMDLKHLYQVAAMAVLCVQPEPSYRPLITDVLHSLVPL VPVELGGTLRLTR >SEQ ID NO: 195 AATCCAGCTCATTCTGGAATTCCTTCTCGCA >SEQ ID NO: 196 TGAACTTGCTCAGGATTGGCACCAGTGTGATC >SEQ ID NO: 197 MEIPAAPPPPLPVLCSYVVFLLLLSSCSLARGRIAVSSPGPSPVAAAVTANETASSSSSP VFPAAPPVVITVVRHHHYHRELVISAVLACVATAMILLSTLYAWTMWRRSRRTPHGGKGR GRRSGITLVPILSKFNSVKMSRKGGLVTMIEYPSLEAATGKFGESNVLGVGGFGCVYKAA FDGGATAAVKRLEGGGPDCEKEFENELDLLGRIRHPNIVSLLGFCVHGGNHYIVYELMEK GSLETQLHGSSHGSALSWHVRMKIALDTARGLEYLHEHCNPPVIHRDLKPSNILLDSDFN AKIADFGLAVTGGNLNKGNLKLSGTLGYVAPEYLLDGKLTEKSDVYAFGVVLLELLMGRK PVEKMSPSQCQSIVSWAMPQLTDRSKLPNIIDLVIKDTMDPKHLYQVAAVAVLCVQPEPS YRPLITDVLHSLVPLVPAELGGTLRVAEPPSPSPDQRHYPC >SEQ ID NO: 198 TATACCGGTAAAATGAGAGAGCTTCTTCTTCTTCTTCTTCTTCATTTTCAGTC >SEQ ID NO: 199 ATATACCGGTCTTGTTAACCGGAGAGTCCCTCCTAGCTC >SEQ ID NO: 200 CGCTCCTCCCGTCGTGAT

LITERATURE

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[0212] 26. Kado C I, Hooykaas P J (1991) Molecular mechanisms of crown gall tumorigenesis. Crit. Rev. Plant Sci. 10: 1-32.

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[0214] 28. Keil M, Sanchez-Serrano J, Schell J, Willmitzer L (1986) Primary structure of a proteinase inhibitor II gene from potato (Solanum tuberosum). Nucl. Acids Res. 14: 5641-5650.

[0215] 29. Laemmli, U K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227(259): 680-685.

[0216] 30. Last D I, Brettell R I, Chamberlain D A, Chaudhury A M, Larkin P J, Marsh E L, Peacock W J, Dennis E S (1991) pEmu: an improved promoter for gene expression in cereal cells Theor. Appl. Genet. 81: 581-588.

[0217] 31. Lepetit M, Ehling M, Chaubet N, Gigot C (1992) A plant histone gene promoter can direct both replication-dependent and -independent gene expression in transgenic plants. Mol. Gen. Genet., 231: 276-285.

[0218] 32. Lund P, Dunsmuir P (1992) A plant signal sequence enhances the secretion of bacterial ChiA in transgenic tobacco. Plant Mol. Biol. 18: 47-53.

[0219] 33. McElroy D, Zhang W, Cao J, Wu R (1990) Isolation of an efficient actin promoter for use in rice transformation. Plant Cell 2(2): 163-171.

[0220] 34. Mian MAR, Bailey M A, Ashley D A, Wells R, Carter T E Jr, Parrott W A, Boerma H R (1996) Molecular markers associated with water use efficiency and leaf ash in soybean. Crop Sci. 36: 1252-1257.

[0221] 35. Martin B, Nienhuis J, King G, Schaefer A (1989) Restriction Fragment Length Polymorphisms Associated with Water Use Efficiency in Tomato. Science. 243(4899):1725-1728.

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[0223] 37. Matsuoka K, Nakamura K (1991) Propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting. Proc. Nat'l Acad. Sci. USA 88: 834-838.

[0224] 38. van der Meer I M, Spelt C E, Mol J N, Stuitje A R (1990) Promoter analysis of the chalcone synthase (chsA) gene of Petunia hybrida: a 67 bp promoter region directs flower-specific expression. Plant Molecular Biology 15(1): 95-109.

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Sequence CWU 1

1

20011299DNAARABIDOPSIS THALIANA 1atgagagagc ttcttcttct tcttcttctt cattttcagt ctctaattct tttgatgatc 60ttcatcactg tctctgcttc ttctgcttca aatccttctt tagctcctgt ttactcttcc 120atggctacat tctctcctcg aatccaaatg ggaagtggtg aagaagatag atttgatgct 180cataagaaac ttctgattgg tctcataatc agtttctctt ctcttggcct tataatcttg 240ttctgttttg gcttttgggt ttatcgcaag aaccaatctc caaaatccat caacaactca 300gattctgaga gtgggaattc attttccttg ttaatgagac gacttggctc gattaaaact 360cagagaagaa cttctatcca aaagggttac gtgcaatttt tcgatatcaa gaccctcgag 420aaagcgacag gcggttttaa agaaagtagt gtaatcggac aaggcggttt cggatgcgtt 480tacaagggtt gtttggacaa taacgttaaa gcagcggtca agaagatcga gaacgttagc 540caagaagcaa aacgagaatt tcagaatgaa gttgacttgt tgagcaagat ccatcactcg 600aacgttatat cattgttggg ctctgcaagc gaaatcaact cgagtttcat cgtttatgag 660cttatggaga aaggatcatt agatgaacag ttacatgggc cttctcgtgg atcagctcta 720acatggcaca tgcgtatgaa gattgctctt gatacagcta gaggactaga gtatctccat 780gagcattgtc gtccaccagt tatccacaga gatttgaaat cttcgaatat tcttcttgat 840tcttccttca acgccaagat ttcagatttc ggtcttgctg tatcgctgga tgaacatggc 900aagaacaaca ttaaactctc tgggacactt ggttatgttg ccccggaata cctccttgac 960ggaaaactga cggataagag tgatgtttat gcatttgggg tagttctgct tgaactcttg 1020ttgggtagac gaccagttga aaaattaact ccagctcaat gccaatctct tgtaacttgg 1080gcaatgccac aacttaccga tagatccaag cttccaaaca ttgtggatgc cgttataaaa 1140gatacaatgg atctcaaaca cttataccag gtagcagcca tggctgtgtt gtgcgtgcag 1200ccagaaccaa gttaccggcc gttgataacc gatgttcttc actcacttgt tccactggtt 1260ccggtagagc taggagggac tctccggtta acaagatga 12992432PRTARABIDOPSIS THALIANA 2Met Arg Glu Leu Leu Leu Leu Leu Leu Leu His Phe Gln Ser Leu Ile1 5 10 15Leu Leu Met Ile Phe Ile Thr Val Ser Ala Ser Ser Ala Ser Asn Pro 20 25 30Ser Leu Ala Pro Val Tyr Ser Ser Met Ala Thr Phe Ser Pro Arg Ile 35 40 45Gln Met Gly Ser Gly Glu Glu Asp Arg Phe Asp Ala His Lys Lys Leu 50 55 60Leu Ile Gly Leu Ile Ile Ser Phe Ser Ser Leu Gly Leu Ile Ile Leu65 70 75 80Phe Cys Phe Gly Phe Trp Val Tyr Arg Lys Asn Gln Ser Pro Lys Ser 85 90 95Ile Asn Asn Ser Asp Ser Glu Ser Gly Asn Ser Phe Ser Leu Leu Met 100 105 110Arg Arg Leu Gly Ser Ile Lys Thr Gln Arg Arg Thr Ser Ile Gln Lys 115 120 125Gly Tyr Val Gln Phe Phe Asp Ile Lys Thr Leu Glu Lys Ala Thr Gly 130 135 140Gly Phe Lys Glu Ser Ser Val Ile Gly Gln Gly Gly Phe Gly Cys Val145 150 155 160Tyr Lys Gly Cys Leu Asp Asn Asn Val Lys Ala Ala Val Lys Lys Ile 165 170 175Glu Asn Val Ser Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val Asp 180 185 190Leu Leu Ser Lys Ile His His Ser Asn Val Ile Ser Leu Leu Gly Ser 195 200 205Ala Ser Glu Ile Asn Ser Ser Phe Ile Val Tyr Glu Leu Met Glu Lys 210 215 220Gly Ser Leu Asp Glu Gln Leu His Gly Pro Ser Arg Gly Ser Ala Leu225 230 235 240Thr Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu 245 250 255Glu Tyr Leu His Glu His Cys Arg Pro Pro Val Ile His Arg Asp Leu 260 265 270Lys Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile Ser 275 280 285Asp Phe Gly Leu Ala Val Ser Leu Asp Glu His Gly Lys Asn Asn Ile 290 295 300Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp305 310 315 320Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu 325 330 335Leu Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Thr Pro Ala 340 345 350Gln Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg 355 360 365Ser Lys Leu Pro Asn Ile Val Asp Ala Val Ile Lys Asp Thr Met Asp 370 375 380Leu Lys His Leu Tyr Gln Val Ala Ala Met Ala Val Leu Cys Val Gln385 390 395 400Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu 405 410 415Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Thr Arg 420 425 43031299DNAARABIDOPSIS THALIANA 3atgagagagc ttcttcttct tcttcttctt cattttcagt ctctaattct tttgatgatc 60ttcatcactg tctctgcttc ttctgcttca aatccttctt tagctcctgt ttactcttcc 120atggctacat tctctcctcg aatccaaatg ggaagtggtg aagaagatag atttgatgct 180cataagaaac ttctgattgg tctcataatc agtttctctt ctcttggcct tataatcttg 240ttctgttttg gcttttgggt ttatcgcaag aaccaatctc caaaatccat caacaactca 300gattctgaga gtgggaattc attttccttg ttaatgagac gacttggctc gattaaaact 360cagagaagaa cttctatcca aaagggttac gtgcaatttt tcgatatcaa gaccctcgag 420aaagcgacag gcggttttaa agaaagtagt gtaatcggac aaggcggttt cggatgcgtt 480tacaagggtt gtttggacaa taacgttaaa gcagcggtca agaagatcga gaacgttagc 540caagaagcaa aacgagaatt tcagaatgaa gttgacttgt tgagcaagat ccatcactcg 600aacgttatat cattgttggg ctctgcaagc gaaatcaact cgagtttcat cgtttatgag 660cttatggaga aaggatcatt agatgaacag ttacatgggc cttctcgtgg atcagctcta 720acatggcaca tgcgtatgaa gattgctctt gatacagcta gaggactaga gtatctccat 780gagcattgtc gtccaccagt tatccacaga gatttgaaat cttcgaatat tcttcttgat 840tcttccttca acgccaagat ttcagatttc ggttttgctg tatcgctgga tgaacatggc 900aagaacaaca ttaaactctc tgggacactt ggttatgttg ccccggaata cctccttgac 960ggaaaactga cggataagag tgatgtttat gcatttgggg tagttctgct tgaactcttg 1020ttgggtagac gaccagttga aaaattaact ccagctcaat gccaatctct tgtaacttgg 1080gcaatgccac aacttaccga tagatccaag cttccaaaca ttgtggatgc cgttataaaa 1140gatacaatgg atctcaaaca cttataccag gtagcagcca tggctgtgtt gtgcgtgcag 1200ccagaaccaa gttaccggcc gttgataacc gatgttcttc actcacttgt tccactggtt 1260ccggtagagc taggagggac tctccggtta acaagatga 12994432PRTARABIDOPSIS THALIANA 4Met Arg Glu Leu Leu Leu Leu Leu Leu Leu His Phe Gln Ser Leu Ile1 5 10 15Leu Leu Met Ile Phe Ile Thr Val Ser Ala Ser Ser Ala Ser Asn Pro 20 25 30Ser Leu Ala Pro Val Tyr Ser Ser Met Ala Thr Phe Ser Pro Arg Ile 35 40 45Gln Met Gly Ser Gly Glu Glu Asp Arg Phe Asp Ala His Lys Lys Leu 50 55 60Leu Ile Gly Leu Ile Ile Ser Phe Ser Ser Leu Gly Leu Ile Ile Leu65 70 75 80Phe Cys Phe Gly Phe Trp Val Tyr Arg Lys Asn Gln Ser Pro Lys Ser 85 90 95Ile Asn Asn Ser Asp Ser Glu Ser Gly Asn Ser Phe Ser Leu Leu Met 100 105 110Arg Arg Leu Gly Ser Ile Lys Thr Gln Arg Arg Thr Ser Ile Gln Lys 115 120 125Gly Tyr Val Gln Phe Phe Asp Ile Lys Thr Leu Glu Lys Ala Thr Gly 130 135 140Gly Phe Lys Glu Ser Ser Val Ile Gly Gln Gly Gly Phe Gly Cys Val145 150 155 160Tyr Lys Gly Cys Leu Asp Asn Asn Val Lys Ala Ala Val Lys Lys Ile 165 170 175Glu Asn Val Ser Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val Asp 180 185 190Leu Leu Ser Lys Ile His His Ser Asn Val Ile Ser Leu Leu Gly Ser 195 200 205Ala Ser Glu Ile Asn Ser Ser Phe Ile Val Tyr Glu Leu Met Glu Lys 210 215 220Gly Ser Leu Asp Glu Gln Leu His Gly Pro Ser Arg Gly Ser Ala Leu225 230 235 240Thr Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu 245 250 255Glu Tyr Leu His Glu His Cys Arg Pro Pro Val Ile His Arg Asp Leu 260 265 270Lys Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile Ser 275 280 285Asp Phe Gly Phe Ala Val Ser Leu Asp Glu His Gly Lys Asn Asn Ile 290 295 300Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp305 310 315 320Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu 325 330 335Leu Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Thr Pro Ala 340 345 350Gln Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg 355 360 365Ser Lys Leu Pro Asn Ile Val Asp Ala Val Ile Lys Asp Thr Met Asp 370 375 380Leu Lys His Leu Tyr Gln Val Ala Ala Met Ala Val Leu Cys Val Gln385 390 395 400Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu 405 410 415Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Thr Arg 420 425 43051160DNAARABIDOPSIS THALIANA 5atgggaagtg gtgaagaaga tagatttgat gctcataaga aacttctgat tggtctcata 60atcagtttct cttctcttgg ccttataatc ttgttctgtt ttggcttttg ggtttatcgc 120aagaaccaat ctccaaaatc catcaacaac tcagattctg agagtgggaa ttcattttcc 180ttgttaatga gacgacttgg ctcgattaaa actcagagaa gaacttctat ccaaaagggt 240tacgtgcaat ttttcgatat caagaccctc gagaaagcga caggcggttt taaagaaagt 300agtgtaatcg gacaaggcgg tttcggatgc gtttacaagg gttgtttgga caataacgtt 360aaagcagcgg tcaagaagat cgagaacgtt agccaagaag caaaacgaga atttcagaat 420gaagttgact tgttgagcaa gatccatcac tcgaacgtta tatcattgtt gggctctgca 480agcgaaatca actcgagttt catcgtttat gagcttatgg agaaaggatc attagatgaa 540cagttacatg ggccttctcg tggatcagct ctaacatggc acatgcgtat gaagattgct 600cttgatacag ctagaggact agagtatctc catgagcatt gtcgtccacc agttatccac 660agagatttga aatcttcgaa tattcttctt gattcttcct tcaacgccaa gatttcagat 720ttcggttttg ctgtatcgct ggatgaacat ggcaagaaca acattaaact ctctgggaca 780cttggttatg ttgccccgga atacctcctt gacggaaaac tgacggataa gagtgatgtt 840tatgcatttg gggtagttct gcttgaactc ttgttgggta gacgaccagt tgaaaaatta 900actccagctc aatgccaatc tcttgtaact tgggcaatgc cacaacttac cgatagatcc 960aagcttccaa acattgtgga tgccgttata aaagatacaa tggatctcaa acacttatac 1020caggtagcag ccatggctgt gttgtgcgtg cagccagaac caagttaccg gccgttgata 1080accgatgttc ttcactcact tgttccactg gttccggtag agctaggagg gactctccgg 1140ttaacaagat gattcacaga 11606383PRTARABIDOPSIS THALIANA 6Met Gly Ser Gly Glu Glu Asp Arg Phe Asp Ala His Lys Lys Leu Leu1 5 10 15Ile Gly Leu Ile Ile Ser Phe Ser Ser Leu Gly Leu Ile Ile Leu Phe 20 25 30Cys Phe Gly Phe Trp Val Tyr Arg Lys Asn Gln Ser Pro Lys Ser Ile 35 40 45Asn Asn Ser Asp Ser Glu Ser Gly Asn Ser Phe Ser Leu Leu Met Arg 50 55 60Arg Leu Gly Ser Ile Lys Thr Gln Arg Arg Thr Ser Ile Gln Lys Gly65 70 75 80Tyr Val Gln Phe Phe Asp Ile Lys Thr Leu Glu Lys Ala Thr Gly Gly 85 90 95Phe Lys Glu Ser Ser Val Ile Gly Gln Gly Gly Phe Gly Cys Val Tyr 100 105 110Lys Gly Cys Leu Asp Asn Asn Val Lys Ala Ala Val Lys Lys Ile Glu 115 120 125Asn Val Ser Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val Asp Leu 130 135 140Leu Ser Lys Ile His His Ser Asn Val Ile Ser Leu Leu Gly Ser Ala145 150 155 160Ser Glu Ile Asn Ser Ser Phe Ile Val Tyr Glu Leu Met Glu Lys Gly 165 170 175Ser Leu Asp Glu Gln Leu His Gly Pro Ser Arg Gly Ser Ala Leu Thr 180 185 190Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu Glu 195 200 205Tyr Leu His Glu His Cys Arg Pro Pro Val Ile His Arg Asp Leu Lys 210 215 220Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile Ser Asp225 230 235 240Phe Gly Phe Ala Val Ser Leu Asp Glu His Gly Lys Asn Asn Ile Lys 245 250 255Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly 260 265 270Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu 275 280 285Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Thr Pro Ala Gln 290 295 300Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser305 310 315 320Lys Leu Pro Asn Ile Val Asp Ala Val Ile Lys Asp Thr Met Asp Leu 325 330 335Lys His Leu Tyr Gln Val Ala Ala Met Ala Val Leu Cys Val Gln Pro 340 345 350Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu Val 355 360 365Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Thr Arg 370 375 38071160DNAARABIDOPSIS THALIANA 7atgggaagtg gtgaagaaga tagatttgat gctcataaga aacttctgat tggtctcata 60atcagtttct cttctcttgg ccttataatc ttgttctgtt ttggcttttg ggtttatcgc 120aagaaccaat ctccaaaatc catcaacaac tcagattctg agagtgggaa ttcattttcc 180ttgttaatga gacgacttgg ctcgattaaa actcagagaa gaacttctat ccaaaagggt 240tacgtgcaat ttttcgatat caagaccctc gagaaagcga caggcggttt taaagaaagt 300agtgtaatcg gacaaggcgg tttcggatgc gtttacaagg gttgtttgga caataacgtt 360aaagcagcgg tcaagaagat cgagaacgtt agccaagaag caaaacgaga atttcagaat 420gaagttgact tgttgagcaa gatccatcac tcgaacgtta tatcattgtt gggctctgca 480agcgaaatca actcgagttt catcgtttat gagcttatgg agaaaggatc attagatgaa 540cagttacatg ggccttctcg tggatcagct ctaacatggc acatgcgtat gaagattgct 600cttgatacag ctagaggact agagtatctc catgagcatt gtcgtccacc agttatccac 660agagatttga aatcttcgaa tattcttctt gattcttcct tcaacgccaa gatttcagat 720ttcggtcttg ctgtatcgct ggatgaacat ggcaagaaca acattaaact ctctgggaca 780cttggttatg ttgccccgga atacctcctt gacggaaaac tgacggataa gagtgatgtt 840tatgcatttg gggtagttct gcttgaactc ttgttgggta gacgaccagt tgaaaaatta 900actccagctc aatgccaatc tcttgtaact tgggcaatgc cacaacttac cgatagatcc 960aagcttccaa acattgtgga tgccgttata aaagatacaa tggatctcaa acacttatac 1020caggtagcag ccatggctgt gttgtgcgtg cagccagaac caagttaccg gccgttgata 1080accgatgttc ttcactcact tgttccactg gttccggtag agctaggagg gactctccgg 1140ttaacaagat gattcacaga 11608383PRTARABIDOPSIS THALIANA 8Met Gly Ser Gly Glu Glu Asp Arg Phe Asp Ala His Lys Lys Leu Leu1 5 10 15Ile Gly Leu Ile Ile Ser Phe Ser Ser Leu Gly Leu Ile Ile Leu Phe 20 25 30Cys Phe Gly Phe Trp Val Tyr Arg Lys Asn Gln Ser Pro Lys Ser Ile 35 40 45Asn Asn Ser Asp Ser Glu Ser Gly Asn Ser Phe Ser Leu Leu Met Arg 50 55 60Arg Leu Gly Ser Ile Lys Thr Gln Arg Arg Thr Ser Ile Gln Lys Gly65 70 75 80Tyr Val Gln Phe Phe Asp Ile Lys Thr Leu Glu Lys Ala Thr Gly Gly 85 90 95Phe Lys Glu Ser Ser Val Ile Gly Gln Gly Gly Phe Gly Cys Val Tyr 100 105 110Lys Gly Cys Leu Asp Asn Asn Val Lys Ala Ala Val Lys Lys Ile Glu 115 120 125Asn Val Ser Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val Asp Leu 130 135 140Leu Ser Lys Ile His His Ser Asn Val Ile Ser Leu Leu Gly Ser Ala145 150 155 160Ser Glu Ile Asn Ser Ser Phe Ile Val Tyr Glu Leu Met Glu Lys Gly 165 170 175Ser Leu Asp Glu Gln Leu His Gly Pro Ser Arg Gly Ser Ala Leu Thr 180 185 190Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu Glu 195 200 205Tyr Leu His Glu His Cys Arg Pro Pro Val Ile His Arg Asp Leu Lys 210 215 220Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile Ser Asp225 230 235 240Phe Gly Leu Ala Val Ser Leu Asp Glu His Gly Lys Asn Asn Ile Lys 245 250 255Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly 260 265 270Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu 275 280 285Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Thr Pro Ala Gln 290 295 300Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser305 310 315 320Lys Leu Pro Asn Ile Val Asp Ala Val Ile Lys Asp Thr Met Asp Leu 325 330 335Lys His Leu Tyr Gln Val Ala Ala Met Ala Val Leu Cys Val Gln Pro 340 345 350Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu Val 355

360 365Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Thr Arg 370 375 38091542DNAARABIDOPSIS THALIANA 9atcaaaaact tttcttttct tagcaaaaaa aacaaaaaaa tgagagagct tcttcttctt 60cttcttcttc attttcagtc tctaattctt ttgatgatct tcatcactgt ctctgcttct 120tctgcttcaa atccttcttt agctcctgtt tactcttcca tggctacatt ctctcctcga 180atccaaatgg gaagtggtga agaagataga tttgatgctc ataagaaact tctgattggt 240ctcataatca gtttctcttc tcttggcctt ataatcttgt tctgttttgg cttttgggtt 300tatcgcaaga accaatctcc aaaatccatc aacaactcag attctgagag tgggaattca 360ttttccttgt taatgagacg acttggctcg attaaaactc agagaagaac ttctatccaa 420aagggttacg tgcaattttt cgatatcaag accctcgaga aagcgacagg cggttttaaa 480gaaagtagtg taatcggaca aggcggtttc ggatgcgttt acaagggttg tttggacaat 540aacgttaaag cagcggtcaa gaagatcgag aacgttagcc aagaagcaaa acgagaattt 600cagaatgaag ttgacttgtt gagcaagatc catcactcga acgttatatc attgttgggc 660tctgcaagcg aaatcaactc gagtttcatc gtttatgagc ttatggagaa aggatcatta 720gatgaacagt tacatgggcc ttctcgtgga tcagctctaa catggcacat gcgtatgaag 780attgctcttg atacagctag aggactagag tatctccatg agcattgtcg tccaccagtt 840atccacagag atttgaaatc ttcgaatatt cttcttgatt cttccttcaa cgccaagatt 900tcagatttcg gtcttgctgt atcgctggat gaacatggca agaacaacat taaactctct 960gggacacttg gttatgttgc cccggaatac ctccttgacg gaaaactgac ggataagagt 1020gatgtttatg catttggggt agttctgctt gaactcttgt tgggtagacg accagttgaa 1080aaattaactc cagctcaatg ccaatctctt gtaacttggg caatgccaca acttaccgat 1140agatccaagc ttccaaacat tgtggatgcc gttataaaag atacaatgga tctcaaacac 1200ttataccagg tagcagccat ggctgtgttg tgcgtgcagc cagaaccaag ttaccggccg 1260ttgataaccg atgttcttca ctcacttgtt ccactggttc cggtagagct aggagggact 1320ctccggttaa caagatgatt cacagaaaca cgccaaaaga aatccaaagc catttagatg 1380attttctttt atcctttgcc tttatatttt tttgtatagg gttatgatcc actcatctga 1440aagtttgggg gtaagaatgt gagaatataa gttttcaggg ttgttgagtt ctatataatt 1500atatttgttt ctttttattg tcaaatataa ttatattttt gt 1542101309DNAARABIDOPSIS THALIANA 10aaaatgagag agcttcttct tcttcttctt cttcattttc agtctctaat tcttttgatg 60atcttcatca ctgtctctgc ttcttctgct tcaaatcctt ctttagctcc tgtttactct 120tccatggcta cattctctcc tcgaatccaa atgggaagtg gtgaagaaga tagatttgat 180gctcataaga aacttctgat tggtctcata atcagtttct cttctcttgg ccttataatc 240ttgttctgtt ttggcttttg ggtttatcgc aagaaccaat ctccaaaatc catcaacaac 300tcagattctg agagtgggaa ttcattttcc ttgttaatga gacgacttgg ctcgattaaa 360actcagagaa gaacttctat ccaaaagggt tacgtgcaat ttttcgatat caagaccctc 420gagaaagcga caggcggttt taaagaaagt agtgtaatcg gacaaggcgg tttcggatgc 480gtttacaagg gttgtttgga caataacgtt aaagcagcgg tcaagaagat cgagaacgtt 540agccaagaag caaaacgaga atttcagaat gaagttgact tgttgagcaa gatccatcac 600tcgaacgtta tatcattgtt gggctctgca agcgaaatca actcgagttt catcgtttat 660gagcttatgg agaaaggatc attagatgaa cagttacatg ggccttctcg tggatcagct 720ctaacatggc acatgcgtat gaagattgct cttgatacag ctagaggact agagtatctc 780catgagcatt gtcgtccacc agttatccac agagatttga aatcttcgaa tattcttctt 840gattcttcct tcaacgccaa gatttcagat ttcggtcttg ctgtatcgct ggatgaacat 900ggcaagaaca acattaaact ctctgggaca cttggttatg ttgccccgga atacctcctt 960gacggaaaac tgacggataa gagtgatgtt tatgcatttg gggtagttct gcttgaactc 1020ttgttgggta gacgaccagt tgaaaaatta actccagctc aatgccaatc tcttgtaact 1080tgggcaatgc cacaacttac cgatagatcc aagcttccaa acattgtgga tgccgttata 1140aaagatacaa tggatctcaa acacttatac caggtagcag ccatggctgt gttgtgcgtg 1200cagccagaac caagttaccg gccgttgata accgatgttc ttcactcact tgttccactg 1260gttccggtag agctaggagg gactctccgg ttaacaagat gattcacag 1309111177DNAARABIDOPSIS THALIANA 11tctgtgtcag gaatccaaat gggaagtggt gaagaagata gatttgatgc tcataagaaa 60cttctgattg gtctcataat cagtttctct tctcttggcc ttataatctt gttctgtttt 120ggcttttggg tttatcgcaa gaaccaatct ccaaaatcca tcaacaactc agattctgag 180agtgggaatt cattttcctt gttaatgaga cgacttggct cgattaaaac tcagagaaga 240acttctatcc aaaagggtta cgtgcaattt ttcgatatca agaccctcga gaaagcgaca 300ggcggtttta aagaaagtag tgtaatcgga caaggcggtt tcggatgcgt ttacaagggt 360tgtttggaca ataacgttaa agcagcggtc aagaagatcg agaacgttag ccaagaagca 420aaacgagaat ttcagaatga agttgacttg ttgagcaaga tccatcactc gaacgttata 480tcattgttgg gctctgcaag cgaaatcaac tcgagtttca tcgtttatga gcttatggag 540aaaggatcat tagatgaaca gttacatggg ccttctcgtg gatcagctct aacatggcac 600atgcgtatga agattgctct tgatacagct agaggactag agtatctcca tgagcattgt 660cgtccaccag ttatccacag agatttgaaa tcttcgaata ttcttcttga ttcttccttc 720aacgccaaga tttcagattt cggtcttgct gtatcgctgg atgaacatgg caagaacaac 780attaaactct ctgggacact tggttatgtt gccccggaat acctccttga cggaaaactg 840acggataaga gtgatgttta tgcatttggg gtagttctgc ttgaactctt gttgggtaga 900cgaccagttg aaaaattaac tccagctcaa tgccaatctc ttgtaacttg ggcaatgcca 960caacttaccg atagatccaa gcttccaaac attgtggatg ccgttataaa agatacaatg 1020gatctcaaac acttatacca ggtagcagcc atggctgtgt tgtgcgtgca gccagaacca 1080agttaccggc cgttgataac cgatgttctt cactcacttg ttccactggt tccggtagag 1140ctaggaggga ctctccggtt aacaagatga ttcacag 117712154DNAARABIDOPSIS THALIANA 12tcgcaagaac caatctccaa aatccatcaa caactcagat tctgagagtg ggaattcatt 60ttccttgtta atgagacgac ttggctcgat taaaactcag agaagaactt ctatccaaaa 120gggttacgtg caatttttcg atatcaagac cctc 15413288DNAARABIDOPSIS THALIANA 13tctgtgtcag gaatccaaat gggaagtggt gaagaagata gatttgatgc tcataagaaa 60cttctgattg gtctcataat cagtttctct tctcttggcc ttataatctt gttctgtttt 120ggcttttggg tttatcgcaa gaaccaatct ccaaaatcca tcaacaactc agattctgag 180agtgggaatt cattttcctt gttaatgaga cgacttggct cgattaaaac tcagagaaga 240acttctatcc aaaagggtta cgtgcaattt ttcgatatca agaccctc 288141510DNAARABIDOPSIS THALIANA 14tgttaaaagc gatttataat ttacaccgtt ttggtgtata tttctatcta tccttttaca 60agacctatat atgttatgtt atggtggtgt actattttaa gtgagcgaca tagtattttc 120ttcatatagc taattaatca acaacaattt cccaacttac aactatttgc gtactttaaa 180cttatattga aagagaacta caaaattatt tttttgtaca agagaattat ggtcttcgga 240tcaataattt ctctagatat aatatgtaaa gccaacccta taatttgtaa aatccatgat 300ttgatataat tttcttttaa aattgtgaat tggcagacaa aaacaacatt acattttgat 360ttaaattcat aactttgact tgctaaggaa acaccatgat tcattttttg tcatttgtta 420catcatcact agaaatattt gatctaactt tattatgata atagactaca tactacatat 480gcagttacga ttttaaatac tacatattta agcgtgttta aactgtaacc atatcatata 540aaatgacata tctaaaagtg attttcaata ttttgatatg atatgtgttg tagcacggat 600aatgatctaa tttttaagta ataagcttgt tcattacaaa agagaagaaa gtagtattgg 660gccatgatta tgtaaggaca aaataggaag atgtggaaga agccattcga gggttttatt 720acaaaaacag agtatataat tggtcataat gttttattca cttaatttaa cattattgca 780ttatattttc atgaacacat atttctttaa ctaaaaatat acacatattt cttattgtag 840atgaagtgaa aagaacaata tttgggttca catctatggg tgaatccttt taatcacccc 900ctaaaataaa aaaggtgcca tatttctatt tttagagaaa gatatagagc accattggag 960tggttttgct ccaaatatag agtttagaga aatatataat acaccattgg agatgctcta 1020aaatgaattt atttatttat ttagatggaa gattctaatt ggttagaaaa agaggaagtg 1080aataatagga ttcacctata agagtgaacc caagtatttt taagagataa tgtgtaaagt 1140aaatagatgg tcattgtgtg aattatgaat agaaccatgg ttttccattt ttaattgctt 1200aacatagggt aatcaacaat ggggtttaat atgtcaatag acaatagtaa agaaagtatt 1260tgatctatcc caaatctttc ttcgttcgtt agttcatcac tttctttctt tttggttata 1320ttaatggtag agaactaaaa attcaacttt ttattcaaaa gctccctttc tctttccctc 1380ctttatttgc cataaaagtg atttcaagaa gacagcgaga gagaaagtga tagttcgttc 1440actcttcgct ttctcaagaa tttcaaaaca ccaaaaaagt ctttagattg aatttcatca 1500aaaacttttc 151015157DNAARABIDOPSIS THALIANA 15agacaagaaa aaaggaaaca aaattttatg aaagagatct ccattagaga aagagagagc 60gagagagaga ttaatcttgg aagagcaatc tcacattctc acactgctct tagaaaatct 120ctctttcacc attaaaaatc ccaaagagtc tggagaa 157161257DNAARABIDOPSIS THALIANA 16atgggaaaga ttcttcatct tcttcttctt cttcttaagg tctctgttct tgaattcatc 60attagtgttt ctgcttttac ttcacctgct tcacagcctt ctctttctcc tgtttacact 120tccatggctt ccttttctcc agggatccac atgggcaaag gccaagaaca caagttagat 180gcacacaaga aacttctaat cgctctcata atcacctcat cttctctagg actaatactt 240gtatcttgtt tatgcttttg ggtttattgg tctaagaaat ctcccaaaaa caccaagaac 300tcaggtgaga gtaggatttc attatccaag aagggctttg tgcagtcctt cgattacaag 360acactagaga aagcaacagg cggtttcaaa gacggtaatc ttataggacg aggcgggttc 420ggagatgttt acaaggcctg tttaggcaac aacactctag cagcagtcaa aaagatcgaa 480aacgttagtc aagaagcaaa acgagaattt cagaatgaag ttgatttgtt gagcaagatt 540caccacccga acatcatctc attgtttgga tatggaaatg aactcagttc gagttttatc 600gtctacgagc tgatggaaag cggatcattg gatacacagt tacacggacc ttctcgggga 660tcggctttaa catggcacat gcggatgaag attgctcttg atacagcaag agctgttgag 720tatctccacg agcgttgtcg tcctccggtt atccacagag atcttaaatc gtcaaatatt 780ctccttgatt cttccttcaa cgccaagatt tcggattttg gtcttgcggt aatggtgggg 840gctcacggca aaaacaacat taaactatca ggaacacttg gttatgttgc tccagaatat 900ctcctagatg gaaaattgac ggataagagt gatgtttatg cgtttggtgt ggttttactt 960gaactcttgt taggaagacg gccggttgag aaattgagtt cggttcagtg tcaatctctt 1020gtcacttggg caatgcccca acttacggat agatcaaagc ttccgaaaat cgtggatccg 1080gttatcaaag atacaatgga tcataagcac ttataccagg tggcagccgt ggcagtgctt 1140tgtgtacaac cagaaccgag ttatcgaccg ttgataaccg atgttcttca ctcactagtt 1200ccattggttc cggtagagct aggagggact ctccggttaa taccatcatc gtcttga 125717418PRTARABIDOPSIS THALIANA 17Met Gly Lys Ile Leu His Leu Leu Leu Leu Leu Leu Lys Val Ser Val1 5 10 15Leu Glu Phe Ile Ile Ser Val Ser Ala Phe Thr Ser Pro Ala Ser Gln 20 25 30Pro Ser Leu Ser Pro Val Tyr Thr Ser Met Ala Ser Phe Ser Pro Gly 35 40 45Ile His Met Gly Lys Gly Gln Glu His Lys Leu Asp Ala His Lys Lys 50 55 60Leu Leu Ile Ala Leu Ile Ile Thr Ser Ser Ser Leu Gly Leu Ile Leu65 70 75 80Val Ser Cys Leu Cys Phe Trp Val Tyr Trp Ser Lys Lys Ser Pro Lys 85 90 95Asn Thr Lys Asn Ser Gly Glu Ser Arg Ile Ser Leu Ser Lys Lys Gly 100 105 110Phe Val Gln Ser Phe Asp Tyr Lys Thr Leu Glu Lys Ala Thr Gly Gly 115 120 125Phe Lys Asp Gly Asn Leu Ile Gly Arg Gly Gly Phe Gly Asp Val Tyr 130 135 140Lys Ala Cys Leu Gly Asn Asn Thr Leu Ala Ala Val Lys Lys Ile Glu145 150 155 160Asn Val Ser Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val Asp Leu 165 170 175Leu Ser Lys Ile His His Pro Asn Ile Ile Ser Leu Phe Gly Tyr Gly 180 185 190Asn Glu Leu Ser Ser Ser Phe Ile Val Tyr Glu Leu Met Glu Ser Gly 195 200 205Ser Leu Asp Thr Gln Leu His Gly Pro Ser Arg Gly Ser Ala Leu Thr 210 215 220Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Ala Val Glu225 230 235 240Tyr Leu His Glu Arg Cys Arg Pro Pro Val Ile His Arg Asp Leu Lys 245 250 255Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile Ser Asp 260 265 270Phe Gly Leu Ala Val Met Val Gly Ala His Gly Lys Asn Asn Ile Lys 275 280 285Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly 290 295 300Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu305 310 315 320Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Ser Ser Val Gln 325 330 335Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser 340 345 350Lys Leu Pro Lys Ile Val Asp Pro Val Ile Lys Asp Thr Met Asp His 355 360 365Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro 370 375 380Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu Val385 390 395 400Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Ile Pro Ser 405 410 415Ser Ser181302DNAARABIDOPSIS THALIANA 18atgaagcaaa ttgttataac agctcttgtt ttactacaag cttatgttct tcatcaatcc 60acatgtgtta tgtcccttac tacacaagaa tctccttctc ctcaaccttc tgctttcact 120cccgccttat ctcctgatta tcaacagaga gagaaggaat tgcataaaca agagagtaac 180aacatgagac tggttatttc actagcagct acattttcct tagttggtat aatcttactt 240tgctctctgc tttattggtt ttgccatagg agaagaaacc tcaagagctc aggttgtggg 300tgtagtggaa tcacattctt gaatcggttt agtcgctcaa aaacattaga caagagaact 360acaaagcagg gaacagtgtc attgatcgat tacaatatac tagaagaagg aactagtggt 420ttcaaggaga gtaacatttt gggtcaaggt ggatttggat gtgtatattc tgccacatta 480gagaacaaca tttcagctgc ggttaagaag ctagactgtg ccaatgaaga tgcagcaaag 540gaatttaaga gtgaggttga gatattgagt aagctccagc acccgaatat aatatccctt 600ttgggttata gcacgaatga tactgcgaga ttcattgtct atgagctgat gccaaacgtt 660tctctggaat ctcatttaca cggatcttct cagggttcgg cgatcacatg gcctatgagg 720atgaagattg ctcttgatgt aacaagggga ttagaatatt tgcatgaaca ttgtcatcca 780gcaatcattc acagggactt gaaatcatcc aacatcttat tagatagcaa tttcaatgct 840aagatttcag attttggtct agctgttgtt gatgggccaa agaacaagaa ccataaactt 900tccgggacag ttggctacgt tgcaccagag tatcttctca acggccaatt gacagaaaag 960agcgacgtgt atgcttttgg agtagtgtta ttagagcttt tactcgggaa aaaacctgtg 1020gagaaactag ctcccggtga atgccaatcc atcatcactt gggcaatgcc ttatctcact 1080gatagaacca agttaccaag cgtcatagat cctgcgatta aagatacgat ggacttgaaa 1140cacctttacc aggtagcggc agtggcgatt ttgtgcgtgc agccagaacc gagttataga 1200ccgttgatta cagacgtctt gcattctctt atacctttgg ttccaatgga acttggtgga 1260accttaaaaa ccatcaaatg tgcttcaatg gatcactgtt aa 130219433PRTARABIDOPSIS THALIANA 19Met Lys Gln Ile Val Ile Thr Ala Leu Val Leu Leu Gln Ala Tyr Val1 5 10 15Leu His Gln Ser Thr Cys Val Met Ser Leu Thr Thr Gln Glu Ser Pro 20 25 30Ser Pro Gln Pro Ser Ala Phe Thr Pro Ala Leu Ser Pro Asp Tyr Gln 35 40 45Gln Arg Glu Lys Glu Leu His Lys Gln Glu Ser Asn Asn Met Arg Leu 50 55 60Val Ile Ser Leu Ala Ala Thr Phe Ser Leu Val Gly Ile Ile Leu Leu65 70 75 80Cys Ser Leu Leu Tyr Trp Phe Cys His Arg Arg Arg Asn Leu Lys Ser 85 90 95Ser Gly Cys Gly Cys Ser Gly Ile Thr Phe Leu Asn Arg Phe Ser Arg 100 105 110Ser Lys Thr Leu Asp Lys Arg Thr Thr Lys Gln Gly Thr Val Ser Leu 115 120 125Ile Asp Tyr Asn Ile Leu Glu Glu Gly Thr Ser Gly Phe Lys Glu Ser 130 135 140Asn Ile Leu Gly Gln Gly Gly Phe Gly Cys Val Tyr Ser Ala Thr Leu145 150 155 160Glu Asn Asn Ile Ser Ala Ala Val Lys Lys Leu Asp Cys Ala Asn Glu 165 170 175Asp Ala Ala Lys Glu Phe Lys Ser Glu Val Glu Ile Leu Ser Lys Leu 180 185 190Gln His Pro Asn Ile Ile Ser Leu Leu Gly Tyr Ser Thr Asn Asp Thr 195 200 205Ala Arg Phe Ile Val Tyr Glu Leu Met Pro Asn Val Ser Leu Glu Ser 210 215 220His Leu His Gly Ser Ser Gln Gly Ser Ala Ile Thr Trp Pro Met Arg225 230 235 240Met Lys Ile Ala Leu Asp Val Thr Arg Gly Leu Glu Tyr Leu His Glu 245 250 255His Cys His Pro Ala Ile Ile His Arg Asp Leu Lys Ser Ser Asn Ile 260 265 270Leu Leu Asp Ser Asn Phe Asn Ala Lys Ile Ser Asp Phe Gly Leu Ala 275 280 285Val Val Asp Gly Pro Lys Asn Lys Asn His Lys Leu Ser Gly Thr Val 290 295 300Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asn Gly Gln Leu Thr Glu Lys305 310 315 320Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Leu Gly 325 330 335Lys Lys Pro Val Glu Lys Leu Ala Pro Gly Glu Cys Gln Ser Ile Ile 340 345 350Thr Trp Ala Met Pro Tyr Leu Thr Asp Arg Thr Lys Leu Pro Ser Val 355 360 365Ile Asp Pro Ala Ile Lys Asp Thr Met Asp Leu Lys His Leu Tyr Gln 370 375 380Val Ala Ala Val Ala Ile Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg385 390 395 400Pro Leu Ile Thr Asp Val Leu His Ser Leu Ile Pro Leu Val Pro Met 405 410 415Glu Leu Gly Gly Thr Leu Lys Thr Ile Lys Cys Ala Ser Met Asp His 420 425 430Cys202016DNAARABIDOPSIS THALIANA 20atgaagacta tgtccaaatc gtctttgcgt ttgcattttc tctcgctact cttactttgt 60tgtgtctccc cttcaagctt tgtcattata agattcatta cacataatca ttttgatggt 120ctagtacgtt gtcatcccca caagtttcaa gcccttacgc agttcaagaa cgagtttgat 180acccgccgtt gcaaccacag taactacttt aatggaatct ggtgtgataa ctccaaggtg 240cggtcacaaa gctacgacta cgggactgtc tcagtggaac tctcaaatca aacagtagcc 300tcttccagtt tcatcatctt cgctaccttg atctctctca caacaacttc acctcctctt 360ccctcccttc cgagtttgtt tcccactttg cggaatctaa ccaagctcac agttttagac 420ctttctcata atcacttctc cggaactttg aagcccaaca atagcctctt tgagttacac 480caccttcgtt accttaatct cgaggtcaac

aacttcagtt cctcactccc ttccgagttt 540ggctatctca acaatttaca gcactgtggc ctcaaagagt tcccaaacat attcaagacc 600cttaaaaaaa tggaggctat agacgtatcc aacaatagaa tcaacgggaa aatccctgag 660tggttatgga gccttcctct tcttcattta gtgaatattt taaataattc ttttgacggt 720ttcgaaggat caacggaagt tttagtaaat tcatcggttc ggatattact tttggagtca 780aacaactttg aaggagcact tcctagtcta ccacactcta tcaacgcctt ctccgcgggt 840cataacaatt tcactggaga gatacctctt tcaatctgca ccagaacctc acttggtgtc 900cttgatctaa actacaacaa cctcattggt ccggtttctc aatgtttgag taatgtcacg 960tttgtaaatc tccggaaaaa caatttggaa ggaactattc ctgagacttt cattgtcggt 1020tcctcgataa ggacacttga tgttggatac aatcgactaa cgggaaagct tccaaggtct 1080cttttgaact gctcatctct agagtttcta agcgttgaca acaacagaat caaagacaca 1140tttcctttct ggctcaaggc tttaccaaag ttacaagtcc ttaccctaag ttcaaacaag 1200ttttatggtc ctatatctcc tcctcatcaa ggtcctctcg ggtttccaga gctgagaata 1260cttgagatat ctgataataa gtttactgga agcttgtcgt caagatactt tgagaattgg 1320aaagcatcgt ccgccatgat gaatgaatat gtgggtttat atatggttta cgagaagaat 1380ccttatggtg tagttgtcta tacctttttg gatcgtatag atttgaaata caaaggtcta 1440aacatggagc aagcgagggt tctcacttcc tacagcgcca ttgatttttc tagaaatcta 1500cttgaaggaa atattcctga atccattgga cttttaaagg cattgattgc actaaactta 1560tcgaacaacg cttttacagg ccatattcct cagtctttgg caaatcttaa ggagctccag 1620tcactagaca tgtctaggaa ccaactctca gggactattc ctaatggact caagcaactc 1680tcgtttttgg cttacataag tgtgtctcat aaccaactca agggtgaaat accacaagga 1740acacaaatta ctgggcaatt gaaatcttcc tttgaaggga atgtaggact ttgtggtctt 1800cctctcgagg aaaggtgctt cgacaatagt gcatctccaa cgcagcacca caagcaagac 1860gaagaagaag aagaagaaca agtgttacac tggaaagcgg tggcaatggg gtatggacct 1920ggattgttgg ttggatttgc aattgcatat gtcattgctt catacaagcc ggagtggcta 1980accaagataa ttggtccgaa taagcgcaga aactag 201621671PRTARABIDOPSIS THALIANA 21Met Lys Thr Met Ser Lys Ser Ser Leu Arg Leu His Phe Leu Ser Leu1 5 10 15Leu Leu Leu Cys Cys Val Ser Pro Ser Ser Phe Val Ile Ile Arg Phe 20 25 30Ile Thr His Asn His Phe Asp Gly Leu Val Arg Cys His Pro His Lys 35 40 45Phe Gln Ala Leu Thr Gln Phe Lys Asn Glu Phe Asp Thr Arg Arg Cys 50 55 60Asn His Ser Asn Tyr Phe Asn Gly Ile Trp Cys Asp Asn Ser Lys Val65 70 75 80Arg Ser Gln Ser Tyr Asp Tyr Gly Thr Val Ser Val Glu Leu Ser Asn 85 90 95Gln Thr Val Ala Ser Ser Ser Phe Ile Ile Phe Ala Thr Leu Ile Ser 100 105 110Leu Thr Thr Thr Ser Pro Pro Leu Pro Ser Leu Pro Ser Leu Phe Pro 115 120 125Thr Leu Arg Asn Leu Thr Lys Leu Thr Val Leu Asp Leu Ser His Asn 130 135 140His Phe Ser Gly Thr Leu Lys Pro Asn Asn Ser Leu Phe Glu Leu His145 150 155 160His Leu Arg Tyr Leu Asn Leu Glu Val Asn Asn Phe Ser Ser Ser Leu 165 170 175Pro Ser Glu Phe Gly Tyr Leu Asn Asn Leu Gln His Cys Gly Leu Lys 180 185 190Glu Phe Pro Asn Ile Phe Lys Thr Leu Lys Lys Met Glu Ala Ile Asp 195 200 205Val Ser Asn Asn Arg Ile Asn Gly Lys Ile Pro Glu Trp Leu Trp Ser 210 215 220Leu Pro Leu Leu His Leu Val Asn Ile Leu Asn Asn Ser Phe Asp Gly225 230 235 240Phe Glu Gly Ser Thr Glu Val Leu Val Asn Ser Ser Val Arg Ile Leu 245 250 255Leu Leu Glu Ser Asn Asn Phe Glu Gly Ala Leu Pro Ser Leu Pro His 260 265 270Ser Ile Asn Ala Phe Ser Ala Gly His Asn Asn Phe Thr Gly Glu Ile 275 280 285Pro Leu Ser Ile Cys Thr Arg Thr Ser Leu Gly Val Leu Asp Leu Asn 290 295 300Tyr Asn Asn Leu Ile Gly Pro Val Ser Gln Cys Leu Ser Asn Val Thr305 310 315 320Phe Val Asn Leu Arg Lys Asn Asn Leu Glu Gly Thr Ile Pro Glu Thr 325 330 335Phe Ile Val Gly Ser Ser Ile Arg Thr Leu Asp Val Gly Tyr Asn Arg 340 345 350Leu Thr Gly Lys Leu Pro Arg Ser Leu Leu Asn Cys Ser Ser Leu Glu 355 360 365Phe Leu Ser Val Asp Asn Asn Arg Ile Lys Asp Thr Phe Pro Phe Trp 370 375 380Leu Lys Ala Leu Pro Lys Leu Gln Val Leu Thr Leu Ser Ser Asn Lys385 390 395 400Phe Tyr Gly Pro Ile Ser Pro Pro His Gln Gly Pro Leu Gly Phe Pro 405 410 415Glu Leu Arg Ile Leu Glu Ile Ser Asp Asn Lys Phe Thr Gly Ser Leu 420 425 430Ser Ser Arg Tyr Phe Glu Asn Trp Lys Ala Ser Ser Ala Met Met Asn 435 440 445Glu Tyr Val Gly Leu Tyr Met Val Tyr Glu Lys Asn Pro Tyr Gly Val 450 455 460Val Val Tyr Thr Phe Leu Asp Arg Ile Asp Leu Lys Tyr Lys Gly Leu465 470 475 480Asn Met Glu Gln Ala Arg Val Leu Thr Ser Tyr Ser Ala Ile Asp Phe 485 490 495Ser Arg Asn Leu Leu Glu Gly Asn Ile Pro Glu Ser Ile Gly Leu Leu 500 505 510Lys Ala Leu Ile Ala Leu Asn Leu Ser Asn Asn Ala Phe Thr Gly His 515 520 525Ile Pro Gln Ser Leu Ala Asn Leu Lys Glu Leu Gln Ser Leu Asp Met 530 535 540Ser Arg Asn Gln Leu Ser Gly Thr Ile Pro Asn Gly Leu Lys Gln Leu545 550 555 560Ser Phe Leu Ala Tyr Ile Ser Val Ser His Asn Gln Leu Lys Gly Glu 565 570 575Ile Pro Gln Gly Thr Gln Ile Thr Gly Gln Leu Lys Ser Ser Phe Glu 580 585 590Gly Asn Val Gly Leu Cys Gly Leu Pro Leu Glu Glu Arg Cys Phe Asp 595 600 605Asn Ser Ala Ser Pro Thr Gln His His Lys Gln Asp Glu Glu Glu Glu 610 615 620Glu Glu Gln Val Leu His Trp Lys Ala Val Ala Met Gly Tyr Gly Pro625 630 635 640Gly Leu Leu Val Gly Phe Ala Ile Ala Tyr Val Ile Ala Ser Tyr Lys 645 650 655Pro Glu Trp Leu Thr Lys Ile Ile Gly Pro Asn Lys Arg Arg Asn 660 665 670221662DNAARABIDOPSIS THALIANA 22atgacttcct ctcgccgtct tcttcttcct ctcggagcat cgctcactag aggaagattt 60tcttccgatc aaatccgaaa tggatttcta agaaacttcc gtggattcgc caccgtaact 120tcgtcggaac cggccttagc caatctggaa gcgaaatatg ccgtagcgtt gccagaatgt 180tcaacagtag aggacgagat cacgaagatc cgtcatgaat tcgagttagc gaaacagagg 240tttcttaata tccctgaagc tattaatagt atgccgaaga tgaatcctca agggatatat 300gtgaataaga atctgagatt ggataatata caagtttatg gatttgatta tgattacact 360ttggcacatt actcttctca cttacagagt ttgatctatg atcttgccaa gaaacatatg 420gttaatgagt ttagatatcc tgatgtttgc actcagtttg agtatgatcc tacttttcca 480atccgtgggt tgtactatga taaactaaaa ggatgcctca tgaaattgga tttcttcggt 540tcaatcgagc cagatgggtg ttattttggt cgtcgtaagc ttagtaggaa ggaaatagaa 600agcatgtatg gaacgcggca cataggtcgt gatcaagcga gaggtttggt gggattgatg 660gatttcttct gttttagcga ggcgtgtctt atagcagaca tggtgcaata ttttgttgac 720gccaaacttg agtttgatgc ctctaacatc tacaatgatg tcaatcgtgc tattcaacat 780gtccatagaa gtggattggt tcatagagga attcttgctg atcccaacag atatttgcta 840aaaaatggtc agcttctacg tttcctgaga atgctaaaag ataaaggaaa gaagcttttt 900ttgctgacca actctccgta taattttgtt gatggcggaa tgcgctttct aatggaggaa 960tcttttggct tcggagattc ctggcgagaa ctctttgatg ttgtgattgc taaagcaaat 1020aaaccagaat tttacacatc tgagcaccct ttccgttgtt atgattcgga gagggataat 1080ttggcattta caaaagtgga tgcatttgac ccaaagaaag tttattatca tggttgtctt 1140aaatccttcc ttgaaatcac aaagtggcat ggccctgagg tgatttattt cggagatcac 1200ttatttagtg atctaagagg gccttcaaaa gctggttggc gaactgctgc cataattcat 1260gagctcgagc gagagataca gatacaaaat gatgatagct accggtttga gcaggccaag 1320ttccatatta tccaagagtt actcggtaga tttcacgcga ctgtatcaaa caatcagaga 1380agtgaagcat gccaatcact tttggatgag ctgaacaatg cgaggcagag agcaagagac 1440acgatgaaac aaatgttcaa cagatcgttt ggagctacat ttgtcacaga cactggtcaa 1500gaatcagcat tctcttatca catccaccaa tacgcagacg tttataccag taaacctgag 1560aactttctgt tataccgacc tgaagcctgg cttcacgttc cttacgatat caagatcatg 1620ccacatcatg tcaaggttgc ttcaaccctt ttcaaaacct ga 166223553PRTARABIDOPSIS THALIANA 23Met Thr Ser Ser Arg Arg Leu Leu Leu Pro Leu Gly Ala Ser Leu Thr1 5 10 15Arg Gly Arg Phe Ser Ser Asp Gln Ile Arg Asn Gly Phe Leu Arg Asn 20 25 30Phe Arg Gly Phe Ala Thr Val Thr Ser Ser Glu Pro Ala Leu Ala Asn 35 40 45Leu Glu Ala Lys Tyr Ala Val Ala Leu Pro Glu Cys Ser Thr Val Glu 50 55 60Asp Glu Ile Thr Lys Ile Arg His Glu Phe Glu Leu Ala Lys Gln Arg65 70 75 80Phe Leu Asn Ile Pro Glu Ala Ile Asn Ser Met Pro Lys Met Asn Pro 85 90 95Gln Gly Ile Tyr Val Asn Lys Asn Leu Arg Leu Asp Asn Ile Gln Val 100 105 110Tyr Gly Phe Asp Tyr Asp Tyr Thr Leu Ala His Tyr Ser Ser His Leu 115 120 125Gln Ser Leu Ile Tyr Asp Leu Ala Lys Lys His Met Val Asn Glu Phe 130 135 140Arg Tyr Pro Asp Val Cys Thr Gln Phe Glu Tyr Asp Pro Thr Phe Pro145 150 155 160Ile Arg Gly Leu Tyr Tyr Asp Lys Leu Lys Gly Cys Leu Met Lys Leu 165 170 175Asp Phe Phe Gly Ser Ile Glu Pro Asp Gly Cys Tyr Phe Gly Arg Arg 180 185 190Lys Leu Ser Arg Lys Glu Ile Glu Ser Met Tyr Gly Thr Arg His Ile 195 200 205Gly Arg Asp Gln Ala Arg Gly Leu Val Gly Leu Met Asp Phe Phe Cys 210 215 220Phe Ser Glu Ala Cys Leu Ile Ala Asp Met Val Gln Tyr Phe Val Asp225 230 235 240Ala Lys Leu Glu Phe Asp Ala Ser Asn Ile Tyr Asn Asp Val Asn Arg 245 250 255Ala Ile Gln His Val His Arg Ser Gly Leu Val His Arg Gly Ile Leu 260 265 270Ala Asp Pro Asn Arg Tyr Leu Leu Lys Asn Gly Gln Leu Leu Arg Phe 275 280 285Leu Arg Met Leu Lys Asp Lys Gly Lys Lys Leu Phe Leu Leu Thr Asn 290 295 300Ser Pro Tyr Asn Phe Val Asp Gly Gly Met Arg Phe Leu Met Glu Glu305 310 315 320Ser Phe Gly Phe Gly Asp Ser Trp Arg Glu Leu Phe Asp Val Val Ile 325 330 335Ala Lys Ala Asn Lys Pro Glu Phe Tyr Thr Ser Glu His Pro Phe Arg 340 345 350Cys Tyr Asp Ser Glu Arg Asp Asn Leu Ala Phe Thr Lys Val Asp Ala 355 360 365Phe Asp Pro Lys Lys Val Tyr Tyr His Gly Cys Leu Lys Ser Phe Leu 370 375 380Glu Ile Thr Lys Trp His Gly Pro Glu Val Ile Tyr Phe Gly Asp His385 390 395 400Leu Phe Ser Asp Leu Arg Gly Pro Ser Lys Ala Gly Trp Arg Thr Ala 405 410 415Ala Ile Ile His Glu Leu Glu Arg Glu Ile Gln Ile Gln Asn Asp Asp 420 425 430Ser Tyr Arg Phe Glu Gln Ala Lys Phe His Ile Ile Gln Glu Leu Leu 435 440 445Gly Arg Phe His Ala Thr Val Ser Asn Asn Gln Arg Ser Glu Ala Cys 450 455 460Gln Ser Leu Leu Asp Glu Leu Asn Asn Ala Arg Gln Arg Ala Arg Asp465 470 475 480Thr Met Lys Gln Met Phe Asn Arg Ser Phe Gly Ala Thr Phe Val Thr 485 490 495Asp Thr Gly Gln Glu Ser Ala Phe Ser Tyr His Ile His Gln Tyr Ala 500 505 510Asp Val Tyr Thr Ser Lys Pro Glu Asn Phe Leu Leu Tyr Arg Pro Glu 515 520 525Ala Trp Leu His Val Pro Tyr Asp Ile Lys Ile Met Pro His His Val 530 535 540Lys Val Ala Ser Thr Leu Phe Lys Thr545 550241386DNABRACHYPODIUM DISTACHYON 24atggagattc cggcggcgcc gccgcctcca ttgccggtgc tgtgctcgta cgtcgtcttc 60ttgctgctgc tgtcttcgtg ctcactggcc agagggagga tcgcggtttc ttccccgggc 120ccgtcgcctg tggccgccgc cgttacagcc aatgagaccg cttcatcctc ttcttctccg 180gtgtttccgg ccgctcctcc cgtcgtgatc acagtggtga ggcaccacca ttaccaccgg 240gagctggtca tctccgctgt cctcgcctgc gtcgccaccg ccatgatcct cctctccaca 300ctctacgcct ggacgatgtg gcggcggtct cgccggaccc cccacggcgg caagggccgc 360ggccggagat cagggatcac actggtgcca atcctgagca agttcaattc agtgaagatg 420agcaggaagg ggggccttgt gacgatgatc gagtacccgt cgctggaggc ggcgacaggc 480aagttcggcg agagcaatgt gctcggtgtc ggcggcttcg gttgcgttta taaggcggcg 540tttgatggcg gtgccaccgc cgccgtgaag aggcttgaag gcggcgggcc ggattgcgag 600aaggaattcg agaatgagct ggatttgctt ggcaggatca ggcacccaaa catagtgtct 660ctcctgggct tctgtgtcca tggtggcaat cactacattg tttatgagct catggagaag 720ggatcattgg agacacagct gcatgggtct tcacatggat ctgctctgag ctggcacgtt 780cggatgaaga tcgcgctcga tacggcgagg ggattagagt atcttcatga gcactgcaat 840ccacctgtga tccataggga tctgaaacct tctaatatac ttttagattc agacttcaat 900gctaagattg cagattttgg ccttgcggtc accggtggga atctcaacaa agggaacctg 960aagctttccg ggaccttggg ttatgtagcc cctgagtact tattagatgg gaagttgact 1020gagaagagcg atgtatacgc atttggagta gtgcttctag agctcctgat gggaaggaag 1080cctgttgaga aaatgtcacc atctcagtgc caatcaattg tgtcatgggc tatgcctcag 1140ctgaccgaca gatcgaagct ccccaacata attgacctgg tgatcaagga caccatggac 1200ccaaaacact tgtaccaagt tgcagcagtg gctgttctat gtgtgcagcc cgaaccgagc 1260tacagaccac tgataacaga tgttctccac tctcttgttc ctctagtgcc tgcggagctc 1320ggaggaacac tcagggttgc agagccacct tcaccttctc cagaccaaag acattatcct 1380tgttga 1386251302DNABRASSICA NAPUS 25atgaagaaac tggttcatct tcagtttttg tttcttgtca agatctttgc tactcaattc 60ctcactcctt cttcatcatc ttttgctgct tcaaatcctt ctatagctcc tgtttacacc 120tccatgacta ctttctctcc aggaattcaa atgggaagtg gtgaagaaca cagattagat 180gcacataaga aactcctgat tggtcttata atcagttcct cttctcttgg tatcataatc 240ttgatttgct ttggcttctg gatgtactgt cgcaagaaag ctcccaaacc catcaagatt 300ccggatgccg agagtgggac ttcatcattt tcaatgtttg tgaggcggct aagctcaatt 360aaaactcaca gaacatctag caatcagggt tatgtgcagc gtttcgattc caagacgcta 420gagaaagcga caggcggttt caaagacagt aatgtaatcg gacagggcgg tttcggatgc 480gtttacaagg cttctttgga cagcaacact aaagcagcgg ttaaaaagat cgaaaacgtt 540acccaagaag caaaacgaga atttcagaat gaagttgagc tgttgagcaa gatccagcac 600tccaatatta tatcattgtt gggctctgca agtgaaatca actcgagttt cgtcgtttat 660gagttgatgg agaaaggatc cttagatgat cagttacatg gaccttcgtg tggatccgct 720ctaacatggc atatgcgtat gaagattgct ctagatacag ctagaggact agagtatctc 780catgaacatt gtcgtccacc agttatccac agggacctga aatcgtctaa tattcttctt 840gattcttcct tcaatgccaa gatttcagat tttggtctgg ctgtatcggt tggagtgcat 900gggagtaaca acattaaact ctctgggaca cttggttatg ttgccccgga atatctccta 960gacggaaagt tgacggataa gagtgatgtc tatgcatttg gggtggttct tcttgaactt 1020ttgttgggta ggcggccggt tgagaaattg agtccatctc agtgtcaatc tcttgtgact 1080tgggcaatgc cacaacttac cgatagatcg aaactcccaa acatcgtgga tccggttata 1140aaagatacaa tggatcttaa gcacttatac caagtagcag ccatggctgt gctgtgcgta 1200cagccagaac cgagttaccg gccgctgata accgatgttc ttcattcact tgttccattg 1260gttccggtag agctaggagg gactctccgg ttaacccgat ga 130226433PRTBRASSICA NAPUS 26Met Lys Lys Leu Val His Leu Gln Phe Leu Phe Leu Val Lys Ile Phe1 5 10 15Ala Thr Gln Phe Leu Thr Pro Ser Ser Ser Ser Phe Ala Ala Ser Asn 20 25 30Pro Ser Ile Ala Pro Val Tyr Thr Ser Met Thr Thr Phe Ser Pro Gly 35 40 45Ile Gln Met Gly Ser Gly Glu Glu His Arg Leu Asp Ala His Lys Lys 50 55 60Leu Leu Ile Gly Leu Ile Ile Ser Ser Ser Ser Leu Gly Ile Ile Ile65 70 75 80Leu Ile Cys Phe Gly Phe Trp Met Tyr Cys Arg Lys Lys Ala Pro Lys 85 90 95Pro Ile Lys Ile Pro Asp Ala Glu Ser Gly Thr Ser Ser Phe Ser Met 100 105 110Phe Val Arg Arg Leu Ser Ser Ile Lys Thr His Arg Thr Ser Ser Asn 115 120 125Gln Gly Tyr Val Gln Arg Phe Asp Ser Lys Thr Leu Glu Lys Ala Thr 130 135 140Gly Gly Phe Lys Asp Ser Asn Val Ile Gly Gln Gly Gly Phe Gly Cys145 150 155 160Val Tyr Lys Ala Ser Leu Asp Ser Asn Thr Lys Ala Ala Val Lys Lys 165 170 175Ile Glu Asn Val Thr Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val 180 185 190Glu Leu Leu Ser Lys Ile Gln His Ser Asn Ile Ile Ser Leu Leu Gly 195 200 205Ser Ala Ser Glu Ile Asn Ser Ser Phe Val Val Tyr Glu Leu Met Glu 210 215 220Lys Gly Ser Leu Asp Asp Gln Leu His Gly Pro Ser Cys Gly Ser Ala225 230 235 240Leu Thr Trp His Met Arg Met

Lys Ile Ala Leu Asp Thr Ala Arg Gly 245 250 255Leu Glu Tyr Leu His Glu His Cys Arg Pro Pro Val Ile His Arg Asp 260 265 270Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile 275 280 285Ser Asp Phe Gly Leu Ala Val Ser Val Gly Val His Gly Ser Asn Asn 290 295 300Ile Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu305 310 315 320Asp Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val 325 330 335Leu Leu Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Ser Pro 340 345 350Ser Gln Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp 355 360 365Arg Ser Lys Leu Pro Asn Ile Val Asp Pro Val Ile Lys Asp Thr Met 370 375 380Asp Leu Lys His Leu Tyr Gln Val Ala Ala Met Ala Val Leu Cys Val385 390 395 400Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser 405 410 415Leu Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Thr 420 425 430Arg27657DNACICHORIUM ENDIVIA 27atttttggtg ttgaaatgat gcacaacgga tctttggaat cccaattgca tggtccgtct 60catggaactg gcttaagctg gcagcatcga atgaaaattg cacttgatat tgcacgagga 120ctagagtatc ttcacgagcg ctgtaccccg cctgtgattc atagagatct gaaatcgtcc 180aacattcttc taggttcgaa ctacaatgct aaactttctg atttcgggct cgcgattact 240ggtgggattc agggcaagaa caacgtaaag ctttcgggaa cattaggtta tgtagctcca 300gaatacctct tagatggtaa acttactgat aaaagtgatg tttatgcgtt tggagttgta 360cttcttgaac ttttgatagg tagaaaacca gtggagaaaa tgtcaccatc tcaatgccaa 420tctatcgtta catgggcaat gcctcaacta accgaccgat caaagcttcc taacatcgtt 480gatcccgtga ttagagatac aatggacttg aagcacttgt atcaagttgc tgcggttgct 540gtgctatgtg tacaaccgga accgagttac aggccattga taacagatgt tttgcattcg 600ttcatcccac ttgtacctgt tgagcttgga gggtcgctaa gagttaccga atcttga 65728218PRTCICHORIUM ENDIVIA 28Ile Phe Gly Val Glu Met Met His Asn Gly Ser Leu Glu Ser Gln Leu1 5 10 15His Gly Pro Ser His Gly Thr Gly Leu Ser Trp Gln His Arg Met Lys 20 25 30Ile Ala Leu Asp Ile Ala Arg Gly Leu Glu Tyr Leu His Glu Arg Cys 35 40 45Thr Pro Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu 50 55 60Gly Ser Asn Tyr Asn Ala Lys Leu Ser Asp Phe Gly Leu Ala Ile Thr65 70 75 80Gly Gly Ile Gln Gly Lys Asn Asn Val Lys Leu Ser Gly Thr Leu Gly 85 90 95Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys Ser 100 105 110Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Ile Gly Arg 115 120 125Lys Pro Val Glu Lys Met Ser Pro Ser Gln Cys Gln Ser Ile Val Thr 130 135 140Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Ile Val145 150 155 160Asp Pro Val Ile Arg Asp Thr Met Asp Leu Lys His Leu Tyr Gln Val 165 170 175Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro 180 185 190Leu Ile Thr Asp Val Leu His Ser Phe Ile Pro Leu Val Pro Val Glu 195 200 205Leu Gly Gly Ser Leu Arg Val Thr Glu Ser 210 21529474DNACITRUS CLEMENTINA 29aattcggcac gagggctgga ttccagtttt aatgcaaagc tttcagattt tggcctttct 60gtgactgctg gaacccagag taggaatgtt aagatctctg gaactctggg ttatgttgcc 120ccggagtacc tattagaagg aaaactaact gataaaagtg atgtatatgc tttcggagtt 180gtattgctgg aacttttgat ggggagaagg cctgtggaaa agatgtcacc aactcaatgt 240caatcaatgg tcacatgggc catgcctcag ctcaccgata gatcaaagct tccaaacatt 300gtggatccag taattagaga cacaatggat ttaaagcact tataccaggt agccgctgtg 360gcagtgctat gtatacaacc tgaaccaagt tataggccat tgataaccga cgttctgcat 420tccctcattc ctcttgtacc taccgacctt ggagggtcac tccgagtgac ctaa 47430157PRTCITRUS CLEMENTINA 30Asn Ser Ala Arg Gly Leu Asp Ser Ser Phe Asn Ala Lys Leu Ser Asp1 5 10 15Phe Gly Leu Ser Val Thr Ala Gly Thr Gln Ser Arg Asn Val Lys Ile 20 25 30Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Glu Gly Lys 35 40 45Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu 50 55 60Leu Leu Met Gly Arg Arg Pro Val Glu Lys Met Ser Pro Thr Gln Cys65 70 75 80Gln Ser Met Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys 85 90 95Leu Pro Asn Ile Val Asp Pro Val Ile Arg Asp Thr Met Asp Leu Lys 100 105 110His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Ile Gln Pro Glu 115 120 125Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu Ile Pro 130 135 140Leu Val Pro Thr Asp Leu Gly Gly Ser Leu Arg Val Thr145 150 15531770DNACITRUS SINENSIS 31ggattgtgtt tgtggcttta tcatttgaag tactccttca aatccagtaa caagaatgca 60aagagcaaag attctgagaa tggagttgtg ttatcatcat ttttgggcaa attcacttct 120gtgaggatgg ttagtaagaa gggatctgct atttcattta ttgagtataa gctgttagag 180aaagccaccg acagttttca tgagagtaat atattgggtg agggtggatt tggatgtgtt 240tacaaggcta aattggatga taacttgcac gtcgctgtca aaaaattaga ttgtgcaaca 300caagatgccg gcagagaatt tgagaatgag gtggatttgc tgagtaatat tcaccaccca 360aatgttgttt gtctgttggg ttatagtgct catgatgaca caaggtttat tgtttatgaa 420ttgatggaaa atcggtccct tgatattcaa ttgcatggtc cttctcatgg atcagcattg 480acttggcata tgcgaatgaa aattgctctt gataccgcta gaggattaga atatttacat 540gagcactgca accctgcagt cattcataga gatctgaaat cctccaatat acttctagat 600tccaagttta atgctaagct ctcagatttt ggtcttgcca taaccgatgg atcccaaaac 660aagaacaatc ttaagctttc gggcactttg ggatatgtgg ctcccgagta tcttttagat 720ggtaaattga cagacaagag tgatgtctat gcttttggag ttgtgcttct 77032257PRTCITRUS SINENSIS 32Gly Leu Cys Leu Trp Leu Tyr His Leu Lys Tyr Ser Phe Lys Ser Ser1 5 10 15Asn Lys Asn Ala Lys Ser Lys Asp Ser Glu Asn Gly Val Val Leu Ser 20 25 30Ser Phe Leu Gly Lys Phe Thr Ser Val Arg Met Val Ser Lys Lys Gly 35 40 45Ser Ala Ile Ser Phe Ile Glu Tyr Lys Leu Leu Glu Lys Ala Thr Asp 50 55 60Ser Phe His Glu Ser Asn Ile Leu Gly Glu Gly Gly Phe Gly Cys Val65 70 75 80Tyr Lys Ala Lys Leu Asp Asp Asn Leu His Val Ala Val Lys Lys Leu 85 90 95Asp Cys Ala Thr Gln Asp Ala Gly Arg Glu Phe Glu Asn Glu Val Asp 100 105 110Leu Leu Ser Asn Ile His His Pro Asn Val Val Cys Leu Leu Gly Tyr 115 120 125Ser Ala His Asp Asp Thr Arg Phe Ile Val Tyr Glu Leu Met Glu Asn 130 135 140Arg Ser Leu Asp Ile Gln Leu His Gly Pro Ser His Gly Ser Ala Leu145 150 155 160Thr Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu 165 170 175Glu Tyr Leu His Glu His Cys Asn Pro Ala Val Ile His Arg Asp Leu 180 185 190Lys Ser Ser Asn Ile Leu Leu Asp Ser Lys Phe Asn Ala Lys Leu Ser 195 200 205Asp Phe Gly Leu Ala Ile Thr Asp Gly Ser Gln Asn Lys Asn Asn Leu 210 215 220Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp225 230 235 240Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu 245 250 255Leu33621DNACOFFEA CANEPHORA 33gcattgacat ggcatcttag gatgaaaatt gcccttgatg tagctagagg attagaattt 60ttgcatgagc actgccaccc agcagtgatc catagagatc tgaaatcatc taatatcctt 120ctggattcaa atctcaatgc taagctatct gattttggtc ttgccattct tgatggggct 180caaaataaga acaacatcaa gctttctgga accttgggct atgtagctcc agagtacctc 240ttagatggta aattgactga caagagtgat gtttatgctt ttggagtggt gcttttggag 300cttctcctga gaagaaagcc tgtggagaag ctggcaccag ctcaatgcca atctatagtc 360acatgggcta tgcctcagct gacagataga tcaaagcttc caaacatcgt ggatcctgtg 420attagaaatg ctatggatat aaagcactta ttccaggttg ctgcagtcgc tgtgctatgc 480gtgcagcctg aaccaagcta tcgaccactg ataacagatg tgttgcattc ccttgttccc 540cttgttccta tggagcttgg cgggacgctc agagttgaac gacctgcttc tgtgacctct 600ctgttgattg attctacctg a 62134206PRTCOFFEA CANEPHORA 34Ala Leu Thr Trp His Leu Arg Met Lys Ile Ala Leu Asp Val Ala Arg1 5 10 15Gly Leu Glu Phe Leu His Glu His Cys His Pro Ala Val Ile His Arg 20 25 30Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asn Leu Asn Ala Lys 35 40 45Leu Ser Asp Phe Gly Leu Ala Ile Leu Asp Gly Ala Gln Asn Lys Asn 50 55 60Asn Ile Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu65 70 75 80Leu Asp Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val 85 90 95Val Leu Leu Glu Leu Leu Leu Arg Arg Lys Pro Val Glu Lys Leu Ala 100 105 110Pro Ala Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu Thr 115 120 125Asp Arg Ser Lys Leu Pro Asn Ile Val Asp Pro Val Ile Arg Asn Ala 130 135 140Met Asp Ile Lys His Leu Phe Gln Val Ala Ala Val Ala Val Leu Cys145 150 155 160Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His 165 170 175Ser Leu Val Pro Leu Val Pro Met Glu Leu Gly Gly Thr Leu Arg Val 180 185 190Glu Arg Pro Ala Ser Val Thr Ser Leu Leu Ile Asp Ser Thr 195 200 20535411DNAEUCALYPTUS GUNNII 35actgaggtga cccggaagaa aaacagggta aagctatcgg gcactttggg ttatgtagcc 60ccagaatatg tcttggatgg taaattgact gataagagtg atgtctatgc ctttggagtt 120gtgcttttgg agctcctttt gagaagaagg cctcttgaga tagtagcacc cactcagtgc 180cagtctattg ttacatgggc catgcctcag ctgaccgacc gaactaagct tccagatatt 240gtggatcctg taattagaga tgcgatggat gtcaagcact tataccaggc agctgctgtt 300gctgttttgt gtctgcaacc agaaccgatc taccggccac tgataacgga tgtactccac 360tctctcattc cacttgtacc cgttgaactt gggggaacgc tgaagaccta g 41136136PRTEUCALYPTUS GUNNII 36Thr Glu Val Thr Arg Lys Lys Asn Arg Val Lys Leu Ser Gly Thr Leu1 5 10 15Gly Tyr Val Ala Pro Glu Tyr Val Leu Asp Gly Lys Leu Thr Asp Lys 20 25 30Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Leu Arg 35 40 45Arg Arg Pro Leu Glu Ile Val Ala Pro Thr Gln Cys Gln Ser Ile Val 50 55 60Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Thr Lys Leu Pro Asp Ile65 70 75 80Val Asp Pro Val Ile Arg Asp Ala Met Asp Val Lys His Leu Tyr Gln 85 90 95Ala Ala Ala Val Ala Val Leu Cys Leu Gln Pro Glu Pro Ile Tyr Arg 100 105 110Pro Leu Ile Thr Asp Val Leu His Ser Leu Ile Pro Leu Val Pro Val 115 120 125Glu Leu Gly Gly Thr Leu Lys Thr 130 13537522DNAFESTUCA ARUNDINACEA 37acgaggcctc gtgccatact tttggattca gatttcaatg ccaagatttc ggatttcggt 60cttgcagtgt caagtggaaa tcgcaccaaa ggtaatctga agctttccgg aactttgggc 120tatgttgctc ctgagtactt attagacggg aagttgacag agaagagtga tgtatatgcg 180ttcggagtag tacttcttga gcttttgtta ggaaggaggc caattgagaa gatggcccca 240tctcaatgcc aatcaattgt tacatgggcc atgcctcagc taattgacag atcaaagctc 300ccaaccataa ttgaccccgt gatcaggaac acgatggacc tgaagcactt gtaccaagtt 360gctgcagtgg ctgtgctctg tgtgcagcca gaaccaagtt ataggccact aatcacagat 420gtgctccact ctctgattcc cctggtgccc atggagctcg gagggtcact gagggctacc 480ttggaatcgc ctcgcgtatc acaacatcgt tctccctgct ga 52238173PRTFESTUCA ARUNDINACEA 38Thr Arg Pro Arg Ala Ile Leu Leu Asp Ser Asp Phe Asn Ala Lys Ile1 5 10 15Ser Asp Phe Gly Leu Ala Val Ser Ser Gly Asn Arg Thr Lys Gly Asn 20 25 30Leu Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu 35 40 45Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly Val Val 50 55 60Leu Leu Glu Leu Leu Leu Gly Arg Arg Pro Ile Glu Lys Met Ala Pro65 70 75 80Ser Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu Ile Asp 85 90 95Arg Ser Lys Leu Pro Thr Ile Ile Asp Pro Val Ile Arg Asn Thr Met 100 105 110Asp Leu Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val 115 120 125Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser 130 135 140Leu Ile Pro Leu Val Pro Met Glu Leu Gly Gly Ser Leu Arg Ala Thr145 150 155 160Leu Glu Ser Pro Arg Val Ser Gln His Arg Ser Pro Cys 165 17039740DNAGINKGO BILOBA 39cctttattga atagattgaa ctccttccgt ggttctagga gaaagggatg tgcatatata 60attgaatatt ctctgctgca agcagccaca aataatttta gtacaagtga catccttgga 120gagggtggtt ttgggtgtgt atacagagct aggttagatg atgatttctt tgctgctgtg 180aagaagttag atgagggcag caagcaggct gagtatgaat ttcagaatga agttgaacta 240atgagcaaaa tcagacatcc aaatcttgtt tctttgctgg ggttctgcat tcatgggaag 300actcggttgc tagtctacga gctcatgcaa aatggttctt tggaagacca attacatggg 360ccatctcatg gatccgcact tacatggtac ctgcgcatga aaatagccct tgattcagca 420aggggtctag aacacttgca cgagcactgc aatcctgctg tgattcatcg tgatttcaaa 480tcatcaaata tccttctgga tgcaagcttc aatgccaagc tttcagattt tggtcttgca 540gtaacagctg caggaggtat tggtaatgct aatgtcgagc tactgggcac tttgggatat 600gtagctccag aatacctgct tgatggcaag ttgacggaga aaagtgatgt ctatggattt 660ggagttgttc ttttggagct aattatggga agaaagccag ttgataaatc tgtggcaact 720gaaagtcaat cgctagtttc 74040247PRTGINKGO BILOBA 40Pro Leu Leu Asn Arg Leu Asn Ser Phe Arg Gly Ser Arg Arg Lys Gly1 5 10 15Cys Ala Tyr Ile Ile Glu Tyr Ser Leu Leu Gln Ala Ala Thr Asn Asn 20 25 30Phe Ser Thr Ser Asp Ile Leu Gly Glu Gly Gly Phe Gly Cys Val Tyr 35 40 45Arg Ala Arg Leu Asp Asp Asp Phe Phe Ala Ala Val Lys Lys Leu Asp 50 55 60Glu Gly Ser Lys Gln Ala Glu Tyr Glu Phe Gln Asn Glu Val Glu Leu65 70 75 80Met Ser Lys Ile Arg His Pro Asn Leu Val Ser Leu Leu Gly Phe Cys 85 90 95Ile His Gly Lys Thr Arg Leu Leu Val Tyr Glu Leu Met Gln Asn Gly 100 105 110Ser Leu Glu Asp Gln Leu His Gly Pro Ser His Gly Ser Ala Leu Thr 115 120 125Trp Tyr Leu Arg Met Lys Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu 130 135 140His Leu His Glu His Cys Asn Pro Ala Val Ile His Arg Asp Phe Lys145 150 155 160Ser Ser Asn Ile Leu Leu Asp Ala Ser Phe Asn Ala Lys Leu Ser Asp 165 170 175Phe Gly Leu Ala Val Thr Ala Ala Gly Gly Ile Gly Asn Ala Asn Val 180 185 190Glu Leu Leu Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp 195 200 205Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Gly Phe Gly Val Val Leu 210 215 220Leu Glu Leu Ile Met Gly Arg Lys Pro Val Asp Lys Ser Val Ala Thr225 230 235 240Glu Ser Gln Ser Leu Val Ser 245411254DNAGLYCINE MAX 41atgaaaatga agcttctcct catgcttctt cttcttgttc ttcttcttca ccaacccatt 60tgggctgcag accctcctgc ttcttctcct gctttatctc caggggagga gcagcatcac 120cggaataata aagtggtaat agctatcgtc gtagccacca ctgcacttgc tgcactcatt 180ttcagtttct tatgcttctg ggtttatcat cataccaagt atccaacaaa atccaaattc 240aaatccaaaa attttcgaag tccagatgca gagaagggga tcaccttagc accgtttgtg 300agtaaattca gttccatcaa gattgttggc atggacgggt atgttccaat aattgactat 360aagcaaatag aaaaaacgac caataatttt caagaaagta acatcttggg tgagggcggt 420tttggacgtg tttacaaggc ttgtttggat cataacttgg atgttgcagt caaaaaacta 480cattgtgaga ctcaacatgc tgagagagaa tttgagaacg aggtgaatat gttaagcaaa 540attcagcatc cgaatataat atctttactg ggttgtagca tggatggtta cacgaggctc 600gttgtctatg agctgatgca taatggatca ttggaagctc agttacatgg accttctcat 660ggctcggcat tgacttggca catgaggatg aagattgctc ttgacacagc aagaggatta 720gaatatctgc acgagcactg tcaccctgca gtgatccata gggatatgaa atcttctaat

780attctcttag atgcaaactt caatgccaag ctgtctgatt ttggtcttgc cttaactgat 840gggtcccaaa gcaagaagaa cattaaacta tcgggtacct tgggatacgt agcaccggag 900tatcttctag atggtaaatt aagtgataaa agtgatgtct atgcttttgg ggttgtgcta 960ttggagctcc tactaggaag gaagccagta gaaaaactgg taccagctca atgccaatct 1020attgtcacat gggccatgcc acacctcacg gacagatcca agcttccaag cattgtggat 1080ccagtgatta agaatacaat ggatcccaag cacttgtacc aggttgctgc tgtagctgtg 1140ctgtgcgtgc aaccagaacc tagttaccgt ccactgatca ttgatgttct tcactcactc 1200atccctcttg ttcccattga gcttggagga acactaagag tttcacaagt aatt 125442418PRTGLYCINE MAX 42Met Lys Met Lys Leu Leu Leu Met Leu Leu Leu Leu Val Leu Leu Leu1 5 10 15His Gln Pro Ile Trp Ala Ala Asp Pro Pro Ala Ser Ser Pro Ala Leu 20 25 30Ser Pro Gly Glu Glu Gln His His Arg Asn Asn Lys Val Val Ile Ala 35 40 45Ile Val Val Ala Thr Thr Ala Leu Ala Ala Leu Ile Phe Ser Phe Leu 50 55 60Cys Phe Trp Val Tyr His His Thr Lys Tyr Pro Thr Lys Ser Lys Phe65 70 75 80Lys Ser Lys Asn Phe Arg Ser Pro Asp Ala Glu Lys Gly Ile Thr Leu 85 90 95Ala Pro Phe Val Ser Lys Phe Ser Ser Ile Lys Ile Val Gly Met Asp 100 105 110Gly Tyr Val Pro Ile Ile Asp Tyr Lys Gln Ile Glu Lys Thr Thr Asn 115 120 125Asn Phe Gln Glu Ser Asn Ile Leu Gly Glu Gly Gly Phe Gly Arg Val 130 135 140Tyr Lys Ala Cys Leu Asp His Asn Leu Asp Val Ala Val Lys Lys Leu145 150 155 160His Cys Glu Thr Gln His Ala Glu Arg Glu Phe Glu Asn Glu Val Asn 165 170 175Met Leu Ser Lys Ile Gln His Pro Asn Ile Ile Ser Leu Leu Gly Cys 180 185 190Ser Met Asp Gly Tyr Thr Arg Leu Val Val Tyr Glu Leu Met His Asn 195 200 205Gly Ser Leu Glu Ala Gln Leu His Gly Pro Ser His Gly Ser Ala Leu 210 215 220Thr Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu225 230 235 240Glu Tyr Leu His Glu His Cys His Pro Ala Val Ile His Arg Asp Met 245 250 255Lys Ser Ser Asn Ile Leu Leu Asp Ala Asn Phe Asn Ala Lys Leu Ser 260 265 270Asp Phe Gly Leu Ala Leu Thr Asp Gly Ser Gln Ser Lys Lys Asn Ile 275 280 285Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp 290 295 300Gly Lys Leu Ser Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu305 310 315 320Leu Glu Leu Leu Leu Gly Arg Lys Pro Val Glu Lys Leu Val Pro Ala 325 330 335Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro His Leu Thr Asp Arg 340 345 350Ser Lys Leu Pro Ser Ile Val Asp Pro Val Ile Lys Asn Thr Met Asp 355 360 365Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln 370 375 380Pro Glu Pro Ser Tyr Arg Pro Leu Ile Ile Asp Val Leu His Ser Leu385 390 395 400Ile Pro Leu Val Pro Ile Glu Leu Gly Gly Thr Leu Arg Val Ser Gln 405 410 415Val Ile43702DNAHELIANTHUS ARGOPHYLLUS 43actcaagcat caaaatattg taaatctttt gggtattgtg ttcatgatga cacaaggttt 60ttggtctatg aaatgatgca tcaaggctct ttggactcac aattgcatgg accaactcat 120ggaaccgcat taacctggca tcgaagaatg aaagtcgcac ttgatattgc tcgaggatta 180gagtatcttc atgaacgatg caacccgcct gtgattcata gagatcttaa gtcatcgaac 240attttgctag attccaattt caatgctaaa atttcgaatt ttgcacttgc taccactgag 300ctccatgcga agaacaaagt taagctttcg gctacttctg gttatttggc tccggaatac 360ctatcagaag gtaaacttac cgataaaagc gacgtatatg cattcggagt agtacttctt 420gggcttttaa tcggtagaaa accagtggag aaaatgtcac catctttatt tcaatctatt 480gtcacatggg caatgcctca gttaacagac cggtcaaagc ttccaaacat cgttgaccct 540gtgattagag atacaatgga cctgaagcac ttatatcaag ttgctgctgt agccgtactt 600tgcgtgcaac ccgaaccaag ttacagaccg ttgattacag acgtactaca ctcattcatt 660ccactcgtac ccgttgatct tggagggtca ttaagagctt aa 70244233PRTHELIANTHUS ARGOPHYLLUS 44Thr Gln Ala Ser Lys Tyr Cys Lys Ser Phe Gly Tyr Cys Val His Asp1 5 10 15Asp Thr Arg Phe Leu Val Tyr Glu Met Met His Gln Gly Ser Leu Asp 20 25 30Ser Gln Leu His Gly Pro Thr His Gly Thr Ala Leu Thr Trp His Arg 35 40 45Arg Met Lys Val Ala Leu Asp Ile Ala Arg Gly Leu Glu Tyr Leu His 50 55 60Glu Arg Cys Asn Pro Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn65 70 75 80Ile Leu Leu Asp Ser Asn Phe Asn Ala Lys Ile Ser Asn Phe Ala Leu 85 90 95Ala Thr Thr Glu Leu His Ala Lys Asn Lys Val Lys Leu Ser Ala Thr 100 105 110Ser Gly Tyr Leu Ala Pro Glu Tyr Leu Ser Glu Gly Lys Leu Thr Asp 115 120 125Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Gly Leu Leu Ile 130 135 140Gly Arg Lys Pro Val Glu Lys Met Ser Pro Ser Leu Phe Gln Ser Ile145 150 155 160Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn 165 170 175Ile Val Asp Pro Val Ile Arg Asp Thr Met Asp Leu Lys His Leu Tyr 180 185 190Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr 195 200 205Arg Pro Leu Ile Thr Asp Val Leu His Ser Phe Ile Pro Leu Val Pro 210 215 220Val Asp Leu Gly Gly Ser Leu Arg Ala225 23045752DNAHELIANTHUS CILIARIS 45cgatcatttc gttgcggctg taaaaaactc catggtccag aaccagatgc ccaaaaaggg 60tttgagaatg aagtagattg gttaggtaaa ctcaagcatc aaaatattgt aaattttttg 120ggttattgtg ttcatgatga cacaaggttt ttggtctatg aaatgatgca tcaaggctct 180ttggactcac aattgcatgg accaactcat ggaaccgcat taacctggca tcgaagaatg 240aaagtcgcac ttgatattgc tcgaggatta gagtatcttc atgaacgatg caacccgcct 300gtgattcata gagatctcaa gtcatcgaac attttgctag attccaattt caatgctaaa 360atttcgaatt ttgcacttgc taccactgag ctccatgcga agaacaaagt taagctttcg 420ggtacttctg gttatttggc tccggaatac ctatccgaag gtaaacttac cgataaaagt 480gatgtatatg cattcggagt agtacttctt gagcttttaa tcggtagaaa accagtggag 540aaaatgtcac catctttatt tcaatctatt gtcacatggg caatgcctca gctaacagac 600cggtcaaagc ttccaaacat tgttgaccct gtgattagag atacaatgga cctgaagcac 660ttgtatcaag ttgctgctgt agccgtactt tgcgtgcaac ccgaaccaag ttacagaccg 720ttgattacag acgtactaca ctcattcatt cc 75246251PRTHELIANTHUS CILIARIS 46Arg Ser Phe Arg Cys Gly Cys Lys Lys Leu His Gly Pro Glu Pro Asp1 5 10 15Ala Gln Lys Gly Phe Glu Asn Glu Val Asp Trp Leu Gly Lys Leu Lys 20 25 30His Gln Asn Ile Val Asn Phe Leu Gly Tyr Cys Val His Asp Asp Thr 35 40 45Arg Phe Leu Val Tyr Glu Met Met His Gln Gly Ser Leu Asp Ser Gln 50 55 60Leu His Gly Pro Thr His Gly Thr Ala Leu Thr Trp His Arg Arg Met65 70 75 80Lys Val Ala Leu Asp Ile Ala Arg Gly Leu Glu Tyr Leu His Glu Arg 85 90 95Cys Asn Pro Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu 100 105 110Leu Asp Ser Asn Phe Asn Ala Lys Ile Ser Asn Phe Ala Leu Ala Thr 115 120 125Thr Glu Leu His Ala Lys Asn Lys Val Lys Leu Ser Gly Thr Ser Gly 130 135 140Tyr Leu Ala Pro Glu Tyr Leu Ser Glu Gly Lys Leu Thr Asp Lys Ser145 150 155 160Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Ile Gly Arg 165 170 175Lys Pro Val Glu Lys Met Ser Pro Ser Leu Phe Gln Ser Ile Val Thr 180 185 190Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Ile Val 195 200 205Asp Pro Val Ile Arg Asp Thr Met Asp Leu Lys His Leu Tyr Gln Val 210 215 220Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro225 230 235 240Leu Ile Thr Asp Val Leu His Ser Phe Ile Pro 245 25047630DNAHELIANTHUS EXILIS 47atgatgcatc aagactcttt ggactcacaa ttgcatggac caactcatgg aaccgcatta 60acctggcatc gaagaatgaa agtcgcactt gatattgctc gaggattaga gtatcttcat 120gaacgatgca acccgcctgt gattcataga gatctcaagt catcgaacat tttgctagat 180tccaatttca atgctaaaat ttcgaatttt gcacttgcta ccactgagct ccatgcgaag 240aacaaagtta agctttcggg tacttctggt tatttggctc cggaatacct atccgaaggt 300aaacttaccg ataaaagtga tgtatatgca ttcggagtag tacttcttga gcttttaatc 360ggtagaaaac cagtggagaa aatgtcacca tctttatttc aatctattgt cacatgggca 420atgcctcagc taacagaccg gtcaaagctt ccaaacattg ttgaccctgt gattagagat 480acaatggacc tgaagcactt gtatcaagtt gctgctgtag ccgtactttg cgtgcaaccc 540gaaccaagtt acagaccgtt gattacagac gtactacact cattcattcc actcgtaccc 600gttgatcttg gagggtcatt aagagcttaa 63048209PRTHELIANTHUS EXILIS 48Met Met His Gln Asp Ser Leu Asp Ser Gln Leu His Gly Pro Thr His1 5 10 15Gly Thr Ala Leu Thr Trp His Arg Arg Met Lys Val Ala Leu Asp Ile 20 25 30Ala Arg Gly Leu Glu Tyr Leu His Glu Arg Cys Asn Pro Pro Val Ile 35 40 45His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asn Phe Asn 50 55 60Ala Lys Ile Ser Asn Phe Ala Leu Ala Thr Thr Glu Leu His Ala Lys65 70 75 80Asn Lys Val Lys Leu Ser Gly Thr Ser Gly Tyr Leu Ala Pro Glu Tyr 85 90 95Leu Ser Glu Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly 100 105 110Val Val Leu Leu Glu Leu Leu Ile Gly Arg Lys Pro Val Glu Lys Met 115 120 125Ser Pro Ser Leu Phe Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu 130 135 140Thr Asp Arg Ser Lys Leu Pro Asn Ile Val Asp Pro Val Ile Arg Asp145 150 155 160Thr Met Asp Leu Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu 165 170 175Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu 180 185 190His Ser Phe Ile Pro Leu Val Pro Val Asp Leu Gly Gly Ser Leu Arg 195 200 205Ala49780DNAHORDEUM VULGARE 49aatttgagag gtgagctgga tttgcttcag aggattcagc attcgaatat agtgtccctt 60gtgggcttct gcattcatga ggagaaccgc ttcattgttt atgagctgat ggtgaatgga 120tcacttgaaa cacagcttca tgggccatca catggatcag ctctgagttg gcacattcgg 180atgaagattg ctcttgatac agcaagggga ttggagtatc ttcacgagca ctgcaatcca 240ccaatcatcc atagggatct gaagtcgtct aacatacttt tgaattcaga ctttaatgca 300aagatttcag attttggcct tgcagtgaca agtggaaatc gcagcaaagg gaatctgaag 360ctttccggta ctttgggtta tgttgcccct gagtacttac tagatgggaa gttgactgag 420aagagcgatg tatatgcatt tggagtagta cttcttgagc ttcttttggg aaggaggcca 480gttgagaaga tggcaccatc tcagtgtcaa tcaattgtta catgggccat gccccagcta 540attgacagat ccaagctccc taccataatc gaccccgtga tcagggacac gatggatcgg 600aagcacttgt accaagttgc tgcagtggct gtgctctgcg tgcagccaga accaagctac 660aggccactga tcacagatgt cctccactct ctgattcccc tggtgcccat ggaccttgga 720gggacgctga ggatcaaccc ggaatcgcct tgcacgacac gaaatcaatc tccctgctga 78050259PRTHORDEUM VULGARE 50Asn Leu Arg Gly Glu Leu Asp Leu Leu Gln Arg Ile Gln His Ser Asn1 5 10 15Ile Val Ser Leu Val Gly Phe Cys Ile His Glu Glu Asn Arg Phe Ile 20 25 30Val Tyr Glu Leu Met Val Asn Gly Ser Leu Glu Thr Gln Leu His Gly 35 40 45Pro Ser His Gly Ser Ala Leu Ser Trp His Ile Arg Met Lys Ile Ala 50 55 60Leu Asp Thr Ala Arg Gly Leu Glu Tyr Leu His Glu His Cys Asn Pro65 70 75 80Pro Ile Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asn Ser 85 90 95Asp Phe Asn Ala Lys Ile Ser Asp Phe Gly Leu Ala Val Thr Ser Gly 100 105 110Asn Arg Ser Lys Gly Asn Leu Lys Leu Ser Gly Thr Leu Gly Tyr Val 115 120 125Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Glu Lys Ser Asp Val 130 135 140Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Leu Gly Arg Arg Pro145 150 155 160Val Glu Lys Met Ala Pro Ser Gln Cys Gln Ser Ile Val Thr Trp Ala 165 170 175Met Pro Gln Leu Ile Asp Arg Ser Lys Leu Pro Thr Ile Ile Asp Pro 180 185 190Val Ile Arg Asp Thr Met Asp Arg Lys His Leu Tyr Gln Val Ala Ala 195 200 205Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile 210 215 220Thr Asp Val Leu His Ser Leu Ile Pro Leu Val Pro Met Asp Leu Gly225 230 235 240Gly Thr Leu Arg Ile Asn Pro Glu Ser Pro Cys Thr Thr Arg Asn Gln 245 250 255Ser Pro Cys51816DNAIPOMOEA BATATAS 51cgggggctct tatcactcat tgctgctgct actgcactgg gtacaagctt attgctcatg 60ggttgcttct ggatttatca tagaaagaaa atccacaaat ctcatgacat tattcatagc 120ccagatgtag ttaaaggtct tgcattatcc tcatatatta gcaaatacaa ctccttcaag 180tcgaattgtg tgaaacgaca tgtctcgttg tgggagtaca atacactcga gtcggccaca 240aatagttttc aagaaagcga gatcttgggt ggaggggggt tcgggcttgt gtacaaggga 300aaactagaag acaacttgta tgtagctgtg aagaggctgg aagttggaag acaaaacgca 360attaaagaat tcgaggctga aatagaggta ttgggcacga ttcagcaccc gaatataatt 420tcgttgttgg gatatagcat tcatgctgac acgaggctgc tagtttatga actgatgcag 480aatggatctc tggagtatca actacatgga ccttcccatg gatcagcatt agcgtggcat 540aatagattga aaatcgcact tgatacagca aggggattag aatatttaca tgaacattgc 600aaaccaccag ttatccatag agatctgaaa tcctccaata ttcttctaga tgccaacttc 660aatgccaaga tctcagattt tggtcttgct gtgcgcgatg gggctcaaaa caaaaataac 720attaagctct cgggaaccgt tggctatgta gctccagaat acctattaga tggaatacta 780acagataaaa gtgatgttta tggcttccga gttgta 81652272PRTIPOMOEA BATATAS 52Arg Gly Leu Leu Ser Leu Ile Ala Ala Ala Thr Ala Leu Gly Thr Ser1 5 10 15Leu Leu Leu Met Gly Cys Phe Trp Ile Tyr His Arg Lys Lys Ile His 20 25 30Lys Ser His Asp Ile Ile His Ser Pro Asp Val Val Lys Gly Leu Ala 35 40 45Leu Ser Ser Tyr Ile Ser Lys Tyr Asn Ser Phe Lys Ser Asn Cys Val 50 55 60Lys Arg His Val Ser Leu Trp Glu Tyr Asn Thr Leu Glu Ser Ala Thr65 70 75 80Asn Ser Phe Gln Glu Ser Glu Ile Leu Gly Gly Gly Gly Phe Gly Leu 85 90 95Val Tyr Lys Gly Lys Leu Glu Asp Asn Leu Tyr Val Ala Val Lys Arg 100 105 110Leu Glu Val Gly Arg Gln Asn Ala Ile Lys Glu Phe Glu Ala Glu Ile 115 120 125Glu Val Leu Gly Thr Ile Gln His Pro Asn Ile Ile Ser Leu Leu Gly 130 135 140Tyr Ser Ile His Ala Asp Thr Arg Leu Leu Val Tyr Glu Leu Met Gln145 150 155 160Asn Gly Ser Leu Glu Tyr Gln Leu His Gly Pro Ser His Gly Ser Ala 165 170 175Leu Ala Trp His Asn Arg Leu Lys Ile Ala Leu Asp Thr Ala Arg Gly 180 185 190Leu Glu Tyr Leu His Glu His Cys Lys Pro Pro Val Ile His Arg Asp 195 200 205Leu Lys Ser Ser Asn Ile Leu Leu Asp Ala Asn Phe Asn Ala Lys Ile 210 215 220Ser Asp Phe Gly Leu Ala Val Arg Asp Gly Ala Gln Asn Lys Asn Asn225 230 235 240Ile Lys Leu Ser Gly Thr Val Gly Tyr Val Ala Pro Glu Tyr Leu Leu 245 250 255Asp Gly Ile Leu Thr Asp Lys Ser Asp Val Tyr Gly Phe Arg Val Val 260 265 27053867DNALACTUCA SATIVA 53ggggatatac gtgtagaatc agcaacaaat aacttcggtg aaagcgagat attaggcgta 60ggtggatttg gatgcgtgta taaagctcga ctcgatgata atttgcatgt agctgttaaa 120agattagatg gtattagtca agacgccatt aaagaattcc agacggaggt ggatctattg 180agtaaaattc atcatccgaa tatcatcacc ttattgggat attgtgttaa tgatgaaacc 240aagcttcttg tttatgaact gatgcataat ggatctttag aaactcaatt acatgggcct 300tccagtggat ccaatttaac atggcattgc aggatgaaga ttgctctaga tacagcaaga 360ggattagaat atttgcatga gaactgcaaa ccatcggtga ttcatagaga tctgaaatca 420tctaatatcc ttctggattc cagcttcaat gctaagcttt cagattttgg tcttgctata 480atggatgggg cccagaacaa aaacaacatt aagctttcag ggacattggg ttatgtagct 540cccgagtatc ttttagatgg

aaaattgacg gataaaagtg acgtgtatgc gtttggagtt 600gtgcttttag agcttttact tggaaggcga cctgtagaaa aattagcaga gtcgcaatgc 660caatctattg tcacttgggc tatgccacaa ttaacagaca gatcaaagct tccgaatatt 720gtagatcccg tgatcagata cacaatggat ctcaagcacc tgtaccaagt tgctgcggtg 780gctgtgttat gtgtacaacc cggaccaagc taccggccat ttataaaccg acgtcttgca 840ttctctgatc cctcttgttc cccgtga 86754288PRTLACTUCA SATIVA 54Gly Asp Ile Arg Val Glu Ser Ala Thr Asn Asn Phe Gly Glu Ser Glu1 5 10 15Ile Leu Gly Val Gly Gly Phe Gly Cys Val Tyr Lys Ala Arg Leu Asp 20 25 30Asp Asn Leu His Val Ala Val Lys Arg Leu Asp Gly Ile Ser Gln Asp 35 40 45Ala Ile Lys Glu Phe Gln Thr Glu Val Asp Leu Leu Ser Lys Ile His 50 55 60His Pro Asn Ile Ile Thr Leu Leu Gly Tyr Cys Val Asn Asp Glu Thr65 70 75 80Lys Leu Leu Val Tyr Glu Leu Met His Asn Gly Ser Leu Glu Thr Gln 85 90 95Leu His Gly Pro Ser Ser Gly Ser Asn Leu Thr Trp His Cys Arg Met 100 105 110Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu Glu Tyr Leu His Glu Asn 115 120 125Cys Lys Pro Ser Val Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu 130 135 140Leu Asp Ser Ser Phe Asn Ala Lys Leu Ser Asp Phe Gly Leu Ala Ile145 150 155 160Met Asp Gly Ala Gln Asn Lys Asn Asn Ile Lys Leu Ser Gly Thr Leu 165 170 175Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys 180 185 190Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Leu Gly 195 200 205Arg Arg Pro Val Glu Lys Leu Ala Glu Ser Gln Cys Gln Ser Ile Val 210 215 220Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Ile225 230 235 240Val Asp Pro Val Ile Arg Tyr Thr Met Asp Leu Lys His Leu Tyr Gln 245 250 255Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Gly Pro Ser Tyr Arg 260 265 270Pro Phe Ile Asn Arg Arg Leu Ala Phe Ser Asp Pro Ser Cys Ser Pro 275 280 28555804DNAMEDICAGO TRUNCATULA 55aagttgaact gtgaatgtca atatgctgag agagaatttg agaatgaggt ggatttgtta 60agtaaaattc aacatccaaa tgtaatttct ctactgggct gtagcagtaa tgaggattca 120aggtttattg tctatgagtt gatgcaaaat ggatcattgg aaactcaatt acatggacca 180tctcatggct cagcattgac ttggcatatg aggatgaaga ttgctcttga cacagctaga 240ggtttaaaat atctgcatga gcactgctac cctgcagtga tccatagaga tctgaaatct 300tctaatattc ttttagatgc aaacttcaat gccaagcttt ctgattttgg tcttgcaata 360actgatgggt cccaaaacaa gaataacatc aagctttcag gcacattggg gtatgttgcc 420ccggagtatc ttttagatgg taaattgaca gataaaagtg atgtgtatgc ttttggagtt 480gtgcttcttg agcttctatt aggaagaaag cctgtggaaa aacttacacc atctcaatgc 540cagtctattg tcacatgggc catgccacag ctcacagaca gatccaagct tccaaacatt 600gtggataatg tgattaagaa tacaatggat cctaagcact tataccaggt tgctgctgtg 660gctgtattat gtgtgcaacc agagccgtgc taccgccctt tgattgcaga tgttctacac 720tccctcatcc ctcttgtacc tgttgagctt ggaggaacac tcagagttgc acaagtgacg 780cagcaaccta agaattctag ttaa 80456267PRTMEDICAGO TRUNCATULA 56Lys Leu Asn Cys Glu Cys Gln Tyr Ala Glu Arg Glu Phe Glu Asn Glu1 5 10 15Val Asp Leu Leu Ser Lys Ile Gln His Pro Asn Val Ile Ser Leu Leu 20 25 30Gly Cys Ser Ser Asn Glu Asp Ser Arg Phe Ile Val Tyr Glu Leu Met 35 40 45Gln Asn Gly Ser Leu Glu Thr Gln Leu His Gly Pro Ser His Gly Ser 50 55 60Ala Leu Thr Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg65 70 75 80Gly Leu Lys Tyr Leu His Glu His Cys Tyr Pro Ala Val Ile His Arg 85 90 95Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ala Asn Phe Asn Ala Lys 100 105 110Leu Ser Asp Phe Gly Leu Ala Ile Thr Asp Gly Ser Gln Asn Lys Asn 115 120 125Asn Ile Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu 130 135 140Leu Asp Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val145 150 155 160Val Leu Leu Glu Leu Leu Leu Gly Arg Lys Pro Val Glu Lys Leu Thr 165 170 175Pro Ser Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu Thr 180 185 190Asp Arg Ser Lys Leu Pro Asn Ile Val Asp Asn Val Ile Lys Asn Thr 195 200 205Met Asp Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys 210 215 220Val Gln Pro Glu Pro Cys Tyr Arg Pro Leu Ile Ala Asp Val Leu His225 230 235 240Ser Leu Ile Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Val 245 250 255Ala Gln Val Thr Gln Gln Pro Lys Asn Ser Ser 260 26557636DNANICOTIANA TABACUM 57cagttgcatg gacctcctcg tggatcagct ttgaattggc atcttcgcat ggaaattgca 60ttggatgtgg ctaggggact agaatacctc catgagcgct gtaacccccc tgtaatccat 120agagatctca aatcgtctaa tgttctattg gattcctact tcaatgcaaa gctttctgac 180ttttggccta gctatagctg gatggaactt aaacaagagc accgtaaagt ctttcgggaa 240ctctgggata tgtggctcca gagttacctc ttagatggga aattaactga taagagtgat 300gtctatgctt tcggcattat acttctggag cttctaatgg ggagaagacc attggagaaa 360ctagcaggag ctcagtgcca atctatcgtc acatgggcaa tgccacagct tactgacagg 420tcaaagctcc caaatattgt tgatcctgtc atcagaaacg gaatgggcct caagcacttg 480tatcaagttg ctgctgtagc cgtgctatgt gtacaaccag aaccaagtta ccgaccactg 540ataacagatg tcctgcactc cttcattccc cttgtaccaa ttgagcttgg tgggtccttg 600agagttgtgg attctgcatt atctgttaac gcataa 63658211PRTNICOTIANA TABACUM 58Gln Leu His Gly Pro Pro Arg Gly Ser Ala Leu Asn Trp His Leu Arg1 5 10 15Met Glu Ile Ala Leu Asp Val Ala Arg Gly Leu Glu Tyr Leu His Glu 20 25 30Arg Cys Asn Pro Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn Val 35 40 45Leu Leu Asp Ser Tyr Phe Asn Ala Lys Leu Ser Asp Phe Trp Pro Ser 50 55 60Tyr Ser Trp Met Glu Leu Lys Gln Glu His Arg Lys Val Phe Arg Glu65 70 75 80Leu Trp Asp Met Trp Leu Gln Ser Tyr Leu Leu Asp Gly Lys Leu Thr 85 90 95Asp Lys Ser Asp Val Tyr Ala Phe Gly Ile Ile Leu Leu Glu Leu Leu 100 105 110Met Gly Arg Arg Pro Leu Glu Lys Leu Ala Gly Ala Gln Cys Gln Ser 115 120 125Ile Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro 130 135 140Asn Ile Val Asp Pro Val Ile Arg Asn Gly Met Gly Leu Lys His Leu145 150 155 160Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser 165 170 175Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Phe Ile Pro Leu Val 180 185 190Pro Ile Glu Leu Gly Gly Ser Leu Arg Val Val Asp Ser Ala Leu Ser 195 200 205Val Asn Ala 210591437DNAORYZA SATIVA 59atggagatgg cgctaactcc attgccgctc ctgtgttcgt ccgtcttgtt cttggtgcta 60tcttcgtgct cgttggccaa tgggagggat acgccttctt cttcttcttc ttcttcttct 120tcttcttctt cttcttcttc ttcttcttct tcttcttctt ctccggcgac gtctactgtg 180gccaccggca tttccgccgc cgccgccgcc gccgccaatg ggacggccgc cttgtcttcg 240gcagttccgg cgcctccgcc tgttgtgatc gtagtgcacc accatttcca ccgcgagctg 300gtcatcgccg ccgtcctcgc ctgcatcgcc accgtcacga tcttcctttc cacgctctac 360gcttggacac tatggcggcg atctcgccgg agcaccggcg gcaaggtcac caggagctca 420gacgcagcga aggggatcaa gctggtgccg atcttgagca ggttcaactc ggtgaagatg 480agcaggaaga ggctggttgg gatgttcgag tacccgtcgc tggaggcagc gacagagaag 540ttcagcgaga gcaacatgct cggtgtcggc gggtttggcc gcgtctacaa ggcggcgttc 600gacgccggag ttaccgcggc ggtgaagcgg ctcgacggcg gcgggcccga ctgcgagaag 660gaattcgaga atgagctgga tttgcttggc aggatcaggc accccaacat tgtgtccctc 720ttgggcttct gtatccatga ggggaatcac tacattgttt atgagctgat ggagaaggga 780tcactggaaa cacagcttca tgggtcttca catggatcaa ctctgagctg gcacatccgg 840atgaagatcg cccttgacac ggccagggga ttagagtacc ttcatgagca ctgcagtcca 900ccagtgatcc atagggatct gaaatcgtct aacatacttt tggattcaga cttcaatgct 960aagattgcag attttggtct tgctgtgtct agtgggagtg tcaacaaagg gagtgtgaag 1020ctctccggga ccttgggtta tgtagctcct gagtacttgt tggatgggaa gttgactgaa 1080aagagcgatg tatacgcgtt cggagtagtg cttctagagc tccttatggg gaggaagcct 1140gttgagaaga tgtcaccatc tcagtgccaa tcaattgtga catgggcaat gccacagttg 1200accgacagat cgaagctccc cagcatagtt gacccagtga tcaaggacac catggatcca 1260aaacacctgt accaagttgc agcagtggct gttctatgcg tgcaggctga accaagctac 1320aggccactga tcacagatgt gctccactct cttgttcctc tagtgccgac ggagctcgga 1380ggaacactaa gagctggaga gccaccttcc ccgaacctga ggaattctcc atgctga 143760478PRTORYZA SATIVA 60Met Glu Met Ala Leu Thr Pro Leu Pro Leu Leu Cys Ser Ser Val Leu1 5 10 15Phe Leu Val Leu Ser Ser Cys Ser Leu Ala Asn Gly Arg Asp Thr Pro 20 25 30Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 35 40 45Ser Ser Ser Ser Ser Ser Pro Ala Thr Ser Thr Val Ala Thr Gly Ile 50 55 60Ser Ala Ala Ala Ala Ala Ala Ala Asn Gly Thr Ala Ala Leu Ser Ser65 70 75 80Ala Val Pro Ala Pro Pro Pro Val Val Ile Val Val His His His Phe 85 90 95His Arg Glu Leu Val Ile Ala Ala Val Leu Ala Cys Ile Ala Thr Val 100 105 110Thr Ile Phe Leu Ser Thr Leu Tyr Ala Trp Thr Leu Trp Arg Arg Ser 115 120 125Arg Arg Ser Thr Gly Gly Lys Val Thr Arg Ser Ser Asp Ala Ala Lys 130 135 140Gly Ile Lys Leu Val Pro Ile Leu Ser Arg Phe Asn Ser Val Lys Met145 150 155 160Ser Arg Lys Arg Leu Val Gly Met Phe Glu Tyr Pro Ser Leu Glu Ala 165 170 175Ala Thr Glu Lys Phe Ser Glu Ser Asn Met Leu Gly Val Gly Gly Phe 180 185 190Gly Arg Val Tyr Lys Ala Ala Phe Asp Ala Gly Val Thr Ala Ala Val 195 200 205Lys Arg Leu Asp Gly Gly Gly Pro Asp Cys Glu Lys Glu Phe Glu Asn 210 215 220Glu Leu Asp Leu Leu Gly Arg Ile Arg His Pro Asn Ile Val Ser Leu225 230 235 240Leu Gly Phe Cys Ile His Glu Gly Asn His Tyr Ile Val Tyr Glu Leu 245 250 255Met Glu Lys Gly Ser Leu Glu Thr Gln Leu His Gly Ser Ser His Gly 260 265 270Ser Thr Leu Ser Trp His Ile Arg Met Lys Ile Ala Leu Asp Thr Ala 275 280 285Arg Gly Leu Glu Tyr Leu His Glu His Cys Ser Pro Pro Val Ile His 290 295 300Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asp Phe Asn Ala305 310 315 320Lys Ile Ala Asp Phe Gly Leu Ala Val Ser Ser Gly Ser Val Asn Lys 325 330 335Gly Ser Val Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr 340 345 350Leu Leu Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly 355 360 365Val Val Leu Leu Glu Leu Leu Met Gly Arg Lys Pro Val Glu Lys Met 370 375 380Ser Pro Ser Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu385 390 395 400Thr Asp Arg Ser Lys Leu Pro Ser Ile Val Asp Pro Val Ile Lys Asp 405 410 415Thr Met Asp Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu 420 425 430Cys Val Gln Ala Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu 435 440 445His Ser Leu Val Pro Leu Val Pro Thr Glu Leu Gly Gly Thr Leu Arg 450 455 460Ala Gly Glu Pro Pro Ser Pro Asn Leu Arg Asn Ser Pro Cys465 470 47561891DNAPHYSCOMITRELLA 61tactctcttt tacaaactgc tacgaacaac ttcagctcct ccaatttgct gggcgaggga 60agtttcgggc atgtgtataa agcgagactc gattatgatg tctatgccgc tgtaaagaga 120cttaccagcg taggaaaaca gccccaaaaa gaactccagg gagaggtgga tctgatgtgc 180aagataagac atcccaactt ggtggctctc ctgggctatt caaatgacgg cccagagccc 240ttggttgtgt acgagctcat gcagaatggt tcacttcatg atcagcttca tggcccctca 300tgcgggagtg cactcacctg gtacctacga ctaaagattg ctcttgaagc tgccagcaga 360ggactggagc acctgcatga aagctgcaag cctgcaataa tccacagaga cttcaaggca 420tccaacatcc tcttggacgc cagcttcaat gcgaaggtgt ccgactttgg tatagcggta 480gctctggagg aaggtggcgt ggtgaaagac gacgtacaag tgcaaggcac cttcgggtac 540attgctcctg agtacctgat ggacgggaca ttgacagaga agagtgatgt ttacggattt 600ggagtagtat tgcttgagct gctgacaggc agactgccca ttgatacgtc cttaccactc 660ggatcgcaat ctctagtgac atgggtaaca cccatactaa ctaaccgagc aaagctgatg 720gaagttatcg accccaccct tcaagatacg ctgaacgtga agcaacttca ccaggtggcc 780gcagtggcag tcctttgcgt ccaagcggaa cccagctacc gccctctcat cgccgacgtg 840gttcagtcac tggctccgct ggtgcctcaa gagctcggcg gcgcattgcg a 89162297PRTPHYSCOMITRELLA 62Tyr Ser Leu Leu Gln Thr Ala Thr Asn Asn Phe Ser Ser Ser Asn Leu1 5 10 15Leu Gly Glu Gly Ser Phe Gly His Val Tyr Lys Ala Arg Leu Asp Tyr 20 25 30Asp Val Tyr Ala Ala Val Lys Arg Leu Thr Ser Val Gly Lys Gln Pro 35 40 45Gln Lys Glu Leu Gln Gly Glu Val Asp Leu Met Cys Lys Ile Arg His 50 55 60Pro Asn Leu Val Ala Leu Leu Gly Tyr Ser Asn Asp Gly Pro Glu Pro65 70 75 80Leu Val Val Tyr Glu Leu Met Gln Asn Gly Ser Leu His Asp Gln Leu 85 90 95His Gly Pro Ser Cys Gly Ser Ala Leu Thr Trp Tyr Leu Arg Leu Lys 100 105 110Ile Ala Leu Glu Ala Ala Ser Arg Gly Leu Glu His Leu His Glu Ser 115 120 125Cys Lys Pro Ala Ile Ile His Arg Asp Phe Lys Ala Ser Asn Ile Leu 130 135 140Leu Asp Ala Ser Phe Asn Ala Lys Val Ser Asp Phe Gly Ile Ala Val145 150 155 160Ala Leu Glu Glu Gly Gly Val Val Lys Asp Asp Val Gln Val Gln Gly 165 170 175Thr Phe Gly Tyr Ile Ala Pro Glu Tyr Leu Met Asp Gly Thr Leu Thr 180 185 190Glu Lys Ser Asp Val Tyr Gly Phe Gly Val Val Leu Leu Glu Leu Leu 195 200 205Thr Gly Arg Leu Pro Ile Asp Thr Ser Leu Pro Leu Gly Ser Gln Ser 210 215 220Leu Val Thr Trp Val Thr Pro Ile Leu Thr Asn Arg Ala Lys Leu Met225 230 235 240Glu Val Ile Asp Pro Thr Leu Gln Asp Thr Leu Asn Val Lys Gln Leu 245 250 255His Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Ala Glu Pro Ser 260 265 270Tyr Arg Pro Leu Ile Ala Asp Val Val Gln Ser Leu Ala Pro Leu Val 275 280 285Pro Gln Glu Leu Gly Gly Ala Leu Arg 290 295631065DNAPICEA 63acctcagatg cctatagggg tattccactc atgcctctcc tgaatcgttt gaactcccgt 60atttccaaga agaagggatg tgcaactgca attgaatatt ctaagctgca agcagctaca 120aataacttca gcagcaataa cattcttgga gagggtggat ttgcgtgtgt atacaaggcc 180atgtttgatg atgattcctt tgctgctgtg aagaagctag atgagggtag cagacaggct 240gagcatgaat ttcagaatga agtggagctg atgagcaaaa tccgacatcc aaaccttgtt 300tctttgcttg ggttctgctc tcatgaaaat acacggttct tagtatatga tctgatgcag 360aatggctctt tggaagacca attacatggg ccatctcacg gatctgcact tacatggttt 420ttgcgcataa agatagcact tgattcagca aggggtctag aacacttgca tgagcactgc 480aaccctgcag tgattcatcg agatttcaaa tcatcaaata ttcttcttga tgcaagcttc 540aacgccaagc tttcagattt tggtcttgca gtaacaagtg caggatgtgc tggcaataca 600aatattgatc tagtagggac attgggatat gtagctccag aatacctact tgatggtaaa 660ttgacagaga aaagtgatgt ctatgcatat ggagttgttt tgttggagct actttttgga 720agaaagccaa ttgataaatc tctaccaagt gaatgccaat ctctcatttc ttgggcaatg 780ccacagctaa cagatagaga aaagctccca actatagtag accccatgat caaaggcaca 840atgaacttga aacacctata tcaagtagca gctgttgcaa tgctatgtgt gcagccagaa 900cccagttaca ggccattaat agctgacgtt gtgcactctc tcattcctct cgtaccaata 960gaactcgggg gaactttaaa gctctctaat gcacgaccca ctgagatgaa gttatttact 1020tcttcccaat gcagtgttga gattgcttcc aacccaaaat tgtga 106564354PRTPICEA 64Thr Ser Asp Ala Tyr Arg Gly Ile Pro Leu Met Pro Leu Leu Asn Arg1 5 10 15Leu Asn Ser Arg Ile Ser Lys Lys Lys Gly Cys Ala Thr Ala Ile Glu 20 25 30Tyr Ser Lys Leu Gln Ala Ala Thr

Asn Asn Phe Ser Ser Asn Asn Ile 35 40 45Leu Gly Glu Gly Gly Phe Ala Cys Val Tyr Lys Ala Met Phe Asp Asp 50 55 60Asp Ser Phe Ala Ala Val Lys Lys Leu Asp Glu Gly Ser Arg Gln Ala65 70 75 80Glu His Glu Phe Gln Asn Glu Val Glu Leu Met Ser Lys Ile Arg His 85 90 95Pro Asn Leu Val Ser Leu Leu Gly Phe Cys Ser His Glu Asn Thr Arg 100 105 110Phe Leu Val Tyr Asp Leu Met Gln Asn Gly Ser Leu Glu Asp Gln Leu 115 120 125His Gly Pro Ser His Gly Ser Ala Leu Thr Trp Phe Leu Arg Ile Lys 130 135 140Ile Ala Leu Asp Ser Ala Arg Gly Leu Glu His Leu His Glu His Cys145 150 155 160Asn Pro Ala Val Ile His Arg Asp Phe Lys Ser Ser Asn Ile Leu Leu 165 170 175Asp Ala Ser Phe Asn Ala Lys Leu Ser Asp Phe Gly Leu Ala Val Thr 180 185 190Ser Ala Gly Cys Ala Gly Asn Thr Asn Ile Asp Leu Val Gly Thr Leu 195 200 205Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Glu Lys 210 215 220Ser Asp Val Tyr Ala Tyr Gly Val Val Leu Leu Glu Leu Leu Phe Gly225 230 235 240Arg Lys Pro Ile Asp Lys Ser Leu Pro Ser Glu Cys Gln Ser Leu Ile 245 250 255Ser Trp Ala Met Pro Gln Leu Thr Asp Arg Glu Lys Leu Pro Thr Ile 260 265 270Val Asp Pro Met Ile Lys Gly Thr Met Asn Leu Lys His Leu Tyr Gln 275 280 285Val Ala Ala Val Ala Met Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg 290 295 300Pro Leu Ile Ala Asp Val Val His Ser Leu Ile Pro Leu Val Pro Ile305 310 315 320Glu Leu Gly Gly Thr Leu Lys Leu Ser Asn Ala Arg Pro Thr Glu Met 325 330 335Lys Leu Phe Thr Ser Ser Gln Cys Ser Val Glu Ile Ala Ser Asn Pro 340 345 350Lys Leu65596DNAPINUS 65aattcggcac gaggagaaca cttgcacgag cactgcaacc ctgcagtgat tcaccgagat 60ttcaaatcat caaatattct tcttgatgca agcttcaacg ccaagctttc agattttggt 120cttgcagtaa aaagtgcagg atgtgctggt aacacaaata ttgatctagt agggacattg 180ggatatgtag ctccagaata catgcttgat ggtaaattga cagagaaaag tgatgtctat 240gcatatggag ttgttttgtt agagctactt tttggaagaa agccaattga taaatctcta 300ccaagtgaat gccaatctct catttcttgg gcaatgccac agctaacaga tagagaaaag 360ctcccgacta taatagatcc catgatcaaa ggcgcaatga acttgaaaca cctatatcaa 420gtggcagctg ttgcagtgct atgtgtgcag ccagaaccca gttacaggcc attaatagct 480gacgttgtgc actctctcat tcctctcgta ccagtagaac ttgggggaac attaaagtca 540tcacccactg agatgaagtc atttgcttct tcccaatgca gtgcccacgt tgcttc 59666199PRTPINUS 66Asn Ser Ala Arg Gly Glu His Leu His Glu His Cys Asn Pro Ala Val1 5 10 15Ile His Arg Asp Phe Lys Ser Ser Asn Ile Leu Leu Asp Ala Ser Phe 20 25 30Asn Ala Lys Leu Ser Asp Phe Gly Leu Ala Val Lys Ser Ala Gly Cys 35 40 45Ala Gly Asn Thr Asn Ile Asp Leu Val Gly Thr Leu Gly Tyr Val Ala 50 55 60Pro Glu Tyr Met Leu Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr65 70 75 80Ala Tyr Gly Val Val Leu Leu Glu Leu Leu Phe Gly Arg Lys Pro Ile 85 90 95Asp Lys Ser Leu Pro Ser Glu Cys Gln Ser Leu Ile Ser Trp Ala Met 100 105 110Pro Gln Leu Thr Asp Arg Glu Lys Leu Pro Thr Ile Ile Asp Pro Met 115 120 125Ile Lys Gly Ala Met Asn Leu Lys His Leu Tyr Gln Val Ala Ala Val 130 135 140Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Ala145 150 155 160Asp Val Val His Ser Leu Ile Pro Leu Val Pro Val Glu Leu Gly Gly 165 170 175Thr Leu Lys Ser Ser Pro Thr Glu Met Lys Ser Phe Ala Ser Ser Gln 180 185 190Cys Ser Ala His Val Ala Ser 195671377DNAPOPULUS 67atgttcttgt ttcctaaaac agttcctatt tggttttttc atctgtgtct agtagcagtt 60catgccatac aagaagaccc acctgtccct tcaccatctc cctctctcat ttctcctatt 120tcaacttcaa tggctgcctt ctctccaggg gttgaatcgg aaatgggaat caaagaccac 180ccccagcatg atgacctcca caggaaaata atcttgttgc tcactgttgc ttgttgcata 240cttgttatca tccttctttc tttgtgttct tgtttcattt actataagaa gtcctcacaa 300aagaaaaaag ctactcggtg ttcagatgtg gagaaagggc tttcattggc accatttttg 360ggcaaattca gttccttgaa aatggttagt aataggggat ctgtttcatt aattgagtat 420aagatactag agaaaggaac aaacaatttt ggcgatgata aattgttggg aaagggagga 480tttggacgtg tatataaggc tgtaatggaa gatgactcaa gtgctgcagt caagaaacta 540gactgcgcaa ctgatgatgc gcagagagaa tttgagaatg aggtggattt gttaagcaaa 600tttcaccatc caaatataat ttctattgtg ggttttagtg ttcatgagga gatggggttc 660attatttatg agttaatgcc aaatgggtgc cttgaagatc tactgcatgg accttctcgt 720ggatcttcac taaattggca tttaaggttg aaaattgctc ttgatacagc aagaggatta 780gaatatctgc atgaattctg caagccagca gtgatccata gagatctgaa atcatcgaat 840attcttttgg acgccaactt caatgccaag ctgtcagatt ttggtcttgc tgtagctgat 900agctctcata acaagaaaaa gctcaagctt tcaggcactg tgggttatgt agccccagag 960tatatgttag atggtgaatt gacggataag agtgatgtct atgcttttgg agttgtgctt 1020ctagagcttc tattaggaag aaggcctgta gaaaaactga caccagctca ttgccaatct 1080atagtaacat gggccatgcc tcagctcact aacagagctg tgcttccaac ccttgtggat 1140cctgtgatca gagattcagt agatgagaag tacttgttcc aggttgcagc agtagccgtg 1200ttgtgtattc aaccagagcc aagttaccgc cctctcataa cagatgttgt gcactctctc 1260gtcccattag ttcctcttga gcttggaggg acactaagag ttccacagcc tacaactccc 1320agaggtcaac gacaaggccc atcaaagaaa ctgtttttgg atggtgctgc ctctgct 137768459PRTPOPULUS 68Met Phe Leu Phe Pro Lys Thr Val Pro Ile Trp Phe Phe His Leu Cys1 5 10 15Leu Val Ala Val His Ala Ile Gln Glu Asp Pro Pro Val Pro Ser Pro 20 25 30Ser Pro Ser Leu Ile Ser Pro Ile Ser Thr Ser Met Ala Ala Phe Ser 35 40 45Pro Gly Val Glu Ser Glu Met Gly Ile Lys Asp His Pro Gln His Asp 50 55 60Asp Leu His Arg Lys Ile Ile Leu Leu Leu Thr Val Ala Cys Cys Ile65 70 75 80Leu Val Ile Ile Leu Leu Ser Leu Cys Ser Cys Phe Ile Tyr Tyr Lys 85 90 95Lys Ser Ser Gln Lys Lys Lys Ala Thr Arg Cys Ser Asp Val Glu Lys 100 105 110Gly Leu Ser Leu Ala Pro Phe Leu Gly Lys Phe Ser Ser Leu Lys Met 115 120 125Val Ser Asn Arg Gly Ser Val Ser Leu Ile Glu Tyr Lys Ile Leu Glu 130 135 140Lys Gly Thr Asn Asn Phe Gly Asp Asp Lys Leu Leu Gly Lys Gly Gly145 150 155 160Phe Gly Arg Val Tyr Lys Ala Val Met Glu Asp Asp Ser Ser Ala Ala 165 170 175Val Lys Lys Leu Asp Cys Ala Thr Asp Asp Ala Gln Arg Glu Phe Glu 180 185 190Asn Glu Val Asp Leu Leu Ser Lys Phe His His Pro Asn Ile Ile Ser 195 200 205Ile Val Gly Phe Ser Val His Glu Glu Met Gly Phe Ile Ile Tyr Glu 210 215 220Leu Met Pro Asn Gly Cys Leu Glu Asp Leu Leu His Gly Pro Ser Arg225 230 235 240Gly Ser Ser Leu Asn Trp His Leu Arg Leu Lys Ile Ala Leu Asp Thr 245 250 255Ala Arg Gly Leu Glu Tyr Leu His Glu Phe Cys Lys Pro Ala Val Ile 260 265 270His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ala Asn Phe Asn 275 280 285Ala Lys Leu Ser Asp Phe Gly Leu Ala Val Ala Asp Ser Ser His Asn 290 295 300Lys Lys Lys Leu Lys Leu Ser Gly Thr Val Gly Tyr Val Ala Pro Glu305 310 315 320Tyr Met Leu Asp Gly Glu Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe 325 330 335Gly Val Val Leu Leu Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys 340 345 350Leu Thr Pro Ala His Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln 355 360 365Leu Thr Asn Arg Ala Val Leu Pro Thr Leu Val Asp Pro Val Ile Arg 370 375 380Asp Ser Val Asp Glu Lys Tyr Leu Phe Gln Val Ala Ala Val Ala Val385 390 395 400Leu Cys Ile Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val 405 410 415Val His Ser Leu Val Pro Leu Val Pro Leu Glu Leu Gly Gly Thr Leu 420 425 430Arg Val Pro Gln Pro Thr Thr Pro Arg Gly Gln Arg Gln Gly Pro Ser 435 440 445Lys Lys Leu Phe Leu Asp Gly Ala Ala Ser Ala 450 45569693DNASACCHARUM OFFICINARUM 69gctgctgcgg tgaagagatt ggatggtggg gctggggcac atgattgcga gaaggaattc 60gagaatgagt tagatttgct tggaaagatt cggcatccga acattgtgtc ccttgtgggc 120ttctgtattc atgaggagaa ccgtttcatt gtttatgagc tgatagagaa tgggtcgttg 180gattcacaac ttcatgggcc atcacatggt tcagctctga gctggcatat tcggatgaag 240attgctcttg acacggcaag gggattagag tacctgcatg agcactgcaa cccaccagtt 300atccataggg atctgaagtc atctaacata cttttagatt cagacttcag tgctaagatt 360tcagattttg gccttgcggt gattagtggg aatcacagca aagggaattt aaagctttct 420gggactatgg gctatgtggc ccctgagtac ttattggatg ggaagttgac tgagaagagc 480gatgtatatg cgtttggggt ggtacttcta gaacttctac tgggaaggaa acctgttgag 540aagatggcac aatctcaatg ccaatcaatt gttacatggg ccatgcctca gctaactgat 600agatccaaac tccctaacat aattgatccc atgatcaaga acacaatgga tctgaaacac 660ttgtaccaag ttgctgcaat ggctgtgctc tga 69370230PRTSACCHARUM OFFICINARUM 70Ala Ala Ala Val Lys Arg Leu Asp Gly Gly Ala Gly Ala His Asp Cys1 5 10 15Glu Lys Glu Phe Glu Asn Glu Leu Asp Leu Leu Gly Lys Ile Arg His 20 25 30Pro Asn Ile Val Ser Leu Val Gly Phe Cys Ile His Glu Glu Asn Arg 35 40 45Phe Ile Val Tyr Glu Leu Ile Glu Asn Gly Ser Leu Asp Ser Gln Leu 50 55 60His Gly Pro Ser His Gly Ser Ala Leu Ser Trp His Ile Arg Met Lys65 70 75 80Ile Ala Leu Asp Thr Ala Arg Gly Leu Glu Tyr Leu His Glu His Cys 85 90 95Asn Pro Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu 100 105 110Asp Ser Asp Phe Ser Ala Lys Ile Ser Asp Phe Gly Leu Ala Val Ile 115 120 125Ser Gly Asn His Ser Lys Gly Asn Leu Lys Leu Ser Gly Thr Met Gly 130 135 140Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Glu Lys Ser145 150 155 160Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Leu Gly Arg 165 170 175Lys Pro Val Glu Lys Met Ala Gln Ser Gln Cys Gln Ser Ile Val Thr 180 185 190Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Ile Ile 195 200 205Asp Pro Met Ile Lys Asn Thr Met Asp Leu Lys His Leu Tyr Gln Val 210 215 220Ala Ala Met Ala Val Leu225 23071414DNATRIPHYSARIA VERSICOLOR 71accctcggtt atgtagctcc tgagtatctg ttagatggta agttaacaga gaaaagcgat 60gtgtatgggt ttggagtagt gttactcgag cttctgcttg ggaagaagcc tatggagaaa 120gtggcaacaa cagcaactca gtgccagatg atagtcacat ggaccatgcc tcagctcact 180gacagaacga aacttccgaa tatcgtggat ccggtgatca gaaactccat ggatttaaag 240cacttgtacc aggttgctgc tgtggcagta ttgtgtgtgc agccagaacc gagttatcgg 300ccattgataa ctgatatttt gcattctctt gtgccccttg tccctgttga gcttggtggg 360acgctcagga actcgataac aatggctaca acaacaatat ctcctgaaag ctaa 41472137PRTTRIPHYSARIA VERSICOLOR 72Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr1 5 10 15Glu Lys Ser Asp Val Tyr Gly Phe Gly Val Val Leu Leu Glu Leu Leu 20 25 30Leu Gly Lys Lys Pro Met Glu Lys Val Ala Thr Thr Ala Thr Gln Cys 35 40 45Gln Met Ile Val Thr Trp Thr Met Pro Gln Leu Thr Asp Arg Thr Lys 50 55 60Leu Pro Asn Ile Val Asp Pro Val Ile Arg Asn Ser Met Asp Leu Lys65 70 75 80His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu 85 90 95Pro Ser Tyr Arg Pro Leu Ile Thr Asp Ile Leu His Ser Leu Val Pro 100 105 110Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Asn Ser Ile Thr Met 115 120 125Ala Thr Thr Thr Ile Ser Pro Glu Ser 130 135731140DNATRITICUM AESTIVUMmisc_feature(1000)..(1000)n is a, c, g, or tmisc_feature(1080)..(1080)n is a, c, g, or tmisc_feature(1088)..(1088)n is a, c, g, or t 73cggcacgagg ggctggtggc catgatcgag tacccgtcgc tggaggcggc gacgggcaag 60ttcagcgaga gcaacgtgct cggcgtcggc gggttcggct gcgtctacaa ggcggcgttc 120gacggcggcg ccaccgccgc cgtgaagagg ctcgaaggcg gcgagccgga ctgcgagaag 180gagttcgaga atgagctgga cttgcttggc aggatcaggc acccaaacat agtgtccctc 240ctgggcttct gcgtccatgg tggcaatcac tacattgttt atgagctcat ggagaaggga 300tcattggaga cacaactgca tgggccttca catggatcgg ctatgagctg gcacgtccgg 360atgaagatcg cgctcgacac ggcgagggga ttagagtatc ttcatgagca ctgcaatcca 420ccagtcatcc atagggatct gaaatcgtct aatatactct tggattcaga cttcaatgct 480aagattgcag attttggcct tgcagtgaca agtgggaatc ttgacaaagg gaacctgaag 540atctctggga ccttgggata tgtagctccc gagtacttat tagatgggaa gttgaccgag 600aagagcgacg tctacgcgtt tggagtagtg cttctagagc tcctgatggg gaggaagcct 660gttgagaaga tgtcaccatc tcagtgccaa tcaattgtgt catgggccat gcctcagcta 720accgacagat cgaagctacc caacatcatc gacccggtga tcaaggacac aatggaccca 780aagcatttat accaagttgc ggcggtggcc gttctatgcg tgcagcccga accgagttac 840agaccgctga taacagacgt tctccactcc cttgttcctc tggtacccgc ggatctcggg 900gggaacgctc agagttacag agccgcattc tccacaccaa atgtaccatc cctcttgaga 960agtgatccta caagtttcgt cgaagcgggg aaagcgaatn tatacggtcc agcggtagat 1020ggctgttatt ttggtactta tatctcaccc tgtcctgctg cttatcttag gatgagtgan 1080gagctccnac ctgctgcttt tgctggttgg gcagagagaa tacagttctg gttaggattg 114074380PRTTRITICUM AESTIVUMmisc_feature(334)..(334)Xaa can be any naturally occurring amino acidmisc_feature(360)..(360)Xaa can be any naturally occurring amino acidmisc_feature(363)..(363)Xaa can be any naturally occurring amino acid 74Arg His Glu Gly Leu Val Ala Met Ile Glu Tyr Pro Ser Leu Glu Ala1 5 10 15Ala Thr Gly Lys Phe Ser Glu Ser Asn Val Leu Gly Val Gly Gly Phe 20 25 30Gly Cys Val Tyr Lys Ala Ala Phe Asp Gly Gly Ala Thr Ala Ala Val 35 40 45Lys Arg Leu Glu Gly Gly Glu Pro Asp Cys Glu Lys Glu Phe Glu Asn 50 55 60Glu Leu Asp Leu Leu Gly Arg Ile Arg His Pro Asn Ile Val Ser Leu65 70 75 80Leu Gly Phe Cys Val His Gly Gly Asn His Tyr Ile Val Tyr Glu Leu 85 90 95Met Glu Lys Gly Ser Leu Glu Thr Gln Leu His Gly Pro Ser His Gly 100 105 110Ser Ala Met Ser Trp His Val Arg Met Lys Ile Ala Leu Asp Thr Ala 115 120 125Arg Gly Leu Glu Tyr Leu His Glu His Cys Asn Pro Pro Val Ile His 130 135 140Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asp Phe Asn Ala145 150 155 160Lys Ile Ala Asp Phe Gly Leu Ala Val Thr Ser Gly Asn Leu Asp Lys 165 170 175Gly Asn Leu Lys Ile Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr 180 185 190Leu Leu Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly 195 200 205Val Val Leu Leu Glu Leu Leu Met Gly Arg Lys Pro Val Glu Lys Met 210 215 220Ser Pro Ser Gln Cys Gln Ser Ile Val Ser Trp Ala Met Pro Gln Leu225 230 235 240Thr Asp Arg Ser Lys Leu Pro Asn Ile Ile Asp Pro Val Ile Lys Asp 245 250 255Thr Met Asp Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu 260 265 270Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu 275 280 285His Ser Leu Val Pro Leu Val Pro Ala Asp Leu Gly Gly Asn Ala Gln 290 295 300Ser Tyr Arg Ala Ala Phe Ser Thr Pro Asn Val Pro Ser Leu Leu Arg305 310 315 320Ser Asp Pro Thr Ser Phe Val Glu Ala Gly Lys Ala Asn Xaa Tyr Gly 325 330 335Pro Ala Val Asp Gly Cys Tyr Phe Gly Thr Tyr Ile Ser Pro Cys Pro 340 345

350Ala Ala Tyr Leu Arg Met Ser Xaa Glu Leu Xaa Pro Ala Ala Phe Ala 355 360 365Gly Trp Ala Glu Arg Ile Gln Phe Trp Leu Gly Leu 370 375 38075978DNAVITIS VINIFERA 75atgaaagtga ttgggagaaa gggttatgtc tcttttattg attataaggt actagaaact 60gcaacaaaca attttcagga aagtaatatc ctgggtgagg gcgggtttgg ttgcgtctac 120aaggcgcggt tggatgataa ctcccatgtg gctgtgaaga agatagatgg tagaggccag 180gatgctgaga gagaatttga gaatgaggtg gatttgttga ctaaaattca gcacccaaat 240ataatttctc tcctgggtta cagcagtcat gaggagtcaa agtttcttgt ctatgagctg 300atgcagaatg gatctctgga aactgaattg cacggacctt ctcatggatc atctctaact 360tggcatattc gaatgaaaat cgctctggat gcagcaagag gattagagta tctacatgag 420cactgcaacc caccagtcat ccatagagat cttaaatcat ctaatattct tctggattca 480aacttcaatg ccaagctttc ggattttggt ctagctgtaa ttgatgggcc tcaaaacaag 540aacaacttga agctttcagg caccctgggt tatctagctc ctgagtatct tttagatggt 600aaactgactg ataagagtga tgtgtatgca tttggagtgg tgcttctaga gctactactg 660ggaagaaagc ctgtggaaaa actggcacca gctcaatgcc agtccattgt cacatgggcc 720atgccacagc tgactgacag atcaaagctc ccaggcatcg ttgaccctgt ggtcagagac 780acgatggatc taaagcattt ataccaagtt gctgctgtag ctgtgctatg tgtgcaacca 840gaaccaagtt accggccatt gataacagat gttctgcact ccctcatccc actcgttcca 900gttgagttgg gagggatgct aaaagttacc cagcaagcgc cgcctatcaa caccactgca 960ccttctgctg gaggttga 97876325PRTVITIS VINIFERA 76Met Lys Val Ile Gly Arg Lys Gly Tyr Val Ser Phe Ile Asp Tyr Lys1 5 10 15Val Leu Glu Thr Ala Thr Asn Asn Phe Gln Glu Ser Asn Ile Leu Gly 20 25 30Glu Gly Gly Phe Gly Cys Val Tyr Lys Ala Arg Leu Asp Asp Asn Ser 35 40 45His Val Ala Val Lys Lys Ile Asp Gly Arg Gly Gln Asp Ala Glu Arg 50 55 60Glu Phe Glu Asn Glu Val Asp Leu Leu Thr Lys Ile Gln His Pro Asn65 70 75 80Ile Ile Ser Leu Leu Gly Tyr Ser Ser His Glu Glu Ser Lys Phe Leu 85 90 95Val Tyr Glu Leu Met Gln Asn Gly Ser Leu Glu Thr Glu Leu His Gly 100 105 110Pro Ser His Gly Ser Ser Leu Thr Trp His Ile Arg Met Lys Ile Ala 115 120 125Leu Asp Ala Ala Arg Gly Leu Glu Tyr Leu His Glu His Cys Asn Pro 130 135 140Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser145 150 155 160Asn Phe Asn Ala Lys Leu Ser Asp Phe Gly Leu Ala Val Ile Asp Gly 165 170 175Pro Gln Asn Lys Asn Asn Leu Lys Leu Ser Gly Thr Leu Gly Tyr Leu 180 185 190Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys Ser Asp Val 195 200 205Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Leu Gly Arg Lys Pro 210 215 220Val Glu Lys Leu Ala Pro Ala Gln Cys Gln Ser Ile Val Thr Trp Ala225 230 235 240Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Gly Ile Val Asp Pro 245 250 255Val Val Arg Asp Thr Met Asp Leu Lys His Leu Tyr Gln Val Ala Ala 260 265 270Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile 275 280 285Thr Asp Val Leu His Ser Leu Ile Pro Leu Val Pro Val Glu Leu Gly 290 295 300Gly Met Leu Lys Val Thr Gln Gln Ala Pro Pro Ile Asn Thr Thr Ala305 310 315 320Pro Ser Ala Gly Gly 325771377DNAZEA MAYS 77atgccgccgc catcgccgct cctccgttcc tccgccttcg tcgtcttgct gctcctggtg 60tgtcgcccgt tgttggtcgc caatgggagg gccacgccgc cttctccggg atggccaccg 120gcggctcagc ccgcgctgca gcctgcaccc accgccagcg gcggcgtggc ctccgtgctt 180ccttcggccg tggcgcctcc tcccttaggt gtggttgtgg cggagaggca ccaccacctc 240agcagggagc tcgtcgctgc cattatcctc tcatccgtcg ccagcgtcgt gatccccatt 300gccgcgctgt atgccttctt gctgtggcga cgatcacggc gagccctggt ggattccaag 360gacacccaga gcatagatac cgcaaggatt gcttttgcgc cgatgttgaa cagctttggc 420tcgtacaaga ctaccaagaa gagtgccgcg gcgatgatgg attacacatc tttggaggca 480gcgacagaaa acttcagtga gagcaatgtc cttggatttg gtgggtttgg gtctgtgtac 540aaagccaatt ttgatgggag gtttgctgct gcggtgaaga gactggatgg tggggcacat 600gattgcaaga aggaattcga gaatgagcta gacttgcttg ggaagattcg acatccgaac 660atcgtgtccc ttgtgggctt ctgcattcat gaggagaacc gtttcgttgt ttatgagctg 720atggagagtg ggtcgttgga ttcgcaactt catgggccat cacatggttc agctctgagc 780tggcatattc ggatgaagat tgctctcgac acagcaaggg gattagagta cctgcatgag 840cactgcaacc caccggttat ccatagggat cttaagtcat ctaacatact tttagattca 900gacttcagcg ctaagatttc agactttggc ctggcagtga ctagtgggaa tcacagcaaa 960gggaatttaa agctttctgg gactatgggc tatgtggctc ctgagtactt attagatggg 1020aagctgactg agaagagcga tgtatacgcg tttggggtag tacttctaga actcctgctg 1080ggaaggaaac ctgtcgagaa gatggcacaa tctcagtgcc gatcaatcgt tacatgggcc 1140atgcctcagc taactgatag atccaagctc ccgaacataa ttgatcccat gatcaagaac 1200acaatggatc tgaaacactt gtaccaagtt gctgcagtgg ccgtgctctg cgtgcagcca 1260gagccgagtt acaggccact gatcaccgac gtgcttcact cactggtacc tctagtgccc 1320acggagcttg gaggaacgct gaggatcggc ccggaatcgc cctacctacg ctactaa 137778458PRTZEA MAYS 78Met Pro Pro Pro Ser Pro Leu Leu Arg Ser Ser Ala Phe Val Val Leu1 5 10 15Leu Leu Leu Val Cys Arg Pro Leu Leu Val Ala Asn Gly Arg Ala Thr 20 25 30Pro Pro Ser Pro Gly Trp Pro Pro Ala Ala Gln Pro Ala Leu Gln Pro 35 40 45Ala Pro Thr Ala Ser Gly Gly Val Ala Ser Val Leu Pro Ser Ala Val 50 55 60Ala Pro Pro Pro Leu Gly Val Val Val Ala Glu Arg His His His Leu65 70 75 80Ser Arg Glu Leu Val Ala Ala Ile Ile Leu Ser Ser Val Ala Ser Val 85 90 95Val Ile Pro Ile Ala Ala Leu Tyr Ala Phe Leu Leu Trp Arg Arg Ser 100 105 110Arg Arg Ala Leu Val Asp Ser Lys Asp Thr Gln Ser Ile Asp Thr Ala 115 120 125Arg Ile Ala Phe Ala Pro Met Leu Asn Ser Phe Gly Ser Tyr Lys Thr 130 135 140Thr Lys Lys Ser Ala Ala Ala Met Met Asp Tyr Thr Ser Leu Glu Ala145 150 155 160Ala Thr Glu Asn Phe Ser Glu Ser Asn Val Leu Gly Phe Gly Gly Phe 165 170 175Gly Ser Val Tyr Lys Ala Asn Phe Asp Gly Arg Phe Ala Ala Ala Val 180 185 190Lys Arg Leu Asp Gly Gly Ala His Asp Cys Lys Lys Glu Phe Glu Asn 195 200 205Glu Leu Asp Leu Leu Gly Lys Ile Arg His Pro Asn Ile Val Ser Leu 210 215 220Val Gly Phe Cys Ile His Glu Glu Asn Arg Phe Val Val Tyr Glu Leu225 230 235 240Met Glu Ser Gly Ser Leu Asp Ser Gln Leu His Gly Pro Ser His Gly 245 250 255Ser Ala Leu Ser Trp His Ile Arg Met Lys Ile Ala Leu Asp Thr Ala 260 265 270Arg Gly Leu Glu Tyr Leu His Glu His Cys Asn Pro Pro Val Ile His 275 280 285Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asp Phe Ser Ala 290 295 300Lys Ile Ser Asp Phe Gly Leu Ala Val Thr Ser Gly Asn His Ser Lys305 310 315 320Gly Asn Leu Lys Leu Ser Gly Thr Met Gly Tyr Val Ala Pro Glu Tyr 325 330 335Leu Leu Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly 340 345 350Val Val Leu Leu Glu Leu Leu Leu Gly Arg Lys Pro Val Glu Lys Met 355 360 365Ala Gln Ser Gln Cys Arg Ser Ile Val Thr Trp Ala Met Pro Gln Leu 370 375 380Thr Asp Arg Ser Lys Leu Pro Asn Ile Ile Asp Pro Met Ile Lys Asn385 390 395 400Thr Met Asp Leu Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu 405 410 415Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu 420 425 430His Ser Leu Val Pro Leu Val Pro Thr Glu Leu Gly Gly Thr Leu Arg 435 440 445Ile Gly Pro Glu Ser Pro Tyr Leu Arg Tyr 450 455791188DNAZEA MAYS 79atgttgctcg cgtgtcctgc agtgatcatc gtggagcgcc accgtcattt ccaccgtgag 60ctagtcatcg cctccatcct cgcctcaatc gccatggtcg cgattatcct ctccacgctg 120tacgcgtgga tcccgcgcag gcggtcccgc cggctgcccc gcggcatgag cgcagacacc 180gcgaggggga tcatgctggc gccgatcctg agcaagttca actcgctcaa gacgagcagg 240aaggggctcg tggcgatgat cgagtacccg tcgctggagg cagcgacagg ggggttcagt 300gagagcaacg tgctcggcgt aggcggcttc ggttgcgtct acaaggcagt cttcgatggc 360ggcgttaccg cggcggtcaa gaggctggag ggaggtggcc ctgagtgcga gaaggaattc 420gagaatgagc tggatctgct tggcaggatt cggcacccca acatcgtgtc cctgctgggc 480ttttgtgttc acgaggggaa tcactacatt gtttatgagc tcatggagaa gggatccctg 540gacacacagc tgcatggggc ctcacatgga tcagcgctga cctggcatat ccggatgaag 600atcgcactcg acatggccag gggattagaa tacctccatg agcactgcag tccaccagtg 660atccataggg atctgaagtc atctaacata cttttagatt ctgacttcaa tgctaagatt 720tcagattttg gtcttgcagt gaccagtggg aacattgaca agggaagcat gaagctttct 780gggaccttgg gttatgtggc ccctgagtac ctattagatg ggaagctgac tgaaaagagt 840gacgtatatg catttggagt ggtgcttctt gagctactaa tgggaaggaa gcctgtcgag 900aagatgagtc aaactcagtg ccaatcaatt gtgacgtggg ccatgccgca gctgactgac 960agaacaaaac ttcccaacat agttgaccca gtgatcaggg acaccatgga tccaaagcat 1020ttgtaccaag tggcagcagt ggcagttcta tgtgtgcaac cagaaccaag ttacagaccg 1080ctgattactg atgttctcca ctctcttgtc cctctagtcc ctgtggagct cggagggaca 1140ctgagggttg tagagccacc ttccccaaac ctaaaacatt ctccttgt 118880396PRTZEA MAYS 80Met Leu Leu Ala Cys Pro Ala Val Ile Ile Val Glu Arg His Arg His1 5 10 15Phe His Arg Glu Leu Val Ile Ala Ser Ile Leu Ala Ser Ile Ala Met 20 25 30Val Ala Ile Ile Leu Ser Thr Leu Tyr Ala Trp Ile Pro Arg Arg Arg 35 40 45Ser Arg Arg Leu Pro Arg Gly Met Ser Ala Asp Thr Ala Arg Gly Ile 50 55 60Met Leu Ala Pro Ile Leu Ser Lys Phe Asn Ser Leu Lys Thr Ser Arg65 70 75 80Lys Gly Leu Val Ala Met Ile Glu Tyr Pro Ser Leu Glu Ala Ala Thr 85 90 95Gly Gly Phe Ser Glu Ser Asn Val Leu Gly Val Gly Gly Phe Gly Cys 100 105 110Val Tyr Lys Ala Val Phe Asp Gly Gly Val Thr Ala Ala Val Lys Arg 115 120 125Leu Glu Gly Gly Gly Pro Glu Cys Glu Lys Glu Phe Glu Asn Glu Leu 130 135 140Asp Leu Leu Gly Arg Ile Arg His Pro Asn Ile Val Ser Leu Leu Gly145 150 155 160Phe Cys Val His Glu Gly Asn His Tyr Ile Val Tyr Glu Leu Met Glu 165 170 175Lys Gly Ser Leu Asp Thr Gln Leu His Gly Ala Ser His Gly Ser Ala 180 185 190Leu Thr Trp His Ile Arg Met Lys Ile Ala Leu Asp Met Ala Arg Gly 195 200 205Leu Glu Tyr Leu His Glu His Cys Ser Pro Pro Val Ile His Arg Asp 210 215 220Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asp Phe Asn Ala Lys Ile225 230 235 240Ser Asp Phe Gly Leu Ala Val Thr Ser Gly Asn Ile Asp Lys Gly Ser 245 250 255Met Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu 260 265 270Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly Val Val 275 280 285Leu Leu Glu Leu Leu Met Gly Arg Lys Pro Val Glu Lys Met Ser Gln 290 295 300Thr Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu Thr Asp305 310 315 320Arg Thr Lys Leu Pro Asn Ile Val Asp Pro Val Ile Arg Asp Thr Met 325 330 335Asp Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val 340 345 350Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser 355 360 365Leu Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Val Val 370 375 380Glu Pro Pro Ser Pro Asn Leu Lys His Ser Pro Cys385 390 395811086DNAGOSSYPIUM 81atgaagaaga agcttgtgct gcatctgctt cttttccttg tttgtgctct tgaaaacatt 60gttttggccg tacaaggccc tgcttcatca cccatttcta ctcccatctc tgcttcaatg 120gctgccttct ctccagctgg gattcaactt ggaggtgagg agcacaagaa aatggatcca 180accaagaaaa tgttattagc tctcattctt gcttgctctt cattgggtgc aattatctct 240tccttgttct gtttatggat ttattacagg aagaattcaa gcaaatcctc taaaaatggc 300gctaagagct cagatggtga aaaagggaat ggtttggcac catatttggg taaattcaag 360tctatgagga cggtttccaa agagggttat gcttcgttta tggactataa gatacttgaa 420aaagctacaa acaagttcca tcatggtaac attctgggtg agggtggatt tggatgtgtt 480tacaaggctc aattcaatga tggttcttat gctgctgtta agaagttgga ctgtgcaagc 540caagatgctg aaaaagaata tgagaatgag gtgggtttgc tatgtagatt taagcattcc 600aatataattt cactgttggg ttatagcagt gataacgata caaggtttat tgtttatgag 660ttgatggaaa atggttcttt ggaaactcaa ttacatggac cttctcatgg ttcatcatta 720acttggcata ggaggatgaa aattgctttg gatacagcaa gaggattaga atatctacat 780gagcattgca atccaccagt catccataga gatctgaaat catctaatat acttttggat 840ttggacttca atgcaaagct ttcagatttt ggtcttgcag taactgatgc ggcaacaaac 900aagaataact tgaagctttc gggtacttta ggttatctag ctccagaata ccttttagat 960ggtaaattaa cagataagag tgatgtttat gcattcggtg ttgtgctgct cgaacttcta 1020ttgggacgaa aggctgttga aaaattatca caactcagtg ccaatcttag gtccatttgg 1080gcatag 108682361PRTGOSSYPIUM 82Met Lys Lys Lys Leu Val Leu His Leu Leu Leu Phe Leu Val Cys Ala1 5 10 15Leu Glu Asn Ile Val Leu Ala Val Gln Gly Pro Ala Ser Ser Pro Ile 20 25 30Ser Thr Pro Ile Ser Ala Ser Met Ala Ala Phe Ser Pro Ala Gly Ile 35 40 45Gln Leu Gly Gly Glu Glu His Lys Lys Met Asp Pro Thr Lys Lys Met 50 55 60Leu Leu Ala Leu Ile Leu Ala Cys Ser Ser Leu Gly Ala Ile Ile Ser65 70 75 80Ser Leu Phe Cys Leu Trp Ile Tyr Tyr Arg Lys Asn Ser Ser Lys Ser 85 90 95Ser Lys Asn Gly Ala Lys Ser Ser Asp Gly Glu Lys Gly Asn Gly Leu 100 105 110Ala Pro Tyr Leu Gly Lys Phe Lys Ser Met Arg Thr Val Ser Lys Glu 115 120 125Gly Tyr Ala Ser Phe Met Asp Tyr Lys Ile Leu Glu Lys Ala Thr Asn 130 135 140Lys Phe His His Gly Asn Ile Leu Gly Glu Gly Gly Phe Gly Cys Val145 150 155 160Tyr Lys Ala Gln Phe Asn Asp Gly Ser Tyr Ala Ala Val Lys Lys Leu 165 170 175Asp Cys Ala Ser Gln Asp Ala Glu Lys Glu Tyr Glu Asn Glu Val Gly 180 185 190Leu Leu Cys Arg Phe Lys His Ser Asn Ile Ile Ser Leu Leu Gly Tyr 195 200 205Ser Ser Asp Asn Asp Thr Arg Phe Ile Val Tyr Glu Leu Met Glu Asn 210 215 220Gly Ser Leu Glu Thr Gln Leu His Gly Pro Ser His Gly Ser Ser Leu225 230 235 240Thr Trp His Arg Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu 245 250 255Glu Tyr Leu His Glu His Cys Asn Pro Pro Val Ile His Arg Asp Leu 260 265 270Lys Ser Ser Asn Ile Leu Leu Asp Leu Asp Phe Asn Ala Lys Leu Ser 275 280 285Asp Phe Gly Leu Ala Val Thr Asp Ala Ala Thr Asn Lys Asn Asn Leu 290 295 300Lys Leu Ser Gly Thr Leu Gly Tyr Leu Ala Pro Glu Tyr Leu Leu Asp305 310 315 320Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu 325 330 335Leu Glu Leu Leu Leu Gly Arg Lys Ala Val Glu Lys Leu Ser Gln Leu 340 345 350Ser Ala Asn Leu Arg Ser Ile Trp Ala 355 360831089DNASOLANUM LYCOPERSICUMmisc_feature(1024)..(1024)n is a, c, g, or t 83ggagtgggaa ttgagaagca gccacccacc cacccaccct atggataaaa atagaaggct 60gttgatagca ctcattgtag cttctactgc attaggacta atctttatct tcatcatttt 120attctggatt tttcacaaaa gatttcacac ctcagatgtt gtgaagggaa tgagtaggaa 180aacattggtt tctttaatgg actacaacat acttgaatca gccaccaaca aatttaaaga 240aactgagatt ttaggtgagg ggggttttgg atgtgtgtac aaagctaaat tggaagacaa 300tttttatgta gctgtcaaga aactaaccca aaattccatt aaagaatttg agactgagtt 360agagttgttg agtcaaatgc aacatcccaa tattatttca ttgttgggat attgcatcca 420cagtgaaaca agattgcttg tctatgaact catgcaaaat ggatcactag aaactcaatt 480acatgggcct tcccgtggat cagcattaac ttggcatcgc aggataaaaa ttgcccttga 540tgcagcaaga

ggaatagaat atttacatga gcagcgccat ccccctgtaa ttcatagaga 600tctgaaatca tctaatattc ttttagattc caacttcaat gcaaaggtaa aactttttat 660gtagaaatta tactaggact agttttccct ctattaatct tgtgttgtga ttaattttag 720ctgtcagatt ttggtcttgc tgtgttgagt ggggctcaaa acaaaaacaa tatcaagctt 780tctggaacta taggttatgt agcgcctgaa tacatgttag atggaaaatt aagtgataaa 840agtgatgttt atggttttgg agtagtactt ttggagctgt tattgggaag gcggcctgta 900gaaaaggagg cagccactga atgtcagtct atagtgacat gggccatgcc tcagctgaca 960gatagatcaa agcttccaaa cattgttgat cctgtcatac aaaacacaat ggatttaaag 1020catntgtatc aggttgctgc aggtgctcta ttatgtgttc agccagagcc aagctatcgt 1080cccgtataa 108984875DNAAQUILEGIA 84gagtatcagt tattggaagc tgcaactgac aattttagtg agagtaatat tttgggagaa 60ggtggatttg gatgtgttta caaagcatgt tttgataaca actttctcgc tgctgtcaag 120agaatggatg ttggtgggca agatgcagaa agagaatttg agaaagaagt agatttgttg 180aatagaattc agcatccgga tataatttcc ctgttgggtt attgtattca tgatgagaca 240aggttcatca tttatgaact aatgcagaac ggatctttgg aaagacaatt acatggacct 300tctcatggat cggctttaac ttggcatatc cggatgaaaa ttgcacttga tacagcaaga 360gcattagaat atctccatga gaattgcaac cctcctgtga tccacagaga tctgaaatca 420tccaatatac ttttggattc taatttcaag gccaagattt cagattttgg tcttgctgta 480atttctggga gtcaaaacaa gaacaacatt aagctttcag gcactcttgg ttatgttgct 540ccagaatatc tgttagatgg taaattgact gacaaaagtg atgtctatgc ttttggggtt 600atccttctag aactcctaat gggaagaaaa cctgtagaga aaatgacacg aactcagtgt 660caatctatcg ttacatgggc catgcctcaa ctcactgata gatcaaagct accaaacatt 720gttgatcctg tgattaaaaa cacaatggat ttgaagcatt tgttccaagt tgctgctgta 780gctgtactgt gtgtacaacc agaaccaagt taccggccat taatcacaga tgtccttcac 840tccctcgtac cccttgttcc tgtcgatctt ggagg 87585292PRTAQUILEGIA 85Glu Tyr Gln Leu Leu Glu Ala Ala Thr Asp Asn Phe Ser Glu Ser Asn1 5 10 15Ile Leu Gly Glu Gly Gly Phe Gly Cys Val Tyr Lys Ala Cys Phe Asp 20 25 30Asn Asn Phe Leu Ala Ala Val Lys Arg Met Asp Val Gly Gly Gln Asp 35 40 45Ala Glu Arg Glu Phe Glu Lys Glu Val Asp Leu Leu Asn Arg Ile Gln 50 55 60His Pro Asp Ile Ile Ser Leu Leu Gly Tyr Cys Ile His Asp Glu Thr65 70 75 80Arg Phe Ile Ile Tyr Glu Leu Met Gln Asn Gly Ser Leu Glu Arg Gln 85 90 95Leu His Gly Pro Ser His Gly Ser Ala Leu Thr Trp His Ile Arg Met 100 105 110Lys Ile Ala Leu Asp Thr Ala Arg Ala Leu Glu Tyr Leu His Glu Asn 115 120 125Cys Asn Pro Pro Val Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu 130 135 140Leu Asp Ser Asn Phe Lys Ala Lys Ile Ser Asp Phe Gly Leu Ala Val145 150 155 160Ile Ser Gly Ser Gln Asn Lys Asn Asn Ile Lys Leu Ser Gly Thr Leu 165 170 175Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys 180 185 190Ser Asp Val Tyr Ala Phe Gly Val Ile Leu Leu Glu Leu Leu Met Gly 195 200 205Arg Lys Pro Val Glu Lys Met Thr Arg Thr Gln Cys Gln Ser Ile Val 210 215 220Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Ile225 230 235 240Val Asp Pro Val Ile Lys Asn Thr Met Asp Leu Lys His Leu Phe Gln 245 250 255Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg 260 265 270Pro Leu Ile Thr Asp Val Leu His Ser Leu Val Pro Leu Val Pro Val 275 280 285Asp Leu Gly Gly 29086696DNACENTAUREA MACULOSA 86tgtgctcatg atgagaccaa actacttgtt tacgaactta tgcacaatgg ttcgttagaa 60actcaattac acggtccttc ttgtggatcc aatttaacat ggcattgtcg gatgaaaatt 120gcgctagata tagcgagagg attggaatat ttacatgaac actgcaaacc atctgtgatt 180catagagatt tgaagtcatc taacatcctt ttggattcaa aattcaatgc caagctttcg 240gatttcggtc ttgctgtgat gaacggtgcc aataccaaaa acattaagct ttcggggacg 300ttgggttacg tagctcccga gtatctttta aatgggaaat tgaccgataa aagtgacgtc 360tacgcattcg gagttgtact tttagagctt ctactcaaaa ggcggcctgt cgaaaaacta 420gcaccatccg agtgccagtc catcgtcact tgggctatgc cgcaactaac agacagaaca 480aagcttccga gtgttataga tcccgtgatc agggacacga tggatcttaa acacttgtat 540caagtggcgg ctgtggctgt gttgtgtgtt caaccggaac cgggataccg gccgttgata 600accgacgtct tgcattctct ggttcctctc gtgccggttg aactcggagg gactctacga 660gttgcggaaa caggttgcgg cacagttgac ttatga 69687231PRTCENTAUREA MACULOSA 87Cys Ala His Asp Glu Thr Lys Leu Leu Val Tyr Glu Leu Met His Asn1 5 10 15Gly Ser Leu Glu Thr Gln Leu His Gly Pro Ser Cys Gly Ser Asn Leu 20 25 30Thr Trp His Cys Arg Met Lys Ile Ala Leu Asp Ile Ala Arg Gly Leu 35 40 45Glu Tyr Leu His Glu His Cys Lys Pro Ser Val Ile His Arg Asp Leu 50 55 60Lys Ser Ser Asn Ile Leu Leu Asp Ser Lys Phe Asn Ala Lys Leu Ser65 70 75 80Asp Phe Gly Leu Ala Val Met Asn Gly Ala Asn Thr Lys Asn Ile Lys 85 90 95Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asn Gly 100 105 110Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu 115 120 125Glu Leu Leu Leu Lys Arg Arg Pro Val Glu Lys Leu Ala Pro Ser Glu 130 135 140Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Thr145 150 155 160Lys Leu Pro Ser Val Ile Asp Pro Val Ile Arg Asp Thr Met Asp Leu 165 170 175Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro 180 185 190Glu Pro Gly Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu Val 195 200 205Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Val Ala Glu Thr 210 215 220Gly Cys Gly Thr Val Asp Leu225 23088842DNACICHORIUM INTYBUS 88tggatttgga tgcgtttaaa agctcaactc aatgataact tattagttgc ggtcaaacga 60ctagacaata aaagtcaaaa ttccatcaaa gaattccaga cggaagtgaa tattttgagt 120aaaattcaac atccaaatat aattagtttg ttgggatatt gcgatcatga tgaaagcaag 180ctacttgttt acgaattgat gcaaaatggt tctttagaaa ctcagttaca tgggccttct 240tgtggatcca atttaacatg gtattgccgg atgaaaattg ccctagatat agcaagagga 300ttggaatatt tacatgaaca ctccaaacca tctgtgattc atagagatct caaatcatct 360aatatacttc ttgattcaaa tttcaatgca aagctttcgg attttggtct tgcggtgatg 420gaaggtgcaa atagcaaaaa cattaaactt tcggggacat tgggatacgt agcacccgaa 480tatcttttag atgggaaatt aaccgataaa agtgacgtgt atgcatttgg agtcgtactt 540tttgagcttt tactcagaag acgacacgtt gaaaaactag aatcatcaca atcccgccaa 600tctattgtca cttgggcgat gccactacta atggacagat cgaagcttcc gagtgtgata 660gatcctgtga ttagggatac aatggatctt aaacatcttt atcaagtggc tgcggtggcg 720gtgttgtgtg ttcaatcgga accgagttac cgtccgttga taaccgatgt tttacattct 780cttgttcctc ttgtcccggt tgaacttgga gggacactta gagttgtaga aaagagtgtt 840gt 84289281PRTCICHORIUM INTYBUS 89Trp Ile Trp Met Arg Leu Lys Ala Gln Leu Asn Asp Asn Leu Leu Val1 5 10 15Ala Val Lys Arg Leu Asp Asn Lys Ser Gln Asn Ser Ile Lys Glu Phe 20 25 30Gln Thr Glu Val Asn Ile Leu Ser Lys Ile Gln His Pro Asn Ile Ile 35 40 45Ser Leu Leu Gly Tyr Cys Asp His Asp Glu Ser Lys Leu Leu Val Tyr 50 55 60Glu Leu Met Gln Asn Gly Ser Leu Glu Thr Gln Leu His Gly Pro Ser65 70 75 80Cys Gly Ser Asn Leu Thr Trp Tyr Cys Arg Met Lys Ile Ala Leu Asp 85 90 95Ile Ala Arg Gly Leu Glu Tyr Leu His Glu His Ser Lys Pro Ser Val 100 105 110Ile His Arg Asp Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Asn Phe 115 120 125Asn Ala Lys Leu Ser Asp Phe Gly Leu Ala Val Met Glu Gly Ala Asn 130 135 140Ser Lys Asn Ile Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu145 150 155 160Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe 165 170 175Gly Val Val Leu Phe Glu Leu Leu Leu Arg Arg Arg His Val Glu Lys 180 185 190Leu Glu Ser Ser Gln Ser Arg Gln Ser Ile Val Thr Trp Ala Met Pro 195 200 205Leu Leu Met Asp Arg Ser Lys Leu Pro Ser Val Ile Asp Pro Val Ile 210 215 220Arg Asp Thr Met Asp Leu Lys His Leu Tyr Gln Val Ala Ala Val Ala225 230 235 240Val Leu Cys Val Gln Ser Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp 245 250 255Val Leu His Ser Leu Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr 260 265 270Leu Arg Val Val Glu Lys Ser Val Val 275 28090495DNACUCUMIS MELO 90attcttttag atgcaaactt caatgccaag ctttctgatt ttggcttgtc tgtcattgtt 60ggagcacaaa acaagaatga tataaagctt tccggaacga tgggttatgt tgctcctgaa 120tatcttttag atggtaaatt gactgataaa agtgatgtct atgcttttgg agttgtgctt 180ttggagcttc ttttaggaag aaggcctgtt gaaaaactgg caccatctca atgtcaatcc 240attgtcacat gggctatgcc tcaactcact gatagatcaa agttacccga tatcgttgat 300ccggtgatca gacacacaat ggaccctaaa catttatttc aggttgctgc tgtcgccgtg 360ctgtgtgtgc aaccagaacc gagctatcgt cccctaataa cagatctttt gcactctctt 420attcctcttg ttcctgttga gctaggaggt actcacagat catcaacatc acaagctcct 480gtggctccag cttag 49591164PRTCUCUMIS MELO 91Ile Leu Leu Asp Ala Asn Phe Asn Ala Lys Leu Ser Asp Phe Gly Leu1 5 10 15Ser Val Ile Val Gly Ala Gln Asn Lys Asn Asp Ile Lys Leu Ser Gly 20 25 30Thr Met Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr 35 40 45Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu 50 55 60Leu Gly Arg Arg Pro Val Glu Lys Leu Ala Pro Ser Gln Cys Gln Ser65 70 75 80Ile Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro 85 90 95Asp Ile Val Asp Pro Val Ile Arg His Thr Met Asp Pro Lys His Leu 100 105 110Phe Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser 115 120 125Tyr Arg Pro Leu Ile Thr Asp Leu Leu His Ser Leu Ile Pro Leu Val 130 135 140Pro Val Glu Leu Gly Gly Thr His Arg Ser Ser Thr Ser Gln Ala Pro145 150 155 160Val Ala Pro Ala92375DNAERAGROSTIS CURVULA 92gatgggaagc tcaccgagaa aagcgacgtg tacgcgtttg gcatagtgct tcttgagctg 60ctaatgggaa ggaagcctgt tgagaagttg agtcaatctc agtgccaatc aattgtgact 120tgggccatgc cccaactgac agacagatca aaacttccca acataattga cccagtgatc 180agggacacaa tggatccaaa gcacttgtat caggttgcag cagtggctgt tctatgcgtg 240caaccagaac cgagttacag accactgata acggatgttc tccactcttt agttcctcta 300gtgcctgtgg agcttggtgg gacactaagg gttgcagagc caccgtcccc aaaccaaaat 360cattctcctc gttga 37593124PRTERAGROSTIS CURVULA 93Asp Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly Ile Val1 5 10 15Leu Leu Glu Leu Leu Met Gly Arg Lys Pro Val Glu Lys Leu Ser Gln 20 25 30Ser Gln Cys Gln Ser Ile Val Thr Trp Ala Met Pro Gln Leu Thr Asp 35 40 45Arg Ser Lys Leu Pro Asn Ile Ile Asp Pro Val Ile Arg Asp Thr Met 50 55 60Asp Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val65 70 75 80Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser 85 90 95Leu Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Val Ala 100 105 110Glu Pro Pro Ser Pro Asn Gln Asn His Ser Pro Arg 115 12094414DNAGERBERA HYBRID 94ggggttcatg gcaagaacaa tataaaactt tcaggaactt taggatatgt cgcgccggaa 60taccttttag atggtaaact tactgataaa agtgacgttt atgcgtttgg agttgtgctt 120ctcgagcttt tgataggacg aaaacccgtg gagaaaatgt caccatttca atgccaattt 180atcgttacat gggcaatgcc tcagctaacg gacagatcga agcttcctaa tcttgtggat 240cctgtgatta gagatactat ggacttgaag cccttatatc aagttgcggc tgtaactgtg 300ttatgtgtac aacccgaacc aagttaccgc ccattaataa cggatgtttt gcattcgttc 360atcccacttg tacctgctga tcttggaggg tcgttaaaag ttgtcgactt ttaa 41495137PRTGERBERA HYBRID 95Gly Val His Gly Lys Asn Asn Ile Lys Leu Ser Gly Thr Leu Gly Tyr1 5 10 15Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys Ser Asp 20 25 30Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu Ile Gly Arg Lys 35 40 45Pro Val Glu Lys Met Ser Pro Phe Gln Cys Gln Phe Ile Val Thr Trp 50 55 60Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Leu Val Asp65 70 75 80Pro Val Ile Arg Asp Thr Met Asp Leu Lys Pro Leu Tyr Gln Val Ala 85 90 95Ala Val Thr Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu 100 105 110Ile Thr Asp Val Leu His Ser Phe Ile Pro Leu Val Pro Ala Asp Leu 115 120 125Gly Gly Ser Leu Lys Val Val Asp Phe 130 13596498DNAHELIANTHUS PARADOXUS 96atcgtgttcc attttggttg ttgtctaaag ctttcagatt ttggtcttgc tgtaatggat 60ggagcccaga acaaaaacaa catcaagctt tcagggacat tgggttatgt agctccagag 120tatcttttag atggaaaact gaccgacaaa agtgatgtat atgcatttgg agttgtactt 180ttagagcttc tacttggaag acggcctgta gaaaaactgg ccgcatctca atgccaatct 240atcgtcactt gggccatgcc acagctaaca gacagatcaa agctcccaaa tattgtcgat 300cctgtaatca gatatacgat ggatctcaaa cacttgtacc aagttgctgc cgtggcagtg 360ctgtgtgtgc aaccagagcc aagttaccgg ccattaataa ccgatgtttt gcattctctt 420atccctcttg ttccggtgga gctcggggga actctaaaag ctccacaaac aaggtcttcg 480gtaacaaatg acccgtga 49897165PRTHELIANTHUS PARADOXUS 97Ile Val Phe His Phe Gly Cys Cys Leu Lys Leu Ser Asp Phe Gly Leu1 5 10 15Ala Val Met Asp Gly Ala Gln Asn Lys Asn Asn Ile Lys Leu Ser Gly 20 25 30Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp Gly Lys Leu Thr 35 40 45Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu Leu Glu Leu Leu 50 55 60Leu Gly Arg Arg Pro Val Glu Lys Leu Ala Ala Ser Gln Cys Gln Ser65 70 75 80Ile Val Thr Trp Ala Met Pro Gln Leu Thr Asp Arg Ser Lys Leu Pro 85 90 95Asn Ile Val Asp Pro Val Ile Arg Tyr Thr Met Asp Leu Lys His Leu 100 105 110Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln Pro Glu Pro Ser 115 120 125Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu Ile Pro Leu Val 130 135 140Pro Val Glu Leu Gly Gly Thr Leu Lys Ala Pro Gln Thr Arg Ser Ser145 150 155 160Val Thr Asn Asp Pro 16598612DNAIPOMOEA NIL 98cgtggatcaa ctttaagttg gcctctccga atgaaaattg ctttggatat tgcaagagga 60ttagaatacc ttcacgagcg ttgcaacccc cctgtgatcc ataggcatct caaatcgtct 120aatattcttc ttgattccag cttcaacgca aagatttctg attttggcct ttctgtaact 180ggcggaaacc taagcaagaa cataaccaag atttcgggat cactgggtta tcttgctcca 240gagtatctct tagacggtaa actaactgat aagagtgatg tgtatggttt tggcattatt 300cttctagagc ttttgatggg taaaaggcca gtggagaaag tgggagaaac taagtgccaa 360tcaatagtta catgggctat gccccagctt acggaccgat caaagcttcc gaatattgtt 420gaccctacga tcaggaacac aatggatgtt aagcatttat atcaggttgc ggctgtagct 480gtgttatgtg tgcaaccgga gccaagctat aggccattga taactgatgt actacactcc 540ttcattccac ttgtaccaaa tgaactcggg gggtcgctta gggtagtgga ttctactccc 600cattgctcat ag 61299203PRTIPOMOEA NIL 99Arg Gly Ser Thr Leu Ser Trp Pro Leu Arg Met Lys Ile Ala Leu Asp1 5 10 15Ile Ala Arg Gly Leu Glu Tyr Leu His Glu Arg Cys Asn Pro Pro Val 20 25 30Ile His Arg His Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe 35 40 45Asn Ala Lys Ile Ser Asp Phe Gly Leu Ser Val Thr Gly Gly Asn Leu 50 55 60Ser Lys Asn Ile Thr Lys Ile Ser Gly Ser Leu Gly Tyr Leu Ala Pro65 70 75 80Glu Tyr Leu Leu Asp Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Gly 85 90

95Phe Gly Ile Ile Leu Leu Glu Leu Leu Met Gly Lys Arg Pro Val Glu 100 105 110Lys Val Gly Glu Thr Lys Cys Gln Ser Ile Val Thr Trp Ala Met Pro 115 120 125Gln Leu Thr Asp Arg Ser Lys Leu Pro Asn Ile Val Asp Pro Thr Ile 130 135 140Arg Asn Thr Met Asp Val Lys His Leu Tyr Gln Val Ala Ala Val Ala145 150 155 160Val Leu Cys Val Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp 165 170 175Val Leu His Ser Phe Ile Pro Leu Val Pro Asn Glu Leu Gly Gly Ser 180 185 190Leu Arg Val Val Asp Ser Thr Pro His Cys Ser 195 200100708DNANUPHAR ADVENA 100ttagataatg gcggacccga ttgtcaacga gaattcgaga atgaggttga tttgatgagt 60agaattaggc atccaaatgt ggtttcttta ttgggttatt gcattcatgg agaaaccagg 120cttcttgtct atgaaatgat gcaaaacggg acgttggaat cgctattgca tggaccatca 180catggatcct cactaacttg gcacattcgt atgaagatcg ccctcgacac agcaagaggc 240ctcgagtatc tgcatgaaca ctgcgacccc tctgtgatcc accgtgacct gaagccttct 300aacattcttt tggattccaa ctacaattcc aagctctcag actttggtct tgcagtcact 360gttggaagcc agaatcaaac caacattaag attctaggga cactgggtta ccttgcacca 420gagtacgttt tgaatggcaa attgacagag aaaagtgatg tgtttgcttt tggagttgtc 480ctgttggagc ttctcatggg caagaaacca gtggagaaga tggcatcccc tccatgccaa 540tccattgtca catgggcgat gcctcatctt actgacagaa ttaagcttcc aaatatcatt 600gatcctgtta ttagaaacac catggatctg aaacacttgt accaggttgc agctgttgct 660gttctctgcg tacaaccaga gccccagtta tcgtcctctg ataactga 708101235PRTNUPHAR ADVENA 101Leu Asp Asn Gly Gly Pro Asp Cys Gln Arg Glu Phe Glu Asn Glu Val1 5 10 15Asp Leu Met Ser Arg Ile Arg His Pro Asn Val Val Ser Leu Leu Gly 20 25 30Tyr Cys Ile His Gly Glu Thr Arg Leu Leu Val Tyr Glu Met Met Gln 35 40 45Asn Gly Thr Leu Glu Ser Leu Leu His Gly Pro Ser His Gly Ser Ser 50 55 60Leu Thr Trp His Ile Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly65 70 75 80Leu Glu Tyr Leu His Glu His Cys Asp Pro Ser Val Ile His Arg Asp 85 90 95Leu Lys Pro Ser Asn Ile Leu Leu Asp Ser Asn Tyr Asn Ser Lys Leu 100 105 110Ser Asp Phe Gly Leu Ala Val Thr Val Gly Ser Gln Asn Gln Thr Asn 115 120 125Ile Lys Ile Leu Gly Thr Leu Gly Tyr Leu Ala Pro Glu Tyr Val Leu 130 135 140Asn Gly Lys Leu Thr Glu Lys Ser Asp Val Phe Ala Phe Gly Val Val145 150 155 160Leu Leu Glu Leu Leu Met Gly Lys Lys Pro Val Glu Lys Met Ala Ser 165 170 175Pro Pro Cys Gln Ser Ile Val Thr Trp Ala Met Pro His Leu Thr Asp 180 185 190Arg Ile Lys Leu Pro Asn Ile Ile Asp Pro Val Ile Arg Asn Thr Met 195 200 205Asp Leu Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val 210 215 220Gln Pro Glu Pro Gln Leu Ser Ser Ser Asp Asn225 230 23510230DNAARTIFICIAL SEQUENCESynthetic Primer 102tcggctcggc ccagaacaag atcgcaagac 3010334DNAARTIFICIAL SEQUENCESynthetic Primer 103ctacattctc tcctcgtatt attcctcgtt gact 3410432DNAARTIFICIAL SEQUENCESynthetic Primer 104actttcagat gagtggatca taaccctata ca 3210531DNAARTIFICIAL SEQUENCESynthetic Primer 105agatacaatg gatctcaaac acttatacca g 3110642DNAARTIFICIAL SEQUENCESynthetic Primer 106aaaggatcca tgggaagtgg tgaagaagat agatttgatg ct 4210740DNAARTIFICIAL SEQUENCESynthetic Primer 107tttctgcagt ctgtgaatca tcttgttaac cggagagtcc 4010830DNAARTIFICIAL SEQUENCESynthetic Primer 108tctgagtttt aatcgagcca agtcgtctca 3010953DNAARTIFICIAL SEQUENCESynthetic Primer 109tatcccggga aaatgagaga gcttcttctt cttcttcttc ttcattttca gtc 5311038DNAARTIFICIAL SEQUENCESynthetic Primer 110tttggatcct gtgaatcatc ttgttaaccg gagagtcc 3811141DNAARTIFICIAL SEQUENCESynthetic Primer 111atacccgggt ctgtgtcagg aatccaaatg ggaagtggtg a 4111241DNAARTIFICIAL SEQUENCESynthetic Primer 112aaaggatcct ctgtgtcagg aatccaaatg ggaagtggtg a 4111339DNAARTIFICIAL SEQUENCESynthetic Primer 113aaatctagac tgtgaatcat cttgttaacc ggagagtcc 3911435DNAARTIFICIAL SEQUENCESynthetic Primer 114atagagctcg caagaaccaa tctccaaaat ccatc 3511537DNAARTIFICIAL SEQUENCESynthetic Primer 115atagagctcg agggtcttga tatcgaaaaa ttgcacg 3711637DNAARTIFICIAL SEQUENCESynthetic Primer 116ataggatcct cgcaagaacc aatctccaaa atccatc 3711740DNAARTIFICIAL SEQUENCESynthetic Primer 117atatctagac tcgagggtct tgatatcgaa aaattgcacg 4011852DNAARTIFICIAL SEQUENCESynthetic Primer 118atatctagaa aatgagagag cttcttcttc ttcttcttct tcattttcag tc 5211947DNAARTIFICIAL SEQUENCESynthetic Primer 119ataggatcct gttaaaagcg atttataatt tacaccgttt tggtgta 4712041DNAARTIFICIAL SEQUENCESynthetic Primer 120atacccggga aaagtttttg atgaaattca atctaaagac t 411211309DNAARABIDOPSIS THALIANA 121aaaatgagag agcttcttct tcttcttctt cttcattttc agtctctaat tcttttgatg 60atcttcatca ctgtctctgc ttcttctgct tcaaatcctt ctttagctcc tgtttactct 120tccatggcta cattctctcc tcgaatccaa atgggaagtg gtgaagaaga tagatttgat 180gctcataaga aacttctgat tggtctcata atcagtttct cttctcttgg ccttataatc 240ttgttctgtt ttggcttttg ggtttatcgc aagaaccaat ctccaaaatc catcaacaac 300tcagattctg agagtgggaa ttcattttcc ttgttaatga gacgacttgg ctcgattaaa 360actcagagaa gaacttctat ccaaaagggt tacgtgcaat ttttcgatat caagaccctc 420gagaaagcga caggcggttt taaagaaagt agtgtaatcg gacaaggcgg tttcggatgc 480gtttacaagg gttgtttgga caataacgtt aaagcagcgg tcaagaagat cgagaacgtt 540agccaagaag caaaacgaga atttcagaat gaagttgact tgttgagcaa gatccatcac 600tcgaacgtta tatcattgtt gggctctgca agcgaaatca actcgagttt catcgtttat 660gagcttatgg agaaaggatc attagatgaa cagttacatg ggccttctcg tggatcagct 720ctaacatggc acatgcgtat gaagattgct cttgatacag ctagaggact agagtatctc 780catgagcatt gtcgtccacc agttatccac agagatttga aatcttcgaa tattcttctt 840gattcttcct tcaacgccaa gatttcagat ttcggttttg ctgtatcgct ggatgaacat 900ggcaagaaca acattaaact ctctgggaca cttggttatg ttgccccgga atacctcctt 960gacggaaaac tgacggataa gagtgatgtt tatgcatttg gggtagttct gcttgaactc 1020ttgttgggta gacgaccagt tgaaaaatta actccagctc aatgccaatc tcttgtaact 1080tgggcaatgc cacaacttac cgatagatcc aagcttccaa acattgtgga tgccgttata 1140aaagatacaa tggatctcaa acacttatac caggtagcag ccatggctgt gttgtgcgtg 1200cagccagaac caagttaccg gccgttgata accgatgttc ttcactcact tgttccactg 1260gttccggtag agctaggagg gactctccgg ttaacaagat gattcacag 130912225DNAARTIFICIAL SEQUENCESynthetic Primer 122tcggacaagg cggtttcgga tgcgt 2512332DNAARTIFICIAL SEQUENCESynthetic Primer 123tagtcctcta gctgtatcaa gagcaatctt ca 3212432DNAARTIFICIAL SEQUENCESynthetic Primer 124tatcattgtt gggctctgca agtgaaatca ac 3212532DNAARTIFICIAL SEQUENCESynthetic Primer 125tggagaaagg atccttagat gatcagttac at 3212631DNAARTIFICIAL SEQUENCESynthetic Primer 126tccatgtaac tgatcatcta aggatccttt c 3112737DNAARTIFICIAL SEQUENCESynthetic Primer 127ataaacgacg aaactcgagt tgatttcact tgcagag 3712828DNAARTIFICIAL SEQUENCESynthetic Primer 128aaaatgaaga aactggttca tcttcagt 2812927DNAARTIFICIAL SEQUENCESynthetic Primer 129tagacttcta ttctcacatt cttacac 2713027DNAARTIFICIAL SEQUENCESynthetic Primer 130tccaatgatc cattatgcat cagctca 2713129DNAARTIFICIAL SEQUENCESynthetic Primer 131tcgttctcaa attctctctc agcatgttg 2913229DNAARTIFICIAL SEQUENCESynthetic Primer 132tccggatatg ccaggtcagc gctgatcca 2913328DNAARTIFICIAL SEQUENCESynthetic Primer 133tccagggatc ccttctccat gagctcat 2813441DNAARTIFICIAL SEQUENCESynthetic Primer 134aaagagctct ctgtgtcagg aatccaaatg ggaagtggtg a 4113547DNAARTIFICIAL SEQUENCESynthetic Primer 135atagctagct gttaaaagcg atttataatt tacaccgttt tggtgta 4713643DNAARTIFICIAL SEQUENCESynthetic Primer 136atagctagca gaaaagtttt tgatgaaatt caatctaaag act 4313729DNAARTIFICIAL SEQUENCESynthetic Primer 137tctgggttta tcatcatacc aagtatcca 2913831DNAARTIFICIAL SEQUENCESynthetic Primer 138attcagttcc atcaagattg ttggcatgga c 3113931DNAARTIFICIAL SEQUENCESynthetic Primer 139tggagggagg tggccctgag tgcgagaagg a 3114027DNAARTIFICIAL SEQUENCESynthetic Primer 140gctggatctg cttggcagga ttcggca 2714131DNAARTIFICIAL SEQUENCESynthetic Primer 141atatctagat gctaggttat agatccatgc a 3114233DNAARTIFICIAL SEQUENCESynthetic Primer 142ataggatcca ccagaactat atatacgaag gca 3314340DNAARTIFICIAL SEQUENCESynthetic Primer 143aggacgactt ggctcgatta aaatcacagg tcgtgatatg 4014435DNAARTIFICIAL SEQUENCESynthetic Primer 144taatcgagcc aagtcgtcct acatatatat tccta 3514535DNAARTIFICIAL SEQUENCESynthetic Primer 145taatcgagcc aagtcgtcct ctcttttgta ttcca 3514642DNAARTIFICIAL SEQUENCESynthetic Primer 146aggacgactt ggctcgatta aaatcaaaga gaatcaatga tc 4214721DNAARTIFICIAL SEQUENCESynthesized gene fragment 147gacgacttgg ctcgattaaa a 21148399DNAARABIDOPSIS THALIANA 148tgctaggtta tagatccatg caaatatgga gtagatgtac aaacacacgc tcggacgcat 60attacacatg ttcatacact taatactcgc tgttttgaat tgatgtttta ggaatatata 120tgtagagaga gcttccttga gtccattcac aggtcgtgat atgattcaat tagcttccga 180ctcattcatc caaataccga gtcgccaaaa ttcaaactag actcgttaaa tgaatgaatg 240atgcggtaga caaattggat cattgattct ctttgattgg actgaaggga gctccctctc 300tcttttgtat tccaattttc ttgattaatc tttcctgcac aaaaacatgc ttgatccact 360aagtgacata tatgctgcct tcgtatatat agttctggt 399149399DNAARTIFICIAL SEQUENCEArtificial microRNA construct 149tgctaggtta tagatccatg caaatatgga gtagatgtac aaacacacgc tcggacgcat 60attacacatg ttcatacact taatactcgc tgttttgaat tgatgtttta ggaatatata 120tgtaggacga cttggctcga ttaaaatcac aggtcgtgat atgattcaat tagcttccga 180ctcattcatc caaataccga gtcgccaaaa ttcaaactag actcgttaaa tgaatgaatg 240atgcggtaga caaattggat cattgattct ctttgatttt aatcgagcca agtcgtcctc 300tcttttgtat tccaattttc ttgattaatc tttcctgcac aaaaacatgc ttgatccact 360aagtgacata tatgctgcct tcgtatatat agttctggt 3991501475DNAARABIDOPSIS THALIANA 150cttagccaat ggatgaggat gacacgataa tgataatcaa agatcaacat ggcacgctca 60agaccgcctt tagaagtcct ctctaaattc tttcttccga tctcctaaat atgttttgtt 120ttggtcaaat aaattgatag gtaatactta gtgattatac tatttggttt ttgttttatc 180attgactatt tcacttttat aaatcaaata cttatcaaaa ttgttctttc cgtatgtatt 240catattttct aatattgtaa agatttgttt cacctaacat ctgtacccat ctttgatcat 300tgacaaaata tatattagaa tggccttaga acgtgttagg catcttccta ctattatcat 360attacctaat ccccaatttt attacatttt ttaatttcta aaagagcttg aatataatgt 420catttcgaat atctctgttc atcttttttt ttttctgtgc gacttctgac ccaaagcctt 480cgacgatttt ttccaatctg aaaacttttg aataaggaac ttagtcaatg gtcaacacct 540tgctaattaa acaaagttcc attgatacaa taatgagatt tttgtacatt aacgctttca 600tatagttttt gcgattcaac agataatctt aaaattaagg agtcctattg ataaagtctt 660gttcaaacgt acaaactcaa tccacacaaa accttcataa aatacgatat aggaaataaa 720gattgttttt gcgtgagaaa atactatatg aactcaaaag attttaaaac aatttgtatt 780aatacataaa caattgttgt gatacacccg tgtaaaattt taagattgtt tttttctgaa 840attcttcaag gaaacttata gcttaaaatc tacacttcaa atactctgtt ttaaaggcat 900taaaaataac tgcgtttcag aaaaatattg aaattttagc tgatcttttg ctacaaattt 960aaggaatctt ggcacctgca gaatctataa catgttcatt aagtaatgca atagttatac 1020aattatacat tatttgcatc atacttatat tatagtgata ttaacaaacc catgttctca 1080gcacactttt acgtagaaaa acataaaaac ccaaatagga agaagccact cataaggata 1140atgggtttat ataattcaca gcaaagaaag ccatcgaact attcgattaa ttatccattc 1200tttttttttt tagtttgaat gtataagaac aaagagttgt tacgcatcat gacaatgtct 1260tagaaaacaa aagaaatgaa taaaaaagta aaacgaaaaa taaaaagtga ggatgaagtt 1320gttgaatgag ttggcgaggc ggcgactttt tcatacattc catttactta attcctaaag 1380tccttctcac atctctttgt tatataatga caccataacc atttcttctc ttcacaatct 1440ttacaagaat atctctcttc tacagtaaac aaaaa 147515131DNAARTIFICIAL SEQUENCESynthetic Primer 151acgtaagctt cttagccaat ggatgaggat g 3115231DNAARTIFICIAL SEQUENCESynthetic Primer 152acgttctaga tttttgttta ctgtagaaga g 31153338DNABRASSICA NAPUS 153tgctgcttca aatccttcta tagctcctgt ttataccacc atgactactt tctctccagg 60aattcaaatg ggaagtggtg aagaacacag attagatgca cataagaaac tcctgattgg 120tcttataatc agttcctctt ctcttggtat cgtaatcttg atttgctttg gcttctggat 180gtactgtcgc aagaaagctc ccaaacccat caagattccg gatgctgaga gtgggacttc 240atcattttca atgtttgtga ggcggctaag ctcaatcaaa actcagagaa catctagcaa 300tcagggttat gtgcagcgtt tcgattccaa gacgctag 33815438DNAARTIFICIAL SEQUENCESynthetic Primer 154tatggatcct gctgcttcaa atccttctat agctcctg 3815537DNAARTIFICIAL SEQUENCESynthetic Primer 155tattctagac tagcgtcttg gaatcgaaac gctgcac 3715638DNAARTIFICIAL SEQUENCESynthetic Primer 156tatgagctct gctgcttcaa atccttctat agctcctg 3815737DNAARTIFICIAL SEQUENCESynthetic Primer 157tatgagctcc tagcgtcttg gaatcgaaac gctgcac 3715824DNAARTIFICIAL SEQUENCESynthetic Primer 158gcagatcgct cctcccgtcg tgat 2415928DNAARTIFICIAL SEQUENCESynthetic Primer 159cgcctaggag cgacgggtac tcgatcat 2816028DNAARTIFICIAL SEQUENCESynthetic Primer 160cctagctaag cgacgggtac tcgatcat 28161272DNABRACHYPODIUM DISTACHYON 161gctcctcccg tcgtgatcac agtggtgagg caccaccatt accaccggga gctggtcatc 60tccgctgtcc tcgcctgcgt cgccaccgcc atgatcctcc tctccacact ctacgcctgg 120acgatgtggc ggcggtctcg ccggaccccc cacggcggca agggccgcgg ccggagatca 180gggatcacac tggtgccaat cctgagcaag ttcaattcag tgaagatgag caggaagggg 240ggccttgtga cgatgatcga gtacccgtcg ct 27216231DNAARTIFICIAL SEQUENCESynthetic Primer 162cgggatcccg gcataacaaa ctcgtgcatc c 3116330DNAARTIFICIAL SEQUENCESynthetic Primer 163ccatcgatgg cgccaaacac aatagctcaa 301641174DNABRACHYPODIUM DISTACHYON 164gtaagtaatt tcaagtttaa gtttcataag cataacaaac tcgtgcatcc aatttgaacc 60attttactgt cctggcatcc tctaaatatt tccttgatta tcagcttatc ttcatcccat 120tgaatcagaa aattaccaac ccttgtttta gctttaatca ttgttatttg ttgtctgagg 180ggctacactg tttctttata ttggtgaagg agttaccagg caaaaattcc cacctcctga 240tattagcaga gacccccttt tttgtgcctg tatgcatact aacaaataat acagatggaa 300atatgtatat ttgttatatc atggattgat gctttatgtt tagcaagtcc atgcaatggt 360agtcaaaaga tgtaaacttt tgaatgatat attggggctt tagattagcc atttttaccc 420tcacttgaaa atgacaattt tgcccttccg atctactttc tcttgtcacc tcaggcaggc 480tcttgaaagt tcttatccct gaattccgtg gaagtttatt attctaatgt tatagtttac 540ttaaagtgtc gcataatcta ctagagccta atggaagtac tgatggactt tgttttgcta 600caatcactgc ttgcaagaat gactactttg gggcatttct aatatattat tgatatttct 660atgatgtatt gttgtccatg tacttcagtc cttacagcga ctagtcctat ttctgcattg 720ataaattgtt cactgtcaga ccatcttgag tggcaagaat gagtataaca tgtcttgttt 780ttctgtgatt tcaaggtaag cgcacatgcg cacagtgtac accgtcacca catgtgagta 840caccccctag tacacatgta aaaaaagcac agtccagtta ttaaatggac cattggcatt 900gattgtcgtg tttataggag taaagataca tgtaaacact aattcattgg gagatataaa 960tttatactac cattgaatgt gacataggct ctaaggtttt tagttcagca tttcgaaaga 1020gctttgtttg gttggcttgg gatggaatca ggtgacaaca tttttgggtt gcagcaaatt 1080taatattgat tgaggaggca tacaacgaaa tcattgagct attgtgtttg gcgttacatc 1140tatggaattt cttctaatct gattattgtt tgta 117416522DNAARTIFICIAL SEQUENCESynthetic Primer 165gatccgctcc tcccgtcgtg at 2216630DNAARTIFICIAL SEQUENCESynthetic Primer 166aacgcgatcg cttgcatgcc tgcagtagac 3016728DNAARTIFICIAL SEQUENCESynthetic Primer 167gacttaatta agaattcgag ctcgggta 28168251DNAPANICUM VIRGATUM 168tcgtagtgca ccaccatttc caccgcgagc tggtcatcgc cgccgtcctc gcctgcatcg 60ccaccgtcac gatcttcctt tccacgctct acgcttggac actatggcgg cgatctcgcc 120ggagcaccgg cggcaaggtc accaggagct cagacgcagc gaaggggatc aagctggtgc 180cgatcttgag caggttcaac tcggtgaaga tgagcaggaa gaggctggtt gggatgttcg 240agtacccgtc g 25116927DNAARTIFICIAL SEQUENCESynthetic Primer 169gcagatctcg tagtgcacca ccatttc 2717027DNAARTIFICIAL SEQUENCESynthetic Primer 170cgcctaggcg acgggtactc gaacatc

2717127DNAARTIFICIAL SEQUENCESynthetic Primer 171cctagctacg acgggtactc gaacatc 2717225DNAARTIFICIAL SEQUENCESynthetic Primer 172gatcctcgta gtgcaccacc atttc 2517321DNAARTIFICIAL SEQUENCESynthetic Primer 173ctcgtagtgc accaccattt c 21174261DNASORGHUM BICOLOR 174aatgggaccg cctccgttgc tccggcggtg ccggcgccgc ctcccgtcgt gatcatcgtg 60gagcggcgcc atcatttcca ccgcgagcta gtcatcgcct ccgttctcgc ctccatcgcc 120atcgtcgcga ttatcctctc cacgctctat gcgtggatcc tgtggcggcg gtctcgccgg 180ctgcccagcg gcaagggcgc caggagcgca gacaccgcga ggggaatcat gctggtgccg 240atcctgagca agttccactc a 26117526DNAARTIFICIAL SEQUENCESynthetic Primer 175gcagatcaat gggaccgcct ccgttg 2617628DNAARTIFICIAL SEQUENCESynthetic Primer 176cgcctaggtg agtggaactt gctcagga 2817728DNAARTIFICIAL SEQUENCESynthetic Primer 177cctagctatg agtggaactt gctcagga 28178273DNASORGHUM BICOLOR 178gtaagtattc ttgcaacaca ttactatttt caataaccac aagtttaaaa gcttgagtcc 60atttcgcaaa ccagttgttc ataaccaaat tcttaggtaa ttaggtccaa ttgagaaaat 120ctgatcattg aacactagca ggaaataact cagacatagt ttctgcatac tataatgatg 180cttaatatat ttgttctctt ttgagattgt attgcataga catttctgtg taaaataatg 240ttttacatca tgtatatata tcacttttta tag 27317930DNAARTIFICIAL SEQUENCESynthetic Primer 179cgggatcctt cttgcaacac attactattt 3018034DNAARTIFICIAL SEQUENCESynthetic Primer 180ccatcgatga aatgtctatg caatacaatc tcaa 3418124DNAARTIFICIAL SEQUENCESynthetic Primer 181gatccaatgg gaccgcctcc gttg 2418221DNAARTIFICIAL SEQUENCESynthetic Primer 182caatgggacc gcctccgttg a 211831000DNASORGHUM BICOLOR 183ggccccggcc gcgcgcgtct ccgtgtcctc cgcgactgtg cacgtttcgt cgggagcggc 60gtgcccacgc ccaccccccg tccaccagcc agcaaccgac ggcactggtg acacgcggct 120ggtccgctcg gtccgccccg cggctccaga tcacggcaag cgcgcccgcc gcccgctgct 180gcgctgcgct gcacgtcccg ccctgacgcc acgccacgcc aagcgcgaca cgacacgaca 240cgacacgacc cgacccccgc caacgaaacg ccgaaacgcg gcaacgcgtg acgggcgcgc 300atggtcgatg ctctacccgc gcgtccgccc cacgccaatc tcccggcggg tccctcgtgg 360gacggggaac gcgatgcggc tgcaggctgc gaccgcgacc gcgaccgcga ccgcgcccac 420gtgaaggcag gcaggcagcc ccggagcggg cgcggcggtg ggccaacgac gcgttgccgt 480cgcgaatctt cttctggcca cggccaaggg ccaatcgccc gctccgctcc gctccgcact 540ccgcctccgc tagggaatat ggaacccgat cccacggccc tctgggtctg gtcgacgggt 600cctctcgccg tggcagctgc ttcccggacc ggaggatcgc tgagcgcgga cgccactgcc 660attgccgtcc gactatagtt gttaattacc ataaaataat ttgttaacga taaaacccgt 720gtcaggcacc gtcgtctgga cgctgctatg ggataaccat tcgcgtacgt cggttgtatg 780ggtgggatcc tctgcggcac gccattctgg tgctgctagt ggaatagaca aaaaaagggc 840cgacggtgtt tgctcgtggc aggccacaca gagtgacaac cagagtggtt gccgcaaaaa 900caaccaatca cacaaaaagt gttgtaccgg tggaggacag ccattaatca gcaggccggc 960ttcgcggcca aaagaaacgg agaagaggaa aaaggggggc 100018428DNAARTIFICIAL SEQUENCESynthetic Primer 184tcccaagctt gcgcgtctcc gtgtcctc 2818530DNAARTIFICIAL SEQUENCESynthetic Primer 185agtaaagctt cccccttttt cctcttctcc 301861000DNASORGHUM BICOLOR 186taatggtcga gtgaggcccg tatagatgta gttaaatagc taaaattttt ggagaaataa 60gcattttttt ggaagaatat atttaaacat gggcttgtaa aacttggctg taaagatttg 120gaatttagga tcttggagcc ccaaaactgt ataaacttgc ttagggaccc gtgtcttgtg 180tgttgcagac caaaaaattt agaaagcatc taaacaccta tttgaatgta aagtttacag 240ccaaaagttt taggatgtaa agatttggga tctaaaagta gtcattagga aataacacgt 300tagagagaga gagtagatct tcttattggt ttctcatgca ctaatcgaac caatcactgg 360accacttgaa ccaaacttta tcacattgaa ctttgtcagt tcagttcgaa cgcaggactg 420gagctgccct taaggccaat tgctcaagat tcattcaaca attgaaacat ctcccatgat 480taaatcagta taaggttgct atggtcttgc ttgacaaagt tttttttttg agggaatttc 540aactaaattt ttgagtgaaa ctatcaaata ctgattttaa aaatttttta taaaaggaag 600cgcagagata aaaggccatc tatgctacaa aagtacccaa aaatgtaatc ctaaagtatg 660aattgcattt tttttgtttg gacgaaagga aaggagtatt accacaagaa tgatatcatc 720ttcatattta gatctttttt gggtaaagct tgagattctc taaatataga gaaatcagaa 780gaaaaaaaaa ccgtgttttg gtggttttga tttctagcct ccacaataac tttgacggcg 840tcgacaagtc taacggacac caagcagcga accaccagcg ccgagccaag cgaagcagac 900ggccgagacg ttgacacctt cggcgcggca tctctcgaga gttccgctcc ggcgctccac 960ctccaccgct ggcggtttct tattccgttc cgttccgcct 100018726DNAARTIFICIAL SEQUENCESynthetic Primer 187aactgcaggg tcgagtgagg cccgta 2618828DNAARTIFICIAL SEQUENCESynthetic Primer 188ttctgcaggg aacggaacgg aataagaa 28189239DNASORGHUM BICOLOR 189gccgtgggtc gtttaagctg ccgctgtacc tgtgtcgtct ggtgccttct ggtgtacctg 60ggaggttgtc gtctatcaag tatctgtggt tggtgtcatg agtcagtgag tcccaatact 120gttcgtgtcc tgtgtgcatt atacccaaaa ctgttatggg caaatcatga ataagcttga 180tgttcgaact taaaagtctc tgctcaatat ggtattatgg ttgtttttgt tcgtctcct 23919027DNAARTIFICIAL SEQUENCESynthetic Primer 190taggtaccgc cgtgggtcgt ttaagct 2719127DNAARTIFICIAL SEQUENCESynthetic Primer 191aaggtaccag gagacgaaca aaaacaa 2719234DNAARTIFICIAL SEQUENCESynthetic Primer 192aacgcgatcg taatggtcga gtgaggcccg tata 341931302DNABRASSICA NAPUS 193atgaagaaac tggttcatct tcagtttctg tttcttgtca agatctttgc tactcaattc 60ctcactcctt cttcatcatc ttttgctgct tcaaatcctt ctatagctcc tgtttatacc 120accatgacta ctttctctcc aggaattcaa atgggaagtg gtgaagaaca cagattagat 180gcacataaga aactcctgat tggtcttata atcagttcct cttctcttgg tatcgtaatc 240ttgatttgct ttggcttctg gatgtactgt cgcaagaaag ctcccaaacc catcaagatt 300ccggatgctg agagtgggac ttcatcattt tcaatgtttg tgaggcggct aagctcaatc 360aaaactcaga gaacatctag caatcagggt tatgtgcagc gtttcgattc caagacgcta 420gagaaagcga caggcggttt caaagacagt aatgtaatcg gacagggcgg tttcggatgc 480gtttacaagg cttctttgga cagcaacact aaagcagcgg ttaaaaagat cgaaaacgtt 540agccaagaag caaaacgaga atttcagaat gaagttgagc tgttgagcaa gatccagcac 600tccaatatta tatcattgtt gggctctgca agtgaaatca actcgagttt cgtcgtttat 660gagttgatgg agaaaggatc cttagatgat cagttacatg gaccttcgtg tggatccgct 720ctaacatggc atatgcgtat gaagattgct ctagatacag ctagaggatt agagtatctc 780catgaacatt gtcgtccacc agttatccac agggacctga aatcgtctaa tatacttctt 840gattcttcct tcaatgccaa gatttcagat tttggtctgg ctgtatcggt tggagtgcat 900gggagtaaca acattaaact ctctgggaca cttggttatg ttgccccgga atatctccta 960gacggaaagt tgacggataa gagtgatgtc tatgcatttg gggtggttct tcttgaactt 1020ttgttgggta gaaggccggt tgagaaattg agtccatctc agtgtcaatc tcttgtgact 1080tgggcaatgc cacaacttac cgatagatcg aaactcccaa acatcgtgga tccggttata 1140aaagatacaa tggatcttaa gcacttatac caggtagcag ccatggctgt gttgtgcgtt 1200cagccagaac cgagttaccg gccgctgata accgatgttc ttcactcact tgttccattg 1260gttccggtcg aactaggagg gactctccgg ttaacccgat ga 1302194433PRTBRASSICA NAPUS 194Met Lys Lys Leu Val His Leu Gln Phe Leu Phe Leu Val Lys Ile Phe1 5 10 15Ala Thr Gln Phe Leu Thr Pro Ser Ser Ser Ser Phe Ala Ala Ser Asn 20 25 30Pro Ser Ile Ala Pro Val Tyr Thr Thr Met Thr Thr Phe Ser Pro Gly 35 40 45Ile Gln Met Gly Ser Gly Glu Glu His Arg Leu Asp Ala His Lys Lys 50 55 60Leu Leu Ile Gly Leu Ile Ile Ser Ser Ser Ser Leu Gly Ile Val Ile65 70 75 80Leu Ile Cys Phe Gly Phe Trp Met Tyr Cys Arg Lys Lys Ala Pro Lys 85 90 95Pro Ile Lys Ile Pro Asp Ala Glu Ser Gly Thr Ser Ser Phe Ser Met 100 105 110Phe Val Arg Arg Leu Ser Ser Ile Lys Thr Gln Arg Thr Ser Ser Asn 115 120 125Gln Gly Tyr Val Gln Arg Phe Asp Ser Lys Thr Leu Glu Lys Ala Thr 130 135 140Gly Gly Phe Lys Asp Ser Asn Val Ile Gly Gln Gly Gly Phe Gly Cys145 150 155 160Val Tyr Lys Ala Ser Leu Asp Ser Asn Thr Lys Ala Ala Val Lys Lys 165 170 175Ile Glu Asn Val Ser Gln Glu Ala Lys Arg Glu Phe Gln Asn Glu Val 180 185 190Glu Leu Leu Ser Lys Ile Gln His Ser Asn Ile Ile Ser Leu Leu Gly 195 200 205Ser Ala Ser Glu Ile Asn Ser Ser Phe Val Val Tyr Glu Leu Met Glu 210 215 220Lys Gly Ser Leu Asp Asp Gln Leu His Gly Pro Ser Cys Gly Ser Ala225 230 235 240Leu Thr Trp His Met Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly 245 250 255Leu Glu Tyr Leu His Glu His Cys Arg Pro Pro Val Ile His Arg Asp 260 265 270Leu Lys Ser Ser Asn Ile Leu Leu Asp Ser Ser Phe Asn Ala Lys Ile 275 280 285Ser Asp Phe Gly Leu Ala Val Ser Val Gly Val His Gly Ser Asn Asn 290 295 300Ile Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu305 310 315 320Asp Gly Lys Leu Thr Asp Lys Ser Asp Val Tyr Ala Phe Gly Val Val 325 330 335Leu Leu Glu Leu Leu Leu Gly Arg Arg Pro Val Glu Lys Leu Ser Pro 340 345 350Ser Gln Cys Gln Ser Leu Val Thr Trp Ala Met Pro Gln Leu Thr Asp 355 360 365Arg Ser Lys Leu Pro Asn Ile Val Asp Pro Val Ile Lys Asp Thr Met 370 375 380Asp Leu Lys His Leu Tyr Gln Val Ala Ala Met Ala Val Leu Cys Val385 390 395 400Gln Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser 405 410 415Leu Val Pro Leu Val Pro Val Glu Leu Gly Gly Thr Leu Arg Leu Thr 420 425 430Arg19531DNAARTIFICIAL SEQUENCESynthetic Primer 195aatccagctc attctggaat tccttctcgc a 3119632DNAARTIFICIAL SEQUENCESynthetic Primer 196tgaacttgct caggattggc accagtgtga tc 32197461PRTBRACHYPODIUM DISTACHYON 197Met Glu Ile Pro Ala Ala Pro Pro Pro Pro Leu Pro Val Leu Cys Ser1 5 10 15Tyr Val Val Phe Leu Leu Leu Leu Ser Ser Cys Ser Leu Ala Arg Gly 20 25 30Arg Ile Ala Val Ser Ser Pro Gly Pro Ser Pro Val Ala Ala Ala Val 35 40 45Thr Ala Asn Glu Thr Ala Ser Ser Ser Ser Ser Pro Val Phe Pro Ala 50 55 60Ala Pro Pro Val Val Ile Thr Val Val Arg His His His Tyr His Arg65 70 75 80Glu Leu Val Ile Ser Ala Val Leu Ala Cys Val Ala Thr Ala Met Ile 85 90 95Leu Leu Ser Thr Leu Tyr Ala Trp Thr Met Trp Arg Arg Ser Arg Arg 100 105 110Thr Pro His Gly Gly Lys Gly Arg Gly Arg Arg Ser Gly Ile Thr Leu 115 120 125Val Pro Ile Leu Ser Lys Phe Asn Ser Val Lys Met Ser Arg Lys Gly 130 135 140Gly Leu Val Thr Met Ile Glu Tyr Pro Ser Leu Glu Ala Ala Thr Gly145 150 155 160Lys Phe Gly Glu Ser Asn Val Leu Gly Val Gly Gly Phe Gly Cys Val 165 170 175Tyr Lys Ala Ala Phe Asp Gly Gly Ala Thr Ala Ala Val Lys Arg Leu 180 185 190Glu Gly Gly Gly Pro Asp Cys Glu Lys Glu Phe Glu Asn Glu Leu Asp 195 200 205Leu Leu Gly Arg Ile Arg His Pro Asn Ile Val Ser Leu Leu Gly Phe 210 215 220Cys Val His Gly Gly Asn His Tyr Ile Val Tyr Glu Leu Met Glu Lys225 230 235 240Gly Ser Leu Glu Thr Gln Leu His Gly Ser Ser His Gly Ser Ala Leu 245 250 255Ser Trp His Val Arg Met Lys Ile Ala Leu Asp Thr Ala Arg Gly Leu 260 265 270Glu Tyr Leu His Glu His Cys Asn Pro Pro Val Ile His Arg Asp Leu 275 280 285Lys Pro Ser Asn Ile Leu Leu Asp Ser Asp Phe Asn Ala Lys Ile Ala 290 295 300Asp Phe Gly Leu Ala Val Thr Gly Gly Asn Leu Asn Lys Gly Asn Leu305 310 315 320Lys Leu Ser Gly Thr Leu Gly Tyr Val Ala Pro Glu Tyr Leu Leu Asp 325 330 335Gly Lys Leu Thr Glu Lys Ser Asp Val Tyr Ala Phe Gly Val Val Leu 340 345 350Leu Glu Leu Leu Met Gly Arg Lys Pro Val Glu Lys Met Ser Pro Ser 355 360 365Gln Cys Gln Ser Ile Val Ser Trp Ala Met Pro Gln Leu Thr Asp Arg 370 375 380Ser Lys Leu Pro Asn Ile Ile Asp Leu Val Ile Lys Asp Thr Met Asp385 390 395 400Pro Lys His Leu Tyr Gln Val Ala Ala Val Ala Val Leu Cys Val Gln 405 410 415Pro Glu Pro Ser Tyr Arg Pro Leu Ile Thr Asp Val Leu His Ser Leu 420 425 430Val Pro Leu Val Pro Ala Glu Leu Gly Gly Thr Leu Arg Val Ala Glu 435 440 445Pro Pro Ser Pro Ser Pro Asp Gln Arg His Tyr Pro Cys 450 455 46019853DNAARTIFICIAL SEQUENCESynthetic Primer 198tataccggta aaatgagaga gcttcttctt cttcttcttc ttcattttca gtc 5319939DNAARTIFICIAL SEQUENCESynthetic Primer 199atataccggt cttgttaacc ggagagtccc tcctagctc 3920018DNAARTIFICIAL SEQUENCESynthetic Primer 200cgctcctccc gtcgtgat 18

Claims

1. A method of increasing water use efficiency in a plant, comprising: a) introducing a nucleic acid construct to a plant, a plant tissue culture or a plant cell to obtain a modified plant, a modified plant tissue culture or a modified plant cell, wherein the nucleic acid construct inhibits the expression or activity of a PK220 gene; b) growing the modified plant or regenerating a plant from the modified plant tissue culture or the modified plant cell; and c) selecting a plant having increased water use efficiency relative to a wild type plant.

2. The method of claim 1, wherein the nucleic acid construct comprises an antisense sequence, siRNA, hairpin, miRNA, artificial miRNA, each of which is complementary to a nucleic acid sequence encoding a PK220 polypeptide.

3. The method of claim 1, wherein the nucleic acid construct comprises a sequence of at least 19 nucleotides.

4. A plant produced by the method of claim 1, wherein the plant has increased water use efficiency relative to a wild type plant.

5. A seed produced by the plant of claim 4, wherein the seed produces a plant having increased water use efficiency relative to a wild type plant.

6. A method of producing a drought tolerant plant, comprising: a) introducing a nucleic acid construct to a plant, a plant tissue culture or a plant cell to obtain a modified plant, a modified plant tissue culture or a modified plant cell, wherein the nucleic acid construct inhibits the expression or activity of a PK220 gene; b) growing the modified plant or regenerating a plant from the modified plant tissue culture or the modified plant cell; and c) selecting a plant having increased drought tolerance relative to a wild type plant.

7. The method of claim 6, wherein the nucleic acid construct comprises an antisense sequence, siRNA, hairpin, miRNA, artificial miRNA, each of which is complementary to a nucleic acid sequence encoding a PK220 polypeptide.

8. The method of claim 6, wherein the nucleic acid construct comprises a sequence of at least 19 nucleotides.

9. A plant produced by the method of claim 6, wherein the plant has increased drought tolerance relative to a wild type plant.

10. A seed produced by the plant of claim 9, wherein the seed produces a plant having increased drought tolerance relative to a wild type plant.

11. A method of producing a cold stress tolerant plant, comprising: a) introducing a nucleic acid construct to a plant, a plant tissue culture or a plant cell to obtain a modified plant, a modified plant tissue culture or a modified plant cell, wherein the nucleic acid construct inhibits the expression or activity of a PK220 gene; b) growing the modified plant or regenerating a plant from the modified plant tissue culture or the modified plant cell; and c) selecting a plant having increased cold stress tolerance relative to a wild type plant.

12. The method of claim 11, wherein the nucleic acid construct comprises an antisense sequence, siRNA, hairpin, miRNA, artificial miRNA, each of which is complementary to a nucleic acid sequence encoding a PK220 polypeptide.

13. The method of claim 11, wherein the nucleic acid construct comprises a sequence of at least 19 nucleotides.

14. A plant produced by the method of claim 11, wherein the plant has increased cold stress tolerance relative to a wild type plant.

15. A seed produced by the plant of claim 14, wherein the seed produces a plant having increased cold stress tolerance relative to a wild type plant.

16. A method of producing a plant tolerant to low nitrogen conditions, comprising: a) introducing a nucleic acid construct to a plant, a plant tissue culture or a plant cell to obtain a modified plant, a modified plant tissue culture or a modified plant cell, wherein the nucleic acid construct inhibits the expression or activity of a PK220 gene; b) growing the modified plant or regenerating a plant from the modified plant tissue culture or the modified plant cell; and c) selecting a plant having increased tolerance to low nitrogen conditions relative to a wild type plant.

17. The method of claim 16, wherein the nucleic acid construct comprises an antisense sequence, siRNA, hairpin, miRNA, artificial miRNA, each of which is complementary to a nucleic acid sequence encoding a PK220 polypeptide.

18. The method of claim 16, wherein the nucleic acid construct comprises a sequence of at least 19 nucleotides.

19. A plant produced by the method of claim 16, wherein the plant has increased tolerance to low nitrogen conditions relative to a wild type plant.

20. A seed produced by the plant of claim 19, wherein the seed produces a plant having increased tolerance to low nitrogen conditions relative to a wild type plant.

21. The method of claim 1, wherein the nucleic acid construct introduces a mutation into the PK220 gene, thereby inhibiting the expression or activity of the PK220 gene.

22. The method of claim 6, wherein the nucleic acid construct introduces a mutation into the PK220 gene, thereby inhibiting the expression or activity of the PK220 gene.

23. The method of claim 11, wherein the nucleic acid construct introduces a mutation into the PK220 gene, thereby inhibiting the expression or activity of the PK220 gene.

24. The method of claim 16, wherein the nucleic acid construct introduces a mutation into the PK220 gene, thereby inhibiting the expression or activity of the PK220 gene.

25. A method of modifying a plant genome, comprising introducing a nucleic acid construct to a plant, a plant tissue culture or a plant cell having a PK220 gene to obtain a modified plant, a modified plant tissue culture or a modified plant cell, wherein the nucleic acid construct introduces a mutation into the PK220 gene.