VACCINE

Abstract

Compositions and methods for the treatment or prevention of Gram-negative bacterial strain infection are provided herein. Methods for the manufacture of said compositions are also provided herein.

Description

[0001] This application is filed pursuant to 35 U.S.C. .sctn.120 as a continuation of U.S. Ser. No. 13/583,419, a United States National Phase Application of International Patent Application Serial No. PCT/EP/2011/053665 filed Mar. 11, 2011, which claims priority from Provisional Application No. 61/312,959 filed Mar. 11, 2010.

TECHNICAL FIELD

[0002] The present invention relates to the field of Gram-negative bacterial immunogenic compositions and vaccines, their manufacture and the use of such compositions in medicine. More particularly, it relates to immunogenic compositions comprising outer membrane proteins of Gram-negative bacteria which are involved in uptake of extracellular zinc. Such proteins are promising antigens as they show high conservation between strains belonging to many different Gram-negative bacterial species.

BACKGROUND

[0003] Gram negative bacteria are the causative agents for a number of human pathologies and there is a need for effective vaccines to be developed against many of these bacteria. There is accordingly a need to identify further antigens, such as outer membrane proteins, which are well conserved within, and possibly between, Gram-negative species in order that such antigens may be useful as vaccine components.

[0004] In the case of serogroup B of N. meningitidis, the development of a vaccine has been impeded due to the fact that the polysaccharide capsule is poorly immunogenic owing to its immunologic similarity to human polysialylated glycoproteins such as neural cell adhesion molecule. Strategies for vaccine production have therefore concentrated on the surface exposed structures of the meningococcal outer membrane such as PorA, but have been hampered by the high antigenic variability of the major outer membrane proteins among strains.

[0005] However, N. meningitidis has recently been found to express an outer membrane protein, "TdfI" or "ZnuD" (BASB082 of WO 00/55327; locus tag NMB0964 in the sequenced genome of N. meningitidis serogroup B; herein SEQ ID NO. 1) involved in the uptake of extracellular zinc. This is surprising as it was previously believed that zinc crosses the outer membrane by passive diffusion through porins, and this finding is the first discovery of a protein involved in zinc uptake across the outer membrane of a Gram-negative bacterium.

SUMMARY OF THE INVENTION

[0006] The present inventors have identified homologues of TdfI in many strains from numerous Gram-negative bacterial species. The conservation of the amino acid sequence between these species may reflect the importance of TdfI in the active uptake of zinc in environments having low levels of free zinc. TdfI and its identified homologues, as well as homologues yet to be identified, represent a promising source of antigens for the development of vaccines against Gram-negative infection. Due to the amino acid sequence conservation of secondary structure amongst TdfI and its identified homologues, such vaccines may confer protection not only to individual serotypes, sub-species and strains, but across all members of a bacterial species, or other taxonomic level. Indeed, as shown in the Examples, TdfI can induce cross-bactericidal antibodies against almost all Neisserial strains tested regardless of serogroup, supporting the potential of this outer membrane protein to be used as a universal N. meningitidis antigen. A further surprising discovery on which the present invention is predicated is the identification of another N. meningitidis outer membrane protein involved in uptake of extracellular zinc, "TdfH" (BASB024 of WO 00/11182; herein SEQ ID NO. 50). Whilst TdfI is believed to be involved in the acquisition of free zinc from the extracellular environment, it appears that TdfH binds to non-free, complexed zinc, such as zinc bound to calprotectin as shown in the Examples. Homologues of TdfH have been identified in several strains from different Gram-negative bacterial species, and a high degree of amino acid sequence conservation is observed. Hence, analogous to TdfI, TdfH and homologues thereof are expected to be useful as a source of antigens for vaccination against Gram-negative infection and disease.

[0007] Following the work underpinning this present invention, it is now believed that N. meningitidis, and many other Gram-negative species, have active mechanisms for uptake of zinc across the outer membrane. Such mechanisms, which involve at least TdfI and TdfH, may be necessary due to the low levels of free zinc in the host (for example in the human body), especially in certain compartments such as the respiratory tract. As shown in the Examples, the viability of cells lacking TdfI or H is impaired in conditions of low free zinc, or where the zinc is complexed to e.g. calprotectin, respectively. Vaccination with the aim of targeting the host immune system to Gram-negative bacteria expressing TdfI or TdfH promises to provide effective prevention or treatment against infection by such bacteria, and such vaccines may have significant cross-protectivity within, and possibly between, Gram-negative species as a result of the conserved nature of the proteins. The protection conferred by a vaccine comprising an antigen based on one of these proteins could result from a bactericidal immune response towards the bacterium in question, or simply from a prevention of the zinc uptake activity the protein. It is envisaged that a vaccine comprising both TdfI- and TdfH-derived antigens, thereby targeting both identified zinc uptake mechanisms, may be particularly effective.

[0008] The present invention accordingly provides immunogenic compositions or vaccines, and methods, for eliciting an immune response against Gram-negative bacteria expressing outer membrane proteins involved in uptake of extracellular zinc, such as TdfI and/or TdfH and homologues thereof. Methods for preparing such compositions or vaccines are also provided.

BRIEF DESCRIPTION OF DRAWINGS

[0009] FIGS. 1A, 1B, 1C AND 1D. Detection of TdfI on Western blot. (FIG. 1A) HB-1 grown in TSB (lane 1), RPMI (lane 2) and the tdfI knockout strain grown in RPMI (lane 3). (FIG. 1B) HB-1 grown in RPMI with increasing amounts of TSB added. (FIG. 1C) HB-1 grown in RPMI (lane 1), supplemented with 0.5 .mu.M zinc (lane 2) or 1 .mu.M zinc (lane 4). (FIG. 1D) HB-1 grown in RPMI (lane 1), with increasing concentrations of TPEN (0.1, 0.5 and 1 .mu.M in lanes 2-4, respectively)

[0010] FIG. 2. TdfI expression in wild type and zur mutant strains. (FIG. 2) The presence of TdfI in cell lysates of HB-1 and the zur mutant grown in RPMI, RPMI with 600 nM zinc or TSB was assessed by Western blot analysis.

[0011] FIGS. 3A and 3B. Topology model of TdfI. (FIG. 3A & FIG. 3B) The plug domain is colored dark grey, the beta strands light gray and the extracellular loops white. The histidine/aspartic acid stretches are boxed.

[0012] FIGS. 4A and 4B. Zinc binding and transport by TdfI. (FIG. 4A) Zinc binding to outer membrane vesicles either containing or not TdfI was measured by a PAR competition assay (FIG. 4B) Intracellular zinc concentrations as measured by ICP-MS of the wild-type strain, the tdfI mutant and the tong mutant. (FIG. 4C) RPMI+Zinc in wild type and znuA knockout.

[0013] FIG. 5. Zinc regulation of TdfI is highly conserved in meningococci. (FIG. 5) Western blot of cell lysates of the indicated strains grown in RPMI with or without added zinc. .sup.a Clonal group designations taken from (36); --indicates that the strain was typed by Multi-Locus Enzyme Electrophoresis but could not be assigned to a specific clone.

[0014] FIG. 6. Protein profile of the TdfI vaccine. (FIG. 6) Outer membrane vesicles used to immunize mice for antiserum production were separated by SDS-PAGE and stained with Coomassie brilliant blue.

[0015] FIG. 7. Impact of IPTG on expression of TdfI on cells used in SBA. (FIG. 7) See Example 1.

[0016] FIG. 8. (FIG. 8 & FIG. 8(Cont.)) Amino acid sequence alignment of TdfI of N. meningitidis strains MC58 with those of 053422, FAM18 and Z2491, the carrier strains .alpha.14, .alpha.153 and .alpha.275 The TonB box (Tb), the plug domain, the loops and the transmembrane domains (Tm) are marked above the sequence and the His- and Asp-rich stretches are underlined.

[0017] FIG. 9. (FIG. 9-FIG. 9(Cont.ii)) Amino acid sequence alignment of the TdfI homologues. The histidine aspartic acid rich stretches are highlighted in grey.

[0018] FIG. 10. ZnuD peptide array: Individual response of human sera obtained for convalescent patients or healthy carriers. (FIG. 10-FIG. 10(Cont.i)) See Example 3. This FIG. corresponds to FIG. 8 in U.S. 61/312,959. The samples listed top-to-bottom in the legends to FIG. 10 correspond to the samples listed from top-left to bottom-right (reading by rows) in the legends to FIG. 8 in U.S. 61/312,959.

[0019] FIG. 11. Expression level of ZnuD in function of culture media. (FIG. 11) FACs analysis using anti-ZnuD monoclonal antibody of H44/76 WT strain grown on either MH agar (gray shaded profile) or MH+20 .mu.M TPEN agar (thick line) and on Azur H44/76 strain cultivated on MH agar (thin line).

TABLE-US-00001 TABLE 2 Level of anti-fHbp and anti-ZnuD antibodies produced in mice and guinea-pigs after immunization with control OMVs and ZnuD OMVs Mice GP Tdfl fHbp Tdfl fHbp Ctrl OMVs .sup. 73 .sup.a 1342 10 335 ZnuD OMVs 15096 59314 5893 2733 .sup.a Geometric mean titers (expressed in EU/ml) from three pools of sera per group.

TABLE-US-00002 TABLE 3 Serum bactericidal titers (GMT for 50% killing) performed in presence of baby rabbit complement on a panel of 14 serogroup B strains cultivated with or without TPEN. H44/ NZ98/ M01- M01- M01- M01- DE M05- M05- M98- Strains 76 254 240101 240355 240013 240149 MC58 18025 10690-06 0240471 17540 0240072* 76067 250771 fHbp B B B A A B B B B B B B A A family fHbp ++ + + + + + ++ +/- +/- +/- - - - +/- expression Tdfl + + + + + + + + + + + + + + expression Animal Culture species Treatment condition Serum bactericidal titers Mouse fHbp B MH agar 3383 110 494 50 50 2293 3993 3804 50 50 50 50 50 50 MH-TPEN 3539 388 509 50 50 2104 5786 4373 169 50 50 50 50 50 agar Mouse Control MH agar 103 50 50 50 50 71 88 50 50 50 50 50 50 50 OMVs MH-TPEN 265 112 50 50 50 50 304 182 50 50 50 50 50 50 agar ZnuD MH agar 3605 69 519 50 50 3116 1734 50 50 50 50 50 50 50 OMVs MH-TPEN 7903 1854 1733 3310 852 3374 17486 4872 937 197 286 50 50 143 agar GP Control MH agar 55 50 50 50 50 50 67 67 50 50 50 50 50 50 OMVs MH-TPEN 115 50 50 50 50 50 50 50 50 50 50 50 50 50 agar ZnuD MH agar 331 50 50 50 50 94 240 234 50 50 50 94 122 50 OMVs MH-TPEN 1751 2592 2153 2497 3706 2961 1846 752 1781 2005 667 50 50 2021 agar .sup.a Geometric mean titers (expressed in EU/ml) from three pools of sera per group. GMT above the threshold for a positive result (titer .gtoreq. 128) are shown in bold

TABLE-US-00003 TABLE 4 Impact of culture conditions (ZnuD expression or not) on the bactericidal activity of sera on H44/76 WT strain and and delta fHbp or delta znuD H44/76 strains H44/76 strains SBA delta delta Species condition Treatment WT fHbp Tdfl Mouse MH agar CTRL OMVs .sup. 97 .sup.a 50 50 ZnuD OMVs 5332 50 3925 MH-TPEN CTRL OMVs 304 50 216 agar ZnuD OMVs 13397 1581 9603 Guinea- MH agar CTRL OMVs 58 50 138 pig ZnuD OMVs 310 50 409 MH-TPEN CTRL OMVs 118 50 129 agar ZnuD OMVs 2402 3678 765 .sup.a Geometric mean titers (expressed in EU/ml) from three pools of sera per group. See Example 3.

[0020] FIGS. 12A and 12B: OMVs used for immunization. SDS-PAGE (FIG. 12A). Presence of fHbp in OMVs preparation (ELISA) (FIG. 12B).

TABLE-US-00004 TABLE 5 Tdfl homologues No. % amino identity acids over No. identical length of amino to SEQ ID SEQ ID SEQ ID Accession No. Bacteria name acids No. 1 No. 1 No. AAF62323 Neisseria meningitidis MC58 758 758 100 1 YP_986285 Acidovorax sp. JS42 695 252 33 2 YP_002967458 Acinetobacter baumannii 803 325 43 3 YP_003208315 Acinetobacter baumannii 803 328 43 4 YP_001708692 Acinetobacter baumannii 803 325 43 5 ZP_04661004 Acinetobacter baumannii AB900 685 306 40 6 YP_001847801 Acinetobacter baumannii ACICU 685 306 40 7 YP_001083098 Acinetobacter baumannii ATCC 17978 803 327 43 8 YP_001706181 Acinetobacter baumannii SDF 685 306 40 9 ZP_06058868 Acinetobacter calcoaceticus RUH2202 691 309 41 10 ZP_06064377 Acinetobacter johnsonii SH046 680 302 40 11 ZP_06064957 Acinetobacter junii SH205 685 304 40 12 ZP_06068135 Acinetobacter lwoffii SH145 684 288 38 13 ZP_05362119 Acinetobacter radioresistens SK82 699 309 41 14 YP_045356 Acinetobacter sp. ADP1 693 296 39 15 ZP_03824903 Acinetobacter sp. ATCC 27244 696 303 40 16 ZP_05823027 Acinetobacter sp. RUH2624 670 277 37 17 ZP_03612144 Actinobacillus minor 202 823 339 45 18 ZP_04754640 Actinobacillus minor NM305 823 340 45 19 YP_001053620 Actinobacillus pleuropneumoniae L20 782 317 42 20 ZP_00133885 Actinobacillus pleuropneumoniae serovar 1 782 317 42 21 str. 4074 YP_001651932 Actinobacillus pleuropneumoniae serovar 3 790 317 42 22 str. JL03 YP_693858 Alcanivorax borkumensis SK2 689 265 35 23 YP_931882 Azoarcus sp. BH72 691 271 36 24 YP_002798586 Azotobacter vinelandii DJ 682 256 34 25 NP_886739 Bordetella bronchiseptica RB50 713 264 35 26 NP_882547 Bordetella parapertussis 711 264 35 27 NP_881648 Bordetella pertussis Tohama I 713 263 35 28 YP_001633406 Bordetella petrii 684 274 36 29 YP_003278330 Comamonas testosteroni CNB-2 711 248 33 30 ZP_03543249 Comamonas testosteroni KF-1 711 250 33 31 YP_001564964 Delftia acidovorans SPH-1 730 257 34 32 YP_002553299 Diaphorobacter sp. TPSY 693 251 33 33 ZP_02478325 Haemophilus parasuis 29755 809 327 43 34 YP_002474986 Haemophilus parasuis SH0165 797 321 42 35 YP_718275 Haemophilus somnus 129PT 868 294 39 36 YP_001785276 Haemophilus somnus 2336 868 304 40 37 AAK29743 Mannheimia haemolytica 766 346 46 38 ZP_04977416 Mannheimia haemolytica PHL213 790 310 41 39 ZP_05988256 Mannheimia haemolytica serotype A2 str. 654 311 41 40 BOVINE ZP_05990414 Mannheimia haemolytica serotype A2 str. 793 313 41 41 BOVINE AAU94646 Moraxella catarrhalis 818 343 45 42 ZP_05920958 Pasteurella dagmatis ATCC 43325 796 340 45 43 NP_246018 Pasteurella multocida subsp. multocida str. 809 339 45 44 Pm70 NP_246367 Pasteurella multocida subsp. multocida str. 805 301 40 45 Pm70 NP_245682 Pasteurella multocida subsp. multocida str. 925 270 36 46 Pm70 ZP_03841874 Proteus mirabilis ATCC 29906 697 224 30 47 YP_001171782 Pseudomonas stutzeri A1501 672 251 33 48 ZP_01302207 Sphingomonas sp. SKA58 686 255 34 49

TABLE-US-00005 TABLE 6 TdfH homologues No. % amino identity acids over No. identical length of amino to SEQ ID SEQ ID SEQ ID Accession No. bacteria name acids No. 1 No. 1 No. AAF41853.1 Neisseria meningitidis MC58 921 921 100 50 YP_003254871 Aggregatibacter actinomycetemcomitans 530 296 32 51 YP_003254872 Aggregatibacter actinomycetemcomitans D11S-1 365 245 27 52 YP_003008699 Aggregatibacter aphrophilus NJ8700 924 520 56 53 ZP_00367782 Campylobacter coli RM2228 758 286 31 54 ZP_00371680 Campylobacter upsaliensis RM3195 758 290 31 55 ZP_01788784 Haemophilus influenzae 3655 921 478 52 56 ZP_04464966 Haemophilus influenzae 6P18H1 950 487 53 57 ZP_04466359 Haemophilus influenzae 7P49H1 915 499 54 58 YP_248875 Haemophilus influenzae 86-028NP 915 486 53 59 ZP_05849940 Haemophilus influenzae NT127 910 486 53 60 ZP_01790772 Haemophilus influenzae PittAA 921 482 52 61 ZP_01792691 Haemophilus influenzae PittHH 915 489 53 62 ZP_01794868 Haemophilus influenzae PittII 915 491 53 63 ZP_00154314 Haemophilus influenzae R2846 934 488 53 64 ZP_00157057 Haemophilus influenzae R2866 929 476 52 65 NP_439373 Haemophilus influenzae Rd KW20 913 486 53 66 ZP_05848904 Haemophilus influenzae RdAW 913 486 53 67 YP_718388 Haemophilus somnus 129PT 915 350 38 68 YP_001783402 Haemophilus somnus 2336 917 356 39 69 AAT76666 Moraxella catarrhalis 902 467 51 70 ZP_05921174 Pasteurella dagmatis ATCC 43325 860 326 35 71

[0021] See Example 3.

[0022] FIG. 13: multiple alignment of TdfI homologues. (FIG. 13-FIG. 13(Cont.x)).

[0023] FIG. 14: multiple alignment of TdfH homologues. (FIG. 14-FIG. 14(Cont.v)).

[0024] FIG. 15: calprotectin binding to TdfH (FIG. 15).

[0025] FIG. 16: calprotectin binding to TdfH in presence of Zn or Mn. (FIG. 16).

DETAILED DESCRIPTION OF THE INVENTION

[0026] The present invention is based on the discovery that N. meningitidis expresses outer membrane proteins involved in zinc uptake, at least one of which (TdfI) is found in most tested N. meningitidis strains as well as in numerous other Gram-negative bacterial species. TdfI from N. meningitidis serogroup B has been found to induce cross-bactericidal antibodies to N. meningitidis strains of all serogroups, and hence these proteins and their homologues in other Gram-negative species represent promising antigens for protecting against Gram-negative infection.

[0027] As used herein, the terms "TdfI protein" and "TdfH protein" are used to refer to the respective proteins as identified in N. meningitidis as well as to homologues thereof.

[0028] Hence, in one aspect the invention provides an immunogenic composition or vaccine comprising an antigen which is capable of raising an immune response, if necessary when coupled to a carrier protein, which recognises an outer membrane protein of at least a first gram-negative bacterial strain, wherein said protein is involved in uptake of extracellular zinc.

[0029] As used herein, by "immunogenic composition" or "vaccine" is meant a composition comprising at least one antigen which is capable of generating an immune response when administered to a host. "Immune response" as used herein means a B-cell antibody response.

[0030] An "antigen", according to the context of the present invention, is any substance which stimulates an immune response in the body. In one embodiment, the antigen is a polypeptide derived from the outer membrane protein of the first Gram-negative strain that is recognised by the immune response raised by said antigen. By "derived" in this sense is meant that the antigen is generated using said outer membrane protein as a starting point physically (e.g. by target mutation, truncation etc.) or intellectually (e.g. using the known sequence of said protein to design a synthesised polypeptide). As used herein, "polypeptide" means any chain of two or more amino acid residues linked by peptide bonds. The immunogenic composition or vaccine may comprise, in addition to said antigen, one or more additional antigens.

[0031] By "carrier protein" is meant any protein to which the antigen is coupled or attached or conjugated, typically for the purpose of enhancing or facilitating detection of the antigen by the immune system. The term is intended to cover both small peptides and large polypeptides (>10 kDa). The carrier may be any peptide or protein. It may comprise one or more T-helper epitopes. The carrier protein may be, for example, tetanus toxoid (TT), tetanus toxoid fragment C, non-toxic mutants of tetanus toxin, diphtheria toxoid (DT), CRM197, Pneumolysin (Ply), protein D, PhtD or PhtDE.

[0032] The immune response raised by the antigen may recognise an outer membrane protein of a first (i.e. of a single) bacterial strain, or it may recognise an outer membrane protein present on two or more strains, in which case said proteins need not be identical between the strains but must share similar epitopes such that each of the respective proteins is recognised by the immune response raised by the antigen. Of particular interest is the raising of an immune response which recognises multiple, or all, strains of a given Gram-negative species, wherein said strains differ by at least one of serogroup, serotype, serosubtype or the precise amino acid sequence of the outer membrane protein from which the antigen is derived. Cross-protection may also extend beyond individual species.

[0033] The term "outer membrane protein" as used herein means a polypeptide or protein integral to or attached to or expressed on the outer membrane of Gram-negative bacteria. The protein may be an integral membrane protein, i.e. "embedded" within the membrane, optionally having portions exposed periplasmically and/or extracellularly. Alternatively, the protein may be attached to the extracellular surface of the outer membrane, either directly to the lipid bilayer or to an integral protein. Suitably, the outer membrane protein is between 600-1000 amino acids in length.

[0034] Involvement of the protein in zinc uptake means that the protein binds free or complexed extracellular zinc, and optionally transports the bound zinc across the outer membrane. Whether or not a Gram-negative outer membrane protein is involved in zinc binding, and optionally transport, would be readily ascertainable to one skilled in the art, and the Examples provide several ways in which this may be accomplished, including modelling of protein structure, a zinc sequestration competition assay and an assay of the regulation of expression of the protein in response to external zinc concentration. More particularly, the involvement of said outer membrane protein in zinc uptake is such that zinc binding is reduced for outer membrane vesicles lacking said protein and accumulation of zinc is reduced in cells lacking said protein, and/or is such that cells lacking said protein have a reduced ability to grow in the presence of calprotectin as sole zinc source.

[0035] It is to be noted that the outer membrane protein recognised by said immune response may, in certain embodiments, not consist of the amino acid sequence of an NMB0964 polypeptide according to PCT/EP2009/052689 or a fragment thereof as disclosed therein, SEQ ID NO. 2 of WO 00/55327 or a fragment thereof as disclosed therein, SEQ ID NO. 606 of WO 99/57280 or a fragment thereof as disclosed therein, or SEQ ID Nos. 2, 4 and 6 of WO 00/11182 or a fragment thereof as disclosed therein. Additionally or alternatively, said first Gram-negative bacterial strain which expresses said outer membrane protein is not a Neisserial strain.

[0036] In one embodiment, the expression of said outer membrane protein is upregulated in response to low zinc availability. Said upregulation of expression relative to the level of expression of the protein during growth on a complex, zinc-containing medium such as tryptic soy broth (TSB) may be 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10-fold or greater. Such upregulation of expression may be achieved using a zinc chelator, suitably TPEN (N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine) at for example 1-25 .mu.m, or a chemically-defined medium low in zinc such as Catlin medium.

[0037] In one embodiment, said first Gram-negative bacterial strain which expresses the outer membrane protein recognised by said immune response is a strain which infects body compartments of humans and/or animals having low levels of free zinc. Such compartments include, for example, the respiratory tract and the blood as well as the urinary tract and intestine. Suitably, said first Gram-negative bacterial strain belongs to a species which infects the respiratory tract such as Brucella sp., Coxiella sp., Pseudomonas sp., Acinetobacter sp., Moraxella sp., Chlamydia psittaci, Chlamydia trachomatis, Haemophilus influenzae, Haemophilus parasuis, Haemophilus somnus, Legionella pneumophila, Actinobacillus pleuropneumoniae, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchoseptica, Mannheimia haemolytica, Pasteurella dagmatis and Pasteurella multocida. In one embodiment, said low level of free zinc is a result of binding of zinc to molecules such as calprotectin. By a low level of free zinc is meant under 5, 4, 3, 2, 1, 0.9, 0.8, 0.7, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0.05 or 0.01 .mu.M free zinc, such as is present in Roswell Park Memorial Institute medium 1640 (RPMI) which has around 1.69 .mu.M zinc by inductively coupled plasma mass spectroscopy. As used herein, "zinc" refers to Zn.sup.2+.

[0038] In a particular embodiment, said first Gram-negative strain belongs to a species or genus selected from the group consisting of Bordetella; Bordetella pertussis; Borrelia; Borrelia burgdorferi; Brucella; Brucella melitensis; Brucella ovis; Chlamydia; Chlamydia psittaci; Chlamydia trachomatis; Escherichia; Escherichia coli; Haemophilus; Haemophilus influenzae; Legionella; Legionella pneumophila; Neisseria; Neisseria gonorrhoeae; Neisseria meningitidis; Pseudomonas; Pseudomonas aeruginosa; Yersinia; Yersinia enterocolitica; Moraxella; Moraxella catarrhalis; Shigella; Shigella flexneri; Shigella dysenteriae; Shigella boydii; Coxiella; and Coxiella burnetii.

[0039] In another embodiment, said first Gram-negative strain belongs to a species or genus selected from the group consisting of Acidovorax; Acinetobacter; Acinetobacter baumannii; Acinetobacter baumannii; Acinetobacter calcoaceticus; Acinetobacter johnsonii; Acinetobacter junii; Acinetobacter lwoffii; Acinetobacter radioresistens; Actinobacillus; Actinobacillus minor; Actinobacillus pleuropneumoniae; Aggregatibacter; Aggregatibacter actinomycetemcomitans; Aggregatibacter aphrophilus; Alcanivorax; Alcanivorax borkumensis; Azoarcus; Azotobacter; Azotobacter vinelandii; Bordetella; Bordetella bronchiseptica; Bordetella parapertussis; Bordetella pertussis; Bordetella petrii; Campylobacter; Campylobacter coil; Campylobacter upsaliensis; Comamonas; Comamonas testosteroni; Delftia; Delftia acidovorans; Diaphorobacter; Haemophilus; Haemophilus influenzae; Haemophilus parasuis; Haemophilus somnus; Mannheimia; Mannheimia haemolytica; Moraxella; Moraxella catarrhalis; Neisseria; Neisseria gonorrhoeae; Neisseria meningitidis; Pasteurella; Pasteurella dagmatis; Pasteurella multocida; Proteus; Proteus mirabilis; Pseudomonas; Pseudomonas stutzeri; and Sphingomonas.

[0040] More particularly, the outer membrane protein of said first Gram-negative strain comprises an amino acid sequence selected from the group consisting of SEQ ID No. 1; SEQ ID No. 2; SEQ ID No. 3; SEQ ID No. 4; SEQ ID No. 5; SEQ ID No. 6; SEQ ID No. 7; SEQ ID No. 8; SEQ ID No. 9; SEQ ID No. 10; SEQ ID No. 11; SEQ ID No. 12; SEQ ID No. 13; SEQ ID No. 14; SEQ ID No. 15; SEQ ID No. 16; SEQ ID No. 17; SEQ ID No. 18; SEQ ID No. 19; SEQ ID No. 20; SEQ ID No. 21; SEQ ID No. 22; SEQ ID No. 23; SEQ ID No. 24; SEQ ID No. 25; SEQ ID No. 26; SEQ ID No. 27; SEQ ID No. 28; SEQ ID No. 29; SEQ ID No. 30; SEQ ID No. 31; SEQ ID No. 32; SEQ ID No. 33; SEQ ID No. 34; SEQ ID No. 35; SEQ ID No. 36; SEQ ID No. 37; SEQ ID No. 38; SEQ ID No. 39; SEQ ID No. 40; SEQ ID No. 41; SEQ ID No. 42; SEQ ID No. 43; SEQ ID No. 44; SEQ ID No. 45; SEQ ID No. 46; SEQ ID No. 47; SEQ ID No. 48; SEQ ID No. 49; SEQ ID No. 50; SEQ ID No. 51; SEQ ID No. 52; SEQ ID No. 53; SEQ ID No. 54; SEQ ID No. 55; SEQ ID No. 56; SEQ ID No. 57; SEQ ID No. 58; SEQ ID No. 59; SEQ ID No. 60; SEQ ID No. 61; SEQ ID No. 62; SEQ ID No. 63; SEQ ID No. 64; SEQ ID No. 65; SEQ ID No. 66; SEQ ID No. 67; SEQ ID No. 68; SEQ ID No. 69; SEQ ID No. 70; and SEQ ID No. 71, wherein SEQ ID No. 1 is TdfI from N. meningitidis strain MC58 and SEQ ID Nos. 2 to 49 are homologues thereof and SEQ ID No. 50 is TdfH from N. meningitidis strain MC58 and SEQ ID Nos. 51 to 71 are homologues thereof.

[0041] In another aspect, the present invention provides an immunogenic composition or vaccine comprising an antigen, which antigen comprises a polypeptide having at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 99% or at least 100% identity to an amino acid sequence selected from the group consisting of SEQ ID No. 1; SEQ ID No. 2; SEQ ID No. 3; SEQ ID No. 4; SEQ ID No. 5; SEQ ID No. 6; SEQ ID No. 7; SEQ ID No. 8; SEQ ID No. 9; SEQ ID No. 10; SEQ ID No. 11; SEQ ID No. 12; SEQ ID No. 13; SEQ ID No. 14; SEQ ID No. 15; SEQ ID No. 16; SEQ ID No. 17; SEQ ID No. 18; SEQ ID No. 19; SEQ ID No. 20; SEQ ID No. 21; SEQ ID No. 22; SEQ ID No. 23; SEQ ID No. 24; SEQ ID No. 25; SEQ ID No. 26; SEQ ID No. 27; SEQ ID No. 28; SEQ ID No. 29; SEQ ID No. 30; SEQ ID No. 31; SEQ ID No. 32; SEQ ID No. 33; SEQ ID No. 34; SEQ ID No. 35; SEQ ID No. 36; SEQ ID No. 37; SEQ ID No. 38; SEQ ID No. 39; SEQ ID No. 40; SEQ ID No. 41; SEQ ID No. 42; SEQ ID No. 43; SEQ ID No. 44; SEQ ID No. 45; SEQ ID No. 46; SEQ ID No. 47; SEQ ID No. 48; SEQ ID No. 49; SEQ ID No. 50; SEQ ID No. 51; SEQ ID No. 52; SEQ ID No. 53; SEQ ID No. 54; SEQ ID No. 55; SEQ ID No. 56; SEQ ID No. 57; SEQ ID No. 58; SEQ ID No. 59; SEQ ID No. 60; SEQ ID No. 61; SEQ ID No. 62; SEQ ID No. 63; SEQ ID No. 64; SEQ ID No. 65; SEQ ID No. 66; SEQ ID No. 67; SEQ ID No. 68; SEQ ID No. 69; SEQ ID No. 70; and SEQ ID No. 71, optionally wherein said antigen is capable of raising an immune response, if necessary when coupled to a carrier protein, which recognises the amino acid sequence.

[0042] In this aspect, the polypeptide of which the antigen is (at least in part) comprised is capable of raising an immune response which recognises at least the amino acid sequence with which said polypeptide shares a degree of amino acid sequence identity. The degree of identity may be 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100%. In one embodiment, the polypeptide has the same or substantially the same immunogenic activity as the amino acid sequence with which said polypeptide shares such amino acid sequence identity.

[0043] "Identity," as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as the case may be, as determined by comparing the sequences. In the art, "identity" also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. "Identity" can be readily calculated by known methods, including but not limited to those described in (Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heine, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991; and Carillo, H., and Lipman, D., SIAM J Applied Math., 48: 1073 (1988). Methods to determine identity are designed to give the largest match between the sequences tested. Moreover, methods to determine identity are codified in publicly available computer programs. Computer program methods to determine identity between two sequences include, but are not limited to, the GAP program in the GCG program package (Devereux, J., et al., Nucleic Acids Research 12(1): 387 (1984)), BLASTP, BLASTN (Altschul, S. F. et al., J Mol. Biol. 215: 403-410 (1990), and FASTA (Pearson and Lipman Proc. Nati. Acad. Sci. USA 85; 2444-2448 (1988). The BLAST family of programs is publicly available from NCBI and other sources (BLAST Manual, Altschul, S., et al., NCBI NLM NIH Bethesda, Md. 20894; Altschul, S., et al., J Mol. Biol. 215: 403-410 (1990). The well known Smith Waterman algorithm may also be used to determine identity.

[0044] Parameters for polypeptide sequence comparison can include the following:

[0045] Algorithm: Needleman and Wunsch, J. Mol Biol. 48: 443-453 (1970)

[0046] Comparison matrix: BLOSSUM62 from Henikoff and Henikoff,

[0047] Proc. Natl. Acad. Sci. USA. 89:10915-10919 (1992)

[0048] Gap Penalty: 8

[0049] Gap Length Penalty: 2

[0050] A program useful with these parameters is publicly available as the "gap" program from Genetics Computer Group, Madison Wis. The aforementioned parameters are the default parameters for peptide comparisons (along with no penalty for end gaps).

[0051] Parameters for pairwise alignment using the ClustalW program can include:

[0052] Gap Open Penalty: 10.00

[0053] Gap Extension Penalty: 0.10

[0054] Protein weight matrix: Gonnet series

[0055] DNA weight matrix: IUB

[0056] Parameters for multiple alignment using the ClustalW program can include:

[0057] Gap Opening Penalty: 10.00

[0058] Gap Extension Penalty: 0.20

[0059] Delay divergent sequences: 30%

[0060] DNA Transitions Weight: 0.50

[0061] Protein weight matrix: Gonnet series

[0062] DNA weight matrix: IUB

[0063] Use negative matrix: OFF

[0064] Unless otherwise stated, herein "identity" is expressed over the entire length of the reference, rather than the test, sequence. Multiple alignments of SEQ ID Nos. 1 and 50 with their respective identified homologues are shown Figures FIGS. 10 and 11, and the identity values are shown in Tables 5 and 6.

[0065] In certain embodiments of this aspect, said antigen or polypeptide may optionally not have the amino acid sequence of an NMB0964 polypeptide according to PCT/EP2009/052689 or a fragment thereof as disclosed therein, SEQ ID NO. 2 of WO 00/55327 or a fragment thereof as disclosed therein, SEQ ID NO. 606 of WO 99/57280 or a fragment thereof as disclosed therein, or SEQ ID Nos. 2, 4 and 6 of WO 00/11182 or a fragment thereof as disclosed therein.

[0066] In a further aspect, the present invention provides an immunogenic composition or vaccine comprising an antigen, which antigen comprises a polypeptide comprising an immunogenic fragment of a sequence selected from the group consisting of SEQ ID No. 1; SEQ ID No. 2; SEQ ID No. 3; SEQ ID No. 4; SEQ ID No. 5; SEQ ID No. 6; SEQ ID No. 7; SEQ ID No. 8; SEQ ID No. 9; SEQ ID No. 10; SEQ ID No. 11; SEQ ID No. 12; SEQ ID No. 13; SEQ ID No. 14; SEQ ID No. 15; SEQ ID No. 16; SEQ ID No. 17; SEQ ID No. 18; SEQ ID No. 19; SEQ ID No. 20; SEQ ID No. 21; SEQ ID No. 22; SEQ ID No. 23; SEQ ID No. 24; SEQ ID No. 25; SEQ ID No. 26; SEQ ID No. 27; SEQ ID No. 28; SEQ ID No. 29; SEQ ID No. 30; SEQ ID No. 31; SEQ ID No. 32; SEQ ID No. 33; SEQ ID No. 34; SEQ ID No. 35; SEQ ID No. 36; SEQ ID No. 37; SEQ ID No. 38; SEQ ID No. 39; SEQ ID No. 40; SEQ ID No. 41; SEQ ID No. 42; SEQ ID No. 43; SEQ ID No. 44; SEQ ID No. 45; SEQ ID No. 46; SEQ ID No. 47; SEQ ID No. 48; SEQ ID No. 49; SEQ ID No. 50; SEQ ID No. 51; SEQ ID No. 52; SEQ ID No. 53; SEQ ID No. 54; SEQ ID No. 55; SEQ ID No. 56; SEQ ID No. 57; SEQ ID No. 58; SEQ ID No. 59; SEQ ID No. 60; SEQ ID No. 61; SEQ ID No. 62; SEQ ID No. 63; SEQ ID No. 64; SEQ ID No. 65; SEQ ID No. 66; SEQ ID No. 67; SEQ ID No. 68; SEQ ID No. 69; SEQ ID No. 70; and SEQ ID No. 71, wherein said immunogenic fragment consists of an amino acid sequence having 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 30, 40, 50 or more contiguous amino acids of said selected sequence, and wherein said immunogenic fragment is capable of raising an immune response, if necessary when coupled to a carrier protein, which recognises said selected sequence.

[0067] An "immunogenic fragment" as used herein is a polypeptide having an amino acid sequence that is entirely the same over its length as part, but not all, of an amino acid sequence of the invention (i.e. of one of SEQ ID Nos. 1 to 71), and which is capable of raising an immune response which recognises at least the amino acid sequence of which it is a fragment. In one embodiment, the immunogenic fragment has the same or substantially the same immunogenic activity as the amino acid sequence of which said fragment is a fragment. Suitably said immunogenic fragment is, at least in part, from an extracellular portion of the amino acid sequence of which it is a fragment. Said fragments may otherwise be, for example, truncated derivatives of the amino acids sequences of the invention, such as a continuous series of residues that includes an amino- or carboxyl-terminal amino acid sequence. Degradation forms of the amino acids sequences of the invention may also be used.

[0068] In certain embodiments of this aspect, said antigen or polypeptide or immunogenic fragment may optionally not have the amino acid sequence of an NMB0964 polypeptide according to PCT/EP2009/052689 or a fragment thereof as disclosed therein, SEQ ID NO. 2 of WO 00/55327 or a fragment thereof as disclosed therein, SEQ ID NO. 606 of WO 99/57280 or a fragment thereof as disclosed therein, or SEQ ID Nos. 2, 4 and 6 of WO 00/11182 or a fragment thereof as disclosed therein.

[0069] Said antigen or said polypeptide or said immunogenic fragment may in particular be derived from a TdfI protein, in which case the amino acid sequences with which said polypeptide has amino acid sequence identity, or of which said immunogenic fragment is a fragment, are selected from the group consisting of SEQ ID No. 1; SEQ ID No. 2; SEQ ID No. 3; SEQ ID No. 4; SEQ ID No. 5; SEQ ID No. 6; SEQ ID No. 7; SEQ ID No. 8; SEQ ID No. 9; SEQ ID No. 10; SEQ ID No. 11; SEQ ID No. 12; SEQ ID No. 13; SEQ ID No. 14; SEQ ID No. 15; SEQ ID No. 16; SEQ ID No. 17; SEQ ID No. 18; SEQ ID No. 19; SEQ ID No. 20; SEQ ID No. 21; SEQ ID No. 22; SEQ ID No. 23; SEQ ID No. 24; SEQ ID No. 25; SEQ ID No. 26; SEQ ID No. 27; SEQ ID No. 28; SEQ ID No. 29; SEQ ID No. 30; SEQ ID No. 31; SEQ ID No. 32; SEQ ID No. 33; SEQ ID No. 34; SEQ ID No. 35; SEQ ID No. 36; SEQ ID No. 37; SEQ ID No. 38; SEQ ID No. 39; SEQ ID No. 40; SEQ ID No. 41; SEQ ID No. 42; SEQ ID No. 43; SEQ ID No. 44; SEQ ID No. 45; SEQ ID No. 46; SEQ ID No. 47; SEQ ID No. 48; and SEQ ID No. 49.

[0070] In a further embodiment, said polypeptide or immunogenic fragment of the immunogenic composition or vaccine of the invention comprises at least one of the following amino acid motifs:

[0071] 91-G-S/A/V-S/A/V/G-X-P-V/I/M-V/I-R-G-Q/M/L-X-G/S/A-X-R;

[0072] 116-D-V/A/M-S/A-2(X)-S/G-P/A-D-H-T/A/N-V/I;

[0073] 154-G-L/V/A/I-V/I-N/D-V/I/L-X-D-X-K/R-I/L/V-P;

[0074] 250-G-X-G/S/A-Y/F/W/V-G/S/T/N-X-Q/R/L-3(X)-Y-G-L/I/V-L/I/P-G/A-H/D;

[0075] 334-R-X-D/E-X-R/K/Q/D-G/T/S/A-E/Q/S/D-3(X)-P-3(X)-I/F/V/L-3(X)-R/A/- K/Q-5(X)-D/N/R/G-Y-X-H-X-E;

[0076] 510-R-X-P-X-A/P/V/T-Q/E/M-E-L/M-Y/F-A/S/Y/T-X-G-X-H-X-A-T/L/S-X-T/S- /A-F/Y/I/V-E/Q-(1-9)X-G-D/N/Q-X-X-L;

[0077] 595-Y-X-Q/G-2(X)-A-X-F/L/Y/I-X-G-X-E/D-G/A/V-3(X)-Y/F/H/Q-8(X)-G/S/- A/T-X-F/S-G-D-X-V/I-R/K/N-A/G; and

[0078] 657-P/A-R-X-P/S/A-A/P/G-X-R-L/V/A-S/G.

[0079] The number preceding each motif indicates, in a still further embodiment, the position in the sequence of SEQ ID No. 1 of the first amino acid of the sequence of SEQ ID No. 1 that corresponds to that motif when SEQ ID No. 1 and said polypeptide comprising said motif are aligned. In one embodiment, said polypeptide or immunogenic fragment comprises 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or more further amino acids at the N- and/or C-terminus of said motif from the sequence contiguous to said motif beginning at the position of said first amino acid of SEQ ID No. 1 or at the equivalent positions of SEQ ID No. 2 to 49.

[0080] The motifs are depicted using the standard single-letter amino acid code, expressed in N- to C-terminal direction from left to right. For example, "-G-" at a particular position relative to the other amino acids of the motif indicates a requirement for a glycine residue at that position, whilst for example "-S/A/V-" specifies that a serine or an alanine or a valine residue (in no order of preference) must occupy that position. An unspecified position, i.e. which may be any amino acid residue, is represented by "X", and multiple contiguous such residues are indicted by, for example, 3(X) meaning "-X-X-X-".

[0081] As mentioned above, said antigen or said polypeptide or said immunogenic fragment may in particular be derived from a TdfI protein. Alternatively, said antigen or said polypeptide or said immunogenic fragment may be derived from a TdfH protein, in which case the amino acid sequences with which said polypeptide has amino acid sequence identity, or of which said immunogenic fragment is a fragment, are selected from the group consisting of SEQ ID No. 50; SEQ ID No. 51; SEQ ID No. 52; SEQ ID No. 53; SEQ ID No. 54; SEQ ID No. 55; SEQ ID No. 56; SEQ ID No. 57; SEQ ID No. 58; SEQ ID No. 59; SEQ ID No. 60; SEQ ID No. 61; SEQ ID No. 62; SEQ ID No. 63; SEQ ID No. 64; SEQ ID No. 65; SEQ ID No. 66; SEQ ID No. 67; SEQ ID No. 68; SEQ ID No. 69; SEQ ID No. 70; and SEQ ID No. 71.

[0082] In a further embodiment, said polypeptide or immunogenic fragment of the immunogenic composition or vaccine of the invention comprises at least one of the following amino acid motifs:

[0083] 81-R-SIT-V/I-P-G-A-Fly-T-Q/N-Q/V/L/I-D-K/Q-G/A/S-S/Q-G-X-VA-S-V/L-N- -V/I-R-G-X-S/N/T-G-F/L-G-R-V/A-N-S/T-M/Q-V/I-D-G-V/I-S/T-Q-T-F;

[0084] 156-F-S/N/D/E-G-S/T/A/K-A/G/S/N-G-I/L/V/A-N-S/T/A-L-X-G-S-A-N-L/F-R- /K-T-L/I-G/N/S-V/A-D/N-D;

[0085] 226-S-X-R/K/Q-X-VP/SA-S/A-Q-N/D-Y/F-R/K-V/I-G-G-G;

[0086] 333-L-F/A/L/V-K-L/I/F/V-E/R-Y-X-G/N/D/S-V/D/K/H-4(X)-T/G/N/I-A/L-Q/- N/S-F/L/I/Y-R-X-L/M/Y-X-T/N-X-I/L/V-G/A/S-S/T/G-R-K/R/N/S-IA-X-N-R/D/K/N-N- /T-Y-Q;

[0087] 491-P-X-G-S/K/E-Q-X-F/I-N/H/K/I-T/S-FP/VA-Y-F/L/I-D;

[0088] 574-N-H/Y-S-V/A/L/M-S/T/I/M-INF-S-A-X-F/Y/L/I-G/D/S/H-D/T/P-Y/G/L-F- -M/N/S/T-P-F-X-S/T/G-Y/F-S/A-R/H/K-T/S-H-R-M/I/V/A-P-N-I/V-Q/K/R-E-M/Y/V-Y- /F-F-S/T; and

[0089] 846-E/D-V/I-K/Q-N-L/A/V-F/L-D-R/K-R/L/K/N-Y-I/V/M-D/N-P/A-L-D/Y.

[0090] The number preceding each motif indicates, in a still further embodiment, the position in the sequence of SEQ ID No. 50 of the first amino acid of the sequence of SEQ ID No. 50 that corresponds to that motif when SEQ ID No. 50 and said polypeptide comprising said motif are aligned. In one embodiment, said polypeptide or immunogenic fragment comprises 1, 2, 3, 4, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100 or more further amino acids at the N- and/or C-terminus of said motif from the sequence contiguous to said motif beginning at the position of said first amino acid of SEQ ID No. 50 or at the equivalent positions of SEQ ID No. 51 to 71. The motifs are depicted as described above in connection with TdfI motifs.

[0091] As mentioned above, the immunogenic composition or vaccine of the invention may comprise one or more antigens additional to that defined supra. In an embodiment in which said immunogenic composition or vaccine comprises an antigen which is capable of raising an immune response which recognises a TdfI protein, said at least one additional antigen may be one which is capable of raising an immune response which recognises a TdfH protein as defined herein. Conversely, in an embodiment in which said immunogenic composition or vaccine comprises an antigen which is capable of raising an immune response which recognises a TdfH protein, said at least one additional antigen may be one which is capable of raising an immune response which recognises a TdfI protein as defined herein. Alternatively or additionally, such additional antigens may include any antigen, which antigen may or may not raise an immune response to the same Gram-negative strain or species as the "first" antigen, or even to a Gram-negative bacterium or taxonomic group thereof. Suitably, said at least one additional antigen is a fHbp polypeptide or a Hsf polypeptide or a Hap polypeptide or a NadA polypeptide or a lipo28 polypeptide, for instance from N. meningitidis.

[0092] fHbp can mean the GNA1870 protein from Neisseria, Hsf the NMB0992 (WO99/31132; SEQ ID NO: 2 and 4 [BASB029] in WO99/58683) protein from Neisseria, Hap the NMB1985 (WO99/55873-BASB006) protein from Neisseria, NadA the NMB1994 protein from Neisseria, lipo28 the GNA2132 or NMB2132 protein from Neisseria. GNA and NMB numbers referred to herein refer to polypeptides encoded by Neisseria meningitidis group A and B, respectively, genome sequences available from www.neisseria.org.

[0093] Additionally, where a protein is specifically mentioned herein, it is suitably a reference to a native, full-length protein, and to its natural variants, but it may also encompass antigenic fragments thereof. These are fragments (often specifically described herein) containing or comprising at least 15 amino acids, suitably at least 20 amino acids, at least 30 amino acids, at least 40 amino acids or at least 50 amino acids, taken contiguously from the amino acid sequence of the protein. Antigenic fragments may also be immunogenic fragments. It is further envisaged that reference to proteins and protein sequences herein includes a polypeptide comprising an immunogenic fragment of 7, 10, 12, 15, 20, 30, 40 or 50 (or more) contiguous amino acids from said protein sequence or from the amino acid sequence of said protein (optionally wherein said immunogenic fragment is capable of eliciting--if necessary when coupled to a protein carrier--an immune response which can recognise said protein or said protein sequence).

[0094] Hsf: Hsf has a structure that is common to autotransporter proteins. For example, Hsf from N. meningitidis strain H44/76 consists of a signal sequence made up of amino acids 1-51, a head region at the amino terminus of the mature protein (amino acids 52-479) that is surface exposed and contains variable regions (amino acids 52-106, 121-124, 191-210 and 230-234), a neck region (amino acids 480-509), a hydrophobic alpha-helix region (amino acids 518-529) and an anchoring domain in which four transmembrane strands span the outer membrane (amino acids 539-591).

[0095] Although full length Hsf may be used in immunogenic compositions of the disclosure, various Hsf truncates and deletions may also be used depending on the type of immunogenic composition or vaccine.

[0096] Where Hsf is used in a subunit composition or vaccine, a portion of the soluble passenger domain may be used; for instance the complete domain of amino acids 52 to 479, most suitably a conserved portion thereof, for instance the sequence of amino acids 134 to 479. Suitable forms of Hsf may be truncated so as to delete variable regions of the protein disclosed in WO01/55182.

[0097] Suitable variants would include the deletion of one, two, three, four, or five variable regions as defined in WO01/55182. The above sequences and those described below, can be extended or truncated by up to 1, 3, 5, 7, 10 or 15 amino acids at either or both N or C termini.

[0098] Suitable fragments of Hsf therefore include the entire head region of Hsf, suitably containing amino acids 52-473. Additional suitable fragments of Hsf include surface exposed regions of the head including one or more of the following amino acid sequences; 52-62, 76-93, 116-134, 147-157, 157-175, 199-211, 230-252, 252-270, 284-306, 328-338, 362-391, 408-418, 430-440 and 469-479.

[0099] Where Hsf is present in an outer membrane vesicle preparation, it may be expressed as the full-length protein or as a variant made up of a fusion of amino acids 1-51 and 134-591 (yielding a mature outer membrane protein of amino acid sequence 134 to the C-terminus). Suitable forms of Hsf may be truncated so as to delete variable regions of the protein disclosed in WO01/55182. Suitable variants would include the deletion of one, two, three, four, or five variable regions as defined in WO01/55182. In one aspect the first and second variable regions are deleted.

[0100] Suitable variants would delete residues from between amino acid sequence 52 through to 237 or 54 through to 237, more suitably deleting residues between amino acid 52 through to 133 or 55 through to 133. The mature protein would lack the signal peptide.

[0101] Hap: Computer analysis of the Hap-like protein from Neisseria meningitidis reveals at least three structural domains. Considering the Hap-like sequence from strain H44/76 as a reference, Domain 1, comprising amino-acid 1 to 42, encodes a sec-dependant signal peptide characteristic of the auto-transporter family, Domain 2, comprising amino-acids 43 to 950, encode the passenger domain likely to be surface exposed and accessible to the immune system, Domain 3, comprising residues 951 to the C-terminus (1457), is predicted to encode beta-strands likely to assemble into a barrel-like structure and to be anchored into the outer-membrane. Since domains 2 and 3 are likely to be surface-exposed, well conserved (more than 80% in all strains tested) and could be produced as subunit antigens in E. coli, it represents an interesting vaccine candidate (see also Pizza et al. (2000), Science 287: 1816-1820).

[0102] Immunogenic compositions of the disclosure may comprise the full-length Hap protein, suitably incorporated into an OMV preparation. Immunogenic compositions of the disclosure may also comprise the passenger domain of Hap which in strain H44/76 is composed of amino acid residues 43-950. This fragment of Hap would be particularly advantageously used in a subunit composition of the disclosure. The above sequence for the passenger domain of Hap can be extended or truncated by up to 1, 3, 5, 7, 10, 15, 20, 25, or 30 amino acids at either or both N or C termini.

[0103] The immunogenic composition or vaccine of the invention, in one embodiment, comprises a bacterial outer membrane vesicle preparation, which preparation comprises the antigen of the composition or vaccine. Such a composition or vaccine may be produced by a method provided as another aspect of the invention, which method comprises culturing at least a first gram-negative bacterial strain which produces said antigen, wherein said antigen is produced at a level sufficient to provide for production of outer membrane vesicles that, when administered to a subject, raise a protective response against infection by at least said first gram-negative bacterial strain; preparing outer membrane vesicles from the cultured strain; and combining said outer membrane vesicles with a pharmaceutically acceptable carrier or excipient to produce an immunogenic composition suitable for administration to a subject.

[0104] By "a level sufficient to provide for production of outer membrane vesicles that, when administered to a subject, raise a protective response against infection by at least said first gram-negative bacterial strain" is meant that sufficient antigen is produced such that upon administration of outer membrane vesicles comprising said antigen an immune response is induced that prevents, retards the development of, or reduces the severity of a disease that is caused by said first Gram-negative strain, or diminishes or completely eliminates the symptoms of the disease.

[0105] As is shown in the Examples, the expression of TdfI is regulated in response to the free zinc concentration, such that decreasing levels of free zinc correlate with an upregulation of TdfI expression. (The expression of TdfH is believed also to be influenced by free zinc levels, although to a lesser extent.) Hence, in said method, the culturing of said bacterial strain may be in a medium comprising a zinc chelator, suitably at a concentration of 0.01-100, 0.1-10, 0.3-5, or 0.5-1 .mu.M, in order to upregulate expression of said antigen. Said zinc chelator may, for example, be TPEN. As an alternative to the use of a zinc chelator, said culturing according to the method may take place in Catlin medium.

[0106] In one embodiment of the method, the antigen is expressed in said first gram-negative bacterial strain from an expression vector comprising a polynucleotide encoding said antigen.

[0107] Additionally or alternatively, said antigen is produced by expression from a heterologous and/or strong promoter, i.e. in one embodiment such a promoter is engineered into the chromosome of said bacterial strain such that it is operably linked with the polynucleotide sequence encoding said antigen. As is well known to one skilled in the art, the term "heterologous" refers to two biological components that are not found together in nature. The components may be host cells, genes, or regulatory regions, such as promoters. Although the heterologous components are not found together in nature, they can function together, as when a promoter heterologous to a gene is operably linked to the gene. A "strong" promoter is a promoter which drives expression of a polynucleotide sequence operably linked thereto at a high level. What constitutes a high level, in the context of a bacterial strain belonging to a particular species, would be well known to one skilled in the art, as would be the identity of strong promoters in such a strain.

[0108] Further as regards said method, the step of preparing the outer membrane vesicles from the cultured strain may be performed using detergent extraction, or may be achieved by alternative means. In the former case, said extraction may involve the use of 0-0.5%, 0.02-0.4%, 0.04-0.3%, 0.06-0.2%, 0.08-0.15% or 0.1% detergent. Preferably, the detergent is deoxycholate.

[0109] As mentioned above, the expression of said antigen may be upregulated in the outer membrane vesicles produced from said first gram-negative bacterial strain, for example as a result of culturing in conditions of low free zinc. Hence, the expression of said antigen may be upregulated in outer membrane vesicle preparations in immunogenic compositions or vaccines of the invention which comprise such preparations. Such upregulation may be relative to the expression of said antigen in an outer membrane vesicle preparation from a wild-type bacterium of the same strain. In particular, expression of said antigen may be upregulated 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10-fold or greater.

[0110] Alternatively or additionally to culturing in conditions of low free zinc, in order to achieve such upregulation of expression of said antigen a host cell from which said outer membrane vesicle preparation is derived may be genetically modified in production of said antigen. Such modification may involve disruption of functional expression of an endogenous protein, which protein in a non-genetically-modified host cell represses expression of said antigen. Hence, following such targeted disruption of the endogenous repressor protein the expression of said antigen is no longer repressed, i.e. is upregulated relatively. In one embodiment, the endogenous repressor protein is the Neisserial or Gram-negative bacterial Zur repressor. Alternatively or additionally to disruption of repressor protein expression, the host cell may be modified by the introduction of a heterologous and/or strong promoter operably linked to the polynucleotide encoding the antigen. Such promoters are discussed supra. Said introduced promoter may be inducible, i.e. the promoter may be "switched on" or upregulated in response to a chemical or environmental stimulus, such as for example IPTG or heat, respectively.

[0111] The present invention further provides, in another aspect, a genetically engineered Gram-negative bacterial strain from which the outer membrane vesicle preparations, of immunogenic compositions or vaccines of the invention which comprise such preparations, can be derived. In one embodiment, the immunogenic composition or vaccine comprises outer membrane vesicle preparations isolated from two or more Gram-negative bacterial strains. In a further embodiment, at least one of said strains belongs to a bacterial species or genus selected from the group consisting of Borrelia; Borrelia burgdorferi; Brucella; Brucella melitensis; Brucella ovis; Chlamydia; Chlamydia psittaci; Chlamydia trachomatis; Escherichia; Escherichia coil; Legionella; Legionella pneumophila; Yersinia; Yersinia enterocolitica; Shigella; Shigella flexneri; Shigella dysenteriae; Shigella boydii; Coxiella; Coxiella burnetii; Acidovorax; Acinetobacter; Acinetobacter baumannii; Acinetobacter calcoaceticus; Acinetobacterjohnsonii; Acinetobacterjunii; Acinetobacter lwoffii; Acinetobacter radioresistens; Actinobacillus; Actinobacillus minor; Actinobacillus pleuropneumoniae; Aggregatibacter; Aggregatibacter actinomycetemcomitans; Aggregatibacter aphrophilus; Alcanivorax; Alcanivorax borkumensis; Azoarcus; Azotobacter; Azotobacter vinelandii; Bordetella; Bordetella bronchiseptica; Bordetella parapertussis; Bordetella pertussis; Bordetella pertussis; Bordetella petrii; Campylobacter; Campylobacter coli; Campylobacter upsaliensis; Comamonas; Comamonas testosteroni; Delftia; Delftia acidovorans; Diaphorobacter; Haemophilus; Haemophilus influenzae; Haemophilus parasuis; Haemophilus somnus; Mannheimia; Mannheimia haemolytica; Moraxella; Moraxella catarrhalis; Neisseria; Neisseria gonorrhoeae; Neisseria meningitidis; Pasteurella; Pasteurella dagmatis; Pasteurella multocida; Proteus; Proteus mirabilis; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas stutzeri; and Sphingomonas.

[0112] In another aspect of the invention is provided a method for producing an immunogenic composition or vaccine comprising: culturing a host cell comprising an expression vector comprising a polynucleotide encoding said antigen under conditions and for a time sufficient for the production of said antigen and recovering the antigen from the culture medium; optionally purifying said antigen by a method selected from the group consisting of ammonium sulphate precipitation, ethanol precipitation, acid extraction, phosphocellulose chromatography, hydrophobic interaction chromatography, hydroxylapatite chromatography and lectin chromatography; and formulating said antigen with a pharmaceutically acceptable carrier or excipient. In the context of such a method the antigen may be endogenous to the host cell, or the host cell may be genetically modified by the introduction of an exogenous antigen. Genetic modification of the host cell for the purposes of producing said antigen include disruption of an endogenous repressor protein, and use of a heterologous and/or strong promoter, as discussed supra.

[0113] The present invention extends to an immunogenic composition or vaccine produced by the provided methods.

[0114] In addition to said antigen, and optionally one or more further antigens, the immunogenic composition or vaccine of the invention may comprise one or more of a pharmaceutically acceptable excipient, an adjuvant and a zinc salt.

[0115] In a further aspect of the invention is provided an immunogenic composition or vaccine comprising at least one polynucleotide encoding an antigen, and optionally an additional antigen, as defined supra, wherein said polynucleotide is operably linked to a eukaryotic promoter. In such an aspect, the antigen itself is not administered, but is produced in vivo following administration of the encoding polynucleotide. Such techniques are known in the art, see for example Wolff et al., Science, (1990) 247: 1465-8. The expression of the antigen in such a polynucleotide would be under the control of a eukaryotic promoter, capable of driving expression within a mammalian cell. The polynucleotide may additionally comprise sequence encoding other antigens. Examples of such eukaryotic promoters include promoters from viruses using mammalian cells as host including adenovirus promoters and retroviral promoters. Alternatively, mammalian promoters could be used.

[0116] The immunogenic composition or vaccine defined supra is, in one embodiment, capable of generating a protective response against infection by a Gram-negative bacterial strain. By a "protective response" as used herein is meant an immune response that prevents, retards the development of, or reduces the severity of a disease that is caused by a Gram-negative bacterial strain, or diminishes or altogether eliminates the symptoms of the disease. In a further embodiment, said composition or vaccine is capable of generating a protective response against infection by two or more different bacterial strains. Suitably, one or more of said different Gram-negative bacterial strains belong to a bacterial genus or species selected from the group consisting of Borrelia; Borrelia burgdorferi; Brucella; Brucella melitensis; Brucella ovis; Chlamydia; Chlamydia psittaci; Chlamydia trachomatis; Escherichia; Escherichia coli; Legionella; Legionella pneumophila; Yersinia; Yersinia enterocolitica; Shigella; Shigella flexneri; Shigella dysenteriae; Shigella boydii; Coxiella; Coxiella burnetii; Acidovorax; Acinetobacter; Acinetobacter baumannii; Acinetobacter calcoaceticus; Acinetobacter johnsonii; Acinetobacter junii; Acinetobacter lwoffii; Acinetobacter radioresistens; Actinobacillus; Actinobacillus minor; Actinobacillus pleuropneumoniae; Aggregatibacter; Aggregatibacter actinomycetemcomitans; Aggregatibacter aphrophilus; Alcanivorax; Alcanivorax borkumensis; Azoarcus; Azotobacter; Azotobacter vinelandii; Bordetella; Bordetella bronchiseptica; Bordetella parapertussis; Bordetella pertussis; Bordetella pertussis; Bordetella petrii; Campylobacter; Campylobacter coli; Campylobacter upsaliensis; Comamonas; Comamonas testosteroni; Delftia; Delftia acidovorans; Diaphorobacter; Haemophilus; Haemophilus influenzae; Haemophilus parasuis; Haemophilus somnus; Mannheimia; Mannheimia haemolytica; Moraxella; Moraxella catarrhalis; Neisseria; Neisseria gonorrhoeae; Neisseria meningitidis; Pasteurella; Pasteurella dagmatis; Pasteurella multocida; Proteus; Proteus mirabilis; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas stutzeri; and Sphingomonas.

[0117] In one aspect, the present invention provides a method for the treatment or prevention of Gram-negative bacterial disease or infection comprising administering a protective dose or an effective amount of an immunogenic composition or vaccine of the invention as defined supra.

[0118] In another aspect is provided an immunogenic composition or vaccine as defined supra for use in the treatment or prevention of Gram-negative bacterial disease or infection.

[0119] By "disease" as used herein means infection by a bacteria or any disease caused by or related to infection by a bacteria including, for example, upper respiratory tract infection and invasive bacterial diseases such as bacteraemia and meningitis.

[0120] Whilst said Gram-negative bacterial disease may be infection, or disease caused, by any Gram-negative bacterial strain, suitably said strain belongs to a bacterial genus or species selected from the group consisting of Borrelia; Borrelia burgdorferi; Brucella; Brucella melitensis; Brucella ovis; Chlamydia; Chlamydia psittaci; Chlamydia trachomatis; Escherichia; Escherichia coil; Legionella; Legionella pneumophila; Yersinia; Yersinia enterocolitica; Shigella; Shigella flexneri; Shigella dysenteriae; Shigella boydii; Coxiella; Coxiella burnetii; Acidovorax; Acinetobacter; Acinetobacter baumannii; Acinetobacter calcoaceticus; Acinetobacter johnsonii; Acinetobacter junii; Acinetobacter lwoffii; Acinetobacter radioresistens; Actinobacillus; Actinobacillus minor; Actinobacillus pleuropneumoniae; Aggregatibacter; Aggregatibacter actinomycetemcomitans; Aggregatibacter aphrophilus; Alcanivorax; Alcanivorax borkumensis; Azoarcus; Azotobacter; Azotobacter vinelandii; Bordetella; Bordetella bronchiseptica; Bordetella parapertussis; Bordetella pertussis; Bordetella pertussis; Bordetella petrii; Campylobacter; Campylobacter coli; Campylobacter upsaliensis; Comamonas; Comamonas testosteroni; Delftia; Delftia acidovorans; Diaphorobacter; Haemophilus; Haemophilus influenzae; Haemophilus parasuis; Haemophilus somnus; Mannheimia; Mannheimia haemolytica; Moraxella; Moraxella catarrhalis; Neisseria; Neisseria gonorrhoeae; Neisseria meningitidis; Pasteurella; Pasteurella dagmatis; Pasteurella multocida; Proteus; Proteus mirabilis; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas stutzeri; and Sphingomonas. In a particular embodiment, optionally said strain is not a Neisserial strain.

[0121] The immunogenic compositions or vaccines of the invention can be administered orally, nasally, nasopharyngeally, parenterally, enterically, gastrically, topically, transdermally, subcutaneously, intramuscularly, in tablet, solid, powdered, liquid, aerosol form, locally or systemically, with or without added excipients. Actual methods for preparing parenterally administrable compositions will be known or apparent to those skilled in the art and are described in more detail in such publications as Remingtons Pharmaceutical Science, 15th ed., Mack Publishing Company, Easton, Pa. (1980).

[0122] It is recognized that oral administration can require protection of the compositions from digestion. This is typically accomplished either by association of the composition with an agent that renders it resistant to acidic and enzymatic hydrolysis or by packaging the composition in an appropriately resistant carrier. Means of protecting from digestion are well known in the art.

[0123] The compositions are administered to an animal that is at risk from acquiring a Gram-negative disease to prevent or at least partially arrest the development of disease and its complications. An amount adequate to accomplish this is defined as a "therapeutically effective dose". Amounts effective for therapeutic use will depend on, for example, the immunogenic composition or vaccine, the manner of administration, the weight and general state of health of the patient, and the judgment of the prescribing physician. Single or multiple doses of the immunogenic compositions or vaccines may be administered depending on the dosage and frequency required and tolerated by the patient, and route of administration.

[0124] The immunogenic compositions or vaccines are administered in an amount effective to elicit an immune response, particularly a humoral immune response, in the host. Amounts for the immunization generally range from about 0.001 mg to about 1.0 mg per 70 kilogram patient, more commonly from about 0.001 mg to about 0.2 mg per 70 kilogram patient. Dosages from 0.001 up to about 10 mg per patient per day may be used, particularly when the antigen is administered to a secluded site and not into the blood stream, such as into a body cavity or into a lumen of an organ. Substantially higher dosages (e.g. 10 to 100 mg or more) are possible in oral, nasal, or topical administration. The initial administration can be followed by booster immunization of the same of different composition, with at least one booster, more usually two boosters, being preferred. The immunogenic compositions or vaccines are typically administered to a mammal that is immunologically naive with respect to a given Gram-negative strain or species. In a particular embodiment, the mammal is a human child about five years or younger, and preferably about two years old or younger, and the antigen compositions are administered at any one or more of the following times: two weeks, one month, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11 months, or one year or 15, 18, or 21 months after birth, or at 2, 3, 4, or 5 years of age. In general, administration to any mammal is preferably initiated prior to the first sign of disease symptoms, or at the first sign of possible or actual exposure to a given Gram-negative strain or species.

[0125] In a further aspect, the present invention provides a method of preparing an immune globulin (for instance for use in prevention or treatment of Gram-negative bacterial disease), comprising the steps of immunising a recipient with an immunogenic composition or vaccine of the invention and isolating immune globulin from the recipient. An immune globulin preparation so obtained, optionally in the form of a pharmaceutical preparation additionally comprising a pharmaceutically acceptable excipient, is also provided.

[0126] Inocula for polyclonal antibody production are typically prepared by dispersing the immunogenic composition or vaccine in a physiologically tolerable diluent such as saline or other adjuvants suitable for human use to form an aqueous composition. An immunostimulatory amount of inoculum is administered to a mammal and the inoculated mammal is then maintained for a time sufficient for the immunogenic composition or vaccine to induce protective antibodies. The antibodies can be isolated to the extent desired by well known techniques such as affinity chromatography. Antibodies can include antiserum preparations from a variety of commonly used animals e.g. goats, primates, donkeys, swine, horses, guinea pigs, rats or man. The animals are bled and serum recovered.

[0127] An immune globulin produced in accordance with the present invention can include whole antibodies, antibody fragments or subfragments. Antibodies can be whole immunoglobulins of any class e.g. IgG, IgM, IgA, IgD or IgE, chimeric antibodies, or hybrid antibodies having dual specificity to a TdfI protein and a TdfH protein. They may also be fragments e.g. F(ab')2, Fab', Fab, Fv and the like including hybrid fragments. An immune globulin also includes natural, synthetic or genetically engineered proteins that act like an antibody by binding to specific antigens to form a complex.

[0128] An immunogenic composition or vaccine of the present invention can be administered to a recipient who then acts as a source of immune globulin, produced in response to challenge from the specific immunogenic composition or vaccine. A subject thus treated would donate plasma from which hyperimmune globulin would be obtained via conventional plasma fractionation methodology. The hyperimmune globulin would be administered to another subject in order to impart resistance against or treat infection by a given Gram-negative bacterial species or strain. Hyperimmune globulins of the invention are particularly useful for treatment or prevention of Gram-negative bacterial disease in infants, immune compromised individuals or where treatment is required and there is no time for the individual to produce antibodies in response to vaccination.

[0129] An additional aspect of the invention is a pharmaceutical preparation comprising monoclonal antibodies reactive against a TdfI protein and/or a TdfH protein, and a pharmaceutically acceptable excipient, which could be used to treat or prevent infection by Gram-negative bacteria. Such pharmaceutical preparations comprise monoclonal antibodies that can be whole immunoglobulins of any class e.g. IgG, IgM, IgA, IgD or IgE, chimeric antibodies, or hybrid antibodies having dual specificity to a TdfI protein and a TdfH protein. They may also be fragments e.g. F(ab')2, Fab', Fab, Fv and the like including hybrid fragments. Methods of making monoclonal antibodies are well known in the art and can include the fusion of splenocytes with myeloma cells (Kohler and Milstein, 1975, Nature 256; 495; Antibodies--a laboratory manual Harlow and Lane 1988). Alternatively, monoclonal Fv fragments can be obtained by screening a suitable phage display library (Vaughan T J et al, 1998, Nature Biotechnology 16; 535). Monoclonal antibodies may also be humanised or part-humanised using techniques that are well-known in the art.

[0130] The present invention extends to a method for treatment or prevention of Gram-negative bacterial disease comprising a step of administering to a patient an effective amount of such a pharmaceutical preparation comprising immune globulin or monoclonal antibodies, and to such preparations for use in the treatment or prevention of such a disease.

[0131] All references or patent applications cited within this patent specification are incorporated by reference herein.

EXAMPLES

[0132] It is understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference in their entirety for all purposes.

Example 1

Immunogenicity of OMVs with Up-Regulation of TdfI

[0133] TdfI is a gene which is thought to be expressed when N. meningitidis is within the blood. It is therefore not normally expressed when strains are grown in conventional culture media, but wild-type strain H44/76, for example, can be made to express the protein in special culture conditions (RPMI culture media supplemented with hemin). The following experiment details the use of an H44/76 strain where TdfI expression has been recombinantly made inducible (through the use of IPTG). This allows the over-expression of TdfI on the surface of OMV vaccines made from the strain, and provides an easy way of culturing a strain expressing the antigen to establish whether antibodies generated against TdfI are capable of killing such a modified strain which expresses TdfI under normal culture conditions (+IPTG). The impact of IPTG on expression of TdfI on cells used in the SBA is shown in FIG. 7.

[0134] Groups of 10 mice were immunized three times with OMV by the intramuscular route on day 0, 21 and 28. Each inoculation was made up of 5 .mu.g (protein content) of OMVs formulated on AIPO4 with MPL. The OMVs were derived from Neisseria meningitidis strain H44/76, engineered so that capsular polysaccharides and PorA were down regulated and LOS immunotype was galE type. A comparison was made of OMVs in which TdfI was or was not up-regulated (up-regulation under the control of IPTG inducible promoter). On day 42, blood samples were taken for analysis by serum bactericidal assay using either the homologous strain H44/76 (B:15:P1.7,16) expressing or not TdfI (after addition or not of IPTG in the culture media).

[0135] N. meningitidis strains were cultivated overnight on GC-agar with 10 .mu.g/ml chloramphenicol Petri Dishes at 37.degree. C.+5% CO.sub.2. They were sub-cultured for 3 hours in a liquid TSB medium supplemented or not with IPTG 1000 .mu.M. Individual sera were inactivated for 30 min at 56.degree. C. Serum samples were diluted in HBSS-BSA 0.5% and then twofold diluted (8 dilutions) in a volume of 25 .mu.l in flat bottom microplates. Bacteria were diluted in HBSS-BSA 0.5% to yield 8.10.sup.3 CFU/ml and 12.5 .mu.l of this dilution was added to the serum dilution. Rabbit complement (12.5 .mu.l) was also added to each well. After 75 min of incubation at 37.degree. C. under shaking, 15 .mu.l of the mixture was spread onto pre-warmed GC-agar plates incubated overnight at 37.degree. C.+CO.sub.2.

[0136] The CFU's were counted and the percentage of killing was calculated. The SBA titer is the dilution giving 50% of killing.

TABLE-US-00006 SBA titers: impact of expression of Tdfl by target cells SBA titers H44/76 without IPTG <50 H44/76 with IPTG 400; 400; 800

[0137] Without IPTG, TdfI is not expressed on target cells which are not killed by sera from mice immunized with up-regulated TdfI OMVs. When the expression of TdfI is specifically induced by IPTG the target cells express TdfI and are killed by anti-TdfI-OMVs mice sera.

Example 2

A Novel Zinc-Regulated Outer Membrane Protein in Neisseria meningitidis with Vaccine Potential

Abstract

[0138] Since the concentration of free iron in the human host is low, efficient iron-acquisition mechanisms constitute important virulence factors for pathogenic bacteria. In the Gram-negative bacteria, TonB-dependent outer membrane receptors are implicated in iron acquisition. However, transport across the bacterial outer membrane of other metals that are also scarce in the human host is far less clear. In this study we characterized a novel TonB-dependent receptor in Neisseria meningitidis. We show that the bacteria produce this protein under zinc limitation and that it is involved in zinc uptake. Furthermore, since the protein is highly conserved among isolates and is capable of inducing bactericidal antibodies, it constitutes a novel candidate for the development of a vaccine against N. meningitidis for which no effective universal vaccine is available so far. Homologues of the protein, designated TfdI, are found in many other pathogens residing in the respiratory tract, suggesting that receptor-mediated zinc uptake is particularly important for survival in this niche.

Introduction

[0139] The cell envelope of Gram-negative bacteria consists of two membranes, the inner and the outer membrane, which are separated by the periplasm containing the peptidoglycan layer. The outer membrane forms a barrier for harmful compounds from the environment. Most nutrients can pass the outer membrane by passive diffusion via abundant channel-forming outer membrane proteins, collectively called porins. However, diffusion is not an option when the extracellular concentration of a nutrient is low. This is the case, for example, for iron. Pathogens are confronted with low concentrations of free iron within the human host, where iron is bound by iron-transport and -storage proteins, such as lactoferrin and transferrin. Hence, efficient iron acquisition mechanisms constitute important virulence factors and have been studied extensively in many pathogens (1, 2).

[0140] When grown under iron-limiting conditions, Gram-negative bacteria induce the synthesis of outer membrane proteins that function as receptors for the iron-binding proteins of the host, for heme, or for siderophores, which are small iron-chelating compounds produced and secreted by the bacteria under iron limitation. The resolved crystal structures of such receptors revealed 22-stranded .beta.-barrels, which do not form open channels but are closed by an N-terminal plug domain (3). After binding of the ligand to the receptor, the subsequent uptake is an active process that requires the energy of the proton gradient across the inner membrane, which is coupled to the receptors in the outer membrane via a complex of three proteins, the TonB complex (4, 5).

[0141] While iron-acquisition mechanisms have been studied extensively in many Gram-negative bacteria, little is known yet about the transport of other essential heavy metals, such as zinc and manganese, across the bacterial outer membrane. The concentration of these trace elements also is low in the human host, which, for example, responds to infections by the production of metallothioneins and calprotectin thereby reducing the availability of metals to the invading pathogens (6, 7). Therefore, Gram-negative pathogens likely possess effective acquisition mechanisms for these metals, which may or may not resemble the iron-acquisition systems.

[0142] Neisseria meningitidis is an obligate human pathogen that can colonize the nasopharyngeal mucosa asymptomatically. Occasionally the bacterium enters the bloodstream and can cause meningitis and sepsis with a high mortality rate (8). While vaccines are available for most pathogenic serogroups of N. meningitidis based on the capsular polysaccharides, a vaccine against serogroup B meningococci is lacking. The polysaccharide capsule of the serogroup B strains is poorly immunogenic due to its resemblance to human glycoproteins (9). Thus, subcapsular antigens are being studied as alternative vaccine components; however, these studies are frustrated by the high antigenic variability of the major outer membrane proteins. Therefore, attention has shifted to minor antigens, including the TonB-dependent receptors.

[0143] When grown under iron limitation, N. meningitidis produces TonB-dependent receptors for lactoferrin (10), transferrin (11), hemoglobin (12, 13) and enterobactin (14), all involved in the uptake of iron. Based on homology searches, Turner et al (15) identified seven additional genes for putative TonB-dependent family (Tdf) members in the available genome sequences of three Neisserial strains. Interestingly, the expression of some of these tdf genes appeared unaffected by iron availability in various microarray studies (16, 17), indicating that their products might be implicated in the transport of metals other than iron. Here we studied the regulation of the synthesis, the function and the vaccine potential of one of these receptors and show that this receptor is involved in the uptake of zinc.

Results

TdfI is not a Heme Receptor

[0144] TdfI (locus tags NMA1161 and NMB0964 in the sequenced genomes of N. meningitidis serogroup A strain Z2491 and serogroup B strain MC58, respectively) was previously identified as one of seven novel putative TonB-dependent receptors present in the Neisserial genomes (15) and was found to be up-regulated in the presence of naive human serum (18). Since almost all TonB-dependent receptors studied to date are involved in iron acquisition we assumed that TdfI transports an iron complex. This idea was strengthened by the fact that blast searches (19) with the amino-acid sequence of NMA1161 revealed high sequence similarity to outer membrane receptors for the uptake of heme, such as HumA of Moraxella catarrhalis (20) with 41% identity and 58% similarity.

[0145] To assess the function of TdfI, we constructed a tdfI deletion mutant of a non-encapsulated derivative of serogroup B strain H44/76 called HB-1. We found similar binding of heme to HB-1 and the tdfI mutant as assessed by dot blot analysis and the tdfI mutant strain could still grow on plates with heme as the sole iron source. We could also not find increased heme binding by Escherichia coli cells expressing TdfI. Also we were unable to complement an E. coli heme auxotroph (data not shown). Therefore, we hypothesized that TdfI, although homologous to heme receptors, does not function as a heme receptor.

Regulation of tdfI by Zinc

[0146] Since TdfI is not a heme receptor and is not found to be regulated by iron, we sought conditions where we could detect tdfI is expression in the capsule deficient H44/76 Neisseia meningitidis HB-1. We could never detect TdfI on Western blots when the bacteria were grown in tryptic soy broth (TSB), a complex rich medium (FIG. 1 A, lane 1). However, when the bacteria were grown in the chemically defined RPMI medium, TdfI was detectable in bacterial lysates (FIG. 1 A, lane 2). The specificity of the signal detected was demonstrated by its absence in the tdfI knockout strain grown in RPMI (FIG. 1 A, lane 3). We noted that the presence of even small amounts of TSB added to RPMI negatively affected TdfI synthesis (FIG. 1 B); apparently TSB contains a compound that represses the transcription of tdfI. Since we noticed that RPMI does not contain a source of trace metals, we decided to test whether addition of a cocktail of trace metals, containing cobalt, molybdenum, manganese, copper and zinc, would repress tdfI expression, which indeed appeared to be the case. We then tested all these metals separately and found that specifically zinc, even at sub-.mu.M concentrations, caused repression of tdfI expression (FIG. 1 C). Since standard RPMI is not supplemented with a specific zinc source, the available zinc required for bacterial growth presumably comes from the water and/or traces in the salts used to make the medium. We measured the zinc concentration in RPMI medium by inductively coupled plasma mass spectrometry (ICP-MS) and found it to be .sup..about.110 parts per billion (.sup..about.1.69 .mu.M).

[0147] The zinc regulation of tdfI became even more evident when we supplemented the RPMI medium with the specific zinc chelator N,N,N',N'-Tetrakis-(2-pyridylmethyl)-Ethylenediamine (TPEN). Addition of TPEN to the medium resulted in a dose-dependent increase in TdfI synthesis (FIG. 1 D). However, concentrations above 1 .mu.M TPEN totally inhibited cell growth presumably due to total zinc depletion from the medium. Growth could be restored by the addition of zinc (data not shown). The zinc regulation of tdfI was confirmed by real-time quantitative PCR (RT-qPCR) using total RNA obtained from cultures grown in RPMI supplemented or not with 500 nM zinc or 0.5 .mu.M TPEN. The data showed a 13.8-fold repression in the presence of zinc and a 3.8-fold up regulation in the presence of TPEN. The fold difference between added TPEN and zinc was 52.6-fold.

Role of the Transcriptional Regulator Zur in tdfI Expression

[0148] In E. coli, the zinc uptake regulator (Zur) has been shown to regulate the expression of the znuACB genes, which encode the periplasmic binding protein, the ATPase and the integral inner membrane component required for zinc transport from the periplasm to the cytoplasm (23). In the presence of zinc, Zur binds a Zur-binding element (consensus GAAATGTTATANTATAACATTTC) in the promoter of the znuACB operon and thereby blocks transcription.

[0149] In the genome sequence of N. meningitidis strain MC58, we identified homologues of the E. coli zur gene, i.e. NMB1266, and of znuCBA, i.e.NMB0588, NMB0587, and NMB0586. In addition, we found sequences resembling the E. coli Zur binding consensus in the regions upstream of the neisserial tdfI (GtAATGTTATATaATAACAaact) and znuC (cAAAcGTTATACagTAtCATaTC) (identical nucleotides to the E. coli consensus are in capital case). To confirm the involvement of Zur in the regulation of tdfI expression, we generated a zur mutant of strain HB-1, which, indeed, produced TdfI constitutively (FIG. 2). Also, RT-qPCR demonstrated the involvement of Zur in the expression of znuA and tdfI as znuA and tdfI expression levels increased 5- and 34-fold, respectively, in the zur mutant compared to its parent strain both grown in the presence of zinc.

TdfI Facilitates Zinc Acquisition

[0150] Since the expression of tdfI is regulated by the availability of zinc, it is likely that TdfI acts as a receptor for zinc or a zinc-containing complex. We first analyzed the amino acid sequence and constructed a topology model of TdfI using the PROFtmb program at www.rostlab.org, (FIG. 3). TdfI contains two cysteine residues in the putative extracellular loop L3. If these cysteines form a disulfide bond (supported by our analysis of the membrane fraction of bacteria by SDS-PAGE with and without DTT where incubation of the sample with the reducing agent resulted in a shift in electrophoretic mobility, presumably due to the disruption of the disulfide bond), they bring two stretches of amino acid residues, both rich in histidine and aspartic acid residues, in close proximity (FIG. 3), which could be of functional importance, since also in the periplasmic ZnuA protein of E. coli, a stretch of His and Asp residues is involved in binding zinc (25). Thus, we considered the possibility that TdfI binds free zinc and transports it to the periplasm. To test this hypothesis we first determined whether TdfI could bind zinc. We compared outer membrane vesicles with and without TdfI for their ability to compete with 4-(2-pyridylazo)resorcinol (PAR) for zinc. The outer membrane vesicles containing TdfI showed .sup..about.40% increased binding of zinc compared to the vesicles without TdfI (FIG. 4A). To test transport of zinc we compared the tdfI knockout, a tonB knockout and their parent strain for the accumulation of intracellular zinc using ICP-MS. HB-1 accumulated .sup..about.33% more zinc than the tdfI mutant or the tonB mutant, indicating that TdfI transports free zinc and that this transport needs the TonB system (FIG. 4B).

[0151] If indeed TdfI is involved in the uptake of free zinc, than one would expect derepression of znu gene expression to occur at higher external zinc concentrations in the tdfI mutant as compared with the wild-type strain. To test this idea, we grew the tdfI mutant and the parent strain in RPMI medium with 500 nM additional zinc, which largely, but not completely represses tdfI expression in the wild-type strain (FIG. 1 C). We subsequently measured the relative levels of tdfI and znuA mRNA by RT-qPCR. The tdfI mutant still contains the first 437 nucleotides of the tdfI gene that were used for the detection of gene expression. In the tdfI mutant, there was 18.6-fold more tdfI and 7.4-fold more znuA expressed, showing that indeed the intracellular zinc concentration in the tdfI mutant is lower than that in the parent strain under the applied growth conditions. Also a znuA knockout strain expressed high levels of TdfI in the presence of zinc, confirming that ZnuA is required to sustain sufficient zinc levels in the cell (FIG. 4C). Thus, both TdfI and ZnuA are involved in the transport of zinc.

Conservation of TdfI

[0152] Besides the function of TdfI we also want to investigate whether TdfI is a vaccine candidate for a universal N. meningitidis vaccine. One of the criteria is that the antigen has to be conserved. We first looked at the available N. meningitidis genomes and found that TdfI has a striking 97-99% amino acid identity of the mature protein (Figure FIG. 8). The sequence differences are scattered throughout the protein and are not clustered in predicted extracellular loop regions, which are often antigenically variable in Neisseria outer membrane proteins (Figure FIG. 8). We subsequently analyzed the presence of TdfI in a panel of 32 different N. meningitidis isolates from different serogroups and different clonal lineages. Each strain was grown in RPMI medium supplemented or not with 500 nM zinc and analyzed by Western blotting with the antiserum raised against TdfI of H44/76. All strains showed a repression of TdfI in the presence of zinc (FIG. 5).

[0153] We then wanted to know the homology of TdfI to other pathogenic bacteria. We first compared TdfI with N. gonorrhea and found a 96% identity and a 97% similarity between these two Neisseria strains. Next, we used the blast program at NCBI with a cutoff of 40% identity at the amino acid level to search for homologs of TdfI in other pathogenic bacteria. We identified homologs in other pathogenic bacteria, including M. catarrhalis, Haemophilus parasuis, Mannheimia haemolytica, Acinetobacter baumannii, Pasteurella multocida, Bordetella pertussis and Actinobacillus pleuropneumoniae, averaging a 41% identity and 59% similarity at the amino acid level and all TdfI homologs have the His/Asp region (FIG. 9). Interestingly, in B. pertussis the tdfI homologue is located adjacent to homologues of the znuABC and zur genes, again indicating a functional relationship between these genes. Furthermore, all these TdfI homologs contain His- and Asp-rich stretches (FIG. 9).

TdfI Induces Bactericidal Antibodies

[0154] To investigate the vaccine potential of TdfI, we immunized mice with Neisserial outer membrane vesicles containing overexpression levels of this protein (FIG. 6A) and tested the resultant sera for the presence of bactericidal antibodies. Routinely, we perform serum bactericidal assays on bacteria grown in TSB medium; however, under these conditions tdfI is not expressed. Therefore, we tested the sera for bactericidal activity on a strain that expressed TdfI from an isopropyl .beta.-D-1-thiogalactopyranoside (IPTG)-inducible promoter and compared cultures grown with and without IPTG. The bactericidal titers of the sera were <1:100 when IPTG was absent, but 1:1042 when IPTG was present during growth of the bacteria. Titers in pre-immune sera were also <1:100. These data clearly show that TdfI is able to elicit bactericidal antibodies. We also wanted to investigate whether normal chromosome-encoded tdfI expression levels are sufficient to mediate complement-mediated killing. For this we employed the zur knockout strain that produces TdfI constitutively in the TSB medium and grows comparable to the wild-type strain in this medium.

Discussion

[0155] The high-affinity ZnuABC uptake system for zinc has previously been identified in N. gonorrhoeae (30). Homologues can be found in the meningococcal genome, as described above, and in the genomes of many other bacteria. In Salmonella enterica this ABC transporter has been associated with virulence (31). In no case, an outer membrane receptor involved in zinc acquisition has been identified and it is thought that zinc diffuses through the porins.

[0156] In the human host, however, the free zinc levels are most likely too low to sustain bacterial growth by passive diffusion. The total amount of zinc in human serum is approximately 19 .mu.M, but the vast majority is bound by serum proteins such as albumin (32). Here we have identified an outer membrane receptor, TdfI that is regulated by zinc. The addition of 700 nM zinc to the growth medium completely repressed TdfI expression. The function of TdfI is to bind and transport of unbound (free) zinc. We predict that the zinc is bound initially by the His/Asp stretch in the external loop and then internalized via two histidines that are on top of the plug domain (FIG. 3b). A possible role for the TonB system in zinc uptake is that it pulls the plug out of the barrel and with this movement the zinc bound to the two His residues is transported into the periplasm where it is picked up by the periplasmic binding protein ZnuA.

[0157] Interestingly, similar regulation of tdfI and znuA expression was reported in a microarray study using N. gonorrhoeae (33). The tdfI homolog NG01205 and the znuA homolog NG00168 were upregulated in a mutant lacking the NG00542 gene. This gene was annotated in that study as perR because of its homology to a manganese-dependent peroxide-responsive regulator found in gram-positive organisms (34). However, this is the same gene we have annotated as zur. The zur annotation is clearly more accurate, because we show an identical regulation by the absence of zur or the absence of zinc. More evidence for the annotation zur rather than perR comes from the same study in N. gonorrhoeae. Microarrays performed with the gonococcal perR mutant showed upregulation also of the ribosomal proteins L31 and L36. The Neisserial genomes contain two copies for each of the genes encoding these proteins with one form of each protein containing a zinc ribbon motif. Zinc availability was found to be the key factor controlling the type of L31/L36 protein expressed in B subtilis (34). In the gonococcal perR mutant, expression specifically of L31 and L36 paralogs lacking the zinc ribbons is induced, highly indicative of a disturbed zinc regulation in a perR mutant. Moreover in another study (17) a microarray was performed to identify the response to oxidative stress and neither perR nor any of the genes identified in the PerR study (33) were de-repressed and we do not see any regulatory effect of manganese on the expression of tdfI and znuA.

[0158] Previously, tdfI expression was reported to be induced in the presence of active complement (18). In this microarray study expression profiles were compared of N. meningitidis grown in the presence of serum and heat-inactivated serum, and TdfI was found 23-fold de-repressed in the presence of the untreated serum. The relationship between zinc and complement regulation may not be obvious at first sight. A possible explanation for finding similar regulatory circuits may be that the bacteria in the array study were pre-grown in RMPI with BSA. Albumin is known to chelate zinc, and therefore, pre-growth conditions may have been severely zinc-limited. Heat-treatment of human serum will release zinc from albumin, thereby repressing tdfI expression. This explanation is strengthened by the fact that TdfI expression is induced when BSA is added to TSB medium during bacterial growth (data not shown).

[0159] A study by Hagen and Cornelissen (35) investigated whether any of the Tdf proteins is essential for intracellular survival of N. gonorrhoeae in human epithelial cells. The authors also tested a TdfI homologue knockout (NG1205), but this mutant was not affected in the intracellular survival.

[0160] The conservation of TdfI is striking; with an identity of 98.6% among the sequenced N. meningitidis strains and a 99.2% similarity at the amino acid level of the mature protein. The TdfI protein was found in all meningococci tested and all strains showed zinc-regulated expression of tdfI. Between the TdfI proteins of the sequenced meningococcal and gonococcal strains there is 96.1% identity and 97.3% similarity at the amino acid level. The differences between the sequences of TdfI are scattered throughout the protein and do not cluster in a specific loop. We find an average 41% amino acid identity of TdfI with homologs in other bacteria and in all cases the His/Asp stretch is conserved. Intriguingly, TdfI homologs were particularly found in bacterial species residing in the respiratory tract of humans and animals. Possibly in the mucosal layers of the respiratory tract the unbound zinc concentration is too low to allow sufficient passive diffusion through the porins and therefore TdfI becomes essential for bacterial growth and survival. While TdfI is not essential for intracellular survival (35) it could be essential in the bodily fluids like serum and liquor where the free zinc concentration could also be very low. Also, we cannot rule out that TdfI additionally recognizes a complexed form of zinc which may available in the respiratory tract, serum and or cerebral fluid.

[0161] We have further shown that TdfI can induce bactericidal antibodies in mice and that these antibodies are specifically directed at TdfI. Also when we used bacteria expressing TdfI from the chromosomal locus we could detect bactericidal activity, showing that during infection the antigen concentration is high enough to allow clearing of N. meningitidis.

[0162] The high level of conservation and the possibility to raise TdfI-specific bactericidal antibodies make TdfI an excellent vaccine candidate.

Materials and Methods

[0163] Abbreviations used: IPTG, isopropyl .beta.-D-1-thiogalactopyranoside; PAR, 4-(2-pyridylazo)resorcinol; RPMI, Roswell Park Memorial Institute medium 1640; Tdf, TonB-dependent family; TPEN, N,N,N',N'-Tetrakis(2-pyridylmethyl)ethylenediamine; TSB, tryptic soy broth; ICP-MS, Inductively coupled plasma mass spectrometry.

Bacterial Strains and Growth Conditions.

[0164] Neisserial strains, listed in FIG. 5 are from the laboratory collection. Except when indicated otherwise, experiments were performed with strain HB-1 and mutants thereof. HB-1 is a non-encapsulated derivative of serogroup B strain H44/76 (Bos & Tommassen, 2005). N. meningitidis was grown on GC agar (Oxoid) plates containing Vitox (Oxoid) and antibiotics when appropriate (kanamycin, 100 .mu.g/ml; chloramphenicol, 10 .mu.g/ml) in candle jars at 37.degree. C. Liquid cultures were grown in TSB (Difco) or in RPMI (Sigma) in plastic flasks at 37.degree. C. with shaking. IPTG, zinc, and TPEN were added in the concentrations indicated s. Metals were added as a cocktail (340 nM ZnSO.sub.4, 160 nM Na.sub.2MoO.sub.4, 800 nM MnCl.sub.2, 80 nM CoCl.sub.2 and 80 nM CuSO.sub.4 final concentrations) or as single compounds in the same concentrations as in the cocktail unless indicated otherwise. Ferric chloride was added as a final concentration of 8 .mu.M. E. coli strains DH5.alpha. and TOP10F' (Invitrogen) were used for routine cloning and BL21(DE3) (Invitrogen) for expression. An E. coli hemA mutant was used to assess the heme transport of TdfI ((22). E. coli was propagated on Luria-Bertani medium supplemented when appropriate with 100 .mu.g/mlampicillin, 50 .mu.g/ml kanamycin, or 25 .mu.g/ml chloramphenicol. For the E. coli heme-auxotroph C600 hemA::kan (22) the medium was supplemented with 5-aminolevulinic acid.

Construction of Plasmids and Mutants.

[0165] All primers were designed on the MC58 genome sequence, using NMB0964 (tdfI), NMB1730 (tong), NMN0586 (znuA), NMB1266 (zur).

[0166] For high-level protein production in E. coli the tdfI gene without the signal sequence-encoding part was amplified from chromosomal DNA of strain H44/76 by PCR using the primers 0964-F-GATCATATGCATGAAACTGAGCAATCGGTG- and 0964-R-GATGGATCCTTAAATCTTCACGTTCACGCCGCC- that carry the restriction sites NdeI and BamHI, respectively (bold). The resulting product was cloned into pCRII-TOPO according to the manufacturer's recommendation (Invitrogen), yielding pCRII-tdfI, and subcloned into pET11a (Novagen) using NdeI/BamHI restriction, resulting in plasmid pET11a-tdfI.

[0167] To obtain a tdfI deletion construct, a kanamycin-resistance gene cassette (36) was amplified by PCR with the primers Kan-R-TGACGCGTCTCGACGCTGAGGTCTGC- and Kan-F-TGTGTACAGTCGACTTCAGACGGCCACG- and cloned after Mlul and BsrGI digestion into pCRII-tdfI digested with the same enzymes. In the resulting construct, pCRII-tdfI::kan, the kanamycin-resistance cassette substitutes for the region between by 437 and 1344 of tdfI. pCRII-tdfI::kan was used in a PCR with the 0964-R and 0964-F primers and the resulting product was used to transform HB-1 (37). Kanamycin-resistant colonies were tested for correct gene replacement by PCR.

[0168] The entire tdfI gene from H44/76 was amplified with primers TdfI-F-GCATCATATGGCACAAACTACACTCAAACCC- and TdfI-R-ATGACGTCTTAAAACTTCACGTTCACGCCGCC- that contain recognition sites for NdeI and AatII (bold), respectively. The resulting PCR product was cloned into pCRII-TOPO and subcloned into pEN11-pldA (36) using NdeI and AatII restriction sites. The resulting plasmid, pEN11-tdfI, constitutes a Neisserial replicative plasmid, containing a lacI.sup.Q gene and a tandem lac/tac promoter for controlled expression of tdfI.

[0169] The construct to generate a tonB knockout was made by amplifying DNA fragments upstream and downstream of the tonB gene using primers tonB-1 (GTACGATGATTGTGCCGACC), tonB-2 (ACTTTAAACTCCGTCGACGCAAGTCGACTGCGGGGGTTAA) with AccI restriction sites (bold) for one fragment, and, tonB-3 (TTAACCCCCGCAGTCGACTTGCGTCGACGGAGTTTAAAGT) with restriction site AccI (bold) and tonB-4 (GCCATACTGTTGCGGATTTGA) for the other fragment. The two fragments were each cloned into pCRII-TOPO and then ligated to each other using the introduced restriction site AccI and the SpeI site in the pCRII-TOPO vector. The AccI site was subsequently used to clone the chloramphenicol transacetylase gene from pKD3 (38) previously cloned into pCRII-TOPO by PCR amplification with primers containing an AccI site. The resulting construct was amplified by PCR using primers tonB-1 and tonB-4 and this linear fragment was used to transform N. meningitidis HB-1.

[0170] The zur gene was knocked out following the same strategy. Upstream and downstream fragments were amplified in this case with primers: zur-1 (TTCGCCGATGGCGGAATACA), zur-2 (CTTTCAGCGCAAAGTCGACTCCGTCGACGCGTGCCTGTTC) with the restriction site AccI in bold, zur-3 (GAACAGGCACGCGTCGACGGAGTCGACTTTGCGCTGAAAG) with the restriction site AccI in bold and zur-4 (TCCTATTGCGCAATACCCCC)

[0171] A porA derivative of N. meningiditis strain H44/76, called CE2001 (39) was transformed with pMF121, resulting in deletion of the entire capsule locus and production of lipopolysaccharide with a truncated outer core (36). A pLAFR-derived plasmid containing the tonB, exbB and exbD genes of N. meningitidis ((13) was described previously.

SDS-PAGE and Western Blot Analysis.

[0172] Cell lysates were prepared from bacteria grown for 6 hours. The cells were diluted to 0 D.sub.600, 1, pelleted, and boiled in 100 .mu.l SDS-PAGE sample buffer containing 2% SDS and 5% 2-mercaptoethanol. Proteins were separated by standard SDS-PAGE. Gels were either stained with Coomassie brilliant blue or the proteins were transferred to nitrocellulose membranes (Protran) using a wet transfer system (Biorad) in 25 mM Tris-HCl, 192 mM glycine, 20% methanol. Membranes were blocked for 1 h in PBS containing 0.1% Tween 20 and 0.5% Protifar (Nutricia). Blots were incubated with antibodies in blocking buffer. Antibody binding was detected by using goat anti-rabbit IgG peroxidase-conjugated secondary antibodies (Biosource) and enhanced chemiluminescence detection (Pierce).

Immunizations.

[0173] BL21(DE3) cells containing pET11a-tdfI were grown in LB to an OD A.sub.600 of 0.6 after which 1 mM IPTG was added and growth was continued for 2 h. The TdfI protein accumulated in inclusion bodies, which were isolated as described (40), and the purified protein was used to immunize rabbits at Eurogentec. The resulting antiserum, SN1042, was used in a 1/5000 dilution.

[0174] Outer membrane vesicles of strain CE1523/pEN11-tdfI grown in the presence or absence of 1 mM IPTG, were prepared by deoxycholate extraction (41) and used to immunize mice as described (32). Sera from ten mice per group were collected after 42 days and pooled. The experiments complied with the relevant national guidelines of Belgium and institutional policies of GlaxoSmithKline Biologicals.

RT-qPCR.

[0175] RT-qPCR was performed using an Applied Biosystems 7900HT Fast Real-Time PCR System and SYBR green master mix (Applied Biosystems) according to the manufacturer's recommendations. Total RNA was isolated by resuspending approximately 4.times.10.sup.9 Neisseria cells in 3 ml Trizol (Invitrogen). After the addition of 600 .mu.l chloroform and centrifugation, the upper phase was mixed 1:1 with 75% ethanol. This was loaded on a nucleospin RNA II column (Macherey-Nagel), which was subsequently washed with buffer R3 from the nucleospin RNA II kit and eluted with 100 .mu.l water. The RNA was then treated with Turbo DNA Free (Ambion) to yield DNA-free RNA. To generate the cDNA, 1 .mu.g of total RNA was reverse transcribed from random hexamers using transcriptor High fidelity cDNA synthesis kit (Roche) according to the manufacturer's recommendations. As a control, parallel samples were prepared in which the reverse transcriptase was omitted from the reaction mixture. PCRs were performed in triplicate in a 25-.mu.l volume in a 96-well plate (Applied Biosystems) with the following cycle parameters: 95.degree. C. for 10 min for enzyme activation followed by 40 cycles of 95.degree. C. for 15 s and 60.degree. C. for 1 min. A melting plot was performed to ensure that the signal originated from the specific amplicon. Data analysis was performed using the comparative cycle threshold method (Applied Biosystems) to determine relative expression levels. The rmpM transcript was used to normalize all data.

ICP-MS.

[0176] Total zinc concentrations were measured by ICP-MS at the integrated laboratory of the department of Geochemistry at the Utrecht University. N. meningitidis strains were grown in RPMI medium from a 0.1 starting OD A.sub.550 for 6 h; at this time point a sample was taken and the remaining culture was grown for an additional hour in the presence of 1 .mu.M zinc. After this hour, a second sample was taken. Both samples (7 ml) were washed in phosphate-buffered saline and resuspended in water, killed for 1 h at 56.degree. C. and frozen at -80.degree. C. The samples were then thawed, sonicated and filtered through 0.22-.mu.m filters (Millipore).

PAR Competition Assay.

[0177] The PAR competition assay is a colorimetric reaction where the orange color of the PAR-zinc complex changes towards yellow in the presence of a protein or chemical that is able to release zinc from PAR. The assay was performed as described (42) with the following modifications: Instead of 50 .mu.M we added 30 .mu.M zinc and we first measured the PAR-zinc solution and then added the outer membrane vesicles to the cuvette and re-measured the solution. In this way we avoided the potential color change induced in time by UV. The data was then first normalized to the PAR-zinc measurement and then to the PAR alone sample to obtain the binding values for the outer membrane vesicles. The results shown are the normalized data of the absorption at 500 nm.

Serum Bactericidal Assay.

[0178] Wild-type H44/76 was transformed with pEN11-tdfI and inoculated from overnight grown plates in TSB with 125 .mu.M FeCl.sub.3 with or without 1 mM IPTG in shaking flasks for 3 h at 37.degree. C. until an OD A.sub.550 of 0.5 was reached. Serum to be tested was diluted 1:100 in Hank's balanced salt solution (HBSS) (GIBCO), 0.3% BSA and then serially diluted (two-fold dilution steps, eight dilutions) in a 50-.mu.l volume in sterile U-bottom 96-well microtiter plates (NUNC). Bacteria were diluted in HBSS, 0.3% BSA to yield .sup..about.13,000 CFU per ml and 37.5 .mu.l samples of the suspension were added to the serum dilutions. The microtiter plates were incubated at 37.degree. C. for 15 min while shaking. Subsequently, 12.5 .mu.l of baby-rabbit complement (Pelfreez) or, as control for toxicity of the sera, heat-inactivated (56.degree. C. for 45 min) complement was added to the wells. After 1 h incubation at 37.degree. C. while shaking, the microtiter plates were placed on ice to stop the killing. Of each well, 20 .mu.l was spotted on GC plates while plates were tilted to allow the drop to "run" down the plate. After overnight incubation, colonies were counted and the percentage of killing was calculated. The bactericidal titer was defined as the highest serum dilution yielding >50% killing.

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[0205] 27. Dempsey, J. A., W. Litaker, A. Madhure, T. L. Snodgrass, and J. G. Cannon. 1991. Physical map of the chromosome of Neisseria gonorrhoeae FA1090 with locations of genetic markers, including opa and pil genes. J. Bacteriol. 173:5476-5486.

[0206] 28. Parkhill, J., M. Achtman, K. D. James, S. D. Bentley, C. Churcher, S. R. Klee, G. Morelli, D. Basham, D. Brown, T. Chillingworth, R. M. Davies, P. Davis, K. Devlin, T. Feltwell, N. Hamlin, S. Holroyd, K. Jagels, S. Leather, S. Moule, K. Mungall, M. A. Quail, M. A. Rajandream, K. M. Rutherford, M. Simmonds, J. Skelton, S. Whitehead, B. G. Spratt, and B. G. Barrell. 2000. Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature. 404:502-506.

[0207] 29. Tettelin, H., N. J. Saunders, J. Heidelberg, A. C. Jeffries, K. E. Nelson, J. A. Eisen, K. A. Ketchum, D. W. Hood, J. F. Peden, R. J. Dodson, W. C. Nelson, M. L. Gwinn, R. DeBoy, J. D. Peterson, E. K. Hickey, D. H. Haft, S. L. Salzberg, O. White, R. D. Fleischmann, B. A. Dougherty, T. Mason, A. Ciecko, D. S. Parksey, E. Blair, H. Cittone, E. B. Clark, M. D. Cotton, T. R. Utterback, H. Khouri, H. Qin, J. Vamathevan, J. Gill, V. Scarlato, V. Masignani, M. Pizza, G. Grandi, L. Sun, H. O. Smith, C. M. Fraser, E. R. Moxon, R. Rappuoli, and J. C. Venter. 2000. Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science. 287:1809-1815.

[0208] 30. Chen, C. Y., and S. A. Morse. 2001. Identification and characterization of a high-affinity zinc uptake system in Neisseria gonorrhoeae. FEMS Microbiol. Lett. 202:67-71.

[0209] 31. Ammendola, S., P. Pasquali, C. Pistoia, P. Petrucci, P. Petrarca, G. Rotilio, and A. Battistoni. 2007. The high affinity Zn.sup.2+ uptake system ZnuABC is required for bacterial zinc homeostasis in intracellular environments and contributes to virulence of Salmonella enterica. Infect. Immun. 75:5867-5876.

[0210] 32. Stewart, A. J., C. A. Blindauer, S. Berezenko, D. Sleep, and P. J. Sadler. 2003. Interdomain zinc site on human albumin. Proc. Natl. Acad. Sci. USA. 100:3701-3706.

[0211] 33. Wu, H. J., K. L. Seib, Y. N. Srikhanta, S. P. Kidd, J. L. Edwards, T. L. Maguire, S. M. Grimmond, M. A. Apicella, A. G. McEwan, and M. P. Jennings. 2006. PerR controls Mn-dependent resistance to oxidative stress in Neisseria gonorrhoeae. Mol. Microbiol. 60:401-416.

[0212] 34. Nanamiya, H., G. Akanuma, Y. Natori, R. Murayama, S. Kosono, T. Kudo, K. Kobayashi, N. Ogasawara, S. M. Park, K. Ochi, and F. Kawamura. 2004. Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome. Mol. Microbiol. 52:273-283.

[0213] 35. Hagen, T. A., and C. N. Cornelissen. 2006. Neisseria gonorrhoeae requires expression of TonB and the putative transporter TdfF to replicate within cervical epithelial cells. Mol. Microbial. 62:1144-1157.

[0214] 36. Bos, M. P., B. Tefsen, P. Voet, V. Weynants, J. P. M. van Putten, and J. Tommassen. 2005. Function of neisserial outer membrane phospholipase A in autolysis and assessment of its vaccine potential. Infect. Immun. 73:2222-2231.

[0215] 37. Voulhoux, R., M. P. Bos, J. Geurtsen, M. Mols, and J. Tommassen. 2003. Role of a highly conserved bacterial protein in outer membrane protein assembly. Science. 299:262-265.

[0216] 38. Datsenko, K. A., and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA. 97:6640-6645.

[0217] 39. Tommassen, J., P. Vermeij, M. Struyve, R. Benz, and J. T. Poolman. 1990. Isolation of Neisseria meningitidis mutants deficient in class 1 (PorA) and class 3 (PorB) outer membrane proteins. Infect. Immun. 58:1355-1359.

[0218] 40. Dekker, N., K. Merck, J. Tommassen, and H. M. Verheij. 1995. In vitro folding of Escherichia coli outer-membrane phospholipase A. Eur. J. Biochem. 232:214-219.

[0219] 41. Weynants, V. E., C. M. Feron, K. K. Goraj, M. P. Bos, P. A. Denoel, V. G. Verlant, J. Tommassen, I. R. Peak, R. C. Judd, M. P. Jennings, and J. T. Poolman. 2007. Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidis. Infect. Immun. 75:5434-5442.

[0220] 42. Lim, K. H., C. E. Jones, R. N. vanden Hoven, J. L. Edwards, M. L. Falsetta, M. A. Apicella, M. P. Jennings, and A. G. McEwan. 2008. Metal binding specificity of the MntABC permease of Neisseria gonorrhoeae and its influence on bacterial growth and interaction with cervical epithelial cells. Infect. Immun. 76:3569-3576.

TABLE-US-00007

[0220] TABLE 1 Conservation of the mature Tdfl protein sequence in the sequenced Neisseria strains. Identity (%) NCCP Strain MC58 Fam18 Z2491 053442 FA1090 11945 ST-640 Similarity (%) N. 730/734 720/734 720/734 706/734 707/734 712/734 meningitidis (99.5) (98.1) (98.1) (96.2) (96.3) (97.0) MC58 N. 733/734 722/734 718/734 705/734 706/734 712/734 meningitidis (99.9) (98.4) (97.8) (96.0) (96.2) (97.0) Fam18 N. 725/734 726/734 716/734 707/734 706/734 710/734 meningitidis (98.8) (98.9) (97.5) (96.3) (96.2) (96.7) Z2491 N. 726/734 727/734 723/734 706/734 707/734 707/734 meningitidis (98.9) (99.0) (98.5) (96.2) (96.3) (96.3) 053442 N. 715/734 714/734 714/734 715/734 733/734 702/734 gonorrhoeae (97.4) (97.3) (97.3) (97.4) (99.9) (95.6) FA1090 N. 716/734 715/734 713/734 716/734 733/734 701/734 gonorrhoeae (97.5) (97.4) (97.1) (97.5) (99.9) (95.5) NCCP11945 N. 717/734 718/734 718/734 715/734 711/734 710/734 lactamica (97.7) (97.8) (97.8) (97.4) (96.9) (96.7) ST-640

Example 3

ZnuD: A Potential Vaccine Candidate for a Simple and Universal Neisseria meningitidis Vaccine

ABSTRACT

[0221] Neisseria meningitidis serogroup B is a major cause of bacterial sepsis and meningitis in younger populations. Available vaccines are based on outer membrane vesicles obtained from wild type strains. However, in toddlers and infants, they confer protection only against strains expressing homologous PorA, a major and variable outer membrane protein. In the quest to identify vaccine antigens allowing the development of vaccines able to prevent meningococcal infection in the younger populations, ZnuD (TdfI) has been identified as a potential candidate. Here, we have extended the analysis of the potential value of ZnuD showing that it is a very well conserved antigen expressed by all the N. meningitidis serogroup B strains tested when growing under Zinc limitation, induces cross-bactericidal antibodies against most of the strains tested, whatever the serogroup, and is also expressed during infection and most probably during carriage. In addition, anti-ZnuD antibodies are able to mediate the complement killing of strains not killed by anti-human factor H binding protein (fHbp).

INTRODUCTION

[0222] Neisseria meningitidis is a gram negative, encapsulated bacterium. It is an obligate human pathogen that asymptomatically colonizes the upper respiratory tract of around 10% of the adult population. Occasionally, it translocates to the bloodstream resulting in bacteremia with possible progression to meningitis and death. N. meningitidis is one of the most feared bacterial infections due to its rapid progression and tendency to cause epidemics. This bacterium is classified into 13 serogroups on the basis of the chemical composition of the capsular polysaccharides. However, only serogroups A, B, C, Y, and W-135, and to a minor extent X, have been associated with disease. Conjugate polysaccharide vaccines that provide effective immunity in humans are becoming available for serogroups A, C, Y and W135. Unfortunately, the conjugate approach cannot be easily applied to serogroup B (MenB) because its capsular polysaccharide shares structural similarity with polysialylated host glycoproteins, such as the neural cell adhesion molecule.

[0223] First generation of MenB vaccines were based on outer membrane vesicles (OMVs) purified after detergent extraction to reduce the lipooligosaccharide (LOS) content. PorA is one of the most abundant outer membrane proteins (OMPs) and an immunodominant component in these OMVs. However, PorA displays high antigenic variability limiting the efficacy of OMV-based vaccines, especially below 2-4 years of age; an age group which totalized around 50% of MenB cases in Europe with an incidence of around 16/100,000 babies below 1 year old. To overcome this limitation, the use of conserved minor OMPs has been explored

[0224] Recently, the potential of an integral OMPs has vaccine candidate was recognised. This protein, named ZnuD for zinc uptake component D, is expressed under zinc limitation and is involved in zinc uptake. Based on the potential of this protein as vaccine candidates, we have extended its study, looking for the presence of anti-ZnuD antibodies in human, its potential to induce cross-bactericidal antibodies and confirming that this protein is very well conserved and expressed all the N. meningitidis strains tested, whatever the serogroup, with the exception of few serogroup Y strains from ST-23 clonal complex.

Results

ZnuD is Immunogenic and Expressed in Humans.

[0225] ZnuD is not expressed by N. meningitidis growing on classical agar culture media except after addition of zinc chelator such as TPEN, questioning its expression in the host. An indirect way to demonstrate that ZnuD is expressed in human is to evaluate the presence of anti-ZnuD antibodies in sera from convalescent patients and/or healthy carriers. For this purpose, a peptide array approach was used covering 77% of the non-mature protein.

[0226] Sera from 12 convalescent subjects and from 11 carriers were tested 3 to 5 times in this peptide array. The FIG. 10 shows the intensity of the individual responses between the two groups of subjects. Both the intensity of the response against the peptides and the number of peptides recognized by the sera were greater in the convalescent group than in the carrier group. All the convalescent sera reacted at least against the same 5 non-consecutive peptides indicating that ZnuD is clearly expressed during infection. Presence of antibodies in carrier sera reacting against some peptides also suggests that ZnuD is expressed during colonization of the upper-respiratory tract.

Development of Relevant Culture Conditions to Assess the Vaccine Potential of ZnuD

[0227] As has previously been demonstrated, anti-ZnuD antibodies are able to mediate the complement killing of its homologous strain genetically modified to over-express ZnuD. The evaluation of the cross-bactericidal potential of ZnuD antibodies by using a large panel of strains does not allow to modify genetically all these strains. Because, the expression of ZnuD is regulated by Zur, we have defined the amount of zinc chelator needed to achieve the same level of ZnuD expression by a wild type strain than a delta zur strain. This was achieved by using 20 .mu.M of TPEN in MH agar plates (FIG. 11).

ZnuD is a Very Well Conserved Antigen and Expressed by all Neisseria meningitidis Strains Except Some German Serogroup Y Strains from 5T23 Clonal Complex.

[0228] It has already been demonstrated that ZnuD is expressed by all strains but one on a panel of 132 strains from serogroups A, B, C, W-135 and Y. This was extended and confirmed for 43 additional strains, including 2 strains of each serogroup A, C, W-135 and Y and 30 serogroup B strains isolated since 2005 from convalescent subjects located in United Kingdom, Spain and Germany. Globally, of the 175 strains tested, only one did not express ZnuD. This strain is a serogroup Y from ST-23 clonal complex isolated in a German patient.

[0229] Particular attention was paid to serogroup Y strains from this ST-23 clonal complex. Seven strains, isolated in Germany were tested and all did not express ZnuD. Sequence analysis has revealed for 4 strains a stop mutation due to single nucleotide deletion. Three of them had the deletion at the same position.

Znu D Induces Cross-Bactericidal Antibodies Against Serogroups A, B, C, W-135 and Y

[0230] The demonstration of the expression and immunogenicity of ZnuD in humans prompted us to more deeply evaluate the potential of ZnuD as a universal N. meningitidis vaccine antigen. For this purpose, OMVs were produced from two strains over-expressing ZnuD or not. These two strains have a similar background since both are derived from H44/76 strain which express an fHbp family B. To avoid the presence of residual capsular polysaccharide in OMV preparations and the induction of anti-LOS and PorA-specific bactericidal antibodies, galE and porA deletion mutations were performed in both strains.

[0231] SDS-PAGE analysis suggested that ZnuD represents around 15% of the protein content of OMVs produced from the over-expressing strains while ZnuD is not observed in the control OMVs preparations (FIG. 12a). Because fHbp was described to be very immunogenic when presented in OMVs, its content was checked by ELISA. It was estimated that the amount of fHbp in ZnuD OMVs was 4 fold higher than in the control OMVs (FIG. 12b). Mice and guinea-pigs were immunized three times with OMVs adsorbed on AIPO4. Serum samples were obtained 2 weeks after the third immunization and they were pooled in order to get 3 pools of sera per groups.

[0232] ELISA was used to assess the response against both ZnuD and fHbp (Table 3). Immunisation with control OMVs induced few or no anti-ZnuD antibodies in both mice and guinea-pigs (GMT=73 and 10 EU/ml, respectively). As expected, high level of anti-ZnuD antibodies were measured in the sera from animals immunized with ZnuD OMVs (GMT=15096 and 5893 EU/ml, respectively). Surprisingly, the immunogenicity of fHbp appears to be different between mice and guinea-pigs. The level of anti-fHbp antibodies in sera from guinea-pig immunized with either control or ZnuD OMVs are clearly lower (335 and 2733 EU/ml, respectively) compared to mice sera (1342 and 59314 EU/ml, respectively). The different level of anti-fHbp antibodies between anti-control OMV and anti-ZnuD OMV sera is in line with the different amount of fHbp in control and ZnuD OMVs.

[0233] Pooled sera were tested in SBA against a panel of 14 serogroup B N. meningitidis strains cultivated with or without TPEN. Six of these strains are regularly used by the HPA for the evaluation of MenB vaccine. This panel include two epidemic strains (H44/76 and NZ98/254) as well as 4 strains of the 4 most prevalent United Kingdom serosubtypes which were accountable for 59.3% of the MenB disease in 2000 and 2001 (M01-240013, M01-240101, M01-240149 and M01-240355). The expression of ZnuD, in presence of TPEN, was confirmed by Western-blot analysis for all these 14 strains. In addition, and due to the presence of anti-fHbp antibodies in sera from animals immunized with OMVs, a pool of sera from mice immunized with a recombinant fHbp B was also tested in bactericidal assay (Table 3).

[0234] The bactericidal activity of anti-fHbp antibodies is related to the level of expression of fHbp by the bacteria and is family specific. Using classical culture conditions, only 5 strains, expressing medium (+/-) to high level (++) of the fHbp B, were killed by anti-fHbp B sera (cut-off of positivity fixed at 128), while none of strains expressing either fHbp A or low level of fHbp B were killed. In presence of TPEN, the same 5 strains plus two (NZ124 and DE10690-06) are killed by anti-fHbp B antibodies. SBA titers, obtained in presence or absence of TPEN, are globally similar with a trend to measure slightly higher titers in presence of TPEN. This suggests that the sensitivity of strains to the complement killing mediated by antibodies is not dramatically increased in presence of the chelator.

[0235] Sera from mice immunized with control OMVs were not able to mediate the complement killing of strains when cultivated in absence of TPEN. In presence of the chelator, only three strains displayed SBA titers just above the cut-off. As these 3 strains were killed by anti-fHbp B antibodies, the killing observed with anti-control OMVs sera is most probably due to the presence of anti-fHbp antibodies and a slightly increased sensitivity of the strains to complement killing when cultivated with TPEN. The role of these anti-fHbp antibodies was clearly demonstrated for one of these 3 strains by performing bactericidal assay with a .DELTA.fHbp H44/76 strain (Table 4). Absence of killing by sera from guinea-pig immunized with control OMVs is expected and attributed to the lower immunogenicity of fHbp in this species as suggested by the ELISA results.

[0236] In absence of zinc chelator, sera from mice immunized with ZnuD OMVs did not mediate the complement killing of the tested strains except four. These four strains are also killed by anti-fHbp antibodies. The role of these anti-fHbp antibodies was clearly demonstrated for the strain H44/76 by performing bactericidal assay with a .DELTA.fHbp H44/76 strain (Table 4). The same anti-ZnuD OMVs sera tested in a bactericidal assay allowing the expression of ZnuD allow to mediate the killing of 12 of the 14 strains tested. Similar results were observed with sera from guinea-pigs. In absence of TPEN in the culture media, strains were not killed in presence of active complement except three. Again, for one of these three strains, H44/76, the role of anti-fHbp antibodies was demonstrated by using a .DELTA.fHbp strain. In presence of TPEN, all the strains except two were killed with SBA titers clearly above the cut-off. The two strains that were not killed are the same strains not killed by the mouse sera. The expression of ZnuD by these two strains (760676 and M05-024072) was demonstrated by Western-blot analysis.

[0237] A third group of mice and guinea-pigs were immunized with OMVs purified from a H44/76 strain over-expressing an irrelevant antigen. In absence or presence of TPEN in the serum bactericidal assay, the results are similar to the results observed with the control sera (data not shown). In addition, bactericidal assay were also performed on one strain of each serogroup, A, C, W-135 and Y, confirming the cross-bactericidal activity of anti-ZnuD antibodies against these four serogroups (data not shown).

Confirmation of ZnuD and fHbp as Target of Bactericidal Antibodies

[0238] To confirm that ZnuD is a major target of bactericidal antibodies and because there was a trend to observe higher bactericidal titers in presence of TPEN, a delta znuD H44/76 strain was used in bactericidal assays with or without of TPEN (Table 4).

[0239] In absence of TPEN, the SBA titers were similar between the H44/76 WT and the delta znuD strains, whatever the origin of the sera (animal species and OMVs used for immunization). In this bactericidal condition, no killing was observed with the delta fHbp strain. These results demonstrate that fHbp is a major target of bactericidal antibodies in absence of ZnuD expression.

[0240] In presence of TPEN, the delta fHbp strain was not killed by sera from control animals while similar titers were measured for both the delta znuD strain and the WT H44/76 strain. As expected, when using anti-ZnuD OMVs sera, the bactericidal titers were lower for the delta znuD strain compared to H44/76.

[0241] These results demonstrate that ZnuD OMVs have induced both anti-fHbp and anti-ZnuD bactericidal antibodies, while the control OMVs have only elicited the production of anti-fHbp antibodies. The results also demonstrate that the co-expression of fHbp and ZnuD is not required to induce the complement killing mediated by bactericidal antibodies.

Discussion

[0242] ZnuD is not expressed on classical culture media such as Mueller Hinton medium or Tryptic Soy Broth medium. These media were developed for the culture of fastidious microorganisms and so are rich in oligo-elements. In MH agar, the total amount of zinc ranges from 200 .mu.g to 450 .mu.g/L depending on the manufacturer. In such media, free Zinc is probably available in such concentration that N. meningitidis does not require to specifically express an outer membrane receptor dedicated in zinc acquisition because passive diffusion of zinc via non-specific porins is probably sufficient to allow the growth of the bacterium. In bovine serum, the amount of free zinc is estimated to approximately 0.01 .mu.g/L (0.15 nM) corresponding to around 0.0008% of the total amount of zinc in the serum (800 .mu.g/L). In children, the total zinc concentration is estimated around 750 .mu.g/L and, by extrapolation, free zinc should be also around 0.01 .mu.g/L. In cerebrospinal fluids, the total concentration of zinc is around 111 .mu.g/L. The expression of ZnuD in human was indirectly demonstrated by the detection of antibodies in sera from convalescent patients. The peptide array results, also suggest an expression in carriers. Based on the intensity of the signal measured in the peptide array, it should be wrong to conclude that ZnuD expression in the upper respiratory tract is lower than that in the blood. Indeed, the experiment was designed to detect serum IgG which poorly reflects a mucosal immune response induced by micro-organism colonisation. Ongoing experiments aim to demonstrate the in-vivo expression of ZnuD by performing staining of N. meningitidis directly in the cerebrospinal fluids of acute patients.

[0243] To allow the expression of ZnuD on classical media, the use of zinc chelator is required. This does not set a precedent because the use of metal chelator was already described to demonstrate the potential of iron binding proteins such as TbpA/B. However, the impact of the concentration of TPEN on the level of ZnuD expression was investigated (data not shown) with the aim to select a concentration that should correspond to the in-vivo ZnuD expression level. For this purpose, the expression of ZnuD in a delta zur strain was used as benchmark. From dose-range experiments, the concentration of 20 .mu.M of TPEN was selected. It is to be noted that in the attempt to avoid the use of chelator, other media are under evaluation. Promising results were obtained with the Catlin medium which is a chemically defined medium developed previously for the growth of Neisseria. Flow cytometry analysis suggests that ZnuD expression in this medium is similar to the expression of ZnuD by a delta zur strain growing on MH agar (data not shown).

[0244] Using the culture condition selected to potentially mimic the in-vivo expression of ZnuD (MH agar+20 .mu.M TPEN), a panel of 43 strains was assessed for the expression of this OMP. ZnuD was detected in all these strains. Globally, 182 N. meningitidis strains have been analysed for their expression of ZnuD. ZnuD expression was demonstrated in all strains except 7 serogroup Y strains all recently isolated in Germany and from the ST-23 clonal complex. For 4 of them, absence of expression was linked to a stop mutation, for the remaining 3 strains, there is no explanation based on nucleotide sequence analysis. A more careful attention will be put to assess the expression of ZnuD in serogroup Y strains, because its expression was demonstrated in older MenY strains.

[0245] Because ZnuD is an integral OMP, we have selected OMVs for its presentation to the immune system. These OMVs were extracted from a .DELTA.galE and .DELTA.porA H44/76 strain by 0.5% DOC to reduce the LOS content. In such conditions, we have previously demonstrated that this kind of OMVs did not induce the production of bactericidal antibodies directed against the LOS. Mice and guinea-pigs immunized with control OMVs have induced a limited bactericidal response against few strains. This protective response is most probably related to the production of some anti-fHbp antibodies as demonstrated by their presence in ELISA and by the use of .DELTA.fHbp strain in bactericidal assay. The results also suggested that fHbp is a highly immunogenic protein, especially in mice, when presented in its natural conformation in OMVs even if present in low amount in these OMVs due to the use of detergent for the extraction of the vesicles. Both mice and guinea-pigs immunized with ZnuD OMVs have elicited the production of anti-ZnuD antibodies. Based on the ELISA data, the immunogenicity of ZnuD in the two species appears to be quite similar by contrast to fHbp. The anti-ZnuD OMVs sera were able to mediate the complement killing of all the strains tested whatever the serogroup but only in culture condition allowing the expression of ZnuD. The exception is two serogroup B strains for which absence of killing is not due to absence of ZnuD expression. Further investigations are ongoing to explain this absence of killing. Among the strains killed two are of interest because they have been used recently in an infant clinical trial assessing the cross-protection induced by a multivalent vaccine based on 5 proteins and OMVs derived from the NZ98/254 strain. Sera from subjects immunized with this complex vaccine failed to mediate the killing of the strain M01-240355 and only 50% of the infants have shown a cross-protection against the strain M01-240101 at the age of 1 year. These two strains were isolated in United Kingdom and are from ST-213 and ST-269 clonal complexes, respectively. These two clonal complexes represent 39% of MenB strains isolated from UK cases in 2008. In the present preclinical experiments, both mice and guinea-pigs immunized with ZnuD OMVs have elicited a high bactericidal response against these two strains (rabbit SBA titers>1700). For the strain M01-240355, this killing is not due to the co-presence of anti-ZnuD and anti-fHbp B antibodies because this strain expresses fHbp A. In addition, we have confirmed that the strain M01-240101 is also killed by anti-ZnuD antibodies in presence of human complement (data not shown).

[0246] In conclusion, ZnuD is a protein very well conserved, expressed by all serogroup B strains and able to mediate a cross-bactericidal response against most of the serogroup B strains tested and also against serogroup A, C, W and Y strains. An advantage of ZnuD is that ZnuD is very well conserved and up to now only one family is described. A more comprehensive vaccine could combine both fHbp and ZnuD.

Example 4

Calprotectin Binding to TdfH

[0247] Calprotectin is produced by neutrophils, macrophages and squamous epithelia. It is used as a diagnostic tool in faeces for Inflammatory bowel disease. Furthermore it is upregulated upon contact with LPS and it can be found in cerebrospinal fluid when the patient has meningitis. Calprotectin is a heterotetramer of human proteins S100A8 and S100A9 and can bind two zinc or manganese atoms. When calprotectin is spotted on a plate where Neisseria is plated in a confluent layer there is an initial inhibition of growth, due to the sequestering of zinc and/or manganese. We believe that Neisseria can take the zinc and/or manganese from only one of the binding sites (the low affinity site). The initial clearing comes from the sequestering of zinc and/or manganese to the high affinity site, causing growth inhibition. When also the low affinity site becomes loaded with zinc and/or manganese Neisseria can use these metals by taking up though TdfH. Therefore, after the initial clearing, growth is observed in the clearing zone. Furthermore, the uptake process needs energy from the proton motive force in the inner membrane that is shuttled to the outer membrane by TonB, as shown by the lack of growth of the TonB mutant in the clearing zone.

TABLE-US-00008 Strain Growth in clearing zone H44/76 cps- Yes TdfH K.O. No Tdfl K.O. Yes TdfH/Tdfl K.O. No TonB K.O. No

[0248] A pEN11 derivative that expressed TdfH in high amounts was used to test whether TdfH can bind to calprotectin. B2540 with pEN11-TdfH-terminator was grown in TSB medium with and without 1 mM IPTG. 1 OD.sub.550nm of bacteria were taken after 6 hours of growth and washed once in HBSS. The bacteria were resuspended in 1 ml HBSS and 4 ug of calprotectin was added. Calprotectin was allowed to bind for 1 hour after which the cells were washed twice in HBSS. The pellet was then resuspended in sample buffer and boiled for 10 minutes. The lysates where then analyzed by SDS-PAGE and western blots with anti-calprotectin (FIG. 15.)

[0249] As shown in FIG. 15, a signal was detectable but was weaker than expected for the amount of TdfH present in the samples. It was then investigated if the binding is better when zinc or manganese is added to the binding step (FIG. 16). Significantly more calprotectin was bound to TdfH when zinc or manganese was present.

Sequence CWU 1

1

1191758PRTNeisseria meningitidis MC58 1Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Glu Gln Ser Val Asp 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Val Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asn Ala His Ala His 305 310 315 320 Thr His Ser Gly Arg Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Ile Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Gln Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Phe His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 2695PRTAcidovorax sp JS42 2Met Asn Ala Phe Thr Pro Phe Ala Arg Met Pro Gly Ala Met His Pro 1 5 10 15 Leu Ala Trp Ala Ala Leu Leu Cys Leu Thr Ser Ala Ala Pro Ala Trp 20 25 30 Ala Gln Ala Asp Ala Arg Leu Pro Glu Val Thr Val Ser Ser Ser Gly 35 40 45 Leu Gln Leu Gly Val Ser Glu Met Thr Gln Pro Val Ser Val Leu Glu 50 55 60 Gly Asp Ala Leu Val Arg Gln Arg Glu Ala Thr Leu Gly Glu Thr Leu 65 70 75 80 Asp Gly Glu Pro Gly Ile Thr Gly Ser His Phe Gly Ala Gly Ala Ser 85 90 95 Arg Pro Val Ile Arg Gly Met Asp Gly Pro Arg Val Arg Val Leu Ser 100 105 110 Asp Gly Ser Glu Leu His Asp Ala Ser Thr Val Ser Pro Asp His Ala 115 120 125 Val Ala Ala Glu Pro Leu Leu Ala Thr Gln Val Glu Val Leu Arg Gly 130 135 140 Pro Ser Ala Leu Val Tyr Gly Gly Gly Ala Val Gly Gly Val Val Asn 145 150 155 160 Val Leu Asp Gly Lys Val Pro Thr Ala Val Pro Asp Lys Gly Tyr Glu 165 170 175 Gly Ser Ala Glu Leu Arg Ala Gly Ser Ala Ala Arg Glu Lys Ala Gly 180 185 190 Ala Val Ala Leu Thr Gly Gly Ala Gly Asn Leu Ala Val His Val Glu 195 200 205 Ala Ala Gly Arg Asp Ala Asp Asp Tyr Arg Val Gly Ser Gly Trp Ala 210 215 220 Glu Gly Arg Arg Val Pro Gly Ser Phe Asn Arg Thr Gly Thr Gly Ser 225 230 235 240 Val Gly Leu Ser Trp Val Gly Ser Arg Gly Tyr Leu Gly Leu Ala Phe 245 250 255 Thr Arg Gln Asn Ala Lys Tyr Gly Leu Pro Gly His Ser His Ser Phe 260 265 270 Glu Gly Cys His Thr His Gly Asn His Leu His Cys Gly Ser His Asp 275 280 285 Glu His Asp His Asp Asp Asp Gly His Asp His Asp His Asp His Gly 290 295 300 His Glu Ala Val Pro Val Val Asp Leu Arg Ser Glu Arg Val Asp Ile 305 310 315 320 Arg Gly Glu Leu Arg Asp Pro Phe Thr Gly Phe Ser Ala Leu Arg Leu 325 330 335 Arg Ala Gly Val Thr Asp Tyr Val His Asp Glu Val Glu Glu Gly Thr 340 345 350 Val Ala Thr Thr Phe Lys Asn Lys Ala His Asp Leu Arg Val Glu Leu 355 360 365 Gln His Glu Pro Val Ala Gly Trp Arg Gly Val Leu Gly Leu Gln Thr 370 375 380 Gly Gln Arg Lys Phe Ser Ala Ala Gly Glu Glu Ala Tyr Val Gln Pro 385 390 395 400 Thr Leu Thr Arg Gln Trp Gly Val Phe Leu Leu Glu Glu Tyr Arg Leu 405 410 415 Gly Asp Trp His Gly Asp Trp Arg Ile Glu Thr Ala Leu Arg His Asp 420 425 430 Arg Gln Ser Ala Glu Ala Gln Asp Ser Gly Val Glu Arg Arg His His 435 440 445 Gly Thr Ser Ala Ser Leu Gly Ala Val Trp Arg Phe Ala Pro Gly Tyr 450 455 460 Ala Ala Gly Ala Ser Ile Thr Arg Ala His Arg Ala Pro Thr Ala Glu 465 470 475 480 Glu Leu Tyr Ala Arg Gly Leu His Met Ala Thr Ser Thr Tyr Glu Arg 485 490 495 Gly Asp Ala Ser Leu Arg Ala Glu Thr Ser Arg Asn Ile Asp Leu Ser 500 505 510 Leu Arg Lys Thr Ser Gly Asp Thr Thr Phe Asp Val Ser Val Phe His 515 520 525 Asn Arg Ile Arg Asn Tyr Ile Tyr Gly Arg Thr Leu Asp Glu Leu Asp 530 535 540 Gly Leu Gln Leu Leu Gln Tyr Ser Gln Ala Gly Ala Thr Phe Thr Gly 545 550 555 560 Met Glu Gly Arg Val Arg Gln Arg Ile Thr Gln Arg Leu Gly Val Thr 565 570 575 Leu Phe Gly Asp Ser Val Arg Ala Arg Leu Asp Gly Gly Glu Arg Leu 580 585 590 Pro Arg Ile Ala Pro Ala Arg Val Gly Leu Arg Val Asp Ala Asn Trp 595 600 605 Arg Asp Trp Gly Gly Ala Val Glu Trp Val Gln Val Ala Arg Gln Asn 610 615 620 Arg Val Ala Ala Phe Glu Thr Ala Thr Pro Gly Tyr Gly Met Leu Asn 625 630 635 640 Leu Gly Leu Ala Tyr Asn Gly Arg Thr Gly Ser Gly Thr Pro Trp Gln 645 650 655 Val Tyr Leu Lys Ala Arg Asn Leu Thr Asp Arg Leu Ala Tyr Ala His 660 665 670 Thr Ser Phe Ile Lys Asp Ala Ala Pro Leu Ala Gly Arg Asn Val Thr 675 680 685 Val Gly Val His Val Ser Phe 690 695 3803PRTAcinetobacter baumannii 3Met Leu Asn Lys Ser Lys Leu Phe Leu Ala Leu Ile Thr Leu Gly Ala 1 5 10 15 Ser Lys Ile Val Leu Ala Ala Glu Gly Pro Val Thr Thr Leu Asn Thr 20 25 30 Ile Val Leu Thr Ala Gln Ser Asp Glu Leu Gly Ser Glu Leu Leu Gly 35 40 45 Lys Ser Leu Asn Val Ser Asn Gln Phe Ile Asp Thr Ser Lys Leu Lys 50 55 60 Gln Arg Ser Thr Thr Leu Gly Asp Ala Leu Gly Thr Glu Leu Gly Ile 65 70 75 80 His Ser Asn Gln Tyr Gly Gly Gly Ala Ser Thr Pro Ile Ile Arg Gly 85 90 95 Gln Glu Gly Lys Arg Ile Lys Val Leu Gln Asn Asn Ala Asp Val Leu 100 105 110 Asp Met Ser Asn Met Ser Pro Asp His Ala Val Thr Val Glu Pro Ser 115 120 125 Leu Ala Lys Ser Ile Glu Ile Ile Arg Gly Ala Ser Thr Leu Leu Tyr 130 135 140 Ser Ser Asn Ser Ala Ala Gly Val Val Asn Val Ile Asp Tyr Lys Ile 145 150 155 160 Pro Thr Gln Met Pro Gln Asp Gly Leu Glu Gly Asn Thr Thr Leu Arg 165 170 175 Phe Asn Thr Gly Ser Asn Glu Lys Leu Thr Thr Ala Gly Val Thr Val 180 185 190 Gly Leu Ser Pro His Val Ala Leu Arg Ala Glu Gly Leu Tyr Arg Asn 195 200 205 Ala Gly Asn Tyr Lys Thr Pro His Tyr Gln Ser Ser Ser Tyr Asn Ser 210 215 220 Leu Glu Asp Leu Glu Asn Gln Asn Ser Ile Tyr Lys Asn Leu Lys Tyr 225 230 235 240 Leu Pro Glu Ser Trp Ala Glu Ser Arg Val Gly Thr Leu Gly Leu Ser 245 250 255 Trp Ile Asp Asp Asn Thr Tyr Leu Gly Val Ser Tyr Thr His Arg His 260 265 270 Asp Glu Tyr Gly Leu Pro Ala His Ser His Leu Tyr Glu Gly Cys Gly 275 280 285 Ala Ser Ala Ile Gly Ile Asp Ser Arg Ile Ser Gly Leu Lys Asn Tyr 290 295 300 Leu Leu Tyr Tyr Pro Gln Leu Met Asp Glu Gln Asp Ile Asn Tyr Ile 305 310 315 320 Asn Pro Arg Pro Asp Cys His Gln His Asn His Ile His Glu Thr Asn 325 330 335 Phe Ser His Asn Ala Pro Tyr Ile Asp Leu Asn Thr Arg Arg Tyr Asp 340 345 350 Val Arg Gly Glu Phe Thr Gln Pro Phe Thr Gly Ile Asp Lys Ile Arg 355 360 365 Thr Ser Leu Ser Tyr Ile Asp Tyr Phe His Asn Glu Leu Glu Gly Asp 370 375 380 Lys Ile Thr Asn Phe Phe Lys Asn Thr Gly Lys Val Gly Arg Ile Glu 385 390 395 400 Leu Ser His Gln Pro Leu Gly Glu Leu Thr Gly Ile Leu Gly Leu Gln 405 410 415 Tyr Leu Glu Gln Asp Asn Ser Ala Leu Ser Pro Val His Ser Gln Glu 420 425 430 Gly His Thr Thr Tyr Leu Asp Asn Gln Gln Leu Leu Asn Arg Asn Val 435 440 445 Thr Lys Asn Phe Ser Val Phe Gly Leu Glu Lys Tyr Asn Trp Asn Asp 450 455 460 Phe Thr Phe Glu Leu Gly Ala Arg Ile Glu Lys Gln Lys Val Ser Met 465 470 475 480 Asp Tyr Asp Ile Glu Lys Ile Lys Asp Ser Met Lys Pro Trp Pro Asn 485 490 495 Lys Tyr Asn Ser Pro Tyr Val Glu Lys Asn Asn Lys Ile Arg Ala Gln 500 505 510 Asn Leu Lys Ser Ile Leu Glu Ala Val Gln Pro Asn Lys Glu Thr Ala 515 520 525 Phe Ser Tyr Ala Gly Thr Val His Trp Arg Phe Ala Pro Asn Tyr Ile 530 535 540 Leu Ser Leu Thr Gly Thr His Gln Glu Arg Leu Pro Asn Ala Gln Glu 545 550 555 560 Met Tyr Thr His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly 565 570 575 Asn Arg Phe Leu Arg Lys Glu Lys Ser Asn Asn Leu Glu Ile Ser Leu 580 585 590 Ala Tyr Lys Asp Asp Leu Leu Asp Tyr Gln Ile Ser Thr Tyr Tyr Tyr 595 600 605 Asp Phe Asp Asn Tyr Ile Tyr Leu Gln Thr Leu Asn Glu Val Leu Gly 610 615 620 Thr Thr Lys Val Arg Asp Gln His Thr Leu Arg Ile Asn His Tyr Ser 625 630 635 640 Gln Ser Ala Ala Asn Phe Tyr Gly Leu Glu Gly Asn Ile Gly Tyr Gln 645 650 655 Phe Asn Ser Val Tyr His Gly Ser Leu Phe Gly Asp Tyr Val Lys Gly 660 665 670 Arg Leu Thr Asn Leu Pro Asp Ala Val Ile Ala Tyr Asp Ile Trp Asn 675 680 685 Arg Glu Pro Thr Leu Ala Pro Gln Lys Asp Arg Tyr Thr Pro Arg Leu 690 695 700 Pro Pro Ala Arg Leu Gly Thr Arg Leu Lys Ala Asp Phe Asp Glu Ser 705 710 715 720 Leu Lys Gly Glu Ile Glu Tyr Tyr Arg Val Phe Lys Gln Asp Asn Ile 725 730 735 Ser Lys Phe Glu Gln Val Thr Ser Gly Tyr Asn Met Leu Asn Met Thr 740 745 750 Leu Ala Tyr Lys Asn Lys Leu Ser His Thr Glu Tyr Asp Leu Phe Phe 755 760 765 Lys Ala Asn Asn Leu Leu Asp Gln Lys Val Tyr Ala His Glu Thr Phe 770 775 780 Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Ser Leu Gly Leu Asn 785 790 795 800 Leu Asn Phe 4803PRTAcinetobacter baumannii 4Met Leu Asn Lys Ser Lys Leu Phe Leu Ala Leu Ile Thr Leu Gly Ala 1 5 10 15 Ser Lys Ile Leu Leu Ala Ala Glu Gly Pro Val Thr Ile Leu Asn Thr 20 25 30 Lys Val Leu Thr Ala

Gln Ser Asp Glu Leu Gly Ser Glu Leu Leu Gly 35 40 45 Lys Ser Leu Asn Val Ser Asn Gln Phe Ile Asp Thr Ser Lys Leu Lys 50 55 60 Gln Arg Ser Thr Thr Leu Gly Asp Ala Leu Gly Thr Glu Leu Gly Ile 65 70 75 80 His Ser Asn Gln Tyr Gly Gly Gly Ala Ser Thr Pro Ile Ile Arg Gly 85 90 95 Gln Glu Gly Lys Arg Ile Lys Val Leu Gln Asn Asn Ala Asp Val Leu 100 105 110 Asp Met Ser Asn Met Ser Pro Asp His Ala Val Thr Val Glu Pro Ser 115 120 125 Leu Ala Lys Ser Ile Glu Ile Ile Arg Gly Ala Ser Thr Leu Leu Tyr 130 135 140 Ser Ser Asn Ser Ala Ala Gly Val Val Asn Val Ile Asp Tyr Lys Ile 145 150 155 160 Pro Thr Gln Met Pro Gln Asp Gly Leu Glu Gly Asn Thr Thr Leu Arg 165 170 175 Phe Asn Thr Gly Ser Asn Glu Lys Leu Thr Thr Ala Gly Val Thr Val 180 185 190 Gly Leu Ser Pro His Val Ala Leu Arg Ala Glu Gly Leu Tyr Arg Asn 195 200 205 Ala Gly Asn Tyr Lys Thr Pro His Tyr Gln Ser Ser Ser Tyr Asn Ser 210 215 220 Leu Glu Asp Leu Glu Asn Gln Asn Ser Ile Tyr Lys Asn Leu Lys Tyr 225 230 235 240 Leu Pro Glu Ser Trp Ala Glu Ser Arg Val Gly Thr Leu Gly Leu Ser 245 250 255 Trp Ile Asp Asp Asn Thr Tyr Leu Gly Val Ser Tyr Thr His Arg His 260 265 270 Asp Glu Tyr Gly Leu Pro Ala His Ser His Leu Tyr Glu Gly Cys Gly 275 280 285 Ala Ser Ala Ile Gly Ile Asp Ser Arg Ile Ser Gly Leu Lys Asn Tyr 290 295 300 Leu Leu Tyr Tyr Pro Gln Leu Met Asp Glu Gln Asp Ile Asn Tyr Ile 305 310 315 320 Asn Pro Arg Pro Asp Cys His Gln His Asn His Ile His Glu Thr Asn 325 330 335 Phe Ser His Asn Ala Pro Tyr Ile Asp Leu Asn Thr Arg Arg Tyr Asp 340 345 350 Val Arg Gly Glu Phe Thr Gln Pro Phe Thr Gly Ile Asp Lys Ile Arg 355 360 365 Thr Ser Leu Ser Tyr Ile Asp Tyr Phe His Asn Glu Leu Glu Gly Asp 370 375 380 Lys Ile Thr Asn Phe Phe Lys Asn Thr Gly Lys Val Gly Arg Ile Glu 385 390 395 400 Leu Ser His Gln Pro Leu Gly Glu Leu Thr Gly Ile Leu Gly Leu Gln 405 410 415 Tyr Leu Glu Gln Asp Asn Ser Ala Leu Ser Pro Val His Ser Gln Glu 420 425 430 Gly His Thr Thr Tyr Leu Asp Asn Gln Gln Leu Leu Asn Arg Asn Val 435 440 445 Thr Lys Asn Phe Ser Val Phe Gly Leu Glu Lys Tyr Asn Trp Asn Asp 450 455 460 Phe Thr Phe Glu Leu Gly Ala Arg Ile Glu Lys Gln Lys Val Ser Met 465 470 475 480 Asp Tyr Asp Ile Glu Lys Ile Lys Asp Ser Met Lys Pro Trp Pro Asn 485 490 495 Lys Tyr Asn Ser Pro Tyr Val Glu Lys Asn Asn Lys Ile Arg Ala Gln 500 505 510 Asn Leu Lys Ser Ile Leu Glu Ala Val Gln Pro Asn Lys Glu Thr Ala 515 520 525 Phe Ser Tyr Ala Gly Thr Val His Trp Arg Phe Ala Pro Asn Tyr Ile 530 535 540 Leu Ser Leu Thr Gly Thr His Gln Glu Arg Leu Pro Asn Ala Gln Glu 545 550 555 560 Met Tyr Thr His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly 565 570 575 Asn Arg Phe Leu Arg Lys Glu Lys Ser Asn Asn Leu Glu Ile Ser Leu 580 585 590 Ala Tyr Lys Asp Asp Leu Leu Asp Tyr Gln Ile Ser Thr Tyr Tyr Tyr 595 600 605 Asp Phe Asp Asn Tyr Ile Tyr Leu Gln Thr Leu Asn Glu Val Leu Gly 610 615 620 Thr Thr Lys Val Arg Asp Gln His Thr Leu Arg Ile Asn His Tyr Ser 625 630 635 640 Gln Ser Ala Ala Asn Phe Tyr Gly Leu Glu Gly Asn Ile Gly Tyr Gln 645 650 655 Phe Asn Ser Val Tyr His Gly Ser Leu Phe Gly Asp Tyr Val Lys Gly 660 665 670 Arg Leu Thr Asn Leu Pro Asp Ala Val Ile Ala Tyr Asp Ile Trp Asn 675 680 685 Arg Glu Pro Thr Leu Ala Pro Gln Lys Asp Arg Tyr Thr Pro Arg Leu 690 695 700 Pro Pro Ala Arg Leu Gly Thr Arg Leu Lys Ala Asp Phe Asp Glu Ser 705 710 715 720 Leu Lys Gly Glu Ile Glu Tyr Tyr Arg Val Phe Lys Gln Asp Asn Ile 725 730 735 Ser Lys Phe Glu Gln Val Thr Ser Gly Tyr Asn Met Leu Asn Met Thr 740 745 750 Leu Ala Tyr Lys Asn Lys Leu Ser His Thr Glu Tyr Asp Leu Phe Phe 755 760 765 Lys Ala Asn Asn Leu Leu Asp Gln Lys Val Tyr Ala His Glu Thr Phe 770 775 780 Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Ser Leu Gly Leu Asn 785 790 795 800 Leu Asn Phe 5803PRTAcinetobacter baumannii 5Met Leu Asn Lys Ser Lys Leu Phe Leu Ala Leu Ile Thr Leu Gly Ala 1 5 10 15 Ser Lys Ile Leu Leu Ala Ala Glu Gly Pro Val Thr Thr Leu Asn Thr 20 25 30 Ile Val Leu Thr Ala Gln Ser Asp Glu Leu Gly Ser Glu Leu Leu Gly 35 40 45 Lys Ser Leu Asn Val Ser Asn Gln Phe Ile Asp Thr Ser Lys Leu Lys 50 55 60 Gln Arg Ser Thr Thr Leu Gly Asp Ala Leu Gly Thr Glu Leu Gly Ile 65 70 75 80 His Ser Asn Gln Tyr Gly Gly Gly Ala Ser Thr Pro Ile Ile Arg Gly 85 90 95 Gln Glu Gly Lys Arg Ile Lys Val Leu Gln Asn Asn Ala Asp Val Leu 100 105 110 Asp Met Ser Asn Met Ser Pro Asp His Ala Val Thr Val Glu Pro Ser 115 120 125 Leu Ala Lys Ser Ile Glu Ile Ile Arg Gly Ala Ser Thr Leu Leu Tyr 130 135 140 Ser Ser Asn Ser Ala Ala Gly Val Val Asn Val Ile Asp Tyr Lys Ile 145 150 155 160 Pro Thr Gln Met Pro Gln Asp Gly Leu Glu Gly Asn Thr Thr Leu Arg 165 170 175 Phe Asn Thr Gly Ser Asn Glu Lys Leu Thr Thr Ala Gly Val Thr Val 180 185 190 Gly Leu Ser Pro His Val Ala Leu Arg Ala Glu Gly Leu Tyr Arg Asn 195 200 205 Ala Gly Asn Tyr Lys Thr Pro His Tyr Gln Ser Ser Ser Tyr Asn Ser 210 215 220 Leu Glu Asp Leu Glu Asn Gln Asn Ser Ile Tyr Lys Asn Leu Lys Tyr 225 230 235 240 Leu Pro Glu Ser Trp Ala Glu Ser Arg Val Gly Thr Leu Gly Leu Ser 245 250 255 Trp Ile Asp Asp Asn Thr Tyr Leu Gly Val Ser Tyr Thr His Arg His 260 265 270 Asp Glu Tyr Gly Leu Pro Ala His Ser His Leu Tyr Glu Gly Cys Gly 275 280 285 Ala Ser Ala Ile Gly Ile Asp Ser Arg Ile Ser Gly Leu Lys Asn Tyr 290 295 300 Leu Leu Tyr Tyr Pro Gln Leu Met Asp Glu Gln Asp Ile Asn Tyr Ile 305 310 315 320 Asn Pro Arg Pro Asp Cys His Gln His Asn His Ile His Glu Thr Asn 325 330 335 Phe Ser His Asn Ala Pro Tyr Ile Asp Leu Asn Thr Arg Arg Tyr Asp 340 345 350 Val Arg Gly Glu Phe Thr Gln Pro Phe Thr Gly Ile Asp Lys Ile Arg 355 360 365 Thr Ser Leu Ser Tyr Ile Asp Tyr Phe His Asn Glu Leu Glu Gly Asp 370 375 380 Lys Ile Thr Asn Phe Phe Lys Asn Thr Gly Lys Val Gly Arg Ile Glu 385 390 395 400 Leu Ser His Gln Pro Leu Gly Glu Leu Thr Gly Ile Leu Gly Leu Gln 405 410 415 Tyr Leu Glu Gln Asp Asn Ser Ala Leu Ser Pro Val His Ser Gln Glu 420 425 430 Gly His Thr Thr Tyr Leu Asp Asn Gln Gln Leu Leu Asn Arg Asn Val 435 440 445 Thr Lys Asn Phe Ser Val Phe Gly Leu Glu Lys Tyr Asn Trp Asn Asp 450 455 460 Phe Thr Phe Glu Leu Gly Ala Arg Ile Glu Lys Gln Lys Val Ser Met 465 470 475 480 Asp Tyr Asp Ile Glu Lys Ile Lys Asp Ser Met Lys Pro Trp Pro Asn 485 490 495 Lys Tyr Asn Ser Pro Tyr Val Glu Lys Asn Asn Lys Ile Arg Ala Gln 500 505 510 Asn Leu Lys Ser Ile Leu Glu Ala Val Gln Pro Asn Lys Glu Thr Ala 515 520 525 Phe Ser Tyr Ala Gly Thr Val His Trp Arg Phe Ala Pro Asn Tyr Ile 530 535 540 Leu Ser Leu Thr Gly Thr His Gln Glu Arg Leu Pro Asn Ala Gln Glu 545 550 555 560 Met Tyr Thr His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly 565 570 575 Asn Arg Phe Leu Arg Lys Glu Lys Ser Asn Asn Leu Glu Ile Ser Leu 580 585 590 Ala Tyr Lys Asp Asp Leu Leu Asp Tyr Gln Ile Ser Thr Tyr Tyr Tyr 595 600 605 Asp Phe Asp Asn Tyr Ile Tyr Leu Gln Thr Leu Asn Glu Val Leu Gly 610 615 620 Thr Thr Lys Val Arg Asp Gln His Thr Leu Arg Ile Asn His Tyr Ser 625 630 635 640 Gln Ser Ala Ala Asn Phe Tyr Gly Leu Glu Gly Asn Ile Gly Tyr Gln 645 650 655 Phe Asn Ser Val Tyr His Gly Ser Leu Phe Gly Asp Tyr Val Lys Gly 660 665 670 Arg Leu Thr Asn Leu Pro Asp Ala Val Ile Ala Tyr Asp Ile Trp Asn 675 680 685 Arg Glu Pro Thr Leu Ala Pro Gln Lys Asp Arg Tyr Thr Pro Arg Leu 690 695 700 Pro Pro Ala Arg Leu Gly Thr Arg Leu Lys Ala Asp Phe Asp Glu Ser 705 710 715 720 Leu Lys Gly Glu Ile Glu Tyr Tyr Arg Val Phe Lys Gln Asp Asn Ile 725 730 735 Ser Lys Phe Glu Gln Val Thr Ser Gly Tyr Asn Met Leu Asn Met Thr 740 745 750 Leu Ala Tyr Lys Asn Lys Leu Ser His Thr Glu Tyr Asp Leu Phe Phe 755 760 765 Lys Ala Asn Asn Leu Leu Asp Gln Lys Val Tyr Ala His Glu Thr Phe 770 775 780 Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Ser Leu Gly Leu Asn 785 790 795 800 Leu Asn Phe 6685PRTAcinetobacter baumannii AB900 6Met Leu Phe Tyr Lys Asn Ile Leu Thr Leu Ser Ile Leu Ala Ala Ile 1 5 10 15 Ser Ile Pro Val Phe Ala Ala Glu Asn Glu Asn Val Glu Lys Leu Glu 20 25 30 Thr Ile Arg Ile Lys Ala His Pro Leu Glu Gln Thr Ser Lys Asp Phe 35 40 45 Ala Val Ala Asp Thr Val Val Asp Gln Lys His Leu Thr Glu Gly Ala 50 55 60 Ala Thr Ile Gly Asp Ala Leu Asn Ser Glu Val Gly Ile Tyr Ala Asn 65 70 75 80 Gln Phe Gly Ala Gly Ser Ser Arg Pro Val Ile Arg Gly Gln Asp Gly 85 90 95 Pro Arg Val Lys Val Leu Gln Asn Ser Ser Glu Asn Val Asp Val Ser 100 105 110 Thr Leu Ser Pro Asp His Ala Val Thr Val Asp Pro Val Leu Ala Lys 115 120 125 Gln Val Glu Val Ile Arg Gly Pro Ser Thr Leu Leu Phe Gly Ala Gly 130 135 140 Thr Ile Gly Gly Leu Val Asn Val Ile Asp Asn Lys Ile Pro Thr Gln 145 150 155 160 Met Pro Glu Asn Gly Tyr Glu Gly Gln Val Gly Leu Arg Tyr Asn Thr 165 170 175 Gly Ser Asp Glu Lys Leu Ala Ser Ala Gly Val Thr Val Gly Leu Gly 180 185 190 Ser Gln Val Ala Leu Arg Val Glu Gly Leu Thr Arg Asp Ala Asn Asn 195 200 205 Tyr Ile Ala Pro Asn Tyr Ile His Glu Gly Glu Lys Glu Arg Arg Val 210 215 220 Asp Asn Thr Phe Ala Gln Gly Asp Ser Val Asn Val Gly Leu Ser Trp 225 230 235 240 Ile Tyr Asp Arg Gly Tyr Thr Gly Ile Ser Tyr Ser Asn Arg Arg Asp 245 250 255 Gln Tyr Gly Leu Pro Gly His Ser His Glu Tyr Glu Thr Cys His Ile 260 265 270 His Asp Leu Ser Leu His Cys Gly Asp His Asp His Glu Gly His Ser 275 280 285 Asp Glu Glu Ala His Asp His Glu His Glu His Gly Gly Pro Trp Ile 290 295 300 Asp Leu Lys Ser Glu Arg Tyr Asp Phe Lys Thr Glu Leu Asn Asp Pro 305 310 315 320 Phe Ala Gly Phe Gln Lys Leu Arg Ala Gln Ala Ser Tyr Thr Asp Tyr 325 330 335 Gln His Asp Glu Ile Glu Glu Gly Ala Ile Ala Thr Arg Phe Gln Asn 340 345 350 Lys Gly Tyr Asp Gly Arg Ile Glu Leu Val His Thr Pro Ile Ala Asp 355 360 365 Trp Glu Gly Val Ile Gly Thr Gln Leu Gly Gln Gln Lys Leu Asn Leu 370 375 380 Thr Gly Glu Glu Ala Phe Met Ala Pro Thr Thr Thr Lys Lys Trp Ser 385 390 395 400 Val Phe Ala Leu Glu His Lys Gln Trp Lys Asp Val His Phe Glu Leu 405 410 415 Ser Ala Arg Ala Asp Gln Gln Glu Ile Asp Val Asp Asp Asn Ser Lys 420 425 430 Gln Asp Phe Asp Gly Ser Ala Phe Ser Tyr Ala Gly Ala Ala Asn Trp 435 440 445 Glu Phe Ala Pro Asn Tyr Lys Leu Ser Phe Val Ala Ser His Gln Glu 450 455 460 Arg Leu Pro Leu Ala Gln Glu Leu Tyr Ala Asn Gly Ala His Phe Ala 465 470 475 480 Thr Asn Thr Tyr Glu Leu Gly Asn Asp Gln Leu Ser Lys Glu Lys Ser 485 490 495 Asn Asn Val Glu Leu Gly Leu His Phe Asp Asn Asp Lys Leu Asp Tyr 500 505 510 His Leu His Val Tyr His Asn Trp Phe Asp Asp Tyr Ile Tyr Ala Gln 515 520 525 Thr Leu Asp Arg Tyr Lys Asp Phe Arg Leu Val Gln Tyr Thr Gln Asp 530 535 540 Lys Ala Arg Phe Tyr Gly Ala Glu Gly Glu Ile Gly Tyr Gln Ile Thr 545 550 555 560 Pro Met Tyr Lys Ile Ser Ala Phe Gly Asp Tyr Val Arg Gly Lys Ile 565 570 575 Asp Ala Glu Gly Asn Ala Pro Arg Ile Pro Ala Gly Arg Leu Gly Thr 580 585 590 Lys Val Asp Ala Asp Phe Gly Asp Gly Phe Ser Gly Ser Ala Glu Tyr 595 600 605 Tyr His Val Phe Asn Gln Asp Lys Ile Ala Ala Tyr Glu Thr Glu Thr 610 615 620 Glu Gly Tyr Asn Met Leu Asn Leu Gly Val Ala Tyr Ser Gly Gln Tyr 625 630 635 640 Gly Ala Lys Thr Asp Tyr Arg Val Tyr Leu Lys Ala Asn Asn Leu Leu 645 650 655 Asp Asp Thr Val Tyr Gln His Ala Ser Phe Leu Ser Asn Ile Pro Gln 660 665 670 Val Gly Arg Asn Phe Thr Val Gly Val Asp Phe Ser Phe 675 680 685 7685PRTAcinetobacter baumannii ACICU 7Met Leu Phe Tyr Lys Asn Ile Leu Thr Leu Ser Ile Leu Ala Ala Ile 1 5 10 15 Ser Ile Pro Val Phe Ala Ala Glu Asn Glu Asn Val Glu Lys Leu Glu 20 25 30 Thr Ile Arg Ile Lys Ala His Pro Leu Glu Gln Thr Ser Lys

Asp Phe 35 40 45 Ala Val Ala Asp Thr Val Val Asp Gln Lys His Leu Thr Glu Gly Ala 50 55 60 Ala Thr Ile Gly Asp Ala Leu Asn Ser Glu Val Gly Ile Tyr Ala Asn 65 70 75 80 Gln Phe Gly Ala Gly Ser Ser Arg Pro Val Ile Arg Gly Gln Asp Gly 85 90 95 Pro Arg Val Lys Val Leu Gln Asn Ser Ser Glu Asn Val Asp Val Ser 100 105 110 Thr Leu Ser Pro Asp His Ala Val Thr Val Asp Pro Val Leu Ala Lys 115 120 125 Gln Val Glu Val Ile Arg Gly Pro Ser Thr Leu Leu Phe Gly Ala Gly 130 135 140 Thr Val Gly Gly Leu Val Asn Val Ile Asp Asn Lys Ile Pro Thr Gln 145 150 155 160 Met Pro Glu Asn Gly Tyr Glu Gly Gln Val Gly Leu Arg Tyr Asn Thr 165 170 175 Gly Ser Asp Glu Lys Leu Ala Ser Ala Gly Val Thr Val Gly Leu Gly 180 185 190 Ser Gln Val Ala Leu Arg Val Glu Gly Leu Thr Arg Asp Ala Asn Asn 195 200 205 Tyr Ile Ala Pro Asn Tyr Ile His Glu Gly Glu Lys Glu Arg Arg Val 210 215 220 Asp Asn Thr Phe Ala Gln Gly Asp Ser Val Asn Val Gly Leu Ser Trp 225 230 235 240 Ile Tyr Asp Arg Gly Tyr Thr Gly Ile Ser Tyr Ser Asn Arg Arg Asp 245 250 255 Gln Tyr Gly Leu Pro Gly His Ser His Glu Tyr Glu Thr Cys His Ile 260 265 270 His Asp Leu Ser Leu His Cys Gly Asp His Asp His Glu Gly His Ser 275 280 285 Asp Glu Glu Ala His Asp His Glu His Glu His Gly Gly Pro Trp Ile 290 295 300 Asp Leu Lys Ser Glu Arg Tyr Asp Phe Lys Thr Glu Leu Asn Asp Pro 305 310 315 320 Phe Ala Gly Phe Gln Lys Leu Arg Ala Gln Ala Ser Tyr Thr Asp Tyr 325 330 335 Gln His Asp Glu Ile Glu Glu Gly Thr Ile Ala Thr Arg Phe Gln Asn 340 345 350 Lys Gly Tyr Asp Gly Arg Ile Glu Leu Val His Asn Pro Ile Ala Asp 355 360 365 Trp Glu Gly Val Ile Gly Thr Gln Leu Gly Gln Gln Lys Leu Asn Leu 370 375 380 Thr Gly Glu Glu Ala Phe Met Ala Pro Thr Thr Thr Lys Lys Trp Ser 385 390 395 400 Val Phe Ala Leu Glu His Lys Gln Trp Lys Asp Val His Phe Glu Leu 405 410 415 Ser Ala Arg Ala Asp Gln Gln Glu Ile Asp Val Asp Asp Asn Ser Lys 420 425 430 Gln Asp Phe Asp Gly Ser Ala Phe Ser Tyr Ala Gly Ala Ala Asn Trp 435 440 445 Glu Phe Ala Pro Asn Tyr Lys Leu Ser Phe Val Ala Ser His Gln Glu 450 455 460 Arg Leu Pro Leu Ala Gln Glu Leu Tyr Ala Asn Gly Ala His Phe Ala 465 470 475 480 Thr Asn Thr Tyr Glu Leu Gly Asn Asp Gln Leu Gly Lys Glu Lys Ser 485 490 495 Asn Asn Val Glu Leu Gly Leu His Phe Asp His Asp Lys Leu Asp Tyr 500 505 510 His Leu His Val Tyr His Asn Trp Phe Asp Asp Tyr Ile Tyr Ala Gln 515 520 525 Thr Leu Asp Arg Tyr Lys Asp Phe Arg Leu Val Gln Tyr Thr Gln Asp 530 535 540 Lys Ala Arg Phe Tyr Gly Ala Glu Gly Glu Ile Gly Tyr Gln Ile Thr 545 550 555 560 Pro Ile Tyr Lys Ile Ser Ala Phe Gly Asp Tyr Val Arg Gly Lys Ile 565 570 575 Asp Ala Glu Gly Asn Ala Pro Arg Ile Pro Ala Gly Arg Leu Gly Thr 580 585 590 Lys Val Asp Ala Asp Phe Gly Asp Gly Phe Ser Gly Ser Ala Glu Tyr 595 600 605 Tyr His Val Phe Asn Gln Asp Lys Ile Ala Ala Tyr Glu Thr Glu Thr 610 615 620 Glu Gly Tyr Asn Met Leu Asn Leu Gly Val Ala Tyr Ser Gly Gln Tyr 625 630 635 640 Gly Ala Lys Thr Asp Tyr Arg Val Tyr Leu Lys Ala Asn Asn Leu Leu 645 650 655 Asp Asp Thr Val Tyr Gln His Ala Ser Phe Leu Ser Asn Ile Pro Gln 660 665 670 Val Gly Arg Asn Phe Thr Val Gly Val Asp Phe Ser Phe 675 680 685 8803PRTAcinetobacter baumannii ATCC 17978 8Met Leu Asn Lys Ser Lys Leu Phe Leu Ala Leu Ile Thr Leu Gly Ala 1 5 10 15 Ser Lys Ile Leu Leu Ala Ala Glu Gly Pro Val Thr Thr Leu Asn Thr 20 25 30 Ile Val Leu Thr Ala Gln Ser Asp Glu Leu Gly Ser Glu Leu Leu Gly 35 40 45 Lys Ser Leu Asn Val Ser Asn Gln Phe Ile Asp Thr Ser Lys Leu Lys 50 55 60 Gln Arg Ser Thr Thr Leu Gly Asp Ala Leu Gly Thr Glu Leu Gly Ile 65 70 75 80 His Ser Asn Gln Tyr Gly Gly Gly Ala Ser Ala Pro Ile Ile Arg Gly 85 90 95 Gln Glu Gly Lys Arg Ile Lys Val Leu Gln Asn Asn Ala Asp Val Leu 100 105 110 Asp Met Ser Asn Met Ser Pro Asp His Ala Val Thr Val Glu Pro Ser 115 120 125 Leu Ala Lys Ser Ile Glu Ile Ile Arg Gly Ala Ser Thr Leu Leu Tyr 130 135 140 Ser Ser Asn Ser Ala Ala Gly Val Val Asn Val Ile Asp Tyr Lys Ile 145 150 155 160 Pro Thr Gln Met Pro Gln Asp Gly Leu Glu Gly Asn Thr Thr Leu Arg 165 170 175 Phe Asn Thr Gly Ser Asn Glu Lys Leu Thr Thr Ala Gly Val Thr Val 180 185 190 Gly Leu Ser Pro Arg Val Ala Leu Arg Ala Glu Gly Leu Tyr Arg Asn 195 200 205 Ala Gly Asn Tyr Lys Thr Pro His Tyr Gln Ser Ser Ser Tyr Asn Ser 210 215 220 Leu Glu Asp Leu Glu Asn Gln Asn Ile Val Tyr Lys Asn Leu Lys Tyr 225 230 235 240 Leu Pro Glu Ser Trp Ala Glu Ser Arg Leu Gly Thr Leu Gly Leu Ser 245 250 255 Trp Ile Asp Asp Asn Thr Tyr Leu Gly Val Ser Tyr Thr His Arg His 260 265 270 Asp Glu Tyr Gly Leu Pro Ala His Ser His Leu Tyr Glu Gly Cys Gly 275 280 285 Ala Ser Ala Ile Ser Ile Asn Thr Arg Ile Ser Gly Leu Lys Asn Tyr 290 295 300 Leu Leu Tyr Tyr Pro Gln Leu Met Glu Glu Gln Asp Ile Asn Tyr Val 305 310 315 320 Asn Pro Arg Pro Asp Cys His Gln His Asn His Ile His Glu Thr Thr 325 330 335 Phe Ser His Asn Ala Pro Tyr Ile Asp Leu Asn Thr Arg Arg Tyr Asp 340 345 350 Met Arg Gly Glu Phe Thr Gln Pro Phe Thr Gly Ile Asp Lys Ile Arg 355 360 365 Thr Ser Leu Ser Tyr Ile Asp Tyr Phe His Asn Glu Leu Glu Gly Asp 370 375 380 Lys Ile Thr Asn Phe Phe Lys Asn Thr Gly Lys Val Gly Arg Ile Glu 385 390 395 400 Leu Ser His Gln Pro Leu Gly Glu Leu Thr Gly Ile Leu Gly Leu Gln 405 410 415 Tyr Leu Glu Gln Asp Asn Ser Ala Leu Ser Pro Val His Ser Gln Glu 420 425 430 Gly His Thr Thr Tyr Leu Asp Thr Gln Gln Leu Leu Asn Arg Asn Val 435 440 445 Thr Lys Asn Phe Ser Val Phe Gly Leu Glu Lys Tyr Asn Trp Asn Asp 450 455 460 Phe Thr Phe Glu Leu Gly Ala Arg Ile Glu Lys Gln Lys Val Ser Met 465 470 475 480 Asp Tyr Asp Ile Glu Lys Ile Lys Asp Ser Met Lys Pro Trp Pro Asn 485 490 495 Lys Tyr Asn Ser Pro Tyr Val Glu Lys Asn Asn Lys Ile Arg Ala Gln 500 505 510 Asn Leu Lys Ser Ile Leu Glu Ala Val Gln Pro Asn Lys Glu Thr Ala 515 520 525 Phe Ser Tyr Ala Gly Thr Val His Trp Arg Phe Ala Pro Asn Tyr Ile 530 535 540 Leu Ser Leu Thr Gly Thr His Gln Glu Arg Leu Pro Asn Ala Gln Glu 545 550 555 560 Met Tyr Thr His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly 565 570 575 Asn Arg Phe Leu Arg Lys Glu Lys Ser Asn Asn Leu Glu Ile Ser Leu 580 585 590 Ala Tyr Lys Asp Asp Leu Leu Asp Tyr Gln Ile Ser Thr Tyr Tyr Tyr 595 600 605 Asp Phe Asp Asn Tyr Ile Tyr Leu Gln Thr Leu Asn Glu Val Leu Gly 610 615 620 Thr Thr Lys Val Arg Asp Gln His Thr Leu Arg Ile Asn His Tyr Ser 625 630 635 640 Gln Ser Ala Ala Asn Phe Tyr Gly Leu Glu Gly Asn Ile Gly Tyr Gln 645 650 655 Phe Asn Ser Val Tyr His Gly Ser Leu Phe Gly Asp Tyr Val Lys Gly 660 665 670 Arg Leu Thr Asn Leu Pro Asp Ala Val Ile Ala Tyr Asp Ile Trp Asn 675 680 685 Arg Glu Pro Thr Leu Ala Pro Gln Lys Asp Arg Tyr Thr Pro Arg Leu 690 695 700 Pro Pro Ala Arg Leu Gly Thr Arg Leu Lys Ala Asp Phe Asp Glu Ser 705 710 715 720 Leu Lys Gly Glu Ile Glu Tyr Tyr Arg Val Phe Lys Gln Asp Asn Ile 725 730 735 Ser Lys Phe Glu Gln Val Thr Ser Gly Tyr Asn Met Leu Asn Met Thr 740 745 750 Leu Ala Tyr Lys Asn Lys Leu Ser His Thr Glu Tyr Asp Leu Phe Phe 755 760 765 Lys Ala Asn Asn Leu Leu Asp Gln Lys Val Tyr Ala His Glu Thr Phe 770 775 780 Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Ser Leu Gly Leu Asn 785 790 795 800 Leu Asn Phe 9685PRTAcinetobacter baumannii SDF 9Met Leu Phe Tyr Lys Asn Ile Leu Thr Leu Ser Ile Leu Ala Ala Ile 1 5 10 15 Ser Ile Pro Val Phe Ala Ala Glu Asn Glu Asn Val Glu Lys Leu Glu 20 25 30 Thr Ile Arg Ile Lys Ala His Pro Leu Glu Gln Thr Ser Lys Asp Phe 35 40 45 Ala Val Ala Asp Thr Val Val Asp Gln Lys His Leu Thr Glu Gly Ala 50 55 60 Ala Thr Ile Gly Asp Ala Leu Asn Ser Glu Val Gly Ile Tyr Ala Asn 65 70 75 80 Gln Phe Gly Ala Gly Ser Ser Arg Pro Val Ile Arg Gly Gln Asp Gly 85 90 95 Pro Arg Val Lys Val Leu Gln Asn Ser Ser Glu Asn Val Asp Val Ser 100 105 110 Thr Leu Ser Pro Asp His Ala Val Thr Val Asp Pro Val Leu Ala Lys 115 120 125 Gln Val Glu Val Ile Arg Gly Pro Ser Thr Leu Leu Phe Gly Ala Gly 130 135 140 Thr Val Gly Gly Leu Val Asn Val Ile Asp Asn Lys Ile Pro Thr Gln 145 150 155 160 Ile Pro Glu Asn Gly Tyr Glu Gly Gln Val Gly Leu Arg Tyr Asn Thr 165 170 175 Gly Ser Asp Glu Lys Leu Ala Ser Ala Gly Val Thr Val Gly Leu Gly 180 185 190 Ser Gln Val Ala Leu Arg Ile Glu Gly Leu Thr Arg Asp Ala Asn Asn 195 200 205 Tyr Ile Ala Pro Asn Tyr Ile His Glu Gly Glu Lys Glu Arg Arg Val 210 215 220 Asp Asn Thr Phe Ala Gln Gly Asp Ser Val Asn Val Gly Leu Ser Trp 225 230 235 240 Val Tyr Asp Arg Gly Tyr Thr Gly Ile Ser Tyr Ser Asn Arg Arg Asp 245 250 255 Gln Tyr Gly Leu Pro Gly His Ser His Glu Tyr Glu Thr Cys His Ile 260 265 270 His Asp Leu Ser Leu His Cys Gly Asp His Asp His Glu Gly His Ser 275 280 285 Asp Glu Glu Ala His Asp His Glu His Glu His Gly Gly Pro Trp Ile 290 295 300 Asp Leu Lys Ser Glu Arg Tyr Asp Phe Lys Thr Glu Leu Asn Asp Pro 305 310 315 320 Phe Ala Gly Phe Gln Lys Leu Arg Ala Gln Ala Ser Tyr Thr Asp Tyr 325 330 335 Gln His Asp Glu Ile Glu Glu Gly Thr Ile Ala Thr Arg Phe Gln Asn 340 345 350 Lys Gly Tyr Asp Gly Arg Ile Glu Leu Val His Asn Pro Ile Ala Asp 355 360 365 Trp Glu Gly Val Ile Gly Thr Gln Leu Gly Gln Gln Lys Leu Asn Leu 370 375 380 Thr Gly Glu Glu Ala Phe Met Ala Pro Thr Thr Thr Lys Lys Trp Ser 385 390 395 400 Val Phe Ala Leu Glu His Lys Gln Trp Lys Asp Val His Phe Glu Leu 405 410 415 Ser Ala Arg Ala Asp Gln Gln Glu Ile Asp Val Asp Asp Asn Ser Lys 420 425 430 Gln Asp Phe Asp Gly Ser Ala Phe Ser Tyr Ala Gly Ala Ala Asn Trp 435 440 445 Glu Phe Ala Pro Asn Tyr Lys Leu Ser Phe Val Ala Ser His Gln Glu 450 455 460 Arg Leu Pro Leu Ala Gln Glu Leu Tyr Ala Asn Gly Gly His Phe Ala 465 470 475 480 Thr Asn Thr Tyr Glu Leu Gly Asn Asp Gln Leu Ser Lys Glu Lys Ser 485 490 495 Asn Asn Val Glu Leu Gly Leu His Phe Asp Asn Asp Lys Leu Asp Tyr 500 505 510 His Leu His Val Tyr His Asn Trp Phe Asp Asp Tyr Ile Tyr Ala Gln 515 520 525 Thr Leu Asp Arg Tyr Lys Asp Phe Arg Leu Val Gln Tyr Thr Gln Asp 530 535 540 Lys Ala Arg Phe Tyr Gly Ala Glu Gly Glu Ile Gly Tyr Gln Ile Thr 545 550 555 560 Pro Met Tyr Lys Ile Ser Ala Phe Gly Asp Tyr Val Arg Gly Lys Ile 565 570 575 Asp Ala Glu Gly Asn Ala Pro Arg Ile Pro Ala Gly Arg Leu Gly Thr 580 585 590 Lys Val Asp Ala Asp Phe Gly Asp Gly Phe Ser Gly Ser Ala Glu Tyr 595 600 605 Tyr His Val Phe Asn Gln Asp Lys Ile Ala Ala Tyr Glu Thr Glu Thr 610 615 620 Glu Gly Tyr Asn Met Leu Asn Leu Gly Val Ala Tyr Ser Gly Gln Tyr 625 630 635 640 Gly Ala Lys Thr Asp Tyr Arg Val Tyr Leu Lys Ala Asn Asn Leu Leu 645 650 655 Asp Asp Thr Val Tyr Gln His Ala Ser Phe Leu Ser Asn Ile Pro Gln 660 665 670 Val Gly Arg Asn Phe Thr Val Gly Val Asp Phe Ser Phe 675 680 685 10691PRTAcinetobacter calcoaceticus RUH2202 10Met Gln Phe Tyr Lys Asn Ile Leu Thr Leu Ser Ile Leu Ala Val Ile 1 5 10 15 Ser Val Pro Ser Phe Ala Ala Glu Ser Glu Asn Val Glu Lys Leu Glu 20 25 30 Thr Ile Arg Ile Lys Ala His Pro Leu Glu Gln Thr Ser Gln Asp Phe 35 40 45 Ala Val Ala Asp Thr Val Val Asp Gln Lys His Leu Thr Gln Gly Ala 50 55 60 Ala Thr Ile Gly Asp Ala Leu Ser Ser Glu Val Gly Ile Tyr Ala Asn 65 70 75 80 Gln Phe Gly Ala Gly Ser Ser Arg Pro Val Ile Arg Gly Gln Asp Gly 85 90 95 Pro Arg Val Lys Val Leu Gln Asn Ser Ser Glu Asn Ile Asp Val Ser 100 105 110 Thr Leu Ser Pro Asp His Ala Val Thr Val Asp Pro Val Leu Ala Lys 115 120 125 Gln Val Glu Val Ile Arg Gly Pro Ser Thr Leu Leu Phe Gly Ala Gly 130 135 140 Thr Val Gly Gly Leu Val Asn Val Ile Asp Asn Lys Ile Pro Thr Gln 145 150

155 160 Met Pro Glu Asn Gly Tyr Glu Gly Gln Val Gly Leu Arg Tyr Asn Thr 165 170 175 Gly Ser Asp Glu Lys Leu Ala Ser Ala Gly Val Thr Val Gly Leu Gly 180 185 190 Ser Gln Val Ala Leu Arg Val Glu Gly Leu Thr Arg Asp Ala Asn Asn 195 200 205 Tyr Ile Ala Pro Asn Tyr Ile His Glu Gly Glu Lys Glu Arg Arg Val 210 215 220 Asp Asn Thr Phe Ala Gln Gly Asp Ser Val Asn Val Gly Leu Ser Trp 225 230 235 240 Ile Tyr Asp Arg Gly Tyr Thr Gly Ile Ser Tyr Ser Asn Arg Arg Asp 245 250 255 Gln Tyr Gly Leu Pro Gly His Ser His Glu Tyr Glu Ser Cys Ser Ala 260 265 270 His Leu Thr Gly Ile Pro His Leu His Cys Gly Asp His Glu His Glu 275 280 285 Asp Gly Glu Glu Ala His Asp His Gly Asn Glu Glu His Glu His Glu 290 295 300 His Gly Gly Pro Trp Ile Asp Leu Lys Ser Glu Arg Tyr Asp Phe Lys 305 310 315 320 Thr Glu Leu Asn Asp Pro Phe Ala Gly Phe Gln Lys Leu Arg Ala Gln 325 330 335 Ala Ser Tyr Thr Asp Tyr Gln His Asp Glu Ile Glu Glu Gly Ala Ile 340 345 350 Ala Thr Arg Phe Gln Asn Lys Gly Tyr Asp Gly Arg Ile Glu Leu Val 355 360 365 His Asn Pro Ile Ala Asp Trp Glu Gly Val Ile Gly Ala Gln Val Gly 370 375 380 Gln Gln Lys Leu Asp Leu Thr Gly Glu Glu Ala Phe Met Ala Pro Thr 385 390 395 400 Thr Thr Lys Lys Trp Ser Val Phe Ala Leu Glu His Lys Gln Trp Lys 405 410 415 Asp Val His Phe Glu Leu Ser Ala Arg Ala Asp Gln Gln Glu Ile Asp 420 425 430 Val Glu Asp Asn Ser Lys Gln Asp Phe Asp Gly Ser Ala Phe Ser Tyr 435 440 445 Ala Gly Ala Ala Asn Trp Glu Phe Ala Pro Asn Tyr Lys Leu Ser Phe 450 455 460 Val Ala Ser His Gln Glu Arg Leu Pro Leu Ala Gln Glu Leu Tyr Ala 465 470 475 480 Asn Gly Gly His Phe Ala Thr Asn Thr Tyr Glu Leu Gly Asn Asp Gln 485 490 495 Leu Ser Lys Glu Lys Ser Asn Asn Val Glu Leu Gly Leu His Phe Asp 500 505 510 Asn Asp Lys Leu Asp Tyr His Leu His Val Tyr His Asn Trp Phe Asp 515 520 525 Asp Tyr Ile Tyr Ala Gln Thr Leu Asp Arg Tyr Lys Asp Phe Arg Leu 530 535 540 Val Gln Tyr Thr Gln Asp Lys Ala Arg Phe Tyr Gly Ala Glu Gly Glu 545 550 555 560 Ile Gly Tyr Gln Ile Thr Pro Met Tyr Lys Ile Ser Ala Phe Gly Asp 565 570 575 Tyr Val Arg Gly Lys Ile Asp Ala Glu Gly Asn Ala Pro Arg Ile Pro 580 585 590 Ala Gly Arg Leu Gly Thr Lys Val Asp Ala Asp Phe Gly Asp Gly Phe 595 600 605 Ser Gly Ser Ala Glu Tyr Tyr His Val Phe Asn Gln Asp Lys Ile Ala 610 615 620 Ala Tyr Glu Thr Glu Thr Glu Gly Tyr Asn Met Leu Asn Leu Gly Val 625 630 635 640 Ala Tyr Ser Gly Gln Tyr Gly Ala Lys Thr Asp Tyr Arg Val Tyr Met 645 650 655 Lys Ala Asn Asn Leu Leu Asp Asp Thr Val Tyr Gln His Ala Ser Phe 660 665 670 Leu Ser Asn Ile Pro Gln Val Gly Arg Asn Phe Thr Val Gly Val Asp 675 680 685 Phe Ser Phe 690 11680PRTAcinetobacter johnsonii SH046 11Met Ser Ser Met Ala Phe His Lys Asn Leu Ile Thr Leu Ser Ile Leu 1 5 10 15 Ala Val Val Ala Pro Ala Val Phe Ala Asp Asp Thr Thr Thr Gln Gln 20 25 30 Leu Glu Thr Leu Thr Ser Gln Ala His Pro Leu Val Gln Thr Ala Ala 35 40 45 Asp Phe Ala Val Ala Asp Gln Val Ile Glu Gln Lys Ser Leu Lys Glu 50 55 60 Arg Ala Pro Thr Ile Gly Asp Ala Leu Ala Asp Glu Leu Gly Val Tyr 65 70 75 80 Ser Asn Gln Tyr Gly Ser Gly Ser Ser Arg Pro Val Ile Arg Gly Gln 85 90 95 Asp Gly Pro Arg Val Lys Val Leu Gln His Ala Ser Glu Thr Ala Asp 100 105 110 Val Ser Thr Leu Ser Pro Asp His Ala Val Thr Val Asp Pro Ile Leu 115 120 125 Ala Lys Gln Ile Glu Ile Ile Arg Gly Pro Ser Thr Leu Leu Tyr Ser 130 135 140 Ala Gly Thr Val Gly Gly Leu Val Asn Val Thr Asp Gln Lys Ile Pro 145 150 155 160 Thr Ser Met Pro Glu Lys Gly Leu Glu Gly Thr Ala Gly Leu Arg Tyr 165 170 175 Asn Ser Gly Ser Asp Glu Lys Leu Ala Ser Ala Gly Ala Thr Val Ala 180 185 190 Leu Gly Ser Gln Phe Ala Leu Arg Val Glu Gly Ser Lys Arg Glu Ala 195 200 205 Asn Asp Tyr Ile Ala Pro Asn Tyr Phe His Glu His Glu Gly Glu Leu 210 215 220 Glu Lys Glu Arg Arg Val Gly Asn Thr Phe Ala Lys Gly Lys Thr Val 225 230 235 240 Ser Val Gly Gly Ser Trp Ile Gly Glu Arg Ser Phe Ala Gly Ile Ala 245 250 255 Tyr Thr Asn Arg Gln Asp Gln Tyr Gly Leu Pro Gly His Ser His Glu 260 265 270 Tyr Glu Ser Cys Thr Val Ser Gly Asn Leu Leu Ile Gly Cys Gly Glu 275 280 285 Glu Asp Tyr Gly Asp Glu His Glu Thr Gly Pro Trp Val Asp Leu Lys 290 295 300 Ser Glu Arg Tyr Asp Phe Arg Thr Glu Ile Leu Asn Pro Leu Ala Gly 305 310 315 320 Phe Glu Lys Leu Arg Ala His Ala Ser Tyr Thr Asp Tyr Gln His Asp 325 330 335 Glu Ile Glu Gly Asp Ser Val Ala Thr Thr Phe Lys Ser Lys Ser Tyr 340 345 350 Asp Ala Arg Leu Glu Met Val His Gln Pro Ile Ala Asp Trp Glu Gly 355 360 365 Val Val Gly Val Gln Tyr Asn Gln Gln Lys Leu Asp Ile Thr Gly Glu 370 375 380 Glu Ser Ile Leu Glu Pro Thr Lys Thr Gln Lys Trp Ser Val Phe Gly 385 390 395 400 Leu Glu His Lys Gln Trp Asn Asp Phe His Phe Glu Leu Gly Ala Arg 405 410 415 Val Asp Gln Gln Thr Ile Asp Ile Glu Ser Asp Arg Lys Asp Tyr Asp 420 425 430 Asp Tyr Ala Val Ser Tyr Ser Gly Ala Val Asn Trp Met Phe Ala Pro 435 440 445 Asn Tyr Lys Leu Ser Leu Val Gly Ser His Gln Glu Arg Leu Pro Leu 450 455 460 Ala Gln Glu Leu Tyr Ala Asp Gly Lys His Leu Ala Thr Asn Thr Tyr 465 470 475 480 Glu Arg Gly Asn Glu Asn Leu Asp Val Glu Lys Ser Asn Asn Leu Glu 485 490 495 Leu Gly Phe His Tyr Asp Thr Asp Lys Ile Asp Tyr His Val His Val 500 505 510 Tyr His Asn Trp Tyr Asp Asn Tyr Ile Tyr Ala Gln Thr Ala Asp Arg 515 520 525 Tyr Glu Asn Phe Arg Leu Val Asp Tyr Thr Gln Asp Lys Ala Arg Phe 530 535 540 Tyr Gly Thr Glu Ala Glu Ala Ser Tyr Ala Ile Asn Asp Val Tyr Lys 545 550 555 560 Met Ser Val Phe Gly Asp Tyr Val Arg Gly Lys Ile Asp Asn Asp Asn 565 570 575 Ala Pro Arg Val Pro Ala Gly Arg Leu Gly Thr Lys Val Asn Ala Asn 580 585 590 Phe Ser Asp Thr Trp Ser Gly Thr Ala Glu Tyr Tyr His Val Phe Glu 595 600 605 Gln Asp Gln Ile Ala Ser Tyr Glu Thr Glu Thr Glu Gly Tyr Asn Met 610 615 620 Val Asn Leu Gly Leu Ala Tyr Asn Gly Gln Tyr Met Gln Gly Asn Asp 625 630 635 640 Tyr Arg Val Tyr Phe Lys Ala Asn Asn Leu Leu Asp Glu Thr Val Tyr 645 650 655 Ser His Thr Ser Phe Leu Ser Thr Leu Pro Gln Val Gly Arg Asn Phe 660 665 670 Thr Ile Gly Leu Asp Phe Ser Phe 675 680 12685PRTAcinetobacter junii SH205 12Met Leu Thr Leu Ser Ile Leu Ala Ala Val Ser Val Thr Ala Phe Ala 1 5 10 15 Ala Glu Asn Glu Lys Val Glu Ser Leu Glu Thr Ile Arg Ile Lys Ala 20 25 30 His Pro Leu Glu Gln Thr Ser Gln Asp Phe Ala Val Ala Asp Ser Val 35 40 45 Ile Asp Gln Thr Lys Leu Ser Gln Gly Ala Val Thr Ile Gly Asp Val 50 55 60 Leu Gly Gln Gln Thr Gly Ile Tyr Ser Asn Gln Phe Gly Ala Gly Ser 65 70 75 80 Ser Arg Pro Val Ile Arg Gly Gln Asp Gly Ala Arg Val Lys Ile Leu 85 90 95 Gln Asn Ser Ser Glu Asn Ile Asp Val Ser Thr Leu Ser Pro Asp His 100 105 110 Ala Val Thr Val Asp Pro Ala Leu Ala Lys Gln Val Glu Val Ile Arg 115 120 125 Gly Pro Ser Thr Leu Leu Phe Gly Ala Gly Thr Val Gly Gly Leu Val 130 135 140 Asn Val Ile Asp Ser Lys Leu Pro Thr Gln Met Pro Glu Lys Gly Tyr 145 150 155 160 Glu Gly Asn Val Gly Leu Arg Tyr Asn Thr Gly Ser Asp Glu Lys Leu 165 170 175 Ala Ser Ala Gly Val Thr Val Gly Leu Gly Asp Gln Val Ala Leu Arg 180 185 190 Val Glu Gly Leu Lys Arg Asp Ala Asn Asn Tyr Ile Ala Pro Asp Tyr 195 200 205 Phe His Glu Gly Glu Lys Glu Arg Arg Val Asp Asn Thr Phe Ala Glu 210 215 220 Gly Gln Thr Val Asn Val Gly Leu Ser Trp Ile Tyr Asp Arg Gly Phe 225 230 235 240 Thr Gly Ile Ser Tyr Ser Asn Arg Gln Asp Lys Tyr Gly Leu Pro Gly 245 250 255 His Ser His Glu Tyr Glu Ser Cys Glu Ala His Leu Val Gly Thr Pro 260 265 270 His Leu His Cys Gly Asp His Glu His Glu Asp Glu His Gly Gly Asp 275 280 285 His Tyr Asp Glu His Glu Gly Glu His Val His Glu Ala Gly Pro Trp 290 295 300 Ile Asp Leu Lys Ser Glu Arg Tyr Asp Ile Arg Ser Glu Leu Asp Asn 305 310 315 320 Pro Phe Lys Gly Phe Lys Lys Phe Arg Ala Gln Ala Ser Tyr Thr Asp 325 330 335 Tyr Gln His Asp Glu Ile Glu Glu Asp Thr Ile Ala Thr Arg Phe Lys 340 345 350 Asn Lys Ala Tyr Asp Gly Arg Val Glu Leu Val His Asn Pro Ile Gly 355 360 365 Ala Trp Glu Gly Val Ile Gly Thr Gln Tyr Gly Gln Gln Lys Leu Asn 370 375 380 Leu Thr Gly Glu Glu Ala Phe Leu Ala Pro Asn Thr Thr Lys Lys Trp 385 390 395 400 Ser Leu Phe Ala Leu Glu His Ala Gln Phe Asn Asp Val His Val Glu 405 410 415 Leu Ala Ala Arg Val Asp Gln Gln Lys Ile Asp Ile Glu Asp Ser Ser 420 425 430 Lys Lys Asp Phe Asp Gly Ser Ala Phe Ser Ala Ser Gly Ala Ala Asn 435 440 445 Trp Glu Phe Ala Pro Asp Tyr Lys Leu Ser Leu Val Ala Ser His Gln 450 455 460 Gln Arg Leu Pro Leu Ala Gln Glu Leu Tyr Ala Asp Gly Lys His Phe 465 470 475 480 Ala Thr Asn Thr Tyr Glu Leu Gly Asn Asp Gln Leu Lys Lys Glu Gln 485 490 495 Ser Asn Asn Val Glu Leu Gly Phe His Tyr Asp Asn Asn Arg Phe Asp 500 505 510 Tyr His Ile His Val Tyr His Asn Trp Phe Asp Asp Tyr Ile Tyr Ala 515 520 525 Gln Thr Leu Asp Arg Tyr Glu Asp Phe Arg Leu Val Lys Tyr Thr Gln 530 535 540 Asp Lys Ala Arg Phe Tyr Gly Ala Glu Ala Glu Ala Ala Tyr Gln Ile 545 550 555 560 Ser Pro Thr Tyr Lys Ile Gly Val Phe Gly Asp Tyr Val Arg Gly Lys 565 570 575 Ile Asp Asn Glu Asn Ala Pro Arg Val Pro Ala Gly Arg Leu Gly Thr 580 585 590 Lys Val Asn Ala Asp Phe Gly Asp Gly Phe Thr Gly Ser Ala Glu Tyr 595 600 605 Tyr His Val Phe Asn Gln Asp Lys Ile Thr Ala Tyr Glu Thr Glu Thr 610 615 620 Gln Gly Tyr Asn Met Leu Asn Leu Gly Val Ala Tyr Ser Gly Glu Tyr 625 630 635 640 Ser Asn Leu Gly Asn Tyr Arg Val Phe Leu Asn Ala Asn Asn Leu Leu 645 650 655 Asp Asp Gln Ile Tyr Gln His Ala Ser Phe Leu Ser Thr Ile Pro Gln 660 665 670 Val Gly Arg Asn Phe Thr Val Gly Val Asn Phe Asn Phe 675 680 685 13684PRTAcinetobacter lwoffii SH145 13Met Ser Ile Phe Ala Val Val Ala Pro Thr Val Phe Ala Glu Gln Ser 1 5 10 15 Ser Ser Ser Ile Pro Val Gln Thr Met Asp Thr Ile Gln Val Gln Ala 20 25 30 His Pro Leu Val Gln Thr Ala Ala Asp Phe Ala Val Ala Asp His Phe 35 40 45 Val Asp Gln Lys Ala Leu Ser Glu Arg Ala Pro Thr Ile Gly Asp Ala 50 55 60 Leu Ala Asp Glu Leu Gly Val Tyr Ser Asn Gln Tyr Gly Ser Gly Ser 65 70 75 80 Ser Arg Pro Val Ile Arg Gly Gln Asp Gly Pro Arg Val Lys Val Leu 85 90 95 Gln His Ala Ser Glu Thr Ala Asp Val Ser Thr Leu Ser Pro Asp His 100 105 110 Ala Val Thr Val Asp Pro Ile Leu Ala Lys Gln Val Glu Val Ile Arg 115 120 125 Gly Pro Ser Thr Leu Leu Tyr Gly Ala Gly Thr Val Gly Gly Leu Val 130 135 140 Asn Val Thr Asp Gln Lys Ile Pro Thr Gln Met Pro Glu Asp Gly Leu 145 150 155 160 Glu Gly Thr Val Gly Leu Arg Tyr Asn Ser Gly Ser Asp Glu Lys Leu 165 170 175 Ala Ser Ala Gly Val Thr Ala Gly Ile Gly Glu Asn Phe Ala Leu Arg 180 185 190 Val Glu Gly Ser Lys Arg Asn Ala Asn Asp Tyr Ile Ala Pro Asp Tyr 195 200 205 Phe His Glu His Asp Asp Glu Leu Glu Lys Glu Arg Arg Val Gly Asn 210 215 220 Thr Phe Ala Glu Gly Gln Thr Val Asn Ile Gly Gly Ser Trp Ile His 225 230 235 240 Asp Arg Gly Phe Val Gly Leu Ser Tyr Ser Asn Arg Gln Asp Gln Tyr 245 250 255 Gly Leu Pro Gly His Ser His Glu Tyr His Gly Cys Glu Ile His Gly 260 265 270 Asp His Phe His Cys Pro Lys Pro Gly Glu Asp Glu His Asp His Glu 275 280 285 Glu Thr Ala Gly Pro Trp Val Asp Leu Lys Ser Glu Arg Tyr Asp Val 290 295 300 Arg Thr Glu Leu Glu Gln Pro Phe Ala Gly Phe Glu Lys Leu Arg Ala 305 310 315 320 His Ala Ser Phe Thr Asp Tyr Glu His Asp Glu Leu Glu Glu Asn Ala 325 330 335 Val Ile Ser Asn Phe Lys Ser Lys Gly Tyr Asp Ala Arg Leu Glu Leu 340 345 350 Val His Val Pro Val Ala Gly Trp Glu Gly Val Ile Gly Thr Gln Tyr 355 360 365 Ser Gln Gln Lys Ile Asp Leu Ser Gly Gln Leu Glu Pro His Asp Asp 370 375 380 His Phe His Glu Ala Val Val Met Pro Asp Thr Lys Thr Asp Lys Phe 385

390 395 400 Ser Leu Phe Ala Leu Glu His Lys Gln Leu Gly Asp Val His Val Glu 405 410 415 Leu Gly Ala Arg Val Asp His Gln Lys Val Lys Val Asp Ser Asp Gln 420 425 430 Lys Asp Tyr Ser Gly Thr Gly Val Ser Ala Ser Ala Ala Ala Asn Trp 435 440 445 Glu Phe Ala Pro Asn Tyr Lys Leu Ser Val Val Gly Ser His Gln Gln 450 455 460 Arg Leu Pro Leu Ala Gln Glu Leu Tyr Ala Asp Gly Leu His Phe Ala 465 470 475 480 Thr Asn Thr Tyr Glu Leu Gly Asn Pro Asp Leu Asp Lys Glu Thr Ser 485 490 495 Asn Asn Leu Glu Leu Gly Leu His Tyr Glu Gly Asp Lys Leu Asp Tyr 500 505 510 His Val His Val Tyr His Asn Trp Phe Asp Asp Tyr Ile Tyr Gly Glu 515 520 525 Thr Val Ala Gln Lys Gly Asn Leu Arg Gly Val Gln Tyr Thr Gln Asp 530 535 540 Lys Ala Arg Phe Tyr Gly Thr Glu Val Gln Ala Gly Tyr Gln Ile Asn 545 550 555 560 Asp Met Tyr Lys Leu Ser Val Phe Gly Asp Tyr Val Arg Gly Lys Ile 565 570 575 Glu Ala Glu Asn Ala Pro Arg Val Pro Ala Gly Arg Leu Gly Thr Lys 580 585 590 Val Glu Ala Asp Phe Ala Asp Gly Trp Ser Gly Leu Ala Glu Tyr Tyr 595 600 605 His Ile Phe Asn Gln Asp Lys Ile Ala Ser Tyr Glu Asp Glu Thr Gln 610 615 620 Gly Tyr Asn Met Val Asn Val Gly Leu Ser Tyr Ala Asn Ser Ile Ala 625 630 635 640 Asp Asn Asn Ala Tyr Arg Val Tyr Phe Lys Ala Asn Asn Leu Leu Asp 645 650 655 Asp Gln Val Tyr Ser His Thr Ser Phe Leu Ser Asn Ile Pro Gln Val 660 665 670 Gly Arg Asn Phe Thr Val Gly Val Gln Tyr Asp Phe 675 680 14699PRTAcinetobacter radioresistens SK82 14Met Gln Phe Ser Lys Asn Phe Leu Thr Leu Ser Ile Met Ala Val Val 1 5 10 15 Ser Pro Val Ile Phe Ala Glu Glu Asn Ser Ser Val Gln Gln Leu Glu 20 25 30 Thr Ile Gln Val Thr Ala His Pro Leu Val Gln Ser Ala Val Asp Tyr 35 40 45 Val Ala Ala Asp Asn Ile Ile Glu Lys Glu Gln Leu Ile Gln Gly Gly 50 55 60 Thr Thr Ile Gly Glu Ala Leu Ser Asp Gln Val Gly Val Tyr Ser Asn 65 70 75 80 Gln Phe Gly Pro Gly Ala Ser Arg Pro Val Ile Arg Gly Gln Glu Gly 85 90 95 Ala Arg Val Lys Val Leu Gln Asn Ala Ser Glu Thr Met Asp Val Ser 100 105 110 Thr Leu Ser Pro Asp His Ala Val Met Val Asp Pro Ala Leu Ala Lys 115 120 125 Gln Val Glu Ile Val Arg Gly Pro Ser Thr Leu Leu Tyr Gly Ala Gly 130 135 140 Thr Val Gly Gly Leu Val Asn Val Thr Asp Ser Lys Ile Pro Thr Arg 145 150 155 160 Ile Pro Asp Asn Gly Tyr Glu Gly Arg Ala Gly Leu Arg Tyr Asn Ser 165 170 175 Gly Asn Asp Glu Lys Leu Glu Thr Ala Gly Val Thr Leu Ala Leu Gly 180 185 190 Glu Gln Val Ala Leu Arg Val Glu Gly Leu Lys Arg Asp Ala Asn Asn 195 200 205 Tyr Ile Ala Pro Asp Tyr Tyr His Glu His Ser His Gly Asp His Ser 210 215 220 His Leu Val Lys Glu Arg Arg Val Asp Asn Thr Phe Ala Gln Ser Asp 225 230 235 240 Thr Ala Ser Val Gly Leu Ser Trp Ile His Asp Arg Gly Phe Thr Gly 245 250 255 Leu Ser Tyr Thr Asn Arg Gln Asp Gln Tyr Gly Leu Pro Gly His Ser 260 265 270 His Glu Tyr Glu Ser Cys Glu Ala Tyr Leu Ser Gly Arg Pro His Leu 275 280 285 His Cys Gly Asp Glu His His His Asp Glu Glu Gly His Glu His Glu 290 295 300 His Ser His Glu Lys Asp His Asp His Ala Gly Pro Trp Val Asp Leu 305 310 315 320 Lys Ser Glu Arg Tyr Asp Phe Arg Thr Glu Leu Asp Asp Pro Phe Ala 325 330 335 Gly Phe Lys Lys Leu Arg Ala Gln Ala Ser Tyr Thr Asp Tyr Lys His 340 345 350 Asp Glu Ile Glu Glu Gly Glu Ala Ala Thr Thr Phe Lys Ser Thr Gly 355 360 365 Tyr Asp Ala Arg Leu Glu Leu Val His Gln Pro Val Ala Ala Trp Glu 370 375 380 Gly Val Ile Gly Thr Gln Phe Gly Arg Gln Lys Leu Asn Ile Thr Gly 385 390 395 400 Glu Glu Ser Leu Phe Ala Gly Pro Thr Thr Thr Asp Lys Trp Ser Leu 405 410 415 Phe Ala Leu Glu His Thr Gln Trp Asn Asp Val His Phe Glu Leu Ala 420 425 430 Ala Arg Phe Asp Gln Gln Lys Ile Glu Ile Asp Ser Pro Gln Lys Asn 435 440 445 Tyr Asp Asp His Ala Phe Ser Tyr Ser Gly Ala Ala Asn Trp Glu Phe 450 455 460 Leu Pro Asp Tyr Lys Leu Ser Leu Val Ala Ser His Gln Glu Arg Leu 465 470 475 480 Pro Leu Ala Gln Glu Leu Tyr Ala Lys Gly Lys His Leu Ala Thr Asn 485 490 495 Thr Tyr Glu Ile Gly Asn Glu Asn Leu Asp Thr Glu Lys Ser Asn Asn 500 505 510 Ile Glu Leu Gly Phe His Tyr Glu Gly Asp Lys Leu Asn Tyr His Ile 515 520 525 His Ala Tyr His Asn Trp Phe Asp Asp Tyr Ile Tyr Ala Gln Thr Leu 530 535 540 Asp Arg Phe Glu Asn Phe Arg Leu Val Glu Tyr Thr Gln Asp Lys Ala 545 550 555 560 Arg Phe Tyr Gly Ala Glu Gly Glu Val Ser Tyr Gln Leu Ser Pro Arg 565 570 575 Tyr Lys Ala Ser Leu Phe Gly Asp Tyr Val Arg Gly Lys Ile Glu Thr 580 585 590 Glu Gly Asn Ala Pro Arg Ile Pro Gly Gly Arg Leu Gly Ser Lys Ile 595 600 605 Asp Ala Asp Phe Gly Asp Gly Phe Ser Gly Met Ala Glu Tyr Tyr His 610 615 620 Val Phe Thr Gln Asp Lys Ile Ala Ala Tyr Glu Thr Glu Thr Glu Gly 625 630 635 640 Tyr Asn Met Val Asn Leu Gly Leu Ala Tyr Ser Ala Arg Ala Thr Asp 645 650 655 Lys Thr Asp Tyr Arg Ile Tyr Val Lys Ala Asn Asn Leu Leu Asp Glu 660 665 670 Thr Val Tyr Asn His Ala Ser Phe Leu Ser Asn Ile Pro Gln Val Gly 675 680 685 Arg Asn Phe Thr Val Gly Leu Asp Phe Asn Phe 690 695 15693PRTAcinetobacter sp. ADP1 15Met Leu Phe Thr Arg Ser Val Leu Ala Leu Ser Ile Phe Ala Ala Leu 1 5 10 15 Ser Ser Val Val His Ala Ala Asn Glu Thr Asn Thr Val Ser Lys Leu 20 25 30 Lys Thr Ile Glu Ala Thr Ala His Pro Leu Val Gln Thr Ala Val Asp 35 40 45 Tyr Ala Ala Ala Asp Asn Ile Ile Gln Asn Gln Gln Leu Val Gln Gly 50 55 60 Gly Ala Thr Ile Gly Glu Ala Leu Ser Asp Gln Val Gly Val Tyr Ser 65 70 75 80 Asn Gln Phe Gly Pro Gly Ala Ser Arg Pro Val Ile Arg Gly Gln Asp 85 90 95 Ser Ala Arg Val Lys Val Leu Gln Asn Ala Ser Glu Thr Ile Asp Val 100 105 110 Ser Thr Leu Ser Pro Asp His Ala Val Met Val Asn Pro Ile Leu Ala 115 120 125 Lys Gln Val Glu Ile Val Arg Gly Pro Ser Thr Leu Leu Tyr Gly Ala 130 135 140 Gly Thr Val Gly Gly Leu Val Asn Val Thr Asp Asn Lys Ile Pro Thr 145 150 155 160 Gln Met Pro Glu Lys Gly Tyr Glu Gly Gln Val Gly Leu Arg Tyr Asn 165 170 175 Thr Gly Ser Asp Glu Lys Leu Gly Ser Ala Gly Leu Thr Val Gly Leu 180 185 190 Gly Glu Gln Val Ala Leu Arg Leu Glu Gly Leu Lys Arg Asp Ala Asn 195 200 205 Asn Tyr Ile Ala Pro Asn Tyr Leu His Glu Gly Glu Lys Glu Arg Arg 210 215 220 Val Asp Asn Thr Phe Ala Lys Ser Glu Asp Thr Thr Ile Gly Leu Ser 225 230 235 240 Trp Ile His Asp Arg Gly Phe Ala Gly Ile Ser Tyr Thr Asn Arg Gln 245 250 255 Asp Lys Tyr Gly Leu Pro Gly His Ser His Glu Tyr Glu Ser Cys His 260 265 270 Pro His Gly Leu Lys Leu His Cys Gly Ser His Asp Asp His Asp His 275 280 285 Gly Asp His Asp Glu Glu Glu Gly His Asp His Asp His Glu His Ala 290 295 300 His Asp His Ala Gly Pro Trp Ile Asn Leu Lys Ser Glu Arg Tyr Asp 305 310 315 320 Phe Arg Thr Glu Leu Asn Asp Pro Phe Ser Gly Phe Ser Lys Leu Arg 325 330 335 Ala Gln Ala Ser Tyr Thr Asp Tyr Lys His Asp Glu Ile Glu Glu Gly 340 345 350 Glu Ala Ala Thr Thr Phe Lys Ser Gln Gly Tyr Asp Ala Arg Leu Glu 355 360 365 Leu Val His Gln Pro Leu Ala Asn Trp Glu Gly Val Val Gly Ala Gln 370 375 380 Phe Gly Arg Gln Lys Leu Asp Ile Thr Gly Glu Glu Ser Leu Phe Ala 385 390 395 400 Gly Pro Ser Thr Thr Asp Lys Trp Ser Leu Phe Ala Leu Glu His Thr 405 410 415 Gln Trp Asn Asp Val His Phe Glu Val Ala Ala Arg Phe Asp Gln Gln 420 425 430 Lys Ile Asp Ile Asp Ser Ala Gln Lys Asn Tyr Asp Asp His Ala Phe 435 440 445 Ser Tyr Ser Gly Ala Ala Asn Trp Ala Phe Ala Pro Asn Tyr Lys Leu 450 455 460 Ser Phe Val Ala Ser His Gln Glu Arg Met Pro Leu Ala Gln Glu Leu 465 470 475 480 Tyr Ala Asn Gly Lys His Leu Ala Thr Asn Thr Tyr Glu Leu Gly Asn 485 490 495 Gln Asn Leu Ser Thr Glu Lys Ser Asn Asn Leu Glu Leu Gly Phe His 500 505 510 Tyr Asp Ala Asp Lys Leu Asn Tyr His Val His Val Phe His Asn Trp 515 520 525 Phe Asp Asp Tyr Ile Tyr Ala Arg Thr Leu Asp Arg Phe Glu Asn Phe 530 535 540 Arg Leu Val Glu Tyr Thr Gln Asp Lys Ala Arg Phe Tyr Gly Val Glu 545 550 555 560 Gly Glu Leu Ser Tyr Gln Leu Ser Pro Arg Tyr Thr Ala Thr Val Phe 565 570 575 Gly Asp Tyr Val Arg Ala Lys Ile Glu Thr Glu Gly Asn Ala Pro Arg 580 585 590 Ile Pro Gly Gly Arg Leu Gly Thr Arg Ile Asn Ala Asp Phe Gly Asp 595 600 605 Gly Phe Ser Gly Leu Ala Glu Tyr Phe His Val Phe Lys Gln Asp Lys 610 615 620 Phe Ala Ala Phe Glu Asn Glu Thr Ala Gly Tyr Asn Met Val Asn Leu 625 630 635 640 Gly Leu Ala Tyr Ala Ala Arg Ala Thr Asn Arg Thr Asp Tyr Arg Val 645 650 655 Tyr Val Lys Ala Asn Asn Ile Leu Asp Glu Thr Val Tyr Asn His Ala 660 665 670 Ser Phe Leu Ser Asn Leu Pro Gln Val Gly Arg Asn Phe Thr Val Gly 675 680 685 Val Asp Phe Ser Phe 690 16696PRTAcinetobacter sp. ATCC 27244 16Met Ser Phe Ser Lys Asn Ile Leu Thr Leu Ser Ile Leu Ala Ala Val 1 5 10 15 Ser Val Ser Ala Leu Ala Ala Glu Asn Gln Lys Val Gln Thr Leu Glu 20 25 30 Thr Ile Arg Ile Lys Ala His Pro Leu Glu Gln Thr Ser Gln Asp Phe 35 40 45 Ala Val Ala Asp Thr Val Val Asp Gln Lys Thr Leu Thr Glu Gly Ala 50 55 60 Val Thr Ile Gly Asp Ala Leu Ala Ser Glu Val Gly Ile Tyr Ser Asn 65 70 75 80 Gln Phe Gly Ala Gly Ser Ser Arg Pro Val Ile Arg Gly Gln Asp Gly 85 90 95 Ala Arg Val Lys Val Leu Gln Asn Ser Ser Glu Asn Ile Asp Val Ser 100 105 110 Thr Leu Ser Pro Asp His Ala Val Thr Val Asp Pro Ala Leu Ala Thr 115 120 125 Gln Val Glu Val Ile Arg Gly Pro Ser Thr Leu Leu Phe Gly Ala Gly 130 135 140 Thr Val Gly Gly Leu Val Asn Val Asn Asp Ser Lys Leu Pro Thr Lys 145 150 155 160 Met Pro Glu Lys Gly Tyr Glu Gly Asn Val Gly Leu Arg Tyr Asn Thr 165 170 175 Gly Ser Asp Glu Lys Leu Ala Ser Ala Gly Val Thr Val Gly Leu Gly 180 185 190 Asp Gln Phe Ala Leu Arg Val Glu Gly Leu Lys Arg Glu Ala Asn Asp 195 200 205 Tyr Ile Ala Pro Asn Tyr Phe His Glu Gly Glu Lys Glu Arg Arg Val 210 215 220 Asp Asn Thr Phe Ala Glu Gly Gln Thr Val Asn Val Gly Leu Ser Trp 225 230 235 240 Ile Tyr Asp Arg Gly Phe Thr Gly Ile Ser Tyr Ser Asn Arg Gln Asp 245 250 255 Lys Tyr Gly Leu Pro Gly His Ser His Glu Tyr Glu Ser Cys Asp Ala 260 265 270 His Leu Val Gly Arg Pro His Leu His Cys Gly Asp His Gly His Asp 275 280 285 His Gly His Asp Asp His Asp Asp Asp His Gly His Asp Asp His Asp 290 295 300 His Asp His Glu His Ala His Gly Gly Pro Trp Ile Asp Leu Lys Ser 305 310 315 320 Glu Arg Tyr Asp Phe Arg Thr Glu Leu Asp Asp Pro Phe Ala Gly Phe 325 330 335 Lys Lys Met Arg Ala Gln Ala Ser Tyr Thr Asp Tyr Arg His Asp Glu 340 345 350 Ile Glu Glu Asp Thr Ile Thr Thr Arg Phe Lys Asn Lys Gly Tyr Asp 355 360 365 Gly Arg Phe Glu Leu Val His Asn Pro Leu Gly Ala Trp Glu Gly Val 370 375 380 Ile Gly Thr Gln Tyr Ser Gln Gln Lys Leu Thr Leu Thr Gly Gln Glu 385 390 395 400 Ala Phe Leu Ala Pro Asn Thr Thr Lys Lys Trp Ser Val Phe Ala Leu 405 410 415 Glu His Ala Gln Phe Asn Asp Val His Val Glu Leu Ala Ala Arg Ala 420 425 430 Asp Lys Gln Lys Ile Asp Ile Asp Asp Ser Ser Lys Gln Asp Phe Asp 435 440 445 Gly Ser Ala Phe Ser Val Ser Gly Ala Ala Asn Trp Glu Phe Ala Pro 450 455 460 Asn Tyr Lys Leu Ser Phe Val Thr Ser His Gln Gln Arg Leu Pro Leu 465 470 475 480 Ala Gln Glu Leu Tyr Ala Asn Gly Lys His Phe Ala Thr Asn Thr Tyr 485 490 495 Glu Leu Gly Asn Asp Gln Leu Lys Lys Glu Lys Ser Asn Asn Val Glu 500 505 510 Leu Gly Phe His Tyr Asp Asp Asn Ile Phe Asp Tyr His Val His Val 515 520 525 Tyr His Asn Trp Phe Asp Asp Tyr Ile Phe Ala Gln Thr Leu Asp Arg 530 535 540 Tyr Glu Asp Phe Arg Leu Val Glu Tyr Thr Gln Asp Lys Ala Arg Phe 545 550 555 560 Tyr Gly Ala Glu Ala Glu Ala Gly Tyr Gln Val Ser Pro Ile Tyr Lys 565 570 575 Val Ser Val Phe Gly Asp Tyr Val Arg Gly Lys Ile Asp Asn Glu Asn 580 585 590 Ala Pro Arg Val Pro Ala Gly Arg Leu Gly Thr Lys Val Lys Ala Asn 595 600 605 Phe Gly Asp Gly

Phe Ser Gly Ser Ala Glu Tyr Tyr His Val Phe Gln 610 615 620 Gln Asp Lys Ile Ala Ala Tyr Glu Thr Asp Thr Gln Ser Tyr Asn Met 625 630 635 640 Val Asn Leu Gly Val Ala Tyr Ser Gly Gln Tyr Ser Ser Ala Gly Asp 645 650 655 Tyr Arg Val Phe Leu Asn Ala Asn Asn Leu Leu Asp Glu Lys Val Tyr 660 665 670 Gln His Ala Ser Phe Leu Ser Thr Val Pro Gln Val Gly Arg Asn Phe 675 680 685 Thr Val Gly Val Asn Phe Ser Phe 690 695 17670PRTAcinetobacter sp. RUH2624 17Met Gln Leu Lys Thr Gln Leu Phe Val Leu Leu Leu Leu Ser Leu Ser 1 5 10 15 Ile Gln Gln Val Met Ala Ala Asp Asp Thr Asn Thr Lys Glu Glu Lys 20 25 30 Pro Ala Glu Leu Ala Thr Ile Thr Val Lys Ala Glu Pro Thr Ala Pro 35 40 45 Ala Asn Asn Thr Ser Leu Ala Glu Gly Ala Thr Ser Ile Gly Asn Ala 50 55 60 Leu Asn Gly Gln Ala Gly Val Tyr Ser Ala Gln Tyr Thr Gly Gly Ala 65 70 75 80 Ser Arg Pro Val Ile Arg Gly Gln Asp Gly Thr Arg Val Lys Ile Val 85 90 95 Gln Asn Gly Gly Asp Val Met Asp Val Ser Ser Val Ser Pro Asp His 100 105 110 Ala Val Thr Val Asp Pro Asn Ser Ala Gln Asp Ile Gln Ile Leu Asn 115 120 125 Gly Ala Glu Ala Leu Leu Tyr Gly Ala Gly Ser Val Gly Gly Leu Val 130 135 140 Asn Val Val Asp Glu Lys Ile Pro Thr Ser Met Pro Asp Lys Gly Tyr 145 150 155 160 Gln Gly Lys Ala Gly Val Arg Tyr Asn Ser Gly Ser Asp Glu Leu Leu 165 170 175 Tyr Ser Gly Gln Ala Thr Val Gly Leu Gly Asp His Val Ala Leu Arg 180 185 190 Val Gly Gly Leu Lys Arg Asp Ala Asn Asp Tyr Ile Leu Pro Arg Asp 195 200 205 Leu Gln Thr Asp Glu Arg Arg Gln Asp Ser Thr Phe Ala Asp Ser Lys 210 215 220 Asn Tyr Asn Ala Gly Leu Ser Trp Ile Gly Asp Arg Gly Phe Ile Gly 225 230 235 240 Ala Ser Phe Ser Gln Arg His Asp Gln Tyr Gly Ile Pro Ala Asp Asn 245 250 255 Glu Leu Phe Gly Ser Cys Glu Arg Asp Gly Leu His Leu Val Cys Gly 260 265 270 Thr Asp Asp Pro Ser Thr Asp His Glu His Glu His Glu His Asp Ala 275 280 285 Ser Trp Ile Asn Leu Lys Glu Lys Arg Tyr Asp Leu Lys Gly Glu Leu 290 295 300 Arg Asp Pro Phe Ala Gly Phe Ala Lys Val Ala Ala Gln Ala Ser Tyr 305 310 315 320 Thr Asp Tyr Gln His Glu Glu Met His Asn Thr Asp Val Gly Thr Thr 325 330 335 Phe Lys Ser Lys Gly Ile Asp Ser Arg Val Thr Leu Glu Asn Asn Ala 340 345 350 Trp Ala Gly Trp Thr Gly Gln Ile Gly Ala Gln Tyr Thr Gln Gln Lys 355 360 365 Leu Asn Ile Val Gly Asp Glu Ser Ile Met Asp Pro Ser Lys Thr Gln 370 375 380 Arg Tyr Ser Val Phe Gly Leu Gln Gln Lys Gln Ile Asn Asn Val Asn 385 390 395 400 Leu Ala Val Ser Ser Arg Ile Asp His Gln Thr Ile Asp Ile Glu Ser 405 410 415 Asp Gln Lys Asn Tyr Thr Gly Thr Gly Tyr Ser Val Ala Gly Thr Ala 420 425 430 Ser Trp Glu Phe Ile Pro Gln Tyr Lys Leu Ser Leu Thr Thr Ser His 435 440 445 Gln Glu Arg Leu Pro Phe Ala Gln Glu Leu Tyr Ser Asp Gly Val His 450 455 460 Met Ala Thr Asn Thr Tyr Glu Leu Gly Asn Asp Asp Leu Lys Lys Glu 465 470 475 480 Arg Ser Asn Asn Val Glu Leu Gly Leu His Phe Asn Asn Asp Leu Leu 485 490 495 Lys Tyr Asn Val Ser Val Phe His Asn Arg Phe Asp Asn Phe Ile Tyr 500 505 510 Ala Asn Thr Leu Asp Asp Phe Gln Gly Phe Arg Leu Ile Gln Tyr Ser 515 520 525 Gln Asp Ala Ala Lys Phe Tyr Gly Val Asp Ala Asp Leu Ser Tyr Gln 530 535 540 Ile Ser Pro Val Tyr Asn Leu Gly Leu Phe Gly Asp Tyr Val Arg Gly 545 550 555 560 Lys Ile Asp Asn Glu Asn Ala Pro Arg Ile Pro Gly Gly Arg Leu Gly 565 570 575 Thr Lys Val Lys Ala Asp Phe Gly Asp Gly Trp Asp Gly Ser Ala Glu 580 585 590 Tyr Tyr His Val Phe Lys Gln Asp Asp Ile Ala Asn Tyr Glu Thr Lys 595 600 605 Gly Gln Ser Tyr Asn Met Leu Asn Leu Gly Val Gly Tyr Asn Gly Lys 610 615 620 Tyr Ser Asn Ile Gly Asp Tyr Arg Val Phe Leu Asn Ala Asn Asn Leu 625 630 635 640 Leu Asp Ser Arg Ile Tyr Gln His Glu Ser Phe Leu Ala Asn Val Pro 645 650 655 Gln Val Gly Arg Asn Phe Thr Val Gly Val Asn Phe Ser Phe 660 665 670 18823PRTActinobacillus minor 202 18Met Lys Lys Asn Ala Ile Thr Leu Ser Met Ile Ser Leu Phe Ser Val 1 5 10 15 Cys Ala Tyr Ala Glu Glu Gly Ala Glu Leu Glu Glu Ile Gln Val Glu 20 25 30 Thr Lys Met Ala Glu Ser Asn Leu Leu Gly Asp Arg Pro Asn Val Ser 35 40 45 Asp Lys Leu Ile Asp Gly Lys Val Phe Lys Gln Lys Ser Thr Thr Leu 50 55 60 Gly Asp Ala Leu Ser Ser Glu Leu Gly Ile His Ser Asn Gln Tyr Gly 65 70 75 80 Gly Gly Ala Ser Ala Pro Ile Ile Arg Gly Gln Glu Gly Lys Arg Ile 85 90 95 Lys Ile Leu Gln Asn Asn Ser Asp Val Val Asp Met Ser Ile Met Ser 100 105 110 Pro Asp His Ala Val Thr Val Asp Thr Thr Leu Ser Lys Gln Val Glu 115 120 125 Ile Val Arg Gly Pro Ser Thr Leu Leu Tyr Ser Ser Gly Asn Ala Ala 130 135 140 Gly Val Ile Asn Val Leu Asp Asn Lys Ile Pro Asn Phe Met Pro Gln 145 150 155 160 Asn Gly Leu Lys Gly Glu Val Gly Phe Arg Phe Asn Thr Asn Asn Asn 165 170 175 Glu Lys Leu Thr Thr Ala Gly Val Thr Phe Ala Ile His Pro Ser Ile 180 185 190 Ala Val His Leu Glu Gly Leu Ser Lys Gln Ala Gly Asn Tyr Lys Thr 195 200 205 Pro His Tyr Arg Tyr Gly Thr Tyr Ala Asp Lys Lys Ala Phe Asn Arg 210 215 220 Arg Glu Met Ser Tyr Gln Asn Leu Ser His Val Pro Glu Ser Trp Ala 225 230 235 240 Lys Ser Gln Val Gly Thr Ala Gly Ile Ser Trp Val Tyr Glu Lys Gly 245 250 255 Tyr Leu Gly Leu Ala Tyr Thr Glu Arg Gln Asp Lys Tyr Gly Leu Pro 260 265 270 Ala His Asn His Ile Tyr Glu Gly Cys Ala Val Arg Ile Ile Asp Glu 275 280 285 Ser Val Lys Gln Gln Tyr Pro Tyr Leu Phe Pro Tyr Pro Glu Leu Ala 290 295 300 Asp Asp Arg His Leu Phe Trp Ala Asn Pro Gly Val Asn Met Ala Asp 305 310 315 320 Cys His Ala His Gly Leu Ser Ala Thr Pro Tyr Val Asp Leu Lys Ser 325 330 335 Gln Arg Tyr Asp Phe Arg Gly Glu Ile Ile Glu Pro Phe Lys Tyr Val 340 345 350 Asp Lys Leu Arg Phe Asn Ala Ser His Val Asn Tyr Gln His Gly Glu 355 360 365 Ile Glu Gly Glu Lys Ala Ala Asn Leu Phe Lys Asn Lys Gly Leu Thr 370 375 380 Thr Arg Leu Glu Phe Val His Ser Pro Val Gly Asn Leu Thr Gly Ile 385 390 395 400 Trp Gly Ile Gln Tyr Leu Glu Gln Lys Asn Ser Ala Leu Ser Pro Glu 405 410 415 Asp Ser Ala Thr His Lys His Arg Gly Val Gln Gln Leu Leu Asn Asn 420 425 430 Asn Lys Met Gln Asn Trp Ser Leu Phe Gly Leu Glu Ser Tyr Gln Trp 435 440 445 Asn Asp Ile Thr Phe Glu Ala Ser Ala Arg Leu Glu Lys Gln Lys Val 450 455 460 Thr Lys Asp Tyr Asp His Glu Lys Val Arg Asn Glu Phe Leu Ser Leu 465 470 475 480 Gly Tyr Ile Asp Ser Lys Arg Pro Glu Thr Ala His Ala Met Asp Asn 485 490 495 Tyr Tyr Thr Leu Thr Gln Ala His Lys Glu Thr Ala His Ser Phe Ala 500 505 510 Leu Gly Ala His Trp Ala Phe Lys Glu Asn His Lys Leu Ser Leu Thr 515 520 525 Ala Ser His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His 530 535 540 Gly Met His Leu Ala Thr Asn Ser Phe Glu Met Gly Asn Lys Gly Leu 545 550 555 560 Ser Lys Glu Lys Ser Asn Asn Leu Asp Leu Gly Leu Ser Tyr Glu Gly 565 570 575 Asp Lys Phe Ser Tyr Tyr Leu Ser Gly Phe Leu Tyr Asn Phe Asp Asn 580 585 590 Tyr Thr Tyr Leu Tyr Thr Leu Asn Ser Gly Arg Gly Pro Ala Ser Met 595 600 605 Lys Gln Asp Ser Asp Leu Arg Ile Asn Arg Tyr Met Gln Ala Pro Ala 610 615 620 Arg Phe Tyr Gly Val Glu Val Asn Leu Gly Tyr Gln Val Thr Pro Lys 625 630 635 640 His His Ile Ser Leu Phe Gly Asp Tyr Val Lys Gly Tyr Leu Lys Ala 645 650 655 His Asp Ile Arg Thr Ser Asp Lys Val Ser Tyr Met Asp Asn Pro Glu 660 665 670 Phe Thr Lys Ala Val Glu Lys Leu Met Gln Glu Asn Pro Lys Met Lys 675 680 685 Pro Trp Arg Ala Ala Ser Lys Val Arg Asn Glu Met Gly Ile Glu Arg 690 695 700 Gln Ile Arg Val Gln Glu Pro Val Tyr Glu Glu Gln Pro Lys Met Tyr 705 710 715 720 Thr Pro Arg Leu Pro Pro Ile Arg Val Gly Ala Arg Ile Lys Ser Asp 725 730 735 Phe Thr Glu Asn Leu Lys Gly Glu Leu Glu Tyr Tyr His Val Phe Thr 740 745 750 Gln His Arg Ile Ser Lys Phe Glu Asn Val Thr Gln Gly His Asn Met 755 760 765 Leu Asn Leu Gly Leu Thr Tyr Gln Asn Lys Leu Ala Lys Gly Glu Tyr 770 775 780 Glu Ile Phe Leu Lys Ala Asn Asn Leu Leu Asn Glu Glu Val Tyr Ala 785 790 795 800 His Glu Thr Phe Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Asn 805 810 815 Val Gly Phe Asn Tyr Lys Phe 820 19823PRTActinobacillus minor NM305 19Met Lys Lys Asn Ala Ile Thr Leu Ser Met Ile Ser Leu Phe Ser Val 1 5 10 15 Cys Ala Tyr Ala Glu Glu Gly Ala Glu Leu Glu Glu Ile Gln Val Glu 20 25 30 Ala Lys Val Ala Glu Ser Asn Leu Leu Gly Glu Arg Pro Asn Val Ser 35 40 45 Asp Lys Leu Ile Asp Gly Lys Val Phe Lys Gln Lys Ser Thr Thr Leu 50 55 60 Gly Asp Ala Leu Ser Ser Glu Leu Gly Ile His Ser Asn Gln Tyr Gly 65 70 75 80 Gly Gly Ala Ser Ala Pro Ile Ile Arg Gly Gln Glu Gly Lys Arg Ile 85 90 95 Lys Ile Leu Gln Asn Asn Ser Asp Val Val Asp Met Ser Thr Met Ser 100 105 110 Pro Asp His Ala Val Thr Val Asp Thr Thr Leu Ser Lys Gln Val Glu 115 120 125 Ile Val Arg Gly Pro Ser Thr Leu Leu Tyr Ser Ser Gly Asn Ala Ala 130 135 140 Gly Val Ile Asn Val Leu Asp Asn Lys Ile Pro Asn Phe Met Pro Gln 145 150 155 160 Asn Gly Leu Lys Gly Glu Val Gly Phe Arg Phe Asn Thr Asn Asn Asn 165 170 175 Glu Lys Leu Thr Thr Ala Gly Val Thr Phe Ala Ile His Pro Ser Ile 180 185 190 Ala Val His Leu Glu Gly Leu Ser Lys Gln Ala Gly Asn Tyr Lys Thr 195 200 205 Pro His Tyr Arg Tyr Gly Thr Tyr Ala Asp Lys Lys Ala Phe Asn Arg 210 215 220 Arg Glu Met Ser Tyr Gln Asn Leu Ser Tyr Val Pro Glu Ser Trp Ala 225 230 235 240 Lys Ser Gln Val Gly Thr Ala Gly Ile Ser Trp Val His Glu Lys Gly 245 250 255 Tyr Leu Gly Leu Ala Tyr Thr Glu Arg Gln Asp Lys Tyr Gly Leu Pro 260 265 270 Ala His Asn His Ile Tyr Glu Gly Cys Ala Val Arg Ile Ile Asp Glu 275 280 285 Ser Val Lys Gln Gln Tyr Pro Tyr Leu Phe Pro Tyr Pro Glu Leu Ala 290 295 300 Asp Asp Leu His Leu Phe Trp Ala Asn Pro Gly Val Asn Met Ala Asp 305 310 315 320 Cys His Ala His Gly Leu Ser Ala Thr Pro Tyr Val Asp Leu Lys Ser 325 330 335 Gln Arg Tyr Asp Phe Arg Gly Glu Ile Ile Glu Pro Phe Lys Tyr Val 340 345 350 Asp Lys Leu Arg Phe Asn Ala Ser His Val Asn Tyr Gln His Gly Glu 355 360 365 Ile Glu Gly Glu Lys Ala Ala Asn Leu Phe Lys Asn Lys Gly Leu Thr 370 375 380 Thr Arg Leu Glu Phe Val His Ser Pro Ile Gly Asn Leu Thr Gly Ile 385 390 395 400 Trp Gly Ile Gln Tyr Leu Glu Gln Lys Asn Ser Ala Leu Ser Pro Glu 405 410 415 Asp Ser Ala Thr His Lys His Arg Gly Val Gln Gln Leu Leu Asn Asn 420 425 430 Asn Lys Met Gln Asn Trp Ser Leu Phe Gly Leu Glu Ser Tyr Gln Trp 435 440 445 Asn Asp Ile Thr Phe Glu Ala Ser Ala Arg Leu Glu Lys Gln Lys Val 450 455 460 Thr Lys Asp Tyr Asp His Glu Lys Val Arg Asn Glu Phe Leu Ser Leu 465 470 475 480 Gly Tyr Ile Asp Ser Lys Arg Pro Glu Thr Ala His Ala Met Asp Asn 485 490 495 Tyr Tyr Thr Leu Thr Gln Ala Tyr Lys Glu Thr Ala Arg Ser Phe Ala 500 505 510 Leu Gly Ala His Trp Ala Phe Lys Glu Asn His Lys Leu Ser Leu Thr 515 520 525 Ala Ser His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His 530 535 540 Gly Met His Leu Ala Thr Asn Ser Phe Glu Met Gly Asn Lys Gly Leu 545 550 555 560 Ser Lys Glu Lys Ser Asn Asn Leu Asp Leu Gly Leu Ser Tyr Glu Gly 565 570 575 Asp Lys Phe Ser Tyr Tyr Leu Ser Gly Phe Leu Tyr Asn Phe Asp Asn 580 585 590 Tyr Thr Tyr Leu Tyr Thr Leu Asn Ser Gly Arg Gly Pro Ala Ser Met 595 600 605 Lys Gln Asp Ser Asp Leu Arg Ile Asn Arg Tyr Met Gln Ala Pro Ala 610 615 620 Arg Phe Tyr Gly Val Glu Val Asn Phe Gly Tyr Gln Val Thr Pro Lys 625 630 635 640 His His Ile Ser Leu Phe Gly Asp Tyr Val Lys Gly Tyr Leu Lys Ala 645 650 655 His Asp Ile Arg Thr Ser Asp Lys Val Ser Tyr Val Asp Asn Pro Glu 660 665 670 Phe Thr Lys Ala Val Glu Lys Leu Met Gln Glu Asn Pro Lys Met Lys 675 680 685 Pro Trp Arg Ala Ala Ser Lys Val Arg Asn Glu Met Gly Ile Glu Arg 690 695 700 Gln Ile Arg Val Gln Glu Pro Ala Tyr Glu Glu Gln Pro Lys Met Tyr 705

710 715 720 Thr Pro Arg Leu Pro Pro Ile Arg Val Gly Ala Arg Ile Lys Ser Asp 725 730 735 Phe Thr Glu Asn Leu Lys Gly Glu Leu Glu Tyr Tyr His Val Phe Thr 740 745 750 Gln His Arg Ile Ser Lys Phe Glu Asn Val Thr Gln Gly His Asn Met 755 760 765 Leu Asn Leu Gly Leu Thr Tyr Gln Asn Lys Leu Ala Lys Gly Glu Tyr 770 775 780 Glu Ile Phe Leu Lys Ala Asn Asn Leu Leu Asn Glu Glu Val Tyr Ala 785 790 795 800 His Glu Thr Phe Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Asn 805 810 815 Ile Gly Phe Asn Tyr Lys Phe 820 20782PRTActinobacillus pleuropneumoniae L20 20Met Leu Ile Ser Ala Gln Phe Ser Pro Leu Val Trp Ala Asn Asn Asn 1 5 10 15 Asp Val Ala Val Leu Asp Glu Val Ser Val Val Gly Ser Thr Pro Ser 20 25 30 Ile Ser Gln Gly Ser Glu Val Thr Leu Leu Lys Val Ser Asp Lys Ile 35 40 45 Ile Ala Gly Lys Glu Phe Lys Lys Arg Ser Ala Thr Leu Gly Asn Ala 50 55 60 Leu Ala Ala Glu Leu Gly Val His Ser Asn Pro Phe Gly Gly Gly Ala 65 70 75 80 Ser Lys Pro Ile Ile Arg Gly Gln Glu Gly Ala Arg Ile Arg Ile Leu 85 90 95 Gln Asn Gly Ser Asp Val Ile Asp Met Ser Asn Leu Ser Pro Asp His 100 105 110 Ala Val Val Ala Asp Ser Leu Leu Ala Lys Gln Val Glu Ile Leu Arg 115 120 125 Gly Ser Ser Thr Leu Leu Tyr Ala Ser Ser Ser Pro Ala Gly Ile Val 130 135 140 Asn Val Val Asp Lys Arg Ile Pro Thr Glu Ile Pro Glu Lys Gly Tyr 145 150 155 160 Glu Val Glu Leu Asn Ser Arg Phe Asp Thr Ala Ala Lys Glu Lys Val 165 170 175 Gly Ala Leu Gly Ala Thr Phe Gly Ile Gly Lys His Ile Ala Val Arg 180 185 190 Ala Glu Gly Leu Thr Arg His Ser Asp Asn Tyr Arg Val Pro Gly Ile 195 200 205 Asn Leu Gly Glu Arg Leu Asn Tyr Val Pro Asp Thr Tyr Asn Lys Ser 210 215 220 Lys Val Gly Thr Leu Gly Leu Ser Phe Val Gly Glu Gln Gly Tyr Ile 225 230 235 240 Gly Ala Ser Tyr Ser Lys Arg Arg Asp Asn Tyr Gly Leu Pro Gly His 245 250 255 Asn His Lys Phe Asp Phe Cys Ile Gly His Ile Tyr Gly Asn Lys Gln 260 265 270 Gly Lys Tyr Ala Tyr Thr Tyr Leu Tyr Pro His Leu Ile Gly Glu Glu 275 280 285 Asn Ile Gly Ser Asn Pro His Phe His Cys Gly Thr Asp His Ala Glu 290 295 300 Asp Gly Thr His Ser His Asp Asn Pro Phe Glu His Asp His Asp His 305 310 315 320 Thr His Pro Gly Pro Trp Val Asp Leu Glu Ser Lys Arg Phe Asp Val 325 330 335 Lys Ala Glu Leu Arg Gln Pro Phe Lys Gly Ile Asp Lys Ile Lys Val 340 345 350 Ser Tyr Ala Asp Ala Asp Tyr Tyr His Asp Glu Lys Asp Ala Gly Val 355 360 365 Leu Ala Thr Arg Tyr His Lys Gln Leu Lys Lys Asp Gln Asp Tyr Gly 370 375 380 Lys Pro Val Asn Ile Phe Lys Asn Arg Gly Lys Asn Ala Arg Leu Glu 385 390 395 400 Ile Tyr His Ala Pro Leu Gly Gly Leu Thr Gly Val Trp Gly Val Gln 405 410 415 Tyr Gln Thr Gln Lys Ser Ser Met His Ala Pro Lys Asp Arg Glu Val 420 425 430 Lys Phe Pro Leu Val Glu Asn Thr Asn Lys Gln Met Ser Leu Phe Gly 435 440 445 Ile Glu Gln Tyr Met Trp Asp Asn Phe Ala Leu Glu Phe Ala Gly Arg 450 455 460 Val Glu Lys Gln Lys Ile Glu Ile Glu Tyr Asp Arg Asn Glu Ile Lys 465 470 475 480 Arg Leu Gln Asp His Tyr Arg Ile Ser Gly Gly Lys Gln Val Glu Pro 485 490 495 Asp Leu Ser Pro Tyr Asn Gln Asn Ala Tyr Ala Tyr Ser Ser Thr Leu 500 505 510 Asn Trp Phe Phe His Pro Asp Tyr Gln Leu Ser Phe Thr Ala Ser His 515 520 525 Asn Glu Arg Phe Pro Thr Pro Met Glu Leu Tyr Tyr His Gly Gln His 530 535 540 Ile Ala Thr Asn Ser Phe Glu Tyr Gly Asn Lys Asp Leu Lys Lys Glu 545 550 555 560 Gln Ser Asn Asn Val Glu Leu Gly Leu Gly Tyr Gln Thr Glu Arg Val 565 570 575 Gly Tyr Lys Val Asn Val Tyr Tyr Asn His Phe Lys Asn Tyr Ile Tyr 580 585 590 Asn Glu Asn Leu Phe Arg Glu Asn Gln Leu Phe Met Arg Arg Tyr Asn 595 600 605 Gln Ala Lys Ala Arg Phe Tyr Gly Ile Glu Ala Glu Ala Ser Tyr Arg 610 615 620 Phe Asn Asp Lys Tyr Gln Ala Thr Ile Phe Gly Asp Met Val Arg Gly 625 630 635 640 Trp Leu Thr Asn Leu Pro Pro Leu Thr Val Asn Ser Asp Tyr Ser Val 645 650 655 Phe Lys Asp Tyr Leu Pro Lys Asp Ala Lys Pro Gly Glu Asp Tyr Leu 660 665 670 Ile Tyr Arg Ala Asp Gln Asn Thr Pro Arg Thr Pro Pro Val Arg Leu 675 680 685 Gly Phe Arg Phe Asn Ala Glu Phe Thr Pro Asn Trp Ser Gly Asp Leu 690 695 700 Glu Leu Ile Arg Thr Phe Thr Gln Arg Arg Thr Ser Gln Leu Glu Tyr 705 710 715 720 Ile Thr Glu Gly Asn Thr Met Leu Asn Ile Gly Val Ala Tyr Ser Asn 725 730 735 Lys Trp Lys Asp Leu Asp Tyr Lys Ile Ser Leu Asn Gly Thr Asn Leu 740 745 750 Leu Asn Gln Pro Val Tyr Ile His Thr Ser Tyr His Gln Phe Val Pro 755 760 765 Gln Thr Gly Arg Asn Phe Ile Leu Val Val Asn Val Lys Phe 770 775 780 21782PRTActinobacillus pleuropneumoniae serovar 1 str. 4074 21Met Leu Ile Ser Ala Gln Phe Ser Pro Leu Val Trp Ala Asn Asn Asn 1 5 10 15 Asp Val Ala Val Leu Asp Glu Val Ser Val Val Gly Ser Thr Pro Ser 20 25 30 Ile Ser Gln Gly Ser Glu Val Thr Leu Leu Lys Val Ser Asp Lys Ile 35 40 45 Ile Ala Gly Lys Glu Phe Lys Lys Arg Ser Ala Thr Leu Gly Asn Ala 50 55 60 Leu Ala Ala Glu Leu Gly Val His Ser Asn Pro Phe Gly Gly Gly Ala 65 70 75 80 Ser Lys Pro Ile Ile Arg Gly Gln Glu Gly Ala Arg Ile Arg Ile Leu 85 90 95 Gln Asn Gly Ser Asp Val Ile Asp Met Ser Asn Leu Ser Pro Asp His 100 105 110 Ala Val Val Ala Asp Ser Leu Leu Ala Lys Gln Val Glu Ile Leu Arg 115 120 125 Gly Ser Ser Thr Leu Leu Tyr Ala Ser Ser Ser Pro Ala Gly Ile Val 130 135 140 Asn Val Val Asp Lys Arg Ile Pro Thr Glu Ile Pro Glu Lys Gly Tyr 145 150 155 160 Glu Val Glu Leu Asn Ser Arg Phe Asp Thr Ala Ala Lys Glu Lys Val 165 170 175 Gly Ala Leu Gly Ala Thr Phe Gly Ile Gly Lys His Ile Ala Val Arg 180 185 190 Ala Glu Gly Leu Thr Arg His Ser Asp Asn Tyr Arg Val Pro Gly Ile 195 200 205 Asn Leu Gly Glu Arg Leu Asn Tyr Val Pro Asp Thr Tyr Asn Lys Ser 210 215 220 Lys Val Gly Thr Leu Gly Leu Ser Phe Val Gly Glu Gln Gly Tyr Ile 225 230 235 240 Gly Ala Ser Tyr Ser Lys Arg Arg Asp Asn Tyr Gly Leu Pro Gly His 245 250 255 Asn His Lys Phe Asp Phe Cys Ile Gly His Ile Tyr Gly Asn Lys Gln 260 265 270 Gly Lys Tyr Ala Tyr Thr Tyr Leu Tyr Pro His Leu Ile Gly Glu Glu 275 280 285 Asn Ile Gly Ser Asn Pro His Phe His Cys Gly Thr Asp His Ala Glu 290 295 300 Asp Gly Thr His Ser His Asp Asn Pro Phe Gly His Asp His Asp His 305 310 315 320 Thr His Pro Gly Pro Trp Val Asp Leu Glu Ser Lys Arg Phe Asp Val 325 330 335 Lys Ala Glu Leu Arg Gln Pro Phe Lys Gly Ile Asp Lys Ile Lys Val 340 345 350 Ser Tyr Ala Asp Ala Asp Tyr Tyr His Asp Glu Lys Asp Ala Gly Val 355 360 365 Leu Ala Thr Arg Tyr His Lys Gln Leu Lys Lys Asp Gln Asp Tyr Gly 370 375 380 Lys Pro Val Asn Ile Phe Lys Asn Arg Gly Lys Asn Ala Arg Leu Glu 385 390 395 400 Ile Tyr His Ala Pro Leu Gly Gly Leu Thr Gly Val Trp Gly Val Gln 405 410 415 Tyr Gln Thr Gln Lys Ser Ser Met His Ala Pro Lys Asp Arg Glu Val 420 425 430 Lys Phe Pro Leu Val Glu Asn Thr Asn Lys Gln Met Ser Leu Phe Gly 435 440 445 Ile Glu Gln Tyr Met Trp Asp Asn Phe Ala Leu Glu Phe Ala Gly Arg 450 455 460 Val Glu Lys Gln Lys Ile Glu Ile Glu Tyr Asp Arg Asn Glu Ile Lys 465 470 475 480 Arg Leu Gln Asp His Tyr Arg Ile Ser Gly Gly Lys Gln Val Glu Pro 485 490 495 Asp Leu Ser Pro Tyr Asn Gln Asn Ala Tyr Ala Tyr Ser Ser Thr Leu 500 505 510 Asn Trp Phe Phe His Pro Asp Tyr Gln Leu Ser Phe Thr Ala Ser His 515 520 525 Asn Glu Arg Phe Pro Thr Pro Met Glu Leu Tyr Tyr His Gly Gln His 530 535 540 Ile Ala Thr Asn Ser Phe Glu Tyr Gly Asn Lys Asp Leu Lys Lys Glu 545 550 555 560 Gln Ser Asn Asn Val Glu Leu Gly Leu Gly Tyr Gln Thr Glu Arg Val 565 570 575 Gly Tyr Lys Val Asn Val Tyr Tyr Asn His Phe Lys Asn Tyr Ile Tyr 580 585 590 Asn Glu Asn Leu Phe Arg Glu Asn Gln Leu Phe Met Arg Arg Tyr Asn 595 600 605 Gln Ala Lys Ala Arg Phe Tyr Gly Ile Glu Ala Glu Ala Ser Tyr Arg 610 615 620 Phe Asn Asp Lys Tyr Gln Ala Thr Ile Phe Gly Asp Met Val Arg Gly 625 630 635 640 Trp Leu Thr Asn Leu Pro Pro Leu Thr Val Asn Ser Asp Tyr Ser Val 645 650 655 Phe Lys Asp Tyr Leu Pro Lys Asp Ala Lys Pro Gly Glu Asp Tyr Leu 660 665 670 Ile Tyr Arg Ala Asp Gln Asn Thr Pro Arg Thr Pro Pro Val Arg Leu 675 680 685 Gly Phe Arg Phe Asn Ala Glu Phe Thr Pro Asn Trp Ser Gly Asp Leu 690 695 700 Glu Leu Ile Arg Thr Phe Thr Gln Arg Arg Thr Ser Gln Leu Glu Tyr 705 710 715 720 Ile Thr Glu Gly Asn Thr Met Leu Asn Ile Gly Val Ala Tyr Ser Asn 725 730 735 Lys Trp Lys Asp Leu Asp Tyr Lys Ile Ser Leu Asn Gly Thr Asn Leu 740 745 750 Leu Asn Gln Pro Val Tyr Ile His Thr Ser Tyr His Gln Phe Val Pro 755 760 765 Gln Thr Gly Arg Asn Phe Ile Leu Val Val Asn Val Lys Phe 770 775 780 22790PRTActinobacillus pleuropneumoniae serovar 3 str. JL03 22Met Phe Asn Lys Lys Leu Leu Ala Val Leu Ile Ser Ala Gln Phe Ser 1 5 10 15 Pro Leu Val Trp Ala Asn Asn Asn Asp Val Ala Val Leu Asp Glu Val 20 25 30 Ser Val Val Gly Ser Thr Pro Ser Ile Ser Gln Gly Ser Glu Val Thr 35 40 45 Leu Leu Lys Val Ser Asp Lys Ile Ile Ala Gly Lys Glu Phe Lys Lys 50 55 60 Arg Ser Ala Thr Leu Gly Asn Ala Leu Ala Ala Glu Leu Gly Val His 65 70 75 80 Ser Asn Pro Phe Gly Gly Gly Ala Ser Lys Pro Ile Ile Arg Gly Gln 85 90 95 Glu Gly Ala Arg Ile Arg Ile Leu Gln Asn Gly Ser Asp Val Ile Asp 100 105 110 Met Ser Asn Leu Ser Pro Asp His Ala Val Val Ala Asp Ser Leu Leu 115 120 125 Ala Lys Gln Val Glu Ile Leu Arg Gly Ser Ser Thr Leu Leu Tyr Ala 130 135 140 Ser Ser Ser Pro Ala Gly Ile Val Asn Val Val Asp Lys Arg Ile Pro 145 150 155 160 Thr Glu Ile Pro Glu Lys Gly Tyr Glu Val Glu Leu Asn Ser Arg Phe 165 170 175 Asp Thr Ala Ala Lys Glu Lys Val Gly Ala Leu Gly Ala Thr Phe Gly 180 185 190 Ile Gly Lys His Ile Ala Val Arg Ala Glu Gly Leu Thr Arg His Ser 195 200 205 Asp Asn Tyr Arg Val Pro Gly Ile Asn Leu Gly Glu Arg Leu Asn Tyr 210 215 220 Val Pro Asp Thr Tyr Asn Lys Ser Lys Val Gly Thr Leu Gly Leu Ser 225 230 235 240 Phe Val Gly Glu Gln Gly Tyr Ile Gly Ala Ser Tyr Ser Lys Arg Arg 245 250 255 Asp Asn Tyr Gly Leu Pro Gly His Asn His Lys Phe Asp Phe Cys Ile 260 265 270 Gly His Ile Tyr Gly Asn Lys Gln Gly Lys Tyr Ala Tyr Thr Tyr Leu 275 280 285 Tyr Pro His Leu Ile Gly Glu Glu Asn Ile Gly Ser Asn Pro His Phe 290 295 300 His Cys Gly Thr Asp His Ala Glu Asp Gly Thr His Ser His Asp Asn 305 310 315 320 Pro Phe Gly His Asp His Asp His Thr His Pro Gly Pro Trp Val Asp 325 330 335 Leu Glu Ser Lys Arg Phe Asp Val Lys Ala Glu Leu Arg Gln Pro Phe 340 345 350 Lys Gly Ile Asp Lys Ile Lys Val Ser Tyr Ala Asp Ala Asp Tyr Tyr 355 360 365 His Asp Glu Lys Asp Ala Gly Val Leu Ala Thr Arg Tyr His Lys Gln 370 375 380 Leu Lys Lys Asp Gln Asp Tyr Gly Lys Pro Val Asn Ile Phe Lys Asn 385 390 395 400 Arg Gly Lys Asn Ala Arg Leu Glu Ile Tyr His Ala Pro Leu Gly Gly 405 410 415 Leu Thr Gly Val Trp Gly Val Gln Tyr Gln Thr Gln Lys Ser Ser Met 420 425 430 His Ala Pro Lys Asp Arg Glu Val Lys Phe Pro Leu Val Glu Asn Thr 435 440 445 Asn Lys Gln Met Ser Leu Phe Gly Ile Glu Gln Tyr Met Trp Asp Asn 450 455 460 Phe Ala Leu Glu Phe Ala Gly Arg Val Glu Lys Gln Lys Ile Glu Ile 465 470 475 480 Glu Tyr Asp Arg Asn Glu Ile Lys Arg Leu Gln Asp His Tyr Arg Ile 485 490 495 Ser Gly Gly Lys Gln Val Glu Pro Asp Leu Ser Pro Tyr Asn Gln Asn 500 505 510 Ala Tyr Ala Tyr Ser Ser Thr Leu Asn Trp Phe Phe His Pro Asp Tyr 515 520 525 Gln Leu Ser Phe Thr Ala Ser His Asn Glu Arg Phe Pro Thr Pro Met 530 535 540 Glu Leu Tyr Tyr His Gly Gln His Ile Ala Thr Asn Ser Phe Glu Tyr 545 550 555 560 Gly Asn Lys Asp Leu Lys Lys Glu Gln Ser Asn Asn Val Glu Leu Gly 565 570 575 Leu Gly Tyr Gln Thr Glu Arg Val Gly Tyr Lys Val Asn Val Tyr Tyr 580 585 590 Asn His Phe Lys Asn Tyr Ile Tyr Asn Glu Asn Leu Phe Arg Glu Asn 595 600 605

Gln Leu Phe Met Arg Arg Tyr Asn Gln Ala Lys Ala Arg Phe Tyr Gly 610 615 620 Ile Glu Ala Glu Ala Ser Tyr Arg Phe Asn Asp Lys Tyr Gln Ala Thr 625 630 635 640 Ile Phe Gly Asp Met Val Arg Gly Trp Leu Thr Asn Leu Pro Pro Leu 645 650 655 Thr Val Asn Ser Asp Tyr Ser Val Phe Lys Asp Tyr Leu Pro Lys Asp 660 665 670 Ala Lys Pro Gly Glu Asp Tyr Leu Ile Tyr Arg Ala Asp Gln Asn Thr 675 680 685 Pro Arg Thr Pro Pro Val Arg Leu Gly Phe Arg Phe Asn Ala Glu Phe 690 695 700 Thr Pro Asn Trp Ser Gly Asp Leu Glu Leu Ile Arg Thr Phe Thr Gln 705 710 715 720 Arg Arg Thr Ser Gln Leu Glu Tyr Ile Thr Glu Gly Asn Thr Met Leu 725 730 735 Asn Ile Gly Val Ala Tyr Ser Asn Lys Trp Lys Asp Leu Asp Tyr Lys 740 745 750 Ile Ser Leu Asn Gly Thr Asn Leu Leu Asn Gln Pro Val Tyr Ile His 755 760 765 Thr Ser Tyr His Gln Phe Val Pro Gln Thr Gly Arg Asn Phe Ile Leu 770 775 780 Val Val Asn Val Lys Phe 785 790 23689PRTAlcanivorax borkumensis SK2 23Met Thr Phe Arg Arg Gln Thr Leu Ser Gly Cys Val Ala Val Ala Leu 1 5 10 15 Leu Ala Pro Thr Ala Val Leu Ala Glu Ala Glu Asn Asn Ser Ala Gly 20 25 30 Leu Glu Lys Ala His Glu Leu Lys Gln Val Asp Val Ser Ala Leu Pro 35 40 45 Leu Gly Ser Ser Ser Ser Ser Thr Pro Tyr Ser Val Met Asp Gln Gln 50 55 60 Thr Leu Thr Gln Lys Asn Lys Asp Thr Leu Gly Glu Thr Leu Lys Asn 65 70 75 80 Gln Pro Gly Val His Ser Asp Thr Phe Gly Gly Gly Ala Ser Arg Pro 85 90 95 Val Ile Arg Gly Gln Gly Gly Pro Arg Ile Gly Ile Leu Ser Asp Gly 100 105 110 Ser Gln Val Leu Asp Ala Ser Ser Ile Ser Pro Asp His Ala Ile Thr 115 120 125 Val Asp Pro Met Leu Ala Arg Gln Ile Glu Val Leu Arg Gly Pro Ser 130 135 140 Thr Leu Leu Tyr Gly Ser Gly Ala Ser Gly Gly Ile Val Asn Val Leu 145 150 155 160 Asp Asn Arg Ile Pro Thr Gln Arg Pro Leu Glu Glu Val Glu Gly Phe 165 170 175 Val Ala Ile Arg Ala Asn Ser Val Ala Asp Glu Asn Ala Thr Ala Ala 180 185 190 Gly Ile Thr Val Ser Ala Thr Asp His Leu Val Phe Arg Val Glu Gly 195 200 205 Ser Arg Arg Asp Ala Glu Asp Tyr Glu Val Asn Gly Phe Glu Glu Leu 210 215 220 Thr Val Pro Gly Thr Tyr Ala Glu Ser Asp Asn Ala Ser Ile Gly Ala 225 230 235 240 Ser Trp Ile Gly Asp Gln Gly Phe Phe Gly Met Ala Tyr Ser Tyr Arg 245 250 255 Arg Asp Asn Tyr Gly Leu Pro Gly His Ser His Glu Phe Glu Glu Cys 260 265 270 Glu Ala Asn Gly Thr Ser Leu Ser Cys Pro Ser Gly Gly His Asp His 275 280 285 Asp His Glu Gly Glu Ile Val Pro Tyr Val Asp Leu Ala Ser Arg Arg 290 295 300 Val Asp Ala Arg Gly Glu Tyr Arg Asp Pro Phe Ser Gly Ile Ala Ala 305 310 315 320 Ile Arg Phe Arg Gly Asn His Thr Asp Tyr Arg His His Glu Ile Glu 325 330 335 Glu Gly Ala Ile Gly Ser Thr Phe Thr Asn Lys Gly Gly Asp Ala Arg 340 345 350 Leu Glu Val Glu His Lys Pro Phe Gly Asn Trp Arg Gly Val Val Gly 355 360 365 Leu Gln Tyr Thr Asn Phe Asp Phe Ser Ser Leu Gly His Glu Ala Phe 370 375 380 Val Pro Lys Thr Glu Thr Glu Ser Ile Ala Ile Phe Ala Val Glu Glu 385 390 395 400 Tyr Val Leu Asn Glu Gln Trp Ser Phe Glu Leu Gly Ala Arg Gln Glu 405 410 415 His Leu Thr Gln Thr Pro Asp Gln Arg Asn Gly Gln Ala Leu Ala Lys 420 425 430 Val Thr Ala Asp Asn Ala Ser Phe Ser Gly Ala Ala Asn Trp Ala Phe 435 440 445 Val Pro Gly Tyr Glu Leu Val Leu Ser Val Gly His Ser Gly Arg Ala 450 455 460 Pro Ser Ala Gln Glu Leu Tyr Ala Gln Gly Val His Leu Ala Thr Asn 465 470 475 480 Thr Tyr Glu Cys Gly Leu Leu Ser Asp Cys Gly Gly Gly Gln Arg Asp 485 490 495 Leu Asp Asp Glu Val Ser Leu Asn Ala Asn Leu Asn Leu Arg Lys Thr 500 505 510 Arg Gly Asp Trp Leu Phe Asp Leu Gly Val Phe Glu Asn Arg Ile Asn 515 520 525 Asn Tyr Ile Tyr Ala Arg Thr Leu Asp Gln Gln Glu Asp Phe Arg Leu 530 535 540 Ile Lys Tyr Ser Gln Arg Asp Ala Thr Phe Thr Gly Ala Glu Ala Ser 545 550 555 560 Val Ser Tyr Tyr Gly Ile Asp Pro Val Gly Ile Thr Val Phe Gly Asp 565 570 575 Met Val Arg Ala Thr Phe Asp Asp Gly Gly Tyr Leu Pro Arg Ile Pro 580 585 590 Ala Lys Arg Leu Gly Thr Arg Leu Asn Ser Tyr Leu Gly Asn Phe Asp 595 600 605 Gly Glu Leu Glu Val Tyr Arg Ser Phe Val Gln Asp Gln Leu Ser Gly 610 615 620 Phe Glu Glu Arg Thr Ala Ala Tyr Asp Met Val Asn Ala Thr Leu Ser 625 630 635 640 Tyr Arg Leu Arg Gly Asn Gln Arg Tyr Thr Phe Tyr Val Arg Gly Asn 645 650 655 Asn Leu Leu Gly Glu Glu Val Phe Asn His Ser Ser Phe Leu Ala Ser 660 665 670 Thr Val Pro Glu Pro Gly Arg Asn Ile Thr Val Gly Thr Arg Ile Glu 675 680 685 Phe 24691PRTAzoarcus sp. BH72 24Met Leu Pro Arg Leu Ser Pro Leu Ser Leu Ala Leu Leu Cys Ala Leu 1 5 10 15 Gly Thr Ala His Ala Ala Ala Gln Ser Ala Ala Pro Asn Asp Pro Thr 20 25 30 Ala Ala Pro Pro Val Gln Leu Glu Ala Val Thr Val Ser Ala Ser Gly 35 40 45 Leu Asp Val Asp Ser Gly Ala Met Ser Thr Pro Ala Thr Val Leu Gly 50 55 60 Gly Asp Glu Leu Val Arg Arg Arg Ala Ala Thr Leu Gly Glu Thr Leu 65 70 75 80 Ala Thr Glu Pro Gly Ile His Ala Thr Glu Phe Gly Ala Gly Ala Ser 85 90 95 Arg Pro Val Ile Arg Gly Met Asp Gly Ala Arg Val Arg Leu Leu Ser 100 105 110 Asp Gly Ala Glu Ile Met Asp Ala Ser Thr Ile Ser Pro Asp His Ala 115 120 125 Val Ala Ala Glu Pro Leu Leu Ser Glu Arg Ile Glu Val Leu Arg Gly 130 135 140 Pro Ser Ala Leu Ala Tyr Gly Gly Gly Ala Val Gly Gly Val Val Asn 145 150 155 160 Val Leu Asp Arg Arg Ile Pro Thr Ala Ile Pro Glu Arg Gly Val Glu 165 170 175 Gly Ser Val Glu Leu Arg Gly Asn Thr Ala Ala Arg Glu Ala Ala Gly 180 185 190 Ala Phe Glu Val Thr Ala Gly Ala Gly Asn Ile Ala Ile His Ala Glu 195 200 205 Gly Leu Lys Arg Asp Ala Arg Asp Tyr Arg Val Gly Asp Gly Trp Ala 210 215 220 Gly Gly Arg Arg Val Asp Gly Ser Tyr Asn Glu Thr Glu Thr Gly Ser 225 230 235 240 Leu Gly Leu Ser Trp Ile Gly Glu Arg Gly Tyr Leu Gly Val Ala Trp 245 250 255 Thr Arg Gln Arg Asn Glu Tyr Gly Leu Pro Gly His Ala His Asp Leu 260 265 270 Glu Asp Cys His Thr His Gly Asn Ser Leu His Cys Gly Gly His Asp 275 280 285 Gly Asp Asp Glu Asp Asp His Asp His Ala Asp Glu Gly Gly Gly Val 290 295 300 Pro Tyr Val Lys Leu Asp Ser Glu Arg Trp Asp Leu Arg Gly Glu Tyr 305 310 315 320 Arg Glu Pro Phe Ala Gly Ile Ser Arg Leu Arg Val Arg Ala Ser His 325 330 335 Thr Arg Tyr Arg His Asp Glu Ile Glu Asp Gly Ala Val Ser Thr Arg 340 345 350 Phe Arg Asn Asn Ala Ser Glu Gly Arg Val Glu Leu Gln His Ala Pro 355 360 365 Leu Gly Gly Trp Arg Gly Val Phe Gly Leu Gln Thr Thr Arg Arg Asp 370 375 380 Phe Ser Ala Ile Gly Glu Glu Ala Tyr Val Pro Pro Thr Leu Thr Arg 385 390 395 400 Arg His Gly Ala Phe Leu Ile Glu Glu Tyr Pro Thr Gly Asp Trp Arg 405 410 415 Phe Glu Ala Gly Leu Arg His Glu Trp Gln Gln Val Glu Val Asp Ala 420 425 430 Asp Ala Arg Asp Arg Ser His Arg Gly Asn Ser Leu Ser Leu Gly Ala 435 440 445 Val Trp Asn Phe Ala Pro Asp Tyr Ala Leu Gly Leu Ser Leu Ala Arg 450 455 460 Ala Gln Arg Leu Pro Thr Ala Glu Glu Leu Tyr Ala Asp Gly Leu His 465 470 475 480 Met Ala Thr Arg Thr Ile Glu Arg Gly Asn Ala Asp Leu Lys Ala Glu 485 490 495 Thr Ser His Asn Ile Asp Leu Ser Leu Lys Lys Leu Ala Gly Ala Thr 500 505 510 Thr Phe Asn Leu Ser Val Phe His Asn Arg Val Asn Asp Phe Ile Tyr 515 520 525 Ala His Thr Leu Asp Ala Leu Glu Gly Met Gln Leu Ile Glu Tyr Ala 530 535 540 Gln Arg Asp Ala Ile Phe Thr Gly Val Glu Gly Gln Val Arg Gln Gln 545 550 555 560 Leu Asp Arg Val Phe Gly Leu Thr Leu Phe Gly Asp Tyr Val Arg Ala 565 570 575 Arg Leu Ala Gly Gly Asp Gly Asp Arg Asp Arg Asp Leu Pro Arg Ile 580 585 590 Pro Ala His Arg Val Gly Leu Arg Leu Asp Ala Arg Gln Gly Ala Trp 595 600 605 Gln Gly Glu Leu Glu Val Tyr Arg Val Gly Arg Gln Arg Gln Val Ala 610 615 620 Glu Phe Glu Ser Ser Thr Pro Gly Tyr Asn Met Val Asn Leu Gly Ala 625 630 635 640 Ser Tyr Ala Gly Arg Ile Ala Ser Val Pro Tyr Leu Phe Tyr Val Lys 645 650 655 Ala Ala Asn Leu Thr Asp Glu Leu Ala Tyr Ser His Thr Ser Phe Ile 660 665 670 Lys Asp Ala Ala Pro Leu Met Gly Arg Asn Leu Thr Met Gly Val Lys 675 680 685 Val Thr Phe 690 25682PRTAzotobacter vinelandii DJ 25Met Lys Arg His Ser Leu Ala Trp Thr Leu Gly Leu Ser Leu Val Pro 1 5 10 15 Ala Ile Gly Ala Ser Glu Pro Leu Glu Leu Glu Ser Thr Val Val Ser 20 25 30 Ala Ser Ala Leu Ala Lys Lys Pro His Glu Met Thr Thr Pro Ala Glu 35 40 45 Val Leu Glu Gly Asp Glu Leu Val Leu Arg Arg Glu Ala Thr Leu Gly 50 55 60 Glu Thr Leu Glu Gly Leu Pro Gly Val Arg Ser Ala Ser Phe Gly Ala 65 70 75 80 Gly Val Gly Arg Pro Met Ile Arg Gly Gln Gln Gly Ala Arg Val Lys 85 90 95 Met Leu Ser Asp Gly Val Asp Val Leu Asp Ala Ser Asn Ile Ser Pro 100 105 110 Asp His Ala Val Ser Ser Glu Pro Leu Met Ala Glu Arg Ile Glu Val 115 120 125 Leu Lys Gly Pro Ala Thr Leu Leu Tyr Gly Gly Gly Ala Ile Gly Gly 130 135 140 Val Val Asn Val Ile Asp Lys Lys Val Pro Thr His Val Pro Glu Asn 145 150 155 160 Gly Tyr Glu Gly Glu Leu Glu Leu Arg Ala Asn Ser Val Ala Asn Glu 165 170 175 Gly Ser Gly Val Phe Gly Ile Thr Ala Gly Ser Gly Asn Phe Ala Val 180 185 190 Arg Val Glu Gly Val Lys Arg Gln Ala Asp Asp Tyr Glu Ile His Gly 195 200 205 Ser Pro Ser Lys Gln Asp Gly Ser Tyr Asn Asp Thr Asp Thr Phe Ser 210 215 220 Leu Gly Ala Ser Phe Val Gly Glu Arg Gly Tyr Leu Gly Val Ala Phe 225 230 235 240 Thr Glu Gln Asn Asn Arg Tyr Gly Leu Leu Gly His Gln His Ala Asp 245 250 255 Cys His Leu Asp Gly Pro Ala Trp His Cys Gly Glu His Glu Asp Glu 260 265 270 His Glu Glu Glu His Glu Asp Glu Glu His Glu Asp His Asp Glu His 275 280 285 Asp His Glu His Glu Gly Glu Gly Val Pro Tyr Val Asp Leu Arg Gln 290 295 300 Tyr Arg Trp Asp Leu Arg Gly Glu Leu Gln Asp Pro Leu Pro Gly Phe 305 310 315 320 Glu Leu Ala Arg Leu Arg Val Ala His Ser Asp Tyr Arg His Glu Glu 325 330 335 Val Glu Gly Gly Gln Val Ser Thr Arg Phe Asp Asn Asp Ala Ser Asp 340 345 350 Ala Arg Leu Glu Leu Thr His Gln Pro Leu Phe Gly Trp Arg Gly Val 355 360 365 Leu Gly Gly Gln Thr Leu Arg Arg Asp Phe Arg Ala Ser Gly Glu Glu 370 375 380 Ala Tyr Val Pro Pro Thr Ile Thr Arg Asn His Ala Leu Phe Leu Leu 385 390 395 400 Glu Glu Tyr Thr Asp Gly Ala Trp Arg Tyr Glu Leu Gly Leu Arg His 405 410 415 Glu Trp Gln Asp Ile Glu Ala Glu Gly Arg Pro Asp Lys Asp His Arg 420 425 430 Gly Thr Ser Phe Ser Ala Gly Ala Val Trp Ser Phe Ala Pro Asn Tyr 435 440 445 Ser Leu Gly Phe Ser Leu Ser Arg Ser Gln Arg Leu Pro Ser Ala Glu 450 455 460 Glu Leu Tyr Ala Asp Gly Pro His Ala Ala Thr Arg Thr Val Glu Leu 465 470 475 480 Gly Asp Pro Glu Leu Asp Glu Glu Thr Ser His Asn Leu Glu Leu Thr 485 490 495 Leu Arg Lys Phe Ala Gly Arg Thr Thr Phe Ser Phe Thr Leu Tyr Arg 500 505 510 Asn Gln Val Asp Asp Tyr Ile Tyr Ala Ala Asp Thr Gly Arg Asp Ile 515 520 525 Gly Ser Gly Tyr Arg Glu Ile Glu Tyr Arg Gln Gln Asp Ala Leu Phe 530 535 540 Thr Gly Val Glu Gly Glu Val Arg Phe Gln Ala Thr Asp Ala Thr Ala 545 550 555 560 Leu Thr Leu Phe Gly Asp His Val Arg Gly Lys Leu Leu Asp Gly Gly 565 570 575 Gly Asp Leu Pro Arg Ile Pro Ala Gly Arg Leu Gly Val Arg Leu Asp 580 585 590 His Gly Phe Thr Ser Ser Leu Asp Gly Gln Leu Glu Phe Tyr Arg Val 595 600 605 Asn His Gln His Arg Leu Ala Asp Phe Glu Thr Glu Thr Ser Gly Tyr 610 615 620 Ser Met Leu Gly Ala Ser Leu Thr Tyr His Gly Ala Leu Arg Arg Ala 625 630 635 640 Asp Tyr Gln Ile Tyr Leu Lys Gly Asp Asn Leu Leu Asp Glu Lys Ala 645 650 655 Arg Asp His Ser Ser Phe Ile Lys Asp Asp Val Val Gln Pro Gly Arg 660 665 670 Asn Leu Thr Leu Gly Leu Arg Met Thr Phe 675 680 26713PRTBordetella bronchiseptica RB50 26Met Val Cys Tyr Ile Val Ser Phe Asn Glu Asn Gly Thr Ser Phe Tyr 1 5 10 15 Arg Glu Gly Asn Met Arg Phe Glu Arg His Pro Leu Ser Ala Ala Leu 20 25 30 Ala Leu Ala Leu Ala Trp Gln Gly Ala His Ala Gln Ala Ser Ala Asp 35 40

45 Gly Thr Pro Glu Ala Ala Thr Leu Ala Pro Ile Thr Val Ser Ala Ser 50 55 60 Pro Leu Ala Gly Asp Leu Asp Ser Met Thr Ala Pro Ala Ala Val Leu 65 70 75 80 Glu Gly Asp Gln Leu Leu Leu Arg Arg Gln Gly Thr Leu Gly Asp Thr 85 90 95 Leu Asp Gly Leu Pro Gly Val His Ala Asp Thr Phe Gly Gly Gly Ala 100 105 110 Ser Arg Pro Val Ile Arg Gly Gln Thr Ala Pro Arg Val Lys Val Leu 115 120 125 Ser Asp Gly Ser Glu Leu Met Asp Ala Ser Ala Ile Ser Pro Asp His 130 135 140 Ala Val Thr Thr Glu Pro Leu Leu Ala Asp Lys Ile Glu Val Leu Arg 145 150 155 160 Gly Pro Ala Thr Leu Leu Tyr Gly Gly Gly Ala Ile Gly Gly Val Val 165 170 175 Asn Val Leu Asp Arg Lys Ile Pro Thr Ala Val Pro Gln Gln Gly Ile 180 185 190 Glu Ala Glu Ala Glu Leu Arg Gly Ala Thr Gly Thr Lys Glu Arg Ala 195 200 205 Gly Ala Ile Gly Ile Thr Ala Gly Ser Gly Asn Phe Ala Val Arg Val 210 215 220 Glu Gly Leu Lys Arg Arg Ser Ser Asp Tyr Arg Val Pro Asp Trp Pro 225 230 235 240 Asp Gly Lys Leu Ala Gly Ser Tyr Ser Glu Ser Gly Gln Gly Thr Val 245 250 255 Gly Met Ser Trp Ile Thr Pro Arg Gly Tyr Val Gly Val Ala Phe Thr 260 265 270 His Leu Glu Ser Lys Tyr Gly Leu Pro Gly His Asn His Glu Tyr Glu 275 280 285 Gly Cys His Pro His Gly Ser His Leu His Cys Gly Gly His Asp Asp 290 295 300 His Gly His Gly His Asp Glu His Glu Glu Gly Glu Ala Glu His Asp 305 310 315 320 His Gly His Glu His Gly Ala Gly Asp Val Pro Tyr Val Lys Leu Arg 325 330 335 Ser Asn Arg Thr Asp Leu Arg Ala Glu Tyr Thr Asp Pro Phe Ala Gly 340 345 350 Phe Glu Lys Ile Arg Phe Arg Gly Gly Leu Thr Asp Tyr Arg His Asp 355 360 365 Glu Ile Glu Gly Gly Gln Leu Gly Thr Arg Phe Gln Asn Arg Gly Tyr 370 375 380 Asp Ala Arg Leu Glu Leu Thr His Arg Pro Leu Tyr Gly Trp His Gly 385 390 395 400 Val Val Gly Val Gln Thr Ser Tyr Ser Asp Phe Arg Ala Thr Gly Glu 405 410 415 Glu Ala Phe Leu Pro Arg Ser Lys Thr Arg Ala His Gly Leu Phe Leu 420 425 430 Leu Glu Glu Tyr Arg Trp Ala Asp Trp Arg Val Glu Leu Gly Ala Arg 435 440 445 Gln Asp Trp Gln Arg Val Ser Pro Gln Gly Gly Ala Pro Ala Ser Arg 450 455 460 Thr Ala Gly Thr Ser Leu Ser Ala Ala Ala Ile Trp Asp Phe Ala Pro 465 470 475 480 Gln Tyr Ser Leu Ala Leu Ser Val Ser Arg Ser Gln Arg Leu Pro Ser 485 490 495 Ala Gln Glu Leu Tyr Ala Asp Gly Val His Leu Ala Thr Asn Thr Tyr 500 505 510 Glu Ile Gly Asp Pro Gly Leu Asp Arg Glu Thr Ser Arg Asn Val Asp 515 520 525 Leu Thr Leu Arg Lys His Ser Gly Asp Thr Thr Phe Ser Val Ser Ala 530 535 540 Phe His Asn Arg Val Lys Asn Tyr Ile Tyr Ala Asn Thr Leu Asp Arg 545 550 555 560 Tyr Glu Asp Phe Arg Leu Ile Glu Tyr Thr Gln Arg Asp Ala Glu Phe 565 570 575 Thr Gly Val Glu Gly Glu Ile Arg His Arg Phe Gly Lys Val Phe Ser 580 585 590 Ala Ala Val Phe Gly Asp Tyr Val Arg Gly Arg Leu Thr Gly Gly Gly 595 600 605 Gly Asn Leu Pro Arg Ile Pro Ala Ala Arg Leu Gly Val Arg Ala Asp 610 615 620 Ala Gln Trp Gln Asn Trp Ala Gly Gly Val Glu Tyr Phe His Val Tyr 625 630 635 640 Arg Gln Asp Asp Ile Ala Ala Tyr Glu Ser Ser Thr Pro Gly Tyr Asp 645 650 655 Met Val Asn Ala Thr Ile Arg Tyr Arg Gly Lys Leu Asp Arg Thr Ala 660 665 670 Tyr Glu Ile Tyr Leu Arg Gly Asn Asn Leu Leu Asn Lys Leu Ala Phe 675 680 685 Asn His Ala Ser Phe Ile Ser Thr Val Ala Pro Leu Pro Gly Arg Ser 690 695 700 Val Leu Leu Gly Val Arg Leu Thr Tyr 705 710 27711PRTBordetella parapertussis 27Met Val Cys Tyr Ile Val Ser Phe Asn Glu Asn Gly Thr Ser Phe Cys 1 5 10 15 Arg Glu Gly Asn Met Arg Phe Glu Arg His Pro Leu Ser Ala Ala Leu 20 25 30 Ala Leu Ala Leu Ala Trp Gln Gly Ala His Ala Gln Ala Ser Ala Asp 35 40 45 Gly Thr Pro Glu Ala Ala Thr Leu Ala Pro Ile Thr Val Ser Ala Ser 50 55 60 Pro Leu Ala Gly Asp Leu Asp Ser Met Thr Ala Pro Ala Ala Val Leu 65 70 75 80 Glu Gly Asp Gln Leu Leu Leu Arg Arg Gln Gly Thr Leu Gly Asp Thr 85 90 95 Leu Asp Gly Leu Pro Gly Val His Ala Asp Thr Phe Gly Gly Gly Ala 100 105 110 Ser Arg Pro Val Ile Arg Gly Gln Thr Ala Pro Arg Val Lys Val Leu 115 120 125 Ser Asp Gly Ser Glu Leu Met Asp Ala Ser Ala Ile Ser Pro Asp His 130 135 140 Ala Val Thr Thr Glu Pro Leu Leu Ala Asp Lys Ile Glu Val Leu Arg 145 150 155 160 Gly Pro Ala Thr Leu Leu Tyr Gly Gly Gly Ala Ile Gly Gly Val Val 165 170 175 Asn Val Leu Asp Arg Lys Ile Pro Thr Ala Val Pro Gln Gln Gly Ile 180 185 190 Glu Ala Glu Ala Glu Leu Arg Gly Ala Thr Gly Thr Lys Glu Arg Ala 195 200 205 Gly Ala Ile Gly Ile Thr Ala Gly Ser Gly Asn Phe Ala Val Arg Val 210 215 220 Glu Gly Leu Lys Arg Arg Ser Ser Asp Tyr Arg Val Pro Asp Trp Pro 225 230 235 240 Asp Gly Lys Leu Ala Gly Ser Tyr Ser Glu Ser Gly Gln Gly Thr Val 245 250 255 Gly Met Ser Trp Ile Thr Pro Arg Gly Tyr Val Gly Val Ala Phe Thr 260 265 270 His Leu Glu Ser Lys Tyr Gly Leu Pro Gly His Asn His Glu Tyr Glu 275 280 285 Gly Cys His Pro His Gly Ser His Leu His Cys Gly Gly His Asp Asp 290 295 300 His Gly His Asp Glu His Glu Glu Gly Glu Ala Glu His Asp His Gly 305 310 315 320 His Glu His Gly Ala Gly Asp Val Pro Tyr Val Lys Leu Arg Ser Asn 325 330 335 Arg Thr Asp Leu Arg Ala Glu Tyr Thr Asp Pro Phe Ala Gly Phe Glu 340 345 350 Lys Ile Arg Phe Arg Gly Gly Leu Thr Asp Tyr Arg His Asp Glu Ile 355 360 365 Glu Gly Gly Gln Leu Gly Thr Arg Phe Gln Asn Arg Gly Tyr Asp Ala 370 375 380 Arg Leu Glu Leu Thr His Arg Pro Leu Tyr Gly Trp His Gly Val Val 385 390 395 400 Gly Val Gln Thr Ser Tyr Ser Asp Phe Arg Ala Thr Gly Glu Glu Ala 405 410 415 Phe Leu Pro Arg Ser Lys Thr Arg Ala His Gly Leu Phe Leu Leu Glu 420 425 430 Glu Tyr Arg Trp Ala Asp Trp Arg Val Glu Leu Gly Ala Arg Gln Asp 435 440 445 Trp Gln Arg Val Ser Pro Gln Gly Gly Ala Pro Ala Ser Arg Thr Ala 450 455 460 Gly Thr Ser Leu Ser Ala Ala Ala Ile Trp Asp Phe Ala Pro Gln Tyr 465 470 475 480 Ser Leu Ala Leu Ser Val Ser Arg Ser Gln Arg Leu Pro Ser Ala Gln 485 490 495 Glu Leu Tyr Ala Asp Gly Val His Leu Ala Thr Asn Thr Tyr Glu Ile 500 505 510 Gly Asp Pro Gly Leu Asp Arg Glu Thr Ser Arg Asn Val Asp Leu Thr 515 520 525 Leu Arg Lys His Ser Gly Asp Thr Thr Phe Ser Val Ser Ala Phe His 530 535 540 Asn Arg Val Lys Asn Tyr Ile Tyr Ala Asn Thr Leu Asp Arg Tyr Glu 545 550 555 560 Asp Phe Arg Leu Ile Glu Tyr Thr Gln Arg Asp Ala Glu Phe Thr Gly 565 570 575 Val Glu Gly Glu Ile Arg His Arg Phe Gly Lys Val Phe Ser Ala Ala 580 585 590 Val Phe Gly Asp Tyr Val Arg Gly Arg Leu Thr Gly Gly Gly Gly Asn 595 600 605 Leu Pro Arg Ile Pro Ala Ala Arg Leu Gly Val Arg Ala Asp Ala Gln 610 615 620 Trp Gln Asn Trp Ala Gly Gly Val Glu Tyr Phe His Val Tyr Arg Gln 625 630 635 640 Asp Asp Ile Ala Ala Tyr Glu Ser Ser Thr Pro Gly Tyr Asp Met Val 645 650 655 Asn Ala Thr Ile Arg Tyr Arg Gly Lys Leu Asp Arg Thr Ala Tyr Glu 660 665 670 Ile Tyr Leu Arg Gly Asn Asn Leu Leu Asn Lys Leu Ala Phe Asn His 675 680 685 Ala Ser Phe Ile Ser Thr Val Ala Pro Leu Pro Gly Arg Ser Val Leu 690 695 700 Leu Gly Val Arg Leu Thr Tyr 705 710 28713PRTBordetella pertussis Tohama I 28Met Ile Cys Tyr Ile Val Ser Phe Asn Glu Asn Gly Thr Ser Phe Tyr 1 5 10 15 Arg Glu Gly Asn Met Arg Phe Glu Arg His Pro Leu Ser Ala Ala Leu 20 25 30 Ala Leu Ala Leu Ala Trp Gln Gly Ala His Ala Gln Ala Ser Ala Asp 35 40 45 Gly Thr Ser Glu Ala Ala Thr Leu Ala Pro Ile Thr Val Ser Ala Ser 50 55 60 Pro Leu Ala Gly Asp Leu Asp Ser Met Thr Ala Pro Ala Ala Val Leu 65 70 75 80 Glu Gly Asp Gln Leu Leu Leu Arg Arg Gln Gly Thr Leu Gly Asp Thr 85 90 95 Leu Asp Gly Leu Pro Gly Val His Ala Asp Thr Phe Gly Gly Gly Ala 100 105 110 Ser Arg Pro Val Ile Arg Gly Gln Thr Ala Pro Arg Val Lys Val Leu 115 120 125 Ser Asp Gly Ser Glu Leu Met Asp Ala Ser Ala Ile Ser Pro Asp His 130 135 140 Ala Val Thr Thr Glu Pro Leu Leu Ala Asp Lys Ile Glu Val Leu Arg 145 150 155 160 Gly Pro Ala Thr Leu Leu Tyr Gly Gly Gly Ala Ile Gly Gly Val Val 165 170 175 Asn Val Leu Asp Arg Lys Ile Pro Thr Ala Val Pro Gln Gln Gly Ile 180 185 190 Glu Ala Glu Ala Glu Leu Arg Gly Ala Thr Gly Thr Lys Glu Arg Ala 195 200 205 Gly Ala Ile Gly Ile Thr Ala Gly Ser Gly Asn Phe Ala Val Arg Val 210 215 220 Glu Gly Leu Lys Arg Arg Ser Ser Asp Tyr Arg Val Pro Asp Trp Pro 225 230 235 240 Asp Gly Lys Leu Ala Gly Ser Tyr Ser Glu Ser Gly Gln Gly Thr Val 245 250 255 Gly Met Ser Trp Ile Thr Pro Arg Gly Tyr Val Gly Val Ala Phe Thr 260 265 270 His Leu Glu Ser Lys Tyr Gly Leu Pro Gly His Asn His Glu Tyr Glu 275 280 285 Gly Cys His Pro His Gly Ser His Leu His Cys Gly Gly His Asp Asp 290 295 300 His Gly His Gly His Asp Glu His Glu Glu Gly Glu Ala Glu His Asp 305 310 315 320 His Gly His Glu His Gly Ala Gly Asp Val Pro Tyr Val Lys Leu Arg 325 330 335 Ser Asn Arg Thr Asp Leu Arg Ala Glu Tyr Thr Asp Pro Phe Ala Gly 340 345 350 Phe Glu Lys Ile Arg Phe Arg Gly Gly Leu Thr Asp Tyr Arg His Asp 355 360 365 Glu Ile Glu Gly Gly Gln Leu Gly Thr Arg Phe Gln Asn Arg Gly Tyr 370 375 380 Asp Ala Arg Leu Glu Leu Thr His Arg Pro Leu Tyr Gly Trp His Gly 385 390 395 400 Val Val Gly Val Gln Thr Ser Tyr Ser Asp Phe Arg Ala Thr Gly Glu 405 410 415 Glu Ala Phe Leu Pro Arg Ser Lys Thr Arg Ala His Gly Leu Phe Leu 420 425 430 Leu Glu Glu Tyr Arg Trp Ala Asp Trp Arg Phe Glu Leu Gly Ala Arg 435 440 445 Gln Asp Trp Gln Arg Val Ser Pro Gln Ser Gly Ala Pro Ala Ser Arg 450 455 460 Thr Ala Gly Thr Ser Leu Ser Ala Ala Ala Ile Trp Asp Phe Ala Pro 465 470 475 480 Gln Tyr Ser Leu Ala Leu Ser Val Ser Arg Ser Gln Arg Leu Pro Ser 485 490 495 Ala Gln Glu Leu Tyr Ala Asp Gly Val His Leu Ala Thr Asn Thr Tyr 500 505 510 Glu Ile Gly Asp Pro Gly Leu Asp Arg Glu Thr Ser Arg Asn Val Asp 515 520 525 Leu Thr Leu Arg Lys His Ser Gly Asp Thr Thr Phe Ser Val Ser Ala 530 535 540 Phe His Asn Arg Val Lys Asn Tyr Ile Tyr Ala Asn Thr Leu Asp Arg 545 550 555 560 Tyr Glu Asp Phe Arg Leu Ile Glu Tyr Thr Gln Arg Asp Ala Glu Phe 565 570 575 Thr Gly Val Glu Gly Glu Val Arg His Arg Phe Gly Lys Val Phe Ser 580 585 590 Ala Ala Val Phe Gly Asp Tyr Val Arg Gly Arg Leu Thr Gly Gly Gly 595 600 605 Gly Asn Leu Pro Arg Ile Pro Ala Ala Arg Leu Gly Val Arg Ala Asp 610 615 620 Ala Gln Trp Gln Asn Trp Ala Gly Gly Val Glu Tyr Phe His Val Tyr 625 630 635 640 Arg Gln Asp Asp Ile Ala Ala Tyr Glu Ser Ser Thr Pro Gly Tyr Asp 645 650 655 Met Val Asn Ala Thr Ile Arg Tyr Arg Gly Lys Leu Asp Arg Thr Ala 660 665 670 Tyr Glu Ile Tyr Leu Arg Gly Asn Asn Leu Leu Asn Lys Leu Ala Phe 675 680 685 Asn His Ala Ser Phe Ile Ser Thr Val Ala Pro Leu Pro Gly Arg Ser 690 695 700 Val Leu Leu Gly Val Arg Leu Thr Tyr 705 710 29684PRTBordetella petrii 29Met Gln Ile Lys Pro Leu Pro Leu Ala Val Ala Met Ser Leu Gly Leu 1 5 10 15 Ala Ala Thr Ala Gln Ala Gln Gln Ala Gln Gln Ala Gln Gln Thr Gln 20 25 30 Gln Ala Pro Thr Leu Ala Pro Ile Thr Val Ser Ala Asn Pro Leu Gly 35 40 45 Leu Asp Pro Asp Ser Met Ala Leu Pro Ala Leu Val Leu Ser Gly Asp 50 55 60 Ala Leu Ile Glu Arg Arg His Asp Thr Leu Gly Asn Thr Leu Asp Gly 65 70 75 80 Leu Pro Gly Ile His Ser Asp Thr Phe Gly Gly Gly Ala Ser Arg Pro 85 90 95 Val Ile Arg Gly Gln Thr Ala Pro Arg Val Lys Val Leu Ser Asp Gly 100 105 110 Ser Glu Leu Met Asp Ala Ser Asn Ile Ser Pro Asp His Ala Val Thr 115 120 125 Thr Glu Pro Leu Leu Ala Asp Arg Ile Glu Val Leu Arg Gly Pro Ala 130 135 140 Thr Leu Leu Tyr Gly Gly Gly Ala Ile Gly Gly Val Val Asn Val Leu 145 150 155 160 Asp Arg Lys Ile Pro Thr Ala Val Pro Glu Lys Gly Val Glu Ala Glu 165 170 175 Ala Glu Val Arg Gly Ala Thr Gly Thr Gly Glu Arg Ala Gly Ala Val 180 185 190 Gly Ile Thr Ala Gly Thr Gly Glu Phe Ala Val Arg Val Glu Gly Leu 195 200

205 Lys Arg Arg Ser Asp Asp Tyr Asn Val Pro Asp Trp Pro Gly Gly Glu 210 215 220 Leu Glu Gly Ser Tyr Ser Arg Ser Ser Gln Gly Thr Val Gly Leu Ser 225 230 235 240 Trp Ile Thr Pro Arg Gly Tyr Val Gly Val Ala Tyr Thr Tyr Leu Glu 245 250 255 Ser Lys Tyr Gly Leu Pro Gly His Asn His Glu Tyr Glu Gly Cys His 260 265 270 Pro His Gly Thr His Leu His Cys Gly Gly His Asp His Asp His Asp 275 280 285 His Asp His Asp His Glu His Gly Glu Glu Asp Pro Pro Tyr Val Lys 290 295 300 Leu Arg Ser Asn Arg Val Asp Leu Arg Ala Glu Tyr Ala Glu Pro Phe 305 310 315 320 Ala Gly Phe Glu Lys Ile Arg Phe Arg Gly Gly Leu Thr Asp Tyr Gln 325 330 335 His Gln Glu Ile Glu Gly Gly Glu Val Gly Thr Thr Phe Lys Asn Arg 340 345 350 Gly Tyr Asp Ala Arg Leu Glu Leu Glu His Lys Pro Leu Tyr Gly Trp 355 360 365 Arg Gly Val Val Gly Ile Gln Asn Ala Tyr Ser Asp Phe Ser Ala Asp 370 375 380 Gly Glu Glu Ala Phe Leu Pro Arg Ser Asp Thr Arg Ser Thr Gly Ile 385 390 395 400 Phe Leu Leu Glu Glu Tyr Lys Leu Gly Asp Trp Arg Phe Glu Val Gly 405 410 415 Ala Arg Gln Asp Trp Gln Arg Ile Ser Pro Glu Gly Asp His Pro Arg 420 425 430 Ser Ser Gln Ser Gly Thr Ser Leu Ser Ala Ser Ala Ile Trp Asn Phe 435 440 445 Ala Pro Gln Tyr Ser Val Ala Leu Ser Leu Ser Arg Ser Gln Arg Leu 450 455 460 Pro Ser Ala Gln Glu Leu Tyr Ala Asp Gly Val His Leu Ala Thr Asn 465 470 475 480 Thr Tyr Glu Ile Gly Asn Pro Asp Leu Gly Lys Glu Thr Ser Arg Asn 485 490 495 Ile Asp Leu Thr Leu Arg Lys His Glu Gly Asp Thr Thr Phe Ser Leu 500 505 510 Ser Ala Phe His Asn Arg Val Lys Asn Tyr Ile Tyr Ala Asn Thr Leu 515 520 525 Asp Gln Phe Glu Asp Phe Arg Leu Ile Glu Tyr Thr Gln Arg Asp Ala 530 535 540 Glu Phe Thr Gly Leu Glu Gly Glu Val Arg His Gln Phe Thr Lys Val 545 550 555 560 Phe Ser Ala Ala Val Phe Gly Asp Tyr Val Arg Gly Lys Leu Thr Gly 565 570 575 Gly Gly Gly Asn Leu Pro Arg Ile Pro Ala Ala Arg Leu Gly Val Arg 580 585 590 Ala Asp Ala Lys Trp Arg Gln Trp Ser Gly Gly Val Glu Tyr Ala His 595 600 605 Val Phe Arg Gln Asn Asp Ile Ala Ser Tyr Glu Ser Ser Thr Pro Gly 610 615 620 Tyr Asp Met Val Asn Ala Val Val Ser Tyr Arg Gly Gly Leu Gly Ala 625 630 635 640 Asp Ala Ala Tyr Glu Val Tyr Leu Arg Gly Thr Asn Leu Leu Asp Lys 645 650 655 Leu Ala Tyr Asn His Ala Ser Phe Ile Ser Lys Val Ala Pro Leu Pro 660 665 670 Gly Arg Ser Val Leu Leu Gly Val Arg Val Thr Tyr 675 680 30711PRTComamonas testosteroni CNB-2 30Met Ser Gln Arg Phe Leu Asn Ser Ser Ala His Ser Leu His Arg Ser 1 5 10 15 Ser Lys Ser Ala Leu Ala Arg Ala Ile Glu Ala Leu Gln Pro Ala Gly 20 25 30 Arg Gly Leu Ala Leu Gly Thr Gly Leu Gly Leu Leu Ala Cys Ser Pro 35 40 45 Leu Val Phe Ala Gln Ser Asp Ala Ala Lys Gln Glu Ser Ser Leu Ala 50 55 60 Glu Val Thr Val Ser Ala Thr Gly Met Ala Ala Gly Asp Met Ala Thr 65 70 75 80 Pro Val Gln Val Leu Gly Glu Glu Glu Leu Arg Leu Arg Arg Ala Ala 85 90 95 Thr Leu Gly Glu Thr Leu Ala Ala Glu Pro Gly Ile Asn Ala Ser His 100 105 110 Phe Gly Ala Gly Ala Ser Arg Pro Val Ile Arg Gly Met Asp Gly Ala 115 120 125 Arg Val Ser Val Leu Ser Asp Gly Ser Glu Leu Leu Asp Ala Ser Thr 130 135 140 Val Ser Pro Asp His Ala Val Thr Thr Glu Pro Leu Leu Ala Asn Gln 145 150 155 160 Ile Glu Val Leu Arg Gly Pro Ser Ala Leu Leu Tyr Ser Pro Gly Ala 165 170 175 Met Gly Gly Val Val Asn Val Leu Asp Gly Lys Ile Pro Thr Arg Val 180 185 190 Pro Glu Lys Gly Leu Glu Gly Ser Ala Glu Val Gln Ala Gly Thr Ala 195 200 205 Ala Gly Met Ser Ala Gly Ala Phe Ser Leu Thr Thr Ala Thr Pro Leu 210 215 220 Lys Asn Asp Asn Gly Gln Leu Val Leu His Ala Glu Gly Val Ala Arg 225 230 235 240 Asn Ala Gly Asp Tyr Arg Val Gly Ser Gly Trp Gly Gln Ser Lys Val 245 250 255 Pro Gly Ser Phe Ser Arg Gly Asn Thr Gly Ser Val Gly Leu Ser Trp 260 265 270 Val Asp Asn Gln Gly Tyr Leu Gly Leu Ala Tyr Thr Arg Gln Gln Ala 275 280 285 Lys Tyr Gly Leu Pro Gly His Gln His Ser Phe Glu Gly Cys His Ala 290 295 300 His Gly Asp His Leu His Cys Gly Ser His Asp Gly His Asp His Asp 305 310 315 320 His Asp His Glu Glu Gly Gly Ser Val Pro Val Val Asp Leu Thr Ser 325 330 335 Glu Arg Trp Asp Leu Arg Gly Glu Trp Arg Lys Pro Thr Ala Gly Ile 340 345 350 Ala Ala Leu Arg Leu Arg Gly Gly Leu Thr Asn Tyr Arg His Asp Glu 355 360 365 Ile Glu Asp Gly Ser Val Ala Thr Gln Phe Arg Asn Lys Ala His Asp 370 375 380 Leu Arg Leu Glu Met Glu His Glu Pro Ile Ala Gly Trp Arg Gly Thr 385 390 395 400 Leu Gly Leu Gln Thr Leu Lys Arg Arg Phe Ser Ala Thr Gly Glu Glu 405 410 415 Ala Tyr Val Gln Pro Thr Asp Thr Gln Arg His Ser Leu Tyr Leu Leu 420 425 430 Glu Glu Tyr Arg Trp Gln Asp Trp Ser Phe Gln Gly Ala Leu Arg His 435 440 445 Asp Arg Gln Arg Val Glu Ala Glu Leu Ser Asn Glu Arg Arg Ser His 450 455 460 Ser Ala Thr Ser Ala Ser Leu Gly Thr Ala Trp Lys Phe Gln Pro Gly 465 470 475 480 Tyr Ser Ala Ser Ala Ser Phe Thr Ser Gly Ser Arg Met Pro Thr Ala 485 490 495 Glu Glu Leu Phe Ala Asn Gly Leu His Met Ala Thr Ala Thr Tyr Glu 500 505 510 Ile Gly Asn Pro Asn Leu Ser Arg Glu Arg Ser Gln Ala Leu Asp Leu 515 520 525 Gly Leu Ala Lys Thr Ala Gly Asp Thr Thr Trp Lys Leu Asn Ala Tyr 530 535 540 His Tyr Arg Ile Lys Gly Tyr Ile Tyr Gly Ala Thr Leu Asp Ala His 545 550 555 560 Glu Gly Leu Gln Leu Leu Gln Tyr Thr Gln Gly Asn Ala Arg Phe Thr 565 570 575 Gly Trp Glu Ala Gln Leu Ser Gln Arg Leu Ser Arg Glu Leu Ser Leu 580 585 590 Ser Val Phe Gly Asp Gly Val Arg Ala Arg Leu Glu Asp Gly Ser Ala 595 600 605 Leu Pro Arg Ile Pro Ala Leu Arg Ala Gly Leu Arg Val Asn Ala Arg 610 615 620 Leu Ala Gly Trp Asp Thr Met Ala Glu Trp Thr Gln Val Leu Arg Gln 625 630 635 640 Asn Arg Thr Ala Gln Tyr Glu Thr Gln Thr Pro Gly Tyr Gly Met Leu 645 650 655 Asn Leu Gly Ala Ser Tyr Leu Trp Lys Ser Gly Gly Asn Gln Trp Gln 660 665 670 Phe Tyr Val Lys Gly Gln Asn Leu Thr Asn Arg Leu Ala Tyr Ala Ala 675 680 685 Thr Ser Phe Ile Lys Ser Ala Ala Pro Leu Thr Gly Arg Asn Leu Val 690 695 700 Val Gly Leu Arg Met Asp Phe 705 710 31711PRTComamonas testosteroni KF-1 31Met Tyr Gln Arg Phe Ser Asn Ser Ser Ala His Ser Leu Arg Arg Ser 1 5 10 15 Pro Lys Ser Ala Leu Ala Arg Ala Ile Glu Ala Leu Gln Pro Ala Gly 20 25 30 Arg Gly Leu Ala Leu Gly Thr Gly Leu Gly Leu Leu Ala Cys Ser Pro 35 40 45 Leu Val Phe Ala Gln Ser Glu Ala Ala Lys Gln Glu Ser Ser Leu Ala 50 55 60 Glu Val Thr Val Ser Ala Thr Gly Met Ala Ala Gly Asp Met Ala Thr 65 70 75 80 Pro Val Gln Val Leu Gly Glu Glu Glu Leu Arg Leu Arg Arg Ala Ala 85 90 95 Thr Leu Gly Glu Thr Leu Ala Ala Glu Pro Gly Ile Asn Ala Ser His 100 105 110 Phe Gly Ala Gly Ala Ser Arg Pro Val Ile Arg Gly Met Asp Gly Ala 115 120 125 Arg Val Ser Val Leu Ser Asp Gly Ser Glu Leu Leu Asp Ala Ser Thr 130 135 140 Val Ser Pro Asp His Ala Val Thr Thr Glu Pro Leu Leu Ala Arg Gln 145 150 155 160 Ile Glu Val Leu Arg Gly Pro Ser Ala Leu Leu Tyr Ser Pro Gly Ala 165 170 175 Met Gly Gly Val Val Asn Val Leu Asp Gly Lys Ile Pro Thr Gln Ala 180 185 190 Pro Glu Lys Gly Leu Glu Gly Ser Ala Glu Val Gln Ala Gly Thr Ala 195 200 205 Ala Gly Met Ser Ala Gly Ala Phe Ser Leu Thr Thr Ala Thr Pro Leu 210 215 220 Lys Asn Asp Asn Gly Gln Leu Val Leu His Ala Glu Gly Val Ala Arg 225 230 235 240 Asn Ala Gly Asp Tyr Arg Val Gly Ser Gly Trp Gly Gln Ser Lys Val 245 250 255 Pro Gly Ser Phe Ser Arg Gly Asn Thr Gly Ser Val Gly Leu Ser Trp 260 265 270 Val Gly Asn Gln Gly Tyr Leu Gly Leu Ala Tyr Thr Arg Gln Gln Ala 275 280 285 Lys Tyr Gly Leu Pro Gly His Gln His Ser Phe Glu Gly Cys His Ala 290 295 300 His Gly Asp His Leu His Cys Gly Ser His Asp Gly His Asp His Asp 305 310 315 320 His Gly His Glu Glu Ser Gly Ser Val Pro Val Val Asp Leu Thr Ser 325 330 335 Glu Arg Trp Asp Leu Arg Gly Glu Trp Arg Lys Pro Thr Ala Gly Ile 340 345 350 Ala Ala Leu Arg Leu Arg Gly Gly Leu Thr Asn Tyr Arg His Asp Glu 355 360 365 Ile Glu Asp Gly Ser Val Ala Thr Gln Phe Arg Asn Lys Ala His Asp 370 375 380 Leu Arg Leu Glu Met Glu His Glu Pro Ile Ser Gly Trp Arg Gly Thr 385 390 395 400 Leu Gly Leu Gln Thr Leu Lys Arg Arg Phe Ser Ala Thr Gly Glu Glu 405 410 415 Ala Tyr Val Gln Pro Thr Asp Thr Gln Arg His Ser Leu Tyr Leu Leu 420 425 430 Glu Glu Tyr Arg Trp Gln Asp Trp Ser Phe Gln Gly Ala Leu Arg His 435 440 445 Asp Arg Gln Arg Val Glu Ala Glu Leu Ser Asn Glu Arg Arg Ser His 450 455 460 Ser Ala Thr Ser Ala Ser Leu Gly Thr Val Trp Lys Phe Gln Pro Gly 465 470 475 480 Tyr Ser Ala Ser Ala Ser Phe Thr Ser Gly Ser Arg Met Pro Thr Ala 485 490 495 Glu Glu Leu Phe Ala Asn Gly Leu His Met Ala Thr Ala Thr Tyr Glu 500 505 510 Ile Gly Asn Pro Asn Leu Ser Arg Glu Arg Ser Gln Ala Leu Asp Leu 515 520 525 Gly Leu Ala Lys Thr Thr Gly Asp Thr Thr Trp Lys Leu Asn Ala Tyr 530 535 540 His Tyr Arg Ile Asn Gly Tyr Ile Tyr Gly Ala Thr Leu Asp Ala His 545 550 555 560 Glu Gly Leu Gln Leu Leu Gln Tyr Thr Gln Gly Asn Ala Arg Phe Thr 565 570 575 Gly Trp Glu Ala Gln Leu Ser Gln Arg Leu Ser Arg Glu Leu Ser Leu 580 585 590 Ser Val Phe Gly Asp Gly Val Arg Ala Arg Leu Glu Asp Gly Ser Ala 595 600 605 Leu Pro Arg Ile Pro Ala Leu Arg Ala Gly Leu Arg Val Asn Ala Arg 610 615 620 Leu Ala Gly Trp Asp Thr Met Ala Glu Trp Thr Gln Val Leu Arg Gln 625 630 635 640 Asn Arg Thr Ala Gln Tyr Glu Thr Gln Thr Pro Gly Tyr Gly Met Leu 645 650 655 Asn Leu Gly Ala Ser Tyr Leu Trp Lys Ser Gly Gly Asn Gln Trp Gln 660 665 670 Phe Tyr Val Lys Gly Gln Asn Leu Thr Asn Arg Leu Ala Tyr Ala Ala 675 680 685 Thr Ser Phe Ile Lys Ser Ala Ala Pro Leu Thr Gly Arg Asn Leu Val 690 695 700 Val Gly Leu Arg Met Asp Phe 705 710 32730PRTDelftia acidovorans SPH-1 32Met Arg Val Thr Thr Lys Gln Tyr His Gln Asp Ala Lys Ala Gln Gly 1 5 10 15 Glu Arg Thr Lys Leu Arg Pro Leu Leu Gln Gln Leu Leu Leu Ala Gly 20 25 30 Val Leu Gly Pro Ala Thr Gly Gly Leu Ala Met Ala Ala Glu Pro Ala 35 40 45 Asp Pro Val Pro Ser Ala Gly Pro Ala Ala Ala Ala Asn Ala Gly Ala 50 55 60 Gly Ala Pro Arg Ser Asp Ala Pro Val Leu Asn Glu Val Thr Val Lys 65 70 75 80 Ala Ser Gly Leu Gly Leu Thr Ser Ala Asp Met Ser Thr Pro Ala Thr 85 90 95 Val Leu Glu Gly Asp Val Leu Thr Leu Arg Gln Ala Ala Thr Leu Gly 100 105 110 Glu Thr Leu Asp Gly Glu Pro Gly Ile His Ala Ser His Phe Gly Ala 115 120 125 Gly Ala Ser Arg Pro Ile Ile Arg Gly Met Asp Gly Pro Arg Val Gln 130 135 140 Ile Leu Ser Asp Gly Ser Glu Leu His Asp Ala Ser Thr Ile Ser Pro 145 150 155 160 Asp His Ala Val Ala Ser Glu Pro Met Leu Ala Thr Gln Ile Glu Val 165 170 175 Leu Arg Gly Pro Ser Ala Leu Ile Tyr Gly Gly Gly Gly Ala Met Gly 180 185 190 Gly Val Val Asn Ile Leu Asp Asp Lys Ile Pro Thr Ser Ile Pro Arg 195 200 205 Asn Gly Ile Thr Gly Ser Ala Gln Leu Arg Gly Ser Thr Gly Ala Gly 210 215 220 Glu Thr Ala Gly Ala Phe Ser Leu Thr Gly Gly Ala Gly Gln Leu Ala 225 230 235 240 Ile His Ala Glu Gly Met Ala Arg Asp Ala Gly Asp Tyr Arg Val Gly 245 250 255 Ser Gly Trp Ala Pro Asn Gly Gln Ser Gln Gly Arg Val Ala Gly Ser 260 265 270 Phe Asn Arg Thr Asp Thr Gly Ser Leu Gly Leu Ser Trp Ile Gly Thr 275 280 285 Gln Gly Tyr Leu Gly Leu Ala Tyr Thr Arg Gln Thr Ala Arg Tyr Gly 290 295 300 Leu Pro Gly His Asn His Ser Phe Glu Gly Cys His Thr His Gly Asp 305 310 315 320 His Leu His Cys Gly Gly His Asp His Gly Asp Glu Asp Gly His Asp 325 330 335 His Gly Gly Asp Asp His Asp His Gly Ser Val Pro Val Val Asp Leu 340 345 350 Thr Ser Glu Arg Trp Asp Leu Arg Gly Glu Trp Arg Asn Pro Thr Ala 355 360 365 Gly Ile Ala Ala Leu Arg Val Arg Gly Gly Trp Thr Asp Tyr Arg His 370 375 380 Asp Glu Ile Glu Gly Gly Gln Val Ser Thr Thr Phe

Lys Asn Arg Ala 385 390 395 400 His Asp Leu Arg Val Glu Ala Asp Leu Glu Pro Ile Ala Gly Trp Arg 405 410 415 Gly Val Ile Gly Leu Gln Thr Val Gln Arg Lys Phe Ser Ala Glu Gly 420 425 430 Glu Glu Ala Tyr Val Gln Pro Thr Glu Thr Arg Arg Asn Ser Ile Tyr 435 440 445 Leu Leu Glu Glu Tyr Arg Trp Gln Asp Trp Arg Trp Gln Ala Ala Leu 450 455 460 Arg His Glu Arg Gln Thr Val Asp Ala Gln Gln Ser Gly Ile Ser Arg 465 470 475 480 Ser His Ser Gly Thr Ser Phe Ser Leu Gly Ser Val Trp Lys Phe Thr 485 490 495 Pro Gly Tyr Ser Leu Ser Ala Ser Leu Thr Gln Gly Asn Arg Leu Pro 500 505 510 Thr Ala Glu Glu Leu Tyr Ala Asn Gly Leu His Met Ala Thr Ser Thr 515 520 525 Tyr Glu Arg Gly Asn Ala Asp Leu Lys Arg Glu Arg Ser Gln Ala Leu 530 535 540 Asp Ile Gly Leu Arg Lys Thr Ala Gly Asp Thr Thr Phe Ser Val Asn 545 550 555 560 Ala Phe His His Arg Val Lys Gly Tyr Ile Tyr Gly Ala Thr Leu Asp 565 570 575 Glu His Asp Gly Leu Gln Leu Leu Gln Tyr Thr Gln Ala Asp Ala Arg 580 585 590 Phe Thr Gly Val Glu Gly Gln Val Arg Gln Lys Leu Ser Arg Gln Trp 595 600 605 Gly Val Thr Leu Phe Gly Asp Ala Val Arg Ala Arg Leu Glu Asp Gly 610 615 620 Ser Ala Leu Pro Arg Ile Pro Ala Ala Arg Val Gly Leu Arg Val Asp 625 630 635 640 Thr Phe Tyr Lys Gly Trp Asp Ala Gln Ala Glu Trp Val Gln Val Leu 645 650 655 Arg Gln Asn Arg Val Thr Ala Tyr Glu Thr Glu Thr Asp Gly Tyr Gly 660 665 670 Met Leu Asn Val Ser Ala Ser Tyr Thr Leu Arg Thr Ser Gly Gly Met 675 680 685 Pro Trp Gln Phe Phe Ile Lys Gly Asn Asn Leu Thr Asn Arg Leu Ala 690 695 700 Phe Ala His Thr Ser Phe Ile Lys Asn Ala Ala Pro Leu Met Gly Arg 705 710 715 720 Asn Ile Thr Val Gly Val Lys Val Ser Phe 725 730 33693PRTDiaphorobacter sp. TPSY 33Met Asn Ser Phe Thr Pro Phe Ala Arg Met Pro Gly Ala Met His Pro 1 5 10 15 Leu Ala Trp Ala Ala Leu Leu Cys Leu Thr Ser Ala Ala Pro Ala Trp 20 25 30 Ala Gln Ala Asp Ala Arg Leu Pro Glu Val Thr Val Ser Ser Ser Gly 35 40 45 Leu Gln Leu Gly Val Ser Glu Met Thr Gln Pro Val Ser Val Leu Glu 50 55 60 Gly Asp Ala Leu Val Arg Gln Arg Glu Ala Thr Leu Gly Glu Thr Leu 65 70 75 80 Asp Gly Glu Pro Gly Ile Thr Gly Ser His Phe Gly Ala Gly Ala Ser 85 90 95 Arg Pro Val Ile Arg Gly Met Asp Gly Pro Arg Val Arg Val Leu Ser 100 105 110 Asp Gly Ser Glu Leu His Asp Ala Ser Thr Val Ser Pro Asp His Ala 115 120 125 Val Ala Ala Glu Pro Leu Leu Ala Thr Gln Val Glu Val Leu Arg Gly 130 135 140 Pro Ser Ala Leu Val Tyr Gly Gly Gly Ala Val Gly Gly Val Val Asn 145 150 155 160 Val Leu Asp Gly Lys Val Pro Thr Ala Val Pro Asp Lys Gly Tyr Glu 165 170 175 Gly Ser Ala Glu Leu Arg Ala Gly Ser Ala Ala Arg Glu Lys Ala Gly 180 185 190 Ala Val Ala Leu Thr Gly Gly Ala Gly Asn Leu Ala Val His Val Glu 195 200 205 Ala Ala Gly Arg Asp Ala Asp Asp Tyr Arg Val Gly Ser Gly Trp Ala 210 215 220 Glu Gly Arg Arg Val Pro Gly Ser Phe Asn Arg Thr Gly Thr Gly Ser 225 230 235 240 Val Gly Leu Ser Trp Val Gly Ser Arg Gly Tyr Leu Gly Leu Ala Phe 245 250 255 Thr Arg Gln Asn Ala Lys Tyr Gly Leu Pro Gly His Ser His Ser Phe 260 265 270 Glu Gly Cys His Thr His Gly Asn His Leu His Cys Gly Ser His Asp 275 280 285 Glu His Asp His Glu Asp Asp Gly His Asp His Asp His Gly His Glu 290 295 300 Ala Val Pro Val Val Asp Leu Arg Ser Glu Arg Val Asp Ile Arg Gly 305 310 315 320 Glu Leu Arg Asp Pro Phe Thr Gly Phe Ser Ala Leu Arg Leu Arg Ala 325 330 335 Gly Val Thr Asp Tyr Val His Asp Glu Val Glu Glu Gly Thr Val Ala 340 345 350 Thr Thr Phe Lys Asn Lys Ala His Asp Leu Arg Val Glu Leu Gln His 355 360 365 Glu Pro Val Ala Gly Trp Arg Gly Val Leu Gly Leu Gln Thr Gly Gln 370 375 380 Arg Lys Phe Ser Ala Ala Gly Glu Glu Ala Tyr Val Gln Pro Thr Leu 385 390 395 400 Thr Arg Gln Trp Gly Val Phe Leu Leu Glu Glu Tyr Arg Leu Gly Asp 405 410 415 Trp His Gly Asp Trp Arg Ile Glu Thr Ala Leu Arg His Asp Arg Gln 420 425 430 Ser Ala Glu Ala Gln Asp Ser Gly Val Glu Arg Arg His His Gly Thr 435 440 445 Ser Ala Ser Leu Gly Ala Val Trp Arg Phe Ala Pro Gly Tyr Ala Ala 450 455 460 Gly Ala Ser Ile Thr Arg Ala His Arg Ala Pro Thr Ala Glu Glu Leu 465 470 475 480 Tyr Ala Arg Gly Leu His Met Ala Thr Ser Thr Tyr Glu Arg Gly Asp 485 490 495 Ala Ser Leu Arg Ala Glu Thr Ser Arg Asn Ile Asp Leu Ser Leu Arg 500 505 510 Lys Thr Ser Gly Asp Thr Thr Phe Asp Val Ser Val Phe His Asn Arg 515 520 525 Ile Arg Asn Tyr Ile Tyr Gly Arg Thr Leu Asp Glu Leu Asp Gly Leu 530 535 540 Gln Leu Leu Gln Tyr Ser Gln Ala Gly Ala Thr Phe Thr Gly Met Glu 545 550 555 560 Gly Arg Val Arg Gln Arg Ile Thr Gln Arg Leu Gly Val Thr Leu Phe 565 570 575 Gly Asp Ser Val Arg Ala Arg Leu Asp Gly Gly Ala Arg Leu Pro Arg 580 585 590 Ile Ala Pro Ala Arg Val Gly Leu Arg Leu Asp Ala Asn Trp Arg Asp 595 600 605 Trp Asp Gly Ala Val Glu Trp Val Gln Val Ala Arg Gln Asn Arg Val 610 615 620 Ala Ala Phe Glu Thr Ala Thr Pro Gly Tyr Gly Met Leu Asn Leu Gly 625 630 635 640 Leu Ala Tyr Asn Ser Arg Thr Gly Ser Gly Thr Pro Trp Gln Val Tyr 645 650 655 Leu Lys Ala Arg Asn Leu Thr Asp Arg Leu Ala Tyr Ala His Thr Ser 660 665 670 Phe Ile Lys Asp Ala Ala Pro Leu Ala Gly Arg Asn Val Thr Val Gly 675 680 685 Val Arg Val Ser Phe 690 34809PRTHaemophilus parasuis 29755 34Met Ile Asn Asn Arg Thr Thr Glu Gln Gln Asn Asn Arg Thr Thr Ala 1 5 10 15 Phe Ser Leu Ala Phe Ser Leu Leu Leu Cys Cys Leu Gly Ile Asn Ala 20 25 30 Glu Gln Leu Glu Leu Asp Glu Ile Ser Val Met Gly Lys Val Pro Glu 35 40 45 Gly Asn Ser Ile Ser Phe Leu Lys Val Ser Asp Ala Ile Ile Asp Gly 50 55 60 Glu Lys Phe Lys Asn Arg Ser Ala Thr Leu Gly Asn Ala Leu Ser Ser 65 70 75 80 Glu Leu Gly Val His Ser Thr Pro Phe Gly Gly Gly Ala Ser Ala Pro 85 90 95 Ile Ile Arg Gly Gln Glu Gly Val Arg Val Lys Ile Leu Gln Asn Asn 100 105 110 Ala Asp Val Val Asp Met Ser Asn Ile Ser Pro Asp His Ala Ile Thr 115 120 125 Ala Asp Thr Leu Leu Ala Asn Gln Val Glu Ile Leu Arg Gly Ala Ser 130 135 140 Thr Leu Leu Tyr Ala Ser Ser Ser Pro Ala Gly Ile Val Asn Ile Val 145 150 155 160 Asp Gln Arg Ile Pro Asn Lys Met Pro Lys Lys Gly Tyr Glu Val Thr 165 170 175 Leu Ser Ser Arg Phe Asp Thr Ala Ser Lys Glu Lys Val Tyr Ala Leu 180 185 190 Gly Thr Thr Ile Gly Ile Gly Lys His Leu Ala Leu Arg Leu Glu Gly 195 200 205 Leu Asp Arg Gln Ser Gln Asn Tyr Lys Val Pro Gln Ile Lys Leu Gly 210 215 220 Glu Thr Leu Asn Tyr Val Pro Asp Thr Tyr His Gln Ser Lys Val Gly 225 230 235 240 Thr Ile Gly Leu Ser Phe Ile Gly Glu Lys Gly Tyr Leu Gly Ala Ser 245 250 255 Tyr Asn Gln Arg Lys Asp Arg Tyr Gly Leu Pro Gly His Asn His Lys 260 265 270 Phe Asp Thr Cys Ile Ala His Ile Tyr Asp Met Arg Leu Gln Gly Lys 275 280 285 His Ser Tyr Thr Asn Leu Tyr Pro His Leu Met Ser Asp Glu Met Val 290 295 300 Thr Glu Asn Pro His Phe His Cys Gly Thr Asp Tyr Asp Leu Asp Pro 305 310 315 320 Ser His Ser His Asp His Pro Tyr Gly His Asp His Asp His Thr His 325 330 335 Ile Gly Pro Trp Val Asp Leu His Ser Lys Arg Ile Asp Ile Lys Gly 340 345 350 Glu Ile Lys Gln Pro Leu Pro Met Leu Asp Lys Ile Gln Leu Ser Tyr 355 360 365 Ala Gln Thr Asp Tyr Tyr His Asp Glu Lys Asp Ala Gly Lys Ser Gly 370 375 380 Asp Thr Ile Asn Pro Asn Arg Val Asp Lys Ser Lys Asp Phe Gly Lys 385 390 395 400 Pro Val Asn Ile Phe Lys Asn Gln Gly Lys Asn Ala Arg Leu Glu Phe 405 410 415 Phe His Thr Pro Ile Gly Gly Leu Thr Gly Met Phe Gly Val Gln Tyr 420 425 430 Gln Thr Leu Gln Ser Ser Ala Asn Thr Pro Asn Asn Arg Glu Val Gln 435 440 445 Trp Pro Leu Val Asp Asn Arg Asn Lys Gln Ile Ser Leu Phe Ala Leu 450 455 460 Glu Gln Tyr Ala Trp Asp Asn Phe Ala Ile Glu Leu Gly Leu Arg Thr 465 470 475 480 Glu Lys Gln Asn Ile His Ile Asp Tyr Asp Leu Ala Lys Ile Gln Lys 485 490 495 Gln Gln Lys Phe Asn Glu Arg Thr Tyr Gly Lys Gln Val Asp Pro Asp 500 505 510 Leu Ser Asp Tyr Asp Glu Lys Ala Ile Ser Tyr Thr Gly Ala Phe Asn 515 520 525 Trp Phe Phe His Pro Asp Tyr Gln Leu Ser Phe Thr Ala Ser His Asn 530 535 540 Glu Arg Leu Pro Thr Pro Met Glu Leu Tyr Tyr His Gly Gln His Leu 545 550 555 560 Ala Thr Asn Ser Phe Glu Tyr Gly Asn Lys Asp Leu Lys Lys Glu Ile 565 570 575 Ser Asn Asn Phe Glu Leu Gly Leu Gly Tyr His Thr Glu Lys Leu Asp 580 585 590 Tyr Lys Leu Ser Thr Tyr Tyr Asn Asn Phe Asp Asn Tyr Ile Tyr Asn 595 600 605 Glu Thr Leu Tyr Arg Ser Asn Asn Leu Phe Met Arg Arg Tyr Asn Gln 610 615 620 Ala Lys Ala Thr Phe Tyr Gly Leu Glu Gly Ile Ile Asn Tyr Arg Phe 625 630 635 640 Thr Pro Asp Tyr Gln Phe Ser Val Phe Gly Asp Met Val Lys Gly Lys 645 650 655 Leu Lys Gln Leu Pro Asp Ile Lys Gly Leu Asn Asp Val Tyr Gly Glu 660 665 670 Pro Ile Leu Asn Pro Asp Tyr Asp Pro Glu Tyr Asp Glu Pro Glu Asp 675 680 685 Gln Tyr Tyr Arg Pro Tyr Leu Gly Lys Glu Met Ile Lys Gln Ala Asp 690 695 700 Arg Val Ser Pro Arg Leu Pro Pro Ile Arg Leu Gly Ala Arg Phe Asn 705 710 715 720 Ala Gln Leu Thr Glu Asn Leu Ser Gly Ser Val Glu Trp Met Lys Val 725 730 735 Phe Thr Gln Asn Lys Val Ser Lys Leu Glu Ser Ser Thr Lys Gly Tyr 740 745 750 Gln Leu Leu Asn Ala Ser Leu Asn Tyr Arg Arg Gln Ile Lys Gly Val 755 760 765 Glu Tyr Thr Val Ser Leu Thr Gly Asn Asn Leu Leu Asn Gln Ala Val 770 775 780 Tyr Ile His Asn Ser Tyr His Pro Tyr Val Pro Gln Met Gly Arg Asn 785 790 795 800 Phe Ile Leu Gly Leu Asp Leu Ser Phe 805 35797PRTHaemophilus parasuis SH0165 35Met Ile Lys Asn Lys Thr Thr Ala Phe Ser Leu Leu Leu Cys Cys Leu 1 5 10 15 Gly Ile Asn Ala Glu Gln Leu Glu Leu Asp Glu Ile Ser Val Met Gly 20 25 30 Lys Val Pro Glu Gly Asn Ser Ile Ser Phe Leu Lys Val Ser Asp Ala 35 40 45 Ile Ile Asp Gly Glu Lys Phe Lys Asn Arg Ser Ala Thr Leu Gly Asn 50 55 60 Ala Leu Ser Ser Glu Leu Gly Val His Ser Thr Pro Phe Gly Gly Gly 65 70 75 80 Ala Ser Ala Pro Ile Ile Arg Gly Gln Glu Gly Val Arg Val Lys Ile 85 90 95 Leu Gln Asn Asn Ala Asp Val Val Asp Met Ser Asn Ile Ser Pro Asp 100 105 110 His Ala Ile Thr Ala Asp Thr Leu Leu Ala Asn Gln Val Glu Ile Leu 115 120 125 Arg Gly Ala Ser Thr Leu Leu Tyr Ala Ser Ser Ser Pro Ala Gly Ile 130 135 140 Val Asn Ile Val Asp Gln Arg Ile Pro Asn Lys Met Pro Lys Lys Gly 145 150 155 160 Tyr Glu Val Thr Leu Ser Ser Arg Phe Asp Thr Ala Ser Lys Glu Lys 165 170 175 Val Tyr Ala Leu Gly Thr Thr Ile Gly Ile Gly Lys His Leu Ala Leu 180 185 190 Arg Leu Glu Gly Leu Asp Arg Gln Ser Gln Asn Tyr Lys Val Pro Gln 195 200 205 Ile Lys Leu Gly Glu Thr Leu Asn Tyr Val Pro Asp Thr Tyr His Gln 210 215 220 Ser Lys Val Gly Thr Ile Gly Leu Ser Phe Ile Gly Glu Lys Gly Tyr 225 230 235 240 Leu Gly Ala Ser Tyr Asn Gln Arg Lys Asp Arg Tyr Gly Leu Pro Gly 245 250 255 His Asn His Lys Phe Asp Thr Cys Ile Ala His Ile Tyr Asp Met Arg 260 265 270 Leu Gln Gly Lys His Ser Tyr Thr Asn Leu Tyr Pro His Leu Met Ser 275 280 285 Asp Glu Met Val Thr Glu Asn Pro His Phe His Cys Gly Thr Asp Tyr 290 295 300 Asp Leu Asp Pro Ser His Ser His Asp His Pro Tyr Gly His Asp His 305 310 315 320 Asp His Thr His Ile Gly Pro Trp Val Asp Leu His Ser Lys Arg Ile 325 330 335 Asp Ile Lys Gly Glu Ile Lys Gln Pro Leu Pro Met Leu Asp Lys Ile 340 345 350 Gln Leu Ser Tyr Ala Gln Thr Asp Tyr Tyr His Asp Glu Lys Asp Ala 355 360 365 Gly Lys Ser Gly Asp Thr Ile Asn Pro Asn Arg Val Asp Lys Ser Lys 370 375 380 Asp Phe Gly Lys Pro Val Asn Ile Phe Lys Asn Gln Gly Lys Asn Ala 385 390 395 400 Arg Leu Glu Phe Phe His Thr Pro Ile Gly Gly Leu Thr Gly Met Phe 405 410 415 Gly Val Gln Tyr Gln Thr Leu Gln Ser Ser Ala Asn Thr Pro Ser Asn 420 425 430 Arg Glu Val Gln Trp Pro Leu Val Asp Asn Arg Asn Lys Gln Ile Ser 435 440 445 Leu Phe Ala Leu Glu Gln

Tyr Ala Trp Asp Asn Phe Ala Ile Glu Leu 450 455 460 Gly Leu Arg Thr Glu Lys Gln Asn Ile His Ile Asp Tyr Asp Leu Ala 465 470 475 480 Lys Ile Gln Lys Gln Gln Lys Phe Asn Glu Arg Thr Tyr Gly Lys Gln 485 490 495 Val Asp Pro Asp Leu Ser Asp Tyr Asp Glu Lys Ala Val Ser Tyr Thr 500 505 510 Gly Thr Phe Asn Trp Phe Phe His Pro Asp Tyr Gln Leu Ser Phe Thr 515 520 525 Ala Ser His Asn Glu Arg Leu Pro Thr Pro Met Glu Leu Tyr Tyr His 530 535 540 Gly Gln His Leu Ala Thr Asn Ser Phe Glu Tyr Gly Asn Lys Asp Leu 545 550 555 560 Lys Lys Glu Ile Ser Asn Asn Phe Glu Leu Gly Leu Gly Tyr His Thr 565 570 575 Glu Lys Leu Asp Tyr Lys Leu Ser Thr Tyr Tyr Asn Asn Phe Asp Asn 580 585 590 Tyr Ile Tyr Asn Glu Thr Leu Tyr Arg Ser Asn Asn Leu Phe Met Arg 595 600 605 Arg Tyr Asn Gln Ala Lys Ala Thr Phe Tyr Gly Leu Glu Gly Ile Ile 610 615 620 Asn Tyr Arg Phe Thr Pro Asp Tyr Gln Phe Ser Val Phe Gly Asp Met 625 630 635 640 Val Lys Gly Lys Leu Lys Gln Leu Pro Asp Ile Lys Gly Leu Asn Asp 645 650 655 Val Tyr Gly Glu Pro Ile Leu Asp Pro Asp Tyr Asp Pro Glu Tyr Asp 660 665 670 Glu Pro Glu Asp Gln Tyr Tyr Arg Pro Tyr Leu Gly Lys Glu Met Ile 675 680 685 Lys Gln Ala Asp Arg Val Ser Pro Arg Leu Pro Pro Ile Arg Leu Gly 690 695 700 Ala Arg Phe Asn Ala Gln Leu Thr Glu Asn Leu Ser Gly Ser Val Glu 705 710 715 720 Trp Met Lys Val Phe Thr Gln Asn Lys Val Ser Lys Leu Glu Ser Ser 725 730 735 Thr Lys Gly Tyr Gln Leu Leu Asn Ala Ser Leu Asn Tyr Arg Arg Gln 740 745 750 Ile Lys Gly Val Glu Tyr Thr Val Ser Leu Thr Gly Asn Asn Leu Leu 755 760 765 Asn Gln Ala Val Tyr Ile His Asn Ser Tyr His Pro Tyr Val Pro Gln 770 775 780 Met Gly Arg Asn Phe Ile Leu Gly Leu Asp Leu Ser Phe 785 790 795 36868PRTHaemophilus somnus 129PT 36Met Lys Leu Tyr Lys Phe Arg Lys Glu Leu Phe Val Leu Thr Phe Leu 1 5 10 15 Val Ser Gly Ser Ser Ile Ser Ala Glu Ile Ile Ala Glu Leu Asp Glu 20 25 30 Val Val Val Ser Asp Ser Ser Ser Val Ala Asp Phe Gln Ser Leu Ala 35 40 45 Phe Gln Gly Gly Arg Lys Ala Ser Asp Val Phe Ile Glu Gly Lys Glu 50 55 60 Phe Lys Thr Arg Ser Ala Thr Leu Gly Asn Ala Leu Ala Gly Glu Leu 65 70 75 80 Gly Val His Ser Asn Pro Phe Gly Gly Gly Ala Ser Ala Pro Val Ile 85 90 95 Arg Gly Gln Glu Gly Val Arg Ile Lys Leu Leu Gln Asn Gly Ser Asp 100 105 110 Ile Val Asp Met Ser Asn Ile Ser Pro Asp His Ala Val Val Ala Asp 115 120 125 Thr Leu Leu Ala Gln Gln Val Glu Leu Val Arg Gly Thr Ser Thr Leu 130 135 140 Met Tyr Gly Met Ala Ser Pro Ala Gly Val Val Asn Ile Val Asp Asn 145 150 155 160 Arg Ile Pro Thr Ser Arg Pro Glu Lys Gly Tyr Glu Gly Glu Ile Val 165 170 175 Ser Arg Phe Asp Thr Ala Ser Lys Glu Lys Val Leu Thr Ala Gly Val 180 185 190 Thr Leu Ala Ala Gly Asp Asn Phe Leu Ile Arg Ala Glu Gly Leu Thr 195 200 205 Arg Lys Ser Glu Asn Tyr His Val Pro Glu Val Phe Ile Gly Gln Lys 210 215 220 Leu Asn Tyr Leu Pro Asp Ser His His Lys Ser Lys Val Gly Thr Leu 225 230 235 240 Gly Thr Thr Trp Ile Gly Asp Lys Gly Tyr Leu Gly Ala Ser Val Ser 245 250 255 Tyr Arg Lys Asp Arg Tyr Gly Ile Pro Gly His Asn His Ala Phe Asp 260 265 270 Tyr Cys Ser Gly His Leu Phe Asp Thr Asp Asn Leu Lys Ala Ile Thr 275 280 285 Gly Gly Asp Gly Glu Ala Pro Tyr Leu Asn Ala Tyr Pro His Leu Met 290 295 300 Thr Asp Ala Asp Met Ile Ser Ser Leu His Phe His Cys Gly Ala Asp 305 310 315 320 Ile Gln Gly His Lys His Ser His Glu Ser Ile Tyr Gly His Asp His 325 330 335 Asp Ile Ser Glu Ala Gly Pro Val Val Asp Met Arg Ser Lys Arg Tyr 340 345 350 Asp Val Arg Gly Glu Trp Lys Ala Pro Leu Pro Trp Ile Ser Lys Val 355 360 365 Lys Leu Ser Leu Ala Tyr Ala Asp Tyr Tyr His Asp Glu Lys His Asp 370 375 380 Gly Lys Ala His Ile Asp Lys Asn Asp Ser Gln Gly Ile Lys Asp Arg 385 390 395 400 Lys Lys Tyr Thr Ala Ala Ile Met Ser Gly Lys Pro Glu Ala Phe Tyr 405 410 415 Ala Asn Arg Gly Phe Asn Ser Arg Leu Glu Ile Tyr His Gln Pro Thr 420 425 430 Glu His Phe Asn Gly Val Val Gly Met Gln Tyr Gln Thr Gln Lys Thr 435 440 445 Lys Val Gln Arg Leu Ala Pro Ser Leu Asn Asn Asn Gly Gln Asp Leu 450 455 460 Ser Gly Glu Arg Lys Glu Ser Glu Arg Asn Pro Leu Val Pro His Thr 465 470 475 480 Asn Lys Gln Phe Ser Val Phe Ala Leu Glu Gln Phe Thr Trp Arg Asn 485 490 495 Phe Ile Val Glu Val Gly Ala Arg Trp Glu Thr Gln Arg Ile Pro Ile 500 505 510 Lys Tyr Asp Pro Asn Lys Leu Arg Leu Asp Lys Ala Ala Gly Ser Lys 515 520 525 Val Arg Leu Pro Asp Leu Ser Pro Tyr Thr Glu Asn Ala Leu Ser Tyr 530 535 540 Ser Gly Thr Leu Met Trp Asp Phe His Pro Ala Tyr Arg Leu Ser Ile 545 550 555 560 Thr Gly Ser His Asn Glu Arg Ile Pro Ser Pro Met Glu Leu Tyr Tyr 565 570 575 His Gly Lys His Leu Ala Thr Asn Ser Phe Gln Tyr Gly Asn Lys Asp 580 585 590 Leu Lys Lys Glu Arg Ser Asn Asn Val Glu Ile Gly Leu Met Arg Ile 595 600 605 Ser Asp Lys Trp Asp Phe Lys Val Ser Ala Tyr Tyr Gln Arg Phe Lys 610 615 620 Asn Tyr Ile His Asn Glu Asn Leu Tyr Arg Glu Gly Asn Leu Phe Met 625 630 635 640 Arg Arg Tyr Asn Gln Ser Gln Ala Arg Phe Tyr Gly Phe Glu Gly Glu 645 650 655 Ile Gly Tyr Gln Ile Thr Pro Asn His Lys Ile Thr Phe Phe Gly Asp 660 665 670 Tyr Val Asn Gly Lys Leu Phe Gly Phe Lys Lys Phe Tyr Gly Asn Pro 675 680 685 Lys Phe Lys Lys Ile Cys Glu Ile Pro Lys Ala Gln Leu Ile Asp Ile 690 695 700 Glu Arg Cys Ile Glu Asp Glu Glu Trp Gly Glu Glu Trp Asp Tyr Arg 705 710 715 720 Lys Ile Gly Glu Gln Ile Ile Glu Arg Pro Asn Arg Asn Ala Ala Arg 725 730 735 Val Pro Pro Met Arg Leu Gly Phe Arg Leu Lys Ser Gln Phe Asn Asp 740 745 750 Asn Trp Ser Ser Ser Leu Glu Tyr Thr Arg Met Phe Ala Gln Lys Arg 755 760 765 Ile Ser Ile Asn Thr Val Ile Lys Glu Ile Asp Arg Glu Glu Ala Glu 770 775 780 Lys Arg Arg Glu Asn Ala Asn Gly Leu Gly Tyr Asp Gly His Tyr Thr 785 790 795 800 Glu Leu Val Pro Glu Asp Val Thr Gln Gly Tyr His Leu Leu Asn Leu 805 810 815 Ser Leu Asn Tyr Ser Arg Lys Ile Asp Gly Val Glu Tyr Ser Ala Thr 820 825 830 Leu Ser Ala Asn Asn Leu Leu Asn Gln Lys Ile Tyr Ile His Asn Ser 835 840 845 Tyr Leu Pro Tyr Val Pro Gln Met Gly Arg Asn Phe Ile Leu Asn Val 850 855 860 Gly Val Thr Phe 865 37868PRTHaemophilus somnus 2336 37Met Lys Leu Tyr Lys Phe Arg Lys Glu Leu Phe Val Leu Thr Leu Leu 1 5 10 15 Val Ser Gly Ser Ser Ile Ser Ala Glu Ile Ile Ala Glu Leu Asp Glu 20 25 30 Val Val Val Ser Asp Ser Ser Ser Val Ala Asp Phe Gln Ser Leu Ala 35 40 45 Phe Gln Gly Gly Arg Lys Ala Ser Asp Val Phe Ile Glu Gly Lys Glu 50 55 60 Phe Lys Thr Arg Ser Ala Thr Leu Gly Asn Ala Leu Ala Gly Glu Leu 65 70 75 80 Gly Val His Ser Asn Pro Phe Gly Gly Gly Ala Ser Ala Pro Val Ile 85 90 95 Arg Gly Gln Glu Gly Val Arg Ile Lys Leu Leu Gln Asn Gly Ser Asp 100 105 110 Ile Val Asp Met Ser Asn Ile Ser Pro Asp His Ala Val Val Ala Asp 115 120 125 Thr Leu Leu Ala Gln Gln Val Glu Leu Val Arg Gly Thr Ser Thr Leu 130 135 140 Met Tyr Gly Met Ala Ser Pro Ala Gly Val Val Asn Ile Val Asp Asn 145 150 155 160 Arg Ile Pro Thr Ser Arg Pro Glu Lys Gly Tyr Glu Gly Glu Ile Val 165 170 175 Ser Arg Phe Asp Thr Ala Ser Lys Glu Lys Val Leu Thr Ala Gly Val 180 185 190 Thr Leu Ala Ala Gly Asp Asn Phe Leu Ile Arg Ala Glu Gly Leu Thr 195 200 205 Arg Lys Ser Glu Asn Tyr His Val Pro Glu Val Phe Ile Gly Gln Lys 210 215 220 Leu Asn Tyr Leu Pro Asp Ser His His Lys Ser Lys Val Gly Thr Leu 225 230 235 240 Gly Thr Thr Trp Ile Gly Asp Lys Gly Tyr Leu Gly Ala Ser Val Ser 245 250 255 Tyr Arg Lys Asp His Tyr Gly Ile Pro Gly His Asn His Ala Phe Asp 260 265 270 Tyr Cys Ser Gly His Leu Phe Asp Thr Asp Asn Leu Lys Ala Ile Thr 275 280 285 Gly Gly Asp Gly Glu Ala Pro Tyr Leu Asn Ala Tyr Pro His Leu Met 290 295 300 Thr Asp Ala Asp Met Ile Ser Ser Leu His Phe His Cys Gly Ala Asp 305 310 315 320 Ile Gln Gly His Lys His Ser His Glu Ser Ile Tyr Gly His Asp His 325 330 335 Asp Ile Ser Glu Ala Gly Pro Val Val Asp Met Arg Ser Lys Arg Tyr 340 345 350 Asp Val Arg Gly Glu Trp Lys Ser Pro Ile Pro Gly Ile Ser Lys Val 355 360 365 Lys Leu Ser Leu Ala Tyr Ala Asp Tyr Tyr His Asp Glu Lys His Asp 370 375 380 Gly Lys Ala His Ile Asp Lys Asn Asp Ser Gln Gly Ile Lys Asp Arg 385 390 395 400 Lys Lys Tyr Thr Ala Ala Ile Met Ser Gly Lys Pro Glu Ala Phe Tyr 405 410 415 Ala Asn Arg Gly Phe Asn Ser Arg Leu Glu Ile Tyr His Gln Pro Thr 420 425 430 Glu His Phe Asn Gly Val Val Gly Met Gln Tyr Gln Thr Gln Lys Thr 435 440 445 Lys Val Gln Arg Leu Ala Pro Ser Leu Asn Asn Asn Gly Gln Asp Leu 450 455 460 Ser Gly Glu Arg Lys Glu Ser Glu Arg Asn Pro Leu Val Pro His Thr 465 470 475 480 Asn Lys Gln Phe Ser Val Phe Ala Leu Glu Gln Phe Thr Trp Arg Asn 485 490 495 Phe Ile Val Glu Val Gly Ala Arg Trp Glu Lys Gln Arg Ile Pro Ile 500 505 510 Lys Tyr Asp Pro His Lys Leu Arg Leu Asp Lys Ala Ala Gly Ser Lys 515 520 525 Val Arg Leu Pro Asp Leu Ser Pro Tyr Thr Glu Asn Ala Leu Ser Tyr 530 535 540 Ser Gly Thr Leu Met Trp Asp Phe His Pro Ala Tyr Arg Leu Ser Ile 545 550 555 560 Thr Gly Ser His Asn Glu Arg Ile Pro Ser Pro Met Glu Leu Tyr Tyr 565 570 575 His Gly Lys His Leu Ala Thr Asn Ser Phe Gln Tyr Gly Asn Lys Asp 580 585 590 Leu Lys Lys Glu Arg Ser Asn Asn Val Glu Ile Gly Leu Met Arg Ile 595 600 605 Ser Asp Lys Trp Asp Phe Lys Val Ser Ala Tyr Tyr Gln Arg Phe Lys 610 615 620 Asn Tyr Ile His Asn Glu Asn Leu Tyr Arg Glu Gly Asn Leu Phe Met 625 630 635 640 Arg Arg Tyr Asn Gln Ser Gln Ala Arg Phe Tyr Gly Phe Glu Gly Glu 645 650 655 Ile Gly Tyr Gln Ile Thr Pro Asn His Lys Ile Thr Phe Phe Gly Asp 660 665 670 Tyr Val Asn Gly Lys Leu Phe Gly Phe Lys Lys Phe Tyr Gly Asn Pro 675 680 685 Lys Phe Lys Arg Val Cys Glu Leu Glu Asn Asp Gly Lys Asn Asn Leu 690 695 700 Glu Lys Cys Val Glu Asn Glu Glu Trp Gly Glu Glu Trp Ser Tyr His 705 710 715 720 Lys Ile Gly Glu Gln Thr Ile Glu Arg Pro Asn Arg Asn Ala Ala Arg 725 730 735 Val Pro Pro Met Arg Leu Gly Phe Arg Leu Lys Ser Gln Phe Asn Asp 740 745 750 Asn Trp Ser Gly Ser Leu Glu Tyr Thr Arg Met Phe Val Gln Lys Arg 755 760 765 Ile Ser Ile Asn Ser Val Ile Lys Glu Ile Asp Arg Glu Glu Ala Glu 770 775 780 Lys Arg Arg Glu Asn Ala Lys Gly Leu Gly Tyr Asp Gly His Tyr Thr 785 790 795 800 Glu Leu Val Pro Glu Asp Val Thr Gln Gly Tyr His Leu Leu Asn Leu 805 810 815 Ser Leu Asn Tyr Ser Arg Lys Ile Asp Gly Val Glu Tyr Ser Ala Thr 820 825 830 Leu Ser Ala Asn Asn Leu Leu Asn Gln Lys Ile Tyr Ile His Asn Ser 835 840 845 Tyr Leu Pro Tyr Val Pro Gln Met Gly Arg Asn Phe Ile Leu Asn Val 850 855 860 Gly Val Thr Phe 865 38766PRTMannheimia haemolytica 38Met Leu Lys Lys Asn Tyr Leu Thr Val Ser Ile Leu Leu Ala Ile Ser 1 5 10 15 Gly Val Gly Tyr Ala Asn Glu Ile Ser Leu Glu Thr Ile Thr Val Asp 20 25 30 Gly Asn Thr Pro Ser Thr Lys Gly Lys Leu Leu Gly Gly Glu Leu Asn 35 40 45 Ser Asn Glu Ser Val Val Asp Glu Lys Asn Leu Lys Gln Gly Ser Ile 50 55 60 Thr Leu Gly Asn Ala Leu Ser Gly Glu Leu Gly Ile His Ser Ser Gln 65 70 75 80 Phe Gly Gly Gly Ala Ser Thr Pro Ile Ile Arg Gly Gln Glu Ser Lys 85 90 95 Arg Ala Lys Ile Leu Gln Asn Asn Gly Glu Asn Leu Asp Met Ser Gly 100 105 110 Met Ser Pro Asp His Ala Val Thr Val Asp Ala Leu Leu Ala Lys Arg 115 120 125 Ile Glu Ile Leu Arg Gly Pro Thr Thr Leu Leu Tyr Ser Ala Gly Asn 130 135 140 Thr Ala Gly Val Ile Asn Val Val Asp Asn Lys Ile Pro Thr Ala Ile 145 150 155 160 Pro Glu Lys Gly Tyr Glu Gly Gln Phe Gly Val Arg Phe Gly Ser Ala 165 170 175 Ser Lys Glu Arg Leu Thr Tyr Ala Gly Ser Thr Phe Ala Leu Gly Asn 180 185 190 His Leu Ala Leu Arg Val Gln Gly Met Tyr Asn Lys Ala Ser Glu Tyr 195 200 205 Tyr

Ala Pro His Phe Thr Ile Glu Gly Lys Pro Tyr His Arg Val Pro 210 215 220 Asp Ser Asp Val Gln Ser Gln Thr Gly Thr Val Ser Leu Ser Trp Ile 225 230 235 240 Gly Glu Arg Gly His Leu Gly Ile Ala Tyr Thr Asp Arg Arg Asp Lys 245 250 255 Tyr Gly Leu Ile Gly His Thr His Lys Tyr Asp His Tyr Thr Ile Ser 260 265 270 Ile Ile Arg Gln Ala Val Met Phe Ala Lys Gly Tyr Leu Arg Phe Tyr 275 280 285 Pro His Leu Ala Glu Glu Gly Asp Ile Asp Tyr Asn Asn Pro Gly Ile 290 295 300 Arg Leu Leu His Thr His Ile Pro Gly Gly Ser His Tyr Gly Gln Asp 305 310 315 320 Thr His Glu His Gly Lys Pro Trp Ile Asp Met His Ser Lys Arg Tyr 325 330 335 Asp Ile Asp Gly Ser Leu Gln Asn Pro Leu Pro Gly Phe Glu Glu Ala 340 345 350 Lys Ile Ser Ala Asn Tyr Val Asp Tyr Tyr His Asp Glu Lys Asp Gly 355 360 365 Lys Arg Val Glu Asn Tyr Phe Lys Asn Lys Gly Lys Asn Leu Arg Phe 370 375 380 Glu Leu Val His Lys Glu Trp Lys Gly Leu Lys Gly Ala Ile Gly Val 385 390 395 400 Gln Tyr Thr Asn Gln Ser Thr Ser Ala Leu Ala Leu Glu Ala Ser Arg 405 410 415 Ala Ala Lys Val Phe Asn Lys Gln Pro Leu Leu Asn Asn Pro Lys Thr 420 425 430 Lys Leu Trp Ser Leu Phe Ala Ile Glu Arg Leu Asn Leu Gly Asp Phe 435 440 445 Thr Phe Glu Leu Ser Gly Arg Ala Glu Arg Gln Lys Ile Ala Met Asp 450 455 460 Tyr Asp Val Lys Leu Ile Asp Arg Trp Leu Gly Phe Asn Thr Pro Met 465 470 475 480 Pro Asn Leu Asp Pro His Lys Asp Lys Gly Tyr Ser Tyr Ser Phe Ala 485 490 495 Thr His Trp Tyr Phe Ala Pro Asn His Lys Leu Thr Leu Asn Ala Ala 500 505 510 His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His Gly Lys 515 520 525 His Ile Ala Leu Asn Ala Phe Glu Ala Gly Asn Lys Asn Leu Lys Lys 530 535 540 Glu Arg Ser Asn Gln Ile Glu Leu Ser Leu Ala Tyr Val Gly Asp Lys 545 550 555 560 Trp Asp Tyr Lys Leu Asn Leu Tyr His Thr Arg Tyr Gly Asn Tyr Ile 565 570 575 Tyr Pro Leu Thr Leu Asn Asp Asn Arg Gly Pro Lys Ser Phe Thr Asp 580 585 590 Glu Tyr Asn Leu Lys Val Asn Arg Tyr Tyr Gln Gly Glu Ala Arg Phe 595 600 605 Ser Gly Ala Glu Gly Glu Ile Gly Tyr Leu Phe Thr Pro Asn Tyr Arg 610 615 620 Leu Ala Val Phe Gly Asp Tyr Val Arg Gly Lys Leu Val Asn Leu Pro 625 630 635 640 Asn Ile Ala Met Ser Tyr Asn Ile Trp Thr Gly Glu Val Asp Lys Trp 645 650 655 Ala Ser Gln Pro Asp Ile Ser Ala Pro Arg Ile Pro Pro Leu Arg Leu 660 665 670 Gly Ala Arg Phe Asn Ala Asp Phe Asn Leu Asn Trp Ser Gly Met Leu 675 680 685 Glu Tyr Tyr Arg Val Phe Ala Gln Lys Lys Val Ser Lys Tyr Glu Gln 690 695 700 Val Thr Pro Gly His His Gln Val Asn Leu Gly Val Thr Tyr Ser Asn 705 710 715 720 His Phe Asn Gln Thr Glu Tyr Gln Val Phe Leu Lys Val Asp Asn Leu 725 730 735 Leu Asn Gln Lys Met Tyr Gln His Ala Ser Tyr Leu Pro His Ile Pro 740 745 750 Gln Met Gly Arg Asn Ala Met Leu Gly Met Asn Ile Ser Phe 755 760 765 39790PRTMannheimia haemolytica PHL213 39Met Phe Lys Lys Thr Gly Leu Ala Leu Leu Ile Ser Ala Gln Phe Ser 1 5 10 15 Pro Leu Val Trp Ala Glu Asn Asn Asp Val Ala Val Leu Asp Glu Val 20 25 30 Ser Val Val Gly Ser Thr Pro Ser Ile Ser Gln Gly Ser Glu Val Thr 35 40 45 Leu Leu Lys Val Ser Asp Lys Ile Ile Ala Gly Lys Glu Phe Lys Lys 50 55 60 Arg Ser Ala Thr Leu Gly Asn Ala Leu Ala Ser Glu Leu Gly Val His 65 70 75 80 Ser Asn Pro Phe Gly Gly Gly Ala Ser Lys Pro Ile Ile Arg Gly Gln 85 90 95 Glu Gly Ala Arg Ile Arg Ile Leu Gln Asn Gly Ser Asp Val Ile Asp 100 105 110 Met Ser Asn Leu Ser Pro Asp His Ala Val Val Ala Asp Ser Leu Leu 115 120 125 Ala Asp Gln Val Glu Ile Leu Arg Gly Ser Ser Thr Leu Leu Tyr Ala 130 135 140 Ser Ser Ser Pro Val Gly Ile Val Asn Val Val Asp Lys Arg Ile Pro 145 150 155 160 Thr Ala Ile Pro Glu Lys Gly Tyr Glu Val Glu Leu Asn Ser Arg Phe 165 170 175 Asp Thr Ala Ala Lys Glu Lys Val Gly Ala Leu Gly Ala Thr Phe Gly 180 185 190 Ile Gly Asn His Ile Ala Val Arg Val Glu Gly Leu Ser Arg His Ser 195 200 205 Asp Asn Tyr Arg Val Pro Gly Ile Asn Leu Gly Glu Arg Leu Asn Tyr 210 215 220 Val Pro Asp Thr Tyr Asn Lys Ser Lys Val Gly Thr Leu Gly Leu Ser 225 230 235 240 Phe Val Gly Glu Arg Gly Tyr Ile Gly Ala Ser Tyr Ser Lys Arg Arg 245 250 255 Asp Asn Tyr Gly Leu Pro Gly His Asn His Lys Phe Asp Phe Cys Thr 260 265 270 Gly His Ile Tyr Gly Asn Lys Arg Asp Lys Tyr Ala Tyr Thr Tyr Leu 275 280 285 Tyr Pro His Leu Ile Gly Glu Glu Asn Ile Gly Ser Asn Pro His Phe 290 295 300 His Cys Gly Thr Asn His Ala Glu Asp Gly Thr His Ser His Asp Asn 305 310 315 320 Pro Phe Gly His Ala His Asp His Thr His Lys Gly Pro Trp Val Asp 325 330 335 Leu Glu Ser Lys Arg Ile Asp Val Lys Ala Glu Leu Arg Gln Pro Phe 340 345 350 Lys Gly Val Asp Lys Ile Lys Ala Ser Tyr Ala Asp Ala Asp Tyr Tyr 355 360 365 His Asp Glu Lys Asp Ala Gly Val Leu Ala Thr Arg Tyr His Lys Gln 370 375 380 Leu Lys Lys Asp Gln Asp Tyr Gly Lys Pro Val Asn Ile Phe Lys Asn 385 390 395 400 Arg Gly Lys Asn Thr Arg Leu Glu Val Tyr His Ala Pro Leu Gly Gly 405 410 415 Leu Thr Gly Val Trp Gly Val Gln Tyr Gln Thr Gln Lys Ser Ser Met 420 425 430 Asn Ala Pro Lys Asp Arg Glu Val Lys Phe Pro Leu Val Glu Asn Thr 435 440 445 Asn Lys Gln Phe Ser Leu Phe Gly Val Glu Gln Tyr Met Trp Asp Ser 450 455 460 Val Ala Val Glu Leu Ala Gly Arg Met Glu Lys Gln Lys Ile Glu Ile 465 470 475 480 Glu Tyr Asp Arg Asn Glu Ile Lys Arg Leu Gln Glu His Tyr Arg Ile 485 490 495 Ser Gly Gly Lys Gln Val Glu Pro Asp Leu Ser Pro Tyr Asp Glu Thr 500 505 510 Ala Tyr Ala Tyr Ser Gly Thr Leu Asn Trp Phe Phe His Pro Asp Tyr 515 520 525 Gln Leu Ser Phe Thr Ala Ser His Asn Glu Arg Leu Pro Thr Pro Met 530 535 540 Glu Leu Tyr Tyr His Gly Gln His Ile Ala Thr Asn Ser Phe Glu Tyr 545 550 555 560 Gly Asn Lys Asp Leu Lys Lys Glu Gln Ser Asn Asn Val Glu Leu Gly 565 570 575 Leu Gly Tyr Gln Ala Glu Arg Ile Gly Tyr Lys Val Ser Val Tyr Tyr 580 585 590 Asn His Phe Lys Asn Tyr Ile Tyr Asn Glu Asn Leu Phe Arg Glu Asn 595 600 605 Gln Leu Phe Met Arg Arg Tyr Asn Gln Ala Lys Ala Arg Phe Tyr Gly 610 615 620 Val Glu Ala Glu Ala Ser Tyr Arg Phe Asn Asp Gln Tyr Gln Ala Thr 625 630 635 640 Ile Phe Gly Asp Met Val Arg Gly Trp Leu Thr Asn Leu Pro Pro Leu 645 650 655 Lys Ile Asn Ser Asp Tyr Ser Val Phe Lys Asp Tyr Leu Pro Glu Asn 660 665 670 Val Glu Lys Gly Lys Asp Tyr Leu Leu Tyr Arg Ala Asp Gln Asn Thr 675 680 685 Pro Arg Thr Pro Pro Met Arg Leu Gly Phe Arg Phe Asn Ala Glu Phe 690 695 700 Thr Pro Asn Trp Ser Gly Asp Leu Glu Leu Ile Arg Thr Phe Thr Gln 705 710 715 720 Arg Arg Thr Ser Gln Leu Glu Tyr Ile Thr Glu Gly Asn Thr Met Leu 725 730 735 Asn Val Gly Leu Ser Tyr Ser Asn Lys Trp Lys Glu Leu Asp Tyr Lys 740 745 750 Ile Ser Leu Asn Gly Thr Asn Leu Leu Asn Gln Pro Val Tyr Ile His 755 760 765 Thr Ser Tyr His Gln Phe Val Pro Gln Met Gly Arg Asn Phe Met Leu 770 775 780 Gly Met Glu Met Lys Phe 785 790 40654PRTMannheimia haemolytica serotype A2 str. BOVINE 40Met Ser Pro Asp His Ala Val Thr Val Asp Ala Leu Leu Ala Lys Arg 1 5 10 15 Ile Glu Ile Leu Arg Asp Pro Thr Thr Leu Leu Tyr Ser Ala Gly Asn 20 25 30 Thr Ala Gly Val Ile Asn Val Val Asp Asn Lys Ile Pro Thr Ala Ile 35 40 45 Pro Glu Lys Gly Tyr Glu Gly Gln Phe Gly Val Arg Phe Gly Ser Ala 50 55 60 Ser Lys Glu Arg Leu Thr Tyr Ala Gly Ser Thr Phe Ala Leu Gly Asn 65 70 75 80 His Leu Ala Leu Arg Val Gln Gly Met Tyr Asn Lys Ala Ser Glu Tyr 85 90 95 Tyr Ala Pro His Phe Thr Ile Glu Gly Lys Pro Tyr His Arg Val Pro 100 105 110 Asp Ser Asp Val Gln Ser Gln Thr Gly Thr Val Gly Leu Ser Trp Ile 115 120 125 Gly Glu Arg Gly His Leu Gly Ile Ala Tyr Thr Asp Arg Arg Asp Lys 130 135 140 Tyr Gly Leu Ile Gly His Thr His Lys Tyr Asp His Tyr Thr Ile Ser 145 150 155 160 Ile Ile Arg Gln Ala Val Met Phe Ala Lys Gly Tyr Leu Arg Phe Tyr 165 170 175 Pro His Leu Ala Glu Glu Gly Asp Ile Asp Tyr Asn Asn Pro Gly Ile 180 185 190 Arg Leu Leu His Thr His Ile Pro Gly Gly Ser His Tyr Gly Gln Asp 195 200 205 Thr His Glu His Gly Lys Pro Trp Ile Asp Met His Ser Lys Arg Tyr 210 215 220 Asp Ile Asp Gly Ser Leu Gln Asn Pro Leu Pro Gly Phe Glu Glu Ala 225 230 235 240 Lys Ile Ser Ala Asn Tyr Val Asp Tyr Tyr His Asp Glu Lys Asp Gly 245 250 255 Lys Arg Val Glu Asn Tyr Phe Lys Asn Lys Gly Lys Asn Leu Arg Phe 260 265 270 Glu Leu Val His Lys Glu Trp Lys Gly Leu Lys Gly Ala Ile Gly Val 275 280 285 Gln Tyr Thr Asn Gln Ser Thr Ser Ala Leu Ala Leu Glu Ala Ser Arg 290 295 300 Ala Ala Lys Val Phe Asn Lys Gln Pro Leu Leu Asn Asn Pro Lys Thr 305 310 315 320 Lys Leu Trp Ser Leu Phe Ala Ile Glu Arg Leu Asn Leu Gly Asp Phe 325 330 335 Thr Phe Glu Leu Ser Gly Arg Ala Glu Arg Gln Lys Ile Ala Met Asp 340 345 350 Tyr Asp Val Lys Leu Ile Asp Arg Trp Leu Gly Phe Asn Thr Pro Met 355 360 365 Pro Asn Leu Asp Pro His Lys Asp Lys Gly Tyr Ser Tyr Ser Phe Ala 370 375 380 Thr His Trp Tyr Phe Ala Pro Asn His Lys Leu Thr Leu Asn Ala Ala 385 390 395 400 His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His Gly Lys 405 410 415 His Ile Ala Leu Asn Ala Phe Glu Ala Gly Asn Lys Asn Leu Lys Lys 420 425 430 Glu Arg Ser Asn Gln Ile Glu Leu Ser Leu Ala Tyr Val Gly Asp Lys 435 440 445 Trp Asp Tyr Lys Leu Asn Leu Tyr His Thr Arg Tyr Gly Asn Tyr Ile 450 455 460 Tyr Pro Leu Thr Leu Asn Asp Asn Arg Gly Pro Lys Ser Phe Thr Asp 465 470 475 480 Glu Tyr Asn Leu Lys Val Asn Arg Tyr Tyr Gln Gly Glu Ala Arg Phe 485 490 495 Ser Gly Ala Glu Gly Glu Ile Gly Tyr Leu Phe Thr Pro Asn Tyr Arg 500 505 510 Leu Ala Val Phe Gly Asp Tyr Val Arg Gly Lys Leu Val Asn Leu Pro 515 520 525 Asn Ile Ala Met Ser Tyr Asn Ile Trp Thr Gly Glu Val Asp Lys Trp 530 535 540 Ala Ser Gln Pro Asp Ile Ser Ala Pro Arg Ile Pro Pro Leu Arg Leu 545 550 555 560 Gly Ala Arg Phe Asn Ala Asp Phe Asn Leu Asn Trp Ser Gly Met Leu 565 570 575 Glu Tyr Tyr Arg Val Phe Ala Gln Lys Lys Val Ser Lys Tyr Glu Gln 580 585 590 Val Thr Pro Gly His His Gln Val Asn Leu Gly Val Thr Tyr Ser Asn 595 600 605 His Phe Asn Gln Thr Glu Tyr Gln Val Phe Leu Lys Val Asp Asn Leu 610 615 620 Leu Asn Gln Lys Met Tyr Gln His Ala Ser Tyr Leu Pro His Ile Pro 625 630 635 640 Gln Met Gly Arg Asn Ala Met Leu Gly Met Asn Ile Ser Phe 645 650 41793PRTMannheimia haemolytica serotype A2 str. BOVINE 41Met Ile Val Phe Asn Lys Lys Leu Leu Ala Leu Leu Ile Ser Ala Gln 1 5 10 15 Phe Ser Pro Leu Val Trp Ala Glu Ser Ser Asp Asp Val Ala Val Leu 20 25 30 Asn Glu Val Ser Val Val Gly Ser Thr Pro Ser Val Ala Lys Gly Ser 35 40 45 Glu Val Thr Leu Met Lys Thr Ser Asp Lys Ile Ile Glu Gly Lys Glu 50 55 60 Phe Lys Lys Arg Ser Ala Thr Leu Gly Asn Ala Leu Ala Ala Glu Leu 65 70 75 80 Gly Val His Ser Asn Pro Phe Gly Gly Gly Ala Ser Lys Pro Ile Ile 85 90 95 Arg Gly Gln Glu Gly Ala Arg Ile Arg Ile Leu Gln Asn Gly Ser Asp 100 105 110 Val Ile Asp Met Ser Asn Leu Ser Pro Asp His Ala Val Val Ala Asp 115 120 125 Ser Leu Leu Ala Asp Gln Val Glu Ile Leu Arg Gly Ser Ser Thr Leu 130 135 140 Leu Tyr Ala Ser Ser Ser Pro Val Gly Ile Val Asn Val Val Asp Lys 145 150 155 160 Arg Ile Pro Thr Ala Ile Pro Glu Lys Gly Tyr Glu Val Glu Leu Asn 165 170 175 Ser Arg Phe Asp Thr Ala Ala Lys Glu Lys Val Gly Ala Leu Gly Ala 180 185 190 Thr Phe Gly Ile Gly Asn His Ile Ala Val Arg Ala Glu Gly Leu Thr 195 200 205 Arg His Ser Asp Asn Tyr Arg Val Pro Gly Ile Asn Leu Gly Glu Arg 210 215 220 Leu Asn Tyr Val Pro Asp Thr Tyr Asn Lys Ser Lys Val Gly Thr Leu 225 230 235 240 Gly Leu Ser Phe Val Gly Glu Arg Gly Tyr Ile Gly Ala Ser Tyr Ser 245 250 255 Lys Arg Arg Asp Asn Tyr Gly Leu Pro Gly His Asn His Lys Phe Asp 260 265 270 Phe Cys Thr Gly His Ile Tyr Gly Asn Lys Arg Asp Lys Tyr Ala Tyr 275 280 285

Thr Tyr Leu Tyr Pro His Leu Ile Gly Glu Glu Asn Ile Gly Ser Asn 290 295 300 Pro His Phe His Cys Gly Thr Asn His Ala Glu Asp Gly Thr His Ser 305 310 315 320 His Asp Asn Pro Phe Gly His Ala His Asp His Thr His Lys Gly Pro 325 330 335 Trp Val Asp Leu Glu Ser Lys Arg Ile Asp Val Lys Ala Glu Leu Arg 340 345 350 Gln Pro Phe Lys Gly Val Asp Lys Ile Lys Ala Ser Tyr Ala Asp Ala 355 360 365 Asp Tyr Tyr His Asp Glu Lys Asp Ala Gly Val Leu Ala Thr Arg Tyr 370 375 380 His Lys Gln Leu Lys Lys Asp Gln Asp Tyr Gly Lys Pro Val Asn Ile 385 390 395 400 Phe Lys Asn Arg Gly Lys Asn Thr Arg Leu Glu Val Tyr His Ala Pro 405 410 415 Leu Gly Gly Leu Thr Gly Val Trp Gly Val Gln Tyr Gln Thr Gln Lys 420 425 430 Ser Ser Met Asn Ala Pro Lys Asp Arg Glu Val Lys Phe Pro Leu Val 435 440 445 Glu Asn Thr Asn Lys Gln Phe Ser Leu Phe Gly Val Glu Gln Tyr Met 450 455 460 Trp Asp Ser Val Ala Val Glu Leu Ala Gly Arg Met Glu Lys Gln Lys 465 470 475 480 Ile Glu Ile Glu Tyr Asp Arg Asn Glu Ile Lys Arg Leu Gln Glu His 485 490 495 Tyr Arg Ile Ser Gly Gly Lys Gln Val Glu Pro Asp Leu Ser Pro Tyr 500 505 510 Asp Glu Thr Ala Tyr Ala Tyr Ser Gly Thr Leu Asn Trp Phe Phe His 515 520 525 Pro Asp Tyr Gln Leu Ser Phe Thr Ala Ser His Asn Glu Arg Leu Pro 530 535 540 Thr Pro Met Glu Leu Tyr Tyr His Gly Gln His Ile Ala Thr Asn Ser 545 550 555 560 Phe Glu Tyr Gly Asn Lys Asp Leu Lys Lys Glu Gln Ser Asn Asn Val 565 570 575 Glu Leu Gly Leu Gly Tyr Gln Ala Glu Arg Ile Gly Tyr Lys Val Ser 580 585 590 Val Tyr Tyr Asn His Phe Lys Asn Tyr Ile Tyr Asn Glu Asn Leu Phe 595 600 605 Arg Glu Asn Gln Leu Phe Met Arg Arg Tyr Asn Gln Ala Lys Ala Arg 610 615 620 Phe Tyr Gly Val Glu Ala Glu Ala Ser Tyr Arg Phe Asn Asp Gln Tyr 625 630 635 640 Gln Ala Thr Ile Phe Gly Asp Met Val Arg Gly Trp Leu Thr Asn Leu 645 650 655 Pro Pro Leu Lys Ile Asn Ser Asp Tyr Ser Val Phe Lys Asp Tyr Leu 660 665 670 Pro Glu Asn Val Glu Lys Gly Lys Asp Tyr Leu Leu Tyr Arg Ala Asp 675 680 685 Gln Asn Thr Pro Arg Thr Pro Pro Met Arg Leu Gly Phe Arg Phe Asn 690 695 700 Ala Glu Phe Thr Pro Asn Trp Ser Gly Asp Leu Glu Leu Ile Arg Thr 705 710 715 720 Phe Thr Gln Arg Arg Thr Ser Gln Leu Glu Tyr Ile Thr Glu Gly Asn 725 730 735 Thr Met Leu Asn Val Gly Leu Ser Tyr Ser Asn Lys Trp Lys Glu Leu 740 745 750 Asp Tyr Lys Ile Ser Leu Asn Gly Thr Asn Leu Leu Asn Gln Pro Val 755 760 765 Tyr Ile His Thr Ser Tyr His Gln Phe Val Pro Gln Met Gly Arg Asn 770 775 780 Phe Met Leu Gly Met Glu Met Lys Phe 785 790 42818PRTMoraxella catarrhalis 42Met Lys Val Thr Met Ile Lys Lys Pro Leu Ala Cys Ala Ile Leu Ala 1 5 10 15 Thr Phe Ser Met Pro Met Leu Ala Glu Ala Asn Leu Lys Asp Lys Pro 20 25 30 Thr Val Ile Leu Asp Gly Val Ser Ile Thr Ser Leu Ala Asp Gln Asn 35 40 45 Thr Glu Phe Gly Val Asn His Ser Lys Thr Val Ser Gly Ile Thr Val 50 55 60 Ser Lys Glu Gln Leu Gln Gln Arg Ala Thr Thr Leu Gly Asp Ala Leu 65 70 75 80 Ala Gly Glu Leu Gly Val His Ser Asn His Phe Gly Gly Gly Ala Ser 85 90 95 Ala Pro Ile Ile Arg Gly Gln Glu Gly Lys Arg Leu Lys Ile Leu Gln 100 105 110 Asn Gly Ser Glu Val Val Asp Met Ser Gly Leu Ser Pro Asp His Ala 115 120 125 Ile Ala Val Asp Thr Thr Leu Ala Lys Gln Val Glu Ile Val Arg Gly 130 135 140 Ser Gly Ala Leu Leu Tyr Ala Ser Gly Asn Ser Ala Gly Val Val Asn 145 150 155 160 Val Val Asp Asp Lys Ile Pro Ser Lys Leu Pro Ser Lys Leu Gln Gly 165 170 175 Asp Val Thr Val Arg Leu Ser Ser Ala Asn Arg Glu Lys Leu Ile Thr 180 185 190 Ala Ser Ala Glu Ala Pro Leu Gly Glu His Val Ala Val Arg Val Ala 195 200 205 Gly Leu Ser Lys Gln Ala Ala Asp Tyr Lys Thr Pro Arg Phe Asp Arg 210 215 220 His Val Phe Asn Lys Lys His Glu Asp Asp Asn Thr Gln Pro Glu Phe 225 230 235 240 Ile Tyr Lys Asp Thr Leu Lys His Leu Pro Asp Ser His Ala Lys Ser 245 250 255 Asn Ala Gly Thr Leu Gly Val Ser Trp Val Gly Asn Gln Gly Phe Leu 260 265 270 Gly Ala Ser Val Ser Leu Arg Arg Asp Lys Tyr Gly Leu Pro Asn His 275 280 285 Ser His Glu Tyr Glu Glu Cys Ser Val His Gly Ile Ser Gln Ser Ala 290 295 300 Leu Gln Tyr Lys Pro Tyr Leu Arg Leu Tyr Pro Phe Leu Met Glu Asn 305 310 315 320 Asp Asp Leu Glu Phe Asp Asn Ala Gly Leu Glu Cys His Thr His Asp 325 330 335 Asp His Asp His Glu His Asp His Ala His Asp His Glu His Asp His 340 345 350 Glu His Asp His Gly Lys Pro Trp Ile Asp Leu Lys Met Lys Arg Tyr 355 360 365 Asp Val Gln Gly Gln Ile Asn Ala Pro Phe Ala Gly Ile Asp Lys Ile 370 375 380 Arg Ala Ser Met Gly Lys Val Asp Tyr His His Asp Glu Ile Asp Gly 385 390 395 400 Gly Glu Lys Thr Ser Phe Phe Asp Asn Gln Ala Asn Val Trp Arg Leu 405 410 415 Glu Ala Ser His Thr Pro Ile His Thr Pro Met Gly Lys Phe Ser Gly 420 425 430 Val Phe Gly Val Gly Tyr Leu Thr Ser Lys Asn Ser Gly Leu Val Pro 435 440 445 Pro Arg Tyr Glu Asp Gly Asn Lys Gln Asp Thr Gln Asn Ile Leu His 450 455 460 Asn Asn Lys Thr Lys Thr Gly Ser Val Phe Trp Phe Glu Glu Tyr Lys 465 470 475 480 Pro Asn Asp Lys Leu Thr Val Asp Ala Ala Ala Arg Ile Glu Lys Gln 485 490 495 Thr Ile Thr Met Asp Tyr Asp Lys Asp Ala Ile Tyr Gln Ser Leu Asn 500 505 510 Leu Gly Leu Ala Thr Ala His Glu Pro Asp Ile Arg Phe Lys Arg Leu 515 520 525 Leu Asp Ser Gly Thr Leu Asn Pro Lys Lys Gln Thr Ala Arg Ser Tyr 530 535 540 Ala Val Gly Thr His Leu Gln Leu Thr Pro Lys His Lys Leu Ser Leu 545 550 555 560 Asn Leu Ser His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala 565 570 575 His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly Asn Arg Phe 580 585 590 Leu Asn Lys Glu Lys Ser Asn Asn Ile Asp Leu Gly Leu Thr Phe Gln 595 600 605 Gly Asp Lys Trp Asp Tyr Arg Leu Gly Gly Tyr His Tyr Asp Phe Asp 610 615 620 Asn Tyr Val Phe Leu Gln Thr Leu Ser Gln Tyr Lys Gln Gly Leu Arg 625 630 635 640 Gly Met Arg His Asp Lys Asp Leu Lys Thr Ala Arg Tyr Glu Gln Ala 645 650 655 Ala Ala Lys Phe Tyr Gly Phe Asp Val Asn Ile Gly Tyr Gln Ile Asn 660 665 670 Asp Val Tyr His Val Ala Leu Phe Gly Asp Tyr Ile Arg Gly Lys Leu 675 680 685 Thr Asn Leu Pro Asp Lys Lys Gly Arg Thr Asp Ala Tyr Gly Asn Arg 690 695 700 Pro Leu Ile Lys Gln Pro Asp Ser His Thr Pro Arg Leu Pro Pro Lys 705 710 715 720 Arg Leu Gly Met Lys Leu Thr Ala Asn Val Asn Ala Asn Trp Ser Gly 725 730 735 Phe Leu Glu Tyr Arg His Thr Phe Lys Gln Asp Lys Leu Ala Asn Phe 740 745 750 Glu Arg Pro Thr Pro Ala His Asn Leu Val Asn Leu Gly Leu Asn Tyr 755 760 765 Gln His Lys Pro Ser His Gln Ala Gly Ser Val Gln Val Phe Phe Asn 770 775 780 Ala Asn Asn Leu Leu Asn Asp Lys Val Phe Ala His Glu Thr Phe Phe 785 790 795 800 Pro Asp Met Pro Gln Met Gly Arg Asn Phe Met Leu Gly Ala Asn Phe 805 810 815 Lys Phe 43796PRTPasteurella dagmatis ATCC 43325 43Met Lys Lys Ile Ser Tyr Leu Ser Leu Cys Val Ile Ser Gly Leu Tyr 1 5 10 15 Ser Gln Ile Ala Val Ala Gln Asn Asn Leu Thr Asn Thr Gly Asn His 20 25 30 Ile Glu Leu Asp Ala Ile His Val Asp Ala Leu Asn Asp Ser Arg Gln 35 40 45 Gly Ala Pro Leu Thr Gly Arg Leu Ile Thr Ser Glu Lys Thr Ile Ser 50 55 60 Glu Tyr Ser Leu Lys Gln Arg Gly Ser Asn Ile Gly Asp Ala Leu Ser 65 70 75 80 Asn Glu Leu Gly Ile His Ala Ser Gln Phe Gly Gly Gly Ala Ser Ala 85 90 95 Pro Val Ile Arg Gly Gln Glu Gly Lys Arg Ile Lys Leu Leu Asn Ser 100 105 110 Gly Thr Glu Thr Leu Asp Met Ser Ser Met Ser Pro Asp His Ala Val 115 120 125 Thr Val Asp Ser Leu Leu Ala Lys Lys Ile Glu Ile Leu Arg Gly Val 130 135 140 Asn Thr Leu Leu Tyr Ser Ser Gly Asn Ala Ala Gly Ala Val Asn Val 145 150 155 160 Val Asp Asn Lys Ile Pro Thr Thr Glu Ile Val Gly Val Glu Gly Glu 165 170 175 Val Gly Leu Arg Thr Gly Ser Val Asp Asn Glu Arg Leu Val Asn Ala 180 185 190 Ala Phe Asp Val Gly Leu Ser Lys His Phe Ala Leu His Leu Glu Gly 195 200 205 Leu Tyr Lys Lys Ala Gly Asp Tyr Arg Thr Pro Ser Tyr Gln Tyr Gln 210 215 220 Gly Ser Thr Gln His Lys Leu Ala Asn Ser Phe Val Asp Asn Arg Ser 225 230 235 240 Gly Ser Ile Gly Leu Ser Trp Val Gly Asp Lys Gly Tyr Leu Gly Ala 245 250 255 Ser Tyr Ser Gln Arg Lys Asp Lys Tyr Gly Leu Pro Ala His Ser His 260 265 270 Leu Tyr Asp Glu Tyr Tyr Met His Val Leu Leu Ser Asp Ala His Trp 275 280 285 Arg Lys Pro Tyr Leu Lys His Tyr Pro Phe Leu Met Glu Glu Thr Asp 290 295 300 Ile Asp Tyr Asn Asn Pro Gly Ile Asp Cys Ile Lys Lys Ser Trp His 305 310 315 320 Ser His Gly His Leu Cys Asn His Gly His Ala His His Ala Asn Glu 325 330 335 Pro Asn Ser His Asp His Gln His Ala Asp Pro His Ile Val Leu Asn 340 345 350 Ser Gln Arg Trp Asp Ile Arg Gly Glu Trp Lys Asn Pro Val Lys Gly 355 360 365 Leu Asp Lys Val Arg Phe Ser Val Ala Gln Val Asp Tyr Arg His Asp 370 375 380 Glu Lys Ser Gly Ala Ile Ser Asp Asn Ser Phe Lys Asn Lys Gly Tyr 385 390 395 400 Ser Ala Arg Leu Glu Phe Ile His Gln Pro Ile Ala Asn Val Ser Gly 405 410 415 Leu Ile Gly Val Ser His Thr Tyr Gln Asp Ser Tyr Ala Leu Asp Asn 420 425 430 His Thr Leu Glu Tyr Arg Lys Gln Asn Leu Leu Ser Asp His Thr Thr 435 440 445 Asp Gln Gln Ser Leu Phe Leu Met Glu Arg Val Glu Phe Gly Lys Trp 450 455 460 Gln Phe Asp Ile Gly Gly Arg Met Glu Arg Gln Arg Ile Ala Met Lys 465 470 475 480 Tyr His Phe Asn Val Pro Ala Arg Glu Gln Val Pro Gln Glu Leu Thr 485 490 495 Gln Pro His Lys Ser Lys Gly Tyr Ser Tyr Ala Phe Ser Ala Asn Tyr 500 505 510 Gln Val Asn Asp Arg His Gln Leu Asn Ile Ile Phe Ser His Gln Glu 515 520 525 Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His Gly Lys His Leu Ala 530 535 540 Thr Asn Ser Phe Glu Ala Gly Asn Lys Asn Leu Lys Lys Glu Arg Ser 545 550 555 560 Asn Asn Ile Glu Leu Gly Trp Asp Tyr Ser Gly Glu Lys Leu Gly Ile 565 570 575 Lys Leu Ser Gly Tyr Tyr Gln Gln Phe Ser Asn Tyr Ile Tyr Ala Ala 580 585 590 Ile Leu Asn Asp Lys Ser Thr Cys Thr Trp Arg Ala Asn Gly Arg Cys 595 600 605 Ser Arg Ser Leu Ser Ser Asp Tyr Pro Leu His Leu Tyr Arg Tyr Asn 610 615 620 Gln Ala Glu Ala Asn Ile Tyr Gly Leu Glu Ala Glu Ala Ser Tyr Gln 625 630 635 640 Ile Ser Ser Thr Tyr Asp Val Ser Ile Phe Gly Asp Tyr Val Arg Gly 645 650 655 Lys Leu Lys Asn Leu Pro Val Leu Pro Ile Gly Tyr Glu Gln Ala Tyr 660 665 670 Asp Gln Asn Tyr Asn Pro Ile Gly Leu Lys Pro Thr Gly Trp Glu Lys 675 680 685 Gln Pro Asp Gly Asn Ala Pro Arg Met Pro Ala Met Arg Leu Gly Ile 690 695 700 Lys Leu Asn Gly His Phe Asp Asn Gly Val Ser Phe Asn Thr Gln Leu 705 710 715 720 Tyr Arg Val Phe Lys Gln Asn Lys Val Ala Arg Leu Glu Lys Pro Thr 725 730 735 Pro Gly His Thr Met Trp Asn Ala Gly Val Ser Tyr Asp Gly Arg Ile 740 745 750 Gly Asn Asn Glu Tyr Thr Leu Phe Leu Asn Ala Asn Asn Leu Leu Asn 755 760 765 Val Lys Val Tyr Asn His Ala Ser Phe Leu Ser Tyr Ile Pro Gln Asn 770 775 780 Gly Arg Gly Ile Asn Val Gly Met Asn Phe Lys Phe 785 790 795 44809PRTPasteurella multocida subsp. multocida str. Pm70 44Met Pro Leu Leu Thr Leu Lys Ile Asn Met Phe Phe Met Arg Lys Ile 1 5 10 15 Ser Tyr Leu Ser Leu Cys Val Ile Ser Ala Leu Tyr Ser Gln Leu Ala 20 25 30 Val Ala Gln Ser Pro Leu Lys Asn Thr Ser Glu His Ile Glu Leu Glu 35 40 45 Pro Ile Phe Val Asn Thr Leu Ile Glu Ser Arg Glu Gly Ala Pro Leu 50 55 60 Gly Gly Arg Leu Met Ala Ser Glu Lys Ile Ile Pro Ala Tyr Ser Leu 65 70 75 80 Lys Gln Arg Gly Ser Asn Leu Gly Asp Ala Leu Ser Ser Glu Leu Gly 85 90 95 Ile His Ala Ser Gln Phe Gly Gly Gly Ala Ser Ala Pro Val Ile Arg 100 105 110 Gly Gln Glu Gly Lys Arg Ile Lys Val Leu Ser Ser Gly Asn Glu Thr 115 120 125 Leu Asp Met Ser Ala Met Ser Pro Asp His Ala Val Ala Val Asp Ser 130 135 140 Leu Leu Ala Lys Lys Val Glu Ile Leu Arg Gly Ala Asn Thr Leu Leu 145 150 155 160 Tyr Ser Ser Gly Asn Ala Ala Gly Val Val Asn Val Val Asp Asn Lys 165

170 175 Ile Pro Thr Ala Glu Val Val Gly Val Glu Gly Glu Val Gly Leu Arg 180 185 190 Thr Gly Ser Ala Asp Asn Glu Arg Leu Val Asn Val Ala Leu Asp Val 195 200 205 Gly Leu Ser Lys His Phe Ala Leu His Leu Glu Gly Leu His Lys Lys 210 215 220 Ala Gly Asp Tyr Arg Thr Pro Ser Tyr Gln Tyr Gln Gly Ser Thr His 225 230 235 240 His Lys Leu Ala Asn Ser Phe Val Asp Asn Arg Ser Gly Ser Val Gly 245 250 255 Leu Ser Trp Val Gly Asp Lys Gly Tyr Leu Gly Val Ala Tyr Ser Gln 260 265 270 Arg Lys Asp Lys Tyr Gly Leu Pro Ala His Ser His Leu Tyr Asp Glu 275 280 285 Tyr Tyr Met His Val Leu Leu Ser Asp Ala His Trp Arg Lys Pro Tyr 290 295 300 Leu Lys His Tyr Pro Phe Leu Met Glu Glu Thr Asp Ile Asp Tyr Asn 305 310 315 320 Asn Pro Gly Ile Asp Cys Ile Lys Lys Glu Trp His Ser His Gly His 325 330 335 Leu Cys Asn His Gly His Ala His His Gly Asn Gly Gln His Ser His 340 345 350 Asp His His Ala His Ala Asp Pro His Ile Ala Leu Asn Thr Gln Arg 355 360 365 Trp Asp Leu Arg Gly Glu Trp Lys Asn Pro Val Lys Gly Leu Asp Lys 370 375 380 Val Arg Phe Ser Ile Ala Lys Val Gly Tyr Arg His Asp Glu Lys Ser 385 390 395 400 Gly Ala Ile Ser Asp Asn Ser Phe Lys Asn Lys Gly Tyr Ser Ala Arg 405 410 415 Val Glu Phe Leu His Gln Pro Ile Ala Gly Val Ser Gly Leu Ile Gly 420 425 430 Leu Ser His Val Tyr Gln Asp Ser Tyr Ala Leu Asp Asn His Thr Leu 435 440 445 Glu Tyr Arg Lys Gln Asn Leu Leu Ser Asp His Thr Thr Ala Gln Gln 450 455 460 Ser Leu Phe Leu Met Glu His Val Glu Leu Gly Lys Trp Gln Phe Asp 465 470 475 480 Ile Gly Gly Arg Val Glu Lys Gln Arg Ile Ala Met Lys Tyr His Phe 485 490 495 Asn Val Pro Lys Asp Glu Gln Pro Pro Glu Glu Leu Thr Arg Pro His 500 505 510 Lys Ser Lys Ala Tyr Ser Tyr Ala Leu Ser Ala Asn Tyr Gln Leu Asn 515 520 525 Glu Gln His Gln Phe Asn Met Ile Val Ser His Gln Glu Arg Leu Pro 530 535 540 Asn Ala Gln Glu Leu Tyr Ala His Gly Lys His Leu Ala Thr Asn Ser 545 550 555 560 Phe Glu Ala Gly Asn Lys Asn Leu Thr Lys Glu Arg Ser Asn Asn Val 565 570 575 Glu Leu Gly Trp Gly Tyr Thr Gly Glu Lys Leu Gly Ile Lys Leu Ser 580 585 590 Gly Tyr Tyr Gln Gln Phe Ser Asn Tyr Ile Tyr Ala Ala Ile Leu Asn 595 600 605 Asn Lys Thr Ser Cys Pro Trp Arg Pro Asn Ser Arg Cys Leu Arg Ser 610 615 620 Leu Ser Asp Asp Tyr Pro Leu Arg Leu Tyr Arg Tyr Asn Gln Ala Lys 625 630 635 640 Ala Lys Ile Tyr Gly Leu Glu Ala Glu Val Ser Tyr Gln Ile Ser Ser 645 650 655 Thr His Ser Val Ser Ile Phe Gly Asp Tyr Val Arg Gly Lys Leu Lys 660 665 670 Asp Leu Pro Ser Leu Pro Ile Gly Tyr Lys Tyr Ile Tyr Asn Glu Asn 675 680 685 Tyr Asp Met Val Gly Val Gln Pro Thr Gly Trp Glu Lys Gln Pro Asp 690 695 700 Gly Asn Ala Pro Arg Met Ser Pro Met Arg Leu Gly Ile Lys Trp Asn 705 710 715 720 Ala Tyr Phe Asp Asn Gly Ile Ser Phe Asn Thr Gln Leu Tyr Arg Val 725 730 735 Phe Ala Gln Asn Lys Val Ala Arg Leu Glu Thr Pro Thr Lys Gly His 740 745 750 Thr Met Leu Asn Leu Gly Met Ser Tyr Asp Gly Lys Met Gly Asn Asn 755 760 765 Glu Tyr Thr Leu Phe Ala Asn Val Asn Asn Val Leu Asn Ser Arg Val 770 775 780 Tyr Asn His Thr Ser Phe Leu Ser Tyr Ile Pro Gln Ser Gly Leu Gly 785 790 795 800 Leu Asn Val Gly Met Asn Phe Lys Phe 805 45805PRTPasteurella multocida subsp. multocida str. Pm70 45Met Ile Ser Arg Gly Cys Lys Val Asn Lys Phe Phe Ala Val Leu Met 1 5 10 15 Met Cys Cys Ile Pro Gln Val Val Trp Ala Asn Thr Glu Lys Lys Gln 20 25 30 Ile Val Phe Leu Asp Glu Ile Ser Val Glu Ser Lys Gly Ala Ala Phe 35 40 45 Arg Ser Asp Pro Leu Ser Gly Leu Pro Lys Gln Asn Asp Ile Leu Val 50 55 60 Ser Lys Gln Lys Leu Lys Thr Gly Ser Ser Thr Leu Gly Asn Ala Leu 65 70 75 80 Ala Gly Glu Leu Ser Val His Ser Asn Gln Phe Gly Gly Gly Ser Ser 85 90 95 Ala Pro Val Val Arg Gly Gln Glu Gly Val Arg Leu Lys Ile Leu Gln 100 105 110 Asn Gly Ser Asp Val Ile Asp Met Ser Gln Leu Ser Pro Asp His Ala 115 120 125 Ile Gly Val Asp Thr Leu Leu Ala Glu Gln Val Glu Ile Val Arg Gly 130 135 140 Ala Ser Thr Leu Leu Tyr Ala Asn Ala Ser Pro Ala Gly Val Ile Asn 145 150 155 160 Val Val Asp Lys Arg Ile Pro Thr Gln Leu Pro Lys Lys Gly Tyr Glu 165 170 175 Val Asp Phe Asn Thr Arg Tyr Asn Thr Asn Ser His Glu Lys Leu Val 180 185 190 Thr Ala Ala Leu Thr Phe Gly Leu Gly Lys His Ile Ala Leu Arg Val 195 200 205 Glu Glu Leu Leu Arg Gly Ser Asn Asn Tyr His Val Pro Ala Phe Lys 210 215 220 Leu Asp Lys Thr Leu Asn Tyr Val Pro Asp Thr Gln Asn Lys Thr Lys 225 230 235 240 Ser Gly Asn Tyr Gly Val Ala Phe Ile Gly Glu Arg Gly Tyr Val Gly 245 250 255 Phe Ala Tyr Asn Leu Arg Arg Glu Lys Tyr Gly Leu Pro Gly His Asn 260 265 270 His Lys Leu Asp Ser Cys Ala Ala His Ile Trp Gly Gly Asn Val Arg 275 280 285 Asn Asp Tyr Tyr Leu Gly Leu Tyr Pro His Leu Met His Asp Thr Asp 290 295 300 Leu Val Asn Thr His Phe His Cys Gly Ser Asn His Asp Met Asp Gly 305 310 315 320 Lys His Ser His Asn His Pro Tyr Gly His Asp His Asp His Ser Ile 325 330 335 Ala Gly Pro Leu Ile Asp Ser Tyr Ala Lys Arg Tyr Asp Ile Arg Ala 340 345 350 Glu Val Lys Gln Pro Met Lys Ala Ile Glu Lys Ile Lys Leu Ser Tyr 355 360 365 Ser Glu Thr Arg Tyr Lys His Asp Glu Lys Asp Gly Asn Ile Ala Val 370 375 380 Asn Leu Phe Lys Asn Asn Gly Tyr Asn Leu Arg Val Glu Ile Phe His 385 390 395 400 Thr Pro Ile Ala Gly Leu Ser Gly Val Ile Gly Ala Gln Tyr Gln Thr 405 410 415 Gln Thr Ser Ser Ala Asn Ile Pro Arg Ile Ala Pro Cys Ser Asn Asn 420 425 430 Ala Ser Asp Pro Cys His Lys Lys Lys Gln Arg Asp Pro Ser Lys Ile 435 440 445 Thr Lys Gly Asp Arg Lys Ser Trp Ala Leu Ile Glu Asn Thr Gln Ser 450 455 460 Gln Met Ser Phe Phe Ala Ile Glu Gln Leu Arg Trp Gln Asp Phe Leu 465 470 475 480 Phe Glu Ile Gly Val Arg Thr Glu Lys Gln Arg Ile Asp Ile Glu Tyr 485 490 495 Asp Arg Ala Trp Leu Phe Lys Val Lys Arg Lys Leu Glu Gly Cys Asp 500 505 510 Pro Asn Ser Phe Phe Tyr Ser Pro Ser Gly Cys Arg Gln Gly Ser Tyr 515 520 525 Pro Ala Pro Asp Phe Ala Ser Tyr His Asp Arg Ala Thr Ser Tyr Ser 530 535 540 Gly Ala Ile Ser Trp Asn Met Thr Pro Asp Tyr Thr Leu Ser Leu Thr 545 550 555 560 Tyr Ser His Asn Glu Arg His Pro Thr Pro Met Glu Leu Tyr Tyr His 565 570 575 Gly Lys His Leu Ala Thr Val Ser Phe Glu His Gly Asn Arg Asn Leu 580 585 590 Lys Lys Glu Val Ser Asp Asn Trp Glu Val Gly Leu Ala Tyr Leu Gly 595 600 605 Asp Lys Leu Ser Tyr Lys Val Asn Val Tyr Tyr Asn Asp Phe Lys Asn 610 615 620 Arg Ile Phe Asn Gln Thr Leu Asn Lys Ser Gly Asn Leu Ser Leu Asn 625 630 635 640 Arg Tyr Asn Gln Ser Lys Ala Lys Tyr Tyr Gly Val Glu Gly Arg Ile 645 650 655 Asp Tyr Ala Leu Thr Pro Glu Leu His Met Gly Leu Phe Gly Asp Tyr 660 665 670 Val Arg Gly Lys Leu Tyr Asp Leu Pro Pro Thr Tyr Arg Val Asp His 675 680 685 Val Ala Asn Ser Leu Glu Pro Val Thr Gln Pro Asp Gln Asp Ala Pro 690 695 700 Arg Val Pro Pro Met Arg Leu Gly Phe Arg Val Asn Met Glu Met Thr 705 710 715 720 Glu Ser Leu Thr Ser Ser Leu Glu Tyr Thr Tyr Val Tyr Gln Gln Lys 725 730 735 Lys Val Ala Pro Leu Glu Asn Gln Thr Ala Ala Tyr Ser Leu Leu Asn 740 745 750 Ile Gly Val Asp Tyr Ser Arg Gln Ile Ala Gly Val Asn Tyr Gln Leu 755 760 765 Phe Val Gln Ala Asn Asn Val Leu Asn Arg Lys Val Tyr Ser His Thr 770 775 780 Ser Phe Leu Pro Phe Val Pro Gln Met Gly Arg Asn Val Thr Leu Gly 785 790 795 800 Leu Asn Ile His Phe 805 46925PRTPasteurella multocida subsp. multocida str. Pm70 46Met Val Asn Ile Leu Tyr Phe Leu Tyr Lys Lys Trp Ile Phe Leu Leu 1 5 10 15 Gln Asn Asn Ile Phe Met Leu Asn Leu Ser Thr Leu Phe Phe Tyr Val 20 25 30 Lys Lys Asp Val Glu Thr Val Glu Thr Lys Val Val Thr Asn Lys Leu 35 40 45 Ile Lys Gln Ile Thr Tyr Leu Leu Pro Trp Ser Ala Val Ala Phe Thr 50 55 60 His Val Ile Tyr Ala Gln Thr Glu Met Leu Asp Glu Ile Val Val Ser 65 70 75 80 Gly Ala Gln Pro His Leu Ala Gly Ser Ala Ile Glu His Tyr Gln Ala 85 90 95 Ile Ser Asn Gln Val Ile Lys Lys Glu Arg Leu Gln Lys Gln Ser Ala 100 105 110 Thr Leu Gly Asn Ala Leu Ala Gly Glu Leu Gly Val His Ser Asn Pro 115 120 125 Phe Gly Gly Gly Ala Ser Ala Pro Ile Ile Arg Gly Gln Glu Gly Val 130 135 140 Arg Ile Lys Ile Leu Gln Asn Gly Leu Asp Val Val Asp Met Ser Ala 145 150 155 160 Ile Ser Pro Asp His Ala Val Ala Ala Asp Ser Leu Leu Ala Glu Gln 165 170 175 Val Glu Leu Val Arg Gly Ala Ser Thr Leu Leu Tyr Ser Ser Ala Ser 180 185 190 Ala Ala Gly Val Val Asn Val Val Asp Lys Arg Ile Pro Thr Ala Val 195 200 205 Pro Glu Lys Gly Tyr Glu Gly Glu Ile Phe Thr Arg Phe Asp Thr Ala 210 215 220 Ser Gln Glu Ser Thr Gly Thr Ala Gly Ile Thr Phe Arg Leu His Pro 225 230 235 240 His Leu Ala Leu Arg Leu Glu Gly Leu Lys Arg Tyr Ser Thr His Tyr 245 250 255 Arg Val Pro Ala Phe Lys Ser Gly Gly Glu Thr Ile Arg Tyr Leu Pro 260 265 270 Asp Ser His Asn Arg Ser Gln Val Gly Thr Ile Gly Val Ser Trp Ile 275 280 285 Lys Asp Gln Ser Tyr Leu Gly Val Ser Tyr Ser Glu Arg Arg Asp Arg 290 295 300 Tyr Gly Leu Pro Gly His Asn His Lys Tyr Asp Arg Cys Lys Ser His 305 310 315 320 Val Val Asp Glu Ala Ala Arg Pro Glu Leu Gly Lys Gly Tyr Leu Thr 325 330 335 Pro Tyr Pro His Leu Ala Asp Asp Thr Asp Ile Val Phe Ala His Leu 340 345 350 Asp Gly Cys Ile Gly Gly Ile Asp Asn Asp Pro Ser His Ser His Asp 355 360 365 His Pro Phe Gly His Glu His Asp His Ser His Gly Gly Pro Trp Val 370 375 380 Arg Leu His Ser Arg Arg Phe Asp Leu Arg Gly Gln Trp Asp Ser Pro 385 390 395 400 Thr Ala Trp Leu Asp Lys Val Lys Gly Ser Phe Ser Tyr Ala Asp Tyr 405 410 415 Ile His Tyr Glu Tyr His Ser Gly Gln Ala Gly Thr Asp Lys Phe Asp 420 425 430 Arg Asp Ser Phe Ile Glu Arg Glu Arg Lys Lys Ala Glu Lys Asn Arg 435 440 445 Gly Lys Ala Ala Gly Ile Tyr Lys Asn Ser Gly Tyr Asn Gly Lys Leu 450 455 460 Glu Phe Tyr His Thr Pro Ile Val Gly Leu Ser Gly Val Phe Gly Val 465 470 475 480 Gln Tyr Ser Glu Tyr Lys Thr Ala Ile Leu Ala Pro Leu Gly Ser Gly 485 490 495 Ile Lys His Gln His His Leu Val Pro Asn Thr Gln Lys Gln Ala Ser 500 505 510 Phe Phe Ala Val Glu Asn Tyr Val Val Asp Asp Phe Ile Phe Glu Val 515 520 525 Gly Ala Arg Val Asp Lys Gln Arg Ile Pro Ile Lys Tyr Asp Gln His 530 535 540 Val Leu Asn Ala His Lys Lys Glu Gly Asp His Pro Pro Asp Leu Ser 545 550 555 560 Thr His Lys Glu Lys Ala Val Ser Tyr Leu Gly Ser Val Asp Trp Leu 565 570 575 Phe His Pro Asn Tyr Arg Ile Gly Leu Thr Leu Ser Arg Asn Glu Arg 580 585 590 Leu Pro Thr Pro Met Glu Leu Tyr Tyr His Gly Gln His Leu Ala Thr 595 600 605 Asn Ser Phe Glu His Gly Asn Lys Asn Leu Arg Lys Glu Ser Ser Asn 610 615 620 Asn Ile Glu Leu Gly Phe Ala Tyr His Thr Asp Lys Trp Asp Tyr Lys 625 630 635 640 Leu Ser Leu Tyr Gln Asn Lys Phe Arg Asn Tyr Ile Tyr Asn Glu Asp 645 650 655 Leu Ala Arg Tyr Gly Asn Ala Phe Leu Arg Arg Tyr Thr Gln Ala Arg 660 665 670 Ala Lys Phe His Gly Ile Glu Ala Glu Leu Asn Phe Arg Pro Thr Pro 675 680 685 Asp Tyr Gln Val Thr Leu Phe Gly Asp Tyr Val Arg Gly Arg Leu Phe 690 695 700 Asp Leu Pro Glu Gln Tyr Gly Gln Arg Phe Tyr Gln Gly Tyr Ile Ala 705 710 715 720 Tyr Asp Glu Glu Gly Leu Ile Gln Ala Asn Trp Glu Lys Gln Pro Tyr 725 730 735 Arg Ile Glu Gly Ile Lys Arg Asn Glu Arg Asp Ala Pro Arg Val Pro 740 745 750 Pro Ala Arg Leu Gly Met Arg Leu Ser Gly Asn Val Thr Glu His Leu 755 760 765 Ser Phe Phe Ala Asp Tyr Thr Tyr Val Phe Ser Gln Gln Lys Thr Ala 770 775 780 Ser Ser Leu Ser Ile Lys Pro Pro Arg Ala Leu Glu Ala Ser Asp Phe 785 790 795 800 Ile Asp Asp Asp Thr Gly Glu Asn Leu Leu Leu Lys Gly Ile Asp Arg 805 810 815 Asp Lys Tyr Asn Arg Thr Gly Gln Pro Ile Ala Asp Leu Ser Ala Ser 820 825 830 Asp Lys Ala Asp Phe Glu Ser Ala Leu Glu Ala Ile Arg Leu Arg Glu 835 840 845

Glu Asn Glu Lys Leu Pro Ala Lys Ile Glu Lys Ile Gln Glu Asp Pro 850 855 860 Ser Lys Gly His His Leu Leu Asn Ile Gly Val Asn Tyr Gln Arg Gln 865 870 875 880 Ile Gly His Leu Asp Tyr Ser Val Gly Phe Ser Val Asn Asn Val Leu 885 890 895 Asn Gln Arg Val Tyr Val His Thr Ser Tyr Leu Pro Tyr Val Pro Gln 900 905 910 Met Gly Arg Asn Tyr Val Leu Asn Phe Gly Val Lys Phe 915 920 925 47697PRTProteus mirabilis ATCC 29906 47Met Ala Asp Ser Met Lys Lys Ser Ile Phe Asn Tyr Thr Thr Phe Phe 1 5 10 15 Ile Ser Met Ser Tyr Ser Leu Phe Ala Asn Ala Ser Pro Ala Ile Lys 20 25 30 Ser Thr Glu Asn Arg Asn Asn Glu Val Leu Glu Val Tyr Ser Thr Pro 35 40 45 Leu His Thr Pro Gln Glu Lys Met Ala Ile Pro Val Ser Val Leu Thr 50 55 60 Gln Glu Asp Leu Arg Ala Asn Arg Ser Ala Ser Ile Ala Glu Thr Leu 65 70 75 80 Thr Ser Leu Pro Gly Phe His Ala Ser Phe Phe Gly Gly Gly Ser Val 85 90 95 His Pro Val Ile Arg Gly Met Ser Gly Asn Arg Val Lys Val Leu His 100 105 110 Ser Gly Ser Asp Leu Met Asp Val Ser Ser Ile Gly Ala Asp His Thr 115 120 125 Ile Thr Ser Glu Pro Phe Leu Ser Gln Arg Ile Glu Val Leu Lys Gly 130 135 140 Pro Ser Thr Leu Leu Tyr Gly Gly Gly Thr Ile Gly Gly Ala Ile Asn 145 150 155 160 Val Ile Asp Ser Lys Ile Pro Met Ser Val Pro Glu Lys Gly Tyr Glu 165 170 175 Gly Glu Leu His Tyr Gln Tyr Asp Ser Val Ser Lys Gly Asn Thr Gly 180 185 190 Ser Val Gly Leu Thr Leu Gly Glu Asn Asn Leu Ala Leu Arg Leu Glu 195 200 205 Gly Thr Lys Arg Tyr Gln Ser Asp Tyr Lys Gln Pro Glu Pro Leu His 210 215 220 Gln Gly Glu Thr Ser Arg Leu Ala Gly Ser Tyr Gln Asp Asn Gln Ser 225 230 235 240 Ala Asn Ile Gly Val Ser Trp Leu Phe Asp Asp Gly Tyr Ile Gly Ile 245 250 255 Gly Tyr Gly Glu Gln His Arg Arg Tyr Gly Leu Pro Gly His Thr His 260 265 270 Phe His Asp His Glu Asp Asp His His His His Ala Pro Ile Arg Pro 275 280 285 Pro Leu Ile Gly His His His His Asn His Asn His Glu Tyr Pro Asp 290 295 300 Glu Gln His Gln His Gly Ile Pro Tyr Ile Val Met Asp Ser Lys Arg 305 310 315 320 Trp Asp Leu Arg Gly Glu Lys Asn Asn Pro Phe Thr Gly Ile Glu Ser 325 330 335 Ile Arg Phe Ser Ala Val Arg Thr Asp Tyr His His Asp Glu Lys Glu 340 345 350 Gly Asn Glu Val Ser Thr Ser Phe Lys Asn Lys Gly Asp Glu Leu Arg 355 360 365 Leu Thr Phe Thr His Gln Pro Ile Trp Gly Trp His Gly Val Ile Gly 370 375 380 Gly Gln Phe Asn Gln Arg Asp Phe Ser Ala Gln Gly Glu Glu Ala Tyr 385 390 395 400 Val Pro Ser Thr Lys Thr Lys Asn His Ser Leu Phe Leu Leu Glu Glu 405 410 415 Tyr Gln Leu Gly Asp Phe Arg Tyr Glu Leu Gly Phe Arg Gln Glu Trp 420 425 430 Gln Ser Leu Leu Asn Arg Glu Thr Gln Lys Asn Asn Lys Gln Ser Ala 435 440 445 Ser Ser Ile Ser Ala Gly Leu Ala Trp Asn Phe Thr Gln Asp Tyr Phe 450 455 460 Leu Asn Leu Ser Leu Ser His Thr Lys Arg Leu Pro Val Ala Glu Glu 465 470 475 480 Leu Tyr Ala Asn Gly Val His Ala Ala Ser Arg Thr Ile Glu Lys Gly 485 490 495 Asp Pro Asn Leu Thr Ala Glu Ala Ala Asn Asn Ile Asp Ile Gly Ile 500 505 510 Thr Lys Val Thr Gly Asp Ile Gln Phe Asn Met Ser Ala Tyr Tyr Asn 515 520 525 Arg Ile Asn Asn Tyr Ile Tyr Gly Gln Phe Thr Gly Thr Glu Leu Asn 530 535 540 Asn Gly Tyr Arg Ser Leu Gln Tyr Val Gln Asn Asp Ala Gln Phe Lys 545 550 555 560 Gly Ile Glu Gly Asn Ile Asp Tyr Tyr Tyr Asn Gln Asp Ser Leu Ile 565 570 575 Gly Ile Ser Gly Asp Tyr Val Arg Ala Asn Leu Leu His Asn Lys Gly 580 585 590 Asn Ile Pro Arg Ile Pro Ala Tyr Arg Leu Ser Thr Tyr Ile Lys His 595 600 605 Ser Phe Thr Glu Asn Val Ile Gly Gln Ile Arg Ile Asp Tyr Phe Gly 610 615 620 Lys Gln Asn Lys Thr Ala Asp Tyr Glu Thr Ser Thr Glu Ala Tyr Asn 625 630 635 640 Thr Val Ser Leu Gly Ser Glu Tyr Ile Gly Tyr Leu Asp Asn Thr Asp 645 650 655 Tyr Thr Leu Tyr Ala Lys Ile Asn Asn Ile Phe Asp Val Lys Gly Lys 660 665 670 Asp Ser Thr Ser Tyr Ile Lys Asp Glu Met Tyr Leu Pro Gly Arg Asn 675 680 685 Ile Ile Leu Gly Ala Thr Leu Thr Phe 690 695 48672PRTPseudomonas stutzeri A1501 48Met Lys Leu Lys Arg His Pro Leu Ala Trp Ala Ile Ser Leu Ala Leu 1 5 10 15 Val Pro Ala Ala Trp Ala Ala Asp Pro Val Glu Leu Gln Ser Val Val 20 25 30 Val Ser Ala Ser Gly Leu Ala Lys Gln Ser His Glu Met Thr Thr Pro 35 40 45 Val Ala Val Met Glu Asn Asp Glu Leu Val Leu Arg Arg Glu Ala Thr 50 55 60 Leu Gly Glu Thr Leu Glu Ser Leu Pro Gly Val Arg Ser Ser Ser Phe 65 70 75 80 Gly Ala Gly Ala Ala Arg Pro Val Ile Arg Gly Leu Asp Gly Ala Arg 85 90 95 Val Lys Val Leu Ser Asp Gly Val Glu Leu Leu Asp Ala Ser Thr Ile 100 105 110 Ser Pro Asp His Ala Val Thr Ser Glu Pro Leu Leu Ala Glu Arg Ile 115 120 125 Glu Val Leu Lys Gly Pro Ala Thr Leu Leu Tyr Gly Gly Gly Ala Ile 130 135 140 Gly Gly Val Val Asn Val Ile Asp Lys Lys Ile Pro Thr Arg Val Pro 145 150 155 160 Glu Lys Gly Tyr Glu Gly Glu Leu Glu Leu Arg Ala Asn Ser Val Ala 165 170 175 Asn Glu Gly Ala Gly Val Phe Gly Ile Thr Ala Gly Ser Gly Asn Phe 180 185 190 Ala Val Arg Ala Glu Gly Thr Lys Arg Gln Ala Asp Pro Tyr Glu Ile 195 200 205 Pro Gly Ser Pro Asn Lys Gln Glu Gly Ser Tyr Asn Asp Thr Asp Ser 210 215 220 Phe Asn Leu Gly Ala Ser Phe Ile Gly Glu Arg Gly Tyr Ile Gly Ala 225 230 235 240 Ala Tyr Gly Glu Gln Asn Asn Arg Tyr Gly Leu Leu Ala His Glu His 245 250 255 Ala Asp Cys His Thr His Gly Ser Asp Trp His Cys Gly Gly His Asp 260 265 270 Asp Asp Asp Asp Asp His Asp His Asp His Glu His Gly Ser Val Pro 275 280 285 Tyr Val Asp Met Arg Gln Lys Arg Trp Asp Leu Arg Gly Glu Leu Ser 290 295 300 Asp Pro Leu Pro Gly Phe Glu Leu Ala Arg Leu Arg Val Gly His Ser 305 310 315 320 Asp Tyr Gln His Lys Glu Ile Glu Gly Gly Glu Val Gly Thr Arg Phe 325 330 335 Asn Asn Asp Ala Thr Asp Ala Arg Leu Glu Leu Thr His Gln Pro Leu 340 345 350 Phe Gly Trp Arg Gly Val Leu Gly Ala Gln Thr Leu Arg Arg Asp Phe 355 360 365 Glu Ala Leu Gly Glu Glu Ala Tyr Val Pro Gln Thr Leu Thr Arg Asn 370 375 380 His Gly Leu Phe Leu Leu Glu Glu Tyr Thr Ala Gly Ala Trp Arg Tyr 385 390 395 400 Glu Leu Gly Leu Arg His Glu Trp Gln Asp Ile Asp Ala Asp Gly Arg 405 410 415 Pro Asp Thr Asp His Ser Gly Thr Ser Met Ser Ala Gly Ala Val Trp 420 425 430 Thr Phe Ala Pro Gln Tyr Ser Leu Gly Phe Ser Leu Thr Arg Ser Gln 435 440 445 Arg Leu Pro Ser Ala Glu Glu Leu Tyr Ala Asn Gly Pro His Ala Ala 450 455 460 Thr Arg Thr Val Glu Leu Gly Asn Val Asp Leu Lys Glu Glu Thr Ser 465 470 475 480 His Asn Ala Glu Val Thr Leu Arg Lys Phe Ala Gly Arg Thr Thr Phe 485 490 495 Ser Leu Ser Val Phe Arg Asn Glu Val Asp Asp Phe Ile Tyr Ala Ala 500 505 510 Asp Thr Gly Asn Asp Ile Gly Gly Gly Tyr Arg Glu Ile Glu Tyr Arg 515 520 525 Gln Gln Asp Ala Val Leu Thr Gly Ala Glu Gly Glu Val Arg Phe Gln 530 535 540 Ala Thr Asp Ala Thr Ala Phe Thr Leu Phe Gly Asp His Val Arg Gly 545 550 555 560 Lys Leu Arg Gly Asp Gly Gly Asp Leu Pro Arg Ile Pro Ala Asp Arg 565 570 575 Leu Gly Val Arg Leu Asp Gln Ser Phe Thr Pro Ala Leu Asn Gly Gln 580 585 590 Leu Glu Phe Tyr Arg Val Gln Arg Gln Asp Asp Leu Ala Asp Tyr Glu 595 600 605 Thr Glu Thr Gly Gly Tyr Asn Met Leu Gly Ala Ser Leu Gly Tyr Ser 610 615 620 Gly Ser Leu Ser Gln Thr Asp Tyr Leu Leu Tyr Leu Lys Ala Asn Asn 625 630 635 640 Leu Leu Asp Glu Lys Ala Arg Gln His Thr Ser Phe Ile Lys Asp Glu 645 650 655 Val Leu Leu Pro Gly Arg Asn Leu Thr Val Gly Val Arg Leu Ala Phe 660 665 670 49686PRTSphingomonas sp. SKA58 49Met Cys Ala Val Ser Ile Ile Ala Ile Thr Pro Gly Ile Ala Leu Ala 1 5 10 15 Gln Ala Gly Gly His Gly Gln Gly Asp Ile Val Val Thr Ala Ala Pro 20 25 30 Leu Gly Gln Lys Ala Asp Glu Thr Thr Thr Pro Val Val Thr Leu Thr 35 40 45 Gly Glu Glu Leu Ile His Arg Arg Ala Ala Thr Leu Gly Glu Thr Leu 50 55 60 Ala Gly Gln Pro Gly Ile Asn Phe Glu Asn Phe Gly Gly Gly Ala Ser 65 70 75 80 Arg Pro Ile Ile Arg Gly Gln Ser Ser Pro Arg Val Gln Val Leu Ser 85 90 95 Asp Ser Ala Asn Val Gln Asp Ala Ser Ala Ile Ser Pro Asp His Asn 100 105 110 Val Thr Gly Glu Pro Leu Leu Leu Arg Arg Ile Glu Val Leu Arg Gly 115 120 125 Pro Ala Thr Leu Leu Tyr Gly Ser Gly Ala Ile Gly Gly Ala Val Asn 130 135 140 Leu Leu Asp Glu Lys Val Pro Thr Tyr Val Pro Glu Gly Gly Ile Thr 145 150 155 160 Gly Ala Ala Glu Gly Arg Leu Gly Thr Gly Asp Asp Glu Arg Ser Leu 165 170 175 Val Gly Gly Ala Thr Val Gly Val Gly Pro Leu Ala Leu Arg Val Glu 180 185 190 Gly Val His Arg Arg Ser Asp Asp Tyr Arg Val Pro Arg Ala Phe Gly 195 200 205 Glu Asp Arg Val His Gly Ser Tyr Asn Asp Thr Ser Thr Phe Ser Val 210 215 220 Gly Gly Ser Trp Ile Gly Pro Asp Gly Tyr Leu Gly Val Ala Tyr Thr 225 230 235 240 Arg Gln Arg Asn Glu Tyr Gly Val Pro Gly His Asn His Asp Tyr Glu 245 250 255 Ser Cys His Pro His Gly Ile Gly Leu His Cys Gly Ser His Gly Glu 260 265 270 Glu Glu Glu His Asp His Asp His Asp His Glu His Asp His Glu Glu 275 280 285 Glu Val Pro Phe Val Lys Leu Arg Ser Asn Arg Phe Asp Ile Arg Ser 290 295 300 Asp Tyr Asn Asn Pro Val Pro Ala Leu Glu Lys Val Arg Phe Arg Leu 305 310 315 320 Ser Phe Thr Asp Tyr Ala His Asp Glu Ile Glu Asp Gly Glu Ala Glu 325 330 335 Asn Thr Phe Leu Asn Lys Ala His Asp Leu Arg Val Glu Leu Thr His 340 345 350 Ala Pro Leu Gly Gly Leu Arg Gly Thr Phe Gly Ile Gln Gln Ser Gly 355 360 365 Ser Arg Phe Gln Ala Ile Ser Gly Gly Val Thr His Thr Leu Asn Thr 370 375 380 Asp Ser Ser Asn Thr Ala Ile Phe Leu Leu Glu Thr Tyr Ser Leu Gly 385 390 395 400 Asn Val Arg Leu Glu Ala Ala Ala Arg Gln Glu Trp Gln Thr Val Lys 405 410 415 Ser Leu Ile Thr Arg Tyr Pro Ser Ile Lys His Lys Pro Phe Ser Ala 420 425 430 Ser Ala Ala Ala Ile Trp Ser Met Gly Ser Asp Tyr Ser Leu Ala Leu 435 440 445 Ser Leu Ala His Thr Glu Arg Ala Pro Ser Val Gln Glu Leu Tyr Ala 450 455 460 Tyr Gly Leu His Leu Ala Thr Asn Thr Tyr Glu Ile Gly Ile Val Ser 465 470 475 480 Gly Asn Ser Arg Leu Ala Glu Lys Val Ser Gln Gly Val Glu Lys Ala 485 490 495 Asn Ser Val Asn Leu Thr Leu Arg Lys Thr Ala Gly Pro Thr Thr Phe 500 505 510 Thr Ile Gly Ala Tyr His Gln Asp Phe Asp Asn Tyr Ile Tyr Ala Gln 515 520 525 Thr Leu Asp Gln Phe Glu Asp Phe Arg Leu Ile Arg Tyr Ala Gly Ala 530 535 540 Glu Ala Thr Phe Thr Gly Ile Asp Gly Glu Val Arg His Ala Phe Asn 545 550 555 560 Asp Gln Phe Ala Leu Ser Val Phe Gly Asp Tyr Val Arg Ala Lys Leu 565 570 575 Lys Asn Gly Gly Gly Asp Leu Pro Arg Ile Pro Ala Gly Arg Leu Gly 580 585 590 Ala Arg Gly Asp Ala His Val Gly Pro Phe Thr Ala Asp Ala Glu Tyr 595 600 605 Tyr His Val Phe Glu Gln Asp Arg Ile Ala Ala Phe Glu Thr Arg Thr 610 615 620 Pro Gly Tyr Asp Met Val Asn Ala Thr Leu Ala Tyr Arg Leu Glu Leu 625 630 635 640 Gly Asp Lys Arg Ser Ala Glu Leu Phe Val Arg Gly Thr Asn Leu Thr 645 650 655 Asn Glu Leu Ala Tyr Asn His Ser Ser Phe Ile Lys Ala Phe Ser Pro 660 665 670 Leu Arg Gly Arg Asn Phe Val Phe Gly Leu Arg Gly Ala Phe 675 680 685 50921PRTNeisseria meningitidis MC58 50Met Arg Ser Ser Phe Arg Leu Lys Pro Ile Cys Phe Tyr Leu Met Gly 1 5 10 15 Val Thr Leu Tyr His Tyr Ser Tyr Ala Glu Asp Ala Gly Arg Ala Gly 20 25 30 Ser Glu Ala Gln Ile Gln Val Leu Glu Asp Val His Val Lys Ala Lys 35 40 45 Arg Val Pro Lys Asp Lys Lys Val Phe Thr Asp Ala Arg Ala Val Ser 50 55 60 Thr Arg Gln Asp Ile Phe Lys Ser Ser Glu Asn Leu Asp Asn Ile Val 65 70 75 80 Arg Ser Ile Pro Gly Ala Phe Thr Gln Gln Asp Lys Ser Ser Gly Ile 85 90 95 Val Ser Leu Asn Ile Arg Gly Asp Ser Gly Phe Gly Arg Val Asn Thr 100 105 110 Met Val Asp Gly Ile Thr Gln Thr Phe Tyr Ser Thr Ser Thr Asp Ala 115 120 125 Gly Arg Ala Gly Gly Ser Ser Gln Phe Gly Ala Ser Val Asp Ser Asn 130 135 140 Phe Ile Ala Gly Leu Asp Val Val Lys Gly Ser Phe Ser Gly Ser Ala 145

150 155 160 Gly Ile Asn Ser Leu Ala Gly Ser Ala Asn Leu Arg Thr Leu Gly Val 165 170 175 Asp Asp Val Val Gln Gly Asn Asn Thr Tyr Gly Leu Leu Leu Lys Gly 180 185 190 Leu Thr Gly Thr Asn Ser Thr Lys Gly Asn Ala Met Ala Ala Ile Gly 195 200 205 Ala Arg Lys Trp Leu Glu Ser Gly Ala Ser Val Gly Val Leu Tyr Gly 210 215 220 His Ser Arg Arg Ser Val Ala Gln Asn Tyr Arg Val Gly Gly Gly Gly 225 230 235 240 Gln His Ile Gly Asn Phe Gly Ala Glu Tyr Leu Glu Arg Arg Lys Gln 245 250 255 Arg Tyr Phe Val Gln Glu Gly Ala Leu Lys Phe Asn Ser Asp Ser Gly 260 265 270 Lys Trp Glu Arg Asp Leu Gln Arg Gln Gln Trp Lys Tyr Lys Pro Tyr 275 280 285 Lys Asn Tyr Asn Asn Gln Glu Leu Gln Lys Tyr Ile Glu Glu His Asp 290 295 300 Lys Ser Trp Arg Glu Asn Leu Ala Pro Gln Tyr Asp Ile Thr Pro Ile 305 310 315 320 Asp Pro Ser Ser Leu Lys Gln Gln Ser Ala Gly Asn Leu Phe Lys Leu 325 330 335 Glu Tyr Asp Gly Val Phe Asn Lys Tyr Thr Ala Gln Phe Arg Asp Leu 340 345 350 Asn Thr Lys Ile Gly Ser Arg Lys Ile Ile Asn Arg Asn Tyr Gln Phe 355 360 365 Asn Tyr Gly Leu Ser Leu Asn Pro Tyr Thr Asn Leu Asn Leu Thr Ala 370 375 380 Ala Tyr Asn Ser Gly Arg Gln Lys Tyr Pro Lys Gly Ser Lys Phe Thr 385 390 395 400 Gly Trp Gly Leu Leu Lys Asp Phe Glu Thr Tyr Asn Asn Ala Lys Ile 405 410 415 Leu Asp Leu Asn Asn Thr Ala Thr Phe Arg Leu Pro Arg Glu Thr Glu 420 425 430 Leu Gln Thr Thr Leu Gly Phe Asn Tyr Phe His Asn Glu Tyr Gly Lys 435 440 445 Asn Arg Phe Pro Glu Glu Leu Gly Leu Phe Phe Asp Gly Pro Asp Gln 450 455 460 Asp Asn Gly Leu Tyr Ser Tyr Leu Gly Arg Phe Lys Gly Asp Lys Gly 465 470 475 480 Leu Leu Pro Gln Lys Ser Thr Ile Val Gln Pro Ala Gly Ser Gln Tyr 485 490 495 Phe Asn Thr Phe Tyr Phe Asp Ala Ala Leu Lys Lys Asp Ile Tyr Arg 500 505 510 Leu Asn Tyr Ser Thr Asn Thr Val Gly Tyr Arg Phe Gly Gly Glu Tyr 515 520 525 Thr Gly Tyr Tyr Gly Ser Asp Asp Glu Phe Lys Arg Ala Phe Gly Glu 530 535 540 Asn Ser Pro Thr Tyr Lys Lys His Cys Asn Arg Ser Cys Gly Ile Tyr 545 550 555 560 Glu Pro Val Leu Lys Lys Tyr Gly Lys Lys Arg Ala Asn Asn His Ser 565 570 575 Val Ser Ile Ser Ala Asp Phe Gly Asp Tyr Phe Met Pro Phe Ala Ser 580 585 590 Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu Met Tyr Phe Ser 595 600 605 Gln Ile Gly Asp Ser Gly Val His Thr Ala Leu Lys Pro Glu Arg Ala 610 615 620 Asn Thr Trp Gln Phe Gly Phe Asn Thr Tyr Lys Lys Gly Leu Leu Lys 625 630 635 640 Gln Asp Asp Thr Leu Gly Leu Lys Leu Val Gly Tyr Arg Ser Arg Ile 645 650 655 Asp Asn Tyr Ile His Asn Val Tyr Gly Lys Trp Trp Asp Leu Asn Gly 660 665 670 Asp Ile Pro Ser Trp Val Ser Ser Thr Gly Leu Ala Tyr Thr Ile Gln 675 680 685 His Arg Asn Phe Lys Asp Lys Val His Lys His Gly Phe Glu Leu Glu 690 695 700 Leu Asn Tyr Asp Tyr Gly Arg Phe Phe Thr Asn Leu Ser Tyr Ala Tyr 705 710 715 720 Gln Lys Ser Thr Gln Pro Thr Asn Phe Ser Asp Ala Ser Glu Ser Pro 725 730 735 Asn Asn Ala Ser Lys Glu Asp Gln Leu Lys Gln Gly Tyr Gly Leu Ser 740 745 750 Arg Val Ser Ala Leu Pro Arg Asp Tyr Gly Arg Leu Glu Val Gly Thr 755 760 765 Arg Trp Leu Gly Asn Lys Leu Thr Leu Gly Gly Ala Met Arg Tyr Phe 770 775 780 Gly Lys Ser Ile Arg Ala Thr Ala Glu Glu Arg Tyr Ile Asp Gly Thr 785 790 795 800 Asn Gly Gly Asn Thr Ser Asn Phe Arg Gln Leu Gly Lys Arg Ser Ile 805 810 815 Lys Gln Thr Glu Thr Leu Ala Arg Gln Pro Leu Ile Phe Asp Phe Tyr 820 825 830 Ala Ala Tyr Glu Pro Lys Lys Asn Leu Ile Phe Arg Ala Glu Val Lys 835 840 845 Asn Leu Phe Asp Arg Arg Tyr Ile Asp Pro Leu Asp Ala Gly Asn Asp 850 855 860 Ala Ala Thr Gln Arg Tyr Tyr Ser Ser Phe Asp Pro Lys Asp Lys Asp 865 870 875 880 Glu Asp Val Thr Cys Asn Ala Asp Lys Thr Leu Cys Asn Gly Lys Tyr 885 890 895 Gly Gly Thr Ser Lys Ser Val Leu Thr Asn Phe Ala Arg Gly Arg Thr 900 905 910 Phe Leu Met Thr Met Ser Tyr Lys Phe 915 920 51530PRTAggregatibacter actinomycetemcomitans 51Met Val Thr Phe Cys Ile Leu Asn Val Leu Asn Val Ala Asn Ala Glu 1 5 10 15 Glu Gln Leu Asp Gln Ile Asp Val Val Glu Lys Thr Ile Ala Asn Glu 20 25 30 Lys Lys Pro Phe Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val 35 40 45 Phe Lys Ser Thr Glu Thr Val Asp Asn Val Val Arg Ser Ile Pro Gly 50 55 60 Ala Phe Thr Gln Gln Asp Lys Gly Ser Gly Val Leu Ser Leu Asn Ile 65 70 75 80 Arg Gly Glu Thr Gly Phe Gly Arg Ala Asn Thr Met Val Asp Gly Val 85 90 95 Thr Gln Thr Phe Tyr Ser Thr Ser Met Asp Ala Gly Arg Thr Gly Gly 100 105 110 Asn Ser Gln Phe Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Ile 115 120 125 Asp Leu Thr Lys Gly Asn Phe Ser Gly Thr Gly Gly Val Asn Ser Leu 130 135 140 Tyr Gly Ala Ala Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Gln 145 150 155 160 Gly Asp Lys Asn Tyr Gly Phe Leu Thr Lys Gly Leu Ser Gly Asp Asn 165 170 175 Glu Thr Lys Tyr Asn Tyr Met Ala Met Gly Ala Ala Arg Lys Trp Leu 180 185 190 Asp Asn Gly Gly Tyr Ile Gly Val Leu Tyr Gly Tyr Ser Gln Arg Glu 195 200 205 Val Ser Gln Asn Tyr Lys Val Gly Gly Gly Gly Glu Arg Ile Ser Asp 210 215 220 Val Gly Lys Gly Phe Leu Glu Arg Lys Arg Asn Glu Phe Phe Arg Ser 225 230 235 240 His Gln Leu Lys Phe Asn Ser Glu Lys Asn Glu Trp Glu Arg Asp Phe 245 250 255 Ser Ile Lys Asn Ser Ala Gly Lys Ser Thr Trp Glu Tyr Pro Trp Asn 260 265 270 Lys Lys Tyr Asn Asp Pro Gln Lys Leu Lys Glu Tyr Ile Ala Glu Leu 275 280 285 Gly Lys Ile Trp Asn Glu Asn Glu Val Pro Gln Trp Asp Leu Thr Pro 290 295 300 Ile Asp Pro Ser Ser Leu Val Gln Arg Ser Lys Ser His Leu Val Lys 305 310 315 320 Val Glu Phe Ser Asp Asp Arg His Thr Leu Asn Leu Gln Tyr Arg Thr 325 330 335 Leu Asp Asn His Ile Gly Ser Arg Lys Ile Glu Asn Arg Asn Tyr Gln 340 345 350 Leu Asn Tyr Asn Phe Asn Asn Asn Gly Tyr Leu Asp Leu Asn Val Leu 355 360 365 Leu Ala His Asn Val Gly Lys Gln Lys Tyr Pro Ser Gly Ser Arg Phe 370 375 380 Gly Gly Trp Gln Val Leu Lys Tyr Leu Glu Thr Lys Asn Thr Ala Asp 385 390 395 400 Ile Ile Asp Ile Ser Asn Ser Tyr Thr Phe Ser Leu Pro Lys Glu Thr 405 410 415 Asp Leu Lys Thr Thr Leu Gly Val Asn Leu Phe Lys Asn Gln Tyr Thr 420 425 430 Lys Asn Arg Phe Pro Glu Glu Leu Ser Pro Phe Tyr Asp Gly Pro Ser 435 440 445 Gln Lys Ser Gly Leu Tyr Asp Phe Leu Gly Arg Phe Lys Gly Asp Lys 450 455 460 Gly Ile Leu Pro Gln Lys Ser Thr Ile Leu Gln Pro Ser Gly Glu Gln 465 470 475 480 Arg Phe Asn Thr Val Tyr Leu Asp Thr Ser Leu Thr Arg Asp Ile Phe 485 490 495 Lys Leu Asp Tyr Ser Val Asn Phe Val Lys Tyr Lys Phe Asn Gly Glu 500 505 510 Tyr Thr Ala Tyr Tyr Asn Ser Pro Ser Asp Phe Lys Lys Ala Phe Gly 515 520 525 Glu Asp 530 52365PRTAggregatibacter actinomycetemcomitans D11S-1 52Met Tyr Glu Pro Val Tyr Lys Lys Gly Gly Lys Lys Ser Ala Val Asn 1 5 10 15 His Ser Val Val Leu Ser Ala Ala Pro Ser Asp Tyr Phe Met Pro Phe 20 25 30 Ile Ser Tyr Ala Arg Thr His Arg Met Pro Asn Ile Gln Glu Met Tyr 35 40 45 Phe Ser Gln Ile Gly Asp Ser Gly Val Asn Thr Asn Leu Lys Pro Glu 50 55 60 Arg Ala Asp Thr Tyr Gln Ile Gly Phe Asn Thr Phe Lys Asn Asn Val 65 70 75 80 Phe Leu Asp Asp Asp Val Leu Gly Leu Lys Ala Val Phe Tyr Arg Ser 85 90 95 Arg Ile Lys Asp Tyr Ile His Asn Val Tyr Gly Lys Trp Trp Asn Thr 100 105 110 Gln Ala Gly Leu Pro Pro Ser Trp Val Thr Thr Thr Gly Leu Ser Tyr 115 120 125 Thr Ile Gln His Arg Asn Tyr Gln Lys Arg Val Asn Lys Arg Gly Leu 130 135 140 Glu Leu Glu Leu Asn Tyr Asp Ala Gly Arg Phe Phe Thr Asn Leu Ser 145 150 155 160 Tyr Ala Tyr Gln Lys Thr Asn Gln Pro Thr Asn Tyr Ser Asp Ala Ser 165 170 175 Glu Ser Pro Asn Asn Ala Ser Lys Gln Asp Gln Ile Lys Gln Gly Tyr 180 185 190 Gly Leu Thr Lys Ile Ser Met Leu Pro Arg Asp Tyr Gly Arg Leu Glu 195 200 205 Ile Gly Ser Arg Trp Phe Asp Arg Lys Leu Thr Ile Gly Ser Ala Val 210 215 220 Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Thr Glu Glu Arg Tyr Ile 225 230 235 240 Asp Gly Thr Lys Pro Gly Asn Thr Ala Asp Pro His Asn Ile Gly Lys 245 250 255 Arg Val Ile Lys Glu Thr Glu Thr Ile Asp Lys Gln Pro Leu Val Val 260 265 270 Asp Phe Tyr Val Ala Tyr Glu Pro Val Glu Asn Leu Val Ile Arg Ala 275 280 285 Asp Ile Gln Asn Ala Phe Asp Lys Arg Tyr Ile Asp Pro Leu Asp Ala 290 295 300 Ala Asn Asp Ala Ala Thr Gln Arg Tyr Phe Ser Thr Phe Glu Asn Leu 305 310 315 320 Asn Ser Tyr Gly Asp Asp Ile Val Gln Cys Asp Ser Asn Gly Leu Cys 325 330 335 Asn Gly Arg Tyr Gly Gly Lys Thr Asn Ser Ile Leu Asn Asn Tyr Ala 340 345 350 Arg Gly Arg Thr Phe Val Leu Ser Val Ser Tyr Lys Phe 355 360 365 53924PRTAggregatibacter aphrophilus NJ8700 53Met Arg Lys Thr Ile Lys Leu Asn Leu Ile Thr Phe Cys Leu Leu Asn 1 5 10 15 Val Ile Asn Ile Thr Tyr Ala Glu Glu Gln Leu Asn Gln Ile Asp Val 20 25 30 Val Glu Lys Ile Ile Ala Asn Glu Lys Lys Pro Phe Thr Glu Ala Lys 35 40 45 Ala Lys Ser Thr Arg Glu His Val Phe Lys Glu Thr Gln Ser Ile Asp 50 55 60 Asn Val Val Arg Ser Ile Pro Gly Ala Phe Thr Gln Gln Asp Lys Gly 65 70 75 80 Ser Gly Val Leu Ser Leu Asn Ile Arg Gly Glu Thr Gly Phe Gly Arg 85 90 95 Ala Asn Thr Met Val Asp Gly Ile Thr Gln Thr Phe Tyr Ser Thr Ser 100 105 110 Met Asp Ser Gly Gln Ala Gly Gly Asn Ser Gln Phe Gly Ala Ser Leu 115 120 125 Asp Pro Asn Phe Ile Ala Gly Ile Asp Leu Thr Lys Gly Asn Phe Ser 130 135 140 Gly Thr Asn Gly Val Asn Ser Leu Tyr Gly Ser Ala Asn Phe Arg Thr 145 150 155 160 Leu Gly Val Asn Asp Val Ile Leu Gly Asp Lys Asn Leu Gly Phe Ile 165 170 175 Ile Lys Gly Met Thr Gly Thr Asn Ala Thr Lys Ser Asn Tyr Met Ala 180 185 190 Met Ala Ala Thr Arg Lys Trp Leu Asp Asn Gly Gly Tyr Val Gly Met 195 200 205 Leu Tyr Gly Tyr Ser Arg Arg Glu Val Ser Gln Asp Tyr Lys Ile Gly 210 215 220 Gly Gly Gly Lys Lys Ile Ala Asp Val Gly Asp Asp Phe Leu Gln Gln 225 230 235 240 Gln Lys Asn Lys Gln Phe Thr Glu Ala Gly Phe Val Phe Asp Arg Ala 245 250 255 Lys Gly Arg Trp Met Pro Asp Leu Asn Lys Asn Leu Trp Ala Cys Asn 260 265 270 Ala Pro Val Pro Ser Gln Pro Glu Pro Tyr Asp Cys Lys Tyr Tyr Lys 275 280 285 Asn Ala Glu Arg Lys Arg Ile Leu Glu Asp Pro Asp Asn Ser Pro Glu 290 295 300 Leu Gln Lys Gln Ile Asp Glu Thr Asn Ala Ala Phe Glu Arg Asn Asn 305 310 315 320 Glu Gln Tyr Arg Leu Ala Pro Leu Asp Pro Ser Ser Val Arg Gln Ser 325 330 335 Ser His Ser His Leu Val Lys Leu Glu Tyr Gly Asp Asp Arg His Thr 340 345 350 Leu Asn Leu Gln Leu Arg Ala Leu Asp Asn Ala Ile Gly Thr Arg Lys 355 360 365 Ile Glu Asn Arg Asn Tyr Gln Leu Asn Tyr Asn Phe Asn Asn Asn Gly 370 375 380 Tyr Ile Asp Met Asn Leu Leu Leu Ala His Asn Val Gly Lys Ser Lys 385 390 395 400 Tyr Pro Lys Gly Ser Arg Phe Thr Gly Trp Glu Val Leu Lys Tyr Leu 405 410 415 Glu Thr Lys Asn Ala Ala Asp Ile Phe Asp Ile Ser Asn Ser Tyr Thr 420 425 430 Phe Thr Leu Pro Lys Glu Val Asp Leu Lys Thr Thr Leu Gly Phe Asn 435 440 445 Phe Phe Lys Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu Leu Ser 450 455 460 Leu Phe Tyr Asp Gly Ser Ser Gln Arg Gly Gly Leu Tyr Asp Phe Leu 465 470 475 480 Gly Arg Tyr Lys Gly Ser Lys Gly Leu Leu Pro Gln Lys Ser Ser Ile 485 490 495 Leu His Pro Ser Gly Glu Gln Lys Phe His Thr Phe Tyr Leu Asp Thr 500 505 510 Ser Leu Thr Arg Asp Ile Tyr Arg Leu Asp Tyr Ser Val Asn Phe Ile 515 520 525 Lys Tyr Lys Phe Asn Gly Glu Tyr Ala Gly Tyr Tyr Asn Ser Pro Glu 530 535 540 Asp Phe Glu Lys Ala Phe Gly Lys Asp Ser Gln Ile Tyr Lys Lys Tyr 545 550 555 560 Cys Lys Pro Asn Gly Gly Cys Gln Ile Tyr Glu Pro Leu Glu Arg Lys 565 570 575 Thr Gly His Lys Thr Ala Val Asn His Ser Ala Ile Phe Ser Ala His 580 585 590 Leu His Asp Tyr Phe Met Pro Phe Met Gly Tyr Ala Arg Thr His Arg 595 600 605 Met Pro Asn Ile Gln Glu Met Tyr Phe Ser Gln Val Ser Asp Ala Gly 610 615 620 Val Asn Thr Asp

Leu Lys Pro Glu Arg Ala Ser Thr Tyr Gln Leu Gly 625 630 635 640 Phe Asn Thr Phe Lys Glu Gly Ile Trp Lys Asn Asp Asp Val Leu Gly 645 650 655 Ile Lys Val Val Gly Tyr Arg Ser Tyr Ile Lys Asn Tyr Ile His Asn 660 665 670 Val Trp Gly Glu Trp Trp Gln Gly Gly Ala Pro Thr Trp Ala Glu Ser 675 680 685 Asn Gly Phe Gln Phe Thr Ile Ala His Arg Asn Tyr Ala Lys Thr Val 690 695 700 Lys Lys Ser Gly Val Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg Phe 705 710 715 720 Phe Thr Asn Leu Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn 725 730 735 Tyr Ser Asp Ala Ser Pro Arg Pro Asn Asn Ala Ser Lys Glu Asp Val 740 745 750 Leu Lys Gln Gly Tyr Gly Leu Thr Arg Ile Ser Ala Leu Pro Arg Asp 755 760 765 Tyr Gly Arg Leu Glu Leu Gly Ser Arg Trp Phe Asp Arg Lys Leu Thr 770 775 780 Val Gly Gly Ala Val Arg Tyr Tyr Gly Glu Ser Lys Arg Ala Ser Ile 785 790 795 800 Glu Glu Lys Tyr Ile Asp Gly Thr Lys Phe Glu Lys Asn Ala Leu Arg 805 810 815 Arg His His His Ala Ile Lys Glu Thr Glu Thr Ile Glu Lys Gln Pro 820 825 830 Leu Ile Phe Asp Leu Tyr Val Ser Tyr Glu Pro Ile Glu Asn Leu Ile 835 840 845 Leu Lys Thr Glu Val Gln Asn Leu Phe Asp Lys Lys Tyr Ile Asp Pro 850 855 860 Leu Asp Ala Gly Asn Asp Ala Ala Thr Gln Arg Tyr Tyr Ser Ser Ile 865 870 875 880 Ser Ser Ala Pro Ser Gln Pro Cys Ala Pro Gly Glu Leu Cys His Lys 885 890 895 Asp Gly His Ser Gly Lys Thr Gln Ser Ile Leu Asn Asn Tyr Ala Arg 900 905 910 Gly Arg Thr Ile Val Phe Ser Leu Ser Tyr Lys Phe 915 920 54758PRTCampylobacter coli RM2228 54Met Lys Lys Leu Ser Leu Phe Cys Ala Val Gly Leu Cys Phe Ala Asn 1 5 10 15 Phe Ala Phe Ser Glu Glu Leu Glu Phe Asp Ser Leu Glu Ile Ser Gly 20 25 30 Ser Lys Ile Lys Asn Asp Glu Lys Pro Phe Val Thr Pro Gly Ala Thr 35 40 45 Ser Thr Arg Asp Asn Leu Asn Ser Asn Thr Gln Ser Ile Asp Ser Ile 50 55 60 Val Arg Ser Val Pro Gly Ala Tyr Thr Gln Val Asp Gln Ala Gln Gly 65 70 75 80 Gly Val Ser Val Asn Ile Arg Gly Met Thr Gly Leu Gly Arg Val Asn 85 90 95 Thr Gln Ile Asp Gly Val Thr Gln Thr Phe Phe Gly Ser Ala Ser Asp 100 105 110 Asp Ser Phe His Gly Gln Ile Gly Thr Ser Ser Phe Thr Ala Pro Ile 115 120 125 Asp Lys Asn Phe Leu Val Ala Leu Asp Val Asn Arg Gly Thr Phe Ser 130 135 140 Gly Ala Asn Gly Ala Asn Ala Leu Met Gly Ser Ala Asn Phe Arg Thr 145 150 155 160 Ile Gly Val Asp Asp Ile Val Gln Glu Gly Arg Val Phe Gly Phe Leu 165 170 175 Gly Lys Tyr Ser Tyr Gly Ser Asn Ala Ile Gly Pro Asn Phe Met Gly 180 185 190 Ala Ile Ala Gly Lys Thr Met Leu Glu Asn Asn Ala Asn Leu Gly Ile 195 200 205 Leu Phe Ala Tyr Ser Gly Gln Asn Val Ser Gln Asn Tyr Lys Ile Gly 210 215 220 Gly Gly Gly Tyr Ile Gly Asp Gln Lys Pro Pro Phe Asp Thr Asp Asp 225 230 235 240 Asp Gly Ile Pro Asp Asp Asn Leu Ala Ala Pro Ile Asn Pro Asn Asn 245 250 255 Leu Tyr Asn Gln Pro Arg Ser Gln Leu Phe Lys Val Glu Tyr Lys Ser 260 265 270 Asp Ile Ser Glu Ala Ile Leu Asn Tyr Arg Ala Tyr Lys Asn Asn Leu 275 280 285 Ala Gly Arg Lys Ile Thr Asn Asp Thr Tyr Gln Ile Asp Tyr Arg Leu 290 295 300 Asn Pro Asp Ser Asn Leu Leu Asp Leu Lys Phe Leu Phe Ala Tyr Asn 305 310 315 320 Asp Gly Lys Gln Lys Tyr Asn Gln Gly Ser Thr Trp Gly Tyr His Asp 325 330 335 Met Ser Gly Val Lys Thr Lys Asn Arg Ala Val Thr Phe Asp Leu Ser 340 345 350 Asn Thr Met Asn Lys Glu Phe Ser Gln Asp Ser Asn Leu Tyr Phe Thr 355 360 365 Tyr Gly Val Asn Ile Leu Asn Asn His Tyr Ser Asn Asp Phe Pro Gln 370 375 380 Asp Arg Val Leu Leu Pro Tyr Ile Leu Thr Ser Phe Tyr Pro Lys Gly 385 390 395 400 Lys Gln Asp Ile Lys Thr Leu Tyr Leu Asp Thr Ser Phe Thr Lys Gly 405 410 415 Ile Phe Thr Leu Asn Ser Asn Ile Asn Trp Thr Asn Ala Gln Leu Ser 420 425 430 Gly Tyr Lys Gly Ile Cys Ser Met Ala Asn Pro Tyr Cys Gln Pro Lys 435 440 445 Asn Ala Thr Asn Leu Glu Lys Asp Tyr Asn Asn Phe Asn Tyr Ser Leu 450 455 460 Met Leu Ser Ala Asp Ile His Pro Leu Phe Asn Pro Phe Ile Ser Tyr 465 470 475 480 Ser Lys Ser His Arg Ile Pro Asn Val Gln Glu Tyr Phe Phe Thr His 485 490 495 Asp Ala Ser Phe Glu His Asn Met Asn Thr Phe Leu Lys Ala Glu Ser 500 505 510 Ala Asp Thr Tyr Gln Ile Gly Phe Asn Ser Phe Thr His Glu Ile Leu 515 520 525 Asn Asp Ser Asp Thr Leu Gly Phe Lys Met Leu Tyr Tyr Asp Thr Lys 530 535 540 Val Lys Asn Tyr Ile Tyr Asn Arg Arg Tyr Trp Gln Lys Ala Asp Asp 545 550 555 560 Ala Ile Phe Leu Met Gln Leu Asn Asp Asp Glu Lys Ala Lys Phe His 565 570 575 Gly Val Glu Leu Glu Phe Lys Tyr Asp Thr Gly Phe Phe Tyr Ser Ile 580 585 590 Leu Ser Tyr Thr Tyr Gln Lys Ser Lys His Lys Phe Ser Asp Thr Glu 595 600 605 Ser Leu Glu Phe Gly Gly Ala Gln Ser Gly Gln Ser Gln Phe Ala Gln 610 615 620 Leu Pro Glu His Tyr Ala Asn Leu Asp Met Gly Val Arg Leu Phe Glu 625 630 635 640 Glu Lys Leu Thr Leu Gly Ala Leu Ala Lys Tyr Thr Gly Lys Ala Lys 645 650 655 Arg Ile Val Pro Val Gly Ser Leu Asp Asp Asp Pro Ser Asn Pro Asp 660 665 670 Ala Met Ala Pro Leu Lys Thr Thr Asp Glu Leu Pro Lys Ile Pro Thr 675 680 685 Ile Val Asp Leu Tyr Ala Asn Tyr Lys Ile Leu Lys Asn Phe Thr Ile 690 695 700 Lys Ala Glu Val Gln Asn Leu Phe Asp Lys Asn Tyr Met Asp Ala Leu 705 710 715 720 Tyr Ser Tyr Asn Thr Gly Glu Asn Gln Asn Ala Gly Gly Leu Phe Asp 725 730 735 Pro Ile Tyr Ile Tyr Asn Asn Ser Ala Arg Gly Arg Thr Phe Ile Val 740 745 750 Ser Phe Glu Tyr Lys Tyr 755 55758PRTCampylobacter upsaliensis RM3195 55Met Lys Lys Leu Ser Leu Phe Cys Val Met Gly Leu Tyr Phe Ala Asn 1 5 10 15 Phe Ala Phe Ser Glu Glu Leu Glu Phe Asp Ser Leu Glu Ile Ser Gly 20 25 30 Ser Lys Ile Lys Asn Asp Glu Lys Pro Phe Val Thr Pro Gly Ala Thr 35 40 45 Ser Thr Arg Asp Asn Leu Asn Ser Ser Thr Gln Ser Ile Asp Ser Ile 50 55 60 Val Arg Ser Val Pro Gly Ala Tyr Thr Gln Val Asp Gln Ala Gln Gly 65 70 75 80 Gly Val Ser Val Asn Ile Arg Gly Met Thr Gly Leu Gly Arg Val Asn 85 90 95 Thr Gln Ile Asp Gly Val Thr Gln Thr Phe Phe Gly Ser Ala Ser Asp 100 105 110 Asp Ser Phe His Gly Gln Ile Gly Thr Ser Ser Phe Thr Ala Pro Ile 115 120 125 Asp Lys Asn Phe Leu Val Ala Leu Asp Val Asn Arg Gly Thr Phe Ser 130 135 140 Gly Ala Asn Gly Ala Asn Ala Leu Met Gly Ser Ala Asn Phe Arg Thr 145 150 155 160 Ile Gly Val Asp Asp Ile Val Gln Glu Gly Arg Val Phe Gly Phe Leu 165 170 175 Gly Lys Tyr Ser Tyr Gly Ser Asn Ala Ile Gly Pro Asn Phe Met Gly 180 185 190 Ala Ile Ala Gly Lys Thr Met Leu Glu Asn Asn Ala Asn Leu Gly Ile 195 200 205 Leu Phe Ala Tyr Ser Gly Gln Asn Val Ser Gln Asn Tyr Lys Ile Gly 210 215 220 Gly Gly Gly Tyr Ile Gly Asp Gln Lys Pro Pro Phe Asp Thr Asp Asp 225 230 235 240 Asp Gly Thr Pro Asp Asp Asn Leu Ala Ala Pro Ile Asn Pro Asn Asn 245 250 255 Leu Tyr Asn Gln Pro Arg Ser Gln Leu Phe Lys Val Glu Tyr Lys Ser 260 265 270 Asp Ile Ser Glu Ala Ile Leu Asn Tyr Arg Ala Tyr Lys Asn Asn Leu 275 280 285 Ala Gly Arg Lys Ile Thr Asn Asp Thr Tyr Gln Ile Asp Tyr Arg Leu 290 295 300 Asn Pro Asp Ser Asn Leu Leu Asp Leu Lys Phe Leu Phe Ala Tyr Asn 305 310 315 320 Asp Gly Lys Gln Lys Tyr Asn Gln Asp Lys Thr Trp Gly Tyr His Asn 325 330 335 Met Ser Gly Ile Lys Thr Lys Asn Arg Ala Val Thr Phe Asp Leu Ser 340 345 350 Asn Thr Met Asn Lys Glu Phe Ser Gln Asp Ser Asn Leu Tyr Phe Thr 355 360 365 Tyr Gly Val Asn Ile Leu Asn Asn His Tyr Ser Asn Asp Phe Pro Glu 370 375 380 Asp Arg Val Leu Leu Pro Tyr Ile Leu Thr Ser Phe Tyr Pro Lys Gly 385 390 395 400 Lys Gln Glu Ile Lys Thr Phe Tyr Leu Asp Thr Ser Phe Thr Lys Gly 405 410 415 Ile Phe Thr Leu Asn Ser Asn Val Asn Trp Ile Asn Ala Gln Leu Ser 420 425 430 Gly Tyr Lys Gly Val Cys Ser Met Val Asn Pro Tyr Cys Gln Pro Lys 435 440 445 Asn Ala Thr Asn Leu Glu Lys Asp Tyr Asn Asn Phe Asn Tyr Ser Leu 450 455 460 Met Leu Ser Ala Asp Ile His Pro Leu Phe Asn Pro Phe Ile Ser Tyr 465 470 475 480 Ser Lys Ser His Arg Ile Pro Asn Val Gln Glu Tyr Phe Phe Thr His 485 490 495 Asp Ala Ser Gly Glu His Asn Met Asn Thr Phe Leu Arg Ala Glu Ser 500 505 510 Ala Asn Thr Tyr Gln Ile Gly Phe Asn Ser Phe Ala His Glu Ile Leu 515 520 525 Asn Asn Asn Asp Thr Leu Gly Phe Lys Val Leu Tyr Tyr Asp Thr Lys 530 535 540 Val Lys Asn Tyr Ile Tyr Asn Arg Arg Tyr Trp Leu Lys Ala Asp Lys 545 550 555 560 Ala Ile Phe Ile Met Gln Leu Asn Asp Asp Glu Lys Ala Lys Phe His 565 570 575 Gly Val Glu Leu Glu Phe Lys Tyr Asp Thr Gly Phe Phe Tyr Ser Ile 580 585 590 Leu Ser Tyr Thr Tyr Gln Lys Ser Lys His Lys Phe Ser Asp Thr Glu 595 600 605 Ser Leu Glu Thr Gly Gly Ala Gln Ser Gly Gln Ser Gln Phe Ala Gln 610 615 620 Leu Pro Glu His Tyr Ala Asn Leu Asp Met Gly Val Arg Leu Phe Glu 625 630 635 640 Glu Lys Leu Thr Leu Gly Ala Leu Ala Lys Tyr Thr Gly Lys Ala Lys 645 650 655 Arg Ile Ala Pro Val Gly Ser Leu Glu Asp Asp Pro Ser Asn Pro Asp 660 665 670 Ala Met Ala Pro Leu Lys Thr Thr Asp Lys Leu Pro Lys Ile Pro Thr 675 680 685 Ile Val Asp Leu Tyr Ala Asn Tyr Lys Ile Leu Lys Asn Phe Thr Ile 690 695 700 Lys Ala Glu Ile Gln Asn Leu Phe Asp Lys Asn Tyr Met Asp Ala Leu 705 710 715 720 Tyr Thr Tyr Asn Thr Gly Asp Ser Gln Asn Val Gly Gly Leu Phe Asn 725 730 735 Pro Ile Tyr Ile Tyr Asn Asn Ser Ala Arg Gly Arg Thr Phe Ile Val 740 745 750 Ser Phe Glu Tyr Lys Tyr 755 56921PRTHaemophilus influenzae 3655 56Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Ser Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Thr Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Val 225 230 235 240 Leu Ala Lys Glu Lys Glu Lys Ile Phe Arg Asn Asp Gly Tyr Val Leu 245 250 255 Asn Ser Ala Gly Gln Trp Ala Pro Asp Leu Ser Gln Asn Ser Trp Thr 260 265 270 Cys Asn Ala Lys Lys Pro Tyr Leu Ala Asp Glu Arg Val Thr Glu Gly 275 280 285 Phe Lys Pro Asp Cys Lys Phe Ile Ala Phe Pro Lys Ser Pro Thr Thr 290 295 300 Ile Lys Arg Lys Lys Ile Leu Gln Asp Ile Asp Asp Gly Lys Pro Leu 305 310 315 320 Gln Asp Ile Pro Glu Leu Gln Ala Asp Ile Lys Glu Thr Asp Asp Ser 325 330 335 Phe Glu Arg Asn Lys Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly 340 345 350 Ser Leu Gln Ser Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Gly 355 360 365 Asp Asp His His Thr Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys 370 375 380 Ile Gly Ser Arg Lys Ile Glu Asn Arg Asn Tyr Gln Ala Asn Tyr Asn 385 390 395 400 Phe Asn Asn Asn Ser Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn 405 410 415 Ile Gly Lys Thr Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Gln 420 425 430 Val Ala Asp Lys Leu Ile Ala Lys Asn Val Ala Asn Ile Val Asp Ile 435 440 445 Asn Asn Ser His Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr 450 455 460 Thr Leu Gly Phe Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe 465 470

475 480 Pro Glu Glu Leu Ser Leu Phe Tyr Val Asn Glu Ser His Asp Gln Gly 485 490 495 Leu Tyr Ser Leu Ser Asn Lys Gly Arg Tyr Ser Gly Ser Lys Gly Leu 500 505 510 Leu Pro Gln Arg Ser Val Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe 515 520 525 Lys Thr Val Tyr Phe Asp Thr Ala Leu Ser Lys Gly Ile Tyr His Leu 530 535 540 Asn Tyr Ser Val Asn Phe Thr His Tyr Ala Phe Asn Gly Glu Tyr Val 545 550 555 560 Gly Tyr Lys Asn Thr Ala Asp Lys Ile Asn Glu Pro Ile Leu His Lys 565 570 575 Ser Gly His Lys Lys Ala Phe Asn His Ser Ala Thr Leu Ser Ala Glu 580 585 590 Leu Ser Asp Tyr Phe Met Pro Phe Phe Thr Tyr Ser Arg Thr His Arg 595 600 605 Met Pro Asn Ile Gln Glu Met Phe Phe Ser Gln Val Ser Asp Ala Gly 610 615 620 Val Asn Thr Ala Leu Lys Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly 625 630 635 640 Phe Asn Thr Tyr Lys Lys Gly Leu Phe Thr Gln Asp Asp Val Leu Gly 645 650 655 Val Lys Leu Val Gly Tyr Arg Ser Phe Ile Lys Asn Tyr Ile His Asn 660 665 670 Val Tyr Gly Asp Trp Ser Arg Asp Gly Val Ile Pro Glu Trp Ala Lys 675 680 685 Leu Asn Gly Phe Arg Leu Thr Ile Ala His Gln Asn Tyr Gln Pro Ile 690 695 700 Val Lys Lys Ser Gly Ala Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg 705 710 715 720 Phe Phe Ala Asn Leu Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr 725 730 735 Asn Tyr Ala Asp Ala Ser Pro Arg Pro Arg Asn Ala Ser Lys Glu Glu 740 745 750 Ile Leu Lys Gln Gly Tyr Gly Leu Ser Arg Ile Thr Met Leu Pro Lys 755 760 765 Asp Tyr Gly Arg Leu Glu Leu Gly Thr Arg Trp Phe Asp Gln Lys Leu 770 775 780 Thr Leu Gly Ile Ala Ala Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr 785 790 795 800 Thr Gln Glu Glu Tyr Ile Asn Gly Ser Arg Tyr Glu Lys Asn Thr Thr 805 810 815 Arg Asp Arg Ile Tyr Tyr Ala Ile Lys Lys Thr Glu Glu Ile Lys Lys 820 825 830 Gln Pro Ile Ile Leu Asp Leu His Val Ser Tyr Glu Pro Ile Lys Asp 835 840 845 Leu Ile Ile Lys Ala Glu Val Gln Asn Leu Leu Asp Lys Arg Tyr Val 850 855 860 Asp Pro Leu Asp Ala Gly Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser 865 870 875 880 Ser Leu Asn Asp Ser Leu Ala Cys Lys Ile Asn Glu Ser Thr Cys Asn 885 890 895 Asp Gly Ser Asp Lys Ser Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr 900 905 910 Tyr Ile Leu Ser Leu Asn Tyr Lys Phe 915 920 57950PRTHaemophilus influenzae 6P18H1 57Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Ser Leu Ile Asn 1 5 10 15 Thr Ile Gly Ile Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Ser Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Thr Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Phe 245 250 255 Thr Asn Gly Gln Trp Thr Pro Asp Leu Asn Lys Asn His Trp Ser Cys 260 265 270 Asn Leu Glu Lys Pro Lys Tyr Ser Gly Ser Gln Asp Pro Ile Val Lys 275 280 285 Thr Asn Glu Leu Thr Gly Glu Thr Glu Thr Ile Trp Thr Asp Ser Asn 290 295 300 Cys Ile Thr Asn Ile Glu Lys Thr Thr Asp Pro Ser Asp Pro Ser Thr 305 310 315 320 Lys Thr Thr Tyr Lys Thr Asn Ile Tyr Lys Asn Asn Thr Arg Gln Asp 325 330 335 Ile Leu Lys Lys Leu Lys Ala Gly Thr Lys Pro Glu Asn Ile Asp Glu 340 345 350 Leu Gln Asn Gly Lys Asp Gly Ile Lys Glu Thr Asp Lys Ser Phe Glu 355 360 365 Asp Asn Lys Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu 370 375 380 Gln Ser Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp 385 390 395 400 His His Thr Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys Ile Gly 405 410 415 Ser Arg Lys Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn 420 425 430 Asn Asn Ser Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly 435 440 445 Lys Thr Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Gln Val Ala 450 455 460 Asp Lys Leu Ile Thr Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn 465 470 475 480 Ser His Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu 485 490 495 Gly Phe Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu 500 505 510 Glu Leu Ser Leu Phe Tyr Asn Asp Ala Ser His Asp Gln Gly Leu Tyr 515 520 525 Ser His Ser Lys Arg Gly Arg Tyr Ser Gly Thr Lys Ser Leu Leu Pro 530 535 540 Gln Arg Ser Val Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr 545 550 555 560 Val Tyr Phe Asp Thr Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr 565 570 575 Ser Val Asn Phe Thr His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr 580 585 590 Glu Asn Thr Thr Asn Lys Asp Asn Glu Pro Ile Leu His Lys Ser Gly 595 600 605 His Lys Lys Ala Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser 610 615 620 Asp Tyr Phe Met Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met Pro 625 630 635 640 Asn Ile Gln Glu Met Phe Phe Ser Gln Val Ser Asn Ala Gly Val Asn 645 650 655 Thr Ala Leu Lys Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn 660 665 670 Thr Tyr Lys Lys Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Val Lys 675 680 685 Leu Val Gly Tyr Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr 690 695 700 Gly Val Trp Trp Arg Asp Gly Val Pro Thr Trp Ala Asp Ser Asn Gly 705 710 715 720 Phe Arg Phe Thr Ile Ala His Gln Asn Tyr Lys Pro Ile Val Lys Lys 725 730 735 Ser Gly Ala Glu Leu Glu Ile Asn Tyr Asp Met Gly Arg Phe Phe Ala 740 745 750 Asn Val Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala 755 760 765 Asp Ala Ser Pro Arg Pro Asn Asn Ala Ser Gln Glu Asp Ile Leu Lys 770 775 780 Gln Gly Tyr Gly Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly 785 790 795 800 Arg Leu Glu Leu Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly 805 810 815 Leu Ala Ala Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu 820 825 830 Glu Tyr Ile Asn Gly Ser His Tyr Glu Gln Lys Thr Ser Gly Ser Arg 835 840 845 Thr Tyr Tyr Ala Val Lys Lys Thr Glu Asp Ile Lys Lys Gln Pro Ile 850 855 860 Ile Leu Asp Leu His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile 865 870 875 880 Lys Ala Glu Val Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu 885 890 895 Asp Ala Gly Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn 900 905 910 Asp Ser Ile Cys Asn Gln Lys Ala Asn Ser Cys Glu Gly Gly Gly Lys 915 920 925 Asp Lys Thr Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu 930 935 940 Ser Leu Asn Tyr Lys Phe 945 950 58915PRTHaemophilus influenzae 7P49H1 58Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Ser Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Ser Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Thr Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Leu 245 250 255 Asn Ser Ala Gly Gln Trp Thr Pro Asp Leu Ser Lys Asn His Trp Ser 260 265 270 Cys Ser Lys Glu Lys Pro Ser Leu Ala Asp Ala Arg Lys Ile Gly Asp 275 280 285 Tyr Thr Pro Lys Cys Gly Asp Phe Ala Pro Pro Arg Asp Pro Thr Thr 290 295 300 Met Lys Arg Lys Lys Ile Leu Lys Asp Ile Asp Ala Lys Lys Pro Leu 305 310 315 320 Gln Glu Ile Gln Glu Leu Gln Ala Asp Ile Asn Lys Thr Asp Lys Ser 325 330 335 Phe Glu Asn Asn Lys Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly 340 345 350 Ser Leu Gln Ser Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Gly 355 360 365 Asp Asp His His Thr Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys 370 375 380 Ile Gly Ser Arg Lys Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn 385 390 395 400 Phe Asn Asn Asn Ser Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn 405 410 415 Ile Gly Lys Thr Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Gln 420 425 430 Val Ala Asp Lys Leu Ile Thr Lys Asn Val Ala Asn Ile Val Asp Ile 435 440 445 Asn Asn Ser His Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr 450 455 460 Thr Leu Gly Phe Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe 465 470 475 480 Pro Glu Glu Leu Ser Leu Phe Tyr Asp Asp Pro Ser His Asp Arg Gly 485 490 495 Asn Tyr Ser Asn Leu Gly Arg Leu Lys Gly Ala Arg Gly Leu Leu Pro 500 505 510 Gln Arg Ser Val Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr 515 520 525 Val Tyr Phe Asp Thr Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr 530 535 540 Ser Val Asn Phe Ile His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr 545 550 555 560 Glu Asn Gly Ala Glu Pro Ile Leu His Lys Ser Gly His Lys Lys Ala 565 570 575 Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe Met 580 585 590 Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu 595 600 605 Met Phe Phe Ser Gln Val Ser Asp Ala Gly Val Asn Thr Ala Leu Lys 610 615 620 Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys Lys 625 630 635 640 Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Val Lys Leu Val Gly Tyr 645 650 655 Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Val Trp Trp 660 665 670 Arg Asp Gly Val Val Pro Thr Trp Ala Asn Ser Asn Gly Phe Arg Phe 675 680 685 Thr Ile Ala His Gln Asn Tyr Gln Pro Ile Val Lys Lys Ser Gly Ala 690 695 700 Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val Ser 705 710 715 720 Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala Ser 725 730 735 Pro Arg Pro Asn Asn Ala Ser Lys Glu Asp Ile Leu Lys Gln Gly Tyr 740 745 750 Gly Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu Glu 755 760 765 Leu Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly Met Ala Ala 770 775 780 Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr Ile 785 790 795 800 Asn Gly Ser His Tyr Glu Gln Asn Thr Ser Gly Ser Arg Thr His Tyr 805 810 815 Ala Val Lys Lys Thr Glu Glu Ile Glu Lys Gln Pro Ile Ile Leu Asp 820 825 830 Leu His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala Glu 835 840 845 Val Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala Gly 850 855 860 Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asp Ser Ile 865 870 875 880 Cys Ser Lys Gln Asn Asp Ile Cys Glu Gly Gly Gly Lys Asp Lys Thr 885 890 895 Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr

Tyr Ile Leu Ser Leu Asn 900 905 910 Tyr Lys Phe 915 59915PRTHaemophilus influenzae 86-028NP 59Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Leu 245 250 255 Asn Asp Ala Gly Gln Trp Thr Pro Asp Leu Ser Lys Asn Ala Trp Ser 260 265 270 Cys Asn Glu Asp Pro Pro Arg Leu Ala Glu Asn Ile Glu Asn Val Lys 275 280 285 Cys Thr His Tyr Ser Phe Asp Pro Arg Lys Asn Asp Arg Lys Glu Ile 290 295 300 Leu Lys Lys Leu Lys Ala Gly Thr Lys Pro Glu Asn Ile Asp Glu Leu 305 310 315 320 Gln Asn Gly Lys Asp Gly Ile Lys Glu Thr Asp Lys Ser Phe Glu Arg 325 330 335 Asn Lys Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln 340 345 350 Ser Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp His 355 360 365 Gln Asn Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys Ile Gly Ser 370 375 380 Arg Lys Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn Asn 385 390 395 400 Asn Asn Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly Lys 405 410 415 Thr Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Arg Val Ala Asp 420 425 430 Lys Leu Ile Thr Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn Ser 435 440 445 His Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly 450 455 460 Phe Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu 465 470 475 480 Leu Ser Leu Phe Tyr Asn Asp Asp Ser His Asp Gln Gly Asn Tyr Ser 485 490 495 Asn Leu Gly Arg Phe Arg Gly Asn Arg Asn Leu Leu Pro Gln Arg Ser 500 505 510 Val Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe 515 520 525 Asp Thr Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr Ser Val Asn 530 535 540 Phe Thr His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Asn Thr 545 550 555 560 Ala Asn Gln Ile Asn Glu Pro Ile Leu His Lys Ser Gly His Lys Lys 565 570 575 Ala Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe 580 585 590 Met Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln 595 600 605 Glu Met Phe Phe Ser Gln Val Ser Asn Ala Gly Val Asn Thr Ala Leu 610 615 620 Lys Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys 625 630 635 640 Lys Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Ile Lys Leu Val Gly 645 650 655 Tyr Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Val Trp 660 665 670 Trp Arg Asp Gly Val Val Pro Thr Trp Ala Asp Ser Thr Gly Phe Arg 675 680 685 Phe Thr Ile Ala His Gln Asn Tyr Gln Pro Ile Val Lys Lys Ser Gly 690 695 700 Ala Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val 705 710 715 720 Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala 725 730 735 Ser Ser Arg Pro Asn Asn Ala Ser Lys Glu Asp Ile Leu Lys Gln Gly 740 745 750 Tyr Gly Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu 755 760 765 Glu Ile Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly Met Ala 770 775 780 Ala Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr 785 790 795 800 Ile Asn Gly Ser His Phe Glu Leu Lys Thr Ser Gly Lys Arg Thr Tyr 805 810 815 Tyr Val Val Lys Lys Thr Glu Glu Ile Lys Lys Gln Pro Ile Ile Leu 820 825 830 Asp Leu His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala 835 840 845 Glu Val Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala 850 855 860 Gly Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asp Ser 865 870 875 880 Ile Cys Ser Lys Ser Gln Asp Cys Glu Asp Gly Gly Lys Asp Lys Thr 885 890 895 Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn 900 905 910 Tyr Lys Phe 915 60910PRTHaemophilus influenzae NT127 60Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ser Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Leu 245 250 255 Asn Pro Glu Gly Gln Trp Ala Pro Asp Leu Ser Lys Asn His Trp Ser 260 265 270 Cys Asn Lys Lys Asn Pro Glu Leu Val Asp Lys Arg Phe Ser Ser Phe 275 280 285 Arg Cys Asn Ile Tyr Ser Asn Ser Asp Arg Lys Glu Ile Leu Lys Glu 290 295 300 Leu Leu Glu Gln Asn Lys Asn Pro Lys Asp Ile Thr Lys Leu Gln Asn 305 310 315 320 Gly Lys Asp Gly Ile Lys Glu Thr Asp Glu Ser Phe Glu Arg Asn Lys 325 330 335 Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln Ser Arg 340 345 350 Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp His Gln Asn 355 360 365 Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys Ile Gly Ser Arg Lys 370 375 380 Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn Val Asn Asn Asn Ser 385 390 395 400 Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly Lys Thr Ile 405 410 415 Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Gln Val Ala Asp Lys Leu 420 425 430 Ile Thr Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn Ser His Thr 435 440 445 Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly Phe Asn 450 455 460 Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu Leu Ser 465 470 475 480 Leu Phe Tyr Lys Asp Asp Ser His Asp Arg Gly Asn Tyr Ser His Leu 485 490 495 Gly Arg Leu Ser Gly Ala Gln Gly Leu Leu Pro Gln Arg Ser Val Ile 500 505 510 Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe Asp Thr 515 520 525 Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr Ser Val Asn Phe Ile 530 535 540 His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Thr Thr Ala Glu 545 550 555 560 Pro Ile Leu His Lys Ser Gly His Lys Lys Ala Phe Asn His Ser Ala 565 570 575 Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe Met Pro Phe Phe Thr Tyr 580 585 590 Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu Met Phe Phe Ser Gln 595 600 605 Val Ser Asp Ala Gly Val Asn Thr Ala Leu Lys Pro Glu Gln Ser Asp 610 615 620 Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys Lys Gly Leu Phe Thr Gln 625 630 635 640 Asp Asp Val Leu Gly Ile Lys Leu Val Gly Tyr Arg Ser Phe Ile Lys 645 650 655 Asn Tyr Ile His Asn Val Tyr Gly Val Trp Trp Arg Asp Gly Lys Val 660 665 670 Pro Thr Trp Ala Asp Thr Asn Gly Phe Arg Phe Asn Ile Ala His Gln 675 680 685 Asn Tyr Gln Pro Ile Val Lys Lys Ser Gly Ala Glu Leu Glu Leu Asn 690 695 700 Tyr Asp Met Gly Arg Phe Phe Ala Asn Val Ser Tyr Ala Tyr Gln Arg 705 710 715 720 Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala Ser Pro Arg Pro Asn Asn 725 730 735 Ala Ser Lys Glu Asp Ile Leu Lys Gln Gly Tyr Gly Leu Ser Arg Val 740 745 750 Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu Glu Leu Gly Thr Arg Trp 755 760 765 Phe Asp Lys Lys Leu Thr Leu Gly Met Ala Ala Arg Tyr Tyr Gly Lys 770 775 780 Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr Ile Asn Gly Ser His Phe 785 790 795 800 Glu Lys Asn Thr Ala Arg Asn Arg Thr Tyr Tyr Ala Val Lys Lys Thr 805 810 815 Glu Glu Ile Lys Lys Gln Pro Ile Ile Leu Asp Leu His Val Ser Tyr 820 825 830 Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala Glu Val Gln Asn Leu Leu 835 840 845 Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala Gly Asn Asp Ala Ala Ser 850 855 860 Gln Arg Tyr Tyr Ser Ser Leu Asn Asp Ser Ile Cys Asn Lys Lys Ala 865 870 875 880 Asp Ser Cys Glu Gly Glu Gly Lys Asp Lys Ser Val Leu Tyr Asn Phe 885 890 895 Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn Tyr Lys Phe 900 905 910 61921PRTHaemophilus influenzae PittAA 61Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ser Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Leu 245 250 255 Asn Gln Ala Gly Gln Trp Ile Pro Asp Leu Asn Lys Asn His Trp Ser 260 265 270 Cys Asn His Pro Thr Glu Pro Lys Leu Ala Asp Pro Arg Lys Ile Gly 275 280 285 Asp Phe Thr Pro Glu Cys Lys Trp Tyr Asn Asn Asn Ser Glu Arg Lys 290 295 300 Glu Ile Leu Lys Gln Leu Ile Gln Glu Lys Lys Asp Pro Ser Glu Ile 305 310 315 320 Glu Lys Leu Gln Lys Gly Asp Asp Gly Ile Glu Lys Thr Asp Lys Ser 325 330 335 Phe Glu Asp Asn Lys Glu Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly 340 345 350 Ser Leu Gln Ser Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Ser 355 360 365 Asp Asp His His Thr Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys 370 375 380 Ile Gly Ser Arg Lys Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn 385 390 395 400 Phe Asn Asn Asn Ser Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn 405 410 415 Ile Gly Lys Thr Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Arg 420 425 430 Val Ala Asp Lys Leu Ile Thr Lys Asn Val Ala Asn Ile Val Asp Ile 435 440 445

Asn Asn Ser His Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr 450 455 460 Thr Leu Gly Phe Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe 465 470 475 480 Pro Glu Glu Leu Ser Leu Phe Tyr Val Asn Glu Ser His Asp Gln Gly 485 490 495 Leu Phe Ser Tyr Ser Gln Lys Gly Arg Tyr Ser Gly Thr Arg Gly Leu 500 505 510 Leu Pro Gln Arg Ser Val Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe 515 520 525 Lys Thr Val Tyr Phe Asp Thr Ala Leu Ser Lys Gly Ile Tyr His Leu 530 535 540 Asn Tyr Ser Val Asn Phe Thr His Tyr Ala Phe Asn Gly Glu Tyr Val 545 550 555 560 Gly Tyr Glu Asn Thr Glu Gly Lys Ile Asn Glu Pro Ile Leu His Lys 565 570 575 Ser Gly His Lys Lys Ala Phe Asn His Ser Ala Thr Leu Ser Ala Glu 580 585 590 Leu Ser Asp Tyr Phe Met Pro Phe Phe Thr Tyr Ser Arg Thr His Arg 595 600 605 Met Pro Asn Ile Gln Glu Met Phe Phe Ser Gln Val Ser Asp Ala Gly 610 615 620 Val Asn Thr Ala Leu Lys Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly 625 630 635 640 Phe Asn Thr Tyr Lys Lys Gly Leu Phe Thr Gln Asp Asp Val Leu Gly 645 650 655 Ile Lys Leu Val Gly Tyr Arg Ser Phe Ile Lys Asn Tyr Ile His Asn 660 665 670 Val Tyr Gly Asp Trp Ser Lys Asp Gly Val Thr Pro Ile Trp Ala Thr 675 680 685 Ala Asn Gly Phe Arg Leu Thr Ile Ala His Gln Asn Tyr Gln Pro Ile 690 695 700 Val Lys Lys Ser Gly Ala Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg 705 710 715 720 Phe Phe Ala Asn Phe Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr 725 730 735 Asn Tyr Ala Asp Ala Ser Ser Arg Pro Asn Asn Ala Ser Lys Gly Asp 740 745 750 Ile Leu Lys Gln Gly Tyr Gly Leu Ser Arg Ile Thr Met Leu Pro Lys 755 760 765 Asp Tyr Gly Arg Leu Glu Leu Gly Thr Arg Trp Phe Asp Gln Lys Leu 770 775 780 Thr Leu Gly Ile Ala Ala Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr 785 790 795 800 Thr Gln Glu Glu Tyr Ile Asn Gly Ser Arg Tyr Glu Lys Asn Thr Thr 805 810 815 Arg Asp Arg Ile Tyr Tyr Ala Ile Lys Lys Thr Glu Glu Ile Lys Lys 820 825 830 Gln Pro Ile Ile Leu Asp Leu His Val Ser Tyr Glu Pro Ile Lys Asp 835 840 845 Leu Ile Ile Lys Ala Glu Val Gln Asn Leu Leu Asp Lys Arg Tyr Val 850 855 860 Asp Pro Leu Asp Ala Gly Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser 865 870 875 880 Ser Leu Asn Asp Ser Leu Ala Cys Lys Ile Asn Glu Ser Thr Cys Asn 885 890 895 Asp Gly Ser Asp Lys Thr Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr 900 905 910 Tyr Ile Leu Ser Leu Asn Tyr Lys Phe 915 920 62915PRTHaemophilus influenzae PittHH 62Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ser Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Ile Leu 245 250 255 Asn Pro Glu Gly Gln Trp Ala Pro Asp Leu Asn Lys Pro His Trp Tyr 260 265 270 Cys Asn Lys Pro Asp Tyr Gln Ser Thr Ser Ser Asn Asn Glu Cys Arg 275 280 285 Arg Gly Tyr Arg Leu Gly Ser Ala Ala Gln Asp Arg Gln Asp Ile Leu 290 295 300 Lys Glu Leu Leu Thr Glu Asn Lys Lys Pro Glu Asn Ile Glu Lys Leu 305 310 315 320 Gln Lys Gly Asn Asp Gly Ile Lys Glu Thr Asp Lys Ser Phe Glu Arg 325 330 335 Asn Lys Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln 340 345 350 Ser Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp His 355 360 365 Gln Asn Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys Ile Gly Ser 370 375 380 Arg Lys Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn Asn 385 390 395 400 Asn Asn Tyr Leu Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly Lys 405 410 415 Thr Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Arg Val Ala Asp 420 425 430 Lys Leu Ile Thr Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn Ser 435 440 445 His Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly 450 455 460 Phe Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu 465 470 475 480 Leu Ser Leu Phe Tyr Asn Asp Asp Ser His Asp Gln Gly Thr Tyr Ser 485 490 495 Asn Leu Gly Arg Phe Lys Gly Asp Arg Asn Leu Leu Pro Gln Arg Ser 500 505 510 Val Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe 515 520 525 Asp Thr Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr Ser Val Asn 530 535 540 Phe Ile His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Asn Thr 545 550 555 560 Thr Ser Gln Ile Asn Glu Pro Ile Leu His Thr Ser Gly His Lys Lys 565 570 575 Ala Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe 580 585 590 Met Pro Phe Phe Thr Tyr Ser His Thr His Arg Met Pro Asn Ile Gln 595 600 605 Glu Met Phe Phe Ser Gln Val Ser Asp Ala Gly Val Asn Thr Ala Leu 610 615 620 Lys Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys 625 630 635 640 Lys Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Ile Lys Leu Val Gly 645 650 655 Tyr Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Val Trp 660 665 670 Trp Arg Asn Gly Val Val Pro Thr Trp Ala Asn Ser Thr Arg Phe Arg 675 680 685 Phe Thr Ile Ala His Gln Asn Tyr Gln Pro Ile Val Lys Lys Ser Gly 690 695 700 Ala Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val 705 710 715 720 Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala 725 730 735 Ser Pro Arg Pro Asn Asn Ala Ser Lys Glu Asp Ile Leu Lys Gln Gly 740 745 750 Tyr Gly Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu 755 760 765 Glu Leu Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly Met Ala 770 775 780 Ala Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr 785 790 795 800 Ile Asn Gly Ser Arg Tyr Glu Lys Tyr Thr Ala Gly Asp Arg Thr Tyr 805 810 815 Tyr Ala Val Lys Lys Thr Glu Glu Ile Lys Lys Gln Pro Ile Ile Leu 820 825 830 Asp Leu His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala 835 840 845 Glu Val Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala 850 855 860 Gly Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asn Ser 865 870 875 880 Ile Glu Cys Ala Gln Asp Ser Ser Ala Cys Gly Gly Ser Asp Lys Thr 885 890 895 Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn 900 905 910 Tyr Lys Phe 915 63915PRTHaemophilus influenzae PittII 63Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Ile Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Ser Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Thr Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Phe 245 250 255 Thr Asn Gly Gln Trp Thr Pro Asp Leu Asn Lys Asn His Trp Ser Cys 260 265 270 Asn Leu Pro Thr Pro Lys Leu Ala Asp Ser Thr Gln Leu Asn Gly Ile 275 280 285 Asn Glu Thr Ser Ala Cys Arg Leu Tyr Tyr Lys Asn Ser Lys Arg Lys 290 295 300 Glu Ile Leu Lys Glu Leu Glu Glu Lys Lys Asp Pro Ser Lys Ile Thr 305 310 315 320 Glu Leu Gln Lys Asp Ile Thr Glu Thr Asp Lys Ser Phe Glu Arg Asn 325 330 335 Lys Asp Gln Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln Ser 340 345 350 Arg Ser Arg Ser His Leu Leu Lys Phe Glu Tyr Ser Asp Asp Arg His 355 360 365 Thr Leu Gly Ala Gln Ile Arg Thr Leu Asp Asn Lys Ile Gly Ser Arg 370 375 380 Lys Ile Glu Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn Asn Asn 385 390 395 400 Ser Tyr Leu Asp Leu Asn Leu Met Thr Ala His Asn Ile Gly Lys Thr 405 410 415 Ile Tyr Pro Lys Gly Gly Phe Phe Ala Gly Trp Arg Val Ala Asp Lys 420 425 430 Leu Ile Thr Lys Asn Val Ala Asn Ile Ile Asp Ile Asn Asn Ser His 435 440 445 Thr Phe Leu Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly Phe 450 455 460 Asn Tyr Phe Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu Leu 465 470 475 480 Ser Leu Phe Tyr Asn Asp Asp Ser His Asn Gln Gly Asn Tyr Ser Tyr 485 490 495 Leu Gly Arg Phe Gln Gly Ser Lys Ser Gly Leu Pro Gln Arg Ser Val 500 505 510 Ile Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe Asp 515 520 525 Thr Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr Ser Val Asn Phe 530 535 540 Thr His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Asn Lys Pro 545 550 555 560 Thr Gln Ile Asn Glu Pro Ile Leu His Lys Ser Gly His Lys Lys Ala 565 570 575 Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe Met 580 585 590 Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu 595 600 605 Met Phe Phe Ser Gln Val Ser Asn Ala Gly Val Asn Thr Ala Leu Lys 610 615 620 Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys Lys 625 630 635 640 Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Ile Lys Leu Val Gly Tyr 645 650 655 Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Val Trp Trp 660 665 670 Arg Asp Gly Val Val Pro Thr Trp Ala Ser Ser Asn Gly Phe Arg Phe 675 680 685 Thr Ile Ala His Gln Asn Tyr Gln Pro Ile Val Lys Lys Ser Gly Ala 690 695 700 Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val Ser 705 710 715 720 Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala Ser 725 730 735 Pro Arg Pro Asn Asn Ala Ser Lys Asp Asp Ile Leu Lys Gln Gly Tyr 740 745 750 Gly Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu Glu 755 760 765 Leu Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly Met Ala Ala 770 775 780 Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr Ile 785 790 795 800 Asn Gly Ser Arg Tyr Glu Asn Tyr Thr Val Gly Asp Arg Thr Tyr Tyr 805 810 815 Ala Val Lys Lys Thr Glu Glu Ile Lys Lys Gln Pro Ile Ile Leu Asp 820 825 830 Leu His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala Glu 835 840 845 Val Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala Gly 850 855 860 Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asp Ser Ile 865 870 875 880 Cys Ser Lys Gly Asn Thr Thr Cys Glu Asp Gly Gly Lys Asp Lys Thr 885 890 895 Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn 900 905

910 Tyr Lys Phe 915 64934PRTHaemophilus influenzae R2846 64Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Leu Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ser Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Val Leu 245 250 255 Asn Ser Ala Gly Gln Trp Ala Pro Asp Leu Asn Lys Asn His Trp Ser 260 265 270 Cys Asn Asp Gln Thr Pro Lys Phe Asn Gly Ser Asn Glu Lys Thr Thr 275 280 285 Ser Thr Asn Pro Leu Thr Gln Glu Thr Glu Thr Ile Trp Thr Glu Ser 290 295 300 Asn Cys Ile Thr His Ile Gln Lys Asn Asp Glu Pro Glu Ile Thr Gln 305 310 315 320 Asn Ile Tyr Lys Asn Lys Asp Arg Glu Lys Ile Leu Glu Asp Leu Lys 325 330 335 Lys Asp Pro Asn Pro Glu Asn Ile Pro Glu Leu Gln Ala Glu Ile Lys 340 345 350 Lys Thr Asp Glu Ser Phe Glu Arg Asn Lys Asp Gln Tyr Ser Val Ala 355 360 365 Pro Ile Glu Pro Gly Ser Leu Gln Ser Arg Ser Arg Ser His Leu Leu 370 375 380 Lys Phe Glu Tyr Gly Asp Asp His Gln Asn Leu Gly Ala Gln Ile Arg 385 390 395 400 Thr Leu Asp Asn Lys Ile Gly Ser Arg Lys Ile Glu Asn Arg Asn Tyr 405 410 415 Gln Val Asn Tyr Asn Phe Asn Asn Asn Ser Tyr Leu Asp Leu Asn Leu 420 425 430 Met Ala Ala His Asn Ile Gly Lys Thr Ile Tyr Pro Lys Gly Gly Phe 435 440 445 Phe Ala Gly Trp Gln Val Ala Asp Lys Leu Ile Thr Lys Asn Val Ala 450 455 460 Asn Ile Val Asp Ile Asn Asn Ser His Thr Phe Leu Leu Pro Lys Glu 465 470 475 480 Ile Asp Leu Lys Thr Thr Leu Gly Phe Asn Tyr Phe Thr Asn Glu Tyr 485 490 495 Ser Lys Asn Arg Phe Pro Glu Glu Leu Ser Leu Phe Tyr Asn Asp Asp 500 505 510 Ser His Asp Arg Gly Asn Tyr Ser Asn Leu Gly Arg Leu Lys Gly Ala 515 520 525 Gln Gly Leu Leu Pro Gln Arg Ser Val Ile Leu Gln Pro Ser Gly Lys 530 535 540 Gln Lys Phe Lys Thr Val Tyr Phe Asp Thr Ala Leu Ser Lys Gly Ile 545 550 555 560 Tyr His Leu Asn Tyr Ser Val Asn Phe Thr His Tyr Ala Phe Asn Gly 565 570 575 Glu Tyr Val Gly Tyr Glu Asn Thr Ala Glu Pro Ile Leu His Lys Ser 580 585 590 Gly His Lys Lys Ala Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu 595 600 605 Ser Asp Tyr Phe Met Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met 610 615 620 Pro Asn Ile Gln Glu Met Phe Phe Ser Gln Val Ser Asp Ala Gly Val 625 630 635 640 Asn Thr Ala Leu Lys Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe 645 650 655 Asn Thr Tyr Lys Lys Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Ile 660 665 670 Lys Leu Val Gly Tyr Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val 675 680 685 Tyr Gly Val Trp Trp Arg Asn Gly Lys Val Pro Asp Trp Ala Ala Thr 690 695 700 Asn Gly Phe Arg Phe Asn Ile Ala His Gln Asn Tyr Gln Pro Ile Val 705 710 715 720 Lys Lys Ser Gly Ala Glu Leu Glu Leu Asn Tyr Asp Met Gly Arg Phe 725 730 735 Phe Ala Asn Val Ser Tyr Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn 740 745 750 Tyr Ala Asp Ala Ser Pro Arg Pro Asn Asn Ala Ser Lys Glu Asp Ile 755 760 765 Leu Lys Gln Gly Tyr Gly Leu Ser Arg Val Ser Met Leu Pro Lys Asp 770 775 780 Tyr Gly Arg Leu Glu Leu Gly Thr Arg Trp Phe Asp Lys Lys Leu Thr 785 790 795 800 Leu Gly Met Ala Ala Arg Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile 805 810 815 Glu Glu Glu Tyr Ile Asn Gly Ser His Phe Glu Lys Asn Thr Ser Gly 820 825 830 Ser Arg Thr Tyr Tyr Ala Val Lys Lys Thr Glu Glu Ile Lys Lys Gln 835 840 845 Pro Ile Ile Leu Asp Leu His Val Ser Tyr Glu Pro Ile Lys Asp Leu 850 855 860 Ile Ile Lys Ala Glu Val Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp 865 870 875 880 Pro Leu Asp Ala Gly Asn Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser 885 890 895 Leu Asn Asp Ser Ile Cys Lys Gly Asn Thr Cys Glu Asp Gly Gly Lys 900 905 910 Asp Lys Thr Val Leu Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu 915 920 925 Ser Leu Asn Tyr Lys Phe 930 65929PRTHaemophilus influenzae R2866 65Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly Gln Ile Asp 1 5 10 15 Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe Thr Glu Ala 20 25 30 Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr Gln Thr Ile 35 40 45 Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln Gln Asp Lys 50 55 60 Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn Gly Leu Gly 65 70 75 80 Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe Tyr Ser Thr 85 90 95 Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe Gly Ala Ala 100 105 110 Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys Ser Asn Phe 115 120 125 Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala Asn Phe Arg 130 135 140 Thr Leu Ser Val Asn Asp Val Ile Thr Asp Asp Lys Pro Phe Gly Ile 145 150 155 160 Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser Asn Phe Met 165 170 175 Thr Thr Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly Tyr Val Gly 180 185 190 Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp Tyr Arg Ile 195 200 205 Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile Leu Ala Lys 210 215 220 Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Ile Leu Asn Pro Ala 225 230 235 240 Gly Gln Trp Ala Pro Asp Leu Ser Lys Asn His Trp Ser Cys Asn Ala 245 250 255 Pro Asp Pro Lys Phe Asn Gly Ser Thr Lys Lys Thr Glu Ser Ser Asn 260 265 270 Asp Leu Thr Gly Glu Arg Glu Thr Ile Trp Thr Asp Lys Asp Cys Val 275 280 285 Phe His Arg Glu Lys Tyr Asn Ile Tyr Asp Val Pro Thr Lys Lys Glu 290 295 300 Glu Thr Lys Asp Lys Ile Tyr Glu Asp Pro Val Arg Lys Lys Ile Leu 305 310 315 320 Glu Asp Ile Asn Lys Asn Thr Pro Leu Lys Asp Ile Pro Glu Leu Gln 325 330 335 Lys Gly Ile Glu Lys Thr Asp Lys Ser Phe Glu Asp Asn Lys Glu Gln 340 345 350 Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln Ser Arg Ser Arg 355 360 365 Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp His His Thr Leu Gly 370 375 380 Ala Gln Ile Arg Thr Leu Asp Asn Lys Ile Gly Ser Arg Lys Ile Glu 385 390 395 400 Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn Asn Asn Ser Tyr Leu 405 410 415 Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly Lys Thr Ile Tyr Pro 420 425 430 Lys Gly Gly Phe Phe Ala Gly Trp Arg Val Arg Asp Lys Leu Ile Thr 435 440 445 Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn Ser His Thr Phe Leu 450 455 460 Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly Phe Asn Tyr Phe 465 470 475 480 Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu Leu Ser Leu Phe 485 490 495 Tyr Lys Glu Ala Ser Gly Asp Pro Gly Arg Tyr Thr Tyr Thr Gln Gly 500 505 510 Thr Gly Gly Thr Gln Asn Leu Leu Pro Gln Arg Ser Val Ile Leu Gln 515 520 525 Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe Asp Thr Ala Leu 530 535 540 Ser Lys Gly Ile Tyr His Leu Asn Tyr Asn Val Asn Phe Thr His Tyr 545 550 555 560 Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Asn Lys Pro Thr Gln Ile 565 570 575 Asn Glu Pro Ile Leu His Lys Ser Gly His Lys Lys Ala Phe Asn His 580 585 590 Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe Met Pro Phe Phe 595 600 605 Thr Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu Met Phe Phe 610 615 620 Ser Gln Val Ser Asn Ala Gly Val Asn Thr Ala Leu Lys Pro Glu Gln 625 630 635 640 Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys Lys Gly Leu Phe 645 650 655 Thr Gln Asp Asp Val Leu Gly Val Lys Leu Val Gly Tyr Arg Ser Phe 660 665 670 Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Glu Trp Trp Lys Asn Asp 675 680 685 Val Pro Ile Trp Ala Asp Ser Asn Gly Phe Arg Phe Thr Ile Ala His 690 695 700 Gln Asn Tyr Lys Pro Ile Val Lys Lys Ser Gly Val Glu Leu Glu Ile 705 710 715 720 Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val Ser Tyr Ala Tyr Gln 725 730 735 Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala Ser Pro Arg Ala Lys 740 745 750 Asn Thr Ser Asn Lys Asp Ile Leu Lys Gln Gly Tyr Gly Leu Ser Arg 755 760 765 Ile Thr Met Leu Pro Arg Asp Tyr Gly Arg Leu Glu Leu Gly Thr Arg 770 775 780 Trp Phe Asp Gln Lys Leu Thr Leu Gly Met Ala Ala Arg Tyr Tyr Gly 785 790 795 800 Lys Ser Lys Arg Ala Ser Ile Lys Asp Glu Cys Val Lys Gly Val Pro 805 810 815 Cys Lys Ile Gln Gly Thr Gly Glu Lys Ala Glu Val Val His Asn Ala 820 825 830 Ile Lys Lys Thr Glu Asp Ile Lys Lys Gln Pro Ile Ile Leu Asp Leu 835 840 845 His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala Glu Val 850 855 860 Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala Gly Asn 865 870 875 880 Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asp Ser Ile Cys 885 890 895 Ser Lys Asp Gly Thr Cys Glu Asp Gly Gly Lys Asp Lys Pro Val Leu 900 905 910 Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn Tyr Lys 915 920 925 Phe 66913PRTHaemophilus influenzae Rd KW20 66Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Ile Leu 245 250 255 Asn Pro Glu Gly Gln Trp Thr Pro Asp Leu Ser Lys Lys His Trp Ser 260 265 270 Cys Asn Lys Pro Asp Tyr Gln Lys Asn Gly Asp Cys Ser Tyr Tyr Arg 275 280 285 Ile Gly Ser Ala Ala Lys Thr Arg Arg Glu Ile Leu Gln Glu Leu Leu 290 295 300 Thr Asn Gly Lys Lys Pro Lys Asp Ile Glu Lys Leu Gln Lys Gly Asn 305 310 315 320 Asp Gly Ile Glu Glu Thr Asp Lys Ser Phe Glu Arg Asn Lys Asp Gln 325 330 335 Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln Ser Arg Ser Arg 340 345 350 Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp His Gln Asn Leu Gly 355 360 365 Ala Gln Leu Arg Thr Leu Asp Asn Lys Ile Gly Ser Arg Lys Ile Glu 370 375 380 Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn Asn Asn Ser Tyr Leu 385 390 395 400 Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly Lys Thr Ile Tyr Pro 405 410 415 Lys Gly Gly Phe Phe Ala Gly Trp Gln

Val Ala Asp Lys Leu Ile Thr 420 425 430 Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn Ser His Thr Phe Leu 435 440 445 Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly Phe Asn Tyr Phe 450 455 460 Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu Leu Ser Leu Phe 465 470 475 480 Tyr Asn Asp Ala Ser His Asp Gln Gly Leu Tyr Ser His Ser Lys Arg 485 490 495 Gly Arg Tyr Ser Gly Thr Lys Ser Leu Leu Pro Gln Arg Ser Val Ile 500 505 510 Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe Asp Thr 515 520 525 Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr Ser Val Asn Phe Thr 530 535 540 His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Asn Thr Ala Gly 545 550 555 560 Gln Gln Ile Asn Glu Pro Ile Leu His Lys Ser Gly His Lys Lys Ala 565 570 575 Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe Met 580 585 590 Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu 595 600 605 Met Phe Phe Ser Gln Val Ser Asn Ala Gly Val Asn Thr Ala Leu Lys 610 615 620 Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys Lys 625 630 635 640 Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Val Lys Leu Val Gly Tyr 645 650 655 Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Val Trp Trp 660 665 670 Arg Asp Gly Met Pro Thr Trp Ala Glu Ser Asn Gly Phe Lys Tyr Thr 675 680 685 Ile Ala His Gln Asn Tyr Lys Pro Ile Val Lys Lys Ser Gly Val Glu 690 695 700 Leu Glu Ile Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val Ser Tyr 705 710 715 720 Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala Ser Pro 725 730 735 Arg Pro Asn Asn Ala Ser Gln Glu Asp Ile Leu Lys Gln Gly Tyr Gly 740 745 750 Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu Glu Leu 755 760 765 Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly Leu Ala Ala Arg 770 775 780 Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr Ile Asn 785 790 795 800 Gly Ser Arg Phe Lys Lys Asn Thr Leu Arg Arg Glu Asn Tyr Tyr Ala 805 810 815 Val Lys Lys Thr Glu Asp Ile Lys Lys Gln Pro Ile Ile Leu Asp Leu 820 825 830 His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala Glu Val 835 840 845 Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala Gly Asn 850 855 860 Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asn Ser Ile Glu 865 870 875 880 Cys Ala Gln Asp Ser Ser Ala Cys Gly Gly Ser Asp Lys Thr Val Leu 885 890 895 Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn Tyr Lys 900 905 910 Phe 67913PRTHaemophilus influenzae RdAW 67Met Lys Lys Ala Ile Lys Leu Asn Leu Ile Thr Leu Gly Leu Ile Asn 1 5 10 15 Thr Ile Gly Met Thr Ile Thr Gln Ala Gln Ala Glu Glu Thr Leu Gly 20 25 30 Gln Ile Asp Val Val Glu Lys Val Ile Ser Asn Asp Lys Lys Pro Phe 35 40 45 Thr Glu Ala Lys Ala Lys Ser Thr Arg Glu Asn Val Phe Lys Glu Thr 50 55 60 Gln Thr Ile Asp Gln Val Ile Arg Ser Ile Pro Gly Ala Phe Thr Gln 65 70 75 80 Gln Asp Lys Gly Ser Gly Val Val Ser Val Asn Ile Arg Gly Glu Asn 85 90 95 Gly Leu Gly Arg Val Asn Thr Met Val Asp Gly Val Thr Gln Thr Phe 100 105 110 Tyr Ser Thr Ala Leu Asp Ser Gly Gln Ser Gly Gly Ser Ser Gln Phe 115 120 125 Gly Ala Ala Ile Asp Pro Asn Phe Ile Ala Gly Val Asp Val Asn Lys 130 135 140 Ser Asn Phe Ser Gly Ala Ser Gly Ile Asn Ala Leu Ala Gly Ser Ala 145 150 155 160 Asn Phe Arg Thr Leu Gly Val Asn Asp Val Ile Thr Asp Asp Lys Pro 165 170 175 Phe Gly Ile Ile Leu Lys Gly Met Thr Gly Ser Asn Ala Thr Lys Ser 180 185 190 Asn Phe Met Thr Met Ala Ala Gly Arg Lys Trp Leu Asp Asn Gly Gly 195 200 205 Tyr Val Gly Val Val Tyr Gly Tyr Ser Gln Arg Glu Val Ser Gln Asp 210 215 220 Tyr Arg Ile Gly Gly Gly Glu Arg Leu Ala Ser Leu Gly Gln Asp Ile 225 230 235 240 Leu Ala Lys Glu Lys Glu Ala Tyr Phe Arg Asn Ala Gly Tyr Ile Leu 245 250 255 Asn Pro Glu Gly Gln Trp Thr Pro Asp Leu Ser Lys Lys His Trp Ser 260 265 270 Cys Asn Lys Pro Asp Tyr Gln Lys Asn Gly Asp Cys Ser Tyr Tyr Arg 275 280 285 Ile Gly Ser Ala Ala Lys Thr Arg Arg Glu Ile Leu Gln Glu Leu Leu 290 295 300 Thr Asn Gly Lys Lys Pro Lys Asp Ile Glu Lys Leu Gln Lys Gly Asn 305 310 315 320 Asp Gly Ile Glu Glu Thr Asp Lys Ser Phe Glu Arg Asn Lys Asp Gln 325 330 335 Tyr Ser Val Ala Pro Ile Glu Pro Gly Ser Leu Gln Ser Arg Ser Arg 340 345 350 Ser His Leu Leu Lys Phe Glu Tyr Gly Asp Asp His Gln Asn Leu Gly 355 360 365 Ala Gln Leu Arg Thr Leu Asp Asn Lys Ile Gly Ser Arg Lys Ile Glu 370 375 380 Asn Arg Asn Tyr Gln Val Asn Tyr Asn Phe Asn Asn Asn Ser Tyr Leu 385 390 395 400 Asp Leu Asn Leu Met Ala Ala His Asn Ile Gly Lys Thr Ile Tyr Pro 405 410 415 Lys Gly Gly Phe Phe Ala Gly Trp Gln Val Ala Asp Lys Leu Ile Thr 420 425 430 Lys Asn Val Ala Asn Ile Val Asp Ile Asn Asn Ser His Thr Phe Leu 435 440 445 Leu Pro Lys Glu Ile Asp Leu Lys Thr Thr Leu Gly Phe Asn Tyr Phe 450 455 460 Thr Asn Glu Tyr Ser Lys Asn Arg Phe Pro Glu Glu Leu Ser Leu Phe 465 470 475 480 Tyr Asn Asp Ala Ser His Asp Gln Gly Leu Tyr Ser His Ser Lys Arg 485 490 495 Gly Arg Tyr Ser Gly Thr Lys Ser Leu Leu Pro Gln Arg Ser Val Ile 500 505 510 Leu Gln Pro Ser Gly Lys Gln Lys Phe Lys Thr Val Tyr Phe Asp Thr 515 520 525 Ala Leu Ser Lys Gly Ile Tyr His Leu Asn Tyr Ser Val Asn Phe Thr 530 535 540 His Tyr Ala Phe Asn Gly Glu Tyr Val Gly Tyr Glu Asn Thr Ala Gly 545 550 555 560 Gln Gln Ile Asn Glu Pro Ile Leu His Lys Ser Gly His Lys Lys Ala 565 570 575 Phe Asn His Ser Ala Thr Leu Ser Ala Glu Leu Ser Asp Tyr Phe Met 580 585 590 Pro Phe Phe Thr Tyr Ser Arg Thr His Arg Met Pro Asn Ile Gln Glu 595 600 605 Met Phe Phe Ser Gln Val Ser Asn Ala Gly Val Asn Thr Ala Leu Lys 610 615 620 Pro Glu Gln Ser Asp Thr Tyr Gln Leu Gly Phe Asn Thr Tyr Lys Lys 625 630 635 640 Gly Leu Phe Thr Gln Asp Asp Val Leu Gly Val Lys Leu Val Gly Tyr 645 650 655 Arg Ser Phe Ile Lys Asn Tyr Ile His Asn Val Tyr Gly Val Trp Trp 660 665 670 Arg Asp Gly Met Pro Thr Trp Ala Glu Ser Asn Gly Phe Lys Tyr Thr 675 680 685 Ile Ala His Gln Asn Tyr Lys Pro Ile Val Lys Lys Ser Gly Val Glu 690 695 700 Leu Glu Ile Asn Tyr Asp Met Gly Arg Phe Phe Ala Asn Val Ser Tyr 705 710 715 720 Ala Tyr Gln Arg Thr Asn Gln Pro Thr Asn Tyr Ala Asp Ala Ser Pro 725 730 735 Arg Pro Asn Asn Ala Ser Gln Glu Asp Ile Leu Lys Gln Gly Tyr Gly 740 745 750 Leu Ser Arg Val Ser Met Leu Pro Lys Asp Tyr Gly Arg Leu Glu Leu 755 760 765 Gly Thr Arg Trp Phe Asp Gln Lys Leu Thr Leu Gly Leu Ala Ala Arg 770 775 780 Tyr Tyr Gly Lys Ser Lys Arg Ala Thr Ile Glu Glu Glu Tyr Ile Asn 785 790 795 800 Gly Ser Arg Phe Lys Lys Asn Thr Leu Arg Arg Glu Asn Tyr Tyr Ala 805 810 815 Val Lys Lys Thr Glu Asp Ile Lys Lys Gln Pro Ile Ile Leu Asp Leu 820 825 830 His Val Ser Tyr Glu Pro Ile Lys Asp Leu Ile Ile Lys Ala Glu Val 835 840 845 Gln Asn Leu Leu Asp Lys Arg Tyr Val Asp Pro Leu Asp Ala Gly Asn 850 855 860 Asp Ala Ala Ser Gln Arg Tyr Tyr Ser Ser Leu Asn Asn Ser Ile Glu 865 870 875 880 Cys Ala Gln Asp Ser Ser Ala Cys Gly Gly Ser Asp Lys Thr Val Leu 885 890 895 Tyr Asn Phe Ala Arg Gly Arg Thr Tyr Ile Leu Ser Leu Asn Tyr Lys 900 905 910 Phe 68915PRTHaemophilus somnus 129PT 68Met Ser Asn Lys Phe Lys Leu Thr Ala Val Ala Phe Ser Val Leu Ser 1 5 10 15 Thr Cys Val Tyr Ala Glu Glu Met Leu Asp Gln Ile Asn Val Glu Tyr 20 25 30 Lys Leu Pro Pro Lys Gln Glu Val Phe Lys Lys Ala Gly Ala Thr Ser 35 40 45 Val Arg Glu Asn Ile Ser Thr Ser Thr Gln Ser Ile Asp Asp Ile Ile 50 55 60 Arg Thr Val Pro Gly Ala Phe Thr Asn Leu Asp Lys Ser Ser Gly Ile 65 70 75 80 Val Ser Val Asn Val Arg Gly Gln Thr Gly Phe Gly Arg Val Asn Thr 85 90 95 Met Val Asp Gly Ile Ser Gln Thr Phe Phe Ala Thr Ser Gly Asp Asn 100 105 110 Ser Glu Lys Ala Gly Gly Thr Ser Gln Phe Gly Ala Thr Ile Asp Pro 115 120 125 Ala Phe Ile Thr Ser Val Asp Ile Gln Arg Gly Ser Phe Asp Gly Lys 130 135 140 Ala Gly Ala Asn Ala Leu Val Gly Ser Ala Asn Phe Arg Thr Ile Gly 145 150 155 160 Val Asp Asp Val Ile Ser Glu Gly Arg Asn Val Gly Ala Leu Leu Asn 165 170 175 Ala Gly Gly Gly Thr Asn Ala Thr Gly Pro Asn Tyr Met Gly Ala Val 180 185 190 Ala Phe Arg Tyr Pro Phe Ser Asp Thr Arg Thr Leu Gly Phe Met Tyr 195 200 205 Ala Tyr Ser Trp Arg Lys Ser Ser Gln Asp Phe Lys Val Gly Gly Gly 210 215 220 Arg Ser Ile Lys Asp Val Asp Ala Glu Glu Val Gly Arg Ile Val Lys 225 230 235 240 Glu Ala Cys Lys Gly Glu Phe Leu Cys Lys Glu Asp Glu Lys Arg Lys 245 250 255 Leu Tyr Gly Leu Gln Pro Tyr Asp Pro Ser Lys Leu Leu His Tyr Pro 260 265 270 Gln Ser His Leu Ala Lys Ile Glu Tyr Asn Asp Lys Tyr Asn Lys Ala 275 280 285 Ile Leu Ser Tyr Arg Ser Leu Ser Thr Ala Leu Ser Gly Arg Asn Leu 290 295 300 Glu Asn Lys Asn Tyr Gln Leu Asn Tyr Gln Leu Lys Pro Lys Glu Asn 305 310 315 320 Leu Gln Phe Glu Leu Ile Leu Ala Asp Asn Thr Ser Thr Gln Thr Tyr 325 330 335 His Lys Gly Ser Val Phe Asn Gly Lys Met Leu Lys Asp Val Leu Glu 340 345 350 Ser Lys Asn Ser Ala Lys Thr Thr Asp Ile Asn Met His Phe Ser Ile 355 360 365 Pro Phe Thr Glu Lys Phe Ser Tyr Glu Ala Thr Leu Gly Ala Asn Ile 370 375 380 Leu Lys Asn Lys Tyr Ser Lys Asn Arg His Pro Arg Glu Leu Gly Tyr 385 390 395 400 Tyr Phe Asp Gly Gly Glu Pro Asn Ser Arg Asp Phe Cys Asp Tyr Val 405 410 415 Gly Lys Tyr Lys Asn Ala Cys Ile Tyr His Gly Ile Arg Ser Asn Thr 420 425 430 Phe Gln Pro Asp Gly Glu Gln Arg Phe Lys Thr Leu Tyr Phe Asp Gln 435 440 445 Thr Phe Lys Tyr Asp Ile Tyr Thr Leu Lys Val Asn Ala Asn Arg Gln 450 455 460 Ile Tyr Lys Tyr Ser Gly Ala Thr Phe Pro Arg Asp Pro Arg Asp Arg 465 470 475 480 Val Thr Arg Tyr Phe Pro Ala Asp Pro Glu Gln Lys Thr Lys Asp Gly 485 490 495 Tyr Thr Val Tyr Val Asn Gly Asn Ser Ser Ala Ile Val His Lys Tyr 500 505 510 Gly Tyr Asp Gly Ala Ile Lys Phe Gly Cys Tyr His Tyr Glu Asn Asp 515 520 525 Glu Glu Gln Trp Val Asp Val Tyr Asn Cys Pro Thr Phe Tyr Ser Val 530 535 540 Gln Lys Gly Asn Ser Arg Ala His Asp Asn Tyr Ser Met Ser Phe Ser 545 550 555 560 Ala Asp Phe His Pro Leu Phe Ser Pro Phe Val Ser Tyr Ala Lys Thr 565 570 575 His Arg Val Pro Asn Ile Lys Glu Met Tyr Phe Ser Glu Phe Gly Thr 580 585 590 Leu Ala Ile Arg Pro Asp Leu Lys Ser Glu Lys Ala Lys Thr Ile Gln 595 600 605 Phe Gly Ile Asn Gly Tyr Lys Asn Gly Ile Phe Ser Asp Phe Asp Ala 610 615 620 Leu Gly Phe Lys Val Leu Ala Tyr Gln Thr Lys Ile Arg Asp Tyr Ile 625 630 635 640 Ile Asn Ile Lys Gln Glu Cys Ser Ser Asn Ser Trp Arg Gly Ala Asp 645 650 655 Lys Ala Pro Thr Ile Tyr Asp Cys Val Gly His Leu Thr His Ile Asn 660 665 670 Tyr Glu Gly Gly Lys Thr Ile Leu Lys Thr Glu Met Val Asp Ser Gly 675 680 685 Ser Gly Phe Lys Val Pro Leu Asn Gln Leu Ile Pro Ala Asp Ser Ser 690 695 700 Pro Ile Val Lys Ile Gln Gly Leu Glu Leu Glu Leu Asn Tyr Asp Ile 705 710 715 720 Gly Trp Phe Tyr Ala Asn Leu Ser Tyr Ala Arg Gln Lys Thr Asn Gln 725 730 735 Pro Ser Ser Tyr Ser Asp Ser Ser Asn Ser Val Gly Asp Asp Ser Glu 740 745 750 Thr Gly Gln Tyr Leu Gln Gly Phe Gly Leu Ser Lys Ile Ser Ser Leu 755 760 765 Pro Lys Asp Tyr Gly Ser Leu Asp Leu Gly Thr Arg Leu Phe Asp Gly 770 775 780 Lys Leu Thr Leu Gly Gly Thr Ala Lys Tyr Tyr Gly Lys Ser Lys Arg 785 790 795 800 Ala Arg Leu Asp Lys Ala Asp Gly Asp Val Ile Leu Pro Gly Thr Phe 805 810 815 Thr Ser Arg Lys Thr Gly Glu Thr Phe Leu Thr Tyr Val Arg Ile Ser 820 825 830 Gly Thr Glu Glu Ile Lys Ala Gln Pro Ile Ile Phe Asp Phe Tyr Ala 835 840 845 Ile Tyr Gln Pro Thr Glu Asn Leu Thr Ile Lys Gly Glu Ile Gln Asn 850 855 860 Ala Phe Asp Lys Lys Tyr Ile Asn Pro Leu Asp Ala Asn Asn Asp Ser 865 870 875 880 Ala Asn Gln Met Thr Tyr Ala Met Gly Val Gly Asp Gly Tyr Gln Lys 885 890

895 Ala Leu Asn Asn Tyr Ser Lys Gly Arg Thr Phe Val Leu Asn Val Asn 900 905 910 Tyr Lys Phe 915 69917PRTHaemophilus somnus 2336 69Met Met Thr Asn Lys Phe Lys Leu Thr Ala Val Ala Phe Ser Val Leu 1 5 10 15 Ser Thr Cys Val Tyr Ala Glu Glu Met Leu Asp Gln Ile Asn Val Glu 20 25 30 Tyr Lys Leu Pro Pro Lys Gln Glu Val Phe Lys Lys Ala Gly Ala Thr 35 40 45 Ser Val Arg Glu Asn Ile Ser Thr Ser Thr Gln Ser Ile Asp Asp Ile 50 55 60 Ile Arg Thr Val Pro Gly Ala Phe Thr Asn Leu Asp Lys Ser Ser Gly 65 70 75 80 Thr Val Ser Val Asn Val Arg Gly Gln Thr Gly Phe Gly Arg Val Asn 85 90 95 Thr Met Val Asp Gly Ile Ser Gln Thr Phe Phe Ala Thr Ser Gly Asp 100 105 110 Asn Ser Glu Lys Ala Gly Gly Thr Ser Gln Phe Gly Ala Thr Ile Asp 115 120 125 Pro Ala Phe Ile Thr Ser Val Asp Ile Gln Arg Gly Ser Phe Asp Gly 130 135 140 Lys Ala Gly Ala Asn Ala Leu Val Gly Ser Ala Asn Phe Arg Thr Ile 145 150 155 160 Gly Val Asp Asp Val Ile Ser Glu Gly Arg Asn Val Gly Ala Leu Leu 165 170 175 Asn Val Gly Gly Gly Thr Asn Ala Thr Gly Pro Asn Tyr Met Gly Ala 180 185 190 Val Ala Phe Arg Tyr Pro Phe Ser Asp Thr Arg Thr Leu Gly Phe Met 195 200 205 Tyr Ala Tyr Ser Trp Arg Lys Ser Ser Gln Asp Phe Lys Val Gly Gly 210 215 220 Gly Arg Ser Ile Lys Asp Val Asp Ala Glu Glu Val Gly Arg Ile Val 225 230 235 240 Glu Glu Ala Cys Lys Gly Glu Ala Phe Cys Asp Arg Asn Glu Lys Arg 245 250 255 Lys Leu Tyr Gly Leu Gln Pro Tyr Asp Pro Ser Lys Leu Leu His Tyr 260 265 270 Pro Gln Ser His Leu Ala Lys Ile Glu Tyr Asn Asp Lys Tyr Asn Lys 275 280 285 Ala Ile Leu Ser Tyr Arg Ser Leu Ser Thr Ala Leu Ser Gly Arg Asn 290 295 300 Leu Glu Asn Lys Asn Tyr Gln Leu Asn Tyr Gln Leu Lys Pro Lys Glu 305 310 315 320 Asn Leu Gln Phe Glu Leu Ile Leu Ala Asp Asn Thr Ser Thr Gln Thr 325 330 335 Tyr His Lys Gly Ser Val Phe Asn Gly Lys Met Leu Lys Asp Val Leu 340 345 350 Glu Ser Lys Asn Ser Ala Lys Thr Thr Asp Ile Asn Met His Phe Ser 355 360 365 Ile Pro Phe Thr Glu Lys Phe Ser Tyr Glu Ala Thr Leu Gly Ala Asn 370 375 380 Ile Leu Lys Asn Lys Tyr Ser Lys Asn Arg His Pro Arg Glu Leu Gly 385 390 395 400 Tyr Tyr Phe Asp Gly Gly Glu Pro Asn Ser Leu Asp Ala Cys Asp Glu 405 410 415 Val Gly Lys Tyr Lys Asn Ala Cys Lys Tyr His Gly Ile Arg Ser Asn 420 425 430 Thr Phe Gln Pro Asp Gly Glu Gln Arg Phe Lys Thr Leu Tyr Phe Asp 435 440 445 Gln Thr Phe Lys Tyr Asp Ile Tyr Thr Leu Lys Val Asn Ala Asn Arg 450 455 460 Gln Ile Tyr Lys Tyr Glu Gly Arg Ile Phe Pro Leu Asp Pro Lys Asp 465 470 475 480 Arg Phe Ser Ser Arg Thr Trp His Asp Ser His Lys Lys Met Lys Asn 485 490 495 Gly Lys Pro Ile Trp Val Asn Ser Asn Ser Ser Glu Ile Val Ala Lys 500 505 510 Tyr Gly Val Glu Glu Ala Glu Lys Leu Gly Cys Tyr Thr Gly Pro Asn 515 520 525 Asp Asp Asp Thr Thr Trp Asp Val Tyr Asn Cys Pro Ser Phe Tyr Ser 530 535 540 Val His Lys Gly Asp Ser Arg Ser His Asp Asn Tyr Ser Met Ser Phe 545 550 555 560 Ser Ala Asp Phe His Pro Leu Phe Ser Pro Phe Val Ser Tyr Ala Lys 565 570 575 Thr His Arg Val Pro Asn Ile Lys Glu Met Tyr Phe Ser Glu Phe Gly 580 585 590 Thr Leu Ala Ile Arg Pro Asp Leu Lys Ser Glu Lys Ala Lys Thr Ile 595 600 605 Gln Phe Gly Ile Asn Gly Tyr Lys Asn Gly Ile Phe Ser Asp Phe Asp 610 615 620 Ala Leu Gly Phe Lys Ile Leu Ala Tyr Gln Thr Lys Ile Arg Asp Tyr 625 630 635 640 Ile Ile Asn Ile Lys Gln Glu Cys Ser Ser Asn Ala Trp Arg Gly Ser 645 650 655 Asn Lys Ala Pro Thr Ile Tyr Asp Cys Val Glu His Leu Thr His Ile 660 665 670 Asn Tyr Glu Gly Gly Lys Thr Ile Leu Lys Thr Asp Leu Val Asp Thr 675 680 685 Gly Asp Gly Lys Thr Lys Val Pro Ser Asn Gln Leu Ile Pro Ala Asp 690 695 700 Ser Ser Pro Ile Val Lys Ile Gln Gly Leu Glu Leu Glu Leu Asn Tyr 705 710 715 720 Asp Ile Gly Trp Phe Tyr Ala Asn Leu Ser Tyr Ala Arg Gln Lys Thr 725 730 735 Asn Gln Pro Ser Ser Phe Ser Asp Ser Ser Tyr Asn Val Ser Asp Asp 740 745 750 Ser Glu Thr Gly Gln Tyr Leu Gln Gly Phe Gly Leu Ser Lys Ile Ser 755 760 765 Ser Leu Pro Lys Asp Tyr Gly Ser Leu Asp Leu Gly Thr Arg Leu Phe 770 775 780 Asp Gly Lys Leu Thr Leu Gly Gly Thr Ala Lys Tyr Tyr Gly Lys Ser 785 790 795 800 Lys Arg Ala Arg Leu Asp Lys Ala Asp Gly Asp Val Ile Leu Pro Gly 805 810 815 Thr Phe Thr Ser Arg Lys Thr Gly Glu Thr Phe Leu Thr Tyr Val Arg 820 825 830 Ile Ser Gly Thr Glu Glu Ile Lys Ala Gln Pro Ile Ile Phe Asp Phe 835 840 845 Tyr Ala Ile Tyr Gln Pro Thr Glu Asn Leu Thr Ile Lys Gly Glu Ile 850 855 860 Gln Asn Ala Phe Asp Lys Lys Tyr Ile Asn Pro Leu Asp Ala Asn Asn 865 870 875 880 Asp Ser Ala Asn Gln Met Thr Tyr Ala Met Gly Val Gly Asp Gly Tyr 885 890 895 Gln Lys Ala Leu Asn Asn Tyr Ser Lys Gly Arg Thr Phe Val Leu Asn 900 905 910 Val Asn Tyr Lys Phe 915 70902PRTMoraxella catarrhalis 70Met Met Ile Thr Gly Asn Thr Met Asn Gln Ile Phe His Leu Met Thr 1 5 10 15 Gln Thr Lys His Thr Asn His Thr Lys Lys Val Leu Lys Leu Ser Met 20 25 30 Leu Ser Leu Cys Leu Leu His Ile Thr Gln Thr Ala Met Ala Glu Asp 35 40 45 Thr Leu Lys Asp Val Pro Lys Ala Thr Asp Phe Ser Val Ile Leu Asp 50 55 60 Glu Val Val Val Thr Ala Thr Asn Gly Thr Lys Lys Ser Gln Lys Pro 65 70 75 80 Phe Thr Lys Ala Ser Ala Thr Ser Val Arg Glu Asn Val Phe Asn Ala 85 90 95 Ser Glu Asn Ile Asp Ala Ile Val Arg Ser Val Pro Gly Ala Phe Thr 100 105 110 Gln Gln Asp Lys Ser Ser Gly Leu Val Ser Leu Asn Val Arg Gly Asp 115 120 125 Ser Gly Phe Gly Arg Ala Asn Ser Met Val Asp Gly Val Thr Gln Thr 130 135 140 Phe Tyr Ser Thr Ser Thr Asp Ala Gly Arg Gly Gly Gly Thr Ser Gln 145 150 155 160 Phe Gly Ala Val Ile Asp Gln Asn Phe Ile Ala Gly Val Glu Leu Asn 165 170 175 Lys Gly Ser Phe Asn Gly Lys Gly Gly Leu Asn Thr Leu Thr Gly Ser 180 185 190 Ala Asn Phe Arg Thr Leu Asn Ala Asp Asp Val Ile Lys Asp Asp Lys 195 200 205 Asn Phe Gly Phe Ile Ala Lys Gly Leu Thr Gly Lys Asn Ala Thr Asp 210 215 220 Lys Asn Phe Met Leu Ala Ala Gly Gly Arg Gly Trp Leu Asp Asn Gly 225 230 235 240 Ser Ile Ser Ala Leu Tyr Ala Tyr Ser His Lys Asp Ile Ser Gln Asn 245 250 255 Tyr Lys Val Gly Gly Gly Gly Thr His Ile Gly Asn Val Gly Asp Asp 260 265 270 Leu Leu Leu Ser Lys Gln Lys Gln Val Phe Ala Lys Glu His Ala Leu 275 280 285 Thr Tyr Asn Glu Ala Ser Arg Ser Trp Gln Lys Asp Leu Thr Lys Leu 290 295 300 Asp Lys Glu Thr Gly Lys Pro Leu Trp Asp Arg Lys Tyr Gln Phe Gly 305 310 315 320 Gly Lys Cys Tyr Gly Leu Gly Cys Ile Asp Thr Lys Glu Lys Phe Asp 325 330 335 Glu Tyr Val Ala Asp Lys Gln Gln Gln Trp Gln Lys His Gly Ala Lys 340 345 350 Glu Tyr Ser Ile Thr Pro Ile Asp Ile Thr Ala Leu Asn Gln Thr Ser 355 360 365 Lys Ser His Leu Ala Lys Ile Arg Tyr Asn Asn Asp Thr Ser Asp Val 370 375 380 Gly Leu Gln Leu Arg Lys Met Asp Thr Thr Ile Gly Ser Arg Arg Ile 385 390 395 400 Ser Asn Asp Asn Tyr Gln Leu Asp Ala Ala Tyr Asn Pro Asn Glu Ile 405 410 415 Ile Asp Leu Lys Val Leu Ala Ala His Asn Val Gly Val Gln Lys Tyr 420 425 430 Pro Lys Gly Ser Thr Phe Thr Gly Trp Lys Leu Asp Lys Asp Phe Glu 435 440 445 Thr Lys Asn Thr Ala Asn Leu Phe Asp Leu Asn Asn Thr His Thr Phe 450 455 460 Asn Leu Pro Lys Gln Met Asp Leu Thr Thr Thr Val Gly Leu Asn Ile 465 470 475 480 Leu His Asn Glu Tyr Ser Lys Asn Arg Phe Pro Asp Glu Leu Gly Leu 485 490 495 Phe Tyr Thr Asn Asp Leu Leu Cys Gly Gly Gly Tyr Asp Ala Cys Gly 500 505 510 Gly Arg Phe Gln Gly Thr Ser Ser Thr Leu Pro Lys Lys Ser Val Ile 515 520 525 Val Gln Pro Ser Gly Lys Gln Arg Phe His Ser Ile Tyr Leu Asp Thr 530 535 540 Ser Leu Gln Lys Asp Lys Tyr Gln Leu Asp Tyr Ser Val Asn Ala Ser 545 550 555 560 Gln Tyr Arg Phe Ser Gly Glu His Ala Ser Tyr Tyr Ser Ser Gln Lys 565 570 575 Glu Phe Gln Asp Lys Phe Gly Glu Asp Ser Gln Ile Tyr Lys Gln His 580 585 590 Cys Ser Pro Ser Cys Asp Val Tyr Glu Pro Leu Val Thr Thr Ser Gly 595 600 605 Lys Lys His Ala Ile Asn His Ser Val Thr Leu Ser Ala Lys Tyr Asp 610 615 620 Thr Gly Phe Met Pro Phe Val Ser Phe Ala Arg Thr His Arg Met Pro 625 630 635 640 Asn Ile Gln Glu Met Phe Phe Ser Gln Ile Gly Asp Val Gly Val Asn 645 650 655 Thr Ala Leu Lys Pro Glu Gln Ala Asn Thr Tyr Gln Leu Gly Phe Asn 660 665 670 Val Phe Lys Arg Asn Leu Leu Thr Asp Asn Asp Thr Leu Gly Leu Lys 675 680 685 Val Val Gly Tyr Gln Ser Arg Ile Asn Asn Tyr Ile His Asn Val Tyr 690 695 700 Gly Lys Trp Tyr Asp Thr Lys Asn Pro Pro Ser Trp Val Thr Ser Gly 705 710 715 720 Ala Leu Lys Gly Asp Thr Ile Gln His Arg Asn Trp Gln Met Pro Val 725 730 735 His Lys Gln Gly Leu Glu Leu Glu Ile Asn Tyr Asp Ala Gly Arg Tyr 740 745 750 Phe Thr Asn Leu Ser Tyr Ala Arg Gln Lys Thr Asp Gln Pro Thr Asn 755 760 765 Tyr Ser Asp Ala Ser Glu Ser Pro Arg Asn Ser Ser Lys Glu Asp Gln 770 775 780 Leu Thr Gln Gly Tyr Gly Leu Ser Lys Val Ser Met Leu Pro Lys Asp 785 790 795 800 Tyr Gly Arg Phe Glu Leu Gly Val Arg Gly Phe Asp Asp Lys Leu Thr 805 810 815 Ile Gly Ser Ala Val Arg Tyr Tyr Gly Gln Ser Pro Arg Ala Thr Ile 820 825 830 Glu Pro Arg Tyr Ile Asp Gly Thr His Gly Gly Asn Thr Ser His Ser 835 840 845 Asp Asp Lys Gly Ala His Val Ile Lys Gln Ile Glu Met Leu Lys Arg 850 855 860 Gln Pro Leu Val His Asp Phe Tyr Val Ala Tyr Glu Pro Ile Lys Asp 865 870 875 880 Leu Val Met Arg Leu Asp Val Gln Asn Ala Phe Asp Lys Leu Tyr Ile 885 890 895 Asp Pro Leu Asp Ala Asn 900 71860PRTPasteurella dagmatis ATCC 43325 71Met Asn Lys Ser Arg Lys Gly Leu Ser Tyr Ile Leu Pro Leu Ile Ile 1 5 10 15 Pro Phe Cys Ala Tyr Ala Glu Thr Thr Glu Leu Asp Val Ile Ser Val 20 25 30 Glu Thr Glu Ala Phe Lys Glu Lys Asp Lys Val Phe Leu Lys Gln Ser 35 40 45 Ala Ser Ser Thr Arg Thr Asn Phe Glu Thr Ser Thr Gln Ser Leu Asp 50 55 60 Thr Val Leu Arg Ser Val Pro Gly Ala Phe Thr Gln Ile Asp Lys Ser 65 70 75 80 Ser Gly Thr Val Ser Val Asn Val Arg Gly Gly Thr Gly Phe Gly Arg 85 90 95 Val Asn Ser Met Ile Asp Gly Val Ser Gln Thr Phe Tyr Ala Ser Ser 100 105 110 Ala Asp Gly Gly Ser Arg Ser Ser Ser Thr Ser Gln Phe Gly Asn Leu 115 120 125 Ile Asp Pro Gly Phe Leu Asn Ser Val Asp Ile Asp Arg Gly Ser Phe 130 135 140 Glu Gly Ser Ser Gly Ala Asn Thr Leu Leu Gly Ser Ala Asn Phe Lys 145 150 155 160 Thr Ile Gly Val Lys Asp Leu Val Thr Glu Gly Arg Gln Ile Gly Phe 165 170 175 Met Gly Lys Tyr Leu Trp Gly Ser Asn Ala Thr Lys Pro Gln Ile Leu 180 185 190 Gly Ala Val Ala Phe Lys Arg Asp Phe Glu Lys Glu Arg Trp Ile Gly 195 200 205 Leu Leu Tyr Gly Phe Ser Glu Arg His Leu Ser Gln Asp Tyr Arg Ile 210 215 220 Gly Gly Gly Arg Lys Val Thr Glu Ser Ser Ile Asp Leu Thr Gly Leu 225 230 235 240 Asp Glu Asp Asp Ile Glu Gln Thr Asp Thr Ser Pro Phe Asp Ala Ala 245 250 255 His Val Arg Gln Arg Pro Ile Ser His Leu Ala Lys Leu Glu Tyr Gly 260 265 270 Asp His Tyr Gln Gln Ser Thr Leu Ser Tyr Arg Gln Tyr Gln Thr Gly 275 280 285 Val Gly Gly Arg Ser Ile Gln Asn Asn Asn Tyr Gln Val Asn Tyr Asn 290 295 300 Phe Thr Leu Pro Asp Ser Ser Trp Leu Asp Phe Asn Phe Leu Ala Ala 305 310 315 320 Arg Asn Glu Ser Ser Gln Lys Tyr Ala Lys Gly Ala Met Ile Ile Gly 325 330 335 Lys Pro Leu Leu Glu Pro Leu Thr Val Asn Asn Lys Ala Asp Thr Ile 340 345 350 Asp Ile Asn Asn Thr Phe Arg Phe Glu Leu Pro Phe Glu Thr Ala Leu 355 360 365 Lys Thr Arg Ile Gly Leu Thr Thr Leu Lys Ser Arg Tyr Phe Lys Asn 370 375 380 Arg Asp Pro Ser Glu Glu Leu Asn Ile Asn Leu Glu Asp Glu Asp Lys 385 390 395 400 Pro Tyr Asp Phe Asp Cys Phe Gly Leu Gly Cys Val Arg Lys Ser Leu 405 410 415 Gly Lys Ala Thr Phe Gln Pro Asn Gly Lys Gln Asn Ile Ile Thr Phe 420 425 430 Tyr Ile Asp Asn Asn Leu Ser Trp Lys Ile Leu Ser Phe Asp Tyr Asn 435 440 445 Val Asn Leu Ser Arg Tyr Ser Leu Lys Gly Glu Arg

Leu Lys Tyr Leu 450 455 460 Pro Gln Tyr Ile Ser Asp Thr Ser Ala Glu Ile Asn Val Leu Ser Arg 465 470 475 480 Lys Ile Ala Arg Glu Lys Asn Pro Glu Val Arg Lys Gln Leu Gln Thr 485 490 495 Gln Ile Gln Glu Leu Glu Ser Lys Leu Ala Tyr Ile Lys Gln His Asn 500 505 510 Cys Val Arg Thr Tyr Asp Glu Asp Leu Asp Glu Tyr Leu Glu Glu Cys 515 520 525 Arg Glu Val His Phe Thr Leu Pro Asp Ser Ser Ala Gly Lys Met Tyr 530 535 540 Asn Tyr Ser Ala Thr Leu Ser Ala Asn Ile His Asp Leu Phe Thr Pro 545 550 555 560 Phe Ile Ser Tyr Ser Lys Ser His Arg Ala Pro Asn Ile Arg Glu Val 565 570 575 Phe Phe Ser Ser Val Ser Asp Tyr Gly Val Asn Thr Asn Leu Arg Pro 580 585 590 Glu Lys Ala Lys Val Ile Gln Phe Gly Ile Asn Gly Tyr Gln Glu Lys 595 600 605 Val Phe Thr Asp Asn Asp Lys Leu Gly Tyr Lys Val Val Tyr Tyr Asn 610 615 620 Thr His Val Lys Asp Phe Ile Tyr Asn Ser Asp Asn Arg Gln Pro Leu 625 630 635 640 Val Ala Gly Lys Leu Arg Tyr Leu Asn Asp Thr Asn Ile Leu His Lys 645 650 655 Asn Tyr Gln Gln Arg Val Lys Met Thr Gly Ile Glu Thr Glu Leu Ser 660 665 670 Tyr Asp Leu Gly Asn Ile Tyr Phe Asn Leu Ala Tyr Ala Arg Gln Lys 675 680 685 Asn Asn Gln Pro Val Ser Phe Thr Asp Gly Ser Ala Arg Gly Lys Gly 690 695 700 Ala Ser Glu Thr Glu Arg Leu Leu Gln Gly Phe Gly Ala Ser Lys Ile 705 710 715 720 Ser Ile Leu Pro Lys Asp Tyr Gly Ser Leu Glu Ile Gly Thr Arg Phe 725 730 735 Leu Asp Gly Lys Leu Asn Phe Ser Gly Thr Ala Lys Tyr Tyr Gly Ser 740 745 750 Ser Lys Arg Val Leu Ser Lys Pro Leu Thr Ile Lys Ser Gly Asp Ala 755 760 765 Arg Asp Thr Val Lys Glu Arg Ile Arg Val Thr Glu Glu Ile Pro Lys 770 775 780 Gln Pro Ile Ile Val Asp Leu Gln Val Ser Tyr Glu Pro Leu Lys Asn 785 790 795 800 Leu Val Leu Lys Ala Glu Val Gln Asn Val Phe Asp Lys Arg Tyr Val 805 810 815 Asn Pro Leu Asp Ser Asn Asn Asp Ala Ala Ser Gln Ser Val Phe Asn 820 825 830 Leu Asp Leu Ser Asp Gln Thr Ile Asn Val Leu Asn Asn Phe Ala Arg 835 840 845 Gly Arg Thr Tyr Val Phe Thr Val Ser Tyr Lys Tyr 850 855 860 7223DNAEscherichia colimisc_feature(12)..(12)n is a, c, g, or t 72gaaatgttat antataacat ttc 237323DNANeisseria meningitidis 73gtaatgttat ataataacaa act 237423DNANeisseria meningitidis 74caaacgttat acagtatcat atc 237530DNAArtificial Sequenceprimer sequence 75gatcatatgc atgaaactga gcaatcggtg 307633DNAArtificial Sequenceprimer sequence 76gatggatcct taaatcttca cgttcacgcc gcc 337726DNAArtificial Sequenceprimer sequence 77tgacgcgtct cgacgctgag gtctgc 267828DNAArtificial Sequenceprimer sequence 78tgtgtacagt cgacttcaga cggccacg 287931DNAArtificial Sequenceprimer sequence 79gcatcatatg gcacaaacta cactcaaacc c 318032DNAArtificial Sequenceprimer sequence 80atgacgtctt aaaacttcac gttcacgccg cc 328120DNAArtificial Sequenceprimer sequence 81gtacgatgat tgtgccgacc 208240DNAArtificial Sequenceprimer sequence 82actttaaact ccgtcgacgc aagtcgactg cgggggttaa 408340DNAArtificial Sequenceprimer sequence 83ttaacccccg cagtcgactt gcgtcgacgg agtttaaagt 408421DNAArtificial Sequenceprimer sequence 84gccatactgt tgcggatttg a 218520DNAArtificial Sequenceprimer sequence 85ttcgccgatg gcggaataca 208640DNAArtificial Sequenceprimer sequence 86ctttcagcgc aaagtcgact ccgtcgacgc gtgcctgttc 408740DNAArtificial Sequenceprimer sequence 87gaacaggcac gcgtcgacgg agtcgacttt gcgctgaaag 408820DNAArtificial Sequenceprimer sequence 88tcctattgcg caataccccc 208967PRTNeisseria meningitidis 89Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 1 5 10 15 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 20 25 30 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 35 40 45 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asn Ala His Ala His 50 55 60 Thr His Ser 65 90758PRTNeisseria meningitidis MC58 90Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Glu Gln Ser Val Asp 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Val Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asn Ala His Ala His 305 310 315 320 Thr His Ser Gly Arg Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Ile Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Gln Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Phe His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 91758PRTNeisseria meningitidis O53322 91Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Glu Gln Ser Val Gly 20 25 30 Leu Glu Thr Val Thr Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Lys Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Ala Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Val Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asn Ala His Ala His 305 310 315 320 Thr His Asn Gly Lys Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Leu Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr His His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Lys Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Ile Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Glu Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Gln Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Lys Gln Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Val His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 92758PRTNeisseria meningitidis Z2491 92Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ser Gln Ala His Gly Thr Glu Gln Ser Val Gly 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser

35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Ser Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Ala Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asp Ala His Ala His 305 310 315 320 Ala His Asn Gly Lys Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gly Gln Lys Ser Ser Ala Leu Ser Ala Thr Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Arg Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Leu Ile Ala Gln Ala Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Val His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 93758PRTNeisseria meningitidis FAM18 93Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Glu Gln Ser Val Asp 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Leu Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Val Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asp Ala His Ala His 305 310 315 320 Thr His Ser Gly Arg Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Ile Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Arg Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Val His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 94758PRTNeisseria meningitidis alpha14 94Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Glu Gln Ser Val Asp 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Val Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Val Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asp Ala His Ala His 305 310 315 320 Ala His Asn Gly Lys Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Thr Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Arg Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Val His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 95758PRTNeisseria meningitidis alpha153 95Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Asp Arg Ser Val Asp 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50

55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Ala Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asp Ala His Ala His 305 310 315 320 Ala His Asn Gly Lys Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Thr Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Arg Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Val His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 96728PRTNeisseria meningitidis alpha 275 96Val Asp Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala 1 5 10 15 Thr Ser Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser 20 25 30 Gly Asp Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp 35 40 45 Gly Val Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala 50 55 60 Pro Val Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His 65 70 75 80 His Gly Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile 85 90 95 Met Val Asp Ser Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro 100 105 110 Val Thr Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val 115 120 125 Ala Asp Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly 130 135 140 Glu Leu Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser 145 150 155 160 Gly Gly Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu 165 170 175 Gly Leu Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn 180 185 190 Leu Lys Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile 195 200 205 Gly Leu Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Ala Ala Tyr Ser 210 215 220 Asp Arg Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp 225 230 235 240 Asp Cys His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg 245 250 255 Tyr Leu Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr 260 265 270 Asp Asn Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asp Ala His 275 280 285 Ala His Ala His Asn Gly Lys Pro Trp Ile Asp Leu Arg Asn Lys Arg 290 295 300 Tyr Glu Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala 305 310 315 320 Leu Arg Val His Leu Asn Arg Asn Asp Tyr His His Asp Glu Lys Ala 325 330 335 Gly Asp Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg 340 345 350 Ile Glu Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly 355 360 365 Val Gln Tyr Leu Gly Gln Lys Ser Ser Ala Leu Ser Ala Thr Ser Glu 370 375 380 Ala Val Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser 385 390 395 400 Phe Phe Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly 405 410 415 Gly Val Arg Val Glu Lys Gln Lys Ala Ser Ile Arg Tyr Asp Lys Ala 420 425 430 Leu Ile Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly 435 440 445 Ala His Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr 450 455 460 Phe Thr Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg 465 470 475 480 Leu Pro Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr 485 490 495 Asn Thr Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn 500 505 510 Asn Ile Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn 515 520 525 Leu Ala Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr 530 535 540 Leu Asn Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met 545 550 555 560 Lys Leu Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu 565 570 575 Gly Glu Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser 580 585 590 Gly Asp Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly 595 600 605 Arg Glu Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln 610 615 620 Asn Ala Pro Arg Val Pro Ala Ala Arg Leu Gly Val His Leu Lys Ala 625 630 635 640 Ser Leu Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe 645 650 655 Ala Gln Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His 660 665 670 Met Leu Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu 675 680 685 Trp Asn Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr 690 695 700 Ala His Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe 705 710 715 720 Thr Gly Gly Val Asn Val Lys Phe 725 97803PRTAcinetobacter baumannii 97Met Leu Asn Lys Ser Lys Leu Phe Leu Ala Leu Ile Thr Leu Gly Ala 1 5 10 15 Ser Lys Ile Leu Leu Ala Ala Glu Gly Pro Val Thr Thr Leu Asn Thr 20 25 30 Ile Val Leu Thr Ala Gln Ser Asp Glu Leu Gly Ser Glu Leu Leu Gly 35 40 45 Lys Ser Leu Asn Val Ser Asn Gln Phe Ile Asp Thr Ser Lys Leu Lys 50 55 60 Gln Arg Ser Thr Thr Leu Gly Asp Ala Leu Gly Thr Glu Leu Gly Ile 65 70 75 80 His Ser Asn Gln Tyr Gly Gly Gly Ala Ser Ala Pro Ile Ile Arg Gly 85 90 95 Gln Glu Gly Lys Arg Ile Lys Val Leu Gln Asn Asn Ala Asp Val Leu 100 105 110 Asp Met Ser Asn Met Ser Pro Asp His Ala Val Thr Val Glu Pro Ser 115 120 125 Leu Ala Lys Ser Ile Glu Ile Ile Arg Gly Ala Ser Thr Leu Leu Tyr 130 135 140 Ser Ser Asn Ser Ala Ala Gly Val Val Asn Val Ile Asp Tyr Lys Ile 145 150 155 160 Pro Thr Gln Met Pro Gln Asp Gly Leu Glu Gly Asn Thr Thr Leu Arg 165 170 175 Phe Asn Thr Gly Ser Asn Glu Lys Leu Thr Thr Ala Gly Val Thr Val 180 185 190 Gly Leu Ser Pro Arg Val Ala Leu Arg Ala Glu Gly Leu Tyr Arg Asn 195 200 205 Ala Gly Asn Tyr Lys Thr Pro His Tyr Gln Ser Ser Ser Tyr Asn Ser 210 215 220 Leu Glu Asp Leu Glu Asn Gln Asn Ile Val Tyr Lys Asn Leu Lys Tyr 225 230 235 240 Leu Pro Glu Ser Trp Ala Glu Ser Arg Leu Gly Thr Leu Gly Leu Ser 245 250 255 Trp Ile Asp Asp Asn Thr Tyr Leu Gly Val Ser Tyr Thr His Arg His 260 265 270 Asp Glu Tyr Gly Leu Pro Ala His Ser His Leu Tyr Glu Gly Cys Gly 275 280 285 Ala Ser Ala Ile Ser Ile Asn Thr Arg Ile Ser Gly Leu Lys Asn Tyr 290 295 300 Leu Leu Tyr Tyr Pro Gln Leu Met Glu Glu Gln Asp Ile Asn Tyr Val 305 310 315 320 Asn Pro Arg Pro Asp Cys His Gln His Asn His Ile His Glu Thr Thr 325 330 335 Phe Ser His Asn Ala Pro Tyr Ile Asp Leu Asn Thr Arg Arg Tyr Asp 340 345 350 Met Arg Gly Glu Phe Thr Gln Pro Phe Thr Gly Ile Asp Lys Ile Arg 355 360 365 Thr Ser Leu Ser Tyr Ile Asp Tyr Phe His Asn Glu Leu Glu Gly Asp 370 375 380 Lys Ile Thr Asn Phe Phe Lys Asn Thr Gly Lys Val Gly Arg Ile Glu 385 390 395 400 Leu Ser His Gln Pro Leu Gly Glu Leu Thr Gly Ile Leu Gly Leu Gln 405 410 415 Tyr Leu Glu Gln Asp Asn Ser Ala Leu Ser Pro Val His Ser Gln Glu 420 425 430 Gly His Thr Thr Tyr Leu Asp Thr Gln Gln Leu Leu Asn Arg Asn Val 435 440 445 Thr Lys Asn Phe Ser Val Phe Gly Leu Glu Lys Tyr Asn Trp Asn Asp 450 455 460 Phe Thr Phe Glu Leu Gly Ala Arg Ile Glu Lys Gln Lys Val Ser Met 465 470 475 480 Asp Tyr Asp Ile Glu Lys Ile Lys Asp Ser Met Lys Pro Trp Pro Asn 485 490 495 Lys Tyr Asn Ser Pro Tyr Val Glu Lys Asn Asn Lys Ile Arg Ala Gln 500 505 510 Asn Leu Lys Ser Ile Leu Glu Ala Val Gln Pro Asn Lys Glu Thr Ala 515 520 525 Phe Ser Tyr Ala Gly Thr Val His Trp Arg Phe Ala Pro Asn Tyr Ile 530 535 540 Leu Ser Leu Thr Gly Thr His Gln Glu Arg Leu Pro Asn Ala Gln Glu 545 550 555 560 Met Tyr Thr His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly 565 570 575 Asn Arg Phe Leu Arg Lys Glu Lys Ser Asn Asn Leu Glu Ile Ser Leu 580 585 590 Ala Tyr Lys Asp Asp Leu Leu Asp Tyr Gln Ile Ser Thr Tyr Tyr Tyr 595 600 605 Asp Phe Asp Asn Tyr Ile Tyr Leu Gln Thr Leu Asn Glu Val Leu Gly 610 615 620 Thr Thr Lys Val Arg Asp Gln His Thr Leu Arg Ile Asn His Tyr Ser 625 630 635 640 Gln Ser Ala Ala Asn Phe Tyr Gly Leu Glu Gly Asn Ile Gly Tyr Gln 645 650 655 Phe Asn Ser Val Tyr His Gly Ser Leu Phe Gly Asp Tyr Val Lys Gly 660 665 670 Arg Leu Thr Asn Leu Pro Asp Ala Val Ile Ala Tyr Asp Ile Trp Asn 675 680 685 Arg Glu Pro Thr Leu Ala Pro Gln Lys Asp Arg Tyr Thr Pro Arg Leu 690 695 700 Pro Pro Ala Arg Leu Gly Thr Arg Leu Lys Ala Asp Phe Asp Glu Ser 705 710 715 720 Leu Lys Gly Glu Ile Glu Tyr Tyr Arg Val Phe Lys Gln Asp Asn Ile 725 730 735 Ser Lys Phe Glu Gln Val Thr Ser Gly Tyr Asn Met Leu Asn Met Thr 740 745 750 Leu Ala Tyr Lys Asn Lys Leu Ser His Thr Glu Tyr Asp Leu Phe Phe 755 760 765 Lys Ala Asn Asn Leu Leu Asp Gln Lys Val Tyr Ala His Glu Thr Phe 770 775 780 Leu Pro Tyr Ile Pro Gln Ile Gly Arg Asn Phe Ser Leu Gly Leu Asn 785 790 795 800 Leu Asn Phe 98790PRTActinobacillus pleuropneumoniae 98Met Phe Asn Lys Lys Leu Leu Ala Val Leu Ile Ser Ala Gln Phe Ser 1 5 10 15 Pro Leu Val Trp Ala Asn Asn Asn Asp Val Ala Val Leu Asp Glu Val 20 25 30 Ser Val Val Gly Ser Thr Pro Ser Ile Ser Gln Gly Ser Glu Val Thr 35 40 45 Leu Leu Lys Val Ser Asp Lys Ile Ile Ala Gly Lys Glu Phe Lys Lys 50 55

60 Arg Ser Ala Thr Leu Gly Asn Ala Leu Ala Ala Glu Leu Gly Val His 65 70 75 80 Ser Asn Pro Phe Gly Gly Gly Ala Ser Lys Pro Ile Ile Arg Gly Gln 85 90 95 Glu Gly Ala Arg Ile Arg Ile Leu Gln Asn Gly Ser Asp Val Ile Asp 100 105 110 Met Ser Asn Leu Ser Pro Asp His Ala Val Val Ala Asp Ser Leu Leu 115 120 125 Ala Lys Gln Val Glu Ile Leu Arg Gly Ser Ser Thr Leu Leu Tyr Ala 130 135 140 Ser Ser Ser Pro Ala Gly Ile Val Asn Val Val Asp Lys Arg Ile Pro 145 150 155 160 Thr Glu Ile Pro Glu Lys Gly Tyr Glu Val Glu Leu Asn Ser Arg Phe 165 170 175 Asp Thr Ala Ala Lys Glu Lys Val Gly Ala Leu Gly Ala Thr Phe Gly 180 185 190 Ile Gly Lys His Ile Ala Val Arg Ala Glu Gly Leu Thr Arg His Ser 195 200 205 Asp Asn Tyr Arg Val Pro Gly Ile Asn Leu Gly Glu Arg Leu Asn Tyr 210 215 220 Val Pro Asp Thr Tyr Asn Lys Ser Lys Val Gly Thr Leu Gly Leu Ser 225 230 235 240 Phe Val Gly Glu Gln Gly Tyr Ile Gly Ala Ser Tyr Ser Lys Arg Arg 245 250 255 Asp Asn Tyr Gly Leu Pro Gly His Asn His Lys Phe Asp Phe Cys Ile 260 265 270 Gly His Ile Tyr Gly Asn Lys Gln Gly Lys Tyr Ala Tyr Thr Tyr Leu 275 280 285 Tyr Pro His Leu Ile Gly Glu Glu Asn Ile Gly Ser Asn Pro His Phe 290 295 300 His Cys Gly Thr Asp His Ala Glu Asp Gly Thr His Ser His Asp Asn 305 310 315 320 Pro Phe Gly His Asp His Asp His Thr His Pro Gly Pro Trp Val Asp 325 330 335 Leu Glu Ser Lys Arg Phe Asp Val Lys Ala Glu Leu Arg Gln Pro Phe 340 345 350 Lys Gly Ile Asp Lys Ile Lys Val Ser Tyr Ala Asp Ala Asp Tyr Tyr 355 360 365 His Asp Glu Lys Asp Ala Gly Val Leu Ala Thr Arg Tyr His Lys Gln 370 375 380 Leu Lys Lys Asp Gln Asp Tyr Gly Lys Pro Val Asn Ile Phe Lys Asn 385 390 395 400 Arg Gly Lys Asn Ala Arg Leu Glu Ile Tyr His Ala Pro Leu Gly Gly 405 410 415 Leu Thr Gly Val Trp Gly Val Gln Tyr Gln Thr Gln Lys Ser Ser Met 420 425 430 His Ala Pro Lys Asp Arg Glu Val Lys Phe Pro Leu Val Glu Asn Thr 435 440 445 Asn Lys Gln Met Ser Leu Phe Gly Ile Glu Gln Tyr Met Trp Asp Asn 450 455 460 Phe Ala Leu Glu Phe Ala Gly Arg Val Glu Lys Gln Lys Ile Glu Ile 465 470 475 480 Glu Tyr Asp Arg Asn Glu Ile Lys Arg Leu Gln Asp His Tyr Arg Ile 485 490 495 Ser Gly Gly Lys Gln Val Glu Pro Asp Leu Ser Pro Tyr Asn Gln Asn 500 505 510 Ala Tyr Ala Tyr Ser Ser Thr Leu Asn Trp Phe Phe His Pro Asp Tyr 515 520 525 Gln Leu Ser Phe Thr Ala Ser His Asn Glu Arg Phe Pro Thr Pro Met 530 535 540 Glu Leu Tyr Tyr His Gly Gln His Ile Ala Thr Asn Ser Phe Glu Tyr 545 550 555 560 Gly Asn Lys Asp Leu Lys Lys Glu Gln Ser Asn Asn Val Glu Leu Gly 565 570 575 Leu Gly Tyr Gln Thr Glu Arg Val Gly Tyr Lys Val Asn Val Tyr Tyr 580 585 590 Asn His Phe Lys Asn Tyr Ile Tyr Asn Glu Asn Leu Phe Arg Glu Asn 595 600 605 Gln Leu Phe Met Arg Arg Tyr Asn Gln Ala Lys Ala Arg Phe Tyr Gly 610 615 620 Ile Glu Ala Glu Ala Ser Tyr Arg Phe Asn Asp Lys Tyr Gln Ala Thr 625 630 635 640 Ile Phe Gly Asp Met Val Arg Gly Trp Leu Thr Asn Leu Pro Pro Leu 645 650 655 Thr Val Asn Ser Asp Tyr Ser Val Phe Lys Asp Tyr Leu Pro Lys Asp 660 665 670 Ala Lys Pro Gly Glu Asp Tyr Leu Ile Tyr Arg Ala Asp Gln Asn Thr 675 680 685 Pro Arg Thr Pro Pro Val Arg Leu Gly Phe Arg Phe Asn Ala Glu Phe 690 695 700 Thr Pro Asn Trp Ser Gly Asp Leu Glu Leu Ile Arg Thr Phe Thr Gln 705 710 715 720 Arg Arg Thr Ser Gln Leu Glu Tyr Ile Thr Glu Gly Asn Thr Met Leu 725 730 735 Asn Ile Gly Val Ala Tyr Ser Asn Lys Trp Lys Asp Leu Asp Tyr Lys 740 745 750 Ile Ser Leu Asn Gly Thr Asn Leu Leu Asn Gln Pro Val Tyr Ile His 755 760 765 Thr Ser Tyr His Gln Phe Val Pro Gln Thr Gly Arg Asn Phe Ile Leu 770 775 780 Val Val Asn Val Lys Phe 785 790 99809PRTHaemophilus parasuis 99Met Ile Asn Asn Arg Thr Thr Glu Gln Gln Asn Asn Arg Thr Thr Ala 1 5 10 15 Phe Ser Leu Ala Phe Ser Leu Leu Leu Cys Cys Leu Gly Ile Asn Ala 20 25 30 Glu Gln Leu Glu Leu Asp Glu Ile Ser Val Met Gly Lys Val Pro Glu 35 40 45 Gly Asn Ser Ile Ser Phe Leu Lys Val Ser Asp Ala Ile Ile Asp Gly 50 55 60 Glu Lys Phe Lys Asn Arg Ser Ala Thr Leu Gly Asn Ala Leu Ser Ser 65 70 75 80 Glu Leu Gly Val His Ser Thr Pro Phe Gly Gly Gly Ala Ser Ala Pro 85 90 95 Ile Ile Arg Gly Gln Glu Gly Val Arg Val Lys Ile Leu Gln Asn Asn 100 105 110 Ala Asp Val Val Asp Met Ser Asn Ile Ser Pro Asp His Ala Ile Thr 115 120 125 Ala Asp Thr Leu Leu Ala Asn Gln Val Glu Ile Leu Arg Gly Ala Ser 130 135 140 Thr Leu Leu Tyr Ala Ser Ser Ser Pro Ala Gly Ile Val Asn Ile Val 145 150 155 160 Asp Gln Arg Ile Pro Asn Lys Met Pro Lys Lys Gly Tyr Glu Val Thr 165 170 175 Leu Ser Ser Arg Phe Asp Thr Ala Ser Lys Glu Lys Val Tyr Ala Leu 180 185 190 Gly Thr Thr Ile Gly Ile Gly Lys His Leu Ala Leu Arg Leu Glu Gly 195 200 205 Leu Asp Arg Gln Ser Gln Asn Tyr Lys Val Pro Gln Ile Lys Leu Gly 210 215 220 Glu Thr Leu Asn Tyr Val Pro Asp Thr Tyr His Gln Ser Lys Val Gly 225 230 235 240 Thr Ile Gly Leu Ser Phe Ile Gly Glu Lys Gly Tyr Leu Gly Ala Ser 245 250 255 Tyr Asn Gln Arg Lys Asp Arg Tyr Gly Leu Pro Gly His Asn His Lys 260 265 270 Phe Asp Thr Cys Ile Ala His Ile Tyr Asp Met Arg Leu Gln Gly Lys 275 280 285 His Ser Tyr Thr Asn Leu Tyr Pro His Leu Met Ser Asp Glu Met Val 290 295 300 Thr Glu Asn Pro His Phe His Cys Gly Thr Asp Tyr Asp Leu Asp Pro 305 310 315 320 Ser His Ser His Asp His Pro Tyr Gly His Asp His Asp His Thr His 325 330 335 Ile Gly Pro Trp Val Asp Leu His Ser Lys Arg Ile Asp Ile Lys Gly 340 345 350 Glu Ile Lys Gln Pro Leu Pro Met Leu Asp Lys Ile Gln Leu Ser Tyr 355 360 365 Ala Gln Thr Asp Tyr Tyr His Asp Glu Lys Asp Ala Gly Lys Ser Gly 370 375 380 Asp Thr Ile Asn Pro Asn Arg Val Asp Lys Ser Lys Asp Phe Gly Lys 385 390 395 400 Pro Val Asn Ile Phe Lys Asn Gln Gly Lys Asn Ala Arg Leu Glu Phe 405 410 415 Phe His Thr Pro Ile Gly Gly Leu Thr Gly Met Phe Gly Val Gln Tyr 420 425 430 Gln Thr Leu Gln Ser Ser Ala Asn Thr Pro Asn Asn Arg Glu Val Gln 435 440 445 Trp Pro Leu Val Asp Asn Arg Asn Lys Gln Ile Ser Leu Phe Ala Leu 450 455 460 Glu Gln Tyr Ala Trp Asp Asn Phe Ala Ile Glu Leu Gly Leu Arg Thr 465 470 475 480 Glu Lys Gln Asn Ile His Ile Asp Tyr Asp Leu Ala Lys Ile Gln Lys 485 490 495 Gln Gln Lys Phe Asn Glu Arg Thr Tyr Gly Lys Gln Val Asp Pro Asp 500 505 510 Leu Ser Asp Tyr Asp Glu Lys Ala Ile Ser Tyr Thr Gly Ala Phe Asn 515 520 525 Trp Phe Phe His Pro Asp Tyr Gln Leu Ser Phe Thr Ala Ser His Asn 530 535 540 Glu Arg Leu Pro Thr Pro Met Glu Leu Tyr Tyr His Gly Gln His Leu 545 550 555 560 Ala Thr Asn Ser Phe Glu Tyr Gly Asn Lys Asp Leu Lys Lys Glu Ile 565 570 575 Ser Asn Asn Phe Glu Leu Gly Leu Gly Tyr His Thr Glu Lys Leu Asp 580 585 590 Tyr Lys Leu Ser Thr Tyr Tyr Asn Asn Phe Asp Asn Tyr Ile Tyr Asn 595 600 605 Glu Thr Leu Tyr Arg Ser Asn Asn Leu Phe Met Arg Arg Tyr Asn Gln 610 615 620 Ala Lys Ala Thr Phe Tyr Gly Leu Glu Gly Ile Ile Asn Tyr Arg Phe 625 630 635 640 Thr Pro Asp Tyr Gln Phe Ser Val Phe Gly Asp Met Val Lys Gly Lys 645 650 655 Leu Lys Gln Leu Pro Asp Ile Lys Gly Leu Asn Asp Val Tyr Gly Glu 660 665 670 Pro Ile Leu Asn Pro Asp Tyr Asp Pro Glu Tyr Asp Glu Pro Glu Asp 675 680 685 Gln Tyr Tyr Arg Pro Tyr Leu Gly Lys Glu Met Ile Lys Gln Ala Asp 690 695 700 Arg Val Ser Pro Arg Leu Pro Pro Ile Arg Leu Gly Ala Arg Phe Asn 705 710 715 720 Ala Gln Leu Thr Glu Asn Leu Ser Gly Ser Val Glu Trp Met Lys Val 725 730 735 Phe Thr Gln Asn Lys Val Ser Lys Leu Glu Ser Ser Thr Lys Gly Tyr 740 745 750 Gln Leu Leu Asn Ala Ser Leu Asn Tyr Arg Arg Gln Ile Lys Gly Val 755 760 765 Glu Tyr Thr Val Ser Leu Thr Gly Asn Asn Leu Leu Asn Gln Ala Val 770 775 780 Tyr Ile His Asn Ser Tyr His Pro Tyr Val Pro Gln Met Gly Arg Asn 785 790 795 800 Phe Ile Leu Gly Leu Asp Leu Ser Phe 805 100693PRTBordetella pertussis 100Met Arg Phe Glu Arg His Pro Leu Ser Ala Ala Leu Ala Leu Ala Leu 1 5 10 15 Ala Trp Gln Gly Ala His Ala Gln Ala Ser Ala Asp Gly Thr Ser Glu 20 25 30 Ala Ala Thr Leu Ala Pro Ile Thr Val Ser Ala Ser Pro Leu Ala Gly 35 40 45 Asp Leu Asp Ser Met Thr Ala Pro Ala Ala Val Leu Glu Gly Asp Gln 50 55 60 Leu Leu Leu Arg Arg Gln Gly Thr Leu Gly Asp Thr Leu Asp Gly Leu 65 70 75 80 Pro Gly Val His Ala Asp Thr Phe Gly Gly Gly Ala Ser Arg Pro Val 85 90 95 Ile Arg Gly Gln Thr Ala Pro Arg Val Lys Val Leu Ser Asp Gly Ser 100 105 110 Glu Leu Met Asp Ala Ser Ala Ile Ser Pro Asp His Ala Val Thr Thr 115 120 125 Glu Pro Leu Leu Ala Asp Lys Ile Glu Val Leu Arg Gly Pro Ala Thr 130 135 140 Leu Leu Tyr Gly Gly Gly Ala Ile Gly Gly Val Val Asn Val Leu Asp 145 150 155 160 Arg Lys Ile Pro Thr Ala Val Pro Gln Gln Gly Ile Glu Ala Glu Ala 165 170 175 Glu Leu Arg Gly Ala Thr Gly Thr Lys Glu Arg Ala Gly Ala Ile Gly 180 185 190 Ile Thr Ala Gly Ser Gly Asn Phe Ala Val Arg Val Glu Gly Leu Lys 195 200 205 Arg Arg Ser Ser Asp Tyr Arg Val Pro Asp Trp Pro Asp Gly Lys Leu 210 215 220 Ala Gly Ser Tyr Ser Glu Ser Gly Gln Gly Thr Val Gly Met Ser Trp 225 230 235 240 Ile Thr Pro Arg Gly Tyr Val Gly Val Ala Phe Thr His Leu Glu Ser 245 250 255 Lys Tyr Gly Leu Pro Gly His Asn His Glu Tyr Glu Gly Cys His Pro 260 265 270 His Gly Ser His Leu His Cys Gly Gly His Asp Asp His Gly His Gly 275 280 285 His Asp Glu His Glu Glu Gly Glu Ala Glu His Asp His Gly His Glu 290 295 300 His Gly Ala Gly Asp Val Pro Tyr Val Lys Leu Arg Ser Asn Arg Thr 305 310 315 320 Asp Leu Arg Ala Glu Tyr Thr Asp Pro Phe Ala Gly Phe Glu Lys Ile 325 330 335 Arg Phe Arg Gly Gly Leu Thr Asp Tyr Arg His Asp Glu Ile Glu Gly 340 345 350 Gly Gln Leu Gly Thr Arg Phe Gln Asn Arg Gly Tyr Asp Ala Arg Leu 355 360 365 Glu Leu Thr His Arg Pro Leu Tyr Gly Trp His Gly Val Val Gly Val 370 375 380 Gln Thr Ser Tyr Ser Asp Phe Arg Ala Thr Gly Glu Glu Ala Phe Leu 385 390 395 400 Pro Arg Ser Lys Thr Arg Ala His Gly Leu Phe Leu Leu Glu Glu Tyr 405 410 415 Arg Trp Ala Asp Trp Arg Phe Glu Leu Gly Ala Arg Gln Asp Trp Gln 420 425 430 Arg Val Ser Pro Gln Ser Gly Ala Pro Ala Ser Arg Thr Ala Gly Thr 435 440 445 Ser Leu Ser Ala Ala Ala Ile Trp Asp Phe Ala Pro Gln Tyr Ser Leu 450 455 460 Ala Leu Ser Val Ser Arg Ser Gln Arg Leu Pro Ser Ala Gln Glu Leu 465 470 475 480 Tyr Ala Asp Gly Val His Leu Ala Thr Asn Thr Tyr Glu Ile Gly Asp 485 490 495 Pro Gly Leu Asp Arg Glu Thr Ser Arg Asn Val Asp Leu Thr Leu Arg 500 505 510 Lys His Ser Gly Asp Thr Thr Phe Ser Val Ser Ala Phe His Asn Arg 515 520 525 Val Lys Asn Tyr Ile Tyr Ala Asn Thr Leu Asp Arg Tyr Glu Asp Phe 530 535 540 Arg Leu Ile Glu Tyr Thr Gln Arg Asp Ala Glu Phe Thr Gly Val Glu 545 550 555 560 Gly Glu Val Arg His Arg Phe Gly Lys Val Phe Ser Ala Ala Val Phe 565 570 575 Gly Asp Tyr Val Arg Gly Arg Leu Thr Gly Gly Gly Gly Asn Leu Pro 580 585 590 Arg Ile Pro Ala Ala Arg Leu Gly Val Arg Ala Asp Ala Gln Trp Gln 595 600 605 Asn Trp Ala Gly Gly Val Glu Tyr Phe His Val Tyr Arg Gln Asp Asp 610 615 620 Ile Ala Ala Tyr Glu Ser Ser Thr Pro Gly Tyr Asp Met Val Asn Ala 625 630 635 640 Thr Ile Arg Tyr Arg Gly Lys Leu Asp Arg Thr Ala Tyr Glu Ile Tyr 645 650 655 Leu Arg Gly Asn Asn Leu Leu Asn Lys Leu Ala Phe Asn His Ala Ser 660 665 670 Phe Ile Ser Thr Val Ala Pro Leu Pro Gly Arg Ser Val Leu Leu Gly 675 680 685 Val Arg Leu Thr Tyr 690 101818PRTMoraxella catarrhalis 101Met Lys Val Thr Met Ile Lys Lys Pro Leu Ala Cys Ala Ile Leu Ala 1 5 10 15 Thr Phe Ser Met Pro Met Leu Ala Glu Ala Asn Leu Lys Asp Lys Pro 20 25 30 Thr Val Ile Leu Asp Gly Val Ser Ile Thr Ser Leu Ala Asp Gln Asn 35 40 45 Thr Glu Phe Gly Val Asn His Ser Lys Thr Val Ser Gly Ile Thr Val 50 55 60

Ser Lys Glu Gln Leu Gln Gln Arg Ala Thr Thr Leu Gly Asp Ala Leu 65 70 75 80 Ala Gly Glu Leu Gly Val His Ser Asn His Phe Gly Gly Gly Ala Ser 85 90 95 Ala Pro Ile Ile Arg Gly Gln Glu Gly Lys Arg Leu Lys Ile Leu Gln 100 105 110 Asn Gly Ser Glu Val Val Asp Met Ser Gly Leu Ser Pro Asp His Ala 115 120 125 Ile Ala Val Asp Thr Thr Leu Ala Lys Gln Val Glu Ile Val Arg Gly 130 135 140 Ser Gly Ala Leu Leu Tyr Ala Ser Gly Asn Ser Ala Gly Val Val Asn 145 150 155 160 Val Val Asp Asp Lys Ile Pro Ser Lys Leu Pro Ser Lys Leu Gln Gly 165 170 175 Asp Val Thr Val Arg Leu Ser Ser Ala Asn Arg Glu Lys Leu Ile Thr 180 185 190 Ala Ser Ala Glu Ala Pro Leu Gly Glu His Val Ala Val Arg Val Ala 195 200 205 Gly Leu Ser Lys Gln Ala Ala Asp Tyr Lys Thr Pro Arg Phe Asp Arg 210 215 220 His Val Phe Asn Lys Lys His Glu Asp Asp Asn Thr Gln Pro Glu Phe 225 230 235 240 Ile Tyr Lys Asp Thr Leu Lys His Leu Pro Asp Ser His Ala Lys Ser 245 250 255 Asn Ala Gly Thr Leu Gly Val Ser Trp Val Gly Asn Gln Gly Phe Leu 260 265 270 Gly Ala Ser Val Ser Leu Arg Arg Asp Lys Tyr Gly Leu Pro Asn His 275 280 285 Ser His Glu Tyr Glu Glu Cys Ser Val His Gly Ile Ser Gln Ser Ala 290 295 300 Leu Gln Tyr Lys Pro Tyr Leu Arg Leu Tyr Pro Phe Leu Met Glu Asn 305 310 315 320 Asp Asp Leu Glu Phe Asp Asn Ala Gly Leu Glu Cys His Thr His Asp 325 330 335 Asp His Asp His Glu His Asp His Ala His Asp His Glu His Asp His 340 345 350 Glu His Asp His Gly Lys Pro Trp Ile Asp Leu Lys Met Lys Arg Tyr 355 360 365 Asp Val Gln Gly Gln Ile Asn Ala Pro Phe Ala Gly Ile Asp Lys Ile 370 375 380 Arg Ala Ser Met Gly Lys Val Asp Tyr His His Asp Glu Ile Asp Gly 385 390 395 400 Gly Glu Lys Thr Ser Phe Phe Asp Asn Gln Ala Asn Val Trp Arg Leu 405 410 415 Glu Ala Ser His Thr Pro Ile His Thr Pro Met Gly Lys Phe Ser Gly 420 425 430 Val Phe Gly Val Gly Tyr Leu Thr Ser Lys Asn Ser Gly Leu Val Pro 435 440 445 Pro Arg Tyr Glu Asp Gly Asn Lys Gln Asp Thr Gln Asn Ile Leu His 450 455 460 Asn Asn Lys Thr Lys Thr Gly Ser Val Phe Trp Phe Glu Glu Tyr Lys 465 470 475 480 Pro Asn Asp Lys Leu Thr Val Asp Ala Ala Ala Arg Ile Glu Lys Gln 485 490 495 Thr Ile Thr Met Asp Tyr Asp Lys Asp Ala Ile Tyr Gln Ser Leu Asn 500 505 510 Leu Gly Leu Ala Thr Ala His Glu Pro Asp Ile Arg Phe Lys Arg Leu 515 520 525 Leu Asp Ser Gly Thr Leu Asn Pro Lys Lys Gln Thr Ala Arg Ser Tyr 530 535 540 Ala Val Gly Thr His Leu Gln Leu Thr Pro Lys His Lys Leu Ser Leu 545 550 555 560 Asn Leu Ser His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala 565 570 575 His Gly Met His Leu Ala Thr Asn Ser Phe Glu Ile Gly Asn Arg Phe 580 585 590 Leu Asn Lys Glu Lys Ser Asn Asn Ile Asp Leu Gly Leu Thr Phe Gln 595 600 605 Gly Asp Lys Trp Asp Tyr Arg Leu Gly Gly Tyr His Tyr Asp Phe Asp 610 615 620 Asn Tyr Val Phe Leu Gln Thr Leu Ser Gln Tyr Lys Gln Gly Leu Arg 625 630 635 640 Gly Met Arg His Asp Lys Asp Leu Lys Thr Ala Arg Tyr Glu Gln Ala 645 650 655 Ala Ala Lys Phe Tyr Gly Phe Asp Val Asn Ile Gly Tyr Gln Ile Asn 660 665 670 Asp Val Tyr His Val Ala Leu Phe Gly Asp Tyr Ile Arg Gly Lys Leu 675 680 685 Thr Asn Leu Pro Asp Lys Lys Gly Arg Thr Asp Ala Tyr Gly Asn Arg 690 695 700 Pro Leu Ile Lys Gln Pro Asp Ser His Thr Pro Arg Leu Pro Pro Lys 705 710 715 720 Arg Leu Gly Met Lys Leu Thr Ala Asn Val Asn Ala Asn Trp Ser Gly 725 730 735 Phe Leu Glu Tyr Arg His Thr Phe Lys Gln Asp Lys Leu Ala Asn Phe 740 745 750 Glu Arg Pro Thr Pro Ala His Asn Leu Val Asn Leu Gly Leu Asn Tyr 755 760 765 Gln His Lys Pro Ser His Gln Ala Gly Ser Val Gln Val Phe Phe Asn 770 775 780 Ala Asn Asn Leu Leu Asn Asp Lys Val Phe Ala His Glu Thr Phe Phe 785 790 795 800 Pro Asp Met Pro Gln Met Gly Arg Asn Phe Met Leu Gly Ala Asn Phe 805 810 815 Lys Phe 102766PRTMannheimia haemolytica 102Met Leu Lys Lys Asn Tyr Leu Thr Val Ser Ile Leu Leu Ala Ile Ser 1 5 10 15 Gly Val Gly Tyr Ala Asn Glu Ile Ser Leu Glu Thr Ile Thr Val Asp 20 25 30 Gly Asn Thr Pro Ser Thr Lys Gly Lys Leu Leu Gly Gly Glu Leu Asn 35 40 45 Ser Asn Glu Ser Val Val Asp Glu Lys Asn Leu Lys Gln Gly Ser Ile 50 55 60 Thr Leu Gly Asn Ala Leu Ser Gly Glu Leu Gly Ile His Ser Ser Gln 65 70 75 80 Phe Gly Gly Gly Ala Ser Thr Pro Ile Ile Arg Gly Gln Glu Ser Lys 85 90 95 Arg Ala Lys Ile Leu Gln Asn Asn Gly Glu Asn Leu Asp Met Ser Gly 100 105 110 Met Ser Pro Asp His Ala Val Thr Val Asp Ala Leu Leu Ala Lys Arg 115 120 125 Ile Glu Ile Leu Arg Gly Pro Thr Thr Leu Leu Tyr Ser Ala Gly Asn 130 135 140 Thr Ala Gly Val Ile Asn Val Val Asp Asn Lys Ile Pro Thr Ala Ile 145 150 155 160 Pro Glu Lys Gly Tyr Glu Gly Gln Phe Gly Val Arg Phe Gly Ser Ala 165 170 175 Ser Lys Glu Arg Leu Thr Tyr Ala Gly Ser Thr Phe Ala Leu Gly Asn 180 185 190 His Leu Ala Leu Arg Val Gln Gly Met Tyr Asn Lys Ala Ser Glu Tyr 195 200 205 Tyr Ala Pro His Phe Thr Ile Glu Gly Lys Pro Tyr His Arg Val Pro 210 215 220 Asp Ser Asp Val Gln Ser Gln Thr Gly Thr Val Ser Leu Ser Trp Ile 225 230 235 240 Gly Glu Arg Gly His Leu Gly Ile Ala Tyr Thr Asp Arg Arg Asp Lys 245 250 255 Tyr Gly Leu Ile Gly His Thr His Lys Tyr Asp His Tyr Thr Ile Ser 260 265 270 Ile Ile Arg Gln Ala Val Met Phe Ala Lys Gly Tyr Leu Arg Phe Tyr 275 280 285 Pro His Leu Ala Glu Glu Gly Asp Ile Asp Tyr Asn Asn Pro Gly Ile 290 295 300 Arg Leu Leu His Thr His Ile Pro Gly Gly Ser His Tyr Gly Gln Asp 305 310 315 320 Thr His Glu His Gly Lys Pro Trp Ile Asp Met His Ser Lys Arg Tyr 325 330 335 Asp Ile Asp Gly Ser Leu Gln Asn Pro Leu Pro Gly Phe Glu Glu Ala 340 345 350 Lys Ile Ser Ala Asn Tyr Val Asp Tyr Tyr His Asp Glu Lys Asp Gly 355 360 365 Lys Arg Val Glu Asn Tyr Phe Lys Asn Lys Gly Lys Asn Leu Arg Phe 370 375 380 Glu Leu Val His Lys Glu Trp Lys Gly Leu Lys Gly Ala Ile Gly Val 385 390 395 400 Gln Tyr Thr Asn Gln Ser Thr Ser Ala Leu Ala Leu Glu Ala Ser Arg 405 410 415 Ala Ala Lys Val Phe Asn Lys Gln Pro Leu Leu Asn Asn Pro Lys Thr 420 425 430 Lys Leu Trp Ser Leu Phe Ala Ile Glu Arg Leu Asn Leu Gly Asp Phe 435 440 445 Thr Phe Glu Leu Ser Gly Arg Ala Glu Arg Gln Lys Ile Ala Met Asp 450 455 460 Tyr Asp Val Lys Leu Ile Asp Arg Trp Leu Gly Phe Asn Thr Pro Met 465 470 475 480 Pro Asn Leu Asp Pro His Lys Asp Lys Gly Tyr Ser Tyr Ser Phe Ala 485 490 495 Thr His Trp Tyr Phe Ala Pro Asn His Lys Leu Thr Leu Asn Ala Ala 500 505 510 His Gln Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His Gly Lys 515 520 525 His Ile Ala Leu Asn Ala Phe Glu Ala Gly Asn Lys Asn Leu Lys Lys 530 535 540 Glu Arg Ser Asn Gln Ile Glu Leu Ser Leu Ala Tyr Val Gly Asp Lys 545 550 555 560 Trp Asp Tyr Lys Leu Asn Leu Tyr His Thr Arg Tyr Gly Asn Tyr Ile 565 570 575 Tyr Pro Leu Thr Leu Asn Asp Asn Arg Gly Pro Lys Ser Phe Thr Asp 580 585 590 Glu Tyr Asn Leu Lys Val Asn Arg Tyr Tyr Gln Gly Glu Ala Arg Phe 595 600 605 Ser Gly Ala Glu Gly Glu Ile Gly Tyr Leu Phe Thr Pro Asn Tyr Arg 610 615 620 Leu Ala Val Phe Gly Asp Tyr Val Arg Gly Lys Leu Val Asn Leu Pro 625 630 635 640 Asn Ile Ala Met Ser Tyr Asn Ile Trp Thr Gly Glu Val Asp Lys Trp 645 650 655 Ala Ser Gln Pro Asp Ile Ser Ala Pro Arg Ile Pro Pro Leu Arg Leu 660 665 670 Gly Ala Arg Phe Asn Ala Asp Phe Asn Leu Asn Trp Ser Gly Met Leu 675 680 685 Glu Tyr Tyr Arg Val Phe Ala Gln Lys Lys Val Ser Lys Tyr Glu Gln 690 695 700 Val Thr Pro Gly His His Gln Val Asn Leu Gly Val Thr Tyr Ser Asn 705 710 715 720 His Phe Asn Gln Thr Glu Tyr Gln Val Phe Leu Lys Val Asp Asn Leu 725 730 735 Leu Asn Gln Lys Met Tyr Gln His Ala Ser Tyr Leu Pro His Ile Pro 740 745 750 Gln Met Gly Arg Asn Ala Met Leu Gly Met Asn Ile Ser Phe 755 760 765 103797PRTPasteurella multocida 103Met Arg Lys Ile Ser Tyr Leu Ser Leu Cys Val Ile Ser Ala Leu Tyr 1 5 10 15 Ser Gln Leu Ala Val Ala Gln Ser Pro Leu Lys Asn Thr Ser Glu His 20 25 30 Ile Glu Leu Glu Pro Ile Phe Val Asn Thr Leu Ile Glu Ser Arg Glu 35 40 45 Gly Ala Pro Leu Gly Gly Arg Leu Met Ala Ser Glu Lys Ile Ile Pro 50 55 60 Ala Tyr Ser Leu Lys Gln Arg Gly Ser Asn Leu Gly Asp Ala Leu Ser 65 70 75 80 Ser Glu Leu Gly Ile His Ala Ser Gln Phe Gly Gly Gly Ala Ser Ala 85 90 95 Pro Val Ile Arg Gly Gln Glu Gly Lys Arg Ile Lys Val Leu Ser Ser 100 105 110 Gly Asn Glu Thr Leu Asp Met Ser Ala Met Ser Pro Asp His Ala Val 115 120 125 Ala Val Asp Ser Leu Leu Ala Lys Lys Val Glu Ile Leu Arg Gly Ala 130 135 140 Asn Thr Leu Leu Tyr Ser Ser Gly Asn Ala Ala Gly Val Val Asn Val 145 150 155 160 Val Asp Asn Lys Ile Pro Thr Ala Glu Val Val Gly Val Glu Gly Glu 165 170 175 Val Gly Leu Arg Thr Gly Ser Ala Asp Asn Glu Arg Leu Val Asn Val 180 185 190 Ala Leu Asp Val Gly Leu Ser Lys His Phe Ala Leu His Leu Glu Gly 195 200 205 Leu His Lys Lys Ala Gly Asp Tyr Arg Thr Pro Ser Tyr Gln Tyr Gln 210 215 220 Gly Ser Thr His His Lys Leu Ala Asn Ser Phe Val Asp Asn Arg Ser 225 230 235 240 Gly Ser Val Gly Leu Ser Trp Val Gly Asp Lys Gly Tyr Leu Gly Val 245 250 255 Ala Tyr Ser Gln Arg Lys Asp Lys Tyr Gly Leu Pro Ala His Ser His 260 265 270 Leu Tyr Asp Glu Tyr Tyr Met His Val Leu Leu Ser Asp Ala His Trp 275 280 285 Arg Lys Pro Tyr Leu Lys His Tyr Pro Phe Leu Met Glu Glu Thr Asp 290 295 300 Ile Asp Tyr Asn Asn Pro Gly Ile Asp Cys Ile Lys Lys Glu Trp His 305 310 315 320 Ser His Gly His Leu Cys Asn His Gly His Ala His His Gly Asn Gly 325 330 335 Gln His Ser His Asp His His Ala His Ala Asp Pro His Ile Ala Leu 340 345 350 Asn Thr Gln Arg Trp Asp Leu Arg Gly Glu Trp Lys Asn Pro Val Lys 355 360 365 Gly Leu Asp Lys Val Arg Phe Ser Ile Ala Lys Val Gly Tyr Arg His 370 375 380 Asp Glu Lys Ser Gly Ala Ile Ser Asp Asn Ser Phe Lys Asn Lys Gly 385 390 395 400 Tyr Ser Ala Arg Val Glu Phe Leu His Gln Pro Ile Ala Gly Val Ser 405 410 415 Gly Leu Ile Gly Leu Ser His Val Tyr Gln Asp Ser Tyr Ala Leu Asp 420 425 430 Asn His Thr Leu Glu Tyr Arg Lys Gln Asn Leu Leu Ser Asp His Thr 435 440 445 Thr Ala Gln Gln Ser Leu Phe Leu Met Glu His Val Glu Leu Gly Lys 450 455 460 Trp Gln Phe Asp Ile Gly Gly Arg Val Glu Lys Gln Arg Ile Ala Met 465 470 475 480 Lys Tyr His Phe Asn Val Pro Lys Asp Glu Gln Pro Pro Glu Glu Leu 485 490 495 Thr Arg Pro His Lys Ser Lys Ala Tyr Ser Tyr Ala Leu Ser Ala Asn 500 505 510 Tyr Gln Leu Asn Glu Gln His Gln Phe Asn Met Ile Val Ser His Gln 515 520 525 Glu Arg Leu Pro Asn Ala Gln Glu Leu Tyr Ala His Gly Lys His Leu 530 535 540 Ala Thr Asn Ser Phe Glu Ala Gly Asn Lys Asn Leu Thr Lys Glu Arg 545 550 555 560 Ser Asn Asn Val Glu Leu Gly Trp Gly Tyr Thr Gly Glu Lys Leu Gly 565 570 575 Ile Lys Leu Ser Gly Tyr Tyr Gln Gln Phe Ser Asn Tyr Ile Tyr Ala 580 585 590 Ala Ile Leu Asn Asn Lys Thr Ser Cys Pro Trp Arg Pro Asn Ser Arg 595 600 605 Cys Leu Arg Ser Leu Ser Asp Asp Tyr Pro Leu Arg Leu Tyr Arg Tyr 610 615 620 Asn Gln Ala Lys Ala Lys Ile Tyr Gly Leu Glu Ala Glu Val Ser Tyr 625 630 635 640 Gln Ile Ser Ser Thr His Ser Val Ser Ile Phe Gly Asp Tyr Val Arg 645 650 655 Gly Lys Leu Lys Asp Leu Pro Ser Leu Pro Ile Gly Tyr Lys Tyr Ile 660 665 670 Tyr Asn Glu Asn Tyr Asp Met Val Gly Val Gln Pro Thr Gly Trp Glu 675 680 685 Lys Gln Pro Asp Gly Asn Ala Pro Arg Met Ser Pro Met Arg Leu Gly 690 695 700 Ile Lys Trp Asn Ala Tyr Phe Asp Asn Gly Ile Ser Phe Asn Thr Gln 705 710 715 720 Leu Tyr Arg Val Phe Ala Gln Asn Lys Val Ala Arg Leu Glu Thr Pro 725 730 735 Thr Lys Gly His Thr Met Leu Asn Leu Gly Met Ser Tyr Asp Gly Lys 740 745 750 Met Gly Asn Asn Glu Tyr Thr Leu Phe Ala Asn Val Asn Asn Val Leu 755 760 765 Asn Ser Arg Val Tyr Asn His Thr Ser Phe Leu Ser Tyr Ile Pro Gln 770

775 780 Ser Gly Leu Gly Leu Asn Val Gly Met Asn Phe Lys Phe 785 790 795 104758PRTNeisseria meningitidis 104Met Ala Gln Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 15 Asn Thr Pro Leu Leu Ala Gln Ala His Glu Thr Glu Gln Ser Val Asp 20 25 30 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 35 40 45 Gly Leu Leu His Thr Ser Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 50 55 60 Thr Leu Arg Gln Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 65 70 75 80 Pro Gly Ile His Ala Ser Gln Tyr Gly Gly Gly Ala Ser Ala Pro Val 85 90 95 Ile Arg Gly Gln Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 Glu Thr Gly Asp Met Ala Asp Phe Ser Pro Asp His Ala Ile Met Val 115 120 125 Asp Thr Ala Leu Ser Gln Gln Val Glu Ile Leu Arg Gly Pro Val Thr 130 135 140 Leu Leu Tyr Ser Ser Gly Asn Val Ala Gly Leu Val Asp Val Ala Asp 145 150 155 160 Gly Lys Ile Pro Glu Lys Met Pro Glu Asn Gly Val Ser Gly Glu Leu 165 170 175 Gly Leu Arg Leu Ser Ser Gly Asn Leu Glu Lys Leu Thr Ser Gly Gly 180 185 190 Ile Asn Ile Gly Leu Gly Lys Asn Phe Val Leu His Thr Glu Gly Leu 195 200 205 Tyr Arg Lys Ser Gly Asp Tyr Ala Val Pro Arg Tyr Arg Asn Leu Lys 210 215 220 Arg Leu Pro Asp Ser His Ala Asp Ser Gln Thr Gly Ser Ile Gly Leu 225 230 235 240 Ser Trp Val Gly Glu Lys Gly Phe Ile Gly Val Ala Tyr Ser Asp Arg 245 250 255 Arg Asp Gln Tyr Gly Leu Pro Ala His Ser His Glu Tyr Asp Asp Cys 260 265 270 His Ala Asp Ile Ile Trp Gln Lys Ser Leu Ile Asn Lys Arg Tyr Leu 275 280 285 Gln Leu Tyr Pro His Leu Leu Thr Glu Glu Asp Ile Asp Tyr Asp Asn 290 295 300 Pro Gly Leu Ser Cys Gly Phe His Asp Asp Asp Asn Ala His Ala His 305 310 315 320 Thr His Ser Gly Arg Pro Trp Ile Asp Leu Arg Asn Lys Arg Tyr Glu 325 330 335 Leu Arg Ala Glu Trp Lys Gln Pro Phe Pro Gly Phe Glu Ala Leu Arg 340 345 350 Val His Leu Asn Arg Asn Asp Tyr Arg His Asp Glu Lys Ala Gly Asp 355 360 365 Ala Val Glu Asn Phe Phe Asn Asn Gln Thr Gln Asn Ala Arg Ile Glu 370 375 380 Leu Arg His Gln Pro Ile Gly Arg Leu Lys Gly Ser Trp Gly Val Gln 385 390 395 400 Tyr Leu Gln Gln Lys Ser Ser Ala Leu Ser Ala Ile Ser Glu Ala Val 405 410 415 Lys Gln Pro Met Leu Leu Asp Asn Lys Val Gln His Tyr Ser Phe Phe 420 425 430 Gly Val Glu Gln Ala Asn Trp Asp Asn Phe Thr Leu Glu Gly Gly Val 435 440 445 Arg Val Glu Lys Gln Lys Ala Ser Ile Gln Tyr Asp Lys Ala Leu Ile 450 455 460 Asp Arg Glu Asn Tyr Tyr Asn His Pro Leu Pro Asp Leu Gly Ala His 465 470 475 480 Arg Gln Thr Ala Arg Ser Phe Ala Leu Ser Gly Asn Trp Tyr Phe Thr 485 490 495 Pro Gln His Lys Leu Ser Leu Thr Ala Ser His Gln Glu Arg Leu Pro 500 505 510 Ser Thr Gln Glu Leu Tyr Ala His Gly Lys His Val Ala Thr Asn Thr 515 520 525 Phe Glu Val Gly Asn Lys His Leu Asn Lys Glu Arg Ser Asn Asn Ile 530 535 540 Glu Leu Ala Leu Gly Tyr Glu Gly Asp Arg Trp Gln Tyr Asn Leu Ala 545 550 555 560 Leu Tyr Arg Asn Arg Phe Gly Asn Tyr Ile Tyr Ala Gln Thr Leu Asn 565 570 575 Asp Gly Arg Gly Pro Lys Ser Ile Glu Asp Asp Ser Glu Met Lys Leu 580 585 590 Val Arg Tyr Asn Gln Ser Gly Ala Asp Phe Tyr Gly Ala Glu Gly Glu 595 600 605 Ile Tyr Phe Lys Pro Thr Pro Arg Tyr Arg Ile Gly Val Ser Gly Asp 610 615 620 Tyr Val Arg Gly Arg Leu Lys Asn Leu Pro Ser Leu Pro Gly Arg Glu 625 630 635 640 Asp Ala Tyr Gly Asn Arg Pro Phe Ile Ala Gln Asp Asp Gln Asn Ala 645 650 655 Pro Arg Val Pro Ala Ala Arg Leu Gly Phe His Leu Lys Ala Ser Leu 660 665 670 Thr Asp Arg Ile Asp Ala Asn Leu Asp Tyr Tyr Arg Val Phe Ala Gln 675 680 685 Asn Lys Leu Ala Arg Tyr Glu Thr Arg Thr Pro Gly His His Met Leu 690 695 700 Asn Leu Gly Ala Asn Tyr Arg Arg Asn Thr Arg Tyr Gly Glu Trp Asn 705 710 715 720 Trp Tyr Val Lys Ala Asp Asn Leu Leu Asn Gln Ser Val Tyr Ala His 725 730 735 Ser Ser Phe Leu Ser Asp Thr Pro Gln Met Gly Arg Ser Phe Thr Gly 740 745 750 Gly Val Asn Val Lys Phe 755 10514PRTArtificial Sequenceconserved motif 105Gly Xaa Xaa Xaa Pro Xaa Xaa Arg Gly Xaa Xaa Xaa Xaa Arg 1 5 10 10611PRTArtificial Sequenceconserved motif 106Asp Xaa Xaa Xaa Xaa Xaa Xaa Asp His Xaa Xaa 1 5 10 10711PRTArtificial Sequenceconserved motif 107Gly Xaa Xaa Xaa Xaa Xaa Asp Xaa Xaa Xaa Pro 1 5 10 10816PRTArtificial Sequenceconserved motif 108Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Gly Xaa Xaa Xaa Xaa 1 5 10 15 10930PRTArtificial Sequenceconserved motif 109Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa His Xaa Glu 20 25 30 11035PRTArtificial Sequenceconserved motif 110Arg Xaa Pro Xaa Xaa Xaa Glu Xaa Xaa Xaa Xaa Gly Xaa His Xaa Ala 1 5 10 15 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Xaa 20 25 30 Xaa Xaa Leu 35 11134PRTArtificial Sequenceconserved motif 111Tyr Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Asp Xaa Xaa 20 25 30 Xaa Xaa 1129PRTArtificial Sequenceconserved motif 112Xaa Arg Xaa Xaa Xaa Xaa Arg Xaa Xaa 1 5 11341PRTArtificial Sequenceconserved motif 113Arg Xaa Xaa Pro Gly Ala Xaa Thr Xaa Xaa Asp Xaa Xaa Xaa Gly Xaa 1 5 10 15 Xaa Ser Xaa Asn Xaa Arg Gly Xaa Xaa Gly Xaa Gly Arg Xaa Asn Xaa 20 25 30 Xaa Xaa Asp Gly Xaa Xaa Gln Thr Phe 35 40 11423PRTArtificial Sequenceconserved motif 114Phe Xaa Gly Xaa Xaa Gly Xaa Asn Xaa Leu Xaa Gly Ser Ala Asn Xaa 1 5 10 15 Xaa Thr Xaa Xaa Xaa Xaa Asp 20 11514PRTArtificial Sequenceconserved motif 115Ser Xaa Xaa Xaa Xaa Xaa Gln Xaa Xaa Xaa Xaa Gly Gly Gly 1 5 10 11635PRTArtificial Sequenceconserved motif 116Leu Xaa Lys Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Arg Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Arg Xaa Xaa Xaa Asn Xaa 20 25 30 Xaa Tyr Gln 35 11713PRTArtificial Sequenceconserved motif 117Pro Xaa Gly Xaa Gln Xaa Xaa Xaa Xaa Xaa Tyr Xaa Asp 1 5 10 11835PRTArtificial Sequenceconserved motif 118Asn Xaa Ser Xaa Xaa Xaa Ser Ala Xaa Xaa Xaa Xaa Xaa Phe Xaa Pro 1 5 10 15 Phe Xaa Xaa Xaa Xaa Xaa Xaa His Arg Xaa Pro Asn Xaa Xaa Glu Xaa 20 25 30 Xaa Phe Xaa 35 11915PRTArtificial Sequenceconserved motif 119Xaa Xaa Xaa Asn Xaa Xaa Asp Xaa Xaa Tyr Xaa Xaa Xaa Leu Xaa 1 5 10 15

Claims

1. An immunogenic composition comprising an antigen which is capable of raising an immune response to an outer membrane protein of at least a first Gram-negative bacterial strain, Wherein said protein is involved in uptake of extracellular zinc.

2. The immunogenic composition of claim 1, wherein the expression of said outer membrane protein is upregulated in response to a low extracellular zinc concentration.

3. The immunogenic composition of claim 1, wherein said first Gram-negative bacterial strain belongs to a bacterial genus or species selected from the group consisting of Acidovorax; Acinetobacter; Acinetobacter baumannii; Acinetobacter baumannii; Acinetobacter calcoaceticus; Acinetobacter johnsonii; Acmetohacter junii; Acinetobacter Iwoffii; Acinetobacter radioresistens; Actinobacillus; Actinobacillus minor; Actinobaciilus pleuropneurnoniae; Aggregatibacter; Aggregatibacter actinornycetemcomitans; Aggregatibacter aphrophilus; Alcanivorax; Alcanivorax borkumensis; Azoarcus; Azotobacter; Azotobacter vinelandii; Bordetella; Bordetella bronchiseptica; Bordetella parapertussis; Bordetella pertussis; Bordetella petrii; Campylobacter; Campylobacter coli; Campylobacter upsaliensis; Comamonas; Comamonas testosteroni; Delftia; Deiftia acidovorans; Diaphorobacter; Haemophilus; Haemophilus influenzae; Haemophilus parasuis; Haemophilus somnus; Mannheimia; Mannheimia haemolytica; Moraxella; Moraxella catarrhalis; Neisseria; Neisseria gonorrhoeae; Neisseria meningitidis; Pasteurella; Pasteurella dagmatis; Pasteurella multocida; Proteus Proteus mirabilis; Pseudomonas; Pseudomonas stutzeri; and Sphingornonas.

4. The immunogenic composition of claim 1, comprising an antigen comprising a polypeptide having at least 95% identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO 6; SEQ ID NO: 7; SEQ ID NO: 8; SEQ ID NO: 9; SEQ ID NO: 10; SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO: 16; SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; SEQ ID NO: 24; SEQ ID NO: 25; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 22; SEQ ID NO: 29; SEQ ID NO: 30; SEQ ID NO: 31; SEQ ID NO: 32; SEQ II) NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37, SEQ. ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40 SEQ ID NO: 41; SEQ ID NO: 42; SEQ NO: 43, SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; SEQ ID NO: 51; SEQ ID NO: 52; SEQ ID NO: 53; SEQ ID NO: 54; SEQ ID NO: 55; SEQ ID NO: 56; SEQ ID NO: 57; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SRI ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; SEQ ID NO: 65; SEQ ID NO: 66; SEQ ID NO: 67; SEQ ID NO: 62; SEQ ID NO: 69; SEQ ID NO: 70; and SEQ ID NO: 71.

5. The immunogenic composition of claim 1, comprising an antigen comprising a polypeptide comprising an immunogenic fragment of a sequence selected from the group consisting of SEQ ID NO: 1; SEQ ID NO: 2; SEQ ID NO: 3; SEQ ID NO: 4; SEQ ID NO: 5; SEQ ID NO: 6; SEQ ID NO: 7; SEQ ID NO: 8; SEQ It) NO: 9; SEQ ID NO: 10; SEQ ID NO: 11; SEQ ID NO: 12; SEQ ID NO: 13; SEQ ID NO: 14; SEQ ID NO: 15; SEQ ID NO: 16; SEQ ID NO: 17; SEQ ID NO: 18; SEQ ID NO: 19; SEQ ID NO: 20; SEQ ID NO: 21; SEQ ID NO: 22; SEQ ID NO: 23; SEQ ID NO: 24; SEQ ID NO: 25; SEQ ID NO: 26; SEQ ID NO: 27; SEQ ID NO: 28; SEQ ID NO:29; SEQ ID NO: 30; SEQ ID NO: 31; SEQ ID NO: 32; SEQ ID NO: 33; SEQ ID NO: 34; SEQ ID NO: 35; SEQ ID NO: 36; SEQ ID NO: 37; SEQ ID NO: 38; SEQ ID NO: 39; SEQ ID NO: 40; SEQ ID NO: 41; SEQ ID NO: 42; SEQ ID NO: 43; SEQ ID NO: 44; SEQ ID NO: 45; SEQ ID NO: 46; SEQ ID NO: 47; SEQ ID NO: 48; SEQ ID NO: 49; SEQ ID NO: 50; SEQ ID NO: 51; SEQ ID NO: 52; SEQ ID NO: 53; SEQ ID NO: 54; SEQ ID NO: 55; SEQ ID NO: 56; SEQ ID NO: 57; SEQ ID NO: 58; SEQ ID NO: 59; SEQ ID NO: 60; SEQ ID NO: 61; SEQ ID NO: 62; SEQ ID NO: 63; SEQ ID NO: 64; SEQ ID NO: 65; SEQ ID NO: 66; SEQ ID NO: 67; SEQ ID NO: 68; SEQ ID NO: 69; SEQ ID NO: 70; and SEQ ID NO: 71, wherein said immunogenic fragment consists of an amino acid sequence having 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 40, 50 or more contiguous amino acids of said selected sequence.

6. The immunogenic composition of claim 1, wherein said immunogenic composition does not comprise a polypeptide having the amino acid sequence of an NMB0964 polypeptide according to PCT/EP2009/052689 or a fragment thereof as disclosed therein, SEQ ID NO: 2 of WO 00/55327 or a fragment thereof as disclosed therein, SEQ ID NO; 606 of WO 99/57280 or a fragment thereof as disclosed therein, or SEQ ID NOs. 2, 4 and 6 of WO 00/11182 or a fragment thereof as disclosed therein.

7. The immunogenic composition of claim 1 comprising at least one additional antigen selected from the group consisting of a fHbp polypeptide, a Hsf polypeptide, a Hap polypeptide, a NadA polypeptide and a lipo28 polypeptide, or an immunogenic fragment thereof.

8. The immunogenic composition of claim 1, comprising a bacterial outer membrane vesicle preparation comprising said antigen, wherein expression of said antigen is up-regulated in said outer membrane vesicle preparation.

9. The immunogenic composition of claim 1, further comprising a zinc salt.

10. An immunogenic composition comprising: (a) an antigen, which antigen comprises a polypeptide having at least 95% identity to the amino acid sequence of SEQ ID NO: 50, or (b) an antigen, which antigen comprises a polypeptide comprising an immunogenic fragment of the sequence of SEQ ID NO: 50, wherein said immunogenic fragment consists of an amino acid sequence having 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 30, 40, 50 or more contiguous amino acids of the sequence of SEQ ID NO: 50, wherein said immunogenic fragment is capable of raising an immune response which recognises the amino acid sequence of SEQ ID NO: 50.

11. The immunogenic composition of claim 10, further comprising an antigen comprising a polypeptide having at least 95% to the amino add, sequence of SEQ ID NO: 1, further comprising an antigen comprising a polypeptide comprising an immunogenic fragment of the sequence of SEQ ID NO: 1, wherein said immunogenic fragment consists of an amino acid sequence having 7, 8, 9, 10, 11, 12, 13, 14, 15, 20, 30, 40, 50 or more contiguous amino acids of the sequence of SEQ ID NO: 1, and wherein said immunogenic fragment is capable of raising, an immune response which recognises the amino acid sequence of SEQ ID NO: 1.

12. A method for producing an immunogenic composition of claim 10 comprising: culturing a host cell comprising an expression vector comprising a polynucleotide encoding said antigen under conditions and for a time sufficient for the production of said antigen and recovering the antigen from the culture medium; optionally purifying said antigen by a method selected from the group consisting of ammonium sulphate precipitation, ethanol precipitation, acid extraction, phosphocellulose chromatography, hydrophobic interaction chromatography, hydroxylapatite chromatography and lectin chromatography; and formulating said antigen with a pharmaceutically acceptable carrier or excipient.

13. A method for producing an immunogenic composition of claim 10, comprising culturing at least a first Gram-negative bacterial strain which produces said antigen, wherein said antigen is produced at, a level sufficient to provide for production of outer membrane vesicles that, when administered to a subject, raise a protective response against infection, or disease caused, by at least said first Gram-negative bacterial strain; preparing outer membrane vesicles from the cultured strain; and combining said outer membrane vesicles with a pharmaceutically acceptable carrier or excipient to produce an immunogenic composition suitable for administration to a subject.

14. A method of preparing an immune globulin for prevention or treatment of Gram-negative bacterial disease, comprising the steps of: (a) immunising a recipient with An immunogenic composition comprising an antigen which is capable of raising an immune response to an outer membrane protein of at least a first Gram-negative bacterial strain, wherein said protein is involved in uptake of extracellular zinc; and (b) isolating immune globulin from the recipient.

15. The immunogenic preparation obtained from the method of claim 13.

16. A method for the treatment or prevention of Gram-negative bacterial, for instance Neisserial, infection or disease comprising administering a protective dose or an effective amount of the immunogenic composition of claim 1.

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