NEW PHYTOPHTHORA RESISTANCE GENE

Abstract

The invention relates to a new gene that produces a protein which is capable of inferring oomycete resistance, more preferably resistance to Phytophthora infestans when expressed in a plant, wherein said nucleotide sequence encodes a protein that is encoded by the protein produced by the nucleotide sequence of FIG. 10 or the protein depicted in FIG. 11 or a nucleotide sequence that codes for a protein that has an identity of at least 95% with said protein produced by the nucleotide sequence of FIG. 10 or the protein depicted in FIG. 11. The invention also relates to a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with a nucleotide sequence as indicated above or a functional fragment thereof, preferably wherein said plant is a plant from the Solanaceae family, more preferably Solanum tuberosum.

Description

[0001] This application is a national phase entry pursuant to 35 U.S.C. .sctn.371 of International Application No. PCT/NL2015/050646, filed Sep. 17, 2015, which claims the benefit of U.S. Provisional Application No. 62/051,361, filed Sep. 17, 2014.

[0002] This application contains a sequence listing submitted in electronic format. The file name is "2017-10-23_01190-0001-00US_seqlist_ST25," it was created on Oct. 23, 2017, and is 269,566 bytes in size.

FIELD OF THE INVENTION

[0003] The invention is related to new resistance genes to oomycete infections, in particular infections with Phytophthora infestans in plants, in particular potato plants. Further part of the invention are the production of transgenic plants with said resistance gene and transgenic resistant plants harbouring said genes.

BACKGROUND

[0004] Potato is the third largest global food crop after wheat and rice and it suffers from yield losses up to 16% because of infestations with late blight.

[0005] Late blight, caused by the oomycete Phytophthora infestans, is one of the most serious diseases in worldwide potato production. It was responsible for the Irish potato famine of the mid-19th century, resulting in the death of one million people. Although a lot of effort has been invested in controlling the pathogen, chemical control of P. infestans is still the main crop management strategy, but environmental safety is becoming more important and the pathogen is sometimes able to evolve resistance to the fungicide treatment. Therefore, introduction of resistance into modern potato varieties is the most durable strategy to control the disease.

[0006] The family of Solanaceae is of high economic importance and is composed of more than 3,000 species which include important crop and model plants such as potato (Solanum tuberosum), tomato (Solanum lycopersicum) and eggplant (Solanum melongena) (Knapp, S., 2002, J. Exp. Bot. 53:2001-2022), but also wild species occurring in very different habitats (Spooner, D. M. and Hijmans, R. J., 2001, Amer. J. Potato Res. 78:237-268). About 15,000 wild potato accessions are being maintained in large collections worldwide and the establishment of core and mini collections enables an effective use of the existing variation in gene banks while maintaining the variability, as has been proposed before (Hoekstra, R. 2009, Potato Res. 52:237-244). Plant breeders try to improve varieties by introducing new alleles, resulting in higher yields and better quality or resistance characteristics. Identifying new, promising alleles is not an easy task. In the post-genomics era, mining of a crop's (wild) gene pool for novel and superior alleles for agronomically important traits is becoming more and more feasible. Genebanks all over the world contain huge untapped resources of distinct alleles that may have potential application in crop breeding programs. The genome sequence of potato (Potato Genome Sequencing Consortium 2011) and tomato (The Tomato Genome Consortium 2012) will facilitate mining for novel alleles or paralogs of resistance (R) genes. These may be found in the largely untapped resources of crossable species within the genus Solanum allowing their exploitation in breeding programs. Also, insight into sequence diversity at the R gene loci in wild Solanum species with different resistance response against economically important diseases will result in a better understanding of the mechanism of R gene functionality and evolution but can also help to identify new alleles or paralogs with different race specificities, and develop allele-specific diagnostic markers for marker assisted breeding.

[0007] In the last century, Solanum demissum, which is a hexaploid Mexican species, was extensively used in breeding for late-blight resistance in potato. Initially, a series of 11 R genes derived from S. demissum was described. Of these, R1, R2, R3a/b, R6, and R7 have been localized on the genetic maps of potato (Solanum tuberosum). However, these R genes confer pathovar-specific resistance and those that were introgressed into potato varieties, mainly R1, R2, R3, R4, and R10 (Vleeshouwers, V. G. A. A. et al., 2011, Ann. Rev. Phytopathol. 49:507-531), were quickly overcome by the pathogen. Also several other wild Solanum species have been reported as being potential sources of resistance, many of which have been genetically characterized (Table 1). Recent efforts to identify late blight resistance have focused on major R genes conferring broad-spectrum resistance derived from diverse wild Solanum species. Beside S. demissum, other wild Solanum species such as S. acaule, S. chacoense, S. berthaultii, S. breuidens, S. bulbocastanum, S. microdontum, S. sparsipilum, S. spegazzinii, S., stoloniferum, S. sucrense, S. toralapanum, S. uernei and S. uerrucosum have been reported as new sources for resistance to late blight (reviewed by Jansky S., 2000, Plant Breeding Rev. 19:69-155).

TABLE-US-00001 TABLE 1 R-genes and quantitative trait loci for late blight resistance reported for wild Solanum species Locus type Also known Wild species or name as Chromosome cloned Reference S. berthaultii QTLs (4) I, III, VII and XI Rpi-ber X (Rauscher et al., 2006) Rpi-ber1 X (Park et al.) Rpi-ber2 X (Park et al.) S. bulbocastanum RB/Rpi-blb1 RB VIII yes (Song et al., 2003; van der Vossen et al., 2003) Rpi-blb2 VI yes Van der Vossen et al. 2005 Rpi-blb3 IV yes (Park et al., 2005a) S. caripense QTL (2) unassigned S. demissum R1 V yes (Ballvora et al., 2002) R2 IV yes (Park et al., 2005b) R3, R6, R7 XI R3a XI yes (Huang et al., 2005) R3b XI R5-R11 XI R10, R11 XI (Bradshaw et al., 2006) S. microdontum QTLs (3) IV, V and X (Tan et al., 2008) QTL Unassigned S. mochiquense Rpi-mcq1 (Rpi-moc1) IX yes S. papita Rpi-pta1 VIII yes (Vleeshouwers et al., 2008) S. paucissectum QTLs (3) X, XI and XII S. phureja Rpi-phu1 IX S. pinnatisectum Rpi-pnt1 (Rpi1) VII (Kuhl et al., 2001) S. stoloniferum Rpi-sto1 VIII yes (Wang et al., 2008) S. venturii Rpi-vnt1.1 Rpi-phu1 IX yes Foster et al. 2009 Rpi-vnt1.3 IX yes Pel et al. 2009 S. vernei QTLs VI, VIII, IX (several) Hybrids with Rpi-abpt IV yes Lokosou et al. 2009 S. tuberosum R2-like IV yes (Park et al., 2005b)

Recently, a further R gene from S. demissum, which was denominated R8 was mapped in more detail (Jo, K.-R. et al., 2011, Theor. Appl. Genet. 123:1331-1340). This resistance gene has been labeled as providing a durable resistance, also because infections with P. infestans isolates that were derived from clonal lineage USB, which is recognized as the most common and the most aggressive genotype of P. infestans in the US (Fry. W. E and Goodwin, S. B., 1997, Bioscience 47:363-367), were overcome by the R8 producing plants, both in detached leaf assays and in field trials (Bisognin, D. A. et al., 2002, Euphytica 125:129-138).

[0008] In 2009, the sequence of the P. infestans genome of .about.240 Mb size has been published (Haas B. J. et al., 2009, Nature 461:393-398). The genome of P. infestans revealed large complex families of effector genes encoding secreted proteins involving pathogenesis which fall into two broad categories of apoplastic effectors and cytoplasmic effectors (Dodds P. N. and Rathjen, J. P., 2010, Nat. Rev. Genet. 11:539-548). The former accumulate in the plant intercellular space (apoplast) and include secreted hydrolytic enzymes such as proteases, lipases and glycosylases, enzyme inhibitors to protect against host defence enzymes, and necrotizing toxins. The latter are translocated directly into the plant cell by specialized infection structures known as haustoria (Whisson S. C. et al., 2007, Nature 450:115-118). 563 RXLR and 196 Crinkler (CRN) cytoplasmic effectors have been revealed by annotation of the P. infestans genome (Haas et al. 2009). The domain structure of P. infestans AVR proteins shows a typical modular structure with a N-terminal (signal peptide) domain, RXLR motif (Arg-X-Leu-Arg, where X indicates any amino acid), and C-terminal effector domain that often contains conserved amino acids residues (W, Y, and L) and tandem repeats (Oliva, R. et al., 2010, Cell Microbiol. 12:705-715; Schomack, S. et al., 2010, Proc. Natl. Acad. Sci. USA 107:17421-17426; Win, J. et al., 2012, PLoS Pathogens 8(1):e1002400). The N-terminal domain plays a role in secretion and host translocation whereas the variable C-terminal domain carries the effector biochemical activity. Like RXLRs, CRNs are modular proteins. CRNs are defined by a highly conserved N-terminal, 50-amino-acid LFLAK domain and an adjacent diversified DWL domain followed by a diverse C-terminal domain (Haas et al. 2009).

[0009] The Avr genes reside in the gene sparse regions with bigger distance to their neighboring genes (Haas et al. 2009) and represent the highly variable peripheral genome. To date, a catalog of more than eight Rpi and Avr gene pairs for the potato-P. infestans pathosystem is available, including R1/Avr1 (Ballvora, A. et al., 2002, Plant J. 30:361-371), R2/Avr2 (Lokossou, A. A. 2010, PhD thesis, Wageningen University; Champouret, N. 2010, PhD thesis, Wageningen University), R3a/Avr3a (Huang, S. et al., 2005, Plant J. 42:251-261; Armstrong, M. R. et al., 2005, Proc. Natl. Acad. Sci. USA 102:7766-7771), R3b/Avr3b (Li, G. et al., 2011, Mol. Plant Microbe Interact. 24(10):1132-1142, R4/Avr4 (van Poppel, P. M. J. A. et al., 2008, Mol. Plant Microbe Interact. 21:1460-1470), Rpi-blb1/Avrblb1 (van der Vossen, E. A. G. et al. 2003, Plant J. 23:567-576; Vleeshouwers, V. G. G. A. et al. 2008, PLoS ONE 3:e2875), Rpi-blb2/Avrblb2 (van der Vossen, E. A. G. et al., 2005, Plant J. 44:208-222; Oh, S. K. et al., 2009, Plant Cell 21:2928-2947), and Rpi-vnt1/Avrvnt1 (Foster, S. J. et al., 2009, Mol. Plant Microbe Interact. 22:589-600; Pel, M. A. 2010, PhD thesis, Wageningen University), which have proven valuable in e.g., dissecting resistance in genetically modified plants (Zhu, S. X. et al., 2012, Transgenic Res. 21:89-99) and classical breeding material (Rietman, H. et al., 2012, Mol. Plant Microbe Interact. 25:910-919).

[0010] Field resistance against late blight, which occurs in several potato varieties has been thought to have its basis on other mechanisms than R genes, since the field resistance has a more durable nature, while R genes are quickly defeated because of the ability of P. infestans to quickly adapt and evolve and break through such a `qualitative` resistance. This has led to the misguided concept of breeding for so-called `R gene free` potato plants that carry field resistance but lack known R genes. However, the genetic basis of field resistance has remained unclear, mainly because the weak phenotypes are too difficult to follow in the genetically complex potato and because hitherto the AVR profiles of infecting P. infestans strains could not be determined accurately.

[0011] "Sarpo Mira" is one of the few potato cultivars that have been reported to retain resistance in the field for several years and it is a candidate for delivering durable late blight resistance (Kim, H. J. et al., 2011, Theor. Appl. Genet. 124:923-935). The cultivar was bred by the Sarvari family in Eastern Hungary. However, to our knowledge, the pedigree is not described in the literature. Most likely, prebreeding leaned on the heritage of the Russian breeders Vavilov and Bukasov. Unfortunately, the origin or genetic constituents that determine the resistance of `Sarpo Mira` are not known, and the degree to which resistance includes previously characterized R genes could not be unambiguously determined using classical pathogen assays. To elucidate this further, Rietman, H. et al. (2012, Mol. Plant-Microbe Interact. 25(7):910-919) have found that the resistance has a highly complex genetic basis. They found that it is based on the combination of four pyramided qualitative R genes, including the `old` R3a, R3b and R4 resistance genes (see table 1) and a newly discovered gene, labelled Rpi-Smira1, and a quantitative resistance conferred by a novel gene, Rpi-Smira2. While the qualitative resistance matched responses to avirulence (AVR)3a (PITG_14371), AVR3b (PITG_18215), AVR4 (PITG_07387) and AVRSmira1 (PITG_07750) RXLR effectors, which was overcome by particular P. infestans strains, the quantitative resistance surprisingly appeared to be corresponding to responses to the RXLR effector AvrSmira2 (PITG_07758) and was only detectable under field conditions. However, Rietman et al. did not elucidate the molecular nature of the Rpi-Smira2 gene or its location on the genome of the `Sarpo Mira` variety. Further, they argue that the quantitative resistance is only effective in combination with a qualitative resistance, and that in the case of `Sarpo Mira` the five resistance genes act in concert to provide the durable field resistance.

[0012] Accordingly, there still lacks a gene that is able to provide field resistance against late blight and methods to provide such a durable resistance to plants.

SUMMARY OF THE INVENTION

[0013] The inventors now have found the R8 gene from Solanum demissum and show that it is the same gene as the putative Rpi-Smira2 gene found in potato variety `Sarpo Mira`.

LEGEND TO THE FIGURES

[0014] FIG. 1. Positions of NBS profiling markers and R gene homologs on chromosome IX.

[0015] Markers in large font indicate the NBS profiling markers that were linked to R8. Potato genome sequences, the tomato genome sequences and the marker sequence database from the SGN were searched using the NBS5a6H and NBS1M by BLAST analysis. The bars on the left indicate S. phureja scaffolds (PGSC v3). In the middle are the tomato EXPEN 2000 genetic map and the tomato SL2.40 Ch9 physical map (SGN). On the right positions of tomato genome sequences with homology to R genes are shown. Horizontal and diagonal lines indicate corresponding marker positions in the different maps.

[0016] FIG. 2. Comparison of different genetic maps of chromosome IX. From left to right the potato SH.times.RH map (Van Os et al. 2006), the R8 map produced in this study and the combined Solanaceae pathogen resistance map as extracted from the GABI website (May 20, 2011). Only the long arms of chromosome IX are shown. The dotted arrows indicate relative positions of studied markers shared between the different maps.

[0017] FIG. 3. Agroinfiltration assay for parents MaR8 and cv Concurrent and nine resistant and nine susceptible progeny. R-Avr interactions were validated by co-segregation of responses to the effector AVR8 with resistance to P. infestans isolate IPO-C in F1 population (MaR8.times.Concurrent). Avr8 was transiently expressed (left side) in MaR8, Concurrent and F1 progeny plants by agroinfiltration. A 1:1 mixture of R3a and Avr3a was infiltrated as positive controls (right side).

[0018] FIG. 4. Avr8 effector features. A. Avr8-containing genomic region. The 1620-1900 kb of genomic region in supercontig 1.11 was shown. This region includes AvrSmira1 (PITG_07550), PITG_07555, PITG_07556, PITG_07566. The Avr8 gene is present in P. infestans and P. mirabilis but not in P. ipomoeae and P. phaseoli. B. The Avr8 gene is a single copy gene and encodes a secreted protein with a signal peptide followed by a typical RXLR motif and most likely an EER-like motif at the N-terminal (SEQ ID NO: 39) and a WY domain at the C-terminal effector region (SEQ ID NO: 40). C. A heat map showing expression levels of five RXLRs including the Avr8 gene from a time course infection on potato (6 to 120 hpi). Out of these RXLR effectors, PITG_07550, PITG_07555, PITG_07556, PITG_07566, Avr8 (PITG_07558) is the only one specifically induced at 6/16 hpi. my; mycelia, sp; sporangia, zo; zoospores. Gene induction is relative to mycelia as previously reported by Raffaele et al. (2010b). The gene expression values are from the reference genome strain T30-4 (Haas et al. 2009).

[0019] FIG. 5. Rpi-Smira2 resides in R8 locus on chromosome IX. The quantitative resistance conferred by Rpi-Smira2 in cv. Sarpo Mira based on AVR8 responsiveness (AVR8-HR) mapped between R8 flanking markers 184-81 and CDP4 where R8 was also localized (Jo et al. 2011). The map distances were calculated based on the frequency of the recombination between markers.

[0020] FIG. 6. AVR8-responding Solanum germplasm accessions are distributed both in Central and South America. The phylogenetic tree was assembled using the AFLP fingerprinting data generated by Jacobs et al. (2008). All fingerprints can be obtained from SolRgene database website. Some genotypes, e.g., TUQ299-4 (previously named DMS299-4), DMS585-7, and CHN544-2 (previously named STO554-2), were not included in the phylogenetic tree because these are not available in SolRgene database. Genotypes in red color respond to Avr8. At the right side is a geographical overview of Avr8-responding Solanum species. The bubble size represents number of genotypes tested, and red sector the number of AVR8 responding genotypes. DMS; S. demissum, TAR; S. tarijense, GIG; S. microdontum gigantophyllum, USA; United States, MEX; Mexico, GTM; Guatemala, COL; Colombia, ECU; Ecuador, PER; Peru, BOL; Bolivia, ARG; Argentina. The unclassified Solanum, previously named S. astleyi and S. ugentii were not included.

[0021] FIG. 7. Genetic and Physical map of R8 on the bottom end of chromosome 9.

[0022] The upper part of this figure represents a genetic map. Markers of different origin were mapped in a F1 recombinant population (R8*C) of 1670 individuals and their relative positions are indicated by vertical lines. The number of recombinants between the markers are indicated by numbers between the vertical lines. The horizontal line marked by R8 indicates the genetic mapping interval for R8. The lower part of this figure represents a physical map. Using molecular markers BAC clones were identified from BAC libraries derived from MaR8. The grey bars represent the individual BAC clones. Vertical lines indicate the connection between physical and genetic maps. The arrows on the bottom line indicate the position and orientations of resistance gene analogs (RGAs) on the physical map, which are labeled as RGA0.10-RGA3.3.

[0023] FIG. 8: Nucleotide sequence of the 170 kb BAC contig (SEQ ID NO: 38).

[0024] FIG. 9. R8 complementation analysis. A. Transgenic Desiree events, transformed with resistance gene analogs (RGAs) show different phenotypes after inoculation of whole plants with IPO-C, ranging from full resistance (R), to intermediate resistance (IR), to susceptible (S). B. Co-expression of RGA0.20, and not of any of the other RGAs (RGA0.21 is here provided as an example), with different concentrations (OD=1, 0.5, or 0.25) of Avr8 in N. benthamiana results in a HR response.

[0025] FIG. 10. The nucleotide sequence of the R8 gene (SEQ ID NO: 36). The coding sequence is in bold (SEQ ID NO: 37). Start and stopcodons are underlined. The coding sequence is preceded by the 5'UTR region and followed by the 3'UTR sequence. A Thymine at position -723 in the 5'UTR of R8 differed from Rpi-smira2 which had a Cytosine residue at this position. This SNP is underlined and in Italics.

[0026] FIG. 11. Amino acid sequence architecture of the R8 protein. (SEQ ID NO: 37) Coiled coil (CC), Nucleotide binding Apaf-1 R gene and CED4 homology (NB-ARC), and leucine rich repeat (LRR) regions can be distinguished. Conserved residues are in underlined red font.

[0027] FIG. 12. Phylogenetic analysis of R8 and other R proteins from Solanaceae. The column on the right of the protein names contains the genetic location of the encoding genes (chromosome, and RGA cluster number; Vossen et al. 2013). Clades observed in this tree match the sequence groups defined previously (extreme right column; Andolfo et al. 2014).

[0028] FIG. 13. R8 mediated broad spectrum resistance in field trials in 2014. Bintje spreader rows were derived from seed tubers. Desiree and Desiree transformants were planted from in vitro culture. At the onset of the natural late blight epidemic the Bintje plants were much taller than the Desiree and R8 transgenics (Desiree:R8). This explains the difference in the height of the deceased Bintje and Desiree plants, while the R8 transgenics kept growing.

DETAILED DESCRIPTION

[0029] As used herein, the term "plant or part thereof" means any complete or partial plant, single cells and cell tissues such as plant cells that are intact in plants, cell clumps and tissue cultures from which potato plants can be regenerated. Examples of plant parts include, but are not limited to, single cells and tissues from pollen, ovules, leaves, embryos, roots, root tips, anthers, flowers, fruits, stems shoots, tubers, including potato tubers for consumption or `seed tubers` for cultivation or clonal propagation, and seeds; as well as pollen, ovules, leaves, embryos, roots, root tips, anthers, flowers, fruits, stems, shoots, scions, rootstocks, seeds, protoplasts, calli, and the like.

[0030] As used herein, the term "population" means a genetically heterogeneous collection of plants sharing a common genetic derivation.

[0031] As used herein, the term "variety" is as defined in the UPOV treaty and refers to any plant grouping within a single botanical taxon of the lowest known rank, which grouping can be: (a) defined by the expression of the characteristics that results from a given genotype or combination of genotypes, (b) distinguished from any other plant grouping by the expression of at least one of the said characteristics, and (c) considered as a unit with regard to its suitability for being propagated unchanged.

[0032] The term "cultivar" (for cultivated variety) as used herein is defined as a variety that is not normally found in nature but that has been cultivated by humans, i.e. having a biological status other than a "wild" status, which "wild" status indicates the original non-cultivated, or natural state of a plant or accession. The term "cultivar" specifically relates to a potato plant having a ploidy level that is tetraploid. The term "cultivar" further includes, but is not limited to, semi-natural, semi-wild, weedy, traditional cultivar, landrace, breeding material, research material, breeder's line, synthetic population, hybrid, founder stock/base population, inbred line (parent of hybrid cultivar), segregating population, mutant/genetic stock, and advanced/improved cultivar.

[0033] As used herein, "crossing" means the fertilization of female plants (or gametes) by male plants (or gametes). The term "gamete" refers to the haploid or diploid reproductive cell (egg or sperm) produced in plants by meiosis, or by first or second restitution, or double reduction from a gametophyte and involved in sexual reproduction, during which two gametes of opposite sex fuse to form a diploid or polyploid zygote. The term generally includes reference to a pollen (including the sperm cell) and an ovule (including the ovum). "Crossing" therefore generally refers to the fertilization of ovules of one individual with pollen from another individual, whereas "selfing" refers to the fertilization of ovules of an individual with pollen from genetically the same individual.

[0034] The term "backcrossing" as used herein means the process wherein the plant resulting from a cross between two parental lines is crossed with one of its parental lines, wherein the parental line used in the backcross is referred to as the recurrent parent. Repeated backcrossing results in the genome becoming more and more similar to the recurrent parent, as far as this can be achieved given the level of homo- or heterozygosity of said parent.

[0035] As used herein, "selfing" is defined as refers to the process of self-fertilization wherein an individual is pollinated or fertilized with its own pollen.

[0036] The term "marker" as used herein means any indicator that is used in methods for inferring differences in characteristics of genomic sequences. Examples of such indicators are restriction fragment length polymorphism (RFLP) markers, amplified fragment length polymorphism (AFLP) markers, single nucleotide polymorphisms (SNPs), insertion mutations, microsatellite markers (SSRs), sequence-characterized amplified regions (SCARs), cleaved amplified polymorphic sequence (CAPS) markers or isozyme markers or combinations of the markers described herein which defines a specific genetic and chromosomal location.

[0037] As used herein, "locus" is defined as the genetic or physical position that a given gene occupies on a chromosome of a plant.

[0038] The term "allele(s)" as used herein means any of one or more alternative forms of a gene, all of which alleles relate to the presence or absence of a particular phenotypic trait or characteristic in a plant. In a diploid cell or organism, the two alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes. It is in some instance more accurate to refer to "haplotypes" (i.e. an allele of a chromosomal segment) in stead of "allele", however, in these instances, the term "allele" should be understood to comprise the term "haplotype".

[0039] The term "heterozygous" as used herein, and confined to diploids, means a genetic condition existing when different alleles reside at corresponding loci on homologous chromosomes.

[0040] As used herein, and confined to diploids, "homozygous" is defined as a genetic condition existing when identical alleles reside at corresponding loci on homologous chromosomes.

[0041] As used herein, and confined to tetraploids, the term "nulliplex", "simplex", "duplex", "triplex" and "quadruplex", is defined as a genetic condition existing when a specific allele at a corresponding locus on corresponding homologous chromosomes is present 0, 1, 2, 3 or 4 times, respectively. At the tetraploid level the phenotypic effect associated with a recessive allele is only observed when the allele is present in quadruplex condition, whereas the phenotypic effect associated with a dominant allele is already observed when the allele is present in a simplex or higher condition.

[0042] The terms "haploid", "diploid" and "tetraploid" as used herein are defined as having respectively one, two and four pairs of each chromosome in each cell (excluding reproductive cells).

[0043] The term "haplotype" as used herein means a combination of alleles at multiple loci that are transmitted together on the same chromosome. This includes haplotypes referring to as few as two loci, and haplotypes referring to an entire chromosome depending on the number of recombination events that have occurred between a given set of loci.

[0044] As used herein, the term "infer" or "inferring", when used in reference to assessing the presence of the fungal resistance as related to the expression of the R8 gene, means drawing a conclusion about the presence of said gene in a plant or part thereof using a process of analyzing individually or in combination nucleotide occurrence(s) of said gene in a nucleic acid sample of the plant or part thereof. As disclosed herein, the nucleotide occurrence(s) can be identified directly by examining the qualitative differences or quantitative differences in expression levels of nucleic acid molecules, or indirectly by examining (the expression level of) a the R8 protein.

[0045] The term "primer" as used herein refers to an oligonucleotide which is capable of annealing to the amplification target allowing a DNA polymerase to attach thereby serving as a point of initiation of DNA synthesis when placed under conditions in which synthesis of primer extension product which is complementary to a nucleic acid strand is induced, i.e., in the presence of nucleotides and an agent for polymerization such as DNA polymerase and at a suitable temperature and pH. The (amplification) primer is preferably single stranded for maximum efficiency in amplification. Preferably, the primer is an oligodeoxyribonucleotide. The primer must be sufficiently long to prime the synthesis of extension products in the presence of the agent for polymerization. The exact lengths of the primers will depend on many factors, including temperature and source of primer. A "pair of bi-directional primers" as used herein refers to one forward and one reverse primer as commonly used in the art of DNA amplification such as in PCR amplification.

[0046] As used herein, the term "probe" means a single-stranded oligonucleotide sequence that will recognize and form a hydrogen-bonded duplex with a complementary sequence in a target nucleic acid sequence or its cDNA derivative.

[0047] The terms "stringency" or "stringent hybridization conditions" refer to hybridization conditions that affect the stability of hybrids, e.g., temperature, salt concentration, pH, formamide concentration and the like. These conditions are empirically optimised to maximize specific binding and minimize non-specific binding of primer or probe to its target nucleic acid sequence. The terms as used include reference to conditions under which a probe or primer will hybridise to its target sequence, to a detectably greater degree than other sequences (e.g. at least 2-fold over background). Stringent conditions are sequence dependent and will be different in different circumstances. Longer sequences hybridise specifically at higher temperatures. Generally, stringent conditions are selected to be about 5.degree. C. lower than the thermal melting point (I'm) for the specific sequence at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength and pH) at which 50% of a complementary target sequence hybridises to a perfectly matched probe or primer.

[0048] Typically, stringent conditions will be those in which the salt concentration is less than about 1.0 M Na+ ion, typically about 0.01 to 1.0 M Na+ ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30.degree. C. for short probes or primers (e.g. 10 to 50 nucleotides) and at least about 60.degree. C. for long probes or primers (e.g. greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide. Exemplary low stringent conditions or "conditions of reduced stringency" include hybridization with a buffer solution of 30% formamide, 1 M NaCl, 1% SDS at 37.degree. C. and a wash in 2.times.SSC at 40.degree. C. Exemplary high stringency conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37.degree. C., and a wash in 0.1.times.SSC at 60.degree. C. Hybridization procedures are well known in the art and are described in e.g. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K. eds. (1998) Current protocols in molecular biology. V. B. Chanda, series ed. New York: John Wiley & Sons.

[0049] The present invention describes the cloning of the R8 gene (also identified as Rpi-Smira2 gene). R8 was mapped to a new R gene locus on chromosome IX using a S. demissum MaR8 mapping population. The R gene locus contains at least 10 paralogous sequences, that are closely related to the R8 gene. Only 1 of the six tested paralogs provided recognition of the P. infestans Avr8 effector protein. The R8 gene codes for a protein which is denominated as R8 and the protein encoding nucleotide and its 5' and 3' UTR sequences may be found in FIG. 10 of the present application. As can be derived from Table 8, outside the R gene cluster described in this invention, R8 does not share high identities with known R genes that are known to be specific for Phytophthora. The R8 gene and its paralogs share, however, a high identity with a gene from tomato, that is known to provide resistance to tomato spotted wilt virus (TSWV). This Sw-5 protein and R8 share 88.6% homology. Within genes that are known or have been reported to confer resistance against P. infestans, the highest amino acid sequence identity (26.1%) is to a protein Rpi-blb2 derived from Solanum bulbocastanum (Van de Vossen et al., 2005, The Plant Journal 44, 208-222).

[0050] In a first embodiment, the invention provides an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding the R8 amino acid sequence as encoded by the nucleic acid presented in FIG. 10 or a functional fragment thereof, i.e. a functional fragment of the amino sequence as encoded by the nucleotide sequence of FIG. 10. Further encompassed in the present invention are sequences that code for a protein that has more than 95% identity of the R8 protein. More preferably, the nucleic acid encoding for such a protein has at least 95% identity with the nucleotide sequence presented in FIG. 10 with respect to the coding part.

[0051] The term "nucleic acid" means a single or double stranded DNA or RNA molecule.

[0052] Also included are the complementary sequences of the herein described nucleotide sequences.

[0053] The term "functional fragment thereof" is typically used to refer to a fragment of the R8 protein that is capable of providing at least partial resistance or increasing resistance in a plant of the Solanaceae family against an oomycete infection. Such a fragment is, for example, a truncated version of the R8 protein as presented in FIG. 10. A truncated version/fragment of the R8 protein is a fragment that is smaller than 1245 amino acids and preferably comprises part of the LRR domain (i.e. part of the leucine-rich repeats domain which stretches from about amino acid 896 to amino acid 1245 of R8 and/or the N-terminal parts of the R8 protein.

[0054] Also included are protein sequences that are highly homologous to or have a high identity with the herein described R8 protein, where such proteins are capable of providing at least partial resistance or increasing resistance in a plant of the Solanaceae family against an oomycete infection. Included are artificial changes or amino acid residue substitutions that at least partly maintain the effect of the R8 protein. Preferably such proteins would maintain the ability to recognize the P. infestans Avr8 effector protein and induce a defense response. Such proteins are highly homologous or have a high identity with the herein described R8 protein, for example, certain amino acid residues of the original R8 protein can conventionally be replaced by others of comparable nature, e.g. a basic residue by another basic residue, an acidic residue by another acidic residue, a hydrophobic residue by another hydrophobic residue, and so on. Examples of hydrophobic amino acids are valine, leucine and isoleucine. Phenylalanine, tyrosine and tryptophan are examples of amino acids with an aromatic side chain and cysteine as well as methionine are examples of amino acids with sulphur-containing side chains. Serine and threonine contain aliphatic hydroxyl groups and are considered to be hydrophilic. Aspartic acid and glutamic acid are examples of amino acids with an acidic side chain. In short, the term "highly homologous" or "having a high identity" include variants of the R8 protein in which amino acids have been inserted, replaced or deleted and which at least partly maintain the effect of the R8 protein (i.e. at least partly providing or increasing resistance in a plant of the Solanaceae family against an oomycete infection). Preferred variants are variants which only contain conventional amino acid replacements as described above. A high identity in the definition as mentioned above means an identity of at least 95%. Most preferred are amino acids that have an identity of 96, 97, 98 or 99% with the amino acid sequence of R8.

[0055] Also included are nucleic acid sequences that encode a highly homologous protein as described above. Preferred are nucleotide sequences that have a high identity with respect to their nucleic acid sequence with the nucleic acid sequence depicted in FIG. 12, at least the coding portion of the nucleic acid sequence of FIG. 12.

[0056] Homology and/or identity percentages can for example be determined after sequence alignments with computer programs such as BLAST, ClustalW or ClustalX. Preferably, for the purposes of the present invention, identity of proteins s determined by the alignment method labeled ClustalW found in the MegAlign.TM. v6.1 program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Default parameters for multiple alignment correspond to GAP PENALTY=10, GAP LENGTH PENALTY=0.2, Delay Divergen Seqs (%)=30, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB. After alignment of the sequences using the ClustalW program, it is possible to obtain a "percent identity" by viewing the "sequence distances" table in the same program.

[0057] For nucleotide sequences the BLASTN method is used, which alignment is an algorithm provided by the National Center for Biotechnology Information (NCBI) to compare nucleotide sequences using default parameters.

[0058] Many nucleic acid sequences code for a protein that is 100% identical to the R8 protein as presented in FIG. 13. This is because nucleotides in a nucleotide triplet may vary without changing the corresponding amino acid (wobble in the nucleotide triplets). Thus, without having an effect on the amino acid sequence of a protein the nucleotide sequence coding for this protein can be varied. However, in a preferred embodiment, the invention provides an isolated or recombinant nucleic acid sequence as depicted in FIG. 10. In a preferred embodiment, the invention provides an isolated, synthetic, or recombinant nucleic acid that represents the coding sequence (CDS) of the R8 protein, i.e. the bold nucleotides of FIG. 10 or a functional fragment thereof. For the purposes of this invention, this coding sequence may be combined with regulatory nucleotide sequences needed for a proper expression

[0059] A further preferred variant of the nucleic acid sequence as defined above is the nucleic acid sequence that is found in the Rpi-smira2 gene of the potato variety Sarpo Mira. This gene was retrieved from this potato variety by PCR amplification and successive cloning using R8 specific primers. The nucleotide sequence of this gene had one polymorphism when compared with the R8 gene (T>C at position -723 in the 5'UTR in R8 vs Rpi-smira2).

[0060] Fragments as well as highly homologous genes of the herein described R8 gene and protein can for example be tested for their functionality by using an Agrobacterium tumefaciens transient transformation assays (agro-infiltration) and/or by using a detached leaf assay as described in the experimental section.

[0061] The experimental part for example describes a functional screen for testing candidate genes using agroinfiltration, whereby 4 week old wild type Nicotiana benthamiana plants are infiltrated with Agrobacterium strains containing the candidate R8 homologues. The infiltrated leaves are subsequently challenged one day after infiltration with a P. infestans strain that is virulent on N. benthamiana, for example IPO-C or 90128, in detached leaf assays. This system is equally suitable for testing candidate homologous fragments of R8. A person skilled in the art thus can easily determine whether or not an R8 fragment can be considered to be a functional fragment.

[0062] Transient gene expression, as is achieved through agroinfiltration, is a fast, flexible and reproducible approach to high-level expression of useful proteins. In plants, recombinant strains of Agrobacterium tumefaciens can be used for transient expression of genes that have been inserted into the T-DNA region of the bacterial Ti plasmid. A bacterial culture is infiltrated into leaves, and upon T-DNA transfer, there is ectopic expression of the gene of interest in the plant cells. However, the utility of the system is limited because the ectopic RNA expression ceases after 2-3 days. It is shown that post-transcriptional gene silencing (PTGS) is a major cause for this lack of efficiency. A system based on co-expression of a viral-encoded suppressor of gene silencing, the p19 protein of tomato bushy stunt virus (TBSV), prevents the onset of PTGS in the infiltrated tissues and allows high level of transient expression. Expression of a range of proteins was enhanced 50-fold or more in the presence of p19 so that protein purification could be achieved from as little as 100 mg of infiltrated leaf material. Although it is clear that the use of p19 has advantages, an agroinfiltration without p19 can also be used to test the functionality of candidate fragments and functional homologues.

[0063] Alternatively, each candidate gene (for example being a fragment or homologue) construct is targeted for transformation to a susceptible potato cultivar, for example Desiree. Primary transformants are challenged in detached leaf assays using for example isolates IPO-0, IPO-C or 90128. Transformants that are resistant to these isolates harbour for example functional fragments or highly homologous sequences of R8.

[0064] In yet another embodiment, the invention provides a vector comprising a nucleic acid as provided herein, i.e. a nucleic acid capable of providing at least partial resistance or increasing resistance in a plant of the Solanaceae family against an oomycete infection. More particularly, the invention provides a vector comprising an isolated, synthetic or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding the amino acid sequence R8 of FIG. 13 or a functional fragment or a functional highly homologous sequence thereof. The invention also provides a vector comprising a nucleic acid sequence as depicted in FIG. 10 or as described above.

[0065] Examples of a suitable vector are pBeloBACII, pBINplus, pKGW-MG, or any commercially available cloning vector.

[0066] As will be outlined below there are multiple ways in which a nucleic acid of the invention can be transferred to a plant. One suitable means of transfer is mediated by Agrobacterium in which the nucleic acid to be transferred is part of a binary vector and hence it is preferred that the above described vector is a binary vector. Another suitable means is by crossing a plant which contains the gene encoding R8 to a plant that does not contain the gene and to identify those progeny of the cross that have inherited the R8 gene.

[0067] The invention further provides a host cell comprising a nucleic acid as described herein or a vector as described herein. Examples of a preferred host cell are an E. coli cell suitable for BAC clones (e.g. DH10B) or an Agrobacterium (host) cell. In another embodiment, said host cell comprises a plant cell. A preferred plant cell is a cell derived from a member of the Solanaceae family and even more preferred said plant cell comprises a cell from Solanum tuberosum, Solanum lycopersicum, formerly known as Lycopersicon esculentum, pepper and eggplant. From such a cell, a transgenic or genetically modified plant (for example a potato or tomato plant) can be obtained by methods known by the skilled person (for example regeneration protocols).

[0068] The invention further provides a leaf, tuber, fruit or seed or part or progeny of a genetically modified plant as described herein.

[0069] In yet another embodiment, the invention provides a protein encoded by the herein described isolated or recombinant nucleic acid or a functional fragment or a functional homologue thereof. In a preferred embodiment, the invention provides a protein encoded by a nucleic acid sequence as depicted in FIG. 10. In yet another preferred embodiment, the invention provides a protein comprising the amino acid sequence of FIG. 11 or a functional fragment or a highly homologous sequence thereof.

[0070] The herein described R8 protein comprises 1245 amino acids and the LRR domains of R8 consist of 13 imperfect repeats (FIG. 11). Interestingly, R8 shares the highest homology (>80%) with a gene from S. lycopersicon and not with R genes that are known to associate with P. infestans resistance (Table 9). Further, it appeared that of the 7 tested paralogs that were found in the cluster, of which R8 is one, only R8 could recognize the Avr8 protein of P. infestans. Further, as can be derived from table 7, the other paralogs were not effective in whole-plant assays and field assays. It can thus be concluded that it is surprising that a gene which has a very low homology (about 15-25%) with other known R genes against Phytophthora infestans infection is able to provide a high and during resistance in the field, while genes that are highly homologous with the R8 gene are not able to provide resistance.

[0071] As already described, a functional fragment or a functional highly homologous sequence of R8 is a fragment or homologue that is capable of providing at least partial resistance or increasing resistance in a plant of the Solanaceae family against an oomycete infection and/or recognition of the P. infestans Avr8 effector protein. This means that at least the 6 paralogs (RGA 1.0, 1.1, 1.2, 0.21, 3.1 and 3.2) that have been tested as described in the experimental part are not considered to be functional highly homologous sequences to R8.

[0072] Means to test the functionality of a functional fragment or a functional homologue of R8 have been provided above and may be found in some detail in the experimental section of the present application. Recognition of the Avr8 effector protein can be achieved according to methods as found in the experimental description.

[0073] Based on the herein described nucleic acid sequences, the invention also provides probes and primers (i.e. oligonucleotide sequences complementary to one of the (complementary) DNA strands as described herein). Probes are for example useful in Southern or northern analysis and primers are for example useful in PCR analysis. Primers based on the herein described nucleic acid sequences are very useful to assist plant breeders active in the field of classical breeding and/or breeding by genetic modification of the nucleic acid content of a plant (preferably said plant is a Solanum tuberosum, Solanum lycopersicum, formerly known as Lycopersicon esculentum), pepper or eggplant in selecting a plant that is capable of expressing for example R8 or a functional fragment or functional highly homologous sequence thereof.

[0074] Hence, in a further embodiment, the invention provides a binding molecule capable of binding to a nucleic acid encoding R8 or a functional fragment or functional highly homologous sequence thereof as described herein or its complementary nucleic acid. In a preferred embodiment, said binding molecule is a primer or a probe. As mentioned, such a binding molecule is very useful for plant breeders and hence the invention further provides a method for selecting a plant or plant material or progeny thereof for its susceptibility or resistance to an oomycete infection. Preferably, the nucleic acid of a plant to be tested is isolated from said plant and the obtained isolated nucleic acid is brought in contact with one or multiple (preferably different) binding molecule(s). One can for example use a PCR analysis to test plants for the presence of absence of R8 in the plant genome. Such a method would be especially preferable in marker-free transformation protocols, such as described in WO 03/010319.

[0075] The herein described R8 protein can also be used to elicit antibodies by means known to the skilled person. The invention thus also provides an antibody that (specifically) binds to the protein encoded by the herein described isolated or recombinant nucleic acid (for example the nucleic acid sequence of FIG. 10) or an antibody that (specifically) binds to a protein as depicted in FIG. 11 or a functional fragment or a functional homolog thereof. Such an antibody is for example useful in protein analysis methods such as Western blotting or ELISA, and hence can be used in selecting plants that successfully express the R8 gene.

[0076] Based on the herein provided nucleic acid sequences, the invention also provides the means to introduce or increase resistance against an oomycete infection in a plant. The invention therefore also provides a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with:

[0077] an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding the R8 amino acid sequence of FIG. 13 or a functional fragment or a functional highly homologous sequence thereof, or

[0078] an isolated or recombinant nucleic acid sequence as depicted in FIG. 10, or

[0079] a vector comprising the herein described nucleic acid sequences, or

[0080] a host cell as described herein.

[0081] Such a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection may be based on classical breeding, departing from a parent plant that already contains the R8 gene or a functional homolog thereof, or it involves the transfer of DNA into a plant, i.e., involves a method for transforming a plant cell comprising providing said plant cell with a nucleic acid as described herein or a vector as described herein or a host cell as described herein.

[0082] There are multiple ways in which a recombinant nucleic acid can be transferred to a plant cell, for example Agrobacterium mediated transformation. However, besides by Agrobacterium infection, there are other means to effectively deliver DNA to recipient plant cells when one wishes to practice the invention. Suitable methods for delivering DNA to plant cells are believed to include virtually any method by which DNA can be introduced into a cell, such as by direct delivery of DNA such as by PEG-mediated transformation of protoplasts, by desiccation/inhibition-mediated DNA uptake (Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985), by electroporation (U.S. Pat. No. 5,384,253), by agitation with silicon carbide fibers (Kaeppler et al., 1990; U.S. Pat. No. 5,302,523; and U.S. Pat. No. 5,464,765), and by acceleration of DNA coated particles (U.S. Pat. No. 5,550,318; U.S. Pat. No. 5,538,877; and U.S. Pat. No. 5,538,880). Through the application of techniques such as these, cells from virtually any plant species may be stably transformed, and these cells may be developed into transgenic plants.

[0083] In case Agrobacterium mediated transfer is used, it is preferred to use a substantially virulent Agrobacterium such as A. tumefaciens, as exemplified by strain A281 or a strain derived thereof or another virulent strain available in the art. These Agrobacterium strains carry a DNA region originating from the virulence region of the Ti plasmid pTiBo542, which coordinates the processing of the T-DNA and its transfer into plant cells. Agrobacterium-based plant transformation is well known in the art (as e.g. described in, for example by Komari, T. et al.: Plant Transformation Technology: Agrobacterium-Mediated Transformation, in: Handbook of Plant Biotechnology, Eds. Christou, P. and Klee, H., John Wiley & Sons, Ltd, Chichester, UK, 2004, pp. 233-262). Preferably a marker-free transformation protocol is used, such as described in WO03/010319.

[0084] Alternatively, the nucleic acid of the R8 gene or a functional highly homologous sequence thereof may be introduced into a plant by crossing. Such a crossing scheme starts off with the selection of a suitable parent plant. This may for instance be an original Solanum demissum variety or a plant from the S. tuberosum variety `Sarpo Mira` or a plant that has obtained the desired nucleic acid by genetic engineering as described above.

[0085] Any suitable method known in the art for crossing selected plants may be applied in the method according to the invention. This includes both in vivo and in vitro methods. A person skilled in the art will appreciate that in vitro techniques such as protoplast fusion or embryo rescue may be applied when deemed suitable.

[0086] Selected plants that are used for crossing purposes in the methods according to the invention may have any type of ploidy. For example, selected plants may be haploid, diploid or tetraploid. Most cultured potato plants are diploid, and preferably tetraploid. Solanum demissum is a hexaploid plant. Crossing a hexaploid plant with a diploid or a tetraploid plant will generally result in offspring that is sterile.

[0087] Thus, when plants are selected that are hexaploid, their ploidy must be decreased to diploid or tetraploid level before they can be crossed with another diploid or tetraploid plant in the methods according to the invention. Methods for decreasing the ploidy of a plant are well known in the art and can be readily applied by a person skilled in the art. For example, ploidy of a diploid plant for crossing purposes can be increased by using 2N gametes of said diploid plant. Ploidy can also be increased by inhibiting chromosome segregation during meiosis, for example by treating a diploid plant with colchicine. By applying such methods on a diploid plant, embryos or gametes are obtained that comprise double the usual number of chromosomes. Such embryos or gametes can then be used for crossing purposes. For potatoes a resistant tetraploid plant is preferred, since tetraploid plants are known to have higher yields of tubers. A decrease in ploidy can for instance be achieved by a meiotic division and parthenogenesis from said meiotically divided cell. It is also possible to cross hexaploid cells with diploid cells to obtain (in general a low yield of) tetraploid offspring.

[0088] Since the resistance characteristic has appeared to be a dominant trait, it is sufficient if only one allele with the functional gene is present.

[0089] Preferably, selected plants are crossed with each other using classical in vivo crossing methods that comprise one or more crossing steps including selfing. By applying such classical crossing steps characteristics of both the parents can be combined in the progeny. For example, a plant that provides a high yield can be crossed with a plant that contains large amounts of a certain nutrient. Such a crossing would provide progeny comprising both characteristics, i.e. plants that not only comprise large amounts of the nutrient but also provide high yields.

[0090] When applying backcrossing, F1 progeny is crossed with one of its high-yielding parents P to ensure that the characteristics of the F2 progeny resemble those of the high-yielding parent. For example, a selected diploid potato with oomycete resistance is made tetraploid by using colchicine and then crossed with a selected high-yielding tetraploid potato cultivar, with the purpose of ultimately providing a high-yielding tetraploid progeny having oomycete resistance. Also selfing may be applied. Selected plants, either parent or progeny, are then crossed with themselves to produce inbred varieties for breeding. For example, selected specimens from the above mentioned F1 progeny are crossed with themselves to provide an F2 progeny from which specimens can be selected that have an increased level of resistance.

[0091] It is also possible to use a potato breeding technology as described in WO 2011/053135 and WO 2012/144902.

[0092] After transfer of a nucleic acid into a plant or plant cell, it must be determined which plants or plant cells have been provided with said nucleic acid. When selecting and crossing a parental genotype in a method according to the invention, a marker is used to assist selection in at least one selection step. It is known in the art that markers, indicative for a certain trait or condition, can be found in vivo and in vitro at different biological levels. For example, markers can be found at peptide level or at gene level. At gene level, a marker can be detected at RNA level or DNA level. Preferably, in the present invention the presence of such a marker is detected at DNA level, using the above described primers and/or probes. Alternatively, proper expression of the R8 protein or a functional homolog thereof can be assessed in plant parts by performing an immunoassay with an antibody that specifically binds the protein. Next to the primers and probes according to the invention, use can also be made of specific markers that are to be found in the vicinity of the coding sequence. Such markers are indicated in the experimental part below and comprise for instance the markers At5-2FspBI and 184-81-Rsa1, and especially the markers 10-SCAR and 993-DraI (see FIG. 7).

[0093] In case of transgenic approaches selecting a transformed plant may be accomplished by using a selectable marker or a reporter gene. Among the selective markers or selection genes that are most widely used in plant transformation are the bacterial neomycin phosphotransferase genes (nptI, nptII and nptIII genes) conferring resistance to the selective agent kanamycin, suggested in EP131623 and the bacterial aphIV gene suggested in EP186425 conferring resistance to hygromycin. EP 275957 discloses the use of an acetyl transferase gene from Streptomyces viridochromogenes that confers resistance to the herbicide phosphinotricin. Plant genes conferring relative resistance to the herbicide glyphosate are suggested in EP218571. Suitable examples of reporter genes are beta-glucuronidase (GUS), beta-galactosidase, luciferase and green fluorescent protein (GFP).

[0094] In a preferred embodiment, the invention provides a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with:

[0095] an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding the R8 amino acid sequence of FIG. 13 or a functional fragment or functional highly homologous sequence thereof, or

[0096] an isolated or recombinant nucleic acid sequence as depicted in FIG. 10 comprising at least the coding part of said sequence,

[0097] a vector comprising the herein described nucleic acid sequences, or

[0098] a host cell as described herein,

[0099] wherein said oomycete comprises Phytophthora, preferably Phytophthora infestans and/or wherein said plant comprises a plant from the Solanaceae family, preferably a potato or tomato plant, more preferably a tetraploid potato plant.

[0100] In a further preferred embodiment, the invention comprises introduction into a plant cell or plant of an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding the R8 amino acid sequence of FIG. 11 or a functional fragment or functional highly homologous sequence thereof and next to this nucleic acid another nucleic acid encoding a resistance gene that provides protection against oomycete infection, in particular infection with Phytophthora, more particularly P. infestans. Preferably such other resistance genes are selected from Table 1, and preferably selected from the genes Rpi-chc1, Rpi-ber, Rpi-sto1, Rpi-blb3, Rpi-edn2, Rpi-vnt1.1 and Rpi-blb2. Most preferred are Rpi-edn2, Rpi-vnt1.1 and Rpi-blb2. The invention also provides a plant that is obtainable by using a method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection as described above. A preferred plant is a plant from the Solanaceae family and even more preferred said plant is a Solanum tuberosum or a Solanum lycopersicum, formerly known as Lycopersicon esculentum, Solanum melononga, Capsicum spp., such as C. annuum, C. baccatum, C. chinense, C. frutescens and C. pubescens. The invention thus also provides a plant that has been provided with a nucleic acid encoding a R8 protein or a functional fragment or a functional homologue thereof.

[0101] The invention further provides a plant part or progeny of a plant according to the invention comprising a nucleic acid encoding the R8 amino acid sequence of FIG. 13 or a functional fragment or a functional highly homologous sequence thereof.

[0102] In a preferred embodiment, the herein described nucleic acid is transferred to a Solanum variety other than Solanum demissum, i.e. the herein described nucleic acid is preferably provided to a non-demissum background, preferably S. lycopersicon or S. tuberosum. Of the latter most preferred is a tetraploid variety and more preferably to a commercial interesting variety such as Bintje, Desiree or Premiere, Spunta, Nicola, Favorit, Russet Burbank, Aveka or Lady Rosetta.

[0103] It is also possible to provide the resistance according to the invention to a plant that is already partially resistant to an oomycete infection, wherein said plant is provided with a nucleic acid encoding a further resistance gene, such as R3a, R3b, R4, Rpi-chc1, Rpi-blb1, -2, -3, Rpi-vnt1 or Rpi-mcq1.

[0104] The invention further provides use of an isolated or recombinant nucleic acid sequence comprising a nucleic acid sequence encoding the R8 amino acid sequence of FIG. 11 or a functional fragment or a functional highly homologous sequence thereof or use of an isolated or recombinant nucleic acid sequence as depicted in FIG. 10 or use of a vector comprising any of said nucleic acid sequences or use of a host cell comprising any of said nucleic acid sequences or said vector for providing a plant with at least partial resistance against an oomycete infection. In a preferred embodiment, said oomycete comprises Phytophthora and even more preferably Phytophthora infestans. In yet another preferred embodiment said plant comprises Solanum tuberosum or Solanum lycopersicum, formerly known as Lycopersicon esculentum.

[0105] In yet another embodiment, the invention provides a method for producing R8 protein or a functional fragment or a functional homologue thereof comprising functionally linking a nucleic acid as described herein to a regulatory sequence and allowing said nucleic acid to be expressed in a host cell. Examples of a regulatory sequence are a promoter and/or terminator sequence. Further, the R8 sequence may be expressed under control of its own promoter and terminator, but also other strong or inducible promoters and or vectors may be provided. Therefore, the invention further provides the promoter and/or terminator sequences of R8 (FIG. 10). FIG. 10 shows the nucleotide sequence of R8 (7011 bp) containing the R8 gene and regulatory sequences. The R8 coding region of 3735 bp is in bold font. The upstream nucleotides (nt 1-1679) and the downstream nucleotides harbour the regulatory sequences that ensure correct expression of the gene. The skilled person is very well capable of cloning (part of) said regulatory sequences and testing their efficiency in transcription. It has further been found that even a better expression is obtained with a truncated promoter, i.e. a promoter containing less than 1000, preferably not more than 900 base pairs upstream of the gene sequence.

[0106] The invention will be explained in more detail in the following, non-limiting examples.

EXPERIMENTAL PART

Example 1: Mapping of S. demissum Late Blight Resistance Gene R8

[0107] Plant Material and Mapping Population

[0108] MaR8, corresponding to 2424a(5) and PI 303149 (Black et al. 1953; Malcolmson and Black 1966), and cultivar Concurrent were maintained and in vitro and multiplied in the Laboratory of Plant Breeding, Wageningen University. MaR8, as resistant female parent, and the susceptible cultivar Concurrent were crossed to generate a F1 mapping population in the summer of 2008 (population code R8*C). Seeds were sown under sterile conditions and 100 plants were maintained in in vitro culture.

[0109] Phytophthora infestans Isolates and Disease Testing

[0110] Phytophthora infestans isolate IPO-C (race 1, 2, 3, 4, 5, 6, 7, 10, 11) was kindly provided by Prof. Francine Govers (Laboratory of Phytopathology, Wageningen University). IPO-C was used in detached leaf assays as described by Vleeshouwers et al. (1999) but also to inoculate field trials. In 2009 and 2010, respectively, four and two in vitro plants per genotype from population R8*C were planted in the beginning of June. Spreader rows and the border rows consisted of the susceptible potato cultivars Bintje and Nicola, which served to support a local late blight epidemic. In the beginning of July, the trial fields were inoculated. For the inoculum production, a large number of detached leaves of potato cultivar Bintje were inoculated with isolate IPO-C. After 6 days, spores were washed off to prepare a spore suspension in large containers. Zoospore release was induced by incubating the containers at 10.degree. C. After the release of the zoospores, the inoculum was adjusted to a concentration of 5.times.10.sup.4 zoospores/ml. At nightfall, the zoospore suspension was sprayed on the potato field using a tractor using a spraying arm. After 2 weeks severe late blight symptoms were observed in susceptible plants and a clear segregation of resistance and susceptibility was observed in population R8*C. Scoring was performed in a qualitative way (resistant or susceptible).

[0111] DNA Isolation and Marker Analysis

[0112] Genomic DNA was isolated as described by Fulton et al. (1995). Young leaf tissue was collected for DNA isolation according to the CTAB protocol with the Retsch machine (RETSCH Inc., Hannover, Germany). Primers used for marker analysis are listed in Table 2. PCR reactions were performed using DreamTaq.TM. polymerase (Fermentas) in a standard PCR program (start: 94.degree. C. for 30 s; amplification: 35 cycles of 94.degree. C. for 30 s, 55.degree. C. for 30 s; 72.degree. C. for 1 min; termination: 72.degree. C. for 2 min). NBS profiling was performed as described by Van der Linden et al. (2004), with minor modifications. The restriction enzyme digestion of genomic DNA and the ligation of adapters were made in one incubation step. Restriction enzymes MseI, HaeIII and RsaI were used for restriction ligation reactions and NBS primers NBS1, NBS2, NBS3, NBS5a6 and NBS9 in combination with the adaptor primer were used for the successive PCR reactions. Primers with corresponding names and sequences have been described previously (Van der Linden et al. 2004; Wang et al. 2008; Mantovani et al. 2006; Brugmans et al. 2008). Totally, 15 primer enzyme combinations were used for NBS profiling. For R gene CDP, R2 and Tm-22 primers (R2LF1, R2LF2, R2LF3, R2LF4, R2LR2, R2LR3, R2LR4, Tm1F, Tm1R, Tm3F, Tm3R, Tm6F, Tm15F, Tm15R, Tm19F, Tm19R, Mcq19F, Mcq21R and Mcq23F) were used as described by Verzaux (2010). HotStarTaq.TM. polymerase (QIAGEN) was used in the first PCR and DreamTaq.TM. polymerase (Fermentas) in a second PCR. For designing Hero-CDP-primers, Hero-like sequences available from NCBI (www.ncbi.nlm.nih.gov) and S. phureja DM1-3 516R44 (CIP801092) whole genome assembly scaffold sv3 available from the Potato Genome Sequencing Consortium (PGSC; www.potatogenome.net) were collected and aligned. Primers were designed on cluster specific conserved domains encoding CC and LRR. A total of six Hero-CDP degenerate primers were designed and one produced a marker that was linked to R8 (Table 2). For Sw-5-CDP seven specific primers described by Dianese et al. (2010) were used. Like in NBS profiling, the CDP primers were used in combination with a labeled adaptor primer (fluorescent dye IRD700) to enable visualization on a denaturing polyacrylamide gel using a NEN_IR2 DNA analyser (LI-COR_Biosciences, Lincoln, Nebr., USA). NBS profiling was carried out first on a set of 10 resistant and 10 susceptible F1 plants, including the parents. If in this first round polymorphic bands between the parents and co-segregation of these bands with resistance in the F1 plants were found, a second round of NBS profiling was carried out on genomic DNA of the remaining F1 progeny. If multiple markers are found with one primer/enzyme combination, numbers behind the dash are consecutive numbers ordered from low to high molecular weight produced by the same primer enzyme combination. For example, marker CDPTm21-1 and CDPTm21-2 were produced using primer/enzyme combination Tm15R/MseI. In order to screen for cleaved amplified polymorphic sequences (CAPS), PCR was done using primers listed in Table 2 and successively the PCR products were digested using the restriction enzymes listed in Table 2. 5 .mu.l of PCR product were added to a 15 .mu.l of restriction enzyme digestion according to the manufacturers' instructions.

TABLE-US-00002 TABLE 2 Markers used for mapping of R8 in the F1 population MaR8 x Concurrent SEQ ID Poly- Marker name Primer name Primer sequence (5'.fwdarw.3')* NO morphism Reference NBS5a6H NBS5a6 YYTKRTHGTMITKGATGAYRTITGG 1 HaeIII Van der Linden et al. 2004 NBS1 M NBS1 GCIARWGTWGTYTTICCYRAICC 2 MseI CDP.sup.Tm21-1 Tm15R GTAACAAGTCATGTATGCGAC 3 MseI Verzaux 2010 CDP.sup.Tm21-2 Tm15R GTAACAAGTCATGTATGCGAC 3 MseI Verzaux 2010 CDP.sup.Tm22 Tm19F GCCAAATAGTATTGTCAAGCTC 4 MseI Verzaux 2010 CDP.sup.Hero3 Hero4064F RRAGATTCAGCCATKGARATTAAGAAA 5 HaeIII This study CDP.sup.Sw54 Sw55F AGTCTCCAAACATTCCTGCTTCTC 6 MseI Dianese et al. 2010 CDP.sup.Sw55 Sw55F AGTCTCCAAACATTCCTGCTTCTC 6 HaeIII Dianese et al. 2010 Stm1021 STM1021 F GGAGTCAAAGTTTGCTCACATC 7 SSR Collins etal. 1999 STM1021 R CACCCTCAACCCCCATATC 8 TG328 TG328F TGAATGGACTGGTGATCTGC 9 SCAR This study TG328R TTGGAAAGAATTGGCTTTTGA 10 184-81 184-81F CCACCGTATGCTCCGCCGTC 11 CAPS, RsaI This study 184-81R GTTCCACTTAGCCTTGTCTTGCTCA 12 General primers for profiling MseI adaptor Mse-ad-top CCCGAAAGTATAGATCCCAT 13 Van der Linden et al. 2004 Mse-ad- TAATGGGATCTATACTT 14 bottom Blunt adaptor B-ad-top ACTCGATTCTCAACCCGAAAGTATAGATCCCA 15 Van der Linden et al. 2004 B-ad-bottom TGGGATCTATACTT 16 Adaptor ACTCGATTCTCAACCCGAAAG 17 Van der Linden et al. 2004 primer F; forward primer, R; reverse primer. *All markers were produced using a standard PCR program

Isolation and Sequence Analysis of NBS Fragments

[0113] Fragments were excised as described in the Odyssey manual for band extraction (Westburg, The Netherlands) and re-amplified with the specific profiling primer and the adapter primer. PCR products were checked on agarose gels and purified with QIAquick PCR purification spin columns (QIAGEN Benelux, The Netherlands). Fragments were cloned into the pGEM-T Easy vector (Promega, USA) prior to sequencing with M13 primers. Sequencing was carried out with the BigDye Terminator kit and an ABI 3700 automated sequencer from Applied Biosystems (USA). Blast analysis of the sequences was performed using the websites from NCBI, PGCS and SGN (blast.ncbi.nlm.nih.gov/; potatogenomics.plantbiology.msu.edu/; solgenomics.net). ClustalX (Jeanmougin et al. 1998) was used to align sequences.

[0114] Map Construction

[0115] Co-segregating, simplex-inherited NBS and CDP markers from the tetraploid female parent (MaR8) were scored as dominant markers (Wu et al. 1992). The marker order was determined by TetraploidMap (Hackett and Luo 2003; www.bioss.ac.uk). The map distance was calculated based on the frequency of the recombination between markers. Publicly available potato and tomato genetic maps from the SH.times.RH population (Van Os et al. 2006), SGN sgn.cornell.edu/cview/map.pl?map_id=9&show_offsets=1&show_ruler=1) and GABI (www.gabipd.org/database/) databases were included for comparison of marker positions and synteny.

[0116] Results

[0117] Segregation of Resistance in the Mapping Population

[0118] F1 progeny and the parental clones MaR8 and cv. Concurrent were screened for resistance against P. infestans isolate IPO-C. The detached leaf assay with leaves from greenhouse grown plants turned out not to be suitable for the F1 population. In contrast to the mother plant MaR8, the F1 plants showed no clear resistance. Initial screens indicated some variation in resistance; however, these findings were not reproducible for most of the individuals. In contrast, highly reproducible results were obtained in two field trials performed in Wageningen, The Netherlands, in the summer of 2009 and 2010. MaR8 plants remained devoid of late blight symptoms, while cv. Concurrent was completely infected within 2 weeks after inoculation. Among 100 F1 genotypes screened, 52 were resistant, 46 were susceptible and 2 showed intermediate phenotypes. This demonstrates that the resistance in MaR8 is inherited as a dominant simplex allele (X.sup.2=0.54, P>0.05) at a single locus. The corresponding gene is referred to as R8 hereafter.

[0119] Identification of R8 Flanking Markers

[0120] In order to identify markers linked to R8, we used NBS profiling since this technique can also give an indication about the R gene family of the targeted gene. Initially, NBS profiling experiments were carried out using combinations of the NBS5a6 primer and three enzymes (HaeIII, RsaI and MseI) on both parents and 10 resistant and 10 susceptible F1 individuals from the mapping population. Marker NBS5a6H was linked to the resistance phenotype and was found at a frequency of one recombinant in twenty F1 plants. Subsequently, an additional set of NBS primers (NBS1, NBS2, NBS3 and NBS9) was used which resulted in the identification of an additional marker, NBS1M showing linkage to the resistance but without recombinants in twenty F1 plants. The NBS5a6H and NBS1M markers were tested on the complete F1 progeny. 22 additional recombinants were found between NBS5a6H and R8, and three recombinants were identified between NBS1M and R8. These recombinants were not overlapping resulting in 26 recombinants between NBS1M and NBS5a6. This showed that the two NBS profiling markers flank the R8 gene (FIG. 1).

[0121] Localization of R8 Flanking Markers in the Genome

[0122] The NBS5a6H (361 bp) and NBS1M (301 bp) fragments were cut out of the gel and sequenced (genbank accession numbers: JF317286 and JF317287 respectively). In potato scaffold PGSC0003DMS000000483, a 93% identity match was found for the NBS5a6H sequence. PGSC0003DMS000000483 could be located to chromosome IX using genetic and physical maps of tomato (FIG. 1). NBS1M showed 97% identity to potato scaffold PGSC0003DM S000001347. This scaffold could be linked to the telomeric region at the long arm of chromosome IX using markers C2_At1g09815 and C2_At3g24160 (FIG. 1). The proposed inversions between potato and tomato on chromosome IX (Tanksley et al. 1992) did not affect the positioning of the R8 flanking markers. For marker NBS1M, there was no similarity to sequences with known function. The sequence of marker NBS5a6H, however, showed 90% identity to the tomato Hero gene (Ernst et al. 2002), which is located on chromosome IV. Apparently, Hero-like genes are not only present on chromosome IV but are located in other genomic regions as well (FIG. 1).

[0123] Localisation of R8 on Chromosome IX

[0124] In order to verify that R8 and its flanking markers were on chromosome IX, more closely linked markers near the R8 gene were required. Therefore, R gene CDP was performed. Two R gene clusters known to locate on chromosome IV (R2 and Hero), and two clusters known to locate on chromosome IX (Tm-22 and Sw-5) were targeted for R gene-CDP. Using R2-CDP no bands linked to the resistance were found among 24 primer/enzyme combinations (data not shown). Three linked markers, CDPTm21-1 (240 bp), CDPTm21-2 (345 bp) and CDPTm22 (120 bp), were identified using Tm-22 primers out of 36 primer/enzyme combinations. CDPTm21-1 and CDPTm21-2 were identified using the same primer enzyme combination (Tm15R/MseI). All Tm-22-CDP markers are at 2 cM distance (proximal) from R8 (FIG. 2). Two markers, CDPSw54 (277 bp) and CDPSw55 (165 bp), were identified using Sw-5-CDP. Both markers were located at 1 cM to the opposite side (distal) of the R8 gene as CDPTm21-1, CDPTm21-2 and CDPTm22 (FIG. 2). Interestingly, one fully co-segregating marker, CDPHero3 (500 bp), was found using Hero-CDP out of 18 primer/enzyme combinations. All CDP markers were excised from the gel and subjected to sequence analysis. CDPTm21-1 and CDPTm22 indeed showed similarity to Tm-22. CDPSw54 and CDPSw55 were confirmed to be similar to Sw-5, a S. lycopersicon tospovirus resistance gene (Brommonschenkel and Tanksley 1997; Spassova et al. 2001). Unfortunately, the sequences of CDPTm21-2 and CDP3 remained unresolved due to technical reasons. The relative positions of the Tm-22 and Sw-5 homologous markers in the R8 map are in agreement with relative positions of Rpi-moc1, which is homologous to Tm-22 (Foster et al. 2009) and Sw-5, as inferred from publically available genetic maps of chromosome IX (FIG. 2). In addition, the draft sequence of the complete tomato chromosome IX shows that Tm-22 and Sw-5 like sequences are located close to each other near the telomere (FIG. 1). To further confirm the map position of R8 and the newly identified profiling markers on chromosome IX, known markers (GP101, S2g3, TG591A, GP41, CT220, T0521, S1d11, S1d5-a, T1065, TG328, TG424, and St_At3g23400) from the SGN and GABI databases on the long arm of chromosome IX were selected and tested for linked polymorphisms after digestion with 24 selected restriction enzymes. Only TG328 did display an informative SCAR type polymorphism. A segregation of 87 presence to 12 absence genotypes was found which fits a 5:1 ratio (X.sup.2=0.23, p>0.05), indicating that the TG328 marker allele is present in duplex in MaR8. Also three SSR markers (Stm1010, Stm1021, Stm0017) (Milbourne et al. 1998; Collins et al. 1999) were screened and one SSR marker, Stm1021, present in RH9 BIN65 of the SH.times.RH map located at 9 cM proximal to R8. Since no other useful polymorphisms could be found in known genetic markers in this region, we mined for potential polymorphic regions in the potato genome covering this region. TG328 located to SH9 BIN77 of the SH.times.RH map, was linked to Rpi-moc1 in the GABI map, and located 2 cM proximal relative to R8 (FIG. 2). Scaffold PGSC0003DM S000000184 which contained the flanking markers TG328, CDPTm21-1 and CDPTm22, was aligned to the tomato genome and several polymorphic regions were identified. PCR screens within these regions eventually identified additional polymorphic marker (184-81), which located 1 cM proximal to R8 (FIG. 2).

Example 2: The Avirulence Factor AVR8 for the Mexican S. demissum Late Blight Resistance Gene R8 is Recognized by Cv. Sarpo Mira and Resistant Wild Solanum Species from South America

[0125] Materials and Methods

[0126] Plant Material

[0127] The potato differential plant MaR8, also known as 2424a(5), was used as female parent in a cross with the susceptible cultivar Concurrent to generate a F1 mapping population (R8*C). Cultivar Sarpo Mira was crossed with the susceptible clone RH89-039-16, a donor of the potato genome sequence, to produce population SM*RH. A hundred or thirty of genotypes of populations R8*C or SM*RH, respectively, and their parents, were clonally maintained in vitro culture containing Murashige and Skoog medium (Murashige and Skoog, 1962) supplemented with 3% (w/v) sucrose at 20.degree. C. The wild Solanum plant materials which were maintained in vitro at Wageningen University, Laboratory of Plant Breeding, were used for effector screening and functional allele mining. Information on species, origin, collection site, GPS coordinates and other genbank code are accessible on the SolRgene database.

[0128] Phytophthora infestans Isolate and Blight Resistance Tests

[0129] Phytophthora infestans isolate IPO-C (race 1, 2, 3, 4, 5, 6, 7, 10, 11) was used in field trials. Field trials for the mapping populations were done in 2009, 2010 and 2011 in Wageningen, The Netherlands, as described in previous studies (Jo et al. 2011; Rietman et al. 2012). Field trials for germplasm accessions were performed in the growing seasons of the years 2005 and 2007 in Wageningen, The Netherlands (Vleeshouwers et al. 2011b). Disease assessments were made in four replicates per genotype by estimating the percentage of leaf area covered with late blight lesions at multiple time points after inoculation. From these readings, the AUDPC was calculated (Fry 1978) and the AUDPC values were transformed to 1 (susceptible)-9 (resistant) scale.

[0130] Screening of in Planta-Induced RXLR Effectors

[0131] A genome-wide collection of RXLR effectors was selected from the P. infestans genome sequence based on presence of a predicted signal peptide, an RXLR motif, and an elevated gene expression at 6 hours post inoculation (hpi) to 3 days post inoculation (dpi) (Haas et al. 2009). Using Gateway.TM. technology, effectors were subcloned into pK7WG2 and transformed into Agrobacterium tumefaciens strain AGL1, pSoup, and pVirG cells by electroporation. Agroinfiltration was performed as previously described (Rietman et al. 2012). Briefly, A. tumefaciens strains from frozen glycerol stocks were grown overnight at 28.degree. C. in 3 ml of LB medium supplemented with appropriate antibiotics. The next day these cultures were used to inoculate 15 ml of YEB medium (5 g beef extract, 5 g bacteriological peptone, 5 g sucrose, 1 g yeast extract, 2 ml 1 M MgSO4 in 1 L of milli-Q water) supplemented with antibiotics, 10 .mu.l/L of 200 mM acetosyringone and 1000 .mu.l/L of 1M MES. On the third day, the cells were harvested and resuspended to a final OD600 of 0.3 in MMA (20 g sucrose, 5 g MS salts and 1.95 g MES in 1 liter of distilled water, adjusted to pH5.6 with KOH supplemented with 1 ml/L of 200 mM acetosyringone). Leaves of plants were infiltrated with this suspension. Two leaves per plant and three replicate plants of 4 to 5 weeks old were infiltrated with the following constructs: effectors, R3a (Huang et al. 2005) and Avr3a (Armstrong et al. 2005) as the positive control and empty pK7WG2 (Karimi et al. 2002) as the negative control. Responses were scored 3 to 4 days after infiltration.

[0132] DNA Isolation and Marker Analysis

[0133] Total genomic DNA was isolated from young leaves as described by Fulton et al. (1995). The Retsch homogenizer (RETSCH Inc., Hannover, Germany) was used to grind young plant materials frozen in liquid nitrogen. For mapping Avr8-responsiveness in F1 populations markers described by Jo et al. (2011) were used (Table 3). For the identification of markers associated with the recognition of AVR8 in various Solanum genotypes, a modification of the NBS profiling protocol of Van der Linden et al. (2004) was carried out as described in Jo et al. (2011). The concentrations of genomic DNA for all samples were adjusted to 300 ng/pi prior to profiling experiments. PCR reactions for markers 184-81 and Stm1021 were performed using the primers in Table 3 and DreamTaq.TM. polymerase (Fermentas) in a simple PCR program (94.degree. C. for 60 s followed by 30 cycles of 94.degree. C. for 30 s, 58.degree. C. for 60 s, 72.degree. C. for 90 s and a final extension time of 5 min at 72.degree. C.). The polymorphism for marker 184-81 was detected by digesting the PCR product with the restriction enzyme listed in Table 3 and 1% agarose gel electrophoresis. Marker Stm1021 is a simple sequence repeat marker. Polymorphisms were detected using polyacrylamide gel electrophoresis. Fragments were prepared using a labelled forward primer (fluorescent dye IRD800) to enable visualization on a denaturing polyacrylamide gel using a NENR IR2 DNA analyser (LI-CORR Biosciences, Lincoln, Nebr., USA).

TABLE-US-00003 TABLE 3 Markers used for mapping of Rpi-Smira2 in the F1 population (Sarpo Mira x RH) SEQ Tm Product Marker Primer Sequence (5'.fwdarw.3') ID NO Marker type (.degree. C.) size (bp) References CDP3 Hero4064F: 5 CDP/HaeIII 55 500 bp Jo et al. (2011) RRAGATTCAGCCATKGARATTAAGAAA 184-81 184-81F: CCACCGTATGCTCCGCCGTC 11 CAP SIRsaI 58 480 bp Jo et al. (2011) 184-81R: GTTCCACTTAGCCTTGTCTTGCTCA 12 CDP4 Sw55F: AGTCTCCAAACATTCCTGCTTCTC 6 CDP/MseI 55 277 bp Jo et al. (2011) Stm1021 Stm1021F: GGAGTCAAAGTTTGCTCACATC 7 SSR 58 210 bp Collins et al. (1999) Stm1021R: CACCCTCAACCCCCATATC 8 Sequences of adapter and adapter primer for CDP profiling MseI adapter Mse-ad-top: CCCGAAAGTATAGATCCCAT 13 Mse-ad-bottom: TAATGGGATCTATACTT 14 Blunt adapter B-ad-top: 15 van der Linden et al. ACTCGATTCTCAACCCGAAAGTATAGATCCCA (2004) B-ad-bottom: TGGGATCTATACTT 16 Adapter primer ACTCGATTCTCAACCCGAAAG 17

[0134] Map Construction

[0135] The marker order was determined by TetraploidMap (Hackett and Luo, 2003; www.bioss.ac.uk/knowledge/tetraploidmap/). The map distance was calculated based on the frequency of the recombination between markers.

[0136] Results

[0137] In Planta-Induced RXLR Effector Screening Reveals Candidate Genes for Avr8

[0138] MaR8 was functionally profiled for response to a collection of 234 predicted RXLR effectors selected from the Phytophthora infestans genome sequence described by Haas et al. (2009). Responses to effectors were quantitatively scored for the level of cell death, ranging from 0% (no symptoms) to 100% (confluent cell death in all replicates) four days after agroinfiltration. Out of the 234 tested effectors, 13 effectors triggered more than 30% of cell death in the MaR8 plant. Among those 13 effectors, four effectors, i.e., Avr3a,

[0139] Avr3b, Avr4 and AvrSmira2 (PITG_07558), were described previously to confer avirulence activity in Sarpo Mira (Rietman 2011).

[0140] Response to AVR8 is Co-Segregating with R8 Specific Resistance

[0141] To investigate which of the identified effectors had an avirulence function towards R8, we adopted a genetic approach. MaR8 was crossed with the susceptible cultivar Concurrent and 100 F1 genotypes (population R8*C) were assessed for resistance to P. infestans isolate IPO-C in detached leaf tests and in replicate field trials (Jo et al. 2011). This isolate is virulent on potatoes carrying R3a, R3b, and R4, and therefore, those R genes are expected not to interfere with the R8 phenotype. The population R8*C showed a clear segregation of 1:1 ratio for resistance and susceptibility in field trials but not in laboratory tests (data not shown). Subsequently, we tested this population for response to the effectors that were recognized in MaR8 (Table 4).

TABLE-US-00004 TABLE 4 RXLR effectors that trigger cell death response on the differential plant MaR8 Expression Effector .sup.a SignalP.sup.b in potato.sup.c Gene or construct Gene HMM NN mean 16 48 Response ID annotation Prob. NS score RXLR RXLR Tribe.sup.d hpi hpi MaR8(%).sup.e PITG_15039 0.993 0.928 RILV 1 0.83 1.12 100 PITG_22880 0.999 0.928 na 1 0.74 0.09 100 PITG_07558 Avr8 1.000 0.963 RSLR 2 1.11 0.24 95 PITG_04097 1.000 0.786 RSLR 5 1.40 1.28 75 PITG_18683 1.000 0.862 RSLR 5 -0.54 2.61 47 PITG_04169 0.995 0.918 RSLR 10 0.13 0.65 83 PITG_07387 Avr4 0.999 0.903 RFLR 52 0.03 2.00 100 PITG_10540 0.999 0.850 RFLR 57 -0.06 1.37 86 PITG_14371_KI.sup.f Avr3a (KI) na na RLLR 58 na na 100 PITG_14374 0.992 na RFLR 58 0.44 0.91 100 PITG_18215 Avr3b 0.999 0.857 RSLR 124 1.51 3.07 100 PITG_23129 0.956 0.452 RLLR 128 1.38 1.28 78 PITG_23131 0.983 0.831 RLLR 128 0.97 1.09 94 pMDC32 Neg control -- -- na na 0 R3a:Avr3a Pos control -- -- na na 100 .sup.a Description of predicted RXLR effectors from the P. infestans genome reference strain T30-4. .sup.bHidden Markov model (HMM) score and S-mean value predicted using SignalPv2.0. NN = neutral networks output, NS = S score (signal peptide score). .sup.cGene induction on potato in T30-4 reference genome strain. In planta-gene induction was estimated relative to the expression levels in mycelium. Hours post inoculation (hpi). .sup.dRXLR Tribe ID as described by Haas et al. (2009). .sup.ePercentage of cell death response upon agroinfiltration, based on an average of quantitative scores in at least nine replicates. .sup.fAVR3aKI is the avirulent allele of AVR3a. na; not assessed.

[0142] In an initial screen, agroinfiltration was done on ten resistant and ten susceptible individuals of population R8*C. Co-infiltration of R3a/Avr3a and the empty vector pK7WG2 were included as positive and negative controls, respectively. High levels of necrosis in the range of 30%-80% were observed in the negative control for five plants in the resistant set and six in the susceptible set. Also, the Concurrent parent displayed nonspecific response to agroinfiltration (FIG. 3). To establish a subset of the population that does not show nonspecific cell death to the negative control, we tested an additional 23 individuals of population R8*C for their responses to the empty vector pK7WG2 and R3a/Avr3a (co)infiltration. Altogether, nine resistant and nine susceptible plants showed no cell death to the negative control but did show cell death to the positive control. We subjected this mini-population to an agroinfiltration experiment with the Avr8 candidates in Table 4. The response to one effector PITG_07558 fully matched with the resistance to P. infestans isolate IPO-C in field trials (FIG. 3). To further confirm these co-segregation results on a large set of the R8*C population, 56 additional genotypes of population R8*C were tested. Despite the occurrence of certain levels of cell death responses to negative controls in five plants, for the remaining progeny clear distinction between PITG_07558-infiltrated spots (90-100%) and negative control spots (30-60%) was observed.

[0143] All 34 plants that responded to PITG_07558 were resistant to P. infestans in the field, whereas all 31 plants that failed to respond to PITG_07558 were susceptible. Thus, response to PITG_07558 fully co-segregated with R8 specific resistance to P. infestans and we designated PITG_07558 "Avr8". To test whether the response to Avr8 co-segregates with the presence of a R8 specific marker, we tested 65 genotypes which were used in agroinfiltration with marker CDP3 (Jo et al. 2011). All 34 resistant AVR8-responding plants contained CDP3, whereas all 31 susceptible non-responding plants did not. These data further confirm that the response to Avr8 is associated with R8 specific resistance.

[0144] Avr8 Gene Structure and Expression

[0145] Avr8 (PITG_07558) is a single copy gene in the T30-4 reference genome located at supercontig 11 (Haas et al. 2009; FIG. 4A). Indeed, the P. infestans transcripts database displayed only one expressed Avr8 copy (www.broadinstitute.org). Avr8 encodes a secreted protein with a typical RXLR effector of 244 amino acids and most likely an EER-like motif in its N-terminal domain and a WY-domain in its C-terminal domain (FIG. 4B). Similar to other P. infestans Avr genes, Avr8 resides in a gene-sparse repeat-rich region (GSR) of the P. infestans genome (FIG. 4A). The Avr8 gene is present in all thus far sequenced P. infestans isolates 90128, PIC99189, 06_3928A (13_A2), NL07434 and P17777 (US22) (Haas et al. 2009, Cooke et al. 2012 and unpublished), and no polymorphisms have been found thus far. Additional studies for genetic variation of Avr8 are underway (data not shown). Sequencing of the closely related P. mirabilis showed that Avr8 is also conserved in that species, but not in other clade 1c Phytophthora species.

[0146] Rpi-Smira2 is Localized on Chromosome IX

[0147] In previous studies, it was shown that the response to PITG_07558 correlates with field resistance that is conferred by the late blight resistance gene Rpi-Smira2 in cv. Sarpo Mira (Rietman et al. 2012). The fact that Rpi-Smira2 interacts with the same effector as R8 suggests that both genes localize on a similar position of chromosome IX. In order to prove this, the flanking markers to R8, 184-81 and CDP4 (Jo et al. 2011), were tested in Sarpo Mira.times.RH population (population SM*RH). In population SM*RH, flanking marker CDP4 located 1 cM distal to HR responses to AVR8 upon agroinfiltration (AVR8-HR) and the opposite flanking marker 184-81 2 cM proximal to AVR8-HR (FIG. 5). This position is similar to position of the R8 gene (Jo et al. 2011). For CDP3, a segregation of 22 presence to 4 absence in the tested genotypes was found which fitted a 5:1 ratio (X.sup.2=0.87, >0.05). Some CDP3 positive genotypes were not responsive to AVR8 while none of the AVR8 responsive genotypes lacked the marker band.

TABLE-US-00005 TABLE 5 Markers used for mapping of Rpi-Smira2 in the Fl population (Sarpo Mira x SEQ Tm Product Marker Primer Sequence (5'.fwdarw.3') ID NO Marker type (.degree. C.) size (bp) References CDP3 Hero4064F: 5 CDP/HaeIII 55 500 bp Jo et al. (2011) RRAGATTCAGCCATKGARATTAAGAAA 184-81 184-81F: CCACCGTATGCTCCGCCGTC 11 CAP SIRsaI 58 480 bp Jo et al. (2011) 184-81R: GTTCCACTTAGCCTTGTCTTGCTCA 12 CDP4 Sw55F: AGTCTCCAAACATTCCTGCTTCTC 6 CDP/MseI 55 277 bp Jo et al. (2011) Stm1021 Stm1021F: GGAGTCAAAGTTTGCTCACATC 7 SSR 58 210 bp Collins et al. (1999) Stm1021R: CACCCTCAACCCCCATATC 8 Sequences of adapter and adapter primer for CDP profiling MseI adapter Mse-ad-top: CCCGAAAGTATAGATCCCAT 13 Mse-ad-bottom: TAATGGGATCTATACTT 14 Blunt adapter B-ad-top: 15 van der Linden et al. (2004) ACTCGATTCTCAACCCGAAAGTATAGATCCCA B-ad-bottom: TGGGATCTATACTT 16 Adapter primer ACTCGATTCTCAACCCGAAAG 17

[0148] This suggested that CDP3 was present in duplex and that only one marker allele was linked to Rpi-Smira2 which was present in simplex. Therefore, we concluded that Rpi-Smira2 resides in the R8 locus on chromosome IX.

[0149] Functional Profiling of Wild Solanum Species for Response to AVR8

[0150] To determine the representation of R8 functional homologs in wild Solanum species, 98 genotypes (72 accessions of 40 species) which are geographically and phylogenetically diverse were selected from wild Solanum section Petota germplasm (Vleeshouwers et al. 2011b). Wild Solanum genotypes that display resistance to P. infestans isolate IPO-C in detached leaf tests as well as in field trials were selected. The plants were functionally tested for cell death responses to AVR8 by agroinfiltration. Also, the potato differential set MaR1 to MaR11 were included in this study. From these 109 genotypes, 60 genotypes showed nonspecific cell death responses to Agrobacterium or did not produce a response to the positive control construct and therefore could not be accurately grouped as responsive or nonresponsive. 35 genotypes that were well amenable to agroinfiltration did not show an Avr8 response. Twelve genotypes from various wild Solanum accessions and two genotypes from the potato differential set displayed specific cell death in response to AVR8 (Table 6). These include genotypes from S. demissum, S. tarijense, S. microdontum gigantophyllum, S. stoloniferum, S. schenkii and two unclassified Solanum section Petota species. From the potato differential set MaR1 to MaR11, as expected, MaR8 as well as MaR9 showed the response to AVR8 (Table 6). The presence of R8 in MaR9 confirms the conclusion of a previous study (Kim et al. 2012). We further tested all AVR8 responding genotypes using R8 flanking markers, and found that S. microdontum spp. gigantophyllum genotypes GIG712-6 and GIG715-4 had distinctive marker patterns, which lack the target bands that linked to the R8 resistance (data not shown). This suggests that AVR8 recognition specificity of these plants may be conferred by R gene(s) different from R8.

TABLE-US-00006 TABLE 6 AVR8 response in a selection of Solanum germplasm material and the Mastenbroek late blight differential set Country R3a/ Resistance Species Genotype.sup.a Series.sup.b of origin Avr8 pK7WG2 Avr3a (IPO-C field).sup.c S. species spp114-S.sup.f Demissa BOL + - + R S. demissum DMS345-1 Demissa MEX + - + R S. demissum DMS343-1 Demissa MEX + - + R S. demissum DMS344-18 Demissa GTM + - + R S. demissum DMS585-7 Demissa MEX + - + R S. demissum DMS585-1 Demissa MEX + - + R S. microdontum GIG712-6 Tuberosa BOL + - + R gigantophyllum S. microdontum GIG712-4 Tuberosa ARG + - + M gigantophyllum S. schenckii SNX213-1 Demissa MEX + - + R S. species spp891-1.sup.g Tuberosa BOL + - + R S. tarijense TAR852-5 Yungasensa BOL + - + M S. stoloniferum STO389-4 Longipedicellata MEX + - + R (S. demissum).sup.d MaR1 (MEX).sup.e - - + S (S. demissum).sup.d MaR2 (MEX).sup.e - - + S (S. demissum).sup.d MaR3 (MEX).sup.e - - + S (S. demissum).sup.d MaR4 (MEX).sup.e - - + S (S. demissum).sup.d MaR5 (MEX).sup.e - - - M (S. demissum).sup.d MaR6 (MEX).sup.e - - + S (S. demissum).sup.d MaR7 (MEX).sup.e - - + S (S. demissum).sup.d MaR8 (MEX).sup.e + - + R (S. demissum).sup.d MaR9 (MEX).sup.e + - + R (S. demissum).sup.d MaR10 (MEX).sup.e - - + S (S. demissum).sup.d MaR11 (MEX).sup.e - - + S .sup.aThe three letter code represents the Solanum species (Simmonds 1962). The first number represents the CBSG number for the accession followed by a genotype number. MEX: Mexico, BOL: Bolivia, ARG: Argentina, GTM: Guatemala. .sup.bClassified according to Hawkes (1990). .sup.cR (highly resistant): >8.5, M (moderately resistant): 4.5-8.0, phenotype data for two years' field trials (Vleeshouwers et al. 2011b). .sup.d,eThe resistance of potato differential set is originating from S. demissum from Mexico. .sup.fUnclassified Solanum, previously named S. astleyi. .sup.gUnclassified Response to Avr8 occurs in Central and South American Solanum species.

[0151] An AFLP-based phylogenetic tree of the Solanum species that were tested for response to AVR8 was created using the SolRgene database (FIG. 6; Jacobs et al. 2011; Vleeshouwers et al. 2011b). Basically, the tested wild genotypes were arranged into five different groups (FIG. 6). Group I and IV included genotypes from South America while groups II and V are from Central America. Genotypes from both Central and South America belong to group III. The AVR8 responding genotypes belonged to groups IV and V, which consist of genotypes derived from Mexico (Central America) and from Bolivia and Argentina (South America) (FIG. 6, Table 6). All five S. demissum genotypes were responsive to AVR8. The two unclassified genotypes that also respond to AVR8 were classified with S. demissum (based on AFLP patterns, Jacobs 2008). From the Mexican polyploid species S. stoloniferum and S. schenckii, additional responsive genotypes were identified. From South American origin, genotypes of S. tarijense and S. microdontum spp. gigantophyllum were found to respond to AVR8 (FIG. 6). In summary, we detected response to AVR8 in various Solanum species that originate from both the Central and the South American center of diversity of Solanum section Petota. Solanum, previously named S. ugentii.

Example 3: Cloning and Sequencing of the R8 Gene

[0152] Materials and Methods:

[0153] Plant Material

[0154] The potato differential plant MaR8, corresponding to plant 2424a(5) described by Black et al. (1953), was used for bacterial artificial chromosome (BAC) library construction. MaR8*Concurrent population (R8*C) was used for genetic mapping. These plant materials and cv Desiree, which was used for transformation, were maintained in vitro at Wageningen UR Plant Breeding. Nicotiana benthamiana was maintained as seed stock.

[0155] Bacterial Artificial Chromosome Library Construction and Screening

[0156] A first BAC library was produced by mechanical shearing of MaR8 genomic DNA and ligation of high molecular weight fragments into pCC1 at RxBiosciences (Rockville, USA). This first BAC library consisted of 768 simple pools of 200 individual BAC clones. Simple pools were stored at -80.degree. C. The average insert size was 55 kb, resulting in a 2.5* coverage of a haploid genome. A second BAC library was produced by Bio S&T (Montreal, Canada). MaR8 genomic DNA was fragmented by partial digestion with HindIII. Size selected fragments were cloned into pIndigoBAC-5. The average insert size was 100 kb (FIG. S1) and 750 simple pools of 400 individual BACs, representing a 10* coverage of the haploid genome, were stored at -80.degree. C. Markers described in Table S2 were used to screen the BAC libraries. Bacterial suspensions of positive pools were diluted and plated on LB agar plates containing chloramphenicol (12.5 .mu.g ml.sup.-1). After determining the bacterial titer of a positive pool, 2.times.96 subpools containing 50 individuals each were grown for 8 hours in deepwell blocks. After culture, PCR positive sub pools were plated on LB plates containing chloramphenicol and individual colonies were picked into 96 flat bottom microtitre plates. Positive BAC clones were subsequently identified by a third round of PCR screening.

[0157] DNA Sequencing and Bioinformatics Analysis

[0158] BAC clone sequencing was carried out using a shotgun strategy. Fragmentation, library production, 454 sequencing and contig assembly was performed at Macrogen (Seoul, Korea). BAC n2A2 was sequenced using PACBIO (GATC, Germany). Gene structures were predicted using FGENESH2.6 (Softberry) and protein sequences were deduced by translation of ORF using the standard genetic code. Multiple sequence alignments and phylogenetic analyses were conducted using CLUSTALX 1.81 (Thompson et al. 1997) available in the MegAlign Lasergene 9.0 software package (DNASTAR Inc., USA).

[0159] Subcloning of Candidate Genes

[0160] Primers were designed for subcloning RGA0.20-3.2 (FIG. 7) using Primer Select from the Lasergene 9.0 software package (DNASTAR Inc., USA) and were extended at the 5' end with recognition sites for eight cutter restriction enzymes (Table S3). Long-range PCR reactions were performed using Phusion.RTM. High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, USA) in a simple PCR program (98.degree. C. for 30 s followed by 24 cycles of 98.degree. C. for 10 s, 62-65.degree. C. for 30 s, 72.degree. C. for 5.5 min and a final extension time of 15 min at 72.degree. C.), and using BAC clones 3E3, 6A5, or n2E2 as templates. The resulting PCR products were subjected to G50 Sephadex purification using Illustra MicroSpin columns (GE Healthcare) followed by ligation to the PCR-BluntII-Topo vector using the Zero Blunt Topo PCR Cloning Kit (Invitrogen). Ligation products were transformed to ElectroMAX E. coli DH10B competent cells (Life technologies, Paisley, UK). The inserts of PCR positive colonies were sequenced using a primer walking strategy (700 bp by 700 bp) to confirm that no mutations were introduced. The purified PCR-BluntII-Topo clones were digested with AscI and SbfI, or with XmaI and SbfI which were present in the 5' extensions of the primer (Table 7), Sticky ends were subsequently dephosphorylated using TSAP (Pomega) and all enzymes were heat inactivated. The digestion mix was ligated to the AscI and SbfI or XmaI and SbfI sites of the binary vector pBINPLUS-PASSA or pBINPLUS-ASSAP (Jo et al. 2015), which are modified versions of pBINPLUS containing eight cutter multiple cloning sites (PASSA: P=PacI; A=AbsI; S=SrfI; S=SbfI; A=AscI, or the inversed orientation for pBINPLUS-ASSAP). Recombinant clones were selected that contained R8 between the SrfI and SbfI sites (pBINPLUS-PAS:R8:SA) or between the SbfI and AscI sites (pBINPLUS-PASS:R8:A or pBINPLUS-A:R8:SSAP).

TABLE-US-00007 TABLE 7 Primer used for RGA amplification and subcloning SEQ Restriction Tm ID Target Orientation site (.degree. C.) Sequence (5'...3') NO RGA0.20 F SrfI 63 catgcccgggcAAAACTTTCACGCACCCATAGGA 18 R SbfI gcacctgcaggAACAAGAGATGAATTAAGTCGGTAGC 19 RGA0.21 F SrfI 65 catgcccgggcTTTGTATTATGATTGGCCCTGTTCTGA 20 R SbfI gcacctgcaggCTGCGAGTATTGGTGGCTGACAT 21 RGA1.0 F SrfI 65 catgcccgggcCAACAATGCGGCGCTTTAGGA 22 R SbfI gcacctgcaggTGGTGCACTTGCCTGGACTTTA 23 RGA1.1 F Srfl 62 catgcccgggcCCTCCATTTTCCATTAAGTCTTGC 24 R SbfI gcacctgcaggAATGGTTTCATCAATGAATCTTTC 25 RGA1.2 F AscI 65 catggcgcgccTATTAAACAGGAAACACAAAAGCAGT 26 CA R XmaI catgcccgggcTTCCTTGCAAAAATCTCACTCACTATG 27 RGA3.1 F SrfI 62 catgcccgggcCACCTAACTGATTTGCTTC 28 R SbfI gcacctgcaggTCAAACTAAGACACTTAAATTA 29 RGA3.2 F SbfI 65 gcacctgcaggTCTTGTGGTTGTCTTGGTAGCAGGAG 30 R XmaI catgcccgggcGTAGAGAAAAAGGAGAAAGCACAGA 31 Rpi-smira2 F 63 CTGGATTTCTTCAAGATTCGTCGT 32 CDS R TTCAATCTCTTCGACTTCTTCTTACG 33 Rpi-smira2 F 63 AAAACTTTCACGCACCCATAGGA 34 5' end R AGTAAACTTTGACACCTTTAGTTCACCAT 35

[0161] Agrobacterium-Mediated Transient Co-Expression in N. benthamiana

[0162] Binary plasmids harbouring RGAs or Avr8 (Jo 2013) were transformed to A. tumefaciens strain AGL1 with an additional plasmid borne copy of VirG (van der Fits et al. 2000). Two leaves per plant and three replicates of 4 weeks old N. benthamiana seedlings were agroinfiltrated. A mixture of R3b and Avr3b (Li et al. 2011) were used as the positive control and empty pBINPLUS was used as a negative control. A. tumefaciens strains from frozen glycerol stocks were grown overnight at 28.degree. C. in 3 ml of LB medium supplemented with appropriate antibiotics. The next day these cultures were used to inoculate 15 ml of YEB medium (5 g beef extract, 5 g bacteriological peptone, 5 g sucrose, 1 g yeast extract, 2 ml 1 M MgSO4 in 1 liter of milliQ water) supplemented with antibiotics, 10 .mu.l/l of 200 mM acetosyringone and 1000 .mu.l/l of 1 M MES. On the third day, the cells were harvested and resuspended to a final OD.sub.600 of 0.2 in MMA (20 g sucrose, 5 g MS salts and 1.95 g MES in 1 liter of distilled water, adjusted to pH5.6 with KOH) supplemented with 1 ml/l of 200 mM acetosyringone in DMSO. Responses were scored 3 to 4 days after infiltration.

[0163] Transformation of Potato

[0164] Binary plasmids harbouring RGAs were transferred to A. tumefaciens strain AGL1 containing the helper plasmid pVirG (van der Fits et al. 2000). The stability of these clones in Agrobacterium was tested and overnight cultures of the transformed A. tumefaciens strain were used to transform susceptible cultivar Desiree (Heeres et al. 2002). The kanamycin resistant regenerants (transgenic events) were analysed by PCR to determine the presence of the desired R8 gene. Two replicates per transgenic event were transferred to the greenhouse for successive climate chamber assays or for planting in the field, respectively.

[0165] Late Blight Resistance Tests

[0166] P. infestans isolates and their corresponding (a)-virulence spectra that were used in this study are listed in Table 3. The late blight epidemic in Wageningen in the 2014 and 2015 field trials was a result of natural P. infestans infection that started at the end of June (2014) or half July (2015). Field trials in Wageningen in the years 2012 and 2013, in which recombinant plants from the extended 3020 population were tested, were inoculated at July 1.sup.st with IPO-C as described earlier (Jo et al. 2011). Field trials contained four replicates per genotype and late blight was assessed at weekly intervals after inoculation or after the first late blight symptoms were visible in susceptible control plants by observing late blight lesions, in a qualitative way. Resistant plants remained unaffected until the end of the experiment mid August, while susceptible plants had died already mid July.

[0167] Climate cell whole plant assays: Two replicates of transgenic Desiree or recombinant seedlings from population R8*C were propagated in vitro. The plants were transferred to potting soil and grown in the greenhouse at 22.degree. C. with a 10 h day/14 h night photoperiod and a relative humidity of 70-80%. One month after potting of the plants, they were transferred into a growth chamber and inoculated. Inoculum was prepared by washing of the oospores from two weeks old rye sucrose agar plates containing the isolates of interest. Plants were inoculated by placing four 141 droplets of inoculum (5.times.10.sup.4 zoospores/ml) on the adaxial side of at least three leaves per plant. Inoculated plants were kept for seven days in a climate chamber at 15.degree. C. and 100% humidity with a photoperiod of 16 h/8 h day/night. Late blight levels could be classified in three groups, resistant (no symptoms, limited hypersensitive response (HR)); intermediate resistance (large HR lesions or spreading HR lesions without sporulation) or susceptible (sporulating lesions). Genotypes classified in the resistant group or intermediate resistant group in climate chamber assays did not show significant late blight lesions until the end of the field trial experiments (resistant) and could easily be distinguished from the susceptible group.

[0168] Results

[0169] R8 Fine Mapping

[0170] In order to fine map R8, molecular markers were required to perform a recombinant screen in F1 population R8*C. R8 is located at the bottom end of chromosome 9, flanked by Tm-2.sup.2-like CDP markers at the centromer proximal side and by Sw-5 CDP markers on the distal side (Jo et al. 2011). The CDP markers were not suitable for high throughput recombinant screens and simple PCR markers needed to be developed. On the proximal side marker 184_81 had been described before, but a marker on the distal side of R8 remained to be developed. Screening of the tomato marker database revealed marker C2_At5g06360, which is located near the telomer of Chr9. MaR8 and cv Concurrent derived amplicons of this marker were screened for cleaved amplified polymorphisms, linked to resistance and this resulted in marker At5g06360_2-FspBI. Population R8*C was expanded to 1720 individuals and recombinants between markers At5g06360_2-FspBI and 184_81-RsaI (FIG. 7) were screened for. In total 36 recombinants were found and their resistance phenotype was determined in a whole plant late blight assay in a climate chamber. Marker CDP.sup.Hero3, which was identified previously, was still fully linked to resistance in this expanded population. Two recombinants were found between At5g06360_2-FspBI and CDP.sup.Hero3, while 34 recombinants were found between 184_81 and CDP.sup.Hero3 (FIG. 1).

[0171] BAC Landing and BAC Walking

[0172] A first BAC library was constructed from the genomic DNA of MaR8 plants. The library was screened using marker CDP.sup.Hero3 and BAC clones 1A6, 3E3 and 6G9 were identified. The insert of 3E3 was sequenced and revealed the presence of four complete (RGA1.1, 1.2, 3.1 and 3.2) and one truncated R gene analog (RGA3.3). The newly obtained sequences were used for new marker development. A screen for markers in the intergenic regions successfully identified two polymorphic markers named 3E3_5-HRM and 3E3_10-SCAR. Mapping of the new markers revealed that the right end of BAC 3E3 fell outside the mapping interval, excluding RGA3.3 as a R8 candidate (FIG. 7). No recombinants were found at the left end of the BAC indicating that RGAs 1.0, 1.1, 3.1, 3.2 and, so far unidentified additional RGAs could be R8 candidates. In order to close the genetic window, marker 3E3_10-SCAR was used for screening the BAC library and resulted in the isolation of BAC clone 6A5 (FIG. 7). Sequence analysis revealed one additional complete RGA (RGA1.0). A marker developed on the 6A5 BAC end (6A5F_3-HpyCH4IV) still did not show any recombinants with R8 resistance, so the genetic window was not closed yet. Marker 6A5F_3-HpyCH4IV was used to screen the BAC library but unfortunately no new positive BACs were identified. A new BAC library was generated using a different genome fragmentation method (partial restriction enzyme digestion instead of mechanical shearing that was used in constructing the first BAC library). Screening of the new library identified BAC clone n2E2. Sequence analysis revealed the presence of four additional complete RGAs (0.10, 0.11, 0.20, and 0.21.). A screen for markers in the intergenic regions revealed marker 2E2_1-hin1II. One recombinant was found between this marker and the late blight resistance and it was concluded that the genetic interval was now closed. A genomic region of 174 kb (FIG. 8) containing a cluster of 10 paralogous sequences was found and all sequences had high homology to Sw-5, a R gene from tomato that provides resistance to tomato spotted wilt virus (Brommonschenkel et al. 2000).

[0173] R8 Candidate Cloning and Complementation Analysis

[0174] The seven RGAs in the genetic window were subcloned in the binary vector pBINPLUS-PASSA for Agrobacterium mediated transformation of plants. Stable transgenic plants of the susceptible potato variety Desiree were produced and 10 to 47 events per construct were selected. Six out of seven constructs produced only transformation events that were susceptible to P. infestans isolate IPO-C (Table 8). Eight out of 47 events transformed with RGA0.20 were susceptible, while 39 events showed intermediate to strong late blight resistance in a whole plant assay in climate chambers (FIG. 9A). PCR analysis revealed that the 8 susceptible events contained only partial inserts of the T-DNA. RGA0.20 was therefore denoted as a strong R8 candidate. This idea was confirmed when the RGAs were co-expressed with Avr8 in the Nicotiana benthamiana. Only RGA0.20 induced a hypersensitive response (HR) when co-infiltrated with Avr8. The observed HR was a result of specific recognition since co-infiltration of Avr3b with RGA0.20 did not result in a HR (FIG. 9B). From these results we conclude that RGA0.20 is R8.

TABLE-US-00008 TABLE 8 Complementation analysis of R8 resistance in Desiree Climate Avr8 chamber whole response in Tested plant assay# Field trial# construct N. benthamiana events# S IR R S R RGA3.2 - 12 12 0 0 nd nd RGA3.1 - 10 10 0 0 nd nd RGA1.2 - 10 10 0 0 nd nd RGA1.1 - 10 10 0 0 nd nd RGA1.0 - 10 10 0 0 nd nd RGA0.21 - 15 15 0 0 nd nd RGA0.20 + 47 8 14 25 8 39 empty - 12 12 0 0 12 0 nd: not determined; #: number of events

[0175] R8 Sequence Annotation

[0176] The binary vector containing R8 that was used for complementation studies had an insert of 7011 bp. A 1680 bp 5' untranslated region, encompassing a functional promotor, is followed by a single open reading frame of 3738 bp, representing the R8 coding sequence, which is followed by a 1594 bp 3' untranslated region which encompasses a functional transcriptional terminator (FIG. 10). The encoded R8 protein shows a tripartite domain structure CC NB-ARC LRR, which is typical for intracellular plant disease resistance proteins. Conserved protein sub-domains can be distinguished in the central NB-ARC region and a set of 13 C-terminal leucine rich repeats are found (FIG. 11). When the R8 protein sequence was aligned with known R proteins from Solanaceae and phylogenetic analysis was performed, it was found that R8 constitutes a distinct clade with the tomato Sw-5 protein (Brommonschenkel, 2000), which provides resistance to tomato spotted wilt virus (FIG. 12). Sw-5 and R8 shared 88.6% homology, while homology to other R proteins that were aligned was 26.1%, in the case of Rpi-blb2, or less (Table 9).

TABLE-US-00009 TABLE 9 Percentages of homology between R proteins from Solanaceae. R8 R1 Sw-5 Rpi-blb2 Prf1 Rpi-chc1 Rpi-tar1 Rpi-vnt1 Rpi-sto1 Bs2 Bs4 gro1.4 26 27.1 25 23.7 28.4 21.8 22.1 24.4 22.1 28.6 14.9 16.2 *** 22.8 88.6 26.1 23.1 19.9 20.3 24.1 20.8 25.7 15.1 15 *** 22.6 23.6 27.2 19.9 19.5 24.5 20.4 26.6 13.9 14.4 *** 26 22.9 19.5 19.9 24.5 19.5 25.4 15 14.6 *** 22.5 19.3 19.3 21.4 21.1 25 16 16.4 *** 21.6 21.6 22.7 18.7 27.1 14.8 15.5 *** 98.2 19.9 34.7 21.7 15.6 15.5 *** 19.9 34.6 21.9 15.2 15.6 *** 23.3 23.2 14.4 14.9 *** 20.7 16.2 16.1 *** 16.1 15.9 *** 40.3 *** R2 R3a R3b Rpi-abpt Tm2{circumflex over ( )}2 R9a mcq1 N 26.1 20.7 20.3 26.4 25.4 26.3 24.9 15.8 NRC1 24.6 21.1 19.7 24.3 24.3 25.9 24.6 15.4 R8 25.3 20.7 19.7 25 25.5 26.3 24.8 14.4 R1 23.3 20.8 19.2 23.2 24.9 25.9 24.6 15 Sw-5 22.9 21.8 20.2 22.7 21.7 22.1 21.5 16.2 Rpi-blb2 23.9 18.7 18.3 23.3 23.6 24.3 22.7 14.3 Prf1 23.9 29.2 28.7 23.9 21.1 21.6 20.1 15.1 Rpi-chc1 24 28.8 28.3 24 20.9 21.7 20.1 14.9 Rpi-tar1 29.9 20.9 20.4 29.1 76.6 78.7 82 14.8 Rpi-vnt1 26 30.8 30.7 26.3 22.2 23.6 22.7 15 Rpi-sto1 23.8 21.3 19.3 23.6 23.7 23.6 23.1 15.3 Bs2 15.6 15.8 14.9 15.4 15.4 15.1 15.4 61.1 BS4 15.2 17.1 16.3 14.7 14.7 15 15.3 39.6 gro1.4 *** 24.4 24.2 95.5 28.9 28.9 28.8 15 R2 *** 72.4 24.2 20.5 22 20.6 16.1 R3a *** 24 19.9 20.7 20.3 15.2 R3b *** 28.3 27.9 28.2 14.9 Rpi-abpt *** 74 79 15.3 Tm2{circumflex over ( )}2 *** 81.7 14.9 R9a *** 15.4 Rpi-mcq1 *** N

Example 4: Late Blight Challenges

[0177] Materials and Methods

[0178] Construction of Vectors Harbouring Combinations of R8 with Other Late Blight Resistance Genes

[0179] Combinations of R8 with other late blight R genes were made by inserting Rpi-sto1, Rpi-blb3, Rpi-edn2, Rpi-vnt1.1, or Rpi-blb2 into the SbfI and AscI or SrfI and SbfI sites of the recipient vectors containing R8 (pBINPLUS-PAS:R8:SA, pBINPLUS-PASS:R8:A or pBINPLUS-A:R8:SSAP). This resulted in pBINPLUS-PAS:R8:S:Rpi-sto1:A, pBINPLUS-A:R8:S:Rpi-blb3:SAP, pBINPLUS-PAS:R8:S:Rpi-edn2:A, pBINPLUS-PA:Rpi-vnt1.1:SS:R8:A, pBINPLUS-PAS:Rpi-blb2:S:R8:A.

[0180] Late Blight Resistance Tests.

[0181] P. infestans isolates and their corresponding (a)-virulence spectra that were used in this study are listed in Table 9. The late blight epidemic in Wageningen, the Netherlands, in the 2014 and 2015 field trial was a result of natural P. infestans infection that started at the end of June. Field trials contained four replicates per genotype and late blight was assessed by observing late blight lesions after inoculation or after the first late blight symptoms were visible in susceptible control plants (2014), in a qualitative way. Resistant plants remained unaffected until the end of the experiment mid August, while susceptible plants had died already mid July.

[0182] Climate cell whole plant assays: Two replicates of transgenic Desiree or recombinant seedlings from population 3020 were propagated in vitro. The plants were transferred to potting soil and grown in the greenhouse at 22.degree. C. with a 10 h day/14 h night photoperiod and a relative humidity of 70-80%. One month after potting of the plants, they were transferred into a growth chamber and inoculated. Inoculum was prepared by washing of the oospores from two weeks old rye sucrose agar plates containing the isolates of interest. Plants were inoculated by placing four 10 .mu.l droplets of inoculum (5.times.104 zoospores/ml) on the adaxial side of at least three leaves per plant. Inoculated plants were kept for seven days in a climate chamber at 15.degree. C. and 100% humidity with a photoperiod of 16 h day/8 h night. Late blight levels could be classified in three groups, resistant (no symptoms, limited hypersensitive response (HR)); intermediate resistance (large HR lesions or spreading HR lesions without sporulation) or susceptible (sporulating lesions). Genotypes classified in the resistant group or intermediate resistant group in climate chamber assays did not show significant late blight lesions until the end of the field trial experiments (resistant) and could easily be distinguished from the susceptible group.

TABLE-US-00010 TABLE 10 Virulence spectra of different P. infestans isolates used in this study. Isolate Origin A-virulence spectrum Reference NL09066 The Netherlands avrsto1, Avrblb3, Avr8, G. Kessel Avredn2, Avrblb2, Avrvnt1 IPO-C The Netherlands Avrsto1, avrblb3, Avr8, (Zhu et Avredn2, Avrblb2, Avrvnt1 al. 2014) US-22 USA (US090017) Avrsto1, Avrblb3, avr8, G. Kessel Avredn2, Avrblb2, Avrvnt1 NL12097 The Netherlands Avrsto1, Avrblb3, Avr8, G. Kessel avredn2, Avrblb2, Avrvnt1 NL12003 The Netherlands Avrsto1, Avrblb3, Avr8, G. Kessel Avredn2, avrblb2, Avrvnt1 Ec-1 Ecuador Avrsto1, Avrblb3, Avr8, (Pel 2010) Avredn2, Avrblb2, avrvnt1

[0183] Transformation of Potato

[0184] Binary plasmids harbouring combinations of late blight R genes were transferred to A. tumefaciens strain AGL1 containing the helper plasmid pVirG (Van der Fits et al. 2000). The stability of these clones in Agrobacterium was tested and overnight cultures of the transformed A. tumefaciens strain were used to transform susceptible cultivar Desiree (Heeres et al. 2002). The kanamycin resistant regenerants (transgenic events) were analysed by PCR to determine the presence of the R8 and the second late blight resistance gene. Two or four plants per transgenic event were transferred to the greenhouse for climate chamber assays or for planting in the field, respectively.

[0185] Results:

[0186] Potential of R8 for protection against late blight.

[0187] Not only the Avr8 recognition was maintained by the cloned R8 gene (Table 8), but also the broad resistance spectrum of R8 against the current P. infestans population was maintained. 39 transgenic events provided excellent late blight resistance to natural late blight infection in 2014 and 2015 in field trials in Wageningen, The Netherlands (Table 1, FIG. 3).

[0188] It was described previously that R8 is the same or a similar gene as Rpi-smira2, the major constituent of the durably late blight resistant variety Sarpo Mira (Jo 2013). To confirm this suggestion, we set out to clone the Rpi-smira2 gene using R8 specific primers (Table S3) that amplified the coding sequence (CDS) or 1690 nucleotides of the 5' untranslated region (UTR) and the first 745 bp of the CDS. Three independent Rpi-smira2 clones of each amplicon were sequenced and the consensus showed 100% identity with R8 in the CDS. At position -723 in the 5'UTR there was one T>C polymorphism in R8 vs Rpi-smira2. This clearly confirmed that Rpi-smira2 is the same gene as R8.

[0189] Stacking of R8 with Other Late Blight R Genes to Achieve Durable Resistance

[0190] The durability of Sarpo mira's resistance was associated with the stacking of multiple R genes (Rietman et al. 2012). To pursue similar or even better durability, we set out to produce stacks of R8 with other late blight R genes in order to create material for future durability challenges. Combinations of R8 with Rpi-sto1, Rpi-blb3, Rpi-edn2, Rpi-vnt1.1 and Rpi-blb2 were made in binary vectors and these constructs were transformed to potato variety Desiree. From each construct 21 transformation events were harvested and tested for the presence and functional expression of both R genes. For this purpose, the events were inoculated with differential P. infestans isolates (Table 10) that had overcome one of the R genes in the stack. There was a remarkable difference in the frequency by which events were produced that were resistant to both differential isolates (Table 3). The most efficient combinations were R8:Rpi-edn2,

[0191] Rpi-vnt1.1:R8 and Rpi-blb2:R8. In 2015 these plants were exposed to durability challenges in field trials in 2015. Indeed, the plants that functionally expressed both R8 and the second R gene remained devoid of any late blight symptoms (Table 11).

TABLE-US-00011 TABLE 11 Combinations of R8 with other late blight R genes in one binary vector transformed to Desiree. Frequency of Immune** Frequency that both R plants among events with Insert in binary genes are active in both R genes active in the vector transgenic events* field R8:Rpi-sto1 0.00 -- Rpi-blb3:R8 0.08 -- R8:Rpi-edn2 0.55 1.0 Rpi-vnt1.1:R8 0.86 1.0 Rpi-blb2:R8 0.95 1.0 *21 events per construct were tested for the activity of both R genes using differential isolates. **immunity is defined as absence of symptoms until the end of the growing season of 2015

Sequence CWU 1

1

40125DNAArtificial Sequenceprimermisc_feature(11)..(11)Imisc_feature(22)..(22)I 1yytkrthgtm ntkgatgayr tntgg 25223DNAArtificial Sequenceprimermisc_feature(3)..(3)Imisc_feature(15)..(15)Imisc_feature(21- )..(21)I 2gcnarwgtwg tyttnccyra ncc 23321DNAArtificial Sequenceprimer 3gtaacaagtc atgtatgcga c 21422DNAArtificial Sequenceprimer 4gccaaatagt attgtcaagc tc 22527DNAArtificial Sequenceprimer 5rragattcag ccatkgarat taagaaa 27624DNAArtificial Sequenceprimer 6agtctccaaa cattcctgct tctc 24722DNAArtificial Sequenceprimer 7ggagtcaaag tttgctcaca tc 22819DNAArtificial Sequenceprimer 8caccctcaac ccccatatc 19920DNAArtificial Sequenceprimer 9tgaatggact ggtgatctgc 201021DNAArtificial Sequenceprimer 10ttggaaagaa ttggcttttg a 211120DNAArtificial Sequenceprimer 11ccaccgtatg ctccgccgtc 201225DNAArtificial Sequenceprimer 12gttccactta gccttgtctt gctca 251320DNAArtificial Sequenceprimer 13cccgaaagta tagatcccat 201417DNAArtificial Sequenceprimer 14taatgggatc tatactt 171532DNAArtificial Sequenceprimer 15actcgattct caacccgaaa gtatagatcc ca 321614DNAArtificial Sequenceprimer 16tgggatctat actt 141721DNAArtificial Sequenceprimer 17actcgattct caacccgaaa g 211834DNAArtificial Sequenceprimer 18catgcccggg caaaactttc acgcacccat agga 341937DNAArtificial Sequenceprimer 19gcacctgcag gaacaagaga tgaattaagt cggtagc 372038DNAArtificial Sequenceprimer 20catgcccggg ctttgtatta tgattggccc tgttctga 382134DNAArtificial Sequenceprimer 21gcacctgcag gctgcgagta ttggtggctg acat 342232DNAArtificial Sequenceprimer 22catgcccggg ccaacaatgc ggcgctttag ga 322333DNAArtificial Sequenceprimer 23gcacctgcag gtggtgcact tgcctggact tta 332435DNAArtificial Sequenceprimer 24catgcccggg ccctccattt tccattaagt cttgc 352535DNAArtificial Sequenceprimer 25gcacctgcag gaatggtttc atcaatgaat ctttc 352639DNAArtificial Sequenceprimer 26catggcgcgc ctattaaaca ggaaacacaa aagcagtca 392738DNAArtificial Sequenceprimer 27catgcccggg cttccttgca aaaatctcac tcactatg 382830DNAArtificial Sequenceprimer 28catgcccggg ccacctaact gatttgcttc 302933DNAArtificial Sequenceprimer 29gcacctgcag gtcaaactaa gacacttaaa tta 333037DNAArtificial Sequenceprimer 30gcacctgcag gtcttgtggt tgtcttggta gcaggag 373136DNAArtificial Sequenceprimer 31catgcccggg cgtagagaaa aaggagaaag cacaga 363224DNAArtificial Sequenceprimer 32ctggatttct tcaagattcg tcgt 243326DNAArtificial Sequenceprimer 33ttcaatctct tcgacttctt cttacg 263423DNAArtificial Sequenceprimer 34aaaactttca cgcacccata gga 233529DNAArtificial Sequenceprimer 35agtaaacttt gacaccttta gttcaccat 29367011DNASolanum tuberosumCDS(1680)..(5417) 36aaaactttca cgcacccata ggaagtgaaa aacacttatt ttgtcatatc agcattttgt 60atttaatagg taatttttta aacaatttaa gtgttcaata ggtaatagtc ctagttaaga 120tgtctaactg aaatatacaa ataactttta aggaatcgtg aatgacttaa gcctaattta 180attggtaaat atgtgttatc ccgaaagatg tatggatgaa ttatcaccac tacttttttt 240aatccctgtt tgagatctcg atgactattc ttagtctaat tgttctgaca ttatttggtc 300aataaagcat gtgaatacta gtctaataca tgaatttcaa tctattttaa agaaggagac 360agcattaatc ttacacctac cacctaaatc aacttgctga tacaacttgc ctttttctaa 420atattaaata atatctgact cataagttaa taatttatat atataaaaaa aggagtcatc 480attttaaatt tatagaaata ctcatattct atttgaatgg attgtccgtg ccaagtgaaa 540atattaactt aaagagtaat tatttgttac tattattatt gactagtgga tctttataaa 600tttatgtgca tttgaaagtt tgtaatcgtg aatatttatt tataaaagaa acatgaaaat 660atgtaattca tcaatgcgat gctttagaat attttgatat cttatttaca aatgatgatt 720tactcattcg gtaagattgc ttaagaatta gataactttt gatagttttc acaacttgag 780aattttcata tttatgagaa aaaataactt catgtccgac ccaatacccc ttaattatat 840gctaaacaaa tatcaaaata gttcatagtt taagtatgaa attagcaaat cggacataat 900tttgggggat tttcctaagg agtcgtttgg cgtgaggtat gaggtataaa aattttggaa 960taaaatatag gattatttta ttatgtattt gattggaggt actagatagt cctttgatta 1020tttatcccaa tatttatact ttagtgatgg aataagttat ttcatataca tgttaaataa 1080cttattgtga gataattact ctcaaaataa cttattctcg accaaacgac ccttaaaaaa 1140aggactaagt aatatagttg tattattgag gcactttaca gctaccacct aaagcaactt 1200gctactacaa ttcatctctt gttaggtata agaattatta gagagacaga agaactctct 1260tccttcaaac cttcaaactt ccattcattt tatttcaatg aatttttttc ttatatcacc 1320tattgcgtaa acttggaaat atatctttgt tggaatccaa atctgagaaa ttgaagattc 1380ttgtcaacta actgtaagtc atcttcttca tgaattactt ttttcccata gatacagaat 1440tagagagttg atttctcaaa tggttcactc aactattagt tagtatctca taaaattgaa 1500aatgtaagaa agacgatttt ctcagataat aattagttaa gtgacatttg acaatatgaa 1560aatggtacat aagatgtata acttttcaca agcattaaca tagcttcaaa cactagatat 1620tgaaagacat gaacctaatt ccatgagttg gtgaacttgc aggaattttt ctacaaaaa 1679atg aat gaa aat gaa att gag gaa atg tta gat cac cta aga agg atc 1727Met Asn Glu Asn Glu Ile Glu Glu Met Leu Asp His Leu Arg Arg Ile 1 5 10 15 aaa att gaa ggt aac ctg gat ttc ttc aag att cgt cgt att ggg gat 1775Lys Ile Glu Gly Asn Leu Asp Phe Phe Lys Ile Arg Arg Ile Gly Asp 20 25 30 ctt gat att gtg cta aga gtt ttt aga acc ttt ata aag tat cat gtt 1823Leu Asp Ile Val Leu Arg Val Phe Arg Thr Phe Ile Lys Tyr His Val 35 40 45 ctt tta cct gat tgt ttt gtc aaa ctc aca atg aat gcc gaa tgg act 1871Leu Leu Pro Asp Cys Phe Val Lys Leu Thr Met Asn Ala Glu Trp Thr 50 55 60 gtg gaa atg ctt cac cgg gta ttt gat ggg ata tca gat gaa tgt aaa 1919Val Glu Met Leu His Arg Val Phe Asp Gly Ile Ser Asp Glu Cys Lys 65 70 75 80 act aac ctt aat ttg gaa agg cta gaa tca cat ttg ttg gaa ttc ttt 1967Thr Asn Leu Asn Leu Glu Arg Leu Glu Ser His Leu Leu Glu Phe Phe 85 90 95 gaa ggt aac tcc agt tta agt tac aat tat gag ttg aat gat ttt gat 2015Glu Gly Asn Ser Ser Leu Ser Tyr Asn Tyr Glu Leu Asn Asp Phe Asp 100 105 110 ctg tcg aaa tat atg gat tgt ctg gaa aaa att cta aat gat gta cta 2063Leu Ser Lys Tyr Met Asp Cys Leu Glu Lys Ile Leu Asn Asp Val Leu 115 120 125 atg atg ttc ctg gaa aag ggt agg tcc tgt tat ccc ata gaa aaa ctt 2111Met Met Phe Leu Glu Lys Gly Arg Ser Cys Tyr Pro Ile Glu Lys Leu 130 135 140 gca ata cag cta tct ata aag aaa ctg aaa att gtt caa aag aaa atg 2159Ala Ile Gln Leu Ser Ile Lys Lys Leu Lys Ile Val Gln Lys Lys Met 145 150 155 160 ata ttt ttg aga tac ata tat acc aca gag ata aat ggt aac gtc aac 2207Ile Phe Leu Arg Tyr Ile Tyr Thr Thr Glu Ile Asn Gly Asn Val Asn 165 170 175 tat gag aag ctg gaa tgt ttg gag act cga att cag ttc att gct aac 2255Tyr Glu Lys Leu Glu Cys Leu Glu Thr Arg Ile Gln Phe Ile Ala Asn 180 185 190 act gtg gga caa ttt tgt ttg gcc gta tta gat tat gtt gct gat att 2303Thr Val Gly Gln Phe Cys Leu Ala Val Leu Asp Tyr Val Ala Asp Ile 195 200 205 gaa ttt agt gat aat aat gat atc ttt aat ata cct ccg tat cta tta 2351Glu Phe Ser Asp Asn Asn Asp Ile Phe Asn Ile Pro Pro Tyr Leu Leu 210 215 220 tca ttg att gtg ttt gtg gag ctg gaa atg aag aag att ttt cat ggt 2399Ser Leu Ile Val Phe Val Glu Leu Glu Met Lys Lys Ile Phe His Gly 225 230 235 240 gaa cta aag gtg tca aag ttt act caa tca aaa act ttc aag gac aag 2447Glu Leu Lys Val Ser Lys Phe Thr Gln Ser Lys Thr Phe Lys Asp Lys 245 250 255 aaa tta cca aaa gaa ttt tca gat ctt ctc caa tat ctg ttg atg tat 2495Lys Leu Pro Lys Glu Phe Ser Asp Leu Leu Gln Tyr Leu Leu Met Tyr 260 265 270 ctc aga aac gaa aaa ctc gag aac ttt cct aat aat atc tct gct caa 2543Leu Arg Asn Glu Lys Leu Glu Asn Phe Pro Asn Asn Ile Ser Ala Gln 275 280 285 aat att gat gtg gca ata gaa ttc ttg ttg gtt ttc ctt gat gct gat 2591Asn Ile Asp Val Ala Ile Glu Phe Leu Leu Val Phe Leu Asp Ala Asp 290 295 300 gtg tca aat cac gtt att aat ggt aac tgg ttg aat gag gtc ttg tta 2639Val Ser Asn His Val Ile Asn Gly Asn Trp Leu Asn Glu Val Leu Leu 305 310 315 320 aag gtt gga gct ata gcg ggt gat att cta tat gta att caa aag ctt 2687Lys Val Gly Ala Ile Ala Gly Asp Ile Leu Tyr Val Ile Gln Lys Leu 325 330 335 ctt cct aga tct ata aac aaa gat gac act agc aaa ata agt ttt tgc 2735Leu Pro Arg Ser Ile Asn Lys Asp Asp Thr Ser Lys Ile Ser Phe Cys 340 345 350 tcg ata cag ata ttg gag aag act aaa gat ctg aag gca caa gtt gag 2783Ser Ile Gln Ile Leu Glu Lys Thr Lys Asp Leu Lys Ala Gln Val Glu 355 360 365 acg tac tac aaa tcc tta aaa ttt act cca tct cag ttc ccc acc ttt 2831Thr Tyr Tyr Lys Ser Leu Lys Phe Thr Pro Ser Gln Phe Pro Thr Phe 370 375 380 ggt gga ttg agc ttt ctg gat tct ctt tta agg aaa ctg aat gag atg 2879Gly Gly Leu Ser Phe Leu Asp Ser Leu Leu Arg Lys Leu Asn Glu Met 385 390 395 400 tcg aaa tct aag tct ggt tta gat ttc ctg atg aaa cct ctt tta ggg 2927Ser Lys Ser Lys Ser Gly Leu Asp Phe Leu Met Lys Pro Leu Leu Gly 405 410 415 aat ttg gag aaa gag tta tca tct ctt aca tcc att tta gag aag gag 2975Asn Leu Glu Lys Glu Leu Ser Ser Leu Thr Ser Ile Leu Glu Lys Glu 420 425 430 cta tca tcc att ttc aga gat gtc gtg cac cac gaa cat aaa att cct 3023Leu Ser Ser Ile Phe Arg Asp Val Val His His Glu His Lys Ile Pro 435 440 445 aaa gat ctt cag aga cgt acc atc aat ttg gca tat gag gct gag gtt 3071Lys Asp Leu Gln Arg Arg Thr Ile Asn Leu Ala Tyr Glu Ala Glu Val 450 455 460 gcc att gac tct att ctt gct cag tat aat gct ttt ttg cat att ttt 3119Ala Ile Asp Ser Ile Leu Ala Gln Tyr Asn Ala Phe Leu His Ile Phe 465 470 475 480 tgc tca ctt cct aca att tta aaa gag atc aag caa att aat gca gag 3167Cys Ser Leu Pro Thr Ile Leu Lys Glu Ile Lys Gln Ile Asn Ala Glu 485 490 495 gtg act gag atg tgg tca gca aac att cct ctt aat cct cgc tac gtg 3215Val Thr Glu Met Trp Ser Ala Asn Ile Pro Leu Asn Pro Arg Tyr Val 500 505 510 gct gct cca ttt aaa cat ctg cca gct cga cat agc aat ctt gtg act 3263Ala Ala Pro Phe Lys His Leu Pro Ala Arg His Ser Asn Leu Val Thr 515 520 525 gat gag gag gta gtg ggt ttt gag aat aaa gca gaa aaa cta att ggt 3311Asp Glu Glu Val Val Gly Phe Glu Asn Lys Ala Glu Lys Leu Ile Gly 530 535 540 tat ctg att aga ggt aca aat gag cta gac gtc atc cca att gta ggc 3359Tyr Leu Ile Arg Gly Thr Asn Glu Leu Asp Val Ile Pro Ile Val Gly 545 550 555 560 atg ggg gga caa ggg aaa acg aca att gct aga aag ttg tac aat aat 3407Met Gly Gly Gln Gly Lys Thr Thr Ile Ala Arg Lys Leu Tyr Asn Asn 565 570 575 gac atc att gtt tct cgc ttt aat gtt cga gca tgg tgc atc att tct 3455Asp Ile Ile Val Ser Arg Phe Asn Val Arg Ala Trp Cys Ile Ile Ser 580 585 590 caa aca tat agc cga aga gag cta tta caa gag att ttc agt caa gtt 3503Gln Thr Tyr Ser Arg Arg Glu Leu Leu Gln Glu Ile Phe Ser Gln Val 595 600 605 acg ggc tcc aag gac aag gaa gat gag gta ggc aaa ctt gct gac agg 3551Thr Gly Ser Lys Asp Lys Glu Asp Glu Val Gly Lys Leu Ala Asp Arg 610 615 620 ttg agg aaa agc cta atg gga aag aga tat ctc att gta ttg gat gat 3599Leu Arg Lys Ser Leu Met Gly Lys Arg Tyr Leu Ile Val Leu Asp Asp 625 630 635 640 atg tgg gat tgt atg gta tgg gat gac tta agg ctt tct ttt cca gat 3647Met Trp Asp Cys Met Val Trp Asp Asp Leu Arg Leu Ser Phe Pro Asp 645 650 655 gat gga atc aga agt aga ata gtc gta aca act cga ctt gaa gaa gtg 3695Asp Gly Ile Arg Ser Arg Ile Val Val Thr Thr Arg Leu Glu Glu Val 660 665 670 ggt aag caa gtc aag aac cat act gat cct tat tct ctt cca ttc ctc 3743Gly Lys Gln Val Lys Asn His Thr Asp Pro Tyr Ser Leu Pro Phe Leu 675 680 685 aca aca aaa gag agt tgc caa ttg ctg cag aaa aaa gtg ttt caa aag 3791Thr Thr Lys Glu Ser Cys Gln Leu Leu Gln Lys Lys Val Phe Gln Lys 690 695 700 gaa gat tgc ccg cct gaa cta caa tat gtg agt caa gca gtt gca gaa 3839Glu Asp Cys Pro Pro Glu Leu Gln Tyr Val Ser Gln Ala Val Ala Glu 705 710 715 720 aaa tgc aaa gga ctg ccc cta gtg gtt gtc ttg gta gct gga ata atc 3887Lys Cys Lys Gly Leu Pro Leu Val Val Val Leu Val Ala Gly Ile Ile 725 730 735 aaa aaa agg aaa atg gaa gaa tct tgg tgg aat gag gtg aaa gat gct 3935Lys Lys Arg Lys Met Glu Glu Ser Trp Trp Asn Glu Val Lys Asp Ala 740 745 750 tta ttt gac tat ctt gac agt gag ttc gaa gaa tac agt cta gcg act 3983Leu Phe Asp Tyr Leu Asp Ser Glu Phe Glu Glu Tyr Ser Leu Ala Thr 755 760 765 atg cag ttg agt ttt gat aac cta gct gat tgt tta aag cct tgt ctt 4031Met Gln Leu Ser Phe Asp Asn Leu Ala Asp Cys Leu Lys Pro Cys Leu 770 775 780 ctt tat atg ggg atg ttt ctg gag gac gca aga att cca gtg tct aaa 4079Leu Tyr Met Gly Met Phe Leu Glu Asp Ala Arg Ile Pro Val Ser Lys 785 790 795 800 ttg ata agc tta tgg att gct gaa gga ttc gtg gag aac act gaa tct 4127Leu Ile Ser Leu Trp Ile Ala Glu Gly Phe Val Glu Asn Thr Glu Ser 805 810 815 ggg aga tta atg gaa gag gaa gct gaa ggt tac ttg atg gat ctc att 4175Gly Arg Leu Met Glu Glu Glu Ala Glu Gly Tyr Leu Met Asp Leu Ile 820 825 830 agc agt aac gtg gta att gtt tca aag aaa ggt tat aat ggt aaa gtc 4223Ser Ser Asn Val Val Ile Val Ser Lys Lys Gly Tyr Asn Gly Lys Val 835 840 845 aaa tgc tgc cag gtt cat gat gtt gtg cat cac ttt tgc ttg aag aag 4271Lys Cys Cys Gln Val His Asp Val Val His His Phe Cys Leu Lys Lys 850 855 860 agt aga gaa gaa aag ttt atg ctt gca gtg aag ggt caa tat atc cag 4319Ser Arg Glu Glu Lys Phe Met Leu Ala Val Lys Gly Gln Tyr Ile Gln 865 870 875 880 ttt caa ccg ttg gat tgg aag gga agt cga gtg agc ttc agt ttc agt 4367Phe Gln Pro Leu Asp Trp Lys Gly Ser Arg Val Ser Phe Ser Phe Ser

885 890 895 gaa gag ctt tcc aag ttt gca tct ctg gtt tcc aaa aca cag aag cct 4415Glu Glu Leu Ser Lys Phe Ala Ser Leu Val Ser Lys Thr Gln Lys Pro 900 905 910 ttc cac caa cac ttg agg tct ctg ata acg gct aat ggt gga gaa tct 4463Phe His Gln His Leu Arg Ser Leu Ile Thr Ala Asn Gly Gly Glu Ser 915 920 925 att gat gtg att ccc gtc tgt cag att aat gaa ttg cga ctt ctt aag 4511Ile Asp Val Ile Pro Val Cys Gln Ile Asn Glu Leu Arg Leu Leu Lys 930 935 940 gtc ttg gat ttg agt tct tat tat gtg gag tct ttg tgg tta gct aga 4559Val Leu Asp Leu Ser Ser Tyr Tyr Val Glu Ser Leu Trp Leu Ala Arg 945 950 955 960 tta aac cca ctt aat cag ctg aag tac ctc gca gtt tgg gca ggt act 4607Leu Asn Pro Leu Asn Gln Leu Lys Tyr Leu Ala Val Trp Ala Gly Thr 965 970 975 ttc tat ttt gat cca caa tca cat ctg ccc cat ata gaa act tta att 4655Phe Tyr Phe Asp Pro Gln Ser His Leu Pro His Ile Glu Thr Leu Ile 980 985 990 gtg acg agt tgt ttt tat ggt gta cgg tta cca gtg tct ttt tgg gaa 4703Val Thr Ser Cys Phe Tyr Gly Val Arg Leu Pro Val Ser Phe Trp Glu 995 1000 1005 atg gaa aaa tta agg cat gtt cat ttt gct ggc gct ggt ttt gct 4748Met Glu Lys Leu Arg His Val His Phe Ala Gly Ala Gly Phe Ala 1010 1015 1020 atg cag gga ctc ttt gaa gga tcc tct aaa ttg gaa aat ttg agg 4793Met Gln Gly Leu Phe Glu Gly Ser Ser Lys Leu Glu Asn Leu Arg 1025 1030 1035 ata tta aag aaa att gag gaa ttt cca att gat agg ctg gat gtg 4838Ile Leu Lys Lys Ile Glu Glu Phe Pro Ile Asp Arg Leu Asp Val 1040 1045 1050 tta tca agg agg tgt cct aat ctt caa caa ctt caa atc aca ttt 4883Leu Ser Arg Arg Cys Pro Asn Leu Gln Gln Leu Gln Ile Thr Phe 1055 1060 1065 gag gat gat gta gag cct ttt tgt ccc aaa ttg gag agt ctt acc 4928Glu Asp Asp Val Glu Pro Phe Cys Pro Lys Leu Glu Ser Leu Thr 1070 1075 1080 cag ctt caa gaa ctt caa ctt tcc ttt gtg cat ccc cgc att cta 4973Gln Leu Gln Glu Leu Gln Leu Ser Phe Val His Pro Arg Ile Leu 1085 1090 1095 tcc ggg tta cag ttg cct tca aat tta aac aaa ttg gta ctt aaa 5018Ser Gly Leu Gln Leu Pro Ser Asn Leu Asn Lys Leu Val Leu Lys 1100 1105 1110 gga att cat atg gaa agt gct att tcc ttc att gcg gaa cta cca 5063Gly Ile His Met Glu Ser Ala Ile Ser Phe Ile Ala Glu Leu Pro 1115 1120 1125 agc ctg gag tat ctc caa tta cta gat gtg tgt ttt cct caa tca 5108Ser Leu Glu Tyr Leu Gln Leu Leu Asp Val Cys Phe Pro Gln Ser 1130 1135 1140 gaa gag tgg tgc ctt gga gat atc acg ttc cat aaa ctt aag ttg 5153Glu Glu Trp Cys Leu Gly Asp Ile Thr Phe His Lys Leu Lys Leu 1145 1150 1155 ttg aaa ctg gtg cag tta aat atc tca aag tgg gat gcc tcg gag 5198Leu Lys Leu Val Gln Leu Asn Ile Ser Lys Trp Asp Ala Ser Glu 1160 1165 1170 gaa tca ttt ccc ttg ctt gaa aca ctt gtt ata aaa aag tgt gat 5243Glu Ser Phe Pro Leu Leu Glu Thr Leu Val Ile Lys Lys Cys Asp 1175 1180 1185 gac ctt gag gag atc cca ctt agc ttt gct gat att cca tca ttg 5288Asp Leu Glu Glu Ile Pro Leu Ser Phe Ala Asp Ile Pro Ser Leu 1190 1195 1200 aaa cag att aag ttg att ggg tct tgg aaa gta tct atg gag gct 5333Lys Gln Ile Lys Leu Ile Gly Ser Trp Lys Val Ser Met Glu Ala 1205 1210 1215 tca gct gtg aga att aag gaa gaa gtc gaa gag att gaa gga tgt 5378Ser Ala Val Arg Ile Lys Glu Glu Val Glu Glu Ile Glu Gly Cys 1220 1225 1230 gac cgt ata gac ctc gta aga aga agt cga aga gat tga aagatgtgac 5427Asp Arg Ile Asp Leu Val Arg Arg Ser Arg Arg Asp 1235 1240 1245 cgtatagacc ttgtcaaaga atactgaagt ctattttgtt gctagctcat tctgttattg 5487taacactagt agtgtttgct atgtttgttt tgattgatca actctttttt atgtatgata 5547tgattggaga caaatataca tcagatcgtc tttcactaat ttcgtgtttt tatgcagctt 5607tataattttg accaatttct ctccacttac atcttcatag ctcaatacac atcaaccatg 5667ttgttgttgt tgttgttgtt gagtacttct tgattttcta attcgatgaa catccgaaaa 5727attttaaggt taaactgctt cttgttgctg taggtgcaat gttgaaggat ctctttatta 5787tgtgttcagg tgcctctttt agaagttgaa attcatacag aatgaagcta ataaatttgt 5847tcttgtcatt catcgatcat ttgaactttg caacatatga acttaagata tagtaaaaat 5907atagtatgac attcgagtcc cctccgaata cagagtcgtc tatgttaggg agcgtttcac 5967ccccaatgtg ggactttatt acgtcgtgaa tccattgtat cgggctccaa tgtgggtacc 6027ggacattggg tggaaacaaa aaaatgtata tagtacgaca tgaaatgaat aaataagggc 6087atttgaccct taagaaccaa aaaaatcaat cttttgtatt atgattggcc ctgttctgag 6147aacagactcc attcacaaga tctatggaag aattatcacc actccttttt tatccttgtt 6207tgatttatga catatgattt atcctttttt gaattatgtt tttttaaaaa aatataaaaa 6267cttattcata agtttatatt ttgtaaaaaa gaacaaatca ttattttcag ttttacaaaa 6327ataaactcat gttcgacgtg aataaattgt ccgtcgtatc atgtgaaaga attactaatt 6387actactattg ttgactagtg gatctttata agtttatgta catttgaaag ttaaaagaaa 6447cataaagata tataattcat cgatgcgatg ctttagatat tatgatatct tgttaacgaa 6507atgatttact catttggtaa aattgcataa gaattgaatt tcaaagtttt cacaacttgt 6567gcattttcat atttattaga aaaaatataa cttcatgtcc gacccaatac ccaaccataa 6627aattccttaa ttatatgcta agcaaatatc atataagtgc ataatttaac tatgaaactg 6687ccaaaccaaa taaaattttg ggggattttc ctaaagagtc atttggtgtg aagtatgaga 6747tataataatt ttgggataaa atgcagaatt attttatctt gtgtttggtt ggagccatat 6807ttggtagtcc tccgattatg tatcccatat catagtgatg gaatatataa attatgatca 6867catatacatg atcaagtaac ttatttcgcg ataattaatc ccgaaataac ttgctccaac 6927caaacaaccc ctaaagaaaa agacaaataa tatgggtgta ttattgagtc actttacagc 6987taccgactta attcatctct tgtt 7011371245PRTSolanum tuberosum 37Met Asn Glu Asn Glu Ile Glu Glu Met Leu Asp His Leu Arg Arg Ile 1 5 10 15 Lys Ile Glu Gly Asn Leu Asp Phe Phe Lys Ile Arg Arg Ile Gly Asp 20 25 30 Leu Asp Ile Val Leu Arg Val Phe Arg Thr Phe Ile Lys Tyr His Val 35 40 45 Leu Leu Pro Asp Cys Phe Val Lys Leu Thr Met Asn Ala Glu Trp Thr 50 55 60 Val Glu Met Leu His Arg Val Phe Asp Gly Ile Ser Asp Glu Cys Lys 65 70 75 80 Thr Asn Leu Asn Leu Glu Arg Leu Glu Ser His Leu Leu Glu Phe Phe 85 90 95 Glu Gly Asn Ser Ser Leu Ser Tyr Asn Tyr Glu Leu Asn Asp Phe Asp 100 105 110 Leu Ser Lys Tyr Met Asp Cys Leu Glu Lys Ile Leu Asn Asp Val Leu 115 120 125 Met Met Phe Leu Glu Lys Gly Arg Ser Cys Tyr Pro Ile Glu Lys Leu 130 135 140 Ala Ile Gln Leu Ser Ile Lys Lys Leu Lys Ile Val Gln Lys Lys Met 145 150 155 160 Ile Phe Leu Arg Tyr Ile Tyr Thr Thr Glu Ile Asn Gly Asn Val Asn 165 170 175 Tyr Glu Lys Leu Glu Cys Leu Glu Thr Arg Ile Gln Phe Ile Ala Asn 180 185 190 Thr Val Gly Gln Phe Cys Leu Ala Val Leu Asp Tyr Val Ala Asp Ile 195 200 205 Glu Phe Ser Asp Asn Asn Asp Ile Phe Asn Ile Pro Pro Tyr Leu Leu 210 215 220 Ser Leu Ile Val Phe Val Glu Leu Glu Met Lys Lys Ile Phe His Gly 225 230 235 240 Glu Leu Lys Val Ser Lys Phe Thr Gln Ser Lys Thr Phe Lys Asp Lys 245 250 255 Lys Leu Pro Lys Glu Phe Ser Asp Leu Leu Gln Tyr Leu Leu Met Tyr 260 265 270 Leu Arg Asn Glu Lys Leu Glu Asn Phe Pro Asn Asn Ile Ser Ala Gln 275 280 285 Asn Ile Asp Val Ala Ile Glu Phe Leu Leu Val Phe Leu Asp Ala Asp 290 295 300 Val Ser Asn His Val Ile Asn Gly Asn Trp Leu Asn Glu Val Leu Leu 305 310 315 320 Lys Val Gly Ala Ile Ala Gly Asp Ile Leu Tyr Val Ile Gln Lys Leu 325 330 335 Leu Pro Arg Ser Ile Asn Lys Asp Asp Thr Ser Lys Ile Ser Phe Cys 340 345 350 Ser Ile Gln Ile Leu Glu Lys Thr Lys Asp Leu Lys Ala Gln Val Glu 355 360 365 Thr Tyr Tyr Lys Ser Leu Lys Phe Thr Pro Ser Gln Phe Pro Thr Phe 370 375 380 Gly Gly Leu Ser Phe Leu Asp Ser Leu Leu Arg Lys Leu Asn Glu Met 385 390 395 400 Ser Lys Ser Lys Ser Gly Leu Asp Phe Leu Met Lys Pro Leu Leu Gly 405 410 415 Asn Leu Glu Lys Glu Leu Ser Ser Leu Thr Ser Ile Leu Glu Lys Glu 420 425 430 Leu Ser Ser Ile Phe Arg Asp Val Val His His Glu His Lys Ile Pro 435 440 445 Lys Asp Leu Gln Arg Arg Thr Ile Asn Leu Ala Tyr Glu Ala Glu Val 450 455 460 Ala Ile Asp Ser Ile Leu Ala Gln Tyr Asn Ala Phe Leu His Ile Phe 465 470 475 480 Cys Ser Leu Pro Thr Ile Leu Lys Glu Ile Lys Gln Ile Asn Ala Glu 485 490 495 Val Thr Glu Met Trp Ser Ala Asn Ile Pro Leu Asn Pro Arg Tyr Val 500 505 510 Ala Ala Pro Phe Lys His Leu Pro Ala Arg His Ser Asn Leu Val Thr 515 520 525 Asp Glu Glu Val Val Gly Phe Glu Asn Lys Ala Glu Lys Leu Ile Gly 530 535 540 Tyr Leu Ile Arg Gly Thr Asn Glu Leu Asp Val Ile Pro Ile Val Gly 545 550 555 560 Met Gly Gly Gln Gly Lys Thr Thr Ile Ala Arg Lys Leu Tyr Asn Asn 565 570 575 Asp Ile Ile Val Ser Arg Phe Asn Val Arg Ala Trp Cys Ile Ile Ser 580 585 590 Gln Thr Tyr Ser Arg Arg Glu Leu Leu Gln Glu Ile Phe Ser Gln Val 595 600 605 Thr Gly Ser Lys Asp Lys Glu Asp Glu Val Gly Lys Leu Ala Asp Arg 610 615 620 Leu Arg Lys Ser Leu Met Gly Lys Arg Tyr Leu Ile Val Leu Asp Asp 625 630 635 640 Met Trp Asp Cys Met Val Trp Asp Asp Leu Arg Leu Ser Phe Pro Asp 645 650 655 Asp Gly Ile Arg Ser Arg Ile Val Val Thr Thr Arg Leu Glu Glu Val 660 665 670 Gly Lys Gln Val Lys Asn His Thr Asp Pro Tyr Ser Leu Pro Phe Leu 675 680 685 Thr Thr Lys Glu Ser Cys Gln Leu Leu Gln Lys Lys Val Phe Gln Lys 690 695 700 Glu Asp Cys Pro Pro Glu Leu Gln Tyr Val Ser Gln Ala Val Ala Glu 705 710 715 720 Lys Cys Lys Gly Leu Pro Leu Val Val Val Leu Val Ala Gly Ile Ile 725 730 735 Lys Lys Arg Lys Met Glu Glu Ser Trp Trp Asn Glu Val Lys Asp Ala 740 745 750 Leu Phe Asp Tyr Leu Asp Ser Glu Phe Glu Glu Tyr Ser Leu Ala Thr 755 760 765 Met Gln Leu Ser Phe Asp Asn Leu Ala Asp Cys Leu Lys Pro Cys Leu 770 775 780 Leu Tyr Met Gly Met Phe Leu Glu Asp Ala Arg Ile Pro Val Ser Lys 785 790 795 800 Leu Ile Ser Leu Trp Ile Ala Glu Gly Phe Val Glu Asn Thr Glu Ser 805 810 815 Gly Arg Leu Met Glu Glu Glu Ala Glu Gly Tyr Leu Met Asp Leu Ile 820 825 830 Ser Ser Asn Val Val Ile Val Ser Lys Lys Gly Tyr Asn Gly Lys Val 835 840 845 Lys Cys Cys Gln Val His Asp Val Val His His Phe Cys Leu Lys Lys 850 855 860 Ser Arg Glu Glu Lys Phe Met Leu Ala Val Lys Gly Gln Tyr Ile Gln 865 870 875 880 Phe Gln Pro Leu Asp Trp Lys Gly Ser Arg Val Ser Phe Ser Phe Ser 885 890 895 Glu Glu Leu Ser Lys Phe Ala Ser Leu Val Ser Lys Thr Gln Lys Pro 900 905 910 Phe His Gln His Leu Arg Ser Leu Ile Thr Ala Asn Gly Gly Glu Ser 915 920 925 Ile Asp Val Ile Pro Val Cys Gln Ile Asn Glu Leu Arg Leu Leu Lys 930 935 940 Val Leu Asp Leu Ser Ser Tyr Tyr Val Glu Ser Leu Trp Leu Ala Arg 945 950 955 960 Leu Asn Pro Leu Asn Gln Leu Lys Tyr Leu Ala Val Trp Ala Gly Thr 965 970 975 Phe Tyr Phe Asp Pro Gln Ser His Leu Pro His Ile Glu Thr Leu Ile 980 985 990 Val Thr Ser Cys Phe Tyr Gly Val Arg Leu Pro Val Ser Phe Trp Glu 995 1000 1005 Met Glu Lys Leu Arg His Val His Phe Ala Gly Ala Gly Phe Ala 1010 1015 1020 Met Gln Gly Leu Phe Glu Gly Ser Ser Lys Leu Glu Asn Leu Arg 1025 1030 1035 Ile Leu Lys Lys Ile Glu Glu Phe Pro Ile Asp Arg Leu Asp Val 1040 1045 1050 Leu Ser Arg Arg Cys Pro Asn Leu Gln Gln Leu Gln Ile Thr Phe 1055 1060 1065 Glu Asp Asp Val Glu Pro Phe Cys Pro Lys Leu Glu Ser Leu Thr 1070 1075 1080 Gln Leu Gln Glu Leu Gln Leu Ser Phe Val His Pro Arg Ile Leu 1085 1090 1095 Ser Gly Leu Gln Leu Pro Ser Asn Leu Asn Lys Leu Val Leu Lys 1100 1105 1110 Gly Ile His Met Glu Ser Ala Ile Ser Phe Ile Ala Glu Leu Pro 1115 1120 1125 Ser Leu Glu Tyr Leu Gln Leu Leu Asp Val Cys Phe Pro Gln Ser 1130 1135 1140 Glu Glu Trp Cys Leu Gly Asp Ile Thr Phe His Lys Leu Lys Leu 1145 1150 1155 Leu Lys Leu Val Gln Leu Asn Ile Ser Lys Trp Asp Ala Ser Glu 1160 1165 1170 Glu Ser Phe Pro Leu Leu Glu Thr Leu Val Ile Lys Lys Cys Asp 1175 1180 1185 Asp Leu Glu Glu Ile Pro Leu Ser Phe Ala Asp Ile Pro Ser Leu 1190 1195 1200 Lys Gln Ile Lys Leu Ile Gly Ser Trp Lys Val Ser Met Glu Ala 1205 1210 1215 Ser Ala Val Arg Ile Lys Glu Glu Val Glu Glu Ile Glu Gly Cys 1220 1225 1230 Asp Arg Ile Asp Leu Val Arg Arg Ser Arg Arg Asp 1235 1240 1245 38174573DNASolanum tuberosum 38ctattattta ttgctaccag tagtacagct tccagggaat acagaaactt ctctatgacc 60tctaaaaaga agttaaacag aatgccaact aaaattatga ttaaggagga taaatagaga 120atgttgtgac atacacagac aaggacgcca aaaagcaact ttcaggaaaa ttcaaaacaa 180aaatgagttg atctccaaga tagttctcaa gacattcaag ttcacaccat cgctctttct 240ttcagcatgc ctctaggctc taaaattcca attaatatga ttgattcagt atttaattgt 300tttcaacata gaattgaata caaaagctgc acccagcttc caaagctaca tcactgccaa 360tatcttgacc attcgtatta ctgattaaca caatactgat caagacccat gagaaacagg 420gtaaaaagaa cagaatacag tgtcaaccac tgttctcatt aaattaacta tgttggagca 480ctgatacatg aatcaatctc tcttcttcct gtaagtgcta taaacttatc cctaaataat 540aacagagggc atttacagat caaattagac ccatcataag cctctttaag tatcacggta 600ctcctaacac ctttattagt gatgtagaat atactgtgtt gtttgattaa gcaaagcaaa 660agtctagctg gtaaccgtaa ctcggaatga aatgcatcta tttgagcaga tgtcaacctc 720ttttccatag tcaaactgag gagctcgtga agcactgcga ctactctctt ttgagctttg 780ggatcagcaa gttcaaatct tttagcattc aagtatggag aagggaactc catcttctgc 840catttctgaa cttccttaag gtagttcata ttcggcctaa atcctgcagg ataatgcatt 900tgaaacgaga agggaccgaa atagtttcca tctttcaata ctctaacctt ctcttttgaa 960gtcaggaccc cttcacacgc

caacctttcc tcccgggcag tgacagctag aaaagaatcc 1020caattttcca actgaaggga agttcttcca ttcacgtctt tcactgagaa aaattgtggg 1080tattttggaa tcagcgactg cctgaagtca ttaggcaagc ccaaatcatt ctcaatcagt 1140tgaatggttt ccagtggcaa cgtgcaatca accgacaaca ttaacaactt ccttagattc 1200ttgaccaaaa taggttccat ttgcgctctt gcttcatctt cttctagtgc tattttatca 1260gccttttcag tcagcataac catcggtgga gttccattac caccggtgac acggaatatc 1320gtaggatatt tctcaataat acccatgaaa ttccactttt gcacaaatcc aacttctttc 1380tcaaggtctc taagaaggac gcattgtttt ctctgtgatt gaattatgga ttttaagcgt 1440aaaatcaatg atggtttctt ctgaagctcc atgactctgt ctagatcatt tactctatag 1500tacattttct tcttaggtct cctccctcca ctggttttga acataatttg aacccacata 1560tgccctactc ccagagatgg caatggatgg cacaatcttt gccttccatt gactgtaaac 1620cattttcgag gctcaattag cgagaagacc atcacattta tgcatctgga aaagaacaga 1680gatgtgcgca atttagctaa aacaattgaa gtgtagtaag attttggaga aaagagttgt 1740ttccccttaa aacaaaaact acaaacagag gcatatcaag aatttgaagt ttgtgggtcc 1800aagcccaatg gatggtgacc aacgaattcg aacccttgac caagagggca acaaagctta 1860aaagattaca tttttaccca ctacccaaca tccccaagcc gtttctctta ctggattttt 1920caatactccg cgcaaaattt actatgttct tgggaacccc ctccaatacc ctaaattccc 1980cccccccccc cctgagtcta caaacaggaa atgatataga cagagtcatt gagcttggac 2040tatatatgac caaaaaaagg acaaattttt ctcgactgct actacttata cgcagattag 2100tgctacacta taagaacatc atgctttaaa tttgtgaaaa aaaataaaga aatgaagtaa 2160tacaatagag ttgcagctct tcgataaatg ggaacaaata catcattaaa cacaaaaaaa 2220aaattcactg gaattcatgg tttactaaat atactcaaat tgcaagtaaa ttaaaagggc 2280ttcggtcaaa ttaaatagtg attgaagaga caaggagaaa cttacaagac ggtgtggagc 2340gcagcagagc agtaagacga atgaaaatgg cttatttgtt tttcaggttc aactttgcct 2400tttataataa tcataatcta aaattaagaa ggggtaaaat tggaatttta ttttaatttt 2460caaattgaag ggccaaaaac ttttttcttc gactccaatt ttataaagtt aaaaaataaa 2520taaaaaatat ttgaaattta tgataaaaaa tttacttaaa tatgtagagt tagagtattt 2580tgagccgttg atctattaaa tacaagtgca tctatgagcc aatttgctaa tattaagatt 2640taatgaaaga tagaaagatt gtttttgaaa gaatgaaaaa gaaaatatga tagtgaaaaa 2700aatataacta attaggaaag caaggtgaaa attaaaagaa ggtcaaagtc atatgtagcc 2760cctcaaagtt gtcttcatta ttcatttaga cacctcaact acacctagta cctattgaac 2820acctctacca ccccagattt gaaccatcta agcatttttt tttatcactt ttcataaact 2880gtgtggcgcg tgtatttaac ctccccacgt gtatccgacg tgtatagaat gaccatttaa 2940caactaccac gtgtatccga cgtgtatttg taaatgtctt aattggggaa gattcctaaa 3000tcgttaaaat tgacagaaca cttctctctc tctttgcgat tttttcccaa ttgaagctct 3060ccatcttcgc gatttcctcc agattgaagt tggattcgtc ttgttcgtgt tacaatcttg 3120aagatttcct tgtgtaagtt aggattgtta atgattttta catgctttgt taaatgaatt 3180ccaaaaaagc tagggttttg taaaaatata gggtttgtga tttttgcgaa tattctgtcc 3240cttctttact attacccaaa aaatatgctt taaaactttg gtttttagtt gttgtatatg 3300ttatatgagt atattccgtt atcttaagct agggtttgtt tgatattttg tagggctaac 3360aattgaccat ggaggagagc cttatattta caaagattta ccatggtgga atcttatccg 3420agatatttgt ccttacttat gttggaaact gtgtgtctgc actcaggtat attattaagg 3480accacttttc cattttggaa ctactgtatt atactaaaga attagggtat gaaactgtta 3540gaggctttta tgtgaaggat ttattgaata aaaaatgggt cctaattaca actgaccaac 3600atctcctaca cctaattaaa gacttaaaat atgaggacac ttttgaagta tttgtctgtc 3660atgtattaga tgaaccttac ttgacactga gggacctaga ggctatctaa ctaatgttgg 3720tggtgaaggt gttgatgttg accttggtga agagggtgag actgttaact taggtggaga 3780aggtgaggtt gttgacctaa gtggagaagg tgaggctgtt aaccttggta gtgagggtgt 3840tgatgttaac attggtgaag agggtgtgga tgataaccta ggtagagatg gtgtggatga 3900taacctaggt ggagaaggtg aatctgattt tttgagtagt gattcagatc tagatatacc 3960ttcagaagat ggttcagata ttgatgaaga gttgagagct tttagacaag aaagaagaaa 4020caaaaaacag agaaacaagg ctactgaatt tgaagaaata ccagttggag aggctggtgg 4080tatagataga ggctttgagg atattgaaaa aaataaaaca gacaaatata caggaaaatt 4140aggtggagat gaggattata ttgatagctc tgactgttgg agtgatgata gtgatgaaca 4200actaaatatg gatgctgtta ggggagtaga tatacctact agaaggagaa gcaaaaaagt 4260taggtatgat gaagattgtg aagtttcaat atttgagctt ggaatggtct ttgagggtgc 4320aaatcagttc aggaaagcag tggcagatta tgctgtagag tacatgaggc agataaagtt 4380aagacctaat gaaaaacata gagtgaaggt gaagtgcaaa aatgctaact gtaagtggtt 4440gttatatgct tccattgaca gggactcagg tgatttcatt gtgaagaact atcatcctgt 4500tcacaagtgt attccattaa acaggaacaa gttgtgtaat tcaaagttaa ttgctagaaa 4560gtttaaggac aaaattgtat ctcaaccata cataaggatt tgagaaattc aagatttggt 4620tagaaagaca ttgggtcttt atgttggtaa gactctttgt tacagggcta aacagaggat 4680tatgaaggaa aacaacatgg gtgattggaa tgtggaattt gccagattat gtgtgtaatg 4740accctaaagg tcatttttgg aaattttcat aaaatgaccg ttttacccct cccgatagtt 4800gccccgagtc ctaattgttg tattttcgaa gttggtttgg aggaaaattg atgaaaagtt 4860gagtttttgg aaagttgagt ttttaagagt taaagtttgg ttaaaagtgc tatttcgagt 4920catttggagt ttcgaaactc gaattggatt ttcgttgatt ccagcagttt tagaatgtcg 4980aaacatgtct atgagaattt acggagtcga atttggagtt aaaacgaaga tttgaggtcc 5040taagttgcaa aaattgtgaa attttgatca agagttgact ttggtcaaca aacgaggttc 5100cggtgctcga aatggaatcc cgagggcacc gttggattca gggggtgatt ctaagtctag 5160aatgagtctt ggttaaattt ttagaggccc cgagtgcgtt tcgagttttt gagcctaaag 5220ttagtttctt gacgccttat cggataccgg gtcaaggaga cctcgaattc aaattccgac 5280gatttcattg agtccgaaat gtcattttca agctagtagc atatttggtt tgtgttcggg 5340aagtgccaaa tgaattttgg gtgagctttt aagcttcttg gatgctttga cactatttca 5400gcaaattgtg gcaactaaag ggttcattat taggtttaaa ggtcgaaatt tttttccgaa 5460ggttctgaaa ttttgagcat gaattatagg actttctgca aattttggtg cattttcgct 5520aacctgagat tggactttta tcgcaaataa gaaataattg tttacagagt agaaatgata 5580tttgactggg caaagagcat gaaattgagc tccgattgaa cgagcaggtc cgtatcatta 5640tttaagacat ttacaaaaaa gaatcgggtc atttcactat cgtatgagga aattacggcc 5700tttttagtga aagattaact gctgtttttc tggtgcataa cagccatgta ctgttataaa 5760aatctcagac tgtgttcatt tggtattttg agcatatcgg gagttctaga gatcggaatg 5820gagtgattct tacgggtctc ttcttgaatt aatatgaggg ttagtattca atcttcaatt 5880cgacattttc tcataatttc tttatctggt tttttttcct atccgtaaat ctcttgggaa 5940aaattggggt tttatcgatt tggactccct ttttgatgaa aaaggtatat ttacaatcct 6000tatgttatgg gtaaactgat tttaacataa aattattgat tcatcgatta ttttcatcat 6060attaaccgcg tacaatttaa actttcccgg taaagttaaa gtttgataaa ttgagaattt 6120caaggtcgat cttaactcca tttttgatga aatttcatat ttgaacttat ctaagcatgg 6180gtaagatgtt tttcaagaga tatttcattt tcgagtcggg gtttcggatc cgaatatttt 6240aggcttcttc aagaacgtgg attttccttc aaattgagtt tgtgaattga tttcaactcc 6300gttttcaaat tgggtttcac cattagtttc caaatactgt aaggatcatt ttacataaaa 6360aaatttccat atttgggtat cattttctgg tatgagactt ttggaccttt ttgccctttt 6420cccctaaatt tcttgatttt ggtgtcattg gactcgaatt gtgattgtga ataattgttt 6480gaatagattg tcgtgatccg gattatactc gaaaaggaaa ggctcaagtc aagtaacttt 6540tggagttcgt ttcaaggcaa gtggctttca aactttgtaa aactcttaga ctacgcatga 6600ctactttcct aattgtgttg gggagtaatg ggtattgagg atgagtttta tttgttgatt 6660gaaattgttg taaatgaaag atggggaata aaacgagcta aatgtgttat atgtgacttg 6720aatttgttaa ataagtcatg tgataactga tattgagggg atagaagagc atgagtaggc 6780tatgattgat acagacattg atgttgtgac atatgatgtg taatactatg atgtggtcgt 6840gatatggttg tgattgagac aggtgatgtg taatactgtg atgtggtcgt gatttggttg 6900tgattgagac atgtgatgtg taatactatg atgtggtcgt gatatggttg tgattgagac 6960agatgatgtg taatactatg atgtgatcgt gatatgattg tgattgatga catgtgcata 7020ttcattattc atcccatgtg tgaactatct gttgcatgag ttctgagaca ctgatatgag 7080gatggatgga tatgagacat agttgagact agctccggct agcgatttgg atgccgatgg 7140gatctggttc cggcggtgat acatggtcca tgtgtggccc ccatgggttc tgatttgagt 7200attcagcgcg gactgattac gtcaacagat gtgtatcgta ggacaaacat gcatcatgac 7260atcattattg cattttgcat cgcatttgcc ttatctttgt ctgtgatgtg tggattgtat 7320cggtttaccc ttcttatgtg gaatttgatc tacttgctct tatttgttga tctgaggttg 7380atgaggatat actgttggtt ctggctgttg aatatgatct gcttagtata ggttggttgg 7440tttgctgcta gattgaagtt tcggtggttt ggttgggatt gaaatgagtt gtttgtagct 7500gctagttttg cttagtttag agttacttgc gagtacctgt ggtttttgat actcaccctt 7560gcttctacac aattgtgtag gttgacagct ctctctcaga ttcggcttag tagtttcttt 7620agcagattga gcttcgggac atactcgaga ggtagcggtt cattccagac gtgcccttga 7680gttatcttta ctttcagttt tgttctattt gagaactata ctctgagact tgtatatttt 7740tattcgaatt ctgtatttag aggtttgtac atgtgacaac caaattctgg gtagtgttga 7800gtcttaatta aagtctttcg cttatttatt atcttttatt ctcgtatttc tacttctcta 7860tcgttgtggt tgggttaggc tgatgtgtcc ggtgggaaat ggatacgtgc catcacatcc 7920ggatttgggg tgtgacaatg tgactatgca gatatgatca aacaaaccaa tcctggaagc 7980tcttgtcggg taaaaattga taaggaaact gaaccaggaa agaacctttt tgtgtatttt 8040tatgtgtgct ttcatgcatt taagcaagga tggttggagg ggtgtaggaa tataattgga 8100gttgatggtt gttttcttaa gggagcttgt aaaggtgagc tactagttac tgttggaaag 8160aatgggaaca atcaaatgta tcccattgcc tgggaagttg tagatacaga aacaaacata 8220gttggagctg gttcataagg tatttaattg ctgatctaaa cttgggaagt ggtgaaggtt 8280tgactgtaat gtcagatatg caaaaggtat tgattataca atttttatat tttagtttaa 8340tttgatcttt acttcttcaa ttacttattt tgtacatatt ttttgttagg gacttattcc 8400tgtcttgtca gagttgttac caaatgctga gaaaaggatg tgtgctagac atatatggag 8460taactggcat gtgaactgga aaggagaaga aagaaggaaa cagttttgga gatgctcaaa 8520ggcctccttt gaagtcaagt ttggagaaga agttcatgca atgtcaaagc taggtaagaa 8580ggaaataaca gaggacttgc tacattatga tcccagaaat tagtctaggg cctttttcca 8640aactcactcc aagtgtgatg ttgtagaaaa taacatgtgt gagactttca attcctggat 8700cttagctgct agacataaat caattattac catgttagaa gatattaggc ataaaatgat 8760gaacaaacat atagacatga tcaaatttgc tgaaacatgg atatcagaca ttgcacctat 8820ggcaagggca atactagaaa gaaacgagga gtattcaaat aattgtaatg ttcaatggaa 8880tgggttgaat gattttgaga ttagtgaagg gaatattcat ttgttgttga cttggagaag 8940aaacattgtg actgtaggtt gtggatgttg agaggtattc cttaccctca tgctatttgt 9000gcttattatt acttgaatca agatcctgat caacatgtag agcactggta taagaaggaa 9060acatttctca aggcttacat ccattttatc caacctattc ccaatatgag gatgtggcct 9120gaaactacaa atccatcaat agagcctcca aaaccaagaa aaatgccagg cagacctggc 9180aagaagagaa gaaagagtaa ggatgagcca aaaaaatggg ggaaactttc aagaaaagga 9240gtaaagatga catgtaccat atgcaagaaa actggtcata acaaagctgt gtgtgcaaga 9300gtaagtaata cttcatattc acatttgtaa attgcttaaa gtttatatat attataatca 9360tatattttat tttgtggtga catagtatac gagggggagc acttctcaac ctacaagtca 9420aggaaattca agccaacaaa gttattctca aacaggagta agatttgatt tacatattca 9480tttgcattaa ttttcatcgt ataagttatt catatctcct tatttttgtt caataggtta 9540gaacacaatc aagttaacaa agttctgttt gcgctgatac cactgttgtg ccaagaacta 9600ctcaaacaac agtaagatta gatttaataa ttcatttaca ttttctgatt gatctacaag 9660ttattcatat atccttattt ctgctatata gtctacaatg ggaggtccat cgcattctac 9720tttcagaacc acatatgcaa ctacataatc aagtcaacca agttctattt gtgctgacac 9780agaatctgtg ccaagacgtc ctcaaaatag ggttcaagtt gggactggaa gaggattggg 9840aagaaaaaaa gctaatgcaa gagtgatagt tcttctagtc aattgccacc tttatcaggt 9900cacaaaagac cgtacaattc tgcctcattt gctgctgctg ctactggagg taacagaagg 9960cttgcaactg gttttggtgt ttactctaat cctactactg gagcccaagt atttaatgta 10020tgtttactct gttttggtgt atattctgat agctatatta tgatcttacc ttatcttaca 10080ctttctttct tatattacag cctggtacat caagtgagaa gattctacat gggccaacaa 10140aattgaagag tgcttcacca accaatatag acattgtctt taaacctcgt ggcctaaaat 10200ggaagggaaa agatgcagtc agcacctcac agttgcaaca aatgaaagca aacaggaaaa 10260actaaaagta gttcatttgg aaagttgtta tgattttggc ccatgttaaa agggtcttca 10320acaagctact ctttagttta ttttgaacac tcgtgtgatg tatttttgaa ccatgccttt 10380gtgttagcaa gactatgtat ttttgaacca tgcaagacaa tagtttttta ttacctctca 10440atgcatttgt gttatgtatt tttcaaccat gactttgtgt tatgtatttt ccagccatgc 10500atttgtgtta ttagctcagt ttttgaacaa caactacaca gggcaagtcg atcccagaac 10560taatttcaga acacagggca agttagaaca cagggcaagt tagaaaacag aggcaagtta 10620taaaagagaa actgatcaat tcacactaga agttacaaaa gagacactga tcaattcaca 10680ttcaaacttc caatttgata taaaatatga acttaacaga ccaattaagt acaacactaa 10740ttacgtgaaa taacttccta actattgcaa aactaaacaa ttaagtacta caattttgaa 10800ctacacaata ttaaagaacc attttatagt tagcagacaa agagaaacaa tcacaactcg 10860attaaatttg acattttccc aatccctttg gagtttaact tccttgagct tcttgttcaa 10920taccatcatc ttctcttcat tttcattcaa ttgaaatttc aaaaaatccc tttcttcttc 10980aagctctttc acttttctct ttagttgatc aatcggatta tgaccatcta tgaatctatt 11040cattaaggat gattcatcaa tattttcaag tgacggatca atccatctaa aatagccaca 11100tccaccattt tcctacaaac cacataacag taataaatat acaaaaatgt tgatttttca 11160aataatacaa taatagtagt ttttgttcaa ataactacaa acctttgatg ctttgcatcc 11220aaaaaatcga caacctgggt tcaatggagt cctcgaagtt ttcagtcgac aatagtaacc 11280acacttacat acatcgactt cctcgatatt cactgaactt tgtgacattt gataaaacac 11340aactgattta gaagagagaa acgttggata tttagaggaa gaagagagaa ttggggatta 11400gggttctaaa atgtatttgg gggaagaaga agattttaat gttaaaatat ccaagttaac 11460gttggatgtc ttaaatgcag ggtaaaaaat gggagaaaaa acaattcacg cgcccgattt 11520gcgtgaatca caaacgattt gccatgtagg caaaaaatgc ttagatggtt caaatctggg 11580gtggtagagg tgttcaatag gtactaggtg tagttgaggt gtctaaatga ataatgagga 11640caactttgag gggctacaga tgactttggc cttaaatgaa tttgaatcat aaatcttttt 11700tgagaagatt gcaagtatta catatacaac attaattgtg cttgatatat ttacaaaaag 11760gaatctaatt ttaacctgat ttaagtctct acatgataaa ataatgagca actttcacat 11820atagcaaaca taaaaatcat atttgtatgt tatagctata gtttgtataa ttgcgctcca 11880taacaaattt tatgtttgct attgaacttt tgatttgtat aattcgctac aaacatccaa 11940ttttatacaa attgttcagt tttgtataaa ttcatttata cattgtaatt tgtataataa 12000aatctgtatt tatataatta taagtgtata ggacgaaaat atatttattt gtatttgtat 12060atacactttt ctctcgcttt atacaaacac aaacgcattt tatacatttt ataccgaaat 12120gtataaaatg actaattgta taccgaaaat ggctaattgt atatgacaaa aaacatggat 12180gtttgctgct aattacaatt aaaataagct atggctataa catttaattt aaattaatag 12240tttgctattt catacaattt tccctaaatt aattcatctt taccaacaaa agtatataga 12300attttttgcc ttttatcatc tctatagaac tgactgactc tatctttaaa gcatcttgca 12360tgttctcttt ccaaatggtt attggctctt caaaattaac tttggctggc aacatgaata 12420tgacaacatt tttgaagaat ccaagcaaca taagttctct gccacttgtg ctcttttggc 12480atgcccttct gtaccagatg tcttcaggta ttgtgttttc aagcctaaac cttatgcctt 12540catttgagaa gcagaaattg tgttatggga accaaaagaa acacaatgaa ctgacccttg 12600agaaaactta tccagccact ctatggttgt ctcctcgatc ccaagcaccg aagcagtctg 12660ttgcaaaatc tacttttgta actgagacat tggaaaaaaa aactatttaa gaaatattcc 12720atgaatgaat gattttggtt tcatgaagtt tttcttaatt ttaaacatat aacacagtgc 12780acataatgtt acatgaatga atgaatgatt ttgcacttac cataattgaa atattcatac 12840ttgttaagga cttggaggct atcacatcct gtaatctctt ccacttggtt tttaatattg 12900aaagctgaaa gcttaagtga ctcatcgcag caaatcaatt taatcaactt cagtgttggg 12960atatccacga agctacaagg gatctgaccg cactgaatta tgcaattgtt tataattagt 13020tgctcaagca cggggaaaga ttcttctgag gagcgccatt ctgtcacgag aacattctgt 13080aagttcaagt atttgagtgc tcgaaactca taatcactga catcccaaca tatgctatat 13140ccagcctgcc catatctatc ttcagacttg aagtatactt cacgtagttt gagactctca 13200aggtttcgta gtcttgcaag ttgtgaaaca actttttcag ttaggcaaag tctatcaagt 13260gacaaatcct tcagatttga agggaagaca acacatctgg cccgtcctat ggatttgtag 13320cctgaaatcg cgcgtgagaa acacagttcg agagatagaa gtgggagttc ttcaagattc 13380ggcatatcaa cttcataacc tggttcaata aagtggaggt tgagttgttc aatatttggg 13440aatctccacc agaactccgg agtcttatca accaagtata tacgacactt cccaaaagtc 13500ttcaagtttg gtaacacagt ttctgaagat tcttcgaaaa gtgctcgatc attgtcttcc 13560catttgaagg aaaaatcctg tatatccaca tgtcttagct tctgcatttt ccaaatagaa 13620ggtgatgtct caaagcaccc cgaaagttta tcatcatttc ccaccacctg caaagtttgt 13680agatcatgta ggtgtgatac ccactggaaa tcaaattctt gggtacgaat tgcaagatac 13740ctcaagtgag ttactacttg cactgccgta acccaagaac atctttccaa gttgacatcc 13800aacaaatgca atacccgaat aaatcttaag ttcttaagta cactgaaaag acgtatccca 13860tcacccagag aggaatgtgg attatcaatg aactcactat acctagcatt acattgatca 13920acaaactccc attccttcaa gcctgccaac attggaaact tatccaataa cttttcatac 13980tgcaacaaat gtttaacaag atcgtcatgg atatacttgc gacgtgaccg aggttcctct 14040gcaagtcggt acagcataaa attttcttcc tgaggttcct ttgcaagttg gtactgcata 14100aacttttctt ttgcaagttt tctcatgcaa aactcatgca ctacatcatg aacagtgcag 14160tactcgatct caccattatc ttctctcctt ccagaaacaa ttactaggct ttttttcact 14220agttcattca agcaaatttt agatgcttcc tccacgttct ctaagcccat gttttgtaca 14280aaattttcag ctatccaaaa ctttagcaaa ttggacactg gaaatttgta gtcttctgga 14340aacaatccca tgtaaagaag gcaaaacttt aaatggtctt ccaaataatc aaaacttgat 14400tctaatatct tcatgttttt ctcttctaaa acacgggacc ttaaatcttt tgcgatcgca 14460agccataaag aggcttccct tttctttgca ataattccag caatcaaaac gatcacaagt 14520ggtaatccct tgcagctttc agcaactcgt aatcctgtgt tgagtagctc tgggggacaa 14580atctctcatt gaaatacttt cttttgaagt aagtcccagc tctcatcaac cgtgagaaat 14640cgaagaaaat atggatcact gtggtgctta agctgcctgg ccacttcttc atctcgagtt 14700gttagcacta ctctgcttcc attttcatca tctgggaaag atagctgcaa ctcttcccaa 14760gcctcaactt cccatatatc atctaaaaca atgaggtatc tcttgttcat tagactcttg 14820cgcaacttgt caggcatgtc ttcatttcgt ataccatcca cactgcctaa aacttgttta 14880agaatctcag agcacatctt cctcaaatta tattcttttg aaatagaaca ccatgcttga 14940acgtcaaagt gtttatccac gcttggatga gtgaacacct ttttagccaa agttgttttg 15000cctagtccag gcattccaac aatcgagaca acgtctagtt cctcggtaca tccagtcaat 15060ttctgtatta tactttccgc atcttcctca aaaccaacta tttcctcatc aattgatgga 15120aaactacgag aaggggttct ttcactgctc agaacgttct ccatgctaat tatactttcc 15180gcatcttcct caaaaccaaa tatgtcctca tcaattgatg gacaactagg agaaggggtt 15240ctttcactgc tcagaacgtt ctccatgcca aatatacttt ccgcatcttc ctcaaaatca 15300agttttaact tatcaattga tggaaaacta cgaaaagacg ttctttcact gctcagaacg 15360ttctccatgc caattatact ttccgcatct tccccaaaat caaatattaa ctcatcaatt 15420gatggaaaac tacgaaaagg ggttctttca ctgctcagaa cgttctccat gccaattata 15480ctttccgcat cttcctcaaa accaaatatt tcctcatcaa ttgatggaaa actaggagaa 15540ggggttcttt cactgctcag aacattctcc atgccaatta tactttccgc atcttcctca 15600aaaccaacta tttcctcatc aattgatgga aaactacgag aaggggttct ttcactgctc 15660agaacgttct ccatgccaat tactgcagtt aggagtaaat ataactatgt tacaactgat 15720tgcacaatcg cttagaatta tttagattgc caaacaagtc aaaattcaac aatctattca 15780tagtttccta gaaaagatca tttcctagag ggaatactga aagtcaatct tctcttggat 15840actatttaga gttaatagta aaaataaaac acctaaaata tcactttttc gtgacctcaa 15900agttatagtc ttttagcaaa ttttaactct ctttgtatta acacactcca tttttaagct 15960ttcctattct tgaaaccact ttcctcaaac tacctaccaa caaaaatatt gaaagggtgc 16020gtgtcagatc cttcagatat

agtactccct ccatctcatt gtatgtggca accttttctt 16080tttagtttat ctaacaaaga atgtcacctt actactacta gaaacaattt aactttaaaa 16140tttctcttta cctactaaat aatttacaac cacacaaata tctaaagatt atttaagacc 16200tcaagtttca aaagtcttca tttttctctt aaatactttg tcaagtcaaa cggtgacaca 16260taaaatgaga tggagggagt atatttttaa ggatcccaca gggtttctct agagtccgag 16320catcattgct agctacttac tcttagtaca gttaagttta tatagccaaa aaatatggat 16380aaaatccaag ttaccttgct atatctggtg tgacattttt ggaaagaaaa aaaaattcat 16440aatcagaagt tcagaacata aactacatga tcttctcaaa taagacacaa aaatgttaac 16500ttatgataaa tgataaatat agacaaaata tatatacata aaaaaacagg aacctaagaa 16560gaaatctgac cttcaattat gagagagaag cggcagcttc ttataactta aaaggatttg 16620gaatatgaaa gcatatggtg aatgaaggag tgataatata gtaattaatc gttgagtggg 16680agtgagaaaa agacaaaaat ggtcccttat atttaatggt aagcttaaaa tagtccgtta 16740agtatgcact aattaatagt tttgagggtg ggcatggtat ggtatggtta atgttttgtt 16800attgtaattt cgattttcgg ttttaaaaac atttaatcat tatcatatca atttaattta 16860gtatggttcg gtattttaaa gtttggtttc gatagtttgt tcaacttcga ctaatatata 16920ttcgtataat agagtattat gactttgaca attaaaaaaa gatcttaatt gtatcatact 16980aacacattac acatgtaaaa atatatattc aaaataaagt acaaacagcc ccttttgcta 17040atcaattaca tttcaatcaa aatagagtag tcaaagtttc aacttctaaa catttagttt 17100atacaaatac tagattgcat atttgttagt attttaataa tatatttata cttcgatgtg 17160gtatttgata tttcggtatg ttatttttta aatacaaaat accataccag atactaaaaa 17220aaagtatacc atataccata tcaaatatca taatactgaa atcacggtat taaaattttc 17280gatatattat aataattgga tatataccat atcaagccca cccctagttt tgatcattta 17340agtaatttgt cacaagttag caaacatgat ctgttatgct tgaaggttca aaataatttc 17400ttttaagtat gcacttaaca attgtggtac cttaaattta caaaaaaatt aacaggaagt 17460ttattttcgc tagattctcg gctcaagaac aagtaaatat gtgaagaagt tgttgagaat 17520aaaactaatt aacaaaagtg tgatgttcac atatttcgat ataatatcag agtagtcaaa 17580gatcctgaaa ttgaatctta ccatcactat attaaaaaaa atcacttatt tgacccataa 17640aaaagatcgt gaaatcaaat atcaccttca ctatatataa tgaaaaagaa tcaagtttcc 17700acatgcaaaa tgggtaaaga cttgtttttt tcttttttgg atatagagag atagtagata 17760caaagacata taagctatgt atagacacat taattaaaga gcaattgtat gttacatctt 17820gaaagaacaa agagctcagc tgaccataat tttcaagtaa ttccccaaaa attcataaat 17880gtgtaacttt gtcagaattt aaaaaataaa agaaggcaga ttctcaattg gagcagtaag 17940aataggaaga aatttgaaac ttacattgaa atgagaaaga aaaccagagg tacgtagtac 18000tgcagtgcta attcctctat gtttattgaa gttcgaataa atttgaattt atgtccacaa 18060aaataacaaa gtcaatttca gatctgaagc tcaaattgaa gaagaaaaag aatctttgtt 18120tggtgcacat atgagtggtc acttggaaag agcaggcaat aagcaagtta aatattcaca 18180gtttattgaa aaaaaacaaa aaacaagcaa gttgaatgaa ggaagaatat acttcgaact 18240ttcaacaaag gcgggctctt tccagcgatt gtatgccaaa ttcactatgt gagtcttaac 18300gtattcttta attttgtaat attaaaaaat tgtggggaaa aaaaaatctt ttttaagcaa 18360actaaaaaaa tatatatcaa aacaaataga ttggaagtga aggagtgtaa ttttgagtct 18420attctttgac tccacttgga aattcttaca aaataatcga ttgaatatat atcaaagata 18480actagatcaa gtgtttgaat aaaatcttca agatatataa caacttaatt tgctcataag 18540atttcgtcca catgaaagct gactcattaa tttcgaagca tgtttcaata aatagttcat 18600aatagttcaa ataaaaaata caaacacttg atagttcaaa tagaaaactc gaaaaaagat 18660aaatatttca actgtgtttt tacaccatta tctctaaatc aaataagaca gaacttggag 18720actttcacat cctgtgatgt cttctacttc tttaatgttc agagctgaat cctcaagtga 18780cttcgtgcat ctcctcaatt taatcaacat ctgctatatt tttcatccca gaagcaacag 18840cggagccatg gtttttacca aggagtttca aaatatgaaa aaataaacac atgaagaaat 18900caaagtaatt acacatctac tatatataaa caatataact ttttgtcgaa gatgattcag 18960atgaacccct gactctacat ggatcctccc ccgcccagaa gcaaccatca tcgtccttga 19020actcgaagaa tgctccatta agtctctcaa ggaatctcac ctggagaatt cttgatatac 19080tgtggatgaa aacagaatat tagcagtaaa taagagagtg agtccacgat gctacgaact 19140agccaagcag ctcaccctga tatctccaag caaactctaa actcagtcat gacagccact 19200atcacgggta tacattactg tacaaaaaat gcaataagtg tagtatgagt acaaaatata 19260acgtgtattc aacaagtatc attgatagta atgagggttg gtctttcaaa tgcttgtgtt 19320ccatcctgat tagcatatgt gatctcaaca gacatagaat aacaacatag aagtatatct 19380aaccgaacat tgttataaaa aaaatacaca aaaccaacct acagagaaga tatgcacaga 19440ttcaacccaa caactaaata aaatgcgcga ataagagtta tgatgcgata taagtaaagc 19500tgattcttta ccacaattgt gtcaaccaat cccaggtaaa acaaccctac gcgatcacca 19560ctcctcctcc catgatcaca aagcacaaaa acctgatgca ctagtaaaaa aaaatctggt 19620gcaactgaaa taggatatta gcacccaaaa ggccaaatct cactgaacct ctcgagttgc 19680agtgcaaaca tatgtacaag ttcaaactca tcctatgaac ctactcaaat cttgaattcg 19740agcccgtctc atcacaacat tgacttcggt tctaacattc caaaattcat ctgagcctct 19800tggaacccat gccccccata cccacaagtc ataaaacacc aaatgaacat actgaaagtc 19860tcaaataggg gaaagggatt ctaaaactca aaacgaacta ataggtcatt acaaacatgg 19920gaacataagt catttgcaac ctcaagtcaa aaggcggcag tgagctacgg ttgttttctt 19980gatcccaaac actgaagcat tctgctgcaa aatctgcatc tgcatatcct ataaagaaag 20040aaagaaaaat agagagaaaa atcaaaatgt tacctgaatg aattattttt tacctaaata 20100aagtttctct caagtccaaa cagttttctg gaatactatc catggttaaa cgggcactta 20160atgatatagc ttactgtctg gttgatactg aatatgaagt tggagactat cacatcctgc 20220gatctcttcc acttcatttt taatgttgaa agctgacttc tcaagtgagt agttgcagcc 20280aatcaactta atcaacttca atgtcgggat atccacaaag ctagaaggga tctgatcaca 20340ctgaattgtg catttgttta taattaattt ctccagcaca gggaaggatg actctgagga 20400gcgccattct gtcacgagaa tattctgtaa aagcaagtat ttgagtgcta ggaactcata 20460atcactgaca tcccaacata tgctatatcc agcctgcctg aatctacttt ccggcttaaa 20520gtatatatta cgtagtttga gactttcaag gtttcgcaga cttgcaagtt gtgaaaaagc 20580ttcttctgtt aggcaaagtc tatcaaggga caaatccttt agatttgaag ggaagacaac 20640acagcgggcc tgtccaatgg atttgtagcc tgaaatcggg cgtgaaaaac acagttcgag 20700agattgaagt gggagttctt caagattagg catatcaact tcataacctg gttcaataaa 20760gtggagcttg agttgttcaa tatttggaaa tctccaccag aactccggag tcttatcagc 20820caagtatata cgacactttc caaaagtctt caaatctggt aacaaagttg atgaagattc 20880ttcgaacagt gctcgatcat tgtcttccca tttgaaggaa aaatcctgta tatccacatg 20940tcttagcttc ttcattttcc aaatagaagg ggatgtctcg aaacgccttg aaaaatcact 21000attccccacc acctgcaaag tttgtagata gtgtaggtgt gatacccact ggaaatcaaa 21060ttcttgggta caaattgcaa gatatctcaa gtgagttact gcttgcactg ctgtagtcca 21120agaacgtctt tccaaacaga catctgacaa atgcaacacc cgaataaatc ttaagttatc 21180gagtaaagga aaagtctttt cccaaacacg ggactttgga tgagcaataa acttaagaaa 21240cctatcatga cattgagcaa aagactctcc ttccttcaag cctgccaaca ttggaatcct 21300atccaatgat tttgtatact gcaacaattg gtgaacaaga tcatcatgga taaacctaat 21360atattggggt tcctttgaag gccggtactg cataaacttt tcttttgtaa gtttcctcat 21420gcaaaactca tgcactacat catgaacagt gcagtactct atctcaccat tatcttctct 21480ccttcgagaa ataattacta agcttctgtt cactagatca ttcaagcaaa ttttagatgc 21540ttcttcaacg ttctctgtgt ccacgctgtg tataaaattt tctgctatcc acaagtttag 21600caaattgaat actggaaatt gatggtcttc tggaaacaat cccatgtaaa gcaggcaaga 21660ctttaaatgg tcctccaaat ggtcataact tgattctatt atcttcatgc tttgctcttc 21720taaaacatgg gaactcaaat cttctgcgaa cccaagccac aaagaggctt ccctttgctt 21780tgcaataatt ccagcaatca aaacgatcac aagaggtaat cccttgcagt tttgagcaac 21840ttgtaatcct gttttaagta gctctggggg acaaatctct ccttgaaata ctttcttctg 21900aagcaagtcc cagctctcat ccacggtgag aaatcgaaga aaatatggat cactgtggtg 21960cttaagctgc ctagccactt cctcatctcg agttgttagc actactctgc ttccattttc 22020atcatccggg aacgataagt gcaactcttc ccaagcctca acttcccata tatcatctaa 22080aacaatgagg tatctcttgt gcattagact cttgcgcaac ttgtcaggca agtcttcaga 22140tgggctatca tccatattgc ctacaacttg tttaagaatc tctaagaaca ccttcctcaa 22200attatattct tttgaaatag agcaccgtga tcgaacgtca aagtgtttat caacacaatg 22260atgagtgaaa accattgtag ccaaagttgt tttgcctagt ccaggaattc caacaattga 22320gtcaatgtct agttccttcg ttcctcccgt caattgttta attatgttat cagcattttc 22380cttaaaacca accacttcct catcaattga ggtggaagtt ctttcaccct tcagaacgtt 22440taccatgtca gttatcttga tctctttact gctggtagaa gtagaaataa ctatattaga 22500actgattgct cgtcctagaa taaatagatt ggcaaataaa ttaaagttaa ctaatctact 22560aggtctatca aataatcagg tccatttatg taacggtagg ggtatatgtg agccactttt 22620gtaacgaggg atatatcatc tctaaatcgc aaagttgagg ggggatatga gacccttttc 22680ccttcttttt tttggggtat aagctatata taggcacaca ttatagctta gtgcattcat 22740cttcttcttt tcttaacatg aatgtagcag aactctttta tgctacatct tggaagaaca 22800aatagctcag ctgaccataa ttttgaaggt cttcacctaa tactccctca gttcactttt 22860acttgtccac tattccaaaa atagaattta atttttattt gtcaattcta acatataaaa 22920aaaagacgat tttttcccat gttttaacat ggagtattaa ttacttattt ccctaatcat 22980tttccaagac ttaatattgt aaaaatactc atatgagtca tatcaattat tgtttcttaa 23040gcatctgcaa agtccaaagt ggacaagtaa aaataaaccg aataggtaat ttcttttccc 23100aaaatttcag gactttgtaa cttctgatag aatttcaaaa accaaaaaag acacgctctg 23160cataaaattt ataaacaatt gccgaaagat tcgtaaattc aaacttacat tctcaaatga 23220gtagaaaacc agaggtacgt agtactgcaa tgctaattcc tggatgcttc tttcacttct 23280ttactccaat taaatttgga tttgtactcg taacaaaggc gatttcatat ctgaagctca 23340aagttgttga agaagaagaa tagtgtttgt tcttctacga taccaatgtg tatggaattt 23400ggaaaatact ttttcacttt caacaaaggc tgacgctata tattctttcc cttttttgaa 23460atgaaataaa agttccacta gtaatattat aagtttttat ggtatacact agatctttaa 23520ttaaatccat gtgagggatc tttttttttt tttatgtcca gatcaacttg catgcacctt 23580aactaattcc acgaaatact ttatacttgt cacctctcac caacaactca actaatttca 23640tagggtattt gttacctccc accatcaata gataccaaat aaatttgtac accaagatta 23700ggacaaatag gaagaaatcg cctagtgatt ttgtctatgt aataatttga acctcgtttt 23760ggaattcaca caatgtccaa aagagtccca aaaaggagta tcaaaaggtt aacatctgct 23820aatcatcata tcatattata tatatatata tatatatata tatgtaatga ccctaaaggt 23880catttttgga aattttcata aaatgaccgt tttacccctc ccgatagttg ccccgagtcc 23940taattgttgt atttttgaag ttggtttgaa ggaaaattga tgaaaagttg agtttttgga 24000aagttgagtt cttaagagtt aaagtttggt taaaagtgct atttcgagtc atttggagtt 24060tcgagactcg aatcgaattt ccgtcgattc cagtagtttt agaatgtcga aacaggtcta 24120tgggaattta cggagtcgaa tttggagtta aaacgaagat ttgagatcct aagttgcaaa 24180aattgtgaaa ttttgatcaa gagttgactt tggtcaacaa acgaggtttc ggtgctcgaa 24240atggaatttt gagggcaccg ttggattcag ggggggattc taagtctaga atgagtcttg 24300gttgaatttt tagaggcccc gagtgcgttt tgagtttttg agcctaaagt tagtttcttg 24360acgccttatc ggataccggg tcaaggagac ctcgaattca aattccgacg atttcattga 24420gtccgaaatg tcattttcaa gctagtagca tatttggttt gtgttcggga agtgccaaat 24480gagttttgag tgaagctttc ttcttggatg ctttgacact atttcagcaa attgtggcaa 24540ctaaagggtt cattattagt tttaaaggtc gaaaaattat tccgaagatt ctgaaatttt 24600gagcatgaat tataagactt atctgcaaat tttggtgcat tttcgctaac ccgagattga 24660acttttatcg caaataagaa ataattgttt accgagtaga aatgatattt gactgagcaa 24720acgagcatga aattgagctc cgattgaacg agcaggtccg tatcattatt taagacattt 24780acaaaaaaga atcgggtcat ttcactatcg tatgaggaaa ttacggcctt tttagtgaaa 24840gattaactgc tgtttttttg gtgcataaca gccatgtact gctataaaaa tctcaaactg 24900tgttcatttg gtattttgag catatcggga gttctagaga tcggaatgga gtgattgttc 24960cggttttctt cttgaattaa tatgaaggtt agtattgaat cttcaattcg acattttctc 25020ataatttctt tatctggttt ttttttccta tccgtaaatc tcttgggaaa atttggggtt 25080ttatcgattt ggactccctt tttgatgaaa aaggtatatt tacgatcctt atgttatggg 25140taaactgatt ttaacataaa attattaatt catcaattat tttcatcata ttaaccgcgt 25200acaatttgga ctttcctggt aaagttaaag tttgataaat tgagaatttc aaggcgattt 25260taactccgtt ttttatgaaa tttcatattt gaacttatct aagcatgcgt aagatgtttt 25320tcaagagata tttcattttc gagtcagggt ttcggatccg aatattttag gcttcttcaa 25380gaacgtggat tttccttcaa attgagtttg tgaattgatt tcaactcctt tttcaaattg 25440gttttcacca ttagcttcca aatactttaa ggatcatttt acatcaaaaa tttccaaatt 25500tgggtatcgt tttccggtat gagacttttg gaccgttttg cccttttccc ctaaatttct 25560tgattttggt gtcattggac tcgaattgtg attgtgaata attttttgaa tagattatcg 25620tgatctggat tatactcgaa aaggaaaggc tcaagtcaag taacttttgg aggtcgtttt 25680aaggcaagtg acttccaaac tttgtaaaac tcttagatta cgcatgacta ctttccaaat 25740tgtgttgggg agtaatgggg attgaggatg gattttactt gttgattaaa attattgtaa 25800atgaaagatg gggaataaaa cgagctaaat gtgttatatg tgacttgaat ttgttgaata 25860agtcatgtga taactgatat tgaggggata aaagagcatg agtaggctat gattgataca 25920aacattgtga ttgagacagg tgatgtgtaa tactatgatg tggtcgtgat atggttgtga 25980ttgagacagg tgatgtgtaa tactatgatg tggtcgtgat atggttgtga ttgagacatg 26040tgatgtgtaa taatatgatg tggtcgtgat atggttgtga ttgagacatg tgatgtgtaa 26100tactatgatg tggtcgtaat atggttgtga ttgagacagg tgatgtgtaa tactatgatg 26160tggtcgtgat atggttgtga ttgagacaga tgatgtgtaa tactatgatg tgatcgtgat 26220atgattgtga ttgatgacat gtgcatattc attattcatc ccatgtgtga actatctgtt 26280gcatgagttc tgagacactg atatgaggat ggatggatat gagacacagt tgagactagc 26340tccggctaga gatatatgag atggactagc tccggctagc gatttggatg ccgatgggat 26400ctggttccgg cggtgataca tggtctatgt gtggccccca tgggttatga tttgagtatt 26460cagcgcggac tgattacgtc aacagatgtg tatcatagga cagacatgca tcacgactac 26520atgacatcat tattgcattt tgcatcgcat ttgccttatc tttgtctgtg atgtgtggat 26580tgtatcggtt tacccttctt atgtggaatt tgatctactt gctcttattt gttgatctga 26640ggttgatgag gatatactgt tggttctggc tgttgaatat gatctgttta gtatatgttg 26700gttggtttgc tgttagattg aagtttcggt ggttcgattg ggattgaaat gagttgtttg 26760tagctgctag ttttgcttag tttagagtta cttgcgagta tctgtggttt tcggtactca 26820cccttgcttc tacacaattg tgtaggttga cagctctctc tcagattcgg cttagtagtt 26880tctttagcag attgagcttc ggaacatact cgagaggtag cggttcattc cagacgtgcc 26940cttgagttat ctttactttc agttttgttc tattcgagaa ctatactctg agacttgtat 27000atttttattc gaattctgta tttagaggtt tgtacatgtg acaaccaaat tctgggtagt 27060gttgagtctt aattaaagtc tttcgcttat ttattatcgt ttattattgt atttctactt 27120ctttatcgtt gtggtctggt taggctgacg tgtccggtgg gaagtggaca cgtgtcatca 27180catccggatt tggggtgtga caatatatat aatagagaac cttaggcttg tctacgtgtc 27240gacaccacaa accaaaattc tcttttaatt tatttattta taaaattagt cttctctttt 27300atgaaaagtt gcgactttaa tgaagagttg tgacttttat gaaaaattgt gatttttatg 27360aagagttgcg actttaatga agagttgcga cttttatgaa aagttatgac ttttatgaaa 27420ggttgtgaca tttccgaagt gttgtaactt ttccaaatag ttgtaacatt tttgataagg 27480cacaataaac atttgtccac tttacccttt gttgtcaata aatagaggga tttcctctca 27540ttttaacaca actaaaattc tgaacctctt cttcttcttc ttcttcttcc caattaaata 27600tttgtatact ttgatcatgt tgagtggctc actgacacta ttgtttatgt tacagatcat 27660ggtatatatt tttacaatca ttaatttata atttatgctt atgttattaa gctaaatgat 27720aagatgtaat aattttcatt tttctttaca ttattaatgt ttgttactat gtaattttgt 27780atgtaagata atatagtgtt ttagttttaa tgtctgatat gttttaagta ttattttata 27840aattttgtga tattaaattc tcacacgaaa cgcgggttat tttactagta tatatataaa 27900agagaacctc aggcccgcct acgtggcgtc accaaaatcc aggattccct tttaatttat 27960ttatttccaa aattagtctt ccccttttat gaaaagttgc gacttttatg aaaagttgcg 28020acttttatga aaaattgtga cttttatgaa gagttgcgac ttttttgaag aattgcgact 28080tttttgaaga gttgtgactt ttttgaagag ttgcgacttt tatgaaaagt tgtgaccttt 28140ccgaaagagt tgtgaccttt ctgataagac acaataaata tttgttcaca ctactctttg 28200ttgcttataa atatagagat tttctctcat tttaaaacaa caaaattttt gtactacttc 28260ttcttttact cctgttcagt gactcacttc accattgttt tttgtatcaa tacaatgatg 28320aatagaatcg ttatatcctg agaggatcta ttcctttaaa tctcggatac tgaaggggaa 28380taattttcct aagggtacac aatgcattta gtgggctcaa ttttttcctt tctgtttcat 28440tctattgtta catatcttga ttttttttca cagtcattaa tttatgctta ttttattaat 28500tattattttt gagtatatct tttaaaattt gttgtttatg tttcttcaaa gttattatta 28560taagtatttg ttagtacatg ttaaataaca cacgttaatt gagtattcat gcaagttttc 28620tctactaaaa ctgttgaaag tagagtaaaa gaattcctta aagatctgta ctaccatatc 28680ttgaagacaa aattcaaatt tgatatgtca ttgtaaacac ataaagatct taaaaatttt 28740atcgcacctt gatgtgcaaa gtgaaccagg aaaagaagac aaaaattaat aaacttttga 28800cataagttca aggatgtgtc ggatgaccta tagaggaaaa tgactagact gcaacatctt 28860ttaatcccaa agaaatagat aactacataa taaattaatt gctcatattt aatatgaagt 28920aaggacaaat ttctcaagtt tactgcaagt tgtaaatatt tttcttgaag taatagacgt 28980gttttcgaca ctgtgtggaa tagaagtgta gttcaaattg aactttatca ctctggtgtg 29040ttaattttta aaaaacaact agacaaattg aatagttttg tgtaatattt tttcctttca 29100agtatatata atgagttgaa ttgttgtcca atatgataca ttttccattt aatattcatt 29160ttaataatta cttacgatac atttaaatgg gaatttcaaa atgatatatg taaaacaaaa 29220gatagtgctg aactttctac acgctagaat tagaatattt gtttactttt ttcaatcttt 29280tacaatttat ttatctctac accaacaatt atttatttat tagaatattt aatactacca 29340gtttatgcat ttgattcctc aaaaatgtcg aatagtaaca cttcctagaa gcaaaataaa 29400aaataaaaaa atcaaattaa agatgattga ttgaaagata aatgataaaa tgttataatt 29460atcacttcct ttgtagtgtt ttacttaaaa tggctaatat gttataagta ttattttata 29520aatttcgtaa cattatatat ataaaagaat gttttagtgg caacattcgt cgctgctaat 29580acattaatag tcgctactaa aagaatactt tagtggcaac attagtcgct gctaatgcat 29640taatagtcgc tactaaaaga atactttagt ggcaacatta gtcgctgcta atacattaat 29700agtcgctact aaaagaatac tttagtggca acattcgtcg ctgctaatgc ctataatagt 29760cgccactaaa aatatgtttt agtggcacca tcagtcgctg ctaatgctta taatagtcgc 29820caccaaaagt atgttttagt ggcaacatta gtcgctgcta atgcctataa tagtcgccac 29880taatagtttg ttttagtgac aagattaatc tccgctaata cctataatag tcgctactaa 29940aagattgttt tagtggcaac attagacact gccaatgcct ataatgccac cactaaaaga 30000atgttttagt agcaaaatca gtcgctgcta atatcgataa tagtctccac caatagtttg 30060ttttagtgac gagagctcac ttggacaaaa ctttcgtcct tttaatgttt ttcttttttt 30120ccttctcatt caattcttct aaatattttt tgtgtgagtt attactattt tcggcaagct 30180taatttgaag aatatgaaac aaaacattct ctgaataaaa ataatatctt aagctacaaa 30240agatatgtct attaacattg ttgtccttta tgtcctaagt tattgctgaa catttgaatt 30300atctaggtta gactcatttt taatgttttt aatgttcgaa aatatttctt tagcatgagt 30360ctagaattca acattttgat ctacttgtac tttcataatg caactagaag ggctttcaaa 30420gcagcatttt aataaagaat gaatttctaa aagagtcaac aatattattt tgctgttgtt 30480tcaaattaat ttttctcctt ctataaattt tgttagagta gcaaagtaac aataacgaaa 30540agtagtgaac gtttgacctc accaaataga tagtctctaa aaaaaattat ttatgttgtt 30600gtggtgtaca tttttaacgg tcttaacgta tttataacta atattgatta tcgaaaattt 30660ttacataatt ttttttccac aaggagttat tctaaccata aaattatctg attctatatt 30720tttcatgtac aaaaaaataa aaaattatgt ttaattgcta cataaaaaca tcacttaatt 30780ttaacaacac aattatatca tcaaacatat attatgataa attatgtaaa agataaaaat 30840gattagatct caaaatattt ttaatcattc aacatatatt tacaattctt taaatatgtg 30900ataatgtcaa atcatttaat ttctgttgaa atgtttttaa aacctactaa aatttactct 30960tttttttctt ataacagtcg attattgaaa aagttgttca aaagtttaga aagattattt 31020atgcccgcgc aaagcgcagg taaattacct agtacaaaaa taaattggaa cttctctact 31080atttgtaaat ggtaaaacaa

aaaatgggaa ttatttacta ttgaaagtaa ttcaatgagt 31140tacaacattc tccttgtttt gtgattcttg tgatgttaca taattaatca attgaatgat 31200tgaatataca tagagagaag gatagtgaag tattcctttg gaaaacttca aactctattt 31260acaacttttg cagcatgaaa cataccaatg gcttgtgctt tgtcaccttt atattcttca 31320aaagctttat caagcacttc ctagacgcgc ttcagccatt tgttttgatt catcgcaatt 31380ggtccaaagg tctttcaatt cgcgtctcac tgcccccagt accacacaag ttcctagacg 31440ataaaggttt taaaacgatg aaaaaactaa acaacaattt caattaagtg gtcatcaatt 31500tcgtcatacc taatgtgcat ggcacgagat acaggagagc aggttgacca tgtccattca 31560tcaaaaacaa gccgacataa gtaatgcaaa gacctgcacg gattttaggt tgattaaggc 31620gaggaatgaa cgaaatgcag cattagaaaa gagaaaaagg agggagaaag tacaagaatt 31680aaaaagaaac acggaaagaa agaaactcag aaagaatgat agataatcaa catgattcac 31740acgtaaaaat caaaactaac atatgtggcg caaaaaacaa tttccataga tgtgcagaat 31800cataagcaga ggttttatta gaataattag aagcattgaa cgaaagaatt gtacattgga 31860gcgataatct caaaatgatt ttcaattatg aaatgaggct atttctcaat tttacccgtt 31920tttgtggtct caaacaacta agagcagcag taatgtatta agacaaaacg gatatgaaat 31980aacacaccaa caacaaaata cccagtataa tcacacaagt ggagtttgga taagaaataa 32040catagtaaga gcatataaaa gcagaaagaa caaaaagaac tacatataaa ataaaaaata 32100agaaggaaga aacaaaagag aggtacccac cagttccata accaatcatc agccaaagga 32160agtatccatt tagtaccccc ttctttttag cttcatcaaa tctgcaaaaa agatcgagaa 32220agagatcaac aagaggtcca aatgggataa atcagaagtc aaaactaaat cgccatcatt 32280ctgtagaaag aacgagtgaa tttagacaaa ctgggccgct aagttgattt gaaaacatcc 32340tctactgaat cccacaaata tttatccaat ttttgttttc ccatttttta accattgtca 32400tcggaatgat ttgtaacact tgtaaactgg aataattaga gatgtttgca taacaaataa 32460gaatttgcat tgaaacctag agcttcatat atcaaaacat tcatgactaa attcatatat 32520actcactgtc cttgtagatg cctataagaa gaatatcttt aagaacaaag gtagagaagt 32580aagtgaaata gaagtttctt atcatttaat cgaggtgatg tgctaatctt tacatttcta 32640aaagagagca acaattatct ttgcaagatg actgaagagc aaaaatacct gtatgtaaag 32700caaattagca aaccagggaa gagaatatcc ccaaagccaa gcatatcaaa tcctttataa 32760ggatctgtta atttaggaac tctcagaagc atcgggattg attctccacc agctttctta 32820cctttagcaa cctacaagag aggccaaatc caaccacccg aaaaattaga gacagatact 32880gaagagctaa attatcttct ggtaagaagt gcgtggcaag aagcgagttt aacccaactt 32940aataatgaaa ggatccagtt ttgagcacgt aaatgtaatt aacatatacc aagaaaaaaa 33000cattgaaacc atcaaaagat aaaagatgca tgtgtgcaca tttaaatcat acaggtacat 33060ctttatgttt taaacaatcc ggagcagctt cattataatc attacctagt tcctgtgcaa 33120aacaatcaaa taattttgtt gcattttgtt aatgttccac aatccacaac gacaagataa 33180atatccaaaa gtgttcaata aggttattta gctgctacta ctttgaaggt gtgcatttat 33240gcatgttgca ggaaggcaat gacatcagag taaaagaaaa ttttaatgcc caacattagt 33300ggcatatttt gaaagaacag aaaccaagta gagggaagtt ctccttccta tctaccttat 33360aaaatggaac tagaaaaaga ctgaaacttc atacagacag gtggtataac accacctgcc 33420tgtaaagggg aacaattttt aattttctag agatcaaaga ccaaaaaggc ttcatgctga 33480aaatattaag aggaagaaaa ctgggaagag ataatagcat tcttaccaag ttaatacaaa 33540taaattacaa gatacacctg tatataggtg tgaaatccat ataattaagt gtgagtataa 33600taaataaaac atggtgtatt aatgcaaagc cgaacacatt cattatgttc ataccttcca 33660ttgccaacct ctggcttcta aaaagtacca ttctcaaaac taaaaacaat aaaaatttaa 33720attaacttaa ctttcttcat ttcttataca aatctttatt tagacatcta actcatgata 33780tgatttgaca aaatatctac atcagcagta cagcttaaaa ttgtaactaa agagaaaact 33840tactgaaatc ataacactgt catggaatat agcaggagat aggaaaaccc agaagatgtc 33900atagacaaac gcgcagcaga gaagcactgt agcaacctaa aagagaaagg aaatgacaga 33960aatgaaaggt atattagtag acataggtaa agcaaaacaa gaaatcaact atgttcactg 34020gtgaatttta aaagctataa acacaccttt atattaggca attgagccaa ctgcagaaca 34080gtgatcatca aagcaatccc ctggcgaaaa aaaattttca tttactttta acagttttca 34140gctggagtaa gtatctagaa gagtgaaact tggtgataag gatgcataaa accaactaaa 34200tatccaactg caaataaagg tctatcacac aaacagtttt tcagttaagg aggagattat 34260aaaattagct tacaagaatg tcttggccaa cccaagagta tgattctttc ctgtttattg 34320cccagaagat ggcgaatccc acacaaagtg ttaggacaac tagagacaga atagcgacct 34380ccccaacaag cggcaaattc aatgttttct ttccacaacc tctaaatttg ctgcttaaaa 34440gattgtatga agcatagtta aggatctaat cagaaattct gtacataata tgaaatagac 34500ataatgggtc aatacatcat accttagtat gagcgtcact atacagttat gcagtccctg 34560gaaagaaaca aaaagttaaa caataaaaat tcaacaacca gaaaaaatgt tgaccaaatg 34620caaaccagaa ttagttttgc tccaaaacgt agaaagtgca tatgttgcac aaatcaagcg 34680taatcaaaag tagtcagaat ttaggtaagt aacctcccaa gcaccatatg gacttgcgca 34740tatcaactaa cagaaaatgg aagctaacta aagcatatgg atttgtaagt ccacatatta 34800atcacaatta caatattgca tctataagca gatggaaagg ttaacctttc agaagattat 34860ggaaggcaca attatcatgg aaaatgcatt tatgactcaa aaagttggct aaaatctact 34920gttcctacga tctaaagtta gaatcataga taacataagg tctttgatgc agccagtata 34980ttaagcctta agcctaatac acctcatcag ctggtgtatt cccttgactt ggcggttaag 35040aaagcatagc tccatgacaa tgttacccat aatgatcccc taccattaat gagcttgatg 35100agtgcaagta caatattatt agagggccac taaaaagata atatgaaaga acaatatact 35160aggcacagct gatgtgtttc aaataactac aactacaaag ctactaattc acttcgcatg 35220cccaaaagga aatttggcct ggtgattaag agtcttatag aggaaaattc aacaattcca 35280cagaaatgca tgaattttgc atatcaagaa acatttaaca tttgtagcac aattcaagag 35340tcaaatgatt gatttgaacc attaaactga tgggcattaa ttttaactat tttaaccttg 35400taatctggca caagtgaata gaaaaggtaa tccactcatg tgagtgcctt ttttctagta 35460aaatgtaaac caagtacatc tccatggcaa gtgggtgggc ctacgtagga tgttgtccca 35520gtgtgttatg ccaaaatgtt agagagctga acactggacg ataacccttt aatgatcaat 35580ttggactttt tggttggaaa acattataaa acctttcgaa ggagaggaaa acctttaaat 35640aattctaagt ttatatctat ttccaagctc tacgttttaa gttcgggaag caatgattac 35700tagtgaagac ttgagagttc cctagatatg aagaaaaagt tgttttgatc atctactttg 35760ttaatcatca tggtatgtga ttgtatccat catccaggag ctagcactcc ctgtcccaga 35820agcttctttg aatattttta tcaggggtct aaggcaggga gtgggagcat gacaaaagat 35880ttgtccactt gaatgctaga ttagaccagc tctgaaacag ttactaccga agcacaaagt 35940tgtatcattt tcctgagtag ttttagatta gcaggaaaaa ctactataaa cacggcatgg 36000aaaatattgc aaggaggaat attatagttc agtaacataa taattcaaaa aatacatcaa 36060aatatatatt attaggcatt gacaaacaca ttttttctaa aaatctttag acattatatt 36120ccattttctt tgcatactat acgaacagat gcatgatatc attatcttgc tcatctgtca 36180gatactacat gcatggcccc aacttataaa ctaattcaac aataaagcat aaataagcag 36240tgccatgaaa tgagacttag acgaaagaag aaggagaaag aacgaacttg aatacctcga 36300ttccaccgat acagaaaagc aatatcagca gccagacaaa ccatgtggac atgaaaaagt 36360aaagcagcac cagaaatgtg gatgctgaga tgacaaatcc aatagcagtc catgcagtaa 36420tgtgcagaat ttcactatca tcctcctcct tgacggtcat atcatcatcc tgtaaggaga 36480gcatacactt tttaagacat tacagctatt aatcaagcaa ggcatcattg ggtgttatca 36540atatttgaca caagaactag aagtaggaaa accaagaaag acatgttcac aatacgctca 36600taacattcaa atacaaatca tagatataaa tagatcactc gttcaactat atttaaatca 36660gatcaactaa ggtcatatat gaatcttcag ttccattccc cgctctattc ggggatattt 36720cactccaata ctagataatt tgtattttaa cacaaacaag gagttccgca aaattagtgt 36780gttaaggtat gataagattt atattgatta gggtttattt aattttatta gcttgattag 36840ggattaaatc agattatatc tgatttcaat atagcaattt tcaaaattac acaccttgcg 36900acacttttga gaatattagg tcttctaaca ctacaccatt tctacccagt cgatatattg 36960tagctccacc aaccaaacag gccttcatta tgtgagtctt gtcaccacaa aattgtcgac 37020agtttttgag atcctccaaa ctgggaaaga cctctccaat ttcttctctg gataaatatg 37080tctctcacac acctttacct ttgccgttaa gggaaaagga tctatagaac aagccatctc 37140ctcgatgtca aaagtttttc catcaatctg gtggccaagt atacttagaa agcccattct 37200attccattcc ttttaaattc ttgacttact ggatccagtt tagcttcagc atacctcttt 37260tttcgctgga gatgctttgt aggtgacacc ctattagtgg tatggaaggg aaaaatgtaa 37320gcagtgcact ggtcaagaac taaagtatcc aaaggaagta tgtatcaagg ataaattatt 37380tggaaaaggg cagagtagtg aaaagattac acagacatat caaccttggt caattcagat 37440gtatgttaac ttctggggca gctcttgtga cgcaggcagc taagttttga aagaccaaca 37500actattacca attatgttac ctaaaatcca acagccagag taaactaaat ttactacatt 37560atcatcaaga tgcagtgctg aaaagctaag acaaatagcc caaacaaaat caatgttgac 37620aatgctacta gctgaattta aaagaatata agaagctgag catgtcaagt ttgagattgt 37680atcagacagc ttattttttt atttttttgt gtgtgtgtgg gggggggggg ggaggctctt 37740gtgatagttc atagttgtca ctttccttag attgagtaaa ctttttccaa agagctgcac 37800aaatgattgt tccaacagcc atcaaccata tgaaagacac cgagtagtcc acaatagggc 37860gatctggcga atataacagc agctccactg gattaagaaa aggattccat atgtcaaaag 37920tattccaagt gaaataaaac aaacaacaag aaataagaag atcagaacaa aagatatgca 37980tcaattagag agtcactata attgtttgca aataaaaagg ccatttgatg ttagccaaca 38040ctgcgtttaa gtccacattc tcatcagcta ccactgacat tttgaagaga aaatcaatct 38100ccgatcttca atcttcgttg catcacacta aaccttcatc ccaaactcat tgcttaactg 38160ttttttcaaa gtatcaacct gacatgcctt ttgcggtgaa gacacatcat cgagatccaa 38220ccataaatag atgaagaatc atctaaaagc tttctatgaa cacacaaatg gcatacggtt 38280cctacagaat acacccaata atatatgcaa caaggaccta ttgtttgcac caaaagttaa 38340ccagcaagaa gtgggattac atctcaatga tatataataa atctattttc atacttcaaa 38400agcactcatt aaaatgaatt ataatgcatg taatatagaa aaagtagatt acaacacaag 38460ctaatcaaca tcttgacaac caaaattaga atctaaaaga aagctaacct ttataaaaac 38520atctaagcaa tacatatgag gccagggctt acctttcttt cctgaattga tgtatttatc 38580aataatatct gccccctctt ttgaaattga aacaacagga atggttacat ttgatatggt 38640agaattatta ggacaagcaa tatccagagg acctaaataa atgcaagaaa aagcagcgtc 38700atatcagaaa gagatgaagg gaaacatata aaacagacct tgagacctaa gatgacaatg 38760tgagtcaggg ttacaacctc cttcattatt tattagcaca acacctcctg cacctccttc 38820ttgggcaacc gtggccttgg ttataaattc acattcaccg cgacgagcta gtgcaataga 38880gcctgataac tagaacaaat gatatttctg cattattaat gtataacaaa caacaccact 38940attattttct ttttatagga cattctcgag cctattttta caacagccaa acagatatag 39000caaatttaga cttctttggt attcaaagta aaatcagttt gtaatagtta acttctggta 39060gatcctagtg gtaacccttt cagatcattt tacaattcaa aattcctcca ccactactat 39120ttggttccac ttctgaaaag taccatgaaa aggatatttt tcctctaaaa taccagttgt 39180atttgtatcg ctaaaaataa cacaagatcg aagaaagaaa aaactaacag aattactaag 39240actcacaaat tgctaatata attgatattt ttgcaattgg ggtaatatat ggttattttt 39300agcaagaact taaaagtata gctgatcaca caaatagttt aaaataaaat cttgaaagtt 39360gaaaatgaag tcatgtagtc ttttgtaaat gaaacaaaaa aagaagaaaa agatctaaat 39420tttggaagat gatgtaaaca atgattatca cttgaaaaac ttataaacaa tttaagcaat 39480agcttatatg aagatgagtt gctgtacaga gaggggtgag ggtgttgaca agactcacga 39540cagaacaaaa ggttgttcag taagtattta actatttatc tagtatgtgt ttagttcatc 39600aaattaatca aaaagaagaa ggaaagattg tggctacttt tgagctatca agagatcatt 39660ccttcaccac cctgtacata gtgctacatc atcctcaacc tatctccatt tctactcttt 39720tctcctattt caaccctcca ctgcaatcca ccagaaaaaa gtatctttcc attttcacga 39780gtgaaactta ctcttaagtt ttcttatatg ctgaaaataa gttgttctat gcagagtaag 39840tttgaattac tttaacttcc tgatagaaga cagagatggt caatggctta acatgtagat 39900gttttcacct aaactatatt aaaaaaaaaa aattgtttct tcacttctcc ttgatataaa 39960tgagattggt tcacaattcg gctccttaaa tcttttcaac cttgctgaga tttgcatcaa 40020ttcttcactc ttaaagcagg acttggctca taaagaagta tgaattctat tgttttcaaa 40080tattcgcaag ttaacaaatg agagatggaa gatcatcaag aaagatcaaa agaaccgaaa 40140ttgggccacg aaagaattat gactattatt taatcaacca gcaacacagt tttgtttcgg 40200atatccacct aaaagtaaag gtgtgtaagg aatgtggagt aaatacctta gtggaggaag 40260cagaacagcc attcaaaggt tgtgtataaa cagcacgcaa cctggaggca cgtttagtat 40320cagtgggtaa tacagaccca aatgccgcac tcaagccaac tattgaatct tcttcaccac 40380cattaaccca caacttcacc agcatcttta acaaaagaaa ggaaaagatg attagcaccg 40440gtaagtttct atatgtgcag aacagaatga aaatagagaa tacttcaagt ccagttaaga 40500ctaaacatca actaacatgg atattgtggt aaaatactca tttcaatcat tgtttcttaa 40560agggcatgca aagtccaaaa tcgacaagta aaagtgaacg gagggagtat aataaatgct 40620aaataaatcc ttgtatagta atcttataac acacacacac acaccacaca ccagaacctc 40680aaattaaact tttttatgga gtggtgtctg agctaacttg cccacacctc aactattcca 40740ccgagtaact ctgccacccc ggcacagaat caaaacataa tatatcaaat aactccattt 40800cataacaaat tccaccaaat gcatgaaccc aatcctcacc atttcaaatc atcttattag 40860cattacccaa aaaagattgc ttttttaatg ataatcgcag tgtccaggcc agctcaacta 40920attccacgag atacgtacca cctcccacca acatgtccat accaaacaaa gattcttaac 40980tattgaaacc cctcaagaga aaacaacaaa aaaatccata accccatcat aactttccac 41040ataatcaaga aatgacaata tcaaataaga aaatctacaa aacccataaa agactacatt 41100tttaacacaa tttccaccca aaaaacaaac cccataattt attttctcta cacaagaaaa 41160gggttgaata catttaccat attgatttca ttgctgcaag agctatgtgc cttggtagga 41220gcagaatgtg ctattgatga tacatttaga agaaaaacaa aaatagatag tccaataaaa 41280cgccatgaaa atgccataat tatttattca atgttgattc ctcccttttt cttcctcaat 41340attttcccca attcttgcgc tatattttct tcgcctattt ctgcattgaa tttttttttt 41400tctttctttc aggggaatga aggaagaaag gaattttctt tccgatgaag aagtaaacaa 41460acttttaatg attaataaat ctcaaattaa attaattaat taaaaaattt aattatgaat 41520tggtaaaaaa tccaactcat attaatatag tgtgaaattt atgatttgtt gcatttataa 41580aaaaaattta aaaaaaagat aaatatgaat ttatttcatt ataaactttt atactgatga 41640gttaaaaata aagttaataa aaatgtaatt gtgtgtaata attacaaaaa ataaaagact 41700agaagatgaa aataagagac aggaaaagaa aaataatatt aaaagactaa tatatatttg 41760acaaacggaa aataaaagag caatataaat agctactaaa gtatttgatt acacaatgta 41820attttcacct ttccattcaa attaagatag tgtaattata ttgcaccctc tcaattgtat 41880tcaatatatc atattgatcc agtaattact tagctaaata tatacatatt aattatgtaa 41940ttacacataa gtttgatttt taggtgtttt tcaaaatatg attttagtaa atgtgatttt 42000cgtttctaaa atagagtaga agttcaggtt ttttatttag ttttttttca aaatcttttt 42060tttgggagca aactcttgtg gaggaaaaag aggcatttga ctagtaggat tcgcctaagt 42120gaattttgat tctctatgaa ttttttcaaa gaataaataa gagtcaatcc aaaattcaat 42180ttatgcctat gcttatcgat ctttcttgat ggatgtaaag ttatagtgaa ttctccaaat 42240tagagtcaga agttgagggg gaaccacact tccacctttc agttcaaagc tgcttttttc 42300tttatcatat atcgcattag aaagtgataa taagaatgac tttgacatac ttgtttatag 42360caagtaatct aactcgaagg ctctgcccct aaccttaaaa aatcgcatgt gcatgtctcc 42420cgtatacggt tcaagtggca aaagcatttt ctttgcgcta aatttgaact aatgatatgt 42480tagacaatct tttaccttaa taaacttatg agatatgcat atcacatttc tttcatgtgt 42540tttacatatt atttgaattt ccacaaaaaa aaacatattt tttaaagtca aaagcgcaca 42600gtttttttga aaatatctaa tatatgccct taattgtgga agagcgataa atttataaaa 42660gaaaaattca aaaagatacc aaaatctcac atattataca tagggtttca aatgtgatat 42720acaaattttt aatcttattt atcattaaac taaaatctgt ttctttttta tcaaaaattc 42780aagaaaatgt atgagtaaat taaattattt tttccataat ttcgcaatac aattttcctt 42840tgaaaaggat tcaattgaac tcatttgact ctctttagct ctattatatt tgtaatcact 42900agttttgatt cacattctga aaataaatat gttctttaat tagacattta caatatgaat 42960ataaaccaat aaaattattg aatattaaat attgaatagt taaaaataaa ataaaattta 43020gcaatacact taatcctaga tattctaaaa aaaacgtatt tattatactc ctagtgggat 43080accatactca aaccactaag atccaaatgg attatacttt taaaatggaa gatataattt 43140aaaaaatgca ctaaacatta tgtggaatcc tccccatggt atcaacaaag tgaggacaca 43200aggaagtcaa ctcatgataa tttatgtctt taaatttgtt tagttgactt tattttttga 43260tattcatatt gaagctttga ttaatttaaa ttcgtgctac ataaagttat ataaaggaaa 43320atattatcta actagatgtt ttttattatc taactagatg ttttttacat tttaatggaa 43380aaatcttatt gttaattaac ttgtcttgat attgtttaat tagaatgaag tgtttgttac 43440atgttaattt acaattagtg accgtgaaaa gaaaagatgt gaattgtcat tattattagt 43500agaattacaa aaagaataaa gactttttaa acattccttg tacaactcaa ataaaattat 43560tagtggaaaa tattgaagaa atattcaaat ttttgactag acaatataaa gtcaaaaggc 43620ttgttccata atgaaatctg aaattaattt aatttttcag catttccatt atatcattta 43680tttatttaag cactcaattt attaattgat attgaaagca aaaagtttaa tacacgttca 43740aattatggtt gttaagaact taagataact aactattata acaaatctct aaaaatatta 43800taagtatcta tttttcctat atgttgtgct ttgtttcaga ctctttaaaa atattatgaa 43860tatatttgtt tcagttcctt aacacatatt tatcgacatt gctaaagagt tcgatcacat 43920tgatttttac ctctaaaact tacctacaac aatattttgc ctaacaattt tatgtcttct 43980tttttgttaa atatttacaa tgttctctcg tggaaattta agtttttgtt ggacttaaat 44040aattatactg aaaaaaaata attccttaat tttcatggtc aatgaattgt ggccgctcac 44100ctttgttgat aaactatttt taaagagttt aattattttg atcataatca atgtataaag 44160tttatttatt atcatattaa gttctcttat agtatagtaa taaataaaaa attcaaataa 44220tcctagtatg ataatatgca tatgtaataa tacacaatat aatatgaaat aaagtaaatg 44280aatatgaaaa aattcattga atgagacaat gtcgactgct gagaaattga ttgttctgaa 44340aataataata atattgtgat gaaaatagtt gaacagtatg gagtagatta tatttcacca 44400tctatacaaa ttgaaacagt attattaaat aagaagggtc actaaaattt gagttgtaat 44460acacaaatta ttttaaaatt ttgtattata tggaaatatc ttatacctga atcttctcga 44520tgtcttcttc ccataattaa attatagaat ataatactac taaaataaag ctttgcgtga 44580taatggcaaa ttaaaaaaat aaaaaaatta atatcttaaa aatatttact atgtaacttt 44640taaggatatt atagcttgtt tggttaggat tttattagat ttgaagtgct tatttgtttt 44700agaacataaa taacttattt ttaaaaaggg ttagttcata tatttggtcc ctcaattatg 44760gatataatat atctcattaa tcattttttc cctttaaatc tcaattaaca taatataact 44820aatgaacact ttatagggca atttttattt tattatttta ttttttatca tataataaaa 44880gtgaatgtgc atattgtatt gtaagcataa tgtcatatga tactgtcatt gttgttgcca 44940ttacagagtc atgtctcaat aactgatttt gtagtttggc agactacata gctttctgat 45000ttttcttatc tatatttttc tattaaaata ttttttatat ttatttcata attgcaattc 45060aattttctta tacataattt actgcagaca tgcaccggtg gaatatgata tttaaaatat 45120tagttggtaa acttttaatc aagttgttgc tatcttacga caatatttga taacttagac 45180atatgaaagt gtatattaaa taataaatac atgatacatg tttactatat atatataaaa 45240ttagcactcg accgtagtta acttatgttc catgccaaaa acctaacaat tgtagcttta 45300gtttatctaa tcttgtttta tttataggtc gatataattc attattttgg ctcaaacctt 45360atatttttgt taaaaaatta tttaatatat tcaaaacaag caataaagat tatggtccgc 45420acttatacgg aaaaggtaaa agataaatgt catcttctcg taattcgtac ttgtattgaa 45480aaggtcaaat tatgataagc caatcaacat tacctaactc ttcttacttc agttcatcac 45540ttaatcatgg taaattaaca ttgttaactt aaatgatgtt ttacaaaagt aggggtgaag 45600ctactattat aattacgata aattcgacgc aaattcttta tattatttag gaaaactata 45660attcaaaatt gataaactaa aacctttgaa tatcttttta taaattgcat acagtcttgc 45720caaataagct aataaagctt ggtacaatgc caatatacaa tcaacaaaga atagaaagga 45780gaaaaagcac tatcccaaat atatataatc cacaaagtgt tgcactatac ccatgcacat 45840aaagtttatt caacttacaa atgcaatatg cactattgag tataataaat gaaaagtgca 45900aaactaagga tttgcttcta gaaactttac ccacaaaaag gcatgatgaa tggataatgc 45960actttaaact tttgaaacac ttgaacattg agaatgaaat aacaataaac taggataatt 46020tttttgttaa gtatttttat attaatatat aggtgagcgt tatattagtt aaataatagt 46080tataactagg ggtgtacaag tcaaattgta aagtcaaacc gaattgacga accaaattaa 46140accgaaaaaa aaactgatgt

gtgatttggt ttggttggtt tggcagttga aaaaaaaaaa 46200ccgaccactc ttgatttggt ttgatattaa aaaaaaagtc aaattgaact caaatcaaac 46260cgacaatata tatatatata tattatttgt tttatttata gataaaaata ttatttataa 46320tctactttgt taatatattt ttagttagtt tatagttttc aatgtttata tattttattt 46380caaattttgg gcatgtaaaa tttgtaaata tttattttgt attaatatct gaagttacaa 46440ttatattgtt atagtttttc aaattcgaag ttaacaattt actttgtaaa tattttattt 46500tgtattcagt ttgactataa cctttaatct tattaatgta acataatgaa cattgatatt 46560tcaaaaaaaa tattttgtat tcatgtgaat tttaccgtct tttcgaaatt tctattcatt 46620taacattttt taggtttagc ttataacacg ggtaagtgaa aatatatttg atctcttttt 46680caagcaccgt atagttgtaa aaaccgacta aagccaaaac caaaaaaacc aactttatgt 46740gttttgattt ggtttttaga tttaataagc cgacataatt ggtttatgtt ttttttaatg 46800gaaaatcaaa ccaaatcgac ttatgtacac ccctagttat aacaattcaa tggtagaaat 46860tcacaagaat gtatctagac atatattttg gtaagcgtca ctttggactt tctttggtaa 46920gctcaaaaga catgcatgta tacttcgctt ggagggggaa atgtacatat actcgatttt 46980aatgtcccta ttaaaattat attttgagtt tataaattct ttttcatatt tatcaattat 47040tatttcacga taactttaca tatatgatgt ctgaattttc atatggatga aattcagaca 47100tttttagtta gagtttttaa aaatattttt ctttcgtgat atttaattga aaattagttt 47160atttcgtaca aatccttctt cagaatttga agccaacctc atatttggaa gaaatgttaa 47220ataatgtata gtaatttttt tctttaaaat gatttcttac taaaattttc ttttaaagac 47280attttttcct aaattagtaa ttttctccca aatacatttg actttcaaaa gggcttttca 47340cacaaaggaa aaaagcattc atcatgatga cacaagcctg aaatcatcat atattaatat 47400ccatatacta aaatccaaat aataaattaa aataacaata aaaaaattag ctgtcttttt 47460caatattcaa cttacagagt tccctcaatt tgaaccccat tttcaatatt ctaaaaattt 47520tcatataaat agggaaacat agtttacaag atacacacaa aaataataat caagaattgt 47580tatatgagag ctatttctga aatattttta gtgaattttt ccccatttgt tagaaataat 47640tttttttcaa gaaagcctat tttttttcta ttagaaatat tactatgtct gaatcaagtc 47700aatatgattc ttgttttgat agaccaagat tggtgattaa gaaagtttta gctaaacctc 47760aaagtgaagg gaatggagct gttgttagaa gaagcattgg aaggtattaa aatttaggat 47820ttttatattt ttcacatttt ctttttgttg attttaagtt ttgtttctaa attttatgac 47880ttttttgtca tttgggtatt tttcattttt caatttggac aataaaagaa gtaactttaa 47940taggaaattg tgttttgaaa ctttggcctt gatgtgtttt cttgatttcc ttattggaaa 48000gtctctgtct taatgtgatt tctttctatt tgtcaagtct ggatgaatag agttacctag 48060tacctgttat tggtgggagt tgacaggtat ctagtgtaat tagttgaggt gcggaaaagc 48120tgccaagaaa ccacaattat caaaaagaaa aaaagtatct gtctttatga ttttttctat 48180agtcttgttg ctaattgaat gtggggtttt tgttttaaaa atttgatctt gcaggcatga 48240attgaggaat cttgatcctt tcctcatgtt ggatgaattt tcaggtactg ttttgagtaa 48300tgttgcttgg accctccaaa aatgttgttg cacatgtgtg agattctcca aaaaagcata 48360gcttttggag gatcaacaca cacgacaata tttttgaaga gtttgagcaa cataagtctt 48420gaacatctaa ctggaatgtg ttgattattt tatttttttg agccaagtga agttgatata 48480gtactgtttt attgtttcag tttctgctcc tgctggtttt cctgatcatc cacacagagg 48540ttttgagaca gtaacttaca tgttagaggt aaaaaaatta tgttgcctaa ttcaatccaa 48600tataacagta aggagaaagg gccgacgaag ggtggaaaat ggaaggccat ttcttgttcc 48660ctgaggccgt gtgtgtgcca tgaccaaaca cccatttgat gctctttaac ctccccatta 48720ggatggctcc gttgtattgg tatccgaatc atccaacaaa gtataagcag caaagcacca 48780aatggcattc cttgttgaat tgtactgagg cggatgtagt gtttacttga acccaatatt 48840tttgacacaa aacataaata aacgcacatc aaaatcttaa ctcatgattt caaaagtaaa 48900acgggcacag aagacgtgat aatgctaatg ttgtgttttc atttccttag ggagctttta 48960ctcatcaaga ttttgctggt cacaagggca caatcaatac tggtgatgtg caggtataga 49020atattcagct cttagcattt ttacttacaa gctatgttgc tcggatcctc caaaaatgca 49080taacttttgg aatatccgac acacgttcaa tagtattttt gaagaatccg agcagcatag 49140cttacaagaa cagaagagat attttggttg ctgatatatg tttcttattg tggatgatat 49200tgcagtggat gacagcagga agaggtataa ttcactcaga aatgcctgca ggagaaggca 49260gtcaaaaggg gttgcaactt tggataaatc tttcttctaa ggacaaaatg taagttatag 49320tcatttacat cattagaagc tttcagtctt atcactcctt ttgacctatg ttgctcgtac 49380tctttgaaaa tgctggcaca ctcgtttcag atcctccata aatgcgctat tatttggaga 49440atctgacacg cacctgttga catttttaaa gagtacgagc aacacaactt ttgacctgac 49500cccatataga tactttacac accaccactt tattgtactt ggctctagca tatagtagta 49560aaaaatttta atgttagctc ttatctactt tttttgtggg gcaggggggg tgttaggagt 49620gtactaatat gcagtataaa aatcacctct gtttctgctg tgttttgcta agcttaatca 49680ttcacttttg actaagttct caaaattttg gacattttat gatgtgatat tctagaaatc 49740aagatttagt ttgaaccaat attttcccat gttcaagttt atagcccaaa acgaagactt 49800tgtggcgaac acacgtttga tattggattg cttttccttt ttcaattctt attaagttca 49860aagttcaact gcaggattga gccaaggtat caagaactgc tgaaagaaga cataccaaga 49920gcagtgaaca atggtgttga agtaaaagtt atagcaggtg aagcgatggg tgtccaatcc 49980ccggtttaca cacgaacgcc tacaatgtac ctcgatttca ccctacaacc aacagcttac 50040tatcatcaag ccatccccga gtcttggaat gcgtttgtgt atatagttga aggagaagga 50100gtctttggaa ttccgaattc aggtcctgta tcagctcacc attgtttggt tttaggccct 50160ggagagggac ttagtgtatg gaacaagtct tcaaagccat taagatttgt tcttttaggt 50220ggacaacctc ttaatgagcc tgttgttcaa catggtccat ttgtgatgaa ctcacaagat 50280gaaattgatc aaacttttga ggattatcaa tattgcaaga atggttttga gaatgctaga 50340tactggaggt cagggcactg aagaaataga gctgaaacaa taatacaagc tatgttggtc 50400ggaatcttta aaagtgcata taggtgtgtg tgttggatcc ttcaaaagta gtgtattttt 50460tggaggattc gacattggtg tggcaacatt ttaggagagt acgagcaaca tagactatta 50520gcctcaaaat gcaccttttt tatgtttctt tttttctttt cataaatata tccctagtaa 50580agaaataatg gtcaacttta tcttgattta tcatttgaaa attgcttagg ttattgttat 50640ttattcttat tttcagtgtg gggtttaata gcatatttgg tcccttgatt atcaaaaaat 50700tccagttttc ttccttgtga tatctcactg acatttacca tacaattact ttgaaacgtg 50760cacgtttgat cactttgctt atgaatactc acaaaatttc aacacttatt aaccatatgt 50820taagttaagc ttgtataaaa acacacacat tctaattaat tgaagattaa atatgttgag 50880tcattctata tcacaagaat aaaaaatatt atttatcaat tattgaagga ttaatgctat 50940tctcccttca atgttgatag gtcgtgatca aaaagttgat tgttatttct ttttcttata 51000gtgaatattg attctgaatc tatgataata tcttcttgga tattgatata gtggtggtgg 51060tggggtaaga ttctttgttg ttagttggtt tctctctttc ttttcctgtt tcccttaggt 51120gttcagtaat tattttggag tttgattaat tcaaagtcat gttgaaagca tgcactattg 51180aatatgactt catatcctga gcttgaacct aagatctcta attaagatta tcggaatagt 51240tactgcttga tcacaacaag tttttattga caagttttaa atatgggaac ctctcataat 51300gttgctagaa ctaagagtgt tggtgaggat gaaaggaatc attttacata atttggtgag 51360aaaaggatgt ttgctcagtt tctagaagat gttgatatgc acagtttact gataagatga 51420ccttgacaga ctatactgtc tttgttcttt tagaatgttt tagttttgga gttgttgagt 51480tgcatcacta atgcatgtta taatcagctc tcccaaaaag caaacaattt cttttcgttt 51540ctcacccagt gtctaatacc tgtattaaag cccaactaaa acatttacat cagaaagtcc 51600catattgtga gtacactact ccctaacaaa ggtgattcca tacactcgag ttcaaaagct 51660ctgattgaat atgaaatagt atttatcact cactctacca caacatttgt tggtaaaaag 51720tggcaacaat tgaacaatca agaatgacaa atacataaag cagcacacag agttacattg 51780gtaaaagaaa catttttaat ttcagctgcc atatagaata taaatatcaa gttcctgacc 51840attacaaaag tactccatcc attaacaaat caataaacag taacaccaac aaaaataatt 51900taatgaacca ttcttgtaaa acttaagggg aatcaagaaa acataacatg aatcacctag 51960aacaacaaga gtagccctgc ttttgtttcg atccatcggt accatcrttg accaggttaa 52020ctctgttgac agataattct gctttataag aatcggaatt caaaaccttt ctactaacac 52080tattatagat ctctcgaatc acaatctcga aagccttgtt tacgtttgtt gaatcgaggg 52140cagatgtctc catgaagaac atcccttctg attctgccag gcttttgcct tcttctacgc 52200tcacagctct tatgttctcc aagtcacatt tgtttcccac gagcatcctt gctaccgttg 52260tatcagaatg agctgcatgt aaatggttgt ttcagttata gaacgattgg gagtatgaag 52320aacatataga acaccaccgt aacatgtaat ttgaacacct aatatacaac ggtataaata 52380aaagttggga ttctcgacag cagaaagtat cattccaaga aaaaatgtga ataaataagc 52440tcgtgaatgc aaagtttaag tacagcacct gcacatataa agagtctgta aaccttaata 52500ttactaccta tatttccttc aaaagtatgt atctgaattt tctcaagttg ttattagcaa 52560cgtcattgtc tcctttcatg aggaagaaaa atgctgcacc ttattaacac tgacttcgat 52620tttgatgaca taatcctctg ctcatttcat cccgtgatta aatgaagctt ctggagtaca 52680aacctcattt gtggttttat atgctgcaaa tgttaacatt ctatttatcg catttccctg 52740gccctgggct ctttaatgcc cttaactcaa gctaaaatct tctacttggg caaacggcct 52800tgtatctggc ccactcattt tgatagatca agagttaata ctcctctatt ttatggacat 52860taagttctaa aagttttcaa ctttggaaac agtgagcaca aagtcattca ttttcaagat 52920tcaaatgata tttgcaagac aagaacagat ttgaataaca aaataaggtc gtgcaagtat 52980cacatagcag ctcacggctc aaagcaacac atatacattc ccgtccagcc aaaagttagt 53040tagctaaggg cttaatagag agactaccta agttataaat atggcaccac ttaacatcaa 53100aagaatctca gagatttttt atcaatcccc gagactcctt agtctaccat aatgccatac 53160gtaggtaagc caggcagttg gtcctaatta ttttcacaat caagcaaata aaacagattc 53220catataagta tgatctgttg acaagttcat ttagattggc ttttcggaga aagaaaagca 53280tgacttgatt tccaattcct aatatacatc caattgacag tctgtctaat tcgttcttat 53340cataggttcc caactgcatc tagacaaaat tgagcataac agctacattg gcatatatac 53400cgctttcttg gccattgctt caagtaacaa tgatccataa tcaagactca aggtttttct 53460agctaacatt accatttgtt tttatgcctg gtaagtagtt tttcactaaa aagattgaat 53520tagacttgat agcaactgta aaatcttact ttattcagtc aaagagaaaa gtgataggaa 53580ccaaatccac agaagaagaa aaaaagcaaa ggacaagatt caaaaaatat aaattcaaca 53640cattcattat tcaattctgg aaaaactcaa ttaaaattac ccttatggct tattccatca 53700tcctaaatag ttaaagtagc caagactttt cggatctaca agatatctta catacttata 53760agttataatc aagaaccaag aatgtggaag tcaaattcca gaatcgccat catatcataa 53820caccatcaaa tctcaaaaac tgaacttcat actaccaacc tcaatatatc tcaaaaaggt 53880gagaaccttt gtattaaact ttgatgggaa ctcaacatat gccaaaacca agttgaaaag 53940tgggaattca acatgaacca aaaccaagtt taaatttttt tgtagcaaca gagacatatg 54000aatacaacaa tcagatctcg actcacttgt caatttatcc aataactaat tataaacaaa 54060atgaataatg tagaagtcaa atcaaaagaa ctaccattct atcataacac catcaattct 54120aaaaaaacta gactttacag ccaatccacc aaaaaggtga gaactttctg tgcacaaaga 54180aaagataatg cataaagctt agatgacaat tcaacataaa ccaaaaccaa gtctagaaaa 54240agagaagaat tttagcaaca gatgccaaat ctaagaatta ccattatatc atagcacaat 54300caaaatctca agaactaaac tttatagcca atccaccaaa acctcactat atttcaaaaa 54360ggtgaaaact ttttcttgca caaagaaaaa gatatgcata aaactttgat gggaattcaa 54420cataaaccaa aaccaagcta aacaaagaaa agactactag caacataaac aagaatgtag 54480aagtcaaatc taagaattac cattctatcc taacaccatc aaatctaaaa aaaactaaac 54540tttacagcca atccaccaaa aaggtgagaa atttctatac acaaaccaaa gacaatgcat 54600aaaacttgga tgggaattca acataaacca aaaccaagct aaacaaaaga attttttcag 54660caacataaac aagaatgtag aattcaaatc taagaattac tattccatca taacatcatc 54720aaatctcaaa agactaaact caagccaatc caccaaaaaa ggtgagaact ttcaatgcac 54780aaaccaaaac taatataaga attcaacata aaccaaaacc aacttataaa aaagagaact 54840tttagcaaaa aagatacata cttttgagtt catcaagcca acgagggatg ctatcaaaag 54900ttgtccttct tgaaatatca taaacaacaa gagcaccaaa agcaccacga taataagcag 54960aagtcacagc tctaaacctt tcttgtccag cagtatccca aatctgagct ttaacttctt 55020ttccatcaat ttcaagagtt tgggtctgaa actcaactcc aatagttgct tttgaatgca 55080aattaaactc atttcttgca taacgtgtaa gcaaattaga tttcccaact gcagaatcac 55140caatgattac aatcttgaaa aggtactcct caccctcatc atctgaagag tccatttggt 55200gaaaaattga aactttttta tgtttagaga gagaaagaat tggaaaatgg tgatctagag 55260agagaggggg aaaggagtgt tggggttttg tgcaaagaag taatggtggc agagctggca 55320aagattatac agagaatttg aaagaagtgg tcttgctgga tttttatttt ttgttatttt 55380gttttttatt gtttttttaa taaaataaaa tcataagtgg gtgttctttt ttaggtaaat 55440aaaaaggaat atataataat tatataacct tttgtttgaa aagaaaacag gcggttgaag 55500acttggagtc taatagtttt tttattaaaa aaataacttt ttatgataac cttttgcctt 55560tttccttctt tttattggtg taatccttgt gatatatatg atttaacata tttaaattat 55620tcaatataag aaattatgtg ttcatagttt ttttaggtaa gaaatatgtt aactttgagc 55680tatttttaga agtacattat gtactcacaa attaaaaaaa tttcgttttg actataaatt 55740ttaggagtat tcacttttat ttacaaaata catatctaaa catatcttta catttcaaac 55800agtactattt ttcaacataa cttcaaaagt tttttttttt ctttcaattt atggctaaac 55860gttagcaaag ttacaccaca agcttagtat aatacttggg ttattaaata tgatagaaca 55920attaatagtt ctaaagattt gaatgaagaa aagagaaaaa aaaatggcat gtttcaagta 55980attacaagtt aaattatata tattatcact ctcaaaaatt aaaattcgaa tatatcaata 56040attgagagat caaaattgtt agtattggct atttcttttt ttaggggaac aataaaagtt 56100tctcacattt gcatagaaaa agactccata tgatgtgaga aagacttaac ttcgaattgc 56160cagtggggtg ggggtggggt gttgtacaag aagtgatggc aaagctggca aagataggta 56220cagataattt tgttaagaag tggtgttgct ggattttatt aattttcctt ttctaggatg 56280attttataaa tggaagaata atagtcaatt tgttctattg tcttaagcag aatatttttg 56340caagactaat ggtaaaagta cacaccatcc tgcttttttt ggataattaa acggtaaatt 56400gtttaattct tatgtgaatt tgaacatcaa tttctcttta taaaaaaatt caagctaata 56460taaagtgcat gaaattagtt cttgagtcgg ttatgattaa aactcatgga ggaccacatt 56520gactcaataa ttgagaaatg attgtaaaaa atcacatgga ccatgttttt catggacatc 56580cactccctat aatatgataa tattgtcatt acattttcaa tgaatgatcc aaacaaaaaa 56640ataagatatt gttcaagaag tgaatatatt tttccactat ggctacaaga attaccatca 56700attttgaaga aataaaggat ttaaatacca cttcatccaa cacactacat ttgttgaaag 56760gaaaaaaagg actattaaga gaacttatgt tgctcgaact attaaaaata tcaacgggtg 56820caagtcagat cttccatgtg cattttttaa agaagcttat acgagatacg acggtctttt 56880tggatagtcc tgacatgaaa attgaatctt gatccaaaca aaagagacaa gatataattc 56940aagaattcaa cataaattac aaccaataca atcttgaaca aatgaaggaa atgaacaact 57000aatataactt caatcaacac acatcacatt tcaaatgaaa acaaagacta aaacaacttg 57060aaatcataac atttataaga aaactaatga atattgtact agtttatagg ctaattttga 57120tgaagcatat aagatgtaac tctgtttact catttagaac accattgcct tgacctttca 57180aatccattct ttccaagttg ataatcttga taagcctgca taatctcact cttagtgttc 57240ataacaaatg gaccatactg cacgacaggc tcgttaatcg gttgccctcc tatcagaaca 57300aacctcaatg gttttgaaga tttgttccac acacttaggc cttcaccagg gcctaaaacc 57360aagcaatggt gagctggtgt agtagttgaa tccgaagaac caaacgcctc ttctccttca 57420actatgtaga tgaaggcatt ccaggtctcg gggatctgtt ggtgatggtc agaacctggt 57480tttagggtga agtcaaggta cattgtaggc gttcgtgtga aaacttggga ttttatgccc 57540atggattccc ctgctaaaat agtaaccgaa acgccatctt tttccacttt tggtatatct 57600tggtgtagca attcttgata ccttggctca atcctgcatc atggacggat aaaaggaggg 57660aagttacaaa aacggggcaa tgaaggctcg tgaagtagac aacaagcagc aagacaagga 57720tcattagttg ttgtgtctat tgcatggtgt agcctcacac attgaaggct ggttagttga 57780acaccctttg tcgactcgta gagtcaaacc atactttaat acatatatat tagatcttaa 57840acgcctaaat tttatgaatt ttgatcaaag tacatgtaac aggaatatgt tctaaaatga 57900aatagattgt tcaaacacta tattttttta agccaaagtc aagattgaat caatgtaact 57960tacattttgt ccttggaaga tagatttatc caaagttgca accccttatg agtacctggt 58020ccagcaggca tttctgaatg aactatacct cttcctgctg tcatccactg cattaaacca 58080aatggaaaat cataaacaca cttaaatttc ataaattcga tatccggtcg catcatgaag 58140tccactttag gaggtaacac actccttttc aaatgtgact acatttacaa gccttgaact 58200cgagacctct aattaaggac gaaataatac ttactattct accaccatga attttgatgg 58260taaacattaa ttggttacat tagtaaaatg agtagctaac aatctatcac atgttatata 58320acgctgatac tattaaaaat ctttacactg tcagtgcaca taacttaaat acttaagagg 58380tatatatggt ggtgttgtta gcaaacacat tacctgcaca tcaccagtcc tgattgtacc 58440cttgcgacca gcaaaatctt gatgagtaaa agctccctaa aattaaacac cacaaaatta 58500cattagaatt ttcaatctcc ataatccata caccaacaca gagtttaact tatatacatc 58560accaatacct gatagtatat atcaattttt tttgtcgatg tacaaaagtt aaactttcta 58620cccaaaaaaa ggaaaataaa atgaattttt acctgcaaca tataagtgac ggtctcaaaa 58680cctctgtgtg gatggtcagg aaatccagca ggtggtgaaa ctgcaagaag ccaaaaataa 58740catagctttc taattagtca aaaataaaaa taaaattcac aaaataatat actaaaagaa 58800taaaataaaa gaaaaaatta ccattaaact catctaacat gaggaatgga tcaagattct 58860gcaattcagg cctgatcaaa ttccaaattt cataacaaat atatataaaa aaaaaacaat 58920cagtattcga ttcgtattgc tgacaaaagt atgatattta attggagaag agtaaaaacg 58980tcgttagaga tttctcgatt atcaaaacat aaaaaatcaa gaaaaaggaa ggaagtaccc 59040aaaaataatt accttccaat gcttcttcta acaatagcac catcaccttc attttgagct 59100ctagccaaaa ctttcttaac aaccaatctt ggtctactaa aaatagagga catagacatt 59160ttgatgtttt taatgtttgt ttcagctaaa tttatttttt gtttgttgtg ttttcttttg 59220attgtgctaa tgtggctatt tataggcaat ttattcttga ggattcatat tcattgaata 59280ttgatgacga aatatattac atattatttt cgtgtgttga atagaagttt caagtggggc 59340ctacttaatt tttttttttg atgatttcat gctgatgtta ttatgacgtg gtttcttatt 59400aaaaaaatta tttggataaa aaatgaggaa tatactatcg tatgaatttg tagagttaat 59460tgactaaaaa gaaaaataag ttaaaagaat aaataagata aatactagaa ttattagata 59520ttaaagaatt caaaataata ttaaatatgc atgaattata gattgaataa ctaaaatgga 59580aaataagtta aaagaataaa taagatacta gaattattgg atattaaaga gttcaaagaa 59640tactaaaact atcatactta ggatttgaac ttttgacgta aaataatctt ttgactcatt 59700ttgttgtaat actaaaagta tcttttgata taaaaaaaga ttctaccatt tatatgtaat 59760cgaagcatat attatggagt agttttttta atgaggaatt caataatata tacctagttc 59820ctctaatttc atgagttaac tcttaaatat ttaaggattc aaattaatac taaaaatgtc 59880atacttatga tttgaagttt tgacgtaaaa atctcataaa aggtagaagg taaattttaa 59940gtaccattta tacgtaacaa aatgaaactt cttttggcaa gaaattcaat tatatatctt 60000gccaactacc tttcctttgt gatttcacaa gttaccaata aaaattgtga ttgaatcaat 60060agaatttcct cattttaaaa agttttaaat ttaaagtcaa tatatatata aaagaattac 60120attaaaaatt aaattgtttt ccttaaataa ctttacgtga tataaattca aaaattaatt 60180aaataaaatt ttatataaat atgaaacatt aggtggagga ggatgttact tttgaattga 60240atgtttatac ataattattt aagaaagaaa catctagcaa cttcaaaaat caaaagagaa 60300acaatgtttg gtagttgtcg gcatgtaaac tttgtttatt tttgctgcct ttgattcgtt 60360gaccgttgac gtgaccaata tctattcaaa aattataaaa ataataatat tataaatttt 60420tattttttta tatatgataa aaaaatatac atcgtaaaac gttaataaaa gtttttatca 60480tttgtctcta aaatagaaaa ccataacaac taaaagaaac gaaaaaataa tattgaataa 60540tatacaattt atttattttt attgatgtac aaaaattaaa atgttcactg atccaaaaaa 60600gattcaaaaa aaattgtatt tgatttagta ataataagac aaatcatttg ttcataaata 60660ctttatactc atcccgtgtt agaagcaaaa cttaattcat gaacttaaga atcacaattt 60720atgttttaca tattattttc attgtatttg atttacaaga tattttataa cttttatttt 60780atagataaaa atttattaaa aattttagaa caacttatgt ccgtggacaa tatttaacac 60840catgtccttg ttctaattta taattctctt tcagtttgca aatcttaact ttaactaaat 60900ttaaattttt aatcgataga aaatactagt cataaaagga taaaaatatc tttacccata 60960catatagatg tataatgaag ataagtgtta aaagagattt tttgattgaa ataattcatt 61020atttaatatt ttattgtaaa aaaagtcact aaaaataagt acgaaaataa ctttaataaa 61080aacaatttga agaaaatttt cattatttgt taaccttcat gccctatcaa acaatacaaa 61140aagactatgt tttcttttaa tttttcattt ttttacacat ctaatataaa catcctttca 61200atagcgtata gacattattt

cattcttcaa caaataaaca ctatcaacta acaaataaac 61260gtataaaacg ttaccagtaa taacaaattc aaaactcatt actctcatct cgactttaat 61320gtaagttaaa taaatttaag atcagactct aaaaccgtta caattccaaa aaaaactaaa 61380aaatgataaa atatccacaa aatacttatg gtgggtccca acttaaaaac ttttaattac 61440ttatgtccca ctagcctttg acttaaaaaa atctcaaata attgaatcaa caatcccaaa 61500aaaatcccaa aaaattctct aaaccaaata tttcaatggc gaaaaaagca gtgttaattg 61560gaatcaatta tccaggaaca aaagcagaac ttagaggttg tattaacgat gttaatcgaa 61620tgtacaattg tttacttaat cgtttcggat ttgctgagga agacattact gttcttatag 61680atactgatga ttcttacaca caaccaacag gacggaatat acgtaaagct ttatcggatc 61740ttgttggatc tgctgaatca ggggattgtt tgtttgtgca ttatagtgga catgggacta 61800ggttacctgc tgaaacgggt gaagaagatg atactggttt tgacgagtgt attgttcctt 61860gtgatatgaa tcttattaca ggtaattcta catttttttt atttaaagca ctttttttta 61920aggatttgga gtaggggaaa tggggcgggg attatgaagt ggggaattgg aaattcaggt 61980agctggtgag ctactgagat tgtacttgat ctatatgatg ggtttgttcc aagattgttt 62040tttttttctt tgtgtttgtt gaatttgttg gggagttagt tggtttgatc taaatgatgg 62100gtttgttcca aaaattgttt ttttttgtgt ggtatgcatt ttaatgcagc tatttgagca 62160tttctttgat caaaattagg aattgagtag tcaggtagct aatcaactga gctagtaaga 62220gtgtacttga tctaaatcat gagtttgttc ccaaaaaaat gttgtttttt tttgtgtgtg 62280tgttgaattt attgggggtt aagttgtatt gctttgatct aaatcatggg tttgttccaa 62340aatttggttt tttgtgttgt gggttgaatt ttataggtag ttacttgtat tgctttgatc 62400taaatcatgg atttgctcca agatttgctt ttttggtgtt ctgtgttgag tactacttgt 62460atgctttggt ctatagatca tgggtgtctt ccaaaatttg tttttttttt tgtgttgtgt 62520catgtgtgtg tgatatgagt tttattgcag gtacttctat tgctttgatc taaattatgg 62580atttgctcca aattttgctt tttgtttgtt ttggtttggg ggttgggggg tagagttgat 62640gtggtaaggg atctaggaga ttgagatctt gaaatggttt tcttaaaaaa tgatcttgaa 62700aggttgagtt gatctgtttt gatgataatt caggtggatt gaagatataa ggagatttgt 62760aaaacgagga aatgggattt tagaaatgag aatttgggtt tctagagtaa ttcaaatagt 62820tgaatggggg acgaggaaca gttggaaaaa aagagggagt atagtttaca gcattgtgat 62880atgaatctca tttacaggaa cttaactatt tccttgagct taatcatggg ttgttccaaa 62940atttgttttt tttcttcgtg tgtgtgattt gtagattggt atgagattga gatcatcata 63000tagaatattg tgtaaaaaag gatcttgaaa gtgcttagtt gatctgtttt gatgattatt 63060gagacggatc ccgagtttta gggagatttg tgaaatgggg aaccaggatt tagaaatgag 63120aatttggaac cagagctatt aaaattgttg aatgggagat cagaaaaagt tggcaaaaaa 63180gaggagaata gtcatgatcc cacaagtggg gtcttgaaag ggtaggatgt acgcaaacct 63240tacccttgat ttccgacact cggctataga atttattacc tcttggtttt ataatattgg 63300aaaagtaaca ggcaatttaa tattgaactg ttggacgtgt tgtttcatcc ttgatcagat 63360gatgatttta gagagctcgt tgacaaagtt cctgaaggtt gccggatcac aattgtatct 63420gactcatgcc acagtggagg cctcattgac aaagctaaag agcagatagg ggagagccac 63480aagcaaggtg atgacgatga aggccatgga tctggttttg gattcaagaa tttcttacgt 63540cgaaatgttg aagatgcatt tgaatcccgg ggtatccaca ttccacgtcg ccaccatcgc 63600cgtgaggaag aagaggagaa ctttgctgag agtagtgtga ttgagacgga agacggtgat 63660caagttcatg tgaagagcaa gtccttacct ctttccactc tcatcgagat acttaagcag 63720aaaaccggta aggatgacat tgatgttggg aaacttaggc caacactctt tgacgtcttt 63780ggtgaagatg caagtcctaa ggtgaagaag ttcatgaagg tcatttttaa caagctacaa 63840aagaataacg agcagggtgg aggtggtggg ttcatgggta tggttggtaa cttggctcaa 63900gagtttctga aacagaaact tgatgaaaat gatgagagct atgcgaaacc ggccatggaa 63960acacacgttg aaggtaagca ggaagtttat gccggttcag gtaatagagg tcttccagac 64020agtggcattc tcgtcagtgg gtgccaaaca gaccaaacat ctgcagatgc cacccctgca 64080ggcggagatt cttatggtgc tctaagcaac gcaattcagg aaatcctagc tgaatcagat 64140ggtccagtca ccaatgagga acttgttagc aaggctagga aaaagatgca gaaacaaggc 64200ttcacacaac gtccaggcct ctactgcgat gatcatcacg ttgatgctcc ttttgtttgt 64260tgattctgct gtgctatgcc ttctcgagta gctgttgaaa atacgactgt gaagttaacg 64320aggtgttata taactacatc cttttgtgta tttgtgtgat tgtttgtgct tatgttggag 64380tagctcttct actctggtgt tgcagaagaa ccattctcca tcatgtgaat gtgtatttgg 64440ctctgttttt ttttcctttt aaatttggtc agagaataag gtattaaaga accacagttt 64500tcggtattgt tttggatatt tgaaagttta atctggagaa cttgtcttgt atactgaata 64560tactgaatgt gttataattt aatgcaactg aatgtataaa tttcttatca tgtgccatca 64620taagaattga cctttgtctt gtgaaactag ggaacagaac gaaaggagga gctgaatttc 64680aaaattttgg tttacataag aaaatattca cctgaattgc actttcatat tacatcttgg 64740atatatatag taatcaagta caacaaattc ttgcacgcat aacacaatat cgcaatgcat 64800gtaaacaata ttactcgcgc tagaactcag atatggttat ttaaatttca aagagatttc 64860actcattcga taacagtcat tcttcaaatt ttacttgtac aagtttcaaa gatatatatc 64920aataactcca gttgtgtcca tgactccatt ccaaagcttt atccacttgg aaaagtagaa 64980gtaccaaact aaagcaacaa aattagctaa gccgcgatct acttatgttc cttctttggt 65040tcaatatcac ttggatttgg gactggttga attctttggt aaggtttcct tgtcaatgtt 65100tcacccttca gagctgcaac atatctgtca tttgtgttct cctttctctg cagttcgcca 65160aggtactcag ccaacctctc tttattaact ttacccatca tctgttacaa aaatcaaaac 65220aatgtatgtt taggaattca acaatcgtaa gtcaaagtac taagctataa aagaaagcca 65280atgaaaccag aagaaaccac tgtgagtcca ttttttttat ctactgtaaa gctttatcta 65340cttagctctt cataaaaaaa agcaaagtcg atgttctttc aaacagcaaa tgactgaaca 65400agatagagaa gtgaacatta tagctatttg tagttcccat agacagcagg tggccgattt 65460catcaaaggt gtaaaccaaa gttacgtgga agtgctcaca actctgtttt agtttttaac 65520aaagacaaga cataaatggg tatattcaaa tgctgtgttc atcttcacct acaatatatc 65580attcaaaacc aaactagaaa aagagaaggg ccatttgtaa tatgaaagaa aagggtctga 65640aattaagctt tattctcact aaaatagtcc tgctcggcag tcaagccact tcactcaata 65700gaggttcttt ggggacttaa agaacttagt tgtccttcct ttccaattta caagttccaa 65760aaatacaagc tggaattgtt atccttatct atctatttgc cttctcaaca attctctgct 65820cttgccaaca ttccgatgct ccatttactc accctggcat ggtccaagca cccaaaggca 65880agccaaggta gatagtgatg gattcttcca ccttacggcc caaaatgctg gtagaatctg 65940tacgttaaaa ggtaataaca ttcgagtcgt ttacagctta acatgcacaa gtcaatatat 66000ttaacccatg ctctcaagta gctttagcaa gaaaggccag ctaacatggt catatgcctt 66060gaagtctaat ttgcacattg ccccagcatc agtaaacttt atcttggact ccaactctct 66120aagctcatat tagctactag cactcccaca aagaaaaagg attttgtgat ttagatacca 66180atttgtctat tattgccctc aaccttccat tgaggatttt ggcaataatc ttgtaactat 66240tgccaaatag aatagaggtc aaaagactct aatcaacacc ctttcctctt cagggattat 66300agcaataaaa atcacattta ggcttctatc tagtttctcc accttaagaa aatacttgaa 66360gctggtcttt gaatcttcct taatgattgg tcaagaaaaa aatgataggt caacatattc 66420aacaaaaagc ccttggtgaa actattgaat tccagagact tcgtttcgta cactgcttag 66480atagcaagcc atacattttc tcagtgaaag gtctttcaac tcagtcctct tctgctccac 66540ttataatgta ttccccagtt cctcaacata ccattcatgc cttcacttct ttgtctctct 66600gaacaaattc ttgtagaatt tcaggatcaa cattttaata tttgttttga ctacaactta 66660gaaaatacac ctgacaatct aatatcttaa actccctgat ctgtcatatg gtgtctcata 66720aaacatacag aggaacctat caataaataa ataaatatag aggaggtgtt ccaaccaaat 66780tctcagaagt aattcaattc ctttacactc ttcgttctct atctaccaag actagctatc 66840gattgctatt ttctataagt taaatatcaa tcactttatc tacactaaaa aataaagctc 66900caaacatcat tttcttttta aaggtaacaa gtacatcaaa caacaagaaa cattatcaaa 66960tgatactgcc aggttacctt tcattttcat agatcaaaaa gcacaatcat cacagcagaa 67020tgtacaatag acatgaggtg catactgatc tcgccatatg aaagtttgct ccagatatac 67080cattactatt aacagagcat ctttcaaagg cctttgctta gctaactgtt atgaaagatg 67140ttctggtaat aacagtcagc taggcaaagg cgaaagggag tacaatacag aataagaacc 67200aacaacacaa atcaatatgc gtacaatcat aacagcagaa gctacaacac atatgaatca 67260ataagcatct ggtaaaaaca attctactag gtgaggggac tctaaaacac attagaaaat 67320gataacaaca aggcaattct tggtataatc atcacagcag aatgtacaac acatgtgaaa 67380cagatacact catcctacca gattgaagat gctcctgcaa taacactcag ctaagtagca 67440gccaaaggaa gtcaaagcaa agaatgattt ttctttcctt cgaggctcca gggtaaagat 67500acatttttta cgaaaaattg ttaaaaaaat tcagtttatg ttagcaaaaa ccatgttccc 67560ttctttttct gatattttta taatggatta gtttaatgaa taacaacgaa tcaactgatt 67620gatggtctat ctcttttaga ctatagttaa tggatattct taaatcataa ttgtaattat 67680atagactatg attccatacc agaaattttt taaacaatac acaataagaa ccaataatac 67740caagtcaatt tacgtataat cataactgta gaagctacat cacatacgaa tcagttattc 67800atctggtaat aacaatacta ctagaaaaga agactctaat acacattagg aaatattaat 67860gacagcagaa tttccatcca atcattaaac cacaatatac aacacatata aagcagatac 67920tcatccgtcc ataacagtta gctaggcaaa aacaaaggcc aaacggagta caacacacaa 67980taagccaagt aaatttacat acaaccaaaa ctgcagaagc tacaacactt atgaatcaga 68040tactcatctg gtaataacaa tactacttgg tgaagggact ctatgttaat atcaaagcaa 68100tttcacgtag cagaagctac aatacttatg aatcagatac ttatttggca attacaatac 68160tattaggcga ttgataatcc taagcaattt cccgtataaa acagcagaag gtacaacata 68220catgagcaca aactgatcta gttttagcaa tatgagtatt cgaaggggaa gtgaacatac 68280tagggtttcg gggcgagcgt tcttccggag tttggcttca agttcaggac tgcgagtatt 68340ggtggctgac ataacgcaga aaccaataat ccccggaatg acgccgcaaa cggtggcgaa 68400tccgatgaag aacggcttcg attccttcgt tctcctcact aaccaccgcc atgttccact 68460cttctcccac agaatcgaca tcgacttttc tgatgattct ctcaacactt ttcttctgct 68520attttctaga gtagaacagt gcaagggaga aataaaaatg acaaaaatgg ctccttatat 68580ttgcggaaca ttcaaaatag tccttaagta tgaattgatt gacaaaagtt aagatccaag 68640tatatttagt aatttatatt ttatatttat aaataaattt gaaggaaata atgcaaaaaa 68700gaaagatttt tgtaatttta aacatcaaga aatctttaaa aaaacaataa aaagaacaaa 68760ctatatagag taaaaaatat caaaaattgc actttaaaaa aggtatataa ttttgatttc 68820gatctatctt aagtggacta gaattgatat cttaataccg attctttggt ccaaagcgga 68880cagctcagga gtgaaatgat tgtgtgtatt agttgaacca cgaaccaact acccatagaa 68940gaaaattatg ttctaaaaag gatggttttt ttttaataaa aagagaattc tttgaactag 69000tttgcctaca attttacact catttgttag tacatttcat tgttagtgaa tctcctatat 69060ggctttatac caaaggaaac agagaagaat ctgtccaaac tttggaaaat attgcatctc 69120cagcaacaag aagcagtaaa tttaaccttg agctttttcg gatcgttcat cgaattagaa 69180aatcaagaac tactcaacaa caacaacaac atggttgatg tgtattgagc tatcaagatg 69240ttaatggaga gaaattggtc aaaagtataa agctgcataa aaacaagaaa tcagtgacag 69300accaactgat atatatttgt ctccaatcat acataaaaag agtttatcaa tgaaaacaaa 69360catagaaaat agtagtagtg ttacaataaa agagaaatgg gctagacttc agtatgagca 69420tggctgcaac aattagctat taaaacctca ctaatctgac tattctttga cgaggtgtat 69480acgattacat ccttcaatct cttcgatctc ttccttaatt ctcacagctg aatcctccag 69540agattctttc caggacccaa tcaatttaat ctgtttcaat gatggattat cagcaaagct 69600aagtgggatc tcctctaggt caccacactt ttttataaca agtgtttcaa gcaagggaaa 69660tgattcctcc gaggcatccc accttgagat acctaactgc accaatttca acaacttaag 69720tttatgaaac ctgatatctc caaggcacca ctcttctgat tgaggaaaac acacgtcctg 69780taattggaga tactccaggc ttggtagttc cgcaatgaag gaaatagcac tttcaatatg 69840aattccttta agtaccagct tgtttaaatt tgaaggcacc tgtaacccgg atagaatgcg 69900gggatgcaca aaggaaagtt gaagttcttg aagctgggta agattctcca atttgggaca 69960aaaaggctct gcagaatctt catcatcctc aagtgtgatt tgaagttgtt gaagattagg 70020acacctcttt gataacacat ccagactatc aatcggaaat ccaacaattt tctttaatat 70080cctcaaattt tctaatttag aggatccttc aaagagcccc tgcttatcaa aaactgccca 70140accaaaatga gcatgcctta atttttccat ttccaaaaaa gacactggta accgtacacc 70200ataaggaaaa ttcctcacaa ttaaagtttc tatatggggc agatgtgttc ctggatcaaa 70260atagaattta tctgcccaaa ctgcgaggta cttcagctga tttagtggtt tgaatgtagc 70320aaacgacaaa aactccacag tataagaaat caaatccaag actttaagaa gtcgcaattc 70380agtaatctga cagaagggaa tcacatcaat agattctcca ccattagccg ttatcagaga 70440cctcaagtgt tggtggaaag gcttctgtgt tttggaaacc agagatgcaa acttggaaag 70500ctcttcactg aaactgaagc tcactcgact tcccttccaa tccaacggtt gaaactggat 70560atattgaccc ttcactgcaa gcataaactt ttcttctcta ctcttcttca agcaaaagtg 70620atgcacaaca tcatgaacct ggcagcattt gactttacca ttataacctt tctttgaaac 70680aattaccacg ttactgctaa tgagatccat caagtaacct tcagcttcct cttccattaa 70740tctcccagat tcagtgttct ccacgaatcc ttcagcaatc cataaactta tcaatttaga 70800tgctggaatt cttgcgtcct ccgaaaacat ccccatataa agaagacaag gctttaaaca 70860atcgggtaag ttatcaaaac tcaactgcat agtcgccaga ctatattctt cgaactcact 70920gtcaagatag tcaaataaag catctttcac ctcattccac caagattctt ccattttcct 70980ttttttgatt attccagcta ccaagacaac cactaggggg agtcctttgc atttttctgc 71040aactgcttga ctcacatatt gtagttcagg cgggcaatct tccttttgaa acactttttt 71100ctgcaacaat tggcaactct cttctgttgt gaggaatgga agagaataag gatcagtatg 71160gtacttgact tgcttaccca cttcttcaag tcgagttgtt acgactattc tgcttctcat 71220tccaacatct ggaaaagaaa gccttaagtc atcccatgcc atacaatccc acatatcatc 71280caatacaatg agatatctct ttcccattaa ttttctcctc aacatgtcgg caagaacgtc 71340aaccgtatct tccttgtgtt tggaacctgt aacttgacta aaaatatctt gtaatagctc 71400tctccgatta tatgtttgag aaatgatgca ccatgctcga acatcaaagc gagaaacaat 71460gatgtcatta ttgtacagct ttctagcaat tgtcgttttc ccttgtcccc ccatgcctac 71520aattgggacg acgtctagct catttgtacc tctaatcaga taatcaatta gttcttctgc 71580tttattctca aaaccgacta cctcctcatc agtcacagga ttgctatgtc gagctggcag 71640atgtttaaat ggagcagcca cgtagtgagg attaaaagga atgtccgctg accacatctc 71700agtcacctct gcattaattt gcttgatctc ttttaaaatt gtaggaagtg agcaaaaaat 71760atgcaaaaaa gcattatact gagcaagaat agaatcaacg gcaacctcag cctcatatgc 71820caaattgatg gtacgtctct gaagatcttt aggaatttta tgttcgtggt gcacgatatc 71880tctgaaaatg gatgatagct ccttctctaa aatggatgta agagatgata actctttctc 71940caaattccct aaaagaggtt tcatcaagaa atctaaacca gacttagatt tcgacatctc 72000attcagtttc tttaaaagag aatccagaaa gctcaatcca ccaaaggtgg ggaattgaga 72060tggagtaaat tttaaggatt tgtagtacgt ctcaacttgt gccttcagat ctttagtctt 72120ctccaatatc tgtatcgagc aaagacttat tttgctagtg tcatctttgt ttatagatct 72180aggaacaagc ttttgaatta catatagaac atcacccgct atagctccaa cctttaacaa 72240gacctcattc aaccagttac cattaataac gtgatttgac acatcagcat caaggaaaac 72300caacaagaat tctattgcca catcaatatt ttgagcagag atattattag gaaagttctc 72360gagttttttg tttctgagat acatcaacag attgcagaga tgatgtgaaa attcttttgg 72420taatttcttg tccttaaaag tttttgattg agtaaacttt gaagccatta gttcaccatg 72480aaaaatcttc ttcatttcca actccgcaaa cacaatcaat gataatagat aaggaggttt 72540actaaagatg tcattttctt cattaacttc acgttcagta acataatcta atacggccaa 72600acaatattgt cttacattgt tagtcatgaa ctgaattcga gtctccaaac attccagctt 72660ctcatagtcg acataaccat ttatctctgt ggcatatatg tatttcaaaa atctcatttt 72720cttttgaaca attttcagtt cctttataga tcgatgtatt gcaagttttt ttttggaatg 72780gcaggaccta cccttttgca agaacatcat cagtacatca tttagaattt tttccagaca 72840atccatatat ttcgacagat caaaatcatt caactcataa ttgtaactta aactggtatt 72900accttgaaag aattccaaca aatgtgattc tagcctttcc agattaaggt tagttttaca 72960ttcatctgga atcctatgca atacccggtg aagtatttcc ccagtcaatt tggcactctt 73020tgtgagttcc accaaggaat caggcaaaag aacatcatga tactttataa aggttctaaa 73080aactcttagc accatttcaa gttccttaat tcgcaatatc tcgaaccaat ccagattacc 73140tccactcttg atccttctca ggtggtctaa catttcctca atttcatttt cagccatttc 73200tgtagaaaaa ttcctgcaag ttcaccaact tttggaatta ggttcttgaa gccatcttaa 73260tgcttttgaa aagttataaa tatttacaat tttacatctt atttaccctt ttcgtattgt 73320catttaacta acaattatta tcgcataaaa tcgtcttttt tcttgttgtc atttatgaga 73380tagtaactat tagttgagtg gaccatttga gaaatcaact ctctaattat gtatctatgg 73440gaaaaagatg acttaccgtt agttaacaag tatcttcaat ttctgatcca atcataaaag 73500ggagcaatag agaaaaacaa acattataag gtaaaccaga gagtaataat tctcagattt 73560ggattccaac aaagatatat ttccaagttt acataatagg tgaaacaaga aaaaatttat 73620tgaaataaaa tggatgaagg tttgaaggaa gaaggaagag tcttcttctc tctctataat 73680atttcttata cctaacaaga gatgaattaa gtcggtagct gtaaagtgac tcaataatac 73740acccatatta tttgtctttt tctttagggg ttgtttggtt ggagcaagtt atttcgggat 73800taattatcgc gaaataagtt acttgatcat gtatatgtga tcataattta tatattccat 73860cactatgata tgggatacat aatcggagga ctaccaaata tggctccaac caaacacaag 73920ataaaataat tctgcatttt atcccaaaat tattatatct catacttcac accaaatgac 73980tctttaggaa aatcccccaa aattttattt ggtttggcag tttcatagtt aaattatgca 74040cttatatgat atttgcttag catataatta aggaatttta tggttgggta ttgggtcgga 74100catgaagtta tattttttct aataaatatg aaaatgcaca agttgtgaaa actttgaaat 74160tcaattctta tgcaatttta ccaaatgagt aaatcatttc gttaacaaga tatcataata 74220tctaaagcat cgcatcgatg aattatatat ctttatgttt cttttaactt tcaaatgtac 74280ataaacttat aaagatccac tagtcaacaa tagtagtaat tagtaattct ttcacatgat 74340acgacggaca atttattcac gtcgaacatg agtttatttt tgtaaaactg aaaataatga 74400tttgttcttt tttacaaaat ataaacttat gaataagttt ttatattttt ttaaaaaaac 74460ataattcaaa aaaggataaa tcatatgtca taaatcaaac aaggataaaa aaggagtggt 74520gataattctt ccatagatct tgtgaatgga gtctgttctc agaacagggc caatcataat 74580acaaaagatt gatttttttg gttcttaagg gtcaaatgcc cttatttatt catttcatgt 74640cgtactatat acattttttt gtttccaccc aatgtccggt acccacattg gagcccgata 74700caatggattc acgacgtaat aaagtcccac attgggggtg aaacgctccc taacatagac 74760gactctgtat tcggagggga ctcgaatgtc atactatatt tttactatat cttaagttca 74820tatgttgcaa agttcaaatg atcgatgaat gacaagaaca aatttattag cttcattctg 74880tatgaatttc aacttctaaa agaggcacct gaacacataa taaagagatc cttcaacatt 74940gcacctacag caacaagaag cagtttaacc ttaaaatttt tcggatgttc atcgaattag 75000aaaatcaaga agtactcaac aacaacaaca acaacaacat ggttgatgtg tattgagcta 75060tgaagatgta agtggagaga aattggtcaa aattataaag ctgcataaaa acacgaaatt 75120agtgaaagac gatctgatgt atatttgtct ccaatcatat catacataaa aaagagttga 75180tcaatcaaaa caaacatagc aaacactact agtgttacaa taacagaatg agctagcaac 75240aaaatagact tcagtattct ttgacaaggt ctatacggtc acatctttca atctcttcga 75300cttcttctta cgaggtctat acggtcacat ccttcaatct cttcgacttc ttccttaatt 75360ctcacagctg aagcctccat agatactttc caagacccaa tcaacttaat ctgtttcaat 75420gatggaatat cagcaaagct aagtgggatc tcctcaaggt catcacactt ttttataaca 75480agtgtttcaa gcaagggaaa tgattcctcc gaggcatccc actttgagat atttaactgc 75540accagtttca acaacttaag tttatggaac gtgatatctc caaggcacca ctcttctgat 75600tgaggaaaac acacatctag taattggaga tactccaggc ttggtagttc cgcaatgaag 75660gaaatagcac tttccatatg aattccttta agtaccaatt tgtttaaatt tgaaggcaac 75720tgtaacccgg atagaatgcg gggatgcaca aaggaaagtt gaagttcttg aagctgggta 75780agactctcca atttgggaca aaaaggctct acatcatcct caaatgtgat ttgaagttgt 75840tgaagattag gacacctcct tgataacaca tccagcctat caattggaaa ttcctcaatt 75900ttctttaata tcctcaaatt ttccaattta gaggatcctt caaagagtcc ctgcatagca 75960aaaccagcgc cagcaaaatg aacatgcctt aatttttcca tttcccaaaa agacactggt 76020aaccgtacac cataaaaaca actcgtcaca attaaagttt ctatatgggg cagatgtgat 76080tgtggatcaa aatagaaagt acctgcccaa actgcgaggt acttcagctg attaagtggg 76140tttaatctag ctaaccacaa agactccaca taataagaac tcaaatccaa gaccttaaga 76200agtcgcaatt cattaatctg acagacggga atcacatcaa tagattctcc accattagcc 76260gttatcagag acctcaagtg

ttggtggaaa ggcttctgtg ttttggaaac cagagatgca 76320aacttggaaa gctcttcact gaaactgaag ctcactcgac ttcccttcca atccaacggt 76380tgaaactgga tatattgacc cttcactgca agcataaact tttcttctct actcttcttc 76440aagcaaaagt gatgcacaac atcatgaacc tggcagcatt tgactttacc attataacct 76500ttctttgaaa caattaccac gttactgcta atgagatcca tcaagtaacc ttcagcttcc 76560tcttccatta atctcccaga ttcagtgttc tccacgaatc cttcagcaat ccataagctt 76620atcaatttag acactggaat tcttgcgtcc tccagaaaca tccccatata aagaagacaa 76680ggctttaaac aatcagctag gttatcaaaa ctcaactgca tagtcgctag actgtattct 76740tcgaactcac tgtcaagata gtcaaataaa gcatctttca cctcattcca ccaagattct 76800tccattttcc tttttttgat tattccagct accaagacaa ccactagggg cagtcctttg 76860catttttctg caactgcttg actcacatat tgtagttcag gcgggcaatc ttccttttga 76920aacacttttt tctgcagcaa ttggcaactc tcttttgttg tgaggaatgg aagagaataa 76980ggatcagtat ggttcttgac ttgcttaccc acttcttcaa gtcgagttgt tacgactatt 77040ctacttctga ttccatcatc tggaaaagaa agccttaagt catcccatac catacaatcc 77100cacatatcat ccaatacaat gagatatctc tttcccatta ggcttttcct caacctgtca 77160gcaagtttgc ctacctcatc ttccttgtcc ttggagcccg taacttgact gaaaatctct 77220tgtaatagct ctcttcggct atatgtttga gaaatgatgc accatgctcg aacattaaag 77280cgagaaacaa tgatgtcatt attgtacaac tttctagcaa ttgtcgtttt cccttgtccc 77340cccatgccta caattgggat gacgtctagc tcatttgtac ctctaatcag ataaccaatt 77400agtttttctg ctttattctc aaaacccact acctcctcat cagtcacaag attgctatgt 77460cgagctggca gatgtttaaa tggagcagcc acgtagcgag gattaagagg aatgtttgct 77520gaccacatct cagtcacctc tgcattaatt tgcttgatct cttttaaaat tgtaggaagt 77580gagcaaaaaa tatgcaaaaa agcattatac tgagcaagaa tagagtcaat ggcaacctca 77640gcctcatatg ccaaattgat ggtacgtctc tgaagatctt taggaatttt atgttcgtgg 77700tgcacgacat ctctgaaaat ggatgatagc tccttctcta aaatggatgt aagagatgat 77760aactctttct ccaaattccc taaaagaggt ttcatcagga aatctaaacc agacttagat 77820ttcgacatct cattcagttt ccttaaaaga gaatccagaa agctcaatcc accaaaggtg 77880gggaactgag atggagtaaa ttttaaggat ttgtagtacg tctcaacttg tgccttcaga 77940tctttagtct tctccaatat ctgtatcgag caaaaactta ttttgctagt gtcatctttg 78000tttatagatc taggaagaag cttttgaatt acatatagaa tatcacccgc tatagctcca 78060acctttaaca agacctcatt caaccagtta ccattaataa cgtgatttga cacatcagca 78120tcaaggaaaa ccaacaagaa ttctattgcc acatcaatat tttgagcaga gatattatta 78180ggaaagttct cgagtttttc gtttctgaga tacatcaaca gatattggag aagatctgaa 78240aattcttttg gtaatttctt gtccttgaaa gtttttgatt gagtaaactt tgacaccttt 78300agttcaccat gaaaaatctt cttcatttcc agctccacaa acacaatcaa tgataataga 78360tacggaggta tattaaagat atcattatta tcactaaatt caatatcagc aacataatct 78420aatacggcca aacaaaattg tcccacagtg ttagcaatga actgaattcg agtctccaaa 78480cattccagct tctcatagtt gacgttacca tttatctctg tggtatatat gtatctcaaa 78540aatatcattt tcttttgaac aattttcagt ttctttatag atagctgtat tgcaagtttt 78600tctatgggat aacaggacct acccttttcc aggaacatca ttagtacatc atttagaatt 78660ttttccagac aatccatata tttcgacaga tcaaaatcat tcaactcata attgtaactt 78720aaactggagt taccttcaaa gaattccaac aaatgtgatt ctagcctttc caaattaagg 78780ttagttttac attcatctga tatcccatca aatacccggt gaagcatttc cacagtccat 78840tcggcattca ttgtgagttt gacaaaacaa tcaggtaaaa gaacatgata ctttataaag 78900gttctaaaaa ctcttagcac aatatcaaga tccccaatac gacgaatctt gaagaaatcc 78960aggttacctt caattttgat ccttcttagg tgatctaaca tttcctcaat ttcattttca 79020ttcatttttt gtagaaaaat tcctgcaagt tcaccaactc atggaattag gttcatgtct 79080ttcaatatct agtgtttgaa gctatgttaa tgcttgtgaa aagttataca tcttatgtac 79140cattttcata ttgtcaaatg tcacttaact aattattatc tgagaaaatc gtctttctta 79200cattttcaat tttatgagat actaactaat agttgagtga accatttgag aaatcaactc 79260tctaattctg tatctatggg aaaaaagtaa ttcatgaaga agatgactta cagttagttg 79320acaagaatct tcaatttctc agatttggat tccaacaaag atatatttcc aagtttacgc 79380aataggtgat ataagaaaaa aattcattga aataaaatga atggaagttt gaaggtttga 79440aggaagagag ttcttctgtc tctctaataa ttcttatacc taacaagaga tgaattgtag 79500tagcaagttg ctttaggtgg tagctgtaaa gtgcctcaat aatacaacta tattacttag 79560tccttttttt aagggtcgtt tggtcgagaa taagttattt tgagagtaat tatctcacaa 79620taagttattt aacatgtata tgaaataact tattccatca ctaaagtata aatattggga 79680taaataatca aaggactatc tagtacctcc aatcaaatac ataataaaat aatcctatat 79740tttattccaa aatttttata cctcatacct cacgccaaac gactccttag gaaaatcccc 79800caaaattatg tccgatttgc taatttcata cttaaactat gaactatttt gatatttgtt 79860tagcatataa ttaaggggta ttgggtcgga catgaagtta ttttttctca taaatatgaa 79920aattctcaag ttgtgaaaac tatcaaaagt tatctaattc ttaagcaatc ttaccgaatg 79980agtaaatcat catttgtaaa taagatatca aaatattcta aagcatcgca ttgatgaatt 80040acatattttc atgtttcttt tataaataaa tattcacgat tacaaacttt caaatgcaca 80100taaatttata aagatccact agtcaataat aatagtaaca aataattact ctttaagtta 80160atattttcac ttggcacgga caatccattc aaatagaata tgagtatttc tataaattta 80220aaatgatgac tccttttttt atatatataa attattaact tatgagtcag atattattta 80280atatttagaa aaaggcaagt tgtatcagca agttgattta ggtggtaggt gtaagattaa 80340tgctgtctcc ttctttaaaa tagattgaaa ttcatgtatt agactagtat tcacatgctt 80400tattgaccaa ataatgtcag aacaattaga ctaagaatag tcatcgagat ctcaaacagg 80460gattaaaaaa agtagtggtg ataattcatc catacatctt tcgggataac acatatttac 80520caattaaatt aggcttaagt cattcacgat tccttaaaag ttatttgtat atttcagtta 80580gacatcttaa ctaggactat tacctattga acacttaaat tgtttaaaaa attacctatt 80640aaatacaaaa tgctgatatg acaaaataag tgtttttcac ttcctatggg tgcgtgaaag 80700tttttgaaat actgttttat ttttattttt tccaaaaaaa cttacacttt ttcttcttta 80760agtgacactt ggcatttaaa taaactaaaa taatttttta atatttttta aaaaaaattc 80820ttaattcaaa aaagaaagag cacccctacc cttcaagtca tcttcttctc taaatttaaa 80880ttttcaaaga caactaaaga atcttcattt tttttgtttt tgtttttgtt tttccatttc 80940ttttcaatat tcgtaaaaaa aattgaatca agtaactgaa tttgcaagaa agataatgaa 81000agtagatttt tgaacattgg gataaaaaaa gtaagtcgac gctccatctc cattttcggt 81060caccttcaaa taaatattac cattccgaga aaattgtaaa ttcactaaac ttatcaaaaa 81120caatatcaaa taattcgcta aattaattta ggtcctcttg atttctcctt acaatcaatt 81180tcaagatgat taaccattag catacttcct ttattcatca ctcttgtacc aactctttga 81240atatctttct ctatcatgta ttaggaatga aaaataattt tcgataaatt aatttgggtc 81300ctttcgattt ttctttgcaa tcaattttcg gatgagtaac tattgacaaa cttcttcatc 81360actattgtat caatctcttt gaatattttt ctctcacaca tattaggaat acaaaatatt 81420ttttttaaaa aattgaatct gcaagtaatt tgcagtgaag gtttttgtgg tttgagaaaa 81480aaaattgatg agaagaagat gaagaacaaa ggggggttgg gtagggtggg gttaagtaaa 81540atgggttgtc atttcattta tttttttatt aagaaataga tataaaattt atttgaaata 81600attttcattc tttttaacat aaaaaaaaat aaaaatctat agttgttggg gtcaagtgac 81660acatgtcatc ttttaattag ttattttagc cacgtcatag caagtgtatc acacacactt 81720taactttttt actgattatc aaaaaaggtc taataggaac aggttttaga tagataaaag 81780tgttcaatag gtactagtcc tagttgaagt gtttaagtga attatgcgga caactttaag 81840gggccgcaga tgacttaagc cattagatta tgatcgaaat tccatagaca caccttaact 81900aaatcagggg cgtaggcaca tgtatgtcag ggtgtccaat tggacatcct tagttggaaa 81960aaatttcgca tatacaagta aaatgataca cgaaatgatt aaataatata ttttggacac 82020ccttgacata ataggttgtt atagcctagt gatttaagat gtcttgaatg tgtgtttata 82080catctttaat gagtcagtgt ttgtgtgttc aaatctcact tgcaacaatt tttttccctt 82140tttaaaaaga acttttgttg tttaattttt agaccctctt cgtgaaattt ctagctccga 82200cattgaacta aaactaaggt cctattatcc cctgaactca tttttttgat aattttgtac 82260acattttagc ttggcatatg cactccgtga ctccacacaa ttgaggtgcg tgggagatat 82320tttaatgcca caagccaaaa tgatgtacaa aattaaaaaa aaacatgggt tcaggagtaa 82380taggacatta gtctagttaa gttgtgtctc tgagattttg gtcatagtct agggaggtat 82440ttgtaccttt tttccctaca tcttccttgt aaatggaatg tgttaccaat gagggtcata 82500gtggagtgat tagtattcct tcgtccttaa ccagatgcct tggattcgaa tcccccttga 82560tacataatcg tctttgttaa ggagcgtttt cccgtatact aagtatgtta ttgttattgt 82620atcgatagag acaaccagat ggttcgaaac cactaaaaat caccatcttc agataaaaga 82680aatattagat gattataaga aaatcctgat atgggcatat gaacacataa aataaggcca 82740taaagagatt tactctagaa accaatttag acaagcaaaa catatatatc tggcttacca 82800taaacatgac caaaaagttt cttaattaat ttccaggacc tgagcctaaa tatattctct 82860ttgtatcaaa caagaaatcc tactgaattt attttcttct cagaactaaa aatgccttct 82920ccacttggcc tccaaaagag ccttccatat cacaacttga tcccaaagtt gatgagaagc 82980aagctatata ttacgaacat tgaagttcaa cgtgttaatt tccctgaaag tacaatcgtc 83040aaattaatct ttttattagc taagaaatga attccagcta actgtctgta gttgtgtaac 83100aagtatcaca atttgatcaa tcttgacgct attcagattg cttacactct tcatccttct 83160tagctgatct aacatatcct caatgtcgtt tcgagccatc tttcgtagaa aattcctgcg 83220agttcaacaa acacttggaa tattaatttc attaattatg agcaacattt ctattcgtgt 83280ctttcaatat ctgccacagg aacatgatct ttattatagg ataagaagtt taaagtcgta 83340catttttagg aggtcaatca ttatgtgatt agtaaaaaaa tgagagaact gtagagaata 83400gcagaagaaa tgtataattg ggagaccttc tactagccaa taattcgagt tttcaataga 83460ctggaattat gaagtctcta ttattttata tatatatata tatatatata tatatatata 83520tatatatata tatatatact ctatgataca gaaaaaggag gagagagcag agtacatatg 83580tacatataag ggaaaaggag aaataataat cctcatcaag actttatagc cacaaacttt 83640ttgacccccc aaatcttccc tgtgggtctg gcctatgttt aaaaattaga atataggagg 83700accattttat aacttaataa tcatggaatt aagtaagcgt tacgaaaatt tagcaactca 83760ggtcgaaaat tcaaatcatg ttttcttgaa aagattgaaa tttgaaatta atctcaaatc 83820taaactttga tttggaatca tatcttcata ttacatattc aaatgtgagt tcaagtttaa 83880atatgtccag cttgtaataa ttttatcaac ctaacataga cattgacaat cgtgagattt 83940ctagaagatt agaagaaaaa acaaccttga aagagaataa gtacatttca ataagaaaag 84000aagttttagt agctcgattt tatcgactaa ctgaattctc attttattga tgaggatcac 84060atttcattcc atcattccaa aagaaaaaac ctcaaatggt taattattga attttcaatt 84120tgttagttat ctaaactctt tcacgaggat ccaatttcat tctctgaagt tcttcagatt 84180tttggtaaaa ttcaattcat ttcagaaaaa aaataatctt aaattcttaa aaaattgaca 84240catatggtaa aaattcgatc catttcaaag aaatatacta tatttatgta tacaacaccc 84300actatataca gctatatagt gttggaaaac aaaaacgtag agaattattc attcatcaac 84360aaagtatatc cacacacgcc aacatatata tacaagtcct atatatcaca aaagtgaaaa 84420taaaagttga acaaaacact agacgatatc cgacttcgct agccaaaacg ctagcgaaaa 84480cgaaatgaac atatgagcaa tcgaacatga aaacgagatg aacatatgag caatcgaacg 84540cgacttcgct gtcggagctt aaacaaaaac tctagggaca gattcgatag gtttacaatg 84600aacttctcta aggagagtgc gaatgccttt gcctccagca ttagcaagtg ggtgaattcc 84660atgggcagga ccttccatgt 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ttttagcttt tggtasaagt gtagtgacca atcagaggaa 88920acttttctta ttcaccaatt aaaactatat gataaattaa taacaagctt ttgaaatgta 88980attcttattt ttagagttta ggataaaaaa ggagtatatg atttaaaata tctcattttt 89040tcaaatttta tacctgaact atctgtatgc tasttttcta tttgaattat cacktaagct 89100tgaatcwctt ttctattgaa atatcatcat tgttcgggag caactgatag ttgtggtttg 89160ttttgataaa tagttgatga tagtttaggt gaatactcgc acaccgaata gttaagatat 89220aaaactcgaa aaaatcaaaa tactatagtt gtgttttgac cctttactct tcttcttttt 89280ttaatgtgaa actcaatgga tctttactta cttttataaa acaaaactta tacactacgc 89340atggatgaat ttagaagttt aaatacagat tcaattgaac ccaatagttt ttgtataaac 89400gatacatttg tgtgaagaat ttcattaaat atatattcat attagattta aagctcaatt 89460attattattt gaagtcgtta ttctataatt ttaaacccat aaaattaaaa tcatgactcg 89520ccttaatcat cacatattaa tcaaaaccac tcgcgttaat taattatgtg gatataatat 89580ccattgtttt aacctaatat ataatcttaa atatgaactt tttatatata aaagattgat 89640ttcttttgta attgtgggga aaagggagga agttataagc aacttgtaaa ttaatataat 89700tgaaccctag tttgcctatc atccatctat tcattcgagg tgggaataaa aaataaaaaa 89760attaaaacct tcttttatta aaaaaatata accttacaat tatagtagtc atattcttaa 89820agggaaaagg gtgcaacttg tgcaattttt agtacactta attaggtcat atacatgcat 89880ctagttgttg aatcgatgtg agagttgaaa taaattatta ttaagagaga atagtgggaa 89940aagggttgaa atttgtgtaa tttagaaccc ttatttgtac catgtagcta ttcaatcgac 90000gtaagagtca gaaaattgtg ataacagtca gacgacactc caaaaaataa aaatatataa 90060gggtacaact tggatgattt aattttttaa attttaaatc gtctagtcat tgaactcgag 90120tttgtataaa agaatgattt gtacttacgt atagtgatat aggtcatcaa ctgtcaatta 90180taatatagaa tattctattt ttttttattt gaatgaaaca agaagattta aatgaaggat 90240attcttttta ccaataatat aagctagaat tagtaagttt ctatgataag caattaatgt 90300ttttttttct tcttcttttc taatctctgt gggcatattt atgagagtaa gggtatgttt 90360ggcctgttga agtttggtaa ttgagtattt aaggttcaaa aattcaaata aagttagcat 90420atgactttct ggagtatact tttttctaaa ttataaacta tagtgtttaa atttatgtat 90480aaaaaaaata ttagtgttca aataaggtaa gcataggact ttttgagata gactaataaa 90540tattttttca gaaaaaataa taattttcac actaggaaat gtcaatatct aaaataataa 90600taaaaatact ggacatggtc aatacaaata taatacacag gacatactat gttctaggag 90660aagtgcatat gaacatgaaa aagaaagaac tattcaatat gaaaaaataa aagataatta 90720atcttctaat tatttatcaa ggaagtaatc aaagtgtcat atataattat tagtagaatc 90780ctaaaattta caaatgatga tttttttaat tatgaaaaat agtttagtga ctttatatta 90840cttatattaa atagaatatt tcttaaatca caaaagttat actttctcta attcacttta 90900tttgtcgtat tttattttgt acacaatatt aattaaagga ttatttgact aaattagctt 90960tgtttattac tccttttaat ttaatatata cttccctctt tgtaaagtag caatatgcat 91020gcacacttat taacctactt gtatatatgc catcaaaaca cttaattaat gggaccaatt 91080catttctcga ccaataatat cataacttcg acattataat actctcttcg tattaattta 91140tgagacatct ttcaaatttc aaatgttaaa taattttttt aatcactagc tttttatatg 91200tcttataaat atttaaatca ttaataaaaa gataattact ataatactat ttctacaaat 91260ccttgtggaa tacaacaaac ttctcgtgac ttgagtatat atacgtgcaa ttgctagtat 91320agtactatat agtgttgctt

cacatgcatt gtacaggatg ctttgacaca ccacattgag 91380cagcaagttg aacaatatgg attgaggccg ggtcaggcct gcacctgttc ggatcgaccc 91440ggtgaggacc cgccaaatga aacaaccaat gtagaagagg aaggggccgg ttaattaggc 91500aaagtatgtg tctgtggaga gtagattgta tgaatagacc ttcgttacaa aatgaatatt 91560atgatcaaag caggatgaca agaaaatatg acaactgaat aggaagatac aagcaaatgt 91620agtaagagtt cgatagtata cacataaaag aataagaaac tacatgatta ctaaatacta 91680ctactaaaag cgagaagagg gaaggagaag actggcccca cgtctcccat acatatagta 91740cgagaatact tggatatcta ctaactttct actttaatca tcgatctcca gactttccta 91800tcttgatact agctatgatc acctcgatcc ctttagttct ttttcaacct acttttacat 91860cccctagctc ctactatagt caacctctca taccatctac ccgatgtatc tgcacactcc 91920atatttacat gtttgaacca tctcactctc atttccctta ttttgattgt cgcaaaagcc 91980actcacatat tttccctgta taactttatt cctaatcata ttcctcatag tatgcccaca 92040cgaccactct tctaaacgtg gatattcttg actagctagc cacccctctt cccaatacaa 92100caatgtcgat ctaaccacta ttctgtaact tacctttaaa actctactac atattcttat 92160cacacaaaac ataccacatc gtgatccatc tcctcgttct cttgaattat gaattcaaaa 92220tacttggata tctactaatt ttctacctaa aagtgagaag agggaaggag aagactggcc 92280ccacctctct cccatacatt tagtacgaca atataacttg gatatctact aactttctac 92340cttaatcttc gatctccaaa ctttcctatg ttggtacaag ctataatcac ctccctttag 92400ttcctttaat ttcaacctac ttttacatcc actaactcct gctatagtca acttctcatc 92460ccacctaccc gacatatctg cacactccat cttcacatgt ttgaaccatc tcaatctcac 92520ttctctcatt ttgattgtca caaaagccat tcacatattt cccccatata actttattcc 92580taatcatatc cctcataata tgcccacacc ccatctttcc ccgtataact ttattcctaa 92640tcatattcct catagtatgc ccacacgacc actcttctaa acgtgggtat tcttgactag 92700ctagccaccc ctcttcccaa tacaacaatg tcgatctaac cactattctg taacttacct 92760ttaaaactct actacatatt cttatcacac aaaacatacc acattgtgat ccatctcctc 92820gttctcttga attatgaatt caaaatactt aaaacttcct ctctttgaaa taactcatgc 92880atcatttttc atgaattacg ttattaaact tacactctag ataaaaataa taattcacga 92940catgatctaa taacgacaac gtgccacgta aattgaaaca gataaattat aaatataata 93000atttagtagg ataggatgta gtacacattg tatattaatt ttctattaaa aaaaaagaag 93060gtaaagacaa aatcacaaaa acaacaaaac cccattggct gatccactct ctttctcaca 93120agacttttca tcaaacaagt gtagcacttt ttcatttttt ttattcccaa cttgctcttc 93180cctcttaccc cctcacccca cccacccacc tctctctcta ttaagttctc cacctccaaa 93240tcttaacaat ccaattcaaa acaattctca aatgtatcca tcatcaacat cttcttcatc 93300acaaggttca atgagccaca ccagcaccac cgctggcggc ggtggcggtc tcactcgcta 93360cggttcagct ccgggatcat ttttaactac agcagttgaa tccgtcgtta acggtaatca 93420cgagttcgct tctcatggat ctcatcactc aaaccttggt ccttcacggt ttttcccgtc 93480caatttagca tctaattcgt taaattctga atctacaagc aaagcgaagg aacaatcgaa 93540tttacagagg tcaattggtt tcaacgactt aacaatcggc ggcggcggcg gaggtttgcc 93600gacgacttca accacgccgt tggttcgaca tagtagctca ccggcgaggt ttcttaatca 93660acttgctact gctgcaggtg atactggtaa gttttcaact ttcctttttt cctaattgaa 93720atgtgaaatg acgaatttga ccttcctgta tatgattcct tctgtttgct tttttttttt 93780cccctgctaa agtcttcagc ttttggtaac tttttaacca atcagagcag actttgctaa 93840ttgactaatc acaaccgact ttgcataaat taacaaactt tgaaatgtgt tttacatttt 93900taagtattcc tttggttata tcttctagaa acaaaattta agaaagaaat aattttttct 93960ttttccatgc ttaaacatgt taggacaatt ccatagttat tcgagtatat cacattacta 94020agggtgaaat gagaagttcg aaattcaaaa aatcaatttt gttgaaatag actaaacata 94080taagagtgtc gtacaagacg gagtcttagt tttatactag tcaaattcag ttaagatgct 94140agttgtgtag taaatgatta aaatttgacg atggataaat tagaaccacg agttcatgag 94200ttcaagttgt ggaaacaatc tattacagaa atgcaaggta aaattatgcg caataaactc 94260ttgtgaccct gacatagcag aagcttaatt agtgcatcga gctaccttta aaaaaaattg 94320taattagcta tagatgaaaa gagaaagtag gtttcttgca gatagaacca attaatggag 94380gattaactta tattgattat cattggacta tattaacttc ttaatgaatt gtcatgagat 94440tagagtacaa aagtaagatt tacttatgtc acaattagac gttataaact gactagttga 94500gcacttaaac ataattaatt actcaaaaaa ggacacataa aagagtaagt ggggaaagat 94560gcttagagtt attgaatttg tcttatctaa ttatctaatt acatacaaca agttgctaaa 94620aagaattacc aaaagattaa aagagagcta tgcaaatgct aaagatatac ttggtactca 94680aagaatcttt aatacctcct tacaacttgc aaattctttt ttttttcttt ccttccatat 94740gattagtact tttaacattt aaacttagaa gtacttcatt tgtctcactt catgtagtta 94800ctaaattggt gagttaaaaa ggaattcttt aaatattttg aattatgaat tattgaaatt 94860atagtacata tcaagtaatt tttaaatatg taaaaaaatt atttttaatt tttttaaaat 94920tatgtgtcca tcaaatattg aatcatctca cataaaaaag tatatcatat cgaccaaaag 94980tttagctagt tctacttttc ctatggtgag tgtcattttt gtgtctattc tttgtatctg 95040tctacgataa taacaataac atacttaaca taatcttata actgaagtct cgaaagaaca 95100gagtgtacat atattttatg tctattttgt agaaattaaa aggctgtttc ccatagactc 95160tcgacttaaa taaaacattt taaaaacagt ttgatttttt tttatctgtc taccttctac 95220aatttccaac aaaactttcc aatcttgaca aagatgtcaa aaccagattt cagacttcag 95280tagttcgtgt ttgtaaattg aatccatagt ataattcaag gattatactc catctcatcc 95340caattatcat tcgtttggca agttgaagtt atttaaaagt attattcatt ttaggaaaaa 95400acaaagaagt gattttgcta atccatccgt tccaatttat gtgtcataat ttgattttgt 95460acaaactttt gaaaaaaaag taaatacttc taaaagtttt ggttttaaac atcccaaaac 95520atatatgtga cttttataag acgtgtatac cataaaagct tcagattaaa aacatgtcat 95580tatttctgaa acatataaaa aagaacctga tagttttggc caaaattata tatttatctt 95640gaaaaattca ttcaacataa ataaattctt gtttacaacc aacttaatga tttagaattc 95700gaaagttata tacttcaaat atagttaatt agtataaaaa gtagagggga acaatcttgg 95760gttagcttag agtaaatgga attagtttcc taaattagaa ggtatataac gtaagcagcc 95820gcaaattatt attgtttttt cttaaacaaa ataacttatc catcaatagg gcaaattagg 95880agattagtgg agctaattgt cagtatttct taatcctgtc tcaactctta gtgacatgta 95940aaggtgggtt ccacatttgg ggaaagtgaa tgcatgtgtg tctgctaagt ttccaatcgc 96000ccacaacatt tggctaatga aaccactggt attaattaat tactcattaa tcatggtgga 96060tccttcttta agatagaatc tcattagcac taattaatat atattttttt aaagtttttg 96120gaggtaggat ttgggggttg gtttggggtg ggggtagaag ggagttagat gcaacttgtg 96180caatttgaca ctcttgagct tataaacaat aatatattca gtataattgt gaggtttgaa 96240gacgataaag tgtgtgtaca actttaaaaa gttattttca atagatcctg acacgataca 96300tctaagttta tatatcaaac aaatttgtta ccttttagtt ttttataact attattattt 96360aaaccaactt gcatgtatct aattaattac atatctagtt aattatataa gagtatttgt 96420tacctcatgt caacacatac aacaacatac ctaacaatta caaagtaaat ttttactagt 96480agagtttaga tagggtgaaa tgtatgtagg tcttattgaa atagaaatgt tataagagtt 96540aaattttaat tctttttagt ctaaggagta aggggtttga tcttagtggt aaaagtataa 96600cgtgcgatat gttaagtgta gatcatgaaa ttttgcctca ggtaaaattt tttatattca 96660agtggagaag atcagaaaga tgaattcatt atcctttgaa ttgtgaatca tgcatcactg 96720atccttgttg ttttcgattt aaaaaaaata tttgaccttt gtattaatat aattaatggt 96780gcctagatcc ttagaagcag ggaagggaga aggaatgata ctacttgtgt gaattaattt 96840gatatgaggt gggagacaga aaggctatgc ttttattgtc aactatcaaa aactctacaa 96900ctcattttgg tcgatttaat taagttgctt gaacttttca aaattgttgt tgtatccgtg 96960tcagattctt taaaaatgta ctattttaaa gatctgacac atacccttct acatttttga 97020agaatccgag caatatagaa atagattctg ctttcatcta aaaatgtggt ttaatctatg 97080attttttgcc aacaatatat gttgatttgg aataggacat acaatgtgtg tatgttttga 97140cctcgaaata aggcaattta ttctctatta atttttttta ataattataa tctactctag 97200gttaggttta ggtctgctat tagaccttta atcgttgtat tagtttctta agttaatgtg 97260tgtatgaact aagtcatgtt cattatgtca cacaaacttg aatgagtcaa taaagtggtg 97320ttggggttcc tttattcatt ttttccactt gtagaaattg atacattaga ggttggtgtg 97380cttcttgagc acgagtctat tccattagtt atgttgtagt tttctcttct tcaacccaaa 97440taaaatagta ataaataaat aaattaaaca agtacaactt gggatatatt actcgttttg 97500ttccatttta tatagcttca tttcttttaa atttattcct ataataataa taataataac 97560aaaaacatat aagtgtaatt ccacaaataa ggtctaggga ggataagatg taccagactt 97620tacccctacc tttgtgggat agtgaaactt taggctaaaa aaaattattt gaagtaagat 97680cgtaagaaaa atatttttaa cgaaatatca agatacaaaa tattttataa ctgtatagta 97740atgtaataga caaaattgaa gaacttttag gggccaaaaa atgttttgtg gtctttgtat 97800tttatacgag tattgtacaa gtagagatat aagtggccat tttcgaatta ttaagtagct 97860ttatagaaca agttgtcact tgggccccaa tgagcaatat agatattatt attttcttgt 97920ttccttaatc ataatcttag caagaatctc cttattggag gaagtgagca tttttttttg 97980gtggggtcca aactttcaaa tagcaagttg acattatata aaattctact aaagtaccaa 98040ctagccaata aaataatgaa taaagaaaag ggaaggggtt ttgtcccttt atttaattga 98100tggtgattgt gcctacgatt ctttgaatcc atttttactt attttgaggt caagatgcat 98160tcaccaaaca gctcctaaaa ttccatgact ggtgagttat cacgtgcatg ggtatttact 98220tgacccgtta ttcccacttg ctctatccat tattatcatc atatatatat aatttatatc 98280ttagccgcca cctaaaacca gcccaatgtt tttgagtgct ttactagttt tacaataatt 98340gatgtttttt taccagactc ctttttctcc ccttctaaga tattttctga aacttttatg 98400gtcaactgtt tagcagatga tcctattttt atttgttctt gtaatttatg aaccttctag 98460atagtaaatt gaaaatctta taagaaatga cttgcttatg atcttaaatt ttgtagacat 98520taaacaaact taaatgtttt gttagtttca caggttatat tttcacaact tctgaaaata 98580gtgataaaat ttaaatgatg acttatataa aaaaattatt taaatctagg acttttacat 98640ttactgttca cattctgttc tgtcgagtgg tcaaatgaag gggtttaagc accataaggt 98700cctaggttca gttcctaaca aagacaaaac actaggtaat tttttccccc atatatccca 98760tgaaatggag ctttagtaga tcagttcgtt ttgagcagag ggtctaccgg aaacaacctc 98820tctacctaac aaggtagggt aaggtctgtg tatatcttgt tgtcgttggt tttatctttt 98880gtttgagtag taatattcta agttgctcgg actctccaaa aatgatgcgc acccgtgtgg 98940gatccttcaa aaatacacta tttttggagg atccgacacg cacctgtcaa cattttgaaa 99000gagtccgagc aacataggta atattcatac ctagtcaaac aaagacttca atttagttat 99060cgaatgaaag tcgaacaatt tgatgtggaa aagagaatct ctttatgtta tgtctgtgat 99120atcctttaat tttgtcaaag aagatcttaa tatatagagc caaatgaaac caaatacttt 99180tccatggaga ctacaagggt cacactagaa ctatcagctt ttgtggtgac atttttgagt 99240cacacccacg aaaagattca tatctaatat attgtaaaga ttttaatttg gtaaaaaaga 99300cctcgttacc ataactcgta atataattct agtttgactt gacgttagga ttttcagttt 99360caatggggag aggaagctat aactcaaaag gcggtggaga tagtggccgg ggaataacaa 99420ggctgaactc tcagctcagc ttcactaggc aagaagctct ctctcaaata gcagaggaaa 99480atgaggatat tgaagggacc agtatagcca atggccacag aaagtcaaca cattcttatg 99540ccagtgcaag tagtttcgca atgggttctt gggaagataa caactctata atgttctctg 99600tcacacctag caaacgagcc aagcagatta gcaatgacat ggtcaatggg ctcgatgatg 99660gggaaactca ggtaatataa ctgaaatttt gtgaaagtat gtttctctgg agaaattgta 99720tttttagttg caagtgttca agggtcatgt tttctttatc ctgaattctg gagttgtgca 99780tgtgatatgc ctaactttaa caacatttat gtgaaaaaat gagatgtcat tttaatgatg 99840ttagccttct agatgaaaaa atgtcttgtg ctttgatttg gtgacccttc aaaaagctga 99900cgatttgttt tttctcttgc attcttttat tcctaattcc ctacctttct tctttcttcc 99960agctaatatg aacttgacag tcactatcag aaaacacata aaccttcact gtttgacgtc 100020tgtgttttca acatgtcaac ggcgatagat atacttcctc tgcatttttc cccttaacaa 100080ccttactact acattctgta tgtgtttgtt ttctttctgc agaagaatgt aaattaaaac 100140agacaaaata atccatgttg gcatactcaa tcatgtttct cgtgcgcaac tctcgcaaat 100200attgatgaaa actgcatttt tgtttcttgt gagaggatga actgatgttt tacatttttg 100260aatcattttc agtttcagtt tggcttgtct cagacagcac tagaaatggc atctatggat 100320agattgctgc acatccccga ggattctgtt ccttgcaaaa ttcgtgccaa gcgtggttgt 100380gctactcatc ctcgcagcat tgcagaaagg gtaatcttca tatttcttaa gtcattcctt 100440tactcttggc ccttataatg aacaaagaaa taaaaatcca attgaacttt atcaatttat 100500ttttcccttg ctccgtttca ttttatatgg ctttgattga gaatgacgtt caagatacta 100560tctgaagctg atgcaatttg tccagccact gattttaaat ggcaattttt ccattatgta 100620acactagatg tagttatcaa catgtagtag ttgcggcatt tgaaatactt ttcctcaaaa 100680tttggatcat catagctatg ttttgttttt tctaatacgt gataatgaac attcttttct 100740tttcttttct tttcttatct tatgcaggaa agaagaacca gaattagtgg gaaactaaag 100800aagttacaag atcttgttcc aaacatggac aaggtatagt ttgctcattt cacattaact 100860tttgtgtgag agaagagtat cacgagaaat gtctttatgg tgttggaggg ggatgagtgt 100920cattaagttt taagccgaac ttgcagtata gctctgcaat tataaattat agctatctat 100980gaagtgcttc atatactata actctgtaat catgtttgct tggaacaaag aaaattagct 101040attggaagtg gcgtgctatt caattggata attgttactt gacgtgctaa aatgctgtta 101100aaccatcaat ttattgaata tttatagatt tgtgtaaaca aaaaattagt ctttcagtaa 101160tcttgattgt atgctttctt ttcttatgtt ccacttcaca atattttctc agttggcact 101220tattgcaaca tgcttgagct gcaaatttta tcgcagcaaa cgagctacac tccaatatta 101280acaatatttc cttgtttgtt tttgcagcaa acgagctacg ctgacatgct ggatctagca 101340gtgcagcaca ttcgaaccct tcaagatcag gttcaggtgg gtgattaaat tgatcaacct 101400tactgctttg cagctttacg taatctatca tgctgcatgc acctaggtac aaaattaaaa 101460tacttatact ctgttgctca gactcttcaa tgatactgtt ggatgtgtta gattttatcc 101520tccaaaagct atgcattttt ggaggatcag acactagtac aacaattttg gcccgagcaa 101580cacatcttat attctcacat gatgtttaca ccaaaccgaa taatataacc ttaatgtttt 101640tgggatagat aatgtctaat atattttgca aaaaaaatga taaattttct agccccacct 101700ttatagctgt gattgaagta gtagaaacat atttttcaca ttttcttttt cttatgcaga 101760atctgaatac agaacttgaa aactgcaaat gtggatgtaa gaaatcaagt caataacaaa 101820atggaaggaa gcactttagc tgaagatcga cttaaaagga tttttgtcac catgattttg 101880cctcttagaa actcaaattt gtacataagg aagtaaattt catactagtt ttttaaagct 101940aaaaggcgaa ctcttctttt tttttttttg ttttttttcc ttttccagtt agaagactac 102000gtatatgatt gttttggatt atttgtagtt tttttttttc catctaacat ttacattttt 102060aatttagttt tgagtttatt ttggttgagg atcataaagc taattgatga tgttgtacaa 102120caactttctc tatttcattt ttatgtaaaa tgagtgggta agacattttc ttgctttctg 102180ttatgttctt cttggaaaac gaccatttac aattttaaaa taagaaaagt aaatctactc 102240tacaaggatt tcattgtttt atttatcgaa ctgatcattt tctgtgaaaa aaactcattt 102300aattaatata acgtttgtat tgagccacaa aatcgatatt tcccttctta cccatgtata 102360tcatgaatat atgtgattca acaatgcggc gctttaggat atttcgatat tttgtttatg 102420aacgatgatt tgctcattcg gtaagattgc ataagaattg ggggattttt tttatatcaa 102480tgtgatttcg tatcgtatat ccaaacaaaa tttcaacttc atatcaatat gatttcatat 102540cgcatatcca aatggggccc caaatttgta aaaaaaaatt attgtacttg ttctattaat 102600caaatctttc attgtgaaag atgagcaaaa tgaattgagc ttgaatagtt aagttgaata 102660aaagttcatt gttgaactat tgaaaaaata ggtgaataag gtcatttgaa aagtcagtaa 102720ttttttgtta tttcactcaa aaagtcattc aagttcaaaa ataattctct gtcggaatct 102780gaatatgaga attattactc tcatgtttac ctcataataa tctgctttca attttaatta 102840tgattgaatc agaaaattga agatactaaa caggcataac agaagacaaa agttccaagt 102900tggtttcact tgttaactaa ctgtaagtta tcttcttcat gaattacttt tacctattga 102960tacaaaatta gggagttgat ttcttaaatg gtccactcaa ctaatagtta ttatattagg 103020aagccagttt ctttgttgac gaaaattgga attaaaaaaa gaaagtctga gataataatt 103080actccctccg tcccatttta tgtggcaaca tttgaccggg cacggagttt aagaaataaa 103140tgaaaacttt gaaatgttta ccaaattgac ccttcaaaaa atagtcatat taaaaaatag 103200acttactttt ctctctcctc ataagtgtat aattaagtgg gatcaataag ggtaaaagag 103260gaattgtacc tttaagtact taccatataa gaaaatgtga cattcttttt gggactcata 103320tgtgaaagag ttctcaaaag cttgaaaatc taaaaatcga ttttgtgaac ctatgaccct 103380aaaacccaaa caatgaactt gatccttcaa cacgcaatta caacttcaag caaacttcag 103440cacactttga aactggagtt ccaaagtccg aacaacaact caaaagagaa caatggaaga 103500attatatttg attttctttt tgtgataggg ataaacaagg aaaaacaaca ttattgagag 103560gaaggtattt ttgcaatgtt ggactgccat gtcagttcta atggcaaaga taacgtttta 103620aggatgtttg tgacataatt aaaaggtata tataagctat ttcaaatagt ttaagagtaa 103680ttttgaccat tttccgtata atatattata tatggaataa gacataattc tcaaagacaa 103740tgatagagtt gatggacgtt tttactaaag caatcaatag caacgaagca ctatcattaa 103800ttaataaagc actaattgtt gtttgcctac cattaattaa taaattaata atgcaataat 103860tcagaactca tacaatacac atttcactat gtttgtgcca ttgcaaactc taattattgt 103920ttgatctcat ttcccatctc tatgtactag atatagacaa caccttgtaa cataggattc 103980ggctatacaa tcaatcagtt aacaatcgat tgatctacta ttgagctatt gaggaataac 104040aagagattcg ataagttaag attaaggtga gttccattga ttcataattt attttcatac 104100taaaggaaat ttttatcctc taaatctata tgtcccccct ttccaattta agtgcttgag 104160tttgaccagt ggaagaggtt tggcttgttt ctctttggga taagggtcta aaaaataccc 104220caactttggt cggatttgct gttgcgatac taaactttca tgagaaccta ttacctccct 104280agactattta ataccgtatt ttaaacatat atatttgtcc acgtgacata aaaaataatg 104340caaaattata aatagtaatg tgtccatgtg ggcacatata tacctttaaa atacactatc 104400aggggataaa aaatacggta ttaaatagtc tagggaggta ataggtcctt atgaaagttt 104460agtatcgcaa caacaaatcc gaccaaagtt gaagtatttt tcagaccctt atccctttct 104520cttttttctg tatgtttcat atggtcttcc actgtaatgc cctatataag tatcatcact 104580tgcgtttgta gcttggtggt tgttttcccc tttaattttc tataatcctt tcatctaaca 104640ccttctatgc aaatatctca ccaagtccct cattgttctt tctattaatt atatttgtaa 104700caagttaggt acatatgact tgtatgcact agcttgagga gagtgttaga ataggaatag 104760gagcacgaat aatatataca attctactta gaataagaat aaaaatagta tacaatgtag 104820tgtcctagtt ggaaaaagag tcgaacataa taggtctctt gaggcattac taatatcatg 104880ccttttttca ctgtcatgaa aattcccagt cgcacccttt tcactacatg gagaattatt 104940ttgactagtc gaggaaagag ttcgactact agttgaattt ttgtgtgcaa ccagtgtcca 105000gtttttagtt gcattcttgt tttcaacttg caaaacatgc tgcccagaat tttcagcatc 105060ttcaacatca gatgtatcca ttagtttttg tcttgaatcc attaattttt gccctgaacc 105120aactcgcggt gcatcaaccc taacttttgg tgtccccctg cgtttttagt tgcattagac 105180atcaaagtca gcccaacttc ttttgattgc acctcattag tagcagcccg ttggttattg 105240caatcacctt tatttttggt agttgtttga acaccaatat tacttgtatc aacatccatt 105300gagtcaacac gattttgctc agaagtggca gcaaccaatt gtcgatctaa agaagtagct 105360ttactttgat cgatatccac aatctttctt ttttcattta gtatttctct cgcatcacct 105420tgatactttt ccttatcgat agtacctttt aaagcaactt caatggtatc atcaatttgt 105480ttgttatttt gatttctttt cgagatcaaa cgacaactat cttcattgtg accttggtgc 105540ttacaataat tgcaatacaa aggtagatta tcatatacaa tctcttgatt ttttttagat 105600aatactactt ttgtcctaat ttatgtgtca catttcattt tttagagtca aatagtttaa 105660gtttgatcga gaatttgccc atggaatctt caaatttttt aaaatgaaaa tatacatatt 105720tgtaaactat gtcaaaagta ttataagtca caataattga caattcaaaa tatttaaaag 105780atatatggaa aatttatgat caaagataga cttgtttgaa tctcgaaatc taaaaatatg 105840tcacataaaa tgagagcgga agtaattatt agttgagtga ccatatgaaa agggtaaata 105900ggcactgttt gtaagacaaa atggtatata taagggtaaa tagacactga cacactgttt 105960gtaagacaaa atggtacaaa attgctctta atgaaactta ttccaagagt tggtgaactt 106020gcaggaattt ttctactgaa gatggctcaa aatgaaatta aggaaatttt agatcacctg 106080agaaagatta agagtggagg taatctggat agcatcaaga ttgctcaaat tgaagtactt 106140gaaattgtgg taatgtatca taattttctt ttgcctgatt ccagagacat aatcatacag 106200aaggccaatt ggattgtgaa aatgcatcgg tgggtattag atagaattcc agttccagat 106260gaatgtaaaa ctaaccttaa tctaggaagg ttattttcac atttgttcaa attctttgaa 106320ggtaaaacca atttaagtta caattatgag ttgaaagatt ttgatctgtc gaaatatatg 106380gatttccttg gaaagactct

taatgatgta ctgatggttt tattggagga agaggttagg 106440tatgaccctc ctgaggaaaa ccttgaaatg cacgtattta taaagcaact gaaaattgtt 106500caaaagaaaa tgaaattttt gagacactta tatgacacag agataaatgg ttacatcaac 106560catgagaagc tggaatgttt ggagactcga attcagttca tggctaacaa tgtgggacaa 106620ctctgtattg ctgtttcagt tattcttttt tctgattttg tagatgatac aaatgagggt 106680gaggttgaca gatatgacac gtataagtat tatatcttcg ataaatctcc atatgtatta 106740tgcttgattg tgttagtgga gctggaaatg aagaagattt ttctcagtga actaaaggct 106800tccaagttta ctcaatcaag aactttcaaa gataagaaat taccaaaaga attttctcat 106860catctccata gtctgctgat gtatctcaga aacaaaaagc tcgagaactt tcctaataat 106920aaatcttctc aaaatattga tgtagcaata gagttcttgt tggttttcct tgatgctgat 106980gtggcaaatc atgtaattaa cggtaactgg ttgaatgagg ttatggaaaa ggttttagat 107040atagcgggtg atgttctata tgtaattcac aagcttctgc ctagctctat aaacaaagat 107100gacaatagca aaataagtct ttgctcgata cagatattgg agaaaaccaa agatctgaag 107160gcacaagtgg agacgtacta taaatccttc aaattcactc catctcagtt ttccactgtt 107220ggtggatgga gctttctgga ttctctgata cggaaactga atgagatatc gaaatctaaa 107280tctggtttag atttcctgat gaaacctctt ttggagaatt tggagaaaga gctatcagct 107340cttgcatcca ttttagagaa ggatctgtca tctttatcat ccattttcag agatgtcgcc 107400aaggtgcacc atgaacatga aattcttcaa gatgttcaca ggcgtactat caatttggca 107460tatgaagctg aagttgccat tgactctatt cttgctcact ataatgtttt ttggcatatt 107520ttttgctcac ttcctacaat attaaaagag atcaaacaaa ttaatgcgaa ggtgactgag 107580atatggtcag cagacgttgc tcttaaacct tgctatgtgg tagcaccatt taaacatctg 107640ccaactctag atagcaatcc agtaactgat gatgagatag tggattttgg gaattacaca 107700gaaaaaatga ttcagtatct gattagaggt aaaaatgagc tagacgtcat cccaattgta 107760ggcatggggg gacaagggaa aacgacaatt gctagaaagg tttacaatag tgacaacact 107820gtttctcatt ttgatgttcg agcatggtgc atcgtttccc aaacatataa ccggagaaag 107880ctattacaag agattttgag tcaagttacc ggttccaagg acaagggaga taaggatgac 107940atccttgctg atgagttgag gaaaagctta atgggcaaga gatatcttat tgtattggat 108000gatatgtggg attgtatggc atgggatgac ttaaggcttt cttttccaga tgttggaaat 108060agaagcagaa tcgtagtaac aactcgactt gagaaagtgg gtgagaaagt caagtactac 108120actgatcctt attttcttcc attcttcaca acagaagaga gttgcaaatt attgcagaaa 108180aaagtgtttc caaaggaaga ttgcccgctt gaactacaag atgtaagcca agcagttgca 108240gaaaaatgca aaggattgcc tctggtggtt gtcttggtag ctggaataat caaaaaaagg 108300aaaatggatg aatcttggtg gaatgaggtg aaagatgctc tctttgacta tcttgatcgt 108360gagtcagatg aatatagtct ggcgactatg caattgagtt ttgataactt accccatcgt 108420ttaaagcctt gtcttcttta tatggggatg tttccggagg atgcaagaat tccagtgtct 108480aaattagtaa gtttatggat agcggaagaa ttcgtggtga acattgaatc tgctgaagat 108540tacctgatgg atctcattag ctgtaacatg gtaatggttt caaagaaaga atataacggt 108600aaggttaaat actgtcaggt tcatgatgta gtgcttcact tttgcttgga gaagagtaga 108660gaagaaaagt ttatgttggc agtgaagggg cattatagcc agtttcaacc ttttgattgg 108720aatgaaagtc gagtgaactt ccatttgagt gaagagcatt ccaaatttgc atctctggga 108780tccaaaacac ggaagccttt ccaccaaccg ttgaggtcac tgataacgaa ccaaaaatct 108840tttcatggga ttcccttaag gtcttggatt cataagatgc ggcttcttaa ggtcttggat 108900ttgagttccc atgaagtgtg ttatttgttt tcagatacat tgaaaacact aaatcacctg 108960aagtaccttg cagtttcagc agagagattc tattttcatc cagaatcaca tctgccccat 109020cttgaaactt taattgtgaa gaataattgg acaaatacag tagtgttacc atcatcttta 109080tgggaaatgg aaaaattaag gcatgttgag attaggaaag ctgaatttga taagcagggg 109140ctctttgaag gatcctctaa attggaaaat ttgaggatat taaagaatat tgttaccttt 109200ccaattgata gggtggatgt gttatcaagg aggtgtccta atcttcaaca acttctcgtc 109260gaatttgatg gacattctgc agattatttt catctcacat tggagaatct tacccagctt 109320caaatacttg acctttcctt taagggaccc cacattgtat ctgggttaca attgccttca 109380actttaaaca agttggtact aagaaggact ggtataggaa acctgatttc cttcattgcg 109440ggactaccaa acctggagta tctccaatta cacaaccagg atcgtgttca aatcagagat 109500tggtgtcttg gagatatcac gttccataac cttaagttct tgaaattggc gtggttagaa 109560atctcaaggt ggaatgcctc ggaggaatcc tttcccctgc ttgaaacact tgttatggaa 109620gagtgtgacg acctcgagga gatccccctt agctttgcag atattccaac actgaaacaa 109680attaagttga ttaggtgtga gaacaaatct ctggaggatt cagctgtgag gattaagaaa 109740gatgttgaag agaatgaagg aaacgaccgt attgacctca ttatcaaagt aagtagcaac 109800aaactcctat gttctgtttt tgagtatcta tatgcatcta atgtgcaaac aatatgttta 109860atacaggata ggtgaggaaa tccaaagcag ctgtgagtaa agtatgtact tgtcacgaca 109920acatggtagt ctttcagttt cttttcttgt tcctttatgt ttttttcggg ataaaatgct 109980tgctttctct taattgctcc atgtcgatct caaagtaatg aggatgtgtt tgaatttgac 110040aattgctcag aaaactgcag caaaagcaca aacagcatgg accgaggtca ctcttagcaa 110100attgttgtaa ccatgctcat gctggagtct attagccaga acacccatcc ttgtgacact 110160actactgatt gctaagggat tttttattgt tcgacataga cgactcattc ctactgggaa 110220gggaaggatt atgccttgat tctctttttc cagtatgttt cagatgctct tccactgtga 110280tgccctatat aaatatcatc acttgctttt gtaacttggt ggttgttttc cccttttatt 110340ttctataact agtttcagga cacgtgcatt gcacatatat cccatgtgaa tttttatatg 110400atcccttcat cttttgtatc ttgatggctg tagcttgaaa gctccgacag tatagcgtat 110460gtgttcatca gtcacctcag agatgaaacc accatcagct aactcctcaa acttcacctt 110520cctaagtgat tttttttgtt attaacgtgc acaaaactct gttttccaac aatataattg 110580gaaactttcg aaagcattgt catatatact aacacgtcca tcagtcacga gtggtgatca 110640tgattcggtg gccttttctg tctctgggga aaacattcat gacggaatcc acaacgtctg 110700tcatccaagc atcatccaac atgataagac atttacctcc tttgtcaatg aattcacata 110760ttctgtagtg agtttgttga acactttcag aatactaaga aagaccccga atatcaggaa 110820atactcaaag gaaacctgag ggcctttaaa gatttttatt gcccatgtcg acttttaaag 110880tccaggcaag tgcaccacag ggataagtac aagcaaggag ggctaaacgt catctctagg 110940gaggtaaatc attggttcct tcagcaagtc atttgatcat tatttttgtt gaggataaga 111000actatatatt acccatatat ttgaccttac gtcttctatt aaacattttc ccatttcatt 111060tcaataaact tatttctagt ttcacctaat agtgatctca taaatatatc tttattggaa 111120tccaaatctg aaatctttgg tttacgaaat aggagtgcct aaaaatcctt gagtacgtga 111180aattacatag taaaatcact caacttagga gttatttgat gtgaggtgaa cgatataata 111240agttgttcgg aaatcatccc atttgattcc attatttttt ttaaaaaagg catatatgtg 111300gcaattttcg ctttgtgcag ataagataac tagggaataa tgaaaaaaaa tgagtaacac 111360aagacactag tacagtaaac atggggtcaa caagtctatg cattattttt cttctttaac 111420cttctatgtt acacatgcaa aactaatttt aagtatagaa atcatttact cgatatagat 111480attcgagaaa actaaagatc cgaaggtaaa agtgaagacg tactacaaat ccttaaagtt 111540cagtacatct caatttttca atattgttac tttgagtcgt ttgttggtct atatcactaa 111600taacaatatc aaattacatc agaaatgatc tatagtgaca attaattaac gacaatagat 111660taactgttga aaatatgtat ttttagaggc aataaacaat ctttgtaact gtctctaaag 111720ccaatagcga cattagatct aaggataatt aactaatgtc ggtaaaagct ttatcactat 111780ttactgccgc taatagatgt ttttgttgta gacttgtagt gatcatcatc tttatattcc 111840atgaataaaa aatttggttg gtaagagtaa ttattatatt tgatataaat ggtctaattt 111900tggtaaaaaa taataatatc aattataaat tttgtttaaa tatgaaataa atgattggta 111960gatataaaat aatttatttt taacaaaata taaacttgtt agttaatttt tgtatattaa 112020aaatgtgaaa tcatgatacg aaatttccaa atcaagattt ttggaaaatt tgagatttca 112080tctcattatt taaaatcatg agataaaatt atatgttcaa atgttgattt caattcatta 112140tttgatgaaa tcacatgttt aaacacctac ttaaataaag ctccgctaat gagccaattt 112200taaaaattga gttaggcctt aagtgtcatg tgatacaaat gaaatttcaa aagtcaatct 112260ttttttttat aaattctttt tgatatttaa gttatcaatt attgtgattt ataatatatt 112320taattttatg tattttatat aatatctcta tctctttatc ccaatttatg tgttatagat 112380gaaattttga gagttaacaa aatcttttta tatattttca aatattttga agatattaat 112440tattatgatt tataatatat ataaagtgat aaaagacaat tttttttaaa aaagaaaact 112500ttaaattgcc aaactaaccc tggctacaag catgcatgtt gaaacttgag aagttattca 112560aaattttatt tcttctatcc taatttatgt gacattaata aaattttaga gtgtcgataa 112620ttttttaata ctatttttaa atattagtaa tgaacaatca tattatcatt taaatttagc 112680cttagtactg aaatatgaaa tattaaattt tattctacat gatctcaaac acaataacaa 112740agaaagccta aaataactac aaatgaatac taaatatgtc tccaaaaaaa aaatgaatac 112800taaatattat tgatgggacg tgtgggtctg agtaagtgct aacatcaaca cttttatgcc 112860tttaaggcat tgaaaaaagc ctcatatttc aatatattta ccaatatgta ccggttgatt 112920tcttgtaaaa aggtacaaaa gaaggggcaa ataaagcttt gaaaaagtac tgcattatca 112980acaaaaggac acaataggaa ttagagggaa aaaatcatga aacgcacaat tcgcaaagca 113040aattgtataa gcattcacag caaagtataa agcagaatta gatagtaact tatcaaagta 113100tccttgtcta ggaagcaagc atgttgacga acagttatca ggaaacaaag tcatccacag 113160attgctaaca aaaaaaaaaa ttggataaaa cttcaaggag tattttatga gaaatatgag 113220aagtgaggag aatttgatca aagagagaaa acatagcaat tgttctttaa agttctacgg 113280aacaaaatga agaagtgtat gtttgaaagc cacgtggaat atggataatg ctctaaatta 113340cttatttgat aggaagacta atataccctt aggtgagaat ttatttccaa gaagctaaca 113400tgttgatact gagctgcttc tcatgcttct ataatagtag aataataatc taatcaaaca 113460taggaggaaa atttgggctc aaaaagtctt ttgcacattc tgtaattatt atttttatca 113520tttattgtaa aagagcattg taaagtactt gacaagtgac tctgttattg cttgataagt 113580cacctcatag gccccatttg catgtgtgat tgctttattc atttgtattt tcccctttaa 113640tttctaagca gcacctgcaa aatgttagca atatgtgttt aaaaatcaga attactgatt 113700tcaataaact ttgcagaatc acgaagtaac taaagtttaa taaaccagta agaataaaag 113760aacataaatt aattgacaaa actaaaatct gtaaaaaacg taccacaatc tgaaaaaaca 113820gaatcagaaa aagatcaagc ccactgaatg cacagtattc ccttaaggaa attattcccc 113880tctagtattc gaggtttgat ttggaatatg tccacccaga atagaatgat ctcaatcacc 113940agtgtattga tacccaaaac tctggtgtca gcgaaccact ttacagcagt aaagtacacg 114000aaaatttttt gtgcaaaaga agaagaagaa atcagaaaaa ttcattagga ataagtccga 114060ggaatcaatg tatttatagg gaagaggaac tggttccgaa aggttgtaat cctttcagaa 114120tccacacgac cattcatgaa agtttgcaac ctttcaaacg gtcatggctg tttctgaaag 114180ttgcaacctt ttagaacagt cacttccaac ggtgaaaaat tcaaataaaa tgggaaagat 114240ttaaattaaa cggatcgcgc gtggatccga gtcgggtcgg atcaattaac taattaattg 114300aatcggttaa ttaactaatt aaaacgttgg tcatttaatt taattaattc aataaataat 114360taacaaattt tgtccaaaaa ataatctctt gatcattttc cgaagctgaa gccgagcgag 114420cgacgacggc acgagggagg tccctctttc caaccctttt aacaattaat aggagtgttt 114480ctatatttaa actctcatat ttttctttcc accacagatg agggacaaat gccttttcaa 114540taaagcataa aaagactttt caagttctca actcttcaag ttcccaactt ttcaagttcc 114600tctcttctca tctttcctcc attttccatt aagtcttgct acatacccaa cacaaaattc 114660atttaaggaa atacctactg aagttatttt ctggttgaat ataacacctt tcattaatca 114720tcaactaaca aacataatat tttagcagta taaaatacta aaggtaaatt aaatatatat 114780ggaagaaaac ttcaatagtg actttagtga gatcaatact tggatgaccg tatagggaat 114840taactcatat tcgaaaacta aactgatgga tttgtttaca aagagtatta cctcaggaaa 114900tgttcgaatc tctcaagcaa taaagtggag ttcgattcaa aatgccaaat ggtgtttgaa 114960aagatagcgt tttaattcat ggcctttcat ttacttgaca agttacctca tggtccattt 115020gcctgcttga agaataacaa aaagtagagt tcttttgtac caatattgtc tcatgggtca 115080tattcatgat taaagtttag aaggtaacaa cttcaaaaat agcattaaat aaatgttcca 115140tttaatttgt tataactaac atttatgtct ctaaatatgc actatatatc tttcttttca 115200cttaaaagtt tgcgtttaga tatacggtat gattaaagtc atgatttcaa atcaatattt 115260aaacatgcgc ttttgaatta tgatttcaaa tcccaaattc ttcaaaagag aatgacttgg 115320aatttaaatt atattaaata taaaatttga tacataatta agtttatatt tttttttaaa 115380aaaaaagatt catcatttta aattttgcat aaaaaaactc atgtttgtcg tgaatagatt 115440gttcgtacaa tgcaaaatga ttgcatgaat agctagttac tgctattgga gatagtggat 115500ttttataaga ttatatgtat ttgataatca caaacatcta tttatgaaag gaacatgaag 115560atatatgatt cattaatgtg gcactttagg atattccgat atcttattta tgaatgatag 115620tttgctcatt tggtaagaat gcatattttt gagtaaattt gaagattttc acagcttgtt 115680ggtttttcat gtttatgcaa aaaatacaat ttaagaaatt aaaattgcat gtccaatcaa 115740aacttcaact ttatatcaat atgactccct accataattt catattgcct ctctagctaa 115800acgaacccca aatttgtaat tttttatgtt ttatcgtact tgttctatca ttcaaatctt 115860tcatggggaa agatcaacaa aatggattga gttctacaca ttacatgcat agtaaggttg 115920aacaagaaat tcattgttta gacggttgtt gaactatata taatttaggg ggattttagt 115980gtattttcct agaaaaagac aaataatata cctcctttgt cccattttat gtggcacatt 116040ttctttttaa gtccgtccca aaaagaatgt caacttacta taattagaaa taatttcact 116100tcaaatctta atgaaatgat ttataaccac aaaattcaaa agtctttttt aaaaatagtg 116160tattaagtca aacggtgcaa cataaaatga gatggaaaga gtagctgtat tattaagcta 116220cgttacagat accacctaaa acaacttgct agtactacaa ttcatctctt ccttcttcct 116280ttaaaccctc ctccatttca tttcaataaa cttcctccaa tttctttgtt ggaacataat 116340ccagatctga gaattataac tcttttttta cctcataata tttgtttttt ctcttgatga 116400aacctgcttt caatattaat tatcatctct tttgctcctt tttatgattg gatcacaaaa 116460ttgaagatat taaagaggta taagagaaga cagaattaca agttggtttc attgtaagtc 116520atcttcttca tgaatgactt ttttcctatg gatagaaagt tgatttctca aacagtccac 116580tcaactaatt gttactagta tattagctag aaagcccttt ttttttgttg taaaaatgtg 116640atttttcttc tatgcattgt ttaattttat acaagttaaa tgtttattta tgcacatcca 116700aagttagagg gcatgaatat caagttaaag aatatatgta tgtattatgc ctccaacaaa 116760tccttgaagt tctgtattcc atctcagttt ttcaacattg ttaattactt tgagtcgttt 116820gttttcaata tcattgataa ctatatcatc atgtttatat ttcataaata aaaaatttgg 116880ttggtaagag taatggttat atctgatata gatggttcaa aggttggtaa gaataataat 116940atcgattata aattttgttt atacatgaaa taattaagtg attgatagat ataaatgact 117000tttttcttat aaaatataaa gttatgggtc aatttttata tatgaaaaat gtgaaatcat 117060aatatgaaat ttttaaatca aattttggaa aattttgaat ttcacctcat gagctaaagt 117120tgcatgtcca aacattgact tcatctcatg atttcaattc acgagataaa atcgcatgtc 117180caaacaccta cttaaataat actccccccc cccctccttc cccccatgag ccaattttaa 117240aagttaaatt aagcctaagt attagttgat catcttacta aaattagttg tccattatta 117300gttgttcacc ttactaaatc agaaaagtat taattaaaat tttcttatat taccctttca 117360attaattttt taaagtattc acattcgctt ataaaatatc aagaaatttt ttaagggata 117420aattggtaaa attatttttt tatttataat ttcttaatat gcatgcgaaa gataatttta 117480ttaaagtaaa gactagactt cttttgtacc aatataacaa gtcgcctcat aggtcatatt 117540cataattaca gtttaggagg taaaaaaaat ctttttaaaa ggcatctcgg cgactgagaa 117600ccataaggtg agcgccaaaa cgctagcacc aactgcttcc acaaacatgc atgataaggg 117660ggtgtgggga caaccaggcc accactttat ttgaaatatg agccgctaaa atttatcccc 117720ttttgaatgt atttacccct atgaacctga tgcatacaaa acttcaaaag taatatacgt 117780tctattttag ttgatcattt ttcacacaca tattaataat cataaataga aagataattt 117840tgttaattca cccaaaaata attcgtagaa ttttttacaa ataaatgtga acactttcaa 117900aaagaataaa ttacaagggt aatacacgaa aaatttaatt aatgttttct tgatttagta 117960aggtggagtg atgaacaact aatatgagac aactattttt agtaagatgg tcaactaata 118020tgggacgaag ggaacaatac ataaatgcat gtttctttta atttgacttc aattgatttt 118080tatgtcttta actttgattg tacacaataa tatatcttgt ttttcacttg taagtttgcg 118140cttggacata taatatgaac tcatgatttt aaattccaaa ctcccccccc cccctcccca 118200aaaataaaac ataatttgga atttcaaatt atgatatcaa attttgtaaa atagaaaact 118260tgatccataa atttatattt tgtaaaaaaa aaataggact cgtgctcaac gtgaatagat 118320tgtccgtacc atgtgaaagg attatattaa ggaataacta gttgattaat tagtggatcg 118380ttataagatt aatcgcattt gaaagttatt tataatcaca aatatttatt caaggagata 118440tgtgattcat caatgcgttg ctttaagata tttttttatc ttgtttatga atgatgattt 118500actcatttga taagattgca tacgaattgg agaaattttg ataattttca caacatgtgg 118560cttttcatgt ttatgagaaa aaaatataac ttaagaaatt tcaattgcat atcaaacaaa 118620atttcatctt catattaata taatttcata tcacctgtcc aaattaaaca tgccccaaat 118680taataatttt ttactttatt gtactttatt caatttaaaa aataattcat ccgactactt 118740tttaaggggt acctgcaatc tcaaccagct agctgttttc tcatcatttc catagtctgt 118800tgatgtatct cagaaacaaa aagttcgaca actttcctaa ttatagcact gctcaaaata 118860tttgtgtagc aataatagag ttcttggttg ttttccttga agctgatgtg tcaaatcatg 118920ttattaatgg taactggttg aatgatgcta tggaaaaggt tggagctata gcgggtgatg 118980ttctatatgc aattcaaaag gttcttccta gatctataaa caaagatgac accagcaaaa 119040tgagtctttt ctccatacat atattaaaga aaactaaaga tctgaagaca caagtggaga 119100cgtactataa atcattaaaa ttcactccat ctcagttccc cactgttggt ggatggagct 119160ttctggattc tcttatacgg aaactgaatg agatgtcgga atctaaatct ggtttatatt 119220tcctgatgaa acctctcttg gggaatttgg agaaagagct atcagctttt acatccattt 119280tagagaagga tttgtcatct ttatcatcca ttttcagaaa tgtcgccaag gtacaccatg 119340agaatgaaat tcttcaagat cttcacaggc gtactatcaa tttggcatat gaagctgaag 119400ttgccattga ctctattctt gctcactata atgttttttg gcatattttt ttctcacttc 119460ctacaatctt aaaagagatc aagcaaatta atgtgcaggt gactgagatg tgttcaacag 119520acgttgctct taagccttgc tatgtggtag caccatttaa acacctgcca actcgacata 119580gcaatccaat gactgatgag gagatagtgg gttttgggaa tgatacagaa aaaatgattc 119640agtatctgat aagaggtaca aatgagctag acgtcatccc aattgtaggc atggggggac 119700aagggaaaac gacaattgct agaaagttgt acaatagtta caacattgtt tctcattttg 119760atgttcgagc atggtgcatc atttcccaaa catataaccg gagagatcta ttacaagata 119820tttgtagtca agttaccggt tccaaggaca agggggataa ggacaaggac aagggggaca 119880aggacaagga caaggacaag ggggataatg atgacatcct tgctgacgag ttgaggaaaa 119940tcttgatggg caagagatat ctcattgtat tggatgatat gtgggattgt atggcatggg 120000atgacttaag gctttgtttt ccagatgtta gaaatagaag cagaatagta gtaacaactc 120060gacttaagaa agtgagtgag caagacaagt accatactaa tccttattct cttccattcc 120120tcacaaaaga agagagttgc aaattgttgc agaaaaaagt gtttcaaaag gaagattgcc 120180cgcctgaact acaagatgtg agtcaagcag ttgcagaaaa atgcaaagga ctgcctctag 120240tgattgtctt ggtagctgga ataatcaaaa aaaggaaaat ggaagaatct tggtggaatg 120300aggtgaaaga tgctttattt gactgtcttg atcgtgagtc ggaagaatat agtctggtga 120360ccatgcagtt gagttttgat aacttaccca attgtttaaa gccttgcctt ctttatatgg 120420ggatgttttc agacgacgca agaattccag catctaaatt gataagttta tggattgctg 120480aaggattcgt ggagaacact gaatctgctg aagagtactt gatgaatctc attagcagta 120540acgtggttat ggtttcgaag aaagaatata acggtaagat caaatactgt caggttcatg 120600atgtagtgct tcacttttgc ttggagaaga gtagagaaga aaagtttatg ctagctgtga 120660agggaaatcg tagccaattt caaccttgtg attggaagga acttcgagtg agcttcaacc 120720aaacacggaa tccaaaacac agaaaccttg tgattggaag gatagtccaa gagcatttca 120780actttgcatc tctgagatcc aaaacatgga aaccttgtga ttggaaggaa agccaggtga 120840gcttcaacca aacaccgaat ccaaaactcg gaaaccttgt gattggaagg aaagtcaaag 120900agcattccaa gtttgcatct ctgggatcca aaacacggaa acctttccat caacagttga 120960tgtcactgat aacgaacgga gaatattttg atgggattcc cctctgtcag attcataaat 121020tgcgacttct taaggtcttg gatttgagtt cccatagagt gaattctttg tcattagcta 121080gtttcaaacc actaaatcac ctgaagtacc ttgcagtttt tgcaactaaa tttgattttc 121140atcccgaatc acatatgccg catcttgaaa ctttaaccgt gaataatgat tgggaaaata 121200tagtagtgtt accaacgtct ttttgggaaa tggaaaaatt aaggtatgtt gagatcgaat 121260atgctgaatt tgataagcaa ggggtcagtg aaggaacctc taaattggaa aatttgagga 121320tattaaagaa tatttttaga ttcccaatga tagtgtggat gtgttatcaa ggaggtgccc 121380taatctccaa caacttcaca tcggctttgg ggactataat tattctgcgg agtctttttg 121440tctcacattg aagaatctta

gccagcttca aaaacttcgc cttacctcta agtggcgccg 121500cactgtatct gggttacaat tgccttcaaa tttaaagatg ttggtactaa gtgggactga 121560tataggaaac cttattgcgg gactacgaag cctggagtat ctccaattac aaaatgtgta 121620ttttcctcct tcagaagagt ggtgccttgg agatatcacg ttccctaaac tcaaggtctt 121680gaaactggcg gcgtcacata ttttgaggtg ggatatctca gaggaatcat ttccccagct 121740tgaaacactt gttataagag ggtgtaagaa tctcgaggag atccccctta gctttgcaga 121800tattccaaca ctgaaacaga ttaagttgat tcgctgcaat aaatttctgg aggattcagc 121860tgagaggatt aagaaagatg ttgaagagaa tgaaggaaac gatcgtactg acctcattat 121920caaagtaagt agaaacaaac tcatatgttc tgtttttgag tatctacctg catctaactt 121980gacagcaata tgtataatac agtattatta gggtcatttg gcagctgtga atcaattatg 122040tgcctgtaat cacaacaatg gtaaatattg tttctgtttt tttcttgttc cttcttctaa 122100tttaatgttt gcttttgctt aattgctcca tgttgatctc aaactaacaa ggatgtattt 122160caatttgata attgcagcaa aagtacagcc aattgttgta gccgtgctca tgctcatgta 122220tattgtgcgc ctagtgccga catccctgtc ctttttctgt atgttttaga tgttctttta 122280ctgtgatttc ttgttgcctt ttataaacat catgcctttg ttatgtagct ttattatttt 122340acaacctgtc aaaacattgc ttcttgtttg tttttgtaat atagtagttg tttcccttca 122400tcttactcct atacaaggtg aattgaaaga tgagtattcc tgaatgaaag ctgttttgat 122460agcttagtac taaatagttt aggagcaatc cataacatac gtctcacttc ataaataagt 122520attgagtaca cgtacaaaag ctatctatgt tgtttggact cttcaaaaat gtcttccgag 122580tgtatattgg attgtccatt ttacattttt ggaggatccg acatgggcac aataatatct 122640ttggagagtt tgagcatcat aagaagccaa atagataaca cttgtcaaac ctctattcag 122700ctttctaggg aggacaatgg cagagtctcc caagttgacg acatgtatgc atgtctctca 122760cctttgacaa catcaccaga ggtatacgat tcgagttctt tcatgtcttc cgttgtcaat 122820tttacagata aggctcctat gttttcgttg aagttttcaa tcttcgatgc acctggtata 122880gggcatacat catctccttg atgaagaacc catgccaatg ctaattgtga aggggtgcaa 122940ttagattgat tttctcaaat actggcttta tgctcaaaat tctctggctt gaacctagag 123000aaattctaca aagcaaatca gcaaataata aattaatgtt gcagtcgaaa tagaaagatt 123060cattgatgaa accattacat agctatatat atatttcacg gagcaaaaag gttgtgccaa 123120aaaatttatc cgttaaattg tgcgttaaat cagtcgccag atgcatctca tgaaatgttc 123180aacgaaaggg cgagtgggaa gtttaggaga atttttcctg aacttggaat aacaactgca 123240attataacta tgcctcaatc acaaataagt tgcaatcgac tatatgaaat cttgatttct 123300ccacttaagt tcaactaacg tcatcatcat accaaataaa ataaaagcaa aaaataagtt 123360aaatatataa aacaatatta gaagttctta gaaaaagact aaatcctact ctccacggct 123420agatatctcc tatatatggt tctatgtctt ctattgtgcc atattttttt gctaagtatg 123480catttatcct tagaaatttg taagtatttc aaaattctac ctcgtctcct tttcaacacc 123540tccactcgcc ttagtttcac acctacaaac cagtgcatcc gtaggtcgac acaagacatg 123600tcacaccatc tcaagcgcac aatgctactt gcacttctag cgaatatgga tcattttaaa 123660ttttatttaa tattatatga tcgcatatcc atcttagcag ttggtcaaat tactgcatgc 123720ttggtaaaac cagattactc catgcttagt tagtgtcggc ctgtcgggca gtttaattca 123780aagacatcag catgtccata aaccagtaga atttccagat ttcaagaaga cttaagataa 123840ggacatccag ttgatgctct ctgtaaaaca tcaatgcaca aagaaataac aggatactaa 123900attcaaatgc aactacaaat atccagttga tgctcactgc acattcgtat aaatgtctat 123960taaacaggaa acacaaaagc agtcacagtt ttgatttatg tggtctaacc ttgcaaaagt 124020caccatcagg caagatttca atcaactttg cccctgctga gaagaatcct cgtccgagag 124080gactataggg gacaatccct atgctaaggt caaaaaaatc aaaaactgct aatcttgaac 124140tcaaatctaa aataaaaaat gtagaaagga aaatacttgt tattatttag atacaaaaat 124200ttcatacttt cttttaacag acacagtgtc ccagctagct tgcacgaacc tcaatcattc 124260caaggggtac ccgcaactct atccaccaaa tattagatag ctaggaagac atcacctaat 124320ttataccttt actatcattt gaacttgaaa cctcatggtt ctcgacccct atcatccaca 124380actagccaaa tacatatcac tcgtcaaacc cctactcagc tttccagaag gacaatggtg 124440acgcgcatct ctgaatttat ccaacaaaat aagatttacc agtgtcaatt ataagactct 124500aaataagtag aactgattta tttgcagtaa gaacaacaat gtgattaata aaggacaatg 124560tgaaagttca ggtagtcaat taattagttt tctctgtagc caactgtcct ctccttccac 124620acactttgat taatcattaa taatagatac taaccttcca attaattagt tgtctctctg 124680tagtatatca taagccatgt tatttcatta aataatgcaa ccattgactt tgaaagccaa 124740tgggaatgca tattaacctg tctaaaaatt aatatgataa acccaaaaat tattcattaa 124800ataatacaac cattgacttt tttgtaggat ttagatccag tcctttggct aaaccataat 124860tatacatcca ctaatcttgt tagagtatat gacacaaaca tttcctcatt gttccaagtt 124920aattatgctc atattaatct tttcttgtta gcttaatcct actccaatct ttatcaggta 124980tttttcaata aacatttttc taatctccct atgaattttt atcctttctt atatataagt 125040tatcaacaac tctttttcta tgattggatc agaacttgga gatacaaaaa ggcaaacgaa 125100gatcgattga tcgactactc gtcacgcttg aaattagtaa gatatattga atgttaactt 125160tctattgctc tccaaaatct cgaacttctt aaattgatac aacaactgag ttattaatcc 125220attttaccaa taaacttcat ggttccaaaa ctacaaatat tgtgaatatg gttttgatgt 125280atattaatgt agacatttat gattatcttg tatgaaacaa tatggcactt caaaacactt 125340gtgtttttat tccttatatg gtttagataa ttaaaaaatg atatcaccca taataattag 125400tttttgctcc gttgaaccag ttaaagaagt gttttatatt tgttttcctc tttaaatttc 125460tatttaaaaa aaaagtgatc aacagcagat ggctggttat atgtttttat atatacaaat 125520tgggggtagg gatttaaact taaggtgaaa attctgatat ccaaccaact gagcgactaa 125580tcgtgctatc cttctccaca cacatttttt tttattaatc attgggtatg cattagttat 125640ttctctgtac tagtaattaa tagggaaaaa ttcgctaaaa tatgtcatcc aactttgaga 125700aaaaacaaac ctcatttatg tcgtccatta atagttgggt tcaatcatgc ctttgtcatt 125760acataaatga cccattatgc cattattttt taatattcag attttgtggt tttgcaacac 125820catttttcca tatggcctct tattagaggt ccacgtcacc aaaattaaac tggtcaaaaa 125880aaaattcaaa tatgtcatgg aactgtgaca aacggttcta tgtcattcgt taattgttgg 125940atccaaccat atcacagccg ttaaatttga aattaaaatc caacccatta ataccagacc 126000cgacccaccc taacccattt cccttttatt tctcctacaa acgcttggat ccaaaatttc 126060tcccatttta atgatgaatt tatatgtcag tttcttgtca attgatcaat ttaagggtct 126120ggggagaaat aaaaagggaa taggttaggg tgggtcgagt gtgaatttat ggaccaggtt 126180ttaatttcaa atttaattaa attaggttgg tttattttgg cacgtggacc tctaataaga 126240ggccacgtga aaaaaaaaaa tttgcgaaat gatcaaacct gaccatttaa aagtagcatg 126300aatgggtccc ttctgtaacg acaatggaat agttgagccc aattattaac agattgcata 126360aataagacct tttttctaag tttgatggtg catttaaatt ttttttatca gtttaatttt 126420ggtgatgctg acctctaata agagaccacg tggaaagaac gattttgcta accatcaaac 126480cagctgcctg ttaaaaaata atggcacgaa tggaccgttt gtgtaaagca gtgacatgat 126540tgagcgcaac tattaacggg aggcataaaa aagtcttttc ccaaagtttg atggcaaatt 126600ttaactcttt tacattattg ttaactcaaa cacaatttaa agaattttta tgtgattttt 126660taatagttga tatctaggca cgtgcaacga tactagtact actatattat aatacctaag 126720tgttagtcaa agcaagtatt gtgaacataa aagctacgac aattgtatgg aatatgattc 126780ccttttataa gtgaggaaat tcattttctc ccttttagat gaaacttgtt tcaatttctc 126840gacttccatc acagtttaga cacacaatag atgagcttga gatctaaaaa taagcaattt 126900taagttgatg tttcattttc catttctata tgttaagtta gtcggtagaa taattataat 126960gaccatcttg tcagggccaa gttttcgtga tatgcatcgt ggagctcaaa agtgtagtga 127020acttatgttc tgtattgtca ctttttttaa aaacttattt gggctattcc tatgtgtgat 127080tcaactaaga tgcttgtcaa tccttttctt tgacctgttc ttatcggtcc accctccatc 127140attagcgtca cacatttgat attagttttt tttgtttaaa gaaaatgact attagttgag 127200tgaccatatg aaagttgagt gaaatgtgat tttatgagat aatatgtcac gccccgagcc 127260tacaccctgg gcgggaccgg cacccggaga ccattcctgg ccccaagcga acccttggcc 127320tggctttctt aactcagcgg aaacctaact caacagaata actcaatgcg atgcaatatt 127380gcaaaacaac ttaacataaa aagtatggcc ataaggcaac ccgagtctca aaataggatg 127440tttacatata tacatagata agagacttaa aactagctga ctgactgtct gtctgtctat 127500gaagcctcta aaaaaaaata ctgagatgga tgttgggaca gaccccgcaa tatcctaata 127560agacaaaact aagaacacaa agtaattgag tcctccggaa tgcaaggagg ctcaccactg 127620actctggagt gctcagctgg atcaacgacg tacaggatgc tgatccgggg tacctgaatc 127680tgcatcatca aacgatgcag gccaactggc atcaatacat ggaatgtacg agtatgcaag 127740atggaaaact aagcaacaaa ggctagaagg aaatctggaa gaaactgaac aacttacctg 127800gctcaactca acctgactga cttctttcaa tataaggcaa tttaaaacaa gtgcgatata 127860aagaaagact gcttaaaaca tgctataaac tctgtgtgta tacaaagata caataggcct 127920gaaatgtata tagaaataca atgaactgat gtatataaaa tacaataact gtagtgggag 127980tttctctaac cgacaaccat cacataagag ctatagtgat gatacagcga tcgacctcac 128040gctgccagag catcttatac ccggccaaag gtataagacc tgaactgcct aatggatcca 128100ctagtctaat ctgaaaagga ttcatctaaa aagtatgatc cttttctacc catggtggct 128160aacatggttc tatgggggct gtgggttctt tgaacgctcc cccaattcgg tgctcgatac 128220tactcccaaa atgtactggc tcttatgttt ttaaaacata tttattcctg ctgatatgag 128280ataattactc aaaaactagc ccgaaggctc ctttggaaat cttagtttcc aaccttgtct 128340aaatgtaaaa acatttattt aaaacttctt tgggaataca tagttcccca ataactttga 128400gaaaagaact caactttaaa ctcatgactt aacttgaaac tgagactctt actcgacttg 128460aaactctata ctcttttact tgacttctaa ctatctttcc ttgaatcgga actatgaatt 128520caagggattc gatcacatgt tgaggaggga tccttgaacg cttagacgta cttaggagtg 128580tcggaaacaa ctataaaaca taggtataat acttggaact tgcatgagaa agtgagaaaa 128640ggaatgggga aacttggcta aaacttcaga tttctggtga cacaggtgtg acttacggac 128700gccatcgacg gaccgtagat ggacttacgg tccgtcctgc aggtccgtag atcgcttcag 128760aaacttcccc agaattcatt tcagaaaatg actaagtgtt gacctacggt cctgacttac 128820ggtccgtagg tcaggtcacg gaccgtaggt cgtgaccgta gatcgaagcc ccaaaaaccc 128880aacttctgtt ccgattgacg gttgaccagt acggaccgtc aatcgatcta cggtccgtag 128940gtcaggcccg tagacgggaa tcatcagccc agaaatttct gtgaaataag gatgaatcaa 129000cttcacaagg gtcataactc tagaatcata attccctcaa catacaatag gtttcaactt 129060aaatactcga ccccaatcat gtctcacgaa gaacaataac ttcaacaaca aattccatct 129120tttctcaaat catagaataa acttggtgtg tgtgagggaa aggatcaacc cacacgaaaa 129180ctcacatacc ttgataggga tcacccccga cgaaaatcca caatgatctt gttgatctcc 129240tctttcttct cttcttcttc ttcttctcct tctccttctt ctcttcttca atctcaagaa 129300ccctaactct ttctctttca aaatgggaca aaaatgatcc aaagatcatt ctaatacaac 129360aatatgagct caattaaatg atttgtgaaa ggaccaaaat gcccttaaat ttccggacgg 129420atttcccttc caactgccca acttctacaa agcataactc gctcatacga actcggaatc 129480gagtaaactc agtggcgttg gaaagattgt tccaagggct tcgcaaccat aactggaact 129540actcctaaat catcctgggc taggagttac gactactcaa agttggccaa aaactcattg 129600atttccacac ttagccaatt ttttccagat tcgtcatttt ttccaaaaat gactatttcc 129660aaatttcaag ctccttctaa gccacttcaa attgtcggat gttacataat atgcactatt 129720tgaattagat gaaatggtac ataaaatgta ttatctttca taagcattga catagctaca 129780aacactagat attgaaagac gtgaataaaa tgttgttgtt aatgaaccta atccaagagt 129840tggtgaactt gcaggaagtt ttcaagagaa gatggctgaa aatgaaattg aggaaatgtt 129900agatcacctg agaaggatca agagtggagg taatctggat agcgtcaaga ttgatgaaat 129960tgagaggctt gaaatggcac taagagtttt gagaaccttt ataaagtatc atcatgttct 130020ttttcgtgat tccatagtca aacacaaaaa gaatgccaaa ttgactatgg caatgcttcg 130080ccgggtattg gatgggattc cagatgaatg taaaactaac cttaatctgg aaaggctaga 130140atcacatttg ttggaattct tggaacgtga agccatttta aataacgatt atgagttgaa 130200tgattttgat ctgtcgaaat atatggattg cctcggaaag aatctaaatg atgtactgat 130260gatttcactg gaaatggtta ggtttggcct tgaaatacac ggatttataa aggaactaaa 130320aattgttcaa aagaaactga gatttttgaa atacttatat gccacagaga taaatggtta 130380cgtcaaccat gagaagttgg aatgtttgga gactcaaatt caattcatgg ctaacaatgt 130440gggacaactt tgtcttttta ctttaggtta tgttgatgag gatgaggatg aggatgagga 130500tgaggatgat atcttgaata aacctcctta tttattattc ttgattgtgt tagtggagct 130560ggaaatgaag aagatttttc ttagtgaact aaaggcttca aagtttactc attcaagaac 130620tttcaaggac aagaaattac caaaaggaat ttcttttcat ctccacagtc tactgatgta 130680tatcagaaaa aaaaaagctc gagaactttc ctgataatat ctctgctcaa aatattgatg 130740tggcaataga gttcttgttg gttttccttg aggctgatgt gtcaaatcat gttattaatg 130800gtaactggtt gaatgaggtt atggaaaaag ttggagctat agcgggtgat gttctatatg 130860tgatccaaaa gcttcttcct agctctataa acaaagatga tactagaaaa ataagtcttt 130920actcgataca gatattggag aaaactaaag atctgaaggc acaagtggag acttactaca 130980aatccttaaa attcactcca tctcagttcc ctactgttgg tggattgaac tttctggatt 131040ctcttataag gaaactgaat gagatgtcga aatctgaatc tgatttaggt ttcttgatga 131100aacctctttt aggtaatttg gagaaagagc tatctactct tatatccatt ttagagaagg 131160agctgtcatc tttatcatca attttcagag atgtcgcaaa ggtgcaccat gaacataaaa 131220ttcctaaaga tcttcagaga cgtactatca atttggcata tgaagctgag gttgccattg 131280actctattct ttcacagtat aatgtttttt ggcatattct ttgctctctt cctacaatct 131340taaaagagat cgagcaaatt aatgcgaagg tgactgagat gtggtcagca ggcatcactc 131400ttaatccttg ctatgtggta gcaccattta aacacctgcc aactcgacat agcaatccag 131460tgactgatga ggagatagtg ggttttggga atgacacaga aaaaatgatc cagtatctga 131520ttagaggtac aaatgagcta gacatcatcc caattgtagg catgggggga caagggaaaa 131580cgacaattgc tagaaaggtg tacaatagtg acaacattgt ttctcatttt gatgttcgag 131640catggtgcat cgtttcccaa acatataacc gcagaacgcc attacaagag atttttagtc 131700aagttactgg ttccaaggaa aagggagata aggatgacat ccttgccgac atgttgagga 131760aaagtctaat ggtaaagaga tatctcattg tattggatga tatgtgggat tgtatggcat 131820gggatgactt aaggcattgt ttcccagacg ttggaaatag aagcagaata atagtaacaa 131880ctcgacttga agaattgggt aagcaagtaa agtaccgtac tgatcctcgg gtattctctt 131940ccattcctca caacagagag acgagttgca aattactggc agaaatttta aagtgttgtc 132000aaaaggaaga ttgtccgcct gaactacaag atgtgagtcg agcagttgca gaaaaatgca 132060aacgactgcc tctagtgatt gtcttggtag ctggaataat aaaaaaaaga aaatggaaga 132120atcttggtgg aatgaggtga aagattcttt atttgactat cttgattgtg attccgaaga 132180atatagtcgg gctactatgc agttgagttt tgataactta gttgattgtt taaagccttg 132240tcttctttat atgggaatgt ttccggagga tgcaagaatt ccagtgtcta aattgataag 132300tttatggata gcggaagact tcgtggtgaa cattgaatct gctgaagatt acttgatgga 132360tctcattagc agtaacgtgg taatggtttc aaagaaagaa tataacggta aggttaaata 132420ctgtcaggtt catgatgtag tgcttcactt ttgctcggaa aagagtagag aagaaaagtt 132480tctgcttgct gtgaagggaa atcttagcca gtttttacct tgtgatttga aggaaagtcg 132540agtgagcttc attttgagta aagagaattc caagtttgta tctctgggat tcaaaacaca 132600gaagcctttc caccaaccat taaggtcact gatgacaatc ggaaaatctt ctgatgagat 132660tcccttgagt tcttggattc ataaattgcg acttcttaag gtcttggatt tgagttccca 132720taaagtgtat tatttgtcgt cagctacatt gaaaccacta aatcacctga agtacctcgc 132780agtttggtca gagaaattct attttcatcc agaatcagat ctgccccatc ttgaaacttt 132840aattgtgaag acttggagta atatagtact gttaccagcg tcttttgggg aaatgggaaa 132900tttaaggcat gttgagatcg ttgaagctaa atttgataag caggggcttt ttgaaggatc 132960ctctaaattg gaaagtttga ggatattaaa gaatattgtt agctttccaa ttgatagggt 133020ggatgtgtta tcaaggaggt gtcctaatct tcaacaactt cacatcgaat ttcacggggg 133080tgatagtgat tctgcagagt ctttttgtct cacattggag aatcttaccc aacttcaaat 133140actttgcgtt tcctttgagc ggccccacat tgtatctggg ttacaattgc cttcaaattt 133200aaagaagttg gtactaagag ggactgatat aggaaacctg atttccttta ttgcgggact 133260accaagccta gagtatctca aattacaaga tccctatttt cctcaatcag aaaagtggtg 133320ccttggagat atcaagtttc ataaactcaa gttgttgaaa ctggtgaact taaagatctc 133380aaggtggaat acctcggagg aatcctttcc ccagctcgaa acactggtta taaaaaggtg 133440tgaccatctc aaggagatcc ctcttaactt tgcagatatt ccaacgctga aacagattaa 133500gttgattagg tgccagaacg aatcactgaa ggattcacct gcggagatta agaaagatgt 133560tgaagagaat gaaggaaacg accgtattga cctccttatc aaagtaagta gaaacaatct 133620cctatgtttt gtttttaagt atctacctgc atctaactgg acaacaatat gtataataca 133680gaattattag ggtcatctgg cagctgtgag agtcaatcat gtgcctgtaa tcacaacaat 133740ggtatatata gtttcttgtt ccttcttcta atttaatact tgctttaact taattgctcc 133800atgttgatct caaactaacg aggatgtatt tcaatttgat aattgttgca gccatgctca 133860tgctcaagta tattgtgcgc ctagagctga aattcctgcc ctttttctgt atgttttaga 133920tggtctttaa ctgtgatttc ttgttgcctt tgttatgcag ctttattatt ttacaacctg 133980tcaaaacatt gcttcttgtt tgttttttgt aatataatag ttgtttccct tcatcttaca 134040cctgcaaaat tcaataaagt atacaattga aagctggttg aatacaagtt tatatacctc 134100cctattatca atcaatagaa tactgaatga aagttgtttt gatagcttag tactaaataa 134160tttaggagca atccataaca tacgtctcac ttcataaata atcattgagt acatgtacaa 134220aagctatcta tgttgttccg actcttcaaa aatgtcacca agtttatatt ggattttcca 134280tagaggatcg acacatgtgc aacaatatct ttggagagtt tgagcatcag aagaagccaa 134340atagatatca cttgtcaaat ccctattcaa ctttctagtg tggacaatgg tggagtctct 134400gaatttatcc aagttgacga catgtataca tgtctctcac ctttgacaac atcaccagag 134460gtatacgatt cgagttcttt catgtattcc gctgtcaatt ttatggataa ggctcctaca 134520ttttcgttga agttttcaat cttcgtagtg cctggtatag ggcatgcatc atctccttga 134580tgaagaaccc aagccaatgc taactgtgaa ggggtgcatc atttccttgc agctgttgtc 134640tcgaaattca tttccaatta aagtatttca gaaattactt gttatgaaag tgtttatcat 134700aaattgaaga agttcgtaaa atgacatcaa taaaatcgta aaacaaagag aagcaaaaat 134760ggaagagtag catagactta acaaggtgtg tcgcacaaaa catggactga tataccaagt 134820acaagtagta cttttggata acaacttgac tctataaact acgatcaatg aaataggatt 134880tacctgaatc atataatgca actacttctt aaaaattaag agtgatatga gtacactaca 134940aaaatataac tttggaacaa agatgtcaaa ccgtgacttc aattggcaac tgagtatcga 135000tgcgatgaac ataatacata tgtcattacc aaagtgtcaa ttttctgctg aatgcttctc 135060aaagtgagag agtctttcca ttatatagag aaaataaaga ttacacatcc ctaaagatcc 135120gatccgccat tccctacccc taaagatcag ctatctatct ctacaaatct gctattgaat 135180ccctctaaat ctgccttgct atccctccaa atcagctatt ctatcacaat atttattcct 135240ttgattctac aacaaatgta tatgatcatg ctaatttata tcactgtata attacattaa 135300tatatacaat catttagttg tccatcatgc taacacaaag taagtagaaa caaactcata 135360tttgctgttt ttgagtatct aatatgcaaa cagtatctta attactagtc acgacaacat 135420ggtattcttt caggtttttt tactttttcc tttatgttct ttctttttca gataagatgc 135480ttgatttctc ttaattgctg catgtcgatc tcaaacgaac aaggatgtgt ttgaatttga 135540caattgctta gaaaactgca gcgaaagcac taacagcatg gaccgagttc actcttagca 135600aattgttgta atcatgctca agctggagtc tattagccaa aacacccatc cttgtgacac 135660tatactgatt actaaggttt tttttaggga aaatgcataa gtacaccccc ttcctatgcc 135720caaaatccct gagacacacc taacctttac taaggtccta ttacccccga acttatttta 135780tatataattt tctacccctt ttcggcctat gtggcactat ccttgaaaaa attgtcaaca 135840cgcgatgggc ccacaagata gtgccacgta ggctgaaaag gggtagaaaa ttatatataa 135900aataagttca agggggtaat aggaccttag taaaggttag gtgtgtctct gagatttcgg 135960gcataggtta ggggggtact tatgcatttt cccttttttt tattgttcga cttagacgat 136020tcattcctga tgggaaggga agattttttt gccttgttcc tttattttcc tatatgtttc 136080agatggtctt ccactgtgat ttcatgatgc cctctataaa tatcatcact tgcttttgta 136140acttggtggt tgttttcccc ttttattttc tataactagt ttcaggacgt gtgttgcaca 136200tgtactcccc agaaaatttt tcgaactaag actcaaaccg ttcttcatag tgagtgagat 136260ttttgcaagg aatttgaaag ttctcgagtg ttaaggtcac tatatgtagc accttaagtt 136320ccaagaagaa tcgaagagaa aagcattcaa gtcattccta agtttttctt aagttttggg 136380caacttcaaa tgactataaa ttttagtaca gaatgagtta ggagacccat aagatgttaa 136440attaaagccc ttcgagtcct ctttccaacg ccaccgagtt tgatcaattc cgagctctga 136500gtaaaaagtt atgacgattt

actaacgtcg cacaaatcta gtgaggacaa tggtggagtc 136560tctgaattta tccaagttga cgacatgtat gcatgtctct cacctttgac aacatcacca 136620gaggtacacg attcgagttc tttcatgtct tccattgtca attttacaga taaggctcct 136680atgttttcgt tgaagttttc aatcttcgta gtgcctgata tatatagggc atacatcatc 136740tccttgatga agaacccatg ccaatgctaa ttgtgaaggg gtgcaattag attgattttc 136800tcaaatactt gctttatgct caaaattctc tggcttgaac ctagggaaat tctacaaagt 136860aaatcagcaa aataataaat taatgttgct tttaatttat ctctcttctt atcattacta 136920caatttattg ctttttcatc tcccctcatc attactataa tttaattttt tttttaaatt 136980agatttttta ccctctatat tttaataatt tttttaaatg aaaaatctta tttactaccc 137040tctttattat cataactagt taacatgttc gcgcttcgcg cgatcataac aaatattttt 137100aagacaattt cttttataaa ttagcacata ctaaaaaaag aagaaatatg atcaatactt 137160tggtaaaagc tttaaaatgt ttatgataat tgtttcaatt tataagatac tattttcgta 137220ttattttgta ggggtgaaaa gaatatgttt aatattttga aaaaaaaaat gctaacttaa 137280ttttctgctt aattttttat ttgtcgtgga tcatcaaatg aaacgtaaat aatctttcct 137340cctcgttttt taaaagtagg aatgcttatt ttatataagg aactactgaa cttagaccct 137400tttagatata attcttcaat atgatttgtt taacaaaaat atacttgtta ggtacgtaat 137460ctttcataaa attctttatt tttaaaaaaa gattgtgcaa gttaaaatat gaaaaataac 137520aaaaagcatg tcaatagtaa tacaaaggaa agaacgacta actattttct aatcaaataa 137580aatataaata taaaaaatca taataattaa attgaaaagc aatcaatatt aatatttcaa 137640caaacaactc gatacaaaac atatcatcat ttttatttgt tttaattatc aaagaataat 137700tatacaaata ataaatagtg aatattttaa cttttgagga gttacaaaac tcactaaata 137760tataattatt tagagaaaat cacaatttaa ataatgacaa aaaaaggcaa agaatgggag 137820aaaaagaata agtaggactt ttagtatata agagttcctg ctatcgtttg tttttatatc 137880atatcgaaat tatttataat ttttttcgtc ttcacacaaa taataaaaaa agtttatctt 137940tatttacaca atatatttca ctaaaataat acttcaattc attttagtgt gtattttgta 138000tgttttgttg aggtcattta tttaattact aaaaagttag aaaatagaaa aatatatttc 138060attattttag tacaaacttt aaaaatctca agttttaata aataatactc actccctcta 138120tttcaaaata agtgaattgt tccaagttta agagagatgt tgaaaaattc ttccattttt 138180gccctcattt acaatgttaa ataattataa aagaaatgac cttttaaata caaaatgtgt 138240acataacgtc atcaggaaaa taaattattt attttaaagt tatttagagc gcatagataa 138300atgacttaat tttgagtaat tttaatatat gacgtaaatg aaagaaaaat ggaaagacgt 138360cttaaaaaaa gagagaaaga attaagtaaa aggaaattga ggagacatgt taatttagaa 138420aaaaatataa atagataaaa aaaattataa gtcatgagat ggatcaaccc tttaataaaa 138480agaacaacgc aacatagtag tatttcctat taattttaaa caaaattaga gtatgtcttg 138540ttaccaaaag cttacaacca caaataaaga aaaaaacata tgctcccatg aaaatgttct 138600acgtgtaaat cttgaatagc tgatgtgcaa atgatagact aaaatctaaa tctattatat 138660gttgggaaaa ctaaatgtag aggtgatgaa aagaatcgta gaaaggagtt gaggagatat 138720gtcattattt aagtagaaaa aagtgaataa actaaggttt ttgtaattta tgagatataa 138780ttagctgtaa taaatattat attttttttt agttatcaaa caaattaatt aatgaagaga 138840gtaaatcatt ttttgagttt cataatcaac ttaaacaaat atcaattttc tctaaaactt 138900aagaaaatat gttcattgta attagatatt ggcagaacaa acttggttca ttttgaaaaa 138960tttcactaaa ttagaagaat taaatgtttt gtgaaatttg aatccaacaa taatatatat 139020cgactaattt tacacaagtg ttaacaaata agaaacaaaa acttaatgga ggggaactca 139080tctccttttt agttaattca aaatccacaa aagaaaaaaa tttgttatat catctaaatt 139140agaatgaaaa aaagcagata atgctaaaca aaagatgata gaatcttgat tgagaaatta 139200tgtcatatta acatgttata tacataatat atatcgatat aaaatatgaa ttgtatatgc 139260aaaagacaaa agtttatata atgaaagtat caattaaagt tttctttaag caacaaaatt 139320ataatatgtg gaagatcatg ttgcccttga tacaaaaagt gtgctttgta atataatctt 139380gttcttgaag ggtacatatc ttgatggata aataattaat tgaaaaatta tataagcaac 139440aataagtgca tctatatatt tgtaaattat tgaaacttct actttttcaa atcttgatga 139500atctcccacc gtcattaaac tttcttcatt ttgatgactt gtagcaaatt aatttttgaa 139560tctcttttgg cgaatcttac accgccttca aactttcttc ttcatcttga taaacttttg 139620cttggcaatt gtaacacaag aagaaattct aaaacatata tctataatct tcataaaagc 139680tgaaaaacaa aaaaaatata ttaacaatat gaagttggag aaaatataaa tggacatgca 139740acaacaccat agatgattgt tatttataga tgagaattaa gtagaaggaa gttgaggaga 139800cgtattattt aatttgagaa ttcaaatagg tagaggtttt tttgtaactt tcatgtgata 139860taattatatg taatgaatat gattgatttt tttaattttt taatgattga ttaatgaaaa 139920aaatgaatgg aaaaactcaa aaaaggaaga aaaaagataa atagctttct agaccattga 139980gaggtgtcac atcaccttgt ttagatcttt attatatata tatagatttt tgtaattaac 140040taagtaatac aaccttttat ataaattaaa taataaaaat aaaatattaa aaaaatattt 140100tttcgtaatt gtaattagaa gaaggacaaa aaattaattt ttttttatta ataatagaaa 140160ttagagaggt atgaattacg aataattatt ccttgtaagt tggcatgtga agagtctttg 140220ttgtaaatgt tttttcatta atataatatt tattgtaata aattaaataa ttgactttta 140280caataaaata agtaatttaa ataaaaagaa aggccaaaaa aatgagaaaa aagataaata 140340catatgaaga tcatagagag gtgccacatc accttgtcta tgtttctcct ttatattata 140400ttaagttaag atttataatt attattgaag aaaaatttct tctttaaagt ttttttttta 140460tggatctata aaatcagtaa aaataataat tcaaatacct taattgtctc tctcctcaca 140520ttactacaaa tttttttctt aaactttttt tttttacctt ctattttaaa aaaaaaaaga 140580ttctaaaaat aataatatta ttcatttctc ttccttatta tcataattta ttattatgct 140640taattttgta actattctct tttactcttc taaaacgttg tagaatttat aattttaatt 140700ttaattcaaa tgtagtaatt tcacattttg tttgtcaaag taatatccta acttagattt 140760ttttttatcg tgatttacta aattcatatt tgatttacaa attgaaagaa aaggaattga 140820gaaactatga ttatcttaat gctttgaagg cacatgataa cttaaaaaga tgtgattatt 140880tttctcatct caaaataatt aatatatttt gtatgcatat gctataataa taaacctttt 140940ttttaaaaaa aaattataaa aaggattcaa ctttattgct attggcattc tgataaagtt 141000tgtttagaaa acttttctca aatttgttat atgcattttg ataaagtata tagtgcctca 141060aataattagt ttctttaaat actttatatt atttgtgtaa ttggggttgt acatctctca 141120atctattctt ttttttatat aaaaataata aaaaattatt tatctctctt ctttattacc 141180ctaattaatt attcttattc acaaaaaaac tcttttttct taactgttat tattcacaaa 141240atttctcttt ttttttaaaa aaaaaaatat attctcttga tccgtaaaaa taagatatct 141300cttgattttt aaatattttt atcctttata ttttatgtta aaaaaaactc aaaagaagtt 141360atcctttttc tccttttcat gatttttaaa gatttttctt atttatattt tatgtttaaa 141420aactcaaaaa aagttataaa tagtatctaa tttgatatat ttttaacata attatagtat 141480tcaccttata ttatgattat aataaatatg tttaccttat tgatctctct tcttaccttt 141540actataattt attgctttca tctcctcatc attacctata atttaatatt ttttcttaaa 141600ttagtctttt taccttctat ttttataaat ttttaaaaga gaaaatctta tttatctctc 141660ctccttatta tcataattta ctattattat tgaaatttgt tttcttcttt aatttttttt 141720tcttttattc tataaaataa atgaaataaa attcaaatat catatatgtc tctctcccca 141780atccccatat tactacaatt tatcgctttt tcttaaattt ctctctctaa tatatatttt 141840aaaaagattc taaaaaataa taatattatt catttctctt ccttattatc gtaatttatt 141900attatgcatg gaatttctat tttttctcaa ttttatttct ttttatctat aatatttgtc 141960aaaaaattat ctatttaacc aagaatcatt attatttttt tcctcttatt cttgactttt 142020aaaatatttt ttcttattta tgtgttatgt taaaaaaatt caaattcaaa aaaattataa 142080aaaatattat ctaatttact ttttaatata gttgtgctca catttataat atgatattac 142140atttcaaata ataataatat atatatttat atatacacac acacatacat atatttatat 142200aacttttttt tctatttaaa atgctatata ataaagaaga aagagagaga agtagttaaa 142260gaaaaataaa taaaaagtaa cggttttaca tttttttcct ttcatgtgtt tatttttatt 142320gtagttactt ccaaaaaaaa attctttcct ttttcttctt taccttcctt ttacttttta 142380ctcaattcac tactatacat cttcttcctt ttattttgct atttcctgcc tcatctcctt 142440atattttttt tctaattata tcacactaaa tttattcttt actattagaa aagattcact 142500actatcttct tccttttatc ttgctatttc ctgcctcatc tccttatttt ttttgtaatt 142560atatcacact aaatttattc tttactatta gaaaagaaaa tcaaaaaaat atttttctta 142620taacttttca tttgtgaaaa agaaaaagaa tagaattaga gaactaatac ttcatggtgg 142680catcattttt acggtttaca aattgaatgt tagaggacat tttgatgtct ttcgatcttt 142740ggtatattga attcctaaat aggatttcat tgtacaattt tgatagatct gttgatataa 142800ttcttgttgg cataaaatga ttaaatttgc acaggtatat tctaatgaat tcatttatat 142860aaacaaatca aatttttaat ttacctgaaa tagtaaatct tcttgtggaa tgattcttca 142920tttttctcaa tttacctcca tatttgaggt agtaaaactt ttcaaaaatt caatttacct 142980gacgtagtat atcttctatt gcaataattt ttttccattt tgtcaactta cttttagatt 143040atgtgctctc atgcgtaatt tcatttgtat aaacaaataa aaaaacttga tttacatgag 143100gtagtaaaac ttttttcttt ttgacaataa tttttgaatt ttgttggtta cttttagttt 143160atgtgctctc ctattttata atttataatt tttaattgat tgattgtact aagttcttga 143220caatcgttac aactcttaat tctccattac aacaataaaa atatcatttt tattaaaact 143280tttcattatc ctctttaaga aatatcattt atgttagatg tatttagtag attattatat 143340taagaaatga ctaaaagagt tcaaaaaatt gagaaaaaaa taaaaataaa atggtggcca 143400tataggcatg ccacatcaac ttttctagat tcagcttcat atatatatat agagtttatt 143460ggtgcatgca gcaactccca agccgttggt ggcttaagtg tccctttatt ttcattcttt 143520attcctccat tataccttgt aacatatgta actcctaagg ttattatctt cactatttag 143580tacccccatt atcttcctat ttattcctag gaagacttct tctactataa atagtggtga 143640tcttcatttg ttttacatat aagaaaatat agagtgcata aagtttgtta aaaaaagaga 143700gttcttatta gttgaaggga tgtgtttttt ttgtggagct ttggactaaa ctcttgtcca 143760tagttgttga gttatctttt gtgaataggt tgttgtatcc tggaggggac aagtaaagaa 143820ggactactgc tggaccagtg aaaacatttg ctgcgtgggc ttgaatctcc ttaaagagag 143880cgagatatcc gcacctcagc ttgaagagat ttatttcttc atttttattt tcaattgtaa 143940tcttgtaatt ctgttatttt gtaatttttc actaaggact ttaagttttc actaacaatg 144000aataattagt ttatgctctg ttgaaccaat taaagaagtg ttttattttt gttttcctct 144060ttaaatttat ttatttttaa aaagagtgat caacagcaga tggctggtta tatgtcttta 144120tagatacaaa tttggggtag ggagttgaac cctcataact gagcgattaa tcctgctatc 144180cttccacaca cactttttta gattaatcac tgggaatgca ttagttgttt ctctgtacta 144240ataattagta gagaaaaagc tcaaatatgt cattcaattt tgtgggaaaa aaaccttatt 144300taatagtttg gctcaataat gtcattgtct ttatataaat gacccattat gccattgatt 144360tttaacggtc agaattcatg gttttgaaac actatttttt ccatatggcc tcttattaga 144420ggtccatgtc accaaaatta aattgattga aaaataagtt caaatatgcc attaaactat 144480gacaagatat tcgtctatgt cattcattaa tagttggatt caaccatatt tctagtcaat 144540tgatcaatct aagggtttgg ggataaataa aaagggaaat aggttagggt gggtccagtg 144600tgatgttatg agtcaggttt taatttcaaa tttaattaaa ttaggtggtt tatttttgca 144660tgtggacctc taataagagg ccacatgaaa aaaataattt tgcgaaatca tcaaatttgg 144720ccgttaaaaa agtagcacga atgggtcctt tctatagcga caatggaatg gttgagccca 144780attattaaca aatggtatat atgaaccttt ttccaaagtt tgatggcata ttcgaacttt 144840tttgatcagt ttaattttag tgatgtggac ctgtaataag aggaaagaag agttttgcta 144900agcgtcaaac ctgtatgtta aaaaataatg gcacgaatga accctttctc taatgcagtg 144960gcatgattga gtcagccaac tattaacggg tggcataaac gagtcttttt tccaaagttc 145020gatggcatag ttgaactctt ttacattact gttaattctt tgtttttttt aaaaataaat 145080taatatgacc acatgggtca ggcttttatt gaattaataa aaacaactac aacttcctag 145140aaagctaaca gtaaagaaga aacaaaaaga aaaaataaat atctggaaag tcttatgggt 145200ctcatttctg cttaggattt gcaaggatgt ggatccatat tttgagagta tttgggatga 145260tgtactattt caagacgtct tcttcgttgt cggtgttgtt gtaggacatc agcctagttc 145320caaatctttg aacatcgatt gggaaatcca ctagcttctg ctaagctggt aactcctctt 145380gacttgtact gaacattttt aatcaccatt ttcagatgta taatgaccga ctttgttctt 145440gttgtatttc ctcttgatct agcccccatt tgttgcttgg tcttgtttag gactgtgtaa 145500tctttctagt ttctgattct gaggattgga atttgtgatt tatccgaatt caatatcttc 145560cgtcctttgg tggggagatc ttgaaaactg tggtaatgga ctgggttatc ttgttcaagt 145620gcaatgcttg taatatagtc acctaactga tttgcttctc tgtatgtgtg tgtaatttcc 145680atctgtagtt gttgcattgt attctttatt tcttccacct gttgtactaa ttcccatggc 145740actttccaca cttcctggat gatgttcttt agtattaaag aatctcatgt ttctattctt 145800gcattatgta tatctcttga taaacaacaa tgcactgcct tttgtgctac tatggtttct 145860acaatcatat tggtgttttc ccctatttgt cctgcctctg catacaatag atctcctcta 145920tcattcctta ggcagaaccc gtaagagctc aatcctggat tccctttgct tgctccatca 145980gtatttatct ttattctgcc tgtttctggt ttcttccaat gtgtcaaaca atgatatagt 146040cttggtctgt aattacttag cacttgtatc attgatggcc aatcttttgg aacagaggag 146100ataccattct attatatgta gtatctcctc catgcttcct ataattcctt ctcttccata 146160atgattgctg ggatggcttt gaaaatgctg tgggggttga ttagttgtcc accacttacg 146220tatgacttgt tgcagttgca atccttcaat gttataacct gcacaagaag aaaactgtct 146280ccatagctta gtagctattg gagatgttag aaaaatatgt gacatggttt ctattttatg 146340atcctcacaa caccaacatc ttgataccac actaattttc atccttttta gattatcgtc 146400agtaggtatt cttcctttcc atgctctcca taaaaagaaa ttaatcttta taggcaaccc 146460tttagtccat atatagtcct gtattttctc cttcttccgt cttaattcat tccatgctga 146520tttgactgtg aactttccat ttgaatttcc catccaccat gatgtgtcat tatctacatt 146580gactagttgt gggctgatgt tatcaagtat atgctgcacc atatctactg ataagacttc 146640ctgtagtttc tgcacattcc aatagccatt tgtaatgaat tgtttcactt ctacctcctc 146700atccaataca ttttcattgt ccacgaaatg aaggcccctg cttagtccaa ttatcaaacc 146760agaagctaga atttccagcc ttaagttgcc accaaatcaa atgttctagt tcttctctaa 146820cttccaccat ctttttccaa acaggtgaac taccttttgc ttgagctatg acagggtgaa 146880gtttcttgtt gtatttgttt accatatcag cactctatag tgatctagtt gtccttagat 146940tccaccatag ttttccaaac aatgcctttg atgtatcatg tagtgatcta aaccctaagc 147000ctccttcaat tctaggtaaa cacatagctt cccaggtaac ccaatgtttt cttttcggac 147060ttgatgtatt accccagaag aaagttgcaa aatttttatg aatatgttct ataactcttt 147120tagggggatt catggctgat aggagatata caggtatcga ctgcaaaaca tgtgatatca 147180aaatatatct tcctccaaaa tataagagtt tattttgcca agacatcatc cttttcataa 147240ctttcttcac cagatcttca aaaaatacta tcttcttcct accatagaaa atcaggcatc 147300caaggtaagt aaaaggaaaa gtcccctttc tcatgccagt ttttcttcta tcttctttaa 147360cattactgtt aactcaaaca caattttaaa gaatttttat gtgatttttt aatagttcat 147420actcatcatc atggggtgca catacaacac gtgtgcctaa atactagtat attataatac 147480ctaagtgtta gttaaagcaa gtattgtgaa cataaaagct acaacaattg taaagaaaat 147540gattcccttt tataagtgag gaaaaaaaat ttcccccttt tagatgaaac ttgttttaat 147600ttctcgactt gtatcacagt ttagacacac attagatgct catgagatct aaaaataagc 147660aatgataatg accatcttgt cagagccaag cttttgggat atgcctcgtc gagctcaaaa 147720gtgtagtgaa cttgtgttat gtatttgtaa cttttttttt actttcttct cctatttggg 147780ttattcctat gtgtaatttc aactaagatt cttgccagcc taaattcttg tcaatccttt 147840tctttgacct gctcttatcg gtccgccctc catcattagc atcacacatt gatattagtc 147900ttcttttgtc taaagaaaat gactagtagt tgagtgacca tatgaaagtg agtgaaatgt 147960gattatctgt gataatatgc accatttgtt agatgaaaat ggtacataaa atatactatc 148020tttctcaagc atcgacatat atagctacaa acactaggta tgaaagacat gaataaaaat 148080gttgttaaat aaacctaatt ccaagagttg gtgaacttgc aggaattttt caagagaaga 148140tggctgaaaa tgaaattgag gaaatgttaa atcacctaag aaggatcaag agtggaggta 148200atctggatag cgccaagatt gatgaaatta agggacttga aatgacgcta agagttttga 148260gaaccgttat aaagtatcat catgttcttt ttcgtgattc ctttgtcaaa cacaaaaaga 148320atgccaaatc gactatggca atgcttcacc aggtattgta tgggattcca gatgaatgta 148380aaactaacct taatctggaa aggctagaat cacatttgtt ggaattcgtg gaacgtgata 148440ccattttaaa taataattat gagttgaatg atcgtgatct gtcagaatgt atggattgcc 148500tcgaaaagaa tctaaatgat gtactgatac tcttcctgga aagtgctagg tctgaccctc 148560ctgaagaaaa ccatgaaata cacagatttt taaaggaact gaaagttgtt caaaagaaac 148620tgagattttt gacatattta tatgccacag agataaatgg ttacgtcaac catgagaagt 148680tggaatgttt ggatactcga attcagttca tggctaacaa tgtgggacat ttttgtcttg 148740ctttttctga tgttgtaaat gatattgatg actatgagga tgaggatgtg tataatgata 148800tcttcaatag acctccttat ctattagtct tgattgtgtt agtggagctg gaaatgaaga 148860agatttttct caatgaacta aaggcctcaa agtttactca ttcaagaact ttcaaggaca 148920agaaattacc gaaaggattt tctcatcatc tccacaaact gctgatgtat ctcagaaaag 148980aaaagctcga gaattttcct gatgatgtct ctgctcaaaa tattgatgtg gcaatagagt 149040tcttgttggt tttccttgat gctgatttgt caaatcatgt tattaatggt aactggttga 149100aggaggttat ggaaaaagtt gtaactatag cgggtgatgt tctatatgtg atccaaaagc 149160ttcttcctag ctctataaac agagatgaga ctagcaaaat aagtcttttc tcgttatgga 149220tattggaaaa aactaaagat gtgaaggcac aagtggagac ttactacaaa tccttaaaat 149280tcactccatc tcagttttcc acctttagtg gattgagctt tctggattct ctttcaagga 149340aacttaatga gatgacaaaa tctaaatctg gtttagattt tctgatgaaa cctcttttag 149400gtaatttgga gaaagagcta tcatctctta catccatttt agagaaggag ctgtcatcca 149460ttttcacaga tgtcacaaag gtgcaccatg aacataaaat tcctaaagtt cttcatagac 149520ataccatcag tttggcatat gaatctgagg ttgccattga ctctattctt tctcagtata 149580atgttttttt gcatattttt tgctcacttc cttcaatctt aaaagagatc aagcaaatta 149640atgcggagtt gactgagatg tggtcagcag acgttgctct taagccttgc tatgtggtag 149700caccatttaa acacctgcaa actcgacata gcaatccagt gactgatgag gagatagtgg 149760gttttgggaa tgacacagaa aaaatgattc agtatctgat tagaggtaca aatgagctag 149820acgtcgtccc aattgtaggc atggggggac aagggaaaac gacaattgct agaaaggtgt 149880acaatagtga caacattgtt tctcattttg atgttcgagc atggtgcatc atttcccaaa 149940catataaccg gagaacgcta ttacaagaga tttttagtca agttagcggt tccaaggaca 150000agggggataa ggatgacatc cttgctgacg agttgaggaa aatcttaatg ggcaaaagat 150060atctcattgt attggatgat atgtgggatt gtacggcatg ggatgattta aggttttgtt 150120ttccagacgt tggaaataga agcagaatag tagtaacaac tcgacttgag aaagtgggtg 150180agcaagtcaa gtaccatact gatccttatt ctcttccatt cctcacaaca gaagagagtt 150240gccaattgtt gcagagaaaa gtatttcagc aggaaggttg cccacctgaa ctacaatatg 150300tgagtctaga aattgcaaaa aaatgcaaag gattgcctct tgtggttgtc ttggtagcag 150360gagtaatcaa aaaaagaaaa tcggaagaat cttggtggaa tgaggttaag gatgctttat 150420ttgactatct tgatagtgag tcagaagaat atacttgcgc gactatgcag ttgagttttg 150480ataacttagc agattgttta aagccttgtc ttctttatat ggggatgttt caggaggatg 150540caataattcc agtatctaaa ttaataagtc tatgggttgc agaaggattc gtgcaaaaca 150600gtgaatctgc tgaatattac ttgatggatc tcattaacag taacgtggta atggtttcaa 150660agagtagtta taatggaaaa gtgaaacact gtcaggttca tgatgtagtg catcactttt 150720gcttggagga gagtagaaaa gaaaagttta tgctggcagt gaaggggaat gttgtccagt 150780ttcaaccttt ggattggaat ggaagtagag tgagctttag tttcagtgaa aagctttcca 150840agtctacatc tctgagatcc aaaacacaga agcctttcca ccaacacttg aggtcactga 150900taatcagaga atattatgat gggtttccct ttaggtctag gattcataca ttgcgacttc 150960ttaagatctt ggatttgagt tccaataaag tgagttattt gtcgacagct acattgaaac 151020cactgaatca cctgaagtac cttgcagttt tggcaaacgt attctatttc gatccagaat 151080catgtctgcc ccatcttgaa actttaatgg tgaatagtga taatttggat tatatagtac 151140tgttaccagc gtctttttgg gaaatggaaa aattaaggca tgttgatatt tttagtgctg 151200aatttgattt ggaagaggat aagcaggggc tatcctctaa attggaaaat ttgaggatat 151260taaagaaaat tcttagattt ccaattgata ggatggatgt gttatcaagg aggtgtccta 151320atcttcaaca acttcacatc gaattttacg ggggtgatag tgattctgca gagtcttttt 151380gtctctcatt tgagaatctt acccagcttc aatatctttt cctttacatt gagaggccca 151440acattgtatc tgggttacaa ttgccttcaa atttaaagaa gttggtacta tgtgagactg 151500atatagaaaa cctaggttcc ttcattccgg gactaccaag cctggagtat ctccaattat 151560tggacccgga taaatttgtt

caaaacagag attggtgcct tggagatatc acgttccata 151620accttaagtg cttgaaactg tcgcgcttag atatctcaag gtgggatgcc tcggaagaat 151680cctttcccca gctcgaaaca cttgtcataa aaagttgtca ccacctcaag gagatccccc 151740ttagctttgc agatattcca acactgaagc agattaagtt gattaggtgc gagaacgaat 151800ctctgaagga ttcagctgcg gagattaaga aagatgttga agagaatgaa ggaaacgacc 151860gtattgacct cattatcaaa gtaagtagaa acaaactcct aaatatgttc tgtttttgag 151920tatctaatgt tcaaacaata tgttcaatac aggattgctg aatcaagtat gtgcctgtaa 151980ccacaacaat ggtattgttt cagttctttt taaattgatg cttgttttcc cttaattgct 152040cttaaactca cgaggatttg tttgaatttg actactgcag caagttcatg acggcttgcg 152100tccacacatt atcccgcaca atgaaacgtt tgccttctct gtcaatggat tcacatacta 152160tttttggcaa ttccttcaca tccatgtctt gatattctga agtgagcaag tttcttcaag 152220tcttttagag tactaagaaa gaccccattt agtttgtatt acgggcctac gttcaggaaa 152280aactcaaggg aaacctgaac atctttagag attttttggt tggatgtgat cttgaaactg 152340actctttctt ttgaaatatt gcaatttgtg gcagctattg aaatattctt tttttcacta 152400ctgccccacg tattgtcctt ttttgttctt tcatttactt gacatttgga gactagattg 152460tgttacgacc caaattccaa gagccgttac aaaaaaacat aatagatgtt cacactgatg 152520ctttgcgcta ccaaatatat gtgtttttac catcaactcc aaacgtatcg ttcccttagt 152580ctataacaat ggcatcttca cattctttac tagatttact gtgtcttaat taaaattatt 152640taaatatctt tgtatattct aatttaatca gacatcggcc gggcccacgg agaagacttg 152700gggtaaaaag aaaaaagtgt atgtgttgga aagttgatgg agtcttttca ccttttccct 152760tagtgctctc tgctttctcc ttttctcttc aataataatg cagaactcat aaagaaagat 152820tatgtgtatt tgaaattttt ttaatcacaa acattttttt tataaaagga acatgaatta 152880ataaagcact gattgttgtt tatctaccat taattaataa attaataatg caataattca 152940gaactcatac aatacacatt ccagtctgtt cgagccattg ccaactccaa ttattgtttg 153000atctcctcac ccatctctct gtactagaca acaccttgtg ccatatagga ttcggatcta 153060cgatcaatca gttaacaacc gatcgatcta ccactaagct actgaggaac aacaggaaat 153120tcgataagtt aagattgagg tgagttccat tgattcataa tttattttca tacttcaagg 153180aattccgtat cctctgaata tatatacccc ctcttcctaa tttaagtgtc ttagtttgac 153240gaggaattca atacacaagt cataatgaat aaagctaaca aattcttttc cgttccacca 153300agtctatcct acagccaagt aaacgaaaga aggaatgaaa aaggagaaac aaattataca 153360agtcacgagt acagtagact cgtattggta tataaaactt ttagtttttt agattgagtt 153420atatctaggt gtcatatttt tctaaggtat catagcaaga tccattctcg tttggacaca 153480tgatccatgc tccagttggg tctgaatata aacaggggtg ttatagtccc acattgattg 153540ggaaacagag tggtaatttg cttatatgta cttaaataat cattccctaa tgagttacac 153600tttaggggtt gagtttcgct caatgtcata tctctagaat catttaaata gtttttctat 153660agggaaacat gttttcaata ttaattataa tcatatttgt tctttttaat gattggatca 153720gaaaattgaa gatactaaag atgcataaga gaagaaaaaa ttacaagttg gtttcactta 153780ttaactaact gtataagtca tcttcttcat gaatgatttt tttcctataa tttttttttc 153840aaatggtcca ttcaaaagaa ttagttaagt gattttatga gataataatt attagttaag 153900tgaccatata aaaaaagata aatatacacc atttgttaga tgaaatgata catgagatgt 153960ataatctttc acaagcatta agatattgaa agacatgaat aaaaatgttg ctcttaatga 154020tcctaattcc aggagttgaa taacttgcag gaatttgtaa acagaaaatg gctcaaaatg 154080aaattgagga aatgttagct aacctacgaa ggatgaagag taacggttct cagagtagcc 154140tcaggattaa tcgaattgag aaacttgaaa tggtgctaag agttttaaga acctttataa 154200agtgtcattg tgttcttttt tctgattcct cagtcaaact cacaaagaat gccaaatcga 154260ttgtaagaat gcttcgaagg gtattccaag ggacttcata tatcaaagta gataagtgta 154320gttatgagtt ggttagggaa aggcaagtac cacatttgtt ggaattcttt gaaggtaata 154380ccaatataag ttacaattat gagttgaatg attttgatct gtcagaatgt atggattgcc 154440ttggaaagaa tctaaatgat atgataatga tgttcttgga aagggttagg tttgaccctc 154500ctgaaaaaaa ccctgcaata cacagattta taatgcaact gaaaattgtt cataagaaaa 154560tgaaattttt gagatactta tatgccacag agataaatag ttacgttgac gatgagaagc 154620tggaatgttt ggagactcga attcagttca tggctagcaa tgtgggacag ctttgtcttt 154680ctgtttcagt taacgttgat gctgattttt tatataatac acataacggt gaggttgaac 154740catatgggtg tgatatcttg aataaacctt cttatctatt atgcttgatt gtgttagtgg 154800agctggaaat gaagaagatt tttctcaatg aactaaaggc ttcaaagttt attcaatcaa 154860aaacattcaa ggacaagaaa tttccaaaag aattttcaca tcatctccac aggctgctga 154920tgtatctcag aaacaaaaag ctcgagaatt ttcctgataa tatctctgct caaaatattg 154980atgtggcaat agagttcttg ttggttttcc ttgatgctga tttgtcaaat catgttatta 155040atggtaactg gttgaaggag gttatggaaa aagttggagc tatagcgggt gatgttctat 155100atgtaatcca aaagcttctt cctagctcta taaacagaga tgagactagc aaaataagtc 155160ttttctcgtt atggatattg gaaaaaacta aagatgtgaa ggcacaagtg gagacttact 155220acaaatcctt aaaattcact ccatctcagt tttccacctt tagtggattg agctttctgg 155280attctctttc aaggaaactt aatgagatga caaaatctaa atctggttta gattttctga 155340tgaaacctct tttaggtaat ttggagaaag agctatcatc tcttacatcc attttagaga 155400aggagctgtc atccattttc acagatgtca caaaggtgca ccatgaacat aaaattccta 155460aagttcttca tagacatacc atcagtttgg catatgaatc tgaggttgcc attgactcta 155520ttctttctca gtataatgtt tttttgcata ttttttgctc acttccttca atcttaaaag 155580agatcaagca aattaatgcg gagttgactg agatgtggtc agcagacgtt gctcttaagc 155640cttgctatgt ggtagcacca tttaaacacc tgcaaactcg acatagcaat ccagtgactg 155700atgaggagat agtgggtttt gggaatgaca cagaaaaaat gattcagtat ctgattagag 155760gtacaaatga gctagacgtc gtcccaattg taggcatggg gggacaaggg aaaacgacaa 155820ttgctagaaa ggtgtacaat agtgacaaca ttgtttctca ttttgatgtt cgagcatggt 155880gcatcatttc ccaaacatat aaccggagaa cgctattaca agagattttt agtcaagtta 155940gcggttccaa ggacaaggga gacaaggatg acatccttgc tgacaagttg aggaaaatct 156000taatgggcaa aagatatctc attgtattgg atgatatgtg ggattgtacg gcatgggatg 156060atttaaggtt ttgttttcca gacgttggaa atagaagcag aatagtagta acaacacgac 156120ttgagaaagt gggtgagcaa gttaagtacc atactgatcc ttattctctt ccattcctca 156180caacagaaga gagttgccaa ttgttgcaga gaaaagtatt tcagcaggaa ggttgcccac 156240ctgaactaca atatgtgagt ctagaaattg caaaaaaatg caaaggattg cctcttgtgg 156300ttgtcttggt agcaggaata atcaaaaaaa gaaaatcgga agaatcttgg tggaatgagg 156360ttaaggatgc tttatttgac tctcttgata gtgtgtcgga agaatatagt cgtgcgacta 156420tgcagttgag ttttgataac ttagcagatt gtttaaagcc ttgtcttctt tatatgggga 156480tgtttccaga ggatgtgaga attcgagtgt ctaaattgat aagtctatgg gttgcggaag 156540gattcgtgca aaacagtgaa tctgctgaag attacttgat ggatctcatt aacagtaacg 156600tggtaatggt ttcaaagaga agttataatg gaaaagtgaa atactgtcag gttcatgatg 156660tagtgcatca cttttgcttg gaggagagta gaaaagaaaa gtttatgctg gcagtgaagg 156720ggaatgttgt ccagtttcaa cctttggatt ggaagggaag tcgagtgagc ttcagtttca 156780gtgaaaagct ttccaagtct acatctctga gatccaaaac acagaagcct ttccaccaac 156840acttgaggtc actgatagga gaatattttg atgggtttcc cttaaggtct tggattcata 156900aattgcaact tcttaaggtc ttggatttgc gttccgatgc agtgagttat ttgtcaacag 156960ctacattgaa accactaaat cacctgaagt accttgcagt tgtggcaaag aaattctatt 157020ttgatccaga atcacgtctg ccccatcttg aaactttaat ggtgcataat tattgggatg 157080atacagtact gttaccagcg tctttttggg aaatggaaaa attaaggcat gttgatattt 157140ggagagctga atttgatttg gaagaggata agcatgggct atcctctaaa ttggaaaaat 157200tgaggatatt aaagaacatt cttagatttc caattgatag gatggatgtg ttatcaagga 157260ggtgtcctaa tcttcaacaa cttcacatcg aattttacgg gggtgatagt gattctgcag 157320agtctttttg tctcacattg gagaatctta cccagcttca aatacctcac ctttcctttg 157380gggggcccca cattgtctct gggttacgat tgccttcaaa tttaaacaag ttggtactaa 157440gtgggactcc tataaaaaat ctgatttcct tcattgcggg actaccaagc ctggagtatc 157500tccaattact agatatgtat gttcctcaat cagaagtgtg gtgtcttaga gatatcacat 157560tccataaact taagttgttg aaactggggt ggtcagatat ctcaaggtgg gatgcctcgg 157620aggaatcatt tcccctgctt gaaacacttg ttataaaaaa gtgtagtaac ctagaggaga 157680tccctcttag ctttgcagat attctaacac tgaagcagat taagttgatt tggtgcgaga 157740agaaatctct ggaggcttca gctgtgagga ttaagaaaga tgttgaagag aatgagggaa 157800atgaccgtat tgacctcatt atcaaagtaa gtagaaacaa actcctaaat atgttctgtt 157860tttgagtatc taatgttcaa actatatgtt caatacagga ttggtgaatc aagtatgtgc 157920ctgtaaccac aacaatggta ttgtttcagt ttgccttttt taattgatgc ttgttttccc 157980ttaattgctc ttaaactcac gaggattcgt ttgaatttga ctactgcagc aagttcatga 158040cggctagcgt ccacacataa tccagcacaa tgaaacattt gccttctctg tcaatagatt 158100cacatactat ttttggcagt tccttcacat ccatgtcttg atattctgaa gtgagcaagt 158160ttcttcatgt cttttagagt actaagaaag accccattta gtttgtagta cgggcctacg 158220ttcatgaaaa actcaaagga aacctcaaca tcgttggatg tgataattca aattgactct 158280ttcttttcgt tattattggt tttattagtc gttgtcttat aacagactct ctagctaaaa 158340tcggagaaac aggaaacttt cttcactgag gttgaggttg tctctttgca tgacaagtta 158400gagttgcaaa attaactcaa ctacaaaaaa aatttcttgt tgaatataag caaacctcac 158460aaatttacaa attttcgcag catagaaaac tcgaaggttt tgataagagt cttagattta 158520agctcatcga cataatactt tactatgcac taaaactttt gaactttgtt attgttgtat 158580tgtccttttt tgttcttcat ttacttgaca ataggagact agattgtgtt acgacccaaa 158640ttccaagagc cattacagaa aacataatag acgttcacac tgatgctttg cgctaccaaa 158700tgtatgtgtt tttaaccatc aactccaaac gtattgttcc cttagtctta gcaacggcat 158760cttcacattc tttactagat ttacatgtgt cttaaatatc tttgtatatt ctaatttaat 158820cagacatagg ccgggcccac agagaagact tggggtctaa aagaaaagta tatgcattgg 158880aaagttgatg gagtgttttc accttttccc ttagtgctct ctgctttctc cttttctctg 158940caataataat gcagaactca taaaggaaga ttatgtgtat ttgaaatttt ttaatcacaa 159000acattttttt tttataaaag gaagatgaag atatgtgtga ttcaacaatg cggcgcttta 159060gaatgttctg atatcttgta tatgaatgat ggtttgctca tttggtaaga ttgtataaga 159120attgaagaaa tttgatggtt ttcacaactg atggattttt catgttttat gaggggaaaa 159180aaatacaact taaaaagtta aaattgcatg tccaatcaaa acttcaactt catatcaata 159240tgatttcata ccataatttc atatagccta tccaaatgag cccttaaaaa attgtaaact 159300tttatttgta aaatcgatct tccctttttc cccatgtatg acattaactc tctactgtag 159360ttaaattctt tttaaaattt gtgtaagtaa caaatataaa actatatata tttagatgaa 159420tatatttaga attcgatact ctaggatgtg aaagcagaat gtgatttatt ggacaaaggt 159480ctgaatattt tttactgcaa aatttgagtt tttttacgtt cctctcctat ttgggctata 159540ctgatgtgat tcgactaaaa tgcttgttag cctaaatgct tgtcaatcct tttttctgct 159600gatctgccct cttcggtccg ccctccatca ttattgtcac acatttgtta tagtgggtct 159660ttttttaata atatattatg gaataaagtt gatgtcgttt ttactaaagc aatcaatagg 159720aatgaagcac tatcattaat taataaagca ctggttgttc tttacctacc attatttaat 159780aaattaataa tgcaattatt cagaactcat acaatacaca ttccagtctg tttgagccat 159840tgccaactct aattattgtt tgatctcctc acccatctct ctgtactaga aaacaccttg 159900taccatatag gttttggatc tacgatcaat cagttaacaa tcgatcgatc gatctatcac 159960tgagctactg aggaacaaca ggaaattcaa taagttaaaa ttatattgag gtgagtgttt 160020tactcgaaaa aataaaaatg aaaatggact ttttttcgaa aaatcgatta aacttatgat 160080ttgaagagaa atcgattttc caaaagaaac agagtcgcca cttaattttt tagtaaaaat 160140caagaaaaaa acttaaggtt ttcaaaagat ttaatcagat aaaatcaatt gaaaataaaa 160200gggtttggag ttcaatgtac attccgagaa ggtgttgggc cctcgaaatg tccgctaact 160260tgcggttgac cggcgatttg actaaaaatg actttgacta aattttgaaa ttttaaccaa 160320gtaagaaaaa ctcatttatt ataacttgca gtatagctgt gcgattataa agcatagcta 160380tttatgaact gcttcattta ctataactct gtaaccatgt ttgcttggaa caaagaaaat 160440tagcgaattg gaagtggcaa atggtacttc caagacttct attcaattgg ataattgtta 160500cttgacgtgc taaaatgctg ttaaaccatc aatttattga atatttatag atttgtgtaa 160560acaaaaaatt agtctttcag taatcttgat tgtatgcttt cttttcttat gttccacttc 160620acaatatttt ctcagttggc acttattgca acatgcttga gctgcaaatt ttatcgcagc 160680aaacgagcta cactccaata ttaacaatat ttccttgttt gtttttgcag caaacgagct 160740acgctgacat gctggatcta gcagtgcagc acattcgaac ccttcaagat caggttcagg 160800tgggtgatta aattgatcaa ccttactgct ttgcagcttt acgtaatcta tcatgctgca 160860tgcacctagg tacaaaatta aaatacttat actctgttgc tcagactctt caatgatact 160920gttggatgtg ttagatttta tcctccaaaa gctatgcatt tttggaggat cagacactag 160980tacaacaatt ttggcccgag caacacatct tatattctca catgatgttt acaccaaacc 161040gaataatata accttaatgt ttttgggata gataatgtct aatatatttt gcaaaaaaaa 161100tgataaattt tctagcccca cctttatagc tgtgattgaa gtagtagaaa catatttttc 161160acattttctt tttcttatgc agaatctgaa tacagaactt gaaaactgca aatgtggatg 161220taagaaatca agtcaataac aaaatggaag gaagcacttt agctgaagat cgacttaaaa 161280ggatttttgt caccatgatt ttgcctctta gaaactcaaa tttgtacata aggaagtaaa 161340tttcatacta gttttttaaa gctaaaaggc gaactcttct tttttttttt ttttttttcc 161400tttccagtta gaagactacg tatatgattg ttttggatta tttgtagttt tttttttcca 161460tctaacattt acatttttaa tttagttttg agtttatttt ggttgaggat cataaagcta 161520attgatgatg ttgtacaaca actttctcta tttcattttt atgtaaaatg agtgggtaag 161580acattttctt gctttctgtt atgttcttct tgggaaacga ccatttacaa tttgcaaaga 161640agaaaagtaa ctctactcta caaggatttc atgtgtttta tttatcgaac tgatcatttt 161700cattgaaaaa aagtcattta attaatataa catttgtatt gagccataaa atcgatattt 161760cccttcttac caatgtatgt catgaatata tgtgattcat caatgtggcg ctttaggata 161820tttcgatatt ttgtttatga acgatgattt gctcattcgg taagattgca taggaaattg 161880actctttctt ttcgttgtta ttgctttcat tagtcgttgt ctcataacag actctctaac 161940taacattgga gaaataggaa actttcttca ctgaggctgt ctctttgcca tgacaagtta 162000gagttgcaaa ataaactcaa ctacaatttt tttttcttgt tgaatataag tacacctcac 162060aaatttacaa atgtatgtgt ttttgaccat caactccaaa cgtattgttc ccttggtctt 162120agcaacggca tctttgcttt ctttactaga tttacatgtg tcttaaaatt atttaaatat 162180ctttttatat tctaatttaa tcagacatcg gccaggccca cggagaagaa ttggggtcaa 162240aaagaaaagt gtatgtattg gaaagttgat gaagtgtttt caccttttcc cttagtgctc 162300tgtgctttct cctttttctc tacaataata atgcagaact cataaaggaa gattatgtgt 162360atttgaattt tttttaatca caaacatctt tattttataa aaggaacata aatatatgtt 162420tgattcatca atgcagcgct ttaggatgtt ccgatatctt gtatatgaat gatggtttgc 162480tcatttggtc agatttatac gaattgaaga aatttgatgg ttttcacaac tgatggattt 162540ttcctgtttt atgagggaaa acaaaataca atttaagaag tcaaaattgc atgtccaatc 162600aaaacgtcaa cttcatatca atatgatttc ataccataaa atcatatcgc ctatccaaac 162660gagcccttaa aaattgtaaa cttttatctg taaaatcgat tttcccttct tccccatgta 162720tggcatgaac tctctactgg tataagtaac aaatataaaa ctatatgtat ttagatgaat 162780atatttagaa ttcgatactc taggatgtga aagcagaatg tgctgattta ttggacaaag 162840gtctgaatat attttactgc aaaatttgag ggttttttcc atgttcctct cctatttggg 162900ctataccgat gtgtgattcg actaaaatgc ttgtcaatcc ttttttctga tctgcactct 162960tcggtccgcc ctccatcatt attaattgtc acacattcga tatgagtttc tttttgtttt 163020aaaaaaatga ctacttccgc gtgggtcttt ttttaataat atatcatgga ataattagac 163080attattctta aagacaaggg atagagttga tgttgtattt actaaagcaa tcaataggaa 163140tgaagcacta tcattaatta ataaagcact aattgttgtt tatctaccag taattaatag 163200attaataatg caataattca gaactcatac aatacacatt ccagtctgtt tgagccattg 163260ccaactctaa ttattgtttg atctcctctc tgtactagaa aacaccttgt gccatatagg 163320attctgatct acgaccaatc agttaacaat cgatcgatct atcactgagc tactgaggaa 163380caacaggaga ttcaataagt taagattgaa gtgagttcca ttgattcata atttattttc 163440atacttcatg gaattttgta tcctctgaat atatataccc cctcttccta atttaattaa 163500aagaaaacta tgaattagct gggtacgaaa attcgcatat aacttggttt cctgcgactt 163560tttctaccaa aaaattttcg atggaaatat cttcgtaggt aattattaca cgcgaatata 163620aaaatcccca agtaaattac ctggggattt gaatttcgca agtaaattac attcgaattt 163680tcttgcaaat aaatactaaa aatatccgag aaatttttag taaaaattcg tgaaaaaggg 163740aattttctta caattttttt ttttttgcaa aaattcctag gaaataattt gtgaataatc 163800attttaattc ttttagtttt taatttctgg atataccaat tatttttaat agttttgtct 163860ctgattatta tcttcttatg taattcttgt tattttattt tacaaccccc cacccctaca 163920cacatgttta cacccttaaa tgctcaacac aattaattgc acagccactc agaaaccaaa 163980gaaaccactg cccaaacatt catttagctt taactttcgt tctaccaaat ataaatgtta 164040aattgttaat gtactcattc gatgtggtac aaagatagca agggaattat tccaaatttt 164100tttagtacta ataaaaatat gacacaactt taaatatact ttacgtattt tatccacgtt 164160ctttaatcaa aaagagatta ttcttttaga atatatatat atatatatat atatatatat 164220atatatatat atatatatat atatatatat attttgtaat aataacatac gcagtaaaat 164280tttacttagc ggggtctgaa gaggatagtg tgtacgcaca ccttaacact agctcgtaga 164340gatagatata ttgttttcga aagacctcag ctcaagtgca tcaaacccaa ataaaagaag 164400aataaatcaa tgaaaacaaa taactattaa taagaaaagt agtgtaaagt ctataagaaa 164460gaacctctta aagaaattga cacaaattac ttaataagat gaatataatt taggtatttg 164520aaaaagtatc tatttttcaa aactttaaat aacatgatgt tattccaaat ttttataaac 164580attttggcct catatataat agaaatttct gtacaaataa aactataaaa tgataattta 164640aataatttat aagtttaaat aacatgatgt tattccaact cgaattattg tttgatctcc 164700tcacccatct ttatgtacca gacaacacca gctcgtacca catataagat ttggatctac 164760gatcaatcag ttaacaatag accgatctat cgctgagcta ctgaggaata acaaaagatt 164820tgataagtta agattgaggt acgttccatt cattcattat ttattttcat acttcatgaa 164880tctatatact ttctcttccc aatttaagtg tcttagtttg attagacaca aagtataaga 164940aataaagact tgattcttgt gatattaaat taaagatgta tgtagtcctt taaatcttat 165000gatcataaac ttgtcctgtg gaatgttaga attgaaaatt tactaaatat agaaagcgac 165060actcattcct gttggataca cttacacaag ttcatccagc tttttttgaa gttgaaattc 165120accaagtcat aaaaatgaag ctaacaataa aaaatgtcat tccaccaagt ctcctatagt 165180caagtaaacg aaagaaggaa tgaaaaagga gaaacaaatc atacaagaca ctagctagta 165240gcctagtaca gtagactcat actggtatat acatttttta gcttttgagg ttgagttaga 165300ctcatgtgtc atatttttat aaggtatcat aactagattc atactccttt ggactcatgg 165360tccatgctcc agttgggtct gtacatgaac gagggtgtca aagttcacat tgattgaaaa 165420acagaccgat aatttactta tatatatatg gacttaaata atcctcctct agtgagctat 165480tttagagatt gagtttggct caatgccata tctttagaat tatttaaata gtttttccat 165540attctaatct aattattact tgacaagtca gttggaattc ttcaattcca tgcacaatgg 165600tcaacacttt aatcaatgtc acaatcaaat ataaatgtgg ggaaggcgtg ggagcccatg 165660cgccttacaa gtccttctta catttctgac ccaataattt ttttgaggta aggcgtgggg 165720gtcctgcacg cgcctttgcc cctttggtct cttccatttt tttttgccct tttagaattt 165780tggcagaaaa taataattgt cctttaggct atggactcca attgtagaca actttttgtt 165840atgtagaatt tgctcaaaac atacaacttt ttgttttata gaatccggtc aaatacatac 165900aatttgcatt caatttattt gagttagaag cttggaactt ggaagtcact caattttggg 165960taattagcca tcatagtcgc tcaacttagc caagttagct cccatgattg ttttagctag 166020aaatacaatt tttggtacat atataatgac gtgatgacta taaattttta gaataaaata 166080tttttaaaaa ttaaaatcaa tatttaaatt tatttaggac ccaatccatc aaaactcaac 166140ctaattcttg ccacgaccaa tttaatgagt tgactatata aaaaatttct ttaaacaaac 166200tttatttctc ctttttccac ttgtgatttc tccatcacat gatatatctt ctctttctct 166260ttcaaatatg taaattttcc atttttttat tgttcttgtt ctatcagtca aatcattcat 166320tgcgaaagat taccaaaatg aatcgagttc tatatattac atggatagat aggttgagaa 166380aaaagtttat tgtttaaacg gttgttgcac tactgaaaaa ttaggtgaat aagatcattt 166440ttgtatttga gaagtcacta gttttttttt gttatttcat tcataaggtc attcaagttt 166500aaaagtaata catccggtca cttttaatga cataatattt gtaagtcatc ttcttcttga 166560attattttta tccctatata tagagagttg atttttcttt attgaagaaa attagaatca 166620agaagaaagg taattttctg

agataataac aattagttga gtgatcatat gaaaagagta 166680attatgcatt gtttgcaaga tgaaatggta cataagatgt ataatctttc acaagtatta 166740acatagcttc aaacactaga tattgaaaga catgaataaa aatgttgctc aaaattctac 166800ctcgtctcct tttcaacacc tccactcgcc ttagtttcac acctacaaac caatgcatct 166860gtaggtcgac acaagacatg tcacaccatc tccagcgcac gtctcttatt atattatcaa 166920tgctacttgc acttctagcg aatatatatg gattattttt aatttattta atattatatg 166980atcgcatatc catcttagca gttggtcaaa tttatgtggt ctaaccttgc aaaagtcacc 167040atcaggcaag atttcaatca actttgcccc tgctgagaag aattctcgtc cgagaggact 167100ataggggaca atccctatgc tatggtcaaa gaaatcaaaa actgctactc ttgaactcaa 167160atctaaaata aaaaatgtag aaaggaaaac acttcttatt atttagatac aaaaattaca 167220tactttcttt taacagatgc agtgtcccag ctagcttgca cgaacctcaa ccattccaag 167280gggtacctgc aatctctcac tggctacctg cataggtacc tgcaactcta tccaccaaaa 167340cttagatagc taggaagaca tcatctaatt tatgcattaa ctaccatttg aacctgaaac 167400ctcatggttc tcgaccccta tcatccacaa ctagccaaat acatatcact tgtcaaaccc 167460ctactcagct ttccaggagg acaatggtgg cgcgcgtctc tgaatttatc caacaaaata 167520agatttacca gtgtgaatta taagactcta aataagtaga actgatttat ttgcagtaag 167580aacgacaatg tgattaataa aggacaatgt gaaagttcac gtagccaatt aattagttct 167640ctgttgccaa ctgtcctctc cttccacaca ctttgattaa tcattgggaa tagatactaa 167700ccttccaata aagtagttgt ctctctgcat atatcataag ccatgttatt tcattaaata 167760atgcaaccat tgactttgaa agccaatagg aatgcatatt aacctgacaa tatattagct 167820atctaaaaat taacatgata attaaacaca ataattattc ataatacaac cattgacttt 167880tttgtaggat gtagatccag tcctttggtt aaccataatt attcatccac taatcttgtt 167940aagagtatga cacaaacaac atttcctcat tgttctaagt taattatgct catattaatc 168000ttttcttgtt agcttaatcc tactccaatc tttatcaggt atttttcaat aaatattttt 168060ctagatctcc ctttgaattt ttatcccttt ttaagtggaa tctgctttat gtataagtta 168120ctagttttta ggaacgtgtg ttgcacgtta tcccccaatt aatataaaat ttctacattt 168180taaaaaagtt ttaacttatg aaatagaaaa tattaacatc aaaatattgc agattttact 168240atttaaatct attcattaaa aacacaatta gtgggacgat ataatagcct ctggccctct 168300gatagtatgc aagagaattc ttgctctaat tttaataaat gcatgtaaaa atatttaaaa 168360aatttacaaa agaaaaatat gaagaaagat atctttttac aaaaccccaa ataggattgt 168420ttgtttagat ttaaaaatgc tatatcagat taagtgaaat ataaagttaa ttgcactacc 168480taataaaata tttctttttt ctctgccaag caagcatttt atgtaaatat gatatttgaa 168540tgttaaagaa aaagatacat tttcatttca caaaaaataa aatacctctc aattctataa 168600tccaaatgaa ttaaaaaata aatggttaat tatgaagtag tacaagtatt atgcatacaa 168660ctacatagat aatttaaatt caacaaattc tccttatatc attttgatgc ctcaacaacc 168720acaaagtcat aacttgaaaa gtattcacca gacaataaat ttttaactcc tatcttatgg 168780atcgtttttt taaaatctga agtacttcat ccatgcacaa agtattgcat aaatgtgtta 168840tatccagtac aacagtgcct gttgttatgt taaaataaaa atatgagcac agttgtgcct 168900aaataaagct gatacacaat aaagaaacaa aaattttgaa atagatgcat ggaaagaaat 168960acaagtttgc aatttgtcaa ttaaataagg attagacggt agaaacaacc acacaacaat 169020gtgcatagaa atagttagtt gcaatatgtg taccgtcaaa tgataatatg acgattaaag 169080ttaatttctt gttcgatcgt ggtattcaat aaaaagtcca aagttacttt acataaactt 169140gtcgcaagca gtaccatgtg aaatattttg ttacttacct ttgtttcttt taaccaaatg 169200tgcaatttga gcatcatggc ggagaagttg gttagcagta aacctcttgc aattatttgg 169260taattgataa atatcatgaa aaggaacaat agaggatata tatcactaat aacaattagc 169320aataactggg caaaacaaat tctcaccaaa aaataagagc caacgtttca tatgaaagga 169380gacgaaaagg aaaagttgat gaagagtttc ttggtaactg ctgtccttta acattattat 169440tattatgaga ttgtgaaata gactttaatt ttcattttct ccaaatttca gattatttga 169500actcagactt attactaaaa atatatacat ctataaatat aaatatggtg attaagatta 169560tttaagtaat gagtacattg atattgctgg ccaaaaggat atttccttaa aatatttata 169620tttaatcaaa tttaaattta gtagaaggac aaaatggtaa ttcaactttt cactttggag 169680cttcccacta ataataataa taataataat aatatatata tatatatata tatatatata 169740tatatatata tatatatata tatatatata tatatgatca acaactcttt ttctaggatt 169800ggatcagaac ttggagatac aaaaaggaaa aagaagatcg atactcaact actcgtcaca 169860ctcgaaaatt gtaagatatg agtatatatt aacttgctat tgctttcaaa aatctcaaag 169920ttcttaaatt gatacaacaa ctgagttatt aattcatttt accaataaac ttcatggttc 169980caaaactaca aatattgtga taataaaaaa tggatatcac acataataat tagtttttgc 170040tctgttgaac cagttaaaga agtgttttat ttttgttttc ctctttaatt ttctatttta 170100ttttattttt taaaaaagtg atcaacagca gatggctggt tatatgtctt tatagataca 170160aattgggggt aggggaattg aaccgtcata aggtgaaagt tgtgatattc aaccgactga 170220gcgactaatc gtgctatcct tctgcacaca ctttttttaa ttaatcattg ggaatgcatt 170280agttgtttct ttgtactaat aattaataga gaaaaagctc aaatatgtca tccaactttg 170340agaagaaaaa aaccctcatt taatagttgg gcatgtcatt gccattacat aaatgaccca 170400ttatgccatt gatttttaac agtcaggact tatgttttgc aacactattt ttttccatct 170460ggcttcttat tagaggtcca tgtcaccaaa attagactag tcgaaaagta agttcaaata 170520tgccactgaa ctgtaacaag atgttcatct atgtcattca ttaacagttg gattcaacag 170580cagatggctg gttatatgtt tttatagata caaatcgggg gtagggattt gaactatcat 170640aaggtgaaaa ttctgatatc caaccaactg agcgactaat cgtactatcc ttccccccac 170700acattttttt tattattaat cattgggtat gcattagttg tttctctgta ctagtaatta 170760atagggaaaa aattagctaa atgatgtcat ccaactttga ggggaaaaaa acctcattta 170820tgtcatccat taatagttgg gttcaatcac atgcctttgt cataacagaa atgacccatt 170880atgccattat tttttaatat tcagattttg tggttttgca acaccatttt tccatatggc 170940ctcttattag aggtccacgt caccaaaatg aaactggtca aaaaaaagtt caaatatgcc 171000attgaactgt gacaaaatgt tcatatatgg cattcgttaa tagttggatt caaccatgtc 171060actgcagttt aatttggaat tataatccaa cccattaaca ccatacccga tccaccataa 171120cccatttccc ttttatttct cctacaaact cttagatcca aaatttctcc cattttaatg 171180atgaatttat atgtcagttt cttgtcaatt gatcaattta agtgtttggg gagaaataaa 171240aagggaaata ggttagggtg ggtcgagtgt gatgttatgg gtcgggtttt aatttcaaat 171300ttaatttaat taggttggtt tatattggca cgtggacctc taataagaga ccacgtgaaa 171360aaaaattaat tttgcgagat catgaaacct gaccattaaa aagtagtatg aatgggtccc 171420ttctgtaacg acaatggaat ggttgagccc aataattaac agatggcata gataaacctt 171480ttttcaaaat ttgatgcata tttgaacttt ttttatcagt ttaattttgg taatgtggac 171540ctctaataag agaccacgtg gaaagaacga ttttgctaac catcaaacca gctacctgtt 171600aaaaaataat ggcacgaatg gaccatttct gtaaagcagc ggcatgattg agcccaacta 171660ttaacgggag gcacatcaac aagcattttc ccgatgtttg atggcaaatt ttaactcttt 171720tacattattg ttaactcaaa cacaatttta aagaaatttt atgtgatttt ttaatagttc 171780atatctaggc acgtgcaacg atactactat atattataat acctaagtgt tagttcaagc 171840aagtattgtg aacataaaag ctacgacaat tgtaaggaaa atgattccct tttataagtg 171900aggaaaatca tttcccccct tttagatgaa acttgtttta atttctcgac ttctatcata 171960gtttagacac acattagatg agcttgagat ctaaaaaata agcaatttta agttgatgtt 172020tcattttcca tttctatatg ttaagttagt cggaagaata attataatga ccatcttgtc 172080agggccaagc tttcgtgata tgcatcgtcg agctcaaaag tgtagtgaac ttgtgttatg 172140tattgtaact ttttttttta ctttcgtctc ttatttgggc aattcctgtg tgtgattcaa 172200ctaagatgtt tgtcaatcct tttctttgac ctactcttat cggtcctccc tccttcatta 172260atgtcacaca tttgatatta gtttcttttt gtttaaagaa aatgactatt agttgagtga 172320aatgtgtctg agatgatatg cactatttga attagatgaa atggtatata aactgtatta 172380tctttcataa gaattaacgt agctaaaaac actagatatt gaaatacatg aataaaaatg 172440ttgttaatga acctaattcc aagagttggt gaacttgcag gaaattttca agagaagatg 172500gctgaaaatg aaattgagga aatgttagat cacctaagaa ggatcaagag tgaacgtgat 172560ctgtctagcg ccaggattga aaaaattaag aaacttgaaa tagcgctaag agttttgaga 172620acctttataa agtgtcatca tgttcttttt cgtgattcct tagtcaaaca caaaaagaat 172680gccaaattga ctatggcaat gcttcaccag gtattggatg ggattccaga tgaatgtaaa 172740gctaacctta atctggaaag gctagaatca catttgttgg aattcatgga acgtgatacc 172800attttaaata ataattatga gttgaatgat cttgatcttg atcttgatct gtcagaatgt 172860atggattgcc tcgaaaagaa tctaaatgat actgatactc tgtctggaat gtgttaggtc 172920tgacccttct gaagaaaacc atgaaatact cagattttta aaggaactga aagttgttca 172980aaagaaactg agatttttgt catatttata tgccacagag ataaatggtt acgtcaacca 173040tgagaagctg ggatgtttgg agactcgaat tcagttcatg gctaacaatg tgggacattt 173100ttgtcttgct ttttctgata ttataaatgg tattgatgag gatgaggcta atgaaatctt 173160taatacacct ccttatctat tattcttgat tgtgttagtg gagctggaaa tgaagaagat 173220ttttcagagt gaactaaagg attcaaagtt tactcattca agaactttca aggacaagaa 173280attaccaaaa ggattttctc atcatctcca caaactattg atgtatctca gaaaagaaaa 173340gctcgagaac tttcttgatg atgtctctgc tcaaaatatt gatgtggcaa tagagttctt 173400gttggtcttc cttgatgctg atgtgtcaaa tcatgttatt aatggtaact ggttgaatga 173460ggttatggaa aaagttggag ctatagcagg tgatgttcta tatgtcatgg aaaaacttct 173520tcctagctct ataaacagag atgacactag caaaataaat ctttgctcgt tacaaatatt 173580ggagaaaact aaagatctga aggcacaagt ggaaacttac tacaaatcct tgaaatttac 173640tccatctcag ttctccacct tttgtggatt gagctatctg gattctcttt tatggaaatt 173700gaatgagatg tcgaaatcta aatctgattt agatttcttg atgaaacctc tttttggtaa 173760tttggagaaa gagctatcaa gtcatatatc cattttagag aaggagctct catctttatc 173820atccattttc agagatgttc taaaggtgca ccatgaacat aaaattccta aaaatctcca 173880aagacgtacc atcaatttgg catatgaagc tgagtttgcc attgactcta ttcagtataa 173940tgcttttttg catatttttt gctcacttcc tacaatctta aaagagatca agcacattaa 174000tgcacaggtg actgagatgt ggtcagcaga cgttgctctt aagccttgct atgtggtagc 174060actatttaaa cacctgccaa ctcaacataa caatccagtg attgatgagg agatagtggg 174120ttttgggaaa gacacagaaa aaatgattca gtgtttgatt agaggtacaa atgagctaga 174180cgtcgtccca attgtaggca tggggggaca agggaaaacg acaattgcta gaaaggtgta 174240caatagtgac aacattgttt ctcattttga tgttcgagca tggtgcatcg tttcccaaac 174300atataaccgg agaacgctat tacaagagat ttttagtcaa gttaccggtt ccaaggacaa 174360gggggataag gatgacatcc ttgctgacga gttgaggaaa atcttaatgg gcaagagata 174420tctcattgta ttggatgata tgtgggattg tatggcatgg gatgacttga ggctttgttt 174480tccagatgtt ggaaatagaa gcagaatcgt agtaacaact cgacttgaga aagtgggtga 174540gaaagtcaag tactacactg atccccgggt acc 1745733957PRTPhytophthora infestans 39Met Arg Ser Ile Gln Leu Leu Ile Ile Ala Leu Val Ala Phe Leu Ala 1 5 10 15 Cys Cys Ser Ala Thr Pro Ala Pro Pro Gln Val Ser Leu Ser Phe Leu 20 25 30 Pro Val Gln Arg Arg Ser Leu Arg Thr Asp Thr Thr Leu Asp Ser Glu 35 40 45 Asp Asn Asn Glu Asp Ser Gly Glu Arg 50 55 40187PRTPhytophthora infestans 40Ser Val Trp Lys His Val Lys Val Arg Trp Trp Leu Glu Thr Glu Lys 1 5 10 15 Ser Asp Asp Phe Val Arg Lys Ala Leu Lys Leu Asn Gly Leu Asp Asp 20 25 30 Thr Ala Met Lys Ala His Lys Asn Tyr Lys Tyr Tyr Ala Tyr Phe Ala 35 40 45 Lys Lys Ala Glu Asp Tyr Leu Phe Asn Lys Trp Leu Arg Asn His Val 50 55 60 Pro Thr Phe Glu Ala Trp Lys Ser Leu Asn Leu Gly Lys Ile Thr Lys 65 70 75 80 Ala Asp Gln Leu Lys Glu Ile Ala Asn Thr Lys Asn Phe Ile Ser Tyr 85 90 95 Ser Arg Phe Val Lys Gln Tyr Asp Asp Asn Val Val Ser Thr Leu Asn 100 105 110 Ala Gly Tyr Asn Pro Pro Val Val Ala Val Ala Arg Gly Ala Ser Glu 115 120 125 Ala Glu Ile Thr Ala Arg Thr Met Ile Met Ala Ser Ala Arg Arg Asp 130 135 140 Asp Asp Val Ala Lys Val Leu Leu Gly Leu Thr Lys Pro Gly Tyr Pro 145 150 155 160 Arg Arg Val Leu Asp Gly Asn Ala Leu Thr Gln His Asp Glu Tyr Lys 165 170 175 Tyr Tyr Gln Leu Phe Lys Glu Ala Lys Thr Ser 180 185

Claims

1. Isolated nucleotide sequence encoding a resistance protein that is able to confer resistance to Phytophthora infestans when expressed in a plant, wherein said nucleotide sequence encodes a protein that is encoded by the nucleotide sequence of FIG. 10 or the protein depicted in FIG. 11 or a nucleotide sequence that codes for a protein that has an identity of at least 95% with said protein produced by the nucleotide sequence of FIG. 10 or the protein depicted in FIG. 11.

2. Nucleotide sequence according to claim 1, wherein said sequence is the part of one of the sequences of FIG. 10, which is coding for a protein.

3. A method for providing at least partial resistance or increasing resistance in a plant against an oomycete infection comprising providing a plant or a part thereof with a nucleotide sequence according to claim 1 or a functional fragment thereof.

4. Method according to claim 3, wherein said oomycete comprises Phytophthora, preferably Phytophthora infestans.

5. Method according to claim 3, wherein said plant or part thereof is also provided with a nucleotide sequence encoding a further resistance gene selected from Table 1.

6. A method for obtaining a potato plant that is resistant against an oomycete infection by crossing a potato plant that has the R8 gene from Solanum demissum with a potato plant of interest and testing the offspring of said cross for the presence of a nucleotide sequence according to claim 1.

7. A method according to claim 6, wherein said potato plant that has the R8 gene is a potato plant from the variety `Sarpo Mira`.

8. A method for breeding an oomycete resistant tetraploid plant, preferably a Phytophthora resistant tetraploid plant, comprising a. increasing or decreasing the ploidy level of the gametes of a diploid plant that already contains a nucleic acid sequence as defined in claim 1; b. using said gametes in a cross with gametes of a tetraploid plant; and c. selecting the offspring of said cross for the presence of said nucleic acid sequence.

9. A method according to claim 6, wherein said oomycete resistant plant further comprises one or more further resistance genes selected from Table 1.

10. A method for selecting a plant or plant material or progeny thereof for its susceptibility or resistance to an oomycete infection, said method comprising the steps of testing at least part of said plant or plant material or progeny thereof for the presence or absence of a nucleic acid as defined in claim 1.

11. A method according to claim 6, wherein said testing involves detecting the presence of at least part of the nucleotide sequence as shown in FIG. 12 or FIG. 14 and is performed with a primer or a probe that specifically binds to said nucleotide sequence.

12. A transgenic or tetraploid cell comprising a nucleic acid according to claim 1.

13. A vector comprising a nucleic acid sequence according to claim 1.

14. A vector according to claim 13, further comprising the promoter and/or terminator with which the gene is naturally associated.

15. A transgenic or tetraploid host cell comprising a nucleic acid according to claim 1 or a vector according to claim 13.

16. A transgenic or tetraploid plant cell comprising a nucleic acid according to claim 1 or a vector according to claim 13.

17. A transgenic or tetraploid plant comprising a cell according to claim 16.

18. A plant produced by a method according to claim 6.

19. A part derived from a plant according to claim 17.

20. A protein encoded by an isolated or recombinant nucleic acid according to claim 1 or a functional fragment thereof.

21. An antibody that binds to the protein of claim 20.