METHODS AND COMPOSITIONS FOR THE DETECTION OF TARGET MOLECULES

Abstract

The present disclosure provides methods and compositions for the detection of target molecules, comprising modified bacteriophages engineered to express a luciferase.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of U.S. Provisional Application No. 61/643,534, filed May 7, 2012, the entire contents of which are hereby incorporated by reference.

FIELD OF TECHNOLOGY

[0002] This technology relates generally to the field of biotechnology, and in particular to the field of molecular detection.

BACKGROUND

[0003] Enzyme-linked immunosorbent assay (ELISA) has been used as a versatile and powerful tool both in basic science and clinical diagnostics since the mid-1960s. This assay makes use of the physical connection established between an antigen-recognizing moiety and device capable of generating a visual signal. The specificity of the assay is mostly determined by the first component while the sensitivity is determined by the second. Traditionally, as the antigen-recognizing moiety in ELISA, monoclonal or polyclonal antibodies are used and enzymes such as alkaline phosphatase or horse radish peroxidase serve as the device generating the visual signal.

[0004] Often, conventional ELISA is not sensitive enough and a wide range of labeling and signal-enhancement strategies have been developed in an attempt to increase its sensitivity. A so-called DNA-enhanced immunoassay strategy stands out as the most sensitive one. This strategy employs antigen-recognizing molecules carrying DNA tags and relies on the ability of DNA polymerases to exponentially amplify these tags. Because of this amplification, the sensitivity of the assay becomes 10-10,000-fold higher than the sensitivity of traditional antigen detection methods. Originally, this strategy was described by Sano et al. in 1992 and was called immuno-PCR (IPCR). Later, a number of modifications of this strategy were developed that targeted two major areas: 1) coupling of immunoassay reagents and DNA markers, and 2) assay readout.

[0005] While the original Sano's IPCR was modular and utilized a linker protein that allowed the binding of the Fc part of an IgG and subsequent tagging with biotinylated dsDNA, it has been demonstrated that the performance of pre-assembled antibody-DNA conjugates exceeds that of the stepwise assembled complexes in the modular approach. Several approaches have been proposed that allow direct coupling of DNA tags with antigen-recognizing molecules. For example, Guo et al. described a phage display-mediated IPCR assay that makes use of filamentous bacteriophage M13 to expose on its surface single chain variable fragments (scFv) of IgG antibodies. In this assay, entire phage particles were used for binding to the target antigen and simultaneously their DNA served as a tag that was amplified by PCR upon completion of the antigen-binding portion of the reaction.

[0006] Among approaches that try to improve assay readout, the approach termed Expression Immunoassay deserves special attention. It takes advantage of a biotinylated DNA tag which encodes the firefly luciferase. In this approach, formation of the immuno-complex is followed by a one-step, cell-free translation expression step, which enables the detection of 3000 DNA molecules or 50 000 antigen molecules, respectively.

[0007] While being substantially more sensitive than conventional ELISA, DNA-enhanced immunoassays turn out to be significantly more difficult to perform and more expensive than conventional ELISA.

SUMMARY

[0008] In one aspect, the present disclosure provides a method for detecting a target molecule, comprising: contacting an immobilized target molecule with a modified bacteriophage encoding a luciferase, wherein the bacteriophage specifically binds the target molecule, under conditions that promote binding of the bacteriophage to the target molecule, to produce an target molecule-bacteriophage complex; contacting the target molecule-bacteriophage complex with a bacterial strain susceptible to infection by the bacteriophage; incubating the target molecule-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the luciferase, to produce a bacteriophage-infected culture; adding to the bacteriophage-infected culture a substrate for the luciferase; and measuring the presence and/or magnitude of fluorescence produced from the action of the luciferase on its substrate.

[0009] In some embodiments, the target molecule comprises a first affinity tag and the bacteriophage comprises a second affinity tag, wherein the first and second affinity tags specifically bind. In some embodiments, the first and second affinity tags are enzymatically or chemically coupled to the target molecule and the bacteriophage. In some embodiments, the first affinity tag is streptavidin and the second affinity tag is biotin. In some embodiments, the bacteriophage is engineered to bind directly to the target molecule using phage display.

[0010] In some embodiments, the modified bacteriophage is derived from an M13, T, T7, or .lamda. bacteriophage. In some embodiments, the modified bacteriophage comprises SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, or SEQ ID NO:4.

[0011] In another aspect, the present disclosure provides a method for detecting a target molecule, comprising: contacting an immobilized target molecule with a first affinity ligand that specifically binds the target molecule, under conditions that promote target molecule-affinity ligand binding, to form a target molecule-first affinity ligand complex, and optionally contacting the target molecule-first affinity ligand complex with one or more additional antibodies under conditions that promote affinity ligand-affinity ligand binding, to produce a target molecule-first affinity ligand-additional affinity ligand complex, wherein the one or more antibodies are added sequentially, and wherein each successive affinity ligand specifically binds to the affinity ligand added immediately previous; contacting the target molecule-first affinity ligand complex or the target molecule-first affinity ligand-additional affinity ligand complex with a modified bacteriophage encoding a luciferase, wherein the bacteriophage binds to the first affinity ligand, or if one or more additional antibodies are used, binds to the ultimate affinity ligand, to form a target molecule-affinity ligand-bacteriophage complex; contacting the target molecule-affinity ligand-bacteriophage complex with a bacterial strain susceptible to infection by the bacteriophage; incubating the target molecule-affinity ligand-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the luciferase, to produce a bacteriophage-infected culture; adding to the bacteriophage-infected culture a substrate for the luciferase; and measuring the presence and/or magnitude of fluorescence produced from the action the luciferase on its substrate.

[0012] In some embodiments, the first affinity ligand, or if one or more additional antibodies are used, the ultimate affinity ligand, comprises a first affinity tag, and the bacteriophage comprises a second affinity tag, wherein the first and second affinity tags specifically bind. In some embodiments, the first and second affinity tags are enzymatically or chemically coupled to the affinity ligand and the bacteriophage. In some embodiments, the first affinity tag is streptavidin and the second affinity tag is biotin. In some embodiments, the bacteriophage is engineered to bind directly to the affinity ligand using phage display.

[0013] In some embodiments, the modified bacteriophage is derived from an M13, T, T7, or .lamda. bacteriophage. In some embodiments, the modified bacteriophage comprises SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, or SEQ ID NO:4.

[0014] In one aspect, the present disclosure provides a nucleic acid comprising a modified bacteriophage encoding a luciferase. In some embodiments, the modified bacteriophage is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4. In some embodiments, the nucleic acid comprises an affinity tag. In some embodiments, the affinity tag is enzymatically or chemically coupled to the bacteriophage. In some embodiments, the bacteriophage is engineered to bind directly to an affinity ligand or to a target molecule using phage display.

BRIEF DESCRIPTION OF THE FIGURES

[0015] FIGS. 1A and 1B show the detection of luciferase-encoding derivatives of phages T7 and .lamda.. Different amounts of phage particles were incubated with 100 .mu.l of overnight culture of the appropriate host diluted in LB. Upon completion of incubation, 50 .mu.l of 0.004% of decanal in water was added to each well containing pSL-LuxAB and measurements of luminescence were performed on Veritas luminometer. In the case of the T7 derivative, decanal was present in the mixture during incubation time and measurements were taken automatically. Bacteriophage cultures were incubated for 10, 30, 45, 65, or 70 minutes prior to fluorescence measurement.

[0016] FIGS. 2A and 2B show the accumulation of luciferase in cells infected with M13LuxABE2. Dilutions of overnight culture were infected with different amounts of M12LuxABE2 and allowed to grow in LB media supplemented with IPTG for 4 hours at 37.degree. C. with aeration prior to measuring luciferase activity. The luciferase activity of 50 .mu.l aliquots was determined after addition of 25 .mu.l of 0.004% decanal on Veritas luminometer. 450 .mu.l aliquots of bacterial cultures diluted 100 times with LB supplemented with IPTG were infected with 10.sup.6 particles of M13LuxABE2 and were incubated for 3 hours at 37.degree. C. without aeration prior to measuring luciferase activity. Then, the luciferase activity was determined as described herein.

[0017] FIG. 3 shows the detection of rabbit serum with T71-2aLuxAB-spAG conjugate. Subsequent dilutions of rabbit serum were attached to wells of a 96-well plate and the remaining centers for nonspecific sorption were blocked with 1% BSA dissolved in PBS with 0.1% Tween 20 (PBST). Then, wells were filled with PBST containing diluted T71-2aLuxAB-spAG conjugate and the plate was incubated for 1 hour at 37.degree. C. After completion of incubation, wells were washed ten times with PBST and filled with 100 .mu.l of overnight E. coli BLT5615 culture diluted 1:50 in LB. They were subsequently incubated for 1 hour at 37.degree. C. without aeration. Then, 50 .mu.l of 0.004% of decanal in water was added to each well and measurements of luminescence were performed on Veritas luminometer. The bacteriophage culture was incubated for 60 minutes prior to fluorescence measurement.

[0018] FIGS. 4A and 4B show the detection of SpAG and streptavidin with luciferase-encoding phages. Different quantities of immobilized SpAG were incubated with M13LuxABESspAGVII2 for one hour and then the immobilized phage was detected via addition of overnight culture ER2738 diluted 1:100 with LB supplemented with IPTG to wells. Incubation was conducted for 4 hours at 37.degree. C. without aeration. Different quantities of immobilized streptavidin were incubated with M13LuxABE-SstrepVII25-1 for either 16 hours at room temperature or for 1 hours at 37.degree. C. After completion of incubation and removal of unbound phage, 50 .mu.l of either PBS alone or PBS containing 5 mM of biotin were added to each set of wells and plates were incubated for 30 min at 37.degree. C. prior to addition of 1:100 diluted overnight culture of ER2738. After 4 hours of incubation at 37.degree. C. without aeration, luciferase activity was determined as described in FIG. 1.

[0019] FIG. 5 shows the detection of streptavidin by PLISA and ELISA. Wells with immobilized streptavidin were incubated with either non-biotinylated or biotinylated M13LuxABElink-SompbioVII3 or biotinylated horse radish peroxidase for 1 hour at 37.degree. C. Then, the unbound material was washed away and the immobilized phage was detected as described in FIG. 2 while the horse radish peroxidase was detected by addition of 1-Step.TM. Slow TMB-ELISA (Thermo Scientific) into wells.

[0020] FIGS. 6A and 6B show the detection of fragments of botulinum neurotoxin by PLISA and ELISA. Wells covered with either BoNT/A-L (Panel A) or BoNT/A-CH (Panel B) were subsequently blocked with normal goat serum, incubated with rabbit antibodies raised to botulinum neurotoxin A, incubated with horse biotinylated anti-rabbit antibodies, peroxidase, and either biotinylated M13LuxABElink-SompbioVII3 or biotinylated horse radish peroxidase. Except for BoNT/A fragments, anti-BoNT and phage, all other components come from VectaStain ABC kit (Vector Laboratories Inc., CA). Detection of phage and horse radish peroxidase was done as described in the legend of FIG. 3.

DETAILED DESCRIPTION

[0021] The present disclosure provides compositions and methods for the detection of molecules. In some embodiments, the methods comprise the use of bacteriophages engineered to express a bacterial luciferase and to comprise an affinity tag or other label. In some embodiments, the bacteriophage is chemically conjugated with an affinity tag or other label. In some embodiments, expression of a bacteriophage-encoded bacterial luciferase is used to detect the presence of a molecule of interest.

[0022] To create an assay that offers high sensitivity in combination with simplicity of performance and low cost, here, we introduced a new type of engineered bacteriophage that exposes antigen-recognizing moieties on the surface of its particles and simultaneously encodes bacterial luciferase. Earlier, it was demonstrated that, in addition to proteins III and V that are traditionally used for exposing foreign proteins on the surface of capsids of filamentous E. coli phage M13, protein VII can also be used for such a role. Unlike protein III, protein VII is not involved in the recognition of bacterial receptors. Here, we demonstrated that phages exposing antigen-recognizing polypeptides as part of protein VII can be attached to immobilized antigens and retain their ability to infect host bacterial cells. Also, we demonstrated that phages exposing antigen-recognizing moieties on the surface of their particles can be generated via chemical conjugation of these particles with corresponding antigen-recognizing moieties.

[0023] It has also been determined that several marine (Vibrio harveyi and Vibrio fischeri) and terrestrial (Photorhabdus luminescence) species of bacteria produce homologous luciferases that catalyze the oxidation of long-chain aldehydes and produce photons as one of the reaction products. In all species, sequences encoding subunits of these enzymes (luxA and luxB) are expressed as part of a larger operon that also encodes proteins responsible for synthesis of long-chain aldehydes. The luxA and luxB genes in all of these operons, however, are located next to each other, allowing their separation from the rest of the operon and isolation on a relatively short DNA fragment. Luciferase produced by P. luminescence has substantially higher thermostability than homologous enzymes from V. harveyi and V. fischeri. Also, it has a higher thermostability than firefly luciferase, which was used in the herein-described Expression Immunoassay.

[0024] It is to be understood that the present methods may be practiced using any luciferase enzyme, whether derived from a bacterial or a non-bacterial source. However, luciferase from P. luminescence presents certain advantages for expression in E. coli as compared to non-bacterial luciferases. For example, the substrate for P. luminescence luciferase is substantially less expensive than the substrate for firefly luciferase and, unlike the latter, is capable of penetrating through the cell membrane. This eliminates the need for lysis of cells that is required when activity of firefly luciferase in cells has to be measured. Thus, bacterial luciferase may be selected for the current methods, depending on operator preference.

[0025] By introducing P. luminescence genes for luciferase into genomes of phages and exposing antigen-recognizing polypeptides on the surface of phage particles (as part of capsid protein VII or via chemical conjugation of such polypeptides with phage particles) we created new tools for detection of molecules of interest. These tools can be used in a phage-linked immunosorbent assay (PLISA), which is similar to the conventional ELISA. While being less expensive than reagents used in conventional ELISA, these tools allow the sensitivity of detection to exceed the sensitivity of conventional ELISA by 10-1000 times and makes it comparable to that of IPCR.

[0026] As used herein, "bacteriophage" means a virus that infects and replicates within bacteria, composed of proteins that encapsulate a DNA or RNA genome. In some embodiments, the bacteriophage comprises an M13 bacteriophage. In some embodiments, the bacteriophage comprises a T or T7 bacteriophage. In some embodiments, the bacteriophage comprises a .lamda. bacteriophage. In some embodiments, the bacteriophage comprises SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, or SEQ ID NO: 4. In some embodiments, the bacteriophage is engineered to encode an affinity tag or other label. In some embodiments, the bacteriophage is enzymatically or chemically labeled with an affinity tag or other label. In some embodiments, the bacteriophage is engineered to express an affinity tag or other label using phage display.

[0027] As used herein, "affinity tag" means a label that is appended to a protein or other molecule for purposes of purification or detection. Non-limiting examples of affinity tags commonly used in the art include chitin binding protein (CBP), maltose binding protein (MBP), and glutathione-S-transferase (GST), poly-histidine (HIS), streptavidin, and biotin. In some embodiments, the affinity tag is streptavidin. In some embodiments, the affinity tag is biotin. In some embodiments, the affinity tag is a hybrid of staphylococcal protein A and streptococcal protein. As used herein "affinity ligand" means any molecule that binds with high affinity to a target molecule of interest. In some embodiments, the affinity ligand is an antibody.

[0028] In some embodiments, the target molecule of interest comprises a first affinity tag, and the bacteriophage comprises a second affinity tag, wherein the first and second affinity tags interact. In some embodiments, the bacteriophage is engineered bind directly to a target molecule or an affinity ligand, in the absence of an affinity tag on the bacteriophage, target molecule, or affinity ligand. In some embodiments, the affinity ligand is an antibody.

[0029] In some embodiments, a target molecule of interest and an engineered bacteriophage bind via the use of affinity tags. In some embodiments, a target molecule of interest and an engineered bacteriophage bind via the use of other labels, such as a compatible binding pairs, including, but not limited to, antibody-antigen pairs, complementary nucleic acids, enzyme-substrate pairs, aptamer-protein pairs, nucleic acid-protein pairs, hormone-ligand pairs, and receptor-ligand pairs. One of skill in the art will understand that the methods described herein may be practiced using any means to bind the target molecule of interest to the engineered bacteriophage.

[0030] In some embodiments, the target molecule of interest and the bacteriophage bind directly, such as through the biding of affinity tags present on the target molecule and the bacteriophage. In some embodiments, the target molecule of interest and the bacteriophage bind indirectly, such as through the use of one or more antibody intermediates. In some embodiments, an target molecule of interest is contacted by an antibody comprising a first affinity tag, which is in turn contacted by a bacteriophage comprising a second affinity tag that binds to the first affinity tag. In some embodiments, the target molecule of interest is contacted by an antibody, which is in turn contacted by an additional antibody, comprising the second affinity tag. In some embodiments, the target molecule of interest is contacted by an antibody, followed by a series of antibodies added sequentially, wherein each successive antibody specifically binds to the antibody added previously, and wherein the final antibody comprises a first affinity tag that binds to a second affinity tag present on a bacteriophage. In some embodiments, the bacteriophage is engineered to bind directly to a target molecule or an antibody.

[0031] As used herein, "bacterial luciferase" refers to the luciferase enzyme encoded by one of various bacterial species. In some embodiments, the bacterial species are of the genus Photorhabdus. In some embodiments, the bacterial species are of the genus Vibrio. In some embodiments, the bacterial species are of the genus Photobacterium. In some embodiments, the bacterial luciferase is that of Photorhabdus luminescence. In some embodiments, the bacterial luciferase is that of Vibrio harveyi. In some embodiments, the bacterial luciferase is that of Vibrio fischeri. In some embodiments, the bacterial luciferase comprises all the subunits of the bacterial lux operon. In some embodiments, the bacterial luciferase comprises a subset of the subunits of the bacterial lux operon. In some embodiments, methods described herein comprise the use of a bacterial luciferase substrate. In some embodiments, the bacterial luciferase substrate is decanal

[0032] As used herein, "modified bacteriophage" refers to a bacteriophage that has been modified to express a detectable marker. In some embodiments, the detectable marker is a fluorescent protein. In some embodiments the detectable marker is green fluorescent protein (GFP) or a variant of GFP. In some embodiments the detectable marker is an enzyme. In some embodiments, the detectable marker is a luciferase. In some embodiments, the modified bacteriophage has been further engineered to comprise an affinity tag. In some embodiments, the modified bacteriophage has been further engineered to specifically bind to a target molecule or an antibody or other affinity ligand, such as, for example, using phage display. In some embodiments, the luciferase is a bacterial luciferase. In some embodiments, the luciferase is derived from non-bacterial sources, such as, for example, firefly, click beetle, sea pansy, dinoflagellates, or copepod.

[0033] As used herein "target molecule" refers to a molecule of interest, i.e., a molecule to be detected or quantified. The methods and compositions disclosed herein are not intended to be limited by the type of target molecule. The methods may be used, for example, for the detection of any kind of polypeptide, polysaccharide, lipid, or nucleic acid for which interacting molecules are available which could be directly or indirectly attached to bacteriophage particles. In some embodiments, the target molecule is a protein. In some embodiments, the target molecule is a polypeptide. In some embodiments, the target molecule is a native polypeptide. In some embodiments, the target molecule is a modified polypeptide. In some embodiments, the target molecule is a polysaccharide. In some embodiments, the target molecule is a lipid. In some embodiments, the target molecule is a nucleic acid. In some embodiments the target molecule is a chimeric protein. In some embodiments, the target molecule is an antigen.

Methods

[0034] In one aspect, the present disclosure provides methods for the detection of molecules. In some embodiments, the methods comprise the use of a bacteriophage engineered to express a bacterial luciferase and to comprise an affinity tag or other label.

[0035] In some embodiments, the bacteriophage comprises an M13, T, T7, or .lamda. bacteriophage. In some embodiments, the bacteriophage encodes a Photorhabdus luminescence luciferase. In some embodiments, the bacterial luciferase comprises all the subunits of the bacterial lux operon. In some embodiments, the bacterial luciferase comprises a subset of the subunits of the bacterial lux operon. In some embodiments, the bacteriophage encodes one or more of the A, B, and E subunits of the lux operon. In some embodiments, the bacteriophage encodes one or more of the A, B, and E subunits of the lux operon.

[0036] In some embodiments, the bacteriophage is enzymatically or chemically coupled to an affinity tag or other label. In some embodiments, the affinity tag is biotin. In some embodiments, the affinity tag is encoded by the bacteriophage. In some embodiments, the bacteriophage is engineered to express a protein sequence (i.e. an affinity tag) that specifically binds to a specific protein using phage display. In some embodiments, the bacteriophage is engineered to display a protein that specifically binds to a particular affinity tag or label of interest. In some embodiments, the bacteriophage comprise SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4.

[0037] In some embodiments, the method comprises a) contacting an immobilized molecule with a modified bacteriophage encoding a bacterial luciferase, wherein the molecule comprises a first affinity tag and the bacteriophage comprises a second affinity tag, and wherein the first and second affinity tags specifically bind, under conditions that promote binding of the first and second affinity tags, to produce an molecule-bacteriophage complex; b) contacting the molecule-bacteriophage complex with a bacterial strain susceptible to infection by the bacteriophage; c) incubating the molecule-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the bacterial luciferase, to produce a bacteriophage culture; d) adding to the bacteriophage culture a substrate for the bacterial luciferase; and e) measuring the presence and/or magnitude of fluorescence produced from the action of the bacterial luciferase on its substrate.

[0038] In some embodiments, the method comprises a) contacting an immobilized molecule with a first antibody that specifically binds the molecule, under conditions that promote molecule-antibody binding, to form a molecule-first antibody complex, and optionally contacting the molecule-first antibody complex with one or more additional antibodies under conditions that promote antibody-antibody binding, to produce a molecule-first antibody-additional antibody complex, wherein the one or more antibodies are added sequentially, and wherein each successive antibody specifically binds to the antibody added immediately previous, and wherein either the first antibody comprises a first affinity tag, or if one or more additional antibodies are added, the ultimate antibody comprises a first affinity tag; b) contacting the molecule-first antibody complex or the molecule-first antibody-additional antibody complex of a) with a modified bacteriophage encoding a bacterial luciferase and comprising a second affinity tag that specifically binds to the first affinity tag, under conditions that promote binding of the first and second affinity tags, to form a molecule-antibody-bacteriophage complex; c) contacting the molecule-antibody-bacteriophage complex of b) with a bacterial strain susceptible to infection by the bacteriophage; d) incubating the molecule-antibody-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the bacterial luciferase, to produce a bacteriophage culture; e) adding to the bacteriophage culture of d) a substrate for the bacterial luciferase; and f) measuring the presence and/or magnitude of fluorescence produced from the action the bacterial luciferase on its substrate.

Compositions

[0039] In one aspect, the present disclosure provides compositions for the detection of molecules.

[0040] In some embodiments, the compositions comprise one or more nucleic acids comprising a bacteriophage engineered to express a bacterial luciferase. In some embodiments, the modified bacteriophage is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4. In some embodiments, the nucleic acid comprises an affinity tag. In some embodiments, the affinity tag is encoded by the bacteriophage or is enzymatically or chemically coupled to the bacteriophage. In some embodiments, the bacteriophage is engineered to express the second affinity tag by phage display.

Kits

[0041] In one aspect, the present disclosure provides kits for the detection of molecules.

[0042] In some embodiments, the kits comprise one or more nucleic acids comprising a bacteriophage engineered to express a bacterial luciferase. In some embodiments, the modified bacteriophage is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO:4. In some embodiments, the nucleic acid comprises an affinity tag. In some embodiments, the affinity tag is encoded by the bacteriophage or is enzymatically or chemically coupled to the bacteriophage. In some embodiments, the bacteriophage is engineered to express the second affinity tag by phage display.

[0043] In some embodiments, the kits comprise one or more positive and/or negative control samples, and instructions for use.

Examples

[0044] This example demonstrates the compositions and methods comprising the use of engineered bacteriophages for the detection of molecules.

Materials and Methods

[0045] Cells--

[0046] The Escherichia coli DH5.alpha.F strain and its derivative AU-DH10, carrying F'-factor with tetracycline resistance marker, were used for routine propagation of plasmids and derivatives of phage M13. Strains BLT5615 and LE392 were used propagate derivatives of phages T7 and .lamda., respectively. Strain BL21(DE3) was used for expression of plasmids encoding recombinant proteins. Strain AU-SE2 was used for generation of MD12.TM. phage display. This strain was constructed on the basis of strain MC1061 via conjugation with strain ER2738 followed by P1-mediated transduction of mutS201::Tn5(KmR) from ES1301 mutS. Strain ER2738 was used for propagation of luciferase-encoding phages in PLISA.

[0047] Production of Recombinant Proteins--

[0048] BL21(DE3) cells carrying plasmids for streptavidin were grown in LB media at 37.degree. C. with aeration until culture reached OD.sub.600 .about.0.4 and expression was induced via addition of IPTG. After induction, cultures were kept aerated at 37.degree. C. for 3 hours and then cells were harvested by centrifugation, resuspended in PBS and stored at -84.degree. C. until further use. BL21(DE3) cells carrying plasmids for E. coli biotin ligase (BirA), a hybrid between staphylococcal protein A and streptococcal protein G (SpAG) or the receptor-recognizing domain of botulinum neurotoxin serotype A (BoNT/A-CH5) were treated similarly, except for that they were grown until OD.sub.600 .about.0.6 before induction and they were grown overnight at 16.degree. C. after induction. Plasmids encoding all of these proteins were constructed in our lab earlier and possess Ni-affinity tag incorporated in their structure. Streptavidin that accumulated inside cells in the form of inclusion bodies was purified via denaturation in 6 M guanidine followed by renaturation in PBS. BirA, SpAG and BoNT/A-CH5 were accumulated inside cells in the soluble form and were purified via affinity chromatography on Ni-NTA Superflow (Qiagen). The amino acid sequence of the SpAG polypeptide is given in Table 2. The amino acid sequence of the BoNT/A-CH5 polypeptide is given in Table 3.

[0049] DNA Modifying Enzymes--

[0050] All restriction endonucleases, as well as T4 DNA-polymerase, Rapid DNA Ligation Kit and Expand.TM. High Fidelity PCR System were supplied by Fermentas.

[0051] Oligonucleotides--

[0052] Oligonucleotides used for polymerase chain reaction, as well as oligonucleotides used for cloning are listed in Table 1. All of these oligonucleotides were synthesized at Integrated DNA Technologies.

[0053] Construction of Phages and Plasmids--

[0054] M13.DELTA.linker5 was constructed by treating DNA of M13tg130 (GenBank Accession No. L08828) with restriction endonucleases PstI and EcoRI, followed by treatment with DNA polymerase T4 and DNA ligase.

[0055] M13Cm3 was constructed by cloning a fragment of the chloramphenicol acetyl transferase-encoding fragment plasmid pACYC 184 (GenBank Accession No. X06403) PCR-amplified with primers Cm-Bsp119I and Cm-NatrI (Table 1) and treated with restriction endonucleases Bsp119I and Nan into the DNA of M13.DELTA.linker5 treated with restriction endonuclease NarI.

[0056] M13CmSompbioVII8 was constructed by substituting the 461 bp PagI-Bsp1407I fragment in M12Cm3 with a PCR-amplified fragment of DNA that was created via PCR-mediated joining and amplification of four fragments. The first and the fourth of the four fragments were amplified from M13Cm3 using pairs of primers VII-N5', VII-N3' and VII-05', VII-C3', respectively. The second fragment encoding signal peptide of E. coli, OmpA, was amplified from E. coli chromosome DNA using primers S-5' and S-3'. The third fragment, encoding a biotinylatable peptide, was amplified from plasmid pParaBAD-bio6 with primers Bio-52' and Bio-3'.

[0057] Plasmid pParaBAD-bio6 was created earlier in our lab and contains a synthetic fragment encoding biotinylatable peptide and formed by oligonucleotides Bio-Nco, Bio-Nco-P, Bio-Nde and Bio-Nde-P. The sequence of pParaBAD-bio6 is given in Table 4.

[0058] M13CmSompbioTAGVII-1 was constructed by substitution of the 33 bp NotI-I-SceI fragment in DNA of M13CmSompbioVII8 with a DNA fragment formed by oligonucleotides SompTAG5' and SompTAG-3'.

[0059] M13CmSompbioTAGVII1mut1 was generated via site specific mutagenesis of M13CmSompbioTAGVII-1 with primers TAG-I and TAG-II2.

[0060] M13CmSomp.DELTA.TAGVII10 was constructed by substitution of the 98 bp XhoI-Cfr42I fragment in M13CmSompbioTAGVII1mut1 with DNA formed by oligonucleotides M13delBio-5' and M13delBio-3'.

[0061] Plasmid pGEM-LuxC2 was constructed by cloning a fragment amplified from Photorhabdus luminescence chromosome (GenBank Accession No. AF403784) by PCR with primers P.luc-5'C and P.luc-3'-Hb into pGEM-T Easy Vector (Promega). Luciferase-encoding sequences in this plasmid are placed under control of a lactose promoter.

[0062] M13LuxABE2 was constructed by cloning a 3725 bp EcoRI fragment of plasmid pGEM-LuxC2 into the EcoRI site of vector M13tg130. Luciferase-encoding sequences in this construct are under control of the lactose promoter.

[0063] M13LuxABElink15 was generated by substitution of the 1171 bp Bsp1407I-CaiI fragment of M13LuxABE2 with a Bsp1407I-CaiI fragment of the same phage that was generated via a two-step process. First, two separate fragments were amplified by PCR using two pairs of primers: VII-N5', S-IIIC and IIIN, VII-N3', respectively. Second, two amplified fragments were joined together and amplified in a separate PCR using primers VII-N5' and VII-N3'.

[0064] M13LuxABESstrep25-1 was constructed by joining a 1117 bp CaiI-Bsp1407I fragment of M13LuxABE2 with a 1303 bp CaiI-Bsp1407I fragment of M13CmSstrepVII25, which was isolated from phage display MD12.TM. and has an affinity to streptavidin.

[0065] M13LuxABESspAGVII1 was constructed by joining the 1171 bp CaiI-Bsp1407I fragment of M13LuxABE2 with the 1303 bp CaiI-Bsp1407I fragment of M13CmSspAGVII12, which was isolated from phage display MD12.TM. and has an affinity to streptococcal protein G.

[0066] M13LuxABElink-SompbioVII3 was constructed by joining the 9000 bp Eco105I-Bsu36I fragment of M13LuxABElink15 with the 2194 bp Bsu36I-Eco105I fragment of M13CmSompbioVII8.

[0067] pParaBAD-bioLuxABE2 was constructed by joining the 3626 bp luciferase-encoding Bsp68I-PstI fragment of phage M13LuxABE2 and the 4813 bp SmaI-PstI fragment of plasmid pParaBAD-bio6.

[0068] pParaBAD-bioLuxAB7 was generated by treating pParaBAD-bioLuxABE2 DNA with restriction endonucleases Ecl13611 and BstXI, followed by treatment with DNA polymerase T4 and ligase.

[0069] T71-2aLuxAB was constructed by combining fragments of phage T7Select1-2b (Novagen) and plasmid pParaBAD-bioLuxAB7. The fragment of phage T7Select1-2b was generated as a result of treatment of the phage DNA with restriction endonuclease BamHI and DNA polymerase T4, followed by treatment with EcoRI. The fragment of plasmid pParaBAD-bioLuxAB7 was generated as a result of treatment of the plasmid DNA with restriction endonuclease XhoI and DNA polymerase T4, followed by treatment with restriction endonuclease EcoRI.

[0070] pSL-LuxABE10 was constructed by inserting the luciferase-encoding PvuII fragment of plasmid pGEM-LuxC2 into the DNA of phasmid pSL-EGFP2, which had been treated with restriction endonuclease Pfl23II and DNA polymerase T4. Phasmid pSL-EGFP2 was constructed earlier in our lab and contains the origin of replication of plasmid pMB1, the sequence encoding .beta.-lactamase, and a fragment of bacteriophage lambda genome that encodes proteins required for maintenance of the phage's lysogenic state, as well as proteins required for propagation via lytic state. The sequence of pSL-EGFP2 is given in Table 5.

[0071] pTn-I-Sce2-PsapLuxAB-Cm1 was constructed earlier in our lab on the basis of commercially available vector pMOD-2<MCS>EZ::TN. This plasmid contains a hybrid operon encoding both subunits of luciferase from P. luminescence and chloramphenicol acetyl transferase and controlled by sapA promoter of the Campylobacter fetus. This operon is positioned between inverted repeats that are recognized by transposase Tn5 and, together with these repeats, forms a mini-transposon. The sequence of pTn-I-Sce2-PsapLuxAB-Cm1 is given in Table 6.

[0072] .lamda.::Tn-I-Sce2-PsapLuxAB-Cm5 was generated by in vitro transposition of the luciferase-encoding mini-transposon into the genome of phage .lamda.CI857Sam7. The mini-transposon was excised from plasmid pTn-I-Sce2-PsapLuxAB-Cm1 using restriction endonuclease BoxI. After completion of the in vitro transposition reaction using EZ-Tn5.TM. Transposase as suggested by the manufacturer (Epicentre, Madison), the mixture was packaged using MaxPlax.TM. Lambda Packaging Extract (Epicentre, Madison). The packaged DNA was introduced into E. coli LE392. This strain was used as a host for further propagation of .lamda.::Tn-I-Sce2-PsapLuxAB-Cm5.

[0073] Conjugation of T71-2aLuxAB with spAG--

[0074] Phage T71-2aLuxAB, purified on Cs-gradient, was dialyzed against 20 mM sodium phosphate, 150 mM NaCl, and 1 mM EDTA, pH7.5 (PBS-EDTA). 100 .mu.l of such a suspension, containing 2.times.10.sup.12 plaque forming units, were combined with 5 .mu.l of 20 mM Sulfo-LC-SPDP (Thermo Scientific) and the mixture was incubated for 30 min at room temperature. Then, reaction byproducts were removed from the reaction mixture by passing the reaction mixture through a desalting column equilibrated with PBS-EDTA. The collected sample of Sulfo-LC-SPDP modified T71-2aLuxAB was combined with an equal volume of solution containing 2.1 mg/ml of hybrid protein spAG. This protein was constructed earlier in our lab and includes immunoglobulin binding domains of staphylococcal protein A and streptococcal protein G, as well as a single cysteine residue per molecule in its structure. After overnight incubation at room temperature, the reaction mixture was subjected to ultrafiltration on Centricon.RTM. Ultracel YM-100(Millipore) to separate the T71-2aLuxAB-spAG conjugate from free spAG.

[0075] Construction of MD12.TM. Phage Display and its Screening--

[0076] M13 phage-based display MD12.TM. was constructed via site-specific mutagenesis of M13CmSomp.DELTA.TAGVII10 using the primer and procedure of Scholle, et al. The length of the randomized sequence was 12 amino acid residues and the resulting diversity of the library was 10.sup.11. This library was used for selection of sequences with an affinity to streptavidin and the hybrid between staphylococcal protein A and streptococcal protein G. Both of these proteins had Ni affinity tags incorporated into their structure. Therefore, for the purpose of biopanning, these proteins were immobilized on Ni-carrying magnetic beads--MagneHis.TM. (Promega) and in this form were exposed to MD12.TM. display. Otherwise, the biopanning was carried out according to standard procedure described by Smith.

[0077] Biotinylation of Phage M13LuxABElink-SompbioVII3--

[0078] The reaction containing 10.sup.12 phage particles and 5 .mu.g of recombinant biotin ligase was carried out in a buffer containing 40 mM TrisHCl, pH 8.0, 100 mM KCl, 3 mM ATP, 5.5 mM MgCl.sub.2 and 5 mM biotin. The total volume was 500 .mu.l and the reaction mixture was incubated overnight at 37.degree. C. Upon completion of the reaction, the mixture was dialyzed against three changes of PBS to remove unincorporated biotin, as well as ATP.

[0079] PLISA--

[0080] subsequent dilutions of analyzed recombinant proteins (streptavidin, BoNT/A-CH5 or SpAG) in PBS were loaded into wells of a 96-well plate and plates were incubated at 4.degree. C. overnight. Then, the solution was removed from wells and wells were filled with corresponding blocking solution. In the case of streptavidin and BoNT/A-CH,5 we used normal horse serum from VectaStain ABC kit (Vector Laboratories Inc., CA). In the case of SpAG, we used BSA. Blocking was conducted at 37.degree. C. for 1 hour. Then, wells were washed three times with PBS containing 0.1% Tween20 (PBST) and 50 .mu.l aliquots of PBST containing the appropriate detector phage at concentration 10.sup.9 phage particles per milliliter were added to each well. After a 1 hour-long incubation at 37.degree. C., the liquid was removed from wells and wells were washed ten times with PBST and one time with PBS. Then, 200 .mu.l of overnight ER2738 culture diluted in fresh LB media supplemented with IPTG were added to each well and plates were placed in the 37.degree. C. incubator. At appropriate time points, 50 .mu.l aliquots were transferred into wells of an opaque 96-well Microlite.TM.2 .mu.late (Thermo Scientific) and supplemented with 25 .mu.l of 0.004% of decanal in water. Luminescence was detected using Veritas luminometer (Promega).

Results

[0081] Construction and properties of the luciferase-encoding phage. To demonstrate that bacterial luciferase can be used to speed up detection of phages, we created luciferase-encoding derivatives of three different E. coli phages: M13, T7 and .lamda..

[0082] As a donor for the luciferase-encoding sequence, we used plasmid pGEM-LuxABE2. This plasmid was constructed in our lab and, in addition to sequences luxA and B, encodes subunits of bacterial luciferase containing luxE from P. luminescence. Previously, we found that the presence of luxE stimulates luminescence of E. coli cells possessing luxA and B (unpublished data).

[0083] Derivatives of three chosen phages, named M13LuxABE2, T71-2aLuxAB, and pSL-LuxABE10, respectively, were generated using known structure of phages' genomes and conventional genetic engineering techniques as described in Materials and Methods. Phages M13LuxABE2 and pSL-LuxABE10 carry the luxABE portion of the lux operon. At the same time, because of the limitations caused by the size of the genome of the used phage T7 derivatives and capacity of phage capsid we had to limit portion of the lux operon present in T71-2aLuxAB just to luxAB. The sequences of M13LuxABE2 (SEQ ID NO:1), T71-2aLuxAB (SEQ ID NO:2), and pSL-LuxABE10 (SEQ ID NO:3) are given in Table 7, Table 8, and Table 9, respectively.

[0084] Also, we constructed a luciferase-encoding derivative of bacteriophage .lamda. called .lamda.::Tn-I-Sce2-PsapLuxAB-Cm5, using transposition in vitro. Construction of this derivative may serve as a demonstration of how the luciferase-encoding form of any DNA-containing phage can be developed even when knowledge of its genome structure is limited or not available. The sequence of .lamda.::Tn-I-Sce2-PsapLuxAB-Cm5 (SEQ ID NO:4) is given in Table 10.

[0085] Analysis revealed that all developed luciferase-encoding phages cause production of light whose intensities are proportional to the number of phage particles used in the experiment (FIGS. 1 and 2). However, in the case of phage T7 derivatives, after a relatively short period during which the light intensity grows, the second period starts during which the light intensity begins to decrease. This occurs because, in the course of its development, phage T7 kills host cells and causes their lysis. Consequently, the amount of luciferase accumulated by the cell population stops growing and the efficiency of the luciferase itself starts to decrease, probably, because the concentration of FMNH.sub.2 outside the cell is lower than that required for the reaction.

[0086] The derivative of phage .lamda. used in the experiment presented in FIG. 1 is capable of propagating either through lytic or lysogenic pathways. It contains a temperature-sensitive repressor CI. The experiment reported in FIG. 1 was conducted at 37.degree. C. At this temperature, the phage predominantly propagates via lytic pathway. However, lysis of host cells by this phage occurs less efficiently and takes more time than that caused by the phage T71-2aLuxAB. Consequently, light emission curves produced by pSL10-LuxABE10 are more stable over time and therefore more suitable for quantitative analysis.

[0087] To demonstrate that luciferase-encoding derivatives even of lytic phages such as T7 can be used as signal amplifying devices in immunologic reactions, we conjugated T71-2aLuxAB with a hybrid protein composed of immunoglobulin-binding domains of staphylococcal protein A and streptococcal protein G (spAG). Data presented in FIG. 3 demonstrate that this conjugate (T71-2aLuxAB--spAG) can detect the presence of rabbit serum diluted 10.sup.6-10.sup.7 times.

[0088] Among all tested luciferase-encoding phages, M13LuxABE2 generated the most suitable light emission curves for quantitative analysis. This phage that does not kill host cells and light emission by cultures infected with this phage does not experience dramatic drops characteristic of cultures infected with lytic phages. As demonstrated in FIG. 2 panel A, less than 100 phage particles of M13LuxABE2 can be detected using AU-DH10 culture within 4 hours of incubation. The luminescence emitted by the infected culture is proportional to the number of phages used for the initial infection. The number of phages that can be reliably quantified under used conditions spans between 10.sup.2 and 10.sup.7 and the results of quantification are not dramatically affected by the original dilution of the host culture. Also, we tested four F-factor-carrying cultures for the ability to serve as hosts for the M13LuxABE2 phage. Data presented in FIG. 2 panel B demonstrate that all tested strains were capable of supporting growth of M13LuxABE2 and accumulated enzymatically active luciferase. However, in different strains, this accumulation occurred with different speed. Among all tested strains ER2738 had the highest speed of luciferase accumulation. This strain was used as a host in all further PLISA.

[0089] Construction of MD12.TM. Phage Display and Generation of Detectors for Streptavidin and SpAG.

[0090] To create phages capable of detecting target molecules of interest, we had to incorporate antigen-recognizing moieties into the structure of the phage capsid. Phage display technology allows to do this, but the most commonly used approach through exposure of antigen-specific sequences as part of protein III requires dissociation of the phage particle from the antigen prior to infection of the host cell. Our goal was to create phages that could infect host cells while being attached to the antigen. For this reason, we decided to use protein VII--not involved in receptor recognition--as a carrier of antigen-recognizing polypeptides. Through the set of cloning experiments described in Materials and Methods, we assembled a derivative of vector M13tg130 called M13CmSomp.DELTA.TAGVII10. This derivative has sequences for chloramphenicol acetyl transferase, .alpha.-peptide of .beta.-galactosidase and hybrid protein VII. The latter encodes sequences for the signal peptide of E. coli OmpA and protein VII. These two sequences are separated by a linker possessing the TAG codon. Using the inability of M13CmSomp.DELTA.TAGVII10 to propagate on sup.sup.- strains, we constructed a phage display library of dodekapeptides by the procedure described by Scholle and coauthors.

[0091] The resulting MD12.TM. phage display was used for biopanning with SpAG and streptavidin and produced a number of phages targeted at the corresponding protein. We used two of them: M13CmSspAGVII12 and M13CmSstrepVII25 targeted at SpAG and streptavidin, respectively. These two phages were further modified as is described in Materials and Methods to produce phages targeted either at SpAG or streptavidin and simultaneously carry luxABE sequence (M13LuxABESspAGVII1 and M13LuxABESstrep25-1, respectively).

[0092] Data presented in FIG. 4 demonstrate that both phages were able to function as detectors of corresponding proteins. Sensitivities of the phages were slightly different, but both were in the range of 0.1-2 ng of target per well. We used phage M13LuxABESstrep25-1 to test whether the binding to immobilized antigen interferes with the phage's ability to infect host cells or not. First, we determined that a phage attached to immobilized streptavidin can be effectively released by addition of 5 mM biotin to wells. Then, we tested whether treatment of wells containing the immobilized streptavidin-phage sandwich with 5 mM biotin prior to addition of host cells changes results of the PLISA. As shown in FIG. 4 panel B, experiments with and without biotin give practically the same results, suggesting that release from the antigen is not required for infection to occur. The length of incubation of the phage with antigen, on the other hand, does matter. As depicted in FIG. 4 panel B, overnight incubation resulted in a stronger signal than 1 hour-long incubation, even at higher temperature. However, the observed difference was not dramatic enough to suggest that the protocol should include overnight incubation rather than a shorter one.

[0093] Construction and Detector Properties of Biotinylated Form of the Luciferase-Encoding Phage.

[0094] Data presented in FIG. 4 panel B and FIG. 5 demonstrate that PLISA utilizing phage M13LuxABESstrep25-1 for detection of streptavidin is about 20-100 times more sensitive than ELISA utilizing biotinylated horse radish peroxidase for detection of streptavidin. However, the affinity of peptide exposed on the capsid of phage M13LuxABESstrep25-1 to streptavidin is substantially lower than that of biotin. To compare sensitivities of PLISA and ELISA in comparable conditions, we assembled phage M13LuxABElink-SompbioVII3 (see Materials and Methods). This phage exposes on its capsid a polypeptide that serves as a substrate for biotin ligase (BirA). Although E. coli strain AU-DH10 has birA gene, it seems that the majority of phage particles produced in this strain remain unmodified. The ratio of biotinylated phage particles increased dramatically after incubation of these phages with purified BirA in the presence of free biotin and ATP. The sensitivity of streptavidin detection grows along with this increase. As shown in FIG. 5, the biotinylated form of M13LuxABElink-SompbioVII3 can detect as little as 1 pg loaded in the well (the amount immobilized is probably lower).

[0095] To test whether use of M13LuxABElink-SompbioVII3 can increase sensitivity of detection of molecules other than streptavidin, we tested it in the system for detection of fragments of botulinum neurotoxin. This system includes rabbit antibodies specific to botulinum neurotoxin A, biotinylated anti-rabbit antibodies, streptavidin and biotinylated horse radish peroxidase used in consecutive order. As shown in FIG. 6, substitution of the last element in this system with biotinylated M13LuxABElink-SompbioVII3 allowed to increase sensitivity of detection by at least 10 times.

[0096] Luciferase-encoding phages have numerous advantages of a signal amplification device. They are capable of exponential amplification while producing an easily detectable and quantifiable light signal. Also, the cost of their production and use is substantially lower than that of any enzyme-based signal amplifying device currently used in immuno assays. Data presented herein demonstrate that via physical connection with antigen-recognizing moieties, such phages can be converted into very sensitive and simple to use detectors. Even with relatively low affinity ligands, such as peptides isolated from a phage display library, it is possible to build phage-based detector systems that will have higher sensitivity than conventional systems, despite the fact that conventional systems utilize ligands with higher affinity to the antigen (FIG. 4 panel B and FIG. 3). The higher affinity to the antigen of the moiety exposed on the phage surface, the higher the sensitivity of the resulting construct will be. Accordingly, in some embodiments short polypeptides having higher affinity to a chosen antigen are employed, and/or 2) chemical coupling or coupling via chimeras, such as streptavidin-SpAG, of conventional antibodies or their derivatives to phage particles are utilized, and/or 3) single chain antibodies or variable domains of heavy chain only camelidae antibodies that can be incorporated into the phage capsid as a result of genetic modifications of the phage genome are used.

[0097] In some embodiments, phage derivatives that can directly target antigens of interest are provided. In some embodiments, this approach will not only increase sensitivity of PLISA, but will simplify it and make it less expensive. Although both lytic and temperate phages can be used as signal amplifying devices, phages that can propagate without causing lysis of host cells will have an advantage, in some embodiments, because of a more stable signal whose changes are easier to interpret. Data presented in FIG. 2 demonstrate that in the case of M13 derivatives, the range of quantitative response spans over five logarithms of phage concentrations. This range is substantially wider than that of enzymes traditionally used in immuno assays. The less inclined slope of the PLISA curve than that of ELISA in FIG. 5 may serve as a demonstration of an additional advantage that use of luciferase-encoding derivatives of M13 devices in immuno assay may provide.

TABLE-US-00001 TABLE 1 List of oligonucleotides used in the project. Name Sequence Used for Cm-Bsp119I 5'-gctttcgaatttctgccattcatcc Construction of Cm-NarI 5'-ttgggcgccatctccttgggccaacttttggcgaaaatg M13Cm3 S-5' 5'-cgttccggctaagtaacatgaaaaagacagctatcgcgattgcagtg Construction of S-3' 5'-tgcccgcggcctgcg M13 CmSompbi VII-N5' 5'-gcgcctggtctgtacaccgttcatctg oVII8 VII-N3' 5'-catgttacttagccggaacg Bio-52' 5'-cgcaggccgcgggcgcaagcagcctgagaca Bio-3' 5'-tgcggccgcctgggccccct VII-C5' 5'-agggggcccaggcggccgcagagcaggtcgcggatt VII-C3' 5'-aagcgcagtctctgaattta Bio-Nco 5'-catggcaagcagcctgagacagattctggactcccagaaaatggagtgga Assembly of Bio-Nco-P 5'-ggtccaacgccgggggcagcggtagggataacagggtaatcca sequence Bio-Nde 5'-tatggattaccctgttatccctaccgctgcccccggcgttg encoding Bio-Nde-P 5'-gacctccactccattttctgggagtccagaatctgtctcaggctgcttgc biotinylatable peptide TAG-I 5'-ctattttgcacccagctacaattttatcctgaatcttaccaacgc Construction of TAG-II2 5'-ggcttagagcttaattgctgaatctggtgctgtagctca M13 CmSompbi oTAGVII1mut1 M13 5'-ggggagctctgggggcagcggtagggataacccctcaggctagatgc Construction of delBio-5' M13 CmSomp.DELTA. TAGVII10 M13 5'-tcgagcatctagcctgaggggttatccctaccgctgcccccagagctccccgc Construction of delBio-3' pGEM-LuxC2 P.luc-5'C 5'-atggtaaagcaagatgaagttatcacattgt P.luc-3'-Hb 5'-atgtcaactattaaatgcttggtttaag Construction of VII-N5' 5'-gcgcctggtctgtacaccgttcatctg M13LuxABElin k15 S-IIIC 5'-ctcgagtcggccgcccatggcaacagtttcagcggagtga Construction of IIIN 5'-ccatgggcggccgactcgaggaaagttgtttagcaaaacccc MD12 .TM. VII-N3' 5'-catgttacttagccggaacg VII-N12cor 5'-cgacctgctctgcggccgccga(N).sub.36accgctgccccc

[0098] This example demonstrates that the methods and compositions described herein are useful in the detection of molecules.

TABLE-US-00002 TABLE 2 Sequence of SpAG. mgsshhhhhhssglvprgshrstledpsqstnvlgeakklnesqapkadn nfnkeqqnafyeilnmpnlneeqrngfiqslkddpsqsanllaeakklne sqapkadnkfnkeqqnafyeilhlpnlneeqrngfiqslkddpsqsanll aeakklndaqapkadnkfnkeqqnafyeilhlpnlteeqrngfiqslkdd prstlaaasgastdtyklilngktlkgettteavdaataekvfkqyandn gvdgewtyddatktftvtekpevidaseltpavttyklvingktlkgett tkavdaetaekafkqyandngvdgvwtyddatktftvtemvtevpvvrgg scg

TABLE-US-00003 TABLE 3 Sequence of BoNT/A-CH5. msackgmgsshhhhhhssglvprgshmarivdnqrllstfteyikniint silnlryesnhlidlsryaskinigskvnfdpidknqiqlfnlesskiev ilknaivynsmyenfstsfwiripkyfnsislnneytiincmennsgwkv slnygeiiwtlqdtqeikqrvvfkysqminisdyinrwifvtitnnrlnn skiyingrlidqkpisnlgnihasnnimfkldgcrdthryiwikyfnlfd kelnekeikdlydnqsnsgilkdfwgdylqydkpyymlnlydpnkyvdvn nvgirgymylkgprgsvmttniyinsslyrgtkfiikkyasgnkdnivrn ndrvyinvvvknkeyrlatnasqagvekilsaleipdvgnlsqvvvmksk ndqgitnkckmnlqdnngndigfigfhqfnniaklvasnwynrqierssr tlgcswefipvddgwgerpl

TABLE-US-00004 TABLE 4 Sequence of pParaBAD-bio6. agatctgcaagtcttgcataatgtgcctgtcaaatggacgaagcagggat tctgcaaaccctatgctactccgtcaagccgtcaattgtctgattcgtta ccaattatgacaacttgacggctacatcattcactttttcttcacaaccg gcacggaactcgctcgggctggccccggtgcattttttaaatacccgcga gaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgatag gcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcc tcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtga cagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaa aattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacc cgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccg cagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcc cttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgc ggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattga cggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaaccc actggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatc tctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcg tccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatat aacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttg gcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcc cggcagcaggggatcattttgcgcttcagccatacttttcatactcccgc cattcagagaagaaaccaattgtccatattgcatcagacattgccgtcac tgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttat taaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgta acaaaagtgtctataatcacggcagaaaagtccacattgattatttgcac ggcgtcacactttgctatgccatagcatttttatccataagattagcgga tcctacctgacgctttttatcgcaactctctactgtttctccatacccgt ttttttggatggagtgaaacgatggcgattgcaatttctagcgccattcg ccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttc gctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagtt gggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagt gaattcgagctagaaataattttgtttaactttaagaaggagatatacca tggcaagcagcctgagacagattctggactcccagaaaatggagtggagg tccaacgccgggggcagcggtagggataacagggtaatccatatgctcga gggggcccaggcggccgcactcgactcggtacccggggatcctctagagt cgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcct gtgtgaaattgttatccgctcacaattccacacaacatacgagccggaag cataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaa ttgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccag cgagctcgaattcggctgctaacaaagcccgaaaggaagctgagttggct gctgccaccgctgagcaataactagcataaccccttgggcctctaaacgg gtcttgaggggttttttgctgaaaggaggaactatatccggatcggagat caattctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaaca gttgcgtagcctgaatggcgaatgggacgcgccctgtagcggcgcattaa gcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagc gccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgtt cgccggctttccccgtcaagctctaaatcgggggctccctttagggttcc gatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgat ggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgac gttggagtccacgttctttaatagtggactcttgttccaaactggaacaa cactcaaccctatctcggtctattcttttgatttataagggattttgccg atttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgc gaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggg gaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaat atgtatccgctcatgagacaataaccctgataaatgcttcaataatattg aaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccct tttttgcggcattttgccgtcctgtttttgctcacccagaaacgctggtg aaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcga actggatctcaacagcggtaagatccttgagagttttcgccccgaagaac gttttccaatgatgagcacttttaaagttctgctatgtggcgcggtatta tcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattc tcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacgg atggcatgacagtaagagaattatgcagtgctgccataagcatgagtgat aacactgcggccaacttacttctgacaacgatcggaggaccgaaggagct aaccgctttttttcacaacatgggggatcatgtaactcgccttgatcgtt gggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacg atgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaact acttactctagcttcccggcaacaattaatagactggatggaggcggata aagttgcaggaccacttctgcgctcggcccttccggctggctggtttatt gctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagc actggggccagatggtaagccctcccgtatcgtagttatctacacgacgg gcagtcaggcaactatggatgaacgaaatagacagatcgctgagataggt gcctcactgattaagcattggtaactgtcagaccaagtttactcatatat actttagattgatttaaaacttcatttttaatttaaaaggatctaggtga agatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcg ttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgaga tcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgc taccagcggtggtttgtttgccggatcaagagctaccaactctttttccg aaggtaactggcttcagcagagcgcagataccaaatactgtccttctagt gtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacat acctcgctctgctaatcctgttaccagtggctgctgccagtggcgataag tcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgca gcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaa cgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgcc acgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggt cggaacaggagagcgcacgagggagcttccaggggggaacgcctggtatc tttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttg tgatgctcgtcaggggggccgagcctatggaaaaacgccagcaacgcggc ctttttacggttcctggccttttgctggccttttgctcacatgttctttc ctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtga gctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgag cgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgt gcggtatttcacaccgcatatatggtgcactctcagtacaatctgctctg atgccgcatagttaagccagtatacactccgctatcgctacgtgactgca aggagatggcgcccaacagtcccccggccacggggcctgccaccataccc acgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttcccc atcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccgg tgatgccggccacgatgcgtccggcgtagaggatcttg

TABLE-US-00005 TABLE 5 Sequence of pSL-EGFP2. gggcggcgacctcgcgggttttcgctatttatgaaaattttccggtttaa ggcgtttccgttcttcttcgtcataacttaatgtttttatttaaaatacc ctctgaaaagaaaggaaacgacaggtgctgaaagcgaggctttttggcct ctgtcgtttcctttctctgtttttgtccgtggaatgaacaatggaagtca acaaaaagcagctggctgacattttcggtgcgagtatccgtaccattcag aactggcaggaacagggaatgcccgttctgcgaggcggtggcaagggtaa tgaggtgctttatgactctgccgccgtcataaaatggtatgccgaaaggg atgctgaaattgagaacgaaaagctgcgccgggaggttgaagaactgcgg caggccagcgaggcagatctccagccaggaactattgagtacgaacgcca tcgacttacgcgtgcgcaggccgacgcacaggaactgaagaatgccagag actccgctgaagtggtggaaaccgcattctgtactttcgtgctgtcgcgg atcgcaggtgaaattgccagtattctcgacgggctccccctgtcggtgca gcggcgttttccggaactggaaaaccgacatgttgatttcctgaaacggg atatcatcaaagccatgaacaaagcagccgcgctggatgaactgataccg gggttgctgagtgaatatatcgaacagtcaggttaacaggctgcggcatt ttgtccgcgccgggcttcgctcactgttcaggccggagccacagaccgcc gttgaatgggcggatgctaattactatctcccgaaagaatccgcatacca ggaagggcgctgggaaacactgccctttcagcgggccatcatgaatgcga tgggcagcgactacatccgtgaggtgaatgtggtgaagtctgcccgtgtc ggttattccaaaatgctgctgggtgtttatgcctactttatagagcataa gcagcgcaacacccttatctggttgccgacggatggtgatgccgagaact ttatgaaaacccacgttgagccgactattcgtgatattccgtcgctgctg gcgctggccccgtggtatggcaaaaagcaccgggataacacgctcaccat gaagcgtttcactaatgggcgtggcttctggtgcctgggcggtaaagcgg caaaaaactaccgtgaaaagtcggtggatgtggcgggttatgatgaactt gctgcttttgatgatgatattgaacaggaaggctctccgacgttcctggg tgacaagcgtattgaaggctcggtctggccaaagtccatccgtggctcca cgccaaaagtgagaggcacctgtcagattgagcgtgcagccagtgaatcc ccgcattttatgcgttttcatgttgcctgcccgcattgcggggaggagca gtatcttaaatttggcgacaaagagacgccgtttggcctcaaatggacgc cggatgacccctccagcgtgttttatctctgcgagcataatgcctgcgtc atccgccagcaggagctggactttactgatgcccgttatatctgcgaaaa gaccgggatctggacccgtgatggcattctctggttttcgtcatccggtg aagagattgagccacctgacagtgtgacctttcacatctggacagcgtac agcccgttcaccacctgggtgcagattgtcaaagactggatgaaaacgaa aggggatacgggaaaacgtaaaaccttcgtaaacaccacgctcggtgaga cgtgggaggcgaaaattggcgaacgtccggatgctgaagtgatggcagag cggaaagagcattattcagcgcccgttcctgaccgtgtggcttacctgac cgccggtatcgactcccagctggaccgctacgaaatgcgcgtatggggat gggggccgggtgaggaaagctggctgattgaccggcagattattatgggc cgccacgacgatgaacagacgctgctgcgtgtggatgaggccatcaataa aacctatacccgccggaatggtgcagaaatgtcgatatcccgtatctgct gggatactggcgggattgacccgaccattgtgtatgaacgctcgaaaaaa catgggctgttccgggtgatccccattaaaggggcatccgtctacggaaa gccggtggccagcatgccacgtaagcgaaacaaaaacggggtttacctta ccgaaatcggtacggataccgcgaaagagcagatttataaccgcttcaca ctgacgccggaaggggatgaaccgcttcccggtgccgttcacttcccgaa taacccggatatttttgatctgaccgaagcgcagcagctgactgctgaag agcaggtcgaaaaatgggtggatggcaggaaaaaaatactgtgggacagc aaaaagcgacgcaatgaggcactcgactgcttcgtttatgcgctggcggc gctgcgcatcagtatttcccgctggcagctggatctcagtgcgctgctgg cgagcctgcaggaagaggatggtgcagcaaccaacaagaaaacactggca gattacgcccgtgccttatccggagaggatgaatgacgcgacaggaagaa cttgccgctgcccgtgcggcactgcatgacctgatgacaggtaaacgggt ggcaacagtacagaaagacggacgaagggtggagtttacggccacttccg tgtctgacctgaaaaaatatattgcagagctggaagtgcagaccggcatg acacagcgacgcaggggacctgcaggattttatgtatgaaaacgcccacc attcccacccttctggggccggacggcatgacatcgctgcgcgaatatgc cggttatcacggcggtggcagcggatttggagggcagttgcggtcgtgga acccaccgagtgaaagtgtggatgcagccctgttgcccaactttacccgt ggcaatgcccgcgcagacgatctggtacgcaataacggctatgccgccaa cgccatccagctgcatcaggatcatatcgtcgggtcttttttccggctca gtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgt gccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatga ctgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatgattc gggaaggtgtggccatgcacgcctttaacggtgaactgttcgttcaggcc acctgggataccagttcgtcgcggcttttccggacacagttccggatggt cagcccgaagcgcatcagcaacccgaacaataccggcgacagccggaact gccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattac gtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggat accccgtgagttacccggcgggcgcgcctcgttcattcacgtttttgaac ccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatg gagcagatgaagatgctcgacacgctgcagaacacgcagctgcagagcgc cattgtgaaggcgatgtatgccgccaccattgagagtgagctggatacgc agtcagcgatggattttattctgggcgcgaacagtcaggagcagcgggaa aggctgaccggctggattggtgaaattgccgcgtattacgccgcagcgcc ggtccggctgggaggcgcaaaagtaccgcacctgatgccgggtgactcac tgaacctgcagacggctcaggatacggataacggctactccgtgtttgag cagtcactgctgcggtatatcgctgccgggctgggtgtctcgtatgagca gctttcccggaattacgcccagatgagctactccacggcacgggccagtg cgaacgagtcgtgggcgtactttatggggcggcgaaaattcgtcgcatcc cgtcaggcgagccagatgtttctgtgctggctggaagaggccatcgttcg ccgcgtggtgacgttaccttcaaaagcgcgcttcagttttcaggaagccc gcagtgcctgggggaactgcgactggataggctccggtcgtatggccatc gatggtctgaaagaagttcaggaagcggtgatgctgatagaagccggact gagtacctacgagaaagagtgcgcaaaacgcggtgacgactatcaggaaa tttttgcccagcaggtccgtgaaacgatggagcgccgtgcagccggtctt aaaccgcccgcctgggcggctgcagcatttgaatccgggctgcgacaatc aacagaggaggagaagagtgacagcagagctgcgtaatctcccgcatatt gccagcatggcctttaatgagccgctgatgcttgaacccgcctatgcgcg ggttttcttttgtgcgcttgcaggccagcttgggatcagcagcctgacgg atgcggtgtccggcgacagcctgactgcccaggaggcactcgcgacgctg gcattatccggtgatgatgacggaccacgacaggcccgcagttatcaggt catgaacggcatcgccgtgctgccggtgtccggcacgctggtcagccgga cgcgggcgctgcagccgtactcggggatgaccggttacaacggcattatc gcccgtctgcaacaggctgccagcgatccgatggtggacggcattctgct cgatatggacacgcccggcgggatggtggcgggggcatttgactgcgctg acatcatcgcccgtgtgcgtgacataaaaccggtatgggcgcttgccaac gacatgaactgcagtgcaggtcagttgcttgccagtgccgcctcccggcg tctggtcacgcagaccgcccggacaggctccatcggcgtcatgatggctc acagtaattacggtgctgcgctggagaaacagggtgtggaaatcacgctg atttacagcggcagccataaggtggatggcaacccctacagccatcttcc ggatgacgtccgggagacactgcagtcccggatggacgcaacccgccaga tgtttgcgcagaaggtgtcggcatataccggcctgtccgtgcaggttgtg ctggataccgaggctgcagtgtacagcggtcaggaggccattgatgccgg actggctgatgaacttgttaacagcaccgatgcgatcaccgtcatgcgtg atgcactggatgcacgtaaatcccgtctctcaggagggcgaatgaccaaa gagactcaatcaacaactgtttcagccactgcttcgcaggctgacgttac tgacgtggtgccagcgacggagggcgagaacgccagcgcggcgcagccgg acgtgaacgcgcagatcaccgcagcggttgcggcagaaaacagccgcatt atggggatcctcaactgtgaggaggctcacggacgcgaagaacaggcacg cgtgctggcagaaacccccggtatgaccgtgaaaacggcccgccgcattc tggccgcagcaccacagagtgcacaggcgcgcagtgacactgcgctggat cgtctgatgcagggggcaccggcaccgctggctgcaggtaacccggcatc tgatgccgttaacgatttgctgaacacaccagtgtaagggatgtttatga cgagcaaagaaacctttacccattaccagccgcagggcaacagtgacccg gctcataccgcaaccgcgcccctagaccttcatcactaaaggccgcctgt gcggctttttttacgggatttttttatgtcgatgtacacaaccgcccaac tgctggcggcaaatgagcagaaatttaagtttgatccgctgtttctgcgt ctctttttccgtgagagctatcccttcaccacggagaaagtctatctctc acaaattccgggactggtaaacatggcgctgtacgtttcgccgattgttt ccggtgaggttatccgttcccgtggcggctccacctctgaatttacgccg ggatatgtcaagccgaagcatgaagtgaatccgcagatgaccctgcgtcg

cctgccggatgaagatccgcagaatctggcggacccggcttaccgccgcc gtcgcatcatcatgcagaacatgcgtgacgaagagctggccattgctcag gtcgaagagatgcaggcagtttctgccgtgcttaagggcaaatacaccat gaccggtgaagccttcgatccggttgaggtggatatgggccgcagtgagg agaataacatcacgcagtccggcggcacggagtggagcaagcgtgacaag tccacgtatgacccgaccgacgatatcgaagcctacgcgctgaacgccag cggtgtggtgaatatcatcgtgttcgatccgaaaggctgggcgctgttcc gttccttcaaagccgtcaaggagaagctggatacccgtcgtggctctaat tccgagctggagacagcggtgaaagacctgggcaaagcggtgtcctataa ggggatgtatggcgatgtggccatcgtcgtgtattccggacagtacgtgg aaaacggcgtcaaaaagaacttcctgccggacaacacgatggtgctgggg aacactcaggcacgcggtctgcgcacctatggctgcattcaggatgcgga cgcacagcgcgaaggcattaacgcctctgcccgttacccgaaaaactggg tgaccaccggcgatccggcgcgtgagttcaccatgattcagtcagcaccg ctgatgctgctggctgaccctgatgagttcgtgtccgtacaactggcgta atcatggcccttcggggccattgtttctctgtggaggagtccatgacgaa agatgaactgattgcccgtctccgctcgctgggtgaacaactgaaccgtg atgtcagcctgacggggacgaaagaagaactggcgctccgtgtggcagag ctgaaagaggagcttgatgacacggatgaaactgccggtcaggacacccc tctcagccgggaaaatgtgctgaccggacatgaaaatgaggtgggatcag cgcagccggataccgtgattctggatacgtctgaactggtcacggtcgtg gcactggtgaagctgcatactgatgcacttcacgccacgcgggatgaacc tgtggcatttgtgctgccgggaacggcgtttcgtgtctctgccggtgtgg cagccgaaatgacagagcgcggcctggccagaatgcaataacgggaggcg ctgtggctgatttcgataacctgttcgatgctgccattgcccgcgccgat gaaacgatacgcgggtacatgggaacgtcagccaccattacatccggtga gcagtcaggtgcggtgatacgtggtgtttttgatgaccctgaaaatatca gctatgccggacagggcgtgcgcgttgaaggctccagcccgtccctgttt gtccggactgatgaggtgcggcagctgcggcgtggagacacgctgaccat cggtgaggaaaatttctgggtagatcgggtttcgccggatgatggcggaa gttgtcatctctggcttggacggggcgtaccgcctgccgttaaccgtcgc cgctgaaagggggatgtatggccataaaaggtcttgagcaggccgttgaa aacctcagccgtatcagcaaaacggcggtgcctggtgccgccgcaatggc cattaaccgcgttgcttcatccgcgatatcgcagtcggcgtcacaggttg cccgtgagacaaaggtacgccggaaactggtaaaggaaagggccaggctg aaaagggccacggtcaaaaatccgcaggccagaatcaaagttaaccgggg ggatttgcccgtaatcaagctgggtaatgcgcgggttgtcctttcgcgcc gcaggcgtcgtaaaaaggggcagcgttcatccctgaaaggtggcggcagc gtgcttgtggtgggtaaccgtcgtattcccggcgcgtttattcagcaact gaaaaatggccggtggcatgtcatgcagcgtgtggctgggaaaaaccgtt accccattgatgtggtgaaaatcccgatggcggtgccgctgaccacggcg tttaaacaaaatattgagcggatacggcgtgaacgtcttccgaaagagct gggctatgcgctgcagcatcaactgaggatggtaataaagcgatgaaaca tactgaactccgtgcagccgtactggatgcactggagaagcatgacaccg gggcgacgttttttgatggtcgccccgctgtttttgatgaggcggatttt ccggcagttgccgtttatctcaccggcgctgaatacacgggcgaagagct ggacagcgatacctggcaggcggagctgcatatcgaagttttcctgcctg ctcaggtgccggattcagagctggatgcgtggatggagtcccggatttat ccggtgatgagcgatatcccggcactgtcagatttgatcaccagtatggt ggccagcggctatgactaccggcgcgacgatgatgcgggcttgtggagtt cagccgatctgacttatgtcattacctatgaaatgtgaggacgctatgcc tgtaccaaatcctacaatgccggtgaaaggtgccgggaccaccctgtggg tttataaggggagcggtgacccttacgcgaatccgctttcagacgttgac tggtcgcgtctggcaaaagttaaagacctgacgcccggcgaactgaccgc tgagtcctatgacgacagctatctcgatgatgaagatgcagactggactg cgaccgggcaggggcagaaatctgccggagataccagcttcacgctggcg tggatgcccggagagcaggggcagcaggcgctgctggcgtggtttaatga aggcgatacccgtgcctataaaatccgcttcccgaacggcacggtcgatg tgttccgtggctgggtcagcagtatcggtaaggcggtgacggcgaaggaa gtgatcacccgcacggtgaaagtcaccaatgtgggacgtccgtcgatggc agaagatcgcagcacggtaacagcggcaaccggcatgaccgtgacgcctg ccagcacctcggtggtgaaagggcagagcaccacgctgaccgtggccttc cagccggagggcgtaaccgacaagagctttcgtgcggtgtctgcggataa aacaaaagccaccgtgtcggtcagtggtatgaccatcaccgtgaacggcg ttgctgcaggcaaggtcaacattccggttgtatccggtaatggtgagttt gctgcggttgcagaaattaccgtcaccgccagttaatccggagagtcagc gatgttcctgaaaaccgaatcatttgaacataacggtgtgaccgtcacgc tttctgaactgtcagccctgcagcgcattgagcatctcgccctgatgaaa cggcaggcagaacaggcggagtcagacagcaaccggaagtttactgtgga agacgccatcagaaccggcgcgtttctggtggcgatgtccctgtggcata accatccgcagaagacgcagatgccgtccatgaatgaagccgttaaacag attgagcaggaagtgcttaccacctggcccacggaggcaatttctcatgc tgaaaacgtggtgtaccggctgtctggtatgtatgagtttgtggtgaata atgcccctgaacagacagaggacgccgggcccgcagagcctgtttctgcg ggaaagtgttcgacggtgagctgagttttgccctgaaactggcgcgtgag atggggcgacccgactggcgtgccatgcttgccgggatgtcatccacgga gtatgccgactggcaccgcttttacagtacccattattttcatgatgttc tgctggatatgcacttttccgggctgacgtacaccgtgctcagcctgttt ttcagcgatccggatatgcatccgctggatttcagtctgctgaaccggcg cgaggctgacgaagagcctgaagatgatgtgctgatgcagaaagcggcag ggcttgccggaggtgtccgctttggcccggacgggaatgaagttatcccc gcttccccggatgtggcggacatgacggaggatgacgtaatgctgatgac agtatcagaagggatcgcaggaggagtccggtatggctgaaccggtaggc gatctggtcgttgatttgagtctggatgcggccagatttgacgagcagat ggccagagtcaggcgtcatttttctggtacggaaagtgatgcgaaaaaaa cagcggcagtcgttgaacagtcgctgagccgacaggcgctggctgcacag aaagcggggatttccgtcgggcagtataaagccgccatgcgtatgctgcc tgcacagttcaccgacgtggccacgcagcttgcaggcgggcaaagtccgt ggctgatcctgctgcaacagggggggcaggtgaaggactccttcggcggg atgatccccatgttcagggggcttgccggtgcgatcaccctgccgatggt gggggccacctcgctggcggtggcgaccggtgcgctggcgtatgcctggt atcagggcaactcaaccctgtccgatttcaacaaaacgctggtcctttcc ggcaatcaggcgggactgacggcagatcgtatgctggtcctgtccagagc cgggcaggcggcagggctgacgtttaaccagaccagcgagtcactcagcg cactggttaaggcgggggtaagcggtgaggctcagattgcgtccatcagc cagagtgtggcgcgtttctcctctgcatccggcgtggaggtggacaaggt cgctgaagccttcgggaagctgaccacagacccgacgtcggggctgacgg cgatggctcgccagttccataacgtgtcggcggagcagattgcgtatgtt gctcagttgcagcgttccggcgatgaagccggggcattgcaggcggcgaa cgaggccgcaacgaaagggtttgatgaccagacccgccgcctgaaagaga acatgggcacgctggagacctgggcagacaggactgcgcgggcattcaaa tccatgtgggatgcggtgctggatattggtcgtcctgataccgcgcagga gatgctgattaaggcagaggctgcgtataagaaagcagacgacatctgga atctgcgcaaggatgattattttgttaacgatgaagcgcgggcgcgttac tgggatgatcgtgaaaaggcccgtcttgcgcttgaagccgcccgaaagaa ggctgagcagcagactcaacaggacaaaaatgcgcagcagcagagcgata ccgaagcgtcacggctgaaatataccgaagaggcgcagaaggcttacgaa cggctgcagacgccgctggagaaatataccgcccgtcaggaagaactgaa caaggcactgaaagacgggaaaatcctgcaggcggattacaacacgctga tggcggcggcgaaaaaggattatgaagcgacgctgaaaaagccgaaacag tccagcgtgaaggtgtctgcgggcgatcgtcaggaagacagtgctcatgc tgccctgctgacgcttcaggcagaactccggacgctggagaagcatgccg gagcaaatgagaaaatcagccagcagcgccgggatttgtggaaggcggag agtcagttcgcggtactggaggaggcggcgcaacgtcgccagctgtctgc acaggagaaatccctgctggcgcataaagatgagacgctggagtacaaac gccagctggctgcacttggcgacaaggttacgtatcaggagcgcctgaac gcgctggcgcagcaggcggataaattcgcacagcagcaacgggcaaaacg ggccgccattgatgcgaaaagccgggggctgactgaccggcaggcagaac gggaagccacggaacagcgcctgaaggaacagtatggcgataatccgctg gcgctgaataacgtcatgtcagagcagaaaaagacctgggcggctgaaga ccagcttcgcgggaactggatggcaggcctgaagtccggctggagtgagt gggaagagagcgccacggacagtatgtcgcaggtaaaaagtgcagccacg cagacctttgatggtattgcacagaatatggcggcgatgctgaccggcag tgagcagaactggcgcagcttcacccgttccgtgctgtccatgatgacag aaattctgcttaagcaggcaatggtggggattgtcgggagtatcggcagc

gccattggcggggctgttggtggcggcgcatccgcgtcaggcggtacagc cattcaggccgctgcggcgaaattccattttgcaaccggaggatttacgg gaaccggcggcaaatatgagccagcggggattgttcaccgtggtgagttt gtcttcacgaaggaggcaaccagccggattggcgtggggaatctttaccg gctgatgcgcggctatgccaccggcggttatgtcggtacaccgggcagca tggcagacagccggtcgcaggcgtccgggacgtttgagcagaataaccat gtggtgattaacaacgacggcacgaacgggcagataggtccggctgctct gaaggcggtgtatgacatggcccgcaagggtgcccgtgatgaaattcaga cacagatgcgtgatggtggcctgttctccggaggtggacgatgaagacct tccgctggaaagtgaaacccggtatggatgtggcttcggtcccttctgta agaaaggtgcgctttggtgatggctattctcagcgagcgcctgccgggct gaatgccaacctgaaaacgtacagcgtgacgctttctgtcccccgtgagg aggccacggtactggagtcgtttctggaagagcacgggggctggaaatcc tttctgtggacgccgccttatgagtggcggcagataaaggtgacctgcgc aaaatggtcgtcgcgggtcagtatgctgcgtgttgagttcagcgcagagt ttgaacaggtggtgaactgatgcaggatatccggcaggaaacactgaatg aatgcacccgtgcggagcagtcggccagcgtggtgctctgggaaatcgac ctgacagaggtcggtggagaacgttattttttctgtaatgagcagaacga aaaaggtgagccggtcacctggcaggggcgacagtatcagccgtatccca ttcaggggagcggttttgaactgaatggcaaaggcaccagtacgcgcccc acgctgacggtttctaacctgtacggtatggtcaccgggatggcggaaga tatgcagagtctggtcggcggaacggtggtccggcgtaaggtttacgccc gttttctggatgcggtgaacttcgtcaacggaaacagttacgccgatccg gagcaggaggtgatcagccgctggcgcattgagcagtgcagcgaactgag cgcggtgagtgcctcctttgtactgtccacgccgacggaaacggatggcg ctgtttttccgggacgtatcatgctggccaacacctgcacctggacctat cgcggtgacgagtgcggttatagcggtccggctgtcgcggatgaatatga ccagccaacgtccgatatcacgaaggataaatgcagcaaatgcctgagcg gttgtaagttccgcaataacgtcggcaactttggcggcttcctttccatt aacaaactttcgcagtaaatcccatgacacagacagaatcagcgattctg gcgcacgcccggcgatgtgcgccagcggagtcgtgcggcttcgtggtaag cacgccggagggggaaagatatttcccctgcgtgaatatctccggtgagc cggaggctatttccgtatgtcgccggaagactggctgcaggcagaaatgc agggtgagattgtggcgctggtccacagccaccccggtggtctgccctgg ctgagtgaggccgaccggcggctgcaggtgcagagtgatttgccgtggtg gctggtctgccgggggacgattcataagttccgctgtgtgccgcatctca ccgggcggcgctttgagcacggtgtgacggactgttacacactgttccgg gatgcttatcatctggcggggattgagatgccggactttcatcgtgagga tgactggtggcgtaacggccagaatctctatctggataatctggaggcga cggggctgtatcaggtgccgttgtcagcggcacagccgggcgatgtgctg ctgtgctgttttggttcatcagtgccgaatcacgccgcaatttactgcgg cgacggcgagctgctgcaccatattcctgaacaactgagcaaacgagaga ggtacaccgacaaatggcagcgacgcacacactccctctggcgtcaccgg gcatggcgcgcatctgcctttacggggatttacaacgatttggtcgccgc atcgaccttcgtgtgaaaacgggggctgaagccatccgggcactggccac acagctcccggcgtttcgtcagaaactgagcgacggctggtatcaggtac ggattgccgggcgggacgtcagcacgtccgggttaacggcgcagttacat gagactctgcctgatggcgctgtaattcatattgttcccagagtcgccgg ggccaagtcaggtggcgtattccagattgtcctgggggctgccgccattg ccggatcattctttaccgccggagccacccttgcagcatggggggcagcc attggggccggtggtatgaccggcatcctgttttctctcggtgccagtat ggtgctcggtggtgtggcgcagatgctggcaccgaaagccagaactcccc gtatacagacaacggataacggtaagcagaacacctatttctcctcactg gataacatggttgcccagggcaatgttctgcctgttctgtacggggaaat gcgcgtggggtcacgcgtggtttctcaggagatcagcacggcagacgaag gggacggtggtcaggttgtggtgattggtcgctgatgcaaaatgttttat gtgaaaccgcctgcgggcggttttgtcatttatggagcgtgaggaatggg taaaggaagcagtaaggggcataccccgcgcgaagcgaaggacaacctga agtccacgcagttgctgagtgtgatcgatgccatcagcgaagggccgatt gaaggtccggtggatggcttaaaaagcgtgctgctgaacagtacgccggt gctggacactgaggggaataccaacatatccggtgtcacggtggtgttcc gggctggtgagcaggagcagactccgccggagggatttgaatcctccggc tccgagacggtgctgggtacggaagtgaaatatgacacgccgatcacccg caccattacgtctgcaaacatcgaccgtctgcgctttaccttcggtgtac aggcactggtggaaaccacctcaaagggtgacaggaatccgtcggaagtc cgcctgctggttcagatacaacgtaacggtggctgggtgacggaaaaaga catcaccattaagggcaaaaccacctcgcagtatctggcctcggtggtga tgggtaacctgccgccgcgcccgtttaatatccggatgcgcaggatgacg ccggacagcaccacagaccagctgcagaacaaaacgctctggtcgtcata cactgaaatcatcgatgtgaaacagtgctacccgaacacggcactggtcg gcgtgcaggtggactcggagcagttcggcagccagcaggtgagccgtaat tatcatctgcgcgggcgtattctgcaggtgccgtcgaactataacccgca gacgcggcaatacagcggtatctgggacggaacgtttaaaccggcataca gcaacaacatggcctggtgtctgtgggatatgctgacccatccgcgctac ggcatggggaaacgtcttggtgcggcggatgtggataaatgggcgctgta tgtcatcggccagtactgcgaccagtcagtgccggacggctttggcggca cggagccgcgcatcacctgtaatgcgtacctgaccacacagcgtaaggcg tgggatgtgctcagcgatttctgctcggcgatgcgctgtatgccggtatg gaacgggcagacgctgacgttcgtgcaggaccgaccgtcggataagacgt ggacctataaccgcagtaatgtggtgatgccggatgatggcgcgccgttc cgctacagcttcagcgccctgaaggaccgccataatgccgttgaggtgaa ctggattgacccgaacaacggctgggagacggcgacagagcttgttgaag atacgcaggccattgcccgttacggtcgtaatgttacgaagatggatgcc tttggctgtaccagccgggggcaggcacaccgcgccgggctgtggctgat taaaacagaactgctggaaacgcagaccgtggatttcagcgtcggcgcag aagggcttcgccatgtaccgggcgatgttattgaaatctgcgatgatgac tatgccggtatcagcaccggtggtcgtgtgctggcggtgaacagccagac ccggacgctgacgctcgaccgtgaaatcacgctgccatcctccggtaccg cgctgataagcctggttgacggaagtggcaatccggtcagcgtggaggtt cagtccgtcaccgacggcgtgaaggtaaaagtgagccgtgttcctgacgg tgttgctgaatacagcgtatgggagctgaagctgccgacgctgcgccagc gactgttccgctgcgtgagtatccgtgagaacgacgacggcacgtatgcc atcaccgccgtgcagcatgtgccggaaaaagaggccatcgtggataacgg ggcgcactttgacggcgaacagagtggcacggtgaatggtgtcacgccgc cagcggtgcagcacctgaccgcagaagtcactgcagacagcggggaatat caggtgctggcgcgatgggacacaccgaaggtggtgaagggcgtgagttt cctgctccgtctgaccgtaacagcggacgacggcagtgagcggctggtca gcacggcccggacgacggaaaccacataccgcttcacgcaactggcgctg gggaactacaggctgacagtccgggcggtaaatgcgtgggggcagcaggg cgatccggcgtcggtatcgttccggattgccgcaccggcagcaccgtcga ggattgagctgacgccgggctattttcagataaccgccacgccgcatctt gccgtttatgacccgacggtacagtttgagttctggttctcggaaaagca gattgcggatatcagacaggttgaaaccagcacgcgttatcttggtacgg cgctgtactggatagccgccagtatcaatatcaaaccgggccatgattat tacttttatatccgcagtgtgaacaccgttggcaaatcggcattcgtgga ggccgtcggtcgggcgagcgatgatgcggaaggttacctggattttttca aaggcaagataaccgaatcccatctcggcaaggagctgctggaaaaagtc gagctgacggaggataacgccagcagactggaggagttttcgaaagagtg gaaggatgccagtgataagtggaatgccatgtgggctgtcaaaattgagc agaccaaagacggcaaacattatgtcgcgggtattggcctcagcatggag gacacggaggaaggcaaactgagccagtttctggttgccgccaatcgtat cgcatttattgacccggcaaacgggaatgaaacgccgatgtttgtggcgc agggcaaccagatattcatgaacgacgtgttcctgaagcgcctgacggcc cccaccattaccagcggcggcaatcctccggccttttccctgacaccgga cggaaagctgaccgctaaaaatgcggatatcagtggcagtgtgaatgcga actccgggacgctcagtaatgtgacgatagctgaaaactgtacgataaac ggtacgctgagggcggaaaaaatcgtcggggacattgtaaaggcggcgag cgcggcttttccgcgccagcgtgaaagcagtgtggactggccgtcaggta cccgtactgtcaccgtgaccgatgaccatccttttgatcgccagatagtg gtgcttccgctgacgtttcgcggaagtaagcgtactgtcagcggcaggac aacgtattcgatgtgttatctgaaagtactgatgaacggtgcggtgattt atgatggcgcggcgaacgaggcggtacaggtgttctcccgtattgttgac atgccagcgggtcggggaaacgtgatcctgacgttcacgcttacgtccac acggcattcggcagatattccgccgtatacgtttgccagcgatgtgcagg ttatggtgattaagaaacaggcgctgggcatcagcgtggtctgagtgtgt tacagaggttcgtccgggaacgggcgttttattataaaacagtgagaggt

gaacgatgcgtaatgtgtgtattgccgttgctgtctttgccgcacttgcg gtgacagtcactccggcccgtgcggaaggtggacatggtacgtttacggt gggctattttcaagtgaaaccgggtacattgccgtcgttgtcgggcgggg ataccggtgtgagtcatctgaaagggattaacgtgaagtaccgttatgag ctgacggacagtgtgggggtgatggcttccctggggttcgccgcgtcgaa aaagagcagcacagtgatgaccggggaggatacgtttcactatgagagcc tgcgtggacgttatgtgagcgtgatggccggaccggttttacaaatcagt aagcaggtcagtgcgtacgccatggccggagtggctcacagtcggtggtc cggcagtacaatggattaccgtaagacggaaatcactcccgggatcctct agagtcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgt ttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagcc ggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcac attaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgt gccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgt attgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgt tcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttat ccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccag caaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatag gctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggt ggcgaaacccgacaggactataaagataccaggcgtttccccctggaagc tccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtc cgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgta ggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcac gaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtct tgagtccaacccggtaagacacgacttatcgccactggcagcagccactg gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttg aagtggtggcctaactacggctacactagaaggacagtatttggtatctg cgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgat ccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcag cagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttc tacggggtctgacgctcagtggaacgaaaactcacgttaagggattttgg tcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaa tgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag ttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttc gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgg gagggcttaccatctggccccagtgctgcaatgataccgcgagacccacg ctcaccggctccagatttatcagcaataaaccagccagccggaagggccg agcgcagaagtggtcctgcaactttatccgcctccatccagtctattaat tgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaa cgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggta tggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcc cccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgt cagaagtaagttggccgcagtgttatcactcatggttatggcagcactgc ataattctcttactgtcatgccatccgtaagatgcttttctgtgactggt gagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttg ctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactt taaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaagg atcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaa ctgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaa caggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgt tgaatactcatactcttcctttttcaatattattgaagcatttatcaggg ttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaac aaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaa gaaaccattattatcatgacattaacctataaaaataggcgtatcacgag gccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacaca tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagc agacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctg gcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgc ggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgc cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg gccagtgaattcgattttaagatacattgatgagtttggacaaaccacaa ctagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctatt gctttatttgtaaccattataagctgcaataaacaagttaacaacaacaa ttgcattcattttatgtttcaggttcagggggaggtgtgggaggtttttt aaagcaagtaaaacctctacaaatgtggtatggctgattatgatcagtta tctagagtcgcggccgctttacttgtacagctcgtccatgccgagagtga tcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcg ttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcggg cagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcgg cgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcacc ttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgtt gtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagt cgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttc acctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcg ctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgct tcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtg gtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaa cttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggaca cgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggc accaccccggtgaacagctcctcgcccttgctcaccatggtggcgaccgg tggatcgatcctagcggatctgacggttcactaaaccagctctgcttata tagacctcccaccgtacacgcctaccgcccatttgcgtcaatggggcgga gttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaac tcccattgacgtcaatggggtggagacttggaaatccccgtgagtcaaac cgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaat agcgatgactaatacgtagatgtactgccaagtaggaaagtcccataagg tcatgtactgggcataatgccaggcgggccatttaccgtcattgacgtca atagggggcgtacttggcatatgatacacttgatgtactgccaagtgggc agtttaccgtaaatactccacccattgacgtcaatggaaagtccctattg gcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcg ttgggcggtcagccaggcgggccatttaccgtaagttatgtaacgcggaa ctccatatatgggctatgaactaatgaccccgtaattgattactattaaa tcactagtgaattcgattaaagcgacggcacagctcgcggaaaatatcaa agtcgttgcgcgcctcgaactgcggcggcaccacctgtttcatggcgata atgccacggttggagtggttgccgtactggtcgagatcgttacgctcaaa ctgcgtggtcgcaggcagcacgatatcggcaaagcggcaggttgaggtcc actggttatctatggcgataaccgtttccagcttgcgccagccttcaata atgcggttgatctgctgatggcgatggaatgggttagttccggcaaaaat acacattttcagcggcggcagttttaccgatttaccgttccagttgatca ctttccccggttcgaggatcgcatcgataaaacgggcaatcggaatggtg ctgctgtagcctttgtaatcactgttgtcgtgaacaggcggaatcgacgt agagccggagaaaccactcagaataacgcctttacgccccggcgtgcctg cgccgttatagtgccagccaaaaccaaagccaccacctggcaggccaatt tgccccagcatcgccgccagaaccacaatcatccacgcccactgttcacc gtgctgcatacgctgcacgcaccagccagcaataatttgcgttctgttcg ccgccatctgccgcgccagcccacgaatggtttcggcatcaatgccggtc agtttttcagcccatgcggcatctttcggctgaccgtctttctcacccag caggtacggcaggaactgctcaaaacccacacagtagttagcgaggaagt ttttgtcgtacaggttttcactgtacagcgtatacgccagcgccagttgc agcggcacatcagtttgcgggttaaccgcaatgtgcttcacatgctcgcg ccccagatactcatgggtggatgtgacaaccggatcgatgctgatgacct caatttcaccggcggtgactttcgcttttagctgcgcgtaatattcataa acatcgtgatccgggcaccaccagttcgcttgctggtttttcagcaaatc agagccccacagcacaatggttttgctgttctgcaataccagcggccagg aggtttgctgttcatacacttccattgagccaaccacgcgcggcaggatc acctgcgcagcaccggtagagtaatctccgcccgtaccaacgctattacc atgcaaggcaatagctttcgccagcatccccgaagcgttatggaacatcc ccgtcgattgccaaccactggcggtcagcaaggcactcggcccgtgagtt ttctgcacgcgttccagttcttcatagaacatgtcgagggcttcatccca gctcacgcgcacaaaacggttatcaccgcgctgggaggtatcgctgagat ggcgcttacgcagccagtccacgcgtaccatcggataacgaatacgcgcc gcgttgtgtacgtgatccggcaatccggcaatcattttcgacggatattt atccagttcgaacggttttgccgccacaaagcgaccatccttcaccgtcg

cgcggatagccccccagtgcgacccggtaagaatgccctctttcgagatg acagcctcagtcgccgcttgcgccgcagtcgcacggcgcggcgttaacaa tgacggccccagcatcccggcgacggttaagccgccgagttgtgccagaa aacgccgacgtgatgcctgaaagagatcgttattgttcattatttttctt ccttcttatcgccgtgagccttacctgcggtgtcagacgcattcatttgc agatatttcaacaaagtgcgttcttcacgtttatcgagactggtaaagcc aatcatgccgttgagcgtgccgatccaaccgttagcgtcaaagtgggcga tttccggtgcgccgtggcactggttacaggtgccgttgtacaacgaatcc gcataagcccagatcggtttgatatcgttcaccatgtcgcctttcttcat ccacgcagtggcctgcaacttgctccactcggtattggtgtcggcaacgg tggttttctccagcgtttttacctgctgctgcacatcaccacgaatcgag gcaacaaagatgcgtttacctgggaattgggtgagtacacgctgacgtcc ggcgctttccgtccagccggtaatttcaatttgcagccagtcgccgtcac gtttaaggactttcacttccgaagcaggcagcagagaaccagaggcttct ttatcgcctttcgccgcataaattggcttaatatcaatagagtacagcgt gtcaccactgtcattagcactggcgcgcagctcatcgaactgcttacgga agccgctactcatatccggtaactggtgggcaatacctttatgacagtcg atgcaggattgattatctttcgctgccaccttcatctgacgtgccgcttc aggatgctgcttcgcatgatccatcgcatcgtagttatggcaggagcggc aggttgccgagttgttttctttcattcgcgcccattcacgctcggcaagt tccgcgcgtttggcttcgaatttttcaggtgtatcaatggagtgagcaat aaaggtctggtagatatcattgctcgcttccagtttgcgcttcaccatgc ctggaatatccggcgggatatgacagtcatggcattcagctcgcacgccg gaggcgttctggaaatgcaccgactgtttatattcttcatacaccggttg catactgtggcaactgacacaaaattcggttgtgctggtgactttgatcc caacgtgtggcaatacaatcagcgcaatgccaatcacaatcccaattgcg accagcgccagtaccgaccaacgagcactgggtcggcgtagcgcgttcca gagtttccgcataatagcccctgtaaaattatggtttagtgaagcgatct taatgagcaaatatgaacagcggcactggtcaggatgaacggcttacggc agaatatgaacagatatgaacagaatgagtaaaaccctctgatgccacat cacattgttattgttgaagatgagccggttacccaggcgcgattacaatc ttacttcactcaggaggggtataccgtttccgttacagcgagcggtgccg ggctgcgggaaattatgcagaatcagccggtagatttaattctgctggat atcaacttacccgatgaaaatggcctgatgttaacccgcgccctgcgaga acgctcaacggtggggattattctggttaccggacgcagcgatcggattg accgtattgttgggctggaaatgggcgcagacgattacgtcaccaaaccg ctggaactgcgcgaactggtagtacgggtgaaaaatctgctctggcgaat cgacctcgcgcgacaagctcaaccgcacactcaggacaactgctatcgct ttgccggttattgcctgaatgtgtcgcgccatacgctggagcgggatggc gagccgattaaactgacccgcgcagagtatgaaatgttggtggcatttgt gacgaatccgggcgaaattctcagccgtgaacgtctgctacgtatgcttt ctgcgcgtcgggtggaaaaccctgacctgcgcaccgtcgatgtgttaatt cgtcgtttacgtcataaactcagcgcggatttactggtgacgcaacatgg tgaaggttatttcttagccgctgatgtgtgctgataaaaatagaccggac gaaatccccctggtgacagcgagcggcggatatgttctcggtcggcattt ttcggcgtcagaactaaaatcggtgggctgacattatcagacaccgattg cccctgtaattgcctgatggcctgctcaactgccagttccccctgccaga ccatttgatcgctggcagccataatcactcttccccgcttcagcccgcga tacacctgatgtgaaagataaaacgacaccacggtaagcggcgttttcag gttacgcccttcacccattgccgcctctgccgcaatggccgttccggcaa cgacgtcaatttctgggtggcgttccagcatctcctgcaacaggttacgc tggatttcaatatcgttatcaccaagcgcaatatcaacaatacgcaccgg gcttccggcaatggctgcgcgaaaaccctcgaccatctctttactgcccc cggcattatcgggtccgggcatcaacagcacgttcagtggtttaccgtgc gcccattgcaccaaatatcgcccaggttgatagcccatctgaaaccaggg tacaccaacgcggcttttcacctggggagcatcaatagcatttaccagtt cgatcaccggcagacttgctacctgcttttgcagatcgggaaatgaggtc gtgctactaccgagtaaaatggcctctgcgccccactgtttacactggtc gatttgtgcttgctgggtagccaactggctgtagccgcctgcctccagca cttttaaatccacaccatagcggcgagctgcctcctgcataccatagttc aacgataaccagtatgaatctttcaggctgggataaagcgcgcacagttt ccatgcgcgtttggctttaagcggcatagaggcttgcaccgtgaaatgct gcgcatcatgccagcgcaacaggttatcagccgaaaatgccggcaacatg aaaagggaaagaagtaaaaatagcagtacgcgcatgatagcctcatcaat aataaggctttatgctagatgcattccgctttgcgactcaacctttttca ccttaagtgcaccgaccgtgaatttaaccctgacccgaagactctggatg ggctttgccctgatggcgctgttaaccctgaccagtaccctggtgggatg gtacaacctgcgctttatcagccaggtggaaaaagacaacactcaggcat tgattcctaccatgaatatggcgcgccagttgagcgaagccagcgcctgg gaacttttcgccgcgcagaacctgaccagtgccgataacgaaaagatgtg gcaggcgcaggggcgaatgctcaccgcacaaagcctgaagattaatgcgt tgctgcaagcgttacgggaacaaggttttgataccaccgctattgaacaa caggagcaggagatctcccgttcattacgtcagcaaggggaactggtggg gcggcgtctgcaactacgccagcaacaacggcaactcagtcagcagatag tcgctgccgccgatgagatcgcacgcctggcgcaaggtcaggcgaataat gcgacaacttccgctggagcgacccaggccgggatttacgatttgatcga acaagatcagcgtcaggctgctgaaagtgcactcgatcggctgattgata tcgatcttgagtatgttaaccagatgaatgaactgcgccttagcgctctg cgggtgcagcaaatggtgatgaatctggggctggagcagatccagaaaaa tgcaccaacgctggaaaagcagctcaataatgcggtgaaaattctgcaac gtcggcaaatacgcattgaagatccgggtgttcgtgcgcaggtcgcaaca acgttaactaccgttagccaatatagcgatttgctggcgctgtatcagca ggacagtgaaatcagcaatcacctacaaactctcgcacaaaataacatcg cccagttcgcgcagtttagtagcgaagtcagtcagctggtcgactcggta cccggggatccactcgttattctcggacgagtgttcagtaatgaacctct ggagagaaccatgtatatgatcgttatctgggttggacttctgcttttaa gcccagataactggcctgaatatgttaatgagagaatcggtattcctcat gtgtggcatgttttcgtctttgctcttgcattttcgctagcaattaatgt gcatcgattatcagctattgccagcgccagatataagcgatttaagctaa gaaaacgcattaagatgcaaaacgataaagtgcgatcagtaattcaaaac cttacagaagagcaatctatggttttgtgcgcagcccttaatgaaggcag gaagtatgtggttacatcaaaacaattcccatacattagtgagttgattg agcttggtgtgttgaacaaaactttttcccgatggaatggaaagcatata ttattccctattgaggatatttactggactgaattagttgccagctatga tccatataatattgagataaagccaaggccaatatctaagtaactagata agaggaatcgattttcccttaattttctggcgtccactgcatgttatgcc gcgttcgccaggcttgctgtaccatgtgcgctgattcttgcgctcaatac gttgcaggttgctttcaatctgtttgtggtattcagccagcactgtaagg tctatcggatttagtgcgctttctactcgtgatttcggtttgcgattcag cgagagaatagggcggttaactggttttgcgcttaccccaaccaacaggg gatttgctgctttccattgagcctgtttctctgcgcgacgttcgcggcgg cgtgtttgtgcatccatctggattctcctgtcagttagctttggtggtgt gtggcagttgtagtcctgaacgaaaaccccccgcgattggcacattggca gctaatccggaatcgcacttacggccaatgcttcgtttcgtatcacacac cccaaagccttctgctttgaatgctgcccttcttcagggcttaattttta agagcgtcaccttcatggtggtcagtgcgtcctgctgatgtgctcagtat caccgccagtggtatttatgtcaacaccgccagagataatttatcaccgc agatggttatctgtatgttttttatatgaatttattttttgcaggggggc attgtttggtaggtgagagatctgaattgctatgtttagtgagttgtatc tatttatttttcaataaatacaattggttatgtgttttgggggcgatcgt gaggcaaagaaaacccggcgctgaggccgggttattcttgttctctggtc aaattatatagttggaaaacaaggatgcatatatgaatgaacgatgcaga ggcaatgccgatggcgatagtgggtatcatgtagccgcttatgctggaaa gaagcaataacccgcagaaaaacaaagctccaagctcaacaaaactaagg gcatagacaataactaccgatgtcatatacccatactctctaatcttggc cagtcggcgcgttctgcttccgattagaaacgtcaaggcagcaatcagga ttgcaatcatggttcctgcatatgatgacaatgtcgccccaagaccatct ctatgagctgaaaaagaaacaccaggaatgtagtggcggaaaaggagata gcaaatgcttacgataacgtaaggaattattactatgtaaacaccaggca tgattctgttccgcataattactcctgataattaatccttaactttgccc acctgccttttaaaacattccagtatatcacttttcattcttgcgtagca atatgccatctcttcagctatctcagcattggtgaccttgttcagaggcg ctgagagatggcctttttctgatagataatgttctgttaaaatatctccg gcctcatcttttgcccgcaggctaatgtctgaaaattgaggtgacgggtt aaaaataatatccttggcaaccttttttatatcccttttaaattttggct taatgactatatccaatgagtcaaaaagctccccttcaatatctgttgcc

cctaagacctttaatatatcgccaaatacaggtagcttggcttctacctt caccgttgttcggccgatgaaatgcatatgcataacatcgtctttggtgg ttcccctcatcagtggctctatctgaacgcgctctccactgcttaatgac attcctttcccgattaaaaaatctgtcagatcggatgtggtcggcccgaa aacagttctggcaaaaccaatggtgtcgccttcaacaaacaaaaaagatg ggaatcccaatgattcgtcatctgcgaggctgttcttaatatcttcaact gaagctttagagcgatttatcttctgaaccagactcttgtcatttgtttt ggtaaagagaaaagtttttccatcgattttatgaatatacaaataattgg agccaacctgcaggtgatgattatcagccagcagagaattaaggaaaaca gacaggtttattgagcgcttatctttccctttatttttgctgcggtaagt cgcataaaaaccattcttcataattcaatccatttactatgttatgttct gaggggagtgaaaattcccctaattcgatgaagattcttgctcaattgtt atcagctatgcgccgaccagaacaccttgccgatcagccaaacgtctctt caggccactgactagcgataactttccccacaacggaacaactctcattg catgggatcattgggtactgtgggtttagtggttgtaaaaacacctgacc gctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctgg ctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaatt aacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatgga attaccttcaacctcaagccagaatgcagaatcactggcttttttggttg tgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggt gagaacatccctgcctgaacatgagaaaaaacagggtactcatactcact tctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctc tggcgattgaagggctaaattcttcaacgctaactttgagaatttttgca agcaatgcggcgttataagcatttaatgcattgatgccattaaataaagc accaacgcctgactgccccatccccatcttgtctgcgacagattcctggg ataagccaagttcatttttctttttttcataaattgctttaaggcgacgt gcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatacg ttaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgact attttacctctggcggtgataatggttgcatgtactaaggaggttgtatg gaacaacgcataaccctgaaagattatgcaatgcgctttgggcaaaccaa gacagctaaagatctcggcgtatatcaaagcgcgatcaacaaggccattc atgcaggccgaaagatttttttaactataaacgctgatggaagcgtttat gcggaagaggtaaagcccttcccgagtaacaaaaaaacaacagcataaat aaccccgctcttacacattccagccctgaaaaagggcatcaaattaaacc acacctatggtgtatgcatttatttgcatacattcaatcaattgttatct aaggaaatacttacatatggttcgtgcaaacaaacgcaacgaggctctac gaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaag acagcggaagctgtgggcgttgataagtcgcagatcagcaggtggaagag ggactggattccaaagttctcaatgctgcttgctgttcttgaatgggggg tcgttgacgacgacatggctcgattggcgcgacaagttgctgcgattctc accaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagat ggagttctgaggtcattactggatctatcaacaggagtcattatgacaaa tacagcaaaaatactcaacttcggcagaggtaactttgccggacaggagc gtaatgtggcagatctcgatgatggttacgccagactatcaaatatgctg cttgaggcttattcgggcgcagatctgaccaagcgacagtttaaagtgct gcttgccattctgcgtaaaacctatgggtggaataaaccaatggacagaa tcaccgattctcaacttagcgagattacaaagttacctgtcaaacggtgc aatgaagccaagttagaactcgtcagaatgaatattatcaagcagcaagg cggcatgtttggaccaaataaaaacatctcagaatggtgcatccctcaaa acgagggaaaatcccctaaaacgagggataaaacatccctcaaattgggg gattgctatccctcaaaacagggggacacaaaagacactattacaaaaga aaaaagaaaagattattcgtcagannunnntggcgaatcctctgaccagc cagaaaacgacctttctgtggtgaaaccggatgctgcaattcagagcggc agcaagtgggggacagcagaagacctgaccgccgcagagtggatgtttga catggtgaagactatcgcaccatcagccagaaaaccgaattttgctgggt gggctaacgatatccgcctgatgcgtgaacgtgacggacgtaaccaccgc gacatgtgtgtgctgttccgctgggcatgccaggacaacttctggtccgg taacgtgctgagcccggccaaactccgcgataagtggacccaactcgaaa tcaaccgtaacaagcaacaggcaggcgtgacagccagcaaaccaaaactc gacctgacaaacacagactggatttacggggtggatctatgaaaaacatc gccgcacagatggttaactttgaccgtgagcagatgcgtcggatcgccaa caacatgccggaacagtacgacgaaaagccgcaggtacagcaggtagcgc agatcatcaacggtgtgttcagccagttactggcaactttcccggcgagc ctggctaaccgtgaccagaacgaagtgaacgaaatccgtcgccagtgggt tctggcttttcgggaaaacgggatcaccacgatggaacaggttaacgcag gaatgcgcgtagcccgtcggcagaatcgaccatttctgccatcacccggg cagtttgttgcatggtgccgggaagaagcatccgttaccgccggactgcc aaacgtcagcgagctggttgatatggtttacgagtattgccggaagcgag gcctgtatccggatgcggagtcttatccgtggaaatcaaacgcgcactac tggctggttaccaacctgtatcagaacatgcgggccaatgcgcttactga tgcggaattacgccgtaaggccgcagatgagcttgtccatatgactgcga gaattaaccgtggtgaggcgatccctgaaccagtaaaacaacttcctgtc atgggcggtagacctctaaatcgtgcacaggctctggcgaagatcgcaga aatcaaagctaagttcggactgaaaggagcaagtgtatgacgggcaaaga ggcaattattcattacctggggacgcataatagcttctgtgcgccggacg ttgccgcgctaacaggcgcaacagtaaccagcataaatcaggccgcggct aaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaac ggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcg atgaacaaactggatacgattggattcgacaacaaaaaagacctgcttat ctcggtgggcgatttggttgatcgtggtgcagagaacgttgaatgcctgg aattaatcacattcccctggttcagagctgtacgtggaaaccatgagcaa atgatgattgatggcttatcagagcgtggaaacgttaatcactggctgct taatggcggtggctggttctttaatctcgattacgacaaagaaattctgg ctaaagctcttgcccataaagcagatgaacttccgttaatcatcgaactg gtgagcaaagataaaaaatatgttatctgccacgccgattatccctttga cgaatacgagtttggaaagccagttgatcatcagcaggtaatctggaacc gcgaacgaatcagcaactcacaaaacgggatcgtgaaagaaatcaaaggc gcggacacgttcatctttggtcatacgccagcagtgaaaccactcaagtt tgccaaccaaatgtatatcgataccggcgcagtgttctgcggaaacctaa cattgattcaggtacagggagaaggcgcatgagactcgaaagcgtagcta aatttcattcgccaaaaagcccgatgatgagcgactcaccacgggccacg gcttctgactctctttccggtactgatgtgatggctgctatggggatggc gcaatcacaagccggattcggtatggctgcattctgcggtaagcacgaac tcagccagaacgacaaacaaaaggctatcaactatctgatgcaatttgca cacaaggtatcggggaaataccgtggtgtggcaaagcttgaaggaaatac taaggcaaaggtactgcaagtgctcgcaacattcgcttatgcggattatt gccgtagtgccgcgacgccgggggcaagatgcagagattgccatggtaca ggccgtgcggttgatattgccaaaacagagctgtgggggagagttgtcga gaaagagtgcggaagatgcaaaggcgtcggctattcaaggatgccagcaa gcgcagcatatcgcgctgtgacgatgctaatcccaaaccttacccaaccc acctggtcacgcactgttaagccgctgtatgacgctctggtggtgcaatg ccacaaagaagagtcaatcgcagacaacattttgaatgcggtcacacgtt agcagcatgattgccacggatggcaacatattaacggcatgatattgact tattgaataaaattgggtaaatttgactcaacgatgggttaattcgctcg ttgtggtagtgagatgaaaagaggcggcgcttactaccgattccgcctag ttggtcacttcgacgtatcgtctggaactccaaccatcgcaggcagagag gtctgcaaaatgcaatcccgaaacagttcgcaggtaatagttagagcctg cataacggtttcgggattttttatatctgcacaacaggtaagagcattga gtcgataatcgtgaagagtcggcgagcctggttagccagtgctctttccg ttgtgctgaattaagcgaataccggaagcagaaccggatcaccaaatgcg tacaggcgtcatcgccgcccagcaacagcacaacccaaactgagccgtag ccactgtctgtcctnnnnnnattagtaatagttacgctgcggccttttac acatgaccttcgtgaaagcgggtggcaggaggtcgcgctaacaacctcct gccgttttgcccgtgcatatcggtcacgaacaaatctgattactaaacac agtagcctggatttgttctatcagtaatcgaccttattcctaattaaata gagcaaatccccttattgggggtaagacatgaagatgccagaaaaacatg acctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatc cttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtt tacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttca ttcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatata acgagcgtgtttatcggctacatcggtactgactcgattggttcgcttat caaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaat aatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactga taacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcg gagagctatttactgattactccgatcaccctcgcaaacttgtcacgcta

aacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccg ttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccga aaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgcttta cctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaa tatctgggcttcactgccgggcgctggttatggtcagttcgagcataagg ctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagag attgatgtatgagcagagtcaccgcgattatctccgctctggttatctgc atcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccat tacctacaaagcccagcgcgacaaaaatgccagagaactgaagctggcga acgcggcaattactgacatgcagatgcgtcagcgtgatgttgctgcgctc gatgcaaaatacacgaaggagttagctgatgctaaagctgaaaatgatgc tctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcag tctgtcagtcagtgcgtgaagccaccaccgcctccggcgtggataatgca gcctccccccgactggcagacaccgctgaacgggattatttcaccctcag agagaggctgatcactatgcaaaaacaactggaaggaacccagaagtata ttaatgagcagtgcagatagagttgcccatatcgatgggcaactcatgca attattgtgagcaatacacacgcgcttccagcggagtataaatgcctaaa gtaataaaaccgagcaatccatttacgaatgtttgctgggtttctgtttt aacaacattttctgcgccgccacaaattttggctgcatcgacagttttct tctgcccaattccagaaacgaagaaatgatgggtgatggtttcctttggt gctactgctgccggtttgttttgaacagtaaacgtctgttgagcacatcc tgtaataagcagggccagcgcagtagcgagtagcatttttttcatggtgt tattcccgatgctttttgaagttcgcagaatcgtatgtgtagaaaattaa acaaaccctaaacaatgagttgaaatttcatattgttaatatttattaat gtatgtcaggtgcgatgaatcgtcattgtattcccggattaactatgtcc acagccctgacggggaacttctctgcgggagtgtccgggaataattaaaa cgatgcacacagggtttagcgcgtacacgtattgcattatgccaacgccc cggtgctgacacggaagaaaccggacgttatgatttagcgtggaaagatt tgtgtagtgttctgaatgctctcagtaaatagtaatgaattatcaaaggt atagtaatatcttttatgttcatggatatttgtaacccatcggaaaactc ctgctttagcaagattttccctgtattgctgaaatgtgatttctcttgat ttcaacctatcataggacgtttctataagatgcgtgtttcttgagaattt aacatttacaacctttttaagtccttttattaacacggtgttatcgtttt ctaacacgatgtgaatattatctgtggctagatagtaaatataatgtgag acgttgtgacgttttagttcagaataaaacaattcacagtctaaatcttt tcgcacttgatcgaatatttctttaaaaatggcaacctgagccattggta aaaccttccatgtgatacgagggcgcgtagtttgcattatcgtttttatc gtttcaatctggtctgacctccttgtgttttgttgatgatttatgtcaaa tattaggaatgttttcacttaatagtattggttgcgtaacaaagtgcggt cctgctggcattctggagggaaatacaaccgacagatgtatgtaaggcca acgtgctcaaatcttcatacagaaagatttgaagtaatattttaaccgct agatgaagagcaagcgcatggagcgacaaaatgaataaagaacaatctgc tgatgatccctccgtggatctgattcgtgtaaaaaatatgcttaatagca ccatttctatgagttaccctgatgttgtaattgcatgtatagaacataag gtgtctctggaagcattcagagcaattgaggcagcgttggtgaagcacga taataatatgaaggattattccctggtggttgactgatcaccataactgc taatcattcaaactatttagtctgtgacagagccaacacgcagtctgtca ctgtcaggaaagtggtaaaactgcaactcaattactgcaatgccctcgta attaagtgaatttacaatatcgtcctgttcggagggaagaacgcgggatg ttcattcttcatcacttttaattgatgtatatgctctcttttctgacgtt agtctccgacggcaggcttcaatgacccaggctgagaaattcccggaccc tttttgctcaagagcgatgttaatttgttcaatcatttggttaggaaagc ggatgttgcgggttgttgttctgcgggttctgttcttcgttgacatgagg ttgccccgtattcagtgtcgctgatttgtattgtctgaagttgtttttac gttaagttgatgcagatcaattaatacgatacctgcgtcataattgatta tttgacgtggtttgatggcctccacgcacgttgtgatatgtagatgataa tcattatcactttacgggtcctttccggtgatccgacaggttacg

TABLE-US-00006 TABLE 6 Sequence of pTn-I-Sce2-PsapLuxAB-Cm1. tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccg gagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccg tcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatg cggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaata ccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccatt caggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctat tacgacagctgtctcttatacacatctcaaccatcatcgatgaattttct cgggtagggataacagggtaatgaattgaaattacgccccgccctgccac tcatcgcagtactgttgtaattcattaagcattctgccgacatggaagcc atcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgt cgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttg tccatattggccacgtttaaatcaaaactggtgaaactcacccagggatt ggctgagacgaaaaacatattctcaataaaccattagggaaataggccag gttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgcc ggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgc tcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctc accgtctttcattgccatacggaattccggatgagcattcatcaggcggg caagaatgtgaataaaggccggataaaacttgtgcttatttttctttacg gtctttaaaaaggccgtaatatccagctgaacggtctggttataggtaca ttgagcaactgactgaaatgcctcaaaatgttctttacgatgccattggg atatatcaacggtggtatatccagtgatttttttctccattttagcttcc ttagctccagatctatggatcgctcgaaaaaatcaaatcatcaatttctg agcttgctgtaatttcttgtttatcaacatatgaagtcatacctgtttta ctcctcaagataatattagaaagtatggcagcactgctgtcatactcttt tatacccttcatctttcaagctgctgctttgttggctgctttcactcacc ccagtcacatagttatctatgctcctggggattcgttcacttgccgcctc gctgcaactcgaaatctattaggtatattccatgtggtacttataatatt atcatcaacaatattgattacatttttttggctcatcaaatcattcattg gttcaaaggacagcaatacacttttcgcaccacacttttcaattgccaac ttagccgcagttatacactccgtataatttccgacagcgttttctgcaat tatttcttcaagtttattttcgaaattttcattagggtgcatttcaagaa cataatcactaataaatgcacgcgtctcttgtttagctttattactatct tcgttatagttaactaatatcattaactgatggtctatctctgataggtc aacgtcatatttatccgcaacggctttatatctttcagcatattcatatc taacatcattagaatcatcccacttaaagatgagaggaataccttttttg gccgcccactcaacaatatgatgactggttgctgttacatatttccgagg tccgcctggcgtataagcatggggatttacagatattttagggaagctat aaaaatcgttatctggattacaatagcctgttgttaaagcatcgttaatg atttcataacactcttcaaatagttgctgttgatattcaaccgggcgatt aaaaaaatgcatttcatcttttttttcgcaatcactaaaccctaaaataa atctcccttcacttaactgatccaataagcaagcttcctccgctatggcg acaggatgatgagttgtaatgatgtgatttaatgaaccaattttaatttt ctctgttaaaccgagcagaaaaccagaaacagtcagaggagcgccgacaa caccattatctgaaaaatgattttcatacactaaaatctgttcaaaattc aacttatcaacatactccgttatttcctgcatgcgaactatactttgttc ttgaacagttgttgaattgatgaagttaaggaagaacaatccaaatttca tttctttctccttagctaatataatagcgaacgttgtttttctttaagaa atggcatgacatcagactggaagagcttcatggaagcaataatttcgtct actgttccattagcttcaaatccacaacaaatatttgatattcctgtagc atcaatgtctttttgaattatgtcaatacattcctgcggcgttcccacgg gattgatttcgtaactgtaatcaatacggcgattagtatctttatgtcct tttaatacaaagtcacgccactgccctttattgaaatcataacctcttgt ttggtctgaatcatcaaaaatagtcgtagcattcacataagaatcatacc aatgccccagaaatttccggcaaatctctttcgctttaattgagtcatga tctacagatgttatatatgataagcaatggtcgatattatgaatatcgtg cccatattcttgagccacttcattataaagctcaagttgtgctttctttt cgttagtatttataatccaacttaatatcatcggtaggccaaattgagca gcccactcagtcgtcgaagctgattcagccaccacataaaccggtgcgcc acctctgctatacgccgcggggtttacttttaccttatggaacttgatat gttcattatcagcttccatatatccctctgtcatgccattctttatcagc ccgtaccagcattccgctaaggcgcgactgttattcatatctgtgccgaa tacgcgaaagtccttgttgtaaagccctcggcaaataccaaaccgaaatc gtccttttgacatttgatccaataaattcacatcttcaagttggcgtact ggatgggctgtgggaagaacaatagcggcagttcctacattcaatttttt agtcgcgccaagtaaatatgcagcagcgacataagggttaccaagcaaac caaactccgtgaaatgatgctccagtaaccatacggtatcaaaaccacac tcctcagagatgcgacctaatttaaccaaacgtttcattacctctgtttg agaaaattggggaggttggtatgtaagcaaaaagtttccaaatttcatag agagtccttatattgctatttgagtgatagaatatctcaatagattttaa gacagagaaattgcttgattttcaatctcaattctcattcggcgttcatt gactgtcgcaatagttaaatgttcaaatgacggttcagtaatatcaacat caatatccagatgatcattatccatcgtactgcgcgaattataacataat tttttataaaattcaaatttatgcaggcatgcaagtcgacattaccctgt tatccctactgcaggcatgcaagcttgccaacgactacgcactagccaac aagagcttcagggttgagatgtgtataagagacagctgtcttaatgaatc ggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttc ctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtat cagctcactcaaaggcggtaatacggttatccacagaatcaggggataac gcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaa aaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagc atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggacta taaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgt tccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaa gcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtag gtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccga ccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagac acgacttatcgccactggcagcagccactggtaacaggattagcagagcg aggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacgg ctacactagaaggacagtatttggtatctgcgctctgctgaagccagtta ccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgct ggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaa aggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaagg atcttcacctagatccttttaaattaaaaatgaagttttaaatcaatcta aagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtg aggcacctatctcagcgatctgtctatttcgttcatccatagttgcctga ctccccgtcgtgtagataactacgatacgggagggcttaccatctggccc cagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgca actttatccgcctccatccagtctattaattgttgccgggaagctagagt aagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacag gcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggt tcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcag tgttatcactcatggttatggcagcactgcataattctcttactgtcatg ccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcatt ctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatac gggataataccgcgccacatagcagaactttaaaagtgctcatcattgga aaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatc cagttcgatgtaacccactcgtgcacccaactgatcttcagcatctttta ctttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgca aaaaagggaataagggcgacacggaaatgttgaatactcatactcttcct ttttcaatattattgaagcatttatcagggttattgtctcatgagcggat acatatttgaatgtatttagaaaaataaacaaataggggttccgcgcaca tttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgac attaacctataaaaataggcgtatcacgaggccctttcgtc

TABLE-US-00007 TABLE 7 Sequence of M13LuxABE2 (SEQ ID NO: 1). Sequences encoding luciferase subunits are shown in underline. aatgctactactattagtagaattgatgccaccttttcagctcgcgcccc aaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatcta atggtcaaactaaatctactcgttcgcagaattgggaatcaactgttaca tggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgt tgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaa tgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctg ttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcg atatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgct ttgcttctgactataatagtcagggtaaagacctgatttttgatttatgg tcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaa tatttatgacgattccgcagtattggacgctatccagtctaaacatttta ctattaccccctctggcaaaacttcttttgcaaaagcctctcgctatttt ggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttac tatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtg gtattcctaaatctcaactgatgaatctttctacctgtaataatgttgtt ccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactg gtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaa agttgaaattaaaccatctcaagcccaatttactactcgttctggtgttt ctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgat ttgggtaatgaatatccggttcttgtcaagattactcttgatgaaggtca gccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaag ttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggct aagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatga tacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggt caaagatgagtgttttagtgtattctttcgcctctttcgttttaggttgg tgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctc atgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgt tccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcct ttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcg atggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaa attcacctcgaaagcaagctgataaaccgatacaattaaaggctcctttt ggagcctttttttttggagattttcaacgtgaaaaaattattattcgcaa ttcctttagttgttcctttctattctcactccgctgaaactgttgaaagt tgtttagcaaaaccccatacagaaaattcatttactaacgtctggaaaga cgacaaaactttagatcgttacgctaactatgagggttgtctgtggaatg ctacaggcgttgtagtttgtactggtgacgaaactcagtgttacggtaca tgggttcctattgggcttgctatccctgaaaatgagggtggtggctctga gggtggcggttctgagggtggcggttctgagggtggcggtactaaacctc ctgagtacggtgatacacctattccgggctatacttatatcaaccctctc gacggcacttatccgcctggtactgagcaaaaccccgctaatcctaatcc ttctcttgaggagtctcagcctcttaatactttcatgtttcagaataata ggttccgaaataggcagggggcattaactgtttatacgggcactgttact caaggcactgaccccgttaaaacttattaccagtacactcctgtatcatc aaaagccatgtatgacgcttactggaacggtaaattcagagactgcgctt tccattctggctttaatgaagatccattcgtttgtgaatatcaaggccaa tcgtctgacctgcctcaacctcctgtcaatgctggcggcggctctggtgg tggttctggtggcggctctgagggtggtggctctgagggtggcggttctg agggtggcggctctgagggaggcggttccggtggtggctctggttccggt gattttgattatgaaaagatggcaaacgctaataagggggctatgaccga aaatgccgatgaaaacgcgctacagtctgacgctaaaggcaaacttgatt ctgtcgctactgattacggtgctgctatcgatggtttcattggtgacgtt tccggccttgctaatggtaatggtgctactggtgattttgctggctctaa ttcccaaatggctcaagtcggtgacggtgataattcacctttaatgaata atttccgtcaatatttaccttccctccctcaatcggttgaatgtcgccct tttgtctttagcgctggtaaaccatatgaattttctattgattgtgacaa aataaacttattccgtggtgtctttgcgtttcttttatatgttgccacct ttatgtatgtattttctacgtttgctaacatactgcgtaataaggagtct taatcatgccagttcttttgggtattccgttattattgcgtttcctcggt ttccttctggtaactttgttcggctatctgcttacttttcttaaaaaggg cttcggtaagatagctattgctatttcattgtttcttgctcttattattg ggcttaactcaattcttgtgggttatctctctgatattagcgctcaatta ccctctgactttgttcagggtgttcagttaattctcccgtctaatgcgct tccctgtttttatgttattctctctgtaaaggctgctattttcatttttg acgttaaacaaaaaatcgtttcttatttggattgggataaataatatggc tgtttattttgtaactggcaaattaggctctggaaagacgctcgttagcg ttggtaagattcaggataaaattgtagctgggtgcaaaatagcaactaat cttgatttaaggcttcaaaacctcccgcaagtcgggaggttcgctaaaac gcctcgcgttcttagaataccggataagccttctatatctgatttgcttg ctattgggcgcggtaatgattcctacgatgaaaataaaaacggcttgctt gttctcgatgagtgcggtacttggtttaatacccgttcttggaatgataa ggaaagacagccgattattgattggtttctacatgctcgtaaattaggat gggatattatttttcttgttcaggacttatctattgttgataaacaggcg cgttctgcattagctgaacatgttgtttattgtcgtcgtctggacagaat tactttaccttttgtcggtactttatattctcttattactggctcgaaaa tgcctctgcctaaattacatgttggcgttgttaaatatggcgattctcaa ttaagccctactgttgagcgttggctttatactggtaagaatttgtataa cgcatatgatactaaacaggctttttctagtaattatgattccggtgttt attcttatttaacgccttatttatcacacggtcggtatttcaaaccatta aatttaggtcagaagatgaaattaactaaaatatatttgaaaaagttttc tcgcgttctttgtcttgcgattggatttgcatcagcatttacatatagtt atataacccaacctaagccggaggttaaaaaggtagtctctcagacctat gattttgataaattcactattgactcttctcagcgtcttaatctaagcta tcgctatgttttcaaggattctaagggaaaattaattaatagcgacgatt tacagaagcaaggttattcactcacatatattgatttatgtactgtttcc attaaaaaaggtaattcaaatgaaattgttaaatgtaattaattttgttt tcttgatgtttgtttcatcatcttcttttgctcaggtaattgaaatgaat aattcgcctctgcgcgattttgtaacttggtattcaaagcaatcaggcga atccgttattgtttctcccgatgtaaaaggtactgttactgtatattcat ctgacgttaaacctgaaaatctacgcaatttctttatttctgttttacgt gctaataattttgatatggttggttcaattccttccataattcagaagta taatccaaacaatcaggattatattgatgaattgccatcatctgataatc aggaatatgatgataattccgctccttctggtggtttctttgttccgcaa aatgataatgttactcaaacttttaaaattaataacgttcgggcaaagga tttaatacgagttgtcgaattgtttgtaaagtctaatacttctaaatcct caaatgtattatctattgacggctctaatctattagttgttagtgcacct aaagatattttagataaccttcctcaattcctttctactgttgatttgcc aactgaccagatattgattgagggtttgatatttgaggttcagcaaggtg atgctttagatttttcatttgctgctggctctcagcgtggcactgttgca ggcggtgttaatactgaccgcctcacctctgttttatcttctgctggtgg ttcgttcggtatttttaatggcgatgttttagggctatcagttcgcgcat taaagactaatagccattcaaaaatattgtctgtgccacgtattcttacg ctttcaggtcagaagggttctatctctgttggccagaatgtcccttttat tactggtcgtgtgactggtgaatctgccaatgtaaataatccatttcaga cgattgagcgtcaaaatgtaggtatttccatgagcgtttttcctgttgca atggctggcggtaatattgttctggatattaccagcaaggccgatagttt gagttcttctactcaggcaagtgatgttattactaatcaaagaagtattg ctacaacggttaatttgcgtgatggacagactcttttactcggtggcctc actgattataaaaacacttctcaagattctggcgtaccgttcctgtctaa aatccctttaatcggcctcctgtttagctcccgctctgattccaacgagg aaagcacgttatacgtgctcgtcaaagcaaccatagtacgcgccctgtag cggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgcta cacttgccagcgccctagcgcccgctcctttcgctttcttcccttccttt ctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccc tttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttg atttgggtgatggttcacgtagtgggccatcgccctgatagacggttttt cgccctttgacgttggagtccacgttctttaatagtggactcttgttcca aactggaacaacactcaaccctatctcgggctattcttttgatttataag ggattttgccgatttcggaaccaccatcaaacaggattttcgcctgctgg ggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtg aagggcaatcagctgttgcccgtctcgctggtgaaaagaaaaaccaccct ggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa tgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa

cgcaattaatgtgagttagctcactcattaggcaccccaggctttacact ttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaattt cacacaggaaacagctatgaccatgattacgaattcactagtgattatgg taaagcaagatgaagttatcacattgttatcaaatattcgtagtaatcga tgcaagatatattctttgttaggaagttcgcatgacttgagtgaaaattt agtggtcctgcgcaatttttatcaatcggttacgaaagccgctatcgcga tggataatgatcatctggatattgatgttgatattactgaaccgtcattt gaacatttaactattgcgacagtcaatgaacgccgaatgagaattgagat tgaaaatcaagcaatttctctgtcttaaaatctattgagatattctatca ctcaaatagcaatataaggactctctatgaaatttggaaactttttgctt acataccaacctccccaattttctcaaacagaggtaatgaaacgtttggt taaattaggtcgcatctctgaggagtgtggttttgataccgtatggttac tggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgct gctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgc tattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatt tattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgaggg ctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcg cgccttagcggaatgctggtacgggctgataaagaatggcatgacagagg gatatatggaagctgataatgaacatatcaagttccataaggtaaaagta aaccccgcggcgtatagcagaggtggcgcaccggtttatgtggtggctga atcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatat taagttggattataaatactaacgaaaagaaagcacaacttgagctttat aatgaagtggctcaagaatatgggcacgatattcataatatcgaccattg cttatcatatataacatctgtagatcatgactcaattaaagcgaaagaga tttgccggaaatttctggggcattggtatgattcttatgtgaatgctacg actatttttgatgattcagaccaaacaagaggttatgatttcaataaagg gcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgta ttgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtatt gacataattcaaaaagacattgatgctacaggaatatcaaatatttgttg tggatttgaagctaatggaacagtagacgaaattattgcttccatgaagc tcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgcta ttatattagctaaggagaaagaaatgaaatttggattgttcttccttaac ttcatcaattcaacaactgttcaagaacaaagtatagttcgcatgcagga aataacggagtatgttgataagttgaattttgaacagattttagtgtatg aaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttct ggttttctgctcggtttaacagagaaaattaaaattggttcattaaatca catcattacaactcatcatcctgtcgccatagcggaggaagcttgcttat tggatcagttaagtgaagggagatttattttagggtttagtgattgcgaa aaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagca actatttgaagagtgttatgaaatcattaacgatgctttaacaacaggct attgtaatccagataacgatttttatagcttccctaaaatatctgtaaat ccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccag tcatcatattgttgagtgggcggccaaaaaaggtattcctctcatcttta agtgggatgattctaatgatgttagatatgaatatgctgaaagatataaa gccgttgcggataaatatgacgttgacctatcagagatagaccatcagtt aatgatattagttaactataacgaagatagtaataaagctaaacaagaga cgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaat ttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaatta tacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcga aaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaa aatgtaatcaatattgttgatgataatattaagaagtaccacatggaata tacctaatagatttcgagttgcagcgaggcggcaagtgaacgaatcccca ggagcatagataactatgtgactggggtgagtgaaagcagccaacaaagc agcagcttgaaagatgaagggtataaaagagtatgacagcagtgctgcca tactttctaatattatcttgaggagtaaaacaggtatgacttcatatgtt gataaacaagaaattacagcaagctcagaaattgatgatttgattttttc gagcgatccattagtgtggtcttacgacgagcaggaaaaaatcagaaaga aacttgtgcttgatgcatttcgtaatcattataaacattgtcgagaatat cgtcactactgtcaggcacacaaagtagatgacaatattacggaaattga tgacatacctgtattcccaacatcggtttttaagtttactcgcttattaa cttctcaggaaaacgagattgaaagttggtttaccagtagcggcacgaat ggtttaaaaagtcaggtggcgcgtgacagattaagtattgagagactctt aggctctgtgagttatggcatgaaatatgttggtagttggtttgatcatc aaatagaattagtcaatttgggaccagatagatttaatgctcataatatt tggtttaaatatgttatgagtttggtggaattgttatatcctacgacatt taccgtaacagaagaacgaatagattttgttaaaacattgaatagtcttg aacgaataaaaaatcaagggaaagatctttgtcttattggttcgccatac tttatttatttactctgccattatatgaaagataaaaaaatctcattttc tggagataaaagcctttatatcataaccggaggcggctggaaaagttacg aaaaagaatctctgaaacgtgatgatttcaatcatcttttatttgatact ttcaatctcagtgatattagtcagatccgagatatatttaatcaagttga actcaacacttgtttctttgaggatgaaatgcagcgtaaacatgttccgc cgtgggtatatgcgcgagcgcttgatcctgaaacgttgaaacctgtacct gatggaacgccggggttgatgagttatatggatgcgtcagcaaccagtta tccagcatttattgttaccgatgatgtcgggataattagcagagaatatg gtaagtatcccggcgtgctcgttgaaattttacgtcgcgtcaatacgagg acgcagaaagggtgtgctttaagcttaaaccaagcatttaatagttgaca taatcgaattcccgggagagctcgatatcgcatgcggtacctctagaaga agcttgggatccgtcgacctgcagcaattcactggccgtcgttttacaac gtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagca catccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcg cccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggt ttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcct gaggccgatacggtcgtcgtcccctcaaactggcagatgcacggttacga tgcgcccatctacaccaacgtaacctatcccattacggtcaatccgccgt ttgttcccacggagaatccgacgggttgttactcgctcacatttaatgtt gatgaaagctggctacaggaaggccagacgcgaattatttttgatggcgt tcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattt taacaaaatattaacgtttacaatttaaatatttgcttatacaatcttcc tgtttttggggcttttctgattatcaaccggggtacatatgattgacatg ctagttttacgattaccgttcatcgattctcttgtttgctccagactctc aggcaatgacctgatagcctttgtagatctctcaaaaatagctaccctct ccggcattaatttatcagctagaacggttgaatatcatattgatggtgat ttgactgtctccggcctttctcacccttttgaatctttacctacacatta ctcaggcattgcatttaaaatatatgagggttctaaaaatttttatcctt gcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtt tttggtacaaccgatttagctttatgctctgaggctttattgcttaattt tgctaattctttgccttgcctgtatgatttattggatgtt

TABLE-US-00008 TABLE 8 Sequence of T71-2aLuxAB (SEQ ID NO: 2). Sequences encoding luciferase subunits are shown in underline. tctcacagtgtacggacctaaagttcccccatagggggtacctaaagcccagccaatcacctaaagtcaacctt- cggttgaccttgaggg ttccctaagggttggggatgacccttgggtttgtctttgggtgttaccttgagtgtctctctgtgtccctatct- gttacagtctcctaaagtatcct cctaaagtcacctcctaacgtccatcctaaagccaacacctaaagcctacacctaaagacccatcaagtcaacg- cctatcttaaagtttaa acataaagaccagacctaaagaccagacctaaagacactacataaagaccagacctaaagacgccttgttgtta- gccataaagtgataa cctttaatcattgtctttattaatacaactcactataaggagagacaacttaaagagacttaaaagattaattt- aaaatttatcaaaaagagtatt gacttaaagtctaacctataggatacttacagccatcgagagggacacggcgaatagccatcccaatcgacacc- ggggtcaaccggat aagtagacagcctgataagtcgcacgacagaaagaaattgaccgcgctaaggcccgtaaagaacgtcacgaggg- gcgcttagaggc acgcagattcaaacgtcgcaaccgcaaggcacgtaaagcacacaaagctaagcgcgaaagaatgcttgctgcgt- ggcgatgggctga acgtcaagaacggcgtaaccatgaggtagctgtagatgtactaggaagaaccaataacgctatgctctgggtca- acatgttctctgggga ctttaaggcgcttgaggaacgaatcgcgctgcactggcgtaatgctgaccggatggctatcgctaatggtctta- cgctcaacattgataag caacttgacgcaatgttaatgggctgatagtcttatcttacaggtcatctgcgggtggcctgaataggtacgat- ttactaactggaagaggc actaaatgaacacgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacact- ctggctgaccattacggt gagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagat- gtttgagcgtcaactta aagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgc- atcaacgactggtttga ggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtag- cgtacatcaccat taagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcggg- ccattgaggacgagg ctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgc- gtagggcacgtctac aagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttc- gtggcataaggaagac tctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaa- tgctggcgtagtaggtc aagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggc- atctctccgatgttcc aaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcct- ctggcgctggtgcgtact cacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgca- aaacaccgcatggaa aatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctg- cgattgagcgtgaa gaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgc- tgtgtaccgcaagg acaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataag- gccatctggttcccttac aacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggact- gcttacgctggcgaaa ggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggt- tccgttccctgagcg catcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtggg- ctgagcaagattctcc gttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttc- cgctggcgtttgacgggtc ttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttccta- gtgaaaccgttcaggac atctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagt- agttaccgtgaccgat gagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacgg- tgttactcgcagtgtg actaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagatac- cattcagccagctattg attccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatct- gtgagcgtgacggtggt agctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaaga- ctggagagattcttcg caagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcaga- cgcgcttgaacctgatg ttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacagga- gtctggtatcgctccta actttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaa- tcttttgcactgattca cgactccttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacat- atgagtcttgtgatgta ctggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggc- taaaggtaacttgaacc tccgtgacatcttagagtcggacttcgcgttcgcgtaacgccaaatcaatacgactcactatagagggacaaac- tcaaggtcattcgcaa gagtggcctttatgattgaccttcttccggttaatacgactcactataggagaaccttaaggtttaactttaag- acccttaagtgttaattagag atttaaattaaagaattactaagagaggactttaagtatgcgtaacttcgaaaagatgaccaaacgttctaacc- gtaatgctcgtgacttcga ggcaaccaaaggtcgcaagttgaataagactaagcgtgaccgctctcacaagcgtagctgggagggtcagtaag- atgggacgtttatat agtggtaatctggcagcattcaaggcagcaacaaacaagctgttccagttagacttagcggtcatttatgatga- ctggtatgatgcctatac aagaaaagattgcatacggttacgtattgaggacaggagtggaaacctgattgatactagcaccttctaccacc- acgacgaggacgttct gttcaatatgtgtactgattggttgaaccatatgtatgaccagttgaaggactggaagtaatacgactcagtat- agggacaatgcttaaggt cgctctctaggagtggccttagtcatttaaccaataggagataaacattatgatgaacattaagactaacccgt- ttaaagccgtgtctttcgta gagtctgccattaagaaggctctggataacgctgggtatcttatcgctgaaatcaagtacgatggtgtacgcgg- gaacatctgcgtagac aatactgctaacagttactggctctctcgtgtatctaaaacgattccggcactggagcacttaaacgggtttga- tgttcgctggaagcgtcta ctgaacgatgaccgttgcttctacaaagatggctttatgcttgatggggaactcatggtcaagggcgtagactt- taacacagggtccggcc tactgcgtaccaaatggactgacacgaagaaccaagagttccatgaagagttattcgttgaaccaatccgtaag- aaagataaagttccctt taagctgcacactggacaccttcacataaaactgtacgctatcctcccgctgcacatcgtggagtctggagaag- actgtgatgtcatgacg ttgctcatgcaggaacacgttaagaacatgctgcctctgctacaggaatacttccctgaaatcgaatggcaagc- ggctgaatcttacgag gtctacgatatggtagaactacagcaactgtacgagcagaagcgagcagaaggccatgagggtctcattgtgaa- agacccgatgtgtat ctataagcgcggtaagaaatctggctggtggaaaatgaaacctgagaacgaagctgacggtatcattcagggtc- tggtatggggtacaa aaggtctggctaatgaaggtaaagtgattggttttgaggtgcttcttgagagtggtcgtttagttaacgccacg- aatatctctcgcgccttaat ggatgagttcactgagacagtaaaagaggccaccctaagtcaatggggattctttagcccatacggtattggcg- acaacgatgcttgtact attaacccttacgatggctgggcgtgtcaaattagctacatggaggaaacacctgatggctctttgcggcaccc- atcgttcgtaatgttccg tggcaccgaggacaaccctcaagagaaaatgtaatcacactggctcaccttcgggtgggcctttctgcgtttat- aaggagacactttatgtt taagaaggttggtaaattccttgcggctttggcagctatcctgacgcttgcgtatattcttgcggtataccctc- aagtagcactagtagtagtt ggcgcttgttacttagcggcagtgtgtgcttgcgtgtggagtatagttaactggtaatacgactcactaaagga- ggtacacaccatgatgta cttaatgccattactcatcgtcattgtaggatgccttgcgctccactgtagcgatgatgatatgccagatggtc- acgcttaatacgactcact aaaggagacactatatgtttcgacttcattacaacaaaagcgttaagaatttcacggttcgccgtgctgaccgt- tcaatcgtatgtgcgagc gagcgccgagctaagatacctcttattggtaacacagttcctttggcaccgagcgtccacatcattatcacccg- tggtgactttgagaaag caatagacaagaaacgtccggttcttagtgtggcagtgacccgcttcccgttcgtccgtctgttactcaaacga- atcaaggaggtgttctg atgggactgttagatggtgaagcctgggaaaaagaaaacccgccagtacaagcaactgggtgtatagcttgctt- agagaaagatgacc gttatccacacacctgtaacaaaggagctaacgatatgaccgaacgtgaacaagagatgatcattaagttgata- gacaataatgaaggtc gcccagatgatttgaatggctgcggtattctctgctccaatgtcccttgccacctctgccccgcaaataacgat- caaaagataaccttaggt gaaatccgagcgatggacccacgtaaaccacatctgaataaacctgaggtaactcctacagatgaccagccttc- cgctgagacaatcga aggtgtcactaagccttcccactacatgctgtttgacgacattgaggctatcgaagtgattgctcgttcaatga- ccgttgagcagttcaagg gatactgcttcggtaacatcttaaagtacagactacgtgctggtaagaagtcagagttagcgtacttagagaaa- gacctagcgaaagcag acttctataaagaactctttgagaaacataaggataaatgttatgcataacttcaagtcaaccccacctgccga- cagcctatctgatgacttc acatcttgctcagagtggtgccgaaagatgtgggaagagacattcgacgatgcgtacatcaagctgtatgaact- ttggaaatcgagaggt caatgactatgtcaaacgtaaatacaggttcacttagtgtggacaataagaagttttgggctaccgtagagtcc- tcggagcattccttcgag gttccaatctacgctgagaccctagacgaagctctggagttagccgaatggcaatacgttccggctggctttga- ggttactcgtgtgcgtc cttgtgtagcaccgaagtaatacgactcactattagggaagactccctctgagaaaccaaacgaaacctaaagg- agattaacattatggct aagaagattttcacctctgcgctgggtaccgctgaaccttacgcttacatcgccaagccggactacggcaacga- agagcgtggctttgg gaaccctcgtggtgtctataaagttgacctgactattcccaacaaagacccgcgctgccagcgtatggtcgatg- aaatcgtgaagtgtca cgaagaggcttatgctgctgccgttgaggaatacgaagctaatccacctgctgtagctcgtggtaagaaaccgc- tgaaaccgtatgagg gtgacatgccgttcttcgataacggtgacggtacgactacctttaagttcaaatgctacgcgtctttccaagac- aagaagaccaaagagac

caagcacatcaatctggttgtggttgactcaaaaggtaagaagatggaagacgttccgattatcggtggtggct- ctaagctgaaagttaaa tattctctggttccatacaagtggaacactgctgtaggtgcgagcgttaagctgcaactggaatccgtgatgct- ggtcgaactggctacctt tggtggcggtgaagacgattgggctgacgaagttgaagagaacggctatgttgcctctggttctgccaaagcga- gcaaaccacgcgac gaagaaagctgggacgaagacgacgaagagtccgaggaagcagacgaagacggagacttctaagtggaactgcg- ggagaaaatcc ttgagcgaatcaaggtgacttcctctgggtgttgggagtggcagggcgctacgaacaataaagggtacgggcag- gtgtggtgcagcaa taccggaaaggttgtctactgtcatcgcgtaatgtctaatgctccgaaaggttctaccgtcctgcactcctgtg- ataatccattatgttgtaac cctgaacacctatccataggaactccaaaagagaactccactgacatggtaaataagggtcgctcacacaaggg- gtataaactttcagac gaagacgtaatggcaatcatggagtccagcgagtccaatgtatccttagctcgcacctatggtgtctcccaaca- gactatttgtgatatacg caaagggaggcgacatggcaggttacggcgctaaaggaatccgaaaggttggagcgtttcgctctggcctagag- gacaaggtttcaaa gcagttggaatcaaaaggtattaaattcgagtatgaagagtggaaagtgccttatgtaattccggcgagcaatc- acacttacactccagac ttcttacttccaaacggtatattcgttgagacaaagggtctgtgggaaagcgatgatagaaagaagcacttatt- aattagggagcagcacc ccgagctagacatccgtattgtcttctcaagctcacgtactaagttatacaaaggttctccaacgtcttatgga- gagttctgcgaaaagcatg gtattaagttcgctgataaactgatacctgctgagtggataaaggaacccaagaaggaggtcccctttgataga- ttaaaaaggaaaggag gaaagaaataatggctcgtgtacagtttaaacaacgtgaatctactgacgcaatctttgttcactgctcggcta- ccaagccaagtcagaatg ttggtgtccgtgagattcgccagtggcacaaagagcagggttggctcgatgtgggataccactttatcatcaag- cgagacggtactgtgg aggcaggacgagatgagatggctgtaggctctcacgctaagggttacaaccacaactctatcggcgtctgcctt- gttggtggtatcgacg ataaaggtaagttcgacgctaactttacgccagcccaaatgcaatcccttcgctcactgcttgtcacactgctg- gctaagtacgaaggcgc tggtcttcgcgcccatcatgaggtggcgccgaaggcttgcccttcgttcgaccttaagcgttggtgggagaaga- acgaactggtcacttc tgaccgtggataatgatctattggaagtcgttgcgtggatttatagaactaggagggaattgcatggacaattc- gcacgattccgatagtgt atttctttaccacattccttgtgacaactgtgggagtagtgatgggaactcgctgttctctgacggacacacgt- tctgctacgtatgcgagaa gtggactgctggtaatgaagacactaaagagagggcttcaaaacggaaaccctcaggaggtaaaccaatgactt- acaacgtgtggaac ttcggggaatccaatggacgctactccgcgttaactgcgagaggaatctccaaggaaacctgtcagaaggctgg- ctactggattgccaa agtagacggtgtgatgtaccaagtggctgactatcgggaccagaacggcaacattgtgagtcagaaggttcgag- ataaagataagaact ttaagaccactggtagtcacaagagtgacgctctgttcgggaagcacttgtggaatggtggtaagaagattgtc- gttacagaaggtgaaa tcgacatgcttaccgtgatggaacttcaagactgtaagtatcctgtagtgtcgttgggtcacggtgcctctgcc- gctaagaagacatgcgct gccaactacgaatactttgaccagttcgaacagattatcttaatgttcgatatggacgaagcagggcgcaaagc- agtcgaagaggctgca caggttctacctgctggtaaggtacgagtggcagttcttccgtgtaaggatgcaaacgagtgtcacctaaatgg- tcacgaccgtgaaatc atggagcaagtgtggaatgctggtccttggattcctgatggtgtggtatcggctctttcgttacgtgaacgaat- ccgtgagcacctatcgtc cgaggaatcagtaggtttacttttcagtggctgcactggtatcaacgataagaccttaggtgcccgtggtggtg- aagtcattatggtcacttc cggttccggtatgggtaagtcaacgttcgtccgtcaacaagctctacaatggggcacagcgatgggcaagaagg- taggcttagcgatg cttgaggagtccgttgaggagaccgctgaggaccttataggtctacacaaccgtgtccgactgagacaatccga- ctcactaaagagaga gattattgagaacggtaagttcgaccaatggttcgatgaactgttcggcaacgatacgttccatctatatgact- cattcgccgaggctgaga cggatagactgctcgctaagctggcctacatgcgctcaggcttgggctgtgacgtaatcattctagaccacatc- tcaatcgtcgtatccgct tctggtgaatccgatgagcgtaagatgattgacaacctgatgaccaagctcaaagggttcgctaagtcaactgg- ggtggtgctggtcgta atttgtcaccttaagaacccagacaaaggtaaagcacatgaggaaggtcgccccgtttctattactgacctacg- tggttctggcgcactac gccaactatctgatactattattgcccttgagcgtaatcagcaaggcgatatgcctaaccttgtcctcgttcgt- attctcaagtgccgctttact ggtgatactggtatcgctggctacatggaatacaacaaggaaaccggatggcttgaaccatcaagttactcagg- ggaagaagagtcaca ctcagagtcaacagactggtccaacgacactgacttctgacaggattcttgacagttgtttcatatgaagagat- tgttaagtcacgataatca ataggagaaatcaatatgatcgtttctgacatcgaagctaacgccctcttagagagcgtcactaagttccactg- cggggttatctacgacta ctccaccgctgagtacgtaagctaccgtccgagtgacttcggtgcgtatctggatgcgctggaagccgaggttg- cacgaggcggtcttat tgtgttccacaacggtcacaagtatgacgttcctgcattgaccaaactggcaaagttgcaattgaaccgagagt- tccaccttcctcgtgag aactgtattgacacccttgtgttgtcacgtttgattcattccaacctcaaggacaccgatatgggtcttctgcg- ttccggcaagttgcccgga aaacgctttgggtctcacgctttggaggcgtggggttatcgcttaggcgagatgaagggtgaatacaaagacga- ctttaagcgtatgcttg aagagcagggtgaagaatacgttgacggaatggagtggtggaacttcaacgaagagatgatggactataacgtt- caggacgttgtggta actaaagctctccttgagaagctactctctgacaaacattacttccctcctgagattgactttacggacgtagg- atacactacgttctggtca gaatcccttgaggccgttgacattgaacatcgtgctgcatggctgctcgctaaacaagagcgcaacgggttccc- gtttgacacaaaagca atcgaagagttgtacgtagagttagctgctcgccgctctgagttgctccgtaaattgaccgaaacgttcggctc- gtggtatcagcctaaag gtggcactgagatgttctgccatccgcgaacaggtaagccactacctaaataccctcgcattaagacacctaaa- gttggtggtatctttaa gaagcctaagaacaaggcacagcgagaaggccgtgagccttgcgaacttgatacccgcgagtacgttgctggtg- ctccttacacccca gttgaacatgttgtgtttaacccttcgtctcgtgaccacattcagaagaaactccaagaggctgggtgggtccc- gaccaagtacaccgata agggtgctcctgtggtggacgatgaggtactcgaaggagtacgtgtagatgaccctgagaagcaagccgctatc- gacctcattaaaga gtacttgatgattcagaagcgaatcggacagtctgctgagggagacaaagcatggcttcgttatgttgctgagg- atggtaagattcatggt tctgttaaccctaatggagcagttacgggtcgtgcgacccatgcgttcccaaaccttgcgcaaattccgggtgt- acgttctccttatggaga gcagtgtcgcgctgcttttggcgctgagcaccatttggatgggataactggtaagccttgggttcaggctggca- tcgacgcatccggtctt gagctacgctgcttggctcacttcatggctcgctttgataacggcgagtacgctcacgagattcttaacggcga- catccacactaagaacc agatagctgctgaactacctacccgagataacgctaagacgttcatctatgggttcctctatggtgctggtgat- gagaagattggacagatt gttggtgctggtaaagagcgcggtaaggaactcaagaagaaattccttgagaacacccccgcgattgcagcact- ccgcgagtctatcca acagacacttgtcgagtcctctcaatgggtagctggtgagcaacaagtcaagtggaaacgccgctggattaaag- gtctggatggtcgta aggtacacgttcgtagtcctcacgctgccttgaataccctactgcaatctgctggtgctctcatctgcaaactg- tggattatcaagaccgaa gagatgctcgtagagaaaggcttgaagcatggctgggatggggactttgcgtacatggcatgggtacatgatga- aatccaagtaggctg ccgtaccgaagagattgctcaggtggtcattgagaccgcacaagaagcgatgcgctgggttggagaccactgga- acttccggtgtcttc tggataccgaaggtaagatgggtcctaattgggcgatttgccactgatacaggaggctactcatgaacgaaaga- cacttaacaggtgctg cttctgaaatgctagtagcctacaaatttaccaaagctgggtacactgtctattaccctatgctgactcagagt- aaagaggacttggttgtat gtaaggatggtaaatttagtaaggttcaggttaaaacagccacaacggttcaaaccaacacaggagatgccaag- caggttaggctaggt ggatgcggtaggtccgaatataaggatggagactttgacattcttgcggttgtggttgacgaagatgtgcttat- tttcacatgggacgaagt aaaaggtaagacatccatgtgtgtcggcaagagaaacaaaggcataaaactataggagaaattattatggctat- gacaaagaaatttaaa gtgtccttcgacgttaccgcaaagatgtcgtctgacgttcaggcaatcttagagaaagatatgctgcatctatg- taagcaggtcggctcag gtgcgattgtccccaatggtaaacagaaggaaatgattgtccagttcctgacacacggtatggaaggattgatg- acattcgtagtacgtac atcatttcgtgaggccattaaggacatgcacgaagagtatgcagataaggactctttcaaacaatctcctgcaa- cagtacgggaggtgttc tgatgtctgactacctgaaagtgctgcaagcaatcaaaagttgccctaagactttccagtccaactatgtacgg- aacaatgcgagcctcgt agcggaggccgcttcccgtggtcacatctcgtgcctgactactagtggacgtaacggtggcgcttgggaaatca- ctgcttccggtactcg ctttctgaaacgaatgggaggatgtgtctaatgtctcgtgaccttgtgactattccacgcgatgtgtggaacga- tatacagggctacatcga ctctctggaacgtgagaacgatagccttaagaatcaactaatggaagctgacgaatacgtagcggaactagagg- agaaacttaatggca cttcttgaccttaaacaattctatgagttacgtgaaggctgcgacgacaagggtatccttgtgatggacggcga- ctggctggtcttccaagc tatgagtgctgctgagtttgatgcctcttgggaggaagagatttggcaccgatgctgtgaccacgctaaggccc- gtcagattcttgaggat tccattaagtcctacgagacccgtaagaaggcttgggcaggtgctccaattgtccttgcgttcaccgatagtgt- taactggcgtaaagaac tggttgacccgaactataaggctaaccgtaaggccgtgaagaaacctgtagggtactttgagttccttgatgct- ctctttgagcgcgaaga gttctattgcatccgtgagcctatgcttgagggtgatgacgttatgggagttattgcttccaatccgtctgcct- tcggtgctcgtaaggctgta atcatctcttgcgataaggactttaagaccatccctaactgtgacttcctgtggtgtaccactggtaacatcct- gactcagaccgaagagtc cgctgactggtggcacctcttccagaccatcaagggtgacatcactgatggttactcagggattgctggatggg- gtgataccgccgagg acttcttgaataacccgttcataaccgagcctaaaacgtctgtgcttaagtccggtaagaacaaaggccaagag- gttactaaatgggttaa acgcgaccctgagcctcatgagacgctttgggactgcattaagtccattggcgcgaaggctggtatgaccgaag- aggatattatcaagc agggccaaatggctcgaatcctacggttcaacgagtacaactttattgacaaggagatttacctgtggagaccg- tagcgtatattggtctg ggtctttgtgttctcggagtgtgcctcatttcgtggggcctttgggacttagccagaataatcaagtcgttaca- cgacactaagtgataaact

caaggtccctaaattaatacgactcactatagggagataggggcctttacgattattactttaagatttaactc- taagaggaatctttattatgtt aacacctattaaccaattacttaagaaccctaacgatattccagatgtacctcgtgcaaccgctgagtatctac- aggttcgattcaactatgc gtacctcgaagcgtctggtcatataggacttatgcgtgctaatggttgtagtgaggcccacatcttgggtttca- ttcagggcctacagtatgc ctctaacgtcattgacgagattgagttacgcaaggaacaactaagagatgatggggaggattgacactatgtgt- ttctcaccgaaaattaa aactccgaagatggataccaatcagattcgagccgttgagccagcgcctctgacccaagaagtgtcaagcgtgg- agttcggtgggtctt ctgatgagacggataccgagggcaccgaagtgtctggacgcaaaggcctcaaggtcgaacgtgatgattccgta- gcgaagtctaaag ccagcggcaatggctccgctcgtatgaaatcttccatccgtaagtccgcatttggaggtaagaagtgatgtctg- agttcacatgtgtggag gctaagagtcgcttccgtgcaatccggtggactgtggaacaccttgggttgcctaaaggattcgaaggacactt- tgtgggctacagcctct acgtagacgaagtgatggacatgtctggttgccgtgaagagtacattctggactctaccggaaaacatgtagcg- tacttcgcgtggtgcg taagctgtgacattcaccacaaaggagacattctggatgtaacgtccgttgtcattaatcctgaggcagactct- aagggcttacagcgattc ctagcgaaacgctttaagtaccttgcggaactccacgattgcgattgggtgtctcgttgtaagcatgaaggcga- gacaatgcgtgtatactt taaggaggtataagttatgggtaagaaagttaagaaggccgtgaagaaagtcaccaagtccgttaagaaagtcg- ttaaggaaggggctc gtccggttaaacaggttgctggcggtctagctggtctggctggtggtactggtgaagcacagatggtggaagta- ccacaagctgccgca cagattgttgacgtacctgagaaagaggtttccactgaggacgaagcacagacagaaagcggacgcaagaaagc- tcgtgctggcggt aagaaatccttgagtgtagcccgtagctccggtggcggtatcaacatttaatcaggaggttatcgtggaagact- gcattgaatggaccgg aggtgtcaactctaagggttatggtcgtaagtgggttaatggtaaacttgtgactccacataggcacatctatg- aggagacatatggtcca gttccaacaggaattgtggtgatgcatatctgcgataaccctaggtgctataacataaagcaccttacgcttgg- aactccaaaggataattc cgaggacatggttaccaaaggtagacaggctaaaggagaggaactaagcaagaaacttacagagtcagacgttc- tcgctatacgctctt caaccttaagccaccgctccttaggagaactgtatggagtcagtcaatcaaccataacgcgaatactacagcgt- aagacatggagacac atttaatggctgagaaacgaacaggacttgcggaggatggcgcaaagtctgtctatgagcgtttaaagaacgac- cgtgctccctatgaga cacgcgctcagaattgcgctcaatataccatcccatcattgttccctaaggactccgataacgcctctacagat- tatcaaactccgtggcaa gccgtgggcgctcgtggtctgaacaatctagcctctaagctcatgctggctctattccctatgcagacttggat- gcgacttactatatctgaa tatgaagcaaagcagttactgagcgaccccgatggactcgctaaggtcgatgagggcctctcgatggtagagcg- tatcatcatgaacta cattgagtctaacagttaccgcgtgactctctttgaggctctcaaacagttagtcgtagctggtaacgtcctgc- tgtacctaccggaaccgg aagggtcaaactataatcccatgaagctgtaccgattgtcttcttatgtggtccaacgagacgcattcggcaac- gttctgcaaatggtgact cgtgaccagatagcttttggtgctctccctgaggacatccgtaaggctgtagaaggtcaaggtggtgagaagaa- agctgatgagacaat cgacgtgtacactcacatctatctggatgaggactcaggtgaatacctccgatacgaagaggtcgagggtatgg- aagtccaaggctccg atgggacttatcctaaagaggcttgcccatacatcccgattcggatggtcagactagatggtgaatcctacggt- cgttcgtacattgagga atacttaggtgacttacggtcccttgaaaatctccaagaggctatcgtcaagatgtccatgattagctctaagg- ttatcggcttagtgaatcct gctggtatcacccagccacgccgactgaccaaagctcagactggtgacttcgttactggtcgtccagaagacat- ctcgttcctccaactg gagaagcaagcagactttactgtagctaaagccgtaagtgacgctatcgaggctcgcctttcgtttgcctttat- gttgaactctgcggttca gcgtacaggtgaacgtgtgaccgccgaagagattcggtatgtagcttctgaacttgaagatactttaggtggtg- tctactctatcctttctca agaattacaattgcctctggtacgagtgctcttgaagcaactacaagccacgcaacagattcctgagttaccta- aggaagccgtagagcc aaccattagtacaggtctggaagcaattggtcgaggacaagaccttgataagctggagcggtgtgtcactgcgt- gggctgcactggcac ctatgcgggacgaccctgatattaaccttgcgatgattaagttacgtattgccaacgctatcggtattgacact- tctggtattctactcaccga agaacagaagcaacagaagatggcccaacagtctatgcaaatgggtatggataatggtgctgctgcgctggctc- aaggtatggctgca caagctacagcttcacctgaggctatggctgctgccgctgattccgtaggtttacagccgggaatttaatacga- ctcactatagggagacc tcatctttgaaatgagcgatgacaagaggttggagtcctcggtcttcctgtagttcaactttaaggagacaata- ataatggctgaatctaatg cagacgtatatgcatcttttggcgtgaactccgctgtgatgtctggtggttccgttgaggaacatgagcagaac- atgctggctcttgatgttg ctgcccgtgatggcgatgatgcaatcgagttagcgtcagacgaagtggaaacagaacgtgacctgtatgacaac- tctgacccgttcggt caagaggatgacgaaggccgcattcaggttcgtatcggtgatggctctgagccgaccgatgtggacactggaga- agaaggcgttgag ggcaccgaaggttccgaagagtttaccccactgggcgagactccagaagaactggtagctgcctctgagcaact- tggtgagcacgaag agggcttccaagagatgattaacattgctgctgagcgtggcatgagtgtcgagaccattgaggctatccagcgt- gagtacgaggagaac gaagagttgtccgccgagtcctacgctaagctggctgaaattggctacacgaaggctttcattgactcgtatat- ccgtggtcaagaagctc tggtggagcagtacgtaaacagtgtcattgagtacgctggtggtcgtgaacgttttgatgcactgtataaccac- cttgagacgcacaaccc tgaggctgcacagtcgctggataatgcgttgaccaatcgtgacttagcgaccgttaaggctatcatcaacttgg- ctggtgagtctcgcgct aaggcgttcggtcgtaagccaactcgtagtgtgactaatcgtgctattccggctaaacctcaggctaccaagcg- tgaaggctttgcggac cgtagcgagatgattaaagctatgagtgaccctcggtatcgcacagatgccaactatcgtcgtcaagtcgaaca- gaaagtaatcgattcg aacttctaactagatctcattatcatatggctagcatgactggtggacagcaaatgggtactaaccaaggtaaa- ggtgtagttgctgctgga gataaactggcgttgttcttgaaggtatttggcggtgaagtcctgactgcgttcgctcgtacctccgtgaccac- ttctcgccacatggtacgt tccatctccagcggtaaatccgctcagttccctgttctgggtcgcactcaggcagcgtatctggctccgggcga- gaacctcgacgataaa cgtaaggacatcaaacacaccgagaaggtaatcaccattgacggtctcctgacggctgacgttctgatttatga- tattgaggacgcgatg aaccactacgacgttcgctctgagtatacctctcagttgggtgaatctctggcgatggctgcggatggtgcggt- tctggctgagattgccg gtctgtgtaacgtggaaagcaaatataatgagaacatcgagggcttaggtactgctaccgtaattgagaccact- cagaacaaggccgca cttaccgaccaagttgcgctgggtaaggagattattgcggctctgactaaggctcgtgcggctctgaccaagaa- ctatgttccggctgctg accgtgtgttctactgtgacccagatagctactctgcgattctggcagcactgatgccgaacgcagcaaactac- gctgctctgattgaccc tgagaagggttctatccgcaacgttatgggctttgaggttgtagaagttccgcacctcaccgctggtggtgctg- gtaccgctcgtgaggg cactactggtcagaagcacgtcttccctgccaataaaggtgagggtaatgtcaaggttgctaaggacaacgtta- tcggcctgttcatgcac cgctctgcggtaggtactgttaagctgcgtgacttggctctggagcgcgctcgccgtgctaacttccaagcgga- ccagattatcgctaagt acgcaatgggccacggtggtcttcgcccagaagctgcaggagctgtcgtattccagtcaggtgtgatgctcggg- gatctcgagggggc ccaggcggccgcactcgactcggtaccccgatggataatgatcatctggatattgatgttgatattactgaacc- gtcatttgaacatttaact attgcgacagtcaatgaacgccgaatgagaattgagattgaaaatcaagcaatttctctgtcttaaaatctatt- gagatattctatcactcaaa tagcaatataaggactctctatgaaatttggaaactttttgcttacataccaacctccccaattttctcaaaca- gaggtaatgaaacgtttggtt aaattaggtcgcatctctgaggagtgtggttttgataccgtatggttactggagcatcatttcacggagtttgg- tttgcttggtaacccttatgt cgctgctgcatatttacttggcgcgactaaaaaattgaatgtaggaactgccgctattgttcttcccacagccc- atccagtacgccaacttg aagatgtgaatttattggatcaaatgtcaaaaggacgatttcggtttggtatttgccgagggctttacaacaag- gactttcgcgtattcggca cagatatgaataacagtcgcgccttagcggaatgctggtacgggctgataaagaatggcatgacagagggatat- atggaagctgataat gaacatatcaagttccataaggtaaaagtaaaccccgcggcgtatagcagaggtggcgcaccggtttatgtggt- ggctgaatcagcttcg acgactgagtgggctgctcaatttggcctaccgatgatattaagttggattataaatactaacgaaaagaaagc- acaacttgagctttataat gaagtggctcaagaatatgggcacgatattcataatatcgaccattgcttatcatatataacatctgtagatca- tgactcaattaaagcgaaa gagatttgccggaaatttctggggcattggtatgattcttatgtgaatgctacgactatttttgatgattcaga- ccaaacaagaggttatgattt caataaagggcagtggcgtgactttgtattaaaaggacataaagatactaatcgccgtattgattacagttacg- aaatcaatcccgtggga acgccgcaggaatgtattgacataattcaaaaagacattgatgctacaggaatatcaaatatttgttgtggatt- tgaagctaatggaacagta gacgaaattattgcttccatgaagctcttccagtctgatgtcatgccatttcttaaagaaaaacaacgttcgct- attatattagctaaggagaa agaaatgaaatttggattgttcttccttaacttcatcaattcaacaactgttcaagaacaaagtatagttcgca- tgcaggaaataacggagtat gttgataagttgaattttgaacagattttagtgtatgaaaatcatttttcagataatggtgttgtcggcgctcc- tctgactgtttctggttttctgct cggtttaacagagaaaattaaaattggttcattaaatcacatcattacaactcatcatcctgtcgccatagcgg- aggaagcttgcttattggat cagttaagtgaagggagatttattttagggtttagtgattgcgaaaaaaaagatgaaatgcatttttttaatcg- cccggttgaatatcaacagc aactatttgaagagtgttatgaaatcattaacgatgctttaacaacaggctattgtaatccagataacgatttt- tatagcttccctaaaatatctg taaatccccatgcttatacgccaggcggacctcggaaatatgtaacagcaaccagtcatcatattgttgagtgg- gcggccaaaaaaggta ttcctctcatctttaagtgggatgattctaatgatgttagatatgaatatgctgaaagatataaagccgttgcg- gataaatatgacgttgaccta tcagagatagaccatcagttaatgatattagttaactataacgaagatagtaataaagctaaacaagagacgcg- tgcatttattagtgattat gttcttgaaatgcaccctaatgaaaatttcgaaaataaacttgaagaaataattgcagaaaacgctgtcggaaa- ttatacggagtgtataact gcggctaagttggcaattgaaaagtgtggtgcgaaaagtgtattgctgtcctttgaaccaatgaatgatttgat-

gagccaaaaaaatgtaat caatattgttgatgataatattaagaagtaccacatggaatatacctaatagatttcgagttgcagcgaggcgg- caagtgaacgaatcccc aggagcatagataactatgtgactggggtgagtgaaagcagccaacaaagcagcagcttgaaagatgaagggta- taaaagagtatgac agcagtgctgccatactttctaatattatcttgaggagtaaaacaggtatgacttcatatgttgataaacaaga- aattacagcaagctcagaa attgatgatttgattttttcgagcgatccatctcgaattcgagctccgtcgacaagcttgcggccgcactcgag- taactagttaaccccttgg ggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatgcgctcatacgatatgaacgttga- gactgccgctgagttatc agctgtgaacgacattctggcgtctatcggtgaacctccggtatcaacgctggaaggtgacgctaacgcagatg- cagcgaacgctcgg cgtattctcaacaagattaaccgacagattcaatctcgtggatggacgttcaacattgaggaaggcataacgct- actacctgatgtttactc caacctgattgtatacagtgacgactatttatccctaatgtctacttccggtcaatccatctacgttaaccgag- gtggctatgtgtatgaccga acgagtcaatcagaccgctttgactctggtattactgtgaacattattcgtctccgcgactacgatgagatgcc- tgagtgcttccgttactgg attgtcaccaaggcttcccgtcagttcaacaaccgattctttggggcaccggaagtagagggtgtactccaaga- agaggaagatgaggc tagacgtctctgcatggagtatgagatggactacggtgggtacaatatgctggatggagatgcgttcacttctg- gtctactgactcgctaac attaataaataaggaggctctaatggcactcattagccaatcaatcaagaacttgaagggtggtatcagccaac- agcctgacatccttcgtt atccagaccaagggtcacgccaagttaacggttggtcttcggagaccgagggcctccaaaagcgtccacctctt- gttttcttaaatacact tggagacaacggtgcgttaggtcaagctccgtacatccacctgattaaccgagatgagcacgaacagtattacg- ctgtgttcactggtag cggaatccgagtgttcgacctttctggtaacgagaagcaagttaggtatcctaacggttccaactacatcaaga- ccgctaatccacgtaac gacctgcgaatggttactgtagcagactatacgttcatcgttaaccgtaacgttgttgcacagaagaacacaaa- gtctgtcaacttaccgaa ttacaaccctaatcaagacggattgattaacgttcgtggtggtcagtatggtagggaactaattgtacacatta- acggtaaagacgttgcga agtataagataccagatggtagtcaacctgaacacgtaaacaatacggatgcccaatggttagctgaagagtta- gccaagcagatgcgc actaacttgtctgattggactgtaaatgtagggcaagggttcatccatgtgaccgcacctagtggtcaacagat- tgactccttcacgactaa agatggctacgcagaccagttgattaaccctgtgacccactacgctcagtcgttctctaagctgccacctaatg- ctcctaacggctacatg gtgaaaatcgtaggggacgcctctaagtctgccgaccagtattacgttcggtatgacgctgagcggaaagtttg- gactgagactttaggtt ggaacactgaggaccaagttctatgggaaaccatgccacacgctcttgtgcgagccgctgacggtaatttcgac- ttcaagtggcttgagt ggtctcctaagtcttgtggtgacgttgacaccaacccttggccttcttttgttggttcaagtattaacgatgtg- ttcttcttccgtaaccgcttag gattccttagtggggagaacatcatattgagtcgtacagccaaatacttcaacttctaccctgcgtccattgcg- aaccttagtgatgacgac cctatagacgtagctgtgagtaccaaccgaatagcaatccttaagtacgccgttccgttctcagaagagttact- catctggtccgatgaag cacaattcgtcctgactgcctcgggtactctcacatctaagtcggttgagttgaacctaacgacccagtttgac- gtacaggaccgagcga gaccttttgggattgggcgtaatgtctactttgctagtccgaggtccagcttcacgtccatccacaggtactac- gctgtgcaggatgtcagtt ccgttaagaatgctgaggacattacatcacacgttcctaactacatccctaatggtgtgttcagtatttgcgga- agtggtacggaaaacttct gttcggtactatctcacggggaccctagtaaaatcttcatgtacaaattcctgtacctgaacgaagagttaagg- caacagtcgtggtctcatt gggactttggggaaaacgtacaggttctagcttgtcagagtatcagctcagatatgtatgtgattcttcgcaat- gagttcaatacgttcctag ctagaatctctttcactaagaacgccattgacttacagggagaaccctatcgtgcctttatggacatgaagatt- cgatacacgattcctagtg gaacatacaacgatgacacattcactacctctattcatattccaacaatttatggtgcaaacttcgggaggggc- aaaatcactgtattggag cctgatggtaagataaccgtgtttgagcaacctacggctgggtggaatagcgacccttggctgagactcagcgg- taacttggagggacg catggtgtacattgggttcaacattaacttcgtatatgagttctctaagttcctcatcaagcagactgccgacg- acgggtctacctccacgga agacattgggcgcttacagttacgccgagcgtgggttaactacgagaactctggtacgtttgacatttatgttg- agaaccaatcgtctaact ggaagtacacaatggctggtgcccgattaggctctaacactctgagggctgggagactgaacttagggaccgga- caatatcgattccct gtggttggtaacgccaagttcaacactgtatacatcttgtcagatgagactacccctctgaacatcattgggtg- tggctgggaaggtaacta cttacggagaagttccggtatttaattaaatattctccctgtggtggctcgaaattaatacgactcactatagg- gagaacaatacgactacgg gagggttttcttatgatgactataagacctactaaaagtacagactttgaggtattcactccggctcaccatga- cattcttgaagctaaggct gctggtattgagccgagtttccctgatgcttccgagtgtgtcacgttgagcctctatgggttccctctagctat- cggtggtaactgcgggga ccagtgctggttcgttacgagcgaccaagtgtggcgacttagtggaaaggctaagcgaaagttccgtaagttaa- tcatggagtatcgcga taagatgcttgagaagtatgatactctttggaattacgtatgggtaggcaatacgtcccacattcgtttcctca- agactatcggtgcggtattc catgaagagtacacacgagatggtcaatttcagttatttacaatcacgaaaggaggataaccatatgtgttggg- cagccgcaatacctatc gctatatctggcgctcaggctatcagtggtcagaacgctcaggccaaaatgattgccgctcagaccgctgctgg- tcgtcgtcaagctatg gaaatcatgaggcagacgaacatccagaatgctgacctatcgttgcaagctcgaagtaaacttgaggaagcgtc- cgccgagttgacctc acagaacatgcagaaggtccaagctattgggtctatccgagcggctatcggagagagtatgcttgaaggttcct- caatggaccgcattaa gcgagtcacagaaggacagttcattcgggaagccaatatggtaactgagaactatcgccgtgactaccaagcaa- tcttcgcacagcaac ttggtggtactcaaagtgctgcaagtcagattgacgaaatctataagagcgaacagaaacagaagagtaagcta- cagatggttctggac ccactggctatcatggggtcttccgctgcgagtgcttacgcatccggtgcgttcgactctaagtccacaactaa- ggcacctattgttgccg ctaaaggaaccaagacggggaggtaatgagctatgagtaaaattgaatctgcccttcaagcggcacaaccggga- ctctctcggttacgt ggtggtgctggaggtatgggctatcgtgcagcaaccactcaggccgaacagccaaggtcaagcctattggacac- cattggtcggttcg ctaaggctggtgccgatatgtataccgctaaggaacaacgagcacgagacctagctgatgaacgctctaacgag- attatccgtaagctg acccctgagcaacgtcgagaagctctcaacaacgggacccttctgtatcaggatgacccatacgctatggaagc- actccgagtcaaga ctggtcgtaacgctgcgtatcttgtggacgatgacgttatgcagaagataaaagagggtgtcttccgtactcgc- gaagagatggaagagt atcgccatagtcgccttcaagagggcgctaaggtatacgctgagcagttcggcatcgaccctgaggacgttgat- tatcagcgtggtttca acggggacattaccgagcgtaacatctcgctgtatggtgcgcatgataacttcttgagccagcaagctcagaag- ggcgctatcatgaac agccgagtggaactcaacggtgtccttcaagaccctgatatgctgcgtcgtccagactctgctgacttctttga- gaagtatatcgacaacg gtctggttactggcgcaatcccatctgatgctcaagccacacagcttataagccaagcgttcagtgacgcttct- agccgtgctggtggtgc tgacttcctgatgcgagtcggtgacaagaaggtaacacttaacggagccactacgacttaccgagagttgattg- gtgaggaacagtgga acgctctcatggtcacagcacaacgttctcagtttgagactgacgcgaagctgaacgagcagtatcgcttgaag- attaactctgcgctga accaagaggacccaaggacagcttgggagatgcttcaaggtatcaaggctgaactagataaggtccaacctgat- gagcagatgacacc acaacgtgagtggctaatctccgcacaggaacaagttcagaatcagatgaacgcatggacgaaagctcaggcca- aggctctggacgat tccatgaagtcaatgaacaaacttgacgtaatcgacaagcaattccagaagcgaatcaacggtgagtgggtctc- aacggattttaaggat atgccagtcaacgagaacactggtgagttcaagcatagcgatatggttaactacgccaataagaagctcgctga- gattgacagtatggac attccagacggtgccaaggatgctatgaagttgaagtaccttcaagcggactctaaggacggagcattccgtac- agccatcggaaccat ggtcactgacgctggtcaagagtggtctgccgctgtgattaacggtaagttaccagaacgaaccccagctatgg- atgctctgcgcagaat ccgcaatgctgaccctcagttgattgctgcgctatacccagaccaagctgagctattcctgacgatggacatga- tggacaagcagggtat tgaccctcaggttattcttgatgccgaccgactgactgttaagcggtccaaagagcaacgctttgaggatgata- aagcattcgagtctgca ctgaatgcatctaaggctcctgagattgcccgtatgccagcgtcactgcgcgaatctgcacgtaagatttatga- ctccgttaagtatcgctc ggggaacgaaagcatggctatggagcagatgaccaagttccttaaggaatctacctacacgttcactggtgatg- atgttgacggtgatac cgttggtgtgattcctaagaatatgatgcaggttaactctgacccgaaatcatgggagcaaggtcgggatattc- tggaggaagcacgtaa gggaatcattgcgagcaacccttggataaccaataagcaactgaccatgtattctcaaggtgactccatttacc- ttatggacaccacaggt caagtcagagtccgatacgacaaagagttactctcgaaggtctggagtgagaaccagaagaaactcgaagagaa- agctcgtgagaag gctctggctgatgtgaacaagcgagcacctatagttgccgctacgaaggcccgtgaagctgctgctaaacgagt- ccgagagaaacgta aacagactcctaagttcatctacggacgtaaggagtaactaaaggctacataaggaggccctaaatggataagt- acgataagaacgtac caagtgattatgatggtctgttccaaaaggctgctgatgccaacggggtctcttatgaccttttacgtaaagtc- gcttggacagaatcacgat ttgtgcctacagcaaaatctaagactggaccattaggcatgatgcaatttaccaaggcaaccgctaaggccctc- ggtctgcgagttaccg atggtccagacgacgaccgactgaaccctgagttagctattaatgctgccgctaagcaacttgcaggtctggta- gggaagtttgatggcg atgaactcaaagctgcccttgcgtacaaccaaggcgagggacgcttgggtaatccacaacttgaggcgtactct- aagggagacttcgca tcaatctctgaggagggacgtaactacatgcgtaaccttctggatgttgctaagtcacctatggctggacagtt- ggaaacttttggtggcat aaccccaaagggtaaaggcattccggctgaggtaggattggctggaattggtcacaagcagaaagtaacacagg- aacttcctgagtcc acaagttttgacgttaagggtatcgaacaggaggctacggcgaaaccattcgccaaggacttttgggagaccca- cggagaaacacttga cgagtacaacagtcgttcaaccttcttcggattcaaaaatgctgccgaagctgaactctccaactcagtcgctg- ggatggctttccgtgctg

gtcgtctcgataatggttttgatgtgtttaaagacaccattacgccgactcgctggaactctcacatctggact- ccagaggagttagagaag attcgaacagaggttaagaaccctgcgtacatcaacgttgtaactggtggttcccctgagaacctcgatgacct- cattaaattggctaacg agaactttgagaatgactcccgcgctgccgaggctggcctaggtgccaaactgagtgctggtattattggtgct- ggtgtggacccgctta gctatgttcctatggtcggtgtcactggtaagggctttaagttaatcaataaggctcttgtagttggtgccgaa- agtgctgctctgaacgttgc atccgaaggtctccgtacctccgtagctggtggtgacgcagactatgcgggtgctgccttaggtggctttgtgt- ttggcgcaggcatgtct gcaatcagtgacgctgtagctgctggactgaaacgcagtaaaccagaagctgagttcgacaatgagttcatcgg- tcctatgatgcgattg gaagcccgtgagacagcacgaaacgccaactctgcggacctctctcggatgaacactgagaacatgaagtttga- aggtgaacataatg gtgtcccttatgaggacttaccaacagagagaggtgccgtggtgttacatgatggctccgttctaagtgcaagc- aacccaatcaacccta agactctaaaagagttctccgaggttgaccctgagaaggctgcgcgaggaatcaaactggctgggttcaccgag- attggcttgaagacc ttggggtctgacgatgctgacatccgtagagtggctatcgacctcgttcgctctcctactggtatgcagtctgg- tgcctcaggtaagttcgg tgcaacagcttctgacatccatgagagacttcatggtactgaccagcgtacttataatgacttgtacaaagcaa- tgtctgacgctatgaaag accctgagttctctactggcggcgctaagatgtcccgtgaagaaactcgatacactatctaccgtagagcggca- ctagctattgagcgtc cagaactacagaaggcactcactccgtctgagagaatcgttatggacatcattaagcgtcactttgacaccaag- cgtgaacttatggaaa acccagcaatattcggtaacacaaaggctgtgagtatcttccctgagagtcgccacaaaggtacttacgttcct- cacgtatatgaccgtcat gccaaggcgctgatgattcaacgctacggtgccgaaggtttgcaggaagggattgcccgctcatggatgaacag- ctacgtctccagac ctgaggtcaaggccagagtcgatgagatgcttaaggaattacacggggtgaaggaagtaacaccagagatggta- gagaagtacgctat ggataaggcttatggtatctcccactcagaccagttcaccaacagttccataatagaagagaacattgagggct- tagtaggtatcgagaat aactcattccttgaggcacgtaacttgtttgattcggacctatccatcactatgccagacggacagcaattctc- agtgaatgacctaaggga cttcgatatgttccgcatcatgccagcgtatgaccgccgtgtcaatggtgacatcgccatcatggggtctactg- gtaaaaccactaaggaa cttaaggatgagattttggctctcaaagcgaaagctgagggagacggtaagaagactggcgaggtacatgcttt- aatggataccgttaag attcttactggtcgtgctagacgcaatcaggacactgtgtgggaaacctcactgcgtgccatcaatgacctagg- gttcttcgctaagaacg cctacatgggtgctcagaacattacggagattgctgggatgattgtcactggtaacgttcgtgctctagggcat- ggtatcccaattctgcgt gatacactctacaagtctaaaccagtttcagctaaggaactcaaggaactccatgcgtctctgttcgggaagga- ggtggaccagttgattc ggcctaaacgtgctgacattgtgcagcgcctaagggaagcaactgataccggacctgccgtggcgaacatcgta- gggaccttgaagta ttcaacacaggaactggctgctcgctctccgtggactaagctactgaacggaaccactaactaccttctggatg- ctgcgcgtcaaggtat gcttggggatgttattagtgccaccctaacaggtaagactacccgctgggagaaagaaggcttccttcgtggtg- cctccgtaactcctga gcagatggctggcatcaagtctctcatcaaggaacatatggtacgcggtgaggacgggaagtttaccgttaagg- acaagcaagcgttct ctatggacccacgggctatggacttatggagactggctgacaaggtagctgatgaggcaatgctgcgtccacat- aaggtgtccttacag gattcccatgcgttcggagcactaggtaagatggttatgcagtttaagtctttcactatcaagtcccttaactc- taagttcctgcgaaccttcta tgatggatacaagaacaaccgagcgattgacgctgcgctgagcatcatcacctctatgggtctcgctggtggtt- tctatgctatggctgca cacgtcaaagcatacgctctgcctaaggagaaacgtaaggagtacttggagcgtgcactggacccaaccatgat- tgcccacgctgcgtt atctcgtagttctcaattgggtgctcctttggctatggttgacctagttggtggtgttttagggttcgagtcct- ccaagatggctcgctctacga ttctacctaaggacaccgtgaaggaacgtgacccaaacaaaccgtacacctctagagaggtaatgggcgctatg- ggttcaaaccttctg gaacagatgccttcggctggctttgtggctaacgtaggggctaccttaatgaatgctgctggcgtggtcaactc- acctaataaagcaacc gagcaggacttcatgactggtcttatgaactccacaaaagagttagtaccgaacgacccattgactcaacagct- tgtgttgaagatttatga ggcgaacggtgttaacttgagggagcgtaggaaataatacgactcactatagggagaggcgaaataatcttctc- cctgtagtctcttagat ttactttaaggaggtcaaatggctaacgtaattaaaaccgttttgacttaccagttagatggctccaatcgtga- ttttaatatcccgtttgagtat ctagcccgtaagttcgtagtggtaactcttattggtgtagaccgaaaggtccttacgattaatacagactatcg- ctttgctacacgtactacta tctctctgacaaaggcttggggtccagccgatggctacacgaccatcgagttacgtcgagtaacctccactacc- gaccgattggttgactt tacggatggttcaatcctccgcgcgtatgaccttaacgtcgctcagattcaaacgatgcacgtagcggaagagg- cccgtgacctcactac ggatactatcggtgtcaataacgatggtcacttggatgctcgtggtcgtcgaattgtgaacctagcgaacgccg- tggatgaccgcgatgc tgttccgtttggtcaactaaagaccatgaaccagaactcatggcaagcacgtaatgaagccttacagttccgta- atgaggctgagactttc agaaaccaagcggagggctttaagaacgagtccagtaccaacgctacgaacacaaagcagtggcgcgatgagac- caagggtttccg agacgaagccaagcggttcaagaatacggctggtcaatacgctacatctgctgggaactctgcttccgctgcgc- atcaatctgaggtaaa cgctgagaactctgccacagcatccgctaactctgctcatttggcagaacagcaagcagaccgtgcggaacgtg- aggcagacaagctg gaaaattacaatggattggctggtgcaattgataaggtagatggaaccaatgtgtactggaaaggaaatattca- cgctaacgggcgccttt acatgaccacaaacggttttgactgtggccagtatcaacagttctttggtggtgtcactaatcgttactctgtc- atggagtggggagatgag aacggatggctgatgtatgttcaacgtagagagtggacaacagcgataggcggtaacatccagttagtagtaaa- cggacagatcatcac ccaaggtggagccatgaccggtcagctaaaattgcagaatgggcatgttcttcaattagagtccgcatccgaca- aggcgcactatattct atctaaagatggtaacaggaataactggtacattggtagagggtcagataacaacaatgactgtaccttccact- cctatgtacatggtacg accttaacactcaagcaggactatgcagtagttaacaaacacttccacgtaggtcaggccgttgtggccactga- tggtaatattcaaggta ctaagtggggaggtaaatggctggatgcttacctacgtgacagcttcgttgcgaagtccaaggcgtggactcag- gtgtggtctggtagtg ctggcggtggggtaagtgtgactgtttcacaggatctccgcttccgcaatatctggattaagtgtgccaacaac- tcttggaacttcttccgta ctggccccgatggaatctacttcatagcctctgatggtggatggttacgattccaaatacactccaacggtctc- ggattcaagaatattgca gacagtcgttcagtacctaatgcaatcatggtggagaacgagtaattggtaaatcacaaggaaagacgtgtagt- ccacggatggactctc aaggaggtacaaggtgctatcattagactttaacaacgaattgattaaggctgctccaattgttgggacgggtg- tagcagatgttagtgctc gactgttctttgggttaagccttaacgaatggttctacgttgctgctatcgcctacacagtggttcagattggt- gccaaggtagtcgataaga tgattgactggaagaaagccaataaggagtgatatgtatggaaaaggataagagccttattacattcttagaga- tgttggacactgcgatg gctcagcgtatgcttgcggacctttcggaccatgagcgtcgctctccgcaactctataatgctattaacaaact- gttagaccgccacaagtt ccagattggtaagttgcagccggatgttcacatcttaggtggccttgctggtgctcttgaagagtacaaagaga- aagtcggtgataacggt cttacggatgatgatatttacacattacagtgatatactcaaggccactacagatagtggtctttatggatgtc- attgtctatacgagatgctcc tacgtgaaatctgaaagttaacgggaggcattatgctagaatttttacgtaagctaatcccttgggttctcgct- gggatgctattcgggttag gatggcatctagggtcagactcaatggacgctaaatggaaacaggaggtacacaatgagtacgttaagagagtt- gaggctgcgaagag cactcaaagagcaatcgatgcggtatctgctaagtatcaagaagaccttgccgcgctggaagggagcactgata- ggattatttctgatttg cgtagcgacaataagcggttgcgcgtcagagtcaaaactaccggaacctccgatggtcagtgtggattcgagcc- tgatggtcgagccg aacttgacgaccgagatgctaaacgtattctcgcagtgacccagaagggtgacgcatggattcgtgcgttacag- gatactattcgtgaact gcaacgtaagtaggaaatcaagtaaggaggcaatgtgtctactcaatccaatcgtaatgcgctcgtagtggcgc- aactgaaaggagact tcgtggcgttcctattcgtcttatggaaggcgctaaacctaccggtgcccactaagtgtcagattgacatggct- aaggtgctggcgaatgg agacaacaagaagttcatcttacaggctttccgtggtatcggtaagtcgttcatcacatgtgcgttcgttgtgt- ggtccttatggagagaccc tcagttgaagatacttatcgtatcagcctctaaggagcgtgcagacgctaactccatctttattaagaacatca- ttgacctgctgccattccta tctgagttaaagccaagacccggacagcgtgactcggtaatcagctttgatgtaggcccagccaatcctgacca- ctctcctagtgtgaaat cagtaggtatcactggtcagttaactggtagccgtgctgacattatcattgcggatgacgttgagattccgtct- aacagcgcaactatgggt gcccgtgagaagctatggactctggttcaggagttcgctgcgttacttaaaccgctgccttcctctcgcgttat- ctaccttggtacacctcag acagagatgactctctataaggaacttgaggataaccgtgggtacacaaccattatctggcctgctctgtaccc- aaggacacgtgaagag aacctctattactcacagcgtcttgctcctatgttacgcgctgagtacgatgagaaccctgaggcacttgctgg- gactccaacagacccag tgcgctttgaccgtgatgacctgcgcgagcgtgagttggaatacggtaaggctggctttacgctacagttcatg- cttaaccctaaccttagt gatgccgagaagtacccgctgaggcttcgtgacgctatcgtagcggccttagacttagagaaggccccaatgca- ttaccagtggcttcc gaaccgtcagaacatcattgaggaccttcctaacgttggccttaagggtgatgacctgcatacgtaccacgatt- gttccaacaactcaggt cagtaccaacagaagattctggtcattgaccctagtggtcgcggtaaggacgaaacaggttacgctgtgctgta- cacactgaacggttac atctaccttatggaagctggaggtttccgtgatggctactccgataagacccttgagttactcgctaagaaggc- aaagcaatggggagtc cagacggttgtctacgagagtaacttcggtgacggtatgttcggtaaggtattcagtcctatccttcttaaaca- ccacaactgtgcgatgga agagattcgtgcccgtggtatgaaagagatgcgtatttgcgatacccttgagccagtcatgcagactcaccgcc- ttgtaattcgtgatgag gtcattagggccgactaccagtccgctcgtgacgtagacggtaagcatgacgttaagtactcgttgttctacca- gatgacccgtatcactc gtgagaaaggcgctctggctcatgatgaccgattggatgcccttgcgttaggcattgagtatctccgtgagtcc- atgcagttggattccgtt

aaggtcgagggtgaagtacttgctgacttccttgaggaacacatgatgcgtcctacggttgctgctacgcatat- cattgagatgtctgtggg aggagttgatgtgtactctgaggacgatgagggttacggtacgtctttcattgagtggtgatttatgcattagg- actgcatagggatgcact atagaccacggatggtcagttctttaagttactgaaaagacacgataaattaatacgactcactatagggagag- gagggacgaaaggtta ctatatagatactgaatgaatacttatagagtgcataaagtatgcataatggtgtacctagagtgacctctaag- aatggtgattatattgtatta gtatcaccttaacttaaggaccaacataaagggaggagactcatgttccgcttattgttgaacctactgcggca- tagagtcacctaccgatt tcttgtggtactttgtgctgcccttgggtacgcatctcttactggagacctcagttcactggagtctgtcgttt- gctctatactcacttgtagcga ttagggtcttcctgaccgactgatggctcaccgagggattcagcggtatgattgcatcacaccacttcatccct- atagagtcaagtcctaag gtatacccataaagagcctctaatggtctatcctaaggtctatacctaaagataggccatcctatcagtgtcac- ctaaagagggtcttagag agggcctatggagttcctatagggtcctttaaaatataccataaaaatctgagtgactatctcacagtgtacgg- acctaaagttcccccata gggggtacctaaagcccagccaatcacctaaagtcaaccttcggttgaccttgagggttccctaagggttgggg- atgacccttgggtttgt ctttgggtgttaccttgagtgtctctctgtgtccct

TABLE-US-00009 TABLE 9 Sequence of pSL-LuxABE10 (SEQ ID NO: 3). Sequences encoding luciferase subunits are shown in underline. gggcggcgacctcgcgggttttcgctatttatgaaaattttccggtttaaggcgtttccgttcttcttcgtcat- aacttaatgtttttatttaaaata ccctctgaaaagaaaggaaacgacaggtgctgaaagcgaggctttttggcctctgtcgtttcctttctctgttt- ttgtccgtggaatgaacaa tggaagtcaacaaaaagcagctggctgacattttcggtgcgagtatccgtaccattcagaactggcaggaacag- ggaatgcccgttctg cgaggcggtggcaagggtaatgaggtgctttatgactctgccgccgtcataaaatggtatgccgaaagggatgc- tgaaattgagaacga aaagctgcgccgggaggttgaagaactgcggcaggccagcgaggcagatctccagccaggaactattgagtacg- aacgccatcgac ttacgcgtgcgcaggccgacgcacaggaactgaagaatgccagagactccgctgaagtggtggaaaccgcattc- tgtactttcgtgctg tcgcggatcgcaggtgaaattgccagtattctcgacgggctccccctgtcggtgcagcggcgttttccggaact- ggaaaaccgacatgtt gatttcctgaaacgggatatcatcaaagccatgaacaaagcagccgcgctggatgaactgataccggggttgct- gagtgaatatatcga acagtcaggttaacaggctgcggcattttgtccgcgccgggcttcgctcactgttcaggccggagccacagacc- gccgttgaatgggc ggatgctaattactatctcccgaaagaatccgcataccaggaagggcgctgggaaacactgccctttcagcggg- ccatcatgaatgcga tgggcagcgactacatccgtgaggtgaatgtggtgaagtctgcccgtgtcggttattccaaaatgctgctgggt- gtttatgcctactttatag agcataagcagcgcaacacccttatctggttgccgacggatggtgatgccgagaactttatgaaaacccacgtt- gagccgactattcgtg atattccgtcgctgctggcgctggccccgtggtatggcaaaaagcaccgggataacacgctcaccatgaagcgt- ttcactaatgggcgt ggcttctggtgcctgggcggtaaagcggcaaaaaactaccgtgaaaagtcggtggatgtggcgggttatgatga- acttgctgcttttgat gatgatattgaacaggaaggctctccgacgttcctgggtgacaagcgtattgaaggctcggtctggccaaagtc- catccgtggctccac gccaaaagtgagaggcacctgtcagattgagcgtgcagccagtgaatccccgcattttatgcgttttcatgttg- cctgcccgcattgcggg gaggagcagtatcttaaatttggcgacaaagagacgccgtttggcctcaaatggacgccggatgacccctccag- cgtgttttatctctgc gagcataatgcctgcgtcatccgccagcaggagctggactttactgatgcccgttatatctgcgaaaagaccgg- gatctggacccgtgat ggcattctctggttttcgtcatccggtgaagagattgagccacctgacagtgtgacctttcacatctggacagc- gtacagcccgttcaccac ctgggtgcagattgtcaaagactggatgaaaacgaaaggggatacgggaaaacgtaaaaccttcgtaaacacca- cgctcggtgagac gtgggaggcgaaaattggcgaacgtccggatgctgaagtgatggcagagcggaaagagcattattcagcgcccg- ttcctgaccgtgtg gcttacctgaccgccggtatcgactcccagctggaccgctacgaaatgcgcgtatggggatgggggccgggtga- ggaaagctggctg attgaccggcagattattatgggccgccacgacgatgaacagacgctgctgcgtgtggatgaggccatcaataa- aacctatacccgccg gaatggtgcagaaatgtcgatatcccgtatctgctgggatactggcgggattgacccgaccattgtgtatgaac- gctcgaaaaaacatgg gctgttccgggtgatccccattaaaggggcatccgtctacggaaagccggtggccagcatgccacgtaagcgaa- acaaaaacggggtt taccttaccgaaatcggtacggataccgcgaaagagcagatttataaccgcttcacactgacgccggaagggga- tgaaccgcttcccg gtgccgttcacttcccgaataacccggatatttttgatctgaccgaagcgcagcagctgactgctgaagagcag- gtcgaaaaatgggtgg atggcaggaaaaaaatactgtgggacagcaaaaagcgacgcaatgaggcactcgactgcttcgtttatgcgctg- gcggcgctgcgcat cagtatttcccgctggcagctggatctcagtgcgctgctggcgagcctgcaggaagaggatggtgcagcaacca- acaagaaaacactg gcagattacgcccgtgccttatccggagaggatgaatgacgcgacaggaagaacttgccgctgcccgtgcggca- ctgcatgacctgat gacaggtaaacgggtggcaacagtacagaaagacggacgaagggtggagtttacggccacttccgtgtctgacc- tgaaaaaatatatt gcagagctggaagtgcagaccggcatgacacagcgacgcaggggacctgcaggattttatgtatgaaaacgccc- accattcccaccct tctggggccggacggcatgacatcgctgcgcgaatatgccggttatcacggcggtggcagcggatttggagggc- agttgcggtcgtg gaacccaccgagtgaaagtgtggatgcagccctgttgcccaactttacccgtggcaatgcccgcgcagacgatc- tggtacgcaataac ggctatgccgccaacgccatccagctgcatcaggatcatatcgtcgggtcttttttccggctcagtcatcgccc- aagctggcgctatctgg gcatcggggaggaagaagcccgtgccttttcccgcgaggttgaagcggcatggaaagagtttgccgaggatgac- tgctgctgcattga cgttgagcgaaaacgcacgtttaccatgatgattcgggaaggtgtggccatgcacgcctttaacggtgaactgt- tcgttcaggccacctg ggataccagttcgtcgcggcttttccggacacagttccggatggtcagcccgaagcgcatcagcaacccgaaca- ataccggcgacagc cggaactgccgtgccggtgtgcagattaatgacagcggtgcggcgctgggatattacgtcagcgaggacgggta- tcctggctggatgc cgcagaaatggacatggataccccgtgagttacccggcgggcgcgcctcgttcattcacgtttttgaacccgtg- gaggacgggcagact cgcggtgcaaatgtgttttacagcgtgatggagcagatgaagatgctcgacacgctgcagaacacgcagctgca- gagcgccattgtga aggcgatgtatgccgccaccattgagagtgagctggatacgcagtcagcgatggattttattctgggcgcgaac- agtcaggagcagcg ggaaaggctgaccggctggattggtgaaattgccgcgtattacgccgcagcgccggtccggctgggaggcgcaa- aagtaccgcacct gatgccgggtgactcactgaacctgcagacggctcaggatacggataacggctactccgtgtttgagcagtcac- tgctgcggtatatcg ctgccgggctgggtgtctcgtatgagcagctttcccggaattacgcccagatgagctactccacggcacgggcc- agtgcgaacgagtc gtgggcgtactttatggggcggcgaaaattcgtcgcatcccgtcaggcgagccagatgtttctgtgctggctgg- aagaggccatcgttcg ccgcgtggtgacgttaccttcaaaagcgcgcttcagttttcaggaagcccgcagtgcctgggggaactgcgact- ggataggctccggtc gtatggccatcgatggtctgaaagaagttcaggaagcggtgatgctgatagaagccggactgagtacctacgag- aaagagtgcgcaaa acgcggtgacgactatcaggaaatttttgcccagcaggtccgtgaaacgatggagcgccgtgcagccggtctta- aaccgcccgcctgg gcggctgcagcatttgaatccgggctgcgacaatcaacagaggaggagaagagtgacagcagagctgcgtaatc- tcccgcatattgcc agcatggcctttaatgagccgctgatgcttgaacccgcctatgcgcgggttttcttttgtgcgcttgcaggcca- gcttgggatcagcagcct gacggatgcggtgtccggcgacagcctgactgcccaggaggcactcgcgacgctggcattatccggtgatgatg- acggaccacgac aggcccgcagttatcaggtcatgaacggcatcgccgtgctgccggtgtccggcacgctggtcagccggacgcgg- gcgctgcagccgt actcggggatgaccggttacaacggcattatcgcccgtctgcaacaggctgccagcgatccgatggtggacggc- attctgctcgatatg gacacgcccggcgggatggtggcgggggcatttgactgcgctgacatcatcgcccgtgtgcgtgacataaaacc- ggtatgggcgcttg ccaacgacatgaactgcagtgcaggtcagttgcttgccagtgccgcctcccggcgtctggtcacgcagaccgcc- cggacaggctccat cggcgtcatgatggctcacagtaattacggtgctgcgctggagaaacagggtgtggaaatcacgctgatttaca- gcggcagccataag gtggatggcaacccctacagccatcttccggatgacgtccgggagacactgcagtcccggatggacgcaacccg- ccagatgtttgcgc agaaggtgtcggcatataccggcctgtccgtgcaggttgtgctggataccgaggctgcagtgtacagcggtcag- gaggccattgatgc cggactggctgatgaacttgttaacagcaccgatgcgatcaccgtcatgcgtgatgcactggatgcacgtaaat- cccgtctctcaggagg gcgaatgaccaaagagactcaatcaacaactgtttcagccactgcttcgcaggctgacgttactgacgtggtgc- cagcgacggagggc gagaacgccagcgcggcgcagccggacgtgaacgcgcagatcaccgcagcggttgcggcagaaaacagccgcat- tatggggatcc tcaactgtgaggaggctcacggacgcgaagaacaggcacgcgtgctggcagaaacccccggtatgaccgtgaaa- acggcccgccg cattctggccgcagcaccacagagtgcacaggcgcgcagtgacactgcgctggatcgtctgatgcagggggcac- cggcaccgctgg ctgcaggtaacccggcatctgatgccgttaacgatttgctgaacacaccagtgtaagggatgtttatgacgagc- aaagaaacctttaccca ttaccagccgcagggcaacagtgacccggctcataccgcaaccgcgcccctagaccttcatcactaaaggccgc- ctgtgcggcttttttt acgggatttttttatgtcgatgtacacaaccgcccaactgctggcggcaaatgagcagaaatttaagtttgatc- cgctgtttctgcgtctctttt tccgtgagagctatcccttcaccacggagaaagtctatctctcacaaattccgggactggtaaacatggcgctg- tacgtttcgccgattgtt tccggtgaggttatccgttcccgtggcggctccacctctgaatttacgccgggatatgtcaagccgaagcatga- agtgaatccgcagatg accctgcgtcgcctgccggatgaagatccgcagaatctggcggacccggcttaccgccgccgtcgcatcatcat- gcagaacatgcgtg acgaagagctggccattgctcaggtcgaagagatgcaggcagtttctgccgtgcttaagggcaaatacaccatg- accggtgaagccttc gatccggttgaggtggatatgggccgcagtgaggagaataacatcacgcagtccggcggcacggagtggagcaa- gcgtgacaagtc cacgtatgacccgaccgacgatatcgaagcctacgcgctgaacgccagcggtgtggtgaatatcatcgtgttcg- atccgaaaggctgg gcgctgttccgttccttcaaagccgtcaaggagaagctggatacccgtcgtggctctaattccgagctggagac- agcggtgaaagacct gggcaaagcggtgtcctataaggggatgtatggcgatgtggccatcgtcgtgtattccggacagtacgtggaaa- acggcgtcaaaaag aacttcctgccggacaacacgatggtgctggggaacactcaggcacgcggtctgcgcacctatggctgcattca- ggatgcggacgcac agcgcgaaggcattaacgcctctgcccgttacccgaaaaactgggtgaccaccggcgatccggcgcgtgagttc- accatgattcagtc agcaccgctgatgctgctggctgaccctgatgagttcgtgtccgtacaactggcgtaatcatggcccttcgggg- ccattgtttctctgtgga ggagtccatgacgaaagatgaactgattgcccgtctccgctcgctgggtgaacaactgaaccgtgatgtcagcc- tgacggggacgaaa gaagaactggcgctccgtgtggcagagctgaaagaggagcttgatgacacggatgaaactgccggtcaggacac- ccctctcagccgg gaaaatgtgctgaccggacatgaaaatgaggtgggatcagcgcagccggataccgtgattctggatacgtctga- actggtcacggtcgt ggcactggtgaagctgcatactgatgcacttcacgccacgcgggatgaacctgtggcatttgtgctgccgggaa-

cggcgtttcgtgtctc tgccggtgtggcagccgaaatgacagagcgcggcctggccagaatgcaataacgggaggcgctgtggctgattt- cgataacctgttcg atgctgccattgcccgcgccgatgaaacgatacgcgggtacatgggaacgtcagccaccattacatccggtgag- cagtcaggtgcggt gatacgtggtgtttttgatgaccctgaaaatatcagctatgccggacagggcgtgcgcgttgaaggctccagcc- cgtccctgtttgtccgg actgatgaggtgcggcagctgcggcgtggagacacgctgaccatcggtgaggaaaatttctgggtagatcgggt- ttcgccggatgatg gcggaagttgtcatctctggcttggacggggcgtaccgcctgccgttaaccgtcgccgctgaaagggggatgta- tggccataaaaggtc ttgagcaggccgttgaaaacctcagccgtatcagcaaaacggcggtgcctggtgccgccgcaatggccattaac- cgcgttgcttcatcc gcgatatcgcagtcggcgtcacaggttgcccgtgagacaaaggtacgccggaaactggtaaaggaaagggccag- gctgaaaagggc cacggtcaaaaatccgcaggccagaatcaaagttaaccggggggatttgcccgtaatcaagctgggtaatgcgc- gggttgtcctttcgc gccgcaggcgtcgtaaaaaggggcagcgttcatccctgaaaggtggcggcagcgtgcttgtggtgggtaaccgt- cgtattcccggcgc gtttattcagcaactgaaaaatggccggtggcatgtcatgcagcgtgtggctgggaaaaaccgttaccccattg- atgtggtgaaaatccc gatggcggtgccgctgaccacggcgtttaaacaaaatattgagcggatacggcgtgaacgtcttccgaaagagc- tgggctatgcgctg cagcatcaactgaggatggtaataaagcgatgaaacatactgaactccgtgcagccgtactggatgcactggag- aagcatgacaccgg ggcgacgttttttgatggtcgccccgctgtttttgatgaggcggattttccggcagttgccgtttatctcaccg- gcgctgaatacacgggcg aagagctggacagcgatacctggcaggcggagctgcatatcgaagttttcctgcctgctcaggtgccggattca- gagctggatgcgtgg atggagtcccggatttatccggtgatgagcgatatcccggcactgtcagatttgatcaccagtatggtggccag- cggctatgactaccgg cgcgacgatgatgcgggcttgtggagttcagccgatctgacttatgtcattacctatgaaatgtgaggacgcta- tgcctgtaccaaatccta caatgccggtgaaaggtgccgggaccaccctgtgggtttataaggggagcggtgacccttacgcgaatccgctt- tcagacgttgactgg tcgcgtctggcaaaagttaaagacctgacgcccggcgaactgaccgctgagtcctatgacgacagctatctcga- tgatgaagatgcaga ctggactgcgaccgggcaggggcagaaatctgccggagataccagcttcacgctggcgtggatgcccggagagc- aggggcagcag gcgctgctggcgtggtttaatgaaggcgatacccgtgcctataaaatccgcttcccgaacggcacggtcgatgt- gttccgtggctgggtc agcagtatcggtaaggcggtgacggcgaaggaagtgatcacccgcacggtgaaagtcaccaatgtgggacgtcc- gtcgatggcaga agatcgcagcacggtaacagcggcaaccggcatgaccgtgacgcctgccagcacctcggtggtgaaagggcaga- gcaccacgctg accgtggccttccagccggagggcgtaaccgacaagagctttcgtgcggtgtctgcggataaaacaaaagccac- cgtgtcggtcagtg gtatgaccatcaccgtgaacggcgttgctgcaggcaaggtcaacattccggttgtatccggtaatggtgagttt- gctgcggttgcagaaat taccgtcaccgccagttaatccggagagtcagcgatgttcctgaaaaccgaatcatttgaacataacggtgtga- ccgtcacgctttctgaa ctgtcagccctgcagcgcattgagcatctcgccctgatgaaacggcaggcagaacaggcggagtcagacagcaa- ccggaagtttact gtggaagacgccatcagaaccggcgcgtttctggtggcgatgtccctgtggcataaccatccgcagaagacgca- gatgccgtccatga atgaagccgttaaacagattgagcaggaagtgcttaccacctggcccacggaggcaatttctcatgctgaaaac- gtggtgtaccggctgt ctggtatgtatgagtttgtggtgaataatgcccctgaacagacagaggacgccgggcccgcagagcctgtttct- gcgggaaagtgttcg acggtgagctgagttttgccctgaaactggcgcgtgagatggggcgacccgactggcgtgccatgcttgccggg- atgtcatccacgga gtatgccgactggcaccgcttttacagtacccattattttcatgatgttctgctggatatgcacttttccgggc- tgacgtacaccgtgctcagc ctgtttttcagcgatccggatatgcatccgctggatttcagtctgctgaaccggcgcgaggctgacgaagagcc- tgaagatgatgtgctg atgcagaaagcggcagggcttgccggaggtgtccgctttggcccggacgggaatgaagttatccccgcttcccc- ggatgtggcggac atgacggaggatgacgtaatgctgatgacagtatcagaagggatcgcaggaggagtccggtatggctgaaccgg- taggcgatctggtc gttgatttgagtctggatgcggccagatttgacgagcagatggccagagtcaggcgtcatttttctggtacgga- aagtgatgcgaaaaaa acagcggcagtcgttgaacagtcgctgagccgacaggcgctggctgcacagaaagcggggatttccgtcgggca- gtataaagccgc catgcgtatgctgcctgcacagttcaccgacgtggccacgcagcttgcaggcgggcaaagtccgtggctgatcc- tgctgcaacagggg gggcaggtgaaggactccttcggcgggatgatccccatgttcagggggcttgccggtgcgatcaccctgccgat- ggtgggggccacc tcgctggcggtggcgaccggtgcgctggcgtatgcctggtatcagggcaactcaaccctgtccgatttcaacaa- aacgctggtcctttcc ggcaatcaggcgggactgacggcagatcgtatgctggtcctgtccagagccgggcaggcggcagggctgacgtt- taaccagaccag cgagtcactcagcgcactggttaaggcgggggtaagcggtgaggctcagattgcgtccatcagccagagtgtgg- cgcgtttctcctctg catccggcgtggaggtggacaaggtcgctgaagccttcgggaagctgaccacagacccgacgtcggggctgacg- gcgatggctcgc cagttccataacgtgtcggcggagcagattgcgtatgttgctcagttgcagcgttccggcgatgaagccggggc- attgcaggcggcga acgaggccgcaacgaaagggtttgatgaccagacccgccgcctgaaagagaacatgggcacgctggagacctgg- gcagacaggac tgcgcgggcattcaaatccatgtgggatgcggtgctggatattggtcgtcctgataccgcgcaggagatgctga- ttaaggcagaggctg cgtataagaaagcagacgacatctggaatctgcgcaaggatgattattttgttaacgatgaagcgcgggcgcgt- tactgggatgatcgtg aaaaggcccgtcttgcgcttgaagccgcccgaaagaaggctgagcagcagactcaacaggacaaaaatgcgcag- cagcagagcgat accgaagcgtcacggctgaaatataccgaagaggcgcagaaggcttacgaacggctgcagacgccgctggagaa- atataccgcccg tcaggaagaactgaacaaggcactgaaagacgggaaaatcctgcaggcggattacaacacgctgatggcggcgg- cgaaaaaggatt atgaagcgacgctgaaaaagccgaaacagtccagcgtgaaggtgtctgcgggcgatcgtcaggaagacagtgct- catgctgccctgc tgacgcttcaggcagaactccggacgctggagaagcatgccggagcaaatgagaaaatcagccagcagcgccgg- gatttgtggaag gcggagagtcagttcgcggtactggaggaggcggcgcaacgtcgccagctgtctgcacaggagaaatccctgct- ggcgcataaagat gagacgctggagtacaaacgccagctggctgcacttggcgacaaggttacgtatcaggagcgcctgaacgcgct- ggcgcagcaggc ggataaattcgcacagcagcaacgggcaaaacgggccgccattgatgcgaaaagccgggggctgactgaccggc- aggcagaacgg gaagccacggaacagcgcctgaaggaacagtatggcgataatccgctggcgctgaataacgtcatgtcagagca- gaaaaagacctgg gcggctgaagaccagcttcgcgggaactggatggcaggcctgaagtccggctggagtgagtgggaagagagcgc- cacggacagta tgtcgcaggtaaaaagtgcagccacgcagacctttgatggtattgcacagaatatggcggcgatgctgaccggc- agtgagcagaactg gcgcagcttcacccgttccgtgctgtccatgatgacagaaattctgcttaagcaggcaatggtggggattgtcg- ggagtatcggcagcgc cattggcggggctgttggtggcggcgcatccgcgtcaggcggtacagccattcaggccgctgcggcgaaattcc- attttgcaaccgga ggatttacgggaaccggcggcaaatatgagccagcggggattgttcaccgtggtgagtttgtcttcacgaagga- ggcaaccagccgga ttggcgtggggaatctttaccggctgatgcgcggctatgccaccggcggttatgtcggtacaccgggcagcatg- gcagacagccggtc gcaggcgtccgggacgtttgagcagaataaccatgtggtgattaacaacgacggcacgaacgggcagataggtc- cggctgctctgaa ggcggtgtatgacatggcccgcaagggtgcccgtgatgaaattcagacacagatgcgtgatggtggcctgttct- ccggaggtggacga tgaagaccttccgctggaaagtgaaacccggtatggatgtggcttcggtcccttctgtaagaaaggtgcgcttt- ggtgatggctattctcag cgagcgcctgccgggctgaatgccaacctgaaaacgtacagcgtgacgctttctgtcccccgtgaggaggccac- ggtactggagtcgt ttctggaagagcacgggggctggaaatcctttctgtggacgccgccttatgagtggcggcagataaaggtgacc- tgcgcaaaatggtcg tcgcgggtcagtatgctgcgtgttgagttcagcgcagagtttgaacaggtggtgaactgatgcaggatatccgg- caggaaacactgaat gaatgcacccgtgcggagcagtcggccagcgtggtgctctgggaaatcgacctgacagaggtcggtggagaacg- ttattttttctgtaat gagcagaacgaaaaaggtgagccggtcacctggcaggggcgacagtatcagccgtatcccattcaggggagcgg- ttttgaactgaat ggcaaaggcaccagtacgcgccccacgctgacggtttctaacctgtacggtatggtcaccgggatggcggaaga- tatgcagagtctgg tcggcggaacggtggtccggcgtaaggtttacgcccgttttctggatgcggtgaacttcgtcaacggaaacagt- tacgccgatccggag caggaggtgatcagccgctggcgcattgagcagtgcagcgaactgagcgcggtgagtgcctcctttgtactgtc- cacgccgacggaaa cggatggcgctgtttttccgggacgtatcatgctggccaacacctgcacctggacctatcgcggtgacgagtgc- ggttatagcggtccg gctgtcgcggatgaatatgaccagccaacgtccgatatcacgaaggataaatgcagcaaatgcctgagcggttg- taagttccgcaataa cgtcggcaactttggcggcttcctttccattaacaaactttcgcagtaaatcccatgacacagacagaatcagc- gattctggcgcacgccc ggcgatgtgcgccagcggagtcgtgcggatcgtggtaagcacgccggagggggaaagatatttcccctgcgtga- atatctccggtga gccggaggctatttccgtatgtcgccggaagactggctgcaggcagaaatgcagggtgagattgtggcgctggt- ccacagccacccc ggtggtctgccctggctgagtgaggccgaccggcggctgcaggtgcagagtgatttgccgtggtggctggtctg- ccgggggacgattc ataagttccgctgtgtgccgcatctcaccgggcggcgctttgagcacggtgtgacggactgttacacactgttc- cgggatgcttatcatct ggcggggattgagatgccggactttcatcgtgaggatgactggtggcgtaacggccagaatctctatctggata- atctggaggcgacgg ggctgtatcaggtgccgttgtcagcggcacagccgggcgatgtgctgctgtgctgttttggttcatcagtgccg- aatcacgccgcaattta ctgcggcgacggcgagctgctgcaccatattcctgaacaactgagcaaacgagagaggtacaccgacaaatggc- agcgacgcacac actccctctggcgtcaccgggcatggcgcgcatctgcctttacggggatttacaacgatttggtcgccgcatcg- accttcgtgtgaaaac

gggggctgaagccatccgggcactggccacacagctcccggcgtttcgtcagaaactgagcgacggctggtatc- aggtacggattgc cgggcgggacgtcagcacgtccgggttaacggcgcagttacatgagactctgcctgatggcgctgtaattcata- ttgttcccagagtcgc cggggccaagtcaggtggcgtattccagattgtcctgggggctgccgccattgccggatcattctttaccgccg- gagccacccttgcag catggggggcagccattggggccggtggtatgaccggcatcctgttttctctcggtgccagtatggtgctcggt- ggtgtggcgcagatg ctggcaccgaaagccagaactccccgtatacagacaacggataacggtaagcagaacacctatttctcctcact- ggataacatggttgc ccagggcaatgttctgcctgttctgtacggggaaatgcgcgtggggtcacgcgtggtttctcaggagatcagca- cggcagacgaaggg gacggtggtcaggttgtggtgattggtcgctgatgcaaaatgttttatgtgaaaccgcctgcgggcggttttgt- catttatggagcgtgagg aatgggtaaaggaagcagtaaggggcataccccgcgcgaagcgaaggacaacctgaagtccacgcagttgctga- gtgtgatcgatgc catcagcgaagggccgattgaaggtccggtggatggcttaaaaagcgtgctgctgaacagtacgccggtgctgg- acactgaggggaa taccaacatatccggtgtcacggtggtgttccgggctggtgagcaggagcagactccgccggagggatttgaat- cctccggctccgag acggtgctgggtacggaagtgaaatatgacacgccgatcacccgcaccattacgtctgcaaacatcgaccgtct- gcgctttaccttcggt gtacaggcactggtggaaaccacctcaaagggtgacaggaatccgtcggaagtccgcctgctggttcagataca- acgtaacggtggct gggtgacggaaaaagacatcaccattaagggcaaaaccacctcgcagtatctggcctcggtggtgatgggtaac- ctgccgccgcgcc cgtttaatatccggatgcgcaggatgacgccggacagcaccacagaccagctgcagaacaaaacgctctggtcg- tcatacactgaaat catcgatgtgaaacagtgctacccgaacacggcactggtcggcgtgcaggtggactcggagcagttcggcagcc- agcaggtgagcc gtaattatcatctgcgcgggcgtattctgcaggtgccgtcgaactataacccgcagacgcggcaatacagcggt- atctgggacggaacg tttaaaccggcatacagcaacaacatggcctggtgtctgtgggatatgctgacccatccgcgctacggcatggg- gaaacgtcttggtgc ggcggatgtggataaatgggcgctgtatgtcatcggccagtactgcgaccagtcagtgccggacggctttggcg- gcacggagccgcg catcacctgtaatgcgtacctgaccacacagcgtaaggcgtgggatgtgctcagcgatttctgctcggcgatgc- gctgtatgccggtatg gaacgggcagacgctgacgttcgtgcaggaccgaccgtcggataagacgtggacctataaccgcagtaatgtgg- tgatgccggatgat ggcgcgccgttccgctacagcttcagcgccctgaaggaccgccataatgccgttgaggtgaactggattgaccc- gaacaacggctgg gagacggcgacagagcttgttgaagatacgcaggccattgcccgttacggtcgtaatgttacgaagatggatgc- ctttggctgtaccagc cgggggcaggcacaccgcgccgggctgtggctgattaaaacagaactgctggaaacgcagaccgtggatttcag- cgtcggcgcaga agggcttcgccatgtaccgggcgatgttattgaaatctgcgatgatgactatgccggtatcagcaccggtggtc- gtgtgctggcggtgaa cagccagacccggacgctgacgctcgaccgtgaaatcacgctgccatcctccggtaccgcgctgataagcctgg- ttgacggaagtgg caatccggtcagcgtggaggttcagtccgtcaccgacggcgtgaaggtaaaagtgagccgtgttcctgacggtg- ttgctgaatacagcg tatgggagctgaagctgccgacgctgcgccagcgactgttccgctgcgtgagtatccgtgagaacgacgacggc- acgtatgccatcac cgccgtgcagcatgtgccggaaaaagaggccatcgtggataacggggcgcactttgacggcgaacagagtggca- cggtgaatggtg tcacgccgccagcggtgcagcacctgaccgcagaagtcactgcagacagcggggaatatcaggtgctggcgcga- tgggacacacc gaaggtggtgaagggcgtgagtttcctgctccgtctgaccgtaacagcggacgacggcagtgagcggctggtca- gcacggcccgga cgacggaaaccacataccgcttcacgcaactggcgctggggaactacaggctgacagtccgggcggtaaatgcg- tgggggcagcag ggcgatccggcgtcggtatcgttccggattgccgcaccggcagcaccgtcgaggattgagctgacgccgggcta- ttttcagataaccg ccacgccgcatcttgccgtttatgacccgacggtacagtttgagttctggttctcggaaaagcagattgcggat- atcagacaggttgaaac cagcacgcgttatcttggtacggcgctgtactggatagccgccagtatcaatatcaaaccgggccatgattatt- acttttatatccgcagtgt gaacaccgttggcaaatcggcattcgtggaggccgtcggtcgggcgagcgatgatgcggaaggttacctggatt- ttttcaaaggcaaga taaccgaatcccatctcggcaaggagctgctggaaaaagtcgagctgacggaggataacgccagcagactggag- gagttttcgaaag agtggaaggatgccagtgataagtggaatgccatgtgggctgtcaaaattgagcagaccaaagacggcaaacat- tatgtcgcgggtatt ggcctcagcatggaggacacggaggaaggcaaactgagccagtttctggttgccgccaatcgtatcgcatttat- tgacccggcaaacg ggaatgaaacgccgatgtttgtggcgcagggcaaccagatattcatgaacgacgtgttcctgaagcgcctgacg- gcccccaccattacc agcggcggcaatcctccggccttttccctgacaccggacggaaagctgaccgctaaaaatgcggatatcagtgg- cagtgtgaatgcga actccgggacgctcagtaatgtgacgatagctgaaaactgtacgataaacggtacgctgagggcggaaaaaatc- gtcggggacattgt aaaggcggcgagcgcggcttttccgcgccagcgtgaaagcagtgtggactggccgtcaggtacccgtactgtca- ccgtgaccgatga ccatccttttgatcgccagatagtggtgcttccgctgacgtttcgcggaagtaagcgtactgtcagcggcagga- caacgtattcgatgtgtt atctgaaagtactgatgaacggtgcggtgatttatgatggcgcggcgaacgaggcggtacaggtgttctcccgt- attgttgacatgccag cgggtcggggaaacgtgatcctgacgttcacgcttacgtccacacggcattcggcagatattccgccgtatacg- tttgccagcgatgtgc aggttatggtgattaagaaacaggcgctgggcatcagcgtggtctgagtgtgttacagaggttcgtccgggaac- gggcgttttattataaa acagtgagaggtgaacgatgcgtaatgtgtgtattgccgttgctgtctttgccgcacttgcggtgacagtcact- ccggcccgtgcggaag gtggacatggtacgtttacggtgggctattttcaagtgaaaccgggtacattgccgtcgttgtcgggcggggat- accggtgtgagtcatct gaaagggattaacgtgaagtaccgttatgagctgacggacagtgtgggggtgatggcttccctggggttcgccg- cgtcgaaaaagagc agcacagtgatgaccggggaggatacgtttcactatgagagcctgcgtggacgttatgtgagcgtgatggccgg- accggttttacaaat cagtaagcaggtcagtgcgtacctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatt- aatgtgagttagct cactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataac- aatttcacacaggaaaca gctatgaccatgattacgccaagctatttaggtgacactatagaatactcaagctatgcatccaacgcgttggg- agctctcccatatggtcg acctgcaggcggccgcgaattcactagtgattatggtaaagcaagatgaagttatcacattgttatcaaatatt- cgtagtaatcgatgcaag atatattctttgttaggaagttcgcatgacttgagtgaaaatttagtggtcctgcgcaatttttatcaatcggt- tacgaaagccgctatcgcgat ggataatgatcatctggatattgatgttgatattactgaaccgtcatttgaacatttaactattgcgacagtca- atgaacgccgaatgagaatt gagattgaaaatcaagcaatttctctgtcttaaaatctattgagatattctatcactcaaatagcaatataagg- actctctatgaaatttggaaa ctttttgcttacataccaacctccccaattttctcaaacagaggtaatgaaacgtttggttaaattaggtcgca- tctctgaggagtgtggttttg ataccgtatggttactggagcatcatttcacggagtttggtttgcttggtaacccttatgtcgctgctgcatat- ttacttggcgcgactaaaaaa ttgaatgtaggaactgccgctattgttcttcccacagcccatccagtacgccaacttgaagatgtgaatttatt- ggatcaaatgtcaaaagga cgatttcggtttggtatttgccgagggctttacaacaaggactttcgcgtattcggcacagatatgaataacag- tcgcgccttagcggaatg ctggtacgggctgataaagaatggcatgacagagggatatatggaagctgataatgaacatatcaagttccata- aggtaaaagtaaaccc cgcggcgtatagcagaggtggcgcaccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctc- aatttggcctaccgat gatattaagttggattataaatactaacgaaaagaaagcacaacttgagctttataatgaagtggctcaagaat- atgggcacgatattcata atatcgaccattgcttatcatatataacatctgtagatcatgactcaattaaagcgaaagagatttgccggaaa- tttctggggcattggtatga ttcttatgtgaatgctacgactatttttgatgattcagaccaaacaagaggttatgatttcaataaagggcagt- ggcgtgactttgtattaaaag gacataaagatactaatcgccgtattgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtatt- gacataattcaaaaaga cattgatgctacaggaatatcaaatatttgttgtggatttgaagctaatggaacagtagacgaaattattgctt- ccatgaagctcttccagtct gatgtcatgccatttcttaaagaaaaacaacgttcgctattatattagctaaggagaaagaaatgaaatttgga- ttgttcttccttaacttcatc aattcaacaactgttcaagaacaaagtatagttcgcatgcaggaaataacggagtatgttgataagttgaattt- tgaacagattttagtgtatg aaaatcatttttcagataatggtgttgtcggcgctcctctgactgtttctggttttctgctcggtttaacagag- aaaattaaaattggttcattaaa tcacatcattacaactcatcatcctgtcgccatagcggaggaagcttgcttattggatcagttaagtgaaggga- gatttattttagggtttagt gattgcgaaaaaaaagatgaaatgcatttttttaatcgcccggttgaatatcaacagcaactatttgaagagtg- ttatgaaatcattaacgatg ctttaacaacaggctattgtaatccagataacgatttttatagcttccctaaaatatctgtaaatccccatgct- tatacgccaggcggacctcg gaaatatgtaacagcaaccagtcatcatattgttgagtgggcggccaaaaaaggtattcctctcatctttaagt- gggatgattctaatgatgtt agatatgaatatgctgaaagatataaagccgttgcggataaatatgacgttgacctatcagagatagaccatca- gttaatgatattagttaac tataacgaagatagtaataaagctaaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccc- taatgaaaatttcgaaaat aaacttgaagaaataattgcagaaaacgctgtcggaaattatacggagtgtataactgcggctaagttggcaat- tgaaaagtgtggtgcg aaaagtgtattgctgtcctttgaaccaatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatga- taatattaagaagtaccacat ggaatatacctaatagatttcgagttgcagcgaggcggcaagtgaacgaatccccaggagcatagataactatg- tgactggggtgagtg aaagcagccaacaaagcagcagcttgaaagatgaagggtataaaagagtatgacagcagtgctgccatactttc- taatattatcttgagg agtaaaacaggtatgacttcatatgttgataaacaagaaattacagcaagctcagaaattgatgatttgatttt- ttcgagcgatccattagtgt ggtcttacgacgagcaggaaaaaatcagaaagaaacttgtgcttgatgcatttcgtaatcattataaacattgt- cgagaatatcgtcactact gtcaggcacacaaagtagatgacaatattacggaaattgatgacatacctgtattcccaacatcggtttttaag- tttactcgcttattaacttct

caggaaaacgagattgaaagttggtttaccagtagcggcacgaatggtttaaaaagtcaggtggcgcgtgacag- attaagtattgagag actcttaggctctgtgagttatggcatgaaatatgttggtagttggtttgatcatcaaatagaattagtcaatt- tgggaccagatagatttaatg ctcataatatttggtttaaatatgttatgagtttggtggaattgttatatcctacgacatttaccgtaacagaa- gaacgaatagattttgttaaaac attgaatagtcttgaacgaataaaaaatcaagggaaagatctttgtcttattggttcgccatactttatttatt- tactctgccattatatgaaagat aaaaaaatctcattttctggagataaaagcctttatatcataaccggaggcggctggaaaagttacgaaaaaga- atctctgaaacgtgatg atttcaatcatcttttatttgatactttcaatctcagtgatattagtcagatccgagatatatttaatcaagtt- gaactcaacacttgtttctttgagg atgaaatgcagcgtaaacatgttccgccgtgggtatatgcgcgagcgcttgatcctgaaacgttgaaacctgta- cctgatggaacgccgg ggttgatgagttatatggatgcgtcagcaaccagttatccagcatttattgttaccgatgatgtcgggataatt- agcagagaatatggtaagt atcccggcgtgctcgttgaaattttacgtcgcgtcaatacgaggacgcagaaagggtgtgctttaagcttaaac- caagcatttaatagttga cataatcgaattcccgcggccgccatggcggccgggagcatgcgacgtcgggcccaattcgccctatagtgagt- cgtattacaattcac tggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccc- cctttcgccaggtacgc catggccggagtggctcacagtcggtggtccggcagtacaatggattaccgtaagacggaaatcactcccggga- tcctctagagtcga cctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaatt- ccacacaacatacgagcc ggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcc- cgctttccagtcggga aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttc- cgcttcctcgctcact gactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccac- agaatcaggggataa cgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttt- tccataggctccg cccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacc- aggcgtttccccct ggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggg- aagcgtggcgctttctca tagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccg- ttcagcccgaccgctg cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg- gtaacaggattagcag agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtat- ttggtatctgcgctctg ctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgg- tttttttgtttgcaagca gcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtgga- acgaaaactcacgttaa gggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatc- aatctaaagtatatatgagtaa acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccat- agttgcctgactccccgtcgt gtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcac- cggctccagatttat cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtct- attaattgttgccgg gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtc- acgctcgtcgtttggtatg gcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttag- ctccttcggtcctccgat cgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtca- tgccatccgtaagatgctttt ctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcg- tcaatacgggataatac cgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct- taccgctgttgagatcc agttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagc- aaaaacaggaaggcaaaa tgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaa- gcatttatcagggttattgt ctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaa- agtgccacctgacgtcta agaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcg- gtgatgacggtgaaaacc tctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcag- ggcgcgtcagcg ggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcg- gtgtgaaataccgca cagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgat- cggtgcgggcctct tcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttccca- gtcacgacgttgtaaa acgacggccagtgaattcgattttaagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaa- aatgctttatttgtgaaat ttgtgatgctattgattatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattt- tatgtttcaggttcaggggga ggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcagttatctagag- tcgcggccgctttacttgta cagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgt- tggggtctttgctca gggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctgg- tagtggtcggcga gctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggcc- atgatatagacgttgtggct gttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgc- ggttcaccagggtgtcgc cctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtag- ccttcgggcatggcgg acttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtc- acgagggtgggcca gggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccct- cgccggacacgct gaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgccct- tgctcaccatggtgg cgaccggtggatcgatcctagcggatctgacggttcactaaaccagctctgcttatatagacctcccaccgtac- acgcctaccgcccattt gcgtcaatggggcggagttgttacgacattttggaaagtcccgttgattttggtgccaaaacaaactcccattg- acgtcaatggggtggag acttggaaatccccgtgagtcaaaccgctatccacgcccattgatgtactgccaaaaccgcatcaccatggtaa- tagcgatgactaatac gtagatgtactgccaagtaggaaagtcccataaggtcatgtactgggcataatgccaggcgggccatttaccgt- cattgacgtcaatagg gggcgtacttggcatatgatacacttgatgtactgccaagtgggcagtttaccgtaaatactccacccattgac- gtcaatggaaagtcccta ttggcgttactatgggaacatacgtcattattgacgtcaatgggcgggggtcgttgggcggtcagccaggcggg- ccatttaccgtaagtt atgtaacgcggaactccatatatgggctatgaactaatgaccccgtaattgattactattaaatcactagtgaa- ttcgattaaagcgacggc acagctcgcggaaaatatcaaagtcgttgcgcgcctcgaactgcggcggcaccacctgtttcatggcgataatg- ccacggttggagtgg ttgccgtactggtcgagatcgttacgctcaaactgcgtggtcgcaggcagcacgatatcggcaaagcggcaggt- tgaggtccactggtt atctatggcgataaccgtttccagcttgcgccagccttcaataatgcggttgatctgctgatggcgatggaatg- ggttagttccggcaaaaa tacacattttcagcggcggcagttttaccgatttaccgttccagttgatcactttccccggttcgaggatcgca- tcgataaaacgggcaatc ggaatggtgctgctgtagcctttgtaatcactgttgtcgtgaacaggcggaatcgacgtagagccggagaaacc- actcagaataacgcct ttacgccccggcgtgcctgcgccgttatagtgccagccaaaaccaaagccaccacctggcaggccaatttgccc- cagcatcgccgcca gaaccacaatcatccacgcccactgttcaccgtgctgcatacgctgcacgcaccagccagcaataatttgcgtt- ctgttcgccgccatctg ccgcgccagcccacgaatggtttcggcatcaatgccggtcagtttttcagcccatgcggcatctttcggctgac- cgtctttctcacccagc aggtacggcaggaactgctcaaaacccacacagtagttagcgaggaagtttttgtcgtacaggttttcactgta- cagcgtatacgccagc gccagttgcagcggcacatcagtttgcgggttaaccgcaatgtgcttcacatgctcgcgccccagatactcatg- ggtggatgtgacaacc ggatcgatgctgatgacctcaatttcaccggcggtgactttcgcttttagctgcgcgtaatattcataaacatc- gtgatccgggcaccacca gttcgcttgctggtttttcagcaaatcagagccccacagcacaatggttttgctgttctgcaataccagcggcc- aggaggtttgctgttcata cacttccattgagccaaccacgcgcggcaggatcacctgcgcagcaccggtagagtaatctccgcccgtaccaa- cgctattaccatgca aggcaatagctttcgccagcatccccgaagcgttatggaacatccccgtcgattgccaaccactggcggtcagc- aaggcactcggccc gtgagttttctgcacgcgttccagttcttcatagaacatgtcgagggcttcatcccagctcacgcgcacaaaac- ggttatcaccgcgctgg gaggtatcgctgagatggcgcttacgcagccagtccacgcgtaccatcggataacgaatacgcgccgcgttgtg- tacgtgatccggca atccggcaatcattttcgacggatatttatccagttcgaacggttttgccgccacaaagcgaccatccttcacc- gtcgcgcggatagcccc ccagtgcgacccggtaagaatgccctctttcgagatgacagcctcagtcgccgcttgcgccgcagtcgcacggc- gcggcgttaacaat gacggccccagcatcccggcgacggttaagccgccgagttgtgccagaaaacgccgacgtgatgcctgaaagag- atcgttattgttca ttatttttcttccttcttatcgccgtgagccttacctgcggtgtcagacgcattcatttgcagatatttcaaca- aagtgcgttcttcacgtttatcg agactggtaaagccaatcatgccgttgagcgtgccgatccaaccgttagcgtcaaagtgggcgatttccggtgc- gccgtggcactggtt acaggtgccgttgtacaacgaatccgcataagcccagatcggtttgatatcgttcaccatgtcgcctttcttca- tccacgcagtggcctgca acttgctccactcggtattggtgtcggcaacggtggttttctccagcgtttttacctgctgctgcacatcacca- cgaatcgaggcaacaaag atgcgtttacctgggaattgggtgagtacacgctgacgtccggcgctttccgtccagccggtaatttcaatttg- cagccagtcgccgtcac gtttaaggactttcacttccgaagcaggcagcagagaaccagaggcttctttatcgcctttcgccgcataaatt-

ggcttaatatcaatagagt acagcgtgtcaccactgtcattagcactggcgcgcagctcatcgaactgcttacggaagccgctactcatatcc- ggtaactggtgggcaa tacctttatgacagtcgatgcaggattgattatctttcgctgccaccttcatctgacgtgccgcttcaggatgc- tgcttcgcatgatccatcgc atcgtagttatggcaggagcggcaggttgccgagttgttttctttcattcgcgcccattcacgctcggcaagtt- ccgcgcgtttggcttcga atttttcaggtgtatcaatggagtgagcaataaaggtctggtagatatcattgctcgcttccagtttgcgcttc- accatgcctggaatatccgg cgggatatgacagtcatggcattcagctcgcacgccggaggcgttctggaaatgcaccgactgtttatattctt- catacaccggttgcata ctgtggcaactgacacaaaattcggttgtgctggtgactttgatcccaacgtgtggcaatacaatcagcgcaat- gccaatcacaatcccaa ttgcgaccagcgccagtaccgaccaacgagcactgggtcggcgtagcgcgttccagagtttccgcataatagcc- cctgtaaaattatgg tttagtgaagcgatcttaatgagcaaatatgaacagcggcactggtcaggatgaacggcttacggcagaatatg- aacagatatgaacag aatgagtaaaaccctctgatgccacatcacattgttattgttgaagatgagccggttacccaggcgcgattaca- atcttacttcactcagga ggggtataccgtttccgttacagcgagcggtgccgggctgcgggaaattatgcagaatcagccggtagatttaa- ttctgctggatatcaa cttacccgatgaaaatggcctgatgttaacccgcgccctgcgagaacgctcaacggtggggattattctggtta- ccggacgcagcgatc ggattgaccgtattgttgggctggaaatgggcgcagacgattacgtcaccaaaccgctggaactgcgcgaactg- gtagtacgggtgaa aaatctgctctggcgaatcgacctcgcgcgacaagctcaaccgcacactcaggacaactgctatcgctttgccg- gttattgcctgaatgtg tcgcgccatacgctggagcgggatggcgagccgattaaactgacccgcgcagagtatgaaatgttggtggcatt- tgtgacgaatccgg gcgaaattctcagccgtgaacgtctgctacgtatgctttctgcgcgtcgggtggaaaaccctgacctgcgcacc- gtcgatgtgttaattcgt cgtttacgtcataaactcagcgcggatttactggtgacgcaacatggtgaaggttatttcttagccgctgatgt- gtgctgataaaaatagacc ggacgaaatccccctggtgacagcgagcggcggatatgttctcggtcggcatttttcggcgtcagaactaaaat- cggtgggctgacatta tcagacaccgattgcccctgtaattgcctgatggcctgctcaactgccagttccccctgccagaccatttgatc- gctggcagccataatca ctcttccccgcttcagcccgcgatacacctgatgtgaaagataaaacgacaccacggtaagcggcgttttcagg- ttacgcccttcacccat tgccgcctctgccgcaatggccgttccggcaacgacgtcaatttctgggtggcgttccagcatctcctgcaaca- ggttacgctggatttca atatcgttatcaccaagcgcaatatcaacaatacgcaccgggcttccggcaatggctgcgcgaaaaccctcgac- catctctttactgccc ccggcattatcgggtccgggcatcaacagcacgttcagtggtttaccgtgcgcccattgcaccaaatatcgccc- aggttgatagcccatc tgaaaccagggtacaccaacgcggcttttcacctggggagcatcaatagcatttaccagttcgatcaccggcag- acttgctacctgctttt gcagatcgggaaatgaggtcgtgctactaccgagtaaaatggcctctgcgccccactgtttacactggtcgatt- tgtgcttgctgggtagc caactggctgtagccgcctgcctccagcacttttaaatccacaccatagcggcgagctgcctcctgcataccat- agttcaacgataacca gtatgaatctttcaggctgggataaagcgcgcacagtttccatgcgcgtttggctttaagcggcatagaggctt- gcaccgtgaaatgctgc gcatcatgccagcgcaacaggttatcagccgaaaatgccggcaacatgaaaagggaaagaagtaaaaatagcag- tacgcgcatgata gcctcatcaataataaggctttatgctagatgcattccgctttgcgactcaacctttttcaccttaagtgcacc- gaccgtgaatttaaccctga cccgaagactctggatgggctttgccctgatggcgctgttaaccctgaccagtaccctggtgggatggtacaac- ctgcgctttatcagcc aggtggaaaaagacaacactcaggcattgattcctaccatgaatatggcgcgccagttgagcgaagccagcgcc- tgggaacttttcgcc gcgcagaacctgaccagtgccgataacgaaaagatgtggcaggcgcaggggcgaatgctcaccgcacaaagcct- gaagattaatgc gttgctgcaagcgttacgggaacaaggttttgataccaccgctattgaacaacaggagcaggagatctcccgtt- cattacgtcagcaagg ggaactggtggggcggcgtctgcaactacgccagcaacaacggcaactcagtcagcagatagtcgctgccgccg- atgagatcgcac gcctggcgcaaggtcaggcgaataatgcgacaacttccgctggagcgacccaggccgggatttacgatttgatc- gaacaagatcagcg tcaggctgctgaaagtgcactcgatcggctgattgatatcgatcttgagtatgttaaccagatgaatgaactgc- gccttagcgctctgcgg gtgcagcaaatggtgatgaatctggggctggagcagatccagaaaaatgcaccaacgctggaaaagcagctcaa- taatgcggtgaaa attctgcaacgtcggcaaatacgcattgaagatccgggtgttcgtgcgcaggtcgcaacaacgttaactaccgt- tagccaatatagcgatt tgctggcgctgtatcagcaggacagtgaaatcagcaatcacctacaaactctcgcacaaaataacatcgcccag- ttcgcgcagtttagta gcgaagtcagtcagctggtcgactcggtacccggggatccactcgttattctcggacgagtgttcagtaatgaa- cctctggagagaacca tgtatatgatcgttatctgggttggacttctgatttaagcccagataactggcctgaatatgttaatgagagaa- tcggtattcctcatgtgtgg catgttttcgtctttgctcttgcattttcgctagcaattaatgtgcatcgattatcagctattgccagcgccag- atataagcgatttaagctaaga aaacgcattaagatgcaaaacgataaagtgcgatcagtaattcaaaaccttacagaagagcaatctatggtttt- gtgcgcagcccttaatg aaggcaggaagtatgtggttacatcaaaacaattcccatacattagtgagttgattgagcttggtgtgttgaac- aaaactttttcccgatgga atggaaagcatatattattccctattgaggatatttactggactgaattagttgccagctatgatccatataat- attgagataaagccaaggcc aatatctaagtaactagataagaggaatcgattttccataattttctggcgtccactgcatgttatgccgcgtt- cgccaggcttgctgtaccat gtgcgctgattcttgcgctcaatacgttgcaggttgctttcaatctgtttgtggtattcagccagcactgtaag- gtctatcggatttagtgcgct ttctactcgtgatttcggtttgcgattcagcgagagaatagggcggttaactggttttgcgcttaccccaacca- acaggggatttgctgcttt ccattgagcctgtttctctgcgcgacgttcgcggcggcgtgtttgtgcatccatctggattctcctgtcagtta- gctttggtggtgtgtggcag ttgtagtcctgaacgaaaaccccccgcgattggcacattggcagctaatccggaatcgcacttacggccaatgc- ttcgtttcgtatcacac accccaaagccttctgctttgaatgctgcccttcttcagggcttaatttttaagagcgtcaccttcatggtggt- cagtgcgtcctgctgatgtg ctcagtatcaccgccagtggtatttatgtcaacaccgccagagataatttatcaccgcagatggttatctgtat- gttttttatatgaatttatttttt gcaggggggcattgtttggtaggtgagagatctgaattgctatgtttagtgagttgtatctatttatttttcaa- taaatacaattggttatgtgtttt gggggcgatcgtgaggcaaagaaaacccggcgctgaggccgggttattcttgttctctggtcaaattatatagt- tggaaaacaaggatgc atatatgaatgaacgatgcagaggcaatgccgatggcgatagtgggtatcatgtagccgcttatgctggaaaga- agcaataacccgcag aaaaacaaagctccaagctcaacaaaactaagggcatagacaataactaccgatgtcatatacccatactctct- aatcttggccagtcgg cgcgttctgcttccgattagaaacgtcaaggcagcaatcaggattgcaatcatggttcctgcatatgatgacaa- tgtcgccccaagaccat ctctatgagctgaaaaagaaacaccaggaatgtagtggcggaaaaggagatagcaaatgcttacgataacgtaa- ggaattattactatgt aaacaccaggcatgattctgttccgcataattactcctgataattaatccttaactttgcccacctgcctttta- aaacattccagtatatcactttt cattcttgcgtagcaatatgccatctcttcagctatctcagcattggtgaccttgttcagaggcgctgagagat- ggcctttttctgatagataat gttctgttaaaatatctccggcctcatcttttgcccgcaggctaatgtctgaaaattgaggtgacgggttaaaa- ataatatccttggcaaccttt tttatatcccttttaaattttggcttaatgactatatccaatgagtcaaaaagctccccttcaatatctgttgc- ccctaagacctttaatatatcgcc aaatacaggtagcttggcttctaccttcaccgttgttcggccgatgaaatgcatatgcataacatcgtctttgg- tggttcccctcatcagtggc tctatctgaacgcgctctccactgcttaatgacattcctttcccgattaaaaaatctgtcagatcggatgtggt- cggcccgaaaacagttctg gcaaaaccaatggtgtcgccttcaacaaacaaaaaagatgggaatcccaatgattcgtcatctgcgaggctgtt- cttaatatcttcaactga agctttagagcgatttatcttctgaaccagactcttgtcatttgttttggtaaagagaaaagtttttccatcga- ttttatgaatatacaaataattg gagccaacctgcaggtgatgattatcagccagcagagaattaaggaaaacagacaggtttattgagcgcttatc- tttccctttatttttgctg cggtaagtcgcataaaaaccattcttcataattcaatccatttactatgttatgttctgaggggagtgaaaatt- cccctaattcgatgaagattc ttgctcaattgttatcagctatgcgccgaccagaacaccttgccgatcagccaaacgtctcttcaggccactga- ctagcgataactttcccc acaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgct- atccctgatcagtttcttg aaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgag- aattaacattccgtca ggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcact- ggcttttttggttgtgctt acccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaa- aacagggtactcatact cacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaat- tcttcaacgctaactttgag aatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgact- gccccatccccatcttgtct gcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctc- aagctgctcttgtgttaatggtt tcttttttgtgctcatacgttaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgactatttt- acctctggcggtgataatggtt gcatgtactaaggaggttgtatggaacaacgcataaccctgaaagattatgcaatgcgctttgggcaaaccaag- acagctaaagatctcg gcgtatatcaaagcgcgatcaacaaggccattcatgcaggccgaaagatttttttaactataaacgctgatgga- agcgtttatgcggaaga ggtaaagcccttcccgagtaacaaaaaaacaacagcataaataaccccgctcttacacattccagccctgaaaa- agggcatcaaattaa accacacctatggtgtatgcatttatttgcatacattcaatcaattgttatctaaggaaatacttacatatggt- tcgtgcaaacaaacgcaacg aggctctacgaatcgagagtgcgttgcttaacaaaatcgcaatgcttggaactgagaagacagcggaagctgtg- ggcgttgataagtcg cagatcagcaggtggaagagggactggattccaaagttctcaatgctgcttgctgttcttgaatggggggtcgt- tgacgacgacatggct cgattggcgcgacaagttgctgcgattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaat- ccagatggagttctg

aggtcattactggatctatcaacaggagtcattatgacaaatacagcaaaaatactcaacttcggcagaggtaa- ctttgccggacaggag cgtaatgtggcagatctcgatgatggttacgccagactatcaaatatgctgcttgaggcttattcgggcgcaga- tctgaccaagcgacagt ttaaagtgctgcttgccattctgcgtaaaacctatgggtggaataaaccaatggacagaatcaccgattctcaa- cttagcgagattacaaag ttacctgtcaaacggtgcaatgaagccaagttagaactcgtcagaatgaatattatcaagcagcaaggcggcat- gtttggaccaaataaa aacatctcagaatggtgcatccctcaaaacgagggaaaatcccctaaaacgagggataaaacatccctcaaatt- gggggattgctatcc ctcaaaacagggggacacaaaagacactattacaaaagaaaaaagaaaagattattcgtcagannnnnntggcg- aatcctctgacca gccagaaaacgacctttctgtggtgaaaccggatgctgcaattcagagcggcagcaagtgggggacagcagaag- acctgaccgccg cagagtggatgtttgacatggtgaagactatcgcaccatcagccagaaaaccgaattttgctgggtgggctaac- gatatccgcctgatgc gtgaacgtgacggacgtaaccaccgcgacatgtgtgtgctgttccgctgggcatgccaggacaacttctggtcc- ggtaacgtgctgagc ccggccaaactccgcgataagtggacccaactcgaaatcaaccgtaacaagcaacaggcaggcgtgacagccag- caaaccaaaact cgacctgacaaacacagactggatttacggggtggatctatgaaaaacatcgccgcacagatggttaactttga- ccgtgagcagatgcg tcggatcgccaacaacatgccggaacagtacgacgaaaagccgcaggtacagcaggtagcgcagatcatcaacg- gtgtgttcagcca gttactggcaactttcccggcgagcctggctaaccgtgaccagaacgaagtgaacgaaatccgtcgccagtggg- ttctggcttttcggg aaaacgggatcaccacgatggaacaggttaacgcaggaatgcgcgtagcccgtcggcagaatcgaccatttctg- ccatcacccgggc agtttgttgcatggtgccgggaagaagcatccgttaccgccggactgccaaacgtcagcgagctggttgatatg- gtttacgagtattgcc ggaagcgaggcctgtatccggatgcggagtcttatccgtggaaatcaaacgcgcactactggctggttaccaac- ctgtatcagaacatg cgggccaatgcgcttactgatgcggaattacgccgtaaggccgcagatgagcttgtccatatgactgcgagaat- taaccgtggtgaggc gatccctgaaccagtaaaacaacttcctgtcatgggcggtagacctctaaatcgtgcacaggctctggcgaaga- tcgcagaaatcaaag ctaagttcggactgaaaggagcaagtgtatgacgggcaaagaggcaattattcattacctggggacgcataata- gcttctgtgcgccgg acgttgccgcgctaacaggcgcaacagtaaccagcataaatcaggccgcggctaaaatggcacgggcaggtctt- ctggttatcgaagg taaggtctggcgaacggtgtattaccggtttgctaccagggaagaacgggaaggaaagatgagcgatgaacaaa- ctggatacgattgg attcgacaacaaaaaagacctgcttatctcggtgggcgatttggttgatcgtggtgcagagaacgttgaatgcc- tggaattaatcacattcc cctggttcagagctgtacgtggaaaccatgagcaaatgatgattgatggcttatcagagcgtggaaacgttaat- cactggctgcttaatgg cggtggctggttctttaatctcgattacgacaaagaaattctggctaaagctcttgcccataaagcagatgaac- ttccgttaatcatcgaact ggtgagcaaagataaaaaatatgttatctgccacgccgattatccctttgacgaatacgagtttggaaagccag- ttgatcatcagcaggta atctggaaccgcgaacgaatcagcaactcacaaaacgggatcgtgaaagaaatcaaaggcgcggacacgttcat- ctttggtcatacgc cagcagtgaaaccactcaagtttgccaaccaaatgtatatcgataccggcgcagtgttctgcggaaacctaaca- ttgattcaggtacagg gagaaggcgcatgagactcgaaagcgtagctaaatttcattcgccaaaaagcccgatgatgagcgactcaccac- gggccacggcttct gactctctttccggtactgatgtgatggctgctatggggatggcgcaatcacaagccggattcggtatggctgc- attctgcggtaagcacg aactcagccagaacgacaaacaaaaggctatcaactatctgatgcaatttgcacacaaggtatcggggaaatac- cgtggtgtggcaaag cttgaaggaaatactaaggcaaaggtactgcaagtgctcgcaacattcgcttatgcggattattgccgtagtgc- cgcgacgccgggggc aagatgcagagattgccatggtacaggccgtgcggttgatattgccaaaacagagctgtgggggagagttgtcg- agaaagagtgcgga agatgcaaaggcgtcggctattcaaggatgccagcaagcgcagcatatcgcgctgtgacgatgctaatcccaaa- ccttacccaaccca cctggtcacgcactgttaagccgctgtatgacgctctggtggtgcaatgccacaaagaagagtcaatcgcagac- aacattttgaatgcgg tcacacgttagcagcatgattgccacggatggcaacatattaacggcatgatattgacttattgaataaaattg- ggtaaatttgactcaacga tgggttaattcgctcgttgtggtagtgagatgaaaagaggcggcgcttactaccgattccgcctagttggtcac- ttcgacgtatcgtctgga actccaaccatcgcaggcagagaggtctgcaaaatgcaatcccgaaacagttcgcaggtaatagttagagcctg- cataacggtttcggg attttttatatctgcacaacaggtaagagcattgagtcgataatcgtgaagagtcggcgagcctggttagccag- tgctctttccgttgtgctg aattaagcgaataccggaagcagaaccggatcaccaaatgcgtacaggcgtcatcgccgcccagcaacagcaca- acccaaactgag ccgtagccactgtctgtcctnnnnnnattagtaatagttacgctgcggccttttacacatgaccttcgtgaaag- cgggtggcaggaggtc gcgctaacaacctcctgccgttttgcccgtgcatatcggtcacgaacaaatctgattactaaacacagtagcct- ggatttgttctatcagtaa tcgaccttattcctaattaaatagagcaaatcccatattgggggtaagacatgaagatgccagaaaaacatgac- ctgttggccgccattct cgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcg- gtgcgtttacaaaaa cagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagt- agcaatctcgcttatataa cgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagcc- ggagtagaagatggtag aaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaa- accagaaatcatggt tatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcacgctaaaccc- aaaactcaaatcaacagg cgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctc- cgaaaagtcaggacg ctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatc- gaccgttgcagcaatat ctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaag- aagcgggcggaacg gtcagagagattgatgtatgagcagagtcaccgcgattatctccgctctggttatctgcatcatcgtctgcctg- tcatgggctgttaatcatta ccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgaagctggcgaacgcggcaa- ttactgacatgca gatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgctaaagctgaaaatg- atgctctgcgtgatgat gttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccaccgcctccgg- cgtggataatgcagc ctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaac- aactggaaggaacc cagaagtatattaatgagcagtgcagatagagttgcccatatcgatgggcaactcatgcaattattgtgagcaa- tacacacgcgcttccag cggagtataaatgcctaaagtaataaaaccgagcaatccatttacgaatgtttgctgggtttctgttttaacaa- cattttctgcgccgccacaa attttggctgcatcgacagttttcttctgcccaattccagaaacgaagaaatgatgggtgatggtttcctttgg- tgctactgctgccggtttgtt ttgaacagtaaacgtctgttgagcacatcctgtaataagcagggccagcgcagtagcgagtagcatttttttca- tggtgttattcccgatgct ttttgaagttcgcagaatcgtatgtgtagaaaattaaacaaaccctaaacaatgagttgaaatttcatattgtt- aatatttattaatgtatgtcag gtgcgatgaatcgtcattgtattcccggattaactatgtccacagccctgacggggaacttctctgcgggagtg- tccgggaataattaaaa cgatgcacacagggtttagcgcgtacacgtattgcattatgccaacgccccggtgctgacacggaagaaaccgg- acgttatgatttagc gtggaaagatttgtgtagtgttctgaatgctctcagtaaatagtaatgaattatcaaaggtatagtaatatctt- ttatgttcatggatatttgtaac ccatcggaaaactcctgctttagcaagattttccctgtattgctgaaatgtgatttctcttgatttcaacctat- cataggacgtttctataagatg cgtgtttcttgagaatttaacatttacaacctttttaagtccttttattaacacggtgttatcgttttctaaca- cgatgtgaatattatctgtggctag atagtaaatataatgtgagacgttgtgacgttttagttcagaataaaacaattcacagtctaaatcttttcgca- cttgatcgaatatttctttaaaa atggcaacctgagccattggtaaaaccttccatgtgatacgagggcgcgtagtttgcattatcgtttttatcgt- ttcaatctggtctgacctcct tgtgttttgttgatgatttatgtcaaatattaggaatgttttcacttaatagtattggttgcgtaacaaagtgc- ggtcctgctggcattctggagg gaaatacaaccgacagatgtatgtaaggccaacgtgctcaaatcttcatacagaaagatttgaagtaatatttt- aaccgctagatgaagag caagcgcatggagcgacaaaatgaataaagaacaatctgctgatgatccctccgtggatctgattcgtgtaaaa- aatatgcttaatagcac catttctatgagttaccctgatgttgtaattgcatgtatagaacataaggtgtctctggaagcattcagagcaa- ttgaggcagcgttggtgaa gcacgataataatatgaaggattattccctggtggttgactgatcaccataactgctaatcattcaaactattt- agtctgtgacagagccaac acgcagtctgtcactgtcaggaaagtggtaaaactgcaactcaattactgcaatgccctcgtaattaagtgaat- ttacaatatcgtcctgttc ggagggaagaacgcgggatgttcattcttcatcacttttaattgatgtatatgctctcttttctgacgttagtc- tccgacggcaggcttcaatg acccaggctgagaaattcccggaccctttttgctcaagagcgatgttaatttgttcaatcatttggttaggaaa- gcggatgttgcgggttgtt gttctgcgggttctgttcttcgttgacatgaggttgccccgtattcagtgtcgctgatttgtattgtctgaagt- tgtttttacgttaagttgatgca gatcaattaatacgatacctgcgtcataattgattatttgacgtggtttgatggcctccacgcacgttgtgata- tgtagatgataatcattatca ctttacgggtcctttccggtgatccgacaggttacg

TABLE-US-00010 TABLE 10 Sequence of .lamda.::Tn-I-Sce2-PsapLuxAB-Cm5 (SEQ ID NO: 4). ctgtctcttatacacatctcaaccctgaagctcttgttggctagtgcgtagtcgttggcaagcttgcatgcctg- cagtagggataacagggt aatgtcgacttgcatgcctgcataaatttgaattttataaaaaattatgttataattcgcgcagtacgatggat- aatgatcatctggatattgatg ttgatattactgaaccgtcatttgaacatttaactattgcgacagtcaatgaacgccgaatgagaattgagatt- gaaaatcaagcaatttctct gtcttaaaatctattgagatattctatcactcaaatagcaatataaggactctctatgaaatttggaaactttt- tgcttacataccaacctcccca attttctcaaacagaggtaatgaaacgtttggttaaattaggtcgcatctctgaggagtgtggttttgataccg- tatggttactggagcatcatt tcacggagtttggtttgcttggtaacccttatgtcgctgctgcatatttacttggcgcgactaaaaaattgaat- gtaggaactgccgctattgtt cttcccacagcccatccagtacgccaacttgaagatgtgaatttattggatcaaatgtcaaaaggacgatttcg- gtttggtatttgccgagg gctttacaacaaggactttcgcgtattcggcacagatatgaataacagtcgcgccttagcggaatgctggtacg- ggctgataaagaatgg catgacagagggatatatggaagctgataatgaacatatcaagttccataaggtaaaagtaaaccccgcggcgt- atagcagaggtggcg caccggtttatgtggtggctgaatcagcttcgacgactgagtgggctgctcaatttggcctaccgatgatatta- agttggattataaatacta acgaaaagaaagcacaacttgagctttataatgaagtggctcaagaatatgggcacgatattcataatatcgac- cattgcttatcatatataa catctgtagatcatgactcaattaaagcgaaagagatttgccggaaatttctggggcattggtatgattcttat- gtgaatgctacgactattttt gatgattcagaccaaacaagaggttatgatttcaataaagggcagtggcgtgactttgtattaaaaggacataa- agatactaatcgccgtat tgattacagttacgaaatcaatcccgtgggaacgccgcaggaatgtattgacataattcaaaaagacattgatg- ctacaggaatatcaaat atttgttgtggatttgaagctaatggaacagtagacgaaattattgcttccatgaagctcttccagtctgatgt- catgccatttcttaaagaaaa acaacgttcgctattatattagctaaggagaaagaaatgaaatttggattgttcttccttaacttcatcaattc- aacaactgttcaagaacaaa gtatagttcgcatgcaggaaataacggagtatgttgataagttgaattttgaacagattttagtgtatgaaaat- catttttcagataatggtgttg tcggcgctcctctgactgtttctggttttctgctcggtttaacagagaaaattaaaattggttcattaaatcac- atcattacaactcatcatcctgt cgccatagcggaggaagcttgcttattggatcagttaagtgaagggagatttattttagggtttagtgattgcg- aaaaaaaagatgaaatgc atttttttaatcgcccggttgaatatcaacagcaactatttgaagagtgttatgaaatcattaacgatgcttta- acaacaggctattgtaatcca gataacgatttttatagcttccctaaaatatctgtaaatccccatgcttatacgccaggcggacctcggaaata- tgtaacagcaaccagtcat catattgttgagtgggcggccaaaaaaggtattcctctcatctttaagtgggatgattctaatgatgttagata- tgaatatgctgaaagatata aagccgttgcggataaatatgacgttgacctatcagagatagaccatcagttaatgatattagttaactataac- gaagatagtaataaagct aaacaagagacgcgtgcatttattagtgattatgttcttgaaatgcaccctaatgaaaatttcgaaaataaact- tgaagaaataattgcagaa aacgctgtcggaaattatacggagtgtataactgcggctaagttggcaattgaaaagtgtggtgcgaaaagtgt- attgctgtcctttgaacc aatgaatgatttgatgagccaaaaaaatgtaatcaatattgttgatgataatattaagaagtaccacatggaat- atacctaatagatttcgagtt gcagcgaggcggcaagtgaacgaatccccaggagcatagataactatgtgactggggtgagtgaaagcagccaa- caaagcagcagc ttgaaagatgaagggtataaaagagtatgacagcagtgctgccatactttctaatattatcttgaggagtaaaa- caggtatgacttcatatgtt gataaacaagaaattacagcaagctcagaaattgatgatttgattttttcgagcgatccatagatctggagcta- aggaagctaaaatggag aaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtc- agttgctcaatgtacctata accagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcc- tttattcacattcttgccc gcctgatgaatgctcatccggaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcac- ccttgttacaccgttttc catgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatata- ttcgcaagatgtggcgtg ttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctggg- tgagtttcaccagttttgattt aaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaagg- tgctgatgccgctggc gattcaggttcatcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcg- atgagtggcagggcggg gcgtaatttcaattcattaccctgttatccctacccgagaaaattcatcgatgatggttgagatgtgtataaga- gacaggttaaatctatcacc gcaagggataaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaa- ggaggttgtatggaacaa cgcataaccctgaaagattatgcaatgcgctttgggcaaaccaagacagctaaagatctcggcgtatatcaaag- cgcgatcaacaaggc cattcatgcaggccgaaagatttttttaactataaacgctgatggaagcgtttatgcggaagaggtaaagccct- tcccgagtaacaaaaaa acaacagcataaataaccccgctcttacacattccagccctgaaaaagggcatcaaattaaaccacacctatgg- tgtatgcatttatttgca tacattcaatcaattgttatctaaggaaatacttacatatggttcgtgcaaacaaacgcaacgaggctctacga- atcgagagtgcgttgctta acaaaatcgcaatgcttggaactgagaagacagcggaagctgtgggcgttgataagtcgcagatcagcaggtgg- aagagggactgga ttccaaagttctcaatgctgcttgctgttcttgaatggggggtcgttgacgacgacatggctcgattggcgcga- caagttgctgcgattctc accaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggat- ctatcaacaggagtc attatgacaaatacagcaaaaatactcaacttcggcagaggtaactttgccggacaggagcgtaatgtggcaga- tctcgatgatggttac gccagactatcaaatatgctgcttgaggcttattcgggcgcagatctgaccaagcgacagtttaaagtgctgct- tgccattctgcgtaaaac ctatgggtggaataaaccaatggacagaatcaccgattctcaacttagcgagattacaaagttacctgtcaaac- ggtgcaatgaagccaa gttagaactcgtcagaatgaatattatcaagcagcaaggcggcatgtttggaccaaataaaaacatctcagaat- ggtgcatccctcaaaac gagggaaaatcccctaaaacgagggataaaacatccctcaaattgggggattgctatccctcaaaacaggggga- cacaaaagacacta ttacaaaagaaaaaagaaaagattattcgtcagagaattctggcgaatcctctgaccagccagaaaacgacctt- tctgtggtgaaaccgg atgctgcaattcagagcggcagcaagtgggggacagcagaagacctgaccgccgcagagtggatgtttgacatg- gtgaagactatcg caccatcagccagaaaaccgaattttgctgggtgggctaacgatatccgcctgatgcgtgaacgtgacggacgt- aaccaccgcgacat gtgtgtgctgttccgctgggcatgccaggacaacttctggtccggtaacgtgctgagcccggccaaactccgcg- ataagtggacccaac tcgaaatcaaccgtaacaagcaacaggcaggcgtgacagccagcaaaccaaaactcgacctgacaaacacagac- tggatttacgggg tggatctatgaaaaacatcgccgcacagatggttaactttgaccgtgagcagatgcgtcggatcgccaacaaca- tgccggaacagtacg acgaaaagccgcaggtacagcaggtagcgcagatcatcaacggtgtgttcagccagttactggcaactttcccg- gcgagcctggctaa ccgtgaccagaacgaagtgaacgaaatccgtcgccagtgggttctggcttttcgggaaaacgggatcaccacga- tggaacaggttaac gcaggaatgcgcgtagcccgtcggcagaatcgaccatttctgccatcacccgggcagtttgttgcatggtgccg- ggaagaagcatccgt taccgccggactgccaaacgtcagcgagctggttgatatggtttacgagtattgccggaagcgaggcctgtatc- cggatgcggagtctta tccgtggaaatcaaacgcgcactactggctggttaccaacctgtatcagaacatgcgggccaatgcgcttactg- atgcggaattacgccg taaggccgcagatgagcttgtccatatgactgcgagaattaaccgtggtgaggcgatccctgaaccagtaaaac- aacttcctgtcatggg cggtagacctctaaatcgtgcacaggctctggcgaagatcgcagaaatcaaagctaagttcggactgaaaggag- caagtgtatgacgg gcaaagaggcaattattcattacctggggacgcataatagcttctgtgcgccggacgttgccgcgctaacaggc- gcaacagtaaccagc ataaatcaggccgcggctaaaatggcacgggcaggtcttctggttatcgaaggtaaggtctggcgaacggtgta- ttaccggtttgctacc agggaagaacgggaaggaaagatgagcacgaacctggtttttaaggagtgtcgccagagtgccgcgatgaaacg- ggtattggcggta tatggagttaaaagatgaccatctacattactgagctaataacaggcctgctggtaatcgcaggcctttttatt- tgggggagagggaagtca tgaaaaaactaacctttgaaattcgatctccagcacatcagcaaaacgctattcacgcagtacagcaaatcctt- ccagacccaaccaaac caatcgtagtaaccattcaggaacgcaaccgcagcttagaccaaaacaggaagctatgggcctgcttaggtgac- gtctctcgtcaggttg aatggcatggtcgctggctggatgcagaaagctggaagtgtgtgtttaccgcagcattaaagcagcaggatgtt- gttcctaaccttgccg ggaatggctttgtggtaataggccagtcaaccagcaggatgcgtgtaggcgaatttgcggagctattagagctt- atacaggcattcggta cagagcgtggcgttaagtggtcagacgaagcgagactggctctggagtggaaagcgagatggggagacagggct- gcatgataaatgt cgttagtttctccggtggcaggacgtcagcatatttgctctggctaatggagcaaaagcgacgggcaggtaaag- acgtgcattacgttttc atggatacaggttgtgaacatccaatgacatatcggtttgtcagggaagttgtgaagttctgggatataccgct- caccgtattgcaggttgat atcaacccggagcttggacagccaaatggttatacggtatgggaaccaaaggatattcagacgcgaatgcctgt- tctgaagccatttatc gatatggtaaagaaatatggcactccatacgtcggcggcgcgttctgcactgacagattaaaactcgttccctt- caccaaatactgtgatg accatttcgggcgagggaattacaccacgtggattggcatcagagctgatgaaccgaagcggctaaagccaaag- cctggaatcagata tcttgctgaactgtcagactttgagaaggaagatatcctcgcatggtggaagcaacaaccattcgatttgcaaa- taccggaacatctcggt aactgcatattctgcattaaaaaatcaacgcaaaaaatcggacttgcctgcaaagatgaggagggattgcagcg- tgtttttaatgaggtcat cacgggatcccatgtgcgtgacggacatcgggaaacgccaaaggagattatgtaccgaggaagaatgtcgctgg- acggtatcgcgaa aatgtattcagaaaatgattatcaagccctgtatcaggacatggtacgagctaaaagattcgataccggctctt- gttctgagtcatgcgaaat atttggagggcagcttgatttcgacttcgggagggaagctgcatgatgcgatgttatcggtgcggtgaatgcaa- agaagataaccgcttc

cgaccaaatcaaccttactggaatcgatggtgtctccggtgtgaaagaacaccaacaggggtgttaccactacc- gcaggaaaaggagg acgtgtggcgagacagcgacgaagtatcaccgacataatctgcgaaaactgcaaataccttccaacgaaacgca- ccagaaataaaccc aagccaatcccaaaagaatctgacgtaaaaaccttcaactacacggctcacctgtgggatatccggtggctaag- acgtcgtgcgaggaa aacaaggtgattgaccaaaatcgaagttacgaacaagaaagcgtcgagcgagctttaacgtgcgctaactgcgg- tcagaagctgcatgt gctggaagttcacgtgtgtgagcactgctgcgcagaactgatgagcgatccgaatagctcgatgcacgaggaag- aagatgatggctaa accagcgcgaagacgatgtaaaaacgatgaatgccgggaatggtttcaccctgcattcgctaatcagtggtggt- gctctccagagtgtg gaaccaagatagcactcgaacgacgaagtaaagaacgcgaaaaagcggaaaaagcagcagagaagaaacgacga- cgagaggag cagaaacagaaagataaacttaagattcgaaaactcgccttaaagccccgcagttactggattaaacaagccca- acaagccgtaaacgc cttcatcagagaaagagaccgcgacttaccatgtatctcgtgcggaacgctcacgtctgctcagtgggatgccg- gacattaccggacaa ctgctgcggcacctcaactccgatttaatgaacgcaatattcacaagcaatgcgtggtgtgcaaccagcacaaa- agcggaaatctcgttc cgtatcgcgtcgaactgattagccgcatcgggcaggaagcagtagacgaaatcgaatcaaaccataaccgccat- cgctggactatcga agagtgcaaggcgatcaaggcagagtaccaacagaaactcaaagacctgcgaaatagcagaagtgaggccgcat- gacgttctcagta aaaaccattccagacatgctcgttgaagcatacggaaatcagacagaagtagcacgcagactgaaatgtagtcg- cggtacggtcagaa aatacgttgatgataaagacgggaaaatgcacgccatcgtcaacgacgttctcatggttcatcgcggatggagt- gaaagagatgcgctat tacgaaaaaattgatggcagcaaataccgaaatatttgggtagttggcgatctgcacggatgctacacgaacct- gatgaacaaactggat acgattggattcgacaacaaaaaagacctgcttatctcggtgggcgatttggttgatcgtggtgcagagaacgt- tgaatgcctggaattaa tcacattcccctggttcagagctgtacgtggaaaccatgagcaaatgatgattgatggcttatcagagcgtgga- aacgttaatcactggct gcttaatggcggtggctggttctttaatctcgattacgacaaagaaattctggctaaagctcttgcccataaag- cagatgaacttccgttaat catcgaactggtgagcaaagataaaaaatatgttatctgccacgccgattatccctttgacgaatacgagtttg- gaaagccagttgatcatc agcaggtaatctggaaccgcgaacgaatcagcaactcacaaaacgggatcgtgaaagaaatcaaaggcgcggac- acgttcatctttgg tcatacgccagcagtgaaaccactcaagtttgccaaccaaatgtatatcgataccggcgcagtgttctgcggaa- acctaacattgattcag gtacagggagaaggcgcatgagactcgaaagcgtagctaaatttcattcgccaaaaagcccgatgatgagcgac- tcaccacgggcca cggcttctgactctctttccggtactgatgtgatggctgctatggggatggcgcaatcacaagccggattcggt- atggctgcattctgcggt aagcacgaactcagccagaacgacaaacaaaaggctatcaactatctgatgcaatttgcacacaaggtatcggg- gaaataccgtggtgt ggcaaagcttgaaggaaatactaaggcaaaggtactgcaagtgctcgcaacattcgcttatgcggattattgcc- gtagtgccgcgacgc cgggggcaagatgcagagattgccatggtacaggccgtgcggttgatattgccaaaacagagctgtgggggaga- gttgtcgagaaag agtgcggaagatgcaaaggcgtcggctattcaaggatgccagcaagcgcagcatatcgcgctgtgacgatgcta- atcccaaaccttac ccaacccacctggtcacgcactgttaagccgctgtatgacgctctggtggtgcaatgccacaaagaagagtcaa- tcgcagacaacatttt gaatgcggtcacacgttagcagcatgattgccacggatggcaacatattaacggcatgatattgacttattgaa- taaaattgggtaaatttg actcaacgatgggttaattcgctcgttgtggtagtgagatgaaaagaggcggcgcttactaccgattccgccta- gttggtcacttcgacgt atcgtctggaactccaaccatcgcaggcagagaggtctgcaaaatgcaatcccgaaacagttcgcaggtaatag- ttagagcctgcataa cggtttcgggattttttatatctgcacaacaggtaagagcattgagtcgataatcgtgaagagtcggcgagcct- ggttagccagtgctctttc cgttgtgctgaattaagcgaataccggaagcagaaccggatcaccaaatgcgtacaggcgtcatcgccgcccag- caacagcacaacc caaactgagccgtagccactgtctgtcctgaattcattagtaatagttacgctgcggccttttacacatgacct- tcgtgaaagcgggtggca ggaggtcgcgctaacaacctcctgccgttttgcccgtgcatatcggtcacgaacaaatctgattactaaacaca- gtagcctggatttgttct atcagtaatcgaccttattcctaattaaatagagcaaatccccttattgggggtaagacatgaagatgccagaa- aaacatgacctgttggcc gccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagata- taatggcggtgcgttt acaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccgg- actaagtagcaatctcg cttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgct- aaaaaagccggagtagaa gatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggac- gtcagaaaaccaga aatcatggttatgacgtcattgtaggcggagagctatttactgattactccgatcaccctcgcaaacttgtcac- gctaaacccaaaactcaa atcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaag- acttctctccgaaaag tcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtc- aggcaatcgaccgttgc agcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaa- attcaaagaagcggg cggaacggtcagagagattgatgtatgagcagagtcaccgcgattatctccgctctggttatctgcatcatcgt- ctgcctgtcatgggctgt taatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgaagctggcga- acgcggcaattactg acatgcagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgctaaagct- gaaaatgatgctctgc gtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccaccacc- gcctccggcgtggata atgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatg- caaaaacaactgga aggaacccagaagtatattaatgagcagtgcagatagagttgcccatatcgatgggcaactcatgcaattattg- tgagcaatacacacgc gcttccagcggagtataaatgcctaaagtaataaaaccgagcaatccatttacgaatgtttgctgggtttctgt- tttaacaacattttctgcgc cgccacaaattttggctgcatcgacagttttcttctgcccaattccagaaacgaagaaatgatgggtgatggtt- tcctttggtgctactgctg ccggtttgttttgaacagtaaacgtctgttgagcacatcctgtaataagcagggccagcgcagtagcgagtagc- atttttttcatggtgttatt cccgatgctttttgaagttcgcagaatcgtatgtgtagaaaattaaacaaaccctaaacaatgagttgaaattt- catattgttaatatttattaat gtatgtcaggtgcgatgaatcgtcattgtattcccggattaactatgtccacagccctgacggggaacttctct- gcgggagtgtccgggaa taattaaaacgatgcacacagggtttagcgcgtacacgtattgcattatgccaacgccccggtgctgacacgga- agaaaccggacgttat gatttagcgtggaaagatttgtgtagtgttctgaatgctctcagtaaatagtaatgaattatcaaaggtatagt- aatatcttttatgttcatggata tttgtaacccatcggaaaactcctgctttagcaagattttccctgtattgctgaaatgtgatttctcttgattt- caacctatcataggacgtttctat aagatgcgtgtttcttgagaatttaacatttacaacctttttaagtccttttattaacacggtgttatcgtttt- ctaacacgatgtgaatattatctgt ggctagatagtaaatataatgtgagacgttgtgacgttttagttcagaataaaacaattcacagtctaaatctt- ttcgcacttgatcgaatatttc tttaaaaatggcaacctgagccattggtaaaaccttccatgtgatacgagggcgcgtagtttgcattatcgttt- ttatcgtttcaatctggtctg acctccttgtgttttgttgatgatttatgtcaaatattaggaatgttttcacttaatagtattggttgcgtaac- aaagtgcggtcctgctggcattct ggagggaaatacaaccgacagatgtatgtaaggccaacgtgctcaaatcttcatacagaaagatttgaagtaat- attttaaccgctagatg aagagcaagcgcatggagcgacaaaatgaataaagaacaatctgctgatgatccctccgtggatctgattcgtg- taaaaaatatgcttaat agcaccatttctatgagttaccctgatgttgtaattgcatgtatagaacataaggtgtctctggaagcattcag- agcaattgaggcagcgttg gtgaagcacgataataatatgaaggattattccctggtggttgactgatcaccataactgctaatcattcaaac- tatttagtctgtgacagag ccaacacgcagtctgtcactgtcaggaaagtggtaaaactgcaactcaattactgcaatgccctcgtaattaag- tgaatttacaatatcgtc ctgttcggagggaagaacgcgggatgttcattcttcatcacttttaattgatgtatatgctctcttttctgacg- ttagtctccgacggcaggctt caatgacccaggctgagaaattcccggaccctttttgctcaagagcgatgttaatttgttcaatcatttggtta- ggaaagcggatgttgcgg gttgttgttctgcgggttctgttcttcgttgacatgaggttgccccgtattcagtgtcgctgatttgtattgtc- tgaagttgtttttacgttaagttg atgcagatcaattaatacgatacctgcgtcataattgattatttgacgtggtttgatggcctccacgcacgttg- tgatatgtagatgataatcat tatcactttacgggtcctttccggtgatccgacaggttacggggcggcgacctcgcgggttttcgctatttatg- aaaattttccggtttaagg cgtttccgttcttcttcgtcataacttaatgtttttatttaaaataccctctgaaaagaaaggaaacgacaggt- gctgaaagcgaggctttttgg cctctgtcgtttcctttctctgtttttgtccgtggaatgaacaatggaagtcaacaaaaagcagctggctgaca- ttttcggtgcgagtatccgt accattcagaactggcaggaacagggaatgcccgttctgcgaggcggtggcaagggtaatgaggtgctttatga- ctctgccgccgtcat aaaatggtatgccgaaagggatgctgaaattgagaacgaaaagctgcgccgggaggttgaagaactgcggcagg- ccagcgaggca gatctccagccaggaactattgagtacgaacgccatcgacttacgcgtgcgcaggccgacgcacaggaactgaa- gaatgccagagac tccgctgaagtggtggaaaccgcattctgtactttcgtgctgtcgcggatcgcaggtgaaattgccagtattct- cgacgggctccccctgt cggtgcagcggcgttttccggaactggaaaaccgacatgttgatttcctgaaacgggatatcatcaaagccatg- aacaaagcagccgcg ctggatgaactgataccggggttgctgagtgaatatatcgaacagtcaggttaacaggctgcggcattttgtcc- gcgccgggcttcgctc actgttcaggccggagccacagaccgccgttgaatgggcggatgctaattactatctcccgaaagaatccgcat- accaggaagggcgc tgggaaacactgccctttcagcgggccatcatgaatgcgatgggcagcgactacatccgtgaggtgaatgtggt- gaagtctgcccgtgt cggttattccaaaatgctgctgggtgtttatgcctactttatagagcataagcagcgcaacacccttatctggt- tgccgacggatggtgatg ccgagaactttatgaaaacccacgttgagccgactattcgtgatattccgtcgctgctggcgctggccccgtgg-

tatggcaaaaagcacc gggataacacgctcaccatgaagcgtttcactaatgggcgtggcttctggtgcctgggcggtaaagcggcaaaa- aactaccgtgaaaa gtcggtggatgtggcgggttatgatgaacttgctgcttttgatgatgatattgaacaggaaggctctccgacgt- tcctgggtgacaagcgta ttgaaggctcggtctggccaaagtccatccgtggctccacgccaaaagtgagaggcacctgtcagattgagcgt- gcagccagtgaatc cccgcattttatgcgttttcatgttgcctgcccgcattgcggggaggagcagtatcttaaatttggcgacaaag- agacgccgtttggcctca aatggacgccggatgacccctccagcgtgttttatctctgcgagcataatgcctgcgtcatccgccagcaggag- ctggactttactgatgc ccgttatatctgcgaaaagaccgggatctggacccgtgatggcattctctggttttcgtcatccggtgaagaga- ttgagccacctgacagt gtgacctttcacatctggacagcgtacagcccgttcaccacctgggtgcagattgtcaaagactggatgaaaac- gaaaggggatacgg gaaaacgtaaaaccttcgtaaacaccacgctcggtgagacgtgggaggcgaaaattggcgaacgtccggatgct- gaagtgatggcag agcggaaagagcattattcagcgcccgttcctgaccgtgtggcttacctgaccgccggtatcgactcccagctg- gaccgctacgaaatg cgcgtatggggatgggggccgggtgaggaaagctggctgattgaccggcagattattatgggccgccacgacga- tgaacagacgctg ctgcgtgtggatgaggccatcaataaaacctatacccgccggaatggtgcagaaatgtcgatatcccgtatctg- ctgggatactggcgg gattgacccgaccattgtgtatgaacgctcgaaaaaacatgggctgttccgggtgatccccattaaaggggcat- ccgtctacggaaagc cggtggccagcatgccacgtaagcgaaacaaaaacggggtttaccttaccgaaatcggtacggataccgcgaaa- gagcagatttataa ccgcttcacactgacgccggaaggggatgaaccgcttcccggtgccgttcacttcccgaataacccggatattt- ttgatctgaccgaagc gcagcagctgactgctgaagagcaggtcgaaaaatgggtggatggcaggaaaaaaatactgtgggacagcaaaa- agcgacgcaatg aggcactcgactgcttcgtttatgcgctggcggcgctgcgcatcagtatttcccgctggcagctggatctcagt- gcgctgctggcgagcc tgcaggaagaggatggtgcagcaaccaacaagaaaacactggcagattacgcccgtgccttatccggagaggat- gaatgacgcgaca ggaagaacttgccgctgcccgtgcggcactgcatgacctgatgacaggtaaacgggtggcaacagtacagaaag- acggacgaaggg tggagtttacggccacttccgtgtctgacctgaaaaaatatattgcagagctggaagtgcagaccggcatgaca- cagcgacgcagggg acctgcaggattttatgtatgaaaacgcccaccattcccacccttctggggccggacggcatgacatcgctgcg- cgaatatgccggttat cacggcggtggcagcggatttggagggcagttgcggtcgtggaacccaccgagtgaaagtgtggatgcagccct- gttgcccaacttta cccgtggcaatgcccgcgcagacgatctggtacgcaataacggctatgccgccaacgccatccagctgcatcag- gatcatatcgtcgg gtcttttttccggctcagtcatcgcccaagctggcgctatctgggcatcggggaggaagaagcccgtgcctttt- cccgcgaggttgaagc ggcatggaaagagtttgccgaggatgactgctgctgcattgacgttgagcgaaaacgcacgtttaccatgatga- ttcgggaaggtgtgg ccatgcacgcctttaacggtgaactgttcgttcaggccacctgggataccagttcgtcgcggcttttccggaca- cagttccggatggtcag cccgaagcgcatcagcaacccgaacaataccggcgacagccggaactgccgtgccggtgtgcagattaatgaca- gcggtgcggcgc tgggatattacgtcagcgaggacgggtatcctggctggatgccgcagaaatggacatggataccccgtgagtta- cccggcgggcgcg cctcgttcattcacgtttttgaacccgtggaggacgggcagactcgcggtgcaaatgtgttttacagcgtgatg- gagcagatgaagatgct cgacacgctgcagaacacgcagctgcagagcgccattgtgaaggcgatgtatgccgccaccattgagagtgagc- tggatacgcagtc agcgatggattttattctgggcgcgaacagtcaggagcagcgggaaaggctgaccggctggattggtgaaattg- ccgcgtattacgcc gcagcgccggtccggctgggaggcgcaaaagtaccgcacctgatgccgggtgactcactgaacctgcagacggc- tcaggatacgga taacggctactccgtgtttgagcagtcactgctgcggtatatcgctgccgggctgggtgtctcgtatgagcagc- tttcccggaattacgcc cagatgagctactccacggcacgggccagtgcgaacgagtcgtgggcgtactttatggggcggcgaaaattcgt- cgcatcccgtcagg cgagccagatgtttctgtgctggctggaagaggccatcgttcgccgcgtggtgacgttaccttcaaaagcgcgc- ttcagttttcaggaag cccgcagtgcctgggggaactgcgactggataggctccggtcgtatggccatcgatggtctgaaagaagttcag- gaagcggtgatgct gatagaagccggactgagtacctacgagaaagagtgcgcaaaacgcggtgacgactatcaggaaatttttgccc- agcaggtccgtgaa acgatggagcgccgtgcagccggtcttaaaccgcccgcctgggcggctgcagcatttgaatccgggctgcgaca- atcaacagaggag gagaagagtgacagcagagctgcgtaatctcccgcatattgccagcatggcctttaatgagccgctgatgcttg- aacccgcctatgcgc gggttttcttttgtgcgcttgcaggccagcttgggatcagcagcctgacggatgcggtgtccggcgacagcctg- actgcccaggaggca ctcgcgacgctggcattatccggtgatgatgacggaccacgacaggcccgcagttatcaggtcatgaacggcat- cgccgtgctgccgg tgtccggcacgctggtcagccggacgcgggcgctgcagccgtactcggggatgaccggttacaacggcattatc- gcccgtctgcaac aggctgccagcgatccgatggtggacggcattctgctcgatatggacacgcccggcgggatggtggcgggggca- tttgactgcgctg acatcatcgcccgtgtgcgtgacataaaaccggtatgggcgcttgccaacgacatgaactgcagtgcaggtcag- ttgcttgccagtgcc gcctcccggcgtctggtcacgcagaccgcccggacaggctccatcggcgtcatgatggctcacagtaattacgg- tgctgcgctggaga aacagggtgtggaaatcacgctgatttacagcggcagccataaggtggatggcaacccctacagccatcttccg- gatgacgtccggga gacactgcagtcccggatggacgcaacccgccagatgtttgcgcagaaggtgtcggcatataccggcctgtccg- tgcaggttgtgctg gataccgaggctgcagtgtacagcggtcaggaggccattgatgccggactggctgatgaacttgttaacagcac- cgatgcgatcaccgt catgcgtgatgcactggatgcacgtaaatcccgtctctcaggagggcgaatgaccaaagagactcaatcaacaa- ctgtttcagccactg cttcgcaggctgacgttactgacgtggtgccagcgacggagggcgagaacgccagcgcggcgcagccggacgtg- aacgcgcagat caccgcagcggttgcggcagaaaacagccgcattatggggatcctcaactgtgaggaggctcacggacgcgaag- aacaggcacgc gtgctggcagaaacccccggtatgaccgtgaaaacggcccgccgcattctggccgcagcaccacagagtgcaca- ggcgcgcagtga cactgcgctggatcgtctgatgcagggggcaccggcaccgctggctgcaggtaacccggcatctgatgccgtta- acgatttgctgaaca caccagtgtaagggatgtttatgacgagcaaagaaacctttacccattaccagccgcagggcaacagtgacccg- gctcataccgcaac cgcgcccggcggattgagtgcgaaagcgcctgcaatgaccccgctgatgctggacacctccagccgtaagctgg- ttgcgtgggatgg caccaccgacggtgctgccgttggcattcttgcggttgctgctgaccagaccagcaccacgctgacgttctaca- agtccggcacgttcc gttatgaggatgtgctctggccggaggctgccagcgacgagacgaaaaaacggaccgcgtttgccggaacggca- atcagcatcgttta actttacccttcatcactaaaggccgcctgtgcggattttttacgggatttttttatgtcgatgtacacaaccg- cccaactgctggcggcaaa tgagcagaaatttaagtttgatccgctgtttctgcgtctctttttccgtgagagctatcccttcaccacggaga- aagtctatctctcacaaattc cgggactggtaaacatggcgctgtacgtttcgccgattgtttccggtgaggttatccgttcccgtggcggctcc- acctctgaatttacgccg ggatatgtcaagccgaagcatgaagtgaatccgcagatgaccctgcgtcgcctgccggatgaagatccgcagaa- tctggcggacccg gcttaccgccgccgtcgcatcatcatgcagaacatgcgtgacgaagagctggccattgctcaggtcgaagagat- gcaggcagtttctgc cgtgcttaagggcaaatacaccatgaccggtgaagccttcgatccggttgaggtggatatgggccgcagtgagg- agaataacatcacg cagtccggcggcacggagtggagcaagcgtgacaagtccacgtatgacccgaccgacgatatcgaagcctacgc- gctgaacgccag cggtgtggtgaatatcatcgtgttcgatccgaaaggctgggcgctgttccgttccttcaaagccgtcaaggaga- agctggatacccgtcg tggactaattccgagaggagacagcggtgaaagacctgggcaaagcggtgtcctataaggggatgtatggcgat- gtggccatcgtc gtgtattccggacagtacgtggaaaacggcgtcaaaaagaacttcctgccggacaacacgatggtgctggggaa- cactcaggcacgc ggtctgcgcacctatggctgcattcaggatgcggacgcacagcgcgaaggcattaacgcctctgcccgttaccc- gaaaaactgggtga ccaccggcgatccggcgcgtgagttcaccatgattcagtcagcaccgctgatgctgctggctgaccctgatgag- ttcgtgtccgtacaac tggcgtaatcatggcccttcggggccattgtttctctgtggaggagtccatgacgaaagatgaactgattgccc- gtaccgctcgctgggt gaacaactgaaccgtgatgtcagcctgacggggacgaaagaagaactggcgctccgtgtggcagagagaaagag- gagcttgatga cacggatgaaactgccggtcaggacacccctctcagccgggaaaatgtgctgaccggacatgaaaatgaggtgg- gatcagcgcagc cggataccgtgattctggatacgtctgaactggtcacggtcgtggcactggtgaagctgcatactgatgcactt- cacgccacgcgggatg aacctgtggcatttgtgctgccgggaacggcgtttcgtgtctctgccggtgtggcagccgaaatgacagagcgc- ggcctggccagaat gcaataacgggaggcgctgtggctgatttcgataacctgttcgatgctgccattgcccgcgccgatgaaacgat- acgcgggtacatggg aacgtcagccaccattacatccggtgagcagtcaggtgcggtgatacgtggtgtttttgatgaccctgaaaata- tcagctatgccggaca gggcgtgcgcgttgaaggctccagcccgtccctgtttgtccggactgatgaggtgcggcagctgcggcgtggag- acacgctgaccatc ggtgaggaaaatttctgggtagatcgggtttcgccggatgatggcggaagttgtcatactggcttggacggggc- gtaccgcctgccgtt aaccgtcgccgctgaaagggggatgtatggccataaaaggtcttgagcaggccgttgaaaacctcagccgtatc- agcaaaacggcggt gcctggtgccgccgcaatggccattaaccgcgttgcttcatccgcgatatcgcagtcggcgtcacaggttgccc- gtgagacaaaggtac gccggaaactggtaaaggaaagggccaggctgaaaagggccacggtcaaaaatccgcaggccagaatcaaagtt- aaccgggggga tttgcccgtaatcaagagggtaatgcgcgggttgtcctttcgcgccgcaggcgtcgtaaaaaggggcagcgttc- atccctgaaaggtg gcggcagcgtgcttgtggtgggtaaccgtcgtattcccggcgcgtttattcagcaactgaaaaatggccggtgg- catgtcatgcagcgtg tggctgggaaaaaccgttaccccattgatgtggtgaaaatcccgatggcggtgccgctgaccacggcgtttaaa- caaaatattgagcgg

atacggcgtgaacgtcttccgaaagagctgggctatgcgctgcagcatcaactgaggatggtaataaagcgatg- aaacatactgaactc cgtgcagccgtactggatgcactggagaagcatgacaccggggcgacgttttttgatggtcgccccgctgtttt- tgatgaggcggattttc cggcagttgccgtttatctcaccggcgctgaatacacgggcgaagagctggacagcgatacctggcaggcggag- ctgcatatcgaagt tttcctgcctgctcaggtgccggattcagagctggatgcgtggatggagtcccggatttatccggtgatgagcg- atatcccggcactgtca gatttgatcaccagtatggtggccagcggctatgactaccggcgcgacgatgatgcgggcttgtggagttcagc- cgatctgacttatgtc attacctatgaaatgtgaggacgctatgcctgtaccaaatcctacaatgccggtgaaaggtgccgggaccaccc- tgtgggtttataaggg gagcggtgacccttacgcgaatccgctttcagacgttgactggtcgcgtctggcaaaagttaaagacctgacgc- ccggcgaactgacc gctgagtcctatgacgacagctatctcgatgatgaagatgcagactggactgcgaccgggcaggggcagaaatc- tgccggagatacc agcttcacgctggcgtggatgcccggagagcaggggcagcaggcgctgctggcgtggtttaatgaaggcgatac- ccgtgcctataaa atccgcttcccgaacggcacggtcgatgtgttccgtggctgggtcagcagtatcggtaaggcggtgacggcgaa- ggaagtgatcaccc gcacggtgaaagtcaccaatgtgggacgtccgtcgatggcagaagatcgcagcacggtaacagcggcaaccggc- atgaccgtgacg cctgccagcacctcggtggtgaaagggcagagcaccacgctgaccgtggccttccagccggagggcgtaaccga- caagagctttcgt gcggtgtctgcggataaaacaaaagccaccgtgtcggtcagtggtatgaccatcaccgtgaacggcgttgctgc- aggcaaggtcaaca ttccggttgtatccggtaatggtgagtttgctgcggttgcagaaattaccgtcaccgccagttaatccggagag- tcagcgatgttcctgaaa accgaatcatttgaacataacggtgtgaccgtcacgctttctgaactgtcagccctgcagcgcattgagcatct- cgccctgatgaaacggc aggcagaacaggcggagtcagacagcaaccggaagtttactgtggaagacgccatcagaaccggcgcgtttctg- gtggcgatgtccc tgtggcataaccatccgcagaagacgcagatgccgtccatgaatgaagccgttaaacagattgagcaggaagtg- cttaccacctggcc cacggaggcaatttctcatgctgaaaacgtggtgtaccggctgtctggtatgtatgagtttgtggtgaataatg- cccctgaacagacagag gacgccgggcccgcagagcctgtttctgcgggaaagtgttcgacggtgagctgagttttgccctgaaactggcg- cgtgagatggggcg acccgactggcgtgccatgcttgccgggatgtcatccacggagtatgccgactggcaccgcttttacagtaccc- attattttcatgatgttct gctggatatgcacttttccgggctgacgtacaccgtgctcagcctgtttttcagcgatccggatatgcatccgc- tggatttcagtctgctgaa ccggcgcgaggctgacgaagagcctgaagatgatgtgctgatgcagaaagcggcagggcttgccggaggtgtcc- gctttggcccgg acgggaatgaagttatccccgcttccccggatgtggcggacatgacggaggatgacgtaatgctgatgacagta- tcagaagggatcgc aggaggagtccggtatggctgaaccggtaggcgatctggtcgttgatttgagtctggatgcggccagatttgac- gagcagatggccaga gtcaggcgtcatttttctggtacggaaagtgatgcgaaaaaaacagcggcagtcgttgaacagtcgctgagccg- acaggcgctggctg cacagaaagcggggatttccgtcgggcagtataaagccgccatgcgtatgctgcctgcacagttcaccgacgtg- gccacgcagcttgc aggcgggcaaagtccgtggctgatcctgctgcaacagggggggcaggtgaaggactccttcggcgggatgatcc- ccatgttcagggg gcttgccggtgcgatcaccctgccgatggtgggggccacctcgctggcggtggcgaccggtgcgctggcgtatg- cctggtatcaggg caactcaaccctgtccgatttcaacaaaacgctggtcctttccggcaatcaggcgggactgacggcagatcgta- tgctggtcctgtccag agccgggcaggcggcagggctgacgtttaaccagaccagcgagtcactcagcgcactggttaaggcgggggtaa- gcggtgaggctc agattgcgtccatcagccagagtgtggcgcgtttctcctctgcatccggcgtggaggtggacaaggtcgctgaa- gccttcgggaagctg accacagacccgacgtcggggctgacggcgatggctcgccagttccataacgtgtcggcggagcagattgcgta- tgttgctcagttgc agcgttccggcgatgaagccggggcattgcaggcggcgaacgaggccgcaacgaaagggtttgatgaccagacc- cgccgcctgaa agagaacatgggcacgctggagacctgggcagacaggactgcgcgggcattcaaatccatgtgggatgcggtgc- tggatattggtcgt cctgataccgcgcaggagatgctgattaaggcagaggctgcgtataagaaagcagacgacatctggaatctgcg- caaggatgattatttt gttaacgatgaagcgcgggcgcgttactgggatgatcgtgaaaaggcccgtcttgcgcttgaagccgcccgaaa- gaaggctgagcag cagactcaacaggacaaaaatgcgcagcagcagagcgataccgaagcgtcacggctgaaatataccgaagaggc- gcagaaggctta cgaacggctgcagacgccgctggagaaatataccgcccgtcaggaagaactgaacaaggcactgaaagacggga- aaatcctgcag gcggattacaacacgctgatggcggcggcgaaaaaggattatgaagcgacgctgaaaaagccgaaacagtccag- cgtgaaggtgtct gcgggcgatcgtcaggaagacagtgctcatgctgccctgctgacgcttcaggcagaactccggacgctggagaa- gcatgccggagc aaatgagaaaatcagccagcagcgccgggatttgtggaaggcggagagtcagttcgcggtactggaggaggcgg- cgcaacgtcgcc agctgtctgcacaggagaaatccctgctggcgcataaagatgagacgctggagtacaaacgccagctggctgca- cttggcgacaaggt tacgtatcaggagcgcctgaacgcgctggcgcagcaggcggataaattcgcacagcagcaacgggcaaaacggg- ccgccattgatg cgaaaagccgggggctgactgaccggcaggcagaacgggaagccacggaacagcgcctgaaggaacagtatggc- gataatccgct ggcgctgaataacgtcatgtcagagcagaaaaagacctgggcggctgaagaccagcttcgcgggaactggatgg- caggcctgaagt ccggctggagtgagtgggaagagagcgccacggacagtatgtcgcaggtaaaaagtgcagccacgcagaccttt- gatggtattgcac agaatatggcggcgatgctgaccggcagtgagcagaactggcgcagcttcacccgttccgtgctgtccatgatg- acagaaattctgctta agcaggcaatggtggggattgtcgggagtatcggcagcgccattggcggggctgttggtggcggcgcatccgcg- tcaggcggtaca gccattcaggccgctgcggcgaaattccattttgcaaccggaggatttacgggaaccggcggcaaatatgagcc- agcggggattgttca ccgtggtgagtttgtcttcacgaaggaggcaaccagccggattggcgtggggaatctttaccggctgatgcgcg- gctatgccaccggc ggttatgtcggtacaccgggcagcatggcagacagccggtcgcaggcgtccgggacgtttgagcagaataacca- tgtggtgattaaca acgacggcacgaacgggcagataggtccggctgctctgaaggcggtgtatgacatggcccgcaagggtgcccgt- gatgaaattcaga cacagatgcgtgatggtggcctgttctccggaggtggacgatgaagaccttccgctggaaagtgaaacccggta- tggatgtggcttcgg tcccttctgtaagaaaggtgcgctttggtgatggctattctcagcgagcgcctgccgggctgaatgccaacctg- aaaacgtacagcgtga cgctttctgtcccccgtgaggaggccacggtactggagtcgtttctggaagagcacgggggctggaaatccttt- ctgtggacgccgcctt atgagtggcggcagataaaggtgacctgcgcaaaatggtcgtcgcgggtcagtatgctgcgtgttgagttcagc- gcagagtttgaacag gtggtgaactgatgcaggatatccggcaggaaacactgaatgaatgcacccgtgcggagcagtcggccagcgtg- gtgctctgggaaa tcgacctgacagaggtcggtggagaacgttattttttctgtaatgagcagaacgaaaaaggtgagccggtcacc- tggcaggggcgaca gtatcagccgtatcccattcaggggagcggttttgaactgaatggcaaaggcaccagtacgcgccccacgctga- cggtttctaacctgta cggtatggtcaccgggatggcggaagatatgcagagtctggtcggcggaacggtggtccggcgtaaggtttacg- cccgttttctggatg cggtgaacttcgtcaacggaaacagttacgccgatccggagcaggaggtgatcagccgctggcgcattgagcag- tgcagcgaactga gcgcggtgagtgcctcctttgtactgtccacgccgacggaaacggatggcgctgtttttccgggacgtatcatg- ctggccaacacctgca cctggacctatcgcggtgacgagtgcggttatagcggtccggctgtcgcggatgaatatgaccagccaacgtcc- gatatcacgaaggat aaatgcagcaaatgcctgagcggttgtaagttccgcaataacgtcggcaactttggcggcttcctttccattaa- caaactttcgcagtaaat cccatgacacagacagaatcagcgattctggcgcacgcccggcgatgtgcgccagcggagtcgtgcggcttcgt- ggtaagcacgccg gagggggaaagatatttcccctgcgtgaatatctccggtgagccggaggctatttccgtatgtcgccggaagac- tggctgcaggcagaa atgcagggtgagattgtggcgctggtccacagccaccccggtggtctgccctggctgagtgaggccgaccggcg- gctgcaggtgcag agtgatttgccgtggtggctggtctgccgggggacgattcataagttccgctgtgtgccgcatctcaccgggcg- gcgctttgagcacggt gtgacggactgttacacactgttccgggatgcttatcatctggcggggattgagatgccggactttcatcgtga- ggatgactggtggcgta acggccagaatctctatctggataatctggaggcgacggggctgtatcaggtgccgttgtcagcggcacagccg- ggcgatgtgctgct gtgctgttttggttcatcagtgccgaatcacgccgcaatttactgcggcgacggcgagctgctgcaccatattc- ctgaacaactgagcaaa cgagagaggtacaccgacaaatggcagcgacgcacacactccctctggcgtcaccgggcatggcgcgcatctgc- ctttacggggattt acaacgatttggtcgccgcatcgaccttcgtgtgaaaacgggggctgaagccatccgggcactggccacacagc- tcccggcgtttcgt cagaaactgagcgacggctggtatcaggtacggattgccgggcgggacgtcagcacgtccgggttaacggcgca- gttacatgagact ctgcctgatggcgctgtaattcatattgttcccagagtcgccggggccaagtcaggtggcgtattccagattgt- cctgggggctgccgcc attgccggatcattctttaccgccggagccacccttgcagcatggggggcagccattggggccggtggtatgac- cggcatcctgttttct ctcggtgccagtatggtgctcggtggtgtggcgcagatgctggcaccgaaagccagaactccccgtatacagac- aacggataacggta agcagaacacctatttctcctcactggataacatggttgcccagggcaatgttctgcctgttctgtacggggaa- atgcgcgtggggtcacg cgtggtttctcaggagatcagcacggcagacgaaggggacggtggtcaggttgtggtgattggtcgctgatgca- aaatgttttatgtgaa accgcctgcgggcggttttgtcatttatggagcgtgaggaatgggtaaaggaagcagtaaggggcataccccgc- gcgaagcgaagga caacctgaagtccacgcagttgctgagtgtgatcgatgccatcagcgaagggccgattgaaggtccggtggatg- gcttaaaaagcgtg ctgctgaacagtacgccggtgctggacactgaggggaataccaacatatccggtgtcacggtggtgttccgggc- tggtgagcaggagc agactccgccggagggatttgaatcctccggctccgagacggtgctgggtacggaagtgaaatatgacacgccg- atcacccgcaccat tacgtctgcaaacatcgaccgtctgcgctttaccttcggtgtacaggcactggtggaaaccacctcaaagggtg- acaggaatccgtcgg

aagtccgcctgctggttcagatacaacgtaacggtggctgggtgacggaaaaagacatcaccattaagggcaaa- accacctcgcagta tctggcctcggtggtgatgggtaacctgccgccgcgcccgtttaatatccggatgcgcaggatgacgccggaca- gcaccacagacca gctgcagaacaaaacgctctggtcgtcatacactgaaatcatcgatgtgaaacagtgctacccgaacacggcac- tggtcggcgtgcag gtggactcggagcagttcggcagccagcaggtgagccgtaattatcatctgcgcgggcgtattctgcaggtgcc- gtcgaactataaccc gcagacgcggcaatacagcggtatctgggacggaacgtttaaaccggcatacagcaacaacatggcctggtgtc- tgtgggatatgctg acccatccgcgctacggcatggggaaacgtcttggtgcggcggatgtggataaatgggcgctgtatgtcatcgg- ccagtactgcgacc agtcagtgccggacggctttggcggcacggagccgcgcatcacctgtaatgcgtacctgaccacacagcgtaag- gcgtgggatgtgct cagcgatttctgctcggcgatgcgctgtatgccggtatggaacgggcagacgctgacgttcgtgcaggaccgac- cgtcggataagacg tggacctataaccgcagtaatgtggtgatgccggatgatggcgcgccgttccgctacagcttcagcgccctgaa- ggaccgccataatgc cgttgaggtgaactggattgacccgaacaacggctgggagacggcgacagagcttgttgaagatacgcaggcca- ttgcccgttacggt cgtaatgttacgaagatggatgcctttggctgtaccagccgggggcaggcacaccgcgccgggctgtggctgat- taaaacagaactgc tggaaacgcagaccgtggatttcagcgtcggcgcagaagggcttcgccatgtaccgggcgatgttattgaaatc- tgcgatgatgactatg ccggtatcagcaccggtggtcgtgtgctggcggtgaacagccagacccggacgctgacgctcgaccgtgaaatc- acgctgccatcctc cggtaccgcgctgataagcctggttgacggaagtggcaatccggtcagcgtggaggttcagtccgtcaccgacg- gcgtgaaggtaaa agtgagccgtgttcctgacggtgttgctgaatacagcgtatgggagctgaagctgccgacgctgcgccagcgac- tgttccgctgcgtga gtatccgtgagaacgacgacggcacgtatgccatcaccgccgtgcagcatgtgccggaaaaagaggccatcgtg- gataacggggcg cactttgacggcgaacagagtggcacggtgaatggtgtcacgccgccagcggtgcagcacctgaccgcagaagt- cactgcagacag cggggaatatcaggtgctggcgcgatgggacacaccgaaggtggtgaagggcgtgagtttcctgctccgtctga- ccgtaacagcgga cgacggcagtgagcggctggtcagcacggcccggacgacggaaaccacataccgcttcacgcaactggcgctgg- ggaactacagg ctgacagtccgggcggtaaatgcgtgggggcagcagggcgatccggcgtcggtatcgttccggattgccgcacc- ggcagcaccgtc gaggattgagctgacgccgggctattttcagataaccgccacgccgcatcttgccgtttatgacccgacggtac- agtttgagttctggttct cggaaaagcagattgcggatatcagacaggttgaaaccagcacgcgttatcttggtacggcgctgtactggata- gccgccagtatcaat atcaaaccgggccatgattattacttttatatccgcagtgtgaacaccgttggcaaatcggcattcgtggaggc- cgtcggtcgggcgagc gatgatgcggaaggttacctggattttttcaaaggcaagataaccgaatcccatctcggcaaggagctgctgga- aaaagtcgagctgac ggaggataacgccagcagactggaggagttttcgaaagagtggaaggatgccagtgataagtggaatgccatgt- gggctgtcaaaatt gagcagaccaaagacggcaaacattatgtcgcgggtattggcctcagcatggaggacacggaggaaggcaaact- gagccagtttctg gttgccgccaatcgtatcgcatttattgacccggcaaacgggaatgaaacgccgatgtttgtggcgcagggcaa- ccagatattcatgaac gacgtgttcctgaagcgcctgacggcccccaccattaccagcggcggcaatcctccggccttttccctgacacc- ggacggaaagctga ccgctaaaaatgcggatatcagtggcagtgtgaatgcgaactccgggacgctcagtaatgtgacgatagctgaa- aactgtacgataaac ggtacgctgagggcggaaaaaatcgtcggggacattgtaaaggcggcgagcgcggcttttccgcgccagcgtga- aagcagtgtgga ctggccgtcaggtacccgtactgtcaccgtgaccgatgaccatccttttgatcgccagatagtggtgcttccgc- tgacgtttcgcggaagt aagcgtactgtcagcggcaggacaacgtattcgatgtgttatctgaaagtactgatgaacggtgcggtgattta- tgatggcgcggcgaac gaggcggtacaggtgttctcccgtattgttgacatgccagcgggtcggggaaacgtgatcctgacgttcacgct- tacgtccacacggcat tcggcagatattccgccgtatacgtttgccagcgatgtgcaggttatggtgattaagaaacaggcgctgggcat- cagcgtggtctgagtgt gttacagaggttcgtccgggaacgggcgttttattataaaacagtgagaggtgaacgatgcgtaatgtgtgtat- tgccgttgctgtctttgcc gcacttgcggtgacagtcactccggcccgtgcggaaggtggacatggtacgtttacggtgggctattttcaagt- gaaaccgggtacattg ccgtcgttgtcgggcggggataccggtgtgagtcatctgaaagggattaacgtgaagtaccgttatgagctgac- ggacagtgtgggggt gatggcttccctggggttcgccgcgtcgaaaaagagcagcacagtgatgaccggggaggatacgtttcactatg- agagcctgcgtgga cgttatgtgagcgtgatggccggaccggttttacaaatcagtaagcaggtcagtgcgtacgccatggccggagt- ggctcacagtcggtg gtccggcagtacaatggattaccgtaagacggaaatcactcccgggtatatgaaagagacgaccactgccaggg- acgaaagtgcaat gcggcatacctcagtggcgtggagtgcaggtatacagattaatccggcagcgtccgtcgttgttgatattgctt- atgaaggctccggcagt ggcgactggcgtactgacggattcatcgttggggtcggttataaattctgattagccaggtaacacagtgttat- gacagcccgccggaac cggtgggcttttttgtggggtgaatatggcagtaaagatttcaggagtcctgaaagacggcacaggaaaaccgg- tacagaactgcacca ttcagctgaaagccagacgtaacagcaccacggtggtggtgaacacggtgggctcagagaatccggatgaagcc- gggcgttacagca tggatgtggagtacggtcagtacagtgtcatcctgcaggttgacggttttccaccatcgcacgccgggaccatc- accgtgtatgaagattc acaaccggggacgctgaatgattttctctgtgccatgacggaggatgatgcccggccggaggtgctgcgtcgtc- ttgaactgatggtgg aagaggtggcgcgtaacgcgtccgtggtggcacagagtacggcagacgcgaagaaatcagccggcgatgccagt- gcatcagctgct caggtcgcggcccttgtgactgatgcaactgactcagcacgcgccgccagcacgtccgccggacaggctgcatc- gtcagctcaggaa gcgtcctccggcgcagaagcggcatcagcaaaggccactgaagcggaaaaaagtgccgcagccgcagagtcctc- aaaaaacgcgg cggccaccagtgccggtgcggcgaaaacgtcagaaacgaatgctgcagcgtcacaacaatcagccgccacgtct- gcctccaccgcg gccacgaaagcgtcagaggccgccacttcagcacgagatgcggtggcctcaaaagaggcagcaaaatcatcaga- aacgaacgcatc atcaagtgccggtcgtgcagcttcctcggcaacggcggcagaaaattctgccagggcggcaaaaacgtccgaga- cgaatgccaggtc atctgaaacagcagcggaacggagcgcctctgccgcggcagacgcaaaaacagcggcggcggggagtgcgtcaa- cggcatccac gaaggcgacagaggctgcgggaagtgcggtatcagcatcgcagagcaaaagtgcggcagaagcggcggcaatac- gtgcaaaaaat tcggcaaaacgtgcagaagatatagcttcagctgtcgcgcttgaggatgcggacacaacgagaaaggggatagt- gcagctcagcagt gcaaccaacagcacgtctgaaacgcttgctgcaacgccaaaggcggttaaggtggtaatggatgaaacgaacag- aaaagcccactgg acagtccggcactgaccggaacgccaacagcaccaaccgcgctcaggggaacaaacaatacccagattgcgaac- accgcttttgtac tggccgcgattgcagatgttatcgacgcgtcacctgacgcactgaatacgctgaatgaactggccgcagcgctc- gggaatgatccagat tttgctaccaccatgactaacgcgcttgcgggtaaacaaccgaagaatgcgacactgacggcgctggcagggct- ttccacggcgaaaa ataaattaccgtattttgcggaaaatgatgccgccagcctgactgaactgactcaggttggcagggatattctg- gcaaaaaattccgttgca gatgttcttgaataccttggggccggtgagaattcggcctttccggcaggtgcgccgatcccgtggccatcaga- tatcgttccgtctggct acgtcctgatgcaggggcaggcgtttgacaaatcagcctacccaaaacttgctgtcgcgtatccatcgggtgtg- cttcctgatatgcgag gctggacaatcaaggggaaacccgccagcggtcgtgctgtattgtctcaggaacaggatggaattaagtcgcac- acccacagtgccag tgcatccggtacggatttggggacgaaaaccacatcgtcgtttgattacgggacgaaaacaacaggcagtttcg- attacggcaccaaatc gacgaataacacgggggctcatgctcacagtctgagcggttcaacaggggccgcgggtgctcatgcccacacaa- gtggtttaaggatg aacagttctggctggagtcagtatggaacagcaaccattacaggaagtttatccacagttaaaggaaccagcac- acagggtattgcttatt tatcgaaaacggacagtcagggcagccacagtcactcattgtccggtacagccgtgagtgccggtgcacatgcg- catacagttggtatt ggtgcgcaccagcatccggttgttatcggtgctcatgcccattctttcagtattggttcacacggacacaccat- caccgttaacgctgcgg gtaacgcggaaaacaccgtcaaaaacattgcatttaactatattgtgaggcttgcataatggcattcagaatga- gtgaacaaccacggac cataaaaatttataatctgctggccggaactaatgaatttattggtgaaggtgacgcatatattccgcctcata- ccggtctgcctgcaaacag taccgatattgcaccgccagatattccggctggctttgtggctgttttcaacagtgatgaggcatcgtggcatc- tcgttgaagaccatcggg gtaaaaccgtctatgacgtggcttccggcgacgcgttatttatttctgaactcggtccgttaccggaaaatttt- acctggttatcgccgggag gggaatatcagaagtggaacggcacagcctgggtgaaggatacggaagcagaaaaactgttccggatccgggag- gcggaagaaac aaaaaaaagcctgatgcaggtagccagtgagcatattgcgccgcttcaggatgctgcagatctggaaattgcaa- cgaaggaagaaacc tcgttgctggaagcctggaagaagtatcgggtgttgctgaaccgtgttgatacatcaactgcacctgatattga- gtggcctgctgtccctgt tatggagtaatcgttttgtgatatgccgcagaaacgttgtatgaaataacgttctgcggttagttagtatattg- taaagctgagtattggtttattt ggcgattattatcttcaggagaataatggaagttctatgactcaattgttcatagtgtttacatcaccgccaat- tgcttttaagactgaacgcat gaaatatggtttttcgtcatgttttgagtctgctgttgatatttctaaagtcggttttttttcttcgttttctc- taactattttccatgaaatacatttttga ttattatttgaatcaattccaattacctgaagtctttcatctataattggcattgtatgtattggtttattgga- gtagatgcttgcttttctgagccata gctctgatatccaaatgaagccataggcatttgttattttggctctgtcagctgcataacgccaaaaaatatat- ttatctgcttgatcttcaaatg ttgtattgattaaatcaattggatggaattgtttatcataaaaaattaatgtttgaatgtgataaccgtccttt- aaaaaagtcgtttctgcaagctt ggctgtatagtcaactaactcttctgtcgaagtgatatttttaggcttatctaccagttttagacgctctttaa- tatcttcaggaattattttattgtc atattgtatcatgctaaatgacaatttgcttatggagtaatcttttaattttaaataagttattctcctggctt- catcaaataaagagtcgaatgatg ttggcgaaatcacatcgtcacccattggattgtttatttgtatgccaagagagttacagcagttatacattctg-

ccatagattatagctaaggc atgtaataattcgtaatcttttagcgtattagcgacccatcgtctttctgatttaataatagatgattcagtta- aatatgaaggtaatttcttttgtgc aagtctgactaacttttttataccaatgtttaacatactttcatttgtaataaactcaatgtcattttcttcaa- tgtaagatgaaataagagtagcctt tgcctcgctatacatttctaaatcgccttgtttttctatcgtattgcgagaatttttagcccaagccattaatg- gatcatttttccatttttcaataac attattgttataccaaatgtcatatcctataatctggtttttgtttttttgaataataaatgttactgttcttg- cggtttggaggaattgattcaaattca agcgaaataattcagggtcaaaatatgtatcaatgcagcatttgagcaagtgcgataaatctttaagtcttctt- tcccatggttttttagtcataa aactctccattttgataggttgcatgctagatgctgatatattttagaggtgataaaattaactgcttaactgt- caatgtaatacaagttgtttgat ctttgcaatgattcttatcagaaaccatatagtaaattagttacacaggaaatttttaatattattattatcat- tcattatgtattaaaattagagttgt ggcttggctctgctaacacgttgctcataggagatatggtagagccgcagacacgtcgtatgcaggaacgtgct- gcggctggctggtga acttccgatagtgcgggtgttgaatgatttccagttgctaccgattttacatattttttgcatgagagaatttg- taccacctcccaccgaccatc tatgactgtacgccactgtccctaggactgctatgtgccggagcggacattacaaacgtccttctcggtgcatg- ccactgttgccaatgac ctgcctaggaattggttagcaagttactaccggattttgtaaaaacagccctcctcatataaaaagtattcgtt- cacttccgataagcgtcgta attttctatctttcatcatattctagatccctctgaaaaaatcttccgagtttgctaggcactgatacataact- cttttccaataattggggaagtc attcaaatctataataggtttcagatttgcttcaataaattctgactgtagctgctgaaacgttgcggttgaac- tatatttccttataacttttacga aagagtttctttgagtaatcacttcactcaagtgcttccctgcctccaaacgatacctgttagcaatatttaat- agcttgaaatgatgaagagct ctgtgtttgtcttcctgcctccagttcgccgggcattcaacataaaaactgatagcacccggagttccggaaac- gaaatttgcatataccca ttgctcacgaaaaaaaatgtccttgtcgatatagggatgaatcgcttggtgtacctcatctactgcgaaaactt- gacctttctctcccatattg cagtcgcggcacgatggaactaaattaataggcatcaccgaaaattcaggataatgtgcaataggaagaaaatg- atctatattttttgtctgt cctatatcaccacaaaatggacatttttcacctgatgaaacaagcatgtcatcgtaatatgttctagcgggttt- gtttttatctcggagattatttt cataaagcttttctaatttaacctttgtcaggttaccaactactaaggttgtaggctcaagagggtgtgtcctg- tcgtaggtaaataactgacc tgtcgagcttaatattctatattgttgttctttctgcaaaaaagtggggaagtgagtaatgaaattatttctaa- catttatctgcatcataccttcc gagcatttattaagcatttcgctataagttctcgctggaagaggtagttttttcattgtactttaccttcatct- ctgttcattatcatcgcttttaaaa cggttcgaccttctaatcctatctgaccattataattttttagaatggtttcataagaaagctctgaatcaacg- gactgcgataataagtggtgg tatccagaatttgtcacttcaagtaaaaacacctcacgagttaaaacacctaagttctcaccgaatgtctcaat- atccggacggataatattta ttgcttctcttgaccgtaggactttccacatgcaggattttggaacctcttgcagtactactggggaatgagtt- gcaattattgctacaccattg cgtgcatcgagtaagtcgcttaatgttcgtaaaaaagcagagagcaaaggtggatgcagatgaacctctggttc- atcgaataaaactaat gacttttcgccaacgacatctactaatcttgtgatagtaaataaaacaattgcatgtccagagctcattcgaag- cagatatttctggatattgtc ataaaacaatttagtgaatttatcatcgtccacttgaatctgtggttcattacgtcttaactcttcatatttag- aaatgaggctgatgagttccata tttgaaaagttttcatcactacttagttttttgatagcttcaagccagagttgtctttttctatctactctcat- acaaccaataaatgctgaaatgaa ttctaagcggagatcgcctagtgattttaaactattgctggcagcattcttgagtccaatataaaagtattgtg- taccttttgctgggtcaggtt gttctttaggaggagtaaaaggatcaaatgcactaaacgaaactgaaacaagcgatcgaaaatatccattggga- ttcttgactcgataag tctattattttcagagaaaaaatattcattgttttctgggttggtgattgcaccaatcattccattcaaaattg- ttgttttaccacacccattccgcc cgataaaagcatgaatgttcgtgctgggcatagaattaaccgtcacctcaaaaggtatagttaaatcactgaat- ccgggagcactttttctat taaatgaaaagtggaaatctgacaattctggcaaaccatttaacacacgtgcgaactgtccatgaatttctgaa- agagttacccctctaagt aatgaggtgttaaggacgctttcattttcaatgtcggctaatcgatttggccatactactaaatcctgaatagc- tttaagaaggttatgtttaaa accatcgcttaatttgctgagattaacatagtagtcaatgctttcacctaaggaaaaaaacatttcagggagtt- gactgaattttttatctattaa tgaataagtgcttacttcttctttttgacctacaaaaccaattttaacatttccgatatcgcatttttcaccat- gctcatcaaagacagtaagataa aacattgtaacaaaggaatagtcattccaaccatctgctcgtaggaatgccttatttttttctactgcaggaat- atacccgcctctttcaataac actaaactccaacatatagtaacccttaattttattaaaataaccgcaatttatttggcggcaacacaggatct- ctcttttaagttactctctatta catacgttttccatctaaaaattagtagtattgaacttaacggggcatcgtattgtagttttccatatttagct- ttctgcttccttttggataaccca ctgttattcatgttgcatggtgcactgtttataccaacgatatagtctattaatgcatatatagtatcgccgaa- cgattagctcttcaggcttctg aagaagcgtttcaagtactaataagccgatagatagccacggacttcgtagccatttttcataagtgttaactt- ccgctcctcgctcataaca gacattcactacagttatggcggaaaggtatgcatgctgggtgtggggaagtcgtgaaagaaaagaagtcagct- gcgtcgtttgacatca ctgctatcttcttactggttatgcaggtcgtagtgggtggcacacaaagctttgcactggattgcgaggctttg- tgcttctctggagtgcgac aggtttgatgacaaaaaattagcgcaagaagacaaaaatcaccttgcgctaatgctctgttacaggtcactaat- accatctaagtagttgatt catagtgactgcatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatatt- gatatttatatcattttacgtttctcgtt cagcttttttatactaagttggcattataaaaaagcattgcttatcaatttgttgcaacgaacaggtcactatc- agtcaaaataaaatcattattt gatttcaattttgtcccactccctgcctctgtcatcacgatactgtgatgccatggtgtccgacttatgcccga- gaagatgttgagcaaactta tcgcttatctgcttctcatagagtcttgcagacaaactgcgcaactcgtgaaaggtaggcggatccccttcgaa- ggaaagacctgatgcttt tcgtgcgcgcataaaataccttgatactgtgccggatgaaagcggttcgcgacgagtagatgcaattatggttt- ctccgccaagaatctctt tgcatttatcaagtgtttccttcattgatattccgagagcatcaatatgcaatgctgttgggatggcaattttt- acgcctgttttgctttgctcgac ataaagatatccatctacgatatcagaccacttcatttcgcataaatcaccaactcgttgcccggtaacaacag- ccagttccattgcaagtct gagccaacatggtgatgattctgctgcttgataaattttcaggtattcgtcagccgtaagtcttgatctcctta- cctctgattttgctgcgcgag tggcagcgacatggtttgttgttatatggccttcagctattgcctctcggaatgcatcgctcagtgttgatctg- attaacttggctgacgccgc cttgccctcgtctatgtatccattgagcattgccgcaatttcttttgtggtgatgtcttcaagtggagcatcag- gcagacccctccttattgcttt aattttgctcatgtaatttatgagtgtcttctgcttgattcctctgctggccaggattttttcgtagcgatcaa- gccatgaatgtaacgtaacgga attatcactgttgattctcgctgtcagaggcttgtgtttgtgtcctgaaaataactcaatgttggcctgtatag- cttcagtgattgcgattcgcct gtctctgcctaatccaaactctttacccgtccttgggtccctgtagcagtaatatccattgtttcttatataaa- ggttagggggtaaatcccggc gctcatgacttcgccttcttcccatttctgatcctcttcaaaaggccacctgttactggtcgatttaagtcaac- ctttaccgctgattcgtggaa cagatactctcttccatccttaaccggaggtgggaatatcctgcattcccgaacccatgacgaactgtttcaag- gcttcttggacgtcgct ggcgtgcgttccactcctgaagtgtcaagtacatcgcaaagtctccgcaattacacgcaagaaaaaaccgccat- caggcggcttggtgtt ctttcagttcttcaattcgaatattggttacgtctgcatgtgctatctgcgcccatatcatccagtggtcgtag- cagtcgttgatgttctccgctt cgataactctgttgaatggctctccattccattctcctgtgactcggaagtgcatttatcatctccataaaaca- aaacccgccgtagcgagtt cagataaaataaatccccgcgagtgcgaggattgttatgtaatattgggtttaatcatctatatgttttgtaca- gagagggcaagtatcgtttc caccgtactcgtgataataattttgcacggtatcagtcatttctcgcacattgcagaatggggatttgtcttca- ttagacttataaaccttcatg gaatatttgtatgccgactctatatctataccttcatctacataaacaccttcgtgatgtctgcatggagacaa- gacaccggatctgcacaac attgataacgcccaatctttttgctcagactctaactcattgatactcatttataaactccttgcaatgtatgt- cgtttcagctaaacggtatcag caatgtttatgtaaagaaacagtaagataatactcaacccgatgtttgagtacggtcatcatctgacactacag- actctggcatcgctgtga agacgacgcgaaattcagcattttcacaagcgttatcttttacaaaaccgatctcactctcctttgatgcgaat- gccagcgtcagacatcata tgcagatactcacctgcatcctgaacccattgacctccaaccccgtaatagcgatgcgtaatgatgtcgatagt- tactaacgggtcttgttc gattaactgccgcagaaactcttccaggtcaccagtgcagtgcttgataacaggagtcttcccaggatggcgaa- caacaagaaactggtt tccgtcttcacggacttcgttgctttccagtttagcaatacgcttactcccatccgagataacaccttcgtaat- actcacgctgctcgttgagtt ttgattttgctgtttcaagctcaacacgcagtttccctactgttagcgcaatatcctcgttctcctggtcgcgg- cgtttgatgtattgctggtttct ttcccgttcatccagcagttccagcacaatcgatggtgttaccaattcatggaaaaggtctgcgtcaaatcccc- agtcgtcatgcattgcct gctctgccgcttcacgcagtgcctgagagttaatttcgctcacttcgaacctctctgtttactgataagttcca- gatcctcctggcaacttgca caagtccgacaaccctgaacgaccaggcgtcttcgttcatctatcggatcgccacactcacaacaatgagtggc- agatatagcctggtg gttcaggcggcgcatttttattgctgtgttgcgctgtaattcttctatttctgatgctgaatcaatgatgtctg- ccatctttcattaatccctgaact gttggttaatacgcttgagggtgaatgcgaataataaaaaaggagcctgtagctccctgatgctttttgctttt- catgttcatcgttccttaaaga cgccgtttaacatgccgattgccaggcttaaatgagtcggtgtgaatcccatcagcgttaccgtttcgcggtgc- ttcttcagtacgctacgg caaatgtcatcgacgtttttatccggaaactgctgtctggctttttttgatttcagaattagcctgacgggcaa- tgctgcgaagggcgttttcct gctgaggtgtcattgaacaagtcccatgtcggcaagcataagcacacagaatatgaagcccgctgccagaaaaa- tgcattccgtggttgt catacctggtttctctcatctgcttctgctttcgccaccatcatttccagcttttgtgaaagggatgcggctaa- cgtatgaaattcttcgtctgttt ctactggtattggcacaaacctgattccaatttgagcaaggctatgtgccatctcgatactcgttcttaactca- acagaagatgctttgtgcat acagcccctcgtttattatttatctcctcagccagccgctgtgctttcagtggatttcggataacagaaaggcc- gggaaatacccagcctcg

ctttgtaacggagtagacgaaagtgattgcgcctacccggatattatcgtgaggatgcgtcatcgccattgctc- cccaaatacaaaaccaa tttcagccagtgcctcgtccattttttcgatgaactccggcacgatctcgtcaaaactcgccatgtacttttca- tcccgctcaatcacgacata atgcaggccttcacgcttcatacgcgggtcatagttggcaaagtaccaggcattttttcgcgtcacccacatgc- tgtactgcacctgggcc atgtaagctgactttatggcctcgaaaccaccgagccggaacttcatgaaatcccgggaggtaaacgggcattt- cagttcaaggccgttg ccgtcactgcataaaccatcgggagagcaggcggtacgcatactttcgtcgcgatagatgatcggggattcagt- aacattcacgccgga agtgaattcaaacagggttctggcgtcgttctcgtactgttttccccaggccagtgctttagcgttaacttccg- gagccacaccggtgcaaa cctcagcaagcagggtgtggaagtaggacattttcatgtcaggccacttctttccggagcggggttttgctatc- acgttgtgaacttctgaa gcggtgatgacgccgagccgtaatttgtgccacgcatcatccccctgttcgacagctctcacatcgatcccggt- acgctgcaggataatg tccggtgtcatgctgccaccttctgctctgcggctttctgtttcaggaatccaagagcttttactgcttcggcc- tgtgtcagttctgacgatgc acgaatgtcgcggcgaaatatctgggaacagagcggcaataagtcgtcatcccatgttttatccagggcgatca- gcagagtgttaatctc ctgcatggtttcatcgttaaccggagtgatgtcgcgttccggctgacgttctgcagtgtatgcagtattttcga- caatgcgctcggcttcatc cttgtcatagataccagcaaatccgaaggccagacgggcacactgaatcatggctttatgacgtaacatccgtt- tgggatgcgactgcca cggccccgtgatttctctgccttcgcgagttttgaatggttcgcggcggcattcatccatccattcggtaacgc- agatcggatgattacggt ccttgcggtaaatccggcatgtacaggattcattgtcctgctcaaagtccatgccatcaaactgctggttttca- ttgatgatgcgggaccag ccatcaacgcccaccaccggaacgatgccattctgcttatcaggaaaggcgtaaatttctttcgtccacggatt- aaggccgtactggttgg caacgatcagtaatgcgatgaactgcgcatcgctggcatcacctttaaatgccgtctggcgaagagtggtgatc- agttcctgtgggtcga cagaatccatgccgacacgttcagccagcttcccagccagcgttgcgagtgcagtactcattcgttttatacct- ctgaatcaatatcaacct ggtggtgagcaatggtttcaaccatgtaccggatgtgttctgccatgcgctcctgaaactcaacatcgtcatca- aacgcacgggtaatgga ttttttgctggccccgtggcgttgcaaatgatcgatgcatagcgattcaaacaggtgctggggcaggccttttt- ccatgtcgtctgccagttc tgcctctttctcttcacgggcgagctgctggtagtgacgcgcccagctctgagcctcaagacgatcctgaatgt- aataagcgttcatggct gaactcctgaaatagctgtgaaaatatcgcccgcgaaatgccgggctgattaggaaaacaggaaagggggttag- tgaatgcttttgcttg atctcagtttcagtattaatatccattttttataagcgtcgacggcttcacgaaacatcttttcatcgccaata- aaagtggcgatagtgaatttag tctggatagccataagtgtttgatccattctttgggactcctggctgattaagtatgtcgataaggcgtttcca- tccgtcacgtaatttacgggt gattcgttcaagtaaagattcggaagggcagccagcaacaggccaccctgcaatggcatattgcatggtgtgct- ccttatttatacataac gaaaaacgcctcgagtgaagcgttattggtatgcggtaaaaccgcactcaggcggccttgatagtcatatcatc- tgaatcaaatattcctg atgtatcgatatcggtaattcttattccttcgctaccatccattggaggccatccttcctgaccatttccatca- ttccagtcgaactcacacaca acaccatatgcatttaagtcgcttgaaattgctataagcagagcatgttgcgccagcatgattaatacagcatt- taatacagagccgtgtttat tgagtcggtattcagagtctgaccagaaattattaatctggtgaagtttttcctctgtcattacgtcatggtcg- atttcaatttctattgatgctttc cagtcgtaatcaatgatgtattttttgatgtttgacatctgttcatatcctcacagataaaaaatcgccctcac- actggagggcaaagaagatt tccaataatcagaacaagtcggctcctgtttagttacgagcgacattgctccgtgtattcactcgttggaatga- atacacagtgcagtgtttat tctgttatttatgccaaaaataaaggccactatcaggcagctttgttgttctgtttaccaagttctctggcaat- cattgccgtcgttcgtattgcc catttatcgacatatttcccatcttccattacaggaaacatttcttcaggcttaaccatgcattccgattgcag- cttgcatccattgcatcgcttg aattgtccacaccattgatttttatcaatagtcgtagtcatacggatagtcctggtattgttccatcacatcct- gaggatgctcttcgaactcttc aaattcttcttccatatatcaccttaaatagtggattgcggtagtaaagattgtgcctgtcttttaaccacatc- aggctcggtggttctcgtgtac ccctacagcgagaaatcggataaactattacaacccctacagtttgatgagtatagaaatggatccactcgtta- ttctcggacgagtgttca gtaatgaacctctggagagaaccatgtatatgatcgttatctgggttggacttctgcttttaagcccagataac- tggcctgaatatgttaatga gagaatcggtattcctcatgtgtggcatgttttcgtctttgctcttgcattttcgctagcaattaatgtgcatc- gattatcagctattgccagcgc cagatataagcgatttaagctaagaaaacgcattaagatgcaaaacgataaagtgcgatcagtaattcaaaacc- ttacagaagagcaatc tatggttttgtgcgcagcccttaatgaaggcaggaagtatgtggttacatcaaaacaattcccatacattagtg- agttgattgagcttggtgt gttgaacaaaactttttcccgatggaatggaaagcatatattattccctattgaggatatttactggactgaat- tagttgccagctatgatccat ataatattgagataaagccaaggccaatatctaagtaactagataagaggaatcgattttcccttaattttctg- gcgtccactgcatgttatgc cgcgttcgccaggcttgctgtaccatgtgcgctgattcttgcgctcaatacgttgcaggttgctttcaatctgt- ttgtggtattcagccagcac tgtaaggtctatcggatttagtgcgctttctactcgtgatttcggtttgcgattcagcgagagaatagggcggt- taactggttttgcgcttacc ccaaccaacaggggatttgctgctttccattgagcctgtttctctgcgcgacgttcgcggcggcgtgtttgtgc- atccatctggattctcctg tcagttagctttggtggtgtgtggcagttgtagtcctgaacgaaaaccccccgcgattggcacattggcagcta- atccggaatcgcactta cggccaatgcttcgtttcgtatcacacaccccaaagccttctgctttgaatgctgcccttcttcagggcttaat- ttttaagagcgtcaccttcat ggtggtcagtgcgtcctgctgatgtgctcagtatcaccgccagtggtatttatgtcaacaccgccagagataat- ttatcaccgcagatggtt atctgtatgttttttatatgaatttattttttgcaggggggcattgtttggtaggtgagagatctgaattgcta- tgtttagtgagttgtatctatttattt ttcaataaatacaattggttatgtgttttgggggcgatcgtgaggcaaagaaaacccggcgctgaggccgggtt- attcttgttctctggtca aattatatagttggaaaacaaggatgcatatatgaatgaacgatgcagaggcaatgccgatggcgatagtgggt- atcatgtagccgcttat gctggaaagaagcaataacccgcagaaaaacaaagctccaagctcaacaaaactaagggcatagacaataacta- ccgatgtcatatac ccatactctctaatcttggccagtcggcgcgttctgcttccgattagaaacgtcaaggcagcaatcaggattgc- aatcatggttcctgcatat gatgacaatgtcgccccaagaccatctctatgagctgaaaaagaaacaccaggaatgtagtggcggaaaaggag- atagcaaatgctta cgataacgtaaggaattattactatgtaaacaccaggcatgattctgttccgcataattactcctgataattaa- tccttaactttgcccacctgc cttttaaaacattccagtatatcacttttcattcttgcgtagcaatatgccatctcttcagctatctcagcatt- ggtgaccttgttcagaggcgct gagagatggcctttttctgatagataatgttctgttaaaatatctccggcctcatcttttgcccgcaggctaat- gtctgaaaattgaggtgacg ggttaaaaataatatccttggcaaccttttttatatcccttttaaattttggcttaatgactatatccaatgag- tcaaaaagctccccttcaatatct gttgcccctaagacctttaatatatcgccaaatacaggtagcttggcttctaccttcaccgttgttcggccgat- gaaatgcatatgcataacat cgtctttggtggttcccctcatcagtggctctatctgaacgcgctctccactgcttaatgacattcctttcccg- attaaaaaatctgtcagatcg gatgtggtcggcccgaaaacagttctggcaaaaccaatggtgtcgccttcaacaaacaaaaaagatgggaatcc- caatgattcgtcatct gcgaggctgttcttaatatcttcaactgaagctttagagcgatttatcttctgaaccagactcttgtcatttgt- tttggtaaagagaaaagtttttc catcgattttatgaatatacaaataattggagccaacctgcaggtgatgattatcagccagcagagaattaagg- aaaacagacaggtttatt gagcgcttatctttccctttatttttgctgcggtaagtcgcataaaaaccattcttcataattcaatccattta- ctatgttatgttctgaggggagt gaaaattcccctaattcgatgaagattcttgctcaattgttatcagctatgcgccgaccagaacaccttgccga- tcagccaaacgtctcttca ggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtt- tagtggttgtaaaaacac ctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctg- gctcaacagcctgctca gggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttc- aacctcaagccagaat gcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcagg- tgagaacatccctgcctgaa catgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtag- atttctctggcgattgaa gggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgat- gccattaaataaagcaccaa cgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttca- taaattgctttaaggcgacgt gcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatacgttaaatct

EQUIVALENTS

[0099] The present disclosure is not to be limited in terms of the particular embodiments described in this application. Many modifications and variations can be made without departing from its spirit and scope, as will be apparent to those skilled in the art. Functionally equivalent methods and apparatuses within the scope of the disclosure, in addition to those enumerated herein, will be apparent to those skilled in the art from the foregoing descriptions. Such modifications and variations are intended to fall within the scope of the appended claims. The present disclosure is to be limited only by the terms of the appended claims, along with the full scope of equivalents to which such claims are entitled. It is to be understood that this disclosure is not limited to particular methods, reagents, compounds compositions or biological systems, which can, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting.

[0100] In addition, where features or aspects of the disclosure are described in terms of Markush groups, those skilled in the art will recognize that the disclosure is also thereby described in terms of any individual member or subgroup of members of the Markush group.

[0101] As will be understood by one skilled in the art, for any and all purposes, particularly in terms of providing a written description, all ranges disclosed herein also encompass any and all possible subranges and combinations of subranges thereof. Any listed range can be easily recognized as sufficiently describing and enabling the same range being broken down into at least equal halves, thirds, quarters, fifths, tenths, etc. As a non-limiting example, each range discussed herein can be readily broken down into a lower third, middle third and upper third, etc. As will also be understood by one skilled in the art all language such as "up to," "at least," "greater than," "less than," and the like include the number recited and refer to ranges which can be subsequently broken down into subranges as discussed above. Finally, as will be understood by one skilled in the art, a range includes each individual member. Thus, for example, a group having 1-3 proteins refers to groups having 1, 2, or 3 proteins. Similarly, a group having 1-5 proteins refers to groups having 1, 2, 3, 4, or 5 proteins, and so forth.

[0102] While various aspects and embodiments have been disclosed herein, other aspects and embodiments will be apparent to those skilled in the art. The various aspects and embodiments disclosed herein are for purposes of illustration and are not intended to be limiting, with the true scope and spirit being indicated by the following claims.

[0103] All references cited herein are incorporated by reference herein in their entireties and for all purposes to the same extent as if each individual publication, patent, or patent application was specifically and individually incorporated by reference in its entirety for all purposes.

REFERENCES

[0104] Burbulis, I., Yamaguchi, K., Gordon, A., Carlson, R., Brent, R., 2005. Using protein-DNA chimeras to detect and count small numbers of molecules. Nat Methods 2, 31-37.

[0105] Chen, B. X., Szabolcs, M. J., Matsushima, A. Y., Erlanger, B. E. F., 1996. A strategy for immunohistochemical signal enhancement by end-product amplification. The journal of histochemistry and cytochemistry: official journal of the Histochemistry Society 44, 819-24.

[0106] Chiu, N. H., Christopoulos, T. K., 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin Chem 45, 1954-1959.

[0107] Christopoulos, T. K., Chiu, N. H., 1995. Expression immunoassay. Antigen quantitation using antibodies labeled with enzyme-coding DNA fragments. Anal Chem 67, 4290-4294.

[0108] Guo, Y., Zhou, Y.-F., Zhang, X., Zhang, Z., Qiao, Y., Bi, L.-J., Wen, J., Liang, M., Zhang, J., 2006. Phage display mediated immuno-PCR. Nucleic acids research 34, 4-9.

[0109] Hill, P. J., Rees, C. E., Winson, M. K., Stewart, G. S., 1993. The application of lux genes. Biotechnology and applied biochemistry 17 (Pt 1), 3-14.

[0110] Hill, P. J., Stewart, G. S., 1994. Use of lux genes in applied biochemistry. Journal of bioluminescence and chemiluminescence 9, 211-5.

[0111] Johannsson, A., Stanley, C. J., Self, C. H., 1985. A fast highly sensitive colorimetric enzyme immunoassay system demonstrating benefits of enzyme amplification in clinical chemistry. Clin Chim Acta 148, 119-124.

[0112] Kazane, S. A., Sok, D., Cho, E. H., Uson, M. L., Kuhn, P., Schultz, P. G., Smider, V. V, 2012. Site-specific DNA-antibody conjugates for specific and sensitive immuno-PCR. Proc Natl Acad Sci USA 109, 3731-3736.

[0113] Lansdorp, P. M., Van der Kwast, T. H., De Boer, M., Zeijlemaker, W. P., 1984. Stepwise amplified immunoperoxidase (PAP) staining I Cellular morphology in relation to membrane markers. J Histochem Cytochem 32, 172-178.

[0114] Lind, K., Kubista, M., 2005. Development and evaluation of three real-time immuno-PCR assemblages for quantification of PSA. J Immunol Methods 304, 107-116.

[0115] Manukhov, I. V, Rastorguev, S. M., Eroshnikov, G. E., Zarubina, A. P., Zavil'gel'ski{hacek over (i)}, G. B., 2000.

[0116] [Cloning and expression of the lux-operon of Photorhabdus luminescens, strain Zm1: nucleotide sequence of luxAB genes and basic properties of luciferase]. Genetika 36, 322-30.

[0117] Meighen, E. A., 1991. Molecular biology of bacterial bioluminescence. Microbiol Rev 55, 123-142.

[0118] Niemeyer, C. M., Adler, M., Wacker, R., 2007. Detecting antigens by quantitative immuno-PCR. Nat Protoc 2, 1918-1930.

[0119] Sano, T., Smith, C. L., Cantor, C. R., 1992. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science 258, 120-122.

[0120] Scholle, M. D., Kehoe, J. W., Kay, B. K., 2005. Efficient construction of a large collection of phage-displayed combinatorial peptide libraries. Comb Chem High Throughput Screen 8, 545-551.

[0121] Schweitzer, B., Wiltshire, S., Lambert, J., O'Malley, S., Kukanskis, K., Zhu, Z., Kingsmore, S. F., Lizardi, P. M., Ward, D. C., 2000. Inaugural article: immunoassays with rolling circle DNA amplification: a versatile platform for ultrasensitive antigen detection. Proc Natl Acad Sci USA 97, 10113-10119.

[0122] Smith, G. P., 1985. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228, 1315-1317.

[0123] Sternberger, L. A., Hardy Jr., P. H., Cuculis, J. J., Meyer, H. G., 1970. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18, 315-333.

[0124] Stewart, G. S., Williams, P., 1992. lux genes and the applications of bacterial bioluminescence. Journal of general microbiology 138, 1289-300.

[0125] Szittner, R., Meighen, E., 1990. Nucleotide sequence, expression, and properties of luciferase coded by lux genes from a terrestrial bacterium. The Journal of biological chemistry 265, 16581-7.

[0126] Takeda, S., Tsukiji, S., Nagamune, T., 2004. Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation. Bioorg Med Chem Lett 14, 2407-2410.

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Sequence CWU 1

1

34110990DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 1aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat 60atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact 120cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta 180gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca 240tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg 300ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag 360tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt 420cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca 480tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct 540aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt 600ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt 660aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg 720atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt 780tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca 840caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt 900ctcgtcaggg caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg 960aatatccggt tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc 1020tgtacaccgt tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc 1080gtctgcgcct cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat 1140caggcgatga tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt 1200caaagatgag tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta 1260gtggcattac gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct 1320caaagcctct gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga 1380cgatcccgca aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta 1440tgcgtgggcg atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa 1500attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 1560tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc 1620tattctcact ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca 1680tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt 1740ctgtggaatg ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca 1800tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 1860tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 1920attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 1980aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 2040cagaataata ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact 2100caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 2160tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa 2220gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 2280gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt 2340ggcggttctg agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt 2400gattttgatt atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 2460gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 2520gctgctatcg atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact 2580ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct 2640ttaatgaata atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct 2700tttgtcttta gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta 2760ttccgtggtg tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg 2820tttgctaaca tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt 2880tattattgcg tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc 2940ttaaaaaggg cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg 3000ggcttaactc aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact 3060ttgttcaggg tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc 3120tctctgtaaa ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg 3180attgggataa ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg 3240ctcgttagcg ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat 3300cttgatttaa ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt 3360cttagaatac cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat 3420tcctacgatg aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat 3480acccgttctt ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt 3540aaattaggat gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg 3600cgttctgcat tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct 3660tttgtcggta ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat 3720gttggcgttg ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat 3780actggtaaga atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat 3840tccggtgttt attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta 3900aatttaggtc agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt 3960tgtcttgcga ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg 4020gaggttaaaa aggtagtctc tcagacctat gattttgata aattcactat tgactcttct 4080cagcgtctta atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat 4140agcgacgatt tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc 4200attaaaaaag gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt 4260tgtttcatca tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt 4320tgtaacttgg tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg 4380tactgttact gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc 4440tgttttacgt gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta 4500taatccaaac aatcaggatt atattgatga attgccatca tctgataatc aggaatatga 4560tgataattcc gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac 4620ttttaaaatt aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa 4680gtctaatact tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt 4740tagtgcacct aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc 4800aactgaccag atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga 4860tttttcattt gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg 4920cctcacctct gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt 4980agggctatca gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg 5040tattcttacg ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat 5100tactggtcgt gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg 5160tcaaaatgta ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt 5220tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat 5280tactaatcaa agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact 5340cggtggcctc actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa 5400aatcccttta atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt 5460atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg 5520gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 5580tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 5640gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 5700atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 5760cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 5820ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa 5880acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg 5940ccaggcggtg aagggcaatc agctgttgcc cgtctcgctg gtgaaaagaa aaaccaccct 6000ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 6060acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 6120tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 6180ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcacta 6240gtgattatgg taaagcaaga tgaagttatc acattgttat caaatattcg tagtaatcga 6300tgcaagatat attctttgtt aggaagttcg catgacttga gtgaaaattt agtggtcctg 6360cgcaattttt atcaatcggt tacgaaagcc gctatcgcga tggataatga tcatctggat 6420attgatgttg atattactga accgtcattt gaacatttaa ctattgcgac agtcaatgaa 6480cgccgaatga gaattgagat tgaaaatcaa gcaatttctc tgtcttaaaa tctattgaga 6540tattctatca ctcaaatagc aatataagga ctctctatga aatttggaaa ctttttgctt 6600acataccaac ctccccaatt ttctcaaaca gaggtaatga aacgtttggt taaattaggt 6660cgcatctctg aggagtgtgg ttttgatacc gtatggttac tggagcatca tttcacggag 6720tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt tacttggcgc gactaaaaaa 6780ttgaatgtag gaactgccgc tattgttctt cccacagccc atccagtacg ccaacttgaa 6840gatgtgaatt tattggatca aatgtcaaaa ggacgatttc ggtttggtat ttgccgaggg 6900ctttacaaca aggactttcg cgtattcggc acagatatga ataacagtcg cgccttagcg 6960gaatgctggt acgggctgat aaagaatggc atgacagagg gatatatgga agctgataat 7020gaacatatca agttccataa ggtaaaagta aaccccgcgg cgtatagcag aggtggcgca 7080ccggtttatg tggtggctga atcagcttcg acgactgagt gggctgctca atttggccta 7140ccgatgatat taagttggat tataaatact aacgaaaaga aagcacaact tgagctttat 7200aatgaagtgg ctcaagaata tgggcacgat attcataata tcgaccattg cttatcatat 7260ataacatctg tagatcatga ctcaattaaa gcgaaagaga tttgccggaa atttctgggg 7320cattggtatg attcttatgt gaatgctacg actatttttg atgattcaga ccaaacaaga 7380ggttatgatt tcaataaagg gcagtggcgt gactttgtat taaaaggaca taaagatact 7440aatcgccgta ttgattacag ttacgaaatc aatcccgtgg gaacgccgca ggaatgtatt 7500gacataattc aaaaagacat tgatgctaca ggaatatcaa atatttgttg tggatttgaa 7560gctaatggaa cagtagacga aattattgct tccatgaagc tcttccagtc tgatgtcatg 7620ccatttctta aagaaaaaca acgttcgcta ttatattagc taaggagaaa gaaatgaaat 7680ttggattgtt cttccttaac ttcatcaatt caacaactgt tcaagaacaa agtatagttc 7740gcatgcagga aataacggag tatgttgata agttgaattt tgaacagatt ttagtgtatg 7800aaaatcattt ttcagataat ggtgttgtcg gcgctcctct gactgtttct ggttttctgc 7860tcggtttaac agagaaaatt aaaattggtt cattaaatca catcattaca actcatcatc 7920ctgtcgccat agcggaggaa gcttgcttat tggatcagtt aagtgaaggg agatttattt 7980tagggtttag tgattgcgaa aaaaaagatg aaatgcattt ttttaatcgc ccggttgaat 8040atcaacagca actatttgaa gagtgttatg aaatcattaa cgatgcttta acaacaggct 8100attgtaatcc agataacgat ttttatagct tccctaaaat atctgtaaat ccccatgctt 8160atacgccagg cggacctcgg aaatatgtaa cagcaaccag tcatcatatt gttgagtggg 8220cggccaaaaa aggtattcct ctcatcttta agtgggatga ttctaatgat gttagatatg 8280aatatgctga aagatataaa gccgttgcgg ataaatatga cgttgaccta tcagagatag 8340accatcagtt aatgatatta gttaactata acgaagatag taataaagct aaacaagaga 8400cgcgtgcatt tattagtgat tatgttcttg aaatgcaccc taatgaaaat ttcgaaaata 8460aacttgaaga aataattgca gaaaacgctg tcggaaatta tacggagtgt ataactgcgg 8520ctaagttggc aattgaaaag tgtggtgcga aaagtgtatt gctgtccttt gaaccaatga 8580atgatttgat gagccaaaaa aatgtaatca atattgttga tgataatatt aagaagtacc 8640acatggaata tacctaatag atttcgagtt gcagcgaggc ggcaagtgaa cgaatcccca 8700ggagcataga taactatgtg actggggtga gtgaaagcag ccaacaaagc agcagcttga 8760aagatgaagg gtataaaaga gtatgacagc agtgctgcca tactttctaa tattatcttg 8820aggagtaaaa caggtatgac ttcatatgtt gataaacaag aaattacagc aagctcagaa 8880attgatgatt tgattttttc gagcgatcca ttagtgtggt cttacgacga gcaggaaaaa 8940atcagaaaga aacttgtgct tgatgcattt cgtaatcatt ataaacattg tcgagaatat 9000cgtcactact gtcaggcaca caaagtagat gacaatatta cggaaattga tgacatacct 9060gtattcccaa catcggtttt taagtttact cgcttattaa cttctcagga aaacgagatt 9120gaaagttggt ttaccagtag cggcacgaat ggtttaaaaa gtcaggtggc gcgtgacaga 9180ttaagtattg agagactctt aggctctgtg agttatggca tgaaatatgt tggtagttgg 9240tttgatcatc aaatagaatt agtcaatttg ggaccagata gatttaatgc tcataatatt 9300tggtttaaat atgttatgag tttggtggaa ttgttatatc ctacgacatt taccgtaaca 9360gaagaacgaa tagattttgt taaaacattg aatagtcttg aacgaataaa aaatcaaggg 9420aaagatcttt gtcttattgg ttcgccatac tttatttatt tactctgcca ttatatgaaa 9480gataaaaaaa tctcattttc tggagataaa agcctttata tcataaccgg aggcggctgg 9540aaaagttacg aaaaagaatc tctgaaacgt gatgatttca atcatctttt atttgatact 9600ttcaatctca gtgatattag tcagatccga gatatattta atcaagttga actcaacact 9660tgtttctttg aggatgaaat gcagcgtaaa catgttccgc cgtgggtata tgcgcgagcg 9720cttgatcctg aaacgttgaa acctgtacct gatggaacgc cggggttgat gagttatatg 9780gatgcgtcag caaccagtta tccagcattt attgttaccg atgatgtcgg gataattagc 9840agagaatatg gtaagtatcc cggcgtgctc gttgaaattt tacgtcgcgt caatacgagg 9900acgcagaaag ggtgtgcttt aagcttaaac caagcattta atagttgaca taatcgaatt 9960cccgggagag ctcgatatcg catgcggtac ctctagaaga agcttgggat ccgtcgacct 10020gcagcaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 10080aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 10140gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt 10200ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata 10260cggtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg 10320taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt 10380actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt 10440ttgatggcgt tcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt 10500taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc tgtttttggg 10560gcttttctga ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt 10620catcgattct cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagatct 10680ctcaaaaata gctaccctct ccggcattaa tttatcagct agaacggttg aatatcatat 10740tgatggtgat ttgactgtct ccggcctttc tcaccctttt gaatctttac ctacacatta 10800ctcaggcatt gcatttaaaa tatatgaggg ttctaaaaat ttttatcctt gcgttgaaat 10860aaaggcttct cccgcaaaag tattacaggg tcataatgtt tttggtacaa ccgatttagc 10920tttatgctct gaggctttat tgcttaattt tgctaattct ttgccttgcc tgtatgattt 10980attggatgtt 10990238773DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 2tctcacagtg tacggaccta aagttccccc atagggggta cctaaagccc agccaatcac 60ctaaagtcaa ccttcggttg accttgaggg ttccctaagg gttggggatg acccttgggt 120ttgtctttgg gtgttacctt gagtgtctct ctgtgtccct atctgttaca gtctcctaaa 180gtatcctcct aaagtcacct cctaacgtcc atcctaaagc caacacctaa agcctacacc 240taaagaccca tcaagtcaac gcctatctta aagtttaaac ataaagacca gacctaaaga 300ccagacctaa agacactaca taaagaccag acctaaagac gccttgttgt tagccataaa 360gtgataacct ttaatcattg tctttattaa tacaactcac tataaggaga gacaacttaa 420agagacttaa aagattaatt taaaatttat caaaaagagt attgacttaa agtctaacct 480ataggatact tacagccatc gagagggaca cggcgaatag ccatcccaat cgacaccggg 540gtcaaccgga taagtagaca gcctgataag tcgcacgaca gaaagaaatt gaccgcgcta 600aggcccgtaa agaacgtcac gaggggcgct tagaggcacg cagattcaaa cgtcgcaacc 660gcaaggcacg taaagcacac aaagctaagc gcgaaagaat gcttgctgcg tggcgatggg 720ctgaacgtca agaacggcgt aaccatgagg tagctgtaga tgtactagga agaaccaata 780acgctatgct ctgggtcaac atgttctctg gggactttaa ggcgcttgag gaacgaatcg 840cgctgcactg gcgtaatgct gaccggatgg ctatcgctaa tggtcttacg ctcaacattg 900ataagcaact tgacgcaatg ttaatgggct gatagtctta tcttacaggt catctgcggg 960tggcctgaat aggtacgatt tactaactgg aagaggcact aaatgaacac gattaacatc 1020gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac 1080cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg 1140ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat 1200aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac 1260gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg 1320caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta 1380accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag 1440gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt 1500gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc 1560gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag 1620gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg 1680gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc 1740gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg 1800atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat 1860tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg 1920cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc 1980gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat 2040tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac 2100atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc 2160aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat 2220aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt 2280tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg 2340gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac 2400tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac 2460gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat 2520tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg 2580agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc 2640tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc 2700gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca 2760atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct 2820gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact 2880cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc 2940cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg 3000ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc 3060gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg 3120gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat 3180tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc 3240ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat 3300agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa 3360gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct 3420tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa 3480gcagtgcgcg aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac 3540gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct 3600aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaacgccaa 3660atcaatacga ctcactatag agggacaaac tcaaggtcat tcgcaagagt ggcctttatg 3720attgaccttc ttccggttaa tacgactcac tataggagaa ccttaaggtt taactttaag 3780acccttaagt gttaattaga gatttaaatt aaagaattac taagagagga ctttaagtat 3840gcgtaacttc gaaaagatga

ccaaacgttc taaccgtaat gctcgtgact tcgaggcaac 3900caaaggtcgc aagttgaata agactaagcg tgaccgctct cacaagcgta gctgggaggg 3960tcagtaagat gggacgttta tatagtggta atctggcagc attcaaggca gcaacaaaca 4020agctgttcca gttagactta gcggtcattt atgatgactg gtatgatgcc tatacaagaa 4080aagattgcat acggttacgt attgaggaca ggagtggaaa cctgattgat actagcacct 4140tctaccacca cgacgaggac gttctgttca atatgtgtac tgattggttg aaccatatgt 4200atgaccagtt gaaggactgg aagtaatacg actcagtata gggacaatgc ttaaggtcgc 4260tctctaggag tggccttagt catttaacca ataggagata aacattatga tgaacattaa 4320gactaacccg tttaaagccg tgtctttcgt agagtctgcc attaagaagg ctctggataa 4380cgctgggtat cttatcgctg aaatcaagta cgatggtgta cgcgggaaca tctgcgtaga 4440caatactgct aacagttact ggctctctcg tgtatctaaa acgattccgg cactggagca 4500cttaaacggg tttgatgttc gctggaagcg tctactgaac gatgaccgtt gcttctacaa 4560agatggcttt atgcttgatg gggaactcat ggtcaagggc gtagacttta acacagggtc 4620cggcctactg cgtaccaaat ggactgacac gaagaaccaa gagttccatg aagagttatt 4680cgttgaacca atccgtaaga aagataaagt tccctttaag ctgcacactg gacaccttca 4740cataaaactg tacgctatcc tcccgctgca catcgtggag tctggagaag actgtgatgt 4800catgacgttg ctcatgcagg aacacgttaa gaacatgctg cctctgctac aggaatactt 4860ccctgaaatc gaatggcaag cggctgaatc ttacgaggtc tacgatatgg tagaactaca 4920gcaactgtac gagcagaagc gagcagaagg ccatgagggt ctcattgtga aagacccgat 4980gtgtatctat aagcgcggta agaaatctgg ctggtggaaa atgaaacctg agaacgaagc 5040tgacggtatc attcagggtc tggtatgggg tacaaaaggt ctggctaatg aaggtaaagt 5100gattggtttt gaggtgcttc ttgagagtgg tcgtttagtt aacgccacga atatctctcg 5160cgccttaatg gatgagttca ctgagacagt aaaagaggcc accctaagtc aatggggatt 5220ctttagccca tacggtattg gcgacaacga tgcttgtact attaaccctt acgatggctg 5280ggcgtgtcaa attagctaca tggaggaaac acctgatggc tctttgcggc acccatcgtt 5340cgtaatgttc cgtggcaccg aggacaaccc tcaagagaaa atgtaatcac actggctcac 5400cttcgggtgg gcctttctgc gtttataagg agacacttta tgtttaagaa ggttggtaaa 5460ttccttgcgg ctttggcagc tatcctgacg cttgcgtata ttcttgcggt ataccctcaa 5520gtagcactag tagtagttgg cgcttgttac ttagcggcag tgtgtgcttg cgtgtggagt 5580atagttaact ggtaatacga ctcactaaag gaggtacaca ccatgatgta cttaatgcca 5640ttactcatcg tcattgtagg atgccttgcg ctccactgta gcgatgatga tatgccagat 5700ggtcacgctt aatacgactc actaaaggag acactatatg tttcgacttc attacaacaa 5760aagcgttaag aatttcacgg ttcgccgtgc tgaccgttca atcgtatgtg cgagcgagcg 5820ccgagctaag atacctctta ttggtaacac agttcctttg gcaccgagcg tccacatcat 5880tatcacccgt ggtgactttg agaaagcaat agacaagaaa cgtccggttc ttagtgtggc 5940agtgacccgc ttcccgttcg tccgtctgtt actcaaacga atcaaggagg tgttctgatg 6000ggactgttag atggtgaagc ctgggaaaaa gaaaacccgc cagtacaagc aactgggtgt 6060atagcttgct tagagaaaga tgaccgttat ccacacacct gtaacaaagg agctaacgat 6120atgaccgaac gtgaacaaga gatgatcatt aagttgatag acaataatga aggtcgccca 6180gatgatttga atggctgcgg tattctctgc tccaatgtcc cttgccacct ctgccccgca 6240aataacgatc aaaagataac cttaggtgaa atccgagcga tggacccacg taaaccacat 6300ctgaataaac ctgaggtaac tcctacagat gaccagcctt ccgctgagac aatcgaaggt 6360gtcactaagc cttcccacta catgctgttt gacgacattg aggctatcga agtgattgct 6420cgttcaatga ccgttgagca gttcaaggga tactgcttcg gtaacatctt aaagtacaga 6480ctacgtgctg gtaagaagtc agagttagcg tacttagaga aagacctagc gaaagcagac 6540ttctataaag aactctttga gaaacataag gataaatgtt atgcataact tcaagtcaac 6600cccacctgcc gacagcctat ctgatgactt cacatcttgc tcagagtggt gccgaaagat 6660gtgggaagag acattcgacg atgcgtacat caagctgtat gaactttgga aatcgagagg 6720tcaatgacta tgtcaaacgt aaatacaggt tcacttagtg tggacaataa gaagttttgg 6780gctaccgtag agtcctcgga gcattccttc gaggttccaa tctacgctga gaccctagac 6840gaagctctgg agttagccga atggcaatac gttccggctg gctttgaggt tactcgtgtg 6900cgtccttgtg tagcaccgaa gtaatacgac tcactattag ggaagactcc ctctgagaaa 6960ccaaacgaaa cctaaaggag attaacatta tggctaagaa gattttcacc tctgcgctgg 7020gtaccgctga accttacgct tacatcgcca agccggacta cggcaacgaa gagcgtggct 7080ttgggaaccc tcgtggtgtc tataaagttg acctgactat tcccaacaaa gacccgcgct 7140gccagcgtat ggtcgatgaa atcgtgaagt gtcacgaaga ggcttatgct gctgccgttg 7200aggaatacga agctaatcca cctgctgtag ctcgtggtaa gaaaccgctg aaaccgtatg 7260agggtgacat gccgttcttc gataacggtg acggtacgac tacctttaag ttcaaatgct 7320acgcgtcttt ccaagacaag aagaccaaag agaccaagca catcaatctg gttgtggttg 7380actcaaaagg taagaagatg gaagacgttc cgattatcgg tggtggctct aagctgaaag 7440ttaaatattc tctggttcca tacaagtgga acactgctgt aggtgcgagc gttaagctgc 7500aactggaatc cgtgatgctg gtcgaactgg ctacctttgg tggcggtgaa gacgattggg 7560ctgacgaagt tgaagagaac ggctatgttg cctctggttc tgccaaagcg agcaaaccac 7620gcgacgaaga aagctgggac gaagacgacg aagagtccga ggaagcagac gaagacggag 7680acttctaagt ggaactgcgg gagaaaatcc ttgagcgaat caaggtgact tcctctgggt 7740gttgggagtg gcagggcgct acgaacaata aagggtacgg gcaggtgtgg tgcagcaata 7800ccggaaaggt tgtctactgt catcgcgtaa tgtctaatgc tccgaaaggt tctaccgtcc 7860tgcactcctg tgataatcca ttatgttgta accctgaaca cctatccata ggaactccaa 7920aagagaactc cactgacatg gtaaataagg gtcgctcaca caaggggtat aaactttcag 7980acgaagacgt aatggcaatc atggagtcca gcgagtccaa tgtatcctta gctcgcacct 8040atggtgtctc ccaacagact atttgtgata tacgcaaagg gaggcgacat ggcaggttac 8100ggcgctaaag gaatccgaaa ggttggagcg tttcgctctg gcctagagga caaggtttca 8160aagcagttgg aatcaaaagg tattaaattc gagtatgaag agtggaaagt gccttatgta 8220attccggcga gcaatcacac ttacactcca gacttcttac ttccaaacgg tatattcgtt 8280gagacaaagg gtctgtggga aagcgatgat agaaagaagc acttattaat tagggagcag 8340caccccgagc tagacatccg tattgtcttc tcaagctcac gtactaagtt atacaaaggt 8400tctccaacgt cttatggaga gttctgcgaa aagcatggta ttaagttcgc tgataaactg 8460atacctgctg agtggataaa ggaacccaag aaggaggtcc cctttgatag attaaaaagg 8520aaaggaggaa agaaataatg gctcgtgtac agtttaaaca acgtgaatct actgacgcaa 8580tctttgttca ctgctcggct accaagccaa gtcagaatgt tggtgtccgt gagattcgcc 8640agtggcacaa agagcagggt tggctcgatg tgggatacca ctttatcatc aagcgagacg 8700gtactgtgga ggcaggacga gatgagatgg ctgtaggctc tcacgctaag ggttacaacc 8760acaactctat cggcgtctgc cttgttggtg gtatcgacga taaaggtaag ttcgacgcta 8820actttacgcc agcccaaatg caatcccttc gctcactgct tgtcacactg ctggctaagt 8880acgaaggcgc tggtcttcgc gcccatcatg aggtggcgcc gaaggcttgc ccttcgttcg 8940accttaagcg ttggtgggag aagaacgaac tggtcacttc tgaccgtgga taatgatcta 9000ttggaagtcg ttgcgtggat ttatagaact aggagggaat tgcatggaca attcgcacga 9060ttccgatagt gtatttcttt accacattcc ttgtgacaac tgtgggagta gtgatgggaa 9120ctcgctgttc tctgacggac acacgttctg ctacgtatgc gagaagtgga ctgctggtaa 9180tgaagacact aaagagaggg cttcaaaacg gaaaccctca ggaggtaaac caatgactta 9240caacgtgtgg aacttcgggg aatccaatgg acgctactcc gcgttaactg cgagaggaat 9300ctccaaggaa acctgtcaga aggctggcta ctggattgcc aaagtagacg gtgtgatgta 9360ccaagtggct gactatcggg accagaacgg caacattgtg agtcagaagg ttcgagataa 9420agataagaac tttaagacca ctggtagtca caagagtgac gctctgttcg ggaagcactt 9480gtggaatggt ggtaagaaga ttgtcgttac agaaggtgaa atcgacatgc ttaccgtgat 9540ggaacttcaa gactgtaagt atcctgtagt gtcgttgggt cacggtgcct ctgccgctaa 9600gaagacatgc gctgccaact acgaatactt tgaccagttc gaacagatta tcttaatgtt 9660cgatatggac gaagcagggc gcaaagcagt cgaagaggct gcacaggttc tacctgctgg 9720taaggtacga gtggcagttc ttccgtgtaa ggatgcaaac gagtgtcacc taaatggtca 9780cgaccgtgaa atcatggagc aagtgtggaa tgctggtcct tggattcctg atggtgtggt 9840atcggctctt tcgttacgtg aacgaatccg tgagcaccta tcgtccgagg aatcagtagg 9900tttacttttc agtggctgca ctggtatcaa cgataagacc ttaggtgccc gtggtggtga 9960agtcattatg gtcacttccg gttccggtat gggtaagtca acgttcgtcc gtcaacaagc 10020tctacaatgg ggcacagcga tgggcaagaa ggtaggctta gcgatgcttg aggagtccgt 10080tgaggagacc gctgaggacc ttataggtct acacaaccgt gtccgactga gacaatccga 10140ctcactaaag agagagatta ttgagaacgg taagttcgac caatggttcg atgaactgtt 10200cggcaacgat acgttccatc tatatgactc attcgccgag gctgagacgg atagactgct 10260cgctaagctg gcctacatgc gctcaggctt gggctgtgac gtaatcattc tagaccacat 10320ctcaatcgtc gtatccgctt ctggtgaatc cgatgagcgt aagatgattg acaacctgat 10380gaccaagctc aaagggttcg ctaagtcaac tggggtggtg ctggtcgtaa tttgtcacct 10440taagaaccca gacaaaggta aagcacatga ggaaggtcgc cccgtttcta ttactgacct 10500acgtggttct ggcgcactac gccaactatc tgatactatt attgcccttg agcgtaatca 10560gcaaggcgat atgcctaacc ttgtcctcgt tcgtattctc aagtgccgct ttactggtga 10620tactggtatc gctggctaca tggaatacaa caaggaaacc ggatggcttg aaccatcaag 10680ttactcaggg gaagaagagt cacactcaga gtcaacagac tggtccaacg acactgactt 10740ctgacaggat tcttgacagt tgtttcatat gaagagattg ttaagtcacg ataatcaata 10800ggagaaatca atatgatcgt ttctgacatc gaagctaacg ccctcttaga gagcgtcact 10860aagttccact gcggggttat ctacgactac tccaccgctg agtacgtaag ctaccgtccg 10920agtgacttcg gtgcgtatct ggatgcgctg gaagccgagg ttgcacgagg cggtcttatt 10980gtgttccaca acggtcacaa gtatgacgtt cctgcattga ccaaactggc aaagttgcaa 11040ttgaaccgag agttccacct tcctcgtgag aactgtattg acacccttgt gttgtcacgt 11100ttgattcatt ccaacctcaa ggacaccgat atgggtcttc tgcgttccgg caagttgccc 11160ggaaaacgct ttgggtctca cgctttggag gcgtggggtt atcgcttagg cgagatgaag 11220ggtgaataca aagacgactt taagcgtatg cttgaagagc agggtgaaga atacgttgac 11280ggaatggagt ggtggaactt caacgaagag atgatggact ataacgttca ggacgttgtg 11340gtaactaaag ctctccttga gaagctactc tctgacaaac attacttccc tcctgagatt 11400gactttacgg acgtaggata cactacgttc tggtcagaat cccttgaggc cgttgacatt 11460gaacatcgtg ctgcatggct gctcgctaaa caagagcgca acgggttccc gtttgacaca 11520aaagcaatcg aagagttgta cgtagagtta gctgctcgcc gctctgagtt gctccgtaaa 11580ttgaccgaaa cgttcggctc gtggtatcag cctaaaggtg gcactgagat gttctgccat 11640ccgcgaacag gtaagccact acctaaatac cctcgcatta agacacctaa agttggtggt 11700atctttaaga agcctaagaa caaggcacag cgagaaggcc gtgagccttg cgaacttgat 11760acccgcgagt acgttgctgg tgctccttac accccagttg aacatgttgt gtttaaccct 11820tcgtctcgtg accacattca gaagaaactc caagaggctg ggtgggtccc gaccaagtac 11880accgataagg gtgctcctgt ggtggacgat gaggtactcg aaggagtacg tgtagatgac 11940cctgagaagc aagccgctat cgacctcatt aaagagtact tgatgattca gaagcgaatc 12000ggacagtctg ctgagggaga caaagcatgg cttcgttatg ttgctgagga tggtaagatt 12060catggttctg ttaaccctaa tggagcagtt acgggtcgtg cgacccatgc gttcccaaac 12120cttgcgcaaa ttccgggtgt acgttctcct tatggagagc agtgtcgcgc tgcttttggc 12180gctgagcacc atttggatgg gataactggt aagccttggg ttcaggctgg catcgacgca 12240tccggtcttg agctacgctg cttggctcac ttcatggctc gctttgataa cggcgagtac 12300gctcacgaga ttcttaacgg cgacatccac actaagaacc agatagctgc tgaactacct 12360acccgagata acgctaagac gttcatctat gggttcctct atggtgctgg tgatgagaag 12420attggacaga ttgttggtgc tggtaaagag cgcggtaagg aactcaagaa gaaattcctt 12480gagaacaccc ccgcgattgc agcactccgc gagtctatcc aacagacact tgtcgagtcc 12540tctcaatggg tagctggtga gcaacaagtc aagtggaaac gccgctggat taaaggtctg 12600gatggtcgta aggtacacgt tcgtagtcct cacgctgcct tgaataccct actgcaatct 12660gctggtgctc tcatctgcaa actgtggatt atcaagaccg aagagatgct cgtagagaaa 12720ggcttgaagc atggctggga tggggacttt gcgtacatgg catgggtaca tgatgaaatc 12780caagtaggct gccgtaccga agagattgct caggtggtca ttgagaccgc acaagaagcg 12840atgcgctggg ttggagacca ctggaacttc cggtgtcttc tggataccga aggtaagatg 12900ggtcctaatt gggcgatttg ccactgatac aggaggctac tcatgaacga aagacactta 12960acaggtgctg cttctgaaat gctagtagcc tacaaattta ccaaagctgg gtacactgtc 13020tattacccta tgctgactca gagtaaagag gacttggttg tatgtaagga tggtaaattt 13080agtaaggttc aggttaaaac agccacaacg gttcaaacca acacaggaga tgccaagcag 13140gttaggctag gtggatgcgg taggtccgaa tataaggatg gagactttga cattcttgcg 13200gttgtggttg acgaagatgt gcttattttc acatgggacg aagtaaaagg taagacatcc 13260atgtgtgtcg gcaagagaaa caaaggcata aaactatagg agaaattatt atggctatga 13320caaagaaatt taaagtgtcc ttcgacgtta ccgcaaagat gtcgtctgac gttcaggcaa 13380tcttagagaa agatatgctg catctatgta agcaggtcgg ctcaggtgcg attgtcccca 13440atggtaaaca gaaggaaatg attgtccagt tcctgacaca cggtatggaa ggattgatga 13500cattcgtagt acgtacatca tttcgtgagg ccattaagga catgcacgaa gagtatgcag 13560ataaggactc tttcaaacaa tctcctgcaa cagtacggga ggtgttctga tgtctgacta 13620cctgaaagtg ctgcaagcaa tcaaaagttg ccctaagact ttccagtcca actatgtacg 13680gaacaatgcg agcctcgtag cggaggccgc ttcccgtggt cacatctcgt gcctgactac 13740tagtggacgt aacggtggcg cttgggaaat cactgcttcc ggtactcgct ttctgaaacg 13800aatgggagga tgtgtctaat gtctcgtgac cttgtgacta ttccacgcga tgtgtggaac 13860gatatacagg gctacatcga ctctctggaa cgtgagaacg atagccttaa gaatcaacta 13920atggaagctg acgaatacgt agcggaacta gaggagaaac ttaatggcac ttcttgacct 13980taaacaattc tatgagttac gtgaaggctg cgacgacaag ggtatccttg tgatggacgg 14040cgactggctg gtcttccaag ctatgagtgc tgctgagttt gatgcctctt gggaggaaga 14100gatttggcac cgatgctgtg accacgctaa ggcccgtcag attcttgagg attccattaa 14160gtcctacgag acccgtaaga aggcttgggc aggtgctcca attgtccttg cgttcaccga 14220tagtgttaac tggcgtaaag aactggttga cccgaactat aaggctaacc gtaaggccgt 14280gaagaaacct gtagggtact ttgagttcct tgatgctctc tttgagcgcg aagagttcta 14340ttgcatccgt gagcctatgc ttgagggtga tgacgttatg ggagttattg cttccaatcc 14400gtctgccttc ggtgctcgta aggctgtaat catctcttgc gataaggact ttaagaccat 14460ccctaactgt gacttcctgt ggtgtaccac tggtaacatc ctgactcaga ccgaagagtc 14520cgctgactgg tggcacctct tccagaccat caagggtgac atcactgatg gttactcagg 14580gattgctgga tggggtgata ccgccgagga cttcttgaat aacccgttca taaccgagcc 14640taaaacgtct gtgcttaagt ccggtaagaa caaaggccaa gaggttacta aatgggttaa 14700acgcgaccct gagcctcatg agacgctttg ggactgcatt aagtccattg gcgcgaaggc 14760tggtatgacc gaagaggata ttatcaagca gggccaaatg gctcgaatcc tacggttcaa 14820cgagtacaac tttattgaca aggagattta cctgtggaga ccgtagcgta tattggtctg 14880ggtctttgtg ttctcggagt gtgcctcatt tcgtggggcc tttgggactt agccagaata 14940atcaagtcgt tacacgacac taagtgataa actcaaggtc cctaaattaa tacgactcac 15000tatagggaga taggggcctt tacgattatt actttaagat ttaactctaa gaggaatctt 15060tattatgtta acacctatta accaattact taagaaccct aacgatattc cagatgtacc 15120tcgtgcaacc gctgagtatc tacaggttcg attcaactat gcgtacctcg aagcgtctgg 15180tcatatagga cttatgcgtg ctaatggttg tagtgaggcc cacatcttgg gtttcattca 15240gggcctacag tatgcctcta acgtcattga cgagattgag ttacgcaagg aacaactaag 15300agatgatggg gaggattgac actatgtgtt tctcaccgaa aattaaaact ccgaagatgg 15360ataccaatca gattcgagcc gttgagccag cgcctctgac ccaagaagtg tcaagcgtgg 15420agttcggtgg gtcttctgat gagacggata ccgagggcac cgaagtgtct ggacgcaaag 15480gcctcaaggt cgaacgtgat gattccgtag cgaagtctaa agccagcggc aatggctccg 15540ctcgtatgaa atcttccatc cgtaagtccg catttggagg taagaagtga tgtctgagtt 15600cacatgtgtg gaggctaaga gtcgcttccg tgcaatccgg tggactgtgg aacaccttgg 15660gttgcctaaa ggattcgaag gacactttgt gggctacagc ctctacgtag acgaagtgat 15720ggacatgtct ggttgccgtg aagagtacat tctggactct accggaaaac atgtagcgta 15780cttcgcgtgg tgcgtaagct gtgacattca ccacaaagga gacattctgg atgtaacgtc 15840cgttgtcatt aatcctgagg cagactctaa gggcttacag cgattcctag cgaaacgctt 15900taagtacctt gcggaactcc acgattgcga ttgggtgtct cgttgtaagc atgaaggcga 15960gacaatgcgt gtatacttta aggaggtata agttatgggt aagaaagtta agaaggccgt 16020gaagaaagtc accaagtccg ttaagaaagt cgttaaggaa ggggctcgtc cggttaaaca 16080ggttgctggc ggtctagctg gtctggctgg tggtactggt gaagcacaga tggtggaagt 16140accacaagct gccgcacaga ttgttgacgt acctgagaaa gaggtttcca ctgaggacga 16200agcacagaca gaaagcggac gcaagaaagc tcgtgctggc ggtaagaaat ccttgagtgt 16260agcccgtagc tccggtggcg gtatcaacat ttaatcagga ggttatcgtg gaagactgca 16320ttgaatggac cggaggtgtc aactctaagg gttatggtcg taagtgggtt aatggtaaac 16380ttgtgactcc acataggcac atctatgagg agacatatgg tccagttcca acaggaattg 16440tggtgatgca tatctgcgat aaccctaggt gctataacat aaagcacctt acgcttggaa 16500ctccaaagga taattccgag gacatggtta ccaaaggtag acaggctaaa ggagaggaac 16560taagcaagaa acttacagag tcagacgttc tcgctatacg ctcttcaacc ttaagccacc 16620gctccttagg agaactgtat ggagtcagtc aatcaaccat aacgcgaata ctacagcgta 16680agacatggag acacatttaa tggctgagaa acgaacagga cttgcggagg atggcgcaaa 16740gtctgtctat gagcgtttaa agaacgaccg tgctccctat gagacacgcg ctcagaattg 16800cgctcaatat accatcccat cattgttccc taaggactcc gataacgcct ctacagatta 16860tcaaactccg tggcaagccg tgggcgctcg tggtctgaac aatctagcct ctaagctcat 16920gctggctcta ttccctatgc agacttggat gcgacttact atatctgaat atgaagcaaa 16980gcagttactg agcgaccccg atggactcgc taaggtcgat gagggcctct cgatggtaga 17040gcgtatcatc atgaactaca ttgagtctaa cagttaccgc gtgactctct ttgaggctct 17100caaacagtta gtcgtagctg gtaacgtcct gctgtaccta ccggaaccgg aagggtcaaa 17160ctataatccc atgaagctgt accgattgtc ttcttatgtg gtccaacgag acgcattcgg 17220caacgttctg caaatggtga ctcgtgacca gatagctttt ggtgctctcc ctgaggacat 17280ccgtaaggct gtagaaggtc aaggtggtga gaagaaagct gatgagacaa tcgacgtgta 17340cactcacatc tatctggatg aggactcagg tgaatacctc cgatacgaag aggtcgaggg 17400tatggaagtc caaggctccg atgggactta tcctaaagag gcttgcccat acatcccgat 17460tcggatggtc agactagatg gtgaatccta cggtcgttcg tacattgagg aatacttagg 17520tgacttacgg tcccttgaaa atctccaaga ggctatcgtc aagatgtcca tgattagctc 17580taaggttatc ggcttagtga atcctgctgg tatcacccag ccacgccgac tgaccaaagc 17640tcagactggt gacttcgtta ctggtcgtcc agaagacatc tcgttcctcc aactggagaa 17700gcaagcagac tttactgtag ctaaagccgt aagtgacgct atcgaggctc gcctttcgtt 17760tgcctttatg ttgaactctg cggttcagcg tacaggtgaa cgtgtgaccg ccgaagagat 17820tcggtatgta gcttctgaac ttgaagatac tttaggtggt gtctactcta tcctttctca 17880agaattacaa ttgcctctgg tacgagtgct cttgaagcaa ctacaagcca cgcaacagat 17940tcctgagtta cctaaggaag ccgtagagcc aaccattagt acaggtctgg aagcaattgg 18000tcgaggacaa gaccttgata agctggagcg gtgtgtcact gcgtgggctg cactggcacc 18060tatgcgggac gaccctgata ttaaccttgc gatgattaag ttacgtattg ccaacgctat 18120cggtattgac acttctggta ttctactcac cgaagaacag aagcaacaga agatggccca 18180acagtctatg caaatgggta tggataatgg tgctgctgcg ctggctcaag gtatggctgc 18240acaagctaca gcttcacctg aggctatggc tgctgccgct gattccgtag gtttacagcc 18300gggaatttaa tacgactcac tatagggaga cctcatcttt gaaatgagcg atgacaagag 18360gttggagtcc tcggtcttcc tgtagttcaa ctttaaggag acaataataa tggctgaatc 18420taatgcagac gtatatgcat cttttggcgt gaactccgct gtgatgtctg gtggttccgt 18480tgaggaacat gagcagaaca tgctggctct tgatgttgct gcccgtgatg gcgatgatgc 18540aatcgagtta gcgtcagacg aagtggaaac agaacgtgac ctgtatgaca actctgaccc 18600gttcggtcaa gaggatgacg aaggccgcat tcaggttcgt atcggtgatg gctctgagcc 18660gaccgatgtg gacactggag aagaaggcgt tgagggcacc gaaggttccg aagagtttac 18720cccactgggc gagactccag aagaactggt agctgcctct gagcaacttg gtgagcacga 18780agagggcttc caagagatga ttaacattgc tgctgagcgt ggcatgagtg tcgagaccat 18840tgaggctatc cagcgtgagt acgaggagaa cgaagagttg tccgccgagt cctacgctaa 18900gctggctgaa attggctaca

cgaaggcttt cattgactcg tatatccgtg gtcaagaagc 18960tctggtggag cagtacgtaa acagtgtcat tgagtacgct ggtggtcgtg aacgttttga 19020tgcactgtat aaccaccttg agacgcacaa ccctgaggct gcacagtcgc tggataatgc 19080gttgaccaat cgtgacttag cgaccgttaa ggctatcatc aacttggctg gtgagtctcg 19140cgctaaggcg ttcggtcgta agccaactcg tagtgtgact aatcgtgcta ttccggctaa 19200acctcaggct accaagcgtg aaggctttgc ggaccgtagc gagatgatta aagctatgag 19260tgaccctcgg tatcgcacag atgccaacta tcgtcgtcaa gtcgaacaga aagtaatcga 19320ttcgaacttc taactagatc tcattatcat atggctagca tgactggtgg acagcaaatg 19380ggtactaacc aaggtaaagg tgtagttgct gctggagata aactggcgtt gttcttgaag 19440gtatttggcg gtgaagtcct gactgcgttc gctcgtacct ccgtgaccac ttctcgccac 19500atggtacgtt ccatctccag cggtaaatcc gctcagttcc ctgttctggg tcgcactcag 19560gcagcgtatc tggctccggg cgagaacctc gacgataaac gtaaggacat caaacacacc 19620gagaaggtaa tcaccattga cggtctcctg acggctgacg ttctgattta tgatattgag 19680gacgcgatga accactacga cgttcgctct gagtatacct ctcagttggg tgaatctctg 19740gcgatggctg cggatggtgc ggttctggct gagattgccg gtctgtgtaa cgtggaaagc 19800aaatataatg agaacatcga gggcttaggt actgctaccg taattgagac cactcagaac 19860aaggccgcac ttaccgacca agttgcgctg ggtaaggaga ttattgcggc tctgactaag 19920gctcgtgcgg ctctgaccaa gaactatgtt ccggctgctg accgtgtgtt ctactgtgac 19980ccagatagct actctgcgat tctggcagca ctgatgccga acgcagcaaa ctacgctgct 20040ctgattgacc ctgagaaggg ttctatccgc aacgttatgg gctttgaggt tgtagaagtt 20100ccgcacctca ccgctggtgg tgctggtacc gctcgtgagg gcactactgg tcagaagcac 20160gtcttccctg ccaataaagg tgagggtaat gtcaaggttg ctaaggacaa cgttatcggc 20220ctgttcatgc accgctctgc ggtaggtact gttaagctgc gtgacttggc tctggagcgc 20280gctcgccgtg ctaacttcca agcggaccag attatcgcta agtacgcaat gggccacggt 20340ggtcttcgcc cagaagctgc aggagctgtc gtattccagt caggtgtgat gctcggggat 20400ctcgaggggg cccaggcggc cgcactcgac tcggtacccc gatggataat gatcatctgg 20460atattgatgt tgatattact gaaccgtcat ttgaacattt aactattgcg acagtcaatg 20520aacgccgaat gagaattgag attgaaaatc aagcaatttc tctgtcttaa aatctattga 20580gatattctat cactcaaata gcaatataag gactctctat gaaatttgga aactttttgc 20640ttacatacca acctccccaa ttttctcaaa cagaggtaat gaaacgtttg gttaaattag 20700gtcgcatctc tgaggagtgt ggttttgata ccgtatggtt actggagcat catttcacgg 20760agtttggttt gcttggtaac ccttatgtcg ctgctgcata tttacttggc gcgactaaaa 20820aattgaatgt aggaactgcc gctattgttc ttcccacagc ccatccagta cgccaacttg 20880aagatgtgaa tttattggat caaatgtcaa aaggacgatt tcggtttggt atttgccgag 20940ggctttacaa caaggacttt cgcgtattcg gcacagatat gaataacagt cgcgccttag 21000cggaatgctg gtacgggctg ataaagaatg gcatgacaga gggatatatg gaagctgata 21060atgaacatat caagttccat aaggtaaaag taaaccccgc ggcgtatagc agaggtggcg 21120caccggttta tgtggtggct gaatcagctt cgacgactga gtgggctgct caatttggcc 21180taccgatgat attaagttgg attataaata ctaacgaaaa gaaagcacaa cttgagcttt 21240ataatgaagt ggctcaagaa tatgggcacg atattcataa tatcgaccat tgcttatcat 21300atataacatc tgtagatcat gactcaatta aagcgaaaga gatttgccgg aaatttctgg 21360ggcattggta tgattcttat gtgaatgcta cgactatttt tgatgattca gaccaaacaa 21420gaggttatga tttcaataaa gggcagtggc gtgactttgt attaaaagga cataaagata 21480ctaatcgccg tattgattac agttacgaaa tcaatcccgt gggaacgccg caggaatgta 21540ttgacataat tcaaaaagac attgatgcta caggaatatc aaatatttgt tgtggatttg 21600aagctaatgg aacagtagac gaaattattg cttccatgaa gctcttccag tctgatgtca 21660tgccatttct taaagaaaaa caacgttcgc tattatatta gctaaggaga aagaaatgaa 21720atttggattg ttcttcctta acttcatcaa ttcaacaact gttcaagaac aaagtatagt 21780tcgcatgcag gaaataacgg agtatgttga taagttgaat tttgaacaga ttttagtgta 21840tgaaaatcat ttttcagata atggtgttgt cggcgctcct ctgactgttt ctggttttct 21900gctcggttta acagagaaaa ttaaaattgg ttcattaaat cacatcatta caactcatca 21960tcctgtcgcc atagcggagg aagcttgctt attggatcag ttaagtgaag ggagatttat 22020tttagggttt agtgattgcg aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga 22080atatcaacag caactatttg aagagtgtta tgaaatcatt aacgatgctt taacaacagg 22140ctattgtaat ccagataacg atttttatag cttccctaaa atatctgtaa atccccatgc 22200ttatacgcca ggcggacctc ggaaatatgt aacagcaacc agtcatcata ttgttgagtg 22260ggcggccaaa aaaggtattc ctctcatctt taagtgggat gattctaatg atgttagata 22320tgaatatgct gaaagatata aagccgttgc ggataaatat gacgttgacc tatcagagat 22380agaccatcag ttaatgatat tagttaacta taacgaagat agtaataaag ctaaacaaga 22440gacgcgtgca tttattagtg attatgttct tgaaatgcac cctaatgaaa atttcgaaaa 22500taaacttgaa gaaataattg cagaaaacgc tgtcggaaat tatacggagt gtataactgc 22560ggctaagttg gcaattgaaa agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat 22620gaatgatttg atgagccaaa aaaatgtaat caatattgtt gatgataata ttaagaagta 22680ccacatggaa tatacctaat agatttcgag ttgcagcgag gcggcaagtg aacgaatccc 22740caggagcata gataactatg tgactggggt gagtgaaagc agccaacaaa gcagcagctt 22800gaaagatgaa gggtataaaa gagtatgaca gcagtgctgc catactttct aatattatct 22860tgaggagtaa aacaggtatg acttcatatg ttgataaaca agaaattaca gcaagctcag 22920aaattgatga tttgattttt tcgagcgatc catctcgaat tcgagctccg tcgacaagct 22980tgcggccgca ctcgagtaac tagttaaccc cttggggcct ctaaacgggt cttgaggggt 23040tttttgctga aaggaggaac tatatgcgct catacgatat gaacgttgag actgccgctg 23100agttatcagc tgtgaacgac attctggcgt ctatcggtga acctccggta tcaacgctgg 23160aaggtgacgc taacgcagat gcagcgaacg ctcggcgtat tctcaacaag attaaccgac 23220agattcaatc tcgtggatgg acgttcaaca ttgaggaagg cataacgcta ctacctgatg 23280tttactccaa cctgattgta tacagtgacg actatttatc cctaatgtct acttccggtc 23340aatccatcta cgttaaccga ggtggctatg tgtatgaccg aacgagtcaa tcagaccgct 23400ttgactctgg tattactgtg aacattattc gtctccgcga ctacgatgag atgcctgagt 23460gcttccgtta ctggattgtc accaaggctt cccgtcagtt caacaaccga ttctttgggg 23520caccggaagt agagggtgta ctccaagaag aggaagatga ggctagacgt ctctgcatgg 23580agtatgagat ggactacggt gggtacaata tgctggatgg agatgcgttc acttctggtc 23640tactgactcg ctaacattaa taaataagga ggctctaatg gcactcatta gccaatcaat 23700caagaacttg aagggtggta tcagccaaca gcctgacatc cttcgttatc cagaccaagg 23760gtcacgccaa gttaacggtt ggtcttcgga gaccgagggc ctccaaaagc gtccacctct 23820tgttttctta aatacacttg gagacaacgg tgcgttaggt caagctccgt acatccacct 23880gattaaccga gatgagcacg aacagtatta cgctgtgttc actggtagcg gaatccgagt 23940gttcgacctt tctggtaacg agaagcaagt taggtatcct aacggttcca actacatcaa 24000gaccgctaat ccacgtaacg acctgcgaat ggttactgta gcagactata cgttcatcgt 24060taaccgtaac gttgttgcac agaagaacac aaagtctgtc aacttaccga attacaaccc 24120taatcaagac ggattgatta acgttcgtgg tggtcagtat ggtagggaac taattgtaca 24180cattaacggt aaagacgttg cgaagtataa gataccagat ggtagtcaac ctgaacacgt 24240aaacaatacg gatgcccaat ggttagctga agagttagcc aagcagatgc gcactaactt 24300gtctgattgg actgtaaatg tagggcaagg gttcatccat gtgaccgcac ctagtggtca 24360acagattgac tccttcacga ctaaagatgg ctacgcagac cagttgatta accctgtgac 24420ccactacgct cagtcgttct ctaagctgcc acctaatgct cctaacggct acatggtgaa 24480aatcgtaggg gacgcctcta agtctgccga ccagtattac gttcggtatg acgctgagcg 24540gaaagtttgg actgagactt taggttggaa cactgaggac caagttctat gggaaaccat 24600gccacacgct cttgtgcgag ccgctgacgg taatttcgac ttcaagtggc ttgagtggtc 24660tcctaagtct tgtggtgacg ttgacaccaa cccttggcct tcttttgttg gttcaagtat 24720taacgatgtg ttcttcttcc gtaaccgctt aggattcctt agtggggaga acatcatatt 24780gagtcgtaca gccaaatact tcaacttcta ccctgcgtcc attgcgaacc ttagtgatga 24840cgaccctata gacgtagctg tgagtaccaa ccgaatagca atccttaagt acgccgttcc 24900gttctcagaa gagttactca tctggtccga tgaagcacaa ttcgtcctga ctgcctcggg 24960tactctcaca tctaagtcgg ttgagttgaa cctaacgacc cagtttgacg tacaggaccg 25020agcgagacct tttgggattg ggcgtaatgt ctactttgct agtccgaggt ccagcttcac 25080gtccatccac aggtactacg ctgtgcagga tgtcagttcc gttaagaatg ctgaggacat 25140tacatcacac gttcctaact acatccctaa tggtgtgttc agtatttgcg gaagtggtac 25200ggaaaacttc tgttcggtac tatctcacgg ggaccctagt aaaatcttca tgtacaaatt 25260cctgtacctg aacgaagagt taaggcaaca gtcgtggtct cattgggact ttggggaaaa 25320cgtacaggtt ctagcttgtc agagtatcag ctcagatatg tatgtgattc ttcgcaatga 25380gttcaatacg ttcctagcta gaatctcttt cactaagaac gccattgact tacagggaga 25440accctatcgt gcctttatgg acatgaagat tcgatacacg attcctagtg gaacatacaa 25500cgatgacaca ttcactacct ctattcatat tccaacaatt tatggtgcaa acttcgggag 25560gggcaaaatc actgtattgg agcctgatgg taagataacc gtgtttgagc aacctacggc 25620tgggtggaat agcgaccctt ggctgagact cagcggtaac ttggagggac gcatggtgta 25680cattgggttc aacattaact tcgtatatga gttctctaag ttcctcatca agcagactgc 25740cgacgacggg tctacctcca cggaagacat tgggcgctta cagttacgcc gagcgtgggt 25800taactacgag aactctggta cgtttgacat ttatgttgag aaccaatcgt ctaactggaa 25860gtacacaatg gctggtgccc gattaggctc taacactctg agggctggga gactgaactt 25920agggaccgga caatatcgat tccctgtggt tggtaacgcc aagttcaaca ctgtatacat 25980cttgtcagat gagactaccc ctctgaacat cattgggtgt ggctgggaag gtaactactt 26040acggagaagt tccggtattt aattaaatat tctccctgtg gtggctcgaa attaatacga 26100ctcactatag ggagaacaat acgactacgg gagggttttc ttatgatgac tataagacct 26160actaaaagta cagactttga ggtattcact ccggctcacc atgacattct tgaagctaag 26220gctgctggta ttgagccgag tttccctgat gcttccgagt gtgtcacgtt gagcctctat 26280gggttccctc tagctatcgg tggtaactgc ggggaccagt gctggttcgt tacgagcgac 26340caagtgtggc gacttagtgg aaaggctaag cgaaagttcc gtaagttaat catggagtat 26400cgcgataaga tgcttgagaa gtatgatact ctttggaatt acgtatgggt aggcaatacg 26460tcccacattc gtttcctcaa gactatcggt gcggtattcc atgaagagta cacacgagat 26520ggtcaatttc agttatttac aatcacgaaa ggaggataac catatgtgtt gggcagccgc 26580aatacctatc gctatatctg gcgctcaggc tatcagtggt cagaacgctc aggccaaaat 26640gattgccgct cagaccgctg ctggtcgtcg tcaagctatg gaaatcatga ggcagacgaa 26700catccagaat gctgacctat cgttgcaagc tcgaagtaaa cttgaggaag cgtccgccga 26760gttgacctca cagaacatgc agaaggtcca agctattggg tctatccgag cggctatcgg 26820agagagtatg cttgaaggtt cctcaatgga ccgcattaag cgagtcacag aaggacagtt 26880cattcgggaa gccaatatgg taactgagaa ctatcgccgt gactaccaag caatcttcgc 26940acagcaactt ggtggtactc aaagtgctgc aagtcagatt gacgaaatct ataagagcga 27000acagaaacag aagagtaagc tacagatggt tctggaccca ctggctatca tggggtcttc 27060cgctgcgagt gcttacgcat ccggtgcgtt cgactctaag tccacaacta aggcacctat 27120tgttgccgct aaaggaacca agacggggag gtaatgagct atgagtaaaa ttgaatctgc 27180ccttcaagcg gcacaaccgg gactctctcg gttacgtggt ggtgctggag gtatgggcta 27240tcgtgcagca accactcagg ccgaacagcc aaggtcaagc ctattggaca ccattggtcg 27300gttcgctaag gctggtgccg atatgtatac cgctaaggaa caacgagcac gagacctagc 27360tgatgaacgc tctaacgaga ttatccgtaa gctgacccct gagcaacgtc gagaagctct 27420caacaacggg acccttctgt atcaggatga cccatacgct atggaagcac tccgagtcaa 27480gactggtcgt aacgctgcgt atcttgtgga cgatgacgtt atgcagaaga taaaagaggg 27540tgtcttccgt actcgcgaag agatggaaga gtatcgccat agtcgccttc aagagggcgc 27600taaggtatac gctgagcagt tcggcatcga ccctgaggac gttgattatc agcgtggttt 27660caacggggac attaccgagc gtaacatctc gctgtatggt gcgcatgata acttcttgag 27720ccagcaagct cagaagggcg ctatcatgaa cagccgagtg gaactcaacg gtgtccttca 27780agaccctgat atgctgcgtc gtccagactc tgctgacttc tttgagaagt atatcgacaa 27840cggtctggtt actggcgcaa tcccatctga tgctcaagcc acacagctta taagccaagc 27900gttcagtgac gcttctagcc gtgctggtgg tgctgacttc ctgatgcgag tcggtgacaa 27960gaaggtaaca cttaacggag ccactacgac ttaccgagag ttgattggtg aggaacagtg 28020gaacgctctc atggtcacag cacaacgttc tcagtttgag actgacgcga agctgaacga 28080gcagtatcgc ttgaagatta actctgcgct gaaccaagag gacccaagga cagcttggga 28140gatgcttcaa ggtatcaagg ctgaactaga taaggtccaa cctgatgagc agatgacacc 28200acaacgtgag tggctaatct ccgcacagga acaagttcag aatcagatga acgcatggac 28260gaaagctcag gccaaggctc tggacgattc catgaagtca atgaacaaac ttgacgtaat 28320cgacaagcaa ttccagaagc gaatcaacgg tgagtgggtc tcaacggatt ttaaggatat 28380gccagtcaac gagaacactg gtgagttcaa gcatagcgat atggttaact acgccaataa 28440gaagctcgct gagattgaca gtatggacat tccagacggt gccaaggatg ctatgaagtt 28500gaagtacctt caagcggact ctaaggacgg agcattccgt acagccatcg gaaccatggt 28560cactgacgct ggtcaagagt ggtctgccgc tgtgattaac ggtaagttac cagaacgaac 28620cccagctatg gatgctctgc gcagaatccg caatgctgac cctcagttga ttgctgcgct 28680atacccagac caagctgagc tattcctgac gatggacatg atggacaagc agggtattga 28740ccctcaggtt attcttgatg ccgaccgact gactgttaag cggtccaaag agcaacgctt 28800tgaggatgat aaagcattcg agtctgcact gaatgcatct aaggctcctg agattgcccg 28860tatgccagcg tcactgcgcg aatctgcacg taagatttat gactccgtta agtatcgctc 28920ggggaacgaa agcatggcta tggagcagat gaccaagttc cttaaggaat ctacctacac 28980gttcactggt gatgatgttg acggtgatac cgttggtgtg attcctaaga atatgatgca 29040ggttaactct gacccgaaat catgggagca aggtcgggat attctggagg aagcacgtaa 29100gggaatcatt gcgagcaacc cttggataac caataagcaa ctgaccatgt attctcaagg 29160tgactccatt taccttatgg acaccacagg tcaagtcaga gtccgatacg acaaagagtt 29220actctcgaag gtctggagtg agaaccagaa gaaactcgaa gagaaagctc gtgagaaggc 29280tctggctgat gtgaacaagc gagcacctat agttgccgct acgaaggccc gtgaagctgc 29340tgctaaacga gtccgagaga aacgtaaaca gactcctaag ttcatctacg gacgtaagga 29400gtaactaaag gctacataag gaggccctaa atggataagt acgataagaa cgtaccaagt 29460gattatgatg gtctgttcca aaaggctgct gatgccaacg gggtctctta tgacctttta 29520cgtaaagtcg cttggacaga atcacgattt gtgcctacag caaaatctaa gactggacca 29580ttaggcatga tgcaatttac caaggcaacc gctaaggccc tcggtctgcg agttaccgat 29640ggtccagacg acgaccgact gaaccctgag ttagctatta atgctgccgc taagcaactt 29700gcaggtctgg tagggaagtt tgatggcgat gaactcaaag ctgcccttgc gtacaaccaa 29760ggcgagggac gcttgggtaa tccacaactt gaggcgtact ctaagggaga cttcgcatca 29820atctctgagg agggacgtaa ctacatgcgt aaccttctgg atgttgctaa gtcacctatg 29880gctggacagt tggaaacttt tggtggcata accccaaagg gtaaaggcat tccggctgag 29940gtaggattgg ctggaattgg tcacaagcag aaagtaacac aggaacttcc tgagtccaca 30000agttttgacg ttaagggtat cgaacaggag gctacggcga aaccattcgc caaggacttt 30060tgggagaccc acggagaaac acttgacgag tacaacagtc gttcaacctt cttcggattc 30120aaaaatgctg ccgaagctga actctccaac tcagtcgctg ggatggcttt ccgtgctggt 30180cgtctcgata atggttttga tgtgtttaaa gacaccatta cgccgactcg ctggaactct 30240cacatctgga ctccagagga gttagagaag attcgaacag aggttaagaa ccctgcgtac 30300atcaacgttg taactggtgg ttcccctgag aacctcgatg acctcattaa attggctaac 30360gagaactttg agaatgactc ccgcgctgcc gaggctggcc taggtgccaa actgagtgct 30420ggtattattg gtgctggtgt ggacccgctt agctatgttc ctatggtcgg tgtcactggt 30480aagggcttta agttaatcaa taaggctctt gtagttggtg ccgaaagtgc tgctctgaac 30540gttgcatccg aaggtctccg tacctccgta gctggtggtg acgcagacta tgcgggtgct 30600gccttaggtg gctttgtgtt tggcgcaggc atgtctgcaa tcagtgacgc tgtagctgct 30660ggactgaaac gcagtaaacc agaagctgag ttcgacaatg agttcatcgg tcctatgatg 30720cgattggaag cccgtgagac agcacgaaac gccaactctg cggacctctc tcggatgaac 30780actgagaaca tgaagtttga aggtgaacat aatggtgtcc cttatgagga cttaccaaca 30840gagagaggtg ccgtggtgtt acatgatggc tccgttctaa gtgcaagcaa cccaatcaac 30900cctaagactc taaaagagtt ctccgaggtt gaccctgaga aggctgcgcg aggaatcaaa 30960ctggctgggt tcaccgagat tggcttgaag accttggggt ctgacgatgc tgacatccgt 31020agagtggcta tcgacctcgt tcgctctcct actggtatgc agtctggtgc ctcaggtaag 31080ttcggtgcaa cagcttctga catccatgag agacttcatg gtactgacca gcgtacttat 31140aatgacttgt acaaagcaat gtctgacgct atgaaagacc ctgagttctc tactggcggc 31200gctaagatgt cccgtgaaga aactcgatac actatctacc gtagagcggc actagctatt 31260gagcgtccag aactacagaa ggcactcact ccgtctgaga gaatcgttat ggacatcatt 31320aagcgtcact ttgacaccaa gcgtgaactt atggaaaacc cagcaatatt cggtaacaca 31380aaggctgtga gtatcttccc tgagagtcgc cacaaaggta cttacgttcc tcacgtatat 31440gaccgtcatg ccaaggcgct gatgattcaa cgctacggtg ccgaaggttt gcaggaaggg 31500attgcccgct catggatgaa cagctacgtc tccagacctg aggtcaaggc cagagtcgat 31560gagatgctta aggaattaca cggggtgaag gaagtaacac cagagatggt agagaagtac 31620gctatggata aggcttatgg tatctcccac tcagaccagt tcaccaacag ttccataata 31680gaagagaaca ttgagggctt agtaggtatc gagaataact cattccttga ggcacgtaac 31740ttgtttgatt cggacctatc catcactatg ccagacggac agcaattctc agtgaatgac 31800ctaagggact tcgatatgtt ccgcatcatg ccagcgtatg accgccgtgt caatggtgac 31860atcgccatca tggggtctac tggtaaaacc actaaggaac ttaaggatga gattttggct 31920ctcaaagcga aagctgaggg agacggtaag aagactggcg aggtacatgc tttaatggat 31980accgttaaga ttcttactgg tcgtgctaga cgcaatcagg acactgtgtg ggaaacctca 32040ctgcgtgcca tcaatgacct agggttcttc gctaagaacg cctacatggg tgctcagaac 32100attacggaga ttgctgggat gattgtcact ggtaacgttc gtgctctagg gcatggtatc 32160ccaattctgc gtgatacact ctacaagtct aaaccagttt cagctaagga actcaaggaa 32220ctccatgcgt ctctgttcgg gaaggaggtg gaccagttga ttcggcctaa acgtgctgac 32280attgtgcagc gcctaaggga agcaactgat accggacctg ccgtggcgaa catcgtaggg 32340accttgaagt attcaacaca ggaactggct gctcgctctc cgtggactaa gctactgaac 32400ggaaccacta actaccttct ggatgctgcg cgtcaaggta tgcttgggga tgttattagt 32460gccaccctaa caggtaagac tacccgctgg gagaaagaag gcttccttcg tggtgcctcc 32520gtaactcctg agcagatggc tggcatcaag tctctcatca aggaacatat ggtacgcggt 32580gaggacggga agtttaccgt taaggacaag caagcgttct ctatggaccc acgggctatg 32640gacttatgga gactggctga caaggtagct gatgaggcaa tgctgcgtcc acataaggtg 32700tccttacagg attcccatgc gttcggagca ctaggtaaga tggttatgca gtttaagtct 32760ttcactatca agtcccttaa ctctaagttc ctgcgaacct tctatgatgg atacaagaac 32820aaccgagcga ttgacgctgc gctgagcatc atcacctcta tgggtctcgc tggtggtttc 32880tatgctatgg ctgcacacgt caaagcatac gctctgccta aggagaaacg taaggagtac 32940ttggagcgtg cactggaccc aaccatgatt gcccacgctg cgttatctcg tagttctcaa 33000ttgggtgctc ctttggctat ggttgaccta gttggtggtg ttttagggtt cgagtcctcc 33060aagatggctc gctctacgat tctacctaag gacaccgtga aggaacgtga cccaaacaaa 33120ccgtacacct ctagagaggt aatgggcgct atgggttcaa accttctgga acagatgcct 33180tcggctggct ttgtggctaa cgtaggggct accttaatga atgctgctgg cgtggtcaac 33240tcacctaata aagcaaccga gcaggacttc atgactggtc ttatgaactc cacaaaagag 33300ttagtaccga acgacccatt gactcaacag cttgtgttga agatttatga ggcgaacggt 33360gttaacttga gggagcgtag gaaataatac gactcactat agggagaggc gaaataatct 33420tctccctgta gtctcttaga tttactttaa ggaggtcaaa tggctaacgt aattaaaacc 33480gttttgactt accagttaga tggctccaat cgtgatttta atatcccgtt tgagtatcta 33540gcccgtaagt tcgtagtggt aactcttatt ggtgtagacc gaaaggtcct tacgattaat 33600acagactatc gctttgctac acgtactact atctctctga caaaggcttg gggtccagcc 33660gatggctaca cgaccatcga gttacgtcga gtaacctcca ctaccgaccg attggttgac 33720tttacggatg gttcaatcct ccgcgcgtat gaccttaacg tcgctcagat tcaaacgatg 33780cacgtagcgg aagaggcccg tgacctcact acggatacta tcggtgtcaa taacgatggt 33840cacttggatg ctcgtggtcg tcgaattgtg aacctagcga acgccgtgga tgaccgcgat 33900gctgttccgt ttggtcaact aaagaccatg aaccagaact catggcaagc acgtaatgaa 33960gccttacagt tccgtaatga

ggctgagact ttcagaaacc aagcggaggg ctttaagaac 34020gagtccagta ccaacgctac gaacacaaag cagtggcgcg atgagaccaa gggtttccga 34080gacgaagcca agcggttcaa gaatacggct ggtcaatacg ctacatctgc tgggaactct 34140gcttccgctg cgcatcaatc tgaggtaaac gctgagaact ctgccacagc atccgctaac 34200tctgctcatt tggcagaaca gcaagcagac cgtgcggaac gtgaggcaga caagctggaa 34260aattacaatg gattggctgg tgcaattgat aaggtagatg gaaccaatgt gtactggaaa 34320ggaaatattc acgctaacgg gcgcctttac atgaccacaa acggttttga ctgtggccag 34380tatcaacagt tctttggtgg tgtcactaat cgttactctg tcatggagtg gggagatgag 34440aacggatggc tgatgtatgt tcaacgtaga gagtggacaa cagcgatagg cggtaacatc 34500cagttagtag taaacggaca gatcatcacc caaggtggag ccatgaccgg tcagctaaaa 34560ttgcagaatg ggcatgttct tcaattagag tccgcatccg acaaggcgca ctatattcta 34620tctaaagatg gtaacaggaa taactggtac attggtagag ggtcagataa caacaatgac 34680tgtaccttcc actcctatgt acatggtacg accttaacac tcaagcagga ctatgcagta 34740gttaacaaac acttccacgt aggtcaggcc gttgtggcca ctgatggtaa tattcaaggt 34800actaagtggg gaggtaaatg gctggatgct tacctacgtg acagcttcgt tgcgaagtcc 34860aaggcgtgga ctcaggtgtg gtctggtagt gctggcggtg gggtaagtgt gactgtttca 34920caggatctcc gcttccgcaa tatctggatt aagtgtgcca acaactcttg gaacttcttc 34980cgtactggcc ccgatggaat ctacttcata gcctctgatg gtggatggtt acgattccaa 35040atacactcca acggtctcgg attcaagaat attgcagaca gtcgttcagt acctaatgca 35100atcatggtgg agaacgagta attggtaaat cacaaggaaa gacgtgtagt ccacggatgg 35160actctcaagg aggtacaagg tgctatcatt agactttaac aacgaattga ttaaggctgc 35220tccaattgtt gggacgggtg tagcagatgt tagtgctcga ctgttctttg ggttaagcct 35280taacgaatgg ttctacgttg ctgctatcgc ctacacagtg gttcagattg gtgccaaggt 35340agtcgataag atgattgact ggaagaaagc caataaggag tgatatgtat ggaaaaggat 35400aagagcctta ttacattctt agagatgttg gacactgcga tggctcagcg tatgcttgcg 35460gacctttcgg accatgagcg tcgctctccg caactctata atgctattaa caaactgtta 35520gaccgccaca agttccagat tggtaagttg cagccggatg ttcacatctt aggtggcctt 35580gctggtgctc ttgaagagta caaagagaaa gtcggtgata acggtcttac ggatgatgat 35640atttacacat tacagtgata tactcaaggc cactacagat agtggtcttt atggatgtca 35700ttgtctatac gagatgctcc tacgtgaaat ctgaaagtta acgggaggca ttatgctaga 35760atttttacgt aagctaatcc cttgggttct cgctgggatg ctattcgggt taggatggca 35820tctagggtca gactcaatgg acgctaaatg gaaacaggag gtacacaatg agtacgttaa 35880gagagttgag gctgcgaaga gcactcaaag agcaatcgat gcggtatctg ctaagtatca 35940agaagacctt gccgcgctgg aagggagcac tgataggatt atttctgatt tgcgtagcga 36000caataagcgg ttgcgcgtca gagtcaaaac taccggaacc tccgatggtc agtgtggatt 36060cgagcctgat ggtcgagccg aacttgacga ccgagatgct aaacgtattc tcgcagtgac 36120ccagaagggt gacgcatgga ttcgtgcgtt acaggatact attcgtgaac tgcaacgtaa 36180gtaggaaatc aagtaaggag gcaatgtgtc tactcaatcc aatcgtaatg cgctcgtagt 36240ggcgcaactg aaaggagact tcgtggcgtt cctattcgtc ttatggaagg cgctaaacct 36300accggtgccc actaagtgtc agattgacat ggctaaggtg ctggcgaatg gagacaacaa 36360gaagttcatc ttacaggctt tccgtggtat cggtaagtcg ttcatcacat gtgcgttcgt 36420tgtgtggtcc ttatggagag accctcagtt gaagatactt atcgtatcag cctctaagga 36480gcgtgcagac gctaactcca tctttattaa gaacatcatt gacctgctgc cattcctatc 36540tgagttaaag ccaagacccg gacagcgtga ctcggtaatc agctttgatg taggcccagc 36600caatcctgac cactctccta gtgtgaaatc agtaggtatc actggtcagt taactggtag 36660ccgtgctgac attatcattg cggatgacgt tgagattccg tctaacagcg caactatggg 36720tgcccgtgag aagctatgga ctctggttca ggagttcgct gcgttactta aaccgctgcc 36780ttcctctcgc gttatctacc ttggtacacc tcagacagag atgactctct ataaggaact 36840tgaggataac cgtgggtaca caaccattat ctggcctgct ctgtacccaa ggacacgtga 36900agagaacctc tattactcac agcgtcttgc tcctatgtta cgcgctgagt acgatgagaa 36960ccctgaggca cttgctggga ctccaacaga cccagtgcgc tttgaccgtg atgacctgcg 37020cgagcgtgag ttggaatacg gtaaggctgg ctttacgcta cagttcatgc ttaaccctaa 37080ccttagtgat gccgagaagt acccgctgag gcttcgtgac gctatcgtag cggccttaga 37140cttagagaag gccccaatgc attaccagtg gcttccgaac cgtcagaaca tcattgagga 37200ccttcctaac gttggcctta agggtgatga cctgcatacg taccacgatt gttccaacaa 37260ctcaggtcag taccaacaga agattctggt cattgaccct agtggtcgcg gtaaggacga 37320aacaggttac gctgtgctgt acacactgaa cggttacatc taccttatgg aagctggagg 37380tttccgtgat ggctactccg ataagaccct tgagttactc gctaagaagg caaagcaatg 37440gggagtccag acggttgtct acgagagtaa cttcggtgac ggtatgttcg gtaaggtatt 37500cagtcctatc cttcttaaac accacaactg tgcgatggaa gagattcgtg cccgtggtat 37560gaaagagatg cgtatttgcg atacccttga gccagtcatg cagactcacc gccttgtaat 37620tcgtgatgag gtcattaggg ccgactacca gtccgctcgt gacgtagacg gtaagcatga 37680cgttaagtac tcgttgttct accagatgac ccgtatcact cgtgagaaag gcgctctggc 37740tcatgatgac cgattggatg cccttgcgtt aggcattgag tatctccgtg agtccatgca 37800gttggattcc gttaaggtcg agggtgaagt acttgctgac ttccttgagg aacacatgat 37860gcgtcctacg gttgctgcta cgcatatcat tgagatgtct gtgggaggag ttgatgtgta 37920ctctgaggac gatgagggtt acggtacgtc tttcattgag tggtgattta tgcattagga 37980ctgcataggg atgcactata gaccacggat ggtcagttct ttaagttact gaaaagacac 38040gataaattaa tacgactcac tatagggaga ggagggacga aaggttacta tatagatact 38100gaatgaatac ttatagagtg cataaagtat gcataatggt gtacctagag tgacctctaa 38160gaatggtgat tatattgtat tagtatcacc ttaacttaag gaccaacata aagggaggag 38220actcatgttc cgcttattgt tgaacctact gcggcataga gtcacctacc gatttcttgt 38280ggtactttgt gctgcccttg ggtacgcatc tcttactgga gacctcagtt cactggagtc 38340tgtcgtttgc tctatactca cttgtagcga ttagggtctt cctgaccgac tgatggctca 38400ccgagggatt cagcggtatg attgcatcac accacttcat ccctatagag tcaagtccta 38460aggtataccc ataaagagcc tctaatggtc tatcctaagg tctataccta aagataggcc 38520atcctatcag tgtcacctaa agagggtctt agagagggcc tatggagttc ctatagggtc 38580ctttaaaata taccataaaa atctgagtga ctatctcaca gtgtacggac ctaaagttcc 38640cccatagggg gtacctaaag cccagccaat cacctaaagt caaccttcgg ttgaccttga 38700gggttcccta agggttgggg atgacccttg ggtttgtctt tgggtgttac cttgagtgtc 38760tctctgtgtc cct 38773344507DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 3gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg 60ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg 120acaggtgctg aaagcgaggc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt 180ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg 240taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg gcaagggtaa 300tgaggtgctt tatgactctg ccgccgtcat aaaatggtat gccgaaaggg atgctgaaat 360tgagaacgaa aagctgcgcc gggaggttga agaactgcgg caggccagcg aggcagatct 420ccagccagga actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca 480ggaactgaag aatgccagag actccgctga agtggtggaa accgcattct gtactttcgt 540gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggctccccc tgtcggtgca 600gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa 660agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat 720cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca 780ggccggagcc acagaccgcc gttgaatggg cggatgctaa ttactatctc ccgaaagaat 840ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga 900tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca 960aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac acccttatct 1020ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgactattc 1080gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac cgggataaca 1140cgctcaccat gaagcgtttc actaatgggc gtggcttctg gtgcctgggc ggtaaagcgg 1200caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcttttg 1260atgatgatat tgaacaggaa ggctctccga cgttcctggg tgacaagcgt attgaaggct 1320cggtctggcc aaagtccatc cgtggctcca cgccaaaagt gagaggcacc tgtcagattg 1380agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcattgcg 1440gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc aaatggacgc 1500cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgccagc 1560aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg 1620atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac agtgtgacct 1680ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga 1740tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga 1800cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc 1860attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc 1920tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg 1980accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg 2040ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct 2100gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaaa catgggctgt 2160tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc agcatgccac 2220gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc 2280agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc 2340acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg actgctgaag 2400agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc aaaaagcgac 2460gcaatgaggc actcgactgc ttcgtttatg cgctggcggc gctgcgcatc agtatttccc 2520gctggcagct ggatctcagt gcgctgctgg cgagcctgca ggaagaggat ggtgcagcaa 2580ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat gaatgacgcg 2640acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag gtaaacgggt 2700ggcaacagta cagaaagacg gacgaagggt ggagtttacg gccacttccg tgtctgacct 2760gaaaaaatat attgcagagc tggaagtgca gaccggcatg acacagcgac gcaggggacc 2820tgcaggattt tatgtatgaa aacgcccacc attcccaccc ttctggggcc ggacggcatg 2880acatcgctgc gcgaatatgc cggttatcac ggcggtggca gcggatttgg agggcagttg 2940cggtcgtgga acccaccgag tgaaagtgtg gatgcagccc tgttgcccaa ctttacccgt 3000ggcaatgccc gcgcagacga tctggtacgc aataacggct atgccgccaa cgccatccag 3060ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc 3120tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg 3180aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc 3240atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc 3300acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag 3360cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt 3420aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg 3480ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcctc gttcattcac 3540gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg 3600gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc cattgtgaag 3660gcgatgtatg ccgccaccat tgagagtgag ctggatacgc agtcagcgat ggattttatt 3720ctgggcgcga acagtcagga gcagcgggaa aggctgaccg gctggattgg tgaaattgcc 3780gcgtattacg ccgcagcgcc ggtccggctg ggaggcgcaa aagtaccgca cctgatgccg 3840ggtgactcac tgaacctgca gacggctcag gatacggata acggctactc cgtgtttgag 3900cagtcactgc tgcggtatat cgctgccggg ctgggtgtct cgtatgagca gctttcccgg 3960aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc gtgggcgtac 4020tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg 4080ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt 4140caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg tatggccatc 4200gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact gagtacctac 4260gagaaagagt gcgcaaaacg cggtgacgac tatcaggaaa tttttgccca gcaggtccgt 4320gaaacgatgg agcgccgtgc agccggtctt aaaccgcccg cctgggcggc tgcagcattt 4380gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc tgcgtaatct 4440cccgcatatt gccagcatgg cctttaatga gccgctgatg cttgaacccg cctatgcgcg 4500ggttttcttt tgtgcgcttg caggccagct tgggatcagc agcctgacgg atgcggtgtc 4560cggcgacagc ctgactgccc aggaggcact cgcgacgctg gcattatccg gtgatgatga 4620cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc tgccggtgtc 4680cggcacgctg gtcagccgga cgcgggcgct gcagccgtac tcggggatga ccggttacaa 4740cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg gcattctgct 4800cgatatggac acgcccggcg ggatggtggc gggggcattt gactgcgctg acatcatcgc 4860ccgtgtgcgt gacataaaac cggtatgggc gcttgccaac gacatgaact gcagtgcagg 4920tcagttgctt gccagtgccg cctcccggcg tctggtcacg cagaccgccc ggacaggctc 4980catcggcgtc atgatggctc acagtaatta cggtgctgcg ctggagaaac agggtgtgga 5040aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca gccatcttcc 5100ggatgacgtc cgggagacac tgcagtcccg gatggacgca acccgccaga tgtttgcgca 5160gaaggtgtcg gcatataccg gcctgtccgt gcaggttgtg ctggataccg aggctgcagt 5220gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta acagcaccga 5280tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct caggagggcg 5340aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg ctgacgttac 5400tgacgtggtg ccagcgacgg agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc 5460gcagatcacc gcagcggttg cggcagaaaa cagccgcatt atggggatcc tcaactgtga 5520ggaggctcac ggacgcgaag aacaggcacg cgtgctggca gaaacccccg gtatgaccgt 5580gaaaacggcc cgccgcattc tggccgcagc accacagagt gcacaggcgc gcagtgacac 5640tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta acccggcatc 5700tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga cgagcaaaga 5760aacctttacc cattaccagc cgcagggcaa cagtgacccg gctcataccg caaccgcgcc 5820cctagacctt catcactaaa ggccgcctgt gcggcttttt ttacgggatt tttttatgtc 5880gatgtacaca accgcccaac tgctggcggc aaatgagcag aaatttaagt ttgatccgct 5940gtttctgcgt ctctttttcc gtgagagcta tcccttcacc acggagaaag tctatctctc 6000acaaattccg ggactggtaa acatggcgct gtacgtttcg ccgattgttt ccggtgaggt 6060tatccgttcc cgtggcggct ccacctctga atttacgccg ggatatgtca agccgaagca 6120tgaagtgaat ccgcagatga ccctgcgtcg cctgccggat gaagatccgc agaatctggc 6180ggacccggct taccgccgcc gtcgcatcat catgcagaac atgcgtgacg aagagctggc 6240cattgctcag gtcgaagaga tgcaggcagt ttctgccgtg cttaagggca aatacaccat 6300gaccggtgaa gccttcgatc cggttgaggt ggatatgggc cgcagtgagg agaataacat 6360cacgcagtcc ggcggcacgg agtggagcaa gcgtgacaag tccacgtatg acccgaccga 6420cgatatcgaa gcctacgcgc tgaacgccag cggtgtggtg aatatcatcg tgttcgatcc 6480gaaaggctgg gcgctgttcc gttccttcaa agccgtcaag gagaagctgg atacccgtcg 6540tggctctaat tccgagctgg agacagcggt gaaagacctg ggcaaagcgg tgtcctataa 6600ggggatgtat ggcgatgtgg ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt 6660caaaaagaac ttcctgccgg acaacacgat ggtgctgggg aacactcagg cacgcggtct 6720gcgcacctat ggctgcattc aggatgcgga cgcacagcgc gaaggcatta acgcctctgc 6780ccgttacccg aaaaactggg tgaccaccgg cgatccggcg cgtgagttca ccatgattca 6840gtcagcaccg ctgatgctgc tggctgaccc tgatgagttc gtgtccgtac aactggcgta 6900atcatggccc ttcggggcca ttgtttctct gtggaggagt ccatgacgaa agatgaactg 6960attgcccgtc tccgctcgct gggtgaacaa ctgaaccgtg atgtcagcct gacggggacg 7020aaagaagaac tggcgctccg tgtggcagag ctgaaagagg agcttgatga cacggatgaa 7080actgccggtc aggacacccc tctcagccgg gaaaatgtgc tgaccggaca tgaaaatgag 7140gtgggatcag cgcagccgga taccgtgatt ctggatacgt ctgaactggt cacggtcgtg 7200gcactggtga agctgcatac tgatgcactt cacgccacgc gggatgaacc tgtggcattt 7260gtgctgccgg gaacggcgtt tcgtgtctct gccggtgtgg cagccgaaat gacagagcgc 7320ggcctggcca gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg 7380ctgccattgc ccgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatta 7440catccggtga gcagtcaggt gcggtgatac gtggtgtttt tgatgaccct gaaaatatca 7500gctatgccgg acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg 7560atgaggtgcg gcagctgcgg cgtggagaca cgctgaccat cggtgaggaa aatttctggg 7620tagatcgggt ttcgccggat gatggcggaa gttgtcatct ctggcttgga cggggcgtac 7680cgcctgccgt taaccgtcgc cgctgaaagg gggatgtatg gccataaaag gtcttgagca 7740ggccgttgaa aacctcagcc gtatcagcaa aacggcggtg cctggtgccg ccgcaatggc 7800cattaaccgc gttgcttcat ccgcgatatc gcagtcggcg tcacaggttg cccgtgagac 7860aaaggtacgc cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa 7920tccgcaggcc agaatcaaag ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc 7980gcgggttgtc ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg 8040tggcggcagc gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact 8100gaaaaatggc cggtggcatg tcatgcagcg tgtggctggg aaaaaccgtt accccattga 8160tgtggtgaaa atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg 8220gatacggcgt gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat 8280ggtaataaag cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag 8340catgacaccg gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt 8400ccggcagttg ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat 8460acctggcagg cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag 8520ctggatgcgt ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca 8580gatttgatca ccagtatggt ggccagcggc tatgactacc ggcgcgacga tgatgcgggc 8640ttgtggagtt cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc 8700tgtaccaaat cctacaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg 8760gagcggtgac ccttacgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt 8820taaagacctg acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga 8880tgaagatgca gactggactg cgaccgggca ggggcagaaa tctgccggag ataccagctt 8940cacgctggcg tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga 9000aggcgatacc cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg 9060ctgggtcagc agtatcggta aggcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa 9120agtcaccaat gtgggacgtc cgtcgatggc agaagatcgc agcacggtaa cagcggcaac 9180cggcatgacc gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac 9240cgtggccttc cagccggagg gcgtaaccga caagagcttt cgtgcggtgt ctgcggataa 9300aacaaaagcc accgtgtcgg tcagtggtat gaccatcacc gtgaacggcg ttgctgcagg 9360caaggtcaac attccggttg tatccggtaa tggtgagttt gctgcggttg cagaaattac 9420cgtcaccgcc agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca 9480taacggtgtg accgtcacgc tttctgaact gtcagccctg cagcgcattg agcatctcgc 9540cctgatgaaa cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttactgtgga 9600agacgccatc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccgca 9660gaagacgcag atgccgtcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac 9720cacctggccc acggaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtctggtat 9780gtatgagttt gtggtgaata atgcccctga acagacagag gacgccgggc ccgcagagcc 9840tgtttctgcg ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgtgag 9900atggggcgac ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac 9960tggcaccgct tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc 10020gggctgacgt acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat 10080ttcagtctgc tgaaccggcg cgaggctgac gaagagcctg aagatgatgt gctgatgcag

10140aaagcggcag ggcttgccgg aggtgtccgc tttggcccgg acgggaatga agttatcccc 10200gcttccccgg atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa 10260gggatcgcag gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttgag 10320tctggatgcg gccagatttg acgagcagat ggccagagtc aggcgtcatt tttctggtac 10380ggaaagtgat gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct 10440ggctgcacag aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc 10500tgcacagttc accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct 10560gctgcaacag ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg 10620gcttgccggt gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg 10680tgcgctggcg tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct 10740ggtcctttcc ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc 10800cgggcaggcg gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa 10860ggcgggggta agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc 10920ctctgcatcc ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga 10980cccgacgtcg gggctgacgg cgatggctcg ccagttccat aacgtgtcgg cggagcagat 11040tgcgtatgtt gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa 11100cgaggccgca acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac 11160gctggagacc tgggcagaca ggactgcgcg ggcattcaaa tccatgtggg atgcggtgct 11220ggatattggt cgtcctgata ccgcgcagga gatgctgatt aaggcagagg ctgcgtataa 11280gaaagcagac gacatctgga atctgcgcaa ggatgattat tttgttaacg atgaagcgcg 11340ggcgcgttac tgggatgatc gtgaaaaggc ccgtcttgcg cttgaagccg cccgaaagaa 11400ggctgagcag cagactcaac aggacaaaaa tgcgcagcag cagagcgata ccgaagcgtc 11460acggctgaaa tataccgaag aggcgcagaa ggcttacgaa cggctgcaga cgccgctgga 11520gaaatatacc gcccgtcagg aagaactgaa caaggcactg aaagacggga aaatcctgca 11580ggcggattac aacacgctga tggcggcggc gaaaaaggat tatgaagcga cgctgaaaaa 11640gccgaaacag tccagcgtga aggtgtctgc gggcgatcgt caggaagaca gtgctcatgc 11700tgccctgctg acgcttcagg cagaactccg gacgctggag aagcatgccg gagcaaatga 11760gaaaatcagc cagcagcgcc gggatttgtg gaaggcggag agtcagttcg cggtactgga 11820ggaggcggcg caacgtcgcc agctgtctgc acaggagaaa tccctgctgg cgcataaaga 11880tgagacgctg gagtacaaac gccagctggc tgcacttggc gacaaggtta cgtatcagga 11940gcgcctgaac gcgctggcgc agcaggcgga taaattcgca cagcagcaac gggcaaaacg 12000ggccgccatt gatgcgaaaa gccgggggct gactgaccgg caggcagaac gggaagccac 12060ggaacagcgc ctgaaggaac agtatggcga taatccgctg gcgctgaata acgtcatgtc 12120agagcagaaa aagacctggg cggctgaaga ccagcttcgc gggaactgga tggcaggcct 12180gaagtccggc tggagtgagt gggaagagag cgccacggac agtatgtcgc aggtaaaaag 12240tgcagccacg cagacctttg atggtattgc acagaatatg gcggcgatgc tgaccggcag 12300tgagcagaac tggcgcagct tcacccgttc cgtgctgtcc atgatgacag aaattctgct 12360taagcaggca atggtgggga ttgtcgggag tatcggcagc gccattggcg gggctgttgg 12420tggcggcgca tccgcgtcag gcggtacagc cattcaggcc gctgcggcga aattccattt 12480tgcaaccgga ggatttacgg gaaccggcgg caaatatgag ccagcgggga ttgttcaccg 12540tggtgagttt gtcttcacga aggaggcaac cagccggatt ggcgtgggga atctttaccg 12600gctgatgcgc ggctatgcca ccggcggtta tgtcggtaca ccgggcagca tggcagacag 12660ccggtcgcag gcgtccggga cgtttgagca gaataaccat gtggtgatta acaacgacgg 12720cacgaacggg cagataggtc cggctgctct gaaggcggtg tatgacatgg cccgcaaggg 12780tgcccgtgat gaaattcaga cacagatgcg tgatggtggc ctgttctccg gaggtggacg 12840atgaagacct tccgctggaa agtgaaaccc ggtatggatg tggcttcggt cccttctgta 12900agaaaggtgc gctttggtga tggctattct cagcgagcgc ctgccgggct gaatgccaac 12960ctgaaaacgt acagcgtgac gctttctgtc ccccgtgagg aggccacggt actggagtcg 13020tttctggaag agcacggggg ctggaaatcc tttctgtgga cgccgcctta tgagtggcgg 13080cagataaagg tgacctgcgc aaaatggtcg tcgcgggtca gtatgctgcg tgttgagttc 13140agcgcagagt ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg 13200aatgcacccg tgcggagcag tcggccagcg tggtgctctg ggaaatcgac ctgacagagg 13260tcggtggaga acgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct 13320ggcaggggcg acagtatcag ccgtatccca ttcaggggag cggttttgaa ctgaatggca 13380aaggcaccag tacgcgcccc acgctgacgg tttctaacct gtacggtatg gtcaccggga 13440tggcggaaga tatgcagagt ctggtcggcg gaacggtggt ccggcgtaag gtttacgccc 13500gttttctgga tgcggtgaac ttcgtcaacg gaaacagtta cgccgatccg gagcaggagg 13560tgatcagccg ctggcgcatt gagcagtgca gcgaactgag cgcggtgagt gcctcctttg 13620tactgtccac gccgacggaa acggatggcg ctgtttttcc gggacgtatc atgctggcca 13680acacctgcac ctggacctat cgcggtgacg agtgcggtta tagcggtccg gctgtcgcgg 13740atgaatatga ccagccaacg tccgatatca cgaaggataa atgcagcaaa tgcctgagcg 13800gttgtaagtt ccgcaataac gtcggcaact ttggcggctt cctttccatt aacaaacttt 13860cgcagtaaat cccatgacac agacagaatc agcgattctg gcgcacgccc ggcgatgtgc 13920gccagcggag tcgtgcggct tcgtggtaag cacgccggag ggggaaagat atttcccctg 13980cgtgaatatc tccggtgagc cggaggctat ttccgtatgt cgccggaaga ctggctgcag 14040gcagaaatgc agggtgagat tgtggcgctg gtccacagcc accccggtgg tctgccctgg 14100ctgagtgagg ccgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc 14160cgggggacga ttcataagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac 14220ggtgtgacgg actgttacac actgttccgg gatgcttatc atctggcggg gattgagatg 14280ccggactttc atcgtgagga tgactggtgg cgtaacggcc agaatctcta tctggataat 14340ctggaggcga cggggctgta tcaggtgccg ttgtcagcgg cacagccggg cgatgtgctg 14400ctgtgctgtt ttggttcatc agtgccgaat cacgccgcaa tttactgcgg cgacggcgag 14460ctgctgcacc atattcctga acaactgagc aaacgagaga ggtacaccga caaatggcag 14520cgacgcacac actccctctg gcgtcaccgg gcatggcgcg catctgcctt tacggggatt 14580tacaacgatt tggtcgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccggg 14640cactggccac acagctcccg gcgtttcgtc agaaactgag cgacggctgg tatcaggtac 14700ggattgccgg gcgggacgtc agcacgtccg ggttaacggc gcagttacat gagactctgc 14760ctgatggcgc tgtaattcat attgttccca gagtcgccgg ggccaagtca ggtggcgtat 14820tccagattgt cctgggggct gccgccattg ccggatcatt ctttaccgcc ggagccaccc 14880ttgcagcatg gggggcagcc attggggccg gtggtatgac cggcatcctg ttttctctcg 14940gtgccagtat ggtgctcggt ggtgtggcgc agatgctggc accgaaagcc agaactcccc 15000gtatacagac aacggataac ggtaagcaga acacctattt ctcctcactg gataacatgg 15060ttgcccaggg caatgttctg cctgttctgt acggggaaat gcgcgtgggg tcacgcgtgg 15120tttctcagga gatcagcacg gcagacgaag gggacggtgg tcaggttgtg gtgattggtc 15180gctgatgcaa aatgttttat gtgaaaccgc ctgcgggcgg ttttgtcatt tatggagcgt 15240gaggaatggg taaaggaagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga 15300agtccacgca gttgctgagt gtgatcgatg ccatcagcga agggccgatt gaaggtccgg 15360tggatggctt aaaaagcgtg ctgctgaaca gtacgccggt gctggacact gaggggaata 15420ccaacatatc cggtgtcacg gtggtgttcc gggctggtga gcaggagcag actccgccgg 15480agggatttga atcctccggc tccgagacgg tgctgggtac ggaagtgaaa tatgacacgc 15540cgatcacccg caccattacg tctgcaaaca tcgaccgtct gcgctttacc ttcggtgtac 15600aggcactggt ggaaaccacc tcaaagggtg acaggaatcc gtcggaagtc cgcctgctgg 15660ttcagataca acgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa 15720ccacctcgca gtatctggcc tcggtggtga tgggtaacct gccgccgcgc ccgtttaata 15780tccggatgcg caggatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct 15840ggtcgtcata cactgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg 15900gcgtgcaggt ggactcggag cagttcggca gccagcaggt gagccgtaat tatcatctgc 15960gcgggcgtat tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta 16020tctgggacgg aacgtttaaa ccggcataca gcaacaacat ggcctggtgt ctgtgggata 16080tgctgaccca tccgcgctac ggcatgggga aacgtcttgg tgcggcggat gtggataaat 16140gggcgctgta tgtcatcggc cagtactgcg accagtcagt gccggacggc tttggcggca 16200cggagccgcg catcacctgt aatgcgtacc tgaccacaca gcgtaaggcg tgggatgtgc 16260tcagcgattt ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt 16320tcgtgcagga ccgaccgtcg gataagacgt ggacctataa ccgcagtaat gtggtgatgc 16380cggatgatgg cgcgccgttc cgctacagct tcagcgccct gaaggaccgc cataatgccg 16440ttgaggtgaa ctggattgac ccgaacaacg gctgggagac ggcgacagag cttgttgaag 16500atacgcaggc cattgcccgt tacggtcgta atgttacgaa gatggatgcc tttggctgta 16560ccagccgggg gcaggcacac cgcgccgggc tgtggctgat taaaacagaa ctgctggaaa 16620cgcagaccgt ggatttcagc gtcggcgcag aagggcttcg ccatgtaccg ggcgatgtta 16680ttgaaatctg cgatgatgac tatgccggta tcagcaccgg tggtcgtgtg ctggcggtga 16740acagccagac ccggacgctg acgctcgacc gtgaaatcac gctgccatcc tccggtaccg 16800cgctgataag cctggttgac ggaagtggca atccggtcag cgtggaggtt cagtccgtca 16860ccgacggcgt gaaggtaaaa gtgagccgtg ttcctgacgg tgttgctgaa tacagcgtat 16920gggagctgaa gctgccgacg ctgcgccagc gactgttccg ctgcgtgagt atccgtgaga 16980acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg 17040tggataacgg ggcgcacttt gacggcgaac agagtggcac ggtgaatggt gtcacgccgc 17100cagcggtgca gcacctgacc gcagaagtca ctgcagacag cggggaatat caggtgctgg 17160cgcgatggga cacaccgaag gtggtgaagg gcgtgagttt cctgctccgt ctgaccgtaa 17220cagcggacga cggcagtgag cggctggtca gcacggcccg gacgacggaa accacatacc 17280gcttcacgca actggcgctg gggaactaca ggctgacagt ccgggcggta aatgcgtggg 17340ggcagcaggg cgatccggcg tcggtatcgt tccggattgc cgcaccggca gcaccgtcga 17400ggattgagct gacgccgggc tattttcaga taaccgccac gccgcatctt gccgtttatg 17460acccgacggt acagtttgag ttctggttct cggaaaagca gattgcggat atcagacagg 17520ttgaaaccag cacgcgttat cttggtacgg cgctgtactg gatagccgcc agtatcaata 17580tcaaaccggg ccatgattat tacttttata tccgcagtgt gaacaccgtt ggcaaatcgg 17640cattcgtgga ggccgtcggt cgggcgagcg atgatgcgga aggttacctg gattttttca 17700aaggcaagat aaccgaatcc catctcggca aggagctgct ggaaaaagtc gagctgacgg 17760aggataacgc cagcagactg gaggagtttt cgaaagagtg gaaggatgcc agtgataagt 17820ggaatgccat gtgggctgtc aaaattgagc agaccaaaga cggcaaacat tatgtcgcgg 17880gtattggcct cagcatggag gacacggagg aaggcaaact gagccagttt ctggttgccg 17940ccaatcgtat cgcatttatt gacccggcaa acgggaatga aacgccgatg tttgtggcgc 18000agggcaacca gatattcatg aacgacgtgt tcctgaagcg cctgacggcc cccaccatta 18060ccagcggcgg caatcctccg gccttttccc tgacaccgga cggaaagctg accgctaaaa 18120atgcggatat cagtggcagt gtgaatgcga actccgggac gctcagtaat gtgacgatag 18180ctgaaaactg tacgataaac ggtacgctga gggcggaaaa aatcgtcggg gacattgtaa 18240aggcggcgag cgcggctttt ccgcgccagc gtgaaagcag tgtggactgg ccgtcaggta 18300cccgtactgt caccgtgacc gatgaccatc cttttgatcg ccagatagtg gtgcttccgc 18360tgacgtttcg cggaagtaag cgtactgtca gcggcaggac aacgtattcg atgtgttatc 18420tgaaagtact gatgaacggt gcggtgattt atgatggcgc ggcgaacgag gcggtacagg 18480tgttctcccg tattgttgac atgccagcgg gtcggggaaa cgtgatcctg acgttcacgc 18540ttacgtccac acggcattcg gcagatattc cgccgtatac gtttgccagc gatgtgcagg 18600ttatggtgat taagaaacag gcgctgggca tcagcgtggt ctgagtgtgt tacagaggtt 18660cgtccgggaa cgggcgtttt attataaaac agtgagaggt gaacgatgcg taatgtgtgt 18720attgccgttg ctgtctttgc cgcacttgcg gtgacagtca ctccggcccg tgcggaaggt 18780ggacatggta cgtttacggt gggctatttt caagtgaaac cgggtacatt gccgtcgttg 18840tcgggcgggg ataccggtgt gagtcatctg aaagggatta acgtgaagta ccgttatgag 18900ctgacggaca gtgtgggggt gatggcttcc ctggggttcg ccgcgtcgaa aaagagcagc 18960acagtgatga ccggggagga tacgtttcac tatgagagcc tgcgtggacg ttatgtgagc 19020gtgatggccg gaccggtttt acaaatcagt aagcaggtca gtgcgtacct ggcacgacag 19080gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 19140ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 19200cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg 19260acactataga atactcaagc tatgcatcca acgcgttggg agctctccca tatggtcgac 19320ctgcaggcgg ccgcgaattc actagtgatt atggtaaagc aagatgaagt tatcacattg 19380ttatcaaata ttcgtagtaa tcgatgcaag atatattctt tgttaggaag ttcgcatgac 19440ttgagtgaaa atttagtggt cctgcgcaat ttttatcaat cggttacgaa agccgctatc 19500gcgatggata atgatcatct ggatattgat gttgatatta ctgaaccgtc atttgaacat 19560ttaactattg cgacagtcaa tgaacgccga atgagaattg agattgaaaa tcaagcaatt 19620tctctgtctt aaaatctatt gagatattct atcactcaaa tagcaatata aggactctct 19680atgaaatttg gaaacttttt gcttacatac caacctcccc aattttctca aacagaggta 19740atgaaacgtt tggttaaatt aggtcgcatc tctgaggagt gtggttttga taccgtatgg 19800ttactggagc atcatttcac ggagtttggt ttgcttggta acccttatgt cgctgctgca 19860tatttacttg gcgcgactaa aaaattgaat gtaggaactg ccgctattgt tcttcccaca 19920gcccatccag tacgccaact tgaagatgtg aatttattgg atcaaatgtc aaaaggacga 19980tttcggtttg gtatttgccg agggctttac aacaaggact ttcgcgtatt cggcacagat 20040atgaataaca gtcgcgcctt agcggaatgc tggtacgggc tgataaagaa tggcatgaca 20100gagggatata tggaagctga taatgaacat atcaagttcc ataaggtaaa agtaaacccc 20160gcggcgtata gcagaggtgg cgcaccggtt tatgtggtgg ctgaatcagc ttcgacgact 20220gagtgggctg ctcaatttgg cctaccgatg atattaagtt ggattataaa tactaacgaa 20280aagaaagcac aacttgagct ttataatgaa gtggctcaag aatatgggca cgatattcat 20340aatatcgacc attgcttatc atatataaca tctgtagatc atgactcaat taaagcgaaa 20400gagatttgcc ggaaatttct ggggcattgg tatgattctt atgtgaatgc tacgactatt 20460tttgatgatt cagaccaaac aagaggttat gatttcaata aagggcagtg gcgtgacttt 20520gtattaaaag gacataaaga tactaatcgc cgtattgatt acagttacga aatcaatccc 20580gtgggaacgc cgcaggaatg tattgacata attcaaaaag acattgatgc tacaggaata 20640tcaaatattt gttgtggatt tgaagctaat ggaacagtag acgaaattat tgcttccatg 20700aagctcttcc agtctgatgt catgccattt cttaaagaaa aacaacgttc gctattatat 20760tagctaagga gaaagaaatg aaatttggat tgttcttcct taacttcatc aattcaacaa 20820ctgttcaaga acaaagtata gttcgcatgc aggaaataac ggagtatgtt gataagttga 20880attttgaaca gattttagtg tatgaaaatc atttttcaga taatggtgtt gtcggcgctc 20940ctctgactgt ttctggtttt ctgctcggtt taacagagaa aattaaaatt ggttcattaa 21000atcacatcat tacaactcat catcctgtcg ccatagcgga ggaagcttgc ttattggatc 21060agttaagtga agggagattt attttagggt ttagtgattg cgaaaaaaaa gatgaaatgc 21120atttttttaa tcgcccggtt gaatatcaac agcaactatt tgaagagtgt tatgaaatca 21180ttaacgatgc tttaacaaca ggctattgta atccagataa cgatttttat agcttcccta 21240aaatatctgt aaatccccat gcttatacgc caggcggacc tcggaaatat gtaacagcaa 21300ccagtcatca tattgttgag tgggcggcca aaaaaggtat tcctctcatc tttaagtggg 21360atgattctaa tgatgttaga tatgaatatg ctgaaagata taaagccgtt gcggataaat 21420atgacgttga cctatcagag atagaccatc agttaatgat attagttaac tataacgaag 21480atagtaataa agctaaacaa gagacgcgtg catttattag tgattatgtt cttgaaatgc 21540accctaatga aaatttcgaa aataaacttg aagaaataat tgcagaaaac gctgtcggaa 21600attatacgga gtgtataact gcggctaagt tggcaattga aaagtgtggt gcgaaaagtg 21660tattgctgtc ctttgaacca atgaatgatt tgatgagcca aaaaaatgta atcaatattg 21720ttgatgataa tattaagaag taccacatgg aatataccta atagatttcg agttgcagcg 21780aggcggcaag tgaacgaatc cccaggagca tagataacta tgtgactggg gtgagtgaaa 21840gcagccaaca aagcagcagc ttgaaagatg aagggtataa aagagtatga cagcagtgct 21900gccatacttt ctaatattat cttgaggagt aaaacaggta tgacttcata tgttgataaa 21960caagaaatta cagcaagctc agaaattgat gatttgattt tttcgagcga tccattagtg 22020tggtcttacg acgagcagga aaaaatcaga aagaaacttg tgcttgatgc atttcgtaat 22080cattataaac attgtcgaga atatcgtcac tactgtcagg cacacaaagt agatgacaat 22140attacggaaa ttgatgacat acctgtattc ccaacatcgg tttttaagtt tactcgctta 22200ttaacttctc aggaaaacga gattgaaagt tggtttacca gtagcggcac gaatggttta 22260aaaagtcagg tggcgcgtga cagattaagt attgagagac tcttaggctc tgtgagttat 22320ggcatgaaat atgttggtag ttggtttgat catcaaatag aattagtcaa tttgggacca 22380gatagattta atgctcataa tatttggttt aaatatgtta tgagtttggt ggaattgtta 22440tatcctacga catttaccgt aacagaagaa cgaatagatt ttgttaaaac attgaatagt 22500cttgaacgaa taaaaaatca agggaaagat ctttgtctta ttggttcgcc atactttatt 22560tatttactct gccattatat gaaagataaa aaaatctcat tttctggaga taaaagcctt 22620tatatcataa ccggaggcgg ctggaaaagt tacgaaaaag aatctctgaa acgtgatgat 22680ttcaatcatc ttttatttga tactttcaat ctcagtgata ttagtcagat ccgagatata 22740tttaatcaag ttgaactcaa cacttgtttc tttgaggatg aaatgcagcg taaacatgtt 22800ccgccgtggg tatatgcgcg agcgcttgat cctgaaacgt tgaaacctgt acctgatgga 22860acgccggggt tgatgagtta tatggatgcg tcagcaacca gttatccagc atttattgtt 22920accgatgatg tcgggataat tagcagagaa tatggtaagt atcccggcgt gctcgttgaa 22980attttacgtc gcgtcaatac gaggacgcag aaagggtgtg ctttaagctt aaaccaagca 23040tttaatagtt gacataatcg aattcccgcg gccgccatgg cggccgggag catgcgacgt 23100cgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg 23160tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 23220cgccaggtac gccatggccg gagtggctca cagtcggtgg tccggcagta caatggatta 23280ccgtaagacg gaaatcactc ccgggatcct ctagagtcga cctgcaggca tgcaagcttg 23340gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 23400aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 23460acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 23520cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 23580tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 23640tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 23700gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 23760aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 23820ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 23880gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 23940ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 24000ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 24060cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 24120attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 24180ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 24240aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 24300gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 24360tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 24420ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 24480taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 24540atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 24600actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 24660cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 24720agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 24780gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 24840gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 24900gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 24960gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 25020cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 25080ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 25140accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga

25200aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 25260aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 25320caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 25380ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 25440gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 25500cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 25560aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 25620ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 25680gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 25740gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 25800cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 25860gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 25920gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgatttt 25980aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 26040ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 26100taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 26160ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt tatctagagt 26220cgcggccgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga 26280actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg 26340tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct 26400ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca 26460ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt 26520actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga 26580tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt 26640cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga 26700agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg 26760tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg 26820tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt 26880ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct 26940tgctcaccat ggtggcgacc ggtggatcga tcctagcgga tctgacggtt cactaaacca 27000gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 27060gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 27120acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 27180atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 27240caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 27300tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 27360gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 27420atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 27480gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 27540cccgtaattg attactatta aatcactagt gaattcgatt aaagcgacgg cacagctcgc 27600ggaaaatatc aaagtcgttg cgcgcctcga actgcggcgg caccacctgt ttcatggcga 27660taatgccacg gttggagtgg ttgccgtact ggtcgagatc gttacgctca aactgcgtgg 27720tcgcaggcag cacgatatcg gcaaagcggc aggttgaggt ccactggtta tctatggcga 27780taaccgtttc cagcttgcgc cagccttcaa taatgcggtt gatctgctga tggcgatgga 27840atgggttagt tccggcaaaa atacacattt tcagcggcgg cagttttacc gatttaccgt 27900tccagttgat cactttcccc ggttcgagga tcgcatcgat aaaacgggca atcggaatgg 27960tgctgctgta gcctttgtaa tcactgttgt cgtgaacagg cggaatcgac gtagagccgg 28020agaaaccact cagaataacg cctttacgcc ccggcgtgcc tgcgccgtta tagtgccagc 28080caaaaccaaa gccaccacct ggcaggccaa tttgccccag catcgccgcc agaaccacaa 28140tcatccacgc ccactgttca ccgtgctgca tacgctgcac gcaccagcca gcaataattt 28200gcgttctgtt cgccgccatc tgccgcgcca gcccacgaat ggtttcggca tcaatgccgg 28260tcagtttttc agcccatgcg gcatctttcg gctgaccgtc tttctcaccc agcaggtacg 28320gcaggaactg ctcaaaaccc acacagtagt tagcgaggaa gtttttgtcg tacaggtttt 28380cactgtacag cgtatacgcc agcgccagtt gcagcggcac atcagtttgc gggttaaccg 28440caatgtgctt cacatgctcg cgccccagat actcatgggt ggatgtgaca accggatcga 28500tgctgatgac ctcaatttca ccggcggtga ctttcgcttt tagctgcgcg taatattcat 28560aaacatcgtg atccgggcac caccagttcg cttgctggtt tttcagcaaa tcagagcccc 28620acagcacaat ggttttgctg ttctgcaata ccagcggcca ggaggtttgc tgttcataca 28680cttccattga gccaaccacg cgcggcagga tcacctgcgc agcaccggta gagtaatctc 28740cgcccgtacc aacgctatta ccatgcaagg caatagcttt cgccagcatc cccgaagcgt 28800tatggaacat ccccgtcgat tgccaaccac tggcggtcag caaggcactc ggcccgtgag 28860ttttctgcac gcgttccagt tcttcataga acatgtcgag ggcttcatcc cagctcacgc 28920gcacaaaacg gttatcaccg cgctgggagg tatcgctgag atggcgctta cgcagccagt 28980ccacgcgtac catcggataa cgaatacgcg ccgcgttgtg tacgtgatcc ggcaatccgg 29040caatcatttt cgacggatat ttatccagtt cgaacggttt tgccgccaca aagcgaccat 29100ccttcaccgt cgcgcggata gccccccagt gcgacccggt aagaatgccc tctttcgaga 29160tgacagcctc agtcgccgct tgcgccgcag tcgcacggcg cggcgttaac aatgacggcc 29220ccagcatccc ggcgacggtt aagccgccga gttgtgccag aaaacgccga cgtgatgcct 29280gaaagagatc gttattgttc attatttttc ttccttctta tcgccgtgag ccttacctgc 29340ggtgtcagac gcattcattt gcagatattt caacaaagtg cgttcttcac gtttatcgag 29400actggtaaag ccaatcatgc cgttgagcgt gccgatccaa ccgttagcgt caaagtgggc 29460gatttccggt gcgccgtggc actggttaca ggtgccgttg tacaacgaat ccgcataagc 29520ccagatcggt ttgatatcgt tcaccatgtc gcctttcttc atccacgcag tggcctgcaa 29580cttgctccac tcggtattgg tgtcggcaac ggtggttttc tccagcgttt ttacctgctg 29640ctgcacatca ccacgaatcg aggcaacaaa gatgcgttta cctgggaatt gggtgagtac 29700acgctgacgt ccggcgcttt ccgtccagcc ggtaatttca atttgcagcc agtcgccgtc 29760acgtttaagg actttcactt ccgaagcagg cagcagagaa ccagaggctt ctttatcgcc 29820tttcgccgca taaattggct taatatcaat agagtacagc gtgtcaccac tgtcattagc 29880actggcgcgc agctcatcga actgcttacg gaagccgcta ctcatatccg gtaactggtg 29940ggcaatacct ttatgacagt cgatgcagga ttgattatct ttcgctgcca ccttcatctg 30000acgtgccgct tcaggatgct gcttcgcatg atccatcgca tcgtagttat ggcaggagcg 30060gcaggttgcc gagttgtttt ctttcattcg cgcccattca cgctcggcaa gttccgcgcg 30120tttggcttcg aatttttcag gtgtatcaat ggagtgagca ataaaggtct ggtagatatc 30180attgctcgct tccagtttgc gcttcaccat gcctggaata tccggcggga tatgacagtc 30240atggcattca gctcgcacgc cggaggcgtt ctggaaatgc accgactgtt tatattcttc 30300atacaccggt tgcatactgt ggcaactgac acaaaattcg gttgtgctgg tgactttgat 30360cccaacgtgt ggcaatacaa tcagcgcaat gccaatcaca atcccaattg cgaccagcgc 30420cagtaccgac caacgagcac tgggtcggcg tagcgcgttc cagagtttcc gcataatagc 30480ccctgtaaaa ttatggttta gtgaagcgat cttaatgagc aaatatgaac agcggcactg 30540gtcaggatga acggcttacg gcagaatatg aacagatatg aacagaatga gtaaaaccct 30600ctgatgccac atcacattgt tattgttgaa gatgagccgg ttacccaggc gcgattacaa 30660tcttacttca ctcaggaggg gtataccgtt tccgttacag cgagcggtgc cgggctgcgg 30720gaaattatgc agaatcagcc ggtagattta attctgctgg atatcaactt acccgatgaa 30780aatggcctga tgttaacccg cgccctgcga gaacgctcaa cggtggggat tattctggtt 30840accggacgca gcgatcggat tgaccgtatt gttgggctgg aaatgggcgc agacgattac 30900gtcaccaaac cgctggaact gcgcgaactg gtagtacggg tgaaaaatct gctctggcga 30960atcgacctcg cgcgacaagc tcaaccgcac actcaggaca actgctatcg ctttgccggt 31020tattgcctga atgtgtcgcg ccatacgctg gagcgggatg gcgagccgat taaactgacc 31080cgcgcagagt atgaaatgtt ggtggcattt gtgacgaatc cgggcgaaat tctcagccgt 31140gaacgtctgc tacgtatgct ttctgcgcgt cgggtggaaa accctgacct gcgcaccgtc 31200gatgtgttaa ttcgtcgttt acgtcataaa ctcagcgcgg atttactggt gacgcaacat 31260ggtgaaggtt atttcttagc cgctgatgtg tgctgataaa aatagaccgg acgaaatccc 31320cctggtgaca gcgagcggcg gatatgttct cggtcggcat ttttcggcgt cagaactaaa 31380atcggtgggc tgacattatc agacaccgat tgcccctgta attgcctgat ggcctgctca 31440actgccagtt ccccctgcca gaccatttga tcgctggcag ccataatcac tcttccccgc 31500ttcagcccgc gatacacctg atgtgaaaga taaaacgaca ccacggtaag cggcgttttc 31560aggttacgcc cttcacccat tgccgcctct gccgcaatgg ccgttccggc aacgacgtca 31620atttctgggt ggcgttccag catctcctgc aacaggttac gctggatttc aatatcgtta 31680tcaccaagcg caatatcaac aatacgcacc gggcttccgg caatggctgc gcgaaaaccc 31740tcgaccatct ctttactgcc cccggcatta tcgggtccgg gcatcaacag cacgttcagt 31800ggtttaccgt gcgcccattg caccaaatat cgcccaggtt gatagcccat ctgaaaccag 31860ggtacaccaa cgcggctttt cacctgggga gcatcaatag catttaccag ttcgatcacc 31920ggcagacttg ctacctgctt ttgcagatcg ggaaatgagg tcgtgctact accgagtaaa 31980atggcctctg cgccccactg tttacactgg tcgatttgtg cttgctgggt agccaactgg 32040ctgtagccgc ctgcctccag cacttttaaa tccacaccat agcggcgagc tgcctcctgc 32100ataccatagt tcaacgataa ccagtatgaa tctttcaggc tgggataaag cgcgcacagt 32160ttccatgcgc gtttggcttt aagcggcata gaggcttgca ccgtgaaatg ctgcgcatca 32220tgccagcgca acaggttatc agccgaaaat gccggcaaca tgaaaaggga aagaagtaaa 32280aatagcagta cgcgcatgat agcctcatca ataataaggc tttatgctag atgcattccg 32340ctttgcgact caaccttttt caccttaagt gcaccgaccg tgaatttaac cctgacccga 32400agactctgga tgggctttgc cctgatggcg ctgttaaccc tgaccagtac cctggtggga 32460tggtacaacc tgcgctttat cagccaggtg gaaaaagaca acactcaggc attgattcct 32520accatgaata tggcgcgcca gttgagcgaa gccagcgcct gggaactttt cgccgcgcag 32580aacctgacca gtgccgataa cgaaaagatg tggcaggcgc aggggcgaat gctcaccgca 32640caaagcctga agattaatgc gttgctgcaa gcgttacggg aacaaggttt tgataccacc 32700gctattgaac aacaggagca ggagatctcc cgttcattac gtcagcaagg ggaactggtg 32760gggcggcgtc tgcaactacg ccagcaacaa cggcaactca gtcagcagat agtcgctgcc 32820gccgatgaga tcgcacgcct ggcgcaaggt caggcgaata atgcgacaac ttccgctgga 32880gcgacccagg ccgggattta cgatttgatc gaacaagatc agcgtcaggc tgctgaaagt 32940gcactcgatc ggctgattga tatcgatctt gagtatgtta accagatgaa tgaactgcgc 33000cttagcgctc tgcgggtgca gcaaatggtg atgaatctgg ggctggagca gatccagaaa 33060aatgcaccaa cgctggaaaa gcagctcaat aatgcggtga aaattctgca acgtcggcaa 33120atacgcattg aagatccggg tgttcgtgcg caggtcgcaa caacgttaac taccgttagc 33180caatatagcg atttgctggc gctgtatcag caggacagtg aaatcagcaa tcacctacaa 33240actctcgcac aaaataacat cgcccagttc gcgcagttta gtagcgaagt cagtcagctg 33300gtcgactcgg tacccgggga tccactcgtt attctcggac gagtgttcag taatgaacct 33360ctggagagaa ccatgtatat gatcgttatc tgggttggac ttctgctttt aagcccagat 33420aactggcctg aatatgttaa tgagagaatc ggtattcctc atgtgtggca tgttttcgtc 33480tttgctcttg cattttcgct agcaattaat gtgcatcgat tatcagctat tgccagcgcc 33540agatataagc gatttaagct aagaaaacgc attaagatgc aaaacgataa agtgcgatca 33600gtaattcaaa accttacaga agagcaatct atggttttgt gcgcagccct taatgaaggc 33660aggaagtatg tggttacatc aaaacaattc ccatacatta gtgagttgat tgagcttggt 33720gtgttgaaca aaactttttc ccgatggaat ggaaagcata tattattccc tattgaggat 33780atttactgga ctgaattagt tgccagctat gatccatata atattgagat aaagccaagg 33840ccaatatcta agtaactaga taagaggaat cgattttccc ttaattttct ggcgtccact 33900gcatgttatg ccgcgttcgc caggcttgct gtaccatgtg cgctgattct tgcgctcaat 33960acgttgcagg ttgctttcaa tctgtttgtg gtattcagcc agcactgtaa ggtctatcgg 34020atttagtgcg ctttctactc gtgatttcgg tttgcgattc agcgagagaa tagggcggtt 34080aactggtttt gcgcttaccc caaccaacag gggatttgct gctttccatt gagcctgttt 34140ctctgcgcga cgttcgcggc ggcgtgtttg tgcatccatc tggattctcc tgtcagttag 34200ctttggtggt gtgtggcagt tgtagtcctg aacgaaaacc ccccgcgatt ggcacattgg 34260cagctaatcc ggaatcgcac ttacggccaa tgcttcgttt cgtatcacac accccaaagc 34320cttctgcttt gaatgctgcc cttcttcagg gcttaatttt taagagcgtc accttcatgg 34380tggtcagtgc gtcctgctga tgtgctcagt atcaccgcca gtggtattta tgtcaacacc 34440gccagagata atttatcacc gcagatggtt atctgtatgt tttttatatg aatttatttt 34500ttgcaggggg gcattgtttg gtaggtgaga gatctgaatt gctatgttta gtgagttgta 34560tctatttatt tttcaataaa tacaattggt tatgtgtttt gggggcgatc gtgaggcaaa 34620gaaaacccgg cgctgaggcc gggttattct tgttctctgg tcaaattata tagttggaaa 34680acaaggatgc atatatgaat gaacgatgca gaggcaatgc cgatggcgat agtgggtatc 34740atgtagccgc ttatgctgga aagaagcaat aacccgcaga aaaacaaagc tccaagctca 34800acaaaactaa gggcatagac aataactacc gatgtcatat acccatactc tctaatcttg 34860gccagtcggc gcgttctgct tccgattaga aacgtcaagg cagcaatcag gattgcaatc 34920atggttcctg catatgatga caatgtcgcc ccaagaccat ctctatgagc tgaaaaagaa 34980acaccaggaa tgtagtggcg gaaaaggaga tagcaaatgc ttacgataac gtaaggaatt 35040attactatgt aaacaccagg catgattctg ttccgcataa ttactcctga taattaatcc 35100ttaactttgc ccacctgcct tttaaaacat tccagtatat cacttttcat tcttgcgtag 35160caatatgcca tctcttcagc tatctcagca ttggtgacct tgttcagagg cgctgagaga 35220tggccttttt ctgatagata atgttctgtt aaaatatctc cggcctcatc ttttgcccgc 35280aggctaatgt ctgaaaattg aggtgacggg ttaaaaataa tatccttggc aacctttttt 35340atatcccttt taaattttgg cttaatgact atatccaatg agtcaaaaag ctccccttca 35400atatctgttg cccctaagac ctttaatata tcgccaaata caggtagctt ggcttctacc 35460ttcaccgttg ttcggccgat gaaatgcata tgcataacat cgtctttggt ggttcccctc 35520atcagtggct ctatctgaac gcgctctcca ctgcttaatg acattccttt cccgattaaa 35580aaatctgtca gatcggatgt ggtcggcccg aaaacagttc tggcaaaacc aatggtgtcg 35640ccttcaacaa acaaaaaaga tgggaatccc aatgattcgt catctgcgag gctgttctta 35700atatcttcaa ctgaagcttt agagcgattt atcttctgaa ccagactctt gtcatttgtt 35760ttggtaaaga gaaaagtttt tccatcgatt ttatgaatat acaaataatt ggagccaacc 35820tgcaggtgat gattatcagc cagcagagaa ttaaggaaaa cagacaggtt tattgagcgc 35880ttatctttcc ctttattttt gctgcggtaa gtcgcataaa aaccattctt cataattcaa 35940tccatttact atgttatgtt ctgaggggag tgaaaattcc cctaattcga tgaagattct 36000tgctcaattg ttatcagcta tgcgccgacc agaacacctt gccgatcagc caaacgtctc 36060ttcaggccac tgactagcga taactttccc cacaacggaa caactctcat tgcatgggat 36120cattgggtac tgtgggttta gtggttgtaa aaacacctga ccgctatccc tgatcagttt 36180cttgaaggta aactcatcac ccccaagtct ggctatgcag aaatcacctg gctcaacagc 36240ctgctcaggg tcaacgagaa ttaacattcc gtcaggaaag cttggcttgg agcctgttgg 36300tgcggtcatg gaattacctt caacctcaag ccagaatgca gaatcactgg cttttttggt 36360tgtgcttacc catctctccg catcaccttt ggtaaaggtt ctaagctcag gtgagaacat 36420ccctgcctga acatgagaaa aaacagggta ctcatactca cttctaagtg acggctgcat 36480actaaccgct tcatacatct cgtagatttc tctggcgatt gaagggctaa attcttcaac 36540gctaactttg agaatttttg caagcaatgc ggcgttataa gcatttaatg cattgatgcc 36600attaaataaa gcaccaacgc ctgactgccc catccccatc ttgtctgcga cagattcctg 36660ggataagcca agttcatttt tctttttttc ataaattgct ttaaggcgac gtgcgtcctc 36720aagctgctct tgtgttaatg gtttcttttt tgtgctcata cgttaaatct atcaccgcaa 36780gggataaata tctaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg 36840catgtactaa ggaggttgta tggaacaacg cataaccctg aaagattatg caatgcgctt 36900tgggcaaacc aagacagcta aagatctcgg cgtatatcaa agcgcgatca acaaggccat 36960tcatgcaggc cgaaagattt ttttaactat aaacgctgat ggaagcgttt atgcggaaga 37020ggtaaagccc ttcccgagta acaaaaaaac aacagcataa ataaccccgc tcttacacat 37080tccagccctg aaaaagggca tcaaattaaa ccacacctat ggtgtatgca tttatttgca 37140tacattcaat caattgttat ctaaggaaat acttacatat ggttcgtgca aacaaacgca 37200acgaggctct acgaatcgag agtgcgttgc ttaacaaaat cgcaatgctt ggaactgaga 37260agacagcgga agctgtgggc gttgataagt cgcagatcag caggtggaag agggactgga 37320ttccaaagtt ctcaatgctg cttgctgttc ttgaatgggg ggtcgttgac gacgacatgg 37380ctcgattggc gcgacaagtt gctgcgattc tcaccaataa aaaacgcccg gcggcaaccg 37440agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 37500cattatgaca aatacagcaa aaatactcaa cttcggcaga ggtaactttg ccggacagga 37560gcgtaatgtg gcagatctcg atgatggtta cgccagacta tcaaatatgc tgcttgaggc 37620ttattcgggc gcagatctga ccaagcgaca gtttaaagtg ctgcttgcca ttctgcgtaa 37680aacctatggg tggaataaac caatggacag aatcaccgat tctcaactta gcgagattac 37740aaagttacct gtcaaacggt gcaatgaagc caagttagaa ctcgtcagaa tgaatattat 37800caagcagcaa ggcggcatgt ttggaccaaa taaaaacatc tcagaatggt gcatccctca 37860aaacgaggga aaatccccta aaacgaggga taaaacatcc ctcaaattgg gggattgcta 37920tccctcaaaa cagggggaca caaaagacac tattacaaaa gaaaaaagaa aagattattc 37980gtcagannnn nntggcgaat cctctgacca gccagaaaac gacctttctg tggtgaaacc 38040ggatgctgca attcagagcg gcagcaagtg ggggacagca gaagacctga ccgccgcaga 38100gtggatgttt gacatggtga agactatcgc accatcagcc agaaaaccga attttgctgg 38160gtgggctaac gatatccgcc tgatgcgtga acgtgacgga cgtaaccacc gcgacatgtg 38220tgtgctgttc cgctgggcat gccaggacaa cttctggtcc ggtaacgtgc tgagcccggc 38280caaactccgc gataagtgga cccaactcga aatcaaccgt aacaagcaac aggcaggcgt 38340gacagccagc aaaccaaaac tcgacctgac aaacacagac tggatttacg gggtggatct 38400atgaaaaaca tcgccgcaca gatggttaac tttgaccgtg agcagatgcg tcggatcgcc 38460aacaacatgc cggaacagta cgacgaaaag ccgcaggtac agcaggtagc gcagatcatc 38520aacggtgtgt tcagccagtt actggcaact ttcccggcga gcctggctaa ccgtgaccag 38580aacgaagtga acgaaatccg tcgccagtgg gttctggctt ttcgggaaaa cgggatcacc 38640acgatggaac aggttaacgc aggaatgcgc gtagcccgtc ggcagaatcg accatttctg 38700ccatcacccg ggcagtttgt tgcatggtgc cgggaagaag catccgttac cgccggactg 38760ccaaacgtca gcgagctggt tgatatggtt tacgagtatt gccggaagcg aggcctgtat 38820ccggatgcgg agtcttatcc gtggaaatca aacgcgcact actggctggt taccaacctg 38880tatcagaaca tgcgggccaa tgcgcttact gatgcggaat tacgccgtaa ggccgcagat 38940gagcttgtcc atatgactgc gagaattaac cgtggtgagg cgatccctga accagtaaaa 39000caacttcctg tcatgggcgg tagacctcta aatcgtgcac aggctctggc gaagatcgca 39060gaaatcaaag ctaagttcgg actgaaagga gcaagtgtat gacgggcaaa gaggcaatta 39120ttcattacct ggggacgcat aatagcttct gtgcgccgga cgttgccgcg ctaacaggcg 39180caacagtaac cagcataaat caggccgcgg ctaaaatggc acgggcaggt cttctggtta 39240tcgaaggtaa ggtctggcga acggtgtatt accggtttgc taccagggaa gaacgggaag 39300gaaagatgag cgatgaacaa actggatacg attggattcg acaacaaaaa agacctgctt 39360atctcggtgg gcgatttggt tgatcgtggt gcagagaacg ttgaatgcct ggaattaatc 39420acattcccct ggttcagagc tgtacgtgga aaccatgagc aaatgatgat tgatggctta 39480tcagagcgtg gaaacgttaa tcactggctg cttaatggcg gtggctggtt ctttaatctc 39540gattacgaca aagaaattct ggctaaagct cttgcccata aagcagatga acttccgtta 39600atcatcgaac tggtgagcaa agataaaaaa tatgttatct gccacgccga ttatcccttt 39660gacgaatacg agtttggaaa gccagttgat catcagcagg taatctggaa ccgcgaacga 39720atcagcaact cacaaaacgg gatcgtgaaa gaaatcaaag gcgcggacac gttcatcttt 39780ggtcatacgc cagcagtgaa accactcaag tttgccaacc aaatgtatat cgataccggc 39840gcagtgttct gcggaaacct aacattgatt caggtacagg gagaaggcgc atgagactcg 39900aaagcgtagc taaatttcat tcgccaaaaa gcccgatgat gagcgactca ccacgggcca 39960cggcttctga ctctctttcc ggtactgatg tgatggctgc tatggggatg gcgcaatcac 40020aagccggatt cggtatggct gcattctgcg gtaagcacga actcagccag aacgacaaac 40080aaaaggctat caactatctg atgcaatttg cacacaaggt atcggggaaa taccgtggtg 40140tggcaaagct tgaaggaaat actaaggcaa aggtactgca agtgctcgca acattcgctt 40200atgcggatta ttgccgtagt gccgcgacgc cgggggcaag atgcagagat tgccatggta

40260caggccgtgc ggttgatatt gccaaaacag agctgtgggg gagagttgtc gagaaagagt 40320gcggaagatg caaaggcgtc ggctattcaa ggatgccagc aagcgcagca tatcgcgctg 40380tgacgatgct aatcccaaac cttacccaac ccacctggtc acgcactgtt aagccgctgt 40440atgacgctct ggtggtgcaa tgccacaaag aagagtcaat cgcagacaac attttgaatg 40500cggtcacacg ttagcagcat gattgccacg gatggcaaca tattaacggc atgatattga 40560cttattgaat aaaattgggt aaatttgact caacgatggg ttaattcgct cgttgtggta 40620gtgagatgaa aagaggcggc gcttactacc gattccgcct agttggtcac ttcgacgtat 40680cgtctggaac tccaaccatc gcaggcagag aggtctgcaa aatgcaatcc cgaaacagtt 40740cgcaggtaat agttagagcc tgcataacgg tttcgggatt ttttatatct gcacaacagg 40800taagagcatt gagtcgataa tcgtgaagag tcggcgagcc tggttagcca gtgctctttc 40860cgttgtgctg aattaagcga ataccggaag cagaaccgga tcaccaaatg cgtacaggcg 40920tcatcgccgc ccagcaacag cacaacccaa actgagccgt agccactgtc tgtcctnnnn 40980nnattagtaa tagttacgct gcggcctttt acacatgacc ttcgtgaaag cgggtggcag 41040gaggtcgcgc taacaacctc ctgccgtttt gcccgtgcat atcggtcacg aacaaatctg 41100attactaaac acagtagcct ggatttgttc tatcagtaat cgaccttatt cctaattaaa 41160tagagcaaat ccccttattg ggggtaagac atgaagatgc cagaaaaaca tgacctgttg 41220gccgccattc tcgcggcaaa ggaacaaggc atcggggcaa tccttgcgtt tgcaatggcg 41280taccttcgcg gcagatataa tggcggtgcg tttacaaaaa cagtaatcga cgcaacgatg 41340tgcgccatta tcgcctggtt cattcgtgac cttctcgact tcgccggact aagtagcaat 41400ctcgcttata taacgagcgt gtttatcggc tacatcggta ctgactcgat tggttcgctt 41460atcaaacgct tcgctgctaa aaaagccgga gtagaagatg gtagaaatca ataatcaacg 41520taaggcgttc ctcgatatgc tggcgtggtc ggagggaact gataacggac gtcagaaaac 41580cagaaatcat ggttatgacg tcattgtagg cggagagcta tttactgatt actccgatca 41640ccctcgcaaa cttgtcacgc taaacccaaa actcaaatca acaggcgccg gacgctacca 41700gcttctttcc cgttggtggg atgcctaccg caagcagctt ggcctgaaag acttctctcc 41760gaaaagtcag gacgctgtgg cattgcagca gattaaggag cgtggcgctt tacctatgat 41820tgatcgtggt gatatccgtc aggcaatcga ccgttgcagc aatatctggg cttcactgcc 41880gggcgctggt tatggtcagt tcgagcataa ggctgacagc ctgattgcaa aattcaaaga 41940agcgggcgga acggtcagag agattgatgt atgagcagag tcaccgcgat tatctccgct 42000ctggttatct gcatcatcgt ctgcctgtca tgggctgtta atcattaccg tgataacgcc 42060attacctaca aagcccagcg cgacaaaaat gccagagaac tgaagctggc gaacgcggca 42120attactgaca tgcagatgcg tcagcgtgat gttgctgcgc tcgatgcaaa atacacgaag 42180gagttagctg atgctaaagc tgaaaatgat gctctgcgtg atgatgttgc cgctggtcgt 42240cgtcggttgc acatcaaagc agtctgtcag tcagtgcgtg aagccaccac cgcctccggc 42300gtggataatg cagcctcccc ccgactggca gacaccgctg aacgggatta tttcaccctc 42360agagagaggc tgatcactat gcaaaaacaa ctggaaggaa cccagaagta tattaatgag 42420cagtgcagat agagttgccc atatcgatgg gcaactcatg caattattgt gagcaataca 42480cacgcgcttc cagcggagta taaatgccta aagtaataaa accgagcaat ccatttacga 42540atgtttgctg ggtttctgtt ttaacaacat tttctgcgcc gccacaaatt ttggctgcat 42600cgacagtttt cttctgccca attccagaaa cgaagaaatg atgggtgatg gtttcctttg 42660gtgctactgc tgccggtttg ttttgaacag taaacgtctg ttgagcacat cctgtaataa 42720gcagggccag cgcagtagcg agtagcattt ttttcatggt gttattcccg atgctttttg 42780aagttcgcag aatcgtatgt gtagaaaatt aaacaaaccc taaacaatga gttgaaattt 42840catattgtta atatttatta atgtatgtca ggtgcgatga atcgtcattg tattcccgga 42900ttaactatgt ccacagccct gacggggaac ttctctgcgg gagtgtccgg gaataattaa 42960aacgatgcac acagggttta gcgcgtacac gtattgcatt atgccaacgc cccggtgctg 43020acacggaaga aaccggacgt tatgatttag cgtggaaaga tttgtgtagt gttctgaatg 43080ctctcagtaa atagtaatga attatcaaag gtatagtaat atcttttatg ttcatggata 43140tttgtaaccc atcggaaaac tcctgcttta gcaagatttt ccctgtattg ctgaaatgtg 43200atttctcttg atttcaacct atcataggac gtttctataa gatgcgtgtt tcttgagaat 43260ttaacattta caaccttttt aagtcctttt attaacacgg tgttatcgtt ttctaacacg 43320atgtgaatat tatctgtggc tagatagtaa atataatgtg agacgttgtg acgttttagt 43380tcagaataaa acaattcaca gtctaaatct tttcgcactt gatcgaatat ttctttaaaa 43440atggcaacct gagccattgg taaaaccttc catgtgatac gagggcgcgt agtttgcatt 43500atcgttttta tcgtttcaat ctggtctgac ctccttgtgt tttgttgatg atttatgtca 43560aatattagga atgttttcac ttaatagtat tggttgcgta acaaagtgcg gtcctgctgg 43620cattctggag ggaaatacaa ccgacagatg tatgtaaggc caacgtgctc aaatcttcat 43680acagaaagat ttgaagtaat attttaaccg ctagatgaag agcaagcgca tggagcgaca 43740aaatgaataa agaacaatct gctgatgatc cctccgtgga tctgattcgt gtaaaaaata 43800tgcttaatag caccatttct atgagttacc ctgatgttgt aattgcatgt atagaacata 43860aggtgtctct ggaagcattc agagcaattg aggcagcgtt ggtgaagcac gataataata 43920tgaaggatta ttccctggtg gttgactgat caccataact gctaatcatt caaactattt 43980agtctgtgac agagccaaca cgcagtctgt cactgtcagg aaagtggtaa aactgcaact 44040caattactgc aatgccctcg taattaagtg aatttacaat atcgtcctgt tcggagggaa 44100gaacgcggga tgttcattct tcatcacttt taattgatgt atatgctctc ttttctgacg 44160ttagtctccg acggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgct 44220caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg 44280ttctgcgggt tctgttcttc gttgacatga ggttgccccg tattcagtgt cgctgatttg 44340tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt 44400cataattgat tatttgacgt ggtttgatgg cctccacgca cgttgtgata tgtagatgat 44460aatcattatc actttacggg tcctttccgg tgatccgaca ggttacg 44507451940DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 4ctgtctctta tacacatctc aaccctgaag ctcttgttgg ctagtgcgta gtcgttggca 60agcttgcatg cctgcagtag ggataacagg gtaatgtcga cttgcatgcc tgcataaatt 120tgaattttat aaaaaattat gttataattc gcgcagtacg atggataatg atcatctgga 180tattgatgtt gatattactg aaccgtcatt tgaacattta actattgcga cagtcaatga 240acgccgaatg agaattgaga ttgaaaatca agcaatttct ctgtcttaaa atctattgag 300atattctatc actcaaatag caatataagg actctctatg aaatttggaa actttttgct 360tacataccaa cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg 420tcgcatctct gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga 480gtttggtttg cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa 540attgaatgta ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga 600agatgtgaat ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg 660gctttacaac aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc 720ggaatgctgg tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa 780tgaacatatc aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc 840accggtttat gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct 900accgatgata ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta 960taatgaagtg gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata 1020tataacatct gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg 1080gcattggtat gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag 1140aggttatgat ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac 1200taatcgccgt attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat 1260tgacataatt caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga 1320agctaatgga acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat 1380gccatttctt aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa 1440tttggattgt tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt 1500cgcatgcagg aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat 1560gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg 1620ctcggtttaa cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat 1680cctgtcgcca tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt 1740ttagggttta gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa 1800tatcaacagc aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc 1860tattgtaatc cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct 1920tatacgccag gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg 1980gcggccaaaa aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat 2040gaatatgctg aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata 2100gaccatcagt taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag 2160acgcgtgcat ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat 2220aaacttgaag aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg 2280gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg 2340aatgatttga tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac 2400cacatggaat atacctaata gatttcgagt tgcagcgagg cggcaagtga acgaatcccc 2460aggagcatag ataactatgt gactggggtg agtgaaagca gccaacaaag cagcagcttg 2520aaagatgaag ggtataaaag agtatgacag cagtgctgcc atactttcta atattatctt 2580gaggagtaaa acaggtatga cttcatatgt tgataaacaa gaaattacag caagctcaga 2640aattgatgat ttgatttttt cgagcgatcc atagatctgg agctaaggaa gctaaaatgg 2700agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt 2760ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta 2820cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca 2880ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc 2940tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt 3000tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc 3060aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata 3120tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca 3180atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca 3240aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg 3300gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttc 3360aattcattac cctgttatcc ctacccgaga aaattcatcg atgatggttg agatgtgtat 3420aagagacagg ttaaatctat caccgcaagg gataaatatc taacaccgtg cgtgttgact 3480attttacctc tggcggtgat aatggttgca tgtactaagg aggttgtatg gaacaacgca 3540taaccctgaa agattatgca atgcgctttg ggcaaaccaa gacagctaaa gatctcggcg 3600tatatcaaag cgcgatcaac aaggccattc atgcaggccg aaagattttt ttaactataa 3660acgctgatgg aagcgtttat gcggaagagg taaagccctt cccgagtaac aaaaaaacaa 3720cagcataaat aaccccgctc ttacacattc cagccctgaa aaagggcatc aaattaaacc 3780acacctatgg tgtatgcatt tatttgcata cattcaatca attgttatct aaggaaatac 3840ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac gaatcgagag tgcgttgctt 3900aacaaaatcg caatgcttgg aactgagaag acagcggaag ctgtgggcgt tgataagtcg 3960cagatcagca ggtggaagag ggactggatt ccaaagttct caatgctgct tgctgttctt 4020gaatgggggg tcgttgacga cgacatggct cgattggcgc gacaagttgc tgcgattctc 4080accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga 4140ggtcattact ggatctatca acaggagtca ttatgacaaa tacagcaaaa atactcaact 4200tcggcagagg taactttgcc ggacaggagc gtaatgtggc agatctcgat gatggttacg 4260ccagactatc aaatatgctg cttgaggctt attcgggcgc agatctgacc aagcgacagt 4320ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca atggacagaa 4380tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc aatgaagcca 4440agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt ggaccaaata 4500aaaacatctc agaatggtgc atccctcaaa acgagggaaa atcccctaaa acgagggata 4560aaacatccct caaattgggg gattgctatc cctcaaaaca gggggacaca aaagacacta 4620ttacaaaaga aaaaagaaaa gattattcgt cagagaattc tggcgaatcc tctgaccagc 4680cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat tcagagcggc agcaagtggg 4740ggacagcaga agacctgacc gccgcagagt ggatgtttga catggtgaag actatcgcac 4800catcagccag aaaaccgaat tttgctgggt gggctaacga tatccgcctg atgcgtgaac 4860gtgacggacg taaccaccgc gacatgtgtg tgctgttccg ctgggcatgc caggacaact 4920tctggtccgg taacgtgctg agcccggcca aactccgcga taagtggacc caactcgaaa 4980tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa accaaaactc gacctgacaa 5040acacagactg gatttacggg gtggatctat gaaaaacatc gccgcacaga tggttaactt 5100tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg gaacagtacg acgaaaagcc 5160gcaggtacag caggtagcgc agatcatcaa cggtgtgttc agccagttac tggcaacttt 5220cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac gaaatccgtc gccagtgggt 5280tctggctttt cgggaaaacg ggatcaccac gatggaacag gttaacgcag gaatgcgcgt 5340agcccgtcgg cagaatcgac catttctgcc atcacccggg cagtttgttg catggtgccg 5400ggaagaagca tccgttaccg ccggactgcc aaacgtcagc gagctggttg atatggttta 5460cgagtattgc cggaagcgag gcctgtatcc ggatgcggag tcttatccgt ggaaatcaaa 5520cgcgcactac tggctggtta ccaacctgta tcagaacatg cgggccaatg cgcttactga 5580tgcggaatta cgccgtaagg ccgcagatga gcttgtccat atgactgcga gaattaaccg 5640tggtgaggcg atccctgaac cagtaaaaca acttcctgtc atgggcggta gacctctaaa 5700tcgtgcacag gctctggcga agatcgcaga aatcaaagct aagttcggac tgaaaggagc 5760aagtgtatga cgggcaaaga ggcaattatt cattacctgg ggacgcataa tagcttctgt 5820gcgccggacg ttgccgcgct aacaggcgca acagtaacca gcataaatca ggccgcggct 5880aaaatggcac gggcaggtct tctggttatc gaaggtaagg tctggcgaac ggtgtattac 5940cggtttgcta ccagggaaga acgggaagga aagatgagca cgaacctggt ttttaaggag 6000tgtcgccaga gtgccgcgat gaaacgggta ttggcggtat atggagttaa aagatgacca 6060tctacattac tgagctaata acaggcctgc tggtaatcgc aggccttttt atttggggga 6120gagggaagtc atgaaaaaac taacctttga aattcgatct ccagcacatc agcaaaacgc 6180tattcacgca gtacagcaaa tccttccaga cccaaccaaa ccaatcgtag taaccattca 6240ggaacgcaac cgcagcttag accaaaacag gaagctatgg gcctgcttag gtgacgtctc 6300tcgtcaggtt gaatggcatg gtcgctggct ggatgcagaa agctggaagt gtgtgtttac 6360cgcagcatta aagcagcagg atgttgttcc taaccttgcc gggaatggct ttgtggtaat 6420aggccagtca accagcagga tgcgtgtagg cgaatttgcg gagctattag agcttataca 6480ggcattcggt acagagcgtg gcgttaagtg gtcagacgaa gcgagactgg ctctggagtg 6540gaaagcgaga tggggagaca gggctgcatg ataaatgtcg ttagtttctc cggtggcagg 6600acgtcagcat atttgctctg gctaatggag caaaagcgac gggcaggtaa agacgtgcat 6660tacgttttca tggatacagg ttgtgaacat ccaatgacat atcggtttgt cagggaagtt 6720gtgaagttct gggatatacc gctcaccgta ttgcaggttg atatcaaccc ggagcttgga 6780cagccaaatg gttatacggt atgggaacca aaggatattc agacgcgaat gcctgttctg 6840aagccattta tcgatatggt aaagaaatat ggcactccat acgtcggcgg cgcgttctgc 6900actgacagat taaaactcgt tcccttcacc aaatactgtg atgaccattt cgggcgaggg 6960aattacacca cgtggattgg catcagagct gatgaaccga agcggctaaa gccaaagcct 7020ggaatcagat atcttgctga actgtcagac tttgagaagg aagatatcct cgcatggtgg 7080aagcaacaac cattcgattt gcaaataccg gaacatctcg gtaactgcat attctgcatt 7140aaaaaatcaa cgcaaaaaat cggacttgcc tgcaaagatg aggagggatt gcagcgtgtt 7200tttaatgagg tcatcacggg atcccatgtg cgtgacggac atcgggaaac gccaaaggag 7260attatgtacc gaggaagaat gtcgctggac ggtatcgcga aaatgtattc agaaaatgat 7320tatcaagccc tgtatcagga catggtacga gctaaaagat tcgataccgg ctcttgttct 7380gagtcatgcg aaatatttgg agggcagctt gatttcgact tcgggaggga agctgcatga 7440tgcgatgtta tcggtgcggt gaatgcaaag aagataaccg cttccgacca aatcaacctt 7500actggaatcg atggtgtctc cggtgtgaaa gaacaccaac aggggtgtta ccactaccgc 7560aggaaaagga ggacgtgtgg cgagacagcg acgaagtatc accgacataa tctgcgaaaa 7620ctgcaaatac cttccaacga aacgcaccag aaataaaccc aagccaatcc caaaagaatc 7680tgacgtaaaa accttcaact acacggctca cctgtgggat atccggtggc taagacgtcg 7740tgcgaggaaa acaaggtgat tgaccaaaat cgaagttacg aacaagaaag cgtcgagcga 7800gctttaacgt gcgctaactg cggtcagaag ctgcatgtgc tggaagttca cgtgtgtgag 7860cactgctgcg cagaactgat gagcgatccg aatagctcga tgcacgagga agaagatgat 7920ggctaaacca gcgcgaagac gatgtaaaaa cgatgaatgc cgggaatggt ttcaccctgc 7980attcgctaat cagtggtggt gctctccaga gtgtggaacc aagatagcac tcgaacgacg 8040aagtaaagaa cgcgaaaaag cggaaaaagc agcagagaag aaacgacgac gagaggagca 8100gaaacagaaa gataaactta agattcgaaa actcgcctta aagccccgca gttactggat 8160taaacaagcc caacaagccg taaacgcctt catcagagaa agagaccgcg acttaccatg 8220tatctcgtgc ggaacgctca cgtctgctca gtgggatgcc ggacattacc ggacaactgc 8280tgcggcacct caactccgat ttaatgaacg caatattcac aagcaatgcg tggtgtgcaa 8340ccagcacaaa agcggaaatc tcgttccgta tcgcgtcgaa ctgattagcc gcatcgggca 8400ggaagcagta gacgaaatcg aatcaaacca taaccgccat cgctggacta tcgaagagtg 8460caaggcgatc aaggcagagt accaacagaa actcaaagac ctgcgaaata gcagaagtga 8520ggccgcatga cgttctcagt aaaaaccatt ccagacatgc tcgttgaagc atacggaaat 8580cagacagaag tagcacgcag actgaaatgt agtcgcggta cggtcagaaa atacgttgat 8640gataaagacg ggaaaatgca cgccatcgtc aacgacgttc tcatggttca tcgcggatgg 8700agtgaaagag atgcgctatt acgaaaaaat tgatggcagc aaataccgaa atatttgggt 8760agttggcgat ctgcacggat gctacacgaa cctgatgaac aaactggata cgattggatt 8820cgacaacaaa aaagacctgc ttatctcggt gggcgatttg gttgatcgtg gtgcagagaa 8880cgttgaatgc ctggaattaa tcacattccc ctggttcaga gctgtacgtg gaaaccatga 8940gcaaatgatg attgatggct tatcagagcg tggaaacgtt aatcactggc tgcttaatgg 9000cggtggctgg ttctttaatc tcgattacga caaagaaatt ctggctaaag ctcttgccca 9060taaagcagat gaacttccgt taatcatcga actggtgagc aaagataaaa aatatgttat 9120ctgccacgcc gattatccct ttgacgaata cgagtttgga aagccagttg atcatcagca 9180ggtaatctgg aaccgcgaac gaatcagcaa ctcacaaaac gggatcgtga aagaaatcaa 9240aggcgcggac acgttcatct ttggtcatac gccagcagtg aaaccactca agtttgccaa 9300ccaaatgtat atcgataccg gcgcagtgtt ctgcggaaac ctaacattga ttcaggtaca 9360gggagaaggc gcatgagact cgaaagcgta gctaaatttc attcgccaaa aagcccgatg 9420atgagcgact caccacgggc cacggcttct gactctcttt ccggtactga tgtgatggct 9480gctatgggga tggcgcaatc acaagccgga ttcggtatgg ctgcattctg cggtaagcac 9540gaactcagcc agaacgacaa acaaaaggct atcaactatc tgatgcaatt tgcacacaag 9600gtatcgggga aataccgtgg tgtggcaaag cttgaaggaa atactaaggc aaaggtactg 9660caagtgctcg caacattcgc ttatgcggat tattgccgta gtgccgcgac gccgggggca 9720agatgcagag attgccatgg tacaggccgt gcggttgata ttgccaaaac agagctgtgg 9780gggagagttg tcgagaaaga gtgcggaaga tgcaaaggcg tcggctattc aaggatgcca 9840gcaagcgcag catatcgcgc tgtgacgatg ctaatcccaa accttaccca acccacctgg 9900tcacgcactg ttaagccgct gtatgacgct ctggtggtgc aatgccacaa agaagagtca 9960atcgcagaca acattttgaa tgcggtcaca cgttagcagc atgattgcca cggatggcaa 10020catattaacg gcatgatatt gacttattga ataaaattgg gtaaatttga ctcaacgatg 10080ggttaattcg ctcgttgtgg tagtgagatg aaaagaggcg gcgcttacta ccgattccgc 10140ctagttggtc acttcgacgt atcgtctgga actccaacca tcgcaggcag agaggtctgc 10200aaaatgcaat cccgaaacag ttcgcaggta atagttagag cctgcataac ggtttcggga 10260ttttttatat ctgcacaaca ggtaagagca ttgagtcgat aatcgtgaag agtcggcgag 10320cctggttagc cagtgctctt tccgttgtgc tgaattaagc gaataccgga agcagaaccg 10380gatcaccaaa tgcgtacagg cgtcatcgcc gcccagcaac agcacaaccc aaactgagcc 10440gtagccactg tctgtcctga attcattagt aatagttacg ctgcggcctt ttacacatga 10500ccttcgtgaa agcgggtggc aggaggtcgc gctaacaacc tcctgccgtt ttgcccgtgc 10560atatcggtca cgaacaaatc tgattactaa acacagtagc ctggatttgt tctatcagta 10620atcgacctta ttcctaatta aatagagcaa atccccttat tgggggtaag acatgaagat 10680gccagaaaaa catgacctgt tggccgccat

tctcgcggca aaggaacaag gcatcggggc 10740aatccttgcg tttgcaatgg cgtaccttcg cggcagatat aatggcggtg cgtttacaaa 10800aacagtaatc gacgcaacga tgtgcgccat tatcgcctgg ttcattcgtg accttctcga 10860cttcgccgga ctaagtagca atctcgctta tataacgagc gtgtttatcg gctacatcgg 10920tactgactcg attggttcgc ttatcaaacg cttcgctgct aaaaaagccg gagtagaaga 10980tggtagaaat caataatcaa cgtaaggcgt tcctcgatat gctggcgtgg tcggagggaa 11040ctgataacgg acgtcagaaa accagaaatc atggttatga cgtcattgta ggcggagagc 11100tatttactga ttactccgat caccctcgca aacttgtcac gctaaaccca aaactcaaat 11160caacaggcgc cggacgctac cagcttcttt cccgttggtg ggatgcctac cgcaagcagc 11220ttggcctgaa agacttctct ccgaaaagtc aggacgctgt ggcattgcag cagattaagg 11280agcgtggcgc tttacctatg attgatcgtg gtgatatccg tcaggcaatc gaccgttgca 11340gcaatatctg ggcttcactg ccgggcgctg gttatggtca gttcgagcat aaggctgaca 11400gcctgattgc aaaattcaaa gaagcgggcg gaacggtcag agagattgat gtatgagcag 11460agtcaccgcg attatctccg ctctggttat ctgcatcatc gtctgcctgt catgggctgt 11520taatcattac cgtgataacg ccattaccta caaagcccag cgcgacaaaa atgccagaga 11580actgaagctg gcgaacgcgg caattactga catgcagatg cgtcagcgtg atgttgctgc 11640gctcgatgca aaatacacga aggagttagc tgatgctaaa gctgaaaatg atgctctgcg 11700tgatgatgtt gccgctggtc gtcgtcggtt gcacatcaaa gcagtctgtc agtcagtgcg 11760tgaagccacc accgcctccg gcgtggataa tgcagcctcc ccccgactgg cagacaccgc 11820tgaacgggat tatttcaccc tcagagagag gctgatcact atgcaaaaac aactggaagg 11880aacccagaag tatattaatg agcagtgcag atagagttgc ccatatcgat gggcaactca 11940tgcaattatt gtgagcaata cacacgcgct tccagcggag tataaatgcc taaagtaata 12000aaaccgagca atccatttac gaatgtttgc tgggtttctg ttttaacaac attttctgcg 12060ccgccacaaa ttttggctgc atcgacagtt ttcttctgcc caattccaga aacgaagaaa 12120tgatgggtga tggtttcctt tggtgctact gctgccggtt tgttttgaac agtaaacgtc 12180tgttgagcac atcctgtaat aagcagggcc agcgcagtag cgagtagcat ttttttcatg 12240gtgttattcc cgatgctttt tgaagttcgc agaatcgtat gtgtagaaaa ttaaacaaac 12300cctaaacaat gagttgaaat ttcatattgt taatatttat taatgtatgt caggtgcgat 12360gaatcgtcat tgtattcccg gattaactat gtccacagcc ctgacgggga acttctctgc 12420gggagtgtcc gggaataatt aaaacgatgc acacagggtt tagcgcgtac acgtattgca 12480ttatgccaac gccccggtgc tgacacggaa gaaaccggac gttatgattt agcgtggaaa 12540gatttgtgta gtgttctgaa tgctctcagt aaatagtaat gaattatcaa aggtatagta 12600atatctttta tgttcatgga tatttgtaac ccatcggaaa actcctgctt tagcaagatt 12660ttccctgtat tgctgaaatg tgatttctct tgatttcaac ctatcatagg acgtttctat 12720aagatgcgtg tttcttgaga atttaacatt tacaaccttt ttaagtcctt ttattaacac 12780ggtgttatcg ttttctaaca cgatgtgaat attatctgtg gctagatagt aaatataatg 12840tgagacgttg tgacgtttta gttcagaata aaacaattca cagtctaaat cttttcgcac 12900ttgatcgaat atttctttaa aaatggcaac ctgagccatt ggtaaaacct tccatgtgat 12960acgagggcgc gtagtttgca ttatcgtttt tatcgtttca atctggtctg acctccttgt 13020gttttgttga tgatttatgt caaatattag gaatgttttc acttaatagt attggttgcg 13080taacaaagtg cggtcctgct ggcattctgg agggaaatac aaccgacaga tgtatgtaag 13140gccaacgtgc tcaaatcttc atacagaaag atttgaagta atattttaac cgctagatga 13200agagcaagcg catggagcga caaaatgaat aaagaacaat ctgctgatga tccctccgtg 13260gatctgattc gtgtaaaaaa tatgcttaat agcaccattt ctatgagtta ccctgatgtt 13320gtaattgcat gtatagaaca taaggtgtct ctggaagcat tcagagcaat tgaggcagcg 13380ttggtgaagc acgataataa tatgaaggat tattccctgg tggttgactg atcaccataa 13440ctgctaatca ttcaaactat ttagtctgtg acagagccaa cacgcagtct gtcactgtca 13500ggaaagtggt aaaactgcaa ctcaattact gcaatgccct cgtaattaag tgaatttaca 13560atatcgtcct gttcggaggg aagaacgcgg gatgttcatt cttcatcact tttaattgat 13620gtatatgctc tcttttctga cgttagtctc cgacggcagg cttcaatgac ccaggctgag 13680aaattcccgg accctttttg ctcaagagcg atgttaattt gttcaatcat ttggttagga 13740aagcggatgt tgcgggttgt tgttctgcgg gttctgttct tcgttgacat gaggttgccc 13800cgtattcagt gtcgctgatt tgtattgtct gaagttgttt ttacgttaag ttgatgcaga 13860tcaattaata cgatacctgc gtcataattg attatttgac gtggtttgat ggcctccacg 13920cacgttgtga tatgtagatg ataatcatta tcactttacg ggtcctttcc ggtgatccga 13980caggttacgg ggcggcgacc tcgcgggttt tcgctattta tgaaaatttt ccggtttaag 14040gcgtttccgt tcttcttcgt cataacttaa tgtttttatt taaaataccc tctgaaaaga 14100aaggaaacga caggtgctga aagcgaggct ttttggcctc tgtcgtttcc tttctctgtt 14160tttgtccgtg gaatgaacaa tggaagtcaa caaaaagcag ctggctgaca ttttcggtgc 14220gagtatccgt accattcaga actggcagga acagggaatg cccgttctgc gaggcggtgg 14280caagggtaat gaggtgcttt atgactctgc cgccgtcata aaatggtatg ccgaaaggga 14340tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa gaactgcggc aggccagcga 14400ggcagatctc cagccaggaa ctattgagta cgaacgccat cgacttacgc gtgcgcaggc 14460cgacgcacag gaactgaaga atgccagaga ctccgctgaa gtggtggaaa ccgcattctg 14520tactttcgtg ctgtcgcgga tcgcaggtga aattgccagt attctcgacg ggctccccct 14580gtcggtgcag cggcgttttc cggaactgga aaaccgacat gttgatttcc tgaaacggga 14640tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag 14700tgaatatatc gaacagtcag gttaacaggc tgcggcattt tgtccgcgcc gggcttcgct 14760cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc 14820cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca 14880tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg 14940gttattccaa aatgctgctg ggtgtttatg cctactttat agagcataag cagcgcaaca 15000cccttatctg gttgccgacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc 15060cgactattcg tgatattccg tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc 15120gggataacac gctcaccatg aagcgtttca ctaatgggcg tggcttctgg tgcctgggcg 15180gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt ggcgggttat gatgaacttg 15240ctgcttttga tgatgatatt gaacaggaag gctctccgac gttcctgggt gacaagcgta 15300ttgaaggctc ggtctggcca aagtccatcc gtggctccac gccaaaagtg agaggcacct 15360gtcagattga gcgtgcagcc agtgaatccc cgcattttat gcgttttcat gttgcctgcc 15420cgcattgcgg ggaggagcag tatcttaaat ttggcgacaa agagacgccg tttggcctca 15480aatggacgcc ggatgacccc tccagcgtgt tttatctctg cgagcataat gcctgcgtca 15540tccgccagca ggagctggac tttactgatg cccgttatat ctgcgaaaag accgggatct 15600ggacccgtga tggcattctc tggttttcgt catccggtga agagattgag ccacctgaca 15660gtgtgacctt tcacatctgg acagcgtaca gcccgttcac cacctgggtg cagattgtca 15720aagactggat gaaaacgaaa ggggatacgg gaaaacgtaa aaccttcgta aacaccacgc 15780tcggtgagac gtgggaggcg aaaattggcg aacgtccgga tgctgaagtg atggcagagc 15840ggaaagagca ttattcagcg cccgttcctg accgtgtggc ttacctgacc gccggtatcg 15900actcccagct ggaccgctac gaaatgcgcg tatggggatg ggggccgggt gaggaaagct 15960ggctgattga ccggcagatt attatgggcc gccacgacga tgaacagacg ctgctgcgtg 16020tggatgaggc catcaataaa acctataccc gccggaatgg tgcagaaatg tcgatatccc 16080gtatctgctg ggatactggc gggattgacc cgaccattgt gtatgaacgc tcgaaaaaac 16140atgggctgtt ccgggtgatc cccattaaag gggcatccgt ctacggaaag ccggtggcca 16200gcatgccacg taagcgaaac aaaaacgggg tttaccttac cgaaatcggt acggataccg 16260cgaaagagca gatttataac cgcttcacac tgacgccgga aggggatgaa ccgcttcccg 16320gtgccgttca cttcccgaat aacccggata tttttgatct gaccgaagcg cagcagctga 16380ctgctgaaga gcaggtcgaa aaatgggtgg atggcaggaa aaaaatactg tgggacagca 16440aaaagcgacg caatgaggca ctcgactgct tcgtttatgc gctggcggcg ctgcgcatca 16500gtatttcccg ctggcagctg gatctcagtg cgctgctggc gagcctgcag gaagaggatg 16560gtgcagcaac caacaagaaa acactggcag attacgcccg tgccttatcc ggagaggatg 16620aatgacgcga caggaagaac ttgccgctgc ccgtgcggca ctgcatgacc tgatgacagg 16680taaacgggtg gcaacagtac agaaagacgg acgaagggtg gagtttacgg ccacttccgt 16740gtctgacctg aaaaaatata ttgcagagct ggaagtgcag accggcatga cacagcgacg 16800caggggacct gcaggatttt atgtatgaaa acgcccacca ttcccaccct tctggggccg 16860gacggcatga catcgctgcg cgaatatgcc ggttatcacg gcggtggcag cggatttgga 16920gggcagttgc ggtcgtggaa cccaccgagt gaaagtgtgg atgcagccct gttgcccaac 16980tttacccgtg gcaatgcccg cgcagacgat ctggtacgca ataacggcta tgccgccaac 17040gccatccagc tgcatcagga tcatatcgtc gggtcttttt tccggctcag tcatcgccca 17100agctggcgct atctgggcat cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa 17160gcggcatgga aagagtttgc cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc 17220acgtttacca tgatgattcg ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc 17280gttcaggcca cctgggatac cagttcgtcg cggcttttcc ggacacagtt ccggatggtc 17340agcccgaagc gcatcagcaa cccgaacaat accggcgaca gccggaactg ccgtgccggt 17400gtgcagatta atgacagcgg tgcggcgctg ggatattacg tcagcgagga cgggtatcct 17460ggctggatgc cgcagaaatg gacatggata ccccgtgagt tacccggcgg gcgcgcctcg 17520ttcattcacg tttttgaacc cgtggaggac gggcagactc gcggtgcaaa tgtgttttac 17580agcgtgatgg agcagatgaa gatgctcgac acgctgcaga acacgcagct gcagagcgcc 17640attgtgaagg cgatgtatgc cgccaccatt gagagtgagc tggatacgca gtcagcgatg 17700gattttattc tgggcgcgaa cagtcaggag cagcgggaaa ggctgaccgg ctggattggt 17760gaaattgccg cgtattacgc cgcagcgccg gtccggctgg gaggcgcaaa agtaccgcac 17820ctgatgccgg gtgactcact gaacctgcag acggctcagg atacggataa cggctactcc 17880gtgtttgagc agtcactgct gcggtatatc gctgccgggc tgggtgtctc gtatgagcag 17940ctttcccgga attacgccca gatgagctac tccacggcac gggccagtgc gaacgagtcg 18000tgggcgtact ttatggggcg gcgaaaattc gtcgcatccc gtcaggcgag ccagatgttt 18060ctgtgctggc tggaagaggc catcgttcgc cgcgtggtga cgttaccttc aaaagcgcgc 18120ttcagttttc aggaagcccg cagtgcctgg gggaactgcg actggatagg ctccggtcgt 18180atggccatcg atggtctgaa agaagttcag gaagcggtga tgctgataga agccggactg 18240agtacctacg agaaagagtg cgcaaaacgc ggtgacgact atcaggaaat ttttgcccag 18300caggtccgtg aaacgatgga gcgccgtgca gccggtctta aaccgcccgc ctgggcggct 18360gcagcatttg aatccgggct gcgacaatca acagaggagg agaagagtga cagcagagct 18420gcgtaatctc ccgcatattg ccagcatggc ctttaatgag ccgctgatgc ttgaacccgc 18480ctatgcgcgg gttttctttt gtgcgcttgc aggccagctt gggatcagca gcctgacgga 18540tgcggtgtcc ggcgacagcc tgactgccca ggaggcactc gcgacgctgg cattatccgg 18600tgatgatgac ggaccacgac aggcccgcag ttatcaggtc atgaacggca tcgccgtgct 18660gccggtgtcc ggcacgctgg tcagccggac gcgggcgctg cagccgtact cggggatgac 18720cggttacaac ggcattatcg cccgtctgca acaggctgcc agcgatccga tggtggacgg 18780cattctgctc gatatggaca cgcccggcgg gatggtggcg ggggcatttg actgcgctga 18840catcatcgcc cgtgtgcgtg acataaaacc ggtatgggcg cttgccaacg acatgaactg 18900cagtgcaggt cagttgcttg ccagtgccgc ctcccggcgt ctggtcacgc agaccgcccg 18960gacaggctcc atcggcgtca tgatggctca cagtaattac ggtgctgcgc tggagaaaca 19020gggtgtggaa atcacgctga tttacagcgg cagccataag gtggatggca acccctacag 19080ccatcttccg gatgacgtcc gggagacact gcagtcccgg atggacgcaa cccgccagat 19140gtttgcgcag aaggtgtcgg catataccgg cctgtccgtg caggttgtgc tggataccga 19200ggctgcagtg tacagcggtc aggaggccat tgatgccgga ctggctgatg aacttgttaa 19260cagcaccgat gcgatcaccg tcatgcgtga tgcactggat gcacgtaaat cccgtctctc 19320aggagggcga atgaccaaag agactcaatc aacaactgtt tcagccactg cttcgcaggc 19380tgacgttact gacgtggtgc cagcgacgga gggcgagaac gccagcgcgg cgcagccgga 19440cgtgaacgcg cagatcaccg cagcggttgc ggcagaaaac agccgcatta tggggatcct 19500caactgtgag gaggctcacg gacgcgaaga acaggcacgc gtgctggcag aaacccccgg 19560tatgaccgtg aaaacggccc gccgcattct ggccgcagca ccacagagtg cacaggcgcg 19620cagtgacact gcgctggatc gtctgatgca gggggcaccg gcaccgctgg ctgcaggtaa 19680cccggcatct gatgccgtta acgatttgct gaacacacca gtgtaaggga tgtttatgac 19740gagcaaagaa acctttaccc attaccagcc gcagggcaac agtgacccgg ctcataccgc 19800aaccgcgccc ggcggattga gtgcgaaagc gcctgcaatg accccgctga tgctggacac 19860ctccagccgt aagctggttg cgtgggatgg caccaccgac ggtgctgccg ttggcattct 19920tgcggttgct gctgaccaga ccagcaccac gctgacgttc tacaagtccg gcacgttccg 19980ttatgaggat gtgctctggc cggaggctgc cagcgacgag acgaaaaaac ggaccgcgtt 20040tgccggaacg gcaatcagca tcgtttaact ttacccttca tcactaaagg ccgcctgtgc 20100ggcttttttt acgggatttt tttatgtcga tgtacacaac cgcccaactg ctggcggcaa 20160atgagcagaa atttaagttt gatccgctgt ttctgcgtct ctttttccgt gagagctatc 20220ccttcaccac ggagaaagtc tatctctcac aaattccggg actggtaaac atggcgctgt 20280acgtttcgcc gattgtttcc ggtgaggtta tccgttcccg tggcggctcc acctctgaat 20340ttacgccggg atatgtcaag ccgaagcatg aagtgaatcc gcagatgacc ctgcgtcgcc 20400tgccggatga agatccgcag aatctggcgg acccggctta ccgccgccgt cgcatcatca 20460tgcagaacat gcgtgacgaa gagctggcca ttgctcaggt cgaagagatg caggcagttt 20520ctgccgtgct taagggcaaa tacaccatga ccggtgaagc cttcgatccg gttgaggtgg 20580atatgggccg cagtgaggag aataacatca cgcagtccgg cggcacggag tggagcaagc 20640gtgacaagtc cacgtatgac ccgaccgacg atatcgaagc ctacgcgctg aacgccagcg 20700gtgtggtgaa tatcatcgtg ttcgatccga aaggctgggc gctgttccgt tccttcaaag 20760ccgtcaagga gaagctggat acccgtcgtg gctctaattc cgagctggag acagcggtga 20820aagacctggg caaagcggtg tcctataagg ggatgtatgg cgatgtggcc atcgtcgtgt 20880attccggaca gtacgtggaa aacggcgtca aaaagaactt cctgccggac aacacgatgg 20940tgctggggaa cactcaggca cgcggtctgc gcacctatgg ctgcattcag gatgcggacg 21000cacagcgcga aggcattaac gcctctgccc gttacccgaa aaactgggtg accaccggcg 21060atccggcgcg tgagttcacc atgattcagt cagcaccgct gatgctgctg gctgaccctg 21120atgagttcgt gtccgtacaa ctggcgtaat catggccctt cggggccatt gtttctctgt 21180ggaggagtcc atgacgaaag atgaactgat tgcccgtctc cgctcgctgg gtgaacaact 21240gaaccgtgat gtcagcctga cggggacgaa agaagaactg gcgctccgtg tggcagagct 21300gaaagaggag cttgatgaca cggatgaaac tgccggtcag gacacccctc tcagccggga 21360aaatgtgctg accggacatg aaaatgaggt gggatcagcg cagccggata ccgtgattct 21420ggatacgtct gaactggtca cggtcgtggc actggtgaag ctgcatactg atgcacttca 21480cgccacgcgg gatgaacctg tggcatttgt gctgccggga acggcgtttc gtgtctctgc 21540cggtgtggca gccgaaatga cagagcgcgg cctggccaga atgcaataac gggaggcgct 21600gtggctgatt tcgataacct gttcgatgct gccattgccc gcgccgatga aacgatacgc 21660gggtacatgg gaacgtcagc caccattaca tccggtgagc agtcaggtgc ggtgatacgt 21720ggtgtttttg atgaccctga aaatatcagc tatgccggac agggcgtgcg cgttgaaggc 21780tccagcccgt ccctgtttgt ccggactgat gaggtgcggc agctgcggcg tggagacacg 21840ctgaccatcg gtgaggaaaa tttctgggta gatcgggttt cgccggatga tggcggaagt 21900tgtcatctct ggcttggacg gggcgtaccg cctgccgtta accgtcgccg ctgaaagggg 21960gatgtatggc cataaaaggt cttgagcagg ccgttgaaaa cctcagccgt atcagcaaaa 22020cggcggtgcc tggtgccgcc gcaatggcca ttaaccgcgt tgcttcatcc gcgatatcgc 22080agtcggcgtc acaggttgcc cgtgagacaa aggtacgccg gaaactggta aaggaaaggg 22140ccaggctgaa aagggccacg gtcaaaaatc cgcaggccag aatcaaagtt aaccgggggg 22200atttgcccgt aatcaagctg ggtaatgcgc gggttgtcct ttcgcgccgc aggcgtcgta 22260aaaaggggca gcgttcatcc ctgaaaggtg gcggcagcgt gcttgtggtg ggtaaccgtc 22320gtattcccgg cgcgtttatt cagcaactga aaaatggccg gtggcatgtc atgcagcgtg 22380tggctgggaa aaaccgttac cccattgatg tggtgaaaat cccgatggcg gtgccgctga 22440ccacggcgtt taaacaaaat attgagcgga tacggcgtga acgtcttccg aaagagctgg 22500gctatgcgct gcagcatcaa ctgaggatgg taataaagcg atgaaacata ctgaactccg 22560tgcagccgta ctggatgcac tggagaagca tgacaccggg gcgacgtttt ttgatggtcg 22620ccccgctgtt tttgatgagg cggattttcc ggcagttgcc gtttatctca ccggcgctga 22680atacacgggc gaagagctgg acagcgatac ctggcaggcg gagctgcata tcgaagtttt 22740cctgcctgct caggtgccgg attcagagct ggatgcgtgg atggagtccc ggatttatcc 22800ggtgatgagc gatatcccgg cactgtcaga tttgatcacc agtatggtgg ccagcggcta 22860tgactaccgg cgcgacgatg atgcgggctt gtggagttca gccgatctga cttatgtcat 22920tacctatgaa atgtgaggac gctatgcctg taccaaatcc tacaatgccg gtgaaaggtg 22980ccgggaccac cctgtgggtt tataagggga gcggtgaccc ttacgcgaat ccgctttcag 23040acgttgactg gtcgcgtctg gcaaaagtta aagacctgac gcccggcgaa ctgaccgctg 23100agtcctatga cgacagctat ctcgatgatg aagatgcaga ctggactgcg accgggcagg 23160ggcagaaatc tgccggagat accagcttca cgctggcgtg gatgcccgga gagcaggggc 23220agcaggcgct gctggcgtgg tttaatgaag gcgatacccg tgcctataaa atccgcttcc 23280cgaacggcac ggtcgatgtg ttccgtggct gggtcagcag tatcggtaag gcggtgacgg 23340cgaaggaagt gatcacccgc acggtgaaag tcaccaatgt gggacgtccg tcgatggcag 23400aagatcgcag cacggtaaca gcggcaaccg gcatgaccgt gacgcctgcc agcacctcgg 23460tggtgaaagg gcagagcacc acgctgaccg tggccttcca gccggagggc gtaaccgaca 23520agagctttcg tgcggtgtct gcggataaaa caaaagccac cgtgtcggtc agtggtatga 23580ccatcaccgt gaacggcgtt gctgcaggca aggtcaacat tccggttgta tccggtaatg 23640gtgagtttgc tgcggttgca gaaattaccg tcaccgccag ttaatccgga gagtcagcga 23700tgttcctgaa aaccgaatca tttgaacata acggtgtgac cgtcacgctt tctgaactgt 23760cagccctgca gcgcattgag catctcgccc tgatgaaacg gcaggcagaa caggcggagt 23820cagacagcaa ccggaagttt actgtggaag acgccatcag aaccggcgcg tttctggtgg 23880cgatgtccct gtggcataac catccgcaga agacgcagat gccgtccatg aatgaagccg 23940ttaaacagat tgagcaggaa gtgcttacca cctggcccac ggaggcaatt tctcatgctg 24000aaaacgtggt gtaccggctg tctggtatgt atgagtttgt ggtgaataat gcccctgaac 24060agacagagga cgccgggccc gcagagcctg tttctgcggg aaagtgttcg acggtgagct 24120gagttttgcc ctgaaactgg cgcgtgagat ggggcgaccc gactggcgtg ccatgcttgc 24180cgggatgtca tccacggagt atgccgactg gcaccgcttt tacagtaccc attattttca 24240tgatgttctg ctggatatgc acttttccgg gctgacgtac accgtgctca gcctgttttt 24300cagcgatccg gatatgcatc cgctggattt cagtctgctg aaccggcgcg aggctgacga 24360agagcctgaa gatgatgtgc tgatgcagaa agcggcaggg cttgccggag gtgtccgctt 24420tggcccggac gggaatgaag ttatccccgc ttccccggat gtggcggaca tgacggagga 24480tgacgtaatg ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac 24540cggtaggcga tctggtcgtt gatttgagtc tggatgcggc cagatttgac gagcagatgg 24600ccagagtcag gcgtcatttt tctggtacgg aaagtgatgc gaaaaaaaca gcggcagtcg 24660ttgaacagtc gctgagccga caggcgctgg ctgcacagaa agcggggatt tccgtcgggc 24720agtataaagc cgccatgcgt atgctgcctg cacagttcac cgacgtggcc acgcagcttg 24780caggcgggca aagtccgtgg ctgatcctgc tgcaacaggg ggggcaggtg aaggactcct 24840tcggcgggat gatccccatg ttcagggggc ttgccggtgc gatcaccctg ccgatggtgg 24900gggccacctc gctggcggtg gcgaccggtg cgctggcgta tgcctggtat cagggcaact 24960caaccctgtc cgatttcaac aaaacgctgg tcctttccgg caatcaggcg ggactgacgg 25020cagatcgtat gctggtcctg tccagagccg ggcaggcggc agggctgacg tttaaccaga 25080ccagcgagtc actcagcgca ctggttaagg cgggggtaag cggtgaggct cagattgcgt 25140ccatcagcca gagtgtggcg cgtttctcct ctgcatccgg cgtggaggtg gacaaggtcg 25200ctgaagcctt cgggaagctg accacagacc cgacgtcggg gctgacggcg atggctcgcc 25260agttccataa cgtgtcggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg 25320atgaagccgg ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga 25380cccgccgcct gaaagagaac atgggcacgc tggagacctg ggcagacagg actgcgcggg 25440cattcaaatc catgtgggat gcggtgctgg atattggtcg tcctgatacc gcgcaggaga 25500tgctgattaa ggcagaggct gcgtataaga aagcagacga catctggaat ctgcgcaagg 25560atgattattt tgttaacgat gaagcgcggg cgcgttactg ggatgatcgt gaaaaggccc 25620gtcttgcgct tgaagccgcc cgaaagaagg ctgagcagca gactcaacag gacaaaaatg 25680cgcagcagca gagcgatacc gaagcgtcac ggctgaaata taccgaagag gcgcagaagg 25740cttacgaacg gctgcagacg ccgctggaga

aatataccgc ccgtcaggaa gaactgaaca 25800aggcactgaa agacgggaaa atcctgcagg cggattacaa cacgctgatg gcggcggcga 25860aaaaggatta tgaagcgacg ctgaaaaagc cgaaacagtc cagcgtgaag gtgtctgcgg 25920gcgatcgtca ggaagacagt gctcatgctg ccctgctgac gcttcaggca gaactccgga 25980cgctggagaa gcatgccgga gcaaatgaga aaatcagcca gcagcgccgg gatttgtgga 26040aggcggagag tcagttcgcg gtactggagg aggcggcgca acgtcgccag ctgtctgcac 26100aggagaaatc cctgctggcg cataaagatg agacgctgga gtacaaacgc cagctggctg 26160cacttggcga caaggttacg tatcaggagc gcctgaacgc gctggcgcag caggcggata 26220aattcgcaca gcagcaacgg gcaaaacggg ccgccattga tgcgaaaagc cgggggctga 26280ctgaccggca ggcagaacgg gaagccacgg aacagcgcct gaaggaacag tatggcgata 26340atccgctggc gctgaataac gtcatgtcag agcagaaaaa gacctgggcg gctgaagacc 26400agcttcgcgg gaactggatg gcaggcctga agtccggctg gagtgagtgg gaagagagcg 26460ccacggacag tatgtcgcag gtaaaaagtg cagccacgca gacctttgat ggtattgcac 26520agaatatggc ggcgatgctg accggcagtg agcagaactg gcgcagcttc acccgttccg 26580tgctgtccat gatgacagaa attctgctta agcaggcaat ggtggggatt gtcgggagta 26640tcggcagcgc cattggcggg gctgttggtg gcggcgcatc cgcgtcaggc ggtacagcca 26700ttcaggccgc tgcggcgaaa ttccattttg caaccggagg atttacggga accggcggca 26760aatatgagcc agcggggatt gttcaccgtg gtgagtttgt cttcacgaag gaggcaacca 26820gccggattgg cgtggggaat ctttaccggc tgatgcgcgg ctatgccacc ggcggttatg 26880tcggtacacc gggcagcatg gcagacagcc ggtcgcaggc gtccgggacg tttgagcaga 26940ataaccatgt ggtgattaac aacgacggca cgaacgggca gataggtccg gctgctctga 27000aggcggtgta tgacatggcc cgcaagggtg cccgtgatga aattcagaca cagatgcgtg 27060atggtggcct gttctccgga ggtggacgat gaagaccttc cgctggaaag tgaaacccgg 27120tatggatgtg gcttcggtcc cttctgtaag aaaggtgcgc tttggtgatg gctattctca 27180gcgagcgcct gccgggctga atgccaacct gaaaacgtac agcgtgacgc tttctgtccc 27240ccgtgaggag gccacggtac tggagtcgtt tctggaagag cacgggggct ggaaatcctt 27300tctgtggacg ccgccttatg agtggcggca gataaaggtg acctgcgcaa aatggtcgtc 27360gcgggtcagt atgctgcgtg ttgagttcag cgcagagttt gaacaggtgg tgaactgatg 27420caggatatcc ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc ggccagcgtg 27480gtgctctggg aaatcgacct gacagaggtc ggtggagaac gttatttttt ctgtaatgag 27540cagaacgaaa aaggtgagcc ggtcacctgg caggggcgac agtatcagcc gtatcccatt 27600caggggagcg gttttgaact gaatggcaaa ggcaccagta cgcgccccac gctgacggtt 27660tctaacctgt acggtatggt caccgggatg gcggaagata tgcagagtct ggtcggcgga 27720acggtggtcc ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga 27780aacagttacg ccgatccgga gcaggaggtg atcagccgct ggcgcattga gcagtgcagc 27840gaactgagcg cggtgagtgc ctcctttgta ctgtccacgc cgacggaaac ggatggcgct 27900gtttttccgg gacgtatcat gctggccaac acctgcacct ggacctatcg cggtgacgag 27960tgcggttata gcggtccggc tgtcgcggat gaatatgacc agccaacgtc cgatatcacg 28020aaggataaat gcagcaaatg cctgagcggt tgtaagttcc gcaataacgt cggcaacttt 28080ggcggcttcc tttccattaa caaactttcg cagtaaatcc catgacacag acagaatcag 28140cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgcggcttc gtggtaagca 28200cgccggaggg ggaaagatat ttcccctgcg tgaatatctc cggtgagccg gaggctattt 28260ccgtatgtcg ccggaagact ggctgcaggc agaaatgcag ggtgagattg tggcgctggt 28320ccacagccac cccggtggtc tgccctggct gagtgaggcc gaccggcggc tgcaggtgca 28380gagtgatttg ccgtggtggc tggtctgccg ggggacgatt cataagttcc gctgtgtgcc 28440gcatctcacc gggcggcgct ttgagcacgg tgtgacggac tgttacacac tgttccggga 28500tgcttatcat ctggcgggga ttgagatgcc ggactttcat cgtgaggatg actggtggcg 28560taacggccag aatctctatc tggataatct ggaggcgacg gggctgtatc aggtgccgtt 28620gtcagcggca cagccgggcg atgtgctgct gtgctgtttt ggttcatcag tgccgaatca 28680cgccgcaatt tactgcggcg acggcgagct gctgcaccat attcctgaac aactgagcaa 28740acgagagagg tacaccgaca aatggcagcg acgcacacac tccctctggc gtcaccgggc 28800atggcgcgca tctgccttta cggggattta caacgatttg gtcgccgcat cgaccttcgt 28860gtgaaaacgg gggctgaagc catccgggca ctggccacac agctcccggc gtttcgtcag 28920aaactgagcg acggctggta tcaggtacgg attgccgggc gggacgtcag cacgtccggg 28980ttaacggcgc agttacatga gactctgcct gatggcgctg taattcatat tgttcccaga 29040gtcgccgggg ccaagtcagg tggcgtattc cagattgtcc tgggggctgc cgccattgcc 29100ggatcattct ttaccgccgg agccaccctt gcagcatggg gggcagccat tggggccggt 29160ggtatgaccg gcatcctgtt ttctctcggt gccagtatgg tgctcggtgg tgtggcgcag 29220atgctggcac cgaaagccag aactccccgt atacagacaa cggataacgg taagcagaac 29280acctatttct cctcactgga taacatggtt gcccagggca atgttctgcc tgttctgtac 29340ggggaaatgc gcgtggggtc acgcgtggtt tctcaggaga tcagcacggc agacgaaggg 29400gacggtggtc aggttgtggt gattggtcgc tgatgcaaaa tgttttatgt gaaaccgcct 29460gcgggcggtt ttgtcattta tggagcgtga ggaatgggta aaggaagcag taaggggcat 29520accccgcgcg aagcgaagga caacctgaag tccacgcagt tgctgagtgt gatcgatgcc 29580atcagcgaag ggccgattga aggtccggtg gatggcttaa aaagcgtgct gctgaacagt 29640acgccggtgc tggacactga ggggaatacc aacatatccg gtgtcacggt ggtgttccgg 29700gctggtgagc aggagcagac tccgccggag ggatttgaat cctccggctc cgagacggtg 29760ctgggtacgg aagtgaaata tgacacgccg atcacccgca ccattacgtc tgcaaacatc 29820gaccgtctgc gctttacctt cggtgtacag gcactggtgg aaaccacctc aaagggtgac 29880aggaatccgt cggaagtccg cctgctggtt cagatacaac gtaacggtgg ctgggtgacg 29940gaaaaagaca tcaccattaa gggcaaaacc acctcgcagt atctggcctc ggtggtgatg 30000ggtaacctgc cgccgcgccc gtttaatatc cggatgcgca ggatgacgcc ggacagcacc 30060acagaccagc tgcagaacaa aacgctctgg tcgtcataca ctgaaatcat cgatgtgaaa 30120cagtgctacc cgaacacggc actggtcggc gtgcaggtgg actcggagca gttcggcagc 30180cagcaggtga gccgtaatta tcatctgcgc gggcgtattc tgcaggtgcc gtcgaactat 30240aacccgcaga cgcggcaata cagcggtatc tgggacggaa cgtttaaacc ggcatacagc 30300aacaacatgg cctggtgtct gtgggatatg ctgacccatc cgcgctacgg catggggaaa 30360cgtcttggtg cggcggatgt ggataaatgg gcgctgtatg tcatcggcca gtactgcgac 30420cagtcagtgc cggacggctt tggcggcacg gagccgcgca tcacctgtaa tgcgtacctg 30480accacacagc gtaaggcgtg ggatgtgctc agcgatttct gctcggcgat gcgctgtatg 30540ccggtatgga acgggcagac gctgacgttc gtgcaggacc gaccgtcgga taagacgtgg 30600acctataacc gcagtaatgt ggtgatgccg gatgatggcg cgccgttccg ctacagcttc 30660agcgccctga aggaccgcca taatgccgtt gaggtgaact ggattgaccc gaacaacggc 30720tgggagacgg cgacagagct tgttgaagat acgcaggcca ttgcccgtta cggtcgtaat 30780gttacgaaga tggatgcctt tggctgtacc agccgggggc aggcacaccg cgccgggctg 30840tggctgatta aaacagaact gctggaaacg cagaccgtgg atttcagcgt cggcgcagaa 30900gggcttcgcc atgtaccggg cgatgttatt gaaatctgcg atgatgacta tgccggtatc 30960agcaccggtg gtcgtgtgct ggcggtgaac agccagaccc ggacgctgac gctcgaccgt 31020gaaatcacgc tgccatcctc cggtaccgcg ctgataagcc tggttgacgg aagtggcaat 31080ccggtcagcg tggaggttca gtccgtcacc gacggcgtga aggtaaaagt gagccgtgtt 31140cctgacggtg ttgctgaata cagcgtatgg gagctgaagc tgccgacgct gcgccagcga 31200ctgttccgct gcgtgagtat ccgtgagaac gacgacggca cgtatgccat caccgccgtg 31260cagcatgtgc cggaaaaaga ggccatcgtg gataacgggg cgcactttga cggcgaacag 31320agtggcacgg tgaatggtgt cacgccgcca gcggtgcagc acctgaccgc agaagtcact 31380gcagacagcg gggaatatca ggtgctggcg cgatgggaca caccgaaggt ggtgaagggc 31440gtgagtttcc tgctccgtct gaccgtaaca gcggacgacg gcagtgagcg gctggtcagc 31500acggcccgga cgacggaaac cacataccgc ttcacgcaac tggcgctggg gaactacagg 31560ctgacagtcc gggcggtaaa tgcgtggggg cagcagggcg atccggcgtc ggtatcgttc 31620cggattgccg caccggcagc accgtcgagg attgagctga cgccgggcta ttttcagata 31680accgccacgc cgcatcttgc cgtttatgac ccgacggtac agtttgagtt ctggttctcg 31740gaaaagcaga ttgcggatat cagacaggtt gaaaccagca cgcgttatct tggtacggcg 31800ctgtactgga tagccgccag tatcaatatc aaaccgggcc atgattatta cttttatatc 31860cgcagtgtga acaccgttgg caaatcggca ttcgtggagg ccgtcggtcg ggcgagcgat 31920gatgcggaag gttacctgga ttttttcaaa ggcaagataa ccgaatccca tctcggcaag 31980gagctgctgg aaaaagtcga gctgacggag gataacgcca gcagactgga ggagttttcg 32040aaagagtgga aggatgccag tgataagtgg aatgccatgt gggctgtcaa aattgagcag 32100accaaagacg gcaaacatta tgtcgcgggt attggcctca gcatggagga cacggaggaa 32160ggcaaactga gccagtttct ggttgccgcc aatcgtatcg catttattga cccggcaaac 32220gggaatgaaa cgccgatgtt tgtggcgcag ggcaaccaga tattcatgaa cgacgtgttc 32280ctgaagcgcc tgacggcccc caccattacc agcggcggca atcctccggc cttttccctg 32340acaccggacg gaaagctgac cgctaaaaat gcggatatca gtggcagtgt gaatgcgaac 32400tccgggacgc tcagtaatgt gacgatagct gaaaactgta cgataaacgg tacgctgagg 32460gcggaaaaaa tcgtcgggga cattgtaaag gcggcgagcg cggcttttcc gcgccagcgt 32520gaaagcagtg tggactggcc gtcaggtacc cgtactgtca ccgtgaccga tgaccatcct 32580tttgatcgcc agatagtggt gcttccgctg acgtttcgcg gaagtaagcg tactgtcagc 32640ggcaggacaa cgtattcgat gtgttatctg aaagtactga tgaacggtgc ggtgatttat 32700gatggcgcgg cgaacgaggc ggtacaggtg ttctcccgta ttgttgacat gccagcgggt 32760cggggaaacg tgatcctgac gttcacgctt acgtccacac ggcattcggc agatattccg 32820ccgtatacgt ttgccagcga tgtgcaggtt atggtgatta agaaacaggc gctgggcatc 32880agcgtggtct gagtgtgtta cagaggttcg tccgggaacg ggcgttttat tataaaacag 32940tgagaggtga acgatgcgta atgtgtgtat tgccgttgct gtctttgccg cacttgcggt 33000gacagtcact ccggcccgtg cggaaggtgg acatggtacg tttacggtgg gctattttca 33060agtgaaaccg ggtacattgc cgtcgttgtc gggcggggat accggtgtga gtcatctgaa 33120agggattaac gtgaagtacc gttatgagct gacggacagt gtgggggtga tggcttccct 33180ggggttcgcc gcgtcgaaaa agagcagcac agtgatgacc ggggaggata cgtttcacta 33240tgagagcctg cgtggacgtt atgtgagcgt gatggccgga ccggttttac aaatcagtaa 33300gcaggtcagt gcgtacgcca tggccggagt ggctcacagt cggtggtccg gcagtacaat 33360ggattaccgt aagacggaaa tcactcccgg gtatatgaaa gagacgacca ctgccaggga 33420cgaaagtgca atgcggcata cctcagtggc gtggagtgca ggtatacaga ttaatccggc 33480agcgtccgtc gttgttgata ttgcttatga aggctccggc agtggcgact ggcgtactga 33540cggattcatc gttggggtcg gttataaatt ctgattagcc aggtaacaca gtgttatgac 33600agcccgccgg aaccggtggg cttttttgtg gggtgaatat ggcagtaaag atttcaggag 33660tcctgaaaga cggcacagga aaaccggtac agaactgcac cattcagctg aaagccagac 33720gtaacagcac cacggtggtg gtgaacacgg tgggctcaga gaatccggat gaagccgggc 33780gttacagcat ggatgtggag tacggtcagt acagtgtcat cctgcaggtt gacggttttc 33840caccatcgca cgccgggacc atcaccgtgt atgaagattc acaaccgggg acgctgaatg 33900attttctctg tgccatgacg gaggatgatg cccggccgga ggtgctgcgt cgtcttgaac 33960tgatggtgga agaggtggcg cgtaacgcgt ccgtggtggc acagagtacg gcagacgcga 34020agaaatcagc cggcgatgcc agtgcatcag ctgctcaggt cgcggccctt gtgactgatg 34080caactgactc agcacgcgcc gccagcacgt ccgccggaca ggctgcatcg tcagctcagg 34140aagcgtcctc cggcgcagaa gcggcatcag caaaggccac tgaagcggaa aaaagtgccg 34200cagccgcaga gtcctcaaaa aacgcggcgg ccaccagtgc cggtgcggcg aaaacgtcag 34260aaacgaatgc tgcagcgtca caacaatcag ccgccacgtc tgcctccacc gcggccacga 34320aagcgtcaga ggccgccact tcagcacgag atgcggtggc ctcaaaagag gcagcaaaat 34380catcagaaac gaacgcatca tcaagtgccg gtcgtgcagc ttcctcggca acggcggcag 34440aaaattctgc cagggcggca aaaacgtccg agacgaatgc caggtcatct gaaacagcag 34500cggaacggag cgcctctgcc gcggcagacg caaaaacagc ggcggcgggg agtgcgtcaa 34560cggcatccac gaaggcgaca gaggctgcgg gaagtgcggt atcagcatcg cagagcaaaa 34620gtgcggcaga agcggcggca atacgtgcaa aaaattcggc aaaacgtgca gaagatatag 34680cttcagctgt cgcgcttgag gatgcggaca caacgagaaa ggggatagtg cagctcagca 34740gtgcaaccaa cagcacgtct gaaacgcttg ctgcaacgcc aaaggcggtt aaggtggtaa 34800tggatgaaac gaacagaaaa gcccactgga cagtccggca ctgaccggaa cgccaacagc 34860accaaccgcg ctcaggggaa caaacaatac ccagattgcg aacaccgctt ttgtactggc 34920cgcgattgca gatgttatcg acgcgtcacc tgacgcactg aatacgctga atgaactggc 34980cgcagcgctc gggaatgatc cagattttgc taccaccatg actaacgcgc ttgcgggtaa 35040acaaccgaag aatgcgacac tgacggcgct ggcagggctt tccacggcga aaaataaatt 35100accgtatttt gcggaaaatg atgccgccag cctgactgaa ctgactcagg ttggcaggga 35160tattctggca aaaaattccg ttgcagatgt tcttgaatac cttggggccg gtgagaattc 35220ggcctttccg gcaggtgcgc cgatcccgtg gccatcagat atcgttccgt ctggctacgt 35280cctgatgcag gggcaggcgt ttgacaaatc agcctaccca aaacttgctg tcgcgtatcc 35340atcgggtgtg cttcctgata tgcgaggctg gacaatcaag gggaaacccg ccagcggtcg 35400tgctgtattg tctcaggaac aggatggaat taagtcgcac acccacagtg ccagtgcatc 35460cggtacggat ttggggacga aaaccacatc gtcgtttgat tacgggacga aaacaacagg 35520cagtttcgat tacggcacca aatcgacgaa taacacgggg gctcatgctc acagtctgag 35580cggttcaaca ggggccgcgg gtgctcatgc ccacacaagt ggtttaagga tgaacagttc 35640tggctggagt cagtatggaa cagcaaccat tacaggaagt ttatccacag ttaaaggaac 35700cagcacacag ggtattgctt atttatcgaa aacggacagt cagggcagcc acagtcactc 35760attgtccggt acagccgtga gtgccggtgc acatgcgcat acagttggta ttggtgcgca 35820ccagcatccg gttgttatcg gtgctcatgc ccattctttc agtattggtt cacacggaca 35880caccatcacc gttaacgctg cgggtaacgc ggaaaacacc gtcaaaaaca ttgcatttaa 35940ctatattgtg aggcttgcat aatggcattc agaatgagtg aacaaccacg gaccataaaa 36000atttataatc tgctggccgg aactaatgaa tttattggtg aaggtgacgc atatattccg 36060cctcataccg gtctgcctgc aaacagtacc gatattgcac cgccagatat tccggctggc 36120tttgtggctg ttttcaacag tgatgaggca tcgtggcatc tcgttgaaga ccatcggggt 36180aaaaccgtct atgacgtggc ttccggcgac gcgttattta tttctgaact cggtccgtta 36240ccggaaaatt ttacctggtt atcgccggga ggggaatatc agaagtggaa cggcacagcc 36300tgggtgaagg atacggaagc agaaaaactg ttccggatcc gggaggcgga agaaacaaaa 36360aaaagcctga tgcaggtagc cagtgagcat attgcgccgc ttcaggatgc tgcagatctg 36420gaaattgcaa cgaaggaaga aacctcgttg ctggaagcct ggaagaagta tcgggtgttg 36480ctgaaccgtg ttgatacatc aactgcacct gatattgagt ggcctgctgt ccctgttatg 36540gagtaatcgt tttgtgatat gccgcagaaa cgttgtatga aataacgttc tgcggttagt 36600tagtatattg taaagctgag tattggttta tttggcgatt attatcttca ggagaataat 36660ggaagttcta tgactcaatt gttcatagtg tttacatcac cgccaattgc ttttaagact 36720gaacgcatga aatatggttt ttcgtcatgt tttgagtctg ctgttgatat ttctaaagtc 36780ggtttttttt cttcgttttc tctaactatt ttccatgaaa tacatttttg attattattt 36840gaatcaattc caattacctg aagtctttca tctataattg gcattgtatg tattggttta 36900ttggagtaga tgcttgcttt tctgagccat agctctgata tccaaatgaa gccataggca 36960tttgttattt tggctctgtc agctgcataa cgccaaaaaa tatatttatc tgcttgatct 37020tcaaatgttg tattgattaa atcaattgga tggaattgtt tatcataaaa aattaatgtt 37080tgaatgtgat aaccgtcctt taaaaaagtc gtttctgcaa gcttggctgt atagtcaact 37140aactcttctg tcgaagtgat atttttaggc ttatctacca gttttagacg ctctttaata 37200tcttcaggaa ttattttatt gtcatattgt atcatgctaa atgacaattt gcttatggag 37260taatctttta attttaaata agttattctc ctggcttcat caaataaaga gtcgaatgat 37320gttggcgaaa tcacatcgtc acccattgga ttgtttattt gtatgccaag agagttacag 37380cagttataca ttctgccata gattatagct aaggcatgta ataattcgta atcttttagc 37440gtattagcga cccatcgtct ttctgattta ataatagatg attcagttaa atatgaaggt 37500aatttctttt gtgcaagtct gactaacttt tttataccaa tgtttaacat actttcattt 37560gtaataaact caatgtcatt ttcttcaatg taagatgaaa taagagtagc ctttgcctcg 37620ctatacattt ctaaatcgcc ttgtttttct atcgtattgc gagaattttt agcccaagcc 37680attaatggat catttttcca tttttcaata acattattgt tataccaaat gtcatatcct 37740ataatctggt ttttgttttt ttgaataata aatgttactg ttcttgcggt ttggaggaat 37800tgattcaaat tcaagcgaaa taattcaggg tcaaaatatg tatcaatgca gcatttgagc 37860aagtgcgata aatctttaag tcttctttcc catggttttt tagtcataaa actctccatt 37920ttgataggtt gcatgctaga tgctgatata ttttagaggt gataaaatta actgcttaac 37980tgtcaatgta atacaagttg tttgatcttt gcaatgattc ttatcagaaa ccatatagta 38040aattagttac acaggaaatt tttaatatta ttattatcat tcattatgta ttaaaattag 38100agttgtggct tggctctgct aacacgttgc tcataggaga tatggtagag ccgcagacac 38160gtcgtatgca ggaacgtgct gcggctggct ggtgaacttc cgatagtgcg ggtgttgaat 38220gatttccagt tgctaccgat tttacatatt ttttgcatga gagaatttgt accacctccc 38280accgaccatc tatgactgta cgccactgtc cctaggactg ctatgtgccg gagcggacat 38340tacaaacgtc cttctcggtg catgccactg ttgccaatga cctgcctagg aattggttag 38400caagttacta ccggattttg taaaaacagc cctcctcata taaaaagtat tcgttcactt 38460ccgataagcg tcgtaatttt ctatctttca tcatattcta gatccctctg aaaaaatctt 38520ccgagtttgc taggcactga tacataactc ttttccaata attggggaag tcattcaaat 38580ctataatagg tttcagattt gcttcaataa attctgactg tagctgctga aacgttgcgg 38640ttgaactata tttccttata acttttacga aagagtttct ttgagtaatc acttcactca 38700agtgcttccc tgcctccaaa cgatacctgt tagcaatatt taatagcttg aaatgatgaa 38760gagctctgtg tttgtcttcc tgcctccagt tcgccgggca ttcaacataa aaactgatag 38820cacccggagt tccggaaacg aaatttgcat atacccattg ctcacgaaaa aaaatgtcct 38880tgtcgatata gggatgaatc gcttggtgta cctcatctac tgcgaaaact tgacctttct 38940ctcccatatt gcagtcgcgg cacgatggaa ctaaattaat aggcatcacc gaaaattcag 39000gataatgtgc aataggaaga aaatgatcta tattttttgt ctgtcctata tcaccacaaa 39060atggacattt ttcacctgat gaaacaagca tgtcatcgta atatgttcta gcgggtttgt 39120ttttatctcg gagattattt tcataaagct tttctaattt aacctttgtc aggttaccaa 39180ctactaaggt tgtaggctca agagggtgtg tcctgtcgta ggtaaataac tgacctgtcg 39240agcttaatat tctatattgt tgttctttct gcaaaaaagt ggggaagtga gtaatgaaat 39300tatttctaac atttatctgc atcatacctt ccgagcattt attaagcatt tcgctataag 39360ttctcgctgg aagaggtagt tttttcattg tactttacct tcatctctgt tcattatcat 39420cgcttttaaa acggttcgac cttctaatcc tatctgacca ttataatttt ttagaatggt 39480ttcataagaa agctctgaat caacggactg cgataataag tggtggtatc cagaatttgt 39540cacttcaagt aaaaacacct cacgagttaa aacacctaag ttctcaccga atgtctcaat 39600atccggacgg ataatattta ttgcttctct tgaccgtagg actttccaca tgcaggattt 39660tggaacctct tgcagtacta ctggggaatg agttgcaatt attgctacac cattgcgtgc 39720atcgagtaag tcgcttaatg ttcgtaaaaa agcagagagc aaaggtggat gcagatgaac 39780ctctggttca tcgaataaaa ctaatgactt ttcgccaacg acatctacta atcttgtgat 39840agtaaataaa acaattgcat gtccagagct cattcgaagc agatatttct ggatattgtc 39900ataaaacaat ttagtgaatt tatcatcgtc cacttgaatc tgtggttcat tacgtcttaa 39960ctcttcatat ttagaaatga ggctgatgag ttccatattt gaaaagtttt catcactact 40020tagttttttg atagcttcaa gccagagttg tctttttcta tctactctca tacaaccaat 40080aaatgctgaa atgaattcta agcggagatc gcctagtgat tttaaactat tgctggcagc 40140attcttgagt ccaatataaa agtattgtgt accttttgct gggtcaggtt gttctttagg 40200aggagtaaaa ggatcaaatg cactaaacga aactgaaaca agcgatcgaa aatatccctt 40260tgggattctt gactcgataa gtctattatt ttcagagaaa aaatattcat tgttttctgg 40320gttggtgatt gcaccaatca ttccattcaa aattgttgtt ttaccacacc cattccgccc 40380gataaaagca tgaatgttcg tgctgggcat agaattaacc gtcacctcaa aaggtatagt 40440taaatcactg aatccgggag cactttttct attaaatgaa aagtggaaat ctgacaattc 40500tggcaaacca tttaacacac gtgcgaactg tccatgaatt tctgaaagag ttacccctct 40560aagtaatgag gtgttaagga cgctttcatt ttcaatgtcg gctaatcgat ttggccatac 40620tactaaatcc tgaatagctt taagaaggtt atgtttaaaa ccatcgctta atttgctgag 40680attaacatag tagtcaatgc tttcacctaa ggaaaaaaac atttcaggga gttgactgaa 40740ttttttatct attaatgaat aagtgcttac ttcttctttt tgacctacaa aaccaatttt 40800aacatttccg atatcgcatt tttcaccatg

ctcatcaaag acagtaagat aaaacattgt 40860aacaaaggaa tagtcattcc aaccatctgc tcgtaggaat gccttatttt tttctactgc 40920aggaatatac ccgcctcttt caataacact aaactccaac atatagtaac ccttaatttt 40980attaaaataa ccgcaattta tttggcggca acacaggatc tctcttttaa gttactctct 41040attacatacg ttttccatct aaaaattagt agtattgaac ttaacggggc atcgtattgt 41100agttttccat atttagcttt ctgcttcctt ttggataacc cactgttatt catgttgcat 41160ggtgcactgt ttataccaac gatatagtct attaatgcat atatagtatc gccgaacgat 41220tagctcttca ggcttctgaa gaagcgtttc aagtactaat aagccgatag atagccacgg 41280acttcgtagc catttttcat aagtgttaac ttccgctcct cgctcataac agacattcac 41340tacagttatg gcggaaaggt atgcatgctg ggtgtgggga agtcgtgaaa gaaaagaagt 41400cagctgcgtc gtttgacatc actgctatct tcttactggt tatgcaggtc gtagtgggtg 41460gcacacaaag ctttgcactg gattgcgagg ctttgtgctt ctctggagtg cgacaggttt 41520gatgacaaaa aattagcgca agaagacaaa aatcaccttg cgctaatgct ctgttacagg 41580tcactaatac catctaagta gttgattcat agtgactgca tatgttgtgt tttacagtat 41640tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac 41700gtttctcgtt cagctttttt atactaagtt ggcattataa aaaagcattg cttatcaatt 41760tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgatt tcaattttgt 41820cccactccct gcctctgtca tcacgatact gtgatgccat ggtgtccgac ttatgcccga 41880gaagatgttg agcaaactta tcgcttatct gcttctcata gagtcttgca gacaaactgc 41940gcaactcgtg aaaggtaggc ggatcccctt cgaaggaaag acctgatgct tttcgtgcgc 42000gcataaaata ccttgatact gtgccggatg aaagcggttc gcgacgagta gatgcaatta 42060tggtttctcc gccaagaatc tctttgcatt tatcaagtgt ttccttcatt gatattccga 42120gagcatcaat atgcaatgct gttgggatgg caatttttac gcctgttttg ctttgctcga 42180cataaagata tccatctacg atatcagacc acttcatttc gcataaatca ccaactcgtt 42240gcccggtaac aacagccagt tccattgcaa gtctgagcca acatggtgat gattctgctg 42300cttgataaat tttcaggtat tcgtcagccg taagtcttga tctccttacc tctgattttg 42360ctgcgcgagt ggcagcgaca tggtttgttg ttatatggcc ttcagctatt gcctctcgga 42420atgcatcgct cagtgttgat ctgattaact tggctgacgc cgccttgccc tcgtctatgt 42480atccattgag cattgccgca atttcttttg tggtgatgtc ttcaagtgga gcatcaggca 42540gacccctcct tattgcttta attttgctca tgtaatttat gagtgtcttc tgcttgattc 42600ctctgctggc caggattttt tcgtagcgat caagccatga atgtaacgta acggaattat 42660cactgttgat tctcgctgtc agaggcttgt gtttgtgtcc tgaaaataac tcaatgttgg 42720cctgtatagc ttcagtgatt gcgattcgcc tgtctctgcc taatccaaac tctttacccg 42780tccttgggtc cctgtagcag taatatccat tgtttcttat ataaaggtta gggggtaaat 42840cccggcgctc atgacttcgc cttcttccca tttctgatcc tcttcaaaag gccacctgtt 42900actggtcgat ttaagtcaac ctttaccgct gattcgtgga acagatactc tcttccatcc 42960ttaaccggag gtgggaatat cctgcattcc cgaacccatc gacgaactgt ttcaaggctt 43020cttggacgtc gctggcgtgc gttccactcc tgaagtgtca agtacatcgc aaagtctccg 43080caattacacg caagaaaaaa ccgccatcag gcggcttggt gttctttcag ttcttcaatt 43140cgaatattgg ttacgtctgc atgtgctatc tgcgcccata tcatccagtg gtcgtagcag 43200tcgttgatgt tctccgcttc gataactctg ttgaatggct ctccattcca ttctcctgtg 43260actcggaagt gcatttatca tctccataaa acaaaacccg ccgtagcgag ttcagataaa 43320ataaatcccc gcgagtgcga ggattgttat gtaatattgg gtttaatcat ctatatgttt 43380tgtacagaga gggcaagtat cgtttccacc gtactcgtga taataatttt gcacggtatc 43440agtcatttct cgcacattgc agaatgggga tttgtcttca ttagacttat aaaccttcat 43500ggaatatttg tatgccgact ctatatctat accttcatct acataaacac cttcgtgatg 43560tctgcatgga gacaagacac cggatctgca caacattgat aacgcccaat ctttttgctc 43620agactctaac tcattgatac tcatttataa actccttgca atgtatgtcg tttcagctaa 43680acggtatcag caatgtttat gtaaagaaac agtaagataa tactcaaccc gatgtttgag 43740tacggtcatc atctgacact acagactctg gcatcgctgt gaagacgacg cgaaattcag 43800cattttcaca agcgttatct tttacaaaac cgatctcact ctcctttgat gcgaatgcca 43860gcgtcagaca tcatatgcag atactcacct gcatcctgaa cccattgacc tccaaccccg 43920taatagcgat gcgtaatgat gtcgatagtt actaacgggt cttgttcgat taactgccgc 43980agaaactctt ccaggtcacc agtgcagtgc ttgataacag gagtcttccc aggatggcga 44040acaacaagaa actggtttcc gtcttcacgg acttcgttgc tttccagttt agcaatacgc 44100ttactcccat ccgagataac accttcgtaa tactcacgct gctcgttgag ttttgatttt 44160gctgtttcaa gctcaacacg cagtttccct actgttagcg caatatcctc gttctcctgg 44220tcgcggcgtt tgatgtattg ctggtttctt tcccgttcat ccagcagttc cagcacaatc 44280gatggtgtta ccaattcatg gaaaaggtct gcgtcaaatc cccagtcgtc atgcattgcc 44340tgctctgccg cttcacgcag tgcctgagag ttaatttcgc tcacttcgaa cctctctgtt 44400tactgataag ttccagatcc tcctggcaac ttgcacaagt ccgacaaccc tgaacgacca 44460ggcgtcttcg ttcatctatc ggatcgccac actcacaaca atgagtggca gatatagcct 44520ggtggttcag gcggcgcatt tttattgctg tgttgcgctg taattcttct atttctgatg 44580ctgaatcaat gatgtctgcc atctttcatt aatccctgaa ctgttggtta atacgcttga 44640gggtgaatgc gaataataaa aaaggagcct gtagctccct gatgattttg cttttcatgt 44700tcatcgttcc ttaaagacgc cgtttaacat gccgattgcc aggcttaaat gagtcggtgt 44760gaatcccatc agcgttaccg tttcgcggtg cttcttcagt acgctacggc aaatgtcatc 44820gacgttttta tccggaaact gctgtctggc tttttttgat ttcagaatta gcctgacggg 44880caatgctgcg aagggcgttt tcctgctgag gtgtcattga acaagtccca tgtcggcaag 44940cataagcaca cagaatatga agcccgctgc cagaaaaatg cattccgtgg ttgtcatacc 45000tggtttctct catctgcttc tgctttcgcc accatcattt ccagcttttg tgaaagggat 45060gcggctaacg tatgaaattc ttcgtctgtt tctactggta ttggcacaaa cctgattcca 45120atttgagcaa ggctatgtgc catctcgata ctcgttctta actcaacaga agatgctttg 45180tgcatacagc ccctcgttta ttatttatct cctcagccag ccgctgtgct ttcagtggat 45240ttcggataac agaaaggccg ggaaataccc agcctcgctt tgtaacggag tagacgaaag 45300tgattgcgcc tacccggata ttatcgtgag gatgcgtcat cgccattgct ccccaaatac 45360aaaaccaatt tcagccagtg cctcgtccat tttttcgatg aactccggca cgatctcgtc 45420aaaactcgcc atgtactttt catcccgctc aatcacgaca taatgcaggc cttcacgctt 45480catacgcggg tcatagttgg caaagtacca ggcatttttt cgcgtcaccc acatgctgta 45540ctgcacctgg gccatgtaag ctgactttat ggcctcgaaa ccaccgagcc ggaacttcat 45600gaaatcccgg gaggtaaacg ggcatttcag ttcaaggccg ttgccgtcac tgcataaacc 45660atcgggagag caggcggtac gcatactttc gtcgcgatag atgatcgggg attcagtaac 45720attcacgccg gaagtgaatt caaacagggt tctggcgtcg ttctcgtact gttttcccca 45780ggccagtgct ttagcgttaa cttccggagc cacaccggtg caaacctcag caagcagggt 45840gtggaagtag gacattttca tgtcaggcca cttctttccg gagcggggtt ttgctatcac 45900gttgtgaact tctgaagcgg tgatgacgcc gagccgtaat ttgtgccacg catcatcccc 45960ctgttcgaca gctctcacat cgatcccggt acgctgcagg ataatgtccg gtgtcatgct 46020gccaccttct gctctgcggc tttctgtttc aggaatccaa gagcttttac tgcttcggcc 46080tgtgtcagtt ctgacgatgc acgaatgtcg cggcgaaata tctgggaaca gagcggcaat 46140aagtcgtcat cccatgtttt atccagggcg atcagcagag tgttaatctc ctgcatggtt 46200tcatcgttaa ccggagtgat gtcgcgttcc ggctgacgtt ctgcagtgta tgcagtattt 46260tcgacaatgc gctcggcttc atccttgtca tagataccag caaatccgaa ggccagacgg 46320gcacactgaa tcatggcttt atgacgtaac atccgtttgg gatgcgactg ccacggcccc 46380gtgatttctc tgccttcgcg agttttgaat ggttcgcggc ggcattcatc catccattcg 46440gtaacgcaga tcggatgatt acggtccttg cggtaaatcc ggcatgtaca ggattcattg 46500tcctgctcaa agtccatgcc atcaaactgc tggttttcat tgatgatgcg ggaccagcca 46560tcaacgccca ccaccggaac gatgccattc tgcttatcag gaaaggcgta aatttctttc 46620gtccacggat taaggccgta ctggttggca acgatcagta atgcgatgaa ctgcgcatcg 46680ctggcatcac ctttaaatgc cgtctggcga agagtggtga tcagttcctg tgggtcgaca 46740gaatccatgc cgacacgttc agccagcttc ccagccagcg ttgcgagtgc agtactcatt 46800cgttttatac ctctgaatca atatcaacct ggtggtgagc aatggtttca accatgtacc 46860ggatgtgttc tgccatgcgc tcctgaaact caacatcgtc atcaaacgca cgggtaatgg 46920attttttgct ggccccgtgg cgttgcaaat gatcgatgca tagcgattca aacaggtgct 46980ggggcaggcc tttttccatg tcgtctgcca gttctgcctc tttctcttca cgggcgagct 47040gctggtagtg acgcgcccag ctctgagcct caagacgatc ctgaatgtaa taagcgttca 47100tggctgaact cctgaaatag ctgtgaaaat atcgcccgcg aaatgccggg ctgattagga 47160aaacaggaaa gggggttagt gaatgctttt gcttgatctc agtttcagta ttaatatcca 47220ttttttataa gcgtcgacgg cttcacgaaa catcttttca tcgccaataa aagtggcgat 47280agtgaattta gtctggatag ccataagtgt ttgatccatt ctttgggact cctggctgat 47340taagtatgtc gataaggcgt ttccatccgt cacgtaattt acgggtgatt cgttcaagta 47400aagattcgga agggcagcca gcaacaggcc accctgcaat ggcatattgc atggtgtgct 47460ccttatttat acataacgaa aaacgcctcg agtgaagcgt tattggtatg cggtaaaacc 47520gcactcaggc ggccttgata gtcatatcat ctgaatcaaa tattcctgat gtatcgatat 47580cggtaattct tattccttcg ctaccatcca ttggaggcca tccttcctga ccatttccat 47640cattccagtc gaactcacac acaacaccat atgcatttaa gtcgcttgaa attgctataa 47700gcagagcatg ttgcgccagc atgattaata cagcatttaa tacagagccg tgtttattga 47760gtcggtattc agagtctgac cagaaattat taatctggtg aagtttttcc tctgtcatta 47820cgtcatggtc gatttcaatt tctattgatg ctttccagtc gtaatcaatg atgtattttt 47880tgatgtttga catctgttca tatcctcaca gataaaaaat cgccctcaca ctggagggca 47940aagaagattt ccaataatca gaacaagtcg gctcctgttt agttacgagc gacattgctc 48000cgtgtattca ctcgttggaa tgaatacaca gtgcagtgtt tattctgtta tttatgccaa 48060aaataaaggc cactatcagg cagctttgtt gttctgttta ccaagttctc tggcaatcat 48120tgccgtcgtt cgtattgccc atttatcgac atatttccca tcttccatta caggaaacat 48180ttcttcaggc ttaaccatgc attccgattg cagcttgcat ccattgcatc gcttgaattg 48240tccacaccat tgatttttat caatagtcgt agtcatacgg atagtcctgg tattgttcca 48300tcacatcctg aggatgctct tcgaactctt caaattcttc ttccatatat caccttaaat 48360agtggattgc ggtagtaaag attgtgcctg tcttttaacc acatcaggct cggtggttct 48420cgtgtacccc tacagcgaga aatcggataa actattacaa cccctacagt ttgatgagta 48480tagaaatgga tccactcgtt attctcggac gagtgttcag taatgaacct ctggagagaa 48540ccatgtatat gatcgttatc tgggttggac ttctgctttt aagcccagat aactggcctg 48600aatatgttaa tgagagaatc ggtattcctc atgtgtggca tgttttcgtc tttgctcttg 48660cattttcgct agcaattaat gtgcatcgat tatcagctat tgccagcgcc agatataagc 48720gatttaagct aagaaaacgc attaagatgc aaaacgataa agtgcgatca gtaattcaaa 48780accttacaga agagcaatct atggttttgt gcgcagccct taatgaaggc aggaagtatg 48840tggttacatc aaaacaattc ccatacatta gtgagttgat tgagcttggt gtgttgaaca 48900aaactttttc ccgatggaat ggaaagcata tattattccc tattgaggat atttactgga 48960ctgaattagt tgccagctat gatccatata atattgagat aaagccaagg ccaatatcta 49020agtaactaga taagaggaat cgattttccc ttaattttct ggcgtccact gcatgttatg 49080ccgcgttcgc caggcttgct gtaccatgtg cgctgattct tgcgctcaat acgttgcagg 49140ttgctttcaa tctgtttgtg gtattcagcc agcactgtaa ggtctatcgg atttagtgcg 49200ctttctactc gtgatttcgg tttgcgattc agcgagagaa tagggcggtt aactggtttt 49260gcgcttaccc caaccaacag gggatttgct gctttccatt gagcctgttt ctctgcgcga 49320cgttcgcggc ggcgtgtttg tgcatccatc tggattctcc tgtcagttag ctttggtggt 49380gtgtggcagt tgtagtcctg aacgaaaacc ccccgcgatt ggcacattgg cagctaatcc 49440ggaatcgcac ttacggccaa tgcttcgttt cgtatcacac accccaaagc cttctgcttt 49500gaatgctgcc cttcttcagg gcttaatttt taagagcgtc accttcatgg tggtcagtgc 49560gtcctgctga tgtgctcagt atcaccgcca gtggtattta tgtcaacacc gccagagata 49620atttatcacc gcagatggtt atctgtatgt tttttatatg aatttatttt ttgcaggggg 49680gcattgtttg gtaggtgaga gatctgaatt gctatgttta gtgagttgta tctatttatt 49740tttcaataaa tacaattggt tatgtgtttt gggggcgatc gtgaggcaaa gaaaacccgg 49800cgctgaggcc gggttattct tgttctctgg tcaaattata tagttggaaa acaaggatgc 49860atatatgaat gaacgatgca gaggcaatgc cgatggcgat agtgggtatc atgtagccgc 49920ttatgctgga aagaagcaat aacccgcaga aaaacaaagc tccaagctca acaaaactaa 49980gggcatagac aataactacc gatgtcatat acccatactc tctaatcttg gccagtcggc 50040gcgttctgct tccgattaga aacgtcaagg cagcaatcag gattgcaatc atggttcctg 50100catatgatga caatgtcgcc ccaagaccat ctctatgagc tgaaaaagaa acaccaggaa 50160tgtagtggcg gaaaaggaga tagcaaatgc ttacgataac gtaaggaatt attactatgt 50220aaacaccagg catgattctg ttccgcataa ttactcctga taattaatcc ttaactttgc 50280ccacctgcct tttaaaacat tccagtatat cacttttcat tcttgcgtag caatatgcca 50340tctcttcagc tatctcagca ttggtgacct tgttcagagg cgctgagaga tggccttttt 50400ctgatagata atgttctgtt aaaatatctc cggcctcatc ttttgcccgc aggctaatgt 50460ctgaaaattg aggtgacggg ttaaaaataa tatccttggc aacctttttt atatcccttt 50520taaattttgg cttaatgact atatccaatg agtcaaaaag ctccccttca atatctgttg 50580cccctaagac ctttaatata tcgccaaata caggtagctt ggcttctacc ttcaccgttg 50640ttcggccgat gaaatgcata tgcataacat cgtctttggt ggttcccctc atcagtggct 50700ctatctgaac gcgctctcca ctgcttaatg acattccttt cccgattaaa aaatctgtca 50760gatcggatgt ggtcggcccg aaaacagttc tggcaaaacc aatggtgtcg ccttcaacaa 50820acaaaaaaga tgggaatccc aatgattcgt catctgcgag gctgttctta atatcttcaa 50880ctgaagcttt agagcgattt atcttctgaa ccagactctt gtcatttgtt ttggtaaaga 50940gaaaagtttt tccatcgatt ttatgaatat acaaataatt ggagccaacc tgcaggtgat 51000gattatcagc cagcagagaa ttaaggaaaa cagacaggtt tattgagcgc ttatctttcc 51060ctttattttt gctgcggtaa gtcgcataaa aaccattctt cataattcaa tccatttact 51120atgttatgtt ctgaggggag tgaaaattcc cctaattcga tgaagattct tgctcaattg 51180ttatcagcta tgcgccgacc agaacacctt gccgatcagc caaacgtctc ttcaggccac 51240tgactagcga taactttccc cacaacggaa caactctcat tgcatgggat cattgggtac 51300tgtgggttta gtggttgtaa aaacacctga ccgctatccc tgatcagttt cttgaaggta 51360aactcatcac ccccaagtct ggctatgcag aaatcacctg gctcaacagc ctgctcaggg 51420tcaacgagaa ttaacattcc gtcaggaaag cttggcttgg agcctgttgg tgcggtcatg 51480gaattacctt caacctcaag ccagaatgca gaatcactgg cttttttggt tgtgcttacc 51540catctctccg catcaccttt ggtaaaggtt ctaagctcag gtgagaacat ccctgcctga 51600acatgagaaa aaacagggta ctcatactca cttctaagtg acggctgcat actaaccgct 51660tcatacatct cgtagatttc tctggcgatt gaagggctaa attcttcaac gctaactttg 51720agaatttttg caagcaatgc ggcgttataa gcatttaatg cattgatgcc attaaataaa 51780gcaccaacgc ctgactgccc catccccatc ttgtctgcga cagattcctg ggataagcca 51840agttcatttt tctttttttc ataaattgct ttaaggcgac gtgcgtcctc aagctgctct 51900tgtgttaatg gtttcttttt tgtgctcata cgttaaatct 51940525DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 5gctttcgaat ttctgccatt catcc 25639DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 6ttgggcgcca tctccttggg ccaacttttg gcgaaaatg 39747DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 7cgttccggct aagtaacatg aaaaagacag ctatcgcgat tgcagtg 47815DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 8tgcccgcggc ctgcg 15927DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 9gcgcctggtc tgtacaccgt tcatctg 271020DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 10catgttactt agccggaacg 201131DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 11cgcaggccgc gggcgcaagc agcctgagac a 311220DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 12tgcggccgcc tgggccccct 201336DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 13agggggccca ggcggccgca gagcaggtcg cggatt 361420DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 14aagcgcagtc tctgaattta 201550DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 15catggcaagc agcctgagac agattctgga ctcccagaaa atggagtgga 501643DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 16ggtccaacgc cgggggcagc ggtagggata acagggtaat cca 431741DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 17tatggattac cctgttatcc ctaccgctgc ccccggcgtt g 411850DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 18gacctccact ccattttctg ggagtccaga atctgtctca ggctgcttgc 501945DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 19ctattttgca cccagctaca attttatcct gaatcttacc aacgc 452039DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 20ggcttagagc ttaattgctg aatctggtgc tgtagctca 392147DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 21ggggagctct gggggcagcg gtagggataa cccctcaggc tagatgc 472253DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 22tcgagcatct agcctgaggg gttatcccta ccgctgcccc cagagctccc cgc 532331DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 23atggtaaagc aagatgaagt tatcacattg t 312428DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 24atgtcaacta ttaaatgctt ggtttaag 282527DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 25gcgcctggtc tgtacaccgt tcatctg 272640DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 26ctcgagtcgg ccgcccatgg caacagtttc agcggagtga 402742DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 27ccatgggcgg ccgactcgag gaaagttgtt tagcaaaacc cc 422820DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 28catgttactt agccggaacg 202970DNAArtificial SequenceDescription of Artificial Sequence Synthetic oligonucleotide 29cgacctgctc tgcggccgcc gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac 60cgctgccccc 7030353PRTArtificial SequenceDescription of Artificial Sequence Synthetic polypeptide 30Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 1 5 10 15 Arg Gly Ser His Arg Ser Thr Leu Glu Asp Pro Ser Gln Ser Thr Asn 20 25 30 Val Leu Gly Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys

Ala 35 40 45 Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu 50 55 60 Asn Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 65 70 75 80 Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 85 90 95 Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 100 105 110 Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn 115 120 125 Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser 130 135 140 Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 145 150 155 160 Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe 165 170 175 Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly 180 185 190 Phe Ile Gln Ser Leu Lys Asp Asp Pro Arg Ser Thr Leu Ala Ala Ala 195 200 205 Ser Gly Ala Ser Thr Asp Thr Tyr Lys Leu Ile Leu Asn Gly Lys Thr 210 215 220 Leu Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu 225 230 235 240 Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp 245 250 255 Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Pro Glu 260 265 270 Val Ile Asp Ala Ser Glu Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu 275 280 285 Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Lys Ala Val 290 295 300 Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln Tyr Ala Asn Asp Asn 305 310 315 320 Gly Val Asp Gly Val Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr 325 330 335 Val Thr Glu Met Val Thr Glu Val Pro Val Val Arg Gly Gly Ser Cys 340 345 350 Gly 31470PRTArtificial SequenceDescription of Artificial Sequence Synthetic polypeptide 31Met Ser Ala Cys Lys Gly Met Gly Ser Ser His His His His His His 1 5 10 15 Ser Ser Gly Leu Val Pro Arg Gly Ser His Met Ala Arg Ile Val Asp 20 25 30 Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile 35 40 45 Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp 50 55 60 Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe 65 70 75 80 Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser 85 90 95 Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr 100 105 110 Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn 115 120 125 Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn 130 135 140 Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr 145 150 155 160 Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser 165 170 175 Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr 180 185 190 Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg 195 200 205 Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser 210 215 220 Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr 225 230 235 240 Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys 245 250 255 Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys 260 265 270 Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Leu 275 280 285 Asn Leu Tyr Asp Pro Asn Lys Tyr Val Asp Val Asn Asn Val Gly Ile 290 295 300 Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val Met Thr Thr 305 310 315 320 Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys Phe Ile Ile 325 330 335 Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg Asn Asn Asp 340 345 350 Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr Arg Leu Ala 355 360 365 Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser Ala Leu Glu 370 375 380 Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met Lys Ser Lys 385 390 395 400 Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu Gln Asp Asn 405 410 415 Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe His Gln Phe Asn Asn Ile 420 425 430 Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile Glu Arg Ser 435 440 445 Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val Asp Asp Gly 450 455 460 Trp Gly Glu Arg Pro Leu 465 470 324838DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 32agatctgcaa gtcttgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc 60ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg 120gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg 180cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg 240gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc 300tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac 360ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga 420tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg 480ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc 540gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc 600ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta 660agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga 720gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc 780ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga 840ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg 900gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg 960ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat 1020tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc 1080aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca 1140aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac 1200ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga 1260cgctttttat cgcaactctc tactgtttct ccatacccgt ttttttggat ggagtgaaac 1320gatggcgatt gcaatttcta gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 1380cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 1440cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 1500gaattcgagc tagaaataat tttgtttaac tttaagaagg agatatacca tggcaagcag 1560cctgagacag attctggact cccagaaaat ggagtggagg tccaacgccg ggggcagcgg 1620tagggataac agggtaatcc atatgctcga gggggcccag gcggccgcac tcgactcggt 1680acccggggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata 1740gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 1800cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 1860ctcactgccc gctttccagt cgggaaacct gtcgtgccag cgagctcgaa ttcggctgct 1920aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa 1980ccccttgggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg 2040gatcggagat caattctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 2100gttgcgtagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 2160tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2220cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 2280ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 2340ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 2400gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 2460tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 2520aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 2580ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 2640acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 2700aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 2760attttgccgt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 2820tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 2880gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 2940cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc 3000tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 3060agtaagagaa ttatgcagtg ctgccataag catgagtgat aacactgcgg ccaacttact 3120tctgacaacg atcggaggac cgaaggagct aaccgctttt tttcacaaca tgggggatca 3180tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 3240tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 3300acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 3360accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 3420tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 3480cgtagttatc tacacgacgg gcagtcaggc aactatggat gaacgaaata gacagatcgc 3540tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 3600actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 3660tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 3720cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 3780gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3840tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 3900gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3960gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4020ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4080acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg 4140agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4200cggaacagga gagcgcacga gggagcttcc aggggggaac gcctggtatc tttatagtcc 4260tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcc 4320gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4380ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 4440ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 4500cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 4560acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag 4620tatacactcc gctatcgcta cgtgactgca aggagatggc gcccaacagt cccccggcca 4680cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc 4740gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg 4800tgatgccggc cacgatgcgt ccggcgtaga ggatcttg 48383340345DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 33gggcggcgac ctcgcgggtt ttcgctattt atgaaaattt tccggtttaa ggcgtttccg 60ttcttcttcg tcataactta atgtttttat ttaaaatacc ctctgaaaag aaaggaaacg 120acaggtgctg aaagcgaggc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt 180ggaatgaaca atggaagtca acaaaaagca gctggctgac attttcggtg cgagtatccg 240taccattcag aactggcagg aacagggaat gcccgttctg cgaggcggtg gcaagggtaa 300tgaggtgctt tatgactctg ccgccgtcat aaaatggtat gccgaaaggg atgctgaaat 360tgagaacgaa aagctgcgcc gggaggttga agaactgcgg caggccagcg aggcagatct 420ccagccagga actattgagt acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca 480ggaactgaag aatgccagag actccgctga agtggtggaa accgcattct gtactttcgt 540gctgtcgcgg atcgcaggtg aaattgccag tattctcgac gggctccccc tgtcggtgca 600gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa 660agccatgaac aaagcagccg cgctggatga actgataccg gggttgctga gtgaatatat 720cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca 780ggccggagcc acagaccgcc gttgaatggg cggatgctaa ttactatctc ccgaaagaat 840ccgcatacca ggaagggcgc tgggaaacac tgccctttca gcgggccatc atgaatgcga 900tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca 960aaatgctgct gggtgtttat gcctacttta tagagcataa gcagcgcaac acccttatct 1020ggttgccgac ggatggtgat gccgagaact ttatgaaaac ccacgttgag ccgactattc 1080gtgatattcc gtcgctgctg gcgctggccc cgtggtatgg caaaaagcac cgggataaca 1140cgctcaccat gaagcgtttc actaatgggc gtggcttctg gtgcctgggc ggtaaagcgg 1200caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta tgatgaactt gctgcttttg 1260atgatgatat tgaacaggaa ggctctccga cgttcctggg tgacaagcgt attgaaggct 1320cggtctggcc aaagtccatc cgtggctcca cgccaaaagt gagaggcacc tgtcagattg 1380agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca tgttgcctgc ccgcattgcg 1440gggaggagca gtatcttaaa tttggcgaca aagagacgcc gtttggcctc aaatggacgc 1500cggatgaccc ctccagcgtg ttttatctct gcgagcataa tgcctgcgtc atccgccagc 1560aggagctgga ctttactgat gcccgttata tctgcgaaaa gaccgggatc tggacccgtg 1620atggcattct ctggttttcg tcatccggtg aagagattga gccacctgac agtgtgacct 1680ttcacatctg gacagcgtac agcccgttca ccacctgggt gcagattgtc aaagactgga 1740tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga 1800cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt gatggcagag cggaaagagc 1860attattcagc gcccgttcct gaccgtgtgg cttacctgac cgccggtatc gactcccagc 1920tggaccgcta cgaaatgcgc gtatggggat gggggccggg tgaggaaagc tggctgattg 1980accggcagat tattatgggc cgccacgacg atgaacagac gctgctgcgt gtggatgagg 2040ccatcaataa aacctatacc cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct 2100gggatactgg cgggattgac ccgaccattg tgtatgaacg ctcgaaaaaa catgggctgt 2160tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccggtggcc agcatgccac 2220gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc gcgaaagagc 2280agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc ggtgccgttc 2340acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg actgctgaag 2400agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc aaaaagcgac 2460gcaatgaggc actcgactgc ttcgtttatg cgctggcggc gctgcgcatc agtatttccc 2520gctggcagct ggatctcagt gcgctgctgg cgagcctgca ggaagaggat ggtgcagcaa 2580ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat gaatgacgcg 2640acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag gtaaacgggt 2700ggcaacagta cagaaagacg gacgaagggt ggagtttacg gccacttccg tgtctgacct 2760gaaaaaatat attgcagagc tggaagtgca gaccggcatg acacagcgac gcaggggacc 2820tgcaggattt tatgtatgaa aacgcccacc attcccaccc ttctggggcc ggacggcatg 2880acatcgctgc gcgaatatgc cggttatcac ggcggtggca gcggatttgg agggcagttg 2940cggtcgtgga acccaccgag tgaaagtgtg gatgcagccc tgttgcccaa ctttacccgt 3000ggcaatgccc gcgcagacga tctggtacgc aataacggct atgccgccaa cgccatccag 3060ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc 3120tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg 3180aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc 3240atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc 3300acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag 3360cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt 3420aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg 3480ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcctc gttcattcac 3540gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta cagcgtgatg 3600gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc cattgtgaag 3660gcgatgtatg ccgccaccat tgagagtgag ctggatacgc agtcagcgat ggattttatt 3720ctgggcgcga acagtcagga gcagcgggaa aggctgaccg gctggattgg tgaaattgcc 3780gcgtattacg ccgcagcgcc ggtccggctg ggaggcgcaa aagtaccgca cctgatgccg 3840ggtgactcac tgaacctgca gacggctcag gatacggata acggctactc cgtgtttgag 3900cagtcactgc tgcggtatat cgctgccggg ctgggtgtct cgtatgagca gctttcccgg 3960aattacgccc agatgagcta ctccacggca cgggccagtg cgaacgagtc gtgggcgtac 4020tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg 4080ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt caaaagcgcg cttcagtttt 4140caggaagccc gcagtgcctg ggggaactgc gactggatag gctccggtcg tatggccatc 4200gatggtctga aagaagttca ggaagcggtg atgctgatag aagccggact gagtacctac 4260gagaaagagt gcgcaaaacg cggtgacgac tatcaggaaa tttttgccca gcaggtccgt 4320gaaacgatgg agcgccgtgc agccggtctt aaaccgcccg cctgggcggc tgcagcattt 4380gaatccgggc tgcgacaatc aacagaggag gagaagagtg acagcagagc tgcgtaatct 4440cccgcatatt gccagcatgg cctttaatga gccgctgatg cttgaacccg cctatgcgcg 4500ggttttcttt tgtgcgcttg caggccagct tgggatcagc agcctgacgg atgcggtgtc 4560cggcgacagc ctgactgccc aggaggcact cgcgacgctg gcattatccg gtgatgatga 4620cggaccacga caggcccgca gttatcaggt catgaacggc atcgccgtgc tgccggtgtc 4680cggcacgctg gtcagccgga cgcgggcgct gcagccgtac tcggggatga ccggttacaa 4740cggcattatc gcccgtctgc aacaggctgc cagcgatccg atggtggacg gcattctgct 4800cgatatggac acgcccggcg ggatggtggc gggggcattt gactgcgctg acatcatcgc

4860ccgtgtgcgt gacataaaac cggtatgggc gcttgccaac gacatgaact gcagtgcagg 4920tcagttgctt gccagtgccg cctcccggcg tctggtcacg cagaccgccc ggacaggctc 4980catcggcgtc atgatggctc acagtaatta cggtgctgcg ctggagaaac agggtgtgga 5040aatcacgctg atttacagcg gcagccataa ggtggatggc aacccctaca gccatcttcc 5100ggatgacgtc cgggagacac tgcagtcccg gatggacgca acccgccaga tgtttgcgca 5160gaaggtgtcg gcatataccg gcctgtccgt gcaggttgtg ctggataccg aggctgcagt 5220gtacagcggt caggaggcca ttgatgccgg actggctgat gaacttgtta acagcaccga 5280tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa tcccgtctct caggagggcg 5340aatgaccaaa gagactcaat caacaactgt ttcagccact gcttcgcagg ctgacgttac 5400tgacgtggtg ccagcgacgg agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc 5460gcagatcacc gcagcggttg cggcagaaaa cagccgcatt atggggatcc tcaactgtga 5520ggaggctcac ggacgcgaag aacaggcacg cgtgctggca gaaacccccg gtatgaccgt 5580gaaaacggcc cgccgcattc tggccgcagc accacagagt gcacaggcgc gcagtgacac 5640tgcgctggat cgtctgatgc agggggcacc ggcaccgctg gctgcaggta acccggcatc 5700tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg atgtttatga cgagcaaaga 5760aacctttacc cattaccagc cgcagggcaa cagtgacccg gctcataccg caaccgcgcc 5820cctagacctt catcactaaa ggccgcctgt gcggcttttt ttacgggatt tttttatgtc 5880gatgtacaca accgcccaac tgctggcggc aaatgagcag aaatttaagt ttgatccgct 5940gtttctgcgt ctctttttcc gtgagagcta tcccttcacc acggagaaag tctatctctc 6000acaaattccg ggactggtaa acatggcgct gtacgtttcg ccgattgttt ccggtgaggt 6060tatccgttcc cgtggcggct ccacctctga atttacgccg ggatatgtca agccgaagca 6120tgaagtgaat ccgcagatga ccctgcgtcg cctgccggat gaagatccgc agaatctggc 6180ggacccggct taccgccgcc gtcgcatcat catgcagaac atgcgtgacg aagagctggc 6240cattgctcag gtcgaagaga tgcaggcagt ttctgccgtg cttaagggca aatacaccat 6300gaccggtgaa gccttcgatc cggttgaggt ggatatgggc cgcagtgagg agaataacat 6360cacgcagtcc ggcggcacgg agtggagcaa gcgtgacaag tccacgtatg acccgaccga 6420cgatatcgaa gcctacgcgc tgaacgccag cggtgtggtg aatatcatcg tgttcgatcc 6480gaaaggctgg gcgctgttcc gttccttcaa agccgtcaag gagaagctgg atacccgtcg 6540tggctctaat tccgagctgg agacagcggt gaaagacctg ggcaaagcgg tgtcctataa 6600ggggatgtat ggcgatgtgg ccatcgtcgt gtattccgga cagtacgtgg aaaacggcgt 6660caaaaagaac ttcctgccgg acaacacgat ggtgctgggg aacactcagg cacgcggtct 6720gcgcacctat ggctgcattc aggatgcgga cgcacagcgc gaaggcatta acgcctctgc 6780ccgttacccg aaaaactggg tgaccaccgg cgatccggcg cgtgagttca ccatgattca 6840gtcagcaccg ctgatgctgc tggctgaccc tgatgagttc gtgtccgtac aactggcgta 6900atcatggccc ttcggggcca ttgtttctct gtggaggagt ccatgacgaa agatgaactg 6960attgcccgtc tccgctcgct gggtgaacaa ctgaaccgtg atgtcagcct gacggggacg 7020aaagaagaac tggcgctccg tgtggcagag ctgaaagagg agcttgatga cacggatgaa 7080actgccggtc aggacacccc tctcagccgg gaaaatgtgc tgaccggaca tgaaaatgag 7140gtgggatcag cgcagccgga taccgtgatt ctggatacgt ctgaactggt cacggtcgtg 7200gcactggtga agctgcatac tgatgcactt cacgccacgc gggatgaacc tgtggcattt 7260gtgctgccgg gaacggcgtt tcgtgtctct gccggtgtgg cagccgaaat gacagagcgc 7320ggcctggcca gaatgcaata acgggaggcg ctgtggctga tttcgataac ctgttcgatg 7380ctgccattgc ccgcgccgat gaaacgatac gcgggtacat gggaacgtca gccaccatta 7440catccggtga gcagtcaggt gcggtgatac gtggtgtttt tgatgaccct gaaaatatca 7500gctatgccgg acagggcgtg cgcgttgaag gctccagccc gtccctgttt gtccggactg 7560atgaggtgcg gcagctgcgg cgtggagaca cgctgaccat cggtgaggaa aatttctggg 7620tagatcgggt ttcgccggat gatggcggaa gttgtcatct ctggcttgga cggggcgtac 7680cgcctgccgt taaccgtcgc cgctgaaagg gggatgtatg gccataaaag gtcttgagca 7740ggccgttgaa aacctcagcc gtatcagcaa aacggcggtg cctggtgccg ccgcaatggc 7800cattaaccgc gttgcttcat ccgcgatatc gcagtcggcg tcacaggttg cccgtgagac 7860aaaggtacgc cggaaactgg taaaggaaag ggccaggctg aaaagggcca cggtcaaaaa 7920tccgcaggcc agaatcaaag ttaaccgggg ggatttgccc gtaatcaagc tgggtaatgc 7980gcgggttgtc ctttcgcgcc gcaggcgtcg taaaaagggg cagcgttcat ccctgaaagg 8040tggcggcagc gtgcttgtgg tgggtaaccg tcgtattccc ggcgcgttta ttcagcaact 8100gaaaaatggc cggtggcatg tcatgcagcg tgtggctggg aaaaaccgtt accccattga 8160tgtggtgaaa atcccgatgg cggtgccgct gaccacggcg tttaaacaaa atattgagcg 8220gatacggcgt gaacgtcttc cgaaagagct gggctatgcg ctgcagcatc aactgaggat 8280ggtaataaag cgatgaaaca tactgaactc cgtgcagccg tactggatgc actggagaag 8340catgacaccg gggcgacgtt ttttgatggt cgccccgctg tttttgatga ggcggatttt 8400ccggcagttg ccgtttatct caccggcgct gaatacacgg gcgaagagct ggacagcgat 8460acctggcagg cggagctgca tatcgaagtt ttcctgcctg ctcaggtgcc ggattcagag 8520ctggatgcgt ggatggagtc ccggatttat ccggtgatga gcgatatccc ggcactgtca 8580gatttgatca ccagtatggt ggccagcggc tatgactacc ggcgcgacga tgatgcgggc 8640ttgtggagtt cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc 8700tgtaccaaat cctacaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg 8760gagcggtgac ccttacgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt 8820taaagacctg acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga 8880tgaagatgca gactggactg cgaccgggca ggggcagaaa tctgccggag ataccagctt 8940cacgctggcg tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga 9000aggcgatacc cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg 9060ctgggtcagc agtatcggta aggcggtgac ggcgaaggaa gtgatcaccc gcacggtgaa 9120agtcaccaat gtgggacgtc cgtcgatggc agaagatcgc agcacggtaa cagcggcaac 9180cggcatgacc gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac 9240cgtggccttc cagccggagg gcgtaaccga caagagcttt cgtgcggtgt ctgcggataa 9300aacaaaagcc accgtgtcgg tcagtggtat gaccatcacc gtgaacggcg ttgctgcagg 9360caaggtcaac attccggttg tatccggtaa tggtgagttt gctgcggttg cagaaattac 9420cgtcaccgcc agttaatccg gagagtcagc gatgttcctg aaaaccgaat catttgaaca 9480taacggtgtg accgtcacgc tttctgaact gtcagccctg cagcgcattg agcatctcgc 9540cctgatgaaa cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttactgtgga 9600agacgccatc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccgca 9660gaagacgcag atgccgtcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac 9720cacctggccc acggaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtctggtat 9780gtatgagttt gtggtgaata atgcccctga acagacagag gacgccgggc ccgcagagcc 9840tgtttctgcg ggaaagtgtt cgacggtgag ctgagttttg ccctgaaact ggcgcgtgag 9900atggggcgac ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac 9960tggcaccgct tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc 10020gggctgacgt acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat 10080ttcagtctgc tgaaccggcg cgaggctgac gaagagcctg aagatgatgt gctgatgcag 10140aaagcggcag ggcttgccgg aggtgtccgc tttggcccgg acgggaatga agttatcccc 10200gcttccccgg atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa 10260gggatcgcag gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttgag 10320tctggatgcg gccagatttg acgagcagat ggccagagtc aggcgtcatt tttctggtac 10380ggaaagtgat gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct 10440ggctgcacag aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc 10500tgcacagttc accgacgtgg ccacgcagct tgcaggcggg caaagtccgt ggctgatcct 10560gctgcaacag ggggggcagg tgaaggactc cttcggcggg atgatcccca tgttcagggg 10620gcttgccggt gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg 10680tgcgctggcg tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct 10740ggtcctttcc ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc 10800cgggcaggcg gcagggctga cgtttaacca gaccagcgag tcactcagcg cactggttaa 10860ggcgggggta agcggtgagg ctcagattgc gtccatcagc cagagtgtgg cgcgtttctc 10920ctctgcatcc ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga 10980cccgacgtcg gggctgacgg cgatggctcg ccagttccat aacgtgtcgg cggagcagat 11040tgcgtatgtt gctcagttgc agcgttccgg cgatgaagcc ggggcattgc aggcggcgaa 11100cgaggccgca acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac 11160gctggagacc tgggcagaca ggactgcgcg ggcattcaaa tccatgtggg atgcggtgct 11220ggatattggt cgtcctgata ccgcgcagga gatgctgatt aaggcagagg ctgcgtataa 11280gaaagcagac gacatctgga atctgcgcaa ggatgattat tttgttaacg atgaagcgcg 11340ggcgcgttac tgggatgatc gtgaaaaggc ccgtcttgcg cttgaagccg cccgaaagaa 11400ggctgagcag cagactcaac aggacaaaaa tgcgcagcag cagagcgata ccgaagcgtc 11460acggctgaaa tataccgaag aggcgcagaa ggcttacgaa cggctgcaga cgccgctgga 11520gaaatatacc gcccgtcagg aagaactgaa caaggcactg aaagacggga aaatcctgca 11580ggcggattac aacacgctga tggcggcggc gaaaaaggat tatgaagcga cgctgaaaaa 11640gccgaaacag tccagcgtga aggtgtctgc gggcgatcgt caggaagaca gtgctcatgc 11700tgccctgctg acgcttcagg cagaactccg gacgctggag aagcatgccg gagcaaatga 11760gaaaatcagc cagcagcgcc gggatttgtg gaaggcggag agtcagttcg cggtactgga 11820ggaggcggcg caacgtcgcc agctgtctgc acaggagaaa tccctgctgg cgcataaaga 11880tgagacgctg gagtacaaac gccagctggc tgcacttggc gacaaggtta cgtatcagga 11940gcgcctgaac gcgctggcgc agcaggcgga taaattcgca cagcagcaac gggcaaaacg 12000ggccgccatt gatgcgaaaa gccgggggct gactgaccgg caggcagaac gggaagccac 12060ggaacagcgc ctgaaggaac agtatggcga taatccgctg gcgctgaata acgtcatgtc 12120agagcagaaa aagacctggg cggctgaaga ccagcttcgc gggaactgga tggcaggcct 12180gaagtccggc tggagtgagt gggaagagag cgccacggac agtatgtcgc aggtaaaaag 12240tgcagccacg cagacctttg atggtattgc acagaatatg gcggcgatgc tgaccggcag 12300tgagcagaac tggcgcagct tcacccgttc cgtgctgtcc atgatgacag aaattctgct 12360taagcaggca atggtgggga ttgtcgggag tatcggcagc gccattggcg gggctgttgg 12420tggcggcgca tccgcgtcag gcggtacagc cattcaggcc gctgcggcga aattccattt 12480tgcaaccgga ggatttacgg gaaccggcgg caaatatgag ccagcgggga ttgttcaccg 12540tggtgagttt gtcttcacga aggaggcaac cagccggatt ggcgtgggga atctttaccg 12600gctgatgcgc ggctatgcca ccggcggtta tgtcggtaca ccgggcagca tggcagacag 12660ccggtcgcag gcgtccggga cgtttgagca gaataaccat gtggtgatta acaacgacgg 12720cacgaacggg cagataggtc cggctgctct gaaggcggtg tatgacatgg cccgcaaggg 12780tgcccgtgat gaaattcaga cacagatgcg tgatggtggc ctgttctccg gaggtggacg 12840atgaagacct tccgctggaa agtgaaaccc ggtatggatg tggcttcggt cccttctgta 12900agaaaggtgc gctttggtga tggctattct cagcgagcgc ctgccgggct gaatgccaac 12960ctgaaaacgt acagcgtgac gctttctgtc ccccgtgagg aggccacggt actggagtcg 13020tttctggaag agcacggggg ctggaaatcc tttctgtgga cgccgcctta tgagtggcgg 13080cagataaagg tgacctgcgc aaaatggtcg tcgcgggtca gtatgctgcg tgttgagttc 13140agcgcagagt ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg 13200aatgcacccg tgcggagcag tcggccagcg tggtgctctg ggaaatcgac ctgacagagg 13260tcggtggaga acgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct 13320ggcaggggcg acagtatcag ccgtatccca ttcaggggag cggttttgaa ctgaatggca 13380aaggcaccag tacgcgcccc acgctgacgg tttctaacct gtacggtatg gtcaccggga 13440tggcggaaga tatgcagagt ctggtcggcg gaacggtggt ccggcgtaag gtttacgccc 13500gttttctgga tgcggtgaac ttcgtcaacg gaaacagtta cgccgatccg gagcaggagg 13560tgatcagccg ctggcgcatt gagcagtgca gcgaactgag cgcggtgagt gcctcctttg 13620tactgtccac gccgacggaa acggatggcg ctgtttttcc gggacgtatc atgctggcca 13680acacctgcac ctggacctat cgcggtgacg agtgcggtta tagcggtccg gctgtcgcgg 13740atgaatatga ccagccaacg tccgatatca cgaaggataa atgcagcaaa tgcctgagcg 13800gttgtaagtt ccgcaataac gtcggcaact ttggcggctt cctttccatt aacaaacttt 13860cgcagtaaat cccatgacac agacagaatc agcgattctg gcgcacgccc ggcgatgtgc 13920gccagcggag tcgtgcggct tcgtggtaag cacgccggag ggggaaagat atttcccctg 13980cgtgaatatc tccggtgagc cggaggctat ttccgtatgt cgccggaaga ctggctgcag 14040gcagaaatgc agggtgagat tgtggcgctg gtccacagcc accccggtgg tctgccctgg 14100ctgagtgagg ccgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc 14160cgggggacga ttcataagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac 14220ggtgtgacgg actgttacac actgttccgg gatgcttatc atctggcggg gattgagatg 14280ccggactttc atcgtgagga tgactggtgg cgtaacggcc agaatctcta tctggataat 14340ctggaggcga cggggctgta tcaggtgccg ttgtcagcgg cacagccggg cgatgtgctg 14400ctgtgctgtt ttggttcatc agtgccgaat cacgccgcaa tttactgcgg cgacggcgag 14460ctgctgcacc atattcctga acaactgagc aaacgagaga ggtacaccga caaatggcag 14520cgacgcacac actccctctg gcgtcaccgg gcatggcgcg catctgcctt tacggggatt 14580tacaacgatt tggtcgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccggg 14640cactggccac acagctcccg gcgtttcgtc agaaactgag cgacggctgg tatcaggtac 14700ggattgccgg gcgggacgtc agcacgtccg ggttaacggc gcagttacat gagactctgc 14760ctgatggcgc tgtaattcat attgttccca gagtcgccgg ggccaagtca ggtggcgtat 14820tccagattgt cctgggggct gccgccattg ccggatcatt ctttaccgcc ggagccaccc 14880ttgcagcatg gggggcagcc attggggccg gtggtatgac cggcatcctg ttttctctcg 14940gtgccagtat ggtgctcggt ggtgtggcgc agatgctggc accgaaagcc agaactcccc 15000gtatacagac aacggataac ggtaagcaga acacctattt ctcctcactg gataacatgg 15060ttgcccaggg caatgttctg cctgttctgt acggggaaat gcgcgtgggg tcacgcgtgg 15120tttctcagga gatcagcacg gcagacgaag gggacggtgg tcaggttgtg gtgattggtc 15180gctgatgcaa aatgttttat gtgaaaccgc ctgcgggcgg ttttgtcatt tatggagcgt 15240gaggaatggg taaaggaagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga 15300agtccacgca gttgctgagt gtgatcgatg ccatcagcga agggccgatt gaaggtccgg 15360tggatggctt aaaaagcgtg ctgctgaaca gtacgccggt gctggacact gaggggaata 15420ccaacatatc cggtgtcacg gtggtgttcc gggctggtga gcaggagcag actccgccgg 15480agggatttga atcctccggc tccgagacgg tgctgggtac ggaagtgaaa tatgacacgc 15540cgatcacccg caccattacg tctgcaaaca tcgaccgtct gcgctttacc ttcggtgtac 15600aggcactggt ggaaaccacc tcaaagggtg acaggaatcc gtcggaagtc cgcctgctgg 15660ttcagataca acgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa 15720ccacctcgca gtatctggcc tcggtggtga tgggtaacct gccgccgcgc ccgtttaata 15780tccggatgcg caggatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct 15840ggtcgtcata cactgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg 15900gcgtgcaggt ggactcggag cagttcggca gccagcaggt gagccgtaat tatcatctgc 15960gcgggcgtat tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta 16020tctgggacgg aacgtttaaa ccggcataca gcaacaacat ggcctggtgt ctgtgggata 16080tgctgaccca tccgcgctac ggcatgggga aacgtcttgg tgcggcggat gtggataaat 16140gggcgctgta tgtcatcggc cagtactgcg accagtcagt gccggacggc tttggcggca 16200cggagccgcg catcacctgt aatgcgtacc tgaccacaca gcgtaaggcg tgggatgtgc 16260tcagcgattt ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt 16320tcgtgcagga ccgaccgtcg gataagacgt ggacctataa ccgcagtaat gtggtgatgc 16380cggatgatgg cgcgccgttc cgctacagct tcagcgccct gaaggaccgc cataatgccg 16440ttgaggtgaa ctggattgac ccgaacaacg gctgggagac ggcgacagag cttgttgaag 16500atacgcaggc cattgcccgt tacggtcgta atgttacgaa gatggatgcc tttggctgta 16560ccagccgggg gcaggcacac cgcgccgggc tgtggctgat taaaacagaa ctgctggaaa 16620cgcagaccgt ggatttcagc gtcggcgcag aagggcttcg ccatgtaccg ggcgatgtta 16680ttgaaatctg cgatgatgac tatgccggta tcagcaccgg tggtcgtgtg ctggcggtga 16740acagccagac ccggacgctg acgctcgacc gtgaaatcac gctgccatcc tccggtaccg 16800cgctgataag cctggttgac ggaagtggca atccggtcag cgtggaggtt cagtccgtca 16860ccgacggcgt gaaggtaaaa gtgagccgtg ttcctgacgg tgttgctgaa tacagcgtat 16920gggagctgaa gctgccgacg ctgcgccagc gactgttccg ctgcgtgagt atccgtgaga 16980acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg 17040tggataacgg ggcgcacttt gacggcgaac agagtggcac ggtgaatggt gtcacgccgc 17100cagcggtgca gcacctgacc gcagaagtca ctgcagacag cggggaatat caggtgctgg 17160cgcgatggga cacaccgaag gtggtgaagg gcgtgagttt cctgctccgt ctgaccgtaa 17220cagcggacga cggcagtgag cggctggtca gcacggcccg gacgacggaa accacatacc 17280gcttcacgca actggcgctg gggaactaca ggctgacagt ccgggcggta aatgcgtggg 17340ggcagcaggg cgatccggcg tcggtatcgt tccggattgc cgcaccggca gcaccgtcga 17400ggattgagct gacgccgggc tattttcaga taaccgccac gccgcatctt gccgtttatg 17460acccgacggt acagtttgag ttctggttct cggaaaagca gattgcggat atcagacagg 17520ttgaaaccag cacgcgttat cttggtacgg cgctgtactg gatagccgcc agtatcaata 17580tcaaaccggg ccatgattat tacttttata tccgcagtgt gaacaccgtt ggcaaatcgg 17640cattcgtgga ggccgtcggt cgggcgagcg atgatgcgga aggttacctg gattttttca 17700aaggcaagat aaccgaatcc catctcggca aggagctgct ggaaaaagtc gagctgacgg 17760aggataacgc cagcagactg gaggagtttt cgaaagagtg gaaggatgcc agtgataagt 17820ggaatgccat gtgggctgtc aaaattgagc agaccaaaga cggcaaacat tatgtcgcgg 17880gtattggcct cagcatggag gacacggagg aaggcaaact gagccagttt ctggttgccg 17940ccaatcgtat cgcatttatt gacccggcaa acgggaatga aacgccgatg tttgtggcgc 18000agggcaacca gatattcatg aacgacgtgt tcctgaagcg cctgacggcc cccaccatta 18060ccagcggcgg caatcctccg gccttttccc tgacaccgga cggaaagctg accgctaaaa 18120atgcggatat cagtggcagt gtgaatgcga actccgggac gctcagtaat gtgacgatag 18180ctgaaaactg tacgataaac ggtacgctga gggcggaaaa aatcgtcggg gacattgtaa 18240aggcggcgag cgcggctttt ccgcgccagc gtgaaagcag tgtggactgg ccgtcaggta 18300cccgtactgt caccgtgacc gatgaccatc cttttgatcg ccagatagtg gtgcttccgc 18360tgacgtttcg cggaagtaag cgtactgtca gcggcaggac aacgtattcg atgtgttatc 18420tgaaagtact gatgaacggt gcggtgattt atgatggcgc ggcgaacgag gcggtacagg 18480tgttctcccg tattgttgac atgccagcgg gtcggggaaa cgtgatcctg acgttcacgc 18540ttacgtccac acggcattcg gcagatattc cgccgtatac gtttgccagc gatgtgcagg 18600ttatggtgat taagaaacag gcgctgggca tcagcgtggt ctgagtgtgt tacagaggtt 18660cgtccgggaa cgggcgtttt attataaaac agtgagaggt gaacgatgcg taatgtgtgt 18720attgccgttg ctgtctttgc cgcacttgcg gtgacagtca ctccggcccg tgcggaaggt 18780ggacatggta cgtttacggt gggctatttt caagtgaaac cgggtacatt gccgtcgttg 18840tcgggcgggg ataccggtgt gagtcatctg aaagggatta acgtgaagta ccgttatgag 18900ctgacggaca gtgtgggggt gatggcttcc ctggggttcg ccgcgtcgaa aaagagcagc 18960acagtgatga ccggggagga tacgtttcac tatgagagcc tgcgtggacg ttatgtgagc 19020gtgatggccg gaccggtttt acaaatcagt aagcaggtca gtgcgtacgc catggccgga 19080gtggctcaca gtcggtggtc cggcagtaca atggattacc gtaagacgga aatcactccc 19140gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt 19200ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 19260agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 19320tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 19380cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 19440gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 19500ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 19560ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 19620atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 19680aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 19740gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 19800ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 19860ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac

19920acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 19980gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 20040ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 20100ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 20160gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 20220ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 20280agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 20340ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 20400gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 20460catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 20520cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 20580cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 20640gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 20700tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 20760gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 20820tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 20880gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 20940gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 21000taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 21060tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 21120ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 21180taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 21240tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 21300aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta 21360ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt 21420tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 21480tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 21540gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc 21600ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat 21660tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 21720tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 21780cacgacgttg taaaacgacg gccagtgaat tcgattttaa gatacattga tgagtttgga 21840caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 21900gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat 21960tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac 22020aaatgtggta tggctgatta tgatcagtta tctagagtcg cggccgcttt acttgtacag 22080ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc 22140gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg 22200cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac 22260gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt 22320gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat 22380gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc 22440ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg 22500ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc 22560ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg 22620cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc 22680gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac 22740caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg 22800tggatcgatc ctagcggatc tgacggttca ctaaaccagc tctgcttata tagacctccc 22860accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa 22920agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg 22980gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca 23040ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg 23100tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg 23160tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca 23220cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg 23280acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg 23340taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaaa 23400tcactagtga attcgattaa agcgacggca cagctcgcgg aaaatatcaa agtcgttgcg 23460cgcctcgaac tgcggcggca ccacctgttt catggcgata atgccacggt tggagtggtt 23520gccgtactgg tcgagatcgt tacgctcaaa ctgcgtggtc gcaggcagca cgatatcggc 23580aaagcggcag gttgaggtcc actggttatc tatggcgata accgtttcca gcttgcgcca 23640gccttcaata atgcggttga tctgctgatg gcgatggaat gggttagttc cggcaaaaat 23700acacattttc agcggcggca gttttaccga tttaccgttc cagttgatca ctttccccgg 23760ttcgaggatc gcatcgataa aacgggcaat cggaatggtg ctgctgtagc ctttgtaatc 23820actgttgtcg tgaacaggcg gaatcgacgt agagccggag aaaccactca gaataacgcc 23880tttacgcccc ggcgtgcctg cgccgttata gtgccagcca aaaccaaagc caccacctgg 23940caggccaatt tgccccagca tcgccgccag aaccacaatc atccacgccc actgttcacc 24000gtgctgcata cgctgcacgc accagccagc aataatttgc gttctgttcg ccgccatctg 24060ccgcgccagc ccacgaatgg tttcggcatc aatgccggtc agtttttcag cccatgcggc 24120atctttcggc tgaccgtctt tctcacccag caggtacggc aggaactgct caaaacccac 24180acagtagtta gcgaggaagt ttttgtcgta caggttttca ctgtacagcg tatacgccag 24240cgccagttgc agcggcacat cagtttgcgg gttaaccgca atgtgcttca catgctcgcg 24300ccccagatac tcatgggtgg atgtgacaac cggatcgatg ctgatgacct caatttcacc 24360ggcggtgact ttcgctttta gctgcgcgta atattcataa acatcgtgat ccgggcacca 24420ccagttcgct tgctggtttt tcagcaaatc agagccccac agcacaatgg ttttgctgtt 24480ctgcaatacc agcggccagg aggtttgctg ttcatacact tccattgagc caaccacgcg 24540cggcaggatc acctgcgcag caccggtaga gtaatctccg cccgtaccaa cgctattacc 24600atgcaaggca atagctttcg ccagcatccc cgaagcgtta tggaacatcc ccgtcgattg 24660ccaaccactg gcggtcagca aggcactcgg cccgtgagtt ttctgcacgc gttccagttc 24720ttcatagaac atgtcgaggg cttcatccca gctcacgcgc acaaaacggt tatcaccgcg 24780ctgggaggta tcgctgagat ggcgcttacg cagccagtcc acgcgtacca tcggataacg 24840aatacgcgcc gcgttgtgta cgtgatccgg caatccggca atcattttcg acggatattt 24900atccagttcg aacggttttg ccgccacaaa gcgaccatcc ttcaccgtcg cgcggatagc 24960cccccagtgc gacccggtaa gaatgccctc tttcgagatg acagcctcag tcgccgcttg 25020cgccgcagtc gcacggcgcg gcgttaacaa tgacggcccc agcatcccgg cgacggttaa 25080gccgccgagt tgtgccagaa aacgccgacg tgatgcctga aagagatcgt tattgttcat 25140tatttttctt ccttcttatc gccgtgagcc ttacctgcgg tgtcagacgc attcatttgc 25200agatatttca acaaagtgcg ttcttcacgt ttatcgagac tggtaaagcc aatcatgccg 25260ttgagcgtgc cgatccaacc gttagcgtca aagtgggcga tttccggtgc gccgtggcac 25320tggttacagg tgccgttgta caacgaatcc gcataagccc agatcggttt gatatcgttc 25380accatgtcgc ctttcttcat ccacgcagtg gcctgcaact tgctccactc ggtattggtg 25440tcggcaacgg tggttttctc cagcgttttt acctgctgct gcacatcacc acgaatcgag 25500gcaacaaaga tgcgtttacc tgggaattgg gtgagtacac gctgacgtcc ggcgctttcc 25560gtccagccgg taatttcaat ttgcagccag tcgccgtcac gtttaaggac tttcacttcc 25620gaagcaggca gcagagaacc agaggcttct ttatcgcctt tcgccgcata aattggctta 25680atatcaatag agtacagcgt gtcaccactg tcattagcac tggcgcgcag ctcatcgaac 25740tgcttacgga agccgctact catatccggt aactggtggg caataccttt atgacagtcg 25800atgcaggatt gattatcttt cgctgccacc ttcatctgac gtgccgcttc aggatgctgc 25860ttcgcatgat ccatcgcatc gtagttatgg caggagcggc aggttgccga gttgttttct 25920ttcattcgcg cccattcacg ctcggcaagt tccgcgcgtt tggcttcgaa tttttcaggt 25980gtatcaatgg agtgagcaat aaaggtctgg tagatatcat tgctcgcttc cagtttgcgc 26040ttcaccatgc ctggaatatc cggcgggata tgacagtcat ggcattcagc tcgcacgccg 26100gaggcgttct ggaaatgcac cgactgttta tattcttcat acaccggttg catactgtgg 26160caactgacac aaaattcggt tgtgctggtg actttgatcc caacgtgtgg caatacaatc 26220agcgcaatgc caatcacaat cccaattgcg accagcgcca gtaccgacca acgagcactg 26280ggtcggcgta gcgcgttcca gagtttccgc ataatagccc ctgtaaaatt atggtttagt 26340gaagcgatct taatgagcaa atatgaacag cggcactggt caggatgaac ggcttacggc 26400agaatatgaa cagatatgaa cagaatgagt aaaaccctct gatgccacat cacattgtta 26460ttgttgaaga tgagccggtt acccaggcgc gattacaatc ttacttcact caggaggggt 26520ataccgtttc cgttacagcg agcggtgccg ggctgcggga aattatgcag aatcagccgg 26580tagatttaat tctgctggat atcaacttac ccgatgaaaa tggcctgatg ttaacccgcg 26640ccctgcgaga acgctcaacg gtggggatta ttctggttac cggacgcagc gatcggattg 26700accgtattgt tgggctggaa atgggcgcag acgattacgt caccaaaccg ctggaactgc 26760gcgaactggt agtacgggtg aaaaatctgc tctggcgaat cgacctcgcg cgacaagctc 26820aaccgcacac tcaggacaac tgctatcgct ttgccggtta ttgcctgaat gtgtcgcgcc 26880atacgctgga gcgggatggc gagccgatta aactgacccg cgcagagtat gaaatgttgg 26940tggcatttgt gacgaatccg ggcgaaattc tcagccgtga acgtctgcta cgtatgcttt 27000ctgcgcgtcg ggtggaaaac cctgacctgc gcaccgtcga tgtgttaatt cgtcgtttac 27060gtcataaact cagcgcggat ttactggtga cgcaacatgg tgaaggttat ttcttagccg 27120ctgatgtgtg ctgataaaaa tagaccggac gaaatccccc tggtgacagc gagcggcgga 27180tatgttctcg gtcggcattt ttcggcgtca gaactaaaat cggtgggctg acattatcag 27240acaccgattg cccctgtaat tgcctgatgg cctgctcaac tgccagttcc ccctgccaga 27300ccatttgatc gctggcagcc ataatcactc ttccccgctt cagcccgcga tacacctgat 27360gtgaaagata aaacgacacc acggtaagcg gcgttttcag gttacgccct tcacccattg 27420ccgcctctgc cgcaatggcc gttccggcaa cgacgtcaat ttctgggtgg cgttccagca 27480tctcctgcaa caggttacgc tggatttcaa tatcgttatc accaagcgca atatcaacaa 27540tacgcaccgg gcttccggca atggctgcgc gaaaaccctc gaccatctct ttactgcccc 27600cggcattatc gggtccgggc atcaacagca cgttcagtgg tttaccgtgc gcccattgca 27660ccaaatatcg cccaggttga tagcccatct gaaaccaggg tacaccaacg cggcttttca 27720cctggggagc atcaatagca tttaccagtt cgatcaccgg cagacttgct acctgctttt 27780gcagatcggg aaatgaggtc gtgctactac cgagtaaaat ggcctctgcg ccccactgtt 27840tacactggtc gatttgtgct tgctgggtag ccaactggct gtagccgcct gcctccagca 27900cttttaaatc cacaccatag cggcgagctg cctcctgcat accatagttc aacgataacc 27960agtatgaatc tttcaggctg ggataaagcg cgcacagttt ccatgcgcgt ttggctttaa 28020gcggcataga ggcttgcacc gtgaaatgct gcgcatcatg ccagcgcaac aggttatcag 28080ccgaaaatgc cggcaacatg aaaagggaaa gaagtaaaaa tagcagtacg cgcatgatag 28140cctcatcaat aataaggctt tatgctagat gcattccgct ttgcgactca acctttttca 28200ccttaagtgc accgaccgtg aatttaaccc tgacccgaag actctggatg ggctttgccc 28260tgatggcgct gttaaccctg accagtaccc tggtgggatg gtacaacctg cgctttatca 28320gccaggtgga aaaagacaac actcaggcat tgattcctac catgaatatg gcgcgccagt 28380tgagcgaagc cagcgcctgg gaacttttcg ccgcgcagaa cctgaccagt gccgataacg 28440aaaagatgtg gcaggcgcag gggcgaatgc tcaccgcaca aagcctgaag attaatgcgt 28500tgctgcaagc gttacgggaa caaggttttg ataccaccgc tattgaacaa caggagcagg 28560agatctcccg ttcattacgt cagcaagggg aactggtggg gcggcgtctg caactacgcc 28620agcaacaacg gcaactcagt cagcagatag tcgctgccgc cgatgagatc gcacgcctgg 28680cgcaaggtca ggcgaataat gcgacaactt ccgctggagc gacccaggcc gggatttacg 28740atttgatcga acaagatcag cgtcaggctg ctgaaagtgc actcgatcgg ctgattgata 28800tcgatcttga gtatgttaac cagatgaatg aactgcgcct tagcgctctg cgggtgcagc 28860aaatggtgat gaatctgggg ctggagcaga tccagaaaaa tgcaccaacg ctggaaaagc 28920agctcaataa tgcggtgaaa attctgcaac gtcggcaaat acgcattgaa gatccgggtg 28980ttcgtgcgca ggtcgcaaca acgttaacta ccgttagcca atatagcgat ttgctggcgc 29040tgtatcagca ggacagtgaa atcagcaatc acctacaaac tctcgcacaa aataacatcg 29100cccagttcgc gcagtttagt agcgaagtca gtcagctggt cgactcggta cccggggatc 29160cactcgttat tctcggacga gtgttcagta atgaacctct ggagagaacc atgtatatga 29220tcgttatctg ggttggactt ctgcttttaa gcccagataa ctggcctgaa tatgttaatg 29280agagaatcgg tattcctcat gtgtggcatg ttttcgtctt tgctcttgca ttttcgctag 29340caattaatgt gcatcgatta tcagctattg ccagcgccag atataagcga tttaagctaa 29400gaaaacgcat taagatgcaa aacgataaag tgcgatcagt aattcaaaac cttacagaag 29460agcaatctat ggttttgtgc gcagccctta atgaaggcag gaagtatgtg gttacatcaa 29520aacaattccc atacattagt gagttgattg agcttggtgt gttgaacaaa actttttccc 29580gatggaatgg aaagcatata ttattcccta ttgaggatat ttactggact gaattagttg 29640ccagctatga tccatataat attgagataa agccaaggcc aatatctaag taactagata 29700agaggaatcg attttccctt aattttctgg cgtccactgc atgttatgcc gcgttcgcca 29760ggcttgctgt accatgtgcg ctgattcttg cgctcaatac gttgcaggtt gctttcaatc 29820tgtttgtggt attcagccag cactgtaagg tctatcggat ttagtgcgct ttctactcgt 29880gatttcggtt tgcgattcag cgagagaata gggcggttaa ctggttttgc gcttacccca 29940accaacaggg gatttgctgc tttccattga gcctgtttct ctgcgcgacg ttcgcggcgg 30000cgtgtttgtg catccatctg gattctcctg tcagttagct ttggtggtgt gtggcagttg 30060tagtcctgaa cgaaaacccc ccgcgattgg cacattggca gctaatccgg aatcgcactt 30120acggccaatg cttcgtttcg tatcacacac cccaaagcct tctgctttga atgctgccct 30180tcttcagggc ttaattttta agagcgtcac cttcatggtg gtcagtgcgt cctgctgatg 30240tgctcagtat caccgccagt ggtatttatg tcaacaccgc cagagataat ttatcaccgc 30300agatggttat ctgtatgttt tttatatgaa tttatttttt gcaggggggc attgtttggt 30360aggtgagaga tctgaattgc tatgtttagt gagttgtatc tatttatttt tcaataaata 30420caattggtta tgtgttttgg gggcgatcgt gaggcaaaga aaacccggcg ctgaggccgg 30480gttattcttg ttctctggtc aaattatata gttggaaaac aaggatgcat atatgaatga 30540acgatgcaga ggcaatgccg atggcgatag tgggtatcat gtagccgctt atgctggaaa 30600gaagcaataa cccgcagaaa aacaaagctc caagctcaac aaaactaagg gcatagacaa 30660taactaccga tgtcatatac ccatactctc taatcttggc cagtcggcgc gttctgcttc 30720cgattagaaa cgtcaaggca gcaatcagga ttgcaatcat ggttcctgca tatgatgaca 30780atgtcgcccc aagaccatct ctatgagctg aaaaagaaac accaggaatg tagtggcgga 30840aaaggagata gcaaatgctt acgataacgt aaggaattat tactatgtaa acaccaggca 30900tgattctgtt ccgcataatt actcctgata attaatcctt aactttgccc acctgccttt 30960taaaacattc cagtatatca cttttcattc ttgcgtagca atatgccatc tcttcagcta 31020tctcagcatt ggtgaccttg ttcagaggcg ctgagagatg gcctttttct gatagataat 31080gttctgttaa aatatctccg gcctcatctt ttgcccgcag gctaatgtct gaaaattgag 31140gtgacgggtt aaaaataata tccttggcaa ccttttttat atccctttta aattttggct 31200taatgactat atccaatgag tcaaaaagct ccccttcaat atctgttgcc cctaagacct 31260ttaatatatc gccaaataca ggtagcttgg cttctacctt caccgttgtt cggccgatga 31320aatgcatatg cataacatcg tctttggtgg ttcccctcat cagtggctct atctgaacgc 31380gctctccact gcttaatgac attcctttcc cgattaaaaa atctgtcaga tcggatgtgg 31440tcggcccgaa aacagttctg gcaaaaccaa tggtgtcgcc ttcaacaaac aaaaaagatg 31500ggaatcccaa tgattcgtca tctgcgaggc tgttcttaat atcttcaact gaagctttag 31560agcgatttat cttctgaacc agactcttgt catttgtttt ggtaaagaga aaagtttttc 31620catcgatttt atgaatatac aaataattgg agccaacctg caggtgatga ttatcagcca 31680gcagagaatt aaggaaaaca gacaggttta ttgagcgctt atctttccct ttatttttgc 31740tgcggtaagt cgcataaaaa ccattcttca taattcaatc catttactat gttatgttct 31800gaggggagtg aaaattcccc taattcgatg aagattcttg ctcaattgtt atcagctatg 31860cgccgaccag aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata 31920actttcccca caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt 31980ggttgtaaaa acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc 32040ccaagtctgg ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt 32100aacattccgt caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca 32160acctcaagcc agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca 32220tcacctttgg taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa 32280acagggtact catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg 32340tagatttctc tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca 32400agcaatgcgg cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct 32460gactgcccca tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc 32520tttttttcat aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt 32580ttcttttttg tgctcatacg ttaaatctat caccgcaagg gataaatatc taacaccgtg 32640cgtgttgact attttacctc tggcggtgat aatggttgca tgtactaagg aggttgtatg 32700gaacaacgca taaccctgaa agattatgca atgcgctttg ggcaaaccaa gacagctaaa 32760gatctcggcg tatatcaaag cgcgatcaac aaggccattc atgcaggccg aaagattttt 32820ttaactataa acgctgatgg aagcgtttat gcggaagagg taaagccctt cccgagtaac 32880aaaaaaacaa cagcataaat aaccccgctc ttacacattc cagccctgaa aaagggcatc 32940aaattaaacc acacctatgg tgtatgcatt tatttgcata cattcaatca attgttatct 33000aaggaaatac ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac gaatcgagag 33060tgcgttgctt aacaaaatcg caatgcttgg aactgagaag acagcggaag ctgtgggcgt 33120tgataagtcg cagatcagca ggtggaagag ggactggatt ccaaagttct caatgctgct 33180tgctgttctt gaatgggggg tcgttgacga cgacatggct cgattggcgc gacaagttgc 33240tgcgattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat 33300ggagttctga ggtcattact ggatctatca acaggagtca ttatgacaaa tacagcaaaa 33360atactcaact tcggcagagg taactttgcc ggacaggagc gtaatgtggc agatctcgat 33420gatggttacg ccagactatc aaatatgctg cttgaggctt attcgggcgc agatctgacc 33480aagcgacagt ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca 33540atggacagaa tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc 33600aatgaagcca agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt 33660ggaccaaata aaaacatctc agaatggtgc atccctcaaa acgagggaaa atcccctaaa 33720acgagggata aaacatccct caaattgggg gattgctatc cctcaaaaca gggggacaca 33780aaagacacta ttacaaaaga aaaaagaaaa gattattcgt cagannnnnn tggcgaatcc 33840tctgaccagc cagaaaacga cctttctgtg gtgaaaccgg atgctgcaat tcagagcggc 33900agcaagtggg ggacagcaga agacctgacc gccgcagagt ggatgtttga catggtgaag 33960actatcgcac catcagccag aaaaccgaat tttgctgggt gggctaacga tatccgcctg 34020atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg ctgggcatgc 34080caggacaact tctggtccgg taacgtgctg agcccggcca aactccgcga taagtggacc 34140caactcgaaa tcaaccgtaa caagcaacag gcaggcgtga cagccagcaa accaaaactc 34200gacctgacaa acacagactg gatttacggg gtggatctat gaaaaacatc gccgcacaga 34260tggttaactt tgaccgtgag cagatgcgtc ggatcgccaa caacatgccg gaacagtacg 34320acgaaaagcc gcaggtacag caggtagcgc agatcatcaa cggtgtgttc agccagttac 34380tggcaacttt cccggcgagc ctggctaacc gtgaccagaa cgaagtgaac gaaatccgtc 34440gccagtgggt tctggctttt cgggaaaacg ggatcaccac gatggaacag gttaacgcag 34500gaatgcgcgt agcccgtcgg cagaatcgac catttctgcc atcacccggg cagtttgttg 34560catggtgccg ggaagaagca tccgttaccg ccggactgcc aaacgtcagc gagctggttg 34620atatggttta cgagtattgc cggaagcgag gcctgtatcc ggatgcggag tcttatccgt 34680ggaaatcaaa cgcgcactac tggctggtta ccaacctgta tcagaacatg cgggccaatg 34740cgcttactga tgcggaatta cgccgtaagg ccgcagatga gcttgtccat atgactgcga 34800gaattaaccg tggtgaggcg atccctgaac cagtaaaaca acttcctgtc atgggcggta 34860gacctctaaa tcgtgcacag gctctggcga agatcgcaga aatcaaagct aagttcggac 34920tgaaaggagc aagtgtatga cgggcaaaga ggcaattatt cattacctgg ggacgcataa

34980tagcttctgt gcgccggacg ttgccgcgct aacaggcgca acagtaacca gcataaatca 35040ggccgcggct aaaatggcac gggcaggtct tctggttatc gaaggtaagg tctggcgaac 35100ggtgtattac cggtttgcta ccagggaaga acgggaagga aagatgagcg atgaacaaac 35160tggatacgat tggattcgac aacaaaaaag acctgcttat ctcggtgggc gatttggttg 35220atcgtggtgc agagaacgtt gaatgcctgg aattaatcac attcccctgg ttcagagctg 35280tacgtggaaa ccatgagcaa atgatgattg atggcttatc agagcgtgga aacgttaatc 35340actggctgct taatggcggt ggctggttct ttaatctcga ttacgacaaa gaaattctgg 35400ctaaagctct tgcccataaa gcagatgaac ttccgttaat catcgaactg gtgagcaaag 35460ataaaaaata tgttatctgc cacgccgatt atccctttga cgaatacgag tttggaaagc 35520cagttgatca tcagcaggta atctggaacc gcgaacgaat cagcaactca caaaacggga 35580tcgtgaaaga aatcaaaggc gcggacacgt tcatctttgg tcatacgcca gcagtgaaac 35640cactcaagtt tgccaaccaa atgtatatcg ataccggcgc agtgttctgc ggaaacctaa 35700cattgattca ggtacaggga gaaggcgcat gagactcgaa agcgtagcta aatttcattc 35760gccaaaaagc ccgatgatga gcgactcacc acgggccacg gcttctgact ctctttccgg 35820tactgatgtg atggctgcta tggggatggc gcaatcacaa gccggattcg gtatggctgc 35880attctgcggt aagcacgaac tcagccagaa cgacaaacaa aaggctatca actatctgat 35940gcaatttgca cacaaggtat cggggaaata ccgtggtgtg gcaaagcttg aaggaaatac 36000taaggcaaag gtactgcaag tgctcgcaac attcgcttat gcggattatt gccgtagtgc 36060cgcgacgccg ggggcaagat gcagagattg ccatggtaca ggccgtgcgg ttgatattgc 36120caaaacagag ctgtggggga gagttgtcga gaaagagtgc ggaagatgca aaggcgtcgg 36180ctattcaagg atgccagcaa gcgcagcata tcgcgctgtg acgatgctaa tcccaaacct 36240tacccaaccc acctggtcac gcactgttaa gccgctgtat gacgctctgg tggtgcaatg 36300ccacaaagaa gagtcaatcg cagacaacat tttgaatgcg gtcacacgtt agcagcatga 36360ttgccacgga tggcaacata ttaacggcat gatattgact tattgaataa aattgggtaa 36420atttgactca acgatgggtt aattcgctcg ttgtggtagt gagatgaaaa gaggcggcgc 36480ttactaccga ttccgcctag ttggtcactt cgacgtatcg tctggaactc caaccatcgc 36540aggcagagag gtctgcaaaa tgcaatcccg aaacagttcg caggtaatag ttagagcctg 36600cataacggtt tcgggatttt ttatatctgc acaacaggta agagcattga gtcgataatc 36660gtgaagagtc ggcgagcctg gttagccagt gctctttccg ttgtgctgaa ttaagcgaat 36720accggaagca gaaccggatc accaaatgcg tacaggcgtc atcgccgccc agcaacagca 36780caacccaaac tgagccgtag ccactgtctg tcctnnnnnn attagtaata gttacgctgc 36840ggccttttac acatgacctt cgtgaaagcg ggtggcagga ggtcgcgcta acaacctcct 36900gccgttttgc ccgtgcatat cggtcacgaa caaatctgat tactaaacac agtagcctgg 36960atttgttcta tcagtaatcg accttattcc taattaaata gagcaaatcc ccttattggg 37020ggtaagacat gaagatgcca gaaaaacatg acctgttggc cgccattctc gcggcaaagg 37080aacaaggcat cggggcaatc cttgcgtttg caatggcgta ccttcgcggc agatataatg 37140gcggtgcgtt tacaaaaaca gtaatcgacg caacgatgtg cgccattatc gcctggttca 37200ttcgtgacct tctcgacttc gccggactaa gtagcaatct cgcttatata acgagcgtgt 37260ttatcggcta catcggtact gactcgattg gttcgcttat caaacgcttc gctgctaaaa 37320aagccggagt agaagatggt agaaatcaat aatcaacgta aggcgttcct cgatatgctg 37380gcgtggtcgg agggaactga taacggacgt cagaaaacca gaaatcatgg ttatgacgtc 37440attgtaggcg gagagctatt tactgattac tccgatcacc ctcgcaaact tgtcacgcta 37500aacccaaaac tcaaatcaac aggcgccgga cgctaccagc ttctttcccg ttggtgggat 37560gcctaccgca agcagcttgg cctgaaagac ttctctccga aaagtcagga cgctgtggca 37620ttgcagcaga ttaaggagcg tggcgcttta cctatgattg atcgtggtga tatccgtcag 37680gcaatcgacc gttgcagcaa tatctgggct tcactgccgg gcgctggtta tggtcagttc 37740gagcataagg ctgacagcct gattgcaaaa ttcaaagaag cgggcggaac ggtcagagag 37800attgatgtat gagcagagtc accgcgatta tctccgctct ggttatctgc atcatcgtct 37860gcctgtcatg ggctgttaat cattaccgtg ataacgccat tacctacaaa gcccagcgcg 37920acaaaaatgc cagagaactg aagctggcga acgcggcaat tactgacatg cagatgcgtc 37980agcgtgatgt tgctgcgctc gatgcaaaat acacgaagga gttagctgat gctaaagctg 38040aaaatgatgc tctgcgtgat gatgttgccg ctggtcgtcg tcggttgcac atcaaagcag 38100tctgtcagtc agtgcgtgaa gccaccaccg cctccggcgt ggataatgca gcctcccccc 38160gactggcaga caccgctgaa cgggattatt tcaccctcag agagaggctg atcactatgc 38220aaaaacaact ggaaggaacc cagaagtata ttaatgagca gtgcagatag agttgcccat 38280atcgatgggc aactcatgca attattgtga gcaatacaca cgcgcttcca gcggagtata 38340aatgcctaaa gtaataaaac cgagcaatcc atttacgaat gtttgctggg tttctgtttt 38400aacaacattt tctgcgccgc cacaaatttt ggctgcatcg acagttttct tctgcccaat 38460tccagaaacg aagaaatgat gggtgatggt ttcctttggt gctactgctg ccggtttgtt 38520ttgaacagta aacgtctgtt gagcacatcc tgtaataagc agggccagcg cagtagcgag 38580tagcattttt ttcatggtgt tattcccgat gctttttgaa gttcgcagaa tcgtatgtgt 38640agaaaattaa acaaacccta aacaatgagt tgaaatttca tattgttaat atttattaat 38700gtatgtcagg tgcgatgaat cgtcattgta ttcccggatt aactatgtcc acagccctga 38760cggggaactt ctctgcggga gtgtccggga ataattaaaa cgatgcacac agggtttagc 38820gcgtacacgt attgcattat gccaacgccc cggtgctgac acggaagaaa ccggacgtta 38880tgatttagcg tggaaagatt tgtgtagtgt tctgaatgct ctcagtaaat agtaatgaat 38940tatcaaaggt atagtaatat cttttatgtt catggatatt tgtaacccat cggaaaactc 39000ctgctttagc aagattttcc ctgtattgct gaaatgtgat ttctcttgat ttcaacctat 39060cataggacgt ttctataaga tgcgtgtttc ttgagaattt aacatttaca acctttttaa 39120gtccttttat taacacggtg ttatcgtttt ctaacacgat gtgaatatta tctgtggcta 39180gatagtaaat ataatgtgag acgttgtgac gttttagttc agaataaaac aattcacagt 39240ctaaatcttt tcgcacttga tcgaatattt ctttaaaaat ggcaacctga gccattggta 39300aaaccttcca tgtgatacga gggcgcgtag tttgcattat cgtttttatc gtttcaatct 39360ggtctgacct ccttgtgttt tgttgatgat ttatgtcaaa tattaggaat gttttcactt 39420aatagtattg gttgcgtaac aaagtgcggt cctgctggca ttctggaggg aaatacaacc 39480gacagatgta tgtaaggcca acgtgctcaa atcttcatac agaaagattt gaagtaatat 39540tttaaccgct agatgaagag caagcgcatg gagcgacaaa atgaataaag aacaatctgc 39600tgatgatccc tccgtggatc tgattcgtgt aaaaaatatg cttaatagca ccatttctat 39660gagttaccct gatgttgtaa ttgcatgtat agaacataag gtgtctctgg aagcattcag 39720agcaattgag gcagcgttgg tgaagcacga taataatatg aaggattatt ccctggtggt 39780tgactgatca ccataactgc taatcattca aactatttag tctgtgacag agccaacacg 39840cagtctgtca ctgtcaggaa agtggtaaaa ctgcaactca attactgcaa tgccctcgta 39900attaagtgaa tttacaatat cgtcctgttc ggagggaaga acgcgggatg ttcattcttc 39960atcactttta attgatgtat atgctctctt ttctgacgtt agtctccgac ggcaggcttc 40020aatgacccag gctgagaaat tcccggaccc tttttgctca agagcgatgt taatttgttc 40080aatcatttgg ttaggaaagc ggatgttgcg ggttgttgtt ctgcgggttc tgttcttcgt 40140tgacatgagg ttgccccgta ttcagtgtcg ctgatttgta ttgtctgaag ttgtttttac 40200gttaagttga tgcagatcaa ttaatacgat acctgcgtca taattgatta tttgacgtgg 40260tttgatggcc tccacgcacg ttgtgatatg tagatgataa tcattatcac tttacgggtc 40320ctttccggtg atccgacagg ttacg 40345345793DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 34tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtaggga 360taacagggta atgaattgaa attacgcccc gccctgccac tcatcgcagt actgttgtaa 420ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc 480cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc 540gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt 600ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc 660gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc 720actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac 780actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt 840catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac 900ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac 960tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata 1020tccagtgatt tttttctcca ttttagcttc cttagctcca gatctatgga tcgctcgaaa 1080aaatcaaatc atcaatttct gagcttgctg taatttcttg tttatcaaca tatgaagtca 1140tacctgtttt actcctcaag ataatattag aaagtatggc agcactgctg tcatactctt 1200ttataccctt catctttcaa gctgctgctt tgttggctgc tttcactcac cccagtcaca 1260tagttatcta tgctcctggg gattcgttca cttgccgcct cgctgcaact cgaaatctat 1320taggtatatt ccatgtggta cttcttaata ttatcatcaa caatattgat tacatttttt 1380tggctcatca aatcattcat tggttcaaag gacagcaata cacttttcgc accacacttt 1440tcaattgcca acttagccgc agttatacac tccgtataat ttccgacagc gttttctgca 1500attatttctt caagtttatt ttcgaaattt tcattagggt gcatttcaag aacataatca 1560ctaataaatg cacgcgtctc ttgtttagct ttattactat cttcgttata gttaactaat 1620atcattaact gatggtctat ctctgatagg tcaacgtcat atttatccgc aacggcttta 1680tatctttcag catattcata tctaacatca ttagaatcat cccacttaaa gatgagagga 1740ataccttttt tggccgccca ctcaacaata tgatgactgg ttgctgttac atatttccga 1800ggtccgcctg gcgtataagc atggggattt acagatattt tagggaagct ataaaaatcg 1860ttatctggat tacaatagcc tgttgttaaa gcatcgttaa tgatttcata acactcttca 1920aatagttgct gttgatattc aaccgggcga ttaaaaaaat gcatttcatc ttttttttcg 1980caatcactaa accctaaaat aaatctccct tcacttaact gatccaataa gcaagcttcc 2040tccgctatgg cgacaggatg atgagttgta atgatgtgat ttaatgaacc aattttaatt 2100ttctctgtta aaccgagcag aaaaccagaa acagtcagag gagcgccgac aacaccatta 2160tctgaaaaat gattttcata cactaaaatc tgttcaaaat tcaacttatc aacatactcc 2220gttatttcct gcatgcgaac tatactttgt tcttgaacag ttgttgaatt gatgaagtta 2280aggaagaaca atccaaattt catttctttc tccttagcta atataatagc gaacgttgtt 2340tttctttaag aaatggcatg acatcagact ggaagagctt catggaagca ataatttcgt 2400ctactgttcc attagcttca aatccacaac aaatatttga tattcctgta gcatcaatgt 2460ctttttgaat tatgtcaata cattcctgcg gcgttcccac gggattgatt tcgtaactgt 2520aatcaatacg gcgattagta tctttatgtc cttttaatac aaagtcacgc cactgccctt 2580tattgaaatc ataacctctt gtttggtctg aatcatcaaa aatagtcgta gcattcacat 2640aagaatcata ccaatgcccc agaaatttcc ggcaaatctc tttcgcttta attgagtcat 2700gatctacaga tgttatatat gataagcaat ggtcgatatt atgaatatcg tgcccatatt 2760cttgagccac ttcattataa agctcaagtt gtgctttctt ttcgttagta tttataatcc 2820aacttaatat catcggtagg ccaaattgag cagcccactc agtcgtcgaa gctgattcag 2880ccaccacata aaccggtgcg ccacctctgc tatacgccgc ggggtttact tttaccttat 2940ggaacttgat atgttcatta tcagcttcca tatatccctc tgtcatgcca ttctttatca 3000gcccgtacca gcattccgct aaggcgcgac tgttattcat atctgtgccg aatacgcgaa 3060agtccttgtt gtaaagccct cggcaaatac caaaccgaaa tcgtcctttt gacatttgat 3120ccaataaatt cacatcttca agttggcgta ctggatgggc tgtgggaaga acaatagcgg 3180cagttcctac attcaatttt ttagtcgcgc caagtaaata tgcagcagcg acataagggt 3240taccaagcaa accaaactcc gtgaaatgat gctccagtaa ccatacggta tcaaaaccac 3300actcctcaga gatgcgacct aatttaacca aacgtttcat tacctctgtt tgagaaaatt 3360ggggaggttg gtatgtaagc aaaaagtttc caaatttcat agagagtcct tatattgcta 3420tttgagtgat agaatatctc aatagatttt aagacagaga aattgcttga ttttcaatct 3480caattctcat tcggcgttca ttgactgtcg caatagttaa atgttcaaat gacggttcag 3540taatatcaac atcaatatcc agatgatcat tatccatcgt actgcgcgaa ttataacata 3600attttttata aaattcaaat ttatgcaggc atgcaagtcg acattaccct gttatcccta 3660ctgcaggcat gcaagcttgc caacgactac gcactagcca acaagagctt cagggttgag 3720atgtgtataa gagacagctg tcttaatgaa tcggccaacg cgcggggaga ggcggtttgc 3780gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3840ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 3900acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 3960cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 4020caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 4080gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 4140tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 4200aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 4260ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 4320cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 4380tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 4440tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 4500ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 4560aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4620aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 4680aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 4740gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 4800gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 4860caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 4920ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 4980attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 5040ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 5100gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 5160ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 5220tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 5280gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 5340cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 5400gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 5460tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 5520ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5580gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5640tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 5700catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 5760ataaaaatag gcgtatcacg aggccctttc gtc 5793

Claims

1. A method for detecting a target molecule using a protein VII (pVII)-modified bacteriophage, comprising: a) contacting an immobilized target molecule with a bacteriophage encoding a luciferase and comprising a target-molecule specific insert in pVII, wherein the bacteriophage specifically binds the target molecule, under conditions that promote binding of the bacteriophage to the target molecule, to produce a target molecule-bacteriophage complex; b) contacting the intact target molecule-bacteriophage complex with a bacterial strain susceptible to infection by the bacteriophage; c) incubating the target molecule-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the luciferase, to produce a bacteriophage-infected bacterial culture comprising luciferase; d) adding to the bacteriophage-infected bacterial culture a substrate for the luciferase; and e) measuring the presence and/or magnitude of fluorescence produced from the action of the luciferase on its substrate, thereby detecting the presence of the target molecule.

2. The method of claim 1, wherein the target molecule comprises a first affinity tag and the bacteriophage comprises a second affinity tag, wherein the first and second affinity tags specifically bind.

3. The method of claim 2, wherein the first and second affinity tags are enzymatically or chemically coupled to the target molecule and the bacteriophage.

4. The method of claim 2, wherein the first affinity tag is streptavidin and the second affinity tag is biotin.

5. The method of claim 1, wherein the bacteriophage is engineered to bind directly to the target molecule using phage display.

6. The method of claim 1, wherein the bacteriophage is derived from an M13, T, T7, or .lamda. bacteriophage.

7. The method of claim 1, wherein the bacteriophage comprises SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, or SEQ ID NO:4.

8. A method for detecting a target molecule using a protein VII (pVII)-modified bacteriophage, comprising: a) contacting an immobilized target molecule with a first affinity ligand that specifically binds the target molecule, under conditions that promote target molecule-affinity ligand binding, to form a target molecule-first affinity ligand complex, and optionally contacting the target molecule-first affinity ligand complex with one or more additional affinity ligands under conditions that promote affinity ligand-affinity ligand binding, to produce a target molecule-first affinity ligand-additional affinity ligand complex, wherein the one or more affinity ligands are added sequentially, and wherein each successive affinity ligand specifically binds to the affinity ligand added immediately previous; b) contacting the target molecule-first affinity ligand complex or the target molecule-first affinity ligand-additional affinity ligand complex with a bacteriophage encoding a luciferase and comprising an affinity ligand-specific insert at pVII, wherein the bacteriophage binds to the first affinity ligand, or if one or more additional affinity ligands are used, binds to the ultimate affinity ligand, to form a target molecule-affinity ligand-bacteriophage complex; c) contacting the intact target molecule-affinity ligand-bacteriophage complex with a bacterial strain susceptible to infection by the bacteriophage; d) incubating the target molecule-affinity ligand-bacteriophage complex with the bacterial strain under conditions that promote the propagation of the bacteriophage in the bacteria and expression of the luciferase, to produce a bacteriophage-infected bacterial culture comprising luciferase; e) adding to the bacteriophage-infected bacterial culture a substrate for the luciferase; and f) measuring the presence and/or magnitude of fluorescence produced from the action the luciferase on its substrate, thereby detecting the presence of the target molecule.

9. The method of claim 8, wherein the first affinity ligand, or if one or more additional affinity ligands are used, the ultimate affinity ligand, comprises a first affinity tag, and the bacteriophage comprises a second affinity tag, wherein the first and second affinity tags specifically bind.

10. The method of claim 8, wherein the first and second affinity tags are enzymatically or chemically coupled to the affinity ligand and the bacteriophage.

11. The method of claim 8, wherein the first affinity tag is streptavidin and the second affinity tag is biotin.

12. The method of claim 8, wherein the bacteriophage is engineered to bind directly to the affinity ligand using phage display.

13. The method of claim 8, wherein the bacteriophage is derived from an M13, T, 7, or .lamda. bacteriophage.

14. The method of claim 8, wherein the bacteriophage comprises SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, or SEQ ID NO:4.

15. A protein VII (pVII)-modified bacteriophage comprising a nucleic acid encoding a luciferase, wherein the bacteriophage comprises a target-specific insert at protein VII (pVII).

16. The bacteriophage, wherein the bacteriophage is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and SEQ ID NO: 4.

17. The bacteriophage of claim 15, wherein the bacteriophage comprises an affinity tag.

18. The bacteriophage nucleic acid of claim 17, wherein the affinity tag is enzymatically or chemically coupled to the bacteriophage.

19. The bacteriophage nucleic acid of claim 15, wherein the bacteriophage is engineered to bind directly to an affinity ligand or to a target molecule using phage display.

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