METHODS FOR DETERMINING THE EFFICACY PROFILE OF A DRUG CANDIDATE

Abstract

This patent application relates to a method for determining the in vitro efficacy profile of a drug candidate using standardized cell cultures of uniformly distributed differentiated neural cells (NCs) from at least two primate species, wherein the differentiated NC cultures are qualified for high throughput screening based on a dissociation and reseeding step performed on the differentiated NCs. The method includes differentiating human and/or non-human primate neuronal precursor cells (NPCs) to neuronal cells (NCs) followed by dissociating the differentiated NCs from its support and reseeding the differentiated NCs in a high-throughput cell culture format resulting in robust cultures suitable for high-throughput drug screening assays, in particular to screen antisense oligonucleotides.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of PCT International Application No. PCT/EP2016/077435, filed on Nov. 11, 2016, the entire contents of which are incorporated herein in by reference, and which claims priority to European Patent Application No. 16189502.4 filed on Sep. 19, 2016 and European Patent Application No. 15194367.7 filed on Nov. 12, 2015.

SEQUENCE LISTING

[0002] This application contains a Sequence Listing submitted via EFS-Web and hereby incorporated by reference in its entirety. Said ASCII copy, created on Apr. 20, 2018, is named P33890-US_SeqListing.txt, and is 554,456 bytes in size.

FIELD OF THE INVENTION

[0003] This patent application relates to a method for determining the in vitro efficacy profile of a drug candidate using standardized cell cultures of uniformly distributed differentiated neural cells (NCs) from at least two primate species, wherein the differentiated NC cultures are qualified for high throughput screening based on a dissociation and reseeding step performed on the differentiated NCs. The method includes differentiating human and/or non-human primate neuronal precursor cells (NPCs) to neuronal cells (NCs) followed by dissociating the differentiated NCs from its support and reseeding the differentiated NCs in a high-throughput cell culture format resulting in robust cultures suitable for high-throughput drug screening assays, in particular to screen antisense oligonucleotides.

BACKGROUND OF THE INVENTION

[0004] High-throughput screening assays based on diverse cell types or cell culture models displaying the pathophysiology of various diseases, including diseases of the central nervous system (CNS), are widely employed to assess efficacy and toxicity profiles of drug candidates early in development. Restrictions to limited types of cells or to cells derived from embryonic stem cell have been overcome in the past decade by establishing protocols to generate pluripotent cell from somatic cells. Since Yamanaka and colleagues (Takahashi, K. & Yamanaka, S. Cell. 2006; 126:663-676) demonstrated that somatic cells can be reprogrammed to induced pluripotent stem cells (IPSCs) it became possible to generate pluripotent cells from a variety of cell sources. Different types of somatic cells including fibroblasts, keratinocytes, adipocytes and blood cells have been reprogrammed to an IPSC pluripotent state. More recently, specific somatic cell types could be transdifferentiated to a completely different somatic cell type such as a neuron. Vierbuchen and colleagues demonstrated the direct conversion of mouse fibroblasts to functional neurons by transduction of three crucial genes: Mash1, Brn2 and Myt1I (Vierbuchen et al. Nature. 2010; 463:1035-41). Notably, US2010/0021437 discloses a method for generating induced pluripotent stem cells from fibroblasts and inducing those cells to differentiate into NPCs. More recently direct conversion of differentiated somatic cells to NPCs has been described (WO2012/022725). Such neuronal cells are thought to be a valuable tool for modelling the pathophysiology of various CNS diseases.

[0005] NPCs are multipotent stem cells and propagate under specific conditions. They can grow as a monolayer adherent culture or as floating neurospheres in non-adherent cell culture plates. The two types of NPC cultures (neurospheres, adherent cultures) seem to be completely inter-convertible. NPCs can be grown indefinitely and still remain truly multipotent. Upon special conditions they differentiate into the neuronal cell types that compose the adult brain, including differentiated NCs. Differentiated NC cultures are a valuable disease model to screen effective and safe drugs. Indeed, NC cultures are important to assess toxicity and efficacy of drug candidates in a drug development setting.

[0006] Before administered in human patients for the first time, drug candidates need to be evaluated thoroughly in in vitro cell culture systems followed by in vivo testing in rodent and non-human primate (NHP) species. Today, the toxicity and efficacy assessment of novel drug candidates is performed in different assay formats using different protocols for different species. While the in vitro testing of drug candidates allows to reduce the number of laboratory animals sacrificed for drug testing, it also posed challenges of outcome translatability from in vitro to in vivo and to the human physiology. Indeed, the translatability of the efficacy and toxicity profiles of drug candidates from rodent species to humans can be challenging, due to non-close genetic relation between the species. Notably, the protein-coding regions of the mouse and human genome are about 85% identical. The difference in genetic makeup may account for different physiological reactions to drugs and also to different tolerance against toxins or mutagens. Translatability between species becomes even more important to date with novel drug classes targeting specific DNA or RNA sequences, as e.g. antisense oligonucleotides, or genetic variants of a gene or gene product. Therefore, in view of the close genetic relatedness (>90%), NHP species represent the most meaningful model system to generate efficacy and toxicity data to predict response and adverse event rate in humans. Without being bound to theory it is assumed that in vivo tests in NHP species, e.g. cynomolgus monkey, are preferable prior to first human trials for all drug classes, but especially for drug classes targeting a defined human genetic makeup. On the other hand, the use of NHP species in drug discovery remains a controversial matter. The very fact that NHP species are genetically close to humans raises ethical questions. Therefore, the number of NHP laboratory animals, e.g. cynomolgus monkey, should be reduced as much as possible.

[0007] A comprehensible setup for efficacy and toxicity screening of a novel drug candidate should, therefore, comprise both in vitro and in vivo tests on NHP species, and parallel in vitro tests using IPSC derived human cells. The sequence of testing should include parallel in vitro tests both on a NHP species and human cells preceding in vivo tests in the NHP species, e.g. cynomolgus monkey. Drug candidates with poor efficacy and/or toxicity profile should already be rejected at an early stage upon in vitro assessment on NHP and/or human cells before starting in vivo tests in an NHP species. Indeed, this sequence ensures that the number of NHP laboratory animals can be kept as low as possible.

[0008] However, there are formidable hurdles for gaining such primate inter-species transferable efficacy and toxicity data in vitro from stem cell-derived differentiated NCs: first, species specific cell culture protocols and non-transferability of cell culture conditions between primate species and, second, inhomogeneous distribution of the differentiating NPCs leading to non-optimal survival conditions or hampered differentiation effects due to local concentration of cells and autocrine and paracrine signalling leading to challenges in phenotypic assessment of drug effects. This becomes most pronounced when the cultures have to be differentiated for a long period of time to obtain the desired differentiation state of the cells. Most notably, differentiated primate NCs are innate sensitive to cell culture conditions and do not tolerate harsh treatment which is an inherent obstacle to producing standardized assays with these cells.

[0009] Hence, there remains a need for an easy accessible and reproducible technology for the generation of uniform NC assays from different primate species including humans for high-throughput screening of drug candidates.

SUMMARY OF THE INVENTION

[0010] Provided herein is a method for determining the in vitro efficacy profile of a drug candidate using standardized cell cultures of uniformly distributed differentiated neural cells (NCs) from at least two primate species, wherein the differentiated NC cultures are qualified for high throughput screening, the method comprising the steps of:

[0011] a) dissociating the differentiated NCs from its support after about 20 days to about 45 days of differentiation and reseeding the differentiated NCs in a high-throughput cell culture format;

[0012] b) incubating the reseeded NCs in a differentiation medium;

[0013] c) contacting the reseeded NCs with the drug candidate; and

[0014] d) determining the in vitro efficacy profile of the drug candidate.

[0015] In one embodiment, the primate species are selected from the group consisting of human (Homo sapiens), Cynomolgus monkey (Macaca fascicularis) and Rhesus monkey (Macaca mulatta).

[0016] In one embodiment, one of the primate species is human (Homo sapiens).

[0017] In one embodiment, one of the primate species is Cynomolgus monkey (Macaca fascicularis).

[0018] In one embodiment, the differentiated NCs are derived from induced pluripotent stem cells (iPSCs).

[0019] In one embodiment, the differentiated NCs are uniformly distributed over the cell culture area as assessed by cell nucleus staining.

[0020] In one embodiment, the distribution of the differentiated NCs is assessed by DNA staining, in particular by Hoechst staining.

[0021] In one embodiment, step d) additionally comprises monitoring the cell cultures for signs of toxicity.

[0022] In one embodiment, step d) comprises monitoring the cell cultures for a phenotypic change indicative of the efficacy of the drug candidate.

[0023] In one embodiment, the determined in vitro efficacy profile of the drug candidate is used for inter-species comparison of the efficacy profile of a drug candidate, wherein the cell cultures are produced individually from cells of at least two primate species, wherein essentially the same conditions are applied to the cultures for all primate species and wherein the efficacy profile is determined and compared for all primate species.

[0024] In one embodiment, provided is a method for selecting a drug candidate for further development comprising the steps of:

[0025] (i) determining the in vitro efficacy profile of the drug candidate for a first and a second species according to the method as described herein; and

[0026] (ii) selecting the drug candidate for further development if the efficacy profile of the drug candidate is favourable.

[0027] In one embodiment, the genetic similarity between the first and the second species is high, in particular more than 90%.

[0028] In one embodiment, the first species is cynomolgus monkey (Macaca fascicularis) and the second species is human (Homo sapiens).

[0029] In one embodiment, the drug candidate comprises a nucleic acid molecule or targets a specific nucleic acid sequence.

[0030] In one embodiment, the drug candidate comprises at least one nucleic acid molecule such as a RNAi agent or an antisense oligonucleotide.

[0031] In one embodiment, the further development comprises determining the in vivo efficacy and/or toxicity profile of the drug candidate.

[0032] In one embodiment, provided is a method for determining the potential in vivo efficacy of a drug candidate wherein the in vitro efficacy profile of a drug candidate is determined as described herein and wherein the in vitro efficacy profile is indicative for in vivo efficacy.

[0033] In one embodiment, the in vitro efficacy profile of a drug candidate is indicative for in vivo efficacy in human (Homo sapiens).

[0034] In one embodiment, the in vitro efficacy profile of a drug candidate is indicative for in vivo efficacy in cynomolgus monkey (Macaca fascicularis).

[0035] In one embodiment, the in vivo efficacy profile is determined in at least one species.

[0036] In one embodiment, the in vivo efficacy profile is determined in cynomolgus monkey (Macaca fascicularis).

[0037] In one embodiment, the determined in vitro efficacy profile and/or in vivo efficacy profile of the drug candidate is indicative for in vivo efficacy in human (Homo sapiens).

[0038] In one embodiment, the determined in vitro efficacy profile and the in vivo efficacy profile of the drug candidate as assessed in cynomolgus monkey is indicative for in vivo efficacy in human (Homo sapiens).

[0039] In one embodiment, the differentiation medium is basal medium supplemented with 20 ng/ml BDNF, 10 ng/ml GDNF, 0.5 mM cAMP, and 100 .mu.M ascorbic acid phosphate.

[0040] In one embodiment, the cell cultures are produced sequentially for different species.

[0041] In one embodiment, provided is the methods and uses essentially as described herein.

DESCRIPTION OF THE FIGURES

[0042] FIG. 1: The upper strand illustrates the region of the SNHG14 transcript downstream of SNORD109B (UBE3A-ATS) where the black boxes indicate the location of the tested mouse oligonucleotides. The lower strand illustrates the UBE3A coding region, where the black boxes indicate exons. Exon 1 is located around 160 kb. The oligonucleotides are placed in the antisense region of Exon 9 (positioned at .about.97 kb), Exon 10 (positioned at .about.92 kb), Exon 13 (positioned at .about.77 kb) and the 5' end of Exon 16 (positioned at .about.60 kb).

[0043] FIG. 2: Representation of the ability of the oligonucleotides, tested in Example 2, to induce re-expression of UBE3A in human neuronal cell cultures. Oligonucleotides complementary to the region of human SNHG14 long non-coding RNA between SNORD109B and the region upstream of the UBE3A coding region (position 1 to 55318 of SEQ ID NO: 1) are indicated with .circle-solid. nonoverlap. Oligonucleotides complementary to the region of human SNHG14 long non-coding RNA which is antisense to the UBE3A pre-mRNA (position 55319 to 141053 of SEQ ID NO: 1) are indicated with .tangle-solidup. overlap. Oligonucleotides from Table 3 with conservation to human and rhesus monkey are indicated at the bottom of each plot as . Conservation between human:rhesus:mouse is indicated by . The oligonucleotide concentrations were 0.2, 1 and 5 microM as indicated in the right hand side each plot.

[0044] FIG. 3: Schematic representation of a screening strategy to assess efficacy of a drug candidate on primate in vitro cell culture models followed by in vivo studies on a primate species before assessing drug efficacy on humans. Cynomolgus monkey and human IPSC-derived neurons are used as in vitro model to assess target engagement and efficacy of oligonucleotides targeting UBE3A antisense. Candidates with favorable efficacy profile are prioritized prior to in vivo studies.

[0045] FIG. 4: Overview of the different steps carried out for the derivation of neural progenitor cells from cynomolgus IPSCs. A modified version of the dual SMAD inhibition protocol is used for neuralization of primate IPSC lines. MT refers to MT medium; N2B27+SB+LDN refers to N2B27 medium supplemented with SB-431542 and LDN-193189; N2B27+FEB refers to N2B27 medium supplemented with FGF, EGF and BDNF.

[0046] FIG. 5A and FIG. 5B: Cyno and human neural precursors display a similar staining positive for Sox2 and Nestin and respective differentiated neurons stain positive for Map2.

[0047] FIG. 6A, FIG. 6B, FIG. 6C, FIG. 6D: The applied directed-differentiation method induces a comparable cross-species neuronal differentiation. FIG. 6A) Cyno IPSCs were differentiated in basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid phosphate (BGAA) for 14 days. RNA from cyno IPSCs and day 14 NCs was isolated and the expression of the indicated markers was analyzed by qPCR. Data are normalized to the housekeeping gene GAPDH and are presented relative to expression levels in cyno IPSCs. FIG. 6B) RNA was isolated from human PSCs and human NCs differentiated for 14 days in basal medium supplemented with BGAA and the expression of the indicated markers was analyzed by RNA sequencing. Data are presented as RPKM values. FIG. 6C) RNA was isolated from human PSCs and human NCs differentiated for 14 days in basal medium supplemented with BGAA and the expression of the indicated markers was analyzed by qPCR. Data are normalized to the housekeeping gene GAPDH and are presented relative to expression levels in human PSCs. FIG. 6D) RNA from cyno (left panel) and human (right panel) NCs differentiated in basal medium supplemented with BGAA for 14 days has been isolated and expression of UBE3A and UBE3A-ATS transcripts has been analyzed by qPCR. Left panel: Data are normalized to the housekeeping gene TBP and presented relative to cyno IPSCs. Right panel: Data are normalized to the housekeeping gene GAPDH and presented relative to human PSCs.

[0048] FIG. 7: Schematic depiction of experimental layout for comparison of 4 differentiation methods (A-D) to derive robust neuronal cultures from cyno neural precursors, which is amenable for compound screening. MI refers to Mitotic inhibitor.

[0049] FIG. 8: Image-based comparison of the 4 neuronal differentiation methods as described in FIG. 7. NCs were fixed at day 35 of differentiation and stained for Sox2 (marker for Glia and NSCs) depicted in magenta and Map2 (marker for NCs) depicted in green.

[0050] FIG. 9: To test the feasibility of dissociating and reseeding human iPSC-derived differentiated NCs, two cell lines were differentiated in parallel. For each, cells were differentiated directly (i.e. without replating) for 6 weeks, or dissociated and reseeded at day 21 and then cultured for a further 3 weeks. Immunofluorescent staining for neuronal (MAP2) and glial (GFAP) markers shows that the dissociating and reseeding (replating) does not hinder the ability of the cells to differentiate.

[0051] FIG. 10: Quantification of the extend of differentiation in two different iPSC-derived cell lines with and without dissociation and reseeding (replating). HuC/D is a marker expressed by neurons and was detected by immunofluorescence staining in cultures differentiated for a total of 6 weeks, and quantified by high content imaging. The data show that replating does not significantly change the extent of neuronal differentiation.

[0052] FIG. 11: The expression of the microtubule associated protein Tau and two of its phosphorylated forms was analyzed. The constant expression and extent of phosphorylation in two cell lines with and without replating proves that replating (dissociating and reseeding) does not disrupt the cytoskeletal characteristics of human iPSC-derived NCs, suggesting that physiological features will not be altered by dissociating and reseeding the NCs.

[0053] FIG. 12: Schematic illustration of the workflow enabling a constant supply of robust primate neuronal cultures for screening in a high-throughput format. SFA refers to basal medium supplemented with Shh, FGF8 and ascorbic acid phosphate; BGAA refers to basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid phosphate; arrows signify dissociation and reseeding (replating).

[0054] FIG. 13A, FIG. 13B, FIG. 13C, FIG. 13D: Target engagement of tested compounds on standardized cell cultures of uniformly distributed neural cells (NCs) from human and Cynomolgus monkey. Two concentrations (low: 0.02 .mu.M; high: 2 .mu.M) of antisense oligonucleotides directed against UBE3A-antisense transcript (antisense) with known target engagement in human were tested. The NC cultures derived from Cynomolgus monkey in A and B display a comparable target engagement as compared to the NC cultures derived from human in C and D. Treatment of cyno and human NCs at indicated concentration results in a reduction of the UBE3A-antisense transcript accompanied with an upregulation of the UBE3A transcript (sense). The cell cultures were produced according to the workflow as included in FIG. 12.

DETAILED DESCRIPTION

[0055] The term "antisense oligonucleotide" as used herein is defined as oligonucleotides capable of modulating expression of a target gene by hybridizing to a target nucleic acid, in particular to a contiguous sequence on a target nucleic acid. The antisense oligonucleotides are not essentially double stranded and are therefore not siRNAs. Preferably, the antisense oligonucleotides of the present invention are single stranded.

[0056] As used herein, the term "basal medium" refers to a defined medium composed of equal volumes of DMEM:F12 Glutamax medium and Neurobasal medium (Gibco, Invitrogen), supplemented with 1.times.B27 (Gibco, Invitrogen), 1.times.N2 (Gibco, Invitrogen), 0.1 mM beta-mercaptoethanol (Gibco, Invitrogen). The term "BGAA" refers to basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science), and 100 .mu.M ascorbic acid phosphate (Sigma). The term "SFA" refers to basal medium supplemented with 200 ng/ml Shh, 100 ng/ml FGF8 and 100 .mu.M ascorbic acid phosphate.

[0057] The term "contiguous nucleotide sequence" as used herein refers to the region of the oligonucleotide which is complementary to the target nucleic acid. The term is used interchangeably herein with the term "contiguous nucleobase sequence" and the term "oligonucleotide motif sequence". In some embodiments all the nucleotides of the oligonucleotide are present in the contiguous nucleotide sequence. In some embodiments the oligonucleotide comprise the contiguous nucleotide sequence and may, optionally comprise further nucleotide(s), for example a nucleotide linker region which may be used to attach a functional group to the contiguous nucleotide sequence. The nucleotide linker region may or may not be complementary to the target nucleic acid.

[0058] As used herein, the term "defined medium" or "chemically defined medium" refers to a cell culture medium in which all individual constituents and their respective concentrations are known. Defined media may contain recombinant and chemically defined constituents.

[0059] As used herein the terms "differentiating" and "differentiation" refers to one or more steps to convert a less-differentiated cell into a more-differentiated cell, in particular a postmitotic tissue-specific cell type, e.g., to convert a NPC into a NC. Differentiation of NPCs to NCs can be induced inter alia by adding one or several differentiating agents to the cell culture medium.

[0060] As used herein the term "efficacy profile" or "efficacy" is defined as generally understood by the skilled person to comprise an assessment of the efficacy of a drug candidate based on exposure of a test system, e.g., a cell culture or an organism, with the drug candidate, in particular in different concentrations and/or with different routes of administration, followed by the determination of resulting cellular and/or physiological effects correlating to the desired effect of the drug candidate. Parameters to determine a cellular and/or physiological effect are defined in context with the respective drug candidate, comprising parameters correlating with the desired phenotypic effect of the drug candidate on the test system or an organism. Preferably, more than one parameter including but not limited to survival, cell viability, morphology, expression and/or expression level of specific genes and protein synthesis is recorded to establish an efficacy profile of a drug candidate. In one aspect of the invention, establishing the efficacy profile includes assessing target engagement.

[0061] "Expression markers" or "markers" can be used to determine the identity of a cell type. A certain informative DNA sequence of a cell specific gene is transcribed into mRNA and usually is subsequently translated into a protein (its gene product) which exerts a certain function in a cell. The expression of a marker can be detected and quantified on the RNA level or on the protein level by methods known in the art. IPSC cell markers are known in the art and include but are not limited to TRA-1-60, TRA-1-81, Ecat1, Nanog, Oct4/POU5F1, Sox2, Rex1/Zfp-42 and UTF1, or any combinations thereof. NPC cell markers are known in the art and include but are not limited to Sox2, Nestin, Sox1, Pax6, Dach1. NC cell markers are known in the art and include but are not limited to MAP2, .beta.-III-Tubulin, DCX/Doublecortin, SYN 1/Synapsin 1 and GPHN/Gephyrin.

[0062] As used herein, the term "genetic distance" shall be understood as a measure of the genetic divergence between two species, two genomes or two populations. The genetic distance, e.g., between different species, can be determined by methods known in the art including but not limited to determining the Nei's standard distance, the Goldstein distance or the Rynolds/Weir/Cockerham's genetic distance. Genetic distance can be calculated using software known to the art including but not limited to POPTREE2 or DISPAN. The "genetic similarity" is high when the genetic distance is low.

[0063] As used herein, the following abbreviations are used; fibroblast growth factor (FGF), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), sonic hedgehog (shh), fibroblast growth factor 8 (FGF8), brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), cyclic adenosine monophosphate (cAMP), Rho-associated coiled-coil forming protein serine/threonine kinase (ROCK).

[0064] As used herein, the term "growth factor" means a biologically active polypeptide or a small molecule compound which causes cell proliferation, and includes both growth factors and their analogs.

[0065] "High-throughput screening" as used herein shall be understood to signify that a relatively large number of different disease model conditions and/or chemical compounds can be analyzed and compared with the novel assay described herein. Typical, such high-throughput screening is performed in multi-well microtiter plates, e.g., in a 96 well plate or a 384 well plate or plates with 1536 or 3456 wells.

[0066] "LNA nucleosides" are modified nucleosides which comprise a linker group (referred to as a biradicle or a bridge) between C2' and C4' of the ribose sugar ring of a nucleotide. These nucleosides are also termed bridged nucleic acid or bicyclic nucleic acid (BNA) in the literature.

[0067] As used herein, the terms "uniformly distributed", "uniform distribution" or "homogenous distribution" refers as generally understood by the skilled person to the distribution of an entity in a 1-, 2- or multidimensional space, in particular to the distribution of cells on the 2-dimensional surface of a cell culture support. A uniform distribution is established on a 2-dimensional cell culture surface if the mean cell count per area is essentially constant over the whole cell culture surface. The distribution of cells can be assessed, e.g., by nuclear staining and determining the number of cell nuclei per area using fluorescence microscopy. Indicators for non-homogenous distribution of cells include, e.g., clumps of cells, a significant number of overlapping cell nuclei, or a significant portion of the cell culture area devoid of cells. A cell culture with uniformly distributed cells, of one or more cell types, is referred to as "standardized" as used herein. A "standardized cell culture" or "standardized NC culture" refers to a cell culture produced according to the present invention wherein the distribution of the cells is essentially uniform, i.e., the cells are uniformly distributed and the cell cultures are characterized by uniform distribution of the cells, wherein the cell culture may include one or more cell types. Accordingly, a cell culture is considered to be standardized if the cells display a homogenous distribution as assessed, e.g., by nuclear staining and determining the number of cell nuclei per area.

[0068] The term "modified internucleoside linkage" as used herein is defined as generally understood by the skilled person as linkages other than phosphodiester (PO) linkages that covalently couple two nucleosides together. Nucleotides with modified internucleoside linkage are also termed "modified nucleotides". The modified internucleoside linkage increases the nuclease resistance of the oligonucleotide compared to a phosphodiester linkage. For naturally occurring oligonucleotides, the internucleoside linkage includes phosphate groups creating a phosphodiester bond between adjacent nucleosides. Modified internucleoside linkages are particularly useful in stabilizing oligonucleotides for in vivo use, and may serve to protect against nuclease cleavage at regions of DNA or RNA nucleosides in the oligonucleotide of the invention, for example within the gap region of a gapmer oligonucleotide, as well as in regions of modified nucleosides.

[0069] As used herein, the term "MT medium" refers to a defined medium that contains Dulbecco's Modified Eagle Medium with Ham's F12 Nutrient Mixture (DMEM/F12) with 2.5 mM GlutaMAX.TM., 7 .mu.g/ml insulin, 450 .mu.M monothioglycerole, 1.times. Lipid concentrate, 5 mg/ml BSA, 14 ng/ml sodium selenite, 1.times. non-essential amino acids, 2 mg/ml heparin, 15 .mu.g/ml transferrin, and 220 .mu.M ascorbic acid-2-phosphate.

[0070] As used herein, "neuronal precursor cells" or "NPCs" refers to a subset of multipotent cells, which were derived from IPSCs and express some neural progenitor cell markers including, for example, Nestin. NPCs can be produced, inter alia, according to the methods as described in Costa et al. Cell Rep 2016; 15:86-95 and Dunkley et al. Proteomics Clin Appl 2015; 7-8:684-94 which are incorporated herein by reference in their entirety or according to the methods as described herein. NPCs can be expanded indefinitely and may differentiate into neurons or glial cells (e.g., astrocytes and oligodendrocytes). The term "patient specific NPCs" refers to NPCs obtained from patient IPSCs that have been reprogrammed from somatic cells of a patient. "NPCs obtained from a healthy individual" as used herein refers to NPCs differentiated from IPSCs obtained from somatic cells of an individual that is apparently healthy and not suspected to suffer from any disorder or disease.

[0071] As used herein, "neuronal cells" or "NCs" refer to tissue-specific cells from the neuronal lineage. NCs can be differentiated in vitro from NPCs using specific cell culture conditions, e.g., by withdrawal of growth factors or by addition of one or more differentiating agents, as described herein.

[0072] The term "non-human primate" or "NHP" as used herein refers to species belonging to the order of primates with the exception of Homo sapiens. In particular, NHP species according to the methods disclosed in the present invention include but are not limited to Pan troglodytes, Pan paniscus, Hylobates lar, Gorilla gorilla, Pongo abelii, Pongo pygmaeus, Cercopithecus mitis, Cercopithicus neglectus, Chlorocebus aethiops, Chlorocebus sabaeus, Colobus guereza, Lophocebus aterrimus, Macaca arctoides, Macaca assamensis, Macaca fascicularis (Cynomolgus monkey), Macaca fuscata, Macaca mulatta (Rhesus monkey), Macaca nemestrina, Macaca silenus, Mandrillus leucophaeus, Mandrillus sphinx, Macaca thibetana, Papio anubis, Papio cynocephalus, Papio hamadryas, Papio papio, Papio ursinus, Presbytis entellus, Theropithecus gelada, Aotus azarae, Aotus nancymaae, Aotus nigriceps, Aotus trivirgatus, Aotus vociferans, Ateles belzebuth, Ateles fusciceps, Callithrix jacchus, Callicebus moloch, Cebuella pygmaea, Cebus apella, Leontopithecus rosalia, Pithecia pithecia, Saguinus fuscicollis, Saguinus geoffroyi, Saguinus labiatus, Saguinus mystax, Saguinus Oedipus and Saimiri sciureus.

[0073] The term "cyno" as used herein is an abbreviation for Cynomolgus monkey and/or refers to material derived from Cynomolgus monkeys including but not limited to cells, tissues, organs, blood or cells derived therefrom.

[0074] "Nucleotides" are the building blocks of oligonucleotides and polynucleotides, and for the purposes of the present invention include both naturally occurring and non-naturally occurring nucleotides. In nature, nucleotides, such as DNA and RNA nucleotides comprise a ribose sugar moiety, a nucleobase moiety and one or more phosphate groups (which is absent in nucleosides). Nucleosides and nucleotides may also interchangeably be referred to as "units" or "monomers".

[0075] As used herein, the term "nucleotide sequence derived from a human genome" means that the respective nucleotide sequence is derived from a human genome reference, i.e. at least a subpopulation of the global human population comprises the respective nucleotide sequence in the genome. Furthermore, as used herein the term "nucleotide sequence derived from a human genome" is used for sequences assigned to a human genome with highest maximal score using the NCBI/Blast database and algorithm (Zheng Zhang, Scott Schwartz, Lukas Wagner, and Webb Miller (2000), "A greedy algorithm for aligning DNA sequences", J Comput Biol 2000; 7(1-2):203-14.). Without being bound to theory it is assumed that the alignment Score of a query sequence is higher for a human reference sequence compared to a non-human reference sequence if the query sequence is derived from a human genome.

[0076] The term "modified nucleoside" or "nucleoside modification" as used herein refers to nucleosides modified as compared to the equivalent DNA or RNA nucleoside by the introduction of one or more modifications of the sugar moiety or the (nucleo)base moiety. In a preferred embodiment the modified nucleoside comprises a modified sugar moiety. The term modified nucleoside may also be used herein interchangeably with the term "nucleoside analogue" or modified "units" or modified "monomers".

[0077] The term "modified internucleoside linkage" is defined as generally understood by the skilled person as linkages other than phosphodiester (PO) linkages, that covalently couples two nucleosides together. Nucleotides with modified internucleoside linkage are also termed "modified nucleotides". In some embodiments, the modified internucleoside linkage increases the nuclease resistance of the oligonucleotide compared to a phosphodiester linkage. For naturally occurring oligonucleotides, the internucleoside linkage includes phosphate groups creating a phosphodiester bond between adjacent nucleosides. Modified internucleoside linkages are particularly useful in stabilizing oligonucleotides for in vivo use, and may serve to protect against nuclease cleavage at regions of DNA or RNA nucleosides in the oligonucleotide of the invention, for example within the gap region of a gapmer oligonucleotide, as well as in regions of modified nucleosides.

[0078] As used herein, the term "N2B27" refers to a defined medium composed of equal volumes of DMEM:F12 (Gibco, Invitrogen) supplemented with N2 and B27 (both from Gibco, Invitrogen).

[0079] The term "oligonucleotide" as used herein is defined as it is generally understood by the skilled person as a molecule comprising two or more covalently linked nucleosides. Such covalently bound nucleosides may also be referred to as nucleic acid molecules or oligomers. Oligonucleotides are commonly made in the laboratory by solid-phase chemical synthesis followed by purification. When referring to a sequence of the oligonucleotide, reference is made to the sequence or order of nucleobase moieties, or modifications thereof, of the covalently linked nucleotides or nucleosides. The oligonucleotide may comprise one or more modified nucleosides or nucleotides. The term "antisense oligonucleotide" as used herein is defined as oligonucleotides capable of modulating expression of a target gene by hybridizing to a target nucleic acid, in particular to a contiguous sequence on a target nucleic acid. The antisense oligonucleotides are not essentially double stranded and are therefore not siRNAs. Preferably, the antisense oligonucleotides are single stranded.

[0080] As used herein, the term "reprogramming" refers to one or more steps needed to convert a somatic cell to a less-differentiated cell, for example for converting a fibroblast cell, adipocytes, keratinocytes or leucocyte into a NPC. "Reprogrammed" cells refer to cells derived by reprogramming somatic cells as described herein.

[0081] The term "small molecule", or "small compound", or "small molecule compound" as used herein, refers to organic or inorganic molecules either synthesized or found in nature, generally having a molecular weight less than 10,000 grams per mole, optionally less than 5,000 grams per mole, and optionally less than 2,000 grams per mole.

[0082] The term "somatic cell" as used herein refers to any cell forming the body of an organism that are not germline cells (e.g., sperm and ova, the cells from which they are made (gametocytes)) and undifferentiated stem cells.

[0083] The term "stem cell" as used herein refers to a cell that has the ability for self-renewal and differentiation. An "undifferentiated stem cell" as used herein refers to a stem cell that has not undergone differentiation. As used herein, "pluripotent stem cells" or "PSCs" refers to stem cells that can give rise to cell types of the three germlayers (endoderm, ectoderm, mesoderm) as well as the germline. Pluripotent stem cells (PSCs) include but are not limited to "embryonic stem cells" ("ESCs") and "induced pluripotent stem cells" ("IPSCs"). The terms "hIPSCs" and "cIPSCs" refer to IPSCs derived from human cells and to IPSCS derived from Cynomolgus monkey cells, respectively.

[0084] As used herein the term "substantial loss of cell viability" refers to a reduction of cell viability upon application of distinct cell culture conditions or manipulating the cells in a defined process, in particular in connection to dissociating cells from the cell culture support. In one embodiment, substantial loss of cell viability means that more than 5% of the cells become non-viable and/or undergo apoptosis. In further embodiments, substantial loss of cell viability means that more than 10%, more than 15%, more than 20% or more than 25% of the cells become non-viable and/or undergo apoptosis. Accordingly, in one embodiment, the term "essentially remain viable" means that more of 95% of the cells remain viable. In further embodiments, essentially remain viable means that more than 90%, more than 85%, more than 80% or more than 75% of the cells remain viable.

[0085] A "suitable medium for differentiation", also depicted as "differentiation medium", as used herein refers to any chemically defined medium useful for differentiation of NPCs to NCs. A differentiation medium as described herein contains at least one "differentiating agent". Differentiating agents include but are not limited to biologically active polypeptides or a small molecule compounds which cause cell differentiation.

[0086] The "target" refers to the protein which it is desired to modulate. A "target nucleic acid" is the intended target which the oligonucleotide of the invention hybridizes to, and may for example be a gene, a RNA, a non-coding RNA, a long non-coding RNA, a mRNA, and pre-mRNA, a mature mRNA or a cDNA sequence. In some embodiments the target nucleic acid is a non-coding RNA or a long non-coding RNA, or a subsequence thereof. For particular in vivo or in vitro application, the oligonucleotide of the invention is capable of decreasing the level of the SNHG14 transcript downstream of SNORD109B of and thereby relieving the suppression of the paternal UBE3A transcript in the intended target cell. The contiguous sequence of nucleobases of the oligonucleotide of the invention is complementary to the target nucleic acid, as measured across the length of the oligonucleotide, optionally, with the exception of one or two mismatches, and, optionally, excluding nucleotide based linker regions which may link the oligonucleotide to an optional functional group such as a conjugate. The oligonucleotide comprises a contiguous nucleotide sequence which is complementary to or hybridizes to a sub-sequence of the target nucleic acid molecule.

[0087] The term "target sequence" as used herein refers to a sequence of nucleotides present in the target nucleic acid which comprises the nucleobase sequence which is complementary to the oligonucleotide of the invention. In some embodiments, the target sequence consists of a region on the target nucleic acid which is complementary to the contiguous nucleotide sequence of the oligonucleotide of the invention. In some embodiments the target sequence is longer than the complementary sequence of a single oligonucleotide, and may, for example represent a preferred region of the target nucleic acid which may be targeted by several oligonucleotides of the invention. The oligonucleotide of the invention comprises a contiguous nucleotide sequence which is complementary to the target nucleic acid, such as a target sequence. The oligonucleotide comprises a contiguous nucleotide sequence of at least 8 nucleotides which is complementary to or hybridizes to a target sequence present in the target nucleic acid molecule. The contiguous nucleotide sequence (and therefore the target sequence) comprises of at least 8 contiguous nucleotides, such as 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 contiguous nucleotides, such as from 12-25, such as from 14-18 contiguous nucleotides.

[0088] The term a "target cell" as used herein refers to a cell which is expressing the target nucleic acid. In some embodiments the target cell may be in vivo or in vitro. In some embodiments the target cell is a mammalian cell such as a rodent cell, such as a mouse cell or a rat cell, or a primate cell such as a monkey cell or a human cell. In one embodiment the target cell is a neuronal cell (NC).

[0089] As used herein the term "toxicity profile" or "toxicity" is defined as generally understood in the art to comprise a toxicological assessment of a potential harmful or non-harmful substance based on exposure of a test system, e.g., a cell culture or an organisms, with the substance, in particular in different concentrations and/or with different routes of administration, followed by the determination of resulting cellular and/or physiological effects, e.g., cell survival or health. Parameters to determine cellular and/or physiological effects are well known in the art including but not limited to survival, cell viability, morphology, expression and/or expression level of certain genes and protein synthesis. Preferably, more than one parameter is recorded to establish a toxicity profile of a substance with unknown toxicity. The toxicity profile of a drug candidate is used in the art to select drug candidates for further development, e.g., in vivo testing on primates or humans.

[0090] The present invention provides a novel method for producing reproducible and standardised differentiated NC cultures from different primate species, including human, which can be used for in vitro high-throughput testing of drug candidates. The method comprises providing primate NPCs, differentiating the NPCs to NCs, and capacitating the cell cultures for high-throughput screening of drug candidates by dissociating the differentiated primate NCs and reseeding the cells in a suitable cell culture format without significant loss of cell viability, and pursuing differentiation of the NCs. The primate NPCs can be derived from IPSCs or transdifferentiated cells, which both can be generated from somatic cells. Preferably said somatic cells are primate cells including human somatic cells.

[0091] In one embodiment provided is an in vitro method for producing cell cultures of uniformly distributed differentiated neuronal cells (NCs) from different primate species, the method comprising the steps of

[0092] a) providing neuronal precursor cells (NPCs); and

[0093] b) differentiating the NPCs to NCs comprising the steps of

[0094] (i) dissociating the differentiated NCs from its support between day 20 and 45 of differentiation; and

[0095] (ii) reseeding the cells in a suitable cell culture format and continuing differentiation of the NCs for 4 to 15 days.

[0096] To achieve the inventive method described here, it was necessary to bypass some of the existing limitations of NC cultures. It is widely accepted that in contrast to NPCs, which can be expanded indefinitely either as adherent or floating cultures, differentiating NCs are critically dependent on interaction with a matrix usually consisting of a biopolymer coating of a cell culture plate. Furthermore, differentiated NCs become sensitive to cellular stress and, therefore, passaging of differentiated cells is considered to be detrimental to the cells. The innovative method according to the present invention discloses cell culture conditions whereby uniform NC cultures can be produced from different primate species wherein the NCs are dissociated from its support between day 10 and 40 of differentiation without substantial loss of viability. The NCs can be reseeded in a suitable cell culture format and the differentiation continued. The dissociation and reseeding results in a homogenous distribution of the differentiated NCs over the cell culture area. Furthermore, the method of the present invention allows changing the cell culture format at a late stage of NC differentiation. In further embodiments, step b)(i) comprises dissociating the differentiated NCs from its support between about day 25 and about day 40, between about day 28 and about day 30, at about day 28 or at about day 30 of differentiation.

[0097] Accordingly, one aspect of the present invention is a method as described herein to produce uniform differentiated NC cultures. In one embodiment, step b) comprises differentiating the NPCs to neural cells (NCs) comprising the steps of (i) dissociating the differentiated NCs from its support between day 20 and 45 of differentiation; and (ii) reseeding the cells in a suitable cell culture format and continuing differentiation of the NCs for 4 to 10 days, wherein the NCs essentially remain viable. Said dissociation and reseeding step is critical to obtain a uniform distribution of differentiated primate NCs across the area of a cell culture surface, e.g., a cell culture well and/or for harvesting differentiated primate NCs without substantial loss of viability. Consequently, differentiated primate NC culture assays produced according to the present invention display more evenly distributed cells and are better suited for high-throughput assays compared to cell cultures produced without dissociation and reseeding. Importantly, the cell culture format can be changed at a late stage of NC differentiation. This is in contrast to methods of the art, wherein the final cell culture format has to be employed at an early time point. Indeed, being able to change the cell culture format allows for considerable logistic flexibility.

[0098] The highly reproducible cell cultures of primate NCs are well standardised and can be used in compound screening assays including but not limited to in vitro efficacy assessment of drug candidates. Furthermore, the cell cultures as described herein can be used for selecting drug candidates, in particular for selecting a drug candidate for further development as described herein. In one embodiment, the cell cultures according to the invention are used to determine the in vitro efficacy profile of a drug candidate as described herein. In a further embodiment the in vitro efficacy profile of a drug candidate is determined prior to the determination of an in vivo efficacy profile as described herein. In a further embodiment, the cell cultures according to the invention are used to determine the in vitro toxicity profile of a drug candidate as described herein.

[0099] Accordingly, in one embodiment, differentiated primate NCs are dissociated from the cell culture vessel, wherein the NCs essentially remain viable. The dissociated NCs can be reseeded in a desired cell culture format at a cell density optimized to the needs of a given cell culture assay. In one embodiment, the dissociation and reseeding conditions as described in the present invention can be applied to different primate species. In one embodiment, the method according to the present invention can be used for inter-species comparison of the efficacy profile of a drug candidate, wherein the cell cultures are produced individually from cells of at least two species, wherein essentially the same conditions are applied to the cultures for all species and wherein the efficacy profile is determined and compared for all species. It is within the scope of the present invention to produce standardised cell culture assays deriving from at least one primate species, at least two primate species or at least three primate species using essentially the same cell culturing conditions and to compare and integrate the results as determined by the assay readout to determine a comprehensive efficacy profile of at least one drug candidate.

[0100] In one embodiment the NPCs are generated from IPSCs derived from reprogrammed somatic cells. Reprogramming of somatic cells to IPSCs can be achieved by introducing specific genes involved in the maintenance of IPSC properties. Genes suitable for reprogramming of somatic cells to IPSCs include, but are not limited to Oct4, Sox2, Klf4 and C-Myc and combinations thereof. In one embodiment the genes for reprogramming are Oct4, Sox2, Klf4 and C-Myc. Combinations of genes for transdifferentiating somatic cells to NPCs are described in WO2012/022725 which is herein included by reference.

[0101] Internal organs, skin, bones, blood and connective tissue are all made up of somatic cells. Somatic cells used to generate IPSCs include but are not limited to fibroblast cells, adipocytes and keratinocytes and can be obtained from skin biopsy. Other suitable somatic cells are leucocytes, erythroblasts cells obtained from blood samples or epithelial cells or other cells obtained from blood or urine samples and reprogrammed to IPSCs by the methods known in the art and as described herein. The somatic cells can be obtained from a healthy individual or from a diseased individual. The genes for reprogramming as described herein are introduced into somatic cells by methods known in the art, either by delivery into the cell via reprogramming vectors or by activation of said genes via small molecules. Methods for reprogramming comprise, inter alia, retroviruses, lentiviruses, adenoviruses, plasmids and transposons, microRNAs, small molecules, modified RNAs and recombinant proteins. In one embodiment, a lentivirus is used for the delivery of genes as described herein. In another embodiment, Oct4, Sox2, Klf4 and C-Myc are delivered to the somatic cells using Sendai virus particles. In addition the somatic cells can be cultured in the presence of at least one small molecule. In one embodiment, said small molecule comprises an inhibitor of the Rho-associated coiled-coil forming protein serine/threonine kinase (ROCK) family of protein kinases. Non-limiting examples of ROCK inhibitors comprise fasudil (1-(5-lsoquinolinesulfonyl) homopiperazine), Thiazovivin (N-Benzyl-2-(pyrimidin-4-ylamino) thiazole-4-carboxamide) and Y-27632 ((+)-(R)-trans-4-(1-aminoethyl)-N-(4-pyridyl) cyclo-hexanecarboxamide dihydrochloride). Resulting IPSCs can be induced to differentiate into NPCs. In one embodiment, the IPSCs are induced to differentiate into NPCs.

[0102] In one embodiment, primate NPCs are generated from IPSCs by dual SMAD inhibition. In one embodiment NPCs are generated from IPSCs by contacting the cells with SB-431542 (Calbiochem) and LDN-193189 (Calbiochem). In a particular embodiment NPCs are generated from IPSCs by contacting the cells with 5 ng/ml FGF (Peprotech), 10 .mu.M SB-431542 (Calbiochem) and 100 nM LDN-193189 (Calbiochem). Resulting primate NPCs can be expanded in basal medium supplemented with FGF, EGF and BDNF. In one embodiment, NPCs are expanded in basal medium supplemented with 10 ng/ml FGF (Peprotech), 10 ng/ml EGF (RnD), and 20 ng/ml BDNF (Peprotech). Continued passaging in basal medium supplemented with FGF, EGF and BDNF leads to a stable neural progenitor cell line (NPC line). A stable NPC line is defined by its capacity to self-renew and by the expression of the developmental stage-specific markers Sox2 and Nestin. Accordingly, in one embodiment, the primate NPCs express Sox2 and Nestin.

[0103] For propagating proliferation of NPCs the cells are grown in an expansion medium comprising a serum free medium supplemented with growth factors. In one embodiment, said growth factors comprise FGF, BDNF and EGF. Accordingly, in one embodiment, the method additionally comprises incubating the cells of step a) under conditions suitable for proliferation of NPCs, e.g., until a defined number of cells per area is reached. Non-limiting examples of expansion media are described herein. In one embodiment, the expansion medium is supplemented with 10-50 ng/ml FGF, 10-50 ng/ml EGF and 1-20 ng/ml BDNF. In a particular embodiment, the NPC expansion medium is basal medium supplemented with 10 ng/ml FGF2, 10 ng/ml EGF and 20 ng/ml BDNF. NPCs can be produced in unrestricted quantities and are therefore most suitable for high-throughput cell culture assays requiring large numbers of assay plates. Culturing is within the capabilities of the person skilled in the art.

[0104] In one embodiment the primate NPCs are washed with a suitable buffer or medium prior to initializing differentiation to remove any dead cells. Preferably the media are changed in between each step of the cell culture protocol, e.g., the medium is removed, by aspiration or centrifuging the cells and discarding the supernatant and then the medium used in the subsequent step is added to the cells. In one embodiment the cells are washed with a suitable buffer or medium prior to adding the medium of the subsequent step to remove any dead cells and any residual medium or growth factors or cytokines applied in the previous step. Buffers or media useful for washing the cells are known in the art. One example of a suitable buffer for washing the cells is phosphate buffered saline (PBS).

[0105] In one embodiment, the primate NPC cultures are provided at a density of about 5000 cells/cm.sup.2 to about 100000 cells/cm.sup.2. In further embodiments, the primate NPC cultures are provided at a density of about 10000 cells/cm.sup.2 to about 50000 cells/cm.sup.2. In one embodiment, the adherent primate NPC cultures are provided at a density of about 20000 cells/cm.sup.2 to about 40000 cells/cm.sup.2. In one embodiment the adherent primate NPC cultures are provided at a density of about 30000 cells/cm.sup.2. In one embodiment the adherent primate NPC cultures are provided on a Laminin521 support.

[0106] In one embodiment, the primate NPCs obtained by methods known in the art and as described herein are in a next step induced to differentiate to NCs by contacting the cells with Shh (sonic hedgehog), FGF8 (fibroblast growth factor 8) and ascorbic acid phosphate. In one embodiment the NPCs are incubated with a chemically defined medium as described herein comprising Shh, FGF8 and ascorbic acid phosphate. In one embodiment, the medium is supplemented with 50-1000 ng/ml Shh, 25-500 ng/ml FGF8 and 20-200 .mu.M ascorbic acid phosphate. In further embodiments, the cells are contacted with Shh, FGF8 and ascorbic acid phosphate for about 1 day, for about 2 days, for about 3 days, for about 4 days, for about 5 days, for about 6 days, for about 7 days, for about 8 days, for about 9 days or for about 10 days. In a further embodiment, the cells are contacted with Shh, FGF8 and ascorbic acid phosphate for about 5 days to about 10 days. In a particular embodiment, the primate NPCs are cultivated in basal medium supplemented with 200 ng/ml Shh, 100 ng/ml FGF8 and 100 .mu.M ascorbic acid phosphate for about 7 days.

[0107] In one embodiment the cells are replated after induction of neuronal differentiation at a density of about 10000 cells/cm.sup.2 to about 80000 cells/cm.sup.2, about 20000 cells/cm.sup.2 to about 70000 cells/cm.sup.2, about 30000 cells/cm.sup.2 to about 60000 cells/cm.sup.2 or about 40000 cells/cm.sup.2 to about 50000 cells/cm.sup.2. In a particular embodiment the cells are replated after induction of neuronal differentiation at a density of about 45000 cells/cm.sup.2.

[0108] In one embodiment, the cells induced to neuronal differentiation are in a next step differentiated in basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid phosphate for about further 15 days, for about further 16 days, for about further 17 days, for about further 18 days, for about further 19 days, for about further 20 days, for about further 21 days, for about further 22 days, for about further 23 days, for about further 24 days, for about further 25 days, for about further 26 days, for about further 27 days, for about further 28 days, for about further 29 days, for about further 30 days, for about further 31 days, for about further 32 days, for about further 33 days, for about further 34 days or for about further 35 days. In a particular embodiment, the cells are cultivated in basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science), and 100 .mu.M ascorbic acid phosphate (Sigma) for further about 19 days to about 35 days.

[0109] In one embodiment, according to the invention, the differentiated NCs are dissociated from its support and reseeded on a suitable cell culture format as described herein. In further embodiments the NCs, are differentiated in basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid for at least about 10 days, for at least about 11 days, for at least about 12 days, for at least about 13 days, for at least about 14 days, for at least about 15 days, for at least about 16 days, for at least about 17 days, for at least about 18 days, for at least about 19 days, for at least about 20 days, for at least about 21 days, for at least about 22 days, for at least about 23 days, for at least about 24 days, for at least about 25 days, for at least about 26 days, for at least about 27 days or for at least about 28 days before being dissociated and reseeded on a suitable cell culture assay format. In further embodiments, the primate NCs are differentiated in basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid for about 15 to about 30 days, for about 20 to about 25 days, for about 21 to about 23 days before being dissociated and reseeded on a suitable cell culture assay format.

[0110] In one aspect of the invention, the differentiated NCs obtained by the method as described herein are dissociated from the cell culture substrate without substantial loss of cell viability. Accordingly, in one embodiment the differentiated NCs are harvested without substantial loss of cell viability. Cell number can be determined according to conventional methods used in the art including but not limited to counting cell numbers in a haemocytometer or using flow cytometry. Cell viability can be determined according to conventional methods including but not limited to trypan blue and Erythrosin B staining. After dissociation the cells can be reseeded in suitable cell culture wells at a suitable cell density according to the specific need or experimental parameters of the desired assay based on NCs.

[0111] Accordingly, in one embodiment, the differentiated NCs are separated from the cell culture surface by use of a cell detachment solution. In one embodiment, the differentiated NCs are dissociated from its support and reseeded in a suitable cell culture format. In a further embodiment the differentiated NCs are dissociated from its support between about day 20 and about day 45 of differentiation. The number of days of differentiation is counted from the day of initiation of differentiation, e.g., incubating with Shh, FGF8 and ascorbic acid phosphate, wherein the day of addition of Shh, FGF8 and ascorbic acid is counted as day 0. In further embodiments the differentiated NCs are dissociated from its support between about day 15 and about day 50, between about day 28 and about day 30, at about day 25 of differentiation, at about day 26 of differentiation, at about day 27 of differentiation, at about day 28 of differentiation, at about day 29 of differentiation, at about day 30 of differentiation, at about day 31 of differentiation, at about day 32 of differentiation, at about day 33 of differentiation or at about day 34 of differentiation. In one embodiment, the differentiated NCs are dissociated from its support by incubating the cells with a cell detachment solution. In one embodiment the cell detachment solution is Accutase. Accutase is a marine-origin enzyme with proteolytic and collagenolytic activity. In one embodiment the cell detachment solution is added to the differentiated NC cultures and incubated for 1 to 5 minutes, for 2 to 4 minutes, preferentially for 3 minutes. After completion of the incubation time, the Accutase solution is diluted with medium, in particular basal medium. Before reseeding, Accutase containing medium is removed and replenished with fresh basal medium supplemented with growth factors as described herein.

[0112] After detachment the primate NCs can be reseeded in new cell culture containments as, e.g., cell culture wells in a suitable plate format. NCs can be reseeded at any desired density, including low density, medium density and high density. Accordingly, cultures of uniformly distributed differentiated NCs can be generated at a defined and desired density, including low density, medium density and high density. This is in contrast to conventional cell culture methods wherein the cell density is fixed at the beginning of culture and wherein the cell density may change considerably in time due to proliferation of cells or cell death. In one embodiment, the NCs are reseeded at a high density. In further embodiments, NCs are reseeded at a density of about 50000 cells/cm.sup.2 to about 500000 cells/cm.sup.2, at a density of about 75000 cells/cm.sup.2 to about 400000 cells/cm.sup.2, or at a density of about 100000 cells/cm.sup.2 to about 300000 cells/cm.sup.2. In a particular embodiment, the differentiated NCs are reseeded in step b)(ii) at a density of about 200000 cells/cm.sup.2. In one embodiment the cells are dissociated and reseeded after differentiation with BDNF, GDNF, cAMP and ascorbic acid phosphate at a density of about 50000 cells/cm.sup.2 to about 500000 cells/cm.sup.2, about 75000 cells/cm.sup.2 to about 400000 cells/cm.sup.2 or about 100000 cells/cm.sup.2 to about 300000 cells/cm.sup.2. In a particular embodiment the cells are dissociated and reseeded after differentiation with BDNF, GDNF, cAMP and ascorbic acid phosphate at a density of about 200000 cells/cm.sup.2. After dissociation and reseeding (replating), the cells are further differentiated in basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid for about further 1 day, for about further 2 days, for about further 3 days, for about further 4 days, for about further 5 days, for about further 6 days, for about further 7 days, for about further 8 days, for about further 9 days, for about further 10 days, for about further 11 days, for about further 12 days, for about further 13 days, for about further 14 days or for about further 15 days.

[0113] The dissociated and reseeded differentiated NC cultures can be used according to the invention for testing the efficacy of drug candidates. In a particular embodiment the cells are further differentiated in basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science), and 100 .mu.M ascorbic acid phosphate (Sigma) for about 7 days before applying a drug candidate to the cells. Accordingly, the NC cells are ready for screening drug candidates after a total differentiation period of about 10 days to about 50 days, of about 15 days to about 45 days, of about 30 to about 40 days, of about 35 days or about 37 days. In a particular embodiment, the NCs are ready for screening oligonucleotide candidates after a total differentiation period of about 30 to about 40 days.

[0114] In further embodiments, the differentiated NC cultures are ready for treatment with a drug candidate after a total differentiation period of about 28 days, of about 29 days, of about 30 days, of about 31 days, of about 32 days, of about 33 days, of about 34 days, of about 35 days, of about 36 days, of about 37 days, of about 38 days, of about 39 days, of about 40 days, of about 41 days, of about 42 days, of about 43 days, of about 44 days, of about 45 days, of about 46 days, of about 47 days, of about 48 days, of about 49 days, of about 50 days, of about 51 days, of about 52 days, of about 53 days, of about 54 days or of about 55 days. In a further embodiment, treatment with a drug candidate is performed at any step of the cell culture protocols as described herein.

[0115] In a particular embodiment, the primate NPCs are differentiated to NCs in a serum free differentiation medium comprising 20 ng/ml BDNF, 10 ng/ml GDNF, 0.5 mM cAMP and 100 .mu.M ascorbic acid phosphate for about 21 to about 23 days prior to dissociating and reseeding. In a further embodiment, the differentiated NCs are incubated with differentiation medium as described herein for further about 1 day, for further about 2 days, for further about 3 days, for further about 4 days, for further about 5 days, for further about 6 days, for further about 7 days, for further about 8 days, for further about 9 days, for further about 10 days, for further about 11 days, for further about 12 days, for further about 13 days, for further about 14 days, or for further about 15 days after reseeding as described herein. In a particular embodiment, the differentiated NCs are incubated in a serum free differentiation medium comprising 20 ng/ml BDNF, 10 ng/ml GDNF, 0.5 mM cAMP and 100 .mu.M ascorbic acid phosphate for about 7 days after reseeding. Thereafter, the differentiated NCs are ready for treatment with a drug candidate.

[0116] In one aspect of the invention differentiation of the dissociated and reseeded NCs is continued prior to adding a drug candidate to the cell culture medium. Accordingly, in further embodiments, the reseeded NCs are incubated in a differentiation medium as described herein for about 1 day, for about 2 days, for about 3 days, for about 4 days, for about 5 days, for about 6 days, for about 7 days, for about 8 days, for about 9 days, for about 10 days, for about 11 days, for about 12 days, for about 13 days, for about 14 days or for about 15 days prior to adding a drug candidate. In further embodiments step b)(ii) comprises differentiating the NCs after reseeding for further about 1 to about 20 days, for further about 5 to about 10 days, for about 7 days with basal medium comprising 1 to 50 ng/ml BDNF, 1 to 50 ng/ml GDNF and 0.1-10 mM cAMP and 20 to 200 .mu.M ascorbic acid phosphate prior to adding a drug candidate. In a particular embodiment step b)(ii) comprises differentiating the NCs after reseeding for further about 7 days with basal medium comprising 20 ng/ml BDNF, 10 ng/ml GDNF and 0.5 mM cAMP and 100 .mu.M ascorbic acid phosphate prior to adding a drug candidate.

[0117] In one embodiment, the drug candidate is added to the cell culture medium after dissociating and reseeding the differentiated NCs. In further embodiments, the drug candidate is added to the cell culture medium about 1 day to about 15 days, about 5 days to about 10 days or about 7 days after reseeding the differentiated NCs. In a particular embodiment, the drug candidate is added to the cell culture medium about 7 days after reseeding the differentiated NCs.

[0118] In one embodiment the primate NPCs are differentiated to NCs according to the methods as described herein. In one embodiment, the neuronal identity is assessed with expression markers associated with cellular and/or metabolic functions of neurons. Typical neuronal markers include but are not limited to MAP2, HuC/D, Nestin, .beta.-111-Tubulin, DCX/Doublecortin, SYN 1/Synapsin 1 and GPHN/Gephyrin. The expression markers associated with neuronal identity can be expressed at a lower level in NCs derived from NPCs compared to the expression level in primary neurons or neural tissue. The normalized expression level of neuronal expression markers in NSC-derived NCs can be 10000.times. lower, or 1000.times. lower, or 100.times. lower, or 10.times. lower, or 2.times. lower compared to the expression level of the respective markers in primary neurons or neural tissue. The fold change of expression level of neuronal expression markers between NPC-derived NCs and primary neurons can be different for different expression markers. Normalization can be achieved by relating the absolute expression level of a given marker to a suitable house-keeping gene, e.g., GAPDH or TBP.

[0119] In one embodiment, the innovative method of the present invention is used to generate robust differentiated NC cultures with homogenous NC distribution for different primate species including but not limited to human (Homo sapiens), Cynomolgus monkey (Macaca fascicularis) and Rhesus monkey (Macaca mulatta). Essentially the same cell culture conditions can be applied to all primate species.

[0120] Without being bound theory, the present invention distinguishes two different stages of cells along the axis from pluripotent stem cells to fully differentiated NCs which are NPCs and differentiated NCs. Pluripotent NPCs can be obtained as disclosed herein and can be expanded to any suitable cell number, e.g., for a cell culture assay of a desirable format. It is possible to freeze and thaw healthy individuals and patients specific NPC aliquots. Accordingly, said NPCs can be expanded to a suitable cell number, frozen for storing or directly differentiated to produce robust differentiated NC culture assays according to the invention. Unexpectedly, the inventors found that using the specific conditions as disclosed in the present application differentiated primate NCs can be harvested and used as a source for cells with fixed neuronal identity. The differentiated primate NCs can be detached from the cell culture matrix at a late stage of differentiation without substantial loss of viability. The harvested differentiated primate NCs can be reseeded on a defined assay format, wherein the cells attach to the cell culture support and differentiation can be continued before or in parallel to applying a drug candidate to be tested. The method of the present invention solves the problem of non-uniformity of NC cultures from different primate species. Hence the primate NC cultures obtained with the method described herein are valuable models to screen effective and safe drugs and to elaborate new therapeutics for various diseases of the nervous system.

[0121] Accordingly, in a further aspect of the invention, the NC cultures produced according to the invention are used for testing the efficacy of at least one drug candidate. The drug candidate can be added to the cell culture medium at any stage of the method of the present invention. In one embodiment, the drug candidate is added to the differentiated NCs. In one embodiment, the drug candidate is added to the dissociated and reseeded differentiated NC to determine the efficacy profile of the drug candidate. In further embodiments, the drug candidate is added to the cell culture medium at about day 1, at about day 2, at about day 3, at about day 4, at about day 5, at about day 6, at about day 7, at about day 8, at about day 9, at about day 10, at about day 11, at about day 12, at about day 13, at about day 14, at about day 15, at about day 16, at about day 17, at about day 18, at about day 19, at about day 20, at about day 21, at about day 22, at about day 23, at about day 24, at about day 25, at about day 26, at about day 27, at about day 28, at about day 29, at about day 30, at about day 31, at about day 32, at about day 33, at about day 34, at about day 35, at about day 36, at about day 37, at about day 38, at about day 39 or at about day 40 of differentiation.

[0122] In a particular embodiment the step b)(i) comprises in this sequence dissociating the differentiated NCs from its support after about day 28 to about day 30 and step b)(ii) comprises reseeding the cells in a suitable cell culture format, continuing differentiation of the NCs for about 7 days, addition of a drug candidate to the cell culture medium, continuing differentiation of the NCs for about further 5 days and assessment of the efficacy profile of the drug candidate.

[0123] The primate NC cultures according to the present invention are characterised by uniform cell distribution and, therefore, testing the efficacy of novel drug candidates is straight-forward and well-standardized. The efficacy of a drug candidate can be determined by methods known to the art including but not limited to measuring a phenotypic marker, e.g., the expression of a marker, correlated to the efficacy of the drug candidate. In one embodiment the efficacy of a drug candidate is tested by determining the expression of a disease relevant marker. In one embodiment the efficacy of a drug candidate is tested by determining the expression of a disease relevant protein. In one embodiment the efficacy of a drug candidate is tested by determining the expression of a relevant marker by quantitative real time PCR. The determination of efficacy is performed at a defined time point after addition of a drug candidate. In further embodiments the determination of efficacy is performed at about day 1, at about day 2, at about day 3, at about day 4, at about day 5, at about day 6, at about day 7, at about day 8, at about day 9, at about day 10, at about day 11, at about day 12, at about day 13 or at about day 14 after addition of a drug candidate. In a particular embodiment the step b)(ii) comprises adding a drug candidate to the cell culture medium at about day 7 after dissociating and reseeding, and determining the efficacy of a drug candidate at about day 5 after addition of the drug candidate to the cell culture medium. Robust and uniform differentiated NC cultures according to the invention can be produced for different primate species and used to test the efficacy profile of drug candidates. In one embodiment, the method is suitable for inter-species comparison of efficacy between primate species, in particular between NHP species and human.

[0124] A further aspect of the invention is the use of the uniformly distributed differentiated primate NCs obtained by the methods as described herein. In a preferred embodiment the differentiated primate NCs obtained by the method of the present invention are used as in vitro model to study the pathophysiology of CNS diseases. For example, the differentiated primate NCs obtained by the method of the invention can be used for screening for compounds that reverse, inhibit or prevent neurological diseases. In one embodiment, the uniformly distributed differentiated primate NCs are used for screening for compounds that reverse, inhibit or prevent neural side effects of medicaments, for example diabetes medicaments.

[0125] In one embodiment, uniformly distributed differentiated primate NCs according to steps a) to b) are used for high-throughput screening of compounds and/or drug candidates selected from the group consisting of small molecules, proteins, peptides and nucleic acids. In a further embodiment, the differentiated NCs according to the invention are used for high-throughput screening of nucleic acid molecules such as a RNAi agent or an antisense oligonucleotide.

[0126] In a particular embodiment provided is an in vitro method for selecting at least one drug candidate for further development, comprising producing cell cultures of uniformly distributed differentiated neurons individually from human (Homo sapiens) and Cynomolgus monkey (Macaca fascicularis) comprising the steps of

[0127] a) providing neuronal precursor cells (NPCs) individually for both human and Cynomolgus monkey at a density of about 30000 cells/cm.sup.2 wherein the NPCs are derived from IPSCs;

[0128] b) differentiating the NPCs to neural cells (NCs) comprising the steps of

[0129] (i) incubating the NPCs with basal medium supplemented with Shh, FGF8 and ascorbic acid phosphate for about 7 days, replating the cells at a density of about 45000 cells/cm.sup.2, incubating the replated cells with basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid phosphate for about 21 days to about 23 days followed by dissociating the differentiated NCs from its support; and

[0130] (ii) individually reseeding the cells in a suitable cell culture format at a density of about 200000 cells/cm.sup.2 and continuing differentiation of the NCs for about 7 days by incubating the cells with basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid followed by incubating the cells with a drug candidate for about 5 days wherein the drug candidate is added to basal medium supplemented with BDNF, GDNF, cAMP and ascorbic acid; and

[0131] establishing the efficacy profiles of the drug candidate on both human and Cynomolgus monkey, and selecting drug candidates for further development if the efficacy profiles are favourable. In one embodiment, establishing the efficacy profile includes assessing target engagement. In one embodiment the further development comprises in vivo testing of the drug candidate in NHP species and/or in vivo testing in humans.

[0132] In one embodiment, a population of differentiated primate NCs produced by any of the foregoing methods is provided. In one embodiment, the differentiated primate NCs are dissociated and reseeded, and further differentiated to obtain uniform and standardized cultures of differentiated NCs. In one embodiment the primate NCs are derived from a healthy individual. In another embodiment, patient-derived primate NCs are used to generate a disease relevant in vitro model to study the pathophysiology of CNS diseases. Conversion of patient specific somatic cells to differentiated NCs represents an easy accessible and reproducible technology to generate a source of patient specific NCs for high-throughput cellular assays for disease modelling or compound screening.

[0133] In one embodiment, somatic cells from an Angelman syndrome patient are used to generate NPCs. The NPCs derived from one or several patients suffering from Angelman syndrome can be used to generate a disease model of Angelman syndrome. A human monogenic disease model can be recapitulated in NHP species by introduction of the etiologic gene mutation into the respective NHP genome by methods known to the art, e.g., by introducing the respective mutation into NHP NPCs.

[0134] In a further embodiment, data generated using the cell assays of the present invention is intended for research purposes with the aim of addressing neural diseases like neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, Amyotrophic lateral sclerosis (ALS/Lou Gehrig's Disease) stroke, and spinal cord injury or for therapy of said neurological diseases. Importantly, the present invention leads to consistent and reproducible cell culture assays. Indeed, a major drawback of former cell culture assays deriving from primate stem cells is non-homogenous distribution of differentiating cells among the surface of the cell culture area. The present invention solves this issue by introducing a dissociation and reseeding step which is, surprisingly, tolerated by the differentiating primate NCs, said cells remaining viable and suitable to seed cell culture assays at a defined and uniform cell density. A homogenous cell distribution among the surface of a given cell culture containment leads to improved and more reproducible cell culture assays. Accordingly, a method is provided to generate standardized primate NC culture assays, wherein the obtained differentiated NC cultures according to steps a) to b) are characterized by a homogenous cell distribution, evenly distributed cells in high-throughput plate wells, reduced formation of clusters and/or clumps and more equal cell distribution. Consequently, the resulting assays display increased robustness, increased homogeneity and decreased variation between assay replicates. In one embodiment, the cells are distributed uniformly over the cell culture area, in particular as assessed by cell nucleus staining.

[0135] One embodiment is the use of the standardized NC cultures obtained by the methods according to the invention to determine the efficacy of a drug candidate. In a further aspect of the invention the standardized primate NC culture are used for in vitro testing of toxicity of a drug candidate. In a further aspect of the invention the standardized primate NC cultures are used for in vitro testing of the efficacy of a drug candidate. The cultures can be derived from healthy individuals and/or from diseased individuals and results from the efficacy and/or toxicity integrated to predict disease and/or therapy relevant physiological effects of a drug candidate. In one embodiment, the in vitro efficacy profile of a drug candidate is assessed and drug candidates with favourable efficacy profile are selected for further development. Further development may comprise in vivo testing of the drug candidate in NHP species and/or in vivo testing in humans.

[0136] In a particular embodiment provided is a method for determining the in vitro efficacy profile of a drug candidate using standardized cell cultures of uniformly distributed differentiated neural cells (NCs) from at least two primate species, wherein the differentiated NC cultures are qualified for high throughput screening, the method comprising the steps of:

[0137] a) dissociating the differentiated NCs from its support after about 20 days to about 45 days of differentiation and reseeding the differentiated NCs in a high-throughput cell culture format;

[0138] b) incubating the reseeded NCs in a differentiation medium;

[0139] c) contacting the reseeded NCs with a drug candidate; and

[0140] d) determining the in vitro efficacy profile of the drug candidate.

[0141] Assessing efficacy data in vitro and/or in vivo on NHP species prior to in vivo testing in humans is conclusive since the genetic distance between NHP species and human is small. This is in contrast to, e.g., rodent species which are genetically more distant to humans compared to NHP species. The small genetic distance between NHP species and humans is especially important when assessing drug candidates targeting a human polynucleotide sequence or if the drug candidate itself comprises a polynucleotide with or close to a sequence derived from a human genome. In one embodiment provided, is a method as described herein, wherein the determined in vitro efficacy profile of the drug candidate is used for inter-species comparison of the efficacy profile of a drug candidate, wherein the cell cultures are produced individually from cells of at least two primate species, wherein essentially the same conditions are applied to the cultures for all primate species and wherein the efficacy profile is determined and compared for all primate species. In one embodiment, provided is a method for selecting a drug candidate for further development comprising the steps of: (i) determining the in vitro efficacy profile of the drug candidate for a first and a second species according to the method as described herein; and (ii) selecting the drug candidate for further development if the efficacy profile of the drug candidate is favourable. In one embodiment the genetic similarity of the protein-coding regions between the first and the second species is high. In one embodiment the genetic similarity of the protein-coding regions between the first and the second species is higher than between human (Homo sapiens) and mouse (Mus musculus). In further embodiments the genetic similarity of the protein-coding regions between the first and the second species is higher than 85%, higher than 90% or higher than 95%. In one embodiment the genetic similarity of the protein-coding regions between the first and the second species is higher than 90%. In one particular embodiment the first species is Cynomolgus monkey (Macaca fascicularis) and the second species is human (Homo sapiens). The differentiated NC cultures according to the invention may be produced sequentially for different species.

[0142] Accordingly, in one embodiment the standardized cell cultures of differentiated NCs from one or more primate species according to the present invention are used for in vitro efficacy testing of a drug candidate wherein the drug candidate comprises a polynucleotide or targets a specific sequence of a polynucleotide wherein the polynucleotide sequence derives from a human genome. In a further embodiment the drug candidate comprises nucleic acid molecules such as a RNAi agent or an antisense oligonucleotide.

[0143] In a further embodiment of the invention, the drug candidate assessed in the in vitro efficacy and/or toxicity tests comprises one or more antisense oligonucleotide. In further embodiments, the antisense oligonucleotide comprise or consist of 10 to 30 nucleotides in length with at least 90% identity, preferably 100% identity to a sequence derived from a human genome. It is understood that the antisense oligonucleotide sequences (motif sequence) can be modified to for example increase nuclease resistance and/or binding affinity to the target nucleic acid. In one aspect the antisense oligonucleotides comprise sugar-modified nucleosides and may also comprise DNA or RNA nucleosides. In some embodiments, the oligonucleotide comprise sugar-modified nucleosides and DNA nucleosides. In another aspect incorporation of modified nucleosides into the oligonucleotide enhance the affinity of the oligonucleotide for the target nucleic acid. In that case, the modified nucleosides can be referred to as affinity enhancing modified nucleotides.

[0144] In one embodiment, the antisense oligonucleotide comprises at least 1 modified nucleoside, such as at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15 or at least 16 modified nucleosides. In an embodiment the oligonucleotide comprises from 1 to 10 modified nucleosides, such as from 2 to 9 modified nucleosides, such as from 3 to 8 modified nucleosides, such as from 4 to 7 modified nucleosides, such as 6 or 7 modified nucleosides. In some embodiments, at least 1 of the modified nucleosides is a locked nucleic acid (LNA), such as at least 2, such as at least 3, at least 4, at least 5, at least 6, at least 7, or at least 8 of the modified nucleosides are LNA. In a still further embodiment all the modified nucleosides are LNA.

[0145] In one embodiment, the antisense oligonucleotide comprises modifications, which are independently selected from these three types of modifications (modified sugar, modified nucleobase and modified internucleoside linkage) or a combination thereof. Preferably the antisense oligonucleotide comprises one or more sugar modified nucleosides, such as 2' sugar modified nucleosides. Preferably the antisense oligonucleotide comprises the one or more 2' sugar modified nucleoside independently selected from the group consisting of 2'-O-alkyl-RNA, 2'-O-methyl-RNA, 2'-alkoxy-RNA, 2'-O-methoxyethyl-RNA, 2'-amino-DNA, 2'-fluoro-DNA, arabino nucleic acid (ANA), 2'-fluoro-ANA and LNA nucleosides. Even more preferably the one or more modified nucleoside is LNA.

[0146] In a further embodiment the antisense oligonucleotide comprises at least one modified internucleoside linkage. In a preferred embodiment the internucleoside linkages within the contiguous nucleotide sequence are phosphorothioate or boranophosphate internucleoside linkages.

[0147] In some embodiments, the antisense oligonucleotide comprise at least one modified nucleoside which is a 2'-MOE-RNA, such as 2, 3, 4, 5, 6, 7, 8, 9 or 10 2'-MOE-RNA nucleoside units. In some embodiments, at least one of said modified nucleoside is 2'-fluoro DNA, such as 2, 3, 4, 5, 6, 7, 8, 9 or 10 2'-fluoro-DNA nucleoside units.

[0148] In some embodiments, the oligonucleotide of the invention comprises at least one LNA unit, such as 1, 2, 3, 4, 5, 6, 7, or 8 LNA units, such as from 2 to 6 LNA units, such as from 3 to 7 LNA units, 4 to 8 LNA units or 3, 4, 5, 6 or 7 LNA units. In some embodiments, all the modified nucleosides are LNA nucleosides. In a further embodiment, the oligonucleotide may comprise both beta-D-oxy-LNA, and one or more of the following LNA units: thio-LNA, amino-LNA, oxy-LNA, and/or ENA in either the beta-D or alpha-L configurations or combinations thereof. In a further embodiment, all LNA cytosine units are 5-methyl-cytosine. In a preferred embodiment the oligonucleotide or contiguous nucleotide sequence has at least 1 LNA unit at the 5' end and at least 2 LNA units at the 3' end of the nucleotide sequence.

[0149] In some embodiments, the antisense oligonucleotide comprise at least one LNA unit and at least one 2' substituted modified nucleoside.

[0150] In some embodiments of the invention, the antisense oligonucleotide comprise both 2' sugar modified nucleosides and DNA units. Preferably the antisense oligonucleotide comprise both LNA and DNA units. Preferably, the combined total of LNA and DNA units is 8-30, such as 10-25, preferably 12-22, such as 12-18, even more preferably 11-16. In some embodiments of the invention, the nucleotide sequence of the antisense oligonucleotide, such as the contiguous nucleotide sequence consists of at least one or two LNA units and the remaining nucleotide units are DNA units. In some embodiments the antisense oligonucleotide comprises only LNA nucleosides and naturally occurring nucleosides (such as RNA or DNA, most preferably DNA nucleosides), optionally with modified internucleoside linkages such as phosphorothioate.

[0151] In an embodiment of the invention the antisense oligonucleotide is capable of recruiting RNase H.

[0152] In a preferred embodiment the antisense oligonucleotide has a gapmer design or structure also referred herein merely as "Gapmer". In a gapmer structure the antisense oligonucleotide comprises at least three distinct structural regions a 5'-flank, a gap and a 3'-flank, F-G-F' in `5->3` orientation. In this design, flanking regions F and F' (also termed wing regions) comprise a contiguous stretch of modified nucleosides, which are complementary to the target nucleic acid, while the gap region, G, comprises a contiguous stretch of nucleotides which are capable of recruiting a nuclease, preferably an endonuclease such as RNase, for example RNase H, when the antisense oligonucleotide is in duplex with the target nucleic acid. Nucleosides which are capable of recruiting a nuclease, in particular RNase H, can be selected from the group consisting of DNA, alpha-L-oxy-LNA, 2'-Flouro-ANA and UNA. Regions F and F', flanking the 5' and 3' ends of region G, preferably comprise non-nuclease recruiting nucleosides (nucleosides with a 3' endo structure), more preferably one or more affinity enhancing modified nucleosides.

[0153] In some embodiments, the 3' flank comprises at least one LNA nucleoside, preferably at least 2 LNA nucleosides. In some embodiments, the 5' flank comprises at least one LNA nucleoside. In some embodiments both the 5' and 3' flanking regions comprise a LNA nucleoside. In some embodiments all the nucleosides in the flanking regions are LNA nucleosides. In other embodiments, the flanking regions may comprise both LNA nucleosides and other nucleosides (mixed flanks), such as DNA nucleosides and/or non-LNA modified nucleosides, such as 2' substituted nucleosides. In this case the gap is defined as a contiguous sequence of at least 5 RNase H recruiting nucleosides (nucleosides with a 2' endo structure, preferably DNA) flanked at the 5' and 3' end by an affinity enhancing modified nucleoside, preferably LNA, such as beta-D-oxy-LNA. Consequently, the nucleosides of the 5' flanking region and the 3' flanking region which are adjacent to the gap region are modified nucleosides, preferably non-nuclease recruiting nucleosides.

[0154] In some embodiments, the modified nucleoside or the LNA nucleosides of the oligomer of the invention has a general structure of the formula I or II:

##STR00001##

[0155] wherein W is selected from --O--, --S--, --N(R.sup.a)--, --C(R.sup.aR.sup.b)--, such as, in some embodiments --O--;

[0156] B designates a nucleobase or modified nucleobase moiety;

[0157] Z designates an internucleoside linkage to an adjacent nucleoside, or a 5'-terminal group;

[0158] Z* designates an internucleoside linkage to an adjacent nucleoside, or a 3'-terminal group;

[0159] X designates a group selected from the list consisting of --C(R.sup.aR.sup.b)--, --C(R.sup.a).dbd.C(R.sup.b)--, --(R.sup.a).dbd.N--, --O--, --Si(R.sup.a).sub.2--, --S--, --SO.sub.2--, --N(R.sup.a)--, and >C.dbd.Z.

[0160] In some embodiments, X is selected from the group consisting of: --O--, --S--, NH--, NR.sup.aR.sup.b, --CH.sub.2--, CR.sup.aR.sup.b, --C(.dbd.CH.sub.2)--, and --C(.dbd.CR.sup.aR.sup.b)--.

[0161] In some embodiments, X is --O-- and Y designates a group selected from the group consisting of --C(R.sup.aR.sup.b)--, --C(R.sup.a).dbd.C(R.sup.b)--, --C(R.sup.a).dbd.N--, --O--, --Si(R.sup.a).sub.2--, --S--, --SO.sub.2--, --N(R.sup.a)--, and >C.dbd.Z.

[0162] In some embodiments, Y is selected from the group consisting of: --CH.sub.2--, --C(R.sup.aR.sup.b)--, --CH.sub.2CH.sub.2--, --C(R.sup.aR.sup.b)--C(R.sup.aR.sup.b)--, --CH.sub.2CH.sub.2CH.sub.2--, --C(R.sup.aR.sup.b)C(R.sup.aR.sup.b)C(R.sup.aR.sup.b)--, --C(R.sup.a).dbd.C(R.sup.b)--, and --C(R.sup.a).dbd.N--.

[0163] In some embodiments, Y is selected from the group consisting of: --CH.sub.2--, --CHR.sup.a--, --CHCH.sub.3--, CR.sup.aR.sup.b-- or --X--Y-- together designate a bivalent linker group (also referred to as a radicle) together designate a bivalent linker group consisting of 1, 2, 3 or 4 groups/atoms selected from the group consisting of --C(R.sup.aR.sup.b)--, --C(R.sup.a).dbd.C(R.sup.b)--, --C(R.sup.a).dbd.N--, --O--, --Si(R.sup.a).sub.2--, --S--, --SO.sub.2--, --N(R.sup.a)--, and >C.dbd.Z.

[0164] In some embodiments, --X--Y-- designates a biradicle selected from the groups consisting of: --X--CH.sub.2--, --X--CR.sup.aR.sup.b--, --X--CHR.sup.a--, --X--C(HCH.sub.3)--, --O--Y--, --O--CH.sub.2--, --S--CH.sub.2--, --NH--CH.sub.2--, --O--CHCH.sub.3--, --CH.sub.2--O--CH.sub.2, --O--CH(CH.sub.3CH.sub.3)--, --O--CH.sub.2--CH.sub.2--, OCH.sub.2--CH.sub.2--CH.sub.2--, --O--CH.sub.2OCH.sub.2--, --O--NCH.sub.2--, --C(.dbd.CH.sub.2)--CH.sub.2--, --NR.sup.a--CH.sub.2--, N--O--CH.sub.2, --S--CR.sup.aR.sup.b-- and --S--CHR.sup.a--.

[0165] In some embodiments --X--Y-- designates --O--CH.sub.2-- or --O--CH(CH.sub.3)-- wherein Z is selected from --O--, --S--, and --N(R.sup.a)--, and R.sup.a and, when present R.sup.b, each is independently selected from hydrogen, optionally substituted C.sub.1-6-alkyl, optionally substituted C.sub.2-6-alkenyl, optionally substituted C.sub.2-6-alkynyl, hydroxyl, optionally substituted C.sub.1-6-alkoxy, C.sub.2-6-alkoxyalkyl, C.sub.2-6-alkenyloxy, carboxy, C.sub.1-6-alkoxycarbonyl, C.sub.1-6-alkylcarbonyl, formyl, aryl, aryloxy-carbonyl, aryloxy, arylcarbonyl, heteroaryl, heteroaryloxy-carbonyl, heteroaryloxy, heteroarylcarbonyl, amino, mono- and di(C.sub.1-6-alkyl)amino, carbamoyl, mono- and di(C.sub.1-6-alkyl)-amino-carbonyl, amino-C.sub.1-6-alkyl-aminocarbonyl, mono- and di(C.sub.1-6-alkyl)amino-C.sub.1-6-alkyl-aminocarbonyl, C.sub.1-6-alkyl-carbonylamino, carbamido, C.sub.1-6-alkanoyloxy, sulphono, C.sub.1-6-alkylsulphonyloxy, nitro, azido, sulphanyl, C.sub.1-6-alkylthio, halogen, where aryl and heteroaryl may be optionally substituted and where two geminal substituents R.sup.a and R.sup.b together may designate optionally substituted methylene (.dbd.CH.sub.2), wherein for all chiral centers, asymmetric groups may be found in either R or S orientation, wherein R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are independently selected from the group consisting of: hydrogen, optionally substituted C.sub.1-6-alkyl, optionally substituted C.sub.2-6-alkenyl, optionally substituted C.sub.2-6-alkynyl, hydroxy, C.sub.1-6-alkoxy, C.sub.2-6-alkoxyalkyl, C.sub.2-6-alkenyloxy, carboxy, C.sub.1-6-alkoxycarbonyl, C.sub.1-6-alkylcarbonyl, formyl, aryl, aryloxy-carbonyl, aryloxy, arylcarbonyl, heteroaryl, heteroaryloxy-carbonyl, heteroaryloxy, heteroarylcarbonyl, amino, mono- and di(C.sub.1-6-alkylamino, carbamoyl, mono- and di(C.sub.1-6-alkyl)-amino-carbonyl, amino-C.sub.16-alkyl-aminocarbonyl, mono- and di(C.sub.1-6-alkylamino-C.sub.1-6-alkyl-aminocarbonyl, C.sub.1-6-alkyl-carbonylamino, carbamido, C.sub.1-6-alkanoyloxy, sulphono, C.sub.1-6-alkylsulphonyloxy, nitro, azido, sulphanyl, C.sub.1-6-alkylthio, halogen, where aryl and heteroaryl may be optionally substituted, and where two geminal substituents together may designate oxo, thioxo, imino, or optionally substituted methylene.

[0166] In some embodiments R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are independently selected from C.sub.1-6 alkyl, such as methyl, and hydrogen.

[0167] In some embodiments R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen.

[0168] In some embodiments R.sup.1, R.sup.2, R.sup.3, are all hydrogen, and either R.sup.5 and R.sup.5* is also hydrogen and the other of R.sup.5 and R.sup.5*is other than hydrogen, such as C.sub.1-6 alkyl such as methyl.

[0169] In some embodiments, R.sup.a is either hydrogen or methyl. In some embodiments, when present, R.sup.b is either hydrogen or methyl.

[0170] In some embodiments, one or both of R.sup.a and R.sup.b is hydrogen.

[0171] In some embodiments, one of R.sup.a and R.sup.b is hydrogen and the other is other than hydrogen. In some embodiments, one of R.sup.a and R.sup.b is methyl and the other is hydrogen.

[0172] In some embodiments, both of R.sup.a and R.sup.b are methyl.

[0173] In some embodiments, the biradicle --X--Y-- is --O--CH.sub.2--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are all hydrogen. Such LNA nucleosides are disclosed in WO99/014226, WO00/66604, WO98/039352 and WO2004/046160 which are all hereby incorporated by reference, and include what are commonly known as beta-D-oxy LNA and alpha-L-oxy LNA nucleosides.

[0174] In some embodiments, the biradicle --X--Y-- is --S--CH.sub.2--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are all hydrogen. Such thio LNA nucleosides are disclosed in WO99/014226 and WO2004/046160 which are hereby incorporated by reference.

[0175] In some embodiments, the biradicle --X--Y-- is --NH--CH.sub.2--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. Such amino LNA nucleosides are disclosed in WO99/014226 and WO2004/046160 which are hereby incorporated by reference.

[0176] In some embodiments, the biradicle --X--Y-- is --O--CH.sub.2--CH.sub.2-- or --O--CH.sub.2--CH.sub.2-- CH.sub.2--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are all hydrogen. Such LNA nucleosides are disclosed in WO00/047599 and Morita et al, Bioorganic & Med. Chem. Lett. 12 73-76, which are hereby incorporated by reference, and include what are commonly known as 2'-O-4'C-ethylene bridged nucleic acids (ENA).

[0177] In some embodiments, the biradicle --X--Y-- is --O--CH.sub.2--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, and one of R.sup.5 and R.sup.5* are hydrogen, and the other of R.sup.5 and R.sup.5* is other than hydrogen such as C.sub.1-6 alkyl, such as methyl. Such 5' substituted LNA nucleosides are disclosed in WO2007/134181 which is hereby incorporated by reference.

[0178] In some embodiments, the biradicle --X--Y-- is --O--CR.sup.aR.sup.b--, wherein one or both of R.sup.a and R.sup.b are other than hydrogen, such as methyl, W is O, and all of R.sup.1, R.sup.2, R.sup.3, and one of R.sup.5 and R.sup.5'' are hydrogen, and the other of R.sup.5 and R.sup.5* is other than hydrogen such as C.sub.1-6 alkyl, such as methyl.

[0179] Such bis modified LNA nucleosides are disclosed in WO2010/077578 which is hereby incorporated by reference.

[0180] In some embodiments, the biradicle --X--Y-- designate the bivalent linker group --O--CH(CH.sub.2OCH.sub.3)-(2' O-methoxyethyl bicyclic nucleic acid--Seth at al., 2010, J. Org. Chem. Vol 75(5) pp. 1569-81). In some embodiments, the biradicle --X--Y-- designate the bivalent linker group --O--CH(CH.sub.2CH.sub.3)-- (2'O-ethyl bicyclic nucleic acid--Seth at al., 2010, J. Org. Chem. Vol 75(5) pp. 1569-81). In some embodiments, the biradicle --X--Y-- is --O--CHR.sup.a--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. Such 6' substituted LNA nucleosides are disclosed in WO10036698 and WO07090071 which are both hereby incorporated by reference.

[0181] In some embodiments, the biradicle --X--Y-- is --O--CH(CH.sub.2OCH.sub.3)--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. Such LNA nucleosides are also known as cyclic MOEs in the art (cMOE) and are disclosed in WO07090071.

[0182] In some embodiments, the biradicle --X--Y-- designate the bivalent linker group --O--CH(CH.sub.3)-- in either the R- or S-configuration. In some embodiments, the biradicle --X--Y-- together designate the bivalent linker group --O--CH.sub.2--O--CH.sub.2-- (Seth at al., 2010, J. Org. Chem). In some embodiments, the biradicle --X--Y-- is --O--CH(CH.sub.3)--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. Such 6' methyl LNA nucleosides are also known as cET nucleosides in the art, and may be either (S)cET or (R)cET stereoisomers, as disclosed in WO07090071 (beta-D) and WO2010/036698 (alpha-L) which are both hereby incorporated by reference.

[0183] In some embodiments, the biradicle --X--Y-- is --O--CR.sup.aR.sup.b--, wherein in neither R.sup.a or R.sup.b is hydrogen, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are all hydrogen. In some embodiments, R.sup.a and R.sup.b are both methyl. Such 6' di-substituted LNA nucleosides are disclosed in WO 2009006478 which is hereby incorporated by reference.

[0184] In some embodiments, the biradicle --X--Y-- is --S--CHR.sup.a--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. Such 6' substituted thio LNA nucleosides are disclosed in WO11156202 which is hereby incorporated by reference. In some 6' substituted thio LNA embodiments R.sup.a is methyl.

[0185] In some embodiments, the biradicle --X--Y-- is --C(.dbd.CH.sub.2)--C(R.sup.aR.sup.b)--, such as --C(.dbd.CH.sub.2)--CH.sub.2--, or --C(.dbd.CH.sub.2)--CH(CH.sub.3)--W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are all hydrogen. Such vinyl carbo LNA nucleosides are disclosed in WO08154401 and WO09067647 which are both hereby incorporated by reference.

[0186] In some embodiments the biradicle --X--Y-- is --N(--OR.sup.a)--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5'' are all hydrogen. In some embodiments R.sup.a is C.sub.1-6 alkyl such as methyl. Such LNA nucleosides are also known as N substituted LNAs and are disclosed in WO2008/150729 which is hereby incorporated by reference. In some embodiments, the biradicle --X--Y-- together designate the bivalent linker group --O--NR.sup.a--CH.sub.3-- (Seth at al., 2010, J. Org. Chem). In some embodiments the biradicle --X--Y-- is --N(R.sup.a)--, W is O, and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. In some embodiments R.sup.a is C.sub.1-6 alkyl such as methyl.

[0187] In some embodiments, one or both of R.sup.5 and R.sup.5* is hydrogen and, when substituted the other of R.sup.5 and R.sup.5* is C.sub.1-6 alkyl such as methyl. In such an embodiment, R.sup.1, R.sup.2, R.sup.3, may all be hydrogen, and the biradicle --X--Y-- may be selected from --O--CH.sub.2-- or --O--C(HCR.sup.a)--, such as --O--C(HCH.sub.3)--.

[0188] In some embodiments, the biradicle is --CR.sup.aR.sup.b--O--CR.sup.aR.sup.b--, such as CH.sub.2--O--CH.sub.2--, W is O and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. In some embodiments R.sup.a is C.sub.1-6 alkyl such as methyl. Such LNA nucleosides are also known as conformationally restricted nucleotides (CRNs) and are disclosed in WO2013036868 which is hereby incorporated by reference.

[0189] In some embodiments, the biradicle is --O--CR.sup.aR.sup.b--O--CR.sup.aR.sup.b--, such as O--CH.sub.2--O--CH.sub.2--, W is O and all of R.sup.1, R.sup.2, R.sup.3, R.sup.5 and R.sup.5* are all hydrogen. In some embodiments R.sup.a is C.sub.1-6 alkyl such as methyl. Such LNA nucleosides are also known as COC nucleotides and are disclosed in Mitsuoka et al., Nucleic Acids Research 2009 37(4), 1225-1238, which is hereby incorporated by reference.

[0190] It will be recognized than, unless specified, the LNA nucleosides may be in the beta-D or alpha-L stereoisoform.

[0191] Further gapmer designs are disclosed in WO2004/046160, WO2007/146511 and incorporated by reference.

[0192] An aspect of the invention is to modulate the level of pig, primate or human UBE3A protein expression, in particular to increase the expression of paternal UBE3A expression in neuronal cells, in particular in human neuronal cells. The human UBE3A protein exists in several isoforms which are listed under Uniprot nr. Q05086. Several mutations in the maternal UBE3A gene can results in Angelman syndrome.

[0193] The target nucleic acid for the oligonucleotides of this aspect of the invention is RNA, in particular a long non-coding RNA. The long non-coding RNA which is targeted by the oligonucleotides of the present invention is human SNHG14 (also known as UBE3A-ATS with Ensembl entry number ENSG00000224078, version GRCh38.p2). In particular the target nucleic acid is the region downstream of SNORD109B corresponding to position 25278410 to 25419462 on chromosome 15 (SEQ ID NO: 1). In Rhesus monkey (Macaca mulatta) the UBE3A supressor is defined as region downstream of SNORD109A corresponding to position 4222848 to U.S. Pat. No. 4,373,084 (forward strand) on chromosome 7 using the Ensembl assembly MMUL 1.0 (SEQ ID NO: 2).

[0194] In some embodiments, the target nucleic acid is SEQ ID NO: 1, or naturally occurring variants thereof. In certain embodiments target nucleic acid correspond to regions which are conserved between human (SEQ ID NO: 1) and Rhesus monkey (SEQ ID NO: 2). In certain embodiments target nucleic acid correspond to regions which are conserved between human (SEQ ID NO:1), Rhesus monkey (SEQ ID NO: 2) and mouse (SEQ ID NO: 3).

[0195] In certain embodiments target nucleic acid is the region that is antisense to the UBE3A pre-mRNA, this region corresponds to position 55319 to 141053 of SEQ ID NO: 1.

[0196] In some embodiments, the target nucleic acid is present in a cell, such as a mammalian cell in particular a human cell in vitro or in vivo (the target cell). In certain embodiments the target cell is a neuron, preferably a human neuronal cell.

[0197] The target sequence may be a sub-sequence of the target nucleic acid. In some embodiments the oligonucleotide targets sub-sequence selected from the group consisting of the antisense region of exon 9, exon10, exon13, exon14, intron 14, exon 15, intron15 and exon 16 of UBE3A. In some embodiments the oligonucleotide or contiguous nucleotide sequence hybridize or is complementary to a single stranded nucleic acid molecule selected from the group consisting of positions: 55319-76274, 77483-77573, 92157-93403 and 97056-97354 of SEQ ID NO: 1. In some embodiments the oligonucleotide or contiguous nucleotide sequence hybridize or is complementary to a single stranded nucleic acid molecule selected from the group consisting of positions: 60821-60849, 77567-77583, 92323-92339 and 97156-97172 of SEQ ID NO: 1.

Particular Embodiments

[0198] 1. A method for determining the in vitro efficacy profile of a drug candidate using standardized cell cultures of uniformly distributed differentiated neural cells (NCs) from at least two primate species, wherein the differentiated NC cultures are qualified for high throughput screening, the method comprising the steps of:

[0199] a) dissociating the differentiated NCs from its support after about 20 days to about 45 days of differentiation and reseeding the differentiated NCs in a high-throughput cell culture format;

[0200] b) incubating the reseeded NCs in a differentiation medium;

[0201] c) contacting the reseeded NCs with the drug candidate; and

[0202] d) determining the in vitro efficacy profile of the drug candidate.

[0203] 2. The method according to embodiment 1, wherein the primate species are selected from the group consisting of human (Homo sapiens), Cynomolgus monkey (Macaca fascicularis) and Rhesus monkey (Macaca mulatta).

[0204] 3. The method according to embodiment 1, wherein one of the primate species is human (Homo sapiens).

[0205] 4. The method according to any one of embodiment 1 or 2, wherein one of the primate species is Cynomolgus monkey (Macaca fascicularis).

[0206] 5. The method of any one of embodiments 1 to 4, wherein the differentiated NCs are derived from induced pluripotent stem cells (iPSCs).

[0207] 6. The method according to any one of embodiments 1 to 5, wherein the differentiated NCs are uniformly distributed over the cell culture area as assessed by cell nucleus staining.

[0208] 7. The method according to any one of embodiments 1 to 6, wherein the distribution of the differentiated NCs is assessed by DNA staining, in particular by Hoechst staining.

[0209] 8. The method according to any one of embodiments 1 to 7, wherein step d) additionally comprises monitoring the cell cultures for signs of toxicity.

[0210] 9. The method according to any one of embodiments 1 to 8, wherein step d) comprises monitoring the cell cultures for a phenotypic change indicative of the efficacy of the drug candidate.

[0211] 10. The method according to any one of embodiments 1 to 9, wherein the determined in vitro efficacy profile of the drug candidate is used for inter-species comparison of the efficacy profile of a drug candidate, wherein the cell cultures are produced individually from cells of at least two primate species, wherein essentially the same conditions are applied to the cultures for all primate species and wherein the efficacy profile is determined and compared for all primate species.

[0212] 11. A method for selecting a drug candidate for further development comprising the steps of:

[0213] (i) determining the in vitro efficacy profile of the drug candidate for a first and a second species according to the method of any one of embodiments 1 to 10; and

[0214] (ii) selecting the drug candidate for further development if the efficacy profile of the drug candidate is favourable.

[0215] 12. The method according to embodiment 11 wherein the genetic similarity between the first and the second species is high, in particular more than 90%.

[0216] 13. The method according to any one of embodiments 11 or 12, wherein the first species is cynomolgus monkey (Macaca fascicularis) and the second species is human (Homo sapiens).

[0217] 14. The method according to any one of embodiments 11 to 13, wherein the drug candidate comprises a nucleic acid molecule or targets a specific nucleic acid sequence.

[0218] 15. The method according to any one of embodiments 11 to 14, wherein the drug candidate comprises at least one nucleic acid molecule such as a RNAi agent or an antisense oligonucleotide.

[0219] 16. The method according to any one of embodiments 11 to 15, wherein the further development comprises determining the in vivo efficacy and/or toxicity profile of the drug candidate.

[0220] 17. A method for determining the potential in vivo efficacy of a drug candidate wherein the in vitro efficacy profile of a drug candidate is determined according to any one of embodiments 1 to 10 and wherein the in vitro efficacy profile is indicative for in vivo efficacy.

[0221] 18. The method according to embodiment 17 wherein the in vitro efficacy profile of a drug candidate is indicative for in vivo efficacy in human (Homo sapiens).

[0222] 19. The method according to embodiment 17, wherein the in vitro efficacy profile of a drug candidate is indicative for in vivo efficacy in cynomolgus monkey (Macaca fascicularis).

[0223] 20. The method according to embodiment 17 to 19, wherein the in vivo efficacy profile is determined in at least one species.

[0224] 21. The method according to embodiment 17 to 20, wherein the in vivo efficacy profile is determined in cynomolgus monkey (Macaca fascicularis).

[0225] 25 22. The method according to any one of embodiments 17 to 21, wherein the determined in vitro efficacy profile and/or in vivo efficacy profile of the drug candidate is indicative for in vivo efficacy in human (Homo sapiens).

[0226] 23. The method according to embodiment 21, wherein the determined in vitro efficacy profile and the in vivo efficacy profile of the drug candidate as assessed in cynomolgus monkey is indicative for in vivo efficacy in human (Homo sapiens).

[0227] 24. The method according to any one of embodiments 1 to 23, wherein the differentiation medium is basal medium supplemented with 20 ng/ml BDNF, 10 ng/ml GDNF, 0.5 mM cAMP, and 100 .mu.M ascorbic acid phosphate.

[0228] 25. The method according to any one of embodiment 1 to 24, wherein the cell cultures are produced sequentially for different species.

[0229] 26. The methods and uses essentially as described herein.

[0230] Any of the embodiments as described herein may be used singly or in combination. The examples below explain the invention in more detail. The following preparations and examples are given to enable those skilled in the art to more clearly understand and to practice the present invention. The present invention, however, is not limited in scope by the exemplified embodiments, which are intended as illustrations of single aspects of the invention only, and methods which are functionally equivalent are within the scope of the invention. Indeed, various modifications of the invention in addition to those disclosed herein will become apparent to those skilled in the art from the foregoing description and accompanying figures. Such modifications are intended to fall within the scope of the appended claims.

EXAMPLES

[0231] Materials and Methods

TABLE-US-00001 TABLE 3 List of oligonucleotides or contiguous nucleobase sequences complementary to SEQ ID NO: 1 (motif sequences indicated by SEQ ID NO), oligonucleotide designs made from these, as well as specific oligonucleotide compounds (indicated by CMP ID NO) designed based on the motif sequence. Start Seq CMP SEQ ID ID NO Motif Design Compound ID NO .DELTA.G.degree. NO 1 4 AACTTCATCAATATTTCCC 3-13-3 AACttcatcaatatttCCC 4_1 -23.36 1677 4 AACTTCATCAATATTTCCC 2-15-2 AActtcatcaatatttcCC 4_2 -19.60 1677 5 ACTTCATCAATATTTCCC 3-12-3 ACTtcatcaatatttCCC 5_1 -23.80 1677 5 ACTTCATCAATATTTCCC 2-14-2 ACttcatcaatatttcCC 5_2 -20.24 1677 6 CAACTTCATCAATATTTCCC 2-14-4 CAacttcatcaatattTCCC 6_1 -25.64 1677 6 CAACTTCATCAATATTTCCC 2-16-2 CAacttcatcaatatttcCC 6_2 -22.28 1677 7 CAACTTCATCAATATTTCC 4-13-2 CAACttcatcaatatttCC 7_1 -21.47 1678 7 CAACTTCATCAATATTTCC 2-15-2 CAacttcatcaatatttCC 7_2 -19.46 1678 8 CCAACTTCATCAATATTTCC 3-14-3 CCAacttcatcaatattTCC 8_1 -25.64 1678 9 CCCAACTTCATCAATATTTC 4-14-2 CCCAacttcatcaatattTC 9_1 -25.64 1679 10 ACCCAACTTCATCAATATTT 2-16-2 ACccaacttcatcaatatTT 10_1 -20.05 1680 11 CCCAACTTCATCAATATTT 4-13-2 CCCAacttcatcaatatTT 11_1 -23.96 1680 11 CCCAACTTCATCAATATTT 2-15-2 CCcaacttcatcaatatTT 11_2 -20.28 1680 12 ACCCAACTTCATCAATATT 4-13-2 ACCCaacttcatcaataTT 12_1 -23.64 1681 12 ACCCAACTTCATCAATATT 2-15-2 ACccaacttcatcaataTT 12_2 -19.18 1681 13 CCCAACTTCATCAATATT 4-12-2 CCCAacttcatcaataTT 13_1 -23.09 1681 13 CCCAACTTCATCAATATT 2-14-2 CCcaacttcatcaataTT 13_2 -19.41 1681 14 TACCCAACTTCATCAATAT 2-15-2 TAcccaacttcatcaatAT 14_1 -19.31 1682 15 TACCCAACTTCATCAATA 2-14-2 TAcccaacttcatcaaTA 15_1 -19.14 1683 16 TTACCCAACTTCATCAATA 2-15-2 TTacccaacttcatcaaTA 16_1 -19.74 1683 17 TTTACCCAACTTCATCAAT 4-13-2 TTTAcccaacttcatcaAT 17_1 -21.68 1684 17 TTTACCCAACTTCATCAAT 2-15-2 TTtacccaacttcatcaAT 17_2 -19.22 1684 18 ATACTTTACCCAACTTCAT 3-13-3 ATActttacccaacttCAT 18_1 -23.44 1688 18 ATACTTTACCCAACTTCAT 2-15-2 ATactttacccaacttcAT 18_2 -20.13 1688 19 TACTTTACCCAACTTCAT 3-12-3 TACtttacccaacttCAT 19_1 -22.78 1688 19 TACTTTACCCAACTTCAT 2-14-2 TActttacccaacttcAT 19_2 -19.30 1688 20 TTATACTTTACCCAACTTCA 2-16-2 TTatactttacccaacttCA 20_1 -21.40 1689 21 TCACTGTTCTGACTTT 3-10-3 TCActgttctgacTTT 21_1 -19.11 1712 22 TTCAATCTCTATCTCATCAT 2-16-2 TTcaatctctatctcatcAT 22_1 -19.42 4169 23 CTTCAATCTCTATCTCATCA 4-14-2 CTTCaatctctatctcatCA 23_1 -24.21 4170 23 CTTCAATCTCTATCTCATCA 2-16-2 CTtcaatctctatctcatCA 23_2 -22.04 4170 24 TTCAATCTCTATCTCATCA 2-15-2 TTcaatctctatctcatCA 24_1 -19.44 4170 25 CTTCAATCTCTATCTCATC 2-15-2 CTtcaatctctatctcaTC 25_1 -19.87 4171 26 ACTTCAATCTCTATCTCAT 3-13-3 ACTtcaatctctatctCAT 26_1 -22.36 4172 26 ACTTCAATCTCTATCTCAT 2-15-2 ACttcaatctctatctcAT 26_2 -19.08 4172 27 CACTTCAATCTCTATCTCAT 2-16-2 CActtcaatctctatctcAT 27_1 -20.98 4172 28 ACTTCAATCTCTATCTCA 2-12-4 ACttcaatctctatCTCA 28_1 -21.96 4173 28 ACTTCAATCTCTATCTCA 2-14-2 ACttcaatctctatctCA 28_2 -19.10 4173 29 CACTTCAATCTCTATCTCA 2-13-4 CActtcaatctctatCTCA 29_1 -23.86 4173 29 CACTTCAATCTCTATCTCA 2-15-2 CActtcaatctctatctCA 29_2 -21.00 4173 30 ACACTTCAATCTCTATCTC 2-15-2 ACacttcaatctctatcTC 30_1 -19.38 4174 31 TACACTTCAATCTCTATCTC 2-14-4 TAcacttcaatctctaTCTC 31_1 -23.31 4174 31 TACACTTCAATCTCTATCTC 2-16-2 TAcacttcaatctctatcTC 31_2 -20.53 4174 32 TACACTTCAATCTCTATCT 4-13-2 TACActtcaatctctatCT 32_1 -22.34 4175 33 CTTTGTCTCTCTTTACT 2-13-2 CTttgtctctctttaCT 33_1 -19.36 4374 34 TATACCTTTCTTTAACCC 3-12-3 TATacctttctttaaCCC 34_1 -24.89 8118 34 TATACCTTTCTTTAACCC 2-14-2 TAtacctttctttaacCC 34_2 -20.83 8118 35 TGTTTATACCCTTTCC 2-12-2 TGtttataccctttCC 35_1 -20.33 9212 36 TCTCCTTTATGACTCC 2-10-4 TCtcctttatgaCTCC 36_1 -23.29 10839 37 CTTCTCCTTTATGACTC 2-13-2 CTtctcctttatgacTC 37_1 -19.26 10840 38 CCATTTATTTCCATTTATT 4-13-2 CCATttatttccatttaTT 38_1 -22.32 15567 38 CCATTTATTTCCATTTATT 2-15-2 CCatttatttccatttaTT 38_2 -19.61 15567 39 CTTTCCATTTATTTCCATTT 2-14-4 CTttccatttatttccATTT 39_1 -23.14 15570 40 TCTTTCCATTTATTTCCATT 2-14-4 TCtttccatttatttcCATT 40_1 -24.62 15571 41 ATTACCCATCCGTTCT 2-12-2 ATtacccatccgttCT 41_1 -21.15 21965 42 GCATTAGGCACATTACAT 3-12-3 GCAttaggcacattaCAT 42_1 -23.96 22211 43 ATTATTATTTAACCTTCCTA 2-16-2 ATtattatttaaccttccTA 43_1 -19.28 30451 44 ACATTATTATTTAACCTTCC 4-14-2 ACATtattatttaaccttCC 44_1 -22.84 30453 44 ACATTATTATTTAACCTTCC 2-16-2 ACattattatttaaccttCC 44_2 -20.13 30453 45 CATTATTATTTAACCTTCC 4-13-2 CATTattatttaaccttCC 45_1 -22.04 30453 45 CATTATTATTTAACCTTCC 2-15-2 CAttattatttaaccttCC 45_2 -19.55 30453 46 CCTCTGCTTATAACTTT 2-13-2 CCtctgcttataactTT 46_1 -19.15 30699 47 CTACTATACTTTCCTCT 2-11-4 CTactatactttcCTCT 47_1 -22.32 30711 48 GTTCTACTATACTTTCC 4-11-2 GTTCtactatactttCC 48_1 -21.69 30714 48 GTTCTACTATACTTTCC 2-13-2 GTtctactatactttCC 48_2 -19.21 30714 49 CACCTGATAACAGACCCT 3-12-3 CACctgataacagacCCT 49_1 -26.38 36068 50 CACCTGATAACAGACC 3-10-3 CACctgataacagACC 50_1 -21.10 36070 51 CCCACCAAAGGATATATT 3-12-3 CCCaccaaaggatatATT 51_1 -23.47 37208 52 ACCAGCTACAGGAACCTC 3-12-3 ACCagctacaggaacCTC 52_1 -26.57 46132 53 CTATATCTCACTCCTATTT 4-13-2 CTATatctcactcctatTT 53_1 -23.07 48143 53 CTATATCTCACTCCTATTT 2-13-4 CTatatctcactcctATTT 53_2 -22.12 48143 54 CTATATCTCACTCCTATT 2-14-2 CTatatctcactcctaTT 54_1 -19.40 48144 54 CTATATCTCACTCCTATT 2-12-4 CTatatctcactccTATT 54_2 -22.28 48144 54 CTATATCTCACTCCTATT 3-12-3 CTAtatctcactcctATT 54_3 -21.44 48144 55 CTACTATATCTCACTCCTAT 2-16-2 CTactatatctcactcctAT 55_1 -22.00 48145 55 CTACTATATCTCACTCCTAT 2-14-4 CTactatatctcactcCTAT 55_2 -25.54 48145 56 TACTATATCTCACTCCTAT 2-13-4 TActatatctcactcCTAT 56_1 -23.29 48145 57 CTACTATATCTCACTCCTA 2-15-2 CTactatatctcactccTA 57_1 -21.91 48146 58 TACTATATCTCACTCCTA 2-14-2 TActatatctcactccTA 58_1 -19.66 48146 58 TACTATATCTCACTCCTA 2-12-4 TActatatctcactCCTA 58_2 -23.59 48146 58 TACTATATCTCACTCCTA 3-12-3 TACtatatctcactcCTA 58_3 -22.62 48146 59 CTACTATATCTCACTCCT 2-14-2 CTactatatctcactcCT 59_1 -21.25 48147 59 CTACTATATCTCACTCCT 4-12-2 CTACtatatctcactcCT 59_2 -23.87 48147 60 CTACTATATCTCACTCC 2-13-2 CTactatatctcactCC 60_1 -20.13 48148 60 CTACTATATCTCACTCC 2-11-4 CTactatatctcaCTCC 60_2 -23.00 48148 60 CTACTATATCTCACTCC 3-11-3 CTActatatctcacTCC 60_3 -22.56 48148 61 CCTACTATATCTCACTC 2-11-4 CCtactatatctcACTC 61_1 -21.93 48149 62 CTCCTACTATATCTCACTC 4-13-2 CTCCtactatatctcacTC 62_1 -25.69 48149 63 TCCTACTATATCTCACTC 3-12-3 TCCtactatatctcaCTC 63_1 -23.88 48149 64 CTCCTACTATATCTCACT 4-12-2 CTCCtactatatctcaCT 64_1 -24.87 48150 64 CTCCTACTATATCTCACT 3-12-3 CTCctactatatctcACT 64_2 -22.93 48150 65 TTTCCTCTCCTACTATATC 2-15-2 TTtcctctcctactataTC 65_1 -21.23 48155 66 ATCCATATCCTTTCCT 3-10-3 ATCcatatcctttCCT 66_1 -24.02 48168 67 CATCCATATCCTTTCCT 4-11-2 CATCcatatcctttcCT 67_1 -24.94 48168 68 ATCATCCATATCCTTTCC 4-12-2 ATCAtccatatcctttCC 68_1 -25.69 48169 69 CATCATCCATATCCTTTC 4-12-2 CATCatccatatccttTC 69_1 -23.32 48170 69 CATCATCCATATCCTTTC 2-14-2 CAtcatccatatccttTC 69_2 -20.72 48170 69 CATCATCCATATCCTTTC 2-12-4 CAtcatccatatccTTTC 69_3 -22.56 48170 70 TACATCATCCATATCCTTTC 2-16-2 TAcatcatccatatccttTC 70_1 -22.45 48170 70 TACATCATCCATATCCTTTC 4-14-2 TACAtcatccatatccttTC 70_2 -25.00 48170 70 TACATCATCCATATCCTTTC 2-14-4 TAcatcatccatatccTTTC 70_3 -24.29 48170 71 ACATCATCCATATCCTTT 3-12-3 ACAtcatccatatccTTT 71_1 -22.11 48171 72 CATCATCCATATCCTTT 2-13-2 CAtcatccatatcctTT 72_1 -19.04 48171 72 CATCATCCATATCCTTT 4-11-2 CATCatccatatcctTT 72_2 -21.64 48171 73 TACATCATCCATATCCTTT 2-15-2 TAcatcatccatatcctTT 73_1 -20.76 48171 73 TACATCATCCATATCCTTT 2-13-4 TAcatcatccatatcCTTT 73_2 -23.36 48171 73 TACATCATCCATATCCTTT 3-13-3 TACatcatccatatccTTT 73_3 -22.88 48171 74 ATACATCATCCATATCCTT 2-15-2 ATacatcatccatatccTT 74_1 -20.80 48172 74 ATACATCATCCATATCCTT 4-13-2 ATACatcatccatatccTT 74_2 -23.12 48172 75 TACATCATCCATATCCTT 2-14-2 TAcatcatccatatccTT 75_1 -19.97 48172 75 TACATCATCCATATCCTT 4-12-2 TACAtcatccatatccTT 75_2 -22.52 48172 76 TATACATCATCCATATCCTT 2-16-2 TAtacatcatccatatccTT 76_1 -21.36 48172 77 ATACATCATCCATATCCT 3-12-3 ATAcatcatccatatCCT 77_1 -24.15 48173 77 ATACATCATCCATATCCT 2-14-2 ATacatcatccatatcCT 77_2 -20.55 48173 77 ATACATCATCCATATCCT 2-13-3 ATacatcatccatatCCT 77_3 -22.92 48173 78 ATATACATCATCCATATCCT 2-16-2 ATatacatcatccatatcCT 78_1 -22.04 48173 79 TACATCATCCATATCCT 2-11-4 TAcatcatccataTCCT 79_1 -23.21 48173 79 TACATCATCCATATCCT 2-13-2 TAcatcatccatatcCT 79_2 -19.71 48173 79 TACATCATCCATATCCT 4-11-2 TACAtcatccatatcCT 79_3 -22.27 48173

80 TATACATCATCCATATCCT 2-15-2 TAtacatcatccatatcCT 80_1 -21.11 48173 80 TATACATCATCCATATCCT 3-13-3 TATacatcatccatatCCT 80_2 -25.15 48173 80 TATACATCATCCATATCCT 4-13-2 TATAcatcatccatatcCT 80_3 -24.01 48173 81 ATACATCATCCATATCC 3-11-3 ATAcatcatccataTCC 81_1 -21.79 48174 82 ATATACATCATCCATATCC 4-13-2 ATATacatcatccatatCC 82_1 -23.73 48174 82 ATATACATCATCCATATCC 2-15-2 ATatacatcatccatatCC 82_2 -20.93 48174 83 TATACATCATCCATATCC 2-14-2 TAtacatcatccatatCC 83_1 -20.00 48174 83 TATACATCATCCATATCC 4-12-2 TATAcatcatccatatCC 83_2 -22.90 48174 84 TATATACATCATCCATATCC 2-16-2 TAtatacatcatccatatCC 84_1 -21.49 48174 84 TATATACATCATCCATATCC 4-14-2 TATAtacatcatccatatCC 84_2 -24.29 48174 85 GCTTCATATTTCTCCA 2-12-2 GCttcatatttctcCA 85_1 -20.44 49345 86 CATCTTGTTCTTTACCT 2-13-2 CAtcttgttctttacCT 86_1 -19.67 49581 87 TATATTCACCATTGCC 2-10-4 TAtattcaccatTGCC 87_1 -22.70 49724 88 CCTTATATTCACCATTG 2-13-2 CCttatattcaccatTG 88_1 -19.44 49726 88 CCTTATATTCACCATTG 2-11-4 CCttatattcaccATTG 88_2 -21.25 49726 89 CCTCCTTATATTCACC 4-10-2 CCTCcttatattcaCC 89_1 -24.64 49730 90 CCCTTCCTTTATTCAA 3-10-3 CCCttcctttattCAA 90_1 -23.86 50189 91 CCTTACTGTTAAATCCT 2-13-2 CCttactgttaaatcCT 91_1 -19.81 50475 92 CAGGCAGATAACCTCCAA 3-12-3 CAGgcagataacctcCAA 92_1 -25.31 52419 93 CAGCAGGCAGATAACCTC 3-12-3 CAGcaggcagataacCTC 93_1 -25.88 52422 94 CGAATCTTGACATACAGG 3-12-3 CGAatcttgacatacAGG 94_1 -21.47 53955 95 CTCATACTTGCTTTAAT 4-11-2 CTCAtacttgctttaAT 95_1 -19.10 60821 95 CTCATACTTGCTTTAAT 2-13-2 CTcatacttgctttaAT 95_2 -16.3 60821 96 ACATCTCATACTTGCTT 2-11-4 ACatctcatacttGCTT 96_1 -21.31 60825 96 ACATCTCATACTTGCTT 2-13-2 ACatctcatacttgcTT 96_2 -17.66 60825 96 ACATCTCATACTTGCTT 2-12-3 ACatctcatacttgCTT 96_3 -19.52 60825 97 ACATCTCATACTTGCT 2-10-4 ACatctcatactTGCT 97_1 -21.18 60826 97 ACATCTCATACTTGCT 2-12-2 ACatctcatacttgCT 97_2 -17.70 60826 97 ACATCTCATACTTGCT 2-11-3 ACatctcatacttGCT 97_3 -19.49 60826 97 ACATCTCATACTTGCT 4-10-2 ACATctcatacttgCT 97_4 -20.48 60826 98 TACATCTCATACTTGCT 2-11-4 TAcatctcatactTGCT 98_1 -22.33 60826 98 TACATCTCATACTTGCT 2-13-2 TAcatctcatacttgCT 98_2 -18.85 60826 98 TACATCTCATACTTGCT 4-11-2 TACAtctcatacttgCT 98_3 -21.40 60826 99 CCTACATCTCATACTTGC 3-12-3 CCTacatctcatactTGC 99_1 -26.29 60827 99 CCTACATCTCATACTTGC 2-14-2 CCtacatctcatacttGC 99_2 -22.98 60827 99 CCTACATCTCATACTTGC 2-13-3 CCtacatctcatactTGC 99_3 -24.67 60827 99 CCTACATCTCATACTTGC 2-12-4 CCtacatctcatacTTGC 99_4 -25.70 60827 100 CTACATCTCATACTTGC 3-11-3 CTAcatctcatactTGC 100_1 -22.33 60827 100 CTACATCTCATACTTGC 2-13-2 CTacatctcatacttGC 100_2 -19.41 60827 100 CTACATCTCATACTTGC 2-12-3 CTacatctcatactTGC 100_3 -21.10 60827 101 TACATCTCATACTTGC 3-10-3 TACatctcatactTGC 101_1 -19.94 60827 101 TACATCTCATACTTGC 2-12-2 TAcatctcatacttGC 101_2 -17.15 60827 101 TACATCTCATACTTGC 2-11-3 TAcatctcatactTGC 101_3 -18.85 60827 101 TACATCTCATACTTGC 4-10-2 TACAtctcatacttGC 101_4 -19.71 60827 102 CCTACATCTCATACTTG 4-11-2 CCTAcatctcatactTG 102_1 -22.52 60828 102 CCTACATCTCATACTTG 2-13-2 CCtacatctcatactTG 102_2 -19.67 60828 102 CCTACATCTCATACTTG 3-12-2 CCTacatctcatactTG 102_3 -21.29 60828 102 CCTACATCTCATACTTG 3-11-3 CCTacatctcatacTTG 102_4 -22.31 60828 103 ACCTACATCTCATACTT 3-11-3 ACCtacatctcataCTT 103_1 -21.93 60829 103 ACCTACATCTCATACTT 2-13-2 ACctacatctcatacTT 103_2 -17.76 60829 103 ACCTACATCTCATACTT 2-11-4 ACctacatctcatACTT 103_3 -20.03 60829 103 ACCTACATCTCATACTT 3-12-2 ACCtacatctcatacTT 103_4 -20.26 60829 104 CCTACATCTCATACTT 3-10-3 CCTacatctcataCTT 104_1 -21.50 60829 104 CCTACATCTCATACTT 2-12-2 CCtacatctcatacTT 104_2 -18.21 60829 104 CCTACATCTCATACTT 2-10-4 CCtacatctcatACTT 104_3 -20.48 60829 105 TACCTACATCTCATACTT 4-12-2 TACCtacatctcatacTT 105_1 -22.49 60829 105 TACCTACATCTCATACTT 2-14-2 TAcctacatctcatacTT 105_2 -18.81 60829 105 TACCTACATCTCATACTT 2-13-3 TAcctacatctcataCTT 105_3 -20.48 60829 105 TACCTACATCTCATACTT 2-12-4 TAcctacatctcatACTT 105_4 -21.08 60829 106 TTACCTACATCTCATACTT 3-13-3 TTAcctacatctcataCTT 106_1 -22.30 60829 106 TTACCTACATCTCATACTT 2-15-2 TTacctacatctcatacTT 106_2 -19.40 60829 106 TTACCTACATCTCATACTT 2-14-3 TTacctacatctcataCTT 106_3 -21.08 60829 106 TTACCTACATCTCATACTT 2-13-4 TTacctacatctcatACTT 106_4 -21.67 60829 107 ACCTACATCTCATACT 4-10-2 ACCTacatctcataCT 107_1 -21.72 60830 107 ACCTACATCTCATACT 2-12-2 ACctacatctcataCT 107_2 -17.61 60830 107 ACCTACATCTCATACT 3-11-2 ACCtacatctcataCT 107_3 -20.10 60830 107 ACCTACATCTCATACT 2-10-4 ACctacatctcaTACT 107_4 -20.11 60830 108 TACCTACATCTCATACT 4-11-2 TACCtacatctcataCT 108_1 -22.34 60830 108 TACCTACATCTCATACT 2-13-2 TAcctacatctcataCT 108_2 -18.66 60830 108 TACCTACATCTCATACT 3-12-2 TACctacatctcataCT 108_3 -19.85 60830 108 TACCTACATCTCATACT 3-11-3 TACctacatctcatACT 108_4 -20.44 60830 109 TTACCTACATCTCATACT 2-12-4 TTacctacatctcaTACT 109_1 -21.75 60830 109 TTACCTACATCTCATACT 2-14-2 TTacctacatctcataCT 109_2 -19.25 60830 109 TTACCTACATCTCATACT 3-13-2 TTAcctacatctcataCT 109_3 -20.48 60830 109 TTACCTACATCTCATACT 3-12-3 TTAcctacatctcatACT 109_4 -21.08 60830 110 TTACCTACATCTCATAC 3-11-3 TTAcctacatctcaTAC 110_1 -19.50 60831 110 TTACCTACATCTCATAC 2-13-2 TTacctacatctcatAC 110_2 -16.37 60831 111 GTTACCTACATCTCATA 2-11-4 GTtacctacatctCATA 111_1 -21.69 60832 111 GTTACCTACATCTCATA 2-13-2 GTtacctacatctcaTA 111_2 -18.74 60832 111 GTTACCTACATCTCATA 3-12-2 GTTacctacatctcaTA 111_3 -19.98 60832 112 GTTACCTACATCTCAT 3-10-3 GTTacctacatctCAT 112_1 -20.69 60833 112 GTTACCTACATCTCAT 2-12-2 GTtacctacatctcAT 112_2 -17.37 60833 113 ATATACCCAAAGGCACCT 3-12-3 ATAtacccaaaggcaCCT 113_1 -25.99 62200 114 TCTACTCATCCTTTAACTCA 2-14-4 TCtactcatcctttaaCTCA 114_1 -25.63 62251 115 CCTTAATCTGTATCACT 2-13-2 CCttaatctgtatcaCT 115_1 -19.58 62286 116 CCATACACAGCACATA 2-12-2 CCatacacagcacaTA 116_1 -19.04 62424 117 CTCCATACACAGCACAT 2-13-2 CTccatacacagcacAT 117_1 -20.08 62425 118 CAGAATAATTCTCCTCC 2-13-2 CAgaataattctcctCC 118_1 -19.86 62441 119 GTCCTACATATATACC 4-10-2 GTCCtacatatataCC 119_1 -22.09 66380 120 TGCTTCCTTACTAACC 4-10-2 TGCTtccttactaaCC 120_1 -23.93 66701 120 TGCTTCCTTACTAACC 2-12-2 TGcttccttactaaCC 120_2 -20.10 66701 121 CCCTTTGTAATCATCT 4-10-2 CCCTttgtaatcatCT 121_1 -23.44 66838 122 TCCCTTTGTAATCATCT 2-13-2 TCcctttgtaatcatCT 122_1 -19.97 66838 123 CTGCCATCAATACCAT 2-12-2 CTgccatcaataccAT 123_1 -19.14 68918 124 TCACTGCCATCAATACC 2-13-2 TCactgccatcaataCC 124_1 -21.35 68920 125 ATTCTTACTTTATTCCTCA 2-15-2 ATtcttactttattcctCA 125_1 -20.16 70033 126 TCACTTTCCAGATATCA 4-11-2 TCACtttccagatatCA 126_1 -21.61 77567 126 TCACTTTCCAGATATCA 2-13-2 TCactttccagatatCA 126_2 -18.65 77567 127 TCCTTCAAATTCCACATAC 3-13-3 TCCttcaaattccacaTAC 127_1 -24.09 82053 128 ACATGTCCCTTTATATT 4-11-2 ACATgtccctttataTT 128_1 -20.87 92323 128 ACATGTCCCTTTATATT 2-13-2 ACatgtccctttataTT 128_2 -17.66 92323 128 ACATGTCCCTTTATATT 3-12-2 ACAtgtccctttataTT 128_3 -19.13 92323 128 ACATGTCCCTTTATATT 3-11-3 ACAtgtccctttatATT 128_4 -20.03 92323 129 ACATGTCCCTTTATAT 3-10-3 ACAtgtccctttaTAT 129_1 -20.11 92324 129 ACATGTCCCTTTATAT 2-12-2 ACatgtccctttatAT 129_2 -16.74 92324 130 CCAAGAAAGGAGCAAGCT 3-12-3 CCAagaaaggagcaaGCT 130_1 -25.26 97146 131 TCCAAGAAAGGAGCAAGC 3-12-3 TCCaagaaaggagcaAGC 131_1 -24.12 97147 132 CTCATCCCTCCAAGAAA 4-11-2 CTCAtccctccaagaAA 132_1 -22.58 97156 132 CTCATCCCTCCAAGAAA 2-13-2 CTcatccctccaagaAA 132_2 -19.83 97156 132 CTCATCCCTCCAAGAAA 3-12-2 CTCatccctccaagaAA 132_3 -21.11 97156 133 TCATCCCTCCAAGAAA 4-10-2 TCATccctccaagaAA 133_1 -20.41 97156 133 TCATCCCTCCAAGAAA 2-12-2 TCatccctccaagaAA 133_2 -17.63 97156 133 TCATCCCTCCAAGAAA 3-11-2 TCAtccctccaagaAA 133_3 -19.09 97156 133 TCATCCCTCCAAGAAA 3-10-3 TCAtccctccaagAAA 133_4 -19.81 97156 134 CACCTCCCTATTACATAAA 4-13-2 CACCtccctattacataAA 134_1 -24.18 100018 134 CACCTCCCTATTACATAAA 2-15-2 CAcctccctattacataAA 134_2 -20.51 100018 135 CACCTCCCTATTACATAA 4-12-2 CACCtccctattacatAA 135_1 -23.75 100019 135 CACCTCCCTATTACATAA 2-14-2 CAcctccctattacatAA 135_2 -20.07 100019 136 CCTCCCTATTACATAA 2-12-2 CCtccctattacatAA 136_1 -18.40 100019 137 CTAAATCTTCCAATTCATA 2-15-2 CTaaatcttccaattcaTA 137_1 -18.12 106139 138 TATCCCTTGATTATCCT 2-13-2 TAtcccttgattatcCT 138_1 -20.68 109406 139 CCTCTTTGTCAAATACT 2-13-2 CCtctttgtcaaataCT 139_1 -19.30 110768 140 CAGCTTATTTACCTCTT 2-13-2 CAgcttatttacctcTT 140_1 -19.30 114828 141 ACTCTTTACCTCTAACACT 4-13-2 ACTCtttacctctaacaCT 141_1 -24.26 117468 142 TTACTCTTTACCTCTAACAC 3-14-3 TTActctttacctctaaCAC 142_1 -23.23 117469

143 CCAACCTAATACCTTAATA 2-15-2 CCaacctaataccttaaTA 143_1 -20.27 118639 144 TACCAACCTAATACCTTAA 2-15-2 TAccaacctaataccttAA 144_1 -18.32 118641 145 CCAATACCCACAAACC 3-10-3 CCAatacccacaaACC 145_1 -23.17 124162 145 CCAATACCCACAAACC 2-12-2 CCaatacccacaaaCC 145_2 -20.85 124162 146 CCATTATTCTACTTTGT 3-11-3 CCAttattctacttTGT 146_1 -21.79 125501 146 CCATTATTCTACTTTGT 2-13-2 CCattattctactttGT 146_2 -18.63 125501 147 CATTTCCTTATCTTCACA 2-14-2 CAtttccttatcttcaCA 147_1 -20.39 125529 148 TCATTTCCTTATCTTCACA 4-13-2 TCATttccttatcttcaCA 148_1 -24.13 125529 149 AATAATTCCTCATTTCCT 2-14-2 AAtaattcctcatttcCT 149_1 -18.01 125539 150 ACAATAATTCCTCATTTCC 3-13-3 ACAataattcctcattTCC 150_1 -22.71 125540 150 ACAATAATTCCTCATTTCC 2-15-2 ACaataattcctcatttCC 150_2 -20.23 125540

[0232] Designs refer to the gapmer design, F-G-F', where each number represents the number of consecutive modified nucleosides, e.g 2' modified nucleosides (first number=5' flank), followed by the number of DNA nucleosides (second number=gap region), followed by the number of modified nucleosides, e.g. 2' modified nucleosides (third number=3' flank), optionally preceded by or followed by further repeated regions of DNA and LNA, which are not necessarily part of the contiguous sequence that is complementary to the target nucleic acid.

[0233] For the oligonucleotide compounds capital letters represent beta-D-oxy LNA nucleosides, lowercase letters represent DNA nucleosides, all LNA C are 5-methyl cytosine, and 5-methyl DNA cytosines are presented by "e", all internucleoside linkages are phosphorothioate internucleoside linkages

[0234] Oligonucleotide Synthesis

[0235] Oligonucleotide synthesis is generally known in the art. Below is a protocol which may be applied. The oligonucleotides of the present invention may have been produced by slightly varying methods in terms of apparatus, support and concentrations used.

[0236] Oligonucleotides are synthesized on uridine universal supports using the phosphoramidite approach on a MerMade12 or an Oligomaker DNA/RNA synthesizer at 1-4 .mu.mol scale. At the end of the synthesis, the oligonucleotides are cleaved from the solid support using aqueous ammonia for 5-16 hours at 60.degree. C. The oligonucleotides are purified by reverse phase HPLC (RP-HPLC) or by solid phase extractions and characterized by UPLC, and the molecular mass is further confirmed by ESI-MS.

[0237] Elongation of the Oligonucleotide:

[0238] The coupling of .beta.-cyanoethyl-phosphoramidites (DNA-A(Bz), DNA-G(ibu), DNA-C(Bz), DNA-T, LNA-5-methyl-C(Bz), LNA-A(Bz), LNA-G(dmf), LNA-T or amino-C6 linker) is performed by using a solution of 0.1 M of the 5'-O-DMT-protected amidite in acetonitrile and DCI (4,5-dicyanoimidazole) in acetonitrile (0.25 M) as activator. For the final cycle a phosphoramidite with desired modifications can be used, e.g. a C6 linker for attaching a conjugate group or a conjugate group as such. Thiolation for introduction of phosphorthioate linkages is carried out by using xanthane hydride (0.01 M in acetonitrile/pyridine 9:1). Phosphordiester linkages can be introduced using 0.02 M iodine in THF/Pyridine/water 7:2:1. The rest of the reagents are the ones typically used for oligonucleotide synthesis.

[0239] Purification by RP-HPLC:

[0240] The crude compounds are purified by preparative RP-HPLC on a Phenomenex Jupiter C18 10.mu. 150.times.10 mm column. 0.1 M ammonium acetate pH 8 and acetonitrile is used as buffers at a flow rate of 5 mL/min. The collected fractions are lyophilized to give the purified compound typically as a white solid.

Abbreviations

[0241] DCI: 4,5-Dicyanoimidazole

[0242] DCM: Dichloromethane

[0243] DMF: Dimethylformamide

[0244] DMT: 4,4'-Dimethoxytrityl

[0245] THF: Tetrahydrofurane

[0246] Bz: Benzoyl

[0247] Ibu: Isobutyryl

[0248] RP-HPLC: Reverse phase high performance liquid chromatography

[0249] T.sub.m Assay

[0250] Oligonucleotide and RNA target duplexes are diluted to 3 mM in 500 ml RNase-free water and mixed with 500 ml 2.times. T.sub.m-buffer (200 mM NaCl, 0.2 mM EDTA, 20 mM Naphosphate, pH 7.0). The solution is heated to 95.degree. C. for 3 min and then allowed to anneal in room temperature for 30 min. The duplex melting temperatures (T.sub.m) is measured on a Lambda 40 UV/VIS Spectrophotometer equipped with a Peltier temperature programmer PTP6 using PE Templab software (Perkin Elmer). The temperature is ramped up from 20.degree. C. to 95.degree. C. and then down to 25.degree. C., recording absorption at 260 nm. First derivative and the local maximums of both the melting and annealing are used to assess the duplex T.sub.m.

[0251] Preparation of Mouse Primary Cortical Neuron Cell Cultures

[0252] Primary cortical neuron cultures were prepared from mouse embryo brains of 15 days of age according to standard procedure. In brief, culture plates were coated with Poly-L-Lysine (50 .mu.g/ml Poly-L-Lysine, 10 mM Na-tetraborate, pH 8 buffer) for 2-3 hrs at room temperature. The plates were washed with 1.times.PBS before use. Harvested mouse embryo brains were dissected and homogenized by a razor blade and submerged into 38 ml dissection medium (HBSS, 0.01 M Hepes, Penicillin/Streptomycin). Then, 2 ml trypsin was added and cells were incubated for 30 min at 37.degree. C. and centrifuged down. The cells were dissolved in 20 ml DMEM (+10% FBS) and passed through a syringe for further homogenization. This was followed by centrifugation at 500 rpm for 15 mins. The cells were dissolved in DMEM (+10% FBS) and seeded in 96 well plates (0.1.times.10 6 cells/well in 100 .mu.l). The neuronal cell cultures were ready for use directly after seeding.

[0253] Screening Oligonucleotides in Mouse Primary Cortical Neuron Cell Cultures

[0254] Cells were cultured in growth medium (Gibco Neurobasal medium, B27 supplement, Glutamax, Pencillin-streptomycin) in 96-well plates and incubated with oligonucleotides for 3 days at the desired concentrations. Total RNA was isolated from the cells and the knock-down efficacy was measured by qPCR analysis using the gScript.TM. XLT One-Step RT-qPCR ToughMix.RTM., Low ROX.TM. kit from Quanta Bioscience (95134-500). A commercial taqman assays from Thermo Fisher Scientific was used to measure Ube3a_ATS including GAPDH for normalization.

[0255] Generation of Human Primary Neuronal Cell Cultures

[0256] Any cell lines at any described time point was incubated at 37.degree. C., 5% CO2 concentration and 95% relative humidity.

[0257] Human Induced Pluripotent Stem Cells (hIPSC) Culture

[0258] Whole human blood samples were obtained from patients diagnosed with Angelman syndrome. The subsequent cultures of primary Peripheral Blood Mononuclear Cells (PBMCs) were enriched for erythroblasts. Patient-specific IPSC lines were generated by reprogramming erythroblast with CytoTune-iPS Sendai Reprogramming Kit (Thermo Fisher Scientific). Derived IPSC lines were maintained in feeder-free conditions using hESC-qualified Matrigel (Corning) in mTESR1 (STEMCELL Technologies) with daily medium replacement. Upon reaching confluence, colonies were dissociated into cell cluster of 50-200 .mu.m in size using Gentle Cell Dissociation Reagent (STEMCELL Technologies) and subcultured at a ratio of 1:10-1:20 in the presence of 10 .mu.M Y-27632 (Calbiochem).

[0259] Differentiation into Neural Progenitor Cells (NPC)

[0260] Upon induction of neural differentiation IPSC-derived cells were maintained in basal medium composed of equal volumes of DMEM:F12 Glutamax medium and Neurobasal medium (Gibco, Invitrogen), supplemented with 1.times. B27 (Gibco, Invitrogen), 1.times. N2 (Gibco, Invitrogen), 0.1 mM beta-mercaptoethanol (Gibco, Invitrogen) and indicated supplements.

[0261] Neural progenitor cells (NPCs) were derived from hIPSCs by dual SMAD inhibition and according to published procedures with slight modifications (Chambers et al. 2009 Nat Biotechnol. Vol. 3 pp. 275-80, Boissart et al., 2013 Transl Psychiatry. 3:e294). HIPSCs were dissociated with Accutase (Innovative Cell Technologies Inc.) into a single cell suspension and resuspended in MT medium further supplemented with 10 .mu.M Y-27632 (Calbiochem), 5 ng/ml FGF (Peprotech), 10 .mu.M SB-431542 (Calbiochem) and 100 nM LDN (Calbiochem). Single cell suspension was transferred to AggreWell800 plates (STEMCELL Technologies) enabling the formation of aggregates consisting of 8000 cells. After 5 days neural aggregates were transferred onto plates coated with poly-L-ornithine (Sigma) and laminin (Roche) and allowed to form neural rosettes under continued dual SMAD inhibition (SB-431542 and LDN) in basal medium supplemented with FGF. Neural rosettes were selectively isolated using STEMdiff.TM. Neural Rosette Selection Reagent (STEMCELL Technologies), replated onto dishes coated with poly-L-ornithine and Laminin521 (BioLamina) and expanded in basal medium supplemented with 10 ng/ml FGF (Peprotech), 10 ng/ml EGF (RnD), and 20 ng/ml BDNF (Peprotech). When reaching confluency, cells were enzymatically dissociated with 0.05% Trypsin/EDTA (Gibco, Invitrogen) and sub-cultured. Continued passaging in basal medium supplemented with FGF, EGF and BDNF leads to a stable neural progenitor cell line (NPC line) within 10 to 20 passages. A stable neural progenitor cell line is defined by its capacity to self-renew and by the expression of the developmental stage-specific markers Sox2 and Nestin. Upon specific stimuli, NPCs differentiate into neuronal (MAP2+, Tau+, HuC/D+) and astroglial (GFAP+) progenies (Dunkley et al., 2015 Proteomics Clin Appl. Vol. 7-8 pp. 684-94).

[0262] NPC Culture

[0263] Conditions for NPC culture have been described previously and were used with slight modifications (Boissart et al., 2013 Transl Psychiatry. 3:e294). In brief, cells were maintained in dishes coated with Laminin521 (BioLamina) and cultured in basal medium [composed of equal volumes of DMEM:F12 Glutamax medium and Neurobasal medium (Gibco, Invitrogen), supplemented with 1.times. B27 (Gibco, Invitrogen), 1.times. N2 (Gibco, Invitrogen), 0.1 mM beta-mercaptoethanol (Gibco, Invitrogen)] and supplemented with 10 ng/ml FGF (Peprotech), 10 ng/ml EGF (RnD), and 20 ng/ml BDNF (Peprotech).

[0264] Differentiation into Neuronal Cell Culture

[0265] To induce neuronal differentiation of NPC, cells were dissociated with 0.05% Trypsin/EDTA (Gibco, Invitrogen) into single cell suspension and seeded onto Laminin521 (BioLamina) coated dishes at a density of 12.000 cells/cm2 and maintained in basal medium supplemented with 200 ng/ml Shh (Peprotech), 100 ng/ml FGF8 (Peprotech), and 100 .mu.M ascorbic acid phosphate (Sigma) for a period of 7 days. Subsequently, cells were replated in basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science), and 100 .mu.M ascorbic acid phosphate (Sigma) at a density of 45000 cells/cm2 and differentiated for a period of 21 days. At day 21 of differentiation, differentiated neuronal cultures were replated onto the screening-compatible plate format. Replating was performed by dissociating the cultures with Accutase (Innovative Cell Technologies Inc.) into a single cell suspension. Cells were seeded at a density of 200.000 cells/cm2 in presence of 10 .mu.M Y-27632 (a cell-permeable, reversible, inhibitor of Rho kinases from Calbiochem) into the 384 well microtiter plates for final oligonucleotides screening assay. Neuronal cultures were further differentiated for additional 7 days in basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science), and 100 .mu.M ascorbic acid phosphate (Sigma). Differentiation medium was exchanged twice per week. After a total differentiation period of 35 days neuronal cell cultures were ready for oligonucleotide treatment.

[0266] Screening Oligonucleotides in Human Neuronal Cell Cultures

[0267] For screening, oligonucleotide stocks were pre-diluted to the indicated concentrations with water into 384 well microtiter plates (compound plate). The plate layout served as a treatment template. Two microliter oligonucleotide dilution from each well was transferred from the compound plate to a respective culture plate. All liquid handling was done under sterile conditions in a laminar flow using a semi-automated laboratory robotic system (Beckmancoulter). Neuronal cell cultures were incubated with oligonucleotides for 5 days without media change. Subsequently, neuronal cultures were lysed and processed for qPCR assay with RealTime ready Cell lysis and RNA Virus Master kit (Roche). Liquid handling was performed using a semi-automated laboratory robotic system (Beckmancoulter). Samples were analyzed by a Lightcycler480 real-time PCR system (Roche).

[0268] Activity of the oligonucleotides was assessed by qPCR monitoring transcript abundance of UBE3A using the following primers and probes

TABLE-US-00002 UBE3a-Sense: Forward primer: ATATGTGGAAGCCGGAATCT, Reverse primer: TCCCAGAACTCCCTAATCAGAA, Internal probe labeled with dye FAM: ATGACGGTGGCTATACCAGG

[0269] The RT-qPCR was multiplexed with PPIA (peptidylprolyl isomerase A) as housekeeping gene for normalization. PPIA primers and probe labeled with the dye VIC were purchased from Thermo Fisher Scientific (assay ID Hs99999904_m1). Each plate includes a non-targeting oligonucleotide (mock) as negative control (TTGaataagtggaTGT) and a reference oligonucleotide CMP ID NO: 41_1, resulting in up-regulation of UBE3A mRNA. Selectivity of oligonucleotides was verified by counter screening for SNORD 115 transcript, which is located upstream of SNORD109B on chromosome 15. Expression of SNORD115 was monitored by qPCR using the following primers and probe

TABLE-US-00003 Forward primer: GGGTCAATGATGAGAACCTTAT, Reverse primer: GGGCCTCAGCGTAATCCTATT, Internal probe labeled with the dye FAM: TTCTGAAGAGAGGTGATGACTTAAAA

[0270] The RT-qPCR was multiplexed with PPIA (Thermo Fisher Scientific) upon oligonucleotide treatment.

[0271] The reduction of the SNHG14 transcript downstream of SNORD109B (also termed the UBE3A suppressor) was measured by RT-qPCR using the following primers and probe

TABLE-US-00004 Forward primer: ATCCGAGGCATGAATCTCAC, Reverse primer: CAGGCCAAAACCCTTGATAA, Internal probe labeled with dye FAM: TTGCTGAGCATTTTTGCATC)

[0272] The RT-qPCR was multiplexed with PPIA (Thermo Fisher Scientific).

[0273] Data are presented as average % expression relative to mock across all plates and normalized to the reference oligonucleotide to account for plate to plate variation

[0274] Screening Oligonucleotides in Human Neuronal Cell Cultures--96 Well System

[0275] For screening, oligonucleotide stocks were pre-diluted to the indicated concentrations with water into 96 well microtiter plates (compound plate). The plate layout served as a treatment template. Two microliter oligonucleotide dilution from each well was transferred from the compound plate to a respective culture plate. All liquid handling was done under sterile conditions in a laminar flow using a semi-automated laboratory robotic system (Beckman Coulter). Neuronal cell cultures were incubated with oligonucleotides for 5 days without media change. Subsequently, neuronal cultures were lysed and RNA purified using RNA purification kit Pure Link Pro96 (12173011A) LifeTechnologies. Liquid handling was performed using a semi-automated laboratory robotic system (Beckmancoulter). qPCR analysis of Ube3a and Ube3a-ATS was carried out on a ViiA.TM. 7 Real-Time PCR System Thermo Fisher Scientific using the qScript.TM. XLT 1-Step RT-qPCR ToughMix Low ROX, from Quanta (95134-50). The following primers and probes were used:

[0276] qPCR UBE3a-Sense:

TABLE-US-00005 Forward primer: ATATGTGGAAGCCGGAATCT, Reverse primer: TCCCAGAACTCCCTAATCAGAA, Internal probe labeled with dye FAM: ATGACGGTGGCTATACCAGG

[0277] qPCR SNHG14 transcript downstream of SNORD109B (also termed the UBE3A suppressor): Commercially available primer and probe set from Thermo Fisher: Hs01372957_m1. These primers amplifies a 87 bp exon-exon spanning sequence in the Genbank transcript AF400500.1

[0278] Qpcr Gapdh Transcript:

[0279] Commercially available primer and probe set from Thermo Fisher: Gene Symbol: with following assay details: RefSeq: NM_002046.3, Probe Exon Location:3, Amplicon Size: 122 bp. Corresponding TaqMan Assay ID: Hs99999905_m1.

[0280] The RT-qPCR for both Ube3a and Ube3a-ATS was multiplexed with GAPDH as housekeeping gene for normalization. Each plate includes a non-targeting oligonucleotide (mock) as negative control (TTGaataagtggaTGT) and a reference oligonucleotide CMP ID NO: 21_1, resulting in up-regulation of UBE3A mRNA. Moreover panel of control oligonucleotides not targeting Ub3a or SNHG14 transcript downstream of SNORD109B (also termed the UBE3A suppressor) were included to monitor the assay noise and risk of detecting false positives. These were randomly distributed over the plates.

[0281] Control Oligonucleotides:

TABLE-US-00006 SEQUENCE CGAaccactgaaCAA CGAaccactgaacAAA CGAagtgcacaCG GCGtaaagagaGGT GAGAaggcacagaCGG GCGaagtgcacaCGG GAGaaggcacagaCGG CGAaccactgAACA GAAccactgaacAAA caGCGtaaagagaGG GCgtaaagagAGG CGAaccactgaAC CGAAccactgaaCAAA AGCgaagtgcacaCGG AGGtgaagcgaAGTG TAGTaaactgagCCA AGAaggcacagaCGG CCGcagtatggaTCG

[0282] Generation of Cynomolgus Primary Neuronal Cell Cultures

[0283] Any cell lines at any described time point was incubated at 37.degree. C., 5% CO2 concentration and 95% relative humidity.

[0284] Cynomolgus Induced Pluripotent Stem Cells (cIPSC) Culture

[0285] All animal procedures were performed in accordance with the guidelines of the National Institutes of Health and approved by the Institutional Animal Care and Use Committee (IACUC) affiliated with Roche 340 Kingsland Street Nutley N.J. 07110, USA (closed 2014). Cynomolgus IPSCs were established from kidney fibroblasts from a female, 14-year-old Mauritian Cynomolgus monkey using Sendai virus particles (CytoTune-iPS Sendai Reprogramming Kit, Thermo Fisher Scientific) harboring the Yamanaka factors (Oct4, Sox2, Klf4, and C-Myc) (Takahashi, K. & Yamanaka, S., 2006). Five days post-transfection, cells were passed onto mitomycin C inactivated feeders at varying densities and cultured in hESC media (knock-out DMEM:F12 supplemented with 20% knock-out serum replacement, 0.1 mM non-essential amino acids, 2 mM L-glutamine, 0.1 mM 2-mercaptoethanol, and 8 ng/ml bFGF, all from Life Technologies). Twenty days post-transfection clones with ES-like morphologies were selected for further passaging. The cells were routinely passaged every 3-4 days by manual dissociation of colonies. After at least 10 passages, cIPSCs were adapted to feeder-free culture conditions (MT medium supplemented with 15 ng/ml FGF2 (Peprotech) and 10 ng/ml ActivinA (Peprotech)) based on Ono et al.; 2014 with the modification that cells were cultured on Matrigel (BD Bioscience)-coated plates and that Y-27632 (Calbiochem) was used instead of Thiazovivin when the cells were in single cell suspension. MT medium is a defined medium that contains Dulbecco's Modified Eagle Medium with Ham's F12 Nutrient Mixture (DMEM/F12) with 2.5 mM GlutaMAX.TM., 7 .mu.g/ml insulin, 450 .mu.M monothioglycerole, 1.times. Lipid concentrate, 5 mg/ml BSA, 14 ng/ml sodium selenite, 1.times. non-essential amino acids, 2 mg/ml heparin, 15 .mu.g/ml transferrin, and 220 .mu.M ascorbic acid-2-phosphate. The cells were passaged every 2-4 days using Gentle Cell Dissociation Reagent (Stem Cell Technologies). One clone was chosen for further experiments and used for all the following assays.

[0286] Differentiation into Neural Progenitor Cells (NPC)

[0287] Upon induction of neural differentiation cIPSC-derived cells (see FIG. 4: day 1) were maintained in basal medium composed of equal volumes of DMEM:F12 Glutamax medium and Neurobasal medium (Gibco, Invitrogen), supplemented with 1.times. B27 (Gibco, Invitrogen), 1.times. N2 (Gibco, Invitrogen), 0.1 mM beta-mercaptoethanol (Gibco, Invitrogen) and indicated supplements.

[0288] Neural progenitor cells (NPCs) were derived from cIPSCs by dual SMAD inhibition and according to published procedures with slight modifications (Chambers et al. 2009 Nat Biotechnol. Vol. 3 pp. 275-80, Boissart et al., 2013 Transl Psychiatry. 3:e294). CynolPSCs were dissociated with Accutase (Innovative Cell Technologies Inc.) into a single cell suspension and resuspended in MT medium supplemented with 10 .mu.M Y-27632 (Calbiochem). Single cell suspension was transferred to AggreWell800 plates (STEMCELL Technologies) enabling the formation of aggregates consisting of 12000 cells (see FIG. 4: EB formation). For the next five consecutive days 1.5 ml of medium per AggreWell was replaced by basal medium supplemented with 5 ng/ml FGF (Peprotech), 10 .mu.M SB-431542 (Calbiochem) and 100 nM LDN-193189 (Calbiochem). After 5 days neural aggregates were transferred onto plates coated with poly-L-ornithine (Sigma) and laminin (Roche) and allowed to form neural rosettes under continued dual SMAD inhibition (SB-431542 and LDN-193189) in basal medium supplemented with FGF (see FIG. 4: Harvesting). Neural rosettes were selectively isolated by manual dissection, thereafter replated onto dishes coated with poly-L-ornithine and Laminin521 from BioLamina (see FIG. 4: neural rosette isolation) and expanded in basal medium supplemented with 10 ng/ml FGF (Peprotech), 10 ng/ml EGF (RnD), and 20 ng/ml BDNF (Peprotech). When reaching confluency, cells were enzymatically dissociated with 0.05% Trypsin/EDTA (Gibco, Invitrogen) and sub-cultured. Continued passaging in basal medium supplemented with FGF, EGF and BDNF leads to a stable neural progenitor cell line (NPC line) within 5 to 10 passages (see FIG. 4, day 24-45). A stable neural progenitor cell line is defined by its capacity to self-renew and by the expression of the developmental stage-specific markers Sox2 and Nestin. Upon specific stimuli, cyno NPCs differentiate into neuronal MAP2+ cells.

[0289] NPC Culture

[0290] Conditions for NPC culture have been described previously and were used with slight modifications (Boissart et al., 2013 Transl Psychiatry. 3:e294). In brief, cells were maintained in dishes coated with Laminin521 (BioLamina) and cultured in basal medium [composed of equal volumes of DMEM:F12 Glutamax medium and Neurobasal medium (Gibco, Invitrogen), supplemented with 1.times. B27 (Gibco, Invitrogen), 1.times. N2 (Gibco, Invitrogen), 0.1 mM beta-mercaptoethanol (Gibco, Invitrogen)] supplemented with 10 ng/ml FGF (Peprotech), 10 ng/ml EGF (RnD), and 20 ng/ml BDNF (Peprotech).

[0291] Differentiation into Neuronal Cell Culture

[0292] To induce neuronal differentiation of NPC, cells were dissociated with 0.05% Trypsin/EDTA (Gibco, Invitrogen) into single cell suspension and seeded onto Laminin521 (BioLamina) coated dishes at a density of 30.000 cells/cm2 and maintained in basal medium supplemented with 200 ng/ml Shh (Peprotech), 100 ng/ml FGF8 (Peprotech), and 100 .mu.M ascorbic acid phosphate (Sigma) for a period of 7 days. Subsequently, cells were replated in basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science), and 100 .mu.M ascorbic acid phosphate (Sigma) at a density of 45000 cells/cm2 and differentiated for a period of 21 days. At day 21 of differentiation, differentiated NCs were dissociated and reseeded onto the screening-compatible plate format. Replating was performed by dissociating the cultures with Accutase (Innovative Cell Technologies Inc.) into a single cell suspension. Cells were seeded at a density of 200000 cells/cm2 in presence of 10 .mu.M Y-27632 (a cell-permeable, reversible, inhibitor of Rho kinases from Calbiochem) into the 384 well microtiter plates for final oligonucleotides screening assay. Neuronal cultures were further differentiated for additional 7 days in basal medium supplemented with 20 ng/ml BDNF (Peprotech), 10 ng/ml GDNF (Peprotech), 0.5 mM cAMP (BIOLOG Life Science) and 100 .mu.M ascorbic acid phosphate (Sigma). Differentiation medium was exchanged twice per week. After a total differentiation period of 35 days neuronal cell cultures were ready for oligonucleotide treatment.

[0293] Screening Oligonucleotides in Cyno Neuronal Cell Cultures--96 Well System

[0294] For screening, oligonucleotide stocks were pre-diluted to the indicated concentrations with water into 96 well microtiter plates (compound plate). The plate layout served as a treatment template. Two microliter oligonucleotide dilution from each well was transferred from the compound plate to a respective culture plate. All liquid handling was done under sterile conditions in a laminar flow using a semi-automated laboratory robotic system (Beckmancoulter). Neuronal cell cultures were incubated with oligonucleotides for 5 days without media change. Subsequently, neuronal cultures were lysed and purified using PureLink.RTM. Pro 96 total RNA Purification Kit (Thermo Fisher) and thereafter processed for qPCR assay with qScript XLT One-Step RT-qPCR Tough Mix, Low ROX (Quanta Biosiences). Liquid handling was performed using a semi-automated laboratory robotic system (Integra Assist and Integra Viaflo). Samples were analyzed by a Lightcycler480 real-time PCR system (Roche).

[0295] Activity of the oligonucleotides was assessed by qPCR monitoring transcript abundance of UBE3A-Sense and UBE3A-Antisense using the following primers and probes:

[0296] UBE3a-Sense: HS00166580_VIC Taqman assay (Thermo Fisher)

[0297] UBE3A-Antisense: HS01372957_VIC Taqman assay (Thermo Fisher)

[0298] The RT-qPCR was multiplexed with TBP (TATA-Box Binding Protein) as housekeeping gene for normalization. TBP primers and FAM labeled probe were purchased from Roche (REF.:05532957001, Assay Id. 101145). Each plate includes a non-targeting oligonucleotide (mock) as negative control (TTGaataagtggaTGT).

[0299] Data are presented as average percentage expression relative to mock treated conditions.

[0300] Immunocytofluorescence Staining

[0301] Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX (Sigma) in PBS (with Ca2+ and Mg2+). Blocking was performed using SuperBlock solution (Thermo Fisher Scientific) supplemented with 0.1% TritonX. Cells were stained with the following primary antibodies: anti-SOX2 (Milipore AB5603), anti-GFAP (Dako Z033401), anti-HuC/D (Invitrogen A21271), anti-Nestin (Millipore mab5326) and anti-Map2 (Neuromics CH22103). Subsequently, the cells were washed and stained with secondary antibodies conjugated either to Alexa488, Alexa555 or Alexa647 (all Molecular Probes). Nuclei were stained with Hoechst 1:1000 (Molecular Probes). The cells were imaged using an Axiovert microscope (Zeiss) or an Operetta high content imaging system (Perkin Elmer). Images were analyzed using ImageJ software.

[0302] qPCR Analysis

[0303] RNA from cIPSC, cyno differentiated NCs, hESCs and human differentiated NCs was isolated using the miRNeasy Mini kit (QIAGEN) and qPCR analysis was performed (for human cells using the Ag-Path-ID One-Step RT-PCR kit, Ambion; for cyno cells reverse transcription was performed using the Transcriptor First Strand cDNA synthesis kit, Roche and qPCR using LightCycler 480 Probes Master, Roche). The following primers and probes were used: for human cells: NES Hs04187831_g1; MAP2 Hs00258900_m1; ASCL1 Hs00269932_m1; SOX2 Hs01053049_s1; ELAVL3 Hs00154959_m1; GAPDH 4352665, all from Thermo Fisher/Applied Biosystems; UBE3A-ATS Fw primer: CAA ATG CCT CAC CCA CTC TT, RV primer: CCA GCT GTC AAC ATG TGC TT, internal oligo: AAG TGC GCT CCT GTG AAA AG; UBE3A Fw primer: TCT GGG AAA TCG TT CATT CA, RV primer: TGT AGG TAA CCT TTC TGT GTC TGG, internal oligo TAC AAC GGG CAC AGA CAG AG. For cyno cells: NANOG Assay ID 700103, POU5F1 Assay ID 113034, SOX2 Assay ID 111867, NESTIN Assay ID 138150, PAX6 Assay ID 136139, SOX1 Assay ID 136988, ZIC1 Assay ID 112077, ASCL1 Assay ID 700027, TUBB3 Assay ID 700047, GAPDH Cat N. 04694333001, Probe #147; TBP Assay ID 101145, all purchased from Roche. UBE3A-ATS Fw primer: AATGCAAAGGCAGCAGTACA, Rv primer: TTGGGGAGTTGGTTATTGGA, internal oligo TGACACCACCAGAAGAACACA;

TABLE-US-00007 UBE3A sense: Fw primer: ATATGTGGAAGCCGGAATCT, Rv primer: TCCCAGAACTCCCTAATCAGAA, internal oligo: ATGACGGTGGCTATACCAGG.

[0304] RNA Sequencing Analysis

[0305] RNA isolation was performed using the miRNeasy Mini kit (QIAGEN). Library preparation was performed using the TruSeq Stranded Total RNA LT kit (illumina) with Ribo-Zero Gold depletion. Cluster generation was performed using the cBOT instrument and the HiSeq PE Cluster kit v4. Sequencing was performed on the HiSeq 2500 instrument as paired-end Single-indexed Sequencing run (2.times.125 cycles) with the following reagents: HiSeq SBS Kit v4 250 cycles (illumina).

[0306] Western Blot Analysis

[0307] For protein expression studies, the cells were collected by scraping and flash frozen. The pellets were lysed using Cytobuster (Millipore) completed with phosphatase and protease inhibitors and DNAse (all from Roche). The protein concentration in the extracts was measured by BCA assay (Thermo Scientific) and the solution loaded to a polyacrylamide gel (Invitrogen). After electrophoresis, the proteins were blotted on a nitrocellulose membrane using the iBlot system (Invitrogen). The antibodies used to detect the proteins of interest were anti-hTau HT7 (Pierce MN1000), anti-pTauS422 (Roche) anti-pTauS404 (Abcam ab92676); these were detected by an appropriate HRP-conjugated secondary antibody. The complex was detected by luminescence using SuperSignal West Dura Extended Duration Substrate (Thermo Fisher) using an imager (Biorad).

REFERENCES

[0308] Ono, T. et al. A single-cell and feeder-free culture system for monkey embryonic stem cells. PLoS One 9, e88346, doi: 10.1371/journal.pone.0088346 (2014).

[0309] Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676, doi: 10.1016/j.cell.2006.07.024 (2006).

Example 1--Oligonucleotide Activity in Mouse Primary Neuronal Cell Cultures

[0310] Oligonucleotides targeting the part of SNHG14 long non-coding RNA which is antisense to the UBE3A pre-mRNA (position 55319 to 141053 of SEQ ID NO: 1) were tested for their ability to reduce the SNHG14 long non-coding RNA transcript preventing UBE3A expression (also termed UBE3A suppressor or UBE3A-SUP in the data table) and their ability to induce UBE3A mRNA re-expression in mouse primary cortical neuron cell cultures, obtained as described in the "Materials and methods" section above. The oligonucleotide concentration was 5 microM. The oligonucleotides were screened according to the protocol for screening in mouse cortical neuron cell cultures described in the section "Materials and methods". The results are shown in table 4.

TABLE-US-00008 TABLE 4 Oligonucleotide activity in primary mouse neuronal cell cultures. CMP % of Mock % of Mock ID NO oligonucleotide UBE3A_SUP sd UBE3A sd 95_1 CTCAtacttgctttaAT 3.6 0.1 154.1 15.1 95_2 CTcatacttgctttaAT 15.9 2.6 119.8 12.4 96_1 ACatctcatacttGCTT 4.0 0.5 149.9 11.5 96_2 ACatctcatacttgcTT 9.3 3.9 139.9 36.4 96_3 ACatctcatacttgCTT 3.1 0.2 143.2 3.9 97_1 ACatctcatactTGCT 4.0 1.5 154.5 10.0 97_2 ACatctcatacttgCT 6.1 1.7 141.1 14.1 97_3 ACatctcatacttGCT 3.7 0.6 162.7 15.0 97_4 ACATctcatacttgCT 5.2 0.4 156.7 24.4 98_1 TAcatctcatactTGCT 5.0 0.9 159.0 15.6 98_2 TAcatctcatacttgCT 15.5 5.3 130.4 3.4 98_3 TACAtctcatacttgCT 4.7 0.4 140.3 38.2 101_1 TACatctcatactTGC 2.6 0.5 152.6 10.2 101_2 TAcatctcatacttGC 19.2 6.0 112.0 15.0 101_3 TAcatctcatactTGC 3.5 0.4 117.2 13.7 101_4 TACAtctcatacttGC 3.0 0.7 140.5 12.4 100_1 CTAcatctcatactTGC 5.4 0.8 160.4 4.1 100_2 CTacatctcatacttGC 9.6 3.7 159.2 14.5 100_3 CTacatctcatactTGC 3.0 0.1 133.2 5.9 99_2 CCtacatctcatacttGC 7.8 1.4 150.7 11.0 99_3 CCtacatctcatactTGC 3.2 0.6 134.7 12.5 99_4 CCtacatctcatacTTGC 2.7 0.2 145.2 4.7 102_1 CCTAcatctcatactTG 5.8 1.7 127.0 24.5 102_2 CCtacatctcatactTG 20.2 6.6 129.7 9.2 102_4 CCTacatctcatacTTG 4.0 0.6 140.2 7.2 102_3 CCTacatctcatactTG 3.9 1.0 133.3 10.0 104_1 CCTacatctcataCTT 6.6 1.5 136.5 8.7 104_3 CCtacatctcatACTT 3.5 0.4 131.4 6.0 103_1 ACCtacatctcataCTT 5.8 1.4 130.8 0.7 103_2 ACctacatctcatacTT 11.4 2.2 123.6 12.4 103_3 ACctacatctcatACTT 5.8 0.8 132.2 4.5 105_1 TACCtacatctcatacTT 5.2 0.8 152.3 7.2 106_1 TTAcctacatctcataCTT 13.3 3.0 140.1 17.5 106_2 TTacctacatctcatacTT 21.0 1.4 116.9 15.0 107_1 ACCTacatctcataCT 6.2 0.9 119.2 3.4 107_2 ACctacatctcataCT 14.3 7.4 142.9 13.7 108_1 TACCtacatctcataCT 5.6 1.0 127.0 10.7 108_2 TAcctacatctcataCT 21.4 12.5 117.1 8.5 109_1 TTacctacatctcaTACT 4.4 0.4 138.9 1.2 109_2 TTacctacatctcataCT 22.9 3.3 117.1 13.0 110_1 TTAcctacatctcaTAC 8.7 2.1 133.2 5.1 110_2 TTacctacatctcatAC 21.0 5.1 111.4 11.1 111_1 GTtacctacatctCATA 8.0 2.4 143.8 14.8 111_2 GTtacctacatctcaTA 19.0 2.3 115.4 4.1 112_1 GTTacctacatctCAT 6.6 1.4 145.5 16.8 112_2 GTtacctacatctcAT 15.8 4.5 120.3 8.1 126_1 TCACtttccagatatCA 8.0 1.9 133.8 5.4 126_3 TCactttccagatatCA 53.4 75.9 112.0 11.4 128_1 ACATgtccctttataTT 16.3 2.5 114.7 11.1 128_2 ACatgtccctttataTT 14.8 1.1 136.9 6.2 129_1 ACAtgtccctttaTAT 11.8 1.9 135.0 14.3 132_1 CTCAtccctccaagaAA 9.1 1.6 131.7 8.4 132_2 CTcatccctccaagaAA 11.2 3.9 159.3 17.7

Example 2--Oligonucleotide Activity in Human Neuronal Cell Cultures

[0311] Oligonucleotides targeting human SNHG14 in the region downstream of SNORD109B corresponding to position 25278410 to 25419462 on chromosome 15 (SEQ ID NO: 1) were tested in patient derived human neuronal cell cultures (see protocol in "Materials and methods" section). The oligonucleotides ability to reduce the SNHG14 transcript in the region downstream of SNORD109B (also termed UBE3A suppressor or UBE3A-SUP in the data table), without affecting expression of SNORD115 was analyzed. Furthermore, the ability to induce UBE3A mRNA re-expression was analyzed.

[0312] The oligonucleotides were screened according to the protocol for screening oligonucleotides in human neuronal cell cultures described in the section "Materials and methods" above.

[0313] The results are shown in table 5. The expression of UBE3A mRNA has been measured for all compounds, whereas the knock-down of the UBE3A suppressor and the maintenance of SNORD1115 levels have not been analyzed for all compounds.

TABLE-US-00009 TABLE 5 Oligonucleotide activity in patient derived human neuronal cell cultures. Start % of Mock % of Mock % of Mock SEQ ID CMP Oligo conc Oligo conc Oligo conc NO 1 ID NO Target 0.2 .mu.M sd 1.0 .mu.M sd 5.0 .mu.M sd 1678 10_1 UBE3A 107 14 88 10 151 8 1679 12_2 UBE3A 100 9 87 14 158 16 1687 20_1 UBE3A 87 7 102 22 213 44 1712 21_1 UBE3A 127 23 166 6 178 13 1712 21_1 UBE3A-SUP 81 3 82 8 72 12 1712 21_1 SNORD115 115 6 142 24 169 26 4167 22_1 UBE3A 87 5 90 8 146 20 4170 27_1 UBE3A 94 16 106 11 170 10 4171 29_2 UBE3A 86 13 100 12 194 35 4172 30_1 UBE3A 96 6 121 12 209 27 9210 35_1 UBE3A 88 5 112 23 195 27 10838 37_1 UBE3A 77 7 85 9 169 24 15565 38_2 UBE3A 93 11 108 6 167 34 22209 42_1 UBE3A 125 16 143 14 180 17 22209 42_1 UBE3A-SUP 108 14 98 15 85 18 22209 42_1 SNORD115 101 14 93 25 127 21 30449 43_1 UBE3A 99 5 95 13 115 8 30451 44_1 UBE3A 99 15 80 20 141 17 30451 44_2 UBE3A 98 31 104 16 119 7 30697 46_1 UBE3A 91 8 87 5 167 20 36066 49_1 UBE3A 95 6 111 10 155 29 36066 49_1 UBE3A-SUP 76 7 84 24 110 31 36066 49_1 SNORD115 99 14 111 20 94 6 36068 50_1 UBE3A 109 15 105 11 92 14 36068 50_1 UBE3A-SUP 122 24 93 28 73 7 36068 50_1 SNORD115 120 15 113 12 99 6 37206 51_1 UBE3A 114 16 101 7 101 3 37206 51_1 UBE3A-SUP 128 21 67 9 84 13 37206 51_1 SNORD115 140 26 110 9 100 11 46130 52_1 UBE3A 139 3 160 1 236 36 46130 52_1 UBE3A-SUP 135 16 133 26 160 32 46130 52_1 SNORD115 104 8 119 14 100 8 48145 59_1 UBE3A 179 3 122 17 115 NA 48170 76_1 UBE3A 85 16 100 8 155 12 48171 80_1 UBE3A 120 7 114 10 172 20 48171 78_1 UBE3A 136 31 103 20 169 11 48172 82_2 UBE3A 96 11 121 4 186 32 48172 84_1 UBE3A 95 14 100 8 158 14 49343 85_1 UBE3A 97 22 121 10 189 17 49722 87_1 UBE3A 111 9 126 11 177 22 52417 92_1 UBE3A 133 7 140 30 140 8 52417 92_1 UBE3A-SUP 88 14 80 14 82 8 52417 92_1 SNORD115 102 8 114 20 91 9 52420 93_1 UBE3A 111 14 120 9 126 16 52420 93_1 UBE3A-SUP 104 23 82 20 79 8 52420 93_1 SNORD115 110 11 114 17 95 7 53953 94_1 UBE3A 117 12 147 15 166 15 53953 94_1 UBE3A-SUP 92 18 81 5 86 22 53953 94_1 SNORD115 124 33 122 17 106 14 60819 95_1 UBE3A 103 11 131 14 175 7 60819 95_1 UBE3A-SUP 93 13 87 3 74 6 60819 95_1 SNORD115 162 19 158 20 201 11 60819 95_2 UBE3A 147 10 129 20 117 2 60819 95_2 UBE3A-SUP 118 24 87 13 83 8 60819 95_2 SNORD115 104 17 118 10 129 6 60823 96_1 UBE3A 115 16 135 19 174 17 60823 96_1 UBE3A-SUP 104 25 93 32 91 11 60823 96_2 UBE3A 108 7 114 9 115 13 60823 96_2 UBE3A-SUP 99 17 92 19 93 10 60824 97_1 UBE3A 111 12 134 23 169 14 60824 97_1 UBE3A-SUP 110 27 105 33 92 10 60824 97_2 UBE3A 124 13 126 12 124 11 60824 97_2 UBE3A-SUP 113 17 107 33 96 20 60824 98_1 UBE3A 111 16 119 11 138 14 60824 98_1 UBE3A-SUP 118 34 98 23 82 19 60824 98_1 SNORD115 109 11 123 18 114 16 60824 98_2 UBE3A 128 10 109 7 136 12 60824 98_2 UBE3A-SUP 91 15 77 11 110 16 60824 98_2 SNORD115 101 3 110 7 124 11 60825 99_1 UBE3A 125 6 115 5 131 10 60825 99_1 UBE3A-SUP 139 18 121 34 127 45 60825 99_1 SNORD115 110 18 112 12 99 19 60825 99_2 UBE3A 120 21 111 11 135 22 60825 99_2 UBE3A-SUP 96 21 79 15 75 11 60825 99_2 SNORD115 104 34 113 22 131 24 60825 100_1 UBE3A 123 34 139 34 145 21 60825 100_1 UBE3A-SUP 104 37 127 46 99 17 60825 100_2 UBE3A 124 46 138 37 145 31 60825 100_2 UBE3A-SUP 111 36 120 47 92 11 60825 101_1 UBE3A 112 18 123 15 150 13 60825 101_1 UBE3A-SUP 96 18 102 14 88 12 60825 101_2 UBE3A 118 15 138 24 139 32 60825 101_2 UBE3A-SUP 100 29 110 39 92 10 60826 102_1 UBE3A 132 17 120 7 125 9 60826 102_1 UBE3A-SUP 113 16 83 5 88 18 60826 102_1 SNORD115 121 36 131 23 100 9 60826 102_2 UBE3A 90 6 116 23 103 7 60826 102_2 UBE3A-SUP 91 7 90 12 64 18 60826 102_2 SNORD115 116 15 146 27 183 28 60827 103_1 UBE3A 106 8 112 10 115 9 60827 103_1 UBE3A-SUP 99 15 110 28 94 8 60827 103_2 UBE3A 107 14 120 13 112 14 60827 103_2 UBE3A-SUP 97 14 118 38 93 20 60827 104_1 UBE3A 128 14 111 9 111 6 60827 104_1 UBE3A-SUP 111 12 97 9 87 19 60827 104_1 SNORD115 114 10 110 12 109 13 60827 104_2 UBE3A 108 10 111 16 109 10 60827 104_2 UBE3A-SUP 103 13 103 33 89 9 60827 105_1 UBE3A 122 13 121 12 121 4 60827 105_1 UBE3A-SUP 119 7 97 15 93 7 60827 105_1 SNORD115 114 21 128 12 118 9 60827 105_2 UBE3A 123 5 110 9 114 8 60827 105_2 UBE3A-SUP 110 11 89 17 94 21 60827 105_2 SNORD115 102 15 108 16 107 18 60827 106_1 UBE3A 114 17 133 23 125 9 60827 106_1 UBE3A-SUP 112 35 103 15 87 12 60827 106_2 UBE3A 110 12 130 22 123 14 60827 106_2 UBE3A-SUP 105 19 107 27 93 10 60828 107_1 UBE3A 83 11 117 13 112 6 60828 107_1 UBE3A-SUP 86 11 114 16 67 7 60828 107_1 SNORD115 108 17 130 21 137 24 60828 107_2 UBE3A 143 42 117 10 122 11 60828 107_2 UBE3A-SUP 116 12 92 4 100 8 60828 107_2 SNORD115 108 4 127 16 108 14 60828 108_1 UBE3A 120 7 127 31 132 31 60828 108_1 UBE3A-SUP 153 33 118 34 89 17 60828 108_1 SNORD115 114 9 114 9 105 15 60828 108_2 UBE3A 122 18 133 26 128 9 60828 108_2 UBE3A-SUP 101 19 100 28 89 17 60828 109_1 UBE3A 108 10 129 14 128 5 60828 109_1 UBE3A-SUP 106 21 107 24 84 8 60828 109_2 UBE3A 109 11 110 8 111 13 60828 109_2 UBE3A-SUP 95 15 86 14 83 9 60829 110_1 UBE3A 104 6 83 3 101 15 60829 110_1 UBE3A-SUP 100 13 95 12 79 4 60829 110_1 SNORD115 126 21 125 6 182 13 60829 110_2 UBE3A 92 7 87 8 96 7 60829 110_2 UBE3A-SUP 99 7 108 9 81 5 60829 110_2 SNORD115 118 15 139 22 198 39 60830 111_1 UBE3A 110 6 122 13 124 10 60830 111_1 UBE3A-SUP 104 14 90 28 79 11 60830 111_2 UBE3A 115 10 120 15 121 10 60830 111_2 UBE3A-SUP 114 20 89 19 87 9 60831 112_1 UBE3A 93 8 94 13 106 10 60831 112_1 UBE3A-SUP 97 1 68 29 82 7 60831 112_1 SNORD115 116 20 110 13 158 20 60831 112_2 UBE3A 83 8 78 7 83 6 60831 112_2 UBE3A-SUP 106 35 80 23 69 9 60831 112_2 SNORD115 107 6 106 8 159 21 62198 113_1 UBE3A 110 3 122 6 134 9 62198 113_1 UBE3A-SUP 113 20 85 19 79 24 62198 113_1 SNORD115 116 18 123 9 91 9 62284 115_1 UBE3A 105 14 98 19 141 36 62422 116_1 UBE3A 130 19 142 29 172 18 62423 117_1 UBE3A 76 8 93 13 171 17 62439 118_1 UBE3A 75 7 88 9 150 19 66378 119_1 UBE3A 96 14 93 5 110 10 77565 126_1 UBE3A 94 6 113 5 125 14 77565 126_1 UBE3A-SUP 83 17 95 33 85 5 77565 126_1 SNORD115 105 11 123 19 152 15 77565 126_2 UBE3A 95 5 126 9 111 2 77565 126_2 UBE3A-SUP 77 27 106 21 83 15 77565 126_2 SNORD115 115 17 157 13 180 15 92321 128_1 UBE3A 102 7 91 5 111 13 92321 128_1 UBE3A-SUP 115 3 104 25 91 13 92321 128_1 SNORD115 135 9 132 12 196 35 92321 128_2 UBE3A 91 5 96 8 104 8 92321 128_2 UBE3A-SUP 112 20 92 20 79 7 92321 128_2 SNORD115 125 7 111 13 169 12 92322 129_1 UBE3A 101 5 103 2 110 7 92322 129_1 UBE3A-SUP 99 39 113 12 94 13 92322 129_1 SNORD115 124 25 114 6 140 13 92322 129_2 UBE3A 93 2 100 4 113 16 92322 129_2 UBE3A-SUP 109 4 102 22 85 7 92322 129_2 SNORD115 103 11 99 9 152 31 97154 132_1 UBE3A 100 10 128 13 142 13 97154 132_1 UBE3A-SUP 103 9 115 8 109 6 97154 132_1 SNORD115 49 7 90 12 143 25 97154 132_2 UBE3A 111 8 128 17 128 17 97154 132_2 UBE3A-SUP 95 7 116 9 105 13 97154 133_2 SNORD115 86 7 106 9 121 9 97154 133_1 UBE3A 101 3 107 11 124 19 97154 133_1 UBE3A-SUP 112 9 117 7 146 25 97154 133_1 SNORD115 60 7 110 15 141 15 97154 133_2 UBE3A 94 13 116 14 138 12 97154 133_2 UBE3A-SUP 116 6 128 13 148 38 97154 132_2 SNORD115 70 5 108 9 160 34 106137 137_1 UBE3A 83 12 74 11 124 20 109404 138_1 UBE3A 80 20 92 7 120 21 110766 139_1 UBE3A 76 5 85 12 121 17 114826 140_1 UBE3A 87 10 88 11 136 9 118637 143_1 UBE3A 83 7 104 30 141 28 118639 144_1 UBE3A 74 17 31 39 106 33 124160 145_2 UBE3A 89 6 95 10 115 25 125499 146_1 UBE3A 83 13 76 7 124 16 125499 146_2 UBE3A 123 30 79 14 102 23 125538 150_2 UBE3A 82 17 82 7 119 24

[0314] Of the 187 compounds tested approximately 90% showed re-expression of UBE3A when compared to the mock oligonucleotide at the 5 micro Molar concentration. The number of oligonucleotides capable of inducing re-expression of UBE3A is higher in the region between position 1 to 55318 of SEQ ID NO: 1 (non-overlapping region) then in the region complementary to UBE3A coding region (overlapping region. FIG. 2 plots the distribution of the oligonucleotides according to their position on chromosome 15 versus the UBE3A mRNA expression relative to the mock oligonucleotide.

[0315] For the oligonucleotides where SNORD115 has been tested there is no significant down regulation when compared to mock at 1 and 5 microM.

Example 3--Generation and Validation of Cyno Neuronal Differentiation In Vitro System

[0316] Cyno IPSCs were induced to differentiate into NPCs following the protocol as described in the Materials and Methods section and depicted in FIG. 4. The protocol allows to derive NPCs which can be maintained and expanded in basal medium supplemented with FGF, EGF and BDNF. To verify the efficient induction of cyno NPCs, expression of the neural stem cell markers SOX2 and NESTIN was evaluated by immunostaining (see FIG. 5A and FIG. 5B). Cyno NPCs express SOX2 and NESTIN and the expression pattern is highly comparable to human NPCs. To derive differentiated NCs, expanding NPCs are dissociated and plated in SFA medium for a week. Afterwards the cells are exposed to differentiation medium (BGAA). To evaluate the neuronal differentiation potential of cyno NPCs, a transcriptional analysis was performed and results were compared to the transcriptional profile of differentiated human NPCs (see FIG. 6A, FIG. 6B, FIG. 6C, FIG. 6D). After 14 days of differentiation in BGAA medium, cyno cultures show decrease in the expression of pluripotency markers like NANOG and POU5F1 and increased expression of ectodermal and neuronal markers like PAX6, SOX1, ZIC1, ASCL1, TUBB3 if compared to cIPSCs (see FIG. 6A). Similarly, transcriptional analysis (RNA sequencing in FIG. 6B and qPCR in FIG. 6C) of human pluripotent stem cell-derived NCs differentiated for 14 days in BGAA reveals decreased expression of pluripotency markers and increased expression of ectodermal and neuronal markers in day 14 BGAA differentiated NCs if compared to hESCs. Importantly, changes in the expression of UBE3A, a disease gene implicated in Angelman syndrome, and UBE3A-ATS, a non-coding transcript described to be expressed in neurons, are comparable between differentiated cyno and human NCs (see FIG. 6D). These data demonstrate that cyno IPCS differentiate into NCs and the expression profile of specific markers is similar to differentiated human NCs.

Example 4--Dissociation and Reseeding of Differentiated Cynomolgus NCs

[0317] Stem cell-derived cells have a great potential as in vitro system for drug screening, especially if the corresponding primary cell type is not available or only in limited quantities as it is the case for neurons. However, drug screening requires robust cellular screening systems, which are available in suitable plate formats. For this purpose, we tested different strategies to differentiate NPCs derived from NHP-IPSCs into neuronal cells (NCs). In order to identify a robust cellular system with uniformly distributed NCs, expanding NPCs were dissociated and exposed to differentiation medium in one of two ways: either they were plated directly in the final assay format or, alternatively, the cells were plated in a cell culture flask and, thereafter, dissociated and reseeded into the final assay format after further differentiation (see FIG. 7). In method A and B, NPCs were differentiated for 23 days in flasks and then dissociated and reseeded in the assay format. After an additional differentiation period of 7 days cells were treated with oligonucleotides. Alternatively, in methods C and D, NPCs were dissociated and directly plated onto 96 well plates. The differentiation medium in method A and C was supplemented with a mitotic inhibitors cocktail (Uridine 35 .mu.g/ml and 15 .mu.g/ml 5-Fuoro-2'-deoxyuridine) to inhibit cell proliferation. On day 35 cultures were fixed and stained for the neural progenitor cell and glia cell marker SOX2 and neuronal marker MAP2 (see FIG. 8). The method B (with replating and without mitotic inhibitor) resulted in a more uniformly distributed cell culture with MAP2 positive cells and with fewer SOX2 positive cells than in the other methods. For this reason, the neuronal differentiation method B was chosen for subsequent described oligonucleotide treatments.

Example 5--Dissociation and Reseeding of Differentiated Human NCs

[0318] As described in Example 4, we established that replating differentiating cyno neurons was a necessary step to make these cells amenable for screening purposes. We therefore set out to verify whether it was possible to utilize a similar procedure for human IPSC-derived neurons. To this end, previously established neural progenitor cells (NPCs) from two IPSC lines (ED4 and SFC808) were differentiated following the procedure described in the Materials and Methods section. Briefly, expanding NPCs were dissociated and plated in SFA medium for a week. Afterwards the cells were exposed to differentiation medium (BGAA) in one of two ways: either they were plated directly in assay format or in a cell culture flask. In the latter case, the cells were differentiated for 21 days and then dissociated and reseeded in the assay format. Both experimental groups (i.e. directly differentiated or dissociated and reseeded) were differentiated for a total of 6 weeks (6 weeks in assay format or 3 weeks in flask and 3 weeks in assay format, respectively) before being processed.

[0319] For a first set of experiments, the neurons were analyzed by immunofluorescence and high content imaging. The markers used were specific for neural progenitors (SOX2), glial cells (GFAP) or neurons (MAP2 and HuC/D). As exemplified in FIG. 9, the replating process did not seem to influence the differentiation or morphology of the human IPSC-derived neurons. This was further verified by the quantitation of the staining, which showed no significant difference in the presence of HuC/D positive cells in the direct differentiation and replated groups (FIG. 10; for ED4, p=0.542, for SFC808, p=0.579).

[0320] To further confirm that it is possible to dissociate and replate differentiated human IPSC-derived neurons without altering their features, we wanted to confirm that cytoskeleton-associated proteins were not disrupted by the dissociation process. We analyzed the expression of the microtubule associated protein Tau as it is crucial for neuronal function and is directly implicated in neurodegenerative diseases such as Frontotemporal dementia and Alzheimer's disease. The cells were cultured as described above (with and without dissociation and reseeding), and the protein extracts were analyzed by Western Blot. As shown in FIG. 11, the constant expression of Tau and two of its phosphorylated forms proves that replating did, surprisingly, not disrupt the cytoskeletal characteristics of human IPSC-derived neurons, suggesting that physiological features will not be altered by the process.

[0321] These results demonstrate that human IPSC-derived neurons can undergo dissociation and reseeding during differentiation and maintain their key features, suggesting that the replating step (dissociation and reseeding) is feasible for the differentiation of primate stem cell derived neurons.

Example 6--High-Throughput Screening Using Primate IPSC-Derived Dissociated and Reseeded Differentiated NCs

[0322] The primate IPSC-derived neuronal cultures described above are highly flexible and reproducible systems amenable to be used for screening purposes. We developed a workflow to treat the cells with oligonucleotides and verify the expression of target and associated genes by quantitative polymerase chain reaction (qPCR) (see FIG. 12). To this end, either human or cynomolgus neural progenitors were treated as described above. For screening, the cells were dissociated after 3 weeks of differentiation and reseeded in 96 well plates at the density of 200'000 cells/cm.sup.2. After one additional week of differentiation, the cells were treated with oligonucleotides for 5 days and then processed for expression analysis as described in the Materials and Methods section. This method allows constant availability of samples to be tested in a high-throughput manner.

[0323] For this example, we utilized 5 different oligonucleotides that target the Ube3A antisense (Ube3A_ATS) transcript at 8 concentrations, starting at 20 .mu.M and decreasing by 3 fold at each step, resulting in doses of 20.000, 6.325, 2.000, 0.632, 0.200, 0.063, 0.020 and 0.006 .mu.M. We tested these compounds on neurons differentiated from Angelman Syndrom patient IPSCs (human) or cynomologus IPSCs (cyno).

[0324] After the treatment, the cells were lysed, the RNA was extracted and the qPCR reaction was performed by using commercially available TaqMan assays for Ube3A and Ube3A_ATS; GAPDH was used in human cells and TBP in cyno cells as housekeeping gene to normalize the expression data. In all cases, the treatment resulted in a marked decrease of Ube3A_ATS, which was matched by increased expression of the sense transcript. This effect was evident in a dose-response manner; for simplicity of visualization, we report in FIG. 13A, FIG. 13B, FIG. 13C,

[0325] FIG. 13D the expression levels at a high (2.mu.M) and a low (0.02 .mu.M) dose. At the dose 2 .mu.M, the antisense transcript was expressed at .about.50% of the level of vehicle (PBS) treated cells; this was true for both human and cyno cells. The knockdown of Ube3A_ATS resulted in the increased expression of Ube3A, to levels of .about.250% and .about.130% of vehicle for human and cyno, respectively. This discrepancy is not surprising, as the patient derived neurons, because of the deletion of one allele of the Ube3A gene, express very low levels of the sense transcript.

[0326] These experiments demonstrate target engagement of oligonucleotides directed at Ube3A ATS in human and cyno neurons. Therefore, the method described is suitable for high-throughput screening of therapeutic oligonucleotides in primate IPSC-derived neurons.

Sequence CWU 1

1

1501141053DNAhomo sapiens 1agatgtgcgg ccctccaata ggacaaataa gactctcaga gcctggctct atttggggat 60ccctcagtga caacatagta cccctgtgag cgtgcctttt ctatctcttc gaagagggca 120gtggcatcct gtcttatgag tcagtgtgca ctttagtgtg cctagtgacc caagacttgc 180tttaattgta gatagatact tacatatagg aaatatttct taagtaacaa atgaaaaact 240ttagaagatt gaattaaggg tcaagcaact gtgatatgtc tgaaaatctc attagtgttg 300tgctgaaaga aggaaatatg gcatgcctct attaaataat gacagtggaa ccaagtttat 360tgctttgtta tttttactgt ggagtatttt ctaagattat ttttgctttt tttttctttc 420atgttttgct gagatagaag gcctggaatc tgatcctcca cttcagagaa caggggtgag 480tagctaagcc attatctttt gaaattcata tgtcatgtgc tctttgctag gtctttaggt 540cgttttgtac atcttttcag aagcttattg gaggacattt tcatgatatg tccttttcct 600cattgagacc ctcaccatgt cacctacact attgaatcct tatcatttct cttttaattt 660taactctctt ttgcttttat ggaaaaatgt agaatttaag agaatttttg gcaatttcat 720attggatcaa aatgtattgt agtgaaatcc aggtgtgcca aaatattaac agattttccc 780catctgttta attattgggg tttcagaata gagactccat ggttcataat atctttgtgg 840tcatactaca ttatatttct gcttctaatt taattattaa atattgactt gaattagtct 900tttcctcatt gttgcaacaa ggtaagttat ataggaaatt ttcttctctt gatggcatgt 960ctgagataat catagatata agacacctgg ctggtttcta gaatgcatgt aatttttatt 1020tcttgatctg tgtgttgagt acttgctgtg attgcatcat gcaaatacat tgagctttac 1080aattttagtg tatgcacttt tctacatgta tattattctt cgatagaaag taaaaaaaac 1140ttatcgaact agtcaaaata ttggttaata cataaaaaaa gtctcaagta gattgtgtat 1200tacatggtgc ttgttgattg atgccctcat aatagatcaa gtgggttctc tctttagcac 1260agggcttttt agcaaatcat gtcatgagta gttactcaag tatttttatt ttaacacatt 1320tatatttttt ctatgtatat tcttaaattc tcttatactt ttttctctgt tataaaaaca 1380tgctgaacaa tctcaagtct taaggattgc agtattgtcc ccacatattc atgtattttg 1440gtactcaatt ctttatactt tctttgacag atcacttgaa ctggcacatg tctcttgttt 1500tgcagagagg gaattaatgt gataccttca tgcttttcta ttctatgtgc tacataattg 1560aatatacaag caaatatagt tgttaagatt tagtgtgatt atttctacac cacatgcaaa 1620gaagtttctc atagatctta atagaggccc acatgcattg tacagtttag aatttgggga 1680aatattgatg aagttgggta aagtataaag ccaaaagtca gaacagtgaa ctccttgctt 1740aaggatttcc ttggagatta cttagtcaat acacaactga taaatttaag tgcttttcac 1800cttttgagtt ctcgacatac taaagctaaa atgtgtttca acttttaatc ctgcttccct 1860gattttccct tttttagtct gagatcaaag agtttcagcc ataaattact gccaagagta 1920atcacttcat tttaagaaag cttaacaata tagaagaata taaaattatt tatgacagat 1980gtatttttaa ccttttcccc atgctttcca gaggaaatat gtttaatcat ctgccctata 2040ttagggaaaa actttctatg ctaatacaag tatctatcaa tccatttatc tttctatata 2100agatgtattg atcataacca attaacttta ctgtaaatga gctttagatt tgacattttg 2160gtagtaatat atgttgtaca atctcctgag gtcctatagg tcttgaggtc tctatgtcaa 2220aaactataga tgtgccagtg tcctcagtga atgtgaagga caccacattt tccttagcca 2280tttcttgttt tcagaataat ggttatcaac attttgctac tgcaagatac catacattta 2340taatcggaat atgccagttt ttatgcactc atgcctctgt ttctgtagag cattcccaga 2400atgagtaatg cttgaaaatt aggtccatgt gatttcttta atgagttata gtcaaatcat 2460gaaatattca ggttaccatt atttcagtaa tgtattagaa tgtcaaggta aagttatcta 2520cattgtatat atacacacaa catagatata atttatacaa catctatatt tatacaacag 2580atatatattt atgtatatat ttatacaaca taaaatatat tttattattt aaacataaaa 2640tatattttat tatttaatat agattcttaa gtgataaata tgtttaatat tattaaaata 2700ggttaaaata ggttatagtt agtacagtga aaattggcag cccttttaca aaacatatgc 2760cataactata gcatttatca ctgacagtca taccaggata gtcttttatt tccaatcact 2820taaatattcc taattgcaaa agaaatttga agactaaaat tcagaagttt tgaaagagcc 2880attgcctggg taaactatac aggtttcagt tttatttata ataattatga ggccaggcgc 2940agtggctcac acctgtaatc ccaacacttt gggaggccga ggcgggtgga tcacgaggtc 3000aggagatcga gaccatcctg gctaacacgg tgaaacccca tctctactaa aaatacaaaa 3060aattagccgg gcgtggtggc gggcgcctgt agtcccagct actcgggagg ctgaggcagg 3120agaatggcgt gaacctggta ggcggagctt gcagtgagcc gagatcgcgc cactgccctc 3180cagcctggga gacagtgcga gactccgtct caaaaaaaaa aattatgtat atatttataa 3240attaatactt aataaattaa taacttgtga taggcaatgc aaagatgaca gtaaaaggac 3300aaaattgatt agattgataa agtcctgtta acatgaagaa attgaccagg ataccatcca 3360cactataaag ttagagaaat ttatcaggac aattccctaa aatactcttc tcaattttaa 3420cattgtaaca ggaattttta aaattttggt attatgtgtg tttccttcca gataatttga 3480acagattcat atttggtatt tttaaaagcc atatctttgt ccttagtgct ggcaatgtat 3540tcttgagaat gaacaaataa gagatacgta aaagcataag agaaggtatc aggttgaagt 3600agtcaatcag ttatacagaa cacaaagaat tttatcttgt ataatgttta tatagcttta 3660tagaagtgtg ctgaaagggc tataaaacat ggacattatt atctcattga aaggtccaat 3720acgtactgaa atacatgctt ttattttgaa ccaaccaccc tataaacgtt gtatggctta 3780tttagatgag agcccaggtt gtgtgtgtct gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 3840gtgtgtgtgt gtgtacctga cagggaagca agaacatcga gttgccaatg cactctgtct 3900atggttagaa tcatgctgaa aacatggctc ccccagttct ggaatgagcc cacagatcaa 3960gcattcccca aagacatagc aggctcaaat ccctgtgtac acaatatttt atgattatct 4020tatgtcagta ctttcaaagt atacagtttg tgtgaagaca aatccaatgt catttttctt 4080ggctagccta tatgtgtggt aaatccatta tttacttact tgcttcctga aaattacaat 4140tagattaaca aactgcagca aagtgggcat gatgagatag agattgaagt gtaagcttat 4200gttaatgatg cccttggttt ggataaacac atctaagaga aaaatggaaa aacacacatg 4260gcagggaagc cttgatagag ccaaaatata ggattgtatg tagtaatgca atccatagat 4320gagcatttgg cagtaatatt atttttcaga tatggataaa aattgcttag gagagtaaag 4380agagacaaag ttgaaagcag gtttatagta ggtgttgttt tagtgttgat ccctttttgc 4440tccaataatc aaagtgataa atattgaaaa ttgattcatg cagcattact tactccattc 4500taatttttat atatgtcaaa agtgccatct cccaaactgt gctatcccct tcaggagaag 4560agactctgct gaagtttata aggttgacat attgccagct tcaataatgt aaagatgaag 4620tgtatactga attcttaatg caaataacaa ctctattgga aagtaaccca gttatagaag 4680tgctaatttg tcaggagctg ccttaccaag atcatgatga gtacagttat ctcaggattc 4740tgaaagattg ttttccgatt tcaactagtc tagctgaatg ttccttgata gaaagagagg 4800acttttagaa ttggttcaat atgatgacct cctgaattat ctcacatagc ccgtttgtac 4860atgcctttct tttctctcag aaaatggcac tatcataata gctttcttac acagacttca 4920ccttagggtt ttacattaag ggaggggtct ggtgtttcat ttattttgaa gtatttgttg 4980ttgattgtgt acagtgcttg agtaaaaaat tgaatataga aacatctaga atattttttt 5040aaaggatcag tgtttataaa gtgaattatt agtgtcaata atgttgggaa agttttaaga 5100gaatatagga aacttgaaca ttacacaact acaatgggac caaattgtgg ggtctcatta 5160tagttaatat ttatgtattt ttttccaatt gatttgtgtg ctttttttct gcatgttttt 5220ggcagataga atggctataa caagtaacag catgtcaggt aataaaaata agcagagccc 5280tattccttta aaaatcttca ctgatgggag ggccataaaa taagtcttaa tacatttaaa 5340gaattaaatt catgtaaacc atgttaattt aattccacaa tgatattgaa ttagaaataa 5400gaggaatatc tcttgaacat ctcctaaatg tttggaaatt taaattagca tttctgacct 5460atttattggt taaaaaagat acaaagaaag gaaaattgaa aagtcttttg aactgaataa 5520aaataaaaat atagaatcta aaactttatg ggatactgac aaaacaggat atagggaata 5580atttatagca ctgaaatgcc tatattagaa aagaaaaaag gttttaaatc agtaaatttg 5640tattttacct taagaaactt agaaaagaac aaattaaccc agacttaagt aaaataaagg 5700cactaataaa gataagagca gaaatcaatg aaatataaaa caacaaaaca cagagaaaaa 5760ttgagaaaat ttaaaaatag cctagtgaga agatattgat aaacttgtaa ccagaccaat 5820ttaagaaaaa aagtcaaaac acaaatacca atatttgaaa atgtaggagg gcaaatcatt 5880acagattcta tgaatactaa aatgataata aggaaaaatt atttaaaagg ggcatgtcag 5940ccaggcatgg tggcttaccc ctgtaatccc agcactttgg caggccgagg tgggaggatt 6000gctagagctc aggcattcga gaccagcctg ggcaacatgt tgaaaccttg tctacacaaa 6060aagtacaaaa attagctggg tgtggtgttg cacacttgta gtcccagtca cttgggaggc 6120tgaggcgaga ggatcacttg agcccaggag gttgaggctg cagtgagcca tgtttgtacc 6180actgccctcc agcctgggtg acaaagtaag accctatgta aaaaaaaaaa aatgtatgcc 6240aacatttttc aataacttaa atgaaatgga aaaattcctt gaaagacacg aactacaaaa 6300actcagtgaa caagtaaata acctgaatag ccctgtatca agtaaattga atttgtagtt 6360aaaagccttc caacagagaa aacttcaggt acctatagct tcatatgaaa tgaaaaaaaa 6420aataccaatc ctctacaaga ttccagaaca tttaaaagaa gggaatattt cccaacttat 6480tccatttgga cagcaatacc caggtaagaa aaagagacac agaaatttaa aaagaagaat 6540atacattatt ccttaggaac ataaatgcaa agaaatctaa tcaaaatttt ggcaaatgaa 6600atgtagaaat actttatgac caagtgagag ttacccaaag aatttaaggt tggttttata 6660tgtaaagatc aaccaatata ggaaaatcac ttctggaaag tcagagtaag aaactccaaa 6720aatctactcc tccataaaac caataacagc cttgatagaa atagttgaaa ttaattttcc 6780aaaactttgg aaattaacca aaggcttaca aaattccaga gaacattaat tcaagaaaaa 6840tggctgaatc agtaagaaca gccagctttg tggcatttta atatgacccc ttcccatgct 6900tttctcccta gtgctgagat agtcttaaaa attagcagga tagcaaccac tggagaagaa 6960aggtttggaa atttcccaaa aagttccatc cccatagaat tatcactatt tgacctctaa 7020agcccaatct ataggattta tattcatttg gactgactca gagctcactc agtaaggaaa 7080tctcaatttc aaggtattgg tcaaaaagaa tccatggcaa ttgttgacta tcacaactgc 7140ctgaagtctt ggtaacagtt gggataaaca agaagctgat caaaaactga aaactaaaat 7200cttgggaatg agatatctac aggatgcttc aaaaagcttt gatacattcc tgtttatcta 7260gaaagctaca tgcaggctgg gtgcagtggc tcacacctgt aatcccagca ctttgggagg 7320ccgaggcgtg cggatcatga ggtcaggagt ttgaggccag cctgaccaac atggtctcta 7380ctaaaaacac aaaaattagc caggcgtggt ggcgtgcatc tgtaatccta gctactcagg 7440aggctgagac aggagaatcg cttgaacccg ggaggcggag gttgcagtga gccaagattg 7500tgccactgca ctccagcctg ggcgacagag caaggctctg tctcaaaaaa aaaaaaaaaa 7560aaagccacat gcatgtaatt gtttacctct ggctttcctt ttatgctctg ggcaagctaa 7620ggaagagttg tgaactacct aagtgctgaa tgggaaccat aacacacaca cacacacaca 7680cacacacaca cacagcacct tagtaaaggg tgagaggcat gttagttaga agcattaaag 7740gaaatctctt tctagtcatt atctgtgcac taacctaact gagcagagac ttcagtatcc 7800acatactaca gggcatataa actttacaga attagtccag gaaaatcata tctaaaaaaa 7860aaaaaaagca gtaacaaaaa taaactctgg gaaaggggag aatatgattt aaagagttgc 7920cacattatac ataatatgtc tagtgttcaa caaaaaatta cgagacatgc aaagaaatag 7980aaaaatatgg cacaaagagg ataagatgaa gtcagtgaaa ctatcctcga ggaagaccag 8040atgttggtct tactagacac agacattgaa ccagctatta aaaatacgta cacagaacta 8100agaaaaacat gtcaaaaggg ttaaagaaag gtataaaaat agtgtcttac caaatataga 8160ctaccaataa agagatagaa attataagaa aagacaacat gaaaaaatat aaagcaaaaa 8220aattagacaa ttgaaacaag agggcctcta ttcgcagatt tgagcaggca gaagaaagaa 8280tcagtgaact tgaagatatg tcaactgaga ttatccagtc tgagcaacaa aggtgggaaa 8340aaatgaagaa aactgagcaa caaagaactg tagaacagca tctctcatac caatggatac 8400ataaactgga gccctagaag gatgaaaaaa ggagaaggaa agaaaacttc ccaaatttta 8460agaaaaacat taatttatat acccgagatg accaataaaa tccaattaag ataatctcaa 8520agagaccaac acctatacac atcatagtca gcgtgtcaaa agacaaacat aaggagagaa 8580ttcttgaaga tagtaagaaa aaaattattc ataacataca caccatcctc aataagtctg 8640acaattgact tctcactgta aaccatgcag gctaaaaagg caatgtacat aaccaaagtg 8700atgaaataaa aaccttcaac caatcattct agatccaaca aaactattgt tcaaaaagaa 8760gaaatgaaga cattcctaaa caaaatctca gagaaatgtt ctctataaga cttgtcctaa 8820tagaaatgct aaaggaactc cttcggtctg aaatagaaag gcactggaga gtaaatcaaa 8880tccacgagaa gaaataaaga gaaccagtat aagtaactac atgtgtaagt ttaaaacaaa 8940gtataaattt attttgtttg taacatttgt cttttcctat ttgatttaaa atataatctc 9000aattataaac ttgtgttgat ggtattatat aaagatgtaa ttttgggtta tagcaccaaa 9060atggcagaat aggaattttc tgtaggtgtt tcccacataa gtatcaattt tgacaaccat 9120ccatgggcaa gagtaccttg tgggagttca ggagttgaca gtaaaacttc agcacaccag 9180aggagtaaag aaatctaaga atagattcat tggaaagggt ataaacagtt tcactttacc 9240tgcatcacca acccccaaaa gtggcacagc tcagtaacaa gagcccatta tttcttccac 9300agaggaaaag gagagtataa taagtaagtg tccagtttct caagacatac aggcccctgc 9360ccaagagatc cactttattt tcatctcacc cagaatattg aggtgatcag caaggtggag 9420tggttgggag agggtaaaag caggaaagag agatggggac tcaaacagca gcccatactt 9480ggaactgcca tagatcctac cagttacttc atggactcca tcaggaaccc acctataagc 9540cacaggggat gcatccctcc catcttgcca acaaaacccc gaatgctcca aatgcctcac 9600ccactctttg gctggctccc aagtgcgctc ctgtgaaaag cgagtgagta tctctgcaga 9660tggcttgcaa gcacatgttg acagctggct ccactctgta gaactggaaa aaagctcaca 9720tacatgagaa tttcaggaca ctaccctaaa aaaaaaaatg agacttcagc acctggcctg 9780gctttatgca acctagagaa ggtgatatga tttggctctg tgtccccacg taaatctcat 9840gtcaaattgt aatccccacg tgttggacaa gggacttggt gggaggtgat tgaatcatgc 9900gggtggactt ccctcttgct gttcttgtga tagagttgtt atgagatcca gttatttgaa 9960agtatgtagc atgtccccct tcactctctt gcactcctgc tccaccttgg taagacttgc 10020ttgctacccc tttgccttct gccatgattg taagtttcct gaggcctccc agctatgctt 10080cctgtatggc ctgcagaact gaactgtgag ccaattaaac ctcttttctt cataaattat 10140ccagtcttag gtagttcttt atagcagtgt gagaatggac taatgcagaa ggcatacaac 10200ctttagaatt tgcccccttg agggaacaag atgtgtgaag caggttcatc catagaaaat 10260gtctgagaga acctcaaaat ccctaacctg actaactgat gaaagtgttt ctctcctaag 10320gccagtcagt aaagaccaga gggggtgact gtttctttaa atgcaaaggc agcagcacaa 10380taattcaaga aacatgaaaa atcaagaaaa catgacacca ccagaagaac acaatcattt 10440tccaataacc aactccccaa aaatggagat ttacaaattg gtttataatg aattcagaac 10500aattatgtta aggaagctca gcaaactaaa aggaacacca atagactact ctgtgaagtc 10560aggcaaacaa ttcatgaaca aaactagaaa ttcaaaaaag agaaaaatta tcttaaaaga 10620aaacccagaa gttatggagc taaagaatac aatgcatgaa atgaaggagc gtatcaacag 10680caaagttgat caagcataag aaaaaaaaaa tctgtgaaac tgaagactgg ctatttgaaa 10740ttattcatca gaggattaaa aaaaaaagaa tgaaaagaaa taaagaaagc ctacaggatg 10800tataaaacac catcaagaga actaatataa ggattattgg agtcataaag gagaagagag 10860aaaagggtag aaaacttatt taagaaataa tggctgaaaa ctctccaaat ctaggaaaag 10920atatgagcat ccaggtatat gaagctcaaa gatccccgta caggatacat tccaaaaaga 10980cttcaccaaa acacatgata atcaaactgt caaaagcaaa atcaagacga tgaataaacc 11040accaatcact aagagggaga caggattctt atgttgtcat tattatttac atataatttc 11100agtaaatgtt attggaaaat ttataatgtt ttaaaaaaag aaatttgaaa gcaccgaaag 11160aaaagagact catcacatac agggaaccct tttaaggcat tcaagagatt tctcagtaga 11220aaccttacaa aataggagag agtgggatga actatacaag tgctgcaagg aaaaaaatgc 11280caaccaacgc tttacctggc aaatctgttc ctcagaaatg aaggagagag aagaactttc 11340ctagacaaac aaaagctgag gcagttcatc accactagac ctgccttaca agacatacta 11400aggggagttc ttcaagctga aatgatatgg caatagttag taatatgaaa tgataaacct 11460cactggtaaa ggaaagtaca tagtcaaatt tagaacactt tgatactata atgatggtgt 11520ataaataatt ttactgtgct atgaaggtta aaagacaaaa gtattaaaaa aaacccatag 11580ctgcaatagc ttgtcaatgc atactacagt ataaaaagat gtaaattaga acattaaaaa 11640catagcatgc aagggtaggg aagtaaaagt gtagttttca tatgtaatca aatttaattt 11700gttatcagct taaaataaat tgttatacct atgttttatg taagtgtcat ggtaactata 11760aaggaaaaac ctctagtaga tacacaaaag aaaaagagaa aggaatcaaa acataacact 11820acagaaaatt atcaaattac aaaggaagac agcaagggag gaacaaagta aaaagagcaa 11880gaaaaaattt aacataatga aaacagtaag tccttacgtg tcaataatta ctttaaatgt 11940aaatggatta aattatccaa acaaaaaaac agactggaca aatggatttt agaaacaaca 12000acaacaacaa acaccgcaca cacacacaca cacacacaaa ccacccagcc ccaactatgt 12060gctgcctaca agagatttac ttccacttta aggacacata caggctgaaa ttaaaagaac 12120agaaaaagat attgcatgca gatagaaacc agaagagagg agaggcatct atacttacag 12180catacagaaa agattttaag ttaaaaacta tatcaaaagg ctaagaaggt caaaatggtg 12240aagcagttaa ttgttcaaga agacataaaa attgtaaata tttatacacc caatattgaa 12300gcacctaaat atataaggca aatattaata catataaaag gagaaatata cagcaataca 12360gtaatagtag tgaacttcag tgcctccctt tcaaaaatgg ataatccaga cataaaatca 12420ataaggaaac atttaactta aacttcactt tagaccaaat ggatctaaca gacattatac 12480tgaacatttc atccaacagt ggtagaattc acattcttct caagcacaca tggaacattc 12540tccaggatag attatatgtt agctcacaaa ataatattac aaaatttaat aaagctgaaa 12600tatcaattat tttgcaccac aatagtataa cactagaaat caataacaag atggaaactg 12660gaaatttaca aatatatagc attaacatat tcctgaacaa ccaatgggtc aaagaaaaaa 12720atcaaaataa ttttgtgaca gcaaagtaga aacacaacat accaaaacat acaggacaca 12780gcaaaagcag ttctatgagg taagcttata ttgataaaca catttaaaaa aagattttaa 12840ataaacaaca ttacacctca aggaactaca aggaagaaaa aaaaacaagc cccatgttat 12900caaagggaag gaactaacaa agatcagaca gaaataaatg aaacatagac tagaaaaaca 12960atagagacta ttaataaaac ttagagttag ttttttaaaa aataaaatca acaaaccttt 13020agctagacta aaaaaagaga agactcaaat aaaataaaaa atgaaagagg agacattaca 13080actgatacca cagacataca aattaagaga aaactatatg ccaacatatt agttaacttg 13140caatgggtaa atccctagaa acatacaacc tacaaaaact gaatcatgaa gaaatggaag 13200atctgaacag atcaataatg aataagggaa ttgaatcaat attcaaaaat ctcacaaaaa 13260gaaaagctca ggatcagatg gcttcactgg tgaagactgc caaccattta aaaaaattaa 13320taccactctt tattaagctc ttccaaaaaa attgaagagg agaaaacact ttcaaattca 13380ttataagagg ccagttttac cttgatatca aagatttaaa aagaacactt tgagaaagga 13440aaattacagg ccaaaaccct tgataaatat agatgcaaaa atgctcagca aaatactagc 13500aaacctaatt cagcaacaca ttataatggc atacatcatg accaagtgag attcatgcct 13560cggatgcagg atagttcaat ataatcaaat caacaaatgt tacactactt taacagaatg 13620aaggataaaa atcatatgat catctcgatg gttgaactag tttacagtcc caccaacagt 13680gtaaaaatgt tcctatttct ccacatcctc tgcagcacct gttgtttcct aactttttac 13740agatcaccat tctaactggt gtgagatggt atcttattgt ggttttgatt tgcatttctc 13800tgatggccag tgatggtgag catttttcaa gtgtctgttg gctgcataaa tgtcttcttt 13860tgagacgtgt ctgttcatat ccttcaccta ctttttgatg gggttgtttg tttttttctt 13920gtaaatttgt ttgagttctt tgtagattct ggatattagc cctttgtcag atgagtagat 13980tgcaaaaatt ttctcccatt ctgtaggttg tctgttcact ctgatggtag tttcttttgc 14040tgtgcagaag ctctttagtt taattagacc ccatttgtca attttgtctt ttgttgccat 14100tgcttttggt gttttagaca tgaagacagt gtggtgattc ctcaaggatc tagaactaga 14160aataccattt gacccagcca tcctgttact gggtatatac ccagaggatt ataaatcacg 14220ctgctataag ccataaaaaa tgatgagttc atgtcctttg tagggacatg gatgaagctg 14280gaaaccatca ttctcagcaa actatcacaa ggacaaaaaa ccaaacaccg catgttctca 14340ctcataggtg ggaattgaac aatgagaaca catggaccca ggaaggggaa catcacacac 14400tggggatggt tgtggggtgg ggggagggga gagggatagc attaggagat atacctaatg 14460ctaaatgacg agttaatggg tgcagcacac caacacggca catgtgcaca tatgtaacaa 14520acctgcacgt tgtgcacatg taccctaaaa cttaaagtat aattaaaaaa aataatgctg 14580ctataaagac acgtgcacac gtatgttcat tgcggcacta ttcacaatag caaagacttg 14640gaaccaaccc aaatgtccat caatgataga ctggattaag aaaatgtggc acatatacac 14700catggaatac tatgcagcca taaaaaagat gagttcatgt cctttgtagg gacatggatg 14760aagctggaaa ccatcattct cagcaaacta tcacaaggac aaaaaaccaa acactgcatg 14820ttctcactca taggtgggaa ttgaacaatg agaacacttg gacacaggaa ggggaacatc 14880acacactggg gcctgtcgtg gggtcagggt agggggaggg atagcattag gagatatacc 14940taatgtaaat gacgagttaa tgggtgcagc acaccaacac agcacatgtg tgcacatgta 15000ccctagaact taaagtataa taaaaaataa atcatatcat

catctcagta gatttagaaa 15060agcatttaac aatattcaac atcctttcag aactaaaaac tctcaataaa tcaggtatag 15120aaagaatgtg cctcaacact ataaaagcca catatgacaa acctggaggt aatatactca 15180atggtgaaaa gtaaaaagct ttgactctaa gatcagaacc aaaacaagga tgtccattct 15240caccacttat atttaacata gtagttgaaa ttctagctag agcaattagg caagaaaaaa 15300ggcacccaag ttggaaagaa tgaagttaaa ttgtctctgt agatgacatg atcttatata 15360tagaaaacac taaagactcc accaaaatgc tgttttaatt agagcttaaa aaaataattc 15420actaaagttg caggatacaa aatcagtata caaaaatcag ttgcatttct aaacaccaaa 15480aacaagttat ccaaaaaatt aagaaaacaa tcctatttgt gatatcatca aaaaataaaa 15540tactaaagaa taccaaagaa actgaaaata aatggaaata aatggaaaga tagcccatgt 15600tcatggatta gatgaattaa tactgttaaa atgttcatac tacccaaagc aacctacaga 15660ttaagtgcaa ttcctactaa aattccaatg acatttttca cagaaataga aaacacactc 15720ctaaagtttg catggaaccg caaaagactc aaatagatga agcaattttg agcaagaaca 15780gtaaagctgg agacatcaca ctacctaact tcaaaatttt attaatcaaa acagcatgac 15840ataaaaacag acagaaaaga ccaatggaac agaatagaga gcccagaaat aaactcacgt 15900ttatagagtc aactaatatt caacgaaggt gccaagagta cacaatgggg aaagtatagt 15960ctctacaata aacagcactg ggaagacaat atccaaatgc aaaagaatga aattacaccc 16020ttatcttata ccatacacac aaatcaaatc aaaattgata aagacttaaa tataagacct 16080gaaaccatac aatctttagg caaaaactca ttgacattgg tcttggcaat gatttttttg 16140atatgacacc agaagcacag gcaacaaaag caaacctaaa caagtgggac cctaacaaac 16200taaaaagttt ctgcacagca aaggaaacaa tcaatcatca gaattaaaag gcaatttatg 16260aaatgggaga aaatgtttgc aaaccacata tctgataaag ggttaatatc caaaaatata 16320taaggaatgc ataaaattca atagaaataa acaaacaaat aatccaattt taaaatgggt 16380gaagaacctg aatagacatt ttttcaaaga agacttacag ataggcaaca ggcatatgaa 16440aaaatgcttg acatcactaa taatcaggga aatgcaaatc aaagctccag tgaaatacca 16500cctccaacta ttaggatggc tattatcaaa aactcaaaag aaaacaagct gggggaatgt 16560agagaaaagg gaaaagaaga tccttataca ctgttggtgt gaatttaaac tggaatagcc 16620cttatggaaa acagcatgga ggttcctcaa aaaattaaaa atagaactac tatatgatcc 16680agcaatttca ctattgagta tatatccaaa tgaattaaaa tcactgtctt gaagaggtat 16740ttgcacactc atatttattt cagcattatt cacaatagcc aagacatgga atcaacctaa 16800gtgttcatca gtagatgatt agataaagag aacgtggtat atagacacag tggaatctat 16860ttagtgttca aaaagaagga aatccaactt ttaaaatcct ttaaaaagtt aaactcataa 16920aaacagagag gagaatggcg gtttccagga actggagggt gggagaatgg ggagatgttg 16980gtcaaaaggt acaaactttc agttataaaa tgaataagtt ctagagatct agtgtacaac 17040agcattacta tagttaataa taatattttt tatacttgaa atttgctaag agtaaatatc 17100aatattctca atacacacaa aacagaacta tctgaaggca ctgatatgtt aattatcttc 17160attatagtaa tcatttcaca atgtataatg aatatcaaaa caatagtgta catcttaaat 17220atatacagtt ttgatttgtt aatcatacat caatgaagct agaaaaaatg ttgtaatttt 17280taaaacaata gtaatataaa ttaggggtga aggaattgac ctatgttgga gaaaagtttt 17340tgtaaactat ttaaattaat tggtatccat tcaagctaga ttatttttaa ttgttaattt 17400aattgtaata ctaaggcaac cactaaaaaa gcctttaaaa aaatatagca cttgaggctg 17460ggcatggtgg ccctcaatta tatatataat atatataaaa tatatattat atatatagta 17520gatgaacaac aatgggattt aaatggtaca ctagaaaata tctgtttaac aaaaagaaag 17580caatagtgga gaaatataag aacaaaacca tgtaagattt atagaaaatg aatagcaaat 17640tggttgacct aaaccctatc ttataattat attaaagata aatgaaataa atactacatc 17700aaaaggcaga gattatcaga atagagaaga aaaatccaaa ccataattca accttatgtt 17760atctgtattt agaatattta gagtcaagac acaaatagat agagttccat ttgggcgtga 17820aaatatttac catgcaaaat gtaattaaaa tagagctaga gcagcaatac taatatctga 17880caaaatatac tttaacaaaa attgttgcta aagacaaaaa agaacatttt ataataagac 17940acaacaatta taaacatata caccaaacaa tagagcccaa aatatacaaa gcaaaaactg 18000ctagaattga agaaagatag aaaaatcaat gattacagtt ggaggtgtca ataccaaact 18060ttcagtaata cacagaacaa caagtcaaaa gcaaaaagga aataaaaaac tacacattat 18120catacaacac cttaatacga tatccaacaa tagcagaata cacattattc tgaagtgcac 18180atggaatatt cttcaggatg acacatatta ggctgtaaaa catatcttaa taatttaaaa 18240gaattgaaat aatacagcac atattctctg actgcaaaat attaaaccaa ttacagaaga 18300aaatgtggga aattcacaat gatatggaga tttaaaaata cttcaaagta accaatgaat 18360caaagaacaa atcacaagag aaattagaaa atattttaga tgaataaaac tgaagaaaca 18420acataccaaa attttgtggt tgtagctaaa gcagtgcttc aagggaaatg tataacagta 18480tatccctaaa ttttaaaaaa atctcaatcc aataacctaa tttttcacct taagaaatga 18540gagtgaaaga gcagacttaa cccaaagtaa acagaaggaa gaaataataa agattagcat 18600ggagataagt agtgaaaata aaaacaatag agaaaattaa taaacttgaa agttggttct 18660tctgatatat cagtaaaatt gacgtattat tagtttgacc gagaaagaac aaaagagaga 18720agattcaagt tactagacat aaatgaaagt atagatatca ccttacagaa attaaaagaa 18780ttctaacaga atattgtgaa aaagtgaatg tcaaaaaatt aaattatatg agatacacaa 18840atccctctaa aggcacaaac taccacagct ggtgtaaaaa catgaataca ccatttacag 18900ttaaaaagac tgaatagata aaaattttaa gaagaaattg agtaagtaat ttaattttca 18960aactataatc ccaagaccag atgttttcat tggtgaattc taccaaactt taaaaggatt 19020aatatctatt tttcatacac tctttccaga aaatagaaaa ggagggaaca ctctataact 19080cactgtatga ggtccgtatt acccttatat caagaccaaa catcataaga aaagaagact 19140aaagacttat ggtttctgct ctgatatgtt tggaagtcat cactactatt gtcacaagga 19200aaaatctgaa caaactaaag tcaacgattt cttaaactaa ccatagaatt gaggtaacgg 19260gcgaaaactg gagatgtagg caaatacaaa aaaaatcaca gtttatcagg agcagaaact 19320gctgaaacca gcaactcgta tgaacatgtt aagtggtaat tgacaaattt ctggagattg 19380aatgtggact ggattgagag ttaggaactc ctaagtgccc agtttttgat gaccccacac 19440acttttgtaa actagacttc caagagcccc agcaagtttc ttacagtgaa gactgcagaa 19500aaatcccctg atgcttcaga taggaggaag ggaaaagcaa ctattttgaa ataagcccag 19560gggacaagta gttatttcta aacactctca gagcattttc tttcacacgg cagggggctc 19620cctgcaaggg aagctacttt gcctgagcct tgtctgatgt aggagaaaag gaattggatg 19680gctcaagctc catctagcct ttctgattta tataagggaa gcaaaaaata ggttaagaaa 19740ctcttctgaa agtcacaact cagatttatt ttatatttat ttatttattt atattttttg 19800agacggagtc tcgctctgtt gcccaggctg gagtgcagtg gcgcgatctc ggctcactgc 19860aagctccacc tcccgggttc acgccattct tctgcctcag cctcctgagt agctgggact 19920acaggcgccc agctaatttt ttgtattttt agtagagacg gggtttcacc acgttagcca 19980ggatggtctc gatctcctga cctcgtgatc cacctgcctc tgcctcccaa agtgctggga 20040ttacaggcgt gagccaccgc acctggcctc acaactcaga tttaaccata agattataga 20100acacttctcc tcccaaaaca gagatataga tcaatggaac agaacagagc cctcagaaat 20160aacgccgcat atctacaact atctgatctt tgacaaaccc gagaaaaaca agcaatgggg 20220aaaggattcc ctatttaata aatggtgctg ggaaaactgg ctagccatat gtaaaaagct 20280gaaactggat cccttcctta caccttacac aaaaattaat tcaagatgga ttaaagactt 20340aaacgttagg cctaaaacca taaaaaccct agaagaaaac ctaggcatta ccattcagga 20400cataggcatg ggcaaggact tcatgtctaa aacaccaaaa gcaatggcaa caaaagacaa 20460aattgacaaa cgggatctca ttaaactaaa gagcttctgc acagcaaaag aaactaccat 20520cagagtgaac aggcaaccta caaaattttc acaacctact catctgacaa agggctaata 20580tccagaatct acaatgaact cagacaaatt tacaagaaaa aaacaaacaa cctcatcaaa 20640aagtgggcaa aggatataag cagacacttc tcaaaagaag acatttatgc agccaacaga 20700cacatgaaaa aatgctcatc atcactggcc gtcagagaaa tgcaaatcaa aaccacaatg 20760agataccatc tcacaccagt tagaatggcg atcattaaaa agtcaggaaa caacaggtgc 20820tggagaggat gtggagaaat aggaacactt ttacactgtt ggtgggactg taaactagtt 20880caaccattgt ggaagtcagt gtggcgattc ctcagggatc tagaactaga aataccattt 20940gacccagcca tcccattgct atatatatat atatataccc aaaggactat aaatcatgct 21000gctataaaga catatgcaca ggtatgttta ttgcagcact attcacaata gcaaagactt 21060ggaaccaacc caaatgtcca acaatgatag actggattaa gaaaatgtgg cacatataca 21120ccatggaata ctatgcagcc ataaaaaaga tgagttcatg tcttttgtag ggacatggat 21180gaaattggaa atcatcattc tcagtaaact atcgcaagga caaaaaacca aacactgcat 21240gttctcactc atagatggga attgaacaat gagaacacat ggacacagga aggggaacat 21300cacactctgg ggactgttgt ggggtcgggg gaggggggag ggatagcatt aggagatata 21360cctaatggta aatgacgagt taatgggtgt agcacaccag catggcacat gtatacatat 21420gtaacaaacc tgcacattgt gcacatgtac cctaaaactt aaagtataat aataataaaa 21480aaaggctacc taaaaaaaaa aaaaagaaca cttctcctcc caacaccata tcaccacatc 21540aaccaggact ccagtgtaat agcagtgaat tctaactgaa agaggtgaaa gacactgatt 21600gtatttaaga aagatcttct aaggaaatcc aaaaatagta ggggagatca aaacaaagat 21660actagaggaa attgaatatg tgacacctat agctacaaaa aaattaaaca taacatagcc 21720ctaaccatat aaacataaaa cctcacacaa agacctatta tctgagattc tgttgcctga 21780tacattgcgt tttatttcaa taaaaaaatt agagggtatg ttaaaaagca ggaaaagtta 21840gtctaaagag acaaattgag cctcagaagt aggctcagat atggcagaga tttggcaatt 21900atacctagag tttaatataa atgattaata taataagtgt tctaacagaa aaaggcaaca 21960tgcaagaacg gatgggtaat gtgatcagca agaaggaaac tctaagaaag aagtcaaaag 22020gaaatgctag gaataaaaac ctacaagaaa taaagaatgc ctgtgatggg ttcctcagta 22080gactggacaa ggtcaaagaa tcagtggatt tgaaaatatg tcaacaaaaa ctgccccact 22140gaaatacaaa agaaaaatag aattttaaaa acgtaacaca atctccaaaa cagtgggaca 22200attacaaaag atgtaatgtg cctaatgcaa atgacagtag gagtataaag ggagaaagga 22260atagaaaatc tgaagtaata atggcagaga gttttccaaa attaatgcta aaccacagat 22320acagcaagcc cagagaacaa caaggaggaa atttagtaaa gcgtctgcaa ccaagtatgt 22380catattcaga ctgacaaaac caaaggtgaa gagaaaatat tgaaagaaga caaagaggaa 22440aataaatatc aagaaaatac atacgaaata catcatacat acataagaaa tacatcagac 22500catacaagca agaagagaat ggagtgaaat gtttaaaatg ttgaaaaaaa aactatcaat 22560ttgcaattct gtatccagtg agattatctt tcaaaagtga agagggaaat ggcagagaag 22620tcatctccaa gacctatggt ttcccttcac agaaacactg aaaaatatga acaaaagtgg 22680tcagaattaa ctttctaaga attctataaa atggtaaaat gtttacacca gtaaagcaaa 22740tgctgaattg agaaggcaac ttaaaaaggt gaagaaaact tcgtattatt tttatgtgtc 22800cttgccccac gtccttccct accttagtct tgaagatggc agcccacatt tctactgtgg 22860ggctctggtt tctgtttcct ggttcaagag ggagaataac agaccttact tttagtcatt 22920attatttcct tctttctgat ttccttgggt ttattttgct cttctttcta ctttattgaa 22980atgagaacta agattatgat ttgagacatt tttctaatgt aagcatttag tgctataaat 23040ttccatctca acactgcttt agtcacatcc cacaaatttt tatatgttgt aatttcactt 23100tcatttagtt ctatttttaa attttttctt tttatacttc ctctgactca cagattactt 23160agaattgtgt tgttcagttt tcaaggatat tgtagatttt cctgtttctc tgttgtctaa 23220tagttctgtt ccattttgta cagatagctc acgctgtatg atttcaattt tttaaaaaat 23280tgtgctttgt tttatggccc agatatggtc tgtgctgtga atattccatg ttattataaa 23340gtatgcctgt tatattatta tatatatata atatatataa ttataaagca tgcccgtttt 23400gtattgttag cagagtattc tagaaatgtc aatgagatct tgttggttga tggtgctttt 23460cagttctata tctttgctaa tttttttttt ttttgcttag tagctatatg agattctgag 23520agaggaaatt gaagtctcca accataattg tggatttgtc tatttctcct atcagttcta 23580tcagtttgtg catcacatat ttgaggctct gttgtttggt gcatacacaa gtggaatcat 23640tgtgccctct tggtggctta ttttatgatt atatagtgcc tatctttgtg gtatttttat 23700ttgctcttaa atctactttg tgttatattc atatacccat tcttttttaa aaaaaattgt 23760ttgcgtgata catcttttcc attcttttaa tctcagccta tctgtgccat tgaatttgaa 23820gtgagttttc atatagagaa catattattg aatcatcatt ttaaaaattc cttttgccaa 23880tcttttttat actgaggtaa aattgacata aaatttatca ttttaaagtg tacaatacag 23940tggcatttgg taatacacat gttatgcaac gttaactcta cctggctcct aaatgttttc 24000atcatcccca aaaggaaact tcatactcat taagcagtta attcccattc cttctctcgg 24060ccactggcat ccgcaaacct acttttctgt ctctatgaat ttacctatta tggatatttt 24120gtataaattg aattatacaa taagtgacct ttatgtttgg cttcgttcgc ttcgcatact 24180atttttcgat attcaaccat gttgtagtat gtatcagttt tatttgaata actcaattct 24240ttttgttgta tagctaaaag ttgattccta ggtcataatg ataattctat gtttagttta 24300ttgaatagct gccaaagttt ttccacagtg gctctgtcat tttaaaatcc cactagcaat 24360ggatgagagt tccaatatct ccacatcctt accaatattg ttattttata tttttataat 24420tataattttc ctagtgaata cgcaatggta tctcattgtg tttttggttt gcctttccct 24480aatgactaat gatgttgagc atcttacaat gtacttgtta actatttgtg ttctttagag 24540aaatgtctat tcaagtgcct tgtccatttt aaaaatcgag ttgtcttgtt gacttatgag 24600ttctttaata cagtaaacgc ttatcagata tgatttataa gtattttaac ccattctgaa 24660ggtcaccttt tcacttttgt ggtagaccat tatgcacaaa ggttttaatt ttgataaatc 24720caatttatcc gcttttgttg ttgtttttgt tgttcgtgct tttgcaaaac ctagtgtcat 24780gaggttttct cattatcttt ggagaatttt atagtttggg tctatacatg tagattattg 24840atctaatttc cattaatttg tgtgtatgct acgaggtagg ggtccaaatt caatttttgc 24900attgaattga aaattcatat tttcagtttc aaatttcaac tgcatattca gttgttgcag 24960cacaatttgt tgaagagatc attctttacc acagggaatg atctgggacc cttgtcaaaa 25020atcaattgat catagatgta tgggattatt tcagacttta gatcttgttc catgaatatg 25080cctattttta tgccagcact gcagtatttt cattactgta gctttatcat aaattttgaa 25140atcaggaagt atgtatcctc caagtgtgat tttcatttgc tagagtgttt tgactatttg 25200gggtctttgc aattccatat gaatttcaga attggctttt catttaaaaa aatggtagtt 25260gagattttca taggaattat attgaatcta cagatcactt tgggtagtat tgccatctta 25320acaatattgt attccaatcc ataaacacgg atgtattgcc attcatatct ttttttcttt 25380tctttcggca acatttagta tatgacactt gtaactcctt ggttaaattt atacctaagc 25440attttatcct ttctgatgct gtttaaatgg aattatttta ttaatttctc ttttagatgg 25500cttgttgcag gtgtatagaa atagaactga ttattgtgct tttattgaat tatctgaaac 25560tttgctgcat ttattaactc tagtaggttt tttttttctt taaagttgtc tatatatctt 25620gctctgtgaa tagataattt tacttcttga tctccaatat ggatgccttt ctttcttttt 25680cttacctaat tgctgtagct agaattttca gtataatgta tgatagaagt tgttcacaac 25740tatccttgtc ttctttctga tcctaggggt atagctttca gtctttcacc attaagcaca 25800atgttagctg tggggttttc ttagatgcca tttaatatat taaggaagtg ctctgctatt 25860tctaattggt tgagtatttt tattataaaa tggtgttaga ttttatgact ttatgtactg 25920cataattgag attatcatgt ggtcttttca ttcgattaat gtggtatatt ttatgatttt 25980catatgttga tccaccttta tattcttggg gcaaatccca ctgtgtacac gggtttttta 26040gggtctccta attttcttca ttctttttct tttctctccc tgagactgaa taacgttaac 26100tgacctatct tcaagtttac tattttttct tctgctgttc aaatctgctt gaacctatag 26160agtgaatttt tcattttact tattgaactt ttcagctcca aaatttctct ttggctactt 26220tgtataatat ctatctctct atagatattc tctatttgga aagacatttt tctcctggtt 26280ttctttactt atttgtattt tttaaagtct ttaagcatat ttgagacagt tgatttaagt 26340atttgtgtac taagtccatt gcctaagctt ttgcatagag attttatatt aatttctttt 26400ttttctgtga acaggtcttt tttttttttt tttttttttt ttttttttta gaaaactgga 26460cattttgagt agtataaacg tggctatttt agaaataatt ttcctccctc cttaggtttt 26520gtttccactt gttgcatgtt gctgttgttt gctcattagt gacttttcta aagtattttg 26580aaaagtctgt gttcttgatc atgtgggtcc attgaattct gtattctgtt aatttcatag 26640tcagctagtg ttctgaaagt tcccttaagt gcatagagcc aataaaagaa aaagaaaaga 26700aacacagaaa aagaaaagag gaaaaaagga gggaaagaga tttaaaaaaa taatgtcggt 26760tgggcatggt ggctcatgcc tgtaatccca gcacttttgg gaggccgagg caggcaaatc 26820acttaaggtt gaccatcctg gccaacacgg agaaaccttg tctctactga aaatataaaa 26880ttagccgggc atggtggcac atgcctgtaa tcccagctac tcgggaggct gagaggtagg 26940agaatcactt gaactcggga ggtggaggtt gcagtgaact gagatcacac cactgcactc 27000cagcctgggc aacaagaatg aaactccatc tcaaaaaaaa ataataataa taatgccttt 27060gccgatttgc tctgtgtttg ggccctcatt caatgcctag tcaggccatt tacaactctc 27120tcttaacttt cactacctgc ttgtgtactg actgaaggcc acctataggt gaaagcttaa 27180catcatctca gatctttttg gaccatgaat cctaccctgg gtatgcacat gatcttctta 27240atttcccagt agatgcaaga gttttagtgt tttaaaagtc cttattccct catctatctt 27300cttttctgac ctttttcagt ctgcttattg ttcatctgaa ctgacatcct ttgccccaag 27360cggctgtggc aaaaactttt acctttaaat gctttcacca caagccactt gggaagctgc 27420cccagacctg ggactgctct gaccctgatg aaacaaagac aagaccttgt acagccaggc 27480agccataaga caagtccata cccaaaccac agttctttca gaataaggtc tatattggat 27540tctctggccc tagtaaccag catgagtctg ggcttgccat cttcatggcc acttgtctta 27600gtttgctagg gctgccataa caaaatatga gagactgagt ggcttaaaca agagaaattt 27660attttttcac aattctgaag accagaagtc cattactctg aaatctctct ccttggcttg 27720cagatactgc cttcttgccg tgtcctcaca cagccttttc tctgtgtaca catccctggt 27780gttttttaag cctgtccaaa ttttctgttc ttctgaggac accaatcaga ttggattagc 27840gaccacccat atgatcattt taccttaagt acctcttcaa aggtatcaga gtagtatatt 27900tgctacagtt gatgaacctg catacaacat catcacttga ggtctgcagt ttacattaga 27960gttcatcgtt agtgttatat attctagggt ttgttttgtt ttgagacaga acagagtctt 28020gctgtgttac ccaggctgga gcgcagtggt acaatctcat tgctacctct gcttcccaga 28080ttcaagcaat tctcatacct cagcctccta agtagctgga actacaggcg cacaccacca 28140tgcccagcta attttcgtat ttttagtgga gatggggttt caccatgttg gccaggcagg 28200tcttgaactc cttgcctcaa gtgatctgcc cgcctcagcc tcccccagtg ttgggataac 28260aggcatgagt caccatgcct ggccttattc tatgggtttt gaaatgtata atgacatgta 28320tccatcgttg tagtattaga tagaatagct tcattaccct aaaagtcttc tttgcactgg 28380gttgagtttt aaaagctctt tgattatttt atgacagttc cttattagat atatcttttg 28440caagtatttt tatcagtctg tggttatctt gtcttttaca gagcagtaat ttttaatttt 28500aataaaatcc aatttgtcaa ttacttatct catatgactc tggtgttaca tctaaaatgt 28560taccaccata ctcaaggtca cctaggtttt ctctaggaat tttatggttt tgcattttac 28620atttggtgta tgacccattt gaagttaatt tttgtgaggt tgtaaggtct gtgcctagat 28680tcattttttt tttttttggc atgttacttc agtattcata agaaacattg ttctgtaatc 28740ttctttctta tagtatctta gtcttgcttt agtttttggg caatgctggc ctcactgaat 28800aaattcaaag tgttccctcc tcttcaatta tttggaaaag tttgagaaag actattgtta 28860actgttttct aaatttttgg tggaatttac cagtgaacca actggtccta ggcttttctc 28920cagtaggtgg ttttgattat gctttcaatc tctttacaag ttacacatct atacagactt 28980ttataattca gtcttggtag gttgtgcgta tttaggaatc tgaccacttc atctaagtta 29040tccaattagt tggcatgcaa ttattcgtag ttctctgaat aatcattttt atttccacaa 29100aattggtaat atcccagttt ccatttttta tttcattgaa tcttcttttt tcttagctaa 29160tctagctaaa tgtttgccaa ttttgttgat cttttggaag aaccaacttt tgatttatta 29220attttctcta ctctttttct gttctttata ttatttattt ccacactaat ctttactatt 29280ttcttccttc tgttggcctt taattttttt tttttaattt ttaaggtgta aatttaggtt 29340gagaaatttt ttaaatgaaa gcatttagag ctataaattt tccttctggt gttcctttca 29400ctacctgcca taaattttga tatgttgagt ttttgtttgt cttggagtat tttctaattt 29460gtcttctaat ttcttccttg acctattggt tatttaaatg tatttaattt ttgcatattg 29520tggatttccc agttttcctt ctgttattga tttctagttt tattccattg tgatcacaga 29580agatattttg tataatcgca gtctattaac atttattaag tcttgtggcc taacagagga 29640tctatgttgg agaatgttcc aagtgcaatt gagaatacta ttctggtgct attaggtgaa 29700gtattctcta tatgtctgtt aagtccaatt catctatagt gttgacgttt cctgttcctt 29760actgattttc tgacttatta ttctatccat tattaaaagt ggagtggtaa agtctctatt 29820attgtagaac tctctgtttt tcaagtctat caatatctgt ttcatatatt ttggagctct 29880gtttgctgca tatgtgttta caattgttat atcttcttgg caaattgacc agtttcatca 29940acataaaata taattcttat tgtcttctaa cagtttattt ttctttttac ataaagccta 30000ttttatctga tcttagattc cctcacactc caccccagca cgcttttggt tacatttaca 30060taatatatct tttccatcct ttcattttca acctgtttgt

gtctttagat ctaaagtgaa 30120tgtcttacag acagcataag ctatgtcatt aaaaaaatcc attctgctta tctctgcctt 30180ttgactgggg agtttaatcc atttgcattt aaagtaatca ctgatcatta aatactttca 30240gtattttgtt gttttatgta tgtcttataa ctcttttgct cttcatttcc ttcaatattg 30300cctttgtgtt tagcttatct ttttgtgtca cactttgatc cccttctcat ttctttttta 30360tattttcttt gtggttacca ggaggacaat gtatcaactt ttaaagttat tacaatttta 30420tttttttaaa tctctcccat tcggggattt taggaaggtt aaataataat gtaaatgaga 30480tacctagaac aatataagca ttcaggaatt attaactcaa ttccaatcct tcctccacct 30540ccacctcttt ctctgtgaga ttatagaaaa gatgacaaaa aggatgtttt ctgagccctt 30600taattgttga gaatgatctt tgagaaaaag aaaaaaaatg aaagcactag gaatgtacaa 30660cagcctggaa gtataattaa gtgtaaatta aatagataaa agttataagc agaggaaagt 30720atagtagaac tcagtattta aaagagaatc aatgtgaaaa ttatataaat ttatgtaaaa 30780taaaactacc agacaaatct gatatcctta ggatttttct ttctttcatg tgatttctaa 30840ttgctacata tgacactaaa ccattgatct gagctgtaag agaaactgga aattgttctg 30900ttatcttttg taagatttct agaacatttt gccctcagac ttaaatgcca acgtatttct 30960cacttattgt ttactgcttt tggatttaca tatgatttga ttctttctta tctcttatcc 31020ttacaatgta attcaaactg atgccaattt aagttcaatt gcgtacaaaa actctactcc 31080tatgcagctc cgccccatct aatttacatt attgatgtcg caaattccat ctttgtacat 31140agtttacata ttaacatgga tttatacatt tttatgtatt tggtttttaa atcctgtaga 31200aaataaaaag tcaacacacc aatattaaaa taatactggt ttttatattt gtccatgtgc 31260ttacctttat cagtgttctt tacatcttta tacgggtttg agttactgtc ttgtgtcctt 31320tagttccaac ctaaagaact ccctttagca tttttatagg gcaggtctag tggtaatgaa 31380ctctctgagt ttttatttag ggatgtctta atttctagct cctgtttgaa gtaaattttt 31440ctggatatac aattctctgt tgattgattt ttgttcattt tcccttcagc tcttttaaat 31500acattatccc actttcttct gccttccaag gtttctatta acaaaattca gcttataatc 31560ttattaaaga tctcatgtac atgagtggct tctctcttgc tgttttcaag attctgtgac 31620tttggtttct gatagtttaa atataatgtg tcttattgtg ggtctctttg gatttatcct 31680agagtttctt ggtcttctta cgttggtata tccatgtatt tcaagacatt tgagtagttt 31740tcagccatta tttcttcaaa caatctctcc tctttgggga cttccatttt acctatattg 31800gttcttttga tggtgtggca ccagtcccct agactttgtt cacttttttc cagtctttta 31860tttctgcttc tcagactcaa cagcttcaag tgttctgtat tcaagtctgc tgactctttc 31920ttcttccagc tcaaatctgc tgttggatcc ccccttgtaa aatttttaat tccattttag 31980tgtttttcaa gttaagtatt tttattaggt tcctttttat aatttctttt tgttgatatt 32040ctcattttat tacacataat ttgtctgatt tccattagtt tttttctttg ttttccttta 32100gctctttgaa aatatttaag acattttaaa agtctttatc caagttcaat ttctatggtt 32160ctgtagagat attttctgcc agtttatgtt cttcttttcc atgggccatg ttttcctgtt 32220tctttgtata ctttctaatt tttggttgaa aactgagcat ttgaaaatag agccaacttt 32280cccagtttct gcagagagtc tttatgccac agtattcgtt cactgattaa ctgggtatat 32340ctaagcttag ggagcagctg agtcaaaagt ttaaggtctt ctcaggtctt ttctgagtat 32400acatgtttcc tatgcctgtg tgaaatgttc tcaatttccc tatataaaca gctacttctt 32460cttttttttt tttttttgag atggagtctc gctctgtcac ccaggctgga gtgcagtcat 32520ctcagctcac tgcatcctcc acctcccagg ttcaaacaat tctcctatac aggtgtgtgc 32580caccacgtct ggccaatttt tgtattttta gtagagacag ggtttcgccg tgttggccag 32640gctggtctca atctcctgac ctcaggagga ttacaggctt gagccacagt gtccagccta 32700aacagctact tttgaatgct ttaatttcct gaatagtctc aacccagttt ttccttgagg 32760tcttaggtgg tccattgtat gtctccaccc atagttgctt gccccaggca tctgtgggtc 32820tgtggtacca ctgcagcttt caccacctgt agctgccacc tttccctatc tgagatccag 32880gttaggtgag agagatcatt ccttcacgca gtcccatgac aggttggaac atttcaaata 32940aggtctgttc tgctcctctg gttgaaggga gaaaattggg aaccggtttc ccaccttcta 33000caaaccaaga tctcatgttg ccacgggagt ggcagggcaa gtgcaagtga aaatgccata 33060caattttcta ccattttgaa cgcgggtttt tcttcaatgg tcatttgctt ggttgctgta 33120ggcctttcac tgttttccag agctcccata agattacttt agccagtttt ttgttctttc 33180ctgatgcttc cctggcagag taagggttgg aacttccacc attttgctga ttcataactc 33240tgtagtcagt tttaaatata ttgatacttg agtttgtttt atggcccaga atatggtctt 33300ggtaaatgtt tcacatatac tcagaaagaa tgtgtattct gatgttgtta catgggctgt 33360tctataaatg ttacttaagg tggttaataa tgttgctcaa gtcttctata ttcttgctga 33420ttttctttta tttatttatt ttattttttt tattatactt ttaagttcta gggtacatgt 33480gcacaacatg caggtttgtt atatatgtat acatgtgcca tgttggtgtg ctgcatccat 33540taactcatca tttacattag gtatatctcc taatgctgtc cctccctgct ccccccaccc 33600catgacaggc cccagtgtgt gatgttcccc tttcctgtgt ccaagcgttc tcattgttca 33660attcccacct atgagtgaga acttgcggtg tttgtttttt tgtccttgtg atagtttgct 33720gagaatgatg gtttccagct tcatccatgt ccctacaaag gacgtgaact catccttttt 33780tatgactgca tagtattcca tggtgtatat gtgctacatt ttcttaatcc agtctatcat 33840tgatggacat ttgggttggt tccaagtctt tgctattgtg aacagtgctg caatgaacat 33900acgtgtgcat gtgtctttat agcagcatga tttataatcc tttgagtata tactcagtaa 33960tgggatgggt gggtcaaatg gtatttctag ttctagatct tgaggaatca ccacactgtc 34020ttccacaatg gttgaactag tttacagtcc caccaacagt gtaaaagtgt tcctatttct 34080tcacatcctc tccagcacct gttgtttcct gactttttaa tgatcgccat tctaactggt 34140gtgagatggg atctcattgt ggttttgatc tgcatttttc tgatggccag tgatgatgag 34200cattttttca tgtgtctttg gctgcataaa tgtcttcttt tgagaagtgt ctgttcatat 34260cctttgccca ctttttgatg gggttgtttt gttcttgtat atttgtttga gttctttgta 34320gattctggat attagccctt tgtcagatga ggagattgca aaaattttct cccattctgt 34380aggttggctg ttcactctga tggtagtttc ttttgctgtg cagaagctct ttagtttaat 34440gagaccccat ttgtcaattt tggcttttgt tgccattgct tttggtgttt tagacatgaa 34500gtcctcgccc atgcctatgt cctgaatggt attgcctagg ttttcttcta gggtttttta 34560tggttttagg tctaacattt aagtctttaa tccatcttga attaattttt gtataaggtg 34620taaggaaggg atccagtttc agctttttac atatggctag ccagttttcc cagcaccatt 34680tattaaatag ggaatccttt ccccatttct tgtttttgtc aggtttgtca aagatcagat 34740ggttgtagat gtgtggtatt atttctgagg gctctgttct gttccattgg tttatatctg 34800ttttggtacc agtaccatgc tgttttggtt actgtagcct cgtagtatag tttgaagtca 34860ggtagtatga tgcctccaga tttgtccttt tggcttagga ttgtcttggc aatacaggct 34920cttttttggt tccatatgaa ttttaaagta gttttttcca attctgtgaa ggaagtcatt 34980ggtaacttaa tggggatggc attgaatcta taaattacct tgggcagtat ggccattttc 35040acgatactga ttcttcctat ccatgagcac ggaatgttct tccatttgtt tgtgtcctct 35100tctatttcgt tgagcagtgg tttgtatttc tgcttgaaga ggtccttcac gtcccttgta 35160agttggattc ctaggtattt tgttctcttt gacgcaactg tgaatgggag ttcactcatg 35220atttggctct ctgttagtct gttactggtg tataagaatg cttgtgattt ttgcacattg 35280attttgtatc ctgagacttt gctgaagttg cttatcagct taaggagatt ttgggctgag 35340acgatggggt tttctaaata tacaatcatg tcatctgcaa acagggacaa tttgacttcc 35400tcttttccta attgaatacc ctttatttct ttctcctgcc tgattgccct ggccagaact 35460tccaacacta tgttgaatag gagcggtgag agagggcatt cctgtcttgt gccagttttc 35520aaagggaatg cttccagttt ttgcccattc agtatgacat tggctgtggg tttgtcataa 35580atagctctta ttattttgag atatgtccca tcaataccta atttattgag agtttttagc 35640atgaagggct gctgaatttt gtcgaaggcc ttttctgcat ctattgagat aaacatatgg 35700tttttgtctt tggttctgtt tatatgatgg attatgttta ttgatttgtg tatgttgaac 35760cagccttgca acccagggat gaagcccact tgatcatggt ggataagctt tttgatgtgc 35820tgctggattc agtttgccag tattttactg aggatttttg catcaatgtt catcagggaa 35880attggtctaa aattctcttt ttttgttgtg tctctgccag gctttggtat caggatgatg 35940ctggcctcat aaaatgagtt agggaggatt ctctcttttt cctatttact gtacatttat 36000tccaccagtg actaaaacag gtgtatcaat aaaatctgtt ccctcaggtt ttgcttcagg 36060tattttgagg gtctgttatc aggtgcataa acaagattgt tatgtcctat tcttaaatta 36120atctccttat aattatgaag ttaatttttt ttttcttgag atgcagtttt gctctgtcgc 36180ccaggctgga gtgcagtggc acaatctcgg ctcagtgcaa cctctgcctc ctgggttcaa 36240gcatttcttt gcctcagcct cccgagtagc tggggttaca ggtacctgcc accacgcccg 36300gctaattttt ttgtattttt agtagagatg gggtttcacc atcttggcca agctggtctt 36360gaactcctga actcttgatc cacccacctt ggcctcccaa ggtgctggga ttacaggtgt 36420gagccactgc gcctggacct ggcccgaagt aaacttcttt acccttgcta atgatctttg 36480ctctgaagca tgctttgctg gtattaatat agtcattcct tctttctttg attcatgttt 36540gcagggtata tctgtttcca ttcttttact tttaacctat tgtctttata tttaaagtgc 36600atttcttgta agtataattg gtttcttaaa atccaattat ctgcctttta aatgtcattt 36660ttatatgatt tgcataaata tgattattat tacagctaaa ttgaaatctg tcatcttgct 36720atttggtttc tatttatccc atttttttcc cctctttttt tgctttcctt gagattgaac 36780attgtattag ttttctaggg ttgctgtaag aaagtgacat aaagtggatg gcttaaaaca 36840acagaaattt attgtttcag tttggaggct agtcatctga aaccaaggtg tcatcagggc 36900cgtattctct ctgaaacctg tagggaagaa ttctttcctg cctgttctag cttctagcat 36960tttccagcaa ttcttggcat tcctttgctt gtagatgtat ccctccaatc tctgcctcta 37020tcataacata gccatcttct ccctttatct gtctattctt ctcatcttat aagaacatta 37080attactgaat tggggcccag catagattag gcctaatctc atcttgaata ggttacatct 37140gccaaagatt cttcttccaa ataagatcac ttttacagct tttacaggta ctgagagtta 37200gaacctcaat atatcctttg gtggggaccg acctcttacc cataaaaagt attttatatg 37260attccatttt atctcctttt taggttatta actacaattt ttttttcttt tttttgagat 37320ggagttttgc tcttgtagcc caggctggag cgcgattttg gctcactgaa acctctgcct 37380cccgggttca agtgattgtc ctgcctcagc ctcctgagta gctgggatta caggcatgtg 37440ccaccgtgcc tggccaattt ttgtattttt agtagaaaca gggtttcacc atgttggcta 37500gggtgggtct caaattcctg accttaggtg atccacccac ctcggcctcc caaagtgctg 37560ggattacagg cgagagccac cacgcctggc tttataattt ttttttaatt cagtggatgt 37620tttagggttt atagtataca tctttatcac agtctagctc caagtgatat atccctttat 37680gtatagtaca tgacccttac agtagtgcat ttccattttt cctctctggc atttaggcta 37740ttgcacacac atacactcaa ttcatccctt tgtgtagatc cgtatttcca gctgctatct 37800tttgcttctg tctgaaatat atgtatgatt tcttttatga ctattttgag aaatttggtt 37860atgtgcattt gtctattttt cttatgtact tttagctcat caatcttaag tctgtgagtt 37920tatagttttt aaaaacaaat ttgaaattat ttggctatta tttcctcaaa tatttttttc 37980tgccgcccct gctttccctt tccttaggga tctctgattt ccacctatat tactctgatt 38040gaagttgttc cacgtctctt ttgaaaatct ctgaaaaatc ttttatcatt tggataatct 38100gtatttgtac atcttcaagt acattaatat tttcttttgc aatgtttaat ctgctgttaa 38160tcccatgtag tggattcttc atctcaggta ttgctgtttt aatctctaga aattccattg 38220aagtcttact tgatgaaaaa ggcagataaa aacaaatgtt ggcaaagata tggagaaatc 38280agaatctgca cacactgatg atgggaatgt aaaatggtca aggcaatttg gaaagcagtc 38340tggcagtttc tcaaaaggct aaacgtagtt cccatatgac agcaatcatt catctaggta 38400tatactccag agaaataaaa acatatccac accagaactt gaacattaat tttcatagca 38460acattattcc taggggttta aaatttttta cattattttt atttaaaata gagacagggt 38520ctcactacgt tgcataggct ggtctggaac tcctggaatt aagcattcct cctgcctttg 38580tcctgttttc tccactggaa aagaataaaa ctttgtacac ttggactaac aactcccgat 38640tccctccttt accaacatgc cccacagcct ctagtaactt actctctact ttcatgaatt 38700caactttttt aagattccac atataagtga gatcatacaa tatttgtctt tctgtgcctg 38760gcttatttct cttagcataa tgtcctccag attcatacat gttgtcttaa atgacaggat 38820ttaccttcct ttaaaggctg actagtactt cattgtgtat atgtatcaca ttttctttat 38880ccacttatct gttgatgggc acttaaattg tttcaatgtc ttggctactg taagtaatgc 38940ttcaataaac atgggaatga agatatccct tcaacatatt gatttctgtt cttttggata 39000atactcagaa gtgagattac tggatcatat ggttgttcta tttttttcag aaacctccat 39060actgtttttc atagcggctg tactaattta cattcccact aacaatgcat gagttcactt 39120ttctggacat cctccccaac acttgttatc tttcatcttt ttcataaaag ccattatata 39180ataggtgtga agtgacatct cactgtggtt ttgatttgca ttactctaat aattagtgtg 39240agcatttttt ttttttcatg taccgaatgt cttttgagaa aggtctcttc attcctttgc 39300ccattttaaa atcaggtggt tttcttgctc ttgagttgtt tgagttcctt atgtatttta 39360gatatttacc catttccaga tatatcattt atattttttc ctattctttg agttccctct 39420tcactgtgtt gtttccattg ctgtgcaggt cttttatttt gatgccaccc catttgtcta 39480tttttgccat gcttttgcag tcatatccaa aaaaatcatt cccaagacca atgctgtgga 39540gatttccccc tatgttttct tcagtaggtg tacagtttta ggtcttatat gttaagtttt 39600aaatctattt ttttatatgg tgtaaataag ggtctaattt aattcttttg catgtggata 39660tccagttttc ccaacaccat ttattgaaga ccctgtcctt ttatactttt cagtatgcag 39720atcttttacc tccttaaatt tacacctcag tatttaatat ttgttgctat tatgagattt 39780tcataatttc cttttcagat agctcattaa tagtagatgg aaacactact gatttctgta 39840agatgatttt gtattacgga actttactga gtttgtgtat cagttctacc aggttttagt 39900tttgttctgg tggagacatt acagtttttt gtatatggtt atgtcatcag taattacaga 39960tgatttaacc tattcctttc ctattaggat gccttttttt tctttctctt gtccaactgc 40020tctggttagg acttctagta ctatgtcaaa aagtgatgag ggtcgtacat ggcctccata 40080cctaatctgt tgagagtttt taccatgaaa ccaggttgaa ttttgtcaaa tgctttttct 40140gcatccattg agatgatcat atgatttgat ttacaccctc cattttgtta tgtggtatat 40200cacacttttt gatgtgcata tgttgaacca cccttgcatc ctaaggataa atcccacttc 40260atcatggtga atcattcttt gtattcgtga atccagtttg ctaatatatt gttgaggatt 40320tttgcatcca tgttcatcag ggatattact ttgtaagttt ctgtccttaa agtgtctttc 40380tctggcttta ataacagtgt aacactaccc ttgtaaaatg aatttagaag tattccctct 40440gcttcattgt tttggaaaag tttgagaatt tttattagtt ctttaaatgt ctggtaaaat 40500tcagtagtga agctgcctaa tcctgggctt tcctttggtg ggatactttt tattactggc 40560tcaatctctt ttcttgttat tggcttattc agatttgttt cttcatgatt cactctttgt 40620aggttgtata tgtctaggaa tttattcatt tctttaggtc atccaatttg atggtgcata 40680acttcatagt agtttcttat aatcctttgt attttggtga tatcagtagt aaatgtctcc 40740tctttcattt ctgatcttat ttgagtactc tttttttctc ctagtctagg taagaatttg 40800ttgattttat ctttcaaaaa aaaaaaaaac caactcttag caactcttag ttttgttcat 40860ttttttccag tctttatttc aactgtgatc tttgttactt acttctttat gctaactttc 40920gggcttagtc tgttcttttc ctagttcctt taggtgaaaa gtgagattgt gatccttctt 40980ctttattggc gtaggtttgt atcgctataa atttccatta ggactgattt tgctgcatca 41040cataagtttt gtttccattt tcatttgtct caaggtaatt ttttatttac tttttgactt 41100cttctgtgaa ctattagttg tttgggagca tattgtttaa tttccacata ttgctgtatt 41160ttccaccaga attgattctt gttcttgatt tctagtttca cgccattgta atcagaaaag 41220ggatttgata tgatttctgt ccacttaaac ttaagattag ttttgtggac taacatatat 41280cctggagaat gttccatggg catttgagaa caaaatgtat tttgctgctt ctggatggaa 41340tgtttcatat atgcctgtta agtccgtttg gtctaaagtg taattgaaat ccattgtttc 41400tttattgatt ttctgtctag gtgatcaatc tgcccatggt gaaaagtaga gtattgaggt 41460cccgtattat agtattgcag cctatctccc tcttcacatc atttaaaaat tgctttatgt 41520atttaggtgg gtcaatgttg ggtgcatata cttttacaat tgttatgtct tcttggtgaa 41580ttaatccctt tatcattata taacaaactt ctttcttttt atagtattga cttaaagtct 41640attttgtctg atagaagtat agctacccct gctctcaatt tccatttata tagaatatct 41700ttttccatcc cttcactttc agtctatgtg tatgtttagt agaaaagtga atctcttgcc 41760gggcgcagtg gctcactcct gtaatcccag cactttggga ggccaaggcg gcgggatcac 41820cagaggttgg gagttagaga ccagcctgac caacatggag aaaacctgtc tctattaaaa 41880atacaaaatt agccaggcgt ggttgtgggc ccttgtaatc ccagctactc gagaggctga 41940ggcaggagaa ttgcttaaac ccgggaggtg gaggttgcgg tgagctgaga tcatgccatt 42000gcactccagc cttgacaata gcaaaactcc gtctcaaaaa aagaaaaaga aaaagaaaaa 42060aaagtgagtc tcttataggc agtatgtggt tgtgacttaa aaaaaaaaaa aaatccattc 42120tgtcattcta tgtctttttg ttggagaatc taatccgttt acattcaagg taactattgg 42180taagaaactg ctagtgtcat tttgtaattt gttttctgat tgttttgtag gtcccttgtt 42240tcttttttcc ctattgctac cttcctttgt ggtttggtgg ttttctgtgg tggtgtgctt 42300tgaatccttt cttttatagt atatgcgatt accattgtat ttgctgtaag gctaacttaa 42360aacattttat ccttaggcta ctttaagcga taaaaacttg cctttagttg catacaaaaa 42420ctctacgctt tcacaacccc cccgcctttc gtgattttga tgtcaaagtt tacacttttt 42480taaatttgta tctcttattg tagctacagt tacaattact tcttgtagct acaacttatt 42540gtagctacag ttgtttttaa tagtttcatc ttttaaacct cctaatagga ataacattgc 42600tttacctgcc acctttacaa tactagagaa ttctgactat gaattactta taccatttag 42660ttttttactt ttatgtttct cattaactag cagtctttta tttaagccta aagaactccc 42720ttttagtaat tccagtagag caggcctagt agtgacaaac tcccttaggc attgtttatc 42780tggaaaagtg tttatttctc cctttgccaa gtaaagtatt cttaattaac agctttgttt 42840ccttcagcac tttgaatata ccatcccact ctctactggc ctgtaaggct tttgctgata 42900aagccactga atctgtattg gggctacctt gaatgtgatg tttcttatct ttgctgcttt 42960cagtattctt tgtctttgat ttttgataac ttggttgtga tgtgtcttgg tgaactcttt 43020aggttgaatc tgatcggtga cctcagcttc ctgttcctga attttgtcat cttttctcag 43080atttgggaat ttttcagcta ttacttcctt aaatatgctt tctaggcctt tttctttctt 43140ttctccttaa ggacctccta ttatgcaaaa gttagctagc ttgatgtgtc ctgtaattct 43200tataggcatt cttttttgtt tttgtttctc attggataat ttcaaatgtc ttacctttga 43260gctcattgat tcttctgctt gatcaagtct gctgttgaag cgttctactt agattttcag 43320ttcagttact gtattcttta tctttagaat ttctattgtt tttgtttata tttgttaaac 43380ttctcattct gttcagatgt tgttttccaa atttcatttt tctatccata ttttcttgta 43440ctttgttgaa cttaagagga ttagtctaaa ttatttgtca tttcacaggt ctccatttct 43500tctgagtctg ttactggagc tttcatatcc aatgtacacc agaaactttg ctaattccac 43560agtaaatgtt agaaatgggt ttttattgat aaaatcagtg gcttgctaat tagtatgtta 43620gaagctggct tgcattggtg aagttgaggg tattcgatat acgtatcccg gctaaaatac 43680ttctaaagaa cctctccagc tttgcaagaa tagagtatag gaatctgagg aggcctttaa 43740catggccctc agaaaggaca cacagggctt gacctctaaa catataaaca tatacagaaa 43800cgaggtcaga acatgttttg actgatgcca gtagtaacac agcagagcct attgctggtc 43860actgttacca ataccttgtg taataaacct ttcatttaaa ttgagaggtt gtaagccccg 43920aaaaagcaaa gggcttacag tacctcttgc ctgaacactc ctgtgtgtgc tttctaagaa 43980atgcttttaa aataatgcct gtggcacaaa ttgatttgac actggctctc tttataagct 44040gctatcaagg ttgtggggaa tagctaactc ttacccactt tccctgacaa ataggaaccc 44100gtagttacct accaaaagta tgtaaaagct atatccgacc caaaggccag ttaggtcaaa 44160acaccttgaa aggagctcag tttaaggaaa tcagtggtgt cacagtgccc cactagcgtg 44220taaaagtttt catagtttga ctatcggatg tgcttagttt cacagctgag tcttaccttg 44280tgatactttt agagcaaaag tcaaatcaaa gatgatttaa aaaaacattt caaagattca 44340taaagaataa atgcctcctc aggaagtctt ctggagttct gcccacttcc cttaggtggc 44400tctttactgt gcccatctca tagcctgtct ccttccactt ggtgtattgc agagtaaagc 44460tcactgttta caggggtggt agaaagtgtg gtcctttccc agactccttt ttcccttcct 44520cttctcattt ttcagaagat gtgtttgata ataaacgaaa caaaatgact aacacattga 44580gctgagctac ataagcagat gtcagtttga cgtgaagagt ttaaaagatc tatgcattat 44640ctggggactc ctcccccaga cctgaaggat caggtgctgc cttctatgcc acctgtgcag 44700acagcaaaag aggaaaacca tacccacgtt cagtatgaac aaaggggaca tttgaactct 44760gtgtggaccc ctcattggaa gggtgtttct tctcctgctg catccacaaa gagcactcct 44820tagccttgcc ttttgtcagt tctctctcca ataaggcttg agcagagaca acccagtgca 44880gttcagagag actgaagtct ggtgttccag gtctgagtcc tagctctagc tctctgtgta 44940actttgggat gtcccaaagt aacttttcac aacttgatag gtgttaactt gaattttgga 45000tacaggtgac tcttagccca tcccctctct gtgcttcaga tatgtcatca cttgggccat 45060atgacctctg gacacctttc ctactttcca caatttcaga gcagcagagc agactggagc 45120tcctgctgcc tctgagcttc agtgaattat cactcgttgg

agggaagctt caagcatttt 45180gttatctttc aagagcaaac acagtgtctg tcagcaagaa tatgtagcag atgctagtga 45240acagcagtga ttagggttga atgctggatt taaatatgga gcttaggctg tgaaggaagc 45300ctgaagaacc tagagcccca tgaagctgcc ctctgtgaga tctgacacca tttcccactg 45360atgagccatg ggtctaatgg gtagcactga ttccacatac agtatgtgag tgcaatacag 45420tgaaagcaaa gagaataaaa tgatggctaa catgatgttc caaactttaa acaggagaaa 45480aacacacaat tccattatgt ataagaaccc acacagagat caggagaata acctcattgg 45540agaatgaatg acctgtgtgg ggaatttagg gtagagttga gattgaaaaa atgggccgaa 45600gtcaggtggc caagggcctt aataccttgt atacaagatg tgtagtcaag gaagaccatg 45660ccttacttat gcatcaattc ccttgggcct acaaagagct gcctagcctg ggactgttgt 45720agagaaaagc tacagtgttc caatgacaca gggactcctc catgtatgta tgagtgccca 45780gctggctctg taataaatct tatttttatt tattaacttt tcttgcgcat tggcttgatg 45840catcagttgg aagtcagagg ccaaacgaag tgaacactga gccaaaggaa gttctcagcc 45900tttagggagg cagaattcac tttaaacaca ataaacaaat gaactcacat tatacaggag 45960agagtcagaa gatcccagtg gctggtgtca tcgggccata tttgcccgaa gtgcctattc 46020cttataggaa cccactccca gggttgatgg gctacatcct taggaggctt tatgcctatg 46080ttctcctgac cactggctcc tccagggctg gcctttttta gtctctctgt agaggttcct 46140gtagctggtt ggatataggc tttcacagaa gggtcagtgc cttgggtctg gttacaggac 46200tgttaatctt gctttgttaa gagtaatgtt atttccccat ttccaaattc tccagggaga 46260tggaatgtca aagatagtat gactgtagca cctaaatcct gggttccaga agcagagaag 46320agaatacact gaagctgtag aaaggcctat tagtccatgt cagagattaa ctggatgcga 46380ggaccatttc tgggatggtg tatacagaac tggagaactg gatagggagt cagaaacaaa 46440gagctgaaga tgatgccttt gaagtttcca acgtggataa ctaggtcaac agaatattac 46500tcaagaaagt attaacatag gattgagatg cagtcagtag taatggaatt gaaatttcaa 46560agtatatacc tcatggatct cagggggtgt tgagctgatc acctgggcta acactcctat 46620gaccctgggg aaaatcaaat gacctggtac tgtagccatg gtaggggtgt catcacctta 46680atccaactgg gacagtgctg tttgatattc atctggaact tggtgcagac ccacattttg 46740ctgggtttca ccacaaccaa ggcttttttg attcttttct cttttaacat cagtacatca 46800ctgcaaagtt aatcctcata taataggaga tgaaactaat tgcttataaa aacaagattt 46860ttacaacact aaaattgttc aagcatatgg gcatatttat agttgcaggc agtgtttcag 46920atgcagactg ttcttggctg cagtggttgt ttacaggcag catctgttct gattaaatat 46980ttgatgatta tccccgaatg ttttaaagca tagtactggg ctctgctgac tgtacaacaa 47040actggcattt ttgacctata gggcactggg ctaggagata cagttctgag ggaagtgaaa 47100gatagttaac aactgcacaa ctgacccttt attagttgca ataaagcaat ccaaccaccc 47160aacccactgt gatggctttc ctactattta aggttggtgg tgtcaaagag acaccctcct 47220gtacagtgtg cagtgaatca acatcatttc cacaaaacct ccttcctgca caaaggaatt 47280atcatacttt gttacgaagt aaaattttcc tgtatcagtc acaggagttc accagttaag 47340atactgttag ttgaagactt ctggggtgac ttaatgaaat agctcagcca tctggtttaa 47400aaactggatt cttctatccc tccacacagc tgtccatgca cctgcatcat ctcaaggctg 47460gtctccctgg tggtagaaag gctgacagta aaaactggag ccacatgatt ccttgcttag 47520gcagtgtttc ttcatactct cacatgagag caggcatgtt ctttccctag gctcacagta 47580aacactctgt caaatctcac aggcccaaag tgcttttggt tatccccatt ccaagccaat 47640ctgtggcatg gaagatagca ttaccctgac tgccttagac taatatacct actccacttc 47700tggggctggg aataattttg aggtcaacca tccaaactgc atgacagcca ttccatggaa 47760gaggtatggc ctaaatcttt ggggcaacct gaattcatga aaactctctt agatttatgt 47820aactattttt agaattcact tctgtatcat ttaattttac taataaaaat accaccttta 47880ccctaaatgt cagccaagcg taaggctccg ttgggacaga aggaactatc aaagctttgt 47940gtttttatac attagcagca tttgacaaag aaataactct gaaggaagga gaataaccag 48000gcagagtcta gatgcatgga aaagaagtct ttgagaaggc ttcgcaggct gaaggaaagg 48060gcaggactac ttcaggaata caacgtttaa gtaaaagagg ttggggtctg ttgatcttga 48120ggagagatga ggatggactg gaaaatagga gtgagatata gtaggagagg aaaggatatg 48180gatgatgtat atactgtatg ggtaaccatc agtcgaccct aagaagataa tagttgttaa 48240atggttagct atttaatgaa agaaacctga acaagtaata atcttgagtt gcaaagtggc 48300tggagtcaca caacagacta aatttctggt agaacaaatc cagcagctta tgtgaaggtt 48360accatgtctg aagctggata gaaaaggtct aacttccaac caaagtcaca gttcttgagc 48420tcggtacaca gagacagact gctaacagct gatgtgtcct cagcgagagt gtcctcattt 48480atatcctcgt tctcttctgc ctcctttttt tgttttaaac ttttgggaag tctcatcatt 48540caatacagtt ctaatatatc acaataacag aggcggccca atttctacaa attgactaat 48600tctatccctg aaaagttcat acaataaaac tatacaaagc atcattttca accatcctat 48660agaaaaattt ccctattaaa ttttaactct aaatcctcta tctctgttaa taaccttact 48720aaaaacttcc ccatcactgc ctgccaggga gatcaaaaga aaccaaattt aagaaacctc 48780caacacctgt acctgactga aaagcaaaca tacagacctt tcagtcctgc ccctactatt 48840cagctcctat cacagagaca tggtggatgt ccccttggga agcggatagc tcttaggtgg 48900aagctaggcc tgcaataagc aggccaggaa acatgtctcc caggccccat actcttggca 48960gaagcacttg gccaccagac agcatgtcct gtcaacctac agagttctta aaaacaaaca 49020ctgggaccca gaatagtacc ctgtggtcat agtgcccaca gttcactaag caccctcaca 49080ggtctttgac agaacactga ctgccaggtc acctggtggg cagagaaatg gaagattccc 49140aggcccaact agcatctcag ggaagaacca caagcagagc aactttcaga gctggtcggc 49200cagcgttggc acccagggaa gcaagtgcta ttccatattt ggagagaaca taaaactcaa 49260ggaaacagag aagtcctact caataccgtt ctcaactgaa aacaagagaa cttgcaaaaa 49320agaaacccag ttttctggag tccatggaga aatatgaagc caatgctcgg ctaacagagc 49380agaaagcctt ttataaataa tgccagtcaa agctccaaag gtcagagctg atgcatgccg 49440gattgttctg acaatttctt aggtttctag gcaacaagga gctggtcaaa cagctcacct 49500ccaaggacat actttataat accaccctgg tagacgagag cgaggcagca gtgaaggcaa 49560tagtcaagaa caggagaggg aggtaaagaa caagatgctc ttcagacagt ctggacaaag 49620acagtcccta cccagctctc aggagttgcc tccttaagaa ggtggaggcc caggcagctc 49680ccagggtgac cctaaagaca gactccaagg aaaagggtgt gagggcaatg gtgaatataa 49740ggaggtcccc tttcagtagg cagagcaagt cagttcaggt cttattttta gagtctctga 49800acagtgaaga gaagctttct gtggacagca ttccaccacc atgggagggg aaaggtgcca 49860tgagagactt ctccagtggg gcatacaagc attgtgcagt gatccccaag atccagccac 49920tggcaggaaa tcgaaaggca agcttcttaa atacatagta tatggagaca gaagtgtgga 49980gcactcccaa aatgaaaggc caagacccag gaacaacctc cacaacctgg agtatataca 50040aatgaaccca gccctgctga ctcagatcca caccatccta aagcaggggt ttctcatgaa 50100tgaaagggct aagtatttgg tggagagaat gagaagtctc ccactgccca cacatttttt 50160cttcagagat ttcttttcca agagcccctt gaataaagga agggagggag cactgaatgc 50220cccagaaatc agaatgcatg gtgcgggaag acgacaggaa atagttctca aagagatcag 50280aaaataaatt ggaagcaatt tcattaccac aaagaacaga cattttttct aaggcacccc 50340tcccctttcc acccaattgt cattcagcaa ctactgaata cttaccaatg aaaaacgtta 50400cccctgacct caaggctgct cctgagctct ggtagaaaat gctttccttg tctataaaac 50460atggcaagca aggcaggatt taacagtaag ggcacagtag cactgcaagc cttcaaatgg 50520aaacctggag acaagcagtg agaacaggaa gcaaaagcag ggcaggtgaa gctaggacca 50580gggcatctgg aactttccac acaggttgga tctccatgcc agacaacagt tttcaaggaa 50640aaatatctaa gaggaacatg actttgggaa actttttggc agtactgctt actgtatact 50700agagagtaaa agaatttggg gaacattcac caatttgctt cttcaggggc ttgggtaggg 50760aacgtgaaca ggaacctggc tctaatttct gaactttttt atcagtaaaa acaatccaac 50820aaacgaaagc tagtcagtga gagaactggg agggtctgcc ctccttccct gagtcaagcc 50880ttctgggggg acctcctgac atttaattaa gcaaagacaa cgcccactga aggaagctga 50940cctgaaagtg acacgctact gtgaaatgag catgaagtgg gagcttgtta catatatgaa 51000atggccagcg atcctgagca aagcgcttca gagcttgaga cctaagtctt ctcatctata 51060tactgagggc tggacaagat gatctgtcaa gccattttta tccctaatcc accaaaatcc 51120aatgctttag tttattgtca caaaagcagg tatcgaatgg ctatcctgca gtgcctccaa 51180tcaacattca gactttttcc ctgaggcaat ataagataac agttaacatg tttttatcaa 51240ttaggtggtc atgagataaa tatatatggg aagtggtagt ttttcactta aatgcatata 51300ataatggtac agctctcttt gaatagtatt tgtttatttc ttaaatattt aagttcctaa 51360agacggtaag aataacccaa ggaagtgaaa tcaatgtcac aaagcacatg gctaaataac 51420tgcaggtttg cagtgccatg tgtgagatca gatgacagaa gggagaacta cctttaggca 51480gaggcttctc atgtcccctg gagtggccat gtgctgttct acatgactac ttccacttcg 51540gttatgtaga agctatttaa agcacacaga tgtttgtgat gagaaaaaag ccacccttaa 51600ttgaataatg gaaattataa gcatgatttg agggtggggg tggaggtggg agtagagatg 51660ggtagaaagg agtgcaatgg aaacaaagga gccttcataa aattcaagtc acttcttagg 51720ataacgtgat tgatttactc accaccttct taggaacata aagcaaacaa gtgggttttc 51780cttttactgc ttttctgaaa tgagctacac tcaagaaagc agcacggggg ttgtgctgtc 51840cctgcacagt ggcaggagag tatgaggagc aggtgaatgc cacaacagct ccatccaaga 51900tcatttttca catgcaggaa ccattcttat actacccttt actggtaatt tctgtagaaa 51960tctggaagtc tggttgacac cctcctgtac agtgtgcagt gaatcaacat catttccctt 52020ggactttgca atcacggtgg cattcataca ttcattcaac aagtatgtat gtacaggaca 52080gtggagtaaa gaaaacagat agttcttact ctcacgaggc ttaaaatttc aggagggaac 52140taggcagtca tgaagtaaac ataaaaacac agattgtaat aggcactaga gaataatgaa 52200gactttgggg aacacaattt aaattggaaa aatatctcag ttccgttaac acatgttgaa 52260ggggaatagc attgttttgc tggatttggg gcctggatgc aagcatgttg ggtatgcatt 52320gtagggtaat gcatttcctt ccatttgggc ccaagtgtat atttaccacc cagttgtgat 52380gagctgggat cctcctgctc aatctcagct tgaagcactt ggaggttatc tgcctgctgt 52440gggtgattat tttggagcaa ggtacttcat ttgcctcaag aaacagattt gataccacta 52500ctgtgccctt ttggaacaga gaagtaggca agaccccagt gtgaggcaga gtgatgggat 52560ctttagggac ataattgatg atgtaactga tgatgatttt ggagtttata catttccaaa 52620gtttcaaaat attttcactt ggttgattta tctttatggt gatgacacta cgtagatata 52680tgcccttctt aaaagttaca gtaagaggct gggagcggtg gctcacgcct ataatcccag 52740cactttggaa ggccgaggtg ggcagatcat gaggtcagga gattgagacc attctggcta 52800acacggtgaa actccgtctc tactaaaaat acaaaaaaat tagccgggcg tggtggcggg 52860cgcctgtagt cccagctact caggaagctg aggcaggaga atggcgtgaa cccgggaggc 52920ggagcttgca gtgagctgag attccgccac tgcactccag ccagggcaat gagcgagact 52980ccgtctcaaa aaaaaaaaaa aaaaaaaaaa aaaagttaca gtgagagttg acattgagaa 53040aagggaggcc cagccagggt tatgcaaaga cacagtaggg aggaggatgg gaagtttctg 53100agacacccca gtaactgcat gggtccacaa agcacatgta cagtctgctc tatgcactgc 53160aggtacagcc acgaataaga caaagtctct gccctcatgg agcttggtgt ctgtctactg 53220gagcagacaa aaacaaacct gctttttctc atttgcctct actgtgggtt ccgtgtataa 53280ctctcaaatg ctagcatttc catgcattcc atccttgtcc ttctctcctc tctgcctttt 53340ccaggagaat tttcctagcc acaggttcag ctatggtcta tgtgctggag tcatacattt 53400ttatcttctg tgtacgtttt tctctagacc catattttta attgccttag gacagctctc 53460cctggatatc cctcagacac aactaaaaca tcattcaatt gtactattaa taattttccc 53520ttcaaaatcc actcctcttc tctatagacc taacagcaac actgtccgtc cagctctcaa 53580aacctgaaat gtgggagttg tctttgactc ttatctttcc catgcatgag cactgaatca 53640atcagagtcc ctagcatgtc ttggactgtg gcctcccttc tatcttcaga ttatctgcct 53700ttttagaatt atggcaataa cttaactgca aagaccacat gtgcacttgt gctctttcag 53760tttgtaataa atataaaatg aaaagtgatt atgtctttgc ttaacgtctg caaaataaaa 53820gtccaaagtc cttggtatgg tctataaggc cctcattatc tgacctgcct gccttcccaa 53880tctcatctta atcccttaca gcctgacact cagacatact agactttcca cctaactcac 53940tcacatacac ctatcctgta tgtcaagatt cggcttgacc ttcatcacct acctatctgc 54000agtatttttg gatctgaatt ctctgcccac attgtacttt cacatacttc ttttaccctt 54060agtggtttgt acttatgcct ggtctaacta gattgtctcc ctgtaacaga cttcttgatt 54120caacaaagca gctctgaatc agcctgaaac ctatggcaca ctgcaaaatg gcaaacattc 54180aataggtatt tgcccagtaa atgttaatga aaggaaaaaa attcaaactt cagttggaat 54240aggattagag acaagttaaa aaaaagtttc ccatagaaat cttcctcatc gtaaatatca 54300tcatcctaaa tgtcccgagt ctttccatgg gtctaattta ccaaatcatg aagactctct 54360ttctcactgt gtatgtgtag tgggagaggc agagacagaa cggttttttt ttttttttgc 54420aagtctgctc tctggattcg tttttctggg gatgaaatct caggctatgt agcttttctg 54480gtcccttttt ggtaatcaac aaatcatcag tttctctagg tattaaaaag cctacacttt 54540tgaaacacca agaggccaaa ctctctctta attgaaaaca taattctgcc tcttactgag 54600gtctttgggt gggaagctta aagacagcag tgttggggca attggttttt cttttcctcc 54660ctccaccttc tttcccattc aagagctgtt gctgcccttt ctggagggga gggaattaga 54720aaaaaagatc ctgccttacc cagtcactgt taattattac tttgagccag ggtggggaat 54780gactttcttt cctggaatac accctgctgg aaaccacagc tgagattctc taagctggca 54840gcttccaacc tctcctccct caacagcttc aacattatat ggcactcagc tgcagggtgc 54900ctggctccag gccggcagtc catttgtgca gggtagtttt caagatggct catagcccat 54960ctctttcagt gaccagcgtg gcctatggga aacatacatc ttgactccat tattggtagg 55020agtactcttt agttaactgc cacaggccag ttcagacacg tcttaccctg agagcctttt 55080cagagtcagg taccatccct gtgtccccca gcatctgaag atcacaggtg aaggtctcca 55140agtagttcac ttaaaaaata cctcagtagg tttaagaaca gggaaagctc ccaattcatt 55200ctgtcaggag tatgactctc atccccaaac tggacagata tataatatga aaactacaga 55260ccaatattcc ttatgaatat agatgcaaac attctaaaca aaatactagc aaaccaaatc 55320cagcagcata gaacaaggtt tatgtagcat ggacaactga gattatccca ggaatgcaaa 55380gttaattcaa tatacaaaag tccattaata caacatatta ataaaggaca aaacaacatg 55440aaaatcttga tacagaagaa gcatttaaca aaacccacag cccctccttt tttttttttt 55500gattaaaaaa cactcagtaa actaggttta ataaaagaat ttcctcaact tgaagcaaac 55560ccacagctag ctaacatcat actcaatggt gaaagactga atgctttccc cttaaaatca 55620ggaacaagaa aaagatgtct gctcttgtca cttctatcca atattgtact ggaggtgcta 55680gccagcacac taaggcaaga aaatgaaata ataggaatct agactggaaa ggaagaagta 55740aaagtatttc tgttcacaga tgcatgatct catatgcaaa aaatactaag tgacaaaaaa 55800agaattttag aaaagctaca atatacaaaa tcaatataca aaaattaatt ttatttctaa 55860caccagtgat gaatacaaaa ataaaaatta gaaaataatc atataattaa agtggcatca 55920gtaaaatact tagaaaaaaa ttaaagacgt caagacttgc acactgaaat ctctaaaaca 55980tcactgaaat aaagacctaa gcaaatgcaa agacatccca cagtcatgga acagaaaacc 56040taacaccatt aaaatagcag ttatttctca aatttatcta ccaattcaac taaatcccta 56100tcaagatccc agctgttttg caggaattga cacaatgatc atataaaaat tcatatgcaa 56160tgcaagggac ccaaaacaga caaaatgatt ttggggaaaa aaaaaaaaaa aatggaggac 56220ttgcacatcc caaatctaaa cttactacca agctacagcc atcaagacag tgcggtgctg 56280gtataacgac agacatatag ggcaatggag taagactgag aatccagaaa gtcttatatt 56340tatggtcaac tgttctttga caagggtacc aggaccattc atggggaaat aatagtcttt 56400tcaacaactg gtgcttgggc agatggatat acagatacca atgcacttat gcaaaaaatg 56460gatgaaatag gaaacttcac tacattctac tgcatgctcg gtcattttca atcatttagg 56520tggcaacact gacaagataa cagaaagatg gaggtaataa catgtgaaag gcaaaatggt 56580ttgttttttt ttttaaaaat gacagtctct atcatgaatt tacttacact ccaggcaaag 56640gttattagaa gaaaaaaaga tgtaagaaaa ttccttaact gaaatgtgga aagagtatca 56700agaggagacc ctaagacact ctgtaagaat cccagtgact cctcactgtt caactaagaa 56760atgtacccca ttatgctgtg ctaccacgga agcattggag gcactttggg ggttgatgaa 56820gtcttcatgg atgaggtact ttaaatatct ggtcatgaag agtatttgag aaatatgaca 56880agtgaagatg ctgggtagga atgcagcgag gaaagtgtgt aacacaaggc caaattggaa 56940aggtcagtga gggggcaatc tgtggaggca ctgaatgcag aggatattaa aattagcaag 57000atagtgttct agcacaatga aaaggattgg aagagggaga tgagagtcag ggagtgaaat 57060taggcagctg ctaagaatgt ccgggtgagt cagaggatca ggacctgtaa gggcagtata 57120aagaaggaag gaatgatggg agaggtttag ggggaaagaa gtgacaacct gtgacaattg 57180aggaatgaga gattttgatg attggggtga aggttacatt tcttaaaaag aaacaaagaa 57240tggtcggtcg atagagatgc aagatgaaga attttgtttt taggactact gactatgagg 57300taacaaagaa atcccagaga cagaggtctg agcaaaagat atgggattgg ggaagaatct 57360ttgggagtga ctgaggttga ctagagggaa ctgggcaaga acaggtaagg actttaagca 57420gaattggagg agtatccatc taaaatctgg gtaaactggg atggaaaaac aagtagccag 57480agaagcaaca gcccaagcta gggtgttgtc aaggttatag atgttactga ttttggcaat 57540gaggagatta taaggatcct tgaagactgt actttcggtg aaactaccat gcattagtag 57600cctttcacag tgatatctac cccacacctg catcaaaatc ttgcagtgcc tattaataaa 57660tgccaaccca ctaacgggga ggggagaaaa gacttgggac tctgtacttg taaaaccctc 57720taccctcccc aggcaattct ttacacacta acactgttga ggtaaaagga gacaagtgag 57780ggagcaaatg gaaggtgtgt ttttggatta tacagtggct catggaaggg tgggaggggt 57840acagatggcc cttagacact ggcggaaagt cagagaaaaa aaattacagt aagcaggtaa 57900agggatcaag actacacagg gactagtctt gggacgacat ttcttcctct gacagtttgt 57960atggaattct tgagaaaaat tcctcgaagg ggcctgaaat ctcagaatgg tcatgttttt 58020aatgggaata gggagtgatg ctgccccatt acaaccatct gttctaacag aatgtctgta 58080ccgaggaggg atgagtaaca tcggcaagtt ctgttcgaag cctttttcaa gtttcttttt 58140gatatgtatc tatctatcta tcatctccct atacaagcaa gcatccccaa aagtagttgt 58200ctcaggaaac cagggttagg atgaccagct catttgctgg agctgccaag gtccagaaaa 58260gtttggctgc aggctgtttt catgttttat gtatgtttga aatgttctat aataataaaa 58320ggttaaaaaa gtttacattt atttggaaaa ccagttactt tagtttatgg ttcctttttt 58380tccctccaga gcttcctgga gattgaggtc taattcaaag aaaaccaaaa tatataatag 58440agtacctggg caaaaaaagt acttttataa cataacattt ggggtagagg aagtatccac 58500tgtagtcaaa atgtctatgt tttgctcttc cttattgttc agggacattc cattaaatag 58560taatgaaaag gcagcaaaag taagaggagt gacaacatgc ccggcataat taagcaagct 58620agagcagcta ttctgtgcaa ccgacgattt tttttctcta aaattttaag ggtaggttca 58680ttctgactct gttaaaagtc tacttgatgt gaacaactct atatctgata acctatttca 58740attaccactt taaaacttgt catatggata cgttattaca attgtagaac tttaataaat 58800accataataa taaaacttga gaactgaaga gcacacattt cttcacgaat ttattatata 58860aaacgccctc agagtattta atttctcctc actttaatta cacattaaga agcacagtgg 58920atgagaagcc tttaagatga ctacagttgc acgaaggtcc ctttcatcaa ggtagcgtat 58980gtaccctaac agtgttctaa aggctggccc agaaaaaccc catgttacct tatcacaata 59040tggaaagcat tgtcttcttt ttccactaaa ttaaattatg gtgaaaagtg ccacagtttt 59100atttagcatt atggtacata acaaacagtt ctgtctcaat tatgaaaaaa attaattaaa 59160ataatcctga aagacatcct ttttctcccc ccaatgattt gaaagctgca tttttcctgc 59220caatttcaaa caaacaaatc atcaggttga tctacagtaa tcagttaaaa caatcagtca 59280atcaatcaat caatcaccaa ggcacaagct cagcacatta gctatagctt gtagcaaaag 59340gatatatcaa tgtctcacct tagttaaaaa tacataatcc ttttatttta taatgcaata 59400aaagaaatta acaacatcac atacacagaa gactaggaaa ggggaaacta cttacttctg 59460gaaatcagta atgtaaacct acttgtactt ttccatagta catgaaagta acgtttaaca 59520tgttttgaat taattaatta aatttaatct gtggggctat acaatgtaat tcttaggagt 59580aatagtttca ttcatttcca ggtcagctta ctgtatgatt aagtaacaca aggcacagta 59640gccatctttt tcattatgtt gcaacactga tcacgtgcct cgataaaatg gctgattcaa 59700caagatgatg gcaacacgaa ggggagactt tggattgtct atttaaaatc taggtaataa 59760gtaagtaatt aataaaaact ctatcttaag tgcactttca catgcttttt gtttataata 59820aacaaacaac aaacttccta actttgttgc aataggcttg actaccattt catttggcca 59880aatgcacttt ccccagtaaa cttaaaacaa caacgagaac aacaagaaca aaaatccctg 59940tcctttcata tactaagaaa gaggattggc tactgaaaca gttcattgca agacacatga 60000agacgacata ctgtggcatg agttgttttt gtttttaatt tgttgtgctg ttactaaagt 60060tctgagggct gcagttaaaa cattccaatt tctcccttcc ttccatcttt ctttattgat 60120tgattctcaa gattttgcac agaaaactct ttgggggcta gaacagcagt aattgcatca 60180cactgttttc aagacttcaa gtttcaaaag caaatcatta

aaaaaaatac agttcctgat 60240ttgagttaga tacagggaca aaaaagtagc acatacttga aggttacgtg gtctacaaat 60300ggtggcaata ttttccttgg gagagtagtt ctgttggtat atatttttta aatactcaaa 60360aggctcaacc tcaagcagta ataaacacaa gcaaaagtga tttaaccctt aaaataaata 60420ttcagaaaaa cctctctgta catacaagtg aaagaatatg taacactttc acgcaaaaaa 60480ataattataa taataataaa ggatttgttc atatatgtag ctgaaatctg ctgttccagc 60540ccacatgtcc ccaataaaga agggaggcac agacataggt gactactgtg gttgactatc 60600ttacagcctt tttgtactgg gacactatca ccaccaaaaa tttatccctc gttatatttt 60660taaaattttt taaatttttt cttttttttt ccttcctttt ttttgtttta ttttgttttg 60720ttttgtttta cagcatgcca aatcctttgg catacgtgat ggccttcaac aatctctctt 60780taagtttttc tttgcttgag tattccggaa gtaaaagcac attaaagcaa gtatgagatg 60840taggtaacct aaatagagaa aaggggaaaa aaacaggaaa actgtaagtc atgggaaata 60900cacttagaat taaatgctcc tatttttaga ttgtatatag ttgagacggt ctgcaatgca 60960aactatacat taatgcaaat cataaacttt ttgttgtgta actaccaagt tgcctttatc 61020ctataaatta ctcaaagcta gtgacgatga taagatactg tatccattga gtttttacta 61080cataacagat accattttag gtactgaatt cttacagttc atttaactaa atctttccac 61140aacaaaacca cagagaggac atcagtaaat gcactttaga ggttaggtca ctgagaagtc 61200aagtaacttc ctctaaggtg gagaaaatac tcaaacctgt tttacaagac tgcaaagtgt 61260gtgctcttaa atgcttatta gaaacactgc tggcaatatg actaagaaaa tgatttgata 61320acaggattct agcacaatca aatgataatc ttccgagcct caatgtaacc attctaaata 61380gatgatcatg ttatatggct ttcaattaac aagctgggaa tcaaaaaagt aaatgaatca 61440cactaatttg attccaaacc aatgtgagcc ccataataat ttttaactag ggcaatttct 61500taaaagtttc ctcacacaat gacagcaaag tattttctca attgtctaat atgatttggg 61560gatttgtata taaaatcacg aatgtgctca gaaactataa agacagttca tatgtatgtg 61620acgaggaatg caaggttttc ggtaggtata cagtcacaag ttaataatta cctacctttc 61680tgtgtctggg ccatttttgg ctataatcat ctttaatttt cctagtcctc ccacaggtgc 61740tctgtctgtg cccgttgtaa actgcaagaa gagtcttttc tgttcatctg taaatgaatg 61800aacgatttcc cagaactccc taatgagaaa aaatacaata ctggtttcag tttggcattc 61860attatgactg gtactaacat aagcttatga tttgcattaa aactatatta agagacaact 61920tggaagttaa ttatcaggat agtatcactt ctggtgattt aaaaatttcc aagcaaattt 61980atcctgaaca gctctgaata cgtaaaaatt tagattagat tacaatatag taaaatatta 62040gtactacaat agtaaaaaat tgagaaaacc caagtgtgta gtaacaggaa gtgactattt 62100aaactatggt ataatcacat tatgcagtca gtaatgagca aaagacaaaa tcctatgaat 62160tgaaaaagac tgaaatgaat atttggaaaa attaactcca ggtgcctttg ggtatatatt 62220ttatttatcc tttctccttt attctcctgg tctactcatc ctttaactca actttgggag 62280gaaaaagtga tacagattaa ggacaaaaga aaaaaagacc ccctgcccca accaactggc 62340cccaaatgca aacaactaca tacctaatag cagtaataca aaagttcaat ttttatatga 62400actagaatac tttaaacaag taatatgtgc tgtgtatgga ggaggagaat tattctgaca 62460tttctgccac ctgcagttat tttaagagaa tgatttatcc tgtggcattc ctaaaatcta 62520tgtaataaaa gctatgtttt aatgacactt atgttagttt gagttctaaa aaacgaaata 62580caagctcata aagtgcaatc ttgaagttta tttgaataat caggcatcta taaaacatat 62640atacactagt tcatagctaa ataaattttt ttttttttga gacagagtct tgctctgtcg 62700ctcaggctgg agtgcagtgg cgcgatctcg gctcattgca agctccgcct cctgggttcg 62760cgccattgtc ctgcctcagc ctcccaagtg gctgggacta taggtgcctg ccaccacgcc 62820tggctaattt tctgtacgtt taagtagagg caggatttca ccatgttagc caggatgggt 62880ctcgatctcc tgacctcgtg atccgcccac cttggcctcc caaagtgctg ggattgcagg 62940catgagccac cgcgcctggc ctatagctaa ataatgttaa gattaaaaaa ttaaaaaaaa 63000aattaaaagt atttttagtt gcttatataa tataaaatgc attttaatag ttatttagaa 63060agttctttcc agagccaggt gtggtggctc atgcctgtaa tcccagcact ttgggaggct 63120gaggccagtg gatcacctga ggtcaggggt tcaagatcag cctgaccaac gtggtgaaac 63180cctgtctcta ctaaaataca aaaattagcc gggcgtggtg gcagatgcct gtaatctcag 63240ctacttggga ggccaaggca gaagaattgc ttgaacatgg gaggcggagg ctgcaatgag 63300ctgagatcac accattgcac tctagcctgg gaaacatttt gagccatctc aaaaaaaaaa 63360aaaaatttca tctcaaaaaa aaaaaaaaaa aaaattccag aatgttagca gagaacatca 63420cacatcccaa accagtaatt acaattttct accttttgat attctaatat cccaacatac 63480tatttttatt accaaaatgc tggcattttt ggtgctgcaa caccatttat ctgaaataac 63540ttaaaagttt tattagaatt cttggctttc tccaatctct tgccacttcc cttccctgcc 63600cctttacaaa tccaccagtc agcaagagaa aacaaaaaag aaacacacaa caacagaaaa 63660ctggacatga aacttgcagg agcatctctc aggtaaggag gagaggaatc ggaccccagt 63720tcatgatact ccttttgggg aggctgacaa gagaaaacaa gtctgctgta gcacagaacc 63780ctccaacata gcagaaaagc tgccctgcag tgggtggtaa taaagcctcc tcacactgca 63840ggagggctct gaagcttaac tgaaccagct acacctaagg aggtagacca aagagcacca 63900ccgaagaaca gagacagacc ctgcaggaag tggcagatca gcagtggtca caatgaccag 63960tctggctata ttattacgga gctgtttctt tggactttta aacgaaacct ttaaaaattt 64020tatacaatga aaacagggaa caaaatgcat ctctattcct ataagtgtta tgtgtgttac 64080attaacattt tgaattaaac aagaatgcat atatttagaa agctgaagaa aacacgaaga 64140agcttccagg tttccacata aaagtggtgg ctgtatcgat cacatcctac tcacatctct 64200aaaaatctac ccggatcaaa aaggggaaaa taaaaaaaaa acctatgact taggtctaga 64260gtaaaactag gagacagaac aataaactcc aaataccaaa gaagtgggga aatgagcagg 64320gtccagcagg agctacatcg aagagtggct tgtgcggaat catactgatt agggatcacc 64380caaggccaga ccccaccccc tccaccacct gcccctcaac agagcactac tgagtgtagg 64440ttagagcact ggcaacaggg tggttaaagg aaggactaca gaaaagtact tgggtccagc 64500aatggcaggc tcaggaaggc accatgcatg aaaggaaggg gggtaccttc aaaagcagaa 64560gtgtccctta agcggctgta aaggggtgga agcagctaat gaaaaagagt cttcattaag 64620ctacatggag ctgaaaaatc agaaagtggt gattctgccc ttactaaagc aacagaagag 64680ggatcccttc aaccaagacc cctaaagcca cacaattcct ccctgctcca ccatgaggtc 64740tagtaataac aggtccagaa aaaagtaaca tctgttatag aaacataaac aagaaaaaca 64800gaattcggaa gtctaaataa agttattatg ggaagagtct ggcgaatgag aagcaaaact 64860ttccggtaga caaaagtaga ccagaaaaat tcagtcataa acaatgggaa actagtaaca 64920ccacattcca acacaaaagg agaatgcagc agcagacaag acagaccacc agtgatgaga 64980aatacaaatt cagaaagaaa tgagcacatc ttaaataaaa ataagaatac taaaaaatac 65040tgaagaaata tgcaaggtaa ctttggaaat aaaggctaat tttattataa ggtgaccaaa 65100gaagaagcag tatgactaaa atttctactg aggacagact ctagaaaatt aaaaaacaaa 65160gaaaatgaat aaaataaaca taactaaaga aatgcatatc aaagcataat aacgaaaaag 65220attaatagct aactacataa acataaaacc tttcatttgg caaaaaaatc ctataagcaa 65280ggttaaaaga caaatgacaa actgggaaaa aatatttgcg aatttaccag cgttaaagga 65340ctaatctcct taacatataa agtttctaaa agtgaagaaa acgaccagct gagcagcaaa 65400atgagcaaaa gactatataa aactataaac agctctacaa gaaaaaatgt ccattatcat 65460tcataattga ggtaagctca aaaattgtaa ctatagtaaa ataccatttc tcaattggca 65520aataagtaaa catccacatt taacaacaca ttctgtttgg aaagttttaa gaaaagagat 65580actgttgtaa actgctggtg ggaatgcaaa atgttatttt tctgtgaagg aggatttggc 65640agtatctagc aaaattacac atgcatctac catttgatgc aacaatccca cttctaacaa 65700tttatcttaa tgatacttta catatgtacg gaataatgca tgaacattaa gaggattcac 65760tgtgacatta gtactaacag caaaaggtta aaaataacct cgatgcccat aaatattgat 65820aagctatgct gcatccacac aaaggagcag tattcagtca tacaaaacaa aatggaaaac 65880acctctgcaa taatgtggga tgattctcag gatacactag gaagattatt ttcctagttc 65940tgtcctctga taaggcctac aagcagtaac gttcaaaagc aaaggccata ctttgcatct 66000aaaatctgac ttctaaatgc cattctccaa caatttattg gaaaaataac ttattccagg 66060cctgagaatg aatgttcgag attagttaga aatctcaaaa tctaataggg tcattttaaa 66120agcacacgat agctaagcaa tttgaatatc atttagagta atgactgtga aacgtattaa 66180atacaaaaac attcattagt tcgtaatatt caaaaaggca gcaaaaacca accaaaaaac 66240aaaattaaat tgtcactatt aaaattatta cattaactcc ttactctaaa aaccttaaat 66300ctattttatc atgccttttc tgtagaactg tttttcaagg taatcaaatg gcacccaatg 66360atgagaaaaa agaatgccag gtatatatgt aggacaagca gatggaaatt ttttttcccc 66420aaacagccat tttgcaatcc ccaatgagat aacagattaa ggtaatgata ctgataaata 66480cgaaaatcag gtgaagggca ggtagcaggt tctggaaaga tgatgatggc aacgacatag 66540ttattattat tattattttt tgttttattc cctcccaacc tcccctacaa aaacagcaaa 66600ctgaatgaga aaaccaaaga cccagagaca ttatctacaa caaaaccatg aaccattgca 66660tataattgga cagaaacaaa gctctgacaa ctacaagact ggttagtaag gaagcagatg 66720caaggtaact gactgggttt ctgacagccc tgagaacact gccaacccac tggaaagcac 66780aggccaatct gaaaacaggg cttaaagttt taaaaagttc tacaggatct aatttgcaga 66840tgattacaaa gggatcatga tgtagtaagc tctgggccct taaaacaccc gaataccaaa 66900cccccaccag aaacaaacct tgtaccgagg aaaaacttct gggaataact tccgaatgga 66960ccaggtcagt aataaaaacc aaaaaaaagt tcaaatatat gtgtgggata gaggagtaaa 67020gaaggcagtt tcagaaagca caagggcata tttttgacca ttttacaaaa acacagactt 67080ctcctcgtcc ctaaaaagcg aaaaaagtta tcctggccca tctctccctc gtccaagtat 67140gagaaactca tttcactcac acacacacac acacacacac aaataaataa ataacagaac 67200agagtgaaac agtgtaatta ttagagaaaa agtatgtgca tatacatgag aatggtgtcc 67260ctacagaaaa tcaaagtaca tgaaacaata tgcaaataaa acatgaaaac tgtaaacaaa 67320tttcaaactg agctaaaaag aatttaaaaa ataacaaatc attagaaatg ggaaatttct 67380gaatgagggt aactgaaaaa aaggaagaca tgacacaact aaggagtaat tagaaatgca 67440aggaaaaaaa tcccatcaaa tacaaagaaa ctaaaactaa actagaagaa acataaaggt 67500gaataaaaca gaacaataat accagaacct gaaggcagaa gaaaaatctt aaaatcaaaa 67560agaaacaaac ccatgcttga gactctcctt gctggtccag agccacagtg ctgctgtgta 67620ctggcaggag gaattctgct ataattggtc ctggcagtgt tcacctttcc tgcaagtgtt 67680ccacagccca gggacacaat gtggtcagga gcactgccag gaacaccagc aagggggagc 67740ctgccacaac aggcacaagg ctcaggaagc actctccagc ccacgaaaat ctagtggggg 67800tcctcttccc tcacccaaac acactctgcg cagctcagcc tggagaaggc ccctcaagca 67860gcactactag gaaccagtgc gagccccagt ggactgagat aaaagcaaca aaccaaaaca 67920gcaccataaa gcctctgaaa aatggtcact ggaaccacag ctcacaaata tatgctagta 67980cctacatgct ataccttaac aggttactac tgaagttaaa atttttaaaa agtacagagt 68040ctcctgatat aataactaaa atgtccgaga cacaatccaa aaattatctg tcataccaag 68100aaccaggaaa attacatgaa ggaaagaaaa cgccaacact gggatgagta agatgctgaa 68160attatctggc aaggatctta atgctgccat cagagaaatg atttgacaag caattacaag 68220ttctactgtt tcaaaaacga aacaatagaa aatctcagca aggaaacaag atacaaggaa 68280aaaaaagcaa atggaaatta tagacctaaa aaatacaata ggagaaaaat aatcattaga 68340tgggctcaac agtagagtga agatgacaaa ggagagaaat ctgaacaaaa gagagaaaat 68400agactgaaaa atgaaccatg cctcaggtcc atgtggaaca gtaacaaatg actcaacatt 68460tgtatcatta gaggcctaga atggagaaaa gagtgacgct gaaagaacac ctgaagaaat 68520aatggttgaa aattttccat gtttggcaaa agacacaaac ttacagcttc aagaagctga 68580gcgaacccca aatagcacaa ataaaactat tctttgggaa taaagggaaa ataaagtcat 68640cttcagaaag agaaaaacaa aaagaatttc tcactagcag aactgccttt gaagactggc 68700taatggaagt aataaaagat ggaatcttgg aaaaaataat ggaaatagca gaaacatggg 68760tacatacaac agagcatcac tcccttgata agttctataa gtcatattgg ataactgata 68820caaaaactgt aatagcatta atcctcaaaa caatactatt taaaagtggg gaaagagacc 68880caactgaaat taaggtttcc atatctcatt ccatgtaatg gtattgatgg cagtgaatgg 68940tggtaagtca cattatctat aatgtgatac ccacaatgac cgtgaaaaca actataaaaa 69000acacatagaa ggccaggcat ggtggctcat gcctgtaatt ccagcacttt gggaggctga 69060ggtggacaga tcacaaggtc aagagttcga gaccagcctg gccaatatgg tgaaacctca 69120tctctaataa aaatacaaaa attagccagg tgtggtggca tgtgcctgta gtcccagcta 69180cccaggaggc tgaggcggga ggattgcttg aacctgggaa gccgatgttg cagtgagctg 69240agattgtgcc actgcactcc agcctgggca acagagcgag actgtgtctc gaaacaaaaa 69300caaacaaaca aacaaaacaa aacccaaaca ctaaataaat caagggtctt aaaaatctta 69360aaaaatgttc aagtaatcat ggaaggcaat aaataaataa ccataaacag aagacagaaa 69420cagaaaacaa ataataaaat ggcagacctc tccctaacat atcaataatt accttaaata 69480taaatggtct aaataaaaaa attaaaaggc agagactaac agtttttatt aaaatgacaa 69540ctatatgctg tttacaagag attcgtttca aattcagcaa cacaggatga aagtaaaatg 69600atagtaaaag acagatcatg caaacattaa tttaaaaaaa aacaaatggt tacactcata 69660tcagattcag agcaaagaaa atcgcaaaac agaaaaaact tatataatga taaaggacca 69720atcaccaaga catagtaatt ctaaatgggt atgaaccaaa tgatagagcc tcaaaataaa 69780tgaaacaaaa cctgataaac agataaatct cagaagcaga cctatataaa tattctcaag 69840tgatttttaa cgtatgtgaa aaaaacaatt caatgaagga agaacatacc tagaaaatac 69900ataaggaacc cttaaaactc aacagtaaaa aaccaaatgg actcttggtt tttaatccca 69960gagaaataca aactttttgt ttacaagtaa catcacagtt aatggtaaat attatagtct 70020ctctcaataa aatgaggaat aaagtaagaa taccttctac caaaggttct agccagtgca 70080atagaggaac agtaataaat aacataaaca ttggaaagaa agaaacctgt cttgatttgt 70140agatgacata gctgtttact agaaaaccct atgaaatcta ctaaataact actagaataa 70200gtcaatttag aaaggttata ggatataaga tcaatatata aaaatcgatt ttatttcaag 70260atagtaataa acaagtgaaa agcagtattt aaaaaatacc actaatagca ttagaaacat 70320attttaagtt tattcctaac caaagagtac atgatttcaa cattaaaaac tataaaacag 70380tgctgagata aactaacaaa catctaaata aatggagaga cgtacggtgt tcaaggattg 70440gaagaatata ttgttaaaat gtgaattctc tccaaattga cctgccccag tcaaggcaac 70500ccaaatcaaa atctctgcag gctttgccct tttgtttgta aaaattaact gattctaata 70560tttatatgca aataaaagtc aaagcaacct aatgaaaaag ttgtgggact cacagcatct 70620gatttgaagc tgcagtaacc ataacagtgt cattttggca aaaggacaaa acaaattaat 70680agaacagaat ggagaatcca gaaacacggc tcacacacag attgattttc aacaaaggtg 70740caaaggcaat tcaatgggga aagaaaagtc tctttaacaa atggtactgg aacaaataaa 70800taaaactatt tagaaaatga atgacatcca ctgtctaaca gcatcacaaa attttattgg 70860tggtggatca cagatttaca cataaaagtt aaacctatat tacacaatta gaagaaaaca 70920taagatattt gtggctttgg gctaggcaaa gatataaaag gacataaaaa accataaaag 70980aaaaaataaa ttagacctca aaaaatgaat aatttctgct caccagaaga cacttaagaa 71040aatgaaaact tggagaaaat aatcataaac tacttatcag aaatgggcct tttatataga 71100atatacagta ttccccactt acccatgggg aatacgttcc aagacctcca gtgatgtctg 71160aaattgcaaa tagtactgaa ccctgtatat actatgcatg atttattttt ccttcttcac 71220aatttcatag atacaatact catttttacc atagatttta gcaacctcaa catatgattt 71280ttaaattctt tccttaaagg aagcactttc cagcttctct ttggcatata tattcgaatt 71340tccaacatca ctgctcttgt gctttagggc cattattaag taaaataagg gttacttgaa 71400cacaagcatg tgatactgca acagtcgatc tgataaccag tagactaaaa gtggtgggta 71460gtctagacaa caacgatata ctggaaaaag ggataattca tgccctgggt ggaaaggtgg 71520gagatttcac catgttactc agaatagcat gtaatttaaa acttaagagt tgttggctgg 71580gtgcagtggc tcacgcctgt aatcccagca ctttggaagg ctgaagtgga agaattgcta 71640gagtccaggg gtttgaaacc agcctgggca acatagtgag accctgtttc tacaaaaaaa 71700taaccaacta aaaattagct gcacacctgt agtaccagct actcagaggg ctgacgtaag 71760aagattgcat gagcccagat actcagagga ctgaggtaag aagattgcat gagcccagga 71820gtttcagttt ataacaatct gtagtcatgc tactgcactc cagcctggat gacagagact 71880ttgtctcttt aaaaaaaaaa aaaccaacga accaaccaac caactctccc tgccctgcac 71940ccgccaacac acacatacat caaaaaccct gaattattca tggaattttc tacttaataa 72000atatttttgg accactgctg accaaagata actgaaacca aaaaaaagca aaactaagga 72060taagacatac tgctatattt taagaaaata tctatagtca atagaaatac aaaaatccaa 72120tattaaaaat gaggaaaaga cacttttagt taaatatgca cctgctatat gctttggaaa 72180ctccactcct acatgtttgc ccaggagaaa taaaacattt ccacaataag actacacagc 72240tttattcaca atagtcccaa actggaaaca atccaactgt ccaccaacag gtaaaaaaaa 72300aacactttgc gtattcatat aatgcaacac tactctataa ttttttaaaa ggcactataa 72360atcaatgcaa taacatggat gaatctaaag agtcttcatg cagtacaagt acataagatt 72420ccattcataa gaaattctag agcagacaaa agtaagctac agtgacagta attaggacag 72480ttattgtctg ttctaatggg ggatggagat tcactggaag ggaaaacatg ggaactctgt 72540gctgatggac atagtctaca ccttgactga aatatggtta cataaatgta atttttaaaa 72600actaacccaa ttaagccctg ggtatttcac tgtattcaaa ttttacccca cacaacatcg 72660acaccaccat tattaccacc acattgataa cattcagaga gcagttaggg aatcacatta 72720ttttgaaaat tagtaagtaa tgggaaagaa ttaagcatta tcgtatatga tatgtacata 72780tttatataca tgtatacaga tcctatatgt ggatctgtaa tttagggtag tcatagagtt 72840gatgagaaag agtttttctt aagaagtatt acaggtaata aaggaaggag gaataaagaa 72900ttcgagtatc acaattttgt aaccctcaat gaattagcag atctaggcaa tcagaatcca 72960cgataaaaat tactagaaaa gacagaaagc gagtgagact tttaagttat tcctacaaac 73020caacatctat gaagtatttg ggaaggaaat aaactctgaa tctgtttttc gtttttttga 73080gatggaatct cactctgttg cccaggctgg agtgcagtgg cacgatctag gctcactgca 73140acctccgcct cccaggttca agcgattctc ctgcctcagc ctcccgagga gctgggatta 73200cagctgcctg ccaccaaacc cagctaattt ttgtattttt agtagagacg gggtttcccc 73260catgtttgcc aggctggtct caaactcctg gcttccggtg atctgcccgc ctcgggcgtt 73320tttgcatgta ttttttcagg tgctgaaaaa gctctccctg atttcatttc ttgttgactg 73380cttagagaca tctttccaaa tgtccttcct tgtcactttt tcaaagttag gagcagggat 73440tgcacgtttt tttgattggt aatttttgtt tcaaaggttt gattcagatt cagagttgat 73500tttgctaggt atggtggctc acgcctgtaa tcccagcact ttgggaggcc gaggcgggcg 73560gatcaccaga ggtcaggagt ttgagaccag cctggccaac atggcaaaac ccttatctct 73620actaaaaata caaacattag ccaggtgtag tggtgtgcac ctgtaacccc agctactcag 73680gaggctgagg caggagaatc acttgaaccc aggaagcgga ggttgcagtg aactgagact 73740gcgccactgc actccagcct cagtgacaga gcaagactgt ttaaacaaac aaacaaaaaa 73800ttaacaaaat cttatttcta gtggtttaca tattaatgat gctggcaact tttagttgaa 73860atgtattaaa cagcctcgca gtttttattt ttatgaattc tgcatacaat aaataaatac 73920caaccaaaat atctatacag gcatacccca gagatactgc cggttcagtt tcagaccatg 73980gcaaataaag tgattatggc agtaaagtga gtcacacaaa tttttttgtt tcccagtgct 74040tataaaagtt acgtttacat tatactgtag tcaagtgtgt aacagcattg tctaaaaata 74100agtacattat cttaatttaa aatcattgct aaaaatgcta atgaagtgag cacattttgt 74160tggaaaaatg gtgctggtag acctgcttga tgcagggttg ccaccaaaaa aactcagtat 74220ctgcaaagtg caataaagtg agatacgcct gtaatgtctt ttttctcttc tgttattaca 74280agcactactt taagtcttca actaccgaat acttgtagac tatttaatga attaatattt 74340tcatcatatt tatattgaga aaattaagaa gctgaattta gttagaaaag gtttagaata 74400tcatcaactc ttgaggatga gttggaagct actttatctg tcatttttca cataacacta 74460gtttgccctt caaatcatac atttaaaaat gatcttaaga tacataccaa gtagggatta 74520tgttgttata aaaagctaga agtaaaatta agactttaat ttcaaagata atttctctat 74580aaagaatagc acaagatatg ttcaactatt ctcagagatg catcaagata tgactttttt 74640tcctactaga aattatatct atgatcaatc atattacaga gatattgctg ttatactcct 74700tgtgactgat gaggggggtt tttcctatca ccatgttatc ccaacccttt aatgaatcac 74760ctttgaaact cagaacaggt agcagcacag aggttgctaa gagtaaatac attcaccact 74820gctgccagaa atgaccaacg gtgcaccaga aagaagaaat actggtgaat tcccacatac 74880cacgacgaac aaccttataa aataacagaa caaatgaata taaagtaaca tacagccatc 74940ccttggtata tgtgacaggg attggttcca ggaccacgtg tgtataccaa aatccatgca 75000cgctcaagtt ctgcagttgg ccctgtgaaa ctggcatgta tgaaagtcag ccctctgtat 75060atgcaggttc tgagtcccac caatactgaa tctgctctta gttgagaaaa aaaatctgca 75120tgtcagaggg cccatgaagt tcacagctgt gttgttcaag ggtcaactgt aatataaaaa 75180taacatttat tgctagatag agttgatatt cattctagtg tttatatact tgagtcttag 75240agcaatagcc tttggagaca acttccagga aggtacgtgt

ttgcatgcct tctcactgca 75300atccatcagt agctgccact tgaaccagtc ttagcagggg atggggaagc ctatgtcttt 75360cagaattgga tgatgtagta cacagaagta gacagtagag tagtaagggc atttgcttaa 75420aagcagatag aactgggaac ctctacatct ttctgtgaga attcagggct tagtttttta 75480gggtgtaaaa tggaaatact agtatgtatc tggacatatt atcttaagaa ttaaataaaa 75540gactaccatt tcactcaaaa agtgcttgat cctgggaagc catgtaacag ttcacaaatg 75600cagagttcaa aagaaaggaa aaattgcaga catggtagcc ttattttaat tatcttctaa 75660ccagcagtaa gcatactcta accagtgagg aatactagat aatccatacg ccaaatgcag 75720gagattacat gagacttata tataacaaca cttcattcat gaaataaatc acaagattag 75780ctctgaaaaa tggtgattat attacaaata caaatctaag tatatagatg acaatttgtg 75840agtttgctta tttgggaatt agtacctaga gataaaggtc tgaagcaaaa tcacacaccc 75900ctttggtgaa tcaaatcttc ctctgaagaa ctaagtacct cacctaatca gaacagagtc 75960cctggtatag ccaccgtcat attctgtagt ttcttctagt gcttggaaat ctagattctg 76020caaattcaag aaaatatgtc ttagttatct gctatactac tgtatcatta caaacaataa 76080atcgatacat gactttttgc agacacctgc tttcttaccc ggcttccaca tataagcaat 76140tcaatttctt ctggtctgaa taagtacttt aagggagatt cattggtcac catatgaaaa 76200cctctccgaa aagccttgaa ctgtttttct actgatttat tgagaatgta gtcagaataa 76260agattgacaa attcctgtag aaaacattaa tcacaagaac ttcttataat atgctatgca 76320aacaaaacac aagttattgg gctgcatagc tttttaattt ttccaagaaa aaaacattca 76380agaatattct taaaaattca attttacacc tacttcttaa caatttcaca attctctgtt 76440atagccaata ttaagctatg aaaaggccat atacataaaa ctgtcaagaa tattcttaaa 76500aattcaattt tacacctaca aagaaaacta cttcttacaa tttcacaatt ctctgttaca 76560gccaatatta agctatgaaa aggccatata cacaaaactg tcatacttat aaatgtcaat 76620ttcgataatg tattaaacag aactgaatat aaatgctgga gacgcagaaa attctttagg 76680ttctctcaac taggtatgta aacagacaca tttaaaccat gtatccatct atttataaat 76740gccaaccgtt ttaagagagt acaatatatt tgatttaagc aaaccaggaa gtcccaagtt 76800ttctaccata gaatatttgg taagctgcct actgtatcca gttcagcaaa aatgtggaag 76860tactgactga cacataaact actgtatcaa atcataaaag gaaaatgtgc tttgtaccct 76920tagtgtgtat aatatttaat caaaaaatta tatcaatata aacatgtttc ttaaaggtat 76980actatatatt tgtcataatt ttttgcttgt tatgttctgg gttattccca ttcccgatga 77040acacatttta gtcaatagaa acccaaatct gacgtaaagt atacttttca gaaatgtatt 77100tttatcacaa acaggatata catgtgtaaa ttaaatgtaa tttttatttg ttctgtgata 77160aagaccttaa tcatgaggtt cctgcttaca gcttaataca agaaacaccc tgcttcttgc 77220tttatactac aaccacactt agctattaac tatctcctgc ttcatgtcct ctttctctaa 77280aaggggcttt agtgcccaac tgtgtgcttt cataccaact tatatccaag atttagaaaa 77340gtggactcta gcatttacat aaacttagtt acttgttttt tttttgtaca gactatatta 77400gatacttctt taaaaattat aagcatacat gctttgaaag tgttaatgaa gagacaaaat 77460gtgacataaa aacatttatt accttcctgt tttcatttgt aattggaatt ttatcaccat 77520tttcctttag atcatacatc attgggttac caaaaagatc tgtctgtgat atctggaaag 77580tgatcatcat gtcatcttcc acattccctt catactccaa taaatctttt aaactctgat 77640atagaaccta gcaaccagaa atggtaaatt gaggccacca tagaactaca aaatacaaca 77700aataatttat atcactcttc ttagaagaca acaaaaatct tatcaataaa aattgtaaaa 77760agtgtagaca gtatccctcc agtcccccaa atagtttcca acctataaaa gaaagattgt 77820cagtcactgt gatgtcccat tctctgctga gaaaccccat accaccaatt tagcaataga 77880attcacactg tgctctatga gaaattctgt ggtgtctcaa ggtgggggtg ggggtggtag 77940gagcaggcac actcgttgta actaccaaga cccttagcca gggctactct gatttttacc 78000tatctcttaa gatttcacta aaacacaaac aaaatgttta gaaacacttc tctatgaaat 78060caatcttaca aaagcttcgt gctgacagca atatataaaa atgtccccct ttgagttttt 78120aagtgtttct ggtacttcgg tcagattaaa acatttttat cttattgata agagtatcaa 78180caaagattct aaacaaaaac tttaaaattt tgacataaat taaatttttg catttaaata 78240aaatctaaga gactgaatta aaaaaatgac aaagaactta ctgggtgaga gtctcccaag 78300tcacgaaaag ttcctttttt ccccattagc ttcctgtaga caaccatggg aaaatgtaca 78360tccagtatac agttattgta aatagccaga cccagtacta tgccaatcag agtaaactga 78420ccctcagttt caaaagaaga tggattaaac caaaacaatt ttgtagattc atcgtatgtg 78480aacataccta taagaaatga tttttaaaaa tacattactt ttgtatttta ttaaggactg 78540atgaataata caaatataaa cttaaaataa ttatgcatat aaaacattta aaatatatgt 78600aattaggcat gcaacattga tttctattat attagcactt gagaaaacaa tgtccacata 78660tatctgctat aaattggggt gaatggtgat atgtaatttt atgaagccaa aggaataaag 78720tagtaagtaa tttggtggca tcgggtagaa aaatacgtta attaaattca agctttctaa 78780ttttctacta acacataaga aaagttatat tcatgcttta tccacccata gctgaaaaat 78840aaacataaaa aaacaaaaaa tggctacatt tccaaagtgt ttcatttata aaaatataat 78900tacttatatc tgtaccacac gtactatttt tgaaaacaat ttttcatttt tttccttgag 78960atggagtctc gctctgttac ccagggtggg ctggagtggc acgatctcag ctcactgcaa 79020cctctgcctc ctaggttcaa acaattctcc tgcctcagcc tcctgagtag ctaggactac 79080aggtgtgcac caccacgcca gctaattttt gtatttttag tagagaaggg gtttcaccat 79140gtttgccagg atggtctcaa cctcttgacc tcgtgatctg ccagcctcag cctcccaaag 79200tgctgggatt acaggcgtga gccaccacac ctggcctaaa aaacaatttt taatcacagt 79260aaagaaaggt tcgttttttc aaaattagac aatggtgcca tgaaattttt ctagcatttt 79320cctaataaaa tcagatgatc tgaaatacat aaacctggat aaatctgatg aacttagata 79380aacaaagcta ttcaatacac atgttaatta tgggccaata cgtaaaagaa aagtagtttc 79440gtagtctttc aatttcacat ggtcttcact atgcaagccc caatcatgga ggcttttttt 79500tttttttttt tttttgagac ggtctcgctc tgtcgcccaa gctggagtgc agcagcactc 79560agcttagtgc aagctccgcc tcccgggttc atgccattct ctggcctcag cctccggagt 79620agttgggact acaggcgcct gcgtggaggc cttttttata tcactcattg gaaaattggg 79680gcccggtgtg gtagttcaca cctgtaatcc cagcactttg gggaggctga ggcaggcaga 79740tcacttgagc tcaggagttc aagaccagcc tgggcaagat ggagaaaccc catctctaca 79800aaaacacaaa aaataagctg ggtgtggtag catgtgccta tagtctcagc tacttgagag 79860ggtgatgcat ggggatggtt tgagtcttca gcctgggagg tggaggttgc agtgagccga 79920gacagaacca ctacactcca gcctgggcct cagagccaga tcctgcctcc ctccaccaaa 79980aaataaaaga ttaaaaaaaa ggaaaattgg aatctttgga atgagtacta ctaaaaattg 80040atttcttccc ttttaaatga ctaaggaaaa aaattttaat tgaatttaaa tcattttact 80100acatgtttag ttgctactgt gaaacaatga aaacatctta agaattaagt ctgaaaaaaa 80160tctgtaattt ccaatagaaa agtagagctt agaaatcaga aatcaaatta tacagtatat 80220aagtatttta acctgtttat aagaatttta aaatctttga aaatgtcatt gtcactttaa 80280gtttaaaaat aaggagcttt aacatgtttc cttgatcact aggagtatga attaagctta 80340ggacattttc tgaatgcaaa atggtttgaa aattaaacaa ttcatatttg agtttcatgg 80400tttctatcta aacatttttt ttacaggcca agttacaaca tccatggaaa tgcttttagt 80460tataagtatg gtattaacag gatcaagaac agctgacaaa taattacaat taataaattt 80520gttcaagctg acaatggtgt gtctaaaaaa ttaatgaatt taataaaatg tcacaaatgc 80580ccttccttgg aaccaagagc aacaagctat aagttaatca gaatataact ctgaagttcc 80640attttaggca cattttctca tgttccaggg agaaaagcac cataacctcc tggtaaaata 80700ctcctttttg atacttctac tgccatcact tcagttatct tactggaact gcttaatcta 80760tgttttttct tacaagtatt tctgcatata atgcaagtat gctttatagc taaggtttaa 80820catttctaga tacatcaaaa gctagagcta aggtttacca aatttaagaa ctttatgaag 80880tttataattt tctatctcaa aaagggcgtc gtcttatgta tctctgattt acttagaaca 80940aactacatac aaatggcata gctttttagt tattaataaa acactagcta gattatagat 81000aagggatgcg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgactaag 81060aacagtaaca gaaaggtcaa ctcaattaga atcaacaggc tataacagct caaatctcaa 81120agctgaaggg aaatgacata cattattcta tgcacactcc 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aataatctca tttttaaagc 87900tctgtgagtc aatgaataaa taaaagaatt acgttcatgt ttcactagta agagtgttca 87960ccaatgctgg aagctgggag ctgaaaatgc tggaagctgg gttagactag gctattagaa 88020accagataaa aagggccagt gacttttaga ggggattttg cagaacctcc tggtagtggg 88080gctttttatc tgtgccaatc acctttatct gtgctaattc ctatttttga acttccctat 88140tcaattccac atctcagcca ttccttttgg tttttagata tttatgtgaa atgctagaac 88200tccctgacta attggaacca agtttcacat catcatcatc ctctattcca agcttgctgt 88260ccccatgatc ctcttaaaaa tctgagtttc caaaacacat aaacatactt gtatatcaaa 88320atggcctttc tctttctctc tatatatgca cacatacaca cataaacaca catgcacaca 88380cacccccacc tgagctgaac caaaggccat cttcccaaat attggagtct ggctttatca 88440aaatatttgt ggagtattac cagaagttca gattcatagg ctctacaata cagcttctaa 88500tctaaacctc tagaggttag ggccctggat tttatttatt tatttattta ttttttttaa 88560acaattttcc cattcagatg tgaaggcagt ttgagggacc tcatgatggc aatactatat 88620tcttccataa acttataacc tctatcttct cctttaagaa gtaatttaaa aaatcttaat 88680acatactatc attggaagtg cttcatctaa gaaaaatgag aaattatcta cttgtaaaag 88740aacacacaaa tgggtatatt tacatatttg taaatatgta aatatttaca tatttgtaaa 88800tatgtaaata tttacatatt tgtaaatatg taaatatttg catatttgta aatatgtaaa 88860tatttacata tttaaattaa attaatttac atattaaatt aaattacata tttatattaa 88920aaatgtaaat ttgtaaatat ggttttacat atttgtaaaa acctcatcac attaggaatg 88980tcattcaaat gaaaggtgaa gtctaagctc atgtttatca ctgtactgtt ttctaaggaa 89040caataacgaa aaaatactga aaataagtcc taggggaact atggcagtaa gttaaacggg 89100tagggtgggg gtgttcttaa gcagttctaa ggtatttgtg aagagacaac ttatattcta 89160ttgattctaa aattcacttt tttcacatta tctctgaaat cagattacac catacaaatg 89220atagcatctt acaatgaatt gctggtattt ttctgtctta gcagtaataa aatgatagtg 89280tttcctacaa tcagagatta gtgaaaatac tatcaacatg aattcttctc ttgtcatcaa 89340agacaatttc tctaaaaacc tcaggaaaaa agctgctagt ctggtttgaa tttcacaata 89400tataaaagca atgaatatat ttttcttttt taaaaaaatc cacaattatt aattcaagtt 89460accactcctc aacagaaaac atacaagagt ctcatatatg aagtcactat tttctgggct 89520tgatttctaa actaaggagt ggataactct aaggtttctt ttagctctta aagcctagga 89580tcccataatt ttatttatca atattaagct taccacaaag tttctcattt cttcttttcc 89640acccaaccct gctatacaat tccaaaatta gcaagtcata aaaaagtggc cactagtttg 89700ttcactgtga tatataagaa tgaggcttaa aaagcagaga tccttgggaa ataagatctt 89760gaaaaattca tcacttttga gctttcatat gagagaataa tcttaaacca ctttctatta 89820tagaacttct actatgtgtt actaaaatat gtggctttat cataatcatc acctgctgtg 89880aatacttact tcaaaaaatt acacaggtta tcaattttac ttataagacc tatttcgatg 89940ctggtttttc tagtcttttc acattctgtt atttttttaa aaaaaagaaa aggaagaaaa 90000agcaagggag aaagaaagtt aagagtaaaa tctgcttagt gtgagcattc agagccccta 90060aggaagcttc tctgtaagca tcatctcttt ctattttcac agtttattaa actgcctaac 90120acacatgatt tctgaagtga actgcttttg tgggctggca atattcaagt acatatacta 90180tctagtgaat cctgatttat gataggccac actatattat ctaattttga tataattaag 90240cagaacttgc taaaaacaag ttaccataat taaagagctc acagttaatt ttacactaac 90300acctaaggct ttaaaagcag ttgtttcctt aaaattctag

aatagaattt aaaatataga 90360ataaagtata taccaagtac ttaaactttt gtttttagtc ttgctttact agagaaaaaa 90420atgggggact gaggaaaata agactgagta gacttctgag cgaaagacag ataaggaagg 90480tagacaattt tttgaaccct ggaatgattc ttacagtgta atttcattgc cttttaaagg 90540ctagacatag cttttgatga agacagctga agggatctca cctcaagaag ccaaacataa 90600agtatgtagc tttggcattt gcagttctgc tccaacaatg gatagtcata gacagacctc 90660aacatacctt tcataaaaca tcactcacat tcttataaaa taccctatca ttctgctaaa 90720ctatgtaaag taaggtgctg gctgccttat gatcagtcat taagtgtatt aattattcat 90780gtgtctcaaa actatgtaag tgaaataaaa agggtaccag aaagcaagca ataataatga 90840cttcaggcag ttaaagcccc cttttgtctc tgctagaaaa ccacctgagt tgcttaaata 90900atgcagaatc tttctccaac actataaaca tatctgacat taaaaaaaaa aaacaaacca 90960gtaacaaaaa aaaaaaaaac acacaaaatc tctgcaattt ttaatttctg agatgtcaaa 91020taactttttc caccgggcta cagctttatg aattctcgaa tttagacaat tctaaaacta 91080aaatttagac attctaaaac taaaatacat tttaaagtaa aaatcacatt tcctagttgt 91140ctgtgtcaga caactttact agtatactat agtattatta actaccttaa tttttgttga 91200cattaaattt tacttgaaga tgataaaaaa gtagcagcaa tgacagggat agctaatact 91260agaaatacaa taaagctctg cgataatgca cctgagacaa atgtaaattt tggtgaaatg 91320tgactgacga ctgctttctg tcttctggcc actcttatat tcttaaacag atacactaaa 91380attatcttgt ggaggaaaga aaaggaacaa atggcaaccc catctggaaa accaggaagt 91440taggatgagt acctgagttt ccagtatttc cctgctcaat ctccctggac ttgatgttac 91500aggcaccact aatccccaga tttttggaat agctgctgtt ttccagaagt tccaagaccc 91560agagttgtct ctatgaattt ttaagtggga agaaactacc acagccaggc agtgccgtcc 91620acacagggat attctaagtg gcccagcagg actcaactct tactggcaac aggcactgat 91680ctgccatggt cacttcatca agtccacaat aatgcaactc tgaattttac acgatggaat 91740tttttggaca ctacctataa ttttatggta tggttttact gtaccattac tcccagttca 91800agactgcaca tccacactta ttattgggta tctccacatt tctcttgttc tggtctttgt 91860ccaacactct attctcttct gaatgaaaga aactttggat ttagttttca agatttagaa 91920aacccattct ttttcaaaga tagcatgacc attatataat acaaccgata ctttgtagaa 91980cacatctata aacttgcaca ggaacaacaa aagtataata cttatataag atcagaaatg 92040tccatgtgtt cctatgctat atggtatcat ttttttcagt cacttttaaa atgaattcac 92100tgaactgtat catgatatcc ccattattag gtttttaatc tagcagccca acttacccgg 92160acaagtgcat catctatgat atggtcacgt ctaactttga gtctcaaata tggattcaac 92220tgctgtcctt gaactaagct gtagagaaca gtgattcttc gttcactgta catgcgaatt 92280ctattgtcat aatataatcc caaattcttt gtgacagcat tcaatataaa gggacatgtc 92340ataaaagaga atttgttctc tgtttctact ttgaaaaaag tataatcttt atccatttct 92400agaacctcat tcagtggttc attaataaac tcttcaaaag ggataagtgg ttttcgacaa 92460tccagggttt taacaccaag ttcagtttcc agggggtcca ctcgaggacc tttcttgttt 92520cttctttctt ctcccaaaag ttcctgaagt gtcagctcgc tggactcagg gatgggctct 92580tcatcatctt cttcattgtg atttgtgtcc acttcccctc ccactacatt tgcatagtaa 92640accattttca agcacttcga agcagcaaca atggcatcat catcattcac tagatttcga 92700ctgttaaatt cattgcttat gactttataa gtaataagtt gctgaaatgt ctccatcatt 92760ctccgaatct ggtctgcatt gtatttagac cacagtctga tcagttttcc ttgggctgca 92820aggggtagct tgctcatcgc tttgcaaaat aatggcaaag ccatttccag atattcagga 92880ctgtggagat ttctattctc cattacgata atgaacaaat tcagataatt aggatctcga 92940gagtatacat tgtgatacgt caagtcacat tccacgttag gtgacaaata tacaagtgca 93000ttgagaaagg cagtttcaat tttttcatta gagagcaatc tggtgtagac ccttctaatg 93060gcatcaatat ccacagacac atcatcaggg cctaattttt gcaaattgtt gtctccctgt 93120gagctatcac ctatccttga ggaagatgct tctgagtctt cttccatagc agcagcagaa 93180catgcagctt tttccttttc atcttcatct ttgtcttcat cttttgcttg aagagatttc 93240agttcttcct tggtgtgttg tttaactttc cggaagctct gtaccaatgc ctcagcacta 93300gaaaaaactc ttccaataac acggattaaa ggggaataat cctctctttc tctacataat 93360tcaagaattt catatacctt ctcttctgtt aagtaagtca catctagaaa atcagaggaa 93420aaaagagaac atttattttc ataatatgta tgtttactct gttgcaaaaa gttctaaaag 93480taaaacagcc aaacattcta ggctcaatat catttatgat atacaactct taaagtatct 93540aatacttaga ttcagcaaac aaaatttaat gcttacctat ttttttcaat gaactaccta 93600cctataccaa tgacctcacc catgctttta cattctttca acaatgtata actcttaata 93660agatcatgag tttaaacttt ctttataatc aagatatcta 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tggtctccaa aaattgaagc 94560acaaaaatac caaattttat catactaagg atctgatctg tggcccagct acaagagtac 94620caacacttac agtttctttt atgggcctta actctttaag aactaattct caggtttgtt 94680ctattaagaa tatataagca tcttttgcta aacatcagta acctcttccc caaaagcata 94740aaaccactgg aaacctattt ctcattattt tcattgaaaa tttttaaaat accttcttac 94800aagacaatgc aaaactccta atttcctaaa atgtaacata acagaaaata ctactatcct 94860attaggacat gaaacacttt gcttcctgag caagtcataa ggttaacaaa gacttgcttt 94920gcatgcagta atatggcctg tcctagggat caccattcta gactcacatg tcctttgttg 94980atatgtcatg ctttcggaac atcttcaagt gattctgaca gtctactaat cctatataga 95040atgtacctat actttttcca aggagtgttt tttgttgttg ttttttgaca tggagtctca 95100ctctgttgcc aggttggaat gcagtggcac catttcggct cactgcaact tctgcctccc 95160gggttcaaac gattctccta cctcagcctc ccaagtagct gggactacag atgcacgcca 95220ccacaactgg ctaatttttt tgtattttta gtagagaggg ggtttcacca cgttggccag 95280gttggtctcg atctcttgac gtcgtgattc gcccgcctca gcctcccaaa gtgctgggat 95340tacaggcgtg agccactgtg cctggcctgt agtgtttaat atgttaaact gaatttttca 95400ggtatataga taaaaattag tcaaaaaaca ctgtaattag atatgaacat tcaaaaagtt 95460tggtttattg atacaggtgt tctactgaga aagacatttt tataagattt cttgtgataa 95520atgtgtccct gaaaatactc aatcttttaa attttgcact taaaaacctc ctacgcaaaa 95580acagtttggt gcagactact taatacttat gcaactttgt aattagtgtt aacaaacata 95640attaataatc aaaataagag tactagtgta cagaaagcat ctctaatttc caatatacaa 95700tagtaactat agaacaatgc aaaaacaaaa acaaaaaaac cagtaacgaa cagggaacag 95760taacagtagc atattagaaa acagggataa ggaaaggtca caactgctat aatgcagact 95820tgagcaaaaa gaggaggggg cataaagact ggataaagcc acacaataag cacctctaag 95880atggagcacg tggccaaaag agcagatgag cacattcctc atgtaccaca tcccccgcag 95940ctggctgcat tccactgtgc cagcactcta cattcagtta ctggtactct ctggtgcaca 96000cttactggag aactgtgtac aataaagtca atgcagcttc cacattatac tgacacacta 96060tatatacaga caacatttac ctattttccc atagtgtgtg aactaattca tgaaacagaa 96120ttatacactc tgaagcaaca aagttgtgta aagtgacgaa aataatttta ttgtggaaac 96180agagtagagg ttataacaca atgccacttc actaattctt tcaaacttga ttcaaatgat 96240gttagtgaaa gttttagagg caaatgcctc taaaattctc tgacttgtat gtcataaaaa 96300aaaagctttc tctcttaaaa tacaattgcc tctgggtgtg tgtaggggtg agagtggagg 96360ggcgctaagc cctggtagtc attcaaaaat ttgattactt tgttactaat taccttcact 96420gaagaaaata acttggagtt ttagtattag cgacaaggta atcgaattct gaattttgga 96480cgtaggacca tttagaaaac aaaagacctg agtgaagact gtcattggta ggtcatgatc 96540cttggcagca tgactgtacc aaatgtccgg aaggagagtg agaagcatat atctgaagga 96600aacgtagaat tctcacataa ctatcacaaa attttgtctt gccttcaaaa atggaaaaaa 96660cccactgttt cctctttctg cttctttcat cacatcatcc tgaggttcca gaactcatgc 96720cagatactta gtccagttat tctataactt aaatagatgt tgtggcctag ttttggagat 96780tcactaccac ttaatacatt tttcctaaga attttaaaaa gttcaaatca atctattaac 96840aaatgaactt ttggcatatg atctgcttct aattagcaat gattagacct gtaaaatgta 96900gttattattc ctgtccgtta ccacaataaa aaattggttc tacgatatca aaatgtcttt 96960atgtcacaga agaaaaaaca aataaaaact aatacattta aatctcccac atggttttca 97020ggcaacaatt ctcaattgaa aataaaacat cttaccttta aaatcaattc tagcgccttt 97080cttgttcatt tttatctcag agcaggagtt gttgggggca cctttcgagt tctcaaggta 97140agctgagctt gctcctttct tggagggatg aggatcacag agttttgcat taatcttata 97200aagctcgagg gctttaatag ctgctgcatt attatccata cgaagaaaag ttggacagga 97260agcacaaaac tcattcgtgc aggcttcatt tccacagccc tcagttaact ggtggtagta 97320gcgttctatt agatgctttg cagctgctcg cttcctgtac caaacattca aacaataagc 97380acagtgatta gtacagctct caagtacaaa gctcaacata tcatcaaggc aaaagttagt 97440caagcactga agtgattaac ctgtgtatga agatgagaag tagaaaatgg agatgcacta 97500tttaccacaa ctcctgacta gtgaaatagt taataaatat tttctaggat attatgataa 97560agaatgtcaa tgcttactga ctgtattaac agagtataca ttcctttggt ttaaaagggg 97620ggaggggggc ttgtctgatg gtatatgtgc ttaagttgtt tagtacaaca gctgaaaaat 97680cacaaggttt acagctaaga gttgtaggat tctatcattt taaaaattag cattaggaaa 97740atgagtctat ttaaagcatc aatccggttt tcttttacat aatcagtatt tcatttttaa 97800cagcttgtaa acatttgact tatagttaac acagaatatc tatctggaat aaaaactatt 97860tttcccaatt atcaacaaca tgggagactt aaaaataaat ttgtaagtgg aatatccaaa 97920gatagtgtta cactgaaaga aagcggctgc agcctgagtg aggcagatca tgactgtaat 97980caatcccata actgtgggag gctgaggcag gccgactact tgagcccagg aggtcaacac 98040cagcctgggc aacatagtga ggctactctc tacaaacttt tttaaaaaaa attagccaag 98100tgtggtggca tgcacctgta gtcccagcta cttgggaggc tgaggatcac ttgagcctga 98160gagtcaaggc tgccataagc tgaaattgca ccactgcact ccagcctggg caacagagct 98220agactttgtc tcaaaaacaa acaaacaaac aaaaaccaaa aagaaaaaga aaaagaggct 98280ccaaagtgta gaatcttaaa tctaaaactt gtcactaaac ctcctcatta gttccacgtc 98340acaagatgga atattttaag ttccatcacg tgaaatctta tattcctttt actctgtcaa 98400cttaaagtac acagattaat gtcctattat ggctgtttca ggagagctac caaaactgag 98460ggaggaatta tgtgaccatg taagaagtta gatgacatta tattcaacac taaagagtct 98520atgcttctac taactgataa aattaccata atttcagtag aataaactac tggatccaaa 98580gaaacataag tcatagccat tccttttggt cccaaattca caagcatgga aataacaact 98640acaaagaatc aaaattataa gaaaaatagt tcagctgatt ttcaaaacat tcacagaatt 98700atctaacatg taggactaga tgaccaatgc cagagtttac atcttgctaa tcatgagaaa 98760ctcaacaatt aacaaggcag caagtaagtg aagaaacaca cgagatccaa ttttgtctca 98820acagaagaac tttcactctc tgtccaggta ggtgacaaaa agtgataatt cacacacatt 98880caattcattc tggtgcttat ttttcaaatg ttccatgcaa atttaccact cactgcatgc 98940aaacagagaa aaaagaaaac ccacaaagca catgtaaaaa ttgcataaac atgacttaaa 99000gacattttgc tatctatgaa gctataaacc ccacctgaaa attaaaaact gttatcacag 99060atttagcact atgcaaaata tgaaagtgaa aacatgtaaa aataaaatgg aaatgcagaa 99120tctagaaaat ttaaaatata tattgctacc cttcagtaaa gaaagggaga taatgtcatg 99180ttctcccaaa ctacagtgac ttccctggtg aaaatgtaag taggtatttc ctttctggag 99240gaaaacagtg aacatgtatc gaaagtctta aaaacacgaa taccgtgtga atgagatcct 99300acttgtggaa aataatttga caattttaca aaaatgcatt gtttctatat ctgaaaaaaa 99360catgtctaat aaaagattac ttaaatgaac tgataatagg tcacacaata aaataaaaac 99420tatcaagatt tgaattcgac aaagaactgc ctagtcttcc tgatatatca ctaactgaca 99480aaagtcagtt aaaaagtagc aggtgtagga tgaacccatt gttatttcaa agcattaata 99540gcattttctc tggatgatgt gattaagtga gttttaaaac ttcttccatc ttttgatttc 99600ttatttattc tttccatagt aaggatgcta cttgcatatt aaaaattaat aactataagg 99660ggaaaacaat agcagttata aaacttcata tacatgattt gaatggcccc gagatttact 99720aaattgtctc tgatttttaa aaaaattaca aaagtcaaaa taccaaaata gatattttta 99780atgtgagctt ttatactaaa ataacctccc caaaacttgt cagatacata gtacttcttt 99840atatactaca taggaggaga gtgatcagca caaacagaac gagactgaaa aatcagaaga 99900tctgcattaa gtgtcatcct tctgtcccta agcagctgta ggatcctagg gaaacaattg 99960tctgggcctc agttttccca gctttatgag tcccttcagt tctaatttta aagttatttt 100020atgtaatagg gaggtgagca agaaactagt atttgctgga tgcctgataa cttattacac 100080acactgcggg gcactttgac atgggttatc tcatttgaat accatgacat tgaggattat 100140cttcagtcca cagccaaaga gagaaataga actggagtca aatcacgaaa ccaagatctc 100200aaagaaagta cagaaccaag acaagaatag gagtccagga gtctttcagc ttcagagtct 100260aagtcatttt gactatattg ttttgcagaa ggtgattcat ttcaaaataa ttatttttta 100320acatgagtaa tactgaagaa ttctatggaa aaaaatctca taactaatgt gcaactggga 100380aaaaaatctg aagaatcata acgtgaacct cacatttatg catctatctg gtaccacaaa 100440ggcagaccaa gtttcttgag gaaatggaac cactatattc tgcaaagtta atttctctct 100500aatttgttga ggacctatgt gctatgcatt ttatagacat tcccacttta gaggggaata 100560aaccttaaca gttaagagct cgcattctga ggccagatgc ccaagttcac ataatggctc 100620tacctcttaa ttaactatgt gaccctgaat gaattacttg atctttctgg acgtaagttt 100680cctcgtaatt taaatggtag tatctacgag cattatcatg gtgagagtat tattcttctt 100740cacattacca attgtgagaa aattaaatgt gttaatatat ataaagtgct tagaacattg 100800cctggcacat actaagcact atgaaagtgt tagctactac tattattgtt gtaattgtta 100860ctatcaccct cacaagtgta tgagttagca ctataaacct tcattctgct gatgctcaga 100920gaaattacat aagtcataca tctaataaga gatttgaact cagctactaa acttgagctt 100980ttaataacta ttaagaggag gatctgaaag tagccctcta tatttgatct gcctttctgt 101040gccaagttta acaaatagca gctacactga tgagacagga ctaaaggaag ccatgccaga 101100acctccaagt taatggtgga atcaactata ttaagcattc ttcttttttc ctcactccaa 101160tgacacctta atttatctga cctcatctgc atcactatct aatttaggat atgtaaaagg 101220catacaaaat ttagagcaca taaaagggcc tctacgttgg aaaagtatac actaataacg 101280actttttggt atagttcctg cgcaagggaa atccttgtac catatgggtc accaaatcat 101340agctcgtaag tcaaatcgag tccaccacct gtttatgtct ctcaagctta gaatatttta 101400catttttaaa tggctggaaa aagtttgaaa aataataata gtttgtaacg tgaaaattat 101460aagaaattca agttccagta tccatcaagt gtactggaac atacccaagt tcattgattt 101520ataaatatac tatatatgat aatgctttgg ctctgtgtcc ccacccaaat ctcatcttga 101580attgtactcc cataattctc atgtgttgta ggagtgaccc cctgggagat aatctgaatc 101640atgggggcag tttcccacat gctgttcttg tggtagtaag tctcacgaga tctgatggtt 101700ttatcagggg tttccgcttt tgcatccttc tcattttctc ttgtcactgc catgcaagaa 101760gtgtctttca cctttcgcca tgattctgaa gccttcccag ccatgtggaa gtgtaagtcc 101820aattaaactt cttttttttt tcccagtctc aggtacgtct ttatcagcag cgtgaaaaag 101880gactaataca atatactata tagtttatgg ctgtttttca tactacaatg gcagagttga 101940gtactttagg cctgcaaaac ctaaaacaaa taccacctgc cctttacaga aaatgtttgc 102000caacccttag tttaggatct attatttatc tctgccctcc agtgaccctg ccatctaatc 102060atttggcctc ttaactctct ctttaccctg agcaaatccg taaaataaaa ggtatacctt 102120tccccaagta ctattaaaga aaagaagagg agaggggaaa agggaaaaag gggaaaagaa 102180agtgaggaaa gggagaggat gcaaaagaaa aagaaggaaa gaaaggaatt tccctgatga 102240gatcccttag taatacctta tcagctgttc ataatcaaaa ttcctagaag aggaacaaca 102300gcttacttgc tataatttct tcacattcca ttgacttttc ttattactca aaaaacaaag 102360tatgttataa tgtgttgatt tttaaaataa aacaaacttt tcttatttag tcagcaatga 102420aggtcaagtg agaccttcta gtaaaagtcc tctaaagtca gacacatgaa aatacttatc 102480catttaaaac agacaatact ttccaattat ttatttctat gattgaatta atgggacagt 102540gctacaaaca gaagtcctat ggaataagtc atacttggaa gatcagagtt tactctagac 102600aacttgacag caatcagttt aaaaatggtt tcacctagag ggatgtgtac taatgttagc 102660aattaacttt gaaaggcata aaaattaaga tggattgatg gatacaagga tggttatacg 102720ataaagcaat gtaagtatta atggcagaat ctaggtggca ggcatatgtg tgtcagctgt 102780aaaatttgtg ttcaatgttt ttttagttcc cacatatcag tgagaacatg tgatatctgt 102840ctttccgtgt ctggcttagt tcactaaaaa aatgaattta caagacaact ggaatataag 102900caatgaatac aagcactaag aagatgaagc catagaaaca gctgccagaa agtgaagatg 102960tgacagtgaa cgccaaacaa gtaagattag aattacaatt agaatcagaa atgacaaaaa 103020agaaacatga actgtgagat gcaatataaa aactaatggt gaacaaagca aagaataaaa 103080aataaaaacg gaaagattaa aaggaaaatg tgactgtaag tagagtgacc tttatattat 103140acagaaggta aagcagaatg aacatgtgag gaatctaaaa aacagatata aaatagaaac 103200aactgaaggc ttatataatt agtctataaa gctaggaacc actgcttagt aatttctcgt 103260aagtggagtc tctttgttct gagacgttgc aaaactccat tccatctcaa agttattttt 103320aatgttaaaa tttgtaagag tgagcaacgg tatcctaaat agttctaggc cttgtagaaa 103380tagactgtaa gagacatctg tgacacaact atattcagta tgatgtaggg tctgttacaa 103440tgggatgaag tgaatttaga ttatttcaag actaaaccta caattgtgaa ctcattagta 103500ccatgaaccg aacaaaaacc tattaaatat agaacaagac gattttcatg aaaaaataaa 103560tatgaacaaa tcagtatttt cagttgtttg aaatataggt taaccttcaa ttactgtttt 103620atggccgggt gcagtggctc acgccacgcc tctaatccca gcactttggg aggccgaggc 103680aggcggatca tgaggtcagg agatcgagac catcctggct aacatggtga aactacgtcc 103740ctactaaaaa tacaaaaaaa aattagctgg gcgccgtggt gggcgcctat agtcccagct 103800acttgggagg ctgatgcaga agaatggcat gaacccggga ggcggagctt gcagtgagcc 103860gagacagagc cactgcactg tggcctgggc aaaagagcga gactctgtct caaaaaaaaa 103920aaagaaaaga aaagaactgt ttttttcatt tttatagtct caatatccag aatgcgtgat 103980atatgataaa aaaaaaagta aaattgtaga actaaatatt tacataattc atctgtaaaa 104040tgaaaatctg ttttacagtg tcatatttca cagttagaaa ggaagaaaaa ccttaaatca 104100acaaattaac tttacatctt atggaattag gaaatgaaga acaaatgaga ctcaaactta 104160ccaaaaaata aaaaaaataa aaataaatta cagctaaaat aaaatagaga aaaatagaaa 104220atttttgcaa tagaaaaaaa atcaacaaaa ttaggagtag ctgttttatt gaaaagatca 104280acaaaactgg caaattctta gattaagaaa tgaagagaat agacacaaac agcaacaact 104340ggaaattaga aaggcgatac aacaactgct gccacagaaa taaataggac cttaaaagac 104400taatatgaac aactatgacc caaaaaaatt tgataacctt tgagaacgga tagacacatc 104460aaacccacca agactgagcc acaaatacat acaacttctg aatggaccta taactattag 104520agattgaatc agtaactgtc caacaacgcc agggcttcac tgaaaactct accaaacatt 104580taaaaactaa caacccttct caaactcttt gaaaaaacta gaggacagaa cactttcaaa 104640atcattttat gaggtaagca ttacttagat atcgaaatca gacaaattta caagaaaaaa 104700gaatccctaa taaatactga tgcaaaaaaa atcaacaaaa taccaagaaa cagaattcaa 104760caatgcatta aaaagattat atttcataaa ttctcaactg gaatttattc ataaaatcca 104820aggatggttc aacatacaaa aaaatcactt taatacactg cattcttagg acaaaaacca 104880ataatctcaa ctgactcaga agaagcattt gacaaaaatc aacacccttt taggatttaa 104940aaaaaggcca gacagggtgg ctcatgcctg taatcccagc actttagaag ggcaaggtgg 105000gtggatcacc tgaggtcagg agtgtgagac cagcctggcc aacacagtga aaccctgtct 105060ctactaaaaa tacaaaaact aatcgggcat ggtggtgggc gcctgtaatt ccagctactc 105120gggaggctga ggcaggacaa tcacttgaga acccgggagg cggagctggc agtgaggtga 105180gatagcatca ttgcactcca gcctgggtga caagagcaag attctgtctc aaaaaaacaa 105240aaacaaaaac gctcaattcg ctagaaagat atggaaatta cttcaacata atgaaggcca 105300tatatagaaa gaccacagct aatgttattc aatgatgaaa aactgaaagt tttccctcta 105360agatcacaaa tggaacaaga atgcacacat tcaccacttc

tattcaacat actgttagac 105420atcctgccag agttagcaac tgggcaagaa aaagaaatga aaggtattaa atgagaaaag 105480taagaagtaa aattacttct gttagcaaat gacatgatct tttatgttga aaactctagc 105540ctaccaaaaa gaaaaaaaac cgttagaata aaggaattca gtaaagttac aggatataaa 105600aaccaatatt aagaaaagag ttgcatttct aaatactaaa aatgaagaat ccaaaaagga 105660aattaatttt ttaaacccat ttaaaatgaa atcaaagagt aaaaaatact tagggaatat 105720tttcaccaag caaatgaaag acatgtacat tgaaaattaa acaacatagg ccggacgtgg 105780tggctcacgc ctgtaacccc agcagtttgg gaggccaagg tgggctgatc acgaggtcaa 105840gagattgaga ccatcctggc caacatggtg aaaccctgtc tctactaaaa atacaaaata 105900aattagctgg gcttggtggc gcgcgcctgt aatcccagcc actcgggagg ctgaggcagg 105960agttgtcact tgaatttggg aggcagaggt gcagtgagcc aagatcacgc cactctactc 106020cagcctggtg acagagtgag actctgtctc aaaaaaaaaa aaaaaagaaa aagaaaatta 106080tacaacaatg ctgaaagaaa ttaaagacaa tacaaataaa tggaaagaca tccaagttta 106140tgaattggaa gatttagtac tgttaagatg gcaatactac ccaaagcaat ctgcaaattc 106200aaagcaatcc ctatcaaaat cccaatggca ttttctacag aaacagaaaa aaaaaatctt 106260aaaattaata cagaatcttg aaggaccctg aatagccgta acaatctgga caaaaaacaa 106320agctagaggc ctcacattcc ctgatttcta aagatattgc aaagctatag taatcaaaac 106380actatggtgc tggcataaag acagatatac agaccaagtg agcagaagag agaacacaga 106440aatagggaaa agaggttctt caacaaactg tattgggaaa tctggaatct aacatgcaaa 106500agaatgaagt tggagcctta tctcacacca tatacaaaga tgaactcaag ctggattaca 106560gacctaaact taaaacatcg aattataaaa ctactacaag aaaacatgga gaaaaagctt 106620catgacattg gacttggcaa tgatttcttg aatagatcac caaaagcaca agcaaccaaa 106680gcaaaaatag acaaactggg gatacataaa ctcaaaaatt tctacaaagc aaacagtaaa 106740tggagtaaaa tggcaaccta caaaacagga gaaaatattt ctataccatg tatctgttat 106800ttggttaata tccagaatat ataaataact cctacaattc aacaacaaaa aatctagtta 106860aaaagtggac aaaggacttg aatagtcatt tttccaaaga atatatacaa atgggcaata 106920agcccatgaa aagattctca ccatcactga tcagtaggga gaaatgtcaa ttaaaactgc 106980aatgatacat catctgaaac cattaggatg gccaccatta aaaaaacaga aaataagtat 107040tggtgaagat atggagaaac tggaaccctt atgcaccact ggtgaaaatg taaaatggtg 107100ccaggcactg tggaaaacaa catgaaggtc cctcaaaaaa tttgaaatag aattacctcc 107160agttccacat atgaagagct tggaagtcac cactccatcc taacaacaag taaaaaatga 107220caaacttaaa aataaacaaa tctgagatag gataagtgag gtcacagggc aaatgactgc 107280ctgtgagagc agagaacagg tgaacacata gcatcaccat ttatctgaga tgaaaaacac 107340aagcagaaac ctccacgggg taggaaatcc tgaagtgtaa tagacaaatt gatgaaggct 107400cagtgtggac aactctgaaa gctaaaaact ccagagttac ccaattatag ggggagccct 107460acaattttct aagttttaca tccaggcaat tgaccagctt ctcacagtaa atataggaga 107520aaaatcctca accctcagca ggggaggcaa agagaaatga ttttcaagta cactagacta 107580ttccagtctt aacaaggttt gtcctcagga gaaactagtt aaccagagcc taacctgctg 107640gggttgtagc aaagccaaaa tgatgtgggg gaagagaaat aacccaactt caggccatta 107700tagctatctt gttctaacta agggaatgag ggaggggtgg gattgagaaa cacttgtgaa 107760gttcacagtc cagaggcata agctcactaa aaagactgac ctaatcataa gactacagaa 107820ctcttctcct ctccctacac cttacaacca cattactaaa tgctcattta caacagttcc 107880ttttacctag tacatcatgt ccaagctatc aagaaaaaaa ttacagggca taataaaagg 107940taaaaaacaa gcaccaggac cagatacagc agggatttgg aattatcaaa cttggaatct 108000aaaacaacta tgattaatat gctaagggcc ctaacgaact gagtaaacaa catgttaaaa 108060aagtagagat gaaaatccta agaaccaaaa ggaagtgcta gagataaaac acaaacaaaa 108120acaaaaaaca gaacactgta agagaaatga agaatgcctt tgatgggctt attagtaggc 108180tgggcatgga tgaagaatct ctgagcttga aaatatgcca aaaaactaca tctgggcata 108240aaacgttgta attacagaaa ttcaaggata agaaaaaaat ccgaagtaag ccagagggaa 108300aaaatcacct tacctgtaga ggaaaaaaag aaaaaaaaga aaaaatacat gtgacttcca 108360acaagaaaaa gagtgaaata ttcatagtgt tgagagaaaa aaaaaccata aacctagcac 108420tctaccttgt gagattatta ttcaaaagtt aaaaagaaac actttctcag acaaaaatta 108480aggaaatttg ttgtcagtac acctgccttg caataagtgt tgggaagttc agaaactgaa 108540atctacatat atagagcatc aaaagaagga aacactgaaa gtaaaataaa accttttatt 108600tttcttatac ttaattgatc taacaaataa gaatttgttc aaaataatgt caacaattgt 108660gcttatgcat acaaatgctc ctaaacttac ttattgggtt gtgtcccaat accattataa 108720attgaaaata tcatgaaaac acaacttatc caaatttgtg ggatacagtg aaagcagcgc 108780ttagaaagaa acatacaaca ctgatgcatt tattagaaaa gaattatcta ggccgggcgc 108840tgtggctcac gcctgtaatc ccagcacttt gggaggctga ggcgggtgga tcacatggtc 108900aggagatcaa gaccatcctg gctaacacag tgaaaccccg tctctactaa aaatacaaaa 108960aaattagcca cacgtggtgg caggcgcctg tagtcccagc tacttgggag gctgaggcaa 109020gagaatggcg tgaacccggg aggcggagct tgcggtgagc cgagatcgag ccactgcact 109080ccagcctggg cgacaaagcg agactccatc tcaaaaaaaa aagaaaagaa aagaatgatc 109140taaaaatcaa ttatctgagc taccaactta tgaaactaga aaaagcaaat taaatccaaa 109200tagaagtaat aaaaattaga gcagaaatca atgaaattga aaacaagata tcaatagaga 109260aaaatcaaag aaatcaacta gttctctgaa aagatcaata aaatcaataa gcctctagcc 109320aggctaagaa aaagagacaa aggacaaaat aactaatatc agaatgaaaa gagtggacat 109380cattacagat ctcatggaca ttaaaaggat aatcaaggga tattatgaac aactttacgg 109440ccacgaattt ggtaacctag atgaaatgaa tcaattcctt aaaagacaca atctattaga 109500actctaagaa gaaataatct tgattaaaaa gcatatatta aagaaattaa atccataatt 109560aataatgttc taaaagagaa ggcatcaggc ccagatggat tctctgatga attctactaa 109620acatttaagg aagaaaccac accagttctc tacaatctcc ttcagaagac agaagcaaag 109680gagatgctcc tatctcattg tatgaagtca gcattactct aatacccaaa ccagacaaag 109740acattatcag aaaagaaaac tacagaccca tatttgtcat gaacaaagat gcaaaaatct 109800ttgtattagc aaagtattag caaactaaat tttactacaa tgtataaaaa gaagtataca 109860ccagcacaaa gtaggattta tcccaggtat gcaagactgg ttcaatattg gaaaatcaat 109920taatgcaatt caccatatca acagactaaa gaagaaaaat cacatgacca taccatagat 109980ggagagaagt atgtgacaaa atttaacacc cactcatgat agaaactagt aaactaggaa 110040tagagggaac accttcaact tgataaagaa cgtttacaaa aaacatacag ctaacaccat 110100ccttaatgat gagaaactag aaactttctt actaagatca ggaacaagaa aagaatatcc 110160ccacctcacc actccatact ggaagtccta gctaagacaa gaaaaggaaa taaagggtat 110220actaacgggg aacaaaaaaa tacaactgcc tttgtttgca gatgaaatga ctatatgtgt 110280agaacataga caggaattga aaaaaagaaa aagaaaaaca acaaaaacct cctagaacta 110340ataagtgatt atagcaaagc tgcaggatac aaggttcata taaaaaagtt aatagctttc 110400ctgtaaacca gcaatgcata agttgaattt gaaattaaaa acacaatacc attttacatt 110460agcgtgtccc aaaatgaaat atttaagtac agatctaaca aaattagtac aagatatatg 110520aggaaaaatg ttttagaact ctgatgaaag aaatcaaata actaaataat gaagagctat 110580tccatgaccc gtgagagaca actcaatatt gacagaaaaa gtcagatgga cactactgat 110640ttcaagattt actgtaaagc tacagtagtc aagatggtgt ggtactgaca aaagaactga 110700caaaacagac caacgaaaca gaatagagag cccagaaatg gacccacata gtcaaaggag 110760caatggcagt atttgacaaa gaggcaaagg caatacaatg aagaaaatag tctttttcaa 110820taaacagtgg taataactga acatccacat gcaaaaaaaa aaagagtcta gacacaggtc 110880ttacaccctt cacaaaaata aactcaaaat aaaccagaga attaaatgta aaactcaaaa 110940ctataaaact cctagaggat aacataagaa aaaatctaga tcttgggttt ggtgatgact 111000tttagatacg gcaccaaagg taccaaccat gaagaaaaga actgataagc cagacttcat 111060taaaattgtc tacttgtttc ttaaaaattg ttgtcttttg caaaagacaa tgtcaagagt 111120atgaaaagac aagccacaga tagggaggaa atatttacat aagacacatc tgataaagaa 111180ctattaacta aaatgtgtaa ggaggctggg tgtggtggct cacatcccag cactttggga 111240ggccaaggtg ggtggatcac ctgaggtcag gaggtcgaga ccagcctggc taacatagtg 111300aaaccccgtg tctactaaaa atacaaaaat tagccaggtg tggtggtgca cgggtataat 111360cccagctact cgggaggctg acgcaggaga atcacttgaa cccaggtggc agaggttgca 111420gggagccaag actgtgccac tgtactgcaa cctgggtgac agagcgaggc tctgtctcaa 111480cagacagaca gacagacaga caagacagac agagacagtg taaggaactc ttaaaattcc 111540acaagaaaac aaacaaccca attaaaaaat tggccaacaa ccttaacaga cacctcacca 111600aagatataca gatggcaatt cagcatatga aaagatgtcc cacacatcat atggcatcag 111660agaaacacaa attaaaatta taagatacct ctacacacct atcagaatgg acaaaattca 111720gaacactgat aacaccaaat actggtgaag atgtagagca aaagaaacac tcactcattg 111780ctggtgaaaa atgtaaaagg atagcgtcat attagaagac agtttggatg tttcctacaa 111840aactaaacac ttttaccata agatccagca attgcactcc ctagtattta cagaaagtcg 111900ctgaaaacct aagtccacac aaaaacctgc acaccgatat ctacagcagt tttattcata 111960attagcaaaa tgtggaagca accacaatgt ccttcattag gtgaatattc attcacaaac 112020tgtgatatgt ccagaaaatg gaaaaagaga ggtactaaca acctatgaaa agacatggaa 112080gaaactttaa tgcatattag taagtaaaaa tagtcaagct gaaagggcta tatactgtat 112140gattacaagt atataatatg ctggaaaagg caaagttaga gacacagtaa aaggatcggt 112200gattgccaag gagtgggagg gaaggaaaga taaataggca gaacacagag gattttgggg 112260cagtgaaaac attctgtatg atataatggt agatacatgt cattgtactt tgttcaaagc 112320cacagaacgt acagcactaa gaatgatccc taatataaac tacggacttt gggtaattat 112380gatgtatcaa tataggttca tcagaaagac atgtaccact ctggtgggag atgcacactg 112440gctatgagag aatccataca tgtgtgtgga aggaggtaca ggggaaatct ctgtaccttc 112500ctctcaattc tgtgaaccta gaactcctgt aaaaaaaaaa aaaatctaaa aaaaaataga 112560atacatatga tccagtaatc ctacacctgg gtatacttcc aaatatatta aaaacaggtt 112620ctcaaaaaaa tatttgtata cccatgttta tttcaacatt acaatattca agaagtggaa 112680ggaacctaaa catccatcaa caaatggtta aagaaaatgt ggtatacata tataacaaaa 112740tgttattcag cctaaaagaa aagaaagaaa ttctgtaata tgctacaaca caaatgaacc 112800ttgaggacat tgctatgtga aataggccag tcaacaaaag acaaatactt aatgattcca 112860ctgacatgaa gtagccaaaa cagtcaaatt ttagaggcaa aaagtagaat ggcatttgcc 112920aaggaatagg aagaaggggg aaaagaagag ttgttcaatg aatgtagagt ttctgtttta 112980caagatgaaa aagttctaga gatctgctgc acaacaatgt gcacagttac cacaattgta 113040ccgtacactt caaatcatta atatggtaaa atgtgttgtt tttgacaatt tttaaaactc 113100taccccattg ttttctttac ctcttatata aactgataaa aacctgtttt atcttaatca 113160attattgtat gactactgct ccccagagaa aaaagtgcat gtgaaatact actacttatc 113220catctcctgg ctctgagcag tacatgtcag gaacaagagg gaattgtagc tggaagacag 113280tagaagagcc ataggacaag tctctaagag aaggaagaaa aggcgcaatg aaagaaaaca 113340agtttaagat agtagaaaag cattcaaaga cggcaacctg aggtaaggtg ttttcactga 113400agcttttggg gtaaaacagt tttgagttgg catgcctgta gacatgggaa gcagcagtga 113460tgcagaggtg aacattactg tagataaaaa cggatgggaa tttgacttgc tagggactga 113520ggggttaatc atataaataa agatggcagc agatttggga aagagtgaag aagaataagt 113580tcaggtagca gtcctcaaaa accgagaaga aactgatagc tcactgaagg cagacagagg 113640aagacaaaac cagaaggcat aagcttcaaa ggagcaggga tttttatgct gagagagtaa 113700aaggaatagt tcaaagcagc agtggagaac acgaataacg ttccatgtgg gaaaagaaca 113760tgtccagagc ttactgtacc tataacctag cttctgtaac tggaaatgaa gaatctgctc 113820tagtcaggtc atcacttttc actacttatt ttacttggcc ttcctaagaa atatagacct 113880tttggaaatt attgactttt gggtctagga cactatttct tggtttcctc ccttctctct 113940ggctgttttt cagagacttc ccttccaaat ctagagacat ttgtattctc cagggatcag 114000ctattagata ctttcccttt taaatttccc tttggacagt ttcatctttc aaggattgag 114060caataactaa atgacttcca aatgtctact tcagtataaa ttctgcttgt caggctacat 114120aaaaagggct cttcaaggaa ttatgatgtc taaagaaaga gaaagccacg atttcaatat 114180cacagagatg actacagagg aatagcaata aaaagagagg caacttagga gaggtgaatc 114240acaaaagatg actaggagct gatgacacta tgggtgggaa gaatgtttca gacaggggaa 114300gaatgtttca gatagaggaa aatgagtaaa agcacagggg cctgaaaaca tgatattata 114360agacaattcc aagcacttca agagcctaaa gttcaagaaa agtttcaaaa atatgaaaat 114420ggagaggcag ggaaaagcca gatcatggaa gacctcaata gccatgataa gtaattcttt 114480ttttagccaa aagaggagta atctaataaa agctaaagat cttctcctca taaaaataca 114540tgcacacttc aggagtgcaa atgcactctt ccggaagatt cgtaacccac tacattcata 114600aaccattaga taagaactaa aacacaagga gtcactgaag ggttttaagc aggttaagtg 114660actgacaaaa tttgcctttt caataactga aaccaaagtg ggcaaaactg gaggcaagca 114720atccagttag gtaatgacta ctgtaatagt ccggacaaat aatgtagcag taggaagaaa 114780aaaaaagatg aaattgaccc aggaaatatt taaaataagt agtttgtaag aggtaaataa 114840gctggaagag ccagaggtaa tgggtagggt ttctggtatg ggcaacaaga tatgctacaa 114900gctgagatgt tagtaaaaac aagcgttcat cagcatttag taagtgattt atttatatta 114960tatcactgaa tactcaatta tgttaagagg cagacagcag ttaaaaaaca aagaactcgg 115020tgaatcaaaa agttctgcta atttggggaa atagcatccc ttttattttt gttaatctct 115080tttgggtttg atcctcattt atagctgaca agaagttcta tgtactaaag gtaaactatt 115140ctcattaatg ttcccaaaaa tctcttggaa taattaagat ataaagaaat tcaaaattca 115200catgattata aaaatctttc cttagataat ctcataatat agccatgttt tgctcattct 115260actttgagaa gattcagcca gaaacagttc atccattcaa ctatccctac tgtgaactag 115320aagtatgttt ccttacattt gaaacagaag atataggttt atatgatgct tcaggtggta 115380gaaaagcagg gcagactatg actcgtgtca cttccttcca cacgtgatgc ctttcatggg 115440ctcctgtgtc tgtcagaaga aagcaaaggg ccttagcgtg ctcctggctt tctatatcca 115500aatgcatttt ttcacacctt ctcccatcag ctaactctac attactgcaa tacggaactt 115560cttactactc agtggacttg ttctgctgtc ttctctgatt ccaggtgtta ttcttcctgc 115620ctagaacact atgattctta gctattccca agcatcctca aggtttcagc ttatatacca 115680cttttaagat aattaggtat tcttcctctg tagtctcata ccaaccagta cctattctat 115740tatatataat aactgcctat taattaaatg ggaagctgag gtatatgacc taataactta 115800tttactgtcg tatcctcaga acctagcaaa ttacagagtc tcaacagaga tttttcaatt 115860tctcttctga tactgtctcg ataacagtgt caattaaacc tttctctaat tatttgacct 115920tgaagggaaa tagatgtcag actacctcct ggtttcgagt atactggaag catacaaaag 115980gaccttctca atctggagac caagaatatg atatgagaac ctataggaga accacttatt 116040tgattaaaga gatttctttt acttcagaaa atatttcagt atgttacttg acccataaat 116100gcttatagat gtgtaagtac accgaagaag tcatatagga aataaaagca tccaagctat 116160atttctatgg tctactctga ttaaaaagct cagctctcct atcaaattca agataatgca 116220tgtggattat ggagcacagt cacttgctac atggtaaatg ctcaaaaaga tgataggtgg 116280tattacccac attctacagg ctgtgtacat acttatcagt ttatatatat acataaactg 116340atcctaaaaa attcttccta catttctgca agataattaa aaaggcagaa acaaacattt 116400tgcataaatt tactagcttc attctttcat gtaggaagta gttactgact acctactgta 116460tgccaggcac tgtcattagg cttccattct agtggtaaag aagcaaaaca acaaagaaat 116520caatatgcaa gaagtcaaat gacattaaat cccatgaaga aaaatgcagc aagttaaggg 116580gaatgcagaa gaacatgatc attctttgat gcagccggtc aggaaaagca tcatttgagc 116640agagagctct ctgaatcaag tgaggaagga agccacatgg attatcacag agaagggtca 116700cagggtcaca gacaatagtt acaagcctta agatgacaaa gatacagcca ttttagacag 116760agatgagcaa gtgcggtaga gagatcacag aaatgaaagg aagccagttc acacagggcc 116820atgtaaatct ccagatttta cttttaagat gggaagcctc tgaaaagttc tgtgcatagt 116880aaataacatg gtttaactca gattttaaaa agatcattcc ggctgctact agagaataca 116940ctgtaaagca caagactcct gggaaacaat ttctttcttt tgccaaaaga tgatgccttt 117000aaacagagta aacagaagta taatggttaa aaaaaataaa atgaattatg ggtatttgga 117060aggtgtagcc aacaagattt accaataatt tgagtatgtg acatagagaa attaaaagat 117120gcttccaagg cttctggctg cacaggaggg gaaaaaagct gttattgact gagatggcaa 117180aatattatga gagacgtagg tttgggatga tgaaaatcag gagctccact taggacaggt 117240taagtttgag aaccagactt aactgttgga taaatcagtc tacagttcag agggcaagag 117300ctgacgatgg aaatagaaga gttgtcaata gacagagtcc tggggcattt ctgacattta 117360cagtaaactg agcatttatt aaaactaact tgctttactg aaccataaat cacttgttgt 117420atttacccct gctccattta tagttataca tctggaaaca tagccttagt gttagaggta 117480aagagtaaat accaaacata aaaaatctga tctggttggg tgcggtggct caagcctgta 117540atcccagcgc tctggaaggc cgaggcaggc agatcacgag gtcaggagtt caagaccagc 117600ctggccaata tggtgaaact ctgtctctac taaaaataca aaattagcca ggcatggtgg 117660cacatgcctg taatctcagc tactcgggtg gctgaggcag gagaattgct tcatcctggg 117720aggcaggggt tgcagtgagc caaattgcgc cactgcactc cagcctgggt gacagagcaa 117780gattccatct caaaaaaaaa atctgaagtt actgagaaac tggtatgaaa tcattaagaa 117840aactaaggtg tctaaattaa tacatacaaa gagattctac aacacggtat ttataaattt 117900aaacaaaatc catggccagg cacggtggct cacgtctgta gtcccagcac tttgtgaggc 117960caaggcagaa ggattacttt ggggccaaga gttcaagacc agcctgggca acatagtgag 118020accctgtctc tacaaaaaac aaacaaacaa acaaaaacac tgttttagcc tggcatggtg 118080gcacatgcct gtagtcccag ctactcagga ggctgaggca tgaggatcac ttgagcccag 118140gaattcaaag ttgtagtgag ctctgatcac accactgcac tctagctgta tcacttatga 118200cagagcaaga caatatctca aacaacaagc aaacaaaaca aaaaactaaa gtaaatccag 118260gtaaaataaa catacgaata aatgaaaaga tctgggttat tgcattagtt ctgtttctag 118320gcttaactac aaaatggtgt ccagttgtta aaaacacttg tttaagagta ttaataattt 118380ttttttttac tatgctcttc tacatcctag gaaattggca acactaagaa ttcctaaaaa 118440caaacaggag tgttaactgt atataaatta ttatttaaga tgcattcaag gcgttactta 118500aaatatcaaa tgcatgtcaa agtacaaaca acaaaatcat gcttcaacaa atgtgggatg 118560ttgccattaa tggacagtta gctctgaggt aaaaccaaga atccttcatt acctattttt 118620gtaaattagg acaaatacta ttaaggtatt aggttggtac aaaagtaatt gaacaaacct 118680ggacccactc acctcactga aagtgctgcc tggctacagt ttctagatat aggaatgtga 118740gaaatggtag cagccttgaa ggtttagagc tatattccca taccaattat ggctagaaac 118800ttaaaacagc tctattgaga cattatggga agtctaattt cctgttactc tactttaaca 118860cagccaagta aaacagtaag aaagcttgct taattcaaca aggactgagt gccagagtgt 118920cacctggaaa cttgtgacaa ataatcatgc ctcacacatt catccttggt ttttactacc 118980aaatgcaagc ttcatctatc ctcttccatt ctaaacttga ctcatcctcc taacctcttc 119040cagaaagatc tctagaaacc cagaggcttt ctgaagaccc ataggctatc ccccatcttc 119100aactacttta cataatggcc cctcaacttc ttagctttca tgaaagcctg gctcctcccc 119160aaaggcactg cctatcatgc aaccctttta tgtgactcct aaaactccta agtccaccat 119220tattcttcta attccctgta ttaaaaataa acggcaaaat tccctgtatt aaaaacaaca 119280aaaaaacagc ttgtaatttg aaactcatgt catctaaccg aatgccttct ctttccctcc 119340ctgttctggt caattaaacc cacatattta agcctccact ttgcccccag ttacatcatc 119400agcaaagtga cttcaatgtc cctgtacatg gttctatccc aatactctag gcccccaaaa 119460tatttgcctt tctaaatttg agacatcttc acctcctcat ttcagcaaat cactcccttg 119520tctttgttag acatactaaa acctctgaca atgatcatct cagctatctt tgtaagagtt 119580tcactcatac tcatatctcc tttcacctgt caagacacca ggtttggtga caaataaaaa 119640tgatacgtat ttgggaggct gaggcaggag aatcatttga acccaggaag cagaggatgc 119700agtggccaag atcatgctac tacactccag cctgggtgac aaagcaagac tctgtctcaa 119760aaaaaaaaaa aaaaaaaaaa aacacaaaaa acaaaaaaaa ggatacatat ttattgtatt 119820cacatgatgt tccaaaatat gtatacactg tggaatggct agatcaagct aattaataca 119880tgcaatacct cacatactta tattttatgg cagaacattt aaaatctact ctcagtgatt 119940tccaaaaata cactgttatt aactagtttc catgttgtac aatagatacc ttgaacaaat 120000tcctcttaat tgaaattttg tgtcctctga ccaacacctc tgcaacccag tccactcacc 120060cccagcccct ggtaaccacc attttacatc cctgagttaa gattccacat ttaagagaga 120120tgtagtattt gcctttctgt ggctggctta ctttcaacaa tgccctccag gttcatccct 120180gctgtcacaa atgacagcat ttcctccttt ttatggctga agtatattcc actgtgtata 120240cataccacat tttctttatc catgaacact taggctgatg ctgtatcatg ggtattgcaa 120300atgataccga aaggaacatg ggagtacaga tctcttcagc gcactgattt tattctttta 120360tttatatata tatatatata tatatatata tatatatata tatataaaaa tacatatata 120420tccaagtgtg actgctgaat catatggtag ttctattttt

taatttctga gcaaacgtca 120480tgctattttc cataaatgct gtactaattt acattcctgc catgcgcaat atatctcttg 120540tctgcaaatc ctcatcatac tttaatagcc attctaatgg ccatttgtat gtcttatttt 120600gagaaatgtc tattcaagtc ctttgcccat ttcttaagtg aggtgctttc ttactattga 120660gatgtctgag ttccttatat attttgcata ttaacccctt accagatata tggtttggaa 120720atactatctt ccattctgtg agttttttca ttctgtagat tttttctttt tgccatgcag 120780aagcttttta gtttgatgtg atctcattca tcaattttta tttttgttgc ctgtgcttcg 120840agggggtcat atccaaaaaa atcactgtcc aaaccaatgt catgtacatt tctcctatac 120900tttattctgg tagttctaca gtttcaggtc ttatatttaa gaccttaatc cattttaact 120960tgaatttggt atatgatgtg agatacaggt ctgatttcat tctcatgcat gtagatattc 121020cattttccca acaccaccta ttgaacagac ttccctttcc ccgctgtgtt ctttgcaccc 121080tgtccaaaat caactgatag tacacgaatt tatttctgtg ctctccattc tgttttgtgg 121140gtcgatgtgt ctgttttttt gtttcttttg tttttaaaag acagggtctc acgctgtcat 121200ccaggctgga gtgcagtgat gcaatcacag ttcactgcca ccttaagctc ctgagctcaa 121260gcaatcctcc ttcttcagcc tctcgggtag ctaggactac agatacacag caccgcgcct 121320ggctttaaaa attttttttt tttttaattt tagagacagg gtctcactat gttgcccagg 121380ctgctctcga actcctggtt tcaagtaatc cttctgcctc agtctcccag agtgtgggaa 121440tacacgtgtg agcaactgtg cctggcctgt ctacttctat gtgactatca tgctgttata 121500attactgtag ctttgaacta tattttgaaa tcagatagtg tgatgtctcc agctttgttc 121560ttttcactca tgacagcttt ggctatttag gatcttatgt ggttccatat aaattttagc 121620actgttttct agttctgtga aatttaacat tggaattttg atcaggattg cattaaaact 121680gcagattgct ttggataata aaaacatttt acaatattaa ttcttccaat ccataaacac 121740aagataactt tccattattt gtatcatctt caatttcttt aatcaatgtt acatagtttt 121800caactctttg gttaaagtta tttctaagta caatcatccc tcggtatctg caggatattg 121860gttccaggat ctcctgtaga taccaaaatc catggatgct caagtccatt atataaaatg 121920gtatagtatt tgcatacaac ctacacacat cctcgtgtat actttaaatc attacttagt 121980acctaataca acgtaaatgc tatgtaaata gttgctatac tgtagtttcc ttattgtata 122040agaaaacagt atttttattg ttgtattttt ttctcaaata ttttcatcca aatgttggtt 122100caatttgcaa atgcagaacc actgatactg ggagccactg catgttttgt ttttggtacc 122160tattatacat ggaattgtct tcttgatttt tcttttagat aatttgtcat taatgtacag 122220aaattctacg gatttttgta tcttgatttt atatcttaca actttactga atttatcagt 122280tctaacaatt ctctggtgga gtctttaggt ttttctatat acaagatcat gtcatcttca 122340aacagggaca acaatgtctt cctttcctac tgggatgcct tttctttctc ttgcctaatt 122400gctcttgcaa ggacttccgg tactgtgctg aatagaaatg gcaaaagtgg gcattctcgt 122460tttcttcctg atctcaaaag aaaagctttt aacttttcaa cattcagtat aacgttagca 122520gtgtgtttgt caatatggcc tttattttat agaggtacat tccttctata cttaattttt 122580taaaattttg tagcacaaga gaatactgaa ttctgtcaaa tgctttttct caattatctc 122640aattatctat tgaaatgata acatggtttt ggcctttgtt ctcctaatgt tgtatatcac 122700attttatgga ttcaggtctg ttggataatc cttgtaccct tgaggtaaat cccatatgat 122760cacagtaaat gatccttcta atacactgtt gaatttagtt attattttct tcagaatttc 122820tgcatctaaa ttaagaatac gggtctgtaa ttttcttttc ctggagagtt cttgtctggc 122880tttggtatct aggtaatgct catctcccaa aatgagtttg aaattactcc ctcttcttaa 122940atttctcata aaattatgag aacaatctgt actagttctt ctttaaatgt ctgctagaat 123000tcagcagtaa agtcaccaag tcttgggctt cctttggtgg gagatatttt tatgactgat 123060acaatccttg tcactggact gttcacattt ttcatttcat gattaagctt ggacaagttg 123120catgtgtcta ggaatttatc catttcttct atggtaatca atttgttggc atataattgt 123180tcagtctctt atgatcctct tatttcttgt acttctctgg tatcagctgc aatggttcct 123240ctttcatttc tgatttaact tgagtcatct ctttttttct tagtctagct aaagacttgt 123300tgcttttatc atcaaaaaac acaactcttt atttttttta tcttgagtca tctctttttt 123360ccttagtcta gccaaataac ctgtcttttg agttcagatt cttttgtctg cttgctcaat 123420tctgctttta atgctctttt gcatttttca ttccatttat tgcattttca gcttcagaat 123480ttgttaaaaa ataatttcaa tctcaaaatt tctcattttg attgctgttt tcctgatttt 123540gttgaattgt ttatctgcat tttcttgaaa ttcattgagc ttccttaaac aattactttg 123600aattctttgt caggcagttc atatatctcc atttctttat ggttggttca ggtgccttat 123660tgtgttcctt tgactggtgt cacgtttccc tgactgttct tgatccctgt agtcatgtgt 123720tggcacctgc acatttgaag aagctgagac ttgttccagt ctttgcagag tgactttatt 123780gggtaaagcc cttcaccagt cagcctgtcc agagattatg cagcctgagt ctaccggggc 123840tggcctggag tctgggtcag cagggtaggt cctggagcct tggtacacag gtgctgctgg 123900cctggcatcc tggtctacta gagtgagcct gaaccttgga tctgcaagga ccagtgtgaa 123960ggctgggtag gtggatgttg gcctagaatt tggatcacaa ggggctggcc aagtactggg 124020tttggaccct tgttctgcag tgatgtgagg ccacaggggc cgatctagag ggcagggctg 124080cagggaccac cctagcacta gacaggctta gagcctaggg ctgtgggatc cagcttggtg 124140ctggggtggg cctccaagct tggtttgtgg gtattggcct gaagtttggg gccatggggg 124200cctgcctagc attgagtttt actggggcga gctcaatgtt agggtctaag tctggtgtac 124260acttcccctc cttcccccag gtgaacagtc tctctctcca tgctgggatc tcagggactg 124320agggaggtgt gacacaagta atgtaaaact gtccttctta cccttttcaa tgtgtctttt 124380cttatctgtg tgccacattc aggtaatctc tcgtctggtt tcttagctct tatatttttg 124440tgtgtagaca gttgttcaaa ttgatggttc tacaatgaga tgagtgctgc aaagtgctag 124500tctgccatct tgctgttatc tagacctcag gcttttctta aaacaactca acactttggt 124560ctggcatccc aacctttcca atcatggcca tcatccacag catataaatt caaatactcc 124620aatgttaaca tcttatgcac atgccactaa tgattctgtt ccatcagctc tgctaatatc 124680caaccctaaa ataccatgca taatcttctg gctattggag aaaagtcttt aaatttcaaa 124740tgtgataaag tctttaatct taactagtca ctcagtggaa tgagattctc ctactctgat 124800acggtttctg atttcaggta gccagccagt caagactctc aacttctgat ccacttcttc 124860acccattccc ctcagtagct tcctaagttt tcagtcttct caaaacctct aaacaaacct 124920tgtattccac aaaatattat taaaaaatgt ttcataaaaa gtttcgtggt cactaaccta 124980cagtaacata ctaccttata tccactacaa tgactactat tgaagtacac acacacacac 125040actctcacac aatatgtgat ggcaaggatg tagagaaact ggaccccttg tgcactagtg 125100gtagaaacgc aaataggtac agttgctctg gaagagtatg gcagtttctc aaaattttaa 125160aaataaaatt accatatgat ccaacaattc cacttctggg aaaacaattc aaagcagggt 125220ctcaaaaaga tatttgtaca ctcctgttca tagcaacatt atctgtaata gctacaacat 125280ggaagcaacc tgagcgctca tcaacaggta aatggataag caaaatgtgg tatatacata 125340caataaaaca ttattcagcc tgaaaaagga tgcaaatttt gaaacatgct aaagatggat 125400gaaacttgat gacactgcga agtaaaataa gccaatcata aaagggcaaa aactgtatga 125460ttgcacttat agggggtact tagagcagtc aaaattagac acaaagtaga ataatggttg 125520tcagaggctg tgaagataag gaaatgagga attattgttt aatgggtaca gagtttcagt 125580tctgcaaaac agagagttct gatcgtgact gtgcaacaat attaatatac ttaataccat 125640tgagccaaat acttaaaaat ggtgaaatgg aaaattttac attatgtgta ttttactaca 125700attttttaaa gcttaaaaga caagtcacat ggttgggtca cataaatttg agaaatacat 125760tttatagtcg tttcttgcag attcacaata tataccggca tttaaataag gttccaatat 125820tttcagtaaa aaaatcttga tttttccacc ctcacccaca attacaatta atatcacaca 125880tccatcttta cttccttgtt ctaatttcat tagatgggca tctttcctct tattcaaggt 125940caatcactat atctgcattt ttcttttttt atattacctg tatgagagat acacctgtat 126000ttttcattcc aacccttaat acctcgaaaa actttgcttt gataattact acctctatta 126060tctgaaacat ctttagtctt cacaataaat cactaaagtc ttacttactt catgtcctaa 126120atatttgtct taaatccaga aaaatttatt ttgaattcct attataccac tgtcattgtc 126180aaggctctca ctgactgaac taccttacca ccacacgttt ctcttcaatc ctttctctct 126240tgcaaggtta ttaaaaatac acgtttgaca aacttactat ctacctactg agcaaattct 126300ttgctgactc cctaatacct tctatcacat cttatcttct tcattttcct acctctcagc 126360tagtttatga tctaaaagtc ctcaactgcc tattgtcccc tactccctat gctgcttcta 126420actttggctc atacttctgc ctggaataag ccgctcatgc ttctagctgc ctgattccca 126480gtcacatttc aagactgaac ttagcatttc ctggccactc ttacttccct gccatctaca 126540ctagattcct attgcatacc tgggtactcc agtactcctt catgttacta ttgtattttg 126600gataccattc tacctcagtg actgaaatta tttgcttctg catctttcac tcttgagtat 126660aatataaatt tctgtagggt gaaaactaat tattacgcat ctctgtatct ataacatagt 126720acttgatgcc ttaaatatgg taggcgttga gactgttaaa ctcaactctt tcctcaacct 126780ccctatttcc ctacaactgc taccagggaa gcaaaaagac taagaattta ggcgtgtgca 126840tttatctatc tagtagtaga aacctagtaa actgttaccg aggtttagag ttatctgata 126900ggaaaaaaag taggtatgtt cctatctccc atttactgct aaatgattcc tttcttttaa 126960ccagaaatta tttccaaata atgtgacgta atttaaagca gtctagggca actcaaaata 127020agaaccacag tctcaaccaa gttacactta cattcggcta gcttcaatgt cgtcagactg 127080aggttctcct gatctgtaaa atgcaattga gaaacagtta gcaaaatatt ccatattcca 127140aaaaaaaagg tagacatatt acttaggaag acaattttgt aaacaagatt taagcactaa 127200ataaaacagt tttaaaatta aagatgtcaa catgaaagaa agtggtcaga tcaggtggcc 127260atacaacaca tttattttaa gggttggact attaaaaaac tcaatgcatg ttttttaaag 127320gaaactgtaa acgctataca tagataccaa cagacaagcc attataagcc ataattattc 127380acaaagtcaa atacgtgtaa taaaacacac aactaaaaaa cttcctatag ccaagtattg 127440gttatccaca ttaacaaagg tactcactgg tattcactca cagtattaaa ctttagatta 127500gtggctcagt aaactactcc ctaggcagac agactaaagc ttaggccagc catgtagttt 127560tgtaaatgaa gttttattgg gatgcagcca tactcattca tttaggtatc atctatggct 127620gctttcacac taaaagaaca gagctgagga cttgtggcag aaaccacatg gcccataaag 127680cctaaaatat ttattttctg gccctttaag aacaaatgtg ccaaccgcta ccgctactcc 127740agacaattca aactaaaatc taataccttg gtaagtttaa agacacttaa atcagataca 127800acattctaat gatgattatc ttggttcaac aatgaaactg tttccctaaa aatacactta 127860ctgtatgtat tacctgtcaa aagtgtatca cctgaggtga agttttgcac tggatagctg 127920tgaattaact tcagttttaa acagttgcca gttactttgt tatttctaaa tatactactg 127980ctctctcatg tagaaatatt taaaattcta agtcactaaa aagcctttca ttttcatttc 128040acgagatatg aatcagtatt tccctatcta ttcactttta taaacagcat cagcaacaat 128100gaatgagtca atataatatt gatttctaac acttagaaat tcccaaacta gataaaaaat 128160gttttttgag gtcaaaagct aaattttcaa attggcatta caaagtcctg atttgtgaac 128220ttctcactga ctttaggctt ccaactttaa gcaactctaa caagaatctt aaaatcagtc 128280acacacacac acacacacac acacacgcac acacacaaac ctgttttcta aatttcttag 128340ctaaaaacaa aaacaaaaac aaaaaacaac ccctcacttc tttatcagac tctcaaaatt 128400accctcactg caattcattc agattccaag gacaagaatg gaaaaggaaa aaaaaaaaaa 128460aaaaagactc cagcagctca aatatttatt ttagatcttc tttgcaatgt atttaaattt 128520tcaattttgc acaaaattac ataacaacta aaaactgggg catgctggag gtggcagtga 128580cttggctgtg acacaaatgc aaaaatttca ctccaccctc tcccccaggg agcaagcaaa 128640tcacctatgt gacacacctg gtctccacca gccttgtggg taagtacccc aagattgctt 128700ccaaacttgt atctcccaga cagaagagct aaaaattgat gataacccca tgtaccagct 128760gactcatttt tgaaagtgcg accctccagc atcagatgtc atactgtaag gaggcagagc 128820tggcttgcaa aacaaaaaat aaacctgctg gcactgaggg aagagaggca gattagaagt 128880tttcaaacac gtaggcagca aaactttcaa aattaacagg gaaagagaag agagggaaaa 128940aatgaggaca tgatgatagg aaaataaaaa tattactgca aaaaggtaag acacaagaag 129000aaaaacaaat ctaagtctta gaagtacaat atgtaactgt tacaaagttt tgagatcctg 129060aaatgggata gagtttaaat acattatcaa atgataacta catctatctt cccaatttct 129120gttccctaag caagagaata tgtgaacttt ggcatcaaaa tgtttgccac caaactgtaa 129180catattaatt actacttttt acatatatta aagtaggcta ttaaaaacca ttgataagac 129240aagctaagta atttaaacca atccagtata gaagaagaag gattgaaaga ttattacctt 129300aaaataaatc tgtcctgggt ctattaaaca tagcaaagtt gttctactca aattggaaat 129360tgttttttaa tcaagagaaa aatgaatata tgcagaacta gcagacattt ttcttgcagt 129420aatttataat agtttctgtc tgaatgaatt tctactcatc ctaataacag tatatttcta 129480gaatattata aataagttac caaagtcccg aggttagaat tcaaactctc aaaactatga 129540catatgccag gtgtggtagt tcatgtctgt aatctcagca ctttgggagg tcaagacata 129600aggaccactt gagctcagga gttcaaggtc aacctgggca acatgatgaa acctcgtctc 129660ttagccgggt gtggtggtgg gtgcctttag tcccagcaac ttgggaggct gaggtgggag 129720ggtcacttga gcccagaagg tcaaagttgc agtgaactgt gatcgtgcca ctgcactcca 129780gcctgggcga tggagtgaga ccttgtcttg gaaacaaaca taaactatga aatatatacc 129840acctagcaac agttcccaag cttgactttg gctcctaaag agtctactta ttcttttact 129900gccaagttgc tggaagtaag aatcctgtat gtgcttgtaa atgcagaaca tataacctaa 129960ttcaaaacat ataaccaaag ggaaattttt gtattaaact attgacttag gttatcctaa 130020agtgtgtcag aataacaagt cagaaatttt aaaactgaaa caataaccaa ataacattgg 130080ataataaaat gtaaatctca gaatgagaat caattttagg tcttgatttg aatcgcagaa 130140aatatgatca caaaacaccc actaatctga atactgcagc atgagctagc aaattcaaat 130200ggtggctcac ttccaataac actggtgcag cttctccatc ctgcaagcca ctccttttac 130260ctccactgta actctctagg agagaaaagt atactagttt tattaatgtt ataaaaactt 130320ctagagaaat gtttagttaa aacgttcacc acataaaagg catactttaa aatgcatatt 130380aagaaatttt taatgttttc tataatgttt atagtaaaat gtttaaaacc ttgaagtttt 130440tttgagaaat actttcctgt ttatacatac agataggtct ttgatagtgg ctactgatat 130500ttttaatgag aaaaaaagac actaaaatat tttaaggaga ttaaatgaag taggtcaact 130560ctcaggccca ctattcttag cagtattcct gccaactata cattgtcttc atttttacag 130620tatgtatttc actttacaat gacagccttt taaaggctgt aaaataattc aaaattacct 130680tttacaagct gtggccattc ggtgacatca gggtgatcac agctttgagt cactgattaa 130740aaacaggttg tcacaccagt ctagctgcta ccttgatctg agcgtaggct taataactct 130800aataaattaa acaaaccttt ggttagaaca ctttaatata taaacccaat tcattgttca 130860aaaaataata ttcaaagctt caaattaggt gtcaatgtaa aattcatcag aagagtaatt 130920attatagaaa agtttttaag gactattttc cagcattttg tttttcttaa atgatctctt 130980catttgagaa aatgtaataa ttccttgtac ttttatgtaa tgttatagtt tctgaagcat 131040tttggtcaat agtaccttga gtcttataac aactttggct ttacctaatc aagacagata 131100atatactaag aattaaaaaa aaaaaaaatt aagcagcctc cgagtgatta actgacttgc 131160ccaagatttt ggaactagtg ttgaaggact gactaaaacc cagatatact gacttcctat 131220caagggatgg atttttttgt ttgttttggc ttagttttgc tatactgggt ttcaagattt 131280aagtatatgt aaatgagtaa agattcagaa cacagattga gatctttcaa atacggacaa 131340attcagtcaa aagattccta agctgataga gcaatcagtc accaaggagg gcaactgctt 131400aatggaatag ttatatacat acacataaac acacacacat aaacacaaag tctagtccca 131460ctaattctgt atgtacgtaa agtcatttaa catcagtcac cgcatgttct cactcacagg 131520tgggaactga acaatgagaa cacatggaca caggaagggg aacatcacac tctggggact 131580gttgtggggt ggggggaagg ggagggatag cattaggaaa tatacctaat gctaaatgac 131640gagttaatgg gtgcagcaca ccagcatggc acatgtatac atatgtaact aacctgcaca 131700ttgtgcacat gtaccctaaa acttaaagta taataataat aaaaaacatc agtcaaaacc 131760ccttaagtac tgcataaaaa tatattctac tctttcttgg ccataaatct actaagattt 131820aatttcatga aaaagtatgg caaaagatga aagcatatcc tcctccctca accaaatatc 131880tttttaaccc cttacatggc cttagctgaa cttttctgat tgtcacagtt caaatttagc 131940aggcctcttt actgacactg tagttctggc aaagatacca tacatctgaa atatcccagt 132000atcaaaagat cattttgtgc aactagttct ttcccctcac gattccccta tccaggccaa 132060atctgaggca tcaactacgg aaagatgagg aaattaggag gaagaaacct ttcataatta 132120acatcctcaa agcaacaata gtcaagcgta aaaggtggtt accagtgtgg attcagaacc 132180caatataaaa cagcaaggta tctgcgaata ccaacggtta aaacatgaat gtttgagccc 132240atacctatgc agctatgaac gcagggcttc aaaatccaga ttaggtgagg attggcctga 132300atatataaaa ataattgcag gggattccaa taaatgggat ttatagtatc tgtaagaaat 132360aacgcaatct cccagatact cctatcctaa ctatacattg cctaataaga acttagacat 132420tgctgaggtc atatgggaaa caccaccttc cctaaaataa acaatgtggt catgatacag 132480cttccccttg ggttctcaag agactaatgg tatgtggaat tagaagattc aaacaagtct 132540aactttccat cttgtaaaca caaaaataat aaagttaaaa tatcttttaa ttgactaatt 132600gacctcaatg taattaaaga gctatatgta ttttgaaaac tagagtcaag tcctctcttc 132660cagtcccctc ttttatgaga tccggtgctc aaaaggctcc aaggccaggc tggacttatg 132720tctctgtttg gtcattcact agctgagaaa tcttgggcaa gttacttaac ctctccgagt 132780ctcagtttcc ttataggtag aactaggata atagtaccta ttccatagag ctgtcatgga 132840taaacaagct aacatctaag tatctcagaa cagtgcctga cataatgtgc tcacaaatgg 132900ctgggcgtgg tggctaacgc ctgtaatccc agcactttgg gaggccaagg cgggtggatc 132960acttgaggcc aggagttcga gactagcctg gccaacacgg tgaaaccccg tctctactaa 133020aaatacaaaa cttagccagg tgtggtggca cacgcctgta atcccagcta cctgggaggc 133080caaggcatga gaatcacttg aacctgggag atggacgttg cagtgagtca agaaggtgcc 133140actgcactcc agcctgggca acagagcaag actctgtctc aaaaacaaca gcagcagcag 133200cagcagcaac aacaacaaca aatgaaagct attattaata ctcatagccc aaaatccaaa 133260cctgccctct caatcttttt atgcatatta ttttacctct catagcacac taatctcttg 133320taaatttttt cagcacccta cctgggtccc tcatacagtc ttggaagtgt agttagtgag 133380cacctgacac aatgctttaa aaatggcagg ggcccaaatt tgctgaacac atctgtcatt 133440attatgacat taagatacac atgcaatacg aattccaaaa aataaaacaa aaacatacca 133500tatttcgcca aacttctgag gagctggtga attctaaaat caggctctta cagattttta 133560acctaaaata taaaacacct cactcaattc tttttacata aacaactgga tgaactttca 133620aaaaaaaagg tactaaattt catcataaag gcaaatttat tttctataat tatgaacatc 133680caaaaacgta tcaagaaaca aattactgct gttgccggga tgaggaaagc ataggtaggc 133740aggccctgtg gcctgagtgc tttcagcagg caaagccata tttcccatat tcatccataa 133800agcttagtgc cttctattta tttactctat cctccctcca aaagggctaa acacatatga 133860aaactatttg actatatccc tctaatcctt tcaaatacct atagcaattt tgtttttaat 133920taaattctat atgctaagca tcggctatgt taggtacaaa tctgggcatc taccactttc 133980tagagtagca gccttgggcc gaccctctga cccttgagtc tccaatgtca ggttattgta 134040gcagagaaaa tgcgttaaaa agttatagta taatgcctac ctggcacaaa acaggacttc 134100aatatacgac gataattata atttattaaa tcaaccactt aatatatatc aaaacttaac 134160tcttaaaaaa aaatcctgaa gcttaaagca agcccctaaa ttatcactgt aaattcttaa 134220cttgtttgta tacaatcaga acattcctca atggaacaaa aataaagtcc tttcatgtac 134280tgcattacat ataaagaaag caaatctcaa aggttaagaa acctatccag tgtcctagag 134340gttgatggta gaaagtgaat acactgcata taaaacattt tgaaataaaa ttaaccaact 134400tttaaggttt tagagactga ctttcaaact tcagtctata atagaattac ctggggaaat 134460ctaaaaatgc aactacttgg gctctactcg caaagatttt cattcagtgc atgaagtagg 134520ggtaaatgaa tttttttttt aatttttttc agaccaaccc aagaacgcag aaatgaattt 134580gcattttaac atatacttca catgacacac agatctcatt ttaagaagcc ctgccttcca 134640aattatgagt taggaacaca taatcatgga ggaaatatac caaatctata aaaatacaga 134700ataacaaaaa ctgtaagcac tacaaatatt aggcacactc acttgccaat ttaaccataa 134760gaatcctaat accagacata gttctcttac tttatctctt caacagaggg ctcagtctaa 134820taatcttata aataagtata gtctaataat aaatgcctat gttgatctat atctacctcc 134880ctaaaatttt cattttattc atttctattt ttccttcacc tccttctaaa gctgtttcaa 134940aacttaattt cacaaactgt ttttttctat cactttttat tataacaccc aacacttctg 135000ttctcctttg catcatgttt atggtgtctt atatttatgg ataatgctac ttattaaaac 135060tgtctaagtt ttgtccgttt acacatttca caattaaagg actactacaa caattttcct 135120aagtcctcct gctttagttt ctccttattc cagtccattc tgcagttaag acttaaaaag 135180ttttaaatac tgctttcaaa taatacttgc aacataattc cctcaactcc attagctcct 135240cagagtacca tttctttatc ttctttcctt ttactgccaa aaagacgtga aaaccactag 135300ctcagctgct atttttctat ctactgaaat ttttcttgta aagtttccat catttctgta 135360tattcccagt gctttggccc cttaatccta cttaattttt gtagctttta agactgctaa 135420tgactcctca cctcctctgg cttccctaat gttgtatcca ctcattttct tccatctctc 135480taaaggcaat aaggtcttct gctagttcat cttcctacct

catacatggc ctctctccaa 135540gtttctgtca atagccactt ttcttaacct aaaccaattt tctgcgcaat gtcatccatt 135600cccatagcac ctctatgctt acaaacccct aactctgtat ctttaagtcc ttatgtctgg 135660catggtatgc ctgcaactac atattaccaa cggtacacat ccatttggat gtctcattaa 135720catctacatt caacgtatct aatatcaaac tcaattattt tcccaaagaa ctcactctgc 135780ctaatctcac tgtcctggtt aaggactaaa aaaattggtg ccattcatta ctttctcccc 135840ctcattttta tttgccctaa ccactgtgta ggttgatact tgttcagcct tcaaattctt 135900cataatcatt aattcaaaaa ctgtctacta gattttgagt gcctactagg tggatgaaga 135960tgcagaggta gaaaaactag acataatttc ttgccctcat gtggtgaagg aaatgggcac 136020aaataaaaac cacaattaat tatgcaataa caatgcacag ttaagtgttg tgatggagag 136080atacaggggg tgttaagttg tgtactgtaa taaaaagtcc ttagtctgaa gaaaaggaaa 136140accattcttt atgagggaca agcaagattt acaagacaca ctaagaaaaa ccctgtgagg 136200taatgatggt agcactatca gtccagctgc tcaaattgga agattctcag tcatattcta 136260aagagtctct ctctttccca gtatatcaaa ttgtttacca attgctacca attcatcctt 136320agaaattccc ctacatttct acattctcac attattcccc agtctttttt gattgctttt 136380agttgcttcc agaataaagc caaagctccc aatcacgtca gatgagattc tacacaattt 136440ggtcaaaacc tgccagtgca gcctcttctt acagactaga ccagacttca agagaacatg 136500tgtatcattt gcttttatac ttccagtgct tgcaaatgtt tatagaataa gcatcttaca 136560aaagagaaaa tttttctctt agggaactag ctttcagtcc tttagtaaat gcaaggttaa 136620gattcaaacc ctaatttgtc tgattctaaa gaccacgctt attcccttgt acttgccttc 136680taatttatac tcaaacccta tgaatttcct taaaaatatc tcttactcct tttttcctca 136740aacttaccaa cactgtccta ctacagtgcc ccattatttc atctggtttc ccttttgcct 136800ttatcctcaa ctttgccact agaattatct tcctaaaaac tcaaagttac acatatcatt 136860ctatattcaa aagctttaac actttctcat gccaacagaa taaatttcta actccttcac 136920attttatttg aggcccctgc catgtggccc tgaccccagc catcccagca ctactgccca 136980ctatatgaca tcatcaccag ttaccagatc tagtcccatc atttgactat tggctataat 137040gtactggata tttgtgtttc tgacttcaat gcttttgctt ctgaggagac ttcactcgga 137100aatggtcctc tatccattac taacctctat cccccaccca cctatcatgc catgttaaaa 137160actggcctca ttctaaacac tgtatctcac agggttatct aaactcttat acataatcac 137220catctccttc atgtcttatt atacattctt aataccataa aataatttat taatttctcc 137280ttatatttat tatgttatac cttacattac agctatctgt atacatttgt cagtgcctct 137340agttgtaagc tgattgatgg tagggatcat ctcttccata ttttactttt cgtaataatg 137400gtgactatgt tttcttgaca ccatacctcc cctctacaag aaaaaaaaaa aaaaaaaaaa 137460ggaatcagga cccatggcct ttgagctagt tccaaatgtg actcatagat gaacaaagat 137520accatttctt cccaaattaa cacaaatata agtaaatgcc aatttaagaa acgttttttc 137580aaagactaaa tatgtaagaa taaccaaaac aattgtttct aataatgttc ctttctctac 137640cagatattaa aacatattaa cttaaatgga ataattaaaa tcggcatgcc tttaaaaaac 137700tgagtataca ctaaatatat gcatacctta tgacttaaaa atttcactct taagtatacc 137760aaacagaagt aaacatattc acaagaagac aatcactgaa atgtttagag cagcagcaat 137820attcttaaca gttccaaacc aaaaatgatt aaatgtccgt aacagcagat taaactatgg 137880catattctta aaatggaata tcacttccat tcttaaaatg gaatatacag cgactatcat 137940aaacaattac tacaaccaac aatgtgaata aatctcacaa atacattaaa agaagtcaga 138000cacaactaaa tgtgcttgat tctagttaaa ggatgaaaac aggcaaatct aatctatgct 138060atgagaagtc aggacagtgg ttacacatgg aagaaaaggg gtagtgacac agagcacaag 138120gggctacgca ggagctgctg ctgttttttg atctgaatgc tagctaaacg ggtgctcagt 138180gtgtgaaagt gcaacaagct atacacttag aagtgtatag cttaaaacat tctaaaaaaa 138240aaaaaaaaaa cctcatatgg tattagcatt agacaaagag gtaagcacaa caagcacaac 138300agaaagctaa tttcagagaa agatccgaaa gtatctgaca gttgctcctt ctcaaacgag 138360aattttcaga ccttggataa caggcagcag aggactataa tgcatgaaag gacaaaaaca 138420aaggaggtga gccatccact gcttggatgt ctgcctggag gtaatttcca acctgcagtc 138480aatggagggg aaatccagtg agaagacagc agttttactg agttcagaat caagagatca 138540gagctgagaa agctgaagtg ggtaaaattg atgacaagtg ccagacaagt gcttgtctga 138600gaaggcagag aaatggcttc agaaatctgc catttgagag tccactgctg agtactaact 138660gtacacgatt aaggtaagtg actccatgaa gctagtgaaa gaggggcagt aagctgaaat 138720attcccagta cttataaagg actgggagac tcctaaaatt ctctctaacc tgagtgaaga 138780gaacacggtg acactgggag catttagtaa agactcagaa ggatcatgcc ttaggtagga 138840gctactacca ttaaagcaaa gccttctcta gacaaactcc aatgtagccc tgaagggata 138900aaactgacca gcaagttatt taggtggcta caaaacaaaa cactctaaaa gaaaacaaaa 138960tctagacacc caacaacaaa atattacaat gtccagcatc caatcttaaa aaatcattaa 139020atatgtaaaa aacaaaaaat aaaataaaat aaatactagg aaaatacaac ccatgaccaa 139080gagaaaaaac caaccaacaa attcagagtg aaaaaattca agaaatcaat aagtaacctg 139140attttcaaac agctattaca aatatgctca agaattcaaa aaaatacatg aataaaatga 139200atcaaatgga atttcttgaa atataaagcg aaatatctga aattatttaa caagggagga 139260gaaaaaacag taaaatagaa gacaaagcaa actgaagcac agggaaataa aaagactgaa 139320aaataatgaa tatgatgtta atgacctgtg gataatacca aacagtctaa catatgtgta 139380actggagtcc cagaaaaaga ggaaatgatg aaagaggagt aaaggtggta aaaaatatat 139440atatagaaga aacaatggca acaatcttcc aaatttaagg aaaaccataa atgtacagat 139500tcaaaaagaa aaaaaaaaca aaaccccaac aactctcagc atggtaatca ttgtgcaaat 139560atcatagaac gtacttacac aaatctagat tagatggtat agcttactac agaactagga 139620tacatggtat agcccgggtg ctcctaggtt ataaagctgt acagcatgtt actatactga 139680atgaatactg taagcaactg taacaaagtg taagtattta tacatctaaa cctagaaaag 139740gtacagtgaa aatatggtgg tataatctta aatgactatt gtcaactatg atctcgttga 139800ctgaaatgtc agcatgcggc acatgactgt ataaccagac ttctcatcaa aaacaaatgc 139860tgaaagaaaa acacatcaac taaccaagaa aaaagaaaca tatatgtcca cacagagact 139920tcttttttta cacaaatgtt cattgtacct ttttataata gctgggaact gaaaacagcc 139980tggaactgaa aacagcccag gtgtccctta acagatgaag tcacaaacca ccgaaacatt 140040caacaatatg gatgaatctc aaaaacatta tgttgagcaa aaagaaactt tttacaacat 140100ataagtattg tatgactcta cttacataaa gttctgcgca agctaaacta agatgaaaac 140160aatctgaaca atgttaggtt ctaggggtgg tgtgggggga aggaataact aggaagaggt 140220gtaggttcta taccctgata gaactttctg gcaaaggtgt atacatttgt cacagctcat 140280gaaatggtat atgtggtcaa ctgtattttc caaagatggt cacaatagaa gccattccac 140340atgctcttaa caatgtaaca ctgtcatttt ttcatcaggt ggtggagtct atggcccttc 140400tccttgaatc tgagtgaaat tagtgagtct ttcaatccaa agactacagt catacctcaa 140460ctatctacag aggactggtt ccaggactcc catggatccc aaaatccaag gatgcttcga 140520atcccttata taaaatggca tggtatttgc atataaccta catgcatctt caaatatgtt 140580aaaccatctc tagattactt ataataccaa atacaatgta atgctatgta aacagttgtt 140640acaatgtatt gtcaaagaca agctctcctt gccaatccct gaccaaatga gagattcatg 140700agcaaaataa atagttgctg atatttaagg cactaagttt ggggcaatgt attacacagc 140760aataaataac tggaacagca tagtaaattc tagttaaaaa tactaaacta ctaagtttta 140820gttaacatac atgctcaagt taagggtgtg gtatactaac aactgcaact tactttgaaa 140880agcataaaaa aagacactag aagaagacag gatggataga tggacaagat agaagacaat 140940aacaaataga acaaaatgtt gtgaaattat attctacaat tattttaaag tctgtgtggt 141000ttgaaaaatt gtacgataaa atgaagagtg aaataaagac atttccatac aaa 1410532150237DNAMacaca mulattamisc_feature(64387)..(64600)n is a, c, g, or tmisc_feature(76284)..(76747)n is a, c, g, or tmisc_feature(134705)..(135171)n is a, c, g, or tmisc_feature(145964)..(145983)n is a, c, g, or t 2cagatgtgca gccctccaat aggacaaata agactctcag agcctggctc tatttgggga 60tccctcagtg acaacagagt acccctgtga gcatgccttt tctatctctc ctgagagggc 120agtagcatgc tgtcttagga gtcagtatgc tctttagtat gcttagtcac ccaagacttg 180ctttaattgt agatagatac ttacatatag gaaagatgtt cttaagtaac aaatgaaaaa 240ctttagaaga ttgaattaag ggtcaagcaa ctgtgatatg tctgcaaatc tcattagtgt 300tatgctgaaa gaaggaaata tggcatgcct ttattaaata atgacaatgg aaccacgttt 360cgtgctttgt tatttttact gtggagtatt ttctaagatt atgtttgctt ttttttctct 420ttcatgtttt gctgagatag aaggcctgga gtctgatcct ccacttcaga gaataggggt 480gagtagctaa accattatct tttgaaattc atatgtcatg tgctttttgc taggtcttta 540ggttgttgtg tatatctttt cagaagctta ttgaaggaca ttttgatgat acgtcctttt 600cctcattgag accctcacca tgtcacctac actattgaat ctatcatttc tgttttaatt 660ttaactctct tctgctttta tggaaaaatg tagaatttaa gagaattttt ggcaatttca 720tattggatca aaatttattg tagtgaaatc caggtgtacc aaaatattaa cagagtttcc 780tcatctgttt aattattggg gtttcagaat agagactcca tggttcataa gatctttgtg 840gtcatactac attatatttc tgcttctaat tgaattatta agtattgact tgaattagtc 900tttttctcgt tgttgcagca aggtaagtta tataggaaat tttcttctct cgatggcatg 960cctgagataa tcatagatat aagacacctg ggtggtttct agtatgcgtg taatttttgt 1020ttcttgatct gtgtgttgag tacttgctgt gtttgcatca tgcaaataca ttgagcttta 1080caattttagt ggatgcactt ttctacatgt atattattct tcgatagaaa gtaaaaaaaa 1140acctcatcaa actagtcaaa atattggtta atacataaaa aagtctcaag tagattgtgt 1200attacatggt gcttattgat cgatgccctc ataatagatc aagtacgttc tctctttagc 1260ataggccttt ttagcaaatc gtgtcgtgag tagttactca agtattttta ttttaacaca 1320tttatgtttt ttctatgcat attcttaaat tctcttacac ttttttctct gttataaaaa 1380catgctgaac aatctcaagt cttaaagatt gcagtattgt ccctacatat tcatgtattt 1440tggtactcaa ttctttatac tttctttgac agatcacttg aacatgtctc ttgttttgca 1500gagagggaat tgatgggata ccttcatgct tttctattct atgtgctaca taattgaata 1560tacaagcaaa tatagttatt aaggtttagt atgattattt ccacaacaca tgcaaagaag 1620tttctcatac atcttaatag aggcccacat gcattgtacg gttgagaatt tggggaaatg 1680ttgatgaagt tgggtaaagt ataaatccaa aagtcagaac ggtgaactcc ttgcttaagg 1740atctcctttg agattactta gtcaatacac aactgataaa tttaagtgct tttcaccttt 1800tgagctctcg acacactaaa gctaaaatgt gtttcaactt ttaatcctgc ttccctaatt 1860ttcccttttt tagtttaaga tcaaagagct ccagccataa attactacca agagtaacca 1920cttcatttta agaaagctta gcaatataga agaatataaa attatttatg acagatgtat 1980tttaaccttt tccccatgct ttccagagga aatatgttta atcatctgcc ctatattagg 2040gaaaaaattt ctatgctaat acaagtatct atcaatcatt aatctttcta tataagatat 2100attgatcata accaattaat tttattgtaa atgagcttta gatttgacat tttggtagta 2160atatatgttg tacaatctcc tgaggtccta taggtcttga gatctctatg tcaaaaacta 2220tagatgtgtc agtgtttctg gtgaatgtga aggacgccac attttcctta gccatttctt 2280ctgttcagaa taatggttat gaacattttg ctactgcaag atatccatat atttataatc 2340agaatatgcc agtttttatg cactcatgcc tctgtttctg tagagcgttc ctagaatgag 2400taatgcttga aaattaggtc catgtggttt ctttaatgag atatagtcaa atcatgaaat 2460atttaggtta ccattatttc agtaatgtat tagaatgtca aggtaaagtt atctacattg 2520tatatataca cacaacatag atataattta aacaacacat acatttatat ttatacagaa 2580gatatatatt tatacaacat aaaatatatt ttattattta aacataaaat atattttatt 2640taatgtagat tctttttttt ttagtttaag actttgaagg ttaaaaaaaa atttttttat 2700tattatactt taagttctag ggtacatgtg cacaacatgc aggtttgtta catatgtata 2760catgtgccat gttggtgtgc tgcacccatc aactcgtcag cacccatcaa ctcatcattt 2820acatcaggta taactcccaa tgccatccct cccctctccc ctccccacaa taggccctgg 2880tgtgtaatgt tccccttccc gtgtccaagt gatctcattg ttcagtttaa aattcttttc 2940tttaagaatg ttgaatattg gcccccactc tcttctggct tgtagagttt ctgccgagag 3000atctgctgtt agtctgatgg gcttcccttt gtgggtaacc caacctttct ctctggctgc 3060ccttaacatt ttttccttca tttcaacttt ggtgaatctg agaattatgt gtcttggagt 3120tgctcttctt caggagtatc tttgtggcat tctctgcatt tcctgaattt gaatgttggc 3180ctgccttgct aggttggggg agttctcctg gataatatcc tgcagagtgt tttccaactt 3240ggttccgttc tccccatcac tttcaggcac accaatcaga tgtagatttg gtgttttcac 3300ataatcccat atttcttgga ggctttgttc atttcttttt cctctttttt ctctgcactt 3360ctcttctcgc ttcatttcat tcatttgatc ttcagtcatt gatactcttt cttccagttg 3420atcaagtcgg ttactgaagc ttgtgcattt gtcacgtaat tctcgtgtca tggttttcat 3480ctctatcagt tcgtttgtgg ccttctctgc attgattatt ctagttttcc attcttctat 3540tcttttttca agattttttg tttctttgcg ctgggtacgt agttcctcct ttagctctga 3600gaagtttgat caaccaaagc cttcttctct caactcgtca ttctccgtcc agctttgatc 3660cattgctggt gatgagctgc gttcctttgg agggggagat gtgctctgat tttttgaatt 3720tctagctttt ctgccctgct tttcccccat ctttgtggtt ttatctgcct ttggtctttg 3780ataatggtga cgtactgatg gggttctggt gtgggtgtcc tttctgtttg ttagttttcc 3840ttctaacagt caggaccctc agctgtaggt ctgttggagt ttgcttgagg tccactccag 3900accctgtttg cctggttatc agcagcagag gctgcagaag atagaatatt gctgaacagc 3960aagtgttgct gtctgactct tgctctggaa acttcgtctc agaggtgtgc ccagccgtgt 4020gaggtgtgag gtgtaggtct gcacctagtg ggggatgtct cccagttagg ttactcaggg 4080gtcagggacc cacttgagca tgcagtcagt ctgttctcag atctgaacct ctgtgctggg 4140agacccacta ctctcttcaa agctgtcaga cagggtcatt tacatctgca gaggtttctg 4200ctgctttttg tttagctatg ctctgtcccc agaggtagaa tctatagagg caggcaggcc 4260tccttgagct gtggtgggct ccacccagtt cgagcttccc agcagctttg tttacctact 4320taagtctcag caatggtttg cgcccctccc ccagcctcgc tgccaccttg cagttagatc 4380tcagactgct gtgcttgcaa tgagggaggc tctgtgggtg tgggaccctc cgggctaggt 4440gtgggatata atctcctggt gtgccgtttg ctaagaccct tggtaaagct cagtattagg 4500gtgggagtta cctgattttc caggtgttgt gtgtctcagt ttcccttggc taggaaaagg 4560gattcccttc ccccttgcgc ttcccaggtg agctgttgcc tcaccctgct tcagctcttg 4620ctggttgacc agcaccaatt gtccggcact ccccagtgag atgaacccag tacctcagtt 4680gaacatgcag aaatcacccg tcttctgtgt cgctcgcact gggagctgga ggctggagct 4740gttcctgttc ggccatcttg ctcaggatcc aggttttttc atgtagattc ttaactgata 4800aatatgttta acattattaa aatagattaa aataggttat agttaataca atgaaaattg 4860gcagtcctat tgcaaaacgt atgccataac tacagcattt atcactgaca gtcataccag 4920gatagtcttt tatttccaat cacttaaaca ttcctaattg caaaaaaaat ttgaagacta 4980aaattcagaa gttttgaaag agccattgcc tggataaatt atacaggttt cagttttatt 5040tataataatt atgtatatat ttataaatta atacataata aattaataac ctgtgatagg 5100caatgcaaag atgacagtga gagggcaaaa ttgataagat tgataaagtt atgttaacat 5160gaagaaatta accaggatac catccacact ataaagttag agaaatttat caggacagtt 5220ctcaaatact cttctcaatt ttaacaatgt aacaggaatt tttaagattt tggtattatg 5280tgtgtttcct tccagataat ttgaacagat tcatatttgg tattttttaa agccatatct 5340ttgtccttag tgctggcaat gtattcttga gaatgaacaa atcagaaata tgtaaaagcc 5400ataagtgagg gtgtcaggtt gaagtagtca atcaattata cagaacacaa agaattttat 5460cttgtacgat gttcacataa ctttacagaa gtgtgctgaa agggctataa aacatggaca 5520tgattatctc attgaaaggt ccaagaggta ctgaaataca tgcttttatt ttgaaccaac 5580caccctataa acgttgtatg gcttatttag atgagcgccc gtgtgtgtgt gtgtgtgtgt 5640gtgtgtgtgt gtgtgtgtgt gaatttgaca gggaagcaag agcatcaagt ttccaatgca 5700ctctgactgt ggttagaatc atgctgaaaa catggctccc ccagttctgg aatgagccca 5760cagatcaagc attcccagaa gacatagcag gctcaaatcc ctgtgtacac aatatttcat 5820gattatctta tgtcagtagt ttcaaagtat acagtttgtg tgaagacaaa tccaatgtca 5880tttttttttt ttttttgcta gcctatatgt gtggtaaatc cattatttat ttacttcctg 5940aaaattacaa tcagattaag aaactgcagc aaagttggca tgatgagata gagactgaag 6000tgtaagctta tgttaatgat gcccttggtt tggataaaca catctaagag aaaactgaaa 6060aaacacacat ggcagggaaa ccttgataga gtctaaatat aggattgtat gtagtaatgc 6120aatcaacaga tgagcatttg gcagtaatat tatttttcaa atatggataa acactgctta 6180ggagagtaaa gagagacaaa gttgaaagca ggtttatagt aggtgttgtt ttagtgttga 6240tccctttttg ctccaataat caaagtgata aatattgaaa atcgattcat gcagcattac 6300ttgttccatt ctaattttta tatatgtcaa cagtgccatc tcccaacctg tgctgtcccc 6360ttcaggagaa gtgactgtgc tgaagtttat aaggttgaca tattgccagc ttcaatagta 6420aagctgaagt gtatactgaa ttcttaatgc aaataacaac tctattggaa agtaacccag 6480ttatagaagt gctaatttgt caggagctgc cttaccaaga tcaatgatga cgatagttat 6540ctcaggattc tgaaagattg ttttcagatt tcaactagtc tagctgaatg ttccttaata 6600gaaagagagg acttttagaa ttggttcaat atgatgacct cctgaattat ctcacatagc 6660ccctgtgtac atgcctttct tttctctcag aaaatggcac tatcataata gctttcttac 6720gcagacttca ccttagggtt ttacagtaag ggaggggtct ggtgtttcat ttattttgaa 6780ctatttgttg ttgattgtgt acagtgcttg agtaaaaaat ggaatatagg aacatctaga 6840ataaattttt taaatgatca ggtgactaca aagtgaatta ttagtgtcaa aatgttggga 6900aagttttaag agaatatagg aaacttggac attacacaat tacactggga ccaaattgtg 6960gggtctcatt atagttagta tttatgtatt tttttccaat tgatttgtgt gccttttttc 7020tgcatgtttt tggcagatag aatggctata acaagtaaga gtatgtcagg aaataaaaat 7080aagcagaacc ctattccttt aaaattcttc atttgatggg agggtcataa aataagtctt 7140aacacattta aagaattaaa ttcatgtaaa ccatgttaat ttaattccac aatggtatcg 7200aattagagta agaggaatat ctcttactct cctaaatctc ctaatgtttg gaaatttaaa 7260ttagcatttc tggcctattt attggtttaa aaaaagacaa aaaaaaagga aaattgaagt 7320attttgaact gaataaaaat aaaaatatag aatctaaaac tgtatgggat actgataaaa 7380caggatatag ggagaaattt atagcactga aatgcctata ttagaaaaga aaacaggttt 7440taaatcagta aatttgtagt ttaccttaag aaacttagaa aagaacaaat taacccagag 7500ttaagtaaaa taaaagcatt aataaaggta agagcagaaa tcaatgaaat ataaaacaac 7560aaaacacaga gaaaatttga gaaaattaaa aaatagccca gtgagaagat attgataaaa 7620cttgtaacca gaccaattta agaaaaaaag tcaaaacaca aataccaata tttgaaaatg 7680taagagggta aatcattaca gattctatga atactaaaat gacaataagg aaaaattatt 7740taaaaggggc atgtaagcca ggcatggtgg cttacccctg taatcccagc actttggcag 7800gctgaggtgg gaggattgct agagctcagg catttgagac cagtctgggc aacatgttga 7860aaccttgtct acacaaaaag tacaaaaatt agctgggtgt ggtgttgcat gcctgtaatc 7920ccagtcactt gggaggctga gatgagagga tcacttgagc ccagaatgtt gaggctgcag 7980tgagccatat ttgcaccact gccctccagc ctgggtgaca aagcaagacc ctacgtcaag 8040aaaaaaaaat gtatgccaac atttttcaat aacttaaatg aaatggagaa attccttgaa 8100agacacaaac tacaaaaact cagtgaagaa gtaaataacc tgaatagccc tgtatcaagt 8160aaattgaatt tgtaattaaa agtcttccaa cagagaaaac ttcaggcaca tctagcttca 8220tatgaaatga aaaaaaaaaa aaatgctaat cctctacaac attccagaac atttagaaga 8280agggaatatt tcccaactta ttctatttgg acagcaatac ccaggtaaga aaaaaaggca 8340cagaaattta aaaagaagaa tatacattat tcctcatgaa cataaatgca aagaaatcta 8400atcaaaattt tagcagatga aatgtagaaa tactttatga ccaagtgagg gttacccaag 8460gaatttaagg ttggttttat atgtaaagat caaccagtgt aggaagatca cttctggaaa 8520gtcagagtaa gagcctccaa aaatctactc ctccataaaa ccaataagag ccttgacgga 8580aatagtcgaa attaattttc taaaactttg gaaattaacc aaaggcttac aaaattccaa 8640agaacattta tgcaagaaaa atggctgaat cagtaagaac agccagcttt gtggcatttt 8700aatatgcctc cttcccatgc ttttctccct agtactgtag tagtcttaaa aattagcagc 8760atagcaacca ctggagaaga agaaaggttt ggaaatttcc taaaaagttc cgtccccata 8820taattatcac tatttgacct ctaaagccca atctatatga tttatattca tttggactga 8880ctcagtgctc actaagtaag gaaatctcaa tttcaaggta ttggtcaaaa agaatccatg 8940gcaattgttg actatcacaa ctgcctgcag tgttggtaac tgaattggga taaacaagaa 9000gctgatcaaa aactgaaaac taaaatcttg ggaatgagat gtctagaggc tgtttcaaaa 9060agctctgaca cattcctttt tatctagaaa gccacatgca ggctgggcgt ggtggctcac 9120acctgtagtc ccagcacttt gggaggctga ggcatgcgga tcatgaggtt aggagtttga 9180ggccagcctg accaatgtgg tgaaacccca tctctactaa aaatacaaaa agtagccagg 9240tgtggtggcg cgcatctgta

accccagcta ctcaggaggc tgaggcagga gaatcgcttg 9300aacccgggag gcggaggtta cagtgagcca agatcacgcc actgccctcc agcctgggcg 9360acagagcaag gctctgtctc aaaaaaaaaa aaaaaaagaa aagaaaaaag aataaaaaaa 9420gaaagccaca tgcatgtaat tgtttacctc tggctttcct tttatgctct gggcaagtta 9480aggaagagtt gtgaactacc taagtgctga atgggaacca taacacacac acacacacac 9540acacacacac acacacacac acagcacctt aggaaagggt gagaagcatg ttagttagaa 9600gcattaaagg aaatctcttt ctagtcatta tctgtgcact ataactgagc agagacttca 9660gtatccacat actacagggc atataaactt tacagaatta gtccgggaaa atcatgtgaa 9720aaaaaaaaca gcaacaaaaa aaactctggg aaaggggaga atatgatttg aagagtcgcc 9780acattataca taatatgtct attgttcaac aaaaaagtac gagacatgca aagaaacaga 9840aaaatatggc acaaatagga taagatgaag tcaatgaaat tatccttaag gaagaccaga 9900tgttgatctt agtagacaga cattgaacca gctattaaaa atacgtacat agaactaaag 9960aaaaacatgt cgaaagggtt aaagaaaggt ataaaaatac tgtcttacca aatatagact 10020accaataaag agagagaaat tatgagaaaa gacaacatga aaaaatgtaa agcaaaaaaa 10080ttagacagtt gaaacaagag ggcctctatt tgcagatttg agcaggcaga agaaagaatc 10140agtgaacttg aagatacgtc aattgagatt atccagtctg agcaacagag gtgggaaaaa 10200atgaagaaaa ctgagtaaca aagaaccgta gaacagcgtc tctcatgcca gtggatgcat 10260aaactggagc cctagaagga tgaaaaaagg agaaggaaag ataacttccc aaattttatg 10320aaaaacatga atttatatac ccgagatgac caataaaatc caagtaagat actctcaaag 10380agatcatcac ctagccacat cacagtcaac atgtcaaaag acaaacaagg agagaattct 10440tgaagatagt aagaaaaaaa ttattcataa catacacacc atcctcaata agtctgacaa 10500ttgacttctt accataaacc atgcaggctg aaaaggcaat gtacataacc caagtgatga 10560aataaaaact ttcaaccaat aattctagat tcaacaaaac tattgttcaa aaagaagaaa 10620tgaagacatt cctaaacaaa atctcagaga aatgttctct ataagacttg tcctaataga 10680aatgctaaag gaactccttc actctgaaat agaaaggcac tggagagtaa atcaaatcca 10740cgagaagaaa taaagagaac cagtaaaact acatgtgtaa gtttaaaaca aagtataaat 10800ttattttgtt tataacattt gtctttttcc tatttgactg aaaacataat ctcaattata 10860aatttgtgtt gatggcatta tataaagatg taattttggg taatagcacc aaaatgtcag 10920aataggagtt ttctgtaatt gttttcccac ataagtatca attttgacaa ccattcatga 10980gcaagagtac cttgtgggag ttcaggagtt gacagtaaaa gttcagcaca ccagtggagt 11040aaaaaaatct aagaatagat tcattggaaa gggtataaac agtttcactt tacctgcatc 11100accctcccca aaaagtggca cagctcagta acaagagcct gttatttctt ccacagggga 11160aaacaagagt ataataaatg agtatccagt ttctcaagac atacaggccc ctgcccaaga 11220gatccacttt attttcatct cacccagaat actgaggtga tcagcaaggt ggagtggttg 11280ggagagggta aagcaggaaa gagagatgag gactcacagc aacccacact tgaaactgcc 11340atagatccta ccagttgctt catggactcc atcaggagcc cacctgtaag ccacttggga 11400tgcaaaactc ccatcttgtc aacaaaaccc cagatgctcc aaatgcctcc cccactcttt 11460ggctccccaa gcacactcct gtgaaaagca gcgagaatct gcagatggct tgcaagcact 11520gttgacagct ggctgcactc tgcagaactg gaagaaagct cacaaacacg acaatttcag 11580gacaccaccc taaaaaaatg agacttcagc acctggcctg cctttatgca acctggagaa 11640ggtgatatgg tttggctgtg tgtccccacc caaatctcat gtcaaattgt aattcccact 11700tgtcagagga ggggcctggt gggaggtgat tgaatcatgg gggtggactt ccctcttgct 11760gttcttgtga taaagttctc atgagatcca gttgtttgaa agtgtgtagc atatccccct 11820tcattctctt tcactccacc ttggtaagac atgcttgcta ctcctttgcc ttctgccatg 11880attttaagtt tcctgaggcc tcccaaccat gcttcctgtg cagcctgcag aactgaactg 11940tgagccaatt aaacttcttt tcttcataaa ttatccagtc ttaggtagtt ctttatagca 12000gtgtgagaat ggactaatgc agaaggcata caaccttaag aatctgcccc cttgagggaa 12060caagatgtgt ggagcaggtt catccataga caatgtctga gagaacctca aaatccctag 12120cctgactaac tgatgaaaat gtttctctcc taaggccagt cagtaaagac cagagggggt 12180gactctttct ttaaatgcaa aggcagcagt acagtaattc aagaaacatg aaaaatttta 12240aaaaatgaca ccaccagaag aacacaatca ttttccaata accaactccc caaaaatgga 12300gatgttcaaa ttggtttata acaaattcaa aacaattata ttaaggaagc tcagcaaact 12360aaaaggaaca ccaatagact actctgtgaa atcaggaaaa caattcatga acaaaactag 12420aaattcaaaa aagagaaaaa ttatcttaaa agaaaaccca gaaattatgg agctaaagaa 12480tacaatgcat gaaatgaagg agcgtataca acagcaacgt tgatcaagca gaagaaaaaa 12540atctgtgaac ctgaagactg gctatttgaa agtattcatc agaggattaa aaaaaaaaga 12600atgaaaagaa ataaagccta caggatgtat aaaacatcat caagagaact aatatagaga 12660ttattggagt cataaaggag aagagagaaa agggcagaaa acttatttaa gaaataatgg 12720ctgaaaactc tccaaatcta ggaaaagata tgagcattca ggtatatgaa gctcaaatat 12780ccccatacag gatacattcc aaaaagactt caccaaaaca cattataatc aaactgtcaa 12840aagcaaaatc aagacaacga ataaaccaat gcaaccacca atcactaaga gggagacagg 12900attcttatgt tgtgattatt atttacatat aatttcagta agtgttatta gaaaatttat 12960aatgttttta aaaaagaaat ttgaaagcac tgaaagaaaa gagactcatc acatacaggg 13020aaccctttta agtctatcaa cagatttctc agtagaaacc ttacaaaata ggagacagtg 13080ggatgaacta ttcaattgct acaaggaaaa aaatgccaac caatgcttta cctagcaaat 13140ctgttcctca gaaatgaagg agagataaga actttcctag acaaacaaga ggcagttgtt 13200agatgaaaaa gaggcagttc atcaccacta gacctgcctt acaagacata ctaaggagag 13260ttcttcaagc tgaaatgata tggcactagt tagtaatatg aaatgataaa cctcactggt 13320aaaggaaagt acaaagtcaa atttagaaca ctttgatact ataatgatgg tgtataaata 13380attttactat actatgaagg ttaaaagaca aaagtattaa aaaaaaaaaa acatagctac 13440aatagcttgt caatgcatac tacagtataa aaagatgtaa attagaacat taaaaacata 13500gcatgcaagg gtagggaagt aaaagtgtag ttttcatatg taatcaaatt taatttgtta 13560tcagcttaaa ataaattgtt atacctataa gatgttttat gtaagtgtca tggtaactat 13620aaagcaaaaa cctctagtag atatacaaaa gaaaaagaga aaggaatcaa aacactacag 13680aaaatcatca aattacaaag gaagacagta agggaggaac aaagtaagta aaaagagcaa 13740gaaaaaattt aacatgatga aaatagtaag tccttacctg tccataatca ctttaaatgt 13800aaacggattg aattttccaa acaaaaacag aatggacaaa tggattttag aaacaacaac 13860aacaataaac accacacaca cacacacaca cacacacaca cacacacaca cacacacaca 13920cacacacaca cacacaccca gacccaacta tgtgctgcct acaagagatt tacttcacat 13980taaggacaca tacaggctga aatgaaaaaa atagaaaaag atactgcatg cagatagaaa 14040ccaaaagaga gcagaggcat ctatacatat agcatacaga aaagatttta agttaaaaac 14100tatatcaaaa ggctaaggtc aaaatcgtga agcagttaat tgttccagaa gacataacaa 14160ttgtaaatgt ttattcaccc aacgttgaag cacctaaata tataaggcaa atattaatac 14220atataaaagg agaaatatac agcaatacat taatagtagt gaacttcagt atctcccttt 14280caaaaatgaa tagataatcc agacataaaa tcaataagga aacatttaac ttaaacttca 14340ctttagacca aatggatcta acagacatat agtgaacgtt tcatccaaca gtggcagaat 14400tcacattctt ctcaagcaca catagaacat tctccaggat agattatatg ttagatcaca 14460aaataatatt acaaaattta ataaagctga aatactatca attatttttc accacaatag 14520cataacacta gaaatcaata ataagaagaa aactggaaat ttacaaatat atagaattaa 14580catattcctg aacgaccaat gggtcaaaga aaaaaatcaa aataattttg tgacagcaaa 14640gtagaaacac aacataccaa aacatacagg acacagcaaa agcagttcta tgaggtaagc 14700ttatattaat aaacacattt ttaaaaagat tttaaataaa caacattaca cctcaaggaa 14760ctacaaggaa ggaaaaaaaa aacaagcccc atgttatcaa agggaaggaa ataacaaaac 14820tcagacagaa agaaatgaaa catagactag aaaaacaata gagaatatta acaaaactta 14880tagtttttta aaaaaattaa atcaacaaac ttttagctag actaaaaaag agaagactca 14940aaaaatgaaa gaggagacat tacaactgat accacaggca atacaaatta agagaaaact 15000atatgccaac aaattagtta acttgcagtg ggtaaatccc tagaaacata caacctacaa 15060aaactgaatc atgaagaagt ggaatatctg aacagatcaa taatgagtaa gggaattgaa 15120tcaatattca aaaatctcac aaaaagaaaa gctcaggatc agatgacttc actggtgaag 15180actaccaacc atttaaaaaa gaattaatac cactctttct taaactcttc caaaaacatt 15240gaagaggaga aaacactttc aaattcatta taagaggcca gttttacctt gatatcaagg 15300atttaaagag aacactttga gaaatgaaaa ttacaggcca aaaaccctga taaatataga 15360tgcaaaaatg ttcagcaaaa tactagcaaa cctaattcag caacacatta taatggcata 15420catcatgatc aagtgagatt catgcctggg atgcaggatg gttcaatata atcaaatcaa 15480caaatgttac actgttttaa caggatgaag gataaaaatc atatgatcat ctcaatagat 15540ttagaaaagc atttgacaat attcaacatc ctttcaggac taaaaaccct caataaatca 15600ggtatagaaa gaatgtgcct caacacagta aaagccacat atgacaaacc tggaggtaat 15660atactcagtg gtgaaaagta aaaagctttg accctaagat cagaacaaaa acaaggatgc 15720ccaatctcac cacttatatg taacatagta gttgaaattc tagccacacc aattagggaa 15780gaaaaaaggc acccaagttg gaaagaacga agttaaattg tctctgtaga tgacaagatc 15840ttatatgaga aaacactaaa gactccacca aaacgctgtt ttagttagag cttaaaaaaa 15900gaattcacta aagttgcagg atacaaaatc aatgtacaat atcaagttgc atttctaaac 15960accaaaaaca agttatccaa aaaattaaga aaacaatcct atttgtgata tcatagaaaa 16020tactaaagaa taccaaagaa actgaaaata aatggaaata aatggaaaga taacctatgt 16080tcatcagatg aattaatact gttaaaatgt tcatgctacc caaagcaact tacagattaa 16140gtgcaattcc tactaaaatt ccaatgacat ttttcacaga aatagaaaac acagtcctaa 16200agtttgcatg gaactgcaaa agactcaatg aagcaatttt gagcaagaat agcaaagctg 16260gagacatcac accacctaac ttcaaagttt attaatcaaa acagcatggc ataaaaacag 16320acagaaagac caatggaaca gaatagagag cccagaaata aactcacatt tatagagtca 16380actaacattc aacaaagcag ccaagagtac acaatgggga aagtatagtc tctacaataa 16440atggcactgg gaagactgaa tatccaaatg caaaagaatg aaattacacc cttatcttac 16500accatacaca caaatcaaat caaaattgat aaagacttaa atataatacc tgaaaccata 16560aaatctttag gggaaaactc attgacattg gtcttggcaa tgattttttt gatatgacac 16620cagaagcaca ggcaataagt gggaccctga caaactaaaa tgtttctgca cagcaaagga 16680aacaatcaat catcagaact aaaaggcagt ttatgaaatg ggagaaaatg tttgcaaacc 16740acatatctga taaagggtta atatccaaaa atatataagg aatgtataaa attcaacaga 16800aacaaacaaa taatccaatt ttaaaatagg tgaagaacct gaatagacat ttttttcaaa 16860gaagacttac aaataggcaa caggcatatg aaaaaatgct tgacatcact aatcatcagg 16920gaaatgcaaa tgaaagctcc agtgaaatac cacctgtaac tattaggatg gctattatca 16980aaaactcaaa agaaaacaag ctgggggaat gtagagaaaa gggaaaagaa ggcccttata 17040cactgttggt gtgaatttaa actggaatag ccattatgga aaacagcatg gaggttcctc 17100aaaaaattaa aaatagaact actatatgat ccagcaattt cactattgag catatatcca 17160aatgaattca aatgactgtc ttgaagaggt atttgcacac tcatatttat ttcagcatta 17220ttcccaatag ccccgatatg gaatcaacct aagtgttcat cagtagataa ttagataaag 17280acaacctggt atatagacac agtggaatct atttagcatt cacaaagaag gaaatccaac 17340ctttaaaatc ctttaaaagt taaactcata aaaacagaga ggagaatggt ggtttccaga 17400aactggaggg tcagggaatg gggagatgtt ggtcaaaagg tacaaacttt cagttataaa 17460atgaataagt tctagagatc tagtgtacaa cagcattact ataattaata ataatatttt 17520gtatacttga aatttgttaa aagagtaaat atcaatattc tcaaatacac acacaaaaat 17580agaactatct gaaggaactg atatgttaat tatcttgatt atagaaatca tttcatgatg 17640tataatgaat atcaaaacat aatagtgtac atcttaagta tatacaattt tgatttgtta 17700atcatacttc aatgaagcta gaaaaaaata tgtaattttt aagacagtaa taatataaat 17760taagggtgag ggaactgact tatgttggag caaagttttt gtaaactaat cagtatccat 17820tcaagctaga ttattttaaa ttgttaattt aattgtaata ctaaggcaac cactaaaaag 17880ggctttaaaa aatacagtac ttgaggctgg gcacagtggc ttatgcctgt aatctcagca 17940ctttaggaga cctaggtggg tggatcacaa ggtcaggagt ttgagaccag cctgatcaac 18000acggtgaaac cctgtctcta ctaagaatac aaaaattagc cgggagtggt ggcgggcacc 18060tgtaatccca gctactcagg aggctgacgc aggagaatca cttgaactcg ggaggtggag 18120gttgtagtga gccgagatca caccactgca ctccagcctg ggtgacagag cgagactcca 18180tctcaaaaaa aaaaaaaaaa aaaaagctaa tttcattagc tatatgaaat tctgagagac 18240gaaactgaag tctccaacta taattgtgga tttgtctatt tctcctatca gttctatcag 18300tttttgcatc acatatttga ggctctgttg tttggtgcat acacaactgg aataatcgtg 18360ccttcctggt ggcttgatta ttttatgatt atatagagcc tatctttgtg gtatttttat 18420ttactcttaa atctactttg tgttatatta atatacccat tcttttttta aaaaaattgt 18480ttgcaagata catcttttcc attcttttaa tctcaaccta cctgtgccat tgaattcgaa 18540gtgagttttc acatagagaa catactattg aatcatcgtt ttaaaaattt catttgccaa 18600tcttctatat attgaggtaa aattcacata aaatttatca ttttaaggtg tacaatacag 18660tgacatttag taatacacgt tatgcaatat taactctacc tgattactaa gtgttttcat 18720catccaaaga aggaaacttt atactcatta agcagttaat tcccattcct tctccctgcc 18780acgggcaacc acaaacctac ttttctatct ctatgaattt acgtattacg gatattttgt 18840ataaattgaa ttatacaata agtgaccttt atgtttggct ttgttcactt agcatactgt 18900gttttgatat tcaactatgt tgtagtatgt ctccgtttta tctgaataac tcagttcttt 18960ttgttgtata cctaaaagtt gattcgtagg ttgtaataat aattctatgt ttagtttatt 19020gaatagctgc caaagttttt ccatagtggc tctgtcattt taaaatccca ccagcaatgg 19080atgagagttc cagtatctcc acatccttac caatatttat tattttatat ttttacgatt 19140atagtctttc tagtggatat gcaatggtat ctcattgtgt ttttggtttg cctttcccta 19200atgactaaca atgttgagca tcttataata tacttgttag ctatttgtgt ttagagaaat 19260ttctattcaa gtgccttgcc cattttaaaa actgagttat cttgtcgact tatgagttct 19320ttaatacagt aaactcttat cagatatgac ttataagtat tttatcccat tctgtaggtt 19380accttttcac ttttgtgata gtccattatg cacaaaggtt ttgatttttg ataaatccaa 19440tttatctgct tttgttgttg tttttgttgg tggtgctttt gcaaaaccca gtgtcatgag 19500gtttttctcg ttatctttgg agaattttat agtttgggtt tatacatgta gattattgat 19560ctaatttcca ttcatttgtg tgtatgctct gaggtagggg tccaaattca atttttgcat 19620tgaattgaaa attcatattt caatttcata tttcaactgc atattcagtt gctgcagcac 19680gatttgttga agagatcatt cctttccacg gggaatgatc tgcgactctt atcagaaatc 19740aattgatcat agatgtatgg gattatttcg gactttagat cttgttccat gaatatgcct 19800atttttatgc cagcaccgca atattttcat tactatagct ttatcataca ttttgaaatc 19860aggaagtatg tatcctccaa gtgtgacttt catttgctag actgttttga ctatttgggg 19920tctttgcaat tccatatgga tttcagaatt gacttttcat ttaaaaaaaa tggtagttga 19980gattttcata ggaattatat tgaatctaca gatccctttg ggtagtattg ccatcttaac 20040aatattgtat tccagcccat gaacatggat gtattgccat ttgtatcttt gttcttttct 20100ttcagcaaca tttagtatat gacacttgta actccttggt taaatttatt cctaggcatt 20160ttatcctttc tgatgctgtg taaatggcat tattttattg atttctcttt tacattgctt 20220gttgtaggtg tatagaaata caactgatta ttgtgctttt attggattat ctgcaacttt 20280gctgataatt tattattagc aacatttatt tattagctct agtaggtttt ttctttttct 20340ttagagttgt ctatatatgt tgctctgtgg ataattttac ttcttgatct ccgatatgga 20400tacctttatt tctttttctt acctaattgc tgtagctaga attttcagta taaagtatga 20460cagaagttgt tcacagctct ccttgtcttc tctctgatct taggggtata gctttcagtc 20520tttcaccatt aagcacagtg ttagctgtgg ggttttctta gatgcttttt aatatattaa 20580ggatgtactc tgctatttct aattggttgt ttttattata aaatggtgtt agattttgtg 20640actttatgtt cttcataatt gagattatca tgtggtcttc ttttcattcg attaatgtgg 20700tatattatat gattttcata tgctgattta cctttacatt tttggggtaa accccattgt 20760gtacacaggt tttttgaggg tctgctaatt ttcttcactc ttttctctcc ctgagacgga 20820gtaatgttaa ctgacttatc tttaagttta ctattttttc ttctgctgct caaatctgct 20880tgaacctata tagtgaagtt tttattttct cattgaactt ttcagcttca aaatttcttt 20940ttggttactt tttataatat ctgtttcttt acagatattc tctatttgga aagacatttt 21000tctcctggtt ttctttactt atttgtctgt atttttttaa agtctttgag catatttgag 21060gcagttgatt taagtatttg tgtaccaagt ccattgcctg agcttttgca tagagatttt 21120ctattaattt cttttttttt tttctgtgaa caggcctcat ttttttttct agaaaattgg 21180acattttgag tattataaca acatggctat tttagaaata actctcctcc ctccttaggt 21240tttgtttctg cttgttgcat gttgctgttg tttgctcatt agtgattttt tctaaagtat 21300tttgaaaagt ctgttcttga tcatgtgggt ccattgaatt ctgtgttctg ttaatttcat 21360ggtcagctag tgttttgaaa gtttcctcaa gtgcgtacag ccaataagag aaaaagaaaa 21420gaaaaacaga aaaagagagg aaaaaaggaa gagggaaaga gattttaaaa aataatgttg 21480gccgggcgcg gtggttcaga cctgtaatcc cagcactttg ggaggccaag gcaggctgat 21540cacctaaggt cgggagttca ggaccagcct ggccaacatg gagaaaccct ctctctacta 21600aaaatacaaa attagctggt gtggtggcat gtgcctgtaa tcctagctac tcgagaggct 21660gaggtaggag aatcgcttga acccaggagg cagaggttgc agtgagccga gatcgcgcca 21720ttgcactcga gcctaggcaa caagagcaaa actgtctcaa atacatacat aaataaataa 21780taataataat aatgtctgct gattggctct gtttgggccc tcattcaatg cctagtcagg 21840ccatttacaa ctctcttaac ttctactact tgcttgtgta ctgactaaag gccagctata 21900ggtgaaagct taacctcatc tcagatctta taggtgaaag cttaacatca tctcagatct 21960ttttggacca tgaatcctac cctgggtatg cacatgatct tcttaatttc ccagtagatg 22020caagagtttt aataaatttt aaaagtcctt attccctcat gtatctcctt ttccgacctt 22080ttcagtctgt ttattgttca tctgaactga catcctttgc cccaggctgc tgtggcaaaa 22140acttttacct ttaaatgctt tcaccacaag ccacctggga agccgcccca gacctgggac 22200tgctctgacc ctgaggaaac aaagacaaga ccttgcacag ccaggcagcc ataagacaag 22260tccacaccca aaccccagtt ctttcagaag aaggtctgcg ttggattctc tggccctagt 22320aaccagcaaa atactttgtc aagtattttt atcagtctgt ggttatcttg tctttctcag 22380agcagttttt aattttaata aaatccaatt tgtcaattac ttatctcatg tgactctggt 22440gttacatcta aaatgttatc accatacttg aggtcaccta ggttttctct aggaatcgta 22500tagttttgcg ttttatattt ggtgtatgac acattttaag ttaacttttg tgaggttgtg 22560aggtctgtac ctagattcat tttgttttgc atgttacttc aatattcata agaaacattg 22620gtctataatc ttctttctta tagtatctta gccttgcttt agtttttggg caatgctggc 22680ctcactgaat aaattcaaag tattccctct tcttcaatta tttggaaaag tttgagaaag 22740actagtgtta actgttttct aaatttttgg tagaatttac cagtgaacca actggtccta 22800ggcttttctc tagtaggtgg ttttgattat gctttcagtc gctttacaag ttatatatct 22860atacagattt ttataattca gtcttggtag gttgtgggta tttaggaatc tgaccacttc 22920atctaagtta tccaattagt tggcatacaa ttgtttatag ttctataaat aatcattttt 22980atttctgcaa aattggtaat atcccagttt ccattttttg tttcattgaa tcttttttct 23040tagctcatct agctaaatgt ttgccaattt tgttgatctt ttggaagaac caacctttga 23100tttattaatt ttctctattc ttttttctgt tctttatatt atttatttcc acacgaatct 23160ttactatttt cttcattctg ctgcccttta atgttttttt ttaatttttt atttttaagg 23220tggaaattta ggttgagaaa tttttttaaa tgaaagcatt agagctatac attttccttc 23280tggtgttcct ttcacttacc tgccataaat tttgataagt tgagtttttg tttgtcttgg 23340ggtattttct aatttctctt ctaatttctt tcttgaccca ttggctattt aaatgtattt 23400aattttttac atattgtgga tttcccagtt ttccttctgt tattgatatc tagttttatt 23460ccattgtgat cacagaagat attttgtata atcgcagtct attaacattt attaagcctg 23520tgtcgtggcc taacagagga tctatgttgg agaatgttcc aagtgcaatt gagaatacta 23580ttctggtgct attaggtgaa atattctcta tacgtctgtt aagtccaatt catctatagt 23640gttgaagttt tctgctcctc attgattttc tatcttatta ttctatccat tattaaaagt 23700ggagtagtaa agtctcttaa ctattattgt agaactctct gtttttcaag tcccagctct 23760gtttgctgca taagtgttta caattgttgt atctttttgg caaattgacc agtttcataa 23820acgtaaaatg tacttcttac tgtcttctaa cagttttttt tttcttttta cataaagcct 23880atcttttctg atcttagatt cccttacact ccaccccagc tcacttttgg ttacatttac 23940ataatatatc ttttccatcc tttcattttc aacctgtttg tgtctttata tctaaagtga 24000gtgtcttata gacagcataa actatgtcat ttaaaaaaat ccattttgct tatttctgcc 24060ttttgactgg ggagtttaat ccatttgcat ttaaagtaat cactgatcat gaaatacttt 24120cagtattttg ttaaagtttt atgtatgtct tataactctt ttgctcttca tttccttcaa 24180tattgccttt gtgtctagct tatccttttg tgtcacactt tgatcccctt ctcatttctt 24240tttgtatatt ttctttgtgg ttaccacgag gacaatgtat caacttttaa agttattaca 24300attttattgt ttaaaatctc

tcccattcag ggattttaga aaggttaaat aataatgtaa 24360atgagatatc tagaacaata taagtattca ggaattagct caattccaat cctttctcca 24420cctccacctc tttctctgtg agattataga aaagatgaca aaaaggatgt tttctgagcc 24480ctttaattgt tgagaatgat ctttgagaaa aagaaaaaaa atgaaagcat taggattgta 24540caacagcctg gaagtataat taagtgtaaa ttaaatagat aaaagttata agcagaggaa 24600agtatagtag aactcagtat ttaaaagaat caatgtgaaa actatataca tttatataaa 24660ataaaaccac cagacaaatc tgatatcctt aggatttttc tttctttcat gtgatttcta 24720attgatacat atgacactaa accattgacc tgagctgtaa gagaaactgg aaattgttct 24780gttatctttt gtaggatttc tagaactttt tgctctcaga cttaaatgcc aatggattcc 24840tacttattgt ttactgcttt tggacttaca tatgatttca ttctttctca tctcttatcc 24900ttacaatgta atttgaactg atgccaattt aagttcaatt gcatacaaaa actctattcc 24960tatgcagctc tgccccatct aatttatgtt attgatgtca caaattacat ctttgtacat 25020agtttacata ttaacatgga tttataaatt tttatgtatt tgtttttaaa atcctgtaga 25080aaacaaaaag tcaacacatc aaaattaaaa taatactggt ttttatattt gtctgtgtgc 25140ttacctttat caatgttctt tacatcttta tatgggtttg agttactgtc ttgtgtcctt 25200taattccaac ctaaagaact ccctttagca tttctggtag ggcaggtcta gcagtaatga 25260actctctgag tttttattta agaatgtctt aatttctagc tcctgttcaa agtaaagttt 25320tctgtatata caattctctg tccattgatt tttgttcatt ttcctttcag ctcttttaaa 25380tatattttcc cactttcttc tgccttccaa agtttctatt aacaaaattc agcttataat 25440cttattgaag atctcatgta catgagtggc ttctctcttg ctgttttcaa gattctatga 25500ctttggtttt tgatagttta aatataatgt gtcttattgt gggtcttttt ggatatatcc 25560tagagtttct tgatcttctt agattggtat atccatgtat tttaagacgt ttgagtagtt 25620ttcagccatt atttcttcaa acaatctctc ttctttgggg acttccattt tgcccatatt 25680ggttcttttg atggcgtggc accagtcccc tagactctgt tcactttttt ccagtctttt 25740atttctaatc ctcagactca atagcttcac gtgtcctgta ttcaagtttg ctgattctta 25800cttcttccag ctcaaatctg ctgttgaacc ctcccttgta acatttttaa ttccattttt 25860gtatttttca agttaagtat ttttattagg ttcctcttta taatttctct ttgttgatat 25920tctcatttta tcacacacaa tttgtctgat ttccattagt ttttttcttt gttttccttt 25980agctctttga acatatttaa gaccttttta aagtctttat ccaagttcta tttctgtgct 26040tctgtggaga tattttctgc cagtttatgt tcttcttttc catgggccat gttttcctgt 26100ttctttgtat actttctaat tttgggttga aaactgagca tttgaaaata gagccaactt 26160cccagtttcc acagcgagtc tttatgccac agtatgcttt cactgattaa ttaggtatat 26220ctaagcttag ggagcagctg agtcaaaagt ttaaggtctt ctcaggtctt ttctgaatat 26280acatgtttcc tatgcctgtg tgaaacgttc tcaatttccc tatataaaca gctttttttt 26340tttttttttt ttttttgaga cggggtcccg ctctgtcacc caggctggag tgcagtcatc 26400tcggctcact gcatcctcca cctcccgggt tcaagcgatt ctcctataca gatgtgtgcc 26460accacatctg gctaattttt gtatttttag tagagacggg gttttgccgt gttggccagg 26520ctggtcttga acgcctgacc tcaggaggat tacaggcttg agccacagcg cccagcctaa 26580acagctactt ttgaatgcct taatttcctg aatagtctca acccagtttc tccttgaggt 26640cttaggtggt ccattgtgtg tctccaccca tagtcgcttg ccccaggcat ctgtgggtct 26700gtggtaccac tgcagctttc accacccgta gctgccactt tttcccatct gagatcccag 26760ttaggtgaga gaggtcattc cttcacgcag ccccttgaca ggttagaaca tttcaaataa 26820ggtctgttct gctcctctgg ttgaagggag agaactggaa accagtttcc caccttctac 26880aaaccaagat ctcatggtgc cacgggagtg gcaggacaag tgcaagtgaa aatgccatac 26940aattttctac cattttgaac gcgggttttt cttcattggg catttgcttg gttgctgtag 27000gcttttcagt tttccagagc tcacacaaga ttactttagc tggttttttg ttctttcctg 27060atgcttccct ggcagaataa gggttggaat ttccaccatt ttgctgattc ataactctgt 27120agtcagtttt aaatatactg atacttgagt ttgttttatg gcccagaatg tggtcttggt 27180aaatatttca tgtatactca gaaagaatgt gtattctgat gttgttacct gggctattct 27240ataaatgtta cttaaggtgg ttaataatgt tgctcaagtt ttctatagtc ttgctgattt 27300tcggtacatt cattccacca atgactaaaa caggtgtatc aataaaatct gttccatcag 27360gttttgcttc aggtattttg agggtctgtt attaggtgca taaacaagat ggttatgtcc 27420tattcttaaa ttagcctctt tatcattatg aagttaactt ttttgtttgt tttttgagac 27480agagtctcgc tctgttgccc aggctggagt gcagtggcgc aatctcggct caatgtaacc 27540tccgcctcct ggtggttcaa gcaattcttt gcctcagcct cctaagtagc tggggttaca 27600ggtgcctgcc accatgcccg gctaattttt ttgtattttt agtagagatg gggtttcacc 27660atcttggcaa ggctggtctt gaactcctga actcttgatc cacccacctc ggactcccaa 27720agtggtggga ttacaggcgt gagccgccgt gcccagcctg aaataaactt cttagccctt 27780gctaatgttc tttgctctga agcatgcttt gctggtatta atatagtcat tcctgctttc 27840tttgattcat gttcgcaggg tatatctgtt tccattcttt tacttttaac ctattgtctt 27900tatatttaaa gtgcatttct tgtaagtata attggtttct caaaatccag tctgacattc 27960tctgcctttt acatgtcatt tttatatgat ttgtgtaaat atgattatta ttacagctaa 28020attgaagtct gtcatcttgc tatttggttt ctatttatcc catttttccc tttttttttt 28080tttttttttt ttttgctttt cttgagattg agcattgtat tagttttcta gggttgctgt 28140aggaaagtga cataaactgg atggcttaaa acaacagaaa tttattgttt cagtttggag 28200gctagtcatc tgaaaccaag gtgtcatcag ggccatattc tctctgaaac ctgtaaggaa 28260gaattctttc ctgcctgttc tagcttctag cattttccag caattcttgg cattctgttg 28320cttgtagatg catccctcca atctctgcct ctatcataac ataaccatct cctccctttg 28380tctgtctgtt cttctcatct tataagaaca ttaattgctg aactggggcc cagcatagat 28440taggcctaat ctcatcttga ataggttata tctgaaaaat tcttttttca aataagatca 28500catttatagg tactaagagt tagaacctca atatatcctt tggcggggac caaactcaac 28560ccataaaaag tattttatat gattccattt tatctcctcg ttaggttatt aactatatct 28620atatctatct atctatctat ctatcttttt tttttttttt aatttcagat ggaattttgc 28680tcttgtagcc caggctggag tgcaatggca cgatcttggt tcactgaaac ctctgcctcc 28740caggtttaag tgattgtcct gcctcagcct cccgagtagc tgggattaca ggcatgggcc 28800accgcgcctg gctacttttt gtatttttag tagaaacagc gtttcaccat gttggctagg 28860gcaggtctca aattcctgac ctcaggtgat ccacccacct cagcctccca aagtgctgag 28920agccgccaca cccagcctta taattctttt ttaattcagt ggttgcttta gggtttatag 28980tatacatctt tatcacagtc tagcgccaag tgatataccc ctttatgtat agtacacggc 29040ccttacaata gtgcatttcc atttttcctc tctggcattt aggccattgc acacacataa 29100actcaattga tccctttgtg tagatccgta tttccacctg ctatattttg cttctgtctg 29160aaatctatgt atgatttctt ttatgactat tttgagcaat ttggttatgt tcatttgcct 29220atttttctta tacactttta gctcatcaat cttaactctg tgagtttata gtttttaaaa 29280acaaatttga aattatttgg ctattatttc ctcaaatact tttttctgcc acccctgctt 29340tccctttcct tagggatctc cgatttccac ctatattagt ctgattgaag ttgttccaca 29400tctcttttga aaatctctgg aaaatctttt atcattggga taatctgtat ttgtacatct 29460tcaagtacat taatgtttaa tctgctgtta atcccatgca atggattctt catctcaggt 29520attgccgttg taatctctag aagttccatt gaagtcttac ttgatgaaaa atgtggataa 29580aaacaagtgg tggtaaagat atggagaaat cagaatccgc acacactgat gatgggaatg 29640taaaatggtc aaggcaattt ggaaagcagt ctggcagttt ctcaaaaggc taaacgtagt 29700taccatatga catagcaatt attcacctag gtatatactc cagagaaata aaaccttttc 29760cacaccaaaa cttgaacatt acttaatgtt cattaggggt ttaaaatttt gtacagtatt 29820tttatttaaa gtagagatgg gggtctcact atgttgcaca ggctggtctg gaactcctgg 29880gattaagcgt tgggacctgc cttggtcctg ttttctccgc tggaagagaa taaaactttg 29940tacgcttgga ccaacaactc ccgattccct cctttaccac catgccccat agcctctagt 30000aacacttact gtccacttcc atgaattcaa ctttttaaag attccacata taggtgagat 30060cacgcaatat ttttctgtgc ctggcttatt tcagttagca taatgtcctc cagattcata 30120agtgttatct caaacgacag gttttaccct cctttaaagg ctgactagtg ctccattgtg 30180tgtatgtatc acattttctt tatccattta tctgttgatg ggcacttaga ttatttcaat 30240gtcttggcta ctgtgagtaa tgcttcaata aatatgggaa tgaggatatc ccttcaacat 30300attgatttct gttcttttgg ataatactca gaagtgagat tactggatca tatggctgtt 30360ctattttttt tgagaaacct ccatactgtt tttcctaacg gctgtactaa tttacattcc 30420cactaacaat gcatgagttt gcttttctgt acatcctccc caacactcgt catctttcat 30480ctttttgata aaagccatta tataataggt gtgaagtgac atctcactgt ggttttgatt 30540tgcatttctc taataattag tgtgagcatt ttttttttta aatgtaccta ttatcatttg 30600aatgtctttt gagaaaggtc tcttcattcc tttgcccatt tcaaaatcag gtggttttct 30660tgctcttgag ttgtttgatt tccttatgta ttttggatat ttacccattt ccagatgtat 30720catttatatt tttttcccat tctttgagtt ctctcttcac tgtgttgttt ccattgctgt 30780gcaggtcttt tattttgatg ccacctcatt tctctatttt tgccctgctt ttgcagtcac 30840atccaaaaaa atcattccta agaccaagtt gtggagattt ccccgtatgt tttcttcagt 30900aggtgtacag ttttaggtct tatatgttaa gttttaaatc tattttgagt tttttttaat 30960atggtgtaaa caagggtcta atttaattct tctgcatgtg gatatccagt tttcccaaca 31020ccatttattg aagaacctgt cctttcccta ttgtgggttc ttggcgcttc tgtcaaaaat 31080cagttgacta taaatgtatg actttatttt tggattttct attctattct attggttgat 31140gtctgttttt atgccagcac catgctgttt aattataatt agtttatgtt tttaagtcaa 31200ggagtgtgat atattcagat ttgttctttt tgctcaaagt tgttttggct atatacggtc 31260tttttgtggg tccatatgaa tttaaaaatt gttttttaca tttttgaaaa aaaatgacat 31320tgtcatttca aagacattgc attacatctg tagattgcct tgggttgtat aaagatcttc 31380ctaataattc ttctaataca tgaacatggg atatctttcc atttatttgt tttctttcat 31440ttcttttatg ttttatagtt ttcagtatgc agatctttta cctccttaaa tttacacctc 31500agtatttaat agttgttgct attgtgagat tttcataatt tccttttcac atagctcatt 31560aatagtagat ggaaacacta ctgatttctg taagatgatt ttgtattacg caactttact 31620gaatttgtgt atcagttcta ccaggtttta gttttgttct ggtggagaca ttacagtttt 31680ctgtatatgg ttatgtcatc agtaattaca gatgatttaa cttcttcctt tcctattagg 31740atgcctttta tttctttctc ttgtctaact gctctggtta ggacttctag tactatgtca 31800aaaagtgatg agggtcagac atggcctcca tacctaatct gttgagagtt tttaccatga 31860gaccaggttg aattttgtca aatgcttttt cttcttccat tgaggtgatc atatgatttg 31920atttacaccc ttcattttgt tatgtggtgt atcacattta ttggtgtgca catgttgaac 31980catccttgca tcctaaggat aaatcccact tcatcatggt gaatcattct ttgtgttcgt 32040gaatccagtt tgctaatatt tgttgaggat ttttgcatcc atgttcatca gggatattac 32100tttgtaagtt tctgtccttg aagtgtcttt gtctggcttt aataacagtg caacactact 32160cttgtaaaat aagtttagaa atattccctc tgcttcattg ttttggaaaa gtttgagaat 32220ttgtattagt tctataaatg tctggtaaaa ttcagtagtg aagctgccta atcttgggct 32280ttcctttggt gggatgtttt tattactggc ttaatctctt ttcttattat tggcttattc 32340agatcttgtt tcttcgtgat tcactatttg taggttgtat ataactagga atttattcct 32400ttctttaggg tagtgcataa cttcatagta gtttcttata atcctttgta ttttggtgat 32460atcagttgta aatgtcccct ctttcacttc tgatcttatt tgagtactct cttttttccc 32520tagtctaggt aagaatttgt ttttatcttc cccatccccc ccccaaaaaa accccacaat 32580ttttagtttt gttcattttt tctagtcttt atttcaactg tgatctttgt tatttacttc 32640tttatgctaa cttttgggct tagtctgttc tttttttagt tcctttaggt gaaaagtaag 32700attatttgtg atccttcttt gtcgatgtag gtgtgtattg ctgtaaattt ccattaggac 32760tgattttgct gcatcacata agttttgttt ccattttcat ttgtctcaag gtaatttttt 32820atttactttt tgatttcttc tgtgaactat tagttgtttg ggagcatgtt gtttaatttc 32880cacatattgc tgtattttcc accagaattg attcttgttc ttgatttcta gtttcatgcc 32940attgtaatca gaaaagggac ttgatatgat ttctgtcctc ttaaacctaa gaatagtttt 33000tggactaaca tataatatat cctggagaat gttccatgtg catttgagaa caaaatgtat 33060tctactgctt ctggatggaa tgtttcatat atgcctgtta ggtccatttg gtctaaagtg 33120taattgaagc ccattgtttc ttaatattga ttttctgtct agggaatcaa tctgcccact 33180gtgaaaagta gagtattgag gtcctatctc cctcttcaga tcatttaaca attgctttat 33240gtattcaggt gtgtcaatgt tgggtgcata tatttttaca attgttatgt cttcttggtg 33300aattaatccc ttatcattat ataataaact ttatttcttt ttatagtatt gacttaaagt 33360ctattttgtc tgatagaagt atagttaccc ctgctctcaa tttccattta ggtagaatat 33420ctttttccat cccttcacct tcagtctatg tgtatgttta gtagaaaagt gaatctcttg 33480ccgggcacag tggctcacgc ctataatccc agcactttgg gaggtcaacg cgggtgggat 33540caccagagat cgggagttcc agaccagcct gaccaacatg gagaaaaccc gtgtctacta 33600aaagtacaaa attagctggg tgtggttgtg ggtgcttgta atcccaatta cttgggaggc 33660tgaggcagga gaattgcttg aacccgggag gcggaggttg cagtgagaca agattgtgcc 33720attacactcc agcctgggca acaagagcaa aagtctgtct caaaaagaaa aaaaagaaaa 33780aaaaaaaaaa ggaaaagaaa aaaaaagtga gtctcttata ggtagtatgt ggttgtgatt 33840taaaaaaaaa aaaaaatcca ttctgtcatt ctatgtcttt ttcttggaga atctaatccg 33900tttacattca aggtaactat tggtaagaaa tggctagtgt cattttgtaa tttgttttct 33960gattgttttc tagctccctt gtttcttttt tccctactgc taccttcctt tgtggtttgg 34020tggttttctg tggtggtgtg ctttgaattc tttcttttat agtctatgca actaccgttg 34080tatttgccat aaggctaact taaaacattt tatccttata acaggatact ttaagcagat 34140aaaaacttgc ctttagttgc atacaaaaac tctatgcttt cacaaccccc caccttttat 34200gattttgatg tcaaagttta catttgtttt ttaaaatttg tatctcttga caatttattg 34260tagctacagc tgtttttgtg tcatctttta aacctaatac aaataacatt gctttacctg 34320ccacttttac catagtagag aattctatga atgacttata ccatttagtt ttttactttt 34380atgtttttcg ttaactagca gtcttttatt taagcctaaa gaactccctt tcagtaattt 34440cggtagagca ggcctagtgg tgacaaactc ccttaggcat tgtttatctg gaaaagtgtt 34500tatttctccc tttgccaagt aaagtgttgt taattaacag ttttgtttct ttcagcactt 34560tgaatatacc atcccactct cactggcctg taagactttt tgctgataaa gccactgaat 34620ctgtattggg gctaccttga atgtgatatg tttcttatct ttgctgcttt cagtattctt 34680tgtctttgat ttttgataat ttggttgtga tgtgttttgg tgaactcttt gggttgaatt 34740tggtgacctc agcttcctgt tactgaattt tgtcatcttt tctcagattt gggaattttt 34800cagctattcc ttccttatat atgctttcta ggcctttttc tttcttttct cgttaaggac 34860ctcctattat gcaaaagtta gctagcctga tgtgtcctat aattcttaca ggcattcttt 34920tttgtttgtt tctcattgga taatttcaaa tgtcttacct ttgagctcac tgattcttct 34980gcttgatcaa gtctgctgtt aaagtgttct actgagattt tcagttcagt tactgtatcc 35040tttatcttta gaatttctat tgtttgtgtt tatatttctt tgttaaactt ctcattctgt 35100tcatacgttg ttttccaaat ttcatttttc tgtccatatt ttcttgtact ttgttgaact 35160ttaagaggat tagtctacat tatttgtcat ttcacagatc tctatttctt ctgagtctgt 35220tactggagct tcgatatcca atgtacacca gaaactttgc taattccata gtaaatgtta 35280gaaatgggtt ttttaatggt aaaatcagtg gcttgctaat ttgtatatta gaagctggct 35340tgcattggtg aagttgaggg cattcgatat atgtatcctg gctaaaatac ttctagagaa 35400cctctccacg ttgcaagaat agggtataga aatctgagga ggcctttaac atggccctca 35460gaaaggacac acagggcttg acccctaaac atataaacat gtacagaaat gaggtgagaa 35520catgttttga ctgatgccag tagttaacac agcagagcct aatgctggtc actgttacaa 35580ataccttgta taataaacct ttaatttgag aggctgtaag ccctgaaagc aaagggctta 35640tagtacctct tgcatgaaca ctcctgtgtg tgctttctaa gaaatgcttt aaaaataatg 35700catgtggcac aacttgattt gacactggct ctttataagc tgctatcaag gttgtgggga 35760atagctaact cctacccact ttccctgaca aatagaaact ggtagttacc taccaaaagt 35820atgtaaaagc tgtatctgac ccaaaggcca gttaggtcaa aacaccttga aaggagctca 35880gtttaaggaa atcagtggtg tcacagagcc ccactaacgt gtgtacaagt tttcatagtt 35940tgactatagg atgtgcttag tttcacaggt gagtcttacc ttgtgatact tttagagcaa 36000aagtcaaatc aaagatgatt ttaaaaaaac atttcaaaga atcataaaga ataaatgcct 36060ccttaggaag tcttctggag ttctgcccac ttcccttagg tggctcttta ctgtgcccat 36120ctcatagctt ctctccctgc atttggtgta ttgcagagta aagctcactg tttacaggag 36180tggtagaaag tgtggtcctt tcccggactc ctttttccct tccccttctc gtttctggga 36240atatgtgttt gataatagaa ttaaacaaca gactgacaaa tgactaacac atttagctaa 36300gctacataag cagacgtcag tttgacgtga agggtttaaa atatctatga attctctggg 36360gactcctccc ccagacctga aggatcaggt gctgccttct atgccacctg tgcagagggc 36420aaaagaggaa aaccatactc atgttcagtg tgagtaaagg ggacatttga actctgtgca 36480gacccctcat tggaagggtg tttcttctcc tgctgcatcc acaaagagca ctccttagcc 36540ttgccttttg tcagttctct ccccaataag gttctgagca gagaacccag tgcagttcag 36600agactgaagt ctggtgtccc aggtctgagt tctagctcta gctctctgtg taactttggc 36660atgtcccaaa gtaactttcc acaacttgac aggtgttact tgagttttgg atacaggtga 36720ctcttagccc atctgtgcct cagagatgtc atcacttggg ccacatgacc tctggacacc 36780tttcctactt tgagcggcag aacagatggg agctcctgct gcctctgagc ttcagtgaat 36840tatcacttgt tggagggaag cttcaagcat tttgtatctt ttgtatcttt caagagcaag 36900cacagtcagc aagaatatgt agcagatgct agtgaacacc agtgattagg gttgaatgct 36960ggatttaaat atggagctta ggctgtgaag gaagcctgaa gaacctagag ccccacaaat 37020tgctctctgt gagatctgac accatttccc actgatgagc cttgggtcta ataggtagca 37080ctgattccac atagagtatg tgagtgtaat acactgaaag caaagagaat aaaatgatgg 37140ctaacatgat gttccaaact ttaaacagga gaaaaacaca taattccatt atgtataaga 37200acccacacag agatcaggag aataacctca ttggagaatc aatgacctat gtggggaatt 37260tagggtagag ctgagattga aaaaatgggc caaagccagg tggccaaggg ccttaatacc 37320ttgtatacaa gatgtgtagt caagaaagac catgccttag ttacgcagca attcccttgg 37380gcctacaaag agctgcctag cctgggacta ttgtagagaa aggctactat gttccagtga 37440tgcagggact cctccatata tatatgcgtg ctcagttggc tctgtaatac atcttacttt 37500tatttattaa cttttcttgc acattggctt ggtgcatcag ttggaagtca gaggctgaac 37560gaagtgaaga ttgagccaaa ggaagttctc agcctttagg gaggtggaat tcacattaaa 37620cacacaataa acaaatgaac tcacattata aaggagagag tcagaagatc ctagtggctg 37680gtgtcatcgg gccttatttg cccgaagtgc ctattcttta taggaatcca ctcccagggt 37740agatgggcta catccttagt aggctttatg cctatgttct cctggccatt gcctcctcca 37800gggctggcct ttttcagtct ctctgtagag gttcctgtgg ctggttggat ataggccttc 37860acagaagggt cagtgccttg gctcaggtta caggactggt aatcttgctt tgttaagagt 37920aatgttattt ccccatttcc aaattctcca gggagatgga atgtcaaaga tagtatcact 37980gtagcaccta catcctaggt tccagaagca gagaagagaa tatactgaag ctgtagaaag 38040gcctattagt ctgtgtcaga gattaactgg atgtgaggac catttctggg atggtgtata 38100cagaactgga gaactggata gggagtcaga aagagctgaa gacgatgtct ttgaagtttc 38160caaagtggat aactaggtca acagtgtatt attcaagaaa gtattaacat aggattgaga 38220tgcagtcagt agtcatggaa ttgaaatttc aaagtatata cctcttggat ctcaggggtg 38280ttgagctgat cacctgggct aacactccta tgaccctagg gaaaatcaaa cagcctggtc 38340ctgtggccat ggtaggggtg tcatcacctt aatcaaactg ggacagtgct gttggatatt 38400catctggaac ttgttgcaga cccacatttt gctgggtttc accacaacca aggccttttt 38460gatccttttc tcggttaaca tcagtacatc attgcaaagt taatcctcat ataataggga 38520atgaaactaa ttgcttataa aaacaagatt tttacaactc taaaattgtt caagcatacg 38580ggcatattta tagttgcagg cagtgtttca ggtgcagact gttcttggct gcagtggtgg 38640tttacagaca gcatctgttc aatatttgat gattatcccc gaatgtttta aagcgtagta 38700ctgggctctg ctgaccgtac aacaaactgg catttttgac ctatagggcc ctgggctagg 38760agatacagtt ctgaggggaa gtgaaagaca gtgaacaact gcacaactga ccctttgtta 38820gttgcaataa agcaatccaa ccacccaacc cactctgatg gctttcctac tatttaaggt 38880tggtgatgtc aaagagacac cctcctgttc agtgtgcagt gagtcaacat catttccaca 38940aaacctcctt cctgcacaaa ggacttatca tagttggtta agaagtaaaa ttttcctgta 39000tcagtcacag gagttcacca gttaagatac tgttagctga agacttctgg ggtgacttaa 39060tgaaatagct cagccatgtg gtttaaaaac gagattcttc catccctcca cacagctgtc 39120catgcatctg catcatgtca aggctggtct ccttggtggt agaaaggctg acagtaaaaa 39180ctggagccac atgattcctt gcttaggcac agtgtttctt catactctca catgggagca 39240ggcatgttct ttccccaggc tcacagtaaa cactctgtca aatttcacag gcccaaagtg 39300cttttggtta tccccattcc aaaccaattt gtggcaggaa agcattaccc tgactgcctt 39360agactaatat acctactgca

cttctagggc tggggataat tttgaggtca accattcaaa 39420ctgcacggca gtcattccat ggaagaagta tggtctaaat ctttggggca acctgaattc 39480atgaaaactc tcttagattt atgtaactaa ttttagaatt cacttctgta ccatttaatt 39540ttactaataa aaatactacc tttaccctaa atttcagcca agcgtaaggc tcccttggga 39600cagaaggaat tatcaaagct ttgtgttttt atacatagta gcgtttgaca aagaaataac 39660tctgaaggaa ggagaacaac caagcagagt ctagatgcac agaagtcttt gaggcttggt 39720aggcagaagg aaagggcagg actacttcag gaatacaatg tttcagtaac agaggttggg 39780gtctgttgat cttgaggaga gatgaggatg gactggaaaa taggagtgag atagtaggag 39840aggaaaggag atggatggtg tatatactgt atggctaacc atcaatcgac cctaagaaga 39900taatagttgt tagatggtta gctatttaaa gaaagaaacc tgaacaagta acaatcttga 39960gttgcaaagt ggctggagtc acacaacaga ctaaatttct ggtagaacaa atgcagcagc 40020ttatgtgaag attaccatgt ctgaagctgg ttagaaaagg tctaacatcc aaccaaagtc 40080acagttcttg agctcggtac acagagacag actgctaaca gctgatgtgt cctcagcgag 40140agtgtcctca tttatatcct cattctcctc ttctgcctcc ttttttgttt gtttgtttta 40200aacctttgga aagtctcatc attcaataca gtctaatata tcacaataac agaggcggcc 40260caatttctac agattgacta attccatcct tgaaaagttc atataataaa actatataaa 40320gcatcatttt caaccatcct ttagaaaaat tcccctatta aatgttaact ctaaatcctt 40380tatctctgtt aataacctta ctaaaaactt ccccatcact gcctgccagg gagatcaaaa 40440gaaatcacat ttacgaaacc tccaacacct gtacctgact gaaaagcaaa catacagacc 40500tttcagtcct gccctactat tccgctccta tcacacagag gcacgtggac atctccttgg 40560gaagcggaca gctcttaggt ggaagctagg cctgcagtaa gcaggccagg aaacatgtct 40620cccaggaccc acactcttgg gagaagcacc tggccaccag acagcatgtc ctgtcaacct 40680acagagttct gaaaaacaaa cactggaccg ggaatagaac cctgtggtca cagtgcccac 40740agttcactaa gcaccctcac aggtctttga cagaacactg actgccaggt cccctcgtgg 40800gcagagaaat ggaagattcc caggcccaac tgagcatctc agggaagaac cacaagcaga 40860gcaactgcca gagctggtcg gctagcgttg gcaaccaggg aagcaagtgc tacaccatat 40920ttggagagag cataaaactt aaggaaatag agaagtccta ctcaattctg ttcttcaact 40980ggaaacgaga acttgcgaaa aagaaaccca gttttctgga gtccatggag aaatatgaag 41040ccaatgctcg actaacagag cagaaagcct tttataaata atgccagtca aagctccaaa 41100ggtcagagct gatgcaagcc tgactgttct gacaatttcc taggttgcta ggcaacaagg 41160agctggtcaa acagctcacc ttcgaggacg tactttataa taccaccctg gtagacgagc 41220gcgaggcagc agtgaaggca gtagtcaaga acaggagagg gaggttaaca gcaagatgct 41280cttcagacag tctggacaaa gacagtctct acccagctct caggagtggc ctgcttaaga 41340aggtggaggc ccaggcagct cccagggtga ccctaaagac agactccaag gaaaagggtg 41400tgagggcaat ggtgaatagg aggaggtccc agtagacaca gcaagtcagc tcaggtttta 41460tctttagagt ctctgaacta aagataagtg aagagaagcc ttctgtggac agcattccag 41520cactatggga ggggaaaggt gccacgagag acttctccag tggggcacac aagcattgtg 41580cagtgatcct caagatccag ccattggcag gaaatcgaaa ggcaagcttc ttaattacat 41640agtctatgga gacagaagtg tggtgcaccc ccaaaataaa aggccaagac ccaggaacaa 41700cctccacaac ctggagtata tacaaatgaa cccaggcctg ctgactcaga tccacgccat 41760cctaaagcag ggtattctca cgaatgaaag ggctgagtat ttggtgtaga gaatcagaag 41820tctcccactg cccacacatt ttctcttcaa agatttcttt tccaagaacc cattgaataa 41880agggagggag ggagcactga atgccccaga catcagaatg catgatgcag gaagaggaca 41940gtaaatagtt ctcaaagaga tcagaaaata aattggaagc aatttcatta ccacaaataa 42000tagacctttt ttctaaggca ccccttccct ttccacccaa ttgtcattca gcaactactg 42060aatacttacc aatgaaaaac cttacccctg acctcaaggc tgctcctgag ctctggtaga 42120aaatgctttc ctcatctata aaacatggca agcaaggcag gatttaatag taagaaagag 42180aactcagggc acagtagcac tgcaggactt caaatggaaa tatggagaca agcagtgaga 42240acaggcagca aaagcagggc aggtgaggct gggaccaggg catctggaac tttccacacc 42300agcttggatc tccatgccaa acaacagttt tcaaggaaag gtatctaaga ggaacatgac 42360tctgggaaac tttttggcag tattgcttac tgcatactag aaagtaaaag aatttgggga 42420acattcacta atttgcttct tcaggggctt gggtagggaa caggaacagg aacctggctc 42480taatttctga acttttatca gtaaaaacaa tccaacaaat gaaagttagt caatgagaga 42540actgggaggg tctgccctcc ctcctccttc cttgagtcaa gccttcttgg gggggacctc 42600ctgacattta attaagtgaa ggcaatgtcc actgaaggaa gcggacctga aagtgacacg 42660ctactgtgaa atgagcatgg agtaggagct tgttacatat atgaaatggc cagtgctctt 42720gagcaaagcg cttcacaact tgagacctaa gttttctcct ccatatgctg agggctgggc 42780aagatgatct gtcaggccat ttttacccct aagccaccaa aatccaattg ctttagcttg 42840tcacaaaagc aggtatcgaa tggctatcct gcagtccctc cagtcaacat ccagactttt 42900tccctgaggc aatataagac aacagttaac atatttttat cagtttaggt ggtcatgaga 42960taaacatatg ggaggtggta gtttttcact taaatgaata taataatggt acagatctct 43020ttgagtagta tttgtttctt aaatatttaa gttccctaaa gacggtaaga ataacccaag 43080aaagtgaaat taatgtcaca aagcacatgg ctaaataact gcaggtttgc agtgccatgt 43140gtgagatcag atgacagaag ggaggcctac cttcaggcag aggcttctca tgttccctgg 43200agtggccatg tgctcttcta cacgactgct tccacttcgg ttacgtagaa gctatttaaa 43260gcacacagat ggttgtgatg agataaaagc cacccttaag tgaataatgg aaattaaagc 43320atgatttgag ggtgggggtg gaggtgggag tagagatggg tagaaaggag tgcaagggaa 43380acaaaggagc cttcataaca ttaaagtcac ttaggataac atgattgatt tactcaacac 43440cttcttagga acataaagca aacaagtggg ttttcctttt actgcttttc tgaaatgagc 43500tacactcaag aaagcaacat gggggttgtg ctgtccctgc acagtggcag gagaatatga 43560ggagcagctg aatgccagaa cagctccatc caagatcatt tttcacacgc atgaaccatt 43620cttatactac cttttactgg caatttctgt agaaatctgg aagtctggtt gacaaatcag 43680gcccttgaac tttgcaatca cggtggcatt catatattca ttcagcaagt atgtgtgtac 43740gggacagtgg agcaaataaa acagatacgt tcctactgtc atgaggctta acatttaagg 43800agggaattag gcagtcatga agtaaacata aaaatacaga ttgtaatagg cactatggaa 43860taatgaataa tttggggaac acaatttaaa tttgaaatat atctcagttc cattaacaca 43920tgttgaaggt gaatggcatg gcactttttt gctggattta gggcctggat gcaagcatat 43980tggggtaact tctgaaatgc atcgtagggt aatgcatttc ctcccatttg ggcccaagtg 44040tattatttac cacccagttg tgatgagctg ggatcctcct gctcaatctc agcttgaagc 44100acttggaggt tacctgcctg ctgtgggtga ttattttgga gcaaggtact tcatttgcct 44160caagaaacag atttgatacc actactgtcc ccttttggaa cagagaagta ggcaagaccc 44220cagtgtgagg cagagtgatg ggatctttag gatgccagag tcattacatc attatgtaat 44280gtatgtaatt gatgtaactg atgattttgg agtttatata tttccaaagt ttcaaaatat 44340tttcacttgg ctgatttatc tttatggtga tgacagtaag tagatatatg ccctccttaa 44400aagttacagt gagagttgac attgggaaaa gggaggcttg gccagggtta tgcagacaca 44460gtacggagga ggatggaaag tttctgagac accccagtta actgcgtgga tccacaaagc 44520acatgtacag tctgctctgt gcactggagg tacagccacg aaaaagacag tctctgccct 44580catggagctt ggtgtctgtc tactgtagca gacaaaaaca aacctgctgt ttctcatttg 44640cctctgctgt gggttccctg tgtaactctc aaatgctagc atttccatgg atccttgtcc 44700ttctctcctc tctgcctttt ccaggatgag aattttcctg gccacgggtt cagctatggt 44760ctatgtgcta gagtcataca tttttatttc tgtgcacatt tttctctaga cccgtatttt 44820taactgcctc aggacagctc tccctcgata tccctcagac acaactcaaa cgtcattcaa 44880ttctactatt aatacttttc ccttcaaaac ccactcttct tctgtagacc caacagcaac 44940actgtccacc cagctctcaa aacctgaaat gggggagctg tctttgactc aactctccca 45000tgcatgagca ctgaatcaat caccgagtcc ctagcatgtc ttggactctg gcctcccttc 45060tatcctcaga ttgtctgcct tttaaaaatt atagcaataa cttaactgca aagaccacat 45120gtacacttgt gctctttcag tttgcaataa acataaaatg taaaactgat tgtctttgct 45180tcttagcatc tgcaaaataa agtccaaagt ccttggtatg gtctgtaagg ccctcattat 45240cccaatctca tcttgattcc ttacagcctg cactcagata tactaaagag cgtccgcctg 45300tctgcctcac acacacctat cctgtctgtc aagattcggc ttgaccttca tcacctatct 45360gcagtatttt tggatttgaa ttctctgccc acattgtact ttcacatact tcttttatcc 45420ttagtggttt gtacttatgc ctgctctaac tagactgtct ccctgtagta gactccttga 45480ttcaacaaag cagctctcca tcagcctgaa atccgtggca cccggcaaaa tggcaaacat 45540gcaataggta tttgctcagt aaatgttaat gaaaggaaaa aaattcaaac ttcagttgga 45600ataggattag agacaactta aaaagtttcc catagaaatc ttcctcatca taaatatcat 45660catcctcaat gtcccgagtc tttccacagg tctgatttac caaatgagga agactctttc 45720tcactgtgtg tgtgggagag gcaggcgcag aacatgtttt tttttctgca agtctgctct 45780ctggattcat ttttctgggg atgaaatctc aggctgtgta gcttttctgg tccctttttg 45840gtaatcaaca aatcatcagt tgttctaggg tattaaaaag cccacacctt tgaaacatca 45900agatgccaaa ctctctctta atggaaaaca taattccgcc tcttactgag gcctttgagt 45960gggaagctta aagacagcag tgtggggaaa tcggtttttc ttttcctccc tccaccttct 46020ttcccattca agagctgctg ctgccctttc tggaggggag ggaattagaa gaaaagatcc 46080tgccttaccc agtcactgtt aattattact ctgagccagg gtggtgaatg actctttcct 46140ggaatacacg ctgctgggaa ccaccgctga gattctctaa gcaggcagct tccaacctct 46200cctccctcaa cagcttcaac attatatggc actcagctgc agggtgcctg gctccaggca 46260ggcagcccac ttgtgcaggg tagttttcca gatggctcat agtccatctc ttttcagtga 46320ccagtgtggc ccctgggaaa catacgtctt gactccatta ttggtgggag tactctttag 46380ttagttgcca caggccactt cagacatgtc ttaccctgag acccttttca gagtcaggta 46440ccatccctct gtctcctagc atctaaagat cacagctgaa ggtctccaag tagttcactt 46500aaaaaatacc tcagtaggtg taagaacagg gaaagccccc aattcattct gagagctgta 46560tgactctcat ccccaaacca gacagatata taatatgaaa actacagacc aatattcctt 46620atgaatatac atgcaaacat tctaaacaaa atactagcaa accaaatcta gcagcagaga 46680acaaggttta tgtagcatgg acaactgaga ttatcccagg aatgcaaggt taattcaata 46740tacaaaagtc cattaataca acatattaat agaataaagg acaaaacaac atgacaatct 46800tgatacaaaa gaagcatctg acaaaatcca tagcccctct tttttttttt ttttaattaa 46860aaaacagtaa actaggttta atagaagaat ttcctcaact tgatgcaaac ccacagctaa 46920catcatactc aatgctgaaa gactgaatgc tttcccccta aatcaggaac aagaaaaaga 46980tgtctgctct tgtcacttct atccaatact gtactggagg tgccagccag tacactaaga 47040caagaaaatg aaataataag aatctagact ggaaaggaag aaggtatttc tgttcacaga 47100tgcatgatct cataagcaaa aaatactaag tgaccaaaaa aatagaattt tagaaaagct 47160acaatacaca tgatcaacat acaaaaatca attttattac taagaccagt gatgaataca 47220aaaataaaaa ttagaaaata atcatataaa gtggcatcaa taaaatactt agaaaaaaat 47280taaagaagtc aagacttgta cactgaaatc tataaaacat cactgaaata aactaaagac 47340ctaagcaaat gcaaagacat cccacagtca tggaatagaa aacctaatac cattaaaata 47400gcagttattt ctcaaatcaa ttcaactaaa tccctatcaa gatcccagct ttgcaggaat 47460tgacacaatg atcctaaaat tcgtatgaaa tgcaagggac tgaaaataga caaatgattt 47520tggggaaaaa aaaactggca gacttgcaca tcccaaatcc aaacttagta caaagctaca 47580gcaatcaaga cagtgcggtg ctggtgtaag gatagacata tatgacaaca gagttaagac 47640tgagaatcca gaaagtctta catttatggt caactgttct ttcacaaggg tcccaggacc 47700attcatgggg aaattaaaat agtcttttca acaactggtg cttgggcaga tggatatata 47760gataccaatg cacttatgaa aaaaatggat gaaataggaa gcttcactac attctactgc 47820atgctgggtc attttcaatc atttaggtgg caacactgac aagataacag aaaaatggag 47880gcagtaacat gtgaaaggca aaatggttgt tgttttttaa aaaagggaca atatccatca 47940tgaatttact tacactccag gcaaaggtta ttaaaagaaa aaaaaaattc cttaactgaa 48000atgtggaagg ggtatcaaga gcagacccta caagaattcc agtgactcct cactgttcaa 48060ctaagagatg taccccacca tgccgtgcta ccatggaagc attggaggca ctctgggggt 48120tgatgacgtc ttcatggatg aggtacttta aaatgtggtc atgaagagta tttgagaaat 48180atgacaagat gctgggtagg aatgcaggga gaaaagtgtg taacccacgg ccaaattgga 48240aagatcagtg agggggcaat ttgtggaggc actgaacgca gagaatatta aaattagcaa 48300gatggtgttc tagcacaatg aaaaggattg gaagagggag atcagagtca gggaattaaa 48360ttaggcagct gctaagaatg tccaggtgag tcagaggatc aggacctgta agggcagtat 48420aaataaagga ggaatgacag gagggggttt acggggaaag aattgacaac ctgtgacaat 48480tgaggaatga gagattttga tgattggggt gaaggttaca tttcgtaaaa agaaacaaag 48540aatggtcgac agagacgcaa gatgaagaat tttgttttta ggcctactga ctttgaggta 48600gcgggaagaa atcctggaga cagaggtctg agtagaagat acagaattgg ggaagaatct 48660ttgggagtgc ctgaggttga ccagagagaa ctgggcaaga acaggtaagg actttaagca 48720gaactggagg agtatccatc caaaatctgg gtaaattggg aaggaaaaat aagcagccag 48780agaagcaaca gctcaagtta gggtacagtc aaggttatag atacgtgtta ctgattttgg 48840caatgaggag gttataagga tccttgaaga ctgtactttc agtgaaactg tcatggatta 48900gtagcctttc acagtgatat ctaccccaca cctgcatcga aatcttgcag tggctgttaa 48960caaatgccaa cccactaaca gggaggggaa aaaaagactt gggactctgt acttgtaaaa 49020ccctctaccc tccccaggca attctttaca cactaacact gttgaggtaa aaggaggcaa 49080ccaaatgagg gagcaaataa aatggaaggt gtgttttgga ttatacagtg gcccatggaa 49140gtgtgggagg ggtacagatg gcccttagac agacactggt ggaaagtcag aaaaaaaaat 49200tgcggtaagc aggtaaaggg atcaagagta cacaggaact ggtcttggga cgacatttct 49260ttctctgaca gtttgtatgg aattcttggg gaaattcctc aaaggggcct ggaatctcag 49320aagggtcatg tttttaatgg gaacagggag tgatgctgcc ccattacaac catctaacag 49380aatgtctgta ccgaggaggg aggaataaca gcaaggtctg tttgaagcct cattttcaag 49440tttctttttg atatggatct ccctatacaa gcaagcatcc ccaaaagtag ttatctcagg 49500aaaccaaggt taggatgacc agctcatttg ctggagctgc caaggtccag aaaagtttgg 49560ctgcaggctg ttttcatgtt ttatgtttga aatgttctac aatgaaagtt aaaaaaagtt 49620agcatttatt tggaaaacca gttactttag tttatggttc ctttttttcc ctccagagct 49680tcctggagat tgaggtctaa ttcaaagaaa accaaactat acaatagagt acctgggcaa 49740aaaagtactt ttataacata acatttgggg tagaggaagt atccactatc gtcaaaactt 49800ctatgttttc ctcttcctta ttgttcaggg acattccatg cagcaaaagt aaggggagtg 49860acaacatgcc tagcataatt aagcaagcta gagcagctag tctgtgcaac tgatgatttt 49920ttccctctaa aattttaagg gtaggttcat tgtgactctg ttagaagtct acttgatgtg 49980aattctgtat ctgataacct atttcaatta tcactttaaa atttgtcata tggatacatt 50040attacaatca tagaacttca gtaccgtaat aataaaactt gagaactgaa gagcacacat 50100ttcttcacga atttattata taaaacgccc tcagagtatt taatttctcc tcactttaat 50160tacacattaa gaagcacagt ggatgagaag cctttaagat gactacagtt gcacgaaggt 50220ccctttcatc aaggtagcgt acgtacctta acagtgttct aaaggctggc ccagaaaaac 50280cccatgttac cttatcacaa tatggaaagc attgtcttct ttttccacta aattatggtg 50340aaaagtgcca cagttttatt tagcattatg gtacataaca gttctgtctc aattatgaaa 50400aaattaaaat aatcctgaaa gacatccttt ttctcccccc aatgatttga aagctgcatt 50460tttcctgcca atttcaaaca aatcatcagg ttgatctaca gtaatcagtt aaaacaatca 50520ccaaggcaca agctcagcac attagctata gcttgtagca aaaggatata tcaatgtctc 50580accttagtta aaaatacata atccttttat tttataatgc aataaaagaa attaacatca 50640catacacaga agactaggaa aggggaaact acttacttcc gaaaatccag taatgtaaac 50700ctacttgtac ttttccatag tacatgaaag taatgtttaa catgttttga attaaattta 50760acctgtgggg ctatacaatg taattcttag gagtaatagt ttcattcatt tccaggtcag 50820cttactgtat gattaagtaa cacaaggcac agtagccatc tttttcatta tgttgcaaca 50880ctgatcacgt gcctcgataa aatggctgat tcaacaagat gatggcaaca cgaaggggag 50940actttggatt gtctatttaa aatctaggta ataagtaatt aataaaaact ctatcttaag 51000tgcactttca catgcttttt gtttataata aacaacaaac ttcctaactt tgttgcaata 51060ggcttgacta ccatttcatt tggccaaatg cactttcccc agtaaactta aaataacaag 51120aacaacaaga acaaaaatcc ctgtcctttc atatactaag aaagaggatt ggctactgaa 51180acagttcatt gcaagacaca tgaagacaac atactgtggc atgagttgtt tttttaattt 51240gttgtgctgt tactaaagtt ctgagggctg cagttaaaac attccaattt ctcccttcca 51300tctttattga ttctcaagat tttgcacaga aaactctttg ggggctagaa cagcagtaat 51360tgcatcacac tgtttcaaag acttcaagtt tcaaaagcaa atcattaaaa aaaaatacag 51420ttcctgattt gagttagata cagggacaaa aaagtagcac atacttgaag gttacatggt 51480ctacaaatgg tggcaatatt tttcttggga gagtagttct gttggtatat attttttaaa 51540tactcaaaag gctcaacctc aagcagtaat aaacacaagc aaaagtgatt taacccttaa 51600aataaatatt cagaaaaacc tctctgtaca tacaagtgaa agaatatgta acactttcac 51660gcaaaaaaat aattataata ataataaagg atttgttcat atatgtagct gaaatctgct 51720gttccagccc acatgtcccc aagaaagaag ggaggcacag acagaggtgt gactactgtg 51780gttcactatc ttacagcctt tttgtactgg gacactatca ccaccaaaca tttatccctc 51840ttgttatatt tttaaaattt tttaaatttt ttcttttttt tttcttcctt ttttttgttt 51900tattttgttt tgttttacag catgccaaat cctttggcat acgtgatggc cttcaacaat 51960ctctctttaa gtttttcttt gcttgaatat tccggaagta aaagcacatt aaagcaagta 52020tgagatgtag gtaacctaaa tagagaaaag gggaaaaaac aggaaaataa taagtcatgg 52080gaaatacact tagaattata tatagttgag atggtttgca atgccaacta tacattaatg 52140caaatcataa actttttgtt gtgtaactac caagaatcag tttgtcttta tcctataaat 52200tactcaaagc tagtaacaat gatgataaga tacttatcca ttgagttttt actacatacc 52260agataccgtt ttaggtattt aattattaca gttcatttca ctaaatcttt ttccacaaca 52320aaaccacaga gaggacatca ataaatgcac ttcagaggtt aggtcactga gaagtaactt 52380cctctaaggt ggagaaaata ttcaaacctg gtttacaaca tttcaaactg tgtgctctta 52440aatgcttatt agaaatactg ctggcaatat gactatgaaa atgataacag gattctagaa 52500taatcgaatg ataatcttcc aagcctcaat gtaaccattc taaatagatt atcatgtaac 52560atggctttca actaacaagc tgggaatcaa aaaagtaaat gaatcatact aatttgaaaa 52620attccaaacc aatgtgagtc ccataataat ttttaactag ggcaattcct taaaagtttc 52680ctcacacaat gacagcaaaa tattttctca attgtctaat atgatttgag gatttgtaca 52740taaaatcaca aaggtgctta gaaactataa agacagttca tatgtgtgtg atgaggaatg 52800caaggttttc agtaggtata gagtcaccac ttaatcatta cctttctgtg tctgggccat 52860ttttggctat aatcatcttt aattttccta gtcctcccac aggtgctctg tctgtgcccg 52920ttgtaaactg caagaagagt cttttctgtt catctgtaaa tgaatgaacg atttcccaga 52980actccctaat gagaaaaaat acaatactgg tttcagtttg gcattcatta tgactggtac 53040taatataagc ttatgatttg catttaaact atattaagag acaacttgga agttaattat 53100caggataata tcacttctgg tgatttaata atttccaagc aaatttatct taaacagttc 53160tgaatacata aaaatttaga ttacaatata gtaaaatatt agtactacaa tagtaaaaaa 53220ttgagaaaac ccaagtgtgt aataacagga aatgactatg taaactatgg tataatcaca 53280ttatgtagag tcagtaacaa acaaaagaca aaatcctatg aattgaaaaa gactgaaagg 53340aatattggga aagattaact ccaggtgcct ttgggtatat attttattta tcctttctcc 53400attattctcc ttgtctactc atcctttaac tcagctttgg gggggaaaag tgatacagat 53460taaggacaaa agaaaaaaaa agaccccctg ccccaaccaa ctggccccaa atgcaaacaa 53520ctatatacat acctaatagc agtaatacaa aagttcaatt tttataggaa ctagaatact 53580tttaacaagt aatatgtgct gtgtatggag gaggagaatt attctgacat ttctgccacg 53640tgcagttatt ttaagagaat gatttatcct ctggcattcc taaaatctat gtaataaaag 53700ctatgtttta tgacacttac gttagtttga gttctaaaaa acgaaataca agctcataaa 53760gtgcaatctt gaagtttgtt tgaataatca ggcatctata aaacatatat acactagttc 53820atagttaaat aatttttttt tttttttttt tttttttttt aagacagagt ctcactctgt 53880cacccaggct ggagtgcagt ggtgcgatct cagctcatgc aagctccgcc tcctgggttc 53940agaccattct cctacctcag cctcccaagt agctgggact acaggtgccc gccaccacac 54000ctggctaatt tttttgtact tttagtagag gtgtggtttc accgtgttag ccaggatggt 54060ctctatctcc tgacctcatg atctgcccac cttggcctcc caaagtgctg ggattgctgg 54120catgagccac cacgcctggt ctatagctaa ataatgttaa gattaaaaca ttttttttaa 54180aaagtatttt tagtcgctta tataaaatgc attttaatat ttattcagaa agttctttcc 54240agagcctggt gtggtggctc acgcctgtaa tccgagcact ttggaaggct aaggtgggtg 54300gatcacctga ggtcaggggt tcgagatcag cctgaccaac atgttgaaac cctatctcta 54360ctaaaataca aaaattagcc gggtgtggtg gcggatgcct gtaacctcag ctacttggga 54420ggccgaggca ggagaatcgc

ttgaacacag gaggtggagg ctacaatgag ctgagatcac 54480accattgcac tctagcctgg gaaacaagag caccactcca tctcaacaaa caaacaaaca 54540aaattatttc cagaatgtta gcagagaaca ccacacatcc caaatcagta attacaattt 54600tctagttttt gatattctaa tattccaaca tactattttt attaccaaaa tgctggcatt 54660tttgttgctg caacaccatt tatctgaaat aaattaaagg ttttattaga attcttggct 54720ttctccaatc tcttgccact tcccttccct gcccctctac gaatctacca gtcagccaga 54780gaaaacaaaa aagaaacaca caaccacaga aaacgggacg tgaaactagc aggagcatct 54840ctcagttaag gaggaaagga atcagacccc ggttcatgac attccttttg gggaggctgg 54900caagagaaaa taagtctgct gtagcacaca gccctccaac atagcagaaa agctgccctg 54960cagtgggtgg taataaagcc tcctcaaact gcagtggggc tctgaagctt aactgaacca 55020gctacaccta aggaggcaga tcaaagagca ccaccgaaga ggagagacag accctgcagg 55080aagtggcaga tgagtagtgg tcacaatgac cagtcccgct atatagctgc tgctttggtc 55140tttcttttaa atgaaacctt taaaaatttt atacaatgaa aataattagg gaacaaaatt 55200tgtctcttcc tataagtgtt atgtgtgtta cattaacacg ttgaattaaa taagaatgca 55260tttatttaga aagctgaaga aaaaatgaag aggcttccag gtttccacaa aaaagtggtg 55320gctgtatcga tcacatccta ctaacatctc taaaaatcta tccagatcaa aaaggggaga 55380aataaaaaaa aaaaccccta tgacttaagt ctagagtaaa actaggagac agagcaataa 55440actccaaata ccatagaagt ggggaaatga gcagggtcca gcaggagcta cattgaagag 55500tggcttgtgc agaatcatac tgattaggga tcacctaagg ccagacggca cccgcctcca 55560ccacccaccc ctcaacagag cactactgag ggcaggttag agcactggca acggggtggt 55620taaaggaagg actacagaaa agtagttggg tccagcaatg gcaggctcag gaatgcacca 55680tgcatgaaag gaagacgggt accttggaaa gcagaagtgt cccttaagtg gctgtaaaga 55740ggtggaagca gctaatgaaa aagaatcttc attaagtgga tggagctgaa aaatcagcaa 55800gtggtgattc tgcccttact aaagcaacag aagagggacg ccttcagcca ggacccctaa 55860aagccataca aattcctctc tgctccactc taaggtctag taataacatg tccagaaaaa 55920aagtaacatc tattatagaa acataaacaa gaaaaacaga attcctaagt ccaaataaag 55980ttattatgga aagagtctgg caaatgagaa gcaaaacttt ccggtagaca aaagtacacc 56040agaaaaattc agtcataaac aacaggaaac tcgtaacacc acattccaac acaaagggag 56100aatgtagcag cagacaagac agaccaccaa tgatgagaaa tacaaattca gagagaaatg 56160agcacatctt aaatagaaat aagaatacta aaaaatactg aagaaatatg caaagtaatt 56220ttggaaataa aggctaattt tattataagg tgaccaaaga agaagcaata tgactaaaat 56280ttctactgag gacagagtct agaaaattaa aaaacaaaga aaatgaataa aataaaccta 56340actaaagaaa tgcgtatcaa agcatagtaa cgaaaaagat taatagctga ctacataaac 56400ataaaagctt tcacctggca aaaatgctat aagcaaggtt aaaagacaaa tggcaaactg 56460ggaaaaaata tttgcaaatt taccagcgtt aaaggactaa tctccttaac atataaagtt 56520tctaaaaatg aagaaaacaa ccgagcagca aaatgagcaa aagaccatat aaaactataa 56580acagctctta agtacaagaa aaaatgtcca tcatcattca taactaaggt aaactcaaaa 56640attgtaagta tagtaaaata ccatttctca attggcagat aagtaaacat ccacatttaa 56700caacacatta tgtttggaaa gttttaagaa aagagagact gttgtaaact ggtgggaatg 56760caaaatgtta cttttctgtg aaggagaatt tggcagtatc tagcaaaatt atatatgcat 56820ctaccatttg atgcaacaat cccacttcta acaatttatc ttaatgatac cttacatatg 56880taaggaataa tgcatgaaca taagaggatt cactgtgaca ttagtactaa cagcaaaagg 56940ttaaaaataa cctcgatgcc tataaatatt gataagctat gccgcatcca cacaaagtag 57000cagtattcag tcctacaaaa caaaatggaa aacacctctg caatgatgta ggatgattct 57060caggatacac tagaaagatt actttcctag ttctgtcctc tgataaggcc tacaagtagt 57120aacattcaaa accaaaggcc atactttgca tctaaaatct gacttctaaa tgccattctc 57180caacaattta ttggaaaaat aacttattcc aggactgaga ctgaatgttc gagattagct 57240agaaatttca aaatctaata gagtcatttt aaaagcacaa gaaggctaag gaatttgaat 57300atcatttaga gtaatgactg tgaaacatat taaatacaaa aacactgagt tcttaatatt 57360caaaaaggca gcaaaaccaa ccaaaaaaca aaattaaatt gtcactatta aagttattac 57420attaacttct tactctaaaa accttaaatc tattttatca tgccttttca gtagaactgt 57480ttttcaaggt aatcgaatgg catccaatga tgagaaaaaa cagtgccagg tatatatgta 57540ggacaagcag atggaatttt tttttcccta aacagccatt ttgcaatccc aatgagataa 57600cagattaatg atactgataa atacggaaat caggtgaagg gcagatggca cgttctggaa 57660agatgatgat agcagtgaca tagttattat ttatttattt atttttttgg ttttattccc 57720tcccaacctc ccctacaaaa agagcaaact gagaaaacca aagacccaga gacattatct 57780ataacaaaac catgaaccat tgcatataag tggacaaaac aaagctctga caagtacaag 57840actggttagt aaggaagcag atggaaggta actgactggg tttcttacag ccctgtgaac 57900aggaaaaacc tcaatactgc caacccactg gaaagcacag gccatctgaa aacagggcta 57960aaagtttaaa aaagttctac aggatctaat ttgcagatga ttacaaaggg atcatgatgc 58020agtaagctct gggcccttaa aattcccgaa taccaaatcc tcccaccaaa acaaaccatg 58080taccaaggaa aaacttgtgg gaataacttc caaatggacc aaatcaaaga taataaagac 58140caaaaaaagt tcaaatagtg tgtgtggggt agaggagtaa aggcggcagt ttcagaaagc 58200acaagggcat atttttgacc attttacaaa aacacagact tctccctgtc cctaaaaagc 58260aagaaaagtt atcctggcct atctctccct tgtgcaagta tgagacactc atttccctca 58320caaaaaaaga aaaataaata cataaccaac agaacagagt gcaacagtat aattattaga 58380gaaagagtat gtgcatatac atgagaatgg tgtccctaca gaaaatgaaa gtaaaccaaa 58440aaatatggaa ataaaacatg aaaactgtaa acaaatttca aactgagcta aaaagaattt 58500taaaaataac agatccttag aaatgggaaa tttcagaatg agggtaactg aagaaaagaa 58560agccatgaca caactaagga gtaattagaa atgcaaggaa aaatatcaca tcaaacacac 58620aaaaaaaact aaaactaaat tagaagaaac ataaaggtga ataaaacaat actactacta 58680gaaactgaag gcagaggaaa aatcttaaaa tcaaaaacaa gtaaagccat gcctgagact 58740ctcctagatg gtccagagac acagtgatgc tgtgcactgg caggaggaat tctgctgtaa 58800ctggtcctgg gagtgttcac ctttcctgta agtgttccac agcccaggga cacaatgtgg 58860tcaggagcac tgccaggaac accagcaagg gggagcctgc cacaataggc acaaggctca 58920cgaagcactc tctggcctac gaaaatctag tgggggtgct cttccctcac ccaaacacac 58980tctgtgcagc tcagcctgga gaagccccct caagcagcac tactggaacc cgtgcgagcc 59040ccagtggact gagataaaag caacaaacca aaatagcacc acaaaggctc tgaaaaatgg 59100tcactggaac cacagcccac aaatatatgc tagcacctac atgctaaacc taaacaggtt 59160actactgaag ttaaaatttt ttaaaagtac agagtctcct gtataataac taaaatgtcc 59220gagacacaat ccaaaactta tctgtcatac caagaaccag gaaaatcaca tgaaggaaag 59280aaaatcaacg ccaacactgg gatgagtaag atgttgaaat tatctgacaa ggatcttaat 59340gctgccatca cagaaatgat ttgacaagca attacaagtt ctactgtttc aaaaatgaaa 59400caatagaaaa tctcagcaaa gaaacagaag ttacaaggaa aaaaaaagca atggaaatta 59460tagacctaaa aaatacaata ggagaaaaat aatcattaga tgggctcaac agtagagtga 59520agatgacaaa ggacagaaat ctgaacaaaa gagagaaaat agactgaaaa atgaaccata 59580cctcaggtac atgcggaaca ataacaaatg actcaacatt tgtatcatta gaggcctaga 59640atggagacaa gagtgacgct gaaagaacac ctgaagaaat aatggttgaa aattttccat 59700gtttggcgaa agacacaaac ttacagcttc aagaagctga gcgaacccca aacaacataa 59760ataaaactat tcttcaggaa taaagggaaa ataaagtcat cttcagaaag agaaaaaaag 59820aatttctcac tagcagaact gcttttgaag actggctaat ggaagtaata aaagatggaa 59880tcttggaaaa ataatggaaa taacagaaac atgggtacat acaacagagc atcactccct 59940tcataagttc tataagtcat attggataac tgatacaaaa actgttaaca ggattaatcc 60000tcaaaacaat actatttaaa agtggggaaa gagacgtaac tgaaattaag gtttctacat 60060ctcattccaa gtgatggtat tgatggcagt gaatggtgat aagtcacatt atctataatg 60120tgatgcccac aatgaccatg aaaacaacta taaaaaacac ataaaaggcc aggcacggtg 60180gctcatgcct gtaattccag cactttggga ggctgaggcg gacagatcac aagttcaaga 60240gttcgagacc agcctggcca atatggtgaa ccctcatctc taataaaaat acaaaaatta 60300gccaggtatg gtggcatgtg cctgtagtcc cagctaccca ggaggctgag gcaggaagat 60360tgcttgaacc tgggaggccg atgttgtagt gagccaagat tgtgcgactg cactccactg 60420cactgcagcc tgggcaacag agcaagactc catctcaaaa caaaacaaaa caaacccaaa 60480caccaaataa atcaagggaa aatcttaaaa aatattcaag taatcgtgga acacaataaa 60540taaataaata accataaaca gaagacagaa acagaaaaca ataaaatggc agacttcgcc 60600ctaacatatc aataattacc ttaaatagaa atggtctaaa taaacaaaag gtagagacta 60660acagttttta ttaaaatgac aactatatgc tgtttacaag agattcattt caaattcagc 60720aacacaggat gaaagtaaaa tgatagtaaa agacagatca tgcaaacatt taaacagaaa 60780tggttacatt cctatcagat tcagagcaaa gaaaactgca aaacagaaaa atcttaaatg 60840ataaaggatc aatcaccaag acatagtaat tctaagtggg tatgaaccaa atgacagagc 60900ctcaaaataa atgaaacaaa accctatgac agagataaag agataaatct cagaagcaga 60960cctatataca tattctcaac tgatttttaa catatgtgaa aaaaacaatt caatgaagga 61020agaacatacc tagaaaatac acaaaaaaac cccaaatgga ctcttggtat ttaatcccag 61080agaaacacaa actttttgtt tataactaac atcacagtta atggtaaata ttatagtctc 61140tctcaataaa atgaggaata aagtaagaat accttctacc gaaggttcta gccaatgcaa 61200cagaggaaca gtaataaatc acataaagat tggaaagaaa gaaacctgtc ttgatttgta 61260gatgacatag ctggttacca gaaaacccta tgaaatctac taaataacta ctagaataca 61320tcaatttaga aaggttatag gatacaaggt caatatataa aaatcaattt tatttcaata 61380tttcaatata gtagtaaaaa acaagtgaaa agcagtattt aaaaatacca ctaatagcat 61440tagaaacata ttttaaatgt attcctaacc aaagagtaaa tgatttcaac attaaaaact 61500ttataaaact gctgagataa actaacaaac atttaaataa atggagagag gtacggtgtt 61560caaggattgg aagaatatat tgttaaaatg tgaattctct ccaaattgac ctaccccgat 61620caaagcaacc caaatcaaaa tctctgcagg ctttgccctt tttttcattt gtaaaaatta 61680actgattcta atatttatat gcaaataaaa gagtcaaagc aacctaatga aaaagttgtg 61740ggactcacag catctgattt gaagctgcag taactataac agtgtcgttt tggcaaaagg 61800accaaacaaa ttaatagaac agaatggaga ttccagaaac aaggctcaca cacagattga 61860ttttcaacaa aggtgcaaaa gcaattcaat ggggaaagag aagtctcttt aacacatggt 61920actggaacaa ataaataaaa ctatttagaa aatgaatgac atccactatc taacagcatc 61980acaaaaattt actggtggtg gatcacagat ttacacataa aatttaaacc tatattatag 62040tattagaaga aaaaataaga tatttgtgcc tttgggctag gcaaagatat aaaaggacaa 62100aaaaaataaa agaaaaaatc aattagactt caaaaattaa taatttctgc tcaccagaat 62160acacttaaga aaatgaagcc acagacttgg agaaaataat cataaactac atatcagaaa 62220tgggcctttt atatagaata tacggtattc cccacttacc catggggaat gctttccaag 62280acctccagta gatgtctgaa attgcaaaaa ccctgtatat accattcatg atttattttt 62340ccttcttcac aattccataa atactcattt ttaccacaga ttttagcaac ctcaacatat 62400gatttttaaa ttctttcctt aaaggaagca ctttccccag cttctctttg gcatattcga 62460atttccaaca tcactgctct tgtgcttcag ggccattatt aagtaaaatg agggttactt 62520gaacacaagc atgcgatact gcaacagtcg atctgaccaa tagactcaaa gtggtgggta 62580gtctagacaa catggatata ctggaaaaag gataattcat gccctgggtg gaaaggtggg 62640agatttcacc atgttactca gaatagcatg tattttaaaa cttaagagtt gttggctggg 62700cgcagtggct cacacctgta atcccagcac tttggaaggc tgaggtggaa ggattgctag 62760agtccagggg tttgaaacca gcctgggcaa cacagtgaga ccctgtttct acaaaaacaa 62820acaaaacaaa acaaaacaaa aaaactaaaa attagctgca caactgtggt accagctact 62880cagaagactg aggtaagaag actgcatgag cccacgagtt tcagtttata ataacctgta 62940gtcatgctac tgcactccag cctggatgat aaagactttg tctcttaaaa aaaaaaaacc 63000aaccaaccaa ccaaccaacc aaccaaccaa ccaaccctcc ctccccaacc cacacccacc 63060aacacacaca cacatcaaaa actcttgagt tattcatggc attttctact taatattttt 63120ggaccactgc tgatcaaaga taactgaaac caaaaaaaaa gcaaactaaa gataaggcat 63180aatgctatat tttaagaaaa tatctatagt caatagaaat acaaaaatcc aatattaaaa 63240atgaggaaaa gacactttta gttaaatatg tacctactat atgctttgga aactccactc 63300ctacgtgttt gcccaggaga aataaaacat ttccacaata agactagaca gctttattca 63360caatagttcc aaactggaaa caatccaact gtctaccaac aggtaaaaaa acacattttg 63420catattcata taatgcaaca gtactccata atttttttaa aggcactata aatcaatgca 63480ataacatgga tgaatctaaa gagtctcgat gcagtacaag tatataagat tccattcata 63540agaaattcta gagcagacaa aagtaatgta cagtgacagc aattaggaca gttattgtct 63600gttctaatgg gggacagaga ttcactggaa gggaaaacat gggaactctg tgttaatgga 63660catagtctat accttgactg aaatatggtt acataaacgt aatttttaaa aacaaaccca 63720gttaagccct gggtatttca ctgtattaaa attttacccc acacaacatc gacaccacca 63780ttattaccac cacattgatt aacatttaga gagcagttag ggaatcacat tattttgaaa 63840attagtaaat aatgggaaag aattaagcat tattgtatct gataagtaca tatttatata 63900catgtataca ggtcctatat gtggatctgt aatttagggt agtcatagag ttgatgagaa 63960agagtttttc ttaagaagta ttacaggtaa taaaggaacg aggaataaag aattcaaata 64020tcacaatttt gcaaccctta atgaattagc agatctaggc aatcagaatc tatgataaaa 64080attactagaa aagacagaaa gcaagtgaga ctttaagtta ttcctgtaaa ccaacatcta 64140tgaagtattt gggaaggaaa taaagtctga atctgttttt cgtttttttt gagacagagt 64200ctcactttgt tgcccaggct ggagtgcagt ggtgtgatct aggctcactg caacctccgc 64260ctcctaggtt caagagactc tcctgcctca gcctcccgag gagctgggat tacaggtggc 64320tgccaccaca cccagctaat ttttgtatat ttagtagaga cggagtttca ccatgttggc 64380caggctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 64440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 64500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 64560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgctaggcac ggtggctcac 64620gcctgtaatc ccagcactgt aggaggccaa ggcgggcgga tcacctgagg tcaggagttt 64680gagaccagcc tggccaacat ggcaaaaccg tatctctact aaaaatacaa acattagcca 64740ggcgtagtgg tgtgcatctg taaccctagc tactcaggag actgaggcag gagaatcacc 64800tgaacccggg aagcggaggt tgcaatgagc tgagaccacg ccaaactgta ctccagcctg 64860aatgacagag caagactgtt tcaaaaaaaa aaaaaaaaat taacaaaatc ttatttctag 64920tggtttacta ttaattatgc tggcaatttt tagctgaaat atattaagca gccttgctgt 64980tatttttatg aattctgcaa caataatacc aaccaaaata tctatacagg catatcccag 65040agatactgcc agttcagttt tagactatgg caaataaagt gattatggca gtaaagtgag 65100tcacacacat ttttttgttt cccagtgctt acagaagtta tgtttacact atactgtagt 65160caagtgtgta atagcattgc ctaaaaataa gtacattatc ttaatttaaa atcattgcta 65220aaaatgctaa tgaagtgagc acattttctt ggaaaaatgg tgctggcaga cctgcttgat 65280gcagggttgc caccaaaatc acaaagtatc tgcaaagtgc aataaagtga gatatacctg 65340tattgtcctt tttctcctgt tattacaagc actattttaa gtcttcaact acagaatact 65400tgtagactat ttaatgaatt aatattttca tcatatttat attgagaaaa ttaagctgaa 65460tttagttaga aaaggtttag aatatcatca actctgatga tgagttggaa gctactttat 65520ctgtcatttt tcacataaca ctagcttgcc cttcaaatca tatatttaaa aatgacctta 65580agatacacac caagtaggga ttatgttgtt ataaaaagct agaaataaaa ttaagacttt 65640aatttcaaag ataatttctc tataaagaat agcacaagat atgttcaact attctcagag 65700atgcatcaag atatgacttc ataactatta gaaattatat ctatgatcaa tcatattaca 65760gagatattgc tgttatactc cttgtgccct ttaatgaatc acctttgaaa ctgaataggt 65820agcagcatag aggttgctaa gagtaaatac atttaccact gctgccagaa atgaccaaag 65880gtgcaccaaa aagaagaaat actggtgaat tcccacaaac tacaatgaag aaccttataa 65940aataacagaa caaatgaata taacgtaaca tacagccatc tcttggtata cgtgacaggg 66000attggttccc ggactgcttg tgtataccaa aatccatgca cactcaagtt ccgcagttgg 66060ccctgcggac ctggtgtgta tgaaagtcag ccctctgtat atgcagcttc tgagtcccac 66120caatactgga tcggccctta gttgagaaaa aaaatctgca tgttagagga cccatgaagt 66180tcacagctgc attgttcaag ggtcaactgt aatataaaaa taacatttac tgctagagtt 66240ggtactcatt ctagtgttta tatacttgag tcttagagca atagccttgg gagacaactt 66300ccaggaaggt acctgtttgc ataccttctc actgcaatcc atcagtggct gccaaaaggg 66360tatttttgtc tcttgaacca gtctcagtac gggatgggga agcctatgtc cttcagaatt 66420ggatggcata gtacacagaa gtagacagta cagtagcaag ggcatttgct taaaagcaga 66480tagaattggg agcctctaca tctttctgtg agaattcagg gctcagtttc ttaaggtgta 66540aaatggaaat actactatgt atctggacat attatcttaa gaattaaata aaacactgta 66600ccatttcact caaaaggtgc ttgatcctgg gtagccatgt agttcacaaa tgcagagttc 66660aacagaaagg aaaaatatgc agaagtggta gccttatttg aattatcttc taaccagcag 66720taagcatact cttagccagt gagaaatact agataatcca taccccaaat gcaggagaac 66780acatgagact tacatataac aacacttcat tcatgaaagc aatcacaaga ttagctctga 66840aaaacggtga ttatattaca aacacaaatc taagtatata gataacaatt tgtgagtttg 66900cttatttggg aattagtgcc tagagataaa ggtcagaagc aaaatcacac acccctttgg 66960tgaatcaaat cttcctttga agaattaagt acctcaccta atcagaacag agtccctggt 67020atagccaccg tcatattctg tagtttcttc taatgcttgg aaatctagat tctgcaaatt 67080taagaaaata tgtcttagta tctgctatac tactgtatca ttacaaacaa taaattgata 67140catgactttc tgcagacacc tgctttctta cccggcttcc acatataagc aattcaattt 67200cttctggtct gaataagtac tttaagggag attcattggt caccatatga aaacctctcc 67260gaaaagcctt gaactgtttt tctactgatt tattgagaat gtagtcagaa taaagattga 67320caaattcctg tagaaaacat taatcacaag aacttcttat aatatgctat gcaaacaaaa 67380cacaagttat tgggctgcat agctttttaa tttttccaag aaaaaaacat tcaagaatat 67440tcttaaaaat tcaattttac acctacaaag aaaactactt cttacaattt cacaattctc 67500tgttatggcc aatattaaac tatgaaaagg ccatatacat acaactgtca tacatataaa 67560tgtcaatttt gacaatgtat taaacagaac tgaatataaa tgctggagac acagaaaatt 67620ctttaggttc gctcaactag gtatgcagac agacacattt aaatcatgta cccatctatt 67680tataaatgcc accgttttaa gagagtataa tgtatttgat ttaatcaaac caggaagtcc 67740catgttttct accatagagt atttggtaag ctgcctacgg tatccagttc agcaaaaatg 67800tggaactact gactggcaca taaactactg tatcaaatca taaaaggaaa atgtgctttg 67860tacccttagt gtgtataata cttaataaaa aaattatacc aatataaata tgtttctcaa 67920aggtgtacta tatgttcgtc ataatgtttt ccttgtcatg ttctgggtta ttcccattcc 67980agacgaacac attttagtca acagaagccc aaatctaatg taaagaatac ttttcagaaa 68040agtatttaca tcacacacag gatatgcatg tgtaaattaa atgtaatttt tatttgttct 68100gtgataaaga ccttaatcat gaggttcctg tttacagctt aatacaggaa acaccctgct 68160tcttgcttta tcctacaacc acacttagct attgactgtc tcctgtttca tgtcctcttt 68220ctctaaaagg ggctttagtg cccaactgtg tgctttcata ccagcttata ttcaagattt 68280agaaaactgg actctagcat ttacataaac ttggttactt gatttgtgtg tttttttttg 68340tacagactgt attagatact tctttaaaaa ttataagcat acatgctttg aaattattag 68400agacaaaaca tgacataaaa acatttatta ccttcctgtt ttcatttgta attggaattt 68460tatcaccatt ttcctttaga tcatacatca ttgggttacc aaaaagatct gtctgtgata 68520tctggaaagt gatcatcatg tcgtcttcca cattcccttc atactccaat aaatctttta 68580aactctgata cagaacctag caaccagaaa tggtaaattg aggccaccat agaactacaa 68640aatacaacaa ataatttata tcactcttct tagaagataa caaaaatctt atcaataaaa 68700attgtaaaaa gtgtagaaag tatccttcca gtcccccaaa tagtttccaa cctataaaag 68760aaagactgtc agtcactgtg atgttccatt ctctgctgag aaactccaca ccaccaattt 68820agcaacagat ttcacactgt gctctataag aaattctgtg gtgtctcaag gtgagggtgg 68880gggtggcagc aggcacactc actgtagcta ccaagaccct tagccagggc tgctctgatt 68940ttacctatct cttaagattt cactaaagca caaacaaaat gtttagaaac acttctctat 69000ggaatcaatc tttaaaaagc tttgtgctga cagcaatata taaaaatgtc cccctttgag 69060tttttaagtg tttgtcgtac ttcggtcaga ttaaaacatt atattattga taaaagtatc 69120aacaaagatt ctaaacaaaa actttaaaat tttgatataa ataaattgta tttttgcatt 69180taaataaaat ctaagagact gaattaaaaa atgacaactt actgggtgag agtctcccaa 69240gtcacgaaaa gttccttttt tccccattag ctttctgtag acaaccatgg gaaaatgtac 69300atccagtata cagttattgt aaatagccag acccagtact atgccaatca gagtaaactg 69360accctcagtt tcaaaagaag acggattaaa ccaaaacaat tttgtagatt catcgtatgt 69420gaacatacct gtaagaaatg attttttaaa atacattact tttgtatttt attaaggaat 69480gataacacaa atgtaaattt

aaaataatta tgcatataaa acatttaaaa tatatgtaat 69540taggcatgca acattgattt ttattatatt agcacttgag aaaacaatgt ccacatatat 69600ctaactgtta taaattgggg tgaatagtga tatgtaattt tatgaagcca aaggaataaa 69660gtagtaagta atttggtggc atcgggtaga aaaatacgtt cattaaattc aagctttcta 69720attttctatt gtaacataag aaaagttata ttcatacttt atccacccat agctgaaaaa 69780taaacataaa aaaaccaaaa aatggctata ttttgaaagt gtttcattta taaaaatata 69840attacttata tttgtactac acgtactatt tttgaaaaca attttttatt ttttttcttg 69900agatggaatc ttgctgtgtc gcccaggctg gactggagtg gcacgatttc agcttactgc 69960aacctccgcc tcctaggttc aagagattct cctgcctcag cctcctgagt agctgagact 70020ataggcgtgc atcaccatgc ccagctaatt ttttattttt agtagagaag gggtttcacc 70080atgtttgcca ggatggtctc catctcttga cctcgtgatc tgcccgcctc agcctcccaa 70140agtgctggga ttacaggcgt gagccaccac gcctggccta aaaaacaatt tttaatcaca 70200gtaaggaaag gtttgttttt tcaaaatcag acaatggtgc catgaaattt ttctagcatt 70260ttcctaataa aatcaaatga tctaaaatac ataaacttgg ataaatctga tgaacttaga 70320taaacaaagc tatttaatac acattaatta tgggctaata tgtaaaaaga atagtagttt 70380agtagtcttt caatttcaca tggtcttcac tatgcaatcc tcaatcacgg aggctttttt 70440tttttttttt tttttttttt tgagacagag tctcactctg tcacccaggt tggagtgcag 70500tggtgcgaac tcggcttact gcagctccac ctcccgggtt catgccattc tccggcctca 70560gcctccggaa tagttgggac tacaggcgtc tgcgtagagg tcttttttat atcactcatt 70620ggaaaattgg ggccgggtgt ggtagttcac acctgtaatc ccagcacttt ggggatactg 70680aggcaggcag atcacttgag ctcaggagtt caagaccagc ctgggcaaga tggcgaaacc 70740ccatctctac aaaaacacaa aaataagctg ggtgtggtag catgcgcccg tagtctcagc 70800tacttgagag gttgatgcat gaggatggtt tgagtcttca gcctgggagg tggaggttgc 70860agtgagccaa gatagagcca ctacactcca gcctgggcat cagagccaga tcctgcctcc 70920ctccaccaaa aaataagact taaaaaaagg aaaattggaa tctttggttt tatctgaatg 70980agtactacta aaaattgatt tcttcccttt taaatgacta gggaaaagaa attttaattg 71040aatttagaat tttttcgttt tactaaatgt ttagtttcta ctgtgaaaca atgaaaacat 71100cttaagaatt aagtctgaaa aaaaatctgt aatttccaat agaaaagtgg agcttagaaa 71160tcagaaatca aattatatag tatataagta ttttaacgag agtttttaag aattttaaaa 71220tctttgaaaa tgttattatc actttaagta taaaaatatg gagctttaac atgtttcctc 71280gatcactaga tgcatgaatt aagcctaggc cattttctga atgcaaaatg gtttgaaaat 71340taaacaactc atatttgagt ttcatggttt ctatctaaac attttttaca ggccaagtta 71400caatatccat ggaaatgctt tcagttataa atatggtatt aacaggatca agaacagctg 71460acaagtaatt acaattaata aatttgttca agttggcaat ggtgtgtcta aaaaattaat 71520gaatttgata aaatgtcaca aatgcccttc cttggaacca agagttataa gctataagtt 71580aatcagaata taactctgaa gttccatttt aggcacattt tctcatgttc cagggagaaa 71640agcaccataa cctcctgtaa aatattcctt tttgatactt ctactgccat cacttcagtt 71700accttactgg aactgcttaa tctatgtttt ttcttacaag tatttctgca tataatgcag 71760tatgctttat agctaaggtt taacatttct agatacatca aaagctagag ctaaggttta 71820ccaaatttaa gaaccttatg aagtttataa ttttgtatct caaaaaaagg gcacggtctt 71880atgtatctgt gatttactta gaacaaacta catacaaatg gcatagtttt ttagttatta 71940ataaaacact agctagacta tagataaagg gggtgtgtgt gtgtgactaa gaacagtaat 72000agaaaggtca actcaattag aatcaacagg ctataacagc tcaaatctca aagctgaagg 72060gaaatgacat acattattct acgcatactc ctcacaattc tgatggtacc ttcagtggaa 72120accttttcct ataattgtca tgtactcaat tttaaatgta agaaagttca gttactaagt 72180ggttgtattt acagtagaaa ctaaaccagt agagaattat gcagttaagt agactaccca 72240aaagactgca tcttttccct gttctacttt atcacaaaag ggcaaaaact aaaattctga 72300tctttcactt tcaaatgtcc atatcccatc agcctttccc aaactgtgtt caacaacaac 72360tcttctggta gggcagaaaa agagttctgg agttcaaaaa gtttggggga aacattacct 72420atattaagcc ccaataaatc ccatagcaaa taaaccttca tgtgatatca gctagtgttt 72480cttaactttg agcatgacat acttgtgaga cagtctctca acactcttca aatcattgtt 72540ctttgaagtt cacaggtcag gaaaaccgtg agtttgatga tggttttaga catcaaaaag 72600caaagaaatc cctccaactg ttccaacgta ccattctaaa gtgctttcga ttcaatttac 72660caaagagata atttccttca ttagaatatt ccttctaaga aatagaataa tttgaataaa 72720ttagtgatac aaatcagtcc ctaaaaccta caaattcagt catgatgtgt gattccgggt 72780aatccatacc aaatccttct tttgctgctc ttcatagctg agaatattta tcaactaaga 72840taaacaaata actaactcaa aaagacaata tgacaccata atcacattac taaactattt 72900accaatatct ggattgaaga tttcctccac aaccagctga aaaaattctt tggaaacacc 72960tccttcatca actccttgtt ctccttcaaa ttccacatac aactgcttct tcaagtctgc 73020aggattttcc atagcgatca tctctagcta gtgattgaaa agacaaacat gaaaagaaga 73080tgactgctag tatcaagaca aaggcaaaat aaaaacaagg agtagggtta ggaaacaaat 73140acaaattttt gactttttgt atgtaaatgg aaacagaaaa caaactgata aaaagccaat 73200gaagacaaag attcagtctt aatgataatg aaaaagacac aaaagatacc ggcatagtaa 73260aaagcttacg aactttactg atttattaca aaattaacat acagttaatt aagattttat 73320tacttgtgtg ttaacaagtc tggactacac agttaggaca tggaaaagca gctaaatact 73380ttcagcaaaa acaatggagt agaagtctga cctcaaaatt gtccattagt agtcctttat 73440ttttattaga atagaaactc aagagttaag tcttaatatc tactgtcata agtaaaatat 73500ttcaattaag aaaagtacac tgaatagtaa agcagtttat gatctctcac aataggactt 73560agtacaaatt accacctttt aatgggttag aacccatctt atattcacat tttcttaaaa 73620gtaggtgaga cataacctta ctcatgtcct tacttttttt tttttttggg atgaagtctc 73680actctgttgc ccaggctgga gtgcagtggc gcggtatcag ctcactgcaa cctctgcctc 73740ctgggttcaa gtgattctcc tgcctcagcc tcctgagtag ctgggattac aggcaggtgc 73800cactacacct ggctacattt tttattttta gtagagacgg ggtttcacca tgttggtcag 73860gctggtctcg aactcctgac ctcatgatcc tcccaccttg gcctcccaaa gtgctgggat 73920tacaggcgtg agccaccgca cccggccctt actcttaaat tgtaagttat caaactttag 73980tgtctagcat tttctcaact ataaatcttt taccaaatat tcttgtgtac ctatagaatt 74040atgaatatgc agtaattaac ctcattgaga ttaatcacaa aatcaacact agcaatatta 74100actgtttata ctgcatttta ttttatgtat ctaaaattta ctaacagtac gactgtatcc 74160agtacatgga cagatactgt atccagtatc catcatatct cctaacttgc cttcaaacat 74220ctgttggctg gttaaaaaat tttaatcttg gagatgaaaa tttaccaaaa caggatatta 74280aatgtgttca ggttattcca gctattccaa acagagtttt aaaaatggtt ttgaaaaatg 74340caataatcat tgaaataact gtaatacagc acaatagtag ttaaggtaat aatactcatt 74400tggacaatct agtctgtcta taaaaaataa aatctcataa attaacgcat caggtcaggt 74460tgccctagag cattgttttt ttcaaacttg tcacctgcaa ctcattagtg ggtagtctaa 74520tccatttagt ggtttgcaat cagcaatggg aaaatcagaa tgcattccac aaagtaatgg 74580acacctactg ttttgtgaaa cttctgtttc aattagatat cagacggata tgtatgtact 74640gggatgctat gtaaaatact agggattgca gtcaaagagt ttgacaaaac tagctgaaat 74700tatcttctgg gtatcttcca actttagggt cccattctgt gaatttagtc tttagattta 74760aataatgtct gttcttgttc aacacccatc tctaatattt acaaaatcat ttttaggcca 74820cacagtttga gactgttttg ttgtggaaag atcaagagtc agtcagaata caccttacta 74880gtcccacctc tcacttacca taacttttaa cctttaaact ttagcaagcg attcaattaa 74940gcctcagttt cctcaacata ctataagttg tgaaggctta ttaagaatat atgagaaaga 75000gtgttacctt ttaaaacaat tttgctcttt gaacaaaaga tttgcaattt tgtgtcccca 75060aaggaacttg atatagacta gttccaagat ctagagtcaa acaaatgtag atgtgaaaat 75120aaccttggag aaaaaaaaat agttccagtt ggaaacctgt ttcaagctac ggagtatata 75180gaattctgag gagactaaaa ttagaattaa catcttagct caaatgtggg accaggccaa 75240cattccatat tgccatgtaa tccattctga gtttccttat gccagttccc aactggctag 75300tgttaaagat gtaagtcagt gtttatatgt acctttacat ccaaaggtat acttgctttt 75360atcaaatata atgtattcca aagtggaaaa aaaaaaaaag attatctggt atttagacta 75420tgcattctac agcttttccc cactaacatt catacatgag agctgactcc tcatcatcaa 75480tgtaaaacct tttaaaggac gtggtggaga atttctacag gattgagaag ccctgtgttc 75540tacctagtct cagctctacc taagacatgt caaatgacaa ctgaagcaaa tttatcttta 75600ttatccataa gatgttacat gcctgtccac ttccactgat ctctattcta tgctctccac 75660cttggtgcca aaagaatgta atgcaatgca atatgttcta ttgtaaatgt aaacatacaa 75720ctttacctat ttcatctgta aaataagcat aatactatag caataaatta atgtttgaac 75780tgcttagatt ccctgcataa agttagtgtt taaaagtact agctgttgtt gctgttattt 75840tattggttct aaaactctct tcaatgatga ccaataattt tttttttagg attatgagtg 75900atttttattt tccttattct tttccatatt tttccatttt ttacaacaaa tacatttttc 75960agagtcagaa tatgttcgaa tataaatatg taaagagagt aatttgaaaa gtagttggta 76020actgaattct gtagacaaaa attttgttat tacctatttt agattaccat tctgctacac 76080ataagtgatt tcataaaata ctgtgccact taaaaataaa ggccgggtgc cgtggctcac 76140acctgtaatc ccaccacttt gggagaccaa ggcaagcaga ttgcttgagc ccagcagttt 76200gagaccagcc tgggcaacat gacgaaacct tgtttctaca aaaaactcaa aaaaaaaaaa 76260aaaaaaaaat tagtggggag tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 76740nnnnnnnctg taagtcagaa gttgttttaa atgcattaga atatttgaaa tttggaagac 76800tttgtcaaaa cattctaaaa aggagaatgt gcatgcatgc ctgaatttat ttggtataca 76860gatgtgaatt tttaggtatt tttaaaagta cagcgtatgt tgtatttatt taatatggac 76920actagcaaga gacagagatt actggggaaa aataaacaaa aacatcctgg atataatcat 76980agctacctct tcactgcgta acttcctcct ctttaggact cttgacttca tcaattgctg 77040gataaaatga tttttaattt ctgaactttt taattggcca tgatgaagaa atcctagggt 77100gttcattttt ttaaaaaatc tgacctgata tcaaagtatt tgcagaatac ctagcaggat 77160gacatcttaa tatcaaaata tggtattact agacttacag gaatcaaaag gaaaacctcc 77220tcacttaaaa tcttagtttc ctgagctatt acatcaaacc cattcataat ttaaaaaaaa 77280aaaaatcttt aattgcatga atatttaagt agaagcagta ctcagataag agagacctct 77340aacacttaag aatgcctggg atttctaaga aaggctagct tcaactactt cagtcaactt 77400gaggcttaaa ctattgcctg agatttctag gttatccaga ttaatttttt catgttcact 77460ataaacacat gaagaaaagt ctgttacatc tcaaatgtag tttaaatgtt tgggtaatac 77520caaatcacat aatcacgtat gtccatgaga cctggaatcc tgacttctac ttccaattct 77580gccattaatt tatgtaggct agaatctctt ttagatttca ttttcctcac atgtaatact 77640aagaagtttg atgtttcatt gtcttaaatt attatataat gctgaggatc tatgtatata 77700taggcttcat tctctcattc agtttctgag aaattacttt gagggcataa actaagaata 77760tttcttggtt tcatttttta tttcaattag aataaatcac aagaacaatg gaaattacat 77820gttaaccaca atatactgta tgtgtaagat aaaattaaat atacctagag cagcacagaa 77880taatcttatt tttaaagctc tgtgagtcaa tgaatgaata aaagaattaa gttcgtgttt 77940caataataag agtgttcacc aacgctggaa gctgggagct gaaaatgctg gaagctgggt 78000tagactgggc tattagaaac cagatgaaaa gggccagtga ctttcagagg ggattttgca 78060gaacctcctg gtagtggggc tttttatttg tgccaatcac ctttatttgt gctaattcct 78120atttttgaac ttccctattc aattccacat ctcagccatt ccttttggtt tttaggtatt 78180tatgtgaaat tctagaactc cctgactaat tggaaccaag tttcacacca ccatcatcct 78240ctattccaag cttgctgccc ccatgatcct cttaaaaatc tgagtttccg aaagacacat 78300acttgtatat caaaatggcc ttcctttctc tatgtatgct cacatacata catacataaa 78360catacatgca caaacacccc aacccaagat gtaccaaaag ccaccttccc aaatattgga 78420gtctggcttt atcaaaatat ctgtggagta ttaccagaaa tacagattca taggctctac 78480aatacagctt ctaatctaaa cctcttaaag gatagggccc tggatttttt tttttttaga 78540acaattttca cgttcagatg tgaaggcagt ttgggagacc tcatgatggc aatactatgt 78600tcttccataa acttataacc tctatcttct cctttaagaa gtaatttaaa aaatcttaat 78660acatactatc cttggaagtg cttcatctaa gaaaaatgag aaattatcta cttgtaaaag 78720aacacgcaaa tgggtaaata tgtaaaaacc tcatcacatt taggagtgtc ttcattcaat 78780gaaaggtgaa gtctaagctc atgtttatca ctgtactgtt ttctaaggaa caataacaaa 78840aagtactgaa agtcagtcct aggggaacta tggcagtaag ctaaacaggg gtagggtggg 78900ggtgttctta ggcagttcta aagtgtttgt gaagagacaa tttatattct attgattcta 78960aaattcactt ttcttttttc acattatctg tgaaatcaga ttataccata caaatgacag 79020catcttacaa tgaattgctg gtatttttct gtcttagcag taataaaatt atagtgtttt 79080ctacaatcag agattaaaat actacaaaca tgaattcttc tcttgtcatc aaagacaatt 79140tctctaaaaa cctcaggaaa aaagctgcta gtctgctttg aatatcacaa tatacaaaag 79200caatgaatat atttttcttt tttaaaaaaa tccataatta ttaattcaag ttaccactcc 79260tcaacagaaa acatacaaga gtctcatata tgaagtcact attttctggg cttgatttct 79320aaactaagga gtgaactctt ctttcagctc ttaaagccga agatcccata attttattta 79380tcaatattaa gcttaccaca aagtttctca tttcttcttt tctacccaac gctgctatac 79440agttccaaaa ttagcaagtc ataaaaaagt ggccaatagt ttgttcactg tgatatataa 79500gaatgaggct taaaaagcag agattcttgg gaaataaggt cttgaaaaac tcatcactac 79560ttttgagctt tcacatgaga gaataatctt tttttttttt tttttttttt tttgagacgg 79620agtctcgctc tgtcgcccag gctggagtgc agtggcgcga tctcggctca ctgcaagccc 79680cgcctcccgg gttcacgcca ttctcctgcc tcagcctccc gagtagctgg gactacaggc 79740gcccacaacc gcgcccggct aattttttgt atttttagta gagacggggt ttcaccgtgg 79800tcttgatctc ctgaccttgt gatccgcccg cctcggcctc ccaaagtgct gggattacag 79860gcgtgagcca ccgcgcccgg cgagagaata atcttaaacc actttctact atagaccttc 79920tactatgtgt tactaaaata tgtggcttta tcataatcat cacctgctgt gaatacttag 79980ttcaaaaaat tacgcaggtt atcaatttta cttctaagac ctattttgat gctggttttt 80040ccagtgtttt cacattctgt tatttaaaaa aaaaaaaaaa aaaaaaaaga aagaaaagga 80100agaagagaaa gcaagggaga aagttagtta agagtaaaat ctgcttagtg ggagcattta 80160gagtccctaa gaagcttctc agtgagcatc atctctttct actttaacag tttattaaac 80220tgcctaacac acatgacttc tgaagtgaac tgcttttgtg ggcaggcaat attcaagtac 80280atatactatc tagtgaatcc tgatttataa taggccacac catattatct agttttgata 80340taattaagca gaacttgcta aaaaaaaaaa aaaaaaaagt taccataatt aaagagctca 80400cagttaattt tatactaaca cctgagaagg ctttaaaagc ggttgtttcc ttaaaattct 80460agaatagaat ttaagaaata gaataaagta tacaccaagt acttaacttt tgttgtttgt 80520taggcttgct ttactagaga aaaaaacggg gggactgagg aaaataagac tgagtagact 80580tctgagcaga agacagataa ggaaggtaga caattttttg aaccctggaa tgaatcttac 80640agtgtaattt cattgctttt aaaggctaga catagctttt gatgaagaca gcagaaggga 80700tctcacctca agaagccaaa cataaagtat gtagcttcgg catttgcagt tctgctccaa 80760caatggatag tcatagacag acctcaacat acctttcata aaacatcact cacattctta 80820taaaataccc tatcattctg ctaaactatg taaagcaagg tgctggctgc cttatgatca 80880gtcgttaagt gtattaatta ttcatgtgtc tcaaaactat gtaagtgaaa taaaaagggt 80940accagaaagc aagcaataat aatgacttca ggcagttaaa gccccctttt gtctctgcta 81000gaaaaccacc cgagttgctt aaataatgca gaatctttct ccaccactat aaacatatct 81060gacattaaaa acaaaaacaa aaacagtaac aaagaaaaaa acacacaaaa tctctgcaat 81120ttgtaatttc tgagatgtca actttttcca ctgggctata gttttatgaa ttctcaaatt 81180tagacaattc taaaactaaa atttagacat tctaaaacta aaatacattt taaagtaaaa 81240atcacatttc ctagttgtgt cagacaactt tctctactag tatactatag tattattaac 81300taccttaatt ttggttgaca ttaaatttta cttgaagatg ataaaaaagc agcaatgaca 81360gggataccta atactagaaa tacaataaaa ctctgagaca atacacctgt gacaaatgta 81420aattctggta aaatgtgact gacaattggt ttctgtcttc tggccactct tatattctta 81480aatagataca ctaaaaataa tcttttgtgg agaaaagcaa agcaacaaat ggcaacccca 81540tctggaaaac caggaagtta agacgagtac ctgagtttcc agtatttccc tgctcgatct 81600ccctgcactt gatgttacag gcaccaccac gaatccccaa atttttggaa tagctgctgt 81660tttccagaag ttccaagacc cagagttgtc tctatgaatt tttaagtggg aagaaactac 81720cacagccagg cagtgccgtc catacaggga tattctaagt ggcccagcag gactcaactc 81780ttactggcaa caggcgctgg tctgccatgg tcacttcatc aagttcacaa taaaacaact 81840ctgaatttta catgatggaa ttttttggac actacctata attttatggt atagttttac 81900tgtaccattt ctcccagttc aagacttcac atccacactt attactgggt atctccacat 81960ttctcttgtt ctggtctttg cccaacactc tattctcttc tgaatgaaag aaactttgga 82020tctagtattc aagatttaga aaacccactc tttttcaaag atagagtgag cattatataa 82080tacaaccgat actttgtaga aaacatctat aaacttggac aggaacaaca aagtataata 82140cttatatcta agatcagaaa tgtccatgtg ttcctatgct atatggtatt tttttcagtc 82200acttttaaaa tgaattcact gaactgtatc atgatatccc cattactagg tttttaatct 82260agcagcccaa cttacccgga caagtgcatc atctatgata tggtcacgtc taactttgag 82320tctcaaatat ggattcaact gctgtccttg aactaagctg tagagaacag tgattcttcg 82380ttcactgtac atgcgaattc tattgtcata atataatccc aaattctttg tgacagcatt 82440taatataaag ggacatgtca taaaagagaa tttgttctct gtttctactt tgaaaaaagt 82500ataatcttta tccatttcta gaacctcatt cagtggttca ttaataaact cttcaaaagg 82560gataagtggt tttcgacaat ccagggtttt aacaccaagt tcagtttcca gggggtccac 82620tcgaggacct ttcttgtttc ttctttcttc tcccaaaagc tcctgaagtg tcagctcact 82680ggattcaggg atgggctctt catcatcttc ttcattgtga tttgtgtcca cttcccctcc 82740cactacattt gcatagtaaa ccattttcaa gcacttcgaa gcagcaacaa tggcatcatc 82800atcgttcact agatttcgac tgttaaattc attgcttatg actttgtaag taataagttg 82860ctgaaatgtc tccatcattc tccgaatctg gtctgcattg tatttagacc acagtctgat 82920cagttttcct tgggctgcaa ggggtagctt gctcattgct ttgcaaaata atggcaaagc 82980catttccaga tattcaggac tgtggagatt tctattctcc attacgataa tgaacaaatt 83040cagataatta ggatctcgag agtatacatt gtgatacgtc aagtcacatt ccacgttagg 83100tgacaaatat acaagtgcat tgagaaaggc agtttcaatt ttttcattag acagcaatct 83160ggtgtagacc cttctaatgg catcaatatc cacagacaca tcatcagggc ctactttctg 83220caaattgttg tctccctgtg agctatcacc tatccttgag gaagatgctt ctgagtcttc 83280ttccatagca gcagcagaac atgcagcttt ttccttttca tcttcatcct tgtcttcatc 83340ttttgcttga agagatttca gttcttcctt ggtatgttgt ttaactttcc ggaagctctg 83400taccaatgcc tcagcactag aaaaaactct tccaataaca cgaattaaag gggaataatc 83460ctctctttct ctacataatt caagaatttc atataccttc tcttctgtta agtaagtcac 83520atctagaaaa tcagaggaaa aaagagaaca tttattttca taatatgcat gtttaccctg 83580ttgcaaaaag ttctaataaa agtaaaacag ccaaacattg tagtctcaac atcatttatg 83640atatacaact ctaaaagtat ctaatactta gattcagcaa acaaaattca atgcttacct 83700atttttttca atgaactacc tacaccaatg acctcaccca tgcttttaca ttctttcaac 83760aatgcataac tcttaatgag atcaggagag tttaaacttt ctttataatc aagatatcta 83820ttaatgtata gctttattta taaatatgaa ccaatatgtc atatactgat taatcataat 83880acagctctta aaacctcact gtagaacaga aatactacta atcttctgag agttttcttg 83940tttcagtact ccgttctcaa aagactgaga aggcaaagtc ttaaaagaaa ggttccttca 84000gcatctatgt atgtattaaa agaaaactgg ttcattttgg gtcttcctag tttgttccag 84060ggtagtcatt ctgtgagtga ttgagaagaa tataccattt ctcatcagct cttgagcaga 84120aactgctttc tgcagccagc aatcaggtta gactaagtct catagagtga cagaaaatag 84180tttttgagca atgaaaacag tgcgaagaag gcaacttatg agctggcaag cactgcagag 84240actatgtagt catcaaatct atttcctctt ttcctgggca cagagttgga ccacatttcc 84300caactttttg agataagtgt tattttcagg ccaatgcttt cccccttctg gctaacagga 84360tgaaagatat tttattttac aggtaaaaac aaatgatata ttttgtgtac tgaccaacat 84420aatactgaga atagtcttaa ttctcccttt catcaataaa gtgacatact tatcatcatc 84480agattaacta cttaaggtgt taattttaga ggcacacaat cctacctcca ttgtgccaaa 84540cataccttta tacatgtact

atgcaacatg tagaattttt aatgactccc acctctgcta 84600taatatatct gtttcacaat gaaagacaat ataaaatgta agtgatcatt gaatggtctc 84660caaaaattga accacaaaaa taccaaattt tatcatacta aggatctgat ctgtggccaa 84720gctacaagag tgtcaacact tgcagtttct tttatgggcc ttaactcttt aagaactaat 84780tctcaggttt gttctattaa gaatatataa gcatcttttg ctaaacatca gtaacctttt 84840ccccaaaagc gtaaaaccac tggaagtcta tttctcatta ttttcattga aaatttttat 84900aataccttct tccaagacaa tgcaaaaccc ctatctaatt tcccaaaatg taacagaaaa 84960ttaagacata aaacactttg cttcctgagc aagtcataag cttaacaaag acttgctttg 85020catgcagtaa tatggcctgt cctagggatc acgattctag actcacatgt cctttgttga 85080tatgtcatgc tttcggaaca tcttcaagtg attctgacag tctactaatc ctatatagaa 85140tgtacctata ctttttccat gtagagtttt ttgttgtggt tgctgttgtt gtttgagatg 85200gagtctcgct ctgtcgccag gctagactgg agtggcacga tctcggctca ctgcaacctc 85260tgcctcctgg attcaagcga ttctcctacc tcagcctccc gagaagctgg gactacaggt 85320gcacaccacc acagccggct aatttttttg tatttttagt agacacgggg tttcaccatg 85380ttgactgggc tggtctcatc tcttgacctc gtgattcgcc cgccttggcc tcccaaagtg 85440ctgggattac aggcatgagc caccatgcct ggcctgtagt gtttaatatg ttaaactgaa 85500tttttcagat atatagataa aaattagtca aaaaacactg taattagata tgaacattca 85560taaagtttgg tttactgata gaggtgttct actgagaata tattaaataa gacatttttt 85620gtaagatttc ttgtgataaa tgtgtccctg aaaatactca atcttttaaa ttctgcactt 85680aaaaaccttt tacacaacaa gagtttggtg cagactactt aatacttacg caactttgtg 85740attagtgtta ataaacataa ttaataatca aaataagagt actagtgcag agaaagcatt 85800tctaatttct aatatatgat agtaactgta aaacactgca aaaacaaaaa caaaaacaaa 85860acagtaaaga acagggaata gtaccagtag cagattagaa agcaggaaaa aggaaaggtc 85920aaaactgcta actaatgcag acttcagcaa aaagaggtgg gggcataaag accggataaa 85980gccacacaat aagcatctct aagatggagc atgtggccaa aagagcagat gagtacattc 86040ctcatgtatc acaccccccc cagctggctg cattccactg tgccagcact ctgcgttcag 86100ttactggtac tctgtggtgc acacttactg gagaactgtg tacaataaag tcaatgcagc 86160ttccacatta tactgacaca ctatatatat agacatttac ctatttttcc acagtgtgtg 86220agctaattca tgaaacagaa ctatacactc tgaagcaaca aagttgtgta aagtgacgaa 86280aataattcta ttgtggaaac agtagaggtt ataacacaat gccacttcat taattctttc 86340aaacttgatt cagatgatgt tactgaaagt tttagaggca aatgcctcta aaattctgtc 86400acttacacgt cataaacaaa aagctttctc tcttaaaata caattgcctc tgcgtgtgta 86460tgggggtggg ggtgcagggg cgctaagccc tggcagtcat tcaaaaattt gattaccgtg 86520ttactaatta cattcactga ggaaaataac ttggagtttt agtattagcg acaaggtaat 86580cgaatcctga attttggaca caggaccatt taaaaaacaa aagacctgag tgaagattgt 86640cattggtagg tcaccaccct tggcagcatg actgtaccaa atgtttgtga ggagagtgag 86700aagcatatat ttgaaggaaa tgtagaattc tcacataact atcacaaaat tttgtcttgc 86760cttctaaaat ggaaaaaacc cactgcttcc tcttcctact tctttcatca catcatcctg 86820aggttccaga actcatgcca gatactgagt ccagttattc tataacttaa atagatgttg 86880tggcctagtt ttggagatta actatcaatt aatacttttt tccctaagaa ttttaaaaag 86940ttcaaatcca tctattaaca aatgaacttt tggcatatga tctgcttcta attagcaacg 87000attaaacctg taaaatgtag ttattattcc tgtcctttac caaaacaaaa aattggttct 87060acggtatcaa aatgtcttaa tgtcacataa gaaaaaacaa ataaaaactc atacatttaa 87120atctcccaca tggttttcag ccaacaattc tcaattaaaa ataaaacatc ttacctttaa 87180aatcaattct agtgcctttc ttgttcattt ttatctcaga gcaggagttg ttgggggcac 87240ctttcgagtt ctcaaggtaa gctgagcttg ctcctttctt ggagggatga ggatcacaga 87300gttttgcatt aatcttataa agctcgaggg ctttaatagc tgctgcatta ttatccatac 87360gaagaaaagt tggacaggaa gcacaaaact cattcgtgca ggcttcattt ccacagccct 87420cagttaactg gtggtagtag cgttctatta gatgctttgc agctgctcgc ttcctgtacc 87480aaacagtcaa acaataagca cagtgattag tatagctgtc atgtacaaag cccaacagat 87540catcaaggca aaagttagtc aagcactgaa gtgattaacc tgtgtgcgaa gatgagaagt 87600agaaaatgga gatgcactat ttaccaacaa cccctgacta gtgaaatagc taataaatat 87660tttctaggat attatgataa agaatgtcaa tgcttactga ctgcattaac agagtataca 87720ctcctttggt ttaaaagggg ggaggggggc tcgtctgatg gtatatgtgc ttaagttgtt 87780tagtacaaca gctgaaaaat caccaggttt acagctaaga gttttaggat tctatcattt 87840taaaaattag cattaggaaa atccagtttt cctttacata atcactattt catttttaac 87900agtttgtaaa catttgactt acagttaaca cagaatatct atctggaata aaaactattt 87960ttcccaatta tcaaccacat gggagactta aaaataaatt tgtaagtgga atatccaaag 88020ctagtgttac actgaaagaa agtggctgca gcctgagtga ggtagatcat gactgtaatc 88080aatcccataa ctgcgggagg ctgaggcacg cagactactt gagcccagga ggtcaacacc 88140agcttgggca acctagtgag gctactctct acaaagtgtt attttttttt aaattagcca 88200ggtgtggtgg catgcactgt agtcccagct acttgggagg ctgaggtgag aggatcactt 88260gagcctgaaa gtcaaggctg ccataagccg aaattctacc actgcactcc agcctgggca 88320agagctagac tttgtctcaa aaaaaaaaaa aaaaaaaaaa aaaaggaaaa taaaaaagaa 88380aaagaggctc caaagtatat aatcttaaat ctaaaacttg tcactaaacc tcctccttag 88440ttccacatta caagatggag tattttaagt tgcatcactc aaaatcttat attcctttta 88500ctctgtgaac ttaaagtaca cagattaatg tcctatttca gctgttacaa gagagctacc 88560aaaactgagg aaggaattat gtgatcaagt aagaagttag atgacattat ctctatattc 88620aacactaaag agtctgtact tctactaact gatgatgaga acaccataat ttcagtagaa 88680taaactactg gatccaaaga aacgtaagtc atagctattc cttttggtcc caaattcaca 88740agtatggaaa taaaaactac gaagaatcaa aattataaga aaaataattc agctaatttt 88800caaaacattc acagaattat cttactaaca tgtaggacta aatgaccaat ggcagagaat 88860ttacatcttg ctaatcataa gaaactcagt aacaagacag caagtaagtg aagagacaaa 88920tgagatccaa ttttgtctca aaagaaagac tttgactgtc taaactatgc aggtaggtga 88980caaaaagtga taattcacac agattcaatt cattctggtg cttatatttt ttcaaatgtt 89040ccatgcaaat ttaccactca ctgcatgcaa acagagaaaa aagaaaaccc acaaagcaca 89100cgtaaaaatt gcataaacat gacttagaga cattttgtta tctatgaagc tataaacaac 89160ccaacctgaa aattaaaaac tgttatcaca gatttatcac tatgcaaaat atgaaagtga 89220aaacatgtaa aaataaaagg ggaatggaga atccagaaac tttaaaacat acattgttag 89280tcttcagtaa agaaagggag ataatgtcat gttctcctaa actacagtga cttccctggt 89340gaaaatgtaa acaggtattt cctttctgga ggaaaatagt gaatatgtat cgaaagtctt 89400aaaaacatga ataccacatg aatgagatcc tacttgtgga aaacaatttg acaattttac 89460aaaaatgcag tttccatatc tgaataaaat caacatgttt aataaaagat tacttaaatg 89520aactgataat aggtcacaca ataaaataaa aactatcaag attgaatctg acaaggaact 89580gcctagtctt cctgatatat cactaactga caaaagtcag ttacaaggta gcaggtgtag 89640gatgaaccca ttgttatttc aaagcattaa tagcattttc tctggacgat gtgattaaat 89700tagttttaaa acttctttca tcttttgatt tcttatttat tctttccata ataaggatgc 89760tacttgcata ttaaaaatta ataactataa ggggaaaaca acagttttaa aacttcatat 89820acatgatttg aatggcccca agatgtatta aattgtctct gatttttaaa aaaattataa 89880aagtcaaaat actaagatag atatttttaa tgtgagcttt tatactaaaa taacttcccc 89940aaaacctgtc agatacatag tacttcttta catactacat aataggagag tgatcagcac 90000aaacagaatg agactgaaaa atcagaagac ctgcattaag tgtcatcctt ctgtccctaa 90060gtagctgtag gatcctaggg aaataactgt ctgggcctca gttttcccag ctttatgagt 90120cccttcagtt ctaattttaa gttattttat gtaataggga ggtgaacaag aaaccagtat 90180ttgttggatg cctgataaca tattacacac actgcggggc actttgacat aggttatctc 90240atttgagtat catgatactg aggattatct tcagtccaca gccaaagaga gaaatagaac 90300tggagtcaaa tcaccaaacc cagatctcaa agaaagtaca caaccaagac aagaatagga 90360gtccaggagt ctttcagctt cagagtctaa gtcattttga ctatactgtt ttgcagaagg 90420tgattcattt ccaaacaatt attttttaac atgagtaata ctgaagaatt ctatggaaaa 90480aaaatctcat aactaatgtg caactgggaa aaaaaaactg aagaatcata atgtgaacct 90540cacatttatg catatatctg ataccacaaa ggtagaccaa atttcttgag gaaatggaac 90600caccacattc tgcaaagcta atttatctct aattttctga ggacctatgt gctatgcatt 90660ttatagacat tctcacgtta gaggggaata aaccttaaca gttaagagct tgcattctga 90720ggccagatgc ccaagttcac atgatggctc tacctcttaa ttaactatgt gaccctgaat 90780gagttacctg acctttctgt acgtcagttt cctcataatt taagtggtag tatctacgag 90840cattctcatg atgagagtat tattattctt cacattatcg attgtgagaa aattaaatgt 90900gttaatatat ataaagtgct cagaacactg cctggcatac taagcactat gaaagtgtta 90960gctactacta ctattgttgt aattgttatc accctcacaa gtgtatgagt tagcactata 91020aaccttcatt ctgctgatgc tcagagaaat tacatcaata agtcattcat ctaataagag 91080atctgaactc agctactaaa tttgagcttt taataactat taagaggagg atatgaaagt 91140agacccctgt atttgatctg cctttctgtg ccaagttaac aaacagcagc tacactgatg 91200agacaggact aaaggaagcc aggtcagaaa ctccatgtta atggtggaat caactatatt 91260aagcattctt ctttttcctc actccaatga caccttaatt tatctgacct catctgtatt 91320actatctaat ttagggtatg taaaaggcat acaaaattta gagcacataa aggggcctct 91380atgttggaaa agtatacatt aaacgacttt ttggtatagt tcctgtgcaa ggttaatcct 91440tgtaccatat ggttcaccaa atcatagctc atgagtcaaa ccaagttcat cacctatttt 91500tgtatgtctc tcaagcttag aatattgtac atattttaat ggctggaaaa agtttaaaaa 91560agagtatttt gtgatgtgaa aattatatga aattcaagtt tcagtgtcca tcaagtgtac 91620tggaacatac ccaagttcat ttgtttataa atatactata tatgatatgc tttggctctg 91680tgtcctcacc caaatctcat cttgaattgt actcccttaa ttctcatgtg ttgtgggagt 91740gaccgggtgg gagataactt gaatcatggg ggcagtttcc cacatgctgt tcttgtggta 91800gtaagtctca cgagatctga cggctttatc gggggtttcc gcttttgcat ccttctcatt 91860ttctcttgtc agtgccatgc aagaagtgcc tgtcaccttc gccatgattc tgaagccttc 91920ccagccatgt ggaactgtaa gtccaattaa acctcttttt tttccccagt ctcaggtatg 91980tctttatcag cagcgtgaaa atggactaat acaatatact atatagttta tggctgtttt 92040tcatactaca atggcagagt tgagtattct aggcctgcaa aacctaaaac aaataccacc 92100tgccttttaa agaaaatgtt tgccaaccct taatttagga tgttatttat ctctgccctc 92160cagtgaccct gccatctaat catttggtct cttaactctc tctttcccct cagcaaataa 92220gtaaaataaa aggtgtacct ttccccaagt attattaaag aaaagaggag aagagggaga 92280gagggaaaga gggaaaaaga aagtaaggaa aaggagagga tgcaaaagaa aaagaaggaa 92340agaaaggaat ttccctgatg aggtcccttt agcaacacct tatcagctgc tcataatcaa 92400aattcctgga agaggaacta tagctttact tgccataatt tcttcacatt accttgactt 92460ttctttttac tcaaaaaagt atgttataat gtgttgattt ttaaaataaa aaaaactttt 92520cttagtcagc aatgaaggtc aagcgagacc ttctagtaag tcttgtaaag ttagacacat 92580gaaaatactt atccatttca aacagacaat actttccaat ttttatttct atgactgaat 92640tagtgggaca gtgctacaaa cagaagtcct atggaataag tcatatttgg aaggtcagtt 92700taccctagac aacttgacag caatcagttt aaaaatggtt tcacctagag ggctgtgtac 92760tgatgttagc aattaacttt gaaaggcata aaaattaaga tggactgatg atacaaggat 92820ggttataaga taaagcaatg taaatattaa tggcagaatc taggtgacag gcatatgcgt 92880gtcagctata aaatctgtgt tcaacatttt ttagttccca catatcagtg agaacatgtg 92940atatttgtct ttctgtgtct ggcttagttc actaaaaaaa tgaatttaca ggacaattgg 93000aatataagca atgaatacaa gcactaagaa gatgaggcca tagacacagc tgccagaaag 93060tgaagatgtg acagtgaaca ctgaacaaat aagattagaa ttacaattag aatcagaaat 93120gacaaaagag aaatatgaac tgtgagatgc aatataaaaa ctgatggtga gtaaagcaaa 93180gaataaaaaa taaaaaggga aagattaaaa ggaaaacgtg actataagta gagtgacctt 93240tatatttaca gaaggtacag cagaatgaac atgtgagaag tctaaaaaac agatataaaa 93300tagaaacaac tcaaggctta tataattagt ctataaagct aagaaacact gcttagtaat 93360ttctcgtaag tggagcctct ttattctttc tgggatgttg caaaactcca ttccatctca 93420aagttatttt taatgtaaaa atttgtaaga gtgagcaatg gtatcctaaa tagttctagg 93480ccttgtagaa atagattgta agagacatct gtgacatagc tatattcagt acgatgtagg 93540gtctgttaca atgggatgaa atgaatttag attatttcaa gactaaacct acaattgtga 93600actctttagt accatgaacc aaacaaaaac ctattaaata tagaacaaga cgattttcat 93660gaaaaaataa acacaaacaa atcagtattt tcaagtgttt gaaatatagg ttaaccttta 93720agaactgttt tattcatttt tatagtctca atgtccagaa tgcttgatat atgattaaaa 93780aaaagtaaaa ttatacaact aaatatttat ataattcatc tgtaaaatga aaatctgttt 93840taacgtgtca tatttcacag ttaggagaat aaaaacctca aatcaacaaa ttaactttac 93900atcttataga attaggaaat gaagaacaaa ttagactcaa acttaccaaa aaaaaaaaaa 93960aaaaaggaaa taacaaatta cagttaaaca aaatagagaa aaatagaaaa tttttgcaat 94020agaaaaaaaa aaatcaacaa aattaggagt agctgtttca ttgaaaagat caacaaaatt 94080ggcaaattct tagactaaga aatgaagaga atagacacaa acagcaaaaa ttggaaatta 94140gaaaggagat acaacaactg ctgccgcaga aataaatagg accataaaag actaatatga 94200acaactatgc cccaacaaat ttgataacct ctgagaatgg atagacacat caaacccacc 94260aagactgagt cacaaataca tacaacttct gaatgtacct ataactatca gagattgaat 94320cagtaactgt ccaacaatac cagggcttca ctgaaaactc taccaaacat tactctttga 94380aaaaactaaa gaggacagaa cactttcaaa atcattatga ttttgataat catagtaatg 94440ctaagtaagc attacttaga tatcaaaatc agacaaattt actacaagaa aaaagaatcc 94500ctaataaata ctgatgcaaa aaaaatcaac aaaataccaa gaaacagaat tcaacaatgc 94560attaaaaaga acatcctttt gtgatttaaa aaaaggccag atatggtggc ttgtgcctgt 94620aatcccagca ctttaggagg gcaaggtggg cggatcacct gaggtcagga gtgtgagacc 94680agcctggcca cacagtgaaa ccccatccct actaaaaata caaaagctag ctacgcacgg 94740tggtgggcac ctgtaatctc agctactcgg gagactgagg caggagaatc acttgaaccc 94800gggaggcaga gctggcagtg agctgagatt gcatcattgc actccagcct gggtgacaag 94860agcaagattc tgtctcaaaa ataaaaacaa aaacactcaa tttgctagaa agatatggaa 94920attacttcaa aataatgaag gccatatata gaaagaccac agctaatgtt attcaatgat 94980gaaacaccga aagttttccc tctaagatca caaacggaac aagaatgcac acattcacca 95040cttctactca acatactatc agaagtcctg ccagagtcag caattgggca agaaaaagaa 95100ataaaaggta ttaaatgaga aaagtaagaa gtaaaattac ttctgttagc aaatgacatg 95160atcttatacg ttgaaaactc tagcccacca aaaagaaaaa aaactgttag aataaaggat 95220acaggatata aaaataaagt tacaggatat aataataaag ttacaggata taaaaaccaa 95280cattaagagt tgcatttcta aatactaaaa atgaagaatc caaaaaggaa attaattttt 95340aaaatccatt taaaatgaaa tcaaagagta aaatacttag ggaatatttt caacaaggaa 95400atgaaagaca catacattga aaattataca acataggcca ggcgcagtgg ctcacgcctg 95460taatcccagc agtttgggag gccaaggtgg ggggatcacg aggtcaagag attgagacca 95520tcctggccaa catggtgaaa tcctgtctct actaaaaata caaaataaat tagccgggct 95580tggtggcaca cgcctataat cccagcaact cgggaggctg aggcaggagt atcacttgaa 95640tccgggaggc agaggttgca gtgagccgag atcgcgccac tccactccag cctggtgaca 95700gagtgagact ctgtcccttc tcccccgccc aaaaaaaaaa aaattataca acaatgctga 95760aagaaattga agataatata aataaatgga aagacatcca agtttatgaa ttggaagatt 95820tagtattgtt aagatggcaa tactactgaa agcaatctgc agattcaaag caatccctat 95880caaaatccca atggcatttt ctacagaaat agaaaaaaaa aaaaatctta aaattattac 95940ggaatcttga aggaccctga atagccgtaa caatctggac aaaaaacaaa gctagaggcc 96000tcccattccc tgatttctaa agatattgca aagctacagt aatcaaaaca ctatggtgct 96060ggcataaaga cagatataca gaccaactga gcagaagaga gaacacagaa atagggaaaa 96120gaggttcttc aacaaactgt gttgggaaat ctgaaatcta acatgcaaaa gaatgaagtt 96180ggagccttat cttacaccat atacaaaaat aagctcaaga tggattacag acctaaactt 96240acaacatcaa actataaaac tactacagaa aacatagaga aaaagcttca tgacattgga 96300cttggcaatg atttcttgaa tagatcacca aaagcacaag caaccaaagc aaaaatagac 96360aaaccgggga tacataaact caaaaatttc tgcaaagcaa acagtaaaca gagtaaaatg 96420gcaacctaca aaacaggaga aaatatttcc ataccatgta tctgttattt ggttaatatt 96480cagaatatac aagtaactcc tacaattcaa caacaaaaaa tctagttaaa aagtagacaa 96540aagacttgaa tagtcatttt tccaaagaat gtatacaaat ggccaataag cccatgaaaa 96600gatgctcaac atcactgatc agtagggaaa tgtgaatcta ccatctgaaa ccattaggat 96660gaccaccatc aaaaaaacag aaaataagtg ttggtgaaga tatggagaaa ctggaaccct 96720tacgcaccat tggtgaaaac gtaaaatggt gccaggcact atgaaaacaa catgaagatc 96780cctcaaaaaa tttaaaatag aactacctcc agttccacat ataaagagct tggaagtcac 96840cactccattc taacaggtaa aaaatgacaa actgaaaaac aatcaaatct gagataggat 96900aagtgaggtc acagggcaaa taactgcctg taagtgagag cgaaggacag gtgaacacat 96960agcatcacca tttatctgag atgaaaaaca caagcagaaa cctccacagg gtaggaaatc 97020ctgaagtgta atggacaaat tgatgaaggc tcagtgtgga caactccgaa agctaaaaac 97080tccacagtta cccaattata gggggacccc tacaattttc taagttttac atccaggcaa 97140ttgaccagct tctcacagtc aatataggag aaaaattctc aaccctcagc aggggagaca 97200aagagaaacg attttcaagt acaccacagt attctggtct taacaaggtt tgtcctcagg 97260agaaactaac cagagcctga cctgctgatg ttgtagcaaa gccaaaatga tgtgggggaa 97320gagaaataac ccaactccac gccattatag ctatcttgtt ctatctaagg gaatgggggg 97380gtggtgtggg actgagaaac acttgtgaag ttcacagtcc agaggcataa gctcactaaa 97440agactgacct aatcataaga ctacagaact cttctcctct tcctacacct tacaaccaca 97500ttactaaagg ttcatttaca acagttcctt ttacctagta catcgtgtcc aagctatcaa 97560taaaaaaatt acagggcata ataaaaggta aaaaacaagc atcaggacca aattcagcag 97620ggatttggaa ttatcaaact gggaatctaa aacaaccatg attaatatgc taagggccct 97680aacgaattga ctaaacaaca cgttaaaaaa aaaaaaaagg agagatgaaa atcctaagaa 97740tgaaccaaaa ggaaatgcta gagataatac aaaaacaaaa acaaaaaaca gaacactgta 97800agagaaatga agaatgcctt tgatgggctt attagtaggc tgggcatgga tgaagaatct 97860ctgagcttga aaatacatgc caaaaaaact acatctgggc ataaaacgtt gttaactaca 97920gaaattcaag gataagaaaa aaatctgaag taagccagag ggaaaaaaat cactttaccc 97980atagaggaaa aaaagaaaaa aaaagaaaaa atacatgtga cttcctacaa gaaaaagagt 98040ggggtgaaat atttacagtg ttgagagaaa aaattcataa acgaagcatt ctaccttgtg 98100agattattac tcaaaagtta actggaaatc tacatataca gagcataaaa agaaggaaac 98160acggaaagta aaataaaacc ttttattttt cttatactta attatctaac aaataagaat 98220ttgttcaaaa taatgtcaac aattgtgctt acgcatacag acgctcctaa acttacttat 98280tgggttgtgt cccgctaatg ccattataaa gtgaaaatat cacgaaaaca caacttatcc 98340aaatttgtgg gatacagata cagtgaaagc agtacttaga aagaaacata caacactgat 98400gcatttatta gaaaagaatg atctaggcca ggcgtggtgg ctcacgcctg taatcccatc 98460actctggggg gctgaggtgg gtggatcacg aggtctggag ttcaagacca tcctggctaa 98520cacactgaaa ccccgtctct actaaaaata caaaaaaaaa aatcagccgg gcgcggtggc 98580aggtgcctgt agtcccatct actcgggagg ctgaggcagg agaatggtgt gaacccggga 98640ggcagagctt gcagtgagcc gagattgtgc cactgcactt aagcctgggc aataaagcgt 98700gactccgtct caaaaaaaaa aaaaaaaaaa aagaaacaaa aaaacaaata agaaagaaag 98760aaaagaatga tctaaaaatc aattatctga gctaccacct tatgaaacta gaagaagcaa 98820attaaatcca aacagaagta ataacaatta gagcagaaat caatgaaatt gaaaacaaga 98880gaaaaatcaa agaaatcaaa aactggttct ctgaaaagat caacaaaatc aataagcctc 98940tagtcaggcc aactaagaaa aagagacaaa ggacaaaata actaatatca gaatgaaaag 99000agtacacatc attacagatc tcatgggcat taaaaggata atcaagggat attatgaaca 99060actttatggc cacaaatttg gtaacctaga cgaaatgaat caatttctta aaagacacaa 99120tctattagaa ctctaagaag aaacaatctt gattaaaaag catatattat agaaattaaa 99180catgtaatta ataatgttct aaaagagaag gcatcagacc cagatggagt ctctgatgaa 99240ttctactaaa catttaagga agaaaccaca ccaattcttt acaatctctt tcagaagaca 99300gaagcaaagg ggatgctcct atctcattgt atgaagtcgg tcagcattac tctaataccc 99360aaaccagaca gacattatca gaaaactaca gaccaatatt tgtcatgaac aaagatgcaa 99420aaattgtaaa caaagtatta gcaaactaaa aacaacaaat ttctccacca gcacaaagta 99480ggatgtatcc caggtatgca agactggttc aatattggaa aatcaattaa tgcaattcac 99540cgtatcaaca gattaaagaa aaaaaaatca catgaccata ccaatagatg gagagaaata 99600tatgacaaaa tttaacaccc

actcatgata gaaactagta aactaggaat agagggaaca 99660ccttcaactt gataaagaac gtttacaaaa aacatacagc taacatcatc tttaatgatg 99720agaaactaga aactttctca ctaagatcag gaacaagaca ggaatatccc cacctcacca 99780cttcatactg gaagtcctag ctaatacagt aagacaagaa aaggaaataa agggtatact 99840aactgggaac aacaacaaca aaaatgcctt tgtttgtaga tgaaatgact gtatgtgtag 99900aacagataca ggaatttgaa aaaaagaaaa acaacaaaaa cctcctggaa ctaataagtg 99960attacagcaa agctgcagga tgcaaggctc atataaaaaa gttaatagct ttccggtaaa 100020ccagcaacaa ataagttgaa tttgaaatta aaaacacaat accattttac attagtgtgt 100080cccaaaatga aatacttaag tatagatcta acaatacaag gaaaaatttt ttagaactct 100140gatgaaagaa atcaaagaac taaataagga agagctattc catgacccag aagagccaac 100200tcaatattga cagaaaaagt cagatggaca ctactgactt caagatttac tgtaaagctc 100260cagttgtcaa gatggtgtga tactgacaaa agaactgaca aaacagatca atgaaataga 100320atagagagcc cagaaatgga cccacatagt caaaggagca atggcagtat ttgacaaaga 100380ggcaaaggca atacaacgaa gaaaaaaaaa atagtctttt tcaataaaca gtggtaataa 100440ctgaacatcc acaagcaaaa aaaaaaaaaa aaaaaagagt ctagacacag gtcttacacc 100500ctttacaata ataaactcaa aaataagcca gagaattaaa ggtaaaactc aaaactataa 100560aactcctaga ggataacata ggagaaaatc tagatcttgg gtttggtgat gactttttag 100620atacggcaac aaaggtacca accatgaaaa aaagaactga taagctagac ttcatcaaaa 100680ttgtctactt gtttcttaaa aattgttgtg tcttttgcaa aacacaatgt caagagtatg 100740aaaagacaag ccacagacag ggaggaaata cctgcaaaag acacatctga taaagaacta 100800ttaactaaaa tgtgtaagga agttgggcgt ggtggcttac atcacagcac tttgggaggc 100860caaggtgggt ggatcacctg aggtcaggag gtcgagaata gcctggctaa catagtgaaa 100920acccatctct actaaaaata caaaaattag ccaggtgtgg tggtacacgc ctataatccc 100980agctatttgg gaggctgagg caggagaatc acttgaaccc aggtggcaga ggttgcagtg 101040agccgagact gcgccactac actgcagcct gggtgacaga gcaagggtcc gtctcaatag 101100atagacagac agacagagag acagatatag tgtaaggacc tattaaaatt ccacaagaaa 101160acaaacaacc caattagaaa atgggacagc caccttaaca gacacctcac caaagatacg 101220cagatgtcaa ttcagcatat gaaaagatgc ctcacacatc atacgacgcc agagaaacat 101280aaatcaaaat tataagatac acacctatca gaatggccaa aattcagaac agtgataaca 101340ccaaatactg gtgaagatgt agagcaacag aaacactcgc tcattactgg tgaaaaatgt 101400aaaaggatag agtcacatta gaagacagtt tggacatttc ctacaaaact aaacattttt 101460gccataagat ccagcaaatg cactccccgg tatttacaca aagtcactga aaacctaagt 101520ccatacaaaa acctgcacac cgatatttac agcagtttta ttcataatta gcaaaatgtg 101580gaagcaacca caacaatgtc cttcattagg taaatattca ttcacaaact gtgatatgtc 101640cagaaaatgg aaaaagagag gtactaacaa cctatgaaga catggaagaa actttaatcc 101700atattagtaa gtaaaaatag tcatgatgaa aaggctatat actgcatgat tacaagtata 101760taatatgctg gaaaaggtaa agctacagac acagtaaaag gattggtgac tgccaaggag 101820tgggagggaa cgaaagataa acaggcagaa cacagaggat tttggggcag tgaaaacatt 101880ctgtatgata ctataatggt ggatacatgt cattatactt tgttcaatgc cacagaatgt 101940acagcactaa gaacgatccc taatataaac tacggacttt gggtaattat gatatatcaa 102000tataggttca ttggaaagac atgtaccact ctgatgggag atggaccttg actatgagag 102060aatctataca tgtgtggaag gaggtccagg gaaaatctct gtaccttcct ctcaattctg 102120tgaacctaga actcctgtaa aaaaaaaaaa aaaaaatttt ttttttttta aatagaacac 102180atatgatcca gtaatccaac acctgggtat actcccaaat gtgttaaaaa caggttctca 102240aaaaaatatt tgtacaccca tgtttatttc aacattacaa cattcaaaag tggaagaaac 102300ctaaacatcc atcaacaaat ggttaaagaa aatgtgatat acatatgtaa caaaatgtta 102360ttcagcctaa aaaaaaaaaa aaaaaaaaga aagaaatcct gtaatatgct acaacacaaa 102420tgaaccttga ggacattatg ctatgtgaaa tagtacagtc aacaaaagac aaatacttaa 102480tgattccact tatatgaagt aactaaaata gtcaaatttt agaggcaaaa agtagaatgg 102540tatttgccaa ggaataggaa gaagggggaa aagaagagtt gttcaatgaa tgtagtttct 102600gttttacacg atgaaaaagt tctagagatc tgctgcacaa cagtgtacac acagttaacc 102660acaattgtac tatacacttc aaatcattaa tatcatccag tttgttgttt ttgacaattt 102720ttaaaaatct accccatttt tttctttacc tcttatataa actgataaaa acctgtttta 102780tcctaatcaa ttatttcatg actactgctc cccagagaaa aaatgacatg tgaaatacta 102840cgacttatcc atctcctggg tctgagcagt acatgtcagg aacaagaggg aactgtagct 102900ggaagacagt agaggagccg taggacaagt ctcaaagaga aggaagaaaa ggcgcaaaga 102960aagaaaacaa gtttaagata gtaggaaagc attcaaagac tgcaacctga gataaggggt 103020tttcactaaa ggttttgggg taaaacagtt tgagttggca tgcctgtaga catgggaagc 103080agcagtgatg cagaggtgaa cattactgta gataaaacag atgggaattt gagttgctag 103140ggagtgaggg gttcatcaga taaataaaga tggcagcaga tttgggcaag agtgaagaag 103200agtaagttca ggtatcagtc ttcaaaaacc gagaagaaac tgagagctca ctaaaggcag 103260atagaggatg acacaaccag aaggcataag cttcaaagga gcagggattt ttatgctgag 103320agagtaacag gaatagttca aagcagcagt ggagaacacg aataacattc tgtgtgggag 103380aagaacacgt ccagagctta ctgtacctat aacctagctt ctgtaactgg aaatgaagaa 103440tctgctctag acaggtcatc acttttcact acttatttta cttggccttc ctaagaaata 103500tagacctttt ggaaattatt gacttttggg tctaggacac tatttcttgg tttcctccct 103560tctctctggc tgtttttcag agacttcgct tccaaatcta gagacatttg tattctccag 103620ggatcagcta ttagatactt tctcttttaa atttcccttt ggacagtttc atcttctttc 103680aaggattgag caataactaa atgacttcca aatgtctact tcagtataaa ttctgcttgt 103740caggctacat aaaatgggct cttccaggaa ttataatgtt taaagaaaga gaaagccacg 103800tgatttcagt atgacagaga tgactacaga ggaatagcaa taaaaagaga cgcaacttag 103860cagaggtgaa tcacaaaaga tgactaggag ctgatgacac taagggtggg aagaatgttt 103920cagatagagg aaaatgagta aaagcacagg gacatgagaa catggtatta taagacaatt 103980ccaagcactt caagagccta aagttcaaga aaagtttcaa aaatatgaaa atggagaggc 104040agggagaagc tagatcatgg aagacctcac tagccatgat aagtaattct ttttttttag 104100ccaaaagagg agtaatctaa taaaagctaa agatcttctc ctcataaaaa cacatgcaca 104160cttcaggagt gcacatgcag tcttccggaa gattcgtaac ccactatatt cataaaccat 104220tagagaagaa ctaaaacaca aggagtcact gaagggtttt aagcagctaa gtggctgata 104280aaatttgcct ttccaataac tgaaaccaaa gtgggcaaaa ctggaggcaa gcaatccagt 104340taggtaatga ctactgtaat agtccagaca aagaatgtag tagtagcaag aaaaaaaaag 104400atgaaattga cccaggaagt atttaaaata agtaatttgt aagaggtaaa taagctggaa 104460aagctagaga taatgggtaa ggtttctggt atgggcaaca agatatgcta caagctgaga 104520tgttaataaa aacaaaagtt catcagcatg tagtaattta tttatattat gtcactgaat 104580attcaattat gttaagaggc agacagtagt taaaaacaaa gaactggtga atcaaaaagt 104640tctgctaatt tggggaaata gcatcccttt tattttcatt aatctctttt ggctttgatc 104700ctcatttgta gctgacaaga agttctatgt attaaactat tctcattaat gtgtccaaaa 104760atctcttggg aataattaag acataaatta attcaaaatt cacatgatta caaaaatctc 104820tccttagata atctcataat atagccatgt tttgctcact ctactttgag aagattcagc 104880cagaagcagt tcatccattc aagtatgttc ccttacattt gaaacagaag atacaggttt 104940atatgatgct tcctgtggca gaaaagcagg gcagactgtg actcgtgtca ctcccacacc 105000cgatgccttt catgggctcc tgtgtctgtc agaagaaagc aaagggcctt aatacggtcc 105060tggctttcta catccaaatg cattttccac acctcccatc agctaactct acattacggc 105120aatacagaac ttcttactac tcagtggact tgctctgctg tcttctctga ttacaggtgt 105180tattcttcct gcccagaaca cattattctt agttatttcc aatcatcctc aaggtttcag 105240cttatatatc acttttaaga tgattaggta ttcttcctct gtagtctcat agcaaccagt 105300acctattctc actattatat gttgttgtaa ctgactatca attaaatggg aggctgaggc 105360atatgaccca ataacttatt tactgttgta tcctctgaac ctagcaaatt acagatcctc 105420aatagagatt tttcaatttc tcttctgata ctgtctagat aacactgtca attaaaccta 105480ccttcctcta attattcgac cttgaaggga aatacatgtc agaatacctc ctggtttctt 105540cagcatatca gaagcataca aaaggacctt ctcaatccag agaccaagaa tatgatatca 105600gaacctatag aaaaggattc tgaaaatacg agaaccactt atttgattga agagatttac 105660ttcggaaaat atttcaagaa gttacttgtc ccaaaattct catagatgtg taagtacacc 105720gaagttagaa gtcatatagg caataaaagc atccaagctc tatttccatc atctactctg 105780attaaaaggc tcaactctcc tatcaaattt gagataatca tgtggattat gcagcacagt 105840gacttgctac atgttcaatg ctcaaaaaga tgataggtgt tattagccac atcctacggg 105900ctgcgtacat acttattagt ttatatatat acataaactg atcctaaaaa attcttccta 105960catttctgca agagaattaa aaaggcagaa acaaacattt tgcataaatt tactagcttc 106020attctttcat gtaggaaata gttactgact accttctgta tgccaggcac tgtcattagg 106080cttccattct agtggtgaag caaaacaaca aagaaatcaa tatgcaagac gtcaaatgac 106140attaaatccc acgaagaaaa atgcagcaag gtaaggggaa tgcagaagat tgaacctgat 106200cattctttga tgcagctggt caggaaaagc atcatttgag cagagagctc tttctgaatc 106260aagtgagcaa ggaagccaca tggattatca cagagaacgg tcgcagggtc actgacaata 106320gttatgagac ttaagatgac aaggatacag tcactgtaga cagagataag caagtaaggt 106380agagagatca cagaagtgaa aggaagccag ttcatacagg gccatgtaaa tctccagatt 106440tcacttttaa gatgggaagc ctctgaaaag ttctgagcat aataaataac acggtttaac 106500tcagatttta aaaatatcat tctggctgct actagagaat acactgtaaa gtgtaagact 106560cctgggaaac aatttctttc ttttgccaaa atatgatgcc tttaaacagg gtaaacagaa 106620gtataatggt taaaaaaaaa catgaggcat gggtattttg aaggtgtagc caacaagatt 106680taccaataat ttgagtatgt gacatagaga aattaaaaga tgcttccaag gcttttggtt 106740gcacaggagg gaaaaaaagt tgttactaac tgagacggca aaagatgatg agagacgtag 106800gtttgggagg atgaaaatca ggagctccac ttaggacatg ttaagtttga gaatgagact 106860taactgttgg ataaatgagt ctacagttca gagggcaaga gttggtgatg gaaataggag 106920agttgtcaaa gcgtagatta aaagctctaa cctagagaga aagaataaaa acgtccccag 106980gactgggtcc tggggcattt ctgacattta cagtaaactg aacatttatt aaaactaact 107040tgctttactg aaccataaat cacttgctgt atttacccct gctccattta tagttataca 107100cctgaaaaca tagccttagt gttagaggta aagagtaaat accaaacata aaaaatccaa 107160tctggtaggg tgcggtggct cacgcctgta atcccagcgc tctggaaggc cgaggcaggc 107220agatcacgag gtcaggagtt tgagaccagc ctggccaata cgggcaaact ccaaaaaaaa 107280aaatctgaag ttactgagaa actgatatga aatcattaag aaaattaagg ggtctaaatt 107340aatacataca acaaagagat cccacaacat ggtttttata aatttaaaca aaatccatgg 107400ccaggcacgg tggctcacgt ctgtaatccc agcactttgt gaggccaagg caggaggatt 107460actttggggc caagacttca agaccagcct gggcaacata gtgagaccct gtctctacag 107520aaaaaaaaaa aaaaaaatta atgttttagc ctggcatggt ggcatgtgcc tgtagtccca 107580gctactcagg aggctgaggc acgaggatca cttgagccca ggaattcaaa gttgtagtga 107640gctctgatca caccactgca ctccagctct gtcacttatg acaaagcaag acactgtctc 107700aaacaacaag caaacaaaac aaaaaactaa agtaaatcca ggtaaaataa acatacaaat 107760aaatgaaaag gtctgggtta ttgcattagt tctgtttgta ggcttaacta caaaatggtg 107820tccagttgtt aaaaatgttt gcttaggagt attgattttt tttattatgc tcttctacat 107880tctaggaaat tggcaacatt aagaattcct aaaaacaaac aggagtgtta agtgtataaa 107940aattattatt taagatgcat tcaaggcatt ccttagttaa attaaaatat caaatgcatg 108000tcaaattaca cacaaaaaaa tcatgcttca acaaaagtgg gatgttgcca ttaatggaca 108060gttagttctc aggtaaaacc aagaatcctt cattacctat tctgtaaatt aggacaaata 108120ctattaaggt attaggttgg tacaaaagta attgaacaaa cccagaccta ctcacctcac 108180tgaaagtgct gcctggctat agtctctaga tacaggaatg tgagaaatgg tagcagcctt 108240gaaggtttag agctgtattc tcataccaat tatggctaga aacttaaaca gctctactga 108300gacattatgg gaggtctaat ttcctgttac tctactttaa cacagccaag taaaacagta 108360agaaagcttg cttaatttga caaggactga atgccagagt gtcacctgga aacttgtgac 108420aaataatcat gcctcacaca ttcatccttg gtttttacta ccaaatgcaa gcttcatcta 108480tcctcttcca ttctaaactt gactcatcct cctaacctct tccagaaaga tctctagaaa 108540cccagagtct ttctgaagac ccacaggctg tcccctatct tcaactactt tacataatgg 108600cccctcaact tcttagcttt cacgaaagcc tggctcttcc ctgaaggcac ggcctatcat 108660gcaacccttt tatgtgactc ctaaaaccca agtccaccat tattcttcta atactctgta 108720ttaaaaataa acaacaaaat tccctgtatt aaaagcaaac aacaacaaaa aacttgtaat 108780ttgaaactca cgtcatttaa ctgaatgcct tctctttctc tctgttctgg tcaactcaac 108840ccacatattt aagcctccac tttgccccca gttacatcat cagcaaagtg acttcaatgt 108900ccctgtacat ggttctatcc caatactcta ggcccccaaa atatctgcct ttctaaattt 108960gagatatctt cacctcctca tttcagcaaa tcactccctt gtctttgtta gacatattaa 109020aacctctgat aatgatcatc tcagctatct ttataacagt ttcactcata ctcatatctc 109080ctttcaccta tcaagacacc aggtttggtg acaaataaaa atgatacata tttgggaggc 109140tgaggcagga gaatcatttg aacccaggag gcagagaatg cagtgagcca agatcatgca 109200tgctactaca ctccagcctg ggcgacaaag caagactcca tctcaaaaaa aaaaaaaaaa 109260aaaaaaagat acgtacttat tgtatacaca tgatattcca aaatatgtat acattgtgga 109320atggctagat caagctaatt aatacatgca atacctcaca tacttacatt ttacggtaga 109380acatttaaaa tttactctta gtgatttcca agaatacact gttactaact agtttccatg 109440ttgtacaaca gataccttga acaaattcct cttaattgac caacatctct gcaacccagt 109500ccatccaccc ccagcccctg gtaaccacca ttttacatct ctgagttaag attccacatt 109560taagagagat gtagtatttg cctgcctttg tgtggctggc ttactttaca caatgccctc 109620caggttcatc cctgctgtca cagatgacag catttcctcc ttttgatggc tgaagtgtat 109680tccactgtgt atctatacca cattttcttt atccatgaac gcgtaggctg atgctgtatc 109740atgggtattg caaacaacac tgaaaggaac acgggagtac agatctcttc agcacactga 109800tttcattctt tgatacatat atatatccaa gtgtgactgc tgaatcatat ggtagttcta 109860ttttttaatt tctaagcaaa cttcatgcta ctttccataa atgctgtact aatttacatt 109920cctaccatgt gcaagatatc tccaaatcct catcatactt ttaacagcca ttctaatggc 109980catttgaatg tcttattttg agaaatgtct attcaagtcc tttgtccatt tcttaactga 110040ggtgcttact attgagattt ctgagttccc tatatatttt gcatattaat cccttaacag 110100atatatggtt tgcagatact atcttccatt ctatgagttg ttttttcatt ccgtagattt 110160tttctttttg ccatgcagaa gctttttagt ttgatgtgat ctcattcatc ttttattttt 110220gttgcctgtg ctttggggtc atatccaaaa aaatcactgt ccacaccaat gtcatgtaca 110280tttctcctat attctggtag ttctacagta acagctctta cttttaagac cttaatccat 110340tttaacttga atttggtata tgatgtgaca tacaggtctg atttcattct cctgcatgta 110400gatattccgt tttcccaaca tcacctattg aacagacttc cctttccctg ctgtgttctt 110460tgcaccttgt ccaaaatcaa ctgacagtac gcgaatttat ttctgtgctc tccattctgt 110520tctatgggtc gatgtgtctg tttgtttctt ttgttttgtt tttaaaagac agggtcttac 110580tctgtcatcc aggctgcagc gcagtgatgc aatcacagtt cactgtaacc ttgagctcct 110640gagctcaagc aatcctcctc cttcagcctc tcaggtagct aggattacag atacacagca 110700ccatgcctgg cttaaaaaaa attttttttt tgtagagaca tggtctcact atgttgccca 110760ggctgctctc aaactcctgg tttcaagtaa tccttctgcc ttagcctccc cgagtgctgg 110820gatacatgtg tgagcaactg tgcccagcct gcgtgtctac ttctatgtga ctaccatgct 110880gttgtaatta ctgtagcttt gtactatatt ttgaaatcag atagtgtgag gtctccagct 110940ttgttctttt tactcatgat agctttggct atttaggatc ttacgtggtt ccatataaat 111000tttggcactg ttttctagtt ctgtgaaatt taacattgga attttgataa ggattgcatt 111060aaaactgtag attgctttgg ataatagaaa cattttacaa tattaattct tccaatccat 111120aaacacaaga taactttgtt tatttgtatc ttcttctatt tctttaatca atgctacata 111180gttttcaact atttggttaa agttatttct aagtacaatt atccctcggt atctgcagga 111240tactggttcc aggatctccc atagatacca aaatccatgg atgctcaagt ccattatata 111300aaatggtata ccatttgcat acaacctata cacatcctct tgtatgcttt aaatcattac 111360tcagtaccta atacaacata aatgctacgt aaatagttgc tatactgtag ttttcttatt 111420gtataagaaa acagtatttt ttattgttgt attttttttc tcaaacattt tcatccaaat 111480gtggattcaa tttgcaaatg cagaaccacc gatactgggt gttttgtttt tggtacctat 111540cataaatgga attgtcttct tgatttctct tttggataat ttgtgattaa tgtacagcaa 111600ttctactgat ttttgtaact tgattttgta tctcacaact ttactgaatt tgtttatcag 111660ttctgacagt tctctggtgg agtctttagg gttttctata tacaagatca tgtcatcttc 111720aaacaggtgc aacaacatct tcctttccta ctgggatgcc ttttctttct cttgccgaac 111780tgctctcaca aggacttcca gtactgtgtt gaacagaaat ggcaagagtg ggcattctcg 111840ttttcttccc aatctcaaaa gaaaagcttt caacttttca acattcagta taatgctagc 111900agtggatttg tcaatatggc ctctatttta tagaggtata ttccttctat atttaatttg 111960tgagagtttt tagcacaaga gaaagctgaa ttctgtcaaa tgctttttct caattatctc 112020aattatctat tgagatgatc atatggtttt ggcctttgtt ctcctaatgt ggtgtatcac 112080atccttgtac ccttgcggta aatcccatgt gatcacagta aatcatcctt ttaatacact 112140gttgaattta gttattattt tcttcagaat ttctgcatct aaattaagaa tacaggtctg 112200taattttctt atcctggaga gttgtttggc tttggtatcc aggtaatgtt catctcccaa 112260aatgagtttg aaagtattcc cccttcttaa atttctcata aaattttgag aacaatttgt 112320actagttctt ctttaaatgt ctgctagaat tcagcagtaa agtcaccaag tcttgggctt 112380cctttggtgg gaagtttata caggaaggat tatataggaa ggattataac tgatacaatc 112440cttgtcactg ttcaaatttt taatttcatg attaagcctg ggcaagttgc atgtgtctag 112500gaaattatcc gtttcttcta tggtaatcag tttgttggca tataattgtc ccgtctctta 112560tgatcctttt atttcttgta cttctctggt atcagctgca atgggtcctc tttcatttct 112620gatttaactt gagtcatctc tttttttctt agtctagcta aagacttgtt gcttttatca 112680tcaaaaaaca taactcttaa tttttttgat cgagttacct ttttccttag tctagccaaa 112740taacctgtct tttaagttca gattcttttg tctgtttgct caattctgct tttaatgctc 112800ttttgcattt ttcattcaat ttattgcatt ttcagcttca gaatttgttt tttacaaaat 112860aatttcaatc ttgaaatttc tcattttgat tgttttcctg attttgttta attgtttatc 112920tgcattttct tgaaattcat tgatcttcct taaacaacta ccttgaattc tttgtcaggc 112980agttcatata tctccattta tttacgggtg gttcaggcgc cttattgtgt tcctttgact 113040ggtgtcacat ttccctgact gttcttgatc cttatagtca tgtgttggca cctgcacatt 113100tgaagaagct gagacttgtt ccagtctttg cagagtgacg ttatcgggga aagtccttca 113160ccagtcggcc tgtccagaga tcatgcagcc tgagtccaca ggggctggcc tggcatcctg 113220gtctactaga gtgagcctga accttgggtc tgcaaggacc agtgtgaagg ctgggtaggt 113280gggtgttggc ctagaatttg gatcacaagg ggctggccaa gtactgggtc tgaacccttg 113340ttctgcaggg atgtgaggcc acaggggccg atctagaggg cagggctaca gtgaccagcc 113400tagcactaga caggcttgga gtctagggct atgggatcca gcgtggtgcc ggggtgggcc 113460tgtaggcttg gtttgtgggt attggcctga agtctgggac catgggggcc cgcctagcac 113520tgagttttac tagggcaagc tcaacgttag ggtctaagtc tggtgtacac ttcccctcct 113580tcaggtcgac agtatctctc tccatgatgg gctgtcaggg attgagggag gtgtgatgca 113640agtaatgtaa aactgtcctt cttacccttt tcaatgtatc ttttcttatt tgtgtggcac 113700attcaggtaa tctctcatct gatttcttag ctcttatatt tttgtgtgtg gatagttgtt 113760caaattgatg gttccacaat gagatgagtg ctgcaaaatg ccagtctgcc atcttgctgt 113820tatcgagacc tcaggctttt cttaaaacaa ctcaacactt tggtcgtgca tcccaacctt 113880tccaatcatg gccatcatca acagcatatg aattcaaata ctccaatgtt aacatcttat 113940gcacatgcca ctagtgattc tgtttcatca gctctgctaa tatccaaccc taaaatacca 114000tgcataatct tctggctatt ggagaaaagt cttgaaattt caagtgtgat aaggtcttta 114060atcttaacta gccactcagt ggaatgagat tctcctaatc tgatatggtt tctggtttca 114120ggtagccagc caagattctc aataactttt gatcaacttc ttcacccatt cccctcagta 114180gcttcctaag ttttcagtct tctcaaaact tctaaacaaa ccctttattc cacaaaatat 114240tattaaaaaa tatttcataa agtttcgtgg tcactaacct acagtaagat actaccttac 114300acccactaga atgactacta ttaaagtaca cacacacaca cacaaatatg tgatgggaag 114360gatgtcgaga aactggaccc cttgtacact agtggtagaa atgcaaagct acaattgctc 114420tggaaaagag tatggcagtt tcttaaaatt ttaaaaataa aattaccata tgatccaaca 114480attccacttc tgggtatgta tgcaaaacaa ttcaaagcag ggtctcaaaa agatatttgt 114540acactcctgt tcatagcaac attatctgta atagctaaaa catggaagaa acctgagtgc 114600tcatcagcag gtaaacggat aagcaaaatg tgatatatac atacaataaa acattattca 114660gcctgaaaaa ggaagcaaat

tttgaaacac gctaaacata gatgaaacgt aaggacactg 114720cgaagtaaaa taagtcaatc ataaaagggc aaaaactgta tgatttgcac aaaaactgta 114780tgatggtact tagagcagtc aaaattagac acaaagtaga ataatggttg tcagaggctg 114840tgaagataag gaaatgagga attattgttt aaagggtaca gagtttcagt tctgcaaaat 114900agagagttct gacggtgact gtgcaacaat attaatatac ttaataccac tgagccaaat 114960acttaaaaat ggtgaaatgg aaaattttac attatgtgta ttttacaaca attttttaaa 115020acttaaatga caagtcacat ggctgagtca cataaatttg agaaatacat tttatagtca 115080tttcttgcat attcacaaca tacaccagca tttaaatagg gttacaatat tttcagtaag 115140aatatcttga tttttccaac ctcacccaca attacaatta atatcacaca tccaccttaa 115200cttccttctt ctaatttcat tagatgggca tctttcctct tattcaaggt caatcactat 115260atctgcattt ttctttcttt tatattacct gtatgagaga tacacctgta cttttcattc 115320caatccttac taccttgaaa aactttgctt tgataattac tacctctatt atctgaaaca 115380tctttagtct ttacaataaa ttcactagtc ttacttactt catgttctaa atatttttta 115440aatccagaaa aatttatctt gaattcctat tataccactg tcattgttaa ggttctcact 115500gactgaacta ccttaccacc acacgtttct cttcaatcct ttctctgctg caaggttatt 115560aaaaatacac gtttgacaaa cttactacta tctacctact gagcaatttc tttgctgact 115620cccaaatacc tttctatcac atcttatctt cttcattttc ctacctctca gctagcttat 115680gatctacaag tcctcaactg cctattgccc ctactgccta tgctgcttct gaactttggc 115740tcatacttct gcctagaata agctggtcat gcctctacct gcctgattcc cagtcacatt 115800tcaagactga actaagcatt tcctggccac tcttacttcc ctgccatcta tactggattc 115860ctactgcaag cctgggtact cactccagta ctcctttacg ttactattgt attctggata 115920ccattccacc tcaacgactg aaattatttg cttctgcatc tttcactctg agtataatat 115980aaatttctgt agggtgaaaa ctaattattg tgcatctctg tatctataac gtagtactga 116040tgccttaaac atggtagccg ttgagattgt taaacttaac tctttcctca attcccctat 116100ttccttacaa ctgctaccag ggaagcaaaa agactaagaa ttcaggcgtg tgcaattatc 116160tatctagtag tagaaatcca gtaaactgtt accaaggttt agagttatct ggtaggaaga 116220aaagtaggta tgttcctatg tcccaattta ctgctaaagg attcctttag tttaaccaga 116280aattatttcc aaataatgtg acataattta aagcagtcta gggcaactca aaataagata 116340tcacagtctc aaccaagtta cacttacatt cggctagctt caatgtcgtc agactgaggt 116400tctcctgatc tgtaaaatgc aattgagaaa cagttagcaa aatattccat actccaaaaa 116460aaggtagaca tattacttag gaagacaaat ttgtaaacaa gatttcagca ctaaataaaa 116520cagttttaaa aatacagacg taaacatgaa agaaagtggt cagatcaagt ggccatacaa 116580cacatttatt ttaatggttg gactatcaaa aaactcaatg catgtttttt aaaggaaact 116640gtaaacgcta tacatagata ccaacagaca agccattata agccataatt attcacaaag 116700tcaaatacat gtaataaaac acacaactat aaaacttcct atagccaagt attggttatc 116760cacattaaca aaggtactca ctggtattca cttgcagtat taacctttag attagtgggt 116820cagtaaacta ctccctaggc agaccgacta aagcctaggc cagccatgta gttttgtaaa 116880cgaagtttta ttggcatgca gccatactca ttcatttagg tatcatctat ggctgctttc 116940acgctaaaag gacagagctg agaactcgtg gcagaaacca catggcccac aaaacctaaa 117000atatttattt tctggccctt aagaacaaat gtgccaaccg ctaccgctac tctagacaat 117060gcaaactaaa atccagtacc ctggtaagtt taaagacact taaattagat acaacatcca 117120aatgatgatt atcttggttc aaaaatgaaa ttgtttccct aaaaatacac ttactgtatg 117180tattacctgt caaaagtgta ttacctgagg taaagttttg cactggatag ctgtgaatta 117240acttccgttt taaatagttg ccagttactt tgttatttct aaatatacca ctgctctctt 117300atgtagaaat atttaaaatt ctaagtcact aaaaagcctt tcattttcat ttcacgagac 117360atgaatcagt atttccctat ctctaccatg tcgttaaaaa taagttcctg ttaccctatt 117420cacttttata aacagcatca gcaacaatga atgatgtaag gcgagtcaat ataatacgga 117480tttctaacac ttagaaattc ccaaactaga taaaatatgt tttttgaggt caaaagctaa 117540attttctaat tggcattaca aagtcctgat ttgtgaactt ctcactgact ttaggcttcc 117600aactttaagc aactctaaca agaatcaaaa tcagagtctc acacacacac tctctctctc 117660tctctcacac acacacacac acctgtttga taaatgtctt agcttaaaaa caaaaaaaca 117720aaaaaaaact tcacttcttt atcagactct caaaattacc ctcactgcaa ttcattcaga 117780ttccaaggac aagaatggaa aagaaaaaaa aaaagactcc agcagctcaa atatttattt 117840tagatcttct ttgcaatgta tttaaatttt caattttgca caaaattacg taagaactaa 117900aaactggggc atgctggagg tggcagtgac ttggcagtga cacaaatgca aaaatttcac 117960tccaccctct cccccaggga gcaagcaaac cacctatgtg acacacctgg tctccaccag 118020cctgtgggta agtaccccaa gattgcttcc aaacttgtat ctcccagaca gaagagctaa 118080aaattgatga taaccccatg taccagctga ctcatttttg aaagtgcgac cctccagcat 118140cagatgtcat actgtaagga ggcagagctg gcttgcaaaa caaaaaataa acctgctggc 118200actgagggaa gagaggcaga ttagaagctt tcaaacacgt aggcagcaaa actttcaaaa 118260ttaacaggga aagagaagag aggggaaaaa atgaggacat gatgatagga aaataaaaat 118320attactgcaa aaaggtaaga cacaagaagg aaaacaaatc taagtcttag aagtacaata 118380tgtaactgtt acaaagtttt gagatcctgc aatgggatag aatttaaata cattatcaaa 118440tgataactat atctgtcttc ccaatttctg tttcctaagc aagagaatat gtgaactttg 118500gcatcaaaat gtcaccaaac tgtaacatat taattactac tttttacata tattaaagta 118560ggctattaaa aaccactgat aagacaaact aagtaaacca attcagtata gaagaaggac 118620aaggactgaa agattattac cttaaaataa atctgtcctg gatctattaa acatagcaaa 118680gttgttctat tcaaattgca aattgttttt taatcaagag aaaaatgaat atatacagaa 118740ctagcagaca tttttcttgc agtgaatatt aataatttat gtctgaataa atttctactc 118800atcctaataa cagtatattt ctagaatagt ctaaataagt taccaaaaaa atcccaagtt 118860tagaattcaa actctcaaaa ctatgaaata cgccaggtgt ggtggttcat gtctgtaatc 118920tcagcacttt gggaggtcaa ggcataagga tcacttgagc tcaggagttc aagattagcc 118980tgggcaacat gatgaaacct cgtctcttag ccgggtgtgg tggtgggcgc ctttagtccc 119040agctacttgg aaggctgagg tgggagggtc acttgagccc aggaggtcaa agctgcagtg 119100agctgtgatc gtgccactgc actccagcat gggcaacgga gtgagacctt gtcttggaaa 119160caaacaaaca taaactatga aatatatacc acctagtaat agttcccaag cttgactgtg 119220gctcctaaag agtttactta ttcttttact gccaagttgc tggaaataag aatcctgtat 119280gtgcttgtaa atgcagaaca tataatctaa ttcaaaacat ataaccaaag ggaaattttt 119340gtattaaact attgacttag gttatcctaa agtgtgtcag aataacaagt cagaaaagaa 119400attttaaaac tgaaacaata accaagtaac attagataat aaaaagtaaa tcttagaaga 119460gtctcaattt taggtcttga tttgaatcgc agaaaatatg atcacaaaac gcccactaat 119520ctgaatactg cagcatgagc tagcaaattc aaatggtggc tcacttccaa taacactggt 119580gcagcttctc catcctgcaa gccactcctt ttacctccac tgtaactctc taggagagaa 119640aagtatacta gttttattaa tgtcataaaa acttctagag aaatgtttag ttaaaacgtt 119700caccacataa aaggcatact ttaaaataca tattaagaaa tttttcatgt tttctataag 119760gtttatagta aaatgtttaa aactttgaag gtttttgaga aatactttcc tgttcataca 119820gacaggtctt tgatagtggc tactgatatt tttaatagaa aaaagacact aaaatatttt 119880aaggagatta aatgaagcag gtcaactctc aggcccacta ttcttagcaa tattcctgcc 119940aactatacat tgtcttcatt tctacagtat gtatttcact ttacaatgac agccttttaa 120000aggctgtaaa ataattcaaa attacctttt acaagctgtg gccattcggt gacatcaggg 120060tgatcacagc tttgagtcac tgattaaaaa caggttgtca caccagtcta gttgctacct 120120tgatctgagc gtaggcttaa taactctaat aaattaaaca aacctttggt tagaacactt 120180taatatataa acccaattca ttgttcaaaa aataattttc aaagcttcaa attaggtgtc 120240aatgtaaaat tcatcagaag agtaattatt atagaaaagt ttttaaggac tattttccag 120300cattttgttt ttcttaaatg atctcttcat ttgagaaaat gtaataattc cttgtacttt 120360tatgtaattt tacactttat gaagcatttt ggtcaataat accttgagtc ttgtaacttt 120420tgctttacct gatcgagaca gataatatag taagaatttt ttaaaacaaa caaaaaaaaa 120480ttaagcagcc tccgagtgat taactgactt gcccaagatt ttggaactag tgttgaagga 120540atgattaaaa cccagatata ctgacttcct atcaagggat ggattttttc gtttgtttgt 120600tttggcttag ttttgctata ctgggtttca agatttaagt atacgtaatg agtaaagatt 120660cacaacatag attgagatct ttcaaatatg gcaagtttaa gtggggacta atttactcaa 120720aagattctta agctgataga gaaatcagtc accaagtagt caaatgctta atggaatagt 120780tatatatata cacataaaca cacacacaca taaacacaaa gtctagtcct actaactcta 120840tatttacgta gagtcattta acatcagtca aaaccccttc agtactgcat gaaaatatgt 120900tctactcttt cttggccata aacctactaa gatttaattt catgaaaaag tatggcaaaa 120960gatgaaagca tatcctcctc cctcaaccaa gtatcttttt aaccccttac atggccttag 121020ctgaactttt ctgattgtca cagttcaaat ttagcaggcc tctttactca cactgtagtt 121080ctggcaaaga taccacacat ctgaaatatt ccagtatcaa atgatcattt tgtacaacta 121140gttctttccc ctcacgattc ccctatccag gccaaatctg aggtgtcaac tacagaaagg 121200tgaggaaatt aggaggaaga aacatttcgt agttaacatc ctcaaagcaa caacagtcaa 121260gcgcagaagg tggttaccag tgtggattca gaacccaaca taaaacagca aggtatctgc 121320aaataccaag ggttaaataa aacatgaatg tctgagccca tacctatgca gttatgaatg 121380cagggcttca aaatccagat taggtgagga ctggcctgaa tatacaaaaa taattgtagg 121440ggattccaat aaatggaatt tacagtatct gtaagaaata acacaatctc ccagatattc 121500ctatcctaag catacatttc ctaataagaa cctagacatt gctgaggtca tatgggaaac 121560accaccttcc ctaaaataaa caatgtggtc atgatacagc tttcccttgt gttctcaaga 121620ggctaatggt atgtggaatt agaagattca aacaagtcta actttctatc ttgtaaacac 121680aaaaataata aagttaaaat accttttaat tgactaattg atctcaatgt aatgaaaaga 121740gctatatgta ttttgaaaac tactgagtca agtcctctct tccagtcccc tcttttatga 121800gataccgtgc tcaaaaggct ccaaggccag gctggattta cgtctgtttg gtcactcact 121860agctgagaaa tcttgggcaa gttacttaat ctctccgagt ctcagtttcc ttagacgtag 121920aattagggta atagtaccta ttccagagag ctgtcatgga tgcacaagct aacacctaaa 121980tatctcagaa cagtgcctga catataatgt gctcacaaat ggtcgggtat ggtggctaac 122040acctgtaatc ccagcacttt gggaggccga ggcgggtgaa tcacttgagg ccaggagttc 122100gagaccagcc tggccaacac ggtgaaaccc cattcctatt aaaaatacaa aaattagtca 122160ggtgtggtgg cacgcacctg taatcccaac tacccggcag gccgaggcat gagaatcact 122220tgagcccggg agatggaggt tgtagtgagc cgagaaggtg ccactgcact ccagcctggg 122280caacagagca agactctgtc tcaaaaacaa cagcagcagc aacaacaaca acaaacggac 122340aagctattaa tactcatagc ctaaaatcca aacctgccct ctcaatcttt ttatgcgtat 122400tattttacct tcatagcaat tgtaaatgtt ttcagcaccc tacctgggtc cctcatacag 122460tcttggaagt gtagttagtg agcacctgac acaatgcttt aaaaatggca ggggcccaaa 122520tttgctgaac atatctgtca ttattatgac attaagatac acatgcaata cgaattccaa 122580aaaataaaac aaaaacatac catatttcgc caaacttctg aggagctggt gaattctaaa 122640atcaggctct tacagatttt taacctaaaa tataaaacac ctcactcaat tctttttata 122700taaacaactg gataaacttt caaaaaaaaa aaaaagaggt actaaatttc atcatgaaag 122760caaatttatt ttctataata attatgaaca tccaaaaacg tatcaagaaa caaattactc 122820tatagaggct gccaggatga ggaaagcata ggtaggcagc cctgtggcct gagtgctttc 122880agcaggcaaa gccgtatttc ccatattcat tcacaaagct tactgccttc tatttcttta 122940ctctatcctc cctccaaaag ggctaaatac ataggaaaac tatttgacta tatcccccta 123000atcccttcaa atacctatag caattttgtt tttaattcta tatgctaagc atcggctatg 123060ttaggtacaa atctgggcat ctacctacca ctttccagag tagcagcctt gggtcgaccc 123120tctgaccctt gagtctccaa tgtcaggtta tagtagtaga gaagatgcat taaaaaagca 123180tagtataatg cctacctggc acaaaacagg acttcaatat actacgataa ttacaattta 123240ttaaatcaac cacttaatat atatcaaaac ttaactctta aaaaaaaaaa aaaaaaaaaa 123300atcctgacgc ttacagcaag cccctaaagt atcgctgtaa attcttaact tgtttgtata 123360caatcagaac gttcctcaat ggaacagaaa taaagtcctt taatgtactg cattacatat 123420aaagaaacca aatctcaaag attaagaaac ctatccagtg tcctagaggt tgattgcaga 123480aaatgaatac actgcatatc aacattttga aataaaatta accaactttt aaggttttag 123540agactgagtt tcaaacttca gcctataaca aaattacctg gggaaatcta aaaatgcaac 123600tactcgggct ctacccgcaa agattctcat tcaatgcatg aagtaggggt tcatgaatta 123660ttttttttta tttttttcag acccatccaa gaacacagaa atgaatttgc attttaacat 123720atacttcaca tgacacacag atctcatttg aagaagccct cccttccaaa ttacgagtta 123780ggaacacata atcatagagg aaatacacca aatctataaa aatacagaat aacaaaaact 123840gtaagcatta caaatattag gcacactcac ttgccaattt aaccataaga atcctaatac 123900cagacatagt tctcttactt tatctcttca acagagggct cagtctaata attttataaa 123960taagtatagt ctaataagac atacctatgt tgatctacat ctgtctccct aaaattttca 124020ttttattcat ttctatttct ccttcacctc cttctaaagc tgtttcaaaa cttaatttca 124080caaactgttt ttttttctat cactttttat cataacatcc aacacttctg ttctcctttg 124140caccatgttt atggtgtctt atatttatgg ataatgctac ttattaaaac tgtctacgtt 124200tcatccgttt acacatttca caactaaagg actactccaa caattttcct aagtcttcct 124260gctttaggct ctcctcattt cagtccattc tgcagttaag acttaaaaag ttttaaatac 124320tgctttcaaa taatacttgc aacataattc cctcaactcc attagctcct cagagtacca 124380tttctttatc ctctttcctt tcactgccaa aaagacgtga aaaccactag ctcagctgct 124440gttttcctac ctactgaaat ttttcttaca aaagtttcca tcatttctgt atattctccc 124500agtgctttgg ccccttaatt ctacttaatt tttgtagctt ttaagactgc taatgactcc 124560tcatctcctc tggcttccct catgtaggat ccactcattt ccttccatcc ctctaaaggc 124620aacaaggtcg tctgctagtt catcttccta cctctcatac atggcctctc tccaagtttc 124680ggtcaatagc catttttctt aacctaaacc aattttctgc gcaatgtcat ccattcccat 124740agcacctcta tgcttacgaa cccctaactc tgtatcttta agcccttatc tctggcatgg 124800tatgcctgta actacatatt accaacggta cacatccata tggatgtcta attagcatct 124860acattcaacg tatctaaaat caaactcaaa ttattttccc aaagaactca ctctgcctaa 124920tctcactgtc ctagttatga attgaaaaat ttggccgggc gcggtggctc acgcctgtaa 124980tcccagcact ttgggaggcc gaggcgggcg gatcacaagg tcaggagatc gagaccacgg 125040tgaaaccccg tctctactaa aaatacaaaa aattagccgg gcgcggttgt gggcgcctgt 125100agtcccagct actcgggagg ctgaggcagg agaatggcgt gaacccggga ggcggagctt 125160gcagtgagcc gagatcgcgc cactgcactc cagcctgggc gacagagcga gactccgtct 125220caaaaaaaaa aaaaaaaaaa aaagaaaaat ttggtgccat tcattacttt ctccccctca 125280tttttatttg ccctgaccac tgtgtaggtt gatactcatt cagccttcaa attcttcata 125340atcattaatt cgaaaattgt ctactagatt ttgagtgcct actaggtgga tgaagatgca 125400gtggtagaaa aatgagacat aatttcttgt cctcatgtgg tgaaggaaat gggcacaaat 125460aaaaaccaca atgaattatg taattgcaat gcacagttaa gggttgtgat ggagaaatac 125520aaggggtaag ttgtgtatta taataaaaag tccttagtct gaagaaaagg aaaaccattc 125580tttatgaggg acaagcaaca tttataagac acactaagaa aaatcctgtg aggtaatgat 125640ggtagcacta tcagtccagc tgctcaaatt ggaagattct cagtcatatt ctaaagagtc 125700tctctctttc ccagtatatc caattgttta ccaattacta ctaattcacc cttagaaatt 125760cccctacatt tctacattcc cacactattc cccggtcttt tttgatggct tttagttgct 125820tccagaataa agccaaagct cccaatcatg tcagatgaga ctctacacaa tttggtcaaa 125880acctgccagt gcagcctctt cttccagact agaccagact tcaagagaat atgtgtatca 125940tttgctttta tacttccagt gcttgtaaat gttcatagaa taagcatctt acaaaagaca 126000aattttttct ctttggggac ttgttttcag tcctttaaat gcaatgcagg ttaagattta 126060aactctaatt tgtctaattc taaagaccac gttcatcatt cccttgtacc tgccttctaa 126120tttatactca aaccctatac atttccttaa aaatatatct tactcctttt ttccccaaac 126180ttaccaatac tgtcctactg gtttcccttt tgcctttatc ctcaattttc ccactagcat 126240tatcttccta aaaactcaaa gttatacata tcgttctata ttcaaaagct ttaacacttt 126300ctcatgccaa ccgaataaat ttctaactcc ttcacatttt atatgaggcc cctgcctgac 126360cccagccatc ccagcactac tgcccactac atgacatcat caccaggtac cagatctagc 126420cccatcattt gaccactggc tataatacac tggatatctg tgtttctgac ttcaatgtgt 126480tcgcttctga ggagacttca ctcagaaatg gttctctatc cattactaac ctctatcccc 126540cacccaccta tcatgccatg ttaaaaactg ccctcattct aaacactgta tctcacaggg 126600ttgtctaaac tcttatacat aatcaccgtc tccttcatgt cttattatac attcttaata 126660ccataaaata attaatgtct ccttatattt attatgttat accttacatt acagctatct 126720gtatacattt gtcagtgcct ctagttgtaa gctgattgat gtagggatca tctcttccat 126780attttaattt tcataataat ggtgactatg ttttcttgac accatacctc ccctctacaa 126840ggaaaaaaaa aaaaaaagga atcaggaccc atggcctttg agctagttcc aaacgtgact 126900catagatgaa caaaggtatc atttcttccc aaagcaacac aaatataagt aaatgccaat 126960ttaagaaaca ttttatttca aggactaaat atgtaagaat aaccaaaaca actgtttcta 127020ataatgtccc tttctctacc agacattaaa acatattaac ttaaatggta taattaaaat 127080tagcacaaca ccttcaaaaa tggagtatac actaaatata tgcatacccc ctgacttaaa 127140aatttccctc ttaagtatac atcaaaaaaa ttaagtacac atattcacga gaagacaatc 127200actgaaatgt ttagagcagc agcaagattc ttaacaattc caaaccaaaa atgattaaat 127260gtccatcaac agcagattaa actgtggcat attcttaaaa tggaatacta tacagcgact 127320atcataaaca attactacaa ccaacagtgt ggataaatct cacaaacaca ttaaaagcaa 127380tcagacacaa gtaagtgtgc ttgattctag ttaaaggacg aaaacaggca aatctaatct 127440atgctatgag aagtcaggac agcggttaca catggaagaa aacggagggt aacagagcac 127500aagcgagcac acaggagcga gcacacagga gcgagcacac aggagctgcc gatgttgctt 127560cttgatctga acgctagcta aatacgtgtg ctcagtgtgc gaaagtgcag caagctatac 127620atttggaagt gcactcttct gtatatttaa aagattttta aaaaaaatct gatatggtat 127680tagcattaga caaagaggta aacacaacag aaagctaatt tcagagaaag atccaaaagt 127740atctgacagt tgctccttct caaacaagaa ttttcagacc ttggataaca ggcagcacag 127800gactgtaata catgaaagga caaaaacaaa ggaggtgagc catccactgc ttggatgcct 127860gcctggaggt aatttccaac ctgcagtcaa tggaggggaa cccaacgaga agacagcagt 127920ttcagtgagg tcagaatcaa gagatcagag ctgaggaagc cgaagtgggt aaaatgtatg 127980acaagtgcca gacaagaggg gtgcttgtct gagaaggcag agaaatggct tcagaaatct 128040gtcatgtgag agtctactgc tgagtactaa ctgtacacga ttaaggtaag tggaagctag 128100ggaaagaggg gcagtgagct gaaatattcc cagtgcttat aaaggaccgg gagactccta 128160aaattccctc taacctgagc gaagagaaca cggtgacact gggagccttc tctagacaaa 128220ctccaatgta gccctgaagg aataaaactg accagcaagt tatttagctg gctacaaaac 128280aaaacactct aaaagaaaac aaaatctagt cacccaacaa caaaatatta caatgtccag 128340catccaatct tataaaatca ttaaatatgt aaaaataaaa ataaaaataa actaggaaaa 128400tacaacccat aatcaagaga aaaaaccaac aaattcagag tgaaaaaaat catgaaatca 128460ataggtaacc tgattttcag ttattacaaa tatgctcaag atttcaaaaa aatacatgaa 128520gatgaaaatg aatcaaaatg gaatttcttg aaatataaag caaaatatct gaaattattt 128580aacaaggcag gagaaaaaac agcaaaatag aagacaaagc aaactgaagc acaggcaaat 128640aaaaaaactg aaaaataatg aatatgacgt taatgacctg tggataatac caaacagtct 128700aacacatgtg taagtggagt cccagaaaac gaggaaatga tgaaagagga gaggtggtaa 128760aaaatacata tatatatata tatatatata tatatatata tatagaagaa acaatggcaa 128820caatcttcca aatttaagga aaaccataaa tatacagatt taaaaaaaac acaactctta 128880gcatggtcat catcagaaac aaaaagggac aagaaaaaat cttaagagca gccagggaaa 128940gaagacttag tatcaggaag aaaagataat tagcattatg catcacttaa cagcacggat 129000acattctgag aaatgtatca ttaggtggca tcattaggtg attctgtcat tgtgcaaaca 129060tcacagaatg tacttacaca aatctcgatt agatggtata gcttactaca gaactaggat 129120atatggtata gcctgggtgc tcctaggtta taaacctgta cagcatgtta ctgtactgat 129180actgtaagca actataacac agtgtaagta tgtatctaaa cctagaaaag gtacagtgaa 129240aatatggtgg tataatctta aatgactatt gtcaactatg atctcttgtt gactgaaatg 129300tcattatgtg gcacatgact gtataatcag acttctcatc aaaaacaaac gctgaaagaa 129360aaacacatca actatttaac caagagaaaa gaaacatata tgtccacaca gagactgttt 129420tcacacaaat gttcattgta cctttattta taatagctag gaactgaaaa cagcccaggt 129480gtcccttaat agatgaagtc acaaaccact gaaacattca acagtatgga tgaatctcaa 129540aaacattatg ctgagcaaaa agaaactttt tacaacacat aagtattgta tgactctgct 129600tacataaagt tctaagcaag ttaaactaag atgaaacaat ctgaacaatg ttaggttcta 129660cgggtggtgt ggggggaagg aataactgga aagaggtgca ggttctgtac cctgatagaa 129720ctttctggca aaggtgtatg

catttgtcac agctcatgaa atggtgtatg tggtcaactg 129780tattttccaa agatggtcac aacagaagcc attctacatg ctcttaacaa tgtaacactg 129840ttatcttttc atcaggtggt ggagtctatg gcccttctct ttgaatctga gcgaaattag 129900tgactctttc aatccaaaga ctacagtcgt acctcaacta tctacagagg actggttcca 129960ggactcccat ggatcccaaa atccaagaat gcttcgaatc ccttatataa aatggcatag 130020tatttgcata tgacctacat gcatcttcaa atatgttaaa ccatctctag attacttata 130080ataccaaata caatgtaaat gccatgtaaa cagttgttac aatgtattgc caaagacaag 130140ctctccttgc caatccctga ccaaatgaga gattcatgag caaaataaat agttgctgat 130200atgtaaggca ctaagtttgg ggcaatgtat tacacagcaa taaataactg gaacagcata 130260gtaaattgta gttaaaaaca ctaaactact aagttttagt taatatacat gctgaagtta 130320agggtgtggt atactaacat ctgcaactta ctttgaaaag cataaaaaaa gacagttgaa 130380gacaggatgg atagatggac aacatagaag acaaaaacaa atataacaaa atgttagttg 130440tgaaattata ttctacaatt attttaaagt ctctgtggct tgaaaaattg tacaataaaa 130500tgttgaagag tgaaataaag acatttccat acaaataaaa gctgaaagat tttgtagcca 130560gtagacctac actttagaaa tgtttaagaa attttttcaa gtagaaggaa aataacacca 130620agaaacttgg atctacaaaa agaaatctcc acaaaggaaa agaaataaaa atggtaaata 130680agagggtaaa tagaaaggaa tgttattctt agtttttaag gttctttgta agacaactgg 130740cttttgaaag aaatagtttc aaatgctact gtggaatcta taacacatat agaagtaaaa 130800tgttaagctc acaataccaa aacgaatgag ggagagtaaa tggatgtata ctattctaaa 130860gttcttacat tatacattaa gaggaataat atttctgaag gcagactgcg gtaagttaaa 130920ggtgcatatt gttaatccta gaagtgtcac tgggggaaaa aacagaggga caagctaata 130980atggagacaa aacaaaattc caaaaatgct ccattaaacg aaaagaaggc agaaaaggaa 131040gaaaagatga gacaaataga aaacaaacag tgtcttaaac acaatacagc caactgcaat 131100aaatgtaaac ggacttaaac aaattaaaaa gcaaagatcg taaaaaatca atctgtaagc 131160aagatgcaac tatacgctat ctaaaagaaa gacattaaat gaccaataag ttgaaaataa 131220aaacacagca aaatgataca atgtgcaaaa attaatcata agaaaggaat ggatacacta 131280ataccaaagt agactttaga tagggaacat taccggcgat aaacagagac atttcataat 131340gataaagaca tcaattaatc aaggagacct aacagtccta aatgtgaaca cacctaatga 131400cacagctttc aagattttgc ttcaaagcat atgaagcaaa aatgaacaga acagaaggaa 131460gataaactca aaattacagt tgaagatttc aacattcctc tcttgataat tagtaagtgg 131520acagaaaatt agtaaagata aagaagaaat acatgtatct atctgacaaa gaacacacgt 131580tctgaaaaga atgtgaaaat atttgaacag acacacaaag aagaaaactt ctcaatggca 131640gtcatgaaga aaatgtaaat taaaaccatg actttttaca cccattagaa tggcaattat 131700tttaaataaa aaaacagaaa ctaccggcca ggtggtggct catgcctgta atcccagcac 131760tttgggaggc cgaggcgggt gcatttgagg tcaggagatc gagaccatcc tggctaacat 131820ggtgaaacct cgtctctatt acaaaaatac aaaaaaatta gccaggcatg gcagtgggcg 131880cctgtagtcc cagctacttg ggaggctgag gcaggagaat ggcatgaagc caggaggcgg 131940agcttgcagt gagctgagat cgcgctgctg cactccagcc tggcagacac agagaggcta 132000cgtctcaaaa aaaaaaaaaa aaaaaaaaag gcagaaacta ccaagtgtta gaaaagaggt 132060agaggatgta gagaaactgg aacccttatt cactgctggt gggaatgtaa aatggtgcag 132120tcactgtgga aagcagtacg gcagtctcta aaaaaactac acatacaatt accatatggc 132180ccagcaattc cactcctaag tacataacca aaagaattaa aagaagggac tcaaacaggt 132240atttatatac caatgttcat agcagcatca ttcgcatagt taaaaggtac aaacagccca 132300aatgtccact gacaaataaa tttaaaaaaa aaaaaaaagt gatatatgca tacaatgaaa 132360tattgctcaa ccttaaagaa tcaaatttgg atacatgcta caatatgaat gaactttcag 132420gactgatatc gtttgaatat ttgtcctgta cagatctcat gctaaaaata caatccccaa 132480tgttgggggt ggggtctggt gggaggcaac tgggtcacag gcgcaacccc tcatagcttg 132540gtgctgttct tgggatagag agtgagttct catgagatgt ggttgttcaa aaagggtgtg 132600ccacttcccc accctctctc tcttgctcct gctctcgcca tatgacatgg ctgctcctcc 132660tctgccttcc accatgagtg aacgcaccat gagggctccc cagaagctga gcagatgatg 132720gtgccatgct tgtacagcct gcagcaccag gagccaaata aacttctttt ctttttaaat 132780tacccagtct cagatattcc tttaaggcaa cacaagaatg gcctaacaca aggacattat 132840tactatgaaa tgaaatgtgc cagacacaaa aggacaaaaa tcgtatgatt ctacttatat 132900gaagtacctg gaggagtcaa attcacataa aaagtagaat agtgattaca ggtggatggc 132960gagagtaggc aatgaagagt tactgtttaa tggatagagt ttcagactga gaggatgaaa 133020aagtttcaga gatggacagt ggtggtggtt gcacaataat gtgaatgtac tttatgtcac 133080tgaactatac acttaaaaac agttaaaatg gtaaatttgg tatgtgtatt ttactaccat 133140taaaaaaaac caaaacactg caatgggata ccactacaaa cctctcagaa tgtgtgcaat 133200taaagactga gaacatcaaa tgctgacatt tggagaattg gaactctcat acactgctga 133260tgggaatgta aagtagtata atcatccgga aaatagtttg gcagttcctt atacatttaa 133320acataaactt cccatgtgac ccagtaattc caatttaagg tatttaccct agaaaaatga 133380aaacatatgt tcacacaaag acatgcacac aaaacaatac ctttattcac aacagcctaa 133440actgaaaaca atctaaatgt tcaccagaat atgaatgaac tgtggtacac tcatattgta 133500tttaaaaata caaaacaaac aaacaaaaaa tctgtgataa acacaaattg gatgaatctc 133560aaaaacatgc caagtgaaag aaaccagaca tggaagagta catgttactt gattccgtgg 133620atatgcaatt ctagagcagg caaaactaat aaacagatca gtggttgcct ggggccaaga 133680gagagaaatg actacagaca ggaaggaact ttttggaggt aattcaattg ttctaaagct 133740tgactgtagt agcagtgata gtgtatatat ttatcaaaat caataaaact gtacagttaa 133800aatgggtgca tttcattgtg tatcaattat acctcaataa acttgatgtt tttcaaagtt 133860tatgagaacc taatacagaa gaaggtggca tcttcagtgg agacacgatg ccttacaaga 133920cagtcagcta cccgagtgga agaaaataaa aggtagacat aattcccaaa ccatgtgaca 133980aatgcaatcc ggatctaaac catatcatac catatccaaa attatgctga atgttcagat 134040ctaactttat aaattaaagt caataaaatc ataaaagtac cagaagaaaa tacaagagat 134100tggccaggta tagtagctca tgcttgtaat cccagcactt tgggaggctg atgtgggagg 134160acagcttgag gccagcagtt caagaccagt ctgggcaaca cagtgagata cgttttctac 134220aaaaaaacaa acaaaccaac caaagaaacc cgactagcta tagtggcaca ggcctgtaat 134280cttacctact ggggaagcta aggcaggagg atcacttgag cccaggagtt tgaggttgca 134340gggagctatg gttgctccag cctgggtgac agagtgacat cctatctcta aaaagttttt 134400gtaatatata tatgagatca tatttatatc atctttgtta gcatgctgaa actcagaagc 134460tataaaagat ggacagatct gcctacataa aaactttaaa atttcacatg actagatact 134520ataaaattaa agataagtaa taagataata tttgcaaaat ataacaaaga attaaatgat 134580aaataaagaa tgcttacaaa tttaaaaaag aaacaacaca atagaaatgg accaaaaaac 134640cagacaattg cccaggctat ttataaagat ttaaacagat tttatatata cataaatcaa 134700aacannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134760nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134820nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134880nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 134940nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135000nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135060nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 135120nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcagctgct 135180ctggtcaaaa aactttggca ttatctttta cttctccctt tatttcccac tttcatctgg 135240tctactacca aatcctatgg actctatttc gacatatatc aagaattcaa ccacttctca 135300ccaccctcac tttgaccact ctgtccgttt cagtcacctc ttaatctgta ctctaccaat 135360tggtctgttt ctgcccttgt cccccttttc agtctactct caacacagca acgagagtga 135420tcttgctaaa cctaagtcat actttttcac tactctgctc aaagacttcc aagagcttcc 135480catctccttc aatggtaaaa atcaaacacc ttattatgat caaaatatct gtaccctcca 135540ttatctgtct ctttatttcc catcatttac ttttctaaag ctacaatggc catacctcga 135600gttcttcaaa cacatcaggc actctcactt tggggtttcc ctattcacta caccctttgg 135660ctgaaatgct cttcccccaa atatccacat ggctagctct ctcatctcct tcagttcttc 135720acccaagtta ccatttcagt gaagttttct ctacctaccc tatctaaaat ttcccattac 135780cctttcacct acaaacactt cccttatttg ttttttctcc ttagcactat gtaacatata 135840ttttacctat ctttatgatc tctcttttta gtattaaagt gccatgaaag caaaggattg 135900agtgggtttt atacactgct gtctccacca gctcctagaa aaaaatgccc actacacagt 135960aggaattcaa gaaatgttta ctaagtcaac cagatggatg tgtatgcagc caaccatttg 136020aaagattctt ataacatagt taagagaaaa aaataagtta catgaaatac aagttttagt 136080acacaaaaat ctgtacacaa ggaattaaca tgaaattttt taaaaataca atttgcaata 136140ccatcaaaac ataaaattct tagaaataaa tttttaaagt aataaaagta gtacaagacc 136200tgtatactga aaactacaaa acatgaaaga aattaaagaa caccttaata aatggaaaga 136260tgtcccatgc tcatgaatga gaagacagta ttaaggtagc aaaacttccc aaatagacct 136320acagattcaa cacaaatcca ggtcctatca aaatcccagt ttcatttctt gcaagctaac 136380actaaaattc catggaaatt tgagaaaccc agagacccaa aacaattttg aaaaagagta 136440agttagaata cccacatttc ctgatttcaa gacttactac aaagctacag taatcaagag 136500tgtgtgttac caaaaaaaca gacatacata tcaatggact atataactca gagtccagaa 136560ataaattatt agattttgtc aattaaattt tcacaatgac gccaagataa ttcagtgggg 136620aaagaatact tttcaacaaa tggtgctggg acaactgaat tgccacttgt aaaagaatac 136680aattggatct ccccctcaca ccttacgtaa aagttaactc agaacagata ctaggcctaa 136740atataagaga tacaactgta aaactcttag acgagaacag gtataaatct ttacgatctt 136800ggattaggca atggtttctt agataccacc accaaaagaa caagcaacaa aagaaaaaaa 136860aaattgactt caccaaaatt agaaactgtg tgcttcaaag gaaaatgaaa agacaatcca 136920taaaatgggg gaaattattt gaaaatcaca tatctgatga gggacttgta cccagaatat 136980atttcaaaaa ctcgtacaag ccaacaataa caaaaattta aaatggcaaa agaaatagtt 137040ctccgaagat ggccaatcag catatgaaat gacgttcatc accgtaagtt gtcaaggaaa 137100tggacatcag agccacaaag atccttcaca cacacaagaa tggctgaaat aaaaaggaca 137160gctgtgttaa aaggaagtag agaaatcaga accctcatac actgctggtg caattgcaaa 137220attagggcag ccactttgga aaagagtttg gcaattcctc aacatgttaa acacggagtt 137280accctaggaa cccagcagtt tcactcctag atatatagcc aagagaactg aatatatatg 137340ttcacataaa atcttgcaga taaacgttct ttgcaacagt attcatgtca caacatgaat 137400ataccttgaa aataccatag taaataaaag aacctgtaca caaaaggaca catagtgtat 137460gattcccatt tatatgaaat gtccagaata aataaattca tagagacaaa gcagattagt 137520tgttgccagg ggccaggaaa gggaagaatg gggaatggct gctaacaggt taggatttct 137580tttggggatg atgaaaatgt tctaaaattc gatagtggtg atggccgcac aactgtgaat 137640atactaaaaa ctttaaaagg gtgaatttta tagtatgtga attatctcaa taaagctatt 137700aatagttttg aaagttagag aatatgtttg gtatgatttt tttttaaaca ccgcctcccc 137760aaaaggaaaa aagctgtatg tatatcagta cctttacagc tatcttacat tttatatttg 137820attggtaatt tccttccaag catttctttc cccctacatt taaacacatt taacaactgt 137880taagttttag aactggctat gaagggaaga gaaacatttc atttcttctt atacttaaaa 137940aaaaatagct ttttattttt cttttctata tttcctcatc ctcaaaaata gattctacac 138000accaatattt gagggatggt ggggcaacta ccacaaaata caagaggctg tcttttggaa 138060atgtaaaaat gttagaattc ctaggaactt gtcttgattt atgggactaa cctaatattt 138120aaaactgagc ccatgctggt aaatctgggc tatacagtca tacatacata gaactgtggt 138180atcacaacac atccactgtg cactggaaga gtactataat ctccttataa tgtgcacctt 138240ggctctcccc tcccttgcgt tctttgacat tttttttttt aaataaggca tgtttatttc 138300ttatttacct actaactaca gaaggtagac tcacgatgtg ccaatattaa aatgttattc 138360cccattaaaa tacatttact taagtgtttt attacatgac ttaccagcaa tggaagaatc 138420tcatctgaga gtagttctca actgtcatct atgaaaatct ttagaaatac caaatcatca 138480gaattacttt aatttcctgg cttttaatca taaaacattc ctactggtca acaattctac 138540aatctgaaaa gagacatctg taccatatag tcccaatgat ttattattct gaaattttcc 138600tctgggttat ttttacacta taattagagt tcaaacttcc atagtgccta gcctaaaatt 138660aaacctagaa ttatgccaat ttatgcctgc tatatatgca cacacaaaag acttataatg 138720caacatactg aaagaactaa aggtaggtgg gtttttcttt gaaggaatca tagctacata 138780atttcaagtt taatctgtta catttatctg cctgtaaaca tgcctgtagc atgtaaactc 138840ataaaacgaa aactgattta gtaacaaaaa aaattagctt ctaatttaac ttcaaaatgc 138900ttttcaaaaa atcgacccca tctaattcta agtgaactag accacacttg atagaaaact 138960gttgttctaa aaaccatacg tacttaacat aaatagtaaa catacgagaa catttagtct 139020tctattaatg tcttacaaaa tacttcaatg tggcctagcc tggttataat ttttttcttt 139080ttttttaagg gacagggtct ctgtcaccga ggctggagtg cagtggtgtg ataatagttc 139140actgcagcct ggaactccag ggttcaagca atcccccccc acctcaggct cccgagctgg 139200gctaatttta tttttcatag ggataaggtc tcactttgtt gcccaggctg gtctccaatt 139260cgcggcctca agtgattctc tcacctctgc cttacatttc cttttgagct gttattatca 139320actggagtaa catttctagt tgggggaaaa aggaactgat ccttatcaat ctgtagatga 139380atacttattc agcacttaca aataactgaa tgtgggcatg aaaaaaagaa tgacagatga 139440taaacattta ctgttctcac agaactatca aatacaggaa taagtaaatt acctaatttc 139500agttgagata aatacgactc agaattcaat aaacagtata aggtgaggat gctagaaatg 139560cccacataat gctgacaaac tacacattgg catatccatt taagaaaaga atttaccaat 139620atctagtaac cagcaatttc acttctacgg ttctttccaa aacacctgca catacactca 139680cgtaaaaata aacactgcag cattgcttat aatagcaaaa agaaaacaac tcaaaatatc 139740catcaatagt gggatacatt ttatctatgc ataaaatgaa gtgccagaca acagtgaaac 139800tgaataaact acatctacat acatgaacat atacacctca aaaacttgat actgagttta 139860aaaaaatcaa gctattgaaa gacagtgcta tacatatctg taaaaattta atacaaaaac 139920caccaccaca tatcacttat agatacatgt acacatgtat agtaaaagta taaaacaagg 139980atgtgaaata tacttaagaa tctcaagaca gttgttactt tgtgtagaga ggggtaacag 140040gacttgaaca gttggggttg ttaaaagtca ttatgtaact ggcatccttt cccccaaggg 140100ataaaaatgg acataattta catttatagc tggtggataa atgggcattt gctatgttgt 140160tttctggatg tttttaaaac tttacaatta aaacatatac acttatttta aaaacaaaca 140220aaagacacat tagcatataa tgtccaagct gatatttacc ttggaagatc atctaatata 140280aacctcccta cttcactaaa aaaaatcaga aaagatgtca gaataaaaat atgatacagg 140340ctggacgcag tggctcatgt ctataattcc agcactttag gaggccaagg caggaggatc 140400ccttgagccc aggagtcaag accaggctgg gtaatatagt gaaacttcat ctctacaaac 140460aaaacaaaac aaaacaaaac aaaacaaaac caaaccacca aactttttag taagaatgta 140520atagggttgg tgtggtggct catgccttta atcccagtac tttcagaggt cgaggcgggc 140580agatcacgtg aggttaggag tttgaaacca gcctggccaa catggtgaaa ccctgtaact 140640actaaaaata caaaaattag ccgggcgtgg tggcacacgc ctgtagtccc tgctactcag 140700gaggctgagg caggagaatc gcttgaaccc aggaggcgga ggctgcagtg agatgagatc 140760gcaccactgc actccagcct gggtgacagc gtgagactct atctcaggaa aaaataaaat 140820aaatacgtgt gtgtgtgtat gtgtgtatat atatatgcat tatatatata cacacatata 140880tatatgtata tatatataca cacacacaca tataaaatac atatataata tatatactct 140940tgtagcaggg caattttgga cagatactag gttatctgga acagcacttt ggggaattag 141000atcctgacca tttaagagag aaatctggta ctgactagca atatggacaa tgtgtgcacc 141060accctcactc cccctcccct cctaggaatg ccatgtcaaa tttagcacag gcataggcag 141120taaaactgac ccctagttct gcctatcact atttaaaatt actttcagtt ctgagtgtag 141180aggactgtcc ccacagcaga tatctaaaag caggcctgaa agaactagaa agtagttcat 141240tcctcctaaa actgtacatg aaaaaacaaa attaaaaaat gaactgagag caaacattta 141300tgctctctta taattttcaa tccaggattt aagaaattat ttaacatcag ctgagacgtg 141360accaacctat tctccagttg agatccatct atttttaaat tcactccccc acctttcatt 141420cagcctaaaa gaggaaataa gaatgggaag gtggggagga cttatcccat actgagttaa 141480ataagaccac aagatctttt catttttaca atatttgaaa tacagtacac tgagcaataa 141540atttaaagaa atatacacta tttgaaatgc ataaatgttg ctttatgtgt ataaaagatc 141600aaaattagct cagttcttct aatacactac acttaagcgt tcttcgatac tatcgtcaac 141660ttttcagaaa tattcccatt ctgtgaagca ggatgtattc ctaccttatt caccagactt 141720tatccacgta acttcctatt ctcccactct attctttaaa catgttcaac atgttacaga 141780gatggcaaaa tgtgttaaaa aagggaatga gaatgaagca gcttaagtaa ttaggaaaac 141840tttataattc ttagaaactg aatttggatg atctttaaaa aaaaaaaaaa aagaaaagaa 141900aatctgctat ttgagatgaa gtgtcgcctg cccaggctgg agtgcaatgg cacgatctcg 141960gctcactgca acctctgcct cccaggttca agcgattctt gtgcctcagc ctcccgagta 142020gctgggatta cagatgtgtg ccaccacgcc tagctaattt ttgtattttt aggagagaca 142080gggtttcacc aagatggcca ggctggtctc gaactcccga cctcaggtga tcagcaggcc 142140tcagcctccc aaagtgctga gatcacaggc gtgagccaag gtgcccagcc taaagagcat 142200attttcaatc accaacacac aacatgactg ctgacttcag aaaactagga aagaaaaacg 142260ttacccaatt aatcgttgtg tgaaatagga tttatccatt agtatacata tcacatacaa 142320taggtgcaca aaagattttt tccattccca aaacaaaaac ttaaaatatt taacaaagat 142380aatataaata tccttattta tagcacattg taaggttatc aatattggtg catataacca 142440cagaggcaca ataaatagaa ttctgaggaa aaatgtatta agacaataaa caagctggaa 142500ataaccccta tttcccctta tgacccatta gctttatata gaaaatactg ctgtgcactt 142560catcctttgc tttctaaaga acagcattat ggttcacatc tgttaagaga cagaaaagta 142620tttgttggaa gtatgcaagg aagaaattcc tgtactgtta cgtgcagtgc taaaggaatc 142680atctgagaag aaaataaaga acatgagttt aacatgagct ttagagaggg aacaagagtg 142740caagaaagcg acaaaatgtg acatatgtcc atagtgaata catggtattt tattttcagt 142800atttgtatat ttaatttgga ggtggaaaga ttcagaattt attctgcatc acaaagatta 142860taaaaatata tgagttgggg tgttggctac agtacacaaa ggcaattcta agacaaacta 142920ttaacagaag cttgaaatgg ataagccatc ccttctgcta tgagacagaa gtcattacac 142980gaaaagaatc tgtgtagaag cacagcaatg gtagcatctt cattcagccc acagctgtgt 143040aatttgtgtg caagagtgct tgtcttaaac atgaaaagga acatcctgaa ttgctgaaag 143100tatatacaac gggcacacat ccagaactag aaaagaccta ccaagcctgc atgctatgaa 143160gagataggtc aaaaaatttt tctcatgttt ggtttttata actaaagtct gtgctagact 143220aacctcaatt gatcctgagg ggtacagatg aacttctgga acacaatgaa ctgttaagag 143280tcacagcttt agactctaca ctgtccagcc aacagtttca agaaaaattt tatccttctt 143340gtatgagtgt tcagggatca tatactacct ctatgctctt acttgaagca cagatacaga 143400ccttaataaa taaagtctta catcagactg tcttctataa tcacatgtga ttccttacac 143460gagattcctg ttcagatttc ctgaaaatga actataggaa taatcttatc caatatgagt 143520ctatgtcccc taaaaatctc ttttcctttc aggtcaatct tatgattcac ttgccataga 143580tcagattaac acaacaggtc tgatacagca aattacaaaa gagatttgac ttttgattaa 143640tacactgaat aacgtaccac aaactattaa gatctattgt ccatgtcaaa tggagttcac 143700atattcagaa tgaattcaca cactaaaaat tttccaatac aaagtttcta aaaagttttt 143760tctttttttg agacggagtc tcgctctgtc gccaggcagt gggtatgatc ttggctcacc 143820gtaacctccg cctcccgggt tcaagtgttt cccctgcctc agcctccaga gtagttggga 143880ctacaggcgt gttaattttt gtgtgtgtgt attttagtag agatgggatt tcaccatgtt 143940ggccaggatg gtctcgatct cctgacctcg tgatccgcct gcctcggctt cccaaagtgc 144000tgggatgaca ggcgtgagcc accgcgcttg gcctctaaaa agcctcttaa tctacatttt 144060caagaatgat cacatttgaa aagcatactc ttttaaaaca aagcaactat caccttcatt 144120tgacagaagg aggaacaaac gcttcaaaag ctaaataacc tgctcatgac cattaggaag 144180ctatagttaa aacaacctca agaccaacct ccttcctcca tttcaatgac tctgacacac 144240ttaaaatttt ataaataatt ttagggatta tcagtcataa ggcttagaat caaatagtaa 144300cgactcatgt acaaagacca gtattttttt aaaatgctca gctgggcatg gtagctcaca 144360cctgtaatcc tagcactctg agaggccaag gggcttgagg acaggagttt gaaaccaact 144420tgaacatggc aagaccccat ctccacaaaa aataaaaaaa ttagccaggc atggtggcat 144480atgctaatct cacctactca ggagggtgag atgggaggat cactcaggcc tgagactgca 144540atgagttatg acagcaccac tgcactccaa cttgggcaac agagcgagac cctgtctcta 144600aacaaataaa taaaattaaa caaaaagaaa tgctttggga ggccgagacg ggcggatcac 144660gaggtcagga gatcgagact atcctggcta acacagtgaa accccgtctc tactaaaaaa 144720atacaaaaaa actccccggg tgcggtggtg ggcgcctgta gttccagcta ctcgggaggc 144780tgaggcagga gaatggcgta

aacccaggag gtggagcttg tagtgagctg agatccggcc 144840actgcactct agcccaggct acagagcgag actccgtctc aaaaaaaaaa aaatgctaac 144900tttatttcac tctcagatag gcctaataat taataagctt ctttttgaat tattatccat 144960aatggcattt acctgttaca gataactcac atcaaagcac taagcaacat ttttgagagc 145020aaatgaaaac aaaagctgcc aaagaagctg tgtattaata agtcatttca ttaaaaaaaa 145080aaaaacttgc caggtgttgt ggctcatgcc tgtaatccca acacactggg aagctgaggt 145140aaaagcctag gagttcaaga ccagcctggg caacacagtg agaccctcat ctctataaaa 145200caaaaatttt taatattatc tttttaaaaa ggacttattt atatagcatc tttaagtatt 145260tcaaacattt catgtttgga ttatggggta tgtttctaga attaacacta tttctattta 145320aggtaatttt acatcatttc ctttattaga aaaaataaca gatctgtgga tggcattttg 145380tgaaggctgc cttctattaa tagtgtataa aacagtcatg tatctccaaa acggtgggtg 145440agtaatcaca aagcaagtgt aactattcat cccaaaaggg aggacagaac atgtgccaat 145500gtgtgcagag tcctgaacta gagacttgga ttctagttct ggtcctgaca cctgggtgtc 145560tttcagaaaa tcattttaaa gctttgcact ccaatttcct catgtataaa atgactggag 145620cagctgacct catagacaat cctacttagg tagcatctat caatgtattg ccagaagaca 145680gaatcataat catgcccccc aaaattagag tatttgaaga caaccgccaa agtaaaaatt 145740ttaaattctt ttttattttt tttaattttt attttttttg agactgagtc tcactctgtc 145800tcccaggctg gagtgcagtg gccagatctc agctcactgc aaactccgcc tcccgggttc 145860atgccattct cctgcctcag cctcccaagt agctgggact acaggcgccc gccaccacac 145920ctggctaatt tttgcatttt tagtagagac ggggttcacc gtgnnnnnnn nnnnnnnnnn 145980nnntttttag aaactttgta ttggaaaatt tttagtgtgt gaattcattc tgaatatgtg 146040aactccattt gacatggaca atagatctta atagtttgtg gtacgttatt cagtgtatta 146100atcaaaagtc aaatctcttt tgtaatttgc tgtatcagac ctgttgtgtt aatctgatct 146160atggcaagtg aatcataaga ttgacctgaa aggaaaagag atttttaggg gacttagact 146220ccttacacac acacacacac acacacacac acacacacac acacacacaa atactaatac 146280actacatgcc agatactggg gtggtgggct gggaccaagg catgaagtaa taatgcatgt 146340gccaaagggc tgaacttttt aaaatgagta caaaagtaaa ttttcagagt agcagctggt 146400gggtaggagg tattgagtcc acagaactgc tcttagattg gaagggcatg ggagaatgaa 146460gttccattat gtgatggggt tttacacaca cacacacaca cacacacaca cacacacaca 146520cgcacacaca catgaatgtg agaaagtcac tcaattataa gcattcaaaa agacagacag 146580ctttcttgtc tttgtggcgt cagtacctag ctcagctcat agaagccaca ggaggaacct 146640aatcaatgtt agttgatcat ttactgattt accaataact aattcatgac tgctctagtg 146700ctgtggtctg aatgtgtccc ccaaaattca tgttgaaagc ctaaccgcca aggtgacagt 146760attaacagct ggggcctttg ggaggctttg ccctcattaa tggaattaat gcctctataa 146820aagaggccct atagagcccc ctctactctt ctactacatg aagttatagt gaaaagacag 146880ctagctgtct atgaacaagg gaagagggcc ctcactaaga tgtcaaattt gtcggtacct 146940tgatcttggt tggacttctc agcccccaga accgtgagaa ataaaacttc cgttgtttat 147000acgccaccct gtctgtggtg ttttgtaata gtagccagaa cagactaaga catctagtaa 147060cgcattctct ggtaaacact aggaattcct ggccttaaag agttcatagc taatgagaaa 147120agaaataaac aatataaagg ataacatgct gtagtccatt atatacaaag tgcttccaga 147180ccattcagaa aaactggata ggaagatttc taaggtaata cttattgagt gcctgcgtgt 147240taggcgtttt acatacttta agcctcacaa ctctggggaa gtttcaccaa cccattttac 147300aggtgacaaa actgtggctt accaagatta ataaggtcag aattaaaagt agggtctaat 147360tccaaagaca ttaccactat acataccaat ttgtcttgtt actacatttt atccttacat 147420ttggcagtga ggggaaaact caaaaccagt caaaaaaact tttgtcctga accagcataa 147480aaagagcacc taagacttca tccagtattt tactgaaaga tcacttctaa aagtttgtct 147540tcaccccgat ctaaaaatta gaaaactaat tttctttcag cagctgcata agtacataat 147600gtaatgtttc tacatttaga cctatacaat tataggtcta aacaaaatat gtgtaacacg 147660attggagaac attagcagtc atttaagaaa atggacattt ggaaacatca ttttaagagc 147720ttgggatgaa aaactttgct atgacctata catctggatc attccactga ctgacactga 147780gagaaacagt gtattatcac agtttacaca gcaaattttt ttggagggga gaaggaggtg 147840attgaaatag aaatgatatg aagtttaaga aatacaactg attagatgtc acatgtcaaa 147900gacactgtag gtacccaata aatactgtta aatggactgg acaagtggga tgagaacact 147960ttttccccac acctctgact agtacgtgcc tttcttagga agttattttt tcagccatga 148020aaaaaaaagg gcagaattta ggacagataa aacacaagta gcttataaat cctctgaaag 148080gaaatcaaaa cacagtattt tgtatctctg tataaagtta agctcctcag ggacaggggc 148140aatgtccaga ggatggtgag catactactt agcaaactaa tgcaggttaa taaatattga 148200cacagtaaga cttgcatagt ttacattcta attttgtaaa gattatgagt tacctcttca 148260tattctgttc ttgtatttct cctgtgtata ttataaatac acaaacattg caaattattt 148320tttgaagcta tttcattttt ggtcaagact attagcgttt cttaaaaggg tttctggtca 148380tccttagggt ttatcagtca catcattaat attgtattca tttcactaaa aatgtggtaa 148440gtagcttaga tttaaaaaca ggatctaatt tcaaagctat ttccactata cataccaaga 148500taaaactttc ttctctctcc taaattctaa atttcacatg atttaccaaa tcacctctta 148560agagtactta ttggtcagca gtgtgagaca aacacttacc ccacttgtcc aatgtattcc 148620acaagattga ctgggtacct ttgtgtctct gacaggtaac ttccgacagt tgtatttctt 148680aaactttaag tcgtctccca aaaaactctg taatttgctg cttctctcaa tattgcaggc 148740agtgaaatga tccaaaatta cgcgtcatat ccatattcct tcatgattga ggttaactat 148800gcactgcaaa ccccatatgc tattacttta tattttcagc agcaccctag cctcttaata 148860atctcaaaca gattttgtac tgttactaat ttagcacatc cataaaaagt cattaaaaaa 148920atttagacgc taaattctct ttcaaggttg ccaagcttca ccactgtatt aaaattattt 148980ttaaaatttc aaggactaag gcatttggta cattcaattc cacctttacc aacaaaagcc 149040tttatcacta agatgaggaa agcatccttt tcattttctc cagtgaaaag ggggaaaaaa 149100aaaaaggcca actgcttgca tgcaggtctg catataacac agcagtctct ggcaaagcca 149160gtaactcacg ttcaacttca gaaatcctgg tggacggcaa cacatgagta atctgtgcgc 149220acaacccttg ggcttgacat accacttcct ccgccctccc ttcccatcaa tccaccccca 149280aaaccccaac ccaccaagca cacggctaaa tatgtcttcc ccaagagagc cagaaacctc 149340gaaaacgccg agaaagagct ccccttggaa ggaaagggcc tgcccttccc agtccggggc 149400cccggctggc tcgacaagcc tctctccgag gaggcccaaa tctcgacgcc tcgcacgtcc 149460agccccgccc cgggcccgct cgcgtcccct cgggcgacaa aaccctcccg ccggcgctca 149520gccaggactg acagttaaga ctgcagcagc cgcggccacc aggggcctac ttctggcacc 149580agcccctccg gaccacgcgc ccccaaacac tcgcctcggc ggcggggggc ggggaagcgg 149640ggcgccgacc tcgccaccgc gggccccgaa ggccggccga gtgacggagg ggagccagcg 149700ccgcctggcg caggccgcgg caacactgac ctgtcgtcgc ccccgcgcct gggctgcggc 149760ggccgcctca ctggtcgtcg tcgccctcgc ccgccgcctc cgcccgccac cgcctcgttc 149820cctttcgctg ccgccaccgc cggggctcat gcgggacccg cgcttcctta tccggaaaac 149880gaggccggga aagggggcgc cggagccgcc gaaattccgg cgttcgtcgc cagccgccgc 149940tgcctgtcca caccgggggc tgcggggccc tcctgccagg ggctacaggc cgcgagctat 150000tccgaggagg agccgaagga ggcgccgccg cccgcagcag agcgcgccgg gtcggcagag 150060gtgaagcgta agtaggcggc ggatggcggg cgcccgggct ggtggatggc tacgcggcgg 150120aggggtgcgc gagcgagcga agcctggtgt gtcgggtcct ggcgaaggga aaaggccctg 150180tcgtctcctg tagtcacccc gagcccagcg ccaccatctt gggagaaaca cggatct 1502373113476DNAMus musculus 3atccagggca acagggttac aagacgtccg ttgactcctt ggggggctct tgggtgactt 60ccctgaggtc ctcagatgcc cttgccctgt ttcccataag aggaaatgtg gacatctagg 120atgcacattg gttggcaaac attccattcc aacaagcacc tagttctaca ttctactctt 180gggaatttct gcaaggcctg cccattgctc atgactgtct tttctggtct tttttgcagt 240catatgacat gccaccttgg aaagcagaga accacagata tcaggtatgt ggaaagatca 300tggctaaagc ctccccttcc atatgcccag gcccttcatg ttttggtttc aattctgagc 360caatcggccc ttttggaatt taggaataag aaaatagtat gcaatcacca aacgaagctc 420gatttaggct atgttctccc atgtagatcc ctagtttgca aagttttaag gaatttgttc 480aactcctgat aatagatttc tcataatttt gacctacttc tcatggagag aagatgacat 540ttttagtatg ttgggatctc atacttggat tcttatgtgg ccatggtgaa taggtacagg 600atgacaacac aagcctccag ggccatttag gactcttcct ttcccatgac acagaaggcc 660caagggacag agcctttctt agggagagag aaggattcta tgatatccaa caaggctggc 720ctccacatga agtgttcaca aagagcaatc cctttagcaa atattccaaa gatttcaacc 780cacagggaat tcacccatgt ggagagggtg agattttcat ttttgccttg gtttccacca 840ccttccaaat tttcttcatg gtatcatttg gggctcttac tgaacctcac tgcagaatca 900ctgaggaaac aatggcctat acattcggtt gatacaggga aggccaggtg taaaggaact 960taattccact ttgattcatg acaccaaggc ccaaatttga tccagcagta gtggttacct 1020tcatgtgtta gaggctgggc aacccattac agcttaacac aaatggatcc atggctagac 1080atttgtcctg tgccctaggc ccaggattct cgggagattt tcagtaatca catttcacag 1140tgagtgctgt catgcaatat agagcttaca gagttctgat gccacctttg aactttgaat 1200aagaaaccat tggttacttt aatcacactc gaaataccta tctgtagttg tgttttattg 1260tcctgtccat agttctttgg gagctcaact tgagtgttga tccccacaaa attgtgaggc 1320taaggtcaca tgtgatgtgc ctattgcttc tagaggaagc agtaaagctt tgttggaatt 1380ccaggggtcc tttgtgcttt taattaggtt tctctatggt atgcatgaaa acttgtgtgt 1440gctgggcctg gttttagtag aagaggcagc cacaagagag tttctgcaca atggcgttct 1500gagtgtttaa taaccatact tggaggctaa tgttcagagg cagtgaatgg gagaatattt 1560tttcttggcc ctccaaggtc attaagtccc aaaggactgt gtgttatctc tagaaatgtt 1620cacacactgt ataatgtcac ccgtactctt gttctaggat gctatggtga ccccactaaa 1680tgaattctaa ccctttgcat gatacagatg ctttgcatct tttaattttg attgtggcct 1740cttagagaga ttcttttctg ggacgtttta agcaatactc tttttacact atgcttcttc 1800acaccttgaa gcactttaga aagatttata cccaaaaact tttaaaagca gttcaggtga 1860cccctaaagg gaggactaaa gggccatgag ctattcatcc ctcttcttga caccctaggg 1920tgcagaacta aagagctgga ctgaaggctt ctttgtataa tcagtaatca gaaaactttt 1980tttaaaaaaa ttaggtcaga taacatcttt tggggccaat ttgattcttt ggctgttgta 2040gtactccaaa actaaagatg actagatctg actactgcta aggataggat ttgtgcccta 2100cttcagaaat tatactggtg tgagatggag acaaaaatcc aatggcatgc ttcctcctat 2160ggatggccat ttgattcctg cctactctgg ctcctctcct ttgtcatttt tatcttaatt 2220ttcaaccatg ccaatatttc taattacaac acaaaatatg aatcctaaaa ttttaaggat 2280ccaagtcatt ttaagcacaa gtatctaaat atctatataa cttataataa gatactttaa 2340atcacaaatg tatgacatca ttttgttgtt ttcaaccaca gtgaaatgtc acataatatt 2400gtgttaatat taacccacaa taataataat ttaacaccat gcaataataa attcatatta 2460agaaattaat ctggacagat ttcatagcat gaatttggat aaatttttaa ggaaactgct 2520ctgacatatt ttcctaactt ttaatatgtt taataggagt ttctaaaaat gtttatttct 2580aaatagtttg aatatataat tggtgtatga gttttcttat taattaaatt tgttctttga 2640caatttcatg catgtataaa atacatttta ataacactta tttacctctt ccccctccta 2700tcattgtcaa cccaccctct ttaccacaag cctctttcct gtgtttatat ctttctgttt 2760tgtatgcaac tttgctttgc aagggtcatt tgtgtatcca tagacttcag aattatccat 2820tgtagcctca taaactcatg gatataaaaa taatgactct ccttccaccg tatacatcat 2880taccaatagt ttagcaaaag gtaggtcctc atgagtcctg cccccaacca tagatggtta 2940taatagttcc agttttatac agaatgtaga tagccactgg tactatgagc taatagttgc 3000agtagattca ttacatctag gagaaagtag ttcataggtc ttatctcaat ctcctggttc 3060ttacattctt tttactcccc tttatttata caggatggta taattacccc tttagaattg 3120agtacttaac tacctgtttt gttttacctg ttttgtttac ttgttttcat gaccatgcat 3180gcttggtaac aaacaaccaa gaaagaaaaa tatgattaaa ggtcccaaat tgagatgtta 3240tataacacag gacataaagc agtactcaac cttcctttct gatgctgtga ccttttaata 3300cagttcttaa tgttgtggtg acacccaacc ataaaactat gtcactacta cttcatacct 3360gtaatttttc tatcattatg aatcagcttg taaatatctg atatgcaggg tatctgatat 3420gtgagctcca aagaggccat gacccacagg ttaaggacca ctgacataaa cggttctaat 3480acagaatgtg tgtgccaaaa tatagaaata tgaatggtat acaacatata cttattattc 3540tcatgtaatt gaaattttct ttgtttagta ttaataatta catctttatg gaattttctt 3600atgagtgact gtgtgtgtgt gcacgtatgc atgcaggggc ggggcatatg tacatgacag 3660tgacgaagaa tatcaggctc tcagtgtttc ttaggtgctg ctgaaactgt tattttctta 3720aatctgtagt aaacacataa cttaagaaca taatattata atatttcact tatatttcat 3780aattactctc taagtatata ttacatatgc tataaatcta aggtaatttg taggcctagt 3840gaaacagaca tcaataagcc taatgccctt ggttaggatc aggaataggg aaaacataca 3900tggcaagaaa tccttcaacc tttattgaaa gaacttacct attttttttt tataaaatag 3960aaatactgga tattgtcctg agttgcctct tgtaacacag tctattgatg tgaacatggc 4020aataatattg tattccaatg tgaaaataat tccttagttt actgagaata tatagagatg 4080tttaccatta gaaggtttgc attaggttat tttggtgttc atgtcttttt gttccagtca 4140tttaagtgat aaatgcagaa agtttaatct tgtagcaata tttattcctc ttacttttta 4200taaatgtcaa attgtcccct ccaaaacttt ctttccccta ttatcatcct actacaatgg 4260ctcagctaaa ggtcattaaa ttgacctatt cctagctttg ataaactaaa aactattttg 4320catttttaaa tatgattttg aggctgagtg tctatttcag agtctgaaag cacacctaat 4380cttgcgcgcg cccaactggc cagaaagaac gacgctgcaa caggatcctt ctgcacacgt 4440ttattcagtc ctgtttcttc attctctata tctcccttgt ttatatctcc cttgtttata 4500tcttcccctt gtttatatct cccctctcga ataataattc ttcctctaac ccgggcctct 4560cactcttata tactctcagc cctcatccat gcacagcagg ccacgccacc tcaccaggca 4620cgcagcttca gctaatcagg gcagcagggg catatctcca tcaaaatgga ttcaccagta 4680tcctggtaca cctgtgcagc tctcaagatg tttgtggctt atatgaggaa gtcaggtgca 4740agccatatga cttagctgca gtccctggag ccttcttggg attgccccca cacccgctcc 4800tcacaaccta atatgttcca agccatatac atatacatgt acatgtacat gtacatgtac 4860atatacatat ataataataa ctgtcttttc tttactgtca caatgaataa tggcacatga 4920aaactatgaa acatttgttg aatcaggtaa ataaataaaa gctactaatg ttctctttct 4980aattatactt tcacaccttt tgactcaaaa aaaaaaaaaa cccaaaacct attttgcaaa 5040tcctggcaat ggaactgtag taaattaacc cagttataga agagctaact tgtcaggaac 5100aggattacca agatcagtga taaacaaaat ctcctaaaga ttgagaatta tttcatgata 5160ctaatctaca tgccctttga tagcaaaata ggatttccag aattatttaa atgtgatatc 5220ctctttacta aaaatgttcc tacataataa tttcctacac aatagtttcc ttactaaatt 5280tcattatcaa aatggctcca atgggcagac attagcctta aggtttcaca ttaaggagaa 5340gtttgatatt ttaattactg tgaacagttt gatattggtt acatccaagt attgcgtgaa 5400tacttggtta cagaaatata tagaaataaa acatgatcaa ctggtaataa agatgaacgt 5460aactatcagt aaaattgaga aagttccatg ggaaaacaga tacaaattat acctaaatac 5520aactgtatta gtttattata atagctattt attcttttgc aaattgattt ctttaatttt 5580tgcttttgtt atttctgatg aataaaatat cagcattagc atcatgttta gagagagaaa 5640tcagcatatt cctttgtcat tacaagttgt tataggctaa gatgatcata aaattagttg 5700acacatttaa aatgagtaaa atgtgtattt atatatgatg catatatatt aaatatatgt 5760atatatttta tacatttaaa atgagtaaaa tatatgtgtg tataacacat atatagtaat 5820atattaccat atataaaata agtaaaatgt gtgtgtatat atatatatac atgtatatgt 5880atatatatat ataaaatatc acataggtag atgatagatg gatagataga tagatagata 5940gatagataga tagatagata gagaaaaagt gtgtttaagc taacacactc agaaatagat 6000gaaaacctag aaactagggt aaagattaac ggctgaactg ccatttatac ttgctcaagg 6060agataaaaat cagctttctc cagtgaaggg acactggata tatcaacctc tcaagggtag 6120gcctcatgtt cagtagtagc tgaccaacat ataagagact ccacagtttt tttgtgtact 6180tgcatttggt tacagatttt atttttcttt ttgaggtatt gtttcatttt gttttctcag 6240tttacaatgg ttgttgtatt aatttttttt gttggtttgt ttggtctttt tttgaaggaa 6300cttaaatttg agtgggtggg taggaaaggg gaaaggatct ggaaggatat acacaaaatt 6360aaaaagaact aatcctttaa gaagataaat aacgctaaca aaatgaatca atgattttaa 6420aagatacaaa tacatatatg aagaaatatg agaaaggata ctatagatta tatgtatatt 6480aaataatagt aagagaaatt tatgaatgtg ccaatgaact cagcaattta atgaagtaaa 6540tgaatgagtt gaaagattca aatataaaaa tttatttagt acatagataa cagaatactg 6600ttttatcaac aaattaaatc cttattgaaa acattcctac aaagaaatat agattacaca 6660gaattttaag attttatcct catatcacct tctagaagat ataaaaaagg aatagtgcca 6720agctcaatat atataaaaaa gtaacacctt catcaaaaaa tgctacattt aaaatgaaga 6780atatacagca atatttttaa tgagtataga tatatatctt tggtcaattt tttataaaat 6840agcatctaac aatatgcaga aatatttatg attaaatcaa attaaccatt gaatgaaatt 6900tgttttacaa tttcaaaaca aatctatatt taaaaaatca ctagacagtc aaggtaataa 6960aaacattagg aatgtatcct tccataaaat tactatttac atgaatattc aaaataagcc 7020aggtataggg atgtacatgc aatctcagca ctgagaaagc cataaagcag ataaatgttt 7080atttcaagac cagcttgtac aacaagtaag accttatctc aggcagaagt caaaattaat 7140atttggaatt ttgtaaatta accaaagact taacaaaatt acaaagaaca tttatgcaag 7200aaaagtgtct ggctctcagt ttacaaaaag cagaatttgt gttactttta acatgtcttt 7260atttcatact cctccctgag acactgtggt agcattgtaa atcagcaaaa tagaagccat 7320gagagaagaa gcaaagattt gtaccccctt cccatcctaa tccccccaaa ataactattt 7380tgacctgcta agcagttctg tagaaaaata tatcccatga tatttttagt aatttgggct 7440gatgcagagg cttatgtgtg agtaaacccc cataccagca ttaagcataa ctaattaata 7500taagttattg aataccatag aagataaact agttaacagt agctgagata aaaattttta 7560aaattatgaa actgtaaaaa caatctgtct ggtaaagaga aattttgtgg tataatcagg 7620agttctgtgg aaaatgttac cacactgctt ttggtattga gtccacatca atattcagag 7680tgcatggtag catatgaaac atatgaagtc cctagttgct tacttcttaa ttatcactag 7740gccctgtgta agtagtaagt tatggctaag aaacagttgt gaactacctg aataccacat 7800gtgaaccaaa atacacatac agaacatccc ttaaaaataa tgagtgacat attgtttaaa 7860gttatttaaa gaaatctcta tctaatcatt gcatgtgctt aaaatggaaa atacaattca 7920gtatccacat acaacaacac acattcttta tgaacaaaaa taatgaaaaa gaacagatca 7980accgttaaaa caaactctag gaatgataaa acatattttc atgcatatac attatatgta 8040aaaatgtcca gtttcaaaat atatattgtc ttagttttgg ctttattccc atgtaaagat 8100accatgacta agacaactct tataagggat atttaattgg ggttggctta caattctaga 8160ggctcagtcc attatcatca tgccaagaag catggcagtg tcctggtgca gaagaaagag 8220ccaagaattc tccatcttga actgaaggca cagacaggaa gaatgtctct tccacactgg 8280gaggagcttg agcaccagca gccctcaaag cctgcctaca caatgatata cttcttccaa 8340caaggtaaca cctattccac caatgctgca tcccctaatt ttgccacttc tatgggtcaa 8400gcatattcaa accactatat gtgtgtatgt gtatgtgtgt gcatgtgtgt gtgtgtaagt 8460ataaaaataa gagattaaaa cacatgtaca gaaaaagata tcaatagaaa ctggaaacaa 8520ttcagatgcc ctcaaccaaa gaatagatac agaaaatgtg gtacatttac acaaaggaat 8580accattcggc tattaaaaac taggacatta tgaatttcac aggcaaattg atggaaatag 8640aaaatatcat cctgagtgag gtaacccaga cccaaaagga catccatggt atgtactcac 8700tgataggtta atattagcca attcgttcag aatatccacg atacaactca cagatccaaa 8760gaagttaaac aagaaagaag gcccaagtat gcttgaatcc cctaaaaagg tgaaacaatg 8820cagtaagaga ggttagaggg aaggagaaac ctggctcaga gaggggagtg tgaggggagt 8880gaaggggggg gcaggatcag gcaggaggac agacaggaaa gaagcccaga gggccaggag 8940agtcagtgaa agtatgcagc tgccaggtgt tgaggttgga ggattgtcta ggaagtccca 9000gagacctggg atgagggaac ttcctgggaa tcaatgcaga caaccttagc tgaaatgcca 9060aaaaaagggg aataggaaag ctgatgagac aacatccagt agccaaacag gactcccagt 9120ggacggatag ggacactaac acacccacaa aactttagat ccaaaattgg tcctatctaa 9180aagaaacgca gggaaaaaga tggaacagag actgaagtaa tggccaacta gtgactgacc 9240cattttgaga tgcatcccat gagcaggcac caatccctca tgctattaat gatgctatgc 9300tgttcttgca gacagatctg agaggcttta cccatcagct gactgagaca gatgcagata 9360cccacaaaaa cggaacagag gttggagaac cctatggatg atttagggga agaattgaag 9420gccctcagtg aggtggtagt cccatgggaa gatcaacagt gtcaactaac ctggaccttt 9480gggatctccc aaagacagca caaagctccc aaaggcagct ccaatcaaaa aagcacatac 9540aggctgatct gaggcccttg gcacatatgc agcaaagggc tactatgtct ggcctcagtg

9600ggagagaaaa tgtctaatct tgtagagact tgatgcccga gggtggggcc tgaaaaggga 9660gggctgctct ctcagagatg tgggggaggg gaaatggagg ggaaactcta caaggggggg 9720ggggagtcca ggagagagag cagcatttga catataaata aataaataaa taaataaata 9780aataaataaa taaaagtatt cttaaggaaa gaaagatgtt ggacctacta gacaaaggca 9840gtaagtcaac ttttaaaaat atattcaaag aactaaagaa aatgaggaaa tatttacata 9900atatatgaaa atgatgcttc aagaaatata gactaaaaaa cagtaagaaa tgaccacatg 9960aaaattactt atgttaaaaa agtaagtgtg taatgtgtat gtagtttaat gggagaaggc 10020ttgaccagca tattcaaagc cctggcttcc atctaacact gggaaaaaac caacagaaat 10080atacatgata taaagaactc tcaataatga atgtcagcag accagatatt ggctaacatg 10140aatatgctct aagtaagatt atctagtcaa agtacgaaac agaaaggaaa aaaaatagct 10200aaaatataga aaaataaact aaacaaaata gagccatagc aaactgcatt tatatcaact 10260tatacattaa tgtgagtcca agaaggaaaa agagaaaggg gaaattttta agaatgatgg 10320ctcaaggggc tggagagatg gctcagagat taggagcact gactgctctg gaaagaagtc 10380ctgagttcaa ttcccaccaa ccacatggtt gctcacaacc atctgtaacg ggatctgatc 10440ccctcttatc aaagataacg acagtatact catatagatt aaataaatct aaaaaaaaaa 10500ggatggctca aaactttcca tatatatata aaaccaatgt accaaacaag aatataaata 10560agcttcaaat aagataaatt caaatgcaat catataattc taaagaacaa agcatagtag 10620tgaaactatt gaataattaa taaaaagaga atgtgaagaa tgtgtaaata tcaacaacac 10680gctcttcatc ataaactatg cagacgagca ggcaatataa catatattat aatattacaa 10740tataccataa tgaaacaata tttaacaatt taacatctat gaaaatacac tcatattctc 10800atcaataggt ataatgtcca gacaaaaata acagaaaaaa ttcaggtttg aactgcatca 10860tcagcccagg atgaaatgaa tggataagct gctgaggtct atcagataat taaagagtca 10920ttatcgccaa tatctccaaa attattctct aaaatataaa gttcatcaac agtaacataa 10980gttatgagta cagtattaca ttgatacaaa acttaaataa aaatacagtg aagaaaaaaa 11040tctataatgc aattgttctg aggagtatgg atgtaaaaat tcttaataaa aatgcttaac 11100caaatccaaa tatacatctt aaaagatcac atactaatac aaaagttgct tcattccaga 11160gataaaagga taattcaaca tatataaggc agtaaatgta acaaacatgc acacatatga 11220aacatagatc aaaggacaga aattgcatga tcattgtaat aaatacagaa aacagcctta 11280gaataatgaa taactgttca tgagaaaata ctccaagtaa actatttatg gataaaaaga 11340caataccttg atataataaa ggccataaac ataaaattat agcaaacgtc attctcaatg 11400ggaaaagaac tgacagcatc acttacaaag ttgggaaata agtatatctc gtctctccac 11460ttgtcaatat aatgcttaaa ttattcacct aggtaataaa gcaatggaaa tacagggtac 11520attaagtgaa ataattcaat ccattcctat tacagacaat atggacctac actcactggg 11580cactaaaatt ctcctcagac aggaaaacca actgatctga taaatgctcc taggaaaata 11640gcaagatata aagtcaacac aaaaaggtat gacaacaact tttctatatt tgcataatga 11700acattctgag cataaaactg gtcacattta caggaatgtc aaaacataac cactagaaat 11760aaatctcacc aagaaggtaa atgatctcaa caatgaaaac cttaaaacac ttccaaaaga 11820attcaaagac acgtggtttg taaattgtta ttttgggtat tctgagtttc tgggctaata 11880ttcacttatc agtgagtgca tatcatggat gttcttttat gattgggcta tctcactcag 11940aatgatatcc tccagatcca tccatttgcc taagaatttc ataaatgtat cgtttttaat 12000agctgagtag tacattttct gtatccattc ctctgttgag ggacatttag gttctttcca 12060gcttctggct attataaata aggctatgaa tatagtggag catgtgtcct tatgacacgt 12120tggagcatct tctgggcgct cagtattact gggttctcag gtcctatgtc caattttctg 12180agaaaccacc aaactaatga aactcaagaa caaagaacaa agtgtggata ctttaatcct 12240tcttagaagg gggaacaaaa cacccatgaa gggacttaca gagacaacat gtggagcaga 12300gactgaggga atgaccatac agagtcgcac ctgaggatcc atcccatata cagccaccaa 12360actcagacac tattgtggat gccaacaact gcttgctgac aggagcctga tatagctgtc 12420tcctgagagg ctctgccagt acctgacaaa tacaaaagtg gatgctcaca gccatccatg 12480ggaccgagca cagggtaccc aatgaaggag ctagagaaag gacccaaaga gttgaggggg 12540tttgcagccc cataggataa acaacaatat taattaacca gtactcccag agctcccaga 12600aactaaacca ccaaccaaag agtacacatg gtgggactca tggctccagc tgcatatata 12660gcagaggatg tcctagttgg acatcaatgg gagaagaggc ccttggtcct gtgaaggctc 12720tatgccccag tgtaggggaa tgtcagggcc aggaagcaag agtgggtggg ttggtgtaaa 12780gggggagcag gaaggtgagg gggagggtgt tttggagggg aaaacaggaa aggggataac 12840acttgaaatg taaataaaga aaatatccaa taaagtcaaa gtcaaaaaaa aaaaaaagaa 12900agaaagaaag aaaagaaaag aaacgattaa aaaaaaaaaa aaagacaccg gaagacagat 12960tggtagagtt aataatgtaa acatagctct gcaaattaaa gtgattcaca ttttcaatat 13020aatccctatt acaattctgg aaattgttgg aagaattatt ttaaagttta aaaacaacac 13080tgagtacaaa aacctatgaa taaccaaagc aatcctaagc acaaacagca aagttggtat 13140tgtcacagta tgtaacttca aacatagtat agagccattc tgataaaaca acatgatact 13200taacacaaaa cagacacatg aacaaatata ctaaattata ctaaataaaa caaaacatat 13260aatcccatgc tgttgtgccc aactattttt ttgttttttt tttttttaca tgagtctcaa 13320aattatacat tcaggggaaa acatctttaa taaatggtgc taagacaact taacatatat 13380gtacaagaat taaactaggg ctgaagaggg cttagcagtt aagaacattg agggctcttc 13440cagaagtaat cggttcaatt cccagaaccc atgtaacaac tcacaaccat ctgtaactcc 13500atttcccagg gttcagatac ctctgcaggc ttccttggga agccaggtag ccaagtgata 13560cacagatata catggtcaca aaacactcat acatacaata ataaaaaaat taaaaaagga 13620taaactaaat tcttctctct taccctgcat aaaaatccac tgaaaacaga ttaaaggctt 13680tatttaataa aggctttatt taatgtatga cccaaagaat tgaagtggac tacatagaag 13740cacttcaaga tatagaaagg caaggacttt ctgaaagtag gtcagaaaat gttaggagga 13800atttacaaaa taggattaga ttacattaaa agtttctgca cagcaaaaga gacaaagctg 13860tagagattgc tcagctgttt cagttactta ctcacataag aactaagttt ggctccacat 13920ggtagcacag ttacctctaa ctccacttcc aggcaatctg ttgcccccag ttctctgcgc 13980acacatggta cacatagcct cataaaggct ccagacataa aaaaaaaaac aaaaacaaaa 14040acaaaaataa ataaataaac ttttaagagt aaagattcag actacacatg gaagaaaatc 14100tatgctgttt tattttttta tatctgagag gggactaata ccaagaatat gcaaaatgct 14160ataattagat tatgctacct gaacacatta agcagttagg aacattgagg gctcttccaa 14220agttaatcgg ttcgattccc agaacaattg tggttaaaca ttgaaaccac aagtaatatg 14280aattttccat aaagaaaaag agatctttac tgataaagct caagtgacca ataaatacat 14340gaataatgat cagtattttt aaccattaag gaaatgcaaa tgaaaagact ggaaatttat 14400cttatgccca tcagaaaggc tacctctaag gaaataaact agactccttg tggagatggc 14460tcaggtagta tagtgttttt ctttcaagca tgagaacctg agtttgacca actaaactca 14520agtacaaaga agccaggcaa ggtggcacaa gcttgtaatg ccactgtggt agaggctgag 14580acaggtagga tctctgggac tcactgttca gatagcataa tctactattc caggtcagtg 14640agaaactcta tcacacaata aacaaaacaa aacaaaacaa aaacaaataa gcaaacagga 14700acaaccatca caaaacaaaa aacaagtgaa tagaaactta ggaaagactt ctagcctcca 14760tttgcacaca catggaaagc aacaacagaa aacactgaag aggatgggag gaaagaagga 14820ccactataca tagctagtga caatatgaat tagttcagcc atactaatca gtctgtatac 14880aggtttctca aaaaaaaaaa acaaaaacaa aaaacaaaaa aaacaaaaac aaaaaccgaa 14940aattacaact accatatgat ggagtgatgc cactcttggg aatgtaattc agtatagaga 15000tatgtgaaca tccttgtcca gtaaggaaca aattaaaatg tagatggtat gaattagttt 15060tgatgttcat gaacagatta atagacagct gggattttct tcatccataa agagtaaaat 15120gatcacattt atgaaaaaaa ttcacaaaca caaagacaca cacagactac acatacacac 15180acacacatga atggcatgga agtagggaaa agtattcagg aaaggcaaag agggtgagtt 15240gtaagaaaga agatcaataa aagatcataa tgagtgggtg atgaatgtga ttaaagtaga 15300acatatacta tcatgaaaat gcaatcgagc aactggagag atggctcagt ggtttaaagc 15360actcactact cttccagagg tccttagttc aattcgcaac taccacatga tggctcacaa 15420ccatctgtaa taagattcca tgccttcttc tgatttgtct gaagacaact acagcatata 15480tatatatgta tatacatata tatatataca tatatatata cgtgtgtgtg tgtgtgtata 15540tatatatatg tatatatata tatatatata tatatatata tatatacata cagagagaga 15600gagagagaga gagagagaga gagagaataa aataaaagga ccaggagtca gaacagattt 15660ggggatgtaa acaaacaaat gaataaatta aaggataaat aaaatgacat aaagcccatc 15720actttgtact agaaatatat gctattaaaa caaaaagaaa tgatagcatc tagctgcata 15780ttacatttct gtagtgaata actatagatg gtgtgatttc ttaaagtcag ccacacatta 15840cacattagtt gcatccaaga tgctaaacat attagaattc gtgtaatttc aacttactga 15900gtttaaactg gttagtataa aagtcaacca tttcaatgaa agttactata ttttatgtaa 15960ataccttttc ataattaaaa tctattgaca caattatgga aataaaatta aagcacttag 16020cacaagctag catatacaca cactttccac ttacatgtgt gataaggtta aacagtgaga 16080acatcttgtc tttgatttct gtttggaatg ctactggttc ttactctaat ttattcaaca 16140aatgtacatt tatttaaatt tccatttctt gtgtgacttt tatcagagca attgatcaag 16200gaatgggtag gttattaagt tggacacaga aatgatcatt attattttaa taaaaaccat 16260tgaatacatt taaagttata actacatcct tacaaaattt atggatcttt tctaagaccc 16320aggttttatt ttacatattt ggtttcattg attactgtta ataatagttt ctttttttgt 16380tttttattag ataatttctt tatctacatt tcaaatgtta ttccctttcc tagtttcctc 16440tccggaaatc cctaacccac ccctcctgct ccccaaccca cccactccca ttcccagtcc 16500tggcattccc ctatagtggg catagaacct tcacaggacc tattgcctct ccttccattg 16560atggccaaca acccttgata ctttcctact gttgccttct actaaggttg tgattttggt 16620ttttcattgt aatgtatggt gttgaaaaga agaaaaaaaa tgagtcttgc tcatttttat 16680ttacatttgg tgtgactgga tgttcaaacc agtggcactg aggtgtttct ggttaaacaa 16740tgttggctag gatcatctaa tactaattct tattgctttt gtgttgggat aagttgtggg 16800gggtctcaat gttccctgtc acctccattg atataactat gataggaaat gaaaaaatca 16860gttctcttct tggtaccact tgcaggtgat ggaagttcat ctggcacaag cctggcaagg 16920aaagtgatat actattttta ccaatacatg tattttccta ttccatgctg tcctttctta 16980taaatcataa acatggaggg gaaagtgtta ccgttgcttt gcagacacta taaatatatt 17040taatgtcacc taaaatagaa aataacttag gacacaagta gatattatta gcagaatatg 17100acatattgta caagtactag tcagtatagt aagtaatgaa aaaaataaca gacttgcaaa 17160tttgaaaagg aggaaagaca tttattcata gatgacatgt ttatctataa aaaactgaaa 17220accaaaacaa gaaccctgag attcaactag aaattggata tctaacctat aatcagagca 17280ctgaataggc ttgtctcttg ctaattatct ttgagataca tcattctaag aagctacttg 17340tttaactagg ttacctgcta aagttttatt taaacaccat caatgaacaa agactcttta 17400ttctttttat cagtgacatc acttaagtac agacaatata atacttaata aagtaccaaa 17460tgcagagctc aattttggcc tgttaggtta agtttataga taaacatata atactagtag 17520aatgattata tatgtcttct aggaaatcct tacctactgt ttcccaaaat taagaaaatc 17580ataccatatc acaagattag acagttgaca agaaaaacat attcttatca agaatattgt 17640ttatgagtgc ttatttactt ggacaaaagg tatagtaaat ggcttctcat atgaaaattg 17700aattggctat acaatacaaa cataaaaatg gtacttttct ttttgaataa gttagactga 17760ttttcactca acctgtctaa cttgagaaca tttgacttac tggcaagggg ttccaaacgt 17820tagacaagat ggcacaactt tgttatctac aacattctca gttaagaact gtaacagaga 17880gagagaggag agagagagag agagagagag agagagagag agagagagag agagagagag 17940agagagagag agagggagag agagggaggg agggagggag ggagggagag agagagaggg 18000agagagagag ggagggaggg agggagggag ggagggaggg agggagggag ggagggaggg 18060agggagggag ggagggagaa acacatccta aaatctcatc atttaagtaa atttacaatt 18120ttgtgttatg ttgtatttat agcttttctt gcttcatgaa gtcttcttaa gcctacaagc 18180cacagtttga ctatacctga atagtttaaa cctagtgttg ccttgatggc tttctatcct 18240gataacctgt gcactctaaa gtagtgtata aagatctaag gctttactga accttagata 18300tctcacttct catcgtttaa taattgcttt ctacatttac acacaccaat attgggtgga 18360tatatttata attgttataa ggtaacttct gtaatccatt ttaaacttcc actattgtct 18420ccttgcctcc cctttgaatt tacagttcac attttaaaga atttcaaaac accccttatt 18480tttcattgta tttattagga tagctttggc caggtcaggg acatacatct ttatttatag 18540cacttgggat ttaaaagaag aagttcagat aaaccatgaa tacataatga aaccatgtca 18600caaaaaaagg aaaacatttt ccttgatgat attcacacta aagattgtca ctaagcctct 18660tactttatta tttaaatttg ggggtgttga gaatgaaata caaagcctta catattctac 18720acaagcagcc tacctctgaa ctttgcattt ggttctgttt ccactgtaat taacaatttt 18780ttgtaggact ggagagcaaa cctaggattt aatgcatcca aggtaagcac tctactgctg 18840tgccaaagac cctaccaatt taacatttta cataaggcag gccactttta tcacattaac 18900ttttagggga agagggagtg gtctgttaag tctcatgttt ctgaaggata gctctgttaa 18960gcatagtaat tcttggttgg cagcactaca accatgtaat aacataaata ttaattcatt 19020tatggatgac tgggacatag aagctctgac atattagtca attaagccta attgttggat 19080taatctgaag gcattattga aaaatggtag acagaacaat aggtcaccag gttgttgtct 19140gtagccatgt cttagacttt tcatgtctct tcctggttct tgaccactat gaggtgaaca 19200gtcttgcttc atttatcata tcttctgcca tgatgtcctg cacttgtaat caacaaaagc 19260aatggaagca gtagatcatg gactaaaacc accaatacca tgagtcataa caactgtttc 19320ctctttttaa attgttcaag tattcatcaa agtagacagc agatgcaatt tttttctttc 19380atttctaaag ttttcttttt ttttattaga tattttacat ttcaaatgct atcccgaaag 19440ttccctatac cctcctccca acctgctccc cgatccaccc actcccactt cttggccctg 19500gcattcccct gtactggggc atataaagtt tgcaatacca aggggcctct cttcccagtg 19560atggtcaact aggccatctt ctgatacata tgcagctaga gacacgagct ctggttagtt 19620catattgttg ttccacctat agggttgcag agcccttcag ctccttgggt gctgtctcta 19680aaaattcagg tgacaacaga tgctggtgaa gatgtggaga aagaagaaca ctcctccatt 19740actgggggga ttgcaagctt gtacaaccac tctggaagta agtctggcga ttcctcagaa 19800aattggacat agtactacca gaagatccag caatacctct cctgggcata catatatcca 19860gatgcaattc tttagccctt taaaaacttc tctacttcaa agcatgatgt ttctattgag 19920gtgtatgcta catatacgag agggctctta tatatggttt cttttgtttt caacattttt 19980ctggtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tatgtgtgtg cgcgtgcatg 20040cgcagaggcg cacatgtata cctatgtgtg tggcacaagg caatgactat gttctggggc 20100aaatacatag accaaacctg gaatcccgtg aactacctgt acctgggtaa ctttcaccta 20160gtataggatg tttaatgcca ttaattcttc agcctcttaa acctttctct ttgttttatc 20220ttgaacacaa actatttgaa cctttgtcct attgtaacat tttgtgctat tgattcaagc 20280cagggaattg ggtaagcaaa gcaaatgagc caacaccaag ttaaaaaaaa aattcctttt 20340taatcgtgct ccaccaatgc cacaaacttc cttcattcat tctttatttt tctcttcttt 20400ctatatattt tcagataact aataatctag gtaagagttt ttatttttga ccaatgtgtt 20460atttaattga tgttttcagg tctaatattt gttgctattg ttccaatctt ttaaccttcc 20520agtcatgtgt tatttgtttt gaatcctcaa tcattatgtt tgaatatcag ttaaatttgc 20580agtttggtaa tctgatacat atttttatta cattcgccta attgctcaga tacttcacac 20640atttccagtt tttccttatt aactatttgt tctttccccc tatattataa tgattttgag 20700cactcttttg acccttgtaa taatgactta gtgtgtacta aagtagatac tcattccaag 20760ttttttcagt cttcctttgg aaaatgcctg ttcaaagacc ctggacagag tgccacaacc 20820tttgagccca tgaccaacac agcctttgaa acactaggga attctctcac tccaactcta 20880cactggctag cacaatgctg gactagaatc ctgtggctat tgatacaggc agagatttga 20940gaagcactca aaacctagag gtactgatgc taaaacagca ctaaaatatg cccaagttcc 21000attgccacca agccccttgt agtcatccag tagtgtgtaa gagaacacag accaattcca 21060tctggcagtt gctacatatt cttatgtgat atgagtaaac tgttgcagct caaatcacag 21120gagggtcttg cttcagaggt catactattc tgatcaataa aggtttttat gtcatgccta 21180gttggtattg gggtccatag taaaacttac atgcttattt atccaattca ttcactagct 21240atcaaactgc atacatatac agttttgctt ataaattagt tggtggatat tttgtatgtc 21300tttacatggt gactattttc aagagtgtgg cttttaaaag ttggtagaaa gactgctgga 21360gaaatggctc agtggttaag agtgccaacc gttctgcgct ggagagatgg atcagtggtt 21420aagagcactg actgcccttc cagaggttct gagttcaatt ctcagcaacc acatggtggc 21480tcataaccat ctgtaatagg atccagtgcc ctcttctggt atgtctggag acaactacaa 21540taaatacacc ttttttaaaa agcacaaaaa gaatttttaa aaaaatgtgg aaagcttttg 21600tttgaatact tcagttcatt tggtgatata cttgctaggc aaataatcaa gtcataccga 21660ttctctttag tttactgagg aaccaccaac ttcttctttc catatcagca tgattttaca 21720ctcatattag tcataaacat tctatatttt tccatactta ccaatatatt tcatctttga 21780agagaaaggc catttagtgg gcataataaa gcatctgatt ttggatgtaa tttgcactca 21840actaaggaat agtaaaggag agcatccttt caagttcatg ccactaagta tatgtatgct 21900ttagcatcta accgtttaga agtttttgct tggttcattg ttggactcga ctgtttttgc 21960tgcagaatta ccatgtgaca cagctattcc atacactgga tctttatcag atgcctatga 22020tttggttgaa ccaaaaacct acttgctctt ttagtcttta tcttatacct gaaagttaat 22080tttaaaattg aagattacaa acattttgtg aagattgagg atattcataa tacttaaacc 22140ttttgttcat ttttagaaaa aatggtgatc tatacttact cttttttaag tgaaaataca 22200attttatata aatacttttg tttatttact gtttggcata caataaaaac attaaaatca 22260tcgagcctgg agatcagttt aattttaata ttatatacaa caattctagc tctgatccca 22320cataccaata cataattata ctcatatatt atgcacacaa cttatctata attgctacaa 22380gttttcttga atcaatgaaa aacggtgaaa ccccacgtac aggcagatta ttacctaatg 22440acctttcctg ttatttctat ttattcgaac tattgtctac tgacttttgt ttcagcttga 22500catactctac ctgttataat tacagaaaac gttttaagtg gaaacagtag attctcaaac 22560tcatatcaaa atagtgggct tggttatcag taatttgaca caaagtgaat gtttccttct 22620tggagttatt taagctcttt gttgaggtag ggttttcatg tagccttggc tggccataaa 22680catatgtatt caaaaaagtc cttgaacttc aatttttttc tttcagtttt gttaatggtt 22740acattatatt cttttggagc tttgatattt acagctttgt aaatactgtt atgttgcctt 22800agtcagttga ctaatcccca cataatattc cttgtttcct ctaagtacta atttttaaag 22860tctctacccc tttcccaaac ctgatatata tcttgctctc tgccctaatt cactttgtgt 22920tacatttgca tggaagggcc ctttttctca atcttttact gccaacctgt ttttttgttt 22980ttgttttcct ggttacttct gctcttttaa tttattgctc gtatatgtaa atgatcactg 23040acaaatgttt gctttatata gtttggtcct tgtgtgtctc ttttttttct tttttttctt 23100tttttaatct tttactgttt ggcttgcgat ttcctccaat acagccatta atactcatct 23160cttttttggg ggacggaggg taatgtcaca ttttggctcg ttgtttgctt ttgtgtgtgt 23220tcttcagtat tctttgtaga aaccataggg cattatatac attcttccta atataaaaac 23280aattttcatt gaagtactgc ataagggaca aatctaaaca ttgtataatt taaatttaag 23340catttaggaa atagtaattc aagttccaac acttgttttc taataccttt tctttgcttt 23400gacattctaa gagtgacaaa aaaagtatac cttgtaacta ttcaaaatga tcaatgaaag 23460gaagaaaaca gttaagttgt aggcctagga acatacagta attaaccata atgaacaaag 23520tacagatgaa atagatgaat aagctaagta cagtagattc agtacttagg atcactgatg 23580ttttatatcc agagaaccct acagtaatca tatccttcta aataattttt cacatggctt 23640ctatatgata cgatgatgca aaaacatttg agctataaag gacttggaat cctttcagtt 23700ttaattattg attctcacat ctaaacttaa atttgaactg acagattaat tgcaagaggc 23760ttcatagtgg actactttat ttcaaagtac cacaaattac acctttataa aatgtacata 23820taacatgact tttgttaatt tctaaaattc ttcagaaata ctgggtttac caaccaaact 23880gaatactgag gttgtttaat cttaaaccat aacctgagtc gaaattgttc cttccacatt 23940aatcttattc taattattta tttaaagcag gatcttacta agtagccctg gccggcctta 24000aacacaagag atcctcctgt ttctgcttcc cagtgctgag actaaagtca tgaactaacg 24060ctatctgtat tctcttaaaa aaaaaaaaaa aagacctagt agtagtgatt tctcaaaact 24120tttacttaag aattcctctt aatattgctc tcattctttt tagaaacttc cctggatata 24180aaacctcagt gttagtgttc tcatcctata cctccaccct tcactctttc tgcttctgta 24240ccctcctttc agttcctcct cttaactttg gacatcgaaa aaacaagcta tccaatcatt 24300taattggaga cacatggata ttagttgctt ctttttgttg ctttcaagat tctgtctctg 24360gattttggta agtctgatta gaatggatca tgataattac cttttatggt ttactctaga 24420ctatactgga tatcatatgc atgtacattt gtgcctcaaa aaaaaattgc actttttcca 24480gctattactc cttgaacgtt cactcttagg gaaaatttgg tgcaaataca attagaataa 24540taaaatcatt taaaatggga attttgcttt ctacagctct gtaaaggtta tttttctgat 24600agctccatag catacaattg tctcaaaact attgttctaa gggtcttgta catcctaacg

24660aagtgcttta tcaatgaact ataccaatga attaaatcaa ttataccaat gaattaaatc 24720aacaacccgg ataaccctac aatgacttgt tttagggttc agattgtggt atgttatgac 24780aaatgcttaa aacacatttt tttttttaaa ttgtcatttc actgttgtgt gagatgctcc 24840atgtcactta cagtagttat ccatggtgtc caagtaatta tgggtttatt gttgtcctac 24900taatgactca aagaggttat tttaagattt gcaacttatg caatgtagat atgtcgtttt 24960tcttgtagtc ctgttatgaa aggttctaga ttcttccagt aatctaggac tgctcttaag 25020tatatagaga ggtctgtcac ctgttttcct aattttttag ttagctgcat gtttgtctca 25080aagcgtattt gctattaaca tgctcaactt ttttgagaaa acactaagat ttgtttccat 25140cctttaaaaa atcatttttg atatgcattt aatatgtatt taatatgtct tatacattga 25200atagttaggc ctttatacaa atccaactaa aattttcttt gcaatgtaga gtttatatta 25260taaagtttta tcactttgtt attctgttca ttagcaccac tatatatttt cccgttgttt 25320ctgcttttat ttaaattgag cattgtatta gttccctaag gttgctgtat tactgcaaac 25380taagtgacta aatgacatag tgcaaaacaa ttcattgtgt taattctgga gattaggcat 25440ggtaacattc aaacaaacaa acaaaagtat tatcagggct ttacctaaaa cctgtatgag 25500aatcactgtc tcttccaggt tctgattttt ttcacttttt aaaagtaatc tttggtattc 25560ttcatcttgt agaaacaatg cccaatctct tgctccatat tactcatttt cttcctattt 25620gtgtttgctc tccttatata gtaaggacat caggcaatac taaattacag cttattctaa 25680agccacaagt ctcaattaaa tacaactgga tagatacttc caattaagta cctcttcaca 25740actgtcagat atggcaatct aaaatctata tgtatagttt accaaactta aaacttctgg 25800taatcttcaa aattatttgt ctctgacttt cttcctgtta aggagctttg atttacacat 25860gctacagaag aaagggccaa gttgttgctt cgcttgttac cattatggta gctagaagtt 25920agcctttgcc aacattccct gtaaaatagg aacctgaggt taattgtcat aagtacctaa 25980gagaaatacc tgatcaacgt ccaaattata tcttcatact ctgacagctg agctgagttt 26040aaaaaattat agtaccagtg tcttaccggg ttgacaaaat gtttttaggc atttgttgag 26100ttttagatat gagactctca aatttgagtg gttatatatt ttcataagtt ctatcaaagc 26160taattataac acaatttcaa agagttacaa gaagctattg actcactaga aatcctgcca 26220gcattctgct caccttcgtt aggtatccct ttccaggctc acctccattc ctctctatac 26280cataaaacac agaaaggaca aagctcagat tgcaataaga caaatgaatt gccttctcca 26340ttggcttttt catttcccct tctgattttc taggacaata catttattaa tagttaaaag 26400acaaggccag tgacatggtt tagcatggaa gtggacttgc tgctatacct cacaatgtgt 26460atttaacccc attagtcaca caatagagaa ccaactcttg caaaatagtc tacatatgaa 26520attccattaa gaatgatatt tgatatacta caagtggcag aaattttcgg ggatacacta 26580ataaaaagtg acaggagcac actgctagtt ttgtagagag cttgggacag tctgagatac 26640agaagaggaa tgggggaaag agtagggttc tgggtaaatt acacatggcg actatcagtg 26700gatcccacaa atgcgcactc gcacgtgcgt gcacacacat agtaacaatt ttaatgaaca 26760aagtataaaa tgctagtcct ccactagaaa tgactagtat tgtatacaag gatatcttcc 26820caattttttt tttttttttg ccataacaga catgtaactg aaatattacc ttttatgaca 26880gaaagaaaaa aaaagaggga aaggaaaggg gaaaggaaag gggaaagggg aaagaggaaa 26940ggggaaagag gaaaagggaa aggggaaagg ggaaagaggg aaggggaaag aggaaaggaa 27000aaagcaaagg aaaagcaaag gaaaagcaaa ggaaaagcaa aggaaaaagg aaaggaaagg 27060aaaggaaagg aaaggaaagg aaaggaaagg aaaggaaagg aaaggaaaaa ggaaaggaaa 27120ggaaaaagga aaaaggaaaa aggaaaggaa aggaaaaaga aaaggagagg aaaggagagg 27180aaaaaggaaa ggaaaggaaa aaggaaagga gaggaaaaga gaggaaagga gaggaaagga 27240aaaaggaaag gagaggaaag gagaggaaag taaaaaggaa aggagaggaa aggaaaaagg 27300aaaggagagg aaaggaaaaa ggaaaggaga ggaaaggaaa aaggaaagta aaaaggaaag 27360gagaggaaag gagaggaaag gagaggaaag gagaggaaag agggaagagg gaagagggaa 27420gaggaaagag gaaagaggaa agaggaaaga ggaaagaaag aggaaaggaa aggaaaggaa 27480aggaaaggaa aggaaaggaa aggaaaggaa aggaaaggaa aagaaaactg agttcatacc 27540actaagctca tcactaaaga actggaagtt taaagtgtta ctagataact aaagagtcag 27600caaactacag gtttatgtcc cctacaagtg ctgttatatg tgacatacat gacaatttca 27660gattgttccc attttattat ttcctcttat gagttggtta tgtagaaaca gaaaaagcaa 27720aaatggtaga gatgaaaagc aaaaaacaaa caaacaaaca aacaaaaacc ctaaaacctt 27780acataaataa tggtttacac atgcaaacta aagaacttgg aagaataagt taaaagttgc 27840atagtactga acagatggtt taaagttaca tataaaaaca tgatggattt acccactatc 27900atctaaggaa tgagaaacaa gctggttttc cttctgttgc tttcccatac aggtgtgccc 27960ctaaaaccag tagaggattt cgttttccct ccacagtagg aaagtgtaag gagtatctga 28020atcggtagta aagctgcatc caggatgagt cttcatacac acgaacaatc tttgtaatga 28080ttttttttct tagcttttta ttgggtactt ttgtagaaat ttgcatgtat ggtctggttg 28140gctacccagg atctttatct ttgcaatcat ttcatctata cattctttcg acagatgtat 28200tacagcctgg gaattgagga ttaaaaagaa aaaagtgaaa gaaaagttga aaccactcct 28260actctaggac attatcaatg agaaattgag gcaataacta aagcacatac acaaatatat 28320atataaatac agatgaccta tatgaactat atctattcag agaaacacag cataaagata 28380tattggtccc aaacactgta tttggtgttc ttggtgggag taggtaaaag atgcaagagt 28440tttggaaaca tcttctaaaa tacagttgca taataggcta acttctacta ctcaaaatgt 28500acatcatcta gttagaaact agaaccctct gcacaatttt atcttgatct acttccatcc 28560atagctagct atgtctgtgt caagaaacct ttttcatttg aatcaacaaa taaattggag 28620gggataggga tgtagctcaa aaaagcactt gtctagttta tatgaggacc tgtgctcttc 28680tctcacaagt ctcctaccca aaataaacca acttactact aagctctttc agatccactg 28740agaaggatca aacaactcta ttgaggcaga gtaatggtaa attattttaa gagttaatat 28800ttcaattatt tgaaatattt tgatttggct aactagattt cataaggtac atgtatatct 28860ggttatttaa aagctatgat aattgatgat aattgagact ggaatggaag gcatttagct 28920agtcatatgg gagattgtaa ggagaagaat ggaaatgaga ctctaagaca gtctgctcat 28980tgtatggttc caaaaagtat cttcatattt tgatgtggat ccgtaagtca cagttacaaa 29040ttccctaacc ttgcaaagct tggtatactg agtagaacac agggtcaaac aaaccaattc 29100ctttttgtca tttctcttta acatgaattc tttgtatacc tctcaaagga tagtaattcc 29160atagattctg tcctttgtcg tctcttttca tttcatcaca gttccctgaa agtattctag 29220gaaaatacta acgatgactg tcatgctaga gccactcatt ttcatcttgg tgaagatcct 29280ctctagactt acccttgaat aatctcacta cacaaagaat gtgacttagt acactgcaac 29340tcccctgcct catctgtccc agggaaaacc caatggcatc attatccctc catcatccat 29400atcaaatatg tgaaatcgtt cacttgactc tcctctatcc catacactat cccttctctt 29460ttatcatgct gaatctttct gcaactatag caacaatctc attaatgcaa agaatagaga 29520cacttaggct ttcccagttc atatggtaat aaatgtataa atttatgtct ttgcctctta 29580agatatgcaa agtaaaatct gaagtctaca agacccatca tgatctgtct ggctgcccaa 29640tcacatccag ctacttatct caacccaata cggcccctgt taaaataaaa tgctcttctt 29700ttctgcctgg taaccatcta atcctttacc acgtgtcata gtttttggat ctgaattctc 29760agtctgcttt gttgcatgat gcagatttta tgcatgtgta gtacaggtgg ggtgtagaac 29820aagtcgttca ttttaaacac gatctctgga tttttctagc agtgagacct caagctttga 29880gatttttctg ttcataaact tttttttaat tttacaaata ctaaacagca tagagctgac 29940agcagtgaaa cattaaaatg accaactctt aattgaagac gaaagtcttc ctcctgctca 30000ggtcctccta ccgcaactca aaagaccctc cctactcatt ttaacaggta ctgagggaac 30060ttctgcacta taggaactta ttacaaagat attgtcttac ttatcaagtt cttaggatta 30120atgcaagtgg tagtgaggat tggtgtatta ataactttat ttcagaattt accttcttgg 30180caatcccaac ttgcttggct gttagtttcc aatgcctcct acgctgtaag cgtctttatt 30240ggatgacact cagcttccaa ttgtatggct tactaaggaa ggaaacttct atagggcaat 30300tttcaaaaca gctgatggct aatactttat aataatgata tatgtatggc tcatgagaag 30360cacctacctg aactcaagac tggtgttaac tgcattttat tctactgtca aatggcactt 30420tagacatgtc ttaccttgga atcctttcaa gagtcaatta cctctcccat gtcatccaga 30480atctaaagac cacagaataa ctttcaaagt atattatttc aaacacacct cactggattt 30540aagaacagtt ggaaagctcc aggcatattt ggagaccagt atcagtaata ccctgataac 30600aaccagacaa agataacaag aaaactacag gccagtatct tttatgaata caggtgcaaa 30660tatgctaaac aaaatactag caaaccaaat ccagcagcat agaaaatggt ttatgtagca 30720tagacaagtg gggtaattcc aggaatgcaa ggttctttca atacaaaatt ccatcaatgt 30780aatacaacat atcaatagaa taaaggacaa aactacatga caaccttatt agatacagaa 30840gaagcatttg acaaaattca aaaccctttt cttgatttaa aaaaagactc agtaaggtag 30900gaatagatgg aatatttctc aacttgcaac atctatgaaa atcccacagc taacatcata 30960tttaatggtg aaagacctaa tgcttttcca ctacaatcag aaacaagaaa aagatgtaca 31020ctcttgccac ttcaatttaa tatcatattg gacgtgctag ccagggcact taggcaggga 31080aatcaaataa aaagaatcca gattggagag gaaacaaaag tatttatatt cagagatgca 31140caatcttgta taaaaaatta aaaacagaat cagctcagaa tagctacagt atataaggtt 31200aatacataaa aatgaattct ttctagatgt agcaatgagt aatccaaaca aatattaaaa 31260aaaaagttgt ttgtaaaagg atcaagtgaa aaacatttaa ctcaaaaaag ccaagatgta 31320tacactgaaa tctataaaat agcattgaaa gaaattacac acctaaataa atggaaagac 31380actccatatt tatggattat aaaatttaat actgttaaaa taccaacata cgtcaatctg 31440atgtatcgat tcaacacaag aacaatcaaa atcacaggtg ctttttatgt agaaattaac 31500aaatagattc taaaatccat gtggaatgca agggacccag aacaggtaaa attatcttga 31560tgtagaacaa agctagagaa ctaacacatc ctaaattcaa aacttactct agggctacag 31620taattaagac agtatggtac tggcatagga agagacacac ataggacaat gggacaaagt 31680tcaagaatcc aaaattagcc atatttatgg tcatttgtac gtgtttttga caaagatagc 31740aagcctactc ataaggaaat tactaatagt ctttgtaaca attaggtatt gttctactag 31800atatccacat attagagaat gaaactggat ccctaaaatg taccacatgc aaaaatgaat 31860caaagaccta aatttaagaa ccaaaaccat aagatactta aaagacagta ggagaaatga 31920aaacatgtac acaaagtgtt catagttgta ttcataatat gcaaaaagca gaaagaacac 31980atgtatgcac aaccaagtgg acaagcaaaa aaatacagca tatcaataca atgacaatat 32040tcagccacaa aagaaacagt accagtacat gctacaacat tgatgcagtt tgaactcatt 32100gctttgtgga aggtggcgaa acagacaaaa aggccacata attgtatgac tccattaaca 32160tgaaatgacc aaaataggca aattcatagg catacgaaga cgagtagttg ccaggggttg 32220gagttaagaa caggcagtca ctcttaatgg gtacagaaaa ctttttacag cgatgaaatg 32280ctctggaatt ttataaagac aatttcacaa ctctgtgaat atactataac cacaaatttt 32340acacttttta aaatggcgaa tttttatact atgtgaatta tatctaaaag atgtgagaaa 32400acacatctct tccatttaaa ggtggacgat ataaggtcag gttttagaaa ataagtcttc 32460attacagaca tttctaatct ctctctctct ttttttaagt taacaagcaa cgttgggaga 32520actgttctca ggatgtcttc caagatgtta tctaccagct gcatggtaat ttcactgaaa 32580ccacacaacc agagataagg taggagtggg tataatccaa gtcaagggaa tggcagtgcc 32640aaactaaaat tcagagtcat ttgactgaag gctacaaccc caaccagtgt gtactatatt 32700ccagatacat tccttatcat tttctatgtg tttctgggta atgaaaaatc ataataatga 32760tccaaggttc catgcatgtc agcataataa tcttaatttg ttgtttaatt tagtcatgtt 32820tgtttttgca aaaaggaaga tgacaaattt taggtggtca tgtggacgca accttaccat 32880ttgtcatccc ttttcctgcc tgtatgactt ttagtaaggt gtataacctc tcaagttaca 32940gatatttatt ggctttgtga aaatgaagga ccatgtgcac agatctttag cagtcattga 33000aaattcttgt cagaatttac aggaatttga gcagcaaagt ataagaaaat gttaaatatg 33060actcacatat atttacagag aacagcatct ggaggattcc tctatgaaaa tttgagagac 33120tagtcagtaa taacagaacg aattaaggta tcagttgcca tgactgctgg aagagtatga 33180gtggcataaa gacggtggtc aagagtacac cagaagacag gatgtagagt tacaaaactg 33240tcttgctttc aaccatctag tcttttccaa cttactgtct tttttaccct ggggcagata 33300taatacagtg attccagaaa ctctattcaa taaatgtatg tgtatatttc ttgattaatg 33360cttatctgcc tactgcctaa gagacagaaa gatcagtaca aggtagtttc cactgtgaat 33420ttactcagat tccatacaaa agagaagatg ctttaaaaaa aataataata atagtgggac 33480aatatcaaag ctaaaacaca ggaaagaagg aagggaacgc acttctgaag gcctcctcaa 33540ctgttcaaat aaatatgatc caccaggctg tgaccaggga tatgtaggct gctattacac 33600tatgaaggca cactgtaact aatagaagtc tttatgagta tgatactttc aaaattccaa 33660ctgaaagaat atgtgagttg tataaagtta atctaaaaag gaattttgag tagtgatgaa 33720agggatgctg aacaggatgc actagtaaat tatgcagaaa tccacctgta caaactattc 33780aaaggggaag tactcataca aatcagaaaa aaaaatgctt tttgagaatg aaaagaatta 33840gaagtagttc agagtcagga agataacctg agtaaatcaa aggattgggg cctgaaataa 33900agtacaatta aataggtctg agagacaata actgacaaca tgcatatata tgcacataaa 33960ctgtgagagg gtaggaaaca gcaaaagcta tggtaaacat ggaaaggcca ggaaacaact 34020ttgtggagtt gctctttcct tccatgttta tgtggagtct aggcagagtc ttcaggtcaa 34080gttttatata gtgagcacct ttacccacta agccatctcc ccaggccaaa aaattgacag 34140ttctaaagga agtaacaaga aataactagg agatacaaga gtaagaattt tgtttttatc 34200ggtactgttt tgtactatat aatgacttag aggtaccagg atgatgtcct ggaaacacgg 34260gtctgatatg atatgagata tgagacccac aaaatatgag gcaagagaaa aaactttggg 34320ttgaacagga agttcagagg agtacttcta agtacaatca ggagagtatc catacaaggt 34380gtctatgagt tgggatggaa aagtgagcct ttcaggaagc tgtagcccaa ctctgcaagg 34440ctctcaaggg ttaaaaacaa gacttcttta ctgtggcaaa tggagactac tagtattatt 34500gcaagtacat tttcagtgaa atattcctgg attagcagcc tttacatagt gaaatgcagt 34560acatacatgc actcaaatat tcttacaact gttaccaaag tcaattttct tatttgaaag 34620tagaagaggg gaaaaaaaaa gggcctctgt ctatattgtg taaacatttt tcctaccaaa 34680caccaggaac tgttttacac tctaaagatg aaagaagcag caaatggaag ttactattat 34740tattattatt attattattt tattctggga agggtagaaa ggattaaaat agaccctggg 34800ataagcagtt aaagaatgaa catcttaaag agtagcctca gtaaaacact tctttctcta 34860gggtatgtgg attcttgaga aaaaaattct ttaaaagagg ttgatttcag caatagccac 34920cttatggaaa cacggagtaa tgctgcctag ttgatgcatt tgccacatgt taatgtgaac 34980aagttctctg aaggctcatt tataggtttc ttttgatata tacattccta cacaggatgt 35040atttccaaat aaaggtaagg ttattgcaaa tgaatttgat cacacaatta actatagacc 35100acgatatgta cactcaattt gtttgctgca gctgccaagt ttccagagaa gttttgctgc 35160aggctgtgct tttatgccat aagtttgaaa tgttctacac tactaaaagg tattttttaa 35220ctcgtaagta ttgttccatg acaatctagt aaagcaatca tgaaaaggca gccatattaa 35280tgtggtaaca tgccgagaaa aactgattag gaagcaagtg agagtttttt tttttacctc 35340tgaaaacttt ttaggatttt tttttcactt tcttttaaat atattgatgt tacttctgtt 35400tgataatcta tattatcatt tgaaaacttt cacacagata tagtatcaca actacttcaa 35460tactgtagtg acaataaact gaagaacaca aatctcttca tgaatttatt ttataaaata 35520tacccactta atttgtcttc ttatcacttt cataatacat aagaagcaca gtggacaaga 35580tgcccattaa gatgactata gtcacattta gggggtccta taatcaaggt agcatacata 35640ccacaacagt gttctaaagg ctagctcaga aaaaaaaaaa agaaaacaaa acaaaacaaa 35700aaaacctcac gttaccttat cacaatatgg aacgcactgt gttctctttt cccattaaaa 35760tatttaagcc actgtttcat ttagcattac agtaaataag agctctgtct caactactac 35820aagaaagaaa ataatcctaa gacatccttt tccccttcca atgatttgaa agctaccatt 35880atcccctgcc aattaaaatc atcaggttga tttgcactag ttgttaaaac aatcaaggca 35940caagctcaac aggttatctg tagcttgtag taaaatgata taccagtatg tctctcctta 36000gttaaaaata cataattctt ttattttgtt atgcaacaaa agaaattaac atcacagaca 36060tagaagacta gggatgggaa actacattaa atatccagta atttaagcct atttgtactt 36120taatatagga catgaaatct ttaacacctt tgaagtaaat tttaatttgg gggatataca 36180atttatttct caggagtact agttccattc atttccaggc ctgctaactg tgtaaataag 36240taacacaagg cacagtagct gtactttctg ttatgctgca acactgatcg agtgccttga 36300aaaatggttg attaaacaag atgacggcaa cagaagggga gactttggat tgtctattaa 36360aatctcagca ataataaaga ttcattttaa gtgcactttt acatgcttat ttatacaaca 36420aacttcctaa ttttgttgca ataggcttga ctatcctatc atttggccag atgcactttc 36480cccagtaaac ttaaacaaca aaaacaacaa caacaacaaa aacaacaaca acaacaacaa 36540caacaaaaac aaaaaaaaaa tccctgtcct ttcatatact aagaggattg gctactgaaa 36600cagttcattg caagacacat gaagacaaca tactgtggca tgagttgttt taatttgttg 36660tgctgttact aaagttctga gggctgtggt tcaaacattc caagttctcc cttccatctt 36720tattggttct caaaattttg tacagaaaac tctttggggg ctagaacagc agtacttgca 36780ccacactgtt tccttgactt caagtttcaa aagcaaattg ttagaaaaaa gttcctgatt 36840tgacttagat acagggacaa aaaatagcac atacttgaag attacatgtt ctacaaatgg 36900tggcaatgtt ttccttgcga gaatagtttc gttggtatat atatcttaaa tactcaaaaa 36960ggctcaacct caagcagtaa taaacacaag caaaagtgat ttaaccctta aaataaatat 37020tcagaaaacc tctctgtaca tacaagtgaa agaatatgta acactttcac gcaaaaaaat 37080aattataata ataataaagg atttgttcat atatgcagct gaaatctgct gttccagccc 37140acaatgtccc caatgaagaa gggaggcaca gacatagatg actactgtgg ttcactatct 37200tacagccttt ttgtactggg atactattac catcaaatta tccctcttat atttattttt 37260taactttttc tttttctttt tctttttctt tttgtttaca gcatgccaaa tcctttggca 37320tatgtgatgg ccttcaacaa tctctcttta agtttttctt tgcttgaata ttccggaagt 37380aaaaggacat taaagcaagt atgagatgta ggtaacctaa ataaagagaa agggaaaaac 37440aggacaatca tgagtcattg gaaatatgct cagtgttaaa tgctcctatt agatacatat 37500cttgttagga cggtttttaa tgagaaacta cagattaaat atatcataaa cattgtactg 37560tttagttaaa aagatcagtt gggtctttat cctatgaaat aatgcaaaaa ttaaagtgat 37620gctgatgata gtaacattta ttatctattg agtttccact ctgttaccta ttgctttaga 37680tactttaaca ctgaagttca tttaaatctt tacacaacaa acatcttact aaacccatta 37740aaagctgaag tcactaaaca acttacacca aagattaaaa attattcaaa cctggctttt 37800aaagattttg aagtctatgc tctgtatata gatttgaaat actgctggca aaatgaaaat 37860gataatgatt gattataaat taattaaaat tagagccaag atgtataaaa tgtctaatta 37920acagtaataa aaatatatct gtcgatgtat atcgaagtct acctaggaca tattgttcag 37980ccccaccata aaattgtgaa gtatttattg gagaaagaat acaatcaaaa gtcagaatga 38040tttaacccta gaataagaat ctagtataat gtaaaagaac agacaatcct ctaaatttta 38100gtgtagccat tcaaagtaga ctgttctata ataaggcttt cttaataaac tatgaaacaa 38160agggggaata aaacataaca acttgataac gtctgtactt ctgtaagctc cataattact 38220ttttaactac actaattttt aaaaaaattc ctcaaatcat gaagggaaaa tatccattcc 38280actagtgttt gctgacttag atatttcaat ataacatcag aagagtatat agaggctaca 38340tgttggctct gcttgcgtgt atggaatatg gtgaaaaggt ttacagtggg tacaaagttg 38400gagtttgata ttacctttct gtgtctgggc catttttggc tataatcatc ttcaattttc 38460ctagtcctcc aacaggtgct ctgtctgtgc ctgttgtaaa ctgcagaaag agtcttttct 38520gttcatctgt aaacgaatga acaatttccc agaactccct aatgtgaaaa gagaatactg 38580gttttaattt gacatccact ataaacaaga tcatagtact acaacattaa ggccaatgtc 38640acagtgaata ttaaaagtac catttacact tagtatgtaa gttttagtca gccagatttt 38700atatcttgta ggaactatat atgggataaa tattaataac tcttttgctc agtaattcta 38760cttaaaaagt acttttgtat tcctaatgct aagcgtgatg ttttaaaatt tacttttgta 38820aaaaatacat gttcaatatc ctattcacat ttctaaaacc tcaatctaaa aatctatcaa 38880gatcttctcc aaagcaagag aaatgtgcaa taaatatatc tgtaatctat gctgagaata 38940aaattggaaa atcaagtaat aaatccccaa agtaaaagta gagagaaata atacacaaga 39000taagcacagt taaggatcag aataggacaa aaaaattgta gacactggaa gagcggaaga 39060aagaagtgac gaaactttcc aaagatgata gaaatacctg gtgaagggat tttaattttt 39120aaaaaattgt aagtgaaaag tagaaggcat tgaatcacat aaagcagaac caaatattaa 39180agaaacagca gtaaaaggtt ttccttcatt gagaaaccac ttaaagtgac tgtactatgc 39240tttgctaccc catgacattt actaagagca ggtccatggg aggaaatata taaaaaagta 39300aatgataaaa aataaattta agtccaatag gaatattatg aagagttctt caaatgagaa 39360ataaggcttt ttggaatatg aaactatttt taaaaaagac tgagtcgtga ataatataaa 39420ctctaacatg aaaagtttaa cgatggcatc atgaatcaaa aataaagaga ttaatgaatt 39480caaacagaaa taataacact tgaaaatact gaggcaggtt ggtgagatgg ctcagcaggt 39540aggagcacag actgctcttc tgaaggtcct gagttcaaag tcccagcaac ctcatggtgg 39600ctcacaacca tccgtaatga gatgatccct cttctggcga gtctgaagac agctacagtg 39660tacttatgta ttataataaa taaatctttt gggccagagt aagcagagtg accggagcga

39720gcagaggtcc taaaacttca attcccaaca accatgcgaa ggctcacaac catctgttca 39780gctacagtgt acccatatac ataaaataaa taaataattc ttaaaaagaa aaaagaaaat 39840tctgaggcaa atgcacatca actctagaaa taaaagatga ttcaaaaatg aggtaagtaa 39900atgagggaag tgtagtaatt tgtatttgac aaacatccaa catttctact tgacaacagt 39960ctagaaacaa aaagcaagca aagggttagg aagtcagaag gggaaaggag ttaaaacaaa 40020atattcaaaa ctgaagaaat gcatatcaaa gtaaaatgga aacaaattgt taattgacta 40080aaacctttca cttggtaaaa aaccctaaca agtaaagata aatgacatta atgtagagaa 40140aatactgtaa attactccag agacaaatgt ctctcatgta caagaaaagg caaagggaaa 40200ggaaatctaa aaagtattcc tatgattcag cattttgaag gttgaggcaa aaggtaggca 40260agttctaggc taggctatgc tacaagcaag ctattgttca aaagaagggt gtgaggggga 40320tcaataactg aacaataaaa tgagtaagag acaatattaa aaatatgatt aggtaatata 40380atgaccttga aaatagaaaa ctatacttca atattatgtg acagctggaa gagagcaaaa 40440aagttgcaaa gtaacctcta tgttaataag ggattatttt gataatatac ttaaaagatt 40500ctttctaggt ttgttccttg acaaggcctt accagtcata acattaaata gtaagtctta 40560ttcctggatg cagttttcta ataatttctt ttaacaatgt tttcttacag ggtaggggat 40620aatgttcaag ataaggcaaa atttcaaatt ttaacaaagt catgtgtcat gttaaagata 40680caccaacacc aagcaaagtc ctaagcaagg ctaaagtcct aatggtaaaa cacatttact 40740acattaaaag taaattcatg agttcataat atctgaaaac aaaaaaatta ccatattatc 40800tcttcagtat caatcttaaa tatatttgac tttgtctttt caatagattg atatcttaag 40860gtaacaatat aatagttgat atgaaaaaga taggaaaata ggagaatgac atatgtcaga 40920tgactagata aaattatttc cttaaatcac catttttata atcacaggtg aaatcatcaa 40980ttaaggaaag acaaaaaaga tatacaaggc aaaggactat tgatcacagc aattttgtta 41040ctgctccaca atctctgtaa acagagcaaa tttagatagg aaaatgagag gtcaacaatt 41100atgttttgtt tattttagtc ttctgaaaag gagacagaat tatttctctt tttaaaaaaa 41160tgaggaggga gttagaggaa cataaatcag caagcagtgc tgcaatggat cctgctccaa 41220gtttccttaa tgatggatgg attatgaact ggatgggtat aaaaatgtac aaagccgggc 41280tggagagatg gctcagcgat taagagcact gactacactt ccaaaggtcc tgagttcaaa 41340tcccacaaat cacacaacca tgagccacca tgtaaaggaa tgtgatgccc tcttctgatg 41400tgtctgaaga gaacataaaa atgtacaaag cctcaaatga catagagaaa gtatgaatat 41460ttcacaaaag aaatgtttct cctcataata agcaagagac tcttctaaga acaaaaagat 41520tcagtcttga aggtgactaa aatggagaaa gaaaatcaga aaaaaactca aaagaaccga 41580ggaggaatga ccatgtagaa atgagcagaa tgtgctttta caaaaaaaaa aaaaaaaaaa 41640aaagaaagaa agaaaagaaa attggacctg agcaaattaa gaatataata cacgagccag 41700gcgtggtggt gcacgccttt aatcccagca cttgggaggc agatgcaggc agatttctga 41760gttcgaggcc agcctggtct acccagcaca gccagggata cacagagaaa ccctgtcttg 41820aaaagcaaca aacaaacaaa caaacaaaag aatataatac acggactgaa gaaccattgc 41880aacaaggaca ggaatattga ctcaaggtta agaagaaaca tgacatttaa agcaatgtgt 41940acttttaaag aagctaggga agaacaatct ctaaacagag tcaactataa ctactaactc 42000cagagattac cagatggcga aaggtaaacg gaggaatctt actaacagga accaagacca 42060ctcaccatca ccagaaccca gcacacccac ttcgcccagt ccagggaacc ccaacacacc 42120tgagaaccta gacctagatt taaaagcata tctcatgatg atggtagagg acatcaagaa 42180ggactttaat aaatcactta aagaaataca ggagaacact gctaaagagt tacaagtcct 42240taaagaaaaa caggaaaaca caatcaaaca ggtagaagtc cttacagaaa aagaggaaaa 42300aacatacaaa caggtgatgg aaatgaacaa aaccatacta gacctaaaaa gggaagtaga 42360cacaataaag aaaactcaaa gcgaggcaac actagagata gaaaccctag gaaagaaatc 42420tggaaccata gatttgagca tcagcaacag aatacaagag atggaagaga gaatctcagg 42480tgcagaagat tccatagaga acattggcac aacaatcaaa gaaaatggaa aatgcaaaaa 42540gatcctaact caaaatatcc aggaaatcca ggacacaata agaagaccaa acgtacggat 42600aataggagtg gatgagaatg aagattttca actcaaaggt ccagcaaaca tcttcaacaa 42660aattattgaa gaaaacttcc caaatctaaa gaatgagatg catatgaaca tacaagaagc 42720ctacagaact ccaaatagac tggaccagaa aagaaattcc tcccgacaca taataatcag 42780aacatcaaat gcactaaata aagatagaat actaaaagca gtaagggaaa aaggtcaagt 42840aacatataaa ggcaagccta tcagaattac accagatttt tcaccagaga ctatgaaagc 42900cagaagagcc tggacagatg ttatacagac actaagagaa cacaaactgc agcccaggct 42960actataccca gccaaactct caattatcat agagggagaa accaaagtat tccacgacaa 43020aaccaaattc acgcgttatc tctccacgaa tccagccctt caaaggataa taacagaaaa 43080aaaccaatac aagaacggga acaacgccct agaaaaaaca agaaggtaat ccctcaacaa 43140acctaaaaga agacagccac aagaacagaa tgccaccttt aacaactaaa ataacaggaa 43200gcaacaatta cttttcctta atatctctta acatcaatgg tctcaactcg ccaataaaaa 43260gacatagact aacaaactgg ctacacaaac aagacccaac attttgctgc ttacaggaaa 43320ctcatctcag agaaaaagat agacactacc tcagaatgaa aggctggaaa acaattttcc 43380aagcaaatgg tatgaagaaa caagcaggag tagccatcct aatatctgat aagattgact 43440tccaacccaa agtcatcaaa aaagacaagg agggacactt cattctcatc aaaggtaaaa 43500tcctccaaga ggaactctca attctgaata tctatgctcc aaatacaaga gcagccacat 43560tcactaaaga aactttagta aagctcaaag cacacattgc acctcacaca ataatagtgg 43620gagacttcaa cacaccactt tcaccaatgg acagatcatg gaaacagaaa ctaaacaggg 43680acacactgaa actaacagaa gtgatgaaac aaatggatct gacagatatc tacagaacat 43740tttatcctaa aacaaaagga tataccttct tctcagcacc tcatggtacc ttctccaaaa 43800ttgaccacat aataggtcac aaatcaggcc tcaacagatt caaaaatatt gaaattgtcc 43860catgtatcct atcagatcac catgcactaa ggctgatctt caataacaaa ataaataaca 43920gaaagccaac attcacatgg aaactgaaca acactcttct caatgatacc ttggtcaagg 43980aaggaataaa gaaagaaatt aaagactttt tagagtttaa tgacaatgaa gccacaacgt 44040acccaaacct ttgggacaca atgaaagcat ttctaagagg gaaactcata gctatgagtg 44100ccttcaagaa aaaacgggag agagcacata ctagcagctt gacaacacat ctaaaagctc 44160tagaaaaaaa ggaagcaaat tcacccaaga ggagtagacg gcaggaaata atcaaactca 44220ggggtgaaat caaccaagtg gaaacaagaa gaactattca aagaattaac caaacgagga 44280gttggttctt tgagaaaatc aacaagatag ataaaccctt agctagactc actaaagggc 44340acagggacaa aatcctaatt aacaaaatca gaaatgaaaa gggagacata acaacagatc 44400ctgaagaaat ccaaaacacc atcagatcct tctacaaaag gctatactca acaaaactgg 44460aaaacctgga cgaaatggac aaatttctgg acagatacca ggtaccaaag ttgaatcagg 44520atcaagttga ccttctaaac agtcccatat cccctaaaga aatagaagca gttattaata 44580gtctcccagc caaaaaaagc ccaggaccag acgggtttag tgcagagttc tatcagacct 44640tcaaagaaga tctaactcca gttctgcaca aactttttca caagatagaa gtagaaggta 44700ttctacccaa ctcattttat gaagccacta ttactctgat acctaaacca cagaaagatc 44760caacaaagat agagaacttc agaccaattt ctcttatgaa catcgatgca aaaattctta 44820ataaaattct cgctaaccga atccaagaac acattaaagc aatcatccat cctgaccaag 44880taggttttat tccagggatg cagggatggt ttaatatacg aaaatccatc aatgtaatcc 44940attatataaa caaactcaaa gacaaaaacc acatgatcat ctcgttagat gcagaaaaag 45000catttgacaa gatccaacac ccattcatga taaaagttct ggaaagatca ggaattcaag 45060gccaatacct aaacatgata aaagcaatct acagcaaacc agtagccaac atcaaagtaa 45120atggagagaa gctggaagca atcccactaa aatcagggac tagacaaggc tgcccactgt 45180ctccctacct tttcaacata gtacttgaag tattagccag agcaattcga caacaaaagg 45240agatcaaggg gatacaaatt ggaaaagagg aagtcaaaat atcacttttt gcagatgata 45300tgatagtata tataagtgac cctaaaaatt ccaccagaga actcctaaac ctgataaaca 45360gcttcggtga agtagctgga tataaaatta actcaaacaa gtcaatggcc tttctctaca 45420caaagaataa acaggctgag aaagaaatta gggaaacaac acccttctca atagccacaa 45480ataatataaa atatctcggc gtgactctaa cgaaggaagt gaaagatctg tatgataaaa 45540acttcaagtc cctgaagaaa gaaattaaag aagatctcag aagatggaaa gatctcccat 45600gctcatggat tggcaggacc aacattgtaa aaatggctat ctagccaaaa gcaatctaca 45660gattcaatgc aatccccatt aaaattccaa ctcaattctt caacgaatta gaaggagcaa 45720tttgcaaatt catctggaat aacaaaaaac cgaggatagc aaaaactctt ctcaaggata 45780aaagaacctc tggtggaatc accatgcctg acctaaagct ttactacaga gcaattgtga 45840taaaaactgc atggtactgg tatagagaca gacaagtgga ccaatggaat agaattgaag 45900acccagaaat gaacccacac acctatggtc acttgatctt cgacaaggga gccaaaacca 45960tccagtggaa gaaagacagc attttcaaca attggtgctg gcacaactgg ttgttatcat 46020gtagaagaat gcgaatcgat ccatacttat ctccttgtac taaggtcaaa tctaagtgga 46080tcaaggaact tcacataaaa ccagagacac tgaaacttat agaggagaaa gtggggaaaa 46140gccttgaaga tatgggcaca ggggaaaaat tcctgaacag aacagcaatg gcttgtactg 46200taagatcgag aattgacaaa tgggacctaa tgaaactcca aagtttctgc aaggcaaaag 46260acactgtcta taagacaaaa agaccaccaa cagactggga aaggatcttt acctatccta 46320aatcagatag gggactaata tccaacatat ataaagaact caagaaggtg gacctcagaa 46380aatcaaataa cccccttaaa aaatggggct cagaactgaa caaagaattc tcacctgagg 46440aataccaaat ggcagagaag cacctgaaaa aatgttcaac atccttaatc atcagggaaa 46500tgcaaatcaa aacaaccctg agattccacc tcacaccagt gagaatggct aagatcaaaa 46560attcaggtga cagcagatgc tggcgaggat gtggagaaag aggaacactc ctccattgtt 46620ggtgggattg caggcttgta caaccactct ggaaatcagt ctggcggttc ctcagaaaat 46680tggacatagt actaccggag gatccagcaa tacctctcct gggcatatat ccagaagaag 46740ccccaactgg taagaaggac acatgctcca ctatgttcat agcagcctta tttataatag 46800ccagaaactg gaaagaaccc agatgcccct caacagagga atggatacag aaaatgtggt 46860acatctacac aatggagtac tactcagcta ttaaaaagaa tgaatttatg aaattcctag 46920ccaaatggat ggacctggag agcatcatcc tgagtgaggt aacacaatca caaaggaact 46980cacacaatat gtactcactg ataagtggat actagcccaa aacctaggat acccacgata 47040taagatacaa tttcctaaac acatgaaact caagaaaaat gaagactgaa gtgtggacac 47100tatgcccctc cttagaagtg ggaacaaaac acccatggaa ggagttacag aaacaaagta 47160tggagctgag atgaaaggat ggaccatgta gagactgcca tatccaggga tccaccccat 47220aatcagcttc caaatgctga caccattgca tacactagca agattttact gaaaggaccc 47280agatgtagct gtctcttgtg agactatgcc ggggcctagc aaacacagaa gtggatgctc 47340acagtcagct aatggatgga tcacagggct cccaatggag gagctagaga aagtacccaa 47400ggagctaaag ggatcttcaa ccctataggt ggaacaacat tatgaactaa ccagtacccc 47460tgagctcttg actctagctg catatgtatc aaaagatggc ctagtcggcc atcactggaa 47520agagaggccc attggacacg cagactttgt gtgccccggt acaggggaac gccagggcca 47580aaggggggga gtgggtgggt aggggagtgg gggtgggtgg gtaaggggga cttttggtaa 47640agcattggaa atgtaaatga gctaaatacc taataaaaaa tggaaaaaaa aaaaaaataa 47700agaagctagg acaaaaacta agacaaaaaa tagtaaaaaa gaaagaaaat gaaaaccttt 47760gaattgaaca gagaatatga agaattaatc acattaaata atggtgattt tattcacact 47820gataattaaa aaaatttttt gaaagaaaaa aaaaacaaca gttcgttgaa aaagaaatgc 47880aaaaaagaac agcaaaaagg acagctaata gaccttgaaa caaaaacaaa aaacaaacaa 47940aacaaaaaca aaaaaaaaag cggaaaactt tcatatagca atggttccag agcatgccag 48000gaaaagaaaa attaggctcc atttactgat aaaagtgaac atgaaaacct atttgaatga 48060atagttaaaa aggggatatg gcacaaagaa ctgaagaaca agagttattt ccaataaact 48120taacttgaac taaaaaagaa ctgaagtagc actacaaaag atgaggagaa agaagggcag 48180tagcattatg aattactacc ctatgaaaga tgaagagcaa accaaaccag gagctaagta 48240gttcaacaag ccattataaa aatatccacc aactattcaa gtgccccact tagatttaaa 48300gtaacaggag atttgaagcc cgaaattaca aggtgattgg tgggagaaat aatgcgggga 48360ggagaatttg gaggagattg aaagaagctt attggaattt accagaaaat ggaggaaggt 48420gtgtgtgtgt aaaccaaaca aaaacaaatg ttacctagtt ggttaatagc tcaattttta 48480ctatagaaag taagaactaa ttgatccctg atcttttaat ctattaacat gttttctccc 48540aggcatggaa caaatcattt ttctcttttg tagcacacag cagcaggtct tctttccttt 48600ctccctgtta cttaaagttt cttagtatag acatctattc tgtaaagtgg caaaaaaaaa 48660gggggggggg gaccagaggt aacacttctt tttttgcttt gttttgttct gttttcttat 48720gttggcttat ttcctcttta tagaagagga actaaagaac attcttctac aaatgaaaat 48780gataatcact atgttgcgta gaagttttgt ttgtcaagga tgcaaaattt atttctcttg 48840tcaaaaccta aaatagactg tggattttcc acgtttatag caattgaaat atatatattt 48900tgaatgaata attacttgag cttttacatc aaaaattatt ctaacataaa atacaatgta 48960tttggaactg ttccctttga atggccgatt tttaatagta gtactatgta attttgattg 49020ttatagtttc aaagtctttc tgaagaagat atagtgatga agtggtctat cattcttttc 49080atgggaaaga agcatggata agaacaacaa caaaacaaaa aaagccaagg aaagatttta 49140tttttaaaaa cacgtataat atataatact tacaaatata tgatatgacc ctgatggata 49200cattataaaa aaaactgtat ctgtactgca tgtagaagcc aagattttca acataataag 49260gcctcaagat ctaagagaat tagacatagg caaaactgac acaacttttc atttaaaatt 49320ttagaagtag ggctggaact tattcggtgg ctaaaagcac cagctactat tgcagaggat 49380ccaggctgga ttcacaaccc ctatatgatg gctcaattcc agtgtcaggg aatccaactc 49440catcctctag cctcagtgaa cactgcatac acacattgca tagactcaag tatgcaggcc 49500actcatacaa aaaaaaaata aaatcaaatc aaaaaccaaa gaattgttat ttttaaaagt 49560atttaattac ctattttcta ttgttgagtt gtttcgtatt ttaattaact gaaagaattt 49620tattttacag tataaacata tagtaatgtt attttaaaaa ataagaaaca aaaataggaa 49680tcaaaataaa acacacaatt ttattaaata gtataatcat gaaaactttg tcaatatcaa 49740caaaagatga aaactataaa gacaagaaaa ttagaaatac atagtaaaaa ctaaaattct 49800aaaataaagg ctatgaaaac aataactaga tcagaaatga agtttagaaa accttaacag 49860agaggaacct tgaaaaaatg tgacagaaat aaggttcaat tagaaataaa ttttaaaaat 49920tataacaaat acaaagaaac tagagataaa ttcaaaacta tacacaacta ggaatataga 49980caattttaca atcttttaca aacctaaaat agaaagatta aaactatgaa gattcaaaaa 50040gcaattaagt tgcaccatgg ttcaaatata cagtattaat gggctatggg caaagttctg 50100ctaggaatag ttgttgccct gaagtgctct cctatccttt gagtcttaga gaccctaggt 50160aagacaaaga taaccacata ggctgaggta ctgttagaag taccagcaaa aagaagctgg 50220caagaagcta catgaagtat atattcgcca acatgcaaat agctgaggaa tagttttcct 50280ctactcaaag acaggaagga gggagggagg gagggaggga gggagggagg gggagagaga 50340gagagagaga gagagagaga gagagagaga gagagagaga gagtgagtct cactggagaa 50400acatgccatt cttatgtgtg gggcccatat ccaacagtta gttgaccaac acaatggcat 50460ctgcagaggt tcttggtctc ataatgttaa ttcatggtgt ttctcccccc catatcttaa 50520aggtcctctg attatgtatt agagcttcta gttttgtgag ttttgcattt tttatgagat 50580tctctagtac acaaatatgt gtgtctctac atctatagaa tcatctagaa atttttctct 50640ttggttcttt ttcttgcttg gctgttgcgt tatcctaatt tgtttgttgt ttgtttatct 50700ttattattcc ttgatgcttg tttgcttcct tataagagtc agaaaaggca gggagcagat 50760gggaggggag gtgtaggcaa tctggaaggg gtaggaggac agggaaaaca ataaatatat 50820tatatggaaa aacaatccat ttttaagaaa gccctcaaaa aataaaacgg aaactatcat 50880gccaagaaca aagaaattaa aacaactcat gccaacagaa attaaaaaat tgtttgaatt 50940ttactgacaa ggcttttaaa gcaatgaaag taattataag cttattctca gaaatgaaca 51000atgaaataat gcataagaaa catattattt aaacttattc actaaagcat acattatttc 51060aacacaaaaa ttacaaatca atgctggatg aaactatggt ataactaaat ggaaagtgct 51120atgccatgct tgagatttag aaaatccaaa gtgatgaaaa tgacaattct caaattgatt 51180tatccaatca attcaaccca aatcaaaaca tcaaaaataa atgttattca tgtaaaattc 51240agcaaacaga ttttaatatt tacatggaag taaaattagt gaaaacatta agaacaatga 51300aaaagtaaag ggattctcac catctgatat aaagacttgc tctttgttct atcaaggtaa 51360aggagtcatc aagatgtgat tttgatcaaa gaacaaatga ataagcagag aagaaccaag 51420tctagaagta actcacacaa aactcaattt caacaaaaat aaaattcagt gggggaaaga 51480aagctgcaga ctagaataac acatgtataa catgtttgca tatgatcctt taaaaagaat 51540acattccaaa agaaatagtt cataatcagt ggaaagaaga gtggggaaga taattcacaa 51600agtaatggtt aaaaggatgg accatctagt gattgccata tctggggatc caccccataa 51660tcagcttcca aacgctgaca ccactgcata cactagcaag attttgctga aaggacccag 51720atgtagctgt ctcttgtgag actatgctgg ggcttagcaa acacagaagt ggatgctcac 51780agtcagctat tggatggatc acagggctcc caatggagga gctagagaaa gtacccaagg 51840agctaaaggg atctgcaacc ctataggtgg aacaacatta tgaactaacc agtaccccgg 51900agttcttgtc tctagctgca tatgtatcaa aagatggcct agtcggccat cactgcaaag 51960agaggcccat tggacacgca aactgtatat gccccagtac aggggaacgc cagggccaaa 52020aaaatgagaa tgggtgggta gggaagttga gggggggagg gtatggggga cttttgggat 52080agcattggaa atgtaattga agaaaatatg taataaaaat attttaaaaa aagataaata 52140cctaaaagac agctcatgat cctgagtcat cagtgataag caaatttata ctaaggtaaa 52200attttatttc acactccaag acaacatgac caaagattaa aagagataca gaatacgttc 52260tagcactgct tgtcatctta ggtaaaatgg cagtatcatt ttacaaaagc attcttgcag 52320tgcttcttaa ggttaaaaat gttatttact tcacaaccac aatcctccaa agtgcttctt 52380caggagaaat gaaaacactt caacactaag aatgaaaact ttatttgtaa cagccccact 52440gtcatgataa ttctattgcc aataacaaaa ggataataat caaaatgcat acattaatca 52500taatcaatct aaaacattat tttgccatat tgtagagata atactaggat attcccatta 52560aggcttctgt attaccatgt ttatccctgt tatgaattac ctttaaaact gagcagcaat 52620ataatggttg ccacttttgt gactggtgtt agaagcaaat atgaatgata aatttttata 52680ttcattaata attgccattg tgcttagaaa tgatatatca tatgacatgt tatggttttt 52740gttttgcttt actctgaatt ttgttattta attacctaaa agctgaataa ataacatatt 52800tgcttttcta ttaaaaaaaa aagggcaaag caagagtaat cttctacaaa cttaaatgaa 52860taaagtaata ctgtaatata aatataacaa tgataatgta tcattactag acacagttaa 52920tattcattct attgttaaca tatttgagtc ttatagctca agtgatctac aaaaagataa 52980ctgtttaaat acctatgaca tcatagtgga tgccaaaaag actactctgc attttgatca 53040gtcacattag aagatggggg gaaagatctt tcagaattgg atagtagcat attacagaag 53100tagaaataag ggcatttgac tagagaacga tgttagtggt aatactgtcc taagaattgt 53160tcagaatcac tagtatacat ggttcttccc aagtatctta aagactaaat aatgtgccat 53220cttacacatg accaaaaata aggcaaaggg ttgagaaaat atgagactta aatcatttaa 53280ttcatgaaat aaagcattag actgcatgaa aattggaaat attaatacag gtctattttt 53340gagcttactt gtttggtaac cagtacctac agatacagga cacaaaagca cacatctaca 53400tgtttgatga ataaaatctt cctttttaag aattaaacac cttacctaat cacaacagat 53460tccctcgtat agccaccgtc atactctgta gtttcttcta gtgcctggaa atctagattc 53520tgaaaattat agggggaaaa aaaagcacct taacatccat agtacaccaa aacaaaaaac 53580aaaacaaaac acccaaaaca aaaaaaaaaa caaacaaaaa caaacaaaca aaaaaaagac 53640cttttttgta tatgtctttc ttacccggct tccacatata agcaattcaa tttcttctgg 53700tctgaataag tattttaagg gcgattcatt agtcaccata tgaaaacctc tgcgaaatgc 53760cttgaattgt ttttctacag atttattgag aatgtagtct gaatagagat tgacaaattc 53820ctgtaggaat attaacaaga aagcatattc tatacaaatg aaacacaatt agcttttgga 53880ctagttttaa tacccaccct ccaccccaag aaaaccttca gcagtatttt ttttgttact 53940atgtttaaaa attagatttc agagagttgg ttcagtgctt aaaaaagcat tagcaactct 54000tgaagaataa acataagtct taaaaaatta atcacatgtt atatgtacaa agaaaattat 54060tttcacaatt tctcacttct gacagatata aaagttgtat tcatatgcat agttatacat 54120tatgattcta tgagtaaatc aaagagaaat tatataaaat gtaattaata gtaataataa 54180tgaaataatg taaacagtaa gattaaaata aaagatttaa aaagactgta tatatatata 54240acaacagaac ctctagtatg caatataaat ttgttatgaa tactgaactt gagaaaacca 54300gaatgtctca ggtttcctct ggaaattatg gtactggtaa gttagctact gtatccagct 54360cagcaaaaat gttatttgtt ggtaaactaa gctattataa caaattataa aattttatag 54420tttttataac tacttcaaac catactaggt aattctttga aaactttaca aattataaaa 54480tttgttatag tacaaatgta gtttgtatca tgacagtaca atatttcacc aaaaataata 54540gtataaatta atatatctta aaattatact atgtaatatt ttcttttgaa gctaagttat 54600ttcctattgc caagtaaata gcttttaaag ttagtaaaaa ccaaatctaa tgtgaataat 54660ctgatatgca taattagatc tcatgacagt acaatatttc accaaaaata agtataaatt 54720aatatatttc ttaaaattat actatgtaat attttctttt taagctaagt tatttcctat

54780tgccaagtaa atagctttta aagttagtaa aaaccaaatc taatgtgaat aatctgatat 54840gcataattag atctcaaatg gaaaatgtat atacacacta aaccagatgt caatcactgg 54900gctctgtata cctcttaggt ttaatatggt aaggccctgt ttctagcgtt acattatagt 54960aatatttacc cattcaatct tttgtagtct gtttagtctt tctctaaagg ggaattaaca 55020attctgtaca atcacataag ctcttatcta agaggaaagt gtagctttac tatttagata 55080aatttgttta tgtaaagttt ttataactgc ttcaaaccat actaggtaat tctttgaaaa 55140ctttactgct ttgaaattat atatttataa aacaatgaca aaaatttaat taccttcctg 55200ttttcatttg taattggaat tttatcacca ttttctttta gatcatacat cattgggtta 55260ccaaaaagat ctgtctgtga tatctggaaa gtgatcatca tatcatcttc cacactccct 55320tcatattcca ataaatcctt taaactctga tataaaacct aacaaccaga aagagaagtg 55380aggttgccaa ataaatacta aaagaaataa aaataataga ttgttcattc tttgtagaaa 55440caacttgtca acaaaaatga agccataaat attccacatc tctcaagtag tttaataaga 55500tagaaaggtt gtcacaggca atgggtcaat cttaaaaatt cttcaaatga tcagttcagc 55560aacagatttc ataccatggt ctagaatata cactttctct ccatgattgt ggcagggtac 55620aggaaatgag gaaaaacaaa caaacaaaca aacaaacaaa caggaaaaca aacaacaaaa 55680aagccacaca catatcaact gtacctatca acaactttac tacatagact catatgggac 55740attgaaaaca tgatgttttc aacatttttt atggggtcaa tcaaaacata cttgatagga 55800tatataaaaa atgatgttta ttatacttaa ccagattaaa agtattttac tttactaaac 55860agcagtatca acaaaagttt acaaataaat aatgttccta tataaactga gataaacttc 55920tgtatttaga tacaatctta aagactatta acgaaggaca acttactggg tgagagtctc 55980ccaagtcacg aaaggttcct tttttcccca ttagcttcct gtatacaacc atgggaaaat 56040ggacatccag tatacaatta ttgtaaatag ccagacccag gactatgcca atcagagtaa 56100actgaccctc agtttcaaaa gaagatggat taaaccaaaa taatttcgta gcttcatcat 56160atgtgaacat acctataaag aaataattaa aagtcatttt atccacttaa tgactgaaaa 56220ttacatatga aacaaatgca tgattaagta aaaaaaaaaa aaacaattgg agaaagttga 56280ttaactttaa gttacttaaa attcatatca aatctaagct aatataattt taaaaaatat 56340tttgtgatct ttcagcttca catgatcttc attattcaaa gccaaagtcc agagattttt 56400tttcctatca tcattatttt atctaggtga acattaagag atagtctttt ttttcttttt 56460ttacggaaca tcaaacaata aaataaaaca aaactaattg tatatgcctt tcaatttttc 56520taagtttatt ttcagttgct tctcaggaaa tactttttat acaaagtcta acaagctggg 56580ggagagagtg ggctgtatac cttagcactt ggtaaaatga gtaaggaaaa ttatgagctt 56640aaggttagag tactaataca atataaaaag attgtctaaa aaagccaaaa tcaaaatgag 56700caaatcaaaa agttttggga aatgtttttc taatggaaca agagtattaa aaaacaaata 56760ataaagcatt aaaatgtact ttcaggttaa ttttaatatt taaaatgttt ccttattttc 56820actttcagtt ttaattaaaa agaaaagtac acttttctcc ttgaactgta gtaatataaa 56880ttaagtctaa gctttttctt gcaatttaat atttgtggaa tactggtttc acaagtattc 56940aattcatact tgcattccag cttgaaaaat gtgagttatg tatgtctgta tcatttgcat 57000gccaggttat acatccaggt aatatcaaaa gttccagttc acaaataata aatctgatag 57060aatgtcacca atggatgctc ttggtctcaa ggacaattaa gctataaatt aaccagagga 57120taaattatat aacctatgat tctcatgact tcaggttttc aaatctacca ctctaaggtt 57180tttcaaatta aatttcccta gaaggcaact gtgttctact agaatatttg actaagtaat 57240atatggaaga acctaaatta agtgagtgat agaacagctc ctgaagcagg gatatggcat 57300actcaatttt tttactgctt accataactg aaaatattta taaaccaagt ttatacttta 57360aaagactaac acaccatcat aatcatatca ctaatactta ccaatatctg gattaaaaat 57420ttcctccaca accaactgaa aaaactcttt ggaaacgcct ccctcatcta ctccttgttc 57480tccttcaaat tccacataca actgcttctt caagtctgca ggattttcca tagcaatcat 57540ctctagctgt tgattgaaaa tataaaaaag aggacagttg agaagaggaa aaatgtaaga 57600tataagtaga agaaaataag aagcagcaat aacaaaacaa tatggaaaga aaacaatgaa 57660ttttgtttct gaaaagaaaa aaagcaaact gacttacagt ttttggcaag atttatttca 57720acacacaaga gtatgtactg tgtttaagaa gctatgcatt tcttagtaaa ggatatagcc 57780aaaatgctgt tgggggtggg gtggggggaa tagttgcaat tatgtcctgt atatcttcat 57840ctttaggggt ctaagggcct atgaatttat tctgtaaaga atctctatat tggtctactt 57900taactaattt gtatgtcttc atgtatgtat attcacatgt ctccttccac ctaagatgta 57960tttcctcagg atctgtccac cttgtttgtc aggaaaggat ctctccttga cctggaagac 58020tgcataacac aataggttgg actgtcaatg gcccctggca tctctgccag tacctgccta 58080cctagcatca ggactacaat gcccacttca agtttctttt ctttaataga gttctgaaga 58140ttaaactcag ttcttcttgc aagttgatct accttctcag ctcgcatacc caattttcag 58200gtcagtattc attgaaattt attttcagca gtactttgtt ggtgaacata taagaaactg 58260ttctttcaca agttccacat atcaccacta aatattaaca tttaattact aagcttcaat 58320aaatcaatcc tattttctaa tgtattacag aaacagaggt aatattcttc ctactctaat 58380ggtatatgct taatatatca tttctaagga gcaaaactgt taacatatta cccttcaaaa 58440ctatagactt ttaaaattca taaatttatg tccccaagaa attaataggt aggtctgaca 58500ctttacagcc aaatatatag atttgaattt aaccttagaa atggagaaca aaattatacc 58560aacaacagca ttagctagaa acctgttcta agacagaata actttgtaca cttaaatttc 58620catgatcatc ttagctcaaa tttggtccca acacaatata ctattattac tgtggtttct 58680cccagttctc aacttgctaa gaaaacatgt taattattga ttcaaaggct tgttggtttc 58740aagacagcat acatctgtcc agtctctacc cttttccccc aattctggtt ctagtatgtt 58800ctattatgat cactaaaaat aattttctat atttttgctt tcgtttgaaa tgtaaaataa 58860gcataatgag atagtatcaa ataaataagt gaagtgtgta aattaatgct ctaccattaa 58920ctctaagttt gaagttgcaa gtattgtcat tctagtaatt tttaaatatt ccatgttttc 58980tatatatgat agaacaagca tttcatccca gcactcaaac tcggtaagtt tagtgcagtt 59040atgtctacac agaaaatctg agtccagtga gagctttata gtgattctct atctaaaata 59100tgataacaag taaataagta tttccagttt tgctagaata tttaaccaca tgattttaaa 59160aacagaagta ttcccggaat aagagcagca gcagcaacaa attatgaaac ttacagctca 59220ctttacaatt aaaattgttc tgatatctac aactccttag caagtaggct gctaaaacca 59280ttttacagaa agtcccttga agtaataaaa ggataaataa cttaagaaat agtaattcag 59340aagagaagtt ttaatccagg gagcaagctt atagcccaca gtttacctac ttttccaaag 59400aaaatagaga taatatttgc ttttagattt tgtgtgactt ctttggatat caatattttc 59460aactatattc acatattatt ttcagaatca ggatatattt gtatataatt taaaaatagt 59520tggcagctta attctgtaag tgaaaatgtc caactagcaa tttaagaaga ccattctcaa 59580tgttgccaaa gtgactttgt aaattcttat tttgtttgag atgatgcatc agtagcctgt 59640ctttttaaaa actatggtaa caaaacaaaa cataaaattt aatactaacc atacacaaaa 59700gccattagca aagaaagtca cattattttt ttttccttaa atatagtaac tcttgctgag 59760aggtggtagc acaagccttt aaactcagca ctcaagaggc agaggcaggt ggatctctga 59820gatctagacc agactagtca acagagcgag caagttccag gacagccagg gctacataaa 59880ccctctcaaa atacaacccg cccaaaaatg taactcttag aagagacaca cacacaccct 59940taaaccattg tttcatcaac taaagaatag tataaaaaaa aaaattaaag ttatatttta 60000aaaatgaatt tgtaaaatca agctgtctta catgtaattg gatacatgaa aactattggg 60060atatacttaa aattctgaaa aaaatataag acaaaatagg tagtattggc ttcatagata 60120gcgctggaga aaaaatacac tggtattact atgctgtatt aatgtacaat agtaactatc 60180acttcctaac tgtacttctc ttcatactag gaaatctcac tctcatcaac tgttaaaata 60240ttattcttaa tttatttggc aatacaaaat cctagagaga tcatttttta caagctgacc 60300ttataggaga gcattccaag aaatctaagc agaatgatat actgatttaa aaaaaaaaaa 60360aaacattcta taaccatatc ttcaaggatc aaaaaaaaat ttagcgccat catttaaaac 60420ctttagacag ggctggaggg atgatgtatc agcagttaag agcactgact gctctttcaa 60480gtcctaagtt caattccaag caaacacttg gtggctcaaa gctatctgta ataggatctg 60540atgctccctt ctgatgtgtc tgaaaataaa taaacatgaa aggaaggaaa caaggaaaga 60600aggcgggcag gcaggcaggc aggcaggcag gcaggcaggc taaataaaac ctttagacat 60660ccagagttac catctaaaat cctatgttaa cataactgaa aacaaaaaac tactaatgat 60720actatttaag ttgaacaaca ctcaaattag ctctatttaa aaatgtttag actctccaag 60780aagagtaatt tatacacttt gataaaaatc taaatatgac tttcatttct ataagtaaaa 60840atctctctaa atttctttct tttaaattat tatatgatgc taagctctta tcgtactgac 60900tacttcatct cccattctgg ttgtaagaaa ttattttgaa gataaaggga agaggattcc 60960attaatttct tttttttttc tttctttctt tcttcttctt cttttttttt tttttttttt 61020tttttttttt tttttttaca cgagacaggg tttctctgtg tagcccaggc tatcctgaaa 61080ctcactctgt agctggcctc gaactcagaa atctgcctgc ctctgcctcc cgagtgctgg 61140gattaaaggc ttgcgccacc acgcccggct tccattaatt tctattagaa taattttaaa 61200taagagaaat cacattaagt acaattctat taatgaatga attacaccta gagcagcaca 61260gaaaaactcc attattaaag caatgtatgt caaatgattt ttttaaatca agttcttgtt 61320gcaataggta tttatactaa gagaaatgat ggaagctcag ctagacaatg catctagact 61380ttcagagaaa ttgaggaagt aattcaaaca actttttctt catcgatgtg tgccagctcc 61440tgggtttttg gacttttctt tccaattcta caacttagca attatttttg gttaactatg 61500gaaaattcta gaacttaact aaatttattc atgtcccata ccactgtcaa ctcctatttt 61560agtcacactg acttcattat tcgttttaaa cactggcaat attctacaca tggatattat 61620tcaataattt atacttataa actgtactaa ctgttgtttc ccaaatattg atttgtagca 61680tagatgtacc aaaacatgta aagaacagac cactacattt tcatatcaga attctttttt 61740tttttttttc ctatcagaat tcttagtctg aactcctaaa agtccagacc ttaaattctg 61800ttgctgtgtt tttattttct gggcaattct gcttttagat tgtgaacgca gtctaaagca 61860tctcgcacat ggcaatactg tctctggata catctgatct tattccactt tttaaaaaaa 61920ggttttaaga ttttatatat gctatatcct tgaaatcact ttatttttta aaaagaggag 61980aaaaatgtgc catttatttg ctggtggtag aagaaaacat gtgccaagga gttaattacg 62040tcttgtggat taaactcagt catcaagcct ggtggcaagt gactttaccc aataaaccaa 62100cacaacagcc tacaaagaat atctgttttt tgtttttttt tttttgtttt atttgttttt 62160tttttttttt aagactgtta aaacttctac actaggattg ttgttcaaat ggaagctggg 62220atctcattag tatttatatc tgtataattt tctcgttaaa aaaaaaaggc caaaaccatt 62280caaaataaag caaaagaaat attggagttt tttccttttt agtagaattt gtagatttat 62340ttctttatta tttatttatt gcttagtaca tacaaatttt aaaaaatagt accttatact 62400tttatactga ctattctctt gtgagacaga tcttctaaaa ataacagttg ggggaaagag 62460ttgttatact gattgaaaat aatgaataaa cttttgctta caaaaaagaa ttggctagga 62520gtgttataca cacataattt tagcactcaa gtggaaaaga taaacaagat cagaagtcca 62580aggataggct aaacattctt tattaaataa aaataaaaag tgatttccag gacagccagg 62640gctacacaga gaaaccctgt ctcgaaaaac aaaaaacaaa aacaaacaaa caaacaaaat 62700taattctaat ttactcgcct actgtctacc attaagccag ctaatctaat taacactgaa 62760atgtgagatt caatagaaaa cttacaaaag tcacatatgt aatcttttct aggcctcact 62820tataaactaa ggagaaaacg gtattatttc cttcatagct caaggttagg gttctataac 62880ctttctttct ttctctctct ctctctctct ctctctctct ctctctcttt ctttctttct 62940ttctttcttt ctttctttat atttattaat gttgagttca ctgcaaaact tctcattcct 63000tctctataca accttactat gcagttcaaa atgaccgggt cataaaagat tgtgattaac 63060atgaatattc tgccaagttt ttttatgaaa gaaagaacaa ggtttcaaaa caatagggat 63120ttgtggagaa taaagacgtg gaaagagagt agaattccat cagttcatat gtgggtattc 63180ttttgaatac agcttgctgt aaacctccac ctttttgcta ttaatctaat atgaatataa 63240taattatcct ttgctcaggg tcatcaattt gatctctaaa gtctgagtct ttgcttctat 63300ttctaaccct ttcccactag tatgctgaaa aaccaagaaa ttttagaggg gttcagttga 63360aaatgatcca gaaagagaaa aaaagaggag aagtggaaaa gaaagcaata taggacaaag 63420aagagctggg tgaaactctt aggaattttc tcaaaaagta cctatctttg taattctact 63480gcttatcaat gagacaaaca cacagaattg atgaaggcca gggcttctat aaaatactac 63540tagtcgaata tggatattat caaatgaatc atgtcaaacc atatgactag ttcacataaa 63600taagatttac tagcaacagt ttgttagcat aattacaaga ctcaactgtt aattttactc 63660caactacaga aggggctaat ttcgtcctaa atgaaatttt acaaatacta tccaagtttt 63720aactggttct taactcaata actttattat agaaaaaaat agtataagaa gataataaaa 63780aaaaaatcag gtaggacagg tgcataaatt ttttattaaa tgtgtattta gctcagtctt 63840aaggcttttc agatttcgac aatccattcc cccccaaaaa aaataaatgt tcaaataaat 63900attatttgtt agttatctgt taatgagtta atattatttg ttagactata aggatgatgc 63960ttcaatctgt aactagtact aaatcttaca tcaagtttat acaagaacta gcactaacag 64020gaacaattaa taccaaggga taccctgaat tacttatata taattttgtt aatatgtgac 64080tgacaatttt caaaccaaat ttctggttta actgacaggg actctgtaat ttttgtcgtc 64140tgtagaaaaa aggaagacaa gaggcaatgt tatttgaaaa caaaaacaaa aatcacgcag 64200taagaagttc ctgacttccc agttaaatca tctagattta cctggtatta ctaacattaa 64260ttccaagctc ccaaaatagc cactttacag aattttcaag attcatgtta ttattattta 64320aaaaaacaaa atcctacaat gtcacacaag acagagtggc ttagaactcc aaagtcttat 64380tgataataca gactaacctt tcctggtcat atcatcaagt tcacaataga aagctgagtt 64440gtgttacaca taatggaatt tgaagagcag ctcttattct tttacaatgt acatttattg 64500cattatttct tccaattcca tcttccattc ttaaaaatgt gtataacaat accatttgtc 64560atgtgttcca atctttgaaa accaatcttt tcttcttaat ggaaatatat gaggctggct 64620ttcaagattc agaaaatcca ttccttttac ttgctttgtg cctgtctgtc tgcgcatctg 64680tgtgcatatg tatgcatgca tgcatgcatg catgtgtgca tgtaaatgcc aaaaggggaa 64740aacgtatgtg tggaggtcaa agaacatcaa gtatcagact ttgctttcta ccaactgaga 64800cagggtctag ttatttgtca gtttatatat cagattagct tctgaggatt tttctgtcta 64860tactttctat cttgctttag gcagtactat tacaaatgga actaccatat cctgttttac 64920atgtattcag aaaatccaag gataagtcct catgtttgca caacaaacgt tttaaccact 64980gagtcattcc cctagtacta gaaaaatcta ttcttcttca aaagagaatt atcattagac 65040aaccaggata ccagtatttt tggtaagaag caacaaaggt gtagcaatac agatcagaaa 65100tgttaacata atcctatttt tttccaaaat tatagataaa atgaataaat tgaaatacat 65160cactgtctag gttttagttt aattccaatt ttaaataata gtcaaactta cccggaccag 65220tgcatcatct ataatatggt cacgtctgac tttgagtctc aaatacggat tcaactgctg 65280tccttgaact aggctgtaaa gaacagtgat tcttctttca ctgtacatgc gaattctatt 65340gtcataatat aatcccagat tctttgtgac agcattcaat ataaagggac atgtcataaa 65400agagaatttg ttctctgttt caactttgaa aaaggtataa tctttgtcca tttctagaac 65460atcattcagt ggttcattaa tgaattcttc aaaggagata agtggttttc gacagtctag 65520agttttaacg ccaagttcgg tttctagtgg atccactcga ggacctttct tatttcttct 65580ttcatctccc agaagctcct gaagtgttaa ttcgctggac tcaggtatgg gttcttcatc 65640atcttcctca ttatgatttg tgtccacatc ccctcccact acatttgcat agtaaaccat 65700tttcaaacac tttgaagcag caacaatggc atcatcatca ttcactagat ttcggctatt 65760aaattcattg cttatgactt tgtaggtaat aagttgctga aatgtttcca tcattctccg 65820aatctggtca gcactgtatt tagaccacag cctaatcagt tttccttgag cttcaagggg 65880tagcttacac atagctttgc aaaataatgg caacgccatt tccagatatt caggactgtg 65940gagattacta ttctccatta caataatgaa caaattgaga taattaggat ctcgagtata 66000cacattatga tatgtcaaat cacattccac gttaggtgac agatatacaa gtgcattcag 66060gaaggcagtt tctaattttt cattagcgag caaactgctg tagacccttc taatagcatc 66120aatatccaca gtcacatcat caggacctaa tttttgtaca ttgttgtctc cctgtgaact 66180atcacccatc cttgaagaag atgcttctga gtcttcttcc atagcagcag cagaacatgc 66240agctttttcc ttttcatctt catccttgtc ttcatccttt tcttgaagag atttcaattc 66300ctcctttgtg tgctgtttga ctttccgaaa gctcagaacc agtgcctcag cactagaaaa 66360tattcttcca attacacgaa ttaaagggga ataatcctca ctctctctac aaaattcata 66420aatttcatat actttctctt cagttaggta aatcacatct agaaaatgga aagaaaaaga 66480acatttatgt tgtaatatac atgtttattt tgttaaaata attgctagtg aaaaagtaaa 66540atatctaaac attctggact tgatttagaa tttttaaatg atgtaattta cttattaaca 66600agtaatccca acaatctcac cctttcttgg cattcattga acaaaatata attattaatt 66660atgcattgat aattaagaca tgccttgtaa ccaagacatg tactggtatg taacattaat 66720tgtaggtatg aacaaatata tatacttgtc atttcctcat tgtactttat tacaacctta 66780acaaaaagct actatataaa atcctgaaat cattttcctt tgcattgttc tcaaaaaact 66840aaggaggtaa ggttttataa caaggtttcc ttagcccctt catttgcaat caactctttc 66900tatacttttg tatatactaa aaccaaactg actcatctta tgtattccta atttcttcca 66960gaatttccat tcagaggctg aggaaaagat attactactc atgtaaactc tactgaagtt 67020aaaactgcta ctacaactac tactacctat gaccattact caggttctaa caattcttaa 67080cagagcaact ggaaattatt acagtcatca gtcatttaaa aacaaacaga agaaagaagc 67140taagaaagca aaaaccacaa gcactaaaac ctatatttat tttctcccag aaatagaatt 67200gggatcataa aagtctctag cttctcacca ctaggccaaa tgtaaatttc aacaaaggct 67260ttaccatttc aggttaacaa aacaaaagat acttatcttt ttggaaaaag gtaacatttt 67320acatattaaa gttttagaaa gtaatatatt tatcaaataa taatgttgaa accactaatc 67380tctttcacca aagagtaaca tattataatt attaaacatt taagttagga taattttatg 67440gttacattga taatacacct catacattgc caatatatct aacatgtaaa atctttatta 67500tgattgctac atattattaa aggtacctaa ttcaaaaata agaaacaata taaaattcaa 67560gtcctcattg atggaatggc ttccaaaaca taaaccacaa agcactaaac tatatatact 67620acaaacctgt ctatggccca ttctagagta ttgatacttt ttaattttat gatcttcaat 67680tctttctttt aaaaaaatgt tctatttact tattagaggt gatgagggta ttgagctgag 67740ttgataacct aaaagaatta attttctcct tctacagtgt gagtttcagt agttacatgt 67800ccctatctga ggcagcatgg caagccaagt tcaattctta gtctactaag aataagtaaa 67860aaagaaaaca ctaacatgga gctcatttct cgttattcta actgaaaaaa atacatggaa 67920tcttctttaa ccattcaagt ctaccaaatt tctctaaact gtaaccaaaa cacagaaaat 67980atcagtaatg aataaatagg cagactgtta gaatataatg ttttatctcc ctatcattga 68040attataaaat tagttagaaa gtgcaaattc tacatacaga acaatataca tcctgcctga 68100aagagaataa agctctagat ccacacacct ttgatttgca atgtcttcct ttcagagcac 68160agtcattaaa tcactctgac attctagtaa tacaaactac tattcattga tatttgttcc 68220acgtagtact ctacttaaaa tgtttaaaat gtttatctcc atctccccca cccctctcat 68280gtgtgcatat aaatcactgc aatcagaaat aaaaagttct aatctgggta tcaacaaaca 68340gttaagtaaa gaaacttttt ttttttaaga ttcacctgta cattctaata tatgtgtgct 68400tgaaaactgt ttgagacatg gtttcacata gtagaaacag aactggaaat cagctcaaaa 68460tctttaaaac ataacatttt atactccaaa cagtttcacg caaaaacaga gttgtgatac 68520actagctgat aactacacct ctaactaaag tgaacaaata taattatcaa actgagggaa 68580acatgattaa gcatatataa ttcaaaaata ttaaagaagt aaaggcaata aaatttggag 68640tgatccacaa aaacaaagaa cctgtaaaaa aggaacaccc atgggctaaa acaaggcaac 68700tatatccatt atatactaga tttttcccac agttagttgc attcaactat gatgggatac 68760tatttttcat ttactgttac tctgtcatgt ccactctgtt gtagaaacat gtataattga 68820gccaatgcaa ttttcacatt caaaatattg attagcattc acctacttat ctacaatgta 68880aacaaattca tgaaatagga ctggctatat atttttgagt agcaaagctg aaagtttttt 68940tttctgattt ttctttagtc ctattgtgaa agtaatatca tgaagttgta ctataatttt 69000atttcactaa ttcatttaaa catgtttcaa gtgatagtgg aactaatccc tggcaatctg 69060aaacattcaa ttacttccat taagagaaaa aaatagattg aacaaaatca atcatgaggc 69120aaagtaggaa atttgaagaa ttttcaaaat atagaactat ttttaaaata ctagatgcat 69180tggggacata gcttaatcgt gagtgcttat ttttcactca tgaggctatc ctcagtacca 69240ggtatcagag tataaaactt taatttcatt actggggaaa tagaagtgtg agtattaaaa 69300gtacaagaat agtgccactt aacttgcaca aaacaaaatg aaacgaaacc ccacaagagc 69360tgagtaaaga ctattactgg taagttacgt ccctaagcag tatgaaaata tctaattcct 69420gacgtagaga gggcagaatg taagaaggaa aaaatgtaat tttcagataa ctattgcaaa 69480attttaccct gctttcttaa aaaaagaaaa gaaaaaaaaa aaaagactac tttacacttt 69540atatttgtat ttctcctttt accacagtcc ctggagttct agagccgata ctaggtattc 69600acggcaaatg caatgtacat agtagatttt tgtgacctaa taaggtatta gctacaatat 69660aaatcattac aaactgagtt caactcatca tcatattaat ttttagagta taacttgctt 69720ctgactgaca actaatgaat cagtaaaata taagaataaa atgtaaacaa actaaatacc 69780agtcctttac cacagtttac cagaaaaggt gacttttact atattatagt gcgataactt

69840aaggtgataa aaaaagcaaa aacaaaaata aaaacaaaat aacaacaaca aacaaaaaaa 69900caaaatcaaa acaaataaac aaccccctcc acatttaaat ctgatatagc tttcaattaa 69960ccattttgaa ttgaaatata atctcttacc tttaaaatct tttccttcct tcttgttcat 70020ttttatctct gagttgttag atgcaccttt tgagttctca aggtaagctg agcttgctcc 70080tttcttggag ggatgaggat cacagagttt tgcattaatt ttataaagct caagggcttt 70140aatagctgct gcattgttat ccatacgaag aaaagttgga caggaagcac aaaactcatt 70200cgtgcaggcc tcatttccac agccctcagt taactgatgg tagtagcgtt ctattagatg 70260ctttgcagct gctcgcttcc tgtaccagac attcaaacaa taagcacatt tattagtata 70320gctcagaaac aaagcccaac agataaggca aaagttagct aagcaataaa gcaataaact 70380aaatatttag atgtagaaac tggaaattca ctactacctc ccaaagtgtt agtaaaataa 70440taaacatttt ctaaaaaaac tgtgataaac tatgtcagct tataagataa ttttaatatg 70500gcaggctttt ctttggttta aaaaaagata agcttaagtt gatggtatag gacccttaac 70560tatttagcag aaaacaacaa aaacaaactt cactgctaca tctccatggc agtttccctt 70620ttcactaata taaaatcact aattcatttt aacagtttca aatacttgat ttccagttaa 70680tatcaaactt ctagagatta aaatttttta ctcagtaagt aatcatgaga tatttaaaag 70740tgctttataa ataaactatc taatttcata ttataccgag agagaagcac caaggcatac 70800aaccttgagc ccatacttgt cactatcttc attaaatcct tgtcatagga aagataaagg 70860tttaaacttc catcagggga aactcatatt catcttactt catcaacttt aaacattaaa 70920attaacattt gattaacata tttttcatgc aaagtaacca atacaattaa aggaatgaat 70980tattttacca aacaagaagt tgttctacat aacctaaatt ctataatttg actctaaaat 71040tctattcttg gagattacca caaaatttaa gttgaaatgt atatctggcc cataataatg 71100tcattctaag gaatccgttt ggttctatac aagcatggga actgcaagta gaaagaatct 71160atctaatttc attctaaaac ttcatgtcat tattttagat agaactaagt gactaatggc 71220actagagata aacaaatatg gcactgataa gtgactttag gactatgtca ttctttgtat 71280gacaaagaat ggtatgtcat acgatcctaa ctcattcaga tacttatatt tagaaatatt 71340ccctgtatat ttaccatgca tagtgtacaa aggccaagcc tgatgactca agtttgatcc 71400ctgtatccaa cttggtgaaa ggagagaact aattaccaca agttgtcctc tgaactctaa 71460acacaccaaa acatatgccc agtcacatgt atctacagaa aataaataaa tgtgatgaag 71520tttaaaatgt atcacgaagt tagagatacc acttatgaga aaaattcatc aaaaggaaga 71580cttatttccc tattatttat ggaaactcag catgcttaac aacagcaaac cgcttcaata 71640tactgacagc atgcaccaaa taaaataatt accaactact ttaattttgt tacttcctta 71700acaaaagcta gtgatcaagt tataaatgta ggatgaatgt ttcgaaatat taactatttt 71760tctaaataat tgactgagtt aattcttcct agctacgttt tttctgtaag tatgtaccta 71820aacactaaaa atttctaagt atcagtacgg aagttttaaa acttcatcta cgtgatttaa 71880atagagccaa tgtttatgtt tatatccagg atttttaagc gttaatttca aagttcaaaa 71940cactagtttt ctaagagtac taggccgccc ccccaaccca tacacacaca cacacacata 72000cacacactac ttctttatgt gataaagaac tctcaatact gaagggagaa atgagaccat 72060ccaaaagtca gctcaatgta attagttgaa tgaactaatt ttctaggcct cattttacca 72120ccttatgtcc ttataatttg tctagctagc actctctaca tgccaaataa taaactcagt 72180acattgtcag gtactctgat tatcatatga aacttgaact tgaagattat cttctgtcta 72240cagctagtaa aagagaaaga cctgaaaaga tcaaatctta taattgatct tatgctatgt 72300ccattaaaga agtcaggaca attttcagct tcagaattca atttatttcc aatcactgtt 72360ttacagatta taatttcaga agaattaatt cttaatacca gcagtcctaa aaagttttaa 72420gggggaaaaa aagacctaag taatatataa ttagaaaaca aaatcttcca aaaaattcca 72480atctaaactt aacaaccatg tgtactatct gggtacagta tagaaactcc tgaataaatt 72540atatcactat acttaagata tcggtaattt attgaaaatg gatatgctaa gcactttata 72600agaagactca gtctgtaaca ggtagaaatt cacaattttg aggccagatt gtttgggttc 72660atgaacagtt tctgtatctg agttaactac ttatttagtc tctttctgtg tctcagcttc 72720ctcatcctta ggatgccagt atatttcaga attcaaatga tttaagaatt acaagtgttt 72780agactatttc agtaaatttt ggaagaggta ggaaaaaaat aggaggtgaa gatgagcaaa 72840atgcattgta tcatatatga aatcctcaat taatggaata gtatgtatta cctgctacat 72900gctaggccct aatggtaagt atcagatgct attgcaattg ctataatttc tatcatttgc 72960atgagtatat gacttggtca agagaactcc attgcactaa atctgaaaaa ttaaaatcta 73020ggcttaattc caaaatttga aattttagca ttactaaagg ggagacagca gagaaaatgt 73080ttgaaactga ccagcttttt tgcaccaagt tacaagtagt agcccactaa tactaataat 73140tatattaaat taaataaaca gaggtcataa tcaaccatat taagttgact aatttgatta 73200atgtgcgttt attaatatta agttaattaa attggttaat atgattgaat atgattaatt 73260aacaataata agctattctt ttctcattat aaatgatacc attgaatgcc aaatgcaggg 73320catataaatt cattaaggac atgtaaaagg cctctaaagt tggaaacata catactaata 73380atagcttttg ataaccttcc aatgctaatc catacatagg actggtcact aaatcatacc 73440tcatgtgtca aatctacttt ttttacagtt gaagtttaga atattttata gtcttaaatc 73500attaaaaaat tatattttgt ttcatgtgtg aaaattgcat aaaacttaaa tgtctatgtc 73560cataatttct aatgtaacat ggtaaatttc aagtacttgt atatttcctg tatgttctct 73620atcataatgt atctagccaa aagtaaaatg ctttgtcacc ttaaaatgaa ttccctgtgc 73680atgccagtga aactttcaac caatcatctg gcctccttac tttctctctt tgtcctcaga 73740agatgaatag agtaaggttc tttcttccta cttagtactt taacaaaaag ggagtgggga 73800agtaggaagg aacataaaag aaaaaccaaa gtaaagttca ggaaatgaag tgaatccgtt 73860tcctgatatt cataaccaaa actctacaaa aaggaggtag cagttacttt tgcctattca 73920tattccactg acttcgtgga aatcaaagta caatatagtg agtccttaaa taaaaatatg 73980ccttttttat ttagtttata agaatcaagt gaatcctacc agtaaaagtg ttcctcttta 74040aataatagtt tccacttctt tattactttc taagaagcca ttaaaacgga ttctacggaa 74100actgatagaa gcagatgcag ggacccacag ccaaaccctt gaagagtcct gctgaagagg 74160ggaagggagc caaaggacac cacaaaaaca tagcccacag aatcaactca gcagggctca 74220taaggggtta acagagactg aagcaacaat tggggagtct gacctgggtc ttctgcatat 74280gttatagttt tgtatagctt gatgtccttg tgtgacaatt agcagtgaaa gtaagggatg 74340tctccgactc tcctgcctgc ttttgagaac tttatcctcc caatggcttg ccttgtccac 74400cctcaatggg aaggttatgt gtgtgcctaa tcttattgaa acttgttgtt atgccaggtt 74460tggttgatat tcatgggaag cctgctcttt aatgacggga aagaagaggg tagtggatct 74520gaagcatgag ggaagggagg aggaaagaac tgagaggagt gcagggagaa gaaaatgcag 74580acaggataaa ctatatgaaa gaattaatta ataactaatt gatgttttcc acaaatgaca 74640ataaagagaa ataattttca gtgttctcag tagcactaat aacattattt gctgggtcct 74700aagttaattt cgggatagtt gaatacttag gaatagtaag taaatcataa taaaaatgat 74760aaagttataa gatattcata actatatata gatagcatca aggagattat acactagaag 74820aataaagaat tcccacatag ttcagcatga gaatcaaaaa cagtgaaata gctgccatga 74880aaacaagaaa cagaaacatg aaagagataa ctaacaatat gacttattca aagtatcttt 74940tttaaaaaaa taagcagaat aaaaacaaag attggacaaa agaggtaaga aacatataca 75000gcccaagact caattaaccc atgcataatc tagtaagcac tgcttagtgt ctttcatagg 75060taaatgtctc attattttgt aaggggtttt ataaaggatg tacctttata aaaatcacta 75120ctcctccaag aaatggtggc ttatgcttat aatagtagca tatactagac taaggcagga 75180agatcttgag tttattatga actttatgtg gaggaatcag taaaccaaat ttcacaactt 75240gccccaagtt atttttaatg tagaaacttg tcaagagtaa aaaatgtgat ctcacaaaca 75300ccagagatat tcataataca gttaaaacca gtataaggta gggtctattg aagtgtcatc 75360aaatcatgta ttacaagact gaaagtacaa atatgaccca attagtatca ggaaccattt 75420gaaagtcaat tatatataaa ataaggaaaa tttcttaaag acagatagat gggtggatgg 75480atggatacta aaaatgcttt tattcatctt tacaacaaag tactagttgt aaaaatagaa 75540ctgtggaatt aaattttcac atagaattat ttatctttta aaatgaataa accactgttt 75600ttggggtttt ttttttgttt gtttgtttgt ttttcacctg cccagtttca tggttagaag 75660aaagtaagta aacatttaca aatttagaaa ttgactagtg gagctggaaa catagtgtag 75720tagaggcaca ccaccaatat atattgtata aatatataaa actttcaaag aatattattt 75780tttaaacaaa gatcttttcc taaaaccata ggttgactct tctttctgta aacagaaggt 75840gaatattggc tgattgtagt gaatgctagt tttagaactt gtatatttcc taagtaagaa 75900ttttgaaaat ggaaatctgt cctgtattag tttggaaaga gttagtacca ctgccataca 75960aacagcagat acctgatagg agactagagt gatggctcac tagtcaaaag cactagctgc 76020tttttcagag gaacagagtt cagttcctag catgatgtca ggtggctcac aaaactgcct 76080gttcctccag cttaagagca ttaaatgcca caggcaccca cacacatata gcatgcatat 76140gcatatatgc atatatatat atatataaat atatatatat atatatatat atacacacac 76200atatacatat acacatatta aatctaaata cagtcaaaac caatgcacaa aacacatttg 76260aaaaagaaca catgtccttg ctaaaatccc attacttaaa agaaatcttg tgaagccaaa 76320aggctttcag acagactcat ttgagaacat gaataaccac tcccagtgat agagatatct 76380cagtagttaa gagcactgat tcctcctctt ccaaggaatc cagatttgat tctccacacc 76440aacaaactgg tgcagtcagc tgtgacacct ggaggagtta cacactcatc tggtctccat 76500gggcaccgac catgctacat gtggtgcaca gagatatatc ccagcaaacc acagataaac 76560ataaaaagga ggaggaagag gaagaagaaa tggaggagga gaaatattta ctcttaccac 76620ctctaatgga tgaagtacaa tatctaggca tagctatcag ataagaaaaa gataaaagat 76680acaggaacat ttggaataag atgcaagact acaaggtgct aatgtgataa agtgaatgaa 76740aacttccaga gtatcaaagg ataaattcaa attctaagag agatgaagaa tattggaaat 76800cacaaaagag atactacaac tgagaaacag agaaaaatac tatgtagcaa aaaggaaaag 76860cagcaagtgg ctccagccaa gtggtggtga taagggatat ctcatccata aggtaaaaca 76920gatgggactg actaaaacaa tgcaatagtc ttgatcacag aacaagcaag agtactcaca 76980aagaattaat tggaggtttc gtcatgacac tgacttattc taagtcagca ctggaaaggg 77040aaaaatccag tttataactg tattctggag aactagtcag gtgtcatgaa aagggatcta 77100catttgtttg aaaacaaaca atcttggggg ttgaaaaaca tgaaacaatt acaaataagg 77160aaaaggggtg aagtcttgcc atagtctatg ggtgggaaga aagctgagtc aggtagatat 77220ggagtagaga cttgattgac tgagaaactc aagcaaagta gacaaggctt gaagggcact 77280gtgtcaatta accagccctt ctgtggcaag gatcaaaccc tacaatgtgg aggataaagt 77340gtcaagccta caaaattggc tctgtctgtc atgagggttg ctgcctacag tacttgacaa 77400ctctgaaggt aaggtccagt ggagaaattg attccagctg tctgataccc cttgcaatgt 77460ggtgacgaca ggcaggatct aaaagtatag agaccagttc tctagaacaa acagtgcttt 77520tttgacttcc ctcatttaaa gcatacttgg gagacaccct gtggcttgaa agtagccaac 77580taagacagaa aagctggaaa gtccctgaag gaaataatga atgagtgaat gaatgaatac 77640ataaataaat aggtgaaaat ttaaaaaata aatgtaaaaa tccctaggtc ctgtacagca 77700aagttcagct agcaaagaca ctggtattca aaaacactga caatctcaaa ccgaagatag 77760ttttctatga catctgagct ttgactggtt ccaaatcagc accagcctca ataacaggac 77820tggaatatta ggggatgcag aataaattac tccaatacac ttccttttac ttgttattgt 77880tttagacttt attttttgta agactattat ttacttacta ttctatttat ttgtttgctt 77940tctattttgt ttgctttctt tacttattga ccatttgttc cattcccatt attcttagta 78000ttctacttct tttcatttaa acttgcttcc cttttctttt cctgcccctt tccccttttt 78060tttttcctta agctgctatt tctaatttga ccctaaacaa gttttgacta cttacattat 78120tctaaacata tttttaatta tcaatggtgc tataaatgct ggcatcaatc aattcaaagt 78180ctatatctga aaattgtttg gttttattgt atgtgttgga ggtttctttc ctcctttgag 78240tagtgttaag gattgaaaac tcaacagaag gctaattaca aataaaactg gaaaacatat 78300ccatactgac aagtgtacaa attagaacac aaaaacaaac aaaacattat aaatggaaca 78360aagttatttc agaagatcag catttaataa ccaataaaaa gatggccaac tgtttgaaat 78420gctgttcaaa gaattctgaa gtacatgtca atgagaagtt tagcaaagaa attgagactg 78480gtgctgggag ggaggatgat ggaaataaat aaaataatta aaaatctcag tggaaaccat 78540aaataataga ccaaatcaag tacaagacag aacagcagga attgaagaaa aatctgtatt 78600cagacacaca acaacacaat aagtaaacac aaccaaacct tttagaacat gatcaaaatg 78660aaactaaaga atacatggag cagaaggaac ttaaaagcac tgagactaag tcaatgaaat 78720aacagcagaa attttctaaa atatcgagaa agaaatgaac atctatacag aaagatgtgt 78780aaaacccaaa taaatcatgg acagaaagaa ttgctcctga gcacagtgta gctcaaatcc 78840taacactaca aagaaaataa aagtatcaac atactaacaa aggtaaattc actgtaacat 78900ccaacctctc actagaaatt tttaaagaca aaaatctttt caatgattac tatatccaaa 78960gttatctttt aaatgtaata taaaaacttt ccaaggtagg cataacataa ggccacttgt 79020gaccactaaa ctacagtata cacttaaagg aaacttacac atagcagaat gatacaaaca 79080gaggagctca aggaaaatga attttgtaat tctatatatg aaaaattaag aaagaagcaa 79140ctgtgtctac ataagttaat gaaagaaaaa cagaacaatg aaagaaacaa caaaatataa 79200atgtttttag cgttccattt atgagacaga ttagccaaat gaattaaaag caggatcaaa 79260ttatttgttg tctataagaa atgtatttca cttgaacaga agctaaagga aaaaaaaaag 79320gagactgaaa agtgagtgga aatcaaaaaa gaactggact actgacataa gtatatgact 79380gtatactata aaagaaaaca actgggctgg agagatagct cagcggttaa aagcacctac 79440tgctcttcca aaggtcccga gttcaaattc cagcaaccac agggtggctc acaaccatct 79500gtaatgagat ctgacactct cttctggtgt gtctgatgac agctacagtg tacttacata 79560taataataaa taaatctctt taaaaatata ttaaaaatgt aattgaggaa aatacctaaa 79620aaaatatttt aaaaaatatt taaaaaaaga aaacaaccaa tgagggatgt acaattaatt 79680ataagtatat atgcattaaa tgatggtgta cccaagttca taaaaacaag cactaccaga 79740ctactgtaaa atccaacctc tcgggctggt gagatggctc agtgggtaag agcacccgac 79800tgctcttccg aaggtcccga gttcaaatcc cagcaaccac atggtggctc acaaccatct 79860gtaacaagat ctgacgccct cttctggtct gtctgaagac agctgcagtg tacttacata 79920taataaataa ataaatctta aaaaaaaatc caacctctca ccagaaagta caatatccat 79980atagaaagta acaagtcaaa atacagtaaa atacagtaat aatcttaact ttccaaatta 80040ataacttatc tgatggaaag aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaaga 80100ttgattttat ctaaactgta ccaaacatca aatggactta tttcaagaca tctaaaaaac 80160atctcacctt ctgcagaata aaccttggca gcccatggaa ctctctaaga gataaaccac 80220atttttgaac attaggcaag tcaacaatag taaaaattac tttcttgtac ttaacaaata 80280ataagagaat aaaattaaaa tgcaaaaaaa aaactaggta tgctacaaat acctgaagac 80340aatataatac aatcttaagt atcataagag taactgaagg aatcagaaca gaagtgtttt 80400aaaggaataa gtcaaatgaa aagaaaacac actaggaaac agcaaaatca tcctcagtga 80460agtaactcag actcagacag ataaatatgg tatgtattca cttataaaaa aaaaggatat 80520tagcagttaa gtaaataata accaaggtac aatctataga gccagagagg ttaggtaaag 80580aggggggatc cagtagggat acaaggatct ccctagaaga gtgaaataga ttatacagcc 80640aactgggagt agatagggag agtaagcagg tgtggggagg tgagcaggtg gagggagaga 80700tggggttgag ggaaagtgtg gtagaaacag ctggaattgg ggaggaaaga atttccatgt 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81600tcctgtaaaa tatgctaagg cagtaggggc aaagaactag tgggagtagc caaccaatgt 81660ctgatttgac ttaaggccca ctccatgaga agaagcctat atccagactg cttggataac 81720taagaaccag aaacctaggg taaaacaaaa acactactta cgttttaaat ttttttttta 81780aatcttaaaa aattttaaag tcaataaaat gattatggta tacacattgt atcagtgcct 81840aatctagtta ctgtcaaaag aggttttctc tggcagcaga tggaaacagg tgcagagatg 81900gagagaccac agtcagacat tacatggaca gaatatttaa atgggagctc tcaatcaaat 81960actttacagc agagagcagg gaagcccttg gaagagaagg tggaaaaact ttaagagcca 82020gaggggatgg acgataccag aacaagaccc tctaaatcaa ctacgaaagt ctcataggaa 82080atgtcataaa ctgaagcagt gggtctgtac caggtatgta tgtctgtatg tatgtctgta 82140tgtatttgta agtgtctgtg tgtattatat atgtatgtat gtatgcatgt atatgtatgc 82200atatacatac atacatgtac tacatgtatt acatatatta cgtgtattac atatatatgt 82260agtatattta tactacaaat atgtatatac atataataca cacacacata ggcacacacg 82320catgtgcaca caaacacaca tgtaaaatag ctataggttt agtattgtat ggtactccct 82380ggtgtgagaa cgaattaggc ttctgactct tatgcctgct cttgtgactc ttttcctctt 82440gtttaccaag gcagactacc atgatagttt ttgcttcatc ttattgtatt ttattttgtc 82500atgttaaaaa aaaaatcaag aaaggaaaat atattatgat acagaaaggc atctctaaca 82560gtgaagtttc actccttcct ttagaaaatt cggtcagaat tcaaataaaa acttaacaat 82620gcacatcaca atctcagaaa aataagaaca acccaaacta caaactaata cctggaaaaa 82680aaaaagaata aagaccagta tagaaattaa tgtactgaac tctaaaacac agagttcttt 82740ctttgaagag aataacaatt ccttagccca aataaccaaa gaaaggcaag accaaattaa 82800taaaattcaa ggttaagatg aggacaaaat aaaatgtagt actgaaaaca catgaggtct 82860ttagtaatta atatgaaaag caacatttaa aaagaatgaa gtatctagaa gaaagttata 82920aattcctcaa caggtatgat ctataaaaat tatattaaaa ggaaattaaa aaaaactaaa 82980gatacagaaa gcaacaaaac tgaagcagta catcctacca ataaagaaaa gcaaagaata 83040gattcattgc taaaattacc agttctttat agaagaacaa acggcaaaaa acctcaaagt 83100gttctaccaa acagggaatg aaaactacta aagccattct acaaaaatat tattttgaga 83160gttccaaacc aggtaagagc acttagaaaa gaacacacct caaacatact aaaaggctat 83220aaaataacct gagactattg ttatgttaaa taaaaaaaaa tactgaagtc atttttctct 83280aatatcagac atgtgacaag ggtgttcagt ttctataata ttattcaatg tagcactctg 83340aagtcataga gaaaacaaga aaaaccagaa caaaacaaat gggaaagaaa agaacaaatt 83400atctctgtaa gtacacaaaa gcattcttta cttaaggact ctgataaaca cttgtagcaa 83460aaagaaagac ataaccaggg cttttctata taaccctaca aagatcccaa gaaacccaga 83520gagacagagt gacagagaca gacacacaca gagagagaga gagagagaga gagagagaga 83580gagagagaga gagagagaga gagagagaga aatgaatata gcaaaaactg acaaatgaga 83640ctgcaacagt taaaaggcaa atcagtagtg aagagacaac caaaataatg gaagaaaatc 83700tttaatgcag aaaactatac atctaacaga attagtatct agaatgtcta agaacaaaaa 83760ggcacgtgcc aaataaactg atattccaat cctgcactaa attagccctc ttcctcaaca 83820ttgtaaaact cttctgtgat aaaaatagaa caatccaacc caagggcata tgtactggag 83880atctgcttta gccccacatc tgaggtggga cggcatgggg tagggagaga tgccttcttc 83940tacccccctc tcactaccta cagcaggtag aactggccct ttcccttact gattgaatca 84000ctcaggagca caggtcctgc aaattgacta taacagctca gaaaagctgg ctatggtgat 84060gttgcaggta agctggatgc aaggacattg agagcacaag ggctgatcct gcttcattgg 84120gtaagttaag caggatagtg cttgagagtt gacctcggct gagaaaaata actcaactac 84180ctcccaagca cagacccagg gcctacccca acatctactc caattatgat ctgctggaac 84240ttatgaagtt gccagttctg cacatccaaa actgcaggat ctccatgaca cagggtaaca 84300acaggatatc caggagtcct ggttgaggat ccaatacggc agaacctaga ggcctcaaaa 84360tagaccaata actcattgca atgaacattt gcaagtagag atgtgtggac aaaagcctag 84420aaaaacacaa tacaaaaggc cagtaaataa attcttccca acatattata gttaaaaaat 84480aaataaataa aacacagact aaaggatact aaaggataca agaaaaaagt gacatcactc 84540ataaagcagg ctcatcaaaa ttaatatcta ttcaacaaaa gtctttaaag catgaagtgt 84600ctggatcagt gatccttaac cttcctagtg ctgtgaccct ttaatacagt tcctcatgtt 84660gtgatgactc ccaaccaaaa gattatttca ttggtacttc gtaactattg ctactataat 84720caatcataat gtaaatatct gatatgcggg atatctgata cgcaacccct gtggcagtta 84780tgactctcag gctgaaaaca gctagcctgg atcctgaaag atcacaactg gtaacacaaa 84840gcattacact tgtaaaactt tacacaaaaa ttcaaagaca aatagaaatt ttccatgata

84900aacagaaaaa acacacatct ttcaagaact ctgattgtaa atggtcttaa ttaatccctt 84960ctccaaagat acaaaataaa gaaaattaga ggaaaaatct accaggtgga tgttacaatt 85020ttagacacat aagcaacaca gaggcgcaca atttcatagg aggaatgcta ttagatctaa 85080aaccacgtgt cagtctcaac agtgactgat agtgggtgac ttcgctaccc aactctctcc 85140agcagacaga tcatctggac aaaaattaaa acagaaactc tggagttaag tcaaatgaat 85200gtaatagata tttgtacaac attccaccca tatactaata cattcttctc agtctatgga 85260attttctaca aaaatcaact acctatcaag acactgaaaa agtcataata tatgaatact 85320gtaataccat acttctttgt atagaataca atggaataaa attagatatt aacagcaaaa 85380gatattacag aaaccatggg aactaaataa cacattactg aacaggtgat ctctgtgagc 85440tcaaagcctt cctggtctac agagctagtt caaggagagt cagggctaca cagagacaat 85500tatctcctct agaaactaac acccacagtt tattcatctc tgagaaaagc atcataaatg 85560atgcacaaga aaatatcttc aaacagcaaa tccctgttaa aggaactcaa agacaatata 85620aacaaatggc tgaatgagaa ctgcagaaac atgtaggtga atcaagtaac acaatcctga 85680gaaccttagg acacagagga tatctcaaca taataaagga aaaacagaag aaattcctgg 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86580agagcatgga gacattatgc tggtactcac tggaaaactt tatcacaact ggctataatc 86640cactgtgctg gcaagtgcta tataaactct cttgagaata aaggcctcaa tctcatccag 86700ttatgaaccc tgaaggctaa aagtggcctg acaagatagg attactaata attgcatagg 86760tgttttagga gtaactagcc actatatgat tgcattaaag gtctactcta taaagtggag 86820gccatactat taaaggagcc aagaacttgt gaatagataa ggtaataggc cctagggtag 86880aacccatacc attactctgc taaatgcaca taatattaaa atgactccta atgattattg 86940ctatatcccg agaaaagccc attattttag ctttcactag agaagctttg ctccgtaaca 87000tatggcaagt aacagagatg atcctattcc aaaactggta agcatggcaa gaaaaagaaa 87060gtgttaagtg ctcaatccca aatcagacat ttatctcacc ccaaagagct ggaaagatgg 87120tcactcacag gtggtaaata acttcaagga aacagtcttt tccaagagca agagactaga 87180tgcatatatg aacttatgac aagtcattac gataatacac ccaagctaca gccagccaaa 87240accccagcat gggggggtgg gggtagagat gtcatcctac cacaagtgtc ttatgaaccc 87300ctggtaatct gactacacac tacagcaggt cccaattcca agactagcct gacaatgcaa 87360aactagactc aatggagaat aaaggaaaaa aagtttgaaa aagtataatc ttaaaataat 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88260ctctcataaa aaattctgaa aaaacattca aggatcaaca aggcaaagtg atttgtcaaa 88320tggcatattc ttaagatgtt atgtattatc cctcataatc acaggctgag aatatgtaat 88380agttatttag aaataaactg ctaccacaat atatttctga gacactaaat atttttcata 88440catttaatga tttttcacta acacttagta agcaagcatt aactcattaa ctaactattc 88500cttatatatt ttgctacgta ttcaaggtag ctttagttgt gaatttgata gtctaatgtg 88560tagtgtgacc taagggactc tcaattgagg gactacctag attaaactgg cccatgggtg 88620tgactggggg aaaggggaag gtcttgattg atgtaagagg gaccagtgta ctgtggataa 88680tagcattccc taggcaggtg gttttggact gtaagtaagt ggtgttagca atgcttaggt 88740ctgaaaattg agtcagcaac agtataaagt tcctgccctg actactttga acgatggcct 88800ataacatgta agctgaaata aaccctttac tcccctaagt ttctttttgt tagactgtta 88860aatataaaca gaacattagg ttcccattct actgagacct taaaagaaat gaagtgatac 88920taaatctcag aaaggaaaat agtaagaatt gagaagatgg gacacaatca tattttgata 88980gagatactca gaaaagatac ctatgacagg cattatctaa gaattctgaa acaagtaaaa 89040aagaaaaaca cgtagattaa ctcaagaggg aacagtcagt aagttccaga actaaaacct 89100caaaagttgc aggttaagaa cataatgcta gtacaggatg tcacataaat gaaagagcca 89160gttcagggct gtatcaattt ctaaccatag tttaagaaat aggaaacctt tgaaaagttg 89220agtatagaaa agatggctta actttttttt aatcagaaaa gcccattgaa ttgttggtag 89280taaatagagg taggaacaaa agtactgaga aacaatgatg tcccttatca aaagatggca 89340atggtactac aaatgctttt taaagaagct gaaatgtatt ctgaagataa atgcaacata 89400acttgcaaac agttccaata tgtgacacaa agagaaacta aggatgcttt ctagaatttg 89460gtttaaaata aagtcctact aaataagatg caaatgtcta tcagatttca agggattgat 89520agcatacaaa taacagttct attttggatg aattgatttc tggctttttt ggggggtggt 89580gtatacatgt gttgtgcaca caaacatgga ggcatgcatg cccatgcatg tgcatttgga 89640gaccagaaag caaaggcggt cataattttc taatgctcct cacacaattt tttaagacgt 89700ggtctctccc taaacctgac acttgctgaa ttatctagac tgaatgaaca catttcaacc 89760accattccat ttctgcccct ccaccaaggc tgggatgaaa ggccttgcct cactttttac 89820ataggttctg aaattctagt ccacatagtt ggaaagcagg acactgagcc aactctttag 89880gccctgcttt tagctgcttt ttgtgtttgt ttgtttgttt gtttgtttgt ttaaagccat 89940gatctcatgt aactttgact tgccttactg tataggcgaa gctgtccctg aataagctcc 90000caagtaccgg gagtacagaa gtggagcaag caaacatacc cagccaaata tcaaaattct 90060taatacttct atatgcacaa attcataata tgaaattttc atataatcca cctagatatc 90120ttctggtttt ctttcttctt ctattaaact catgggctaa aggacatcaa tgtcaatgtg 90180catgttatat cccagtatta agagtctcca aaattatttg tatttcttaa taagagaact 90240ttacaatctc tttatttcca accataactc ctttatctta agcataataa acaccatatg 90300taggattatt aaaatgttat tcgtctgtta aaaacactca ctcatgcaat aataatttta 90360atatatgaag acacggctgt ttttccaaag tttttatttt cattgacaaa taagaactac 90420atatattatc atatgcaaaa gtgatacatc aaatgagttt ataaattcta acatagcact 90480tttgcacata tacttaatag ttgactttta gtgatattca agaaaatata cagaaagaag 90540tcaccatgct ctacaatttt ggtcctgaac tacattctat tcattttgca ttttttatgt 90600ctatcattta ttctactcta agtaacaaac aatttttaga atgtttttac agtctatgta 90660caggaagatg atgcactacc tgtttttgtg ctgatttatt tcattgaaaa tataaaggat 90720catttccatc catgttgtca ccgacataag atttcagaaa aaaattttaa tggcgaaaaa 90780ttacatcatc atatatgtca atttttattt gtttagccac tgacaaacac tggtttattt 90840ctgcattttg tttaattaat gctgcaatca aaattaaact gcaggtatct cttataacac 90900actatatatt tctataatac aattgctagg tcattgcaat ttttaggaac ctgttatttt 90960ccataatcga tctaatttac attccaaaca actgtgtaca agatttctgt ttgcttacaa 91020cctcaccaac atgaacattc atatattcta gcaggtagga agtagtatct cactgttgtt 91080tcatttctta gatgattaga aaggtaaaga attttttttc tcatgaattt gatgctggtc 91140atggtggtac aacaccttta atctcacact cagaaagcag aaacaggaga atatctgagt 91200ttgaagtcag ccttgtctac agagcaagtt ccaggacact caggctacac agagaaaaac 91260ttgtctcaaa aaacaaaaat aaataaatag gtttttcctc aacacagaaa aagagcaaga 91320attgaaatat ttgagattct gcaaagaata gaggaaaaga aaaaatatta taatatcaaa 91380ataattttta aaaactcatt taaccacttt gccatttgta ctcctttttt cccctgtctg 91440gtctatacca ccttacttgt catcctctgg actgtagtcc agttccacat tctttcacca 91500gcatagagct taccaatcag attagcccca tggatcctcc tggatcttcc tccccagcac 91560cggaatttta tgtgtacact accacacact gcatttttat gttgttatga aagttatcaa 91620atcaggtcct tatgcttctt aggcaagcat tttacctact gagctatctt cctaccggaa 91680ctacttgttc agaagcacct atttaggtcc cctatccact tcttccatga gctgtctgaa 91740ttacacattt cataaagtaa ctattcagca tgtgaatgct ttcattcatt ccataggggg 91800gttatctgtt aactatatct tttcttgtgt agaaagctat tgttttgtat aaccccatta 91860gtctattttt tgtagttatg tttttaaaac aaaatttaca aacactaatt aaaaagcttt 91920tcctgtttta atatattagg tgatacttaa aagaggatat taactaagga cacaactttg 91980tcctatttaa catactggtt tttttattct gcaagagtgg tcagtgctcc taatagctga 92040gccaccttgc agcctcaaga atgcttgagt cagagagtaa ggcaatatgg cccctgtagt 92100taggcagact tgggttttga ttttagctga tgttgtttct tttggggatt tcaagacagt 92160gtttcttgat gtagctttgg gtgtcctaga acttcctcct gactgtctct gcctcccaag 92220ggattaaaga ttaaaggcat gcaacaccac catactgggc taaaataaat tccacttaat 92280tataatgaat aataattatg tattgtttaa ttcagttggt tattcctttc tgaagaactt 92340ttgttactag cctatagtac tagacttttg ttttcatatg agagtaatga cttaacttat 92400agtattagtt cttcaaggtt tagtgaattt catcagtgat atcattaggt tctgggcttt 92460atgtgacaaa agacttaatt cctgattttt ccatcttctg atgacacaac attctcaact 92520ctggccatca ataatagcat gcaaacttaa aatgctctaa tgctaaaagt gttatacatg 92580aaacactact aatggtttgt cctatctatt ctgctaatat tcaatctaaa atattatcta 92640ttagagacta ctggtacagt ctaactgcaa gcactatgag gtttctaaac taagcaggcc 92700attcagtgca tgaaactttc aacatttggt ctgttttttt aaaacccttc atatcttggt 92760aagttttcag tacactcaat ctcaactcaa ttataactat agtgttccac aaaacatcat 92820taaacctttt tcaaaagaaa aagaaaaaga aaaagaaaaa ggaaaagaaa aagaaaaaaa 92880aagtacatgg ggagcaaaga tgtggtggca cacactttaa tctcagcgct taggaggcag 92940gggcaggcca gtctctgagt ttgaggccaa attggtctac agagccagtt tcaggacagc 93000cagggttaaa cagagaaacc ctatctcgat ggggaggggg aaaaaaggta catggggaag 93060gcagttcttt agtttctact atacctgcag ctatctaggt atacaaaata aacgagtttt 93120cttttaaaca acaacaacaa caacaaaagt tatttggcta ctaaactaca ataaaatata 93180acctcacatc cattaggatg actattgttc cccaaagaaa ttttaatata cgggtcaagt 93240tgtaaaaaaa aaatgtataa aacttttatt ctaagaaatg taagtaaaat ggtatagcta 93300ttgtttaaaa cagcatgata tattctcaaa atattaaaaa tacaattacc atgatattct 93360ataggaccaa aagaatttga caaaagatta gacccatgct aatcacaaca ttattcacaa 93420ttgctaaagc acagaattaa cccattaaca aatgagcaga atatagcata tacaagcaag 93480caagtgttat tcagccaaaa aaaaaaaaaa aaaaggagga agttctagta tatgctacac 93540ataaacttca agaacactgt gttatataaa ataagccagt catcaaaaca aaacaaaaac 93600tgtgtgacta cacttacttg agatagttag aagcaccaaa cccacagaca aaaagtagaa 93660tttaattgcc agccacagtg gaagaaacag gtcactattt aatgcataca aagtttcagt 93720agggcaaaat gcaaacagtt ctgatgacaa atgctcaaac atattaatat ttacaatacc 93780actggctcat atactttaga acaataaaga gtaaatttta tacattttat aagtgtttta 93840aagttatcag agaagttaca tggtctcaaa aatttaaagg ataattcata gttgtttctt 93900aagacttcac aatgtactta agcatttaaa gggatgtaat ctgcttctag taaggaaaac 93960ctggactttt tcaaccacac cttaaacata gctatacaca tctatcttta cttcctcctc 94020atttcttaag acaggaatgc atatgtgtgt gcattaacat agatggtata ggtagattta 94080tacatgtaga gaggtgtgtt tagaagtcag aggcttgttt tcactatact caaagcttgt 94140caattatgct aacctagcag ccagcttgcc tcttactcca gtcttcttaa tacttacata 94200gcaagtgcta tattcaccaa gtctttgcca cagtcccctg cttaattcct tctagttatt 94260cctattttca tttttcattc ctcattgtca aattttgaaa aggttctcaa tttcctactt 94320ttccctacac tcaagtgtct tgcttcagaa ccttggcaat gacttggcct agaacactgt 94380tcaaatatcc aactgcttaa tccccagtaa catttcagga ttcaacctag ttttactcag 94440ctataccaat gttcctacta tgcactagat ttcttttgca taatttggta ctcctctgta 94500ttcccactgt ataccaagta taattctatc tcaataatta aaaaatctga ttctgctctt 94560tctgaagcat tgagatataa gtctcaagca tctttatacc caaaacatag cacctgatga 94620ttaatattta gaacaggggt catatttaaa caatcttgct ttcaggaatt ataaattaat 94680agcaggcaca tagatgtttg aatcttcctc taattcccct atttatcaac aactgccaag 94740ggcacaaaaa acattgaaaa ttcagaatca tgtatgtaat tatctactta ccattagaca 94800tctaattttt tgcagaggtt tggaagtatt tggttaggag atataagtag acatgttcca 94860atctctagat acaatttaaa gttgttcctt ctttttcttt ttttaattca attttacctt 94920tttttatttt aagcctgaaa ggtattttca aataaaatag tgtagggcaa cttgagacac 94980aaaataacct gaacgaagtt atacttacat tcggctagct tcaatgtcct cagactggga 95040ttctcctggt gatctgtaaa atgtaattaa agacaaatat tagtgatttc caaaaaaaag 95100gtatagaaat ataagtaagg aagataaact ggaaaacaag acttaaattc caatgaaagg 95160gtgctttaaa agatttaaaa ctaagaaaaa aaaagtagaa agatcagatt atcacaacac 95220atttaagaac aaattttaga aacctcaatg tttaaaaaga aaattaaaat ctttatattt 95280agatatcaaa ttagtataag ccataattat ttacaatgtc aaacacatat taaaaaacaa 95340gactgttact ttctattaag ttgggttgtg gtattcatag tcttgaaatt aaatgaagtg 95400tttgcaaact gtaacctgag caaacactaa ggtgtaggat aatcaccttt attttgaagt 95460gatattatac tgagagattt gtattcattt atacactgtc tatgtattca gaaaaagcaa 95520ggataagtac ttgcagaaaa aactatttcc ttcaaaacct aaaacattat aaaaaaaaaa 95580aatcccttga aaaatgtctg ccaatttctg cagtagacaa tttaaactca aatataacag 95640tgttcataga tctttaagat atttaaatgc tatgcaccat tctaatcata attcctgtgg 95700ttctataagg aaattagatt tccctaaaaa gcactatctg ccgtcaccgt gtatatggag 95760cattatgttt gctagataat tgtaggctag ttttagtttt aatatctgct ggttattttc 95820tttatttttt aatatattag ctaatataga aatacatata aagcaccaaa gagtatccca 95880tttttattgg gtggaacata agccaatttc cctattctga acatctggtt aaagaacaaa 95940ttccagtgat gcacacagca atactttgct atacttcctg tcatcagaat gctaaaccag 96000gatttagcat gaggacaggc tgttctacat agtgagacac tgcctcccaa aaagaaaaaa 96060atctgttacc ctacatatct ttactgtgag acaatcaatg ttctaaggta aatttatttt 96120aatacctatt tctaatgctt taaattccca aaataggtta agaaagaaaa gaataattta 96180aattttcaaa tttgtataca aagacaatct gtgaatttac tactgacttt agtctttcaa 96240gttaaagact tgatttgtaa atttatcact gacttcagat attcaactta aacaaaaagt 96300ttataaaagg ctggttgtgg cttatggtta tggaagattt tttaaagcaa tgattcttaa 96360gttaggggat aaagaagagg ctcactagtt aagagcactt actgctccca gagaaatcaa 96420ggtttgcctc ttagcaccca cacagctgct cacaaccatc acaaactcca gttccagcac 96480tgatgccctc ttctggccta tgtgagtatc aggtgcacat gtgcatctgg tacacacata 96540catacctgca agtaaaactc acacacagaa aatttaaaaa tcttagccag gcaatggtgg 96600cgcacgcctt taatcctaga agttggaggg cagaggcatt ggatttctga gtttgaagcc 96660agtctggtct acagagtgag ttccaggact acacagagag aaatccctgt cttaaaaaaa 96720caaaaaacaa caacaaaaaa tcttaaaaat taatgtattc acttctttag tgtcaaagaa 96780atttcacttc tttgcttgac tttcaaaata atactcattg taattcattt agattccaag 96840tcagtaacac acacacaaag aaaaaggaaa aaagatacga ttaaaaacag accccaaaag 96900ctcagacagt ttagattttc ttagtagttt atttatattt ttctgtttat cacaaaatta 96960cataagaatt gaaaaatgta gattaggagt gatatcaaat aaaaaattct ccaccctctt 97020cccccagaaa acaagcaaat atctcagaaa taatgatctc tacagcctta agaaactacc 97080aagattgttt ccaaacttat atctcatata aaatggctag aagagttaaa acatgatggt 97140aacccatgca ctagctaact cattttggaa tgtgtctttc caacatctca tatattatta 97200agactgtgag ataaagctga cttgcaaaac aacaacaaaa acctgttagc aattaggaag 97260ccaaattaga agtctctaaa caggtaggaa gcaaagtgtt ctaaatgtac aggaaaagaa 97320aaatttagag aaggttaaga atgatgagag aaaaaatatt atataagaaa atataagata 97380agagaaaaaa agaactatat ctcaagagaa tattatagaa ctgctgcagt ttcaagaccc 97440taaaatgaac tatagaacaa acatattgtc aaatggccaa ctacagctat tttcgcaatt 97500tctgattgct acagagtatg tgaatgccag taacataatg ccaaccaagt attgtaaata 97560tactagactt ctatttaata ttcacaaata ttagagtaag ttattaagca ccatttagcc 97620aaactaaaat ttaagaaacc ccagaaagat cagagtaaaa ttaatccaaa caaattattc 97680ttcttaaatt atactcttct tgatctctta gacatagtat agcagaggct gctcccattc 97740aaacagtttt actttagtca agagaaaaat atataaatac aacaaaagca tgtttctgat 97800cattgtgtac atctgaatgt ctatgcagac aattttaata atattctaat taagttgcca 97860aaatatatcc taaaactaga gttaactttc aaagtaactc tatttttata actattagta 97920ggtgttacaa accacaaagt atatgtaaca cctactaata gttataaaac tgttctctgc 97980tcttaaaatg cttgctgctt tttactgaca atacatttga aataagaatt caggacatgc 98040tcctaatttt accaaattca aaatatagaa ccaactaaaa gttaataaaa aatccactac 98100caacccaaga ctgacccaag ttatcctaaa gtttaccaga atattaataa aggaatgaat 98160gaaatttgaa acctgaaaaa tacctagtaa caatggtgag gaaaaagtct tttaaaagta 98220tcaattttag gtattaactt taaagacaga aaatgtaatc acaaaacaaa taatctgaac 98280actggaacat gacctagtaa atccatcctt ttaaggaggt aaaggtgcac ttttaactcc 98340actgtaactc tctaggagaa aaagtacact cattttacta aaggtgcaac atttctggag 98400aagcactgaa actcctgcca cataaaatat taaaaataat acttaagata ttttccacat 98460atctcttaaa atgtccacac tgtaatcttc aaaatactta agacatattt tcttattaat 98520ctatgcagaa aaacaggtct ttgatgatta cttatcttca tgaactgaag ggtaagtaag 98580aaatgcacta taataaatat ctgagaaagg aggttaaatg aagaaatggc tccgttctta 98640ggtcttttat tcttagcaat attcctgcta cctatgcatt tttgtcattt ttatagcaca 98700taattcatct tacaatgaca gcctttctaa gactgtaaaa taacattcaa aattaccttt 98760tacaagctgt ggccattcgg tgacatcagg gtgataacag ctttgagtca ctgattaaaa 98820acaggttgtc acaccagtct agctgctaac ttgatctgaa cgtaggctta ataattctaa 98880taaattaaac aaacctttag ttagaacact agcataaacc caattcattg ttcaagtcat 98940tatcttcaaa gctttaaatc agtagtcaat ataaaattag aagaataatt atcacaggaa 99000aacctttatg aactattctt cagtatattg ttttcttaaa tggtctcttc atttgagaaa 99060atgaggtata ttctcaatcc aaaactactc caaaatccag aatgttttaa atcatcatgg 99120cagcaaataa aaaagggctg gatttaggaa tatttccgat tttgaatttt cagactaggt 99180atgttcaaac agtaaagtct ataggaacat gccaaaatct gaaaaattct taaatttgaa 99240acatagtctg gtcccaggca tttctgataa agataataat ccctactttt caatgtagta 99300ctatatggct cacaaaatac aacaatcaac tgtaccctta cattccactg actttagctc 99360tacaagatca aaatagaaga ataacaataa gcagcctcag agtgattaac tgacttgccg 99420aagattttgt aaataatgtt gaaattaaga ccaaaaaacc aatatcagct aaatttccac 99480taagtattag aattttgcta tggtttagtt ttgctttaca gtgttttgta atttaaatac 99540atacaaaaag caaaaattta gactatgtat tgtggcagta attagaatga aactgattta 99600gtatcaagag tctttaactg atgggagaaa tcagctacca aacaggcaaa atcttgatta 99660aagttttaca cacacacaca cacacacaca cacacacaca cacacgcacc cacacatgaa 99720agagagagag agagagagag acagagacag agagacagaa attatttaag tagtataatc 99780tatcattaac ttttagacct ttggatctat aagaaagcat tcgttttcaa tcccactaag 99840actcaattct aggaagtaat ataaagcaaa atatgaatga atatcctatt attcacatat 99900ctgaactatc atatgtgatt ttaaacaaat ctgattcgtt tcacagccct ccttatcata

99960ctatactcct gacaaaatgc caatactgtt atggttttat ccagttttcc cccaaaatcc 100020tctttcactg atctataaac atagaacaaa tttgaatgag cttaaaacaa aacaaaacaa 100080aacaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaga aaagggagtg 100140taagattagg aagaggaagc cccttcacaa ttaacaccct caaattgaca gcacagacaa 100200gccagatggc tgaaagcacg gaatcagaac ccaacttaaa acaggaagta gtgagaatcc 100260caaaggaatt attaaagcat caatatttga acccatatta ttgttagagt tggctcaagt 100320ttatattata tatatatata tatatatata tatatatata tatatatata tatatataaa 100380caaaatcagg gggtcccaat aaataggatt tataacccct gttaagaaat aacccaattt 100440cccaaatttc cctatcataa ctatttatct tctactaaga acagaggcat cactgaacta 100500gcaagggaga gaaataacat ccttgaaata aatgtggtcc atggcatagt tcccttagtt 100560tctcatcaaa cagcttatgt ggaattatgt gaatactgaa atgaatctaa cttctaactt 100620acaaacacaa agataacaca gcttaaataa aatcaactaa ttcattaaag aactatatat 100680actcttagga ttactaaatc aagtactctc cactagtctc ctctttatta ggttcaatgt 100740tcagttgaac agtttccaaa gacattctaa gattccactc tccatgatca cttactatag 100800ggaaatcttg gacaagttac ataacctctc taaacctcaa ttatcttaaa gcagaattaa 100860aaccctagtc tgggttatca taagagtgcc ttatataaaa tatgtttgca aataaatgaa 100920agagattttt catacccaca gcctaacagc caagtctatt tccttgacct tcttgttcat 100980attactttgt ctcataatac agtaaactca caaattcttc caacacccca cttatatccc 101040tcacagtcta cctggttgta tactcagcac ctagtaagag tctttaaaat tgacaggcgc 101100ccaaatttat tgaacgcatt tgtcattata ctagccgtaa tattaaaata attcatgcaa 101160tatgaatttc aaaaaataaa acagaaacat accatatttc gccaaacttc tgaggagctg 101220gtgaactcta aaatcaggct cttagagaga ttttttaacc taaaatataa aacacctcac 101280tcaattcttt taacataaac tggatagtct ttcagaaaaa aaattactca aattcattat 101340aaaataaaag ccaatttctc ttcaataata cttataaaga tccaaaaata tattgaaaga 101400caaattaccc cactcctatt gccaagaaaa gtagcagggt agctctgtag gctgtgagct 101460gaatattttc cgactaggaa agccatgttt cccatcttca tgtacaaaaa gtaatgctcc 101520acaatccctt tcctcaaacc ccttcagata tctacagtaa gtccacttcc aggtaatctc 101580tatatgaaaa acttctgcta tataaaacct agagtagcaa ccatggctaa atcctctgac 101640ctgtcagtct ctcaagagtg ctaagttgtt gaattagaga aaatgtataa aaagaatata 101700gatgctttcc tgacacataa cagggctctg ttactcaact aagtcactcc atgtctatca 101760caattcttta caaataaaca ataacaatgt acttattaca gagcttaaaa caagctctcc 101820tatgatttta aatttgtgtt catagttcat ttataacttg tcttgaatat ttttcagcct 101880ttaatatctt ttaatcttat tttgtcataa ggagaaaacc taatcctatg gattaagcaa 101940ctacaaaata aataaatctg catattattt ataggtaaat aggttggcag gtaggtgagt 102000gagaaggaaa gaaggaaagg caaatggatg ggctgataaa tgatggatgg gtgggtggat 102060ggacagacag attaataaat tgatagatgg actgatataa cactatggtc tccccaaaga 102120taaactttat cacatactag taaatcctgt ggcataacag taggattctc gttccaatga 102180ctcaaatcag aaaagttcaa atcatcctgc caaatataat atattgctca ctcactgcta 102240ccaattccac ctcagggatc tccccacaat ccaacactat ccctaataat tctccagtgg 102300cctttacttg ctttcggtat agcttaattg ctccatcaca atatgtaaaa ctatgtttcc 102360aaaccctgct agtataccct tccttccaga ctagacaaaa cattgagagt atgtccttaa 102420tttttatact aaatgtttta aaaggtttat agaataaaac agcacagaaa aaaaaaaaaa 102480aagaaacttg ggtagctatg taaatgcaag gctaagaaac ttcaatctgt ttcaaaagac 102540tatccatgtc catttcctta taaaccagtc taactcaaag tcacgccgga aaaattagct 102600ttaaaattct tttttgtaat atttcatctt taaagaaaga atccattgct tcttttcccc 102660acatggttac taagattgtt gtgatacaga gtcttgtcat tttacctcat ttctctctta 102720catatttgac agaactatcc tcctagaaat tagaatttgg tcatatccct ctcatattaa 102780aaaaaaatcc ctatgccttc tcaattctgt taacataaat ttcaaacttt tttttacata 102840ttaactgatg tccttgtcat atggtcacat ccagtaatcc aaacattacc catatatgat 102900gacatcctga aatatcaatc tcccttggtt attgtactct atcatatatc agaaatctct 102960atttctggtc ctaataattt tgcttctcag gatattccac aagaaatgtt ttcctgtgct 103020ctgcaatttc catcctctct ttatctataa aagcctattt aaaaaacaaa caaaactatt 103080cttcttaaaa ctttagagca ccccaaatga acaatgacag ttaaggactg agaatgacaa 103140taacactaac aatgaaaaaa atcagaacct tcacatatta ctaataaaag tatttcaaag 103200gaatataaat atatagccac tttgagaaaa gttttaaaat gcctctagtt ttaaatagtt 103260gccatatttc ccagacaact cacacctaga tatataatca agagaatgct atatatttac 103320aacaaaagtt aaatccatat atatgtatgt attatatatt atatatctat tatataatat 103380ataattattt atgtgtgtgt gtatacacac acacacacaa aataccataa caacccaaaa 103440tgaaagcata cagaagccca tatactgatg actggacaga taatcagcta tttaaaaaac 103500aaacaaacaa acaaacaaac aaaaaaccaa aaaaaaaaaa gaaacaaaaa aaaaaacctt 103560gtagagtttc ttttctttta aatttttttc tgtaaattat aaaacacaca gaaaattgct 103620tagcatacag caggccctgg atttcaaact ccagcactta aaaaaaatta attaaaatca 103680ttaaattgaa gcatgtagaa aaaaatcata aaactcttat cattttacca accactcaga 103740attaaagcaa acacaaaaga tgccatttga tatcattatt taaaatctaa ctattccaag 103800tactggttag agatgctaga aacaaaaatg ttcattcact ggtgatacag tatatgttgg 103860caaatcaaat ttacataaaa tttgccgtat ctaatcaagt taaggatata tcctattttc 103920taacaacttc atccctttaa ttttccctaa agaaaacctt ttacaaagga agtataaaaa 103980tgatctgcgc aatataggaa gcaattcaaa atattcataa gcagtgggat acaatactat 104040ttagttatcc atatactata ataccataaa agatatgtgt caacatacac atctcaaaaa 104100cagataaaac acataaaata aaacacataa aataaagaca cataaactga tggcaagtat 104160gtatgaaaca catctataaa ttcttaagca tgatatctca cttattggca aaggtcatat 104220ttaagtacaa aacagaagat attcaagaac ttcaagaaat ctcttacatg gataagaaga 104280gagggtatta ggacttggag gaagagatta agtttccaaa acaaaaatca aaactggcat 104340ttatattcca ggatggtagg taagtagatt attgatatac tattaagtgc tttaaatatt 104400ttataacatg ttttaataag aaacaatctg aatatacaat ttctaagcta aaaacaaaac 104460cctaagacta tggagacttc catgacacaa tcttcccaat ttattaaaag tatgctaata 104520agataaaaat gtatggctct tgagattatc tccatgagta ggataaagcc atataagcct 104580gacactttaa aaaaaaaaaa aaaacactct gggattaaag ttaaaaattg agagacctta 104640atctacctaa ctatcctctg acctccaccc gcatcacagc atggcactct tatgtccaaa 104700tacacattat acaacacata atgataataa tagtaataat aattcaaaag attaagacaa 104760aatataaagg actaaaggac tagaagaaat gttccctggg tttaatgcca agaacccccc 104820ccaaaaaaaa ctattattat aacatgataa ttttaagcgc agaataggtg tctaaataat 104880taagctatta tctcttgacc actaaaaaga cgaattagta tcaattttta aaagaccttc 104940agaaggtctt tgttaattgt ccaggccact atagtactgc ttaattctgt ttacctttaa 105000attctcaagc ctagagaatt atgtattacc atacatacat atttcaagag cctacagcag 105060atatcctaac ccagacctga acaatctaga aagtagttca ttcaatccaa catttacaaa 105120tacctgcatc tgcagaaggg aaaggaaaag gacaaaaggg agagaacagt gagagaggaa 105180agaactggga gacatgaggt tagaaagaga gaaaaaagag aagaggaaag atggaaaaag 105240ggagaagaca aaggatacag aatacaaaat gtgtacacaa acatttactg ttctcccaga 105300ctcttcagta taggaataga catctaattt caactggtta agttatgtgc atatatctgt 105360ttttacgttc acctcttccc tgtgtttcat ttaactcaaa aaaaaaaaaa aaatggagta 105420agagaaaatt tcagaatcag tagaaagatg atccatacaa agttaaatca gaccaaaaca 105480atctttcatt tttaaagtga catgaaacat aatgtaccag ccagtaatta tacatgatgt 105540taagtacaga taaaagacta aaattagctc agttctccta ctatgcttta agagctctta 105600ctttaaaatt atatagccca atctgtagag gaaagtatat tcctccttta agttcctaaa 105660cttgtacata acttcttatt cccatctcca gtctagaatc ctgtacaatg ttatagaatc 105720agtgagaaac agtaacagag aggatggaga atgggacaga atttccaagc ggagaaattt 105780catttctttg aaactaagga tttggatttt cttaatgtat tttctatcag taacacatga 105840tacagagtca ggtcacttca aaagtgagga caaaaacata ttctctaatg agtctttatc 105900tgaaataaga tttatctatt attatacata ctatacaaca ggtactcaaa aaacaacatt 105960ttaagttacc aaacaaaaac acatttagca aaggcaaata ttatctttat ttttagcata 106020ttgtacgctt agcaaatata tctacacaaa ggggcatagt aaatagaaat cttaagggag 106080aaatactaat aaattgaggc tactctactt ccccttagga tgcactaggc attatttttc 106140ttaaaaaaga aaaagaaaaa gaaaaagaaa aagaaaagaa aaaaaaagaa agagggacta 106200tgcatttcat cttttccttt ccaaaaaaat agcagtatga ttcacatctg atactaggta 106260aagtgctgaa acctataatg agaagagtgg tatcaactaa tgaaaacaag gaatgagttt 106320cagaaaagac aagaattcaa ggaaataaca aaatataaag tttgttcaaa ctaaatggta 106380cattacatgg ttggtttctt ttaatttctt ctatattgtt catatcttct atatttaact 106440agaggtatca gcatttattt tatatcacag attataaaac taaattaatt atggccatgg 106500ttccacaatg gtatctctaa gactactttt tcatatcaga aagtccatta gaagttttaa 106560gtgaatagcc atctctttta ttcaaaaaag caaaaaaaca aacaaataaa aaaaacatag 106620gtttttgttt taaaaaaaga aaaattttat cacattaaaa cactgttctg tcgggcagtg 106680gtggcccacg cctttaatcc ctgcacttgg gaagcagagg caggcggatt tctgagtttg 106740aggtctccct ggtctacaga gtgagttcca ggacagccag ggctatacag agaaaccctg 106800tctcaaaaac aacaacaaca acaaaaacaa aaacaaaaaa aacaaaaaac caccgttcag 106860aaaagtacaa cactgacagt atcttcattc atgtcatagc tatgtaattt gtgtgccaaa 106920ctgcttgctt taaatgtcca gaagaacatt ctgaattgaa gatatatgca ttggacacca 106980cacacacaca aaaaaaattg gttcacaccc atagtcccca gcactggagc agtgtaatca 107040gaaggataag taggtgaggt tatcttcaac tactaagaaa ttgagaccag tgtggactac 107100aagatattct gtcttttaaa aaaacatata aaacaaacaa gaactaaaaa gtgtctcagc 107160acttgggccg aagaccaaga attcaagggc agtctgcact acatgtccag atacagtcta 107220tcctaggtta cataccatga tttttatcaa ctactactac tccgccgcca cctccgccgc 107280caccaccacc actgccgcca cagctaccac catccatcca cgcacacacc cacacacata 107340ccccatcacc attcaaacta gatttctaag gctccataac atattttttt caaagtattt 107400ttttcaacct agtgatgtag ctcactgcta ggtagcaagg ccctgtattc tatccccagc 107460tatgtaaaac taaaattgaa ggattttttt taatatctga tttctgtcaa attaatgaga 107520gttgtgctat actgcttaaa tgatgttgac aggtaagaaa gaccaaattc tgaaatgtac 107580tgagctgctg ctaagagtaa caacagatta cacacaaggt tatgtgtaag gaaaatttta 107640tcctgctcat ataaaaagtt cagtacctat atactactac tactactact ctggtttcac 107700tcaaataagg atgccagctt cagcaagatt tacataggat tctccttcat aagcctaatg 107760caatttcttc gtgaggcttc tcctcagatt tgctacaact gaactgcaag aataagctta 107820actatggtac tatggcccct aaaggctacc ctttcccatc attcttataa ctgattcact 107880tgcaattcat ttaattaaaa atcaggtcaa gaagagcaaa tgacaaggaa ttcagctttg 107940caaataactt atcactaaca atttcatgtt aaatgtacat tacttatttc taggttttac 108000ctgaagccac agaagtaata agtgcttcag aacttcagtg gtttaggaaa caaaacaacc 108060taagcttatt gaaatagtta tcaaatctga aactaattca tatattgtaa ttgcggctgt 108120aaaggagata tacccctgca atcccagctc tcaaaagagg tacataccag aaagaccagg 108180aggcttagcc atgttaacag aaaacaatat aaccgtattc catcatcctt tctcctacaa 108240atttcctggg caagttactt tctttataca gtatctatca atgttcaaga agtatcacat 108300atgagcagca taatcctctg taacaagaag gcagtaccac tttaatatga ttaacaaggc 108360cttagaaaga taaatggcct gctcctattt agctcactct ggctcaagac agatgtcagg 108420aaagcagcaa cttctgacaa catgaatatt gcattcttta ttccatttca ataatcctaa 108480gttgttttgt ttcctaaacc actgaaattc tgagccattt acttattctg tggcttcagg 108540taaaacctaa aagtaataag taatgtatat tgaaacctct ttcaaaaatc agtataaaaa 108600atttttttca tgtatagata ggcccaatac ttataatctt tccaaattac tattcattag 108660tttagttgtt atagacaact gaaatcaaga aagtataggt aatattttga aattaaatga 108720aaacaaagga aatctgatgt gtacattaat aagtcacttt attaagaagg aataagtgtc 108780atgtatttca agcctttcat ggttggaaaa agaaattgag aaaaataata atatttctac 108840ttaatacaat ttatttcaca tcttttattt aaaagaatta aatcctgtgc tatcttataa 108900ggattatatt ttactaatag tataaaaata tataactcca taaaagtgaa ttgctaattg 108960caaaataagt gtagctattc ttccaaacaa acatctctga ctcttattat tgaatcagaa 109020tggtaatcat tgtccaaata aatgagtcct aacaggctga gtagcatact agactaaatc 109080tatttcattt tatatatacc acatgtgcat gtgtactgta aaaggatatt gatgggctgc 109140aagtaagaca tgaacacata taccaaggat acaaaaaagg atacaaacag tatcctttac 109200aattgtagct aaggggttaa gggatattaa gtctacagaa ctgccctaaa actggagggg 109260cacagacaat taaattccat aacttaacag aattatcaca ttaaaaaata aaatggtgtt 109320atgtgcaata aataaccaac ctatcagttt ccaaatgata gatattttct ttttttttaa 109380cccttataac ccatgtcagc tcacacaatt caaaggagcc tcagaaacac tacctgatac 109440tgataactat ttcagacata ttcttctatg tgatctgaat ctttgcaccc ccccaaaaaa 109500aaacttcact aaaagcctaa aatcggaggt gatgaaatta caagtgtaat ttttaggagg 109560acctacattg atgaatgaga gcaatgtcct aattttaaaa gaccatttag tcttagctga 109620agcaggtgga tctttgagtt agaggccagc ttcgactatt tacctagctt cagaggctag 109680tgagagacac tgacacaaga aagggaagcg gggaggggaa aaagacaaaa gaaaaaagaa 109740aggaagggga gggaaatata gggaaggctg gagagatgat tcaaaagtta aattaaaagc 109800acttgttgct cttccagagg acctgggttc aatttctaga gcccacatgg caattaacaa 109860ctgtctatat cccagtcaca gcagttccaa cactctcttc tcacccctac agacaccaca 109920ccaatgtgga ctctcatata ataaaatcaa aaaaagaaca aacaaaaaca aacaaacaaa 109980aaccctaata aacagagaca gagatacaga gacatagtga tactggcctg caatcacagc 110040tcctatggaa ggcggcagga ctcccatttc aaggtctgag ctggagtgtg tacagaggac 110100taggaagtgg tagagcagtt cctagaactt gagaggccct aggttcagtc cttagtcctc 110160ctaacactac tcaaatttac aatgaaaatt agttctgatc ttaagatttc tcagtgtcct 110220tgactgtgag atacattttt tggagttggg gggaaggggg gtgttgacca gtctttggta 110280ttttataaat aggccaaaca gacttaagga ttctggaaac atagtctgag gccagcctgg 110340tcaatacagc tagctccagg acagccatgg ctacacaaag aaaccctgcc tcccaaaaat 110400attaattaat taattaatta aagaaaaaag ttttcaatta cacattctat gacatctctg 110460attgataact tttttgagac aaggtctcgc ctcttagtct aggcttgcca caatccttcc 110520ttagcctctc aattgttggt ttacagatgt gagacatcat gcctaacata acattgtctt 110580tatttttatc agtaagaaaa agtaactgtc aaaaccaaaa ctctattatc ctaagccttt 110640ctgaagagaa catcaaaaga cttagaggac tatttttata tttttatatt gtttttccgt 110700atttgaaagt acttaaaaga ccacctctta aaactcatct ccagttgcaa aaatcagaac 110760actgatgttt ttaatagctg catataaata taatataaat ccattaacag taatattatc 110820aataataaca aacaaaccta attagaataa atgataatat accagtaacc attcatttaa 110880ggtgttggtt tttttaaagg acatttgaaa agatcatctt aagctcttga acagaaaaaa 110940aaatagctat ggccaattaa aaagaaaatg ttcataaaaa ttgagagaaa caattattac 111000tgcaattatt tgagggaaga cacaaatgtt taagaaatac aactgaataa aatttacatg 111060tcagagacat tatagatacc taatgaatgc aatgttaaaa aaaaaaaaaa aaaaacgaat 111120caaatagaat gagttttgtc tcaggtagaa atcacatttt tctaatttaa aaccataaaa 111180agggtaaata gccggtcctg gtggtataca tttttaatcg cagaaccctg gagacaaagc 111240caagaggaga gttagacgtc atccagcgat ataccataag accctttctc aaaaaaaaaa 111300aaaaaaaagg gtgatggtgg ctatttcagg gataaaaaag ccacctacaa aacctgcata 111360caaaaggaat tttctaaact caaagttaag ctcctcagtt atatgaagag caacatccaa 111420agaagtatgt gtataccact tcacaacaca ccatgcaagg taaatattga cacaaattat 111480ttcacaacct acattctgat tttgaaaaca aaactacaag tcatctcatc tttattatgt 111540gctgtatctt tcttaaacga tagactgcga attgttgaag ttctcatttt tattacagat 111600tctgatacat ggcttttttg taaagctaag tatttatgga tttacagtca tccttactta 111660ggcttatcag caatacagat gcagtatcca cttccttaaa agtgcagtaa gcagtctatt 111720taagaggaga atttaatact aaagcaattt ctgtcataca taacaagaca aaattttcct 111780tttatctctc ctcaattcta aatttacata atttaccaaa aagatatctt aagagtatta 111840actaatctga ggtgtgtcaa aaacattcgt cgtgtctctt gtccaatagg gctttgatat 111900ttagcttttt aacagctgta tttcttaaag ttcaggccat ctttatttca aacgcacttc 111960cctatccaaa acattttaac ataatctgct gcttctccca aaaccatggg ttatacgaat 112020atccttcacg aagtaagata atggtggata acacatccca tatactattt agttaacatt 112080tccaacagta cactagctat ttaacgcaat cccaaagaga ttttatactg ttctatttta 112140gcatgctcaa aagacataaa aaaaattgac gtaaaattgt ctttagagga ttcttctaaa 112200gctgataaag tcttattaaa attatacttt ctatttttaa ggctaacgca attatagcac 112260attcaactct gattcttcca acaaaaagcc tccgcatcaa aatggggaaa acgtcctttt 112320cattttctca actgaaaagg gggaggggag aagaggagga ggccaactgc ttacaggttt 112380gcattgaaaa ttaacacagc agtttctgag gaatcagtaa ctcacgttca actactgaat 112440cctaccagaa gcagcacacg aataaaacca gagcgcaatc cttgggcttg aggtgccgct 112500tcatccgtct tccttccaat ccacccccaa taccccaacc cactaagcac acatctaaat 112560atgtcttccc caccagagcg cccctttgga ggaaaaggcc tgcctttcgc agcctgaagc 112620cacggctggc tctccgatct ctgcttagat gaggcccccg atctcgacgc cctgcacgtc 112680cgccccaccc cgggcccact cgggtacccg tgggtgacaa aaccctccct cctgcgtcca 112740gccagcactg acagttaagg ctgcagcagc cgcagccacc agggcctagt cctggcaccg 112800gcccctccag accacgcgcc ccaagcactc gctcacggag gcgggtgagg gcccggcgcc 112860taccctgtca gcgcgggccg gagggtgacg aggggaccgg cggtacagac cagagggagc 112920gcgccgggca ggccgcagcc gcagcagcac tgacctgtcc tcgcccccgc gcgtgggctg 112980cggccctcgc agctgcggcc gcctcactgg tccttgtcgc cctcgtccac cgccgccgcc 113040cgccaccgcc tcgttccctt tcgctggcgc caccgccggg tctcatgcgg gacccgcgct 113100tctcctcagg aaaccgaggc cgagaggcca ggcgcctggg caaccttgaa gtcggcgctg 113160aagcgctgct gtcgcgatct gtccacagtg gggggcgagg gcggcgagca actccgaaga 113220gaagcagaag gaggcgccac cgtcagcgac aggtcgacgg aagagaagcg taacgaggcg 113280gtgaacagct gcgtggcccg gtgcgcgagc gagagagcga gcgcaggagg ggcgacgagg 113340tgctggcaaa gacgaaaggc cctgtcgtct cctggagtca cccaaagagg agcgccacca 113400tcttgggaga aatacggatc tcgcgaacgc agcactggac gagaggacta ggtgggcgga 113460ccgagtatcc cgacag 113476419DNAARTIFICIALOligonucleotide motif 4aacttcatca atatttccc 19518DNAARTIFICIALOligonucleotide motif 5acttcatcaa tatttccc 18620DNAARTIFICIALOligonucleotide motif 6caacttcatc aatatttccc 20719DNAARTIFICIALOligonucleotide motif 7caacttcatc aatatttcc 19820DNAARTIFICIALOligonucleotide motif 8ccaacttcat caatatttcc 20920DNAARTIFICIALOligonucleotide motif 9cccaacttca tcaatatttc 201020DNAARTIFICIALOligonucleotide motif 10acccaacttc atcaatattt 201119DNAARTIFICIALOligonucleotide motif 11cccaacttca tcaatattt 191219DNAARTIFICIALOligonucleotide motif 12acccaacttc atcaatatt 191318DNAARTIFICIALOligonucleotide motif 13cccaacttca tcaatatt 181419DNAARTIFICIALOligonucleotide motif 14tacccaactt catcaatat 191518DNAARTIFICIALOligonucleotide motif 15tacccaactt catcaata 181619DNAARTIFICIALOligonucleotide motif 16ttacccaact tcatcaata 191719DNAARTIFICIALOligonucleotide motif 17tttacccaac ttcatcaat 191819DNAARTIFICIALOligonucleotide motif 18atactttacc caacttcat

191918DNAARTIFICIALOligonucleotide motif 19tactttaccc aacttcat 182020DNAARTIFICIALOligonucleotide motif 20ttatacttta cccaacttca 202116DNAARTIFICIALOligonucleotide motif 21tcactgttct gacttt 162220DNAARTIFICIALOligonucleotide motif 22ttcaatctct atctcatcat 202320DNAARTIFICIALOligonucleotide motif 23cttcaatctc tatctcatca 202419DNAARTIFICIALOligonucleotide motif 24ttcaatctct atctcatca 192519DNAARTIFICIALOligonucleotide motif 25cttcaatctc tatctcatc 192619DNAARTIFICIALOligonucleotide motif 26acttcaatct ctatctcat 192720DNAARTIFICIALOligonucleotide motif 27cacttcaatc tctatctcat 202818DNAARTIFICIALOligonucleotide motif 28acttcaatct ctatctca 182919DNAARTIFICIALOligonucleotide motif 29cacttcaatc tctatctca 193019DNAARTIFICIALOligonucleotide motif 30acacttcaat ctctatctc 193120DNAARTIFICIALOligonucleotide motif 31tacacttcaa tctctatctc 203219DNAARTIFICIALOligonucleotide motif 32tacacttcaa tctctatct 193317DNAARTIFICIALOligonucleotide motif 33ctttgtctct ctttact 173418DNAARTIFICIALOligonucleotide motif 34tatacctttc tttaaccc 183516DNAARTIFICIALOligonucleotide motif 35tgtttatacc ctttcc 163616DNAARTIFICIALOligonucleotide motif 36tctcctttat gactcc 163717DNAARTIFICIALOligonucleotide motif 37cttctccttt atgactc 173819DNAARTIFICIALOligonucleotide motif 38ccatttattt ccatttatt 193920DNAARTIFICIALOligonucleotide motif 39ctttccattt atttccattt 204020DNAARTIFICIALOligonucleotide motif 40tctttccatt tatttccatt 204116DNAARTIFICIALOligonucleotide motif 41attacccatc cgttct 164218DNAARTIFICIALOligonucleotide motif 42gcattaggca cattacat 184320DNAARTIFICIALOligonucleotide motif 43attattattt aaccttccta 204420DNAARTIFICIALOligonucleotide motif 44acattattat ttaaccttcc 204519DNAARTIFICIALOligonucleotide motif 45cattattatt taaccttcc 194617DNAARTIFICIALOligonucleotide motif 46cctctgctta taacttt 174717DNAARTIFICIALOligonucleotide motif 47ctactatact ttcctct 174817DNAARTIFICIALOligonucleotide motif 48gttctactat actttcc 174918DNAARTIFICIALOligonucleotide motif 49cacctgataa cagaccct 185016DNAARTIFICIALOligonucleotide motif 50cacctgataa cagacc 165118DNAARTIFICIALOligonucleotide motif 51cccaccaaag gatatatt 185218DNAARTIFICIALOligonucleotide motif 52accagctaca ggaacctc 185319DNAARTIFICIALOligonucleotide motif 53ctatatctca ctcctattt 195418DNAARTIFICIALOligonucleotide motif 54ctatatctca ctcctatt 185520DNAARTIFICIALOligonucleotide motif 55ctactatatc tcactcctat 205619DNAARTIFICIALOligonucleotide motif 56tactatatct cactcctat 195719DNAARTIFICIALOligonucleotide motif 57ctactatatc tcactccta 195818DNAARTIFICIALOligonucleotide motif 58tactatatct cactccta 185918DNAARTIFICIALOligonucleotide motif 59ctactatatc tcactcct 186017DNAARTIFICIALOligonucleotide motif 60ctactatatc tcactcc 176117DNAARTIFICIALOligonucleotide motif 61cctactatat ctcactc 176219DNAARTIFICIALOligonucleotide motif 62ctcctactat atctcactc 196318DNAARTIFICIALOligonucleotide motif 63tcctactata tctcactc 186418DNAARTIFICIALOligonucleotide motif 64ctcctactat atctcact 186519DNAARTIFICIALOligonucleotide motif 65tttcctctcc tactatatc 196616DNAARTIFICIALOligonucleotide motif 66atccatatcc tttcct 166717DNAARTIFICIALOligonucleotide motif 67catccatatc ctttcct 176818DNAARTIFICIALOligonucleotide motif 68atcatccata tcctttcc 186918DNAARTIFICIALOligonucleotide motif 69catcatccat atcctttc 187020DNAARTIFICIALOligonucleotide motif 70tacatcatcc atatcctttc 207118DNAARTIFICIALOligonucleotide motif 71acatcatcca tatccttt 187217DNAARTIFICIALOligonucleotide motif 72catcatccat atccttt 177319DNAARTIFICIALOligonucleotide motif 73tacatcatcc atatccttt 197419DNAARTIFICIALOligonucleotide motif 74atacatcatc catatcctt 197518DNAARTIFICIALOligonucleotide motif 75tacatcatcc atatcctt 187620DNAARTIFICIALOligonucleotide motif 76tatacatcat ccatatcctt 207718DNAARTIFICIALOligonucleotide motif 77atacatcatc catatcct 187820DNAARTIFICIALOligonucleotide motif 78atatacatca tccatatcct 207917DNAARTIFICIALOligonucleotide motif 79tacatcatcc atatcct 178019DNAARTIFICIALOligonucleotide motif 80tatacatcat ccatatcct 198117DNAARTIFICIALOligonucleotide motif 81atacatcatc catatcc 178219DNAARTIFICIALOligonucleotide motif 82atatacatca tccatatcc 198318DNAARTIFICIALOligonucleotide motif 83tatacatcat ccatatcc 188420DNAARTIFICIALOligonucleotide motif 84tatatacatc atccatatcc 208516DNAARTIFICIALOligonucleotide motif 85gcttcatatt tctcca 168617DNAARTIFICIALOligonucleotide motif 86catcttgttc tttacct 178716DNAARTIFICIALOligonucleotide motif 87tatattcacc attgcc 168817DNAARTIFICIALOligonucleotide motif 88ccttatattc accattg 178916DNAARTIFICIALOligonucleotide motif 89cctccttata ttcacc 169016DNAARTIFICIALOligonucleotide motif 90cccttccttt attcaa 169117DNAARTIFICIALOligonucleotide motif 91ccttactgtt aaatcct 179218DNAARTIFICIALOligonucleotide motif 92caggcagata acctccaa 189318DNAARTIFICIALOligonucleotide motif 93cagcaggcag ataacctc 189418DNAARTIFICIALOligonucleotide motif 94cgaatcttga catacagg 189517DNAARTIFICIALOligonucleotide motif 95ctcatacttg ctttaat 179617DNAARTIFICIALOligonucleotide motif 96acatctcata cttgctt 179716DNAARTIFICIALOligonucleotide motif 97acatctcata cttgct 169817DNAARTIFICIALOligonucleotide motif 98tacatctcat acttgct 179918DNAARTIFICIALOligonucleotide motif 99cctacatctc atacttgc 1810017DNAARTIFICIALOligonucleotide motif 100ctacatctca tacttgc 1710116DNAARTIFICIALOligonucleotide motif 101tacatctcat acttgc 1610217DNAARTIFICIALOligonucleotide motif 102cctacatctc atacttg 1710317DNAARTIFICIALOligonucleotide motif 103acctacatct catactt 1710416DNAARTIFICIALOligonucleotide motif 104cctacatctc atactt 1610518DNAARTIFICIALOligonucleotide motif 105tacctacatc tcatactt 1810619DNAARTIFICIALOligonucleotide motif 106ttacctacat ctcatactt 1910716DNAARTIFICIALOligonucleotide motif 107acctacatct catact 1610817DNAARTIFICIALOligonucleotide motif 108tacctacatc tcatact 1710918DNAARTIFICIALOligonucleotide motif 109ttacctacat ctcatact 1811017DNAARTIFICIALOligonucleotide motif 110ttacctacat ctcatac 1711117DNAARTIFICIALOligonucleotide motif 111gttacctaca tctcata 1711216DNAARTIFICIALOligonucleotide motif 112gttacctaca tctcat 1611318DNAARTIFICIALOligonucleotide motif 113atatacccaa aggcacct 1811420DNAARTIFICIALOligonucleotide motif 114tctactcatc ctttaactca 2011517DNAARTIFICIALOligonucleotide motif 115ccttaatctg tatcact 1711616DNAARTIFICIALOligonucleotide motif 116ccatacacag cacata 1611717DNAARTIFICIALOligonucleotide motif 117ctccatacac agcacat 1711817DNAARTIFICIALOligonucleotide motif 118cagaataatt ctcctcc 1711916DNAARTIFICIALOligonucleotide motif 119gtcctacata tatacc 1612016DNAARTIFICIALOligonucleotide motif 120tgcttcctta ctaacc 1612116DNAARTIFICIALOligonucleotide motif 121ccctttgtaa tcatct 1612217DNAARTIFICIALOligonucleotide motif 122tccctttgta atcatct 1712316DNAARTIFICIALOligonucleotide motif 123ctgccatcaa taccat 1612417DNAARTIFICIALOligonucleotide motif 124tcactgccat caatacc 1712519DNAARTIFICIALOligonucleotide motif 125attcttactt tattcctca 1912617DNAARTIFICIALOligonucleotide motif 126tcactttcca gatatca 1712719DNAARTIFICIALOligonucleotide motif 127tccttcaaat tccacatac 1912817DNAARTIFICIALOligonucleotide motif 128acatgtccct ttatatt 1712916DNAARTIFICIALOligonucleotide motif 129acatgtccct ttatat 1613018DNAARTIFICIALOligonucleotide motif 130ccaagaaagg agcaagct 1813118DNAARTIFICIALOligonucleotide motif 131tccaagaaag gagcaagc 1813217DNAARTIFICIALOligonucleotide motif 132ctcatccctc caagaaa 1713316DNAARTIFICIALOligonucleotide motif 133tcatccctcc aagaaa 1613419DNAARTIFICIALOligonucleotide motif 134cacctcccta ttacataaa 1913518DNAARTIFICIALOligonucleotide motif 135cacctcccta ttacataa 1813616DNAARTIFICIALOligonucleotide motif 136cctccctatt acataa 1613719DNAARTIFICIALOligonucleotide motif 137ctaaatcttc caattcata 1913817DNAARTIFICIALOligonucleotide motif 138tatcccttga ttatcct 1713917DNAARTIFICIALOligonucleotide motif 139cctctttgtc aaatact 1714017DNAARTIFICIALOligonucleotide motif 140cagcttattt acctctt 1714119DNAARTIFICIALOligonucleotide motif 141actctttacc tctaacact 1914220DNAARTIFICIALOligonucleotide motif 142ttactcttta cctctaacac 2014319DNAARTIFICIALOligonucleotide motif 143ccaacctaat accttaata 1914419DNAARTIFICIALOligonucleotide motif 144taccaaccta ataccttaa 1914516DNAARTIFICIALOligonucleotide motif 145ccaataccca caaacc 1614617DNAARTIFICIALOligonucleotide motif 146ccattattct actttgt 1714718DNAARTIFICIALOligonucleotide motif 147catttcctta tcttcaca 1814819DNAARTIFICIALOligonucleotide motif 148tcatttcctt atcttcaca 1914918DNAARTIFICIALOligonucleotide motif 149aataattcct catttcct 1815019DNAARTIFICIALOligonucleotide motif 150acaataattc ctcatttcc 19

Claims

1. A method for determining the in vitro efficacy profile of a drug candidate using standardized cell cultures of uniformly distributed differentiated neural cells (NCs) from at least two primate species, wherein the differentiated NC cultures are qualified for high throughput screening, the method comprising the steps of: a) dissociating the differentiated NCs from its support after about 20 days to about 45 days of differentiation and reseeding the differentiated NCs in a high-throughput cell culture format; b) incubating the reseeded NCs in a differentiation medium; c) contacting the reseeded NCs with the drug candidate; and d) determining the in vitro efficacy profile of the drug candidate.

2. The method according to claim 1, wherein the primate species are selected from the group consisting of human (Homo sapiens), Cynomolgus monkey (Macaca fascicularis) and Rhesus monkey (Macaca mulatta).

3. The method according to of any one of claim 1 or 2, wherein the differentiated NCs are derived from induced pluripotent stem cells (iPSCs).

4. The method according to claim 1, wherein the differentiated NCs are uniformly distributed over the cell culture area as assessed by cell nucleus staining.

5. The method according to claim 1, wherein step d) additionally comprises monitoring the cell cultures for signs of toxicity.

6. The method according to claim 1, wherein step d) comprises monitoring the cell cultures for a phenotypic change indicative of the efficacy of the drug candidate.

7. The method according to claim 1, wherein the determined in vitro efficacy profile of the drug candidate is used for inter-species comparison of the efficacy profile of a drug candidate, wherein the cell cultures are produced individually from cells of at least two primate species, wherein essentially the same conditions are applied to the cultures for all primate species and wherein the efficacy profile is determined and compared for all primate species.

8. A method for selecting a drug candidate for further development comprising the steps of: (i) determining the in vitro efficacy profile of the drug candidate for a first and a second species according to the method of any one of claims 1 to 7; and (ii) selecting the drug candidate for further development if the efficacy profile of the drug candidate is favourable.

9. The method according to claim 8, wherein the first species is cynomolgus monkey (Macaca fascicularis) and the second species is human (Homo sapiens).

10. The method according to claim 8, wherein the drug candidate comprises a nucleic acid molecule or targets a specific nucleic acid sequence.

11. The method according to claim 8, wherein the drug candidate comprises at least one nucleic acid molecule such as a RNAi agent or an antisense oligonucleotide.

12. The method according to claim 8, wherein the further development comprises determining the in vivo efficacy and/or toxicity profile of the drug candidate.

13. A method for determining the potential in vivo efficacy of a drug candidate wherein the in vitro efficacy profile of a drug candidate is determined according to any one of claims 1 to 7 and wherein the in vitro efficacy profile is indicative for in vivo efficacy.

14. The method according to claim 13, wherein the in vivo efficacy profile is determined in cynomolgus monkey (Macaca fascicularis).

15. The method according to claim 14, wherein the determined in vitro efficacy profile and/or in vivo efficacy profile of the drug candidate is indicative for in vivo efficacy in human (Homo sapiens).

16. (canceled)