TRANSFORMED PLANT AND METHOD FOR PRODUCING EXUDATE CONTAINING SUGAR USING TRANSFORMED PLANT

Abstract

The production of exudate containing sugar from a plant at a high concentration is provided. A nucleic acid encoding a transporter involved in sugar transportation having a certain consensus sequence derived from the amino acid sequences of the SWEET proteins classified in the clade III is introduced and/or expression of the protein is enhanced.

Description

CROSS REFERENCE TO RELATED APPLICATIONS

[0001] This application is a Divisional of U.S. application Ser. No. 15/107,998 filed Jun. 24, 2016, which is a National Stage of International Application No. PCT/JP2014/084316 filed Dec. 25, 2014, claiming priority based on Japanese Patent Application No. 2013-273128 filed Dec. 27, 2013, the contents of all of which are incorporated herein by reference in their entirety.

TECHNICAL FIELD

[0002] The present invention relates to a transformed plant that has gained an excellent trait by introduction of a given gene and a method for producing an exudate containing sugar using the transformed plant.

BACKGROUND ART

[0003] For stable production of biofuel or bioplastics, low cost and stable supply of their raw material sugar is desired. The representative example of the raw material sugar is sugar accumulated in sugarcane. Extraction of sugar from sugarcane generally requires processes such as cutting down of sugarcane at a predetermined harvest time, crushing, pressing, concentration, and purification. Moreover, after harvest, the farmland requires management work such as maintenance of farm for new cultivation, planting, and spraying herbicides and insecticides. The production of the raw material sugar with plants such as sugarcane has been conventionally a process requiring a great deal of cost such as that for the production process and the cultivation, as described above.

[0004] Patent Literature 1 discloses a method for recovering a heterologous protein encoded by a heterologous gene from a plant transformed to express the heterologous gene. The method disclosed in Patent Literature 1 comprises collecting an exudate from a plant transformed to express a heterologous gene and recovering the heterologous protein from the collected exudate. Examples of the exudate in Patent Literature 1 include exudate from the rhizome and the guttation exuded from a plant as an exudate through the hydathode of the leaf

[0005] Patent Literature 2 and Non Patent Literature 1 disclose transporter proteins involved in sugar transport in plant in Arabidopsis thaliana and rice (Oryza sativa). The transporter proteins disclosed in Patent Literature 2 and Non Patent Literature 1 are known as GLUE proteins or SWEET proteins. Introduction of a nucleic acid encoding a transporter protein disclosed in Patent Literature 2 and Non Patent Literature 1 into a plant may improve the amount of sugar transport to root.

[0006] Non Patent Literature 2 describes the confirmation of function of a cell membrane small molecule transporter by artificially localizing the cell membrane transporter on the endoplasmic reticulum (ER) and measuring the small molecule transporter activity of the ER. In particular, the glucose transporters GLUTs and SGLTs were localized on the ER and their original functions were speculated using FRET (Forster resonance energy transfer or fluorescence resonance energy transfer).

CITATION LIST

Patent Literature

Patent Literature 1

[0007] JP Patent Publication (Kohyou) No. 2002-501755 A

Patent Literature 2

[0008] JP Patent Publication (Kohyou) No. 2012-525845 A

Non Patent Literature

Non Patent Literature 1

[0009] Nature (2010) 468, 527-534

Non Patent Literature 2

[0010] FASEB J. (2010) 24, 2849-2858

SUMMARY OF INVENTION

Technical Problem

[0011] As described in the foregoing, large cost of producing sugar using plants has been a big problem. The aforementioned problem may be however solved by including sugar at a high concentration in the exudate derived from a plant and collecting the exudate. Patent Literature 1 discloses the collection of a heterologous protein from exudate, but no technique to collect sugar from the exudate. Patent Literature 2 and Non Patent Literature 1 disclose the transporter proteins, designated as SWEETs, involved in sugar transportation and nucleic acids encoding them, but no relation between these transporter proteins or nucleic acids encoding them and the sugar content in the exudate.

[0012] Accordingly, in view of the circumstances described above, an object of the present invention is to provide a transformed plant that produces an exudate containing sugar at a high concentration and a method for producing sugar using the transformed plant.

Solution to Problem

[0013] As a result of diligent studies to achieve the purpose described above, the present inventors have found that high sugar contents in exudate are achieved in the transformed plant in which a nucleic acid encoding a SWEET protein in a certain group (clade) is introduced and expression of the protein is enhanced, thereby completing the present invention.

[0014] The present invention encompasses the following:

[0015] (1) A transformed plant or a transformed plant cell in which a nucleic acid encoding a transporter protein having a consensus sequence comprising the following amino acid sequence: (L/V/M/M/F)x(G/A)xx(I/L/V/M/F)xxxx(L/V/M/F)(A/S)(P/S) [SEQ ID NO: 1] (1-3aa)(P/S/T/A)T(F/L)xx(I/V)xxxKxxxxxxxxPYxxx(L/I)xxxx(L/I)x(I/L/M/V/F)x- Y(A/S/G) [SEQ ID NO: 2] (7-13aa)(I/L/V/M)(1-2aa)(I/V)Nxxxxxx(E/Q)xxYxxx(Y/F)xx(Y/F)(A/G/S) [SEQ ID NO: 3] (35-36aa)(R/Q/H)xxxxGx(V/I/L)xxxxx(V/M/L/I/F)xxxx(A/S/T)P(L/M)x- (I/V)(I/MN/L) [SEQ ID NO: 4] (2-7aa)(V/I)(V/I/M)x(T/S)x(S/N)xx(F/Y)(M/L)(P/S)(F/I/V/L)xLSxx(L/I)(T/V)x- x(A/G)xxW(F/L) xYGxxxxDxx(V/I)xxPNxxGxx(F/L)(G/S)xxQ(M/I)x(L/M/I/V/F)(Y/H/F) [SEQ ID NO: 5] and being involved in sugar transportation is introduced and/or expression of the protein is enhanced.

[0016] (2) The transformed plant or transformed plant cell according to (1), wherein the transporter protein is a protein in the clade III among the clades I to V of taxonomic groups based on the amino acid sequences of the SWEET proteins.

[0017] (3) The transformed plant or transformed plant cell according to (1), wherein the transporter protein is a protein of any of the following (a) and (b):

[0018] (a) a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 137;

[0019] (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any of SEQ ID NOs: 15 to 137 and having transporter activity involved in sugar transportation.

[0020] (4) The transformed plant or transformed plant cell according to (1), wherein the consensus sequence comprises the following amino acid sequence:

TABLE-US-00001 [SEQ ID NO: 6] G(L/I/V/F/M)xGx(I/V/L)(I/V/L)(S/T)xxxxL(A/S)P(L/ V/I/M)(P/S/T/A)TFxx(I/V)x(K/R)xK(S/T)xxx(F/Y)x (S/A)xPYxx(A/S/T)LxSxxLx(L/I/M/V)(Y/F)Y(A/G) [SEQ ID NO: 7] (7-9 aa)(L/I)(I/V/L)(T/S)INxx(G/A)xx(I/V/M)(E/Q) xxYxxx(F/Y)(L/I/V/F)x(Y/F)Ax(K/R/N)xxxxx(T/A) (7-8 aa) [SEQ ID NO: 8] (V/F/L/I/M)(18-19 aa)(R/Q/H)xxxxGx(I/V)xxxxx(V/I/ L/M)x(V/M)F(A/V)(A/S/T)PLx(I/V)(I/M/V/L)xxV(I/V) (K/R/Q)(T/S)(K/R)S (V/A)x(F/Y)MP(F/I/L)xLS(L/F/V) xL(T/V)(L/I)xAxxW(F/L)xYG(L/F)xxxDxx(V/I)xxPNxxGxx (L/F)(G/S)xxQMx(L/V/I)(Y/F)xx(Y/F).

[0021] (5) The transformed plant or transformed plant cell according to (4), wherein the transporter protein is a protein of any of the following (a) and (b):

[0022] (a) a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 35;

[0023] (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any of SEQ ID NOs: 15 to 35 and having transporter activity involved in sugar transportation.

[0024] (6) The transformed plant or transformed plant cell according to (1), wherein the consensus sequence comprises the following amino acid sequence:

TABLE-US-00002 [SEQ ID NO: 9] (A/V)xxxG(I/L/V)xGN(I/L/V)(I/L/V)S(F/L)x(V/T)xL(A/ S)P(V/L/I)(P/A)TFxx(I/V)x(K/R)xK(S/T)xx(G/S)(F/Y) (Q/S/E)SxPYxx(A/S/T)LxS(A/C/S)xLx(L/I/M)(Y/F)Y (A/G)xx(K/T) [SEQ ID NO: 10] (3-5 aa)(L/M/P)(L/I)(I/L/V)(T/S)INxx(G/A)xx(I/V) (E/Q)xxY(I/L)x(L/M/V/I)(F/Y)(L/I/V/F)x(Y/F)Ax(K/R) xxxxx(T/A)xx(L/M/F/V/I)(L/F/V/I)xxx(N/D)(F/V/I/L) xx(F/L)xx(I/L/V)xxxxxx(L/I/V) [SEQ ID NO: 11] (5-6 aa)(R/Q)xxxxGx(I/V)xxxx(S/A)(V/L/M)(C/S/A)VF (A/V)(A/S)PLx(I/V)(I/M/V)xxV(I/V)(K/R/Q)(T/S)(K/R) S(V/A)E(F/Y)MP(F/I)xL S(L/F/V)xL(T/V)(L/I)(S/N)A (V/I)xW(F/L)xYGLxx(K/N)Dxx(V/I)xxPN(V/I)xGxx(F/L) (G/S)xxQMxL(Y/F)xx(Y/F).

[0025] (7) The transformed plant or transformed plant cell according to (6), wherein the transporter protein is a protein of any of the following (a) and (b):

[0026] (a) a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 26;

[0027] (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any of SEQ ID NOs: 15 to 26 and having transporter activity involved in sugar transportation.

[0028] (8) The transformed plant or transformed plant cell according to (1), wherein the consensus sequence comprises the following amino acid sequence:

TABLE-US-00003 [SEQ ID NO: 12] (M/L/V)xx(T/K/N/S)xxxxAxxFG(L/I/V)LGN(I/L/V)(I/V) SFxVxL(S/A)P(V/I)PTFxxIxK(K/R)K(S/T)x(E/K)(G/S)(F/ Y)(Q/E)S(I/L)PYxx(A/S)LxS(A/C)xLx(L/I/M)YY(A/G)xxK [SEQ ID NO: 13] (4-5 aa)(L/M)(L/I)(I/V)(T/S)IN(A/S/T)(F/V)(G/A)x (F/V)(I/V)(E/Q)xxY(I/L)x(L/M/I)(F/Y)(F/V/I/L)x(Y/ F)Ax(K/R)xx(R/K)xx(T/A)(L/V/M)K(V/L/M/F)(L/I/V/F) xxx(N/D)(F/V/I)xx(F/L)xx(I/L)(L/I/V/F)(L/M/V)(L/V) xx(F/L)(L/I/V) [SEQ ID NO: 14] (5-6 aa)(R/Q)x(K/S/Q)x(L/I/V)Gx(I/V)Cxxx(S/A)(V/L) (S/C/A)VF(A/V)(A/S)PLx(I/V)(M/I/V)xxV(I/V)(K/R)T (K/R)S(V/A)E(Y/F)MPFxLS(L/F)xLT(I/L)(S/N)A(V/I)xW (L/F)xYGLx(L/I)(K/N)Dxx(V/I)A(L/F/I/M)PN(V/I)(L/ I/V)Gxx(L/F)GxxQM(I/V)L(Y/F)(V/L/I/M)(V/L/I/M)(Y/ F)(K/R/Q).

[0029] (9) The transformed plant or transformed plant cell according to (8), wherein the transporter protein is a protein of any of the following (a) and (b):

[0030] (a) a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 21;

[0031] (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any of SEQ ID NOs: 15 to 21 and having transporter activity involved in sugar transportation.

[0032] (10) The transformed plant or transformed plant cell according to (1), wherein the transformed plant is a phanerogam.

[0033] (11) The transformed plant or transformed plant cell according to (10), wherein the phanerogam is an angiosperm.

[0034] (12) The transformed plant or transformed plant cell according to (11), wherein the angiosperm is a monocot.

[0035] (13) The transformed plant or transformed plant cell according to (12), wherein the monocot is a plant of the family Poaceae.

[0036] (14) The transformed plant or transformed plant cell according to (13), wherein the plant of the family Poaceae is a plant of the genus Oryza.

[0037] (15) The transformed plant or transformed plant cell according to (11), wherein the angiosperm is a dicot.

[0038] (16) The transformed plant or transformed plant cell according to (15), wherein the dicot is a plant of the family Brassicaceae.

[0039] (17) The transformed plant or transformed plant cell according to (16), wherein the plant of the family Brassicaceae is a plant of the genus Arabidopsis.

[0040] (18) A method for producing an exudate, comprising the steps of cultivating a transformed plant according to any of the above (1) to (17); and collecting an exudate from the transformed plant.

[0041] (19) A method for producing an exudate according to (18), wherein the transformed plant is cultivated under conditions at a relative humidity of 80% RH or more.

[0042] (20) The method for producing an exudate according to (18), wherein the exudate is guttation.

[0043] The description of the present application encompasses the contents described in the description and/or the drawings of JP patent application No. 2013-273128, which is the basics of the priority of the present application.

Advantageous Effects of Invention

[0044] According to the present invention, the sugar content in the exudate derived from plants can be greatly increased. Accordingly, transformed plants according to the present invention can produce exudate having a property such as high sugar content by introducing a nucleic acid encoding a particular transporter protein involved in sugar transportation and/or enhancing expression of the protein. Also, the method for producing an exudate according to the present invention can produce an exudate with a high sugar content by using a transformed plant in which a nucleic acid encoding a particular transporter protein involved in sugar transportation is introduced and/or expression of the protein is enhanced. Furthermore, the exudate collected from the transformed plant can be used as a raw material for producing alcohol, organic acid, alkane, and terpenoids because of its high sugar content.

BRIEF DESCRIPTION OF DRAWINGS

[0045] FIG. 1-1 is a schematic view of a phylogenetic tree made based on the information of amino acid sequences of SWEET proteins in the clade III defined in Non-Patent Literature 1 (Nature (2010) 468, 527-532) collected from the GenBank database provided at National Center for Biotechnology Information (NCBI).

[0046] FIG. 1-2 is an extended view of a part of the phylogenetic tree shown in FIG. 1-1.

[0047] FIG. 1-3 is an extended view of a part of the phylogenetic tree shown in FIG. 1-1.

[0048] FIG. 2-1 illustrates a result of multiple alignment analysis of the proteins contained in the phylogenetic tree illustrated in FIG. 1-1[XP 004235326 Solanum (SEQ ID NO: 117), XP 004235334 Solanum (SEQ ID NO: 119), ACV71016 Capsicum (SEQ ID NO: 36), XP 004235333 Solanum (SEQ ID NO: 118), XP 004235342 Solanum (SEQ ID NO: 122), XP 004235339 Solanum (SEQ ID NO: 120), XP 004241452 Solanum (SEQ ID NO: 124), XP 004235340 Solanum (SEQ ID NO: 121), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 004503778 Cicer (SEQ ID NO: 131), AFK48645 Lotus (SEQ ID NO: 38), NP 001241307 Glycine (SEQ ID NO: 75), NP 001242732 Glycine (SEQ ID NO: 76), XP 003523161 Glycine (SEQ ID NO: 99), NP 001237418 Glycine (SEQ ID NO: 73), XP 003602780 Medicago (SEQ ID NO: 28), XP 004138032 Cucumis (SEQ ID NO: 109), EMJ10621 Prunus (SEQ ID NO: 49), XP 004297512 Fragaria (SEQ ID NO: 127), XP 002284244 Vitis (SEQ ID NO: 79), EOA14646 Capsella (SEQ ID NO: 61), NP 199892 AtSW10 (SEQ ID NO: 16), XP 002321731 Populus (SEQ ID NO: 82), XP 002322281 Populus (SEQ ID NO: 83), XP 002321730 Populus (SEQ ID NO: 81), XP 002511127 Ricinus (SEQ ID NO: 93), XP 002511128 Ricinus (SEQ ID NO: 94), CBI32263 Vitis (SEQ ID NO: 46), EMJ01437 Prunus (SEQ ID NO: 48), XP 002520679 Ricinus (SEQ ID NO: 96), XP 004247459 Solanum (SEQ ID NO: 125), EOA28959 Capsella (SEQ ID NO: 67), NP 181439 AtSW09 (SEQ ID NO: 15), XP 002333315 Populus (SEQ ID NO: 84), NEC1 PETHY Petunia (SEQ ID NO: 35), XP 002267792 Vitis (SEQ ID NO: 78), XP 004138978 Cucumis (SEQ ID NO: 111), XP 004138979 Cucumis (SEQ ID NO: 112), XP 003518628 Glycine (SEQ ID NO: 98), XP 004489106 Cicer (SEQ ID NO: 130), XP 003617528 Medicago (SEQ ID NO: 27), XP 004302124 Fragaria (SEQ ID NO: 129), NOD3 MEDTR Medicago (SEQ ID NO: 31), NP 001239695 Glycine (SEQ ID NO: 74), AFK39311 Lotus (SEQ ID NO: 37), XP 003620983 Medicago (SEQ ID NO: 32), XP 003530901 Glycine (SEQ ID NO: 101), XP 003524088 Glycine (SEQ ID NO: 100), XP 003615405 Medicago (SEQ ID NO: 33), XP 003547573 Glycine (SEQ ID NO: 102)].

[0049] FIG. 2-2 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following below FIG. 2-1 [XP 003593107 Medicago (SEQ ID NO: 34), EOA22072 Capsella (SEQ ID NO: 65), NP 196821 AtSW15 (SEQ ID NO: 21), EMJ23678 Prunus (SEQ ID NO: 50), XP 004301046 Fragaria (SEQ ID NO: 128), XP 002299333 Populus (SEQ ID NO: 80), XP 002514863 Ricinus (SEQ ID NO: 95), XP 004140547 Cucumis (SEQ ID NO: 113), XP 002264875 Vitis (SEQ ID NO: 77), NP 199893 AtSW13 (SEQ ID NO: 19), XP 002862913 Arabiopsis (SEQ ID NO: 97), EOA14916 Capsella (SEQ ID NO: 62), EOA17919 Capsella (SEQ ID NO: 63), NP 194231 AtSW14 (SEQ ID NO: 20), EOA21276 Capsella (SEQ ID NO: 64), NP 197755 AtSW12 (SEQ ID NO: 18), EOA24501 Capsella (SEQ ID NO: 66), NP 190443 AtSW11 (SEQ ID NO: 17), XP 002511126 Ricinus (SEQ ID NO: 92), XP 004297511 Fragaria (SEQ ID NO: 126), XP 004153501 Cucumis (SEQ ID NO: 115), XP 004161952 Cucumis (SEQ ID NO: 116), XP 004145146 Cucumis (SEQ ID NO: 114), XP 004138250 Cucumis (SEQ ID NO: 110), XP 004235470 Solanum (SEQ ID NO: 123), CBI15715 Vitis (SEQ ID NO: 45), AFW71563 Zea (SEQ ID NO: 39), NP 001149028 Zea (SEQ ID NO: 72), XP 002453892 Sorghum (SEQ ID NO: 89), EMT09236 Aegilops (SEQ ID NO: 54), XP 003575028 Brachypodium (SEQ ID NO: 105), NP 001046944 OsSW15 (SEQ ID NO: 26), EMS46194 Triticum (SEQ ID NO: 52), AFW88409 Zea (SEQ ID NO: 40), XP 002465280 Sorghum (SEQ ID NO: 91), BAJ99068 Hordeum (SEQ ID NO: 43), EMT31030 Aegilops (SEQ ID NO: 59), XP 003561640 Brachypodium (SEQ ID NO: 103), NP 001050099 OsSW12 (SEQ ID NO: 23), BAK07340 Hordeum (SEQ ID NO: 44), EMS45810 Triticum (SEQ ID NO: 51), XP 003578398 Brachypodium (SEQ ID NO: 108), XP 002462642 Sorghum (SEQ ID NO: 90), EAZ09693 Oryza (SEQ ID NO: 47), NP 001148964 Zea (SEQ ID NO: 71), XP 002444688 Sorghum (SEQ ID NO: 87), XP 003572455 Brachypodium (SEQ ID NO: 104), NP 001062354 OsSW11 (SEQ ID NO: 22), EMT31640 Aegilops (SEQ ID NO: 60), EMS51422 Triticum (SEQ ID NO: 53), EMT20808 Aegilops (SEQ ID NO: 58)].

[0050] FIG. 2-3 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following below FIG. 2-2 [BAJ85621 Hordeum (SEQ ID NO: 41), EMT11081 Aegilops (SEQ ID NO: 55), XP 002442119 Sorghum (SEQ ID NO: 85), XP 002443167 Sorghum (SEQ ID NO: 86), NP 001141654 Zea (SEQ ID NO: 70), NP 001141106 Zea (SEQ ID NO: 69), SWT13 ORYSJ OsSW13 Oryza (SEQ ID NO: 24), XP 003576225 Brachypodium (SEQ ID NO: 107), BAJ94651 Hordeum (SEQ ID NO: 42), XP 003576036 Brachypodium (SEQ ID NO: 106), EMT20480 Aegilops (SEQ ID NO: 56), EMT20481 Aegilops (SEQ ID NO: 57), NP 001132836 Zea (SEQ ID NO: 68), XP 002450786 Sorghum (SEQ ID NO: 88), NP 001067955 OsSW14 (SEQ ID NO: 25)].

[0051] FIG. 2-4 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-1 [XP 004235326 Solanum (SEQ ID NO: 117), XP 004235334 Solanum (SEQ ID NO: 119), ACV71016 Capsicum (SEQ ID NO: 36), XP 004235333 Solanum (SEQ ID NO: 118), XP 004235342 Solanum (SEQ ID NO: 122), XP 004235339 Solanum (SEQ ID NO: 120), XP 004241452 Solanum (SEQ ID NO: 124), XP 004235340 Solanum (SEQ ID NO: 121), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 004503778 Cicer (SEQ ID NO: 131), AFK48645 Lotus (SEQ ID NO: 38), NP 001241307 Glycine (SEQ ID NO: 75), NP 001242732 Glycine (SEQ ID NO: 76), XP 003523161 Glycine (SEQ ID NO: 99), NP 001237418 Glycine (SEQ ID NO: 73), XP 003602780 Medicago (SEQ ID NO: 28), XP 004138032 Cucumis (SEQ ID NO: 109), EMJ10621 Prunus (SEQ ID NO: 49), XP 004297512 Fragaria (SEQ ID NO: 127), XP 002284244 Vitis (SEQ ID NO: 79), EOA14646 Capsella (SEQ ID NO: 61), NP 199892 AtSW10 (SEQ ID NO: 16), XP 002321731 Populus (SEQ ID NO: 82), XP 002322281 Populus (SEQ ID NO: 83), XP 002321730 Populus (SEQ ID NO: 81), XP 002511127 Ricinus (SEQ ID NO: 93), XP 002511128 Ricinus (SEQ ID NO: 94), CBI32263 Vitis (SEQ ID NO: 46), EMJ01437 Prunus (SEQ ID NO: 48), XP 002520679 Ricinus (SEQ ID NO: 96), XP 004247459 Solanum (SEQ ID NO: 125), EOA28959 Capsella (SEQ ID NO: 67), NP 181439 AtSW09 (SEQ ID NO: 15), XP 002333315 Populus (SEQ ID NO: 84), NEC1 PETHY Petunia (SEQ ID NO: 35), XP 002267792 Vitis (SEQ ID NO: 78), XP 004138978 Cucumis (SEQ ID NO: 111), XP 004138979 Cucumis (SEQ ID NO: 112), XP 003518628 Glycine (SEQ ID NO: 98), XP 004489106 Cicer (SEQ ID NO: 130), XP 003617528 Medicago (SEQ ID NO: 27), XP 004302124 Fragaria (SEQ ID NO: 129), NOD3 MEDTR Medicago (SEQ ID NO: 31), NP 001239695 Glycine (SEQ ID NO: 74), AFK39311 Lotus (SEQ ID NO: 37), XP 003620983 Medicago (SEQ ID NO: 32), XP 003530901 Glycine (SEQ ID NO: 101), XP 003524088 Glycine (SEQ ID NO: 100), XP 003615405 Medicago (SEQ ID NO: 33), XP 003547573 Glycine (SEQ ID NO: 102)].

[0052] FIG. 2-5 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-2 [XP 003593107 Medicago (SEQ ID NO: 34), EOA22072 Capsella (SEQ ID NO: 65), NP 196821 AtSW15 (SEQ ID NO: 21), EMJ23678 Prunus (SEQ ID NO: 50), XP 004301046 Fragaria (SEQ ID NO: 128), XP 002299333 Populus (SEQ ID NO: 80), XP 002514863 Ricinus (SEQ ID NO: 95), XP 004140547 Cucumis (SEQ ID NO: 113), XP 002264875 Vitis (SEQ ID NO: 77), NP 199893 AtSW13 (SEQ ID NO: 19), XP 002862913 Arabiopsis (SEQ ID NO: 97), EOA14916 Capsella (SEQ ID NO: 62), EOA17919 Capsella (SEQ ID NO: 63), NP 194231 AtSW14 (SEQ ID NO: 20), EOA21276 Capsella (SEQ ID NO: 64), NP 197755 AtSW12 (SEQ ID NO: 18), EOA24501 Capsella (SEQ ID NO: 66), NP 190443 AtSW11 (SEQ ID NO: 17), XP 002511126 Ricinus (SEQ ID NO: 92), XP 004297511 Fragaria (SEQ ID NO: 126), XP 004153501 Cucumis (SEQ ID NO: 115), XP 004161952 Cucumis (SEQ ID NO: 116), XP 004145146 Cucumis (SEQ ID NO: 114), XP 004138250 Cucumis (SEQ ID NO: 110), XP 004235470 Solanum (SEQ ID NO: 123), CBI15715 Vitis (SEQ ID NO: 45), AFW71563 Zea (SEQ ID NO: 39), NP 001149028 Zea (SEQ ID NO: 72), XP 002453892 Sorghum (SEQ ID NO: 89), EMT09236 Aegilops (SEQ ID NO: 54), XP 003575028 Brachypodium (SEQ ID NO: 105), NP 001046944 OsSW15 (SEQ ID NO: 26), EMS46194 Triticum (SEQ ID NO: 52), AFW88409 Zea (SEQ ID NO: 40), XP 002465280 Sorghum (SEQ ID NO: 91), BAJ99068 Hordeum (SEQ ID NO: 43), EMT31030 Aegilops (SEQ ID NO: 59), XP 003561640 Brachypodium (SEQ ID NO: 103), NP 001050099 OsSW12 (SEQ ID NO: 23), BAK07340 Hordeum (SEQ ID NO: 44), EMS45810 Triticum (SEQ ID NO: 51), XP 003578398 Brachypodium (SEQ ID NO: 108), XP 002462642 Sorghum (SEQ ID NO: 90), EAZ09693 Oryza (SEQ ID NO: 47), NP 001148964 Zea (SEQ ID NO: 71), XP 002444688 Sorghum (SEQ ID NO: 87), XP 003572455 Brachypodium (SEQ ID NO: 104), NP 001062354 OsSW11 (SEQ ID NO: 22), EMT31640 Aegilops (SEQ ID NO: 60), EMS51422 Triticum (SEQ ID NO: 53), EMT20808 Aegilops (SEQ ID NO: 58)].

[0053] FIG. 2-6 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-3[BAJ85621 Hordeum (SEQ ID NO: 41), EMT11081 Aegilops (SEQ ID NO: 55), XP 002442119 Sorghum (SEQ ID NO: 85), XP 002443167 Sorghum (SEQ ID NO: 86), NP 001141654 Zea (SEQ ID NO: 70), NP 001141106 Zea (SEQ ID NO: 69), SWT13 ORYSJ OsSW13 Oryza (SEQ ID NO: 24), XP 003576225 Brachypodium (SEQ ID NO: 107), BAJ94651 Hordeum (SEQ ID NO: 42), XP 003576036 Brachypodium (SEQ ID NO: 106), EMT20480 Aegilops (SEQ ID NO: 56), EMT20481 Aegilops (SEQ ID NO: 57), NP 001132836 Zea (SEQ ID NO: 68), XP 002450786 Sorghum (SEQ ID NO: 88), NP 001067955 OsSW14 (SEQ ID NO: 25)].

[0054] FIG. 2-7 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-4 [XP 004235326 Solanum (SEQ ID NO: 117), XP 004235334 Solanum (SEQ ID NO: 119), ACV71016 Capsicum (SEQ ID NO: 36), XP 004235333 Solanum (SEQ ID NO: 118), XP 004235342 Solanum (SEQ ID NO: 122), XP 004235339 Solanum (SEQ ID NO: 120), XP 004241452 Solanum (SEQ ID NO: 124), XP 004235340 Solanum (SEQ ID NO: 121), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 004503778 Cicer (SEQ ID NO: 131), AFK48645 Lotus (SEQ ID NO: 38), NP 001241307 Glycine (SEQ ID NO: 75), NP 001242732 Glycine (SEQ ID NO: 76), XP 003523161 Glycine (SEQ ID NO: 99), NP 001237418 Glycine (SEQ ID NO: 73), XP 003602780 Medicago (SEQ ID NO: 28), XP 004138032 Cucumis (SEQ ID NO: 109), EMJ10621 Prunus (SEQ ID NO: 49), XP 004297512 Fragaria (SEQ ID NO: 127), XP 002284244 Vitis (SEQ ID NO: 79), EOA14646 Capsella (SEQ ID NO: 61), NP 199892 AtSW10 (SEQ ID NO: 16), XP 002321731 Populus (SEQ ID NO: 82), XP 002322281 Populus (SEQ ID NO: 83), XP 002321730 Populus (SEQ ID NO: 81), XP 002511127 Ricinus (SEQ ID NO: 93), XP 002511128 Ricinus (SEQ ID NO: 94), CBI32263 Vitis (SEQ ID NO: 46), EMJ01437 Prunus (SEQ ID NO: 48), XP 002520679 Ricinus (SEQ ID NO: 96), XP 004247459 Solanum (SEQ ID NO: 125), EOA28959 Capsella (SEQ ID NO: 67), NP 181439 AtSW09 (SEQ ID NO: 15), XP 002333315 Populus (SEQ ID NO: 84), NEC1 PETHY Petunia (SEQ ID NO: 35), XP 002267792 Vitis (SEQ ID NO: 78), XP 004138978 Cucumis (SEQ ID NO: 111), XP 004138979 Cucumis (SEQ ID NO: 112), XP 003518628 Glycine (SEQ ID NO: 98), XP 004489106 Cicer (SEQ ID NO: 130), XP 003617528 Medicago (SEQ ID NO: 27), XP 004302124 Fragaria (SEQ ID NO: 129), NOD3 MEDTR Medicago (SEQ ID NO: 31), NP 001239695 Glycine (SEQ ID NO: 74), AFK39311 Lotus (SEQ ID NO: 37), XP 003620983 Medicago (SEQ ID NO: 32), XP 003530901 Glycine (SEQ ID NO: 101), XP 003524088 Glycine (SEQ ID NO: 100), XP 003615405 Medicago (SEQ ID NO: 33), XP 003547573 Glycine (SEQ ID NO: 102)].

[0055] FIG. 2-8 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-5 [XP 003593107 Medicago (SEQ ID NO: 34), EOA22072 Capsella (SEQ ID NO: 65), NP 196821 AtSW15 (SEQ ID NO: 21), EMJ23678 Prunus (SEQ ID NO: 50), XP 004301046 Fragaria (SEQ ID NO: 128), XP 002299333 Populus (SEQ ID NO: 80), XP 002514863 Ricinus (SEQ ID NO: 95), XP 004140547 Cucumis (SEQ ID NO: 113), XP 002264875 Vitis (SEQ ID NO: 77), NP 199893 AtSW13 (SEQ ID NO: 19), XP 002862913 Arabiopsis (SEQ ID NO: 97), EOA14916 Capsella (SEQ ID NO: 62), EOA17919 Capsella (SEQ ID NO: 63), NP 194231 AtSW14 (SEQ ID NO: 20), EOA21276 Capsella (SEQ ID NO: 64), NP 197755 AtSW12 (SEQ ID NO: 18), EOA24501 Capsella (SEQ ID NO: 66), NP 190443 AtSW11 (SEQ ID NO: 17), XP 002511126 Ricinus (SEQ ID NO: 92), XP 004297511 Fragaria (SEQ ID NO: 126), XP 004153501 Cucumis (SEQ ID NO: 115), XP 004161952 Cucumis (SEQ ID NO: 116), XP 004145146 Cucumis (SEQ ID NO: 114), XP 004138250 Cucumis (SEQ ID NO: 110), XP 004235470 Solanum (SEQ ID NO: 123), CBI15715 Vitis (SEQ ID NO: 45), AFW71563 Zea (SEQ ID NO: 39), NP 001149028 Zea (SEQ ID NO: 72), XP 002453892 Sorghum (SEQ ID NO: 89), EMT09236 Aegilops (SEQ ID NO: 54), XP 003575028 Brachypodium (SEQ ID NO: 105), NP 001046944 OsSW15 (SEQ ID NO: 26), EMS46194 Triticum (SEQ ID NO: 52), AFW88409 Zea (SEQ ID NO: 40), XP 002465280 Sorghum (SEQ ID NO: 91), BAJ99068 Hordeum (SEQ ID NO: 43), EMT31030 Aegilops (SEQ ID NO: 59), XP 003561640 Brachypodium (SEQ ID NO: 103), NP 001050099 OsSW12 (SEQ ID NO: 23), BAK07340 Hordeum (SEQ ID NO: 44), EMS45810 Triticum (SEQ ID NO: 51), XP 003578398 Brachypodium (SEQ ID NO: 108), XP 002462642 Sorghum (SEQ ID NO: 90), EAZ09693 Oryza (SEQ ID NO: 47), NP 001148964 Zea (SEQ ID NO: 71), XP 002444688 Sorghum (SEQ ID NO: 87), XP 003572455 Brachypodium (SEQ ID NO: 104), NP 001062354 OsSW11 (SEQ ID NO: 22), EMT31640 Aegilops (SEQ ID NO: 60), EMS51422 Triticum (SEQ ID NO: 53), EMT20808 Aegilops (SEQ ID NO: 58)].

[0056] FIG. 2-9 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-6 [BAJ85621 Hordeum (SEQ ID NO: 41), EMT11081 Aegilops (SEQ ID NO: 55), XP 002442119 Sorghum (SEQ ID NO: 85), XP 002443167 Sorghum (SEQ ID NO: 86), NP 001141654 Zea (SEQ ID NO: 70), NP 001141106 Zea (SEQ ID NO: 69), SWT13 ORYSJ OsSW13 Oryza (SEQ ID NO: 24), XP 003576225 Brachypodium (SEQ ID NO: 107), BAJ94651 Hordeum (SEQ ID NO: 42), XP 003576036 Brachypodium (SEQ ID NO: 106), EMT20480 Aegilops (SEQ ID NO: 56), EMT20481 Aegilops (SEQ ID NO: 57), NP 001132836 Zea (SEQ ID NO: 68), XP 002450786 Sorghum (SEQ ID NO: 88), NP 001067955 OsSW14 (SEQ ID NO: 25)].

[0057] FIG. 2-10 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-7[XP 004235326 Solanum (SEQ ID NO: 117), XP 004235334 Solanum (SEQ ID NO: 119), ACV71016 Capsicum (SEQ ID NO: 36), XP 004235333 Solanum (SEQ ID NO: 118), XP 004235342 Solanum (SEQ ID NO: 122), XP 004235339 Solanum (SEQ ID NO: 120), XP 004241452 Solanum (SEQ ID NO: 124), XP 004235340 Solanum (SEQ ID NO: 121), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 004503778 Cicer (SEQ ID NO: 131), AFK48645 Lotus (SEQ ID NO: 38), NP 001241307 Glycine (SEQ ID NO: 75), NP 001242732 Glycine (SEQ ID NO: 76), XP 003523161 Glycine (SEQ ID NO: 99), NP 001237418 Glycine (SEQ ID NO: 73), XP 003602780 Medicago (SEQ ID NO: 28), XP 004138032 Cucumis (SEQ ID NO: 109), EMJ10621 Prunus (SEQ ID NO: 49), XP 004297512 Fragaria (SEQ ID NO: 127), XP 002284244 Vitis (SEQ ID NO: 79), EOA14646 Capsella (SEQ ID NO: 61), NP 199892 AtSW10 (SEQ ID NO: 16), XP 002321731 Populus (SEQ ID NO: 82), XP 002322281 Populus (SEQ ID NO: 83), XP 002321730 Populus (SEQ ID NO: 81), XP 002511127 Ricinus (SEQ ID NO: 93), XP 002511128 Ricinus (SEQ ID NO: 94), CBI32263 Vitis (SEQ ID NO: 46), EMJ01437 Prunus (SEQ ID NO: 48), XP 002520679 Ricinus (SEQ ID NO: 96), XP 004247459 Solanum (SEQ ID NO: 125), EOA28959 Capsella (SEQ ID NO: 67), NP 181439 AtSW09 (SEQ ID NO: 15), XP 002333315 Populus (SEQ ID NO: 84), NEC1 PETHY Petunia (SEQ ID NO: 35), XP 002267792 Vitis (SEQ ID NO: 78), XP 004138978 Cucumis (SEQ ID NO: 111), XP 004138979 Cucumis (SEQ ID NO: 112), XP 003518628 Glycine (SEQ ID NO: 98), XP 004489106 Cicer (SEQ ID NO: 130), XP 003617528 Medicago (SEQ ID NO: 27), XP 004302124 Fragaria (SEQ ID NO: 129), NOD3 MEDTR Medicago (SEQ ID NO: 31), NP 001239695 Glycine (SEQ ID NO: 74), AFK39311 Lotus (SEQ ID NO: 37), XP 003620983 Medicago (SEQ ID NO: 32), XP 003530901 Glycine (SEQ ID NO: 101), XP 003524088 Glycine (SEQ ID NO: 100), XP 003615405 Medicago (SEQ ID NO: 33), XP 003547573 Glycine (SEQ ID NO: 102)].

[0058] FIG. 2-11 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-8 [XP 003593107 Medicago (SEQ ID NO: 34), EOA22072 Capsella (SEQ ID NO: 65), NP 196821 AtSW15 (SEQ ID NO: 21), EMJ23678 Prunus (SEQ ID NO: 50), XP 004301046 Fragaria (SEQ ID NO: 128), XP 002299333 Populus (SEQ ID NO: 80), XP 002514863 Ricinus (SEQ ID NO: 95), XP 004140547 Cucumis (SEQ ID NO: 113), XP 002264875 Vitis (SEQ ID NO: 77), NP 199893 AtSW13 (SEQ ID NO: 19), XP 002862913 Arabiopsis (SEQ ID NO: 97), EOA14916 Capsella (SEQ ID NO: 62), EOA17919 Capsella (SEQ ID NO: 63), NP 194231 AtSW14 (SEQ ID NO: 20), EOA21276 Capsella (SEQ ID NO: 64), NP 197755 AtSW12 (SEQ ID NO: 18), EOA24501 Capsella (SEQ ID NO: 66), NP 190443 AtSW11 (SEQ ID NO: 17), XP 002511126 Ricinus (SEQ ID NO: 92), XP 004297511 Fragaria (SEQ ID NO: 126), XP 004153501 Cucumis (SEQ ID NO: 115), XP 004161952 Cucumis (SEQ ID NO: 116), XP 004145146 Cucumis (SEQ ID NO: 114), XP 004138250 Cucumis (SEQ ID NO: 110), XP 004235470 Solanum (SEQ ID NO: 123), CBI15715 Vitis (SEQ ID NO: 45), AFW71563 Zea (SEQ ID NO: 39), NP 001149028 Zea (SEQ ID NO: 72), XP 002453892 Sorghum (SEQ ID NO: 89), EMT09236 Aegilops (SEQ ID NO: 54), XP 003575028 Brachypodium (SEQ ID NO: 105), NP 001046944 OsSW15 (SEQ ID NO: 26), EMS46194 Triticum (SEQ ID NO: 52), AFW88409 Zea (SEQ ID NO: 40), XP 002465280 Sorghum (SEQ ID NO: 91), BAJ99068 Hordeum (SEQ ID NO: 43), EMT31030 Aegilops (SEQ ID NO: 59), XP 003561640 Brachypodium (SEQ ID NO: 103), NP 001050099 OsSW12 (SEQ ID NO: 23), BAK07340 Hordeum (SEQ ID NO: 44), EMS45810 Triticum (SEQ ID NO: 51), XP 003578398 Brachypodium (SEQ ID NO: 108), XP 002462642 Sorghum (SEQ ID NO: 90), EAZ09693 Oryza (SEQ ID NO: 47), NP 001148964 Zea (SEQ ID NO: 71), XP 002444688 Sorghum (SEQ ID NO: 87), XP 003572455 Brachypodium (SEQ ID NO: 104), NP 001062354 OsSW11 (SEQ ID NO: 22), EMT31640 Aegilops (SEQ ID NO: 60), EMS51422 Triticum (SEQ ID NO: 53), EMT20808 Aegilops (SEQ ID NO: 58)].

[0059] FIG. 2-12 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-9 [BAJ85621 Hordeum (SEQ ID NO: 41), EMT11081 Aegilops (SEQ ID NO: 55), XP 002442119 Sorghum (SEQ ID NO: 85), XP 002443167 Sorghum (SEQ ID NO: 86), NP 001141654 Zea (SEQ ID NO: 70), NP 001141106 Zea (SEQ ID NO: 69), SWT13 ORYSJ OsSW13 Oryza (SEQ ID NO: 24), XP 003576225 Brachypodium (SEQ ID NO: 107), BAJ94651 Hordeum (SEQ ID NO: 42), XP 003576036 Brachypodium (SEQ ID NO: 106), EMT20480 Aegilops (SEQ ID NO: 56), EMT20481 Aegilops (SEQ ID NO: 57), NP 001132836 Zea (SEQ ID NO: 68), XP 002450786 Sorghum (SEQ ID NO: 88), NP 001067955 OsSW14 (SEQ ID NO: 25)].

[0060] FIG. 2-13 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-10 [XP 004235326 Solanum (SEQ ID NO: 117), XP 004235334 Solanum (SEQ ID NO: 119), ACV71016 Capsicum (SEQ ID NO: 36), XP 004235333 Solanum (SEQ ID NO: 118), XP 004235342 Solanum (SEQ ID NO: 122), XP 004235339 Solanum (SEQ ID NO: 120), XP 004241452 Solanum (SEQ ID NO: 124), XP 004235340 Solanum (SEQ ID NO: 121), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 004503778 Cicer (SEQ ID NO: 131), AFK48645 Lotus (SEQ ID NO: 38), NP 001241307 Glycine (SEQ ID NO: 75), NP 001242732 Glycine (SEQ ID NO: 76), XP 003523161 Glycine (SEQ ID NO: 99), NP 001237418 Glycine (SEQ ID NO: 73), XP 003602780 Medicago (SEQ ID NO: 28), XP 004138032 Cucumis (SEQ ID NO: 109), EMJ10621 Prunus (SEQ ID NO: 49), XP 004297512 Fragaria (SEQ ID NO: 127), XP 002284244 Vitis (SEQ ID NO: 79), EOA14646 Capsella (SEQ ID NO: 61), NP 199892 AtSW10 (SEQ ID NO: 16), XP 002321731 Populus (SEQ ID NO: 82), XP 002322281 Populus (SEQ ID NO: 83), XP 002321730 Populus (SEQ ID NO: 81), XP 002511127 Ricinus (SEQ ID NO: 93), XP 002511128 Ricinus (SEQ ID NO: 94), CBI32263 Vitis (SEQ ID NO: 46), EMJ01437 Prunus (SEQ ID NO: 48), XP 002520679 Ricinus (SEQ ID NO: 96), XP 004247459 Solanum (SEQ ID NO: 125), EOA28959 Capsella (SEQ ID NO: 67), NP 181439 AtSW09 (SEQ ID NO: 15), XP 002333315 Populus (SEQ ID NO: 84), NEC1 PETHY Petunia (SEQ ID NO: 35), XP 002267792 Vitis (SEQ ID NO: 78), XP 004138978 Cucumis (SEQ ID NO: 111), XP 004138979 Cucumis (SEQ ID NO: 112), XP 003518628 Glycine (SEQ ID NO: 98), XP 004489106 Cicer (SEQ ID NO: 130), XP 003617528 Medicago (SEQ ID NO: 27), XP 004302124 Fragaria (SEQ ID NO: 129), NOD3 MEDTR Medicago (SEQ ID NO: 31), NP 001239695 Glycine (SEQ ID NO: 74), AFK39311 Lotus (SEQ ID NO: 37), XP 003620983 Medicago (SEQ ID NO: 32), XP 003530901 Glycine (SEQ ID NO: 101), XP 003524088 Glycine (SEQ ID NO: 100), XP 003615405 Medicago (SEQ ID NO: 33), XP 003547573 Glycine (SEQ ID NO: 102)].

[0061] FIG. 2-14 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-11 [XP 003593107 Medicago (SEQ ID NO: 34), EOA22072 Capsella (SEQ ID NO: 65), NP 196821 AtSW15 (SEQ ID NO: 21), EMJ23678 Prunus (SEQ ID NO: 50), XP 004301046 Fragaria (SEQ ID NO: 128), XP 002299333 Populus (SEQ ID NO: 80), XP 002514863 Ricinus (SEQ ID NO: 95), XP 004140547 Cucumis (SEQ ID NO: 113), XP 002264875 Vitis (SEQ ID NO: 77), NP 199893 AtSW13 (SEQ ID NO: 19), XP 002862913 Arabiopsis (SEQ ID NO: 97), EOA14916 Capsella (SEQ ID NO: 62), EOA17919 Capsella (SEQ ID NO: 63), NP 194231 AtSW14 (SEQ ID NO: 20), EOA21276 Capsella (SEQ ID NO: 64), NP 197755 AtSW12 (SEQ ID NO: 18), EOA24501 Capsella (SEQ ID NO: 66), NP 190443 AtSW11 (SEQ ID NO: 17), XP 002511126 Ricinus (SEQ ID NO: 92), XP 004297511 Fragaria (SEQ ID NO: 126), XP 004153501 Cucumis (SEQ ID NO: 115), XP 004161952 Cucumis (SEQ ID NO: 116), XP 004145146 Cucumis (SEQ ID NO: 114), XP 004138250 Cucumis (SEQ ID NO: 110), XP 004235470 Solanum (SEQ ID NO: 123), CBI15715 Vitis (SEQ ID NO: 45), AFW71563 Zea (SEQ ID NO: 39), NP 001149028 Zea (SEQ ID NO: 72), XP 002453892 Sorghum (SEQ ID NO: 89), EMT09236 Aegilops (SEQ ID NO: 54), XP 003575028 Brachypodium (SEQ ID NO: 105), NP 001046944 OsSW15 (SEQ ID NO: 26), EMS46194 Triticum (SEQ ID NO: 52), AFW88409 Zea (SEQ ID NO: 40), XP 002465280 Sorghum (SEQ ID NO: 91), BAJ99068 Hordeum (SEQ ID NO: 43), EMT31030 Aegilops (SEQ ID NO: 59), XP 003561640 Brachypodium (SEQ ID NO: 103), NP 001050099 OsSW12 (SEQ ID NO: 23), BAK07340 Hordeum (SEQ ID NO: 44), EMS45810 Triticum (SEQ ID NO: 51), XP 003578398 Brachypodium (SEQ ID NO: 108), XP 002462642 Sorghum (SEQ ID NO: 90), EAZ09693 Oryza (SEQ ID NO: 47), NP 001148964 Zea (SEQ ID NO: 71), XP 002444688 Sorghum (SEQ ID NO: 87), XP 003572455 Brachypodium (SEQ ID NO: 104), NP 001062354 OsSW11 (SEQ ID NO: 22), EMT31640 Aegilops (SEQ ID NO: 60), EMS51422 Triticum (SEQ ID NO: 53), EMT20808 Aegilops (SEQ ID NO: 58)].

[0062] FIG. 2-15 is a diagram illustrating a result of multiple alignment analysis of the protein contained in the phylogenetic tree illustrated in FIG. 1-1, and following the right of FIG. 2-12 [BAJ85621 Hordeum (SEQ ID NO: 41), EMT11081 Aegilops (SEQ ID NO: 55), XP 002442119 Sorghum (SEQ ID NO: 85), XP 002443167 Sorghum (SEQ ID NO: 86), NP 001141654 Zea (SEQ ID NO: 70), NP 001141106 Zea (SEQ ID NO: 69), SWT13 ORYSJ OsSW13 Oryza (SEQ ID NO: 24), XP 003576225 Brachypodium (SEQ ID NO: 107), BAJ94651 Hordeum (SEQ ID NO: 42), XP 003576036 Brachypodium (SEQ ID NO: 106), EMT20480 Aegilops (SEQ ID NO: 56), EMT20481 Aegilops (SEQ ID NO: 57), NP 001132836 Zea (SEQ ID NO: 68), XP 002450786 Sorghum (SEQ ID NO: 88), NP 001067955 OsSW14 (SEQ ID NO: 25)].

[0063] FIG. 3-1 is a diagram illustrating a result of multiple alignment analysis of the amino acid sequences of the SWEET proteins classified in the clade III in Non-Patent Literature 1 (Nature (2010) 468, 527-532) [AtSW09 (SEQ ID NO: 15), PhNEC1 (SEQ ID NO: 35), XP 003617528 Medicago (SEQ ID NO: 27), NOD3 MEDTR Medicago (SEQ ID NO: 31), XP 003620983 Medicago (SEQ ID NO: 32), AtSW15 (SEQ ID NO: 21), XP 003615405 Medicago (SEQ ID NO: 33), XP 003593107 Medicago (SEQ ID NO: 34), AtSW11 (SEQ ID NO: 17), AtSW12 (SEQ ID NO: 18), AtSW13 (SEQ ID NO: 19), AtSW14 (SEQ ID NO: 20), OsSW13 (SEQ ID NO: 24), OsSW14 (SEQ ID NO: 25), OsSW15 (SEQ ID NO: 26), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 003602780 Medicago (SEQ ID NO: 28), AtSW10 (SEQ ID NO: 16), OsSW12 (SEQ ID NO: 23), OsSW11 (SEQ ID NO: 22)].

[0064] FIG. 3-2 is a diagram illustrating a result of multiple alignment analysis of the amino acid sequences of the SWEET proteins classified in the clade III in Non-Patent Literature 1 (Nature (2010) 468, 527-532), and following below FIG. 3-1 [AtSW09 (SEQ ID NO: 15), PhNEC1 (SEQ ID NO: 35), XP 003617528 Medicago (SEQ ID NO: 27), NOD3 MEDTR Medicago (SEQ ID NO: 31), XP 003620983 Medicago (SEQ ID NO: 32), AtSW15 (SEQ ID NO: 21), XP 003615405 Medicago (SEQ ID NO: 33), XP 003593107 Medicago (SEQ ID NO: 34), AtSW11 (SEQ ID NO: 17), AtSW12 (SEQ ID NO: 18), AtSW13 (SEQ ID NO: 19), AtSW14 (SEQ ID NO: 20), OsSW13 (SEQ ID NO: 24), OsSW14 (SEQ ID NO: 25), OsSW15 (SEQ ID NO: 26), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 003602780 Medicago (SEQ ID NO: 28), AtSW10 (SEQ ID NO: 16), OsSW12 (SEQ ID NO: 23), OsSW11 (SEQ ID NO: 22)].

[0065] FIG. 3-3 is a diagram illustrating a result of multiple alignment analysis of the amino acid sequences of the SWEET proteins classified in the clade III in Non-Patent Literature 1 (Nature (2010) 468, 527-532), and following below FIG. 3-2 [AtSW09 (SEQ ID NO: 15), PhNEC1 (SEQ ID NO: 35), XP 003617528 Medicago (SEQ ID NO: 27), NOD3 MEDTR Medicago (SEQ ID NO: 31), XP 003620983 Medicago (SEQ ID NO: 32), AtSW15 (SEQ ID NO: 21), XP 003615405 Medicago (SEQ ID NO: 33), XP 003593107 Medicago (SEQ ID NO: 34), AtSW11 (SEQ ID NO: 17), AtSW12 (SEQ ID NO: 18), AtSW13 (SEQ ID NO: 19), AtSW14 (SEQ ID NO: 20), OsSW13 (SEQ ID NO: 24), OsSW14 (SEQ ID NO: 25), OsSW15 (SEQ ID NO: 26), AFK35161 Medicago (SEQ ID NO: 29), CAC44123 Medicago (SEQ ID NO: 30), XP 003602780 Medicago (SEQ ID NO: 28), AtSW10 (SEQ ID NO: 16), OsSW12 (SEQ ID NO: 23), OsSW11 (SEQ ID NO: 22)].

[0066] FIG. 4-1 is a diagram illustrating a result of multiple alignment analysis of SWEET proteins derived from Arabidopsis thaliana and SWEET proteins derived from Oryza sativa in the clade III [At5g50790.1 AtSW10 (SEQ ID NO: 16), At5g13170.1 AtSW15 (SEQ ID NO: 21), At3g48740.1 AtSW11 (SEQ ID NO: 17), At5g23660.1 AtSW12 (SEQ ID NO: 18), At5g50800.1 AtSW13 (SEQ ID NO: 19), At4g25010.1 AtSW14 (SEQ ID NO: 20), 0512g0476200 OsSW13 (SEQ ID NO: 24), 0511g0508600 OsSW14 (SEQ ID NO: 25), 0502g0513100 OsSW15 (SEQ ID NO: 26), At2g39060.1 AtSW09 (SEQ ID NO: 15), 0503g0347500 OsSW12 (SEQ ID NO: 23), 0508g0535200 OsSW11 (SEQ ID NO: 22)].

[0067] FIG. 4-2 is a diagram illustrating a result of multiple alignment analysis of SWEET proteins derived from Arabidopsis thaliana and SWEET proteins derived from Oryza sativa in the clade III, and following below FIG. 4-1 [At5g50790.1 AtSW10 (SEQ ID NO: 16), At5g13170.1 AtSW15 (SEQ ID NO: 21), At3g48740.1 AtSW11 (SEQ ID NO: 17), At5g23660.1 AtSW12 (SEQ ID NO: 18), At5g50800.1 AtSW13 (SEQ ID NO: 19), At4g25010.1 AtSW14 (SEQ ID NO: 20), 0512g0476200 OsSW13 (SEQ ID NO: 24), 0511g0508600 OsSW14 (SEQ ID NO: 25), 0502g0513100 OsSW15 (SEQ ID NO: 26), At2g39060.1 AtSW09 (SEQ ID NO: 15), 0503g0347500 OsSW12 (SEQ ID NO: 23), 0508g0535200 OsSW11 (SEQ ID NO: 22)].

[0068] FIG. 5 is a diagram illustrating a result of multiple alignment analysis of SWEET proteins derived from Arabidopsis thaliana in the clade III [At5g50790.1 AtSW10 (SEQ ID NO: 16), At5g13170.1 AtSW15 (SEQ ID NO: 21), At3g48740.1 AtSW11 (SEQ ID NO: 17), At5g23660.1 AtSW12 (SEQ ID NO: 18), At5g50800.1 AtSW13 (SEQ ID NO: 19), At4g25010.1 AtSW14 (SEQ ID NO: 20), At2g39060.1 AtSW09 (SEQ ID NO: 15)].

[0069] FIG. 6 is a configuration diagram schematically illustrating a physical map of the nucleic acid AtSWEET/pRI201AN prepared in Examples.

[0070] FIG. 7 is a photograph of the part producing guttation in Arabidopsis under conditions described in Examples.

[0071] FIG. 8 is a configuration diagram schematically illustrating a physical map of the nucleic acids pZH2B_GWOx_AtSWEET11 and pZH2B_GWOx_AtSWEET12 prepared in Examples.

[0072] FIG. 9 is a photograph of the part producing guttation under conditions described in Examples in rice.

DESCRIPTION OF EMBODIMENTS

[0073] The present invention will be described in detail below. The present invention involves introduction of a nucleic acid encoding a particular transporter protein involved in sugar transportation and/or enhancement of expression of the protein. In this way, exudates with high sugar concentrations can be collected from transformed plants in which the nucleic acid is introduced into cells and/or the expression of the protein is enhanced. As used herein, the exudate refers to a liquid oozed out of tissue in plant, including, for example, root exudate, seed exudate, guttation-liquid oozed out of the hydathode. The phenomenon in which a liquid is oozed out of the hydathode is referred to as guttation. Therefore, guttation-liquid is synonymous with guttation. In particular, the transformed plant in which a nucleic acid encoding a particular transporter protein involved in sugar transportation is introduced into cells and/or the expression of the protein is enhanced can produce guttation with high sugar concentrations.

[0074] As used herein, the meaning of nucleic acid includes naturally occurring nucleic acids such as DNA and RNA, artificial nucleic acids such as peptide nucleic acid (PNA) and nucleic acid molecules in which a base, sugar, or phosphodiester moiety is chemically modified. The meaning of the nucleic acid encoding a particular transporter protein involved in sugar transportation includes both of the gene in the genome and the transcription product of the gene.

[0075] As used herein, the sugar refers to a substance represented by the chemical formula C.sub.n(H.sub.2O).sub.m, including polysaccharides, oligosaccharides, disaccharides, and monosaccharides, including aldehyde and ketone derivatives of polyol and derivatives and condensation products related thereto. Glucosides in which aglycone such as alcohol, phenol, saponin, or pigment is bound to reduced group of sugar are also included. The monosaccharides may be classified into triose, tetrose, hexose, or pentose based on the number of carbon atoms and they may be classified into aldose, which has an aldehyde group, ketose, which has a ketone group, or the like based on a functional group in the molecule. The sugar may be divided into D-form and L-form according to the conformation at the asymmetric carbon most apart from the aldehyde or ketone group. Specific examples of the monosaccharides include glucose, fructose, galactose, mannose, xylose, xylulose, ribose, erythrose, threose, erythrulose, glyceraldehyde, dihydroxyacetone, etc. and specific examples of the disaccharides include sucrose (saccharose), lactose, maltose, trehalose, cellobiose, etc.

[0076] The plants according to the present invention have significantly increased amounts of sugar contained in exudate such as guttation in comparison with the wild type by introducing a nucleic acid encoding a particular transporter protein involved in sugar transportation into cells and/or enhancing expression of the protein. The protein may be expressed at the all cells in the plant tissue or it may be expressed in at least a part of the cells in the plant tissue. As used herein, the meaning of the plant tissue includes the plant organs such as leaf, stem, seed, root, and flower. In the present invention, introducing a nucleic acid means significantly increasing the molecular number per cell of the nucleic acid encoding a transporter protein in comparison with the molecular number in the wild type. In the present invention, enhancing expression of a transporter protein means increasing the expression of its transcription product and/or its translation product by modifying an expression regulatory region of a nucleic acid encoding the transporter protein and/or injecting the nucleic acid itself into a cell.

Transporter Protein Gene Involved in Sugar Transportation

[0077] The aforementioned "nucleic acid encoding a particular transporter protein involved in sugar transportation" encodes a transporter protein having a consensus sequence 1 comprising the following amino acid sequence:

TABLE-US-00004 [SEQ ID NO: 1] (L/I/V/M/F)x(G/A)xx(I/L/V/M/F)xxxx(L/I/V/F)(A/S) (P/S) [SEQ ID NO: 2] (1-3 aa)(P/S/T/A)T(F/L)xx(I/V)xxxKxxxxxxxxPYxxx (L/I)xxxx(L/I)x(I/L/M/V/F)xY(A/S/G) [SEQ ID NO: 3] (7-13 aa)(I/L/V/M)(1-2aa)(I/V)Nxxxxxx(E/Q)xxYxxx (Y/F)xx(Y/F)(A/G/S) [SEQ ID NO: 4] (35-36 aa)(R/Q/H)xxxxGx(V/I/L)xxxxx(V/M/L/I/F)xxxx (A/S/T)P(L/M)x(I/V)(I/M/V/L) [SEQ ID NO: 5] (2-7 aa)(V/I)(V/I/M)x(T/S)x(S/N)xx(F/Y)(M/L)(P/S) (F/I/V/L)xLSxx(L/I)(T/V)xx(A/G)xxW(F/L)xYGxxxxDxx (V/I)xxPNxxGxx(F/L)(G/S)xxQ(M/I)x(L/M/I/V/F)(Y/ H/F)

and being involved in sugar transportation.

[0078] In the amino acid sequence above, x denotes an arbitrary amino acid residue. In the amino acid sequence, the notations with 2 numbers connected by - and the following "aa" indicate that there is a sequence of arbitrary amino acids at the position and that the sequence consists of a number of amino acid residues, where the number is in the range between the 2 numbers. In the amino acid sequence, the notations with plural amino acids separated by/in a parenthesis indicate that there is one of the plural amino acids at the position. This way of notation is adopted in the description of the amino acid sequences herein.

[0079] The amino acid sequence shown above can be in other words an amino acid sequence in which the amino acid sequence set forth in SEQ ID NO: 1, 1 to 3 arbitrary amino acid residues, the amino acid sequence set forth in SEQ ID NO: 2, 7 to 13 arbitrary amino acid residues, the amino acid sequence set forth in SEQ ID NO: 3, any amino acid residue of I/L/V/M, 1 to 2 amino acid residues, the amino acid sequence set forth in SEQ ID NO: 4, 2 to 7 amino acid residues, and the amino acid sequence set forth in SEQ ID NO: 5 are connected in this order from the N-terminus to the C-terminus.

[0080] Supplementary FIG. 8 in Nature (2010) 468, 527-534 discloses a phylogenetic tree of SWEETs, transporter proteins involved in sugar transportation, based on the amino acid sequences. The document discloses SWEET proteins from thale cress (Arabidopsis thaliana), SWEET proteins from rice (Oryza sativa), SWEET proteins from bur clover (Medicago truncatula), SWEET proteins from Chlamydomonas reinhardtii, SWEET proteins from Physcomitrella patens, SWEET proteins from Petunia hybrida, SWEET proteins from Caenorhabditis elegans, and SWEET proteins from mammals. According to this phylogenetic tree, it is understood that SWEETs, transporter proteins involved in sugar transportation, are classified into five clades of I to V based on the similarity of the amino acid sequence.

[0081] Table 1 below shows corresponding GenBank ID numbers, indexes of the protein coding regions calculated from the genome data (Index in the Genome), gene names, protein names, abbreviations of the proteins, SWEET protein clade numbers, and species of the organisms of origin of SWEET proteins from Arabidopsis thaliana, SWEET proteins from Oryza sativa, and Medicago truncatula SWEET proteins and a Petunia hybrida SWEET protein among the transporter proteins SWEETs involved in sugar transportation disclosed in the document.

TABLE-US-00005 TABLE 1 Abbreviation GenBank GenBank Index in the Encoded of Encoded (NCBI) ID No. #1 (NCBI) ID No. #2 Genome Gene Name Protein Protein SWEET Clade Organism NP_564140 SWET1_ARATH At1g21460 AtSWEET1 AtSWEET1 AtSW01 I Arabidopsis thaliana NP_566493 SWET2_ARATH At3g14770 AtSWEET2 AtSWEET2 AtSW02 I Arabidopsis thaliana NP_200131 SWET3_ARATH At5g53190 AtSWEET3 AtSWEET3 AtSW03 I Arabidopsis thaliana NP_566829 SWET4_ARATH At3g28007 AtSWEET4 AtSWEET4 AtSW04 II Arabidopsis thaliana NP_201091 SWET5_ARATH At5g62850 AtSWEET5 AtSWEET5 AtSW05 II Arabidopsis thaliana NP_176849 SWET6_ARATH At1g66770 AtSWEET6 AtSWEET6 AtSW06 II Arabidopsis thaliana NP_587386 SWET7_ARATH At4g10850 AtSWEET7 AtSWEET7 AtSW07 II Arabidopsis thaliana NP_588579 SWET8_ARATH At5g40260 AtSWEET8 AtSWEET8 AtSW08 II Arabidopsis thaliana NP_181439 AAM63257 At2g39060 AtSWEET9 AtSWEET9 AtSW09 III Arabidopsis thaliana NP_199892 AED95992 At5g50790 AtSWEET10 AtSWEET10 AtSW10 III Arabidopsis thaliana NP_190443 AEE78451 At3g48740 AtSWEET11 AtSWEET11 AtSW11 III Arabidopsis thaliana NP_197755 AED93195 At5g23660 AtSWEET12 AtSWEET12 AtSW12 III Arabidopsis thaliana NP_199893 AED95993 At5g50800 AtSWEET13 AtSWEET13 AtSW13 III Arabidopsis thaliana NP_194231 AEE84991 At4g25010 AtSWEET14 AtSWEET14 AtSW14 III Arabidopsis thaliana NP_196821 AED91859 At5g13170 AtSWEET15 AtSWEET15 AtSW15 III Arabidopsis thaliana NP_188291 SWT16_ARATH At3g16690 AtSWEET16 AtSWEET16 AtSW16 IV Arabidopsis thaliana NP_193327 SWT17_ARATH At4g15920 AtSWEET17 AtSWEET17 AtSW17 IV Arabidopsis thaliana NP_001044998 SWT1A_ORYSJ Os01g0881300 OsSWEET1a OsSWEET1a OsSW01a I Oryza sativa NP_001055599 SWT1B_ORYSJ Os05g0426000 OsSWEET1b OsSWEET1b OsSW01b I Oryza sativa NP_001043270 SWT2A_ORYSJ Os01g0541800 OsSWEET2a OsSWEET2a OsSW02a I Oryza sativa NP_001043983 SWT2B_ORYSJ Os01g0700100 OsSWEET2b OsSWEET2b OsSW02b I Oryza sativa NP_001054926 SWT3A_ORYSJ Os05g0214300 OsSWEET3a OsSWEET3a OsSW03a I Oryza sativa NP_001042428 SWT3B_ORYSJ Os01g0220700 OsSWEET3b OsSWEET3b OsSW03b I Oryza sativa NP_001046621 SWET4_ORYSJ Os02g0301100 OsSWEET4 OsSWEET4 OsSW04 II Oryza sativa NP_001056475 SWET5_ORYSJ Os05g0588500 OsSWEET5 OsSWEET5 OsSW05 II Oryza sativa NP_001043523 SWT6A_ORYSJ Os01g0608000 OsSWEET6a OsSWEET6a OsSW06a II Oryza sativa NP_001043522 SWT6B_ORYSJ Os01g0805700 OsSWEET6b OsSWEET6b OsSW06b II Oryza sativa NP_001062690 SWT7A_ORYSJ Os09g0254600 OsSWEET7a OsSWEET7a OsSW07a II Oryza sativa NP_001062702 SWT7B_ORYSJ Os09g0258700 OsSWEET7b OsSWEET7b OsSW07b II Oryza sativa SWT7C_ORYSJ Os12g0178500 OsSWEET7c OsSWEET7c OsSW07c II Oryza sativa NP_001062354 -- Os08g0535200 OsSWEET11 OsSWEET11 OsSW11 III Oryza sativa NP_001050099 -- Os03g0347500 OsSWEET12 OsSWEET12 OsSW12 III Oryza sativa SWT13_ORYSJ -- Os12g0476200 OsSWEET13 OsSWEET13 OsSW13 III Oryza sativa NP_001067955 -- Os11g0508600 OsSWEET14 OsSWEET14 OsSW14 III Oryza sativa NP_001046944 -- Os02g0513100 OsSWEET15 OsSWEET15 OsSW15 III Oryza sativa NP_001050071 SWT16_ORYSJ Os03g0341300 OsSWEET16 OsSWEET16 OsSW16 IV Oryza sativa XP_003617528 -- Medtr5g092600 MtSWEET9 MtSWEET9 MtSW09 III Medicago truncatula XP_003602780 -- Medtr3g098930 MtSWEET10a MtSWEET10a MtSW10a III Medicago truncatula AFK35161 -- -- MtSWEET10b MtSWEET10b MtSW10b III Medicago truncatula CAC44123 -- -- MtSWEET10c MtSWEET10c MtSW10c III Medicago truncatula NOD3_MEDTR -- -- NOD3 MtSWEET15a MtSW15a III Medicago truncatula XP_003620983 -- Medtr7g005690 MtSWEET15b MtSWEET15b MtSW15b III Medicago truncatula XP_003615405 -- Medtr5g067530 MtSWEET15c MtSWEET15c MtSW15c III Medicago truncatula XP_003593107 -- Medtr2g007890 MtSWEET15d MtSWEET15d MtSW15d III Medicago truncatula NEC1_PETHY -- -- NEC1 PhNEC1 PhNEC1 III Petunia hybrida

[0082] As used herein, the word AtSWEET refers to AtSWEET1, AtSWEET2, AtSWEET3, AtSWEET4, AtSWEET5, AtSWEET6, AtSWEET7, AtSWEET8, AtSWEET9, AtSWEET10, AtSWEET11, AtSWEET12, AtSWEET13, AtSWEET14, AtSWEET15, AtSWEET16, and AtSWEETT17 in Table 1 and the word OsSWEET refers to OsSWEET1a, OsSWEET1b, OsSWEET2a, OsSWEET2b, OsSWEET3a, OsSWEET3b, OsSWEET4, OsSWEET5, OsSWEET6a, OsSWEET6b, OsSWEET7a, OsSWEET7b, OsSWEET7c, OsSWEET11, OsSWEET12, OsSWEET13, OsSWEET14, OsSWEET15, and OsSWEET16 in Table 1.

[0083] Consensus Sequence 1 described above is an amino acid sequence generated from a phylogenetic tree (FIG. 1-1 to FIG. 1-3) by ClustalW and multiple alignment (FIG. 2-1 to FIG. 2-15) made based on the information on amino acid sequences of SWEET proteins in the clade III defined in the aforementioned document collected from the GenBank database. Accordingly, the aforementioned transporter proteins involved in sugar transportation having Consensus Sequence 1 include the SWEET proteins classified in clade III in the aforementioned document, but no SWEET proteins classified in any of clades I, II, IV, and V in the aforementioned document. In other words, Consensus Sequence 1 described above is a sequence that is characteristic of the SWEET proteins classified in clade III in the aforementioned document and the SWEET proteins collected from the GenBank database and classified in clade III and that is a criterion for the clear distinction from those in clades I, II, IV, and V according to the aforementioned document.

[0084] FIG. 1-1 illustrates a whole picture of the phylogenetic tree and FIGS. 1-2 to 1-3 illustrate the enlargement of partial areas of the whole picture shown in FIG. 1-1. The whole picture shown in FIG. 1-1 contains neither GenBank ID nor protein names. The partial areas shown in FIGS. 1-2 to 1-3 contain GenBank IDs and protein names.

[0085] Specific examples of clade III include SWEET proteins derived from, in addition to besides thale cress (Arabidopsis thaliana), rice (Oryza sativa), bur clover (Medicago denticulata), and petunia (Petunia hybrida) listed in Table 1, soybean (Glycine max), bird's-foot trefoil (Lotus japonicus), tomato (Solanum lycopersicum), red pepper (Capsicum annuum), chick-pea (Cicer arietinum), cucumber (Cucumis sativus), peach (Prunus persica), strawberry (Fragaria vesca), grape (Vitisvinifera), Capsella rubella, poplar (Populus trichocarpa), castorbean (Ricinus communis), corn (Zea mays), sorghum (Sorghum bicolor), Tausch's goatgrass (Aegilops tauschii), purple false brome (Brachypodium distachyon), red wild einkorn (Triticumurartu), barley (Hordeum vulgare), etc., as shown in FIGS. 1-1 to 1-3.

[0086] Table 2 below shows corresponding GenBank ID numbers, gene names, species of the organisms of origin, and SEQ ID NOs of amino acid sequence of the SWEET proteins derived from Arabidopsis thaliana, Oryza sativa, Medicago denticulata, and Petunia hybrida listed in Table 1 among these SWEET proteins included in clade III.

TABLE-US-00006 TABLE 2 SEQ ID NO of GenBank ID Gene Name Species of organism of origin amino acid sequence NP_181439 AtSWEET9 Arabidopsis thaliana SEQ ID NO: 15 NP_199892 AtSWEET10 Arabidopsis thaliana SEQ ID NO: 16 NP_190443 AtSWEET11 Arabidopsis thaliana SEQ ID NO: 17 NP_197755 AtSWEET12 Arabidopsis thaliana SEQ ID NO: 18 NP_199893 AtSWEET13 Arabidopsis thaliana SEQ ID NO: 19 NP_194231 AtSWEET14 Arabidopsis thaliana SEQ ID NO: 20 NP_196821 AtSWEET15 Arabidopsis thaliana SEQ ID NO: 21 NP_001062354 OsSWEET11 Oryza sativa SEQ ID NO: 22 NP_001050099 OsSWEET12 Oryza sativa SEQ ID NO: 23 SWT13_ORYSJ OsSWEET13 Oryza sativa SEQ ID NO: 24 NP_001067955 OsSWEET14 Oryza sativa SEQ ID NO: 25 NP_003602780 OsSWEET15 Oryza sative SEQ ID NO: 26 XP_003617528 MtSWEET9 Medicago truncatula SEQ ID NO: 27 XP_003602780 MtSWEET10a Medicago truncatula SEQ ID NO: 28 AFK35161 MtSWEET10b Medicago truncatula SEQ ID NO: 29 CAC44123 MtSWEET10c Medicago trancatula SEQ ID NO: 30 NOD3_MEDTR NODS Medicago truncatula SEQ ID NO: 31 XP_003620983 MtSWEET15b Medicago truncatula SEQ ID NO: 32 XP_003515405 MtSWEET15c Medicago truncatula SEQ ID NO: 33 XP_003593107 MtSWEET15d Medicago truncatula SEQ ID NO: 34 NEC1_PETHY NEC1 Petunia hydriba SEQ ID NO: 35

[0087] Tables 3, 4, and 5 below show corresponding GenBank ID numbers, species of the organisms of origin, and SEQ ID NOs of amino acid sequences of the SWEET proteins shown in FIGS. 1-1 to 1-3 derived from organisms of species other than Arabidopsis thaliana, Oryza sativa, Medicago denticulata, and Petunia hybrida.

TABLE-US-00007 TABLE 3 SEQ ID NO of GenBank ID Species of organism of origin amino acid sequence ACV71016 Capsicum annuum SEQ ID NO: 36 AFK39311 Lotus japonicus SEQ ID NO: 37 AFK48645 Lotus japonicus SEQ ID NO: 38 AFW71563 Zea mays SEQ ID NO: 39 ARW88409 Zea mays SEQ ID NO: 40 BAJ65621 Hordeum vulgare SEQ ID NO: 41 BAJ94561 Hordeum vulgare SEQ ID NO: 42 BAJ99068 Hordeum vulgare SEQ ID NO: 43 BAK07340 Hordeum vulgare SEQ ID NO: 44 CBI15715 Vitis vinifera SEQ ID NO: 45 CBI32263 Vitis vinifera SEQ ID NO: 46 EAZ09693 Oryza sativa indica SEQ ID NO: 47 EMJ01437 Prunus persica SEQ ID NO: 48 EMJ10621 Prunus persica SEQ ID NO: 49 EMJ23678 Prunus persica SEQ ID NO: 60 EMS45810 Triticum urartu SEQ ID NO: 51 EMS46194 Triticum urartu SEQ ID NO: 52 EMS51422 Triticum urartu SEQ ID NO: 53 EMT09236 Aegilops tauschii SEQ ID NO: 54 EMT11081 Aegilops tauschii SEQ ID NO: 55 EMT20480 Aegilops tauschii SEQ ID NO: 56 EMT20481 Aegilops tauschii SEQ ID NO: 57 EMT20808 Aegilops tauschii SEQ ID NO: 58 EMT31030 Aegilops tauschii SEQ ID NO: 59 EMT31640 Aegilops tauschli SEQ ID NO: 60 EOA14646 Capsella rubella SEQ ID NO: 61 EOA14916 Capsella rubella SEQ ID NO: 62 EOA17919 Capsella rubella SEQ ID NO: 63 EOA21276 Capsella rubella SEQ ID NO: 64 EOA22072 Capsella rubella SEQ ID NO: 65 EOA24501 Capsella rubella SEQ ID NO: 66 EOA28959 Capsella rubella SEQ ID NO: 67 NP_001132836 Zea mays SEQ ID NO: 68 NP_001141106 Zea mays SEQ ID NO: 69 NP_001141654 Zea mays SEQ ID NO: 70 NP_001148964 Zea mays SEQ ID NO: 71

TABLE-US-00008 TABLE 4 SEQ ID NO of GenBank ID Species of organism of origin amino acid sequence NP_001149028 Zea mays SEQ ID NO: 72 NP_001237418 Glycine max SEQ ID NO: 73 NP_001239695 Glycine max SEQ ID NO: 74 NP_001241307 Glycine max SEQ ID NO: 75 NP_001242732 Glycine max SEQ ID NO: 76 XP_002264875 Vitis vinifera SEQ ID NO: 77 XP_002267792 Vitis vinifera SEQ ID NO: 78 XP_002284244 Vitis vinifera SEQ ID NO: 79 XP_002299333 Populus trichocarpa SEQ ID NO: 80 XP_002321730 Populus trichocarpa SEQ ID NO: 81 XP_002321731 Populus trichocarpa SEQ ID NO: 82 XP_002322281 Populus trichocarpa SEQ ID NO: 83 XP_002333315 Populus trichocarpa SEQ ID NO: 84 XP_002442119 Sorghum bicolor SEQ ID NO: 85 XP_002443167 Sorghum bicolor SEQ ID NO: 86 XP_002444688 Sorghum bicolor SEQ ID NO: 87 XP_002450786 Sorghum bicolor SEQ ID NO: 88 XP_002453892 Sorghum bicolor SEQ ID NO: 89 XP_002462642 Sorghum bicolor SEQ ID NO: 90 XP_002465280 Sorghum bicolor SEQ ID NO: 91 XP_002511126 Ricinus communis SEQ ID NO: 92 XP_002511127 Ricinus communis SEQ ID NO: 93 XP_002511128 Ricinus communis SEQ ID NO: 94 XP_002514863 Ricinus communis SEQ ID NO: 95 XP_002520679 Ricinus communis SEQ ID NO: 96 XP_002862913 Arabiopsis lyrata SEQ ID NO: 97 XP_003518628 Glycine max SEQ ID NO: 98 XP_003523161 Glycine max SEQ ID NO: 99 XP_003524088 Glycine max SEQ ID NO: 100 XP_003530901 Glycine max SEQ ID NO: 101 XP_003547573 Glycine max SEQ ID NO: 102 XP_003561640 Brachypodium distachyon SEQ ID NO: 103 XP_003572455 Brachypodium distachyon SEQ ID NO: 104 XP_003575028 Brachypodium distachyon SEQ ID NO: 105 XP_003576036 Brachypodium distachyon SEQ ID NO: 106 XP_003576225 Brachypodium distachyon SEQ ID NO: 107

TABLE-US-00009 TABLE 5 SEQ ID NO of GenBank ID Species of organism of origin amino acid sequence XP_003578398 Brachypodium distachyon SEQ ID NO: 108 XP_004138032 Cucumis sativus SEQ ID NO: 109 XP_004138250 Cucumis sativus SEQ ID NO: 110 XP_004138978 Cucumis sativus SEQ ID NO: 111 XP_004138979 Cucumis sativus SEQ ID NO: 112 XP_004140547 Cucumis sativus SEQ ID NO: 113 XP_004145146 Cucumis sativus SEQ ID NO: 114 XP_004153501 Cucumis sativus SEQ ID NO: 115 XP_004161952 Cucumis sativus SEQ ID NO: 116 XP_004235326 Solanum lycopersicum SEQ ID NO: 117 XP_004235333 Solanum lycopersicum SEQ ID NO: 118 XP_004235334 Solanum lycopersicum SEQ ID NO: 119 XP_004235339 Solanum lycopersicum SEQ ID NO: 120 XP_004235340 Solanum lycopersicum SEQ ID NO: 121 XP_004235342 Solanum lycopersicum SEQ ID NO: 122 XP_004235470 Solanum lycopersicum SEQ ID NO: 123 XP_004241452 Solanum lycopersicum SEQ ID NO: 124 XP_004247459 Solanum lycopersicum SEQ ID NO: 125 XP_004297511 Fragaria vesca SEQ ID NO: 126 XP_004297512 Fragaria vesca SEQ ID NO: 127 XP_004301046 Fragaria vesca SEQ ID NO: 128 XP_004302124 Fragaria vesca SEQ ID NO: 129 XP_004489106 Cicer arietinum SEQ ID NO: 130 XP_004503778 Cicer arietinum SEQ ID NO: 131

[0088] FIGS. 2-1 to 2-15 illustrate a result of analysis of alignment of the amino acid sequences of the SWEET proteins derived from various organisms listed in Tables 2 to 5 using ClustalW multiple sequence alignment program (available in DDBJ at National Institute of Genetics). The version and various parameters used in the analysis are shown below.

ClustalW Version, 2.1



[0089] Pairwise Alignment Parameters

[0090] Alignment Type, Slow

[0091] Slow Pairwise Alignment Options

[0092] Protein Weight Matrix, Gonnet

[0093] Gap Open, 10

[0094] Gap Extension, 0.1

Multiple Sequence Alignment Parameters

[0094]

[0095] Protein Weight Matrix, Gonnet

[0096] Gap Open, 10

[0097] Gap Extension, 0.20

[0098] Gap Distances, 5

[0099] No End Gaps, no

[0100] Iteration, none

[0101] Numiter, 1

[0102] Clustering, NJ

Output Options

[0102]

[0103] Format, Aln w/numbers

[0104] Order, Aligned

[0105] The aforementioned SWEET proteins classified in clade III of the SWEET protein are found to have Consensus sequence 1 described above, as shown in FIGS. 2-1 to 2-15. The variations of amino acid residues that can occur at the certain positions in Consensus Sequence 1 shown above are based on the following reasons. It is well known that the amino acids are classified according to their side chains of similar properties (chemical properties and the physical size) as described in Reference (1) ("McKee's Biochemistry," 3rd edition, Chapter 5 Amino acid, peptide, protein, 5.1 Amino acid, Japanese Edition supervised by Atsushi Ichikawa, translation supervised by Shinnichi Fukuoka, published by Ryosuke Sone, from Kagaku-Dojin Publishing Company, inc., ISBN4-7598-0944-9). Also, it is well known that substitution process in molecular evolution occurs frequently between amino acid residues classified in a certain group while maintaining the activity of protein. Based on this idea, a score matrix (BLOSUM) for the amino acid residue substitution is proposed in FIG. 2 in References (2): Henikoff S., Henikoff J. G., Amino-acid substitution matrices from protein blocks, Proc. Natl. Acad. Sci. USA, 89, 10915-10919 (1992) and used widely. Reference (2) is based on the findings that the substitution between amino acids having side chains of similar chemical properties has a less impact on the structure and function of the whole protein. According to References (1) and (2) mentioned above, the groups of side chains of amino acids to be considered in the multiple alignment may be those based on indexes for chemical properties, the physical size, etc. These are shown as the groups of amino acids having scores of 0 or more, or preferably amino acids having 1 or more in the score matrix (BLOSUM) disclosed in References (2). Representative groups include the following 8 groups. Another sub-grouping may be the groups of amino acids having scores of 0 or more, preferably the groups of amino acids having scores of 1 or more, or more preferably the groups of amino acids having scores of 2 or more.

1) Aliphatic Hydrophobic Amino Acid Group (ILMV Group)

[0106] This group is a group of the amino acids having an aliphatic hydrophobic side chain among the neutral non-polar amino acids shown in Reference (1) mentioned above and constituted of valine (V, Val), leucine (L, Leu), isoleucine (I, Ile), and methionine (M, Met). Among the amino acids classified as neutral non-polar amino acids in Reference (1), FGACWP are not included in this "aliphatic hydrophobic amino acid group" for the following reasons. Glycine (G, Gly) and alanine (A, Ala) have weak effects of the nonpolar groups because the sizes are not larger than the methyl group. Cysteine (C, Cys) may play an important role in S-S bonding and also have a property of forming hydrogen bonding with the oxygen atom and the nitrogen atom in nature. Phenylalanine (F, Phe) and tryptophan (W, Trp) have a side chain having a high molecular weight and a strong effect of the aromatic group. Proline (P, Pro) has a strong effect of the imino acid group, and fixes the angle of the main chain of polypeptide.

2) Group Having Hydroxy Methylene Group (ST Group)

[0107] This group is a group of amino acids having a hydroxy methylene group in the side chain among the neutral polar amino acids, and constituted of serine (S, Ser) and threonine (T, Thr). Because the hydroxyl group in the side chains of S and T is a sugar-binding site, they are often important sites for a particular activity of a certain polypeptide (protein).

3) Acidic Amino Acid (DE Group)

[0108] This group is a group of amino acids having an acidic carboxyl group in the side chain, and constituted of aspartic acid (D, Asp) and glutamic acid (E, Glu).

4) Basic Amino Acid (KR Group)

[0109] This group is a group of the basic amino acids, and constituted of lysine (K, Lys) and arginine (R, Arg). These K and R are positively charged and display basic characteristics in a wide range of pH. On the other hand, histidine (H, His), which is classified as a basic amino acid, is not classified in this group because it is hardly ionized at pH 7

5) Methylene Group=Polar Group (DHN Group)

[0110] In this group, all amino acids characteristically have, as a side chain, a methylene group bound to the .alpha. carbon atom and a polar group attached to the methylene group. They are characterized by having a methylene group, which is a nonpolar group, similar in physical size, and the group is constituted of asparagine (N, Asn, the polar group is the amido group), aspartic acid (D, Asp, the polar group is the carboxyl group), and histidine (H, His, the polar group is the imidazole group).

6) Dimethylene Group=Polar Group (EKQR Group)

[0111] In this group, all amino acids characteristically have, as a side chain, a linear hydrocarbon equal to or longer than the dimethylene group bound to the .alpha. carbon atom and a polar group attached to the hydrocarbon. They are characterized by having a dimethylene group, which is a nonpolar group, similar in physical size. The group is constituted of glutamic acid (E, Glu, the polar group is the carboxyl group), lysine (K, Lys, the polar group is the amino group), glutamine (Q, Gln, the polar group is the amido group), and arginine (R, Arg, the polar groups are the imino group and the amino group).

7) Aromatic (FYW Group)

[0112] This group is a group of aromatic amino acids, which have a benzene nucleus in the side chain and characterized by chemical properties unique to aromatic groups. The group consists of phenylalanine (F, Phe), tyrosine (Y, Tyr), and tryptophan (W, Trp).

8) Cyclic & Polar (HY Group)

[0113] This group is a group of amino acids that has a ring structure and polarity in the side chain, and constituted of histidine (H, His, the ring structure and the polar group are both the imidazole group), tyrosine (Y, Tyr, the ring structure is the benzene nucleus and the polar group is the hydroxyl group).

[0114] Based on the aforementioned amino acid groups, substitution of an amino acid residue in the amino acid sequence of a protein having a certain function with an amino acid residue in the same group can be easily expected to result in a novel protein having a similar function. For example, based on the aforementioned "1) Aliphatic hydrophobic amino acid group (ILMV group)," substitution of an isoleucine residue in the amino acid sequence of a protein having a certain function with a leucine residue can be easily expected to result in a novel protein having a similar function. If there are multiple proteins having a certain function, their amino acid sequences may be expressed as a consensus sequence. Also in such a case, substitution of an amino acid residue with an amino acid residue in the same group can be easily expected to result in a novel protein having a similar function. For example, if there are multiple proteins having a certain function and an amino acid residue in the consensus sequence calculated from them is isoleucine or leucine (L/I), based on the aforementioned "1) Aliphatic hydrophobic amino acid group (ILMV group)", substitution of the isoleucine or leucine residue with a methionine or valine residue can be easily expected to result in a novel protein having a similar function.

[0115] The aforementioned "particular transporter protein involved in a sugar transportation" can be defined as a protein that has Consensus Sequence 2 consisting of an amino acid sequence in which certain amino acid residues are added at the N-terminal side and the C-terminal side of Consensus Sequence 1 described above and the variation of amino acids that can be present at certain positions are limited. The amino acid sequence of Consensus Sequence 2 is as follows.

TABLE-US-00010 [SEQ ID NO: 6] G(L/I/V/F/M)xGx(I/V/L)(I/V/L)(S/T)xxxxL(A/S)P(L/ V/I/M)(P/S/T/A)TFxx(I/V)x(K/R)xK(S/T)xxx(F/Y)x(S/ A)xPYxx(A/S/T)LxSxxLx(L/I/M/V)(Y/F)Y(A/G) [SEQ ID NO: 7] (7-9 aa)(L/I)(I/V/L)(T/S)INxx(G/A)xx(I/V/M)(E/Q) xxYxxx(F/Y)(L/I/V/F)x(Y/F)Ax(K/R/N)xxxxx(T/A) [SEQ ID NO: 8] (7-8 aa)(V/F/L/I/M)(18-19 aa)(R/Q/H)xxxxGx(I/V) xxxxx(V/I/L/M)x(V/M)F(A/V)(A/S/T)PLx(I/V)(I/M/V/L) xxV(I/V)(K/R/Q)(T/S)(K/R)S(V/A)x(F/Y)MP(F/I/L)xLS (L/F/V)xL(T/V)(L/I)xAxxW(F/L)xYG(L/F)xxxDxx(V/I) xxPNxxGxx(L/F)(G/S)xxQMx(L/V/I)(Y/F)xx(Y/F).

[0116] The amino acid sequence of Consensus Sequence 2 can be, in other words, an amino acid sequence in which the amino acid sequence set forth in SEQ ID NO: 6, 7 to 9 arbitrary amino acid residues, the amino acid sequence set forth in SEQ ID NO: 7, 7 to 8 arbitrary amino acid residues, any amino acid residue of V/F/L/I/M, 18 to 19 amino acid residues, and the amino acid sequence set forth in SEQ ID NO: 8 are connected in this order from the N-terminus to the C-terminus.

[0117] Consensus Sequence 2 is an amino acid sequence that is shared between SWEET proteins classified in clade III in the aforementioned document. More specifically, Consensus Sequence 2 is an amino acid sequence generated from multiple alignment obtained, as described above, by the ClustalW analysis of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana, the transporter proteins involved in sugar transportation derived from Oryza sativa, the transporter proteins involved in sugar transportation derived from Medicago denticulata, and the transporter proteins involved in sugar transportation derived from Petunia hybrida classified in clade III in the aforementioned document. Therefore, Consensus Sequence 2 is a sequence that is characteristic of the SWEET proteins classified in clade III in the aforementioned documents and that is a criterion for the clear distinction from those in clades I, II, IV, and V according to the aforementioned document.

[0118] FIGS. 3-1 to 3-3 illustrate a result of analysis of alignment of the amino acid sequences of the SWEET proteins classified in clade III in the aforementioned document using ClustalW multiple sequence alignment program (available in DDBJ at National Institute Genetics; the version and various parameters used in the analysis are as described above). The SWEET protein classified in clade III in the aforementioned document are found to have Consensus Sequence 2 described above, as shown in FIGS. 3-1 to 3-3.

[0119] Furthermore, the aforementioned "particular transporter protein involved in a sugar transportation" can be defined as a protein having Consensus Sequence 3 consisting of an amino acid sequence in which certain amino acid residues are added at the N-terminal side of Consensus Sequence 2 described above and the variation of amino acids that can be present at certain positions are limited. The amino acid sequence of Consensus Sequence 3 is as follows.

TABLE-US-00011 [SEQ ID NO: 9] (A/V)xxxG(I/L/V)xGN(I/L/V)(I/L/V)S(F/L)x(V/T)xL (A/S)P(V/L/I)(P/A)TFxx(I/V)x(K/R)xK(S/T)xx(G/S) (F/Y)(Q/S/E)SxPYxx(A/S/T)LxS(A/C/S)xLx(L/I/M)(Y/F) Y(A/G)xx(K/T) [SEQ ID NO: 10] (3-5 aa)(L/M/P)(L/I)(I/L/V)(T/S)INxx(G/A)xx(I/V) (E/Q)xxY(I/L)x(L/M/V/I)(F/Y)(L/I/V/F)x(Y/F)Ax(K/R) xxxxx(T/A)xx(L/M/F/V/I)(L/F/V/I)xxx(N/D)(F/V/I/L) xx(F/L)xx(I/L/V)xxxxxx(L/I/V) [SEQ ID NO: 11] (5-6 aa)(R/Q)xxxxGx(I/V)xxxx(S/A)(V/L/M)(C/S/A)VF (A/V)(A/S)P Lx(I/V)(I/M/V)xxV(I/V)(K/R/Q)(T/S)(K/ R)S(V/A)E(F/Y)MP(F/I)xLS(L/F/V)xL(T/V)(L/I)(S/N)A (V/I)xW(F/L)xYGLxx(K/N)Dxx(V/I)xxPN(V/I)xGxx(F/L) (G/S)xxQMxL(Y/F)xx(Y/F).

[0120] The amino acid sequence of Consensus Sequence 3 can be, in other words, an amino acid sequence in which the amino acid sequence set forth in SEQ ID NO: 9, 3 to 5 arbitrary amino acid residues, the amino acid sequence set forth in SEQ ID NO: 10, 5 to 6 arbitrary amino acid residues and the amino acid sequence of SEQ ID NO: 11 are connected in this order from the N-terminus to the C-terminus.

[0121] Consensus Sequence 3 is an amino acid sequence generated from multiple alignment obtained by ClustalW analysis, as described above, of the amino acid sequence of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana and the transporter proteins involved in sugar transportation from derived Oryza sativa among the SWEET proteins classified in clade III in the aforementioned document. Therefore, Consensus Sequence 3 is a sequence that is characteristic of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana and the transporter proteins involved in sugar transportation derived from Oryza sativa classified in clade III in the aforementioned document and that is a criterion for the clear distinction from those in clades I, II, IV, and V according to the aforementioned document.

[0122] FIGS. 4-1 to 4-2 illustrate a result of analysis of alignment of the amino acid sequence of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana and the transporter proteins involved in sugar transportation derived from Oryza sativa classified in clade III in the aforementioned document using ClustalW multiple sequence alignment program (available in DDBJ at National Institute Genetics; the version and various parameters used in the analysis are as described above). The transporter proteins involved in sugar transportation derived from Arabidopsis thaliana and the transporter proteins involved in sugar transportation derived from Oryza sativa classified in clade III in the aforementioned document are found to have Consensus Sequence 3 described above, as shown in FIGS. 4-1 to 4-2.

[0123] Furthermore, the aforementioned "particular transporter protein involved in a sugar transportation" can be defined as a protein having Consensus Sequence 4 consisting of an amino acid sequence in which certain amino acid residues are added at the N-terminal side and the C-terminal side of Consensus Sequence 3 described above and the variation of amino acids that can be present at certain positions are limited. The amino acid sequence of Consensus Sequence 4 is as follows.

TABLE-US-00012 [SEQ ID NO: 12] (M/L/V)xx(T/K/N/S)xxxxAxxFG(L/I/V)LGN(I/L/V)(I/V) SFxVxL(S/A)P(V/I)PTFxxIxK(K/R)K(S/T)x(E/K)(G/S)(F/ Y)(Q/E)S(I/L)PYxx(A/S)LxS(A/C)xLx(L/I/M)YY(A/G)xxK [SEQ ID NO: 13] (4-5 aa)(L/M)(L/I)(I/V)(T/S)IN(A/S/T)(F/V)(G/A)x (F/V)(I/V)(E/Q)xxY(I/L)x(L/M/I)(F/Y)(F/V/I/L)x(Y/ F)Ax(K/R)xx(R/K)xx(T/A)(L/V/M)K(V/L/M/F)(L/I/V/F) xxx(N/D)(F/V/I)xx(F/L)xx(I/L)(L/I/V/F)(L/M/V)(L/V) xx(F/L)(L/I/V) [SEQ ID NO: 14] (5-6 aa)(R/Q)x(K/S/Q)x(L/I/V)Gx(I/V)Cxxx(S/A)(V/L) (S/C/A)VF(A/V)(A/S)PLx(I/V)(M/I/V)xxV(I/V)(K/R)T (K/R)S(V/A)E(Y/F)MPFxLS(L/F)xLT(I/L)(S/N)A(V/I)xW (L/F)xYGLx(L/I)(K/N)Dxx(V/I)A(L/F/I/M)PN(V/I)(L/ I/V)Gxx(L/F)GxxQM(I/V)L(Y/F)(V/L/I/M)(V/L/I/M)(Y/ F)(K/R/Q).

[0124] The amino acid sequence of Consensus Sequence 4 can be, in other words, an amino acid sequence in which the amino acid sequence of SEQ ID NO: 12, 4 to 5 arbitrary amino acid residues, the amino acid sequence of SEQ ID NO: 13, 5 to 6 arbitrary amino acid residues, and the amino acid sequence of SEQ ID NO: 14 are connected in this order from the N-terminus to the C-terminus.

[0125] Consensus sequence 4 is an amino acid sequence generated from multiple alignment obtained by ClustalW analysis, as described above, of the amino acid sequences of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana among the SWEET proteins classified in clade III in the aforementioned document. Therefore, Consensus Sequence 4 is a sequence that is characteristic of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana classified in clade III in the aforementioned document and that is a criterion for the clear distinction from those in clades I, II, IV, and V according to the aforementioned document.

[0126] FIG. 5 illustrates a result of analysis of alignment of the amino acid sequence of the transporter proteins involved in sugar transportation derived from Arabidopsis thaliana classified in clade III in the aforementioned document using ClustalW multiple sequence alignment program (available in DDBJ at National Institute Genetics; the version and various parameters used in the analysis are as described above). The transporter proteins involved in sugar transportation derived from Arabidopsis thaliana classified in clade III in the aforementioned document are found to have Consensus Sequence 4 described above, as shown in FIG. 5.

[0127] As described in the foregoing, the "nucleic acids encoding a particular transporter protein involved in sugar transportation" that can be used in the present invention are not particularly limited, as long as they encode a particular transporter protein involved in sugar transportation having Consensus Sequence 1, 2, 3, or 4 described above. In other words, the nucleic acids are not limited to those encoding the specific SWEET proteins listed Tables 2 to 5, but include those encoding SWEET proteins derived from organisms of species other than those listed in Tables 2 to 5. For example, nucleic acids that are derived from organisms whose sequence data is not stored in databases such as GenBank and that encode transporter proteins involved in sugar transportation having Consensus Sequence 1, 2, 3, or 4 can be also used.

[0128] Specific examples of the particular transporter protein involved in a sugar transportation can include proteins comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 131, as illustrated in Tables 2 to 5. In particular, the particular transporter protein involved in a sugar transportation may be preferably a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 35 (Table 2), more preferably a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 26 (derived from Arabidopsis thaliana or Oryza sativa), or further preferably a protein comprising an amino acid sequence set forth in any of SEQ ID NOs: 15 to 21 (derived from Arabidopsis thaliana). The most preferred examples of the particular transporter protein involved in a sugar transportation are AtSWEET11 comprising the amino acid sequence set forth in SEQ ID NO: 17, AtSWEET12 comprising the amino acid sequence set forth in SEQ ID NO: 18, OsSWEET14 comprising the amino acid sequence set forth in SEQ ID NO: 25, and OsSWEET15 comprising the amino acid sequence set forth in SEQ ID NO: 26.

[0129] The "nucleic acids encoding a particular transporter protein involved in sugar transportation" that can be used in the present invention are not limited to the nucleic acids encoding the particular transporter protein involved in sugar transportation identified by a specific SEQ ID NO as described above, but any nucleic acid encoding a particular transporter protein involved in sugar transportation having Consensus Sequence 1, 2, 3, or 4 described above can be used.

[0130] The nucleic acid encoding a particular transporter protein involved in sugar transportation means that the protein encoded by the nucleic acid has the transporter activity involved in sugar transportation. The transporter activity involved in sugar transportation is an activity measured with a FRET(Forster resonance energy transfer or fluorescence resonance energy transfer) sugar sensor localized in cytoplasm or endoplasmic reticulum (ER) for sugar transport across the ER membrane, for example, those described in Methods in Non Patent Literature 1 and 2.

[0131] Whether a certain particular transporter protein involved in sugar transportation has Consensus Sequence 1, 2, 3, or 4 or whether the nucleic acid encoding the protein encodes a protein having Consensus Sequence 1, 2, 3, or 4 can be easily determined by comparing the amino acid sequence of the protein or the amino acid sequence encoded by the nucleic acid with an amino acid sequence set forth in Consensus Sequence 1, 2, 3, or 4.

[0132] Examples of the transporter proteins involved in sugar transportation, comprising an amino acid sequence different from any of the amino acid sequences set forth in SEQ ID NOs: 15 to 131, and having Consensus Sequence 1, 2, 3, or 4 may include those encoding proteins that comprise an amino acid sequence in which one or plural amino acid sequences are deleted from, substituted with, added to, or inserted into an amino acid sequence set forth in any of SEQ ID NO: 15 to 131, and that have Consensus Sequence 1, 2, 3, or 4 and transporter activity involved in sugar transportation. As used herein, the plural amino acids mean, for example, 1 to 20, preferably, 1 to 10, more preferably, 1 to 7, further preferably, 1 to 5, and most preferably, 1 to 3 amino acids. The deletion, substitution, or addition of the amino acids can be made by modifying the nucleotide sequence of nucleic acids encoding the aforementioned particular transporter protein involved in sugar transportation by a known technique in the art. A mutation can be introduced into a nucleotide sequence by a known technique such as the Kunkel method or the gapped duplex method or a method similar to those. For example, a mutation is introduced using a kit for introducing mutation using a site-directed mutagenesis method (using, for example, Mutant-K or Mutant-G (both trade names, TAKARA Bio Inc.) or a kit of the LA PCR in vitro Mutagenesis series (trade name, TAKARA Bio Inc.)). The method for introducing mutation may be a method involving use of a chemical mutagen as represented by EMS (ethyl methanesulfonic acid), 5-bromouracil, 2-aminopurine, hydroxylamine, N-methyl-N'-nitro-N-nitrosoguanidine, and other carcinogenic compounds or a method involving treatment with a radiation as represented by X-ray, alpha-, beta-, and gamma-rays, and ion beam, or ultraviolet treatment.

[0133] Examples of the transporter proteins involved in sugar transportation, comprising an amino acid sequence different from any of the amino acid sequences of SEQ ID NOs: 15 to 131 and having Consensus Sequence 1, 2, 3, or 4 may include those encoding proteins having amino acid sequences having a similarity or an identity to an amino acid sequence set forth in any of SEQ ID NOs: 15 to 131 of, for example, 70% or more, preferably 80% or more, more preferably 90% or more, or most preferably 95% or more, having Consensus Sequence 1, 2, 3, or 4 and having transporter activity involved in sugar transportation. The values of similarity and identity mean values calculated using a computer program equipped with a Basic Local Alignment Search Tool (BLAST).RTM. program with the default setting and a database storing genetic sequence information.

[0134] Furthermore, the nucleic acids encoding the transporter proteins involved in sugar transportation, comprising an amino acid sequence different from any of the amino acid sequences of SEQ ID NOs: 15 to 131, and having Consensus Sequence 1, 2, 3, or 4 can be identified by extracting nucleic acid from the plant of interest and isolating a nucleic acid that hybridizes with a nucleic acid encoding an amino acid sequence set forth in any of SEQ ID NOs: 15 to 131 under stringent conditions, when genome information of the plant is unknown. As used herein, the stringent conditions refer to conditions in which so-called specific hybrids are formed, but nonspecific hybrids are not formed. For example, the stringent conditions can include hybridization in 6.times.SSC (sodium chloride/sodium citrate) at 45.degree. C. and then washing with 0.2 to 1.times.SSC, 0.1% SDS at 50 to 65.degree. C.; or such conditions can include hybridization in 1.times.SSC at 65 to 70.degree. C. and then washing with 0.3.times.SSC at 65 to 70.degree. C. The hybridization can be carried out by a conventionally known method such as those described in J. Sambrook et al., Molecular Cloning, A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory (1989).

[0135] As described in the foregoing, a "particular transporter protein involved in a sugar transportation" that is used in the present invention was defined as a protein having Consensus Sequence 1, 2, 3, or 4. However, the "particular transporter proteins involved in a sugar transportation" that can be used in the present invention are not limited to these proteins having Consensus Sequence 1, 2, 3, or 4.

[0136] More specifically, examples of the "particular transporter protein involved in a sugar transportation" may include those encoding proteins that comprise an amino acid sequence in which one or plural amino acid sequences are deleted from, substituted with, added to, or inserted into an amino acid sequence set forth in any of SEQ ID NOs: 15 to 131 and that have transporter activity involved in sugar transportation. As used herein, the plural amino acids mean, for example, 1 to 20, preferably, 1 to 10, more preferably, 1 to 7, further preferably, 1 to 5, and most preferably, 1 to 3 amino acids. The deletion, substitution, or addition of the amino acids can be made by modifying the nucleotide sequence of nucleic acids encoding the particular transporter protein involved in sugar transportation by a known technique in the art. The method of introducing a mutation into a nucleotide sequence can be selected from the methods described above as appropriate.

[0137] Examples of the "particular transporter protein involved in a sugar transportation" may include those encoding proteins having amino acid sequences having a similarity or an identity to an amino acid sequence set forth in any of SEQ ID NOs: 15 to 131 of, for example, 70% or more, preferably 80% or more, more preferably 90% or more, or most preferably 95% or more, and having transporter activity involved in sugar transportation. The values of similarity and identity can be calculated by the method described above.

[0138] Furthermore, examples of the "particular transporter protein involved in a sugar transportation" may include those encoding proteins that are encoded by nucleic acids that hybridize with a nucleic acid encoding an amino acid sequence of any of SEQ ID NOs: 15 to 131 under stringent conditions and that have transporter activity involved in sugar transportation. The stringent conditions here are the same as those described above.

[0139] The plant to which the present invention is applied can produce a high sugar concentration exudate by introducing a nucleic acid encoding a "particular transporter protein involved in sugar transportation" as defined above into a cell, or enhancing the expression of the protein encoded by the nucleic acid. Examples of techniques for introducing the nucleic acid encoding this transporter involved in sugar transportation into a cell can include, for example, a technique for introducing into a cell an expression vector in which a DNA encoding the transporter involved in sugar transportation is placed to allow the expression thereof. Also, examples of a technique for enhancing the expression of the nucleic acid encoding the transporter involved in sugar transportation can include a technique for modifying a transcriptional promoter located in proximate to the DNA encoding the transporter involved in sugar transportation in a plant of interest. In particular, a technique for introducing in a cell in the plant of interest an expression vector in which a DNA encoding the aforementioned transporter involved in sugar transportation is placed under the control of a promoter enabling constant expression to allow the expression thereof is preferred.

Artificial Gene Encoding Transporter Involved in Sugar Transportation

[0140] The aforementioned "nucleic acids encoding a particular transporter protein involved in a sugar transportation" are not limited to nucleic acids having a nucleotide sequence same as that of a naturally occurring nucleic acid, as long as they are nucleic acids having Consensus Sequence 1, 2, 3, or 4 and encoding a transporter involved in sugar transportation, and they may be nucleic acids having a nucleotide sequence designed artificially, i.e., artificial genes. As used herein, the artificial gene means a deoxyribonucleic acid (DNA) encoding an amino acid sequence designed artificially, and having a nucleotide sequence that does not occur naturally. The artificial gene may be a gene encoding a protein in which a part of a naturally occurring protein is modified (subjected to deletion, substitution, insertion, or the like of one or more amino acid residues), a gene encoding a chimeric protein in which naturally occurring amino acid sequences are connected, or a gene encoding a protein the whole sequence of which from the N-terminus to the C-terminus is designed uniquely.

[0141] The artificial gene may be a DNA having a nucleotide sequence encoding an amino acid sequence comprising Consensus Sequence 1, 2, 3, or 4. When a transporter gene involved in sugar transportation is designed as an artificial gene, the gene is preferably designed particularly to comprise the transmembrane domain. This domain is considered to localize the transporter at a more preferred position and contribute to the transporter activity.

[0142] More specific examples of the artificial gene encoding a transporter involved in sugar transportation can include those designed to encode amino acid sequences set forth in SEQ ID NOs: 132 to 137. These amino acid sequences set forth in SEQ ID NOs: 132 to 137 comprise one of the aforementioned consensus sequences in the N-terminal side and the transmembrane domain in the C-terminal side. The protein having the amino acid sequence set forth in SEQ ID NO: 132 is referred to as SWo1, the protein having the amino acid sequence set forth in SEQ ID NO: 133 is referred to as SWo2, the protein having the amino acid sequence set forth in SEQ ID NO: 134 is referred to as SWo3, the protein having the amino acid sequence set forth in SEQ ID NO: 135 is referred to as SWo4, the protein having the amino acid sequence set forth in SEQ ID NO: 136 is referred to as SWo5, and the protein having the amino acid sequence set forth in SEQ ID NO: 137 is referred to as SWo6.

Expression Vector

[0143] The expression vector is constructed to comprise a nucleic acid having a promoter nucleotide sequence that allows constitutive expression and a nucleic acid encoding a transporter involved in sugar transportation (including both of a nucleic acid having a naturally occurring nucleotide sequence and an artificial gene, which applies to the following as well). A variety of conventionally known vectors can be used as a base vector from which the expression vector is derived. For example, a plasmid, a bacteriophage, or a cosmid can be used and selected appropriately depending on the plant cell into which the vector is introduced and the method of introduction. Specific examples can include, for example, pBR322, pBR325, pUC19, pUC119, pBluescript, pBluescriptSK, and pBI vectors. In particular, use of a binary pBI vector is preferred when the method for introducing the vector into the plant cell is a method involving use of Agrobacterium. Specific examples of the binary pIB vector can include pBIG, pBIN19, pBI101, pBI121, pBI221, etc.

[0144] The promoter is not particularly limited, as long as it is a promoter capable of allowing the expression of the nucleic acid encoding the transporter involved in sugar transportation in the plant, and a known promoter can be preferably used. Examples of such a promoter can include, for example, cauliflower mosaic virus 35S promoter (CaMV35S), various actin gene promoters, various ubiquitin gene promoters, the nopaline synthetase gene promoter, the PR1a gene promoter in tobacco, ribulose 1 in tomato, the 5-diphosphate carboxylase/oxidase small subunit gene promoter, the napin gene promoter, the oleosin gene promoter, etc. Among these, use of cauliflower mosaic virus 35S promoter, an actin gene promoter, or a ubiquitin gene promoter can be more preferred. Use of any of the aforementioned promoter allows strong expression of any nucleic acid when introduced in a plant cell.

[0145] Promoters that can be used include promoters having the function to express a nucleic acid regionspecifically in plant. Such a promoter that can be used may be any promoter conventionally known. By using such a promoter and regionspecifically introducing the aforementioned nucleic acid encoding the transporter involved in sugar transportation, the sugar content can be increased in the exudate produced from the plant organ or tissue composed of the cells into which the nucleic acid has been introduced.

[0146] The expression vector may further comprise a nucleic acid having another segment sequence in addition to the promoter and the aforementioned nucleic acid encoding the transporter involved in sugar transportation. The nucleic acid having another segment sequence is not particularly limited and examples can include a nucleic acid having a terminator nucleotide sequence, a nucleic acid having a transformant selection marker nucleotide sequence, a nucleic acid having an enhancer nucleotide sequence, a nucleic acid having a nucleotide sequence for increasing the translation efficiency, etc. Moreover, the aforementioned recombinant expression vector may have a T-DNA region. The T-DNA region can increase the efficiency of introduction of nucleic acid, especially when introducing a nucleic acid having the aforementioned nucleotide sequence in the recombination expression vector into a plant cell using Agrobacterium.

[0147] The nucleic acid having a terminator nucleotide sequence is not particularly limited as long as it has the function as a transcription termination site, and may be a known one. Specific examples of the nucleic acid that can be used include the terminator region of nopaline synthetase gene (Nos terminator), the terminator region of cauliflower mosaic virus 35S (CaMV35S terminator), etc. In particular, use of the Nos terminator may be more preferred. In the aforementioned recombinant vector, placing a terminator at an appropriate position may prevent the synthesis of needlessly long transcript after the vector is introduced into a plant cell.

[0148] Examples of the nucleic acid having a transformant selection marker nucleotide sequence that can be used include a nucleic acid containing a drug-resistance gene. Specific examples of such a drug-resistance gene can include nucleic acids containing drug-resistance genes for hygromycin, bleomycin, kanamycin, gentamicin, chloramphenicol, etc. This allows the facilitated selection of transformed plants by selecting plants growing in media containing the aforementioned antibiotics.

[0149] Examples of the nucleic acid having a nucleotide sequence for increasing the efficiency of translation can include a nucleic acid having the omega sequence derived from tobacco mosaic virus. By placing this nucleic acid having the omega sequence in the noncoding region (5' UTR) upstream of the protein coding region, the efficiency of expression of the aforementioned nucleic acid encoding a transporter involved in sugar transportation can be increased. As seen above, nucleic acids having various DNA segment sequences can be included in the aforementioned recombinant expression vector depending on its purpose.

[0150] Methods for constructing the recombinant expression vector are not particularly limited and the recombinant expression vector can be constructed by introducing the aforementioned nucleic acid having a promoter nucleotide sequence, the nucleic acid encoding the particular transporter protein involved in sugar transportation, and optionally the aforementioned nucleic acid having another DNA segment sequence into the base vector selected as appropriate in a certain order. For example, the recombinant expression vector can be constructed by ligating the nucleic acid encoding a transporter involved in sugar transportation, the nucleic acid having a promoter nucleotide sequence, and (optionally the nucleic acid having a terminator nucleotide sequence) and introducing this into the vector.

[0151] Methods for replicating (methods for producing) the aforementioned expression vector are not particularly limited and conventionally known methods can be used. Generally, Escherichia coli may be used as a host and the vector may be replicated in the host. Any preferred strain of Escherichia coli may be selected depending on the type of vector.

Transformation

[0152] The aforementioned expression vector is introduced into a plant cell of interest by a general transformation method. Methods for introducing the expression vector into (methods for transforming) a plant cell are not particularly limited and conventionally known methods suitable for the plant cell can be used. Specific examples of such methods that can be used include methods involving use of Agrobacterium and methods involving direct introduction into plant cells. Examples of the methods involving use of Agrobacterium that can be used include the methods described in Bechtold, E., Ellis, J. and Pelletier, G. (1993) In Planta, Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis plants. C.R. Acad. Sci. Paris Sci. Vie, 316, 1194-1199. or Zyprian E, Kado Cl, Agrobacterium-mediated plant transformation by novel mini-T vectors in conjunction with a high-copy vir region helper plasmid. Plant Molecular Biology, 1990, 15 (2), 245-256.

[0153] Examples of the methods for directly introducing the expression vector into a plant cell that can be used include microinjection, electroporation, the polyethyleneglycol method, the particle gun method, protoplast fusion, the calcium phosphate method, etc.

[0154] When using one of the aforementioned methods for directly introducing the nucleic acid encoding the transporter involved in sugar transportation into a plant cell, a nucleic acid containing a transcription unit necessary for the expression of the nucleic acid encoding the transporter of interest, for example, a nucleic acid having a promoter nucleotide sequence and/or a nucleic acid having a transcription terminator nucleotide sequence; and the nucleic acid encoding the transporter of interest is sufficient and the vector function is not required. Furthermore, even a nucleic acid containing no transcription unit but only the protein-coding region of the aforementioned nucleic acid encoding the transporter involved in sugar transportation is sufficient, if the nucleic acid can be integrated in a transcription unit in the host genome and express the gene of interest. Also, even when the nucleic acid is not integrated in the host genome, it is sufficient if the aforementioned nucleic acid encoding the transporter involved in sugar transportation is transcribed and/or translated in the cell.

[0155] Examples of the plant cell into which the aforementioned expression vector or a nucleic acid containing no expression vector and encoding the transporter involved in sugar transportation of interest is introduced can include cells in tissues in plant organs such as flower, leaf, and root, callus, cells in suspension culture, etc. The expression vector may be an appropriate expression vector constructed for the type of plant to be produced if necessary or a preconstructed general-purpose expression vector may be introduced into a plant cell.

[0156] The plant constituted of cells into which the expression vector is introduced is not particularly limited. This means that the concentration of sugar contained in an exudate such as guttation can be increased in any plant by introducing the aforementioned nucleic acid encoding the transporter involved in sugar transportation. Preferred examples of such a plant are phanerogam plants. Among the phanerogam plants, angiosperm plants are more preferred. Examples of such angiosperm plants include, but are not limited to, dicot and monocot plants, for example, Brassicaceae, Gramineae, Solanaceae, Leguminosae, and Salicaceae plants (see below)

[0157] Brassicaceae thale cress (Arabidopsis thaliana), Arabiopsis lyrata, rape (Brassica rapa, Brassica napus, Brassica campestris), cabbage (Brassica oleracea var. capitata), Chinese cabbage (Brassica rapa var. pekinensis), napa cabbage (Brassica rapa var. chinensis), turnip (Brassica rapa var. rapa), nozawana (Brassica rapa var. hakabura), potherb mustard (Brassica rapa var. lancinifolia), komatsuna (Brassica rapa var. peruviridis), bok Choy (Brassica rapa var. chinensis), komatsuna (Raphanus sativus), wasabi (Wasabia japonica), Capsella rubella, etc.

[0158] Chenopodiaceae: sugar beet (Beta vulgaris).

[0159] Aceraceae sugar maple (Acer saccharum):

[0160] Euphorbiaceae: castorbean (Ricinus communis):

[0161] Solanaceae: Tobacco (Nicotiana tabacum), eggplant (Solanum melongena), potato (Solaneum tuberosum), tomato (Solanum lycopersicum)), pepper (Capsicum annuum), petunia (Petunia hybrida), etc

[0162] Fabaceae: Soybean (Glycine max), pea (Pisum sativum), broad beans (Vicia faba), Japanese wisteria (Wisteria floribunda), peanut (Arachis hypogaea), bird's-foot trefoil (Lotus japonicus), kidney bean (Phaseolus vulgaris), adzuki bean (Vigna angularis), acacia (Acacia), snail clover (Medicago truncatula), chick-pea (Cicer arietinum), etc.

[0163] Compositae: chrysanthemum (Chrysanthemum morifolium), sunflower (Helianthus annuus), etc.

[0164] Arecaceae: oil palm (Elaeis guineensis, Elaeis oleifera), coconut palm (Cocos nucifera), date palm (Phoenix dactylifera), wax palm (Copernicia), eyc.

[0165] Anacardiaceae: wax tree (Rhus succedanea), cashew tree (Anacardium occidentale), Chinese lacquer tree (Toxicodendron vernicifluum), mango (Mangifera indica), pistachio (Pistacia vera), etc.

[0166] Cucurbitaceae: pumpkin (Cucurbita maxima, Cucurbita moschata, Cucurbita pepo), cucumber (Cucumis sativus), Japanese snake gourd (Trichosanthes cucumeroides), calabash (Lagenaria siceraria var. gourda), etc.

[0167] Rosaceae: almond (Amygdalus communis), rose (Rosa), strawberry (Fragaria vesca), cherry tree (Prunus), apple (Malus pumila var. domestica), peach (Prunus persica), etc.

[0168] Vitaceae: grape (Vitis vinifera)

[0169] Caryophyllaceae: carnations (Dianthus caryophyllus), etc.

[0170] Salicaceae: poplar (Populus trichocarpa, Populus nigra, Populus tremula), etc.

[0171] Poaceae: corn (Zea mays), rice (Oryza sativa), barley (Hordeum vulgare), wheat (Triticum aestivum), red wild einkorn (Triticum urartu), Tausch's goatgrass (Aegilops tauschii), purple false brome (Brachypodium distachyon), bamboo (Phyllostachys), sugarcane (Saccharum officinarum), napier grass (Pennisetum pupureum), Erianthus (Erianthus ravenae), susuki grass (Miscanthus virgatum), sorghum (Sorghum bicolor) switchgrass (Panicum), etc.

[0172] Liliaceae: tulip (Tulipa), lily (Lilium), etc.

[0173] In particular, plants that produce relatively much exudate and have high productivity of sugar and starch, such as sugarcane, corn, rice, sorghum, wheat, sugar beet, and sugar maple, are preferred. This is because exudate collected from these plants can be used as raw materials for biofuel and bioplastics, as described in detail later.

[0174] While the nucleic acid encoding the transporter involved in sugar transportation that can be used in the present invention can be isolated from a variety of plants and used, as mentioned above, the nucleic acid can be selected as appropriate depending on the class of the plant and used. Thus, when the plant cell of interest is derived from a monocot plant, the nucleic acid encoding a transporter involved in sugar transportation to be introduced can be that isolated from a monocot plant. When the plant of interest is a plant in the family Poaceae, it is particularly preferred to introduce one of the following nucleic acids encoding a transporter involved in sugar transportation derived from Oryza sativa: the nucleic acid encoding OsSWEET13 (Os12g047620001) and the nucleic acid encoding OsSWEET14 (Os11t050860001) and the nucleic acid encoding OsSWEET15 (Os02t051310001). By introducing one of the nucleic acid encoding OsSWEET13 (Os12g047620001) and the nucleic acid encoding OsSWEET14 (Os11t050860001) and the nucleic acid encoding OsSWEET15 (Os02t051310001), the amount of sugar contained in the exudate derived from Oryza sativa can be markedly increased.

[0175] Even when the plant cell of interest is derived from a monocot plant, a nucleic acid encoding a transporter involved in sugar transportation derived from a dicot plant may be introduced. When the plant cell of interest is derived from a monocot plant, it is preferred to introduce the nucleic acid encoding AtSWEET11 (At3g48740) and the nucleic acid encoding AtSWEET12 (At5g23660), among the nucleic acids encoding a transporter involved in sugar transportation derived from Arabidopsis thaliana, a dicot plant. These nucleic acid encoding AtSWEET11 (At3g48740) and nucleic acid encoding AtSWEET12 (At5g23660) can markedly increase the amount of sugar contained in the exudate, even if the plant of interest is a monocot plant such as Oryza sativa.

Other Processes, Other Methods

[0176] After the aforementioned transformation process, a selection process for selecting an appropriate transformant from plants can be conducted by a conventionally known method. The method of the selection is not particularly limited. The appropriate transformant may be selected, for example, on the basis of drug resistance such as hygromycin resistance or by growing transformants, collecting exudate from the plants, measuring sugar contained in the collected exudate, and selecting the plant whose exudate has a concentration of sugar significantly increased in comparison with the wild type. The measurement of sugar contained in the collected exudate may be conducted by a qualitative method, but not a quantitative method. For example, the measurement may be conducted by a coloration method using a test paper that colors in response to sugar.

[0177] Progeny plants can be obtained according to a usual method from transformed plants obtained by the transformation process. By selecting progeny plants maintaining a trait associated with significantly increased expression of the aforementioned nucleic acid encoding a transporter involved in sugar transportation in comparison with the wild type on the basis of the amount of sugar contained in the exudate, stable plant strains whose exudate has an increased amount of sugar due to the trait strains can be created. From such transformed plants or progeny thereof, breeding materials such as plant cells, seeds, fruits, rootstocks, calluses, tubers, cuttings, and masses can be obtained to mass-produce, from such materials, stable plant strains whose exudate has an increased amount of sugar due to the aforementioned trait.

[0178] As described in the foregoing, the concentration of sugar contained in exudate can be significantly increased in comparison with the wild type plant by introducing a nucleic acid encoding the transporter involved in the aforementioned particular sugar transportation into a cell or enhancing the expression of the nucleic acid according to the present invention. The sugar components contained in the exudate are meant to include monosaccharide such as glucose, galactose, mannose, and fructose, and disaccharides such as sucrose, lactose, and maltose. Accordingly, by introducing the nucleic acid encoding the particular transporter involved in a sugar transportation into a cell or enhancing the expression of the gene present endogenously, the concentration of one or more of sugar components such as glucose, galactose, mannose, fructose, sucrose, lactose and maltose contained in exudate can be increased. In particular, the concentrations of glucose, fructose, and sucrose in exudate can be greatly increased according to the present invention.

[0179] In particular, when collecting guttation produced from the hydathode as exudate, it is preferred to cultivate the plant in which the nucleic acid encoding the particular transporter involved in the sugar transportation is introduced into a cell or the expression of the nucleic acid is enhanced under conditions that prevent transpiration of the produced guttation. Furthermore, it is more preferred to culture the plant under conditions in which the amount of guttation production is increased. For example, the transpiration of guttation can be prevented and the amount of guttation production can be increased by cultivating the plant in a closed space under conditions at a humidity of 80% RH or more or more preferably 90% RH or more.

[0180] For example, whereas the concentration of sugar contained in guttation of the wild type Arabidopsis thaliana is about 2.0 .mu.M (the mean, monosaccharide equivalent), the sugar concentration in guttation is increased to about 98.5 to 6057.5 .mu.M in the transformed Arabidopsis thaliana in which the aforementioned particular transporter gene involved in a sugar transportation is introduced into cells. In particular, the transformed Arabidopsis thaliana in which the nucleic acid encoding AtSWEET12 (At5g23660) is introduced into cells can produce guttation containing sugar components at a higher concentration in comparison with other transformed Arabidopsis thaliana.

[0181] Moreover, the concentration of sugar in the guttation is increased to about 1074.3 to 185641.2 .mu.M in the transformant Oryza sativa in which the aforementioned nucleic acid encoding a particular transporter involved in a sugar transportation is introduced into cells, whereas the concentration of sugar in the guttation is included to about 1.3 .mu.M (mean, monosaccharide equivalent) in the wild type Oryza sativa. In particular, the transformed Oryza sativa in which the nucleic acid encoding AtSWEET11 (At3g48740) or the nucleic acid encoding OsSWEET13 (Os12g0476200) or the nucleic acid encoding SWo5 is introduced into cells can produce guttation containing sugar components at higher concentrations in comparison with other transformed Oryza sativa plants can do. Furthermore, the transformed Oryza sativa in which the nucleic acid encoding OsSWEET15 (Os02g051310001) is introduced into cells can produce guttation containing sugar components at concentrations higher than the highest concentration of sugar in the guttation in the transformed Oryza sativa in which a nucleic acid encoding another particular transporter involved in a sugar transportation is introduced into cells, and the concentration of sugar in the guttation increases to up to 450340.4 .mu.M.

[0182] As described in the foregoing, exudate with a high sugar concentration can be collected according to the present invention. The collected exudate can be used for fermentative production of alcohol and/or organic acid. Furthermore, the collected exudate can be used as a raw material for biorefinery. For example, when guttation is used as an exudate for this, the aforementioned nucleic acid encoding the particular transporter involved in a sugar transportation is introduced into cells and the guttation collected from the plant in which the expression of the nucleic acid is enhanced can be used as it is in the reaction system for alcohol fermentation and organic acid fermentation and can be used as a raw material for biorefinery. Alternatively, the guttation collected from the plant can also be used in reaction systems for alcohol fermentation and organic acid fermentation after a concentration process or a process for adding another carbon or nitrogen source.

EXAMPLES

[0183] The present invention will be described in more detail with reference to Examples below. The technical scope of the present invention is however not limited to these Examples.

1. Construction of DNA Construct for Arabidopsis Thaliana Transformation

1.1. Preparation of DNA Encoding AtSWEET Protein by PCR

1.1.1. Amplification of DNA Encoding AtSWEET Protein

[0184] The DNAs encoding the AtSWEET1, AtSWEET2, AtSWEET3, AtSWEET4, AtSWEET5, AtSWEET6, AtSWEET7, AtSWEET9, AtSWEET11, AtSWEET12, AtSWEET13, AtSWEET15 and AtSWEET17 proteins for assessment were amplified by PCR using cDNA prepared from Arabidopsis thaliana as a template. To insert the DNAs for assessment into the pRI201AN vector (Takara Bio Inc., #3264), forward primers to which Sal I restriction enzyme recognition sequence is added to the 5' end and reverse primers to which Sac I or Pst I restriction enzyme recognition sequence was added to the 3' end were designed (Table 6).

TABLE-US-00013 TABLE 6 Name of SEQ Amplified ID DNA Name of Primer Sequence NO SWEET1 sal I-SWEET1-F26mer 5'-TAATGTCGACATGAACATCGCTCACACTATCTTCGG-3' 138 sac I-SWEET1-R 5'-TATGAGCTCTTAAACTTGAAGGTCTTGCTTTCCATTAAC-3' 139 SWEET2 sal I-SWEET2-F27mer 5'-TAATGTCGACATGGATGTTTTTGCTTTCAATGCTTC-3' 140 sac I-SWEET2-R27mer 5'-TATGAGCTCTCACACGTAAGAAACAATCAAAGGCTC-3' 141 SWEET3 sal I-SWEET3-F27mer 5'-TAATGTCGACATGGGTGATAAACTTCGATTATCCATC-3' 142 sac I-SWEET3-R28mer 5'-TATGAGCTCTTAGATCGATGAGGCATTGTTAGAATTC-3' 143 SWEET4 sal I-SWEET4-F31mer 5'-TAATGTCGACATGGTTAACGCTACAGTTGCGAGAAACATTG-3' 144 sac I-SWEET4-R30mer 5'-TATGAGCTCTCAAGCTGAAACTCGTTTAGCTTGTCCAC-3' 145 SWEET5 sal I-SWEET5-F30mer 5'-TAATGTCGACATGACGGACCCCCACACCGCCCGGACGATC-3' 146 sac I-SWEET5-R31mer 5'-TATGAGCTCTCAAGCCTGGCCAAGTTCGATTCCAGCATTC-3' 147 SWEET6 sal I-SWEET6-F33mer 5'-TAATGTCGACATGGTGCATGAACAGTTGAATCTTATTCGGAAG-3' 148 sac I-SWEET6-R32mer 5'-TATGAGCTCTCAAACGCCGCTAACTCTTTTGTTTAAATATG-3' 149 SWEET7 sal I-SWEET7-F28mer 5'-TAATGTCGACATGGTGTTTGCACATTTGAACCTTCTTC-3' 150 sac I-SWEET7-R31mer 5'-TATGAGCTCTTAAACATTGTTAGGTTCTTGGTTGGTATTC-3' 151 SWEET9 sal I-SWEET9-F31mer 5'-TAATGTCGACATGTTCCTCAAGGTTCATGAAATTGCTTTTC-3' 152 sac I-SWEET9-R27mer 5'-TATGAGCTCTCACTTCATTGGCCTCACCGATCCTTC-3' 153 SWEET11 sal I-SWEET11-F29mer 5'-TAATGTCGACATGAGTCTCTTCAACACTGAAAACACATG-3' 154 sac I-SWEET11-R27mer 5'-TATGAGCTCTCATGTAGCTGCTGCGGAAGAGGACTG-3' 155 SWEET12 sal I-SWEET12-F29mer 5'-TAATGTCGACATGGCTCTCTTCGACACTCATAACACATG-3' 156 sac I-SWEET12-R29mer 5'-TATGAGCTCTCAAGTAGTTGCAGCACTGTTTCTAACTC-3' 157 SWEET13 Nde I-SWEET13-F30mer 5'-GGAATTCCATATGGCTCTAACTAACAATTTATGGGCATTTG-3' 158 sal I-SWEET13-R30mer 5'-TAATGTCGACTTAAACTTGACTTTGTTTCTGGACATCCTTG-3' 159 SWEET15 sal I-SWEET15-F30mer 5'-TAATGTCGACATGGGAGTCATGATCAATCACCATTTCCTC-3' 160 sac I-SWEET15-R27mer 5'-TATGAGCTCTCAAACGGTTTCAGGACGAGTAGCCTC-3' 161 SWEET17 sal I-SWEET17-F30mer 5'-TAATGTCGACATGGCAGAGGCAAGTTTCTATATCGGAGT-3' 162 sac I-SWEET17-R29mer 5'-TATGAGCTCTTAAGAGAGGAGAGGTTCAACACGTGATG-3' 163

[0185] And the PCR amplification was conducted using these primers and PrimeSTAR GXL DNA polymerase (TaKaRa, #R050A). The composition of the reaction solution was shown in Table 7 and the reaction conditions were shown in Table 8.

TABLE-US-00014 TABLE 7 Component (.mu.l) Template DNA (100 ng/.mu.l) 1 .mu.l 5 .times. Prime Star GXL buffer 4 .mu.l dNTP mixture (25 mM) 1.6 .mu.l Forward primer (10 ng/.mu.l) 0.4 .mu.l Reverse primer (10 ng/.mu.l) 0.4 .mu.l Prime Star GXL (1 u/.mu.l) 0.8 .mu.l Sterile water 12.6 .mu.l Total 20 .mu.l

TABLE-US-00015 TABLE 8 94.degree. C. 5 min .dwnarw. 98.degree. C. 10 sec 50.degree. C. 30 sec {close oversize parenthesis} .times.30 cycles 68.degree. C. 1 min .dwnarw. 20.degree. C.

[0186] Next, the following process was conducted to add adenine to the 5' and 3' ends in order to insert the DNA fragments obtained by the PCR amplification into the pCR2.1-TOPO.RTM. vector DNA (Invitrogen, #1(4500-01). The composition of the reaction solution was shown in Table 9. The reaction solution shown in Table 9 was reacted at 70.degree. C. for 15 minutes.

TABLE-US-00016 TABLE 9 Component PCR reaction solution 15 .mu.l 10 .times. ExTaq buffer 3 .mu.l dNTP mixture (25 mM) 2 .mu.l Ex Taq (0.5 u/.mu.l) 0.1 .mu.l Sterile water 9.9 .mu.l Total 30 .mu.l

1.1.2. Cutting Out and Purification of Amplified DNA Fragment

[0187] The DNA fragments amplified by PCR were subjected to agarose gel electrophoresis and cut out and purified using MagExtractor-PCR & Gel Clean Up Kit (TOYOBO, #NPK-601). The cutting out and purification was conducted following the manual contained in the kit.

1.1.3. Transformation with Amplified DNA Fragment

[0188] The purified amplified DNA fragments were inserted into the pCR2.1-TOPO vector using TOPO TA Cloning.RTM. (Invitrogen, #1(4500-01). The composition of the reaction solution was shown in Table 10. The reaction solution shown in Table 10 was reacted at room temperature for 5 minutes.

TABLE-US-00017 TABLE 10 Component (.mu.l) Cut out purification product 2 .mu.l (amplified SWEET sequence) Salt solution 0.5 .mu.l pCR2.1-TOPO vector 0.5 .mu.l Total 3 .mu.l

[0189] Next, transformation was performed by adding 2 .mu.l of this reaction solution to Escherichia coli DH5.alpha. competent cells (TOYOBO, #DNA-903). After leaving the cells in ice bath for 30 minutes, the cells were subjected to heat-treatment at 42.degree. C. for 30 seconds. Subsequently, the cells were rapidly cooled in ice bath. 500 .mu.l of SOC medium (Invitrogen, #15544-034) was added and the cells were cultured in suspension at 37.degree. C., 180 rpm for 1 hour. To a LB agar plate containing kanamycin at a final concentration of 50 .mu.g/ml, 40 mg/ml X-gal and 40 .mu.l of 100 mM IPTG dissolved in 40 .mu.l of DMF (N,N-dimethylformamide) were applied and then 100 to 200 .mu.l of the culture were applied. The plate was incubated at 37.degree. C. overnight and colonies were obtained on the next morning.

1.1.4. Check of Transformation by Colony PCR and Selection for Positive Clone

[0190] As a result of the transformation, many colonies were obtained. To confirm the presence or absence of the inserted DNA in the colonies, colony PCR was conducted using M13-F: 5'-GTA AAA CGA CCA GTC TTA AG-3' (SEQ ID NO: 164) and M13-R: 5'-CAG GAA ACA GCT ATG AC-3' (SEQ ID NO: 165). The composition of the reaction solution for the colony PCR was shown in Table 11 and the PCR conditions were shown in Table 12.

TABLE-US-00018 TABLE 11 Component (.mu.l) Template DNA Colony Ampritaq Gold 360 Master Mix (ABI, #4398881) 10 .mu.l Forward primer (M13-F) (10 ng/.mu.l) 0.4 .mu.l Reverse primer (M13-R) (10 ng/.mu.l) 0.4 .mu.l Sterile water 9.2 .mu.l Total 20 .mu.l

TABLE-US-00019 TABLE 12 98.degree. C. 10 min .dwnarw. 95.degree. C. 15 sec 50.degree. C. 30 sec {close oversize parenthesis} .times.30 cycles 72.degree. C. 1 min .dwnarw. 72.degree. C. 7 min .dwnarw. 20.degree. C.

1.1.5. Purification of Plasmid DNA from Positive Clone

[0191] The plasmid DNAs were purified from the clones in which the inserted DNAs were confirmed. The purification of the plasmid DNAs were conducted using QIAprep Spin Miniprep Kit (QIAGEN, #27106) following the protocol contained in the kit.

1.1.6. Sequencing of Positive Clone

[0192] PCR amplification was conducted using the plasmid DNAs obtained in 1.1.5 as templates and M13-F and M13-R primers and the nucleotide sequences of the DNA fragments were determined by the dideoxy method (the Sanger method).

1.2. Preparation of DNA Encoding AtSWEET Protein by Chemical Synthesis

[0193] The DNA encoding the AtSWEET8, AtSWEET10, AtSWEET14, and AtSWEET16 proteins were chemically synthesized in total with their nucleotide sequences designed so as to add Pst I restriction enzyme recognition sequence to the 5' end and Sal I restriction enzyme recognition sequence to the 3' end. As a result, the DNAs encoding the AtSWEET8 and AtSWEET14 proteins inserted in the pEX-A vector (Operon Biotechnologies, Inc.) and the DNAs encoding the AtSWEET10 and AtSWEET16 proteins inserted in the pCR2.1-TOPO.RTM. vector were able to be obtained.

1.3. Cutting Out of DNA Encoding AtSWEET Protein by Restriction Enzyme Reaction and Purification

[0194] In order to cut out the DNA fragments encoding the AtSWEET proteins from the plasmid DNAs obtained in 1.1.5 and 1.2, twice of restriction enzyme treatments were conducted. The combination of restriction enzymes for each DNA is shown in Table 13.

TABLE-US-00020 TABLE 13 Name of DNA First Second AtSWEET1 Sac I Sal I AtSWEET2 Sac I Sal I AtSWEET3 Sac I Sal I AtSWEET4 Sac I Sal I AtSWEET5 Sac I Sal I AtSWEET6 Sac I Sal I AtSWEET7 Sac I Sal I AtSWEET8 Nde I Sal I AtSWEET9 Sac I Sal I AtSWEET10 Sal I Sac I AtSWEET11 Sac I Sal I AtSWEET12 Sac I Sal I AtSWEET13 Nde I Sal I AtSWEET14 Nde I Sal I AtSWEET15 Sac I Sal I AtSWEET16 Sal I Xba I AtSWEET17 Sac I Sal I

1.3.1. Sac I, Nde I, or Sal I Restriction Enzyme Reaction of Amplified DNA Fragment (First Round)

[0195] The reaction solutions shown in the tables below were prepared with Sac I (TaKaRa, #1078A), Nde I (TaKaRa, #1161A) or Sal I (TaKaRa, #1080A) and reacted at 37.degree. C. overnight to digest the plasmids obtained in 1.1.5 or 1.2. The composition of the reaction solution with Sal I was shown in Table 14, the composition of the reaction solution with Nde I was shown in Table 15, and the composition of the reaction solution with Sac I was shown in Table 16.

TABLE-US-00021 TABLE 14 Component (.mu.l) Plasmid 45 .mu.l 10 .times. L buffer 10 .mu.l Sac I 1 .mu.l DW 44 .mu.l Total 100 .mu.l

TABLE-US-00022 TABLE 15 Component (.mu.l) Plasmid 45 .mu.l 10 .times. H buffer 10 .mu.l Nde I 1 .mu.l DW 44 .mu.l Total 100 .mu.l

TABLE-US-00023 TABLE 16 Component (.mu.l) Plasmid 45 .mu.l 10 .times. H buffer 10 .mu.l Sal I 1 .mu.l DW 44 .mu.l Total 100 .mu.l

1.3.2. Purification of DNA Fragment Digested in Restriction Enzyme Reaction

[0196] Next, PCI (Phenol:Chloroform:Isoamyl alcohol=24:24:1) extraction and ethanol precipitation were performed to purify DNA. An equal volume of PCI was added to the reaction solution and the mixture was stirred and centrifuge at 15000 rpm for 5 minutes. The upper layer was collected and an equal volume of chloroform was added thereto. The mixture was similarly centrifuged and the upper layer was collected. To the collected upper layer, two times volume of ethanol was added and ethanol precipitation was conducted with Pellet Paint NF Co-Precipitant (Merck, #70748). The resultant DNA was dried and then dissolved in 44 .mu.l of sterile water.

1.3.3. Sal I, Xba I, and Sac I Restriction Enzyme Reaction of Amplified DNA Fragment (Second Round)

[0197] Next, the reaction solutions shown in the tables below were prepared with Sal I (TaKaRa, #1080A), Xba I (TaKaRa, #1093A), or Sac I (TaKaRa, #1078A) and reacted at 37.degree. C. overnight to digest the plasmids obtained in 1.3.2. The composition of the reaction solution with Sal I was shown in Table 17, the composition of the reaction solution with Xba I was shown in Table 18, and the composition of the reaction solution with Sac I was shown in Table 19.

TABLE-US-00024 TABLE 17 Component (.mu.l) Pellet 10 .times. H buffer 5 .mu.l Sal I 1 .mu.l DW 44 .mu.l Total 50 .mu.l

TABLE-US-00025 TABLE 18 Component (.mu.l) Pellet 10 .times. M buffer 5 .mu.l 100 .times. BSA 0.5 .mu.l Xba I 1 .mu.l DW 43.5 .mu.l Total 50 .mu.l

TABLE-US-00026 TABLE 19 Component (.mu.l) Pellet 10 .times. L buffer 5 .mu.l Sac I 1 .mu.l DW 44 .mu.l Total 50 .mu.l

1.3.4. Purification of DNA Fragment Digested in Restriction Enzyme Reaction

[0198] The reaction solutions obtained in 1.3.3 were subjected to agarose gel electrophoresis in a similar way to the procedure of 1.1.2 and the DNAs were cut out and purified with the MagExtractor-PCR & Gel Clean up kit.

1.4. Cutting out of pRI201AN Vector in Restriction Enzyme Reaction and Purification

[0199] To ligate the pRI201AN vector with the DNA fragments encoding the AtSWEET proteins obtained in 1.3, the vector was treated with restriction enzymes in a way similar to the procedure of 1.3.

1.5. Ligation

1.5.1. Ligation Reaction

[0200] Ligation reaction was conducted to insert the DNA fragments encoding the AtSWEET proteins obtained in 1.3 into the pRI201AN vector obtained in 1.4. Ligation reaction was conducted with DNA Ligation Kit Ver.2.1 (Takara Bio, #6022) at 16.degree. C. overnight.

1.5.2. Transformation with Ligation Reaction Product

[0201] After the abovementioned ligation reaction, transformation with 2 .mu.l of the ligation reaction solution was conducted in a way similar to 1.1.3.

1.5.3. Check of Ligation Reaction by Colony PCR

[0202] Insertion of the DNAs encoding the AtSWEET proteins into the vector was confirmed by examining the length of visualized DNA fragments amplified by colony PCR in agarose gel electrophoresis.

1.5.4. Preparation of DNA Constructs Obtained by Ligation Reaction

[0203] From the colonies in which the inserted DNAs were confirmed, the plasmid DNAs were purified to obtain the clones in which the DNA fragments of interest were inserted. The plasmid DNAs were purified with QIAprep Spin Miniprep Kit (QIAGEN, #27106) following the protocol contained in the kit. FIG. 6 illustrates the physical map of the resultant DNA construct (AtSWEET/pRI201AN). In FIG. 6, LB stands for left border, RB stands for right border, TNOS stands for transcription terminator of the nopaline synthetase gene NOS derived from the Ti plasmid in Agrobacterium tumefaciens, NPTII stands for neomycin phosphotransferase II gene from Escherichia coli, Pnos stands for transcription promoter of the nopaline synthetase gene NOS derived from the Ti plasmid in Agrobacterium tumefaciens, THSP stands for transcription terminator of the heat shock protein gene HSP derived from Arabidopsis thaliana, AtSWEET stands for DNA encoding a SWEET protein derived from Arabidopsis thaliana, P35S stands for Cauliflower mosaic virus 35S transcription promoter, AtADH 5'-UTR stands for translation enhancer of the alcohol dehydrogenase gene ADH derived from Arabidopsis thaliana, ColE1 ori stands for the reproduction origin of Escherichia coli, Ri ori stands for the reproduction origin of Agrobacterium rhizogenes, respectively.

1.6.1. Preparation of DNA Encoding OsSWEET Protein by Chemical Synthesis and Construction of Construct

[0204] The DNAs encoding the OsSWEET5, OsSWEET11, OsSWEET12, OsSWEET13, OsSWEET14, and OsSWEET15 proteins, whose nucleotide sequences were newly designed in reference to the codon usage in Arabidopsis thaliana so that there will be no change in the amino acid sequence, were designed to have an Nde I restriction enzyme recognition sequence at the start codon side and a Sac I restriction enzyme recognition sequence at the stop codon side. The designed DNAs were totally chemically synthesized and inserted into the pRI201AN vector to obtain the respective DNA constructs. The DNAs were designed so that the ATG in the Nde I restriction enzyme recognition sequence (5'CATATG3') added to the 5' end coincides with the start codons of the DNAs encoding the SWEET proteins.

1.6.2. Preparation of Artificial Gene Encoding Transporter Involved in Sugar Transportation and Construct by Chemical Synthesis

[0205] Deoxyribonucleic acids (DNAs) encoding transporters involved in sugar transportation that have Consensus Sequence 1 and that have a nucleotide sequence that does not occur naturally, or 6 artificial genes of transporters involved in sugar transportation that have Consensus Sequence 1 were prepared as follows. First, SEQ ID NOs: 168, 169, 170, 171, 172, and 173 were designed respectively as nucleic acids encoding the transporters SWo1, SWo2, SWo3, SWo4, SWo5, and SWo6 having amino acid sequences set forth in SEQ ID NOs: 132 to 137. DNAs were designed so that each of them has an Nde I restriction enzyme recognition sequence at the start codon side and a Sac I restriction enzyme recognition sequence at the stop codon side of SEQ ID NOs: 168, 169, 170, 171, 172, and 173. The designed DNAs were then totally chemically synthesized and inserted into the pRI201AN vector to obtain the 6 DNA constructs. The DNAs were designed so that the ATG in the Nde I restriction enzyme recognition sequence (5'CATATG3') added to the 5' end coincides with the start codons in SEQ ID NOs: 168, 169, 170, 171, 172, and 173.

1.7. Transformation ofArabidopsis Thaliana

[0206] The vectors for plant expression prepared in 1.5 and 1.6.1 and 1.6.2 were introduced into Agrobacterium tumefaciens strain C58C1 by electroporation (Plant Molecular Biology Manual, Second Edition, B. G. Stanton and A. S. Robbert, Kluwer Acdemic Publishers 1994). Then, Agrobacterium tumefaciens in which the vectors for plant expression were each introduced was introduced into the wild type Arabidopsis thaliana ecotype Col-0 by dipping described by Clough, et al. (Steven J. Clough and Andrew F. Bent, 1998, The Plant Journal 16, 735-743) and T1 (the first generation transformant) seeds were collected. The collected T1 seeds were sown in sterile on MS agar medium (agar concentration 0.8%) containing kanamycin (50 mg/L), carbenicillin (100 mg/L) and Benlate wettable powder (10 mg/L: Sumitomo Chemical Co., Ltd.) and cultured for about 2 weeks to select transformants. The selected transformants were transplanted onto a fresh preparation of the same MS agar medium, further cultivated for about 1 week, and then transplanted in a pot containing the soil which is a 1:1 mixture of vermiculite and Soil-mix (Sakata Seed Co.) to obtain T2 (the second generation transformant) seeds.

1.8. Preparation ofArabidopsis Thaliana Guttation

[0207] T1 or T2 plants of Arabidopsis thaliana transformed with the DNAs encoding the AtSWEET, OsSWEET, SWo1, SWo2, SWo3, SWo4, SWo5, and SWo6 proteins were cultivated with 18L/6D (24 hour cycles with 18 hours of light conditions followed by 6 hours of dark conditions) at 22.degree. C. After acclimation, 1/1000 Hyponex was given to plants cultivated for 1 to 2 weeks and the plants were wrapped with a plastic wrap (Saran Wrap (R), Asahi Chemical Industry) to increase humidity to 80% or more, or preferably 90% or more so that guttation is secreted (FIG. 7). Mainly, guttation attached to the back of leaves was collected and the sugar concentration in the guttation was analyzed. T1 seeds are defined as seeds harvested after infecting the wild type Arabidopsis thaliana with Agrobacterium and cultivating the resultant, T1 plants are defined as plants which has been confirmed to have introduction of DNA into cells, for example, by screening of T1 seeds with drug or by PCR, and T2 seeds are defined as seeds harvested after cultivating T1 plants.

2. Construction of DNA Construct for Oryza Sativa Transformation

2.1. Amplification of DNA Encoding AtSWEET Protein

[0208] Using the aforementioned DNA constructs (the DNA encoding the AtSWEET8 protein and the DNA encoding the AtSWEET11 protein and the DNA encoding the AtSWEET12 protein) for Arabidopsis thaliana transformation prepared in 1.5.4 as templates, the DNA encoding the AtSWEET8 protein and the DNA encoding the AtSWEET11 protein and the DNA encoding the AtSWEET12 protein were amplified by PCR. The sequence CACC was added to the 5' end of each amplification product for the introduction of the amplification product into the pENTR/D-TOPO vector.

2.2. Transformation with Amplified DNA Fragment

[0209] Parts of the resultant reaction solutions were subjected to agarose gel electrophoresis to confirm the presence of expected sizes of amplified products. The amplified products were then introduced into the pENTR/D-TOPO.RTM. vector using pENTER Directional TOPO Cloning Kit.RTM. (Invitrogen).

[0210] Next, Escherichia coli DH5.alpha. competent cells (Takara Bio) were transformed by adding the total amount of the reaction solutions. The cells were allowed to stand in ice bath for 30 minutes and then subjected to 45 seconds of heat treatment at 42.degree. C. Subsequently, the cells were rapidly cooled in ice bath and 300 .mu.l of SOC medium (Takara Bio) was added thereto. The mixture was cultured at 37.degree. C., with shaking at 180 rpm for 1 hour and this liquid culture was plated onto an LB agar plate containing kanamycin at a final concentration of 50 .mu.g/ml and cultured at 37.degree. C. overnight to obtain colonies on the next morning.

2.3. Check of Transformation by Colony PCR and Selection for Positive Clone

[0211] Insertion of the DNAs encoding the AtSWEET proteins into the vector was confirmed by examining the length of visualized DNA fragments amplified by colony PCR in agarose gel electrophoresis.

2.4. Purification of Plasmid DNA from Positive Clone

[0212] The plasmid DNAs were purified from the clones in which the inserted DNAs were able to be confirmed. The purification of the plasmid DNAs were conducted using QIAprep Spin Miniprep Kit (QIAGEN, #27106) following the protocol contained in the kit.

2.5. Sequencing of Positive Clone

[0213] Using the plasmid DNAs purified in 2.4 as templates and M13-F and M13-R primers, the DNA fragments were sequenced by a DNA sequencer (Beckman Coulter, CEQ8000).

2.6. LR Reaction and Transformation

[0214] The pENTR/D-TOPO.RTM. plasmid DNAs in which the DNA encoding the AtSWEET8 protein, the DNA encoding the AtSWEET11 protein, and the DNA encoding the AtSWEET12 protein were inserted obtained in 2.4 and a vector for Oryza sativa transformation (pZH2B_GWOx) were subjected to the Gateway LR reaction to construct the constructs for the overexpression in the plant of Oryza sativa, as shown in FIG. 8.

[0215] Next, Escherichia coli DH5.alpha. competent cells (Takara Bio) were transformed by adding the total amount of the reaction solutions. The cells were allowed to stand in ice bath for 30 minutes and then subjected to 45 seconds of heat treatment at 42.degree. C. Subsequently, the cells were rapidly cooled in ice bath and 300 .mu.l of SOC medium (Takara Bio) was added thereto. The mixture was cultured at 37.degree. C., with shaking at 180 rpm for 1 hour. This liquid culture was plated onto an LB agar plate containing spectionmycin at a final concentration of 50 .mu.g/ml and cultured at 37.degree. C. overnight to obtain colonies on the next morning.

2.7. Check of Transformation by Colony PCR and Selection for Positive Clone

[0216] Insertion of the DNAs encoding the AtSWEET proteins into the vector was confirmed by examining the length of visualized DNA fragments amplified by colony PCR in agarose gel electrophoresis.

2.8. Purification of Plasmid DNA from Positive Clone

[0217] The plasmid DNAs were purified from the clones in which the inserted DNAs were able to be confirmed. The plasmid DNAs were purified with QIAprep Spin Miniprep Kit (QIAGEN, #27106) following the protocol contained in the kit.

2.9. Sequencing of Positive Clone

[0218] Using the plasmid DNAs purified in 2.8 as templates and the following primers, the DNA fragments were sequenced by the DNA sequencer (Beckman Coulter, CEQ8000).

TABLE-US-00027 Ubi3'F: (SEQ ID NO: 166) 5'-TGC TGT ACT TGC TTG GTA TTG-3' UbiTseq3: (SEQ ID NO: 167) 5'-GGA CCA GAC CAG ACA ACC-3'

2.10.1. Preparation of DNA Encoding OsSWEET by Chemical Synthesis

[0219] DNAs encoding the OsSWEET13, OsSWEET14, or OsSWEET15 protein were designed to have the sequence CACC at the 5' end for the introduction into the pENTR/D-TOPO vector. The designed DNAs were totally chemically synthesized and inserted into the pENTR/D-TOPO vector.

2.10.2. Preparation of Artificial Gene Encoding Transporter Involved in Sugar Transportation by Chemical Synthesis

[0220] Deoxyribonucleic acids (DNAs) encoding transporters involved in sugar transportation that have Consensus Sequence 1 and that have a nucleotide sequence that does not occur naturally, or 2 artificial genes of transporters involved in sugar transportation that have Consensus Sequence 1 were prepared as follows. First, SEQ ID NOs: 174 and 175 were designed as nucleic acids encoding the transporters SWo1 and SWo5 having amino acid sequences set forth in SEQ ID NOs: 132 and 136. DNAs were designed to have the sequence CACC at the 5' end of SEQ ID NOs: 174 and 175 for the introduction into the pENTR/D-TOPO vector. The designed DNAs were totally chemically synthesized and inserted into the pENTR/D-TOPO vector.

2.11. Construction of Construct of DNA or Artificial Gene Encoding OsSWEET Protein

[0221] Vectors for Oryza sativa transformation were constructed using the DNAs synthesized in 2.10.1 and 2.10.2 in a way similar to 2.6 to 2.9 above.

2.12. Transformation of Oryza Sativa

[0222] The DNAs encoding the AtSWEET, OsSWEET, SWo1, and SWo5 proteins were introduced into Oryza sativa (c.v. Nipponbare) using the aforementioned vectors for plant expression constructed in 2.9 and 2.11 according to the method described in The Plant Journal (2006) 47, 969-976.

2.13. Preparation of Oryza Sativa Guttation

[0223] T1 transformants of Oryza sativa in which DNA encoding the AtSWEET, OsSWEET, SWo1, and SWo5 proteins were introduced were transplanted to a pot with a diameter of 6 cm containing 0.8 times volume of vermiculite and acclimated. Oryza sativa was cultivated with 18L (30.degree. C)/6D(25.degree. C.) (24 hours photocycle conditions with 18 hours light conditions at 30.degree. C. followed by 6 hours of dark conditions at 25.degree. C.). After acclimation, 1/1000 Hyponex was sufficiently given to plants cultivated for 1 to 2 weeks and the plants were wrapped with a plastic wrap (Saran Wrap (R), Asahi Chemical Industry) to increase humidity to 80% or more, or preferably 90% or more so that guttation is secreted from the hydathode in Oryza sativa (FIG. 9). Guttation attached to leaves was collected and analyzed for the sugar concentration.

3. Analysis for Sugar Concentration in Guttation

3.1. Dilution of Guttation Sample

[0224] The volumes of guttation from Arabidopsis thaliana obtained in 1.8 and guttation from Oryza sativa obtained in 2.13 were measured using a pipetter and pure water was added to a fixed volume of 0.35 ml. Next, the guttation was centrifuged at 10000.times.G for 10 minutes and then 0.3 mL of the supernatant was transferred to an automatic sampler vial and used for an HPLC analysis.

3.2. Analysis for Sugar Concentration by HPLC

[0225] The sugar concentration was analyzed using HPLC in the following conditions. In this analysis, a standard solution containing a mixture of glucose, fructose, and sucrose at 50 .mu.M each as standard substances was used.

[0226] Analytic column: CarboPac PA1 (Dionex)

[0227] Eluent: 100 mM NaOH

[0228] Flow rate: 1 ml/min

[0229] Amount of injection: 25 .mu.l

[0230] Detector: Pulsed amperometric detector (Dionex ED40)

4. Result of Analysis

[0231] The results of measurement of sugar concentrations in guttation from Arabidopsis thaliana obtained in 1.8 and guttation from Oryza sativa obtained in 2.13 are shown in Tables 20 and 21.

TABLE-US-00028 TABLE 20 Glc (.mu.M) Fru (.mu.M) Clade Transgene Host Ave Max Min Ave Max Min I AtSW01 A. thaliana 1.3 14.2 0.0 1.8 11.8 0.0 I AtSW02 A. thaliana 5.7 33.6 0.0 0.0 0.0 0.0 I AtSW03 A. thaliana 4.0 14.7 0.0 0.9 6.0 0.0 II AtSW04 A. thaliana 3.0 9.1 0.0 8.5 20.7 0.0 II AtSW05 A. thaliana 5.5 15.7 0.0 3.4 20.5 0.0 II AtSW06 A. thaliana 3.3 10.3 0.0 0.1 2.0 0.0 II AtSW07 A. thaliana 4.9 15.1 0.0 8.0 19.0 0.0 II AtSW08 A. thaliana 419.9 838.6 50.2 610.6 1,154.3 145.6 II AtSW08 O. sativa 571.4 1,205.6 152.4 419.0 845.5 153.1 III AtSW09 A. thaliana 399.5 2,708.3 36.4 552.5 2,838.7 69.5 III AtSW10 A. thaliana 331.6 586.3 77.0 650.9 1,085.9 215.8 III AtSW11 A. thaliana 711.1 2,137.9 80.1 674.6 1,384.7 117.6 III AtSW11 O. sativa 31,304.7 59,730.0 757.3 36,772.0 74,830.9 964.0 III AtSW12 A. thaliana 1,375.5 2,920.7 183.4 1,720.7 3,542.4 201.4 III AtSW12 O. sativa 14,006.2 45,976.5 1,081.6 11,477.3 43,830.5 1,690.7 III AtSW13 A. thaliana 230.5 941.5 51.1 304.3 1,336.8 85.5 III AtSW14 A. thaliana 60.4 211.6 24.9 163.2 451.8 74.8 III AtSW15 A. thaliana 796.6 2,064.2 143.1 1,140.0 2,727.5 226.1 IV AtSW16 A. thaliana 3.1 14.6 0.0 0.5 3.0 0.0 IV AtSW17 A. thaliana 2.0 3.5 0.0 1.2 3.7 0.0 Total Monosacharide Suc (.mu.M) Equivalent (.mu.M) Clade Transgene Ave Max Min Ave Max Min I AtSW01 0.0 0.0 0.0 3.1 22.1 0.0 I AtSW02 0.1 1.8 0.0 5.8 33.6 0.0 I AtSW03 0.2 3.4 0.0 5.2 19.6 0.0 II AtSW04 0.0 0.0 0.0 11.6 23.2 0.0 II AtSW05 0.0 0.0 0.0 8.8 30.8 0.0 II AtSW06 0.2 5.0 0.0 3.9 10.3 0.0 II AtSW07 1.6 4.9 0.0 16.1 36.9 0.0 II AtSW08 697.1 1,172.5 217.6 2,424.8 4,337.8 631.0 II AtSW08 41.9 47.8 33.6 1,074.3 2,146.7 394.3 III AtSW09 228.3 1,309.4 41.2 1,408.5 7,865.4 211.8 III AtSW10 280.9 516.6 45.2 1,544.3 2,705.4 383.1 III AtSW11 290.7 470.4 97.2 1,967.1 4,463.5 449.6 III AtSW11 8,196.6 19,339.4 110.8 84,469.9 173,239.6 1,942.9 III AtSW12 1,480.7 6,099.3 214.7 6,057.5 18,661.6 1,185.5 III AtSW12 2,598.2 22,209.9 56.4 30,679.9 130,872.6 3,247.4 III AtSW13 146.8 402.7 51.9 828.5 3,083.7 287.3 III AtSW14 48.8 118.7 22.2 321.2 900.7 151.6 III AtSW15 514.2 1,217.2 70.1 2,965.0 6,511.9 582.7 IV AtSW16 0.0 0.0 0.0 3.5 14.6 0.0 IV AtSW17 0.0 0.0 0.0 3.2 7.1 0.0

TABLE-US-00029 TABLE 21 Glc (.mu.M) Fru (.mu.M) Clade Transgene Host Ave Max Min Ave Max Min II OsSW05 A. thaliana 2.7 5.3 0.0 3.8 12.8 0.0 III OsSW11 A. thaliana 318.0 607.1 81.5 490.8 833.1 179.7 III OsSW12 A. thaliana 41.7 172.9 9.7 89.5 334.1 32.4 III OsSW13 A. thaliana 48.5 160.9 8.0 121.0 367.7 24.8 III OsSW13 O. sativa 62,407.2 125,776.4 3,917.0 77,858.6 156,842.0 4,650.0 III OsSW14 A. thaliana 37.5 128.5 10.7 115.6 460.4 45.5 III OsSW14 O. sativa 43,115.4 90,201.0 543.0 58,581.3 152,827.3 229.1 III OsSW15 A. thaliana 14.9 39.7 8.2 39.3 97.3 19.6 III OsSW15 O. sativa 33,018.8 246,007.1 197.8 31,135.4 197,244.2 461.9 III SWo1 A. thaliana 182.9 337.5 28.2 125.9 219.2 32.6 III SWo1 O. sativa 11,181.0 33,889.1 284.2 6,586.0 19,670.8 351.1 III SWo2 A. thaliana 81.6 128.4 30.6 103.3 146.3 26.7 III SWo3 A. thaliana 141.1 141.1 141.1 166.9 166.9 166.9 III SWo4 A. thaliana 41.9 96.3 12.7 35.5 88.2 3.8 III SWo5 A. thaliana 31.8 31.8 31.8 7.7 7.7 7.7 III SWo5 O. sativa 12,006.9 43,461.2 1,371.6 7,166.2 31,839.2 734.7 III SWo6 A. thaliana 179.1 455.3 33.5 121.1 155.1 64.6 -- none A. thaliana 1.6 8.1 0.0 0.3 7.3 0.0 -- none O. sativa 1.0 8.3 0.0 0.0 0.2 0.0 Total Monosacharide Suc (.mu.M) Equivalent (.mu.M) Clade Transgene Ave Max Min Ave Max Min II OsSW05 2.2 3.9 0.0 10.8 21.5 0.0 III OsSW11 221.0 723.7 14.0 1,250.7 2,887.6 360.7 III OsSW12 36.9 127.8 3.0 205.0 762.5 71.1 III OsSW13 41.3 93.7 19.6 252.1 716.0 71.9 III OsSW13 22,687.7 74,320.2 64.5 185,641.2 358,704.4 8,994.7 III OsSW14 51.2 118.0 19.4 255.6 824.9 95.0 III OsSW14 7,104.2 21,756.3 10.8 115,905.1 275,262.5 793.8 III OsSW15 25.5 82.0 7.2 105.3 300.9 59.8 III OsSW15 2,011.4 10,537.3 85.2 68,176.9 450,340.4 830.2 III SWo1 14.3 22.4 6.1 337.3 601.5 73.0 III SWo1 221.1 795.8 18.5 18,209.1 51,684.6 1,019.9 III SWo2 9.9 13.8 6.3 204.7 284.5 84.9 III SWo3 16.0 16.0 16.0 340.0 340.0 340.0 III SWo4 10.6 13.4 6.7 98.5 210.9 51.7 III SWo5 10.1 10.1 10.1 59.6 59.6 59.6 III SWo5 892.0 4,573.9 42.4 20,957.0 84,448.2 2,197.3 III SWo6 10.8 21.1 2.8 321.8 638.2 108.6 -- none 0.0 2.6 0.0 2.0 11.0 0.0 -- none 0.1 0.8 0.0 1.3 8.3 0.0

[0232] It was found that the concentration of sugar in guttation is greatly increased in all of Arabidopsis thaliana and Oryza sativa transformed with DNAs encoding AtSWEET9 to 15 and DNAs encoding OsSWEET13 to 15 classified in clade III among nucleic acids encoding SWEET proteins as seen from Tables 20 and 21. In particular, it was found that the sugar concentration in guttation can be more greatly increased when transformed with any of the DNA encoding AtSWEET11, the DNA encoding AtSWEET12, the DNA encoding AtSWEET15, the DNA encoding OsSWEET13, and the DNA encoding OsSWEET14. Moreover, it was found that the concentration of sugar in guttation is more increased in Oryza sativa transformed with DNAs encoding SWEET proteins classified in clade III than in Arabidopsis thaliana transformed with DNAs encoding SWEET proteins classified in clade III. In particular, it was found that the concentration of sugar in guttation is markedly increased in Oryza sativa transformed with any of the DNA encoding OsSWEET13, the DNA encoding OsSWEET14, and the DNA encoding OsSWEET15 than in Arabidopsis thaliana transformed with the same DNA.

[0233] Moreover, it was found that the concentration of sugar in guttation can be increased also in the plants in which an artificial gene of a transporter involved in sugar transportation that has Consensus Sequence 1 is introduced. The result revealed that the concentration of sugar in guttation can be increased in any plant, without limited by the host plant, by introducing a nucleic acid encoding a transporter involved in sugar transportation that has Consensus Sequence 1 and/or enhancing the expression of the protein.

[0234] Even in the wild type plants, sugar concentrations of around 50 .mu.M can be detected in guttation in some individuals. However, it was revealed that the effect of introducing the DNA encoding the SWEET proteins classified in Clade III is much higher than the highest concentration detected in the wild type plants as seen in the Examples.

Sequence CWU 1

1

175113PRTArtificial SequenceSynthetic consensus sequence 1misc_feature(1)..(1)Xaa is L, I, V, M or Fmisc_feature(2)..(2)Xaa can be any naturally occurring amino acidmisc_feature(3)..(3)Xaa is G or Amisc_feature(4)..(5)Xaa can be any naturally occurring amino acidmisc_feature(6)..(6)Xaa is I, L, V, M or Fmisc_feature(7)..(10)Xaa can be any naturally occurring amino acidmisc_feature(11)..(11)Xaa is L, I, V or Fmisc_feature(12)..(12)Xaa is A or Smisc_feature(13)..(13)Xaa is P or S 1Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa1 5 10234PRTArtificial SequenceSynthetic consensus sequence 1misc_feature(1)..(1)Xaa is P, S, T or Amisc_feature(3)..(3)Xaa is F or Lmisc_feature(4)..(5)Xaa can be any naturally occurring amino acidmisc_feature(6)..(6)Xaa is I or Vmisc_feature(7)..(9)Xaa can be any naturally occurring amino acidmisc_feature(11)..(18)Xaa can be any naturally occurring amino acidmisc_feature(21)..(23)Xaa can be any naturally occurring amino acidmisc_feature(24)..(24)Xaa is L or Imisc_feature(25)..(28)Xaa can be any naturally occurring amino acidmisc_feature(29)..(29)Xaa is L or Imisc_feature(30)..(30)Xaa can be any naturally occurring amino acidmisc_feature(31)..(31)Xaa is I, L, M, V or Fmisc_feature(32)..(32)Xaa can be any naturally occurring amino acidmisc_feature(34)..(34)Xaa is A, S or G 2Xaa Thr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Lys Xaa Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa Pro Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30Tyr Xaa320PRTArtificial SequenceSynthetic consensus sequence 1misc_feature(1)..(1)Xaa is I or Vmisc_feature(3)..(8)Xaa can be any naturally occurring amino acidmisc_feature(9)..(9)Xaa is E or Qmisc_feature(10)..(11)Xaa can be any naturally occurring amino acidmisc_feature(13)..(15)Xaa can be any naturally occurring amino acidmisc_feature(16)..(16)Xaa is Y or Fmisc_feature(17)..(18)Xaa can be any naturally occurring amino acidmisc_feature(19)..(19)Xaa is Y or Fmisc_feature(20)..(20)Xaa is A, G or S 3Xaa Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa Xaa Xaa 20424PRTArtificial SequenceSynthetic consensus sequence 1misc_feature(1)..(1)Xaa is R, Q or Hmisc_feature(2)..(5)Xaa can be any naturally occurring amino acidmisc_feature(7)..(7)Xaa can be any naturally occurring amino acidmisc_feature(8)..(8)Xaa is V, I or Lmisc_feature(9)..(13)Xaa can be any naturally occurring amino acidmisc_feature(14)..(14)Xaa is V, M, L, I or Fmisc_feature(15)..(18)Xaa can be any naturally occurring amino acidmisc_feature(19)..(19)Xaa is A, S or Tmisc_feature(21)..(21)Xaa is L or Mmisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(23)..(23)Xaa is I or Vmisc_feature(24)..(24)Xaa is I, M, V or L 4Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa1 5 10 15Xaa Xaa Xaa Pro Xaa Xaa Xaa Xaa 20555PRTArtificial SequenceSynthetic consensus sequence 1misc_feature(1)..(1)Xaa is V or Imisc_feature(2)..(2)Xaa is V, I or Mmisc_feature(3)..(3)Xaa can be any naturally occurring amino acidmisc_feature(4)..(4)Xaa is T or Smisc_feature(5)..(5)Xaa can be any naturally occurring amino acidmisc_feature(6)..(6)Xaa is S or Nmisc_feature(7)..(8)Xaa can be any naturally occurring amino acidmisc_feature(9)..(9)Xaa is F or Ymisc_feature(10)..(10)Xaa is M or Lmisc_feature(11)..(11)Xaa is P or Smisc_feature(12)..(12)Xaa is F, I, V or Lmisc_feature(13)..(13)Xaa can be any naturally occurring amino acidmisc_feature(16)..(17)Xaa can be any naturally occurring amino acidmisc_feature(18)..(18)Xaa is L or Imisc_feature(19)..(19)Xaa is T or Vmisc_feature(20)..(21)Xaa can be any naturally occurring amino acidmisc_feature(22)..(22)Xaa is A or Gmisc_feature(23)..(24)Xaa can be any naturally occurring amino acidmisc_feature(26)..(26)Xaa is F or Lmisc_feature(27)..(27)Xaa can be any naturally occurring amino acidmisc_feature(30)..(33)Xaa can be any naturally occurring amino acidmisc_feature(35)..(36)Xaa can be any naturally occurring amino acidmisc_feature(37)..(37)Xaa is V or Imisc_feature(38)..(39)Xaa can be any naturally occurring amino acidmisc_feature(42)..(43)Xaa can be any naturally occurring amino acidmisc_feature(45)..(46)Xaa can be any naturally occurring amino acidmisc_feature(47)..(47)Xaa is F or Lmisc_feature(48)..(48)Xaa is G or Smisc_feature(49)..(50)Xaa can be any naturally occurring amino acidmisc_feature(52)..(52)Xaa is M or Imisc_feature(53)..(53)Xaa can be any naturally occurring amino acidmisc_feature(54)..(54)Xaa is L, M, I, V or Fmisc_feature(55)..(55)Xaa is Y, H or F 5Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Ser Xaa1 5 10 15Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Xaa Tyr Gly Xaa Xaa Xaa 20 25 30Xaa Asp Xaa Xaa Xaa Xaa Xaa Pro Asn Xaa Xaa Gly Xaa Xaa Xaa Xaa 35 40 45Xaa Xaa Gln Xaa Xaa Xaa Xaa 50 55650PRTArtificial SequenceSynthetic consensus sequence 2misc_feature(2)..(2)Xaa is L, I, V, F or Mmisc_feature(3)..(3)Xaa can be any naturally occurring amino acidmisc_feature(5)..(5)Xaa can be any naturally occurring amino acidmisc_feature(6)..(6)Xaa is I, V or Lmisc_feature(7)..(7)Xaa is I, V or Lmisc_feature(8)..(8)Xaa is S or Tmisc_feature(9)..(12)Xaa can be any naturally occurring amino acidmisc_feature(14)..(14)Xaa is A or Smisc_feature(16)..(16)Xaa is L, V, I or Mmisc_feature(17)..(17)Xaa is P, S, T or Amisc_feature(20)..(21)Xaa can be any naturally occurring amino acidmisc_feature(22)..(22)Xaa is I or Vmisc_feature(23)..(23)Xaa can be any naturally occurring amino acidmisc_feature(24)..(24)Xaa is K or Rmisc_feature(25)..(25)Xaa can be any naturally occurring amino acidmisc_feature(27)..(27)Xaa is S or Tmisc_feature(28)..(30)Xaa can be any naturally occurring amino acidmisc_feature(31)..(31)Xaa is F or Ymisc_feature(32)..(32)Xaa can be any naturally occurring amino acidmisc_feature(33)..(33)Xaa is S or Amisc_feature(34)..(34)Xaa can be any naturally occurring amino acidmisc_feature(37)..(38)Xaa can be any naturally occurring amino acidmisc_feature(39)..(39)Xaa is A, S or Tmisc_feature(41)..(41)Xaa can be any naturally occurring amino acidmisc_feature(43)..(44)Xaa can be any naturally occurring amino acidmisc_feature(46)..(46)Xaa can be any naturally occurring amino acidmisc_feature(47)..(47)Xaa is L, I, M or Vmisc_feature(48)..(48)Xaa is Y or Fmisc_feature(50)..(50)Xaa is A or G 6Gly Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Pro Xaa1 5 10 15Xaa Thr Phe Xaa Xaa Xaa Xaa Xaa Xaa Lys Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30Xaa Xaa Pro Tyr Xaa Xaa Xaa Leu Xaa Ser Xaa Xaa Leu Xaa Xaa Xaa 35 40 45Tyr Xaa 50731PRTArtificial SequenceSynthetic consensus sequence 2misc_feature(1)..(1)Xaa is L or Imisc_feature(2)..(2)Xaa is I, V or Lmisc_feature(3)..(3)Xaa is T or Smisc_feature(6)..(7)Xaa can be any naturally occurring amino acidmisc_feature(8)..(8)Xaa is G or Amisc_feature(9)..(10)Xaa can be any naturally occurring amino acidmisc_feature(11)..(11)Xaa is I, V or Mmisc_feature(12)..(12)Xaa is E or Qmisc_feature(13)..(14)Xaa can be any naturally occurring amino acidmisc_feature(16)..(18)Xaa can be any naturally occurring amino acidmisc_feature(19)..(19)Xaa is F or Ymisc_feature(20)..(20)Xaa is L, I, V or Fmisc_feature(21)..(21)Xaa can be any naturally occurring amino acidmisc_feature(22)..(22)Xaa is Y or Fmisc_feature(24)..(24)Xaa can be any naturally occurring amino acidmisc_feature(25)..(25)Xaa is K, R or Nmisc_feature(26)..(30)Xaa can be any naturally occurring amino acidmisc_feature(31)..(31)Xaa is T or A 7Xaa Xaa Xaa Ile Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa1 5 10 15Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30884PRTArtificial SequenceSynthetic consensus sequence 2misc_feature(1)..(1)Xaa is R, Q or Hmisc_feature(2)..(5)Xaa can be any naturally occurring amino acidmisc_feature(7)..(7)Xaa can be any naturally occurring amino acidmisc_feature(8)..(8)Xaa is I or Vmisc_feature(9)..(13)Xaa can be any naturally occurring amino acidmisc_feature(14)..(14)Xaa is V, I, L or Mmisc_feature(15)..(15)Xaa can be any naturally occurring amino acidmisc_feature(16)..(16)Xaa is V or Mmisc_feature(18)..(18)Xaa is A or Vmisc_feature(19)..(19)Xaa is A, S or Tmisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(23)..(23)Xaa is I or Vmisc_feature(24)..(24)Xaa is I, M, V or Lmisc_feature(25)..(26)Xaa can be any naturally occurring amino acidmisc_feature(28)..(28)Xaa is I or Vmisc_feature(29)..(29)Xaa is K, R or Qmisc_feature(30)..(30)Xaa is T or Smisc_feature(31)..(31)Xaa is K or Rmisc_feature(33)..(33)Xaa is V or Amisc_feature(34)..(34)Xaa can be any naturally occurring amino acidmisc_feature(35)..(35)Xaa is F or Ymisc_feature(38)..(38)Xaa is F, I or Lmisc_feature(39)..(39)Xaa can be any naturally occurring amino acidmisc_feature(42)..(42)Xaa is L, F or Vmisc_feature(43)..(43)Xaa can be any naturally occurring amino acidmisc_feature(45)..(45)Xaa is T or Vmisc_feature(46)..(46)Xaa is L or Imisc_feature(47)..(47)Xaa can be any naturally occurring amino acidmisc_feature(49)..(50)Xaa can be any naturally occurring amino acidmisc_feature(52)..(52)Xaa is F or Lmisc_feature(53)..(53)Xaa can be any naturally occurring amino acidmisc_feature(56)..(56)Xaa is L or Fmisc_feature(57)..(59)Xaa can be any naturally occurring amino acidmisc_feature(61)..(62)Xaa can be any naturally occurring amino acidmisc_feature(63)..(63)Xaa is V or Imisc_feature(64)..(65)Xaa can be any naturally occurring amino acidmisc_feature(68)..(69)Xaa can be any naturally occurring amino acidmisc_feature(71)..(72)Xaa can be any naturally occurring amino acidmisc_feature(73)..(73)Xaa is L or Fmisc_feature(74)..(74)Xaa is G or Smisc_feature(75)..(76)Xaa can be any naturally occurring amino acidmisc_feature(79)..(79)Xaa can be any naturally occurring amino acidmisc_feature(80)..(80)Xaa is L, V or Imisc_feature(81)..(81)Xaa is Y or Fmisc_feature(82)..(83)Xaa can be any naturally occurring amino acidmisc_feature(84)..(84)Xaa is Y or F 8Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa1 5 10 15Phe Xaa Xaa Pro Leu Xaa Xaa Xaa Xaa Xaa Val Xaa Xaa Xaa Xaa Ser 20 25 30Xaa Xaa Xaa Met Pro Xaa Xaa Leu Ser Xaa Xaa Leu Xaa Xaa Xaa Ala 35 40 45Xaa Xaa Trp Xaa Xaa Tyr Gly Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Xaa 50 55 60Xaa Pro Asn Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Gln Met Xaa Xaa65 70 75 80Xaa Xaa Xaa Xaa957PRTArtificial SequenceSynthetic consensus sequence 3misc_feature(1)..(1)Xaa is A or Vmisc_feature(2)..(4)Xaa can be any naturally occurring amino acidmisc_feature(6)..(6)Xaa is I, L or Vmisc_feature(7)..(7)Xaa can be any naturally occurring amino acidmisc_feature(10)..(10)Xaa is I, L or Vmisc_feature(11)..(11)Xaa is I, L or Vmisc_feature(13)..(13)Xaa is F or Lmisc_feature(14)..(14)Xaa can be any naturally occurring amino acidmisc_feature(15)..(15)Xaa is V or Tmisc_feature(16)..(16)Xaa can be any naturally occurring amino acidmisc_feature(18)..(18)Xaa is A or Smisc_feature(20)..(20)Xaa is V, L or Imisc_feature(21)..(21)Xaa is P or Amisc_feature(24)..(25)Xaa can be any naturally occurring amino acidmisc_feature(26)..(26)Xaa is I or Vmisc_feature(27)..(27)Xaa can be any naturally occurring amino acidmisc_feature(28)..(28)Xaa is K or Rmisc_feature(29)..(29)Xaa can be any naturally occurring amino acidmisc_feature(31)..(31)Xaa is S or Tmisc_feature(32)..(33)Xaa can be any naturally occurring amino acidmisc_feature(34)..(34)Xaa is G or Smisc_feature(35)..(35)Xaa is F or Ymisc_feature(36)..(36)Xaa is Q, S or Emisc_feature(38)..(38)Xaa can be any naturally occurring amino acidmisc_feature(41)..(42)Xaa can be any naturally occurring amino acidmisc_feature(43)..(43)Xaa is A, S or Tmisc_feature(45)..(45)Xaa can be any naturally occurring amino acidmisc_feature(47)..(47)Xaa is A, C or Smisc_feature(48)..(48)Xaa can be any naturally occurring amino acidmisc_feature(50)..(50)Xaa can be any naturally occurring amino acidmisc_feature(51)..(51)Xaa is L, I or Mmisc_feature(52)..(52)Xaa is Y or Fmisc_feature(54)..(54)Xaa is A or Gmisc_feature(55)..(56)Xaa can be any naturally occurring amino acidmisc_feature(57)..(57)Xaa is K or T 9Xaa Xaa Xaa Xaa Gly Xaa Xaa Gly Asn Xaa Xaa Ser Xaa Xaa Xaa Xaa1 5 10 15Leu Xaa Pro Xaa Xaa Thr Phe Xaa Xaa Xaa Xaa Xaa Xaa Lys Xaa Xaa 20 25 30Xaa Xaa Xaa Xaa Ser Xaa Pro Tyr Xaa Xaa Xaa Leu Xaa Ser Xaa Xaa 35 40 45Leu Xaa Xaa Xaa Tyr Xaa Xaa Xaa Xaa 50 551054PRTArtificial SequenceSynthetic consensus sequence 3misc_feature(1)..(1)Xaa is L, M or Pmisc_feature(2)..(2)Xaa is L or Imisc_feature(3)..(3)Xaa is I, L or Vmisc_feature(4)..(4)Xaa is T or Smisc_feature(7)..(8)Xaa can be any naturally occurring amino acidmisc_feature(9)..(9)Xaa is G or Amisc_feature(10)..(11)Xaa can be any naturally occurring amino acidmisc_feature(12)..(12)Xaa is I or Vmisc_feature(13)..(13)Xaa is E or Qmisc_feature(14)..(15)Xaa can be any naturally occurring amino acidmisc_feature(17)..(17)Xaa is I or Lmisc_feature(18)..(18)Xaa can be any naturally occurring amino acidmisc_feature(19)..(19)Xaa is L, M, V or Imisc_feature(20)..(20)Xaa is F or Ymisc_feature(21)..(21)Xaa is L, I, V or Fmisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(23)..(23)Xaa is Y or Fmisc_feature(25)..(25)Xaa can be any naturally occurring amino acidmisc_feature(26)..(26)Xaa is K or Rmisc_feature(27)..(31)Xaa can be any naturally occurring amino acidmisc_feature(32)..(32)Xaa is T or Amisc_feature(33)..(34)Xaa can be any naturally occurring amino acidmisc_feature(35)..(35)Xaa is L, M, F, V or Imisc_feature(36)..(36)Xaa is L, F, V or Imisc_feature(37)..(39)Xaa can be any naturally occurring amino acidmisc_feature(40)..(40)Xaa is N or Dmisc_feature(41)..(41)Xaa is F, V, I or Lmisc_feature(42)..(43)Xaa can be any naturally occurring amino acidmisc_feature(44)..(44)Xaa is F or Lmisc_feature(45)..(46)Xaa can be any naturally occurring amino acidmisc_feature(47)..(47)Xaa is I, L or Vmisc_feature(48)..(53)Xaa can be any naturally occurring amino acidmisc_feature(54)..(54)Xaa is L, I or V 10Xaa Xaa Xaa Xaa Ile Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr1 5 10 15Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 35 40 45Xaa Xaa Xaa Xaa Xaa Xaa 501184PRTArtificial SequenceSynthetic consensus sequence 3misc_feature(1)..(1)Xaa is R or Qmisc_feature(2)..(5)Xaa can be any naturally occurring amino acidmisc_feature(7)..(7)Xaa can be any naturally occurring amino acidmisc_feature(8)..(8)Xaa is I or Vmisc_feature(9)..(12)Xaa can be any naturally occurring amino acidmisc_feature(13)..(13)Xaa is S or Amisc_feature(14)..(14)Xaa is V, L or Mmisc_feature(15)..(15)Xaa is C, S or Amisc_feature(18)..(18)Xaa is A or Vmisc_feature(19)..(19)Xaa is A or Smisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(23)..(23)Xaa is I or Vmisc_feature(24)..(24)Xaa is I, M or Vmisc_feature(25)..(26)Xaa can be any naturally occurring amino acidmisc_feature(28)..(28)Xaa is I or Vmisc_feature(29)..(29)Xaa is K, R or Qmisc_feature(30)..(30)Xaa is T or Smisc_feature(31)..(31)Xaa is K or Rmisc_feature(33)..(33)Xaa is V or Amisc_feature(35)..(35)Xaa is F or Ymisc_feature(38)..(38)Xaa is F or Imisc_feature(39)..(39)Xaa can be any naturally occurring amino acidmisc_feature(42)..(42)Xaa is L, F or Vmisc_feature(43)..(43)Xaa can be any naturally occurring amino acidmisc_feature(45)..(45)Xaa is T or Vmisc_feature(46)..(46)Xaa is L or Imisc_feature(47)..(47)Xaa is S or Nmisc_feature(49)..(49)Xaa is V or Imisc_feature(50)..(50)Xaa can be any naturally occurring amino acidmisc_feature(52)..(52)Xaa is F or Lmisc_feature(53)..(53)Xaa can be any naturally occurring amino acidmisc_feature(57)..(58)Xaa can be any naturally occurring amino acidmisc_feature(59)..(59)Xaa is K or Nmisc_feature(61)..(62)Xaa can be any naturally occurring amino acidmisc_feature(63)..(63)Xaa is V or Imisc_feature(64)..(65)Xaa can be any naturally occurring amino acidmisc_feature(68)..(68)Xaa is V or Imisc_feature(69)..(69)Xaa can be any naturally occurring amino acidmisc_feature(71)..(72)Xaa can be any naturally occurring amino acidmisc_feature(73)..(73)Xaa is F or Lmisc_feature(74)..(74)Xaa is G or Smisc_feature(75)..(76)Xaa can be any naturally occurring amino acidmisc_feature(79)..(79)Xaa can be any naturally occurring amino acidmisc_feature(81)..(81)Xaa is Y or

Fmisc_feature(82)..(83)Xaa can be any naturally occurring amino acidmisc_feature(84)..(84)Xaa is Y or F 11Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Val1 5 10 15Phe Xaa Xaa Pro Leu Xaa Xaa Xaa Xaa Xaa Val Xaa Xaa Xaa Xaa Ser 20 25 30Xaa Glu Xaa Met Pro Xaa Xaa Leu Ser Xaa Xaa Leu Xaa Xaa Xaa Ala 35 40 45Xaa Xaa Trp Xaa Xaa Tyr Gly Leu Xaa Xaa Xaa Asp Xaa Xaa Xaa Xaa 50 55 60Xaa Pro Asn Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Gln Met Xaa Leu65 70 75 80Xaa Xaa Xaa Xaa1265PRTArtificial SequenceSynthetic consensus sequence 4misc_feature(1)..(1)Xaa is M, L or Vmisc_feature(2)..(3)Xaa can be any naturally occurring amino acidmisc_feature(4)..(4)Xaa is T, K, N or Smisc_feature(5)..(8)Xaa can be any naturally occurring amino acidmisc_feature(10)..(11)Xaa can be any naturally occurring amino acidmisc_feature(14)..(14)Xaa is L, I or Vmisc_feature(18)..(18)Xaa is I, L or Vmisc_feature(19)..(19)Xaa is I or Vmisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(24)..(24)Xaa can be any naturally occurring amino acidmisc_feature(26)..(26)Xaa is S or Amisc_feature(28)..(28)Xaa is V or Imisc_feature(32)..(33)Xaa can be any naturally occurring amino acidmisc_feature(35)..(35)Xaa can be any naturally occurring amino acidmisc_feature(37)..(37)Xaa is K or Rmisc_feature(39)..(39)Xaa is S or Tmisc_feature(40)..(40)Xaa can be any naturally occurring amino acidmisc_feature(41)..(41)Xaa is E or Kmisc_feature(42)..(42)Xaa is G or Smisc_feature(43)..(43)Xaa is F or Ymisc_feature(44)..(44)Xaa is Q or Emisc_feature(46)..(46)Xaa is I or Lmisc_feature(49)..(50)Xaa can be any naturally occurring amino acidmisc_feature(51)..(51)Xaa is A or Smisc_feature(53)..(53)Xaa can be any naturally occurring amino acidmisc_feature(55)..(55)Xaa is A or Cmisc_feature(56)..(56)Xaa can be any naturally occurring amino acidmisc_feature(58)..(58)Xaa can be any naturally occurring amino acidmisc_feature(59)..(59)Xaa is L, I or Mmisc_feature(62)..(62)Xaa is A or Gmisc_feature(63)..(64)Xaa can be any naturally occurring amino acid 12Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Phe Gly Xaa Leu Gly1 5 10 15Asn Xaa Xaa Ser Phe Xaa Val Xaa Leu Xaa Pro Xaa Pro Thr Phe Xaa 20 25 30Xaa Ile Xaa Lys Xaa Lys Xaa Xaa Xaa Xaa Xaa Xaa Ser Xaa Pro Tyr 35 40 45Xaa Xaa Xaa Leu Xaa Ser Xaa Xaa Leu Xaa Xaa Tyr Tyr Xaa Xaa Xaa 50 55 60Lys651354PRTArtificial SequenceSynthetic consensus sequence 4misc_feature(1)..(1)Xaa is L or Mmisc_feature(2)..(2)Xaa is L or Imisc_feature(3)..(3)Xaa is I or Vmisc_feature(4)..(4)Xaa is T or Smisc_feature(7)..(7)Xaa is A, S or Tmisc_feature(8)..(8)Xaa is F or Vmisc_feature(9)..(9)Xaa is G or Amisc_feature(10)..(10)Xaa can be any naturally occurring amino acidmisc_feature(11)..(11)Xaa is F or Vmisc_feature(12)..(12)Xaa is I or Vmisc_feature(13)..(13)Xaa is E or Qmisc_feature(14)..(15)Xaa can be any naturally occurring amino acidmisc_feature(17)..(17)Xaa is I or Lmisc_feature(18)..(18)Xaa can be any naturally occurring amino acidmisc_feature(19)..(19)Xaa is L, M or Imisc_feature(20)..(20)Xaa is F or Ymisc_feature(21)..(21)Xaa is F, V, I or Lmisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(23)..(23)Xaa is Y or Fmisc_feature(25)..(25)Xaa can be any naturally occurring amino acidmisc_feature(26)..(26)Xaa is K or Rmisc_feature(27)..(28)Xaa can be any naturally occurring amino acidmisc_feature(29)..(29)Xaa is R or Kmisc_feature(30)..(31)Xaa can be any naturally occurring amino acidmisc_feature(32)..(32)Xaa is T or Amisc_feature(33)..(33)Xaa is L, V or Mmisc_feature(35)..(35)Xaa is V, L, M or Fmisc_feature(36)..(36)Xaa is L, I, V or Fmisc_feature(37)..(39)Xaa can be any naturally occurring amino acidmisc_feature(40)..(40)Xaa is N or Dmisc_feature(41)..(41)Xaa is F, V or Imisc_feature(42)..(43)Xaa can be any naturally occurring amino acidmisc_feature(44)..(44)Xaa is F or Lmisc_feature(45)..(46)Xaa can be any naturally occurring amino acidmisc_feature(47)..(47)Xaa is I or Lmisc_feature(48)..(48)Xaa is L, I, V or Fmisc_feature(49)..(49)Xaa is L, M or Vmisc_feature(50)..(50)Xaa is L or Vmisc_feature(51)..(52)Xaa can be any naturally occurring amino acidmisc_feature(53)..(53)Xaa is F or Lmisc_feature(54)..(54)Xaa is L, I or V 13Xaa Xaa Xaa Xaa Ile Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr1 5 10 15Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30Xaa Lys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 35 40 45Xaa Xaa Xaa Xaa Xaa Xaa 501485PRTArtificial SequenceSynthetic consensus sequence 4misc_feature(1)..(1)Xaa is R or Qmisc_feature(2)..(2)Xaa can be any naturally occurring amino acidmisc_feature(3)..(3)Xaa is K, S or Qmisc_feature(4)..(4)Xaa can be any naturally occurring amino acidmisc_feature(5)..(5)Xaa is L, I or Vmisc_feature(7)..(7)Xaa can be any naturally occurring amino acidmisc_feature(8)..(8)Xaa is I or Vmisc_feature(10)..(12)Xaa can be any naturally occurring amino acidmisc_feature(13)..(13)Xaa is S or Amisc_feature(14)..(14)Xaa is V or Lmisc_feature(15)..(15)Xaa is S, C or Amisc_feature(18)..(18)Xaa is A or Vmisc_feature(19)..(19)Xaa is A or Smisc_feature(22)..(22)Xaa can be any naturally occurring amino acidmisc_feature(23)..(23)Xaa is I or Vmisc_feature(24)..(24)Xaa is M, I or Vmisc_feature(25)..(26)Xaa can be any naturally occurring amino acidmisc_feature(28)..(28)Xaa is I or Vmisc_feature(29)..(29)Xaa is K or Rmisc_feature(31)..(31)Xaa is K or Rmisc_feature(33)..(33)Xaa is V or Amisc_feature(35)..(35)Xaa is Y or Fmisc_feature(39)..(39)Xaa can be any naturally occurring amino acidmisc_feature(42)..(42)Xaa is L or Fmisc_feature(43)..(43)Xaa can be any naturally occurring amino acidmisc_feature(46)..(46)Xaa is I or Lmisc_feature(47)..(47)Xaa is S or Nmisc_feature(49)..(49)Xaa is V or Imisc_feature(50)..(50)Xaa can be any naturally occurring amino acidmisc_feature(52)..(52)Xaa is L or Fmisc_feature(53)..(53)Xaa can be any naturally occurring amino acidmisc_feature(57)..(57)Xaa can be any naturally occurring amino acidmisc_feature(58)..(58)Xaa is L or Imisc_feature(59)..(59)Xaa is K or Nmisc_feature(61)..(62)Xaa can be any naturally occurring amino acidmisc_feature(63)..(63)Xaa is V or Imisc_feature(65)..(65)Xaa is L, F, I or Mmisc_feature(68)..(68)Xaa is V or Imisc_feature(69)..(69)Xaa is L, I or Vmisc_feature(71)..(72)Xaa can be any naturally occurring amino acidmisc_feature(73)..(73)Xaa is L or Fmisc_feature(75)..(76)Xaa can be any naturally occurring amino acidmisc_feature(79)..(79)Xaa is I or Vmisc_feature(81)..(81)Xaa is Y or Fmisc_feature(82)..(82)Xaa is V, L, I or Mmisc_feature(83)..(83)Xaa is V, I or Mmisc_feature(84)..(84)Xaa is Y or Fmisc_feature(85)..(85)Xaa is K, R or Q 14Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Val1 5 10 15Phe Xaa Xaa Pro Leu Xaa Xaa Xaa Xaa Xaa Val Xaa Xaa Thr Xaa Ser 20 25 30Xaa Glu Xaa Met Pro Phe Xaa Leu Ser Xaa Xaa Leu Thr Xaa Xaa Ala 35 40 45Xaa Xaa Trp Xaa Xaa Tyr Gly Leu Xaa Xaa Xaa Asp Xaa Xaa Xaa Ala 50 55 60Xaa Pro Asn Xaa Xaa Gly Xaa Xaa Xaa Gly Xaa Xaa Gln Met Xaa Leu65 70 75 80Xaa Xaa Xaa Xaa Xaa 8515258PRTArabidopsis thaliana 15Met Phe Leu Lys Val His Glu Ile Ala Phe Leu Phe Gly Leu Leu Gly1 5 10 15Asn Ile Val Ser Phe Gly Val Phe Leu Ser Pro Val Pro Thr Phe Tyr 20 25 30Gly Ile Tyr Lys Lys Lys Ser Ser Lys Gly Phe Gln Ser Ile Pro Tyr 35 40 45Ile Cys Ala Leu Ala Ser Ala Thr Leu Leu Leu Tyr Tyr Gly Ile Met 50 55 60Lys Thr His Ala Tyr Leu Ile Ile Ser Ile Asn Thr Phe Gly Cys Phe65 70 75 80Ile Glu Ile Ser Tyr Leu Phe Leu Tyr Ile Leu Tyr Ala Pro Arg Glu 85 90 95Ala Lys Ile Ser Thr Leu Lys Leu Ile Val Ile Cys Asn Ile Gly Gly 100 105 110Leu Gly Leu Leu Ile Leu Leu Val Asn Leu Leu Val Pro Lys Gln His 115 120 125Arg Val Ser Thr Val Gly Trp Val Cys Ala Ala Tyr Ser Leu Ala Val 130 135 140Phe Ala Ser Pro Leu Ser Val Met Arg Lys Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Phe Leu Leu Ser Leu Ser Leu Thr Leu Asn Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Ile Lys Asp Lys Phe Ile Ala 180 185 190Met Pro Asn Ile Leu Gly Phe Leu Phe Gly Val Ala Gln Met Ile Leu 195 200 205Tyr Met Met Tyr Gln Gly Ser Thr Lys Thr Asp Leu Pro Thr Glu Asn 210 215 220Gln Leu Ala Asn Lys Thr Asp Val Asn Glu Val Pro Ile Val Ala Val225 230 235 240Glu Leu Pro Asp Val Gly Ser Asp Asn Val Glu Gly Ser Val Arg Pro 245 250 255Met Lys16289PRTArabidopsis thaliana 16Met Ala Ile Ser Gln Ala Val Leu Ala Thr Val Phe Gly Ile Leu Gly1 5 10 15Asn Ile Ile Ser Phe Phe Val Cys Leu Ala Pro Ile Pro Thr Phe Val 20 25 30Arg Ile Tyr Lys Arg Lys Ser Ser Glu Gly Tyr Gln Ser Ile Pro Tyr 35 40 45Val Ile Ser Leu Phe Ser Ala Met Leu Trp Met Tyr Tyr Ala Met Ile 50 55 60Lys Lys Asp Ala Met Met Leu Ile Thr Ile Asn Ser Phe Ala Phe Val65 70 75 80Val Gln Ile Val Tyr Ile Ser Leu Phe Phe Phe Tyr Ala Pro Lys Lys 85 90 95Glu Lys Thr Leu Thr Val Lys Phe Val Leu Phe Val Asp Val Leu Gly 100 105 110Phe Gly Ala Ile Phe Val Leu Thr Tyr Phe Ile Ile His Ala Asn Lys 115 120 125Arg Val Gln Val Leu Gly Tyr Ile Cys Met Val Phe Ala Leu Ser Val 130 135 140Phe Val Ala Pro Leu Gly Ile Ile Arg Lys Val Ile Lys Thr Lys Ser145 150 155 160Ala Glu Phe Met Pro Phe Gly Leu Ser Phe Phe Leu Thr Leu Ser Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Met Asn Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Ile Phe Gly Val Leu Gln Met Ile Leu 195 200 205Phe Leu Ile Tyr Lys Lys Pro Gly Thr Lys Val Leu Glu Pro Pro Gly 210 215 220Ile Lys Leu Gln Asp Ile Ser Glu His Val Val Asp Val Val Arg Leu225 230 235 240Ser Thr Met Val Cys Asn Ser Gln Met Arg Thr Leu Val Pro Gln Asp 245 250 255Ser Ala Asp Met Glu Ala Thr Ile Asp Ile Asp Glu Lys Ile Lys Gly 260 265 270Asp Ile Glu Lys Asn Lys Asp Glu Lys Glu Val Phe Leu Ile Ser Lys 275 280 285Asn17289PRTArabidopsis thaliana 17Met Ser Leu Phe Asn Thr Glu Asn Thr Trp Ala Phe Val Phe Gly Leu1 5 10 15Leu Gly Asn Leu Ile Ser Phe Ala Val Phe Leu Ser Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Trp Lys Lys Lys Thr Thr Glu Gly Phe Gln Ser Ile 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Thr Leu Trp Leu Tyr Tyr Ala 50 55 60Thr Gln Lys Lys Asp Val Phe Leu Leu Val Thr Ile Asn Ala Phe Gly65 70 75 80Cys Phe Ile Glu Thr Ile Tyr Ile Ser Met Phe Leu Ala Tyr Ala Pro 85 90 95Lys Pro Ala Arg Met Leu Thr Val Lys Met Leu Leu Leu Met Asn Phe 100 105 110Gly Gly Phe Cys Ala Ile Leu Leu Leu Cys Gln Phe Leu Val Lys Gly 115 120 125Ala Thr Arg Ala Lys Ile Ile Gly Gly Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Thr Val Ile Lys Thr145 150 155 160Arg Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Ile 165 170 175Ser Ala Val Ile Trp Leu Leu Tyr Gly Leu Ala Leu Lys Asp Ile Tyr 180 185 190Val Ala Phe Pro Asn Val Leu Gly Phe Ala Leu Gly Ala Leu Gln Met 195 200 205Ile Leu Tyr Val Val Tyr Lys Tyr Cys Lys Thr Ser Pro His Leu Gly 210 215 220Glu Lys Glu Val Glu Ala Ala Lys Leu Pro Glu Val Ser Leu Asp Met225 230 235 240Leu Lys Leu Gly Thr Val Ser Ser Pro Glu Pro Ile Ser Val Val Arg 245 250 255Gln Ala Asn Lys Cys Thr Cys Gly Asn Asp Arg Arg Ala Glu Ile Glu 260 265 270Asp Gly Gln Thr Pro Lys His Gly Lys Gln Ser Ser Ser Ala Ala Ala 275 280 285Thr18285PRTArabidopsis thaliana 18Met Ala Leu Phe Asp Thr His Asn Thr Trp Ala Phe Val Phe Gly Leu1 5 10 15Leu Gly Asn Leu Ile Ser Phe Ala Val Phe Leu Ser Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Cys Lys Lys Lys Thr Thr Glu Gly Phe Gln Ser Ile 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr Ala 50 55 60Thr Gln Lys Lys Asp Val Phe Leu Leu Val Thr Ile Asn Ser Phe Gly65 70 75 80Cys Phe Ile Glu Thr Ile Tyr Ile Ser Ile Phe Val Ala Phe Ala Ser 85 90 95Lys Lys Ala Arg Met Leu Thr Val Lys Leu Leu Leu Leu Met Asn Phe 100 105 110Gly Gly Phe Cys Leu Ile Leu Leu Leu Cys Gln Phe Leu Ala Lys Gly 115 120 125Thr Thr Arg Ala Lys Ile Ile Gly Gly Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Thr Val Ile Lys Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Ile 165 170 175Ser Ala Val Ile Trp Leu Leu Tyr Gly Leu Ala Leu Lys Asp Ile Tyr 180 185 190Val Ala Phe Pro Asn Val Ile Gly Phe Val Leu Gly Ala Leu Gln Met 195 200 205Ile Leu Tyr Val Val Tyr Lys Tyr Cys Lys Thr Pro Ser Asp Leu Val 210 215 220Glu Lys Glu Leu Glu Ala Ala Lys Leu Pro Glu Val Ser Ile Asp Met225 230 235 240Val Lys Leu Gly Thr Leu Thr Ser Pro Glu Pro Val Ala Ile Thr Val 245 250 255Val Arg Ser Val Asn Thr Cys Asn Cys Asn Asp Arg Asn Ala Glu Ile 260 265 270Glu Asn Gly Gln Gly Val Arg Asn Ser Ala Ala Thr Thr 275 280 28519294PRTArabidopsis thaliana 19Met Ala Leu Thr Asn Asn Leu Trp Ala Phe Val Phe Gly Ile Leu Gly1 5 10 15Asn Ile Ile Ser Phe Val Val Phe Leu Ala Pro Val Pro Thr Phe Val 20 25 30Arg Ile Cys Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Val Ser Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Met Gln 50 55 60Lys Asp Gly Thr Ala Phe Leu Leu Ile Thr Ile Asn Ala Phe Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Ile Val Leu Phe Val Ser Tyr Ala Asn Lys 85 90 95Lys Thr Arg Ile Ser Thr Leu Lys Val Leu Gly Leu Leu Asn Phe Leu 100 105 110Gly Phe Ala Ala Ile Val Leu Val Cys Glu Leu Leu Thr Lys Gly Ser 115 120 125Thr Arg Glu Lys Val Leu Gly Gly Ile Cys Val Gly Phe Ser Val Ser 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Val Val Val Arg Thr Arg145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Ile Ser 165 170 175Ala Val Thr Trp Leu Phe Tyr Gly Leu Ala Ile Lys Asp Phe Tyr Val 180 185 190Ala Leu Pro Asn Val Leu Gly Ala Phe Leu Gly Ala Val Gln Met Ile 195 200 205Leu Tyr Ile Ile Phe Lys Tyr Tyr Lys Thr Pro Val Ala Gln Lys Thr 210 215 220Asp Lys Ser Lys Asp Val Ser Asp His Ser Ile Asp Ile Ala Lys Leu225

230 235 240Thr Thr Val Ile Pro Gly Ala Val Leu Asp Ser Ala Val His Gln Pro 245 250 255Pro Ala Leu His Asn Val Pro Glu Thr Lys Ile Gln Leu Thr Glu Val 260 265 270Lys Ser Gln Asn Met Thr Asp Pro Lys Asp Gln Ile Asn Lys Asp Val 275 280 285Gln Lys Gln Ser Gln Val 29020281PRTArabidopsis thaliana 20Met Val Leu Thr His Asn Val Leu Ala Val Thr Phe Gly Val Leu Gly1 5 10 15Asn Ile Ile Ser Phe Ile Val Phe Leu Ala Pro Val Pro Thr Phe Val 20 25 30Arg Ile Cys Lys Lys Lys Ser Ile Glu Gly Phe Glu Ser Leu Pro Tyr 35 40 45Val Ser Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Leu Gln 50 55 60Lys Asp Gly Ala Gly Phe Leu Leu Ile Thr Ile Asn Ala Val Gly Cys65 70 75 80Phe Ile Glu Thr Ile Tyr Ile Ile Leu Phe Ile Thr Tyr Ala Asn Lys 85 90 95Lys Ala Arg Ile Ser Thr Leu Lys Val Leu Gly Leu Leu Asn Phe Leu 100 105 110Gly Phe Ala Ala Ile Ile Leu Val Cys Glu Leu Leu Thr Lys Gly Ser 115 120 125Asn Arg Glu Lys Val Leu Gly Gly Ile Cys Val Gly Phe Ser Val Cys 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Val Val Ile Arg Thr Lys145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Ile Ser 165 170 175Ala Ile Thr Trp Leu Phe Tyr Gly Leu Ala Ile Lys Asp Phe Tyr Val 180 185 190Ala Leu Pro Asn Ile Leu Gly Ala Phe Leu Gly Ala Val Gln Met Ile 195 200 205Leu Tyr Val Ile Phe Lys Tyr Tyr Lys Thr Pro Leu Val Val Asp Glu 210 215 220Thr Glu Lys Pro Lys Thr Val Ser Asp His Ser Ile Asn Met Val Lys225 230 235 240Leu Ser Ser Thr Pro Ala Ser Gly Asp Leu Thr Val Gln Pro Gln Thr 245 250 255Asn Pro Asp Val Ser His Pro Ile Lys Thr His Gly Gly Asp Leu Glu 260 265 270Asp Gln Met Asp Lys Lys Met Pro Asn 275 28021292PRTArabidopsis thaliana 21Met Gly Val Met Ile Asn His His Phe Leu Ala Phe Ile Phe Gly Ile1 5 10 15Leu Gly Asn Val Ile Ser Phe Leu Val Phe Leu Ala Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Arg Lys Ser Thr Glu Ser Phe Gln Ser Leu 35 40 45Pro Tyr Gln Val Ser Leu Phe Ser Cys Met Leu Trp Leu Tyr Tyr Ala 50 55 60Leu Ile Lys Lys Asp Ala Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly65 70 75 80Cys Val Val Glu Thr Leu Tyr Ile Ala Met Phe Phe Ala Tyr Ala Thr 85 90 95Arg Glu Lys Arg Ile Ser Ala Met Lys Leu Phe Ile Ala Met Asn Val 100 105 110Ala Phe Phe Ser Leu Ile Leu Met Val Thr His Phe Val Val Lys Thr 115 120 125Pro Pro Leu Gln Val Ser Val Leu Gly Trp Ile Cys Val Ala Ile Ser 130 135 140Val Ser Val Phe Ala Ala Pro Leu Met Ile Val Ala Arg Val Ile Lys145 150 155 160Thr Lys Ser Val Glu Tyr Met Pro Phe Thr Leu Ser Phe Phe Leu Thr 165 170 175Ile Ser Ala Val Met Trp Phe Ala Tyr Gly Leu Phe Leu Asn Asp Ile 180 185 190Cys Ile Ala Ile Pro Asn Val Val Gly Phe Val Leu Gly Leu Leu Gln 195 200 205Met Val Leu Tyr Leu Val Tyr Arg Asn Ser Asn Glu Lys Pro Glu Lys 210 215 220Ile Asn Ser Ser Glu Gln Gln Leu Lys Ser Ile Val Val Met Ser Pro225 230 235 240Leu Gly Val Ser Glu Val His Pro Val Val Thr Glu Ser Val Asp Pro 245 250 255Leu Ser Glu Ala Val His His Glu Asp Leu Ser Lys Val Thr Lys Val 260 265 270Glu Glu Pro Ser Ile Glu Asn Gly Lys Cys Tyr Val Glu Ala Thr Arg 275 280 285Pro Glu Thr Val 29022307PRTOryza sativa 22Met Ala Gly Gly Phe Leu Ser Met Ala Asn Pro Ala Val Thr Leu Ser1 5 10 15Gly Val Ala Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Val 20 25 30Ala Thr Phe Leu Gln Val Tyr Lys Lys Lys Ser Thr Gly Gly Tyr Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Ser Val Leu Trp Ile Phe 50 55 60Tyr Ala Leu Val Lys Thr Asn Ser Arg Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Gly Val Glu Ala Ala Tyr Ile Val Leu Tyr Leu Val Tyr 85 90 95Ala Pro Arg Arg Ala Arg Leu Arg Thr Leu Ala Phe Phe Leu Leu Leu 100 105 110Asp Val Ala Ala Phe Ala Leu Ile Val Val Thr Thr Leu Tyr Leu Val 115 120 125Pro Lys Pro His Gln Val Lys Phe Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Ile Ile Phe Lys Val Ile145 150 155 160Lys Thr Lys Ser Val Glu Phe Met Pro Ile Gly Leu Ser Val Cys Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Cys Tyr Gly Leu Phe Thr Lys Asp 180 185 190Pro Tyr Val Met Tyr Pro Asn Val Gly Gly Phe Phe Phe Ser Cys Val 195 200 205Gln Met Gly Leu Tyr Phe Trp Tyr Arg Lys Pro Arg Asn Thr Ala Val 210 215 220Leu Pro Thr Thr Ser Asp Ser Met Ser Pro Ile Ser Ala Ala Ala Ala225 230 235 240Ala Thr Gln Arg Val Ile Glu Leu Pro Ala Gly Thr His Ala Phe Thr 245 250 255Ile Leu Ser Val Ser Pro Ile Pro Ile Leu Gly Val His Lys Val Glu 260 265 270Val Val Ala Ala Glu Gln Ala Ala Asp Gly Val Ala Ala Ala Ala Ala 275 280 285Ala Asp Lys Glu Leu Leu Gln Asn Lys Pro Glu Val Ile Glu Ile Thr 290 295 300Ala Ala Val30523300PRTOryza sativa 23Met Val Gln Ala Leu Val Phe Ala Val Gly Ile Val Gly Asn Ile Leu1 5 10 15Ser Phe Leu Val Ile Leu Ala Pro Val Pro Thr Phe Tyr Arg Val Tyr 20 25 30Lys Lys Lys Ser Thr Glu Ser Phe Gln Ser Val Pro Tyr Ala Val Ala 35 40 45Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Leu Leu Thr Ser Asp 50 55 60Leu Leu Leu Leu Ser Ile Asn Ser Ile Gly Cys Leu Val Glu Ser Leu65 70 75 80Tyr Leu Thr Val Tyr Leu Leu Tyr Ala Pro Arg Gln Ala Met Ala Phe 85 90 95Thr Leu Lys Leu Val Cys Ala Met Asn Leu Ala Leu Phe Ala Ala Val 100 105 110Val Ala Ala Leu Gln Leu Leu Val Lys Ala Thr Asp Arg Arg Val Thr 115 120 125Leu Ala Gly Gly Ile Gly Ala Ser Phe Ala Leu Ala Val Phe Val Ala 130 135 140Pro Leu Thr Ile Ile Arg Gln Val Ile Arg Thr Lys Ser Val Glu Phe145 150 155 160Met Pro Phe Trp Leu Ser Phe Phe Leu Thr Leu Ser Ala Val Val Trp 165 170 175Phe Phe Tyr Gly Leu Leu Met Lys Asp Phe Phe Val Ala Thr Pro Asn 180 185 190Val Leu Gly Leu Leu Phe Gly Leu Ala Gln Met Val Leu Tyr Val Val 195 200 205Tyr Lys Asn Pro Lys Lys Asn Ser Ala Val Ser Glu Ala Ala Ala Ala 210 215 220Gln Gln Val Glu Val Lys Asp Gln Gln Gln Leu Gln Met Gln Leu Gln225 230 235 240Ala Ser Pro Ala Val Ala Pro Leu Asp Val Asp Ala Asp Ala Asp Ala 245 250 255Asp Leu Glu Ala Ala Ala Pro Ala Thr Pro Gln Arg Pro Ala Asp Asp 260 265 270Asp Ala Ile Asp His Arg Ser Val Val Val Asp Ile Pro Pro Pro Pro 275 280 285Gln Pro Pro Pro Ala Leu Pro Ala Val Glu Val Ala 290 295 30024296PRTOryza sativa 24Met Ala Gly Leu Ser Leu Gln His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Leu Ile Ser Phe Thr Thr Tyr Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Ile Lys Ser Asn Glu Ala Leu Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Val Met Tyr Leu Ala Tyr Ala Pro 85 90 95Lys Lys Ala Lys Val Phe Thr Thr Lys Ile Leu Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Val Ile Leu Leu Leu Thr Leu Leu Leu Ser His Gly 115 120 125Glu Gln Arg Val Val Ser Leu Gly Trp Val Cys Val Ala Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Ile Lys Arg Val Ile Gln Ser145 150 155 160Arg Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Val Val Gln Met 195 200 205Gly Leu Tyr Val Phe Tyr Met Asn Ala Thr Pro Val Ala Gly Glu Gly 210 215 220Lys Glu Gly Lys Gly Lys Leu Ala Ala Ala Glu Glu Leu Pro Val Val225 230 235 240Val Asn Val Gly Lys Leu Ala Ala Ala Thr Pro Asp Arg Ser Thr Gly 245 250 255Ala Val His Val His Pro Val Pro Arg Ser Cys Ala Ala Glu Ala Ala 260 265 270Ala Ala Glu Pro Glu Val Leu Val Asp Ile Pro Pro Pro Pro Pro Pro 275 280 285Arg Ala Val Glu Val Ala Ala Val 290 29525303PRTOryza sativa 25Met Ala Gly Met Ser Leu Gln His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Ser Lys Ser Thr Gln Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Leu Lys Ser Asp Glu Cys Leu Leu Ile Thr Ile Asn Ser Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Ala Val Tyr Leu Val Tyr Ala Pro 85 90 95Lys Lys Ala Lys Met Phe Thr Ala Lys Leu Leu Leu Leu Val Asn Val 100 105 110Gly Val Phe Gly Leu Ile Leu Leu Leu Thr Leu Leu Leu Ser Ala Gly 115 120 125Asp Arg Arg Ile Val Val Leu Gly Trp Val Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Ile Arg Leu Val Val Arg Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Ser Leu Thr Ile 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Val Leu Gly Phe Ser Phe Gly Val Ile Gln Met 195 200 205Gly Leu Tyr Ala Met Tyr Arg Asn Ser Thr Pro Lys Ala Val Leu Thr 210 215 220Lys Glu Val Glu Ala Ala Thr Ala Thr Gly Asp Asp Asp His Ser Ala225 230 235 240Ala Gly Val Lys Glu His Val Val Asn Ile Ala Lys Leu Ser Ala Ala 245 250 255Val Asp Val Val Lys Thr Arg Glu Val His Pro Val Asp Val Glu Ser 260 265 270Pro Pro Ala Glu Ala Pro Pro Glu Glu Asp Asp Lys Ala Ala Ala Ala 275 280 285Thr Ala Ala Ala Val Ala Gly Ala Gly Glu Lys Lys Val Ala Ala 290 295 30026319PRTOryza sativa 26Met Ala Phe Met Ser Met Glu Arg Ser Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Leu Ile Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Thr Pro Tyr Val Val Thr Leu Phe Ser Cys Met Leu Trp Met Tyr Tyr 50 55 60Ala Phe Val Lys Ser Gly Ala Glu Leu Leu Val Thr Ile Asn Gly Val65 70 75 80Gly Cys Val Ile Glu Thr Val Tyr Leu Ala Met Tyr Leu Ala Tyr Ala 85 90 95Pro Lys Ser Ala Arg Met Leu Thr Ala Lys Met Leu Leu Gly Leu Asn 100 105 110Ile Gly Leu Phe Gly Val Ile Ala Leu Val Thr Leu Leu Leu Ser Arg 115 120 125Gly Glu Leu Arg Val His Val Leu Gly Trp Ile Cys Val Ala Val Ser 130 135 140Leu Ser Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Leu Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Ile Trp Phe Leu Tyr Gly Leu Leu Lys Lys Asp Val 180 185 190Phe Val Ala Leu Pro Asn Val Leu Gly Phe Val Phe Gly Val Ala Gln 195 200 205Met Ala Leu Tyr Met Ala Tyr Arg Ser Lys Lys Pro Leu Val Ala Ser 210 215 220Ser Ser Ser Ala Val Val Ala Ala Gly Leu Glu Ile Lys Leu Pro Glu225 230 235 240His Val Lys Glu Val Gln Ala Val Ala Lys Gly Ala Val Ala Ala Ala 245 250 255Pro Glu Gly Arg Ile Ser Cys Gly Ala Glu Val His Pro Ile Asp Asp 260 265 270Val Met Pro Ser Glu Val Val Glu Val Lys Val Asp Asp Glu Glu Thr 275 280 285Asn Arg Thr Asp Glu Met Ala Gly Asp Gly Asp His Ala Met Val Arg 290 295 300Thr Glu Gln Ile Ile Lys Pro Asp Met Ala Ile Val Val Glu Val305 310 31527252PRTMedicago truncatula 27Met Phe Pro Phe Ser Asn Leu Lys Met Val Leu Leu Phe Gly Phe Leu1 5 10 15Gly Ile Val Thr Phe Met Ser Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Ser Ile Tyr Lys Lys Lys Ser Ser Glu Gly Phe His Ser Ile Pro Tyr 35 40 45Val Val Thr Leu Leu Ser Thr Leu Leu Phe Val Tyr Tyr Gly Phe Leu 50 55 60Lys Thr Asn Ala Ile Phe Leu Ile Thr Ile Asn Ser Ile Gly Cys Val65 70 75 80Met Glu Val Ala Tyr Leu Ile Met Tyr Ile Thr Tyr Ala Pro Lys Lys 85 90 95Leu Lys Ile Ser Thr Leu Val Leu Ile Leu Ile Val Asp Met Gly Gly 100 105 110Phe Gly Leu Thr Met Ile Ile Thr Thr Phe Ile Val Lys Gly Ser Phe 115 120 125His Val Gln Val Val Gly Met Ile Cys Thr Ile Phe Asn Ile Gly Met 130 135 140Phe Ala Ala Pro Leu Ser Ile Met Lys Lys Val Ile Lys Thr Arg Ser145 150 155 160Val Glu Tyr Met Pro Phe Pro Leu Ser Leu Phe Leu Thr Ile Cys Ala 165 170 175Thr Met Trp Phe Phe Tyr Gly Phe Phe Asp Lys Asp Lys Tyr Ile Met 180 185 190Leu Pro Asn Gly Leu Gly Phe Leu Leu Gly Val Ser Gln Met Ile Leu 195 200 205Tyr Leu Ile Tyr Lys Asn Ala Lys Asn Asn Val Glu Ala Ser Ser Thr 210 215 220Asn Gln Leu Gln Glu His Gly Cys Asp Gly Gly Asn Asn Gln Ile Phe225 230 235 240Pro Thr Val Val Glu Met Lys Glu Ile Asn Ile Val 245 25028270PRTMedicago truncatula 28Met Ala Leu Phe Tyr Ser Glu Tyr Trp Ala Phe Val Phe Gly Val Ile1 5 10 15Gly Asn Val Ile Ser Cys Met Thr Phe Leu Ala Pro Leu Pro Thr Phe 20 25 30Tyr Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Val Pro

35 40 45Tyr Val Thr Ala Leu Leu Ser Ala Met Leu Trp Ile Tyr Tyr Ala His 50 55 60Val Lys Asn Lys Ala Thr Leu Leu Leu Leu Thr Ile Asn Ile Tyr Gly65 70 75 80Phe Gly Ile Glu Ala Ile Tyr Ile Ile Ile Phe Leu Leu Tyr Ala Ser 85 90 95Asn Lys Ala Arg Leu Ser Thr Ile Lys Leu Leu Phe Leu Thr Val Cys 100 105 110Gly Tyr Gly Thr Met Val Ile Leu Thr Thr Tyr Leu Thr Lys Gly Ser 115 120 125Lys Arg Leu Ser Ile Ile Gly Trp Ile Cys Met Val Phe Asn Ile Cys 130 135 140Val Phe Ala Ser Pro Leu Phe Ile Leu Lys Gln Val Ile Lys Thr Lys145 150 155 160Ser Val Ala Phe Met Pro Leu Asn Leu Ser Phe Phe Leu Thr Leu Asn 165 170 175Ala Ile Val Trp Phe Phe Tyr Gly Leu Leu Ile Asp Asp Phe Tyr Ile 180 185 190Ala Ile Pro Asn Thr Leu Gly Phe Val Phe Gly Ile Val Gln Met Val 195 200 205Ile Tyr Leu Ile Tyr Lys Asp Ala Ile Pro Leu Glu Ser Thr Lys Leu 210 215 220Gln Lys Pro Asn Asp His Val Leu Asn Ile Cys Glu Asp Val Pro Asn225 230 235 240Gly Ala Leu Gln Pro Asp Pro Asn Gln Val Val Lys Ser Gly Ala Pro 245 250 255Ala Val Ala Val Ile Gly Asp Glu Asp Pro Asn Asn Gly Lys 260 265 27029255PRTMedicago truncatula 29Met Ala Met Thr Arg Glu Ser Trp Ala Phe Val Phe Gly Ile Ile Gly1 5 10 15Asn Ile Ile Ser Phe Ala Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Val Ile Phe Lys Lys Lys Ser Ala Glu Gly Phe Gln Ala Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Val 50 55 60Lys Arg Glu Ser Ala Leu Leu Leu Ile Thr Ile Asn Thr Phe Gly Ile65 70 75 80Val Val Glu Ser Ala Tyr Ile Ile Met Phe Leu Ile Tyr Ala Pro Lys 85 90 95Lys Gln Arg Leu Ser Thr Ile Lys Leu Leu Leu Leu Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ser Thr Leu Tyr Leu Ser Lys Gly Ala 115 120 125Lys Arg Leu Ala Ile Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Ser 130 135 140Val Phe Ala Thr Pro Leu Phe Val Ile Ser Lys Val Ile Arg Ser Arg145 150 155 160Ser Val Glu Tyr Met Pro Phe Phe Leu Ser Phe Phe Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Arg Asp Tyr Tyr Val 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Val Phe Gly Ile Ile Gln Met Val 195 200 205Val Tyr Leu Ile Tyr Arg Asn Ala Thr Pro Val Val Glu Ala Pro Met 210 215 220Lys Gly Gln Glu Leu Ser Gly Gly His Ile Ile Asp Val Val Lys Ile225 230 235 240Gly Thr Asp Pro Asn Arg Ala Gly Gly Gly Ala Gly Ser Lys Val 245 250 25530255PRTMedicago truncatula 30Met Ala Met Thr Arg Glu Ser Trp Ala Phe Val Phe Gly Ile Ile Gly1 5 10 15Asn Ile Ile Ser Phe Ala Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Val Ile Phe Lys Lys Lys Ser Ala Glu Gly Phe Gln Ala Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Val 50 55 60Lys Arg Glu Ser Ala Leu Leu Leu Ile Thr Ile Asn Thr Phe Gly Ile65 70 75 80Val Val Glu Ser Ala Tyr Ile Ile Met Phe Leu Ile Tyr Ala Pro Lys 85 90 95Lys Gln Arg Leu Ser Thr Ile Lys Leu Leu Leu Leu Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ser Thr Leu Tyr Leu Ser Lys Gly Ala 115 120 125Lys Arg Leu Ala Ile Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Ser 130 135 140Val Phe Ala Ala Pro Leu Phe Val Ile Ser Lys Val Ile Arg Ser Arg145 150 155 160Ser Val Glu Tyr Met Pro Phe Phe Leu Ser Phe Phe Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Arg Asp Tyr Tyr Val 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Val Phe Gly Ile Ile Gln Met Val 195 200 205Val Tyr Leu Ile Tyr Arg Asn Ala Thr Pro Val Val Glu Ala Pro Met 210 215 220Lys Gly Gln Glu Leu Ser Gly Gly His Ile Ile Asp Val Val Lys Ile225 230 235 240Gly Thr Asp Ser Asn Arg Ala Gly Gly Gly Ala Gly Ser Lys Val 245 250 25531268PRTMedicago truncatulamisc_feature(192)..(192)Xaa can be any naturally occurring amino acidmisc_feature(199)..(199)Xaa can be any naturally occurring amino acid 31Met Ala Ile Ser His Asn Thr Leu Ala Phe Thr Phe Gly Met Leu Gly1 5 10 15Asn Val Ile Ser Phe Leu Val Phe Leu Ala Pro Ile Ser Thr Phe Tyr 20 25 30Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Lys Lys Asp Ala Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Val Glu Thr Ile Tyr Ile Ile Leu Tyr Ile Ile Tyr Ala Pro Arg Asp 85 90 95Ala Arg Asn Leu Thr Phe Lys Leu Leu Ser Ala Met Asn Val Gly Ser 100 105 110Phe Ala Leu Ile Leu Ile Val Thr Asn Tyr Ala Val His Gly Pro Leu 115 120 125Arg Val Gln Val Leu Gly Trp Val Cys Val Ser Leu Ser Val Ser Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Val Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Asn Leu Ser Phe Thr Leu Thr Leu Ser Ala 165 170 175Thr Met Trp Phe Gly Tyr Gly Phe Phe Leu Lys Asp Ile Cys Ile Xaa 180 185 190Leu Pro Asn Val Leu Gly Xaa Val Leu Gly Leu Leu Gln Met Leu Leu 195 200 205Tyr Ala Ile Tyr Arg Asn Gly Gly Glu Lys Ala Met Lys Lys Glu Lys 210 215 220Lys Ala Pro Ile Glu Pro Pro Lys Ser Ile Val Ile Glu Thr Gln Leu225 230 235 240Glu Lys Ile Glu Gln Glu Lys Lys Asn Lys Asp Asp Asp Asn Glu Glu 245 250 255Lys Asp Lys Ser Glu Glu Pro Ile Gly Cys Gly Val 260 26532268PRTMedicago truncatula 32Met Ala Ile Ser His Asn Thr Leu Ala Phe Ala Phe Gly Met Leu Gly1 5 10 15Asn Val Ile Ser Phe Met Val Phe Leu Ala Pro Met Thr Thr Phe Tyr 20 25 30Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala Phe Leu 50 55 60Lys Lys Asp Glu Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Val Glu Leu Ile Tyr Ile Ile Leu Tyr Ile Ile Tyr Ala Thr Lys Asp 85 90 95Ala Arg Lys Leu Thr Ile Lys Leu Leu Leu Ala Met Asn Ile Gly Ser 100 105 110Phe Gly Leu Ile Leu Leu Val Thr Lys Tyr Ala Val His Gly Pro Ile 115 120 125Arg Val Gln Val Leu Gly Trp Ile Cys Val Ser Ile Ser Val Ser Val 130 135 140Phe Ala Ala Pro Leu Thr Ile Val Ala Gln Val Val Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Asn Leu Ser Phe Thr Leu Thr Leu Ser Ala 165 170 175Ile Met Trp Phe Gly Tyr Gly Leu Phe Leu Lys Asp Ile Cys Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Ala Leu Gly Leu Val Gln Met Ile Leu 195 200 205Tyr Cys Ile Tyr Arg Asn Gly Asp Lys Lys Lys Ala Asn Ser Lys Ala 210 215 220Ala Leu Lys Ser Val Val Ile Glu Ser Ser Leu Gly Gly Thr Gly Glu225 230 235 240Val Phe Gln Val Glu Lys Asn Asp Gly Glu Glu Glu Glu Glu Lys Lys 245 250 255Lys Thr Ile Glu Glu Thr Glu Tyr Asp Ser Lys Val 260 26533269PRTMedicago truncatula 33Met Asp Pro His Asp His Asp Arg Leu Ala Phe Ile Phe Gly Ile Leu1 5 10 15Gly Asn Ile Ile Ser Ser Met Val Tyr Leu Ala Pro Leu Pro Thr Phe 20 25 30Tyr Arg Ile Trp Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro 35 40 45Tyr Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Gly Phe 50 55 60Val Lys Lys His Ala Phe Leu Leu Ile Thr Ile Asn Ser Ala Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Ile Val Thr Tyr Leu Ile Tyr Ala Thr Lys 85 90 95Asp Ala Arg Ile Leu Thr Ile Lys Leu Phe Met Ala Met Asn Val Ala 100 105 110Cys Ser Val Leu Ile Val Leu Thr Thr Gln Leu Ala Met His Gly Lys 115 120 125Leu Arg Val His Val Leu Gly Trp Ile Cys Thr Ser Phe Ala Ile Cys 130 135 140Val Phe Ala Ala Pro Leu Thr Ile Met Ala Lys Val Ile Arg Thr Lys145 150 155 160Ser Val Glu Phe Met Pro Ile Asn Leu Ser Phe Phe Leu Thr Leu Ser 165 170 175Ala Ile Val Trp Phe Phe Tyr Gly Leu Leu Leu His Asp Ile Cys Ile 180 185 190Ala Ile Pro Asn Val Leu Gly Phe Ile Leu Gly Leu Leu Gln Met Leu 195 200 205Leu Tyr Ala Ile Tyr Asn Lys Ser Val Lys Glu Glu Tyr Ala Leu Glu 210 215 220Pro Met Thr Asn Ile Val Ile Val Asn Pro Leu Gly Ile Pro Cys Glu225 230 235 240Val Phe Ser Leu Pro Val Ile Asp Asn Val Asn Lys Ile Glu Lys Glu 245 250 255Gly Ala Glu Glu Met Glu Lys Ser Val Glu Asn Leu Thr 260 26534288PRTMedicago truncatula 34Met Ala Met Ile Ser Met Asn His His Phe Leu Val Ile Ala Phe Gly1 5 10 15Leu Leu Gly Asn Ile Ile Ser Cys Met Val Tyr Leu Ala Pro Leu Pro 20 25 30Thr Phe Ile Gln Ile Tyr Lys Lys Lys Ser Thr Glu Cys Phe Gln Ser 35 40 45Leu Pro Tyr Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr 50 55 60Gly Ile Gln Thr Asn Ala Ile Phe Ile Val Ser Ile Asn Ala Phe Gly65 70 75 80Cys Val Ile Glu Ile Ile Tyr Cys Ile Met Tyr Ile Ala Tyr Ala Thr 85 90 95Lys Asp Ala Arg Lys Leu Thr Ile Lys Leu Cys Ala Ala Leu Asn Val 100 105 110Val Ser Phe Val Leu Ile Phe Leu Ile Ile Gln Phe Ser Ile Pro Glu 115 120 125Asn His Arg Val Gln Val Leu Gly Trp Ile Cys Thr Ser Ile Ser Ile 130 135 140Ser Val Phe Ala Ala Pro Leu Ser Ile Val Val Arg Val Val Lys Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Asn Leu Ser Leu Phe Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Phe Val Lys Arg Asp Ile Cys 180 185 190Ile Tyr Leu Pro Asn Val Val Gly Phe Ile Leu Gly Ile Ile Gln Met 195 200 205Val Leu Tyr Gly Tyr Tyr Ser Lys Tyr Ser Val Glu Lys Glu Lys Glu 210 215 220Gln Ala Val Ile Asn Ile Val Val Val Asn Pro Leu Gly Ser Ser Glu225 230 235 240Val Phe Pro Ile Pro Leu Asp Glu Asn Lys Glu Ser Ile Glu Asp Val 245 250 255Ile Asn Gln Gln Phe Gln Val Lys Lys Val Gly Glu Glu Asp Ala Lys 260 265 270Glu Lys His Asp Asn Asn Val Glu Ala Ile Glu Phe Gln Cys Val Val 275 280 28535265PRTPetunia hybrida 35Met Ala Gln Leu Arg Ala Asp Asp Leu Ser Phe Ile Phe Gly Leu Leu1 5 10 15Gly Asn Ile Val Ser Phe Met Val Phe Leu Ala Pro Val Pro Thr Phe 20 25 30Tyr Lys Ile Tyr Lys Arg Lys Ser Ser Glu Gly Tyr Gln Ala Ile Pro 35 40 45Tyr Met Val Ala Leu Phe Ser Ala Gly Leu Leu Leu Tyr Tyr Ala Tyr 50 55 60Leu Arg Lys Asn Ala Tyr Leu Ile Val Ser Ile Asn Gly Phe Gly Cys65 70 75 80Ala Ile Glu Leu Thr Tyr Ile Ser Leu Phe Leu Phe Tyr Ala Pro Arg 85 90 95Lys Ser Lys Ile Phe Thr Gly Trp Leu Met Leu Leu Glu Leu Gly Ala 100 105 110Leu Gly Met Val Met Pro Ile Thr Tyr Leu Leu Ala Glu Gly Ser His 115 120 125Arg Val Met Ile Val Gly Trp Ile Cys Ala Ala Ile Asn Val Ala Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Met Arg Gln Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Thr Leu Ser Leu Phe Leu Thr Leu Cys Ala 165 170 175Thr Met Trp Phe Phe Tyr Gly Phe Phe Lys Lys Asp Phe Tyr Ile Ala 180 185 190Phe Pro Asn Ile Leu Gly Phe Leu Phe Gly Ile Val Gln Met Leu Leu 195 200 205Tyr Phe Val Tyr Lys Asp Ser Lys Arg Ile Asp Asp Glu Lys Ser Asp 210 215 220Pro Val Arg Glu Ala Thr Lys Ser Lys Glu Gly Val Glu Ile Ile Ile225 230 235 240Asn Ile Glu Asp Asp Asn Ser Asp Asn Ala Leu Gln Ser Met Glu Lys 245 250 255Asp Phe Ser Arg Leu Arg Thr Ser Lys 260 26536301PRTCapsicum annuum 36Met Thr Gly Ile Ser Gly His Trp Ala Phe Ala Phe Gly Val Leu Gly1 5 10 15Asn Ile Ile Ser Phe Ile Val Phe Leu Ser Pro Ile Pro Thr Phe Tyr 20 25 30Thr Ile Tyr Lys Lys Lys Thr Ala Glu Gly Tyr Gln Ser Ile Pro Tyr 35 40 45Val Ile Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Phe Leu 50 55 60Lys Thr Asn Val Thr Leu Leu Ile Thr Ile Asn Ser Phe Gly Ile Phe65 70 75 80Ile Glu Thr Ile Tyr Val Gly Leu Tyr Leu Phe Tyr Ala Pro Lys Lys 85 90 95Ala Arg Val His Thr Val Lys Met Leu Leu Leu Thr Val Val Gly Gly 100 105 110Phe Gly Ala Ile Val Leu Val Thr Gln Phe Leu Phe Lys Gly Val Val 115 120 125Arg Gly Gln Ile Val Gly Trp Ile Cys Leu Ile Phe Ala Leu Ser Val 130 135 140Phe Val Ala Pro Leu Gly Ile Val Arg Gln Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Leu Leu Leu Ser Val Phe Leu Thr Leu Ser Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Ile Asn Ile Ala 180 185 190Ala Pro Asn Val Leu Gly Phe Ile Phe Gly Val Leu Gln Ile Val Leu 195 200 205Tyr Ala Ile Tyr Ser Lys Lys Glu Lys Val Ile Leu Lys Glu Gln Lys 210 215 220Leu Pro Glu Ile Gln Lys Pro Ala Val Ile Val Ala Asp Asp Asn Thr225 230 235 240Asn Ala Asn Lys Lys Leu Pro Glu Leu Thr His Glu Gln Ile Ile Asp 245 250 255Ile Val Lys Leu Ala Gly Leu Leu Thr Cys Thr Glu Lys Ser His Val 260 265 270Ala Thr Cys Pro His Asp Val Asn Cys Gly Val Glu Ala Thr Asn Val 275 280 285Glu Asn Asn Ile Pro Lys Leu Gln Thr Val Glu Ala Thr 290 295 30037260PRTLotus japonicus 37Met Ala Leu Ser His Asn Thr Leu Ala Phe Thr Phe Gly Met Leu Gly1 5 10 15Asn Val Ile Ser Phe Met Val Phe Leu Ala Pro Ile Ala Thr Phe Tyr

20 25 30Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala Met Val 50 55 60Lys Lys Asp Ala Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Ile Glu Ile Ile Tyr Ile Ile Leu Tyr Met Ile Tyr Ala Pro Arg Asp 85 90 95Ala Arg Asn Leu Thr Leu Lys Leu Phe Thr Ala Met Asn Val Gly Ser 100 105 110Phe Ala Leu Ile Leu Leu Val Thr His Phe Ala Val His Gly Pro Leu 115 120 125Arg Val Gln Val Leu Gly Trp Ile Cys Val Ser Ile Ala Val Ser Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Val Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Asn Leu Ser Phe Thr Leu Thr Leu Ser Ala 165 170 175Thr Met Trp Phe Gly Tyr Gly Leu Phe Leu Lys Asp Ile Cys Ile Ala 180 185 190Leu Pro Asn Ile Leu Gly Phe Gly Leu Gly Leu Ile Gln Met Val Leu 195 200 205Tyr Ala Ile Tyr Arg Asn Gly Asn Glu Lys Gly Lys Lys Pro Ala Ala 210 215 220Ala Leu Lys Ser Val Val Ile Glu Ile Pro Thr Ser Asn Val Ile Gly225 230 235 240Glu Glu Val Gly Glu Glu Lys Glu Lys Thr Glu Glu Pro Pro Val Asn 245 250 255Ala Cys Ala Ala 26038247PRTLotus japonicus 38Met Ala Met Thr Arg Glu Ser Trp Ala Phe Val Phe Gly Leu Met Gly1 5 10 15Asn Val Ile Ser Phe Met Val Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Gln Ile Tyr Lys Lys Lys Thr Ala Glu Gly Phe Gln Ala Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Val 50 55 60Lys Arg Glu Ser Ala Leu Leu Leu Ile Thr Ile Asn Thr Phe Gly Ile65 70 75 80Val Val Glu Ser Ile Tyr Ile Ala Phe Phe Leu Phe Tyr Ala Pro Lys 85 90 95Lys Ser Arg Leu Ser Thr Ile Lys Leu Leu Leu Leu Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ala Thr Leu Tyr Leu Ser Lys Gly Ala 115 120 125Lys Arg Leu Gln Ile Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Ser 130 135 140Val Phe Ala Ala Pro Leu Phe Ile Ile Ser Lys Val Ile Arg Thr Arg145 150 155 160Ser Val Glu Tyr Met Pro Phe Phe Leu Ser Phe Ser Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Met Leu Leu Arg Asp Tyr Tyr Val 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Val Phe Gly Ile Ile Gln Met Val 195 200 205Val Tyr Leu Ile Tyr Arg Asn Ala Thr Pro Val Val Ile Glu Glu Lys 210 215 220Val Lys Gly Gln Glu Met Ser Gly Asp His Ile Ile Asp Val Ala Lys225 230 235 240Gly Gly Ala Val Ser Lys Val 24539333PRTZea mays 39Met Ala Phe Leu Asn Met Glu Gln Gln Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Ile Ile Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Thr Pro Tyr Val Val Thr Leu Phe Ser Cys Met Leu Trp Ile Phe Tyr 50 55 60Ala Leu Leu Lys Ser Gly Ala Glu Leu Leu Val Thr Ile Asn Gly Val65 70 75 80Gly Cys Val Ile Glu Ala Ala Tyr Leu Ala Ala Tyr Leu Val Tyr Ala 85 90 95Pro Lys Ala Ala Arg Ala Leu Thr Ala Lys Met Leu Leu Gly Leu Asn 100 105 110Val Gly Val Phe Gly Leu Ala Ala Leu Ala Thr Met Val Val Ser Ser 115 120 125Ala Gly Leu Arg Val Arg Val Leu Gly Trp Ile Cys Val Ser Val Ala 130 135 140Leu Ser Val Phe Ala Ala Pro Leu Ser Ile Met Arg Gln Val Val Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Ile Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Ile Trp Phe Ala Tyr Gly Ala Leu Lys Arg Asp Val 180 185 190Phe Val Ala Phe Pro Asn Val Leu Gly Phe Val Phe Gly Val Ala Gln 195 200 205Ile Ala Leu Tyr Met Ala Tyr Arg Asn Lys Glu Pro Ala Ala Val Thr 210 215 220Val Glu Glu Ala Lys Leu Pro Glu His Ala Lys Glu Val Val Val Ala225 230 235 240Ala Ala Ala Ala Glu Ala Arg Ala Ser Cys Gly Ala Glu Val His Pro 245 250 255Ile Asp Ile Asp Ile Glu Ala Thr Pro Thr Pro Val Glu Glu Val His 260 265 270Glu Pro Gln Val Val Val Val Val Asp Val Asp Val Glu Pro Val Thr 275 280 285Cys Ala Gly Ala Ala Glu Ala Ala Ala Gly Ala Gly Ala Asp Ala Ser 290 295 300Gly Val Ala Asp Gly Gly Val Pro Gly Pro Met Ala Pro Pro Glu Gln305 310 315 320Leu Ala Ile Lys Pro Asp Met Ala Ile Ser Val Glu Ala 325 33040305PRTZea mays 40Met Ile Thr Val Gly His Pro Val Ala Phe Ala Val Gly Ile Leu Gly1 5 10 15Asn Ile Leu Ser Phe Leu Val Ile Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Tyr Ala Lys Lys Ser Thr Glu Ser Phe Gln Ser Val Pro Tyr 35 40 45Val Val Ala Leu Leu Ser Ala Thr Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Ser Thr Asp Leu Leu Leu Leu Ser Ile Asn Thr Val Ala Cys Val Ala65 70 75 80Glu Ser Val Tyr Leu Ala Val Tyr Leu Ala Tyr Ala Pro Gly Pro Ala 85 90 95Lys Ala Phe Thr Leu Lys Leu Leu Cys Ala Ile Asn Met Gly Leu Phe 100 105 110Gly Ala Met Val Ala Phe Leu Gln Phe Tyr Val Val Asp Thr Gln Arg 115 120 125Arg Val Ser Ile Ala Gly Gly Val Gly Ala Ala Phe Ala Leu Ala Val 130 135 140Phe Val Ala Pro Leu Ala Ile Ile Arg Arg Val Met Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Trp Leu Ser Phe Phe Leu Thr Val Ser Ala 165 170 175Val Val Trp Phe Phe Tyr Gly Leu Leu Ile Lys Asp Phe Phe Val Ala 180 185 190Met Pro Asn Val Leu Gly Leu Leu Phe Gly Leu Ala Gln Met Val Leu 195 200 205Phe Phe Val Tyr Arg Asn Arg Asn Pro Lys Lys Asn Gly Ala Val Ser 210 215 220Glu Met Gln Gln Ala Ala Val Gln Ala Asp Ala Glu Lys Glu Arg Arg225 230 235 240Ser His Ala Asn Ala Asp Gly Glu Ala Asp Val Arg Thr Val Ile Val 245 250 255Asp Ile Met Pro Pro Pro Pro Ala Met Met Arg His Ala Asp Arg Glu 260 265 270Ala Arg Gly Gly Ala Gly Thr Gly Arg Arg Ala Ala Ala Arg Glu Gln 275 280 285Gly Gly Ala Arg Arg Arg Glu Asp Arg Glu Ala Leu Gly Gly Gly Gly 290 295 300Ile30541289PRTHordeum vulgare 41Met Ala Gly Leu Ser Met Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Thr Ser Leu Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Phe Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Val Lys Thr Gly Glu Gly Leu Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Val Tyr Ile Ile Met Tyr Leu Val Tyr Ala Pro 85 90 95Arg Lys Ala Lys Ile Phe Thr Ala Lys Ile Val Leu Leu Leu Asn Val 100 105 110Ala Gly Phe Gly Leu Ile Phe Leu Leu Thr Leu Phe Ala Phe His Gly 115 120 125Glu Thr Arg Val Val Ser Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Val Ala Pro Leu Ser Ile Ile Gly Arg Val Ile Lys Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Met Ile Gln Met 195 200 205Val Leu Tyr Met Phe Tyr Met Asn Ala Thr Pro Val Val Ala Ser Asp 210 215 220Ala Lys Glu Gly Lys Glu Ala Trp Lys Val Pro Ala Glu Asp His Val225 230 235 240Val Val Ile Asn Val Gly Lys Ala Asp Lys Ser Ser Cys Ala Glu Val 245 250 255Arg Pro Val Ala Asp Val Pro Arg Arg Cys Ala Ala Glu Ala Ala Ala 260 265 270Pro Gly Gln Gln Val Met Ala Val Asp Phe Ala Arg Ser Val Glu Val 275 280 285Val42292PRTHordeum vulgare 42Met Gly Gly Leu Ser Ala Gln His Pro Trp Ala Phe Thr Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Asn Lys Ser Thr Gln Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Leu Lys Ser Asp Glu Tyr Leu Leu Ile Thr Ile Asn Thr Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Val Leu Tyr Leu Ala Tyr Ala Pro 85 90 95Lys Gln Ala Arg Leu Phe Thr Ala Lys Ile Leu Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Leu Ile Leu Leu Leu Thr Leu Leu Leu Thr Ala Gly 115 120 125Glu Arg Arg Val Val Met Leu Gly Trp Val Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Val Ala Pro Leu Ser Val Ile Arg Leu Val Val Arg Thr145 150 155 160Arg Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Ala 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Ala Phe Gly Val Ile Gln Met 195 200 205Gly Leu Tyr Ala Leu Tyr Arg Asn Ala Thr Pro Ile Pro Ala Pro Lys 210 215 220Glu Met Asp Ala Pro Glu Ser Glu Asp Gly Ala Val Lys Ala Pro Glu225 230 235 240His Val Val Asn Ile Ala Lys Leu Gly Thr Ala Ala Ala Ala Ile Glu 245 250 255Leu Asn Thr Asn His Pro Val Glu Pro Pro Pro Pro Met Lys Glu Gly 260 265 270Thr Ala Lys Ala Cys Ala Thr Gly Glu Lys Leu Asp Lys Ala Thr His 275 280 285Val Glu Gln Val 29043314PRTHordeum vulgare 43Met Ala Ala Val Gly Ser Pro Leu Ile Phe Ala Val Gly Ile Leu Gly1 5 10 15Asn Ile Leu Ser Phe Leu Val Ile Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Tyr Lys Arg Lys Ser Thr Glu Ser Phe Gln Ser Val Pro Tyr 35 40 45Ala Met Ala Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Thr Lys Asp Leu Leu Leu Leu Thr Ile Asn Thr Val Gly Cys Val Val65 70 75 80Glu Thr Ala Tyr Leu Ala Ile Tyr Leu Ala Tyr Ala Pro Lys Gln Ala 85 90 95Lys Ala Phe Thr Ala Lys Leu Val Cys Ile Met Asn Val Ala Leu Tyr 100 105 110Gly Ala Met Val Cys Val Leu Gln Leu Leu Val Arg Asp Gly Glu Ser 115 120 125Arg Val Thr Ile Ala Gly Gly Ile Gly Ser Ala Phe Ala Leu Ala Val 130 135 140Phe Val Ala Pro Leu Ala Ile Ile Arg Gln Val Ile Arg Thr Lys Ser145 150 155 160Val Glu Phe Leu Pro Phe Trp Leu Ser Phe Phe Leu Thr Ile Ser Ala 165 170 175Val Val Trp Phe Phe Tyr Gly Leu Leu Met Lys Asp Phe Phe Val Ala 180 185 190Thr Pro Asn Val Leu Gly Leu Leu Phe Gly Leu Ala Gln Met Ala Leu 195 200 205His Leu Val Tyr Lys Asn Pro Lys Lys Lys Gly Asp Val Ser Glu Val 210 215 220Gln Leu Pro Asp Asp Asp Glu Lys Asn Gln Leu Pro Leu His His Gln225 230 235 240Gln Gln Gln Gln Ala Gly Thr Thr Gly His Val Val Ala Pro Pro Ile 245 250 255Ile Asp Asp Gly Glu Gln Val Val Asn Gly Ser Glu Asp Asp Val Gly 260 265 270Gly Asn Lys Gln Gln Ser Val Ser Val Met Asp Ile Val Leu Pro Pro 275 280 285Pro Glu Glu His Pro Thr Leu Pro Pro Leu Asp His Pro Ala Pro Leu 290 295 300Pro Pro Met Arg Met Ala Val Glu Val Val305 31044287PRTHordeum vulgare 44Met Ala Gly Gly Leu Phe Ser Met Ala His Pro Trp Ala Ser Ala Phe1 5 10 15Gly Ile Leu Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Thr 20 25 30Pro Thr Phe Leu Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Cys Thr Leu Trp Ile Leu 50 55 60Tyr Ala Leu Val Lys Thr Asn Ser Ser Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Val Val Glu Ala Phe Tyr Ile Val Leu Tyr Leu Val Tyr 85 90 95Ala Pro Arg Pro Ala Arg Met Arg Ala Leu Ala Phe Phe Leu Leu Leu 100 105 110Asn Val Ala Ala Phe Ser Leu Ile Val Ala Val Thr Val Phe Leu Val 115 120 125Pro Gln Pro Ser Arg Val Lys Val Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Val Ile Phe Val Val Ile145 150 155 160Lys Thr Lys Ser Ala Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Phe Tyr Gly Leu Phe Thr Lys Asp 180 185 190Ile Tyr Val Thr Leu Pro Asn Val Gly Gly Phe Phe Phe Gly Val Ala 195 200 205Gln Met Thr Leu Tyr Phe Cys Tyr Arg Lys Pro Asp Thr Ser Ala Leu 210 215 220Val Leu Pro Thr Gly Ile His Asp Val Ser Thr Glu Ala Ala Ala Gln225 230 235 240Gln Glu Val Glu Leu Pro Glu Gly Thr His Pro Ala Val Ala Met Leu 245 250 255Thr Val Ser Thr Leu Pro Met Leu Ala Glu Leu Gln Lys Met Glu Gln 260 265 270Glu Ile Ser Ser Pro Thr Pro Arg Lys Gly Tyr Ile Lys Ala Phe 275 280 28545288PRTVitis vinifera 45Met Ala Met Phe Thr Val Gly His His Pro Trp Val Phe Ala Ser Gly1 5 10 15Ile Leu Gly Asn Leu Met Ser Phe Leu Val Tyr Leu Ala Pro Ile Pro 20 25 30Thr Phe Thr Arg Val Ile Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Val Pro Tyr Val Ile Ala Leu Phe Ser Ala Met Leu Trp Met Tyr Tyr 50 55 60Gly Leu Val Asn Thr Asn Ala Ser Phe Leu Leu Ser Val Asn Gly Phe65 70 75 80Gly Cys Phe Ile Glu Ile Ile Tyr Ile Ser Ile Tyr Leu Ile Phe Ala 85 90 95Pro Arg Arg Ala Arg Ile Leu Thr Leu Arg Leu Leu Leu Leu Ile Asn 100 105 110Leu Gly Ala Phe Cys Leu Ile Leu Ile Val Thr Asn Phe Met Val Lys 115 120 125Arg Pro His Arg Val Lys Ala Val Gly Trp Val Cys Leu Ile Phe Ala 130 135 140Val Ser Val Phe Ala Ala Pro Leu Ser Ile Met Ala Ser Ile Leu Tyr145 150 155

160Arg Leu Val Ile Arg Thr Lys Ser Val Glu Phe Met Pro Leu Pro Leu 165 170 175Ser Ile Cys Leu Thr Leu Ser Ala Val Gly Trp Phe Phe Tyr Gly Ile 180 185 190Leu Gln Met Asp Leu Tyr Ile Ala Met Pro Asn Thr Leu Gly Phe Val 195 200 205Phe Gly Leu Ile Gln Met Ile Leu Tyr Ala Met Tyr Arg Asn Ser Thr 210 215 220Pro Val Thr Lys Glu Pro Lys Leu Pro Glu Gln Val Ile Asp Ile Val225 230 235 240Lys Leu Asn Thr Asn Ser Thr Pro Glu Val His Pro Val Ser Thr Leu 245 250 255Gln Pro Asn Cys Val Glu Asn Glu Gly Gly Asn Gly Gln Asn Ala Arg 260 265 270Lys Glu Thr Glu His Ala Glu Glu Ser Met Gly Gly Ser Asn Arg Val 275 280 28546341PRTVitis vinifera 46Met Ala Met Leu Thr Val Pro His Met Ala Phe Ala Phe Gly Ile Leu1 5 10 15Gly Asn Ile Val Ser Phe Leu Val Tyr Leu Ser Pro Leu Pro Thr Phe 20 25 30Tyr Arg Ile Tyr Lys Arg Lys Ser Thr Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Ser Val Ala Leu Phe Ser Ala Met Leu Leu Leu Tyr Tyr Ala Phe 50 55 60Leu Lys Thr Asp Asn Gln Ile Met Leu Ile Thr Ile Asn Ser Val Gly65 70 75 80Thr Cys Ile Glu Ala Thr Tyr Leu Leu Val Tyr Met Ile Tyr Ala Pro 85 90 95Arg Thr Ala Lys Ile Tyr Thr Ala Lys Leu Leu Leu Leu Phe Asn Thr 100 105 110Gly Val Tyr Gly Ala Ile Val Leu Ser Thr Phe Phe Leu Ser Lys Gly 115 120 125His Arg Arg Ala Lys Ile Val Gly Trp Val Cys Ala Ala Phe Ser Leu 130 135 140Cys Val Phe Ala Ala Pro Leu Ser Ile Met Arg Leu Val Ile Arg Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Pro Leu Ser Phe Phe Leu Thr Ile 165 170 175Cys Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Ile Arg Asp Phe Tyr 180 185 190Ile Ala Phe Pro Asn Ile Leu Gly Phe Ala Phe Gly Ile Ala Gln Met 195 200 205Ile Leu Tyr Thr Ile Tyr Lys Asn Ala Lys Lys Gly Val Leu Ala Glu 210 215 220Phe Lys Leu Gln Glu Leu Pro Asn Gly Leu Val Phe Pro Thr Leu Lys225 230 235 240Lys Ala Glu Asn Thr Asp Thr Asn Pro Asn Asp Gln Pro Glu Asp Thr 245 250 255Ala Met Thr Glu Gly Gly Ala Arg Asp Lys Ala Val Glu Pro Ser Gly 260 265 270Glu Leu Lys His Asn Ser Ser Ser Leu Val Val Arg Phe Cys Leu Arg 275 280 285Ala Leu Arg Leu Ser Phe His His Val Ser Phe Ser Arg Ile Ile Ala 290 295 300Tyr Thr Asn Gln Arg Asn Thr Val Asn Thr Met Arg Val Tyr Leu Leu305 310 315 320Tyr Ile Ala Met Tyr Glu Asn Lys Ser Ile Leu Val Phe Ile Thr Leu 325 330 335Phe Ser Gln Ile Leu 34047293PRTOryza sativa Indica 47Met Asp His Leu Trp Ala Ser Val Phe Gly Ile Leu Gly Asn Ile Val1 5 10 15Ser Phe Leu Val Phe Leu Ala Pro Met Pro Thr Phe Leu Arg Val Tyr 20 25 30Arg Lys Lys Ser Thr Glu Gly Phe Ser Ser Val Pro Tyr Val Val Ala 35 40 45Leu Phe Ser Cys Thr Leu Trp Ile Leu Tyr Ala Met Val Lys Thr Asn 50 55 60Ser Ser Pro Leu Leu Thr Ile Asn Ala Phe Gly Cys Val Val Glu Ala65 70 75 80Ala Tyr Ile Ala Val Tyr Leu Val Tyr Ala Pro Arg Pro Ala Arg Leu 85 90 95Arg Ala Leu Ala Ser Phe Leu Leu Leu Asn Val Ala Ala Phe Ser Leu 100 105 110Val Val Val Val Thr Val Ala Ala Val Val Gln Pro His Arg Val Arg 115 120 125Val Leu Gly Ser Ile Cys Leu Ala Phe Ser Met Ala Val Phe Val Ala 130 135 140Pro Met Ser Val Ile Met Val Val Ile Lys Thr Lys Ser Ala Glu Phe145 150 155 160Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala Val Ala Trp 165 170 175Phe Phe Tyr Gly Leu Phe Thr Asn Asp Leu Tyr Val Thr Leu Pro Asn 180 185 190Val Gly Gly Phe Phe Phe Gly Cys Val Gln Met Ala Leu Tyr Phe Lys 195 200 205Tyr Arg Lys Pro Asn Thr Ala Ala Gly Gly Val Met Ile Leu Pro Thr 210 215 220Thr Ala Ala Ala Ala Ala Val Asp Gly Ala Val Ala Glu Pro Ala Ala225 230 235 240Ala Ala Gln Gln Leu Ala Glu Glu Leu Glu Met Glu Leu Ala Ala Ala 245 250 255Gly Ala His Ala Val Ala Val Leu Pro Ala Ser Ala Leu Pro Val Leu 260 265 270Ala Glu Leu His Lys Met Glu Gln Glu Ile Gly Thr Pro Arg Lys Gly 275 280 285Ala Thr Lys Thr Val 29048284PRTPrunus persica 48Met Ala Ala Pro Asp Ala Phe Leu Leu Ala Ser Val Phe Gly Ile Leu1 5 10 15Gly Asn Ile Val Ala Phe Met Val Tyr Leu Ala Pro Leu Pro Thr Phe 20 25 30Tyr Arg Ile Phe Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Ser Val Ala Leu Phe Ser Ala Met Leu Met Leu Tyr Tyr Ala Phe 50 55 60Leu Lys Thr Asn Ala Phe Met Leu Ile Thr Ile Asn Ser Val Gly Cys65 70 75 80Ile Ile Glu Thr Ser Tyr Leu Val Met Tyr Met Ile Tyr Ala Pro Ala 85 90 95Lys Thr Arg Ile Tyr Thr Ala Lys Leu Leu Val Leu Phe Asn Val Gly 100 105 110Val Tyr Gly Val Ile Val Leu Ser Thr Tyr Leu Ile Pro Asn His Phe 115 120 125Leu Arg Ile Lys Val Val Gly Trp Ile Ser Val Val Phe Ser Val Cys 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Leu Val Ile Arg Thr Arg145 150 155 160Ser Val Glu Phe Met Ser Phe Pro Leu Ser Phe Cys Leu Thr Leu Cys 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Val Arg Asp Leu Phe Ile 180 185 190Ala Ala Pro Asn Ile Leu Gly Phe Ala Phe Gly Leu Ala Gln Met Ile 195 200 205Met Tyr Leu Met Phe Lys Asn Ser Lys Lys Ser Met Leu Pro Glu Phe 210 215 220Ser Leu Asn Gln Ile Pro Asn Val Val Ala Val Asn Asp Ile Val Ala225 230 235 240Ser Asp Ser Gln Leu Lys Thr Glu Asp Thr Lys Lys Ser Ser Glu Ala 245 250 255Glu Glu Asn Gln Ser Thr Glu Ser Met Thr Asn Asp Arg Arg Ala Gly 260 265 270Asp Ala Ala Ala Ala Glu Pro Asn Glu Ser Ile Val 275 28049293PRTPrunus persica 49Met Ala Ile Gln His Pro Leu Thr Leu Ser Phe Gly Leu Leu Gly Asn1 5 10 15Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Val Pro Thr Phe Tyr Thr 20 25 30Ile Tyr Lys Arg Lys Thr Ala Glu Gly Phe Gln Ala Leu Pro Tyr Val 35 40 45Ile Ala Leu Leu Ser Ser Met Leu Tyr Ile Tyr Tyr Ala Leu Leu Lys 50 55 60Glu Glu Phe Lys Glu Asp Ala Thr Phe Leu Ile Thr Ile Asn Ser Phe65 70 75 80Gly Cys Val Val Glu Thr Leu Tyr Ile Ser Leu Phe Leu Phe Tyr Ala 85 90 95Pro Lys Lys Ala Arg Ile Ser Thr Leu Thr Leu Val Phe Leu Leu Asn 100 105 110Leu Phe Gly Phe Gly Leu Met Met Leu Leu Thr His Phe Leu Ala Thr 115 120 125Gly Glu Met Arg Leu Lys Ile Val Gly Trp Ile Cys Leu Val Phe Ser 130 135 140Leu Ser Val Phe Val Ala Pro Leu Gly Val Leu Arg Arg Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Pro Leu Ser Phe Phe Leu Thr 165 170 175Leu Gly Ala Val Thr Trp Phe Phe Tyr Gly Leu Leu Ile Lys Asp Tyr 180 185 190Asn Ile Ala Phe Pro Asn Ile Leu Gly Phe Leu Phe Gly Ile Ala Gln 195 200 205Met Val Leu Tyr Ile Val Tyr Lys Asn Thr Lys Lys Val Leu Glu Glu 210 215 220Gln Pro Lys Val Gln Glu Leu Ser Glu His Ile Ile Asp Val Val Lys225 230 235 240Ile Ser Ser Leu Val Cys Pro Glu Leu Asn Pro Val Val Leu Gln Pro 245 250 255Thr Leu Asp Ile Thr Asn Asp Met Ile Glu Ala Val Gln Asn Ile Ile 260 265 270Val Met Ala Glu Lys Thr Glu Glu Ala Lys Glu Ala Met Asp Ile Asp 275 280 285Ala Ser Thr Lys Val 29050277PRTPrunus persica 50Met Gly Ala Leu Ala Asp Ser His His Pro Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Val Ile Ser Phe Leu Val Tyr Leu Ala Pro Val Pro 20 25 30Thr Phe Tyr Gly Ile Tyr Lys Lys Lys Ser Thr Gln Gly Phe Gln Ser 35 40 45Val Pro Tyr Leu Val Ala Leu Phe Ser Gly Met Leu Trp Phe Tyr Tyr 50 55 60Ala Leu Leu Lys Lys Asn Ala Met Leu Leu Ile Thr Ile Asn Ser Phe65 70 75 80Gly Thr Val Ile Glu Thr Ile Tyr Ile Val Met Phe Ile Phe Tyr Ala 85 90 95Pro Lys Asp Ala Arg Lys Phe Thr Leu Lys Leu Phe Gly Phe Met Asn 100 105 110Val Gly Leu Phe Cys Ser Ile Leu Val Leu Ser His Phe Ala Val Arg 115 120 125Ser Glu Tyr Arg Val Pro Val Leu Gly Trp Ile Asn Val Ala Ile Ser 130 135 140Val Ile Val Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Ile Arg145 150 155 160Thr Arg Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr 165 170 175Leu Ser Ala Val Met Trp Phe Ser Tyr Gly Leu Phe Leu Lys Asp Ile 180 185 190Cys Ile Ala Ile Pro Asn Val Leu Gly Phe Ile Leu Gly Leu Leu Gln 195 200 205Met Leu Leu Tyr Ala Ile Tyr Arg Asn Arg Lys Pro Ile Glu Asp Asp 210 215 220Glu Lys Lys Ile Pro Ala Ala Asp Gln His Val Lys Asn Val Val Gly225 230 235 240Leu Thr Thr Leu Ala Thr Ser Glu Val His Pro Val Asp Pro Pro Pro 245 250 255Arg Asp His Asp Lys Ser Val Glu Val Asp Ala Gly Ser His Thr Ala 260 265 270Ala Ala Ser Cys Ala 27551287PRTTriticum urartu 51Met Ala Glu Gly Leu Phe Ser Met Ala His Pro Trp Ala Ser Ala Phe1 5 10 15Gly Ile Leu Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Thr 20 25 30Pro Thr Phe Leu Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Ser 35 40 45Ala Val Pro Tyr Val Val Ala Leu Phe Ser Cys Met Leu Trp Ile Phe 50 55 60Tyr Ala Leu Val Lys Thr Asn Ser Ser Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Val Val Glu Ser Phe Tyr Ile Leu Leu Tyr Val Val Tyr 85 90 95Ala Pro Arg Asn Ala Arg His Arg Ala Leu Ala Phe Phe Leu Leu Leu 100 105 110Asp Val Ala Ala Phe Ser Leu Ile Val Val Val Thr Val Phe Leu Val 115 120 125Pro Gln Pro Ser Arg Val Lys Val Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Val Ile Phe Val Val Ile145 150 155 160Lys Thr Lys Ser Ala Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Phe Tyr Gly Leu Phe Thr Lys Asp 180 185 190Ile Tyr Val Thr Leu Pro Asn Val Gly Gly Phe Phe Phe Gly Val Ala 195 200 205Gln Met Thr Leu Tyr Phe Cys Tyr Arg Lys Pro Asp Thr Ser Ala Leu 210 215 220Val Leu Pro Thr Gly Ile His Asp Val Ser Thr Glu Ala Ala Ala Gln225 230 235 240Gln Glu Val Glu Leu Pro Glu Gly Thr His Pro Ala Ala Ala Met Leu 245 250 255Thr Val Ser Thr Leu Pro Met Leu Ala Glu Leu Gln Lys Met Glu Gln 260 265 270Glu Ile Ser Ser Pro Thr Pro Arg Lys Gly Tyr Ile Lys Ala Phe 275 280 28552345PRTTriticum urartu 52Met Ala Phe Leu Asn Met Glu Gln His Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Ile Ile Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Thr Pro Tyr Leu Val Thr Leu Phe Ser Cys Leu Leu Trp Met Tyr Tyr 50 55 60Ala Phe Leu Lys Ser Gly Ser Glu Leu Leu Leu Thr Ile Asn Ala Val65 70 75 80Gly Cys Val Ile Glu Ser Leu Tyr Ile Ala Met Tyr Leu Val Tyr Ala 85 90 95Pro Lys Ser Ala Arg Leu Leu Thr Ala Lys Leu Phe Ile Gly Leu Asp 100 105 110Val Gly Leu Phe Gly Leu Ile Ala Leu Val Thr Met Leu Ala Ser His 115 120 125Gly Pro Leu Arg Val Gln Val Val Gly Trp Ile Cys Val Ala Val Ala 130 135 140Leu Gly Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Leu Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Ile Trp Phe Ala Tyr Gly Ala Leu Lys Lys Asp Ile 180 185 190Phe Val Ala Met Pro Asn Val Leu Gly Phe Leu Phe Gly Val Ala Gln 195 200 205Met Ala Leu Tyr Met Ala Tyr Arg Asn Lys Lys Pro Ala Thr Val Ala 210 215 220Val Ile Asp Thr Thr Pro Arg Gln Arg Gln Arg Cys Thr Met Pro Val225 230 235 240Gly Arg Gln Thr Arg Arg His Lys Pro Phe Ala Thr Glu Lys Asn Arg 245 250 255Gly Ser Thr Asn Asp Ala Leu Lys Arg Gly Met Thr Pro Ser Ala Thr 260 265 270Asp Val Lys Ser Lys Arg Trp Thr Arg Ile Phe Thr Pro Asp Pro Trp 275 280 285His Leu Glu Gly Thr Leu Asn Asn Ala Pro Lys Arg Glu Ala Thr Leu 290 295 300Val Gly Ala Gly Val Thr Gly Ala Gly Ala Gln Ser Phe Arg Ser Asp305 310 315 320Pro His Lys Cys His Gln Arg Thr His Gly Cys Gln Pro Thr Pro Arg 325 330 335Arg Glu Val Ile Val Gly Asn Met Pro 340 34553290PRTTriticum urartu 53Met Gly Gly Leu Ser Met Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Ser Ser Leu Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Phe Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Val Lys Thr Gly Glu Gly Leu Leu Ile Ser Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Val Tyr Ile Val Met Tyr Leu Val Tyr Ala Pro 85 90 95Arg Lys Ala Lys Ile Phe Thr Ala Lys Ile Val Val Leu Leu Asn Ile 100 105 110Thr Gly Phe Gly Leu Ile Phe Leu Leu Thr Leu Phe Ala Phe His Gly 115 120 125Glu Thr Arg Val Val Ser Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Val Ala Pro Leu Ser Ile Ile Gly Arg Val Ile Lys Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Thr Leu Ser Leu Thr Leu Thr Leu 165 170 175Ser Ala Ile Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Val Ile

Gln Met 195 200 205Val Leu Tyr Val Phe Tyr Met Asn Lys Thr Pro Val Ala Ser Glu Val 210 215 220Lys Glu Gly Lys Glu Ala Trp Lys Ala Pro Ala Glu Asp His Val Val225 230 235 240Val Ile Asn Val Gly Lys Thr Asp Lys Gly Ser Cys Ala Glu Val Arg 245 250 255Pro Val Thr Glu Met Ala Ser Ala Val Asp Val Pro Arg Arg Cys Ala 260 265 270Ala Glu Ala Ala Ala Ala Pro Gly Val Asp Phe Ala Arg Ser Val Asp 275 280 285Val Val 29054310PRTAegilops tauschii 54Met Ala Phe Leu Asn Met Glu Gln His Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Ile Ile Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Val Gln Pro 35 40 45Thr Pro Tyr Leu Val Thr Leu Phe Ser Cys Leu Leu Trp Met Tyr Tyr 50 55 60Ala Phe Leu Lys Ser Gly Ser Glu Leu Leu Leu Thr Ile Asn Ala Val65 70 75 80Gly Cys Val Ile Glu Ser Leu Tyr Ile Ala Met Tyr Leu Val Tyr Ala 85 90 95Pro Lys Ser Ala Arg Leu Leu Thr Ala Lys Leu Phe Ile Gly Leu Asp 100 105 110Val Gly Leu Phe Gly Leu Ile Ala Leu Val Thr Met Leu Ala Ser Tyr 115 120 125Gly Pro Leu Arg Val Gln Val Val Gly Trp Ile Cys Val Ala Val Ala 130 135 140Leu Gly Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Leu Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Ile Trp Phe Ala Tyr Gly Ala Leu Lys Lys Asp Ile 180 185 190Phe Val Ala Met Pro Asn Val Leu Gly Leu Leu Phe Gly Val Ala Gln 195 200 205Met Ala Leu Tyr Met Ala Tyr Arg Asn Lys Lys Pro Ala Thr Val Val 210 215 220Leu Val His Glu Glu Met Lys Leu Pro Glu His Val Lys Glu Val Ala225 230 235 240Gly Gly Ala Lys Pro Gln Gly Gly Ala Pro Thr Glu Gly Arg Ile Ser 245 250 255Cys Gly Ala Glu Val His Pro Ile Asp Val Leu Pro Ala Val Ala Val 260 265 270Asp Glu Gln Ala Ala Gly Ala Ala Asp Glu Asp Val Ile Arg Asp Asp 275 280 285Gln Asn Met Leu Arg Pro Glu Gln Pro Val Val Ile Lys Pro Asp Val 290 295 300Ala Ile Val Val Gln Ala305 31055285PRTAegilops tauschii 55Met Ala Gly Leu Ser Leu Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Thr Ser Leu Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Phe Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Val Lys Thr Gly Glu Gly Leu Leu Ile Ser Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Val Tyr Ile Val Met Tyr Leu Val Tyr Ala Pro 85 90 95Arg Lys Ala Lys Ile Phe Thr Ala Lys Ile Val Val Leu Leu Asn Val 100 105 110Ala Gly Phe Gly Leu Ile Leu Leu Leu Thr Leu Phe Ala Phe His Gly 115 120 125Glu Thr Arg Val Ile Ser Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Val Ala Pro Leu Ser Ile Ile Gly Arg Val Ile Lys Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Met Ile Gln Met 195 200 205Val Leu Tyr Met Phe Tyr Met Asn Ala Thr Pro Val Val Ser Asp Leu 210 215 220Lys Glu Gly Lys Glu Gly Leu Lys Met Ser Ala Glu Glu His Val Val225 230 235 240Val Ile Asn Val Gly Lys Ser Glu Lys Ser Ser Gly Ala Val Val Arg 245 250 255Pro Val Thr Glu Met Val Lys Ala Val Pro Ala Ala Pro Gly Gln Gln 260 265 270Val Met Ala Leu Asp Ser Ala Arg Ser Val Asp Val Val 275 280 28556287PRTAegilops tauschii 56Met Gly Gly Leu Ser Leu Gln His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Arg Ser Lys Ser Thr Gln Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Leu Lys Ser Asp Glu Leu Leu Leu Ile Thr Ile Asn Ser Ala Gly65 70 75 80Cys Ile Ile Glu Thr Ile Tyr Ile Val Met Tyr Leu Ala Tyr Ala Pro 85 90 95Lys Gln Ala Lys Ile Phe Thr Ala Lys Ile Leu Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Leu Ile Leu Leu Leu Thr Leu Leu Leu Ala Gly Gly 115 120 125Glu Lys Arg Val Val Met Leu Gly Trp Val Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Ile Arg Leu Val Val Arg Thr145 150 155 160Arg Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Val 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Ala Phe Gly Val Ile Gln Met 195 200 205Gly Leu Tyr Ala Ile Tyr Cys Asn Ala Thr Pro Thr Leu Ala Pro Lys 210 215 220Glu Val Asp Arg Pro Leu Pro Glu His Val Ile Asn Val Ala Lys Leu225 230 235 240Gly Pro Thr Ala Thr Ile Glu Leu Asn Met Pro Ala Ala Val Gln Pro 245 250 255Pro Thr Lys Glu Asn Ile Val Ala Cys Ala Ser Gly Glu Thr Lys Glu 260 265 270Ile Ser Val Glu Lys Val Asp Met Ala Thr Asn Val Glu His Val 275 280 28557293PRTAegilops tauschii 57Met Gly Gly Leu Ser Leu Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Arg Ser Lys Ser Thr Gln Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Leu Lys Ser Asp Glu Leu Leu Leu Ile Thr Ile Asn Ser Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Ile Met Tyr Leu Thr Tyr Ala Pro 85 90 95Lys Gln Ala Lys Leu Phe Thr Ala Lys Ile Leu Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Leu Ile Leu Leu Leu Thr Leu Leu Leu Ala Gly Gly 115 120 125Glu Lys Arg Val Val Met Leu Gly Trp Val Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Val Ile Arg Leu Val Val Arg Thr145 150 155 160Arg Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Val 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Ala Phe Gly Val Ile Gln Met 195 200 205Gly Leu Tyr Ala Leu Tyr Cys Asn Ala Met Pro Arg Gln Ala Pro Lys 210 215 220Glu Val Asp Asp Pro Met Ser Asp His Gly Ala Ala Val Lys Ala Pro225 230 235 240Glu His Val Val Asn Ile Ser Lys Leu Ser Pro Ala Ala Gly Ile Glu 245 250 255Leu Asn Thr Thr Val Asn Ala Glu Pro Pro Leu Lys Ser Leu Gly Val 260 265 270Ala Cys Ala Asn Glu Glu Thr Ile Gly Val Ser Val Asp Lys Ala Thr 275 280 285His Ile Glu Gln Val 29058290PRTAegilops tauschii 58Met Gly Gly Leu Ser Met Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Ser Ser Leu Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Phe Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Val Lys Thr Gly Glu Gly Leu Leu Ile Ser Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Val Tyr Ile Val Met Tyr Leu Val Tyr Ala Asp 85 90 95Arg Lys Ala Lys Ile Phe Thr Ala Lys Ile Val Val Leu Leu Asn Ile 100 105 110Ala Gly Phe Gly Leu Ile Phe Leu Leu Thr Leu Phe Ala Phe His Gly 115 120 125Glu Thr Arg Val Val Thr Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Val Ala Pro Leu Ser Ile Ile Gly Arg Val Ile Lys Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Thr Leu Ser Leu Thr Leu Thr Leu 165 170 175Ser Ala Ile Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Val Ile Gln Met 195 200 205Val Leu Tyr Val Phe Tyr Met Asn Lys Thr Pro Val Ala Ser Gln Val 210 215 220Lys Glu Gly Lys Glu Ala Trp Lys Ala Pro Ala Glu Asp His Val Val225 230 235 240Val Ile Asn Val Gly Lys Ala Asp Lys Ser Ser Cys Ala Glu Val Arg 245 250 255Pro Val Thr Glu Met Ala Gly Ala Val Asp Val Pro Arg Arg Cys Ala 260 265 270Ala Glu Ala Ala Ala Ala Pro Gly Val Asp Phe Ala Arg Ser Val Asn 275 280 285Val Val 29059237PRTAegilops tauschii 59Met Gly Ala Val Gly Ser Pro Leu Val Phe Ala Val Gly Ile Leu Gly1 5 10 15Asn Ile Leu Ser Phe Leu Val Ile Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Tyr Lys Arg Lys Ser Thr Glu Ser Phe Gln Ser Val Pro Tyr 35 40 45Ala Met Ala Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Thr Lys Asp Leu Leu Leu Leu Thr Ile Asn Thr Val Gly Cys Val Val65 70 75 80Glu Ser Ala Tyr Leu Ala Ile Tyr Leu Ala Tyr Ala Pro Lys Gln Ala 85 90 95Arg Thr Phe Thr Ala Lys Leu Val Cys Ile Met Asn Val Ala Leu Tyr 100 105 110Gly Ala Met Val Cys Val Leu Gln Leu Leu Val Lys Asp Gly Glu Ser 115 120 125Arg Val Thr Ile Ala Gly Gly Ile Gly Ser Ala Phe Ala Leu Ala Val 130 135 140Phe Val Ala Pro Leu Ala Ile Ile Arg Gln Val Ile Arg Thr Lys Ser145 150 155 160Val Glu Phe Leu Pro Phe Trp Leu Ser Phe Phe Leu Thr Ile Ser Ala 165 170 175Val Val Trp Phe Phe Tyr Gly Leu Leu Met Lys Asp Phe Phe Val Ala 180 185 190Thr Pro Asn Val Leu Gly Leu Leu Phe Gly Leu Ala Gln Met Ser Leu 195 200 205His Leu Val Tyr Lys Asn Pro Lys Lys Lys Gly Ala Val Ser Glu Val 210 215 220Gln Tyr Ser Ile Gly Leu Leu Val Trp Ala Arg Leu Ile225 230 23560275PRTAegilops tauschii 60Met Ala Gly Gly Leu Phe Asp Met Ser His Pro Ala Ser Ala Leu Ala1 5 10 15Gly Ile Ala Gly Asn Ile Val Ser Phe Phe Val Phe Leu Ala Pro Met 20 25 30Ala Thr Phe Leu Gln Ile Tyr Arg Lys Lys Thr Thr Gly Gly Phe Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Cys Ser Leu Leu Ile Phe 50 55 60Tyr Ala Leu Leu Lys Thr Asp Ser Pro Leu Leu Leu Thr Ile Asn Ser65 70 75 80Phe Gly Cys Cys Ile Glu Thr Val Tyr Ile Val Ala Tyr Leu Val Tyr 85 90 95Ala Pro Pro Arg Ala Arg Leu Arg Thr Leu Ala Tyr Phe Phe Val Leu 100 105 110Asp Val Ala Ala Phe Gly Leu Val Leu Val Val Thr Met Tyr Ala Phe 115 120 125Ala Pro Ala His Arg Val Lys Phe Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Ile Ile Val Lys Val Ile145 150 155 160Lys Thr Lys Ser Val Glu Phe Leu Pro Val Gly Leu Ser Phe Cys Leu 165 170 175Val Leu Ser Ala Val Ala Trp Phe Cys Tyr Gly Leu Phe Thr Lys Asp 180 185 190Pro Phe Val Met Tyr Pro Asn Val Gly Gly Phe Phe Phe Ser Cys Val 195 200 205Gln Ile Gly Leu Tyr Cys Trp Tyr Arg Lys Pro Ser Asn Ala Val Leu 210 215 220Pro Thr Thr Thr Ala Asp Ala Gly Asn Gly Asn Gly Gly Pro Thr Pro225 230 235 240Ala Ala Gly Ala Glu Gln Gln Thr Val Ile Asp Val Lys Asp Ala Ala 245 250 255Arg Ala Ala Glu Val Asp Gln Pro Glu Val Ile Glu Ile Val Pro Ala 260 265 270Pro Ala Val 27561290PRTCapsella rubella 61Met Ala Ile Ser Gln Ala Val Leu Ala Thr Val Phe Gly Ile Leu Gly1 5 10 15Asn Val Ile Ser Phe Phe Val Cys Leu Ala Pro Ile Pro Thr Phe Ile 20 25 30Arg Ile Tyr Lys Arg Lys Ser Ser Glu Gly Tyr Gln Ser Val Pro Tyr 35 40 45Val Ile Ser Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr Ala Met Ile 50 55 60Lys Lys Asp Ala Val Met Leu Ile Thr Ile Asn Ser Phe Ala Phe Val65 70 75 80Ile Gln Ile Val Tyr Ile Ser Leu Phe Phe Phe Tyr Ala Pro Lys Lys 85 90 95Asp Lys Ile Leu Thr Val Lys Phe Val Leu Phe Val Asp Val Phe Ala 100 105 110Phe Gly Leu Ile Phe Phe Ser Thr Tyr Phe Pro Ile His Gly Asn Lys 115 120 125Arg Val Gln Val Leu Gly Tyr Ile Cys Met Val Phe Ala Leu Ser Val 130 135 140Phe Val Ala Pro Leu Gly Ile Ile Arg Lys Val Ile Lys Thr Lys Ser145 150 155 160Ala Glu Phe Met Pro Phe Gly Leu Ser Phe Phe Leu Thr Leu Ser Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Lys Asn Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Ile Phe Gly Val Leu Gln Met Val Leu 195 200 205Phe Val Ile Tyr Lys Lys Pro Gly Thr Lys Val Leu Glu Pro Ser Val 210 215 220Ile Lys Leu Gln Asp Ile Ser Glu His Val Val Asp Val Val Arg Leu225 230 235 240Ser Ser Met Val Cys Asn Ser Gln Met Arg Thr Leu Val Pro Gln Asp 245 250 255Ser Ala Asp Met Glu Asp Thr Ile Asp Ile Glu Glu Lys Met Lys Gly 260 265 270Asp Ile Glu Lys Asn Lys Asp Asn Asn Lys Glu Ala Phe Leu Ile Ser 275 280 285Lys Asn 29062292PRTCapsella rubella 62Met Ala Leu Thr His Asn Val Trp Ala Phe Val Phe Gly Ile Met Gly1 5 10 15Asn Ile Ile Ser Phe Val Val Phe Leu Ala Pro Val Pro Thr Phe Ile 20 25 30Arg Ile Cys Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Leu Ser Ala Ile Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Met Gln 50 55 60Lys Asp Gly Ser Gly Phe Leu Leu Ile Thr Ile Asn Ala Val Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Ile Val Leu Phe Val Thr Tyr Ala Asn Lys 85

90 95Lys Thr Arg Ile Ser Thr Leu Lys Val Leu Gly Leu Leu Asn Phe Leu 100 105 110Gly Phe Ala Ala Ile Val Leu Val Cys Glu Leu Leu Thr Lys Gly Ser 115 120 125Thr Arg Ala Lys Val Leu Gly Gly Ile Cys Val Gly Phe Ser Val Ser 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Leu Val Val Arg Thr Arg145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Leu Ser 165 170 175Ala Val Thr Trp Leu Phe Tyr Gly Leu Ala Ile Lys Asp Phe Tyr Val 180 185 190Ala Leu Pro Asn Val Leu Gly Ala Phe Leu Gly Ala Val Gln Met Ile 195 200 205Leu Tyr Ile Val Phe Lys Tyr Tyr Met Thr Pro Val Ala Glu Lys Thr 210 215 220Asp Lys Ser Lys Ala Val Ser Ser Asp His Ser Ile Asp Ile Ala Lys225 230 235 240Leu Thr Thr Val Ile Pro Gly Ser Thr Val His Glu Pro Pro Ala Val 245 250 255His Asn Val Pro Glu Thr Gln Ile Gln Val Thr Glu Val Lys Ser Gln 260 265 270Asn Met Thr Glu Pro Asn Asp Gln Thr Thr Ser Lys Asp Val Gln Asn 275 280 285Gln Asn Gln Val 29063278PRTCapsella rubella 63Met Val Leu Ala His Asn Val Leu Ala Val Thr Phe Gly Val Met Gly1 5 10 15Asn Ile Ile Ser Phe Ile Val Phe Leu Ala Pro Val Pro Thr Phe Val 20 25 30Arg Ile Cys Lys Lys Lys Ser Thr Glu Gly Phe Glu Ser Leu Pro Tyr 35 40 45Val Ser Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Met Gln 50 55 60Lys Asp Gly Ala Gly Phe Leu Leu Ile Thr Ile Asn Ala Val Gly Cys65 70 75 80Phe Ile Glu Thr Ile Tyr Ile Ile Leu Phe Leu Thr Tyr Ala Asn Lys 85 90 95Lys Ala Arg Ile Ser Thr Leu Lys Val Leu Gly Leu Leu Asn Phe Leu 100 105 110Gly Phe Ala Ala Ile Ile Leu Val Cys Glu Leu Leu Thr Lys Gly Ser 115 120 125Asn Arg Glu Lys Val Leu Gly Gly Ile Cys Val Gly Phe Ser Val Cys 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Val Val Ile Arg Thr Lys145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Leu Ser 165 170 175Ala Ile Thr Trp Leu Phe Tyr Gly Leu Ala Ile Lys Asp Phe Tyr Val 180 185 190Ala Leu Pro Asn Ile Met Gly Ala Phe Leu Gly Ala Val Gln Met Ile 195 200 205Leu Tyr Val Ile Phe Lys Tyr Tyr Lys Ser Pro Val Val Val Asp Glu 210 215 220Thr Glu Lys Pro Lys Thr Val Ser Ala Asp His Ser Ile Asn Met Ala225 230 235 240Lys Leu Ser Ser Thr Pro Ala Ser Gly Glu Leu Thr Val His Ser Ser 245 250 255Gln Thr Asn Pro Val Gln Thr Gly Ala Gly Asp Leu Glu Asp Gln Met 260 265 270Asp Lys Lys Ile Ser Asn 27564287PRTCapsella rubella 64Met Ala Leu Phe Asp Thr His Asn Thr Trp Ala Phe Val Phe Gly Leu1 5 10 15Leu Gly Asn Leu Ile Ser Phe Ala Val Phe Leu Ser Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Cys Lys Lys Lys Thr Thr Glu Gly Phe Gln Ser Ile 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr Ala 50 55 60Thr Gln Lys Lys Asp Val Phe Leu Leu Val Thr Ile Asn Ser Phe Gly65 70 75 80Cys Phe Ile Glu Thr Ile Tyr Ile Ser Ile Phe Leu Ala Phe Ala Thr 85 90 95Lys Asn Ala Arg Met Leu Thr Val Lys Leu Leu Leu Leu Met Asn Val 100 105 110Gly Gly Phe Cys Ala Ile Leu Leu Leu Cys Gln Phe Leu Ala Lys Gly 115 120 125Ala Thr Arg Ala Lys Ile Ile Gly Gly Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Thr Val Ile Lys Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Ile 165 170 175Ser Ala Val Ile Trp Leu Leu Tyr Gly Leu Ala Leu Lys Asp Ile Tyr 180 185 190Val Ala Phe Pro Asn Val Ile Gly Phe Ala Leu Gly Ala Leu Gln Met 195 200 205Ile Leu Tyr Val Val Tyr Lys Tyr Cys Lys Thr Ser Ser Asp Leu Val 210 215 220Glu Lys Glu Leu Glu Asn Ala Lys Leu Pro Glu Val Ser Ile Asp Met225 230 235 240Leu Lys Leu Gly Gly Ala Ala Glu Pro Ala Cys Gly Ile Thr Val Val 245 250 255Arg Ser Val Asn Met Cys Asn Cys Asn Asp Arg Arg Val Glu Ile Glu 260 265 270Asn Gly His Gly Leu Val Arg Asn Ser Ala Thr Ala Ala Ala Thr 275 280 28565286PRTCapsella rubella 65Met Gly Val Val Met Asn His His Leu Leu Thr Ile Ile Phe Gly Ile1 5 10 15Leu Gly Asn Ala Val Ser Phe Leu Val Leu Val Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Lys Lys Ser Thr Glu Ser Phe Gln Ser Leu 35 40 45Pro Tyr Gln Val Ser Leu Phe Ser Cys Met Leu Trp Leu Tyr Tyr Ala 50 55 60Leu Ile Lys Lys Asn Ala Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly65 70 75 80Cys Val Val Gln Thr Ile Tyr Ile Ala Met Phe Leu Ala Tyr Ala Thr 85 90 95Arg Asp Lys Arg Ile Ser Ala Met Lys Leu Phe Ile Ala Ile Asn Val 100 105 110Val Phe Phe Ser Leu Ile Leu Leu Val Thr His Phe Val Val Lys Thr 115 120 125Pro Thr Leu Gln Val Ser Val Leu Gly Trp Ile Cys Val Ala Ile Ser 130 135 140Val Ala Val Phe Ala Ala Pro Leu Met Ile Val Ala Arg Val Val Lys145 150 155 160Thr Lys Ser Val Glu Tyr Met Pro Phe Thr Leu Ser Phe Phe Leu Thr 165 170 175Ile Ser Ala Val Met Trp Phe Gly Tyr Gly Leu Phe Leu Asn Asp Ile 180 185 190Cys Ile Ala Ile Pro Asn Val Val Gly Phe Val Leu Gly Met Leu Gln 195 200 205Met Val Leu Tyr Cys Val Tyr Arg Asn Ala Ser Glu Lys Pro Glu Ile 210 215 220Glu Lys Lys Ile Asn Leu Ser Glu Gln Gln Leu Lys Ser Ile Val Val225 230 235 240Met Ser Pro Leu Gly Val Ser Glu Val His Pro Val Val Thr Ala Ser 245 250 255Val Gly Pro Pro Ser Asp Ala Val His His Glu Glu Pro Ser Asn Gly 260 265 270Asn Arg Lys Cys His Val Glu Thr Ser Arg Pro Glu Asn Val 275 280 28566291PRTCapsella rubella 66Met Thr Leu Phe Asn Thr Glu Asn Thr Trp Ala Phe Val Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Ala Val Phe Leu Ser Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Trp Lys Lys Lys Thr Thr Glu Gly Phe Gln Ser Ile 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Thr Leu Trp Leu Tyr Tyr Ala 50 55 60Thr Gln Lys Lys Asp Val Phe Leu Leu Val Thr Ile Asn Ala Phe Gly65 70 75 80Cys Phe Ile Glu Thr Ile Tyr Ile Ser Met Phe Leu Ala Tyr Ala Pro 85 90 95Lys Pro Ala Arg Met Leu Thr Val Lys Ile Leu Leu Leu Met Asn Phe 100 105 110Gly Gly Phe Cys Leu Ile Leu Leu Leu Cys Gln Leu Leu Leu Lys Gly 115 120 125Ala Thr Arg Ala Lys Ile Ile Gly Gly Ile Cys Val Gly Phe Ser Val 130 135 140Cys Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Thr Val Ile Lys Thr145 150 155 160Arg Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Thr Leu Thr Ile 165 170 175Ser Ala Ile Ile Trp Phe Leu Tyr Gly Leu Ala Leu Lys Asp Ile Tyr 180 185 190Val Ala Phe Pro Asn Val Leu Gly Phe Ala Leu Gly Ala Leu Gln Met 195 200 205Ile Leu Tyr Val Val Tyr Lys Tyr Cys Lys Thr Ser Pro His Pro His 210 215 220Leu Gly Glu Lys Glu Val Glu Ala Ala Lys Leu Pro Glu Val Val Thr225 230 235 240Leu Asp Met Leu Lys Leu Gly Ala Val Ala Ser Pro Asp Pro Gly His 245 250 255Val Val Arg Gln Cys Asn Lys Cys Thr Cys Gly Asn Asp Arg Arg Val 260 265 270Ala Glu Ile Glu Asp Gly Arg Gln Thr Pro Arg Asn Ser Ser Ser Ala 275 280 285Ala Ala Thr 29067260PRTCapsella rubella 67Met Val Phe Ile Lys Val His Gln Leu Ala Phe Phe Phe Gly Leu Met1 5 10 15Gly Asn Ile Val Ser Phe Gly Val Phe Leu Ser Pro Val Pro Thr Phe 20 25 30Tyr Gly Ile Tyr Lys Lys Lys Ser Ser Lys Gly Phe Gln Ser Ile Pro 35 40 45Tyr Ile Cys Ala Leu Ala Ser Ala Thr Leu Leu Leu Tyr Tyr Gly Ile 50 55 60Met Lys Thr His Ala Tyr Leu Ile Ile Ser Ile Asn Thr Phe Gly Cys65 70 75 80Phe Ile Glu Ile Thr Tyr Leu Phe Leu Tyr Ile Phe Tyr Ala Pro Arg 85 90 95Glu Ala Arg Ile Phe Thr Leu Lys Leu Ile Val Ile Cys Asn Ile Gly 100 105 110Gly Leu Gly Leu Leu Ile Leu Leu Val Asn Leu Leu Val Pro Lys Pro 115 120 125His Arg Val Ser Thr Val Gly Trp Val Cys Ala Ala Tyr Ser Leu Ala 130 135 140Val Phe Ala Ser Pro Leu Ser Val Met Arg Lys Val Ile Lys Thr Lys145 150 155 160Ser Val Glu Tyr Met Pro Phe Leu Leu Ser Leu Ser Leu Thr Leu Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Ile Lys Asp Lys Phe Ile 180 185 190Ala Met Pro Asn Ile Leu Gly Phe Leu Phe Gly Val Ala Gln Met Ile 195 200 205Leu Tyr Met Met Tyr Gln Gly Ser Thr Lys Thr Asp Leu Pro Thr Glu 210 215 220His Gln Leu Gly Asn Lys Thr Asp Val Asn Glu Ile Ala Val Val Ala225 230 235 240Val Glu Leu Pro Asp Ala Arg Leu Asp Asn Val Glu Gly Ser Ala Arg 245 250 255Pro Ala Met Lys 26068344PRTZea mays 68Met Ala Gly Leu Ser Leu Gln His Pro Met Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Cys Arg Ile Tyr Arg Asn Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Leu Lys Ser Asn Glu Phe Leu Leu Ile Thr Ile Asn Ser Ala Gly65 70 75 80Cys Val Ile Glu Thr Leu Tyr Ile Ala Thr Tyr Leu Leu Tyr Ala Pro 85 90 95Asn Lys Ala Lys Leu Phe Thr Ala Lys Ile Leu Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Leu Ile Leu Leu Leu Thr Leu Leu Leu Ser Ala Gly 115 120 125Pro His Arg Val Val Val Leu Gly Trp Val Cys Val Ala Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Ile Arg Gln Val Val Arg Thr145 150 155 160Arg Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Ser Leu Thr Ala 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Val Leu Gly Phe Thr Phe Gly Val Val Gln Met 195 200 205Gly Met Tyr Ala Leu Tyr Arg Asn Ala Thr Pro Arg Val Pro Ala Ala 210 215 220Lys Glu Ala Ala Ala Ala Ala Asp Asp Gly Asn Thr Phe Asn Phe Lys225 230 235 240Ala Pro Gly Glu His Val Val Thr Ile Ala Lys Leu Thr Ala Ala Ala 245 250 255Pro Ala Thr Ala Ala Glu Leu Ile Ile Lys Ala Arg Asp Asp Ala Gln 260 265 270His Pro Pro Glu Glu Glu Ala Ala Ala Ala Lys Ala Ala Pro Ala Lys 275 280 285Ser Lys Leu Leu Ile Pro Leu Pro Glu His Ala Tyr Ala Cys Met Cys 290 295 300Ile Ile Arg Ser Gly Ser His His Lys Leu Gly Arg Ala Cys Leu Leu305 310 315 320Gly Thr Ser Thr Arg Pro Pro Ala Cys Leu Pro Ala Arg Met Ile Gln 325 330 335Ser Ser Cys Tyr Ile Arg Lys Gly 34069295PRTZea mays 69Met Ala Gly Leu Ser Leu Gln His Pro Trp Ala Phe Thr Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Thr Phe Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Ile Lys Ser Asn Glu Thr Phe Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Val Tyr Val Val Met Tyr Phe Val Tyr Ala Thr 85 90 95Lys Lys Gly Arg Met Phe Thr Ala Lys Ile Met Leu Leu Leu Asn Val 100 105 110Gly Ala Phe Gly Ala Ile Leu Leu Leu Thr Leu Leu Leu Phe Lys Gly 115 120 125Asp Lys Arg Val Val Met Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Met Arg Arg Val Ile Gln Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Val Val Gln Met 195 200 205Val Leu Tyr Val Val Tyr Met Asn Lys Thr Pro Leu Pro Val Ala Asp 210 215 220Gly Lys Ala Ala Gly Lys Leu Pro Ser Ala Ala Asp Glu His Val Val225 230 235 240Val Asn Val Thr Lys Leu Ser Pro Gly Arg Leu Pro Pro Val Thr Gln 245 250 255Met Ala Ala Val Pro Thr Lys Ser Cys Ala Thr Glu Ala Ala Ala Pro 260 265 270Ala Thr Leu Pro Ser Arg Asp Val Val Asp Val Leu Val Asn Arg His 275 280 285Ser Pro Ala Val His Val Thr 290 29570301PRTZea mays 70Met Ala Gly Leu Ser Leu Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Thr Phe Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Ile Lys Ser Asn Glu Thr Phe Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Val Met Tyr Phe Val Tyr Ala Pro 85 90 95Lys Lys Ala Lys Leu Phe Thr Ala Lys Ile Met Ala Leu Leu Asn Gly 100 105 110Gly Val Phe Gly Val Ile Leu Leu Leu Thr Leu Leu Leu Phe Lys Gly 115 120 125Ser Lys Arg Val Val Leu Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Met Arg Arg Val Ile Gln Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Val Leu Gly Phe Ile Phe Gly Val Val Gln Met 195 200 205Val Leu Tyr Val Phe Tyr Met Asn Lys Thr Pro Val Ala Ala Ala Val 210

215 220Gly Lys Asp Ala Gly Lys Leu Pro Ser Ala Ala Asp Glu His Val Leu225 230 235 240Val Asn Ile Ala Lys Leu Asn Pro Ala Leu Pro Glu Arg Thr Ser Gly 245 250 255Met His Pro Val Thr Gln Met Ala Ala Val Pro Ala Arg Ser Cys Ala 260 265 270Ala Glu Ala Ile Ala Pro Ala Met Leu Pro Asn Arg Asp Val Val Asp 275 280 285Val Phe Val Ser Arg His Ser Pro Ala Val His Val Val 290 295 30071310PRTZea mays 71Met Ala Gly Gly Leu Phe Ser Met Ala His Pro Ala Val Thr Leu Ser1 5 10 15Gly Ile Ala Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Val 20 25 30Ala Thr Phe Leu Gln Val Tyr Arg Lys Lys Ser Thr Gly Gly Phe Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Ser Val Leu Trp Ile Phe 50 55 60Tyr Ala Leu Val Lys Thr Asn Ser Arg Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Gly Val Glu Ala Ala Tyr Ile Val Leu Tyr Leu Ala Tyr 85 90 95Ala Pro Arg Arg Ala Arg Leu Arg Thr Leu Ala Tyr Phe Phe Leu Leu 100 105 110Asp Val Ala Ala Phe Ala Leu Val Val Ala Val Thr Leu Phe Ala Val 115 120 125Arg Glu Pro His Arg Val Lys Phe Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Ile Ile Val Lys Val Val145 150 155 160Lys Thr Lys Ser Val Glu Phe Leu Pro Ile Ser Leu Ser Phe Cys Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Cys Tyr Gly Leu Phe Thr Lys Asp 180 185 190Pro Phe Val Met Tyr Pro Asn Val Gly Gly Phe Phe Phe Ser Cys Val 195 200 205Gln Met Gly Leu Tyr Phe Trp Tyr Arg Lys Pro Arg Pro Ala Ala Lys 210 215 220Asn Asn Ala Val Leu Pro Thr Thr Thr Asp Gly Gly Asn Ala Val Gln225 230 235 240Val Gln Gly Gln Val Ile Glu Leu Ala Pro Asn Thr Val Ala Ile Leu 245 250 255Ser Val Ser Pro Ile Pro Ile Val Gly Val His Lys Ile Glu Val Val 260 265 270Glu Gln Gln His Lys Glu Ala Ala Val Ala Ala Glu Thr Arg Arg Met 275 280 285Ala Ala Ala Asn Pro Asp Gly Ala Met Pro Glu Val Ile Glu Ile Val 290 295 300Pro Ala Ala Ala Ala Val305 31072307PRTZea mays 72Met Ala Phe Leu Asn Met Glu Gln Gln Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Ile Val Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Asn Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Thr Pro Tyr Val Val Thr Leu Phe Ser Cys Met Leu Trp Ile Leu Tyr 50 55 60Ala Leu Leu Lys Pro Gly Ala Glu Leu Leu Val Thr Ile Asn Gly Val65 70 75 80Gly Cys Val Val Glu Thr Val Tyr Leu Ala Met Tyr Leu Val Tyr Ala 85 90 95Pro Lys Ala Ala Arg Val Leu Ala Ala Lys Met Leu Leu Gly Leu Asn 100 105 110Val Ala Val Phe Gly Leu Val Ala Leu Val Thr Met Leu Leu Ser Asp 115 120 125Ala Gly Leu Arg Val His Val Leu Gly Trp Ile Cys Val Ser Val Ser 130 135 140Leu Ser Val Phe Ala Ala Pro Leu Ser Ile Met Arg Gln Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Ile Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Val Trp Phe Ala Tyr Gly Ala Leu Lys Lys Asp Val 180 185 190Phe Val Ala Phe Pro Asn Val Leu Gly Phe Val Phe Gly Leu Ala Gln 195 200 205Met Ala Leu Tyr Met Ala Tyr Ser Arg Asn Arg Lys Pro Ala Ala Ala 210 215 220Leu Val Ile Leu Pro Glu Gln Ser Lys Glu Glu Ala Ala Glu Gly Lys225 230 235 240Ala Ser Cys Gly Gly Ala Glu Val His Pro Ile Asp Ile Ala Glu Val 245 250 255His Asp Leu Gln Ala Val Val Val Asp Val Asp Val Glu Pro Val Thr 260 265 270Tyr Ala Ala Ala Ser Gly Met Val Asp Gly Ser Val Gly Arg Pro Arg 275 280 285Ala Pro Glu Gln Leu Val Ile Lys Pro Asp Met Val Thr Val Ile Ala 290 295 300Ala Glu Ala30573260PRTGlycine max 73Met Ala Ile Asn His Glu Thr Trp Ala Phe Val Phe Gly Leu Leu Gly1 5 10 15Asn Val Ile Ser Phe Met Val Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Gln Ile Tyr Lys Lys Lys Ser Thr Glu Glu Phe Gln Ser Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Leu Val 50 55 60Lys Lys Asp Ala Ser Leu Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Leu Ala Ile Phe Leu Ile Tyr Ala Pro Ser 85 90 95Lys Thr Arg Leu Trp Thr Ile Lys Leu Leu Leu Met Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ser Thr Leu Tyr Leu Thr Thr Gly Ser 115 120 125Lys Arg Leu Thr Val Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Ser 130 135 140Val Phe Ala Ala Pro Leu Cys Ile Ile Lys Arg Val Ile Lys Thr Lys145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Tyr Tyr Val 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Leu Phe Ser Ile Ile Gln Met Val 195 200 205Leu Tyr Leu Ile Tyr Arg Asn Ala Lys Thr Pro Asp Leu Pro Met Lys 210 215 220Leu Gln Glu Leu Asn Ser His Thr Ile Asp Val Gly Lys Leu Ser Arg225 230 235 240Met Glu Pro Ser Glu Pro Asn His Val Thr Lys Asn Gly Thr Leu Thr 245 250 255Glu Arg Glu Ile 26074268PRTGlycine max 74Met Ala Ile Ser His Ser Thr Leu Ala Phe Ala Phe Gly Met Leu Gly1 5 10 15Asn Val Ile Ser Phe Leu Val Phe Leu Ala Pro Ile Thr Thr Phe Tyr 20 25 30Arg Ile Phe Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Lys Lys Asp Ala Met Leu Leu Leu Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Ile Glu Val Ile Tyr Ile Ile Leu Tyr Ile Thr Tyr Ala Thr Arg Asp 85 90 95Ala Arg Asn Leu Thr Leu Lys Leu Phe Phe Ala Met Asn Val Gly Ala 100 105 110Phe Ala Leu Ile Leu Leu Val Thr His Phe Ala Val His Gly Ser Leu 115 120 125Arg Val Gln Val Leu Gly Trp Ile Cys Val Ser Leu Ser Ile Ser Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Val Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Asn Leu Ser Phe Thr Leu Thr Leu Ser Ala 165 170 175Ile Met Trp Phe Gly Tyr Gly Leu Phe Leu Lys Asp Ile Cys Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Ala Leu Gly Leu Leu Gln Met Leu Leu 195 200 205Tyr Ala Ile Tyr Arg Asn Gly Asn Lys Lys Val Asp Lys Ile Met Glu 210 215 220Lys Lys Ala Pro Leu Glu Pro Leu Lys Thr Val Val Ile Glu Thr Gly225 230 235 240Leu Glu Glu Lys Gln Gln Gly Lys Lys Ser Lys Glu Asn Ser Glu Glu 245 250 255Lys Glu Lys Ser Asp Glu Pro Asn Asp Cys Ala Val 260 26575257PRTGlycine max 75Met Thr Met His Arg Glu Ser Trp Ala Phe Val Phe Gly Val Met Gly1 5 10 15Asn Ile Ile Ser Phe Gly Val Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Gln Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Val 50 55 60Lys Arg Glu Ala Ala Leu Leu Leu Ile Thr Ile Asn Thr Phe Gly Ile65 70 75 80Val Val Glu Ser Ile Tyr Leu Ala Ile Phe Leu Leu Tyr Ala Pro Arg 85 90 95Lys Pro Arg Leu Thr Thr Ile Lys Leu Leu Leu Leu Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ser Thr Leu Tyr Leu Ser Lys Gly Ala 115 120 125Lys Arg Leu Ala Ile Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Ser 130 135 140Val Phe Ala Ala Pro Leu Phe Ile Ile Arg Arg Val Ile Lys Thr Arg145 150 155 160Ser Val Glu Tyr Met Pro Phe Thr Leu Ser Met Phe Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Arg Asp Tyr Tyr Val 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Val Phe Gly Ile Ile Gln Met Gly 195 200 205Met Tyr Leu Met Tyr Arg Asn Ala Thr Pro Val Ala Leu Glu Glu Pro 210 215 220Val Lys Ala Gln Glu Leu Asn Gly His Ile Ile Asp Val Gly Lys Met225 230 235 240Gly Thr Met Glu Pro Asn His Ala Ala Thr Ala Gly Ala Val Gly Lys 245 250 255Val76258PRTGlycine max 76Met Ala Ile Asn His Glu Thr Trp Ala Phe Ile Phe Gly Leu Leu Gly1 5 10 15Asn Val Ile Ser Phe Met Val Phe Leu Ala Ser Leu Pro Thr Leu Tyr 20 25 30Gln Ile Tyr Lys Lys Lys Ser Thr Asp Gly Phe Gln Ser Leu Pro Tyr 35 40 45Ile Val Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Leu Val 50 55 60Lys Lys Asp Ala Ser Leu Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Leu Ala Ile Phe Leu Ile Tyr Ala Pro Ser 85 90 95Lys Thr Arg Leu Trp Thr Ile Lys Leu Leu Leu Met Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ser Thr Leu Tyr Leu Thr Thr Gly Ser 115 120 125Lys Arg Leu Ser Val Ile Gly Trp Ile Cys Leu Val Leu Asn Ile Ser 130 135 140Val Phe Ala Ala Pro Leu Cys Ile Met Lys Arg Val Ile Lys Thr Lys145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Tyr Tyr Ile 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Leu Phe Gly Ile Ile Gln Met Val 195 200 205Leu Tyr Leu Ile Tyr Arg Asn Ala Lys Pro Gln Gly Leu Glu Glu Pro 210 215 220Thr Lys Val Gln Glu Leu Asn Gly His Ile Ile Asp Val Val Lys Pro225 230 235 240Asn His Val Thr Lys Asn Gly Pro Val Pro Val Ile Glu Thr Ala Ser 245 250 255Asn Val77289PRTVitis vinifera 77Met Ala Met Ala Met Ala Asn His His Thr Leu Gly Leu Ile Phe Gly1 5 10 15Ile Leu Gly Asn Ile Ile Ser Phe Leu Val Tyr Phe Ala Pro Ala Pro 20 25 30Thr Phe Tyr Arg Ile Tyr Lys Arg Lys Ser Ala Glu Gly Phe His Ser 35 40 45Leu Pro Tyr Ile Val Ala Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr 50 55 60Ala Leu Leu Lys Lys Asp Ala Phe Leu Leu Ile Thr Ile Asn Ser Phe65 70 75 80Gly Cys Ala Ile Glu Ser Phe Tyr Ile Leu Leu Tyr Phe Phe Tyr Ala 85 90 95Pro Met Gln Ala Lys Lys Gln Thr Leu Lys Val Val Ile Ser Leu Asn 100 105 110Val Gly Val Phe Ser Ile Leu Val Val Leu Ile Gln Phe Leu Leu Lys 115 120 125Gly Ser Asn Arg Ile Asn Val Phe Gly Trp Ile Cys Ala Ser Phe Ser 130 135 140Val Ala Val Phe Ala Ala Pro Leu Ser Ile Val Ala Lys Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr 165 170 175Leu Ser Ala Ile Met Trp Phe Ala Tyr Gly Leu Leu Lys Asn Asp Pro 180 185 190Cys Val Ala Ile Pro Asn Ile Leu Gly Val Ile Leu Gly Leu Val Gln 195 200 205Met Val Leu Tyr Gly Phe Tyr Arg Asn Ala Gly Lys Glu Lys Met Glu 210 215 220Lys Lys Leu Pro Glu His Ile Ile Asp Met Val Met Leu Ser Thr Leu225 230 235 240Gly Thr Ser Asp Ile His Pro Ile Gly Ala Gln Gln Asn Gly Ile Lys 245 250 255Lys Ser Gly Ser Glu Asp Val Lys Asp Asp Glu Glu Thr Gly Asn Arg 260 265 270Glu Lys Ser Thr Glu Asn Ser Gly Glu Leu Gln Pro Asn Gly Ser Thr 275 280 285Val78278PRTVitis vinifera 78Met Ala Val Val Thr Val Lys Gln Leu Ala Phe Ile Phe Gly Leu Leu1 5 10 15Gly Asn Leu Val Ser Phe Met Val Tyr Leu Ser Pro Val Pro Thr Phe 20 25 30Phe Lys Ile Tyr Lys Arg Lys Thr Ser Glu Gly Tyr Gln Ala Leu Pro 35 40 45Tyr Ser Val Gly Leu Leu Cys Ala Ser Leu Phe Leu Tyr Tyr Ala Leu 50 55 60Leu Gln Ser Gly Lys Phe Leu Ile Leu Ser Ile Asn Thr Ile Gly Ser65 70 75 80Thr Ile Gln Ala Thr Tyr Leu Val Leu Phe Ile Ile Tyr Ser Pro Arg 85 90 95Ala Gly Lys Val Ala Thr Leu Lys Met Ile Leu Ile Leu Asn Val Ala 100 105 110Ser Leu Gly Leu Val Leu Leu Leu Thr Thr Leu Phe Ser Lys Gly Lys 115 120 125Thr Arg Ile Gln Val Val Gly Trp Ile Ser Ala Gly Val Asn Ile Gly 130 135 140Thr Phe Val Ala Pro Leu Ser Ile Ile Lys Arg Val Ile Glu Thr Arg145 150 155 160Ser Val Glu Tyr Met Pro Phe Asn Leu Ser Phe Phe Leu Thr Ile Cys 165 170 175Ala Thr Met Trp Phe Phe Tyr Gly Ile Phe Val Arg Asp Phe Phe Ile 180 185 190Ala Ile Pro Asn Val Val Gly Phe Val Phe Gly Ile Ala Gln Met Phe 195 200 205Leu Tyr Ile Ile Tyr Lys Tyr Met Met Lys Ser Asp Glu Thr Thr Leu 210 215 220Glu Gln Leu Glu Glu Thr Thr Glu Arg Pro Leu Tyr Val Pro Thr Ala225 230 235 240Asn His Glu Pro Ser Gly Gln Glu Leu Lys Ala Val Thr Ile Thr Ser 245 250 255Pro Arg Gln Val Asp Tyr Phe Thr Glu His His Pro Met Phe Met Glu 260 265 270Arg Asp Glu Tyr Leu Ser 27579270PRTVitis vinifera 79Met Ala Leu Phe Pro Ile His His Pro Leu Val Phe Ile Phe Gly Ile1 5 10 15Leu Gly Asn Leu Ile Ser Phe Met Val Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Gln Ile Tyr Lys Arg Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Phe Leu Asn Thr Asp Ala Ser Leu Leu Ile Thr Ile Asn Ser Val Gly65 70 75 80Cys Val Ile Glu Thr Ser Tyr Ile Val Met Phe Leu Val Tyr Ala Pro 85 90 95Lys Lys Ala Arg Ile Thr Thr Val Lys Leu Val Phe Leu Met Asn Ile 100 105 110Cys Gly Phe Gly Ser Ile Leu Leu Leu Thr Leu Leu Leu Ala Glu Gly 115 120 125Ala Asn Arg Val Arg Ile Leu Gly Trp Val Cys Leu Val Phe Ser Leu 130 135 140Ser Val Phe Leu Ala Pro Leu

Cys Ile Met Arg Gln Val Ile Arg Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Leu Leu Ser Phe Phe Leu Thr Leu 165 170 175Ser Ala Val Met Trp Phe Phe Tyr Gly Leu Met Leu Lys Asp Phe Tyr 180 185 190Ile Ala Gly Pro Asn Ile Leu Gly Phe Val Phe Gly Ile Val Gln Met 195 200 205Val Leu Tyr Leu Ile Tyr Arg Asn Arg Lys Lys Val Leu Glu Asn Glu 210 215 220Lys Leu Pro Glu Leu Ser Glu Gln Ile Ile Asp Val Val Lys Leu Ser225 230 235 240Thr Met Val Cys Ser Glu Val Asn Leu Thr Asn Gln Gln His Ser Asn 245 250 255Glu Gly His Gly Thr Thr Gly Leu Glu Val Ile Val Ala Leu 260 265 27080217PRTPopulus trichocarpa 80Met Thr Gly Asn Ile Ile Ser Phe Met Val Tyr Leu Ala Pro Val Pro1 5 10 15Thr Phe Ile Arg Ile Leu Arg Lys Lys Ser Thr Glu Asp Phe Gln Ser 20 25 30Leu Pro Tyr Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr 35 40 45Ala Met Leu Lys Asn Asp Glu Ile Leu Leu Val Thr Ile Asn Ser Phe 50 55 60Gly Cys Val Ile Glu Thr Ile Tyr Ile Ala Ile Tyr Ile Ala Tyr Ala65 70 75 80Thr Arg Glu Ser Lys Val Ser Thr Ile Lys Leu Leu Leu Ser Met Asn 85 90 95Met Gly Leu Phe Ser Leu Ile Ile Leu Leu Thr His Phe Leu Ala Ser 100 105 110Gly Ser Thr Arg Val Lys Ala Leu Gly Trp Leu Cys Val Ala Phe Ser 115 120 125Val Cys Val Phe Ala Ala Pro Leu Asn Ile Val Lys Gln Ile Ile Arg 130 135 140Thr Lys Ser Val Glu Phe Met Pro Phe Thr Leu Ser Phe Phe Leu Thr145 150 155 160Leu Ser Ala Val Ile Trp Phe Ala Tyr Gly Leu Phe Ile Lys Asp Met 165 170 175Cys Val Ala Leu Pro Asn Ile Leu Gly Phe Val Leu Gly Leu Leu Gln 180 185 190Met Leu Leu Tyr Gly Ile Tyr Arg Asn Ala Glu Lys Lys Lys Ile Pro 195 200 205Ala Glu Asn Leu Lys Ser Ile Val Ile 210 21581269PRTPopulus trichocarpa 81Met Ala Leu His Phe Thr Trp Val Phe Gly Phe Gly Leu Leu Gly Asn1 5 10 15Ile Ile Ser Cys Leu Val Cys Leu Ala Pro Leu Pro Thr Phe Tyr Gln 20 25 30Ile Cys Lys Lys Lys Thr Ser Gln Gly Phe Gln Ser Ile Pro Tyr Val 35 40 45Ile Ala Leu Phe Ser Ala Met Leu Trp Leu Phe Tyr Ala Ser Phe Ser 50 55 60Glu Asn Ala Met Leu Leu Ile Thr Ile Asn Ser Phe Ala Phe Phe Met65 70 75 80Glu Ile Gly Tyr Ile Ala Val Tyr Leu Phe Tyr Ala Thr Lys Lys Asp 85 90 95Lys Ile Leu Thr Phe Lys Leu Leu Leu Leu Phe Asn Ile Phe Gly Phe 100 105 110Gly Leu Ile Cys Ala Leu Ser Leu Leu Leu Thr Glu Gly Thr Lys Arg 115 120 125Val His Val Leu Gly Trp Ile Cys Met Val Phe Ala Leu Cys Val Phe 130 135 140Val Ala Pro Leu Gly Val Val Arg Lys Val Ile Arg Thr Lys Ser Val145 150 155 160Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Tyr Leu Lys Lys Asp Lys Phe Val Ala Ile 180 185 190Pro Asn Ile Leu Gly Phe Ile Phe Gly Ile Leu Gln Met Val Leu Tyr 195 200 205Leu Ile Tyr Arg Asn Pro Lys Lys Asn Glu Val Ala Glu Pro Arg Thr 210 215 220Gln Glu Leu Ser Glu Gln Tyr Cys Ser Asp Ile Asn Ile Ala Met Pro225 230 235 240Lys Leu Asn Glu Gly Gly Asn Glu Val Phe Glu Ala His Ser Ala Lys 245 250 255Asp Gln Thr Lys Glu Ala Met Asp Val Thr Asn Lys Val 260 26582283PRTPopulus trichocarpa 82Met Ala Leu His Leu Thr Trp Met Leu Ala Phe Gly Leu Leu Gly Asn1 5 10 15Leu Ile Ser Cys Leu Val Cys Leu Ala Pro Leu Pro Thr Phe Tyr Gln 20 25 30Ile Tyr Lys Lys Lys Thr Ser Glu Gly Phe Gln Ser Ile Pro Tyr Val 35 40 45Ile Ala Leu Phe Ser Ala Met Leu Trp Leu Phe Tyr Ala Ile Phe Ser 50 55 60Glu Asp Ala Ile Leu Leu Ile Thr Ile Asn Thr Phe Ala Phe Phe Met65 70 75 80Glu Phe Gly Tyr Ile Thr Val Tyr Leu Leu Tyr Ala Thr Lys Lys Asp 85 90 95Lys Ile Leu Thr Phe Lys Leu Leu Leu Leu Phe Asn Ser Phe Gly Phe 100 105 110Gly Leu Ile Cys Val Leu Thr Leu Phe Leu Thr Gln Gly Gln Lys Arg 115 120 125Val Gln Val Leu Gly Trp Ile Cys Met Ile Phe Ser Leu Cys Val Phe 130 135 140Val Ala Pro Leu Phe Ile Val Arg Glu Val Ile Lys Thr Lys Ser Val145 150 155 160Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Tyr Leu Lys Lys Asp Gln Phe Val Ala Val 180 185 190Pro Asn Ile Leu Gly Phe Leu Phe Gly Ile Ile Gln Met Val Leu Tyr 195 200 205Val Ile Tyr Arg Asn Pro Met Lys Ile Leu Val Val Glu Pro Lys Leu 210 215 220Gln Glu Leu Ser His Glu His Ile Val Asp Ile Arg Lys Leu Gly Thr225 230 235 240Ala Ile Cys Ser Glu Ile Asn Ile Val Ile Pro Gln Leu Asn Asp Ser 245 250 255Gly Lys Val Val Phe Glu Asp Gln Ile Ala Lys Glu Leu Thr Lys Gln 260 265 270Thr Gln Glu Ile Thr Asn Ala Thr Asn Lys Ile 275 28083283PRTPopulus trichocarpa 83Met Ala Leu His Leu Thr Trp Val Phe Gly Phe Gly Leu Leu Gly Asn1 5 10 15Phe Ile Ser Cys Leu Val Cys Leu Ala Pro Leu Pro Thr Phe Tyr Arg 20 25 30Ile Cys Lys Lys Lys Thr Ser Gln Gly Phe His Ser Ile Pro Tyr Val 35 40 45Ile Ala Leu Phe Ser Ala Met Leu Trp Leu Phe Tyr Ala Leu Phe Lys 50 55 60Glu Asp Ala Leu Leu Leu Ile Thr Ile Asn Ser Phe Thr Phe Phe Met65 70 75 80Glu Ile Gly Tyr Ile Phe Met Tyr Leu Leu Tyr Ala Thr Lys Lys Asp 85 90 95Lys Ile Leu Thr Phe Lys Leu Leu Leu Phe Phe Asn Val Phe Gly Phe 100 105 110Gly Leu Ile Cys Val Leu Thr Arg Phe Leu Thr Gln Arg Gln Lys Arg 115 120 125Val Gln Val Leu Gly Trp Ile Cys Met Thr Phe Ser Leu Cys Val Phe 130 135 140Val Ala Pro Leu Phe Ile Val Arg Lys Val Ile Arg Thr Lys Ser Val145 150 155 160Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Phe Leu Lys Lys Asp Gln Phe Val Ala Val 180 185 190Pro Asn Ile Leu Gly Leu Leu Phe Gly Ile Leu Gln Met Val Leu Tyr 195 200 205Met Ile Tyr Gly Asn Ser Lys Lys Val Val Val Leu Glu Pro Lys Leu 210 215 220Lys Leu Asp Ile Ser Glu His Val Val Asp Leu Glu Lys Leu Gly Ala225 230 235 240Ala Ile Cys Ser Glu Ile Ala Ile Gly Ile Pro Lys Leu Asn Asp Gly 245 250 255Gly Asp Gly Ile Ile Glu Asp Gln Asn Ala Lys Glu Gln Thr Lys Lys 260 265 270Ile Met Lys Ala Met Asp Val Thr Asn Lys Leu 275 28084259PRTPopulus trichocarpa 84Met Gly Phe Leu Ser Asn Asp Gln Leu Thr Phe Leu Phe Gly Leu Leu1 5 10 15Gly Asn Ile Val Ala Ala Gly Met Phe Leu Ala Pro Val Pro Thr Phe 20 25 30Tyr Thr Ile Phe Lys Arg Lys Ser Ser Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Ser Val Ala Leu Met Ser Ala Ser Leu Leu Leu Tyr Tyr Gly Leu 50 55 60Leu Lys Thr Asn Ala Tyr Leu Leu Ile Ser Ile Asn Ser Ile Gly Cys65 70 75 80Ala Phe Glu Val Thr Tyr Leu Ile Ile Tyr Leu Ile Tyr Ala Pro Lys 85 90 95Gln Glu Lys Met His Thr Met Lys Leu Leu Leu Ile Phe Asn Met Gly 100 105 110Ser Phe Gly Val Val Leu Leu Leu Thr Met Leu Leu Met Lys Gly Lys 115 120 125Pro Arg Leu Ser Val Val Gly Trp Ile Cys Ala Val Phe Ser Val Ala 130 135 140Val Cys Ala Ala Pro Leu Ser Ile Met Arg Arg Val Val Arg Thr Lys145 150 155 160Ser Val Glu Tyr Leu Pro Phe Thr Leu Ser Ala Ser Ile Thr Leu Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Gln His Asp Tyr Tyr Ile 180 185 190Ala Leu Pro Asn Val Leu Gly Phe Leu Phe Gly Ile Ala Gln Met Ile 195 200 205Leu Tyr Met Val Tyr Lys Asn Leu Lys Lys Asn Val Glu Glu Lys Ser 210 215 220Glu Gln Leu Ala Gly Asn Met Glu Val Val Gln Met Thr Lys Glu Thr225 230 235 240Glu Ser Cys Thr Val Asp Asp Pro His Met Glu Thr Lys Ile Cys Ile 245 250 255Cys Asp Leu85302PRTSorghum bicolor 85Met Ala Gly Leu Ser Leu Gln His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Leu Thr Phe Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Ile Lys Ser Asn Glu Thr Phe Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Val Met Tyr Phe Val Tyr Ala Pro 85 90 95Lys Lys Ala Lys Leu Phe Thr Ala Lys Ile Met Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Val Ile Leu Leu Val Thr Leu Leu Leu Phe Lys Gly 115 120 125Asp Lys Arg Val Val Met Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Met Arg Arg Val Ile Gln Thr145 150 155 160Lys Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Val Val Gln Met 195 200 205Val Leu Tyr Val Leu Tyr Met Asn Lys Thr Pro Val Ala Val Ala Glu 210 215 220Gly Lys Asp Ala Gly Val Lys Leu Pro Ser Ala Ala Asp Glu His Val225 230 235 240Leu Val Asn Ile Thr Lys Leu Ser Pro Ala Leu Pro Asp Arg Ser Ser 245 250 255Gly Val His Arg Ala Thr Gln Met Ala Ala Val Pro Ala Ser Ser Cys 260 265 270Ala Ala Glu Ala Ala Ala Pro Ala Met Leu Pro Asn Arg Asp Val Val 275 280 285Asp Val Phe Val Ser Arg Gln Ser Pro Ala Val His Val Val 290 295 30086302PRTSorghum bicolor 86Met Ala Gly Leu Ser Leu Gln His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Leu Ile Ser Phe Leu Thr Phe Leu Ala Pro Ile Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Thr Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Phe Tyr Ala 50 55 60Leu Ile Lys Ser Asn Glu Thr Phe Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Val Met Tyr Phe Val Tyr Ala Pro 85 90 95Lys Lys Ala Lys Leu Phe Thr Ala Lys Ile Met Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Val Ile Leu Leu Val Thr Leu Leu Leu Phe Lys Gly 115 120 125Asp Lys Arg Val Val Met Leu Gly Trp Ile Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Met Arg Arg Val Ile Gln Thr145 150 155 160Lys Ser Met Glu Tyr Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Thr Phe Gly Met Val Gln Met 195 200 205Val Leu Tyr Val Leu Tyr Met Asn Lys Thr Pro Val Ala Val Ala Glu 210 215 220Gly Lys Asp Ala Gly Gly Lys Leu Pro Ser Ala Gly Asp Lys His Val225 230 235 240Leu Val Asn Ile Ala Lys Leu Ser Pro Ala Leu Pro Glu Arg Ser Ser 245 250 255Gly Val His Arg Ala Thr Gln Met Ser Ala Val Pro Ala Lys Ser Cys 260 265 270Ala Ala Glu Ala Thr Ala Pro Lys Val Met Leu Pro Asn Arg Asp Val 275 280 285Val Asp Val Phe Leu Ser Gln Ala Leu His Arg Lys Gln Ala 290 295 30087309PRTSorghum bicolor 87Met Ala Gly Gly Leu Phe Ser Met Ala His Pro Ala Ile Thr Leu Ser1 5 10 15Gly Ile Ala Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Val 20 25 30Ala Thr Phe Leu Gln Val Tyr Arg Lys Lys Ser Thr Gly Gly Phe Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Ser Val Leu Trp Ile Phe 50 55 60Tyr Ala Leu Val Lys Thr Asn Ser Arg Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Gly Val Glu Ala Ala Tyr Ile Val Phe Tyr Leu Ala Tyr 85 90 95Ala Pro Arg Lys Ala Arg Leu Arg Thr Leu Ala Tyr Phe Phe Leu Leu 100 105 110Asp Val Ala Ala Phe Ala Leu Val Val Val Val Thr Leu Phe Val Val 115 120 125Arg Glu Pro His Arg Val Lys Phe Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Ile Ile Val Lys Val Val145 150 155 160Lys Thr Lys Ser Val Glu Phe Leu Pro Ile Ser Leu Ser Phe Cys Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Cys Tyr Gly Leu Phe Thr Lys Asp 180 185 190Pro Phe Val Met Tyr Pro Asn Val Gly Gly Phe Phe Phe Ser Cys Val 195 200 205Gln Met Gly Leu Tyr Phe Trp Tyr Arg Lys Pro Arg Pro Ala Lys Asn 210 215 220Asn Ala Val Leu Pro Thr Thr Thr Asp Gly Ala Ser Ala Val Gln Met225 230 235 240Gln Gly Gln Val Ile Glu Leu Ala Pro Asn Thr Val Ala Ile Leu Ser 245 250 255Val Ser Pro Ile Pro Ile Val Gly Val His Lys Ile Glu Val Val Glu 260 265 270Gln Gln His Lys Glu Ala Ala Val Ala Ala Glu Thr Arg Arg Met Ala 275 280 285Ala Ala Asn Pro Asp Gly Ala Met Pro Glu Val Ile Glu Ile Val Pro 290 295 300Ala Val Ala Thr Val30588291PRTSorghum bicolor 88Met Ala Gly Leu Ser Leu Gln His Pro Met Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Tyr Arg 20 25 30Pro Thr Phe Tyr Arg Ile Tyr Lys Ser Lys Ser Thr Gln Gly Phe Gln 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr 50 55 60Tyr Ala Leu Leu Lys Ser Asn Glu Phe Leu Leu Ile Thr Ile Asn Ser65 70 75 80Ala Gly Cys Val Ile Glu Thr Leu Tyr Ile Val Met Tyr Leu Leu Tyr 85 90 95Ala Pro Lys Lys Ala Lys Leu Phe Thr Ala Lys

Ile Leu Leu Leu Leu 100 105 110Asn Val Gly Val Phe Gly Leu Ile Leu Leu Leu Thr Leu Leu Leu Ser 115 120 125Ala Gly Gln His Arg Val Val Val Leu Gly Trp Val Cys Val Ala Phe 130 135 140Ser Val Ser Val Phe Val Ala Pro Leu Ser Ile Ile Arg Gln Val Val145 150 155 160Arg Thr Arg Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Ser Leu 165 170 175Thr Val Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp 180 185 190Lys Tyr Val Ala Leu Pro Asn Val Leu Gly Phe Ser Phe Gly Val Val 195 200 205Gln Met Gly Leu Tyr Ala Leu Tyr Arg Asn Ala Thr Pro Arg Val Pro 210 215 220Pro Ala Lys Glu Val Thr Asp Asp Asp Ala Ala Ala Asp Gly Thr Phe225 230 235 240Lys Leu Pro Gly Glu His Val Val Thr Ile Ala Lys Leu Thr Ala Val 245 250 255Pro Ala Val Ser Pro Gln Leu Gln Glu Glu Ala Lys Pro Ala Asp Asn 260 265 270Gly Thr Thr Pro Ala Pro Ala Pro Ala Asn Asp Val Gln Leu Asn Ala 275 280 285Glu Gln Val 29089336PRTSorghum bicolor 89Met Ala Phe Leu Asn Met Glu Gln Gln Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Ile Ile Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Thr Pro Tyr Val Val Thr Leu Phe Ser Cys Met Leu Trp Ile Phe Tyr 50 55 60Ala Leu Leu Lys Ser Gly Ala Glu Leu Leu Val Thr Ile Asn Gly Val65 70 75 80Gly Cys Val Ile Glu Thr Val Tyr Leu Gly Met Tyr Leu Leu Tyr Ala 85 90 95Pro Lys Ala Ala Arg Val Leu Thr Ala Lys Met Leu Leu Gly Leu Asn 100 105 110Val Gly Val Phe Gly Leu Val Ala Leu Val Thr Met Val Leu Ser Asn 115 120 125Gly Gly Leu Arg Val Lys Val Leu Gly Trp Ile Cys Val Ser Val Ala 130 135 140Leu Ser Val Phe Ala Ala Pro Leu Ser Ile Met Arg Gln Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Ile Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Ile Trp Phe Ala Tyr Gly Ala Leu Lys Lys Asp Val 180 185 190Phe Val Ala Ala Pro Asn Val Leu Gly Phe Val Phe Gly Leu Ala Gln 195 200 205Met Ala Leu Tyr Met Ala Tyr Arg Asn Lys Lys Pro Ala Ala Ala Ala 210 215 220Val Ile Met Val Glu Glu Val Lys Leu Pro Ala Glu Gln Tyr Ala Ser225 230 235 240Lys Glu Val Ala Pro Pro Ala Ala Ala His Glu Gly Ser Arg Ala Ser 245 250 255Cys Gly Ala Glu Val His Pro Ile Asp Ile Asp Thr Leu Pro Val Ala 260 265 270Asp Val Gly Arg His His Asp Ser Gln Ala Val Val Val Ile Asp Val 275 280 285Asp Val Glu Pro Ala Ala Thr Cys Ala Ala Ala Ala Ala Ala Ala Gly 290 295 300Gly Val Arg Gly Asp Gly Ala Ala Gly Val Val Thr Ala Gly Pro Glu305 310 315 320Gln Pro Ala Ala Met Lys Pro Val Asp Met Ala Ile Ala Val Glu Ala 325 330 33590273PRTSorghum bicolor 90Met Ala Gly Gly Leu Phe Ser Met Glu His Pro Trp Val Ser Ala Phe1 5 10 15Gly Ile Leu Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Val 20 25 30Pro Thr Phe Leu Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Cys Thr Leu Trp Ile Leu 50 55 60Tyr Ala Val Val Lys Thr Asn Ser Ser Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Val Val Glu Ala Thr Tyr Ile Leu Leu Tyr Leu Ile Tyr 85 90 95Ala Pro Arg Ala Ala Arg Leu Arg Ala Leu Ala Phe Phe Phe Leu Leu 100 105 110Asp Val Ala Ala Leu Ala Leu Ile Val Val Val Val Val Val Leu Val 115 120 125Ala Glu Pro His Arg Val Lys Val Leu Gly Ser Ile Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Val Ile Phe Val Val Ile145 150 155 160Arg Thr Lys Ser Ala Glu Phe Met Pro Phe Thr Leu Ser Phe Phe Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Leu Tyr Gly Ile Phe Thr Lys Asp 180 185 190Pro Tyr Val Thr Leu Pro Asn Val Gly Gly Phe Phe Phe Gly Cys Ile 195 200 205Gln Met Val Leu Tyr Cys Cys Tyr Arg Lys Pro Ser Ala Ser Val Val 210 215 220Leu Pro Thr Thr Thr Asp Ala Ala Ala Thr Glu Met Glu Leu Pro Leu225 230 235 240Ala Ala His Gln Ala Val Ala Pro Val Leu Ala Glu Leu Gln Lys Leu 245 250 255Glu Glu Ala Met Gly Ser Pro Arg Lys His Gly Gly Val Val Lys Val 260 265 270Val91313PRTSorghum bicolor 91Met Ile Thr Val Gly His Pro Val Val Phe Ala Val Gly Ile Leu Gly1 5 10 15Asn Ile Leu Ser Phe Leu Val Thr Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Tyr Lys Lys Lys Ser Thr Glu Ser Phe Gln Ser Val Pro Tyr 35 40 45Val Val Ala Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Ser Ile Asp Val Leu Leu Leu Ser Ile Asn Thr Ile Ala Cys Val Val65 70 75 80Glu Ser Val Tyr Leu Ala Ile Tyr Leu Thr Tyr Ala Pro Lys Pro Ala 85 90 95Met Ala Phe Thr Leu Lys Leu Leu Phe Thr Met Asn Met Gly Leu Phe 100 105 110Gly Ala Met Val Ala Phe Leu Gln Phe Tyr Val Asp Gly Gln Arg Arg 115 120 125Val Ser Ile Ala Gly Gly Val Gly Ala Ala Phe Ala Leu Ala Val Phe 130 135 140Val Ala Pro Leu Thr Ile Ile Arg Gln Val Ile Arg Thr Lys Ser Val145 150 155 160Glu Tyr Met Pro Phe Trp Leu Ser Phe Phe Leu Thr Ile Ser Ala Val 165 170 175Val Trp Phe Phe Tyr Gly Leu Leu Met Lys Asp Phe Phe Val Ala Met 180 185 190Pro Asn Val Leu Gly Leu Leu Phe Gly Leu Ala Gln Met Ala Leu Tyr 195 200 205Phe Val Tyr Arg Asn Arg Asn Pro Lys Gln Asn Gly Ala Val Ser Glu 210 215 220Met Gln Gln Gln Ala Ala Val Val Gln Ala Asp Ala Asp Ala Lys Lys225 230 235 240Glu Gln Gln Leu Arg Gln Ala His Ala Asp Ala Gly Ala Asp Gly Glu 245 250 255Ala Val Ala Val Arg Ile Asp Asp Glu Glu Glu Pro Lys Asn Val Val 260 265 270Val Asp Ile Met Pro Pro Pro Pro Pro Leu Leu Pro Ala Glu Arg Ala 275 280 285Ser Pro Pro Leu Pro Leu Pro Pro Pro Pro Ala Met Val Met Met Thr 290 295 300Ala His Gln Thr Ala Val Glu Val Val305 31092297PRTRicinus communis 92Met Ala Ile Phe Asn Thr His Asn Pro Ser Val Phe Val Phe Gly Leu1 5 10 15Leu Gly Asn Ile Val Ser Phe Val Val Phe Leu Ala Pro Val Pro Thr 20 25 30Phe Leu Arg Val Cys Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Phe 35 40 45Pro Tyr Val Val Ser Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr Ala 50 55 60Ser Leu Lys Ser Asp Ala Phe Leu Leu Ile Thr Ile Asn Ser Val Gly65 70 75 80Cys Leu Ile Glu Thr Ile Tyr Ile Thr Leu Phe Ile Thr Tyr Ala Pro 85 90 95Lys Gln Ala Arg Ile Thr Thr Leu Lys Ile Leu Leu Leu Leu Asn Phe 100 105 110Gly Gly Phe Cys Leu Ile Leu Leu Leu Ser His Phe Leu Ala Lys Gly 115 120 125Ser Glu Arg Ala Thr Ile Leu Gly Trp Val Cys Val Ile Phe Ser Val 130 135 140Ser Val Phe Ala Ala Pro Leu Ser Val Met Arg Ile Val Ile Arg Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Tyr Leu Ser Phe Phe Leu Thr Leu 165 170 175Ser Ala Ile Met Trp Leu Phe Tyr Gly Leu Leu Leu Lys Asp Leu Tyr 180 185 190Ile Ala Val Pro Asn Ile Leu Gly Leu Val Phe Gly Val Leu Gln Met 195 200 205Ile Leu Tyr Val Ile Tyr Lys Asn Val Lys Thr Val Val Glu Glu Pro 210 215 220Lys Leu Pro Glu His Asn Val Asp Asn Val Lys Leu Ser Ala Val Ile225 230 235 240Thr Cys Glu Val Gln Gln Glu Val Cys Ser Gln Ser Gln Pro Asn Gly 245 250 255Asp Asp Gly Ala His Asn Lys Glu Gln Lys Met His Asp Asn Pro Ala 260 265 270Asn Ala Val Thr Glu Tyr Gln Arg His Ser Gly Met Asp Ala Ser Ile 275 280 285Ala Asp Gln Ser Ile Ala Cys Arg Val 290 29593277PRTRicinus communis 93Met Ala Tyr His Leu Ser Leu Glu Phe Leu Phe Gly Val Leu Ala Asn1 5 10 15Ile Ile Ser Ser Met Val Cys Leu Ala Pro Leu Pro Thr Phe Tyr Gln 20 25 30Ile Cys Lys Lys Lys Thr Ser Glu Gly Phe Gln Ser Val Pro Tyr Val 35 40 45Ile Ala Leu Phe Ser Ala Met Leu Trp Leu Phe Tyr Ala Thr Phe Asp 50 55 60Asp Asn Ala Thr Leu Leu Ile Thr Ile Asn Ser Phe Thr Phe Phe Met65 70 75 80Glu Val Gly Tyr Leu Ser Val Tyr Leu Phe Tyr Gly Thr Arg Lys Asp 85 90 95Arg Met Leu Thr Thr Lys Leu Val Leu Phe Phe Asn Val Phe Gly Phe 100 105 110Gly Met Ile Ala Ile Leu Thr Leu Phe Leu Thr His Gly Arg Lys Arg 115 120 125Val Asp Val Leu Gly Trp Ile Cys Met Ile Phe Ala Leu Cys Val Phe 130 135 140Val Ala Pro Leu Gly Ile Met Arg Lys Val Ile Lys Thr Lys Ser Val145 150 155 160Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Phe Leu Lys Lys Asp Ile Tyr Val Tyr Ile 180 185 190Pro Asn Val Leu Gly Phe Phe Phe Gly Ile Val Gln Met Ile Leu Tyr 195 200 205Leu Ile Tyr Arg Asn Ser Lys Lys Pro Val Glu Glu Pro Lys Ser Gln 210 215 220Glu Phe Ser Glu His Ile Val Asp Val Ala Lys Leu Ser Ala Val Ile225 230 235 240Cys Ser Glu Leu Lys Thr Met Val Val Ala Lys Leu Asn Asp Asn Gly 245 250 255Asn Glu Val Val Lys Glu Glu Thr Lys Asn Thr Lys Gln Glu Met Glu 260 265 270Ala Ser Asn Lys Val 27594279PRTRicinus communis 94Met Ala Phe His Leu Thr Leu Ala Phe Ala Phe Gly Leu Leu Gly Asn1 5 10 15Ile Ile Ser Phe Leu Val Cys Leu Ala Pro Met Pro Thr Phe Tyr Gln 20 25 30Ile Cys Lys Lys Lys Thr Ser Glu Gly Phe Gln Ser Ile Pro Tyr Val 35 40 45Ile Ala Leu Phe Ser Ala Thr Leu Trp Leu Phe Tyr Ala Ile Phe Ala 50 55 60Asn Asp Ala Thr Leu Leu Ile Thr Ile Asn Ser Phe Ala Phe Phe Met65 70 75 80Glu Thr Ala Tyr Ile Ala Ile Tyr Leu Phe Tyr Ala Val Lys Lys Asp 85 90 95Arg Leu Phe Thr Thr Lys Leu Val Leu Ser Leu Asn Ile Phe Ala Phe 100 105 110Gly Ser Ile Cys Val Ile Ala Met Phe Leu Thr His Gly Gln Lys Arg 115 120 125Val Gln Leu Leu Gly Trp Ile Cys Met Val Phe Ala Leu Cys Val Phe 130 135 140Val Ala Pro Leu Ala Ile Val Arg Lys Val Ile Lys Thr Lys Ser Val145 150 155 160Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Phe Leu Lys Lys Asp Leu Tyr Val Ala Val 180 185 190Pro Asn Ile Leu Gly Phe Met Phe Gly Val Leu Gln Met Ile Leu Tyr 195 200 205Leu Ile Tyr Arg Asn Pro Lys Lys Thr Gly Asp Asp Asp Gln Lys Ala 210 215 220Asn Glu Leu Pro Asn Gln His Ser Ile Ile Asp Val Ala Lys Leu Asn225 230 235 240Thr Arg Val Ser Cys Cys Glu Pro Asn Ala Thr Thr Val Ala His Ser 245 250 255Arg Asn Asp Arg Glu Glu Gln Gln Thr Met Gln Ile Asn Arg Glu Asp 260 265 270Lys Asp Ala Thr Asn Thr Val 27595272PRTRicinus communis 95Met Ala Ile Ile Ser Thr His Pro Pro Leu Ala Phe Ala Phe Gly Ile1 5 10 15Leu Gly Asn Ile Ile Ser Ile Leu Val Tyr Leu Ala Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu 35 40 45Pro Tyr Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala 50 55 60Met Leu Lys Lys Asp Val Phe Leu Leu Val Thr Ile Asn Ala Phe Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Ile Met Tyr Ile Ile Tyr Ala Thr 85 90 95Lys Lys Asn Arg Val Ser Thr Phe Lys Val Leu Thr Ser Met Asn Leu 100 105 110Gly Leu Phe Ala Phe Ile Ile Leu Phe Ser His Phe Leu Val Lys Ser 115 120 125Ser Val Arg Ala Gln Val Leu Gly Trp Ile Cys Val Ala Val Ser Val 130 135 140Cys Val Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Ile Lys Thr145 150 155 160Arg Ser Val Glu Phe Met Pro Phe Asn Leu Ser Phe Phe Leu Thr Leu 165 170 175Ser Ala Ile Met Trp Phe Ala Tyr Gly Leu Ser Thr Lys Asp Thr Cys 180 185 190Val Ala Leu Pro Asn Val Leu Gly Phe Ile Leu Gly Leu Leu Gln Met 195 200 205Val Leu Tyr Val Ile Tyr Arg Lys Ala Lys Lys Val Ile Leu Glu Glu 210 215 220Lys Leu Pro Glu His Leu Lys Thr Ile Val Val Leu Ser Thr Leu Gly225 230 235 240Asn Ser Glu Gln Gln Leu Val Thr Pro Val Gly Ser Leu Leu Ile Phe 245 250 255Gly Gln Phe Arg Asp Val Ala Thr Cys Arg Arg Phe Gln Ile Leu Leu 260 265 27096286PRTRicinus communis 96Met Ala Leu Asn Asp Pro Arg Phe Ile Leu Ala Phe Gly Ile Leu Gly1 5 10 15Asn Ile Val Ser Phe Leu Val Tyr Leu Ala Pro Leu Pro Thr Phe Trp 20 25 30Arg Ile Val Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Ile Pro Tyr 35 40 45Ser Val Ala Leu Phe Ser Ala Met Leu Thr Leu Tyr Tyr Ala Thr Leu 50 55 60Lys Glu Asn Ala Ile Leu Leu Ile Thr Ile Asn Ser Ile Gly Cys Leu65 70 75 80Ile Glu Gly Ile Tyr Leu Thr Ile Tyr Met Ile Tyr Ala Thr Gln Thr 85 90 95Ser Arg Val Gln Ile His Phe Lys Leu Leu Ile Leu Phe Asn Leu Gly 100 105 110Thr Tyr Leu Leu Ile Val Met Leu Ala Ser Glu Leu Thr His Gly Thr 115 120 125Leu Arg Val Gln Val Val Gly Trp Ile Cys Ala Val Phe Ser Val Cys 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Leu Val Ile Lys Thr Lys145 150 155 160Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Cys 165 170 175Ala Ile Ser Trp Leu Gly Tyr Gly Leu Ala Val Asn Asp Tyr Phe Ile 180 185 190Ala Ser Pro Asn Ile Leu Gly Phe Leu Phe Gly Ile Val Gln Met Val 195 200 205Leu Tyr Met Ile Tyr Lys Asn Lys Lys Asn Glu Ile Leu Pro Thr Ser 210 215

220Thr Ser Gln Glu Leu Ala Val Ser Lys Pro Glu Thr Ser Gln Asp Arg225 230 235 240Glu Asn Ser Asn Ser Ser Ser Leu Asn Gln Gln Asp Leu Glu Ala Ala 245 250 255Lys Asp Asp Arg Arg Glu Asn Asn Lys Ala Val Pro Glu Glu Ala Ser 260 265 270Glu Arg Asn Gly Tyr Arg Ala Thr Cys Phe Gly Ile Ser Phe 275 280 28597294PRTArabiopsis lyrata 97Met Ala Leu Thr His Asn Val Trp Ala Phe Val Phe Gly Met Leu Gly1 5 10 15Asn Ile Ile Ser Phe Val Val Phe Leu Ala Pro Val Pro Thr Phe Val 20 25 30Arg Ile Cys Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Val Ser Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Met Gln 50 55 60Lys Asp Gly Ser Gly Phe Leu Leu Ile Thr Ile Asn Ala Val Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Ile Val Leu Phe Val Thr Tyr Ala Asn Lys 85 90 95Lys Thr Arg Ile Ser Thr Leu Lys Val Leu Gly Leu Leu Asn Phe Leu 100 105 110Gly Phe Ala Ala Ile Val Leu Val Cys Glu Leu Leu Thr Glu Gly Ser 115 120 125Thr Arg Glu Lys Val Leu Gly Gly Ile Cys Val Gly Phe Ser Val Ser 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Val Val Val Arg Thr Arg145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Ile Ser 165 170 175Ala Val Thr Trp Leu Phe Tyr Gly Leu Ala Ile Lys Asp Phe Tyr Val 180 185 190Ala Leu Pro Asn Val Leu Gly Ala Phe Leu Gly Ala Val Gln Met Ile 195 200 205Leu Tyr Ile Ile Phe Lys Tyr Tyr Lys Ile Pro Met Ala Gln Lys Thr 210 215 220Asp Lys Ser Lys Ala Val Ser Asp His Ser Ile Asp Ile Ala Lys Leu225 230 235 240Thr Thr Val Thr Pro Gly Pro Ile Ser Asp Ser Ala Val His Gln Pro 245 250 255Pro Leu Ile His Asn Val Pro Glu Thr Gln Ile Gln Val Thr Glu Val 260 265 270Lys Ser Gln Asn Ile Thr Asp Pro Lys Asp Gln Ile Asn Lys Asp Val 275 280 285Glu Asn Gln Ser Gln Val 29098262PRTGlycine max 98Met Val Ser Ile Ser Asp His Glu Leu Val Leu Ile Phe Gly Leu Leu1 5 10 15Gly Asn Ile Val Ser Phe Met Val Phe Leu Ala Pro Leu Pro Thr Phe 20 25 30Tyr Thr Ile Tyr Lys Lys Lys Ser Ser Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Ala Val Ala Leu Leu Ser Ala Leu Leu Leu Leu Tyr Tyr Gly Phe 50 55 60Ile Lys Thr Asn Ala Thr Leu Ile Ile Thr Ile Asn Cys Ile Gly Cys65 70 75 80Val Ile Glu Val Ser Tyr Leu Thr Met Tyr Ile Ile Tyr Ala Pro Arg 85 90 95Lys Gln Lys Ile Ser Thr Leu Val Met Ile Leu Ile Ala Asp Ile Gly 100 105 110Gly Phe Gly Leu Thr Met Leu Ile Thr Thr Phe Ala Val Lys Gly Ile 115 120 125Asn Arg Val His Ala Val Gly Trp Ile Cys Ala Ile Phe Asn Ile Ala 130 135 140Val Phe Ala Ala Pro Leu Ser Ile Met Arg Arg Val Ile Lys Thr Lys145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Leu Cys 165 170 175Ala Thr Met Trp Phe Phe Tyr Gly Phe Phe Asp Lys Asp Asp Phe Ile 180 185 190Met Phe Pro Asn Val Leu Gly Phe Ile Phe Gly Ile Ser Gln Met Ile 195 200 205Leu Tyr Met Ile Tyr Lys Asn Ser Lys Lys Asn Gly Glu Thr Asn Cys 210 215 220Thr Glu Gln Gln Glu Ser Glu Gly Thr Val Asn Ser Lys Gln His Ser225 230 235 240Cys Asp Gly Asn Lys Leu Asp Phe Pro Ser Leu Val Glu Met Lys Glu 245 250 255Asn Gln Leu Asn Gln Val 26099258PRTGlycine max 99Met Ala Ile Ser His Glu Thr Trp Ala Phe Ile Phe Gly Leu Leu Gly1 5 10 15Asn Val Ile Ser Phe Met Val Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Gln Ile Tyr Lys Lys Lys Ser Ser Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Leu Val 50 55 60Lys Lys Asp Ala Ser Leu Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys65 70 75 80Val Ile Glu Thr Ile Tyr Leu Ala Ile Phe Leu Val Tyr Ala Pro Ser 85 90 95Lys Thr Arg Leu Trp Thr Ile Lys Leu Leu Leu Met Leu Asn Val Phe 100 105 110Gly Phe Gly Gly Met Leu Leu Ser Thr Leu Tyr Leu Thr Thr Gly Ser 115 120 125Lys Arg Leu Ser Val Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Ser 130 135 140Val Phe Ala Ala Pro Leu Cys Ile Met Lys Arg Val Ile Lys Thr Arg145 150 155 160Ser Val Glu Phe Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Tyr Tyr Ile 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Leu Phe Gly Ile Ile Gln Met Val 195 200 205Leu Tyr Leu Val Tyr Arg Asn Ala Lys Pro Gln Thr Leu Glu Glu Pro 210 215 220Thr Lys Val Gln Glu Leu Asn Gly His Ile Ile Asp Val Val Lys Pro225 230 235 240Asn His Ala Thr Lys Asn Gly His Val Pro Val Ile Glu Ile Ala Ser 245 250 255Ser Val100283PRTGlycine max 100Met Ala Ile Phe Asn Gly His Asn His Leu Ala Leu Gly Phe Gly Met1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Val Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu 35 40 45Pro Tyr Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala 50 55 60Ser Leu Lys Pro Ala Asp Ala Thr Leu Leu Ile Thr Ile Asn Ser Leu65 70 75 80Gly Cys Val Ile Glu Ile Val Tyr Ile Ile Met Phe Thr Ile Tyr Ala 85 90 95Thr Lys Asp Ala Arg Asn Leu Thr Val Lys Leu Phe Met Val Met Asn 100 105 110Val Gly Ser Phe Ala Leu Ile Phe Leu Val Thr Tyr Phe Ala Met His 115 120 125Gly Ser Leu Arg Val Gln Val Val Gly Trp Val Cys Val Ser Ile Ala 130 135 140Val Gly Val Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Ile Arg145 150 155 160Thr Lys Asn Val Glu Phe Met Pro Phe Asn Leu Ser Leu Phe Leu Thr 165 170 175Ile Ser Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Ile 180 185 190Cys Ile Ala Ile Pro Asn Ile Leu Gly Phe Thr Leu Gly Leu Leu Gln 195 200 205Met Leu Leu Tyr Ala Ile Tyr Arg Asn Gly Lys Thr Asn Asn Lys Glu 210 215 220Val Val Thr Lys Glu Glu His Ala Leu Glu Ala Met Lys Asn Val Val225 230 235 240Val Val Asn Pro Leu Gly Thr Cys Glu Val Tyr Pro Val Ile Gly Lys 245 250 255Glu Ile Asn Asn Asn Gly Gln Gly Ile Glu Gly Ala Glu Glu Lys Glu 260 265 270Lys Gly Val Glu Leu Gly Lys Glu Cys Pro Val 275 280101272PRTGlycine max 101Met Val Ile Thr His His Thr Leu Ala Phe Thr Phe Gly Met Leu Gly1 5 10 15Asn Val Ile Ser Phe Leu Val Phe Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Ile Tyr Lys Lys Lys Ser Thr Glu Ser Phe Gln Ser Leu Pro Tyr 35 40 45Leu Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Lys Arg Asp Ala Val Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Ile Glu Ile Ile Tyr Ile Val Leu Tyr Ile Thr Tyr Ala Thr Arg Asp 85 90 95Ala Arg Asn Leu Thr Ile Lys Leu Phe Ser Ala Met Asn Met Thr Ser 100 105 110Phe Ala Val Ile Leu Leu Val Thr His Phe Gly Val His Gly Pro Leu 115 120 125Arg Val Gln Val Leu Gly Trp Ile Cys Val Ser Ile Ser Val Ser Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Val Ala Gln Val Val Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Asn Leu Ser Phe Thr Leu Thr Leu Ser Ala 165 170 175Ile Met Trp Phe Gly Tyr Gly Leu Phe Leu Lys Asp Ile Cys Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Val Leu Gly Leu Leu Gln Met Leu Leu 195 200 205Tyr Thr Ile Tyr Arg Lys Gly Asn Lys Lys Thr Asn Thr Asn Glu Lys 210 215 220Ser Leu Ser Val Lys Pro Leu Lys Asn Ile Ala Val Val Asn Pro Leu225 230 235 240Gly Thr Gly Glu Val Phe Pro Val Glu Glu Asp Glu Gln Ala Ala Lys 245 250 255Lys Ser Gln Gly Asp Gly Glu Asp Lys Lys Ala Glu Asp Cys Leu Val 260 265 270102268PRTGlycine maxmisc_feature(244)..(244)Xaa can be any naturally occurring amino acid 102Met Pro Thr His His Ala Ser Ala Ala Ile Phe Gly Ile Ile Gly Asn1 5 10 15Met Ile Ser Val Met Val Tyr Leu Ala Pro Val Pro Thr Phe Tyr Gln 20 25 30Ile Tyr Lys Lys Lys Cys Thr Asp Gly Phe His Ser Leu Pro Tyr Leu 35 40 45Leu Ser Leu Met Ser Ser Met Leu Trp Leu Tyr Tyr Ala Phe Leu Lys 50 55 60Ile His Asp Gly Val Val Pro Leu Ile Thr Ile Asn Ser Ile Gly Cys65 70 75 80Val Ile Glu Leu Ile Tyr Ile Leu Thr Tyr Ile Lys Tyr Ala His Lys 85 90 95Asp Ala Arg Asn Leu Thr Tyr Thr Leu Phe Ala Ala Met Asn Ile Ala 100 105 110Phe Leu Thr Leu Val Leu Ser Ser His Phe Ala Leu His Gly Ser His 115 120 125Arg Val Lys Val Ile Gly Trp Ile Cys Asp Ala Val Ser Leu Ser Val 130 135 140Phe Ala Ser Pro Leu Ser Ile Met Ala Lys Val Ile Arg Thr Lys Ser145 150 155 160Val Gln Phe Met Pro Phe Tyr Leu Ser Phe Phe Leu Thr Leu Asn Ala 165 170 175Ile Thr Trp Phe Val Tyr Gly Leu Ser Ile Gln Asp Lys Cys Ile Tyr 180 185 190Val Pro Asn Val Gly Gly Phe Gly Leu Gly Leu Val Gln Met Val Leu 195 200 205Tyr Gly Ile Tyr Arg Asn Gly Gly Glu Ser Glu Lys Glu Gln Ala Leu 210 215 220Ala Glu Gly Ala Ile Asn Ile Val Val Val Asn Pro Leu Gly Pro Ala225 230 235 240Glu Val Phe Xaa Ile Ala Glu Glu Val Asp Asp Asp Lys Val Lys Glu 245 250 255Gly Leu Val Val Asp Gln Glu Lys Asp Ala Lys Asp 260 265103298PRTBrachypodium distachyon 103Met Ala Ala Ile Gly Asn Pro Trp Val Phe Ala Val Gly Ile Leu Gly1 5 10 15Asn Ile Leu Ser Phe Leu Val Ile Leu Ala Pro Val Pro Thr Phe His 20 25 30Arg Val Tyr Lys Arg Lys Ser Thr Glu Ser Phe Gln Ser Ala Pro Tyr 35 40 45Ala Met Ala Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Leu Leu 50 55 60Thr Ala Asp Leu Leu Leu Leu Ser Ile Asn Ala Val Gly Cys Val Val65 70 75 80Glu Thr Ala Tyr Leu Ala Val Tyr Leu Ala Tyr Ala Pro Lys Gln Ala 85 90 95Arg Ala Phe Thr Val Lys Leu Val Phe Val Met Asn Val Ala Leu Tyr 100 105 110Gly Ala Met Val Ala Phe Leu Gln Leu Tyr Val Arg Asp Gly Asp Arg 115 120 125Arg Val Ala Ile Ala Gly Gly Val Gly Ala Ala Phe Ala Phe Ala Val 130 135 140Phe Val Ala Pro Leu Ala Ile Ile Arg Gln Val Ile Arg Thr Lys Ser145 150 155 160Val Glu Phe Leu Pro Phe Trp Leu Ser Phe Phe Leu Thr Ile Ser Ala 165 170 175Val Val Trp Phe Phe Tyr Gly Leu Leu Met Lys Asp Phe Phe Val Ala 180 185 190Met Pro Asn Val Leu Gly Leu Leu Phe Gly Leu Ala Gln Met Ala Leu 195 200 205His Leu Val Tyr Lys Asn Pro Lys Lys Lys Lys Gly Ala Val Ser Glu 210 215 220Ala Gly Gln Ala Ala Val Ala Ala Asp Gly Glu Lys Gln Asn Gln Leu225 230 235 240Glu Leu Gln Gln Gln His Gln Gln Ala Ala Ala Ala Thr Ile Asn Ala 245 250 255Asp Asp Ala Glu Asp Ala Ser Lys Val Gln Gln Ser Val Thr Val Val 260 265 270Val Asp Ile Pro Leu Pro Pro Pro Glu Glu His Pro Ala Pro Met Pro 275 280 285Pro Pro Ile Arg Thr Ala Val Glu Val Val 290 295104299PRTBrachypodium distachyon 104Met Ala Ala Gly Phe Leu Ser Met Ala His Pro Ala Ile Thr Leu Ser1 5 10 15Gly Ile Ala Gly Asn Val Ile Ser Phe Leu Val Phe Leu Ala Pro Val 20 25 30Thr Thr Phe Val Gln Val Val Arg Lys Lys Thr Thr Gly Gly Phe Ser 35 40 45Ala Val Pro Tyr Val Val Ala Leu Phe Ser Ser Thr Leu Trp Ile Leu 50 55 60Tyr Ala Leu Leu Lys Gly Asn Ser Arg Pro Leu Leu Thr Ile Asn Gly65 70 75 80Phe Gly Cys Gly Val Glu Leu Ala Tyr Val Val Ala Tyr Leu Leu Tyr 85 90 95Ala Pro Arg Lys Ala Arg Leu Arg Ala Leu Ala Tyr Phe Leu Ala Leu 100 105 110Asp Val Ala Ala Phe Ala Ile Val Ala Ala Val Ala Leu Leu Gly Val 115 120 125Ala Pro Glu His Arg Val Lys Phe Leu Gly Ser Val Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Ile Ile Phe Lys Val Ile145 150 155 160Lys Thr Lys Ser Val Glu Phe Met Pro Ile Ser Leu Ser Phe Cys Leu 165 170 175Val Leu Ser Ala Val Ala Trp Phe Cys Tyr Gly Tyr Phe Thr Lys Asp 180 185 190Pro Tyr Val Met Tyr Pro Asn Val Gly Gly Phe Phe Phe Ser Cys Val 195 200 205Gln Met Gly Leu Tyr Phe Tyr Tyr Arg Arg Pro Ser Asn Ala Ala Val 210 215 220Leu Pro Thr Thr Ala Asp Gly Ala Thr Gly Gly Gly Ala Val Gln Ala225 230 235 240Gln Val Ile Glu Leu Pro Pro His Ala Val Ala Ile Leu Ser Val Ser 245 250 255Asn Ile Pro Ile Leu Gly Met His Lys Ile Glu Val Met Ala Ala Pro 260 265 270Glu Gln Gln Asp Ala Lys Ala Ala Asp Ile Val Asp Lys Ala Ala Pro 275 280 285Ala Pro Glu Ala Val Glu Ile Ala Gly Thr Val 290 295105309PRTBrachypodium distachyon 105Met Ala Phe Leu Asn Met Glu Gln His Thr Trp Ala Phe Thr Phe Gly1 5 10 15Ile Leu Gly Asn Ile Ile Ser Leu Met Val Phe Leu Ser Pro Leu Pro 20 25 30Thr Phe Tyr Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Thr Pro Tyr Val Val Thr Leu Phe Ser Cys Leu Leu Trp Met Tyr Tyr 50 55 60Ala Phe Leu Lys Ser Gly Ala Glu Leu Leu Leu Thr Ile Asn Gly Val65 70 75 80Gly Cys Gly Ile Glu Thr Leu Tyr Ile Ala Met Tyr Leu Ile Tyr Ala 85 90 95Pro Lys Ser Ala Arg Leu Leu Thr Ala Lys Leu Phe Leu Gly Leu Asp 100 105 110Val Gly Leu Phe Gly Leu Ile Ala Leu Val Thr Met Leu Val Ser Ala 115 120 125Gly Thr Leu Arg Val Gln Ile Val Gly Trp Ile Cys Val Ala Val Ala 130 135 140Leu

Gly Val Phe Ala Ala Pro Leu Ser Ile Ile Arg Leu Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Ile Ser Leu Ser Phe Phe Leu Val 165 170 175Leu Ser Ala Val Ile Trp Phe Ala Tyr Gly Leu Leu Lys Lys Asp Val 180 185 190Phe Val Ala Val Pro Asn Val Leu Gly Phe Val Phe Gly Val Ala Gln 195 200 205Met Ala Leu Tyr Met Ala Tyr Arg Asn Lys Ser Pro Ala Ile Thr Val 210 215 220Val His Gln Glu Met Lys Leu Pro Glu His Val Lys Glu Val Thr Thr225 230 235 240Asn Thr Lys Leu Gly Gly Ala Pro Thr Glu Gly Arg Ile Ser Cys Gly 245 250 255Ala Glu Val His Pro Ile Asp Val Met Pro Thr Ser Ala Ala Ala Gly 260 265 270Ala Asp Glu Gln Ala Ile Asn Val Glu Glu Ala Ala Ala Gly Arg Asp 275 280 285Asp His Asn Met Leu Arg Pro Glu Gln Val Ile Lys Pro Asp Met Ala 290 295 300Ile Val Val Glu Val305106300PRTBrachypodium distachyon 106Met Ala Gly Leu Ser Leu Gln His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Thr Tyr Leu Ala Pro Leu Ser Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Asn Lys Ser Thr Gln Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Leu Lys Ser Asp Gly Cys Leu Leu Ile Thr Ile Asn Thr Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Ile Val Val Tyr Leu Ala Tyr Ala Pro 85 90 95Lys Gln Ala Lys Leu Phe Thr Ala Lys Ile Leu Leu Leu Leu Asn Val 100 105 110Gly Val Phe Gly Met Ile Leu Leu Leu Thr Leu Leu Leu Ser Glu Gly 115 120 125Glu Lys Arg Val Val Met Leu Gly Trp Val Cys Val Gly Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Val Ile Arg Leu Val Val Arg Thr145 150 155 160Arg Ser Val Glu Phe Met Pro Phe Asn Leu Ser Leu Ser Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Ala Phe Gly Val Ile Gln Met 195 200 205Gly Leu Tyr Ala Leu Tyr Arg Asn Ser Thr Pro Arg Pro Val Thr Lys 210 215 220Glu Val Asp Ala Glu Ser His Asp Gly Ala Ala Pro Lys Val Pro Glu225 230 235 240His Val Val Asn Ile Gly Lys Leu Gly Ala Val Glu Leu Lys Thr Thr 245 250 255Glu Val Phe Ile His Pro Ala Ile Glu Ser Pro Pro Thr Lys Glu Asn 260 265 270Gly Val Ala His Gly Ala Glu Gln Ser Arg Glu Gly Gly Val Val Val 275 280 285Glu Lys Val Asp Glu Ala Ser Gln Val Glu Gln Val 290 295 300107292PRTBrachypodium distachyon 107Met Ala Gly Leu Ser Leu Glu His Pro Trp Ala Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn Val Ile Ser Phe Met Ser Tyr Leu Ala Pro Ile Pro Thr 20 25 30Phe Ile Arg Ile Tyr Lys Ser Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Leu Val Lys Ser Asn Glu Ser Leu Leu Ile Thr Ile Asn Ala Ala Gly65 70 75 80Cys Val Ile Glu Thr Ile Tyr Val Val Met Tyr Phe Val Tyr Ala Pro 85 90 95Arg Lys Ala Lys Leu Phe Thr Ala Lys Ile Met Leu Leu Leu Asn Gly 100 105 110Gly Val Phe Gly Val Ile Leu Phe Cys Thr Leu Phe Leu Ala His Gly 115 120 125Glu Lys Arg Val Val Ser Leu Gly Trp Ile Cys Val Ala Phe Ser Val 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Ile Gly Arg Val Ile Lys Thr145 150 155 160Arg Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Leu Ser Leu Thr Leu 165 170 175Ser Ala Val Val Trp Phe Leu Tyr Gly Leu Leu Ile Lys Asp Lys Tyr 180 185 190Val Ala Leu Pro Asn Ile Leu Gly Phe Ser Phe Gly Val Val Gln Met 195 200 205Ala Leu Tyr Met Phe Tyr Met Asn Lys Thr Pro Ile Val Arg Gly Asp 210 215 220Gly Lys Glu Gly Lys Leu Pro Ala Ala Glu Glu His Val Val Val Asn225 230 235 240Met Ala Lys Leu Gly Gly Gly Ala Thr Pro Asp Asn Lys Asn Cys Gly 245 250 255Ser Glu Val Tyr Pro Val Glu Val Lys Ala Leu Pro Lys Ser Cys Ala 260 265 270Ala Gly Val Asp Arg Pro Leu Val Asp Pro Thr Ala Arg Pro Ala Thr 275 280 285Val Glu Val Val 290108291PRTBrachypodium distachyon 108Met Ala Gly Ala Leu Phe Ser Met Ala His Pro Trp Ala Ser Ala Phe1 5 10 15Gly Ile Leu Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Thr 20 25 30Pro Thr Phe Leu Arg Val Tyr Arg Lys Lys Ser Thr Glu Gly Phe Ser 35 40 45Ser Val Pro Tyr Val Val Ala Leu Phe Ser Cys Thr Leu Trp Ile Leu 50 55 60Tyr Ala Leu Val Lys Thr Asn Ser Ser Pro Leu Leu Thr Ile Asn Ala65 70 75 80Phe Gly Cys Val Val Glu Ala Ala Tyr Ile Val Leu Tyr Leu Val Tyr 85 90 95Ala Pro Arg Pro Ala Arg Leu Arg Thr Leu Ala Ser Phe Leu Leu Leu 100 105 110Asn Val Ala Ala Phe Ser Leu Ile Val Ala Val Thr Val Phe Leu Val 115 120 125Ala Pro Met His Arg Val Lys Val Leu Gly Ser Ile Cys Leu Ala Phe 130 135 140Ser Met Ala Val Phe Val Ala Pro Leu Ser Val Ile Phe Val Val Ile145 150 155 160Lys Thr Lys Ser Ala Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu 165 170 175Thr Leu Ser Ala Val Ala Trp Phe Phe Tyr Gly Leu Phe Thr Lys Asp 180 185 190Ile Tyr Val Thr Leu Pro Asn Val Gly Gly Phe Phe Phe Gly Ile Ala 195 200 205Gln Met Thr Leu Tyr Phe Cys Tyr Arg Lys Pro Gly Thr Ser Ala Leu 210 215 220Val Leu Pro Thr Ser Ile Asp Asp Val Ser Thr Glu Pro Ala Ala Ser225 230 235 240Ala Ala Ala Asp Gln Glu Val Glu Leu Pro Ala Gly Thr His Pro Ala 245 250 255Val Ala Met Leu Thr Val Ser Thr Leu Pro Val Leu Ala Glu Leu Gln 260 265 270Lys Met Glu Gln Glu Ile Gly Thr Pro Thr Pro Arg Lys Gly Tyr Ile 275 280 285Lys Ala Phe 290109292PRTCucumis sativus 109Met Ala Ile Ser Pro Gln Thr Leu Ala Phe Val Phe Gly Leu Leu Gly1 5 10 15Asn Ile Ile Ser Phe Met Val Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Lys Ile Tyr Lys Lys Lys Ser Ala Glu Gly Tyr Gln Ser Val Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Leu Leu 50 55 60Lys Thr Asn Ala Thr Phe Leu Ile Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Ile Glu Ser Leu Tyr Ile Leu Leu Phe Ile Ile Tyr Ala Pro Thr Lys 85 90 95Leu Arg Phe Gln Thr Ala Lys Val Ile Phe Leu Leu Asn Val Leu Gly 100 105 110Phe Gly Leu Met Leu Ala Leu Thr Leu Val Leu Ala Lys Gly Glu Lys 115 120 125Arg Leu Lys Val Leu Gly Trp Ile Cys Leu Val Phe Asn Leu Ser Val 130 135 140Phe Ala Ala Pro Leu Phe Ile Met Gly Lys Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Phe Ala Leu Ser Phe Phe Leu Thr Leu Asn Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Tyr Tyr Ile Ala 180 185 190Leu Pro Asn Val Val Gly Phe Val Phe Gly Ile Ile Gln Met Ile Leu 195 200 205Tyr Val Ile Val Lys His Ile Gly Asn Lys Ser Arg Ile Pro Val Lys 210 215 220Asp Glu Lys Ala Ala Ala Pro Pro Gln Leu His Glu Leu Ser Glu Gln225 230 235 240Ile Ile Asp Ala Val Lys Leu Gly Thr Met Val Cys Thr Glu Leu Asn 245 250 255Pro Val Pro Val Thr Val Leu Gln Pro Asn Met Asp Val Val Asp Ala 260 265 270Val Val Glu Ala Val Ile Asp Asn Ile Gln Lys Lys Lys Asp Gln Asp 275 280 285Ile Ile Thr Asn 290110302PRTCucumis sativus 110Met Ala Leu Ser Phe Met Asn His Asn Pro Trp Ile Phe Ala Phe Gly1 5 10 15Leu Leu Gly Asn Ile Phe Ser Phe Ile Val Phe Leu Ala Pro Val Pro 20 25 30Thr Phe Ile Arg Val Cys Arg Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Ile Pro Tyr Val Val Ala Leu Phe Ser Ala Leu Leu Leu Ile Tyr Tyr 50 55 60Ser Thr Leu Asn Ala Asp Glu Phe Phe Leu Met Thr Ile Asn Ser Val65 70 75 80Gly Cys Phe Ile Glu Thr Ile Tyr Ile Ala Leu Tyr Ile Ala Tyr Ala 85 90 95Pro Lys Lys Ala Arg Ile Phe Thr Val Arg Phe Val Leu Leu Leu Asp 100 105 110Val Val Gly Phe Cys Ser Ile Leu Val Val Thr Gln Phe Leu Val Lys 115 120 125Arg Ala Tyr Arg Ala Arg Val Ile Gly Phe Ile Cys Gly Gly Leu Ser 130 135 140Val Ser Val Phe Ala Ala Pro Leu Ser Ile Met Lys Arg Val Ile Arg145 150 155 160Thr Arg Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu Thr 165 170 175Leu Ser Ala Val Met Trp Leu Cys Tyr Gly Leu Phe Leu Lys Asp Leu 180 185 190Tyr Val Ala Leu Pro Asn Thr Leu Gly Phe Thr Phe Gly Met Ala Gln 195 200 205Met Ile Leu Tyr Ala Ile Tyr Arg Asn Ala Lys Pro Leu Pro Ser Glu 210 215 220Glu Lys Leu Pro Gln His Lys Ala Asp Ile Glu Thr Gln Ile Val Ile225 230 235 240Thr Ala Thr Pro Thr Asn Pro Asp Asp His Gln Gly Asp Glu His Gln 245 250 255Asn Gln Asp Gln Val Ile Asn Val Pro Pro Pro Pro Pro Gln Ser Asn 260 265 270Thr Asn His Ala Pro Ser Val Cys Asn Asn Asn Asp Lys Tyr Cys Met 275 280 285Asp Asn Asn Met Ala Pro Pro Pro Met Val Lys Cys Glu Ala 290 295 300111236PRTCucumis sativus 111Met Asn Gly Leu Ser Val His Gln Leu Gln Phe Ile Phe Gly Leu Leu1 5 10 15Gly Asn Ile Ile Ser Phe Leu Val Phe Leu Ala Pro Met Pro Thr Phe 20 25 30Trp Thr Ile Tyr Lys Lys Lys Thr Ser Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Val Val Ala Leu Met Ser Ala Met Leu Leu Leu Tyr Tyr Ala Ala 50 55 60Leu Lys Thr Asn Ala Tyr Leu Leu Val Ser Ile Asn Ser Phe Gly Cys65 70 75 80Val Ile Glu Val Ile Tyr Ile Ala Leu Tyr Leu Phe Tyr Ala Pro Lys 85 90 95Lys Gln Lys Ile Phe Thr Leu Lys Leu Phe Ile Ile Phe Asn Leu Gly 100 105 110Phe Ser Gly Val Met Val Gly Gly Thr Met Phe Phe Leu His Gly Met 115 120 125Lys Arg Thr Asn Ala Val Gly Trp Ile Cys Ala Ala Phe Asn Leu Ser 130 135 140Val Phe Ala Ser Pro Leu Ser Ile Met Lys Arg Val Ile Thr Thr Lys145 150 155 160Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser 165 170 175Ala Thr Met Trp Phe Phe Tyr Gly Phe Phe Ile Lys Asp Leu Phe Ile 180 185 190Ala Leu Pro Asn Val Val Gly Phe Leu Leu Gly Met Val Gln Met Ile 195 200 205Met Tyr Ile Tyr Ile Leu Leu Leu Leu Leu Leu His Tyr Thr Thr Pro 210 215 220His Ala Asp Val Leu Gln Ile Phe Ser Leu Leu Ile225 230 235112274PRTCucumis sativus 112Met Lys Gly Leu Ser Val His Gln Leu Gln Phe Ile Phe Gly Leu Leu1 5 10 15Gly Asn Ile Ile Ser Phe Met Val Phe Leu Ala Pro Val Pro Thr Phe 20 25 30Trp Thr Val Tyr Lys Lys Lys Thr Ser Glu Gly Phe Gln Cys Ile Pro 35 40 45Tyr Val Val Ala Leu Met Ser Ala Met Leu Leu Leu Tyr Tyr Ala Val 50 55 60Leu Lys Thr Asn Ala Tyr Leu Leu Ile Ser Ile Asn Ser Phe Gly Cys65 70 75 80Val Ile Glu Leu Ile Tyr Ile Ala Leu Tyr Phe Tyr Tyr Ala Pro Lys 85 90 95Lys Leu Lys Ile Phe Thr Leu Lys Leu Leu Met Ile Leu Asn Leu Gly 100 105 110Ser Tyr Gly Val Met Val Gly Gly Thr Met Leu Ile Leu His Gly Asn 115 120 125Lys Arg Thr His Ala Val Gly Trp Ile Cys Ala Ala Phe Asn Leu Ala 130 135 140Val Phe Ala Ser Pro Leu Ala Ile Met Lys Arg Val Ile Thr Thr Lys145 150 155 160Ser Val Glu Tyr Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser 165 170 175Ala Thr Met Trp Phe Phe Tyr Gly Phe Phe Ile Lys Asp Leu Phe Ile 180 185 190Ala Leu Pro Asn Ile Val Gly Phe Leu Leu Gly Met Val Gln Met Ile 195 200 205Met Tyr Met Ile Tyr Lys Asp Arg Lys Gly Asn Ser Leu Glu Glu Lys 210 215 220Leu Glu Glu Gly Gly Lys Lys Tyr Glu Val Asp Asp Gln Ser Leu Ser225 230 235 240Lys Tyr Lys Gly Gln Ile Arg Arg Ile Leu Asp Leu Val Leu Glu Glu 245 250 255Tyr Arg Phe Ser Arg Glu Asn Tyr Asn Ala Leu Asp Val Val Glu Pro 260 265 270Thr Thr113277PRTCucumis sativus 113Met Thr Ile Phe His Ser Pro His Leu Leu Val Phe Thr Phe Gly Leu1 5 10 15Leu Gly Asn Ile Ile Ser Phe Phe Val Tyr Leu Ala Pro Leu Pro Thr 20 25 30Phe Tyr Arg Ile Trp Gln Lys Lys Ser Thr Glu Gly Phe His Ala Leu 35 40 45Pro Tyr Leu Val Ala Leu Phe Ser Ser Ala Leu Trp Leu Cys Tyr Ala 50 55 60Phe Leu Lys Thr Asn Thr Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly65 70 75 80Cys Val Ile Glu Phe Leu Tyr Phe Ile Val Phe Ile Val Phe Ala Ala 85 90 95Asn Ser Val Arg Met Leu Thr Ile Arg Ile Phe Ala Met Met Asn Met 100 105 110Gly Leu Phe Gly Leu Ile Leu Val Ala Ile His Phe Ile Pro Asn Pro 115 120 125Ser Asn Arg Thr Asp Val Met Gly Trp Ile Cys Val Ala Val Ser Val 130 135 140Ser Val Phe Ala Ala Pro Leu Ser Ile Leu Arg Gln Val Met Thr Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Thr Leu Ser Phe Phe Leu Thr Leu 165 170 175Ser Ala Ile Met Trp Phe Ala Tyr Gly Leu Leu Leu Asn Asp Ile Cys 180 185 190Ile Ala Ile Pro Asn Val Val Gly Phe Ile Leu Gly Leu Leu Gln Met 195 200 205Val Val Tyr Ala Ile Tyr Arg Lys Arg Lys Ile Val Ile Met Glu Glu 210 215 220Lys Lys Gln Pro Glu Gln Val Val Leu Lys Ser Ile Ala Val Ser Glu225 230 235 240Val Phe Ala Met Lys Lys Pro Asn Gly Asn Asp Ala Gln Leu Lys Glu 245 250 255Val Ile Ile Ile Lys Gln Glu Ala Gln Glu Asp Asp Lys Leu Ser Cys 260 265 270Asp Lys Ile Asn Thr 275114291PRTCucumis sativus 114Met Ala Leu Phe Asp Thr His His Pro Gly Val Phe Ala Phe Gly Leu1 5 10 15Leu Gly Asn

Ile Ile Ser Phe Ile Val Phe Leu Ala Pro Val Pro Thr 20 25 30Phe Met Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Val 35 40 45Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr Ala 50 55 60Ser Phe Asn Ser Asn Glu Thr Leu Leu Ile Thr Ile Asn Ser Val Gly65 70 75 80Cys Leu Ile Glu Thr Leu Tyr Ile Ala Ile Phe Ile Val Phe Ala Pro 85 90 95Lys Gln Ile Arg Val Ser Thr Leu Arg Phe Val Leu Leu Leu Asn Phe 100 105 110Gly Gly Phe Cys Ile Ile Leu Leu Val Thr His Phe Leu Val His Gly 115 120 125Ser Asn Gln Val Lys Val Val Gly Trp Ile Cys Val Ala Phe Ser Val 130 135 140Ser Val Phe Ala Ala Pro Leu Thr Ile Met Arg Leu Val Ile Arg Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu 165 170 175Ser Ala Ile Thr Trp Leu Leu Tyr Gly Val Phe Leu Lys Asp Ile Tyr 180 185 190Val Ala Leu Pro Asn Val Leu Gly Phe Ile Phe Gly Val Ala Gln Met 195 200 205Ile Leu Tyr Leu Ile Tyr Arg Lys Tyr Glu Ile Ala Ile Ala Lys Glu 210 215 220Met Lys Leu Pro Glu Gln Thr Thr Val Asp Ile Val Met Lys Gln Lys225 230 235 240Gln Asp Ser Ser Val Glu Ala Ile Glu Val Ile Ile Lys Thr Asn Ile 245 250 255Glu Glu Ile Glu Leu Ser Asn Gly Asn Asn Asn Asn Asp Asn Asp Lys 260 265 270His Asn His Lys Thr Leu Glu Val Ser His Gln Ile Thr Asp His Leu 275 280 285Asn His Val 290115295PRTCucumis sativus 115Met Ala Leu Ser Phe Asn Thr His Asn Pro Ala Ala Phe Thr Phe Gly1 5 10 15Leu Leu Gly Asn Ile Ile Ser Phe Ile Val Phe Leu Ala Pro Val Pro 20 25 30Thr Phe Met Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Ile Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr 50 55 60Ala Ser Phe Asn Pro Asn Glu Thr Leu Leu Ile Thr Ile Asn Ser Val65 70 75 80Gly Cys Leu Ile Glu Thr Ile Tyr Leu Ala Ile Phe Ile Val Phe Ala 85 90 95Pro Lys Gln Ile Arg Val Ser Thr Leu Arg Phe Val Leu Leu Leu Asn 100 105 110Phe Gly Gly Phe Cys Ile Ile Leu Leu Val Thr His Phe Leu Val His 115 120 125Gly Ser Asn Arg Val Lys Val Val Gly Trp Ile Cys Val Ala Phe Ser 130 135 140Ile Ser Val Phe Ala Ala Pro Leu Thr Ile Ile Arg Leu Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Tyr Leu Ser Phe Phe Leu Thr 165 170 175Leu Ser Ala Thr Ser Trp Leu Leu Tyr Gly Val Phe Leu Lys Asp Ile 180 185 190Tyr Ile Ala Val Pro Asn Ile Pro Gly Phe Met Phe Gly Ile Ala Gln 195 200 205Met Ile Leu Tyr Leu Ile Tyr Lys Lys Arg Glu Thr Ala Met Glu Met 210 215 220Gln Leu Pro Gln His Ser Thr Asp Asn Ile Val Ile Val Ser Ala Ala225 230 235 240Thr Asn Ser Asp Lys Gln Lys Gln His Ser Ser Ser Leu Pro Ser Asn 245 250 255Asn Leu Val Gly Ala Ala Val Asp Asp Asp Asp Val Thr Thr Thr Thr 260 265 270Lys Asn Gly Ile Asp Pro Ile Asn Asn Leu Glu Gln Asn His Gln Val 275 280 285Lys Asp Gln Leu Asn His Val 290 295116294PRTCucumis sativus 116Met Ala Leu Ser Phe Asn Thr His Asn Pro Ala Ala Phe Thr Phe Gly1 5 10 15Leu Leu Gly Asn Ile Ile Ser Phe Ile Val Phe Leu Ala Pro Val Pro 20 25 30Thr Phe Met Arg Ile Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser 35 40 45Ile Pro Tyr Val Val Ala Leu Phe Ser Ala Met Leu Trp Leu Tyr Tyr 50 55 60Ala Ser Phe Asn Pro Asn Glu Thr Leu Leu Ile Thr Ile Asn Ser Val65 70 75 80Gly Cys Leu Ile Glu Thr Ile Tyr Leu Ala Ile Phe Ile Val Phe Ala 85 90 95Pro Lys Gln Ile Arg Val Ser Thr Leu Arg Phe Val Leu Leu Leu Asn 100 105 110Phe Gly Gly Phe Cys Ile Ile Leu Leu Val Thr His Phe Leu Val His 115 120 125Gly Ser Asn Arg Val Lys Val Val Gly Trp Ile Cys Val Ala Phe Ser 130 135 140Ile Ser Val Phe Ala Ala Pro Leu Thr Ile Ile Arg Leu Val Ile Arg145 150 155 160Thr Lys Ser Val Glu Phe Met Pro Phe Tyr Leu Ser Phe Phe Leu Thr 165 170 175Leu Ser Ala Thr Ser Trp Leu Leu Tyr Gly Val Phe Leu Lys Asp Ile 180 185 190Tyr Ile Ala Val Pro Asn Ile Pro Gly Phe Met Phe Gly Ile Ala Gln 195 200 205Met Ile Leu Tyr Leu Ile Tyr Lys Lys Arg Glu Thr Ala Met Glu Met 210 215 220Gln Leu Pro Gln His Ser Thr Asp Asn Thr Val Ile Val Ser Ala Ala225 230 235 240Thr Asn Ser Asp Lys Gln Lys Gln His Ser Ser Ser Leu Pro Ser Asn 245 250 255Asn Leu Val Gly Ala Ala Val Asp Asp Asp Val Thr Thr Thr Thr Lys 260 265 270Asn Gly Ile Asp Pro Ile Asn Asn Leu Glu Glu Asn His Gln Val Lys 275 280 285Asp Gln Leu Asn His Val 290117300PRTSolanum lycopersicum 117Met Ala Val Asp Phe Gly His Tyr Ala Phe Ala Phe Gly Val Leu Gly1 5 10 15Asn Ile Ile Ser Phe Ile Val Phe Leu Ser Pro Ile Pro Thr Phe Tyr 20 25 30Ser Ile Tyr Lys Lys Lys Ser Thr Glu Gly Tyr Gln Ser Ile Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Leu Leu 50 55 60Lys Ser Asn Met Pro Leu Leu Ile Thr Ile Asn Ser Phe Gly Met Phe65 70 75 80Ile Glu Thr Ile Tyr Val Gly Phe Tyr Leu Phe Tyr Ala Pro Lys Lys 85 90 95Ala Arg Val His Thr Ile Lys Met Leu Met Leu Ser Val Val Gly Gly 100 105 110Phe Gly Ala Ile Val Leu Val Thr Glu Phe Leu Phe Lys Gly Val Val 115 120 125Arg Gly Gln Ile Val Gly Trp Ile Cys Leu Ile Phe Ser Leu Cys Val 130 135 140Phe Val Ala Pro Leu Gly Ile Val Arg Gln Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Leu Leu Leu Ser Val Phe Leu Thr Leu Ser Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Ile Asn Ile Ala 180 185 190Ala Pro Asn Val Leu Gly Phe Ile Phe Gly Ile Leu Gln Ile Ile Leu 195 200 205Tyr Ala Ile Tyr Ser Lys Lys Glu Lys Ser Ile Ile Lys Glu Gln Lys 210 215 220Leu Pro Glu Ile Gln Lys Thr Glu Val Ile Val Lys Asp Glu Asn Met225 230 235 240Asn Ala Asn Lys Lys Leu Pro Glu Leu Thr Gln Glu Gln Ile Ile Asp 245 250 255Ile Val Lys Leu Ala Gly Leu Leu Val Val Thr Asp Lys Thr Asn Val 260 265 270Ala Thr Cys Pro Asn Asp Thr Asn Cys Gly Val Lys Ala Val Asn Lys 275 280 285Ile Glu Asn Met Pro Lys Leu Gln Thr Val Ala Thr 290 295 300118296PRTSolanum lycopersicum 118Met Ala Phe Ser Ala Asp His Trp Ala Phe Val Phe Gly Val Leu Gly1 5 10 15Asn Ile Val Ser Phe Ile Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Thr Ile Tyr Lys Lys Lys Thr Ala Glu Gly Tyr Gln Ser Ile Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Phe Leu 50 55 60Lys Thr Asn Thr Thr Leu Leu Ile Thr Ile Asn Thr Phe Gly Val Phe65 70 75 80Val Glu Thr Ile Tyr Val Val Phe Tyr Leu Ile Tyr Ala Pro Lys Lys 85 90 95Ser Arg Val Gln Thr Ile Lys Met Leu Ser Leu Phe Val Val Gly Gly 100 105 110Phe Gly Ala Ile Ile Leu Val Thr Gln Phe Leu Phe Lys Gly Val Ile 115 120 125Arg Gly Gln Val Val Gly Trp Ile Cys Leu Ile Phe Ser Leu Cys Val 130 135 140Phe Val Ala Pro Leu Gly Ile Val Arg Lys Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Leu Leu Leu Ser Val Phe Leu Thr Leu Ser Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Ile Asn Ile Ala 180 185 190Ala Pro Asn Ile Leu Gly Phe Ile Phe Gly Val Leu Gln Met Ile Leu 195 200 205Tyr Val Ile Tyr Ser Lys Lys Glu Lys Ala Ile Leu Lys Glu Gln Lys 210 215 220Leu Pro Glu Ile Gln Lys Gly Glu Val Ile Val Lys Asp Glu Asn Met225 230 235 240Asn Ala Asp Lys Lys Phe Pro Glu Leu Thr Gln Glu Gln Ile Ile Asp 245 250 255Ile Val Arg Leu Gly Leu Met Val Cys Lys Gly Lys Val His Val Ala 260 265 270Thr Cys Pro His Gly Thr Thr Cys Glu Pro Lys Val Asp Glu Asn Glu 275 280 285Pro Lys Leu Gln Thr Val Glu Val 290 295119298PRTSolanum lycopersicum 119Met Val Phe Asn His Trp Ala Phe Ala Phe Gly Val Leu Gly Asn Ile1 5 10 15Val Ser Phe Ile Val Phe Leu Ser Pro Ile Pro Thr Phe Tyr Asn Ile 20 25 30Tyr Lys Lys Lys Ser Thr Glu Gly Tyr Gln Ser Ile Pro Tyr Val Val 35 40 45Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Leu Leu Lys Ser 50 55 60Asn Met Pro Leu Leu Ile Thr Ile Asn Ser Phe Gly Met Phe Ile Glu65 70 75 80Thr Ile Tyr Val Gly Leu Tyr Leu Leu Tyr Ala Pro Asn Lys Ala Arg 85 90 95Val His Thr Ile Lys Met Leu Met Leu Ser Val Val Gly Gly Phe Gly 100 105 110Ala Ile Val Leu Ile Thr Glu Phe Leu Phe Lys Gly Val Val Arg Gly 115 120 125Gln Ile Val Gly Trp Ile Cys Leu Ile Phe Ser Leu Cys Val Phe Val 130 135 140Ala Pro Leu Gly Ile Val Arg Lys Val Ile Lys Thr Lys Ser Val Glu145 150 155 160Tyr Met Pro Leu Leu Leu Ser Val Phe Leu Thr Leu Ser Ala Val Met 165 170 175Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Ile Asn Ile Ala Ala Pro 180 185 190Asn Val Leu Gly Phe Ile Phe Gly Ile Leu Gln Ile Val Leu Tyr Ala 195 200 205Ile Tyr Ser Lys Lys Glu Lys Val Ile Leu Lys Glu Gln Lys Leu Pro 210 215 220Glu Ile Gln Thr Pro Ala Val Ile Val Lys Asn Glu Asn Met Met Asn225 230 235 240Thr Thr Lys Lys Leu Pro Glu Leu Thr Gln Glu Gln Ile Ile Asp Ile 245 250 255Val Lys Leu Gly Leu Leu Val Cys Ser Asp Lys Thr Gln Val Ala Thr 260 265 270Cys Pro Asn Asp Thr Asn Cys Gly Val Lys Asp Thr Asn Asn Asn Val 275 280 285Val Asn Met Pro Lys Leu Gln Thr Val Ala 290 295120282PRTSolanum lycopersicum 120Met Ala Ile Ala Gly His Trp Ala Phe Val Phe Gly Val Leu Gly Asn1 5 10 15Ile Ile Ser Phe Ile Val Phe Leu Ser Pro Ile Pro Thr Phe Asn Lys 20 25 30Ile Tyr Lys Lys Lys Ser Thr Glu Gly Tyr Gln Ser Ile Pro Tyr Val 35 40 45Ile Ala Leu Phe Ser Cys Met Leu Trp Ile Tyr Tyr Ala Phe Leu Lys 50 55 60Thr Asn Thr Thr Leu Leu Ile Thr Ile Asn Ser Phe Gly Met Leu Ile65 70 75 80Glu Thr Ile Tyr Val Ser Leu Phe Leu Tyr Tyr Ala Pro Lys Lys Ala 85 90 95Arg Val Asn Thr Val Lys Met Leu Leu Leu Thr Val Val Gly Gly Phe 100 105 110Gly Ala Ile Ile Leu Val Thr Gln Phe Leu Phe Lys Gly Val Val Arg 115 120 125Gly Gln Ile Val Gly Trp Ile Cys Leu Ile Phe Ser Leu Cys Val Phe 130 135 140Val Ala Pro Leu Gly Ile Val Arg Gln Val Ile Lys Thr Lys Ser Val145 150 155 160Glu Tyr Met Pro Ile Leu Leu Ser Val Phe Leu Thr Ile Ser Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Val Asn Ile Ala Ala 180 185 190Pro Asn Ile Leu Gly Phe Ile Phe Gly Ile Leu Gln Met Ile Leu Tyr 195 200 205Ala Met Tyr Arg Lys Lys His Lys Pro Ile Val Asn Val Asn Val Glu 210 215 220Val Gln Asn Pro Val Ile Ile Leu Asp Asp Asn Lys Lys Ile Pro Glu225 230 235 240Leu Thr Glu Glu Gln Ile Ile Asp Ile Val Lys Leu Gly Lys Leu Val 245 250 255Cys Ser Gly Lys Ile Gln Met Ala Ser Thr Leu Asn Gln Asn Ala Ser 260 265 270Lys Glu Val Lys Gln Gln Thr Glu Glu Ala 275 280121295PRTSolanum lycopersicum 121Met Thr Thr His Leu Ala Phe Val Phe Gly Leu Leu Gly Asn Ile Val1 5 10 15Ser Phe Met Val Tyr Leu Ala Pro Val Pro Thr Phe Tyr Lys Ile Tyr 20 25 30Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Val Pro Tyr Val Val Gly 35 40 45Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Leu Lys Pro Asp 50 55 60Thr Thr Leu Leu Ile Thr Ile Asn Ser Val Gly Cys Phe Ile Gln Thr65 70 75 80Phe Tyr Ile Cys Phe Phe Leu Phe Tyr Ala Thr Lys Lys Ala Lys Met 85 90 95Asp Thr Met Lys Leu Leu Leu Ser Met Asn Val Val Gly Leu Gly Leu 100 105 110Ile Ile Phe Leu Thr Gln Phe Phe Ala Lys Gly Ser Asn Arg Ala Gln 115 120 125Ile Val Gly Trp Ile Cys Leu Ile Phe Ser Phe Cys Val Phe Val Ala 130 135 140Pro Leu Gly Val Leu Arg Gln Val Ile Arg Thr Lys Ser Val Glu Tyr145 150 155 160Met Pro Phe Gln Leu Ser Phe Phe Leu Thr Leu Ser Ala Val Met Trp 165 170 175Phe Leu Tyr Gly Leu Leu Arg Lys Asp Tyr Asn Ile Ala Ile Pro Asn 180 185 190Val Leu Gly Phe Ser Leu Gly Val Ile Gln Met Thr Leu Tyr Leu Ile 195 200 205Tyr Lys Asn Ala Lys Lys Val Thr Lys Glu Val Lys Leu Glu Val Thr 210 215 220Glu Ile Val Ala Asp Asp Lys Glu Leu Lys Leu Ser Glu Glu Ile Leu225 230 235 240Lys Asp Gln Ile Ile Asp Val Val Lys Leu Ser Ala Ile Val Cys Ser 245 250 255Glu Ile Ile Pro Met Val Thr Gly Asn Glu Leu Lys Asn Glu Ile Asn 260 265 270Leu Pro Gln Leu Asn Val Ile Ile Asn Glu Asp Met Met Ile Lys Pro 275 280 285Lys Ala Ile Ile Glu Ala Ser 290 295122297PRTSolanum lycopersicum 122Met Ala Gly Ile Ser Gly His Trp Ala Phe Ala Phe Gly Val Leu Gly1 5 10 15Asn Ile Val Ser Phe Ile Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Lys Ile Tyr Lys Lys Lys Ser Thr Asp Gly Tyr Gln Ser Ile Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ser Met Leu Trp Ile Tyr Tyr Ala Phe Leu 50 55 60Lys Thr Asn Thr Thr Leu Leu Ile Thr Ile Asn Ser Phe Gly Val Phe65 70 75 80Ile Glu Thr Ile Tyr Val Gly Phe Tyr Leu Phe Tyr Ala Pro Lys Lys 85 90 95Asp Arg Val Gln Thr Ile Lys Met Leu Met Leu Ser Val Val Gly Gly 100 105 110Phe Gly Ala Ile Val Leu Ile Thr Glu

Phe Leu Phe Lys Gly Val Val 115 120 125Arg Gly Gln Ile Val Gly Trp Ile Cys Leu Ile Phe Ser Leu Cys Val 130 135 140Phe Val Ala Pro Leu Gly Ile Val Lys Gln Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Leu Leu Leu Ser Ile Phe Leu Thr Leu Ser Ala 165 170 175Val Val Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Ile Asn Ile Ala 180 185 190Ile Pro Asn Val Leu Gly Phe Ile Leu Gly Ile Leu Gln Met Val Leu 195 200 205Tyr Val Ile Tyr Asn Lys Lys Glu Lys Ala Ile Leu Lys Glu Gln Lys 210 215 220Leu Pro Glu Lys Leu Gln Asn His Met Ile Ile Ser Met Asp Glu Lys225 230 235 240Asn Lys Asn Ser Pro Gly Leu Thr Glu Glu Gln Ile Ile Asp Ile Val 245 250 255Lys Leu Gly Ser Leu Ile Ser Ser Gly Lys Ile His Ile Ala Ser Cys 260 265 270Leu His Asp Ala Met Cys Ala Ser Ala Lys Ile Glu Asn Thr Pro Asn 275 280 285Asn Leu Glu Thr Val Glu Ala Ile Asn 290 295123276PRTSolanum lycopersicum 123Met Thr Ser Val Ser His Thr His Pro Leu Val Tyr Thr Phe Gly Ile1 5 10 15Leu Gly Asn Leu Val Ser Phe Met Val Phe Ile Ala Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Val Lys Lys Lys Ser Ser Glu Gly Phe His Ser Leu 35 40 45Pro Tyr Val Val Gly Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala 50 55 60Met Val Lys Thr Asn Val Thr Leu Leu Ile Thr Ile Asn Ser Phe Gly65 70 75 80Cys Ile Ala Glu Thr Ile Tyr Val Ala Ile Tyr Phe Thr Tyr Ala Thr 85 90 95Lys Lys Ala Arg Met Lys Thr Leu Gly Leu Val Leu Leu Leu Asn Phe 100 105 110Gly Val Phe Gly Leu Ile Leu Phe Leu Thr Gln Ile Leu Cys Gln Gly 115 120 125Thr Lys Arg Ala Glu Val Ile Gly Trp Ile Cys Met Ala Phe Ser Ile 130 135 140Ser Val Phe Val Ala Pro Leu Ser Ile Met Gly Arg Val Ile Arg Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Asn Leu Ser Leu Ala Leu Thr Val 165 170 175Ser Ala Val Met Trp Phe Leu Tyr Gly Leu Leu Leu Lys Asp Val Tyr 180 185 190Val Ala Val Pro Asn Ile Pro Gly Met Ile Leu Gly Val Leu Gln Met 195 200 205Ile Leu Tyr Gly Ile Tyr Arg Asn Ser Lys Ser Asn Asn Val Ala Thr 210 215 220Glu Lys Glu Leu Pro Ile Val Val Lys Val Asp Gln Glu Gln Pro Thr225 230 235 240Lys Val Asn Ser Glu Val Tyr Pro Val Asn Ile Ser Ser Leu Asp Ser 245 250 255Glu Asn Gly Glu Ala Lys Asp Gly Lys Asn Leu Gln Asp Pro Gln Met 260 265 270Asn Ser Gln Val 275124287PRTSolanum lycopersicum 124Met Ala Gly Phe Ser Asp His Trp Thr Phe Ala Phe Gly Val Leu Gly1 5 10 15Asn Ile Ile Ser Phe Phe Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Asn Ile Tyr Lys Lys Lys Ser Thr Glu Gly Tyr Gln Ser Ile Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Leu 50 55 60Lys Thr Asn Thr Thr Leu Leu Val Thr Ile Asn Thr Phe Gly Cys Phe65 70 75 80Ile Glu Thr Leu Tyr Val Gly Phe Tyr Leu Phe Tyr Ala Pro Lys Lys 85 90 95Ala Arg Val Gln Thr Ile Lys Leu Leu Leu Leu Leu Val Val Gly Gly 100 105 110Phe Gly Ala Ile Ile Leu Ile Thr Gln Phe Leu Phe Lys Gly Ala Ile 115 120 125Arg Ala Gln Ile Val Gly Trp Ile Cys Leu Val Phe Ser Leu Cys Val 130 135 140Phe Val Ala Pro Leu Cys Ile Val Arg Gln Val Ile Lys Thr Lys Ser145 150 155 160Val Glu Tyr Met Pro Phe Leu Leu Ser Val Phe Leu Thr Leu Ser Ala 165 170 175Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Phe Asn Ile Ala 180 185 190Ile Pro Asn Val Leu Gly Phe Ile Phe Gly Ile Leu Gln Met Ile Leu 195 200 205Tyr Val Met Tyr Asn Lys Lys Glu Lys Val Val Ile Lys Glu Gln Asn 210 215 220Leu Pro Glu Leu Lys Asp His Val Ile Ile Ile Glu Asp Asp Lys Lys225 230 235 240Lys Leu Pro Glu Leu Ser Glu Glu Gln Ile Ile Asn Ile Ile Lys Leu 245 250 255Gly Ser Leu Val Tyr Ser Asp Lys Asn Tyr Gly Asn Leu Thr Glu Val 260 265 270Ala Lys Asn Asp Lys Ala Ile Ser Lys Leu Gln Thr Leu Glu Ala 275 280 285125286PRTSolanum lycopersicum 125Met Pro Leu Phe Thr Thr Leu Gln Leu Ala Phe Ala Phe Gly Ile Leu1 5 10 15Gly Asn Gly Val Ser Phe Leu Val Tyr Leu Ser Pro Leu Pro Thr Phe 20 25 30Tyr Arg Ile Phe Lys Arg Lys Ser Thr Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Ser Val Ser Leu Phe Ser Ala Met Leu Tyr Leu Tyr Tyr Ala Tyr 50 55 60Leu Lys Lys Asn Glu Ile Leu Leu Ile Thr Ile Asn Ser Phe Gly Thr65 70 75 80Gly Ile Gln Leu Ile Tyr Leu Thr Ile Phe Met Ile Tyr Ala Thr Lys 85 90 95Ser Ala Lys Ile Phe Ala Thr Lys Leu Leu Ile Gly Phe Asn Leu Val 100 105 110Ala Phe Gly Ala Ile Val Gly Leu Thr Tyr Val Phe Ala Asn Glu Asn 115 120 125Asp Leu Arg Ile Ser Ile Val Gly Trp Ile Cys Ala Val Phe Ser Val 130 135 140Ser Val Phe Ala Ala Pro Leu Ser Ile Met Arg Arg Val Ile Gln Thr145 150 155 160Lys Ser Val Glu Phe Met Pro Phe Pro Leu Ser Phe Phe Leu Thr Ile 165 170 175Cys Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Lys Lys Asp Met Tyr 180 185 190Ile Ala Met Pro Asn Ile Leu Gly Phe Ser Phe Gly Ile Ala Gln Met 195 200 205Ile Leu Tyr Ala Ile Tyr Arg Asn Arg Lys Gln Gln Val Leu Pro Asp 210 215 220Leu Ser Leu Met Asp Leu Lys Glu Ile Ala Ile Asp Met Lys Ala Val225 230 235 240Val Val Glu Ile Ile Gln Glu Asn Val Asp Asp Glu Asn Lys Asn Lys 245 250 255Ile Asn Lys Gln Glu Glu Val Val Ser Val Asp Glu Lys Lys Asp Val 260 265 270Glu Tyr Asp Lys Gln Asp Val Ala Leu Thr Thr Ser Asn Val 275 280 285126310PRTFragaria vesca 126Met Thr Ser Ser His Ser Pro Leu Ala Phe Ala Phe Gly Ile Leu Gly1 5 10 15Asn Ile Val Ser Phe Ile Val Phe Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Tyr Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Ile Pro Tyr 35 40 45Ile Phe Ala Leu Phe Ser Ala Thr Ile Trp Ile Tyr Tyr Ala Ser Leu 50 55 60Lys Ser Asp Glu Met Leu Leu Ile Thr Ile Asn Gly Phe Gly Cys Val65 70 75 80Ile Glu Thr Ile Tyr Ile Ala Met Tyr Ile Thr Tyr Ala Pro Lys Lys 85 90 95Ala Arg Val Asn Thr Leu Arg Leu Leu Leu Leu Val Asn Phe Gly Gly 100 105 110Phe Cys Leu Ile Leu Phe Leu Ser His Phe Leu Thr Gln Gly Pro Thr 115 120 125Arg Val Lys Val Leu Gly Trp Val Cys Val Ala Phe Ser Val Ser Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Met Arg Leu Val Ile Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Ser Leu Ser Phe Phe Leu Thr Leu Ser Ala 165 170 175Ile Met Trp Leu Phe Tyr Gly Leu Leu Leu Lys Asp Leu Tyr Val Ala 180 185 190Ala Pro Asn Ile Leu Gly Phe Ser Phe Gly Val Val Gln Met Ile Leu 195 200 205Tyr Ala Ala Tyr Arg Asn Lys Lys Thr Val Leu Val Asp Glu Glu Lys 210 215 220Val Pro Glu His Lys Thr Ala Asp Asp Phe Val Lys Gln Ile Asn Ile225 230 235 240Thr Val Leu Pro Ser Pro Lys Ala Gln Val Gln Val Lys Glu Glu Ala 245 250 255Val Ser Pro Ala Arg Ala Asp Ser Ser Asp Glu Ser Ser Asp Asp His 260 265 270Glu Gln Asn Val His Asp Gln Gln Arg Glu His Val Pro Gln Pro Cys 275 280 285His Val Asp Ala Gln Gly Ala Asp Leu Ile Ser Lys Gln Val Pro Ala 290 295 300Leu Val Gln Cys Glu Val305 310127292PRTFragaria vesca 127Met Ala Ile His His Pro Leu Thr Leu Ala Phe Gly Leu Leu Gly Asn1 5 10 15Ile Ile Ser Phe Met Val Phe Leu Ala Pro Met Pro Thr Phe Tyr Lys 20 25 30Ile Tyr Lys Lys Lys Thr Thr Glu Gly Phe Gln Ala Leu Pro Tyr Ala 35 40 45Val Ala Leu Phe Ser Cys Met Leu Trp Ile Tyr Tyr Ala Leu Leu Lys 50 55 60Gln Asp Ala Thr Phe Leu Ile Thr Ile Asn Ser Val Gly Cys Val Ile65 70 75 80Glu Thr Val Tyr Leu Ala Ile Phe Leu Phe Tyr Ser Pro Lys Lys Ala 85 90 95Arg Ile Ser Thr Val Lys Phe Leu Leu Leu Leu Asn Val Leu Gly Tyr 100 105 110Gly Leu Met Leu Val Leu Thr Leu Phe Leu Ala Lys Gly Glu Ile Arg 115 120 125Leu Lys Val Val Gly Trp Ile Cys Leu Val Phe Asn Leu Thr Val Phe 130 135 140Ala Ala Pro Leu Cys Ile Leu Lys Lys Val Ile Arg Thr Lys Ser Val145 150 155 160Glu Phe Met Pro Phe Pro Leu Ser Phe Phe Leu Thr Leu Gly Ala Val 165 170 175Met Trp Phe Phe Tyr Gly Phe Leu Leu Lys Asp Tyr Asn Ile Ala Phe 180 185 190Pro Asn Ile Leu Gly Phe Met Phe Gly Ile Ala Gln Met Val Leu Tyr 195 200 205Ile Val Tyr Lys Asn Ala Lys Lys Val Val Leu Leu Glu Glu Pro Ser 210 215 220Lys Val Gln Glu Leu Ser Asp His Ile Ile Glu Val Met Lys Ile Ser225 230 235 240Thr Met Val Cys Pro Asp Leu Thr Pro Val Val Leu Gln Pro Asn Asp 245 250 255Ile Asn Ile Asp Leu Ile Glu Val Ile Pro Leu Asp Pro Asn Asn Val 260 265 270Val Lys Gly Ile Asn Ala Glu Glu Ser Lys Glu Asp Met Asp Asp Ala 275 280 285Ser Thr Lys Val 290128291PRTFragaria vescamisc_feature(137)..(137)Xaa can be any naturally occurring amino acid 128Met Ser Met Tyr Asn Ser Gln His His Leu Ala Phe Ile Phe Val Val1 5 10 15Ile Gly Asp Val Ile Ser Phe Met Val Tyr Leu Ala Pro Val Pro Thr 20 25 30Phe Tyr Arg Ile Tyr Lys Lys Lys Thr Thr Glu Gly Phe Gln Ser Leu 35 40 45Pro Tyr Leu Val Ala Leu Phe Gly Ser Thr Leu Trp Leu Tyr Tyr Gly 50 55 60Ile Val Lys Gln Asn Met Val Leu Leu Ile Thr Ile Asn Thr Phe Gly65 70 75 80Ser Val Met Glu Thr Leu Tyr Ile Ala Met Tyr Ile Val Tyr Ala Thr 85 90 95Asn Ala Ser Arg Lys Leu Thr Ile Lys Leu Phe Gly Phe Met Asn Leu 100 105 110Gly Leu Phe Ser Leu Ile Val Val Cys Ile Ser Tyr Ala Val His Ser 115 120 125Glu Tyr Arg Ala Leu Val Leu Gly Xaa Ile Asn Val Ala Val Thr Ile 130 135 140Cys Ala Ser Ala Ala Pro Leu Ser Ile Val Ala Gln Val Ile Arg Thr145 150 155 160Gly Ser Val Glu Phe Met Pro Phe Thr Leu Ser Phe Phe Leu Thr Leu 165 170 175Ser Gly Val Leu Trp Phe Ser Tyr Gly Met Leu Leu Lys Asp Ile Phe 180 185 190Ile Ala Leu Pro Asn Gly Leu Gly Phe Val Leu Gly Leu Leu Gln Met 195 200 205Leu Phe Tyr Ala Ile Tyr Arg Asn Arg Lys Gln Val Thr Val Asp Gln 210 215 220Arg Asn Lys Leu Pro Ala Pro Glu His Val Thr Asp Thr Val Ile Leu225 230 235 240Ser Ile Ala Thr Ser Glu Val Gln Ser Val Asp Ala Lys Gln Cys Tyr 245 250 255Asp Tyr Asp Gly Lys Asp Gly Ser Lys Glu Ser Asp Gly Asp Glu His 260 265 270Glu Lys Cys Val Val Glu Met Val Asp Val Asp Ala Ser Gly Asp Leu 275 280 285Ala Ser Leu 290129257PRTFragaria vesca 129Met Ala Tyr Ser Thr Thr Glu Gln Leu Ala Phe Ser Phe Gly Leu Leu1 5 10 15Gly Asn Ile Val Ser Phe Met Val Phe Leu Ala Pro Met Pro Thr Phe 20 25 30Tyr Arg Ile Tyr Lys Lys Lys Ser Ser Glu Gly Phe Gln Ser Ile Pro 35 40 45Tyr Val Val Ala Leu Leu Ser Ala Met Leu Leu Leu Tyr Tyr Gly Val 50 55 60Ile Lys Thr Asn Ala Ile Leu Ile Ile Ser Ile Asn Ala Phe Gly Ile65 70 75 80Val Ile Glu Val Ala Tyr Leu Ile Phe Tyr Leu Thr Tyr Ala Pro Lys 85 90 95Lys Gln Arg Ile Phe Thr Leu Asn Leu Ile Leu Leu Val Asn Val Ala 100 105 110Phe Gly Leu Thr Leu Ala Ala Thr Ile Phe Leu Leu Ser Gly Thr Lys 115 120 125Arg Val Ala Ala Val Gly Trp Ile Cys Ala Val Phe Asn Ile Ala Val 130 135 140Phe Ala Ala Pro Leu Ser Ile Met Arg Glu Val Ile Arg Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Phe Gly Leu Ser Leu Phe Leu Thr Leu Cys Ala 165 170 175Thr Thr Trp Phe Phe Tyr Gly Leu Phe Thr Lys Asp Tyr Tyr Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Leu Phe Gly Ile Ala Gln Met Ile Leu 195 200 205Tyr Met Val Tyr Arg Asn Ser Gly Lys Asp His Asp Glu Val Glu Ala 210 215 220Lys Thr Arg Thr Asn Gly Asp Asp Leu Glu Met Arg Tyr Lys Val Ile225 230 235 240Ser Lys Ser Lys Thr Thr Asn His Asn Lys Leu Tyr Arg Ser Val Ser 245 250 255Thr130254PRTCicer arietinum 130Met Ser Lys Met Phe Thr Phe Ser Asp His Glu Met Val Leu Ile Phe1 5 10 15Gly Leu Leu Gly Asn Ile Val Ser Phe Leu Val Phe Leu Ala Pro Leu 20 25 30Pro Thr Phe Tyr Ser Ile Cys Lys Lys Lys Thr Ser Glu Gly Phe Gln 35 40 45Ser Ile Pro Tyr Val Val Ala Leu Leu Ser Ala Met Leu Leu Leu Tyr 50 55 60Tyr Gly Leu Leu Lys Thr Asn Ala Ile Leu Ile Ile Thr Ile Asn Cys65 70 75 80Ile Gly Cys Val Ile Glu Val Leu Tyr Leu Ile Ile Tyr Ile Ile Tyr 85 90 95Ala Pro Arg Lys Leu Lys Ile Ser Thr Leu Ala Leu Ile Leu Val Ala 100 105 110Asp Leu Gly Gly Leu Gly Leu Thr Leu Ile Ile Thr Asn Phe Ile Val 115 120 125Lys Ser Tyr Tyr Arg Val His Ala Val Gly Leu Ile Cys Ala Ile Phe 130 135 140Asn Ile Ala Val Phe Ala Ala Pro Leu Ser Ile Met Arg Lys Val Ile145 150 155 160Lys Thr Arg Ser Val Glu Tyr Met Pro Phe Phe Leu Ser Leu Phe Leu 165 170 175Thr Leu Cys Ala Thr Met Trp Phe Phe Tyr Gly Leu Phe Asp Lys Asp 180 185 190Asn Tyr Ile Met Leu Pro Asn Val Leu Gly Phe Leu Phe Gly Ile Ser 195 200 205Gln Met Ile Leu Tyr Leu Ile Tyr Lys Asn Ala Lys Asn Lys Val Glu 210 215 220Ala Asn Ser Asn Glu Gln Gln Glu Tyr Gly Asp Asp Asp Gly Asn Lys225 230 235 240Glu Asp Phe Pro Ser Ile Val Glu Met Lys Glu Asn Ile Val 245 250131255PRTCicer arietinum 131Met Ala Met Thr Arg

Glu Ser Trp Ala Phe Val Phe Gly Leu Leu Gly1 5 10 15Asn Ile Ile Ser Phe Ala Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Leu Ile Phe Lys Lys Lys Ser Thr Glu Gly Phe Gln Ser Leu Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ala Met Leu Trp Ile Tyr Tyr Ala Phe Val 50 55 60Lys Arg Glu Ala Ala Leu Leu Leu Ile Thr Ile Asn Thr Phe Gly Ile65 70 75 80Val Val Glu Ser Cys Tyr Leu Ile Val Phe Leu Ile Tyr Ala Thr Lys 85 90 95Lys Ser Arg Leu Ser Thr Ile Lys Leu Leu Leu Leu Leu Asn Val Phe 100 105 110Gly Phe Gly Ala Met Leu Leu Ser Thr Leu Tyr Leu Ala Lys Gly Ala 115 120 125Lys Arg Leu Ala Ile Ile Gly Trp Ile Cys Leu Val Phe Asn Ile Thr 130 135 140Val Phe Ala Ala Pro Leu Phe Ile Ile Ser Arg Val Ile Arg Thr Arg145 150 155 160Ser Val Glu Tyr Met Pro Phe Phe Leu Ser Phe Phe Leu Thr Ile Asn 165 170 175Ala Val Met Trp Phe Phe Tyr Gly Leu Leu Leu Lys Asp Tyr Tyr Val 180 185 190Ala Leu Pro Asn Thr Leu Gly Phe Val Phe Gly Ile Ile Gln Met Val 195 200 205Met Tyr Leu Ile Tyr Arg Asn Ala Thr Pro Val Pro Leu Asp Gly Pro 210 215 220Val Lys Gly Gln Glu Leu Ser Gly Gly His Ile Val Asp Val Val Lys225 230 235 240Ile Gly Ser Asp Pro Asn Arg Gly Gly Gly Ala Val Ser Lys Val 245 250 255132302PRTArtificial SequenceSynthetic amino acid sequence 132Met Gly Ala Val Gly Ser Pro Trp Ala Phe Leu Phe Gly Val Leu Gly1 5 10 15Asn Val Val Ser Phe Leu Val Tyr Leu Ser Pro Val Pro Thr Phe Tyr 20 25 30Gln Val Phe Lys Lys Lys Ser Thr Gly Gly Phe Ser Ser Ile Pro Tyr 35 40 45Leu Val Ala Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Met Leu 50 55 60Thr Thr Gly Ser Phe Leu Leu Ile Ser Ile Asn Gly Ala Gly Cys Val65 70 75 80Ile Glu Ser Val Tyr Val Val Val Tyr Val Ala Tyr Ala Pro Arg Lys 85 90 95Ala Lys Leu Arg Thr Ala Lys Leu Ile Gly Leu Met Asp Val Gly Gly 100 105 110Phe Gly Ile Val Leu Leu Val Thr His Val Leu Val His Gly Ser Lys 115 120 125Arg Val Gln Ile Val Gly Trp Val Cys Leu Ala Phe Ser Met Cys Val 130 135 140Phe Val Ala Pro Leu Ser Val Ile Arg Arg Val Ile Gln Ser Lys Ser145 150 155 160Val Glu Tyr Met Pro Phe Thr Leu Ser Leu Phe Leu Thr Val Cys Ala 165 170 175Thr Met Trp Leu Ala Tyr Gly Leu Leu Lys Lys Asp Tyr Cys Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Val Phe Gly Ile Ala Gln Met Gly Leu 195 200 205Tyr Val Phe Tyr Arg Asn Arg Lys Pro Val Ile Phe Asp Pro Glu Asp 210 215 220Lys Leu Arg Ala Pro Glu Gln Met Lys Ser Ile Val Ile Leu Ser Thr225 230 235 240Ile Pro Thr Ser Glu Val His Pro Val Asp Ala Lys His Cys Asp Gly 245 250 255Asn Asp Gly Glu Asp Val Asp Gly Lys Asp Gly Asn Lys Glu Gly Asp 260 265 270Gly Asp Glu His Glu Lys Cys Val Val Val Leu Val Asp Met Asp Ala 275 280 285Ser Gly Glu Leu Gln Leu Lys Ser Asp Glu Pro Cys Val Glu 290 295 300133288PRTArtificial SequenceSynthetic amino acid sequence 133Met Ala Met Asn His Ser Thr Leu Ala Phe Val Phe Gly Leu Ile Gly1 5 10 15Asn Ile Val Ser Phe Leu Val Phe Leu Ser Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Cys Lys Ser Lys Ser Ala Glu Gly Tyr His Ser Ile Pro Tyr 35 40 45Val Met Ala Leu Phe Ser Cys Met Leu Trp Ile Phe Tyr Gly Phe Val 50 55 60Thr Ser Gly Asp Phe Leu Leu Ile Thr Ile Asn Ser Val Gly Cys Leu65 70 75 80Ile Glu Ser Val Tyr Val Ile Val Phe Met Ile Tyr Ala Pro Arg Lys 85 90 95Ala Arg Ile Arg Thr Ala Arg Leu Leu Leu Leu Leu Asp Ile Gly Leu 100 105 110Phe Gly Ile Ile Leu Leu Leu Thr Leu Val Leu Thr His Gly Asp Lys 115 120 125Arg Val Val Ile Ile Gly Trp Ile Cys Leu Gly Phe Asn Val Ala Val 130 135 140Phe Ala Ala Pro Leu Ser Val Val Ala Lys Val Val Lys Ser Arg Ser145 150 155 160Val Glu Phe Met Pro Phe Ser Leu Ser Leu Phe Leu Thr Val Cys Ala 165 170 175Val Ala Trp Phe Phe Tyr Gly Phe Leu Leu Lys Asp Tyr Asn Val Ala 180 185 190Leu Pro Asn Ile Ile Gly Leu Val Leu Gly Ile Leu Gln Met Ile Leu 195 200 205Tyr Phe Met Tyr Met Asn Lys Thr Pro Val Ala Ser Gln Val Lys Glu 210 215 220Gly Lys Glu Ala Trp Lys Ala Pro Ala Glu Asp His Val Val Val Ile225 230 235 240Asn Val Gly Lys Ala Asp Lys Ser Ser Cys Ala Glu Val Arg Pro Val 245 250 255Thr Glu Met Ala Gly Ala Val Asp Val Pro Arg Arg Cys Ala Ala Glu 260 265 270Ala Ala Ala Ala Pro Gly Val Asp Phe Ala Arg Ser Val Asn Val Val 275 280 285134333PRTArtificial SequenceSynthetic amino acid sequence 134Met Ala Met Asn His Ser Thr Leu Ala Phe Val Phe Gly Met Ile Gly1 5 10 15Asn Ile Ile Ser Phe Ile Val Phe Leu Ser Pro Leu Pro Thr Phe Tyr 20 25 30Gln Val Phe Lys Lys Lys Thr Ser Glu Gly Tyr His Ser Val Pro Tyr 35 40 45Val Val Ala Leu Phe Ser Ser Met Leu Trp Leu Tyr Tyr Ala Leu Val 50 55 60Lys Ser Gly Ser Phe Leu Leu Ile Thr Ile Asn Ser Phe Gly Cys Val65 70 75 80Val Glu Ser Val Tyr Val Val Met Phe Val Leu Tyr Ala Pro Arg Lys 85 90 95Ala Arg Val Ser Thr Leu Arg Met Ile Leu Leu Leu Val Ile Gly Gly 100 105 110Phe Gly Leu Ile Leu Leu Leu Thr His Leu Leu Ser His Gly Pro Leu 115 120 125Arg Val Gln Val Ile Gly Trp Val Cys Leu Gly Phe Asn Ile Ser Val 130 135 140Phe Val Ala Pro Leu Ser Ile Met Ala Lys Val Ile Gln Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Leu Ser Leu Ser Leu Phe Leu Thr Val Cys Ala 165 170 175Ile Ala Trp Phe Ala Tyr Gly Phe Phe Leu Lys Asp Tyr Asn Ile Ala 180 185 190Leu Pro Asn Val Ile Gly Leu Val Leu Gly Ile Val Gln Met Leu Leu 195 200 205Tyr Met Ile Tyr Arg Asn Lys Lys Pro Ala Ala Ala Ala Val Ile Met 210 215 220Val Glu Glu Val Lys Leu Pro Ala Glu Gln Tyr Ala Ser Lys Glu Val225 230 235 240Ala Pro Pro Ala Ala Ala His Glu Gly Ser Arg Ala Ser Cys Gly Ala 245 250 255Glu Val His Pro Ile Asp Ile Asp Thr Leu Pro Val Ala Asp Val Gly 260 265 270Arg His His Asp Ser Gln Ala Val Val Val Ile Asp Val Asp Val Glu 275 280 285Pro Ala Ala Thr Cys Ala Ala Ala Ala Ala Ala Ala Gly Gly Val Arg 290 295 300Gly Asp Gly Ala Ala Gly Val Val Thr Ala Gly Pro Glu Gln Pro Ala305 310 315 320Ala Met Lys Pro Val Asp Met Ala Ile Ala Val Glu Ala 325 330135296PRTArtificial SequenceSynthetic amino acid sequence 135Met Gly Ala Val Gly Ser Pro Trp Ala Phe Leu Phe Gly Val Ile Gly1 5 10 15Asn Val Val Ser Phe Leu Val Tyr Leu Ala Pro Val Pro Thr Phe Tyr 20 25 30Arg Val Cys Lys Lys Lys Thr Thr Gln Gly Phe His Ser Leu Pro Tyr 35 40 45Ile Met Ala Leu Leu Ser Ala Met Leu Trp Leu Phe Tyr Gly Phe Val 50 55 60Lys Thr Gly Glu Leu Leu Leu Ile Ser Ile Asn Gly Phe Gly Cys Phe65 70 75 80Ile Glu Thr Val Tyr Leu Val Leu Phe Met Ile Tyr Ala Pro Lys Lys 85 90 95Ala Lys Val Ser Thr Leu Arg Ile Ile Gly Leu Leu Asn Phe Gly Val 100 105 110Phe Gly Ile Ile Leu Leu Val Thr His Phe Leu Thr Lys Ala Glu Lys 115 120 125Arg Val Val Ile Leu Gly Trp Val Cys Val Ala Phe Ser Ile Cys Val 130 135 140Phe Ala Ala Pro Leu Ser Val Met Arg Val Val Val Lys Ser Arg Ser145 150 155 160Val Glu Tyr Met Pro Phe Thr Leu Ser Phe Phe Leu Thr Leu Cys Ala 165 170 175Thr Met Trp Phe Phe Tyr Gly Leu Phe Leu Lys Asp Tyr Cys Ile Ala 180 185 190Leu Pro Asn Thr Val Gly Leu Thr Phe Gly Val Ile Gln Met Val Leu 195 200 205Tyr Val Phe Tyr Ser Lys Lys Glu Lys Ala Ile Leu Lys Glu Gln Lys 210 215 220Leu Pro Glu Ile Gln Lys Gly Glu Val Ile Val Lys Asp Glu Asn Met225 230 235 240Asn Ala Asp Lys Lys Phe Pro Glu Leu Thr Gln Glu Gln Ile Ile Asp 245 250 255Ile Val Arg Leu Gly Leu Met Val Cys Lys Gly Lys Val His Val Ala 260 265 270Thr Cys Pro His Gly Thr Thr Cys Glu Pro Lys Val Asp Glu Asn Glu 275 280 285Pro Lys Leu Gln Thr Val Glu Val 290 295136292PRTArtificial SequenceSynthetic amino acid sequence 136Met Gly Ala Val Gly Ser Pro Trp Ala Phe Ile Phe Gly Val Leu Gly1 5 10 15Asn Val Ile Ser Phe Leu Val Phe Leu Ala Pro Leu Pro Thr Phe Tyr 20 25 30Gln Val Tyr Lys Lys Lys Ser Thr Glu Gly Phe Ser Ser Val Pro Tyr 35 40 45Ile Met Ala Leu Leu Ser Cys Met Leu Trp Leu Phe Tyr Ala Leu Leu 50 55 60Thr Thr Asn Ser Leu Leu Leu Ile Thr Ile Asn Ser Ala Gly Cys Leu65 70 75 80Ile Glu Thr Ile Tyr Val Ile Leu Tyr Phe Ile Tyr Ala Pro Lys Lys 85 90 95Ala Lys Ile Phe Thr Ala Lys Met Val Leu Leu Leu Asn Ile Gly Gly 100 105 110Phe Gly Val Val Leu Leu Leu Thr Val Phe Leu Thr Lys Ala Glu Lys 115 120 125Arg Val Gln Ile Ile Gly Trp Ile Cys Val Gly Phe Ala Ile Ala Val 130 135 140Phe Val Ala Pro Leu Ser Val Ile Ala Lys Val Ile Gln Thr Lys Ser145 150 155 160Val Glu Phe Met Pro Leu Thr Leu Ser Phe Phe Leu Thr Val Ser Ala 165 170 175Val Val Trp Phe Leu Tyr Gly Ile Leu Thr Lys Asp Lys Tyr Ile Ala 180 185 190Leu Pro Asn Thr Leu Gly Phe Leu Leu Gly Leu Ala Gln Met Gly Leu 195 200 205Tyr Ala Phe Tyr Lys Lys Arg Glu Thr Ala Met Glu Met Gln Leu Pro 210 215 220Gln His Ser Thr Asp Asn Ile Val Ile Val Ser Ala Ala Thr Asn Ser225 230 235 240Asp Lys Gln Lys Gln His Ser Ser Ser Leu Pro Ser Asn Asn Leu Val 245 250 255Gly Ala Ala Val Asp Asp Asp Asp Val Thr Thr Thr Thr Lys Asn Gly 260 265 270Ile Asp Pro Ile Asn Asn Leu Glu Gln Asn His Gln Val Lys Asp Gln 275 280 285Leu Asn His Val 290137287PRTArtificial SequenceSynthetic amino acid sequence 137Met Gly Ala Val Gly Ser Pro Trp Ala Phe Leu Phe Gly Val Leu Gly1 5 10 15Asn Val Val Ser Phe Leu Val Tyr Leu Ser Pro Val Pro Thr Phe Tyr 20 25 30Gln Val Phe Lys Lys Lys Ser Thr Gly Gly Phe Ser Ser Ile Pro Tyr 35 40 45Leu Val Ala Leu Leu Ser Ala Met Leu Trp Leu Tyr Tyr Ala Met Leu 50 55 60Thr Thr Gly Ser Phe Leu Leu Ile Ser Ile Asn Gly Ala Gly Cys Val65 70 75 80Ile Glu Ser Val Tyr Val Val Val Tyr Val Ala Tyr Ala Pro Arg Lys 85 90 95Ala Lys Leu Arg Thr Ala Lys Leu Ile Gly Leu Met Asp Val Gly Gly 100 105 110Phe Gly Ile Val Leu Leu Val Thr His Val Leu Val His Gly Ser Lys 115 120 125Arg Val Gln Ile Val Gly Trp Val Cys Leu Ala Phe Ser Met Cys Val 130 135 140Phe Val Ala Pro Leu Ser Val Ile Arg Arg Val Ile Gln Ser Lys Ser145 150 155 160Val Glu Tyr Met Pro Phe Thr Leu Ser Leu Phe Leu Thr Val Cys Ala 165 170 175Thr Met Trp Leu Ala Tyr Gly Leu Leu Lys Lys Asp Tyr Cys Ile Ala 180 185 190Leu Pro Asn Val Leu Gly Phe Val Phe Gly Ile Ala Gln Met Gly Leu 195 200 205Tyr Val Phe Tyr Lys Tyr Cys Lys Thr Ser Pro His Leu Gly Glu Lys 210 215 220Glu Val Glu Ala Ala Lys Leu Pro Glu Val Ser Leu Asp Met Leu Lys225 230 235 240Leu Gly Thr Val Ser Ser Pro Glu Pro Ile Ser Val Val Arg Gln Ala 245 250 255Asn Lys Cys Thr Cys Gly Asn Asp Arg Arg Ala Glu Ile Glu Asp Gly 260 265 270Gln Thr Pro Lys His Gly Lys Gln Ser Ser Ser Ala Ala Ala Thr 275 280 28513836DNAArtificial SequenceSynthetic DNA 138taatgtcgac atgaacatcg ctcacactat cttcgg 3613939DNAArtificial SequenceSynthetic DNA 139tatgagctct taaacttgaa ggtcttgctt tccattaac 3914036DNAArtificial SequenceSynthetic DNA 140taatgtcgac atggatgttt ttgctttcaa tgcttc 3614136DNAArtificial SequenceSynthetic DNA 141tatgagctct cacacgtaag aaacaatcaa aggctc 3614237DNAArtificial SequenceSynthetic DNA 142taatgtcgac atgggtgata aacttcgatt atccatc 3714337DNAArtificial SequenceSynthetic DNA 143tatgagctct tagatcgatg aggcattgtt agaattc 3714441DNAArtificial SequenceSynthetic DNA 144taatgtcgac atggttaacg ctacagttgc gagaaacatt g 4114538DNAArtificial SequenceSynthetic DNA 145tatgagctct caagctgaaa ctcgtttagc ttgtccac 3814640DNAArtificial SequenceSynthetic DNA 146taatgtcgac atgacggacc cccacaccgc ccggacgatc 4014740DNAArtificial SequenceSynthetic DNA 147tatgagctct caagcctggc caagttcgat tccagcattc 4014843DNAArtificial SequenceSynthetic DNA 148taatgtcgac atggtgcatg aacagttgaa tcttattcgg aag 4314941DNAArtificial SequenceSynthetic DNA 149tatgagctct caaacgccgc taactctttt gtttaaatat g 4115038DNAArtificial SequenceSynthetic DNA 150taatgtcgac atggtgtttg cacatttgaa ccttcttc 3815140DNAArtificial SequenceSynthetic DNA 151tatgagctct taaacattgt taggttcttg gttggtattc 4015241DNAArtificial SequenceSynthetic DNA 152taatgtcgac atgttcctca aggttcatga aattgctttt c 4115336DNAArtificial SequenceSynthetic DNA 153tatgagctct cacttcattg gcctcaccga tccttc 3615439DNAArtificial SequenceSynthetic DNA 154taatgtcgac atgagtctct tcaacactga aaacacatg 3915536DNAArtificial SequenceSynthetic DNA 155tatgagctct catgtagctg ctgcggaaga ggactg 3615639DNAArtificial SequenceSynthetic DNA 156taatgtcgac atggctctct tcgacactca taacacatg 3915738DNAArtificial SequenceSynthetic DNA 157tatgagctct caagtagttg cagcactgtt tctaactc 3815841DNAArtificial SequenceSynthetic DNA 158ggaattccat atggctctaa ctaacaattt atgggcattt g 4115941DNAArtificial SequenceSynthetic DNA 159taatgtcgac ttaaacttga ctttgtttct ggacatcctt g 4116040DNAArtificial SequenceSynthetic DNA 160taatgtcgac atgggagtca tgatcaatca ccatttcctc 4016136DNAArtificial

SequenceSynthetic DNA 161tatgagctct caaacggttt caggacgagt agcctc 3616239DNAArtificial SequenceSynthetic DNA 162taatgtcgac atggcagagg caagtttcta tatcggagt 3916338DNAArtificial SequenceSynthetic DNA 163tatgagctct taagagagga gaggttcaac acgtgatg 3816420DNAArtificial SequenceSynthetic DNA 164gtaaaacgac cagtcttaag 2016517DNAArtificial SequenceSynthetic DNA 165caggaaacag ctatgac 1716621DNAArtificial SequenceSynthetic DNA 166tgctgtactt gcttggtatt g 2116718DNAArtificial SequenceSynthetic DNA 167ggaccagacc agacaacc 18168909DNAArtificial SequenceSynthetic DNA 168atgggtgctg ttggttctcc ttgggctttc ctttttggtg tgttgggtaa tgttgtttct 60ttcttggttt atttgtctcc tgttcctact ttctaccaag ttttcaagaa aaagagtaca 120ggaggttttt cttcaattcc ttacttggtt gctcttttgt ctgcaatgct ttggttgtat 180tacgctatgc ttactacagg atcattcctc ctcatatcta ttaacggagc aggttgtgtg 240atcgaatcag tttatgttgt ggtttatgtt gcttacgcac caagaaaggc taagttgagg 300accgcaaaat tgattggact catggatgtt ggaggttttg gtatcgttct tttggtgaca 360catgttcttg tgcacggatc aaagagagtt caaattgtgg gttgggtttg tttggctttt 420tctatgtgcg ttttcgtggc acctctctca gtgatcagaa gggttataca gagtaaatct 480gtggagtaca tgccttttac tctctcttta ttccttacag tttgtgctac catgtggttg 540gcttatggac tcttaaaaaa ggattactgc atagctttac ctaatgtgct tggatttgtt 600ttcggtattg cacaaatggg tctttatgtg ttttacagaa acaggaagcc tgttatcttc 660gatccagaag ataaattaag agcacctgag cagatgaagt ctatagttat tctctcaacc 720atacctacta gtgaagtgca tccagttgat gctaaacact gtgatggaaa tgatggtgaa 780gatgttgatg gaaaagatgg taacaaggag ggagatggag atgaacatga gaagtgcgtg 840gttgtgctcg tggatatgga tgctagtgga gagttacagt tgaagtctga tgaaccttgt 900gtggaatga 909169867DNAArtificial SequenceSynthetic DNA 169atggctatga accactctac tctcgctttt gttttcggat tgatcggtaa tatcgtttct 60ttcttagttt tcctcagtcc tgtgcctact ttctacagag tttgtaagag taaatctgct 120gaaggttatc attcaatacc ttacgttatg gctcttttta gttgcatgtt gtggatcttt 180tacggattcg ttacctctgg agattttctt ttgataacta tcaatagtgt gggatgtttg 240attgagtctg tgtatgttat tgtgtttatg atctatgctc caagaaaggc aagaattagg 300acagctaggc tcttactttt gctcgatatc ggacttttcg gtattatcct ccttttgact 360cttgttttga cacacggaga taaaagagtt gtgataattg gatggatctg tctcggtttt 420aacgttgctg tgttcgctgc acctctttct gttgtggcaa aggttgtgaa atcaaggagt 480gttgaattca tgccattctc tctctcatta tttcttaccg tttgcgctgt ggcatggttt 540ttctatggat tcctcttaaa ggattacaat gttgctttgc ctaacatcat aggattggtg 600ctcggtatcc tccaaatgat actctacttc atgtacatga ataagacacc tgttgcttca 660caggtgaagg aaggaaaaga ggcatggaaa gctccagcag aggatcatgt tgtggttatt 720aacgttggaa aggctgataa atcttcatgc gcagaagtta gacctgtgac cgagatggct 780ggagcagttg atgtgccaag aaggtgtgca gcagaagcag cagcagcacc tggagtggat 840ttcgcaagga gtgtgaatgt tgtttga 8671701002DNAArtificial SequenceSynthetic DNA 170atggctatga accactctac tctcgctttt gtgtttggta tgatcggaaa tattatctct 60tttatcgtgt ttttgtctcc actccctact ttctaccaag tgttcaagaa aaagacaagt 120gaaggatatc attctgttcc ttacgttgtg gctcttttct cttcaatgct ttggttgtat 180tacgctttgg ttaaaagtgg atcttttctt ttgattacca tcaattcatt cggttgtgtt 240gtggagagtg tgtatgttgt gatgtttgtt ctctacgctc ctagaaaggc aagggtgagt 300acattgagaa tgatcctctt acttgttata ggaggtttcg gacttatctt gctcttaacc 360catcttttgt cacacggtcc actcagggtg caagttatag gatgggtttg tttaggtttt 420aacatttctg tgttcgttgc tcctttgtca attatggcaa aagtgatcca gaccaagtct 480gttgaattca tgccactctc attaagtctt ttcttgactg tttgcgctat tgcatggttc 540gcttatggat ttttccttaa ggattacaac atagctttgc ctaacgttat tggactcgtg 600ttaggtatcg ttcaaatgct cctctacatg atctacagaa ataagaagcc tgctgcagct 660gcagtgataa tggttgaaga ggtgaaactt ccagctgaac agtacgcatc taaggaggtt 720gctcctccag ctgcagctca tgaaggatct agagcttcat gtggtgcaga ggttcaccct 780atagatattg atacattgcc agttgctgat gtgggaaggc atcacgattc acaggctgtt 840gtggttatcg atgtggatgt tgagccagca gctacttgcg ctgcagcagc tgcagctgca 900ggaggtgtta gaggagatgg tgctgctggt gtggttacag ctggtcctga acaacctgct 960gctatgaaac ctgtggatat ggctattgct gtggaggcat ga 1002171891DNAArtificial SequenceSynthetic DNA 171atgggtgctg tgggtagtcc ttgggctttt ctcttcggtg ttattggtaa tgttgtgtct 60ttcctcgtgt atctcgctcc tgttccaact ttctacagag tttgtaagaa aaagactaca 120caaggattcc atagtttgcc ttatattatg gctcttttgt ctgcaatgtt gtggctcttt 180tacggattcg ttaagaccgg tgaacttctc cttatatcta ttaacggatt tggttgcttc 240atcgaaactg tgtatcttgt tctttttatg atatacgctc caaaaaaggc aaaagtttca 300actcttagaa ttatcggatt gctcaacttt ggagtgttcg gtataattct ccttgttacc 360cacttcttaa ctaaagctga gaagagggtt gtgattcttg gttgggtgtg tgttgcattt 420tcaatctgcg tgttcgctgc acctttgagt gttatgagag ttgtggttaa atctagatca 480gttgaataca tgccttttac tctctctttc tttcttacac tctgtgctac catgtggttt 540ttctatggat tattccttaa ggattactgc atagcattgc ctaatactgt gggactcaca 600tttggtgtta tccagatggt gttgtatgtt ttctactcaa aaaaggagaa ggctattctc 660aaggaacaaa agcttcctga gatacagaaa ggtgaagtga ttgttaagga tgaaaacatg 720aacgctgata agaagttccc agaacttaca caagagcaga tcatagatat cgtgaggttg 780ggactcatgg tttgtaaagg aaaggtgcat gttgctacct gcccacacgg aaccacctgt 840gaacctaaag ttgatgagaa cgaaccaaag ttgcagactg ttgaagtgtg a 891172879DNAArtificial SequenceSynthetic DNA 172atgggtgctg ttggtagtcc ttgggctttt atctttggtg tgttgggtaa tgtgatttct 60ttcttggtgt tccttgctcc tcttcctaca ttctaccaag tttacaagaa aaagtcaacc 120gaaggattct cttcagtgcc ttatatcatg gctcttttga gttgtatgtt atggcttttc 180tatgcactcc tcactacaaa ctctcttttg ctcataacta ttaactcagc tggttgcctt 240attgagacaa tctatgttat tttgtacttc atctacgctc caaaaaaggc aaagattttc 300actgctaaga tggttctcct tttgaacatt ggaggtttcg gagttgtgct cttacttact 360gttttcttga caaaagctga aaagagagtt caaattatcg gatggatatg tgtgggtttt 420gctattgcag ttttcgtggc tcctctcagt gttattgcaa aagtgatcca gacaaagtct 480gttgagttta tgccattgac cctctcattt ttccttactg tgagtgctgt tgtgtggttc 540ttatatggaa tccttaccaa ggataagtac atagcattgc ctaatacact cggatttttg 600ctcggtttag ctcagatggg tctttatgca ttctacaaaa agagggaaac tgctatggag 660atgcaacttc ctcagcattc aactgataat atcgttatag tgagtgctgc aacaaactct 720gataaacaaa agcagcacag ttcttcattg ccatctaata acctcgttgg agctgcagtg 780gatgatgatg atgttaccac tacaaccaaa aacggtatag atccaatcaa caacctcgaa 840cagaatcatc aagtgaaaga tcagttaaac cacgtttga 879173864DNAArtificial SequenceSynthetic DNA 173atgggtgctg tgggttctcc ttgggctttt ctctttggtg ttctcggtaa tgtggtttct 60tttcttgtgt atttgagtcc tgttccaaca ttctaccaag ttttcaagaa aaagtcaacc 120ggaggttttt cttcaatacc ttacttggtt gctcttttga gtgcaatgct ttggttgtat 180tacgctatgc tcactacagg atctttcctc ctcatatcta ttaacggagc tggttgtgtt 240atcgagtctg tgtatgttgt ggtttatgtt gcttacgcac caagaaaggc taagttgagg 300accgcaaaat taatcggact tatggatgtt ggaggttttg gtatagtgct tttggttaca 360catgtgcttg ttcacggtag taagagagtg caaattgttg gttgggtgtg tttggctttt 420agtatgtgcg tgttcgttgc acctctctct gttataagaa gagttattca gtcaaaaagt 480gttgaataca tgccttttac tctctcatta ttccttaccg tgtgtgctac tatgtggttg 540gcttatggac tcttaaaaaa ggattactgc attgctttgc ctaacgttct cggatttgtg 600ttcggtatcg ctcaaatggg actttatgtt ttctataagt actgcaagac atctcctcat 660cttggtgaaa aagaggttga agctgcaaag ttaccagagg tgtctcttga tatgttgaaa 720ctcggaactg ttagttctcc tgaaccaatc tcagtggtta gacaggctaa taagtgtaca 780tgcggtaacg atagaagggc agagatagaa gatggtcaaa cacctaagca cggaaagcag 840tcaagttcag cagcagcaac atga 864174909DNAArtificial SequenceSynthetic DNA 174atgggagcag tcggttcgcc gtgggccttt ttgttcggag ttcttggtaa tgtggtctcg 60tttctcgtgt atctctcacc cgttcctacg ttctaccagg tgttcaagaa gaagtccact 120ggcgggttct ccagcatccc gtatctggtg gctctcctgt ctgcaatgct ctggctgtac 180tatgccatgc ttaccacagg atcgttcctt ttgatcagta ttaacggcgc ggggtgcgtt 240attgagtcag tctacgtggt cgtttacgtc gcctatgcac caagaaaggc aaagctcagg 300accgcaaagc tgatcggact tatggacgtg ggaggtttcg gtattgtcct cctggttaca 360cacgtgctcg tccatggcag caagcgggtg cagatcgtcg ggtgggtttg cctggccttc 420tcaatgtgtg tttttgtggc gccgctttcg gtcatccgca gggttattca atctaagtca 480gtcgaataca tgccattcac cctttccttg tttctcaccg tttgcgctac aatgtggctg 540gcatacggcc ttttgaagaa ggattattgt atcgctttgc ctaacgtgct cggcttcgtc 600tttgggattg cacagatggg cctctacgtt ttctatcgga atagaaagcc ggtcatcttt 660gacccagagg ataagttgag ggctcccgaa cagatgaagt ccatcgtcat tctcagcacg 720attcctactt ctgaggtgca ccccgtcgac gcgaagcatt gcgacggcaa cgatggggag 780gacgtggatg gaaaggatgg taataaggaa ggagacggcg acgagcacga aaagtgtgtg 840gtcgttttgg tggatatgga tgccagcggg gaattgcagt tgaagagtga tgaaccctgt 900gtggagtga 909175879DNAArtificial SequenceSynthetic DNA 175atgggggcag tgggttcgcc ttgggccttt atttttgggg tcctcggcaa tgtgatttcc 60tttctggttt tcctggctcc tcttcctacg ttctaccagg tttacaagaa gaagtccact 120gagggctttt ccagcgtgcc gtacattatg gccctcctgt cctgcatgtt gtggctcttc 180tatgcgcttt tgaccacaaa ctcactcctg cttatcacca ttaattcggc cggatgtctc 240atcgaaacaa tctacgtgat cctgtacttc atctacgctc caaagaaggc aaagatcttt 300acggcgaaga tggtgttgct cctgaacatc ggcgggttcg gcgtggtcct tttgctcacg 360gtctttctga ctaaggctga gaagcgcgtg cagatcattg gctggatttg cgtcgggttc 420gccatcgcgg tctttgttgc cccgctcagt gttattgcga aggtcatcca aacaaagtct 480gtcgaattca tgccactgac ccttagcttc tttctcacag tttctgctgt tgtgtggttc 540ttgtacggca ttctcacgaa ggacaagtat atcgcactgc cgaacactct tggattcctg 600cttggtttgg ctcaaatggg gctctacgca ttttataaga agagggagac cgcgatggaa 660atgcagctcc ctcaacactc aaccgacaac atcgtgattg tcagtgccgc gacaaattcc 720gataagcaga agcaacattc ttcatcgctg cccagcaaca atcttgtcgg agctgcagtt 780gacgatgacg atgtcacgac taccacaaag aatggtatcg acccaatcaa taacctggag 840caaaaccatc aagtgaagga ccaactgaat catgtgtga 879

Claims

1. A method for producing an exudate, comprising the steps of cultivating a transformed plant in which a nucleic acid encoding a transporter protein having a consensus sequence comprising the following amino acid sequence: (L/I/V/M/F)x(G/A)xx(I/L/V/M/F)xxxx(L/I/V/F)(A/S)(P/S) [SEQ ID NO: 1] (1-3aa) (P/S/T/A)T (F/L)xx(I/V)xxxKxxxxxxxxPYxxx(L/I)xxxx(L/I)x(I/L/M/V/F)xY(A/S/G) [SEQ ID NO: 2] (7-13aa)(I/L/V/M)(1-2aa)(I/V)Nxxxxxx(E/Q)xxYxxx(Y/F)xx(Y/F)(A/G/S) [SEQ ID NO: 3] (35-36aa)(R/Q/H)xxxxGx(V/I/L)xxxxx(V/M/L/I/F)xxxx(A/S/T)P(L/M)x(I/V)(I/M/- V/L) [SEQ ID NO: 4] (2-7aa) (V/I) (V/I/M)x(T/S)x(S/N)xx(F/Y) (M/L) (P/S) (F/I/V/L)xLSxx(L/I) (T/V)xx(A/G)xx W(F/L)xYGxxxxDxx(V/I)xxPNxxGxx(F/L) (G/S)xxQ(M/I)x(L/M/I/V/F) (Y/H/F) [SEQ ID NO: 5] and being involved in sugar transportation is introduced and/or expression of the protein is enhanced; and collecting an exudate from the transformed plant.

2. The method for producing an exudate according to claim 1, wherein the transformed plant is cultivated under conditions at a relative humidity of 80% RH or more.

3. The method for producing an exudate according to claim 1, wherein the exudate is guttation.

4. The method for producing an exudate according to claim 1, wherein the transporter protein is a protein in the clade III among the clades I to V of taxonomic groups based on the amino acid sequences of the SWEET proteins.

5. The method for producing an exudate according to claim 1, wherein the transporter protein is a protein of any of the following (a) and (b): (a) a protein comprising any of the amino acid sequences set forth in SEQ ID NOs: 15 to 137; (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any one of SEQ ID NOs: 15 to 137 and having transporter activity involved in sugar transportation.

6. The method for producing an exudate according to claim 1 wherein the consensus sequence comprises the following amino acid sequence: TABLE-US-00030 [SEQ ID NO: 6] G(L/I/V/F/M)xGx(I/V/L)(I/V/L)(S/T)xxxxL(A/S)P(L/ V/I/M)(P/S/T/A)TFxx(I/V)x(K/R)xK(S/T)xxx(F/Y)x(S/ A)xPYxx(A/S/T)LxSxxLx(L/I/M/V)(Y/F)Y(A/G) [SEQ ID NO: 7] (7-9 aa)(L/I)(I/V/L)(T/S)INxx(G/A)xx(I/V/M)(E/Q) xxYxxx(F/Y)(L/I/V/F)x(Y/F)Ax(K/R/N)xxxxx(T/A) [SEQ ID NO: 8] (7-8 aa)(V/F/L/I/M)(18-19 aa)(R/Q/H)xxxxGx(I/V) xxxxx(V/I/L/M)x(V/M)F(A/V)(A/S/T)PLx(I/V)(I/M/V/L) xxV(I/V)(K/R/Q)(T/S)(K/R)S(V/A)x(F/Y)MP(F/I/L)xLS (L/F/V)xL(T/V)(L/I)xAxxW(F/L)xYG(L/F)xxxDxx(V/I) xxPNxxGxx(L/F)(G/S)xxQMx(L/V/I)(Y/F)xx(Y/F).

7. The method for producing an exudate according to claim 6, wherein the transporter protein is a protein of any of the following (a) and (b): (a) a protein comprising any of the amino acid sequences set forth in SEQ ID NOs: 15 to 35; (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any one of SEQ ID NOs: 15 to 35 and having transporter activity involved in sugar transportation.

8. The method for producing an exudate according to claim 1, wherein the consensus sequence comprises the following amino acid sequence: TABLE-US-00031 [SEQ ID NO: 9] (A/V)xxxG(I/L/V)xGN(I/L/V)(I/L/V)S(F/L)x(V/T)xL (A/S)P(V/L/I)(P/A)TFxx(I/V)x(K/R)xK(S/T)xx(G/S) (F/Y)(Q/S/E)SxPYxx(A/S/T)LxS(A/C/S)xLx(L/I/M)(Y/F) Y(A/G)xx(K/T) [SEQ ID NO: 10] (3-5 aa)(L/M/P)(L/I)(I/L/V)(T/S)INxx(G/A)xx(I/V) (E/Q)xxY(I/L)x(L/M/V/I)(F/Y)(L/I/V/F)x(Y/F)Ax(K/R) xxxxx(T/A)xx(L/M/F/V/I)(L/F/V/I)xxx(N/D)(F/V/I/L) xx(F/L)xx(I/L/V)xxxxxx(L/I/V) [SEQ ID NO: 11] (5-6 aa)(R/Q)xxxxGx(I/V)xxxx(S/A)(V/L/M)(C/S/A)VF (A/V)(A/S)P Lx(I/V)(I/M/V)xxV(I/V)(K/R/Q)(T/S)(K/ R)S(V/A)E(F/Y)MP(F/I)xLS(L/F/V)xL(T/V)(L/I)(S/N)A (V/I)xW(F/L)xYGLxx(K/N)Dxx(V/I)xxPN(V/I)xGxx(F/L) (G/S)xxQMxL(Y/F)xx(Y/F).

9. The method for producing an exudate according to claim 8, wherein the transporter protein is a protein of any of the following (a) and (b): (a) a protein comprising any of the amino acid sequences set forth in SEQ ID NOs: 15 to 26; (b) a protein having an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any one of SEQ ID NOs: 15 to 26 and having transporter activity involved in sugar transportation.

10. The method for producing an exudate according to claim 1, wherein the consensus sequence comprises the following amino acid sequence: TABLE-US-00032 [SEQ ID NO: 12] (M/L/V)xx(T/K/N/S)xxxxAxxFG(L/I/V)LGN(I/L/V)(I/V) SFxVxL(S/A)P(V/I)PTFxxIxK(K/R)K(S/T)x(E/K)(G/S)(F/ Y)(Q/E)S(I/L)PYxx(A/S)LxS(A/C)xLx(L/I/M)YY(A/G)xxK [SEQ ID NO: 13] (4-5 aa)(L/M)(L/I)(I/V)(T/S)IN(A/S/T)(F/V)(G/A)x (F/V)(I/V)(E/Q)xxY(I/L)x(L/M/I)(F/Y)(F/V/I/L)x(Y/ F)Ax(K/R)xx(R/K)xx(T/A)(L/V/M)K(V/L/M/F)(L/I/V/F) xxx(N/D)(F/V/I)xx(F/L)xx(I/L)(L/I/V/F)(L/M/V)(L/V) xx(F/L)(L/I/V) [SEQ ID NO: 14] (5-6 aa)(R/Q)x(K/S/Q)x(L/I/V)Gx(I/V)Cxxx(S/A)(V/L) (S/C/A)VF(A/V)(A/S)PLx(I/V)(M/I/V)xxV(I/V)(K/R)T (K/R)S(V/A)E(Y/F)MPFxLS(L/F)xLT(I/L)(S/N)A(V/I)xW (L/F)xYGLx(L/I)(K/N)Dxx(V/I)A(L/F/I/M)PN(V/I)(L/ I/V)Gxx(L/F)GxxQM(I/V)L(Y/F)(V/L/I/M)(V/L/I/M)(Y/ F)(K/R/Q).

11. The method for producing an exudate according to claim 10, wherein the transporter protein is a protein of any of the following (a) and (b): (a) a protein comprising any of the amino acid sequences set forth in SEQ ID NOs: 15 to 21; (b) a protein comprising an amino acid sequence having an identity of 90% or more to an amino acid sequence set forth in any one of SEQ ID NOs: 15 to 21.

12. The method for producing an exudate according to claim 1, wherein the transformed plant is a phanerogam.

13. The method for producing an exudate according to claim 12, wherein the phanerogam is an angiosperm.

14. The method for producing an exudate according to claim 13, wherein the angiosperm is a monocot.

15. The method for producing an exudate according to claim 14, wherein the monocot is a plant of the family Poaceae.

16. The method for producing an exudate according to claim 15, wherein the plant of the family Poaceae is a plant of the genus Oryza.

17. The method for producing an exudate according to claim 13, wherein the angiosperm is a dicot.

18. The method for producing an exudate according to claim 17, wherein the dicot is a plant of the family Brassicaceae.

19. The method for producing an exudate according to claim 18, wherein the plant of the family Brassicaceae is a plant of the genus Arabidopsis.