METHODS FOR DIAGNOSING DISEASES ASSOCIATED WITH HUMAN SIGNAL PEPTIDE-CONTAINING MOLECULES

Abstract

The invention provides human signal peptide-containing proteins (HSPP) and polynucleotides which identify and encode HSPP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with expression of HSPP.

Description

CROSS-REFERENCE TO RELATED APPLICATION(S)

[0001] This application is a divisional of U.S. patent application Ser. No. 15/234,915, filed Aug. 11, 2016, which is a divisional of U.S. patent application Ser. No. 14/752,868, filed Jun. 27, 2015, now U.S. Pat. No. 9,512,234, which is a divisional of U.S. patent application Ser. No. 14/248,260, filed Apr. 8, 2014, now U.S. Pat. No. 9,102,745, which is a divisional of U.S. patent application Ser. No. 13/397,592, filed Feb. 15, 2012, now U.S. Pat. No. 8,716,445, which is a divisional of U.S. patent application Ser. No. 12/457,389, filed Jun. 9, 2009, now U.S. Pat. No. 8,153,398, which is a continuation of U.S. patent application Ser. No. 11/905,820, filed Oct. 4, 2007, now abandoned, which is a divisional application of U.S. patent application Ser. No. 10/820,474, filed Apr. 7, 2004, now abandoned, which is a divisional of U.S. patent application Ser. No. 09/720,533, filed Mar. 20, 2001, now abandoned, which is the National Phase of International Patent Application No. PCT/US1999/014484, filed Jun. 25, 1999, which claims the benefit under 35 U.S.C. .sctn. 119(e) to U.S. Provisional Patent Application No. 60/090,762, filed Jun. 26, 1998, U.S. Provisional Patent Application No. 60/094,983, filed Jul. 31, 1998, U.S. Provisional Patent Application No. 60/102,686, filed Oct. 1, 1998, and U.S. Provisional Patent Application No. 60/112,129, filed Dec. 11, 1998. These applications are all incorporated herein by reference in their entirety.

TECHNICAL FIELD

[0002] This invention relates to nucleic acid and amino acid sequences of human signal peptide-containing proteins and to the use of these sequences in the diagnosis, treatment, and prevention of cell proliferative disorders including cancer; inflammation; and cardiovascular, neurological, reproductive, and developmental disorders.

BACKGROUND OF THE INVENTION

[0003] Protein transport is essential for cellular function. Transport of a protein may be mediated by a signal peptide located at the amino terminus of the protein itself. The signal peptide is comprised of about ten to twenty hydrophobic amino acids which target the nascent protein from the ribosome to a particular membrane bound compartment such as the endoplasmic reticulum (ER). Proteins targeted to the ER may either proceed through the secretory pathway or remain in any of the secretory organelles such as the ER, Golgi apparatus, or lysosomes. Proteins that transit through the secretory pathway are either secreted into the extracellular space or retained in the plasma membrane. Secreted proteins are often synthesized as inactive precursors that are activated by post-translational processing events during transit through the secretory pathway. Such events include glycosylation, phosphorylation, proteolysis, and removal of the signal peptide by a signal peptidase. Other events that may occur during protein transport include chaperone-dependent unfolding and folding of the nascent protein and interaction of the protein with a receptor or pore complex. Examples of secreted proteins with amino terminal signal peptides are discussed below and include receptors, extracellular matrix molecules, cytokines, hormones, growth and differentiation factors, neuropeptides, vasomediators, phosphokinases, phosphatases, phospholipases, phosphodiesterases, G and Ras-related proteins, ion channels, transporters/pumps, proteases, and transcription factors. (Reviewed in Alberts, B. et al. (1994) Molecular Biology of The Cell, Garland Publishing, New York, N.Y., pp. 557-560, 582-592.)

[0004] G-protein coupled receptors (GPCRs) comprise a superfamily of integral membrane proteins which transduce extracellular signals. GPCRs include receptors for biogenic amines such as dopamine, epinephrine, histamine, glutamate (metabotropic effect), acetylcholine (muscarinic effect), and serotonin; for lipid mediators of inflammation such as prostaglandins, platelet activating factor, and leukotrienes; for peptide hormones such as calcitonin, C5a anaphylatoxin, follicle stimulating hormone, gonadotropin releasing hormone, neurokinin, oxytocin, and thrombin; and for sensory signal mediators such as retinal photopigments and olfactory stimulatory molecules. The structure of these highly conserved receptors consists of seven hydrophobic transmembrane regions, cysteine disulfide bridges between the second and third extracellular loops, an extracellular N-terminus, and a cytoplasmic C-terminus. The N-terminus interacts with ligands, the disulfide bridges interact with agonists and antagonists, and the large third intracellular loop interacts with G proteins to activate second messengers such as cyclic AMP, phospholipase C, inositol triphosphate, or ion channels. (Reviewed in Watson, S. and Arkinstall, S. (1994) The G-protein Linked Receptor Facts Book, Academic Press, San Diego, Calif., pp. 2-6; and Bolander, F. F. (1994) Molecular Endocrinology, Academic Press, San Diego, Calif., pp. 162-176.)

[0005] Other types of receptors include cell surface antigens identified on leukocytic cells of the immune system. These antigens have been identified using systematic, monoclonal antibody (mAb)-based "shot gun" techniques. These techniques have resulted in the production of hundreds of mAbs directed against unknown cell surface leukocytic antigens. These antigens have been grouped into "clusters of differentiation" based on common immunocytochemical localization patterns in various differentiated and undifferentiated leukocytic cell types. Antigens in a given cluster are presumed to identify a single cell surface protein and are assigned a "CD" number. Some of the genes encoding proteins identified by CD antigens have been isolated and characterized as both transmembrane proteins and cell surface proteins anchored to the plasma membrane via covalent attachment to fatty acid-containing glycolipids such as glycosylphosphatidylinositol (GPI). (Reviewed in Barclay, A. N. et al. (1993) The Leucocyte Antigen Facts Book, Academic Press, San Diego, Calif., pp. 144-145; Noel, L. S. et al. (1998) J. Biol. Chem. 273:3878-3883.)

[0006] Tetraspanins are a superfamily of membrane proteins which facilitate the formation and stability of cell-surface signaling complexes containing lineage-specific proteins, integrins, and other tetraspanins. They are involved in cell activation, proliferation (including cancer), differentiation, adhesion, and motility. These proteins cross the membrane four times, have conserved intracellular--and C-termini and an extracellular, non-conserved hydrophilic domain. Tetraspanins include, e.g., platelet and endothelial cell membrane proteins, leukocyte surface proteins, tissue specific and tumorous antigens, and the retinitis pigmentosa-associated gene peripherin. (Maecker, H. T. et al. (1997) FASEB J. 11:428-442.)

[0007] Matrix proteins (MPs) are transmembrane and extracellular proteins which function in formation, growth, remodeling, and maintenance of tissues and as important mediators and regulators of the inflammatory response. The expression and balance of MPs may be perturbed by biochemical changes that result from congenital, epigenetic, or infectious diseases. In addition, MPs affect leukocyte migration, proliferation, differentiation, and activation in the immune response. MPs are frequently characterized by the presence of one or more domains which may include collagen-like domains, EGF-like domains, immunoglobulin-like domains, and fibronectin-like domains. In addition, some MPs are heavily glycosylated. MPs include extracellular proteins such as fibronectin, collagen, and galectin and cell adhesion receptors such as cell adhesion molecules (CAMs), cadherins, and integrins. (Reviewed in Ayad, S. et al. (1994) The Extracellular Matrix Facts Book, Academic Press, San Diego, Calif., pp. 2-16; Ruoslahti, E. (1997) Kidney Int. 51:1413-1417; Sjaastad, M. D. and Nelson, W. J. (1997) BioEssays 19:47-55.)

[0008] Lectins are proteins characterized by their ability to bind carbohydrates on cell membranes by means of discrete, modular carbohydrate recognition domains, CRDs. (Kishore, U. et al. (1997) Matrix Biol. 15:583-592.) Certain cytokines and membrane-spanning proteins have CRDs which may enhance interactions with extracellular or intracellular ligands, with proteins in secretory pathways, or with molecules in signal transduction pathways. The lipocalin superfamily constitutes a phylogenetically conserved group of more than forty proteins that function by binding to and transporting a variety of physiologically important ligands. (Tanaka, T. et al. (1997) J. Biol. Chem. 272:15789-15795; and van't Hof, W. et al. (1997) J. Biol. Chem. 272:1837-1841.) Selectins are a family of calcium ion-dependent lectins expressed on inflamed vascular endothelium and the surface of some leukocytes. (Rossiter, H. et al. (1997) Mol. Med. Today 3:214-222.)

[0009] Protein kinases regulate many different cell proliferation, differentiation, and signaling processes by adding phosphate groups to proteins. Reversible protein phosphorylation is a key strategy for controlling protein functional activity in eukaryotic cells. The high energy phosphate which drives this activation is generally transferred from adenosine triphosphate molecules (ATP) to a particular protein by protein kinases and removed from that protein by protein phosphatases. Phosphorylation occurs in response to extracellular signals, cell cycle checkpoints, and environmental or nutritional stresses. Protein kinases may be roughly divided into two groups; protein tyrosine kinases (PTKs) which phosphorylate tyrosine residues, and serine/threonine kinases (STKs) which phosphorylate serine or threonine residues. A few protein kinases have dual specificity. A majority of kinases contain a similar 250-300 amino acid catalytic domain. (Hardie, G. and Hanks, S. (1995) The Protein Kinase Facts Book, Vol I, pp. 7-47, Academic Press, San Diego, Calif.)

[0010] Protein phosphatases remove phosphate groups from molecules previously modified by protein kinases thus participating in cell signaling, proliferation, differentiation, contacts, and oncogenesis. Protein phosphorylation is a key strategy used to control protein functional activity in eukaryotic cells. The high energy phosphate is transferred from ATP to a protein by protein kinases and removed by protein phosphatases. There appear to be three, evolutionarily-distinct protein phosphatase gene families: protein phosphatases (PPs); protein tyrosine phosphatases (PTPs); and acid/alkaline phosphatases (APs). PPs dephosphorylate phosphoserine/threonine residues and are an important regulator of many cAMP mediated, hormone responses in cells. PTPs reverse the effects of protein tyrosine kinases and therefore play a significant role in cell cycle and cell signaling processes. Although APs dephosphorylate substrates in vitro, their role in vivo is not well known. (Charbonneau, H. and Tonks, N. K. (1992) Annu. Rev. Cell Biol. 8:463-493.)

[0011] Cyclic nucleotides (cAMP and cGMP) function as intracellular second messengers to transduce a variety of extracellular signals, including hormones, light and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) degrade cyclic nucleotides to their corresponding monophosphates, thereby regulating the intracellular concentrations of cyclic nucleotides and their effects on signal transduction. At least seven families of mammalian PDEs have been identified based on substrate specificity and affinity, sensitivity to cofactors and sensitivity to inhibitory drugs. (Beavo, J. A. (1995) Physiological Reviews 75: 725-748.)

[0012] Phospholipases (PLs) are enzymes that catalyze the removal of fatty acid residues from phosphoglycerides. PLs play an important role in transmembrane signal transduction and are named according to the specific ester bond in phosphoglycerides that is hydrolyzed, i.e., A.sub.1, A.sub.2, C or D. PLA.sub.2 cleaves the ester bond at position 2 of the glycerol moiety of membrane phospholipids giving rise to arachidonic acid. Arachidonic acid is the common precursor to four major classes of eicosanoids, namely prostaglandins, prostacyclins, thromboxanes and leukotrienes. Eicosanoids are signaling molecules involved in the contraction of smooth muscle, platelet aggregation, and pain and inflammatory responses. (Alberts, B. et al. (1994) Molecular Biology of The Cell, Garland Publishing, Inc., New York, N.Y., pp. 85, 211, 239-240, 642-645.)

[0013] The nucleotide cyclases, i.e., adenylate and guanylate cyclase, catalyze the synthesis of the cyclic nucleotides, cAMP and cGMP, from ATP and GTP, respectively. They act in concert with phosphodiesterases, which degrade cAMP and cGMP, to regulate the cellular levels of these molecules and their functions. cAMP and cGMP function as intracellular second messengers to transduce a variety of extracellular signals, e.g., hormones, and light and neurotransmitters. (Stryer, L. (1988) Biochemistry W.H. Freeman and Co., New York, pp. 975-980, 1029-1035.)

[0014] Cytokines are produced in response to cell perturbation. Some cytokines are produced as precursor forms, and some form multimers in order to become active. They are produced in groups and in patterns characteristic of the particular stimulus or disease, and the members of the group interact with one another and other molecules to produce an overall biological response. Interleukins, neurotrophins, growth factors, interferons, and chemokines are all families of cytokines which work in conjunction with cellular receptors to regulate cell proliferation and differentiation and to affect such activities as leukocyte migration and function, hematopoietic cell proliferation, temperature regulation, acute response to infections, tissue remodeling, apoptosis, and cell survival. Studies using antibodies or other drugs that modify the activity of a particular cytokine are used to elucidate the roles of individual cytokines in pathology and physiology.

[0015] Chemokines, in particular, are small chemoattractant cytokines involved in inflammation, leukocyte proliferation and migration, angiogenesis and angiostasis, regulation of hematopoiesis, HIV infectivity, and stimulation of cytokine secretion. Chemokines generally contain 70-100 amino acids and are subdivided into four subfamilies based on the presence of conserved cysteine-based motifs. (Callard, R. and Gearing, A. (1994) The Cytokine Facts Book, Academic Press, New York, N.Y., pp. 181-190, 210-213, 223-227.)

[0016] Growth and differentiation factors are secreted proteins which function in intercellular communication. Some factors require oligomerization or association with MPs for activity. Complex interactions among these factors and their receptors trigger intracellular signal transduction pathways that stimulate or inhibit cell division, cell differentiation, cell signaling, and cell motility. Most growth and differentiation factors act on cells in their local environment (paracrine signaling). There are three broad classes of growth and differentiation factors. The first class includes the large polypeptide growth factors such as epidermal growth factor, fibroblast growth factor, transforming growth factor, insulin-like growth factor, and platelet-derived growth factor. The second class includes the hematopoietic growth factors such as the colony stimulating factors (CSFs). Hematopoietic growth factors stimulate the proliferation and differentiation of blood cells such as B-lymphocytes, T-lymphocytes, erythrocytes, platelets, eosinophils, basophils, neutrophils, macrophages, and their stem cell precursors. The third class includes small peptide factors such as bombesin, vasopressin, oxytocin, endothelin, transferrin, angiotensin II, vasoactive intestinal peptide, and bradykinin which function as hormones to regulate cellular functions other than proliferation.

[0017] Growth and differentiation factors play critical roles in neoplastic transformation of cells in vitro and in tumor progression in vivo. Inappropriate expression of growth factors by tumor cells may contribute to vascularization and metastasis of melanotic tumors. During hematopoiesis, growth factor misregulation can result in anemias, leukemias, and lymphomas. Certain growth factors such as interferon are cytotoxic to tumor cells both in vivo and in vitro. Moreover, some growth factors and growth factor receptors are related both structurally and functionally to oncoproteins. In addition, growth factors affect transcriptional regulation of both proto-oncogenes and oncosuppressor genes. (Reviewed in Pimentel, E. (1994) Handbook of Growth Factors, CRC Press, Ann Arbor, Mich., pp. 1-9.)

[0018] Proteolytic enzymes or proteases either activate or deactivate proteins by hydrolyzing peptide bonds. Proteases are found in the cytosol, in membrane-bound compartments, and in the extracellular space. The major families are the zinc, serine, cysteine, thiol, and carboxyl proteases.

[0019] Zinc proteases, e.g., carboxypeptidase A, have a zinc ion bound to the active site. These proteases recognize C-terminal residues that contain an aromatic or bulky aliphatic side chain, and hydrolyze the peptide bond adjacent to the C-terminal residues. Serine proteases have an active site serine residue and include digestive enzymes, e.g., trypsin and chymotrypsin, components of the complement and blood-clotting cascades, and enzymes that control the degradation and turnover of extracellular matrix (ECM) molecules. Cysteine proteases (e.g. cathepsin) are produced by monocytes, macrophages and other immune cells, and are involved in diverse cellular processes ranging from the processing of precursor proteins to intracellular degradation. Overproduction of these enzymes can cause the tissue destruction associated with rheumatoid arthritis and asthma. Thiol proteases, e.g., papain, contain an active site cysteine and are widely distributed within tissues. Carboxyl proteases, e.g., pepsin, are active only under acidic conditions (pH 2 to 3).

[0020] Guanosine triphosphate-binding proteins (G proteins) can be grouped into two major classes: heterotrimeric G proteins and small G proteins. Heterotrimeric G proteins interact with GPCRs that respond to hormones, growth factors, neuromodulators, or other signaling molecules. The interaction between GPCR and G protein allows the G protein to exchange GTP for guanosine diphosphate (GDP). This exchange activates the G protein, allowing it to dissociate from the receptor and interact with the its cognate second messenger-generating protein, e.g., adenylate cyclase, guanylate cyclase, phospholipase C, or ion channels. The hydrolysis of GTP to GDP by the G protein acts as an on-off switch, terminating the action of the G protein and preparing it to interact with another receptor molecule, thus beginning another round of signal transduction.

[0021] The small G proteins consist of single 21-30 kDa polypeptides. They can be classified into five subfamilies: Ras, Rho, Ran, Rab, and ADP-ribosylation factor. These proteins regulate cell growth, cell cycle control, protein secretion, and intracellular vesicle interaction. In particular, the Ras proteins are essential in transducing signals from receptor tyrosine kinases to serine/threonine kinases which control cell growth and differentiation. Mutant Ras proteins, which bind but can not hydrolyze GTP, are permanently activated and cause continuous cell proliferation or cancer. All five subfamilies share common structural features and four conserved motifs. Most of the membrane-bound G proteins require a carboxy terminal isoprenyl group (CAAX), added posttranslationally, for membrane association and biological activity. The G proteins also have a variable effector region, located between motifs I and II, which is characterized as the interaction site for guanine nucleotide exchange factors or GTPase-activating proteins.

[0022] Eukaryotic cells are bound by a membrane and subdivided into membrane-bound compartments. Membranes are impermeable to many ions and polar molecules, therefore transport of these molecules is mediated by ion channels, ion pumps, transport proteins, or pumps. Symporters and antiporters regulate cytosolic pH by transporting ions and small molecules, e.g., amino acids, glucose, and drugs, across membranes; symporters transport small molecules and ions in the same direction, and antiporters, in the opposite direction. Transporter superfamilies include facilitative transporters and active ATP binding cassette transporters involved in multiple-drug resistance and the targeting of antigenic peptides to MHC Class I molecules. These transporters bind to a specific ion or other molecule and undergo conformational changes in order to transfer the ion or molecule across a membrane. Transport can occur by a passive, concentration-dependent mechanism or can be linked to an energy source such as ATP hydrolysis or an ion gradient.

[0023] Ion channels, ion pumps, and transport proteins mediate the transport of molecules across cellular membranes. Symporters and antiporters regulate cytosolic pH by transporting ions and small molecules such as amino acids, glucose, and drugs. Symporters transport small molecules and ions unidirectionally, and antiporters, bidirectionally. Transporter superfamilies include facilitative transporters and active ATP-binding cassette transporters which are involved in multiple-drug resistance and the targeting of antigenic peptides to MHC Class I molecules. These transporters bind to a specific ion or other molecule and undergo a conformational change in order to transfer the ion or molecule across the membrane. Transport can occur by a passive, concentration-dependent mechanism or can be linked to an energy source such as ATP hydrolysis. (Reviewed in Alberts, B. et al. (1994) Molecular Biology of The Cell, Garland Publishing, New York, N.Y., pp. 523-546.)

[0024] Ion channels are formed by transmembrane proteins which create a lined passageway across the membrane through which water and ions, such as Na.sup.+, K.sup.+, Ca.sup.2+, and Cl--, enter and exit the cell. For example, chloride channels are involved in the regulation of the membrane electric potential as well as absorption and secretion of ions across the membrane. Chloride channels also regulate the internal pH of membrane-bound organelles.

[0025] Ion pumps are ATPases which actively maintain membrane gradients. Ion pumps are classified as P, V, or F according to their structure and function. All have one or more binding sites for ATP in their cytosolic domains. The P-class ion pumps include Ca.sup.2+ ATPase and Na+/K.sup.+ ATPase and function in transporting H.sup.+, Na.sup.+, K.sup.+, and Ca.sup.2+ ions. P-class pumps consist of two y and two y transmembrane subunits. The V- and F-class ion pumps have similar structures and but transport only H.sup.+. F class H.sup.+ pumps mediate transport across the membranes of mitochondria and chloroplasts, while V-class H.sup.+ pumps regulate acidity inside lysosomes, endosomes, and plant vacuoles.

[0026] A family of structurally related intrinsic membrane proteins known as facilitative glucose transporters catalyze the movement of glucose and other selected sugars across the plasma membrane. The proteins in this family contain a highly conserved, large transmembrane domain comprised of 12 -helices, and several weakly conserved, cytoplasmic and exoplasmic domains (Pessin, J. E., and Bell, G. I. (1992) Annu. Rev. Physiol. 54:911-930).

[0027] Amino acid transport is mediated by Na.sup.+ dependent amino acid transporters. These transporters are involved in gastrointestinal and renal uptake of dietary and cellular amino acids and in neuronal reuptake of neurotransmitters. Transport of cationic amino acids is mediated by the system y+ family and the cationic amino acid transporter (CAT) family. Members of the CAT family share a high degree of sequence homology, and each contains 12-14 putative transmembrane domains (Ito, K. and Groudine, M. (1997) J. Biol. Chem. 272:26780-26786).

[0028] Proton-coupled, 12 membrane-spanning domain transporters such as PEPT 1 and PEPT 2 are responsible for gastrointestinal absorption and for renal reabsorbtion of peptides using an electrochemical H.sup.+ gradient as the driving force. A heterodimeric peptide transporter, consisting of TAP 1 and TAP 2, is associated with antigen processing. Peptide antigens are transported across the membrane of the endoplasmic reticulum so they can be presented to the major histocompatibility complex class I molecules. Each TAP protein consists of multiple hydrophobic membrane spanning segments and a highly conserved ATP-binding cassette. (Boll, M. et al. (1996) Proc. Natl. Acad. Sci. 93:284-289.)

[0029] Hormones are secreted molecules that travel through the circulation and bind to specific receptors on the surface of, or within, target cells. Although they have diverse biochemical compositions and mechanisms of action, hormones can be grouped into two categories. One category consists of small lipophilic hormones that diffuse through the plasma membrane of target cells, bind to cytosolic or nuclear receptors, and form a complex that alters gene expression. Examples of these molecules include retinoic acid, thyroxine, and the cholesterol-derived steroid hormones such as progesterone, estrogen, testosterone, cortisol, and aldosterone. The second category consists of hydrophilic hormones that function by binding to cell surface receptors that transduce signals across the plasma membrane. Examples of such hormones include amino acid derivatives such as catecholamines and peptide hormones such as glucagon, insulin, gastrin, secretin, cholecystokinin, adrenocorticotropic hormone, follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, and vasopressin. (See, for example, Lodish et al. (1995) Molecular Cell Biology, Scientific American Books Inc., New York, N.Y., pp. 856-864.)

[0030] Neuropeptides and vasomediators (NP/VM) comprise a large family of endogenous signaling molecules. Included in this family are neuropeptides and neuropeptide hormones such as bombesin, neuropeptide Y, neurotensin, neuromedin N, melanocortins, opioids, galanin, somatostatin, tachykinins, urotensin II and related peptides involved in smooth muscle stimulation, vasopressin, vasoactive intestinal peptide, and circulatory system-borne signaling molecules such as angiotensin, complement, calcitonin, endothelins, formyl-methionyl peptides, glucagon, cholecystokinin and gastrin. NP/VMs can transduce signals directly, modulate the activity or release of other neurotransmitters and hormones, and act as catalytic enzymes in cascades. The effects of NP/VMs range from extremely brief to long-lasting. (Reviewed in Martin, C. R. et al. (1985) Endocrine Physiology, Oxford University Press, New York, N.Y., pp. 57-62.)

[0031] Regulatory molecules turn individual genes or groups of genes on and off in response to various inductive mechanisms of the cell or organism; act as transcription factors by determining whether or not transcription is initiated, enhanced, or repressed; and splice transcripts as dictated in a particular cell or tissue. Although they interact with short stretches of DNA scattered throughout the entire genome, most gene expression is regulated near the site at which transcription starts or within the open reading frame of the gene being expressed. Many of the transcription factors incorporate one of a set of DNA-binding structural motifs, each of which contains either y helices or B sheets and binds to the major groove of DNA. (Pabo, C. O. and R. T. Sauer (1992) Ann. Rev. Biochem. 61:1053-95.) Other domains of transcription factors may form crucial contacts with the DNA. In addition, accessory proteins provide important interactions which may convert a particular protein complex to an activator or a repressor or may prevent binding. (Alberts, B. et al. (1994) Molecular Biology of the Cell, Garland Publishing Co, New York, N.Y. pp. 401-474.)

[0032] The discovery of new human signal peptide-containing proteins and the polynucleotides encoding them satisfies a need in the art by providing new compositions which are useful in the diagnosis, prevention, and treatment of cell proliferative disorders including cancer; inflammation; and cardiovascular, neurological, reproductive, and developmental disorders.

SUMMARY OF THE INVENTION

[0033] The invention features substantially purified polypeptides, proteins with signal peptides, referred to collectively as "HSPP" and individually as "HSPP-1", "HSPP-2", "HSPP-3", "HSPP-4", "HSPP-5", "HSPP-6", "HSPP-7", "HSPP-8", "HSPP-9", "HSPP-10", "HSPP-11", "HSPP-12", "HSPP-13", "HSPP-14", "HSPP-15", "HSPP-16", "HSPP-17", "HSPP-18", "HSPP-19", "HSPP-20", "HSPP-21", "HSPP-22", "HSPP-23", "HSPP-24", "HSPP-25", "HSPP-26", "HSPP-27", "HSPP-28", "HSPP-29", "HSPP-30", "HSPP-31", "HSPP-32", "HSPP-33", "HSPP-34", "HSPP-35", "HSPP-36", "HSPP-37", "HSPP-38", "HSPP-39", "HSPP-40", "HSPP-41", "HSPP-42", "HSPP-43", "HSPP-44", "HSPP-45", "HSPP-46", "HSPP-47", "HSPP-48", "HSPP-49", "HSPP-50", "HSPP-51", "HSPP-52", "HSPP-53", "HSPP-54", "HSPP-55", "HSPP-56", "HSPP-57", "HSPP-58", "HSPP-59", "HSPP-60", "HSPP-61", "HSPP-62", "HSPP-63", "HSPP-64", "HSPP-65", "HSPP-66", "HSPP-67", "HSPP-68", "HSPP-69", "HSPP-70", "HSPP-71", "HSPP-72", "HSPP-73", "HSPP-74", "HSPP-75", HSPP-76'', "HSPP-77", "HSPP-78", "HSPP-79", "HSPP-80", "HSPP-81", "HSPP-82", "HSPP-83", "HSPP-84", "HSPP-85", "HSPP-86", "HSPP-87", "HSPP-88", "HSPP-89", "HSPP-90", "HSPP-91", "HSPP-92", "HSPP-93", "HSPP-94", "HSPP-95", "HSPP-96", "HSPP-97", "HSPP-98", "HSPP-99", "HSPP-100", "HSPP-101", "HSPP-102", "HSPP-103", "HSPP-104", "HSPP-105", "HSPP-106", "HSPP-107", "HSPP-108", "HSPP-109", "HSPP-110", HSPP-111'', "HSPP-112", "HSPP-113", "HSPP-114", "HSPP-115", "HSPP-116", "HSPP-117", "HSPP-118", "HSPP-119", "HSPP-120", "HSPP-121", "HSPP-122", "HSPP-123", "HSPP-124", "HSPP-125", "HSPP-126", "HSPP-127", "HSPP-128", "HSPP-129", "HSPP-130", "HSPP-131", "HSPP-132", "HSPP-133", and "HSPP-134". In one aspect, the invention provides a substantially purified polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO: 28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71, SEQ ID NO:72, SEQ ID NO:73, SEQ ID NO:74, SEQ ID NO:75, SEQ ID NO:76, SEQ ID NO:77, SEQ ID NO:78, SEQ ID NO:79, SEQ ID NO:80, SEQ ID NO:81, SEQ ID NO:82, SEQ ID NO:83, SEQ ID NO:84, SEQ ID NO:85, SEQ ID NO:86, SEQ ID NO:87, SEQ ID NO:88, SEQ ID NO:89, SEQ ID NO:90, SEQ ID NO:91, SEQ ID NO:92, SEQ ID NO:93, SEQ ID NO:94, SEQ ID NO:95, SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:100, SEQ ID NO:101, SEQ ID NO:102, SEQ ID NO:103, SEQ ID NO:104, SEQ ID NO:105, SEQ ID NO:106, SEQ ID NO:107, SEQ ID NO:108, SEQ ID NO:109, SEQ ID NO:110, SEQ ID NO:111, SEQ ID NO:112, SEQ ID NO:113, SEQ ID NO:114, SEQ ID NO:115, SEQ ID NO:116, SEQ ID NO:117, SEQ ID NO:118, SEQ ID NO:119, SEQ ID NO:120, SEQ ID NO:121, SEQ ID NO:122, SEQ ID NO:123, SEQ ID NO:124, SEQ ID NO:125, SEQ ID NO:126, SEQ ID NO:127, SEQ ID NO:128, SEQ ID NO:129, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132, SEQ ID NO:133, SEQ ID NO:134 (SEQ ID NO:1-134), and fragments thereof. The invention further provides a substantially purified variant having at least 90% amino acid identity to at least one of the amino acid sequences selected from the group consisting of SEQ ID NO:1-134, and fragments thereof. The invention also provides an isolated and purified polynucleotide encoding the polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof. The invention also includes an isolated and purified polynucleotide variant having at least 90% polynucleotide sequence identity to the polynucleotide encoding the polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof.

[0034] Additionally, the invention provides an isolated and purified polynucleotide which hybridizes under stringent conditions to the polynucleotide encoding the polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof. The invention also provides an isolated and purified polynucleotide having a sequence which is complementary to the polynucleotide encoding the polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof.

[0035] The invention also provides an isolated and purified polynucleotide comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO:135, SEQ ID NO:136, SEQ ID NO:137, SEQ ID NO:138, SEQ ID NO:139, SEQ ID NO:140, SEQ ID NO:141, SEQ ID NO:142, SEQ ID NO:143, SEQ ID NO:144, SEQ ID NO:145, SEQ ID NO:146, SEQ ID NO:147, SEQ ID NO:148, SEQ ID NO:149, SEQ ID NO:150, SEQ ID NO:151, SEQ ID NO:152, SEQ ID NO:153, SEQ ID NO:154, SEQ ID NO:155, SEQ ID NO:156, SEQ ID NO:157, SEQ ID NO:158, SEQ ID NO:159, SEQ ID NO:160, SEQ ID NO:161, SEQ ID NO:162, SEQ ID NO:163, SEQ ID NO:164, SEQ ID NO:165, SEQ ID NO:166, SEQ ID NO:167, SEQ ID NO:168, SEQ ID NO:169, SEQ ID NO:170, SEQ ID NO:171, SEQ ID NO:172, SEQ ID NO:173, SEQ ID NO:174, SEQ ID NO:175, SEQ ID NO:176, SEQ ID NO:177, SEQ ID NO:178, SEQ ID NO:179, SEQ ID NO:180, SEQ ID NO:181, SEQ ID NO:182, SEQ ID NO:183, SEQ ID NO:184, SEQ ID NO:185, SEQ ID NO:186, SEQ ID NO:187, SEQ ID NO:188, SEQ ID NO:189, SEQ ID NO:190, SEQ ID NO:191, SEQ ID NO:192, SEQ ID NO:193, SEQ ID NO:194, SEQ ID NO:195, SEQ ID NO:196, SEQ ID NO:197, SEQ ID NO:198, SEQ ID NO:199, SEQ ID NO:200, SEQ ID NO:201, SEQ ID NO:202, SEQ ID NO:203, SEQ ID NO:204, SEQ ID NO:205, SEQ ID NO:206, SEQ ID NO:207, SEQ ID NO:208, SEQ ID NO:209, SEQ ID NO:210, SEQ ID NO:211, SEQ ID NO:212, SEQ ID NO:213, SEQ ID NO:214, SEQ ID NO:215, SEQ ID NO:216, SEQ ID NO:217, SEQ ID NO:218, SEQ ID NO:219, SEQ ID NO:220, SEQ ID NO:221, SEQ ID NO:222, SEQ ID NO:223, SEQ ID NO:224, SEQ ID NO:225, SEQ ID NO:226, SEQ ID NO:227, SEQ ID NO:228, SEQ ID NO:229, SEQ ID NO:230, SEQ ID NO:231, SEQ ID NO:232, SEQ ID NO:233, SEQ ID NO:234, SEQ ID NO:235, SEQ ID NO:236, SEQ ID NO:237, SEQ ID NO:238, SEQ ID NO:239, SEQ ID NO:240, SEQ ID NO:241, SEQ ID NO:242, SEQ ID NO:243, SEQ ID NO:244, SEQ ID NO:245, SEQ ID NO:246, SEQ ID NO:247, SEQ ID NO:248, SEQ ID NO:249, SEQ ID NO:250, SEQ ID NO:251, SEQ ID NO:252, SEQ ID NO:253, SEQ ID NO:254, SEQ ID NO:255, SEQ ID NO:256, SEQ ID NO:257, SEQ ID NO:258, SEQ ID NO:259, SEQ ID NO:260, SEQ ID NO:261, SEQ ID NO:262, SEQ ID NO:263, SEQ ID NO:264, SEQ ID NO:265, SEQ ID NO:266, SEQ ID NO:267, SEQ ID NO:268 (SEQ ID NO:135-268), and fragments thereof. The invention further provides an isolated and purified polynucleotide variant having at least 90% polynucleotide sequence identity to the polynucleotide sequence selected from the group consisting of SEQ ID NO:135-268, and fragments thereof. The invention also provides an isolated and purified polynucleotide having a sequence which is complementary to the polynucleotide comprising a polynucleotide sequence selected from the group consisting of SEQ ID NO:135-268, and fragments thereof.

[0036] The invention also provides a method for detecting a polynucleotide in a sample containing nucleic acids, the method comprising the steps of (a) hybridizing the complement of the polynucleotide sequence to at least one of the polynucleotides of the sample, thereby forming a hybridization complex; and (b) detecting the hybridization complex, wherein the presence of the hybridization complex correlates with the presence of a polynucleotide in the sample. In one aspect, the method further comprises amplifying the polynucleotide prior to hybridization.

[0037] The invention further provides an expression vector containing at least a fragment of the polynucleotide encoding the polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof. In another aspect, the expression vector is contained within a host cell.

[0038] The invention also provides a method for producing a polypeptide, the method comprising the steps of: (a) culturing the host cell containing an expression vector containing at least a fragment of a polynucleotide under conditions suitable for the expression of the polypeptide; and (b) recovering the polypeptide from the host cell culture.

[0039] The invention also provides a pharmaceutical composition comprising a substantially purified polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof, in conjunction with a suitable pharmaceutical carrier.

[0040] The invention further includes a purified antibody which binds to a polypeptide selected from the group consisting of SEQ ID NO:1-134, and fragments thereof. The invention also provides a purified agonist and a purified antagonist to the polypeptide.

[0041] The invention also provides a method for treating or preventing a disorder associated with decreased expression or activity of HSPP, the method comprising administering to a subject in need of such treatment an effective amount of a pharmaceutical composition comprising a substantially purified polypeptide having the amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof, in conjunction with a suitable pharmaceutical carrier.

[0042] The invention also provides a method for treating or preventing a disorder associated with increased expression or activity of HSPP, the method comprising administering to a subject in need of such treatment an effective amount of an antagonist of a polypeptide having an amino acid sequence selected from the group consisting of SEQ ID NO:1-134, and fragments thereof.

BRIEF DESCRIPTION OF THE TABLES

[0043] Table 1 shows nucleotide and polypeptide sequence identification numbers (SEQ ID NO), clone identification numbers (clone ID), cDNA libraries, and cDNA fragments used to assemble full-length sequences encoding HSPP.

[0044] Table 2 shows features of each polypeptide sequence, including predicted signal peptide sequences, and methods and algorithms used for identification of HSPP.

[0045] Table 3 shows the tissue-specific expression patterns of each nucleic acid sequence as determined by northern analysis, diseases, disorders, or conditions associated with these tissues, and the vector into which each cDNA was cloned.

[0046] Table 4 describes the tissues used to construct the cDNA libraries from which Incyte cDNA clones encoding HSPP were isolated.

[0047] Table 5 shows the programs, their descriptions, references, and threshold parameters used to analyze HSPP.

[0048] Table 6 shows the regions of the full-length nucleotide sequences of HSPP to which cDNA fragments of Table 1 correspond.

DESCRIPTION OF THE INVENTION

[0049] Before the present proteins, nucleotide sequences, and methods are described, it is understood that this invention is not limited to the particular machines, materials and methods described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

[0050] It must be noted that as used herein and in the appended claims, the singular forms "a," "an," and "the" include plural reference unless the context clearly dictates otherwise. Thus, for example, a reference to "a host cell" includes a plurality of such host cells, and a reference to "an antibody" is a reference to one or more antibodies and equivalents thereof known to those skilled in the art, and so forth.

[0051] Unless defined otherwise, all technical and scientific terms used herein have the same meanings as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any machines, materials, and methods similar or equivalent to those described herein can be used to practice or test the present invention, the preferred machines, materials and methods are now described. All publications mentioned herein are cited for the purpose of describing and disclosing the cell lines, protocols, reagents and vectors which are reported in the publications and which might be used in connection with the invention. Nothing herein is to be construed as an admission that the invention is not entitled to antedate such disclosure by virtue of prior invention.

Definitions

[0052] "HSPP" refers to the amino acid sequences of substantially purified HSPP obtained from any species, particularly a mammalian species, including bovine, ovine, porcine, murine, equine, and preferably the human species, from any source, whether natural, synthetic, semi-synthetic, or recombinant.

[0053] The term "agonist" refers to a molecule which, when bound to HSPP, increases or prolongs the duration of the effect of HSPP. Agonists may include proteins, nucleic acids, carbohydrates, or any other molecules which bind to and modulate the effect of HSPP.

[0054] An "allelic variant" is an alternative form of the gene encoding HSPP. Allelic variants may result from at least one mutation in the nucleic acid sequence and may result in altered mRNAs or in polypeptides whose structure or function may or may not be altered. Any given natural or recombinant gene may have none, one, or many allelic forms. Common mutational changes which give rise to allelic variants are generally ascribed to natural deletions, additions, or substitutions of nucleotides. Each of these types of changes may occur alone, or in combination with the others, one or more times in a given sequence.

[0055] "Altered" nucleic acid sequences encoding HSPP include those sequences with deletions, insertions, or substitutions of different nucleotides, resulting in a polynucleotide the same as HSPP or a polypeptide with at least one functional characteristic of HSPP. Included within this definition are polymorphisms which may or may not be readily detectable using a particular oligonucleotide probe of the polynucleotide encoding HSPP, and improper or unexpected hybridization to allelic variants, with a locus other than the normal chromosomal locus for the polynucleotide sequence encoding HSPP. The encoded protein may also be "altered," and may contain deletions, insertions, or substitutions of amino acid residues which produce a silent change and result in a functionally equivalent HSPP. Deliberate amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues, as long as the biological or immunological activity of HSPP is retained. For example, negatively charged amino acids may include aspartic acid and glutamic acid, positively charged amino acids may include lysine and arginine, and amino acids with uncharged polar head groups having similar hydrophilicity values may include leucine, isoleucine, and valine; glycine and alanine; asparagine and glutamine; serine and threonine; and phenylalanine and tyrosine.

[0056] The terms "amino acid" or "amino acid sequence" refer to an oligopeptide, peptide, polypeptide, or protein sequence, or a fragment of any of these, and to naturally occurring or synthetic molecules. In this context, "fragments," "immunogenic fragments," or "antigenic fragments" refer to fragments of HSPP which are preferably at least 5 to about 15 amino acids in length, most preferably at least 14 amino acids, and which retain some biological activity or immunological activity of HSPP. Where "amino acid sequence" is recited to refer to an amino acid sequence of a naturally occurring protein molecule, "amino acid sequence" and like terms are not meant to limit the amino acid sequence to the complete native amino acid sequence associated with the recited protein molecule.

[0057] "Amplification" relates to the production of additional copies of a nucleic acid sequence. Amplification is generally carried out using polymerase chain reaction (PCR) technologies well known in the art.

[0058] The term "antagonist" refers to a molecule which, when bound to HSPP, decreases the amount or the duration of the effect of the biological or immunological activity of HSPP. Antagonists may include proteins, nucleic acids, carbohydrates, antibodies, or any other molecules which decrease the effect of HSPP.

[0059] The term "antibody" refers to intact molecules as well as to fragments thereof, such as Fab, F(ab').sub.2, and Fv fragments, which are capable of binding the epitopic determinant. Antibodies that bind HSPP polypeptides can be prepared using intact polypeptides or using fragments containing small peptides of interest as the immunizing antigen. The polypeptide or oligopeptide used to immunize an animal (e.g., a mouse, a rat, or a rabbit) can be derived from the translation of RNA, or synthesized chemically, and can be conjugated to a carrier protein if desired. Commonly used carriers that are chemically coupled to peptides include bovine serum albumin, thyroglobulin, and keyhole limpet hemocyanin (KLH). The coupled peptide is then used to immunize the animal.

[0060] The term "antigenic determinant" refers to that fragment of a molecule (i.e., an epitope) that makes contact with a particular antibody. When a protein or a fragment of a protein is used to immunize a host animal, numerous regions of the protein may induce the production of antibodies which bind specifically to antigenic determinants (given regions or three-dimensional structures on the protein). An antigenic determinant may compete with the intact antigen (i.e., the immunogen used to elicit the immune response) for binding to an antibody.

[0061] The term "antisense" refers to any composition containing a nucleic acid sequence which is complementary to the "sense" strand of a specific nucleic acid sequence. Antisense molecules may be produced by any method including synthesis or transcription. Once introduced into a cell, the complementary nucleotides combine with natural sequences produced by the cell to form duplexes and to block either transcription or translation. The designation "negative" can refer to the antisense strand, and the designation "positive" can refer to the sense strand.

[0062] The term "biologically active," refers to a protein having structural, regulatory, or biochemical functions of a naturally occurring molecule. Likewise, "immunologically active" refers to the capability of the natural, recombinant, or synthetic HSPP, or of any oligopeptide thereof, to induce a specific immune response in appropriate animals or cells and to bind with specific antibodies.

[0063] The terms "complementary" or "complementarity" refer to the natural binding of polynucleotides by base pairing. For example, the sequence "5' A-G-T 3'" bonds to the complementary sequence "3' T-C-A 5'." Complementarity between two single-stranded molecules may be "partial," such that only some of the nucleic acids bind, or it may be "complete," such that total complementarity exists between the single stranded molecules. The degree of complementarity between nucleic acid strands has significant effects on the efficiency and strength of the hybridization between the nucleic acid strands. This is of particular importance in amplification reactions, which depend upon binding between nucleic acids strands, and in the design and use of peptide nucleic acid (PNA) molecules.

[0064] A "composition comprising a given polynucleotide sequence" or a "composition comprising a given amino acid sequence" refer broadly to any composition containing the given polynucleotide or amino acid sequence. The composition may comprise a dry formulation or an aqueous solution. Compositions comprising polynucleotide sequences encoding HSPP or fragments of HSPP may be employed as hybridization probes. The probes may be stored in freeze-dried form and may be associated with a stabilizing agent such as a carbohydrate. In hybridizations, the probe may be deployed in an aqueous solution containing salts (e.g., NaCl), detergents (e.g., sodium dodecyl sulfate; SDS), and other components (e.g., Denhardt's solution, dry milk, salmon sperm DNA, etc.).

[0065] "Consensus sequence" refers to a nucleic acid sequence which has been resequenced to resolve uncalled bases, extended using XL-PCR kit (Perkin-Elmer, Norwalk Conn.) in the 5' and/or the 3' direction, and resequenced, or which has been assembled from the overlapping sequences of more than one Incyte Clone using a computer program for fragment assembly, such as the GELVIEW Fragment Assembly system (GCG, Madison Wis.). Some sequences have been both extended and assembled to produce the consensus sequence.

[0066] The term "correlates with expression of a polynucleotide" indicates that the detection of the presence of nucleic acids, the same or related to a nucleic acid sequence encoding HSPP, by northern analysis is indicative of the presence of nucleic acids encoding HSPP in a sample, and thereby correlates with expression of the transcript from the polynucleotide encoding HSPP.

[0067] A "deletion" refers to a change in the amino acid or nucleotide sequence that results in the absence of one or more amino acid residues or nucleotides.

[0068] The term "derivative" refers to the chemical modification of a polypeptide sequence, or a polynucleotide sequence. Chemical modifications of a polynucleotide sequence can include, for example, replacement of hydrogen by an alkyl, acyl, or amino group. A derivative polynucleotide encodes a polypeptide which retains at least one biological or immunological function of the natural molecule. A derivative polypeptide is one modified by glycosylation, pegylation, or any similar process that retains at least one biological or immunological function of the polypeptide from which it was derived.

[0069] The term "similarity" refers to a degree of complementarity. There may be partial similarity or complete similarity. The word "identity" may substitute for the word "similarity." A partially complementary sequence that at least partially inhibits an identical sequence from hybridizing to a target nucleic acid is referred to as "substantially similar." The inhibition of hybridization of the completely complementary sequence to the target sequence may be examined using a hybridization assay (Southern or northern blot, solution hybridization, and the like) under conditions of reduced stringency. A substantially similar sequence or hybridization probe will compete for and inhibit the binding of a completely similar (identical) sequence to the target sequence under conditions of reduced stringency. This is not to say that conditions of reduced stringency are such that non-specific binding is permitted, as reduced stringency conditions require that the binding of two sequences to one another be a specific (i.e., a selective) interaction. The absence of non-specific binding may be tested by the use of a second target sequence which lacks even a partial degree of complementarity (e.g., less than about 30% similarity or identity). In the absence of non-specific binding, the substantially similar sequence or probe will not hybridize to the second non-complementary target sequence.

[0070] The phrases "percent identity" or "% identity" refer to the percentage of sequence similarity found in a comparison of two or more amino acid or nucleic acid sequences. Percent identity can be determined electronically, e.g., by using the MEGALIGN program (DNASTAR, Madison Wis.) which creates alignments between two or more sequences according to methods selected by the user, e.g., the clustal method. (See, e.g., Higgins, D. G. and P. M. Sharp (1988) Gene 73:237-244.) The clustal algorithm groups sequences into clusters by examining the distances between all pairs. The clusters are aligned pairwise and then in groups. The percentage similarity between two amino acid sequences, e.g., sequence A and sequence B, is calculated by dividing the length of sequence A, minus the number of gap residues in sequence A, minus the number of gap residues in sequence B, into the sum of the residue matches between sequence A and sequence B, times one hundred. Gaps of low or of no similarity between the two amino acid sequences are not included in determining percentage similarity. Percent identity between nucleic acid sequences can also be counted or calculated by other methods known in the art, e.g., the Jotun Hein method. (See, e.g., Hein, J. (1990) Methods Enzymol. 183:626-645.) Identity between sequences can also be determined by other methods known in the art, e.g., by varying hybridization conditions.

[0071] "Human artificial chromosomes" (HACs) are linear microchromosomes which may contain DNA sequences of about 6 kb to 10 Mb in size, and which contain all of the elements required for stable mitotic chromosome segregation and maintenance.

[0072] The term "humanized antibody" refers to antibody molecules in which the amino acid sequence in the non-antigen binding regions has been altered so that the antibody more closely resembles a human antibody, and still retains its original binding ability.

[0073] "Hybridization" refers to any process by which a strand of nucleic acid binds with a complementary strand through base pairing.

[0074] The term "hybridization complex" refers to a complex formed between two nucleic acid sequences by virtue of the formation of hydrogen bonds between complementary bases. A hybridization complex may be formed in solution (e.g., Cot or Rot analysis) or formed between one nucleic acid sequence present in solution and another nucleic acid sequence immobilized on a solid support (e.g., paper, membranes, filters, chips, pins or glass slides, or any other appropriate substrate to which cells or their nucleic acids have been fixed).

[0075] The words "insertion" or "addition" refer to changes in an amino acid or nucleotide sequence resulting in the addition of one or more amino acid residues or nucleotides, respectively, to the sequence found in the naturally occurring molecule.

[0076] "Immune response" can refer to conditions associated with inflammation, trauma, immune disorders, or infectious or genetic disease, etc. These conditions can be characterized by expression of various factors, e.g., cytokines, chemokines, and other signaling molecules, which may affect cellular and systemic defense systems.

[0077] The term "microarray" refers to an arrangement of distinct polynucleotides on a substrate.

[0078] The terms "element" or "array element" in a microarray context, refer to hybridizable polynucleotides arranged on the surface of a substrate.

[0079] The term "modulate" refers to a change in the activity of HSPP. For example, modulation may cause an increase or a decrease in protein activity, binding characteristics, or any other biological, functional, or immunological properties of HSPP.

[0080] The phrases "nucleic acid" or "nucleic acid sequence," as used herein, refer to a nucleotide, oligonucleotide, polynucleotide, or any fragment thereof. These phrases also refer to DNA or RNA of genomic or synthetic origin which may be single-stranded or double-stranded and may represent the sense or the antisense strand, to peptide nucleic acid (PNA), or to any DNA-like or RNA-like material. In this context, "fragments" refers to those nucleic acid sequences which, comprise a region of unique polynucleotide sequence that specifically identifies SEQ ID NO:135-268, for example, as distinct from any other sequence in the same genome. For example, a fragment of SEQ ID NO:135-268 is useful in hybridization and amplification technologies and in analogous methods that distinguish SEQ ID NO:135-268 from related polynucleotide sequences. A fragment of SEQ ID NO:135-268 is at least about 15-20 nucleotides in length. The precise length of the fragment of SEQ ID NO:135-268 and the region of SEQ ID NO:135-268 to which the fragment corresponds are routinely determinable by one of ordinary skill in the art based on the intended purpose for the fragment. In some cases, a fragment, when translated, would produce polypeptides retaining some functional characteristic, e.g., antigenicity, or structural domain characteristic, e.g., ATP-binding site, of the full-length polypeptide.

[0081] The terms "operably associated" or "operably linked" refer to functionally related nucleic acid sequences. A promoter is operably associated or operably linked with a coding sequence if the promoter controls the translation of the encoded polypeptide. While operably associated or operably linked nucleic acid sequences can be contiguous and in the same reading frame, certain genetic elements, e.g., repressor genes, are not contiguously linked to the sequence encoding the polypeptide but still bind to operator sequences that control expression of the polypeptide.

[0082] The term "oligonucleotide" refers to a nucleic acid sequence of at least about 6 nucleotides to 60 nucleotides, preferably about 15 to 30 nucleotides, and most preferably about 20 to 25 nucleotides, which can be used in PCR amplification or in a hybridization assay or microarray. "Oligonucleotide" is substantially equivalent to the terms "amplimer," "primer," "oligomer," and "probe," as these terms are commonly defined in the art.

[0083] "Peptide nucleic acid" (PNA) refers to an antisense molecule or anti-gene agent which comprises an oligonucleotide of at least about 5 nucleotides in length linked to a peptide backbone of amino acid residues ending in lysine. The terminal lysine confers solubility to the composition. PNAs preferentially bind complementary single stranded DNA or RNA and stop transcript elongation, and may be pegylated to extend their lifespan in the cell.

[0084] The term "sample" is used in its broadest sense. A sample suspected of containing nucleic acids encoding HSPP, or fragments thereof, or HSPP itself, may comprise a bodily fluid; an extract from a cell, chromosome, organelle, or membrane isolated from a cell; a cell; genomic DNA, RNA, or cDNA, in solution or bound to a substrate; a tissue; a tissue print; etc.

[0085] The terms "specific binding" or "specifically binding" refer to that interaction between a protein or peptide and an agonist, an antibody, or an antagonist. The interaction is dependent upon the presence of a particular structure of the protein, e.g., the antigenic determinant or epitope, recognized by the binding molecule. For example, if an antibody is specific for epitope "A," the presence of a polypeptide containing the epitope A, or the presence of free unlabeled A, in a reaction containing free labeled A and the antibody will reduce the amount of labeled A that binds to the antibody.

[0086] The term "stringent conditions" refers to conditions which permit hybridization between polynucleotides and the claimed polynucleotides. Stringent conditions can be defined by salt concentration, the concentration of organic solvent, e.g., formamide, temperature, and other conditions well known in the art. In particular, stringency can be increased by reducing the concentration of salt, increasing the concentration of formamide, or raising the hybridization temperature.

[0087] The term "substantially purified" refers to nucleic acid or amino acid sequences that are removed from their natural environment and are isolated or separated, and are at least about 60% free, preferably about 75% free, and most preferably about 90% free from other components with which they are naturally associated.

[0088] A "substitution" refers to the replacement of one or more amino acids or nucleotides by different amino acids or nucleotides, respectively.

[0089] "Substrate" refers to any suitable rigid or semi-rigid support including membranes, filters, chips, slides, wafers, fibers, magnetic or nonmagnetic beads, gels, tubing, plates, polymers, microparticles and capillaries. The substrate can have a variety of surface forms, such as wells, trenches, pins, channels and pores, to which polynucleotides or polypeptides are bound.

[0090] "Transformation" describes a process by which exogenous DNA enters and changes a recipient cell. Transformation may occur under natural or artificial conditions according to various methods well known in the art, and may rely on any known method for the insertion of foreign nucleic acid sequences into a prokaryotic or eukaryotic host cell. The method for transformation is selected based on the type of host cell being transformed and may include, but is not limited to, viral infection, electroporation, heat shock, lipofection, and particle bombardment. The term "transformed" cells includes stably transformed cells in which the inserted DNA is capable of replication either as an autonomously replicating plasmid or as part of the host chromosome, as well as transiently transformed cells which express the inserted DNA or RNA for limited periods of time.

[0091] A "variant" of HSPP polypeptides refers to an amino acid sequence that is altered by one or more amino acid residues. The variant may have "conservative" changes, wherein a substituted amino acid has similar structural or chemical properties (e.g., replacement of leucine with isoleucine). More rarely, a variant may have "nonconservative" changes (e.g., replacement of glycine with tryptophan). Analogous minor variations may also include amino acid deletions or insertions, or both. Guidance in determining which amino acid residues may be substituted, inserted, or deleted without abolishing biological or immunological activity may be found using computer programs well known in the art, for example, LASERGENE software (DNASTAR).

[0092] The term "variant," when used in the context of a polynucleotide sequence, may encompass a polynucleotide sequence related to HSPP. This definition may also include, for example, "allelic" (as defined above), "splice," "species," or "polymorphic" variants. A splice variant may have significant identity to a reference molecule, but will generally have a greater or lesser number of polynucleotides due to alternate splicing of exons during mRNA processing. The corresponding polypeptide may possess additional functional domains or an absence of domains. Species variants are polynucleotide sequences that vary from one species to another. The resulting polypeptides generally will have significant amino acid identity relative to each other. A polymorphic variant is a variation in the polynucleotide sequence of a particular gene between individuals of a given species. Polymorphic variants also may encompass "single nucleotide polymorphisms" (SNPs) in which the polynucleotide sequence varies by one base. The presence of SNPs may be indicative of, for example, a certain population, a disease state, or a propensity for a disease state.

THE INVENTION

[0093] The invention is based on the discovery of new human signal peptide-containing proteins (HSPP), the polynucleotides encoding HSPP, and the use of these compositions for the diagnosis, treatment, or prevention of cell proliferative disorders including cancer; inflammation; and cardiovascular, neurological, reproductive, and developmental disorders.

[0094] Table 1 lists the Incyte Clones used to derive full length nucleotide sequences encoding HSPP. Columns 1 and 2 show the sequence identification numbers (SEQ ID NO) of the amino acid and nucleic acid sequences, respectively. Column 3 shows the Clone ID of the Incyte Clone in which nucleic acids encoding each HSPP were identified, and column 4, the cDNA libraries from which these clones were isolated. Column 5 shows Incyte clones, their corresponding cDNA libraries, and shotgun sequences. The clones and shotgun sequences are part of the consensus nucleotide sequence of each HSPP and are useful as fragments in hybridization technologies.

[0095] Table 6 shows the regions of the full-length nucleotide sequences of HSPP to which cDNA fragments of Table 1 correspond. Column 1 lists nucleotide sequence identifiers and column 2 shows the clone ID of the Incyte clone in which nucleic acids encoding each HSPP were identified. Column 3 shows Incyte clones and shotgun sequences which are part of the consensus nucleotide sequence of each HSPP and are useful as fragments in hybridization technologies. Column 4 lists the starting nucleotide position and column 5 the ending nucleotide position of the region of the full-length HSPP to which the cDNA fragment corresponds.

[0096] The columns of Table 2 show various properties of the polypeptides of the invention: column 1 references the SEQ ID NO; column 2 shows the number of amino acid residues in each polypeptide; column 3, potential phosphorylation sites; column 4, potential glycosylation sites; column 5, the amino acid residues comprising signature sequences and motifs; column 6, the identity of each protein; and column 7, analytical methods used to identify each HSPP as a signal peptide-containing protein. Note that in column 5, the first line of each cell lists the amino acid residues comprising predicted signal peptide sequences. Additional identifying motifs or signatures are also listed in column 5. Of particular note is the presence of a glycosyl hydrolase family 9 active site signature in SEQ ID NO:126, a ribosomal protein S18 signature in SEQ ID NO:127, an adrenodoxin family iron-sulfur binding region signature and a cytochrome c family heme-binding site signature in SEQ ID NO:132, and a urotensin II signature sequence in SEQ ID NO:96.

[0097] Using BLAST, SEQ ID NO:68 (HSPP-68) has been identified as a TWIK-related acid-sensitive K.sup.+ channel, and SEQ ID NO:92 (HSPP-92) has been identified as a tyrosine-specific protein phosphatase. The tyrosine-specific protein phosphatases signature in SEQ ID NO:92 (HSPP-92) from about V328 through about F340 (including the putative active site cysteine residue at C330) was identified using BLOCKS and PRINTS. Also of note is the identification of SEQ ID NO:66 (HSPP-66) as a steroid binding protein using BLAST.

[0098] The columns of Table 3 show the tissue-specificity and diseases, disorders, or conditions associated with nucleotide sequences encoding HSPP. The first column of Table 3 lists the nucleotide sequence identifiers. The second column lists tissue categories which express HSPP as a fraction of total tissue categories expressing HSPP. The third column lists the diseases, disorders, or conditions associated with those tissues expressing HSPP. The fourth column lists the vectors used to subclone the cDNA library. Of particular note is the expression of SEQ ID NO:200, SEQ ID NO:203, and SEQ ID NO:225 in lung tissues; the expression of SEQ ID NO:212, SEQ ID NO:216, and SEQ ID NO:220 in reproductive tissues; the expression of SEQ ID NO:223 in cancerous tissues; the expression of SEQ ID NO:232 in gastrointestinal tissue, specifically the small intestine or colon (fifteen out of sixteen (93.8%) cDNA libraries); and the expression of SEQ ID NO:224 in cancerous and proliferating tissues. Also of particular interest is the tissue-specific expression of SEQ ID NO:252 and SEQ ID NO:257. SEQ ID NO:252 is derived from OVARTUT01, an ovarian tumor cDNA library and is exclusively expressed in reproductive tumor tissue. SEQ ID NO:257 is derived from THP1AZT01, a 5-aza-2'-deoxycytidine treated human promonocyte cDNA library and is exclusively expressed in hematopoietic tissue.

[0099] The following fragments of the nucleotide sequences encoding HSPP are useful in hybridization or amplification technologies to identify SEQ ID NO:135-268 and to distinguish between SEQ ID NO:135-268 and related polynucleotide sequences. The useful fragments are the fragment of SEQ ID NO:230 from about nucleotide 75 to about nucleotide 104; the fragment of SEQ ID NO:231 from about nucleotide 210 to about nucleotide 239; the fragment of SEQ ID NO:232 from about nucleotide 157 to about nucleotide 186; the fragment of SEQ ID NO:233 from about nucleotide 268 to about nucleotide 297; the fragment of SEQ ID NO:234 from about nucleotide 160 to about nucleotide 186; the fragment of SEQ ID NO:235 from about nucleotide 201 to about nucleotide 230; the fragment of SEQ ID NO:236 from about nucleotide 165 to about nucleotide 194; the fragment of SEQ ID NO:237 from about nucleotide 366 to about nucleotide 395; the fragment of SEQ ID NO:238 from about nucleotide 714 to about nucleotide 743; the fragment of SEQ ID NO:239 from about nucleotide 1731 to about nucleotide 1760; the fragment of SEQ ID NO:240 from about nucleotide 419 to about nucleotide 448; the fragment of SEQ ID NO:241 from about nucleotide 494 to about nucleotide 523; the fragment of SEQ ID NO:242 from about nucleotide 100 to about nucleotide 129; the fragment of SEQ ID NO:243 from about nucleotide 104 to about nucleotide 133; the fragment of SEQ ID NO:244 from about nucleotide 136 to about nucleotide 165; the fragment of SEQ ID NO:245 from about nucleotide 140 to about nucleotide 169; the fragment of SEQ ID NO:246 from about nucleotide 125 to about nucleotide 154; the fragment of SEQ ID NO:247 from about nucleotide 687 to about nucleotide 758; the fragment of SEQ ID NO:248 from about nucleotide 327 to about nucleotide 398; the fragment of SEQ ID NO:249 from about nucleotide 741 to about nucleotide 785; the fragment of SEQ ID NO:250 from about nucleotide 184 to about nucleotide 255; the fragment of SEQ ID NO:251 from about nucleotide 165 to about nucleotide 242; the fragment of SEQ ID NO:252 from about nucleotide 271 to about nucleotide 342; the fragment of SEQ ID NO:253 from about nucleotide 1081 to about nucleotide 1152; the fragment of SEQ ID NO:254 from about nucleotide 781 to about nucleotide 852; the fragment of SEQ ID NO:255 from about nucleotide 620 to about nucleotide 691; the fragment of SEQ ID NO:256 from about nucleotide 872 to about nucleotide 916; the fragment of SEQ ID NO:257 from about nucleotide 242 to about nucleotide 313; the fragment of SEQ ID NO:258 from about nucleotide 595 to about nucleotide 648; the fragment of SEQ ID NO:259 from about nucleotide 163 to about nucleotide 216; the fragment of SEQ ID NO:260 from about nucleotide 244 to about nucleotide 315; the fragment of SEQ ID NO:261 from about nucleotide 75 to about nucleotide 128; the fragment of SEQ ID NO:262 from about nucleotide 650 to about nucleotide 703; the fragment of SEQ ID NO:263 from about nucleotide 143 to about nucleotide 214; the fragment of SEQ ID NO:264 from about nucleotide 434 to about nucleotide 487; the fragment of SEQ ID NO:265 from about nucleotide 218 to about nucleotide 271; the fragment of SEQ ID NO:266 from about nucleotide 89 to about nucleotide 145; the fragment of SEQ ID NO:267 from about nucleotide 198 to about nucleotide 254; and the fragment of SEQ ID NO:268 from about nucleotide 10 to about nucleotide 54.

[0100] The invention also encompasses HSPP variants. A preferred HSPP variant is one which has at least about 80%, more preferably at least about 90%, and most preferably at least about 95% amino acid sequence identity to the HSPP amino acid sequence, and which contains at least one functional or structural characteristic of HSPP.

[0101] The invention also encompasses polynucleotides which encode HSPP. In a particular embodiment, the invention encompasses a polynucleotide sequence comprising a sequence selected from the group consisting of SEQ ID NO:135-268, which encodes HSPP.

[0102] The invention also encompasses a variant of a polynucleotide sequence encoding HSPP. In particular, such a variant polynucleotide sequence will have at least about 80%, more preferably at least about 90%, and most preferably at least about 95% polynucleotide sequence identity to the polynucleotide sequence encoding HSPP. A particular aspect of the invention encompasses a variant of a polynucleotide sequence comprising a sequence selected from the group consisting of SEQ ID NO:135-268 which has at least about 80%, more preferably at least about 90%, and most preferably at least about 95% polynucleotide sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO:135-268. Any one of the polynucleotide variants described above can encode an amino acid sequence which contains at least one functional or structural characteristic of HSPP.

[0103] It will be appreciated by those skilled in the art that as a result of the degeneracy of the genetic code, a multitude of polynucleotide sequences encoding HSPP, some bearing minimal similarity to the polynucleotide sequences of any known and naturally occurring gene, may be produced. Thus, the invention contemplates each and every possible variation of polynucleotide sequence that could be made by selecting combinations based on possible codon choices. These combinations are made in accordance with the standard triplet genetic code as applied to the polynucleotide sequence of naturally occurring HSPP, and all such variations are to be considered as being specifically disclosed.

[0104] Although nucleotide sequences which encode HSPP and its variants are preferably capable of hybridizing to the nucleotide sequence of the naturally occurring HSPP under appropriately selected conditions of stringency, it may be advantageous to produce nucleotide sequences encoding HSPP or its derivatives possessing a substantially different codon usage, e.g., inclusion of non-naturally occurring codons. Codons may be selected to increase the rate at which expression of the peptide occurs in a particular prokaryotic or eukaryotic host in accordance with the frequency with which particular codons are utilized by the host. Other reasons for substantially altering the nucleotide sequence encoding HSPP and its derivatives without altering the encoded amino acid sequences include the production of RNA transcripts having more desirable properties, such as a greater half-life, than transcripts produced from the naturally occurring sequence.

[0105] The invention also encompasses production of DNA sequences which encode HSPP and HSPP derivatives, or fragments thereof, entirely by synthetic chemistry. After production, the synthetic sequence may be inserted into any of the many available expression vectors and cell systems using reagents well known in the art. Moreover, synthetic chemistry may be used to introduce mutations into a sequence encoding HSPP or any fragment thereof.

[0106] Also encompassed by the invention are polynucleotide sequences that are capable of hybridizing to the claimed polynucleotide sequences, and, in particular, to those shown in SEQ ID NO:135-268 and fragments thereof under various conditions of stringency. (See, e.g., Wahl, G. M. and S. L. Berger (1987) Methods Enzymol. 152:399-407; Kimmel, A. R. (1987) Methods Enzymol. 152:507-511.) For example, stringent salt concentration will ordinarily be less than about 750 mM NaCl and 75 mM trisodium citrate, preferably less than about 500 mM NaCl and 50 mM trisodium citrate, and most preferably less than about 250 mM NaCl and 25 mM trisodium citrate. Low stringency hybridization can be obtained in the absence of organic solvent, e.g., formamide, while high stringency hybridization can be obtained in the presence of at least about 35% formamide, and most preferably at least about 50% formamide. Stringent temperature conditions will ordinarily include temperatures of at least about 30.degree. C., more preferably of at least about 37.degree. C., and most preferably of at least about 42.degree. C. Varying additional parameters, such as hybridization time, the concentration of detergent, e.g., sodium dodecyl sulfate (SDS), and the inclusion or exclusion of carrier DNA, are well known to those skilled in the art. Various levels of stringency are accomplished by combining these various conditions as needed. In a preferred embodiment, hybridization will occur at 30.degree. C. in 750 mM NaCl, 75 mM trisodium citrate, and 1% SDS. In a more preferred embodiment, hybridization will occur at 37.degree. C. in 500 mM NaCl, 50 mM trisodium citrate, 1% SDS, 35% formamide, and 100 .mu.g/ml denatured salmon sperm DNA (ssDNA). In a most preferred embodiment, hybridization will occur at 42.degree. C. in 250 mM NaCl, 25 mM trisodium citrate, 1% SDS, 50% formamide, and 200 .mu.g/ml ssDNA. Useful variations on these conditions will be readily apparent to those skilled in the art.

[0107] The washing steps which follow hybridization can also vary in stringency. Wash stringency conditions can be defined by salt concentration and by temperature. As above, wash stringency can be increased by decreasing salt concentration or by increasing temperature. For example, stringent salt concentration for the wash steps will preferably be less than about 30 mM NaCl and 3 mM trisodium citrate, and most preferably less than about 15 mM NaCl and 1.5 mM trisodium citrate. Stringent temperature conditions for the wash steps will ordinarily include temperature of at least about 25.degree. C., more preferably of at least about 42.degree. C., and most preferably of at least about 68.degree. C. In a preferred embodiment, wash steps will occur at 25.degree. C. in 30 mM NaCl, 3 mM trisodium citrate, and 0.1% SDS. In a more preferred embodiment, wash steps will occur at 42.degree. C. in 15 mM NaCl, 1.5 mM trisodium citrate, and 0.1% SDS. In a most preferred embodiment, wash steps will occur at 68.degree. C. in 15 mM NaCl, 1.5 mM trisodium citrate, and 0.1% SDS. Additional variations on these conditions will be readily apparent to those skilled in the art.

[0108] Methods for DNA sequencing are well known in the art and may be used to practice any of the embodiments of the invention. The methods may employ such enzymes as the Klenow fragment of DNA polymerase I, SEQUENASE (US Biochemical, Cleveland Ohio), Taq polymerase (Perkin-Elmer), thermostable T7 polymerase (Amersham Pharmacia Biotech, Piscataway N.J.), or combinations of polymerases and proofreading exonucleases such as those found in the ELONGASE amplification system (Life Technologies, Gaithersburg Md.). Preferably, sequence preparation is automated with machines such as the Hamilton MICROLAB 2200 (Hamilton, Reno Nev.), Peltier Thermal Cycler 200 (PTC200; MJ Research, Watertown Mass.) and the ABI CATALYST 800 (Perkin-Elmer). Sequencing is then carried out using either ABI 373 or 377 DNA sequencing systems (Perkin-Elmer) or the MEGABACE 1000 DNA sequencing system (Molecular Dynamics, Sunnyvale Calif.). The resulting sequences are analyzed using a variety of algorithms which are well known in the art. (See, e.g., Ausubel, F. M. (1997) Short Protocols in Molecular Biology, John Wiley & Sons, New York N.Y., unit 7.7; Meyers, R. A. (1995) Molecular Biology and Biotechnology, Wiley VCH, New York N.Y., pp. 856-853.)

[0109] The nucleic acid sequences encoding HSPP may be extended utilizing a partial nucleotide sequence and employing various PCR-based methods known in the art to detect upstream sequences, such as promoters and regulatory elements. For example, one method which may be employed, restriction-site PCR, uses universal and nested primers to amplify unknown sequence from genomic DNA within a cloning vector. (See, e.g., Sarkar, G. (1993) PCR Methods Applic. 2:318-322.) Another method, inverse PCR, uses primers that extend in divergent directions to amplify unknown sequence from a circularized template. The template is derived from restriction fragments comprising a known genomic locus and surrounding sequences. (See, e.g., Triglia, T. et al. (1988) Nucleic Acids Res. 16:8186.) A third method, capture PCR, involves PCR amplification of DNA fragments adjacent to known sequences in human and yeast artificial chromosome DNA. (See, e.g., Lagerstrom, M. et al. (1991) PCR Methods Applic. 1:111-119.) In this method, multiple restriction enzyme digestions and ligations may be used to insert an engineered double-stranded sequence into a region of unknown sequence before performing PCR. Other methods which may be used to retrieve unknown sequences are known in the art. (See, e.g., Parker, J. D. et al. (1991) Nucleic Acids Res. 19:3055-306). Additionally, one may use PCR, nested primers, and PROMOTERFINDER libraries (Clontech, Palo Alto Calif.) to walk genomic DNA. This procedure avoids the need to screen libraries and is useful in finding intron/exon junctions. For all PCR-based methods, primers may be designed using commercially available software, such as OLIGO 4.06 Primer Analysis software (National Biosciences, Plymouth MN) or another appropriate program, to be about 22 to 30 nucleotides in length, to have a GC content of about 50% or more, and to anneal to the template at temperatures of about 68.degree. C. to 72.degree. C.

[0110] When screening for full-length cDNAs, it is preferable to use libraries that have been size-selected to include larger cDNAs. In addition, random-primed libraries, which often include sequences containing the 5' regions of genes, are preferable for situations in which an oligo d(T) library does not yield a full-length cDNA. Genomic libraries may be useful for extension of sequence into 5' non-transcribed regulatory regions.

[0111] Capillary electrophoresis systems which are commercially available may be used to analyze the size or confirm the nucleotide sequence of sequencing or PCR products. In particular, capillary sequencing may employ flowable polymers for electrophoretic separation, four different nucleotide-specific, laser-stimulated fluorescent dyes, and a charge coupled device camera for detection of the emitted wavelengths. Output/light intensity may be converted to electrical signal using appropriate software (e.g., GENOTYPER and SEQUENCE NAVIGATOR, Perkin-Elmer), and the entire process from loading of samples to computer analysis and electronic data display may be computer controlled. Capillary electrophoresis is especially preferable for sequencing small DNA fragments which may be present in limited amounts in a particular sample.

[0112] In another embodiment of the invention, polynucleotide sequences or fragments thereof which encode HSPP may be cloned in recombinant DNA molecules that direct expression of HSPP, or fragments or functional equivalents thereof, in appropriate host cells. Due to the inherent degeneracy of the genetic code, other DNA sequences which encode substantially the same or a functionally equivalent amino acid sequence may be produced and used to express HSPP.

[0113] The nucleotide sequences of the present invention can be engineered using methods generally known in the art in order to alter HSPP-encoding sequences for a variety of purposes including, but not limited to, modification of the cloning, processing, and/or expression of the gene product. DNA shuffling by random fragmentation and PCR reassembly of gene fragments and synthetic oligonucleotides may be used to engineer the nucleotide sequences. For example, oligonucleotide-mediated site-directed mutagenesis may be used to introduce mutations that create new restriction sites, alter glycosylation patterns, change codon preference, produce splice variants, and so forth.

[0114] In another embodiment, sequences encoding HSPP may be synthesized, in whole or in part, using chemical methods well known in the art. (See, e.g., Caruthers, M. H. et al. (1980) Nucl. Acids Res. Symp. Ser. 215-223, and Horn, T. et al. (1980) Nucl. Acids Res. Symp. Ser. 225-232.) Alternatively, HSPP itself or a fragment thereof may be synthesized using chemical methods. For example, peptide synthesis can be performed using various solid-phase techniques. (See, e.g., Roberge, J. Y. et al. (1995) Science 269:202-204.) Automated synthesis may be achieved using the ABI 431A Peptide Synthesizer (Perkin-Elmer). Additionally, the amino acid sequence of HSPP, or any part thereof, may be altered during direct synthesis and/or combined with sequences from other proteins, or any part thereof, to produce a variant polypeptide.

[0115] The peptide may be substantially purified by preparative high performance liquid chromatography. (See, e.g, Chiez, R. M. and F.Z. Regnier (1990) Methods Enzymol. 182:392-421.) The composition of the synthetic peptides may be confirmed by amino acid analysis or by sequencing. (See, e.g., Creighton, T. (1984) Proteins, Structures and Molecular Properties, WH Freeman, New York N.Y.)

[0116] In order to express a biologically active HSPP, the nucleotide sequences encoding HSPP or derivatives thereof may be inserted into an appropriate expression vector, i.e., a vector which contains the necessary elements for transcriptional and translational control of the inserted coding sequence in a suitable host. These elements include regulatory sequences, such as enhancers, constitutive and inducible promoters, and 5' and 3' untranslated regions in the vector and in polynucleotide sequences encoding HSPP. Such elements may vary in their strength and specificity. Specific initiation signals may also be used to achieve more efficient translation of sequences encoding HSPP. Such signals include the ATG initiation codon and adjacent sequences, e.g. the Kozak sequence. In cases where sequences encoding HSPP and its initiation codon and upstream regulatory sequences are inserted into the appropriate expression vector, no additional transcriptional or translational control signals may be needed. However, in cases where only coding sequence, or a fragment thereof, is inserted, exogenous translational control signals including an in-frame ATG initiation codon should be provided by the vector. Exogenous translational elements and initiation codons may be of various origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of enhancers appropriate for the particular host cell system used. (See, e.g., Scharf, D. et al. (1994) Results Probl. Cell Differ. 20:125-162.)

[0117] Methods which are well known to those skilled in the art may be used to construct expression vectors containing sequences encoding HSPP and appropriate transcriptional and translational control elements. These methods include in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination. (See, e.g., Sambrook, J. et al. (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press, Plainview N. Y., ch. 4, 8, and 16-17; Ausubel, F. M. et al. (1995) Current Protocols in Molecular Biology, John Wiley & Sons, New York N.Y., ch. 9, 13, and 16.)

[0118] A variety of expression vector/host systems may be utilized to contain and express sequences encoding HSPP. These include, but are not limited to, microorganisms such as bacteria transformed with recombinant bacteriophage, plasmid, or cosmid DNA expression vectors; yeast transformed with yeast expression vectors; insect cell systems infected with viral expression vectors (e.g., baculovirus); plant cell systems transformed with viral expression vectors (e.g., cauliflower mosaic virus, CaMV, or tobacco mosaic virus, TMV) or with bacterial expression vectors (e.g., Ti or pBR322 plasmids); or animal cell systems. The invention is not limited by the host cell employed.

[0119] In bacterial systems, a number of cloning and expression vectors may be selected depending upon the use intended for polynucleotide sequences encoding HSPP. For example, routine cloning, subcloning, and propagation of polynucleotide sequences encoding HSPP can be achieved using a multifunctional E. coli vector such as PBLUESCRIPT (Stratagene, La Jolla Calif.) or pSPORT1 plasmid (Life Technologies). Ligation of sequences encoding HSPP into the vector's multiple cloning site disrupts the lacZ gene, allowing a colorimetric screening procedure for identification of transformed bacteria containing recombinant molecules. In addition, these vectors may be useful for in vitro transcription, dideoxy sequencing, single strand rescue with helper phage, and creation of nested deletions in the cloned sequence. (See, e.g., Van Heeke, G. and S. M. Schuster (1989) J. Biol. Chem. 264:5503-5509.) When large quantities of HSPP are needed, e.g. for the production of antibodies, vectors which direct high level expression of HSPP may be used. For example, vectors containing the strong, inducible T5 or T7 bacteriophage promoter may be used.

[0120] Yeast expression systems may be used for production of HSPP. A number of vectors containing constitutive or inducible promoters, such as alpha factor, alcohol oxidase, and PGH, may be used in the yeast Saccharomyces cerevisiae or Pichia pastoris. In addition, such vectors direct either the secretion or intracellular retention of expressed proteins and enable integration of foreign sequences into the host genome for stable propagation. (See, e.g., Ausubel, 1995, supra; Grant et al. (1987) Methods Enzymol. 153:516-54; and Scorer, C. A. et al. (1994) Bio/Technology 12:181-184.)

[0121] Plant systems may also be used for expression of HSPP. Transcription of sequences encoding HSPP may be driven viral promoters, e.g., the 35S and 19S promoters of CaMV used alone or in combination with the omega leader sequence from TMV (Takamatsu, N. (1987) EMBO J. 6:307-311). Alternatively, plant promoters such as the small subunit of RUBISCO or heat shock promoters may be used. (See, e.g., Coruzzi, G. et al. (1984) EMBO J. 3:1671-1680; Broglie, R. et al. (1984) Science 224:838-843; and Winter, J. et al. (1991) Results Probl. Cell Differ. 17:85-105.) These constructs can be introduced into plant cells by direct DNA transformation or pathogen-mediated transfection. (See, e.g., The McGraw Hill Yearbook of Science and Technology (1992) McGraw Hill, New York N.Y., pp. 191-196.)

[0122] In mammalian cells, a number of viral-based expression systems may be utilized. In cases where an adenovirus is used as an expression vector, sequences encoding HSPP may be ligated into an adenovirus transcription/translation complex consisting of the late promoter and tripartite leader sequence. Insertion in a non-essential E1 or E3 region of the viral genome may be used to obtain infective virus which expresses HSPP in host cells. (See, e.g., Logan, J. and T. Shenk (1984) Proc. Natl. Acad. Sci. 81:3655-3659.) In addition, transcription enhancers, such as the Rous sarcoma virus (RSV) enhancer, may be used to increase expression in mammalian host cells. SV40 or EBV-based vectors may also be used for high-level protein expression.

[0123] Human artificial chromosomes (HACs) may also be employed to deliver larger fragments of DNA than can be contained in and expressed from a plasmid. HACs of about 6 kb to 10 Mb are constructed and delivered via conventional delivery methods (liposomes, polycationic amino polymers, or vesicles) for therapeutic purposes. (See, e.g., Harrington, J. J. et al. (1997) Nat Genet. 15:345-355.)

[0124] For long term production of recombinant proteins in mammalian systems, stable expression of HSPP in cell lines is preferred. For example, sequences encoding HSPP can be transformed into cell lines using expression vectors which may contain viral origins of replication and/or endogenous expression elements and a selectable marker gene on the same or on a separate vector. Following the introduction of the vector, cells may be allowed to grow for about 1 to 2 days in enriched media before being switched to selective media. The purpose of the selectable marker is to confer resistance to a selective agent, and its presence allows growth and recovery of cells which successfully express the introduced sequences. Resistant clones of stably transformed cells may be propagated using tissue culture techniques appropriate to the cell type.

[0125] Any number of selection systems may be used to recover transformed cell lines. These include, but are not limited to, the herpes simplex virus thymidine kinase and adenine phosphoribosyltransferase genes, for use in tk.sup.- or apr.sup.- cells, respectively. (See, e.g., Wigler, M. et al. (1977) Cell 11:223-232; Lowy, I. et al. (1980) Cell 22:817-823.) Also, antimetabolite, antibiotic, or herbicide resistance can be used as the basis for selection. For example, dhfr confers resistance to methotrexate; neo confers resistance to the aminoglycosides, neomycin and G-418; and als or pat confer resistance to chlorsulfuron and phosphinotricin acetyltransferase, respectively. (See, e.g., Wigler, M. et al. (1980) Proc. Natl. Acad. Sci. 77:3567-3570; Colbere-Garapin, F. et al. (1981) J. Mol. Biol. 150:1-14.) Additional selectable genes have been described, e.g., trpB and hisD, which alter cellular requirements for metabolites. (See, e.g., Hartman, S. C. and R. C. Mulligan (1988) Proc. Natl. Acad. Sci. 85:8047-8051.) Visible markers, e.g., anthocyanins, green fluorescent proteins (GFP; Clontech), B glucuronidase and its substrate -glucuronide, or luciferase and its substrate luciferin may be used. These markers can be used not only to identify transformants, but also to quantify the amount of transient or stable protein expression attributable to a specific vector system. (See, e.g., Rhodes, C. A. (1995) Methods Mol. Biol. 55:121-131.)

[0126] Although the presence/absence of marker gene expression suggests that the gene of interest is also present, the presence and expression of the gene may need to be confirmed. For example, if the sequence encoding HSPP is inserted within a marker gene sequence, transformed cells containing sequences encoding HSPP can be identified by the absence of marker gene function. Alternatively, a marker gene can be placed in tandem with a sequence encoding HSPP under the control of a single promoter. Expression of the marker gene in response to induction or selection usually indicates expression of the tandem gene as well.

[0127] In general, host cells that contain the nucleic acid sequence encoding HSPP and that express HSPP may be identified by a variety of procedures known to those of skill in the art. These procedures include, but are not limited to, DNA-DNA or DNA-RNA hybridizations, PCR amplification, and protein bioassay or immunoassay techniques which include membrane, solution, or chip based technologies for the detection and/or quantification of nucleic acid or protein sequences.

[0128] Immunological methods for detecting and measuring the expression of HSPP using either specific polyclonal or monoclonal antibodies are known in the art. Examples of such techniques include enzyme-linked immunosorbent assays (ELISAs), radioimmunoassays (RIAs), and fluorescence activated cell sorting (FACS). A two-site, monoclonal-based immunoassay utilizing monoclonal antibodies reactive to two non-interfering epitopes on HSPP is preferred, but a competitive binding assay may be employed. These and other assays are well known in the art. (See, e.g., Hampton, R. et al. (1990) Serological Methods, a Laboratory Manual, APS Press, St Paul Minn., Sect. IV; Coligan, J. E. et al. (1997) Current Protocols in Immunology, Greene Pub. Associates and Wiley-Interscience, New York N.Y.; and Pound, J. D. (1998) Immunochemical Protocols, Humana Press, Totowa N.J.).

[0129] A wide variety of labels and conjugation techniques are known by those skilled in the art and may be used in various nucleic acid and amino acid assays. Means for producing labeled hybridization or PCR probes for detecting sequences related to polynucleotides encoding HSPP include oligolabeling, nick translation, end-labeling, or PCR amplification using a labeled nucleotide. Alternatively, the sequences encoding HSPP, or any fragments thereof, may be cloned into a vector for the production of an mRNA probe. Such vectors are known in the art, are commercially available, and may be used to synthesize RNA probes in vitro by addition of an appropriate RNA polymerase such as T7, T3, or SP6 and labeled nucleotides. These procedures may be conducted using a variety of commercially available kits, such as those provided by Amersham Pharmacia Biotech, Promega (Madison Wis.), and US Biochemical. Suitable reporter molecules or labels which may be used for ease of detection include radionuclides, enzymes, fluorescent, chemiluminescent, or chromogenic agents, as well as substrates, cofactors, inhibitors, magnetic particles, and the like.

[0130] Host cells transformed with nucleotide sequences encoding HSPP may be cultured under conditions suitable for the expression and recovery of the protein from cell culture. The protein produced by a transformed cell may be secreted or retained intracellularly depending on the sequence and/or the vector used. As will be understood by those of skill in the art, expression vectors containing polynucleotides which encode HSPP may be designed to contain signal sequences which direct secretion of HSPP through a prokaryotic or eukaryotic cell membrane.

[0131] In addition, a host cell strain may be chosen for its ability to modulate expression of the inserted sequences or to process the expressed protein in the desired fashion. Such modifications of the polypeptide include, but are not limited to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation, and acylation. Post-translational processing which cleaves a "prepro" form of the protein may also be used to specify protein targeting, folding, and/or activity. Different host cells which have specific cellular machinery and characteristic mechanisms for post-translational activities (e.g., CHO, HeLa, MDCK, HEK293, and WI38), are available from the American Type Culture Collection (ATCC, Manassas, Va.) and may be chosen to ensure the correct modification and processing of the foreign protein.

[0132] In another embodiment of the invention, natural, modified, or recombinant nucleic acid sequences encoding HSPP may be ligated to a heterologous sequence resulting in translation of a fusion protein in any of the aforementioned host systems. For example, a chimeric HSPP protein containing a heterologous moiety that can be recognized by a commercially available antibody may facilitate the screening of peptide libraries for inhibitors of HSPP activity. Heterologous protein and peptide moieties may also facilitate purification of fusion proteins using commercially available affinity matrices. Such moieties include, but are not limited to, glutathione S-transferase (GST), maltose binding protein (MBP), thioredoxin (Trx), calmodulin binding peptide (CBP), 6-His (SEQ ID NO: 269), FLAG, c-myc, and hemagglutinin (HA). GST, MBP, Trx, CBP, and 6-His (SEQ ID NO: 269) enable purification of their cognate fusion proteins on immobilized glutathione, maltose, phenylarsine oxide, calmodulin, and metal-chelate resins, respectively. FLAG, c-myc, and hemagglutinin (HA) enable immunoaffinity purification of fusion proteins using commercially available monoclonal and polyclonal antibodies that specifically recognize these epitope tags. A fusion protein may also be engineered to contain a proteolytic cleavage site located between the HSPP encoding sequence and the heterologous protein sequence, so that HSPP may be cleaved away from the heterologous moiety following purification. Methods for fusion protein expression and purification are discussed in Ausubel (1995, supra, ch 10). A variety of commercially available kits may also be used to facilitate expression and purification of fusion proteins.

[0133] In a further embodiment of the invention, synthesis of radiolabeled HSPP may be achieved in vitro using the TNT rabbit reticulocyte lysate or wheat germ extract systems (Promega). These systems couple transcription and translation of protein-coding sequences operably associated with the T7, T3, or SP6 promoters. Translation takes place in the presence of a radiolabeled amino acid precursor, preferably .sup.35S-methionine.

[0134] Fragments of HSPP may be produced not only by recombinant production, but also by direct peptide synthesis using solid-phase techniques. (See, e.g., Creighton, supra, pp. 55-60.) Protein synthesis may be performed by manual techniques or by automation. Automated synthesis may be achieved, for example, using the ABI 431A Peptide Synthesizer (Perkin-Elmer). Various fragments of HSPP may be synthesized separately and then combined to produce the full length molecule.

Therapeutics

[0135] Chemical and structural similarity, e.g., in the context of sequences and motifs, exists between regions of HSPP and signal peptide sequences. In addition, chemical and structural similarity, in the context of sequences and motifs, exists between HSPP-66 and prostatic steriod-binding C3 precursor from rat (GI 206453); between HSPP-68 and TWIK-related acid-sensitive K.sup.+ channel from human (GI 2465542); and between HSPP-92 and tyrosine specific protein phosphatases (PROSITE PD0000323). In addition, the expression of HSPP is closely associated with proliferative, cancerous, inflamed, cardiovascular, nervous, reproductive, hematopoietic/immune, and developmental tissue. Therefore, HSPP appears to play a role in cell proliferative disorders including cancer; inflammation; and cardiovascular, neurological, reproductive, and developmental disorders. In the treatment of cell proliferative disorders including cancer; inflammation; and cardiovascular, neurological, reproductive, and developmental disorders associated with increased HSPP expression or activity, it is desirable to decrease the expression or activity of HSPP. In the treatment of the above conditions associated with decreased HSPP expression or activity, it is desirable to increase the expression or activity of HSPP.

[0136] Therefore, in one embodiment, HSPP or a fragment or derivative thereof may be administered to a subject to treat or prevent a disorder associated with decreased expression or activity of HSPP. Examples of such disorders include, but are not limited to, cell proliferative disorders such as actinic keratosis, arteriosclerosis, atherosclerosis, bursitis, cirrhosis, hepatitis, mixed connective tissue disease (MCTD), myelofibrosis, paroxysmal nocturnal hemoglobinuria, polycythemia vera, psoriasis, primary thrombocythemia, and cancers including adenocarcinoma, leukemia, lymphoma, melanoma, myeloma, sarcoma, teratocarcinoma, and, in particular, cancers of the adrenal gland, bladder, bone, bone marrow, brain, breast, cervix, gall bladder, ganglia, gastrointestinal tract, heart, kidney, liver, lung, muscle, ovary, pancreas, parathyroid, penis, prostate, salivary glands, skin, spleen, testis, thymus, thyroid, and uterus; inflammatory disorders, such as acquired immunodeficiency syndrome (AIDS), Addison's disease, adult respiratory distress syndrome, allergies, ankylosing spondylitis, amyloidosis, anemia, asthma, atherosclerosis, autoimmune hemolytic anemia, autoimmune thyroiditis, autoimmune polyenodocrinopathy-candidiasis-ectodermal dystrophy (APECED), bronchitis, cholecystitis, contact dermatitis, Crohn's disease, atopic dermatitis, dermatomyositis, diabetes mellitus, emphysema, episodic lymphopenia with lymphocytotoxins, erythroblastosis fetalis, erythema nodosum, atrophic gastritis, glomerulonephritis, Goodpasture's syndrome, gout, Graves' disease, Hashimoto's thyroiditis, hypereosinophilia, irritable bowel syndrome, multiple sclerosis, myasthenia gravis, myocardial or pericardial inflammation, osteoarthritis, osteoporosis, pancreatitis, polymyositis, psoriasis, Reiter's syndrome, rheumatoid arthritis, scleroderma, Sjogren's syndrome, systemic anaphylaxis, systemic lupus erythematosus, systemic sclerosis, thrombocytopenic purpura, ulcerative colitis, uveitis, Werner syndrome, complications of cancer, hemodialysis, and extracorporeal circulation, viral, bacterial, fungal, parasitic, protozoal, and helminthic infections, and trauma; cardiovascular disorders including disorders of the blood vessels such as arteriovenous fistula, atherosclerosis, hypertension, vasculitis, Raynaud's disease, aneurysms, arterial dissections, varicose veins, thrombophlebitis and phlebothrombosis, and vascular tumors; disorders of the heart such as congestive heart failure, ischemic heart disease, angina pectoris, myocardial infarction, hypertensive heart disease, degenerative valvular heart disease, calcific aortic valve stenosis, congenitally bicuspid aortic valve, mitral annular calcification, mitral valve prolapse, rheumatic fever and rheumatic heart disease, infective endocarditis, nonbacterial thrombotic endocarditis, endocarditis of systemic lupus erythematosus, carcinoid heart disease, cardiomyopathy, myocarditis, pericarditis, neoplastic heart disease, and congenital heart disease; and disorders of the lungs such as congenital lung anomalies, atelectasis, pulmonary congestion and edema, pulmonary embolism, pulmonary hemorrhage, pulmonary infarction, pulmonary hypertension, vascular sclerosis, obstructive pulmonary disease, restrictive pulmonary disease, chronic obstructive pulmonary disease, emphysema, chronic bronchitis, bronchial asthma, bronchiectasis, bacterial pneumonia, viral and mycoplasmal pneumonia, lung abscess, pulmonary tuberculosis, diffuse interstitial diseases, pneumoconioses, sarcoidosis, idiopathic pulmonary fibrosis, desquamative interstitial pneumonitis, hypersensitivity pneumonitis, pulmonary eosinophilia bronchiolitis obliterans-organizing pneumonia, diffuse pulmonary hemorrhage syndromes, Goodpasture's syndromes, idiopathic pulmonary hemosiderosis, pulmonary involvement in collagen-vascular disorders, pulmonary alveolar proteinosis, lung tumors, inflammatory and noninflammatory pleural effusions, pneumothorax, and pleural tumors; neurological disorders such as epilepsy, ischemic cerebrovascular disease, stroke, cerebral neoplasms, Alzheimer's disease, Pick's disease, Huntington's disease, dementia, Parkinson's disease and other extrapyramidal disorders, amyotrophic lateral sclerosis and other motor neuron disorders, progressive neural muscular atrophy, retinitis pigmentosa, hereditary ataxias, multiple sclerosis and other demyelinating diseases, bacterial and viral meningitis, brain abscess, subdural empyema, epidural abscess, suppurative intracranial thrombophlebitis, myelitis and radiculitis, viral central nervous system disease; prion diseases including kuru, Creutzfeldt-Jakob disease, and Gerstmann-Straussler-Scheinker syndrome; fatal familial insomnia, nutritional and metabolic diseases of the nervous system, neurofibromatosis, tuberous sclerosis, cerebelloretinal hemangioblastomatosis, encephalotrigeminal syndrome, mental retardation and other developmental disorders of the central nervous system, cerebral palsy, neuroskeletal disorders, autonomic nervous system disorders, cranial nerve disorders, spinal cord diseases, muscular dystrophy and other neuromuscular disorders, peripheral nervous system disorders, dermatomyositis and polymyositis; inherited, metabolic, endocrine, and toxic myopathies; myasthenia gravis, periodic paralysis; mental disorders including mood, anxiety, and schizophrenic disorders; akathesia, amnesia, catatonia, diabetic neuropathy, tardive dyskinesia, dystonias, paranoid psychoses, postherpetic neuralgia, and Tourette's disorder; reproductive disorders such as disorders of prolactin production; infertility, including tubal disease, ovulatory defects, and endometriosis; disruptions of the estrous cycle, disruptions of the menstrual cycle, polycystic ovary syndrome, ovarian hyperstimulation syndrome, endometrial and ovarian tumors, uterine fibroids, autoimmune disorders, ectopic pregnancies, and teratogenesis; cancer of the breast, fibrocystic breast disease, and galactorrhea; disruptions of spermatogenesis, abnormal sperm physiology, cancer of the testis, cancer of the prostate, benign prostatic hyperplasia, prostatitis, Peyronie's disease, carcinoma of the male breast, and gynecomastia; and developmental disorders, such as renal tubular acidosis, anemia, Cushing's syndrome, achondroplastic dwarfism, Duchenne and Becker muscular dystrophy, epilepsy, gonadal dysgenesis, WAGR syndrome (Wilms' tumor, aniridia, genitourinary abnormalities, and mental retardation), Smith-Magenis syndrome, myelodysplastic syndrome, hereditary mucoepithelial dysplasia, hereditary keratodermas, hereditary neuropathies such as Charcot-Marie-Tooth disease and neurofibromatosis, hypothyroidism, hydrocephalus, seizure disorders such as Syndenham's chorea and cerebral palsy, spina bifida, anencephaly, craniorachischisis, congenital glaucoma, cataract, and sensorineural hearing loss.

[0137] In another embodiment, a vector capable of expressing HSPP or a fragment or derivative thereof may be administered to a subject to treat or prevent a disorder associated with decreased expression or activity of HSPP including, but not limited to, those described above.

[0138] In a further embodiment, a pharmaceutical composition comprising a substantially purified HSPP in conjunction with a suitable pharmaceutical carrier may be administered to a subject to treat or prevent a disorder associated with decreased expression or activity of HSPP including, but not limited to, those provided above.

[0139] In still another embodiment, an agonist which modulates the activity of HSPP may be administered to a subject to treat or prevent a disorder associated with decreased expression or activity of HSPP including, but not limited to, those listed above.

[0140] In a further embodiment, an antagonist of HSPP may be administered to a subject to treat or prevent a disorder associated with increased expression or activity of HSPP. Examples of such disorders include, but are not limited to, those described above. In one aspect, an antibody which specifically binds HSPP may be used directly as an antagonist or indirectly as a targeting or delivery mechanism for bringing a pharmaceutical agent to cells or tissue which express HSPP.

[0141] In an additional embodiment, a vector expressing the complement of the polynucleotide encoding HSPP may be administered to a subject to treat or prevent a disorder associated with increased expression or activity of HSPP including, but not limited to, those described above.

[0142] In other embodiments, any of the proteins, antagonists, antibodies, agonists, complementary sequences, or vectors of the invention may be administered in combination with other appropriate therapeutic agents. Selection of the appropriate agents for use in combination therapy may be made by one of ordinary skill in the art, according to conventional pharmaceutical principles. The combination of therapeutic agents may act synergistically to effect the treatment or prevention of the various disorders described above. Using this approach, one may be able to achieve therapeutic efficacy with lower dosages of each agent, thus reducing the potential for adverse side effects.

[0143] An antagonist of HSPP may be produced using methods which are generally known in the art. In particular, purified HSPP may be used to produce antibodies or to screen libraries of pharmaceutical agents to identify those which specifically bind HSPP. Antibodies to HSPP may also be generated using methods that are well known in the art. Such antibodies may include, but are not limited to, polyclonal, monoclonal, chimeric, and single chain antibodies, Fab fragments, and fragments produced by a Fab expression library. Neutralizing antibodies (i.e., those which inhibit dimer formation) are especially preferred for therapeutic use.

[0144] For the production of antibodies, various hosts including goats, rabbits, rats, mice, humans, and others may be immunized by injection with HSPP or with any fragment or oligopeptide thereof which has immunogenic properties. Depending on the host species, various adjuvants may be used to increase immunological response. Such adjuvants include, but are not limited to, Freund's, mineral gels such as aluminum hydroxide, and surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, KLH, and dinitrophenol. Among adjuvants used in humans, BCG (bacilli Calmette-Guerin) and Corynebacterium parvum are especially preferable.

[0145] It is preferred that the oligopeptides, peptides, or fragments used to induce antibodies to HSPP have an amino acid sequence consisting of at least about 5 amino acids, and, more preferably, of at least about 10 amino acids. It is also preferable that these oligopeptides, peptides, or fragments are identical to a portion of the amino acid sequence of the natural protein and contain the entire amino acid sequence of a small, naturally occurring molecule. Short stretches of HSPP amino acids may be fused with those of another protein, such as KLH, and antibodies to the chimeric molecule may be produced.

[0146] Monoclonal antibodies to HSPP may be prepared using any technique which provides for the production of antibody molecules by continuous cell lines in culture. These include, but are not limited to, the hybridoma technique, the human B-cell hybridoma technique, and the EBV-hybridoma technique. (See, e.g., Kohler, G. et al. (1975) Nature 256:495-497; Kozbor, D. et al. (1985) J. Immunol. Methods 81:31-42; Cote, R. J. et al. (1983) Proc. Natl. Acad. Sci. 80:2026-2030; and Cole, S. P. et al. (1984) Mol. Cell Biol. 62:109-120.)

[0147] In addition, techniques developed for the production of "chimeric antibodies," such as the splicing of mouse antibody genes to human antibody genes to obtain a molecule with appropriate antigen specificity and biological activity, can be used. (See, e.g., Morrison, S. L. et al. (1984) Proc. Natl. Acad. Sci. 81:6851-6855; Neuberger, M. S. et al. (1984) Nature 312:604-608; and Takeda, S. et al. (1985) Nature 314:452-454.) Alternatively, techniques described for the production of single chain antibodies may be adapted, using methods known in the art, to produce HSPP-specific single chain antibodies. Antibodies with related specificity, but of distinct idiotypic composition, may be generated by chain shuffling from random combinatorial immunoglobulin libraries. (See, e.g., Burton D. R. (1991) Proc. Natl. Acad. Sci. 88:10134-10137.)

[0148] Antibodies may also be produced by inducing in vivo production in the lymphocyte population or by screening immunoglobulin libraries or panels of highly specific binding reagents as disclosed in the literature. (See, e.g., Orlandi, R. et al. (1989) Proc. Natl. Acad. Sci. 86: 3833-3837; Winter, G. et al. (1991) Nature 349:293-299.)

[0149] Antibody fragments which contain specific binding sites for HSPP may also be generated. For example, such fragments include, but are not limited to, F(ab')2 fragments produced by pepsin digestion of the antibody molecule and Fab fragments generated by reducing the disulfide bridges of the F(ab')2 fragments. Alternatively, Fab expression libraries may be constructed to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity. (See, e.g., Huse, W. D. et al. (1989) Science 246:1275-1281.)

[0150] Various immunoassays may be used for screening to identify antibodies having the desired specificity. Numerous protocols for competitive binding or immunoradiometric assays using either polyclonal or monoclonal antibodies with established specificities are well known in the art. Such immunoassays typically involve the measurement of complex formation between HSPP and its specific antibody. A two-site, monoclonal-based immunoassay utilizing monoclonal antibodies reactive to two non-interfering HSPP epitopes is preferred, but a competitive binding assay may also be employed (Pound, supra).

[0151] Various methods such as Scatchard analysis in conjunction with radioimmunoassay techniques may be used to assess the affinity of antibodies for HSPP. Affinity is expressed as an association constant, K.sub.a, which is defined as the molar concentration of HSPP-antibody complex divided by the molar concentrations of free antigen and free antibody under equilibrium conditions. The K.sub.a determined for a preparation of polyclonal antibodies, which are heterogeneous in their affinities for multiple HSPP epitopes, represents the average affinity, or avidity, of the antibodies for HSPP. The K.sub.a determined for a preparation of monoclonal antibodies, which are monospecific for a particular HSPP epitope, represents a true measure of affinity. High-affinity antibody preparations with K.sub.a ranging from about 10.sup.9 to 10.sup.12 L/mole are preferred for use in immunoassays in which the HSPP-antibody complex must withstand rigorous manipulations. Low-affinity antibody preparations with K.sub.a ranging from about 10.sup.6 to 10.sup.7 L/mole are preferred for use in immunopurification and similar procedures which ultimately require dissociation of HSPP, preferably in active form, from the antibody (Catty, D. (1988) Antibodies, Volume I: A Practical Approach, IRL Press, Washington, D.C.; Liddell, J. E. and Cryer, A. (1991) A Practical Guide to Monoclonal Antibodies, John Wiley & Sons, New York N.Y.).

[0152] The titer and avidity of polyclonal antibody preparations may be further evaluated to determine the quality and suitability of such preparations for certain downstream applications. For example, a polyclonal antibody preparation containing at least 1-2 mg specific antibody/ml, preferably 5-10 mg specific antibody/ml, is preferred for use in procedures requiring precipitation of HSPP-antibody complexes. Procedures for evaluating antibody specificity, titer, and avidity, and guidelines for antibody quality and usage in various applications, are generally available. (See, e.g., Catty, supra, and Coligan et al. supra.)

[0153] In another embodiment of the invention, the polynucleotides encoding HSPP, or any fragment or complement thereof, may be used for therapeutic purposes. In one aspect, the complement of the polynucleotide encoding HSPP may be used in situations in which it would be desirable to block the transcription of the mRNA. In particular, cells may be transformed with sequences complementary to polynucleotides encoding HSPP. Thus, complementary molecules or fragments may be used to modulate HSPP activity, or to achieve regulation of gene function. Such technology is now well known in the art, and sense or antisense oligonucleotides or larger fragments can be designed from various locations along the coding or control regions of sequences encoding HSPP.

[0154] Expression vectors derived from retroviruses, adenoviruses, or herpes or vaccinia viruses, or from various bacterial plasmids, may be used for delivery of nucleotide sequences to the targeted organ, tissue, or cell population. Methods which are well known to those skilled in the art can be used to construct vectors to express nucleic acid sequences complementary to the polynucleotides encoding HSPP. (See, e.g., Sambrook, supra; Ausubel, 1995, supra.)

[0155] Genes encoding HSPP can be turned off by transforming a cell or tissue with expression vectors which express high levels of a polynucleotide, or fragment thereof, encoding HSPP. Such constructs may be used to introduce untranslatable sense or antisense sequences into a cell. Even in the absence of integration into the DNA, such vectors may continue to transcribe RNA molecules until they are disabled by endogenous nucleases. Transient expression may last for a month or more with a non-replicating vector, and may last even longer if appropriate replication elements are part of the vector system.

[0156] As mentioned above, modifications of gene expression can be obtained by designing complementary sequences or antisense molecules (DNA, RNA, or PNA) to the control, 5', or regulatory regions of the gene encoding HSPP. Oligonucleotides derived from the transcription initiation site, e.g., between about positions -10 and +10 from the start site, are preferred. Similarly, inhibition can be achieved using triple helix base-pairing methodology. Triple helix pairing is useful because it causes inhibition of the ability of the double helix to open sufficiently for the binding of polymerases, transcription factors, or regulatory molecules. Recent therapeutic advances using triplex DNA have been described in the literature. (See, e.g., Gee, J. E. et al. (1994) in Huber, B. E. and B. I. Carr, Molecular and Immunologic Approaches, Futura Publishing, Mt. Kisco N. Y., pp. 163-177.) A complementary sequence or antisense molecule may also be designed to block translation of mRNA by preventing the transcript from binding to ribosomes.

[0157] Ribozymes, enzymatic RNA molecules, may also be used to catalyze the specific cleavage of RNA. The mechanism of ribozyme action involves sequence-specific hybridization of the ribozyme molecule to complementary target RNA, followed by endonucleolytic cleavage. For example, engineered hammerhead motif ribozyme molecules may specifically and efficiently catalyze endonucleolytic cleavage of sequences encoding HSPP.

[0158] Specific ribozyme cleavage sites within any potential RNA target are initially identified by scanning the target molecule for ribozyme cleavage sites, including the following sequences: GUA, GUU, and GUC. Once identified, short RNA sequences of between 15 and 20 ribonucleotides, corresponding to the region of the target gene containing the cleavage site, may be evaluated for secondary structural features which may render the oligonucleotide inoperable. The suitability of candidate targets may also be evaluated by testing accessibility to hybridization with complementary oligonucleotides using ribonuclease protection assays.

[0159] Complementary ribonucleic acid molecules and ribozymes of the invention may be prepared by any method known in the art for the synthesis of nucleic acid molecules. These include techniques for chemically synthesizing oligonucleotides such as solid phase phosphoramidite chemical synthesis. Alternatively, RNA molecules may be generated by in vitro and in vivo transcription of DNA sequences encoding HSPP. Such DNA sequences may be incorporated into a wide variety of vectors with suitable RNA polymerase promoters such as T7 or SP6. Alternatively, these cDNA constructs that synthesize complementary RNA, constitutively or inducibly, can be introduced into cell lines, cells, or tissues.

[0160] RNA molecules may be modified to increase intracellular stability and half-life. Possible modifications include, but are not limited to, the addition of flanking sequences at the 5' and/or 3' ends of the molecule, or the use of phosphorothioate or 2' O-methyl rather than phosphodiesterase linkages within the backbone of the molecule. This concept is inherent in the production of PNAs and can be extended in all of these molecules by the inclusion of nontraditional bases such as inosine, queosine, and wybutosine, as well as acetyl-, methyl-, thio-, and similarly modified forms of adenine, cytidine, guanine, thymine, and uridine which are not as easily recognized by endogenous endonucleases.

[0161] Many methods for introducing vectors into cells or tissues are available and equally suitable for use in vivo, in vitro, and ex vivo. For ex vivo therapy, vectors may be introduced into stem cells taken from the patient and clonally propagated for autologous transplant back into that same patient. Delivery by transfection, by liposome injections, or by polycationic amino polymers may be achieved using methods which are well known in the art. (See, e.g., Goldman, C. K. et al. (1997) Nature Biotechnology 15:462-466.)

[0162] Any of the therapeutic methods described above may be applied to any subject in need of such therapy, including, for example, mammals such as dogs, cats, cows, horses, rabbits, monkeys, and most preferably, humans.

[0163] An additional embodiment of the invention relates to the administration of a pharmaceutical or sterile composition, in conjunction with a pharmaceutically acceptable carrier, for any of the therapeutic effects discussed above. Such pharmaceutical compositions may consist of HSPP, antibodies to HSPP, and mimetics, agonists, antagonists, or inhibitors of HSPP. The compositions may be administered alone or in combination with at least one other agent, such as a stabilizing compound, which may be administered in any sterile, biocompatible pharmaceutical carrier including, but not limited to, saline, buffered saline, dextrose, and water. The compositions may be administered to a patient alone, or in combination with other agents, drugs, or hormones.

[0164] The pharmaceutical compositions utilized in this invention may be administered by any number of routes including, but not limited to, oral, intravenous, intramuscular, intra-arterial, intramedullary, intrathecal, intraventricular, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual, or rectal means.

[0165] In addition to the active ingredients, these pharmaceutical compositions may contain suitable pharmaceutically-acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. Further details on techniques for formulation and administration may be found in the latest edition of Remington's Pharmaceutical Sciences (Maack Publishing, Easton Pa.).

[0166] Pharmaceutical compositions for oral administration can be formulated using pharmaceutically acceptable carriers well known in the art in dosages suitable for oral administration. Such carriers enable the pharmaceutical compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like, for ingestion by the patient.

[0167] Pharmaceutical preparations for oral use can be obtained through combining active compounds with solid excipient and processing the resultant mixture of granules (optionally, after grinding) to obtain tablets or dragee cores. Suitable auxiliaries can be added, if desired. Suitable excipients include carbohydrate or protein fillers, such as sugars, including lactose, sucrose, mannitol, and sorbitol; starch from corn, wheat, rice, potato, or other plants; cellulose, such as methyl cellulose, hydroxypropylmethyl-cellulose, or sodium carboxymethylcellulose; gums, including arabic and tragacanth; and proteins, such as gelatin and collagen. If desired, disintegrating or solubilizing agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, and alginic acid or a salt thereof, such as sodium alginate.

[0168] Dragee cores may be used in conjunction with suitable coatings, such as concentrated sugar solutions, which may also contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for product identification or to characterize the quantity of active compound, i.e., dosage.

[0169] Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a coating, such as glycerol or sorbitol. Push-fit capsules can contain active ingredients mixed with fillers or binders, such as lactose or starches, lubricants, such as talc or magnesium stearate, and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid, or liquid polyethylene glycol with or without stabilizers.

[0170] Pharmaceutical formulations suitable for parenteral administration may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks' solution, Ringer's solution, or physiologically buffered saline. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils, such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate, triglycerides, or liposomes. Non-lipid polycationic amino polymers may also be used for delivery. Optionally, the suspension may also contain suitable stabilizers or agents to increase the solubility of the compounds and allow for the preparation of highly concentrated solutions.

[0171] For topical or nasal administration, penetrants appropriate to the particular barrier to be permeated are used in the formulation. Such penetrants are generally known in the art.

[0172] The pharmaceutical compositions of the present invention may be manufactured in a manner that is known in the art, e.g., by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping, or lyophilizing processes.

[0173] The pharmaceutical composition may be provided as a salt and can be formed with many acids, including but not limited to, hydrochloric, sulfuric, acetic, lactic, tartaric, malic, and succinic acid. Salts tend to be more soluble in aqueous or other protonic solvents than are the corresponding free base forms. In other cases, the preferred preparation may be a lyophilized powder which may contain any or all of the following: 1 mM to 50 mM histidine, 0.1% to 2% sucrose, and 2% to 7% mannitol, at a pH range of 4.5 to 5.5, that is combined with buffer prior to use.

[0174] After pharmaceutical compositions have been prepared, they can be placed in an appropriate container and labeled for treatment of an indicated condition. For administration of HSPP, such labeling would include amount, frequency, and method of administration.

[0175] Pharmaceutical compositions suitable for use in the invention include compositions wherein the active ingredients are contained in an effective amount to achieve the intended purpose. The determination of an effective dose is well within the capability of those skilled in the art.

[0176] For any compound, the therapeutically effective dose can be estimated initially either in cell culture assays, e.g., of neoplastic cells or in animal models such as mice, rats, rabbits, dogs, or pigs. An animal model may also be used to determine the appropriate concentration range and route of administration. Such information can then be used to determine useful doses and routes for administration in humans.

[0177] A therapeutically effective dose refers to that amount of active ingredient, for example HSPP or fragments thereof, antibodies of HSPP, and agonists, antagonists or inhibitors of HSPP, which ameliorates the symptoms or condition. Therapeutic efficacy and toxicity may be determined by standard pharmaceutical procedures in cell cultures or with experimental animals, such as by calculating the ED.sub.50 (the dose therapeutically effective in 50% of the population) or LD.sub.50 (the dose lethal to 50% of the population) statistics. The dose ratio of toxic to therapeutic effects is the therapeutic index, and it can be expressed as the LD.sub.50/ED.sub.50 ratio. Pharmaceutical compositions which exhibit large therapeutic indices are preferred. The data obtained from cell culture assays and animal studies are used to formulate a range of dosage for human use. The dosage contained in such compositions is preferably within a range of circulating concentrations that includes the ED.sub.50 with little or no toxicity. The dosage varies within this range depending upon the dosage form employed, the sensitivity of the patient, and the route of administration.

[0178] The exact dosage will be determined by the practitioner, in light of factors related to the subject requiring treatment. Dosage and administration are adjusted to provide sufficient levels of the active moiety or to maintain the desired effect. Factors which may be taken into account include the severity of the disease state, the general health of the subject, the age, weight, and gender of the subject, time and frequency of administration, drug combination(s), reaction sensitivities, and response to therapy. Long-acting pharmaceutical compositions may be administered every 3 to 4 days, every week, or biweekly depending on the half-life and clearance rate of the particular formulation.

[0179] Normal dosage amounts may vary from about 0.1 .mu.g to 100,000 .mu.g, up to a total dose of about 1 gram, depending upon the route of administration. Guidance as to particular dosages and methods of delivery is provided in the literature and generally available to practitioners in the art. Those skilled in the art will employ different formulations for nucleotides than for proteins or their inhibitors. Similarly, delivery of polynucleotides or polypeptides will be specific to particular cells, conditions, locations, etc.

Diagnostics

[0180] In another embodiment, antibodies which specifically bind HSPP may be used for the diagnosis of disorders characterized by expression of HSPP, or in assays to monitor patients being treated with HSPP or agonists, antagonists, or inhibitors of HSPP. Antibodies useful for diagnostic purposes may be prepared in the same manner as described above for therapeutics. Diagnostic assays for HSPP include methods which utilize the antibody and a label to detect HSPP in human body fluids or in extracts of cells or tissues. The antibodies may be used with or without modification, and may be labeled by covalent or non-covalent attachment of a reporter molecule. A wide variety of reporter molecules, several of which are described above, are known in the art and may be used.

[0181] A variety of protocols for measuring HSPP, including ELISAs, RIAs, and FACS, are known in the art and provide a basis for diagnosing altered or abnormal levels of HSPP expression. Normal or standard values for HSPP expression are established by combining body fluids or cell extracts taken from normal mammalian subjects, preferably human, with antibody to HSPP under conditions suitable for complex formation. The amount of standard complex formation may be quantitated by various methods, preferably by photometric means. Quantities of HSPP expressed in subject, control, and disease samples from biopsied tissues are compared with the standard values. Deviation between standard and subject values establishes the parameters for diagnosing disease.

[0182] In another embodiment of the invention, the polynucleotides encoding HSPP may be used for diagnostic purposes. The polynucleotides which may be used include oligonucleotide sequences, complementary RNA and DNA molecules, and PNAs. The polynucleotides may be used to detect and quantitate gene expression in biopsied tissues in which expression of HSPP may be correlated with disease. The diagnostic assay may be used to determine absence, presence, and excess expression of HSPP, and to monitor regulation of HSPP levels during therapeutic intervention.

[0183] In one aspect, hybridization with PCR probes which are capable of detecting polynucleotide sequences, including genomic sequences, encoding HSPP or closely related molecules may be used to identify nucleic acid sequences which encode HSPP. The specificity of the probe, whether it is made from a highly specific region, e.g., the 5' regulatory region, or from a less specific region, e.g., a conserved motif, and the stringency of the hybridization or amplification (maximal, high, intermediate, or low), will determine whether the probe identifies only naturally occurring sequences encoding HSPP, allelic variants, or related sequences.

[0184] Probes may also be used for the detection of related sequences, and should preferably have at least 50% sequence identity to any of the HSPP encoding sequences. The hybridization probes of the subject invention may be DNA or RNA and may be derived from the sequence of SEQ ID NO:135-268 or from genomic sequences including promoters, enhancers, and introns of the HSPP gene.

[0185] Means for producing specific hybridization probes for DNAs encoding HSPP include the cloning of polynucleotide sequences encoding HSPP or HSPP derivatives into vectors for the production of mRNA probes. Such vectors are known in the art, are commercially available, and may be used to synthesize RNA probes in vitro by means of the addition of the appropriate RNA polymerases and the appropriate labeled nucleotides. Hybridization probes may be labeled by a variety of reporter groups, for example, by radionuclides such as .sup.32P or .sup.35S, or by enzymatic labels, such as alkaline phosphatase coupled to the probe via avidin/biotin coupling systems, and the like.

[0186] Polynucleotide sequences encoding HSPP may be used for the diagnosis of disorders associated with expression of HSPP. Examples of such disorders include, but are not limited to, cell proliferative disorders such as actinic keratosis, arteriosclerosis, atherosclerosis, bursitis, cirrhosis, hepatitis, mixed connective tissue disease (MCTD), myelofibrosis, paroxysmal nocturnal hemoglobinuria, polycythemia vera, psoriasis, primary thrombocythemia, and cancers including adenocarcinoma, leukemia, lymphoma, melanoma, myeloma, sarcoma, teratocarcinoma, and, in particular, cancers of the adrenal gland, bladder, bone, bone marrow, brain, breast, cervix, gall bladder, ganglia, gastrointestinal tract, heart, kidney, liver, lung, muscle, ovary, pancreas, parathyroid, penis, prostate, salivary glands, skin, spleen, testis, thymus, thyroid, and uterus; inflammatory disorders, such as acquired immunodeficiency syndrome (AIDS), Addison's disease, adult respiratory distress syndrome, allergies, ankylosing spondylitis, amyloidosis, anemia, asthma, atherosclerosis, autoimmune hemolytic anemia, autoimmune thyroiditis, autoimmune polyenodocrinopathy-candidiasis-ectodermal dystrophy (APECED), bronchitis, cholecystitis, contact dermatitis, Crohn's disease, atopic dermatitis, dermatomyositis, diabetes mellitus, emphysema, episodic lymphopenia with lymphocytotoxins, erythroblastosis fetalis, erythema nodosum, atrophic gastritis, glomerulonephritis, Goodpasture's syndrome, gout, Graves' disease, Hashimoto's thyroiditis, hypereosinophilia, irritable bowel syndrome, multiple sclerosis, myasthenia gravis, myocardial or pericardial inflammation, osteoarthritis, osteoporosis, pancreatitis, polymyositis, psoriasis, Reiter's syndrome, rheumatoid arthritis, scleroderma, Sjogren's syndrome, systemic anaphylaxis, systemic lupus erythematosus, systemic sclerosis, thrombocytopenic purpura, ulcerative colitis, uveitis, Werner syndrome, complications of cancer, hemodialysis, and extracorporeal circulation, viral, bacterial, fungal, parasitic, protozoal, and helminthic infections, and trauma; cardiovascular disorders including disorders of the blood vessels such as arteriovenous fistula, atherosclerosis, hypertension, vasculitis, Raynaud's disease, aneurysms, arterial dissections, varicose veins, thrombophlebitis and phlebothrombosis, and vascular tumors; disorders of the heart such as congestive heart failure, ischemic heart disease, angina pectoris, myocardial infarction, hypertensive heart disease, degenerative valvular heart disease, calcific aortic valve stenosis, congenitally bicuspid aortic valve, mitral annular calcification, mitral valve prolapse, rheumatic fever and rheumatic heart disease, infective endocarditis, nonbacterial thrombotic endocarditis, endocarditis of systemic lupus erythematosus, carcinoid heart disease, cardiomyopathy, myocarditis, pericarditis, neoplastic heart disease, and congenital heart disease; and disorders of the lungs such as congenital lung anomalies, atelectasis, pulmonary congestion and edema, pulmonary embolism, pulmonary hemorrhage, pulmonary infarction, pulmonary hypertension, vascular sclerosis, obstructive pulmonary disease, restrictive pulmonary disease, chronic obstructive pulmonary disease, emphysema, chronic bronchitis, bronchial asthma, bronchiectasis, bacterial pneumonia, viral and mycoplasmal pneumonia, lung abscess, pulmonary tuberculosis, diffuse interstitial diseases, pneumoconioses, sarcoidosis, idiopathic pulmonary fibrosis, desquamative interstitial pneumonitis, hypersensitivity pneumonitis, pulmonary eosinophilia bronchiolitis obliterans-organizing pneumonia, diffuse pulmonary hemorrhage syndromes, Goodpasture's syndromes, idiopathic pulmonary hemosiderosis, pulmonary involvement in collagen-vascular disorders, pulmonary alveolar proteinosis, lung tumors, inflammatory and noninflammatory pleural effusions, pneumothorax, and pleural tumors; neurological disorders such as epilepsy, ischemic cerebrovascular disease, stroke, cerebral neoplasms, Alzheimer's disease, Pick's disease, Huntington's disease, dementia, Parkinson's disease and other extrapyramidal disorders, amyotrophic lateral sclerosis and other motor neuron disorders, progressive neural muscular atrophy, retinitis pigmentosa, hereditary ataxias, multiple sclerosis and other demyelinating diseases, bacterial and viral meningitis, brain abscess, subdural empyema, epidural abscess, suppurative intracranial thrombophlebitis, myelitis and radiculitis, viral central nervous system disease; prion diseases including kuru, Creutzfeldt-Jakob disease, and Gerstmann-Straussler-Scheinker syndrome; fatal familial insomnia, nutritional and metabolic diseases of the nervous system, neurofibromatosis, tuberous sclerosis, cerebelloretinal hemangioblastomatosis, encephalotrigeminal syndrome, mental retardation and other developmental disorders of the central nervous system, cerebral palsy, neuroskeletal disorders, autonomic nervous system disorders, cranial nerve disorders, spinal cord diseases, muscular dystrophy and other neuromuscular disorders, peripheral nervous system disorders, dermatomyositis and polymyositis; inherited, metabolic, endocrine, and toxic myopathies; myasthenia gravis, periodic paralysis; mental disorders including mood, anxiety, and schizophrenic disorders; akathesia, amnesia, catatonia, diabetic neuropathy, tardive dyskinesia, dystonias, paranoid psychoses, postherpetic neuralgia, and Tourette's disorder; reproductive disorders such as disorders of prolactin production; infertility, including tubal disease, ovulatory defects, and endometriosis; disruptions of the estrous cycle, disruptions of the menstrual cycle, polycystic ovary syndrome, ovarian hyperstimulation syndrome, endometrial and ovarian tumors, uterine fibroids, autoimmune disorders, ectopic pregnancies, and teratogenesis; cancer of the breast, fibrocystic breast disease, and galactorrhea; disruptions of spermatogenesis, abnormal sperm physiology, cancer of the testis, cancer of the prostate, benign prostatic hyperplasia, prostatitis, Peyronie's disease, carcinoma of the male breast, and gynecomastia; and developmental disorders, such as renal tubular acidosis, anemia, Cushing's syndrome, achondroplastic dwarfism, Duchenne and Becker muscular dystrophy, epilepsy, gonadal dysgenesis, WAGR syndrome (Wilms' tumor, aniridia, genitourinary abnormalities, and mental retardation), Smith-Magenis syndrome, myelodysplastic syndrome, hereditary mucoepithelial dysplasia, hereditary keratodermas, hereditary neuropathies such as Charcot-Marie-Tooth disease and neurofibromatosis, hypothyroidism, hydrocephalus, seizure disorders such as Syndenham's chorea and cerebral palsy, spina bifida, anencephaly, craniorachischisis, congenital glaucoma, cataract, and sensorineural hearing loss. The polynucleotide sequences encoding HSPP may be used in Southern or northern analysis, dot blot, or other membrane-based technologies; in PCR technologies; in dipstick, pin, and multiformat ELISA-like assays; and in microarrays utilizing fluids or tissues from patients to detect altered HSPP expression. Such qualitative or quantitative methods are well known in the art.

[0187] In a particular aspect, the nucleotide sequences encoding HSPP may be useful in assays that detect the presence of associated disorders, particularly those mentioned above. The nucleotide sequences encoding HSPP may be labeled by standard methods and added to a fluid or tissue sample from a patient under conditions suitable for the formation of hybridization complexes. After a suitable incubation period, the sample is washed and the signal is quantitated and compared with a standard value. If the amount of signal in the patient sample is significantly altered in comparison to a control sample then the presence of altered levels of nucleotide sequences encoding HSPP in the sample indicates the presence of the associated disorder. Such assays may also be used to evaluate the efficacy of a particular therapeutic treatment regimen in animal studies, in clinical trials, or to monitor the treatment of an individual patient.

[0188] In order to provide a basis for the diagnosis of a disorder associated with expression of HSPP, a normal or standard profile for expression is established. This may be accomplished by combining body fluids or cell extracts taken from normal subjects, either animal or human, with a sequence, or a fragment thereof, encoding HSPP, under conditions suitable for hybridization or amplification. Standard hybridization may be quantified by comparing the values obtained from normal subjects with values from an experiment in which a known amount of a substantially purified polynucleotide is used. Standard values obtained in this manner may be compared with values obtained from samples from patients who are symptomatic for a disorder. Deviation from standard values is used to establish the presence of a disorder.

[0189] Once the presence of a disorder is established and a treatment protocol is initiated, hybridization assays may be repeated on a regular basis to determine if the level of expression in the patient begins to approximate that which is observed in the normal subject. The results obtained from successive assays may be used to show the efficacy of treatment over a period ranging from several days to months.

[0190] With respect to cancer, the presence of an abnormal amount of transcript (either under- or overexpressed) in biopsied tissue from an individual may indicate a predisposition for the development of the disease, or may provide a means for detecting the disease prior to the appearance of actual clinical symptoms. A more definitive diagnosis of this type may allow health professionals to employ preventative measures or aggressive treatment earlier thereby preventing the development or further progression of the cancer.

[0191] Additional diagnostic uses for oligonucleotides designed from the sequences encoding HSPP may involve the use of PCR. These oligomers may be chemically synthesized, generated enzymatically, or produced in vitro. Oligomers will preferably contain a fragment of a polynucleotide encoding HSPP, or a fragment of a polynucleotide complementary to the polynucleotide encoding HSPP, and will be employed under optimized conditions for identification of a specific gene or condition. Oligomers may also be employed under less stringent conditions for detection or quantitation of closely related DNA or RNA sequences.

[0192] Methods which may also be used to quantitate the expression of HSPP include radiolabeling or biotinylating nucleotides, coamplification of a control nucleic acid, and interpolating results from standard curves. (See, e.g., Melby, P. C. et al. (1993) J. Immunol. Methods 159:235-244; Duplaa, C. et al. (1993) Anal. Biochem. 212:229-236.) The speed of quantitation of multiple samples may be accelerated by running the assay in an ELISA format where the oligomer of interest is presented in various dilutions and a spectrophotometric or colorimetric response gives rapid quantitation.

[0193] In further embodiments, oligonucleotides or longer fragments derived from any of the polynucleotide sequences described herein may be used as targets in a microarray. The microarray can be used to monitor the expression level of large numbers of genes simultaneously and to identify genetic variants, mutations, and polymorphisms. This information may be used to determine gene function, to understand the genetic basis of a disorder, to diagnose a disorder, and to develop and monitor the activities of therapeutic agents.

[0194] Microarrays may be prepared, used, and analyzed using methods known in the art. (See, e.g., Brennan, T. M. et al. (1995) U.S. Pat. No. 5,474,796; Schena, M. et al. (1996) Proc. Natl. Acad. Sci. 93:10614-10619; Baldeschweiler et al. (1995) PCT application WO95/251116; Shalon, D. et al. (1995) PCT application WO95/35505; Heller, R. A. et al. (1997) Proc. Natl. Acad. Sci. 94:2150-2155; and Heller, M. J. et al. (1997) U.S. Pat. No. 5,605,662.)

[0195] In another embodiment of the invention, nucleic acid sequences encoding HSPP may be used to generate hybridization probes useful in mapping the naturally occurring genomic sequence. The sequences may be mapped to a particular chromosome, to a specific region of a chromosome, or to artificial chromosome constructions, e.g., human artificial chromosomes (HACs), yeast artificial chromosomes (YACs), bacterial artificial chromosomes (BACs), bacterial P1 constructions, or single chromosome cDNA libraries. (See, e.g., Harrington, J. J. et al. (1997) Nat Genet. 15:345-355; Price, C. M. (1993) Blood Rev. 7:127-134; and Trask, B. J. (1991) Trends Genet. 7:149-154.)

[0196] Fluorescent in situ hybridization (FISH) may be correlated with other physical chromosome mapping techniques and genetic map data. (See, e.g., Heinz-Ulrich, et al. (1995) in Meyers, supra, pp. 965-968.) Examples of genetic map data can be found in various scientific journals or at the Online Mendelian Inheritance in Man (OMIM) site. Correlation between the location of the gene encoding HSPP on a physical chromosomal map and a specific disorder, or a predisposition to a specific disorder, may help define the region of DNA associated with that disorder. The nucleotide sequences of the invention may be used to detect differences in gene sequences among normal, carrier, and affected individuals.

[0197] In situ hybridization of chromosomal preparations and physical mapping techniques, such as linkage analysis using established chromosomal markers, may be used for extending genetic maps. Often the placement of a gene on the chromosome of another mammalian species, such as mouse, may reveal associated markers even if the number or arm of a particular human chromosome is not known. New sequences can be assigned to chromosomal arms by physical mapping. This provides valuable information to investigators searching for disease genes using positional cloning or other gene discovery techniques. Once the disease or syndrome has been crudely localized by genetic linkage to a particular genomic region, e.g., ataxia-telangiectasia to 11q22-23, any sequences mapping to that area may represent associated or regulatory genes for further investigation. (See, e.g., Gatti, R. A. et al. (1988) Nature 336:577-580.) The nucleotide sequence of the subject invention may also be used to detect differences in the chromosomal location due to translocation, inversion, etc., among normal, carrier, or affected individuals.

[0198] In another embodiment of the invention, HSPP, its catalytic or immunogenic fragments, or oligopeptides thereof can be used for screening libraries of compounds in any of a variety of drug screening techniques. The fragment employed in such screening may be free in solution, affixed to a solid support, borne on a cell surface, or located intracellularly. The formation of binding complexes between HSPP and the agent being tested may be measured.

[0199] Another technique for drug screening provides for high throughput screening of compounds having suitable binding affinity to the protein of interest. (See, e.g., Geysen, et al. (1984) PCT application WO84/03564.) In this method, large numbers of different small test compounds are synthesized on a solid substrate. The test compounds are reacted with HSPP, or fragments thereof, and washed. Bound HSPP is then detected by methods well known in the art. Purified HSPP can also be coated directly onto plates for use in the aforementioned drug screening techniques. Alternatively, non-neutralizing antibodies can be used to capture the peptide and immobilize it on a solid support.

[0200] In another embodiment, one may use competitive drug screening assays in which neutralizing antibodies capable of binding HSPP specifically compete with a test compound for binding HSPP. In this manner, antibodies can be used to detect the presence of any peptide which shares one or more antigenic determinants with HSPP.

[0201] In additional embodiments, the nucleotide sequences which encode HSPP may be used in any molecular biology techniques that have yet to be developed, provided the new techniques rely on properties of nucleotide sequences that are currently known, including, but not limited to, such properties as the triplet genetic code and specific base pair interactions.

[0202] Without further elaboration, it is believed that one skilled in the art can, using the preceding description, utilize the present invention to its fullest extent. The following preferred specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.

[0203] The disclosures of all applications, patents, and publications, mentioned above and below, in particular U.S. Ser. No. 60/090,762, U.S. Ser. No. 60/094,983, U.S. Ser. No. 60/102,686, and U.S. Ser. No. 60/112,129, are hereby expressly incorporated by reference.

Examples

[0204] I. Construction of cDNA Libraries

[0205] RNA was purchased from Clontech or isolated from tissues described in Table 4. Some tissues were homogenized and lysed in guanidinium isothiocyanate, while others were homogenized and lysed in phenol or in a suitable mixture of denaturants, such as TRIZOL (Life Technologies), a monophasic solution of phenol and guanidine isothiocyanate. The resulting lysates were centrifuged over CsCl cushions or extracted with chloroform. RNA was precipitated from the lysates with either isopropanol or sodium acetate and ethanol, or by other routine methods.

[0206] Phenol extraction and precipitation of RNA were repeated as necessary to increase RNA purity. In some cases, RNA was treated with DNase. For most libraries, poly(A+) RNA was isolated using oligo d(T)-coupled paramagnetic particles (Promega), OLIGOTEX latex particles (QIAGEN, Valencia Calif.), or an OLIGOTEX mRNA purification kit (QIAGEN). Alternatively, RNA was isolated directly from tissue lysates using other RNA isolation kits, e.g., the POLY(A)PURE mRNA purification kit (Ambion, Austin Tex.).

[0207] In some cases, Stratagene was provided with RNA and constructed the corresponding cDNA libraries. Otherwise, cDNA was synthesized and cDNA libraries were constructed with the UNIZAP vector system (Stratagene) or SUPERSCRIPT plasmid system (Life Technologies), using the recommended procedures or similar methods known in the art. (See, e.g., Ausubel, 1997, supra, units 5.1-6.6). Reverse transcription was initiated using oligo d(T) or random primers. Synthetic oligonucleotide adapters were ligated to double stranded cDNA, and the cDNA was digested with the appropriate restriction enzyme or enzymes. For most libraries, the cDNA was size-selected (300-1000 bp) using SEPHACRYL S1000, SEPHAROSE CL2B, or SEPHAROSE CL4B column chromatography (Amersham Pharmacia Biotech) or preparative agarose gel electrophoresis. cDNAs were ligated into compatible restriction enzyme sites of the polylinker of a suitable plasmid, e.g., PBLUESCRIPT plasmid (Stratagene), PSPORT1 plasmid (Life Technologies), or pINCY (Incyte Corporation, Palo Alto Calif.). Recombinant plasmids were transformed into competent E. coli cells including XL1-Blue, XL1-BlueMRF, or SOLR from Stratagene or DH5 , DH10B, or ElectroMAX DH10B from Life Technologies.

II. Isolation of cDNA Clones

[0208] Plasmids were recovered from host cells by in vivo excision, using the UNIZAP vector system (Stratagene) or cell lysis. Plasmids were purified using at least one of the following: a MAGIC or WIZARD minipreps DNA purification system (Promega); an AGTC miniprep purification kit (Edge Biosystems, Gaithersburg Md.); and QIAWELL 8 Plasmid, QIAWELL 8 Plus Plasmid, QIAWELL 8 Ultra Plasmid purification systems or the REAL Prep 96 plasmid kit from QIAGEN. Following precipitation, plasmids were resuspended in 0.1 ml of distilled water and stored, with or without lyophilization, at 4.degree. C.

[0209] Alternatively, plasmid DNA was amplified from host cell lysates using direct link PCR in a high-throughput format (Rao, V. B. (1994) Anal. Biochem. 216:1-14). Host cell lysis and thermal cycling steps were carried out in a single reaction mixture. Samples were processed and stored in 384-well plates, and the concentration of amplified plasmid DNA was quantified fluorometrically using PICOGREEN dye (Molecular Probes, Eugene Oreg.) and a Fluoroskan II fluorescence scanner (Labsystems Oy, Helsinki, Finland).

III. Sequencing and Analysis

[0210] The cDNAs were prepared for sequencing using the ABI CATALYST 800 (Perkin-Elmer) or the HYDRA microdispenser (Robbins Scientific) or MICROLAB 2200 (Hamilton) systems in combination with the PTC-200 thermal cyclers (MJ Research). The cDNAs were sequenced using the ABI PRISM 373 or 377 sequencing systems (Perkin-Elmer) and standard ABI protocols, base calling software, and kits. In one alternative, cDNAs were sequenced using the MEGABACE 1000 DNA sequencing system (Molecular Dynamics). In another alternative, the cDNAs were amplified and sequenced using the ABI PRISM BIGDYE terminator cycle sequencing ready reaction kit (Perkin-Elmer). In yet another alternative, cDNAs were sequenced using solutions and dyes from Amersham Pharmacia Biotech. Reading frames for the ESTs were determined using standard methods (reviewed in Ausubel, 1997, supra, unit 7.7). Some of the cDNA sequences were selected for extension using the techniques disclosed in Example V.

[0211] The polynucleotide sequences derived from cDNA, extension, and shotgun sequencing were assembled and analyzed using a combination of software programs which utilize algorithms well known to those skilled in the art. Table 5 summarizes the software programs, descriptions, references, and threshold parameters used. The first column of Table 5 shows the tools, programs, and algorithms used, the second column provides a brief description thereof, the third column presents the references which are incorporated by reference herein, and the fourth column presents, where applicable, the scores, probability values, and other parameters used to evaluate the strength of a match between two sequences (the higher the probability the greater the homology). Sequences were analyzed using MACDNASIS PRO software (Hitachi Software Engineering, South San Francisco Calif.) and LASERGENE software (DNASTAR).

[0212] The polynucleotide sequences were validated by removing vector, linker, and polyA sequences and by masking ambiguous bases, using algorithms and programs based on BLAST, dynamic programming, and dinucleotide nearest neighbor analysis. The sequences were then queried against a selection of public databases such as GenBank primate, rodent, mammalian, vertebrate, and eukaryote databases, and BLOCKS to acquire annotation, using programs based on BLAST, FASTA, and BLIMPS. The sequences were assembled into full length polynucleotide sequences using programs based on Phred, Phrap, and Consed, and were screened for open reading frames using programs based on GeneMark, BLAST, and FASTA. The full length polynucleotide sequences were translated to derive the corresponding full length amino acid sequences, and these full length sequences were subsequently analyzed by querying against databases such as the GenBank databases (described above), SwissProt, BLOCKS, PRINTS, Prosite, and Hidden Markov Model (HMM)-based protein family databases such as PFAM. HMM is a probalistic approach which analyzes consensus primary structures of gene families. (See, e.g., Eddy, S. R. (1996) Cur. Opin. Str. Biol. 6:361-365.)

[0213] The programs described above for the assembly and analysis of full length polynucleotide and amino acid sequences were also used to identify polynucleotide sequence fragments from SEQ ID NO:135-268. Fragments from about 20 to about 4000 nucleotides which are useful in hybridization and amplification technologies were described in The Invention section above.

IV. Northern Analysis

[0214] Northern analysis is a laboratory technique used to detect the presence of a transcript of a gene and involves the hybridization of a labeled nucleotide sequence to a membrane on which RNAs from a particular cell type or tissue have been bound. (See, e.g., Sambrook, supra, ch. 7; Ausubel, 1995, supra, ch. 4 and 16.)

[0215] Analogous computer techniques applying BLAST were used to search for identical or related molecules in nucleotide databases such as GenBank or LIFESEQ database (Incyte Corporation). This analysis is much faster than multiple membrane-based hybridizations. In addition, the sensitivity of the computer search can be modified to determine whether any particular match is categorized as exact or similar. The basis of the search is the product score, which is defined as:

% sequence identity.times.% maximum BLAST score/100

The product score takes into account both the degree of similarity between two sequences and the length of the sequence match. For example, with a product score of 40, the match will be exact within a 1% to 2% error, and, with a product score of 70, the match will be exact. Similar molecules are usually identified by selecting those which show product scores between 15 and 40, although lower scores may identify related molecules.

[0216] The results of northern analyses are reported as a percentage distribution of libraries in which the transcript encoding HSPP occurred. Analysis involved the categorization of cDNA libraries by organ/tissue and disease. The organ/tissue categories included cardiovascular, dermatologic, developmental, endocrine, gastrointestinal, hematopoietic/immune, musculoskeletal, nervous, reproductive, and urologic. The disease/condition categories included cancer, inflammation/trauma, cell proliferation, neurological, and pooled. For each category, the number of libraries expressing the sequence of interest was counted and divided by the total number of libraries across all categories. Percentage values of tissue-specific and disease- or condition-specific expression are reported in Table 3.

V. Extension of HSPP Encoding Polynucleotides

[0217] Full length nucleic acid sequences of SEQ ID NOs:135-229 were produced by extension of the component fragments described in Table 1, column 5, using oligonucleotide primers based on these fragments. For each nucleic acid sequence, one primer was synthesized to initiate extension of an antisense polynucleotide, and the other was synthesized to initiate extension of a sense polynucleotide. Primers were used to facilitate the extension of the known sequence "outward" generating amplicons containing new unknown nucleotide sequence for the region of interest. The initial primers were designed from the cDNA using OLIGO 4.06 (National Biosciences, Plymouth, Minn.), or another appropriate program, to be about 22 to 30 nucleotides in length, to have a GC content of about 50% or more, and to anneal to the target sequence at temperatures of about 68.degree. C. to about 72.degree. C. Any stretch of nucleotides which would result in hairpin structures and primer-primer dimerizations was avoided.

[0218] Selected human cDNA libraries (GIBCO BRL) were used to extend the sequence. If more than one extension is necessary or desired, additional sets of primers are designed to further extend the known region.

[0219] High fidelity amplification was obtained by following the instructions for the XL-PCR kit (The Perkin-Elmer Corp., Norwalk, Conn.) and thoroughly mixing the enzyme and reaction mix. PCR was performed using the PTC-200 thermal cycler (MJ Research, Inc., Watertown, Mass.), beginning with 40 pmol of each primer and the recommended concentrations of all other components of the kit, with the following parameters:

TABLE-US-00001 Step 1 94.degree. C. for 1 min (initial denaturation) Step 2 65.degree. C. for 1 min Step 3 68.degree. C. for 6 min Step 4 94.degree. C. for 15 sec Step 5 65.degree. C. for 1 min Step 6 68.degree. C. for 7 min Step 7 Repeat steps 4 through 6 for an additional 15 cycles Step 8 94.degree. C. for 15 sec Step 9 65.degree. C. for 1 min Step 10 68.degree. C. for 7:15 min Step 11 Repeat steps 8 through 10 for an additional 12 cycles Step 12 72.degree. C. for 8 min Step 13 4.degree. C. (and holding)

[0220] A 5 .mu.l to 10 .mu.l aliquot of the reaction mixture was analyzed by electrophoresis on a low concentration (about 0.6% to 0.8%) agarose mini-gel to determine which reactions were successful in extending the sequence. Bands thought to contain the largest products were excised from the gel, purified using QIAQUICK (QIAGEN Inc.), and trimmed of overhangs using Klenow enzyme to facilitate religation and cloning.

[0221] After ethanol precipitation, the products were redissolved in 13 .mu.l of ligation buffer, 1 .mu.l T4-DNA ligase (15 units) and 1 .mu.l T4 polynucleotide kinase were added, and the mixture was incubated at room temperature for 2 to 3 hours, or overnight at 16.degree. C. Competent E. coli cells (in 40 .mu.l of appropriate media) were transformed with 3 .mu.l of ligation mixture and cultured in 80 .mu.l of SOC medium. (See, e.g., Sambrook, supra, Appendix A, p. 2.) After incubation for one hour at 37.degree. C., the E. coli mixture was plated on Luria Bertani (LB) agar (See, e.g., Sambrook, supra, Appendix A, p. 1) containing carbenicillin (2.times.carb). The following day, several colonies were randomly picked from each plate and cultured in 150 .mu.l of liquid LB/2.times.carb medium placed in an individual well of an appropriate commercially-available sterile 96-well microtiter plate. The following day, 5 .mu.l of each overnight culture was transferred into a non-sterile 96-well plate and, after dilution 1:10 with water, 5 .mu.l from each sample was transferred into a PCR array.

[0222] For PCR amplification, 18 .mu.l of concentrated PCR reaction mix (3.3.times.) containing 4 units of rTth DNA polymerase, a vector primer, and one or both of the gene specific primers used for the extension reaction were added to each well. Amplification was performed using the following conditions:

TABLE-US-00002 Step 1 94.degree. C. for 60 sec Step 2 94.degree. C. for 20 sec Step 3 55.degree. C. for 30 sec Step 4 72.degree. C. for 90 sec Step 5 Repeat steps 2 through 4 for an additional 29 cycles Step 6 72.degree. C. for 180 sec Step 7 4.degree. C. (and holding)

[0223] Aliquots of the PCR reactions were run on agarose gels together with molecular weight markers. The sizes of the PCR products were compared to the original partial cDNAs, and appropriate clones were selected, ligated into plasmid, and sequenced.

[0224] The full length nucleic acid sequences of SEQ ID NO:230-268 were produced by extension of an appropriate fragment of the full length molecule using oligonucleotide primers designed from this fragment. One primer was synthesized to initiate 5' extension of the known fragment, and the other primer, to initiate 3' extension of the known fragment. The initial primers were designed using OLIGO 4.06 software (National Biosciences), or another appropriate program, to be about 22 to 30 nucleotides in length, to have a GC content of about 50% or more, and to anneal to the target sequence at temperatures of about 68.degree. C. to about 72.degree. C. Any stretch of nucleotides which would result in hairpin structures and primer-primer dimerizations was avoided.

[0225] Selected human cDNA libraries were used to extend the sequence. If more than one extension was necessary or desired, additional or nested sets of primers were designed.

[0226] High fidelity amplification was obtained by PCR using methods well known in the art. PCR was performed in 96-well plates using the PTC-200 thermal cycler (MJ Research, Inc.). The reaction mix contained DNA template, 200 nmol of each primer, reaction buffer containing Mg.sup.2+, (NH.sub.4).sub.2SO.sub.4, and y-mercaptoethanol, Taq DNA polymerase (Amersham Pharmacia Biotech), ELONGASE enzyme (Life Technologies), and Pfu DNA polymerase (Stratagene), with the following parameters for primer pair PCI A and PCI B: Step 1: 94.degree. C., 3 min; Step 2: 94.degree. C., 15 sec; Step 3: 60.degree. C., 1 min; Step 4: 68.degree. C., 2 min; Step 5: Steps 2, 3, and 4 repeated 20 times; Step 6: 68.degree. C., 5 min; Step 7: storage at 4.degree. C. In the alternative, the parameters for primer pair T7 and SK+ were as follows: Step 1: 94.degree. C., 3 min; Step 2: 94.degree. C., 15 sec; Step 3: 57.degree. C., 1 min; Step 4: 68.degree. C., 2 min; Step 5: Steps 2, 3, and 4 repeated 20 times; Step 6: 68.degree. C., 5 min; Step 7: storage at 4.degree. C.

[0227] The concentration of DNA in each well was determined by dispensing 100 l PICOGREEN quantitation reagent (0.25% (v/v) PICOGREEN; Molecular Probes, Eugene Oreg.) dissolved in 1.times.TE and 0.5 l of undiluted PCR product into each well of an opaque fluorimeter plate (Corning Costar, Acton Mass.), allowing the DNA to bind to the reagent. The plate was scanned in a Fluoroskan II (Labsystems Oy, Helsinki, Finland) to measure the fluorescence of the sample and to quantify the concentration of DNA. A 5 .mu.l to 10 .mu.l aliquot of the reaction mixture was analyzed by electrophoresis on a 1% agarose mini-gel to determine which reactions were successful in extending the sequence.

[0228] The extended nucleotides were desalted and concentrated, transferred to 384-well plates, digested with CviJI cholera virus endonuclease (Molecular Biology Research, Madison Wis.), and sonicated or sheared prior to religation into pUC 18 vector (Amersham Pharmacia Biotech). For shotgun sequencing, the digested nucleotides were separated on low concentration (0.6 to 0.8%) agarose gels, fragments were excised, and agar digested with Agar ACE (Promega). Extended clones were religated using T4 ligase (New England Biolabs, Beverly Mass.) into pUC 18 vector (Amersham Pharmacia Biotech), treated with Pfu DNA polymerase (Stratagene) to fill-in restriction site overhangs, and transfected into competent E. coli cells. Transformed cells were selected on antibiotic-containing media, individual colonies were picked and cultured overnight at 37.degree. C. in 384-well plates in LB/2.times.carb liquid media.

[0229] The cells were lysed, and DNA was amplified by PCR using Taq DNA polymerase (Amersham Pharmacia Biotech) and Pfu DNA polymerase (Stratagene) with the following parameters: Step 1: 94.degree. C., 3 min; Step 2: 94.degree. C., 15 sec; Step 3: 60.degree. C., 1 min; Step 4: 72.degree. C., 2 min; Step 5: steps 2, 3, and 4 repeated 29 times; Step 6: 72.degree. C., 5 min; Step 7: storage at 4.degree. C. DNA was quantified by PICOGREEN reagent (Molecular Probes) as described above. Samples with low DNA recoveries were reamplified using the same conditions as described above. Samples were diluted with 20% dimethysulphoxide (1:2, v/v), and sequenced using DYENAMIC energy transfer sequencing primers and the DYENAMIC DIRECT kit (Amersham Pharmacia Biotech) or the ABI PRISM BIGDYE Terminator cycle sequencing ready reaction kit (Perkin-Elmer).

[0230] In like manner, the nucleotide sequences of SEQ ID NO:135-268 are used to obtain 5' regulatory sequences using the procedure above, oligonucleotides designed for such extension, and an appropriate genomic library.

VI. Labeling and Use of Individual Hybridization Probes

[0231] Hybridization probes derived from SEQ ID NO:135-268 are employed to screen cDNAs, genomic DNAs, or mRNAs. Although the labeling of oligonucleotides, consisting of about 20 base pairs, is specifically described, essentially the same procedure is used with larger nucleotide fragments. Oligonucleotides are designed using state-of-the-art software such as OLIGO 4.06 software (National Biosciences) and labeled by combining 50 pmol of each oligomer, 250 .mu.Ci of [ -.sup.32P] adenosine triphosphate (Amersham Pharmacia Biotech), and T4 polynucleotide kinase (DuPont NEN, Boston Mass.). The labeled oligonucleotides are substantially purified using a SEPHADEX G-25 superfine size exclusion dextran bead column (Amersham Pharmacia Biotech). An aliquot containing 10.sup.7 counts per minute of the labeled probe is used in a typical membrane-based hybridization analysis of human genomic DNA digested with one of the following endonucleases: Ase I, Bgl II, Eco RI, Pst I, Xba1, or Pvu II (DuPont NEN).

[0232] The DNA from each digest is fractionated on a 0.7% agarose gel and transferred to nylon membranes (Nytran Plus, Schleicher & Schuell, Durham N.H.). Hybridization is carried out for 16 hours at 40.degree. C. To remove nonspecific signals, blots are sequentially washed at room temperature under increasingly stringent conditions up to 0.1.times. saline sodium citrate and 0.5% sodium dodecyl sulfate. After XOMAT-AR film (Eastman Kodak, Rochester N. Y.) is exposed to the blots to film for several hours, hybridization patterns are compared visually.

VII. Microarrays

[0233] A chemical coupling procedure and an ink jet device can be used to synthesize array elements on the surface of a substrate. (See, e.g., Baldeschweiler, supra.) An array analogous to a dot or slot blot may also be used to arrange and link elements to the surface of a substrate using thermal, UV, chemical, or mechanical bonding procedures. A typical array may be produced by hand or using available methods and machines and contain any appropriate number of elements. After hybridization, nonhybridized probes are removed and a scanner used to determine the levels and patterns of fluorescence. The degree of complementarity and the relative abundance of each probe which hybridizes to an element on the microarray may be assessed through analysis of the scanned images.

[0234] Full-length cDNAs, Expressed Sequence Tags (ESTs), or fragments thereof may comprise the elements of the microarray. Fragments suitable for hybridization can be selected using software well known in the art such as LASERGENE software (DNASTAR). Full-length cDNAs, ESTs, or fragments thereof corresponding to one of the nucleotide sequences of the present invention, or selected at random from a cDNA library relevant to the present invention, are arranged on an appropriate substrate, e.g., a glass slide. The cDNA is fixed to the slide using, e.g., UV cross-linking followed by thermal and chemical treatments and subsequent drying. (See, e.g., Schena, M. et al. (1995) Science 270:467-470; Shalon, D. et al. (1996) Genome Res. 6:639-645.) Fluorescent probes are prepared and used for hybridization to the elements on the substrate. The substrate is analyzed by procedures described above.

VIII. Complementary Polynucleotides

[0235] Sequences complementary to the HSPP-encoding sequences, or any parts thereof, are used to detect, decrease, or inhibit expression of naturally occurring HSPP. Although use of oligonucleotides comprising from about 15 to 30 base pairs is described, essentially the same procedure is used with smaller or with larger sequence fragments. Appropriate oligonucleotides are designed using OLIGO 4.06 software (National Biosciences) and the coding sequence of HSPP. To inhibit transcription, a complementary oligonucleotide is designed from the most unique 5' sequence and used to prevent promoter binding to the coding sequence. To inhibit translation, a complementary oligonucleotide is designed to prevent ribosomal binding to the HSPP-encoding transcript.

IX. Expression of HSPP

[0236] Expression and purification of HSPP is achieved using bacterial or virus-based expression systems. For expression of HSPP in bacteria, cDNA is subcloned into an appropriate vector containing an antibiotic resistance gene and an inducible promoter that directs high levels of cDNA transcription. Examples of such promoters include, but are not limited to, the trp-lac (tac) hybrid promoter and the T5 or T7 bacteriophage promoter in conjunction with the lac operator regulatory element. Recombinant vectors are transformed into suitable bacterial hosts, e.g., BL21(DE3). Antibiotic resistant bacteria express HSPP upon induction with isopropyl beta-D-thiogalactopyranoside (IPTG). Expression of HSPP in eukaryotic cells is achieved by infecting insect or mammalian cell lines with recombinant Autographica californica nuclear polyhedrosis virus (AcMNPV), commonly known as baculovirus. The nonessential polyhedrin gene of baculovirus is replaced with cDNA encoding HSPP by either homologous recombination or bacterial-mediated transposition involving transfer plasmid intermediates. Viral infectivity is maintained and the strong polyhedrin promoter drives high levels of cDNA transcription. Recombinant baculovirus is used to infect Spodoptera frugiperda (Sf9) insect cells in most cases, or human hepatocytes, in some cases. Infection of the latter requires additional genetic modifications to baculovirus. (See Engelhard, E. K. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3224-3227; Sandig, V. et al. (1996) Hum. Gene Ther. 7:1937-1945.)

[0237] In most expression systems, HSPP is synthesized as a fusion protein with, e.g., glutathione S-transferase (GST) or a peptide epitope tag, such as FLAG or 6-His, permitting rapid, single-step, affinity-based purification of recombinant fusion protein from crude cell lysates. GST, a 26-kilodalton enzyme from Schistosoma japonicum, enables the purification of fusion proteins on immobilized glutathione under conditions that maintain protein activity and antigenicity (Amersham Pharmacia Biotech). Following purification, the GST moiety can be proteolytically cleaved from HSPP at specifically engineered sites. FLAG, an 8-amino acid peptide, enables immunoaffinity purification using commercially available monoclonal and polyclonal anti-FLAG antibodies (Eastman Kodak). 6-His, a stretch of six consecutive histidine residues, enables purification on metal-chelate resins (QIAGEN). Methods for protein expression and purification are discussed in Ausubel (1995, supra, ch 10 and 16). Purified HSPP obtained by these methods can be used directly in the following activity assay.

X. Demonstration of HSPP Activity

[0238] HSPP-68

[0239] HSPP-68 activity is measured by determining the potassium current using voltage clamp analysis on single Xenopus laevis oocytes injected with HSPP-68 cRNA. HSPP-68 cRNA is synthesized in vitro from linearized HSPP-68 encoding plasmids using the T7 RNA polymerase and injected into oocytes.. Injected oocytes are used two to four days after injection. In a 0.3 ml perfusion chamber, a single oocyte is impaled with two standard microelectrodes (1-2.5 M ) filled with 3 M KCl. The oocyte is maintained under voltage clamp by using a Dagan TEV 200 amplifier, in buffer containing 96 mM NaCl, 2 mM KCl, 1.8 mM CaCl.sub.2, 2 mM MgCl.sub.2, 5 mM HEPES, pH 7.4 with NaOH. Stimulation of the preparation, data acquisition, and analysis is performed using a computer. All experiments are performed at room temperature (21-22.degree. C.). Following a depolarizing pulse, the characteristics of the resulting potassium current are measured via the recording electrode. The amount of potassium current that flows in response to a unit depolarization is proportional to the activity of HSPP-68 in the cell. (Duprat, F. et al. (1997) EMBO J. 16:5464-5471.)

HSPP-92

[0240] HSPP-92 protein phosphatase activity is measured by the hydrolysis of P-nitrophenyl phosphate (PNPP). HSPP-92 is incubated together with PNPP in HEPES buffer pH 7.5, in the presence of 0.1% b-mercaptoethanol at 37.degree. C. for 60 min. The reaction is stopped by the addition of 6 ml of 10 N NaOH and the increase in light absorbance at 410 nm resulting from the hydrolysis of PNPP is measured using a spectrophotometer. The increase in light absorbance is proportional to the activity of PP in the assay. (Diamond R. H. et al (1994) Mol Cell Biol 14:3752-62.)

[0241] Alternatively, HSPP, or biologically active fragments thereof, are labeled with .sup.125I Bolton-Hunter reagent. (See, e.g., Bolton et al. (1973) Biochem. J. 133:529.) Candidate molecules previously arrayed in the wells of a multi-well plate are incubated with the labeled HSPP, washed, and any wells with labeled HSPP complex are assayed. Data obtained using different concentrations of HSPP are used to calculate values for the number, affinity, and association of HSPP with the candidate molecules.

[0242] Alternatively, an assay for HSPP activity measures the expression of HSPP on the cell surface. cDNA encoding HSPP is subcloned into an appropriate mammalian expression vector suitable for high levels of cDNA expression. The resulting construct is transfected into a nonhuman cell line such as NIH3T3. Cell surface proteins are labeled with biotin using methods known in the art. Immunoprecipitations are performed using HSPP-specific antibodies, and immunoprecipitated samples are analyzed using SDS-PAGE and immunoblotting techniques. The ratio of labeled immunoprecipitant to unlabeled immunoprecipitant is proportional to the amount of HSPP expressed on the cell surface.

[0243] Alternatively, an assay for HSPP activity measures the amount of HSPP in secretory, membrane-bound organelles. Transfected cells as described above are harvested and lysed. The lysate is fractionated using methods known to those of skill in the art, for example, sucrose gradient ultracentrifugation. Such methods allow the isolation of subcellular components such as the Golgi apparatus, ER, small membrane-bound vesicles, and other secretory organelles. Immunoprecipitations from fractionated and total cell lysates are performed using HSPP-specific antibodies, and immunoprecipitated samples are analyzed using SDS-PAGE and immunoblotting techniques. The concentration of HSPP in secretory organelles relative to HSPP in total cell lysate is proportional to the amount of HSPP in transit through the secretory pathway.

XI. Functional Assays

[0244] HSPP function is assessed by expressing the sequences encoding HSPP at physiologically elevated levels in mammalian cell culture systems. cDNA is subcloned into a mammalian expression vector containing a strong promoter that drives high levels of cDNA expression. Vectors of choice include pCMV SPORT (Life Technologies) and pCR3.1 (Invitrogen, Carlsbad Calif.), both of which contain the cytomegalovirus promoter. 5-10 .mu.g of recombinant vector are transiently transfected into a human cell line, preferably of endothelial or hematopoietic origin, using either liposome formulations or electroporation. 1-2 .mu.g of an additional plasmid containing sequences encoding a marker protein are co-transfected. Expression of a marker protein provides a means to distinguish transfected cells from nontransfected cells and is a reliable predictor of cDNA expression from the recombinant vector. Marker proteins of choice include, e.g., Green Fluorescent Protein (GFP; Clontech), CD64, or a CD64-GFP fusion protein. Flow cytometry (FCM), an automated, laser optics-based technique, is used to identify transfected cells expressing GFP or CD64-GFP, and to evaluate properties, for example, their apoptotic state. FCM detects and quantifies the uptake of fluorescent molecules that diagnose events preceding or coincident with cell death. These events include changes in nuclear DNA content as measured by staining of DNA with propidium iodide; changes in cell size and granularity as measured by forward light scatter and 90 degree side light scatter; down-regulation of DNA synthesis as measured by decrease in bromodeoxyuridine uptake; alterations in expression of cell surface and intracellular proteins as measured by reactivity with specific antibodies; and alterations in plasma membrane composition as measured by the binding of fluorescein-conjugated Annexin V protein to the cell surface. Methods in flow cytometry are discussed in Ormerod, M. G. (1994) Flow Cytometry, Oxford, New York N.Y.

[0245] The influence of HSPP on gene expression can be assessed using highly purified populations of cells transfected with sequences encoding HSPP and either CD64 or CD64-GFP. CD64 and CD64-GFP are expressed on the surface of transfected cells and bind to conserved regions of human immunoglobulin G (IgG). Transfected cells are efficiently separated from nontransfected cells using magnetic beads coated with either human IgG or antibody against CD64 (DYNAL, Lake Success N. Y.). mRNA can be purified from the cells using methods well known by those of skill in the art. Expression of mRNA encoding HSPP and other genes of interest can be analyzed by northern analysis or microarray techniques.

XII. Production of HSPP Specific Antibodies

[0246] HSPP substantially purified using polyacrylamide gel electrophoresis (PAGE; see, e.g., Harrington, M. G. (1990) Methods Enzymol. 182:488-495), or other purification techniques, is used to immunize rabbits and to produce antibodies using standard protocols.

[0247] Alternatively, the HSPP amino acid sequence is analyzed using LASERGENE software (DNASTAR) to determine regions of high immunogenicity, and a corresponding oligopeptide is synthesized and used to raise antibodies by means known to those of skill in the art. Methods for selection of appropriate epitopes, such as those near the C-terminus or in hydrophilic regions are well described in the art. (See, e.g., Ausubel, 1995, supra, ch. 11.)

[0248] Typically, oligopeptides 15 residues in length are synthesized using an ABI 431A Peptide Synthesizer (Perkin-Elmer) using fmoc-chemistry and coupled to KLH (Sigma-Aldrich, St. Louis Mo.) by reaction with N-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) to increase immunogenicity. (See, e.g., Ausubel, 1995, supra.) Rabbits are immunized with the oligopeptide-KLH complex in complete Freund's adjuvant. Resulting antisera are tested for antipeptide activity by, for example, binding the peptide to plastic, blocking with 1% BSA, reacting with rabbit antisera, washing, and reacting with radio-iodinated goat anti-rabbit IgG.

XIII. Purification of Naturally Occurring HSPP Using Specific Antibodies

[0249] Naturally occurring or recombinant HSPP is substantially purified by immunoaffinity chromatography using antibodies specific for HSPP. An immunoaffinity column is constructed by covalently coupling anti-HSPP antibody to an activated chromatographic resin, such as CNBr-activated SEPHAROSE (Amersham Pharmacia Biotech). After the coupling, the resin is blocked and washed according to the manufacturer's instructions.

[0250] Media containing HSPP are passed over the immunoaffinity column, and the column is washed under conditions that allow the preferential absorbance of HSPP (e.g., high ionic strength buffers in the presence of detergent). The column is eluted under conditions that disrupt antibody/HSPP binding (e.g., a buffer of pH 2 to pH 3, or a high concentration of a chaotrope, such as urea or thiocyanate ion), and HSPP is collected.

XIV. Identification of Molecules which Interact with HSPP

[0251] HSPP, or biologically active fragments thereof, are labeled with .sup.125I Bolton-Hunter reagent. (See, e.g., Bolton et al. (1973) Biochem. J. 133:529.) Candidate molecules previously arrayed in the wells of a multi-well plate are incubated with the labeled HSPP, washed, and any wells with labeled HSPP complex are assayed. Data obtained using different concentrations of HSPP are used to calculate values for the number, affinity, and association of HSPP with the candidate molecules.

[0252] Various modifications and variations of the described methods and systems of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the invention which are obvious to those skilled in molecular biology or related fields are intended to be within the scope of the following claims.

Sequence CWU 1

1

269188PRTHomo sapiensmisc_featureIncyte Clone No 443531 1Met Ser Trp Trp Leu Cys Leu Pro Leu Gly Leu Phe Gly Ser Cys Leu1 5 10 15Ala Pro Ala Ala Ala Ala Ala Leu Ser Glu Phe Thr Gln Glu Gln His 20 25 30Asp Gly Ala Gln Pro Ser Pro Lys Cys Leu Ala Glu Glu Leu Gly Asp 35 40 45Ala Trp Thr Ile Gln Ile Glu Ala Asn Trp Lys Tyr Arg Ala Val Asn 50 55 60Thr Asn Gln Arg Gly Lys Leu Leu Ala Ser Glu Thr Trp Lys Gly Arg65 70 75 80Arg Asn Thr Phe Phe Phe Leu Pro 852128PRTHomo sapiensmisc_featureIncyte Clone No 632860 2Met Trp Pro Ala Gly Leu Gly Arg Ser Leu Leu Ala Gln Pro Ala Leu1 5 10 15Cys Ser Phe Met Gly Pro Gln Trp Ile Leu Gln Phe Cys Ser Trp Leu 20 25 30Glu Pro Arg Gln Leu Arg Trp Ser Trp Thr Glu Pro Pro Phe Thr Leu 35 40 45Leu Asp Ser Leu Gly Leu Arg Ala Ala Gln Asp Ser Cys Ser Phe Thr 50 55 60Thr Leu Val Pro Leu Thr Leu Asp Ser Ser Phe Met Thr Val Asn Val65 70 75 80Val Pro Phe Val Trp Thr Ser Ser Phe Phe Arg Ala Phe Gln Tyr Pro 85 90 95Val Thr Ser Pro Cys Arg Thr Lys Asn Thr Pro Leu Leu Ile Asp Gly 100 105 110Val Thr Arg Ile Gln Ala Thr Trp Pro Glu Ala Arg Ser Gln His Glu 115 120 1253111PRTHomo sapiensmisc_featureIncyte Clone No 670010 3Met Gly Leu Leu Leu Leu Val Leu Phe Leu Ser Leu Leu Pro Val Ala1 5 10 15Tyr Thr Ile Met Ser Leu Pro Pro Ser Phe Asp Cys Gly Pro Phe Arg 20 25 30Cys Arg Val Ser Val Ala Arg Glu His Leu Pro Ser Arg Gly Ser Leu 35 40 45Leu Arg Gly Pro Arg Pro Arg Ile Pro Val Leu Val Ser Cys Gln Pro 50 55 60Val Lys Gly His Gly Thr Leu Gly Glu Ser Pro Met Pro Phe Lys Arg65 70 75 80Val Phe Cys Gln Asp Gly Asn Val Arg Ser Phe Cys Val Cys Ala Val 85 90 95His Phe Ser Ser His Gln Pro Pro Val Ala Val Glu Cys Leu Lys 100 105 1104110PRTHomo sapiensmisc_featureIncyte Clone No 726498 4Met Trp Arg Leu Arg Arg Asn Leu Ala Leu Pro Pro Gly Lys Leu Ala1 5 10 15Trp Leu Tyr Leu Ser Val Phe Ser Gln Gly Ser Arg Ala Met Met Ser 20 25 30Leu Thr Glu Ile Arg Leu Lys His Met Leu Glu Ile Trp His Gly Arg 35 40 45Gln Ala Arg Ala Cys Glu Asn Leu Arg Asn Gln Thr Arg Val Ala Thr 50 55 60Lys Val Glu Pro Gln Lys Gly Arg Ser Thr Glu Ile Cys Cys Leu Ala65 70 75 80Val Val Pro Leu Asn Glu Val Val Gln Ser Ser Ile Leu Trp Trp Val 85 90 95Trp Ser Cys Cys Gln His Gln Glu Asp Lys Leu Gly Ala Lys 100 105 110578PRTHomo sapiensmisc_featureIncyte Clone No 795064 5Met Ala Glu Ser Gly Leu Thr Ser Leu Pro Gly Thr Ala Ser Trp Phe1 5 10 15Cys Phe Leu Pro Val Ser Gln Arg Lys Ala Thr Ser Lys Lys Leu Leu 20 25 30Leu Lys Ala Arg Lys Lys Ser Gly Phe Glu Leu Ser Val Thr Asp Ser 35 40 45Ser Glu Cys Phe Arg Val Thr Ala Ser Val Arg Gly Met Lys Asn Arg 50 55 60His Ala Lys Gly Asn Gly Cys Thr Arg Asp Pro Cys Phe Gly65 70 75688PRTHomo sapiensmisc_featureIncyte Clone No 924925 6Met Trp Pro Ser Gln Val Pro Leu Leu Ala Phe Cys Phe Leu Leu Val1 5 10 15Lys Ser Thr Ser Asn Ile Asn Leu Pro Thr Pro Pro Pro Ser Ser Leu 20 25 30Glu Asn Ser Ser Phe Val Val Ser Gln Arg Gly Asn Leu Ile Val Phe 35 40 45Gly Gly Gln Lys Lys Ala Thr Phe Arg Tyr His Phe Tyr Leu Asp Arg 50 55 60Met Pro Phe Tyr Ser Gln Ile Ser Val Tyr Phe Val Asn Gly Phe Arg65 70 75 80Val Asn Gly Tyr Leu Cys Asn Asn 857227PRTHomo sapiensmisc_featureIncyte Clone No 962390 7Met Gly Arg Pro Leu Leu Leu Pro Leu Leu Leu Leu Leu Gln Pro Pro1 5 10 15Ala Phe Leu Gln Pro Gly Gly Ser Thr Gly Ser Gly Pro Ser Tyr Leu 20 25 30Tyr Gly Val Thr Gln Pro Lys His Leu Ser Ala Ser Met Gly Gly Ser 35 40 45Val Glu Ile Pro Phe Ser Phe Tyr Tyr Pro Trp Glu Leu Ala Ile Val 50 55 60Pro Asn Val Arg Ile Ser Trp Arg Arg Gly His Phe His Gly Gln Ser65 70 75 80Phe Tyr Ser Thr Arg Pro Pro Ser Ile His Lys Asp Tyr Val Asn Arg 85 90 95Leu Phe Leu Asn Trp Thr Glu Gly Gln Glu Ser Gly Phe Leu Arg Ile 100 105 110Ser Asn Leu Arg Lys Glu Asp Gln Ser Val Tyr Phe Cys Arg Val Glu 115 120 125Leu Asp Thr Arg Arg Ser Gly Arg Gln Gln Leu Gln Ser Ile Lys Gly 130 135 140Thr Lys Leu Thr Ile Thr Gln Ala Val Thr Thr Thr Thr Thr Trp Arg145 150 155 160Pro Ser Ser Thr Thr Thr Ile Ala Gly Leu Arg Val Thr Glu Ser Lys 165 170 175Gly His Ser Glu Ser Trp His Leu Ser Leu Asp Thr Ala Ile Arg Val 180 185 190Ala Leu Ala Val Ala Val Leu Lys Thr Val Ile Leu Gly Leu Leu Cys 195 200 205Leu Leu Leu Leu Trp Trp Arg Arg Arg Lys Gly Ser Arg Ala Pro Ser 210 215 220Ser Asp Phe2258198PRTHomo sapiensmisc_featureIncyte Clone No 1259405 8Met Ala Thr Leu Trp Gly Gly Leu Leu Arg Leu Gly Ser Leu Leu Ser1 5 10 15Leu Ser Cys Leu Ala Leu Ser Val Leu Leu Leu Ala Gln Leu Ser Asp 20 25 30Ala Ala Lys Asn Phe Glu Asp Val Arg Cys Lys Cys Ile Cys Pro Pro 35 40 45Tyr Lys Glu Asn Ser Gly His Ile Tyr Asn Lys Asn Ile Ser Gln Lys 50 55 60Asp Cys Asp Cys Leu His Val Val Glu Pro Met Pro Val Arg Gly Pro65 70 75 80Asp Val Glu Ala Tyr Cys Leu Arg Cys Glu Cys Lys Tyr Glu Glu Arg 85 90 95Ser Ser Val Thr Ile Lys Val Thr Ile Ile Ile Tyr Leu Ser Ile Leu 100 105 110Gly Leu Leu Leu Leu Tyr Met Val Tyr Leu Thr Leu Val Glu Pro Ile 115 120 125Leu Lys Arg Arg Leu Phe Gly His Ala Gln Leu Ile Gln Ser Asp Asp 130 135 140Asp Ile Gly Asp His Gln Pro Phe Ala Asn Ala His Asp Val Leu Ala145 150 155 160Arg Ser Arg Ser Arg Ala Asn Val Leu Asn Lys Val Glu Tyr Ala Gln 165 170 175Gln Arg Trp Lys Leu Gln Val Gln Glu Gln Arg Lys Ser Val Phe Asp 180 185 190Arg His Val Val Leu Ser 195965PRTHomo sapiensmisc_featureIncyte Clone No 1297384 9Met Met Pro Arg Leu Leu Gly Leu Gly Gly Leu Phe Ser Phe Gly Gly1 5 10 15Leu Pro Leu Leu Leu Leu Phe Phe Gln Arg Ser Arg Ala Ser Leu Ala 20 25 30Ser Ser Ser Thr Gly Leu Trp Ile Asn Gln Leu Pro Lys Gly Cys Thr 35 40 45Cys Arg Val Val Trp Ala Cys Ile Pro Asp Val Leu Glu Tyr Ala Trp 50 55 60Met6510154PRTHomo sapiensmisc_featureIncyte Clone No 1299627 10Met Asp Ala Pro Arg Leu Pro Val Arg Pro Gly Val Leu Leu Pro Lys1 5 10 15Leu Val Leu Leu Phe Val Tyr Ala Asp Asp Cys Leu Ala Gln Cys Gly 20 25 30Lys Asp Cys Lys Ser Tyr Cys Cys Asp Gly Thr Thr Pro Tyr Cys Cys 35 40 45Ser Tyr Tyr Ala Tyr Ile Gly Asn Ile Leu Ser Gly Thr Ala Ile Ala 50 55 60Gly Ile Val Phe Gly Ile Val Phe Ile Met Gly Val Ile Ala Gly Ile65 70 75 80Ala Ile Cys Ile Cys Met Cys Met Lys Asn His Arg Ala Thr Arg Val 85 90 95Gly Ile Leu Arg Thr Thr His Ile Asn Thr Val Ser Ser Tyr Pro Gly 100 105 110Pro Pro Pro Tyr Gly His Asp His Glu Met Glu Tyr Cys Ala Asp Leu 115 120 125Pro Pro Pro Tyr Ser Pro Thr Pro Gln Gly Pro Ala Gln Arg Ser Pro 130 135 140Pro Pro Pro Tyr Pro Gly Asn Ala Arg Lys145 15011237PRTHomo sapiensmisc_featureIncyte Clone No 1306026 11Met Lys Pro Leu Val Leu Leu Val Ala Leu Leu Leu Trp Pro Ser Ser1 5 10 15Val Pro Ala Tyr Pro Ser Ile Thr Val Thr Pro Asp Glu Glu Gln Asn 20 25 30Leu Asn His Tyr Ile Gln Val Leu Glu Asn Leu Val Arg Ser Val Pro 35 40 45Ser Gly Glu Pro Gly Arg Glu Lys Lys Ser Asn Ser Pro Lys His Val 50 55 60Tyr Ser Ile Ala Ser Lys Gly Ser Lys Phe Lys Glu Leu Val Thr His65 70 75 80Gly Asp Ala Ser Thr Glu Asn Asp Val Leu Thr Asn Pro Ile Ser Glu 85 90 95Glu Thr Thr Thr Phe Pro Thr Gly Gly Phe Thr Pro Glu Ile Gly Lys 100 105 110Lys Lys His Thr Glu Ser Thr Pro Phe Trp Ser Ile Lys Pro Asn Asn 115 120 125Val Ser Ile Val Leu His Ala Glu Glu Pro Tyr Ile Glu Asn Glu Glu 130 135 140Pro Glu Pro Glu Pro Glu Pro Ala Ala Lys Gln Thr Glu Ala Pro Arg145 150 155 160Met Leu Pro Val Val Thr Glu Ser Ser Thr Ser Pro Tyr Val Thr Ser 165 170 175Tyr Lys Ser Pro Val Thr Thr Leu Asp Lys Ser Thr Gly Ile Glu Ile 180 185 190Ser Thr Glu Ser Glu Asp Val Pro Gln Leu Ser Gly Glu Thr Ala Ile 195 200 205Glu Lys Pro Glu Ser Trp Lys His Gln Arg Val Gly Tyr Asp Ala Phe 210 215 220Glu Lys Asn Leu Val Leu Ile Thr Met His Arg His Phe225 230 23512225PRTHomo sapiensmisc_featureIncyte Clone No 1316219 12Met Thr Pro Glu Gly Val Gly Leu Thr Thr Ala Leu Arg Val Leu Cys1 5 10 15Asn Val Ala Cys Pro Pro Pro Pro Val Glu Gly Gln Gln Lys Asp Leu 20 25 30Lys Trp Asn Leu Ala Val Ile Gln Leu Phe Ser Ala Glu Gly Met Asp 35 40 45Thr Phe Ile Arg Val Leu Gln Lys Leu Asn Ser Ile Leu Thr Gln Pro 50 55 60Trp Arg Leu His Val Asn Met Gly Thr Thr Leu His Arg Val Thr Thr65 70 75 80Ile Ser Met Ala Arg Cys Thr Leu Thr Leu Leu Lys Thr Met Leu Thr 85 90 95Glu Leu Leu Arg Gly Gly Ser Phe Glu Phe Lys Asp Met Arg Val Pro 100 105 110Ser Ala Leu Val Thr Leu His Met Leu Leu Cys Ser Ile Pro Leu Ser 115 120 125Gly Arg Leu Asp Ser Asp Glu Gln Lys Ile Gln Asn Asp Ile Ile Asp 130 135 140Ile Leu Leu Thr Phe Thr Gln Gly Val Asn Glu Lys Leu Thr Ile Ser145 150 155 160Glu Glu Thr Leu Ala Asn Asn Thr Trp Ser Leu Met Leu Lys Glu Val 165 170 175Leu Ser Ser Ile Leu Lys Val Pro Glu Gly Phe Phe Ser Gly Leu Ile 180 185 190Leu Leu Ser Glu Leu Leu Pro Leu Pro Leu Pro Met Gln Thr Thr Gln 195 200 205Val Ser Leu Pro Tyr Asn Met His Leu Ile Asn Asp Cys Ser Asn Thr 210 215 220Phe22513117PRTHomo sapiensmisc_featureIncyte Clone No 1329031 13Met Pro Ser Pro Gly Thr Val Cys Ser Leu Leu Leu Leu Gly Met Leu1 5 10 15Trp Leu Asp Leu Ala Met Ala Gly Ser Ser Phe Leu Ser Pro Glu His 20 25 30Gln Arg Val Gln Gln Arg Lys Glu Ser Lys Lys Pro Pro Ala Lys Leu 35 40 45Gln Pro Arg Ala Leu Ala Gly Trp Leu Arg Pro Glu Asp Gly Gly Gln 50 55 60Ala Glu Gly Ala Glu Asp Glu Leu Glu Val Arg Phe Asn Ala Pro Phe65 70 75 80Asp Val Gly Ile Lys Leu Ser Gly Val Gln Tyr Gln Gln His Ser Gln 85 90 95Ala Leu Gly Lys Phe Leu Gln Asp Ile Leu Trp Glu Glu Ala Lys Glu 100 105 110Ala Pro Ala Asp Lys 11514253PRTHomo sapiensmisc_featureIncyte Clone No 1483050 14Met Asp Asn Arg Phe Ala Thr Ala Phe Val Ile Ala Cys Val Leu Ser1 5 10 15Leu Ile Ser Thr Ile Tyr Met Ala Ala Ser Ile Gly Thr Asp Phe Trp 20 25 30Tyr Glu Tyr Arg Ser Pro Val Gln Glu Asn Ser Ser Asp Leu Asn Lys 35 40 45Ser Ile Trp Asp Glu Phe Ile Ser Asp Glu Ala Asp Glu Lys Thr Tyr 50 55 60Asn Asp Ala Leu Phe Arg Tyr Asn Gly Thr Val Gly Leu Trp Arg Arg65 70 75 80Cys Ile Thr Ile Pro Lys Asn Met His Trp Tyr Ser Pro Pro Glu Arg 85 90 95Thr Glu Ser Phe Asp Val Val Thr Lys Cys Val Ser Phe Thr Leu Thr 100 105 110Glu Gln Phe Met Glu Lys Phe Val Asp Pro Gly Asn His Asn Ser Gly 115 120 125Ile Asp Leu Leu Arg Thr Tyr Leu Trp Arg Cys Gln Phe Leu Leu Pro 130 135 140Phe Val Ser Leu Gly Leu Met Cys Phe Gly Ala Leu Ile Gly Leu Cys145 150 155 160Ala Cys Ile Cys Arg Ser Leu Tyr Pro Thr Ile Ala Thr Gly Ile Leu 165 170 175His Leu Leu Ala Gly Leu Cys Thr Leu Gly Ser Val Ser Cys Tyr Val 180 185 190Ala Gly Ile Glu Leu Leu His Gln Lys Leu Glu Leu Pro Asp Asn Val 195 200 205Ser Gly Glu Phe Gly Trp Ser Phe Cys Leu Ala Cys Val Ser Ala Pro 210 215 220Leu Gln Phe Met Ala Ser Ala Leu Phe Ile Trp Ala Ala His Thr Asn225 230 235 240Arg Lys Glu Tyr Thr Leu Met Lys Ala Tyr Arg Val Ala 245 25015171PRTHomo sapiensmisc_featureIncyte Clone No 1514160 15Met Ser Leu Pro Ile Pro Trp Leu Ser Leu Pro Pro Cys Pro Ile Leu1 5 10 15Gly Gln Pro Ala Gly Leu Leu Leu Trp Leu Phe Arg Pro Phe Ser Gln 20 25 30Cys Cys Gln Cys Pro Trp Glu Gly Arg Ala Ser Leu Arg His Pro Asn 35 40 45Gly Pro Ser Gly Cys Arg Glu Ala Glu Ala Trp Pro Gln Arg Ser Leu 50 55 60Leu Arg Gln Gln Leu Gln Gln Ala His Pro Leu Pro Thr Leu Pro Thr65 70 75 80Pro Glu Arg Leu Pro Glu Gln Met Leu Phe Pro Ser Ser Ser Ser Lys 85 90 95Pro Phe Ser Leu Leu Ser Leu Thr Ile Trp Ala Arg Leu Val Gly Arg 100 105 110Leu Thr Asn Arg Ile Cys Pro Val Pro Pro Gly Ser Val Ala Ser Ser 115 120 125Met Ser Leu Gln Ala Gly Arg Cys Gly Asn Pro Val Val Leu Pro Gln 130 135 140Pro Met Pro Pro Gly Leu Leu Cys Met Asn Glu Cys Ser Leu Val Pro145 150 155 160Gly Leu Gly Arg Gly Gln Val Asn Ser Arg Val 165 1701678PRTHomo sapiensmisc_featureIncyte Clone No 1603403 16Met Gly Ser Gly Leu Pro Leu Val Leu Leu Leu Thr Leu Leu Gly Ser1 5 10 15Ser His Gly Thr Gly Pro Gly Met Thr Leu Gln Leu Lys Leu Lys Glu 20 25 30Ser Phe Leu Thr Asn Ser Ser Tyr Glu Ser Ser Phe Leu Glu Leu Leu 35 40 45Glu Lys Leu Cys Leu Leu Leu His Leu Pro Ser Gly Thr Ser Val Thr 50 55 60Leu His His Ala Arg Ser Gln His His Val Val Cys Asn Thr65 70 751771PRTHomo sapiensmisc_featureIncyte Clone No 1652303 17Met Lys Leu Leu Ser Cys Leu Leu Phe Leu Lys Ala Pro Leu Tyr Pro1 5 10 15Thr Leu Cys Ser Lys Asp Pro Arg Ala Gly His Ser Leu Ile Cys Gly 20 25 30Gln Ala Gly Gln Ile Pro Glu Ala Gln Leu Gly Phe Ser Ser Asp Phe 35

40 45Lys Leu Cys Trp Cys Trp Asp Gln Gln Lys Ala Asn Val Gln Pro Thr 50 55 60His Arg Thr Val Arg Gly Leu65 7018188PRTHomo sapiensmisc_featureIncyte Clone No 1693358 18Met Val Pro Gly Ala Ala Gly Trp Cys Cys Leu Val Leu Trp Leu Pro1 5 10 15Ala Cys Val Ala Ala His Gly Phe Arg Ile His Asp Tyr Leu Tyr Phe 20 25 30Gln Val Leu Ser Pro Gly Asp Ile Arg Tyr Ile Phe Thr Ala Thr Pro 35 40 45Ala Lys Asp Phe Gly Gly Ile Phe His Thr Arg Tyr Glu Gln Ile His 50 55 60Leu Val Pro Ala Glu Pro Pro Glu Ala Cys Gly Glu Leu Ser Asn Gly65 70 75 80Phe Phe Ile Gln Asp Gln Ile Ala Leu Val Glu Arg Gly Gly Cys Ser 85 90 95Phe Leu Ser Lys Thr Arg Val Val Gln Glu His Gly Gly Arg Ala Val 100 105 110Ile Ile Ser Asp Asn Ala Val Asp Asn Asp Ser Phe Tyr Val Glu Met 115 120 125Ile Gln Asp Ser Thr Gln Arg Thr Ala Asp Ile Pro Ala Leu Phe Leu 130 135 140Leu Gly Arg Asp Gly Tyr Met Ile Arg Arg Ser Leu Glu Gln His Gly145 150 155 160Leu Pro Trp Ala Ile Ile Ser Ile Pro Val Asn Val Thr Ser Ile Pro 165 170 175Thr Phe Glu Leu Leu Gln Pro Pro Trp Thr Phe Trp 180 1851980PRTHomo sapiensmisc_featureIncyte Clone No 1707711 19Met Lys Ala Gln Pro Leu Glu Ala Leu Leu Leu Val Ala Leu Val Leu1 5 10 15Ser Phe Cys Gly Val Trp Phe Glu Asp Trp Leu Ser Lys Trp Arg Phe 20 25 30Gln Cys Ile Phe Gln Leu Ala His Gln Pro Ala Leu Val Asn Ile Gln 35 40 45Phe Arg Gly Thr Val Leu Gly Ser Glu Thr Phe Leu Gly Ala Glu Glu 50 55 60Asn Ser Ala Asp Val Arg Ser Trp Gln Thr Leu Ser Tyr Phe Glu Leu65 70 75 802080PRTHomo sapiensmisc_featureIncyte Clone No 1738735 20Met Ile Asp Leu Trp Leu Pro Ala Leu Phe Val Leu Val Ala Leu Glu1 5 10 15Ser Leu Leu Leu Ser Pro Cys Pro Gly Thr Ser Ser Thr Leu Thr Arg 20 25 30Thr Phe Phe Pro Ser Leu Val Ser Cys Val Gln Val Pro Phe Ser Trp 35 40 45Ile Pro Cys Leu Glu Cys Phe Leu Ile Tyr Phe Leu Ile Leu Ala Glu 50 55 60Asp Val Leu Gln Leu Phe Ser Gly Asn Ala Asn Met Gln Val Asn Gln65 70 75 802184PRTHomo sapiensmisc_featureIncyte Clone No 1749147 21Met Gln Arg Pro Phe Leu Ser Val Pro Cys Leu Leu Leu Leu Pro Ala1 5 10 15Arg Val Val Trp Gly Cys Trp Cys Phe Leu Pro Gly Glu Asp Gly Gly 20 25 30Gly Cys Pro Thr Pro Ser Ser Gly Arg Ile Lys Leu Leu Gln Gln Cys 35 40 45Leu Leu His Pro Ser Leu Arg Ser Ile Thr Val Ser Arg Arg Ser Ala 50 55 60Gln Leu Leu Cys Arg Leu Lys Leu Gln Asn His Ile Pro Lys Val Pro65 70 75 80Gly Lys Asn Val22171PRTHomo sapiensmisc_featureIncyte Clone No 1817722 22Met His Met Ile Leu Lys Val Leu Thr Thr Ala Leu Leu Leu Gln Ala1 5 10 15Ala Ser Ala Leu Ala Asn Tyr Ile His Phe Ser Ser Tyr Ser Lys Asp 20 25 30Gly Ile Gly Val Pro Phe Met Gly Ser Leu Ala Glu Phe Phe Asp Ile 35 40 45Ala Ser Gln Ile Gln Met Leu Tyr Leu Leu Leu Ser Leu Cys Met Gly 50 55 60Trp Thr Ile Val Arg Met Lys Lys Ser Gln Ser Arg Pro Leu Gln Trp65 70 75 80Asp Ser Thr Pro Ala Ser Thr Gly Ile Ala Val Phe Ile Val Met Thr 85 90 95Gln Ser Val Leu Leu Leu Trp Glu Gln Phe Glu Asp Ile Ser His His 100 105 110Ser Tyr His Ser His His Asn Leu Ala Gly Ile Leu Leu Ile Val Leu 115 120 125Arg Ile Cys Leu Ala Leu Ser Leu Gly Cys Gly Leu Tyr Gln Ile Ile 130 135 140Thr Val Glu Arg Ser Thr Leu Lys Arg Glu Phe Tyr Ile Thr Phe Ala145 150 155 160Lys Val Trp Val Trp Lys Glu Asn Gly Leu Phe 165 17023243PRTHomo sapiensmisc_featureIncyte Clone No 1831290 23Met Ser Ser Gly Thr Glu Leu Leu Trp Pro Gly Ala Ala Leu Leu Val1 5 10 15Leu Leu Gly Val Ala Ala Ser Leu Cys Val Arg Cys Ser Arg Pro Gly 20 25 30Ala Lys Arg Ser Glu Lys Ile Tyr Gln Gln Arg Ser Leu Arg Glu Asp 35 40 45Gln Gln Ser Phe Thr Gly Ser Arg Thr Tyr Ser Leu Val Gly Gln Ala 50 55 60Trp Pro Gly Pro Leu Ala Asp Met Ala Pro Thr Arg Lys Asp Lys Leu65 70 75 80Leu Gln Phe Tyr Pro Ser Leu Glu Asp Pro Ala Ser Ser Arg Tyr Gln 85 90 95Asn Phe Ser Lys Gly Ser Arg His Gly Ser Glu Glu Ala Tyr Ile Asp 100 105 110Pro Ile Ala Met Glu Tyr Tyr Asn Trp Gly Arg Phe Ser Lys Pro Pro 115 120 125Glu Asp Asp Asp Ala Asn Ser Tyr Glu Asn Val Leu Ile Cys Lys Gln 130 135 140Lys Thr Thr Glu Thr Gly Ala Gln Gln Glu Gly Ile Gly Gly Leu Cys145 150 155 160Arg Gly Asp Leu Ser Leu Ser Leu Ala Leu Lys Thr Gly Pro Thr Ser 165 170 175Gly Leu Cys Pro Ser Ala Ser Pro Glu Glu Asp Glu Glu Ser Glu Asp 180 185 190Tyr Gln Asn Ser Ala Ser Ile His Gln Trp Arg Glu Ser Arg Lys Val 195 200 205Met Gly Gln Leu Gln Arg Glu Ala Ser Pro Gly Pro Val Gly Ser Pro 210 215 220Asp Glu Glu Asp Gly Glu Pro Asp Tyr Val Asn Gly Glu Val Ala Ala225 230 235 240Thr Glu Ala24311PRTHomo sapiensmisc_featureIncyte Clone No 1831477 24Met Gly Val Pro Thr Ala Pro Glu Ala Gly Ser Trp Arg Trp Gly Ser1 5 10 15Leu Leu Phe Ala Leu Phe Leu Ala Ala Ser Leu Gly Pro Val Ala Ala 20 25 30Phe Lys Val Ala Thr Pro Tyr Ser Leu Tyr Val Cys Pro Glu Gly Gln 35 40 45Asn Val Thr Leu Thr Cys Arg Leu Leu Gly Pro Val Asp Lys Gly His 50 55 60Asp Val Thr Phe Tyr Lys Thr Trp Tyr Arg Ser Ser Arg Gly Glu Val65 70 75 80Gln Thr Cys Ser Glu Arg Arg Pro Ile Arg Asn Leu Thr Phe Gln Asp 85 90 95Leu His Leu His His Gly Gly His Gln Ala Ala Asn Thr Ser His Asp 100 105 110Leu Ala Gln Arg His Gly Leu Glu Ser Ala Ser Asp His His Gly Asn 115 120 125Phe Ser Ile Thr Met Arg Asn Leu Thr Leu Leu Asp Ser Gly Leu Tyr 130 135 140Cys Cys Leu Val Val Glu Ile Arg His His His Ser Glu His Arg Val145 150 155 160His Gly Ala Met Glu Leu Gln Val Gln Thr Gly Lys Asp Ala Pro Ser 165 170 175Asn Cys Val Val Tyr Pro Ser Ser Ser Gln Glu Ser Glu Asn Ile Thr 180 185 190Ala Ala Ala Leu Ala Thr Gly Ala Cys Ile Val Gly Ile Leu Cys Leu 195 200 205Pro Leu Ile Leu Leu Leu Val Tyr Lys Gln Arg Gln Ala Ala Ser Asn 210 215 220Arg Arg Ala Gln Glu Leu Val Arg Met Asp Ser Asn Ile Gln Gly Ile225 230 235 240Glu Asn Pro Gly Phe Glu Ala Ser Pro Pro Ala Gln Gly Ile Pro Glu 245 250 255Ala Lys Val Arg His Pro Leu Ser Tyr Val Ala Gln Arg Gln Pro Ser 260 265 270Glu Ser Gly Arg His Leu Leu Ser Glu Pro Ser Thr Pro Leu Ser Pro 275 280 285Pro Gly Pro Gly Asp Val Phe Phe Pro Ser Leu Asp Pro Val Pro Asp 290 295 300Ser Pro Asn Phe Glu Val Ile305 3102557PRTHomo sapiensmisc_featureIncyte Clone No 1841607 25Met Ala Ser Ser Cys Phe Ser Leu Ser Phe Pro Pro Leu Ser Leu Ala1 5 10 15Gly Ser Leu Ala Leu Trp Gly His Cys Cys Val Arg Leu Gly Cys Ser 20 25 30Phe Trp Ser Val Ser Ala Met Ala Gln Arg Leu Pro Ser Gln Asn Thr 35 40 45Tyr Asn Pro Pro Leu Cys Trp Ala Trp 50 552682PRTHomo sapiensmisc_featureIncyte Clone No 1852391 26Met Phe Ser Leu Phe Ser Cys Leu Leu Ala Cys Leu Leu Asp Leu Leu1 5 10 15Leu Ser Arg Val Ala Asp Glu Ala Phe Tyr Lys Gln Pro Phe Ala Asp 20 25 30Val Ile Gly Tyr Val Tyr Val Ala Lys Leu Ile Pro Phe Ser Thr Ser 35 40 45Asp Ser Phe Tyr Phe Cys Leu Glu Leu Met Leu Leu Leu Cys His Gln 50 55 60Leu Leu Cys Phe Leu Asn Tyr Phe Lys Leu Ala Leu Trp Gly Leu Pro65 70 75 80Lys Asn27115PRTHomo sapiensmisc_featureIncyte Clone No 1854555 27Met Ala Gly Thr Val Leu Gly Val Gly Ala Gly Val Phe Ile Leu Ala1 5 10 15Leu Leu Trp Val Ala Val Leu Leu Leu Cys Val Leu Leu Ser Arg Ala 20 25 30Ser Gly Ala Ala Arg Phe Ser Val Ile Phe Leu Phe Phe Gly Ala Val 35 40 45Ile Ile Thr Ser Val Leu Leu Leu Phe Pro Arg Ala Gly Glu Phe Pro 50 55 60Ala Pro Glu Val Glu Val Lys Ile Val Asp Asp Phe Phe Ile Gly Arg65 70 75 80Tyr Val Leu Leu Ala Phe Leu Ser Ala Ile Phe Leu Gly Gly Leu Phe 85 90 95Leu Val Leu Ile His Tyr Val Leu Glu Pro Ile Tyr Ala Lys Pro Leu 100 105 110His Ser Tyr 11528327PRTHomo sapiensmisc_featureIncyte Clone No 1855755 28Met Ala Glu Leu Pro Gly Pro Phe Leu Cys Gly Ala Leu Leu Gly Phe1 5 10 15Leu Cys Leu Ser Gly Leu Ala Val Glu Val Lys Val Pro Thr Glu Pro 20 25 30Leu Ser Thr Pro Leu Gly Lys Thr Ala Glu Leu Thr Cys Thr Tyr Ser 35 40 45Thr Ser Val Gly Asp Ser Phe Ala Leu Glu Trp Ser Phe Val Gln Pro 50 55 60Gly Lys Pro Ile Ser Glu Ser His Pro Ile Leu Tyr Phe Thr Asn Gly65 70 75 80His Leu Tyr Pro Thr Gly Ser Lys Ser Lys Arg Val Ser Leu Leu Gln 85 90 95Asn Pro Pro Thr Val Gly Val Ala Thr Leu Lys Leu Thr Asp Val His 100 105 110Pro Ser Asp Thr Gly Thr Tyr Leu Cys Gln Val Asn Asn Pro Pro Asp 115 120 125Phe Tyr Thr Asn Gly Leu Gly Leu Ile Asn Leu Thr Val Leu Val Pro 130 135 140Pro Ser Asn Pro Leu Cys Ser Gln Ser Gly Gln Thr Ser Val Gly Gly145 150 155 160Ser Thr Ala Leu Arg Cys Ser Ser Ser Glu Gly Ala Pro Lys Pro Val 165 170 175Tyr Asn Trp Val Arg Leu Gly Thr Phe Pro Thr Pro Ser Pro Gly Ser 180 185 190Met Val Gln Asp Glu Val Ser Gly Gln Leu Ile Leu Thr Asn Leu Ser 195 200 205Leu Thr Ser Ser Gly Thr Tyr Arg Cys Val Ala Thr Asn Gln Met Gly 210 215 220Ser Ala Ser Cys Glu Leu Thr Leu Ser Val Thr Glu Pro Ser Gln Gly225 230 235 240Arg Val Ala Gly Ala Leu Ile Gly Val Leu Leu Gly Val Leu Leu Leu 245 250 255Ser Val Ala Ala Phe Cys Leu Val Arg Phe Gln Lys Glu Arg Gly Lys 260 265 270Lys Pro Lys Glu Thr Tyr Gly Gly Ser Asp Leu Arg Glu Asp Ala Ile 275 280 285Ala Pro Gly Ile Ser Glu His Thr Cys Met Arg Ala Asp Ser Ser Lys 290 295 300Gly Phe Leu Glu Arg Pro Ser Ser Ala Ser Thr Val Thr Thr Thr Lys305 310 315 320Ser Lys Leu Pro Met Val Val 32529133PRTHomo sapiensmisc_featureIncyte Clone No 1861434 29Met Arg Met Ser Leu Ala Gln Arg Val Leu Leu Thr Trp Leu Phe Thr1 5 10 15Leu Leu Phe Leu Ile Met Leu Val Leu Lys Leu Asp Glu Lys Ala Pro 20 25 30Trp Asn Trp Phe Leu Ile Phe Ile Pro Val Trp Ile Phe Asp Thr Ile 35 40 45Leu Leu Val Leu Leu Ile Val Lys Met Ala Gly Arg Cys Lys Ser Gly 50 55 60Phe Asp Pro Arg His Gly Ser His Asn Ile Lys Lys Lys Ala Trp Tyr65 70 75 80Leu Ile Ala Met Leu Leu Lys Leu Ala Phe Cys Leu Ala Leu Cys Ala 85 90 95Lys Leu Glu Gln Phe Thr Thr Met Asn Leu Ser Tyr Val Phe Ile Pro 100 105 110Leu Trp Ala Leu Leu Ala Gly Ala Leu Thr Glu Leu Gly Tyr Asn Val 115 120 125Phe Phe Val Arg Asp 13030129PRTHomo sapiensmisc_featureIncyte Clone No 1872334 30Met Gly Leu Thr Leu Leu Leu Leu Leu Leu Leu Gly Leu Glu Gly Gln1 5 10 15Gly Ile Val Gly Ser Leu Pro Glu Val Leu Gln Ala Pro Val Gly Ser 20 25 30Ser Ile Leu Val Gln Cys His Tyr Arg Leu Gln Asp Val Lys Ala Gln 35 40 45Lys Val Trp Cys Arg Phe Leu Pro Glu Gly Cys Gln Pro Leu Val Ser 50 55 60Ser Ala Val Asp Arg Arg Ala Pro Ala Gly Arg Arg Thr Phe Leu Thr65 70 75 80Asp Leu Gly Gly Gly Leu Leu Gln Val Glu Met Val Thr Leu Gln Glu 85 90 95Glu Asp Ala Gly Glu Tyr Gly Cys Met Val Asp Gly Ala Arg Gly Pro 100 105 110Gln Ile Leu His Arg Val Ser Leu Asn Ile Leu Pro Pro Gly Glu Leu 115 120 125Ser31472PRTHomo sapiensmisc_featureIncyte Clone No 1877230 31Met Lys Phe Leu Ile Phe Ala Phe Phe Gly Gly Val His Leu Leu Ser1 5 10 15Leu Cys Ser Gly Lys Ala Ile Cys Lys Asn Gly Ile Ser Lys Arg Thr 20 25 30Phe Glu Glu Ile Lys Glu Glu Ile Ala Ser Cys Gly Asp Val Ala Lys 35 40 45Ala Ile Ile Asn Leu Ala Val Tyr Gly Lys Ala Gln Asn Arg Ser Tyr 50 55 60Glu Arg Leu Ala Leu Leu Val Asp Thr Val Gly Pro Arg Leu Ser Gly65 70 75 80Ser Lys Asn Leu Glu Lys Ala Ile Gln Ile Met Tyr Gln Asn Leu Gln 85 90 95Gln Asp Gly Leu Glu Lys Val His Leu Glu Pro Val Arg Ile Pro His 100 105 110Trp Glu Arg Gly Glu Glu Ser Ala Val Met Leu Glu Pro Arg Ile His 115 120 125Lys Ile Ala Ile Leu Gly Leu Gly Ser Ser Ile Gly Thr Pro Pro Glu 130 135 140Gly Ile Thr Ala Glu Val Leu Val Val Thr Ser Phe Asp Glu Leu Gln145 150 155 160Arg Arg Ala Ser Glu Ala Arg Gly Lys Ile Val Val Tyr Asn Gln Pro 165 170 175Tyr Ile Asn Tyr Ser Arg Thr Val Gln Tyr Arg Thr Gln Gly Ala Val 180 185 190Glu Ala Ala Lys Val Gly Ala Leu Ala Ser Leu Ile Arg Ser Val Ala 195 200 205Ser Phe Ser Ile Tyr Ser Pro His Thr Gly Ile Gln Glu Tyr Gln Asp 210 215 220Gly Val Pro Lys Ile Pro Thr Ala Cys Ile Thr Val Glu Asp Ala Glu225 230 235 240Met Met Ser Arg Met Ala Ser His Gly Ile Lys Ile Val Ile Gln Leu 245 250 255Lys Met Gly Ala Lys Thr Tyr Pro Asp Thr Asp Ser Phe Asn Thr Val 260 265 270Ala Glu Ile Thr Gly Ser Lys Tyr Pro Glu Gln Val Val Leu Val Ser 275 280 285Gly His Leu Asp Ser Trp Asp Val Gly Gln Gly Ala Met Asp Asp Gly 290 295 300Gly Gly Ala Phe Ile Ser Trp Glu Ala Leu Ser Leu Ile Lys Asp Leu305 310 315 320Gly Leu Arg Pro Lys Arg Thr Leu Arg Leu Val Leu Trp Thr Ala Glu 325 330 335Glu Gln Gly Gly Val Gly Ala Phe Gln Tyr Tyr Gln Leu His Lys Val 340

345 350Asn Ile Ser Asn Tyr Ser Leu Val Met Glu Ser Asp Ala Gly Thr Phe 355 360 365Leu Pro Thr Gly Leu Gln Phe Thr Gly Ser Glu Lys Ala Arg Ala Ile 370 375 380Met Glu Glu Val Met Ser Leu Leu Gln Pro Leu Asn Ile Thr Gln Val385 390 395 400Leu Ser His Gly Glu Gly Thr Asp Ile Asn Phe Trp Ile Gln Ala Gly 405 410 415Val Pro Gly Ala Ser Leu Leu Asp Asp Leu Tyr Lys Tyr Phe Phe Phe 420 425 430His His Ser His Gly Asp Thr Met Thr Val Met Asp Pro Lys Gln Met 435 440 445Asn Val Ala Ala Ala Val Trp Ala Val Val Ser Tyr Val Val Ala Asp 450 455 460Met Glu Glu Met Leu Pro Arg Ser465 4703293PRTHomo sapiensmisc_featureIncyte Clone No 1877885 32Met Ile His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala1 5 10 15Ala Ala Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr 20 25 30Pro Gly Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro 35 40 45Leu Leu Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile 50 55 60Val Gly Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln65 70 75 80Glu Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly 85 903392PRTHomo sapiensmisc_featureIncyte Clone No 1889269 33Met Asn Arg Pro Ser Ala Arg Asn Ala Leu Gly Asn Val Phe Val Ser1 5 10 15Glu Leu Leu Glu Thr Leu Ala Gln Leu Arg Glu Asp Arg Gln Val Arg 20 25 30Val Leu Leu Phe Arg Ser Gly Val Lys Gly Val Phe Cys Ala Gly Ala 35 40 45Asp Leu Lys Glu Arg Glu Gln Met Ser Glu Ala Glu Val Gly Val Phe 50 55 60Val Gln Arg Leu Arg Gly Leu Met Asn Asp Ile Gly Glu Asp Leu Gly65 70 75 80Val Gly Trp Arg Arg Gly Phe Gly Gly Pro Cys Arg 85 9034143PRTHomo sapiensmisc_featureIncyte Clone No 1890243 34Met Trp Ile Lys Gly Thr Met Lys Met Arg Gly Gly Lys Thr Ser Arg1 5 10 15Ser Ala Val Leu Pro Val Ala Gln Leu Thr Leu Ile Ala Ser Cys Phe 20 25 30Pro Asn Ser Gln Thr Val Leu Gly Thr Glu Gly Thr Leu Asp Val Glu 35 40 45Ser Ser Pro Leu Ala Leu Leu Thr Gly Leu Trp Ala Ser Pro Glu Ser 50 55 60Leu Ser Leu Tyr Leu Val Thr Leu Leu Cys Val Cys Pro Ala Leu Gln65 70 75 80Ser Cys Gln Gly Gln Gln Ala Asp Val Thr Leu Ala Pro Cys Glu Ile 85 90 95Phe Ile Pro Gln Thr Leu Ala Cys Glu Pro Phe Pro Ser Gln Trp Arg 100 105 110Ala Leu Lys Gly Ala Ser Leu Glu Ser Ser Ser Val Leu Trp Val Ala 115 120 125Pro Cys Arg Trp Pro Leu Thr Leu Arg Cys Ser Arg Val His Leu 130 135 1403589PRTHomo sapiensmisc_featureIncyte Clone No 1900433 35Met Glu Arg Val Thr Leu Ala Leu Leu Leu Leu Ala Gly Leu Thr Ala1 5 10 15Leu Glu Ala Asn Asp Pro Phe Ala Asn Lys Asp Asp Pro Phe Tyr Tyr 20 25 30Asp Trp Lys Asn Leu Gln Leu Ser Gly Leu Ile Cys Gly Gly Leu Leu 35 40 45Ala Ile Ala Gly Ile Ala Ala Val Leu Ser Gly Lys Cys Lys Tyr Lys 50 55 60Ser Ser Gln Lys Gln His Ser Pro Val Pro Glu Lys Ala Ile Pro Leu65 70 75 80Ile Thr Pro Gly Ser Ala Thr Thr Cys 8536560PRTHomo sapiensmisc_featureIncyte Clone No 1909441 36Met Ala Lys Lys Lys Leu Thr Glu Met Ile Pro Leu Cys Asn His Pro1 5 10 15Ala Ser Phe Val Lys Leu Phe Val Ala Leu Gly Pro Ile Ala Gly Pro 20 25 30Glu Glu Lys Lys Gln Leu Lys Ser Thr Met Leu Leu Met Ser Glu Asp 35 40 45Leu Thr Gly Glu Gln Ala Leu Ala Val Leu Gly Ala Met Gly Asp Met 50 55 60Glu Ser Arg Asn Ser Cys Leu Ile Lys Arg Val Thr Ser Val Leu His65 70 75 80Lys His Leu Asp Gly Tyr Lys Pro Leu Glu Leu Leu Lys Ile Thr Gln 85 90 95Glu Leu Thr Phe Leu His Phe Gln Arg Lys Glu Phe Phe Ala Lys Leu 100 105 110Arg Glu Leu Leu Leu Ser Tyr Leu Lys Asn Ser Phe Ile Pro Thr Glu 115 120 125Val Ser Val Leu Val Arg Ala Ile Ser Leu Leu Pro Ser Pro His Leu 130 135 140Asp Glu Val Gly Ile Ser Arg Ile Glu Ala Val Leu Pro Gln Cys Asp145 150 155 160Leu Asn Asn Leu Ser Ser Phe Ala Thr Ser Val Leu Arg Trp Ile Gln 165 170 175His Asp His Met Tyr Leu Asp Asn Met Thr Ala Lys Gln Leu Lys Leu 180 185 190Leu Gln Lys Leu Asp His Tyr Gly Arg Gln Arg Leu Gln His Ser Asn 195 200 205Ser Leu Asp Leu Leu Arg Lys Glu Leu Lys Ser Leu Lys Gly Asn Thr 210 215 220Phe Pro Glu Ser Leu Leu Glu Glu Met Ile Ala Thr Leu Gln His Phe225 230 235 240Met Asp Asp Ile Asn Tyr Ile Asn Val Gly Glu Ile Ala Ser Phe Ile 245 250 255Ser Ser Thr Asp Tyr Leu Ser Thr Leu Leu Leu Asp Arg Ile Ala Ser 260 265 270Val Ala Val Gln Gln Ile Glu Lys Ile His Pro Phe Thr Ile Pro Ala 275 280 285Ile Ile Arg Pro Phe Ser Val Leu Asn Tyr Asp Pro Pro Gln Arg Asp 290 295 300Glu Phe Leu Gly Thr Cys Val Gln His Leu Asn Ser Tyr Leu Gly Ile305 310 315 320Leu Asp Pro Phe Ile Leu Val Phe Leu Gly Phe Ser Leu Ala Thr Leu 325 330 335Glu Tyr Phe Pro Glu Asp Leu Leu Lys Ala Ile Phe Asn Ile Lys Phe 340 345 350Leu Ala Arg Leu Asp Ser Gln Leu Glu Ile Leu Ser Pro Ser Arg Ser 355 360 365Ala Arg Val Gln Phe His Leu Met Glu Leu Asn Arg Ser Val Cys Leu 370 375 380Glu Cys Pro Glu Phe Gln Ile Pro Trp Phe His Asp Arg Phe Cys Gln385 390 395 400Gln Tyr Asn Lys Gly Ile Gly Gly Met Asp Gly Thr Gln Gln Gln Ile 405 410 415Phe Lys Met Leu Ala Glu Val Leu Gly Gly Ile Asn Cys Val Lys Ala 420 425 430Ser Val Leu Thr Pro Tyr Tyr His Lys Val Asp Phe Glu Cys Ile Leu 435 440 445Asp Lys Arg Lys Lys Pro Leu Pro Tyr Gly Ser His Asn Ile Ala Leu 450 455 460Gly Gln Leu Pro Glu Met Pro Trp Glu Ser Asn Ile Glu Ile Val Gly465 470 475 480Ser Arg Leu Pro Pro Gly Ala Glu Arg Ile Ala Leu Glu Phe Leu Asp 485 490 495Ser Lys Ala Leu Cys Arg Asn Ile Pro His Met Lys Gly Lys Ser Ala 500 505 510Met Lys Lys Arg His Leu Glu Ile Leu Gly Tyr Arg Val Ile Gln Ile 515 520 525Ser Gln Phe Glu Trp Asn Ser Met Ala Leu Ser Thr Lys Asp Ala Arg 530 535 540Met Asp Tyr Leu Arg Glu Cys Ile Phe Gly Glu Val Lys Ser Cys Leu545 550 555 56037197PRTHomo sapiensmisc_featureIncyte Clone No 1932226 37Met Gly Val Pro Leu Gly Leu Gly Ala Ala Trp Leu Leu Ala Trp Pro1 5 10 15Gly Leu Ala Leu Pro Leu Val Ala Met Ala Ala Gly Gly Arg Trp Val 20 25 30Arg Gln Gln Gly Pro Arg Val Arg Arg Gly Ile Ser Arg Leu Trp Leu 35 40 45Arg Val Leu Leu Arg Leu Ser Pro Met Ala Phe Arg Ala Leu Gln Gly 50 55 60Cys Gly Ala Val Gly Asp Arg Gly Leu Phe Ala Leu Tyr Pro Lys Thr65 70 75 80Asn Lys Asp Gly Phe Arg Ser Arg Leu Pro Val Pro Gly Pro Arg Arg 85 90 95Arg Asn Pro Arg Thr Thr Gln His Pro Leu Ala Leu Leu Ala Arg Val 100 105 110Trp Val Leu Cys Lys Gly Trp Asn Trp Arg Leu Ala Arg Ala Ser Gln 115 120 125Gly Leu Ala Ser His Leu Pro Pro Trp Ala Ile His Thr Leu Ala Ser 130 135 140Trp Gly Leu Leu Arg Gly Glu Arg Pro Thr Arg Ile Pro Arg Leu Leu145 150 155 160Pro Arg Ser Gln Arg Gln Leu Gly Pro Pro Ala Ser Arg Gln Pro Leu 165 170 175Pro Gly Thr Leu Ala Gly Arg Arg Ser Arg Thr Arg Gln Ser Arg Ala 180 185 190Leu Pro Pro Trp Arg 19538437PRTHomo sapiensmisc_featureIncyte Clone No 1932647 38Met Ser Ala Val Leu Leu Leu Ala Leu Leu Gly Phe Ile Leu Pro Leu1 5 10 15Pro Gly Val Gln Ala Leu Leu Cys Gln Phe Gly Thr Val Gln His Val 20 25 30Trp Lys Val Ser Asp Leu Pro Arg Gln Trp Thr Pro Lys Asn Thr Ser 35 40 45Cys Asp Ser Gly Leu Gly Cys Gln Asp Thr Leu Met Leu Ile Glu Ser 50 55 60Gly Pro Gln Val Ser Leu Val Leu Ser Lys Gly Cys Thr Glu Ala Lys65 70 75 80Asp Gln Glu Pro Arg Val Thr Glu His Arg Met Gly Pro Gly Leu Ser 85 90 95Leu Ile Ser Tyr Thr Phe Val Cys Arg Gln Glu Asp Phe Cys Asn Asn 100 105 110Leu Val Asn Ser Leu Pro Leu Trp Ala Pro Gln Pro Pro Ala Asp Pro 115 120 125Gly Ser Leu Arg Cys Pro Val Cys Leu Ser Met Glu Gly Cys Leu Glu 130 135 140Gly Thr Thr Glu Glu Ile Cys Pro Lys Gly Thr Thr His Cys Tyr Asp145 150 155 160Gly Leu Leu Arg Leu Arg Gly Gly Gly Ile Phe Ser Asn Leu Arg Val 165 170 175Gln Gly Cys Met Pro Gln Pro Gly Cys Asn Leu Leu Asn Gly Thr Gln 180 185 190Glu Ile Gly Pro Val Gly Met Thr Glu Asn Cys Asn Arg Lys Asp Phe 195 200 205Leu Thr Cys His Arg Gly Thr Thr Ile Met Thr His Gly Asn Leu Ala 210 215 220Gln Glu Pro Thr Asp Trp Thr Thr Ser Asn Thr Glu Met Cys Glu Val225 230 235 240Gly Gln Val Cys Gln Glu Thr Leu Leu Leu Ile Asp Val Gly Leu Thr 245 250 255Ser Thr Leu Val Gly Thr Lys Gly Cys Ser Thr Val Gly Ala Gln Asn 260 265 270Ser Gln Lys Thr Thr Ile His Ser Ala Pro Pro Gly Val Leu Val Ala 275 280 285Ser Tyr Thr His Phe Cys Ser Ser Asp Leu Cys Asn Ser Ala Ser Ser 290 295 300Ser Ser Val Leu Leu Asn Ser Leu Pro Pro Gln Ala Ala Pro Val Pro305 310 315 320Gly Asp Arg Gln Cys Pro Thr Cys Val Gln Pro Leu Gly Thr Cys Ser 325 330 335Ser Gly Ser Pro Arg Met Thr Cys Pro Arg Gly Ala Thr His Cys Tyr 340 345 350Asp Gly Tyr Ile His Leu Ser Gly Gly Gly Leu Ser Thr Lys Met Ser 355 360 365Ile Gln Gly Cys Val Ala Gln Pro Ser Ser Phe Leu Leu Asn His Thr 370 375 380Arg Gln Ile Gly Ile Phe Ser Ala Arg Glu Lys Arg Asp Val Gln Pro385 390 395 400Pro Ala Ser Gln His Glu Gly Gly Gly Ala Glu Gly Leu Glu Ser Leu 405 410 415Thr Trp Gly Val Gly Leu Ala Leu Ala Pro Ala Leu Trp Trp Gly Val 420 425 430Val Cys Pro Ser Cys 43539330PRTHomo sapiensmisc_featureIncyte Clone No 2124245 39Met Glu Gly Ala Pro Pro Gly Ser Leu Ala Leu Arg Leu Leu Leu Phe1 5 10 15Val Ala Leu Pro Ala Ser Gly Trp Leu Thr Thr Gly Ala Pro Glu Pro 20 25 30Pro Pro Leu Ser Gly Ala Pro Gln Asp Gly Ile Arg Ile Asn Val Thr 35 40 45Thr Leu Lys Asp Asp Gly Asp Ile Ser Lys Gln Gln Val Val Leu Asn 50 55 60Ile Thr Tyr Glu Ser Gly Gln Val Tyr Val Asn Asp Leu Pro Val Asn65 70 75 80Ser Gly Val Thr Arg Ile Ser Cys Gln Thr Leu Ile Val Lys Asn Glu 85 90 95Asn Leu Glu Asn Leu Glu Glu Lys Glu Tyr Phe Gly Ile Val Ser Val 100 105 110Arg Ile Leu Val His Glu Trp Pro Met Thr Ser Gly Ser Ser Leu Gln 115 120 125Leu Ile Val Ile Gln Glu Glu Val Val Glu Ile Asp Gly Lys Gln Val 130 135 140Gln Gln Lys Asp Val Thr Glu Ile Asp Ile Leu Val Lys Asn Arg Gly145 150 155 160Val Leu Arg His Ser Asn Tyr Thr Leu Pro Leu Glu Glu Ser Met Leu 165 170 175Tyr Ser Ile Ser Arg Asp Ser Asp Ile Leu Phe Thr Leu Pro Asn Leu 180 185 190Ser Lys Lys Glu Ser Val Ser Ser Leu Gln Thr Thr Ser Gln Tyr Leu 195 200 205Ile Arg Asn Val Glu Thr Thr Val Asp Glu Asp Val Leu Pro Gly Lys 210 215 220Leu Pro Glu Thr Pro Leu Arg Ala Glu Pro Pro Ser Ser Tyr Lys Val225 230 235 240Met Cys Gln Trp Met Glu Lys Phe Arg Lys Asp Leu Cys Arg Phe Trp 245 250 255Ser Asn Val Phe Pro Val Phe Phe Gln Phe Leu Asn Ile Met Val Val 260 265 270Gly Ile Thr Gly Ala Ala Val Val Ile Thr Ile Leu Lys Val Phe Phe 275 280 285Pro Val Ser Glu Tyr Lys Gly Ile Leu Gln Leu Asp Lys Val Asp Val 290 295 300Ile Pro Val Thr Ala Ile Asn Leu Tyr Pro Asp Gly Pro Glu Lys Arg305 310 315 320Ala Glu Asn Leu Glu Asp Lys Thr Cys Ile 325 33040148PRTHomo sapiensmisc_featureIncyte Clone No 2132626 40Met Glu Thr Gly Ala Leu Arg Arg Pro Gln Leu Leu Pro Leu Leu Leu1 5 10 15Leu Leu Cys Gly Gly Cys Pro Arg Ala Gly Gly Cys Asn Glu Thr Gly 20 25 30Met Leu Glu Arg Leu Pro Leu Cys Gly Lys Ala Phe Ala Asp Met Met 35 40 45Gly Lys Val Asp Val Trp Lys Trp Cys Asn Leu Ser Glu Phe Ile Val 50 55 60Tyr Tyr Glu Ser Phe Thr Asn Cys Thr Glu Met Glu Ala Asn Val Val65 70 75 80Gly Cys Tyr Trp Pro Asn Pro Leu Ala Gln Gly Phe Ile Thr Gly Ile 85 90 95His Arg Gln Phe Phe Ser Asn Cys Thr Val Asp Arg Val His Leu Glu 100 105 110Asp Pro Pro Asp Glu Val Leu Ile Pro Leu Ile Val Ile Pro Val Val 115 120 125Leu Thr Val Ala Met Ala Gly Leu Val Val Trp Arg Ser Lys Arg Thr 130 135 140Asp Thr Leu Leu14541188PRTHomo sapiensmisc_featureIncyte Clone No 2280639 41Met Ala Pro Pro Pro Pro Ser Pro Gln Leu Leu Leu Leu Ala Ala Leu1 5 10 15Ala Arg Leu Leu Gly Pro Ser Glu Val Met Ala Gly Pro Ala Glu Glu 20 25 30Ala Gly Ala His Cys Pro Glu Ser Leu Trp Pro Leu Pro Pro Gln Val 35 40 45Ser Pro Arg Val Thr Tyr Thr Arg Val Ser Pro Gly Gln Ala Glu Asp 50 55 60Val Thr Phe Leu Tyr His Pro Cys Ala His Pro Trp Leu Lys Leu Gln65 70 75 80Leu Ala Leu Leu Ala Tyr Ala Cys Met Ala Asn Pro Ser Leu Thr Pro 85 90 95Asp Phe Ser Leu Thr Gln Asp Arg Pro Leu Val Leu Thr Ala Trp Gly 100 105 110Leu Ala Leu Glu Met Ala Trp Val Glu Pro Ala Trp Ala Ala His Trp 115 120 125Leu Met Arg Arg Arg Arg Arg Lys Gln Arg Lys Lys Lys Ala Trp Ile 130 135 140Tyr Cys Glu Ser Leu Ser Gly Pro Ala Pro Ser Glu Pro Thr Pro Gly145 150 155 160Arg Gly Arg Leu Cys Arg Arg Gly Cys Val Gln Ala Leu Ala Leu Ala 165 170 175Phe Ala Leu Arg Thr Gly Gly Pro Leu Ala Gln Arg 180 18542222PRTHomo sapiensmisc_featureIncyte Clone No 2292356 42Met Ala Ala Ala Ala Leu Thr

Ser Leu Ser Thr Ser Pro Leu Leu Leu1 5 10 15Gly Ala Pro Val Ala Ala Phe Ser Pro Glu Pro Gly Leu Glu Pro Trp 20 25 30Lys Glu Ala Leu Val Arg Pro Pro Gly Ser Tyr Ser Ser Ser Ser Asn 35 40 45Ser Gly Asp Trp Gly Trp Asp Leu Ala Ser Asp Gln Ser Ser Pro Ser 50 55 60Thr Pro Ser Pro Pro Leu Pro Pro Glu Ala Ala His Phe Leu Phe Gly65 70 75 80Glu Pro Thr Leu Arg Lys Arg Lys Ser Pro Ala Gln Val Met Phe Gln 85 90 95Cys Leu Trp Lys Ser Cys Gly Lys Val Leu Ser Thr Ala Ser Ala Met 100 105 110Gln Arg His Ile Arg Leu Val His Leu Gly Cys Gly Gly Ala Trp Gly 115 120 125Ala Ala Gly Pro Ala Gly Trp Leu Gly Leu Leu Gly Pro Ala Arg Pro 130 135 140Pro Leu Gln Leu Pro Leu Ala Gly Cys Val Ser Arg Arg Arg Gln Ala145 150 155 160Glu Pro Glu Gln Ser Asp Gly Glu Glu Asp Phe Tyr Tyr Thr Glu Leu 165 170 175Asp Val Gly Val Asp Thr Leu Thr Asp Gly Leu Ser Ser Leu Thr Pro 180 185 190Val Phe Pro Glu Gly Phe His Ala Ser Leu Pro Ser Pro Ala Leu Lys 195 200 205Leu Arg Arg Leu Gly Gly Thr Arg Gln Pro Arg Gln Tyr Pro 210 215 22043111PRTHomo sapiensmisc_featureIncyte Clone No 2349310 43Met Gly Pro Ser Ser Cys Leu Leu Leu Ile Leu Ile Pro Leu Leu Gln1 5 10 15Leu Ile Asn Leu Gly Ser Thr Gln Cys Ser Leu Asp Ser Val Met Asp 20 25 30Lys Lys Ile Lys Asp Val Leu Asn Ser Leu Glu Tyr Ser Pro Ser Pro 35 40 45Ile Ser Lys Lys Leu Ser Cys Ala Ser Val Lys Ser Gln Gly Arg Pro 50 55 60Ser Ser Cys Pro Ala Gly Met Ala Val Thr Gly Cys Ala Cys Gly Tyr65 70 75 80Gly Cys Gly Ser Trp Asp Val Gln Leu Glu Thr Thr Cys His Cys Gln 85 90 95Cys Ser Val Val Asp Trp Thr Thr Ala Arg Cys Cys His Leu Thr 100 105 11044341PRTHomo sapiensmisc_featureIncyte Clone No 2373227 44Met Val Pro Ala Ala Gly Ala Leu Leu Trp Val Leu Leu Leu Asn Leu1 5 10 15Gly Pro Arg Ala Ala Gly Ala Gln Gly Leu Thr Gln Thr Pro Thr Glu 20 25 30Met Gln Arg Val Ser Leu Arg Phe Gly Gly Pro Met Thr Arg Ser Tyr 35 40 45Arg Ser Thr Ala Arg Thr Gly Leu Pro Arg Lys Thr Arg Ile Ile Leu 50 55 60Glu Asp Glu Asn Asp Ala Met Ala Asp Ala Asp Arg Leu Ala Gly Pro65 70 75 80Ala Ala Ala Glu Leu Leu Ala Ala Thr Val Ser Thr Gly Phe Ser Arg 85 90 95Ser Ser Ala Ile Asn Glu Glu Asp Gly Ser Ser Glu Glu Gly Val Val 100 105 110Ile Asn Ala Gly Lys Asp Ser Thr Ser Arg Glu Leu Pro Ser Ala Thr 115 120 125Pro Asn Thr Ala Gly Ser Ser Ser Thr Arg Phe Ile Ala Asn Ser Gln 130 135 140Glu Pro Glu Ile Arg Leu Thr Ser Ser Leu Pro Arg Ser Pro Gly Arg145 150 155 160Ser Thr Glu Asp Leu Pro Gly Ser Gln Ala Thr Leu Ser Gln Trp Ser 165 170 175Thr Pro Gly Ser Thr Pro Ser Arg Trp Pro Ser Pro Ser Pro Thr Ala 180 185 190Met Pro Ser Pro Glu Asp Leu Arg Leu Val Leu Met Pro Trp Gly Pro 195 200 205Trp His Cys His Cys Lys Ser Gly Thr Met Ser Arg Ser Arg Ser Gly 210 215 220Lys Leu His Gly Leu Ser Gly Arg Leu Arg Val Gly Ala Leu Ser Gln225 230 235 240Leu Arg Thr Glu His Lys Pro Cys Thr Tyr Gln Gln Cys Pro Cys Asn 245 250 255Arg Leu Arg Glu Glu Cys Pro Leu Asp Thr Ser Leu Cys Thr Asp Thr 260 265 270Asn Cys Ala Ser Gln Ser Thr Thr Ser Thr Arg Thr Thr Thr Thr Pro 275 280 285Phe Pro Thr Ile His Leu Arg Ser Ser Pro Ser Leu Pro Pro Ala Ser 290 295 300Pro Cys Pro Ala Leu Ala Phe Trp Lys Arg Val Arg Ile Gly Leu Glu305 310 315 320Asp Ile Trp Asn Ser Leu Ser Ser Val Phe Thr Glu Met Gln Pro Ile 325 330 335Asp Arg Asn Gln Arg 34045148PRTHomo sapiensmisc_featureIncyte Clone No 2457682 45Met Ala Gly Leu Ala Ala Arg Leu Val Leu Leu Ala Gly Ala Ala Ala1 5 10 15Leu Ala Ser Gly Ser Gln Gly Asp Arg Glu Pro Val Tyr Arg Asp Cys 20 25 30Val Leu Gln Cys Glu Glu Gln Asn Cys Ser Gly Gly Ala Leu Asn His 35 40 45Phe Arg Ser Arg Gln Pro Ile Tyr Met Ser Leu Ala Gly Trp Thr Cys 50 55 60Arg Asp Asp Cys Lys Tyr Glu Cys Met Trp Val Thr Val Gly Leu Tyr65 70 75 80Leu Gln Glu Gly His Lys Val Pro Gln Phe His Gly Lys Trp Pro Phe 85 90 95Ser Arg Phe Leu Phe Phe Gln Glu Pro Ala Ser Ala Val Ala Ser Phe 100 105 110Leu Asn Gly Leu Ala Ser Leu Val Met Leu Cys Arg Tyr Arg Thr Phe 115 120 125Val Pro Ala Ser Ser Pro Met Tyr His Thr Cys Val Ala Phe Ala Trp 130 135 140Leu Ser Gly Arg1454687PRTHomo sapiensmisc_featureIncyte Clone No 2480426 46Met Arg Pro Leu Leu Val Leu Leu Leu Leu Gly Leu Ala Ala Gly Ser1 5 10 15Pro Pro Leu Asp Asp Asn Lys Ile Pro Ser Leu Cys Pro Gly Leu Pro 20 25 30Gly Pro Arg Gly Asp Pro Gly Pro Arg Gly Glu Ala Gly Pro Ala Gly 35 40 45Pro Thr Gly Leu Ala Gly Glu Cys Ser Val Pro Pro Arg Ser Ala Phe 50 55 60Ser Ala Lys Arg Ser Glu Ile Arg Val Pro Pro Leu Ser Asp Ala Pro65 70 75 80Leu Pro Ser Thr Ala Cys Trp 8547383PRTHomo sapiensmisc_featureIncyte Clone No 2503743 47Met Ala Gly Ile Pro Gly Leu Leu Phe Leu Leu Phe Phe Leu Leu Cys1 5 10 15Ala Val Gly Gln Val Ser Pro Tyr Ser Ala Pro Trp Lys Pro Thr Trp 20 25 30Pro Ala Tyr Arg Leu Pro Val Val Leu Pro Gln Ser Thr Leu Asn Leu 35 40 45Ala Lys Pro Asp Phe Gly Ala Glu Ala Lys Leu Glu Val Ser Ser Ser 50 55 60Cys Gly Pro Gln Cys His Lys Gly Thr Pro Leu Pro Thr Tyr Glu Glu65 70 75 80Ala Lys Gln Tyr Leu Ser Tyr Glu Thr Leu Tyr Ala Asn Gly Ser Arg 85 90 95Thr Glu Thr Gln Val Gly Ile Tyr Ile Leu Ser Ser Ser Gly Asp Gly 100 105 110Ala Gln His Arg Asp Ser Gly Ser Ser Gly Lys Ser Arg Arg Lys Arg 115 120 125Gln Ile Tyr Gly Tyr Asp Ser Arg Phe Ser Ile Phe Gly Lys Asp Phe 130 135 140Leu Leu Asn Tyr Pro Phe Ser Thr Ser Val Lys Leu Ser Thr Gly Cys145 150 155 160Thr Gly Thr Leu Val Ala Glu Lys His Val Leu Thr Ala Ala His Cys 165 170 175Ile His Asp Gly Lys Thr Tyr Val Lys Gly Thr Gln Lys Leu Arg Val 180 185 190Gly Phe Leu Lys Pro Lys Phe Lys Asp Gly Gly Arg Gly Ala Asn Asp 195 200 205Ser Thr Ser Ala Met Pro Glu Gln Met Lys Phe Gln Trp Ile Arg Val 210 215 220Lys Arg Thr His Val Pro Lys Gly Trp Ile Lys Gly Asn Ala Asn Asp225 230 235 240Ile Gly Met Asp Tyr Asp Tyr Ala Leu Leu Glu Leu Lys Lys Pro His 245 250 255Lys Arg Lys Phe Met Lys Ile Gly Val Ser Pro Pro Ala Lys Gln Leu 260 265 270Pro Gly Gly Arg Ile His Phe Ser Gly Tyr Asp Asn Asp Arg Pro Gly 275 280 285Asn Leu Val Tyr Arg Phe Cys Asp Val Lys Asp Glu Thr Tyr Asp Leu 290 295 300Leu Tyr Gln Gln Cys Asp Ala Gln Pro Gly Ala Ser Gly Ser Gly Val305 310 315 320Tyr Val Arg Met Trp Lys Arg Gln Gln Gln Lys Trp Glu Arg Lys Ile 325 330 335Ile Gly Ile Phe Ser Gly His Gln Trp Val Asp Met Asn Gly Ser Pro 340 345 350Gln Asp Phe Asn Val Ala Val Arg Ile Thr Pro Leu Lys Tyr Ala Gln 355 360 365Ile Cys Tyr Trp Ile Lys Gly Asn Tyr Leu Asp Cys Arg Glu Gly 370 375 38048109PRTHomo sapiensmisc_featureIncyte Clone No 2537684 48Met Leu Leu Pro Ala Leu Cys Ala Trp Leu Leu Trp Val Pro Trp Cys1 5 10 15Leu Leu Val Ala Gly Ser Gly Arg Ser Gly Gly Glu Leu Cys Cys Ser 20 25 30Ser Tyr Gly Val Ser Val Ile Ser Val Trp Ser Lys Cys Ser Val Cys 35 40 45Arg Cys Leu Met Gly Ser Val Pro Arg Ile Phe Phe Ala Phe Tyr Pro 50 55 60Ile Ala Trp Leu Pro Leu Pro Gly Ser Gln Gly Cys Trp Ser Arg Ser65 70 75 80Trp Glu Trp Pro Leu Val Glu Pro Ala Ser Cys Leu Val Cys Leu Cys 85 90 95Phe Thr Phe Gly Val Leu Ser Gly Val Val Ala Val Lys 100 10549185PRTHomo sapiensmisc_featureIncyte Clone No 2593853 49Met Lys Phe Thr Ile Val Phe Ala Gly Leu Leu Gly Val Phe Leu Ala1 5 10 15Pro Ala Leu Ala Asn Tyr Asn Ile Asn Val Asn Asp Asp Asn Asn Asn 20 25 30Ala Gly Ser Gly Gln Gln Ser Val Ser Val Asn Asn Glu His Asn Val 35 40 45Ala Asn Val Asp Asn Asn Asn Gly Trp Asp Ser Trp Asn Ser Ile Trp 50 55 60Asp Tyr Gly Asn Gly Phe Ala Ala Thr Arg Leu Phe Gln Lys Lys Thr65 70 75 80Cys Ile Val His Lys Met Asn Lys Glu Val Met Pro Ser Ile Gln Ser 85 90 95Leu Asp Ala Leu Val Lys Glu Lys Lys Leu Gln Gly Lys Gly Pro Gly 100 105 110Gly Pro Pro Pro Lys Gly Leu Met Tyr Ser Val Asn Pro Asn Lys Val 115 120 125Asp Asp Leu Ser Lys Phe Gly Lys Asn Ile Ala Asn Met Cys Arg Gly 130 135 140Ile Pro Thr Tyr Met Ala Glu Glu Met Gln Glu Ala Ser Leu Phe Phe145 150 155 160Tyr Ser Gly Thr Cys Tyr Thr Thr Ser Val Leu Trp Ile Val Asp Ile 165 170 175Ser Phe Cys Gly Asp Thr Val Glu Asn 180 18550110PRTHomo sapiensmisc_featureIncyte Clone No 2622354 50Met Ala Pro Arg Gly Cys Ile Val Ala Val Phe Ala Ile Phe Cys Ile1 5 10 15Ser Arg Leu Leu Cys Ser His Gly Ala Pro Val Ala Pro Met Thr Pro 20 25 30Tyr Leu Met Leu Cys Gln Pro His Lys Arg Cys Gly Asp Lys Phe Tyr 35 40 45Asp Pro Leu Gln His Cys Cys Tyr Asp Asp Ala Val Val Pro Leu Ala 50 55 60Arg Thr Gln Thr Cys Gly Asn Cys Thr Phe Arg Val Cys Phe Glu Gln65 70 75 80Cys Cys Pro Trp Thr Phe Met Val Lys Leu Ile Asn Gln Asn Cys Asp 85 90 95Ser Ala Arg Thr Ser Asp Asp Arg Leu Cys Arg Ser Val Ser 100 105 11051126PRTHomo sapiensmisc_featureIncyte Clone No 2641377 51Met Trp Leu Gly Ser Trp Leu Thr Ser Leu Leu Leu Ser Pro Tyr Gly1 5 10 15Ser Gly Trp Glu Lys Val Pro Cys Cys Val Thr Gly His Leu Arg Ser 20 25 30Cys Ser Cys Cys Leu Leu Gly Leu Ala Gly Val Gln Ser Asp His Phe 35 40 45Ser Glu Gly Phe Phe Ser Glu Tyr Ser Ser Asp Val Leu Pro Trp Gly 50 55 60Arg Arg Ser Phe Leu Pro Gln Gly Asp Ala Ser Leu Leu Ala Cys Glu65 70 75 80Cys Phe Leu His Leu Gln Val Val Trp Gly Gln Phe Cys Leu Leu Glu 85 90 95Ala Trp Ala Gly Phe Thr Glu Gly Ser Met Pro Ala Pro Ser Cys Arg 100 105 110Val His Phe Trp Cys Arg Val Asn Thr Cys Ala Phe Met Ser 115 120 12552488PRTHomo sapiensmisc_featureIncyte Clone No 2674857 52Met Ala Gly Lys Gly Ser Ser Gly Arg Arg Pro Leu Leu Leu Gly Leu1 5 10 15Leu Val Ala Val Ala Thr Val His Leu Val Ile Cys Pro Tyr Thr Lys 20 25 30Val Glu Glu Ser Phe Asn Leu Gln Ala Thr His Asp Leu Leu Tyr His 35 40 45Trp Gln Asp Leu Glu Gln Tyr Asp His Leu Glu Phe Pro Gly Val Val 50 55 60Pro Arg Thr Phe Leu Gly Pro Val Val Ile Ala Val Phe Ser Ser Pro65 70 75 80Ala Val Tyr Val Leu Ser Leu Leu Glu Met Ser Lys Phe Tyr Ser Gln 85 90 95Leu Ile Val Arg Gly Val Leu Gly Leu Gly Val Ile Phe Gly Leu Trp 100 105 110Thr Leu Gln Lys Glu Val Arg Arg His Phe Gly Ala Met Val Ala Thr 115 120 125Met Phe Cys Trp Val Thr Ala Met Gln Phe His Leu Met Phe Tyr Cys 130 135 140Thr Arg Thr Leu Pro Asn Val Leu Ala Leu Pro Val Val Leu Leu Ala145 150 155 160Leu Ala Ala Trp Leu Arg His Glu Trp Ala Arg Phe Ile Trp Leu Ser 165 170 175Ala Phe Ala Ile Ile Val Phe Arg Val Glu Leu Cys Leu Phe Leu Gly 180 185 190Leu Leu Leu Leu Leu Ala Leu Gly Asn Arg Lys Val Ser Val Val Arg 195 200 205Ala Leu Arg His Ala Val Pro Ala Gly Ile Leu Cys Leu Gly Leu Thr 210 215 220Val Ala Val Asp Ser Tyr Phe Trp Arg Gln Leu Thr Trp Pro Glu Gly225 230 235 240Lys Val Leu Trp Tyr Asn Thr Val Leu Asn Lys Ser Ser Asn Trp Gly 245 250 255Thr Ser Pro Leu Leu Trp Tyr Phe Tyr Ser Ala Leu Pro Arg Gly Leu 260 265 270Gly Cys Ser Leu Leu Phe Ile Pro Leu Gly Leu Val Asp Arg Arg Thr 275 280 285His Ala Pro Thr Val Leu Ala Leu Gly Phe Met Ala Leu Tyr Ser Leu 290 295 300Leu Pro His Lys Glu Leu Arg Phe Ile Ile Tyr Ala Phe Pro Met Leu305 310 315 320Asn Ile Thr Ala Ala Arg Gly Cys Ser Tyr Leu Leu Asn Asn Tyr Lys 325 330 335Lys Ser Trp Leu Tyr Lys Ala Gly Ser Leu Leu Val Ile Gly His Leu 340 345 350Val Val Asn Ala Ala Tyr Ser Ala Thr Ala Leu Tyr Val Ser His Phe 355 360 365Asn Tyr Pro Gly Gly Val Ala Met Gln Arg Leu His Gln Leu Val Pro 370 375 380Pro Gln Thr Asp Val Leu Leu His Ile Asp Val Ala Ala Ala Gln Thr385 390 395 400Gly Val Ser Arg Phe Leu Gln Val Asn Ser Ala Trp Arg Tyr Asp Lys 405 410 415Arg Glu Asp Val Gln Pro Gly Thr Gly Met Leu Ala Tyr Thr His Ile 420 425 430Leu Met Glu Ala Ala Pro Gly Leu Leu Ala Leu Tyr Arg Asp Thr His 435 440 445Arg Val Leu Ala Ser Val Val Gly Thr Thr Gly Val Ser Leu Asn Leu 450 455 460Thr Gln Leu Pro Pro Phe Asn Val His Leu Gln Thr Lys Leu Val Leu465 470 475 480Leu Glu Arg Leu Pro Arg Pro Ser 48553197PRTHomo sapiensmisc_featureIncyte Clone No 2758485 53Met Ser Pro Arg Arg Thr Leu Pro Arg Pro Leu Ser Leu Cys Leu Ser1 5 10 15Leu Cys Leu Cys Leu Cys Leu Ala Ala Ala Leu Gly Ser Ala Gln Ser 20 25 30Gly Ser Cys Arg Asp Lys Lys Asn Cys Lys Val Val Phe Ser Gln Gln 35 40 45Glu Leu Arg Lys Arg Leu Thr Pro Leu Gln Tyr His Val Thr Gln Glu 50 55 60Lys Gly Thr Glu Ser Ala Phe Glu Gly Glu Tyr Thr His His Lys Asp65 70 75 80Pro Gly Ile Tyr Lys Cys Val Val Cys Gly Thr Pro Leu Phe Lys Ser 85

90 95Glu Thr Lys Phe Asp Ser Gly Ser Gly Trp Pro Ser Phe His Asp Val 100 105 110Ile Asn Ser Glu Ala Ile Thr Phe Thr Asp Asp Phe Ser Tyr Gly Met 115 120 125His Arg Val Glu Thr Ser Cys Ser Gln Cys Gly Ala His Leu Gly His 130 135 140Ile Phe Asp Asp Gly Pro Arg Pro Thr Gly Lys Arg Tyr Cys Ile Asn145 150 155 160Ser Ala Ala Leu Ser Phe Thr Pro Ala Asp Ser Ser Gly Thr Ala Glu 165 170 175Gly Gly Ser Gly Val Ala Ser Pro Ala Gln Ala Asp Lys Ala Asp Ser 180 185 190Glu Ser Asn Gly Glu 1955484PRTHomo sapiensmisc_featureIncyte Clone No 2763296 54Met Thr Pro Gln Ser Leu Leu Gln Thr Thr Leu Phe Leu Leu Ser Leu1 5 10 15Leu Phe Leu Val Gln Gly Ala His Gly Arg Gly His Arg Glu Asp Phe 20 25 30Arg Phe Cys Ser Gln Arg Asn Gln Thr His Arg Ser Ser Leu His Tyr 35 40 45Tyr Trp Ser Met Arg Leu Gln Ala Arg Gly Gly Pro Ser Pro Leu Lys 50 55 60Ser Asn Ser Asp Ser Ala Arg Leu Pro Ile Ser Ser Gly Ser Thr Ser65 70 75 80Ser Ser Arg Ile5597PRTHomo sapiensmisc_featureIncyte Clone No 2779436 55Met Gln Leu Gly Thr Gly Leu Leu Leu Ala Ala Val Leu Ser Leu Gln1 5 10 15Leu Ala Ala Ala Glu Ala Ile Trp Cys His Gln Cys Thr Gly Phe Gly 20 25 30Gly Cys Ser His Gly Ser Arg Cys Leu Arg Asp Ser Thr His Cys Val 35 40 45Thr Thr Ala Thr Arg Val Leu Ser Asn Thr Glu Asp Leu Pro Leu Val 50 55 60Thr Lys Met Cys His Ile Gly Cys Pro Asp Ile Pro Ser Leu Gly Leu65 70 75 80Gly Pro Tyr Val Ser Ile Ala Cys Cys Gln Thr Ser Leu Cys Asn His 85 90 95Asp56140PRTHomo sapiensmisc_featureIncyte Clone No 2808528 56Met Ala Ala Ser Leu Gly Gln Val Leu Ala Leu Val Leu Val Ala Ala1 5 10 15Leu Trp Gly Gly Thr Gln Pro Leu Leu Lys Arg Ala Ser Ala Gly Leu 20 25 30Gln Arg Val His Glu Pro Thr Trp Ala Gln Gln Leu Leu Gln Glu Met 35 40 45Lys Thr Leu Phe Leu Asn Thr Glu Tyr Leu Met Pro Phe Leu Leu Asn 50 55 60Gln Cys Gly Ser Leu Leu Tyr Tyr Leu Thr Leu Ala Ser Thr Asp Leu65 70 75 80Thr Leu Ala Val Pro Ile Cys Asn Ser Leu Ala Ile Ile Phe Thr Leu 85 90 95Ile Val Gly Lys Ala Leu Gly Glu Asp Ile Gly Gly Lys Arg Ala Val 100 105 110Ala Gly Met Val Leu Thr Val Ile Gly Ile Ser Leu Cys Ile Thr Ser 115 120 125Ser Val Ser Lys Thr Gln Gly Gln Gln Ser Thr Leu 130 135 14057285PRTHomo sapiensmisc_featureIncyte Clone No 2809230 57Met Glu Val Pro Pro Pro Ala Pro Arg Ser Phe Leu Cys Arg Ala Leu1 5 10 15Cys Leu Phe Pro Arg Val Phe Ala Ala Glu Ala Val Thr Ala Asp Ser 20 25 30Glu Val Leu Glu Glu Arg Gln Lys Arg Leu Pro Tyr Val Pro Glu Pro 35 40 45Tyr Tyr Pro Glu Ser Gly Trp Asp Arg Leu Arg Glu Leu Phe Gly Lys 50 55 60Asp Glu Gln Gln Arg Ile Ser Lys Asp Leu Ala Asn Ile Cys Lys Thr65 70 75 80Ala Ala Thr Ala Gly Ile Ile Gly Trp Val Tyr Gly Gly Ile Pro Ala 85 90 95Phe Ile His Ala Lys Gln Gln Tyr Ile Glu Gln Ser Gln Ala Glu Ile 100 105 110Tyr His Asn Arg Phe Asp Ala Val Gln Ser Ala His Arg Ala Ala Thr 115 120 125Arg Gly Phe Ile Arg Tyr Gly Trp Arg Trp Gly Trp Arg Thr Ala Val 130 135 140Phe Val Thr Ile Phe Asn Thr Val Asn Thr Ser Leu Asn Val Tyr Arg145 150 155 160Asn Lys Asp Ala Leu Ser His Phe Val Ile Ala Gly Ala Val Thr Gly 165 170 175Ser Leu Phe Arg Ile Asn Val Gly Leu Arg Gly Leu Val Ala Gly Gly 180 185 190Ile Ile Gly Ala Leu Leu Gly Thr Pro Val Gly Gly Leu Leu Met Ala 195 200 205Phe Gln Lys Tyr Ser Gly Glu Thr Val Gln Glu Arg Lys Gln Lys Asp 210 215 220Arg Lys Ala Leu His Glu Leu Lys Leu Glu Glu Trp Lys Gly Arg Leu225 230 235 240Gln Val Thr Glu His Leu Pro Glu Lys Ile Glu Ser Ser Leu Gln Glu 245 250 255Asp Glu Pro Glu Asn Asp Ala Lys Lys Ile Glu Ala Leu Leu Asn Leu 260 265 270Pro Arg Asn Pro Ser Val Ile Asp Lys Gln Asp Lys Asp 275 280 28558262PRTHomo sapiensmisc_featureIncyte Clone No 2816821 58Met Thr Gln Pro Val Pro Arg Leu Ser Val Pro Ala Ala Leu Ala Leu1 5 10 15Gly Ser Ala Ala Leu Gly Ala Ala Phe Ala Thr Gly Leu Phe Leu Gly 20 25 30Arg Arg Cys Pro Pro Trp Arg Gly Arg Arg Glu Gln Cys Leu Leu Pro 35 40 45Pro Glu Asp Ser Arg Leu Trp Gln Tyr Leu Leu Ser Arg Ser Met Arg 50 55 60Glu His Pro Ala Leu Arg Ser Leu Arg Leu Leu Thr Leu Glu Gln Pro65 70 75 80Gln Gly Asp Ser Met Met Thr Cys Glu Gln Ala Gln Leu Leu Ala Asn 85 90 95Leu Ala Arg Leu Ile Gln Ala Lys Lys Ala Leu Asp Leu Gly Thr Phe 100 105 110Thr Gly Tyr Ser Ala Leu Ala Leu Ala Leu Ala Leu Pro Ala Asp Gly 115 120 125Arg Val Val Thr Cys Glu Val Asp Ala Gln Pro Pro Glu Leu Gly Arg 130 135 140Pro Leu Trp Arg Gln Ala Glu Ala Glu His Lys Ile Asp Leu Arg Leu145 150 155 160Lys Pro Ala Leu Glu Thr Leu Asp Glu Leu Leu Ala Ala Gly Glu Ala 165 170 175Gly Thr Phe Asp Val Ala Val Val Asp Ala Asp Lys Glu Asn Cys Ser 180 185 190Ala Tyr Tyr Glu Arg Cys Leu Gln Leu Leu Arg Pro Gly Gly Ile Leu 195 200 205Ala Val Leu Arg Val Leu Trp Arg Gly Lys Val Leu Gln Pro Pro Lys 210 215 220Gly Asp Val Ala Ala Glu Cys Val Arg Asn Leu Asn Glu Arg Ile Arg225 230 235 240Arg Asp Val Arg Val Tyr Ile Ser Leu Leu Pro Leu Gly Asp Gly Leu 245 250 255Thr Leu Ala Phe Lys Ile 26059189PRTHomo sapiensmisc_featureIncyte Clone No 2817268 59Met Ala Leu Leu Ser Arg Pro Ala Leu Thr Leu Leu Leu Leu Leu Met1 5 10 15Ala Ala Val Val Arg Cys Gln Glu Gln Ala Gln Thr Thr Asp Trp Arg 20 25 30Ala Thr Leu Lys Thr Ile Arg Asn Gly Val His Lys Ile Asp Thr Tyr 35 40 45Leu Asn Ala Ala Leu Asp Leu Leu Gly Gly Glu Asp Gly Leu Cys Gln 50 55 60Tyr Lys Cys Ser Asp Gly Ser Lys Pro Phe Pro Arg Tyr Gly Tyr Lys65 70 75 80Pro Ser Pro Pro Asn Gly Cys Gly Ser Pro Leu Phe Gly Val His Leu 85 90 95Asn Ile Gly Ile Pro Ser Leu Thr Lys Cys Cys Asn Gln His Asp Arg 100 105 110Cys Tyr Glu Thr Cys Gly Lys Ser Lys Asn Asp Cys Asp Glu Glu Phe 115 120 125Gln Tyr Cys Leu Ser Lys Ile Cys Arg Asp Val Gln Lys Thr Leu Gly 130 135 140Leu Thr Gln His Val Gln Ala Cys Glu Thr Thr Val Glu Leu Leu Phe145 150 155 160Asp Ser Val Ile His Leu Gly Cys Lys Pro Tyr Leu Asp Ser Gln Arg 165 170 175Ala Ala Cys Arg Cys His Tyr Glu Glu Lys Thr Asp Leu 180 18560257PRTHomo sapiensmisc_featureIncyte Clone No 2923165 60Met Thr Ala Ala Val Phe Phe Gly Cys Ala Phe Ile Ala Phe Gly Pro1 5 10 15Ala Leu Ala Leu Tyr Val Phe Thr Ile Ala Thr Glu Pro Leu Arg Ile 20 25 30Ile Phe Leu Ile Ala Gly Ala Phe Phe Trp Leu Val Ser Leu Leu Ile 35 40 45Ser Ser Leu Val Trp Phe Met Ala Arg Val Ile Ile Asp Asn Lys Asp 50 55 60Gly Pro Thr Gln Lys Tyr Leu Leu Ile Phe Gly Ala Phe Val Ser Val65 70 75 80Tyr Ile Gln Glu Met Phe Arg Phe Ala Tyr Tyr Lys Leu Leu Lys Lys 85 90 95Ala Ser Glu Gly Leu Lys Ser Ile Asn Pro Gly Glu Thr Ala Pro Ser 100 105 110Met Arg Leu Leu Ala Tyr Val Ser Gly Leu Gly Phe Gly Ile Met Ser 115 120 125Gly Val Phe Ser Phe Val Asn Thr Leu Ser Asp Ser Leu Gly Pro Gly 130 135 140Thr Val Gly Ile His Gly Asp Ser Pro Gln Phe Phe Leu Tyr Ser Ala145 150 155 160Phe Met Thr Leu Val Ile Ile Leu Leu His Val Phe Trp Gly Ile Val 165 170 175Phe Phe Asp Gly Cys Glu Lys Lys Lys Trp Gly Ile Leu Leu Ile Val 180 185 190Leu Leu Thr His Leu Leu Val Ser Ala Gln Thr Phe Ile Ser Ser Tyr 195 200 205Tyr Gly Ile Asn Leu Ala Ser Ala Phe Ile Ile Leu Val Leu Met Gly 210 215 220Thr Trp Ala Phe Leu Ala Ala Gly Gly Ser Cys Arg Ser Leu Lys Leu225 230 235 240Cys Leu Leu Cys Gln Asp Lys Asn Phe Leu Leu Tyr Asn Gln Arg Ser 245 250 255Arg6182PRTHomo sapiensmisc_featureIncyte Clone No 2949822 61Met Pro Phe Ser Trp Met Val Ile Ile Leu Gly Phe Leu Cys Gly Leu1 5 10 15Ser Gly Gln Leu Gln Ile Met Asn Thr Leu Ser Ser Leu Pro Ile Val 20 25 30Leu Leu Val Ser Ser Ser Cys Leu Ile Leu Ala Arg Met Ser Tyr Ser 35 40 45Ile Leu Thr Ser Ser Tyr Gly Gly Gly Val Phe Ile Leu Leu Asp Leu 50 55 60Lys Arg Asn Thr Ser Lys Val Ser Pro Leu Met Met Met Phe Ala Ile65 70 75 80Gly His62202PRTHomo sapiensmisc_featureIncyte Clone No 2992192 62Met Ala Ala Pro Trp Arg Arg Trp Pro Thr Gly Leu Leu Ala Val Leu1 5 10 15Arg Pro Leu Leu Thr Cys Arg Pro Leu Gln Gly Thr Thr Leu Gln Arg 20 25 30Asp Val Leu Leu Phe Glu His Asp Arg Gly Arg Phe Phe Thr Ile Leu 35 40 45Gly Leu Phe Cys Ala Gly Gln Gly Val Phe Trp Ala Ser Met Ala Val 50 55 60Ala Ala Val Ser Arg Pro Pro Val Pro Val Gln Pro Leu Asp Ala Glu65 70 75 80Val Pro Asn Arg Gly Pro Phe Asp Leu Arg Ser Ala Leu Trp Arg Tyr 85 90 95Gly Leu Ala Val Gly Cys Gly Ala Ile Gly Ala Leu Val Leu Gly Ala 100 105 110Gly Leu Leu Phe Ser Leu Arg Ser Val Arg Ser Val Val Leu Arg Ala 115 120 125Gly Gly Gln Gln Val Thr Leu Thr Thr His Ala Pro Phe Gly Leu Gly 130 135 140Ala His Phe Thr Val Pro Leu Lys Gln Val Ser Cys Met Ala His Arg145 150 155 160Gly Glu Val Pro Ala Met Leu Pro Leu Lys Val Lys Gly Arg Arg Phe 165 170 175Tyr Phe Leu Leu Asp Lys Thr Gly His Phe Pro Asn Thr Lys Leu Phe 180 185 190Asp Asn Thr Val Gly Ala Tyr Arg Ser Leu 195 20063450PRTHomo sapiensmisc_featureIncyte Clone No 2992458 63Met Leu Val Thr Ala Tyr Leu Ala Phe Val Gly Leu Leu Ala Ser Cys1 5 10 15Leu Gly Leu Glu Leu Ser Arg Cys Arg Ala Lys Pro Pro Gly Arg Ala 20 25 30Cys Ser Asn Pro Ser Phe Leu Arg Phe Gln Leu Asp Phe Tyr Gln Val 35 40 45Tyr Phe Leu Ala Leu Ala Ala Asp Trp Leu Gln Ala Pro Tyr Leu Tyr 50 55 60Lys Leu Tyr Gln His Tyr Tyr Phe Leu Glu Gly Gln Ile Ala Ile Leu65 70 75 80Tyr Val Cys Gly Leu Ala Ser Thr Val Leu Phe Gly Leu Val Ala Ser 85 90 95Ser Leu Val Asp Trp Leu Gly Arg Lys Asn Ser Cys Val Leu Phe Ser 100 105 110Leu Thr Tyr Ser Leu Cys Cys Leu Thr Lys Leu Ser Gln Asp Tyr Phe 115 120 125Val Leu Leu Val Gly Arg Ala Leu Gly Gly Leu Ser Thr Ala Leu Leu 130 135 140Phe Ser Ala Phe Glu Ala Trp Tyr Ile His Glu His Val Glu Arg His145 150 155 160Asp Phe Pro Ala Glu Trp Ile Pro Ala Thr Phe Ala Arg Ala Ala Phe 165 170 175Trp Asn His Val Leu Ala Val Val Ala Gly Val Ala Ala Glu Ala Val 180 185 190Ala Ser Trp Ile Gly Leu Gly Pro Val Ala Pro Phe Val Ala Ala Ile 195 200 205Pro Leu Leu Ala Leu Ala Gly Ala Leu Ala Leu Arg Asn Trp Gly Glu 210 215 220Asn Tyr Asp Arg Gln Arg Ala Phe Ser Arg Thr Cys Ala Gly Gly Leu225 230 235 240Arg Cys Leu Leu Ser Asp Arg Arg Val Leu Leu Leu Gly Thr Ile Gln 245 250 255Ala Leu Phe Glu Ser Val Ile Phe Ile Phe Val Phe Leu Trp Thr Pro 260 265 270Val Leu Asp Pro His Gly Ala Pro Leu Gly Ile Ile Phe Ser Ser Phe 275 280 285Met Ala Ala Ser Leu Leu Gly Ser Ser Leu Tyr Arg Ile Ala Thr Ser 290 295 300Lys Arg Tyr His Leu Gln Pro Met His Leu Leu Ser Leu Ala Val Leu305 310 315 320Ile Val Val Phe Ser Leu Phe Met Leu Thr Phe Ser Thr Ser Pro Gly 325 330 335Gln Glu Ser Pro Val Glu Ser Phe Ile Ala Phe Leu Leu Ile Glu Leu 340 345 350Ala Cys Gly Leu Tyr Phe Pro Ser Met Ser Phe Leu Arg Arg Lys Val 355 360 365Ile Pro Glu Thr Glu Gln Ala Gly Val Leu Asn Trp Phe Arg Val Pro 370 375 380Leu His Ser Leu Ala Cys Leu Gly Leu Leu Val Leu His Asp Ser Asp385 390 395 400Arg Lys Thr Gly Thr Arg Asn Met Phe Ser Ile Cys Ser Ala Val Met 405 410 415Val Met Ala Leu Leu Ala Val Val Gly Leu Phe Thr Val Val Arg His 420 425 430Asp Ala Glu Leu Arg Val Pro Ser Pro Thr Glu Glu Pro Tyr Ala Pro 435 440 445Glu Leu 45064322PRTHomo sapiensmisc_featureIncyte Clone No 3044710 64Met Ala Arg Cys Phe Ser Leu Val Leu Leu Leu Thr Ser Ile Trp Thr1 5 10 15Thr Arg Leu Leu Val Gln Gly Ser Leu Arg Ala Glu Glu Leu Ser Ile 20 25 30Gln Val Ser Cys Arg Ile Met Gly Ile Thr Leu Val Ser Lys Lys Ala 35 40 45Asn Gln Gln Leu Asn Phe Thr Glu Ala Lys Glu Ala Cys Arg Leu Leu 50 55 60Gly Leu Ser Leu Ala Gly Lys Asp Gln Val Glu Thr Ala Leu Lys Ala65 70 75 80Ser Phe Glu Thr Cys Ser Tyr Gly Trp Val Gly Asp Gly Phe Val Val 85 90 95Ile Ser Arg Ile Ser Pro Asn Pro Lys Cys Gly Lys Asn Gly Val Gly 100 105 110Val Leu Ile Trp Lys Val Pro Val Ser Arg Gln Phe Ala Ala Tyr Cys 115 120 125Tyr Asn Ser Ser Asp Thr Trp Thr Asn Ser Cys Ile Pro Glu Ile Ile 130 135 140Thr Thr Lys Asp Pro Ile Phe Asn Thr Gln Thr Ala Thr Gln Thr Thr145 150 155 160Glu Phe Ile Val Ser Asp Ser Thr Tyr Ser Val Ala Ser Pro Tyr Ser 165 170 175Thr Ile Pro Ala Pro Thr Thr Thr Pro Pro Ala Pro Ala Ser Thr Ser 180 185 190Ile Pro Arg Arg Lys Lys Leu Ile Cys Val Thr Glu Val Phe Met Glu 195 200 205Thr Ser Thr Met Ser Thr Glu Thr Glu Pro Phe Val Glu Asn Lys Ala 210 215 220Ala Phe Lys Asn Glu Ala Ala Gly Phe Gly Gly Val Pro Thr Ala Leu225 230 235 240Leu Val

Leu Ala Leu Leu Phe Phe Gly Ala Ala Ala Gly Leu Gly Phe 245 250 255Cys Tyr Val Lys Arg Tyr Val Lys Ala Phe Pro Phe Thr Asn Lys Asn 260 265 270Gln Gln Lys Glu Met Ile Glu Thr Lys Val Val Lys Glu Glu Lys Ala 275 280 285Asn Asp Ser Asn Pro Asn Glu Glu Ser Lys Lys Thr Asp Lys Asn Pro 290 295 300Glu Glu Ser Lys Ser Pro Ser Lys Thr Thr Val Arg Cys Leu Glu Ala305 310 315 320Glu Val65104PRTHomo sapiensmisc_featureIncyte Clone No 3120415 65Met Lys Leu Ala Ala Leu Leu Gly Leu Cys Val Ala Leu Ser Cys Ser1 5 10 15Ser Ala Ala Ala Phe Leu Val Gly Ser Ala Lys Pro Val Ala Gln Pro 20 25 30Val Ala Ala Leu Glu Ser Ala Ala Glu Ala Gly Ala Gly Thr Leu Ala 35 40 45Asn Pro Leu Gly Thr Leu Asn Pro Leu Lys Leu Leu Leu Ser Ser Leu 50 55 60Gly Ile Pro Val Asn His Leu Ile Glu Gly Ser Gln Lys Cys Val Ala65 70 75 80Glu Leu Gly Pro Gln Ala Val Gly Ala Val Lys Ala Leu Lys Ala Leu 85 90 95Leu Gly Ala Leu Thr Val Phe Gly 1006693PRTHomo sapiensmisc_featureIncyte Clone No 126758 66Met Lys Leu Val Thr Ile Phe Leu Leu Val Thr Ile Ser Leu Cys Ser1 5 10 15Tyr Ser Ala Thr Ala Phe Leu Ile Asn Lys Val Pro Leu Pro Val Asp 20 25 30Lys Leu Ala Pro Leu Pro Leu Asp Asn Ile Leu Pro Phe Met Asp Pro 35 40 45Leu Lys Leu Leu Leu Lys Thr Leu Gly Ile Ser Val Glu His Leu Val 50 55 60Glu Gly Leu Arg Lys Cys Val Asn Glu Leu Gly Pro Glu Ala Ser Glu65 70 75 80Ala Val Lys Lys Leu Leu Glu Ala Leu Ser His Leu Val 85 906771PRTHomo sapiensmisc_featureIncyte Clone No 674760 67Met Thr Ala Gly Gln Phe Pro Ala Leu Val Ser Leu Ala Leu Leu Leu1 5 10 15Asp Gly Gly Arg Arg Ala Ser Ala Arg Arg Asn Arg Gly His Leu Trp 20 25 30Val Phe Cys Thr Ser Phe Leu Leu Ala Pro Trp Glu Val Glu Asp Val 35 40 45Gly Trp Lys Lys Gly Leu Asp Leu Pro Pro Ser Ser Ser Pro Pro Ser 50 55 60Pro Lys Glu Leu Ala Leu Gln65 7068394PRTHomo sapiensmisc_featureIncyte Clone No 1229438 68Met Lys Arg Gln Asn Val Arg Thr Leu Ala Leu Ile Val Cys Thr Phe1 5 10 15Thr Tyr Leu Leu Val Gly Ala Ala Val Phe Asp Ala Leu Glu Ser Glu 20 25 30Pro Glu Leu Ile Glu Arg Gln Arg Leu Glu Leu Arg Gln Gln Glu Leu 35 40 45Arg Ala Arg Tyr Asn Leu Ser Gln Gly Gly Tyr Glu Glu Leu Glu Arg 50 55 60Val Val Leu Arg Leu Lys Pro His Lys Ala Gly Val Gln Trp Arg Phe65 70 75 80Ala Gly Ser Phe Tyr Phe Ala Ile Thr Val Ile Thr Thr Ile Gly Tyr 85 90 95Gly His Ala Ala Pro Ser Thr Asp Gly Gly Lys Val Phe Cys Met Phe 100 105 110Tyr Ala Leu Leu Gly Ile Pro Leu Thr Leu Val Met Phe Gln Ser Leu 115 120 125Gly Glu Arg Ile Asn Thr Leu Val Arg Tyr Leu Leu His Arg Ala Lys 130 135 140Lys Gly Leu Gly Met Arg Arg Ala Asp Val Ser Met Ala Asn Met Val145 150 155 160Leu Ile Gly Phe Phe Ser Cys Ile Ser Thr Leu Cys Ile Gly Ala Ala 165 170 175Ala Phe Ser His Tyr Glu His Trp Thr Phe Phe Gln Ala Tyr Tyr Tyr 180 185 190Cys Phe Ile Thr Leu Thr Thr Ile Gly Phe Gly Asp Tyr Val Ala Leu 195 200 205Gln Lys Asp Gln Ala Leu Gln Thr Gln Pro Gln Tyr Val Ala Phe Ser 210 215 220Phe Val Tyr Ile Leu Thr Gly Leu Thr Val Ile Gly Ala Phe Leu Asn225 230 235 240Leu Val Val Leu Arg Phe Met Thr Met Asn Ala Glu Asp Glu Lys Arg 245 250 255Asp Ala Glu His Arg Ala Leu Leu Thr Arg Asn Gly Gln Ala Gly Gly 260 265 270Gly Gly Gly Gly Gly Ser Ala His Thr Thr Asp Thr Ala Ser Ser Thr 275 280 285Ala Ala Ala Gly Gly Gly Gly Phe Arg Asn Val Tyr Ala Glu Val Leu 290 295 300His Phe Gln Ser Met Cys Ser Cys Leu Trp Tyr Lys Ser Arg Glu Lys305 310 315 320Leu Gln Tyr Ser Ile Pro Met Ile Ile Pro Arg Asp Leu Ser Thr Ser 325 330 335Asp Thr Cys Val Glu Gln Ser His Ser Ser Pro Gly Gly Gly Gly Arg 340 345 350Tyr Ser Asp Thr Pro Ser Arg Arg Cys Leu Cys Ser Gly Ala Pro Arg 355 360 365Ser Ala Ile Ser Ser Val Ser Thr Gly Leu His Ser Leu Ser Thr Phe 370 375 380Arg Gly Leu Met Lys Arg Arg Ser Ser Val385 3906972PRTHomo sapiensmisc_featureIncyte Clone No 1236935 69Met Cys Pro Phe Phe Pro Leu Thr Ser Leu Ile Val Phe Leu Ile Leu1 5 10 15Phe Phe Lys Thr Ile Ala Ser Ser Gly Ser Gly Gly Ser Cys Leu Gly 20 25 30Leu Pro Lys Cys Trp Asp Tyr Arg Arg Glu His Arg Ala Arg Pro Thr 35 40 45Ile Val Phe Ser Lys His Val Tyr Thr Tyr Ser Met Arg Met Gln Ile 50 55 60Glu Ile Ser Thr Asn Ile Ser Gln65 707071PRTHomo sapiensmisc_featureIncyte Clone No 1359283 70Met Arg Leu Thr Gly Leu Thr Leu Leu Leu Ser Leu Met Glu Ser Leu1 5 10 15Gly Gln Val Glu Asp Arg Phe Phe Ser Thr His Arg Arg Phe Pro His 20 25 30His Thr Pro Ile Ser Gly Leu Leu Cys Arg Glu Phe Ser Leu Pro Lys 35 40 45Arg Ser Gly Val Pro Trp Thr Arg Val Leu Ile Ser Cys Ile Trp Arg 50 55 60Ser Gly Ala Gly Lys Arg Met65 7071247PRTHomo sapiensmisc_featureIncyte Clone No 1450703 71Met His Leu Ala Arg Leu Val Gly Ser Cys Ser Leu Leu Leu Leu Leu1 5 10 15Gly Ala Leu Ser Gly Trp Ala Ala Ser Asp Asp Pro Ile Glu Lys Val 20 25 30Ile Glu Gly Ile Asn Arg Gly Leu Ser Asn Ala Glu Arg Glu Val Gly 35 40 45Lys Ala Leu Asp Gly Ile Asn Ser Gly Ile Thr His Ala Gly Arg Glu 50 55 60Val Glu Lys Val Phe Asn Gly Leu Ser Asn Met Gly Ser His Thr Gly65 70 75 80Lys Glu Leu Asp Lys Gly Val Gln Gly Leu Asn His Gly Met Asp Lys 85 90 95Val Ala His Glu Ile Asn His Gly Ile Gly Gln Ala Gly Lys Glu Ala 100 105 110Glu Lys Leu Gly His Gly Val Asn Asn Ala Ala Gly Gln Ala Gly Lys 115 120 125Glu Ala Asp Lys Ala Val Gln Gly Phe His Thr Gly Val His Gln Ala 130 135 140Gly Lys Glu Ala Glu Lys Leu Gly Gln Gly Val Asn His Ala Ala Asp145 150 155 160Gln Ala Gly Lys Glu Val Glu Lys Leu Gly Gln Gly Ala His His Ala 165 170 175Ala Gly Gln Ala Gly Lys Glu Leu Gln Asn Ala His Asn Gly Val Asn 180 185 190Gln Ala Ser Lys Glu Ala Asn Gln Leu Leu Asn Gly Asn His Gln Ser 195 200 205Gly Ser Ser Ser His Gln Gly Gly Ala Thr Thr Thr Pro Leu Ala Ser 210 215 220Gly Ala Ser Val Asn Thr Pro Phe Ile Asn Leu Pro Ala Leu Trp Arg225 230 235 240Ser Val Ala Asn Ile Met Pro 2457273PRTHomo sapiensmisc_featureIncyte Clone No 1910668 72Met Thr Cys Trp Met Leu Pro Pro Ile Ser Phe Leu Ser Tyr Leu Pro1 5 10 15Leu Trp Leu Gly Pro Ile Trp Pro Cys Ser Gly Ser Thr Leu Gly Lys 20 25 30Pro Asp Pro Gly Val Trp Pro Ser Leu Phe Arg Pro Trp Asp Ala Ala 35 40 45Ser Pro Gly Asn Tyr Ala Leu Ser Arg Gly Glu Asn Gln Tyr Glu Lys 50 55 60Trp Gly Gln Gly Thr His Ser Ser Leu65 707370PRTHomo sapiensmisc_featureIncyte Clone No 1955143 73Met Gly Arg Leu Arg Tyr Phe Phe Ser Leu Leu Leu Leu Arg Trp Gly1 5 10 15Gln Leu Leu Gly Ala Asp Glu Phe Cys Cys His Lys Ser Tyr Ile Ala 20 25 30His Leu Val Cys Thr Glu Ser Ala Ile Leu Asn Pro Gly His Ala Leu 35 40 45Glu Leu Tyr Lys Lys Asn Leu Gln Val Ser Ile Leu Ser Pro Tyr Pro 50 55 60Thr Asp Pro Ile His Leu65 707467PRTHomo sapiensmisc_featureIncyte Clone No 1961637 74Met Met Phe Thr Ser Leu Ser Leu Ala Leu Pro Phe Leu Leu Gln Thr1 5 10 15Met Leu Cys Leu Arg Ala Leu Leu Ile Ala Val Pro His Gly His Asp 20 25 30Trp Asn Arg Asp Ala Thr Ser Phe Tyr Thr Ser Thr Val Ser Trp Val 35 40 45Lys Ser Phe Phe Leu Phe Val Leu Asp Gly Val Ser Leu Leu Leu Pro 50 55 60Arg Leu Glu657591PRTHomo sapiensmisc_featureIncyte Clone No 1990762 75Met Trp Pro Thr Thr Trp Ala Trp Ser Trp Val Gln Thr Leu Thr Leu1 5 10 15Ala Leu Leu Ile Ser Cys Val Thr Leu Gly Gln Leu Ile Thr Thr Leu 20 25 30Gln Val Ser Phe Leu Ile Cys Glu Met Asp Val Ile Ile Gly Cys Asp 35 40 45Glu Met Ile Pro Ser Glu Ser Leu Val Leu Leu Trp Pro Pro Pro Leu 50 55 60Leu Leu Leu Gly Glu Phe Trp Ile Trp Asn Pro Val Ser Arg Ile Leu65 70 75 80Phe Trp Leu Cys His Val Pro Ala Gly Gln Leu 85 907656PRTHomo sapiensmisc_featureIncyte Clone No 1994131 76Met Asn Glu Trp Trp Leu Leu Leu Leu Leu His Leu His Pro Pro Arg1 5 10 15Val Ile Ser Pro Phe Trp Phe Ile Val Ser Val Leu Thr Ala Cys Asp 20 25 30Asn Arg Lys Tyr Ile Leu Leu Arg Thr Val Pro Val Phe Ser Phe Pro 35 40 45Glu Asn Thr Tyr Phe Asp Val Gly 50 5577112PRTHomo sapiensmisc_featureIncyte Clone No 1997745 77Met Pro Leu Phe Leu Ser Ile Pro Ser Leu Phe Leu Thr Leu Ser Gly1 5 10 15Leu Gly Leu Ala Val Gln Ser Pro Ala Gly Gly Cys Trp Gly Leu Ser 20 25 30Leu Cys Arg His Cys Val Phe Leu Arg Gly Cys Pro Gln Asn Thr Pro 35 40 45Pro Ala Pro Trp Gly Ser Ser Gly Ser His Phe Ser Trp Ser Leu Arg 50 55 60Ser Gln Lys Gln Leu Leu Gln Glu Ala Lys Lys Arg Leu Gly Trp Leu65 70 75 80Leu Val Leu Met Met Ala Phe Ile Leu Leu Gly His Phe Gly Tyr Ile 85 90 95His Gly His Cys Phe His Leu Ser Phe Leu Pro Val Pro Pro Leu Pro 100 105 1107854PRTHomo sapiensmisc_featureIncyte Clone No 2009035 78Met Met Leu Gln Pro Val Asp Leu Leu Gln Ser Tyr Leu Leu Leu Leu1 5 10 15Tyr Cys Trp Ser Phe Ser Leu Leu Phe Thr Leu Leu Cys Asn Ala Val 20 25 30Arg Asn Asp Phe Phe His Lys Leu Phe Ser Ile Tyr Trp Met Tyr Asn 35 40 45Leu Thr His Ser Lys His 507957PRTHomo sapiensmisc_featureIncyte Clone No 2009152 79Met Lys Phe Tyr Ala Val Leu Leu Ser Ile Cys Leu Leu Leu Ser Cys1 5 10 15Trp Cys Ala Cys His Val Arg Asp Cys Asn Leu Ile Cys Leu Phe Ser 20 25 30Thr Val Lys Ala Ile Thr Arg Glu Leu Leu Gln Leu Pro Ser Tyr Val 35 40 45Lys Arg Phe Phe Phe Asn Ser Leu Arg 50 558052PRTHomo sapiensmisc_featureIncyte Clone No 2061752 80Met Gln Arg Leu Gly Lys Ala Pro Gly Thr Trp Gln Ala Ile Ser Lys1 5 10 15Cys Trp Leu Leu Leu Leu Leu Ser Leu Pro Phe Ser Gln Ser Ile Ile 20 25 30Ile Ser Leu Arg Ala Gly Thr Met Ser Tyr Leu Pro Leu Tyr Phe Pro 35 40 45Gln Tyr Phe Pro 508164PRTHomo sapiensmisc_featureIncyte Clone No 2061933 81Met Lys Leu Leu Leu Leu Lys Leu Asp Phe Phe Ile Leu Leu Gly Ser1 5 10 15Glu Glu Ser Arg Cys Leu Val Asp Val Gln Tyr Val Ile Phe Phe Leu 20 25 30Ile Glu Cys Val His Leu Lys Ser Ser Leu Thr Phe Leu Glu Arg Leu 35 40 45Leu Ser Ile Asn Asn Gly Ile Leu Glu Glu Lys Trp Phe Phe Lys Ser 50 55 608265PRTHomo sapiensmisc_featureIncyte Clone No 2081422 82Met Lys Pro Leu Ile Pro Phe Leu Ser Pro Pro Pro Leu Leu Pro Leu1 5 10 15Thr Phe Phe Leu Ser Ser Leu Leu Leu Ser Pro Leu Cys Arg Ala Leu 20 25 30Gly Thr Ser Gln Ala Val Pro Pro Leu Arg Ala Leu Ser Val Thr Asp 35 40 45Ala His Gly Ser Leu Leu Leu His Pro Lys Thr Leu Ala Cys Pro Cys 50 55 60Leu658356PRTHomo sapiensmisc_featureIncyte Clone No 2101278 83Met Arg Ala Asp Arg Leu Leu Pro Ile Ser Ala Leu Cys Leu Leu Tyr1 5 10 15Thr Pro Gly Gly Ala Leu Glu Pro Ala Gln Val Gly Tyr Thr Ile Phe 20 25 30Leu Asn Ser Ile Trp Leu Pro Ala Tyr Phe Phe His Leu Phe Thr Val 35 40 45Ile Ser Gly Val Phe Leu Phe Ile 50 5584120PRTHomo sapiensmisc_featureIncyte Clone No 2121353 84Met Pro Ala Leu Pro Pro Gly Phe Ser Gln Ala Gly Ser Cys Val Pro1 5 10 15Thr Gly Ser Ser Leu Val Leu Cys Leu Leu Ala Ala Ser Leu Leu Leu 20 25 30Phe Val Pro Thr Leu Ala Leu Leu Thr Gly Ala Thr Thr Cys Trp Cys 35 40 45Leu His Asn Lys Arg Leu Ala Leu Arg Pro Leu Ala Trp Gln Gly Leu 50 55 60Trp Gly Leu Val Ser Thr Arg Leu Ser His Gly Arg Thr Ser Phe Tyr65 70 75 80Phe Asn Ser Leu Pro Leu Gln Thr Asn Ser Ser Thr Cys Gln Asn His 85 90 95Ser Trp Asp Ser Gly Ala Arg Ala Thr Ala Leu Ala Ser Gly Arg Thr 100 105 110Gln Glu Gly Gly Val Gly Ser Val 115 1208567PRTHomo sapiensmisc_featureIncyte Clone No 2241736 85Met Asn Ser Leu Val Leu Phe Leu Gly His Leu Gly Leu Leu Ile Lys1 5 10 15Asp Cys Val Leu Leu Phe Ala Met Ser Lys Val Ser Gln Lys Gln Lys 20 25 30Val Leu Gly Pro Phe Gly Ser Pro Glu Leu Glu Ser Leu Gly Ile Gly 35 40 45Pro Arg Tyr Leu His Phe His Arg Phe Leu Val Gly Asp Phe Leu Gln 50 55 60Ala Lys Val658662PRTHomo sapiensmisc_featureIncyte Clone No 2271935 86Met Ala Trp Leu Ser Phe Ala Ala Val Glu Met Thr Leu Leu Leu His1 5 10 15Ser Ser Ser Leu Leu Ser Phe Ala Lys Val Val Leu Ser Leu Pro Glu 20 25 30Ile Arg Pro Phe Gly Asp Gly Asn Phe Ser Leu Lys Gln Ser Ser Lys 35 40 45Gln Asn Pro Asn Pro Ala Arg Val Gly Arg Lys Ser Met Phe 50 55 608775PRTHomo sapiensmisc_featureIncyte Clone No 2295344 87Met Met Ile Leu Leu Ser Leu Leu Val Ala Leu Ile Ser Val Ser Leu1 5 10 15Val Phe Leu Gly Leu Val Arg Phe Ser Arg Glu Asp Phe Ser Phe Pro 20 25 30Leu Trp Arg Glu Lys Ala Phe Tyr Gln His Ser Ser Ser Ser Val Gly 35 40 45Glu Arg Leu Gln Ala Leu Arg Lys His Ala Phe Thr Leu Phe Gly Thr 50 55 60Ile Pro Leu Leu Val Thr Val Pro Gln Val Pro65 70 758880PRTHomo sapiensmisc_featureIncyte Clone No 2303994 88Met Asn Ser Ile Phe Phe Leu Ser Leu Cys Leu Pro Leu Trp Val Ser1 5 10 15Leu Leu Trp Ala Lys Pro Leu Glu Met His Lys Thr Ser Arg His Gly 20 25 30Phe Trp Gln Lys Leu His Asp Phe Lys Leu Ala Leu Leu Leu Leu Thr 35 40

45Phe His Arg Glu Lys Ile Phe Pro Leu Lys Lys Thr Gly Leu Val Ile 50 55 60Phe Ser Leu Val Ala Leu Ser Arg Asp Ile Ser Ala Leu His Tyr Thr65 70 75 808950PRTHomo sapiensmisc_featureIncyte Clone No 2497805 89Met Arg Pro Ala Arg Leu Gly Pro Arg Cys Ser Asp Leu Asp Phe Gly1 5 10 15Leu Val Leu Ser Ser Trp Leu Arg Leu Ala Arg Cys Pro Leu Glu Ser 20 25 30Ser Phe Gly Phe Ala Phe Phe Val Cys Leu Phe Ser Pro Asn Phe Cys 35 40 45Gln Thr 5090116PRTHomo sapiensmisc_featureIncyte Clone No 2646362 90Met Trp Trp Ala Leu Cys Ser Met Leu Pro Leu Leu Gly Cys Ala Cys1 5 10 15Ser Ser Gly Cys Trp Gly Ser Gly Pro Thr Pro Leu Leu Ala Glu Pro 20 25 30Thr Phe Leu Cys Val Ser Ser Arg Pro His Asn Pro Leu Ser Phe Leu 35 40 45Ser Val Leu Pro Cys Ser Arg Gly Pro Gly Pro Ser Gly Leu Gln Gly 50 55 60Asp Gly Ala Gly Leu Pro Ala His Leu Gly Pro Leu Ser Cys Ile Cys65 70 75 80Leu Pro Ser Leu Leu Cys Asp Leu Gly Glu Arg Gln Cys Pro Leu Trp 85 90 95Ala Val Arg Ser Thr Gln Cys Leu Ile Ala Gly Lys Lys Val Leu Gln 100 105 110Arg Leu Cys Pro 1159167PRTHomo sapiensmisc_featureIncyte Clone No 2657146 91Met Ile Cys Gln Cys Leu Arg Leu Leu Leu Val Leu Val Thr Leu Leu1 5 10 15Ile Cys Phe Ser Pro Asp Arg Leu Thr Cys Pro Leu Asn Ser Ala Val 20 25 30Val Leu Ala Ser Tyr Ala Val Gln Cys Lys Ser Gln Arg Glu His Phe 35 40 45Thr Asp Gly Gln Val Val Leu Ile Ser Val Trp Arg Lys Ser Leu Val 50 55 60Pro Pro Ala6592538PRTHomo sapiensmisc_featureIncyte Clone No 2755786 92Met Ala Gly Ala Arg Ala Ala Ala Ala Ala Ala Ser Ala Gly Ser Ser1 5 10 15Ala Ser Ser Gly Asn Gln Pro Pro Gln Glu Leu Gly Leu Gly Glu Leu 20 25 30Leu Glu Glu Phe Ser Arg Thr Gln Tyr Arg Ala Lys Asp Gly Ser Gly 35 40 45Thr Gly Gly Ser Lys Val Glu Arg Ile Glu Lys Arg Cys Leu Glu Leu 50 55 60Phe Gly Arg Asp Tyr Cys Phe Ser Val Ile Pro Asn Thr Asn Gly Asp65 70 75 80Ile Cys Gly His Tyr Pro Arg His Ile Val Phe Leu Glu Tyr Glu Ser 85 90 95Ser Glu Lys Glu Lys Asp Thr Phe Glu Ser Thr Val Gln Val Ser Lys 100 105 110Leu Gln Asp Leu Ile His Arg Ser Lys Met Ala Arg Cys Arg Gly Arg 115 120 125Phe Val Cys Pro Val Ile Leu Phe Lys Gly Lys His Ile Cys Arg Ser 130 135 140Ala Thr Leu Ala Gly Trp Gly Glu Leu Tyr Gly Arg Ser Gly Tyr Asn145 150 155 160Tyr Phe Phe Ser Gly Gly Ala Asp Asp Ala Trp Ala Asp Val Glu Asp 165 170 175Val Thr Glu Glu Asp Cys Ala Leu Arg Ser Gly Asp Thr His Leu Phe 180 185 190Asp Lys Val Arg Gly Tyr Asp Ile Lys Leu Leu Arg Tyr Leu Ser Val 195 200 205Lys Tyr Ile Cys Asp Leu Met Val Glu Asn Lys Lys Val Lys Phe Gly 210 215 220Met Asn Val Thr Ser Ser Glu Lys Val Asp Lys Ala Gln Arg Tyr Ala225 230 235 240Asp Phe Thr Leu Leu Ser Ile Pro Tyr Pro Gly Cys Glu Phe Phe Lys 245 250 255Glu Tyr Lys Asp Arg Asp Tyr Met Ala Glu Gly Leu Ile Phe Asn Trp 260 265 270Lys Gln Asp Tyr Val Asp Ala Pro Leu Ser Ile Pro Asp Phe Leu Thr 275 280 285His Ser Leu Asn Ile Asp Trp Ser Gln Tyr Gln Cys Trp Asp Leu Val 290 295 300Gln Gln Thr Gln Asn Tyr Leu Lys Leu Leu Leu Ser Leu Val Asn Ser305 310 315 320Asp Asp Asp Ser Gly Leu Leu Val His Cys Ile Ser Gly Trp Asp Arg 325 330 335Thr Pro Leu Phe Ile Ser Leu Leu Arg Leu Ser Leu Trp Ala Asp Gly 340 345 350Leu Ile His Thr Ser Leu Lys Pro Thr Glu Ile Leu Tyr Leu Thr Val 355 360 365Ala Tyr Asp Trp Phe Leu Phe Gly His Met Leu Val Asp Arg Leu Ser 370 375 380Lys Gly Glu Glu Ile Phe Phe Phe Cys Phe Asn Phe Leu Lys His Ile385 390 395 400Thr Ser Glu Glu Phe Ser Ala Leu Lys Thr Gln Arg Arg Lys Ser Leu 405 410 415Pro Ala Arg Asp Gly Gly Phe Thr Leu Glu Asp Ile Cys Met Leu Arg 420 425 430Arg Lys Asp Arg Gly Ser Thr Thr Ser Leu Gly Ser Asp Phe Ser Leu 435 440 445Val Met Glu Ser Ser Pro Gly Ala Thr Gly Ser Phe Thr Tyr Glu Ala 450 455 460Val Glu Leu Val Pro Ala Gly Ala Pro Thr Gln Ala Ala Trp Leu Ala465 470 475 480Ala Leu Ser Asp Arg Glu Thr Arg Leu Gln Glu Val Arg Ser Ala Phe 485 490 495Leu Ala Ala Tyr Ser Ser Thr Val Gly Leu Arg Ala Val Ala Pro Ser 500 505 510Pro Ser Gly Ala Ile Gly Gly Leu Leu Glu Gln Phe Ala Arg Gly Val 515 520 525Gly Leu Arg Ser Ile Ser Ser Asn Ala Leu 530 5359358PRTHomo sapiensmisc_featureIncyte Clone No 2831245 93Met Glu Met Lys Gly Ser Arg Val Trp Leu Leu Leu Leu Phe Met Trp1 5 10 15Lys Ala Arg Pro Thr Phe Phe Gln Ser Cys Val Val Pro Phe Ile Leu 20 25 30Ser Pro Gln Asn Cys Val Gln Thr His Ser Leu Gly Pro Gly Val Trp 35 40 45Leu Gly Val Phe Pro Ser Gly Ser Leu His 50 5594119PRTHomo sapiensmisc_featureIncyte Clone No 3116250 94Met Lys Val Leu Ile Ser Ser Leu Leu Leu Leu Leu Pro Leu Met Leu1 5 10 15Met Ser Met Val Ser Ser Ser Leu Asn Pro Gly Val Ala Arg Gly His 20 25 30Arg Asp Arg Gly Gln Ala Ser Arg Arg Trp Leu Gln Glu Gly Gly Gln 35 40 45Glu Cys Glu Cys Lys Asp Trp Phe Leu Arg Ala Pro Arg Arg Lys Phe 50 55 60Met Thr Val Ser Gly Leu Pro Lys Lys Gln Cys Pro Cys Asp His Phe65 70 75 80Lys Gly Asn Val Lys Lys Thr Arg His Gln Arg His His Arg Lys Pro 85 90 95Asn Lys His Ser Arg Ala Cys Gln Gln Phe Leu Lys Gln Cys Gln Leu 100 105 110Arg Ser Phe Ala Leu Pro Leu 11595128PRTHomo sapiensmisc_featureIncyte Clone No 3129630 95Met Ala Tyr Ser Thr Val Gln Arg Val Ala Leu Ala Ser Gly Leu Val1 5 10 15Leu Ala Leu Ser Leu Leu Leu Pro Lys Ala Phe Leu Ser Arg Gly Lys 20 25 30Arg Gln Glu Pro Pro Pro Thr Pro Glu Gly Lys Leu Gly Arg Phe Pro 35 40 45Pro Met Met His His His Gln Ala Pro Ser Asp Gly Gln Thr Pro Gly 50 55 60Ala Arg Phe Gln Arg Ser His Leu Ala Glu Ala Phe Ala Lys Ala Lys65 70 75 80Gly Ser Gly Gly Gly Ala Gly Gly Gly Gly Ser Gly Arg Gly Leu Met 85 90 95Gly Gln Ile Ile Pro Ile Tyr Gly Phe Gly Ile Phe Leu Tyr Ile Leu 100 105 110Tyr Ile Leu Phe Lys Val Ser Arg Ile Ile Leu Ile Ile Leu His Gln 115 120 12596124PRTHomo sapiensmisc_featureIncyte Clone No 007632 96Met Tyr Lys Leu Ala Ser Cys Cys Leu Leu Phe Ile Gly Phe Leu Asn1 5 10 15Pro Leu Leu Ser Leu Pro Leu Leu Asp Ser Arg Glu Ile Ser Phe Gln 20 25 30Leu Ser Ala Pro His Glu Asp Ala Arg Leu Thr Pro Glu Glu Leu Glu 35 40 45Arg Ala Ser Leu Leu Gln Ile Leu Pro Glu Met Leu Gly Ala Glu Arg 50 55 60Gly Asp Ile Leu Arg Lys Ala Asp Ser Ser Thr Asn Ile Phe Asn Pro65 70 75 80Arg Gly Asn Leu Arg Lys Phe Gln Asp Phe Ser Gly Gln Asp Pro Asn 85 90 95Ile Leu Leu Ser His Leu Leu Ala Arg Ile Trp Lys Pro Tyr Lys Lys 100 105 110Arg Glu Thr Pro Asp Cys Phe Trp Lys Tyr Cys Val 115 12097182PRTHomo sapiensmisc_featureIncyte Clone No 1236968 97Met Trp Pro Leu Ser Ser Asp Ser Ser Trp Ser Leu Trp Ile Ser Thr1 5 10 15Gly Met Ala Pro Ala Pro Ser Ser Ser Thr Arg Ser Phe Ser Glu Ser 20 25 30Leu Lys Gln Lys Leu Val Arg Val Leu Glu Glu Asn Leu Ile Leu Ser 35 40 45Glu Lys Ile Gln Gln Leu Glu Glu Gly Ala Ala Ile Ser Ile Val Ser 50 55 60Gly Gln Gln Ser His Thr Tyr Asp Asp Leu Leu His Lys Asn Gln Gln65 70 75 80Leu Thr Met Gln Val Ala Cys Leu Asn Gln Glu Leu Ala Gln Leu Lys 85 90 95Lys Leu Glu Lys Thr Val Ala Ile Leu His Glu Ser Gln Arg Ser Leu 100 105 110Val Val Thr Asn Glu Tyr Leu Leu Gln Gln Leu Asn Lys Glu Pro Lys 115 120 125Gly Tyr Ser Gly Lys Ala Leu Leu Pro Pro Glu Lys Gly His His Leu 130 135 140Gly Arg Ser Ser Pro Phe Gly Lys Ser Thr Leu Ser Ser Ser Ser Pro145 150 155 160Val Ala His Glu Thr Gly Gln Tyr Leu Ile Gln Ser Val Leu Asp Ala 165 170 175Ala Pro Glu Pro Gly Leu 18098237PRTHomo sapiensmisc_featureIncyte Clone No 1334153 98Met Lys Gly Ile Leu Val Ala Gly Ile Thr Ala Val Leu Val Ala Ala1 5 10 15Val Glu Ser Leu Ser Cys Val Pro Cys Asn Ser Trp Glu Lys Ser Cys 20 25 30Val Asn Ser Ile Ala Ser Glu Cys Pro Ser His Ala Asn Thr Ser Cys 35 40 45Ile Ser Ser Ser Ala Ser Ser Ser Leu Glu Thr Pro Val Arg Leu Tyr 50 55 60Gln Asn Met Phe Cys Ser Ala Glu Asn Cys Ser Glu Glu Thr His Ile65 70 75 80Thr Ala Phe Thr Val His Val Ser Ala Glu Glu His Phe His Phe Val 85 90 95Ser Gln Cys Cys Gln Gly Lys Glu Cys Ser Asn Thr Ser Asp Ala Leu 100 105 110Asp Pro Pro Leu Lys Asn Val Ser Ser Asn Ala Glu Cys Pro Ala Cys 115 120 125Tyr Glu Ser Asn Gly Thr Ser Cys Arg Gly Lys Pro Trp Lys Cys Tyr 130 135 140Glu Glu Glu Gln Cys Val Phe Leu Val Ala Glu Leu Lys Asn Asp Ile145 150 155 160Glu Ser Lys Ser Leu Val Leu Lys Gly Cys Ser Asn Val Ser Asn Ala 165 170 175Thr Cys Gln Phe Leu Ser Gly Glu Asn Lys Thr Leu Gly Gly Val Ile 180 185 190Phe Arg Lys Phe Glu Cys Ala Asn Val Asn Ser Leu Thr Pro Thr Ser 195 200 205Ala Pro Thr Thr Ser His Asn Val Gly Ser Lys Ala Ser Leu Tyr Leu 210 215 220Leu Ala Leu Ala Ser Leu Leu Leu Arg Gly Leu Leu Pro225 230 23599160PRTHomo sapiensmisc_featureIncyte Clone No 1396975 99Met Arg Pro Gly Pro Met Leu Gln Ala Arg Val Ser Ile Pro Ala Ala1 5 10 15Leu Gly Thr Leu Phe Pro Arg Pro Gly Trp Ala Pro Gly Glu Val Ser 20 25 30Ser Glu Ile Ser Ser Arg Asp Leu Leu Asn Pro His Pro Ser Thr Pro 35 40 45Ser Cys Cys Ser Gln Ser Trp Ser Pro Met Ser Val Leu Glu Pro Asp 50 55 60Ser Arg Gly Pro Pro Pro Ile Ser Leu Thr His Thr Gly Ile His Thr65 70 75 80Pro Gln Lys Thr Ser Gln Met Arg Pro Asp Ser Gly Ser Arg Gly Met 85 90 95Cys Phe Cys Pro Cys Lys Gly Phe Gly Glu Gly Gly Asn Ile Val Glu 100 105 110Ala Gly Lys Ser Pro Gln Thr Cys Ala His Ala Pro Pro Ala Leu Arg 115 120 125Phe His Ser Ala Phe Ser Glu Cys Pro Cys Cys Thr Gln Thr Thr Gly 130 135 140Gln Glu Arg Pro Ser Leu Pro Leu Gln Pro Leu Ser Leu Pro Phe Asn145 150 155 160100148PRTHomo sapiensmisc_featureIncyte Clone No 1501749 100Met Ala Ala Ser Pro Ala Arg Pro Ala Val Leu Ala Leu Thr Gly Leu1 5 10 15Ala Leu Leu Leu Leu Leu Cys Trp Gly Pro Gly Gly Ile Ser Gly Asn 20 25 30Lys Leu Lys Leu Met Leu Gln Lys Arg Glu Ala Pro Val Pro Thr Lys 35 40 45Thr Lys Val Ala Val Asp Glu Asn Lys Ala Lys Glu Phe Leu Gly Ser 50 55 60Leu Lys Arg Gln Lys Arg Gln Leu Trp Asp Arg Thr Arg Pro Glu Val65 70 75 80Gln Gln Trp Tyr Gln Gln Phe Leu Tyr Met Gly Phe Asp Glu Ala Lys 85 90 95Phe Glu Asp Asp Ile Thr Tyr Trp Leu Asn Arg Asp Arg Asn Gly His 100 105 110Glu Tyr Tyr Gly Asp Tyr Tyr Gln Arg His Tyr Asp Glu Asp Ser Ala 115 120 125Ile Gly Pro Arg Ser Pro Tyr Gly Phe Arg His Gly Ala Ser Val Asn 130 135 140Tyr Asp Asp Tyr145101170PRTHomo sapiensmisc_featureIncyte Clone No 1575240 101Met Thr Pro Thr Lys Arg Glu Pro Pro Ala Ala Pro Leu Leu Leu Arg1 5 10 15Val Leu Pro Gln Leu Ser Ala Met Ser Leu Arg Leu Ser Thr Arg Arg 20 25 30Glu Asp Met Ile Gly Gln Thr Ser Gly Met Cys Ser Phe Cys Ser Phe 35 40 45Gln Asn Met Arg Gly Glu Ser Ile Trp Leu Leu Cys Leu Glu Glu Glu 50 55 60Gly Ala Gly Leu Cys Gln Asn Ser Leu Asp Lys Arg Phe Ser Gln Lys65 70 75 80Glu Gly Cys Ser Asp Asp Lys Ser Pro Leu His His Phe Pro Trp Leu 85 90 95Ser Asp Ala Pro Pro Ser Ser His Ala Arg Thr Ser Glu Ile Arg Leu 100 105 110Pro Pro Asp Ile Thr Gln Pro Cys Leu Thr Lys Arg Gln Trp Phe Ile 115 120 125Pro Ser Leu Gly Glu Lys Arg Gly Asn Ala Lys Leu Leu His Gln Leu 130 135 140Leu Ile Leu Leu Pro Ala Arg Asn Pro Gly Tyr Leu Gln Val Ser Leu145 150 155 160Pro Leu Val Trp Ser Trp Leu Ser Leu Phe 165 170102150PRTHomo sapiensmisc_featureIncyte Clone No 1647884 102Met Gly Ala Ala Ala Trp Ala Arg Pro Leu Ser Val Ser Phe Leu Leu1 5 10 15Leu Leu Leu Pro Leu Pro Gly Met Pro Ala Gly Ser Trp Asp Pro Ala 20 25 30Gly Tyr Leu Leu Tyr Cys Pro Cys Met Gly Lys Ala Ser Gln Ala Leu 35 40 45Cys Ser Asp Gly Glu Thr Glu Ala Gly Arg Gly Lys Ala Thr Pro Gln 50 55 60Met Arg Pro Glu Thr Pro Ser Gln Val Gln Glu Arg Thr Ser Glu Arg65 70 75 80Asp Gly Ala Cys Ser Ser Pro Leu Cys Leu Ser Cys Lys Gly Thr Glu 85 90 95Gly Pro Thr Cys Pro Thr Phe His Leu Thr Asp Glu Lys Thr Glu Ala 100 105 110Gly Arg Gly Tyr Val Thr Cys Leu Arg Ser Lys Pro Val Gln Gly Pro 115 120 125Val Asn Gly Val Ser Gly Ala Gly Leu Asp Val Thr Asp Pro Arg Trp 130 135 140Leu Leu Val Ile Phe His145 150103142PRTHomo sapiensmisc_featureIncyte Clone No 1661144 103Met Gly Cys Leu Val Trp Gly Pro Ser Trp Pro Pro Leu Ser Leu Leu1 5 10 15Ala Ser Leu Leu His Ser Gly Ile Ala Gly Arg Cys Leu Leu Cys Leu 20 25 30Phe Lys Gly Leu Ala Ala Ala Ala Ser Leu Gln Ile Arg Asp Leu Ala 35 40 45Ser Arg Leu Thr Thr Gly Pro Arg Thr Cys Arg Val Gln Pro Pro Pro 50 55 60His Pro Gln Ser Ser Pro Pro Trp Pro Gly Pro Pro Gly Ala Glu Thr65 70 75 80Cys Arg Pro Leu Ser Arg Thr Val Gly Gly Val Cys Pro Ser Asp Trp

85 90 95Pro Val Ser Trp Leu Leu Leu Pro Pro Leu Pro Glu Val Val Thr Cys 100 105 110Ser Cys Pro Arg Ile Lys Ala Arg Pro Glu Arg Thr Pro Glu Leu Leu 115 120 125Cys Ala Trp Gly Gly Arg Gly Lys His Ser Gln Leu Val Ala 130 135 140104110PRTHomo sapiensmisc_featureIncyte Clone No 1685409 104Met Glu Thr Gly Arg Leu Leu Ser Leu Ser Ser Leu Pro Leu Val Leu1 5 10 15Leu Gly Trp Glu Tyr Ser Ser Gln Thr Leu Asn Leu Val Pro Ser Thr 20 25 30Ser Ile Leu Ser Phe Val Pro Phe Ile Pro Leu His Leu Val Leu Phe 35 40 45Ala Leu Trp Tyr Leu Pro Val Pro His His Leu Tyr Pro Gln Gly Leu 50 55 60Gly Asp His Ala Ala Glu Ala Glu Lys Gly Lys Arg Glu Glu Gly Gly65 70 75 80Thr Gln Val Ala Leu Trp Leu Arg Val Gln Pro Ser Cys Pro Ser Pro 85 90 95Val Cys Leu Glu Pro Val Pro Pro Arg Ser Arg Phe Leu Leu 100 105 110105120PRTHomo sapiensmisc_featureIncyte Clone No 1731419 105Met Ser Arg Ala Gly Met Leu Gly Val Val Cys Ala Leu Leu Val Trp1 5 10 15Ala Tyr Leu Ala Val Gly Lys Leu Val Val Arg Met Thr Phe Thr Glu 20 25 30Leu Cys Thr His His Pro Trp Ser Leu Arg Cys Glu Ser Phe Cys Arg 35 40 45Ser Arg Val Thr Ala Cys Leu Pro Ala Pro Ala Pro Trp Leu Arg Pro 50 55 60Phe Leu Cys Pro Met Leu Phe Ser Asp Arg Asn Pro Val Glu Cys His65 70 75 80Leu Phe Gly Glu Ala Val Ser Asp Pro Val Cys Lys Gly Leu Leu Pro 85 90 95His Tyr Phe Trp His Pro Thr Phe Phe Pro Val Lys Ala Asn Cys Leu 100 105 110Val Ser Phe Cys Pro Thr Thr Val 115 120106135PRTHomo sapiensmisc_featureIncyte Clone No 2650265 106Met Ala Arg Phe Trp Val Cys Val Ala Gly Ala Gly Phe Phe Leu Ala1 5 10 15Phe Leu Val Leu His Ser Arg Phe Cys Gly Ser Pro Val Leu Arg Asn 20 25 30Phe Thr Phe Ala Val Ser Trp Arg Thr Glu Lys Ile Leu Tyr Arg Leu 35 40 45Asp Val Gly Trp Pro Lys His Pro Glu Tyr Phe Thr Gly Thr Thr Phe 50 55 60Cys Val Ala Val Asp Ser Leu Asn Gly Leu Val Tyr Ile Gly Gln Arg65 70 75 80Gly Asp Asn Ile Pro Lys Ile Leu Val Phe Thr Glu Asp Gly Tyr Phe 85 90 95Leu Arg Ala Trp Asn Tyr Thr Val Asp Thr Pro His Gly Ile Phe Ala 100 105 110Ala Ser Thr Leu Tyr Glu Gln Ser Val Trp Ile Thr Asp Val Gly Ser 115 120 125Gly Met Tyr Ser Asn Ile Tyr 130 135107301PRTHomo sapiensmisc_featureIncyte Clone No 2677129 107Met Leu Met Ile Ile Ile Ile Glu Pro Phe Ser Val Leu Ile Leu Phe1 5 10 15Lys Ser Gly Ile Leu Ala Asp Phe Phe Ala Leu Leu Leu Leu Ile Asn 20 25 30Phe Phe Leu Val Ser Phe Phe Leu Ala Tyr Pro Leu Phe Asn Asn Gln 35 40 45Ile Asn Ser Arg Ser Met Asn Glu Ile Lys Asn Leu Gln Tyr Leu Pro 50 55 60Arg Thr Ser Glu Pro Arg Glu Val Leu Phe Glu Asp Arg Thr Arg Ala65 70 75 80His Ala Asp His Val Gly Gln Gly Phe Asp Trp Gln Ser Thr Ala Ala 85 90 95Val Gly Val Leu Lys Ala Val Gln Phe Gly Glu Trp Ser Asp Gln Pro 100 105 110Arg Ile Thr Lys Asp Val Ile Cys Phe His Ala Glu Asp Phe Thr Asp 115 120 125Val Val Gln Arg Leu Gln Leu Asp Leu His Glu Pro Pro Val Ser Gln 130 135 140Cys Val Gln Trp Val Asp Glu Ala Lys Leu Asn Gln Met Arg Arg Glu145 150 155 160Gly Ile Arg Tyr Ala Arg Ile Gln Leu Cys Asp Asn Asp Ile Tyr Phe 165 170 175Ile Pro Arg Asn Val Ile His Gln Phe Lys Thr Val Ser Ala Val Cys 180 185 190Ser Leu Ala Trp His Ile Arg Leu Lys Gln Tyr His Pro Val Val Glu 195 200 205Ala Thr Gln Asn Thr Glu Ser Asn Ser Asn Met Asp Cys Gly Leu Thr 210 215 220Gly Lys Arg Glu Leu Glu Val Asp Ser Gln Cys Val Arg Ile Lys Thr225 230 235 240Glu Ser Glu Glu Ala Cys Thr Glu Ile Gln Leu Leu Thr Thr Ala Ser 245 250 255Ser Ser Phe Pro Pro Ala Ser Glu Leu Asn Leu Gln Gln Asp Gln Lys 260 265 270Thr Gln Pro Ile Pro Val Leu Lys Val Glu Ser Arg Leu Asp Ser Asp 275 280 285Gln Gln His Asn Leu Gln Glu His Ser Thr Thr Ser Val 290 295 300108103PRTHomo sapiensmisc_featureIncyte Clone No 3151073 108Met Ser Phe Val Pro Gly Leu Leu Leu Cys Phe Val Leu Leu Leu Cys1 5 10 15Val Ser Pro Val Tyr Leu Pro Ser Arg Ser Pro Ser Thr Phe Pro Ile 20 25 30Ser Glu Pro Leu Ser Phe Ile Gly Met Ser Ala Trp Pro Gln Cys Ser 35 40 45Pro Ile Tyr Ser Gln Thr Pro Gly Leu Ala Tyr Glu Pro Ser Ser Phe 50 55 60Pro Lys Arg Arg Tyr Trp Val Cys Thr Leu His Glu Ile Lys Trp Glu65 70 75 80Cys Pro Arg Ser Arg Arg Thr Ser Asp Ala Val His Ala Asn Lys Leu 85 90 95Gly Leu Pro Leu Lys Ile Ile 10010995PRTHomo sapiensmisc_featureIncyte Clone No 3170095 109Met Lys Phe Leu Leu Leu Val Leu Ala Ala Leu Gly Phe Leu Thr Gln1 5 10 15Val Ile Pro Ala Ser Ala Gly Gly Ser Lys Cys Val Ser Asn Thr Pro 20 25 30Gly Tyr Cys Arg Thr Cys Cys His Trp Gly Glu Thr Ala Leu Phe Met 35 40 45Cys Asn Ala Ser Arg Lys Cys Cys Ile Ser Tyr Ser Phe Leu Pro Lys 50 55 60Pro Asp Leu Pro Gln Leu Ile Gly Asn His Trp Gln Ser Arg Arg Arg65 70 75 80Asn Thr Gln Arg Lys Asp Lys Lys Gln Gln Thr Thr Val Thr Ser 85 90 95110113PRTHomo sapiensmisc_featureIncyte Clone No 3475168 110Met Ser Pro Ser Pro Arg Trp Gly Phe Leu Cys Val Leu Phe Thr Ala1 5 10 15Val His Pro Ala Pro Ser Thr Ala Pro Val Gln Asp Lys Cys Pro Val 20 25 30Asn Thr Trp Glu Ala Met Gln Ala Ser Ser Gln Gln Leu Leu Gln Thr 35 40 45Asp Pro Arg Pro Lys Pro Phe Leu Leu Pro Pro Leu Pro Pro Leu Leu 50 55 60Leu Ile Ser Ala Gly Thr Glu Val Ser Ser Leu Val Phe Gln Lys Ser65 70 75 80Pro Leu His Thr Gln Pro Glu Gly Ala Ile Lys Thr Ala Gly Gln Pro 85 90 95Thr Ser Val His Ser Lys Val Leu Ser Lys Gly Ser Leu Leu Leu Gly 100 105 110Glu111234PRTHomo sapiensmisc_featureIncyte Clone No 3836893 111Met Arg Lys Thr Arg Leu Trp Gly Leu Leu Trp Met Leu Phe Val Ser1 5 10 15Glu Leu Arg Ala Ala Thr Lys Leu Thr Glu Glu Lys Tyr Glu Leu Lys 20 25 30Glu Gly Gln Thr Leu Asp Val Lys Cys Asp Tyr Thr Leu Glu Lys Phe 35 40 45Ala Ser Ser Gln Lys Ala Trp Gln Ile Ile Arg Asp Gly Glu Met Pro 50 55 60Lys Thr Leu Ala Cys Thr Glu Arg Pro Ser Lys Asn Ser His Pro Val65 70 75 80Gln Val Gly Arg Ile Ile Leu Glu Asp Tyr His Asp His Gly Leu Leu 85 90 95Arg Val Arg Met Val Asn Leu Gln Val Glu Asp Ser Gly Leu Tyr Gln 100 105 110Cys Val Ile Tyr Gln Pro Pro Lys Glu Pro His Met Leu Phe Asp Arg 115 120 125Ile Arg Leu Val Val Thr Lys Gly Phe Ser Gly Thr Pro Gly Ser Asn 130 135 140Glu Asn Ser Thr Gln Asn Val Tyr Lys Ile Pro Pro Thr Thr Thr Lys145 150 155 160Ala Leu Cys Pro Leu Tyr Thr Ser Pro Arg Thr Val Thr Gln Ala Pro 165 170 175Pro Lys Ser Thr Ala Asp Val Ser Thr Pro Asp Ser Glu Ile Asn Leu 180 185 190Thr Asn Val Thr Asp Ile Ile Arg Val Pro Val Phe Asn Ile Val Ile 195 200 205Leu Leu Ala Gly Gly Phe Leu Ser Lys Ser Leu Val Phe Ser Val Leu 210 215 220Phe Ala Val Thr Leu Arg Ser Phe Val Pro225 230112119PRTHomo sapiensmisc_featureIncyte Clone No 4072159 112Met Val Leu Pro Leu Pro Trp Leu Ser Arg Tyr His Phe Leu Arg Leu1 5 10 15Leu Leu Pro Ser Trp Ser Leu Ala Pro Gln Gly Ser His Gly Cys Cys 20 25 30Ser Gln Asn Pro Lys Ala Ser Met Glu Glu Gln Thr Asn Ser Arg Gly 35 40 45Asn Gly Lys Met Thr Ser Pro Pro Arg Gly Pro Gly Thr His Arg Thr 50 55 60Ala Glu Leu Ala Arg Ala Glu Glu Leu Leu Glu Gln Gln Leu Glu Leu65 70 75 80Tyr Gln Ala Leu Leu Glu Gly Gln Glu Gly Ala Trp Glu Ala Gln Ala 85 90 95Leu Val Leu Lys Ile Gln Lys Leu Lys Glu Gln Met Arg Arg His Gln 100 105 110Glu Ser Leu Gly Gly Gly Ala 115113200PRTHomo sapiensmisc_featureIncyte Clone No 1003916 113Met Ala Ser Ser Leu Thr Cys Thr Gly Val Ile Trp Ala Leu Leu Ser1 5 10 15Phe Leu Cys Ala Ala Thr Ser Cys Val Gly Phe Phe Met Pro Tyr Trp 20 25 30Leu Trp Gly Ser Gln Leu Gly Lys Pro Val Ser Phe Gly Thr Phe Arg 35 40 45Arg Cys Ser Tyr Pro Val His Asp Glu Ser Arg Gln Met Met Val Met 50 55 60Val Glu Glu Cys Gly Arg Tyr Ala Ser Phe Gln Gly Ile Pro Ser Ala65 70 75 80Glu Trp Arg Ile Cys Thr Ile Val Thr Gly Leu Gly Cys Gly Leu Leu 85 90 95Leu Leu Val Ala Leu Thr Ala Leu Met Gly Cys Cys Val Ser Asp Leu 100 105 110Ile Ser Arg Thr Val Gly Arg Val Ala Gly Gly Ile Gln Phe Leu Gly 115 120 125Gly Leu Leu Ile Gly Ala Gly Cys Ala Leu Tyr Pro Leu Gly Trp Asp 130 135 140Ser Glu Glu Val Arg Gln Thr Cys Gly Tyr Thr Ser Gly Gln Phe Asp145 150 155 160Leu Gly Lys Cys Glu Ile Gly Trp Ala Tyr Tyr Cys Thr Gly Ala Gly 165 170 175Ala Thr Ala Ala Met Leu Leu Cys Thr Trp Leu Ala Cys Phe Ser Gly 180 185 190Lys Lys Gln Lys His Tyr Pro Tyr 195 200114225PRTHomo sapiensmisc_featureIncyte Clone No 2093492 114Met Gly Phe Arg Leu Glu Gly Ile Phe Pro Ala Ala Leu Leu Pro Leu1 5 10 15Leu Leu Thr Met Ile Leu Phe Leu Gly Pro Leu Met Gln Leu Ser Met 20 25 30Asp Cys Pro Cys Asp Leu Ala Asp Gly Leu Lys Val Val Leu Ala Pro 35 40 45Arg Ser Trp Ala Arg Cys Leu Thr Asp Met Arg Trp Leu Arg Asn Gln 50 55 60Val Ile Ala Pro Leu Thr Glu Glu Leu Val Phe Arg Ala Cys Met Leu65 70 75 80Pro Met Leu Ala Pro Cys Met Gly Leu Gly Pro Ala Val Phe Thr Cys 85 90 95Pro Leu Phe Phe Gly Val Ala His Phe His His Ile Ile Glu Gln Leu 100 105 110Arg Phe Arg Gln Ser Ser Val Gly Asn Ile Phe Leu Ser Ala Ala Phe 115 120 125Gln Phe Ser Tyr Thr Ala Val Phe Gly Ala Tyr Thr Ala Phe Leu Phe 130 135 140Ile Arg Thr Gly His Leu Ile Gly Pro Val Leu Cys His Ser Phe Cys145 150 155 160Asn Tyr Met Gly Phe Pro Ala Val Cys Ala Ala Leu Glu His Pro Gln 165 170 175Arg Arg Pro Leu Leu Ala Gly Tyr Ala Leu Gly Val Gly Leu Phe Leu 180 185 190Leu Leu Leu Gln Pro Leu Thr Asp Pro Lys Leu Tyr Gly Ser Leu Pro 195 200 205Leu Cys Val Leu Leu Glu Arg Ala Gly Asp Ser Glu Ala Pro Leu Cys 210 215 220Ser225115155PRTHomo sapiensmisc_featureIncyte Clone No 2108789 115Met Ser Gly Leu Leu Ile Pro Pro Leu Pro Gly Trp Val Leu Gly Pro1 5 10 15Leu Met Trp Ala Cys Arg Pro Pro Gln Asp Glu Pro Ser Gly Thr Asp 20 25 30Pro Pro Pro Pro Arg Leu Gln Pro His His Val Ser Gly Leu Gly Leu 35 40 45Gly Gln Ala Trp Ala Gln Ser Trp Ala Pro Arg Gly Ser Pro Pro Leu 50 55 60Thr Trp Leu Leu Pro Thr Leu Pro Leu Lys Asp Gly Pro Ala Ala Arg65 70 75 80Leu Pro Pro Pro Pro His Thr Thr Leu Gly Gly Leu Ser His Pro Pro 85 90 95Gln Pro Arg Ser Ala Gln Thr Asp Pro His Ser Ile Pro Arg Pro Ala 100 105 110Ala Gln Val Arg Gly Pro Val Leu Pro Gly Ala Trp Ala Thr Pro Tyr 115 120 125Ala Ile Ser Ser Glu Gln Pro Gly Pro Thr Asp Pro His Ala Leu Ser 130 135 140Tyr Val Pro Phe Ser Pro Asp Phe Phe Cys Thr145 150 155116468PRTHomo sapiensmisc_featureIncyte Clone No 2171401 116Met Gly Arg Gly Trp Gly Phe Leu Phe Gly Leu Leu Gly Ala Val Trp1 5 10 15Leu Leu Ser Ser Gly His Gly Glu Glu Gln Pro Pro Glu Thr Ala Ala 20 25 30Gln Arg Cys Phe Cys Gln Val Ser Gly Tyr Leu Asp Asp Cys Thr Cys 35 40 45Asp Val Glu Thr Ile Asp Arg Phe Asn Asn Tyr Arg Leu Phe Pro Arg 50 55 60Leu Gln Lys Leu Leu Glu Ser Asp Tyr Phe Arg Tyr Tyr Lys Val Asn65 70 75 80Leu Lys Arg Pro Cys Pro Phe Trp Asn Asp Ile Ser Gln Cys Gly Arg 85 90 95Arg Asp Cys Ala Val Lys Pro Cys Gln Ser Asp Glu Val Pro Asp Gly 100 105 110Ile Lys Ser Ala Ser Tyr Lys Tyr Ser Glu Glu Ala Asn Asn Leu Ile 115 120 125Glu Glu Cys Glu Gln Ala Glu Arg Leu Gly Ala Val Asp Glu Ser Leu 130 135 140Ser Glu Glu Thr Gln Lys Ala Val Leu Gln Trp Thr Lys His Asp Asp145 150 155 160Ser Ser Asp Asn Phe Cys Glu Ala Asp Asp Ile Gln Ser Pro Glu Ala 165 170 175Glu Tyr Val Asp Leu Leu Leu Asn Pro Glu Arg Tyr Thr Gly Tyr Lys 180 185 190Gly Pro Asp Ala Trp Lys Ile Trp Asn Val Ile Tyr Glu Glu Asn Cys 195 200 205Phe Lys Pro Gln Thr Ile Lys Arg Pro Leu Asn Pro Leu Ala Ser Gly 210 215 220Gln Gly Thr Ser Glu Glu Asn Thr Phe Tyr Ser Trp Leu Glu Gly Leu225 230 235 240Cys Val Glu Lys Arg Ala Phe Tyr Arg Leu Ile Ser Gly Leu His Ala 245 250 255Ser Ile Asn Val His Leu Ser Ala Arg Tyr Leu Leu Gln Glu Thr Trp 260 265 270Leu Glu Lys Lys Trp Gly His Asn Ile Thr Glu Phe Gln Gln Arg Phe 275 280 285Asp Gly Ile Leu Thr Glu Gly Glu Gly Pro Arg Arg Leu Lys Asn Leu 290 295 300Tyr Phe Leu Tyr Leu Ile Glu Leu Arg Ala Leu Ser Lys Val Leu Pro305 310 315 320Phe Phe Glu Arg Pro Asp Phe Gln Leu Phe Thr Gly Asn Lys Ile Gln 325 330 335Asp Glu Glu Asn Lys Met Leu Leu Leu Glu Ile Leu His Glu Ile Lys 340 345 350Ser Phe Pro Leu His Phe Asp Glu Asn Ser Phe Phe Ala Gly Asp Lys 355 360 365Lys Glu Ala His Lys Leu Lys Glu Asp Phe Arg Leu His Phe Arg Asn 370 375 380Ile Ser Arg Ile Met Asp Cys Val Gly Cys Phe Lys Cys Arg Leu Trp385 390 395 400Gly Lys Leu Gln Thr Gln Gly Leu Gly Thr Ala Leu Lys Ile Leu Phe 405 410

415Ser Glu Lys Leu Ile Ala Asn Met Pro Glu Ser Gly Pro Ser Tyr Glu 420 425 430Phe His Leu Thr Arg Gln Glu Ile Val Ser Leu Phe Asn Ala Phe Gly 435 440 445Arg Ile Ser Thr Ser Val Lys Glu Leu Glu Asn Phe Arg Asn Leu Leu 450 455 460Gln Asn Ile His465117403PRTHomo sapiensmisc_featureIncyte Clone No 2212530 117Met Ser Thr Ser Thr Ser Pro Ala Ala Met Leu Leu Arg Arg Leu Arg1 5 10 15Arg Leu Ser Trp Gly Ser Thr Ala Val Gln Leu Phe Ile Leu Thr Val 20 25 30Val Thr Phe Gly Leu Leu Ala Pro Leu Ala Cys His Arg Leu Leu His 35 40 45Ser Tyr Phe Tyr Leu Arg His Trp His Leu Asn Gln Met Ser Gln Glu 50 55 60Phe Leu Gln Gln Ser Leu Lys Glu Gly Glu Ala Ala Leu His Tyr Phe65 70 75 80Glu Glu Leu Pro Ser Ala Asn Gly Ser Val Pro Ile Val Trp Gln Ala 85 90 95Thr Pro Arg Pro Trp Leu Val Ile Thr Ile Ile Thr Val Asp Arg Gln 100 105 110Pro Gly Phe His Tyr Val Leu Gln Val Val Ser Gln Phe His Arg Leu 115 120 125Leu Gln Gln Cys Gly Pro Gln Cys Glu Gly His Gln Leu Phe Leu Cys 130 135 140Asn Val Glu Arg Ser Val Ser His Phe Asp Ala Lys Leu Leu Ser Lys145 150 155 160Tyr Val Pro Val Ala Asn Arg Tyr Glu Gly Thr Glu Asp Asp Tyr Gly 165 170 175Asp Asp Pro Ser Thr Asn Ser Phe Glu Lys Glu Lys Gln Asp Tyr Val 180 185 190Tyr Cys Leu Glu Ser Ser Leu Gln Thr Tyr Asn Pro Asp Tyr Val Leu 195 200 205Met Val Glu Asp Asp Ala Val Pro Glu Glu Gln Ile Phe Pro Val Leu 210 215 220Glu His Leu Leu Arg Ala Arg Phe Ser Glu Pro His Leu Arg Asp Ala225 230 235 240Leu Tyr Leu Lys Leu Tyr His Pro Glu Arg Leu Gln His Tyr Ile Asn 245 250 255Pro Glu Pro Met Arg Ile Leu Glu Trp Val Gly Val Gly Met Leu Leu 260 265 270Gly Pro Leu Leu Thr Trp Ile Tyr Met Arg Phe Ala Ser Arg Pro Gly 275 280 285Phe Ser Trp Pro Val Met Leu Phe Phe Ser Leu Tyr Ser Met Gly Leu 290 295 300Val Glu Leu Val Gly Arg His Tyr Phe Leu Glu Leu Arg Arg Leu Ser305 310 315 320Pro Ser Leu Tyr Ser Val Val Pro Ala Ser Gln Cys Cys Thr Pro Ala 325 330 335Met Leu Phe Pro Ala Pro Ala Ala Arg Arg Thr Leu Thr Tyr Leu Ser 340 345 350Gln Val Tyr Cys His Lys Gly Phe Gly Lys Asp Met Ala Leu Tyr Ser 355 360 365Leu Leu Arg Ala Lys Gly Glu Arg Ala Tyr Val Val Glu Pro Asn Leu 370 375 380Val Lys His Ile Gly Leu Phe Ser Ser Leu Arg Tyr Asn Phe His Pro385 390 395 400Ser Leu Leu118131PRTHomo sapiensmisc_featureIncyte Clone No 2253036 118Met Glu Arg Cys Phe His Cys Phe Pro Val His Leu Val Phe Asn Leu1 5 10 15Val Gln Ser Phe Ser Pro Ile Ser Gly Val Glu Ser Cys Leu Leu Pro 20 25 30Gln Cys Asp Lys Cys Trp Pro Met Val Tyr Arg Ser Cys Asp Ala Ser 35 40 45Arg Gly Leu Val Asn Ala Cys Ile Leu Gly Phe Val Leu Leu Glu Cys 50 55 60Ser Phe Val Gly Ala Leu Asn Asn Tyr Val Arg Ser Leu Ala Thr Leu65 70 75 80Leu Glu Arg Thr His Gly Gly Lys Arg Leu Lys Leu Cys Glu Glu Ser 85 90 95Gln Ala Ser His Pro Ser Phe Ser Ala Glu Pro Arg His Gln Pro Thr 100 105 110Cys Gln Leu Asn Ala Thr Val Arg Val Ile Thr Ser Lys Ile Thr Arg 115 120 125Lys Thr Thr 130119556PRTHomo sapiensmisc_featureIncyte Clone No 2280161 119Met Ala Ala Ala Ala Trp Leu Gln Val Leu Pro Val Ile Leu Leu Leu1 5 10 15Leu Gly Ala His Pro Ser Pro Leu Ser Phe Phe Ser Ala Gly Pro Ala 20 25 30Thr Val Ala Ala Ala Asp Arg Ser Lys Trp His Ile Pro Ile Pro Ser 35 40 45Gly Lys Asn Tyr Phe Ser Phe Gly Lys Ile Leu Phe Arg Asn Thr Thr 50 55 60Ile Phe Leu Lys Phe Asp Gly Glu Pro Cys Asp Leu Ser Leu Asn Ile65 70 75 80Thr Trp Tyr Leu Lys Ser Ala Asp Cys Tyr Asn Glu Ile Tyr Asn Phe 85 90 95Lys Ala Glu Glu Val Glu Leu Tyr Leu Glu Lys Leu Lys Glu Lys Arg 100 105 110Gly Leu Ser Gly Lys Tyr Gln Thr Ser Ser Lys Leu Phe Gln Asn Cys 115 120 125Ser Glu Leu Phe Lys Thr Gln Thr Phe Ser Gly Asp Phe Met His Arg 130 135 140Leu Pro Leu Leu Gly Glu Lys Gln Glu Ala Lys Glu Asn Gly Thr Asn145 150 155 160Leu Thr Phe Ile Gly Asp Lys Thr Ala Met His Glu Pro Leu Gln Thr 165 170 175Trp Gln Asp Ala Pro Tyr Ile Phe Ile Val His Ile Gly Ile Ser Ser 180 185 190Ser Lys Glu Ser Ser Lys Glu Asn Ser Leu Ser Asn Leu Phe Thr Met 195 200 205Thr Val Glu Val Lys Gly Pro Tyr Glu Tyr Leu Thr Leu Glu Asp Tyr 210 215 220Pro Leu Met Ile Phe Phe Met Val Met Cys Ile Val Tyr Val Leu Phe225 230 235 240Gly Val Leu Trp Leu Ala Trp Ser Ala Cys Tyr Trp Arg Asp Leu Leu 245 250 255Arg Ile Gln Phe Trp Ile Gly Ala Val Ile Phe Leu Gly Met Leu Glu 260 265 270Lys Ala Val Phe Tyr Ala Glu Phe Gln Asn Ile Arg Tyr Lys Gly Glu 275 280 285Ser Val Gln Gly Ala Leu Ile Leu Ala Glu Leu Leu Ser Ala Val Lys 290 295 300Arg Ser Leu Ala Arg Thr Leu Val Ile Ile Val Ser Leu Gly Tyr Gly305 310 315 320Ile Val Lys Pro Arg Leu Gly Val Thr Leu His Lys Val Val Val Ala 325 330 335Gly Ala Leu Tyr Leu Leu Phe Ser Gly Met Glu Gly Val Leu Arg Val 340 345 350Thr Gly Tyr Phe Ser Tyr Pro Leu Thr Leu Ile Val Asn Leu Ala Leu 355 360 365Ser Ala Val Asp Ala Cys Val Ile Leu Trp Ile Phe Ile Ser Leu Thr 370 375 380Gln Thr Met Lys Leu Leu Lys Leu Arg Arg Asn Ile Val Lys Leu Ser385 390 395 400Leu Tyr Arg His Phe Thr Asn Thr Leu Ile Leu Ala Val Ala Ala Ser 405 410 415Ile Val Phe Ile Ile Trp Thr Thr Met Lys Phe Arg Ile Val Thr Cys 420 425 430Gln Ser Asp Trp Arg Glu Leu Trp Val Asp Asp Ala Ile Trp Arg Leu 435 440 445Leu Phe Ser Met Ile Leu Phe Val Ile Met Val Leu Trp Arg Pro Ser 450 455 460Ala Asn Asn Gln Arg Phe Ala Phe Ser Pro Leu Ser Glu Glu Glu Glu465 470 475 480Glu Asp Glu Gln Lys Glu Pro Met Leu Lys Glu Ser Phe Glu Gly Met 485 490 495Lys Met Arg Ser Thr Lys Gln Glu Pro Asn Gly Asn Ser Lys Val Asn 500 505 510Lys Ala Gln Glu Asp Asp Leu Lys Trp Val Glu Glu Asn Val Pro Ser 515 520 525Ser Val Thr Asp Val Ala Leu Pro Ala Leu Leu Asp Ser Asp Glu Glu 530 535 540Arg Met Ile Thr His Phe Glu Arg Ser Lys Met Glu545 550 555120514PRTHomo sapiensmisc_featureIncyte Clone No 2287485 120Met Ser Trp Pro Arg Arg Leu Leu Leu Arg Tyr Leu Phe Pro Ala Leu1 5 10 15Leu Leu His Gly Leu Gly Glu Gly Ser Ala Leu Leu His Pro Asp Ser 20 25 30Arg Ser His Pro Arg Ser Leu Glu Lys Ser Ala Trp Arg Ala Phe Lys 35 40 45Glu Ser Gln Cys His His Met Leu Lys His Leu His Asn Gly Ala Arg 50 55 60Ile Thr Val Gln Met Pro Pro Thr Ile Glu Gly His Trp Val Ser Thr65 70 75 80Gly Cys Glu Val Arg Ser Gly Pro Glu Phe Ile Thr Arg Ser Tyr Arg 85 90 95Phe Tyr His Asn Asn Thr Phe Lys Ala Tyr Gln Phe Tyr Tyr Gly Ser 100 105 110Asn Arg Cys Thr Asn Pro Thr Tyr Thr Leu Ile Ile Arg Gly Lys Ile 115 120 125Arg Leu Arg Gln Ala Ser Trp Ile Ile Arg Gly Gly Thr Glu Ala Asp 130 135 140Tyr Gln Leu His Asn Val Gln Val Ile Cys His Thr Glu Ala Val Ala145 150 155 160Glu Lys Leu Gly Gln Gln Val Asn Arg Thr Cys Pro Gly Phe Leu Ala 165 170 175Asp Gly Gly Pro Trp Val Gln Asp Val Ala Tyr Asp Leu Trp Arg Glu 180 185 190Glu Asn Gly Cys Glu Cys Thr Lys Ala Val Asn Phe Ala Met His Glu 195 200 205Leu Gln Leu Ile Arg Val Glu Lys Gln Tyr Leu His His Asn Leu Asp 210 215 220His Leu Val Glu Glu Leu Phe Leu Gly Asp Ile His Thr Asp Ala Thr225 230 235 240Gln Arg Met Phe Tyr Arg Pro Ser Ser Tyr Gln Pro Pro Leu Gln Asn 245 250 255Ala Lys Asn His Asp His Ala Cys Ile Ala Cys Arg Ile Ile Tyr Arg 260 265 270Ser Asp Glu His His Pro Pro Ile Leu Pro Pro Lys Ala Asp Leu Thr 275 280 285Ile Gly Leu His Gly Glu Trp Val Ser Gln Arg Cys Glu Val Arg Pro 290 295 300Glu Val Leu Phe Leu Thr Arg His Phe Ile Phe His Asp Asn Asn Asn305 310 315 320Thr Trp Glu Gly His Tyr Tyr His Tyr Ser Asp Pro Val Cys Lys His 325 330 335Pro Thr Phe Ser Ile Tyr Ala Arg Gly Arg Tyr Ser Arg Gly Val Leu 340 345 350Ser Ser Arg Val Met Gly Gly Thr Glu Phe Val Phe Lys Val Asn His 355 360 365Met Lys Val Thr Pro Met Asp Ala Ala Thr Ala Ser Leu Leu Asn Val 370 375 380Phe Asn Gly Asn Glu Cys Gly Ala Glu Gly Ser Trp Gln Val Gly Ile385 390 395 400Gln Gln Asp Val Thr His Thr Asn Gly Cys Val Ala Leu Gly Ile Lys 405 410 415Leu Pro His Thr Glu Tyr Glu Ile Phe Lys Met Glu Gln Asp Ala Arg 420 425 430Gly Arg Tyr Leu Leu Phe Asn Gly Gln Arg Pro Ser Asp Gly Ser Ser 435 440 445Pro Asp Arg Pro Glu Lys Arg Ala Thr Ser Tyr Gln Met Pro Leu Val 450 455 460Gln Cys Ala Ser Ser Ser Pro Arg Ala Glu Asp Leu Ala Glu Asp Ser465 470 475 480Gly Ser Ser Leu Tyr Gly Arg Ala Pro Gly Arg His Thr Trp Ser Leu 485 490 495Leu Leu Ala Ala Leu Ala Cys Leu Val Pro Leu Leu His Trp Asn Ile 500 505 510Arg Arg121109PRTHomo sapiensmisc_featureIncyte Clone No 2380344 121Met Leu Trp Trp Leu Val Leu Leu Leu Leu Pro Thr Leu Lys Ser Val1 5 10 15Phe Cys Ser Leu Val Thr Ser Leu Tyr Leu Pro Asn Thr Glu Asp Leu 20 25 30Ser Leu Trp Leu Trp Pro Lys Pro Asp Leu His Ser Gly Thr Arg Thr 35 40 45Glu Val Ser Thr His Thr Val Pro Ser Lys Pro Gly Thr Ala Ser Pro 50 55 60Cys Trp Pro Leu Ala Gly Ala Val Pro Ser Pro Thr Val Ser Arg Leu65 70 75 80Glu Ala Leu Thr Arg Ala Val Gln Val Ala Glu Pro Leu Gly Ser Cys 85 90 95Gly Phe Gln Gly Gly Pro Cys Pro Gly Arg Arg Arg Asp 100 105122431PRTHomo sapiensmisc_featureIncyte Clone No 2383171 122Met Ser Trp Val Gln Ala Thr Leu Leu Ala Arg Gly Leu Cys Arg Ala1 5 10 15Trp Gly Gly Thr Cys Gly Ala Ala Leu Thr Gly Thr Ser Ile Ser Gln 20 25 30Val Pro Arg Arg Leu Pro Arg Gly Leu His Cys Ser Ala Ala Ala His 35 40 45Ser Ser Glu Gln Ser Leu Val Pro Ser Pro Pro Glu Pro Arg Gln Arg 50 55 60Pro Thr Lys Ala Leu Val Pro Phe Glu Asp Leu Phe Gly Gln Ala Pro65 70 75 80Gly Gly Glu Arg Asp Lys Ala Ser Phe Leu Gln Thr Val Gln Lys Phe 85 90 95Ala Glu His Ser Val Arg Lys Arg Gly His Ile Asp Phe Ile Tyr Leu 100 105 110Ala Leu Arg Lys Met Arg Glu Tyr Gly Val Glu Arg Asp Leu Ala Val 115 120 125Tyr Asn Gln Leu Leu Asn Ile Phe Pro Lys Glu Val Phe Arg Pro Arg 130 135 140Asn Ile Ile Gln Arg Ile Phe Val His Tyr Pro Arg Gln Gln Glu Cys145 150 155 160Gly Ile Ala Val Leu Glu Gln Met Glu Asn His Gly Val Met Pro Asn 165 170 175Lys Glu Thr Glu Phe Leu Leu Ile Gln Ile Phe Gly Arg Lys Ser Tyr 180 185 190Pro Met Leu Lys Leu Val Arg Leu Lys Leu Trp Phe Pro Arg Phe Met 195 200 205Asn Val Asn Pro Phe Pro Val Pro Arg Asp Leu Pro Gln Asp Pro Val 210 215 220Glu Leu Ala Met Phe Gly Leu Arg His Met Glu Pro Asp Leu Ser Ala225 230 235 240Arg Val Thr Ile Tyr Gln Val Pro Leu Pro Lys Asp Ser Thr Gly Ala 245 250 255Ala Asp Pro Pro Gln Pro His Ile Val Gly Ile Gln Ser Pro Asp Gln 260 265 270Gln Ala Ala Leu Ala Arg His Asn Pro Ala Arg Pro Val Phe Val Glu 275 280 285Gly Pro Phe Ser Leu Trp Leu Arg Asn Lys Cys Val Tyr Tyr His Ile 290 295 300Leu Arg Ala Asp Leu Leu Pro Pro Glu Glu Arg Glu Val Glu Glu Thr305 310 315 320Pro Glu Glu Trp Asn Leu Tyr Tyr Pro Met Gln Leu Asp Leu Glu Tyr 325 330 335Val Arg Ser Gly Trp Asp Asn Tyr Glu Phe Asp Ile Asn Glu Val Glu 340 345 350Glu Gly Pro Val Phe Ala Met Cys Met Ala Gly Ala His Asp Gln Ala 355 360 365Thr Met Ala Lys Trp Ile Gln Gly Leu Gln Glu Thr Asn Pro Thr Leu 370 375 380Ala Gln Ile Pro Val Val Phe Arg Leu Ala Gly Ser Thr Arg Glu Leu385 390 395 400Gln Thr Ser Ser Ala Gly Leu Glu Glu Pro Pro Leu Pro Glu Asp His 405 410 415Gln Glu Glu Asp Asp Asn Leu Gln Arg Gln Gln Gln Gly Gln Ser 420 425 430123142PRTHomo sapiensmisc_featureIncyte Clone No 2396046 123Met Leu Leu Gly Val Arg Ala Val Pro Leu Cys Ser Ala Trp Gln Gly1 5 10 15Ala Val Gly Leu Val Ser Leu Ala Ile Ser Ile Cys Lys His Gly Leu 20 25 30Ser Ser Gln Gln Asn Leu Val Pro Gly Lys Ser Asn Val Pro Lys Ala 35 40 45Ser Asp Met Pro Arg Cys Pro Pro Val Phe Gln Ser Pro Asn Leu Thr 50 55 60Pro Phe Pro His His Thr Lys His Thr Ser Gln Gly Ser His Leu Gly65 70 75 80Val Pro Pro Pro Ala Pro Met Pro Trp Cys Pro Gln Ala Gln Gly Phe 85 90 95Gly Leu Ser Cys Gln Ser Leu Asp Ala Phe Glu Gly Gln Leu Gly Cys 100 105 110Gly Trp Gly Val Gln Ala Ala Gly Glu Pro Arg Leu Arg Ile Ile His 115 120 125Thr Leu Leu Phe Gly Ala Phe Val Glu Val Ser Arg Ile Pro 130 135 140124643PRTHomo sapiensmisc_featureIncyte Clone No 2456587 124Met Glu Cys Cys Arg Arg Ala Thr Pro Gly Thr Leu Leu Leu Phe Leu1 5 10 15Ala Phe Leu Leu Leu Ser Ser Arg Thr Ala Arg Ser Glu Glu Asp Arg 20 25 30Asp Gly Leu Trp Asp Ala Trp Gly Pro Trp Ser Glu Cys Ser Arg Thr 35 40 45Cys Gly Gly Gly Ala Ser Tyr Ser Leu Arg Arg Cys Leu Ser Ser Lys 50 55 60Ser Cys Glu Gly Arg Asn Ile Arg Tyr Arg Thr Cys Ser Asn Val Asp65 70 75

80Cys Pro Pro Glu Ala Gly Asp Phe Arg Ala Gln Gln Cys Ser Ala His 85 90 95Asn Asp Val Lys His His Gly Gln Phe Tyr Glu Trp Leu Pro Val Ser 100 105 110Asn Asp Pro Asp Asn Pro Cys Ser Leu Lys Cys Gln Ala Lys Gly Thr 115 120 125Thr Leu Val Val Glu Leu Ala Pro Lys Val Leu Asp Gly Thr Arg Cys 130 135 140Tyr Thr Glu Ser Leu Asp Met Cys Ile Ser Gly Leu Cys Gln Ile Val145 150 155 160Gly Cys Asp His Gln Leu Gly Ser Thr Val Lys Glu Asp Asn Cys Gly 165 170 175Val Cys Asn Gly Asp Gly Ser Thr Cys Arg Leu Val Arg Gly Gln Tyr 180 185 190Lys Ser Gln Leu Ser Ala Thr Lys Ser Asp Asp Thr Val Val Ala Ile 195 200 205Pro Tyr Gly Ser Arg His Ile Arg Leu Val Leu Lys Gly Pro Asp His 210 215 220Leu Tyr Leu Glu Thr Lys Thr Leu Gln Gly Thr Lys Gly Glu Asn Ser225 230 235 240Leu Ser Ser Thr Gly Thr Phe Leu Val Asp Asn Ser Ser Val Asp Phe 245 250 255Gln Lys Phe Pro Asp Lys Glu Ile Leu Arg Met Ala Gly Pro Leu Thr 260 265 270Ala Asp Phe Ile Val Lys Ile Arg Asn Ser Gly Ser Ala Asp Ser Thr 275 280 285Val Gln Phe Ile Phe Tyr Gln Pro Ile Ile His Arg Trp Arg Glu Thr 290 295 300Asp Phe Phe Pro Cys Ser Ala Thr Cys Gly Gly Gly Tyr Gln Leu Thr305 310 315 320Ser Ala Glu Cys Tyr Asp Leu Arg Ser Asn Arg Val Val Ala Asp Gln 325 330 335Tyr Cys His Tyr Tyr Pro Glu Asn Ile Lys Pro Lys Pro Lys Leu Gln 340 345 350Glu Cys Asn Leu Asp Pro Cys Pro Ala Ser Asp Gly Tyr Lys Gln Ile 355 360 365Met Pro Tyr Asp Leu Tyr His Pro Leu Pro Arg Trp Glu Ala Thr Pro 370 375 380Trp Thr Ala Cys Ser Ser Ser Cys Gly Gly Gly Ile Gln Ser Arg Ala385 390 395 400Val Ser Cys Val Glu Glu Asp Ile Gln Gly His Val Thr Ser Val Glu 405 410 415Glu Trp Lys Cys Met Tyr Thr Pro Lys Met Pro Ile Ala Gln Pro Cys 420 425 430Asn Ile Phe Asp Cys Pro Lys Trp Leu Ala Gln Glu Trp Ser Pro Cys 435 440 445Thr Val Thr Cys Gly Gln Gly Leu Arg Tyr Arg Val Val Leu Cys Ile 450 455 460Asp His Arg Gly Met His Thr Gly Gly Cys Ser Pro Lys Thr Lys Pro465 470 475 480His Ile Lys Glu Glu Cys Ile Val Pro Thr Pro Cys Tyr Lys Pro Lys 485 490 495Glu Lys Leu Pro Val Glu Ala Lys Leu Pro Trp Phe Lys Gln Ala Gln 500 505 510Glu Leu Glu Glu Gly Ala Ala Val Ser Glu Glu Pro Ser Phe Ile Pro 515 520 525Glu Ala Trp Ser Ala Cys Thr Val Thr Cys Gly Val Gly Thr Gln Val 530 535 540Arg Ile Val Arg Cys Gln Val Leu Leu Ser Phe Ser Gln Ser Val Ala545 550 555 560Asp Leu Pro Ile Asp Glu Cys Glu Gly Pro Lys Pro Ala Ser Gln Arg 565 570 575Ala Cys Tyr Ala Gly Pro Cys Ser Gly Glu Ile Pro Glu Phe Asn Pro 580 585 590Asp Glu Thr Asp Gly Leu Phe Gly Gly Leu Gln Asp Phe Asp Glu Leu 595 600 605Tyr Asp Trp Glu Tyr Glu Gly Phe Thr Lys Cys Ser Glu Ser Cys Gly 610 615 620Gly Gly Val Gln Glu Ala Val Val Ser Cys Leu Asn Lys Gln Thr Arg625 630 635 640Glu Pro Cys125568PRTHomo sapiensmisc_featureIncyte Clone No 2484813 125Met Val Leu Leu His Trp Cys Leu Leu Trp Leu Leu Phe Pro Leu Ser1 5 10 15Ser Arg Thr Gln Lys Leu Pro Thr Arg Asp Glu Glu Leu Phe Gln Met 20 25 30Gln Ile Arg Asp Lys Ala Phe Phe His Asp Ser Ser Val Ile Pro Asp 35 40 45Gly Ala Glu Ile Ser Ser Tyr Leu Phe Arg Asp Thr Pro Lys Arg Tyr 50 55 60Phe Phe Val Val Glu Glu Asp Asn Thr Pro Leu Ser Val Thr Val Thr65 70 75 80Pro Cys Asp Ala Pro Leu Glu Trp Lys Leu Ser Leu Gln Glu Leu Pro 85 90 95Glu Asp Arg Ser Gly Glu Gly Ser Gly Asp Leu Glu Pro Leu Glu Gln 100 105 110Gln Lys Gln Gln Ile Ile Asn Glu Glu Gly Thr Glu Leu Phe Ser Tyr 115 120 125Lys Gly Asn Asp Val Glu Tyr Phe Ile Ser Ser Ser Ser Pro Ser Gly 130 135 140Leu Tyr Gln Leu Asp Leu Leu Ser Thr Glu Lys Asp Thr His Phe Lys145 150 155 160Val Tyr Ala Thr Thr Thr Pro Glu Ser Asp Gln Pro Tyr Pro Glu Leu 165 170 175Pro Tyr Asp Pro Arg Val Asp Val Thr Ser Leu Gly Arg Thr Thr Val 180 185 190Thr Leu Ala Trp Lys Pro Ser Pro Thr Ala Ser Leu Leu Lys Gln Pro 195 200 205Ile Gln Tyr Cys Val Val Ile Asn Lys Glu His Asn Phe Lys Ser Leu 210 215 220Cys Ala Val Glu Ala Lys Leu Ser Ala Asp Asp Ala Phe Met Met Ala225 230 235 240Pro Lys Pro Gly Leu Asp Phe Ser Pro Phe Asp Phe Ala His Phe Gly 245 250 255Phe Pro Ser Asp Asn Ser Gly Lys Glu Arg Ser Phe Gln Ala Lys Pro 260 265 270Ser Pro Lys Leu Gly Arg His Val Tyr Ser Arg Pro Lys Val Asp Ile 275 280 285Gln Lys Ile Cys Ile Gly Asn Lys Asn Ile Phe Thr Val Ser Asp Leu 290 295 300Lys Pro Asp Thr Gln Tyr Tyr Phe Asp Val Phe Val Val Asn Ile Asn305 310 315 320Ser Asn Met Ser Thr Ala Tyr Val Gly Thr Phe Ala Arg Thr Lys Glu 325 330 335Glu Ala Lys Gln Lys Thr Val Glu Leu Lys Asp Gly Lys Ile Thr Asp 340 345 350Val Phe Val Lys Arg Lys Gly Ala Lys Phe Leu Arg Phe Ala Pro Val 355 360 365Ser Ser His Gln Lys Val Thr Phe Phe Ile His Ser Cys Leu Asp Ala 370 375 380Val Gln Ile Gln Val Arg Arg Asp Gly Lys Leu Leu Leu Ser Gln Asn385 390 395 400Val Glu Gly Ile Gln Gln Phe Gln Leu Arg Gly Lys Pro Lys Ala Lys 405 410 415Tyr Leu Val Arg Leu Lys Gly Asn Lys Lys Gly Ala Ser Met Leu Lys 420 425 430Ile Leu Ala Thr Thr Arg Pro Thr Lys Gln Ser Phe Pro Ser Leu Pro 435 440 445Glu Asp Thr Arg Ile Lys Ala Phe Asp Lys Leu Arg Thr Cys Ser Ser 450 455 460Ala Thr Val Ala Trp Leu Gly Thr Gln Glu Arg Asn Lys Phe Cys Ile465 470 475 480Tyr Lys Lys Glu Val Asp Asp Asn Tyr Asn Glu Asp Gln Lys Lys Arg 485 490 495Glu Gln Asn Gln Cys Leu Gly Pro Asp Ile Arg Lys Lys Ser Glu Lys 500 505 510Val Leu Cys Lys Tyr Phe His Ser Gln Asn Leu Gln Lys Ala Val Thr 515 520 525Thr Glu Thr Ile Lys Gly Leu Gln Pro Gly Lys Ser Tyr Leu Leu Asp 530 535 540Val Tyr Val Ile Gly His Gly Gly His Ser Val Lys Tyr Gln Ser Lys545 550 555 560Val Val Lys Thr Arg Lys Phe Cys 565126125PRTHomo sapiensmisc_featureIncyte Clone No 2493851 126Met Trp Leu Val Gly Pro Ser Phe Leu Ser Cys Pro Leu Gly Lys Val1 5 10 15Pro Pro Ala Gly Leu Leu Leu Ala Gly Ser Ser Gly Arg Gly Ala Arg 20 25 30Arg Pro Ala Thr Pro Arg His Trp Ser Ser Thr Thr Pro Gly Leu Arg 35 40 45Leu Glu Ala Pro Leu Cys Gln Leu Cys Pro Leu Gly Gly Thr Arg Gln 50 55 60Asp Cys Gln Pro Leu Ser Trp Gln Val Thr Ser Ala Phe Lys Leu Thr65 70 75 80Val Pro Ser Pro Phe His Ala Pro Pro Arg Ser Trp Ser Cys Leu Leu 85 90 95Leu Gly Ile Phe Pro Gly Gln Ala Leu Ala Leu Glu Pro Trp His Leu 100 105 110Phe Leu Gly Ser Met Leu Pro Arg Cys Asp Gly Glu Cys 115 120 125127196PRTHomo sapiensmisc_featureIncyte Clone No 2495719 127Met Ala Ala Leu Lys Ala Leu Val Ser Gly Cys Gly Arg Leu Leu Arg1 5 10 15Gly Leu Leu Ala Gly Pro Ala Ala Thr Ser Trp Ser Arg Leu Pro Ala 20 25 30Arg Gly Phe Arg Glu Val Val Glu Thr Gln Glu Gly Lys Thr Thr Ile 35 40 45Ile Glu Gly Arg Ile Thr Ala Thr Pro Lys Glu Ser Pro Asn Pro Pro 50 55 60Asn Pro Ser Gly Gln Cys Pro Ile Cys Arg Trp Asn Leu Lys His Lys65 70 75 80Tyr Asn Tyr Asp Asp Val Leu Leu Leu Ser Gln Phe Ile Arg Pro His 85 90 95Gly Gly Met Leu Pro Arg Lys Ile Thr Gly Leu Cys Gln Glu Glu His 100 105 110Arg Lys Ile Glu Glu Cys Val Lys Met Ala His Arg Ala Gly Leu Leu 115 120 125Pro Asn His Arg Pro Arg Leu Pro Glu Gly Val Val Pro Lys Ser Lys 130 135 140Pro Gln Leu Asn Arg Tyr Leu Thr Arg Trp Ala Pro Gly Ser Val Lys145 150 155 160Pro Ile Tyr Lys Lys Gly Pro Arg Trp Asn Arg Val Arg Met Pro Val 165 170 175Gly Ser Pro Leu Leu Arg Asp Asn Val Cys Tyr Ser Arg Thr Pro Trp 180 185 190Lys Leu Tyr His 195128214PRTHomo sapiensmisc_featureIncyte Clone No 2614153 128Met Val Leu Gly Gly Cys Pro Val Ser Tyr Leu Leu Leu Cys Gly Gln1 5 10 15Ala Ala Leu Leu Leu Gly Asn Leu Leu Leu Leu His Cys Val Ser Arg 20 25 30Ser His Ser Gln Asn Ala Thr Ala Glu Pro Glu Leu Thr Ser Ala Gly 35 40 45Ala Ala Gln Pro Glu Gly Pro Gly Gly Ala Ala Ser Trp Glu Tyr Gly 50 55 60Asp Pro His Ser Pro Val Ile Leu Cys Ser Tyr Leu Pro Asp Glu Phe65 70 75 80Ile Glu Cys Glu Asp Pro Val Asp His Val Gly Asn Ala Thr Ala Ser 85 90 95Gln Glu Leu Gly Tyr Gly Cys Leu Lys Phe Gly Gly Gln Ala Tyr Ser 100 105 110Asp Val Glu His Thr Ser Val Gln Cys His Ala Leu Asp Gly Ile Glu 115 120 125Cys Ala Ser Pro Arg Thr Phe Leu Arg Glu Asn Lys Pro Cys Ile Lys 130 135 140Tyr Thr Gly His Tyr Phe Ile Thr Thr Leu Leu Tyr Ser Phe Phe Leu145 150 155 160Gly Cys Phe Gly Val Asp Arg Phe Cys Leu Gly His Thr Gly Thr Ala 165 170 175Val Gly Lys Leu Leu Thr Leu Gly Gly Leu Gly Ile Trp Trp Phe Val 180 185 190Asp Leu Ile Leu Leu Ile Thr Gly Gly Leu Met Pro Ser Asp Gly Ser 195 200 205Asn Trp Cys Thr Val Tyr 21012988PRTHomo sapiensmisc_featureIncyte Clone No 2655184 129Met Ala Cys Phe Ser Phe Phe Leu Cys Phe Leu Val His Leu Leu Ile1 5 10 15Lys Met Asn Pro Val Thr Glu Ser Pro Ser Cys Leu Phe Ser Pro Pro 20 25 30Ser Glu Ser Ala Leu Ala Ser Gln Leu Ala Leu Ser Ala Ser Cys Asp 35 40 45Gln Arg Ala Pro Phe Ser Leu Ala Gly Val Val Ser His Asp Pro Gly 50 55 60Trp Pro Val Val Arg Leu His Arg Pro Leu Val Pro Glu His Ala Val65 70 75 80Phe Ser Gln Pro Ser Leu Gln Pro 85130260PRTHomo sapiensmisc_featureIncyte Clone No 2848362 130Met Pro Asp Pro Leu Phe Ser Ala Val Gln Gly Lys Asp Glu Ile Leu1 5 10 15His Lys Ala Leu Cys Phe Cys Pro Trp Leu Gly Lys Gly Gly Met Glu 20 25 30Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser Gly Ala 35 40 45His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 50 55 60Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp65 70 75 80Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 85 90 95His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 100 105 110Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr Leu Arg 115 120 125Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 130 135 140Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala145 150 155 160Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro Gly Glu 165 170 175Ser Glu Ser Phe Glu Asp Ala His Val Glu His Ser Ile Ser Arg Ser 180 185 190Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu 195 200 205Leu Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp 210 215 220Ala Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu225 230 235 240Leu Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly 245 250 255Leu Arg Asp Thr 260131295PRTHomo sapiensmisc_featureIncyte Clone No 2849906 131Met Gly Leu Pro Val Ser Trp Ala Pro Pro Ala Leu Trp Val Leu Gly1 5 10 15Cys Cys Ala Leu Leu Leu Ser Leu Trp Ala Leu Cys Thr Ala Cys Arg 20 25 30Arg Pro Glu Asp Ala Val Ala Pro Arg Lys Arg Ala Arg Arg Gln Arg 35 40 45Ala Arg Leu Gln Gly Ser Ala Thr Ala Ala Glu Ala Ser Leu Leu Arg 50 55 60Arg Thr His Leu Cys Ser Leu Ser Lys Ser Asp Thr Arg Leu His Glu65 70 75 80Leu His Arg Gly Pro Arg Ser Ser Arg Ala Leu Arg Pro Ala Ser Met 85 90 95Asp Leu Leu Arg Pro His Trp Leu Glu Val Ser Arg Asp Ile Thr Gly 100 105 110Pro Gln Ala Ala Pro Ser Ala Phe Pro His Gln Glu Leu Pro Arg Ala 115 120 125Leu Pro Ala Ala Ala Ala Thr Ala Gly Cys Ala Gly Leu Glu Ala Thr 130 135 140Tyr Ser Asn Val Gly Leu Ala Ala Leu Pro Gly Val Ser Leu Ala Ala145 150 155 160Ser Pro Val Val Ala Glu Tyr Ala Arg Val Gln Lys Arg Lys Gly Thr 165 170 175His Arg Ser Pro Gln Glu Pro Gln Gln Gly Lys Thr Glu Val Thr Pro 180 185 190Ala Ala Gln Val Asp Val Leu Tyr Ser Arg Val Cys Lys Pro Lys Arg 195 200 205Arg Asp Pro Gly Pro Thr Thr Asp Pro Leu Asp Pro Lys Gly Gln Gly 210 215 220Ala Ile Leu Ala Leu Ala Gly Asp Leu Ala Tyr Gln Thr Leu Pro Leu225 230 235 240Arg Ala Leu Asp Val Asp Ser Gly Pro Leu Glu Asn Val Tyr Glu Ser 245 250 255Ile Arg Glu Leu Gly Asp Pro Ala Gly Arg Ser Ser Thr Cys Gly Ala 260 265 270Gly Thr Pro Pro Ala Ser Ser Cys Pro Ser Leu Gly Arg Gly Trp Arg 275 280 285Pro Leu Pro Ala Ser Leu Pro 290 295132183PRTHomo sapiensmisc_featureIncyte Clone No 2899137 132Met Ala Ala Ser Met Ala Arg Gly Gly Val Ser Ala Arg Val Leu Leu1 5 10 15Gln Ala Ala Arg Gly Thr Trp Trp Asn Arg Pro Gly Gly Thr Ser Gly 20 25 30Ser Gly Glu Gly Val Ala Leu Gly Thr Thr Arg Lys Phe Gln Ala Thr 35 40 45Gly Ser Arg Pro Ala Gly Glu Glu Asp Ala Gly Gly Pro Glu Arg Pro 50 55 60Gly Asp Val Val Asn Val Val Phe Val Asp Arg Ser Gly Gln Arg Ile65 70 75 80Pro Val Ser Gly Arg Val Gly Asp Asn Val Leu His Leu Ala Gln Arg 85 90 95His Gly Val Asp Leu Glu Gly Ala Cys Glu Ala Ser

Leu Ala Cys Ser 100 105 110Thr Cys His Val Tyr Val Ser Glu Asp His Leu Asp Leu Leu Pro Pro 115 120 125Pro Glu Glu Arg Glu Asp Asp Met Leu Asp Met Ala Pro Leu Leu Gln 130 135 140Glu Asn Ser Arg Leu Gly Cys Gln Ile Val Leu Thr Pro Glu Leu Glu145 150 155 160Gly Ala Glu Phe Thr Leu Pro Lys Ile Thr Arg Asn Phe Tyr Val Asp 165 170 175Gly His Val Pro Lys Pro His 180133113PRTHomo sapiensmisc_featureIncyte Clone No 2986229 133Met Trp Arg Lys Pro Asp Val Leu Tyr Ser Val Ile Pro Val Thr Ser1 5 10 15Leu Phe Phe Leu Leu Ala Leu Asn Leu Pro Asp Val Phe Gly Leu Val 20 25 30Val Leu Pro Leu Glu Leu Lys Leu Arg Ile Phe Arg Leu Leu Asp Val 35 40 45Arg Ser Val Leu Ser Leu Ser Ala Val Cys Arg Asp Leu Phe Thr Ala 50 55 60Ser Asn Asp Pro Leu Leu Trp Arg Phe Leu Tyr Leu Arg Asp Phe Arg65 70 75 80Gly Asp Phe Arg Asn Asp Ile Phe Thr Arg Lys Gly Ser Tyr Cys Leu 85 90 95Asp Tyr Ser Ala His Gln Lys Phe Leu Val Val Gly Phe Phe Cys Cys 100 105 110Lys134160PRTHomo sapiensmisc_featureIncyte Clone No 3222081 134Met Gln Arg Val Ser Gly Leu Leu Ser Trp Thr Leu Ser Arg Val Leu1 5 10 15Trp Leu Ser Gly Leu Ser Glu Pro Gly Ala Ala Arg Gln Pro Arg Ile 20 25 30Met Glu Glu Lys Ala Leu Glu Val Tyr Asp Leu Ile Arg Thr Ile Arg 35 40 45Asp Pro Glu Lys Pro Asn Thr Leu Glu Glu Leu Glu Val Val Ser Glu 50 55 60Ser Cys Val Glu Val Gln Glu Ile Asn Glu Glu Glu Tyr Leu Val Ile65 70 75 80Ile Arg Phe Thr Pro Thr Val Pro His Cys Ser Leu Ala Thr Leu Ile 85 90 95Gly Leu Cys Leu Arg Val Lys Leu Gln Arg Cys Leu Pro Phe Lys His 100 105 110Lys Leu Glu Ile Tyr Ile Ser Glu Gly Thr His Ser Thr Glu Glu Asp 115 120 125Ile Asn Lys Gln Ile Asn Asp Lys Glu Arg Val Ala Ala Ala Met Glu 130 135 140Asn Pro Asn Leu Arg Glu Ile Val Glu Gln Cys Val Leu Glu Pro Asp145 150 155 160135865DNAHomo sapiensmisc_featureIncyte Clone No 443531 135attcctcaat tttccagtct cccttgagct aagtgtggcc ctatgactca cttccagcca 60tgaaaacaag tgcaaatctg ttaggagtat gttctggggc aatttttgct ctcctgatga 120agacaaaggc tgttgatcca ctgaacccac ccagacacta tgtggtttct tgaatgtcct 180acgtacattt tgatggatta cccaaggact atctgatgaa gaataataga gacatataaa 240tacatatggg ctacatcttg gcaaaataaa gtaatcctga agtaaattct aaggatgttc 300tgaattgaca cctcttaagc acaaccgaat gtcctggtgg ctttgcctcc cactggggct 360ttttggctct tgtttggccc cagcggctgc tgcagctctg tctgaattca cacaggagca 420acatgatggt gctcagccct cgccgaagtg tcttgctgaa gagttgggag atgcttggac 480tattcagata gaagccaact ggaagtacag ggcagtcaac acaaaccaga gaggcaaact 540tttggccagt gagacatgga aagggagaag aaatacattc ttctttctcc cctagagtga 600ggaccaacct gagtcccagt cacctggaat cccctcagac gagcgtccct tgagatccag 660cacatggcag ccagcgtgct gacgattcct tcctgcctac tggctccttc ttatttctgc 720ctccgtggaa ctgtattctc taatcaatat tagcacatac atattgcccc agactgtacc 780tcctgggaac ccaggataaa gcactatcta aacattttgt cttggaattg taataaactt 840caaaagaaaa atacaaaaaa aaaaa 865136706DNAHomo sapiensmodified_base(11)..(12)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 632860 136cggaccgtgg nnttggtaaa gcccatttcc gaggatttta gggagaccta ggtggggcag 60acactagaag tgtccagcct ccaagcccaa gagatgtggc cggcagggct gggcaggtcc 120ttgctggctc agcctgctct ttgctccttc atgggacccc agtggatcct gcagttctgc 180tcttggctgg aaccacgcca gcttcgctgg agctggactg agccgccttt tacattattg 240gactctctcg ggttgagagc tgcccaggac tcctgcagtt tcaccaccct tgttcctttg 300actcttgact catcattcat gaccgttaac gtggttccat ttgtatggac ttcttctttc 360ttcagagcat ttcagtatcc tgttacctcc ccatgcagaa caaagaatac tccacttttg 420atagatgggg ttaccaggat tcaggctaca tggcctgagg caaggtcaca acatgagtga 480cagaatgtgt cctggaagcc aggcatcctc tggggtgtat ttggggcgct caacaaggct 540tgatcgagct ttgggggtag atctagctat tccatgggga ttcttttcag aattgctgtt 600ttcggtaact aattccatga ccaggtccat ggcattggat gacattgcgc tacactgttg 660ctcacccggg tcacccgtcc tcacaggttg gatggcaagc atgttg 706137801DNAHomo sapiensmisc_featureIncyte Clone No 670010 137acttctacat gggcctcctg ctgctggtgc tcttcctcag cctcctgccg gtggcctaca 60ccatcatgtc cctcccaccc tcctttgact gcgggccgtt caggtgcaga gtctcagttg 120cccgggagca cctcccctcc cgaggcagtc tgctcagagg gcctcggccc agaattccag 180ttctggtttc atgccagcct gtaaaaggcc atggaacttt gggtgaatca ccgatgccat 240ttaagagggt tttctgccag gatggaaatg ttaggtcgtt ctgtgtctgc gctgttcatt 300tcagtagcca ccagccacct gtggccgttg agtgcttgaa atgaggaact gagaaaatta 360atttctcatg tatttttctc atttatttat taatttttaa ctgatagttg tacatatttg 420ggggtacatg tgatatttgg atacatgtat acaatatata atgatcaaat cagggtaact 480gggatatcca tcacatcaaa catttatttt ttattctttt tagacagagt ctcactctgt 540cacccaggct ggagtgcagt ggtgccatct cagcttactg caacctctgc ctgccaggtt 600caagcgattc tcatgcctcc acctcccaag tagctgggac tacaggcatg caccacaatg 660cccaactaat ttttgtattt ttagtagaga cggggttttg ccatgttgcc caggctggcc 720ttgaactcct ggcctcaaac aatccacttg cctcggcctc ccaaagtgtt atgattacag 780gcgtgagcca ccgtgcctgg g 801138664DNAHomo sapiensmodified_base(505)a, c, g, t, unknown, or othermodified_base(518)a, c, g, t, unknown, or othermodified_base(527)a, c, g, t, unknown, or othermodified_base(540)a, c, g, t, unknown, or othermodified_base(565)..(566)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 726498 138cggacgcgtg ggctggaagg agctctggag tcggaatcag gatgtggagg ctgagaagaa 60atctggctct accacctggg aaactggcat ggttgtattt gtcagtgttc agtcagggga 120gcagagccat gatgagtctt acggaaataa ggttaaaaca tatgcttgaa atttggcatg 180gcagacaagc cagagcttgt gaaaatctaa gaaaccaaac acgtgtagcc accaaagtgg 240aaccacaaaa gggaagatct acagaaattt gttgccttgc tgtagttcca ttaaatgagg 300ttgtgcagtc aagcatcttg tggtgggtct ggagctgttg ccagcatcag gaagacaagc 360tgggtgctaa gtgaagaaat acacaatgta gaaactgtca ggcatctctg cccctggact 420tcaccatatc tgatgatgtt ctcagagtca gggcactgct tcacttttcg cttccaaatc 480tcacacaaaa ttctctgtta ggcancccca gcttagancc ttacaantga gggggatcan 540ggaaatggag tacccagata cccanngtga tatactttta tgccctcagt ttcttatctt 600tcagtgggga taatatcctc ggatacaaaa gagtgtacat atataccctg tatttggtaa 660acta 6641391241DNAHomo sapiensmisc_featureIncyte Clone No 795064 139ccaggcaata tctcaggata tggaagtttc tgggtttatt tacccctcag tgcccagagt 60taaagtttca gaagagactt gtgcacataa gggcttcatc tcaagtgtat tgcagtaatg 120gctgaatcgg ggttaacatc ccttccaggc acagcgagtt ggttctgctt tttgcctgta 180agccaaagaa aagccacatc taaaaagcta ctactaaaag ccagaaagaa aagtggattt 240gaactcagtg tcacagactc ttctgagtgt tttagggtca cagctagtgt aagaggcatg 300aagaatagac atgcaaaagg gaacgggtgc accagagacc cctgttttgg ctgacagacc 360atatgtccca ccagctgggg aatctgacaa gaggacatag gtggcactct ttttttaaag 420ctatttattg tatctatttt taaataaaat tgcccatcct cattcagctc ttagaacaaa 480agcaaaaaac cctgtaaatc aggagatata agcacatctg cacccagaat aggcccatat 540gatagggcaa ccctgagctt aaacaatgac atcttcaagg gtagaactaa tctgaaaccc 600ccttccagcc tctggaagac actggcctgc atcagttaga gtcagagcaa gtgtcacttc 660acagggaaaa gaaggattat atagacttcc tatccctaga gtttataaat gtcaactata 720taaaaaaagc tcaaaacagt gttaaaggaa tgaacagtag aattttaata ggctgtccaa 780agaagccagg tctgctgtgg gcaagtatag cctaacccta gtcttgtaaa ataagccaga 840aagggttact gagccacctt aagctagtac ctatatagta ggcaaaaagt acagaaatag 900atgcaataag tgtggtgagt ctttgagcct acgagtcatg ccaccagcca taagttgacc 960tatcacttga gaacctcctc agcaaagatg ccagaaaaca ttcaatcaag ttggcaaatg 1020acacagggag ctggccctct gaccatcttc ctggcaaacc tggactggaa gggccatttg 1080cagcactgtc ctggagctaa tacactgttt cactgcctct gccatataat gatgccagca 1140ctagccagct ggtgggtatt tggaggaatc ctgcatgagg attgcccaat aaggggcagg 1200tacacatacc tggcaaagtg atgatgatgt gaattgtttc c 1241140750DNAHomo sapiensmodified_base(570)a, c, g, t, unknown, or othermodified_base(641)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 924925 140tggagtgggg agaagagcat acgccaggag cctcctgcct caaagtgctc ccctaagtct 60tcttcctcct gtgctgacct cagggtggtc tgacccttcc ctcggtgtgg gggatgtggc 120cctctcaggt gcccctactt gctttctgct tccttctggt gaagtccacc tccaacatta 180acctgcccac cccacccccg tcatccctgg agaattccag ctttgtcgta tctcagagag 240ggaatctaat tgtttttggg gggcaaaaga aagcaacgtt taggtatcac ttctacttgg 300accgcatgcc tttttatagc caaatttctg tgtatttcgt aaatggattt cgcgttaatg 360gatatttatg taataactag acttctcaga ttattgtgag aagggtcagg ttggaagggg 420tgtaggaaga cgggtgaggg gtagtttttt tctgtcctag tttttttttt ttttattgtc 480atctctgagg tggactttgt cacctgtggt tattggggcc aagtggactc agctccgggg 540gagaaggctt ctctgccatt tcggtccaan ggtgactgac acaggcgtac tttttgggac 600tgtggaagca tcagatgcca gcactgactt cagaccagca nttcgggcta gaggaagatg 660ggacctttca ggatggaaat accttggact ttcttttggt ccctcggaaa cttgggcttt 720ctctaccgac ttgcccagat ttcatttcac 7501411235DNAHomo sapiensmisc_featureIncyte Clone No 962390 141ccctcaggca gcccctccac aggacccctc tcctgcctgg acagctctgc tggtctcccc 60gtcccctgga gaagaacaag gccatgggtc ggcccctgct gctgcccctg ctgctcctgc 120tgcagccgcc agcatttctg cagcctggtg gctccacagg atctggtcca agctaccttt 180atggggtcac tcaaccaaaa cacctctcag cctccatggg tggctctgtg gaaatcccct 240tctccttcta ttacccctgg gagttagcca tagttcccaa cgtgagaata tcctggagac 300ggggccactt ccacgggcag tccttctaca gcacaaggcc gccttccatt cacaaggatt 360atgtgaaccg gctctttctg aactggacag agggtcagga gagcggcttc ctcaggatct 420caaacctgcg gaaggaggac cagtctgtgt atttctgccg agtcgagctg gacacccgga 480gatcagggag gcagcagttg cagtccatca aggggaccaa actcaccatc acccaggctg 540tcacaaccac caccacctgg aggcccagca gcacaaccac catagccggc ctcagggtca 600cagaaagcaa agggcactca gaatcatggc acctaagtct ggacactgcc atcagggttg 660cattggctgt cgctgtgctc aaaactgtca ttttgggact gctgtgcctc ctcctcctgt 720ggtggaggag aaggaaaggt agcagggcgc caagcagtga cttctgacca acagagtgtg 780gggagaaggg atgtgtatta gccccggagg acgtgatgtg agacccgctt gtgagtcctc 840cacactcgtt ccccattggc aagatacatg gagagcaccc tgaggacctt taaaaggcaa 900agccgcaagg cagaaggagg ctgggtccct gaatcaccga ctggaggaga gttacctaca 960agagccttca tccaggagca tccacactgc aatgatatag gaatgaggtc tgaactccac 1020tgaattaaac cactggcatt tgggggctgt ttattatagc agtgcaaaga gttcctttat 1080cctccccaag gatggaaaaa tacaatttat tttgcttacc atacacccct tttctcctcg 1140tccacatttt ccaatctgta tggtggctgt cttctatggc agaaggtttt ggggaataaa 1200tagcgtgaaa tgctgctgac acttaaaaaa aaaaa 12351421834DNAHomo sapiensmisc_featureIncyte Clone No 1259405 142gacggaagtg cgggcggagg atccccagcc gggtcccaag cctgtgcctg tgcctgagcc 60tgagcctgag cctgagcccg agccgggagc cggtcgcggg ggctccgggc tgtgggaccg 120ctgggccccc agcgatggcg accctgtggg gaggccttct tcggcttggc tccttgctca 180gcctgtcgtg cctggcgctt tccgtgctgc tgctggcgca gctgtcagac gccgccaaga 240atttcgagga tgtcagatgt aaatgtatct gccctcccta taaagaaaat tctgggcata 300tttataataa gaacatatct cagaaagatt gtgattgcct tcatgttgtg gagcccatgc 360ctgtgcgggg gcctgatgta gaagcatact gtctacgctg tgaatgcaaa tatgaagaaa 420gaagctctgt cacaatcaag gttaccatta taatttatct ctccattttg ggccttctac 480ttctgtacat ggtatatctt actctggttg agcccatact gaagaggcgc ctctttggac 540atgcacagtt gatacagagt gatgatgata ttggggatca ccagcctttt gcaaatgcac 600acgatgtgct agcccgctcc cgcagtcgag ccaacgtgct gaacaaggta gaatatgcac 660agcagcgctg gaagcttcaa gtccaagagc agcgaaagtc tgtctttgac cggcatgttg 720tcctcagcta attgggaatt gaattcaagg tgactagaaa gaaacaggca gacaactgga 780aagaactgac tgggttttgc tgggtttcat tttaatacct tgttgatttc accaactgtt 840gctggaagat tcaaaactgg aagcaaaaac ttgcttgatt tttttttctt gttaacgtaa 900taatagagac atttttaaaa gcacacagct caaagtcagc caataagtct tttcctattt 960gtgactttta ctaataaaaa taaatctgcc tgtaaattat cttgaagtcc tttacctgga 1020acaagcactc tctttttcac cacatagttt taacttgact ttcaagataa ttttcagggt 1080ttttgttgtt gttgtttttt gtttgtttgt tttggtggga gaggggaggg atgcctggga 1140agtggttaac aacttttttc aagtcacttt actaaacaaa cttttgtaaa tagaccttac 1200cttctatttt cgagtttcat ttatattttg cagtgtagcc agcctcatca aagagctgac 1260ttactcattt gacttttgca ctgactgtgt tatctgggta tctgctgtgt ctgcacttca 1320tggtaaacgg gatctaaaat gcctggtggc ttttcacaaa aagcagattt tcttcatgta 1380ctgtgatgtc tgatgcaatg catcctagaa caaactggcc atttgctagt ttactctaaa 1440gactaaacat agtcttggtg tgtgtggtct tactcatctt ctagtacctt taaggacaaa 1500tcctaaggac ttggacactt gcaataaaga aattttattt taaacccaag cctccctgga 1560ttgataatat atacacattt gtcagcattt ccggtcgtgg tgagaggcag ctgtttgagc 1620tccaatgtgt gcagctttga actagggctg gggttgtggg tgcctcttct gaaaggtcta 1680accattattg gataactggc ttttttcttc ctctttggaa tgtaacaata aaaataattt 1740ttgaaacatc catcagtgta tctatctatg tctcctagtt ttttcctcct ccctcttttg 1800ctgtataatg agagagaaga tctgatgaga taac 18341431722DNAHomo sapiensmisc_featureIncyte Clone No 1297384 143tacgagaccc ggccgcaccc cgagtcacac aggcccccgg ggccacggcg ttcgtctctc 60ctgtgctgtc ctcaggcctc cgctcctgtt ttggtggccc aggctctccc ctgccccatc 120ctcgctcccc cacctccttg ggtcatgccc acccaccctt tcctgcctcc tccgtgtgaa 180gacatccaac atccacgtga cttttccagc tccattttta aacagtgact gagattctag 240aaaaactggc tgctaactgg cctgagccag gcaacactga ttccaatccc tcctcctttt 300ttaagttatt tgatggaaga ctcacctaat ttgtgacctg aaactgttga agaaatagag 360aggagggggc ccgttgatta cagagagcat ttgggatttt gtttggtttg gagatgatgc 420ctaggttact gggtttgggg ggattgtttt cttttggggg ccttcccctt ttactccttt 480tcttccagag atcaagagct tctcttgcat cttcttccac tgggctctgg attaatcaat 540tacccaaagg ctgcacctgc cgtgttgtct gggcttgcat cccagatgtg ttggagtatg 600catggatgta gtgcttttta gaggagccac tgggcaaggc caccaagaac aaatgcatga 660cattttatag ccaaggacgc ctcgctaaag tcttatgggc gtcccctggg gttggggggg 720cacaaggttt tggaggaaga agacaacttc cctcattcca tcatcaccat ctctttctca 780ctaggttctt tctagttttc aagcaatagt tctagcctgc cttggacaag ggggccccag 840ttaaacaaac tacccatcca tgaggtgcca ggcagtcaaa aacagaagct tccccgactt 900gtgagtccct gagatgtgct cttgttgttt ggcatttggg gtgacaggga gtgacccaga 960ggccaccact gcttttcatg caggagttac agacactggt ttcttggaaa atggagagaa 1020gcgcactttg cacagacgtc gtcaattaag tcccaatttg ccacttggta ttgagtacac 1080tggaccctga ccactggctc ttgggcaaac gtccttcctc acggggcgcc tccgccaagc 1140cggcccagct gcacccctcc cttcctggag ggatggccag ggaaggagaa aacagagaac 1200tgacaccttt gaaaccacag aatgtgttac atgcagactc gctcaagggc ataagttatt 1260gtgaacgttt ttgccaatca ctgctcaaca gccctgctag attttgtatg atgctgaatt 1320attatgcaga ctaattccac ccagttgaga cacaccatgc ttgttcactt gtatttattg 1380aaactgtgga ttcttgcccg tgctgtccct tgtatttact ttaagcactg atcacttatc 1440attcattcgg tatggttttc cctgtccctt gtacacattc tggtatgaat ttgtaaaaat 1500aacctgctac aaattggttg aatgtttctg tctgtggtgc gaaccagcat taacggatgg 1560ggcacgtgcc caactgagga acaggagaag aaatcaccaa tttgggctct cagagctaag 1620acacacttat tgattctgtt gcacattttg cactggttta tggcgattgt tttcttggac 1680ggatagtgta aaataaactt ctctgttctc taaaaaaaaa aa 17221441741DNAHomo sapiensmisc_featureIncyte Clone No 1299627 144ttcgctccaa gcctcaggcc accggcttgg atggacgctc cgaggctacc cgtgcgtcca 60ggggtcttgc ttccgaagtt ggtcctgctc tttgtctacg cagatgattg ccttgctcag 120tgtggcaaag attgcaaatc ttactgctgt gatggaacca cgccctactg ttgctcctac 180tacgcttata ttgggaatat cctctcgggc actgcaattg cgggcattgt ttttggaata 240gtatttatca tgggggtcat tgctgggatt gccatatgca tctgcatgtg catgaagaac 300cacagggcga cccgcgtggg catcctcagg acgactcaca tcaacaccgt ctcctcctat 360cctggaccac caccctacgg tcacgaccac gagatggaat actgtgcaga cttgcctcct 420ccatactccc ccaccccaca gggtccagca cagcgttctc caccccctcc ttatcctgga 480aacgcaagga aataatctat ctcccagaac agaacatgtg ccaatgggcg atcttgcctg 540gaataaaatg cctctactca gaaacaggca ggaaagaatt gctccaagga atactttttg 600gggtcagata atgtgtcagg tggaatatcc ctgctaggag atataggatt tctactctgc 660tcaaagctga ccccatctgg agtattaatg tttggttcta tggaaccaca ttttaagaga 720tctgctgatc cacctaagca cattcaggga agagtaatgt aattgacaaa atatctgata 780atcatgttgt ttaagggcta ggtgaagaaa gtttcagtat tgatcctgga aaaaaagaag 840atctaagtag gatgggagaa tgatttggcc cacacaagga agcaacttta ttctatatag 900ctttaaaagt cagaactaga attgttcatt ctttcattca tcaataaatg tattttgagt 960gcctaagagt ttactatgtg cctagcactg tttgaggtcc tgatggaagt tacaggatgg 1020gtactctggt tttagtacaa gaaagagcaa tgactagatt gctttgtgaa gctcttggta 1080gagacacgct ccagaaggga taacaaaatc aaatagtaga tgggttcatt gggcctcaga 1140agttctgctc gtattttagg tgggtgtgaa gtgaatttct atatgtccag gagtgaatac 1200aacagaaaga gttggatctt atttatttaa ttagggagtt aaaacaagac caaaaagact 1260caacagccgc ttgaagccaa gaactcttca atgccagcta ctgccaccta aaaatcatct 1320ggctttatag tggatcagaa taaaggttat tctaactgtg gggagaaaaa aaaaattgta 1380tcaagttcca caggtagcag acacttcact tccaagtaaa agatgagaaa tcaattattc 1440ccacaggatt ttaggtcagg gagcaaaaat ctcagaactt gaccatgaag atacacaaca 1500gactcgcaaa aataaagtgg gaaatgaagt tcagattccc ttctgtagat ttccttaaaa 1560ctattatttt tttcttcttc gtaaaatttt gataatctgt tctcttaaaa aagttaatga 1620cacaattaag atactgacat caaattgttg ccttttacca aaatgcaaat tttatgaagt 1680gcctaccttt atatgtataa agcatttaat aaataattct aatgtgccat aaaaaaaaaa 1740a

1741145997DNAHomo sapiensmodified_base(973)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 1306026 145ggacaaccgt tgctgggtgt cccagggcct gaggcaggac ggtactccgc tgacaccttc 60cctttcggcc ttgaggttcc cagcctggtg gccccaggac gttccggtcg catggcagag 120tgctacggac gacgcctatg aagcccttag tccttctagt tgcgcttttg ctatggcctt 180cgtctgtgcc ggcttatccg agcataactg tgacacctga tgaagagcaa aacttgaatc 240attatataca agttttagag aacctagtac gaagtgttcc ctctggggag ccaggtcgtg 300agaaaaaatc taactctcca aaacatgttt attctatagc atcaaaggga tcaaaattta 360aggagctagt tacacatgga gacgcttcaa ctgagaatga tgttttaacc aatcctatca 420gtgaagaaac tacaactttc cctacaggag gcttcacacc ggaaatagga aagaaaaaac 480acacggaaag taccccattc tggtcgatca aaccaaacaa tgtttccatt gttttgcatg 540cagaggaacc ttatattgaa aatgaagagc cagagccaga gccggagcca gctgcaaaac 600aaactgaggc accaagaatg ttgccagttg ttactgaatc atctacaagt ccatatgtta 660cctcatacaa gtcacctgtc accactttag ataagagcac tggcattgag atctctacag 720aatcagaaga tgttcctcag ctctcaggtg aaactgcgat agaaaaaccc gagagttgga 780agcaccagag agtgggatat gatgcatttg aaaaaaattt agtattaatc acaatgcaca 840ggcacttcta gtgacacagc acccagctat agagagatat gaaggggtac gagctcgaat 900tcgaatcatg tcatagctgt ttcctgtgtg aattggtatc gctcacaatg cacacacata 960cgagcggaag ctnaattggt aagcgggggt gccatga 997146981DNAHomo sapiensmisc_featureIncyte Clone No 1316219 146gttttaaatt tacttaataa atataaaata ttgtatgttc ttaacttgaa gctcatattt 60tcaagtaatt ccttgtctgg aattttctgt tgatctcatg ggtactaaga aacgaaatat 120tctgttcatt ttcattttta aagaatatcg ataacttgat gaccccagaa ggagttggcc 180ttaccactgc cttacgtgtt ctctgtaatg ttgcatgccc accacctcct gttgaaggtc 240aacagaaaga tctgaaatgg aatcttgccg ttattcagct tttttctgct gaaggaatgg 300acacgtttat tcgagttctg caaaaattga acagtattct gactcagcct tggaggctcc 360atgtcaacat ggggactacc cttcacagag ttactactat ttcaatggct cgctgcacac 420tcactcttct taaaactatg ttaacggaac tcctgagagg tggatccttt gagtttaagg 480acatgcgtgt tccttcagcg cttgttactt tacatatgct cctgtgctct atccccctct 540caggtcgttt ggatagtgat gaacagaaaa ttcagaatga tatcattgat attttactga 600cttttacaca aggagttaat gaaaaactca caatctcaga agagactctg gccaataata 660cttggtcttt aatgttaaaa gaagttcttt cttcaatctt gaaggttcct gaaggatttt 720tttctggact catactcctt tcagagctgc tgcctcttcc attgcccatg caaacaactc 780aggtatcact tccatataac atgcatctta taaatgactg cagtaacact ttttaaaaag 840ccagtgattt tgttaaaaaa caaaaaccct catctccctt cctcccaaaa agacataaaa 900taaccggatg agggggagat aaaactgaaa caagttggtc attgaggaaa tatgggggta 960aaaattttaa ataaattttt g 981147526DNAHomo sapiensmisc_featureIncyte Clone No 1329031 147ctgcaggccc acctgtctgc aacccagctg aggccatgcc ctccccaggg accgtctgca 60gcctcctgct cctcggcatg ctctggctgg acttggccat ggcaggctcc agcttcctga 120gccctgaaca ccagagagtc cagcagagaa aggagtcgaa gaagccacca gccaagctgc 180agccccgagc tctagcaggc tggctccgcc cggaagatgg aggtcaagca gaaggggcag 240aggatgaact ggaagtccgg ttcaacgccc cctttgatgt tggaatcaag ctgtcagggg 300ttcagtacca gcagcacagc caggccctgg ggaagtttct tcaggacatc ctctgggaag 360aggccaaaga ggccccagcc gacaagtgat cgcccacaag ccttactcac ctctctctaa 420gtttagaagc gctcatctgg cttttcgctt gcttctgcag caactcccac gactgttgta 480caagctcagg aggcgaataa atgttccaac ctggtaaaaa aaaaaa 5261482090DNAHomo sapiensmisc_featureIncyte Clone No 1483050 148gaggcgcggg gagagtaggg tgctgtggtc tgagctagag ggtgaagctg gcggagcagg 60aggatgggcg tatgcaggtg atagactaga gaacaagacc tctgtctccg tagcatcctg 120gagcagtctg aatgccagaa tggataaccg ttttgctaca gcatttgtaa ttgcttgtgt 180gcttagcctc atttccacca tctacatggc agcctccatt ggcacagact tctggtatga 240atatcgaagt ccagttcaag aaaattccag tgatttgaat aaaagcatct gggatgaatt 300cattagtgat gaggcagatg aaaagactta taatgatgca ctttttcgat acaatggcac 360agtgggattg tggagacggt gtatcaccat acccaaaaac atgcattggt atagcccacc 420agaaaggaca gagtcatttg atgtggtcac aaaatgtgtg agtttcacac taactgagca 480gttcatggag aaatttgttg atcccggaaa ccacaatagc gggattgatc tccttaggac 540ctatctttgg cgttgccagt tccttttacc ttttgtgagt ttaggtttga tgtgctttgg 600ggctttgatc ggactttgtg cttgcatttg ccgaagctta tatcccacca ttgccacggg 660cattctccat ctccttgcag gtctgtgtac actgggctca gtaagttgtt atgttgctgg 720aattgaacta ctccaccaga aactagagct ccctgacaat gtatccggtg aatttggatg 780gtccttctgc ctggcttgtg tctctgctcc cttacagttc atggcttctg ctctcttcat 840ctgggctgct cacaccaacc ggaaagagta caccttaatg aaggcatatc gtgtggcatg 900agcaagaaac tgcctgcttt acaattgcca tttttatttt tttaaaataa tactgatatt 960ttccccacct ctcaattgtt tttaattttt atttgtggat ataccatttt attatgaaaa 1020tctattttat ttatacacat tcaccactaa atacacactt aataccacta aaatttatgt 1080ggtttacttt aagcgatgcc atctttcaaa taaactaatc taggtctaga cagaaagaaa 1140tggatagaga cttgacacaa atttatgaaa gaaaattggg agtaggaatg tgaccgaaaa 1200caagttgtgc taatgtctgt tagacttttc agtaaaacta aagtaactgt atctgttcaa 1260ctaaaaactc tatattagtt tctttgggaa acctctcatc gtcaaaactt tatgttcact 1320ttgctgttgt agatagccag tcaaccagca gtattagtgc tgttttcaaa gatttaagct 1380ctataaaatt gggaaattat ctaagatcat tttccctaag cattgacaca tagcttcatc 1440tgaggtgaga tatggcagct gtttgtatct gcactgtgtc tgtctacaaa aagtgaaaaa 1500tacagtgttt acttgaaatt ttaactttgt aactgcaaga attccagttc agccgggcga 1560ggattagtat tatttttaac tctccgtaag attttcagta ccaccaaatt gttttggatt 1620ttttttcttt cctcttcaca taccagggtt attaaaagtg tgctttcttt ttacattata 1680ttacagttac aaggtaaaat tcctcaactg ctatttattt attccagccc agtactataa 1740agaacgtttc accataatga ccctccagag ctgggaaacc taccacaaga tctaaagttc 1800tggctgtcca ttaacctcca actatggtct ttatttcttg tggtaatatg atgtgccttt 1860ccttgcctaa atcccttcct ggtgtgtatc aacattattt aatgtcttct aattcagtca 1920tttttttata agtatgtcta taaacattga actttaaaaa acttatttat ttattccact 1980actgtagcaa ttgacagatt aaaaaaatgt aacttcataa tttcttacca taacctcaat 2040gtctttttta aaaaataaaa ttaaaaatga aaagagactc aaaaaaaaaa 20901492403DNAHomo sapiensmisc_featureIncyte Clone No 1514160 149gggagagagc agcagagacc tcatcagcag accaaggaag tggtgggtgc tccccctccc 60taagctccag ggtccctgaa tcttctgaaa tctcaaatga gtggaggcct cctggggtgg 120cctgtcctgc aggggccctg gaatgggggc aagcagctgg gtgggcagaa tgcagagtag 180actcggggga ggatcctttc actttccgct tccccttctg atgcatggag gatggtgtga 240gcttttcagc aggcccggaa aggtacgcag gtgacgcctt agcagccccg cagctggtgc 300tctgccccgc ggtactggcg ccatcagggc ctcccttgcc cgcctgagag cagcagcagt 360ctctgtcatc ccgtcgcccc ttacccccca ccccaggcca ctgggcccct cccacaccac 420ctggggagct gagaagagga ggctggagta agggaggact tgatcatcca agaaatactt 480tttattgctg ggagtcttct gaacctcacc aaactgaggc cagagctgag ctcctggggg 540agttaattca gaggggagag gccagcacct ccctcctcca tcgctcgctg tgtgccttaa 600actccatctc atgtccctcc ccatcccctg gctttccctc cctccttgcc ccatcctggg 660ccagccagca gggctcctcc tctggctctt cagacctttc agccagtgct gtcagtgccc 720ctgggaggga agggcatccc tgaggcaccc gaatggtccc tcagggtgca gggaggcaga 780agcctggcca cagaggagcc tcctaaggca gcagctgcag caagcgcacc ctctccccac 840tctccccacg ccagagcggc ttccagagca gatgctgttt ccatcctcct cgtcaaaacc 900attctcgctg ctgagcttga caatctgggc aaggcttgtg gggcgcttga caaacagaat 960ctgccctgtg ccgcctggtt ccgtggcctc cagcatgagc ctgcaggcag ggcgctgcgg 1020gaacccagtt gtgctgcccc agcccatgcc tccgggtctg ctgtgcatga atgagtgctc 1080acttgtcccg ggtttaggac gtggtcaagt gaacagcagg gtctaactgt gcttacttag 1140cccagttcaa acagaacaaa ggaaaaatat agaaagcaac atctgttgat catttaggtt 1200tttttttaaa ccaccatgtc actttgagtc cttcatgggt ttttgaacag catttatcaa 1260gaagaaaatg tgggcttttt cccctctccc gtgttttgtt tgtcctgtag atagagggag 1320gaaagccgtg cagtggcagg cgggaccccc tctggtggcg ggaccccctc ttgcggtggt 1380cttgcggggc cagccgggac ctgtcacttt attatttaag gagtgtgtgt gtagagtcgc 1440tggcttatta acagtattgt gtgtgggttg ggtttttagt ttgttccttc tttttgaagt 1500cccttcattt caatccttga ctctctctcc ccttcccttg cccagctctg ttgaatgctg 1560ctgtgcgcgt gtgagggccg ctctgcacac agggcccttg ggttgtgtga actgaaattc 1620tccctgtatt tgtgagactc gcaggagtcc ccatctgtag cacaggcaat gccagtgcca 1680tgctgcagcc tcagaaacca ggcctctcac tccagcagca ggcagaaccg tgtctgtggt 1740cgggtgctgt ccacagctct gtctgccttg ttcttgggct tgagctggat agaggtgggg 1800tctcttcacc ttccctgaat tcagaacaga ccctgtgcct ggccccagtg tgcccaggca 1860attccccagg ccctcattgg gagcccttgg tgttctgagc agcagggccc aggcagcaca 1920tgagcagtgc ccaggggctc cctgcgtgag gacggcaagg tgcgatgtat gtctaactta 1980ttgatggcag gcagccccct gtgcccccta agcctggccc tggttattgc tgagctctgt 2040gctcagtgct gcggcctggc cgtggctcgt ctgttccttt ggggggcccg ggcgggttgt 2100gggaatcagt cttcacagac agacgtgagc caggcggagg actcgttcct tgcagaggtc 2160agtcctcacc tgcaggtgtc ggggtggggg ggggcaagga ggggcaggca cacaccatgt 2220ctgacctgaa cccgattctg gggagcatct tcccgctccg gccccacgac ctccacaggg 2280ttacattgta atatatatgc cccagctaac ctgtctgatg gtggcatctt cctgcagaca 2340tttcaaacat gtaactttta tatgaaaaaa aataaacaca gatgaaagct gaaaaaaaaa 2400aaa 2403150431DNAHomo sapiensmisc_featureIncyte Clone No 1603403 150ggccaccggg acttcagtgt ctcctccatc ccaggagcgc agtggccact atggggtctg 60ggctgcccct tgtcctcctc ttgaccctcc ttggcagctc acatggaaca gggccgggta 120tgactttgca actgaagctg aaggagtctt ttctgacaaa ttcctcctat gagtccagct 180tcctggaatt gcttgaaaag ctctgcctcc tcctccatct cccttcaggg accagcgtca 240ccctccacca tgcaagatct caacaccatg ttgtctgcaa cacatgacag ccattgaagc 300ctgtgtcctt cttggcccgg gcttttgggc cggggatgca ggaggcaggc cccgaccctg 360tctttcagca ggcccccacc ctcctgagtg gcaataaata aaattcggta tgctgaattc 420aaaaaaaaaa a 4311512109DNAHomo sapiensmisc_featureIncyte Clone No 1652303 151tttgtagcca agtgggagct attttctttt ttgtgcatat agatatttct taaatgaagc 60tgctttcttg tcttttattt ctaaaagccc ccttataccc cactttgtgc agcaaagatc 120cccgtgcagg tcacagcctg atttgtggcc aggctggaca aattcctgag gcacaacttg 180gcttcagttc agatttcaag ctgtgttggt gttgggacca gcagaaggca aacgtccagc 240caacacacag gactgtaaga ggactctgag ctacgtgccc tgtgaagacc cccaggcttt 300gtcataggag gtcgttcagc ttccccaaag tcagaggtga tttgatttgg ggaagactga 360atattcacac ctaagtcgtg agcatatcct gagttttact tccttatggc ttgccctcca 420agttctctct ctcatacaca cacacaccct tgctccagaa tcaccagaca cctccatggc 480tccagctatg ggaacagctg cattggggct gcctttctgt ttggcttagg aacttctgtg 540cttcttgtgg ctccactcgc gaggcagctc ggaggtgtgg actccgattg ggctgcaggc 600agctctggga cggcacaggg cgggcgctct gatcagctcg tgtaaaacac accgtcttct 660tggcctcctg gccagtcttt ctgcgaatag tcctctccct ggccagttga atgggggaag 720ctgctggcac aggaaggaga ggcgatcccg gctgaggctt aggaaattgc tggagccggc 780tccaagcaga taattcactg gggaggtttt cagagtcaaa catcattctg cctgtgttgg 840gggccaggtg tgtcacacaa gcatctcaaa gtcaaaagcc atctggggct gctgcttctc 900tttctcaggc tctggggaaa ggaatctccc tctcctctca cttgattcca agtgtggttg 960aattgtctgg agcactggga ctttttttct cttttccttg atggaccaac agtgcaaatg 1020caatctcgcc atttaacttt caggtcgatt tcctttcctg atcagacatc tttgtgcccc 1080ctttaggaag gaaaagaata cacctacgat gtgccaggca ctgtgttagg cgcttttata 1140tagatcctcg ttaggatgag actaagggat gaggacatct ctttataaaa ggcccctaag 1200taatggataa acagaaacac ttagaggtga gaaggtctgt cttcaagatc caaggtaaga 1260ttgccttcag tctgatgttt gttctcaagg acttatcccc tacaatattc tcccactcca 1320tacttctcct tctaccccac catgtgctcc cgtgcactcc tcagatggtc agaggggtaa 1380cccaagtcct tagagaattt ggggaccaat agaatatgtg atgtgtgaat tttctttaaa 1440aaacttaagg agtctttgct accttctgct tgttgagttg ttttggcatt catattaaaa 1500gccagcatct cactatttat tgacaggttg ggctgtgtgt gtgcgcatgt gtgtatacat 1560ttccaggcgt gcctgtgtcc tgtagctttt taaaaggaaa cccagtcatc ccactatgaa 1620tctggcatct tcttatgctt ctagtgtttt ggccatacat caaccaaggg gtttaattta 1680tccaatgctt gacgacatgt tcaggagggg ctggatcaaa ttttgagagg gttatgggaa 1740agggaggggg agaagaaatt gacatttatt ttattattta ttttaaatgt ttacatcttc 1800tttatgttgt atcaagcctg aatagaaact gatagcatta aaatactccg ttcctctctc 1860tcttctcgct tccttttttt ttttttttaa atttaggata acacattttt gtttctaaag 1920tgatttgtga tttgtgctgt ataaactgta taaaaggttc tgtttttaaa ggtggatttt 1980cattcctctg gggacagtgg tcgccaagac atctacattg taagagaaca cagtggaaga 2040tcctgtcctg attctcaaaa attattttct ctgtatgatt aaaagtttat tccatttaaa 2100aaaaaaaaa 21091521114DNAHomo sapiensmisc_featureIncyte Clone No 1693358 152ggccggagca gctgtcaggc tgaagtcctg cgagcgacgc gcggcggggc ggcgagagga 60aacgcggcgc cgggccgggc cgggccctgg agatggtccc cggcgccgcg ggctggtgtt 120gtctcgtgct ctggctcccc gcgtgcgtcg cggcccacgg cttccgtatc catgattatt 180tgtactttca agtgctgagt cctggggaca ttcgatacat cttcacagcc acacctgcca 240aggactttgg tggtatcttt cacacaaggt atgagcagat tcaccttgtc cccgctgaac 300ctccagaggc ctgcggggaa ctcagcaacg gtttcttcat ccaggaccag attgctctgg 360tggagagggg gggctgctcc ttcctctcca agactcgggt ggtccaggag cacggcgggc 420gggcggtgat catctctgac aacgcagttg acaatgacag cttctacgtg gagatgatcc 480aggacagtac ccagcgcaca gctgacatcc ccgccctctt cctgctcggc cgagacggct 540acatgatccg ccgctctctg gaacagcatg ggctgccatg ggccatcatt tccatcccag 600tcaatgtcac cagcatcccc acctttgagc tgctgcaacc gccctggacc ttctggtaga 660agagtttgtc ccacattcca gccataagtg actctgagct gggaagggga aacccaggaa 720ttttgctact tggaatttgg agatagcatc tggggacaag tggagccagg tagaggaaaa 780gggtttgggc gttgctaggc tgaaagggaa gccacaccac tggccttccc ttccccaggg 840cccccaaggg tgtctcatgc tacaagaaga ggcaagagac aggccccagg gcttctggct 900agaacccgaa acaaaaggag ctgaaggcag gtggcctgag agccatctgt gacctgtcac 960actcacctgg ctccagcctc ccctacccag ggtctctgca cagtgacctt cacagcagtt 1020gttggagtgg tttaaagagc tggtgtttgg ggactcaata aaccctcact gactttttag 1080caataaagct tctcatcagg gttaaaaaaa aaaa 11141532192DNAHomo sapiensmisc_featureIncyte Clone No 1707711 153ggaactcatg agccaacagc tgaagaaacc cattgctaca gcttccagtt gaatgccggg 60gagaaacctg tccaatttta gcaggtttga agggaggatc ttcttcagtt gtagtttgga 120aggttccttg gtgtggctca tgaaatcaca gagctcagag ataccatctt gagaaatcct 180ccttggtatc atgaaactgg agcagaggaa ttgcaattta gcaggaggtc ctctactggt 240gataccctca ccttggggta atggtcctaa cccagaccca gggtctggaa gcttaatgtt 300gagttggtga ctccagcctc tttctcctgg aggtcacaag atgatgattg cgtagatgtt 360gcctggtgca aagtgcccca aacagcaata gaaaggcata tgtataacca aactccaagt 420gataaccaga cccatctctc ctccaccttg acaaaagcag attatagtat acaaggtagg 480aattcctgtc ctatttgaga tgaactatat cctgtacctc tgtgctctgt gtctgcatga 540aggctcagcc tttagaggca ctccttctag ttgcattagt actgtctttc tgtggagttt 600ggtttgaaga ctggctcagc aagtggaggt ttcaatgtat ttttcagttg gctcatcagc 660cagcattggt gaatattcag tttaggggaa cagttctagg gagtgagaca tttttgggag 720cagaggaaaa ctctgctgat gttcggtcct ggcaaacatt gagttatttt gagctgtgaa 780ggcagtcgtc tctgttacac agtggcagct cttgagttat gcactgtgaa gaatgagaag 840ggaaaagcaa aaattatcct tgtgaaatat ctgctgattg tgccctactc tttgcacctg 900acttttccta gttgtcctgg tgctaacaca ggagctacac cttgatcctc tcctggcatg 960aaaataaaac aaaggttttc gttgttgttg ttccattgcc catttccccc atgttgtctt 1020tcccttggct gatgcctcct ctgggtcaca ttgcttctta tcctgaacac ttgacacctt 1080gagggtagaa tttagcgttt ggtttttacc tcctagcata tgctgtttgg tatgtgaggg 1140tttcagtaca aatgctgctg tctatttctg tgcacttaac aatggaaccc aaacagaaga 1200gaataaagcc ttgataccaa aattgggaaa gaacatgtgt ccatttggac caaacgttgt 1260tggtttttaa aaaattttat tttgtttttt tgtttttgtt tttgtttttt ttcatcttaa 1320tatgtaccag tggcacttaa ccaaaagata cagtgatata gccatgtact gtgggtggga 1380cagatacagt ctccttggcc tataatgaaa ccactaggac tttatacagt tttccttaat 1440ttgttgacat ataaatggta aattatattt aggcttatcc tgttttgaaa tgatggtagt 1500catctttctt actgctactt tcatgttgct ttctagaaaa cagcatttca ttccaaaata 1560actaggatct gcatttagaa caagaatcat tatttgtcct gaccttttca gtcctacaga 1620gacgcatctg tggttctttt gtacttgcca tagatgtaac ctaaaaagtt ttggcatatt 1680taggtcagcc tagcggaact ttttttttca tttaaatgga gctgaataat ggagattttg 1740tgtctgcaaa attcctgaga tcattgaaaa agtaacaagc tgttccttgt ttctgataca 1800taaaattatt ttaagcattt tatcaatcat taaaatttac tgccagttgt gagtggcttt 1860ttaattaact tgtctttcat tgcacttcac tctgcctgtt ttcaagggga gtaagattgg 1920taacatttgg ggagactgta tctgtctact tagcgtggct gttttgaggg actgtcccat 1980cagtgaacaa actgcatggc cttggagaga gactctgggc tcttggctca gatgtgttca 2040tcaaatactc ctttcagagc tgttgtgggt gtaagtgaca tgatgtggcc aaaaatccaa 2100actgtgcagt tgcgttgtga caaacatgca atgtgctgta aaaattcaat acagtttaaa 2160taaaatctct atattagtgc tgaaaaaaaa aa 2192154913DNAHomo sapiensmisc_featureIncyte Clone No 1738735 154ctcgagcggc tcgagagcgg ggcaaactgc ttggcacctc ttcaataggt gacattcaat 60gatagatctc tggcttcctg ctctgtttgt tctggttgcc ctggaaagcc tgctgctcag 120cccatgcccc gggacttcct ccaccctcac caggacattc tttccatctc ttgtctcctg 180tgtgcaagtc cctttctcct ggattccatg tcttgaatgt ttcttaattt acttcctcat 240tttggcagag gatgtcctcc agttgttttc tgggaatgct aatatgcaag tgaaccagtg 300acctgcagtt ctgcccacac agggttaata accaatcaga ttctctcttt tcaagatggt 360taacataaca gacaccaaga aagggaagag gagccgacag cagaggggga agctgaaaag 420acgcacaaag aatggccata aaagatatga gcaaccccag ctttccagac agtcactttt 480cccagtggtc atacctggtc tggaagattc cccatcatct cgaataaagc tgttgttgct 540tttaactcca tggagagacc gaatggagtg agcccagcag ggcatgctgg gcaagagagg 600tcccccgagt cccaaataag aatttcaact agtataaaac gaggcagcga acccacacgt 660ggaagtctga taccgcttgc agaagggaat tgaatagatg tctccctatt ggtaaggatg 720tggttttatt gacttgaaat aacaaagccc gcaagcaaca actgatcatc cgcgggatgc 780tgccacaagg aataattgag cactcattca gacacagggg aaaccactgc ctctttcagt 840ctttctccca gattccaaca gtcagtgtta cagcatttca ccttgttcac ctccctgaga 900agacgttgca ggg 913155480DNAHomo sapiensmisc_featureIncyte Clone No 1749147 155cttctgttca ggctgggatt acaggtgtga gccgctgcgc tcggccttct ttgattttat 60attattagga gcaaaagtaa atgaagccca ggaaaacacc tttgggaaca aactcttcct 120ttgatggaaa atgcagaggc ccttcctctc tgtgccgtgc ttgctcctct tacctgcccg 180ggtggtttgg gggtgttggt gtttcctccc tggagaagat gggggaggct gtcccactcc 240cagctctggc agaatcaagc

tgttgcagca gtgccttctt catccttcct tacgatcaat 300cacagtctcc agaagatcag ctcaattgct gtgcaggtta aaactacaga accacatccc 360aaaggtacct ggtaagaatg tttgaaagat cttccatttc taggaacccc agtcctgctt 420ctccgcaatg gcacatgctt ccactccatc catactgcag tcgtcaaata aacagatatg 480156545DNAHomo sapiensmisc_featureIncyte Clone No 1817722 156caggctatta agaaaggcgg acccatgcac atgattttaa aggttctgac aactgcattg 60ctgttacaag ctgcttcagc tttagctaat tacattcatt tctccagtta ctccaaagat 120ggaatagggg taccatttat gggaagtttg gcagaatttt ttgacatcgc ttcccaaatt 180cagatgttat acttactttt gagtctatgc atgggttgga caatagtcag aatgaagaag 240tctcaaagca gacctctcca gtgggattct acacctgcat ccactggcat tgcagtattc 300attgtcatga cacagagtgt tttgctactt tgggaacagt ttgaagatat cagtcatcat 360agctaccatt cacaccacaa cttagcaggg atcctcctaa ttgttctaag aatttgccta 420gcattgtcat taggctgtgg actctatcag atcatcacag tggagagaag tacactcaaa 480agggagttct acatcacatt tgccaaagta tgggtttgga aagaaaatgg tttattctga 540ttatc 5451571746DNAHomo sapiensmisc_featureIncyte Clone No 1831290 157ttggcatcag cttgggcagg tgtgcgggct caggatgggg cggccgtggt gaggaaccct 60ggactctcag catcacaaga ggcaacacca ggagccaaca tgagctcggg gactgaactg 120ctgtggcccg gagcagcgct gctggtgctg ttgggggtgg cagccagtct gtgtgtgcgc 180tgctcacgcc caggtgcaaa gaggtcagag aaaatctacc agcagagaag tctgcgtgag 240gaccaacaga gctttacggg gtcccggacc tactccttgg tcgggcaggc atggccagga 300cccctggcgg acatggcacc cacaaggaag gacaagctgt tgcaattcta ccccagcctg 360gaggatccag catcttccag gtaccagaac ttcagcaaag gaagcagaca cgggtcggag 420gaagcctaca tagaccccat tgccatggag tattacaact gggggcggtt ctcgaagccc 480ccagaagatg atgatgccaa ttcctacgag aatgtgctca tttgcaagca gaaaaccaca 540gagacaggtg cccagcagga gggcataggt ggcctctgca gaggggacct cagcctgtca 600ctggccctga agactggccc cacttctggt ctctgtccct ctgcctcccc ggaagaagat 660gaggaatctg aggattatca gaactcagca tccatccatc agtggcgcga gtccaggaag 720gtcatggggc aactccagag agaagcatcc cctggcccgg tgggaagccc agacgaggag 780gacggggaac cggattacgt gaatggggag gtggcagcca cagaagccta gggcagacca 840agaagaaagg agccaaggca aagagggacc actgtgctca tggacccatc gctgccttcc 900aaggaccatt tcccagagct actcaacttt taagcccctg ccatggttgc tcctggaagg 960agaaccagcc accctgagga ccacctggcc atgcgtgcac agcctgggaa aagacagtta 1020ctcacgggag ctgcaggccc gtcaccaagc cctctcccga cccaggcttt gtggggcagg 1080cacctggtac caagggtaac ccggctcctg gtatggacgg atgcgcagga tttaggataa 1140gctgtcaccc agtccccata acaaaaccac tgtccaacac tggtatctgt gttcttttgt 1200gctatgaatt tggattccta attgctattg ttggttgctg gggttttaaa tgattgataa 1260gcttgtacag ttaacttata gagggggagc catatttaac attctggatt tcagagtaga 1320gatttctgtg ttgtctccta gaaagcatta catgtagttt atttcagcat ccttgttggg 1380tggggccctg gctctcttcc cctttggtgg gacctcccct ttctttgggc ttcagttcac 1440tcaggaagaa atgaggctgt cgccatcttt atgtgcttcc agtggaaatg tcacttgcta 1500cagacaatag tgcatgagag tctagagaag tagtgaccag aacagggcag agtaggtccc 1560ctccatggcc ctgaatcctc ctctgctcca gggctggcct ctgcagagct gattaaacag 1620tgttgtgact gtctcatggg aagagctggg gcccagaggg accttgagtc agaaatgttg 1680ccagaaaaag tatctcctcc aaccaaaaca tctcaataaa accattttag ttgaaaaaaa 1740aaaaaa 17461582011DNAHomo sapiensmisc_featureIncyte Clone No 1831477 158ggagcacggc gctggggccg cccgcagcgc tcactcgctc gcactcagtc gcgggaggct 60tccccgcgcc ggccgcgtcc cgcccgctcc ccggcaccag aagttcctct gcgcgtccga 120cggcgacatg ggcgtcccca cggccccgga ggccggcagc tggcgctggg gatccctgct 180cttcgctctc ttcctggctg cgtccctagg tccggtggca gccttcaagg tcgccacgcc 240gtattccctg tatgtctgtc ccgaggggca gaacgtcacc ctcacctgca ggctcttggg 300ccctgtggac aaagggcacg atgtgacctt ctacaagacg tggtaccgca gctcgagggg 360cgaggtgcag acctgctcag agcgccggcc catccgcaac ctcacgttcc aggaccttca 420cctgcaccat ggaggccacc aggctgccaa caccagccac gacctggctc agcgccacgg 480gctggagtcg gcctccgacc accatggcaa cttctccatc accatgcgca acctgaccct 540gctggatagc ggcctctact gctgcctggt ggtggagatc aggcaccacc actcggagca 600cagggtccat ggtgccatgg agctgcaggt gcagacaggc aaagatgcac catccaactg 660tgtggtgtac ccatcctcct cccaggagag tgaaaacatc acggctgcag ccctggctac 720gggtgcctgc atcgtaggaa tcctctgcct ccccctcatc ctgctcctgg tctacaagca 780aaggcaggca gcctccaacc gccgtgccca ggagctggtg cggatggaca gcaacattca 840agggattgaa aaccccggct ttgaagcctc accacctgcc caggggatac ccgaggccaa 900agtcaggcac cccctgtcct atgtggccca gcggcagcct tctgagtctg ggcggcatct 960gctttcggag cccagcaccc ccctgtctcc tccaggcccc ggagacgtct tcttcccatc 1020cctggaccct gtccctgact ctccaaactt tgaggtcatc tagcccagct gggggacagt 1080gggctgttgt ggctgggtct ggggcaggtg catttgagcc agggctggct ctgtgagtgg 1140cctccttggc ctcggccctg gttccctccc tcctgctctg ggctcagata ctgtgacatc 1200ccagaagccc agcccctcaa cccctctgga tgctacatgg ggatgctgga cggctcagcc 1260cctgttccaa ggattttggg gtgctgagat tctcccctag agacctgaaa ttcaccagct 1320acagatgcca aatgacttac atcttaagaa gtctcagaac gtccagccct tcagcagctc 1380tcgttctgag acatgagcct tgggatgtgg cagcatcagt gggacaagat ggacactggg 1440ccaccctccc aggcaccaga cacagggcac ggtggagaga cttctccccc gtggccgcct 1500tggctccccc gttttgcccg aggctgctct tctgtcagac ttcctctttg taccacagtg 1560gctctggggc caggcctgcc tgcccactgg ccatcgccac cttccccagc tgcctcctac 1620cagcagtttc tctgaagatc tgtcaacagg ttaagtcaat ctggggcttc cactgcctgc 1680attccagtcc ccagagcttg gtggtcccga aacgggaagt acatattggg gcatggtggc 1740ctccgtgagc aaatggtgtc ttgggcaatc tgaggccagg acagatgttg ccccacccac 1800tggagatggt gctgagggag gtgggtgggg ccttctggga aggtgagtgg agaggggcac 1860ctgccccccg ccctccccat cccctactcc cactgctcag cgcgggccat tgcaagggtg 1920ccacacaatg tcttgtccac cctgggacac ttctgagtat gaagcgggat gctattaaaa 1980actacatggg gaaacaggtg caaaaaaaaa a 2011159480DNAHomo sapiensmodified_base(440)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 1841607 159cccacgcgtc cgaaaagaaa agaaaaaaga aaatggcctc atcttgtttc agcctcagtt 60ttcctcccct cagtctggct gggagcttag ctctttgggg tcattgctgt gtcaggctgg 120gttgttcctt ttggtctgtt tctgccatgg cccagcgcct tccctctcag aatacataca 180atccccccct ctgctgggcg tggtgactca tgtctataat cccagctctt tgggaggcca 240gggcgggtgg atcacttgag cctaggagtt cgaaaccagc ctgagcaaca tggtgaaagc 300ccatctctac gaaaaatgca aaagttagcc aggcatggtg gtgcacgtct gtagacccag 360ctacttggga ggctgaggca ggaggatcct tgagcccagg aggcagaagc tgcagtgagc 420tgtgatcgca ccactgctcn tcagcctggc gacagagcga gattccctca aaaaaaaaaa 480160542DNAHomo sapiensmisc_featureIncyte Clone No 1852391 160ttgaatcaac ataatgtatg tgaaagggct tagtccatga ttgggtgctt aatatgcccg 60tgttgctggg gtgagccaaa gggatgaagt tggcagtgct tgctctgtcg tggagcagtc 120cccacgtggg aaggccagcg ggaaaccagg cctgctgaag tctccagcgc tggaagcctc 180acgggggtta ggaaggagcc ttgggagcag ctcctcagag cacagttgta cctcaattgt 240ggattttaga tgtttctgct tctcaatgtt ctctcttttt tcctgcctgc ttgcctgcct 300tttggacctc ttgctgtcta gggtggcaga tgaagctttc tacaaacaac ccttcgctga 360cgtgattggt tatgtgtatg ttgcaaaact aattcctttt tctacatctg attctttcta 420cttttgttta gagttaatgc tccttttatg tcaccagttg ctttgctttt taaattattt 480caaattggca ctttgggggc tgcctaagaa ttgataagcg gggtatgatc tgttgatgaa 540tc 5421611066DNAHomo sapiensmisc_featureIncyte Clone No 1854555 161ttaggctttc tgtatttgtc tgaatgcttt cacgggagtg tgtcgcactg gagcacagag 60gacactcgat cgtgcggcgc gcagggcggg gggccgccgc tgcctccccg cgggatggct 120ggcactgtgc tcggagtcgg tgcgggcgtg ttcatcttag ccctgctctg ggtggcagtg 180ctgctgctgt gtgtgctgct gtccagagcc tccggggcgg cgaggttctc tgtcattttt 240ttattcttcg gtgctgtgat catcacatca gttctgttgc ttttcccgcg agctggtgaa 300ttcccagccc cagaagtgga agttaagatt gtggatgact ttttcattgg ccgctatgtc 360ctgctggctt tccttagtgc catcttcctt ggaggcctct tcttggtttt aatccattat 420gttctggagc cgatctatgc caaaccactg cactcctact gaccactctt caggaaaacg 480aaaacctgtt ctctccttca ttgtgatgac attgatgagc aggaaggcac tattcagagc 540cttgttttga cagccctcat gccttaaggt tagaggagta tctgtccatc actaagacaa 600atctctggag tcctggcttc cagaaacagg attgccaaat tgtccctgtg gggctagatt 660cttaccagct taagaaggat attgctatct tcttagtacc cgtaccttag gatttccaac 720tgttttgaaa gggaaatagt aacagtgatc tgcttagagt ggattttcac tcaagtcctt 780agtaagtgga ttttggggaa aaaagcacat gggcttctgg ttctttttga taatatataa 840aattattcat tatgaggttg cagttgtttg caaaggagag gcactcaaat ttgaaaggtt 900attttaatgt gataatttgg aagacttact cagatgttgg tcattgacca ctctgtgcat 960atatttctgc agagctctgt gaaggcaatg agtgtcactt ccctctgctc taataaagca 1020ataaataata gctaaagggc tgactttcac ttcgaaaaaa aaaaaa 10661621173DNAHomo sapiensmisc_featureIncyte Clone No 1855755 162gtctcgctcc tgcccagccc gggcggctgc ccttgggtgc tcccttccct gcccgacacc 60cagaccgacc ttgaccgccc acctggcagg agcaggacag gacggccgga cgcggccatg 120gccgagctcc cggggccctt tctctgcggg gccctgctag gcttcctgtg cctgagtggg 180ctggccgtgg aggtgaaggt acccacagag ccgctgagca cgcccctggg gaagacagcc 240gagctgacct gcacctacag cacgtcggtg ggagacagct tcgccctgga gtggagcttt 300gtgcagcctg ggaaacccat ctctgagtcc catccaatcc tgtacttcac caatggccat 360ctgtatccaa ctggttctaa gtcaaagcgg gtcagcctgc ttcagaaccc ccccacagtg 420ggggtggcca cactgaaact gactgacgtc cacccctcag atactggaac ctacctctgc 480caagtcaaca acccaccaga tttctacacc aatgggttgg ggctaatcaa ccttactgtg 540ctggttcccc ccagtaatcc cttatgcagt cagagtggac aaacctctgt gggaggctct 600actgcactga gatgcagctc ttccgagggg gctcctaagc cagtgtacaa ctgggtgcgt 660cttggaactt ttcctacacc ttctcctggc agcatggttc aagatgaggt gtctggccag 720ctcattctca ccaacctctc cctgacctcc tcgggcacct accgctgtgt ggccaccaac 780cagatgggca gtgcatcctg tgagctgacc ctctctgtga ccgaaccctc ccaaggccga 840gtggccggag ctctgattgg ggtgctcctg ggcgtgctgt tgctgtcagt tgctgcgttc 900tgcctggtca ggttccagaa agagaggggg aagaagccca aggagacata tgggggtagt 960gaccttcggg aggatgccat cgctcctggg atctctgagc acacttgtat gagggctgat 1020tctagcaagg ggttcctgga aagaccctcg tctgccagca ccgtgacgac caccaagtcc 1080aagctcccta tggtcgtgtg acttctcccg atccctgagg gcggtgaggg ggaatatcaa 1140taattaaagt ctgtgggtac caaaaaaaaa aaa 1173163890DNAHomo sapiensmisc_featureIncyte Clone No 1861434 163ctcgagccgg agagatcctc taccgcagtc gtttgaggag gcggaactga agttttttct 60taattatcat gtgacgggtt ctggatttaa tggggggaaa agggcggaaa aggacaagga 120tccaaactgg cgaatttgct gatcttcgcg tccctctccg ctttccggcc ggcagcgctg 180ccagggtata tttccttttt tccgatcctg caacagcctc tttaaactgt ttaaatgaga 240atgtccttgg ctcagagagt actactcacc tggcttttca cactactctt cttgatcatg 300ttggtgttga aactggatga gaaagcacct tggaactggt tcctcatatt cattccagtc 360tggatatttg atactatcct tcttgtcctg ctgattgtga aaatggctgg gcggtgtaag 420tctggctttg accctcgaca tggatcacac aatattaaaa aaaaagcctg gtacctcatt 480gcaatgttac ttaaattagc cttctgcctc gcactctgtg ctaaactgga acagtttact 540accatgaatc tatcctatgt cttcattcct ttatgggcct tgctggctgg ggctttaaca 600gaactcggat ataatgtctt ttttgtgaga gactgacttc taagtacatc atctcctttc 660tattgctgtt caacaagtta ccattaaagt gttctgaatc tgtcaagctt caagaatacc 720agagaactga gggaaaatac caaatgtagt tttatactac ttccataaaa caggattggt 780gaatcacgga cttctagtca acctacagct taattattca gcatttgagt tattgagatc 840cttattatct ctatgtaaat aaagtttgtt ttggacctca aaaaaaaaaa 890164806DNAHomo sapiensmisc_featureIncyte Clone No 1872334 164tgcatcagtg cccaggcaag cccaggagtt gacatttctc tgcccagcca tgggcctcac 60cctgctcttg ctgctgctcc tgggactaga aggtcagggc atagttggca gcctccctga 120ggtgctgcag gcacccgtgg gaagctccat tctggtgcag tgccactaca ggctccagga 180tgtcaaagct cagaaggtgt ggtgccggtt cttgccggag gggtgccagc ccctggtgtc 240ctcagctgtg gatcgcagag ctccagcggg caggcgtacg tttctcacag acctgggtgg 300gggcctgctg caggtggaaa tggttaccct gcaggaagag gatgctggcg agtatggctg 360catggtggat ggggccaggg ggccccagat tttgcacaga gtctctctga acatactgcc 420cccaggtgag ttatcctagg ccagctacca ccccttagac ctaccctccc cacccccgcc 480tattgccagg gctcatgggt tcttgaggag tgggggcccc tggggaggag gcattccaag 540gagatatcct cttgacagct ctgcagggag cggaaaccaa actgggtggg aagtctgaga 600taaatcagct gaaaaccatc cctttccccc ttccacacta ctgcgcttcc ccacaggaag 660gcatgtcctt cccactccag ggacttggcc tcttcttcca gcattttcaa catacttgat 720gctaacttat tttttaatta gaaatatttt aaacaatgtt gaatctgagt gtataaaaca 780gaataatttt tgtagctcca gtgttt 8061651923DNAHomo sapiensmisc_featureIncyte Clone No 1877230 165tggccggcaa gcagggctgc agtcacgggg cggcgcggag ggccccagcc cagtcagggg 60tgtggccgcc gccaccgtaa ggctaggccg cgagcttagt cctgggagcc gcctccgtcg 120ccgccgtcag agccgcccta tcagattatc ttaacaagaa aaccaactgg aaaaaaaaat 180gaaattcctt atcttcgcat ttttcggtgg tgttcacctt ttatccctgt gctctgggaa 240agctatatgc aagaatggca tctctaagag gacttttgaa gaaataaaag aagaaatagc 300cagctgtgga gatgttgcta aagcaatcat caacctagct gtttatggta aagcccagaa 360cagatcctat gagcgattgg cacttctggt tgatactgtt ggacccagac tgagtggctc 420caagaaccta gaaaaagcca tccaaattat gtaccaaaac ctgcagcaag atgggctgga 480gaaagttcac ctggagccag tgagaatacc ccactgggag aggggagaag aatcagctgt 540gatgctggag ccaagaattc ataagatagc catcctgggt cttggcagca gcattgggac 600tcctccagaa ggcattacag cagaagttct ggtggtgacc tctttcgatg aactgcagag 660aagggcctca gaagcaagag ggaagattgt tgtttataac caaccttaca tcaactactc 720aaggacggtg caataccgaa cgcagggggc ggtggaagct gccaaggttg gggctttggc 780atctctcatt cgatccgtgg cctccttctc catctacagt cctcacacag gtattcagga 840ataccaggat ggcgtgccca agattccaac agcctgtatt acggtggaag atgcagaaat 900gatgtcaaga atggcttctc atgggatcaa aattgtcatt cagctaaaga tgggggcaaa 960gacctaccca gatactgatt ccttcaacac tgtagcagag atcactggga gcaaatatcc 1020agaacaggtt gtactggtca gtggacatct ggacagctgg gatgttgggc agggtgccat 1080ggatgatggc ggtggagcct ttatatcatg ggaagcactc tcacttatta aagatcttgg 1140gctgcgtcca aagaggactc tgcggctggt gctctggact gcagaagaac aaggtggagt 1200tggtgccttc cagtattatc agttacacaa ggtaaatatt tccaactaca gtctggtgat 1260ggagtctgac gcaggaacct tcttacccac tgggctgcaa ttcactggca gtgaaaaggc 1320cagggccatc atggaggagg ttatgagcct gctgcagccc ctcaatatca ctcaggtcct 1380gagccatgga gaagggacag acatcaactt ttggatccaa gctggagtgc ctggagccag 1440tctacttgat gacttataca agtatttctt cttccatcac tcccacggag acaccatgac 1500tgtcatggat ccaaagcaga tgaatgttgc tgctgctgtt tgggctgttg tttcttatgt 1560tgttgcagac atggaagaaa tgctgcctag gtcctagaaa cagtaagaaa gaaacgtttt 1620catgcttctg gccaggaatc ctgggtctgc aactttggaa aactcctctt cacataacaa 1680tttcatccaa ttcatcttca aagcacaact ctatttcatg ctttctgtta ttatctttct 1740tgatactttc caaattctct gattctagaa aaaggaatca ttctcccctc cctcccacca 1800catagaatca acatatggta gggattacag tgggggcatt tctttatatc acctcttaaa 1860aacattgttt ccactttaaa agtaaacact taataaattt ttggaagatc aaaaaaaaaa 1920aaa 1923166518DNAHomo sapiensmisc_featureIncyte Clone No 1877885 166ttgacaccag cagggtgaca tccgctattg ctacttctct gctcccccac agttcctctg 60gacttctctg gaccacagtc ctctgccaga cccctgccag accccagtcc accatgatcc 120atctgggtca catcctcttc ctgcttttgc tcccagtggc tgcagctcag acgactccag 180gagagagatc atcactccct gccttttacc ctggcacttc aggctcttgt tccggatgtg 240ggtccctctc tctgccgctc ctggcaggcc tcgtggctgc tgatgcggtg gcatcgctgc 300tcatcgtggg ggcggtgttc ctgtgcgcac gcccacgccg cagccccgcc caagaagatg 360gcaaagtcta catcaacatg ccaggcaggg gctgaccctc ctgcagcttg gacctttgac 420ttctgaccct ctcatcctgg atggtgtgtg gtggcacagg aacccccgcc ccaacttttg 480gattgtaata aaacaattga aacaccaaaa aaaaaaaa 5181671631DNAHomo sapiensmisc_featureIncyte Clone No 1889269 167gcgagctctc agcgggagcc gagacggtgc agggccggag aagcaccttc actcccagcc 60tgcgccccga tgctgcgcgt tctgtgcctc ctgcgcccct ggaggcccct tcgggcccgc 120ggctgcgctt ccgacggggc ggccgggggc tcagagatcc aagtgcgcgc cctggcgggt 180ccggaccaag gctgtaggtt ccatgaggac aggccttgag tctgtcctgg tctctggaat 240cacggtgtct agtagaggcc agcacacagc aaatatataa atgtacaaat gagtgaatga 300agagaatctg attggcctta aggaacttac gcacttaaaa taattgggca gaagagaagc 360agtgaaggag tgcagaggca tcacctgaaa gtttacaagt ccttccactt tctctctgag 420gcagaaagag caagggtttt tctctccatt ttatggttgg gaaaattgag gcctgcctga 480gtgtgtgact tgtggcaagt cactctggtc atctagggca gaggctcccc agatcccagg 540cctcctgcct ccagtcccca gcccgcagcc caggattagg cagagccagc tgctttcccg 600tggctgccct gactccttac agggatcact gagattctga tgaacagacc ttctgcccgc 660aatgccttgg ggaatgtctt cgtcagtgag ctgctggaaa ctctggccca gctgcgggag 720gaccggcaag tgcgtgtcct gctcttcaga agtggagtga agggcgtgtt ctgtgcaggt 780gcagacctga aggagcggga acagatgagt gaagcagagg tgggggtgtt tgtccagcga 840ctccggggcc tgatgaatga catcggtgag gatctgggtg tagggtggag gagggggttt 900gggggtccct gccgatgaca gtcccgctac ccccaccagc atctaaggag agtcttcttt 960ctgtttggag ttctgtgata agacagatga ctcacccagg gggatggagg aggatgaccg 1020agggcagttc tctcagagag ggagttctgg ctcttcagct tttgtgtccc gccccaccct 1080cagggttcaa gcctggccat tccaaagcag ttaagtttcc ccaagcatgc tttcaagttt 1140tgacaattgc tgttaccttt gcctgagata ccccttcttg gttacttgaa cttttacttg 1200tccttcaagc cctccagtac ctcctcctcc aggaagcctt cccaacccac cctctgagct 1260ttttattgga gcactgatga tcctgggtca ataatgcctg atacacattt gtcttcccca 1320tgagactgag ccccatggga acaaaggcta tgtctgattc attctgtgtt cccagttccc 1380agcacccagc acagggcttg gcacaaagaa agggaggccc cagggaggcc agcggattag 1440gcctgaacag ggatcatcca gcccatcctc ccattcctct tccctggctg attctgtaac 1500tttccctaaa gggaaaattg gcttctgaga taacctggct gcgggaagca gaggttgtcg 1560tgagcagaga ttgtgccatt gcactccagc ctgggcaaca acagcgagac tccatctcaa 1620aaaaaaaaaa a 16311681548DNAHomo sapiensmisc_featureIncyte Clone No 1890243 168atgcgctcca gcagcctgtt tgggaagcag cagtctctcc ttcagatact gtgggactca 60tgctggagag gagccgccca cttccaggac ctgtgaataa gggctaatga tgagggttgg 120tggggctctc tgtggggcaa aaaggtggta tgggggttag cactggctct cgttctcacc 180ggagaaggaa gtgttctagt gtggtttagg aaacatgtgg ataaagggaa ccatgaaaat 240gagaggagga aagacatcca gatcagctgt tttgcctgtt gctcagttga ctctgattgc

300atcctgtttt cctaattccc agactgttct gggcacggaa gggaccctgg atgtggagtc 360ttcccctttg gccctcctca ctggcctctg ggctagccca gagtccctta gcttgtacct 420cgtaacactc ctgtgtgtct gtccagcctt gcagtcatgt caaggccagc aagctgatgt 480gactcttgcc ccatgcgaga tatttatacc tcaaacactg gcctgtgagc cctttccaag 540tcagtggaga gccctgaaag gagcctcact tgaatccagc tcagtgctct gggtggcccc 600ctgcaggtgg cccctgaccc tgcgttgcag cagggtccac ctgtgagcag gcccgccctg 660gggcctcttc ctggatgtgc cctctctgag ttctgtgctg tctcttggag gcagggccca 720ggagaacaaa gtgtggaggc ctcggggagt ggcttttcca gctctcatgc cccgcagtgt 780ggaacaaggc agaaaaggat cctaggaaat aagtctcttg gcggtccctg agagtcctgc 840tgaaatccag ccagtgtttt ttgtggtatg agaacaggca aaaagagatg ccccgagata 900gaaggggagc cttgtgtttc tttcctgcag acgtgagatg aacactggag tgggcagagg 960tggcccagga ccatggcacc cttagagtgc agaagctggg gggagaggct gcttcgaagg 1020gcaggactgg ggataatcag aacctgcctg tcacctcagg gcatcactga acaaacattt 1080cctgatggga actcctgcgg cagagcccag gctggggaag tgaactaccc agggcagccc 1140ctttgtggcc caggataatc aacactgttc tctctgtacc atgagctcct ccaggagatt 1200atttaagtgt attgtatcat tggttttctg tgattgtcat aacattgttt ttgttattgt 1260tggtgctgtt gttatttatt attgtaattt cagtttgcct ctactggaga atctcagcag 1320gggtttcagc ctgactgtct ccctttctct accagactct acctctgaat gtgctgggaa 1380cctcttggag cctgtcagga actcctcact gtttaaatat ttatttattg tgacaaatgg 1440agctggtttc ctagatatga atgatgtttg caatccccat tttcctgttt cagcatgtta 1500tattcttata aaataaaagc aaaagtcaaa tatgaaaaaa aaaaaaaa 1548169616DNAHomo sapiensmisc_featureIncyte Clone No 1900433 169gccagctcag gtgagccctc gccaaggtga cctcgcagga cactggtgaa ggagcagtga 60ggaacctgca gagtcacaca gttgctgacc aattgagctg tgagcctgga gcagatccgt 120gggctgcaga cccccgcccc agtgcctctc cccctgcagc cctgcccctc gaactgtgac 180atggagagag tgaccctggc ccttctccta ctggcaggcc tgactgcctt ggaagccaat 240gacccatttg ccaataaaga cgatcccttc tactatgact ggaaaaacct gcagctgagc 300ggactgatct gcggagggct cctggccatt gctgggatcg cggcagttct gagtggcaaa 360tgcaaataca agagcagcca gaagcagcac agtcctgtac ctgagaaggc catcccactc 420atcactccag gctctgccac tacttgctga gcacaggact ggcctccagg gatggcctga 480agcctaacac tggcccccag cacctcctcc cctgggaggc cttatcctca aggaaggact 540tctctccaag ggcaggctgt taggcccctt tctgatcagg aggcttcttt atgaattaaa 600ctcgccccac cacccc 6161701981DNAHomo sapiensmisc_featureIncyte Clone No 1909441 170cagaacttct ttttgacacc atagattctt ctgaggtcaa cgttgcaaaa agcatagcaa 60agtttcttcg aaatgttaga tatcgttatc aaccactatt agaaagatgt aataacgtat 120ttttaagtaa tgtggaccac cttgatttgg attccatcag taaaatactt agtgtataca 180aatttctaca atttaatagt tttgaattta ttataatggc taaaaagaag ctaactgaaa 240tgattcctct gtgtaatcat cctgctagct ttgtaaaatt gtttgtagca ttgggaccca 300ttgcaggacc tgaagaaaag aaacaactta aatcaactat gttattgatg tcagaggacc 360taactggcga gcaagccctg gcagtgttgg gagcaatggg agatatggaa agcagaaact 420catgtctgat taaaagagtt acttcagttc tgcataaaca tttggatggc tataaaccat 480tagagttgtt gaagataact caagaattga cttttctgca tttccaaagg aaggagtttt 540ttgcgaaact tagagaatta ctgcttagtt atttgaaaaa tagtttcata ccaactgagg 600tgtctgttct ggtccgtgct atttccctgc tcccttctcc tcacttggac gaagtgggga 660tatcccgaat tgaagccgtt ttaccacagt gtgacctaaa taacctgagt agttttgcca 720catctgtttt aagatggatt cagcatgatc acatgtattt ggataatatg actgcgaaac 780aactgaaact acttcaaaaa ttagatcact atggtcgtca gagactacaa cacagcaaca 840gtttggatct gttacggaag gaacttaaat ctctcaaagg aaacacgttt cctgagtcac 900ttcttgaaga aatgattgct actttacagc atttcatgga tgatattaat tacataaatg 960ttggggagat tgcatctttt atttctagta ctgattacct cagtactttg ctactagata 1020ggatagcctc agtggctgtt cagcagattg aaaagatcca tccttttaca atccctgcta 1080ttattcgtcc attcagcgta ttgaactatg atccacctca aagggatgaa tttttgggaa 1140cttgcgtgca acatcttaat tcttacttag gtatattgga tccttttata ttagtgtttc 1200ttggtttctc tttggccaca cttgaatatt ttccagaaga tctgctaaag gcaattttta 1260acatcaaatt cttagctaga ttggattctc aacttgaaat tttatctcca tctcgaagtg 1320caagagtcca gtttcatctt atggagttaa atagatcagt ctgcttggaa tgccctgagt 1380ttcagattcc atggtttcat gaccgcttct gtcaacaata taataaaggt attggtggca 1440tggatggaac acaacagcag atttttaaaa tgttagcaga ggtactagga ggaatcaatt 1500gtgtaaaagc ctcggttctt acgccttatt accacaaagt agattttgag tgtatcttgg 1560ataaaagaaa aaaacctctt ccgtatggaa gccataatat agcattggga caactaccag 1620aaatgccctg ggaatcaaat atcgaaatag ttggatcaag gctgccacca ggggctgaaa 1680ggattgcttt ggaatttttg gattcaaaag cactttgtag aaatatccct cacatgaaag 1740gaaaatctgc tatgaaaaaa cgacatttgg aaattctggg gtatcgtgta attcagattt 1800cccagtttga atggaactct atggcactgt caacaaagga tgctcggatg gactacctga 1860gagaatgtat atttggagaa gtcaagtcat gtttgtagtt tttatttaaa atgaatgtta 1920tcgtgtgtta catttggacc tattttaata aagtggcctg tctcaattaa aaaaaaaaaa 1980g 19811711492DNAHomo sapiensmisc_featureIncyte Clone No 1932226 171cttctgggtg aggagtttga gcttggctgg gtccagggcc cagcactgac tcccgtccct 60gaggaggagg aagaagagga agagggggct ccgattggga cccctaggga tcctggagat 120ggttgtcctt cccccgacat ccctcctgaa ccccctccaa cacacctgag gccctgccct 180gccagccagc tccctggact cctgtcccat ggcctcctgg ccggcctctc ctttgcagtg 240gggtcctcct ctggcctcct gcccctcctg ctgctgctgc tgcttccatt gctggcagcc 300cagggtgggg gtggcctgca ggcagcgctg ctggcccttg aggtggggct ggtgggtctg 360ggggcctcct acctgctcct ttgtacagcc ctgcacctgc cctccagtct tttcctactc 420ctggcccagg gtaccgcact gggggccgtc ctgggcctga gctggcgccg aggcctcatg 480ggtgttcccc tgggccttgg agctgcctgg ctcttagctt ggccaggcct agctctacct 540ctggtggcta tggcagcggg gggcagatgg gtgcggcagc agggcccccg ggtgcgccgg 600ggcatatctc gactctggtt gcgggttctg ctgcgcctgt cacccatggc cttccgggcc 660ctgcagggct gtggggctgt gggggaccgg ggtctgtttg cactgtaccc caaaaccaac 720aaggatggct tccgcagccg cctgcccgtc cctgggcccc ggcggcgtaa tccccgcacc 780acccaacacc cattagctct gttggcaagg gtctgggtcc tgtgcaaggg ctggaactgg 840cgtctggcac gggccagcca gggtttagca tcccacttgc ccccgtgggc catccacaca 900ctggccagct ggggcctgct tcggggtgaa cggcccaccc gaatcccccg gctactacca 960cgcagccagc gccagctagg gccccctgcc tcccgccagc cactgccagg gactctagcc 1020gggcggaggt cacgcacccg ccagtcccgg gccctgcccc cctggaggta gctgactcca 1080gcccttccag cccaaatcta gagcattgag cactttatct cccacgactc agtgaagttt 1140ctccagtccc tagtcctctc ttttcaccca ccttcctcag tttgctcact taccccaggc 1200ccagcccttc ggacctctag acaggcagcc tcctcagctg tggagtccag cagtcactct 1260gtgttctcct ggcgctcctc ccctaagtta ttgctgttcg cccgctgtgt gtgctcatcc 1320tcaccctcat tgactcaggc ctggggccag gggtggtgga gggtgggaag agtcatgttt 1380tttttctcct ctttgatttt gtttttctgt ctcccttcca acctgtcccc ttccccccac 1440caaaaaaaga aaaagacaaa cacaaataaa atatctgagc ggaaaaaaaa aa 14921721613DNAHomo sapiensmisc_featureIncyte Clone No 1932647 172ctcggaattc ggctcgagac gggtcatgag cgcggtatta ctgctggccc tcctggggtt 60catcctccca ctgccaggag tgcaggcgct gctctgccag tttgggacag ttcagcatgt 120gtggaaggtg tccgacctac cccggcaatg gacccctaag aacaccagct gcgacagcgg 180cttggggtgc caggacacgt tgatgctcat tgagagcgga ccccaagtga gcctggtgct 240ctccaagggc tgcacggagg ccaaggacca ggagccccgc gtcactgagc accggatggg 300ccccggcctc tccctgatct cctacacctt cgtgtgccgc caggaggact tctgcaacaa 360cctcgttaac tccctcccgc tttgggcccc acagccccca gcagacccag gatccttgag 420gtgcccagtc tgcttgtcta tggaaggctg tctggagggg acaacagaag agatctgccc 480caaggggacc acacactgtt atgatggcct cctcaggctc aggggaggag gcatcttctc 540caatctgaga gtccagggat gcatgcccca gccaggttgc aacctgctca atgggacaca 600ggaaattggg cccgtgggta tgactgagaa ctgcaatagg aaagattttc tgacctgtca 660tcgggggacc accattatga cacacggaaa cttggctcaa gaacccactg attggaccac 720atcgaatacc gagatgtgcg aggtggggca ggtgtgtcag gagacgctgc tgctcataga 780tgtaggactc acatcaaccc tggtggggac aaaaggctgc agcactgttg gggctcaaaa 840ttcccagaag accaccatcc actcagcccc tcctggggtg cttgtggcct cctataccca 900cttctgctcc tcggacctgt gcaatagtgc cagcagcagc agcgttctgc tgaactccct 960ccctcctcaa gctgcccctg tcccaggaga ccggcagtgt cctacctgtg tgcagcccct 1020tggaacctgt tcaagtggct ccccccgaat gacctgcccc aggggcgcca ctcattgtta 1080tgatgggtac attcatctct caggaggtgg gctgtccacc aaaatgagca ttcagggctg 1140cgtggcccaa ccttccagct tcttgttgaa ccacaccaga caaatcggga tcttctctgc 1200gcgtgagaag cgtgatgtgc agcctcctgc ctctcagcat gagggaggtg gggctgaggg 1260cctggagtct ctcacttggg gggtggggct ggcactggcc ccagcgctgt ggtggggagt 1320ggtttgccct tcctgctaac tctattaccc ccacgattct tcaccgctgc tgaccaccca 1380cactcaacct ccctctgacc tcataaccta atggccttgg acaccagatt ctttcccatt 1440ctgtccatga atcatcttcc ccacacacaa tcattcatat ctattcacct aacagcaaca 1500ctggggagag cctggagcat ccggacttgc cctatgggag aggggacgct ggaggagtgg 1560ctgcatgtat ctgataatac agaccctgtc ctttctccca aaaaaaaaaa aaa 16131731622DNAHomo sapiensmisc_featureIncyte Clone No 2124245 173tgtcgcgccc gctggccggc tccgccctca cctcccggcc gcggctgccc tctgcccggg 60ttgtccaaga tggagggcgc tccaccgggg tcgctcgccc tccggctcct gctgttcgtg 120gcgctacccg cctccggctg gctgacgacg ggcgcccccg agccgccgcc gctgtccgga 180gccccacagg acggcatcag aattaatgta actacactga aagatgatgg ggacatatct 240aaacagcagg ttgttcttaa cataacctat gagagtggac aggtgtatgt aaatgactta 300cctgtaaata gtggtgtaac ccgaataagc tgtcagactt tgatagtgaa gaatgaaaat 360cttgaaaatt tggaggaaaa agaatatttt ggaattgtca gtgtaaggat tttagttcat 420gagtggccta tgacatctgg ttccagtttg caactaattg tcattcaaga agaggtagta 480gagattgatg gaaaacaagt tcagcaaaag gatgtcactg aaattgatat tttagttaag 540aaccggggag tactcagaca ttcaaactat accctccctt tggaagaaag catgctctac 600tctatttctc gagacagtga cattttattt acccttccta acctctccaa aaaagaaagt 660gttagttcac tgcaaaccac tagccagtat cttatcagga atgtggaaac cactgtagat 720gaagatgttt tacctggcaa gttacctgaa actcctctca gagcagagcc gccatcttca 780tataaggtaa tgtgtcagtg gatggaaaag tttagaaaag atctgtgtag gttctggagc 840aacgttttcc cagtattctt tcagtttttg aacatcatgg tggttggaat tacaggagca 900gctgtggtaa taaccatctt aaaggtgttt ttcccagttt ctgaatacaa aggaattctt 960cagttggata aagtggacgt catacctgtg acagctatca acttatatcc agatggtcca 1020gagaaaagag ctgaaaacct tgaagataaa acatgtattt aaaacgccat ctcatatcat 1080ggactccgaa gtagcctgtt gcctccaaat ttgccacttg aatataattt tctttaaatc 1140gttaagaatc agtttataca ctagagaaat tgctaaactc taagactgcc tgaaaattga 1200cctttacagt gccaagttaa agtttacctt attctcggcc gggtgcagtg gctcatgcct 1260gtaatcccag gactttggga ggccaatgcg ggcggatcac gaggtcagat caagaccatc 1320ctgccaacat ggtgaaaccc tgtctctact aaaaaaaata aaaaaattag ctgggtgtgg 1380cggtgcacgc ctgtagtccc agctacttgg gaggctgagg caggagaatt gcttgaaccc 1440gggaggcgga ggctgcagtg agccaggatc acgccactgc actccagcct gggtgacaga 1500gcgagactct gtttcaaaaa aaaaaaagtt gaccttattc tctaaaaggg ctggctattc 1560atatgatgaa ttgttaagga aaacttaaag tggacaagaa caggatgtga agagaggtga 1620tg 16221741320DNAHomo sapiensmisc_featureIncyte Clone No 2132626 174gcgtgaccca gctgcggccg gccagccatg gagactggag cgctgcggcg cccgcaactt 60ctcccgttgc tgctgctgct ctgcggtggg tgtcccagag caggcggctg caacgagaca 120ggcatgttgg agaggctgcc cctgtgtggg aaggctttcg cagacatgat gggcaaggtg 180gacgtctgga agtggtgcaa cctgtccgag ttcatcgtgt actatgagag tttcaccaac 240tgcaccgaga tggaggccaa tgtcgtgggc tgctactggc ccaaccccct ggcccagggc 300ttcatcaccg gcatccacag gcagttcttc tccaactgca ccgtggacag ggtccacttg 360gaggaccccc cagacgaggt tctcatcccg ctgatcgtta tacccgtcgt tctgactgtc 420gccatggctg gcctggtggt gtggcgcagc aaacgcaccg acacgctgct gtgagggtcc 480cggtgagatg gagtgggtca cacctggcaa gctggaagaa agttccctgg ggatgggaga 540gcgggtgggt gctgccaatc tccagctact gtggccacac cccacctggt catgggcaga 600cccctccctt cctgggctga cctgctccct cgaggccagc ctgctccctg gctgaggctc 660aggctatccg cccaagctct ttgctcattc tagggccagt ggaggaaaat gtgataaggc 720cagagcttgt gtgctgggca cagaaatcac ctgctgcatc ctgtgctccg caggctgggc 780cggagcctct gcccgcaggt ttctatgctg tttcttagca cagaatccag cctagcctta 840gccgcagtct aggccctgct tggactagga ctccttgctt gaccccatct ctggttcctg 900ccctggctcc tgcaccagcc ccagctcctg cctacatcca ggcagaaaga taggcagggg 960ctcttggaag acgttccgtg ctgtgacctc cgagccctcc tggtgggaag acagctggaa 1020aggctgggag gagaagggag gggttggggg ttcccaggag ccatgcgtgg cctgcagagt 1080ccattccatc atgatgctgt gcccgctatg ggctgtgtcc atgaccagag gctggagtgg 1140gggtgtgtta gagcccctca ccgggacttg ctgtgcggat ggggcctggg cctccttcct 1200acaggggctc ctctgtgggt gaggggccct ctggaatggc atcccatgag cttgtggcct 1260ctatctgcta ccatctgtgt tttatctgag taaagttacc ttacttctgg aaaaaaaaaa 1320175778DNAHomo sapiensmisc_featureIncyte Clone No 2280639 175gcgctccctc gctggcggac ggctgggcgg cgggccgggc ccggggccgc ttggaatggc 60gcctcctccg ccttcgcccc aactgcttct cctggcagcc ctcgcgaggc tcctgggtcc 120cagcgaggtg atggctggac cggcggagga ggcgggagcc cattgtcccg agagcctgtg 180gcctctgcct ccgcaggtgt caccaagagt gacctacaca cgagtgagcc cagggcaggc 240tgaggatgtc accttcctct accacccctg tgcccatccc tggctgaagc tccagcttgc 300cctcctggcc tatgcttgta tggctaaccc ttccctcacc cctgacttca gcctcacgca 360ggatcggccc ctggtgctga ctgcatgggg gctggcgctg gagatggcct gggtagagcc 420agcctgggct gcccactggc tgatgaggag gcggaggagg aagcagagga agaagaaggc 480atggatctac tgtgaaagcc tttcagggcc tgctccctcc gagccaactc ccggtagagg 540gaggctgtgc cgaagagggt gtgtgcaggc cctggctctg gcctttgctc tgcggactgg 600cggccccctg gcacagaggt gacatctcaa gggcccaggc agccctcttc tagtggtgcc 660aagacgcgga tgctgcgggc tgcacttggg tcccagccca ctcgctcagc cctgaggttt 720ccctctgctt ccccagttag cttgatggcc aagcattcca tggcgggcta tcctggtt 7781761477DNAHomo sapiensmisc_featureIncyte Clone No 2292356 176cctggcctgg ctcgctgggg cctggggagc tgcccgtgct tccagcccag tctccccctg 60gctgctgccg gctgctggcc actcccacct cccaggcctg gcgtgaggcc cacagctgct 120gttgcacaac cctggtcatt gtgtgatggg gggaggcctg ggcctggccc gcccctctgc 180cagggcttca gacccctgcc cagccccagt atctgaagga accacagtgg agccaagccc 240gcgatgtgga gaactcaggc ttcaggagac cctggccctg ctcctggcgg ctccgggtgg 300ctttcagctc tctctgcaac ctgagctggg ggaggagcca ggcctcatgc ccagggctgg 360gagtggggag cctggtgtgc acgcgtgccc aggcctgcac gtggaccgac caggggaggg 420gcccagagct ctggctgggt cacccgcacc ccgcccccat ctcctccaga gccaccccag 480gaaaagcccg gctggacgag gtcatggctg ccgctgccct tacaagcctg tccaccagcc 540ctctccttct gggggcccca gttgcagcct tcagcccaga gcctggcctg gagccctgga 600aggaggccct ggtgcggccc ccaggcagct acagcagcag cagcaacagt ggagactggg 660gatgggacct ggccagtgac cagtcctctc cgtccacccc gtcaccccca ctgccccccg 720aggcagccca ctttctgttt ggggagccca ccctgagaaa aaggaagagc ccggcccagg 780tcatgttcca gtgtctgtgg aagagctgcg ggaaggtgct gagcacggcg tcggcgatgc 840agagacacat ccgcctggtg cacctggggt gcggcggggc ctggggtgcg gcggggcctg 900cgggttggct ggggttgtta ggcccggcca ggccacccct tcagctccca ctggctggct 960gtgtctcccg caggaggcag gcagagcctg agcagagtga tggtgaggag gacttctact 1020acacagagct ggatgttggt gtggacacgc tgaccgacgg gctgtccagc ctgactccag 1080ttttccccga gggcttccat gccagcttgc cttcccccgc cctgaagctc cgcagacttg 1140gtgggacccg ccaaccccgc cagtacccct gaggagcgcc gggatttagt cgaggtcctt 1200tgtcggcgcc cacggggaat attaatagct cccggggggg gggaatactt ttgaaggcag 1260ttgataaaaa attttccccc ccaaacagag ggaggcccga gaataaagaa cccctccggg 1320aaaaaacaca gtgggagaca tagagttgat tctccctggg tgagaaaaat ttgggtaaag 1380cggcttcaag caatttcgca gagcaagatt tgcgggcgcc ggaacccata aaggtggtaa 1440aaccctgggg ggtccccaag agggggaagc tcaaccc 1477177682DNAHomo sapiensmisc_featureIncyte Clone No 2349310 177tctgaatgtt ttggtgaata aatctgttct tcagcaaccc tacctgcttc tccaaactgc 60ctaaagagat ccagtactga tgacgctgtt cttccatctt tactccctgg aaactaacca 120cgttgtcttc tttccttcac caccacccag gagctcagag atctaagctg ctttccatct 180tttctcccag ccccaggaca ctgactctgt acaggatggg gccgtcctct tgcctccttc 240tcatcctaat cccccttctc cagctgatca acctggggag tactcagtgt tccttagact 300ccgttatgga taagaagatc aaggatgttc tcaacagtct agagtacagt ccctctccta 360taagcaagaa gctctcgtgt gctagtgtca aaagccaagg cagaccgtcc tcctgccctg 420ctgggatggc tgtcactggc tgtgcttgtg gctatggctg tggttcgtgg gatgttcagc 480tggaaaccac ctgccactgc cagtgcagtg tggtggactg gaccactgcc cgctgctgcc 540acctgacctg acagggagga ggctgagaac tcagttttgt gaccatgaca gtaatgaaac 600cagggtccca accaagaaat ctaactcaaa cgtcccactt catttgttcc attcctgatt 660cttgggtaat aaagacaaac tt 6821781508DNAHomo sapiensmodified_base(11)a, c, g, t, unknown, or othermodified_base(139)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 2373227 178gcgtaacccc ntgatctggt gataaacgta ttacccgctt ttgagtgagc tgataccgct 60cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg agaaagcgga agagcgccca 120atacgcaaac cgcttctcnc cgcgcgttgg ccgattcatt aatcagcttg cacgacaggt 180ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcccc 240acccccttcc ccgcgggcct cggttcaaac gacccggtgg gtctacagcg gaagggaggg 300agcgaaggta ggaggcaggg cttgcctcac tggccaccct cccaacccca agagcccagc 360cccatggtcc ccgccgccgg cgcgctgctg tgggtcctgc tgctgaatct gggtccccgg 420gcggcggggg cccaaggcct gacccagact ccgaccgaaa tgcagcgggt cagtttacgc 480tttgggggcc ccatgacccg cagctaccgg agcaccgccc ggactggtct tccccggaag 540acaaggataa tcctagagga cgagaatgat gccatggccg acgccgaccg cctggctgga 600ccagcggctg ccgagctctt ggccgccacg gtgtccaccg gctttagccg gtcgtccgcc 660attaacgagg aggatgggtc ttcagaagag ggggttgtga ttaatgccgg aaaggatagc 720accagcagag agcttcccag tgcgactccc aatacagcgg ggagttccag cacgaggttt 780atagccaata gtcaggagcc tgaaatcagg ctgacttcaa gcctgccgcg ctcccccggg 840aggtctactg aggacctgcc aggctcgcag gccaccctga gccagtggtc cacacctggg 900tctaccccga gccggtggcc gtcaccctca cccacagcca tgccatctcc tgaggatctg 960cggctggtgc tgatgccctg gggcccgtgg cactgccact gcaagtcggg caccatgagc 1020cggagccggt ctgggaagct gcacggcctt tccgggcgcc ttcgagttgg ggcgctgagc 1080cagctccgca cggagcacaa gccttgcacc tatcaacaat gtccctgcaa ccgacttcgg 1140gaagagtgcc ccctggacac aagtctctgt actgacacca actgtgcctc tcagagcacc 1200accagtacca ggaccaccac tacccccttc cccaccatcc acctcagaag cagtcccagc 1260ctgccacccg ccagcccctg cccagccctg gctttttgga

aacgggtcag gattggcctg 1320gaggatattt ggaatagcct ctcttcagtg ttcacagaga tgcaaccaat agacagaaac 1380cagaggtaat ggccacttca tccacatgag gagatgtcag tatctcaacc tctcttgccc 1440tttcaatcct agcacccact agatattttt agtacagaaa aacaaaactg gaaaacaaaa 1500aaaaaaaa 1508179558DNAHomo sapiensmisc_featureIncyte Clone No 2457682 179ggagaaagga tggccggcct ggcggcgcgg ttggtcctgc tagctggggc agcggcgctg 60gcgagcggct cccagggcga ccgtgagccg gtgtaccgcg actgcgtact gcagtgcgaa 120gagcagaact gctctggggg cgctctgaat cacttccgct cccgccagcc aatctacatg 180agtctagcag gctggacctg tcgggacgac tgtaagtatg agtgtatgtg ggtcaccgtt 240gggctctacc tccaggaagg tcacaaagtg cctcagttcc atggcaagtg gcccttctcc 300cggttcctgt tctttcaaga gccggcatcg gccgtggcct cgtttctcaa tggcctggcc 360agcctggtga tgctctgccg ctaccgcacc ttcgtgccag cctcctcccc catgtaccac 420acctgtgtgg ccttcgcctg gctttctgga agatgacagc ctgtagctgc tgaaggaatc 480agaggacaag ttcaggctgg actgaagacc cttggagcga gtcttcccca gttggggata 540ctgcccccgc cctgctgg 558180502DNAHomo sapiensmisc_featureIncyte Clone No 2480426 180cttggagtct gggaggagga aagcggagcc ggcagggagc gaaccaggac tggggtgacg 60gcagggcagg gggcgcctgg ccggggagaa gcgcgggggc tggagcacca ccaactggag 120ggtccggagt agcgagcgcc ccgaaggagg ccatcgggga gccgggaggg gggactgcga 180gaggaccccg gcgtccgggc tcccggtgcc agcgctatga ggccactcct cgtcctgctg 240ctcctgggcc tggcggccgg ctcgccccca ctggacgaca acaagatccc cagcctctgc 300ccgggactgc cgggacctcg aggggacccc gggccgcgag gagaggcggg acccgcgggg 360cccaccgggc tagccgggga gtgctcggtg cctccgcgat ccgccttcag cgccaagcgc 420tccgagatcc gggtgcctcc gctgtctgac gcacccttgc cttcgaccgc gtgctggtga 480acgagcaagg acattacgac gc 5021811659DNAHomo sapiensmisc_featureIncyte Clone No 2503743 181gctgtgcggc ggggcaggca tgggagccgc gcgctctctc ccggcgccca cacctgtctg 60agcggcgcac gagccgcggc ccgggcgggc tgctcggcgc ggaacagtgc tcggcatggc 120agggattcca gggctcctct tccttctctt ctttctgctc tgtgctgttg ggcaagtgag 180cccttacagt gccccctgga aacccacttg gcctgcatac cgcctccctg tcgtcttgcc 240ccagtctacc ctcaatttag ccaagccaga ctttggagcc gaagccaaat tagaagtatc 300ttcttcatgt ggaccccagt gtcataaggg aactccactg cccacttacg aagaggccaa 360gcaatatctg tcttatgaaa cgctctatgc caatggcagc cgcacagaga cgcaggtggg 420catctacatc ctcagcagta gtggagatgg ggcccaacac cgagactcag ggtcttcagg 480aaagtctcga aggaagcggc agatttatgg ctatgacagc aggttcagca tttttgggaa 540ggacttcctg ctcaactacc ctttctcaac atcagtgaag ttatccacgg gctgcaccgg 600caccctggtg gcagagaagc atgtcctcac agctgcccac tgcatacacg atggaaaaac 660ctatgtgaaa ggaacccaga agcttcgagt gggcttccta aagcccaagt ttaaagatgg 720tggtcgaggg gccaacgact ccacttcagc catgcccgag cagatgaaat ttcagtggat 780ccgggtgaaa cgcacccatg tgcccaaggg ttggatcaag ggcaatgcca atgacatcgg 840catggattat gattatgccc tcctggaact caaaaagccc cacaagagaa aatttatgaa 900gattggggtg agccctcctg ctaagcagct gccagggggc agaattcact tctctggtta 960tgacaatgac cgaccaggca atttggtgta tcgcttctgt gacgtcaaag acgagaccta 1020tgacttgctc taccagcaat gcgatgccca gccaggggcc agcgggtctg gggtctatgt 1080gaggatgtgg aagagacagc agcagaagtg ggagcgaaaa attattggca ttttttcagg 1140gcaccagtgg gtggacatga atggttcccc acaggatttc aacgtggctg tcagaatcac 1200tcctctcaaa tatgcccaga tttgctattg gattaaagga aactacctgg attgtaggga 1260ggggtgacac agtgttccct cctggcagca attaagggtc ttcatgttct tattttagga 1320gaggccaaat tgttttttgt cattggcgtg cacacgtgtg tgtgtgtgtg tgtgtgtgtg 1380taaggtgtct tataatcttt tacctatttc ttacaattgc aagatgactg gctttactat 1440ttgaaaactg gtttgtgtat catatcatat atcatttaag cagtttgaag gcatactttt 1500gcatagaaat aaaaaaaata ctgatttggg gcaatgagga atatttgaca attaagttaa 1560tcttcacgtt tttgcaaact ttgattttta tttcatctga acttgtttca aagatttata 1620ttaaatattt ggcatacaag agatatgaaa aaaaaaaaa 16591822015DNAHomo sapiensmisc_featureIncyte Clone No 2537684 182aaaaacaaag gcaatgcatt ggcaagcctc acagcacaga gtgaccgctg cctggcgttc 60cccagcactc ggtgtggaaa ggcccctacc tgctgtaaga ttatgggtcc atgaaagcag 120taagctggac acagaggtgt agtgtgcggg acagagggcc ttgcagatgc ctttctgttg 180gtgttttagt gttaaaatac ggagagtatg gaactcttca cctccatttt ctcagcggct 240gtgaagcagc ctcctagctt cggaagtacg gacactacgt cgcgttttca agcgtgtctg 300ttctgcaggt aacagcatca agctgcacgt ggaagcatct cgcggttttc tagaaacagg 360cattttctta tccctctccc gctccttttt ccacaaaggt gaatttcata aatgtaatac 420tagtaaagtg aatgaattac tgagtttata cagaaattta ggtaacttct cctttagtct 480caagagcgag tcttgctttt taatgggtgc cgtttatgtt gctgcccgcc ctgtgtgcct 540ggctcctctg ggtgccttgg tgtctgctgg tggctggcag tgggcgcagc ggaggagagt 600tgtgctgcag ctcatacggt gtgtctgtca tctcagtctg gagtaaatgc agtgtctgcc 660ggtgtctgat gggttctgtc cctcgtattt tctttgcctt ctatcccatt gcctggctac 720cgctgcctgg cagccaaggg tgttggtcgc gaagctggga gtggcctctg gtggagcctg 780catcttgtct cgtctgcctc tgctttacat ttggtgtact ttcgggcgtg gtggcagtaa 840aatgacaccg tgattgagct tgtcagcaga gctgaaagag aaagtagaag gatgtgcatt 900gtttcttgta agatatcttg catgtatctg tgtattcaaa ttcaaacaga gatggtttgt 960ccatttgtcc actgagaaat tataaactag ggacaagggg gaggaaaagt actgaaatac 1020agtttatgaa gcaagtgtgt ctcgggctgt gcttgtccca ggagccccag cagcatctga 1080actgaggctt cttcagtcct gcaggaacag gatcatctgt ctcagcggtg ggcagatgtt 1140ttcatagaca gccagggagt aaacactgtt ggctctgtgg gctgtatggt ctctgccata 1200aatagtacag agatgtggct gtgtctagta caacttttag acacagaaat ctgaatgaca 1260tatattgttc tgtgtcaaga aacttagatt ttttttttaa ctatttaaaa acgtgaaacc 1320tattcttagc tcacaggcca tggagaagct ggtggggacc agacccagct ccttagctgg 1380ctgggctggg gagggggcag tgacagtggc agctgctact cactgctcag tgtggaaaac 1440acaggacttg gcaatcacag cccgcagaac catcatgtgt ggcagaagcc tgagggatgc 1500ggtttcttgc ccacgtgctc tgttcatttt ctgttgtttt tctgcactta aagaattcac 1560atggaagcat gttttataaa atgaattacc agagaaacag agatgggccg agattctcag 1620aaatggtccc atgtgaccaa gttctgctgt ttgggtgaca gtgctttgaa gatctccttt 1680gaggatgtgc agtctttttt tttttttttt tttgagatgg agtttgttgc ccaggctgga 1740gtgagtggca cagtctcggc tcactgcaac ctccacctcc tgggttcaag cagttctcgt 1800gccgcagcct cccaagtagc tgggactaca ggcatgcgcc accacgccag gctaattttt 1860gtatttttag tagagatggg gtttcaccat gtctcaaact cctgacctca ggcgatccac 1920ccacctcagc gtcccaaagt gctggggata taggggtgac cacccgcacc tgcgccaaga 1980gtgggctttt aattagggac aaatccaatg gaagg 2015183740DNAHomo sapiensmisc_featureIncyte Clone No 2593853 183ctgctttcgt gaagacaaga tgaagttcac aattgtcttt gctggacttc ttggagtctt 60tctagctcct gccctagcta actataatat caacgtcaat gatgacaaca acaatgctgg 120aagtgggcag cagtcagtga gtgtcaacaa tgaacacaat gtggccaatg ttgacaataa 180caacggatgg gactcctgga attccatctg ggattatgga aatggctttg ctgcaaccag 240actctttcaa aagaagacat gcattgtgca caaaatgaac aaggaagtca tgccctccat 300tcaatccctt gatgcactgg tcaaggaaaa gaagcttcag ggtaagggac caggaggacc 360acctcccaag ggcctgatgt actcagtcaa cccaaacaaa gtcgatgacc tgagcaagtt 420cggaaaaaac attgcaaaca tgtgtcgtgg gattccaaca tacatggctg aggagatgca 480agaggcaagc ctgttttttt actcaggaac gtgctacacg accagtgtac tatggattgt 540ggacatttcc ttctgtggag acacggtgga gaactaaaca attttttaaa gccactatgg 600atttagtcat ctgaatatgc tgtgcagaaa aaatatgggc tccagtggtt tttaccatgt 660cattctgaaa tttttctcta ctagttatgt ttgatttctt taagtttcaa taaaatcatt 720tagcattgaa aaaaaaaaaa 740184748DNAHomo sapiensmisc_featureIncyte Clone No 2622354 184ctgcaaccac ccagagccat ggctccccga ggctgcatcg tagctgtctt tgccattttc 60tgcatctcca ggctcctctg ctcacacgga gccccagtgg cccccatgac tccttacctg 120atgctgtgcc agccacacaa gagatgtggg gacaagttct acgaccccct gcagcactgt 180tgctatgatg atgccgtcgt gcccttggcc aggacccaga cgtgtggaaa ctgcaccttc 240agagtctgct ttgagcagtg ctgcccctgg accttcatgg tgaagctgat aaaccagaac 300tgcgactcag cccggacctc ggatgacagg ctttgtcgca gtgtcagcta atggaacatc 360aggggaacga tgactcctgg attctccttc ctgggtgggc ctggagaaag aggctggtgt 420tacctgagat ctgggatgct gagtggctgt ttgggggcca gagaaacaca cactcaactg 480cccacttcat tctgtgacct gtctgaggcc caccctgcag ctgccctgag gaggcccaca 540ggtccccttc tagaattctg gacagcatga gatgcgtgtg ctgatggggg cccagggact 600ctgaaccctc ctgatgaccc ctatggccaa catcaacccg gcaccacccc aaggctggct 660gggaaccctt cacccttctg tgagattttc catcatctca agttctcttc tatccaggag 720caaagcacag gatcataata aatttatg 748185648DNAHomo sapiensmisc_featureIncyte Clone No 2641377 185cggctcgagg atccccaagt ctctgaccca ccttcctgcc tgctcctctc ctcccacatt 60ggctcagatt ctttccccgc tgtctgtggg tccacactcc cagtggcacc tccaggagag 120aatctgattg gctcagttcg ccagataact caactttccc attggctacc tttgggtcag 180gtgatctcca ctagacctat cgcctatgcc tgatggtggg tcacatggtg caaatgttgc 240ctgagagctt agtggattag ggatgtggct gggctcatgg ttgacgtccc tgctgctgag 300cccttacggg tcaggctggg agaaggtacc atgttgtgtg actggtcatt tgaggtcttg 360cagctgttgc ttgctgggct tggcaggtgt tcaaagtgac catttttctg aagggttttt 420ttctgagtat tcctcagatg tactcccctg gggccgacgg tctttccttc cacagggcga 480tgcttcccta cttgcttgtg aatgtttcct tcatctccag gttgtctggg gacaattctg 540tcttttggag gcctgggcag gatttacaga gggctccatg ccagctcctt cctgccgggt 600ccacttctgg tgtagggtaa acacctgcgc attcatgtcc tagtgttg 6481862110DNAHomo sapiensmodified_base(1932)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 2674857 186cggcggccat ctttactcag ggcacagagg gtctctgcgg ccgtagcggc cggggctgcg 60gtagccactt tagatttggg caaggacttt agattcgggc tctgttctgt ttccgccgtc 120ctgcttcctg ccgaggctgg cccaggcagc cgcgcttcga aggacgccgc cgggagctgc 180ggacatgcgt ggagtggcag tgctaacggc tggtgtctcg cactgttggc ctgtgaaggt 240acgtgaagct gaaagcctgg aatggctgga aaggggtcat caggcaggcg gcccctgctg 300ctggggctgc tggtggccgt agccactgtc cacctggtca tctgtcccta caccaaagtg 360gaggagagct tcaacctgca ggccacacat gacctgctct accactggca agacctggag 420cagtacgacc atcttgagtt ccccggagtc gtccccagga cgttcctcgg gccagtggtg 480atcgcagtgt tctccagccc cgcggtttac gtgctttcgc tgttagaaat gtccaagttt 540tactctcagc taatagttag aggagtgctt ggactcggcg tgatttttgg actctggacg 600ttacaaaagg aagtgagacg gcacttcggg gccatggtgg ccaccatgtt ctgctgggtg 660acggccatgc agttccacct gatgttctac tgcacgcgga cactgcccaa tgtgctggcc 720ctgcctgtag tcctgctggc cctcgcggcc tggctgcggc acgagtgggc ccgcttcatc 780tggctgtcag ccttcgccat catcgtgttc agggtggagc tgtgcctgtt cctgggcctc 840ctgctgctgc tggccttggg caaccgaaag gtttctgtag tcagagccct tcgccacgcc 900gtcccggcag ggatcctctg tttaggactg acggttgctg tggactctta tttttggcgg 960cagctcactt ggccggaagg aaaggtgctt tggtacaaca ctgtcctgaa caaaagctcc 1020aactggggga cctccccgct gctgtggtac ttctactcag ccctgccccg cggcctgggc 1080tgcagcctgc tcttcatccc cctgggcttg gtagacagaa ggacgcacgc gccgacggtg 1140ctggcactgg gcttcatggc actctactcc ctcctgccac acaaggagct acgcttcatc 1200atctatgcct tccccatgct caacatcacg gctgccagag gctgctccta cctgctgaat 1260aactataaaa agtcttggct gtacaaagcg gggtctctgc ttgtgatcgg acacctcgtg 1320gtgaatgccg cctactcagc cacggccctg tatgtgtccc atttcaacta cccaggtggc 1380gtcgcaatgc agaggctgca ccagctggtg cccccccaga cagacgtcct tctgcacatt 1440gacgtggcag ccgcccagac aggtgtgtct cggtttctcc aagtcaacag cgcctggagg 1500tacgacaaga gggaggatgt gcagccgggg acaggcatgc tggcatacac acacatcctc 1560atggaggcgg cccctgggct cctggccctc tacagggaca cacaccgggt cctggccagc 1620gtcgtgggga ccacaggtgt gagtctgaac ctgacccaac tgcccccctt caacgtccac 1680ctgcagacaa agctggtgct tctggagagg ctcccccggc cgtcctgagg gggaccaggc 1740agccctcagc agccacaggc cttccaggag ctgttatcac taccagtttc tggcacaatt 1800ccagcacaat tatgacaatt cagagaagca agtcaaagga ctggggcacc tgcctctgac 1860agacaccaga ccaggtccag ggcctcctcc cacagcctca gctgggggct cttcagcaac 1920caaagaacga anggggcccc aagttctttg tttgggcacc ccccggggta agcccacttg 1980cccccaaggg tttgatgggg ttgggcccag cttccagggg ctttcccttg gccggggttt 2040gacttgttcc ggccccagga ttcaagggtt ggcccaattt cccattgaac ttaaatttcc 2100agggaaaggc 2110187773DNAHomo sapiensmisc_featureIncyte Clone No 2758485 187cccggagccg gggagggagg gagcgaggtt cggacaccgg cggcggctgc ctggcctttc 60catgagcccg cggcggaccc tcccgcgccc cctctcgctc tgcctctccc tctgcctctg 120cctctgcctg gccgcggctc tgggaagtgc gcagtccggg tcgtgtaggg ataaaaagaa 180ctgtaaggtg gtcttttccc agcaggaact gaggaagcgg ctaacacccc tgcagtacca 240tgtcactcag gagaaaggga ccgaaagtgc ctttgaagga gaatacacac atcacaaaga 300tcctggaata tataaatgtg ttgtttgtgg aactccattg tttaagtcag aaaccaaatt 360tgactccggt tcaggttggc cttcattcca cgatgtgatc aattctgagg caatcacatt 420cacagatgac ttttcctatg ggatgcacag ggtggaaaca agctgctctc agtgtggtgc 480tcaccttggg cacatttttg atgatgggcc tcgtccaact gggaaaagat actgcataaa 540ttcggctgcc ttgtctttta cacctgcgga tagcagtggc accgccgagg gaggcagtgg 600ggtcgccagc ccggcccagg cagacaaagc ggactctgag agtaatggag agtgatggaa 660acaaagtgta cttaatgcac agcttattaa aaagatcaaa attgttatcc taatagatat 720attttttcaa aaactataag ggcagttttg tgctattgta atttttcctc ctt 773188714DNAHomo sapiensmisc_featureIncyte Clone No 2763296 188gggagcctcc cacgctctcc agctcactcg gcaggcagcg gggaccaggg ctggcaggtt 60aagcctctgg gggtggatcc tgaaaggtgg tccagccgcc tggccctgcg tgggaccctc 120cacctggcag caggtggtga cttccaagag tgactccgtc ggaggaaaat gactccccag 180tcgctgctgc agacgacact gttcctgctg agtctgctct tcctggtcca aggtgcccac 240ggcaggggcc acagggaaga ctttcgcttc tgcagccagc ggaaccagac acacaggagc 300agcctccact actactggtc catgcggctg caggcccggg gtggcccctc ccctctgaag 360agcaactcag acagcgccag gctccccatc agctcgggca gcacctcgtc cagccgcatc 420taggcctcca gcccacctgc ccatgtaatg aagcagagat gcggcctcgt cgcacactgc 480ctgtagcccc cgaacccggc ccagccccag gccagtaagc cgcagacttt agaaagccca 540acgaccatgg agagatgggc cgttgccatg gtggacggac tcccgggctg ggcttttgag 600attggcttag gggctactcg gctctcactc agctcccacg ggactcaaga atgcggcgcc 660atgctgcctt aggtactgtc cccacatctg tcccaaccca gctggaggcc tggt 714189609DNAHomo sapiensmisc_featureIncyte Clone No 2779436 189cggccagggc gccgacagcc cgacctcacc aggagaacat gcagctcggc actgggctcc 60tgctggccgc cgtcctgagc ctgcagctgg ctgcagccga agccatatgg tgtcaccagt 120gcacgggctt cggagggtgc tcccatggat ccagatgcct gagggactcc acccactgtg 180tcaccactgc cacccgggtc ctcagcaaca ccgaggattt gcctctggtc accaagatgt 240gccacatagg ctgccccgat atccccagcc tgggcctggg cccctacgta tccatcgctt 300gctgccagac cagcctctgc aaccatgact gacggctgcc ctcctccagg cccccggacg 360ctcagccccc acagccccca cagcctggcg ccagggctca cggccgcccc tccctcgaga 420ctggccagcc cacctctccc ggcctctgca gccaccgtcc agcaccgctt gtcctaggga 480agtcctgcgt ggagtcttgc ctcaatctgc tgccgtccaa gcctggggcc catcgtgcct 540gccgcccctt caggtcccga cctccccaca ataaaatgtg attggatcgt gtggtacaaa 600aaaaaaaac 6091901088DNAHomo sapiensmisc_featureIncyte Clone No 2808528 190tgtagaagac agcggcgttg ccatggcggc gtctctgggg caggtgttgg ctctggtgct 60ggtggccgct ctgtggggtg gcacgcagcc gctgctgaag cgggcctccg ccggcctgca 120gcgggttcat gagccgacct gggcccagca gttgctacag gagatgaaga ccctcttctt 180gaatactgag tacctgatgc cctttctcct caaccagtgt ggatcccttc tctattacct 240caccttggca tcgacagatc tgaccctggc tgtgcccatc tgtaactctc tggctatcat 300cttcacactg attgttggga aggcccttgg agaagatatt ggtggaaaac gagcagttgc 360tggcatggtg ctcaccgtga taggaatttc actctgcatc acaagctcag tgagtaagac 420ccaggggcaa cagtctaccc tttgagtggg ccgaacccac ttccagctct gctgcctcca 480ggaagcccct gggccatgaa gtgctggcag tgagcggatg gacctagcac ttcccctctc 540tggccttagc ttcctcctct cttatgggga taacagctac ctcatggatc acaataagag 600aacaagagtg aaagagtttt gtaaccttca agtgctgttc agctgcgggg atttagcaca 660ggagactcta cgctcaccct cagcaacctt tctgccccag cagctctctt cctgctaaca 720tctcaggctc ccagcccagc caccattact gtggcctgat ctggactatc atggtggcag 780gttccatgga ctgcagaact ccagctgcat ggaaagggcc agctgcagac tttgagccag 840aaatgcaaac gggaggcctc tgggactcag tcagagcgct ttggctgaat gaggggtgga 900accgagggaa gaaggtgcgt cggagtggca gatgcaggaa atgagctgtc tattagcctt 960gcctgcccca cccatgaggt aggcagaaat cctcactgcc agcccctctt aaacaggtag 1020agagctgtga gccccagccc cacctgactc cagcacacct ggcgagtagt agctgtcaat 1080aaagctat 10881911377DNAHomo sapiensmisc_featureIncyte Clone No 2809230 191gcgggacttc ctgtgtcgta tttccaagga ctccaaagcg aggccgggga ctgaaggtgt 60gggtgtcgag ccctctggca gagggttaac ctgggtcaaa tgcacggatt ctcacctcgt 120acagttacgc tctcccgcgg cacgtccgcg aggacttgaa gtcctgagcg ctcaagtttg 180tccgtaggtc gagagaaggc catggaggtg ccgccaccgg caccgcggag ctttctctgt 240agagcattgt gcctatttcc ccgagtcttt gctgccgaag ctgtgactgc cgattcggaa 300gtccttgagg agcgtcagaa gcggcttccc tacgtcccag agccctatta cccggaatct 360ggatgggacc gcctccggga gctgtttggc aaagatgaac agcagagaat ttcaaaggac 420cttgctaata tctgtaagac ggcagctaca gcaggcatca ttggctgggt gtatggggga 480ataccagctt ttattcatgc taaacaacaa tacattgagc agagccaggc agaaatttat 540cataaccggt ttgatgctgt gcaatctgca catcgtgctg ccacacgagg cttcattcgt 600tatggctggc gctggggttg gagaactgca gtgtttgtca ctatattcaa cacagtgaac 660actagtctga atgtataccg aaataaagat gccttaagcc attttgtaat tgcaggagct 720gtcacgggaa gtctttttag gataaacgta ggcctgcgtg gcctggtggc tggtggcata 780attggagcct tgctgggcac tcctgtagga ggcctgctga tggcatttca gaagtactct 840ggtgagactg ttcaggaaag aaaacagaag gatcgaaagg cactccatga gctaaaactg 900gaagagtgga aaggcagact acaagttact gagcacctcc ctgagaaaat tgaaagtagt 960ttacaggaag atgaacctga gaatgatgct aagaaaattg aagcactgct aaaccttcct 1020agaaaccctt cagtaataga taaacaagac aaggactgaa agtgctctga acttgaaact 1080cactggagag ctgaagggag ctgccatgtc cgatgaatgc caacagacag gccactcttt 1140ggtcagcctg ctgacaaatt taagtgctgg tacctgtggt ggcagtggct tgctcttgtc 1200tttttctttt ctttttaact aagaatgggg ctgttgtact ctcactttac ttatccttaa 1260atttaaatac atacttatgt ttgtattaat ctatcaatat atgcatacat gaatatatcc 1320acccacctag attttaagca gtaaataaaa catttcgcaa aagattaaaa aaaaaaa 1377192985DNAHomo sapiensmisc_featureIncyte Clone No 2816821 192gcgggcccgc gagtccgaga

cctgtcccag gagctccagc tcacgtgacc tgtcactgcc 60tcccgccgcc tcctgcccgc gccatgaccc agccggtgcc ccggctctcc gtgcccgccg 120cgctggccct gggctcagcc gcactgggcg ccgccttcgc cactggcctc ttcctgggga 180ggcggtgccc cccatggcga ggccggcgag agcagtgcct gcttcccccc gaggacagcc 240gcctgtggca gtatcttctg agccgctcca tgcgggagca cccggcgctg cgaagcctga 300ggctgctgac cctggagcag ccgcaggggg attctatgat gacctgcgag caggcccagc 360tcttggccaa cctggcgcgg ctcatccagg ccaagaaggc gctggacctg ggcaccttca 420cgggctactc cgccctggcc ctggccctgg cgctgcccgc ggacgggcgc gtggtgacct 480gcgaggtgga cgcgcagccc ccggagctgg gacggcccct gtggaggcag gccgaggcgg 540agcacaagat cgacctccgg ctgaagcccg ccttggagac cctggacgag ctgctggcgg 600cgggcgaggc cggcaccttc gacgtggccg tggtggatgc ggacaaggag aactgctccg 660cctactacga gcgctgcctg cagctgctgc gacccggagg catcctcgcc gtcctcagag 720tcctgtggcg cgggaaggtg ctgcaacctc cgaaagggga cgtggcggcc gagtgtgtgc 780gaaacctaaa cgaacgcatc cggcgggacg tcagggtcta catcagcctc ctgcccctgg 840gcgatggact caccttggcc ttcaagatct agggctggcc cctagtgagt gggctcgagg 900gagggttgcc tgggaacccc aggaattgac cctgagtttt aaattcgaaa ataaagtggg 960gctgggacac acgaaaaaaa aaaaa 9851931310DNAHomo sapiensmisc_featureIncyte Clone No 2817268 193cccacgcgtc cgggttcacg taaagacagc gagatcctga gggccagccg ggaaggaggc 60gtggatatgg agctggctgc tgccaagtcc ggggcccgcg ccgctgccta gcgcgtcctg 120gggactctgt ggggacgcgc cccgcgccgc ggctcgggga cccgtagagc ccggcgctgc 180gcgcatggcc ctgctctcgc gccccgcgct caccctcctg ctcctcctca tggccgctgt 240tgtcaggtgc caggagcagg cccagaccac cgactggaga gccaccctga agaccatccg 300gaacggcgtt cataagatag acacgtacct gaacgccgcc ttggacctcc tgggaggcga 360ggacggtctc tgccagtata aatgcagtga cggatctaag cctttcccac gttatggtta 420taaaccctcc ccaccgaatg gatgtggctc tccactgttt ggtgttcatc ttaacattgg 480tatcccttcc ctgacaaagt gttgcaacca acacgacagg tgctatgaaa cctgtggcaa 540aagcaagaat gactgtgatg aagaattcca gtattgcctc tccaagatct gccgagatgt 600acagaaaaca ctaggactaa ctcagcatgt tcaggcatgt gaaacaacag tggagctctt 660gtttgacagt gttatacatt taggttgtaa accatatctg gacagccaac gagccgcatg 720caggtgtcat tatgaagaaa aaactgatct ttaaaggaga tgccgacagc tagtgacaga 780tgaagatgga agaacataac ctttgacaaa taactaatgt ttttacaaca taaaactgtc 840ttatttttgt gaaaggatta ttttgagacc ttaaaataat ttatatcttg atgttaaaac 900ctcaaagcaa aaaaagtgag ggagatagtg aggggagggc acgcttgtct tctcaggtat 960cttccccagc attgctccct tacttagtat gccaaatgtc ttgaccaata tcaaaaacaa 1020gtgcttgttt agcggagaat tttgaaaaga ggaatatata actcaatttt cacaaccaca 1080tttaccaaaa aaagagatca aatataaaat tcatcataat gtctgttcaa cattatctta 1140tttggaaaat ggggaaatta tcacttacaa gtatttgttt actatgaaat tttaaataca 1200catttatgcc tagaaggaac ggactttttt tttctatttt aattacacat aatatgtaat 1260taaagtacaa cataatatgt tgtttctctg tagcccgttg agcatatgag 1310194914DNAHomo sapiensmisc_featureIncyte Clone No 2923165 194cggtggccat gactgcggcc gtgttcttcg gctgcgcctt cattgccttc gggcctgcgc 60tcgcccttta tgtcttcacc atcgccaccg agccgttgcg tatcatcttc ctcatcgccg 120gagctttctt ctggttggtg tctctactga tttcgtccct tgtttggttc atggcaagag 180tcattattga caacaaagat ggaccaacac agaaatatct gctgatcttt ggagcgtttg 240tctctgtcta tatccaagaa atgttccgat ttgcatatta taaactctta aaaaaagcca 300gtgaaggttt gaagagtata aacccaggtg agacagcacc ctctatgcga ctgctggcct 360atgtttctgg cttgggcttt ggaatcatga gtggagtatt ttcctttgtg aataccctat 420ctgactcctt ggggccaggc acagtgggca ttcatggaga ttctcctcaa ttcttccttt 480attcagcttt catgacgctg gtcattatct tgctgcatgt attctggggc attgtatttt 540ttgatggctg tgagaagaaa aagtggggca tcctccttat cgttctcctg acccacctgc 600tggtgtcagc ccagaccttc ataagttctt attatggaat aaacctggcg tcagcattta 660taatcctggt gctcatgggc acctgggcat tcttagctgc gggaggcagc tgccgaagcc 720tgaaactctg cctgctctgc caagacaaga actttcttct ttacaaccag cgctccagat 780aacctcaggg aaccagcact tcccaaaccg cagactacat ctttagagga agcacaactg 840tgcctttttc tgaaaatccc tttttctggt ggaattgaga aagaaataaa actatgcaga 900tatgaaaaaa aaaa 914195606DNAHomo sapiensmisc_featureIncyte Clone No 2949822 195ttttttaata atgcctttta gttggatggt aattatcctg ggttttctat gtggattatc 60aggtcagctt caaataatga acaccctctc ttctcttcca attgttttac ttgtttcttc 120ttcttgtctt atattagcca gaatgtcata tagtatattg accagtagct atggtggtgg 180cgtttttatc ttattggact taaaaagaaa tacatcaaaa gtttctccat taatgatgat 240gtttgctata gggcattgat agatagcctt caaaaagtta agaaagttct tttctttcta 300gtcttcaagg ttaaaaagtt tttaaagatc ttaattgaat gtgaacttta tcaaatgcct 360ttgtgatgtc tatggagata atcatgtatt tgcttcttta atacattcct gtggtgaaat 420atgtgaataa gtgttctgat attgaattat ctttgcattt ctagaataag ccctaataag 480tactattcaa ggtatttttc tcaaacacct gattggactc tgtaagctca tatttcattg 540agtggatttc cttctatgtt tgtcagtgca attgcgctat aattcgcgtt gctgtcctca 600tctgaa 606196893DNAHomo sapiensmisc_featureIncyte Clone No 2992192 196ccaccccgga agtcggctgg ccatggcggc gccttggagg cgatggccca cggggctgct 60agccgtgctg cggcccctgc tcacctgccg gcccctgcaa ggcacgacgc tgcaacggga 120tgtgctgctc tttgagcatg atcggggccg cttcttcacc atcctcgggc tgttctgcgc 180gggccagggc gtcttctggg cttccatggc tgtggcagcc gtgtcccggc ccccggttcc 240ggtgcagcct ctggatgcgg aggtcccaaa tcgtggcccc ttcgacctgc gctccgcgct 300ctggcgctac ggtctggccg tcggctgcgg cgccatcgga gccctcgtac tcggtgctgg 360tcttctcttc tctctccggt ctgtgcgctc agtggtgctt cgagctggag ggcagcaggt 420gaccctcacc actcatgccc cctttggctt gggggcccat ttcacagttc ctttgaagca 480ggtatcttgc atggcccacc ggggtgaagt ccctgccatg ctacctctga aagtcaaagg 540ccgacgcttc tatttcctct tggacaaaac tggacacttc cctaacacaa aactctttga 600caatactgtg ggtgcctacc ggagcttgtg aagaaatgac ctcaagtcac tcacctctcc 660aagaggagga taaaaactga accttgggga gccaggtgtg ttggttcaca cctgttgtaa 720tcccagcact ttgggagggt gaggcaggag cactgctcga gcccaggctg ggcaacatag 780cgagaccttg tctctattta caaaaaaaaa aacaaaaaaa aacgccaatc ttagaatgga 840gtaacaacca gggtcacaca aggaggtcaa gattcattaa caacaaataa agg 8931971730DNAHomo sapiensmisc_featureIncyte Clone No 2992458 197ggccagaggc tgcccggctc ccggaagcag gctgtgaggg gcgggagcgc tgctggaacc 60cgagccggag ccggagccac agcggggagg gtggcctggc ggcctggagc cggacgtgtc 120cggggcgtcc ccgcagaccg gggcagcagg tcgtccgggg gcccaccatg ctggtgactg 180cctaccttgc ttttgtaggc ctcctggcct cctgcctggg gctggaactg tcaagatgcc 240gggctaaacc ccctggaagg gcctgcagca atccctcctt ccttcggttt caactggact 300tctatcaggt ctacttcctg gccctggcag ctgattggct tcaggccccc tacctctata 360aactctacca gcattactac ttcctggaag gtcaaattgc catcctctat gtctgtggcc 420ttgcctctac agtcctcttt ggcctagtgg cctcctccct tgtggattgg ctgggtcgca 480agaattcttg tgtcctcttc tccctgactt actcactatg ctgcttaacc aaactctctc 540aagactactt tgtgctgcta gtggggcgag cacttggtgg gctgtccaca gccctgctct 600tctcagcctt cgaggcctgg tatatccatg agcacgtgga acggcatgac ttccctgctg 660agtggatccc agctaccttt gctcgagctg ccttctggaa ccatgtgctg gctgtagtgg 720caggtgtggc agctgaggct gtagccagct ggatagggct ggggcctgta gcgccctttg 780tggctgccat ccctctcctg gctctggcag gggccttggc ccttcgaaac tggggggaga 840actatgaccg gcagcgtgcc ttctcaagga cctgtgctgg aggcctgcgc tgcctcctgt 900cggaccgccg cgtgctgctg ttgggcacca tacaagctct atttgagagt gtcatcttca 960tctttgtctt cctctggaca cctgtgctgg acccacacgg ggcccctctg ggcattatct 1020tctccagctt catggcagcc agcctgcttg gctcttccct gtaccgtatc gccacctcca 1080agaggtacca ccttcagccc atgcacctgc tgtcccttgc tgtgctcatc gtcgtcttct 1140ctctcttcat gttgactttc tctaccagcc caggccagga gagtccggtg gagtccttca 1200tagcctttct acttattgag ttggcttgtg gattatactt tcccagcatg agcttcctac 1260ggagaaaggt gatccctgag acagagcagg ctggtgtact caactggttc cgggtacctc 1320tgcactcact ggcttgccta gggctccttg tcctccatga cagtgatcga aaaacaggca 1380ctcggaatat gttcagcatt tgctctgctg tcatggtgat ggctctgctg gcagtggtgg 1440gactcttcac cgtggtaagg catgatgctg agctgcgggt accttcacct actgaggagc 1500cctatgcccc tgagctgtaa ccccactcca ggacaagata gctgggacag actcttgaat 1560tccagctatc cgggattgta cagatctctc tgtgactgac tttgtgactg tcctgtggtt 1620tctcctgcca ttgctttgtg tttgggagga catgatgggg gtgatggact ggaaagaagg 1680tgccaaaagt tccctctgtg ttactcccat ttagaaaata aacactttta 17301982029DNAHomo sapiensmisc_featureIncyte Clone No 3044710 198ccttgacaag tcagaagctt gaaagcaggg aaatccggat gtctcggtta tgaagtggag 60cagtgagtgt gagcctcaac atagttccag aactctccat ccggactagt tattgagcat 120ctgcctctca tatcaccagt ggccatctga ggtgtttccc tggctctgaa ggggtaggca 180cgatggccag gtgcttcagc ctggtgttgc ttctcacttc catctggacc acgaggctcc 240tggtccaagg ctctttgcgt gcagaagagc tttccatcca ggtgtcatgc agaattatgg 300ggatcaccct tgtgagcaaa aaggcgaacc agcagctgaa tttcacagaa gctaaggagg 360cctgtaggct gctgggacta agtttggccg gcaaggacca agttgaaaca gccttgaaag 420ctagctttga aacttgcagc tatggctggg ttggagatgg attcgtggtc atctctagga 480ttagcccaaa ccccaagtgt gggaaaaatg gggtgggtgt cctgatttgg aaggttccag 540tgagccgaca gtttgcagcc tattgttaca actcatctga tacttggact aactcgtgca 600ttccagaaat tatcaccacc aaagatccca tattcaacac tcaaactgca acacaaacaa 660cagaatttat tgtcagtgac agtacctact cggtggcatc cccttactct acaatacctg 720cccctactac tactcctcct gctccagctt ccacttctat tccacggaga aaaaaattga 780tttgtgtcac agaagttttt atggaaacta gcaccatgtc tacagaaact gaaccatttg 840ttgaaaataa agcagcattc aagaatgaag ctgctgggtt tggaggtgtc cccacggctc 900tgctagtgct tgctctcctc ttctttggtg ctgcagctgg tcttggattt tgctatgtca 960aaaggtatgt gaaggccttc ccttttacaa acaagaatca gcagaaggaa atgatcgaaa 1020ccaaagtagt aaaggaggag aaggccaatg atagcaaccc taatgaggaa tcaaagaaaa 1080ctgataaaaa cccagaagag tccaagagtc caagcaaaac taccgtgcga tgcctggaag 1140ctgaagttta gatgagacag aaatgaggag acacacctga ggctggtttc tttcatgctc 1200cttaccctgc cccagctggg gaaatcaaaa gggccaaaga accaaagaag aaagtccacc 1260cttggttcct aactggaatc agctcaggac tgccattgga ctatggagtg caccaaagag 1320aatgcccttc tccttattgt aaccctgtct ggatcctatc ctcctacctc caaagcttcc 1380cacggccttt ctagcctggc tatgtcctaa taatatccca ctgggagaaa ggagttttgc 1440aaagtgcaag gacctaaaac atctcatcag tatccagtgg taaaaaggcc tcctggctgt 1500ctgaggctag gtgggttgaa agccaaggag tcactgagac caaggctttc tctactgatt 1560ccgcagctca gaccctttct tcagctctga aagagaaaca cgtatcccac ctgacatgtc 1620cttctgagcc cggtaagagc aaaagaatgg cagaaaagtt tagcccctga aagccatgga 1680gattctcata acttgagacc taatctctgt aaagctaaaa taaagaaata gaacaaggct 1740gaggatacga cagtacactg tcagcaggga ctgtaaacac agacagggtc aaagtgtttt 1800ctctgaacac attgagttgg aatcactgtt tagaacacac acacttactt tttctggtct 1860ctaccactgc tgatattttc tctaggaaat atacttttac aagtaacaaa aataaaaact 1920cttataaatt tctattttta tctgagttac agaaatgatt actaaggaag attactcagt 1980aatttgttta aaaagtaata aaattcaaca aacatttaaa aaaaaaaaa 2029199543DNAHomo sapiensmisc_featureIncyte Clone No 3120415 199ccggcgctgg aggggcgagg accgggtata agaagcctcg tggccttgcc cgggcagccg 60caggttcccc gcgcgccccg agcccccgcg ccatgaagct cgccgccctc ctggggctct 120gcgtggccct gtcctgcagc tccgctgctg ctttcttagt gggctcggcc aagcctgtgg 180cccagcctgt cgctgcgctg gagtcggcgg cggaggccgg ggccgggacc ctggccaacc 240ccctcggcac cctcaacccg ctgaagctcc tgctgagcag cctgggcatc cccgtgaacc 300acctcataga gggctcccag aagtgtgtgg ctgagctggg tccccaggcc gtgggggccg 360tgaaggccct gaaggccctg ctgggggccc tgacagtgtt tggctgagcc gagactggag 420catctacacc tgaggacaag acgctgccca cccgcgaggg ctgaaaaccc cgccgcgggg 480aggaccgtcc atccccttcc cccggcccct ctcaataaac gtggttaaga gcaaaaaaaa 540aaa 543200531DNAHomo sapiensmisc_featureIncyte Clone No 126758 200gcaagtggaa ccactggctt ggtggatttt gctagatttt tctgattttt aaactcctga 60aaaatatccc agataactgt catgaagctg gtaactatct tcctgctggt gaccatcagc 120ctttgtagtt actctgctac tgccttcctc atcaacaaag tgccccttcc tgttgacaag 180ttggcacctt tacctctgga caacattctt ccctttatgg atccattaaa gcttcttctg 240aaaactctgg gcatttctgt tgagcacctt gtggaggggc taaggaagtg tgtaaatgag 300ctgggaccag aggcttctga agctgtgaag aaactgctgg aggcgctatc acacttggtg 360tgacatcaag ataaagagcg gaggtggatg gggatggaag atgatgctcc tatcctccct 420gcctgaaacc tgttctacca attatagatc aaatgcccta aaatgtagtg acccgtgaaa 480aggacaaata aagcaatgaa tacatttaaa ctcagaccat cgaatggaaa a 531201491DNAHomo sapiensmisc_featureIncyte Clone No 674760 201cttctcccat gactgccggc cagtttcctg cacttgtttc tctcgcttta ttgctggacg 60gtgggagaag ggcaagtgca agacggaatc gagggcacct ctgggtgttc tgtacctctt 120ttcttcttgc accttgggaa gtggaggacg tgggatggaa gaagggcctg gacctccctc 180cttcctcctc cccaccttct cctaaggagc ttgccctgca gtaagcccca actttccctt 240cctcttttcc ctctatcaga gtcgtcgccc accccccttt cccaccgctc ccctaccccc 300gccttcctgc caagccgagg gcgacggtga tccccagctt agtaagaaaa gtaaataggc 360cgggcgcggt agctcacgcc tggaatccca gcactgtggg aggccgaggc gggcggatcg 420cttgagccca ggagatcagg ttggagacag cctaggcaac atggcgaaac cctgtctcta 480caaaaaaaaa a 4912021551DNAHomo sapiensmisc_featureIncyte Clone No 1229438 202ccggggggcc ggcggcggcc cgggcgggac gatgaagcgg cagaacgtgc gcacgctggc 60gctcatcgtg tgcaccttca cctacctgct ggtgggcgcc gcggtcttcg acgcgctgga 120gtcggagccc gagctgatcg agcggcagcg gctggagctg cggcagcagg agctgcgggc 180gcgctacaac ctcagccagg gcggctacga ggagctggag cgcgtcgtgc tgcgcctcaa 240gccgcacaag gccggcgtgc agtggcgctt cgccggctcc ttctacttcg ccatcaccgt 300catcaccacc atcggctacg ggcacgcggc acccagcacg gatggcggca aggtgttctg 360catgttctac gcgctgctgg gcatcccgct cacgctcgtc atgttccaga gcctgggcga 420gcgcatcaac accttggtga ggtacctgct gcaccgcgcc aagaaggggc tgggcatgcg 480gcgcgccgac gtgtccatgg ccaacatggt gctcatcggc ttcttctcgt gcatcagcac 540gctgtgcatc ggcgccgccg ccttctccca ctacgagcac tggaccttct tccaggccta 600ctactactgc ttcatcaccc tcaccaccat cggcttcggc gactacgtgg cgctgcagaa 660ggaccaggcc ctgcagacgc agccgcagta cgtggccttc agcttcgtct acatccttac 720gggcctcacg gtcatcggcg ccttcctcaa cctcgtggtg ctgcgcttca tgaccatgaa 780cgccgaggac gagaagcgcg acgccgagca ccgcgcgctg ctcacgcgca acgggcaggc 840gggcggcggc ggagggggtg gcagcgcgca cactacggac accgcctcat ccacggcggc 900agcgggcggc ggcggcttcc gcaacgtcta cgcggaggtg ctgcacttcc agtccatgtg 960ctcgtgcctg tggtacaaga gccgcgagaa gctgcagtac tccatcccca tgatcatccc 1020gcgggacctc tccacgtccg acacgtgcgt ggagcagagc cactcgtcgc cgggaggggg 1080cggccgctac agcgacacgc cctcgcgacg ctgcctgtgc agcggggcgc cacgctccgc 1140catcagctcg gtgtccacgg gtctgcacag cctgtccacc ttccgcggcc tcatgaagcg 1200caggagctcc gtgtgactgc cccgagggac ctggagcacc tgggggcgcg ggcgggggac 1260ccctgctggg aggccaggag actgcccctg ctgccttctg cccagtggga ccccgcacaa 1320catccctcac cactctcccc cagcaccccc atctccgact gtgcctgctt gcaccagccg 1380gcaggaggcc gggctctgag gacccctggg gcccccatcg gagccctgca aattccgaga 1440aatgtgaaac ttggtggggt cagggaggaa aggcagaagc tgggagcctc ccttcccttt 1500gaaaatctaa gaagctccca gtcctcagag accctgctgg tacccagact a 1551203936DNAHomo sapiensmisc_featureIncyte Clone No 1236935 203gtcagtttag tgtttgtggc attcccgctg cttttatggc acttcagtca ttttttagca 60cacttccatc catactacat gtgtcctttt ttccccttaa cttctctaat tgtgtttctt 120atccttttct ttaaaaccat tgcttcatct gggagtggtg gttcatgcct tggcctccca 180aagtgctggg attacaggcg tgagcaccgc gcccggccaa ctatagtgtt ttcaaaacat 240gtgtacacat actctatgag gatgcaaatt gagatttcaa caaatatttc tcagtgactt 300acataaagcc gtgctttatc ttggcgctta gatgaatttt gtttggttgg ttttggtttt 360ggttttacat atatcctagg aacatagcag gtgatataga gtggtaaaga gcacacgtcc 420actgttagta ggtattttta tgcacttgtt ttctcatcta taaaataagg ataaaattag 480tgcctacctc acaggatatt agggagatgg agagaatgct cagaacacaa cagggcctag 540cacagaggaa gcacaatgct gaggaacgag aaactgcacc tgtaaattct gcagtcactt 600taaattataa aacgagtatt tgatgtatga tcataacttt gctaagaagc catcagttat 660aatggatgca tgaactgtag ccatccagtg agtagtgacc aggatggagg agctttatgg 720agggggaaga aaggaacctc aaagctttcc gattcatttt gaatcatgag atgtctacat 780gtaaaaattc tgccttggta aactttgttt ataatgtttt agataatgca ttcacatggt 840tcagatgtat gaatgtgata tattagttat ttgtttataa atatatattt tataaacata 900tttataaata tataaatata tatttgggga acatat 936204432DNAHomo sapiensmisc_featureIncyte Clone No 1359283 204cgctgtcctg ctaaagcaga ggatcacagc tttaataaag accctaaata tttatcttgc 60ctgtggtcat gtatagctga acaatgcaca gcgaactcat ttagtttcca tgcgcttaac 120tgggctaact ctgcttttga gcctaatgga aagcttgggg caggtggagg accggttctt 180tagcactcac agacgattcc cacaccacac tcccatatcc ggtcttctct gccgagaatt 240ctccctgccc aagaggtctg gggtgccctg gacacgtgtg ctcatctcct gtatttggag 300atctggggct gggaagagaa tgtaaagcaa cctaaacagt aatttaagaa tggagaaaat 360gggactaaat tattcagaca cgtttgagtg cctactcgct agcaggcatt ttccgctgcc 420tataattatg ag 432205971DNAHomo sapiensmisc_featureIncyte Clone No 1450703 205gggagagagg ataaatagca gcgtggcttc cctggctcct ctctgcatcc ttcccgacct 60tcccagcaat atgcatcttg cacgtctggt cggctcctgc tccctccttc tgctactggg 120ggccctgtct ggatgggcgg ccagcgatga ccccattgag aaggtcattg aagggatcaa 180ccgagggctg agcaatgcag agagagaggt gggcaaggcc ctggatggca tcaacagtgg 240aatcacgcat gccggaaggg aagtggagaa ggttttcaac ggacttagca acatggggag 300ccacaccggc aaggagttgg acaaaggcgt ccaggggctc aaccacggca tggacaaggt 360tgcccatgag atcaaccatg gtattggaca agcaggaaag gaagcagaga agcttggcca 420tggggtcaac aacgctgctg gacaggccgg gaaggaagca gacaaagcgg tccaagggtt 480ccacactggg gtccaccagg ctgggaagga agcagagaaa cttggccaag gggtcaacca 540tgctgctgac caggctggaa aggaagtgga gaagcttggc caaggtgccc accatgctgc 600tggccaggcc gggaaggagc tgcagaatgc tcataatggg gtcaaccaag ccagcaagga 660ggccaaccag ctgctgaatg gcaaccatca aagcggatct tccagccatc aaggaggggc 720cacaaccacg ccgttagcct ctggggcctc ggtcaacacg cctttcatca accttcccgc 780cctgtggagg agcgtcgcca acatcatgcc ctaaactggc atccggcctt gctgggagaa 840taatgtcgcc gttgtcacat cagctgacat gacctggagg ggttgggggt gggggacagg 900tttctgaaat ccctgaaggg ggttgtactg ggatttgtga ataaacttga tacactaaaa 960aaaaaaaaaa a

9712061832DNAHomo sapiensmisc_featureIncyte Clone No 1910668 206cccagtttta tctgctcctg agactgagcc cagatcccca aatctaatct gatttacagt 60tcaaggaagc tgatggggag ctgggcctta cccctgatgt aggaggggca cacagctggg 120ggtgcagagc ccacctgggt acctgacccc caggggatga aaatgcaagg gtgagtctgc 180ttgggcctga gagtttgatc tgcaggggca ggctcatctt ttctctcccc tgccttctcc 240tccttctctc cccagagccc ccttgagccc ctctgcctat gtccctctgc ctcctcccca 300tgcccccagt tgctgtggct tgattctgct accctgaccc caccatgtgc caggtggcat 360ctgccttact gccttccctg aggagctggg acatgctggg cagttgtcag atgtaaaggc 420acagctggag cagagggcat gtcagtaatg attggtccct ggggaaggtc tggctggctc 480cagcacagtg aggcatttag gtatctctcg gtgaccgttg gattcctgga agcagtagct 540gttctgtttg gatctggtag gacagggctc agagggctag gcacggaggg aaggtcagag 600gagaaggcag gcagggccca gtgagagggg agcatgcctt cccccaccct ggcttgctct 660tggtcacagg gcggttctgg gcacttgaac tcagggccga agcagaagca caggcccagt 720cctggctgca agcacaatag cctgaatggg atttcaggtt aggcagggtg ggaggggagg 780ctctctggct ttagttttgt tttgttttcc aaatcaaggt aacttgctcc cttctgccta 840caggccttgg tcttggcttg tcctcaccca gtcggaactc cctaccactt tcaggagagt 900ggttttaggc ccgtggggct gttctgttcc aagcagtgtg agaacatggc tggtagaggc 960tctagctgtg tgcggggcct gaaggggagt gggttctcgc ccaaagagca tctgcccatt 1020tcccaccttc ccttctccca ccagaagctt gcctgagctg tttggacaaa aatccaaacc 1080ccacttggct actctggcct ggcttcagct tggaacccaa tacctaggct tacaggccat 1140cctgagccag gggcctctgg aaattctctt cctgatggtc ctttaggttt gggcacaaaa 1200tataattgcc tctcccctct cccattttct ctcttgggag caatggtcac agtccctggt 1260acctgaaaag gtacctaggt ctaggccctt cttccctttc ccttcctctc ccctacccca 1320gaactttggc tccctttccc ttctctctct ggtagctcca ggaggcctgt gatccagctc 1380cctgcctagc atccatgacc tgttggatgt tacctccaat cagtttcctg tcctacctgc 1440ctctttggct tggacctata tggccatgct ctggctctac ccttgggaag cctgatcccg 1500gtgtgtggcc cagcttgttc aggccctggg atgctgcatc tccaggcaac tatgcacttt 1560cccggggaga gaaccagtat gagaagtggg ggcagggcac acattcatct ttgtaggaag 1620gtctggcctg gggtcgggtg aaggagggcc caggtcagtt ctggggtccc agtgacctgc 1680tttgccattc tcctggtgcc gctgctgctc cctgtttctg gagctggatg ttccccagct 1740ggcagttgag ctgcctgagc caatgtgtct gtctttggta actgagtgaa ccataataaa 1800ggggaacatt tggccctgtg aaaaaaaaaa aa 1832207567DNAHomo sapiensmisc_featureIncyte Clone No 1955143 207catagatcca taaatcatca tctgttgcaa aaaagcacat taattgattg gttgaatggg 60gagattgaga tatttctttt ctcttcttct gctcaggtgg gggcaacttt tgggggcaga 120tgagttctgt tgccacaaaa gttatatagc acatttggtt tgcactgaat cagcgattct 180caatcctggc catgctttag aattatacaa gaaaaatctt caagtatcaa tactcagtcc 240ctatcctact gatccaattc atctatgata aagccagagc attgattttt aagttctgca 300agtgattcta atatacagcc aaggctaaga actactgata tgttccaaac actcctattt 360tggagataaa gaagttgagg ctgaggatga gaccttagca cataaagttc cataactagt 420aacagaccga agttctgtcc ttacaaataa aaaaaaaaag gggcggccgc cgacttagtg 480gagcttcgtc ggccccggga atttatttcc cggaccggta ccttgcaggg ggttccaagc 540ttttcactct atagtggagg ccgtatt 5672081303DNAHomo sapiensmisc_featureIncyte Clone No 1961637 208gggggacaat tccacccact cgagggctgc cccctcttcc ttagcagacg aaccagtaat 60gggggcaagg ctggggcatc ccagcccaca caccctggat gcccagcaag gccacagaaa 120gagcctgatg tccatgatcc aggtggctct gagaagcttg gcctggacac ctgagcctgc 180ggccggtact cctgccttct ccccatctat ccccaaggcc tctgcctctc agcctcttcc 240atggtcggtt taggctgctg agttttctgt gcttccccaa gaaccagtgg gatcaatgcc 300ggcggcctct gtgatggttg ctgactaatc cgggatttca tgagtcagag gcaccacccc 360tcaccccagc tgcctgctgc ttctgacgga tcttggtgct caggctgcct ggctctccga 420gtgaggacgc agcctccata tttggtgcac tcaggcatgg ctgggacaag ccagctgccc 480cagggttctt cccctggtga ttctcgcctg ctttctcatc tcaggggagg cagtggcacc 540tccctctccc tgctgacatg aagagagcta tgatatgcca ctgctgccaa ctcatcctct 600gcccccacct cgaaacccac agtccccagt ggagggccac tactcatccc cattggtttc 660ccaggggagg ggtgttgtct ggaagggcag gttcagatgc agccttccag atttagaggc 720actgggagga cagtggctga gtggaggcgc ccagacctgg gcaggcagca ggctcaggcc 780cacaccttgt gatttttgaa accaaagccc agaagatgat gtttacttct ctctccctgg 840ctctgccctt cttactgcaa accatgctgt gccttagggc ccttctcata gctgttcctc 900atggccatga ctggaacagg gatgcaacct ctttctacac aagcacagtt agttgggtga 960agtctttttt tttgtttgtt ttagacggag tttcactctt gttgcccagg ctggagtgaa 1020gtggcgtgac cttggctcac tgcaacctcc aggccagcct cagcctccct agtagctggg 1080actacaggca cccactacca cgcctggcta attctttgta tttttagtag agatggggtt 1140tgaccgtgtt agccaggatg gtctcgatct cctgacctcg tgatccaccc acctcggcct 1200cccaaagtgc tgggattata ggtgtgagcc accgcgccgg gccggttgct ggcatcttaa 1260tgttctgtag gtggaatatt tccaataaac acaaggtcgc cac 13032091355DNAHomo sapiensmisc_featureIncyte Clone No 1990762 209gcagctcttt tctggaaatt tctcagggga tgtatctgaa gctccaggtc tctgagatac 60tctggagggc ccgaccttgt ggagatgtgg ccaaccacat gggcctggag ctgggttcaa 120accctgactt tggcactact tattagctgt gtgaccttgg gacagttaat taccactctg 180caggtcagtt tcctcatctg tgagatggat gtaataatag ggtgtgatga gatgatccct 240agtgagtcat tggtgttgct gtggccacca ccattgttgt tactgggaga gttttggatt 300tggaatcccg tgagcaggat tctattctgg ctgtgccatg tgccagctgg gcagctgtag 360gaagtcactt cctctctgag ccttcacttc ccagtctcta aactggggct cacaaatgtc 420gcattgcagt ttgggggtgg atcttttgta agaatggaca gaaaaaagat ggtcaactgt 480aatgtgtggt gcatcgtgag ctgtcactcc cgtgtgccct ggtctcctgc tggcctcact 540gtggtttgac tcagacttgg actttcctgg aattctgaac tttgcctctc taagcaaagc 600cccgccaggg ggtacactct gccttgtttt ccatggtgcc gtgtttccag ccctatccaa 660caactggctt ctgatcggct gctttttcac actgctgttc ctgcaaggct gtgtggcccc 720atggttaagg gtgagggttc tgcaagggtc agccagatgc gagttccggt cctaggtcca 780ccacttactg gtcaggtgac ctcaagtaag ttgcctaacc aaggcttaac ctcttaggag 840ctcagttttt cttcctgtaa aatggggata ataatagtac ctacctcagg ggaatagggg 900atgaaaaatg gtcttatgaa atccccctgg ccctaactgg caaaagccaa ctcagttaac 960ggggctccat tatcactgtt gggacctggg cttgtgggag ctcaggagtc ttctcagacc 1020tcctcattgc tgtgccaggt ggaggaggtg tttgtattta ctgagagcaa ttgggccaat 1080ggcccatagt ccttgagcac ccagctgacc caggccacag aggctgctca tcttggtctg 1140gtgaccacag gaggctgtgg ctgttgggat gaccctcccc agtgttgtta acaacagtcc 1200caggccatgt cctgctggcc ttgagttccc ctgtcctctt gtgaatgtcc ctagagccat 1260ggcctcaagg ttcctgaagt tcccaataat gtgacatgct gcccagacct cactacactc 1320cttttttatt ttgagcctgg gtgacagagc aagac 1355210776DNAHomo sapiensmisc_featureIncyte Clone No 1994131 210gttcactgcc atattcctag ggacaagtaa agttccctgt atattgtagg agctaagtgt 60tgaatgaatg aatggtggct gctattattg cttcaccttc accctccaag agtaatctcc 120ccattctggt ttatagtttc tgtgctcact gcttgtgata atcgtaagta tatattgttg 180agaacagtgc ctgttttctc tttccctgaa aacacatact ttgacgttgg ctgacatagt 240tcactcagct gttcctaacc actgatccct ctgtatcaca ggtatctcgg gggagctttg 300tgccttgatg gatcaagttc atcatatgca gcactcaaaa tggcagcatc cttcggacct 360caccacgcga aactacgccc gccgacagaa acatctgcaa agatacagtc tgactcagtg 420ggttgacagg aacatgcgaa gccaccatcg gttccagcgt ctcccagact tctcgtacag 480ttaatttgtg tcatcccatc agcaatgaag gtccctatcc agggtcctgc ttggagcagc 540atttcatgtt cttttgctgt tttgtgcttt gccgattttg gattttattt ttcacaaaat 600ttttatttaa aaaactcgtc accttttgga aatgcccatt gccgacttga atttttttgt 660atggagtccc cctgattttg tgtgtgtgtg tctgtgttta agcacgcgtt cggttggtat 720agttttttat atgtattttt acattaaatt gaaggtagct gcctcctgga aagcag 776211817DNAHomo sapiensmisc_featureIncyte Clone No 1997745 211ggaggcgtta gaggagctgc cttcggaggc tcagggagtc cctttggagc tggttgtttc 60cttggccctg cagcgcactg ctcggggctc ccaaggaggt tgtgtgtatg gttcttaatt 120catcaggaca aagaccccca gcatgtgtgt accctgggac ccgatttctc tgggcccaca 180tctatctcca atacctcagc ctcagatcag accctttctt ttttgtcttt cttctcttaa 240tttttaaatg cctcttttct tgagcattcc atctctcttt ttgaccctct caggactggg 300cttagctgtc cagagccctg ccggagggtg ctgggggctg tccctctgca ggcactgtgt 360tttcctcagg ggctgtcctc agaacacccc tcctgctccc tggggctcct cagggagcca 420tttcagctgg agtctcaggt ctcaaaaaca acttctccag gaggccaaaa aaagactggg 480ttggcttctg gtcctcatga tggcttttat cctcctggga cactttgggt atattcatgg 540gcattgtttc catctgtctt ttctacctgt gccacccctg ccctgattcc acggctgcct 600caggcaggca ggcaaggagc taggccggtg cccggccctg gcagcaaggg gtctttgtgc 660agttggagat gctgccgttg tggcagagcg tcctgcagcc ccgcttccat cagcaggctc 720tggggtgggg gctttgcagg ggatgctctc tgatgtttgt tccgttgttt aaataaaatg 780cacttatttt tgtttttttt tttgcaaaaa aaaaaaa 817212484DNAHomo sapiensmisc_featureIncyte Clone No 2009035 212ttttctttta tatgtattat taaatgactg ttactctaca aacatatggt tgttattttt 60actttttgga taccattata gtgtaggcat ttcccaggtt tttttggtaa caccattttc 120ttaatgatat gatgttgcag ccagtggatt tattacagtc ttacttatta ttgctctact 180gttggtcctt tagtttgctt ttcactcttc tatgtaatgc tgtaagaaat gactttttcc 240ataaactatt ttccatatat tggatgtata atttaacaca ttctaaacat taatgttaaa 300acagacataa agcataaaaa ccgagatata tatttgatca tataaaaatt taagctgggc 360acagtggctc ataccctgta tcccagcact ttgggaggcc aaggtggggg tagactggtt 420gagctcaggg gttcaagacc agcctgggaa catggtgaaa cccaactcta ccaaaaaaaa 480aaaa 484213509DNAHomo sapiensmisc_featureIncyte Clone No 2009152 213cccagtttat taccattaga ccataccttt ttgtccaatc atttaaaaca aatttttata 60taataagttt tatttgtatg taataaattt tattatataa aaataagttt taatatatat 120tatataaaaa gttttaataa atacctaata tattatttaa tatgataaaa cttatattaa 180atgaaatttt atgctgttct cttgtcaatc tgtcttttgt tatcttgctg gtgtgcctgt 240catgtgaggg actgcaatct gatatgccta ttttccacag tcaaagcaat tacaagagaa 300ttgttacaat tacccagtta tgtcaagaga ttttttttta attcactaag gtagagataa 360ggagaatgta ttaaaatagg atattttaat tataaatgca tgactgggga gggggtattg 420tttttgaata aaatatgagg ttatttgcca tgacaaaaaa aaaaagaagt aggaaaatcc 480catggaaatt tatgttcctt ctaactttt 5092141130DNAHomo sapiensmisc_featureIncyte Clone No 2061752 214ggatttatca cattctgcct tgaatcatag ggaacagcat gtgtagtgga atgaacacag 60gcctctgaat ccaagatatg agtttaaatc ccagctttgg aggtggttac ttaaagtctc 120agtgccttca ttcttcttcc tatataaagt agatattaca atatctaact tacagagtca 180ttgggagcta tacatgcagc gattgggtaa agcacctggc acatggcaag cgattagcaa 240atgctggtta cttctacttc tttctcttcc cttttcccag tctatcataa tttccttgag 300agcaggcacc atgtcttatt tacccttgta tttcccacag tacttcccat agtgagttac 360ccttagtaaa tactcagtaa gttgaattga atttaaatta cctgtaagtc ttaaaatgtg 420ggattaaatt aagaatatat tgtcctggaa atacccaaat gtctattgat ggatgaatgg 480ataaacaaaa tgtggtatac acataatgga atattattca gccttaaaaa ggaatgaaat 540tctgacatgt gctacaatat gatgaacctg gaagacatta tatgtgaaat aagccagaca 600gaaaaggaca aatactatat gattccactt atatgaagta cctagagtag tgtaattcat 660agaaacagaa agtacaggtt gacatccaaa atctgaaatg agaaatgctc caaaaactga 720aactttttca atgccgacac gatgctcaaa gaaaatgcta attggagcat ttcagatttt 780ggatttttgg atttgggatg ctcaactggc ataatgtgaa tattccaaac tctgaaaaaa 840tctgaagtct aaaacacttc tggtctcaag gattttggat aaaggatact caatgtgcaa 900catgtagaat ggtggttgca aggtgggagg agagaatgga gagttactgt ttaatgatac 960aatgtttccg tttgggaaga tggaaagttt tggagatgtg tgatggttat ggttgcgcaa 1020caatgggaag gtacttagta ctgcttaact gtgcacactt aaaaatggta aaaatgataa 1080attttgtgta tgtcttaaaa caataaaaga agttttttaa aaaaaaaaaa 11302151273DNAHomo sapiensmisc_featureIncyte Clone No 2061933 215attttctccc ttcagcaagc actcattaag gagtgaggct gagtatttta agatagagtg 60agatctgtga gtgattgaaa ggtgatattt aaaaacttgg atttcattcc agtgtcaggt 120ttgggtttta agttcctttg gtccagggaa gggtccaagc agccacagtt gccctaaatc 180tccatcatta agtcttccag caaggttaag tgcagtatgg aaggagaagg gggaagagga 240cggtaacggc cccacactcc aggctgagaa agagtaatta ggaggcctga ggaggggccg 300aggaaaggct gttggggtgt gctggggttg gtacccgagc gccttcccct cacctcaacc 360agagaagagc atccggttgc tttttaaagc ttttagcctg ccctagcaag gacaaagcat 420gttagattag agatgcttct gctgatcgca ggggttctta tttgaaaaca tctatgatgg 480gggtggggtg ggaggagaca ggttgtggtt atgcaggaaa atcttgtcct aaaaatatat 540gagtttgggg gtaaggggtg ggatagccaa gcaaaatcag taattatttt aaaatgaaca 600tatgtatttt tattaacttt tagttaaata cagattttac aacgaggtca gcataagcct 660aaatctatat agagggctaa ctcaggcatt gtcttgttta tttgtagact ggattaaaaa 720caacctgtcc tgttttgtca gttcccagct tcttcgttta gaataaatta gaccaaaaga 780agaaacgtgc ttgtctctgt atacccgcag aatgaagtta ctgttgttaa aactggattt 840tttcatttta ctaggttccg aagagtccag atgcttggta gatgttcaat acgtgatttt 900ttttttaatt gaatgtgttc atttaaaatc ctccttaaca tttctagaaa gacttctttc 960aataaataat ggaatcttag aggaaaagtg gttttttaaa agctagggaa ctcctccact 1020aaaagtaacc attggaaacc tcgaatgagg gctaaagttt taatcataag agaaaaggca 1080gcataatgaa atgtgtacac atacatagtc agtggtccat tttaggaagc cagtggcgtc 1140tgataaagaa atgttaagag tagtgaggtt gaggaaggaa attgtgggga tttgaaatat 1200tctctttatg ttgtttctct tctgagtcat ggtaaaacaa taaattatca tctctaggtg 1260gaaaaaaaaa aaa 12732161279DNAHomo sapiensmisc_featureIncyte Clone No 2081422 216ctttaacaga aggatggggg atagtcagat agtcggagga agtgggtgat ttgaacaagt 60tcagagcagg gaggaaggct gtgttgggga cttccagctt gctctcctca tgcatgaagc 120cactcattcc ctttctctct cccccacccc ttcttcccct cactttcttc ctttcctcac 180ttctcctttc ccctctgtgc agagctcttg gcaccagtca agctgtccca cccctcaggg 240ccctctcagt gactgatgcc catggctccc tcctcctaca cccaaagacc ctggcttgcc 300catgtctctg atgagaattc aaagggagct gtgtttatat aacgtagagg gatttacctg 360tggcttttcc tttactcact tcctcaaaac tgtacattta tggcatagga tgtcagtcct 420aaaagtttta ttatcaaaac agtaggtggc aagtaattat tatcataaat ccagcaggtt 480ctagagaagc caagttggag gagaaagcag gatagagtcc accatgacca ttgattgttg 540ggcacattct ttctaagaaa cagattaatt ccattgtatc tgttctctgt tatcccatac 600cagcttatga ttagagtctt gagctcacaa cttggtcctc taagaggtag tcagtggtca 660gcgcttcagc ttgaccacag cgtttggttc tttctttaag tgttgtgttg taatgcttgg 720attataaaag ccttaacacg gccccatttg atcagttccc tgccaactct tgtatcctca 780tttcactaag ctttgttaca ctcactagac tgttaacaac ggagaaaaac ctgtgggtac 840tgaatatgcc atatacaact tgctatttat tctgttccct gtttagaagg ccatggctac 900ccttaactat ctgaactctt cctgtcctgt aagactgagc tcactggcaa tatcctatag 960gctgctttcc ctaagcctcc ccatctttct tcctccctcc ttctacttct ctcctacctc 1020cttttccctc tctcccctac tcacctgctt tccttttgcc cctcccacat cctcttcccc 1080cttcttgtca tttttccatg tcaagaaatt tccagatata taggaatatg atggagaatg 1140ctgacaggca gttctttgag tagtcaaatt aagatgtaat ggttgaattg tataatggca 1200atcacataaa ctacatatat aaagcttcta gcttagtaaa ctctaaatgt gtttttttaa 1260actaaagaat gaggggggg 1279217899DNAHomo sapiensmisc_featureIncyte Clone No 2101278 217tggtttggga atgcttcgaa ttttattttt tctactccca attaatcagg agttgatgat 60cccatgagca ggaccgcctc catgattggg gagcatgcac ttgtgactgc agggtaagag 120tgggaagata ggtttgtgga gtggcaccga caggactgtg attgtgtgtg ggcctgcccc 180acatttctct gggggatgct tatgtgagag tgggcccagt gaaagagtta ccaagccacc 240cacaccccta acactgttct ggatgagaga tgagagcaga ccggcttctc cccatcagtg 300cattgtgcct gttgtacacc cctggaggag ccctggagcc agcccaggtg gggtacacaa 360tctttttaaa ttccatatgg ttgccagctt atttctttca cttgtttact gtaatatctg 420gcgtgttttt atttatctaa ttttgtattc agttataacc atggtagggg tagtgaatat 480atgacaggtg taatccctgg tgctgcagtg gaccttcttt tcttttggac aagataatac 540tgtgagtttc cctccttcct tccctctaat ttgttttcct tttttcccca gcctcttgca 600tccccttctt ttctaccctg tcctacaact atcatatgca cagtcttctc tctttgtgtg 660tgactgttac aaaatttcac ttttcaaaat cgaaatcagg tgtttgctca aatgagggga 720gatttttttt tttttttttt ttttaaatgc tgagacttca gcagagtact ttccttttgg 780tggtttcccc caaaaaccca tcagtctggg agagcattgg gagtggaaat catgttgcct 840gggatgctgg tttctttgaa aattatataa aacgtatgta aaaggtcccc ccatttggg 899218645DNAHomo sapiensmisc_featureIncyte Clone No 2121353 218caaagtgctg ggattacagg tatgagccac cgcaccgggc ctgttctatt tttctagtta 60agggaactga agctcagaga ggtgtcacca gcaggtgttc attcccatgc cagccttgcc 120ccccggcttt tcccaggcag gctcctgcgt gcccactggc tccagcctgg tcctctgtct 180cttggctgct tcactcctgc tctttgtccc gactctggcc ctgcttacag gggccactac 240ctgctggtgc ctccataaca agcgtctggc gttgagaccc ctggcatggc aggggctttg 300gggtctggtt tccacaaggc ttagccatgg cagaacctcg ttttatttta actctttgcc 360cctacaaaca aacagcagta cttgccagaa ccattcttgg gattcaggag ctcgggcgac 420tgccttggcc tctggccgca cccaggaggg tggggttgga tctgtgtagt tgccaggccc 480acacctgcca gcagggggct gactggatcc atgctttact gtgtttaatg ggggtaacag 540gggtccctac agccctccca gctaaacatt tggaacaaaa caccagccct tttgtagtgg 600atgcagaata aaattgttaa tccaatcacc tccaaaaaaa aaaaa 645219703DNAHomo sapiensmisc_featureIncyte Clone No 2241736 219ccacgcgtcc gctgtaaacc agaaaaatgt tggttatcta gaaaacttga gagagcatgt 60agattaactt ttctctttgg agttctaaaa cattaactgg aaagattaga taatatacta 120aatgtataca gaagtataca gactatacaa agactgaaac aagtcccttt tgcactacaa 180ctctataaca ttaccgcaga aattttggtt ctatgtagca tggacctcct aaggaattct 240gtttctttta gcattgagat ccctggtgct ctttttttac ctcagaattg gtacaatcat 300tattaaacgt taatttattt caaacttttt aattgaaaaa aggaaaggga aacttaattg 360gggataaatt caggcatcat attattatga tagagtctcc tgagtggttc gtctataggt 420aatgaactca ttggtgttat ttcttggaca tcttggcctt ttaatcaaag actgtgtgct 480gctatttgct atgagcaagg tttctcaaaa gcaaaaggtg cttggaccat ttggatcacc 540tgagttagaa tctctaggta tagggcccag gtatctgcat tttcacaggt ttcttgtagg 600tgactttctg caagctaaag tatgagaacc attggcttgg atgtagttct aaacttttag 660gtctgtaaat cttgaaatct tgaactgaag gtcaactatt ggc 703220536DNAHomo sapiensmisc_featureIncyte Clone No 2271935 220ctttcatcat aattaaagtg ctgtcaggga aaatggcatg gctgagtttt gctgctgttg 60aaatgaccct cctcctccac tcctcttcgc ttctctcatt tgctaaagtg gtcctttctc 120tgcctgaaat caggcccttt ggtgatggaa attttagctt aaagcagagt tctaagcaga 180atcctaaccc tgcgagggtg

gggagaaaat caatgttttg agctggtgtc tgtttgcagc 240gaggtgctgg tgaggccatt ttcatcagga ggaacggtgg tggtggctac ttctgggctt 300tagatccacg caaggtctcc taaatacaag tcactgtcat ggtacacaat ttagcaaaac 360ttggaggctg attttccccg ttgacttagc tagggtcagg aggaagctgt ttagaagtac 420agaggttctg catctgggag ggtaaaatcc aaacgcctct catgctcaga gggaaagcat 480gcctgcatgt ttactatcac tgctggccta cgtgcttgtg tgctgaattt agatgg 536221790DNAHomo sapiensmisc_featureIncyte Clone No 2295344 221tccgtccccg gccggtagat tttcttctct ttctaaggct aatggtggta gttttgtttt 60ttgacgtttt cttataatga gtttttcttt ataattttta atttatgctg taatgtttct 120tatttacaat gttatctctt aaatctttga gtacattaca ttttctcccc tgataatctc 180ttctaaatta ccttctctag ttggttttct tcccttcctt aatgttagcc attcttcagg 240tgaaggttaa tcctcaatgt actcttcatg tttaagggga gggtctaaaa ccttgtgggt 300aggacttacc aacggagttt cattgcatga tgatcttatt gagcttattg gtagccctta 360tctcagtatc tttagttttt cttgggctgg tcagattttc aagagaagac ttttcatttc 420ctttgtggag ggaaaaggcc ttttaccagc actcttcaag ctcagtaggg gaaagacttc 480aagcactcag gaagcatgca ttcactttat ttggaacaat acccttactt gtaactgtgc 540ctcaggtgcc atagtccaca gagacttctt ttacctgtcc agagaataaa attagttgtc 600tgttggggta acaaaaagtg tggagctgaa gagggtacct ataaatgaag ttgttttctg 660gccgggcgca gtggctcacg cctgtaatcc cagcacttcg ggaggccaag gtggagggat 720cacttgagtc caggagtttg agaccagcct gggcaacata ctgagactcc gtctctccaa 780aaaaaaaaaa 7902221045DNAHomo sapiensmisc_featureIncyte Clone No 2303994 222gggaagttga ggctgcagtg aactatgatt ttaccactgc actccagctt gggcaacaag 60atgagaccct gtctcaaaaa aaaaaaaaag ttttctagaa taagcaggat gattgtttaa 120tttgaagatg gaacaggaaa ctagagtgca tttaaaatac tctgtcttca ttttaacatg 180ttgaatggaa taactgcata tcaccatgag tttgttttgc ttttcataca gacttgtatg 240tgtcatttga gtggtttcca gattggagcg aggttattct gatctaaatg aacagcattt 300ttttccttag cctctgtttg ccactctggg tatctctcct atgggcaaag ccattagaaa 360tgcataaaac ctcgagacat ggtttttggc aaaaactcca tgactttaaa ctagctcttt 420tactactgac ctttcacaga gaaaaaatat ttcccttgaa aaaaactggg cttgtcattt 480tttcccttgt agctttaagc agagacataa gtgccttgca ttacacatag taaactttct 540ttaaaaaaaa aaaaaaagat tttggagact accagggtaa gattccaact tgtccaaaag 600ctttctggcc ttacatattt tattataaaa attctcaagt ctggtaatct tctatgtcag 660agctagtgat ttcaaaaggt ttcacaattc cccaagacaa aagtgatttt cgttcattat 720aataaggtta agtgatatgt gattcataac aattttgatg tgaagaaggg aaggacatca 780ttgacttaat aatagtatca gtcggtgcaa cagttggcaa catgtgcctt cacactttac 840cataaagaga cgggtttgag ggtttgcctt ctaaagtctg caacttcaag aaaaaaaatc 900gacaccgtgg attgaccttc ccgggtccac taatataaag ccaataaagc ttaaaaacac 960ctttggtaac ccatgtaatt taactccggt ccagtggccc tataattcca attaaaaatg 1020gttcaatctc ttggaaaaaa aaaaa 1045223553DNAHomo sapiensmisc_featureIncyte Clone No 2497805 223ctggcagatc cggacgggca ggactgggtg tgtcccatga gagcacctcc ttcctggcct 60ttcctgtgga ctttgtccca caccacctgc ctgggttcct tcctttagtc acttccagct 120ccaggcacag cagttggtga ctccttggtg ggagccgtgt cccacccggt cctgatactg 180ccgtcttctc tttcacagtc ctccaggctt gggccagcct tgggggcagc agagcttctg 240gggtgagtgt cgagatcctg tgtcctgaga gcggtagtca gggagagggc tggtcggggc 300agggctgccc gggcaggaca caggatgcgg ccggccaggc tggggccaag gtgttcagac 360ctggactttg ggctcgtgct ttcttcatgg ttgcgccttg ctcgctgtcc cttggagtct 420tcatttggtt ttgctttttt tgtttgtttg ttttcaccta atttttgcca gacttaagct 480agttttgctg ccttttgaaa ctagtggaag aatcatttta ttcctgggga taatttgggg 540gcttttgaat cca 553224706DNAHomo sapiensmisc_featureIncyte Clone No 2646362 224ccgaccccca acctcaagtt gccgccggaa gagcggctca tctgaacgct ggggcctgct 60gcagccacca acactgccca ggactgcggg ttgctggctt gtacaccgca gctgccaccg 120agacaccagc ctctgatggc tcaggaggac ttgtggggag aggctggggg cacccatgtg 180gtgggctctg tgcagcatgt tgcctctgct tggctgtgcc tgcagctcag ggtgctgggg 240ctcgggaccc acccccctgc ttgcggaacc aacttttctc tgtgtgtcca gcaggcccca 300caaccccctc tcctttcttt cagttctccc atgcagccga ggcccgggcc cctcaggact 360ccaaggagac ggtgcagggc tgcctgccca tctaggtccc ctctcctgca tctgtctccc 420ttcattgctg tgtgaccttg gggaaaggca gtgccctctc tgggcagtca gatccaccca 480gtgcttaata gcagggaaga aggtacttca aagactctgc ccctgaggtc aagagaggat 540ggggctattc acttttatat atttatataa aattagtagt gagatgtaac aaaagcttta 600ttggtgtgtt tgagctggtg ggtgccacat atttggggat ttgaagaagg aggtgagatg 660tctggatggg gactgggatg ggtagaggat tcagtgatac tccgag 706225509DNAHomo sapiensmodified_base(492)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 2657146 225aaattttagt gtattacatt tgcctttact gtttatgtgc agcataaagt tgcttttgtt 60acaattcatg ttgttttgta atggttgatc aaagcaaaga aagacatgtg ttactacgca 120tgatctgtca atgtttaagg ctgttgttgg ttcttgtgac tttgctaata tgtttttctc 180ctgacaggtt aacctgccct cttaactcag cagtggttct agcgtcctat gccgtacaat 240gtaagtcaca aagggagcat ttcacggatg gacaggttgt tctgatcagt gtgtggagaa 300agtcactggt tcctcctgct tgaccaagtc cctcttcccc aggaatcctg ctgggcagca 360tatctctggc tgtccagata tgtgtttcta ctcagactgg cactctcctg tagcatgggg 420atgttagatt aaggaaggtg gttaaagggg aaagaatgaa tgaactgtgg tgtgaaattt 480cttccaagga gnccatccga cagcagaca 5092262153DNAHomo sapiensmisc_featureIncyte Clone No 2755786 226gaaggcggtg gctgaggcgg ttccggaggt tctagtgtcg gagttgggtg caggcaggtg 60ccatgggccc gcttgaggca cactgagggg acgcggggct gggccatggc cggcgctcgg 120gccgccgccg ccgctgcctc ggcggggtcc tcggcctctt caggcaacca gccgcctcag 180gagctggggc ttggggagct gctggaggag ttctcccgga ctcagtaccg ggccaaggat 240ggcagcggga ccggcggctc taaggttgag cgcattgaga agagatgtct ggagctgttt 300ggccgagact actgtttcag cgtgattcca aacacgaatg gggatatctg tggccactat 360ccccggcaca tcgtgttcct ggagtatgag agttctgaga aggagaaaga cacgtttgag 420agtaccgtac aggtgagcaa gttgcaagac ctcatccacc gcagcaagat ggcccggtgc 480agaggacggt ttgtctgccc agtaatcctg ttcaagggca agcacatttg caggtcggcc 540acactggctg gatggggaga gctgtatgga cgctcaggct acaactattt tttctcaggg 600ggtgcagatg atgcctgggc agatgtggag gacgtcacgg aggaggactg tgctcttcga 660agtggtgaca cgcatctttt tgataaggtc agaggctatg acatcaagct gcttcgatac 720ctgtcagtca aatacatctg tgacctgatg gtggagaaca agaaggtgaa gtttggcatg 780aatgtaacct cctctgagaa ggtggacaaa gcccagcgct atgccgactt cactctcctc 840tccatcccgt atccaggctg tgaatttttc aaggaatata aagatcggga ttacatggca 900gaagggctca tatttaactg gaagcaggac tacgttgatg ccccattgag catccccgac 960ttcctgactc actctctgaa cattgactgg agccagtatc agtgttggga tctggtgcaa 1020caaacacaaa actacctgaa gctgctgctt tccttagtta acagtgatga tgacagcggg 1080ctgctggtac actgtatctc aggctgggat cggacccccc tcttcatctc cctcctgcgc 1140ctttccttgt gggctgatgg gctcatccac acgtccctga agcccactga gatcctctac 1200ctcactgtgg cctatgactg gttcctcttc gggcacatgt tggtagatcg gctcagcaaa 1260ggggaggaga ttttcttctt ctgcttcaat tttttgaagc atattacctc cgaggagttc 1320tctgctctga agacccagag gaggaagagt ttgccagccc gggatggagg cttcaccctg 1380gaagacatct gcatgctgag acgaaaggac cgtggcagca ccaccagcct tggcagcgac 1440ttctccctgg tcatggagag ttccccagga gccactggga gcttcaccta tgaggccgtg 1500gagctggtcc cagcaggagc gccaactcag gcagcttggc ttgcagccct gagtgatcga 1560gagactcggc tgcaggaggt gcgctcagcc ttcttggctg cgtacagcag cacagtgggg 1620cttcgggcag tagcccccag tccttccggt gccatcgggg gcctgctgga gcaatttgcc 1680cgtggtgttg gactccggag catcagcagc aatgccttgt gaagaagcca gcccatgaca 1740ttttcctgct cctctctcag ctgagccctt agcagagaat caaagccatg cctggccgaa 1800ggggtacttc caggtcaggg gaaatttcag tcccccatct ccatcatgaa catggcagcc 1860ccaaagctga gcaaggccaa agacagggtt ttccaacccc cagcctcttg actggtgacc 1920accacccctt cttgtcactg tctcccaccc accccatctt tgctgggatt cccatcaact 1980ctcagaactg tgtggggttt ccctggggcc ttgtggaagc catgacttca caaagaccct 2040acctgtcagt tcttgtttct ggggaggagg gatcacctgc actgagaatg aggcagtttg 2100acacagatca caaaataaaa tcaaagtctt tttgaatagc caaaaaaaaa aaa 2153227791DNAHomo sapiensmisc_featureIncyte Clone No 2831245 227ttaactgagg actaagttga tctatgcagg gtctgagtcc aaaccctggt gtcaaggtgt 60taagtgcaaa ttattattat tattttttaa agaaaacact cttgttacaa tttggacaga 120gagaatggta tggagatgaa aggttctcgt gtatggcttt tgctcctatt tatgtggaaa 180gcacgcccta cattctttca aagctgtgtt gttcccttta ttctcagtcc ccagaattgt 240gtgcaaacac actctcttgg cccaggggtt tggctgggtg tgtttccttc tggaagtctt 300cactagcact cttgagttag ctggcaggag atcccttaaa accatttcca agcagttttt 360ctcacttccc tataggggct aatcctgtac tttccacttc agttccagct gctgttgctt 420gggaagaaac aaatttctgc tgtgttctca atctccagac ggtccatgaa aatttaatgt 480ataagaacaa agaggctggg cgcagtggct aacgcctgta atacctgcac tttgggaggc 540tgaggtgggt ggatcacctg aggtcagaag ttcgagaaca gcctagccaa catggcgaaa 600ccctgtctct actaaaaata ccaaatttgc tgaacgtgat ggtgggggct gttaacccca 660gtacttggga ggctgaggca ggaaatcgct gaactcggga agcaaaggtt gcattaaggg 720tacgagctcg aattcggtat catgttaaaa ccgtttccgg gttaaattgg tatccgccca 780caattcccac a 791228870DNAHomo sapiensmisc_featureIncyte Clone No 3116250 228cctgttctcg ccctcaaatg ggaacgctgg cctgggacta aagcatagac caccaggctg 60agtatcctga cctgagtcat ccccagggat caggagcctc cagcagggaa ccttccatta 120tattcttcaa gcaacttaca gctgcaccga cagttgcgat gaaagttcta atctcttccc 180tcctcctgtt gctgccacta atgctgatgt ccatggtctc tagcagcctg aatccagggg 240tcgccagagg ccacagggac cgaggccagg cttctaggag atggctccag gaaggcggcc 300aagaatgtga gtgcaaagat tggttcctga gagccccgag aagaaaattc atgacagtgt 360ctgggctgcc aaagaagcag tgcccctgtg atcatttcaa gggcaatgtg aagaaaacaa 420gacaccaaag gcaccacaga aagccaaaca agcattccag agcctgccag caatttctca 480aacaatgtca gctaagaagc tttgctctgc ctttgtagga gctctgagcg cccactcttc 540caattaaaca ttctcagcca agaagacagt gagcacacct accagacact cttcttctcc 600cacctcactc tcccactgta cccaccccta aatcattcca gtgctctcaa aaagcatgtt 660tttcaagatc attttgtttg ttgctctctc tagtgtcttc ttctctcgtc agtcttagcc 720tgtgccctcc ccttacccag gcttaggctt aattacctga aagattccag gaaactgtag 780cttcctagct agtgtcattt aaccttaaat gcaatcagga aagtagcaaa cagaagtcaa 840taaatatttt taaatgtcac aaaaaaaaaa 870229764DNAHomo sapiensmisc_featureIncyte Clone No 3129630 229gcacctgcga ccaccgtgag cagtcatggc gtactccaca gtgcagagag tcgctctggc 60ttctgggctt gtcctggctc tgtcgctgct gctgcccaag gccttcctgt cccgcgggaa 120gcggcaggag ccgccgccga cacctgaagg aaaattgggc cgatttccac ctatgatgca 180tcatcaccag gcaccctcag atggccagac tcctggggct cgtttccaga ggtctcacct 240tgccgaggca tttgcaaagg ccaaaggatc aggtggaggt gctggaggag gaggtagtgg 300aagaggtctg atggggcaga ttattccaat ctacggtttt gggatttttt tatatatact 360gtacattcta tttaaggtaa gtagaatcat cctaatcata ttacatcaat gaaaatctaa 420tatggcgata aaaatcattg tctacattaa aacttcttat agttcataaa attatttcaa 480atccatcatc tctttaaatc ctgcctcctc ttcatgaggt acttaggata gccatgattt 540cagtttcaca taagaatgtt tactcaatgt ttaagtgtgt tgccccaaaa ttcccaacta 600acaaggcaga actaggggac ttgaccttgg gacctttttg ggtcctaaac tccaggtaag 660tataaacaat ttcaattggc ctttcccctt gccaagaaaa aaaaaaataa aggggcgggg 720gggttccccg acccccggaa tttccggaaa cccttggtaa aacc 764230540DNAHomo sapiensmisc_featureIncyte Clone No 007632 230atcttgtggc gatcatgtat aagctggcct cctgctgttt gcttttcata ggattcttaa 60atcctctctt atctcttcct ctccttgact ccagggaaat atcctttcaa ctctcagcac 120ctcatgaaga cgcgcgctta actccggagg agctagaaag agcttccctt ctacagatac 180tgccagagat gctgggtgca gaaagagggg atattctcag gaaagcagac tcaagtacca 240acatttttaa cccaagagga aatttgagaa agtttcagga tttctctgga caagatccta 300acattttact gagtcatctt ttggccagaa tctggaaacc atacaagaaa cgtgagactc 360ctgattgctt ctggaaatac tgtgtctgaa gtgaaataag catctgttag tcagctcaga 420aacacccatc ttagaatatg aaaaataaca caatgcttga tttgaaaaca gtgtggagaa 480aaactaggca aactacaccc tgttcattgt tacctggaaa ataaatcctc tatgttttgc 540231857DNAHomo sapiensmisc_featureIncyte Clone No 1236968 231cacatttgaa cgcgcatgga cttccttcta cctaaacttt cgaacttttt ttagacacag 60gaagtagcaa gaagggagat gccaagtgac aatcaccagg aagatgcctc tctctagtga 120cctgggtagt ttgcacggtt tggctggaaa ccacagtccc cccatctctg ccagaacccc 180ccatgtggcc actgtcctca gacagctcct ggagcttgtg gataagcact ggaatggctc 240cggctccctc ctcctcaaca agaagtttct cggaaagttt gaagcaaaaa ctggtcagag 300tgctggagga aaacctcatt ttgtcagaaa aaattcaaca gttggaggaa ggtgctgcca 360tctcaattgt gagtgggcaa cagtcacata cttatgatga tcttctgcac aaaaaccaac 420agctgaccat gcaggtggct tgcctgaacc aggagcttgc ccagctgaaa aagctggaga 480agacagttgc cattctccat gaaagtcaga gatccctggt ggtaactaat gagtatctgc 540tgcagcagct gaataaggag ccaaaaggtt attccgggaa agcgctcctg cctcctgaga 600agggtcatca tctggggaga tcatcgccct ttgggaaaag cacgttgtct tcctcctcac 660cagtggcaca tgagactggt cagtatctaa tacagagcgt cttggatgct gccccagagc 720ctggcttata gagctagcat ggaactcaca ccacagcttc cctggtccac agaggctctc 780accgccattg ccaccagtat ggtggtatgt actcacaaag attaagaaag aaatgtattc 840tgattaaaaa aaaaaaa 8572321010DNAHomo sapiensmisc_featureIncyte Clone No 1334153 232gggaaccacc ttctgtagga cagtcaccag gccagatcca gaaggcttga ggccctgtgg 60tccccatcct tgggagaagt cagctccagc accatgaagg gcatcctcgt tgctggtatc 120actgcagtgc ttgttgcagc tgtagaatct ctgagctgcg tgccgtgtaa ttcatgggaa 180aaatcctgtg tcaacagcat tgcctctgaa tgtccctcac atgccaacac cagctgtatc 240agctcctcag ccagctcctc tctagagaca ccagtcagat tataccagaa tatgttctgc 300tcagcggaga actgcagtga ggagacacac attacagcct tcactgtcca cgtgtctgct 360gaagaacact ttcattttgt aagccagtgc tgccaaggaa aggaatgcag caacaccagc 420gatgccctgg accctcccct gaagaacgtg tccagcaacg cagagtgccc tgcttgttat 480gaatctaatg gaacttcctg tcgtgggaag ccctggaaat gctatgaaga agaacagtgt 540gtctttctag ttgcagaact taagaatgac attgagtcta agagtctcgt gctgaaaggc 600tgttccaacg tcagtaacgc cacctgtcag ttcctgtctg gtgaaaacaa gactcttgga 660ggagtcatct ttcgaaagtt tgagtgtgca aatgtaaaca gcttaacccc cacgtctgca 720ccaaccactt cccacaacgt gggctccaaa gcttccctct acctcttggc ccttgccagc 780ctccttcttc ggggactgct gccctgaggt cctggggctg cactttgccc agcaccccat 840ttctgcttct ctgaggtcca gagcaccccc tgcggtgctg acaccctctt tccctgctct 900gccccgttta actgcccagt aagtgggagt cacaggtctc caggcaatgc cgacagctgc 960cttgttcttc attattaaag cactggttca ttcactgccc aaaaaaaaaa 10102331981DNAHomo sapiensmisc_featureIncyte Clone No 1396975 233cagcactttg ggaggctggt ctcgaactcc tgatctcagg tgattcaccc gcctcagcct 60tccaaagtgc tgggattata ggtgtgagcc accgcgcccg gcctggatct gttttcttag 120cacgcagtga ggaatctttg tacttaaggc cagggcaaca aagtcaagag gtcaaggtgt 180agggccatga ggcctggacc tatgctgcag gcaagggttt ccatccccgc tgccctaggc 240actctcttcc caaggccagg ttgggcacct ggggaggtca gttcagaaat atctagcaga 300gacctcttaa acccccatcc cagcacccca tcctgttgtt cccagagctg gtctcccatg 360agtgtgctag agccagatag ccgtggcccc ccacccatct cactcacaca cacaggcatc 420catacacccc agaagacttc ccaaatgagg ccagactcag ggtcacgggg aatgtgcttc 480tgcccctgta agggctttgg ggaagggggc aacatagtag aggctggaaa gagcccccaa 540acctgtgccc atgcccctcc agccctgcgt ttccattctg ccttctcaga gtgcccttgc 600tgcacccaga ccaccggcca ggagagacct tctctcccac tccagcccct ctcactgccc 660ttcaactaga gctttcacct ttttacattt cccttctgaa ggacacaaat ctgcttttct 720gcccatacac tggcccaagg gctcacctaa cttgggaggg aaggggctgt tggtacaagg 780atgattttct gttagactgc cattttgcac ggtctccccc ttcccatctg atgtgtcctg 840cccctcagct ctttgcctta tctgtgtcac tgtcacttta gcaaaaatac agcggccatt 900tgtatcagcc tctggtggtt gcttgtgagg tgggactctt gcgggaacag gtggactttg 960ggaggagtgg gcagggaggg agtggtagtg gcagttctcg agctatctga ttaagccatt 1020ccgttagttc agttgtgccc tggagggcag gggacagggt cagtatctct ggggctgcag 1080gccctcttgc cttggccctc ctggcatggg gtaaccacca gctcagctct cctcctccag 1140ctttcctctc tctagcacac cccagccagg gcaaggatgc ccacgggcat agctacagca 1200acccctgcgg gatttggtgt ccacacccga gaggccaggc cagatgggaa agggattagc 1260gcctcttccc tcacactctg ccaggctgcc gggagcttgg gccaggtcta aggtaatgag 1320gtgctcctct tcctgctgga aaaaccggac agactcagaa ccacaaaggc aggtgctgcc 1380agcctggcgc cttcctctct gcttaggctg ggtgagcttg tccaggcctg tgcctcaccc 1440cttctctctt ctaggctcag tgtatgctta atcaggcatg gtgcatcaga gcgggaagga 1500gccatcaaca gtgtatactt ctggagcctt ctactgataa acagaggccc cagaagacga 1560tttgacttac ctgagctccc agctgggact taaacccagg tgtgtctgag tcacaactct 1620tcggggatgc cgtggtgagc tggggctgag ctcctgtatt cccactcccc caccccaccc 1680ccactcctgc catatcaggg ctggtctctg tggactcagc ccagggctgc ctcctctttg 1740tcaccccaaa gtggggcagc cagggacagc cagggtgtgt tcagaatggg ttcttcctgc 1800agggcaggaa gggcagattg ttaaaggggc tgcggcccag accaccctgg tccctcctcc 1860ggcagtgact cagacccaca ctgtgccgtg cagctgtgtg ccctgcacac ccgcttgacg 1920gcgcactgct cacttctggg gggccctttc agaggcactt ttaaagcaaa taaaacattt 1980a 1981234744DNAHomo sapiensmisc_featureIncyte Clone No 1501749 234gcgcccggtt ctccctcgca gcacctcgaa gtgcgcccct cgccctcctg ctcgcgcccc 60gccgccatgg ctgcctcccc cgcgcggcct gctgtcctgg ccctgaccgg gctggcgctg 120ctcctgctcc tgtgctgggg cccaggtggc ataagtggaa ataaactcaa gctgatgctt 180caaaaacgag aagcacctgt tccaactaag actaaagtgg ccgttgatga gaataaagcc 240aaagaattcc ttggcagcct gaagcgccag aagcggcagc tgtgggaccg gactcggccc 300gaggtgcagc agtggtacca gcagtttctc tacatgggct ttgacgaagc gaaatttgaa 360gatgacatca cctattggct taacagagat cgaaatggac atgaatacta tggcgattac 420taccaacgtc actatgatga agactctgca attggtcccc ggagccccta cggctttagg 480catggagcca gcgtcaacta cgatgactac taaccatgac ttgccacacg ctgtacaaga 540agcaaatagc gattctcttc atgtatctcc taatgcctta cactacttgg tttctgattt 600gctctatttc agcagatctt ttctacctac tttgtgtgat caaaaaagaa gagttaaaac 660aacacatgta aatgcctttt gatatttcat gggaatgcct ctcatttaaa aatagaaata 720aagcattttg ttaaaaaaaa aaaa 744235979DNAHomo sapiensmisc_featureIncyte Clone No 1575240 235gggatgaagc ccagcaagtt cacagggatc cgggaagttg tgtggctgga aacccaggca 60gggctgcacc

acagggacca tttgctggag atgcagcact tgccacagcc accaccactg 120acagcatgac acccacaaaa agggagcctc cagctgcacc cctgctgctg cgagtacttc 180ctcagctgtc tgccatgagc ttaaggttaa gtaccaggag ggaggatatg attgggcaaa 240cctcaggcat gtgttcattc tgtagcttcc agaacatgcg aggagagagc atctggctcc 300tttgtctcga ggaggagggg gcaggactct gccagaactc actcgataaa agattttccc 360aaaaggaagg gtgttcagat gacaaaagtc cactacacca ctttccttgg ctatctgatg 420cacccccatc ttcccatgcg cgcacctcag aaatcaggct cccacctgac ataacacaac 480catgcctcac aaaaagacag tggtttatcc cttccctagg agaaaagaga ggcaatgcca 540agctgcttca tcaactgtta atacttcttc cagcccgcaa cccaggatat ctgcaggtgt 600ctctccctct ggtttggtca tggctctctc tgttctagaa tgtatgggtt aaagtcggct 660gccacaccat gccctcggca gtgtggtcca aggacccctg agggtcctca aggtccttcc 720tttcccaacc ccacgtggtt ttcttcagtc aggataccat actgcaacag accgaaggcg 780gaagcagcta tgaggatgca gcagccttct gttaagccag gctttaagga tctgcaaaaa 840tgtaaaacga tgccactcct actgatgaaa tatattgttt tggaaaatat aggtttaaaa 900atttttttaa ggtaacatgt aatggatgta tagtcttcaa atggatgaat aaatgttttt 960cagagttaaa aaaaaaaaa 979236760DNAHomo sapiensmisc_featureIncyte Clone No 1647884 236cccgactgtg cgccgcggct ggctcgggtt cccgggccga catgggcgcc gccgcgtggg 60cacggccgct gagcgtgtct ttcctgctgc tgcttctgcc gctcccgggg atgcccgcgg 120gctcctggga cccggccggt tacctgctct actgcccctg catgggtaag gcctcccaag 180ccctctgctc agatggagaa actgaggccg ggagaggaaa agccactcct cagatgcgcc 240cagagacacc ttcacaggtc caggagagaa cctcagagcg ggacggggca tgctcttctc 300ctctctgcct tagttgcaag ggcacagagg ggccaacgtg tccaactttc catttgacag 360atgagaaaac tgaggctggg agaggttacg tgacttgctt gaggtctaag ccagtccagg 420gtccagtaaa tggagttagt ggggcaggac ttgatgtcac tgacccacgc tggctcctgg 480tgatttttca ttgattcagc aaatatttat ggggcaccta ttctgtgccg ggccctgttc 540tctgtactgg gaataccgca gtgaataaga taaactccgt gtccttgtag agccttcatt 600ttagttgggg aagacaaaca attgagaata agtaggccag gcgcggtggc tcacttctgt 660aatcccacca ctttgagaga ccgaggcagg atcacttgaa gccaggagct cgagatcagc 720ctaggcaaca tagtgaaaat ccaatctcaa aaaaaaaaaa 7602371080DNAHomo sapiensmisc_featureIncyte Clone No 1661144 237ttttttgtat ttttagtaga gatgggtcta accatgttgc ctaggctggt ctcgaactcc 60tgagctcaag cgatcctctt gcctcaacct cccaaagtgc tgggattgca gctgtgagcc 120accgcacccg gccgcattct tctaaatcac agtacatctg gttcccagtg cccaggctct 180cagggcagag ggtccagtgt gatcactttg catggcctct ctcccctcct gagcttgtgc 240cagggcccca gggctgacct ggagaaggaa aatggcagag ggtgaagatg gggtgtctgg 300tttggggacc atcctggccc cccttgtcac tgttggcatc tcttctgcac agtggcattg 360ctgggaggtg cttactgtgc ctattcaagg ggctggcagc cgcagcctca ctgcagatca 420gggacttggc ttcccggttg accacaggtc caagaacctg cagggtccag cctccccccc 480atccccagtc ttccccaccc tggcccggcc ctccaggtgc agaaacatgc aggcccctct 540ccaggactgt gggaggagtg tgtccctcag actggcctgt gtcctggctc ctcttaccac 600ctcttccaga ggttgtcacc tgcagctgcc ccaggataaa ggcaaggcca gagaggactc 660ctgaactcct gtgtgcctgg ggtggcaggg gcaaacatag ccaactggtg gcctgagcgg 720ggccatggtg aggacaccct tggtggcttg tcccacatca agctgggagg tgacactgag 780gatgcattag tctgcagcgt atgataaaaa cggcatttca ggccaggcgt ggtggctcat 840gcctgtcacc ccagcacctt gggaggccga ggtgggcgga tcatatgagg tcaggacttt 900gagaccagcc tggccaacat ggtgaaaact catctgtact aaaaaaacaa aaattatgtg 960ggttggtggt gtgcgcctgt aatcccagct acttgggagg ctgaggcagg agaatcactt 1020gaacctggga ggcggaggct acaacgagcc gaaattgcac cactgcactc cagcgctgat 10802381129DNAHomo sapiensmisc_featureIncyte Clone No 1685409 238caacgtccga cagaacgagg ggacgtaacg gaggcaggtt ggagccgctg ccgtcgccat 60gacccgcggt aaccagcgtg agctcgcccg ccagaagaat atgaaaaagc agagcgactc 120ggttaaggga aagcgccgag atgacgggct ttctgctgcc gcccgcaagc agaggtagcc 180ccagggaggg gagggaaagg gacggtggag acctgggtta gaccaagggt tatagaagga 240aagagagcta cctcagggct tgaatgtgga ctagtcgtga ggagcagagt gcattgcttc 300ctctagggtt ttatttcctc cccaccctcc aaattgttag ctcacagcct tacaggaaag 360gacgggggcg ggcgcctgcc ctcagtctga tttctgagcg tccctgggtc tgaccttaag 420ggcaagggca gggagcttca catttcaaat acagttgtgg ttacggcagc ccagtacttt 480tggccctcct tgctgttcgg ttctcctccc ttctcccaac ctcctcactg gtgttgctgg 540gtgtggtcct caatacagaa tagagaccct tgggcctgtg tcaccagact tctgacccct 600tgggcaacag ccagatggag actggtcgcc ttttgagcct cagctctctt cctcttgttc 660tcctagggtg ggagtacagc agccaaacgc tgaacttagt cccatccact tccatcttat 720cctttgtgcc cttcatcccc ctgcatcttg tcctttttgc cctctggtac ctcccagtgc 780cccatcatct ctacccccag ggactcggag atcatgcagc agaagcagaa aaaggcaaac 840gagaagaagg aggaacccaa gtagctttgt ggcttcgtgt ccaaccctct tgcccttcgc 900ctgtgtgcct ggagccagtc ccaccacgct cgcgtttcct cctgtagtgc tcacaggtcc 960cagcaccgat ggcattccct ttgccctgag tctgcagcgg gtcccttttg tgcttccttc 1020ccctcaggta gcctctctcc ccctgggcca ctcccggggg tgagggggtt accccttccc 1080agtgtttttt attcctgtgg ggctcacccc aaagtattaa aagtagctt 11292392370DNAHomo sapiensmodified_base(122)a, c, g, t, unknown, or othermodified_base(124)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 1731419 239agaaacttgg cccaagtttg tgacggttct gggtatgaaa gaagtcagtg tttcccaagt 60gcctgccatg tgcgaggctc tgtgctgggg cgggtgcatg tgtgcgttgg gggcgtggac 120angnggggtg ggaaaggcct gtgacatttc ctctggtggt ttccacgaac ccaggcgtca 180cccctcggtg gagataaagt ggagccaccc agctccaccg tgtctcagcc tggggtcggc 240ctctgctgct tctggactca gtgaccctgg gctgtcaggg agcttctgag ccttggtttt 300cctgtcgagt aagatggagg taatcgtgtc ttatggggtt gttttgaggg ttaaatgagc 360tggtggctgt gtgggaaaga gctctgcctc ccgcagggag gaactgtgct gttcttatta 420ttgtgaactt agtgacaagt gtggcactat tacccatttc cttgtctgcc cccaaccctg 480gggtcttggg cagagaacag gagttcttgc cattttctcc cagctcccac cttgtgctgg 540cttgcgggtg ctgaggtcat atttgctggg tgaaagggtg caggccagat atgagccagg 600cctggcagag agggttttgg tcagcagtga tacctgcagt gttctctgca gttggtttgg 660gctggccctg ctcctgagaa ctcctgggtt gtcccttcag gcaaccaggg aaggctcctt 720ggagcagcag catctcccct taccactcgc cgacaccagc ttccgcctga cccagagaag 780gagtttgggg acagccacag cacgtccagg gcttccaagg cagctggcag agccaatgag 840gagaccccaa cacccatccg acggctgcag ctctccctga cgtgtgttac cgcagccctg 900gtcccagccg ctgtgcttct cagggcctgc ctgcccagcc cgggtggata tggtgcccag 960gcgggccccg gggacacaat gagggccatt ctcagagcca ggcagagcgt gtggggcagt 1020cctgtcagtc ctatgtgcaa cagctgggat attgtttagg gagtgctggc atcaggccgg 1080ggctctcctc ctctggccct gccctttggg atgagcaagc ccccaaaggc cttcctgggt 1140tcctctggtg cacgtgccct ggagttaccc ttctgaagga ggtagacttg tcctcctgtc 1200ctgggtgcct ggggtgcagg ggtgtgaatt gggctatgtc aagatatgct gggcagtact 1260gtgaggtggg ggcagagggg agaaggtgtc ccaggaggag ccttcctgga ggggatgata 1320gtccagcatg ttctgaagtg ggagtagggt gcggcaggag tagggtacca gagaatgagt 1380gagtcaggca gcagcctcca ctgcgccttg gacacaggtg gccgacagtg tccacctgga 1440ctggctttgc accccttctg aggtcacagt tgtgtccctt gaaaacttgg gcaggagcac 1500ctgactggcc cagcttgggt catgccctag gcccagcagt gcgggaggcc aggaaagtag 1560gcttggggag gctggcctct cctccagttt gaagcatggc aggggttccg ggggaggctg 1620ctggggggcc tgcgagcatg tccagagcag gaatgcttgg ggtggtgtgt gctttgctcg 1680tctgggctta tctggccgtg gggaagctgg ttgtgcggat gacgttcact gagctgtgca 1740cgcatcatcc atggagtctg cggtgtgagt ccttttgccg ctccagggtc acagcctgcc 1800tccctgctcc agccccctgg ctgaggccct tcctctgccc catgctcttc tcagacagga 1860atcctgtgga atgtcatctc tttggggagg ccgtctctga ccctgtatgc aaaggccttc 1920tcccacatta tttttggcac cccactttct tccccgtgaa agcaaattgt ttggtgtctt 1980tctgtcccac tacagtatag gcccggttca gacagaggcc ttgtccacta ggcctgcgct 2040atctctgcgg agcccagcca aagcaggggc caggcgaatc ttttgttaaa agaacaatgc 2100gcgctgggca cagtgctcac gcctgtaatc ccagcacttt gggagtccga agctggagga 2160tcacttgaac ccaagagttt gagaccaccc tgggcaacat aaggagaacc catctctaca 2220caaaattagc tgggcgtggt ggtgtatgcc tgtagtccta gctacttggg aggctaaggt 2280gggaggtggc tgaggtggga ggatcacttg agcctgggag gttgttgcag tgagagccat 2340gatcgcgcta ctgggcaata gagcagaacc 2370240981DNAHomo sapiensmisc_featureIncyte Clone No 2650265 240cggactgccc tgagggcggg aaagggtggt cactgggtca gcccgaagca cctgacatga 60gggcggggac cccgaaatgc acacgaagtc cggaactggt cttctgtgat tttcattcgc 120cctggtctct gttccctttc gtactcaaag ctcgtgcatc cagggagggg aaaccggaga 180tagggtcttc gggccccggg cagaccctct gtgccgctgc aaaccgttgc agcctgaggc 240tgtcaggtcc tcccccagac acctgcggac cctccctctc ctggcttccc gtctggtcat 300ggcgagattc tgggtctgcg tagccggtgc tggcttcttt cttgcatttt tggttttgca 360ttcgcgtttt tgtggctctc cagttttgag gaactttact tttgcagttt cctggagaac 420tgagaaaatt ctttaccggc tggatgtggg ttggcctaag cacccagaat attttaccgg 480aacaacattt tgtgttgcag ttgactccct caatggattg gtttacatag gtcaaagagg 540ggataacatc ccaaagatat tagtgttcac agaggatgga tatttcctac gagcctggaa 600ttatacagtt gacacacctc atggtatatt tgcagccagt actctatatg aacaatccgt 660ctggatcacg gatgtaggaa gtggtatgta tagtaatatc tattaaatta tcttactgga 720aatcacatct ttgcacatgt ccttgtttgt attgtttaaa atcagagttg ctgaatctaa 780ttgtaatttc tttaacgatt catgaaatca catgttttta acaaacttta ttttgtactt 840ctgtggaatt aagaaattta acaagggctg gacgccgtgc tcacgcctgt aatcccagca 900ctttgggagg ccgaggcggg cggatcacga ggtcaggaga tcgagacgat cctggccaac 960acggtgaaac ccccgtctcc a 9812411204DNAHomo sapiensmisc_featureIncyte Clone No 2677129 241aggagaggaa ggtaattaca ttaagcatta taatatagtg tgttaaatgc taatgatcat 60aatcattgaa cccttctcag tcctcatctt atttaaatct ggtattttag cagacttttt 120tgccttactg ctattaatta attttttttt ggtctctttc ttccttgctt accctttgtt 180taacaaccaa atcaactcta gatcaatgaa tgaaataaaa aatctccagt acctacctcg 240gaccagtgaa ccccgcgaag ttctctttga agataggact agagctcatg ctgatcatgt 300cggtcagggg tttgactggc agagtacggc tgctgttgga gttttgaaag ctgtacaatt 360tggtgaatgg agtgaccaac ctcgcataac caaagatgtg atttgttttc atgctgagga 420ttttactgat gttgtacaaa gacttcagtt agatcttcat gaacctccag tttcccagtg 480cgtacagtgg gtagatgaag ctaaactaaa ccaaatgagg cgggaaggca ttcgttatgc 540tagaattcag ctttgcgaca atgatatcta cttcatccct agaaatgtca ttcatcagtt 600caaaacagtt tcggcggtgt gcagcttagc ctggcatata aggcttaaac agtaccaccc 660tgttgtggaa gccactcaaa acacagaaag caattctaac atggactgtg gtttaactgg 720aaagcgagaa ttagaagttg actcccaatg tgtgaggata aaaactgaat ctgaagaagc 780atgcacagag attcagctgt taacaactgc ttcatcatct ttcccacctg catcagaact 840taatctacag caagatcaga agactcagcc tattccagtt ttaaaagtgg aaagtagact 900ggactctgac cagcaacaca atctgcaaga acattcaacc acttctgtgt gatatgtaca 960tattcaaaca cattttttaa cttttttaaa ttttgatgtg aagttatagt tttataactg 1020gcttaagtta agttttattg gagaaatctt gcctataatt ctataaagag aaatgacatt 1080cacaaatgtc agcatatctt tttacacaga tatgcaagtt agagtgtatc tatccggtag 1140tacgtatgta taagtggtct gcgcacttct gttttaaggg tgaggtacat ccatctctct 1200cgag 1204242784DNAHomo sapiensmisc_featureIncyte Clone No 3151073 242cacagacaaa ccgtcaacag ctggtctcgc atgtcctttg ttcccggtct gctcttgtgt 60ttcgttctcc tcctgtgtgt tagccctgtg taccttccct ctcgttcacc ctccacattt 120cccatctctg agcccctcag ctttataggg atgtcagctt ggccccaatg tagtcccatt 180tacagccaga ctcctggact tgcctatgag ccatcttcat ttccaaaaag gcgatattgg 240gtatgtacat tgcatgaaat aaagtgggaa tgtcccagaa gcagaaggac atctgatgca 300gtccacgcca ataaattggg cttaccttta aaaatcatct gaatatgcag gtcttagggc 360agagaatata gacagcttaa gattttctaa actacaagtc ccacccaaaa tacggtattt 420tcatgatttc ccaaaggttg accatcagca agactggata tttttcagac ttaagatgac 480tgttcagtag ctgatgttct ggaaaagatc tgggccttca ccatgaaatc ttaaatgtga 540gcagttactg gatgttgaat ttgaaaccta ttcatttctt tttttaaaac aagcttggtc 600atttctgtgc aatgctataa ttcggaacga aacaaagcac aatgttaata aggtagacac 660taattcattc ctctgaagag agatctcttc cagacatttt aagccagggc aagaaatgtt 720taaagatgtt ttctgcagtt gccgtagaaa cactccttag cagtcatctt ggctgttggt 780aaaa 784243426DNAHomo sapiensmisc_featureIncyte Clone No 3170095 243ctccattaaa ccaccaccag ctccccaagc caccccttca gccatgaagt tcctgctcct 60ggtcttggca gccctcggat tcctgaccca ggtgatccca gccagtgcag gtgggtcaaa 120atgtgtgagt aacaccccag gatactgcag gacatgttgc cactgggggg agacagcatt 180gttcatgtgc aacgcttcca gaaaatgctg catcagctac tccttcctgc cgaagcctga 240cctaccacag ctcatcggta accactggca atcaaggaga agaaacacac aaaggaaaga 300caagaagcaa caaacgaccg taacatcata ataaccactg ctatcgcctc caccaactca 360gagaaatatc atttccacag ttccaattcc tcctacattg ctgagtacta gccaaggctc 420ctcttt 4262441732DNAHomo sapiensmodified_base(1651)a, c, g, t, unknown, or othermodified_base(1655)a, c, g, t, unknown, or othermisc_featureIncyte Clone No 3475168 244cgggaccaga gcacgttcct ggctgcagag gccacaagtc acgctgtctc tgagagtgga 60atgtcaccat cgcccaggtg gggatttttg tgtgttttgt tcactgctgt acacccagcc 120cccagcacag cgcctgtcca ggacaagtgc ccagtaaaca cttgggaagc aatgcaagcg 180tcctcccagc agctcctgca aacagacccc cgacccaagc ctttccttct gcctccactg 240ccaccactgc tgctcatctc tgctggcaca gaagtctctt ccctggtctt ccagaaatcc 300cctctccaca ctcagccaga gggagctatt aaaactgcgg gccagcccac atcagtccac 360agcaaagtcc tctctaaggg atctctgttg cttggagaat aaaccctcgg attccttcct 420tggctctcgg ggcctcctct ctgacctccc tctgtctcct ctcccagcct tcctcctcac 480tcaccctcca gccatgctgg cttcctcctt gctcctgaaa cagcctgaga gccacactgc 540cccgggccct ttgcactggc tgtttcctct gcctggagca cttctcctag gcatccacag 600ggctccctcc cacaactcct tcgggtgccc acatgggaag ccatccctga ccaccccccc 660gacttccttc tgagcaaggt agggtctttc tacctagtca tgagggcagg gatttttgtc 720tgttgtgttc tctgtgtgcc cccagtgcca tcccagtgcc tggcagatgg taagtgctcg 780acacacattg gctgactgcc tgaatgaaca actctatgag ccgatggcag ataaggacac 840tgaggtcctc tggggtaggt gaccagccca aggccacaca gctggtctga gattaggcca 900ggagaggagc ccgggttggt cacatcctgg agttggcgtc ttggaaactg catcaggaga 960ataacaaaga tgagacgcag gctctaacaa gtggatacca gtgactctcg ccccgccagc 1020cccagccctg cagccttggg cccttccagg agtcatggtc tgcctgcctg gggcattcca 1080ggcttcgacc caggtcctgc actttctatt ttgagcctct tagtcctgag gactgtgtgt 1140tcccagcagg cggcgcgggc cagaggctga gcctgggtgt ggctgtcacc ctatctgggg 1200ccagagaccc agattcccgg gcccttaacc tgttggctgc tgagggctct ggcataagcc 1260ctgttccctg cttgattgtc tccccttcaa gcccctgccc tggtatcgta tcggcccatc 1320tcaccttgga ttatatccct gtttggcccc atttgaatcc tggctctgcc cctttccagc 1380aatgtgacct tgggcaagtc acttcatctc tctggtctca gttcttcatc tggaaatggg 1440acaataagag tacctgtctc tggccatgtg tggtgactca tgcctgtaac cccagcgctt 1500tgggaagccg agccgagaga attgcttgag accaggagtt tgagatcagc cctgggcaac 1560atagtgagac ccctgtctct acaaaattct aaaaaaatta gccggttgtg gtggtgtgtg 1620cctgtagtcc cagctattct agaggctgag ncggnaggat tgcttgagcc cagcagtttg 1680aggctgcagt gagctatgat tatgcccgtg aaggcccccc aaaaaaaaaa aa 1732245918DNAHomo sapiensmisc_featureIncyte Clone No 3836893 245agcctctagg tcattgtggt gccttgtagc tgtcccggga gccctcagca gcagttggag 60ctggtgcaca ggaaggatga ggaagaccag gctctggggg ctgctgtgga tgctctttgt 120ctcagaactc cgagctgcaa ctaaattaac tgaggaaaag tatgaactga aagaggggca 180gaccctggat gtgaaatgtg actacacgct agagaagttt gccagcagcc agaaagcttg 240gcagataata agggacggag agatgcccaa gaccctggca tgcacagaga ggccttcaaa 300gaattcccat ccagtccaag tggggaggat catactagaa gactaccatg atcatggttt 360actgcgcgtc cgaatggtca accttcaagt ggaagattct ggactgtatc agtgtgtgat 420ctaccagcct cccaaggagc ctcacatgct gttcgatcgc atccgcttgg tggtgaccaa 480gggtttttca gggacccctg gctccaatga gaattctacc cagaatgtgt ataagattcc 540tcctaccacc actaaggcct tgtgcccact ctataccagc cccagaactg tgacccaagc 600tccacccaag tcaactgccg atgtctccac tcctgactct gaaatcaacc ttacaaatgt 660gacagatatc atcagggttc cggtgttcaa cattgtcatt ctcctggctg gtggattcct 720gagtaagagc ctggtcttct ctgtcctgtt tgctgtcacg ctgaggtcat ttgtacccta 780ggcccacgaa cccacgagaa tgtcctctga cttccagcca catccatctg gcagttgtgc 840caagggagga gggaggaggt aaaaggcagg gagttaataa catgaattaa atctgtaatc 900accagctaaa aaaaaaaa 918246676DNAHomo sapiensmisc_featureIncyte Clone No 4072159 246gctcacacag ctcccggcca ggtcacccgc catggtcctc cctctgccct ggctctctcg 60gtaccatttc cttcgcctcc ttctgccctc ctggtccttg gcaccccagg gctcccatgg 120gtgctgctcc caaaacccca aagcaagcat ggaagagcag accaactcca gaggaaatgg 180gaagatgacg tcccctccca ggggccctgg gacccaccgc acagctgagc tggcccgagc 240tgaagagttg ttggagcagc agctggagct gtaccaggcc ctccttgaag ggcaggaggg 300agcctgggag gcccaagccc tggtgctcaa gatccagaag ctgaaggaac agatgaggag 360gcaccaagag agccttggag gaggcgccta agtttccccc agtgcccaca gcaccctccg 420gcactgaaaa tacacgcacc acccaccagg agccttggga tcataaacac cccagcgtct 480tcccaggcca gagaaagtgg aagagaccac aaaccgcagg caattggcag gcagtggggg 540agccagggct ctgcagtctt agtcccattc ccctttgatc tcacagcagg cagggcacca 600caggccttac taggaattca ccctggacca tgccctaaaa taacctcacc ccaaatacaa 660taaagggacg aggcaa 6762472255DNAHomo sapiensmisc_featureIncyte Clone No 1003916 247ccggtgcgtc ctgggtctgt ctgcgcggag ttccccgggg cgcgaggaga ggggactgga 60gaaagaggag ggccgggcag cggaggggag gaggcggtgc gtgcctcgcc tgccaaaggg 120agatccgctc ctctgcgtgc gatccccggc gcccgcccgc gcccacagcg ctccgccaga 180gctgccgccg cggactcgcc gggagtgggg gtctccgctg gtgccagccc gcttctggag 240accctccgcc tcctgccaac ccctgctctt ccaggtcggg ccccggggtt ctgcggctgt 300tagggacaga ggcaaagaag ggcaggacgg tccggtttcc cgtggatgtt cccgcccgag 360aaagacagca agttgtgtgt gcgcccggga cgcgggaggg aaggtagccg ccgcccgcca 420gccatggacc atcatcttta gtgcagagga tggaaagttg atgcccagta agactgaaga 480tccattctgc attacggaac tgtggattat ctgtgggtcc ctggtgattt cacaccttca 540ttcactcctg cagtccctga acacttactt ggggtcctca ttgccctatc tggtgaaaga 600tggcatccag cctgacttgt actggagtaa tctgggcttt gctgtctttt ctttgtgctg 660ccacctcctg cgtggggttc tttatgcctt actggctctg gggatcacag ctgggcaagc 720ctgtgtcctt cggtaccttc cggaggtgct catatcctgt gcatgatgag agtcggcaga 780tgatggtgat ggtggaggaa tgtgggcgct atgcctcctt ccagggcatc cccagcgcag

840aatggaggat ctgcaccata gtgaccggcc tgggttgtgg cctcctcctc ctggtggcgc 900tcactgccct catgggttgc tgtgtttccg acctcatctc caggacagtg ggaagagtgg 960ctggaggaat tcagtttctt gggggcttgt tgattggtgc tggctgtgcc ctctacccct 1020tgggctggga cagtgaggaa gtccggcaga cttgtggcta cacttctggc cagtttgacc 1080tggggaagtg tgaaatcggc tgggcctact actgcacggg agcaggtgcc actgccgcca 1140tgctgctgtg cacgtggctg gcttgctttt cgggcaagaa acagaagcac tacccatact 1200gagatggagc taccaagagc agacagagga gaagatgggc caaaggggct tggagaggtc 1260aaaacatcca cctaccttca aaaggtggga tagtagttct aatccaatac aatgctaata 1320aaatgaaacc cgataaaatc aggaacatga tataggaagg aaggattgta ggagatttgt 1380gggggaaaaa aaaggagagt atagaatgat ggagaaaaat ggaccaaagg ctaaaaatat 1440tgcagggcat cgggtgtttc tattccacag agtattgtta atgtacaaca cacacacaca 1500cacacacaca cacacacaca cacacacaca acaaatctac atatacaaac aagggtttgg 1560gttttagttt ttttttttta aggtgaggac tcagaaaatc aaagggctag tagaaacagt 1620gttatgttgg gaagcagggt acccccaaag atgttccctg taggtcacgg cactcccaaa 1680agcacacaag cacatacaga catatgcatc cccacacacg cctatgcaca aacgtggatt 1740atcgcacaga ctgggaggtt tagtggtgca tttctcctct gttttctttt taatatacat 1800ttaaaataca gtattatcac tttataaaac atacattaag cctaataaat ggaccaataa 1860gccaaactat cagtattttg tatatcctgc ataaactcta atttagttcc tcaacatatt 1920ttcagtgttt atgcagacct ttagagttaa gcctttgtat ttccatgtta ttccacaata 1980tgcaatattt ctctgagtag cttctgctat gatattctta tgaagaaaag gggcaacttt 2040ctgtccacta taggagagaa ttcagccgaa gatatgagag taatgagaga cattttccag 2100tcattggatc gtgttttctt ttgtccatta ttgtactgtg ctgtaccaca tttatttcta 2160tattcatttt gtaaaaaatt taaaagtgct attttgtttg tatttgaaaa tctctgtgaa 2220taaattctct ctttgatcaa tagcaaaaaa aaaaa 22552481223DNAHomo sapiensmisc_featureIncyte Clone No 2093492 248gacgcttcac cagcgtcctg gtggtgtcca gtctctcacc cctgtgcgtg ctgctctgga 60gggaactcac aggcatccag ccaggcacat ccctgctcac cctgatgggc ttcaggctgg 120agggcatttt cccagcggcg ctgctgcccc tgttgctgac catgattctt ttcctgggcc 180cactgatgca gctctctatg gattgccctt gtgacctggc agatgggctg aaggttgtcc 240tggccccccg ctcctgggcc cgctgcctca cagacatgcg ttggctgcgg aaccaagtga 300tcgccccgct gacagaggag ctggtgttcc gggcctgtat gctgcccatg ttagcaccgt 360gcatgggcct gggccctgct gtgttcacct gcccgctctt ttttggagtt gcccattttc 420accatattat tgagcagctg cgtttccgcc agagcagcgt ggggaacatc ttcttgtctg 480ctgcgttcca gttctcctac acagctgtct tcggtgccta cactgctttc ctcttcatcc 540gcacaggaca cctgattggg ccggttctct gccattcctt ctgcaattac atgggtttcc 600cagctgtttg cgcggccttg gagcacccac agaggcggcc cctgctggca ggctatgccc 660tgggtgtggg actcttcctg cttctgctcc agcccctcac ggaccccaag ctctacggca 720gccttcccct ttgtgtgctt ttggagcggg caggggactc agaggctccc ctgtgctcct 780gacctatgct cctggatacg ctatgaactc tcaccggctc cccagccctc cccaccaagg 840ggtactgcag gggaagggct ggctggggtc cccgagatct caggaatttt tgtaggggat 900tgaagccaga gctagttgcg tcccagggac caagagaaag aagcagatat ccaaagggtg 960cagccccttt tgaaaggggt gtttacgagc agctgtgagt gaggggacaa ggggcaggtc 1020ccaggagcca cacactccct tcctcacttt ggactgctgc ttctcttagc tcctctgcct 1080ctgaaaagct gctcggggtt ttttatttat aaaacctctc cccacccccc accccccaac 1140ttcctgggtt ttctcattgt ctttttgcat cagtactttg tattgggata ttaaagagat 1200ttaacttggg taaaaaaaaa aaa 12232491188DNAHomo sapiensmisc_featureIncyte Clone No 2108789 249gcccctccca gcctgccaag aaaacggtag gggagcatga tggggccttt gaggcagggt 60cgcagggaca agctcagctt taggcaccat ctgttcccat cgcgcctgct gctgtgaccc 120gttttggaaa actggtgtgt accgaggcgc tgactgcacg gctgaccgcc tgctcgtgcc 180ttcattctgc agcggcatgg tccctcccat tctggctcca cctgcagcct ccctgggtgg 240cctaggctcc cccgaccaag agacctccct ctcatgatca ctggtacctg ggggcctgaa 300ttctggcccc cggctcccca cacagctggg actggcctgg atggctgtcc tggtagcccc 360tgcccaccct gacagaggga gctgggcctc ccctcatcct ctgtaactcc cgccttcacc 420agactcgagg acaccctggc cctgctgagg catacagagc ttcagcccag cacagaagca 480agacaaaatc agtggctctt agagtttaga aaacaagaca gactctcaga tgaaagatct 540gacaagcacc gtggccagtc acagggagag acttgatgtc tggcctttta attcctcctc 600tgccagggtg ggtcctggga cctctaatgt gggcatgtcg tccaccccag gacgagccat 660cagggacaga ccccccaccc ccaaggctgc agccacacca tgtttcaggc ttggggctgg 720ggcaggcttg ggctcaatcc tgggcaccca ggggcagccc acccctaacc tggctcctac 780ccaccttgcc cttgaaggat gggcctgctg cacgtctccc tcctccaccc cataccacac 840tggggggtct gagccacccc cctcagcccc gttcggctca gaccgacccc cactccatcc 900ccagacctgc agcacaagtg cgcgggcctg tcctcccagg ggcctgggcg actccatatg 960caatcagtag cgagcagccg ggccccacag accctcatgc actctcttac gtgccattct 1020ccccagactt tttttgtact taatgtatga aagatccaaa ctaatattgc tgtaaaaagg 1080agagacaaat taatatagct tattctataa atatatctgt atataaaggt ttctgtatat 1140tgtatagagc tgtgtataaa ctggatgtag aagcacaaaa aaaaaaaa 11882501792DNAHomo sapiensmisc_featureIncyte Clone No 2171401 250cgccgctggg gccggcccgc acggcttcat ctgagggcgc acggcccgcg accgagcgtg 60cggactggcc tcccaagcgt ggggcgacaa gctgccggag ctgcaatggg ccgcggctgg 120ggattcttgt ttggcctcct gggcgccgtg tggctgctca gctcgggcca cggagaggag 180cagcccccgg agacagcggc acagaggtgc ttctgccagg ttagtggtta cttggatgat 240tgtacctgtg atgttgaaac cattgataga tttaataact acaggctttt cccaagacta 300caaaaacttc ttgaaagtga ctactttagg tattacaagg taaacctgaa gaggccgtgt 360cctttctgga atgacatcag ccagtgtgga agaagggact gtgctgtcaa accatgtcaa 420tctgatgaag ttcctgatgg aattaaatct gcgagctaca agtattctga agaagccaat 480aatctcattg aagaatgtga acaagctgaa cgacttggag cagtggatga atctctgagt 540gaggaaacac agaaggctgt tcttcagtgg accaagcatg atgattcttc agataacttc 600tgtgaagctg atgacattca gtcccctgaa gctgaatatg tagatttgct tcttaatcct 660gagcgctaca ctggttacaa gggaccagat gcttggaaaa tatggaatgt catctacgaa 720gaaaactgtt ttaagccaca gacaattaaa agacctttaa atcctttggc ttctggtcaa 780gggacaagtg aagagaacac tttttacagt tggctagaag gtctctgtgt agaaaaaaga 840gcattctaca gacttatatc tggcctacat gcaagcatta atgtgcattt gagtgcaaga 900tatcttttac aagagacctg gttagaaaag aaatggggac acaacattac agaatttcaa 960cagcgatttg atggaatttt gactgaagga gaaggtccaa gaaggcttaa gaacttgtat 1020tttctctact taatagaact aagggcttta tccaaagtgt taccattctt cgagcgccca 1080gattttcaac tctttactgg aaataaaatt caggatgagg aaaacaaaat gttacttctg 1140gaaatacttc atgaaatcaa gtcatttcct ttgcattttg atgagaattc attttttgct 1200ggggataaaa aagaagcaca caaactaaag gaggactttc gactgcattt tagaaatatt 1260tcaagaatta tggattgtgt tggttgtttt aaatgtcgtc tgtggggaaa gcttcagact 1320cagggtttgg gcactgctct gaagatctta ttttctgaga aattgatagc aaatatgcca 1380gaaagtggac ctagttatga attccatcta accagacaag aaatagtatc attattcaac 1440gcatttggaa gaatttctac aagtgtgaaa gaattagaaa acttcaggaa cttgttacag 1500aatattcatt aaagaaaaca agctgatatg tgcctgtttc tggacaatgg aggcgaaaga 1560gtggaatttc attcaaaggc ataatagcaa tgacagtctt aagccaaaca ttttatataa 1620agttgctttt gtaaaggaga attatattgt tttaagtaaa cacattttta aaaattgtgt 1680taagtctatg tataatacta ctgtgagtaa aagtaatact ttaataatgt ggtacaaatt 1740ttaaagttta atattgaata aaaggaggat tatcaaattc aaaaaaaaaa aa 17922512005DNAHomo sapiensmisc_featureIncyte Clone No 2212530 251gcgaggcggg aggaggtgag gctccggcgc acacccaaac cgcgctgcgc ccgctccttc 60cgggccccgg agatggcgcc tccaccggga tgagctagcc agcctgggca ataccagagg 120cggccctcgg cgcgcgcagg ggaccgagct ggtcgcccca accgggtttg atttctgatg 180actctggcct gagttccagg atggtttttt cttgggacca gacatgaaca aaagttgacc 240tcatgagcac ttcaacctct ccagctgcca tgctcctccg gaggctgcgg cgactctcct 300ggggcagcac tgctgtccag ctcttcatcc taacagtggt gacgtttggc ctgctggccc 360ccctggcctg tcaccgactt ctacactctt acttctatct gcgccattgg catctgaacc 420aaatgagcca agagttcctg cagcaaagct tgaaagaggg tgaggctgcc ctccactatt 480ttgaggagct tccctctgcc aatggctcag tgcccattgt ctggcaggcc accccccggc 540cctggctggt gatcaccatc atcactgtgg acaggcagcc tggcttccac tacgtcctgc 600aggttgtgtc ccagttccac cggcttcttc agcaatgtgg cccccagtgc gaggggcacc 660aactcttcct gtgcaacgtg gagcgtagtg tgagccattt tgatgccaag ttgctctcca 720agtatgtccc tgtggccaat cgctatgagg gcactgagga tgattatggt gatgaccctt 780cgaccaactc gtttgagaaa gagaagcagg actatgtcta ttgcctggag tcatccctgc 840agacctacaa cccagactac gtcctgatgg tagaagacga tgctgtacca gaagagcaga 900tcttcccagt cttggagcac cttctgcggg ctcgcttctc tgagccacat ctcagagatg 960ccctttatct caagctgtat caccccgaga ggctccagca ctacatcaat ccagagccca 1020tgcggatcct ggaatgggtt ggtgtaggca tgttgctggg gcccttacta acctggatat 1080acatgaggtt tgccagccgc ccagggttta gctggcctgt aatgctcttc ttctccctgt 1140atagcatggg tctggtggag ctggtgggtc ggcactattt cctggaactg cggcggctga 1200gtccttccct gtacagtgtg gttcctgcct ctcagtgttg caccccagcc atgctcttcc 1260cggcacctgc ggcccgccgg accctcacct acctgtccca agtgtactgc cacaagggct 1320ttggcaagga catggcactg tactcgctgt tgagggccaa gggagagagg gcctatgtag 1380tggagccgaa cctcgtgaaa cacatcgggc tcttctccag tctccggtac aactttcatc 1440ccagtctcct ctagggtgcc aagagatgcc tttcggaagt tggccacttc ttgaagattc 1500aaatatttat ctctttattt agacatggtt gcctgcaggt atttcactgt ttactgttgt 1560tagagatata ggcactgggg cagctgagga acctcaatat gttaagagcc ttggctttgg 1620tagcctcctg gcaggagcag cagtttgcca caggtccgga cctctccctc cacacagcca 1680cactgcctca tgcagtctga cccacccagt gagggtgcat ttgaacactg attatattct 1740ccatttgttt ttaagctctg ctttgtgtta gagcttgtga ctgccaaaaa ttttgtgcac 1800agtgatatga ctgttttagg atcttaaggg tagaattttg tgaaaggtga gatcctttgg 1860aattgagttc tttctcattg ggtatgaaaa tggatgtatg tttagaatat atgcccaacg 1920aggcaggacc atgtggatag attccatttg tttccttgac ctgatgtaat aaaaactgat 1980aaaagccgtg cagtgcccgg catct 2005252471DNAHomo sapiensmisc_featureIncyte Clone No 2253036 252tgggtatgtc tcatggagag gtgctttcac tgcttccctg ttcacctagt cttcaatctg 60gtccagagtt tcagccccat ctctggagtt gagtcctgcc ttctccctca atgtgacaaa 120tgttggccaa tggtatatcg cagttgtgat gcaagcagag gcttggtaaa tgcctgcata 180ctggggtttg tcctcttgga atgctcattt gtgggagccc tgaacaacta tgtaagaagt 240ctggctaccc tgctggagag aacacatggt gggaagagac taaaattatg tgaagagagt 300caggccagcc atcccagctt ctctgctgag ccccgccatc agccaacctg ccagctgaat 360gcaaccgtaa gagtgatcac cagcaagatc actagaaaaa ccacctaact gagcccaccc 420tggattgaac aatcataaac aaataaaatg gttattgttt taaaaaaaaa a 4712533775DNAHomo sapiensmisc_featureIncyte Clone No 2280161 253tccctgagag gtgtccactg atgtctcctc ctcatttcat ttagcagctc ttagtttgtg 60aagaatctgt agatgctcaa agtataattg ccccagggaa tttatgctaa tcacaacctc 120ttcttcagac accaagtcta ccttgaatgg gtttgctgtg atatggcact tcaggtctcc 180ttttccatct gccaatatca gactgcctgt gtccccagag catgaaatca gccttacagt 240gcttggcttg cttgtgagga catccggaac ttcaaatctg ggttttagag gagtctcttc 300gttaatttta agcctgaaaa tgtttccttc tataccataa atttcagcca ggagaggaac 360cttacttgct tcattgatga tttggaacct ggtgctgtct tcatctgttg tgactgaatc 420caataatgcc cgataggtgg acttcttgga aagccactgt ttctgacgcc tgtaaaatgc 480gatcttgtta cagtctctga aaatgttttt atctacagct tcatcttcaa cacttatttc 540ctctttcact gctgcttcca tggcttctct gtcactccga ccagctccca gctctcagga 600caagggccct gggcgatctt ttaaaaaagc cgattgggtg tctttctaaa attacaacca 660gtacttcatc gtcaagtttc tgggaaggga gtcccctcca gattctcatg gagtgacaaa 720tcttgactct tgctcctgga atttttcagg cccaaactag cgtttctaca atgatttatt 780tggcaaattt gtcttgatta tgggtggctg atgaggaacg tgcttttgtt aggaaccgaa 840actgggcggc ggtgagggcg tgtacgcaat gagtccggaa gagggtgaaa tgctttcggt 900aggcactcca cggctgtgaa gatggcggcg gctgcgtggc ttcaggtgtt gcctgtcatt 960cttctgcttc tgggagctca cccgtcacca ctgtcgtttt tcagtgcggg accggcaacc 1020gtagctgctg ccgaccggtc caaatggcac attccgatac cgtcggggaa aaattatttt 1080agttttggaa agatcctctt cagaaatacc actatcttcc tgaagtttga tggagaacct 1140tgtgacctgt ctttgaatat aacctggtat ctgaaaagcg ctgattgtta caatgaaatc 1200tataacttca aggcagaaga agtagagttg tatttggaaa aacttaagga aaaaagaggc 1260ttgtctggga aatatcaaac atcatcaaaa ttgttccaga actgcagtga actctttaaa 1320acacagacct tttctggaga ttttatgcat cgactgcctc ttttaggaga aaaacaggag 1380gctaaggaga atggaacaaa ccttaccttt attggagaca aaaccgcaat gcatgaacca 1440ttgcaaactt ggcaagatgc accatacatt tttattgtac atattggcat ttcatcctca 1500aaggaatcat caaaagaaaa ttcactgagt aatcttttta ccatgactgt tgaagtgaag 1560ggtccctatg aatacctcac acttgaagac tatcccttga tgattttttt catggtgatg 1620tgtattgtat atgtcctgtt tggtgttctg tggctggcat ggtctgcctg ctactggaga 1680gatctcctga gaattcagtt ttggattggt gctgtcatct tcctgggaat gcttgagaaa 1740gctgtcttct atgcggaatt tcagaatatc cgatacaaag gagaatctgt ccagggtgct 1800ttgatccttg cagagctgct ttcagcagtg aaacgctcac tggctcgaac cctggtcatc 1860atagtcagtc tgggatatgg catcgtcaag ccacgccttg gagtcactct tcataaggtt 1920gtagtagcag gagccctcta tcttttgttc tctggcatgg aaggggtcct cagagttact 1980gggtattttt cttatccctt gactctgata gtaaacctgg ccctctcagc agttgacgcc 2040tgtgttattt tatggatatt tattagcctg actcaaacaa tgaagctatt aaaacttcgg 2100aggaacattg taaaactctc tttgtatcgg catttcacca acacgcttat tttggcagtg 2160gcagcatcca ttgtgtttat catctggaca accatgaagt tcagaatagt gacatgtcag 2220tcggactggc gggagctgtg ggtagacgat gccatctggc gcttgctgtt ctccatgatc 2280ctctttgtca tcatggttct ctggcgacca tctgcaaaca accagaggtt tgccttttca 2340ccattgtctg aggaagagga ggaggatgaa caaaaggagc ctatgctgaa agaaagcttt 2400gaaggaatga aaatgagaag taccaaacaa gaacccaatg gaaatagtaa agttaacaaa 2460gcacaggaag atgatttgaa gtgggtagaa gagaatgttc cttcttctgt gacagatgta 2520gcacttccag cccttctgga ttcagatgag gaacgaatga tcacacactt tgaaaggtcc 2580aaaatggagt aaggaatggg aagatttgca gttaaagatg gctaccatca gggaagagat 2640cagcatctgt gtcagtcttc tgtacggctc catgggatta aaggaagcaa tgacatcctg 2700atctgttcct tgatctttgg gcattggagt tggcgagagg tgtcagaaca aagagaacat 2760cttactgaaa acaagttcat aagatgagaa aaatctacga gcttcttatt tacaacactg 2820ctgccccctt tcctcccaga ctctgacatg gatgttcatg caacttaagt gtgttgttcc 2880tgaactttct gtaatgtttc attttttaaa tctgacaaac taaaaagttt aacgtcttct 2940aaaagattgt catcaacacc ataatatgta atctccagga gcaactgcct gtaattttta 3000tttatttagg gagttacata ggtgatgggg gaaattgtta actacctttc attttcctgg 3060gaagtcaagg ttacatcttg cagaggttgt tttgagaaaa aagggccctt ctgagttaag 3120gagccatagt tctatcaatg atcaaaagaa aaaaaaaaaa aagagaaact gttacagtat 3180gattcagatc atttaaaaaa gcaaaatcaa gtgcaatttt gtttacaaat ggtgtatatt 3240aaagattttt ctatttcaga tgtactttaa agagaaatat tagcttaact cttttgacat 3300ctgctattgt gacacatccc attgctggca atgtggtgca cactccgaaa cttttaacta 3360ctgttttgta agcctccaag ggtggcattg cagggtcctt aggcaatgtt ttgtttgcct 3420ttatgcagag aggtgctcca agtgctgtga ttgagcaccg tgctagagga actgtaatgc 3480ttcagaagtt gtagcttata caaaggaaac aggtcctgct ggcttaattt aaacagttat 3540tgcatgaagt agcgtggagg ccctggactg ctgctcgttc tttaggatgg actgttctgg 3600tatctggtat tggtttagag actgttaata agggacatca caaggtgatg ggattcattt 3660gaagcactct atttctgttt taatggtttt atccaatttt gccttcccaa gatttttgtt 3720ctacataaaa agttcatgcc actttttaat ataaaaaaat ttaacaaaaa aaaaa 37752541856DNAHomo sapiensmisc_featureIncyte Clone No 2287485 254cggccgcacc cggccggagc ggagggcaga gcgcgcgccc agttgcccgg gcaccaaatc 60ggagcgcggc gtgcgggagg gcccagagca ggactggaaa tgtcctggcc gcgccgcctc 120ctgctcagat acctgttccc ggccctcctg cttcacgggc tgggagaggg ttctgccctc 180cttcatccag acagcaggtc tcatcctagg tccttagaga aaagtgcctg gagggctttt 240aaggagtcac agtgccatca catgctcaaa catctccaca atggtgcaag gatcacagtg 300cagatgccac ctacaatcga gggccactgg gtctccacag gctgtgaagt aaggtcaggc 360ccagagttca tcacaaggtc ctacagattc taccacaata acaccttcaa ggcctaccaa 420ttttattatg gcagcaaccg gtgcacaaat cccacttata ctctcatcat ccggggcaag 480atccgcctcc gccaggcctc ctggatcatc cgagggggca cggaagccga ctaccagctg 540cacaacgtcc aggtgatctg ccacacagag gcggtggccg agaagctggg ccagcaggtg 600aaccgcacat gcccgggctt cctcgcagac gggggtccct gggtgcagga cgtggcctat 660gacctctggc gagaggagaa cggctgtgag tgcaccaagg ccgtgaactt tgccatgcat 720gaacttcagc tcatccgggt ggagaagcag taccttcacc acaacctcga ccacctggtc 780gaggagctct tccttggtga cattcacact gatgccaccc agaggatgtt ctaccggccc 840tccagttacc agccccctct gcagaatgcc aagaaccacg accatgcctg catcgcctgt 900cggatcatct atcggtcaga cgagcaccac cctcccatcc tgcccccaaa ggcagacctg 960accatcggcc tgcacgggga gtgggtgagc cagcgctgtg aggtgcgccc cgaagtcctc 1020ttcctcaccc gccacttcat cttccatgac aacaacaaca cctgggaggg ccactactac 1080cactactcag acccggtgtg caagcacccc accttctcca tctacgcccg gggccgctac 1140agccgcggcg tcctctcgtc cagggtcatg ggaggcaccg agttcgtgtt caaagtgaat 1200cacatgaagg tcacccccat ggatgcggcc acagcctcac tgctcaacgt cttcaacggg 1260aatgagtgcg gggccgaggg ctcctggcag gtgggcatcc agcaggatgt gacccacacc 1320aatggctgcg tggccctggg catcaaacta cctcacacgg agtacgagat cttcaaaatg 1380gaacaggatg cccgggggcg ctatctgctg ttcaacggtc agaggcccag cgacgggtcc 1440agcccagaca ggccagagaa gagagccacg tcctaccaga tgcccttggt ccagtgtgcc 1500tcctcttcgc cgagggcaga ggacctcgca gaagacagtg gaagcagcct gtatggccgg 1560gcccctggga ggcacacctg gtccctgctg ctggctgcac ttgcctgcct tgtccctctg 1620ctgcattgga acatccgcag atagaagttt tagaaagttc tatttttcca aaccaggatt 1680ccttactatt gacagatttt ctttaccaaa agaaaagaca tttattcttt tgatgcactt 1740gaatgccaga gaactgtcct tctttttctc ctctccctcc ctcccagccc ctgagtcatg 1800aacagcaagg agtgtttgaa gtttctgctt tgaactccgt ccagcctgat ccctgg 18562551545DNAHomo sapiensmisc_featureIncyte Clone No 2380344 255ggctggactg gaactcctgg tcccaagtga tccacccgcc tcagcctccc aaggtgctgt 60gattataggt gtaagccacc gtgtctggcc tctgaacaac tttttcagca actaaaaaag 120ccacaggagt tgaactgcta ggattctgac tatgctgtgg tggctagtgc tcctactcct 180acctacatta aaatctgttt tttgttctct tgtaactagc ctttaccttc ctaacacaga 240ggatctgtca ctgtggctct ggcccaaacc tgaccttcac tctggaacga gaacagaggt 300ttctacccac accgtcccct cgaagccggg gacagcctca ccttgctggc ctctcgctgg 360agcagtgccc tcaccaactg tctcacgtct ggaggcactg actcgggcag tgcaggtagc 420tgagcctctt ggtagctgcg gctttcaagg tgggccttgc cctggccgta gaagggattg 480acaagcccga agatttcata ggcgatggct cccactgccc aggcatcagc cttgctgtag 540tcaatcactg ccctggggcc aggacgggcc gtggacacct gctcagaagc agtgggtgag 600acatcacgct gcccgcccat ctaacctttt catgtcctgc acatcacctg atccatgggc 660taatctgaac tctgtcccaa ggaacccaga gcttgagtga gctgtggctc agacccagaa

720ggggtctgct tagaccacct ggtttatgtg acaggacttg cattctcctg gaacatgagg 780gaacgccgga ggaaagcaaa gtggccaggg aaggaacttg tgccaaatta tgggtcagaa 840aagatggagg tgttgggtta tcacaaggca tcgagtctcc tgcattcagt ggacatgtgg 900gggaagggct gccgatggcg catgacacac tcgggactca cctctggggc catcagacag 960ccgtttccgc cccgatccac gtaccagctg ctgaagggca actgcaggcc gatgctctca 1020tcagccaggc agcagccaaa atctgcgatc accagccagg ggcagccgtc tgggaaggag 1080caagcaaagt gaccatttct cctcccctcc ttccctctga gaggccctcc tatgtcccta 1140ctaaagccac cagcaagaca tagctgacag gggctaatgg ctcagtgttg gcccaggagg 1200tcagcaaggc ctgagagctg atcagaaggg cctgctgtgc gaacacggaa atgcctccag 1260tctctgtgcg cgatgccctg ttgaaccaga tggtccacgc cttccagcag ctgcagcagc 1320atcatggcgg cgaggcgggg gctgggtgtg ttcacacaaa ggaagaagag tgacctccct 1380ggaagaagat ggaattctgc cagcggccag gcttcaaacc tgaactgcac cgctggctcc 1440tccctggctc tccagcctgc ccgcctactc tgcggctttt aagactttgc caatccccat 1500agggagccag gtcctcaaaa taaacctgcc tctatataga cacat 15452561671DNAHomo sapiensmisc_featureIncyte Clone No 2383171 256gaattcggac gctctctggg ccaatatggc agcgcccagc aacaagacag agctggcctg 60gagtccgcgg ctggccgcgt gagtaggtga ttgtctgaca agcagaggca tgagctgggt 120ccaggccacc ctactggccc gaggcctctg tagggcctgg ggaggcacct gcggggccgc 180cctcacagga acctccatct ctcaggtccc tcgccggctc cctcggggcc tccactgcag 240cgcagctgcc catagctctg aacagtccct ggttcccagc ccaccggaac cccggcagag 300gcccaccaag gctctggtgc cctttgagga cctgtttggg caggcgcctg gtggggaacg 360ggacaaggcg agcttcctgc agacggtgca gaaatttgcg gagcacagcg tgcgtaagcg 420gggccacatt gacttcatct acctggccct gcgcaagatg cgggagtatg gtgtcgagcg 480ggacctggct gtgtacaacc agctgctcaa catcttcccc aaggaggtct tccggcctcg 540caacatcatc cagcgcatct tcgtccacta ccctcggcag caggagtgtg ggattgctgt 600cctggagcag atggagaacc acggtgtgat gcccaacaag gagacggagt tcctgctgat 660tcagatcttt ggacgcaaaa gctaccccat gctcaagttg gtgcgcctga agctgtggtt 720ccctcgattc atgaacgtca accccttccc agtgccccgg gacctgcccc aggaccctgt 780ggagctggcc atgtttggcc tgcggcacat ggagcctgac cttagtgcca gggtcaccat 840ctaccaggtt cctttgccca aagactcaac aggtgcagca gatccccccc agccccacat 900cgtaggaatc cagagtcccg atcagcaggc cgccctggcc cgccacaatc cagcccggcc 960tgtctttgtt gagggcccct tctccctgtg gctccgcaac aagtgtgtgt attaccacat 1020cctcagagct gacttgctgc ccccggagga gagggaagtg gaagagacgc cggaggagtg 1080gaacctctac tacccgatgc agctggacct ggagtatgtg aggagtggct gggacaacta 1140cgagtttgac atcaatgaag tggaggaagg ccctgtcttc gccatgtgca tggcgggtgc 1200tcatgaccag gcgacgatgg ctaagtggat ccagggcctg caggagacca acccaaccct 1260ggcccagatc cccgtggtct tccgcctcgc cgggtccacc cgggagctcc agacatcctc 1320tgcagggctg gaggagccgc ccctgcccga ggaccaccag gaagaagacg acaacctgca 1380gcgacagcag cagggccaga gctagtctga gccggcgcga gggcacgggc tgtggcccga 1440ggaggcggtg gactgaaggc atgagatgcc ctttgagtgt acagcaaatc aatgttttcc 1500tgcttggggc tctcttccct catctctagc agtatggcat cccctcccca ggatctcggg 1560ctgccagcga tgggcaggcg agacccctcc agaatctgca ggcgcctctg gttctccgaa 1620ttcaaataaa aaggggcggg agcgctgttg gttgtgcgca aaaaaaaaaa a 1671257792DNAHomo sapiensmisc_featureIncyte Clone No 2396046 257aattttaggg agaatgtggg ggggtggggt gttactttcc attttacaca tatttgtatt 60ttcagatttt caacaataac agtattcaat acataatcag aaaaaagaga tgtggaggag 120gaggagagaa acttcccaag gagctccctt gggtgctgct ggctcctaat tagtgtaacc 180tgttaatcac atgttgctcg gtgttagagc ggtccctctg tgctctgcct ggcagggcgc 240tgttggcctg gtctccctcg ctatttctat ttgcaagcat gggctttctt cccagcagaa 300tctggttcct gggaagagta atgttccaaa ggcctctgat atgcctcgat gccctcctgt 360cttccagagc cccaacctca ctccctttcc ccaccataca aaacacacct cccaggggtc 420acatttgggg gtcccgcccc ctgctccaat gccatggtgt ccccaagcac agggctttgg 480cctgagttgt cagtctctgg atgcatttga ggggcagcta gggtgtggct ggggggtcca 540agcagctggg gagccgagac tcagaatcat tcacacactt ctatttggag cttttgtgga 600agtttccaga attccataat attcacctcc tgaatggtgg ctgcccctta tcagccaggg 660ctggggtttc cagtgccctc ggagagcttg ctttagagtc ttggagagac ggccatggtc 720tgcgtttgta tgtctgtcac atcttaccat catcacaaat tgaatataca acattaccta 780attgtgtgat ca 7922583045DNAHomo sapiensmisc_featureIncyte Clone No 2456587 258gtgagagggg ctgatggaag ctgataggca ggactggagt gttagcacca gtactggatg 60tgacagcagg cagaggagca cttagcagct tattcagtgt ccgattctga ttccggcaag 120gatccaagca tggaatgctg ccgtcgggca actcctggca cactgctcct ctttctggct 180ttcctgctcc tgagttccag gaccgcacgc tccgaggagg accgggacgg cctatgggat 240gcctggggcc catggagtga atgctcacgc acctgcgggg gaggggcctc ctactctctg 300aggcgctgcc tgagcagcaa gagctgtgaa ggaagaaata tccgatacag aacatgcagt 360aatgtggact gcccaccaga agcaggtgat ttccgagctc agcaatgctc agctcataat 420gatgtcaagc accatggcca gttttatgaa tggcttcctg tgtctaatga ccctgacaac 480ccatgttcac tcaagtgcca agccaaagga acaaccctgg ttgttgaact agcacctaag 540gtcttagatg gtacgcgttg ctatacagaa tctttggata tgtgcatcag tggtttatgc 600caaattgttg gctgcgatca ccagctggga agcaccgtca aggaagataa ctgtggggtc 660tgcaacggag atgggtccac ctgccggctg gtccgagggc agtataaatc ccagctctcc 720gcaaccaaat cggatgatac tgtggttgca attccctatg gaagtagaca tattcgcctt 780gtcttaaaag gtcctgatca cttatatctg gaaaccaaaa ccctccaggg gactaaaggt 840gaaaacagtc tcagctccac aggaactttc cttgtggaca attctagtgt ggacttccag 900aaatttccag acaaagagat actgagaatg gctggaccac tcacagcaga tttcattgtc 960aagattcgta actcgggctc cgctgacagt acagtccagt tcatcttcta tcaacccatc 1020atccaccgat ggagggagac ggatttcttt ccttgctcag caacctgtgg aggaggttat 1080cagctgacat cggctgagtg ctacgatctg aggagcaacc gtgtggttgc tgaccaatac 1140tgtcactatt acccagagaa catcaaaccc aaacccaagc ttcaggagtg caacttggat 1200ccttgtccag ccagtgacgg atacaagcag atcatgcctt atgacctcta ccatcccctt 1260cctcggtggg aggccacccc atggaccgcg tgctcctcct cgtgtggggg gggcatccag 1320agccgggcag tttcctgtgt ggaggaggac atccaggggc atgtcacttc agtggaagag 1380tggaaatgca tgtacacccc taagatgccc atcgcgcagc cctgcaacat ttttgactgc 1440cctaaatggc tggcacagga gtggtctccg tgcacagtga cgtgtggcca gggcctcaga 1500taccgtgtgg tcctctgcat cgaccatcga ggaatgcaca caggaggctg tagcccaaaa 1560acaaagcccc acataaaaga ggaatgcatc gtacccactc cctgctataa acccaaagag 1620aaacttccag tcgaggccaa gttgccatgg ttcaaacaag ctcaagagct agaagaagga 1680gctgctgtgt cagaggagcc ctcgttcatc ccagaggcct ggtcggcctg cacagtcacc 1740tgtggtgtgg ggacccaggt gcgaatagtc aggtgccagg tgctcctgtc tttctctcag 1800tccgtggctg acctgcctat tgacgagtgt gaagggccca agccagcatc ccagcgtgcc 1860tgttatgcag gcccatgcag cggggaaatt cctgagttca acccagacga gacagatggg 1920ctctttggtg gcctgcagga tttcgacgag ctgtatgact gggagtatga ggggttcacc 1980aagtgctccg agtcctgtgg aggaggtgtc caggaggctg tggtgagctg cttgaacaaa 2040cagactcggg agccttgctg aggagaacct gtgcgtgacc accgccggcc cccacagctc 2100ctgaagtcct gcaatttgga tccctgccca gcaagtcctg tcatctagga agaagcagta 2160tcgactcagc atggaacgcc tgcaacgttc tttgttaggc aaccaagagg cctggcttct 2220catcctgctg tcaccaacta gctctgtggc ctagggcgag gtgtctgccc tttatgtttc 2280cacatctgca aagtgaactg gttgtacctg atgatctgag atcccatgac ttgctcacat 2340gtcccatgat tctttatttt gtaggcagaa gcattaaaca gctactcctg ctgctgtgtg 2400ctaatcattc ctgtaatttc tgttctgctt atttgccatt atttgaaaaa catgcaaaag 2460ggtctttcta accacattcc tgtgttgtaa caacacccaa atgctgaggc agtgccgagg 2520agtcagtgcc tgggacttgc ttaaaactgc tgggactcgt ggtccctaaa cccttctttg 2580agcaccaaaa cgaataggac atgagatgtt acttctcatt ctcaaagtac taactatgtt 2640taagttacaa aaggttaggt tatcctgtga cccttttgtt gactcacaga caagaacagt 2700tgttgagctt aatgttgtcg catttgctcc agataaactc aattctctga tttcccacca 2760gccaactgtc aagccaacag gcaagacctc tcactgggca cagccaggag tttcttgggt 2820cgaccataca cattgaaaca tttgtagaag gttgctaatt gcaacaataa aggggaccaa 2880agtataatgg cctaatctca tccaagagtc aaaacagatt ttccccctaa aaatgataat 2940tgtatagagg tgcctttcct gtggaatatc tcactctgat gtcagagaaa aatctctcct 3000tcccttctcc tggtgttcaa tgtgagacag aaaataaaat gtgtg 30452592445DNAHomo sapiensmisc_featureIncyte Clone No 2484813 259gcatcttggc agggtccggg gacgtggact atttcgcaca ccacaccacg gggagggatt 60tttttctatt ttccctacga aaaacagatc tttttaagga tggtgctgct ccactggtgc 120ctgctgtggc tcctgtttcc actcagctca aggacccaga agttacccac ccgggatgag 180gaactttttc agatgcagat ccgggacaag gcattttttc atgattcgtc agtaattcca 240gatggagctg aaattagcag ttatctcttt agagatacac ctaaaaggta tttctttgtg 300gttgaagaag acaatactcc attatcagtc acagtgacgc cctgtgatgc gcctttggag 360tggaagctga gcctccagga gctgccagag gacaggagcg gggaaggctc aggtgatctg 420gaacctcttg agcagcagaa gcagcagatc attaatgagg aaggcactga gttattctcc 480tacaaaggca atgatgttga gtattttata tcgtctagtt ccccatccgg tttatatcag 540ttggatcttc tttcaacaga gaaagacaca catttcaaag tatatgccac cacaactcca 600gaatctgatc agccataccc tgagttaccc tatgacccaa gagtagatgt gacctcactg 660gggcgcacca cggtcacttt ggcctggaaa ccaagcccca ctgcctcttt gctgaaacaa 720cccattcagt actgtgtggt catcaacaaa gagcacaatt tcaaaagtct ctgtgcagtg 780gaagcaaaac tgagtgcaga tgatgctttt atgatggcac cgaaacctgg tctggacttc 840agcccctttg actttgccca ctttggattt ccttctgata attcaggtaa agaacgcagt 900ttccaggcaa agccttctcc aaaactgggg cgtcatgtct actccaggcc caaggttgat 960attcagaaaa tctgcatagg aaacaagaac atcttcaccg tctctgatct gaaacccgac 1020acgcagtact actttgacgt atttgtggtc aacatcaaca gcaacatgag caccgcttat 1080gtaggtacct ttgccaggac caaggaagaa gccaaacaga agacagtcga gctaaaagat 1140gggaagataa cagatgtatt tgttaaaagg aagggagcaa agtttctacg gtttgctcca 1200gtctcttctc accaaaaagt caccttcttt attcactctt gtctggatgc tgtccaaatc 1260caagtgagaa gagatgggaa acttcttctg tctcagaatg tggaaggcat tcagcagttt 1320cagcttagag gaaaacctaa agctaaatac ctcgttcgac tgaaaggaaa caagaaagga 1380gcatctatgt tgaaaattct agctaccaca aggcctacta agcagtcatt tccctctctt 1440cctgaagaca caagaatcaa agcctttgac aagctccgta cctgttcctc ggccaccgtg 1500gcttggctag gcactcagga aaggaacaag ttttgcatct acaaaaaaga agtggatgat 1560aactacaatg aagaccagaa gaaaagagag caaaaccaat gtctaggacc agatataagg 1620aagaagtcag aaaaggtcct ctgtaaatat ttccacagtc aaaacctgca gaaagcagtg 1680accacagaaa caattaaagg tcttcagcct ggcaaatctt acctgctgga tgtttatgtc 1740ataggacatg gggggcactc tgtaaagtat cagagtaagg ttgtgaaaac tagaaagttc 1800tgttagttac cttcttatag agatatatta tgtagaactc caggagggac attaaatcac 1860tttaagtata aactgactac tcccacagtt gagagaagtt gtgacctgta cttgtactat 1920ggaaggaagg atatcaacgt gtgtatattg atgtttatat aagtaactct tgaaggagac 1980ttgttctagc gtgccccatg gtacctagtg tgtgtctgat gccggttggt gtcaaagata 2040gagggcttct tgaaggaact tgccattcct tgctttgacc actgcatgaa ctgcttctaa 2100attattttat tacctaaaaa tttaaaatat gccattcatt gcacacaccc acaaatgcaa 2160atcattcctc tctatagatg ctaggatata tataaattat tttataaatt cttgttttaa 2220atgtcagtgt ttctatgatt gtaaactatt aaattctttt cctattaaag tacagatcta 2280atctaagtat tattaagttg atagccctct agtcagttat attgctattg taaattcttg 2340tttgttgagt aaaatgttta aatactatat gtatctcatg tacaaagttg acatacatta 2400tattcatgta cataaaatta aagagattag attatatagt gttca 2445260672DNAHomo sapiensmisc_featureIncyte Clone No 2493851 260cccacgggcg cccagcctag gagtcgtccc ccaggcaatc cccagtactc ctgatgctgg 60agagccagcc acactgcaca gtgccccggg ggcggtttct accaccctaa ggggtattct 120tggctccagg catcagagtc catgtggctt gtggggccct catttctttc atgcccactg 180gggaaggttc caccagcagg gctgttactg gcggggtcct ctgggagggg ggcaagaagg 240ccagccacac caaggcactg gagctccacg actcctggcc ttcgattgga ggcccctctc 300tgccagctct gccccttggg gggcaccagg caggactgcc agccgctctc ctggcaggtg 360acatcagcct tcaagctcac tgtgccctca ccatttcatg ctcccccaag gtcctggtca 420tgtcttctct tgggtatctt cccaggacag gcactggcac tggagccctg gcacttgttt 480ctgggttcca tgcttcccag gtgtgatggt gaatgctgag tgtcagcttg actggattga 540aggatgcaaa gtattgtcac tgggtgtgtc tgtgagggtg ttgccagagg agattcccat 600ttgagtcagt gggctgggag aggcagaccc accctcaatc caggtgggca ccacctaatc 660ggctgccagc aa 6722611183DNAHomo sapiensmisc_featureIncyte Clone No 2495719 261gagagaaatg atgtgacagg agcaagcgaa ctacaacccc gccccgccgt tcctgcccca 60ccactgcggc ggcgggcgct acgttccgga agcggaaatg gacgagaggt cagggtaggt 120ttttgaagat ggcggccctc aaggctctgg tgtccggctg tgggcggctt ctccgtgggc 180tactagcggg cccggcagcg accagctggt ctcggcttcc agctcgcggg ttcagggaag 240tggtggagac ccaagaaggg aagacaacta taattgaagg ccgtatcaca gcgactccca 300aggagagtcc aaatcctcct aacccctctg gccagtgccc catctgccgt tggaacctga 360agcacaagta taactatgac gatgttctgc tgcttagcca gttcatccgg cctcatggag 420gcatgctgcc ccgaaagatc acaggcctat gccaggaaga acaccgcaag atcgaggagt 480gtgtgaagat ggcccaccga gcaggtctat taccaaatca caggcctcgg cttcctgaag 540gagttgttcc gaagagcaaa ccccaactca accggtacct gacgcgctgg gctcctggct 600ccgtcaagcc catctacaaa aaaggccccc gctggaacag ggtgcgcatg cccgtggggt 660caccccttct gagggacaat gtctgctact caagaacacc ttggaagctg tatcactgac 720agagagcagt gcttccagag ttcctcctgc acctgtgctg gggagtagga ggcccactca 780caagcccttg gccacaacta tactcctgtc ccaccccacc acgatggcct ggtccctcca 840acatgcatgg acaggggaca gtgggactaa cttcagtacc cttggcctgc acagtagcaa 900tgctgggagc tagaggcagg cagggcagtt gggtcccttg ccagctgcta tggggcttag 960gccatgctca gtgctgggga caggagtttt gcccaacgca gtgtcataaa ctgggttcat 1020gggcttaccc attgggtgtg cgctcactgc ttgggaagtg cagggggtcc tgggcacatt 1080gccagctggg tgctgagcat tgagtcactg atctcttgtg atggggccaa tgagtcaatt 1140gaattcatgg gccaaacagg tcccatcctc ttcaaaaaaa aaa 11832621266DNAHomo sapiensmisc_featureIncyte Clone No 2614153 262gcctgaccac gcagttcttg ggtctgtgct gctggcctgg ggttgtggtt gaggccgggt 60ctccgctcct gtgcccggga agatggtgct aggtggttgc ccggttagtt acttacttct 120gtgcggccag gcggctttgc tgctggggaa tttacttctg ctgcattgtg tgtctcggag 180ccactcgcaa aatgcgaccg ctgagcctga gctcacatcc gctggcgccg cccagccgga 240gggccccggg ggtgctgcga gctgggaata tggcgacccc cactctccgg tcatcctctg 300ctcttaccta cctgatgaat ttatagaatg tgaagaccca gtggatcatg ttggaaatgc 360aactgcatcc caggaacttg gttatggttg tctcaagttc ggcggtcagg cctacagcga 420cgtggaacac acttcagtcc agtgccatgc cttagatgga attgagtgtg ccagtcctag 480gacctttcta cgagaaaata aaccttgtat aaagtatacc ggacactact tcataaccac 540tttactctac tccttcttcc tgggatgttt tggtgtggat cgattctgtt tgggacacac 600tggcactgca gtagggaagc tgttgacgct tggaggactt gggatttggt ggtttgttga 660ccttattttg ctaattactg gagggctgat gccaagtgat ggcagcaact ggtgcactgt 720ttactaaaaa gagctgccat catggcccag ggaggcgggt gaaagctccg tcttctgaat 780tcatctctac aggctcaaaa ctcctctttg atatcagacc tgatgttatt ttccttcttt 840tggagggcat ttgtttggtt aagaaggctt ctttggactt tggaatttca acccagattt 900taccttgcag acggaatgac aagcaaaaag tgttgtgggg aatcaaattt gttcctttcc 960tcatgcacaa aacataaagg atagtggcga gtttacaagc tgtggatggg tttccatagt 1020cttcctttct gtacattgct atatcttcag tcctttggag caagtggacc taacaagttg 1080agcaaaatga atatttggat ccatgttcct cttgtgaccc tgagtcttca tgcaaggaga 1140tctgaagctg aacaatgaaa atcttcagca gaaatagaaa tggccgtgga ttgtaataca 1200cactgaaatt ctgactttct gaatttaaat gtagaataaa ttttaccaac ttggaaaaaa 1260aaaaaa 12662631093DNAHomo sapiensmisc_featureIncyte Clone No 2655184 263gatggcttgt ttttcatttt ttttgtgctt tttggtccat ctattaataa aaatgaaccc 60cgttacagag tcaccatcat gtctcttctc accaccctct gaatctgcat tagccagtca 120actagccctt tcagcgtcat gtgaccagcg cgccccattc agcttggctg gtgtcgtttc 180acatgaccca ggctggccag tcgtcaggtt gcaccgccct ttggttcccg agcatgctgt 240tttctctcag ccttctctcc aaccttaacc aaatcggcag cagccacctc gaccgcccac 300acattcctgg ccaatcagct cagctgttta tttaccaaat gtcttcacaa caactacagc 360agcagccttc ggctaacaaa aaagcaggaa aaatccacaa cacccccttc gccaaccaac 420taaatccaac gcaacatctg gcaaaacctt ttcagcaaat tcttcctggc cgtcagtccg 480gcagcctcac ctcaccattt ctagcttgtt gaaacccaaa actaatctcc aagaaggaga 540agcttctctc gcagccggag caggtccctt tctagagata ggagaagaga gagatcgctg 600tctcgggaga gaaatcacaa gccgtcccga tccttctcta ggtctcgtag tcgatctagg 660tcaaatgaaa ggaaatagaa gacagtttgc aagagaagtg gtgtacagga aattacttca 720tttgacagga gtatgtacag aaaattcaag ttttgtttga gacttcataa gcttggtgca 780tttttaagat gttttagctg ttcaaatctg tttgtctctt gaaacagtga cacaaaggtg 840taattctcta tggtttgaaa tggatcatac gaggcatgta ataccaagaa ttgttacttt 900acaatgttcc cttaagcaaa attgaatttg ctttgaactt ttagttatgc acagactgat 960aataaacctc taaacctgcc cagcggaagt gtgttttttt taaatttaaa tacagaacca 1020ctggcaaaaa ttgaactaag atttactttt ttttccatag ctgggatata ggggggatcc 1080tctagagtcg acc 10932641056DNAHomo sapiensmisc_featureIncyte Clone No 2848362 264gcctgacatg cctgatcctc tcttttctgc agttcaaggg aaagacgaga tcttgcacaa 60ggcactctgc ttctgccctt ggctggggaa gggtggcatg gagcctctcc ggctgctcat 120cttactcttt gtcacagagc tgtccggagc ccacaacacc acagtgttcc agggcgtggc 180gggccagtcc ctgcaggtgt cttgccccta tgactccatg aagcactggg ggaggcgcaa 240ggcctggtgc cgccagctgg gagagaaggg cccatgccag cgtgtggtca gcacgcacaa 300cttgtggctg ctgtccttcc tgaggaggtg gaatgggagc acagccatca cagacgatac 360cctgggtggc actctcacca ttacgctgcg gaatctacaa ccccatgatg cgggtctcta 420ccagtgccag agcctccatg gcagtgaggc tgacaccctc aggaaggtcc tggtggaggt 480gctggcagac cccctggatc accgggatgc tggagatctc tggttccccg gggagtctga 540gagcttcgag gatgcccatg tggagcacag catctccagg agcctcttgg aaggagaaat 600ccccttccca cccacttcca tccttctcct cctggcctgc atctttctca tcaagattct 660agcagccagc gccctctggg ctgcagcctg gcatggacag aagccaggga cacatccacc 720cagtgaactg gactgtggcc atgacccagg gtatcagctc caaactctgc cagggctgag 780agacacgtga aggaagatga tgggaggaaa agcccaggag aagtcccacc agggaccagc 840ccagcctgca tacttgccac ttggccacca ggactccttg ttctgctctg gcaagagact 900actctgcctg aacactgctt ctcctggacc ctggaagcag ggactggttg agggagtggg 960gaggtggtaa gaacacctga caacttctga atattggaca ttttaaacac ttacaaataa 1020atccaagact gtcatattta gctggaaaaa aaaaaa 10562651183DNAHomo sapiensmisc_featureIncyte Clone No 2849906 265ggagctcagc cgagggctgc acaaagacct tcctggcctg ccccagacag agctgaggac 60ccctggccgt gggcttgggc ctcggcttca caggatgggg ctgccagtgt cctgggcccc

120tcctgccctc tgggttctag ggtgctgcgc cctgctcctc tcgctgtggg cgctgtgcac 180agcctgccgc aggcccgagg acgctgtagc ccccaggaag agggcgcgga ggcagcgggc 240gaggctgcag ggcagtgcga cggcggcgga agcgtcccta ctgaggcgga cccacctctg 300ctccctcagc aagtcggaca ccagactgca cgagctgcac cggggcccgc gcagcagcag 360ggccctgcgg cctgccagca tggatctcct gcgcccacac tggctggagg tgtccaggga 420catcaccgga ccgcaggcag ccccctctgc cttcccacac caggagctgc cccgggctct 480gccggcagct gcagccaccg cagggtgcgc tggcctcgag gccacctatt ccaacgtggg 540gctggcggcc cttcccgggg tcagcctggc ggccagccct gtggtggccg agtatgcccg 600cgtccagaag cgcaaaggga cccatcgcag tccccaagag ccacagcagg ggaagactga 660ggtgaccccg gccgctcagg tggacgtcct gtactccagg gtctgcaagc ctaaaaggag 720ggacccagga cccaccacag acccgctgga ccccaagggc cagggagcga ttctggccct 780ggcgggtgac ctggcctacc agaccctccc gctcagggcc ctggatgtgg acagcggccc 840cctggaaaac gtgtatgaga gcatccggga gctgggggac cctgctggca ggagcagcac 900gtgcggggct gggacgcccc ctgcttccag ctgccccagc ctagggaggg gctggagacc 960cctccctgcc tccctgccct gaacactcaa ggacctgtgc tccttcctcc agagtgaggc 1020ccgtcccccg ccccgccccg cctcacagct gacagcgcca gtcccaggtc cccgggccgc 1080cagcccgtga ggtccgtgag gtcctggccg ctctgacagc cgcggcctcc ccgggcatcc 1140tagagaaggc ccgcgtctaa ataaagcgcc acgcagagtg atc 1183266840DNAHomo sapiensmisc_featureIncyte Clone No 2899137 266gcatgtcatg gccgcctcca tggcccgggg aggcgtgagt gccagggttc tactgcaggc 60tgccaggggc acctggtgga acagacctgg gggcacttcc gggtcggggg agggggtggc 120gctggggaca accagaaagt ttcaagcgac aggctcgcgc ccggctggag aggaggacgc 180gggcggcccg gagcggcccg gggacgtggt gaacgtggtg ttcgtagacc gctcaggcca 240gcggatccca gtgagtggca gagtcgggga caatgttctt cacctggccc agcgccacgg 300ggtggacctg gaaggggcct gtgaagcctc cctggcctgc tccacctgcc atgtgtatgt 360gagtgaagac cacctggatc tcctgcctcc tcccgaggag agggaagacg acatgctaga 420catggccccc ctcctccagg agaactcgcg gctgggctgc cagattgtgc tgacaccgga 480gctggaagga gcggaattca ccctgcccaa gatcaccagg aacttctacg tggatggcca 540tgtccccaag ccccactgac atgaacacct ggaccattcc acattgccat ggccccaggg 600cccagattga gggaatagcc aggtgccagc cctgcccaga gtgcggacag gcccgggaga 660gacgtggaag cccctgtgaa ggacaacacc cctgcttggg agagagtccc atgtccaggc 720tctggtgggg acagggcccc tagtggggtg gccttcccca ggcccctgag aatcagggtt 780tgagtaggag tggactcata ttggagctgc aataaatcga taacacagga aaaaaaaaaa 840267606DNAHomo sapiensmisc_featureIncyte Clone No 2986229 267aataatgttt gagacagaag agaccattgg ctagtattta gcaattatca tagttatttg 60atttatatta aaaagcattt gtctttccac taaaacataa agggaataag ggcctagagt 120tatatgagtt aatagtaatt atagtcaagc tggggttaaa aatttgttgt agatgatgca 180tacttgggga taattaagag taccatctaa ttttctgtca ctttagaaag gaacaagtgg 240caactttgtt gactatgtgg agaaagccag atgttcttta ctcagtaata cctgttactt 300ctcttttttt ccttttagca ctgaacctac cagatgtatt tgggttggtc gtcctcccat 360tggaactgaa actacggatc ttccgacttc tggatgttcg ttccgtcttg tctttgtctg 420cggtttgtcg tgacctcttt actgcttcaa atgacccact cctgtggagg tttttatatc 480tgcgtgattt tcgaggtgat ttccgtaatg acatattcac aagaaagggc tcttattgtc 540ttgattactc agctcaccaa aagtttttag ttgtaggatt tttctgttgc aaatgattac 600aataaa 6062681025DNAHomo sapiensmisc_featureIncyte Clone No 3222081 268gtccttcgag ctactccgtc tggccccgcc ttttctctgc tctcctgaac ctttaggctt 60gtctcggccc atttgaagac caggaagttg atcaatcccg aggctgctga gagacggtgg 120cgcgattggg acagtcgcca gggatggctg agcgtgaaga tgcagcgggt gtccgggctg 180ctctcctgga cgctgagcag agtcctgtgg ctctccggcc tctctgagcc gggagctgcc 240cggcagcccc ggatcatgga agagaaagcg ctagaggttt atgatttgat tagaactatc 300cgggacccag aaaagcccaa tactttagaa gaactggaag tggtctcgga aagttgtgtg 360gaagttcagg agataaatga agaagaatat ctggttatta tcaggttcac gccaacagta 420cctcattgct ctttggcgac tcttattggg ctgtgcttaa gagtaaaact tcagcgatgt 480ttaccattta aacataagtt ggaaatctac atttctgaag gaacccactc aacagaagaa 540gacatcaata agcagataaa tgacaaagag cgagtggcag ctgcaatgga aaaccccaac 600ttacgggaaa ttgtggaaca gtgtgtcctt gaacctgact gatagctgtt ttaagagcca 660ctggcctgta attgtttgat atatttgttt aaactctttg tataatgtca gagactcatg 720tttaatacat aggtgatttg tacctcagag cattttttaa aggattcttt ccaagcgaga 780tttaattata aggtagtacc taatttgttc aatgtataac attctcagga tttgtaacac 840ttaaatgatc agacagaata atattttcta gttattatgt gtaagatgag ttgctatttt 900tctgatgctc attctgatac aactattttt cgtgtcaaat atctactgtg cccaaatgta 960ctcaatttaa atcattactc tgtaaaataa ataagcagat gattcttata atgaaaaaaa 1020aaaaa 10252696PRTArtificial SequenceDescription of Artificial Sequence Synthetic 6xHis tag 269His His His His His His 1 5

Claims

1. An isolated polynucleotide encoding a polypeptide comprising an amino acid sequence having at least about 95% sequence identity to an amino acid sequence of SEQ ID NO: 43, wherein the homologous polypeptide has a silent change in its amino acid sequence relative to the polypeptide represented by SEQ ID NO: 43.

2. The isolated polynucleotide of claim 1, wherein the polynucleotide encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 43.

3. The isolated polynucleotide of claim 1, comprising a polynucleotide sequence of SEQ ID NO: 177.

4. A recombinant polynucleotide comprising a promoter sequence operably linked to the polynucleotide of claim 1.

5. An isolated cell transformed with the recombinant polynucleotide of claim 4.

6. A method of producing a polypeptide comprising: a) culturing a cell under conditions suitable for expression of the polypeptide, wherein the cell is transformed with a recombinant polynucleotide, and the recombinant polynucleotide comprises a promoter sequence operably linked to the polynucleotide of claim 1, and b) recovering the polypeptide so expressed.

7. The method of claim 6, wherein the recombinant polynucleotide comprises the polynucleotide sequence of SEQ ID NO: 177.

8. An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of: a) a polynucleotide sequence having at least about 95% sequence identity to a polynucleotide sequence of SEQ ID NO: 177, wherein the homologous polynucleotide encodes a polypeptide with at least one functional characteristic of the polypeptide encoded by the polynucleotide sequence of SEQ ID NO: 177; b) a polynucleotide sequence complementary to the polynucleotide sequence of a); and c) an RNA equivalent of the polynucleotide sequence of a) or b).

9. The isolated polynucleotide of claim 8, comprising the polynucleotide sequence of SEQ ID NO: 177.

10. An isolated polypeptide selected from the group consisting of: a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 43; b) a polypeptide comprising a naturally occurring amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO: 43; c) a biologically active fragment of a polypeptide having the amino acid sequence of SEQ ID NO: 43; and d) an immunogenic fragment of a polypeptide having the amino acid sequence of SEQ ID NO: 43.

11. The isolated polypeptide of claim 10, comprising a naturally occurring amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO: 43.

12. The isolated polypeptide of claim 10, comprising an amino acid sequence of SEQ ID NO: 43.