WOODY PLANTS HAVING IMPROVED GROWTH CHARACTERISTICS AND METHOD FOR MAKING THE SAME USING TRANSCRIPTION FACTORS

Abstract

An extensive analytical platform for selecting genes with a possible commercial phenotype from a large group of candidate genes identified using tools in bioinformatics, data from EST sequencing and DNA array is disclosed. Methods are disclosed for producing a transgenic plant having an increased growth compared to its wild type by altering in the plant the level of a gene product of at least one gene specifically expressed during different phases of wood formation. This may be done with transgenic methods or by specific crossing methods. Plant cells or plant progeny and wood having modulated gene expression are also disclosed. A DNA construct with a nucleotide sequence for a gene producing a transgenic plant having an increased growth compared to its wild type and a plant cell or plant progeny having the DNA construct are also disclosed.

Description

TECHNICAL FIELD OF THE INVENTION

[0001] The present invention relates generally to the field of molecular biology and relates to a method for improving plant growth characteristics. More specifically, the invention relates to methods for phenotypically modifying plants and transgenic plants and plants obtained by a specific crossing method having altered expression of a gene resulting in a modified growth phenotype. The invention also provides constructs useful in the method of the invention. Further, the invention relates to a plant cell or plant progeny of the plants and wood produced by the plants according to the invention.

BACKGROUND OF THE INVENTION

[0002] At present, the primary objectives of forest-tree engineering and molecular breeding are to improve wood quality and yield. The global demand for wood products is growing at around 1.7% annually, and this increase in wood consumption is occurring despite the fact that the maximum sustainable rate of harvesting from the worlds forests has already been reached or exceeded. Therefore, there is a need for increases in plantation wood production worldwide. Forestry plantations may also have advantages as a carbon sequestration crop in response to increasing atmospheric CO₂. Similarly, increased production of biomass from non-woody plants is desirable, for instance in order to meet the demand for raw material for energy production. Modification of specific processes during cell development in higher species is therefore of great commercial interest, not only when it comes to improving the properties of trees, but also other plants.

[0003] Plant growth by means of apical meristems results in the development of sets of primary tissues and in lengthening of the stem and roots. In addition to this primary growth, tree species undergo secondary growth and produce the secondary tissue "wood" from the cambium. The secondary growth increases the girth of stems and roots.

[0004] Perennial plants such as long-lived trees have a life style considerably different from annual plants such as Arabidopsis in that perennial plants such as trees has an indeterminate growth whereas plants like Arabidopsis have an terminate end of growth when the plant flowers. The final size of an Arabidopsis plant is in many ways dependent on the developmental program from germination to flowering and seed set. One example is that any change in the timing of these events can drastically change the size of the plant.

[0005] Perennial plants also cycle between periods of active growth and dormancy. During active growth leaves perform photosynthesis to capture energy which then used to drive various cellular processes. The fixed carbon which converted to sucrose is transferred to stem tissues and apical bud where it is stored during the dormant state initially as starch and later as sucrose. As growth reinitiates after release from dormancy, this sucrose is translocated to actively growing tissues since early stages of reactivation occur before photosynthesis starts. Similarly for nitrogen, amino acids are translocated also to stem and apical tissues and stored as storage proteins during dormancy and broken down as growth starts. Thus the life cycle of long lived trees differs significantly from annual crops which often translocate carbon and nitrogen to seeds. Due to these differences between annual crops and perennial plants such as trees, determinants of yield and the ability to measure them are likely to considerably different. Actually, in many instances is a model system such as Populus tremula×tremuloides much better for reliably finding genes that can be used for increasing biomass production. For example for annual crops, seed size/yield has been proposed to be a measure of plant size and productivity but this is unlikely to be the case since perennial plants such as trees take several years to flower and thus seed yield, if at all, is only indicator of growth conditions that prevail during the year the plant flowered. Thus direct translation of research and findings from annual crops are unlikely to be useful in case of trees.

[0006] A very important part of the biomass of trees is present in stem tissues. This biomass accumulation is a result of leaf photosynthesis and nitrogen acquisition and redistribution of nutrients to various cellular processes. As such leaf size, leaf photosynthesis, ability to acquire nitrogen size of root system can all be important players in determination of plant productivity and biomass production. However none by themselves can account for the entire biomass production. For example, leaf size is not always related to biomass as significant variation can be found in leaf size. Moreover the ability to cope with stress is an important determinant of biomass production. Thus there are several factors that need to be altered in order to enhance biomass production in trees.

[0007] Furthermore, wood density is an important trait in increased biomass production, an increased wood density gives less volume that have to be transported and contain more energy content per volume. Therefore increased density is of interest even if the total biomass is not increased. Density is also a important in showing that an increased metrical growth in height and diameter is not coupled to an decrease in wood density.

[0008] One way to increase growth is to learn more about gene function and use that information to increase growth and biomass production. Such gene function knowledge and ways to use the knowledge is described in this patent.

[0009] Most genes have now been identified in a number of plants such as Arabidopsis thaliana (Arabidopsis Genome Initiative 2000) and Populus tremula×tremuloides (Sterky et al. 2004) and Populus trichocarpa (Tuskan et al. 2006).

[0010] Hertzberg et al. 2001, and Schrader et al. 2005 have used transcript profiling to reveal a transcriptional hierarchy for thousands of genes during xylem development as well as providing expression data. Such data can facilitate further elucidation of many genes with unknown function White et al. 1999; Aharoni et al. 2000.

[0011] One problem remaining is how to identify the potentially most important genes involved in regulation of cell division and other processes related to growth. In this present invention we examined a number of transcription factors for their use, which resulted in an unexpectedly increased growth when over expressed. The reason to select transcription factors for analysis is because they are known to be part regulators of many if not most processes in living organisms including plants. It is predictive that Arabidopsis thaliana contains 1500 different transcription factors that can be divided into ˜30 subclasses based on sequence homologies (Riechmann et al. 2000). The function a certain transcription factor have within a plant is closely connected to which genes it regulates, e.g. although transcription factors within a transcription factor sub group as the MYB class are similar, they are known to regulate several different processes in plants. Transcription factors are proteins that regulate transcription of genes by either repressing or activating transcription initiation of specific genes or genomic regions containing different genes.

[0012] Specifically targeting transcription factors in plants in order to find genes that can be used to alter plant characteristics have been done before. In for example WO 02/15675, a large numbers of transcription factors have been analysed and the possible use for many of them been mentioned. US2007/0039070 describes and lists a large number of transcription factor genes from Eucalyptus and Pinus radiata and speculates in the use of such genes. Here we present specific transcription factors that have an industrially relevant effect in substantially increasing growth, which is supported with experimental data.

[0013] Although it is obvious that results from EST programs, genome sequencing and expression studies using DNA array technologies can verify where and when a gene is expressed it is rarely possible to clarify the biological and/or technical function of a gene only from these types of analytical tools. In order to analyze and verify the gene function a functional characterization must be performed, e.g. by gene inactivation and/or gene over-expression. However, in order to be able to identify genes with interesting and most often unexpected commercial features, candidate genes has to be evaluated based on functional analysis and measuring increased growth with multiple criteria.

[0014] MYB transcription factors. One of the genes presented here (SEQ ID:12) belongs to the MYB class of transcription factors. The MYB transcription factor family is predicted to have ˜180 members in Arabidopsis (Riechmann et al 2000). Several different functions have been found for MYB genes in plants (Jin and Martin 1999). More specifically genes closely related to SEQ ID: 12 have not to our knowledge been shown to be involved in regulating growth rates and biomass production. The closely related genes AT2G01060 and AB192880 are implicated to be involved in biotic stress responses, US2003101481 and Katou et al 2005.

[0015] SET domain transcription factors (Ng et al. 2007). One of the genes presented here SEQ ID: 11 belong to the SET domain class of transcription factors. SET domain proteins regulate transcription by modulating chromatin structure. The Arabidopsis genome is known to contain at least 29 active set domain proteins. Genes closely related to SEQ ID: 11 have not to our knowledge been shown to be involved in regulating growth rates and biomass production.

[0016] The bHLH class of transcriptional regulators is an large group of transcription factors in plants, for example is Arabidopsis thaliana predicted to contain ˜139 members (Riechmann et al 2000). bHLH proteins have been implicated in many different processes se Xiaoxing et al 2006 for an overview in rice. One of the genes presented here SEQ ID: 10 belong to the bHLH class of transcription factors. Genes closely related to SEQ ID:10 have not to our knowledge been shown to be involved in regulating growth rates and biomass production.

[0017] The gene SEQ ID: 9 belong to the Homeobox class of genes. The closest Arabidopsis thaliana homolog to the gene over-expressed with construct TF0013 is predicted to be AT1G23380. Over-expression of a related Solanum tuberosum homolog to the gene over-expressed with construct TF0013 decreases growth, internode length and leaf size (U.S. Pat. No. 7,265,263). Over-expression of a related Arabidopsis thaliana homolog to the gene over-expressed with construct TF0013 alters leaf morphology (U.S. Pat. No. 7,265,263, US 20070022495, and WO01036444). The use to increase yield and biomass production by altering the expression level of the gene over-expressed with construct TF0013 is previously unknown.

[0018] The IAA/AUX group of transcription factors is a small group of transcription factors mainly found in plants (26 members predicted in Arabidopsis by Riechmann et al. 2000). The gene corresponding to SEQ ID: 13 belong to this group and is described in Moyle et al 2002. Genes closely related to SEQ ID: 13 have not to our knowledge been shown to be involved in regulating growth rates and biomass production.

[0019] The WRKY gene family group. The WRKY transcription factor family is a large family of genes in plants. Rice is predicted to have more than 90 members and Arabidopsis is predicted to have 74 members (Ulker and Somssich 2004). One of the functions that have been mostly associated with WRKY genes are wound and pathogen defense signalling, but also signalling coupled to abiotic stress, and resistance against both abiotic and biotic stress.

[0020] Eight of the genes presented here belong to the WRKY class of transcription factors.

[0021] SEQ ID:4 and SEQ ID:7 belongs to one sub group of WRKY genes. Genes closely related to SEQ ID:4 and SEQ ID:7 have not, to our knowledge, been shown to be involved in regulating growth rates and biomass production.

[0022] SEQ ID:1 belongs to another sub group of WRKY genes. A closely related Arabidopsis thaliana homolog (AT2G23320) to the gene SEQ ID:1 is believed to be involved in C/N sensing (US 20060272060), altering leaf size (U.S. Pat. No. 7,238,860, US 20030226173, US 20040019927, and WO02015675) and altering seed protein content (US 20030226173). AT2G23320 is also believed to be involved in the reaction and adaptation to peroxide stress according to Patent Application No. WO04087952. US 20040019927, U.S. Pat. No. 7,238,860, US 20030226173, WO02015675 mention the gene AT2G23320 in combination with increased leaf size and increased stature and speculate that over expression of this gene can be used to increase growth and biomass production. We have here shown that SEQ ID:1 can be used in trees to increase growth to an industrial significant degree.

[0023] SEQ ID:6 belongs to an sub group of WRKY genes that is related to the subgroup that SEQ ID:1 belongs to but clearly different from that group of genes. Genes closely related to this gene are known to be negative regulators of basal resistance in Arabidopsis thaliana. Journot-Catalino eta al 2006. The closely related gene AT4G31550 is believed to be related to seed prenyl lipid and seed lutein levels (US 20060195944 and US 20070022495, and WO01035727). Another predicted Arabidopsis thaliana homolog AT2G24570 to SEQ ID:6 is believed to be involved in C/N sensing (US 20070022495 and 20060272060). Genes closely related to SEQ ID:6 have not to our knowledge been shown to be involved in regulating growth rates and biomass production.

[0024] SEQ ID:2 belongs to another sub group of WRKY genes. Genes closely related to SEQ ID:2 have not to our knowledge been shown to be involved in regulating growth rates and biomass production.

[0025] SEQ ID:3 and SEQ ID:5 belongs to a large group of WRKY genes containing 2 WRKY domains. A number of related homologs to SEQ ID:3 and SEQ ID:5 containing two WRKY-domains are believed to be involved in altering seed yield and number of flowers in Oryza sativa according to Patent Application No. WO 2007003409. The use to increase growth and biomass production by altering the expression level is previously unknown.

[0026] SEQ ID:8 belongs to another sub group of WRKY genes. The closely related Arabidopsis thaliana gene AT4G23810 is known to reduce plant size and be involved in altering seed protein content (US 20030226173). Another related Arabidopsis thaliana homolog (AT5G24110) is known to be involved in altering seed protein content and inducing early flowering (US 20030226173). Genes closely related to SEQ ID:8 have not to our knowledge been shown to be involved in regulating growth rates and biomass production.

SUMMARY OF THE INVENTION

[0027] The present invention pertains to novel genes that can be used to increase growth. The genes are found by using a analytical platform that is concentrated on analysing growth behavior based on a combination of multiple criteria. The invention provides methods for producing a transgenic plant by changing the expression of one or more genes selected from a group of genes which fulfil said criteria. Thus, the invention relates to methods for phenotypically modifying plants and transgenic plants and plants obtained by a specific crossing method having altered expression of a gene resulting in a modified growth phenotype. The invention also provides constructs useful in the method of the invention. Further, the invention relates to a plant cell or plant progeny of the plants and wood produced with unexpectedly good properties by the plants according to the invention.

[0028] A number of genes analyzed using the analytical platform show interesting and most often unexpected commercial features. Thus, an aspect of the present invention provides a method of producing a plant having an unexpectedly high growth compared to its wild type, comprising altering (increasing) in the plant the level of a gene product of at least one gene belonging to one of the transcription factor sequences SEQ ID:1-13, 97-115.

[0029] The increase in growth can be observed when comparing a group of transgenic plants grown for 8 weeks in a greenhouse under a photoperiod of 18 hours, a temperature of 22° C./15° C. (day/night) and a weekly fertilization Weibulls Rika S NPK 7-1-5 diluted 1 to 100 with a group of wild-type plants grown under identical conditions;

[0030] Another aspect of the invention provides a plant cell or plant progeny of a transgenic plant or a plant with intentionally changed (increased) levels of one gene's SEQ ID: 1-13, 97-115 according to the invention and comprising a recombinant polynucleotide.

[0031] A further aspect of the invention provides biomass and products thereof produced by a intentionally plant having the characteristics described above.

[0032] Still another aspect of the invention provides a DNA construct comprising at least one sequence as described as described herein.

[0033] Finally, one aspect of the invention provides a plant cell or plant progeny comprising the DNA construct according to the invention.

DESCRIPTION OF THE FIGURES

[0034] FIG. 1 shows an example of a height growth curve with four different data point linear regression lines shown, the black regression line shows the maximum height growth rate;

DETAILED DESCRIPTION OF THE INVENTION

Definitions

[0035] Prior to discussing the present invention in further details, the following terms and conventions will first be defined:

[0036] The term "transgenic plant" refers to a plant that contains genetic material, not found in a wild type plant of the same species, variety or cultivar. The genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty. Typically, the foreign genetic material has been introduced into the plant by human manipulation. The term also refers to plants in which genetic material has been inserted to function as a selection marker. Examples of such selectable markers include kanamycin, hygromycin, phosphoinotricin, chlorsulfron, methotrexate, gentamycin, spectinomycin, imidazolinones, d-aminoacids and glyphosate.

[0037] In the present context the term "growth" includes primary growth, including a lengthening of the stem and roots, as well as secondary growth of a plant, including production of secondary tissue, "wood", from the cambium and an increase in the girth of stems and roots. Thus, the expression "increased growth" relates in the present context to an increase growth of a transgenic plant relative to the wild-type plant from which the transgenic plant is derived, when grown under the same growth conditions. As described below, a transgenic plant is characterized to have an increased growth if the plant meets at least one of the "growth difference selection criteria" as defined in the below Examples.

[0038] The term "phenotype" refers in the present context to an individual plant's total physical appearance, such as growth. Examples of different growth phenotypes used in the present context are listed in the below table 1.2.

[0039] The term "gene" broadly refers to any segment of DNA associated with a biological function. Genes include coding sequences and/or regulatory sequences required for their expression. Genes also include non-expressed DNA nucleic acid segments that, e.g., form recognition sequences for other proteins (e.g., promoter, enhancer, or other regulatory regions). Genes can be obtained from a variety of sources, including cloning from a source of interest or synthesizing from known or predicted sequence information, and may include sequences designed to have desired parameters.

[0040] "Overexpression" refers to the expression of a polypeptide or protein encoded by a DNA of SEQ ID NO: 1-13, 97-115 or similar sequences introduced into a host cell, wherein said polypeptide or protein is either not normally present in the host cell, or wherein said polypeptide or protein is present in said host cell at a higher level than that normally expressed from the endogenous gene encoding said polypeptide or protein.

[0041] Overexpression of the proteins of the instant invention may be accomplished by first constructing a chimeric gene in which the coding region is operably linked to a promoter capable of directing expression of a gene in the desired tissues at the desired stage of development. The chimeric gene may comprise promoter sequences and translation leader sequences derived from the same genes. 3' Non-coding sequences encoding transcription termination signals may also be provided. The instant chimeric gene may also comprise one or more introns in order to facilitate gene expression. A suitable promoter may be the CaMV 35 S promoter which may be used with Agrobacterium as a vector.

[0042] The term "RNA interference" or "RNAi" refers generally to a process in which a double-stranded RNA molecule or a short hairpin RNA changes the expression of a nucleic acid sequence with which they share substantial or total homology.

[0043] The term "RNAi down-regulation" refers to the reduction in the expression of a nucleic acid sequence mediated by one or more RNAi species. The term "RNAi species" refers to a distinct RNA sequence that elicits RNAi.

[0044] The term "photoperiod" refers to the daily cycle of light and darkness.

[0045] The terms "nucleic acid construct", "DNA construct" and "vector" refer to a genetic sequence used to transform plants or other organisms. The nucleic acid construct or DNA construct may be able to direct, in a transformed plant the expression of a protein or a nucleic acid sequence, such as for example an antisense RNA. Typically, such a nucleic acid construct or DNA construct comprises at least a coding region for a desired gene product or a desired nucleic acid product operably linked to 5' and 3' transcriptional regulatory elements. In some embodiments, such nucleic acid constructs or DNA constructs are chimeric, i.e. consisting of a mixture of sequences from different sources. However, non-chimeric nucleic acid constructs or DNA constructs may also be used in the present invention.

[0046] The term "recombinant" when used with reference, e.g., to a cell, nucleotide, vector, protein, or polypeptide typically indicates that the cell, nucleotide, or vector has been modified by the introduction of a heterologous (or foreign) nucleic acid or the alteration of a native nucleic acid, or that the protein or polypeptide has been modified by the introduction of a heterologous amino acid, or that the cell is derived from a cell so modified. Recombinant cells express nucleic acid sequences (e.g., genes) that are not found in the native (non-recombinant) form of the cell or express native nucleic acid sequences (e.g. genes) that would be abnormally expressed under-expressed, or not expressed at all. The term "recombinant" when used with reference to a cell indicates that the cell replicates a heterologous nucleic acid, or expresses a peptide or protein encoded by a heterologous nucleic acid. Recombinant cells can contain genes that are not found within the native (non-recombinant) form of the cell. Recombinant cells can also contain genes found in the native form of the cell wherein the genes are modified and re-introduced into the cell by artificial means. The term also encompasses cells that contain a nucleic acid endogenous to the cell that has been modified without removing the nucleic acid from the cell; such modifications include those obtained by gene replacement, site-specific mutation, and related techniques.

[0047] The term "nucleic acid sequence" refers to a polymer of deoxyribonucleotides or ribonucleotides in either single- or double-stranded form. Unless specifically limited, the term encompasses nucleic acid sequences containing known analogues of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences and as well as the sequence explicitly indicated.

[0048] A "polynucleotide" is a nucleic acid sequence comprising a plurality of polymerized nucleotide residues, e.g., at least about 15 consecutive polymerized nucleotide residues, optionally at least about 30 consecutive nucleotides, at least about 50 consecutive nucleotides. In many instances, a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof. Additionally, the polynucleotide may comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5' or 3' untranslated regions, a reporter gene, a selectable marker, or the like. The polynucleotide can be single stranded or double stranded DNA or RNA. The polynucleotide optionally comprises modified bases or a modified backbone. The polynucleotide can be e.g. genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. The polynucleotide can comprise a sequence in either sense or antisense orientation.

[0049] The term "polypeptide" is used broadly to define linear chains of amino acid residues, including occurring in nature and synthetic analogues thereof.

[0050] In the context of the present invention "complementary" refers to the capacity for precise pairing between two nucleotides sequences with one another. For example, if a nucleotide at a certain position of an oligonucleotide is capable of hydrogen bonding with a nucleotide at the corresponding position of a DNA or RNA molecule, then the oligonucleotide and the DNA or RNA are considered to be complementary to each other at that position. The DNA or RNA strand are considered complementary to each other when a sufficient number of nucleotides in the oligonucleotide can form hydrogen bonds with corresponding nucleotides in the target DNA or RNA to enable the formation of a stable complex.

[0051] In the present context the expressions "complementary sequence" or "complement" therefore also refer to nucleotide sequences which will anneal to a nucleic acid molecule of the invention under stringent conditions.

[0052] The term "stringent conditions" refers to general conditions of high, weak or low stringency.

[0053] The term "stringency" is well known in the art and is used in reference to the conditions (temperature, ionic strength and the presence of other compounds such as organic solvents) under which nucleic acid hybridisations are conducted. With "high stringency" conditions, nucleic acid base pairing will occur only between nucleic acid fragments that have a high frequency of complementary base sequences, as compared to conditions of "weak" or "low" stringency. Suitable conditions for testing hybridization involve pre-soaking in 5×SSC and pre-hybridizing for 1 hour at ˜40° C. in a solution of 20% formamide, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 mg of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 100 mM ATP for 18 hours at ˜40° C., followed by three times washing of the filter in 2×SSC, 0.2% SDS at 40° C. for 30 minutes (low stringency), preferred at 50° C. (medium stringency), more preferably at 65° C. (high stringency), even more preferably at ˜75° C. (very high stringency). More details about the hybridization method can be found in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor, 1989.

[0054] The terms "hybridization" and "hybridize" are used broadly to designate the association between complementary or partly complementary nucleic acid sequences, such as in a reversal of the process of denaturation by which they were separated. Hybridization occurs by hydrogen bonding, which may be Watson-Crick, Hoogsteen, reversed Hoogsteen hydrogen bonding, etc., between complementary nucleoside or nucleotide bases. The four nucleobases commonly found in DNA are G, A, T and C of which G pairs with C, and A pairs with T. In RNA T is replaced with uracil (U), which then pairs with A. The chemical groups in the nucleobases that participate in standard duplex formation constitute the Watson-Crick face. Hoogsteen showed a couple of years later that the purine nucleobases (G and A) in addition to their Watson-Crick face have a Hoogsteen face that can be recognised from the outside of a duplex, and used to bind pyrimidine oligonucleotides via hydrogen bonding, thereby forming a triple helix structure.

[0055] A "subsequence" or a "fragment" is any portion of an entire sequence. Thus, a fragment or subsequence refers to a sequence of amino acids or nucleic acids that comprises a part of a longer sequence of amino acids (e.g. polypeptide) or nucleic acids (e.g. polynucleotide), respectively.

[0056] In the present context, the term "homology" indicates similarities between two amino acid sequences or between two nucleotide sequences is described by the parameter "sequence identity".

[0057] The term "sequence identity" indicates a quantitative measure of the degree of homology between two amino acid sequences or between two nucleic acid sequences of equal length. If the two sequences to be compared are not of equal length, they must be aligned to give the best possible fit, allowing the insertion of gaps or, alternatively, truncation at the ends of the polypeptide sequences or nucleotide sequences. The sequence identity can be calculated as

( N ref - N dif ) 100 N ref , ##EQU00001##

wherein N_dif is the total number of non-identical residues in the two sequences when aligned and wherein N_ref is the number of residues in one of the sequences. Hence, the DNA sequence AGTCAGTC will have a sequence identity of 75% with the sequence AATCAATC (N_dif=2 and N_ref=8). A gap is counted as non-identity of the specific residue(s), i.e. the DNA sequence AGTGTC will have a sequence identity of 75% with the DNA sequence AGTCAGTC (N_dif=2 and N_ref=8).

[0058] With respect to all embodiments of the invention relating to nucleotide sequences, the percentage of sequence identity between one or more sequences may also be based on alignments using the clustalW software (http:/www.ebi.ac.uk/clustalW/index.html) with default settings. For nucleotide sequence alignments these settings are: Alignment=3Dfull, Gap Open 10.00, Gap Ext. 0.20, Gap separation Dist. 4, DNA weight matrix: identity (IUB). Alternatively, the sequences may be analysed using the program DNASIS Max and the comparison of the sequences may be done at http://www.paraliqn.org/. This service is based on the two comparison algorithms called Smith-Waterman (SW) and ParAlign. The first algorithm was published by Smith and Waterman (1981) and is a well established method that finds the optimal local alignment of two sequences The other algorithm, ParAlign, is a heuristic method for sequence alignment; details on the method is published in Rognes (2001). Default settings for score matrix and Gap penalties as well as E-values were used.

[0059] The phrase "substantially identical" or "substantial identity" in the context of two nucleic acids or polypeptides, refers to two or more sequences or sub-sequences that have at least about 60%, 70%, 75%, preferably 80% or 85%, more preferably 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or greater nucleotide or amino acid residue percent identity, respectively, when compared and aligned for maximum correspondence, as measured using one of the following sequence comparison algorithms or by visual inspection. In certain aspects, the substantial identity exists over a region of amino acid sequences of at least about 50 residues in length, such as, at least about 100, 110, 120, 125, 130, 135, 140, 145, 150, 155, 160, or 165 amino acid residues. In certain aspects, substantial identity exists over a region of nucleic acid sequences of at least about 150 nucleic acid residues, such as at least about 200, 250, 300, 330, 360, 375, 400, 425, 450, 460, 480, 500, 600, 700, 800 such as at least about 900 nucleotides or such as at least about 1 kb, 1.1 kb, 1.2 kb, 1.3 kb, 1.4 kb, 1.5 kb, 1.6 kb, 1.7 kb, 1.8 kb, 1.9 kb, 2 kb, 2.1 kb, 2.2 kb, 2.3 kb, 2.4 kb, 2.5 kb, 2.6 kb, 2.7 kb, 2.8 kb, 2.9 kb or such as at least about 3 kb. In some aspects, the amino acid or nucleic acid sequences are substantially identical over the entire length of the polypeptide sequence or the corresponding coding region.

[0060] The term "Conservative substitutions" are within the group of basic amino acids (arginine, lysine and histidine), acidic amino acids (glutamic acid and aspartic acid), polar amino acids (glutamine and asparagine), hydrophobic amino acids (leucine, isoleucine, valine and methionine), aromatic amino acids (phenylalanine, tryptophan and tyrosine), and small amino acids (glycine, alanine, serine and threonine). Amino acid substitutions which do not generally alter the specific activity are known in the art and are described, for example, by Neurath and Hill, 1979. The most commonly occurring exchanges are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr, Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile, Leu/Val, Ala/Glu, and Asp/Gly as well as these in reverse.

[0061] The term "conservatively substituted variant" as used herein refers to a variant of a nucleotide sequence comprising one or more conservative substitutions.

[0062] Generally and in the present context, the term "silent substitution" refers to a base substitution which does not affect the sense of a codon and thus has no effect on polypeptide structure. As the skilled person will know silent substitutions are possible because of the degeneracy of the genetic code.

[0063] The term "conserved domain" refers to a sequence of amino acids in a polypeptide or a sequence of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a consensus sequence. Amino acid motifs are often composed of conserved sequences. Additionally, the term "conserved sequence" refers to a base sequence in a nucleic acid sequence molecule or an amino acid sequence in a protein that has remained essentially unchanged throughout evolution. A "consensus sequence" is defined in terms of an idealized sequence that represents the base most often present at each position in a nucleic acid sequence or the amino acid most often present at each position in a protein. A "consensus sequence" is identified by aligning all known examples of a nucleic acid sequence or a protein so as to maximise their sequence identity. For a sequence to be accepted as a consensus sequence each particular base or amino acid must be reasonably predominant at its position and most of the sequences must be related to the consensus by only few substitutions, such as 1 or 2.

[0064] A homologue may also be in the form of an "insertional variant" of a protein, i.e. where one or more amino acid residues are introduced into a predetermined site in a protein. Insertions may comprise N-terminal and/or C-terminal fusions as well as intra-sequence insertions of single or multiple amino acids. Generally, insertions within the amino acid sequence will be smaller than N- or C-terminal fusions, of the order of about 1 to 10 residues. Examples of N- or C-terminal fusion proteins or peptides include the binding domain or activation domain of a transcriptional activator as used in the yeast two-hybrid system, phage coat proteins, (histidine)-6-tag, glutathione S-transferase-tag, protein A, maltose-binding protein, dihydrofolate reductase, Tag-100 epitope, c-myc epitope, FLAG®-epitope, lacZ, CMP (calmodulin-binding peptide), HA epitope, protein C epitope and VSV epitope.

[0065] Homologues in the form of "deletion variants" of a protein are characterised by the removal of one or more amino acids from a protein.

[0066] Homologues in the form of "addition variants" of a protein are characterised by the addition of one or more amino acids from a protein, whereby the addition may be at the end of the sequence.

[0067] Amino acid variants of a protein may readily be made using peptide synthetic techniques well known in the art, such as solid phase peptide synthesis and the like, or by recombinant DNA manipulations. Methods for the manipulation of DNA sequences to produce substitution, insertion or deletion variants of a protein are well known in the art. For example, techniques for making substitution mutations at predetermined sites in DNA are well known to those skilled in the art and include M13 mutagenesis, T7-Gen in vitro mutagenesis (USB, Cleveland, Ohio), QuickChange Site-Directed mutagenesis (Stratagene, San Diego, Calif.), PCR-mediated site-directed mutagenesis or other site-directed mutagenesis protocols.

[0068] The terms "Orthologs" and "Paralogs"-sequences are also a type of homologous sequences as described above. Several different methods are known by those of skill in the art for identifying and defining these functionally homologous sequences. Three general methods for defining orthologs and paralogs are described; an ortholog, paralog or homolog may be identified by one or more of the methods described below.

[0069] Orthologs and paralogs are evolutionarily related genes that have similar sequence and similar functions. Orthologs are structurally related genes in different species that are derived by a speciation event. Paralogs are structurally related genes within a single species that are derived by a duplication event.

[0070] Within a single plant species, gene duplication may cause two copies of a particular gene, giving rise to two or more genes with similar sequence and often similar function known as paralogs. A paralog is therefore a similar gene formed by duplication within the same species. Paralogs typically cluster together or in the same Glade (a group of similar genes) when a gene family phylogeny is analyzed using programs such as CLUSTAL (Thompson et al.; Higgins et al. Groups of similar genes can also be identified with pair-wise BLAST analysis (Feng and Doolittle. For example, a Glade of very similar MADS domain transcription factors from Arabidopsis all share a common function in flowering time (Ratcliffe et al.), and a group of very similar AP2 domain transcription factors from Arabidopsis are involved in tolerance of plants to freezing (Gilmour et al.). Analysis of groups of similar genes with similar function that fall within one Glade can yield sub-sequences that are particular to the Glade. These sub-sequences, known as consensus sequences, can not only be used to define the sequences within each Glade, but define the functions of these genes; genes within a Glade may contain paralogous sequences, or orthologous sequences that share the same function (see also, for example, Mount (2001), in Bioinformatics: Sequence and Genome Analysis Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., page 543.)

[0071] Speciation, the production of new species from a parental species, can also give rise to two or more genes with similar sequence and similar function. These genes, termed orthologs, often have an identical function within their host plants and are often interchangeable between species without losing function. Because plants have common ancestors, many genes in any plant species will have a corresponding orthologous gene in another plant species. Once a phylogenic tree for a gene family of one species has been constructed using a program such as CLUSTAL potential orthologous sequences can be placed into the phylogenetic tree and their relationship to genes from the species of interest can be determined. Orthologous sequences can also be identified by a reciprocal BLAST strategy. Once an orthologous sequence has been identified, the function of the ortholog can be deduced from the identified function of the reference sequence.

[0072] Orthologous genes from different organisms have highly conserved functions, and very often essentially identical functions (Lee et al. and Remm et al.). Paralogous genes, which have diverged through gene duplication, may retain similar functions of the encoded proteins. In such cases, paralogs can be used interchangeably with respect to certain embodiments of the instant invention (for example, transgenic expression of a coding sequence). An example of such highly related paralogs is the CBF family, with three well-defined members in Arabidopsis and at least one ortholog in Brassica napus, all of which control pathways involved in both freezing and drought stress (Gilmour et al. and Jaglo et al.)

[0073] The following references represent a small sampling of the many studies that demonstrate that conserved transcription factor genes from diverse species are likely to function similarly (i.e., regulate similar target sequences and control the same traits), and that transcription factors may be transformed into diverse species to confer or improve traits.

[0074] (1) The Arabidopsis NPR1 gene regulates systemic acquired resistance (SAR); over-expression of NPR1 leads to enhanced resistance in Arabidopsis. When either Arabidopsis NPR1 or the rice NPR1 ortholog was overexpressed in rice (which, as a monocot, is diverse from Arabidopsis), challenge with the rice bacterial blight pathogen Xanthomonas oryzae pv. Oryzae, the transgenic plants displayed enhanced resistance (Chern et al.). NPR1 acts through activation of expression of transcription factor genes, such as TGA2 (Fan and Dong).

[0075] (2) E2F genes are involved in transcription of plant genes for proliferating cell nuclear antigen (PCNA). Plant E2Fs share a high degree of similarity in amino acid sequence between monocots and dicots, and are even similar to the conserved domains of the animal E2Fs. Such conservation indicates a functional similarity between plant and animal E2Fs. E2F transcription factors that regulate meristem development act through common cis-elements, and regulate related (PCNA) genes (Kosugi and Ohashi).

[0076] The term "closely related" genes is used for genes that are orthologous or paralogous.

[0077] The term "promoter," as used herein, refers to a region of sequence determinants located upstream from the start of transcription of a gene and which are involved in recognition and binding of RNA polymerase and other proteins to initiate and modulate transcription. Promoters useful in plants need not be of plant origin. A "basal promoter" is the minimal sequence necessary for assembly of a transcription complex required for transcription initiation. Basal promoters frequently include a TATA box" element usually located between 15 and 35 nucleotides upstream from the site of initiation of transcription. Basal promoters also sometimes include a CCAAT box" element (typically a sequence CCAAT) and/or a GGGCG sequence, usually located between 40 and 200 nucleotides, preferably 60 to 120 nucleotides, upstream from the start site of transcription.

[0078] Promoters referred to herein as "constitutive promoters" actively promote transcription under most, but not necessarily all, environmental conditions and states of development or cell differentiation. Examples of constitutive promoters include the cauliflower mosaic virus (CaMV) 35S transcript initiation region and the 1' or 2' promoter derived from T-DNA of Agrobacterium tumefaciens, and other transcription initiation regions from various plant genes, such as the maize ubiquitin-1 promoter, known to those of skill. Organ-specific promoters may be, for example, a promoter from storage sink tissues such as seeds, potato tubers, and fruits, or from metabolic sink tissues such as meristems, a seed specific promoter such as the glutelin, prolamin, globulin, or albumin promoter from rice, a Vicia faba promoter from the legumin B4 and the unknown seed protein gene from Vicia faba, a promoter from a seed oil body protein, the storage protein napA promoter from Brassica napus, or any other seed specific promoter known in the art, e.g., as described in WO 91/14772. Furthermore, the promoter may be a leaf specific promoter such as the rbcs promoter from rice or tomato, the chlorella virus adenine methyltransferase gene promoter, or the aldP gene promoter from rice, or a wound inducible promoter such as the potato pin2 promoter.

[0079] An "inducible promoter" in the context of the present invention refers to a promoter which is regulated under certain conditions, such as light, chemical concentration, protein concentration, conditions in an organism, cell, or organelle, etc. An example of an inducible promoter is the HSP promoter and the PARSK1, the promoter from the Arabidopsis gene encoding a serine-threonine kinase enzyme and which is induced by dehydration, abscissic acid and sodium chloride. In essence, expression under the control of an inducible promoter is "switched on" or increased in response to an applied stimulus. The nature of the stimulus varies between promoters and may include the above environmental factors. Whatever the level of expression is in the absence of the stimulus, expression from any inducible promoter is increased in the presence of the correct stimulus.

[0080] As used herein, the term "tissue specific" refers to a characteristic of a particular tissue that is not generally found in all tissues, or may be exclusive found in a tissue of interest. In the present application, "tissue specific" is used in reference to a gene regulatory element (promoter or promoter plus enhancer and/or silencer), the gene it encodes, or the polypeptide product of such a gene. In the context of a gene regulatory element or a "tissue specific promoter", the term means that the promoter (and also other regulatory elements such as enhancer and/or silencer elements) directs the transcription of a linked sequence in a cell of a particular lineage, tissue, or cell type, but is substantially inactive in cells or tissues not of that lineage, tissue, or cell type. A tissue specific promoter useful according to the invention is at least 5-fold, 10-fold, 25-fold, 50 fold, 100-fold, 500-fold or even 1,000 times more active in terms of transcript production in the particular tissue than it is in cells of other tissues or in transformed or malignant cells of the same lineage. In the context of a gene or the polypeptide product of a gene, the term tissue specific means that the polypeptide product of the gene is detectable in cells of that particular tissue or cell type, but not substantially detectable in certain other cell types. Particularly relevant tissue specific promoters include promoter sequences specifically expressed or active in the xylem forming tissue in a plant. Examples of such promoters are the Lmp1, Lmx2, Lmx3, Lmx4 and Lmx5 promoters, described in WO2004097024.

[0081] A "terminator sequence" refers to a section of genetic sequence that marks the end of gene or operon on genomic DNA for transcription. Terminator sequences are recognized by protein factors that co-transcriptionally cleave the nascent RNA at a polyadenylation signal, halting further elongation of the transcript by RNA polymerase. A nucleic acid is "operably linked" when it is placed into a functional relationship with another nucleic acid sequence. For instance, a promoter or enhancer is operably linked to a coding sequence if it increases the transcription of the coding sequence. Operably linked means that the DNA sequences being linked are typically contiguous and, where necessary to join two protein coding regions, contiguous and in reading frame. However, since enhancers generally function when separated from the promoter by several kilobases and intronic sequences may be of variable lengths, some polynucleotide elements may be operably linked but not contiguous.

[0082] In the context of the present invention the terms "transformation" and "transforming" are used interchangeably and as synonyms to "transfecting" and "transfection", respectively, to refer to the process of introducing DNA into a cell. The DNA constructs, including at least a portion of the gene or promoter of interest, can be introduced into host cells, which as stated previously, can be individual cells, cells in culture, cells as part of a host organism, a fertilized oocyte orgametophyte or an embryonic cell. By the term "introduction" when used in reference to a host cell is meant to refer to standard procedures known in the art for introducing recombinant vector DNA into the target host cell. Such procedures include, but are not limited to, transfection, infection, transformation, natural uptake, electroporation, biolistics and Agrobacterium.

[0083] By "regenerable cell" is meant a plant cell from which a whole plant can be regenerated. It will be understood that the regenerable cell is a cell that has maintained its genetic potential, also known in the art as "totipotency". It will further be understood that the regenerable cells, when grown in culture, may need the appropriate stimuli to express the total genetic potential of the parent plant.

Method of Producing a Transgenic Plant

[0084] In specific embodiments of the invention advantageous plant phenotypes are generated by modifying, relative to the corresponding wild-type plant, the expression level of candidate genes that have been evaluated and selected according to the above criteria. According to these aspects a method is provided which comprises altering in the plant the level of a gene product of at least one gene comprising a nucleotide sequence selected from the group consisting of:

[0085] a) a nucleotide sequence from SEQ ID NO: 1-13, 97-115;

[0086] b) a nucleotide sequence being at least 60% identical to a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0087] c) a subsequence or fragment of a nucleotide sequence of a) or b).

[0088] This may be done by following technically modified crossing method comprising

[0089] i) selecting plant species expressing at least one of the nucleotide sequences selected from the group consisting of

[0090] a) a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0091] b) a nucleotide sequence being at least 60% identical to a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0092] c) a subsequence or fragment of a nucleotide sequence of a) or b,

[0093] ii) crossing a plant species selected in i) with the same or another plant species selected in i),

[0094] iii) selecting plants with modulated expression of at least one of the nucleotide sequences selected from the group consisting of

[0095] a) nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0096] b) a nucleotide sequence being at least 60% identical to a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0097] c) a subsequence or fragment of a nucleotide sequence of a) or b. compared to the plant species selected under i)

[0098] iv) optionally backcrossing one or more times the plants obtained in iii) and selecting plants with modulated expression of at least one of the nucleotide sequences selected from the group consisting of

[0099] a) a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0100] b) a nucleotide sequence being at least 60% identical to a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0101] c) a subsequence or fragment of a nucleotide sequence of a) or b) compared to any of the plant species used in i) and/or plants obtained in iii).

[0102] According to one aspect of the invention a method is provided comprising the following steps:

[0103] (i) providing an expression vector comprising a nucleotide sequence selected from the group consisting of

[0104] a) a nucleotide sequence from SEQ ID NO 1-13, 97-115; or

[0105] b) a nucleotide sequence being at least 60% identical to a nucleotide sequence from SEQ ID NO 1-13, 97-115; or

[0106] c) a subsequence or fragment of a nucleotide sequence of a) or b) and

[0107] d) at least one regulatory element operably linked to the polynucleotide sequence, wherein said at least one regulatory element controls expression of the polynucleotide sequence in a target plant;

[0108] (ii) introducing the expression vector into at least one plant; and

[0109] (iii) selecting at least one transgenic plant that has a modulated growth and/or biomass compared to its wild type.

[0110] The sequences specified by sequence ID numbers 1-13, 97-115; represent sequences of the candidate genes as predicted from Populus trichocarpa and seq ID numbers 73-95 as cloned from hybrid aspen. As the skilled person will understand, additional sequence from these genes 5' as well as 3' to the sequence described in SEQ ID NOs: 73-95 is readily achievable using conventional cloning techniques, such as those described in Sambrook et al.

[0111] According to one embodiment the modulated expression is effected by introducing a genetic modification preferably in the locus of a gene encoding a polypeptide comprising SEQ ID NO: 1-13, 97-115 or a homologue of such polypeptide.

[0112] The modification nay be effected by one of: T-DNA activation, TILLING, homologous recombination, site-directed mutagenesis or directed breeding using one or more of SEQ ID NO: 1-13, 97-115 as markers in any step of the process.

[0113] The effect of the modulation may be increased yield in growth and/or in biomass.

Nucleic Acid Constructs

[0114] According to more particular embodiments of the invention, the method comprises the step of providing a nucleic acid construct, such as a recombinant DNA construct, comprising a nucleotide sequence selected from the group consisting of:

[0115] a) a nucleotide sequence comprising a sequence selected from SEQ ID NO: 1-13, 97-115;

[0116] b) a complementary nucleotide sequence of a nucleotide sequence of a);

[0117] c) a sub-sequence or fragment of a nucleotide sequence of b) or c);

[0118] d) a nucleic acid sequence being at least 60% identical to any one of the sequences in a), b) and c); and

[0119] e) a nucleotide sequence which hybridizes under stringent conditions to a nucleotide sequence of a), b) or c).

[0120] In further embodiments of the invention the nucleic acid sequence in c) or g) is at least 65% identical to any one of the sequences in a), c), d), e) or f), such as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to any one of the sequences in a), c), d), e) or f).

[0121] In preferred embodiments of this aspect of the invention the nucleotide sequence of a) is selected from the group consisting of SEQ ID NOs: 1, 4, 6, 7, 9, 10, 101, 102, 104, 106 and 107.

[0122] A variety of methods exist in the art for producing the nucleic acid sequences and nucleic acid/DNA constructs of the invention. Procedures for identifying and isolating DNA clones are well known to those of skill in the art, and are described in, e.g. Sambrook et al., Molecular Cloning--A Laboratory Manual (2nd Ed.), Vol. 1-3, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989. Alternatively, the nucleic acid sequences of the invention can be produced by a variety of in vitro amplification methods adapted to the present invention by appropriate selection of specific or degenerate primers. Examples of protocols sufficient to direct persons of skill through in vitro amplification methods, including the polymerase chain reaction (PCR) the ligase chain reaction (LCR), Qbeta-replicase amplification and other RNA polymerase mediated techniques (e.g., NASBA), e.g., for the production of the homologous nucleic acids of the invention are found in Sambrook, supra.

[0123] Alternatively, nucleic acid constructs of the invention can be assembled from fragments produced by solid-phase synthesis methods. Typically, fragments of up to approximately 100 bases are individually synthesized and then enzymatically or chemically ligated to produce a desired sequence, e.g., a polynucletotide encoding all or part of a transcription factor. For example, chemical synthesis using the phosphoramidite method is well known to the skilled person. According to such methods, oligonucleotides are synthesized, purified, annealed to their complementary strand, ligated and then optionally cloned into suitable vectors. The invention also relates to vectors comprising the DNA constructs.

[0124] As mentioned, the above described sequences are from hybrid aspen and Populus trichocarpa. As the skilled person will understand, homologues of the described sequences may be isolated from other species, non-limiting examples of which include acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, hickory, birch, chestnut, alder, maple, sycamore, ginkgo, palm tree, sweet gum, cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, pine, redwood, spruce and yew, apple, plum, pear, banana, orange, kiwi, lemon, cherry, grapevine, fig, cotton, bamboo, switch grass, red canary grass and rubber plants. Useful homologues of the described sequences may also be isolated from hardwood plants from the Salicaceae family, e.g. from the salix and populus genus. Members of this genus are known by their common names: willow, poplar and aspen.

[0125] Examples of other suitable plants for use in accordance with any aspect of the invention described herein include monocotyledons, dicotelydons, gymnosperms and algae, ferns and mosses. Of particular interest are transgenic higher plants, especially agricultural crops, for example cereals, and flowers, which have been engineered to carry a heterologous nucleic acid as described above, including tobacco, cucurbits, carrot, vegetable brassica, melons, capsicums, grape vines, lettuce, strawberry, oilseed brassica, sugar beet, wheat, barley, maize, rice, sugar cane, soybeans, peas, sorghum, sunflower, tomato, potato, pepper, chrysanthemum, carnation, linseed, hemp and rye.

[0126] In some preferred embodiments, the plant is a perennial plant, for example a woody perennial plant. A woody perennial plant is a plant which has a life cycle which takes longer than 2 years and involves a long juvenile period in which only vegetative growth occurs. This is contrasted with an annual or herbaceous plant such as Arabidopsis thaliana or Lycopersicon esculentum (tomato), which have a life cycle which is completed in one year.

[0127] In particular, the method according to the present invention may comprise a step of providing a nucleic acid construct, such as a recombinant DNA construct, comprising a nucleotide sequence which relative to the particular sequences described, comprises conservative variations altering only one, or a few amino acids in the encoded polypeptide may also be provided and used according to the present invention. Accordingly, it is within the scope of the invention to provide and use a recombinant DNA construct comprising a nucleotide sequence which encodes a polypeptide comprising a conservatively substituted variant of a polypeptide of a).

[0128] Sequence alterations that do not change the amino acid sequence encoded by the polynucleotide are termed "silent" substitutions. With the exception of the codons ATG and TGG, encoding methionine and tryptophan, respectively, any of the possible codons for the same amino acid can be substituted by a variety of techniques, e.g., site-directed mutagenesis, available in the art. Accordingly, the present invention may also provide a recombinant nucleic acid construct, wherein the nucleotide sequence comprises a silent substitution in a nucleotide sequence.

[0129] In certain further embodiments of the invention, the sub-sequences or fragments have at least 65% sequence identity to a conserved domain of a nucleotide sequence as described above under item a) or d), such as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to a conserved domain of a nucleotide sequence as described above under item a) or d).

[0130] Thus, there are methods for identifying a sequence similar or paralogous or orthologous or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an Internet or intranet) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.

Approaches to Obtaining Altering the Level of a Gene Product

[0131] This invention is used by increasing the expression of certain genes, non limiting examples how this can be done are presented here. The nucleic acid construct or recombinant DNA construct as described above may be used for the identification of plants having altered growth characteristics as compared to the wild-type. Such plants may for instance be naturally occurring variants or plants that have been modified genetically to exhibit altered growth properties. For such purposes the nucleic acid construct or recombinant DNA construct according to the invention may be used e.g. as a probe in conventional hybridization assays or as a primer for specific amplification of nucleic acid fragments.

[0132] Although the main part of this invention is how an up regulation of the gene products gives the desired effect. It also shows that changing the expression of the genes presented here can be used to modify the desired properties, this is another way to look at the data, and an effect of this view is that also decreasing the gene products within the plant is a way to modify the desired trait. There are different ways to increase the levels of a gene product, these are described below in parallel with the ways to up-regulate a gene product.

[0133] One of the genes SEQ ID NO:1-13, 97-115; could also be used as targets for marker assisted breeding because changes in the gene regulatory sequences can give changes in the expression patterns and changes in the coding sequences can give changes in the gene function, and we have shown that manipulating these genes gives changes in the desired traits. This is usually referred to that the genes SEQ ID No 1-13, 97-115; can be used as candidate genes Brady and Provart 2007, and Varshney et al 2005

[0134] One particular way to use this invention is to measure the expression of one or more of the genes SEQ ID NO:1-13, 97-115; using for example quantitative RT-PCR in natural populations and select for unusual high expression of the measured gene and use such plants as parents in a breeding program, this could be repeated for each breeding cycle. Methods to quantify gene expression, including real time PCR, are described in Sambrook et al.

[0135] The genes presented here can also be used in candidate gene-based association studies, the result from such studies can then be used in marker assisted breeding. Burke et al 2007.

[0136] Up regulation or over expression of a gene can be achieved by placing the full open reading frame of the gene behind a suitable promoter, which are described elsewhere, and usually placing terminator and poly-adenylation signal sequence 3' of the gene to be over expressed.

[0137] In addition, the nucleic acid construct or recombinant DNA construct according to the invention may be used for the purpose of gene replacement in order to modify the plant growth phenotype.

[0138] Suppression of endogenous gene expression can for instance be achieved using a ribozyme. Ribozymes are RNA molecules that possess highly specific endoribonuclease activity. The production and use of ribozymes are disclosed in U.S. Pat. No. 4,987,071 and U.S. Pat. No. 5,543,508. While antisense techniques are discussed below, it should be mentioned that synthetic ribozyme sequences including antisense RNAs can be used to confer RNA cleaving activity on the antisense RNA, such that endogenous mRNA molecules that hybridize to the antisense RNA are cleaved, which in turn leads to an enhanced antisense inhibition of endogenous gene expression.

[0139] Vectors in which RNA encoded by a relevant gene homologue is over-expressed can also be used to obtain co-suppression of a corresponding endogenous gene, e.g., in the manner described in U.S. Pat. No. 5,231,020 to Jorgensen. Such co-suppression (also termed sense suppression) does not require that the entire gene sequence be introduced into the plant cells, nor does it require that the introduced sequence be exactly identical to the endogenous sequence of interest. However, the suppressive efficiency will be enhanced as specificity of hybridization is increased, e.g., as the introduced sequence is lengthened, and/or as the sequence similarity between the introduced sequence and the endogenous transcription factor gene is increased.

[0140] Vectors expressing an untranslatable form of gene, e.g., sequences comprising one or more stop codons, or nonsense mutation, can also be used to suppress expression of an endogenous transcription factor, thereby reducing or eliminating it's activity and modifying one or more traits. Methods for producing such constructs are described in U.S. Pat. No. 5,583,021. In particular, such constructs can be made by introducing a premature stop codon into the gene.

[0141] One way of performing targeted DNA insertion is by use of the retrovirus DNA integration machinery as described in WO2006078431. This technology is based on the possibility of altering the integration site specificity of retroviruses and retrotransposons integrase by operatively coupling the integrase to a DNA-binding protein (tethering protein). Engineering of the integrase is preferably carried out on the nucleic acid level, via modification of the wild type coding sequence of the integrase by PCR. The integrase complex may thus be directed to a desired portion or be directed away from an undesired portion of genomic DNA thereby producing a desired integration site characteristic.

[0142] Another technology that can be used to alter and preferably, in this invention, increase gene expression is the "Targeting Induced Local Lesions in Genomes", which is a non-transgenic way to alter gene function in a targeted way. This approach involves mutating a plant with foe example ethyl methanesulfonate (EMS) and later locating the individuals in which a particular desired gene has been modified. The technology is described for instance in Slade and Knauf, 2005 and Henikoff, et al.

[0143] A method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in an appropriate gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation.

[0144] As will be apparent to the skilled person, a plant trait can also be modified by using the cre-lox system. A plant genome can be modified to include first and second lox sites that are then contacted with a Cre recombinase. Provided that the lox sites are in the same orientation, the intervening DNA sequence between the two sites will be excised. If the lox sites are in the opposite orientation, the intervening sequence is inverted.

[0145] The polynucleotides and polypeptides of this invention can also be expressed in a plant in the absence of an expression cassette by manipulating the activity or expression level of the endogenous gene by other means, for example, by ectopically expressing a gene by T-DNA activation tagging, Ichikawa et al. (1997); Kakimoto et al. (1996). This method entails transforming a plant with a gene tag containing multiple transcriptional enhancers and once the tag has inserted into the genome, expression of a flanking gene coding sequence becomes deregulated. In another example, the transcriptional machinery in a plant can be modified so as to increase transcription levels of a polynucleotide of the invention (See, e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe the modification of the DNA binding specificity of zinc finger proteins by changing particular amino acids in the DNA binding motif).

Antisense Suppression of Expression

[0146] However, the recombinant DNA construct, comprising a nucleotide sequence as described above is particularly useful for sense and anti-sense suppression of expression, e.g., to down-regulate expression of a particular gene, in order to obtain a plant phenotype with increased growth. That is, the nucleotide sequence of the invention, or sub-sequences or anti-sense sequences thereof, can be used to block expression of naturally occurring homologous nucleic acids. Varieties of traditional sense and antisense technologies are known in the art, e.g., as set forth in Lichtenstein and Nellen (1997). The objective of the antisense approach is to use a sequence complementary to the target gene to block its expression and create a mutant cell line or organism in which the level of a single chosen protein is selectively reduced or abolished.

[0147] For more elaborate descriptions of anti-sense regulation of gene expression as applied in plant cells reference is made to U.S. Pat. No. 5,107,065, the content of which is incorporated herein in its entirety.

RNA Interference

[0148] Gene silencing that is induced by double-stranded RNA is commonly called RNA interference or RNAi. RNA interference is a molecular mechanism in which fragments of double-stranded ribonucleic acid (dsRNA) interfere with the expression of a particular gene that shares a homologous sequence with the dsRNA. The process that is mediated by the same cellular machinery that processes microRNA, known as the RNA-induced silencing complex (RISC). The process is initiated by the ribonuclease protein Dicer, which binds and cleaves exogenous double-stranded RNA molecules to produce double-stranded fragments of 20-25 base pairs with a few unpaired overhang bases on each end. The short double-stranded fragments produced by Dicer, called small interfering RNAs (siRNAs), are separated and integrated into the active RISC complex. If one part of an RNA transcript is targeted by an RNAi molecule or construct, the whole transcript is down-regulated.

[0149] For more elaborate descriptions of RNAi gene suppression in plants by transcription of a dsRNA reference is made to U.S. Pat. No. 6,506,559, US 2002/0168707, and WO 98/53083, WO 99/53050 and WO 99/61631, all of which are incorporated herein by reference in their entirety.

Construction of Vectors

[0150] In general, those skilled in the art are well able to construct vectors of the present invention and design protocols for recombinant gene expression. For further details on general protocols for preparation of vectors reference is made to: Molecular Cloning: a Laboratory Manual: 2nd edition, Sambrook et al, 1989, Cold Spring Harbor Laboratory Press. The promoter used for the gene may influence the level, timing, tissue, specificity, or inducibility of the over expression.

[0151] Generally, over expression of a gene can be achieved using a recombinant DNA construct having a promoter operably linked to a DNA element comprising a sense element of a segment of genomic DNA or cDNA of the gene, e.g., the segment should contain enough of the open reading frame to produce a functional protein and preferably the full open reading frame.

[0152] In pertinent embodiments of the invention the nucleic acid construct, or recombinant DNA construct, further comprising a constitutive, inducible, or tissue specific promoter operably linked to said nucleotide sequence.

[0153] In a presently preferred embodiment of the invention, the nucleic acid construct, or recombinant DNA construct, comprises the sequence of SEQ ID NO: 96 the vector.

[0154] The presently preferred nucleic acid construct for over expression, is a vector termed pK2GW7. The vector is described in: Gateway vectors for Agrobacterium-mediated plants transformation, Karimi, 2002.

Transformation of Plant Cells

[0155] In accordance with the present invention, the method comprise the further step of transforming regenerable cells of a plant with said nucleic acid construct or recombinant DNA construct and regenerating a transgenic plant from said transformed cell. When introducing the above DNA construct or vector into a plant cell, certain considerations must be taken into account, well known to those skilled in the art. The nucleic acid to be inserted should be assembled within a construct that contains effective regulatory elements that will drive transcription, as described above. There must be available a method of transporting the construct into the cell. Once the construct is within the cell, integration into the endogenous chromosomal material either will or will not occur.

[0156] Transformation techniques, well known to those skilled in the art, may be used to introduce the DNA constructs and vectors into plant cells to produce transgenic plants, in particular transgenic trees, with improved plant growth.

[0157] A person of skills in the art will realise that a wide variety of host cells may be employed as recipients for the DNA constructs and vectors according to the invention. Non-limiting examples of host cells include cells in embryonic tissue, callus tissue type I, II, and III, hypocotyls, meristem, root tissue, tissues for expression in phloem.

[0158] As listed above, Agrobacterium transformation is one method widely used by those skilled in the art to transform tree species, in particular hardwood species such as poplar. Production of stable, fertile transgenic plants is now a routine in the art. Other methods, such as microprojectile or particle bombardment, electroporation, microinjection, direct DNA uptake, liposome mediated DNA uptake, or the vortexing method may be used where Agrobacterium transformation is inefficient or ineffective, for example in some gymnosperm species.

[0159] Alternatively, a combination of different techniques may be employed to enhance the efficiency of the transformation process, e.g. bombardment with Agrobacterium coated microparticles or microprojectile bombardment to induce wounding followed by co-cultivation with Agrobacterium.

[0160] It will be understood, that the particular choice of a transformation technology will be determined by its efficiency to transform certain plant species as well as the experience and preference of the person practising the invention with a particular methodology of choice. It will be apparent to the skilled person that the particular choice of a transformation system to introduce nucleic acid into plant cells is not essential to or a limitation of the invention, nor is the choice of technique for plant regeneration.

[0161] Following transformation, transgenic plants are preferably selected using a dominant selectable marker incorporated into the transformation vector. Typically, such a marker will confer antibiotic or herbicide resistance on the transformed plants and selection of transformants can be accomplished by exposing the plants to appropriate concentrations of the antibiotic or herbicide. A novel selection marker using the D-form of amino acids and based on the fact that plants can only tolerate the L-form offers a fast, efficient and environmentally friendly selection system. An interesting feature of this selection system is that it enables both selection and counter-selection.

[0162] Subsequently, a plant may be regenerated, e.g. from single cells, callus tissue or leaf discs, as is standard in the art. Almost any plant can be entirely regenerated from cells, tissues and organs of the plant. Available techniques are reviewed in Vasil et al. 1984.

[0163] After transformed plants are selected and grown to maturity, those plants showing an increase growth phenotype are identified. Additionally, to confirm that the phenotype is due to changes in expression levels or activity of the polypeptide or polynucleotide disclosed herein can be determined by analyzing mRNA expression using Northern blots, RT-PCR or microarrays, or protein expression using immunoblots or Western blots or gel shift assays.

Plant Species

[0164] In accordance with the invention, the present method produces a transgenic plant having an increased growth compared to its wild type plant from which it is derived. In an embodiment of the present method, the transgenic plant is a perennial plant, i.e. a plant that lives for more than two years. In a specific embodiment, the perennial plant is a woody plant which may be defined as a vascular plant that has a stem (or more than one stem) which is lignified to a high degree.

[0165] In a preferred embodiment, the woody plant is a hardwood plant, i.e. broad-leaved or angiosperm trees, which may be selected from the group consisting of acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, willow, hickory, birch, chestnut, poplar, alder, maple, sycamore, ginkgo, palm tree and sweet gum. Hardwood plants from the Salicaceae family, such as willow, poplar and aspen, including variants thereof, are of particular interest, as these two groups include fast-growing species of tree or woody shrub which are grown specifically to provide timber and bio-fuel for heating. Cellulosic grasses used for bioenergy like Switch grass and Red Canary Grass are also interesting.

[0166] In further embodiments, the woody plant is softwood or a conifer which may be selected from the group consisting of cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, pine, redwood, spruce and yew.

[0167] In useful embodiments, the woody plant is a fruit bearing plant which may be selected from the group consisting of apple, plum, pear, banana, orange, kiwi, lemon, cherry, grapevine and FIG.

[0168] Other woody plants which may be useful in the present method may also be selected from the group consisting of cotton, bamboo and rubber plants.

DNA Construct

[0169] According to a second main aspect of the invention a DNA construct, such as a recombinant DNA construct, is provided comprising at least one sequence as described above. In particular, the recombinant DNA construct may comprise a nucleotide sequence selected from the group consisting of:

[0170] a) a nucleotide sequence comprising a sequence selected from SEQ ID NO: 1-13, 97-115;

[0171] b) a complementary nucleotide sequence of a nucleotide sequence of a);

[0172] c) a sub-sequence or fragment of a nucleotide sequence of a) or b);

[0173] d) a nucleic acid sequence being at least 60% identical to any one of the sequences in a), b) and c); and

[0174] e) a nucleotide sequence which hybridizes under stringent conditions to a nucleotide sequence of a), b) or c).

[0175] In selected embodiments of the invention the nucleic acid sequence in d) is at least 65% identical to any one of the sequences in a), b) and c), such as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to any one of the sequences in a), b) and c).

[0176] Also, in accordance with the discussion above, the nucleotide sequence encodes a polypeptide comprising a conservatively substituted variant of a polypeptide of (a). Further, the nucleotide sequence comprises a silent substitution in a nucleotide sequence.

[0177] In additional embodiments of the pertaining to this aspect of the invention, the sub-sequences or fragments have at least 65% sequence identity to a conserved domain of a nucleotide sequence as described above under item a). such as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to a conserved domain of a nucleotide sequence as described above under item a).

[0178] In further embodiments and in accordance with the description above, the recombinant DNA construct further comprising a constitutive, inducible, or tissue specific promoter operably linked to said nucleotide sequence. In particular, the recombinant DNA construct may further comprise a strong constitutive promoter in front of a transcribed cassette consisting of the full open reading frame of the gene followed by an terminator sequence. Such a cassette may comprise a nucleotide sequence as defined in claim 7 and on page 21 and the paragraph bridging pages 21 and 22.

[0179] In the presently exemplified embodiments of the invention the recombinant DNA construct comprises the sequence of SEQ ID NO: 96.

Transgenic Plants

[0180] A third aspect of the invention provides a transgenic plant comprising a recombinant polynucleotide (DNA construct) comprising a nucleotide sequence capable of altering in the plant the level of a gene product of at least one of the genes SEQ ID 1-13, 97-115; Giving increased growth when comparing said group of transgenic plants grown for 8 weeks in a greenhouse under a photoperiod of 18 hours, a temperature of 22° C./15° C. (day/night) and a weekly fertilization with N 84 g/l, PI 2 g/l, K 56 g/l, with a group of wild-type plants grown under identical conditions;

[0181] According to particular embodiments of the invention the level of a gene product of at least one gene comprising a nucleotide sequence selected from the group consisting of:

[0182] a) a nucleotide sequence from SEQ ID NO: 1-13, 97-115;

[0183] b) a nucleotide sequence being at least 60% identical to a nucleotide sequence from SEQ ID NO 1-13, 97-115;

[0184] c) a subsequence or fragment of a nucleotide sequence of a) or b) has been altered relative to the level found in the respective corresponding wild-type plant.

[0185] According to yet another embodiment of the invention, the transgenic plant comprises a recombinant polynucleotide (DNA construct) comprising a nucleotide sequence selected from the group consisting of:

[0186] a) a nucleotide sequence comprising a sequence selected from SEQ ID NO: 1-13, 97-115;

[0187] b) a complementary nucleotide sequence of a nucleotide sequence of a);

[0188] c) a sub-sequence or fragment of a nucleotide sequence of a) or b);

[0189] d) a nucleic acid sequence being at least 60% identical to any one of the sequences in a), b) and c); and

[0190] e) a nucleotide sequence which hybridizes under stringent conditions to a nucleotide sequence of a), b) or c).

[0191] In further embodiments of this aspect of the invention the nucleic acid sequence in c) or g) is at least 65% identical to any one of the sequences in a), b), c), d) or e), such as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to any one of the sequences in a), b), c), d) or e). The transgenic plant may also comprise a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of a) or b). The nucleotide sequence may comprise a silent substitution in a nucleotide sequence. Further, sub-sequences or fragments may have at least 65% sequence identity to a conserved domain.

[0192] As mentioned above the skilled person will realize that a variety of methods exist in the art for producing the nucleic acid sequences and polynucleotide constructs of the invention, e.g. by cloning techniques, assembly of fragments generated by solid phase synthesis. Again, the skilled person will understand, homologues of the described sequences may be isolated from other species, non-limiting examples of which include acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, hickory, birch, chestnut, alder, maple, sycamore, ginkgo, palm tree, sweet gum, cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, pine, redwood, spruce and yew, apple, plum, pear, banana, orange, kiwi, lemon, cherry, grapevine, fig, cotton, bamboo, switchgrass, red canary grass and rubber plants. Useful homologues of the described sequences may also be isolated from hardwood plants from the Salicaceae family, such as from willow, poplar or aspen.

[0193] In particular, the transgenic plant according to the present invention may comprise a recombinant DNA construct comprising a nucleotide sequence which relative to the particular sequences described, comprises conservative variations altering only one, or a few amino acids in the encoded polypeptide may also be provided and used according to the present invention. Accordingly, it is within the scope of the invention to provide a transgenic plant comprising a recombinant DNA construct comprising a nucleotide sequence which encodes a polypeptide comprising a conservatively substituted variant of a polypeptide of a) or d).

[0194] Accordingly, the present invention may also provide a recombinant DNA construct, wherein the nucleotide sequence comprises a silent substitution in a nucleotide sequence, that is, the recombinant DNA construct may comprise a sequence alteration that does not change the amino acid sequence encoded by the polynucleotide.

[0195] In certain further embodiments of the invention, the sub-sequences or fragments have at least 65% sequence identity to a conserved domain of a nucleotide sequence as described above under item a) or d), such as at least 70% identical, at least 75% identical, at least 80% identical, at least 85% identical, at least 87% identical, at least 90% identical, at least 95% identical, at least 97% identical, at least 98% identical, at least 99% identical, or at least 99.5% identical to a conserved domain of a nucleotide sequence as described above under item a) or d).

[0196] In the particular embodiments by which the present invention is exemplified the sub-sequences or fragments in c) comprise the sequences of SEQ ID NOs: 18-34.

[0197] In further embodiments the transgenic plant provided according to the invention comprises a recombinant polynucleotide construct which further comprises a constitutive, inducible, or tissue specific promoter operably linked to said nucleotide sequence.

[0198] In still further embodiments the recombinant polynucleotide construct further comprises a strong constitutive promoter in front of a transcribed cassette. The cassette may comprise a nucleotide sequence, wherein modulated expression is effected by introducing a genetic modification preferably in the locus of a gene encoding a polypeptide comprising SEQ ID NO: 1-13, 97-115 or a homologue of such polypeptide followed by a plant functional intron followed by a nucleotide sequence encoding a polypeptide comprising a conservatively substituted variant of a polypeptide of a) or d). in reverse orientation.

[0199] In a presently preferred embodiment of the invention, the transgenic plant according to the invention comprises a recombinant DNA construct comprising the sequence of SEQ ID NO: 96

Plant Species

[0200] In accordance with the present invention, the transgenic plant may be a perennial plant which preferable is a woody plant or a woody species. In a useful embodiment, the woody plant is a hardwood plant which may be selected from the group consisting of acacia, eucalyptus, hornbeam, beech, mahogany, walnut, oak, ash, willow, hickory, birch, chestnut, poplar, alder, maple, sycamore, ginkgo, a palm tree and sweet gum. Hardwood plants from the Salicaceae family, such as willow, poplar and aspen including variants thereof, are of particular interest, as these two groups include fast-growing species of tree or woody shrub which are grown specifically to provide timber and bio-fuel for heating.

[0201] In further embodiments, the woody plant is a conifer which may be selected from the group consisting of cypress, Douglas fir, fir, sequoia, hemlock, cedar, juniper, larch, pine, redwood, spruce and yew.

[0202] In useful embodiments, the woody plant is a fruit bearing plant which may be selected from the group consisting of apple, plum, pear, banana, orange, kiwi, lemon, cherry, grapevine and fig.

[0203] Other woody plants which may be useful in the present method may also be selected from the group consisting of cotton, bamboo and rubber plants.

[0204] The present invention extends to any plant cell of the above transgenic plants obtained by the methods described herein, and to all plant parts, including harvestable parts of a plant, seeds and propagules thereof, and plant explant or plant tissue. The present invention also encompasses a plant, a part thereof, a plant cell or a plant progeny comprising a DNA construct according to the invention. The present invention extends further to encompass the progeny of a primary transformed or transfected cell, tissue, organ or whole plant that has been produced by any of the aforementioned methods, the only requirement being that progeny exhibit the same genotypic and/or phenotypic characteristic(s) as those produced in the parent by the methods according to the invention.

[0205] It should be noted that embodiments and features described in the context of one of the aspects of the present invention also apply to the other aspects of the invention. Thus, definitions of one embodiment regard mutatis mutandis to all other embodiments comprising or relating to the one embodiment. When for example definitions are made regarding DNA constructs or sequences, such definitions also regard e.g. methods for producing a plant, vectors, plant cells, plants, biomass and wood comprising the DNA construct and vice versa. A DNA construct described in relation to a plant also regards all other embodiments.

[0206] All patent and non-patent references cited in the present application, are hereby incorporated by reference in their entirety.

[0207] The invention will now be described in further details in the following non-limiting examples.

EXAMPLES

Introduction

[0208] In order to find and elucidate the function of genes involved in growth, an extensive gene mining program was performed, resulting in the identification of genes useful in increasing growth which are of industrial application.

Materials and Methods

Gene Selection

[0209] The first step in this gene mining program was to select a number of genes from a large gene pool in order to narrow the genes to be tested for their function.

[0210] We decided to test transcription factors. The reason to select transcription factors for analysis is because they are long known to be part regulators of many if not most processes in living organisms plants.

[0211] Although the selection of the genes, for which functions are to be analysed, is an important part of the discovery of genes with functions interesting for forest biotechnology in an economic efficient way, it is the actual testing of the gene function of the selected genes which is the crucial step for finding their use in industrial applications.

[0212] The Transcription Factor genes were identified by BLAST analysis of the genes present in Populus DB, Sterky et al. 2004, against plant genes annotated as transcription factors present in databases such as http://plntfdb.bio.uni-potsdam.de/v2.0/, described in Riano-Pachon et al 2007 and http://www.ncbi.nlm.nih.gov/. In some instances were genes also selected based on having an differential expression pattern during wood formation (the genes corresponding to the constructs TFSTT 019, 035, 047 and 051).

Cloning of the Selected Genes

[0213] The corresponding gene models for the selected genes were extracted from data derived from the genome sequencing of Populus trichocarpa, Tuskan et al. 2006 using BLAST analysis. The gene models were compared to, and in some instances corrected based on, information published for homologous genes in Arabidopsis thaliana and other plant species. This was done using databases such as http://www.ncbi.nlm.nih.gov/ and http://www.arabidopsis.org/. Selected genes were subsequently cloned into an over-expression vector under the control of the CaMV 35S promoter. For isolation of cDNA, total RNA was isolated from stem, leaf and bark tissue sampled from hybrid aspen clone T89 plants and reverse transcribed to cDNA using Superscript III First Strand Synthesis System (Invitrogen). cDNA were then amplified by PCR with gene specific forward and reverse primers using Phusion high fidelity DNA polymerase (Finnzymes). PCR primers were selected as follows, the 5'-primer was placed at the start codon and the 3' reverse primer was placed 3' of the translational stop site. Forward primers were modified by the introduction of a Kozak sequence (5''-AGAACC-3'') upstream and next to the start codon of each target gene. The amplified cDNAs were inserted into a Gateway entry vector pENTR/D-TOPO (Invitrogen), followed by transfer of the genes into the expression vector pK2GW7 (SEQ ID NO:96) using the Gateway LR recombination reaction (Invitrogen). The cloned genes were control sequenced and compared to the selected genes using standard techniques before sub cloning into the plant vector pK2GW7.

[0214] The sequences of the genes, the polypeptide sequences and PCR primers for the genes presented here are listed in Table A to C.

TABLE-US-00001 TABLE A PCR cloning primers Forward Reverse cloning Construct cloning primer primer TF0002 SEQ ID NO: 27 SEQ ID NO: 40 TF0052 SEQ ID NO: 28 SEQ ID NO: 41 TF0065 SEQ ID NO: 29 SEQ ID NO: 42 TF0076 SEQ ID NO: 30 SEQ ID NO: 43 TF0089 SEQ ID NO: 31 SEQ ID NO: 44 TF0109 SEQ ID NO: 32 SEQ ID NO: 45 TF0132 SEQ ID NO: 33 SEQ ID NO: 46 TFSTT051 SEQ ID NO: 34 SEQ ID NO: 47 TF0013 SEQ ID NO: 35 SEQ ID NO: 48 TF0097 SEQ ID NO: 36 SEQ ID NO: 49 TFSTT019 SEQ ID NO: 37 SEQ ID NO: 50 TFSTT035 SEQ ID NO: 38 SEQ ID NO: 51 TFSTT047 SEQ ID NO: 39 SEQ ID NO: 52 TF0003 SEQ ID NO: 135 SEQ ID NO: 154 TF0011 SEQ ID NO: 136 SEQ ID NO: 155 TF0045 SEQ ID NO: 137 SEQ ID NO: 156 TF0058 SEQ ID NO: 138 SEQ ID NO: 157 TF0096 SEQ ID NO: 139 SEQ ID NO: 158 TF0104 SEQ ID NO: 140 SEQ ID NO: 159 TF0116 SEQ ID NO: 141 SEQ ID NO: 160 TF0146 SEQ ID NO: 142 SEQ ID NO: 161 TF0173 SEQ ID NO: 143 SEQ ID NO: 162 TF0247 SEQ ID NO: 144 SEQ ID NO: 163 TF0405 SEQ ID NO: 145 SEQ ID NO: 164 TFSTT001 SEQ ID NO: 146 SEQ ID NO: 165 TFSTT004 SEQ ID NO: 147 SEQ ID NO: 166 TFSTT013 SEQ ID NO: 148 SEQ ID NO: 167 TFSTT016 SEQ ID NO: 149 SEQ ID NO: 168 TFSTT017 SEQ ID NO: 150 SEQ ID NO: 169 TFSTT036 SEQ ID NO: 151 SEQ ID NO: 170 TFSTT038 SEQ ID NO: 152 SEQ ID NO: 171 TFSTT045 SEQ ID NO: 153 SEQ ID NO: 172 Binary destination vector: SEQ ID NO: 96 pK2GW7

TABLE-US-00002 TABLE B Over-expressed gene cDNA and polypeptide sequences Gene model Predicted for the sequence over-expressed of over- gene cDNA expressed Construct sequence protein Species TF0002 SEQ ID NO: 1 SEQ ID NO: 14 Populus trichocarpa TF0052 SEQ ID NO: 2 SEQ ID NO: 15 Populus trichocarpa TF0065 SEQ ID NO: 3 SEQ ID NO: 16 Populus trichocarpa TF0076 SEQ ID NO: 4 SEQ ID NO: 17 Populus trichocarpa TF0089 SEQ ID NO: 5 SEQ ID NO: 18 Populus trichocarpa TF0109 SEQ ID NO: 6 SEQ ID NO: 19 Populus trichocarpa TF0132 SEQ ID NO: 7 SEQ ID NO: 20 Populus trichocarpa TFSTT051 SEQ ID NO: 8 SEQ ID NO: 21 Populus trichocarpa TF0013 SEQ ID NO: 9 SEQ ID NO: 22 Populus trichocarpa TF0097 SEQ ID NO: 10 SEQ ID NO: 23 Populus trichocarpa TFSTT019 SEQ ID NO: 11 SEQ ID NO: 24 Populus trichocarpa TFSTT035 SEQ ID NO: 12 SEQ ID NO: 25 Populus trichocarpa TFSTT047 SEQ ID NO: 13 SEQ ID NO: 26 Populus trichocarpa TF0003 SEQ ID NO: 97 SEQ ID NO: 116 Populus trichocarpa TF0011 SEQ ID NO: 98 SEQ ID NO: 117 Populus trichocarpa TF0045 SEQ ID NO: 99 SEQ ID NO: 118 Populus trichocarpa TF0058 SEQ ID NO: 100 SEQ ID NO: 119 Populus trichocarpa TF0096 SEQ ID NO: 101 SEQ ID NO: 120 Populus trichocarpa TF0104 SEQ ID NO: 102 SEQ ID NO: 121 Populus trichocarpa TF0116 SEQ ID NO: 103 SEQ ID NO: 122 Populus trichocarpa TF0146 SEQ ID NO: 104 SEQ ID NO: 123 Populus trichocarpa TF0173 SEQ ID NO: 105 SEQ ID NO: 124 Populus trichocarpa TF0247 SEQ ID NO: 106 SEQ ID NO: 125 Populus trichocarpa TF0405 SEQ ID NO: 107 SEQ ID NO: 126 Populus trichocarpa TFSTT001 SEQ ID NO: 108 SEQ ID NO: 127 Populus trichocarpa TFSTT004 SEQ ID NO: 109 SEQ ID NO: 128 Populus trichocarpa TFSTT013 SEQ ID NO: 110 SEQ ID NO: 129 Populus trichocarpa TFSTT016 SEQ ID NO: 111 SEQ ID NO: 130 Populus trichocarpa TFSTT017 SEQ ID NO: 112 SEQ ID NO: 131 Populus trichocarpa TFSTT036 SEQ ID NO: 113 SEQ ID NO: 132 Populus trichocarpa TFSTT038 SEQ ID NO: 114 SEQ ID NO: 133 Populus trichocarpa TFSTT045 SEQ ID NO: 115 SEQ ID NO: 134 Populus trichocarpa

TABLE-US-00003 TABLE C Control sequences of cloned cDNA Full control 5' control 3' control sequence of sequence of sequence of Construct cloned cDNA cloned cDNA cloned cDNA Species TF0002 SEQ ID NO: 73 Populus tremula x tremuloides TF0052 SEQ ID NO: 74 Populus tremula x tremuloides TF0065 SEQ ID NO: 76 SEQ ID NO: 86 Populus tremula x tremuloides TF0076 SEQ ID NO: 77 SEQ ID NO: 87 Populus tremula x tremuloides TF0089 SEQ ID NO: 78 SEQ ID NO: 88 Populus tremula x tremuloides TF0109 SEQ ID NO: 75 Populus tremula x tremuloides TF0132 SEQ ID NO: 79 SEQ ID NO: 89 Populus tremula x tremuloides TFSTT051 SEQ ID NO: 80 SEQ ID NO: 90 Populus tremula x tremuloides TF0013 SEQ ID NO: 81 SEQ ID NO: 91 Populus tremula x tremuloides TF0097 SEQ ID NO: 82 SEQ ID NO: 92 Populus tremula x tremuloides TFSTT019 SEQ ID NO: 83 SEQ ID NO: 93 Populus tremula x tremuloides TFSTT035 SEQ ID NO: 84 SEQ ID NO: 94 Populus tremula x tremuloides TFSTT047 SEQ ID NO: 85 SEQ ID NO: 95 Populus tremula x tremuloides TF0003 SEQ ID NO: 173 Populus tremula x tremuloides TF0011 SEQ ID NO: 174 Populus tremula x tremuloides TF0045 SEQ ID NO: 180 SEQ ID NO: 192 Populus tremula x tremuloides TF0058 SEQ ID NO: 175 Populus tremula x tremuloides TF0096 SEQ ID NO: 181 SEQ ID NO: 193 Populus tremula x tremuloides TF0104 SEQ ID NO: 182 SEQ ID NO: 194 Populus tremula x tremuloides TF0116 SEQ ID NO: 183 SEQ ID NO: 195 Populus tremula x tremuloides TF0146 SEQ ID NO: 184 SEQ ID NO: 196 Populus tremula x tremuloides TF0173 SEQ ID NO: 176 Populus tremula x tremuloides TF0247 SEQ ID NO: 177 Populus tremula x tremuloides TF0405 SEQ ID NO: 185 SEQ ID NO: 197 Populus tremula x tremuloides TFSTT001 SEQ ID NO: 186 SEQ ID NO: 198 Populus tremula x tremuloides TFSTT004 SEQ ID NO: 187 SEQ ID NO: 199 Populus tremula x tremuloides TFSTT013 SEQ ID NO: 178 Populus tremula x tremuloides TFSTT016 SEQ ID NO: 188 SEQ ID NO: 200 Populus tremula x tremuloides TFSTT017 SEQ ID NO: 189 SEQ ID NO: 201 Populus tremula x tremuloides TFSTT036 SEQ ID NO: 190 SEQ ID NO: 202 Populus tremula x tremuloides TFSTT038 SEQ ID NO: 191 SEQ ID NO: 203 Populus tremula x tremuloides TFSTT045 SEQ ID NO: 179 Populus tremula x tremuloides

Plant Transformation

[0215] CaMV 35S: over-expression DNA constructs were transformed into Agrobacterium and subsequently into Hybrid aspen, where Populus tremula L.×P. tremuloides Minch clone T89, hereafter called "poplar", was transformed and regenerated essentially as described in Nilsson et al. (1992). Approximately 3-8 independent lines were generated for each construct. One such group of transgenic trees produced using one construct is hereafter called a "construction group", e.g. different transgenic trees emanating from one construct. Each transgenic line within each construction group, e.g. TF0555-2B, TF0555-3A, and so on, are different transformation events and therefore most probably have the recombinant DNA inserted into different locations in the plant genome. This makes the different lines within one construction group partly different. For example it is known that different transformation events will produce plants with different levels of gene over-expression. Construction groups named for example TF0555RP with individuals such as TF055RP-2B, are the same as the one without the RP part. RP means that this is a re-planting of the same construction group as the one without the rp part. RP2 means the second re-planting, RP3 the third re-planting and so on.

Plant Growth

[0216] The transgenic poplar lines were grown together with their wildtype control (wt) trees, in a greenhouse under a photoperiod of 18 h and a temperature of 22° C./15° C. (day/night). The plants were fertilized weekly with Weibulls Rika S NPK 7-1-5 diluted 1 to 100 (final concentrations NO₃, 55 g/l; NH₄, 29 g/l; P, 12 g/l; K, 56 g/l; Mg 7.2 g/l; S, 7.2 g/l; B, 0.18 g/l; Cu, 0.02 g/l; Fe, 0.84 g/l; Mn, 0.42 g/l; Mo, 0.03 g/l; Zn, 0.13 g/L). The plants were grown for 8-9 weeks before harvest. During this time their height and diameter was measured one to two times per week. In a growth group a number of wildtype trees (typically 35-45 trees) and a number of transgenic trees comprising several construction groups (typically 6-20 construction groups) were grown in parallel in the greenhouse under the same above conditions. All comparisons between the wildtype trees and construction groups are made within each growth group.

Sampling

[0217] Two principal types of harvests and samplings were performed. One general type was designed for example chemical analysis, wood morphology analysis, gene expression analysis, wood density analysis and metabolomics analysis. The second type was designed for dry weight measurements of bark, wood, leafs and roots.

Selection of Construction Groups

[0218] In the first round of growth for each group of trees with a specific gene over-expressed, i.e. a construction group, a number of the following analyses were performed: Growth measurements and in many cases wood density. These data were analysed in order to single out the construction groups that showed a phenotypic variation, e.g. increased growth compared to wild type control trees.

Replant and Regrowth

[0219] Based on growth data in the first round of greenhouse growth, groups of trees, with a specific gene over-expressed, i.e. a construction group, were selected, replanted and regrown under the same conditions as in the first round of growth. Selected transgenic poplar lines within each construction group were regrown in triplicates. Replant round number and plant line individual replicate numbers were added to the names of the construction group lines to keep them unique, e.g. TF0555rp1-2B-1, TF0555rp1-2B-2, TF0555rp1-2B-3, where rp1 means first round of replanting of construction group TF0555 line 2B and -1, -2, -3 denotes plant line individual replicates. Similarly rp2 means second round of replanting. In cases where new construction group lines, not included in the first round of greenhouse growth, are planted, a suffix (0.2nd) is added to construction group name to illustrate this.

[0220] Based on growth data a number of analyses and growth rate factors were performed and calculated in order to select the construction groups and thereby the genes which are possible to use for altering growth characteristics. Selection criteria and methods were as described below.

Example 1

Growth Analysis

Maximum Height Growth Rate

[0221] A height growth rate measure (here named "Maximum height growth rate") was defined as the slope of a linear function fitted over four consecutive height data points. A height growth rate value was calculated for data point 1-4, data point 2-5 etc. in a step-wise manner, see FIG. 1 for an example. A maximum height growth rate defined as the maximum value produced from step-wise linear regression analysis for each plant was computed. The primary data for high Maximum height growth rate values from individual transformants in a construction group were checked so they were not based on bad values. From FIG. 1, showing an example of a height growth curve, it can be seen that the height growth rate increases during the first part of growth then the plants reach their maximum height growth rate and then the growth rate declines as the plants become larger. Because these phases have different timing in different plants and there are some noise added measuring the plants our above described Maximum height growth using rate method is very useful in calculating the maximum growth speed in these conditions for the different individual trees.

Diameter Growth Rate

[0222] Under the above defined growth conditions, stem width exhibit a comparatively linear increase over time described by the formula d(t)=c*t+d₀ where d₀ is the initial width and c is the rate of diameter growth (slope). Linear regression on diameter data was used for estimating diameter growth rate.

Final Height and Diameter

[0223] The final heights and diameters were also used to select construction groups with altered growth characteristics. These values take into account both the trees growth capacity and the trees ability to start growing when transferred from tissue culture into soil and placed in a greenhouse.

Selection Parameters

[0224] Construction groups that showed a significant or pronounced increase compared to the wild type population in the above mentioned growth parameters, i.e. diameter growth rate, maximum height growth rate, final height and final diameter, were identified as construction groups that have altered growth properties. Therefore, the corresponding genes can be used to alter these properties. The selection criteria's are stated below. Two different selection criteria levels were used, one basic level and one for constructs giving growth phenotypes of extra interest.

Growth Difference Selection Criteria

[0225] Table 1.2 lists the abbreviations used for the different growth parameters when used to describe construction group phenotypes.

TABLE-US-00004 TABLE 1.2 Abbreviations used for the different phenotypes AFH Average final height of the wild type population and each construction group population AFD Average final diameter of the wild type population and each construction group population AMHGR Average Maximum height growth rate of the wild type population and each construction group population ADGR Average diameter growth rate of the wild type population and each construction group population MFH Maximum final height of the wild type population and each construction group population MFD Maximum final diameter of the wild type population and each construction group population MMHGR Maximum of Maximum height growth rate of the wild type population and each construction group population MDC Maximum diameter growth rate of the wild type population and each construction group population

[0226] The growth difference selection criteria are as follows:

[0227] 1. If construction group AFH, MFH, AMHGR and MMHGR are at least 5% (or 8% in a second more stringent level) greater than corresponding wild type group AFH, MFH, AMHGR and MMHGR, or

[0228] 2. If construction group AFD, MFD, ADGR and MDC are at least 5% (or 8% in a second more stringent level) greater than corresponding wild type group AFD, MFD, ADGR and MDC, or

[0229] 3. If construction group AFH, AFD, AMHGR or ADGR is at least 18% (or 22% in the second more stringent level) greater than corresponding wild type group AFH, AFD, AMHGR or ADGR, or

[0230] 4. If construction group MFH, MFD, MMHGR or MDC is at least 18% (or 22% in the second more stringent level) greater than corresponding wild type group MFH, MFD, MMHGR or MDC

[0231] Running a large scale functional genomics program produces a certain amount of variation and uncertainty in the data produced. In this setup variation is originating from sources such as: the different lines within an construction group have different levels of over-expression resulting in that one to all tested lines within an construction group can show the phenotype; the variation in growth that occur during the experimental procedure due to small variations in plant status when transferring the plants from tissue culture to the greenhouse and variations based on different positions in the greenhouse during different time points during the growth cycle. These variations have to be dealt with when analysing the data. Based on this we used two different thresholds of increase 5% and 18% for selecting construction groups with increased growth. The selection criteria 1 and 2 uses an 5% increase, however this increase have to be present in all the phenotypes AFH, MFH, AMHGR and MMHGR corresponding to height growth or all the phenotypes AFD, MFD, ADGR and MDC corresponding to diameter growth. In the cases that the phenotype only can be seen in some or one of the plants and only in one phenotype class, an higher 18% increase were used to select positive construction groups in order not to select construction groups based on random variations (selection criteria's 3 and 4 selecting on average values and maximum individual values respectively).

[0232] Construction groups meeting one or more of these criteria were selected.

Over-Expression Level Analysis

[0233] Real-time RT PCR was used to compare construct gene expression levels of the recombinant over-expression construction group with corresponding wild type group. The expression level of 26S proteasome regulatory subunit S2 was used as a reference to which construct gene expression was normalized. The comparative CT method was used for calculation of relative construct gene expression levels, where the ratio between construction and reference gene expression levels is described by (1+E_target)^-CTtarget/(1+E_reference)^-CTreference where E_target and E_reference are the efficiencies of construct and reference gene PCR amplification respectively and CT_target and CT_reference are the threshold cycles as calculated for construct and reference gene amplification respectively. The ratios between construct and reference gene expression levels were subsequently normalized to the average of wild type group ratios.

[0234] For total RNA extraction, stem samples (approx. 50 mg) were harvested from greenhouse grown plants and flash frozen in liquid nitrogen. Frozen samples were ground in a bead mill (Retsch MM301). Total RNA was extracted using E-Z 96 Plant RNA kit according to manufacturer's recommendations (Omega Bio-Tek). cDNA synthesis was performed using iScript cDNA synthesis kit according to manufacturer's recommendations (Bio-Rad). RNA concentrations were measured and equal amounts were used for cDNA synthesis to ensure equal amounts of cDNA for PCR reactions. The cDNA was diluted 12.5× prior to real-time PCR.

[0235] Real-time PCR primers were designed using Beacon Designer 6 (PREMIER Biosoft International) using included tool to minimize interference of template secondary structure at primer annealing sites.

[0236] For real-time PCR, cDNA template was mixed with corresponding construct gene specific primers (SEQ ID NO: 53-61 and SEQ ID NO: 63-71), internal reference gene specific primers (SEQ ID NO: 62 and 72) and SYBR Green Supermix (Bio-Rad). Real-time PCR reactions were run on a MyiQ PCR thermocycler (Bio-Rad) and analysed using included software iQ5. Reactions were set up in triplicates, three times using construct gene specific primers and three times using reference gene specific primers for each sample, and the average threshold cycle for each triplicate was subsequently used for calculation of relative construct gene expression levels.

[0237] The 96 well plate was covered with microfilm and set in the thermocycler to start the reaction cycle. By way of illustration, the reaction cycle may include the following steps: Initial denaturation at 95° C. for 3 minutes 30 seconds followed by 40 rounds of amplification comprising the following steps 95° C. for 10 seconds, 55° C. for 30 seconds and 72° C. for 40 seconds.

TABLE-US-00005 TABLE 1.3 Real-time RT-PCR primers Forward real-time Reverse real-time Construct RT-PCR primer RT-PCR primer TF0002 SEQ ID NO: 53 SEQ ID NO: 63 TF0052 SEQ ID NO: 54 SEQ ID NO: 64 TF0065 SEQ ID NO: 55 SEQ ID NO: 65 TF0076 SEQ ID NO: 56 SEQ ID NO: 66 TF0089 SEQ ID NO: 57 SEQ ID NO: 67 TF0109 SEQ ID NO: 58 SEQ ID NO: 68 TFSTT051 SEQ ID NO: 59 SEQ ID NO: 69 TF0013 SEQ ID NO: 60 SEQ ID NO: 70 TF0097 SEQ ID NO: 61 SEQ ID NO: 71 Real-time RT PCR reference gene: SEQ ID NO: 62 SEQ ID NO: 72 26S proteasome regulatory subunit S2

Results

[0238] Growth raw data for the specified construction group and the corresponding wild type group are shown in tables 1.4 to 1.16. Table rows contain height and diameter measurements of individuals of specified construction group (named "TF") and corresponding wild type group (named "T89"). Time of measurement as number of days in greenhouse is shown in table headers.

[0239] Real-time RT-PCR was used to confirm over-expression of constructs. Real-time RT-PCR data tables contain gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios.

Construction Group TF0002

[0240] This construct induces increased growth. The final height is 12% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 31% higher comparing the average of the construction group and wild type control group. The TF0002 construction group meets the more stringent level of growth difference selection criterion (3) as shown in table 1.4d.

[0241] Tables 1.4a and 1.4b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00006 TABLE 1.4a Height growth data for TF0002 Height (cm) Days in greenhouse 18 22 25 29 32 39 46 50 53 TF0002-1B 12 18 23 35 49 82 115 134 150 TF0002-2A 15 21 27 40 55 87 122 142 157 TF0002-2B 9 12 15 23 36 64 95 113 129 TF0002-3A 17 22 29 42 57 92 129 150 166 TF0002-3B 16 21 24 39 52 83 117 136 154 TF0002-4B 14 19 25 38 52 85 121 141 159 T89-01 25 29 34 45 54 79 104 119 132 T89-02 28 33 41 54 64 83 109 125 136 T89-03 26 34 41 51 61 90 125 143 155 T89-04 29 36 42 58 72 98 127 146 159 T89-05 21 25 29 37 45 68 93 108 120 T89-06 25 31 39 49 60 83 109 125 137 T89-07 24 29 34 45 58 83 109 125 138 T89-08 24 32 41 53 67 94 121 138 150 T89-09 24 32 41 54 66 94 120 135 145 T89-10 20 26 30 44 54 79 108 123 135 T89-11 21 26 32 42 54 79 107 123 136 T89-12 25 32 40 55 66 97 125 140 151 T89-13 21 29 35 45 54 75 98 114 124 T89-14 25 31 38 50 60 85 111 125 135 T89-15 25 33 40 53 64 88 112 126 137 T89-16 24 28 33 42 49 68 89 102 112 T89-17 26 33 40 50 60 83 109 127 137 T89-18 24 29 37 47 55 80 108 123 133 T89-19 21 26 32 40 49 65 80 95 107 T89-20 26 32 40 52 65 98 127 146 159 T89-21 24 29 37 45 51 71 100 114 125 T89-22 25 30 39 48 63 83 110 125 139 T89-23 22 28 34 45 56 83 106 120 130 T89-24 24 30 38 49 60 82 106 124 138 T89-25 24 29 36 48 56 80 107 123 132 T89-26 26 35 41 52 68 87 110 123 136 T89-27 22 28 35 47 57 84 111 126 138 T89-28 24 29 36 46 58 87 110 123 136 T89-29 24 32 40 50 63 81 111 128 141 T89-30 26 31 37 46 53 72 95 107 118 T89-31 26 32 41 52 63 87 115 130 143 T89-32 28 33 41 52 63 88 116 130 142 T89-33 20 24 30 41 51 76 102 117 130 T89-34 25 30 39 51 63 90 117 130 138 T89-35 26 31 38 49 59 83 106 119 129 T89-36 25 33 40 52 59 73 91 104 116 T89-37 29 37 45 60 73 100 128 146 156

TABLE-US-00007 TABLE 1.4b Diameter growth data for TF0002 Diameter (mm) Days in greenhouse 29 32 39 46 50 53 TF0002-1B 3.4 4.7 6.0 7.3 7.5 8.1 TF0002-2A 3.5 4.1 5.7 7.0 7.4 7.3 TF0002-2B 2.9 3.3 4.3 5.7 6.2 7.0 TF0002-3A 3.8 4.8 6.2 7.4 8.3 8.8 TF0002-3B 3.5 4.4 5.7 6.7 7.4 8.1 TF0002-4B 3.4 4.8 5.8 7.1 7.5 8.0 T89-01 3.8 4.6 5.9 7.2 7.6 8.1 T89-02 5.0 5.9 7.0 8.4 9.5 8.9 T89-03 4.9 6.4 7.3 8.8 8.8 9.6 T89-04 5.1 6.2 8.1 9.0 8.6 10.2 T89-05 3.8 4.2 5.0 6.1 5.9 6.7 T89-06 5.0 5.9 7.0 7.9 8.9 9.1 T89-07 4.0 5.0 6.4 7.5 8.3 9.3 T89-08 4.6 5.6 7.1 8.1 8.6 9.9 T89-09 5.1 6.2 8.0 9.3 9.7 10.3 T89-10 3.7 5.0 6.2 7.3 8.3 8.7 T89-11 3.8 4.5 6.0 7.4 8.2 8.7 T89-12 4.6 6.3 7.1 8.6 9.4 10.8 T89-13 5.0 5.2 6.0 6.8 7.2 7.7 T89-14 4.3 5.1 6.5 7.3 7.7 8.5 T89-15 5.6 6.0 7.4 8.4 8.9 9.6 T89-16 4.1 5.0 6.1 6.9 7.0 7.8 T89-17 5.5 6.2 7.2 7.9 8.7 9.0 T89-18 4.6 4.9 6.0 7.2 7.9 8.7 T89-19 4.0 5.2 6.5 7.7 7.0 8.4 T89-20 4.6 6.0 7.5 9.8 10.3 8.8 T89-21 4.5 4.7 5.6 6.8 5.8 7.8 T89-22 4.7 5.4 6.1 6.9 6.9 8.1 T89-23 4.0 5.0 6.2 7.7 8.5 9.0 T89-24 4.4 5.0 5.5 6.8 6.9 7.9 T89-25 4.4 5.5 6.1 7.8 8.2 7.3 T89-26 4.4 4.8 6.2 7.6 6.3 8.5 T89-27 4.4 5.1 5.8 7.1 9.0 7.2 T89-28 4.0 5.0 6.3 7.4 8.1 8.9 T89-29 4.7 6.0 7.1 8.3 7.8 9.3 T89-30 4.3 4.8 5.5 6.0 6.6 7.1 T89-31 4.6 5.5 6.4 7.8 9.0 9.3 T89-32 4.5 5.2 6.1 7.0 7.9 8.7 T89-33 3.9 4.4 5.6 6.5 5.7 7.7 T89-34 4.5 5.7 6.6 8.3 9.0 9.5 T89-35 4.2 5.4 7.1 8.5 9.3 10.0 T89-36 5.0 5.6 7.0 8.3 7.1 8.9 T89-37 5.1 6.0 6.9 8.1 9.1 9.6

[0242] Real-time RT-PCR was used to confirm over-expression of construct TF0002. Table 1.4c contains gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios. All individuals of construction group TF0002 are over-expressed according to present RT-PCR data.

TABLE-US-00008 TABLE 1.4c Real-time RT-PCR data for TF0002 Relative gene expression level Sample normalized to wild type average TF0002-1B 8.63 TF0002-2A 7.88 TF0002-2B 8.18 TF0002-3A 5.88 TF0002-3B 5.72 TF0002-4B 8.89 T89-06 1.09 T89-26 1.47 T89-29 0.88 T89-31 0.84 T89-32 0.71

[0243] Results from growth analysis are specified in the overview table 1.4d. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00009 TABLE 1.4d Overview table of growth effects of construct TF0002 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0002 1.12 0.90 1.31 1.06 1.04 0.81 1.13 0.85

Construction Group TF0052

[0244] This construct induces increased growth. The final height is 24% higher comparing the largest individuals of the construction group and wild type control group. The TF0052 construction group meets the more stringent level of growth difference selection criterion (4) as shown in table 1.5c.

[0245] Tables 1.5a and 1.5b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00010 TABLE 1.5a Height growth data for TF0052 Height (cm) Days in greenhouse 19 28 34 40 44 47 51 54 61 65 TF0052-1A 8 17 26 35 39 42 48 51 58 64 TF0052-1B 18 32 50 65 79 89 99 107 125 135 TF0052-2A 18 40 64 83 97 108 122 131 156 172 TF0052-2B 13 29 47 59 72 80 90 99 120 132 TF0052-3A 18 36 58 71 84 91 102 107 119 124 TF0052-3B 15 33 54 69 82 90 100 107 124 135 TF0052-4A 14 27 46 58 68 76 85 93 110 122 TF0052-4B 19 36 55 68 79 87 96 98 113 121 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

TABLE-US-00011 TABLE 1.5b Diameter growth data for TF0052 Diameter (mm) Days in greenhouse 28 34 40 44 47 51 54 61 65 TF0052-1A 3.5 4.1 4.9 4.6 5.2 5.0 5.6 6.7 6.2 TF0052-1B 3.1 4.4 6.0 6.6 6.9 8.0 8.2 9.3 9.4 TF0052-2A 4.5 6.3 8.1 8.6 9.7 9.8 10.2 11.5 11.5 TF0052-2B 3.5 5.2 6.1 7.5 7.8 8.8 9.5 10.9 11.0 TF0052-3A 3.5 5.4 6.7 7.2 7.8 7.9 8.1 8.7 9.3 TF0052-3B 3.8 5.5 7.1 7.2 8.2 8.4 9.0 9.1 9.6 TF0052-4A 3.4 4.9 6.5 6.7 7.0 7.8 8.0 9.1 9.3 TF0052-4B 3.5 4.9 6.0 7.0 7.2 7.6 7.8 8.1 8.7 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 N/A 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[0246] Results from growth analysis are specified in the overview table 1.5c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00012 TABLE 1.5c Overview table of growth effects of construct TF0052 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0052 1.00 0.98 0.95 0.89 1.24 0.93 1.08 0.91

Construction Group TF0065

[0247] This construct induces increased growth. The final height is 8% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 11% higher comparing the average of the construction group and wild type control group. The TF0065 construction group meets growth difference selection criterion (1) as shown in table 1.6c.

[0248] Tables 1.6a and 1.6b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00013 TABLE 1.6a Height growth data for TF0065 Days in Height (cm) greenhouse 18 22 25 29 32 39 43 46 50 53 57 TF0065-1AA 20 26 33 46 58 88 101 111 123 134 151 TF0065-1AB 23 27 34 48 60 89 106 115 133 146 164 TF0065-1BA 21 27 33 45 56 82 97 107 122 135 153 TF0065-1BB 22 26 32 44 56 84 101 113 130 144 164 TF0065-2B 24 29 37 51 64 96 115 127 145 162 181 TF0065-3A 20 28 33 43 54 79 94 106 124 138 155 TF0065-4B 16 20 28 40 49 73 85 94 108 121 138 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

TABLE-US-00014 TABLE 1.6b Diameter growth data for TF0065 Days in Diameter (mm) greenhouse 29 32 39 43 46 50 53 57 TF0065-1AA 3.9 5.0 6.2 6.7 6.9 7.3 8.5 8.3 TF0065-1AB 4.0 5.1 6.9 8.4 8.0 9.5 9.5 10.6 TF0065-1BA 4.3 4.9 5.9 6.3 6.8 7.5 7.8 8.4 TF0065-1BB 3.8 4.8 6.0 7.1 7.4 8.4 9.3 10.1 TF0065-2B 5.9 4.8 7.1 8.0 8.6 9.5 9.7 10.2 TF0065-3A 4.2 4.9 6.0 6.9 7.1 7.6 8.4 9.2 TF0065-4B 3.3 4.0 4.8 5.5 5.8 N/A 6.9 7.9 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 N/A 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7.0 7.5 8.6 9.4 9.8

[0249] Results from growth analysis are specified in the overview table 1.6c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00015 TABLE 1.6c Overview table of growth effects of construct TF0065 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0065 1.08 0.99 1.11 0.96 1.07 1.01 1.06 0.97

Construction Group TF0076

[0250] This construct induces increased growth. The final height is 10% higher comparing the average of the construction group and wild type control group. The final height is 18% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 13% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 18% higher comparing the largest individuals of the construction group and wild type control group. The TF0076 construction group meets the more stringent level of growth difference selection criterion (1) and the less stringent level of growth difference selection criterion (4) as shown in table 1.7d.

[0251] Tables 1.7a and 1.7b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00016 TABLE 1.7a Height growth data for TF0076 Days in Height (cm) greenhouse 18 22 25 29 32 39 43 46 50 53 57 TF0076-2AA 18 24 30 42 52 76 92 103 118 133 150 TF0076-2AB 18 25 33 44 55 81 94 104 117 128 143 TF0076-3BA 16 20 26 36 47 70 86 97 112 126 142 TF0076-3BB 23 28 35 49 61 90 104 115 130 143 155 TF0076-4B N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A TF0076-5BA 20 25 33 45 55 87 106 121 140 157 177 TF0076-5BB 24 32 40 57 71 105 127 140 160 180 200 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

TABLE-US-00017 TABLE 1.7b Diameter growth data for TF0076 Days in Diameter (mm) greenhouse 29 32 39 43 46 50 53 57 TF0076-2AA 3.8 4.5 6.6 7.5 8.0 9.1 9.9 10.4 TF0076-2AB 4.8 5.0 5.6 6.3 6.8 7.3 7.9 8.1 TF0076-3BA 3.1 3.8 5.5 6.6 7.5 7.6 8.8 8.9 TF0076-3BB 3.8 4.7 6.2 7.4 8.5 8.7 9.4 10.0 TF0076-4B N/A N/A N/A N/A N/A N/A N/A N/A TF0076-5BA 3.7 4.9 6.1 7.0 7.8 9.1 9.7 9.8 TF0076-5BB 4.2 6.0 7.2 8.9 8.9 9.8 10.1 10.6 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 N/A 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7.0 7.5 8.6 9.4 9.8

[0252] Real-time RT-PCR was used to confirm over-expression of construct TF0076. Table 1.7c contains gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios. 4 of 6 individuals of construction group TF0076 are over-expressed according to present RT-PCR data.

TABLE-US-00018 TABLE 1.7c Real-time RT-PCR data for TF0076 Relative gene expression level Sample normalized to wild type average TF0076-2AA 0.43 TF0076-2AB 3.25 TF0076-3BA 3.61 TF0076-3BB 0.65 TF0076-5BA 3.70 TF0076-5BB 3.63 T89-03 1.46 T89-36 1.54 T89-37 0.52 T89-38 0.66 T89-39 0.82

[0253] Results from growth analysis are specified in the overview table 1.7d. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00019 TABLE 1.7d Overview table of growth effects of construct TF0076 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0076 1.10 1.04 1.13 1.09 1.18 1.01 1.18 1.02

Construction Group TF0089

[0254] This construct induces increased growth. The final height is 7% higher comparing the average of the construction group and wild type control group. The final height is 17% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 12% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 17% higher comparing the largest individuals of the construction group and wild type control group. The TF0089 construction group meets growth difference selection criterion (1) as shown in table 1.8c.

[0255] Tables 1.8a and 1.8b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00020 TABLE 1.8a Height growth data for TF0089 Days in Height (cm) greenhouse 18 21 26 32 35 39 43 46 53 60 64 TF0089-1A 4 7 13 25 35 48 60 71 94 122 140 TF0089-1BA 19 22 31 51 61 71 84 92 115 138 152 TF0089-1BB 10 13 19 34 41 52 67 78 103 125 138 TF0089-2AA 23 29 37 52 61 71 86 97 119 144 158 TF0089-2AB 20 25 33 47 56 68 80 90 113 137 148 TF0089-2B 24 30 32 60 70 80 98 111 140 168 184 T89-01 20 27 35 54 61 72 84 93 112 130 141 T89-02 19 25 31 48 56 67 79 90 113 137 149 T89-03 21 26 33 48 55 64 75 83 100 117 128 T89-04 21 26 33 52 59 69 83 96 120 140 152 T89-05 20 25 34 52 59 70 84 120 144 156 T89-06 20 26 35 52 61 73 87 98 121 143 156 T89-07 17 21 27 45 53 63 74 85 107 129 140 T89-08 18 24 31 48 56 64 75 86 106 128 141 T89-09 24 28 34 48 55 65 78 91 112 136 153 T89-10 18 24 30 44 52 62 75 87 107 129 142 T89-11 11 14 20 32 41 52 62 72 95 118 132 T89-12 17 24 30 44 52 62 74 85 106 129 141 T89-13 21 28 35 48 56 67 78 85 103 122 134 T89-14 20 25 34 51 60 69 80 90 110 135 148 T89-15 19 24 32 45 52 62 74 84 106 129 142 T89-16 20 24 30 45 53 63 75 86 108 131 141 T89-17 18 23 28 42 50 61 74 82 104 129 143 T89-18 17 22 28 44 53 62 75 85 108 130 144 T89-19 19 24 30 43 50 59 72 84 103 125 140 T89-20 18 23 30 43 50 60 71 80 102 123 136 T89-21 19 24 31 45 53 62 76 87 107 134 149 T89-22 15 19 23 30 39 47 58 66 82 99 109 T89-23 19 24 34 50 60 69 83 94 118 141 154 T89-24 23 27 36 51 61 72 85 95 120 145 157 T89-25 19 21 29 44 52 63 74 84 102 124 138 T89-26 20 25 32 45 53 63 75 85 108 128 139 T89-27 22 25 33 48 56 66 80 92 112 134 147 T89-28 21 28 33 47 57 68 80 89 111 137 148 T89-29 18 23 28 43 51 61 73 84 105 125 136 T89-30 19 20 25 38 45 53 63 71 91 114 125 T89-31 15 21 29 48 57 66 80 91 114 134 148 T89-32 20 24 33 50 55 65 77 88 110 134 146 T89-33 20 26 33 49 57 68 79 89 113 135 149 T89-34 19 25 33 51 58 70 84 98 120 140 154 T89-35 19 24 31 46 54 64 78 90 112 135 147 T89-36 21 25 33 49 57 68 80 89 109 130 140 T89-37 17 23 32 49 58 68 78 88 110 133 146 T89-38 18 24 31 46 54 65 79 91 112 138 152 T89-39 20 25 32 50 59 69 82 92 118 138 151 T89-40 22 27 35 49 56 66 77 88 109 131 145 T89-41 20 24 30 44 51 60 73 82 106 129 141 T89-42 21 26 32 50 58 68 82 92 115 134 148 T89-43 17 24 32 47 55 65 79 89 113 139 154 T89-44 18 24 31 47 56 65 78 89 112 135 152 T89-45 20 23 29 45 55 63 75 86 106 129 141 T89-46 21 24 32 45 55 67 78 85 103 122 138 T89-47 19 24 30 46 53 67 74 84 105 131 144

TABLE-US-00021 TABLE 1.8b Diameter growth data for TF0089 Days in Diameter (mm) greenhouse 32 35 39 43 46 53 60 64 TF0089-1A 3.0 3.3 4.2 5.1 6.0 7.5 8.3 8.2 TF0089-1BA 4.9 6.0 6.5 7.8 8.1 9.1 9.5 10.1 TF0089-1BB 4.0 4.2 5.3 6.1 6.8 7.6 8.7 9.2 TF0089-2AA 5.7 6.3 7.3 8.6 8.1 9.6 10.9 11.5 TF0089-2AB 4.8 6.0 6.5 7.5 8.1 8.8 10.4 10.4 TF0089-2B 5.3 6.0 6.8 8.6 9.4 10.0 12.4 12.2 T89-01 5.1 5.8 6.6 7.4 8.5 8.3 9.5 9.7 T89-02 5.1 5.8 6.7 7.5 8.6 9.5 10.9 11.4 T89-03 4.6 5.3 5.8 6.4 7.0 7.5 8.2 8.7 T89-04 5.4 6.2 7.3 8.1 8.6 9.8 10.8 10.9 T89-05 5.1 6.0 7.1 7.7 8.7 9.3 10.7 11.0 T89-06 5.6 5.6 6.8 7.4 8.6 10.3 10.8 11.3 T89-07 4.1 4.8 5.6 6.7 7.3 8.5 9.8 9.9 T89-08 4.8 5.5 6.2 7.5 7.4 8.2 9.0 9.0 T89-09 5.1 5.5 6.5 7.6 7.9 9.7 10.6 10.8 T89-10 5.8 5.8 6.5 7.2 7.5 9.3 10.2 10.9 T89-11 3.8 4.4 5.5 6.1 6.7 8.6 9.9 10.1 T89-12 4.8 5.8 6.2 7.0 6.9 10.0 9.3 9.7 T89-13 5.9 5.8 7.3 8.7 9.0 10.7 11.2 11.6 T89-14 5.4 6.3 6.7 8.5 8.6 10.0 10.8 11.3 T89-15 5.0 5.5 6.6 7.3 8.1 9.7 10.3 10.3 T89-16 4.8 5.3 5.9 6.8 7.6 8.1 9.8 10.0 T89-17 4.0 4.7 6.1 6.6 6.8 9.0 9.6 10.5 T89-18 4.7 5.7 6.5 7.2 7.8 8.8 9.0 9.8 T89-19 4.5 5.4 6.1 6.8 7.2 8.4 9.8 10.1 T89-20 5.4 5.9 7.3 7.9 8.6 9.8 11.2 11.6 T89-21 5.0 5.8 5.7 8.2 8.0 9.3 10.1 11.2 T89-22 3.1 3.6 3.8 4.2 4.1 5.0 5.3 5.5 T89-23 5.2 5.8 6.7 7.8 8.1 10.2 11.5 12.3 T89-24 5.3 6.2 7.0 7.7 8.0 9.2 9.9 10.9 T89-25 4.6 5.0 6.0 6.7 7.0 9.0 8.9 9.3 T89-26 5.2 4.9 5.9 6.3 7.0 7.7 9.5 9.7 T89-27 4.6 5.3 6.0 6.8 7.3 9.0 9.8 10.9 T89-28 4.7 5.7 6.1 6.7 7.8 9.1 10.3 10.6 T89-29 4.5 4.9 5.7 7.4 7.3 7.6 8.5 9.4 T89-30 3.7 4.9 5.3 6.0 6.3 7.6 8.5 9.3 T89-31 5.2 6.1 7.1 8.3 8.2 9.4 10.2 10.7 T89-32 5.1 5.9 7.0 7.8 8.5 9.7 10.6 12.1 T89-33 4.9 5.6 6.7 7.8 8.6 10.0 10.3 11.1 T89-34 7.0 6.7 7.9 9.1 9.6 11.0 11.3 12.1 T89-35 4.9 5.4 6.8 7.5 8.5 11.1 10.3 10.9 T89-36 5.3 6.3 6.5 7.2 7.3 8.1 9.5 9.8 T89-37 5.0 5.7 6.4 6.9 6.9 7.7 9.2 9.1 T89-38 4.4 4.9 6.2 7.2 7.6 9.0 10.1 11.3 T89-39 4.8 5.1 6.2 6.6 7.5 8.5 9.7 10.2 T89-40 5.1 5.8 6.9 7.4 7.8 9.0 10.3 10.7 T89-41 4.2 5.3 6.1 7.2 8.0 8.9 10.4 10.5 T89-42 4.9 6.1 6.2 7.2 8.0 9.2 10.2 10.7 T89-43 4.6 5.8 6.4 7.8 7.8 9.6 11.1 11.5 T89-44 5.0 5.6 6.3 7.0 7.4 9.0 10.9 10.6 T89-45 5.0 5.1 5.4 6.8 6.2 7.9 8.7 9.3 T89-46 5.2 5.8 6.7 7.6 8.2 9.4 10.8 11.4 T89-47 4.6 5.8 6.3 7.3 8.2 9.1 9.8 10.3

[0256] Results from growth analysis are specified in the overview table 1.8c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00022 TABLE 1.8c Overview table of growth effects of construct TF0089 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0089 1.07 0.98 1.12 1.02 1.17 0.99 1.17 0.96

Construction Group TF0109

[0257] This construct induces increased growth. The final height is 24% higher comparing the average of the construction group and wild type control group. The final height is 39% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 27% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 44% higher comparing the largest individuals of the construction group and wild type control group. The TF0109 construction group meets the more stringent level of growth difference selection criteria (1), (3) and (4) as shown in table 1.9d.

[0258] Tables 1.9a and 1.9b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00023 TABLE 1.9a Height growth data for TF0109 Days in Height (cm) greenhouse 17 20 27 34 41 48 56 TF0109-1B-1 17 20 34 56 81 108 138 TF0109-1B-2 19 23 42 62 88 113 142 TF0109-2A 16 21 39 59 87 118 150 TF0109-2B 19 25 45 75 121 157 185 TF0109-3B 19 25 46 70 96 123 159 TF0109-4A 15 20 36 56 81 105 133 TF0109-4B 20 28 51 84 122 162 210 T89-01 18 20 29 46 65 87 111 T89-02 20 23 38 54 75 98 122 T89-03 16 19 32 49 72 95 124 T89-04 19 24 40 57 81 103 133 T89-05 18 22 35 52 71 95 124 T89-06 16 21 33 53 77 100 132 T89-07 18 22 37 57 82 107 138 T89-08 12 14 25 42 64 87 117 T89-09 12 16 33 52 75 101 128 T89-10 16 20 31 51 75 100 133 T89-11 12 17 31 52 75 98 122 T89-12 17 22 39 43 N/A N/A N/A T89-13 18 23 39 59 83 110 145 T89-14 19 23 40 61 81 100 127 T89-15 19 21 33 51 77 99 127 T89-16 21 24 40 57 82 107 140 T89-17 20 24 37 56 77 103 136 T89-18 19 24 39 58 83 110 140 T89-19 20 24 35 51 76 102 132 T89-20 19 23 39 59 82 111 146 T89-21 21 24 37 57 81 107 136 T89-22 20 24 39 61 85 110 142 T89-23 19 22 37 54 77 102 128 T89-24 17 22 35 55 80 107 140 T89-25 19 23 38 57 78 101 131 T89-26 18 20 35 53 71 99 128 T89-27 23 27 42 59 85 106 129 T89-28 19 25 37 55 76 96 126 T89-29 12 13 19 29 46 66 91 T89-30 19 23 36 58 81 107 136 T89-31 19 21 33 48 71 95 124 T89-32 16 20 31 48 71 97 126 T89-33 19 22 36 56 78 103 132 T89-34 18 21 36 52 74 97 120 T89-35 20 77 36 54 76 97 125 T89-36 21 28 43 65 87 115 151 T89-37 16 19 32 50 73 97 126 T89-38 17 21 35 52 74 92 113 T89-39 17 19 28 45 60 89 116 T89-40 21 26 38 55 79 104 130

TABLE-US-00024 TABLE 1.9b Diameter growth data for TF0109 Diameter (mm) Days in greenhouse 27 34 41 48 56 TF0109-1B-1 3.9 5.2 6.9 9.0 10.5 TF0109-1B-2 4.3 5.7 7.2 8.8 9.8 TF0109-2A 4.1 5.8 7.9 9.9 11.1 TF0109-2B 4.0 5.8 7.2 8.8 9.4 TF0109-3B 4.4 6.0 7.9 9.6 10.2 TF0109-4A 4.2 5.4 7.5 8.4 10.0 TF0109-4B 4.2 5.5 7.1 8.6 10.0 T89-01 3.5 4.3 5.4 7.0 8.1 T89-02 4.2 6.0 7.6 8.0 9.7 T89-03 3.6 5.9 7.0 8.0 9.9 T89-04 4.0 6.0 7.7 8.9 10.7 T89-05 4.0 5.6 7.4 9.0 10.4 T89-06 4.7 6.2 7.8 9.9 11.2 T89-07 5.3 5.8 7.0 8.4 10.0 T89-08 4.0 4.6 6.4 7.8 9.4 T89-09 4.2 4.9 7.0 9.4 10.5 T89-10 3.9 5.0 7.0 9.0 9.7 T89-11 3.8 5.0 6.6 7.7 9.1 T89-12 4.8 N/A N/A N/A N/A T89-13 4.0 5.5 7.2 8.9 10.1 T89-14 4.4 5.8 6.9 8.2 8.0 T89-15 3.6 4.6 6.5 7.4 8.7 T89-16 4.0 5.3 6.4 8.8 9.3 T89-17 3.8 6.4 6.7 8.5 9.6 T89-18 4.3 5.8 6.9 8.1 9.7 T89-19 3.7 5.0 6.9 7.9 9.3 T89-20 3.9 5.2 7.1 8.4 9.8 T89-21 4.3 5.4 7.0 8.5 11.0 T89-22 4.3 5.6 7.0 9.0 10.0 T89-23 4.0 5.2 7.3 8.6 10.8 T89-24 4.6 5.8 7.5 8.5 10.0 T89-25 4.0 5.9 6.7 7.3 8.8 T89-26 4.2 5.4 6.4 8.0 8.4 T89-27 4.2 5.1 6.5 7.5 7.7 T89-28 3.6 4.5 6.4 7.5 8.9 T89-29 N/A 2.5 3.4 4.9 5.9 T89-30 4.2 5.2 6.6 8.0 9.4 T89-31 3.8 4.9 6.5 8.3 9.7 T89-32 3.9 4.8 6.0 7.5 9.2 T89-33 3.7 5.0 7.7 7.8 9.4 T89-34 3.9 5.1 6.2 7.5 7.5 T89-35 3.6 5.5 6.7 7.6 8.9 T89-36 4.1 5.6 7.3 8.8 10.4 T89-37 3.8 4.9 6.9 7.8 9.5 T89-38 3.4 4.6 5.4 5.9 6.5 T89-39 2.8 4.0 5.4 6.2 7.2 T89-40 3.7 5.8 6.4 8.3 9.2

[0259] Real-time RT-PCR was used to confirm over-expression of construct TF0109. Table 1.9c contains gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios. 4 of 7 individuals of construction group TF0109 are over-expressed according to present RT-PCR data. 2 of 7 individuals of construction group TF0109 are down-regulated according to present RT-PCR data. Individuals having higher expression levels of construct TF0109 are correlatively tall and fast growing while individuals having lower expression levels of construct TF0109 are shorter.

TABLE-US-00025 TABLE 1.9c Real-time RT-PCR data for TF0109 Relative gene expression level Sample normalized to wild type average TF0109-1B-1 0.39 TF0109-1B-2 0.55 TF0109-2A 2.16 TF0109-2B 9.21 TF0109-3B 1.80 TF0109-4A 0.95 TF0109-4B 6.24 T89-03 1.08 T89-07 1.17 T89-08 1.11 T89-10 0.79 T89-11 0.85

[0260] Results from growth analysis are specified in the overview table 1.9d. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00026 TABLE 1.9d Overview table of growth effects of construct TF0109 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0109 1.24 1.09 1.27 1.12 1.39 1.00 1.44 1.02

Construction Group TF0132

[0261] This construct induces increased growth. The final height is 13% higher comparing the average of the construction group and wild type control group. The final height is 26% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 18% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 18% higher comparing the largest individuals of the construction group and wild type control group. The TF0132 construction group meets the more stringent level of growth difference selection criteria (1) and (4) as shown in table 1.10c.

[0262] Tables 1.10a and 1.10b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00027 TABLE 1.10a Height growth data for TF0132 Days in Height (cm) greenhouse 20 28 34 37 41 48 51 55 59 TF0132-3A-1 23 38 53 60 70 89 96 107 119 TF0132-3A-2 15 28 42 49 58 75 81 93 100 TF0132-3A-3 26 41 54 61 72 89 96 107 119 TF0132-3B-1 27 47 63 73 84 113 125 142 156 TF0132-3B-2 30 51 71 80 94 N/A 134 148 160 TF0132-3BB 32 58 87 96 112 141 155 171 182 TF0132-4AA 20 39 59 67 78 102 112 129 143 TF0132-4AB 21 43 63 74 87 114 126 142 156 TF0132-4AC 27 48 67 76 89 117 129 146 156 T89-02 27 40 51 58 66 86 94 105 115 T89-03 27 44 58 67 77 97 104 114 126 T89-04 27 39 56 65 74 92 100 109 118 T89-07 28 47 66 77 88 107 116 130 140 T89-08 19 37 51 59 69 90 99 109 119 T89-09 25 43 58 67 78 100 109 121 131 T89-11 29 45 60 69 81 104 111 121 132 T89-12 26 41 58 65 77 105 114 127 136 T89-13 31 47 70 78 90 110 119 131 143 T89-14 26 46 70 78 88 108 116 127 137 T89-18 22 41 55 65 74 95 104 115 126 T89-19 27 43 60 70 81 102 110 121 131 T89-20 25 40 57 N/A 74 94 103 118 130 T89-21 27 45 61 72 84 108 115 126 137 T89-22 25 38 55 65 77 95 104 115 125 T89-23 23 37 50 57 65 82 92 106 115 T89-24 25 41 54 60 69 87 94 102 110 T89-25 25 38 55 66 80 100 109 121 133 T89-26 24 38 53 61 69 81 87 95 104 T89-27 26 39 54 62 70 90 97 109 120 T89-28 27 46 67 75 86 107 114 126 136 T89-29 24 39 57 64 74 91 98 108 128 T89-31 27 43 57 65 73 91 97 105 113 T89-32 25 41 55 63 75 96 107 121 135 T89-35 25 38 53 62 73 88 96 103 112 T89-36 27 45 67 78 90 113 122 134 145 T89-37 20 43 59 68 79 101 112 124 137 T89-39 25 45 61 69 79 99 108 120 132 T89-40 23 32 43 49 60 76 84 94 104 T89-41 27 45 66 75 87 112 120 133 144 T89-43 26 38 54 61 70 93 100 109 120 T89-46 26 45 66 76 88 109 114 125 133

TABLE-US-00028 TABLE 1.10b Diameter growth data for TF0132 Diameter (mm) Days in greenhouse 34 41 48 55 59 TF0132-3A-1 6.1 7.3 7.8 9.0 8.9 TF0132-3A-2 5.8 7.3 7.8 9.0 9.0 TF0132-3A-3 6.6 8.5 8.6 9.2 9.7 TF0132-3B-1 6.2 7.0 8.6 9.2 9.7 TF0132-3B-2 5.7 7.3 8.8 9.8 9.2 TF0132-3BB 6.4 7.3 8.0 9.1 9.2 TF0132-4AA 5.3 6.0 7.4 8.8 9.1 TF0132-4AB 5.6 7.0 7.0 8.4 8.4 TF0132-4AC 6.2 7.2 9.1 9.6 10.0 T89-02 5.6 6.4 7.8 9.0 8.4 T89-03 6.6 7.7 8.5 8.9 9.6 T89-04 7.2 7.8 9.1 9.8 9.7 T89-07 7.3 7.9 9.5 9.9 10.1 T89-08 5.5 7.8 8.3 10.0 9.8 T89-09 6.7 7.8 9.3 9.7 9.8 T89-11 6.4 8.1 9.2 9.4 9.7 T89-12 7.0 8.0 9.0 10.1 9.9 T89-13 6.8 8.5 9.1 10.7 10.8 T89-14 6.1 6.9 7.7 8.4 8.8 T89-18 5.5 6.5 7.9 9.5 9.4 T89-19 7.5 8.3 9.0 9.5 10.4 T89-20 7.3 7.1 8.7 9.3 9.7 T89-21 7.0 8.6 9.3 10.3 10.3 T89-22 7.0 7.8 8.4 10.0 9.5 T89-23 5.8 6.8 7.9 9.9 9.7 T89-24 6.1 6.8 8.1 9.0 9.4 T89-25 6.5 8.8 9.5 10.0 10.9 T89-26 5.8 6.7 7.0 7.5 7.7 T89-27 6.3 7.4 8.6 9.0 9.5 T89-28 7.0 8.1 8.8 9.7 10.5 T89-29 7.5 7.4 8.5 10.7 10.0 T89-31 5.6 6.3 7.2 7.6 8.6 T89-32 6.0 7.0 8.2 8.7 9.3 T89-35 5.4 6.4 6.8 7.9 8.0 T89-36 6.7 8.2 8.9 9.6 9.4 T89-37 7.4 8.5 8.6 9.3 10.8 T89-39 7.1 7.9 9.6 10.2 10.2 T89-40 3.9 5.2 5.7 6.8 6.6 T89-41 7.2 8.7 N/A 9.7 10.3 T89-43 6.9 7.5 8.6 9.7 10.4 T89-46 6.4 7.2 8.2 8.7 9.7

[0263] Results from growth analysis are specified in the overview table 1.10c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00029 TABLE 1.10c Overview table of growth effects of construct TF0132 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0132 1.13 0.97 1.18 1.04 1.26 0.92 1.18 0.99

Construction Group TFSTT051

[0264] This construct induces increased growth. The final height is 7% higher comparing the average of the construction group and wild type control group. The final height is 11% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 5% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 10% higher comparing the largest individuals of the construction group and wild type control group. The TFSTT051 construction group meets growth difference selection criterion (1) as shown in table 1.11d.

[0265] Tables 1.11a and 1.11b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00030 TABLE 1.11a Height growth data for TFSTT051 Days in Height (cm) greenhouse 19 28 34 40 44 47 51 54 61 65 TFSTT051-1B 13 24 43 57 69 76 83 89 104 112 TFSTT051-2A 18 33 48 61 72 81 93 103 120 131 TFSTT051-2B 17 28 44 56 67 76 88 96 117 129 TFSTT051-3A 19 31 46 59 70 77 87 96 120 130 TFSTT051-3B 16 33 52 67 79 90 101 112 134 145 TFSTT051-4A 18 36 57 71 86 98 111 119 140 154 TFSTT051-4B-1 18 34 52 67 81 87 96 103 123 136 TFSTT051-4B-2 17 31 51 66 78 85 95 105 124 135 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

TABLE-US-00031 TABLE 1.11b Diameter growth data for TFSTT051 Diameter (mm) Days in greenhouse 28 34 40 44 47 51 54 61 65 TFSTT051-1B 3.2 4.6 5.6 6.4 6.6 7.3 7.2 7.8 8.0 TFSTT051-2A 3.2 4.5 5.7 6.7 7.4 8.6 9.3 8.8 8.9 TFSTT051-2B 3.1 4.4 6.0 6.0 6.8 8.0 8.2 9.0 9.2 TFSTT051-3A 3.5 5.0 6.1 6.6 7.1 8.2 8.7 9.8 10.5 TFSTT051-3B 4.2 5.6 6.8 8.0 8.5 9.4 9.8 10.2 11.1 TFSTT051-4A 4.5 5.9 7.1 8.8 8.7 9.4 10.1 11.1 11.1 TFSTT051-4B-1 4.1 5.6 6.8 7.9 8.1 8.6 9.7 10.7 11.6 TFSTT051-4B-2 3.9 5.5 6.3 7.6 8.2 9.4 8.8 9.6 10.3 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 N/A 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[0266] Real-time RT-PCR was used to confirm over-expression of construct TFSTT051. Table 1.11c contains gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios. 1 of 8 individuals of construction group TFSTT051 are over-expressed according to present RT-PCR data.

TABLE-US-00032 TABLE 1.11c Real-time RT-PCR data for TFSTT051 Relative gene expression level Sample normalized to wild type average TFSTT051-1B 1.18 TFSTT051-2A 7.42 TFSTT051-2B 1.13 TFSTT051-3A 0.30 TFSTT051-3B 1.06 TFSTT051-4A 0.65 TFSTT051-4B-1 1.34 TFSTT051-4B-2 0.76 T89-11 0.42 T89-12 1.65 T89-24 1.27 T89-25 0.66

[0267] Results from growth analysis are specified in the overview table 1.11d. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00033 TABLE 1.11d Overview table of growth effects of construct TFSTT051 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT051 1.07 1.06 1.05 1.01 1.11 0.94 1.10 0.90

Construction Group TF0013

[0268] This construct induces increased growth. The final height is 12% higher comparing the average of the construction group and wild type control group. The final height is 6% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 20% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 33% higher comparing the largest individuals of the construction group and wild type control group. The TF0013 construction group meets the more stringent level of growth difference selection criterion (4) and the less stringent level of growth difference selection criterion (1) and (3) as shown in table 1.12d.

[0269] Tables 1.12a and 1.12b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00034 TABLE 1.12a Height growth data for TF0013 Days in Height (cm) greenhouse 18 22 25 29 32 39 43 46 50 53 57 TF0013-1A-1 19 24 31 43 53 81 98 111 128 144 161 TF0013-1A-2 12 15 19 27 37 62 77 90 108 125 159 TF0013-2A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A TF0013-2B 20 25 32 45 57 85 102 112 128 142 157 TF0013-3A 19 25 33 47 58 88 103 116 134 151 170 TF0013-3BA 24 30 37 52 64 94 112 125 144 159 179 TF0013-3BB 19 26 31 43 53 84 100 114 130 144 164 TF0013-4BA 25 31 40 52 63 89 107 117 130 141 155 TF0013-4BB 20 25 31 45 57 83 100 111 128 145 162 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

TABLE-US-00035 TABLE 1.12b Diameter growth data for TF0013 Days in Diameter (mm) greenhouse 29 32 39 43 46 50 53 57 TF0013-1A-1 3.9 5.1 6.6 7.6 8.6 9.8 9.9 10.5 TF0013-1A-2 3.0 2.8 5.5 6.5 7.2 8.3 8.6 8.6 TF0013-2A N/A N/A N/A N/A N/A N/A N/A N/A TF0013-2B 4.3 5.0 6.4 7.4 8.0 8.5 9.2 10.2 TF0013-3A 4.6 5.4 7.1 8.2 8.7 9.7 10.3 10.7 TF0013-3BA 4.4 5.0 7.2 7.5 9.1 9.1 9.8 10.6 TF0013-3BB 3.8 5.2 6.7 7.5 7.5 8.4 9.0 9.8 TF0013-4BA 4.7 5.7 6.1 6.8 7.4 8.8 9.0 9.6 TF0013-4BB 3.8 4.9 N/A 8.4 8.3 9.0 9.6 9.9 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 N/A 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7 7.5 8.6 9.4 9.8

[0270] Real-time RT-PCR was used to confirm over-expression of construct TF0013. Table 1.12c contains gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios. 3 of 8 individuals of construction group TF0013 are over-expressed according to present RT-PCR data.

TABLE-US-00036 TABLE 1.12c Real-time RT-PCR data for TF0013 Relative gene expression level Sample normalized to wild type average TF0013-1A-1 0.59 TF0013-1A-2 1.02 TF0013-2B 2.32 TF0013-3A 0.90 TF0013-3BA 0.86 TF0013-3BB 0.80 TF0013-4BA 1.38 TF0013-4BB 1.65 T89-03 1.22 T89-36 1.04 T89-37 0.90 T89-38 0.85

[0271] Results from growth analysis are specified in the overview table 1.12d. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00037 TABLE 1.12d Overview table of growth effects of construct TF0013 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0013 1.12 1.07 1.20 1.12 1.06 1.02 1.33 1.01

1.3.10 Construction Group TF0097

[0272] This construct induces increased growth. The final height is 10% higher comparing the average of the construction group and wild type control group. The final height is 16% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 15% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 15% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 7% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 21% higher comparing the largest individuals of the construction group and wild type control group. The TF0097 construction group meets the more stringent level of growth difference selection criterion (1) and the less stringent level of growth difference selection criterion (4) as shown in table 1.13d.

[0273] Tables 1.13a and 1.13b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00038 TABLE 1.13a Height growth data for TF0097 Height (cm) Days in greenhouse 16 20 29 34 41 48 TF0097-1A 28 35 71 93 133 176 TF0097-1B 26 36 72 100 137 171 TF0097-2A 19 22 42 57 79 101 TF0097-2B 31 40 79 105 138 175 TF0097-3A-1 28 36 73 96 130 166 TF0097-3A-2 30 38 72 100 141 178 TF0097-4A-1 27 32 48 68 103 140 TF0097-4A-2 28 34 65 85 118 141 TF0097-4B 25 10 23 37 65 92 T89-01 28 35 66 89 122 150 T89-02 25 35 62 80 103 128 T89-03 24 33 61 83 113 142 T89-04 27 34 65 87 120 152 T89-05 27 32 59 80 107 138 T89-06 25 32 61 77 106 135 T89-07 22 28 50 67 92 120 T89-08 24 29 56 75 103 122 T89-09 20 24 45 61 91 115 T89-10 22 28 52 70 99 124 T89-11 26 32 63 80 110 142 T89-12 27 32 60 80 109 132 T89-13 23 30 59 79 107 133 T89-14 26 31 60 79 106 131 T89-15 26 32 58 79 110 133 T89-16 29 32 65 87 121 151 T89-17 25 32 59 79 105 133 T89-18 29 37 67 86 114 136 T89-19 29 35 66 84 111 129 T89-20 27 38 64 83 112 122 T89-21 28 33 58 79 108 132 T89-22 25 33 54 83 113 140 T89-23 24 31 56 75 104 134 T89-24 25 33 58 78 106 136 T89-25 26 33 62 80 111 142 T89-26 28 35 64 81 113 146 T89-27 26 31 60 75 107 140 T89-28 28 34 61 83 116 147 T89-29 19 24 49 70 101 132 T89-30 26 33 57 77 110 140 T89-31 25 35 63 81 115 140 T89-32 28 36 67 89 121 151 T89-33 26 33 63 85 110 126 T89-34 28 35 59 77 107 135 T89-35 26 34 59 77 107 132 T89-36 26 32 61 79 107 136 T89-37 22 23 39 54 78 106 T89-38 27 35 58 74 98 124 T89-39 24 29 53 73 103 128 T89-40 22 27 52 70 101 121 T89-41 27 34 64 81 111 143 T89-42 25 33 63 79 111 142 T89-43 26 33 62 84 117 148 T89-44 26 32 64 82 115 149 T89-45 24 33 60 79 107 134 T89-46 27 34 64 88 121 147 T89-47 24 32 61 78 101 121 T89-48 24 31 62 79 108 140 T89-49 27 35 63 80 109 138 T89-50 26 33 61 80 108 135 T89-51 20 25 52 70 98 116 T89-52 24 28 53 73 99 128 T89-53 21 31 58 75 100 115 T89-54 22 30 54 72 105 133 T89-55 21 27 53 73 105 129 T89-56 29 36 67 84 115 147 T89-57 27 32 60 80 111 139 T89-58 29 38 67 86 119 150 T89-59 25 32 63 86 120 149 T89-60 27 33 62 84 119 154 T89-61 26 35 65 89 124 152 T89-62 24 30 57 79 107 134 T89-63 25 33 62 81 111 134 T89-64 21 29 58 79 110 139 T89-65 23 31 55 70 96 122 T89-66 25 32 61 82 113 142 T89-67 25 30 50 75 105 131 T89-68 22 31 61 80 110 138 T89-69 25 33 56 74 101 128 T89-70 21 28 59 77 105 135 T89-71 20 26 57 73 101 129 T89-72 26 33 62 82 115 144

TABLE-US-00039 TABLE 1.13c Diameter growth data for TF0097 Diameter (mm) Days in greenhouse 29 34 41 48 TF0097-1A 4.7 5.0 6.9 8.2 TF0097-1B 4.2 5.1 6.9 8.2 TF0097-2A 3.0 3.6 4.1 7.9 TF0097-2B 5.9 6.6 7.1 8.6 TF0097-3A-1 4.9 5.9 7.0 8.1 TF0097-3A-2 5.2 6.4 8.1 9.3 TF0097-4A-1 3.9 5.7 5.0 6.2 TF0097-4A-2 4.7 4.8 5.6 6.4 TF0097-4B 2.5 2.9 4.0 4.8 T89-01 5.3 6.3 8.0 8.8 T89-02 4.8 5.3 6.7 7.1 T89-03 5.3 6.4 8.2 8.7 T89-04 5.3 6.5 7.9 9.3 T89-05 5.4 6.5 7.6 8.1 T89-06 5.3 6.0 7.2 8.4 T89-07 4.0 4.8 7.2 7.7 T89-08 4.5 5.4 6.3 7.1 T89-09 3.7 4.7 6.1 7.5 T89-10 4.4 5.5 6.9 7.6 T89-11 5.4 6.4 7.1 8.0 T89-12 5.5 5.7 6.8 8.1 T89-13 5.2 6.0 6.7 7.4 T89-14 5.3 6.4 6.7 7.9 T89-15 5.1 6.4 6.9 7.8 T89-16 5.2 6.4 7.6 9.2 T89-17 5.0 5.7 6.5 7.9 T89-18 5.8 6.4 7.1 7.7 T89-19 5.4 6.4 7.5 8.0 T89-20 5.6 6.6 8.3 8.6 T89-21 5.0 5.8 6.9 7.8 T89-22 5.4 6.5 8.2 8.9 T89-23 4.7 5.3 7.1 8.0 T89-24 4.8 6.3 6.9 8.4 T89-25 5.0 6.3 6.9 7.8 T89-26 5.6 6.6 7.5 8.5 T89-27 5.5 6.0 7.5 8.8 T89-28 6.0 7.1 7.7 8.8 T89-29 4.2 5.3 6.8 8.5 T89-30 5.5 6.4 8.0 9.3 T89-31 5.7 5.7 8.4 8.9 T89-32 5.4 6.5 7.8 9.0 T89-33 5.6 6.3 6.8 7.4 T89-34 5.1 6.1 7.2 8.1 T89-35 5.6 6.7 7.7 8.2 T89-36 5.4 6.2 7.7 9.4 T89-37 2.8 3.3 4.7 5.5 T89-38 5.1 5.5 7.1 8.4 T89-39 4.9 5.5 6.5 7.4 T89-40 4.9 6.4 7.6 7.6 T89-41 5.6 7 7.3 9.1 T89-42 5.9 6.4 7.7 8.4 T89-43 5.4 6.7 8.2 9.1 T89-44 6.4 6.4 8.1 9.3 T89-45 5.6 6.4 7.8 8.0 T89-46 6.1 6.4 7.9 9.5 T89-47 5.2 5.9 6.5 6.9 T89-48 5.1 6.1 7.3 8.8 T89-49 5.3 5.9 7.3 7.7 T89-50 5.3 6.8 7.7 8.2 T89-51 4.7 5.7 7 7.5 T89-52 4.9 5.5 6.9 8.2 T89-53 5.1 6.1 6.8 7.5 T89-54 4.7 5.9 6.7 7.7 T89-55 4.8 5.9 7.1 7.9 T89-56 5.4 6.9 7.7 9.1 T89-57 5.3 6.5 7.5 9.2 T89-58 5.5 6.5 8 8.8 T89-59 5.8 6.4 7.7 8.4 T89-60 5.6 6.2 7.9 9.6 T89-61 5.6 7 8.3 9.4 T89-62 5.1 6.3 7.2 7.7 T89-63 5.5 7.1 8 8.4 T89-64 4.7 6.2 8.4 8.1 T89-65 5.3 6.1 6.9 8.2 T89-66 5.2 6 7.2 8.4 T89-67 5.8 6.5 7.7 8.9 T89-68 5 6.1 7.5 8.2 T89-69 5.1 6.4 7.2 9.0 T89-70 5 6.1 6.6 7.4 T89-71 4.9 6.4 7.2 8.7 T89-72 5.8 6.8 7.9 9.1

[0274] Real-time RT-PCR was used to confirm over-expression of construct TF0097. Table 1.13c contains gene expression levels of construct gene relative to reference gene expression. All ratios between construct and reference gene expression levels shown are normalized to the average of wild type group ratios. 2 of 9 individuals of construction group TF0097 are over-expressed according to present RT-PCR data.

TABLE-US-00040 TABLE 1.13c Real-time RT-PCR data for TF0097 Relative gene expression level Sample normalized to wild type average TF0097-1A 1.85 TF0097-1B 1.66 TF0097-2A 1.45 TF0097-2B 1.57 TF0097-3A-1 1.09 TF0097-3A-2 1.71 TF0097-4A-1 0.79 TF0097-4A-2 4.64 TF0097-4B 3.20 T89-03 1.98 T89-17 0.38 T89-19 0.64

[0275] Results from growth analysis are specified in the overview table 1.13d. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00041 TABLE 1.13d Overview table of growth effects of construct TF0097 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0097 1.10 0.91 1.15 1.07 1.16 0.97 1.15 1.21

1.3.11 Construction Group TFSTT019

[0276] This construct induces increased growth. The final diameter is 11% higher comparing the average of the construction group and wild type control group. The final diameter is 8% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 18% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 9% higher comparing the largest individuals of the construction group and wild type control group. The TFSTT019 construction group meets growth difference selection criterion (2) as shown in table 1.14c.

[0277] Tables 1.14a and 1.14b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00042 TABLE 1.14a Height growth data for TFSTT019 Days in Height (cm) greenhouse 18 22 25 29 32 39 43 46 50 53 57 TFSTT019-1A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A TFSTT019-1BA 18 22 29 39 49 73 84 91 103 115 129 TFSTT019-1BB 21 26 34 46 55 82 99 107 120 132 145 TFSTT019-2A 24 30 37 51 61 88 104 114 128 140 157 TFSTT019-2B 11 14 18 28 37 60 73 86 102 115 132 TFSTT019-3A 23 31 37 50 57 81 94 106 119 129 144 TFSTT019-4BA 27 32 39 49 58 84 99 110 124 137 152 TFSTT019-4BB 21 27 35 47 57 84 99 110 124 137 151 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

TABLE-US-00043 TABLE 1.14b Diameter growth data for TFSTT019 Days in Diameter (mm) greenhouse 29 32 39 43 46 50 53 57 TFSTT019-1A N/A N/A N/A N/A N/A N/A N/A N/A TFSTT019-1BA 3.7 5.0 7.1 7.9 8.4 8.6 9.7 10.2 TFSTT019-1BB 4.0 4.7 6.4 7.3 8.0 8.7 9.2 9.4 TFSTT019-2A 4.5 5.1 7.2 7.8 9.0 9.8 10.2 10.7 TFSTT019-2B 3.5 4.2 6.0 7.1 7.6 8.5 9.0 9.1 TFSTT019-3A 4.1 5.1 6.9 8.4 9.0 10.5 10.8 11.1 TFSTT019-4BA 4.4 5.5 7.3 8.4 9.2 9.5 10.1 11.3 TFSTT019-4BB 4.5 5.7 7.1 8.1 8.4 9.5 10.2 10.6 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 N/A 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7 7.5 8.6 9.4 9.8

[0278] Results from growth analysis are specified in the overview table 1.14c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00044 TABLE 1.14c Overview table of growth effects of construct TFSTT019 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT019 0.99 1.11 0.96 1.18 0.93 1.08 0.89 1.09

1.3.12 Construction Group TFSTT035

[0279] This construct induces increased growth. The final diameter is 8% higher comparing the average of the construction group and wild type control group. The final diameter is 11% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 12% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 8% higher comparing the largest individuals of the construction group and wild type control group. The TFSTT035 construction group meets growth difference selection criterion (2) as shown in table 1.15c.

[0280] Tables 1.15a and 1.15b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00045 TABLE 1.15a Height growth data for TFSTT035 Days in Height (cm) greenhouse 18 22 25 29 32 39 43 46 50 53 57 TFSTT035-1A 23 30 36 49 60 88 101 112 127 141 158 TFSTT035-1BA 21 27 34 47 58 83 97 109 125 138 150 TFSTT035-1BB 23 28 35 47 56 80 94 105 118 129 143 TFSTT035-2AA 22 28 36 49 59 84 98 107 122 135 150 TFSTT035-2AB 20 25 31 41 49 70 83 95 109 121 137 TFSTT035-2B 18 23 29 40 51 75 90 100 112 125 138 TFSTT035-3B 20 26 31 42 52 77 93 103 116 129 145 TFSTT035-4B 14 19 25 35 44 73 88 98 113 127 141 T89-01 14 17 22 31 40 64 76 88 102 118 135 T89-02 17 20 25 36 45 69 79 90 104 118 133 T89-03 21 29 35 47 57 81 94 104 119 132 147 T89-04 13 14 17 25 33 45 52 57 70 79 92 T89-05 15 18 24 33 42 69 83 94 106 120 137 T89-06 18 23 29 40 51 78 91 103 120 134 152 T89-07 20 24 30 41 51 74 88 96 109 121 130 T89-08 27 32 40 52 62 88 99 111 124 134 148 T89-09 23 27 34 46 56 83 97 106 122 132 149 T89-10 13 15 20 27 37 59 72 83 98 111 128 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-12 26 33 40 54 66 92 107 120 135 149 166 T89-13 21 26 34 45 55 79 94 105 122 134 150 T89-14 18 23 31 42 53 80 97 107 120 128 145 T89-15 26 33 40 53 63 88 103 111 125 138 154 T89-16 27 32 39 51 63 89 104 116 132 146 162 T89-17 17 21 27 38 45 72 87 97 110 124 139 T89-18 23 30 35 46 55 80 94 105 117 131 145 T89-19 23 29 35 45 54 78 91 102 114 125 137 T89-20 22 28 34 49 60 87 100 110 125 137 155 T89-21 27 31 35 42 48 66 77 87 100 110 124 T89-22 13 16 23 32 48 67 81 92 105 118 134 T89-23 23 29 35 48 58 83 97 107 122 136 154 T89-24 23 29 36 48 59 86 101 113 128 141 159 T89-25 16 21 28 40 51 80 97 107 122 137 153 T89-26 27 35 40 54 66 93 107 118 132 144 162 T89-27 26 31 38 49 59 82 96 106 120 131 146 T89-28 25 29 36 49 59 83 97 106 119 131 147 T89-29 26 32 38 51 62 88 103 113 128 142 159 T89-30 24 29 36 48 56 80 93 104 117 131 145 T89-31 24 31 36 47 57 81 94 105 117 131 144 T89-32 26 32 38 49 58 83 98 107 120 134 148 T89-33 24 29 36 48 57 85 100 112 124 134 149 T89-34 21 26 32 43 53 79 90 102 117 131 148 T89-35 21 28 36 46 55 80 94 105 120 133 149 T89-36 16 21 28 39 51 74 88 100 115 128 144 T89-37 28 35 41 52 63 88 101 111 125 137 154 T89-38 27 32 39 51 62 86 101 113 128 140 155 T89-39 21 26 33 46 55 83 101 112 126 139 156 T89-40 21 26 33 45 55 77 88 99 113 124 139 T89-41 13 16 23 32 42 68 85 95 107 118 133 T89-42 24 30 37 49 61 86 101 113 128 143 150 T89-43 25 31 38 51 61 86 102 114 130 144 163 T89-44 23 31 39 51 64 90 105 118 135 151 169 T89-45 26 32 37 49 58 85 100 110 124 137 153 T89-46 20 25 34 43 55 81 97 109 122 133 149

TABLE-US-00046 TABLE 1.15b Diameter growth data for TFSTT035 Days in Diameter (mm) greenhouse 29 32 39 43 46 50 53 57 TFSTT035-1A 4.6 5.6 7.0 8.3 8.7 8.8 9.1 10.4 TFSTT035-1BA 4.5 5.7 7.3 8.8 9.0 10.0 11.0 11.7 TFSTT035-1BB 4.1 5.3 6.5 7.1 7.5 8.3 9.0 9.5 TFSTT035-2AA 4.1 5.0 7.0 8.0 8.7 9.2 10.0 10.3 TFSTT035-2AB 4.0 4.9 6.5 7.5 8.0 8.6 9.2 9.8 TFSTT035-2B 3.6 5.0 6.6 7.3 8.0 8.3 8.9 9.5 TFSTT035-3B 3.5 4.2 5.8 6.8 7.2 8.0 8.9 9.5 TFSTT035-4B 3.4 4.5 5.5 7.0 7.4 7.7 9.0 9.5 T89-01 3.2 3.7 5.3 6.2 6.9 7.9 8.3 8.7 T89-02 3.2 3.9 5.1 6.4 7.0 7.6 8.6 8.6 T89-03 4.2 5.3 6.4 6.9 6.9 N/A 8.8 9.8 T89-04 2.2 3.0 4.2 4.5 5.4 5.0 5.4 6.1 T89-05 3.0 3.7 4.8 5.9 6.4 7.0 7.6 7.9 T89-06 3.6 4.8 6.5 7.6 8.7 9.1 9.6 10.1 T89-07 3.8 4.9 6.8 7.6 8.0 8.6 9.0 9.7 T89-08 4.4 5.8 6.2 7.3 7.6 8.5 9.4 9.7 T89-09 4.5 5.5 6.5 7.1 7.6 9.1 9.4 9.8 T89-10 3.7 3.9 5.0 6.1 6.8 7.6 8.5 9.2 T89-11 N/A N/A N/A N/A N/A N/A N/A N/A T89-12 4.6 5.5 7.3 8.6 8.8 9.6 10.2 10.2 T89-13 3.8 4.6 5.7 6.5 6.6 7.4 8.1 8.6 T89-14 3.7 4.2 5.3 5.6 6.1 7.0 7.1 7.8 T89-15 4.6 5.0 6.4 7.0 7.4 8.0 8.4 9.1 T89-16 5.0 5.5 7.0 7.6 8.0 8.6 9.0 9.7 T89-17 3.1 4.3 5.7 7.1 7.4 7.9 8.4 9.2 T89-18 4.5 5.3 7.8 8.0 8.1 9.2 9.9 10.3 T89-19 4.2 5.8 6.7 8.2 8.5 8.5 9.0 9.5 T89-20 4.0 4.7 6.7 8.1 7.6 8.5 8.6 9.4 T89-21 3.5 3.9 5.0 5.7 5.8 7.1 6.7 7.4 T89-22 3.6 4.3 5.5 6.3 6.8 7.9 8.4 8.8 T89-23 4.1 5.2 7.0 7.6 8.2 8.9 9.2 10.0 T89-24 4.1 5.4 6.7 7.5 7.8 8.6 9.6 10.4 T89-25 3.7 4.6 6.0 7.0 7.7 8.3 8.5 9.4 T89-26 4.8 5.6 6.9 7.3 7.8 8.6 9.2 9.5 T89-27 3.8 4.9 6.2 6.7 6.9 7.6 8.4 9.3 T89-28 4.5 5.5 6.8 7.3 7.8 9.1 9.1 9.5 T89-29 4.4 5.3 6.8 6.8 7.4 8.2 9.2 8.9 T89-30 3.7 4.7 5.4 6.6 7.0 7.0 7.7 8.5 T89-31 4.0 4.5 5.5 6.9 7.0 8.0 9.1 9.6 T89-32 3.6 4.5 5.7 7.0 7.2 8.0 9.1 9.4 T89-33 3.9 4.6 6.7 7.3 8.0 8.6 9.4 10.5 T89-34 3.6 4.5 5.9 6.9 7.5 8.2 9.1 9.4 T89-35 3.9 4.5 5.7 7.1 7.5 7.9 8.8 9.6 T89-36 3.6 5.0 5.7 6.5 6.8 7.9 8.2 9.1 T89-37 4.3 5.6 7.1 8.0 8.0 8.9 9.7 10.2 T89-38 4.7 5.8 6.7 7.8 8.0 8.7 9.2 9.7 T89-39 4.2 5.0 6.2 7.3 8.2 8.1 8.7 9.6 T89-40 3.6 4.4 5.2 5.7 6.0 7.2 7.2 8.0 T89-41 3.5 4.3 5.5 6.5 7.0 7.5 7.7 8.4 T89-42 4.4 5.1 7.5 8.4 9.5 9.9 10.0 10.3 T89-43 4.3 5.0 6.5 7.3 7.8 8.3 8.9 9.1 T89-44 4.3 5.8 6.8 8.1 8.6 9.5 9.9 10.5 T89-45 4.2 4.9 6.8 7.5 7.6 8.5 9.4 9.7 T89-46 3.5 4.4 5.7 7 7.5 8.6 9.4 9.8

[0281] Results from growth analysis are specified in the overview table 1.15c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00047 TABLE 1.15c Overview table of growth effects of construct TFSTT035 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT035 0.99 1.08 0.98 1.12 0.93 1.11 0.90 1.08

1.3.13 Construction Group TFSTT047

[0282] This construct induces increased growth. The final diameter is 8% higher comparing the average of the construction group and wild type control group. The final diameter is 11% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 12% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 8% higher comparing the largest individuals of the construction group and wild type control group. The TFSTT047 construction group meets growth difference selection criterion (3) as shown in table 1.16c.

[0283] Tables 1.16a and 1.16b contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00048 TABLE 1.16a Height growth data for TFSTT047 Days in Height (cm) greenhouse 20 28 34 37 41 48 51 55 59 TFSTT047-1B 29 44 56 64 73 91 99 111 121 TFSTT047-2A 29 47 67 76 87 114 122 135 147 TFSTT047-2B 26 44 67 76 90 116 125 139 150 TFSTT047-3B 25 43 65 74 87 109 118 131 143 TFSTT047-4A 25 45 68 75 87 108 115 128 138 T89-02 27 40 51 58 66 86 94 105 115 T89-03 27 44 58 67 77 97 104 114 126 T89-04 27 39 56 65 74 92 100 109 118 T89-07 28 47 66 77 88 107 116 130 140 T89-08 19 37 51 59 69 90 99 109 119 T89-09 25 43 58 67 78 100 109 121 131 T89-11 29 45 60 69 81 104 111 121 132 T89-12 26 41 58 65 77 105 114 127 136 T89-13 31 47 70 78 90 110 119 131 143 T89-14 26 46 70 78 88 108 116 127 137 T89-18 22 41 55 65 74 95 104 115 126 T89-19 27 43 60 70 81 102 110 121 131 T89-20 25 40 57 N/A 74 94 103 118 130 T89-21 27 45 61 72 84 108 115 126 137 T89-22 25 38 55 65 77 95 104 115 125 T89-23 23 37 50 57 65 82 92 106 115 T89-24 25 41 54 60 69 87 94 102 110 T89-25 25 38 55 66 80 100 109 121 133 T89-26 24 38 53 61 69 81 87 95 104 T89-27 26 39 54 62 70 90 97 109 120 T89-28 27 46 67 75 86 107 114 126 136 T89-29 24 39 57 64 74 91 98 108 128 T89-31 27 43 57 65 73 91 97 105 113 T89-32 25 41 55 63 75 96 107 121 135 T89-35 25 38 53 62 73 88 96 103 112 T89-36 27 45 67 78 90 113 122 134 145 T89-37 20 43 59 68 79 101 112 124 137 T89-39 25 45 61 69 79 99 108 120 132 T89-40 23 32 43 49 60 76 84 94 104 T89-41 27 45 66 75 87 112 120 133 144 T89-43 26 38 54 61 70 93 100 109 120 T89-46 26 45 66 76 88 109 114 125 133

TABLE-US-00049 TABLE 1.16b Diameter growth data for TFSTT047 Diameter (mm) Days in greenhouse 34 41 48 55 59 TFSTT047-1B 6.1 7.0 8.3 9.3 9.4 TFSTT047-2A 6.7 7.6 8.7 10.0 10.4 TFSTT047-2B 6.9 7.5 N/A 9.5 10.6 TFSTT047-3B 6.8 7.8 8.9 9.9 10.1 TFSTT047-4A 5.7 6.9 8.2 9.1 9.8 T89-02 5.6 6.4 7.8 9.0 8.4 T89-03 6.6 7.7 8.5 8.9 9.6 T89-04 7.2 7.8 9.1 9.8 9.7 T89-07 7.3 7.9 9.5 9.9 10.1 T89-08 5.5 7.8 8.3 10.0 9.8 T89-09 6.7 7.8 9.3 9.7 9.8 T89-11 6.4 8.1 9.2 9.4 9.7 T89-12 7.0 8.0 9.0 10.1 9.9 T89-13 6.8 8.5 9.1 10.7 10.8 T89-14 6.1 6.9 7.7 8.4 8.8 T89-18 5.5 6.5 7.9 9.5 9.4 T89-19 7.5 8.3 9.0 9.5 10.4 T89-20 7.3 7.1 8.7 9.3 9.7 T89-21 7.0 8.6 9.3 10.3 10.3 T89-22 7.0 7.8 8.4 10.0 9.5 T89-23 5.8 6.8 7.9 9.9 9.7 T89-24 6.1 6.8 8.1 9.0 9.4 T89-25 6.5 8.8 9.5 10.0 10.9 T89-26 5.8 6.7 7.0 7.5 7.7 T89-27 6.3 7.4 8.6 9.0 9.5 T89-28 7.0 8.1 8.8 9.7 10.5 T89-29 7.5 7.4 8.5 10.7 10.0 T89-31 5.6 6.3 7.2 7.6 8.6 T89-32 6.0 7.0 8.2 8.7 9.3 T89-35 5.4 6.4 6.8 7.9 8.0 T89-36 6.7 8.2 8.9 9.6 9.4 T89-37 7.4 8.5 8.6 9.3 10.8 T89-39 7.1 7.9 9.6 10.2 10.2 T89-40 3.9 5.2 5.7 6.8 6.6 T89-41 7.2 8.7 N/A 9.7 10.3 T89-43 6.9 7.5 8.6 9.7 10.4 T89-46 6.4 7.2 8.2 8.7 9.7

[0284] Results from growth analysis are specified in the overview table 1.16c. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00050 TABLE 1.16c Overview table of growth effects of construct TFSTT047 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT047 1.10 1.05 1.08 1.20 1.03 0.97 0.99 1.00

Example 2

Construction Group TF0002Rp2

[0285] This construct induces increased growth. The final height is 29% higher comparing the average of the construction group and wild type control group. The final height is 27% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 36% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 38% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 10% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 9% higher comparing the largest individuals of the construction group and wild type control group. The TF0002Rp2 construction group meets the more stringent level of growth difference selection criteria (1), (3) and (4) as shown in table 2.3.

[0286] Tables 2.1 and 2.2 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00051 TABLE 2.1 Height growth data for TF0002Rp2 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TF0002rp2- 18 25 33 50 72 91 106 120 132 143 1B-1 TF0002rp2- 21 31 42 62 85 106 120 132 146 155 1B-2 TF0002rp2- 19 26 36 54 77 100 113 125 140 153 1B-3 TF0002rp2- 19 28 38 52 72 91 103 115 127 138 2A-1 TF0002rp2- 20 23 28 36 53 73 83 94 106 116 2A-2 TF0002rp2- 22 31 40 57 73 94 101 109 116 124 2A-3 TF0002rp2- 17 18 23 36 52 70 81 92 106 118 3B-1 TF0002rp2- 19 28 37 56 78 103 115 125 139 150 3B-2 TF0002rp2- 19 29 41 60 85 111 126 137 150 161 3B-3 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00052 TABLE 2.2 Diameter growth data for TF0002Rp2 Diameter (mm) Days in greenhouse 35 41 46 53 60 TF0002rp2-1B-1 4.6 5.4 6.3 8.0 8.2 TF0002rp2-1B-2 4.5 5.4 6.5 7.7 8.7 TF0002rp2-1B-3 4.9 5.9 6.4 7.2 8.0 TF0002rp2-2A-1 4.3 4.9 6.0 7.4 8.0 TF0002rp2-2A-2 4.1 5.3 6.3 6.5 7.6 TF0002rp2-2A-3 4.7 6.3 6.2 7.7 9.8 TF0002rp2-3B-1 3.4 4.4 5.1 6.9 7.1 TF0002rp2-3B-2 4.9 6.5 6.8 8.5 9.3 TF0002rp2-3B-3 4.7 6.3 6.9 8.5 8.7 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 NA 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0287] Results from growth analysis are specified in the overview table 2.3. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00053 TABLE 2.3 Overview table of growth effects of construct TF0002Rp2 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0002Rp2 1.29 1.03 1.36 1.10 1.27 1.05 1.38 1.09

Construction Group TF0003

[0288] Tables 2.4 and 2.5 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00054 TABLE 2.4 Height growth data for TF0003 Height (cm) Days in greenhouse 18 25 35 39 46 49 53 TF0003-1A 32 55 94 113 145 156 168 TF0003-1B 37 58 104 120 143 155 170 TF0003-2A 30 49 86 107 142 159 178 TF0003-3A 38 59 100 114 142 152 167 TF0003-3B 29 51 98 117 147 162 183 TF0003-4A 30 52 97 116 149 162 176 TF0003-4B 32 52 91 112 146 162 179 T89-01 33 57 108 128 161 173 192 T89-02 32 55 94 112 145 160 180 T89-03 31 53 95 114 147 160 173 T89-04 27 48 91 110 143 157 179 T89-05 26 43 86 107 141 155 171 T89-06 28 47 86 106 142 157 175 T89-07 34 56 101 120 153 168 184 T89-08 26 44 83 101 133 148 166 T89-09 29 51 88 106 141 154 171 T89-10 25 45 90 108 141 156 178 T89-11 26 46 80 96 128 143 166 T89-12 27 45 84 102 132 143 156 T89-13 29 50 96 115 149 163 185 T89-14 28 49 89 109 147 163 185 T89-15 25 43 81 99 134 146 161 T89-16 27 46 88 106 146 162 185 T89-17 31 52 90 107 142 158 179 T89-18 28 45 82 101 140 158 180 T89-19 27 42 82 101 132 146 163 T89-20 28 42 81 101 135 150 169 T89-21 26 36 67 76 100 113 128 T89-22 31 51 97 115 150 165 181 T89-23 29 49 83 101 132 144 163 T89-24 30 48 89 107 144 159 177 T89-25 26 45 89 103 136 152 175 T89-26 28 48 87 105 140 156 177 T89-27 28 46 86 103 138 153 170 T89-28 29 47 86 103 135 152 173 T89-29 26 43 81 101 139 156 179 T89-30 32 53 94 114 150 166 187 T89-31 30 48 85 103 134 148 169 T89-32 31 51 91 109 140 154 173 T89-33 31 52 94 113 151 167 186 T89-34 23 40 84 105 140 153 171 T89-35 29 48 88 106 137 150 165 T89-36 29 49 88 107 143 159 176 T89-37 32 49 91 105 141 154 168 T89-38 29 50 88 106 144 158 172 T89-39 31 48 89 106 139 152 172 T89-40 32 49 97 117 149 163 185 T89-41 24 46 86 104 138 155 176 T89-42 26 41 80 98 133 151 172 T89-43 29 48 92 110 145 163 188 T89-44 28 49 89 109 146 163 186 T89-45 28 48 83 97 119 129 145 T89-46 32 52 95 115 149 165 185 T89-47 26 42 83 101 133 147 166 T89-48 26 41 82 101 135 152 173 T89-49 33 52 103 123 153 166 180

TABLE-US-00055 TABLE 2.5 Diameter growth data for TF0003 Days in Diameter (mm) greenhouse 35 39 46 53 TF0003-1A 5.3 6.7 7.5 8.8 TF0003-1B 6.0 6.6 7.3 7.7 TF0003-2A 6.3 6.9 8.8 9.7 TF0003-3A 6.3 7.1 7.9 9.3 TF0003-3B 6.0 6.9 8.5 9.2 TF0003-4A 6.6 7.5 7.9 9.0 TF0003-4B 6.0 7.0 8.6 10.4 T89-01 7.2 8.0 9.6 10.7 T89-02 6.2 7.1 8.0 9.1 T89-03 6.3 7.3 8.3 9.3 T89-04 5.5 6.7 8.0 9.0 T89-05 5.3 6.5 7.4 8.3 T89-06 5.8 6.8 8.0 9.1 T89-07 6.3 7.3 9.1 10.9 T89-08 5.7 6.3 7.1 7.6 T89-09 6.0 6.6 8.1 9.1 T89-10 5.6 6.5 7.7 9.1 T89-11 5.8 6.6 7.7 8.6 T89-12 6.2 6.8 7.3 8.1 T89-13 5.7 6.6 7.7 8.8 T89-14 6.3 7.0 8.5 10.0 T89-15 5.2 6.0 6.9 7.6 T89-16 6.3 7.2 8.4 9.3 T89-17 5.9 6.8 8.0 9.1 T89-18 6.2 6.8 8.2 9.6 T89-19 6.1 6.8 7.9 9.1 T89-20 5.3 6.3 7.4 9.0 T89-21 3.5 4.2 5.0 9.2 T89-22 5.9 6.6 7.7 8.4 T89-23 6.1 7.0 8.4 9.7 T89-24 5.7 6.7 8.0 9.4 T89-25 5.9 6.8 8.1 8.7 T89-26 6.4 7.2 8.4 9.7 T89-27 5.7 6.9 8.8 10.1 T89-28 5.7 6.6 7.7 8.7 T89-29 5.1 6.4 7.7 9.1 T89-30 6.0 6.9 8.1 10.1 T89-31 5.8 6.7 8.2 9.3 T89-32 6.2 6.9 7.8 8.8 T89-33 6.3 7.1 8.5 10.2 T89-34 5.8 6.8 8.1 9.8 T89-35 6.1 7.2 7.7 8.9 T89-36 6.3 6.9 8.7 10.0 T89-37 5.9 7.0 8.4 9.2 T89-38 6.0 6.6 7.5 8.2 T89-39 7.1 7.6 8.4 9.7 T89-40 6.1 7.0 8.3 9.7 T89-41 5.5 6.4 6.9 7.9 T89-42 5.4 6.2 7.8 9.1 T89-43 6.0 6.9 8.3 9.5 T89-44 5.9 7.2 9.0 10.5 T89-45 5.0 5.7 6.2 7.8 T89-46 6.2 7.5 8.6 9.8 T89-47 5.3 6.1 7.8 8.8 T89-48 5.4 6.2 7.6 8.2 T89-49 7.0 8.2 9.4 10.3

[0289] Results from growth analysis are specified in the overview table 2.6. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00056 TABLE 2.6 Overview table of growth effects of construct TF0003 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0003 1.00 1.00 0.93 0.93 0.95 0.96 0.91 0.73

Construction Group TF0011

[0290] Tables 2.7 and 2.8 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00057 TABLE 2.7 Height growth data for TF0011 Height (cm) Days in greenhouse 19 25 32 39 48 54 56 TF0011-1A-1 17 28 48 73 107 133 141 TF0011-1A-2 23 35 58 80 101 114 118 TF0011-1B 17 27 45 66 94 111 117 TF0011-2A-1 23 32 56 81 110 125 131 TF0011-2A-2 18 33 55 76 105 127 134 TF0011-3A-1 19 31 53 75 105 125 131 TF0011-3A-2 17 27 47 67 95 116 121 TF0011-3B-1 17 29 47 72 100 121 127 TF0011-3B-2 14 24 45 67 101 123 130 TF0011-4A 19 33 57 81 114 135 143 T89-01 18 28 49 70 100 126 133 T89-02 19 29 51 76 105 128 136 T89-03 11 18 36 47 76 93 98 T89-04 16 25 48 74 102 126 133 T89-05 17 29 49 73 106 128 135 T89-06 16 28 51 74 105 127 132 T89-07 19 28 51 73 104 125 136 T89-08 19 31 52 77 110 130 137 T89-09 17 26 44 67 93 114 122 T89-10 16 25 44 63 89 108 115 T89-11 20 31 47 68 87 114 118 T89-12 17 28 48 70 101 117 124 T89-13 18 30 52 73 105 125 130 T89-14 19 29 44 67 96 116 123 T89-15 17 29 49 72 102 124 131 T89-16 18 29 50 74 108 129 135 T89-17 14 23 43 65 93 116 123 T89-18 15 26 48 72 103 128 136 T89-19 11 19 38 63 93 115 122 T89-20 19 30 52 75 104 126 133 T89-21 17 28 48 72 104 128 135 T89-22 19 29 49 73 105 123 129 T89-23 21 30 54 79 112 135 138 T89-24 19 24 37 54 77 100 103 T89-25 15 27 46 69 98 117 123 T89-26 19 29 50 73 103 127 135 T89-27 15 27 47 70 106 129 135 T89-28 20 33 55 80 109 130 137 T89-29 24 38 62 88 122 143 151 T89-30 21 33 56 78 113 132 138 T89-31 21 31 52 76 105 126 133 T89-32 18 29 50 73 105 129 138 T89-33 23 37 61 89 121 143 150 T89-34 19 30 52 78 108 130 138 T89-35 19 31 55 79 111 134 142 T89-36 18 29 52 75 105 127 134 T89-37 22 32 52 73 103 124 130 T89-38 18 29 51 74 105 128 134 T89-39 21 31 51 72 102 119 124 T89-40 20 32 51 74 99 121 128 T89-41 19 29 50 73 108 130 135 T89-42 15 26 46 68 95 113 119 T89-43 16 28 48 67 93 113 119 T89-44 21 31 50 70 97 116 122 T89-45 16 26 48 72 101 123 131 T89-46 21 29 50 75 106 129 138 T89-47 9 16 34 55 82 106 114 T89-48 5 8 17 24 42 52 56 T89-49 20 30 50 71 96 117 125 T89-50 16 28 50 74 105 128 136 T89-51 19 29 51 75 107 128 136 T89-52 20 29 50 75 104 124 131 T89-53 21 32 50 72 101 118 125 T89-54 21 32 55 81 112 133 142 T89-55 21 32 56 83 112 133 141

TABLE-US-00058 TABLE 2.8 Diameter growth data for TF0011 Diameter (mm) Days in greenhouse 39 48 54 56 TF0011-1A-1 5.7 6.9 7.6 7.9 TF0011-1A-2 5.0 5.6 5.6 6.0 TF0011-1B 5.2 7.1 7.7 7.6 TF0011-2A-1 5.8 7.9 8.1 8.0 TF0011-2A-2 6.2 7.9 9.2 9.3 TF0011-3A-1 6.0 8.4 8.4 8.8 TF0011-3A-2 6.0 7.5 8.0 8.5 TF0011-3B-1 6.3 8.5 9.5 9.7 TF0011-3B-2 7.7 8.4 9.3 9.5 TF0011-4A 6.7 8.1 9.2 9.6 T89-01 6.6 8.4 8.5 9.2 T89-02 6.8 8.3 9.4 10.1 T89-03 4.6 4.9 6.1 6.1 T89-04 6.9 8.7 10.0 9.4 T89-05 5.6 7.6 8.2 8.8 T89-06 6.9 8.2 9.6 9.3 T89-07 6.6 7.9 9.5 9.5 T89-08 6.9 8.9 9.6 9.8 T89-09 6.1 7.6 8.6 8.7 T89-10 5.5 7.5 8.6 8.6 T89-11 7.4 8.4 9.3 8.7 T89-12 5.9 7.4 8.7 9.5 T89-13 6.2 7.7 9.2 9.5 T89-14 5.9 7.8 9.2 8.7 T89-15 6.8 8.1 9.4 9.7 T89-16 7.3 9.2 10.6 10.3 T89-17 6.4 8.0 8.9 9.1 T89-18 7.0 7.5 8.1 8.8 T89-19 5.6 7.4 8.6 8.9 T89-20 6.5 8.3 8.6 9.5 T89-21 6.2 8.1 8.0 8.7 T89-22 6.2 8.5 9.3 10.1 T89-23 6.2 8.1 9.2 9.5 T89-24 3.1 4.6 5.0 4.5 T89-25 5.9 7.7 8.5 8.9 T89-26 6.2 7.8 8.6 9.5 T89-27 6.1 7.6 8.2 8.6 T89-28 6.9 9.0 10.7 10.5 T89-29 7.8 8.4 10.3 10.2 T89-30 6.4 8.5 9.4 9.9 T89-31 6.8 8.6 8.8 9.5 T89-32 6.7 8.4 10.2 10.9 T89-33 7.1 8.7 9.4 11.4 T89-34 7.1 8.5 11.1 10.9 T89-35 6.7 9.0 8.9 11.4 T89-36 6.2 8.0 8.5 8.5 T89-37 5.6 7.1 7.8 8.7 T89-38 5.8 7.8 8.8 8.7 T89-39 6.2 8.1 9.2 9.3 T89-40 6.2 7.9 9.6 9.5 T89-41 5.9 8.2 8.9 9.5 T89-42 6.8 8.4 9.1 10.4 T89-43 6.7 8.5 9.7 9.3 T89-44 7.1 9.1 9.2 9.4 T89-45 6.6 7.8 9.2 9.2 T89-46 6.8 9.3 10.2 10.0 T89-47 5.3 7.0 7.9 8.4 T89-48 2.3 3.0 3.2 3.5 T89-49 7.5 8.7 9.1 9.8 T89-50 6.8 8.6 9.3 8.9 T89-51 7.2 8.3 9.2 9.1 T89-52 7.5 8.8 9.4 9.7 T89-53 7.3 9.3 9.5 10.2 T89-54 7.0 9.0 9.2 9.6 T89-55 7.0 8.9 9.2 9.8

[0291] Results from growth analysis are specified in the overview table 2.9. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00059 TABLE 2.9 Overview table of growth effects of construct TF0011 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0011 1.00 0.92 1.00 0.83 0.95 0.85 1.04 0.77

Construction Group TF0013rp2

[0292] Tables 2.10 and 2.11 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00060 TABLE 2.10 Height growth data for TF0013rp2 Height (cm) Days in greenhouse 15 19 26 33 37 40 47 51 54 TF0013rp2-1A-1 20 25 39 48 59 68 89 100 111 TF0013rp2-1A-2 18 22 34 52 64 74 101 115 131 TF0013rp2-1A-3 21 25 38 54 67 75 104 116 133 TF0013rp2-3BA-1 21 26 39 57 73 84 113 125 137 TF0013rp2-3BA-2 20 23 34 52 65 75 101 113 128 TF0013rp2-3BA-3 20 24 36 55 71 83 115 131 145 T89-20 21 26 39 58 73 82 111 130 134 T89-21 21 24 34 51 64 72 97 103 125 T89-22 19 23 32 48 60 70 96 111 123 T89-23 21 24 34 51 63 70 94 112 122 T89-24 19 22 30 46 59 68 90 103 115 T89-25 22 30 39 57 72 81 106 112 130 T89-26 21 24 35 48 60 70 98 111 130 T89-27 21 24 34 48 60 70 93 106 120 T89-28 20 22 32 48 60 70 93 105 116

TABLE-US-00061 TABLE 2.11 Diameter growth data for TF0013rp2 Diameter (mm) Days in greenhouse 33 40 47 54 TF0013rp2-1A-1 3.8 5.0 6.3 6.8 TF0013rp2-1A-2 5.4 5.7 7.3 8.1 TF0013rp2-1A-3 4.4 5.4 6.6 7.6 TF0013rp2-3BA-1 5.0 6.3 7.1 8.5 TF0013rp2-3BA-2 4.2 5.4 7.0 8.5 TF0013rp2-3BA-3 5.0 6.5 7.3 8.1 T89-20 4.8 6.2 6.3 6.9 T89-21 3.8 5.5 6.0 6.9 T89-22 4.5 6.2 6.6 7.5 T89-21 3.8 5.5 6.0 6.9 T89-22 4.5 6.2 6.6 7.5 T89-23 3.8 4.8 5.6 6.2 T89-24 3.8 4.9 5.8 7.2 T89-25 4.8 5.9 7.4 8.9 T89-26 4.4 6.2 7.0 7.8 T89-27 4.9 6.4 7.3 8.3 T89-28 4.5 4.8 5.8 7.2

[0293] Results from growth analysis are specified in the overview table 2.12. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00062 TABLE 2.12 Overview table of growth effects of construct TF0013rp2 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0013rp2 1.04 1.03 1.08 1.06 1.08 0.91 1.06 0.96

Construction Group TF0045

[0294] This construct induces increased growth. The final height is 6% higher comparing the average of the construction group and wild type control group. The final height is 11% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 9% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 12% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 10% higher comparing the average of the construction group and wild type control group. The final diameter is 9% higher comparing the largest individuals of the construction group and wild type control group. The TF0045 construction group meets growth difference selection criterion (1) as shown in table 2.15.

[0295] Tables 2.13 and 2.14 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00063 TABLE 2.13 Height growth data for TF0045 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0045-1A-1 25 39 53 66 79 87 106 125 128 TF0045-1A-2 15 25 34 44 57 66 89 114 121 TF0045-1A-3 18 34 45 55 68 79 104 133 141 TF0045-1B-1 24 43 57 68 84 95 126 150 156 TF0045-1B-2 24 43 57 70 85 95 120 147 154 TF0045-1B-3 25 30 34 43 54 64 84 107 115 TF0045-2B-1 21 33 45 53 64 76 103 128 136 TF0045-2B-3 19 32 42 51 64 72 96 120 128 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00064 TABLE 2.14 Diameter growth data for TF0045 Days in Diameter (mm) greenhouse 32 39 46 53 55 TF0045-1A-1 5.8 6.5 6.9 7.5 7.6 TF0045-1A-2 4.3 6.8 8.4 8.7 9.2 TF0045-1A-3 5.6 6.7 7.9 9.2 9.2 TF0045-1B-1 5.9 7.1 8.8 9.7 10.0 TF0045-1B-2 6.8 7.8 8.4 10.1 9.9 TF0045-1B-3 4.3 4.4 4.6 5.9 5.9 TF0045-2B-1 5.3 7.1 7.4 8.5 8.8 TF0045-2B-3 5.4 6.2 8.0 8.9 8.8 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0296] Results from growth analysis are specified in the overview table 2.15. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00065 TABLE 2.15 Overview table of growth effects of construct TF0045 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0045 1.06 1.10 1.09 0.98 1.11 1.09 1.12 0.94

Construction Group TF0052Rp1

[0297] Tables 2.16 and 2.17 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00066 TABLE 2.16 Height growth data for TF0052Rp1 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TF0052rp1- 21 29 38 53 74 90 103 115 127 138 2A-1 TF0052rp1- 19 28 37 52 68 83 97 105 116 126 2A-2 TF0052rp1- 15 23 33 50 69 85 97 108 117 129 2A-3 TF0052rp1- 18 26 32 47 59 75 82 91 100 109 2B-1 TF0052rp1- 18 23 27 35 49 67 75 84 94 101 2B-2 TF0052rp1- 18 21 28 38 50 63 68 75 83 89 2B-3 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00067 TABLE 2.17 Diameter growth data for TF0052Rp1 Diameter (mm) Days in greenhouse 35 41 46 53 60 TF0052rp1-2A-1 5.4 6.5 7.2 8.6 9.4 TF0052rp1-2A-2 4.9 6.6 6.9 7.3 8.3 TF0052rp1-2A-3 5.3 6.7 7.5 8.4 9.5 TF0052rp1-2B-1 5.0 6.2 6.9 9.0 8.0 TF0052rp1-2B-2 4.9 5.9 6.6 6.8 8.1 TF0052rp1-2B-3 3.3 4.2 4.4 5.1 5.7 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 N/A 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0298] Results from growth analysis are specified in the overview table 2.18. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00068 TABLE 2.18 Overview table of growth effects of construct TF0052Rp1 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0052Rp1 1.06 1.00 1.04 0.91 1.09 1.02 1.06 0.86

Construction Group TF0076Rp2

[0299] This construct induces increased growth. The final height is 13% higher comparing the average of the construction group and wild type control group. The final height is 13% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 13% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 18% higher comparing the largest individuals of the construction group and wild type control group. The TF0076Rp2 construction group meets the more stringent level of growth difference selection criterion (1) as shown in table 2.21.

[0300] Tables 2.19 and 2.20 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00069 TABLE 2.19 Height growth data for TF0076Rp2 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TF0076rp2- 15 22 29 43 59 72 84 93 103 111 3BB-1 TF0076rp2- 20 28 39 53 70 85 95 104 115 120 3BB-2 TF0076rp2- 17 25 33 48 67 81 92 101 112 122 3BB-3 TF0076rp2- 15 26 35 55 76 97 111 122 134 143 4B-1 TF0076rp2- 19 28 37 53 72 89 103 111 124 135 4B-2 TF0076rp2- 20 28 40 53 74 93 104 114 124 134 4B-3 TF0076rp2- 18 24 30 39 49 65 74 84 96 103 5BA-1 TF0076rp2- 17 26 36 49 63 82 92 100 112 121 5BA-2 TF0076rp2- 16 24 31 46 65 84 93 103 116 126 5BA-3 TF0076rp2- 17 24 30 45 61 76 86 97 109 119 5BB-1 TF0076rp2- 15 22 29 42 58 73 86 95 106 118 5BB-2 TF0076rp2- 20 27 36 50 69 N/A 94 100 109 118 5BB-3 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00070 TABLE 2.20 Diameter growth data for TF0076Rp2 Diameter (mm) Days in greenhouse 35 41 46 53 60 TF0076rp2-3BB-1 4.1 5.3 6.4 8.3 8.2 TF0076rp2-3BB-2 4.6 5.5 6.9 7.4 8.4 TF0076rp2-3BB-3 5.3 6.6 7.3 8.5 9.1 TF0076rp2-4B-1 4.4 5.8 6.8 7.5 8.0 TF0076rp2-4B-2 4.4 5.7 6.5 7.0 7.9 TF0076rp2-4B-3 4.9 6.2 7.5 8.2 9.1 TF0076rp2-5BA-1 4.7 5.5 6.5 6.8 7.3 TF0076rp2-5BA-2 4.4 6.2 6.4 7.4 8.1 TF0076rp2-5BA-3 4.4 5.5 6.8 7.8 8.6 TF0076rp2-5BB-1 4.5 5.8 6.1 7.3 8.0 TF0076rp2-5BB-2 4.1 5.9 6.5 7.1 8.2 TF0076rp2-5BB-3 4.1 6.1 6.1 6.4 8.1 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 N/A 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0301] Results from growth analysis are specified in the overview table 2.21. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00071 TABLE 2.21 Overview table of growth effects of construct TF0076Rp2 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0076Rp2 1.13 1.01 1.13 1.01 1.13 0.97 1.18 0.87

Construction Group TF0096

[0302] This construct induces increased growth. The final height is 11% higher comparing the average of the construction group and wild type control group. The final height is 8% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 18% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 14% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 15% higher comparing the average of the construction group and wild type control group. The final diameter is 8% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 27% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 14% higher comparing the largest individuals of the construction group and wild type control group. The TF0096 construction group meets the more stringent level of growth difference selection criterion (3) and the less stringent level of growth difference selection criteria (1) and (2) as shown in table 2.24.

[0303] Tables 2.22 and 2.23 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00072 TABLE 2.22 Height growth data for TF0096 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0096-2A 14 27 41 51 68 80 109 138 148 TF0096-2B 13 24 36 47 62 72 101 126 131 TF0096-3A 12 26 38 50 65 77 106 133 141 TF0096-3B 16 29 39 50 62 72 101 127 134 TF0096-4A 24 42 54 67 82 93 120 N/A 151 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00073 TABLE 2.23 Diameter growth data for TF0096 Days in Diameter (mm) greenhouse 32 39 46 53 55 TF0096-2A 4.8 6.0 7.3 8.4 8.6 TF0096-2B 4.4 6.2 8.2 9.2 9.9 TF0096-3A 5.5 6.8 7.4 9.2 9.2 TF0096-3B 3.9 5.5 7.3 8.2 8.4 TF0096-4A 5.5 6.9 7.6 9.2 9.1 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0304] Results from growth analysis are specified in the overview table 2.24. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00074 TABLE 2.24 Overview table of growth effects of construct TF0096 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0096 1.11 1.15 1.18 1.27 1.08 1.08 1.14 1.14

Construction Group TF0097Rp1

[0305] This construct induces increased growth. The final height is 33% higher comparing the average of the construction group and wild type control group. The final height is 43% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 32% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 41% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 11% higher comparing the average of the construction group and wild type control group. The final diameter is 13% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 20% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 26% higher comparing the largest individuals of the construction group and wild type control group. The TF0097Rp1 construction group meets the more stringent level of growth difference selection criteria (1), (2), (3) and (4) as shown in table 2.27.

[0306] Tables 2.25 and 2.26 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00075 TABLE 2.25 Height growth data for TF0097Rp1 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TF0097rp1- 25 32 41 61 86 107 123 137 153 166 1A-1 TF0097rp1- 29 40 52 69 91 113 129 140 155 170 1A-2 TF0097rp1- 27 35 40 57 81 99 115 126 140 151 1A-3 TF0097rp1- 23 32 43 61 75 92 102 111 123 132 2A-1 TF0097rp1- 19 31 41 57 75 92 102 111 122 132 2A-2 TF0097rp1- 19 29 38 52 68 86 94 103 112 121 2A-3 TF0097rp1- 26 33 43 61 80 93 102 110 118 124 2B-1 TF0097rp1- 26 34 45 62 83 102 114 125 138 147 2B-2 TF0097rp1- 22 32 44 63 86 101 117 127 144 155 2B-3 TF0097rp1- 27 40 49 72 96 115 137 150 165 181 3A-1 TF0097rp1- 13 23 34 50 73 93 108 119 132 143 3A-2 TF0097rp1- 19 31 44 63 88 109 125 137 151 162 3A-3 TF0097rp1- 24 35 42 48 62 81 91 99 108 116 4A-1 TF0097rp1- 14 22 28 39 55 80 90 103 115 126 4A-2 TF0097rp1- 19 25 29 46 67 80 99 109 125 136 4A-3 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00076 TABLE 2.26 Diameter growth data for TF0097Rp1 Days in Diameter (mm) greenhouse 35 41 46 53 60 TF0097rp1-1A-1 4.3 5.3 6.2 8.0 9.5 TF0097rp1-1A-2 4.5 5.3 6.0 7.4 8.8 TF0097rp1-1A-3 4.4 5.2 5.3 6.4 8.1 TF0097rp1-2A-1 5.7 6.9 7.8 9.6 9.9 TF0097rp1-2A-2 5.3 6.7 7.5 7.9 9.3 TF0097rp1-2A-3 5.1 6.5 7.5 7.8 9.2 TF0097rp1-2B-1 4.6 5.5 6.2 6.8 7.5 TF0097rp1-2B-2 4.9 6.0 7.1 7.6 10.2 TF0097rp1-2B-3 5.3 7.7 8.2 9.9 10.6 TF0097rp1-3A-1 5.7 7.0 7.7 9.4 9.8 TF0097rp1-3A-2 4.7 6.2 7.0 7.3 9.5 TF0097rp1-3A-3 5.0 6.5 7.1 9.0 10.2 TF0097rp1-4A-1 4.4 5.0 6.0 7.3 6.7 TF0097rp1-4A-2 4.1 5.4 5.9 6.3 7.2 TF0097rp1-4A-3 3.7 4.9 6.0 8.6 9.4 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 N/A 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0307] Results from growth analysis are specified in the overview table 2.27. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00077 TABLE 2.27 Overview table of growth effects of construct TF0097Rp1 Average Maximum Maximum Average of Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0097Rp1 1.33 1.11 1.32 1.20 1.43 1.13 1.41 1.26

Construction Group TF0104

[0308] This construct induces increased growth. The final height is 15% higher comparing the average of the construction group and wild type control group. The final height is 12% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 16% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 14% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 23% higher comparing the average of the construction group and wild type control group. The final diameter is 20% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 20% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 10% higher comparing the largest individuals of the construction group and wild type control group. The TF0104 construction group meets the more stringent level of growth difference selection criteria (1), (2) and (3) and the less stringent level of growth difference selection criterion (4) as shown in table 2.30.

[0309] Tables 2.28 and 2.29 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00078 TABLE 2.28 Height growth data for TF0104 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0104-1A 20 35 48 60 77 90 117 149 157 TF0104-1B 23 37 50 63 78 90 120 148 155 TF0104-2A 21 37 50 62 75 85 114 140 147 TF0104-3A 20 37 50 61 74 85 102 115 119 TF0104-3B 20 35 50 62 78 89 118 145 152 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00079 TABLE 2.29 Diameter growth data for TF0104 Days in Diameter (mm) greenhouse 32 39 46 53 55 TF0104-1A 6.0 7.2 8.7 10.4 11.0 TF0104-1B 5.7 7.9 8.8 10.0 10.6 TF0104-2A 5.7 8.0 9.0 10.4 10.2 TF0104-3A 5.3 6.3 6.7 7.5 7.1 TF0104-3B 5.7 6.9 8.2 9.8 9.5 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0310] Results from growth analysis are specified in the overview table 2.30. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00080 TABLE 2.30 Overview table of growth effects of construct TF0104 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0104 1.15 1.23 1.16 1.20 1.12 1.20 1.14 1.10

Construction Group TF0109Rp1

[0311] This construct induces increased growth. The final height is 22% higher comparing the average of the construction group and wild type control group. The final height is 32% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 26% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 40% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 15% higher comparing the average of the construction group and wild type control group. The final diameter is 14% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 25% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 27% higher comparing the largest individuals of the construction group and wild type control group. The TF0109Rp1 construction group meets the more stringent level of growth difference selection criteria (1), (2), (3) and (4) as shown in table 2.33.

[0312] Tables 2.31 and 2.32 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00081 TABLE 2.31 Height growth data for TF0109Rp1 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TF0109rp1- 21 31 39 55 73 89 100 109 122 130 2A-1 TF0109rp1- 18 25 33 47 65 79 91 101 111 120 2A-2 TF0109rp1- 17 23 31 47 63 77 87 96 109 118 2A-3 TF0109rp1- 19 26 35 54 78 103 117 129 141 149 2B-1 TF0109rp1- 21 31 39 57 82 105 124 136 151 168 2B-2 TF0109rp1- 16 27 40 51 80 102 117 122 137 150 2B-3 TF0109rp1- 18 29 39 59 77 94 104 114 126 136 3B-1 TF0109rp1- 18 28 37 54 70 88 98 108 118 125 3B-2 TF0109rp1- 17 28 37 51 65 87 94 103 115 120 3B-3 TF0109rp1- 16 25 32 46 64 79 94 104 116 126 4A-1 TF0109rp1- 17 24 32 46 65 83 94 106 118 126 4A-2 TF0109rp1- 17 19 26 38 57 73 85 96 110 121 4A-3 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00082 TABLE 2.32 Diameter growth data for TF0109Rp1 Days in Diameter (mm) greenhouse 35 41 46 53 60 TF0109rp1-2A-1 5.7 7.3 8.1 9.1 9.9 TF0109rp1-2A-2 4.7 5.7 6.7 7.8 8.8 TF0109rp1-2A-3 4.6 6.2 6.5 8.1 9.3 TF0109rp1-2B-1 3.8 5.2 5.6 6.7 8.0 TF0109rp1-2B-2 4.3 5.4 6.3 6.9 7.9 TF0109rp1-2B-3 5.1 6.2 7.3 8.2 9.3 TF0109rp1-3B-1 5.1 7.0 7.3 7.7 9.1 TF0109rp1-3B-2 5.3 6.1 7.5 7.7 8.7 TF0109rp1-3B-3 4.9 5.3 6.7 8.1 9.3 TF0109rp1-4A-1 5.2 6.5 7.5 8.7 10.5 TF0109rp1-4A-2 5.4 6.5 7.8 9.2 10.7 TF0109rp1-4A-3 4.8 6.2 7.3 9.1 10.7 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 N/A 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0313] Results from growth analysis are specified in the overview table 2.33. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00083 TABLE 2.33 Overview table of growth effects of construct TF0109Rp1 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0109Rp1 1.22 1.15 1.26 1.25 1.32 1.14 1.40 1.27

Construction Group TF0116

[0314] Tables 2.34 and 2.35 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00084 TABLE 2.34 Height growth data for TF0116 Height (cm) Days in greenhouse 19 28 34 40 44 47 51 54 61 65 TF0116-1B N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A TF0116-2A 18 37 51 67 77 86 98 106 123 133 TF0116-2B-1 18 34 50 65 76 84 98 106 126 139 TF0116-2B-2 17 31 48 61 75 82 93 101 120 133 TF0116-4A 16 33 53 68 81 91 103 113 133 142 TF0116-5B 21 40 56 70 78 88 98 105 124 136 TF0116-6A 21 36 55 71 83 90 101 110 131 143 TF0116-6B 17 29 45 59 70 80 92 102 125 138 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

TABLE-US-00085 TABLE 2.35 Diameter growth data for TF0116 Diameter (mm) Days in greenhouse 28 34 40 44 47 51 54 61 65 TF0116-1B N/A N/A N/A N/A N/A N/A N/A N/A N/A TF0116-2A 3.7 5.9 7.3 8.2 8.6 9.2 10.0 10.5 10.9 TF0116-2B-1 3.5 5.3 6.2 6.9 8.0 8.4 9.0 10.0 10.2 TF0116-2B-2 3.4 5.0 5.8 6.6 7.3 8.4 8.4 10.7 10.3 TF0116-4A 3.8 5.8 6.5 7.2 8.0 8.7 9.4 10.2 10.9 TF0116-5B 3.9 5.0 6.3 6.9 7.6 8.2 8.4 10.3 10.4 TF0116-6A 3.9 4.9 6.7 7.5 7.9 9.0 9.4 10.7 11.1 TF0116-6B 3.7 4.7 5.9 7.2 7.7 9.2 9.7 10.5 10.8 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 N/A 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[0315] Results from growth analysis are specified in the overview table 2.36. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00086 TABLE 2.36 Overview table of growth effects of construct TF0116 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0116 1.10 1.12 1.03 1.13 1.03 0.90 0.99 0.90

Construction Group TF0132.2nd

[0316] This construct induces increased growth. The final height is 27% higher comparing the average of the construction group and wild type control group. The final height is 32% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 38% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 41% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 12% higher comparing the average of the construction group and wild type control group. The final diameter is 9% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 15% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 8% higher comparing the largest individuals of the construction group and wild type control group. The TF0132.2nd construction group meets the more stringent level of growth difference selection criteria (1), (3) and (4) and the less stringent level of growth difference selection criterion (2) as shown in table 2.39.

[0317] Tables 2.37 and 2.38 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00087 TABLE 2.37 Height growth data for TF0132.2nd Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0132.2nd- 17 30 40 51 66 78 103 132 139 1A TF0132.2nd- 19 34 48 63 82 96 132 168 178 1B TF0132.2nd- 19 32 40 52 67 77 93 108 112 2A TF0132.2nd- 22 41 57 73 93 106 138 175 185 4B TF0132.2nd- 19 33 47 62 73 84 110 143 153 5A TF0132.2nd- 19 35 49 63 84 101 136 168 177 5B TF0132.2nd- 21 37 51 64 83 98 133 170 180 6B TF0132.2nd- 20 34 47 59 77 92 125 160 175 7A T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00088 TABLE 2.38 Diameter growth data for TF0132.2nd Days in Diameter (mm) greenhouse 32 39 46 53 55 TF0132.2nd-1A 4.7 6.1 7.5 8.8 9.3 TF0132.2nd-1B 5.3 6.3 7.6 10.2 10.0 TF0132.2nd-2A 5.0 6.0 6.5 7.2 6.7 TF0132.2nd-4B 5.8 6.5 7.5 9.6 9.6 TF0132.2nd-5A 5.1 6.0 6.4 7.7 8.0 TF0132.2nd-5B 5.2 6.4 7.2 8.4 8.3 TF0132.2nd-6B 4.9 6.3 7.6 9.1 9.3 TF0132.2nd-7A 4.9 6.7 8.0 9.1 9.5 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0318] Results from growth analysis are specified in the overview table 2.39. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00089 TABLE 2.39 Overview table of growth effects of construct TF0132.2nd Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0132.2nd 1.27 1.12 1.38 1.15 1.32 1.09 1.41 1.08

Construction Group TF0132rp1

[0319] This construct induces increased growth. The final height is 29% higher comparing the average of the construction group and wild type control group. The final height is 28% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 31% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 23% higher comparing the largest individuals of the construction group and wild type control group.

[0320] The TF0132rp1 construction group meets the more stringent level of growth difference selection criteria (1), (3) and (4) as shown in table 2.42.

[0321] Tables 2.40 and 2.41 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00090 TABLE 2.40 Height growth data for TF0132rp1 Height (cm) Days in greenhouse 15 19 26 33 37 40 47 51 54 TF0132rp1-1B-1 25 30 48 70 88 103 137 153 170 TF0132rp1-1B-2 23 26 38 63 80 96 133 151 168 TF0132rp1-1B-3 23 27 42 66 86 98 131 151 167 TF0132rp1-3BB-1 21 25 36 58 76 90 124 140 157 TF0132rp1-3BB-2 21 28 46 68 88 101 135 147 171 TF0132rp1-3BB-3 18 22 38 66 81 94 129 144 161 TF0132rp1-4AC-1 22 27 42 65 83 95 127 142 159 TF0132rp1-4AC-2 20 25 41 65 81 94 125 140 156 TF0132rp1-4AC-3 21 24 38 61 77 88 122 139 154 TF0132rp1-4B-1 26 30 45 70 87 100 133 144 162 TF0132rp1-4B-2 20 26 43 64 77 90 121 135 152 TF0132rp1-4B-3 20 24 41 67 85 100 132 147 163 TF0132rp1-6B-1 23 26 41 65 83 95 127 141 157 TF0132rp1-6B-2 23 28 41 64 82 96 132 152 165 TF0132rp1-6B-3 20 26 45 73 93 107 140 156 169 T89-20 21 26 39 58 73 82 111 130 134 T89-21 21 24 34 51 64 72 97 103 125 T89-22 19 23 32 48 60 70 96 111 123 T89-23 21 24 34 51 63 70 94 112 122 T89-24 19 22 30 46 59 68 90 103 115 T89-25 22 30 39 57 72 81 106 112 130 T89-26 21 24 35 48 60 70 98 111 130 T89-27 21 24 34 48 60 70 93 106 120 T89-28 20 22 32 48 60 70 93 105 116

TABLE-US-00091 TABLE 2.41 Diameter growth data for TF0132rp1 Days in Diameter (mm) greenhouse 33 40 47 54 TF0132rp1-1B-1 5.1 6.3 7.4 9.1 TF0132rp1-1B-2 4.8 5.8 6.9 8.1 TF0132rp1-1B-3 4.2 5.8 7.0 7.6 TF0132rp1-3BB-1 4.0 5.5 6.7 7.4 TF0132rp1-3BB-2 5.1 6.1 6.8 8.2 TF0132rp1-3BB-3 4.9 5.7 6.8 8.4 TF0132rp1-4AC-1 4.3 6.2 8.0 9.1 TF0132rp1-4AC-2 5.0 6.6 8.5 9.4 TF0132rp1-4AC-3 4.8 6.5 7.5 8.5 TF0132rp1-4B-1 4.8 5.1 6.5 7.9 TF0132rp1-4B-2 4.8 5.3 6.3 7.2 TF0132rp1-4B-3 4.5 5.6 7.7 8.4 TF0132rp1-6B-1 4.2 5.3 6.6 6.9 TF0132rp1-6B-2 4.5 5.8 7.6 8.2 TF0132rp1-6B-3 6.5 6.7 7.3 8.4 T89-20 4.8 6.2 6.3 6.9 T89-21 3.8 5.5 6.0 6.9 T89-22 4.5 6.2 6.6 7.5 T89-23 3.8 4.8 5.6 6.2 T89-24 3.8 4.9 5.8 7.2 T89-25 4.8 5.9 7.4 8.9 T89-26 4.4 6.2 7.0 7.8 T89-27 4.9 6.4 7.3 8.3 T89-28 4.5 4.8 5.8 7.2

[0322] Results from growth analysis are specified in the overview table 2.42. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00092 TABLE 2.42 Overview table of growth effects of construct TF0132rp1 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0132rp1 1.29 1.07 1.31 1.10 1.28 1.01 1.23 1.02

Construction Group TF0146

[0323] This construct induces increased growth. The final height is 13% higher comparing the average of the construction group and wild type control group. The final height is 16% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 18% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 25% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 8% higher comparing the average of the construction group and wild type control group. The final diameter is 8% higher comparing the largest individuals of the construction group and wild type control group. The TF0146 construction group meets the more stringent level of growth difference selection criteria (1) and (4) as shown in table 2.45.

[0324] Tables 2.43 and 2.44 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00093 TABLE 2.43 Height growth data for TF0146 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0146-1A 25 43 51 60 80 94 125 152 158 TF0146-1B 16 32 42 55 68 82 113 140 148 TF0146-2A 18 31 41 51 64 74 98 124 132 TF0146-2B 22 42 55 64 81 95 125 156 162 TF0146-3A 24 38 51 63 77 89 116 139 144 TF0146-3B 18 30 38 49 64 74 95 114 122 TF0146-4A 20 34 47 58 72 84 107 124 132 TF0146-4B 24 39 52 63 79 91 120 149 158 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00094 TABLE 2.44 Diameter growth data for TF0146 Days in Diameter (mm) greenhouse 32 39 46 53 55 TF0146-1A 5.4 6.2 7.2 6.9 7.5 TF0146-1B 4.2 5.7 7.0 8.3 8.6 TF0146-2A 6.1 6.4 7.4 8.7 9.4 TF0146-2B 5.5 7.0 8.5 9.9 9.9 TF0146-3A 6.0 7.1 8.1 8.3 8.3 TF0146-3B 4.2 5.6 6.8 8.5 8.4 TF0146-4A 4.8 5.1 6.9 7.2 7.2 TF0146-4B 5.5 8.8 8.5 10.0 9.1 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0325] Results from growth analysis are specified in the overview table 2.45. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00095 TABLE 2.45 Overview table of growth effects of construct TF0146 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0146 1.13 1.08 1.18 0.99 1.16 1.08 1.25 0.95

Construction Group TF0173

[0326] This construct induces increased growth. The diameter growth rate is 19% higher comparing the average of the construction group and wild type control group. The TF0173 construction group meets growth difference selection criterion (3) as shown in table 2.48.

[0327] Tables 2.46 and 2.47 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00096 TABLE 2.46 Height growth data for TF0173 Height (cm) Days in greenhouse 22 27 31 34 38 41 45 48 52 56 TF0173-3A-1 28 39 51 60 73 81 93 104 120 134 TF0173-3A-2 24 34 44 53 65 74 87 98 111 123 TF0173-3B-1 30 42 57 67 81 94 108 120 136 155 TF0173-3B-2 26 37 50 62 76 88 103 114 131 147 TF0173-4A-1 26 37 51 60 76 87 101 112 125 141 TF0173-4A-2 28 40 54 64 77 88 102 111 125 142 TF0173-4B-1 30 41 57 65 80 91 105 115 N/A 136 TF0173-4B-2 30 38 49 58 71 82 95 105 116 140 T89-01 27 37 47 58 69 79 93 100 114 129 T89-02 28 38 48 57 69 75 84 91 97 104 T89-03 26 38 49 59 69 82 94 106 122 139 T89-04 27 37 49 58 70 82 99 110 124 140 T89-05 29 40 52 60 74 83 96 106 120 134 T89-06 29 39 51 59 70 85 97 105 118 133 T89-07 24 32 43 52 61 71 81 90 96 102 T89-08 27 35 45 54 67 76 88 99 114 129 T89-09 30 40 53 64 75 86 99 110 123 136 T89-10 26 37 48 59 73 80 92 101 115 129 T89-12 32 43 56 68 80 93 108 117 131 144 T89-13 29 41 50 61 72 82 93 104 118 136 T89-14 28 36 47 58 69 77 89 100 115 132 T89-15 24 38 48 57 68 78 93 106 120 135 T89-16 29 40 53 63 74 85 N/A 109 122 137 T89-18 30 40 52 61 73 81 93 104 115 124 T89-19 30 43 56 67 80 92 108 117 132 149 T89-20 31 42 56 65 78 89 102 113 129 145 T89-21 31 42 55 64 78 89 102 116 132 147 T89-23 32 41 52 62 74 85 97 107 120 135 T89-24 25 36 47 55 69 78 92 101 113 128 T89-25 30 40 52 61 73 84 101 112 125 139 T89-26 28 40 50 60 72 85 97 109 122 136 T89-27 27 36 46 53 65 74 85 96 109 123 T89-31 30 41 51 61 73 84 98 109 123 138 T89-32 28 38 52 62 75 85 98 107 121 135 T89-35 29 40 54 64 76 88 101 110 124 139 T89-36 24 34 43 52 63 72 84 95 108 120 T89-37 27 40 52 63 73 84 97 105 122 137 T89-38 13 14 18 22 39 46 55 62 72 81 T89-39 28 39 51 60 73 85 98 106 120 134 T89-40 23 42 45 54 68 75 86 97 108 122 T89-41 29 40 53 63 75 85 99 107 117 131 T89-42 33 43 58 69 83 97 111 121 136 150 T89-45 23 36 48 58 69 78 93 103 119 N/A T89-46 25 34 44 51 60 68 77 87 99 113 T89-69 28 38 49 61 75 86 100 110 126 142

TABLE-US-00097 TABLE 2.47 Diameter growth data for TF0173 Diameter (mm) Days in greenhouse 34 45 48 56 TF0173-3A-1 5.6 6.4 7.3 8.0 TF0173-3A-2 6.5 6.8 7.3 9.2 TF0173-3B-1 6.0 8.9 9.0 9.8 TF0173-3B-2 5.7 8.2 8.7 9.5 TF0173-4A-1 6.0 7.9 8.5 10.1 TF0173-4A-2 5.6 8.0 8.5 10.7 TF0173-4B-1 6.3 8.1 9.0 11.5 TF0173-4B-2 5.2 6.8 8.7 9.3 T89-01 7.0 N/A 9.0 10.2 T89-02 5.9 6.0 6.4 6.8 T89-03 6.2 7.9 10.3 9.8 T89-04 6.2 9.0 8.2 9.5 T89-05 6.2 8.0 8.4 9.8 T89-06 6.6 8.9 9.7 9.9 T89-07 5.2 6.3 6.7 7.0 T89-08 5.7 6.9 7.9 9.1 T89-09 4.1 7.6 9.0 9.8 T89-10 6.3 8.5 8.4 9.9 T89-12 5.8 7.8 8.3 9.7 T89-13 5.7 7.8 8.0 9.3 T89-14 6.5 6.8 7.1 8.8 T89-15 6.0 7.6 8.3 9.5 T89-16 6.3 8.6 7.8 9.3 T89-18 7.2 7.7 8.4 8.3 T89-19 6.1 8.1 8.7 9.4 T89-20 6.8 8.0 8.2 9.7 T89-21 6.3 7.3 8.3 9.6 T89-23 5.7 7.9 7.8 9.6 T89-24 5.7 6.9 7.1 8.4 T89-25 5.7 8.0 9.2 10.3 T89-26 6.5 8.7 9.5 10.3 T89-27 6.4 7.7 8.6 10.1 T89-31 5.6 8.6 7.7 9.2 T89-32 7.2 9.0 9.2 10.6 T89-35 6.9 8.1 8.6 9.6 T89-36 5.3 7.4 8.2 9.7 T89-37 5.5 8.0 8.7 9.2 T89-38 5.4 N/A 6.1 7.0 T89-39 6.0 8.3 8.8 10.4 T89-40 5.3 7.0 7.7 8.7 T89-41 5.4 7.4 7.8 9.3 T89-42 6.2 8.5 9.1 10.5 T89-45 5.4 8.5 8.7 9.9 T89-46 7.3 7.4 8.0 9.6 T89-69 5.7 8.0 8.7 8.9

[0328] Results from growth analysis are specified in the overview table 2.48. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00098 TABLE 2.48 Overview table of growth effects of construct TF0173 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0173 1.06 1.04 1.07 1.19 1.03 1.09 1.04 0.99

Construction Group TF0247

[0329] This construct induces increased growth. The final height is 7% higher comparing the average of the construction group and wild type control group. The final height is 10% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 5% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 7% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 18% higher comparing the average of the construction group and wild type control group. The final diameter is 9% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 22% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 18% higher comparing the largest individuals of the construction group and wild type control group. The TF0247 construction group meets the more stringent level of growth difference selection criterion (2) and the less stringent level of growth difference selection criteria (1), (3) and (4) as shown in table 2.51.

[0330] Tables 2.49 and 2.50 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00099 TABLE 2.49 Height growth data for TF0247 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0247-1A 18 29 40 52 67 76 98 123 131 TF0247-3A 22 38 51 63 78 90 118 145 154 TF0247-3B 23 37 50 61 76 87 114 139 145 TF0247-4A 17 30 41 53 68 77 103 127 134 TF0247-6B 25 40 52 63 73 83 97 111 116 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00100 TABLE 2.50 Diameter growth data for TF0247 Diameter (mm) Days in greenhouse 32 39 46 53 55 TF0247-1A 4.9 6.0 7.4 8.5 9.7 TF0247-3A 5.8 6.8 7.6 8.7 9.1 TF0247-3B 5.4 7.0 8.1 9.5 10.0 TF0247-4A 4.6 5.7 7.4 10.3 9.7 TF0247-6B 6.0 6.8 7.0 7.3 7.9 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0331] Results from growth analysis are specified in the overview table 2.51. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00101 TABLE 2.51 Overview table of growth effects of construct TF0247 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0247 1.07 1.18 1.05 1.22 1.10 1.09 1.07 1.18

Construction Group TF0405

[0332] This construct induces increased growth. The final height is 10% higher comparing the average of the construction group and wild type control group. The final height is 9% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 13% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 15% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 15% higher comparing the average of the construction group and wild type control group. The final diameter is 10% higher comparing the largest individuals of the construction group and wild type control group. The diameter growth rate is 22% higher comparing the average of the construction group and wild type control group. The diameter growth rate is 19% higher comparing the largest individuals of the construction group and wild type control group. The TF0405 construction group meets the more stringent level of growth difference selection criteria (1) and (2) and the less stringent level of growth difference selection criteria (3) and (4) as shown in table 2.54.

[0333] Tables 2.52 and 2.53 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00102 TABLE 2.52 Height growth data for TF0405 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TF0405-2A-1 7 17 25 36 46 57 83 109 117 TF0405-2A-2 21 36 50 60 74 87 118 146 153 TF0405-2B-2 19 34 47 58 73 84 108 130 136 TF0405-3A-1 24 41 54 63 77 88 117 144 150 TF0405-3A-2 25 38 52 64 79 91 117 142 150 TF0405-3B-1 19 33 44 54 65 75 98 123 130 TF0405-3B-2 22 36 49 59 72 86 112 136 145 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00103 TABLE 2.53 Diameter growth data for TF0405 Diameter (mm) Days in greenhouse 32 39 46 53 55 TF0405-2A-1 4.0 5.1 6.8 9.1 9.2 TF0405-2A-2 5.3 6.9 8.3 10.2 10.1 TF0405-2B-2 5.3 7.0 7.7 8.9 9.0 TF0405-3A-1 5.2 6.9 8.1 9.1 8.9 TF0405-3A-2 5.4 6.7 8.0 9.2 9.0 TF0405-3B-1 5.1 5.8 7.3 7.8 7.9 TF0405-3B-2 5.3 6.3 7.8 9.2 9.1 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0334] Results from growth analysis are specified in the overview table 2.54. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00104 TABLE 2.54 Overview table of growth effects of construct TF0405 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TF0405 1.10 1.15 1.13 1.22 1.09 1.10 1.15 1.19

Construction Group TFSTT004

[0335] Tables 2.55 and 2.56 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00105 TABLE 2.55 Height growth data for TFSTT004 Height (cm) Days in greenhouse 19 28 34 40 44 47 51 54 61 65 TFSTT004-1A 18 35 55 71 84 95 105 114 135 145 TFSTT004-2A-1 16 33 54 67 82 89 100 108 130 141 TFSTT004-2A-2 19 39 57 71 84 94 106 112 130 140 TFSTT004-2B-1 20 41 61 78 90 99 110 119 139 150 TFSTT004-2B-2 20 38 59 74 85 93 103 113 135 146 TFSTT004-3B 19 35 53 66 77 87 98 107 124 134 TFSTT004-4B-1 18 33 51 63 76 86 98 108 129 142 TFSTT004-4B-2 18 35 57 74 86 96 108 116 141 152 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

TABLE-US-00106 TABLE 2.56 Diameter growth data for TFSTT004 Diameter (mm) Days in greenhouse 28 34 40 44 47 51 54 61 65 TFSTT004-1A 4.6 6.0 8.5 7.9 8.6 9.0 10.3 11.2 11.3 TFSTT004-2A-1 4.6 5.7 6.5 7.4 8.8 9.1 9.8 10.6 10.7 TFSTT004-2A-2 4.2 5.9 6.9 7.5 8.5 8.9 9.6 10.2 10.3 TFSTT004-2B-1 5.0 6.5 7.8 8.9 9.6 10.9 10.9 12.1 12.6 TFSTT004-2B-2 4.5 6.2 7.3 8.3 8.3 9.3 8.9 10.7 11.0 TFSTT004-3B 4.1 5.6 6.8 7.5 8.2 9.0 9.9 10.6 10.7 TFSTT004-4B-1 3.6 5.0 6.0 7.0 7.5 9.3 9.2 10.6 11.0 TFSTT004-4B-2 4.2 5.7 7.5 8.6 9.2 9.5 10.4 11.1 11.7 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 N/A 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[0336] Results from growth analysis are specified in the overview table 2.57. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00107 TABLE 2.57 Overview table of growth effects of construct TFSTT004 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT004 1.15 1.17 1.06 1.09 1.09 1.02 0.97 0.91

Construction Group TFSTT013

[0337] The gene over-expressed with construct TFSTT013 generates the same top hits as the gene over-expressed with construct TFSTT038, when using BLAST search against the P. trichocarpa Jamboree Gene Model database at the Joint Genome Institute web page (http://genome.jgi-psf.org/cgi-bin/runAlignment?db=Poptr1_--1), indicating high homology between the two genes.

[0338] Tables 2.58 and 2.59 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00108 TABLE 2.58 Height growth data for TFSTT013 Height (cm) Days in greenhouse 19 25 32 39 48 54 56 TFSTT013-1A 18 30 54 81 113 137 140 TFSTT013-1B 19 31 54 75 108 124 127 TFSTT013-2B 16 28 48 67 98 115 121 TFSTT013-3A 17 29 48 70 89 103 110 TFSTT013-3B 20 32 53 75 106 128 136 TFSTT013-4A 23 37 59 83 116 141 149 TFSTT013-4B 21 33 57 85 120 140 147 TFSTT013-5B 18 30 52 77 109 126 133 T89-01 18 28 49 70 100 126 133 T89-02 19 29 51 76 105 128 136 T89-03 11 18 36 47 76 93 98 T89-04 16 25 48 74 102 126 133 T89-05 17 29 49 73 106 128 135 T89-06 16 28 51 74 105 127 132 T89-07 19 28 51 73 104 125 136 T89-08 19 31 52 77 110 130 137 T89-09 17 26 44 67 93 114 122 T89-10 16 25 44 63 89 108 115 T89-11 20 31 47 68 87 114 118 T89-12 17 28 48 70 101 117 124 T89-13 18 30 52 73 105 125 130 T89-14 19 29 44 67 96 116 123 T89-15 17 29 49 72 102 124 131 T89-16 18 29 50 74 108 129 135 T89-17 14 23 43 65 93 116 123 T89-18 15 26 48 72 103 128 136 T89-19 11 19 38 63 93 115 122 T89-20 19 30 52 75 104 126 133 T89-21 17 28 48 72 104 128 135 T89-22 19 29 49 73 105 123 129 T89-23 21 30 54 79 112 135 138 T89-24 19 24 37 54 77 100 103 T89-25 15 27 46 69 98 117 123 T89-26 19 29 50 73 103 127 135 T89-27 15 27 47 70 106 129 135 T89-28 20 33 55 80 109 130 137 T89-29 24 38 62 88 122 143 151 T89-30 21 33 56 78 113 132 138 T89-31 21 31 52 76 105 126 133 T89-32 18 29 50 73 105 129 138 T89-33 23 37 61 89 121 143 150 T89-34 19 30 52 78 108 130 138 T89-35 19 31 55 79 111 134 142 T89-36 18 29 52 75 105 127 134 T89-37 22 32 52 73 103 124 130 T89-38 18 29 51 74 105 128 134 T89-39 21 31 51 72 102 119 124 T89-40 20 32 51 74 99 121 128 T89-41 19 29 50 73 108 130 135 T89-42 15 26 46 68 95 113 119 T89-43 16 28 48 67 93 113 119 T89-44 21 31 50 70 97 116 122 T89-45 16 26 48 72 101 123 131 T89-46 21 29 50 75 106 129 138 T89-47 9 16 34 55 82 106 114 T89-48 5 8 17 24 42 52 56 T89-49 20 30 50 71 96 117 125 T89-50 16 28 50 74 105 128 136 T89-51 19 29 51 75 107 128 136 T89-52 20 29 50 75 104 124 131 T89-53 21 32 50 72 101 118 125 T89-54 21 32 55 81 112 133 142 T89-55 21 32 56 83 112 133 141

TABLE-US-00109 TABLE 2.59 Diameter growth data for TFSTT013 Days in Diameter (mm) greenhouse 39 48 54 56 TFSTT013-1A 5.9 7.9 8.6 9.4 TFSTT013-1B 5.9 8.4 8.5 8.6 TFSTT013-2B 5.7 5.6 7.8 8.1 TFSTT013-3A 6.4 6.9 7.5 7.8 TFSTT013-3B 5.9 7.9 8.8 9.8 TFSTT013-4A 6.8 8.3 9.0 9.9 TFSTT013-4B 7.2 9.1 10.4 10.1 TFSTT013-5B 7.5 9.0 10.3 9.5 T89-01 6.6 8.4 8.5 9.2 T89-02 6.8 8.3 9.4 10.1 T89-03 4.6 4.9 6.1 6.1 T89-04 6.9 8.7 10.0 9.4 T89-05 5.6 7.6 8.2 8.8 T89-06 6.9 8.2 9.6 9.3 T89-07 6.6 7.9 9.5 9.5 T89-08 6.9 8.9 9.6 9.8 T89-09 6.1 7.6 8.6 8.7 T89-10 5.5 7.5 8.6 8.6 T89-11 7.4 8.4 9.3 8.7 T89-12 5.9 7.4 8.7 9.5 T89-13 6.2 7.7 9.2 9.5 T89-14 5.9 7.8 9.2 8.7 T89-15 6.8 8.1 9.4 9.7 T89-16 7.3 9.2 10.6 10.3 T89-17 6.4 8.0 8.9 9.1 T89-18 7.0 7.5 8.1 8.8 T89-19 5.6 7.4 8.6 8.9 T89-20 6.5 8.3 8.6 9.5 T89-21 6.2 8.1 8.0 8.7 T89-22 6.2 8.5 9.3 10.1 T89-23 6.2 8.1 9.2 9.5 T89-24 3.1 4.6 5.0 4.5 T89-24 3.1 4.6 5.0 4.5 T89-25 5.9 7.7 8.5 8.9 T89-26 6.2 7.8 8.6 9.5 T89-27 6.1 7.6 8.2 8.6 T89-28 6.9 9.0 10.7 10.5 T89-29 7.8 8.4 10.3 10.2 T89-30 6.4 8.5 9.4 9.9 T89-31 6.8 8.6 8.8 9.5 T89-32 6.7 8.4 10.2 10.9 T89-33 7.1 8.7 9.4 11.4 T89-34 7.1 8.5 11.1 10.9 T89-35 6.7 9.0 8.9 11.4 T89-36 6.2 8.0 8.5 8.5 T89-37 5.6 7.1 7.8 8.7 T89-38 5.8 7.8 8.8 8.7 T89-39 6.2 8.1 9.2 9.3 T89-40 6.2 7.9 9.6 9.5 T89-41 5.9 8.2 8.9 9.5 T89-42 6.8 8.4 9.1 10.4 T89-43 6.7 8.5 9.7 9.3 T89-44 7.1 9.1 9.2 9.4 T89-45 6.6 7.8 9.2 9.2 T89-46 6.8 9.3 10.2 10.0 T89-47 5.3 7.0 7.9 8.4 T89-48 2.3 3.0 3.2 3.5 T89-49 7.5 8.7 9.1 9.8 T89-50 6.8 8.6 9.3 8.9 T89-51 7.2 8.3 9.2 9.1 T89-52 7.5 8.8 9.4 9.7 T89-53 7.3 9.3 9.5 10.2 T89-54 7.0 9.0 9.2 9.6 T89-55 7.0 8.9 9.2 9.8

[0339] Results from growth analysis are specified in the overview table 2.60. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00110 TABLE 2.60 Overview table of growth effects of construct TFSTT013 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT013 1.03 0.99 1.01 0.96 0.99 0.88 1.01 0.87

Construction Group TFSTT016

[0340] Tables 2.61 and 2.62 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00111 TABLE 2.61 Height growth data for TFSTT016 Height (cm) Days in greenhouse 19 28 34 40 44 47 51 54 61 65 TFSTT016-1A 19 37 57 71 83 91 101 111 127 137 TFSTT016-1B 19 36 54 67 78 87 98 107 126 138 TFSTT016-2A 18 34 51 67 76 84 93 101 118 130 TFSTT016-2B 18 34 55 70 80 87 97 105 125 137 TFSTT016-3A-1 19 34 51 65 76 84 95 106 125 137 TFSTT016-3A-2 17 33 50 64 76 85 96 107 129 143 TFSTT016-4A 16 33 52 69 83 91 103 112 136 151 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

TABLE-US-00112 TABLE 2.62 Diameter growth data for TFSTT016 Diameter (mm) Days in greenhouse 28 34 40 44 47 51 54 61 65 TFSTT016-1A 3.9 5.8 7.3 8.5 8.1 9.4 9.6 9.9 10.7 TFSTT016-1B 4.1 5.7 6.4 7.2 8.3 9.1 9.3 10.9 11.1 TFSTT016-2A 3.6 5.5 6.4 7.2 8.3 8.6 9.2 10.0 10.5 TFSTT016-2B 4.3 5.7 7.0 7.5 7.8 7.9 8.6 8.9 9.1 TFSTT016-3A-1 3.8 5.6 6.7 7.8 8.9 9.9 10.7 11.4 11.7 TFSTT016-3A-2 3.6 5.0 6.5 7.7 7.8 9.3 9.1 10.4 10.9 TFSTT016-4A 4.2 5.2 6.6 8.0 8.6 9.7 9.7 11.3 11.5 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 N/A 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[0341] Results from growth analysis are specified in the overview table 2.63. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00113 TABLE 2.63 Overview table of growth effects of construct TFSTT016 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT016 1.11 1.13 1.03 1.09 1.09 0.95 1.04 0.96

Construction Group TFSTT0191Rp1

[0342] Tables 2.64 and 2.65 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00114 TABLE 2.64 Height growth data for TFSTT019Rp1 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TFSTT019rp1-2A-1 17 24 32 45 58 71 81 88 98 104 TFSTT019rp1-2A-2 15 23 30 42 57 72 80 88 99 108 TFSTT019rp1-2A-3 16 22 28 40 55 67 78 87 97 105 TFSTT019rp1-3A-1 16 24 32 47 62 79 87 96 104 111 TFSTT019rp1-3A-2 14 20 24 28 38 54 61 69 79 85 TFSTT019rp1-3A-3 18 30 35 47 62 79 89 97 107 116 TFSTT019rp1-4BA-1 18 23 29 40 54 66 78 86 96 104 TFSTT019rp1-4BA-2 19 27 35 48 65 79 92 101 112 121 TFSTT019rp1-4BA-3 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A TFSTT019rp1-4BB-1 18 27 35 50 65 80 90 99 111 117 TFSTT019rp1-4BB-2 17 23 31 43 57 70 80 86 96 104 TFSTT019rp1-4BB-3 17 23 30 42 60 74 82 90 98 107 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00115 TABLE 2.65 Diameter growth data for TFSTT019Rp1 Diameter (mm) Days in greenhouse 35 41 46 53 60 TFSTT019rp1-2A-1 5.4 6.1 7.3 8.2 8.5 TFSTT019rp1-2A-2 5.2 6.6 8.0 8.9 9.3 TFSTT019rp1-2A-3 5.0 6.4 7.1 8.9 9.0 TFSTT019rp1-3A-1 5.5 6.5 7.4 8.2 8.9 TFSTT019rp1-3A-2 4.2 5.8 5.9 6.0 6.9 TFSTT019rp1-3A-3 5.1 6.6 7.8 8.9 9.4 TFSTT019rp1-4BA-1 4.8 6.5 8.5 7.9 9.1 TFSTT019rp1-4BA-2 5.0 6.4 6.9 8.3 9.2 TFSTT019rp1-4BA-3 N/A N/A N/A N/A N/A TFSTT019rp1-4BB-1 5.2 6.6 7.5 8.1 8.8 TFSTT019rp1-4BB-2 4.7 N/A 7.1 8.2 8.6 TFSTT019rp1-4BB-3 5.5 6.9 7.5 8.1 8.8 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 N/A 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0343] Results from growth analysis are specified in the overview table 2.66. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00116 TABLE 2.66 Overview table of growth effects of construct TFSTT019Rp1 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT019Rp1 0.99 1.08 0.98 0.99 0.95 1.00 0.93 0.88

Construction Group TFSTT036

[0344] This construct induces increased growth. The final height is 10% higher comparing the average of the construction group and wild type control group. The final height is 8% higher comparing the largest individuals of the construction group and wild type control group. The maximum height growth rate is 14% higher comparing the average of the construction group and wild type control group. The maximum height growth rate is 12% higher comparing the largest individuals of the construction group and wild type control group. The final diameter is 7% higher comparing the average of the construction group and wild type control group. The final diameter is 14% higher comparing the largest individuals of the construction group and wild type control group. The TFSTT036 construction group meets growth difference selection criterion (1) as shown in table 2.69.

[0345] Tables 2.67 and 2.68 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00117 TABLE 2.67 Height growth data for TFSTT036 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TFSTT036-1B 23 37 46 56 68 79 103 126 132 TFSTT036-2A 18 36 46 57 72 83 108 126 132 TFSTT036-2B 21 33 45 54 67 80 111 135 143 TFSTT036-3A 21 36 48 59 77 90 113 133 140 TFSTT036-4A 25 39 51 61 76 88 117 141 151 TFSTT036-4B 23 38 53 67 83 92 119 144 148 TFSTT036-5B 21 33 44 55 69 81 106 130 139 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00118 TABLE 2.68 Diametergrowth data for TFSTT036 Days in Diameter (mm) greenhouse 32 39 46 53 55 TFSTT036-1B 5.2 6.6 7.4 8.1 8.5 TFSTT036-2A 5.8 6.8 8.0 9.5 8.7 TFSTT036-2B 3.9 5.1 6.2 7.5 7.5 TFSTT036-3A 4.2 4.2 5.1 6.2 6.4 TFSTT036-4A 6.3 6.9 8.9 10.1 10.5 TFSTT036-4B 5.5 6.8 8.2 8.7 8.4 TFSTT036-5B 5.7 6.8 7.6 8.7 8.9 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0346] Results from growth analysis are specified in the overview table 2.69. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00119 TABLE 2.69 Overview table of growth effects of construct TFSTT036 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT036 1.10 1.07 1.14 0.99 1.08 1.14 1.12 0.95

Construction Group TFSTT038

[0347] The gene over-expressed with construct TFSTT038 generates the same top hits as the gene over-expressed with construct TFSTT013, when using BLAST search against the P. trichocarpa Jamboree Gene Model database at the Joint Genome Institute web page (http://genome.jgi-psf.org/cgi-bin/runAlignment?db=Poptr1_--1), indicating high homology between the two genes.

[0348] Tables 2.70 and 2.71 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00120 TABLE 2.70 Height growth data for TFSTT038 Height (cm) Days in greenhouse 19 28 34 40 44 47 51 54 61 65 TFSTT038-1A-1 23 42 62 82 92 100 110 118 138 153 TFSTT038-1A-2 19 38 58 70 83 90 102 110 130 142 TFSTT038-1B 18 33 48 62 73 83 93 99 117 128 TFSTT038-2A 21 38 58 71 85 96 108 119 143 157 TFSTT038-2B 14 29 46 60 70 78 87 96 118 130 TFSTT038-3A 18 38 57 70 83 92 105 116 133 142 TFSTT038-3B 21 38 57 72 84 92 102 110 122 126 TFSTT038-4B 17 34 52 67 78 87 96 104 123 133 T89-01 18 30 46 58 69 77 87 96 113 122 T89-02 18 30 49 62 72 77 84 90 102 109 T89-03 15 27 41 54 65 73 82 91 112 123 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 19 31 49 61 74 83 94 103 124 133 T89-12 17 30 45 58 69 77 90 100 123 134 T89-13 18 27 43 56 68 78 91 100 121 133 T89-14 5 26 42 56 67 74 83 90 109 119 T89-15 10 15 25 33 41 45 52 57 72 82 T89-16 19 33 53 63 73 82 93 102 119 130 T89-17 17 29 46 58 66 72 80 86 96 102 T89-18 16 30 48 59 71 81 93 103 122 132 T89-19 18 32 50 65 77 84 94 102 126 139 T89-20 16 33 52 67 79 88 98 105 124 139 T89-21 17 29 44 58 67 73 83 90 110 122 T89-22 14 28 47 61 71 80 89 98 119 133 T89-23 10 33 45 53 63 71 82 90 112 123 T89-24 20 28 43 51 62 69 78 87 106 119 T89-25 14 26 38 52 64 72 82 89 110 122 T89-26 15 28 44 57 69 77 87 96 120 133 T89-27 18 29 47 62 75 82 92 103 125 138 T89-28 22 37 54 67 78 88 97 104 123 133 T89-29 16 33 49 63 76 84 93 100 123 138 T89-30 15 40 46 58 67 74 85 92 113 124

TABLE-US-00121 TABLE 2.71 Diameter growth data for TFSTT038 Diameter (mm) Days in greenhouse 28 34 40 44 47 51 54 61 65 TFSTT038-1A-1 4.4 6.1 7.4 8.4 9.3 9.8 10.5 11.3 11.8 TFSTT038-1A-2 3.8 5.5 6.6 7.7 7.9 8.9 9.4 10.6 11.2 TFSTT038-1B 3.4 5.1 6.5 7.2 7.8 8.3 10.1 9.3 9.5 TFSTT038-2A 3.8 5.4 6.7 7.2 8.7 9.6 9.8 11.5 11.3 TFSTT038-2B 3.8 4.4 5.4 6.4 7.2 7.8 8.5 10.1 10.7 TFSTT038-3A 3.9 5.7 7.4 7.8 8.4 9.3 9.8 10.6 11.1 TFSTT038-3B 4.3 5.9 6.8 7.8 8.0 8.0 8.4 9.0 9.9 TFSTT038-4B 4.2 4.5 6.3 6.8 7.3 8.2 8.6 9.0 9.1 T89-01 3.2 4.6 5.4 6.2 6.8 7.9 8.3 9.5 9.3 T89-02 3.4 4.7 5.5 7.3 6.3 6.6 6.9 8.3 7.5 T89-03 3.9 4.4 5.2 6.2 6.4 7.8 7.6 9.7 9.4 T89-10 N/A N/A N/A N/A N/A N/A N/A N/A N/A T89-11 3.4 5.1 6.2 7.6 6.8 6.9 7.7 10.1 9.5 T89-12 2.9 4.7 5.8 6.6 7.8 8.0 8.7 9.1 9.2 T89-13 3.0 4.3 5.4 5.8 6.4 7.6 7.8 8.2 8.8 T89-14 3.0 4.5 5.7 6.3 6.7 7.9 7.8 9.0 8.9 T89-15 N/A 2.1 3.0 4.0 4.0 4.5 4.9 5.5 5.6 T89-16 3.5 5.3 6.4 6.6 7.0 7.2 8.3 8.2 9.0 T89-17 3.4 4.6 5.1 5.4 6.0 6.4 6.5 6.8 7.1 T89-18 3.6 5.2 6.0 7.0 7.8 8.2 9.9 10.3 9.7 T89-19 4.2 5.5 6.6 7.7 8.5 8.9 9.5 11.1 12.3 T89-20 4.1 5.5 6.6 8.1 9.3 9.6 9.3 10.0 11.1 T89-21 3.1 5.6 5.8 6.7 7.1 7.8 8.4 9.7 10.1 T89-22 3.2 4.4 5.6 6.5 7.5 7.6 7.8 8.9 9.2 T89-23 2.4 4.2 5.1 6.1 6.5 7.5 10.1 9.3 10.2 T89-24 3.2 4.5 5.1 6.3 7.0 7.6 8.1 8.8 9.1 T89-25 3.3 4.3 5.2 5.8 6.5 7.4 7.8 9.3 9.7 T89-26 3.3 4.4 5.5 6.6 7.2 8.1 8.9 9.5 10.5 T89-27 3.3 4.9 6.0 7.8 8.0 8.9 9.7 11.2 11.5 T89-28 4.5 5.7 7.4 7.8 8.5 9.4 9.7 10.2 11.1 T89-29 3.1 4.7 6.3 7.2 7.9 9.2 9.8 11.1 10.7 T89-30 3.0 5.8 6.2 7.7 7.9 8.6 8.2 10.1 10.3

[0349] Results from growth analysis are specified in the overview table 2.72. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00122 TABLE 2.72 Overview table of growth effects of construct TFSTT038 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT038 1.11 1.11 1.04 1.06 1.13 0.96 1.05 0.92

Construction Group TFSTT045

[0350] Tables 2.73 and 2.74 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00123 TABLE 2.73 Height growth data for TFSTT045 Height (cm) Days in greenhouse 18 25 28 32 36 39 46 53 55 TFSTT045-1B 19 33 43 52 64 75 94 116 123 TFSTT045-2B 24 37 49 58 71 80 105 127 133 TFSTT045-3A 20 35 48 60 76 87 113 143 153 TFSTT045-3B 16 28 37 47 57 65 86 111 118 TFSTT045-4A 23 34 45 56 70 82 105 133 139 TFSTT045-4B 25 38 51 60 75 87 109 119 120 TFSTT045-7B 23 35 46 60 70 80 N/A 124 130 T89-01 18 32 43 54 66 76 98 124 133 T89-02 20 33 43 53 67 76 101 124 129 T89-03 17 31 43 53 68 76 102 128 135 T89-04 19 33 44 54 67 74 97 122 129 T89-05 20 33 44 54 67 76 100 121 129 T89-06 18 31 42 53 67 76 97 121 128 T89-07 16 28 41 53 65 74 96 121 129 T89-08 19 34 43 53 65 75 98 123 131 T89-09 20 30 40 52 64 73 97 119 125 T89-10 21 33 41 49 61 70 92 114 121 T89-11 18 31 40 49 61 71 94 117 123 T89-12 18 31 40 N/A 60 68 90 107 115 T89-13 19 35 47 58 70 78 103 128 135 T89-14 19 31 40 50 61 72 93 118 124 T89-15 20 32 41 50 62 70 91 114 120 T89-16 18 30 39 49 62 71 92 114 122 T89-17 17 33 45 55 70 78 104 129 136 T89-18 19 32 44 56 71 82 106 130 137 T89-19 16 26 36 46 51 69 89 112 119 T89-20 16 31 41 49 60 71 91 111 118 T89-21 20 31 44 54 68 76 99 122 127 T89-22 14 26 37 46 61 72 96 120 127 T89-23 19 32 40 50 63 73 100 121 127 T89-24 20 31 41 47 58 68 90 111 117 T89-25 20 36 45 57 69 78 101 127 134 T89-26 20 37 49 58 71 80 107 131 140 T89-27 19 34 44 55 71 81 107 131 138 T89-28 17 35 44 56 68 76 99 126 132 T89-29 17 32 45 55 68 78 101 125 132 T89-30 18 31 41 50 63 72 95 119 125 T89-31 17 27 35 45 58 67 87 108 116 T89-32 19 32 44 52 65 74 98 121 127 T89-33 20 33 43 53 64 74 96 119 126 T89-34 19 34 44 55 68 78 100 124 130 T89-35 17 30 40 51 64 74 95 116 122 T89-36 18 30 40 49 62 71 91 114 121 T89-82 21 33 43 54 65 75 98 118 125 T89-83 22 35 47 55 71 80 105 130 137 T89-85 21 36 46 56 67 76 98 123 131

TABLE-US-00124 TABLE 2.74 Diameter growth data for TFSTT045 Days in Diameter (mm) greenhouse 32 39 46 53 55 TFSTT045-1B 5.2 5.7 7.0 8.1 8.6 TFSTT045-2B 5.5 6.7 7.8 8.8 9.1 TFSTT045-3A 6.5 7.2 8.7 10.4 10.6 TFSTT045-3B 4.8 5.6 7.4 8.0 8.6 TFSTT045-4A 5.1 6.3 7.0 7.9 8.2 TFSTT045-4B 5.5 6.1 7.3 8.0 8.8 TFSTT045-7B 5.1 6.9 8.3 9.0 9.6 T89-01 5.2 6.0 6.8 8.2 7.6 T89-02 4.5 5.8 7.1 8.6 8.3 T89-03 4.4 5.7 6.8 7.9 8.1 T89-04 4.4 6.0 6.9 8.6 8.5 T89-05 4.6 5.8 6.7 7.9 8.0 T89-06 4.7 5.9 7.2 8.1 9.2 T89-07 4.8 5.5 6.2 6.9 7.1 T89-08 4.5 5.4 6.0 6.9 7.1 T89-09 4.7 5.6 6.7 8.8 8.0 T89-10 4.1 4.9 5.6 7.1 7.0 T89-11 3.8 5.3 7.3 8.0 7.6 T89-12 N/A 5.1 6.2 7.3 6.9 T89-13 4.8 5.7 6.5 7.6 7.6 T89-13 4.8 5.7 6.5 7.6 7.6 T89-14 4.2 5.5 6.6 7.6 6.5 T89-15 5.0 6.1 7.2 7.9 8.3 T89-16 4.4 5.6 6.2 7.3 7.9 T89-17 4.8 6.7 7.0 8.3 8.4 T89-18 4.2 5.5 6.7 7.3 7.4 T89-19 4.7 5.5 6.8 7.2 7.8 T89-20 4.5 5.6 6.5 7.0 7.3 T89-21 4.9 5.6 6.4 7.4 7.6 T89-22 4.3 5.4 6.5 7.3 7.6 T89-23 3.9 5.5 6.7 7.7 7.0 T89-24 4.3 5.9 6.8 8.1 7.9 T89-25 4.7 6.5 7.5 9.2 9.1 T89-26 5.4 5.9 7.7 8.6 8.8 T89-27 4.7 5.6 7.4 7.9 8.0 T89-28 4.7 5.7 6.3 7.2 7.4 T89-29 4.8 5.6 6.6 7.7 8.1 T89-30 4.6 5.5 6.7 7.3 7.3 T89-31 4.3 5.8 6.3 7.5 7.6 T89-32 4.6 5.9 7.6 9.3 9.1 T89-33 4.4 5.3 6.2 7.1 7.1 T89-34 4.6 5.6 6.8 8.1 8.7 T89-35 5.3 6.0 7.5 8.8 8.9 T89-36 4.4 6.1 6.9 8.1 8.6 T89-82 4.7 5.3 6.5 7.5 7.4 T89-83 4.7 6.1 7.1 8.3 8.3 T89-85 5.1 6.3 7.3 7.7 8.2

[0351] Results from growth analysis are specified in the overview table 2.75. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00125 TABLE 2.75 Overview table of growth effects of construct TFSTT045 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT045 1.03 1.15 1.03 1.14 1.09 1.16 1.11 0.94

Construction Group TFSTT051 Rp1

[0352] Tables 2.76 and 2.77 contain growth data for specified construction group and corresponding wild type group. Table rows contain height and diameter measurements of individuals of specified construction group and corresponding wild type group. Time of measurement as number of days in greenhouse is shown in table headers.

TABLE-US-00126 TABLE 2.76 Height growth data for TFSTT051Rp1 Height (cm) Days in greenhouse 20 25 29 35 41 46 50 53 57 60 TFSTT051rp1-3B-1 19 25 29 44 60 70 76 83 90 97 TFSTT051rp1-3B-2 17 24 30 43 56 68 75 82 89 97 TFSTT051rp1-3B-3 18 24 28 37 49 63 69 75 83 90 TFSTT051rp1-4A-1 18 23 30 43 58 71 81 90 101 110 TFSTT051rp1-4A-2 16 25 33 45 62 78 88 97 109 119 TFSTT051rp1-4A-3 20 28 38 54 73 90 101 110 122 132 T89-01 17 26 31 45 61 76 87 95 107 116 T89-02 16 21 26 38 53 67 76 83 N/A N/A T89-03 18 24 32 47 64 78 87 96 106 115 T89-04 18 26 35 50 66 80 87 96 107 115 T89-05 17 26 33 44 57 73 81 91 100 107 T89-06 16 21 28 40 55 74 79 89 99 106 T89-07 17 23 31 43 57 71 80 88 98 107 T89-08 15 20 26 37 51 65 75 84 94 100 T89-09 18 25 32 46 61 74 85 93 103 112 T89-10 19 25 32 46 60 75 86 95 104 112 T89-11 20 27 36 51 68 86 98 107 120 127 T89-12 19 27 36 50 67 80 89 97 108 118 T89-13 18 25 32 45 59 75 83 92 101 108 T89-14 18 23 26 36 51 65 72 79 89 96 T89-15 17 21 28 39 53 70 78 87 97 105 T89-16 19 25 32 43 57 71 81 90 101 109 T89-17 16 20 29 41 54 68 78 84 93 101 T89-18 16 23 30 45 63 78 87 94 103 110 T89-19 16 22 28 42 56 69 79 89 100 107 T89-20 18 25 32 47 63 78 89 99 109 115 T89-21 19 27 34 50 67 82 92 102 111 120 T89-22 19 25 32 44 58 76 84 93 102 109 T89-23 18 26 33 47 63 79 88 99 108 116 T89-24 16 24 28 42 53 70 78 85 94 103 T89-25 16 25 32 45 61 76 85 95 107 114 T89-26 16 20 26 38 52 68 78 86 97 103 T89-27 16 21 25 35 48 61 70 78 N/A 98 T89-28 14 18 25 38 51 64 73 81 90 96 T89-29 7 13 18 30 45 60 69 77 90 98 T89-30 15 22 29 42 55 69 80 88 97 106 T89-31 21 29 37 50 65 80 88 98 108 116 T89-32 19 24 32 42 55 70 78 87 96 105

TABLE-US-00127 TABLE 2.77 Diameter growth data for TFSTT051Rp1 Days in Diameter (mm) greenhouse 35 41 46 53 60 TFSTT051rp1-3B-1 4.6 5.9 6.4 7.2 7.7 TFSTT051rp1-3B-2 4.3 5.6 6.0 6.1 7.3 TFSTT051rp1-3B-3 4.2 5.4 6.8 6.9 8.3 TFSTT051rp1-4A-1 4.5 5.9 6.6 7.8 9.1 TFSTT051rp1-4A-2 4.5 5.9 6.6 7.4 8.1 TFSTT051rp1-4A-3 5.2 6.4 7.1 7.9 8.3 T89-01 4.7 6.0 6.5 6.9 8.3 T89-02 4.4 5.7 6.6 7.6 7.8 T89-03 4.7 6.3 6.7 7.7 8.4 T89-04 4.7 6.0 7.1 8.0 8.5 T89-05 4.0 5.3 6.3 7.8 8.1 T89-06 4.6 5.9 6.3 7.7 8.2 T89-07 4.6 6.1 6.5 7.9 8.5 T89-08 4.0 5.2 5.8 6.9 7.1 T89-09 4.4 5.8 6.4 7.6 8.2 T89-10 4.9 6.0 6.6 7.4 8.5 T89-11 4.7 5.8 6.4 7.9 8.3 T89-12 4.7 5.9 7.3 7.4 7.6 T89-13 5.9 5.9 6.7 7.5 8.3 T89-14 4.5 5.2 5.8 6.1 7.0 T89-15 4.7 N/A 6.0 6.4 7.3 T89-16 4.5 5.6 7.7 7.5 8.6 T89-17 4.1 5.4 6.0 6.8 7.5 T89-18 4.4 5.8 6.0 7.7 8.3 T89-19 4.7 5.8 7.1 8.5 9.4 T89-20 4.7 5.8 6.6 7.4 8.3 T89-21 4.7 6.2 6.3 7.7 8.5 T89-22 4.2 5.1 6.3 6.9 8.0 T89-23 4.6 5.8 7.1 7.5 8.3 T89-24 4.4 5.8 6.9 7.7 8.8 T89-25 4.5 5.4 6.2 8.0 8.7 T89-26 4.1 5.4 6.0 7.2 8.0 T89-27 4.5 5.7 6.5 7.9 9.2 T89-28 4.3 5.1 6.0 6.6 7.7 T89-29 3.5 4.7 5.5 6.3 7.1 T89-30 4.1 5.4 5.8 7.2 7.8 T89-31 5.0 5.8 6.8 7.3 8.2 T89-32 4.4 6.4 6.6 8.0 8.4

[0353] Results from growth analysis are specified in the overview table 2.78. The determined growth effects of specified construction group are presented as ratios between construction and wild type group AFH, AFD, AMHGR, ADGR, MFH, MFD, MMHGR and MDC.

TABLE-US-00128 TABLE 2.78 Overview table of growth effects of construct TFSTT051Rp1 Maximum Average of Maximum Average Maximum Maximum Average Average Height Diameter Maximum Maximum Height Diameter Construction Final Final Growth Growth Final Final Growth Growth group Height Diameter Rate Rate Height Diameter Rate Rate TFSTT051Rp1 0.99 1.00 0.96 0.95 1.04 0.97 0.98 0.95

Example 3

Volumetric Growth Calculation

[0354] The volume of the stem of each individual plant is approximated from final height and final diameter measurements using cone volume.

Stem Volume Approximation

[0355] V = π * h * r 3 ##EQU00002##

where

V=Volume

[0356] h=height (Final height) r=radius (Final diameter/2)

[0357] Average final volumes of each construction group population and corresponding wild type population are subsequently calculated. A volumetric growth selection criterion is applied, where a construction group is considered having a significant or pronounced volume increase compared to the wild type population if construction group average final volume is at least 25% (or 50% in the second more stringent level) greater than corresponding wild type group average final volume.

[0358] Results from volume approximation are specified in the overview table 3.1. The determined growth effects are presented as ratios between construction and wild type group average final volumes AFV.

[0359] The following construction groups meet the volumetric growth criterion. Construction group TF0002Rp2 has an average final volume increase of 36%; construction group TF0013 has an average final volume increase of 27%; construction group TF0045 has an average final volume increase of 33%; construction group TF0096 has an average final volume increase of 44%; construction group TF0109 has an average final volume increase of 44%; construction group TF0116 has an average final volume increase of 31%; construction group TF0132rp1 has an average final volume increase of 46%, where construction group line TF0132rp1-4AC has an average final volume increase of 70% (+/-20%); construction group TF0146 has an average final volume increase of 34%; construction group TF0247 has an average final volume increase of 49%; construction group TF0405 has an average final volume increase of 45%; construction group TFSTT016 has an average final volume increase of 36%; construction group TFSTT036 has an average final volume increase of 28%; construction group TFSTT038 has an average final volume increase of 32%; construction group TFSTT045 has an average final volume increase of 38%.

[0360] The following construction groups meet the more stringent level of the volumetric growth criterion (5) as shown in table 3.1. Construction group TF0097Rp1 has an average final volume increase of 68%, where construction group line TF0097Rp1-3A has an average final volume increase of 116% (+/-37%); construction group TF0104 has an average final volume increase of 79%; construction group TF0109Rp1 has an average final volume increase of 58%, where construction group line TF0109Rp1-4A has an average final volume increase of 92% (+/-5%);%; construction group TF0132.2nd has an average final volume increase of 65%; construction group TFSTT004 has an average final volume increase of 51%. These construction groups meet the more stringent level of volumetric growth criterion (5) as shown in table 3.1.

TABLE-US-00129 TABLE 3.1 Average Construction group Final Volume TF0002Rp2 1.36 TF0013 1.27 TF0045 1.33 TF0096 1.44 TF0097Rp1 1.68 TF0104 1.79 TF0109 1.44 TF0109Rp1 1.58 TF0116 1.31 TF0132.2nd 1.65 TF0132rp1 1.46 TF0146 1.34 TF0247 1.49 TF0405 1.45 TFSTT004 1.51 TFSTT016 1.36 TFSTT036 1.28 TFSTT038 1.32 TFSTT045 1.38

Example 4

Dry Weight Measurements

[0361] Dry weight measurements were performed on replanted construction groups. Plants were harvested according to a standard procedure: stem, bark, five fully developed leafs, rest of leafs and roots were collected as separate samples. The leaf area were measured of the five fully developed leafs and the length of 20 fully developed internodes were measured. The separate samples of plant material were put in a drier oven for more than 48 hours. The dry weights were measured and analysed according differences compared to corresponding wild type groups. Abbreviations and parameters used for dry weight analyses is shown in table 4.1.

TABLE-US-00130 TABLE 4.1 Abbreviations and parameters associated with dry weight Stem (g) Dry weight of stem of one plant individual Bark (g) Dry weight of bark of one plant individual Root (g) Dry weight of root of one plant individual Leaf (g) Dry weight of all leafs of one plant individual TOTAL(g) Total dry weight of stem, bark and leafs of one plant individual SLA (m2/kg) Specific Leaf Area, square meter per kg leaf dry weight, calculated from five fully developed leaf of one plant individual Internode (cm) Average internode length, calculated from 20 internodes of one plant individual Average Stem Average stem dry weight of the wild type population and each construction group population Average Bark Average bark dry weight of the wild type population and each construction group population Average Root Average root dry weight of the wild type population and each construction group population Average Leaf Average Leaf dry weight of the wild type population and each construction group population Average TOTAL Average TOTAL dry weight of the wild type population and each construction group population Average SLA Average SLA of the wild type population and each construction group population Average Internode Average internode of the wild type population and each construction group population Maximum Stem Maximum stem dry weight of the wild type population and each construction group population Maximum Bark Maximum bark dry weight of the wild type population and each construction group population Maximum Root Maximum root dry weight of the wild type population and each construction group population Maximum Leaf Maximum Leaf dry weight of the wild type population and each construction group population Maximum TOTAL Maximum TOTAL dry weight of the wild type population and each construction group population Line Average Stem Average stem dry weight of the wild type population and each construction group line Line Average Bark Average bark dry weight of the wild type population and each construction group line Line Average Root Average root dry weight of the wild type population and each construction group line Line Average Leaf Average leaf dry weight of the wild type population and each construction group line Line Average TOTAL Average TOTAL dry weight of the wild type population and each construction group line Line Average SLA Average SLA of the wild type population and each construction group line Line Average Internode Average internode length of the wild type population and each construction group line

Dry Weight Experiment Results

Construction Group TF0013

[0362] This construct induce increase of biomass production. Dry weight measurements of the construction group show 20% increase of average stem, 14% increase of average bark, 14% increase of average leaf and 16% increase of average TOTAL compared to the corresponding wildtype group. One of the construction group lines show 48% increase of average stem, 37% increase of average bark, 31% increase of average leaf and 36% increase of average TOTAL compared to the corresponding wildtype group.

[0363] Table 4.2 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00131 TABLE 4.2 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL(g) (m2/kg) (cm) TF0013rp2-1A-1 4.4 2.1 15.1 21.6 33.7 3.0 TF0013rp2-1A-2 6.5 2.7 15.0 24.2 41.2 3.6 TF0013rp2-1A-3 5.9 2.8 14.4 23.0 33.6 3.5 TF0013rp2-3BA-1 8.7 3.7 19.5 31.9 34.4 3.8 TF0013rp2-3BA-2 9.1 3.9 20.6 33.6 32.8 3.7 TF0013rp2-3BA-3 8.7 3.7 19.8 32.2 34.2 4.1 T89-20 5.8 2.7 6.4 14.0 22.5 35.4 3.7 T89-21 5.9 3.0 8.5 16.9 25.8 30.3 3.2 T89-22 6.0 2.7 15.4 24.1 34.4 3.7 T89-23 4.3 2.0 4.6 12.1 18.4 48.9 3.3 T89-24 5.5 2.5 6.5 14.3 22.2 32.8 3.6 T89-25 7.6 3.4 8.0 18.8 29.8 31.2 3.3 T89-26 6.6 3.0 17.2 26.8 33.5 3.7 T89-27 6.9 3.1 16.0 26.0 31.4 3.8 T89-28 5.2 2.6 12.8 20.5 32.7 3.4

[0364] Table 4.3 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.3 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00132 TABLE 4.3 Average Average Average Average Average Average Average Construction Group Stem Bark Root Leaf TOTAL SLA Internode TF0013rp2 1.20 1.14 1.14 1.16 1.01 1.03

[0365] Table 4.4 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00133 TABLE 4.4 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0013rp2 1.20 1.16 1.09 1.13

[0366] Table 4.5 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.5 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00134 TABLE 4.5 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0013rp2-1A 0.93 0.91 0.97 0.95 1.05 0.96 TF0013rp2-3BA 1.48 1.37 1.31 1.36 0.98 1.10

Construction Group TF0132

[0367] This construct induce increase of biomass production. Dry weight measurements of the construction group show 83% increase of average stem, 58% increase of average bark, 34% increase of average leaf and 49% increase of average TOTAL compared to the corresponding wildtype group. One of the construction group lines show 119% increase of average stem, 82% increase of average bark, 53% increase of average leaf and 73% increase of average TOTAL compared to the corresponding wildtype group. For the lines were root dry weight were measured an increase in the shot-root ratio were observed.

[0368] Table 4.6 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00135 TABLE 4.6 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL(g) (m2/kg) (cm) TF0132rp1-1B-2 11.7 4.6 10.3 18.1 34.5 33.3 TF0132rp1-1B-3 9.1 3.5 6.0 15.7 28.4 33.3 4.2 TF0132rp1-3BB-1 9.8 4.1 10.7 18.7 32.6 33.7 4.2 TF0132rp1-3BB-2 11.4 4.9 9.8 24.8 41.2 30.8 4.3 TF0132rp1-3BB-3 10.8 4.3 10.7 20.4 35.5 34.1 4.3 TF0132rp1-4AC-1 13.3 5.1 20.7 39.1 32.8 3.6 TF0132rp1-4AC-2 14.5 5.5 26.7 46.7 34.2 3.9 TF0132rp1-4AC-3 11.4 4.5 22.5 38.4 34.6 3.7 TF0132rp1-4B-1 10.7 4.3 18.2 33.2 32.4 4.1 TF0132rp1-4B-2 6.7 2.9 16.9 26.4 34.3 4.0 TF0132rp1-4B-3 10.2 3.8 17.7 31.7 38.9 4.1 TF0132rp1-6B-1 7.5 3.6 21.3 32.5 32.5 4.0 TF0132rp1-6B-2 11.9 4.6 20.7 37.2 34.0 4.3 TF0132rp1-6B-3 11.1 4.6 22.5 38.2 31.4 4.3 T89-20 5.8 2.7 6.4 14.0 22.5 35.4 3.7 T89-21 5.9 3.0 8.5 16.9 25.8 30.3 3.2 T89-22 6.0 2.7 15.4 24.1 34.4 3.7 T89-23 4.3 2.0 4.6 12.1 18.4 48.9 3.3 T89-24 5.5 2.5 6.5 14.3 22.2 32.8 3.6 T89-25 7.6 3.4 8.0 18.8 29.8 31.2 3.3 T89-26 6.6 3.0 17.2 26.8 33.5 3.7 T89-27 6.9 3.1 16.0 26.0 31.4 3.8 T89-28 5.2 2.6 12.8 20.5 32.7 3.4

[0369] Table 4.7 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.7 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00136 TABLE 4.7 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TF0132rp1 1.83 1.58 1.58 1.34 1.49 0.96 1.17

[0370] Table 4.8 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00137 TABLE 4.8 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0132rp1 1.92 1.62 1.99 1.42 1.57

[0371] Table 4.9 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.9 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00138 TABLE 4.9 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0132rp1-1B 1.93 1.61 1.63 1.24 1.45 0.92 1.25 TF0132rp1-3BB 1.79 1.59 1.53 1.40 1.52 0.95 1.22 TF0132rp1-4AC 2.19 1.82 1.53 1.73 0.98 1.07 TF0132rp1-4B 1.54 1.32 1.15 1.27 1.02 1.15 TF0132rp1-6B 1.70 1.53 1.41 1.50 0.95 1.20

Construction Group TF0002

[0372] This construct induce increase of biomass production. Dry weight measurements of the construction group show 50% increase of average stem, 52% increase of average bark, 6% increase of average leaf and 20% increase of average TOTAL compared to the corresponding wildtype group. One of the construction group lines show 72% increase of average stem, 61% increase of average bark, 20% increase of average leaf and 35% increase of average TOTAL compared to the corresponding wildtype group. For the line were root dry weight were measured an increase in the shot-root ratio were observed.

[0373] Table 4.10 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00139 TABLE 4.10 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL(g) (m2/kg) (cm) TF0002rp2-1B-1 8.5 3.4 8.1 17.4 29.4 33.4 3.5 TF0002rp2-1B-2 10.7 4.7 12.6 20.5 35.8 32.2 3.2 TF0002rp2-1B-3 10.0 3.7 8.0 19.2 32.9 36.2 3.3 TF0002rp2-2A-1 8.0 3.4 14.5 25.9 34.3 3.3 TF0002rp2-2A-2 5.0 2.4 10.1 17.4 35.9 3.0 TF0002rp2-2A-3 6.4 3.3 12.2 22.0 25.3 3.1 TF0002rp2-3B-1 4.7 2.3 12.0 19.0 34.5 3.0 TF0002rp2-3B-2 10.7 4.7 20.7 36.1 32.1 3.3 TF0002rp2-3B-3 12.3 5.8 25.3 43.3 27.4 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0374] Table 4.11 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.11 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00140 TABLE 4.11 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TF0002rp2 1.50 1.52 1.16 1.06 1.20 0.94 1.19

[0375] Table 4.12 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00141 TABLE 4.12 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0002rp2 1.76 1.85 1.23 1.22 1.41

[0376] Table 4.13 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.13 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00142 TABLE 4.13 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0002rp2-1B 1.72 1.61 1.16 1.20 1.35 0.99 1.27 TF0002rp2-2A 1.15 1.23 0.77 0.90 0.93 1.18 TF0002rp2-3B 1.63 1.73 1.22 1.36 0.91 1.12

Construction Group TF0052

[0377] This construct induce increase of biomass production. Dry weight measurements of one of the construction group lines show 49% increase of average stem, 64% increase of average bark, 32% increase of average leaf and 38% increase of average TOTAL compared to the corresponding wildtype group.

[0378] Table 4.14 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00143 TABLE 4.14 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL(g) (m2/kg) (cm) TF0052rp1-2A-1 9.5 4.5 23.8 37.8 30.0 3.0 TF0052rp1-2A-2 7.3 3.6 18.4 29.2 33.7 3.0 TF0052rp1-2A-3 8.5 4.0 21.0 33.4 37.8 3.0 TF0052rp1-2B-1 4.7 2.2 14.1 21.0 39.8 2.6 TF0052rp1-2B-2 4.0 2.0 13.5 19.6 32.3 2.6 TF0052rp1-2B-3 2.4 1.3 7.3 11.0 31.0 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0379] Table 4.15 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.15 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00144 TABLE 4.15 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TF0052rp1 1.07 1.20 1.03 1.05 0.99 1.05

[0380] Table 4.16 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00145 TABLE 4.16 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0052rp1 1.36 1.43 1.15 1.23

[0381] Table 4.17 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.17 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00146 TABLE 4.17 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0052rp1-2A 1.49 1.64 1.32 1.38 0.99 1.12 TF0052rp1-2B 0.65 0.76 0.73 0.71 1.00 0.97

Construction Group TF0076

[0382] This construct induce increase of biomass production. Dry weight measurements of the construction group show 16% increase of average stem, 11% increase of average bark, 4% increase of average leaf and 7% increase of average TOTAL compared to the corresponding wildtype group. One of the construction group lines show 42% increase of average stem, 29% increase of average bark, 16% increase of average leaf and 23% increase of average TOTAL compared to the corresponding wildtype group. For the line were root dry weight were measured an increase in the shot-root ratio were observed.

[0383] Table 4.18 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00147 TABLE 4.18 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL(g) (m2/kg) (cm) TF0076rp2-3BB-1 5.8 2.7 15.7 24.2 32.9 3.0 TF0076rp2-3BB-2 6.9 3.1 17.0 27.1 29.3 2.7 TF0076rp2-3BB-3 8.0 3.5 23.1 34.6 33.0 2.8 TF0076rp2-4B-1 7.9 3.2 7.8 19.6 30.6 33.1 2.6 TF0076rp2-4B-2 7.5 3.2 7.7 18.0 28.7 35.4 2.7 TF0076rp2-4B-3 8.7 3.1 8.1 17.9 29.6 37.3 2.3 TF0076rp2-5BA-1 3.9 1.5 9.0 14.4 43.8 2.3 TF0076rp2-5BA-2 6.6 2.7 14.7 23.9 36.8 2.8 TF0076rp2-5BA-3 7.6 2.8 18.1 28.5 36.2 2.9 TF0076rp2-5BB-1 5.4 2.2 14.9 22.4 39.2 3.0 TF0076rp2-5BB-2 5.5 2.6 17.2 25.3 32.6 2.9 TF0076rp2-5BB-3 5.4 2.1 12.8 20.2 28.5 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0384] Table 4.19 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.19 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00148 TABLE 4.19 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TF0076rp2 1.16 1.11 0.95 1.04 1.07 1.02 1.02

[0385] Table 4.20 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00149 TABLE 4.20 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0076rp2 1.24 1.13 0.79 1.12 1.13

[0386] Table 4.21 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.21 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00150 TABLE 4.21 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0076rp2-3BB 1.22 1.27 1.17 1.18 0.92 1.06 TF0076rp2-4B 1.42 1.29 0.95 1.16 1.23 1.03 0.96 TF0076rp2-5BA 1.06 0.94 0.88 0.92 1.14 1.00 TF0076rp2-5BB 0.95 0.94 0.94 0.94 0.98 1.06

Construction Group TF0097

[0387] This construct induce increase of biomass production. Dry weight measurements of the construction group show 74% increase of average stem, 82% increase of average bark, 28% increase of average leaf and 43% increase of average TOTAL compared to the corresponding wildtype group. One of the construction group lines show 136% increase of average stem, 141% increase of average bark, 63% increase of average leaf and 87% increase of average TOTAL compared to the corresponding wildtype group. For the line were root dry weight were measured an increase in the shot-root ratio were observed.

[0388] Table 4.22 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00151 TABLE 4.22 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL(g) (m2/kg) (cm) TF0097rp1-1A-1 12.2 5.5 21.8 39.4 27.1 3.6 TF0097rp1-1A-2 11.1 5.3 18.3 34.7 28.6 3.8 TF0097rp1-1A-3 8.3 4.1 16.6 29.0 28.2 3.6 TF0097rp1-2A-1 9.1 3.3 21.9 34.3 35.5 2.6 TF0097rp1-2A-2 9.4 3.8 23.8 37.0 33.6 2.7 TF0097rp1-2A-3 8.2 3.2 21.0 32.4 30.6 2.7 TF0097rp1-2B-1 5.7 3.8 13.4 22.8 20.5 3.1 TF0097rp1-2B-2 10.5 5.3 21.6 37.4 25.2 3.8 TF0097rp1-2B-3 14.2 6.1 27.9 48.1 25.1 3.3 TF0097rp1-3A-1 15.3 7.2 28.9 51.4 28.5 3.6 TF0097rp1-3A-2 10.1 4.0 6.3 20.7 34.7 33.2 3.7 TF0097rp1-3A-3 14.6 6.6 11.3 28.2 49.4 27.0 3.6 TF0097rp1-4A-1 4.3 2.2 8.9 15.4 27.9 2.8 TF0097rp1-4A-2 5.1 2.5 13.1 20.6 28.7 3.5 TF0097rp1-4A-3 9.4 4.3 18.6 32.4 26.5 3.6 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0389] Table 4.23 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.23 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00152 TABLE 4.23 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TF0097rp1 1.74 1.82 1.07 1.28 1.43 0.83 1.25

[0390] Table 4.24 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00153 TABLE 4.24 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0097rp1 2.19 2.31 1.10 1.40 1.68

[0391] Table 4.25 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.25 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00154 TABLE 4.25 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0097rp1-1A 1.86 2.02 1.19 1.42 0.82 1.37 TF0097rp1-2A 1.58 1.41 1.40 1.43 0.97 1.00 TF0097rp1-2B 1.79 2.06 1.32 1.49 0.69 1.29 TF0097rp1-3A 2.36 2.41 1.07 1.63 1.87 0.86 1.36 TF0097rp1-4A 1.11 1.22 0.85 0.94 0.81 1.25

Construction Group TF0109

[0392] This construct induce increase of biomass production. Dry weight measurements of the construction group show 57% increase of average stem, 56% increase of average bark, 34% increase of average leaf and 40% increase of average TOTAL compared to the corresponding wildtype group. One of the construction group lines show 82% increase of average stem, 62% increase of average bark, 10% increase of average leaf and 31% increase of average TOTAL compared to the corresponding wildtype group. For the line were root dry weight were measured an increase in the shot-root ratio were observed.

[0393] Table 4.26 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00155 TABLE 4.26 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL (g) (m2/kg) (cm) TF0109rp1-2A-1 10.5 4.2 25.5 40.2 29.0 2.8 TF0109rp1-2A-2 7.5 3.2 19.0 29.7 31.9 3.0 TF0109rp1-2A-3 7.7 3.3 21.9 32.9 30.4 2.7 TF0109rp1-2B-1 8.6 4.1 6.2 16.3 29.1 29.9 4.0 TF0109rp1-2B-2 11.4 4.3 6.5 19.8 35.5 32.6 3.9 TF0109rp1-2B-3 10.9 3.5 7.5 16.3 30.7 36.2 3.1 TF0109rp1-3B-1 9.0 3.9 21.4 34.3 38.5 2.8 TF0109rp1-3B-2 8.1 3.6 19.8 31.5 34.1 2.8 TF0109rp1-3B-3 8.6 3.6 22.0 34.1 31.6 2.9 TF0109rp1-4A-1 8.0 3.9 24.1 36.0 27.5 3.3 TF0109rp1-4A-2 8.3 4.0 24.6 37.0 33.3 3.1 TF0109rp1-4A-3 7.8 4.3 24.2 36.3 27.3 3.1 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0394] Table 4.27 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.27 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00156 TABLE 4.27 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TF0109rp1 1.57 1.56 0.81 1.34 1.40 0.93 1.18

[0395] Table 4.28 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00157 TABLE 4.28 Construction Maximum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TF0109rp1 1.63 1.39 0.74 1.23 1.31

[0396] Table 4.29 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.29 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00158 TABLE 4.29 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TF0109rp1-2A 1.51 1.45 1.39 1.41 0.89 1.06 TF0109rp1-2B 1.82 1.62 0.81 1.10 1.31 0.96 1.39 TF0109rp1-3B 1.51 1.51 1.32 1.38 1.01 1.07 TF0109rp1-4A 1.42 1.66 1.53 1.50 0.86 1.19

Construction Group TFSTT019

[0397] This construct induce increase of biomass production. Dry weight measurements of one of the construction group lines show 19% increase of average stem, 12% increase of average bark, 10% increase of average leaf and 11% increase of average TOTAL compared to the corresponding wildtype group. This gene also gives an increased SLA in many lines, which in many cases are coupled to efficient growth.

[0398] Table 4.30 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00159 TABLE 4.30 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL (g) (m2/kg) (cm) TFSTT019rp1-2A-1 5.1 2.1 15.0 22.2 39.0 2.5 TFSTT019rp1-2A-2 18.7 18.7 35.4 2.7 TFSTT019rp1-2A-3 5.8 2.5 16.9 25.1 40.0 2.6 TFSTT019rp1-3A-1 6.3 2.6 18.6 27.5 41.1 2.6 TFSTT019rp1-3A-2 2.2 1.2 11.2 14.5 30.9 2.1 TFSTT019rp1-3A-3 9.0 3.4 22.9 35.3 33.1 2.7 TFSTT019rp1-4BA-1 5.9 2.4 15.7 24.0 35.5 2.6 TFSTT019rp1-4BA-2 7.6 3.1 19.3 29.9 35.8 2.6 TFSTT019rp1-4BA-3 TFSTT019rp1-4BB-1 6.0 2.5 15.9 24.4 40.4 2.6 TFSTT019rp1-4BB-2 5.7 2.4 17.6 25.6 40.0 2.2 TFSTT019rp1-4BB-3 6.0 2.3 16.4 24.7 39.9 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0399] Table 4.31 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.31 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00160 TABLE 4.31 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TFSTT019rp1 1.05 1.00 1.08 1.02 1.09 0.95

[0400] Table 4.32 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00161 TABLE 4.32 Maxi- Construction mum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TFSTT019rp1 1.28 1.10 1.11 1.15

[0401] Table 4.33 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.33 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00162 TABLE 4.33 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TFSTT019rp1-2A 0.97 0.93 1.06 0.91 1.11 0.98 TFSTT019rp1-3A 1.03 0.98 1.10 1.06 1.02 0.93 TFSTT019rp1-4BA 1.19 1.12 1.10 1.11 1.04 0.97 TFSTT019rp1-4BB 1.04 0.98 1.05 1.03 1.17 0.91

Construction Group TFSTT051

[0402] This construct induce increase of biomass production. Dry weight measurements of one of the construction group lines show 22% increase of average stem, 30% increase of average bark, 29% increase of average leaf and 26% increase of average TOTAL compared to the corresponding wildtype group.

[0403] Table 4.34 contains dry weight data for specified construction group and corresponding wild type group.

TABLE-US-00163 TABLE 4.34 Construction Group SLA Internode Individual Stem (g) Bark (g) Root (g) Leaf (g) TOTAL (g) (m2/kg) (cm) TFSTT051rp1-3B-1 4.6 2.4 12.4 19.4 24.6 2.1 TFSTT051rp1-3B-2 4.0 2.3 11.4 17.6 24.1 2.0 TFSTT051rp1-3B-3 4.6 2.6 13.1 20.3 22.5 2.0 TFSTT051rp1-4A-1 6.7 2.9 20.1 29.7 30.5 2.9 TFSTT051rp1-4A-2 6.1 2.9 18.5 27.4 33.3 2.8 TFSTT051rp1-4A-3 8.0 3.7 22.9 34.6 31.8 2.5 T89-01 5.1 2.2 13.1 20.4 39.9 2.7 T89-02 13.8 28.4 36.3 2.6 T89-03 6.8 2.7 17.1 26.6 37.8 2.6 T89-04 6.9 2.7 17.3 27.0 37.1 2.4 T89-05 5.7 2.5 16.6 24.9 31.8 2.6 T89-06 6.1 2.7 8.2 18.4 27.1 31.7 2.7 T89-07 5.8 2.5 9.2 15.9 24.2 31.7 2.6 T89-08 3.9 2.1 16.1 22.0 29.2 2.6 T89-09 6.9 3.1 20.7 30.7 30.3 2.5 T89-10 5.5 2.5 10.3 16.6 24.6 36.9 2.6 T89-11 6.9 3.0 17.9 27.8 34.0 3.0 T89-12 6.6 3.0 15.2 24.8 31.9 2.8 T89-13 5.9 2.2 15.0 23.1 39.6 2.4 T89-14 3.4 1.7 13.2 18.3 32.7 2.5 T89-15 4.6 2.0 14.9 21.6 33.4 2.7 T89-16 6.1 2.7 17.7 26.4 31.8 2.6 T89-17 4.4 2.1 15.9 22.4 31.5 2.6 T89-18 5.6 2.6 15.0 23.3 32.7 2.7 T89-19 6.2 2.7 18.4 27.3 33.6 2.5 T89-20 5.9 2.7 8.4 16.5 25.2 35.9 2.7 T89-21 6.9 2.8 17.8 27.5 39.0 2.6 T89-22 5.4 2.2 13.7 21.3 37.9 2.7 T89-23 7.0 2.9 17.7 27.5 33.3 2.9 T89-24 6.6 2.7 17.3 26.6 33.8 2.6 T89-25 6.5 2.8 7.4 17.2 26.5 32.4 2.8 T89-26 4.7 2.2 6.2 14.3 21.2 33.0 2.8 T89-27 5.4 2.4 16.1 23.9 31.3 2.5 T89-28 3.6 1.5 11.7 16.7 39.5 2.5 T89-29 3.1 1.3 9.9 14.4 36.3 3.0 T89-30 5.0 2.1 13.7 20.8 36.2 2.9 T89-31 7.0 2.9 17.2 27.2 32.7 2.7 T89-32 6.0 2.5 16.7 25.2 32.3 2.5

[0404] Table 4.35 contains the dry weight ratios of specified construction group relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.35 also show ratios of average SLA and average internode length of specified construction group relative to corresponding wildtype group.

TABLE-US-00164 TABLE 4.35 Construction Average Average Average Average Average Average Average Group Stem Bark Root Leaf TOTAL SLA Internode TFSTT051rp1 1.00 1.15 1.03 1.03 0.81 0.90

[0405] Table 4.36 contains the dry weight ratios for specified construction group relative to corresponding wildtype group for maximum stem, maximum bark, maximum root, maximum leaf and maximum TOTAL.

TABLE-US-00165 TABLE 4.36 Maxi- Construction mum Maximum Maximum Maximum Maximum Group Stem Bark Root Leaf TOTAL TFSTT051rp1 1.15 1.20 1.11 1.13

[0406] Table 4.37 contains dry weight ratios for specified construction group line relative to corresponding wildtype group of average stem, average bark, average root, average leaf and average TOTAL. Table 4.37 also show ratios of average SLA and average internode length of specified construction group line relative to corresponding wildtype group.

TABLE-US-00166 TABLE 4.37 Line Line Line Line Line Line Line Construction Average Average Average Average Average Average Average Group Line Stem Bark Root Leaf TOTAL SLA Internode TFSTT051rp1-3B 0.77 1.00 0.77 0.79 0.69 0.76 TFSTT051rp1-4A 1.22 1.30 1.29 1.26 0.93 1.04

Example 5

Density Measurement

[0407] A 5 cm long steam section (the segment between 36 cm and 41 cm from the soil) of each plant was stored in a freezer (-20° C.) after harvest. Samples subjected to density measurement were first defrosted and debarked and then the central core was removed. The weight (w) was measured using a balance and the volume (v) was determent using the principle of Archimedes, the wood samples were pushed (using a needle) into a baker (placed on a balance) with water and the increase in weight is equivalent to weight of the water pushed aside by the wood sample and since the density of water is (1 g/cm³) it is equivalent to the volume of the wood samples. The samples were then dried in oven for >48 h at 45° C. The dry weight (dw) were measured and the density (d) was calculated according to (1).

d=dw/v (1)

[0408] Samples for each construction are compared with wild type samples (T89) from the same cultivation round. Each construction must fulfil two criteria's to be seen as a construction group with altered density.

[0409] 1. Significant differences in average density according to a t-test (p-value<0.01). The t-test is two sided and assuming unequal variance.

[0410] 2. Two or more individuals outside (on the same side) a 95% confidence interval around the wild type population.

Summary Table Density

TABLE-US-00167

[0411] Average Average Average (KR)/ T-Test Samples Above Signifcant KR T89 Average (T89) 99% 95% conf int change TF0002Rp2 0.335 0.284 1.179 Sign 4 YES TF0003 0.291 0.255 1.140 Sign 2 YES TF0011 0.314 0.284 1.106 Sign 2 YES TF0013 0.252 0.260 0.970 Not sign 0 NO TF0045 0.306 0.274 1.117 Sign 2 YES TF0052 0.302 0.275 1.099 Not sign 2 NO TF0065 0.273 0.260 1.051 Not sign 1 NO TF0076 0.267 0.260 1.027 Not sign 0 NO TF0076Rp2 0.285 0.284 1.002 Not sign 1 NO TF0096 0.307 0.274 1.121 Not sign 1 NO TF0097Rp1 0.314 0.284 1.107 Sign 7 YES TF0104 0.284 0.274 1.036 Not sign 0 NO TF0109Rp1 0.286 0.284 1.007 Not sign 3 NO* TF0116 0.277 0.275 1.008 Not sign 0 NO TF0132.2nd 0.294 0.274 1.071 Not sign 1 NO TF0132rp1 0.272 0.255 1.068 Sign 4 YES TF0146 0.318 0.274 1.161 Sign 3 YES TF0173 0.256 0.254 1.008 Not sign 0 NO TF0247 0.294 0.274 1.073 Not sign 1 NO TF0405 0.287 0.274 1.045 Not sign 0 NO TFSTT004 0.284 0.275 1.033 Not sign 0 NO TFSTT013 0.319 0.284 1.123 Sign 2 YES TFSTT016 0.270 0.275 0.980 Not sign 0 NO TFSTT019 0.257 0.260 0.989 Not sign 0 NO TFSTT035 0.249 0.260 0.957 Not sign 0 NO TFSTT036 0.306 0.274 1.117 Sign 2 YES TFSTT038 0.295 0.275 1.073 Not sign 0 NO TFSTT045 0.274 0.274 1.000 Not sign 1 NO TFSTT051 0.278 0.275 1.013 Not sign 0 NO *The construction group TF0109 (Replant 1) does not fulfil the criteria for altered density, but the construction group lines; TF0109Rp1-2B (+18%) and TF0109Rp1-4A (-16%) do.

Explanation of Construction Group Summary Tables Density.

[0412] All densities are given in g/cm³

TABLE-US-00168 TFXXX Average TFXXX: Construction group average Max TFXXX: Construction group max Min TFXXX: Construction group min Number of TFXXX: Number of sample for the group construction Number of TFXXX lines: Number of lines (only shown when number of lines differs form number of samples) Average T89: Wild type average Max T89: Wild type max Min T89: Wild type min Number of T89: Number of wild type samples Confidence interval (95%) (Wild type mean) +/- (t_table(2 - tailed 95%) * Standard deviation for wild type samples) T-test T-test Number of TFXXX > CI upper limit Number TFXXX samples outside the confidence interval's upper limit Number of TFXXX < CI lower limit Number TFXXX samples outside the confidence interval's lower limit Average (TFXXX)/Average(T89) Construction group average/ Wild type average Max(TFXXX)/Max(T89) Construction group max/ Wild type max

[0413] The following construction groups have not generated any data TF0089, TF0097, TF0109, TF0132 and TFSTT047.

Construction Group TF0002 (Replant 2)

Raw Data TF002Rp2 Density

TABLE-US-00169

[0414] TF0002Rp2 Density Individual name: (g/cm³) TF0002Rp2-1B-1 0.317 TF0002Rp2-1B-2 0.360 TF0002Rp2-1B-3 0.323 TF0002Rp2-2A-1 0.322 TF0002Rp2-2A-2 0.323 TF0002Rp2-2A-3 0.366 TF0002Rp2-3B-1 0.330 TF0002Rp2-3B-2 0.321 TF0002Rp2-3B-3 0.352

Summary Density TF002Rp2

TABLE-US-00170

[0415] TF0002Rp2 Average TF0002Rp2: 0.335 Max TF0002Rp2: 0.366 Min TF0002Rp2: 0.317 Number of TF0002Rp2: 9 Number of TF0002Rp2 lines: 3 Average T89: 0.284 Max T89: 0.338 Min T89: 0.252 Number of T89: 32 Confidence interval (95%) 0.284 +/- 0.041 T-test 4.066E-08 Number of TF0002Rp2 > 0.325 4 Number of TF0002Rp2 < 0.243 0 Average (TF0002Rp2)/Average(T89) 1.179 Max(TF0002Rp2)/Max(T89) 1.083

[0416] TF0002Rp2 has significant higher density (according to criteria 1 and 2) (+18% in average) than corresponding T89 group. The density change (compared to T89) for the 3 construction group lines of TF0002Rp2 (3 individuals of each line), TF0002Rp2-1B (+17% in average), TF0002Rp2-2A (+19% in average) and TF0002Rp2-3B (+18 in average). Line TF0002Rp2-3B itself fulfils the criteria 1 and 2.

Construction Group TF0003

Raw Data TF0003 Density

TABLE-US-00171

[0417] TF0003 Density Individual name: (g/cm³) TF0003-1A 0.318 TF0003-1B 0.323 TF0003-2A 0.242 TF0003-3A TF0003-3B TF0003-4A TF0003-4B 0.282

Summary Density TF0003

TABLE-US-00172

[0418] TF0003 Average TF0003: 0.291 Max TF0003: 0.323 Min TF0003: 0.242 Number of TF0003: 4 Number of TF0003 lines: Average T89: 0.255 Max T89: 0.313 Min T89: 0.221 Number of T89: 39 Confidence interval (95%) 0.255 +/- 0.045 T-test 6.712E-03 Number of TF0003 > 0.301 2 Number of TF0003 < 0.21 0 Average (TF0003)/Average(T89) 1.140 Max(TF0003)/Max(T89) 1.032

[0419] Construction group TF0003 has significant higher density (according to criteria 1 and 2) (+14% in average) than corresponding T89 group.

Construction Group TF0011

Raw Data TF0011 Density

TABLE-US-00173

[0420] TF0011 Density Individual name: (g/cm³) TF0011-1A-1 0.308 TF0011-1A-2 0.360 TF0011-1B 0.264 TF0011-2A-1 0.337 TF0011-2A-2 0.298 TF0011-3A-1 0.326 TF0011-3A-2 0.350 TF0011-3B-1 0.333 TF0011-3B-2 0.303 TF0011-4A 0.261

Summary Density TF0011

TABLE-US-00174

[0421] TF0011 Average TF0011: 0.314 Max TF0011: 0.360 Min TF0011: 0.261 Number of TF0011: 10 Number of TF0011 lines: Average T89: 0.284 Max T89: 0.361 Min T89: 0.222 Number of T89: 41 Confidence interval (95%) 0.284 +/- 0.06 T-test 7.377E-03 Number of TF0011 > 0.344 2 Number of TF0011 < 0.224 0 Average (TF0011)/Average(T89) 1.106 Max(TF0011)/Max(T89) 0.997

[0422] Construction group TF0011 has significant higher density (according to criteria 1 and 2) (+11% in average) than corresponding T89 group.

Construction Group TF0013

Raw Data TF0013 Density

TABLE-US-00175

[0423] TF0013 Density Individual name: (g/cm³) TF0013-1A-1 0.246 TF0013-1A-2 0.236 TF0013-2A TF0013-2B 0.265 TF0013-3A 0.258 TF0013-3BA 0.254 TF0013-3BB 0.245 TF0013-4BA 0.258 TF0013-4BB 0.253

Summary Density TF0013

TABLE-US-00176

[0424] TF0013 Average TF0013: 0.252 Max TF0013: 0.265 Min TF0013: 0.236 Number of TF0013: 8 Number of TF0013 lines: Average T89: 0.260 Max T89: 0.358 Min T89: 0.218 Number of T89: 45 Confidence interval (95%) 0.26 +/- 0.049 T-test 3.760E-01 Number of TF0013 > 0.309 0 Number of TF0013 < 0.211 0 Average (TF0013)/Average(T89) 0.970 Max(TF0013)/Max(T89) 0.740

[0425] Construction group TF0013 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0045

Raw Data TF0045 Density

TABLE-US-00177

[0426] TF0045 Density Individual name: (g/cm³) TF0045-1A-1 0.344 TF0045-1A-2 TF0045-1A-3 0.270 TF0045-1B-1 0.320 TF0045-1B-2 0.323 TF0045-1B-3 0.328 TF0045-2B-1 0.303 TF0045-2B-3 0.255

Summary Density TF0045

TABLE-US-00178

[0427] TF0045 Average TF0045: 0.306 Max TF0045: 0.344 Min TF0045: 0.255 Number of TF0045: 7 Number of TF0045 lines: 3 Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 5.760E-03 Number of TF0045 > 0.326 2 Number of TF0045 < 0.222 0 Average (TF0045)/Average(T89) 1.117 Max(TF0045)/Max(T89) 0.972

[0428] TF0045 has significant higher density (according to criteria 1 and 2) (+12% in average) than corresponding T89 group. The density change (compared to T89) for the 3 construction group lines of TF0045, TF0045-1A (+12% in average (2 measured individuals)), TF0045-1B (+18% in average (3 individuals)) and TF0045-2B (+2% in average (2 individuals)).

Construction Group TF0052

Raw Data TF0052 Density

TABLE-US-00179

[0429] TF0052 Density Individual name: (g/cm³) TF0052-1A 0.241 TF0052-1B 0.346 TF0052-2A 0.323 TF0052-2B 0.251 TF0052-3A 0.393 TF0052-3B 0.278 TF0052-4A 0.261 TF0052-4B 0.322

Summary Density TF0052

TABLE-US-00180

[0430] TF0052 Average TF0052: 0.302 Max TF0052: 0.393 Min TF0052: 0.241 Number of TF0052: 8 Number of TF0052 lines: Average T89: 0.275 Max T89: 0.345 Min T89: 0.223 Number of T89: 23 Confidence interval (95%) 0.275 +/- 0.069 T-test 9.876E-02 Number of TF0052 > 0.343 2 Number of TF0052 < 0.206 0 Average (TF0052)/Average(T89) 1.099 Max(TF0052)/Max(T89) 1.139

[0431] Construction group TF0052 has no significant difference in density (according to criteria 1) compared with corresponding T89 group. Although TF0052 has an increased density (+10%) in average and fulfil criteria 2.

Construction Group TF0065

Raw Data TF0065 Density

TABLE-US-00181

[0432] TF0065 Density Individual name: (g/cm³) TF0065-1AA 0.333 TF0065-1AB 0.245 TF0065-1BA 0.267 TF0065-1BB 0.253 TF0065-2B 0.278 TF0065-3A 0.262 TF0065-4B 0.272

Summary Density TF0065

TABLE-US-00182

[0433] TF0065 Average TF0065: 0.273 Max TF0065: 0.333 Min TF0065: 0.245 Number of TF0065: 7 Number of TF0065 lines: Average T89: 0.260 Max T89: 0.358 Min T89: 0.218 Number of T89: 45 Confidence interval (95%) 0.26 +/- 0.049 T-test 2.012E-01 Number of TF0065 > 0.309 1 Number of TF0065 < 0.211 0 Average (TF0065)/Average(T89) 1.051 Max(TF0065)/Max(T89) 0.931

[0434] Construction group TF0065 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0076

Raw Data TF0076 Density

TABLE-US-00183

[0435] TF0076 Density Individual name: (g/cm³) TF0076-2AA 0.250 TF0076-2AB 0.253 TF0076-3BA 0.252 TF0076-3BB 0.257 TF0076-4B TF0076-5BA 0.288 TF0076-5BB 0.300

Summary Density TF0065

TABLE-US-00184

[0436] TF0076 Average TF0076: 0.267 Max TF0076: 0.300 Min TF0076: 0.250 Number of TF0076: 6 Number of TF0076 lines: Average T89: 0.260 Max T89: 0.358 Min T89: 0.218 Number of T89: 45 Confidence interval (95%) 0.26 +/- 0.049 T-test 5.054E-01 Number of TF0076 > 0.309 0 Number of TF0076 < 0.211 0 Average (TF0076)/Average(T89) 1.027 Max(TF0076)/Max(T89) 0.839

[0437] Construction group TF0076 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0076 (Replant 2)

Raw Data TF0076Rp2 Density

TABLE-US-00185

[0438] TF0076Rp2 Density Individual name: (g/cm³) TF0076Rp2-3BB-1 0.237 TF0076Rp2-3BB-2 0.311 TF0076Rp2-3BB-3 0.274 TF0076Rp2-4B-1 0.304 TF0076Rp2-4B-2 0.293 TF0076Rp2-4B-3 0.301 TF0076Rp2-5BA-1 0.262 TF0076Rp2-5BA-2 0.288 TF0076Rp2-5BA-3 0.259 TF0076Rp2-5BB-1 0.267 TF0076Rp2-5BB-2 0.263 TF0076Rp2-5BB-3 0.356

Summary Density TF0076Rp2

TABLE-US-00186

[0439] TF0076Rp2 Average TF0076Rp2: 0.285 Max TF0076Rp2: 0.356 Min TF0076Rp2: 0.237 Number of TF0076Rp2: 12 Number of TF0076Rp2 lines: 4 Average T89: 0.284 Max T89: 0.338 Min T89: 0.252 Number of T89: 32 Confidence interval (95%) 0.284 +/- 0.041 T-test 9.407E-01 Number of TF0076Rp2 > 0.325 1 Number of TF0076Rp2 < 0.243 1 Average (TF0076Rp2)/Average(T89) 1.002 Max(TF0076Rp2)/Max(T89) 1.054

[0440] Construction group TF0076Rp2 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0096

Raw Data TF0096 Density

TABLE-US-00187

[0441] TF0096 Density Individual name: (g/cm³) TF0096-2A 0.296 TF0096-2B 0.249 TF0096-3A 0.321 TF0096-3B 0.308 TF0096-4A 0.363

Summary Density TF0096

TABLE-US-00188

[0442] TF0096 Average TF0096: 0.307 Max TF0096: 0.363 Min TF0096: 0.249 Number of TF0096: 5 Number of TF0096 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 1.562E-02 Number of TF0096 > 0.326 1 Number of TF0096 < 0.222 0 Average (TF0096)/Average(T89) 1.121 Max(TF0096)/Max(T89) 1.024

[0443] Construction group TF0096 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0097 (Replant 1)

Raw Data TF0097Rp1 Density

TABLE-US-00189

[0444] TF0097Rp1 Density Individual name: (g/cm³) TF0097Rp1-1A-1 0.327 TF0097Rp1-1A-2 0.335 TF0097Rp1-1A-3 0.344 TF0097Rp1-2A-1 0.275 TF0097Rp1-2A-2 0.284 TF0097Rp1-2A-3 0.308 TF0097Rp1-2B-1 0.328 TF0097Rp1-2B-2 0.351 TF0097Rp1-2B-3 0.309 TF0097Rp1-3A-1 0.309 TF0097Rp1-3A-2 0.268 TF0097Rp1-3A-3 0.297 TF0097Rp1-4A-1 0.333 TF0097Rp1-4A-2 0.325 TF0097Rp1-4A-3 0.319

Summary Density TF0097Rp1

TABLE-US-00190

[0445] TF0097Rp1 Average TF0097Rp1: 0.314 Max TF0097Rp1: 0.351 Min TF0097Rp1: 0.268 Number of TF0097Rp1: 15 Number of TF0097Rp1 lines: 5 Average T89: 0.284 Max T89: 0.338 Min T89: 0.252 Number of T89: 32 Confidence interval (95%) 0.284 +/- 0.041 T-test 4.607E-05 Number of TF0097Rp1 > 0.325 7 Number of TF0097Rp1 < 0.243 0 Average (TF0097Rp1)/Average(T89) 1.107 Max(TF0097Rp1)/Max(T89) 1.038

[0446] TF0097Rp1 has significant higher density (according to criteria 1 and 2) (+11% in average) than corresponding T89 group. The density change (compared to T89) for the 5 construction group lines of TF0097Rp1 (3 individuals of each line), TF00097Rp1-1A (+18% in average), TF00097Rp1-2A (+2% in average), TF00097Rp1-2B (+16% in average), TF00097Rp1-3A (+3% in average) and TF00097Rp1-4A (+15 in average). The lines TF00097Rp1-1A, TF00097Rp1-2B and TF00097Rp1-4A them self fulfil criteria 1 and 2.

Construction Group TF0104

Raw Data TF0104 Density

TABLE-US-00191

[0447] TF0104 Density Individual name: (g/cm³) TF0104-1A 0.282 TF0104-1B 0.282 TF0104-2A 0.297 TF0104-3A 0.298 TF0104-3B 0.261

Summary Density TF0104

TABLE-US-00192

[0448] TF0104 Average TF0104: 0.284 Max TF0104: 0.298 Min TF0104: 0.261 Number of TF0104: 5 Number of TF0104 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 4.063E-01 Number of TF0104 > 0.326 0 Number of TF0104 < 0.222 0 Average (TF0104)/Average(T89) 1.036 Max(TF0104)/Max(T89) 0.842

[0449] Construction group TF0104 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0109 (Replant 1)

Raw Data TF0109Rp1 Density

TABLE-US-00193

[0450] TF0109Rp1 Density Individual name: (g/cm³) TF0109Rp1-2A-1 0.301 TF0109Rp1-2A-2 0.294 TF0109Rp1-2A-3 0.284 TF0109Rp1-2B-1 0.329 TF0109Rp1-2B-2 0.342 TF0109Rp1-2B-3 0.336 TF0109Rp1-3B-1 0.279 TF0109Rp1-3B-2 0.279 TF0109Rp1-3B-3 0.271 TF0109Rp1-4A-1 0.253 TF0109Rp1-4A-2 0.238 TF0109Rp1-4A-3 0.222

Summary Density TF0109Rp1

TABLE-US-00194

[0451] TF0109Rp1 Average TF0109Rp1: 0.286 Max TF0109Rp1: 0.342 Min TF0109Rp1: 0.222 Number of TF0109Rp1: 12 Number of TF0109Rp1 lines: 4 Average T89: 0.284 Max T89: 0.338 Min T89: 0.252 Number of T89: 32 Confidence interval (95%) 0.284 +/- 0.041 T-test 8.314E-01 Number of TF0109Rp1 > 0.325 3 Number of TF0109Rp1 < 0.243 2 Average (TF0109Rp1)/Average(T89) 1.007 Max(TF0109Rp1)/Max(T89) 1.014

[0452] Construction group TF0109Rp1 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group. The density change (compared to T89) for the 4 construction group lines of TF0109Rp1 (3 individuals of each line), TF0109Rp1-2A (+3% in average), TF0109Rp1-2B (+18% in average), TF0109Rp1-3B (-3% in average and TF0109Rp1-4A (-16 in average). The lines TF0109Rp1-2B and TF0109Rp1-4A them self fulfil criteria 1 and 2.

Construction Group TF0116

Raw Data TF0116 Density

TABLE-US-00195

[0453] TF0116 Density Individual name: (g/cm³) TF0116-1B TF0116-2A 0.306 TF0116-2B-1 0.293 TF0116-2B-2 0.263 TF0116-4A 0.292 TF0116-5B 0.247 TF0116-6A 0.271 TF0116-6B 0.268

Summary Density TF0116

TABLE-US-00196

[0454] TF0116 Average TF0116: 0.277 Max TF0116: 0.306 Min TF0116: 0.247 Number of TF0116: 7 Number of TF0116 lines: Average T89: 0.275 Max T89: 0.345 Min T89: 0.223 Number of T89: 23 Confidence interval (95%) 0.275 +/- 0.069 T-test 8.725E-01 Number of TF0116 > 0.343 0 Number of TF0116 < 0.206 0 Average (TF0116)/Average(T89) 1.008 Max(TF0116)/Max(T89) 0.887

[0455] Construction group TF0116 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0132 (2nd Set of Construction Group Lines)

Raw Data TF0132.2nd Density

TABLE-US-00197

[0456] TF0132.2nd Density Individual name: (g/cm³) TF0132.2nd-1A 0.263 TF0132.2nd-1B 0.284 TF0132.2nd-2A 0.350 TF0132.2nd-4B 0.315 TF0132.2nd-5A 0.271 TF0132.2nd-5B 0.286 TF0132.2nd-6B 0.285 TF0132.2nd-7A 0.294

Summary Density TF0132.2nd

TABLE-US-00198

[0457] TF0132.2nd Average TF0132.2nd: 0.294 Max TF0132.2nd: 0.350 Min TF0132.2nd: 0.263 Number of TF0132.2nd: 8 Number of TF0132.2nd lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 6.257E-02 Number of TF0132.2nd > 0.326 1 Number of TF0132.2nd < 0.222 0 Average (TF0132.2n)/Average(T89) 1.071 Max(TF0132.2nd)/Max(T89) 0.988

[0458] Construction group TF0132.2nd has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0132 (Replant 1)

Raw Data TF0132rp1 Density

TABLE-US-00199

[0459] TF0132rp1 Density Individual name: (g/cm³) TF0132Rp1-1B-1 0.274 TF0132Rp1-1B-2 0.265 TF0132Rp1-1B-3 0.265 TF0132Rp1-3BB-1 0.260 TF0132Rp1-3BB-2 0.265 TF0132Rp1-3BB-3 0.267 TF0132Rp1-4AC-1 0.283 TF0132Rp1-4AC-2 0.275 TF0132Rp1-4AC-3 0.278 TF0132Rp1-4B-1 0.291 TF0132Rp1-4B-2 0.260 TF0132Rp1-4B-3 0.282 TF0132Rp1-6B-1 0.270 TF0132Rp1-6B-2 0.281 TF0132Rp1-6B-3 0.269

Summary Density TF0132Rp1

TABLE-US-00200

[0460] TF0132rp1 Average TF0132rp1: 0.272 Max TF0132rp1: 0.291 Min TF0132rp1: 0.260 Number of TF0132rp1: 15 Number of TF0132rp1 lines: 5 Average T89: 0.255 Max T89: 0.275 Min T89: 0.232 Number of T89: 27 Confidence interval (95%) 0.255 +/- 0.025 T-test 2.603E-05 Number of TF0132rp1 > 0.280 4 Number of TF0132rp1 < 0.230 0 Average (TF0132rp1)/Average(T89) 1.068 Max(TF0132rp1)/Max(T89) 1.059

[0461] Construction group TF0132Rp1 has significant higher density (according to criteria 1 and 2) (+7% in average) than corresponding T89 group. The density change (compared to T89) for the 5 construction group lines of TF0132Rp1 (3 individuals of each line), T TF0132Rp1-1B (+5% in average), TF0132Rp1-3BB (+4% in average), TF0132Rp1-4AC (+9% in average), TF0132Rp1-4B (+9% in average) and TF0132Rp1-6B (+7% in average). The line TF0132Rp1-4B itself fulfil criteria 1 and 2.

Construction Group TF0146

Raw Data TF0146 Density

TABLE-US-00201

[0462] TF0146 Density Individual name: (g/cm³) TF0146-1A 0.341 TF0146-1B 0.314 TF0146-2A 0.303 TF0146-2B 0.300 TF0146-3A 0.333 TF0146-3B 0.313 TF0146-4A 0.374 TF0146-4B 0.269

Summary Density TF0146

TABLE-US-00202

[0463] TF0146 Average TF0146: 0.318 Max TF0146: 0.374 Min TF0146: 0.269 Number of TF0146: 8 Number of TF0146 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 1.127E-04 Number of TF0146 > 0.326 3 Number of TF0146 < 0.222 0 Average (TF0146)/Average(T89) 1.161 Max(TF0146)/Max(T89) 1.054

[0464] Construction group TF0146 has significant higher density (according to criteria 1 and 2) (+16% in average) than corresponding T89 group.

Construction Group TF0173

Raw Data TF0173 Density

TABLE-US-00203

[0465] TF0173 Density Individual name: (g/cm³) TF0173-3A-1 0.232 TF0173-3A-2 0.291 TF0173-3B-1 0.239 TF0173-3B-2 0.242 TF0173-4A-1 0.251 TF0173-4A-2 0.231 TF0173-4B-1 0.256 TF0173-4B-2 0.305

Summary Density TF0173

TABLE-US-00204

[0466] TF0173 Average TF0173: 0.256 Max TF0173: 0.305 Min TF0173: 0.231 Number of TF0173: 8 Number of TF0173 lines: Average T89: 0.254 Max T89: 0.345 Min T89: 0.221 Number of T89: 34 Confidence interval (95%) 0.254 +/- 0.056 T-test 8.478E-01 Number of TF0173 > 0.31 0 Number of TF0173 < 0.198 0 Average (TF0173)/Average(T89) 1.008 Max(TF0173)/Max(T89) 0.884

[0467] Construction group TF0173 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0247

Raw Data TF0247 Density

TABLE-US-00205

[0468] TF0247 Density Individual name: (g/cm³) TF0247-1A 0.257 TF0247-3A 0.250 TF0247-3B 0.309 TF0247-4A 0.288 TF0247-6B 0.366

Summary Density TF0247

TABLE-US-00206

[0469] TF0247 Average TF0247: 0.294 Max TF0247: 0.366 Min TF0247: 0.250 Number of TF0247: 5 Number of TF0247 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 1.498E-01 Number of TF0247 > 0.326 1 Number of TF0247 < 0.222 0 Average (TF0247)/Average(T89) 1.073 Max(TF0247)/Max(T89) 1.032

[0470] Construction group TF0247 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TF0405

Raw Data TF0405 Density

TABLE-US-00207

[0471] TF0405 Density Individual name: (g/cm³) TF0405-2A-1 0.271 TF0405-2A-2 0.291 TF0405-2B-2 TF0405-3A-1 0.275 TF0405-3A-2 0.309 TF0405-3B-1 0.295 TF0405-3B-2 0.279

Summary Density TF0405

TABLE-US-00208

[0472] TF0405 Average TF0405: 0.287 Max TF0405: 0.309 Min TF0405: 0.271 Number of TF0405: 6 Number of TF0405 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 2.560E-01 Number of TF0405 > 0.326 0 Number of TF0405 < 0.222 0 Average (TF0405)/Average(T89) 1.045 Max(TF0405)/Max(T89) 0.871

[0473] Construction group TF0405 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT004

Raw Data TFSTT004 Density

TABLE-US-00209

[0474] TFSTT004 Density Individual name: (g/cm³) TFSTT004-1A 0.300 TFSTT004-2A-1 0.285 TFSTT004-2A-2 0.283 TFSTT004-2B-1 0.272 TFSTT004-2B-2 0.262 TFSTT004-3B 0.329 TFSTT004-4B-1 0.266 TFSTT004-4B-2 0.274

Summary Density TFSTT004

TABLE-US-00210

[0475] TFSTT004 Average TFSTT004: 0.284 Max TFSTT004: 0.329 Min TFSTT004: 0.262 Number of TFSTT004: 8 Number of TFSTT004 lines: Average T89: 0.275 Max T89: 0.345 Min T89: 0.223 Number of T89: 23 Confidence interval (95%) 0.275 +/- 0.069 T-test 4.777E-01 Number of TFSTT004 > 0.343 0 Number of TFSTT004 < 0.206 0 Average (TFSTT004)/Average(T89) 1.033 Max(TFSTT004)/Max(T89) 0.955

[0476] Construction group TFSTT004 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT013

Raw Data TFSTT013 Density

TABLE-US-00211

[0477] TFSTT013 Density Individual name: (g/cm³) TFSTT013-1A 0.323 TFSTT013-1B 0.328 TFSTT013-2B 0.288 TFSTT013-3A 0.345 TFSTT013-3B 0.291 TFSTT013-4A 0.304 TFSTT013-4B 0.324 TFSTT013-5B 0.346

Summary Density TFSTT013

TABLE-US-00212

[0478] TFSTT013 Average TFSTT013: 0.319 Max TFSTT013: 0.346 Min TFSTT013: 0.288 Number of TFSTT013: 8 Number of TFSTT013 lines: Average T89: 0.284 Max T89: 0.361 Min T89: 0.222 Number of T89: 41 Confidence interval (95%) 0.284 +/- 0.06 T-test 3.006E-03 Number of TFSTT013 > 0.344 2 Number of TFSTT013 < 0.224 0 Average (TFSTT013)/Average(T89) 1.123 Max(TFSTT013)/Max(T89) 0.958

[0479] Construction group TFSTT013 has significant higher density (according to criteria 1 and 2) (+12% in average) than corresponding T89 group.

Construction Group TFSTT016

Raw Data TFSTT016 Density

TABLE-US-00213

[0480] TFSTT016 Density Individual name: (g/cm³) TFSTT016-1A 0.306 TFSTT016-1B 0.254 TFSTT016-2A 0.249 TFSTT016-2B 0.279 TFSTT016-3A-1 0.272 TFSTT016-3A-2 0.274 TFSTT016-4A 0.252

Summary Density TFSTT016

TABLE-US-00214

[0481] TFSTT016 Average TFSTT016: 0.270 Max TFSTT016: 0.306 Min TFSTT016: 0.249 Number of TFSTT016: 7 Number of TFSTT016 lines: Average T89: 0.275 Max T89: 0.345 Min T89: 0.223 Number of T89: 23 Confidence interval (95%) 0.275 +/- 0.069 T-test 6.884E-01 Number of TFSTT016 >0.343 0 Number of TFSTT016 <0.206 0 Average (TFSTT016)/Average (T89) 0.980 Max (TFSTT016)/Max (T89) 0.886

[0482] Construction group TFSTT016 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT019

Raw Data TFSTT019 Density

TABLE-US-00215

[0483] TFSTT019 Density Individual name: (g/cm³) TFSTT019-1A TFSTT019-1BA 0.216 TFSTT019-1BB 0.283 TFSTT019-2A 0.265 TFSTT019-2B 0.231 TFSTT019-3A 0.277 TFSTT019-4BA 0.244 TFSTT019-4BB 0.282

Summary Density TFSTT019

TABLE-US-00216

[0484] TFSTT019 Average TFSTT019: 0.257 Max TFSTT019: 0.283 Min TFSTT019: 0.216 Number of TFSTT019: 7 Number of TFSTT019 lines: Average T89: 0.260 Max T89: 0.358 Min T89: 0.218 Number of T89: 45 Confidence interval (95%) 0.26 +/- 0.049 T-test 7.862E-01 Number of TFSTT019 >0.309 0 Number of TFSTT019 <0.211 0 Average (TFSTT019)/Average (T89) 0.989 Max (TFSTT019)/Max (T89) 0.789

[0485] Construction group TFSTT019 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT035

[0486] Raw Data TFSTT035density

TABLE-US-00217 TFSTT035 Density Individual name: (g/cm³) TFSTT035-1A 0.236 TFSTT035-1BA 0.260 TFSTT035-1BB 0.265 TFSTT035-2AA 0.247 TFSTT035-2AB 0.257 TFSTT035-2B 0.217 TFSTT035-3B 0.240 TFSTT035-4B 0.267

Summary Density TFSTT035

TABLE-US-00218

[0487] TFSTT035 Average TFSTT035: 0.249 Max TFSTT035: 0.267 Min TFSTT035: 0.217 Number of TFSTT035: 8 Number of TFSTT035 lines: Average T89: 0.260 Max T89: 0.358 Min T89: 0.218 Number of T89: 45 Confidence interval (95%) 0.26 +/- 0.049 T-test 2.247E-01 Number of TFSTT035 >0.309 0 Number of TFSTT035 <0.211 0 Average (TFSTT035)/Average (T89) 0.957 Max (TFSTT035)/Max (T89) 0.745

[0488] Construction group TFSTT035 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT036

Raw Data TFSTT036 Density

TABLE-US-00219

[0489] TFSTT036 Density Individual name: (g/cm³) TFSTT036-1B 0.291 TFSTT036-2A 0.306 TFSTT036-2B 0.365 TFSTT036-3A 0.322 TFSTT036-4A 0.274 TFSTT036-4B 0.331 TFSTT036-5B 0.254

Summary Density TFSTT036

TABLE-US-00220

[0490] TFSTT036 Average TFSTT036: 0.306 Max TFSTT036: 0.365 Min TFSTT036: 0.254 Number of TFSTT036: 7 Number of TFSTT036 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 7.640E-03 Number of TFSTT036 >0.326 2 Number of TFSTT036 <0.222 0 Average (TFSTT036)/Average (T89) 1.117 Max (TFSTT036)/Max (T89) 1.030

[0491] Construction group TFSTT036 has significant higher density (according to criteria 1 and 2) (+12% in average) than corresponding T89 group.

Construction Group TFSTT038

[0492] Raw Data TFSTT038density

TABLE-US-00221 TFSTT038 Density Individual name: (g/cm³) TFSTT038-1A-1 0.256 TFSTT038-1A-2 0.292 TFSTT038-1B 0.312 TFSTT038-2A 0.308 TFSTT038-2B 0.264 TFSTT038-3A 0.301 TFSTT038-3B 0.343 TFSTT038-4B 0.284

Summary Density TFSTT038

TABLE-US-00222

[0493] TFSTT038 Average TFSTT038: 0.295 Max TFSTT038: 0.343 Min TFSTT038: 0.256 Number of TFSTT038: 8 Number of TFSTT038 lines: Average T89: 0.275 Max T89: 0.345 Min T89: 0.223 Number of T89: 23 Confidence interval (95%) 0.275 +/- 0.069 T-test 1.343E-01 Number of TFSTT038 >0.343 0 Number of TFSTT038 <0.206 0 Average (TFSTT038)/Average (T89) 1.073 Max (TFSTT038)/Max (T89) 0.995

[0494] Construction group TFSTT038 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT045

Raw Data TFSTT045 Density

TABLE-US-00223

[0495] TFSTT045 Density Individual name: (g/cm³) TFSTT045-1B 0.231 TFSTT045-2B 0.243 TFSTT045-3A 0.258 TFSTT045-3B 0.257 TFSTT045-4A 0.258 TFSTT045-4B 0.349 TFSTT045-7B 0.324

Summary Density TFSTT045

TABLE-US-00224

[0496] TFSTT045 Average TFSTT045: 0.274 Max TFSTT045: 0.349 Min TFSTT045: 0.231 Number of TFSTT045: 7 Number of TFSTT045 lines: Average T89: 0.274 Max T89: 0.354 Min T89: 0.226 Number of T89: 36 Confidence interval (95%) 0.274 +/- 0.052 T-test 9.934E-01 Number of TFSTT045 >0.326 1 Number of TFSTT045 <0.222 0 Average (TFSTT045)/Average (T89) 1.000 Max (TFSTT045)/Max (T89) 0.984

[0497] Construction group TFSTT045 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Construction Group TFSTT051

Raw Data TFSTT051 Density

TABLE-US-00225

[0498] TFSTT051 Density Individual name: (g/cm³) TFSTT051-1B 0.315 TFSTT051-2A 0.318 TFSTT051-2B 0.292 TFSTT051-3A 0.289 TFSTT051-3B 0.270 TFSTT051-4A 0.240 TFSTT051-4B-1 0.269 TFSTT051-4B-2 0.234

Summary Density TFSTT051

TABLE-US-00226

[0499] TFSTT051 Average TFSTT051: 0.278 Max TFSTT051: 0.318 Min TFSTT051: 0.234 Number of TFSTT051: 8 Number of TFSTT051 lines: Average T89: 0.275 Max T89: 0.345 Min T89: 0.223 Number of T89: 23 Confidence interval (95%) 0.275 +/- 0.069 T-test 7.969E-01 Number of TFSTT051 >0.343 0 Number of TFSTT051 <0.206 0 Average (TFSTT051)/Average (T89) 1.013 Max (TFSTT051)/Max (T89) 0.921

[0500] Construction group TFSTT051 has no significant difference in density (according to criteria 1 and 2) compared with corresponding T89 group.

Example 6

Fibre Measurements

[0501] Fibre measurements were performed on samples at 33 to 36 cm height of the stem. A piece of pure wood, about 1.5 mm×1.5 mm×15 mm was cut out from the stem piece. A maceration preparation (Franklin et al. 1945) was performed to get a macerate of single fibres from the small piece of wood. The sample was then measured using a KajaaniFibreLab® from Metso Automation, giving the average of fibre length, average fibre width and an estimation of the fibre cell wall thickness. The supplied computer software calculates these numbers using the below formulas according to the manufacturer.

Fibre Length

[0502] Average of fibre length, L(n), using true length of fibres, measured along the centreline:

L ( n ) = ( n i * l i ) n i [ mm ] ##EQU00003##

where n_i=number of fibres in class i, i=1 . . . 152, l_i=(0.05*i)-0.025, l_i=length of class i,

Fibre Width

[0503] Average of fibre width, W; based on cross sectional measurements:

W = ( n i * w i ) n i [ μm ] ##EQU00004##

where n_i=number of fibres in class i, i=1 . . . 100, w_i=kw*(i-0.5),

[0504] w_i=width of class i,

[0505] kw=width calibration factor,

Cell Wall Thickness

[0506] Average of cell wall thickness, CWT, based on cross sectional measurements:

CWT = ( n i * CWT i ) n i [ μm ] ##EQU00005##

where n_i=number of fibres in class i, i=1 . . . 100, CWT_i=kt*(i-0.5), CWT_i=cell wall thickness of class i, kt=cell wall thickness calibration factor.

[0507] Construction Groups with fibres with at least an 10% increase or 15% decrease in fibre length or widths were selected as being effected in genes useful for modifying fibre dimension according to the selection criteria's below.

Fibre Parameters Selection Criteria

[0508] In Table 6.1 the abbreviations used for the phenotypes used for the fibre selection criteria are listed.

TABLE-US-00227 TABLE 6.1 Abbreviations for phenotypes AFL average fibre length of the wild type population and each Construction group population AFW average fibre width of the wild type population and each Construction group population maxFL maximum fibre length of the wild type population and each Construction group population maxFW maximum fibre width of the wild type population and each Construction group population minFL minimum fibre length of the wild type population and each Construction group population minFW minimum fibre width of the wild type population and each Construction group population

[0509] Construction groups that showed a difference compared to the wild type population in any of the fibre parameters mentioned above were scored as construction groups that are altered in their growth properties and therefore the corresponding genes can be used to alter these properties.

[0510] As a 10% increase or a 15% decrease in fibre dimensions are of interest for the industry, the selection criteria below were used to select genes that can be used to altered fibre dimensions.

[0511] The fibre parameters selection criteria are as follows:

[0512] 1. If construction group AFL is at least 10% higher than corresponding wildtype group AFL, or

[0513] 2. If construction group AFW is at least 10% higher than corresponding wildtype group AFW, or

[0514] 3. If construction group maxFL is at least 10% higher than corresponding wildtype group maxFL, or

[0515] 4. If construction group maxFW is at least 10% higher than corresponding wildtype group maxFW, or

[0516] 5. If construction group AFL is at least 15% lower than corresponding wildtype group AFL, or

[0517] 6. If construction group AFW is at least 15% lower than corresponding wildtype group AFW, or

[0518] 7. If construction group minFL is at least 15% lower than corresponding wildtype group minFL, or

[0519] 8. If construction group minFW is at least 15% lower than corresponding wildtype group minFW.

[0520] Construction groups meeting one or more of these criteria were selected.

[0521] The results below are presented according to construction group.

Construction Group TF0002

[0522] This construct induces changes in fibre parameters. The maximum fibre width is 16% higher than corresponding maximum wildtype. The TF0002 construction group meets the fibre parameters selection criterion (4).

[0523] Table 6.2 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00228 TABLE 6.2 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TF0002-1B 0.37 17.73 4.32 TF0002-2A 0.39 19 4.8 TF0002-2B 0.34 14.57 3.47 TF0002-3A 0.36 16.23 3.9 TF0002-3B 0.34 14.71 3.51 TF0002-4B 0.36 15.86 3.85 T89-1 0.33 14.2 3.37 T89-18 0.35 14.1 3.23 T89-20 0.35 14.57 3.37 T89-24 0.36 14.39 3.34 T89-27 0.37 16.03 3.87 T89-3 0.36 14.89 3.5 T89-30 0.37 14.11 3.3 T89-31 0.38 15.44 3.63 T89-32 0.37 15.34 3.63 T89-34 0.37 15.56 3.71 T89-36 0.36 14.43 3.39 T89-4 0.37 16.4 3.93 T89-6 0.37 14.68 3.46 T89-7 0.33 14.25 3.36 T89-9 0.38 15.94 3.8

[0524] 1C

[0525] Results from the fibre measurements are presented in table 6.3 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00229 TABLE 6.3 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TF0002 1.00 1.09 1.03 1.16 1.03 1.03

Construction Group TF0052

[0526] This construct induces changes in fibre parameters. The average fibre width is 13% higher than of corresponding wildtype group. The maximum fibre width is 22% higher than corresponding maximum wildtype. The TF0052 construction group meets the fibre parameters selection criterion (2) and (4).

[0527] Table 6.4 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00230 TABLE 6.4 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TF0052-1A 0.3 12.77 3.11 TF0052-1B 0.33 14.4 3.46 TF0052-2A 0.34 15.9 3.86 TF0052-2B 0.36 18.22 4.49 TF0052-3A 0.34 18.54 4.71 TF0052-3B 0.34 14.52 3.49 TF0052-4A 0.35 15.94 3.9 TF0052-4B 0.32 15.33 3.76 T89-1 0.35 15.15 3.59 T89-12 0.34 14.79 3.44 T89-14 0.33 12.52 2.83 T89-16 0.34 13.67 3.14 T89-18 0.34 14.25 3.28 T89-19 0.33 13.82 3.17 T89-20 0.36 15.11 3.58 T89-21 0.34 13.42 3.14 T89-24 0.33 14.54 3.39 T89-25 0.37 15.2 3.58 T89-26 0.35 14.12 3.31 T89-27 0.33 13.52 3.12 T89-28 0.33 13.41 3.13 T89-3 0.3 13.3 3.18 T89-30 0.32 12.52 2.91

[0528] Results from the fibre measurements are presented in table 6.5 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00231 TABLE 6.5 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TF0052 0.99 1.13 0.97 1.22 1.00 1.02

Construction Group TF0058

[0529] This construct induces changes in fibre parameters. The average fibre width is 16% higher than of corresponding wildtype group. The maximum fibre width is 23% higher than corresponding maximum wildtype. The TF0058 construction group meets the fibre parameters selection criterion (2) and (4).

[0530] Table 6.6 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00232 TABLE 6.6 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TF0058-1A 0.34 15.29 3.74 TF0058-1B 0.31 15.07 3.66 TF0058-2A 0.36 16.98 4.21 TF0058-2B 0.34 15.89 3.89 TF0058-3A 0.36 15.28 3.67 TF0058-3B 0.36 15.62 3.77 TF0058-4A 0.37 16.97 4.14 TF0058-4B 0.39 18.7 4.57 T89-1 0.35 15.15 3.59 T89-12 0.34 14.79 3.44 T89-14 0.33 12.52 2.83 T89-16 0.34 13.67 3.14 T89-18 0.34 14.25 3.28 T89-19 0.33 13.82 3.17 T89-20 0.36 15.11 3.58 T89-21 0.34 13.42 3.14 T89-24 0.33 14.54 3.39 T89-25 0.37 15.2 3.58 T89-26 0.35 14.12 3.31 T89-27 0.33 13.52 3.12 T89-28 0.33 13.41 3.13 T89-3 0.3 13.3 3.18 T89-30 0.32 12.52 2.91

[0531] Results from the fibre measurements are presented in table 6.7 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00233 TABLE 6.7 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TF0058 1.05 1.16 1.05 1.23 1.03 1.20

Construction Group TF0097

[0532] This construct induces changes in fibre parameters. The maximum fibre width is 13% higher than corresponding maximum wildtype. The TF0097 construction group meets the fibre parameters selection criterion (4).

[0533] Table 6.8 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00234 TABLE 6.8 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TF0097-1A 0.38 16.6 4.07 TF0097-1B 0.36 14.84 3.53 TF0097-2A 0.33 12.65 2.92 TF0097-2B 0.37 15.92 3.8 TF0097-3A-1 0.38 17.11 4.13 TF0097-3A-2 0.33 14.05 3.37 TF0097-4A-1 0.34 12.27 2.79 TF0097-4A-2 0.33 13.03 2.98 TF0097-4B 0.3 12.34 2.9 T89-02 0.3 11.82 2.7 T89-07 0.36 14.1 3.25 T89-11 0.35 13.66 3.2 T89-17 0.31 13.26 3.15 T89-19 0.32 15.14 3.67 T89-26 0.32 12.06 2.7 T89-29 0.36 15.19 3.63 T89-31 0.36 14.51 3.44 T89-32 0.35 13.73 3.17 T89-34 0.33 12.93 2.96 T89-35 0.32 13.28 3.12 T89-38 0.34 14.69 3.52 T89-44 0.32 13.64 3.24 T89-46 0.34 13.96 3.26 T89-61 0.34 14.09 3.35 T89-68 0.29 12.49 2.99

[0534] Results from the fibre measurements are presented in table 6.9 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00235 TABLE 6.9 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TF0097 1.04 1.05 1.06 1.13 1.03 1.04

Construction Group TF0109

[0535] This construct induces changes in fibre parameters. The average fibre length is 11% higher than of corresponding wildtype group. The maximum fibre length is 25% higher than of corresponding maximum wildtype. The maximum fibre width is 23% higher than corresponding maximum wildtype. The TF0109 construction group meets the fibre parameters selection criterion (1) (3) and (4).

[0536] Table 6.10 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00236 TABLE 6.10 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TF0109-1B-1 0.35 15.06 3.54 TF0109-1B-2 0.35 15.83 3.8 TF0109-2A 0.34 15.4 3.69 TF0109-2B 0.35 15.7 3.75 TF0109-3B 0.36 15.73 3.75 TF0109-4A 0.45 21.49 5.12 TF0109-4B 0.37 16.82 4.08 T89-16 0.35 15.93 3.82 T89-17 0.34 15.56 3.79 T89-19 0.34 15.87 3.9 T89-2 0.33 13.88 3.25 T89-20 0.33 14.43 3.42 T89-24 0.34 14.52 3.46 T89-25 0.35 15.48 3.73 T89-26 0.32 14.93 3.61 T89-34 0.33 14.68 3.54 T89-35 0.36 15.84 3.77 T89-36 0.31 16.92 4.23 T89-37 0.31 15.95 4.02 T89-38 0.3 13.64 3.23 T89-4 0.33 17.48 4.44 T89-6 0.3 14.5 3.55

[0537] Results from the fibre measurements are presented in table 6.11 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00237 TABLE 6.11 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TF0109 1.11 1.08 1.25 1.23 1.13 1.10

Construction Group TF0116

[0538] This construct induces changes in fibre parameters. The average fibre width is 11% higher than of corresponding wildtype group. The maximum fibre width is 20% higher than corresponding maximum wildtype. The TF0116 construction group meets the fibre parameters selection criterion (2) and (4).

[0539] Table 6.12 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00238 TABLE 6.12 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TF0116-2A 0.33 14.84 3.55 TF0116-2B-1 0.34 18.24 4.52 TF0116-2B-2 0.34 15.66 3.7 TF0116-4A 0.37 15.85 3.76 TF0116-5B 0.33 12.15 2.82 TF0116-6A 0.33 15.2 3.69 TF0116-6B 0.37 16.5 3.94 T89-1 0.35 15.15 3.59 T89-12 0.34 14.79 3.44 T89-14 0.33 12.52 2.83 T89-16 0.34 13.67 3.14 T89-18 0.34 14.25 3.28 T89-19 0.33 13.82 3.17 T89-20 0.36 15.11 3.58 T89-21 0.34 13.42 3.14 T89-24 0.33 14.54 3.39 T89-25 0.37 15.2 3.58 T89-26 0.35 14.12 3.31 T89-27 0.33 13.52 3.12 T89-28 0.33 13.41 3.13 T89-3 0.3 13.3 3.18 T89-30 0.32 12.52 2.91

[0540] Results from the fibre measurements are presented in table 6.13 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00239 TABLE 6.13 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TF0116 1.02 1.11 1.00 1.20 1.10 0.97

Construction Group TFSTT001

[0541] This construct induces changes in fibre parameters. The average fibre width is 14% lower than of corresponding wildtype group. The minimum fibre length is 17% lower than corresponding minimum wildtype. The minimum fibre width is 30% lower than corresponding minimum wildtype. The TFSTT001 construction group meets the fibre parameters selection criterion (7) and (8).

[0542] Table 6.14 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00240 TABLE 6.14 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TFSTT001-1BX-2 0.31 11.68 2.7 TFSTT001-1BY-1 0.3 12.11 2.83 TFSTT001-1BY-2 0.25 9.57 2.51 TFSTT001-2A-1 0.28 12.87 3.06 TFSTT001-2A-2 0.36 15.23 3.65 TFSTT001-3B-1 0.35 15.26 3.63 TFSTT001-3B-2 0.35 15.66 3.77 T89-16 0.35 15.93 3.82 T89-17 0.34 15.56 3.79 T89-19 0.34 15.87 3.9 T89-2 0.33 13.88 3.25 T89-20 0.33 14.43 3.42 T89-24 0.34 14.52 3.46 T89-25 0.35 15.48 3.73 T89-26 0.32 14.93 3.61 T89-34 0.33 14.68 3.54 T89-35 0.36 15.84 3.77 T89-36 0.31 16.92 4.23 T89-37 0.31 15.95 4.02 T89-38 0.3 13.64 3.23 T89-4 0.33 17.48 4.44 T89-6 0.3 14.5 3.55

[0543] Results from the fibre measurements are presented in table 6.15 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00241 TABLE 6.15 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TFSTT001 0.95 0.86 1.00 0.90 0.83 0.70

Construction Group TFSTT004

[0544] This construct induces changes in fibre parameters. The average fibre width is 15% higher than of corresponding wildtype group. The maximum fibre width is 29% higher than corresponding maximum wildtype. The TFSTT004 construction group meets the fibre parameters selection criterion (2) and (4).

[0545] Table 6.16 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00242 TABLE 6.16 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TFSTT004-1A 0.31 14.81 3.63 TFSTT004-2A-1 0.35 16.07 3.84 TFSTT004-2A-2 0.37 19.54 4.92 TFSTT004-2B-1 0.37 16 3.86 TFSTT004-2B-2 0.35 14.67 3.53 TFSTT004-3B 0.3 14.73 4.13 TFSTT004-4B-1 0.35 16.72 4.1 TFSTT004-4B-2 0.37 16.21 3.88 T89-1 0.35 15.15 3.59 T89-12 0.34 14.79 3.44 T89-14 0.33 12.52 2.83 T89-16 0.34 13.67 3.14 T89-18 0.34 14.25 3.28 T89-19 0.33 13.82 3.17 T89-20 0.36 15.11 3.58 T89-21 0.34 13.42 3.14 T89-24 0.33 14.54 3.39 T89-25 0.37 15.2 3.58 T89-26 0.35 14.12 3.31 T89-27 0.33 13.52 3.12 T89-28 0.33 13.41 3.13 T89-3 0.3 13.3 3.18 T89-30 0.32 12.52 2.91

[0546] Results from the fibre measurements are presented in table 6.17 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00243 TABLE 6.17 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TFSTT004 1.03 1.15 1.00 1.29 1.00 1.17

Construction Group TFSTT017

[0547] This construct induces changes in fibre parameters. The minimum fibre length is 17% lower than corresponding minimum wildtype. The TFSTT017 construction group meets the fibre parameters selection criterion (7).

[0548] Table 6.18 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00244 TABLE 6.18 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TFSTT017-1A-1 0.38 14.46 3.57 TFSTT017-1A-2 0.25 12.95 3.23 TFSTT017-1B 0.28 12.88 3.41 TFSTT017-2A-1 0.35 16.02 3.89 TFSTT017-2A-2 0.33 13.46 3.1 TFSTT017-2B 0.31 13.48 3.25 TFSTT017-3A 0.32 14.97 3.63 TFSTT017-3B 0.3 12.75 3.01 TFSTT017-4B 0.29 11.93 2.77 T89-1 0.35 15.15 3.59 T89-12 0.34 14.79 3.44 T89-14 0.33 12.52 2.83 T89-16 0.34 13.67 3.14 T89-18 0.34 14.25 3.28 T89-19 0.33 13.82 3.17 T89-20 0.36 15.11 3.58 T89-21 0.34 13.42 3.14 T89-24 0.33 14.54 3.39 T89-25 0.37 15.2 3.58 T89-26 0.35 14.12 3.31 T89-27 0.33 13.52 3.12 T89-28 0.33 13.41 3.13 T89-3 0.3 13.3 3.18 T89-30 0.32 12.52 2.91

[0549] Results from the fibre measurements are presented in table 6.19 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00245 TABLE 6.19 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TFSTT017 0.93 0.98 1.03 1.05 0.83 0.95

Construction Group TFSTT038

[0550] This construct induces changes in fibre parameters. The average fibre width is 16% higher than of corresponding wildtype group. The maximum fibre width is 21% higher than corresponding maximum wildtype. The TFSTT038 construction group meets the fibre parameters selection criterion (2) and (4).

[0551] Table 6.20 contains fibre measurements data for specified construction group and corresponding wild type group.

TABLE-US-00246 TABLE 6.20 Construction Fibre Fibre group Length Width CWT Individual (mm) (μm) (μm) TFSTT038-1A-1 0.35 16.43 4 TFSTT038-1A-2 0.35 16.88 4.1 TFSTT038-1B 0.36 17.48 4.27 TFSTT038-2A 0.37 18.37 4.55 TFSTT038-2B 0.34 16.41 4.04 TFSTT038-3A 0.32 15.95 3.93 TFSTT038-3B 0.36 14.95 3.56 TFSTT038-4B 0.32 12.89 2.98 T89-1 0.35 15.15 3.59 T89-12 0.34 14.79 3.44 T89-14 0.33 12.52 2.83 T89-18 0.34 14.25 3.28 T89-19 0.33 13.82 3.17 T89-20 0.36 15.11 3.58 T89-21 0.34 13.42 3.14 T89-24 0.33 14.54 3.39 T89-25 0.37 15.2 3.58 T89-26 0.35 14.12 3.31 T89-27 0.33 13.52 3.12 T89-28 0.33 13.41 3.13 T89-3 0.3 13.3 3.18 T89-30 0.32 12.52 2.91

[0552] Results from the fibre measurements are presented in table 6.21 as ratios of average fibre length (AFL), average fibre width (AFW), maximum fibre length (maxFL), maximum fibre width (maxFW), minimum fibre length (minFL), minimum fibre width (minFW) of specified construction group relative to corresponding wildtype group.

TABLE-US-00247 TABLE 6.21 Average Average Max Max Min Min Construction Fibre Fibre Fibre Fibre Fibre Fibre Group Length Width Length Width Length Width TFSTT038 1.03 1.16 1.00 1.21 1.07 1.03

Example 7

Selected Constructs Used for Tobacco Transformation

Tobacco Transformation

[0553] Based on growth data from poplar experiments, a selected set of constructs, namely CaMV 35S: over-expression DNA constructs TF0097, TF0132 and TFSTT019, were used for transformation into tobacco (Nicotiana tabacum cv. SR1). Plants were transformed and regenerated essentially as described in Nilsson et al. (1992), but using leaf disc explants.

[0554] Approximately 10-15 independent lines were generated for each construct. One such group of transgenic plants produced using one construct is hereafter called a "construction group", e.g. different transgenic plants emanating from one construct. Each transgenic line within each construction group, e.g. TF0555-01, TF0555-02, TF0555-03 and so on, are different transformation events and therefore most probably have the recombinant DNA inserted into different locations in the plant genome. This makes the different lines within one construction group partly different. For example it is known that different transformation events will produce plants with different levels of gene over-expression.

Plant Growth

[0555] The transgenic tobacco plants, comprising three construction groups each having 9-15 independent lines, were grown together with 14 wildtype control plants, in a greenhouse under a photoperiod of 18 h and a temperature of 22° C./18° C. (day/night). The plants were fertilized with Weibulls Rika S NPK 7-1-5 diluted 1 to 100 (final concentrations NO₃, 55 g/l; NH₄, 29 g/l; P, 12 g/l; K, 56 g/l; Mg 7.2 g/l; S, 7.2 g/l; B, 0.18 g/l; Cu, 0.02 g/l; Fe, 0.84 g/l; Mn, 0.42 g/l; Mo, 0.03 g/l; Zn, 0.13 g/L). Plant height and diameter was measured regularly during growth in the greenhouse.

[0556] Observed growth effects in tobacco transformants included faster regeneration of tobacco plants transformed with construct TF0132, where regenerated plantlets had markedly larger leaves during early tissue culture phases. Also, in tobacco plants transformed with either of the selected constructs (i.e. TF0097, TF0132 or TFSTT019) a longer period of vegetative growth and hence later flowering than wild-type SR1 plants was observed.

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Sequence CWU 1

1

20311044DNAPopulus trichocarpa 1atggctgtgg agcttatgat ggggtattct ggtgatagtt ttgctacaaa aatgcaagag 60aatgatgtga gagaagccgc aactgctggg atacaaagcg ttgaggaagt cataaaactg 120ctcaaacaaa atcaactgga acagcaacaa aaacaacaat actaccaaga gttgtctgca 180gcctcttcaa gttccaatct tggcacggat aatatcatgg ctgttactga tatggccgtg 240aacaatttca aaaaggttat ttctttactg ggtcgcacca caagaactgg ccatgctcga 300tttagaagag ctcctgttgc ctgccctcct caacaacaaa tacaagaacc agaaccagga 360ccgcaacagc aaaaacagca accaccagag caaggctctg cttttagagt ttatcaaccg 420accccaattc atcgtctccc tcctttgcct cacaatcagc aacaaaagac actgctggtt 480acgaaaaatg gattatcaga tcggaatgaa atggctacta cgatcaattt cgctaattcg 540ccaacaatat ctgctccttc tttcttgtct tctttaacag gggaaactga tagcttccag 600tgttctaagt cttctgggtt tcagtttacc caaccttctg ctggtaaacc tcctttgtcc 660tcttcttctc ttaagagaaa gtgcaactcc atggatgatg ctgctctcaa gtgtggctct 720tcttctagtc gctgccactg ctccaagaaa agcaggaaat caagaattaa aagggtggtt 780agagttcctg caattagtag taagatggct gatatcccac ctgatgatta ttcctggaga 840aagtatggcc aaaagcccat caaaggctct cctcatccca ggggatacta caagtgcagt 900agcgtgagag gatgtccggc acgcaaacac gtggagagag ctctagatga ctcgatgatg 960cttattgtga cctatgaagg ggaacacaac cactctcatc caatcgatga tgcacctggt 1020gctcttgtcc ttgaatcatc ttaa 10442474DNAPopulus trichocarpa 2atgtctttga acatgatcaa ctcttatgct tctaccgact ttgaaggtac taggggaaat 60ggattgttgg gattgatgca agagatgcaa gctccagcca gtttaaattt tgatgacatc 120tctcagaaca gaggctttgt tgggtctgga attgagggga aattgggaaa gaataaggga 180gaaaagaaga taagaaagcc caagtacgct tttcaaacaa ggagccgagt tgatatcctt 240gatgatggat atcgatggag aaagtatggt caaaaggccg tcaagaacaa caaatttccc 300agaagctact atcggtgtac acatcaaggg tgcaacgtga agaagcaggt tcagagatta 360acaaaagacg aaggagtcgt ggtgacaacc tatgaaggaa tgcattctca tcagatcgaa 420aagtctccag ataacttcga gcatatcttg agccagatgc aaatctactc ctga 47431428DNAPopulus trichocarpa 3atggagatca aggaatcaga gagggtggtt atagctaaac cagtagcttc caggcctagt 60tactcaaatt ttacgtcttt ctcagagttg cttgcgggtg ccatcaatac cacaccctct 120aatgtttgtt ctggaacagc agttgctgcc attagaccaa aaacagtgag gttcaggcca 180ctggtgaatc gtgctccagg tgcactggtt tcttcacagg ctggactctc tggaacagca 240gttagcaatt cgtttaataa agcttcaagt actgatagca aagccactat catatataaa 300ccacaggcaa agcttgtgtc aaaggcaact gtttctctac tggcgaatat gggaaacttc 360aatactaatt cacaacaaat gttacaacca gttgagactc gtcctcagct ttcaaaacaa 420gataaacaca atttctcatc ccagcttacc tcaaatcctc atcagaatat tccatcccct 480gcagaagcag accacacaac tgaaccctta aggttaacat ctctgaacca ggaggaggat 540cctaaaactt tatctcatgc atctaatggg gaccgacctt catatgatgg ttataattgg 600aggaaatatg gacaaaagca agtaaaagga agtgaatatc cacggagcta ctacaagtgc 660acctatccaa actgtccagt gaaaaagaag gtcgaaagat catttgacgg gcagattgca 720gaaattgtct acaaagggga acacaaccat tcaaagcctc agcctccaaa gcgcaactca 780tcaggaacac aaggactatc tgatggcaat gctccagata ggaacagcat cccattatgg 840agtaatcaac tcaatgaaag aaatgaaggc tctgaaggta gagaagaaaa tcagaatgaa 900attggattac cagtgcattc aatttatcag ggtaaagctc caccatctta tgatcctgct 960ggaaccggca caattaatgc tggtacggga acttctgata actcatgtgg tgtcagcggg 1020gaatgtgatg atggaagcaa gggattggag ggagccaatg atgaacctaa aagtaaaaga 1080aggaaaactg agatacaatc cactgaaggt ggcatgtcag gggagggagt acaagagccc 1140cgtgtagtgg tgcaaagttc cacagattct gagattttgg gggatggttt tcgatggaga 1200aaatatgggc agaagattgt gaaggggaat ccatatccca ggagttacta tagatgtacc 1260agtatcaaat gcaatgttcg caagcatgtg gaaagagtat cagatgatcc aagagctttt 1320attacaacat atgaaggaaa acataaccat gagatccctc tgaagagcac aaacctagca 1380gcctttgaac ctgattcaca ggcacctact actagagtca agcagtga 142841068DNAPopulus trichocarpa 4atggaggagg ttgaggaggt acacaaggca gctattgaga gctgcaatag agttattggc 60cttttgtgtc aacaaaaaga tcaagtccag ggtaggaact taatggtgga aactagagag 120actgtgttta agtttaagag agttatatct cttttaagca ctggtttagg tcatggaaga 180gtaagaaaga tgaagaagct tagaccatct ttgccccaaa acatcttcct agatagtcct 240aattgcaaaa cagttttatc accaaaacct ctccaaatgg tgcctcctaa ttttcttgaa 300accccactta ctgacatgga tgctaagtct aaaccttcta tccaaatttc ccagaaaatg 360cttcttgaaa acccagtact tgagttgaac tcaaatatca ggccccctgt gcaaattatg 420caaacaaaac caccacaaaa cttccagctt ctccaacagc atcagcagac acagaggatg 480cactttcagc agcagcagca gcagcaaatg aagtatcagg ctgatagggt gtattctagg 540agtaatggtg ggataaacct taagtttgat gggtctactt gcacaccaac catgtcatcc 600acaagatcat tcatatcatc tctgagcatg gatggtgctg tgtctacttt tgatggagac 660tctttccatt tgattggtat gcctcactca tctgatcaca tctcacaaca aacaaggagg 720aggtgttctg gtagaggaga agatgggaat gccaaatgtt ctagcagtgg taaatgccat 780tgttcaaaga ggaggaaact gagggtgaag agatctatca aagttcctgc aattagcaac 840aaggtggcag atattcctcc tgatgagtat tcatggagga agtatggaca aaagccaatt 900aagggctctc cgcatcctag gggatactat aaatgtagca gtctgagggg ttgcccggca 960aggaagcacg ttgagagatg cctggaagac ccttcaatgc taattgtaac ctatgaaggt 1020gagcataacc attccaggtt gatctcatca cagtctgcgc atacatga 106852157DNAPopulus trichocarpa 5atggctggga ttgatgataa cgttgctata attggtgatt gggtacctcc tagtccaagt 60ccaagagctt ttttctcagc aatgttaggt gatgatatta actcaagaac aatcccagaa 120tctcctgggg agaaaagaaa tgaagggctc tatctgggac aaccagagca gatggcaaca 180ggaaattctg agaaaaagga tggagcacaa actagtggtg ttcagttgac tgaattaggt 240acattttctg agcaaaaatc aaactttcgt ggaggtcttg ttgaaagaat tgcagccaga 300gctggattta atgcaccgcg gttgaataca gaaagtatca ggtccactga aagttctatg 360aaccctgaga ttaggtcgcc ttatttgaca ataccacctg gtctcagccc gacaaccttg 420ctggaatctc cagttttcct ttcaaatttg gcacagccat ctccaacaac tggaaaattt 480tctttttttc caaatggtaa caccaagaac tccacagtgg gctctgaccc tcctgataaa 540agtaaagaaa ctttctttga caacattgat tcatcttcat ttgcgttcaa gcctatggga 600gaatcaggtt cttttttcct tggtggaaca agcaagcaat cctttcccag tatcgatgtt 660tctgttcact ctgagaatgc ttttcagtcg catggtgtgg aacccgccaa aactcagttt 720gagagtagaa acagcctcca tttccgagca gaattctcca aattgactac tgaaaaggat 780aatggatgta ttactgtagc agatcagagg acctttgaca ctgttgctgg caacgccgag 840cattcttccc ctcttgctga gcaacaagat gaagaaggag atcaaagagc cagtgaagat 900tcaatggctg ctggaggcac accatctgag gatgcatata attggagaaa atatgggcag 960aaacaagtaa aaggcagtga gtatccacgg agttattaca agtgcacgca tccaaattgc 1020ccagttaaga agaaagtgga acgttctcat gagggccata taacagagat catatataag 1080ggggcacaca accatcccaa gccaccaccc aaccggaggt cagccactgg atcaatggat 1140acacaactgg atattcctga acaagctggg ccacaggttg gtgctgttaa tgattcggtt 1200tgggcaggta cacaaaaagg aactgctgca ggaactcctg attggaggaa tgacaatgtc 1260gaggtttcat cttcagcatc tgggggccta ggccctgaat tcgggaatcc atcttcctcg 1320gtacagggtc agagcggaac tccctttgag tcagctgatg ctgtggatgc ctcgtctacc 1380ttttcaaatg atgaagatga tgatcgggct acacatggca gtgtaggtta tgatggtgaa 1440ggagaagaat ctgagtccaa gagaaggaaa gttgaaacat atgcaaccga aatgagtgga 1500gccaccagag caatccgtga gcctagagtt gtggtccaga caaccagtga ggtggatatc 1560cttgacgatg gatatcgctg gagaaagtat ggacagaaag ttgttaaagg aaatccaaat 1620cctaggagtt attacaagtg cactagcgca ggctgcacag tgagaaagca tgtggagagg 1680gcatcgcatg accttaagtc agtgatcact acgtatgagg ggaagcacaa tcatgatgtc 1740cctgctgctc gcaatagcaa ccatgtcaac tctggcacct ctaatgccac accagcccaa 1800gctggcatcg cagttcaaac ccaagttcac agacctgagg catcccaagt tcacaacagc 1860atgactagta gatttgagag gcctcctgca tttggatcct tcaggcagca gctggggcct 1920tcggctggct tctcatttgg aatgaatcaa cctgggtttg ccaatatggg aatggctggg 1980ttaggtccag ggcaaccaag gatgccagtt atgcccatgc atccatactt agcacagcaa 2040cgtgcagtga atgaaatggg gttgatgatg ccgaaaggag aagcaaaggt ggagccagtg 2100acctccaaca atccaacagt gtatcagcaa ataatgagta ggctgcctca gatgtaa 21576906DNAPopulus trichocarpa 6atggctgtgg atctagttag gtattcaaag atggaagatc agatggctat acaagaagct 60gcatcagctg ggctcgagag catggagcac ttgatctttg cattctctaa ccaaactcga 120caaagccacc aacttgactg cggagaaatc acaaacttca ccgttgctaa gttcaagcaa 180gtcatctcca tgttgaaccg gaccggtcat gcccgttttc gccgtggacc aacttcttct 240ccttcttcct acccggttcc cgtccgacct gtccctcaag agcctcaaaa actgaacctt 300gattttgtta acagtaatag cccccctaaa gctgagtcga aaaatgacct gtctttgggt 360agtcagtatt caaaggatag ccttagctct ggcaccacta cctcatcctt cgtgtcttct 420gttacagctg atgggagtgt ctctaatggg aaacaaggtg gctcttctct tttcggaact 480caagcgcgat ctaccggaaa accacctctc tcatcgaccc accgcaagaa atgccacgat 540catgccctct ccgccagaaa gatctcctcc ggtggaagct gtcattgctc caaaagaagg 600aaatcaaggg ttaagaggac aataagggta ccagccgtga gttccaagat tgccgatata 660ccagcagatg agtactcatg gagaaaatat ggtcaaaagc caatcaaggg ctcaccatac 720ccaagagggt attacaagtg tagcagtgtt agaggatgtc cagcaaggaa gcatgtggag 780cgtgccgtag atgactcggc catgcttatc gtgacttacg agggggagca ccgtcactca 840catactccgt tgccgggaga cgtcaccgcg agtgctgcaa tgcgacacgt gtttcactca 900acatga 90671044DNAPopulus trichocarpa 7atggaggggg ttgaagaagc taaccgggca gctgtggaga gctgccatag agttataagt 60ttgctatccc aaccccaaga tcaggttcaa tataggaatt taatggtgga aactggagag 120gctgtgttta ggttcaagaa agtagtttcc cttttaaata ctggtttagg tcatgcaaga 180gttcgaaaac ttaagaagtt accgacccct ttatcccaaa gcatcctctt agacaaccca 240ctgagcagta cagaccaccc atccaaaaca ccccagtttc tccagtccag tagttacctg 300gaaagccaac caattcaaga attgggctca attgctaaaa attgtctatc tttgggaacc 360ccatccctgg aattgagttc aaatgggaaa aaccctcttc agcttggcca acccacgcca 420gcagcgcact atcagttcct tcagcaacag caactgcata ggctacagct tcaacagcag 480cagcaaatga agcagcaagc tgagatgatg tttagaaaaa gcaatagtgg gattagcttg 540aatttcgata gttctagctg cactcctaca atgtcatcta ccagatcttt tatatcgtcc 600ttgagtattg atggtaatgt ggctaatttg gaaggaagtg cattccattt aacgggggcg 660gctcgctcct cagatcagag ttcacagcaa cacaagagga aatgttctgg aaggggagaa 720gatggaagta tgaaatgtgg aagcagcgtt agatgtcatt gctcaaagaa gaggaaacat 780agggtgaaga ggtcgatcaa ggttcctgct attagcaaca agcttgctga tatccctcct 840gatgattact catggagaaa gtatgggcaa aagccaatca agggttctcc tcatcccagg 900ggatattaca aatgtagcag tatgagaggt tgtcctgcaa ggaagcatgt ggagaggtgc 960ttggaagatc cgtccatgct tattgttacc tatgaaggtg aacataacca cccaaggatt 1020ccagcacaat ccacaaacac gtaa 104481098DNAPopulus trichocarpa 8atggaattgt ccatggaatg ggagcacaag actctaataa gtgagctaac tcaagggaaa 60gagctagcga aacagcttag caaccatctt aacccttcct catctctcga agcacgtcaa 120ttcctagtgg ataagatact ttcttcttat gagaaagcac tttcaatgct aaattggggg 180gctttggctg ctgatcaacc aaagcccaca attggcacag tcgaaccact gcattcattt 240gccaacagta gtcctaggag tgaggtctct gatcaggatt gcaaggagga atgtaacaaa 300gatgtttaca agaagagaaa aatacaacct cgatggactg agcaagtgaa ggattgttca 360gggaccggtc tagaagggcc tcttgatgat ggttatagct ggaggaaata cgggcaaaag 420gatattctcg gagctaattt tccaagagga tactacagat gcactcaccg ccattcccaa 480ggctgtttgg ccacgaagca agtgcaaaga tcagatgagg accattcaat ttttgaagtg 540acctatcgag ggagacatac atgtaaccaa gcctctcctt cacccgtggc atcgccttcc 600ccaaaaaatg attgctcaaa acaaagcaag tatcatcgta agcagcagca gcagcgtcaa 660gagaaaccaa agccaacaga ggagatattt gttaactttg gatcagacat tgttcaagtt 720aaaaatgagg acttgggctc caaggacgac attttcccat ccttttcctt cccatgtaca 780tcattcggga atgaaaacga ggaaaacaac attttcaccg agtccatgat ggaaaacaac 840ttcttgggta gcttttctcc aacatttata tctccggcaa catctgaatc caactacttc 900tcaatgtcac catgccatat gaacagcttt ggaataggtt accaaaatgt gcagacccca 960gagtctgaac tcaccacgga gataatatca gccccaactt cggtaaccaa ttcaccaatc 1020ggggattttg atatctcaat cgataatgta gattttgata ccaccttccc atttgacaac 1080ccagatttct ttgcttaa 109891074DNAPopulus tricocharpa 9atgatggaag aactttacgg tcttcaatcc accagctctg attactcttt gcaggttcca 60tcagagaaca tggtttctcc agtggctaat tactaccatc ctgtggggtt cacctcccca 120gctggggagc catcatttcc tgtttttgga tcggagcaga tgttttgtgg ttcctcagta 180tctgatgctg cttcaatggg ggccgagttg catcagaagc agcagcagca gagtgggggt 240gttggtgttg gtgatcatag taatagaaat aattcagagg aggtttcttg tgcaattagg 300gctaaaattg cttctcaccc gctttaccct aagttacttg aagcttatat tgattgccag 360aaggtgggag caccgccgga gatggcttat ttattagatg aaatccggct agtgaatgat 420gtttccaagg ggtccaatga tactgtcgcc tcttgcttgg gtgccgatcc cgagctcgac 480gagttcatgg aaacttactg tgacgttctg atgaagtata aagcggatct ctcaaggcct 540ttcgatgaag ccactacttt cttgaatgat atagaagctc agtttaatac tctctgcaac 600ggtccttcca gaagccaagt ttatggtctc cctctctctc tctcactctc tattttacta 660ctcagtatgt tatctaatga agctgctggt tcatctgatg aagatgccag tggaggagag 720gcaggcatgc aagattcaac ccgtataaac gaggatcgag agctgaagga caaactatta 780cgtaaatata gtggttacat aagtacccta aagcatgcgt tttcaaaaca gaagaagaaa 840ggaaaactac caaaggaagc aaggcaaatc ctgttaaact ggtggaacat tcataacaaa 900tggccatacc caacggaagc tgataaggtt gctttggccg aatcaactgg cctagatcag 960aaacagataa acaactggtt catcaatcaa aggaaacgtc actggaaacc atcagagaac 1020atgcaatttg ctgttgtgga tagtctgtat ggtccctttt tcatgaacga ctga 1074101707DNAPopulus tricocharpa 10atggaaagag ataagttgtt tgtgagcgag ggagccaaca cagcagcaac catttggaat 60tcttgcagtt ttggaatgga aatgcaagcc aatgagctga gttgcggtcc agagaaactt 120gccaattgct ttctcaatcc caattgggac aactcattgg atcagagcga tccctttgag 180tctgctttga gctccattgt ctcatcacct gttgcatccg gtgccaacgc aaacgccaac 240gccattccta atgctggcgt tggtggtgac agtcttatga ttagagaact tattggaaga 300ctaggaaaca tttgcaattc tggagacatt tcactacaat cttttgttaa caataataac 360aatagcacta acacttcttg ctatagtacc ccaatgaatt cccctccaaa gctgaatctc 420tcgatgatgg attcacaaat gagaggaaac ctgccaattc ctggaaacag cgtagtaaag 480catccaggtt tagcaccatt tccagctgat tttgtagaga gggctgcacg atattcttgc 540tttggtagca acaatcctgg aggcatcaat aaacaattcg gattgaatga atccgaattg 600attaataggt tgatgccacg agtagaacct ggtaagctct cgagagtttc gagtaacaat 660tcaatgaagg tcactgtatc gcaagcaaat gttcaagaaa gcaacaagag ctcaccccag 720gatgggagtt tgaattctga aaagaaattc agtaggcagt caaggcctac aacatcagag 780aatggagatt ccagggaaga atcttcattg tctgagcagg tcccaggtgg gaaattgagc 840atgaaatccc agaatgacgc caattccagg aaaagaaaat caattcccag aggaaaagcc 900aaagaaactc cctcttcatc tccatctgct tctgatgtca aggttgcagc agagaatgat 960gaatcgaagg caaaaagaag caaatcggat gaaactaatg gcagtgacaa ggatacagca 1020aaggaaaagg aagaagaaaa tggaaatcag aaacagaaca aaaataattc aaagccgcca 1080gagccaccaa aggattatat ccatgtcaga gccagaaggg gtcaggctac agatagccac 1140agtcttgctg aaagagttag aagagagaaa atcagtgaaa gaatgaagtt cctccaggat 1200cttgttcccg gatgcaataa ggttactggg aaagcagtga tgcttgacga gattataaac 1260tatgtacagt cattgcagcg ccaggttgag tttctgtcaa tgaagttgtc atctgtgaat 1320ccgaggatgg agatcaacat ggaaactttg ttgtccaagg atattttcca atcccgtgga 1380tctatgccac atagtcttta tccattagat gcctccacgc cggtattccc ttatggttac 1440caatcccagc aagggttagc cctgcaaaat ggcatgccaa gcaatgccga aacccagttc 1500tctatgaacc cattaaatgc tgcgttgcgg cgaaacccga gcatgcattt gccacacctt 1560gatggtttcg gtgatcctgc tgctcttcag gcctcagcca tgtgggaaga cgaccttcaa 1620agtgttgtgc agatgggata tggtcagaat catcaggaga gctttcaagg ctcagtgccc 1680tcaactcaca tgaaaattga gctataa 1707111176DNAPopulus tricocharpa 11atgtctgttg gtgtaaacac agaaacccta gtgagagttg aggagataca aggaagagga 60agagggcttg tatccactca gcccttaaga ggtggccaaa tcgtcctcat agattctcca 120atcctcctct attctgcact ccctttaacc aaacagcaac actcaacctt cctttactgt 180gacaaatgct tcaaaactat acaatcagca tcagtatctt gtcccacctg ctctcaccag 240cgtttctgta gccccacttg cctttccgct gctctggcat cttctcacac tccttgggtc 300tgccaatctc ttagccgcct ccgcgactgc caagattttc tacagcatca ttctgtggaa 360cgacaaatcc aagctcaatt cctcgttgct gcttacaatc ttgcattcgt ttcaccttcg 420gattttcaaa ttttgctgtc gctgcaaggc cgcgctgaag atgaagatcc agctattgtc 480cagtctctgc attctgtaat ctcatctctt tgtcctcctc caccaattga ggggttctct 540tttagcttgg aacttattgc ggctttggtt gcgaaagaca gatttaatgc tttcggatta 600atggagcctt tgaatcttaa tgaagagaat ggtgggcaaa gatcggtgag ggcttacggt 660atttatccta aggcatctct ttttaatcac gattgccttc caaatgcttg taggtttgat 720tatgttgata cgaataatag tgggaacact gatattgttg ttagaatgat tcatgatgtt 780ccgcaaggaa gggagatttg tttgagttat tttcctgtaa acagtaatta ctccactaga 840cggaaaagat tgcttgagga ctatggattt acctgtgact gtgatcgttg taaggtcgaa 900gctacttggt ctgatgacga gggtgatggt gatgataatg ataatgaggt gatggaggag 960gatgtcgatg agccaatgga ggccgaatct gatggtgaag aaatcggcaa tgataatagc 1020actgactttc ctcatgccta tttctttttg cgatacatgt gtaatcgaaa taattgctgg 1080ggtacactgg ctccttttcc tccttctgat gctaagccat caaatttatt ggagtgcaat 1140gcctgtggtg atatcaagaa tgatgaggtt tgttga 1176121008DNAPopulus tricocharpa 12atgtatcagc tggagagcgt tcctagttca agttcggtcc ataaaaactt attagttaat 60gatcagtatt tagattgtga tgatatgaca atggatccca tcaatggagg gaacaatctc 120aacaacaatc ctaatcttgc ctcaaagcaa agattgcgtt ggactcatga gcttcacgaa 180cgctttgttg atgctgtggc tcagcttggt gggccagatc gggctacgcc taaaggtgtt 240cttagagtca tgggtgtgca aggcttaaca atataccatg ttaaaagcca tttacagaaa 300tatcgacttg caaaatacct tcctgactcc tcatctgatg ggaaaaaagc ggacaagaag 360gaaacagggg atatgatttc caatttggat ggttcatctg gcatgcaaat tacagaagca 420ctcaagctgc agatggaggt gcagaagcgt ctacatgagc aattggaggt acagagacag 480ctacaattac gcatagaagc ccaagggaag tatttgaaga agataattga agagcaacaa 540cgattgagtg gagttcttga agatgtgcct ggctcggggg tcactgcccc agtatcaggt 600gataactgcc cagaatctga caagacagac ccagcaaccc ctgccccaac ttctgaatca 660cccctccaag acaaggctgc caaggaacgt gccccagcaa agagcctttc aattgatgaa 720tcattctctt ctcagcctga gccactgaca ccagattcac gttgcaatgc tggctcccca 780gcagagagcc ctagaggtga gagatctatg aagaagcaac gggtaagcat gggtgtaaca 840tatggtaaac aagagatggt tcttacacac cagatactcg agtcaagctt aaattcctat 900ccacgaccac actctgcctt cctgggtaga gagcagtttg atccttcatc tgggttatca 960atgggaatcg aagatcaaat ggagaaagtt tcaggcagtg atgtttag 1008131101DNAPopulus tricocharpa 13atgtctccac cactacttgg tgttgtggag gaggagggtc atagtaatgt cactctactg 60gcttctccgg cctcagcaga aagtgcatgc ctgaatggtt tggaattgaa agagcgtaac 120tacatgggtt tgtccgattg ttcttccgtg gacagctcgg cagtctctgc agcatctgat 180gaaagaaaga ctagtttaaa tctgaaggct

actgaattgc ggcttgggct tcctgggtcc 240cagtctccag aaagaaatca tgagctttcc ctgttgagct cggcattact tgatgagaag 300cccttcttcc ctttgcatcc ctcaaatgat ggtcactact cctcaacaca gaaaaatgtt 360gtttcgggta acaagagagt gttctctgat gccatggatg agttttcaga gagcaagttt 420ctgtcaaatt cggaggtaaa tgcaatgctc tcacccaggc cctcaccgaa catgggattg 480aaacctggca tgttggagaa ccttggagtt caacaagcta aagtgaaaga gatagtagcc 540ccaaaggcag gacaagagag acctcatgcg gcaaacgaga ccagaccact tcgtaacagc 600tccgcaaaca acagcagtgc acctgctcca aaggcacaag ttgtgggttg gccacccatc 660aaatcattta ggaagaattc ccttgccacc acctcaaaga acacagaaga agttgatggt 720aaagcagggc caggtgcttt atttatcaaa gtcagcatgg atggtgctcc ttatcttaga 780aaagtggatt tgagaaacta ctctgcatat caggaattgt cttctgccct cgagaagatg 840ttcagctgtt tcaccatagg ccaatatgga tcccatggag ctccaggaag ggagatgctg 900agcgagagca agctgaagga tctgctgcat ggctcagaat atgttctcac ttatgaggat 960aaagacggag actggatgct tgttggcgat gttccctggg agatgtttat tgaaacatgc 1020aagaggctga ggatcatgaa gagctctgat gccattggcc tagccccaag ggccatggag 1080aaatgcaaaa acaggaatta g 110114347PRTPopulus tricocharpa 14Met Ala Val Glu Leu Met Met Gly Tyr Ser Gly Asp Ser Phe Ala Thr 1 5 10 15 Lys Met Gln Glu Asn Asp Val Arg Glu Ala Ala Thr Ala Gly Ile Gln 20 25 30 Ser Val Glu Glu Val Ile Lys Leu Leu Lys Gln Asn Gln Leu Glu Gln 35 40 45 Gln Gln Lys Gln Gln Tyr Tyr Gln Glu Leu Ser Ala Ala Ser Ser Ser 50 55 60 Ser Asn Leu Gly Thr Asp Asn Ile Met Ala Val Thr Asp Met Ala Val 65 70 75 80 Asn Asn Phe Lys Lys Val Ile Ser Leu Leu Gly Arg Thr Thr Arg Thr 85 90 95 Gly His Ala Arg Phe Arg Arg Ala Pro Val Ala Cys Pro Pro Gln Gln 100 105 110 Gln Ile Gln Glu Pro Glu Pro Gly Pro Gln Gln Gln Lys Gln Gln Pro 115 120 125 Pro Glu Gln Gly Ser Ala Phe Arg Val Tyr Gln Pro Thr Pro Ile His 130 135 140 Arg Leu Pro Pro Leu Pro His Asn Gln Gln Gln Lys Thr Leu Leu Val 145 150 155 160 Thr Lys Asn Gly Leu Ser Asp Arg Asn Glu Met Ala Thr Thr Ile Asn 165 170 175 Phe Ala Asn Ser Pro Thr Ile Ser Ala Pro Ser Phe Leu Ser Ser Leu 180 185 190 Thr Gly Glu Thr Asp Ser Phe Gln Cys Ser Lys Ser Ser Gly Phe Gln 195 200 205 Phe Thr Gln Pro Ser Ala Gly Lys Pro Pro Leu Ser Ser Ser Ser Leu 210 215 220 Lys Arg Lys Cys Asn Ser Met Asp Asp Ala Ala Leu Lys Cys Gly Ser 225 230 235 240 Ser Ser Ser Arg Cys His Cys Ser Lys Lys Ser Arg Lys Ser Arg Ile 245 250 255 Lys Arg Val Val Arg Val Pro Ala Ile Ser Ser Lys Met Ala Asp Ile 260 265 270 Pro Pro Asp Asp Tyr Ser Trp Arg Lys Tyr Gly Gln Lys Pro Ile Lys 275 280 285 Gly Ser Pro His Pro Arg Gly Tyr Tyr Lys Cys Ser Ser Val Arg Gly 290 295 300 Cys Pro Ala Arg Lys His Val Glu Arg Ala Leu Asp Asp Ser Met Met 305 310 315 320 Leu Ile Val Thr Tyr Glu Gly Glu His Asn His Ser His Pro Ile Asp 325 330 335 Asp Ala Pro Gly Ala Leu Val Leu Glu Ser Ser 340 345 15157PRTPopulus tricocharpa 15Met Ser Leu Asn Met Ile Asn Ser Tyr Ala Ser Thr Asp Phe Glu Gly 1 5 10 15 Thr Arg Gly Asn Gly Leu Leu Gly Leu Met Gln Glu Met Gln Ala Pro 20 25 30 Ala Ser Leu Asn Phe Asp Asp Ile Ser Gln Asn Arg Gly Phe Val Gly 35 40 45 Ser Gly Ile Glu Gly Lys Leu Gly Lys Asn Lys Gly Glu Lys Lys Ile 50 55 60 Arg Lys Pro Lys Tyr Ala Phe Gln Thr Arg Ser Arg Val Asp Ile Leu 65 70 75 80 Asp Asp Gly Tyr Arg Trp Arg Lys Tyr Gly Gln Lys Ala Val Lys Asn 85 90 95 Asn Lys Phe Pro Arg Ser Tyr Tyr Arg Cys Thr His Gln Gly Cys Asn 100 105 110 Val Lys Lys Gln Val Gln Arg Leu Thr Lys Asp Glu Gly Val Val Val 115 120 125 Thr Thr Tyr Glu Gly Met His Ser His Gln Ile Glu Lys Ser Pro Asp 130 135 140 Asn Phe Glu His Ile Leu Ser Gln Met Gln Ile Tyr Ser 145 150 155 16475PRTPopulus tricocharpa 16Met Glu Ile Lys Glu Ser Glu Arg Val Val Ile Ala Lys Pro Val Ala 1 5 10 15 Ser Arg Pro Ser Tyr Ser Asn Phe Thr Ser Phe Ser Glu Leu Leu Ala 20 25 30 Gly Ala Ile Asn Thr Thr Pro Ser Asn Val Cys Ser Gly Thr Ala Val 35 40 45 Ala Ala Ile Arg Pro Lys Thr Val Arg Phe Arg Pro Leu Val Asn Arg 50 55 60 Ala Pro Gly Ala Leu Val Ser Ser Gln Ala Gly Leu Ser Gly Thr Ala 65 70 75 80 Val Ser Asn Ser Phe Asn Lys Ala Ser Ser Thr Asp Ser Lys Ala Thr 85 90 95 Ile Ile Tyr Lys Pro Gln Ala Lys Leu Val Ser Lys Ala Thr Val Ser 100 105 110 Leu Leu Ala Asn Met Gly Asn Phe Asn Thr Asn Ser Gln Gln Met Leu 115 120 125 Gln Pro Val Glu Thr Arg Pro Gln Leu Ser Lys Gln Asp Lys His Asn 130 135 140 Phe Ser Ser Gln Leu Thr Ser Asn Pro His Gln Asn Ile Pro Ser Pro 145 150 155 160 Ala Glu Ala Asp His Thr Thr Glu Pro Leu Arg Leu Thr Ser Leu Asn 165 170 175 Gln Glu Glu Asp Pro Lys Thr Leu Ser His Ala Ser Asn Gly Asp Arg 180 185 190 Pro Ser Tyr Asp Gly Tyr Asn Trp Arg Lys Tyr Gly Gln Lys Gln Val 195 200 205 Lys Gly Ser Glu Tyr Pro Arg Ser Tyr Tyr Lys Cys Thr Tyr Pro Asn 210 215 220 Cys Pro Val Lys Lys Lys Val Glu Arg Ser Phe Asp Gly Gln Ile Ala 225 230 235 240 Glu Ile Val Tyr Lys Gly Glu His Asn His Ser Lys Pro Gln Pro Pro 245 250 255 Lys Arg Asn Ser Ser Gly Thr Gln Gly Leu Ser Asp Gly Asn Ala Pro 260 265 270 Asp Arg Asn Ser Ile Pro Leu Trp Ser Asn Gln Leu Asn Glu Arg Asn 275 280 285 Glu Gly Ser Glu Gly Arg Glu Glu Asn Gln Asn Glu Ile Gly Leu Pro 290 295 300 Val His Ser Ile Tyr Gln Gly Lys Ala Pro Pro Ser Tyr Asp Pro Ala 305 310 315 320 Gly Thr Gly Thr Ile Asn Ala Gly Thr Gly Thr Ser Asp Asn Ser Cys 325 330 335 Gly Val Ser Gly Glu Cys Asp Asp Gly Ser Lys Gly Leu Glu Gly Ala 340 345 350 Asn Asp Glu Pro Lys Ser Lys Arg Arg Lys Thr Glu Ile Gln Ser Thr 355 360 365 Glu Gly Gly Met Ser Gly Glu Gly Val Gln Glu Pro Arg Val Val Val 370 375 380 Gln Ser Ser Thr Asp Ser Glu Ile Leu Gly Asp Gly Phe Arg Trp Arg 385 390 395 400 Lys Tyr Gly Gln Lys Ile Val Lys Gly Asn Pro Tyr Pro Arg Ser Tyr 405 410 415 Tyr Arg Cys Thr Ser Ile Lys Cys Asn Val Arg Lys His Val Glu Arg 420 425 430 Val Ser Asp Asp Pro Arg Ala Phe Ile Thr Thr Tyr Glu Gly Lys His 435 440 445 Asn His Glu Ile Pro Leu Lys Ser Thr Asn Leu Ala Ala Phe Glu Pro 450 455 460 Asp Ser Gln Ala Pro Thr Thr Arg Val Lys Gln 465 470 475 17355PRTPopulus tricocharpa 17Met Glu Glu Val Glu Glu Val His Lys Ala Ala Ile Glu Ser Cys Asn 1 5 10 15 Arg Val Ile Gly Leu Leu Cys Gln Gln Lys Asp Gln Val Gln Gly Arg 20 25 30 Asn Leu Met Val Glu Thr Arg Glu Thr Val Phe Lys Phe Lys Arg Val 35 40 45 Ile Ser Leu Leu Ser Thr Gly Leu Gly His Gly Arg Val Arg Lys Met 50 55 60 Lys Lys Leu Arg Pro Ser Leu Pro Gln Asn Ile Phe Leu Asp Ser Pro 65 70 75 80 Asn Cys Lys Thr Val Leu Ser Pro Lys Pro Leu Gln Met Val Pro Pro 85 90 95 Asn Phe Leu Glu Thr Pro Leu Thr Asp Met Asp Ala Lys Ser Lys Pro 100 105 110 Ser Ile Gln Ile Ser Gln Lys Met Leu Leu Glu Asn Pro Val Leu Glu 115 120 125 Leu Asn Ser Asn Ile Arg Pro Pro Val Gln Ile Met Gln Thr Lys Pro 130 135 140 Pro Gln Asn Phe Gln Leu Leu Gln Gln His Gln Gln Thr Gln Arg Met 145 150 155 160 His Phe Gln Gln Gln Gln Gln Gln Gln Met Lys Tyr Gln Ala Asp Arg 165 170 175 Val Tyr Ser Arg Ser Asn Gly Gly Ile Asn Leu Lys Phe Asp Gly Ser 180 185 190 Thr Cys Thr Pro Thr Met Ser Ser Thr Arg Ser Phe Ile Ser Ser Leu 195 200 205 Ser Met Asp Gly Ala Val Ser Thr Phe Asp Gly Asp Ser Phe His Leu 210 215 220 Ile Gly Met Pro His Ser Ser Asp His Ile Ser Gln Gln Thr Arg Arg 225 230 235 240 Arg Cys Ser Gly Arg Gly Glu Asp Gly Asn Ala Lys Cys Ser Ser Ser 245 250 255 Gly Lys Cys His Cys Ser Lys Arg Arg Lys Leu Arg Val Lys Arg Ser 260 265 270 Ile Lys Val Pro Ala Ile Ser Asn Lys Val Ala Asp Ile Pro Pro Asp 275 280 285 Glu Tyr Ser Trp Arg Lys Tyr Gly Gln Lys Pro Ile Lys Gly Ser Pro 290 295 300 His Pro Arg Gly Tyr Tyr Lys Cys Ser Ser Leu Arg Gly Cys Pro Ala 305 310 315 320 Arg Lys His Val Glu Arg Cys Leu Glu Asp Pro Ser Met Leu Ile Val 325 330 335 Thr Tyr Glu Gly Glu His Asn His Ser Arg Leu Ile Ser Ser Gln Ser 340 345 350 Ala His Thr 355 18718PRTPopulus tricocharpa 18Met Ala Gly Ile Asp Asp Asn Val Ala Ile Ile Gly Asp Trp Val Pro 1 5 10 15 Pro Ser Pro Ser Pro Arg Ala Phe Phe Ser Ala Met Leu Gly Asp Asp 20 25 30 Ile Asn Ser Arg Thr Ile Pro Glu Ser Pro Gly Glu Lys Arg Asn Glu 35 40 45 Gly Leu Tyr Leu Gly Gln Pro Glu Gln Met Ala Thr Gly Asn Ser Glu 50 55 60 Lys Lys Asp Gly Ala Gln Thr Ser Gly Val Gln Leu Thr Glu Leu Gly 65 70 75 80 Thr Phe Ser Glu Gln Lys Ser Asn Phe Arg Gly Gly Leu Val Glu Arg 85 90 95 Ile Ala Ala Arg Ala Gly Phe Asn Ala Pro Arg Leu Asn Thr Glu Ser 100 105 110 Ile Arg Ser Thr Glu Ser Ser Met Asn Pro Glu Ile Arg Ser Pro Tyr 115 120 125 Leu Thr Ile Pro Pro Gly Leu Ser Pro Thr Thr Leu Leu Glu Ser Pro 130 135 140 Val Phe Leu Ser Asn Leu Ala Gln Pro Ser Pro Thr Thr Gly Lys Phe 145 150 155 160 Ser Phe Phe Pro Asn Gly Asn Thr Lys Asn Ser Thr Val Gly Ser Asp 165 170 175 Pro Pro Asp Lys Ser Lys Glu Thr Phe Phe Asp Asn Ile Asp Ser Ser 180 185 190 Ser Phe Ala Phe Lys Pro Met Gly Glu Ser Gly Ser Phe Phe Leu Gly 195 200 205 Gly Thr Ser Lys Gln Ser Phe Pro Ser Ile Asp Val Ser Val His Ser 210 215 220 Glu Asn Ala Phe Gln Ser His Gly Val Glu Pro Ala Lys Thr Gln Phe 225 230 235 240 Glu Ser Arg Asn Ser Leu His Phe Arg Ala Glu Phe Ser Lys Leu Thr 245 250 255 Thr Glu Lys Asp Asn Gly Cys Ile Thr Val Ala Asp Gln Arg Thr Phe 260 265 270 Asp Thr Val Ala Gly Asn Ala Glu His Ser Ser Pro Leu Ala Glu Gln 275 280 285 Gln Asp Glu Glu Gly Asp Gln Arg Ala Ser Glu Asp Ser Met Ala Ala 290 295 300 Gly Gly Thr Pro Ser Glu Asp Ala Tyr Asn Trp Arg Lys Tyr Gly Gln 305 310 315 320 Lys Gln Val Lys Gly Ser Glu Tyr Pro Arg Ser Tyr Tyr Lys Cys Thr 325 330 335 His Pro Asn Cys Pro Val Lys Lys Lys Val Glu Arg Ser His Glu Gly 340 345 350 His Ile Thr Glu Ile Ile Tyr Lys Gly Ala His Asn His Pro Lys Pro 355 360 365 Pro Pro Asn Arg Arg Ser Ala Thr Gly Ser Met Asp Thr Gln Leu Asp 370 375 380 Ile Pro Glu Gln Ala Gly Pro Gln Val Gly Ala Val Asn Asp Ser Val 385 390 395 400 Trp Ala Gly Thr Gln Lys Gly Thr Ala Ala Gly Thr Pro Asp Trp Arg 405 410 415 Asn Asp Asn Val Glu Val Ser Ser Ser Ala Ser Gly Gly Leu Gly Pro 420 425 430 Glu Phe Gly Asn Pro Ser Ser Ser Val Gln Gly Gln Ser Gly Thr Pro 435 440 445 Phe Glu Ser Ala Asp Ala Val Asp Ala Ser Ser Thr Phe Ser Asn Asp 450 455 460 Glu Asp Asp Asp Arg Ala Thr His Gly Ser Val Gly Tyr Asp Gly Glu 465 470 475 480 Gly Glu Glu Ser Glu Ser Lys Arg Arg Lys Val Glu Thr Tyr Ala Thr 485 490 495 Glu Met Ser Gly Ala Thr Arg Ala Ile Arg Glu Pro Arg Val Val Val 500 505 510 Gln Thr Thr Ser Glu Val Asp Ile Leu Asp Asp Gly Tyr Arg Trp Arg 515 520 525 Lys Tyr Gly Gln Lys Val Val Lys Gly Asn Pro Asn Pro Arg Ser Tyr 530 535 540 Tyr Lys Cys Thr Ser Ala Gly Cys Thr Val Arg Lys His Val Glu Arg 545 550 555 560 Ala Ser His Asp Leu Lys Ser Val Ile Thr Thr Tyr Glu Gly Lys His 565 570 575 Asn His Asp Val Pro Ala Ala Arg Asn Ser Asn His Val Asn Ser Gly 580 585 590 Thr Ser Asn Ala Thr Pro Ala Gln Ala Gly Ile Ala Val Gln Thr Gln 595 600 605 Val His Arg Pro Glu Ala Ser Gln Val His Asn Ser Met Thr Ser Arg 610 615 620 Phe Glu Arg Pro Pro Ala Phe Gly Ser Phe Arg Gln Gln Leu Gly Pro 625 630 635 640 Ser Ala Gly Phe Ser Phe Gly Met Asn Gln Pro Gly Phe Ala Asn Met 645 650 655 Gly Met Ala Gly Leu Gly Pro Gly Gln Pro Arg Met Pro Val Met Pro 660 665 670 Met His Pro Tyr Leu Ala Gln Gln Arg Ala Val Asn Glu Met Gly Leu 675 680 685 Met Met Pro Lys Gly Glu Ala Lys Val Glu Pro Val Thr Ser Asn Asn 690 695 700 Pro Thr Val Tyr Gln Gln Ile Met Ser Arg Leu Pro Gln Met 705 710 715 19301PRTPopulus tricocharpa 19Met Ala Val Asp Leu Val Arg Tyr Ser Lys Met Glu Asp Gln Met Ala 1 5 10 15 Ile Gln Glu Ala Ala Ser Ala Gly Leu Glu Ser Met Glu His Leu Ile 20 25 30 Phe Ala Phe Ser Asn Gln Thr Arg Gln Ser His Gln Leu Asp Cys Gly 35 40 45 Glu Ile Thr Asn Phe Thr Val Ala Lys Phe Lys Gln Val Ile Ser Met 50 55 60 Leu Asn Arg Thr Gly His Ala Arg Phe Arg Arg Gly Pro Thr Ser Ser 65 70 75 80 Pro Ser Ser Tyr Pro Val Pro Val Arg Pro Val Pro

Gln Glu Pro Gln 85 90 95 Lys Leu Asn Leu Asp Phe Val Asn Ser Asn Ser Pro Pro Lys Ala Glu 100 105 110 Ser Lys Asn Asp Leu Ser Leu Gly Ser Gln Tyr Ser Lys Asp Ser Leu 115 120 125 Ser Ser Gly Thr Thr Thr Ser Ser Phe Val Ser Ser Val Thr Ala Asp 130 135 140 Gly Ser Val Ser Asn Gly Lys Gln Gly Gly Ser Ser Leu Phe Gly Thr 145 150 155 160 Gln Ala Arg Ser Thr Gly Lys Pro Pro Leu Ser Ser Thr His Arg Lys 165 170 175 Lys Cys His Asp His Ala Leu Ser Ala Arg Lys Ile Ser Ser Gly Gly 180 185 190 Ser Cys His Cys Ser Lys Arg Arg Lys Ser Arg Val Lys Arg Thr Ile 195 200 205 Arg Val Pro Ala Val Ser Ser Lys Ile Ala Asp Ile Pro Ala Asp Glu 210 215 220 Tyr Ser Trp Arg Lys Tyr Gly Gln Lys Pro Ile Lys Gly Ser Pro Tyr 225 230 235 240 Pro Arg Gly Tyr Tyr Lys Cys Ser Ser Val Arg Gly Cys Pro Ala Arg 245 250 255 Lys His Val Glu Arg Ala Val Asp Asp Ser Ala Met Leu Ile Val Thr 260 265 270 Tyr Glu Gly Glu His Arg His Ser His Thr Pro Leu Pro Gly Asp Val 275 280 285 Thr Ala Ser Ala Ala Met Arg His Val Phe His Ser Thr 290 295 300 20347PRTPopulus tricocharpa 20Met Glu Gly Val Glu Glu Ala Asn Arg Ala Ala Val Glu Ser Cys His 1 5 10 15 Arg Val Ile Ser Leu Leu Ser Gln Pro Gln Asp Gln Val Gln Tyr Arg 20 25 30 Asn Leu Met Val Glu Thr Gly Glu Ala Val Phe Arg Phe Lys Lys Val 35 40 45 Val Ser Leu Leu Asn Thr Gly Leu Gly His Ala Arg Val Arg Lys Leu 50 55 60 Lys Lys Leu Pro Thr Pro Leu Ser Gln Ser Ile Leu Leu Asp Asn Pro 65 70 75 80 Leu Ser Ser Thr Asp His Pro Ser Lys Thr Pro Gln Phe Leu Gln Ser 85 90 95 Ser Ser Tyr Leu Glu Ser Gln Pro Ile Gln Glu Leu Gly Ser Ile Ala 100 105 110 Lys Asn Cys Leu Ser Leu Gly Thr Pro Ser Leu Glu Leu Ser Ser Asn 115 120 125 Gly Lys Asn Pro Leu Gln Leu Gly Gln Pro Thr Pro Ala Ala His Tyr 130 135 140 Gln Phe Leu Gln Gln Gln Gln Leu His Arg Leu Gln Leu Gln Gln Gln 145 150 155 160 Gln Gln Met Lys Gln Gln Ala Glu Met Met Phe Arg Lys Ser Asn Ser 165 170 175 Gly Ile Ser Leu Asn Phe Asp Ser Ser Ser Cys Thr Pro Thr Met Ser 180 185 190 Ser Thr Arg Ser Phe Ile Ser Ser Leu Ser Ile Asp Gly Asn Val Ala 195 200 205 Asn Leu Glu Gly Ser Ala Phe His Leu Thr Gly Ala Ala Arg Ser Ser 210 215 220 Asp Gln Ser Ser Gln Gln His Lys Arg Lys Cys Ser Gly Arg Gly Glu 225 230 235 240 Asp Gly Ser Met Lys Cys Gly Ser Ser Val Arg Cys His Cys Ser Lys 245 250 255 Lys Arg Lys His Arg Val Lys Arg Ser Ile Lys Val Pro Ala Ile Ser 260 265 270 Asn Lys Leu Ala Asp Ile Pro Pro Asp Asp Tyr Ser Trp Arg Lys Tyr 275 280 285 Gly Gln Lys Pro Ile Lys Gly Ser Pro His Pro Arg Gly Tyr Tyr Lys 290 295 300 Cys Ser Ser Met Arg Gly Cys Pro Ala Arg Lys His Val Glu Arg Cys 305 310 315 320 Leu Glu Asp Pro Ser Met Leu Ile Val Thr Tyr Glu Gly Glu His Asn 325 330 335 His Pro Arg Ile Pro Ala Gln Ser Thr Asn Thr 340 345 21365PRTPopulus tricocharpa 21Met Glu Leu Ser Met Glu Trp Glu His Lys Thr Leu Ile Ser Glu Leu 1 5 10 15 Thr Gln Gly Lys Glu Leu Ala Lys Gln Leu Ser Asn His Leu Asn Pro 20 25 30 Ser Ser Ser Leu Glu Ala Arg Gln Phe Leu Val Asp Lys Ile Leu Ser 35 40 45 Ser Tyr Glu Lys Ala Leu Ser Met Leu Asn Trp Gly Ala Leu Ala Ala 50 55 60 Asp Gln Pro Lys Pro Thr Ile Gly Thr Val Glu Pro Leu His Ser Phe 65 70 75 80 Ala Asn Ser Ser Pro Arg Ser Glu Val Ser Asp Gln Asp Cys Lys Glu 85 90 95 Glu Cys Asn Lys Asp Val Tyr Lys Lys Arg Lys Ile Gln Pro Arg Trp 100 105 110 Thr Glu Gln Val Lys Asp Cys Ser Gly Thr Gly Leu Glu Gly Pro Leu 115 120 125 Asp Asp Gly Tyr Ser Trp Arg Lys Tyr Gly Gln Lys Asp Ile Leu Gly 130 135 140 Ala Asn Phe Pro Arg Gly Tyr Tyr Arg Cys Thr His Arg His Ser Gln 145 150 155 160 Gly Cys Leu Ala Thr Lys Gln Val Gln Arg Ser Asp Glu Asp His Ser 165 170 175 Ile Phe Glu Val Thr Tyr Arg Gly Arg His Thr Cys Asn Gln Ala Ser 180 185 190 Pro Ser Pro Val Ala Ser Pro Ser Pro Lys Asn Asp Cys Ser Lys Gln 195 200 205 Ser Lys Tyr His Arg Lys Gln Gln Gln Gln Arg Gln Glu Lys Pro Lys 210 215 220 Pro Thr Glu Glu Ile Phe Val Asn Phe Gly Ser Asp Ile Val Gln Val 225 230 235 240 Lys Asn Glu Asp Leu Gly Ser Lys Asp Asp Ile Phe Pro Ser Phe Ser 245 250 255 Phe Pro Cys Thr Ser Phe Gly Asn Glu Asn Glu Glu Asn Asn Ile Phe 260 265 270 Thr Glu Ser Met Met Glu Asn Asn Phe Leu Gly Ser Phe Ser Pro Thr 275 280 285 Phe Ile Ser Pro Ala Thr Ser Glu Ser Asn Tyr Phe Ser Met Ser Pro 290 295 300 Cys His Met Asn Ser Phe Gly Ile Gly Tyr Gln Asn Val Gln Thr Pro 305 310 315 320 Glu Ser Glu Leu Thr Thr Glu Ile Ile Ser Ala Pro Thr Ser Val Thr 325 330 335 Asn Ser Pro Ile Gly Asp Phe Asp Ile Ser Ile Asp Asn Val Asp Phe 340 345 350 Asp Thr Thr Phe Pro Phe Asp Asn Pro Asp Phe Phe Ala 355 360 365 22357PRTPopulus tricocharpa 22Met Met Glu Glu Leu Tyr Gly Leu Gln Ser Thr Ser Ser Asp Tyr Ser 1 5 10 15 Leu Gln Val Pro Ser Glu Asn Met Val Ser Pro Val Ala Asn Tyr Tyr 20 25 30 His Pro Val Gly Phe Thr Ser Pro Ala Gly Glu Pro Ser Phe Pro Val 35 40 45 Phe Gly Ser Glu Gln Met Phe Cys Gly Ser Ser Val Ser Asp Ala Ala 50 55 60 Ser Met Gly Ala Glu Leu His Gln Lys Gln Gln Gln Gln Ser Gly Gly 65 70 75 80 Val Gly Val Gly Asp His Ser Asn Arg Asn Asn Ser Glu Glu Val Ser 85 90 95 Cys Ala Ile Arg Ala Lys Ile Ala Ser His Pro Leu Tyr Pro Lys Leu 100 105 110 Leu Glu Ala Tyr Ile Asp Cys Gln Lys Val Gly Ala Pro Pro Glu Met 115 120 125 Ala Tyr Leu Leu Asp Glu Ile Arg Leu Val Asn Asp Val Ser Lys Gly 130 135 140 Ser Asn Asp Thr Val Ala Ser Cys Leu Gly Ala Asp Pro Glu Leu Asp 145 150 155 160 Glu Phe Met Glu Thr Tyr Cys Asp Val Leu Met Lys Tyr Lys Ala Asp 165 170 175 Leu Ser Arg Pro Phe Asp Glu Ala Thr Thr Phe Leu Asn Asp Ile Glu 180 185 190 Ala Gln Phe Asn Thr Leu Cys Asn Gly Pro Ser Arg Ser Gln Val Tyr 195 200 205 Gly Leu Pro Leu Ser Leu Ser Leu Ser Ile Leu Leu Leu Ser Met Leu 210 215 220 Ser Asn Glu Ala Ala Gly Ser Ser Asp Glu Asp Ala Ser Gly Gly Glu 225 230 235 240 Ala Gly Met Gln Asp Ser Thr Arg Ile Asn Glu Asp Arg Glu Leu Lys 245 250 255 Asp Lys Leu Leu Arg Lys Tyr Ser Gly Tyr Ile Ser Thr Leu Lys His 260 265 270 Ala Phe Ser Lys Gln Lys Lys Lys Gly Lys Leu Pro Lys Glu Ala Arg 275 280 285 Gln Ile Leu Leu Asn Trp Trp Asn Ile His Asn Lys Trp Pro Tyr Pro 290 295 300 Thr Glu Ala Asp Lys Val Ala Leu Ala Glu Ser Thr Gly Leu Asp Gln 305 310 315 320 Lys Gln Ile Asn Asn Trp Phe Ile Asn Gln Arg Lys Arg His Trp Lys 325 330 335 Pro Ser Glu Asn Met Gln Phe Ala Val Val Asp Ser Leu Tyr Gly Pro 340 345 350 Phe Phe Met Asn Asp 355 23568PRTPopulus tricocharpa 23Met Glu Arg Asp Lys Leu Phe Val Ser Glu Gly Ala Asn Thr Ala Ala 1 5 10 15 Thr Ile Trp Asn Ser Cys Ser Phe Gly Met Glu Met Gln Ala Asn Glu 20 25 30 Leu Ser Cys Gly Pro Glu Lys Leu Ala Asn Cys Phe Leu Asn Pro Asn 35 40 45 Trp Asp Asn Ser Leu Asp Gln Ser Asp Pro Phe Glu Ser Ala Leu Ser 50 55 60 Ser Ile Val Ser Ser Pro Val Ala Ser Gly Ala Asn Ala Asn Ala Asn 65 70 75 80 Ala Ile Pro Asn Ala Gly Val Gly Gly Asp Ser Leu Met Ile Arg Glu 85 90 95 Leu Ile Gly Arg Leu Gly Asn Ile Cys Asn Ser Gly Asp Ile Ser Leu 100 105 110 Gln Ser Phe Val Asn Asn Asn Asn Asn Ser Thr Asn Thr Ser Cys Tyr 115 120 125 Ser Thr Pro Met Asn Ser Pro Pro Lys Leu Asn Leu Ser Met Met Asp 130 135 140 Ser Gln Met Arg Gly Asn Leu Pro Ile Pro Gly Asn Ser Val Val Lys 145 150 155 160 His Pro Gly Leu Ala Pro Phe Pro Ala Asp Phe Val Glu Arg Ala Ala 165 170 175 Arg Tyr Ser Cys Phe Gly Ser Asn Asn Pro Gly Gly Ile Asn Lys Gln 180 185 190 Phe Gly Leu Asn Glu Ser Glu Leu Ile Asn Arg Leu Met Pro Arg Val 195 200 205 Glu Pro Gly Lys Leu Ser Arg Val Ser Ser Asn Asn Ser Met Lys Val 210 215 220 Thr Val Ser Gln Ala Asn Val Gln Glu Ser Asn Lys Ser Ser Pro Gln 225 230 235 240 Asp Gly Ser Leu Asn Ser Glu Lys Lys Phe Ser Arg Gln Ser Arg Pro 245 250 255 Thr Thr Ser Glu Asn Gly Asp Ser Arg Glu Glu Ser Ser Leu Ser Glu 260 265 270 Gln Val Pro Gly Gly Lys Leu Ser Met Lys Ser Gln Asn Asp Ala Asn 275 280 285 Ser Arg Lys Arg Lys Ser Ile Pro Arg Gly Lys Ala Lys Glu Thr Pro 290 295 300 Ser Ser Ser Pro Ser Ala Ser Asp Val Lys Val Ala Ala Glu Asn Asp 305 310 315 320 Glu Ser Lys Ala Lys Arg Ser Lys Ser Asp Glu Thr Asn Gly Ser Asp 325 330 335 Lys Asp Thr Ala Lys Glu Lys Glu Glu Glu Asn Gly Asn Gln Lys Gln 340 345 350 Asn Lys Asn Asn Ser Lys Pro Pro Glu Pro Pro Lys Asp Tyr Ile His 355 360 365 Val Arg Ala Arg Arg Gly Gln Ala Thr Asp Ser His Ser Leu Ala Glu 370 375 380 Arg Val Arg Arg Glu Lys Ile Ser Glu Arg Met Lys Phe Leu Gln Asp 385 390 395 400 Leu Val Pro Gly Cys Asn Lys Val Thr Gly Lys Ala Val Met Leu Asp 405 410 415 Glu Ile Ile Asn Tyr Val Gln Ser Leu Gln Arg Gln Val Glu Phe Leu 420 425 430 Ser Met Lys Leu Ser Ser Val Asn Pro Arg Met Glu Ile Asn Met Glu 435 440 445 Thr Leu Leu Ser Lys Asp Ile Phe Gln Ser Arg Gly Ser Met Pro His 450 455 460 Ser Leu Tyr Pro Leu Asp Ala Ser Thr Pro Val Phe Pro Tyr Gly Tyr 465 470 475 480 Gln Ser Gln Gln Gly Leu Ala Leu Gln Asn Gly Met Pro Ser Asn Ala 485 490 495 Glu Thr Gln Phe Ser Met Asn Pro Leu Asn Ala Ala Leu Arg Arg Asn 500 505 510 Pro Ser Met His Leu Pro His Leu Asp Gly Phe Gly Asp Pro Ala Ala 515 520 525 Leu Gln Ala Ser Ala Met Trp Glu Asp Asp Leu Gln Ser Val Val Gln 530 535 540 Met Gly Tyr Gly Gln Asn His Gln Glu Ser Phe Gln Gly Ser Val Pro 545 550 555 560 Ser Thr His Met Lys Ile Glu Leu 565 24391PRTPopulus tricocharpa 24Met Ser Val Gly Val Asn Thr Glu Thr Leu Val Arg Val Glu Glu Ile 1 5 10 15 Gln Gly Arg Gly Arg Gly Leu Val Ser Thr Gln Pro Leu Arg Gly Gly 20 25 30 Gln Ile Val Leu Ile Asp Ser Pro Ile Leu Leu Tyr Ser Ala Leu Pro 35 40 45 Leu Thr Lys Gln Gln His Ser Thr Phe Leu Tyr Cys Asp Lys Cys Phe 50 55 60 Lys Thr Ile Gln Ser Ala Ser Val Ser Cys Pro Thr Cys Ser His Gln 65 70 75 80 Arg Phe Cys Ser Pro Thr Cys Leu Ser Ala Ala Leu Ala Ser Ser His 85 90 95 Thr Pro Trp Val Cys Gln Ser Leu Ser Arg Leu Arg Asp Cys Gln Asp 100 105 110 Phe Leu Gln His His Ser Val Glu Arg Gln Ile Gln Ala Gln Phe Leu 115 120 125 Val Ala Ala Tyr Asn Leu Ala Phe Val Ser Pro Ser Asp Phe Gln Ile 130 135 140 Leu Leu Ser Leu Gln Gly Arg Ala Glu Asp Glu Asp Pro Ala Ile Val 145 150 155 160 Gln Ser Leu His Ser Val Ile Ser Ser Leu Cys Pro Pro Pro Pro Ile 165 170 175 Glu Gly Phe Ser Phe Ser Leu Glu Leu Ile Ala Ala Leu Val Ala Lys 180 185 190 Asp Arg Phe Asn Ala Phe Gly Leu Met Glu Pro Leu Asn Leu Asn Glu 195 200 205 Glu Asn Gly Gly Gln Arg Ser Val Arg Ala Tyr Gly Ile Tyr Pro Lys 210 215 220 Ala Ser Leu Phe Asn His Asp Cys Leu Pro Asn Ala Cys Arg Phe Asp 225 230 235 240 Tyr Val Asp Thr Asn Asn Ser Gly Asn Thr Asp Ile Val Val Arg Met 245 250 255 Ile His Asp Val Pro Gln Gly Arg Glu Ile Cys Leu Ser Tyr Phe Pro 260 265 270 Val Asn Ser Asn Tyr Ser Thr Arg Arg Lys Arg Leu Leu Glu Asp Tyr 275 280 285 Gly Phe Thr Cys Asp Cys Asp Arg Cys Lys Val Glu Ala Thr Trp Ser 290 295 300 Asp Asp Glu Gly Asp Gly Asp Asp Asn Asp Asn Glu Val Met Glu Glu 305 310 315 320 Asp Val Asp Glu Pro Met Glu Ala Glu Ser Asp Gly Glu Glu Ile Gly 325 330 335 Asn Asp Asn Ser Thr Asp Phe Pro His Ala Tyr Phe Phe Leu Arg Tyr 340 345 350 Met Cys Asn Arg Asn Asn Cys Trp Gly Thr Leu Ala Pro Phe Pro Pro 355 360 365 Ser Asp Ala Lys Pro Ser Asn Leu Leu Glu Cys Asn Ala Cys Gly Asp 370 375 380 Ile Lys Asn Asp Glu Val Cys 385 390 25335PRTPopulus tricocharpa 25Met Tyr Gln Leu Glu Ser Val Pro Ser Ser Ser Ser Val His Lys Asn 1 5 10 15 Leu Leu Val Asn Asp Gln Tyr Leu Asp Cys Asp Asp Met Thr Met Asp 20 25 30 Pro Ile Asn Gly Gly Asn Asn Leu Asn Asn Asn Pro Asn Leu Ala Ser 35

40 45 Lys Gln Arg Leu Arg Trp Thr His Glu Leu His Glu Arg Phe Val Asp 50 55 60 Ala Val Ala Gln Leu Gly Gly Pro Asp Arg Ala Thr Pro Lys Gly Val 65 70 75 80 Leu Arg Val Met Gly Val Gln Gly Leu Thr Ile Tyr His Val Lys Ser 85 90 95 His Leu Gln Lys Tyr Arg Leu Ala Lys Tyr Leu Pro Asp Ser Ser Ser 100 105 110 Asp Gly Lys Lys Ala Asp Lys Lys Glu Thr Gly Asp Met Ile Ser Asn 115 120 125 Leu Asp Gly Ser Ser Gly Met Gln Ile Thr Glu Ala Leu Lys Leu Gln 130 135 140 Met Glu Val Gln Lys Arg Leu His Glu Gln Leu Glu Val Gln Arg Gln 145 150 155 160 Leu Gln Leu Arg Ile Glu Ala Gln Gly Lys Tyr Leu Lys Lys Ile Ile 165 170 175 Glu Glu Gln Gln Arg Leu Ser Gly Val Leu Glu Asp Val Pro Gly Ser 180 185 190 Gly Val Thr Ala Pro Val Ser Gly Asp Asn Cys Pro Glu Ser Asp Lys 195 200 205 Thr Asp Pro Ala Thr Pro Ala Pro Thr Ser Glu Ser Pro Leu Gln Asp 210 215 220 Lys Ala Ala Lys Glu Arg Ala Pro Ala Lys Ser Leu Ser Ile Asp Glu 225 230 235 240 Ser Phe Ser Ser Gln Pro Glu Pro Leu Thr Pro Asp Ser Arg Cys Asn 245 250 255Ala Gly Ser Pro Ala Glu Ser Pro Arg Gly Glu Arg Ser Met Lys Lys 260 265 270 Gln Arg Val Ser Met Gly Val Thr Tyr Gly Lys Gln Glu Met Val Leu 275 280 285 Thr His Gln Ile Leu Glu Ser Ser Leu Asn Ser Tyr Pro Arg Pro His 290 295 300 Ser Ala Phe Leu Gly Arg Glu Gln Phe Asp Pro Ser Ser Gly Leu Ser 305 310 315 320 Met Gly Ile Glu Asp Gln Met Glu Lys Val Ser Gly Ser Asp Val 325 330 335 26366PRTPopulus tricocharpa 26Met Ser Pro Pro Leu Leu Gly Val Val Glu Glu Glu Gly His Ser Asn 1 5 10 15 Val Thr Leu Leu Ala Ser Pro Ala Ser Ala Glu Ser Ala Cys Leu Asn 20 25 30 Gly Leu Glu Leu Lys Glu Arg Asn Tyr Met Gly Leu Ser Asp Cys Ser 35 40 45 Ser Val Asp Ser Ser Ala Val Ser Ala Ala Ser Asp Glu Arg Lys Thr 50 55 60 Ser Leu Asn Leu Lys Ala Thr Glu Leu Arg Leu Gly Leu Pro Gly Ser 65 70 75 80 Gln Ser Pro Glu Arg Asn His Glu Leu Ser Leu Leu Ser Ser Ala Leu 85 90 95 Leu Asp Glu Lys Pro Phe Phe Pro Leu His Pro Ser Asn Asp Gly His 100 105 110 Tyr Ser Ser Thr Gln Lys Asn Val Val Ser Gly Asn Lys Arg Val Phe 115 120 125 Ser Asp Ala Met Asp Glu Phe Ser Glu Ser Lys Phe Leu Ser Asn Ser 130 135 140 Glu Val Asn Ala Met Leu Ser Pro Arg Pro Ser Pro Asn Met Gly Leu 145 150 155 160 Lys Pro Gly Met Leu Glu Asn Leu Gly Val Gln Gln Ala Lys Val Lys 165 170 175 Glu Ile Val Ala Pro Lys Ala Gly Gln Glu Arg Pro His Ala Ala Asn 180 185 190 Glu Thr Arg Pro Leu Arg Asn Ser Ser Ala Asn Asn Ser Ser Ala Pro 195 200 205 Ala Pro Lys Ala Gln Val Val Gly Trp Pro Pro Ile Lys Ser Phe Arg 210 215 220 Lys Asn Ser Leu Ala Thr Thr Ser Lys Asn Thr Glu Glu Val Asp Gly 225 230 235 240 Lys Ala Gly Pro Gly Ala Leu Phe Ile Lys Val Ser Met Asp Gly Ala 245 250 255 Pro Tyr Leu Arg Lys Val Asp Leu Arg Asn Tyr Ser Ala Tyr Gln Glu 260 265 270 Leu Ser Ser Ala Leu Glu Lys Met Phe Ser Cys Phe Thr Ile Gly Gln 275 280 285 Tyr Gly Ser His Gly Ala Pro Gly Arg Glu Met Leu Ser Glu Ser Lys 290 295 300 Leu Lys Asp Leu Leu His Gly Ser Glu Tyr Val Leu Thr Tyr Glu Asp 305 310 315 320 Lys Asp Gly Asp Trp Met Leu Val Gly Asp Val Pro Trp Glu Met Phe 325 330 335 Ile Glu Thr Cys Lys Arg Leu Arg Ile Met Lys Ser Ser Asp Ala Ile 340 345 350 Gly Leu Ala Pro Arg Ala Met Glu Lys Cys Lys Asn Arg Asn 355 360 365 2721DNAArtificialForward cloning primer 27atggctgtgg agcttatgat g 212828DNAArtificialForward cloning primer 28atgtctgtga acatgatcaa ctcttatg 282924DNAArtificialForward cloning primer 29atggagatca aggaatcaga gagg 243022DNAArtificialForward cloning primer 30atggaggagg ttgaggaggt ac 223122DNAArtificialForward cloning primer 31atggctggga ttgatgataa cg 223226DNAArtificialForward cloning primer 32atggctgtgg atctagttag gtattc 263320DNAArtificialForward cloning primer 33atggaggggg ttgaagaagc 203422DNAArtificialForward cloning primer 34atggaattgt ccatggaatg gg 223525DNAArtificialForward cloning primer 35atgatggaag aactttacgg tcttc 253626DNAArtificialForward cloning primer 36atggaaagag ataagttgtt tgtgag 263726DNAArtificialForward cloning primer 37atgtctgttg gtgtaaacac agaaac 263822DNAArtificialForward cloning primer 38atgtatcagc tggagagcgt tc 223925DNAArtificialForward cloning primer 39atgtctccac cactacttgg tgttg 254022DNAArtificialReverse cloning primer 40agtgtcttgt cttacacgga tg 224121DNAArtificialReverse cloning primer 41tggcgtttga ggcttaattt g 214221DNAArtificialReverse cloning primer 42cggcagcaaa gagaaaagat g 214323DNAArtificialReverse cloning primer 43gtttcatgta tgcgcagact gtg 234425DNAArtificialReverse cloning primer 44attttacatc tgaggcagcc tactc 254524DNAArtificialReverse cloning primer 45tcatgttgag tgaaacacgt gtcg 244624DNAArtificialReverse cloning primer 46gggtggttac gtgtttgtgg attg 244724DNAArtificialReverse cloning primer 47ttaagcaaag aaatctgggt tgtc 244820DNAArtificialReverse cloning primer 48catcagcatg aggccctgtc 204922DNAArtificialReverse cloning primer 49gggatcaggg actgtttatt gg 225024DNAArtificialReverse cloning primer 50cagcatcaac aaacctcatc attc 245125DNAArtificialReverse cloning primer 51ctaaacatca ctgcctgaaa ctttc 255225DNAArtificialReverse cloning primer 52ctaattcctg tttttgcatt tctcc 255320DNAArtificialForward real-time RT PCR primer 53cagaaccagg accgcaacag 205420DNAArtificialForward real-time RT PCR primer 54atggtgccca attcttatgc 205521DNAArtificialForward real-time RT PCR primer 55aagaaatgaa ggctctgaag g 215622DNAArtificialForward real-time RT PCR primer 56attggtatgc ctcactcatc tg 225721DNAArtificialForward real-time RT PCR primer 57gcagatggca acaggaaatt c 215821DNAArtificialForward real-time RT PCR primer 58gactgcggag aaatcacaaa c 215923DNAArtificialForward real-time RT PCR primer 59cattttccca tccttttcct tcc 236022DNAArtificialForward real-time RT PCR primer 60tagtgaatga tgtttccaag gg 226118DNAArtificialForward real-time RT PCR primer 61agccaacaca gcagcaac 186222DNAArtificialForward real-time RT PCR primer 62cactatgtcc tctacttcct tg 226320DNAArtificialReverse real-time RT PCR primer 63tgaggcaaag gagggagacg 206423DNAArtificialReverse real-time RT PCR primer 64ctagttatag acccaacaaa gcc 236521DNAArtificialReverse real-time RT PCR primer 65gctgacacca catgagttat c 216621DNAArtificialReverse real-time RT PCR primer 66atctcttcac cctcagtttc c 216721DNAArtificialReverse real-time RT PCR primer 67tcaacaagac ctccacgaaa g 216823DNAArtificialReverse real-time RT PCR primer 68taggaagaag gagaagaagt tgg 236923DNAArtificialReverse real-time RT PCR primer 69agtagttgga ttcagatgtt gcc 237023DNAArtificialReverse real-time RT PCR primer 70ttgagagatc cgctttatac ttc 237119DNAArtificialReverse real-time RT PCR primer 71tttctctgga ccgcaactc 197219DNAArtificialReverse real-time RT PCR primer 72atggtcttgg gtcttcctg 19731154DNAPopulus tremula x Populus tremuloides 73atggctgtgg agcttatgat ggggtattct ggcgatagtt ttgctacaaa aatgcaagag 60aatgatgtga gagaagccgc aactgctggg atacaaagcg ttgaggaagt cataaaactg 120ctcaaacaaa atcaactgga acagcaacaa aaacaacaat actaccagga gttgtctgca 180gcctcctcaa gttccaatct tggcacggat aatatcatgg ctgttactga tatggccgtg 240aacaatttca aaaaggttat ttctttactg ggtcgtacca caagaactgg ccatgctcga 300tttagaagag ctcctgttgc ctgccctcct caacaacgaa tgcaagaacc agaaccagaa 360ccgcaacagc aaaaacagca agttcaagag ccagtaccat atgttcgagc aattaattcg 420cagccaacag agcaaggctc tgcttttaga gtttatcaac cgaccccaat tcatcgtctc 480cctcctttgc ctcacaatca gcaacaaaag acactggtgg ttacgaaaaa tggattatca 540gatcggaatg aaatggctac tacgatcaat ttctccaatt cgccaacaat atctgctcct 600acttctttct tgtcttctgt aacaggggaa actgatagct tccagcgttc tacgctttct 660gggtttcagt ttacccaacc ttctgctggt aaaccccctt tgtcctcttc ttctcttaag 720agaaagtgta actccatgga tgatgctgct ctcaagtgtg gctcctcttc tagtcgctgc 780cactgctcca agaaaaggaa atcaagaatt aaaagggtgg ttagagttcc tgcaattagt 840agtaagatgg ctgatatccc acctgatgat tattcctgga gaaagtatgg tcaaaagccc 900atcaaaggct ctcctcatcc caggggatac tacaagtgca gtagcgtgag aggatgtccg 960gcacgcaaac acgtggagag agctctagat gactcgatga tgcttgttgt gacctatgaa 1020ggggaacaca accactctca tccaatcgat gaagcacccg gtgctcttgt ccttgaatca 1080tcttaagtca actgatcaat cgacatggaa aatatcttcc ccggttcatt cacatccgtg 1140taagacaaga cact 115474595DNAPopulus tremula x Populus tremuloides 74atgtctgtga acatgatcaa ctcttatgct tctaccgact ttgaaggtgc aaggggaaac 60ggattgttgg gattgatgca agagatgcaa ggtccagcca gtttgaattt tgatgacatc 120tctcaaaaca gaggctttgt tgggtctgga actgaggaga aattgggaaa gaataaggga 180ggaaagaaga taagaaagcc caagtacgct tttcaaacaa ggagccgagt tgatatcctt 240gatgatggat atcgatggag aaagtatggt caaaaagcag tgaagaacaa caaatttccc 300agaagctact atcggtgtac acatcaaggg tgcgacgtga agaagcaggt tcagagatta 360acaaaagacg aaggagtcgt ggtgacaacc tatgaaggaa tgcattctca tcagatcgaa 420aagtctccag ataacttcga gcatatcttg agccagatgc aaatctactc ctaatccttc 480taattaagtc cctagctagg cctctatatc atcgttacat aaggaaatta tggtatcgag 540atatccgtag actcttacat caataaacta tagacaaatt aagcctcaaa cgcca 59575906DNAPopulus tremula x Populus tremuloides 75atggctgtgg atctagttag gtattcaaag atggaagatc agatggctat acaagaagct 60gcatcagctg ggctcgagag catggagcac ttgatctttg cactctctaa ccaaactcga 120caaagccacc aacttgactg cggagaaatc acaaacttca ccgttgccaa gttcaagcaa 180gtcatctcca tgttgaaccg gaccggccat gcccgttttc gccgtggacc aacttcttct 240ccttcttcct acccggttcc cgtccgacct gtccctcaag aacctcaaaa actgaacctt 300gattttgtta acagtaagag cccccctaaa gctgagtcga aaaatgacct gtctttgggt 360agtcagtatt caaaggatag ccttagctct ggcaccacta cctcatcctt cgtgtcttct 420gttacagctg atgggagtgt ctctaatggg aaacaaggtg gctcttctct tttcggaact 480caagctcgat ctaccggaaa gccacctctc tcatcgaccc accgcaagaa atgccacgat 540catgcccttt ccgccagaaa gatctcctcc ggtggcagct gtcattgctc caaaagaagg 600aaatcaaggg ttaagaggac aataagggta ccagccgtga gttccaagct tgccgatata 660ccagcagatg agtactcatg gagaaaatat ggtcaaaagc caatcaaggg ctcaccatac 720ccaagagggt attacaagtg tagtagtgtg aggggatgtc ccgcaaagaa gcatgtggag 780cgtgccgtag atgactcggc catgcttatt gtgacttacg agggggagca ccgtcactca 840catactccgt tgccggaaga cgtcacggct agtgctgcaa tgcgacacgt gtttcactca 900acatga 90676470DNAPopulus tremula x Populus tremuloides 76atggagatca aggaatcaga gagggtggtt atagctaaac cagtagcttc caggcctagt 60tactcaaatt ttacgtcttt ctcagagttg cttgcgggtg ccatcaatac cacaccctct 120aatgtttgtt ctggaacagc agttgctgcc attaaaccaa aaacagtgag gttcaggcca 180ctggtgaatc gtgctccagg tgcactggtt tcttcacagg ctggactctc tggaacagca 240gttagcaatt cgtttaataa agcttcaagt actgatagca aagccactat catatataaa 300ccacaggcaa agcttgtgtc aaaggcaact gtttctctac tggcgaatat gggaaacttc 360aatactaatt cacaacaaat gttacaacca gttgagactc gtcctcagct ttcaaaacaa 420gataaacaca atttctcatc ccagcttacc tcaaatcctc atcagaatat 47077302DNAPopulus tremula x Populus tremuloides 77atggaggagg ttgaggaggt acacaaggca gctattgaga gctgcaatag agttattggc 60cttttgtgtc aagaaaaaga tcaagtccag ggtaggaact taatggtgga aactagagag 120actgtgttta agtttaagag agttatttct cttttaagca ctggtttagg tcatggaaga 180gtaagaaaga tgaagaagct tagaccatct ttgccccaaa acatcttcct aaatagtcct 240aattgcaaaa caattttatc gccaaaacct ctccaaatgg tgcctcctaa ttttcttgaa 300ac 30278482DNAPopulus tremula x Populus tremuloides 78atggctggga ttgatgataa tgttgctata attggtgatt gggtacctcc tagtccaagt 60ccaagagctt ttttctcagc aatgttaggc gatgatatta actcaagaac aatctcagaa 120tctcctgggg agaaaagaaa tgaagggctc tatctgggac aaccagagca gatgacaata 180ggaaattctg agaaaaagga tggagcacaa actagtggtg ttcagttgac tgaattaggt 240acattttctg agcaaaaatc aaactttcgt ggaggtcttg ttgaaagaat ggcagccaga 300gctggattta atgcgccgcg gttgaataca gaaagtataa ggtctactga aagttctatg 360aaccctgaga ttaggtcgcc ttatttgaca ataccacctg gtttcagccc gacaaccttg 420ctggaatctc cagttttcct ttcaaatttg gcacagccat ctccaacaac tggaaaattt 480tc 48279538DNAPopulus tremula x Populus tremuloides 79atggaggggg ttgaagaagc taaccgggca gctgtggaga gctgccatag agttataact 60ttgctatccc aaccccaaga tcaggttcaa tataggaatt taatggtgga aactggagag 120gctgtgttta ggttcaagaa agtagtttcc cttttaaata ctggtttagg tcatgcaaga 180gttcgaaaac ttaagaagtt accgacccct ttatcccaaa gcatcctttt agacaaccca 240ctgagcagta caggccaccc atccaaaaca tcccagtttc tccagtccag tagttacctg 300gaaagccaat caattcaaga attgggctca attgctaaaa attgtctatc tctgggaacc 360ccatccctgg aattgagttc aaatgggaaa aaccctcttc agcttggaca acccacgcca 420gcagcgccct atcagttcct tcagcaacag caactgcata ggctacagct tcaacagcag 480cagcaaatga agcagcaagc tgagataatg tttagaaaaa acaatagtgg gatgagct 53880192DNAPopulus tremula x Populus tremuloides 80atggaattgt ccatggaatg ggagcacaag actctaatta gtgagctaag tcaagggaag 60gagctagcga aacagcttag caaccatctt aacccttcct catctctcga agcacgtcaa 120ttcctagtgg ataagatact ttcttcttat gagaaagcac tttcactgct aaattggggg 180gctttggtag at 19281394DNAPopulus tremula x Populus tremuloides 81atgatggaag aactttacgg tcttcaatcc accagccctg attactcttt gcaggttcca 60tcagagaaca tggttgctcc agtggctaat tactaccatc ctgcggggtt cacctcccca 120gctggggagc catcatttcc tgtttttgga tcggagcaga tgttttgtgg ttcctcagta 180tctgatgctg cttcaatggt ggccgagttg catcagaagc agcagcagca gagagggggt 240gttggtgtgg gtgatcatag taatagtaat aattcagagg aggtttcttg tgcaattagg 300gctaaaattg cttctcaccc gctttaccct aagttacttg aagcttatat tgattgccag 360aaggtgggag caccgccgga gatggcttat ttat

39482431DNAPopulus tremula x Populus tremuloides 82atggaaagag ataagttgtt tgtgagcgag ggagcgaaca cagcagcaac catttggaat 60tcttgcagtt ttggaatgga aatacaagcc aatgagctga gttgtggtcc agagaaactt 120gtcaattgct ttctcaatcc caattgggac aactcattgg atcagagcga tccctttgag 180tctgctttga gctccattgt atcctcacct gttgcatccg gtgccaatgc aaacgccaac 240gccgttccta atgctggcgt tggtggtgac ggttttatga ttagagaact cattggaaga 300ctaggaaaca tttgcaattc tggagacatt tcaccacaat cttttgttaa caataataac 360aatagcacta acacttcttg ctatagtacc cctttgaatt cccctccaaa gctgaatctt 420tcgatgatgg a 43183182DNAPopulus tremula x Populus tremuloides 83atgtctgttg gtgtaaacac agaaacccta gtgagagttg aggagataca aggaagagga 60agagggcttg tatccactca gcccttaaga ggtggccaaa tcgtcctcgt agattctcca 120atcctcctct attctgcact ccctttaacc aaacagcaac actcaacctt cctttactgt 180ga 18284463DNAPopulus tremula x Populus tremuloides 84atgtatcagc tggagagcgt tcctagttca agttcggtcc ataaaaactc attagttaat 60gatcagtatt tagattgtga tgatatgaca atggatccca tcaatggagg gaacaatctc 120aacaacaatc ctaatcttgc ctcaaagcaa agattgcgtt ggactcatga gcttcacgaa 180cgctttgttg atgctgtggc tcagcttggt gggccagatc gggctacgcc taaaggtgtt 240ctcagagtca tgggtgtgca aggcttaaca atataccatg ttaaaagcca tttacagaaa 300tatcgacttg caaaatacct tcctgactcc tcatctgatg ggaaaaaagc ggacaagaag 360gaaacagggg atatgatttc caatatggat ggttcatctg gcatgcaaat tacagaagca 420ctcaagctgc agatggaggt gcaaaagcga ctacatgagc aat 46385490DNAPopulus tremula x Populus tremuloides 85atgtctccac cactacttgg tgttgtggag gaggagggtc aaagtaatgt cactctactg 60gcttctccgg cctctgcaga aagtgcatgc ctgaatggtt tggaattgaa agagcgtaac 120tacatgggtt tgtccgattg ttcttccgtg gacagctcgg cagtctctgc agcatctgat 180gaaagaaagg ctagtttaaa tctgaaggct actgaattgc ggcttgggct tcctgggtct 240cagtctccgg aaaggaatca cgagctttcc ctgttgagct cggcattact tgatgagaag 300cccttcttcc ctttgcatcc ctcaaatgat ggtcactact caacacagaa aaatgttgtt 360tcgggtaaca agagagtgtt ctctgatgcc atggatgagt tttcagagag caagtttctg 420tcaaattcgg aggtaaatgc aatgctctca cccaggccct caccgaacat gggattgaaa 480cctggcatgt 49086469DNAPopulus tremula x Populus tremuloides 86cggcagcaaa gagaaaagat gaaatgaaaa cgacacagcc tgtttcgata attctcatta 60ccagcacaag actgacatca tgaagaagcc tccgtaactg aaacagcttt gtttttgttt 120ttgattgtca tgttatttag cttttccgga gccctcatac tcttctgata tctgggccag 180agaaagcatc aagttcgata tcaaatcctt ccatggctga gatcactgct tgactctagt 240agtaggtgcc tgtgaatcag gttcaaaggc tgctaggttt gtgctcttca gagggatctc 300atggttatgt tttccttcat atgttgtaat aaaagctctt ggatcatctg atactctttc 360cacatgcttg cgaacattgc atttgatact ggtacatcta tagtaactcc tgggatatgg 420attccccttc acaatcttct gcccatattt tctccatcga aaaccatcc 46987440DNAPopulus tremula x Populus tremuloides 87gtttcatgta tgcgcagact gtgatgagat caacctggaa tggttatgct caccttcata 60ggtgacaatt agcattgaag ggtcttccag gcatctctca acgtgcttcc ttgccgggca 120acccctcaga ctgctacatt tatagtatcc cctaggatgc ggagagccct taattggctt 180ttgtccatac ttcctccatg aatactcatc aggaggaata tctgccacct tgttgctaat 240tgcaggaact ttgatagatc tcttcaccct cattttcctc ctctttgaac aatggcattt 300accactgcta gaacatttgg cattcccatc ttctcctcta ccagaacacc tcctccttgt 360ttgttgtgag atgtgatcag atgagtgagg cataccaatc aaatggaaag agtctccatc 420aaaattagac acagcaccat 44088514DNAPopulus tremula x Populus tremuloides 88attttacatc tgaggcagcc tactcattaa atgctgatac actgctgggt tgttggagga 60caggctcata ttagcttcag tcactggctc cacctttgct tctcctttcg gcatcatcaa 120ccccatttca ttcactgcac gttgctgtgc taggtatgga tgcatgggca taactggcat 180ccttggttgc cctggaccta acccagccat tcccatattg gcaaacccag gttgattcat 240tccaaatgag aagccagccg gaggccccag ctgctgcctg aaggacccaa atgcaggagg 300cctctcaaat ctaccagtca tgctgttgtg aacttgggac gcctcaggtc tatgaacttg 360ggtttgaacg gcgacgccag cttggggtgg tgtggcatta gaggtgccag agttgacatg 420gttgctattg cgagcagcag ggacatcatg attgtgcttc ccctcgtacg tagtgatcac 480tgacttaagg tcatgcgatg ccctctccac atgc 51489454DNAPopulus tremula x Populus tremuloides 89gggtggttac gtgtttgtgg attgtgctgg aatccttggg tggttatgtt caccctcata 60ggtaacaata agcatggacg gatcttccaa gcacctctcc acatgcttcc ttgcaggaca 120acctctcata ctgctacatt tgtaatatcc cctaggatga ggagaaccct tgattggctt 180ttgcccatac tttctccatg agtaatcatc aggagggata tcagcaagct tgttgctaat 240agcaggaacc ttgatcgacc tcttcaccct atgtttcctc ttctttgagc aatgacatct 300aacgctgctt ccacatttca tactcccatc ttctcccctt ccggaacatt tcctcttgtg 360ttgctgtgaa ctctgatctg aggagcgagc cgcccccgtt aaatggaatg cagttccttc 420caaattagcc acattaccgt caatactcaa ggac 45490270DNAPopulus tremula x Populus tremuloides 90ttaagcaaag aaatctgggt tgtcaaatgg gaaagtggta tcaaaatcta cattatcgat 60tgagatatca aaatccccga ttggtgaatt ggttaccgaa gttggggctg atattatctc 120cgtggtgagt tcagactccg gggtctgcac atttgggtaa cctattccaa agctgttcat 180atggcatggt gacattgaga agtagttgga ttcagatgtt gccggagata taaatgtcgg 240agaaaagcta cccaagaagt tgttttccat 27091465DNAPopulus tremula x Populus tremuloides 91catcagcatg aggccctgtc caagcatgta caaaaaaagg cgtagtgaga gctaagaggg 60aacaccccac ctcagtcgtt catgaaaaag ggaccataaa gactatccac aacagcaaat 120tgcatgttct ctgatggttt ccagcgacgt ttcctttgat tgatgaacca gttgtttatc 180tgtttctgat ccaggccagt tgattcagcc aacgcaacct tgtcagcttc cgttgggtat 240ggccatttgt tatgaacgtt ccaccagttt agcaggattt gccttgcttc ctttggtagt 300tttcctttct tcttctgttt tgaaaacgca tgctttaggg tacttatgta accactatat 360ttacgtagta gtttgtcctt gagctctcga tcctcgttta tacgggtaga atcttgcaag 420tctgcctctc ctccactggc atcgtcatca gatgaaccgg cagct 46592389DNAPopulus tremula x Populus tremuloides 92gggatcaggg actgtttatt ggttaattac agctcaattt tcatgtgagt tgagggcact 60gagccttgaa agctctcctg atgattctga ccatatccca tctgcacaac actttgaagg 120tcgtcttccc acatggctga ggcctgaaga acagcaggat caccaaaacc atcaaggggt 180ggcagatgca tgctcgggtt tcgccgcaac gcagcgttta atgggttcat ggagaactgg 240gtttctgcat tgcttggcat gccattttgc agggccagcc cttgctggga ttggtatcca 300taagggaatg ccggcgtgga ggcatctgat ggataaagac tatgaggcat ggatccacgg 360gattggaaaa tatccttgga caacagagt 38993201DNAPopulus tremula x Populus tremuloides 93cagcatcaac aaacctcatc attcttgata acaccacagg cattgcattc caataaattt 60gatggcttag catcagaagg aggaagagga gctagtgtac cccaacaatt atttcgatta 120cacatgtatc tcaaaaagaa ataggcatga ggaaagtcag cgctgttatc attgtcgatt 180tcttcaccat tagattcggc c 20194483DNAPopulus tremula x Populus tremuloides 94ctaaacatca ctgcctgaaa ctttctccat ttgatcttcg attcccatcg ataacccaga 60tgaaggatca aactgctctc tacccaggaa ggcagagtgt ggtcgtggat aggaatttaa 120gcttgactcg agtatctggt gtgtaagaac catctcttgt ttactgtatg ttacacccat 180gcttacccgt tgcttcttca tcgatctctc acctctaggg ctctctgctg gggagccagc 240attacaacgt gaatctggtg tcagtggctc aggctgagaa gagaatgatt catcaattga 300aaggctcttt gctggggcac gttccttggc agccttgtct tggaggggtg attcagaagt 360tggggcaggg gttgctgggt ctgtcttgtc agattctggg cagttatcac ctgatactgg 420ggcagcgacc cccgagccag gcacatcttc aagaactcca ctcaatcgtt gttgctcttc 480aat 48395374DNAPopulus tremula x Populus tremuloides 95ctaattcctg tttttgcatt tctccatggc ccttggtgct aggccaatgg catcagagct 60cttcatgatc ctcagcctct tgcatgtttc aataaacatc tcccagggaa catcgccaac 120aagcatccag tctccgtctt tatcctcata agtgagaaca tattctgagc catgcagcag 180atccttcagc ttgctctcgc tcagcatctc ccttcctgga gctccatggg atccatattg 240gcctatggtg aaacagctga acatcttctc gagggcagaa gacaattcct gatatgcaga 300gtagtttctc aaatccactt ttctaagata aggagcacca tccatgctga ctttgataaa 360taaagcacct ggcc 3749611135DNAArtificialBinary destinatin vector pK2GW7 96ctcccatatg gtcgactaga gccaagctga tctcctttgc cccggagatc accatggacg 60actttctcta tctctacgat ctaggaagaa agttcgacgg agaaggtgac gataccatgt 120tcaccaccga taatgagaag attagcctct tcaatttcag aaagaatgct gacccacaga 180tggttagaga ggcctacgcg gcaggtctca tcaagacgat ctacccgagt aataatctcc 240aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact 300gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga 360cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc tctaagaaag 420tagttcctac tgaatcaaag gccatggagt caaaaattca gatcgaggat ctaacagaac 480tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga 540aaatcttcgt caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata 600cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc 660tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag 720gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg 780ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg 840ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg 900acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt 960tggagaggac tccggtattt ttacaacaat accacaacaa aacaaacaac aaacaacatt 1020acaatttact attctagtcg acctgcaggc ggccgcacta gtgatatcac aagtttgtac 1080aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg 1140cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg 1200cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga 1260gttaggatcc ggcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg 1320gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt 1380cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga 1440ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga 1500tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata 1560gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga 1620gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt 1680acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag 1740ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct 1800tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc 1860tggcgattca ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg 1920aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga tccggcttac 1980taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata 2040ctgatatgta tacccgaagt atgtcaaaaa gaggtgtgct atgaagcagc gtattacagt 2100gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt 2160ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg 2220gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct 2280gacgagaaca gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg 2340ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt 2400gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt 2460ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt 2520ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc 2580cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc 2640aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat 2700gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct 2760tgtacaaagt ggtgatatcc cgcggccatg ctagagtccg caaaaatcac cagtctctct 2820ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag 2880ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat 2940gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc 3000agtgacctgc aggcatgcga cgtcgggccc aagcttagct tgagcttgga tcagattgtc 3060gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta 3120agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg 3180ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga 3240tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg 3300aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct 3360ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga 3420gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca 3480ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc 3540tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg 3600accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca 3660cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc 3720tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg 3780ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg 3840ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg 3900cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc 3960ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca 4020ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg 4080cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga 4140cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt 4200cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga 4260tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct 4320aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat 4380gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac 4440cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc 4500gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg 4560gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc 4620gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg 4680gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc 4740ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc 4800acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc 4860ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc 4920acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa 4980cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc 5040atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc 5100gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca 5160tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg 5220tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt 5280aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa 5340aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt 5400tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag 5460cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct 5520gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga 5580ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc 5640ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca 5700accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat 5760ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta 5820cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg 5880ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg 5940ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa 6000gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg 6060gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt 6120gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg 6180gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg 6240caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg 6300ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt 6360caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt 6420gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca 6480ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg 6540ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa 6600aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg 6660gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac 6720tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac 6780tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca 6840aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat 6900cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg 6960acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc 7020gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 7080gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 7140ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 7200ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 7260cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc 7320gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7380gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7440ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7500cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7560ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7620taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7680ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7740ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7800aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7860tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7920agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7980ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8040tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8100tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt 8160gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg 8220ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg cgaagcggcg 8280tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg

atctcgcctt 8340tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt 8400gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct 8460ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc 8520aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt 8580tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa 8640agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca 8700agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc 8760gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt 8820cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg 8880aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca 8940ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt 9000acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg 9060atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag 9120ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg 9180cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca 9240taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt 9300ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag 9360gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt 9420acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc 9480cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc 9540gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg 9600ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac 9660gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt 9720gaattatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg cgcgataatt 9780tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga 9840ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat 9900gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca 9960atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag ctagagtccg 10020aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg 10080aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct 10140cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc 10200ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg 10260catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga 10320acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac 10380cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc 10440aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct 10500cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc 10560agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg 10620ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg 10680tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc 10740agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag 10800aacctgcgtg caatccatct tgttcaatca tgcctcgatc gagttgagag tgaatatgag 10860actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa 10920tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg 10980tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg 11040gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt 11100gactccctta attctcatgt atgataattc gagct 1113597564DNAPopulus Trichoocarpa 97atgcaaccag gcttggatga gatcacaatg acggcgtgga agaatctaaa gcaacggctc 60tcattcaagg gcctgggtag ttgctgcggg agcacaagct ggagttccag aagtgccacc 120ccaaccatgc cctttatcga tatagagcaa gaagaagagg aagagcccat catgcaaaac 180caagctcaaa gaggaggagg agctgcagca gcagcagcag cgccaggtgc tgggatgaat 240ctggcaatgg cattagctgc tgagcgcaat ttaggggatt caaatgtcaa gacattgatg 300agtttgatcg aagaaacgga cggtgttgat tggaggaaga agaataacag taatgataaa 360agtaggaggg acaaggaaca ggaacagaag caggaagaag agaaggattg ggtatgctgc 420gtttgcatgg agagaaataa aggcgcagct tttattccat gtggacacac cttttgtagg 480gtttgttcaa gagaaatgtg ggttaatcga gggtgctgtc ctatctgcaa ccgttccatt 540ctcgacatcc ttgatatctt ctag 56498645DNAPopulus Trichocarpa 98atggcgagag gaaaggttca gctgaaaagg atagagaatg caactagcag gcaagtgacc 60ttctccaaga gaaaaaatgg gttattgaag aaagcttatg agctatcaat tctgtgcgat 120gctgaagttg cagtgatcat cttttcacag aaaggaacac tctttaagtt tgcaagcatt 180gatcagatac aaaagacgat tgatcggtac cgtaaaaatg caaagcaatt gcacactgac 240aggattgatg tggaacaatc taaggagcaa ttaagacaag aatcagcaaa catggccaag 300aagattgaga taatcgagat tttgcaacga aagcttttag ggcaagattt agattcatgt 360tctcccgaag agctccatga cattgacaat cagcttgaga tcagtttaag caatatcagg 420gctagaaaga ctcagttatt caaggagcag atagaacagc tgcaagcaaa ggaaagattg 480ttgttaatgg agaatgcaag gttaactaaa cagtgtgatg cacagccatt gcagcaatca 540actcaatcga accaagtggt gtcatacttg acctcatgta gcaagagttc agatatcgtg 600gagactgatc tgtacattgg actgccacac atgcgctgct tgtag 645991755DNAPopulus trichocarpa 99atggcatata tgtgtgcaga tagcggaaat ctaatggcaa tagcccaaca agttattaag 60caaaaacaac aacaagaaca acagcagcaa caaagccacc accaccacca taaccaacag 120caacaatttc ttggcctaaa cccattttct ttaaatcctt ggccgactaa tataatgtct 180gctaacccaa acttgggtta cgggctctcg ggtccggctg ctttctctga cccttttcag 240agtggacaag aaacaggtga cccacctgtg tttagtttct cgaacatgga gcagcaccac 300tcaagcgggt ttcggcttcc ttattttact ggagctggtg gtgagtttga ctcggatgag 360tggatggaca gtttaatgaa cggtggagat tcaacggata gttctaatct tccttctggt 420tgtgacgcgt ggcaaaacag tactgatttt gggatgtaca cttctgatcc gtttaatact 480tgccccagtc gacttactgt tggctgctct ccaccgtctg atcttaacgg ggttctctct 540aattcgctct gggtcgcccc atctcctcct caagaaatca agcccacaac gtcacccccg 600ccactgcttc cgccaacagt aaaaaatgaa accgtcggag ggtcagagga aattgtacag 660ttatcttcct cgccggtttt gaaagcgctt gttgagtgcg ctcaacttgt cgagtccaaa 720gctgatcaag ccgtgaaatc gttggttaag tgcaaggact tggtgagtga aaacggtgat 780ccagttgagc gagctggttt ctacttcgcg gaaggattat gccgaagagt tgctgtagga 840gagcttgatg tcttgaaaaa ttttgatcag acaagtgaag agttcactct gtcctataaa 900gctttgaatg atgcctgtcc atattcaaag tttgctcatt taacagcaaa ccaagcaatt 960cttgaggcaa ctgagaaagc aagcaagatt cacatagttg attttggtat tgttcatgga 1020gttcaatggg ctgctctttt acaagcttta gctacacgtt cagctggaaa acctgttaga 1080attcggatct caggtatacc tgctccagtt cttggtaaga atccagctgc ctctctttta 1140gctactggta ataggcttct tgattatgcc aagcttcttg gtttgaattt cgagtttgag 1200cctattctga ctccaattca ggagttaaat gaatcttgtt ttcgagccga gccggatgag 1260gttttggctg ttaatttcat gcttcaattg tataatttat tggatgagtc tccagttgct 1320gtagaaactg ctttaaagat ggctaaatcg ttgaacccga taattgtcac tcttggtgaa 1380tatgaagcta gtttgaaccg ggttgggtac ttgactcggt ttaagaatgc tttgagatac 1440tacactgctg tttttgagtc tcttgagcct aacatgagta gagactcacc agagaggctt 1500caagttgaga gattgttatt gggtcgagaa tttctagtgt tggaaaggat ggaggataaa 1560gatcaatggg gagttttaat ggaaagttca ggttttgaat cggtttcgct tagccattat 1620gcaatgagtc aggctaagat actcttatgg tattgcaatt acagtgattt gtattctctt 1680gatgattctc agcctgggtt cttaacttta gcttggaatg aggtgccact actcacagtt 1740tcgtcatggc gataa 1755100729DNAPopulus trichocarpa 100atggggagag gtagggttga gttgaagaga attgagaaca agatcaacag gcaagtaaca 60tttgcaaaga gaaggaatgg acttttgaag aaagcctatg agctttccgt tctttgtgat 120gcagaggttg ctctcatcat cttctccaat agaggaaagc tgtacgagtt ttgcagtagt 180tcaagcatgc tcaaaactct tgagaggtac cagaagtgca attatggagc accagagcca 240aatgtgtctg caagggaggc cctggaactg agtagtcagc aggaatatct gaagcttaaa 300gcacgttatg aagccctgca aagaacccaa aggaatcttt tgggagaaga acttggccct 360ctaagcagca aagagctaga atcacttgaa aggcagcttg atatgtcatt gaagcagatc 420agatcaacaa ggacccaata catgctggat cagctccatg acctacagca taaggagcac 480atgctgactg cagctaataa gtccctgaaa gaaaggttga tggaaggcta ccaattaaat 540tcactccagt tgaatccaag tgcagaagat gtggagtacg ctagacaaca agcccaaccc 600cagggtgatg ggttttttca tgctttggag tgtgaaccta cactacaaat tgggtatcag 660ccagaaaata taacaatggt cactgctggc ccaagtatga ctacttacat gccaggttgg 720ttagcatga 7291011758DNAPopulus trichocarpa 101atgtctttag ttggacctgc agaactttct gcaacaccat acggaaatca taagctgtac 60tcactgaagg ggagcaatga caactctggc ttgtctgccc aaatattctg ccctgataaa 120cgtcagaaca tgtatatgac cgattcttac tccagtgaga gttatgagaa gtacttcctt 180gattccccaa cagaagagct aatacaacca tcaagttctg gcatttcagg gaattcagct 240ccacctcaag gcacatcttc ctaccagcta agaaagaatt taggtccatc catgtcccct 300caagatgatc catacgacgc ttgtttcaca ttgacgacac cctgtgatgg ctatcaattc 360aattccgagt cagattactt ggacatcgag agcccagatc cactaaacta tgatgaatat 420aagatgaaat taaagtttca ggaacttgag agagcgcttc taaatgataa tgatgaggat 480ggcatgtttg gaaatagtca gagcatggaa atggatggag agtggtctga tccaatccag 540aatgggatgc tccatgattc tcccaaggag tcatcatctt ctgattctag cctaagtagc 600atcagcagta ataaagaagt atcacagtta tctcctcgaa ctcccaggcg attgcttttt 660gaatgtgcta atgcaatctc agagggaaac attgagaaag catcaacttt aataaacgag 720ctccgtcaat tggtgtcaat tcaaggagat cctccgcaga ggattgcagc ctacatggtg 780gaaggtcttg cagctcgtat ggctgaatct ggaaaatatc tctataaagc tctgaaatgc 840aaggaacctc cttcttctga taggcttgca gctatgcaga tcctttttga gatttgccct 900tgttttaaat ttggatttat ggcagcaaat ggtgcaatga tcgaggcatt taaaggtgaa 960aggagagttc atataataga ttttgacata aaccaaggga gtcaatacat aacactgata 1020caaacactcg ccaaccatca aggtaagcta ccacacttga ggttaacagg ggtcgatgac 1080cctgagtcag ttcaacgacc tgttggtggc ctgaggatca ttgggcaaag gcttgaaaag 1140ctagcagaag catataaggt ctcgttcgag tttcatgcag tggcctccaa gacttcactt 1200gtcaatccat caatgctgaa ctgcaagcct ggggaagcac ttatagttaa ttttgctttc 1260caactccacc acatgcctga tgagagtgtt tcaacggtaa acgagagaga ccagcttctt 1320cggatggcta agagcttgaa tccaaaacta gtaactgttg ttgagcaaga tgtgaatact 1380aacactgccc cttttttccc gagattcacc gaagcttaca actattactc tgctgtgttt 1440gactctcttg atgcaactct cccacgggag agccaggata gactgaatgt tgagaaacag 1500tgcctggcaa gggacatagt gaacatagtc gcatgtgagg gagaggaaag gattgagcgg 1560tatgaagttg cagggaaatg gagagcaagg atgatgatgg ctggcttcac ttcatgttca 1620atcactccta atgtggttga tatgatccgg aaactcatca aggagtactg cgatagatac 1680atgttgaagc aggaagtagg tgcgcttcat tttgggtggg aggacaaaag tttgattgtt 1740gcttcagcat ggaagtaa 17581021635DNAPopulus trichocarpa 102atgaagagag atcatcaaga aaccattggt ggtggtattg gaaacagagc tgaatcttct 60tcttcatcaa tggaaactgg gaaaggaaaa tcatgggttg aagatgatca agatgcgggt 120ggcatggatg aattacttgc tgttttgggt tacaaaatta agtcttcaga tatggctgat 180gtagctcaaa agcttgaaca actagagatg gttttgggtt ctgaagatgg aatttctcat 240cttgcttctg atactgttca ctataaccct tcagatctct ctggttgggt tcaaaattca 300ctccttggac aaccttccac tatcaccccc ttagatttcc ccagtaattc tcaatctaaa 360gtattcgctg acgactctga atatgatctc agagcaatcc ctggagttgc tgcctatcca 420caacaagaac tcgataagac aatggcagtt tcaggtactt tgtcagagcc cacaagacct 480gtagtcctgg ttgactcaca ggaaactgga gtccgtcttg tccacacact tttggcttgt 540gcagaggcaa ttcagcaaga aaatcttaaa cttgctgatg cccttgttaa gcatatagga 600ctacttgcag catctcaaac tggggctatg agaaaagttg caacttactt cgctgaagct 660ttagctcgtc gaatttacaa gattttccct caagattact gtctcgattc ttcatgttca 720gatactttag agatgcattt ctacgaaact tgcccttatc tcaagttcgc acatttcact 780gccaatcaag ccattcttga agctttcgcg aatgcaagtc gagtccacgt tatcgatttt 840ggtttaaaac agggaatgca atggccggcg ttaatgcaag ctcttgcatt aaggcctggt 900ggtccgccag cgtttcggtt gacggggatc gggccgccgc agcctgataa cacagatgct 960ttgcagcaag tggggtggaa gctggcacaa ttggctcaaa ctattggagt agaattcgag 1020tttcgcggat tcgtagcaaa ctctctggcg gatcttgatg cggagatgct tggcctcctc 1080cctccagagg tggaggcagt ggctgttaac tcggtttttg agctgcatcg cttgttgggt 1140cggccgggtg gcatcgacaa ggttctcgag tctatcaagg ccatgagacc taaaattgtg 1200acgattgttg aacaagaagc aaaccacaac ggtccggttt ttctagaccg gttcacagag 1260gccttacatt attactcgag tttgtttgac tctctcgaag ggtccggttt gactccaccg 1320agtcaggacc tggttatgtc cgagctgtac ttagggaggc atatttgtaa tgtggtggca 1380tgcgaagggg ctgaccgagt tgagcgacac gagacgttgg ctcagtggag aactcggttt 1440gattcggctg ggtttgaccc ggttcatctt gggtcgaatg cctttaaaca agctagtatg 1500ttgttggccc tctttgccgg tggtgacggg tatagggtgg aggagaataa tggctgtctc 1560atgcttgggt ggcatacaag gccactaatt gccacctcgg cgtggcagct cgccgctggt 1620gattcacaac aatga 16351031059DNAPopulus trichocarpa 103atggaaaaca attgtgtgta tagtgaggaa gatgagcaga tggaattgcc tccgggattt 60agattccatc caactgatga agagctcata actcactacc tgtctcagaa ggttctggac 120aactactttt gtgctagagc tattggtgag gtcgatttaa acaagtgtga gccatgggat 180ttgccttgga gggctaaaat gggtgaaaag gaatggtatt tcttctgtgt tatagaccgg 240aagtatccca ccggtttaag gacgaatagg gctactgatg ctggttattg gaaagccaca 300ggcaaagaca aggaaattta cagggctaaa acacttgttg gcatgaagaa gaccttggtt 360ttctacaaag ggagagcccc caaaggagaa aaaaccaact gggttatgca tgagtataga 420ttagagggga aaaaccccgt ctataatccc cctaaaacag caaagaatga ttgggtcatc 480tgtagaattt ttgagaagag ttgtggagga aagaaaacac atatttcagg gttggtgagg 540ttaagctcgt atggaaatga attaaagcca acaatattgc ctccattgat ggattcttct 600caacacaata acgacaagag gactaacata ggtgataagt ctcacgtgac ctgcttctcc 660aatccaacgg aggaccagaa accccatgaa accattgctg attgctttaa catttctctt 720agagctcctt tgtcttcttc aaacatgtcc ccttcctcag ttctgttttc aaaaccctca 780cctccaaact ctttctattc ctctcatatt ttaccaaaca tagcaaattt tcaataccca 840gattctgtta tgatgccaga acactccatg ttgaggatat tgcttgaaaa ccaaggaccc 900ggcatgaacc taaactcaaa acgggagctc tcagaggaca ctggcctcag cactgatatg 960tcttcagtgg taaccaacca tgaattggtt catgggtcct ttgaggatcc atcaagttct 1020gccggaccag ttgatcttga ttacctttgg aattactga 10591042463DNAPopulus trichocarpa 104atgagttttg ggggtttcct tgaaaacact agtcctggtg gtggtggcgc aagaattgtg 60gctgatatac tttataacaa taacaacaac atgcccactg gtgcaatagc tcagactcgc 120cttgtctctc cttctatcac taaatccatg ttcaactctc ctggactctc tctagccctt 180caacagccaa acatagatgg tcaaggagat ataactagaa tggctgagaa ctttgagaca 240agtgtgggta ggagaagcag agaagaggaa catgagagca gatctggtag tgataacatg 300gatggtgcgt ctggtgacga tcaagatgca gctgataatc ctccaagaaa aaagagatac 360caccgacaca ctccacaaca aatccaagaa cttgaagctt tgtttaagga atgtcctcat 420cctgacgaga aacaaagatt ggagcttagt agaaggctgt gcttggagac taggcaagtc 480aagttctggt ttcaaaatcg tagaacccaa atgaagactc aactggagcg ccatgagaac 540tcattactca ggcaagataa cgacaagctt cgagcagaaa acatgtccat aagagatgcc 600atgagaaatc catcgtgctc aaactgtggt ggtcctgcga taattggtga tatgtcactt 660gaagaacagc atttgaggat tgagaatgct agattaaaag atgaactaga tcgagtttgt 720gcacttgcgg gcaagttctt gggtcgcccc atatcttcgt tagcttcttc acttagccct 780ccgacgaatt caagcctgga acttgcagtt ggtagtaatg gttttgctgg tttaagcacc 840attgctacaa cattgccttt gggacctcat tttgaaggtg ggatttctgg tgctttgtct 900atggtaactc aaactagact agcaacagct ggtgttactg gtattgatag atcagtagag 960agatccatgt tcttggagtt ggctttagct gccatggatg aattggtgaa aatggttcag 1020acggatgagc ctctttggat cgggagcttt gagggtggta gagaaatatt gaaccatgag 1080gggtacttga gaaccttcac tccttgcatt ggaatgaagc ctagtggctt tgttagtgag 1140gcttctagag agactggtat ggtaatcata aacagtttgg ccttagttga gacactgatg 1200gattcgaacc gatgggcaga aatgtttcct tgtatgattg caaggacctc taccactgat 1260gtgatagcca gtggaatggg gggaactaga aatggttcac ttcagttgat gcaagccgag 1320ctccatgttt tatccccatt ggttccggtg cgtgaggtca attttctccg attttgcaag 1380cagcacgcag agggggtttg ggctgttgtt gatgtatcca ttgataccat ccgagatact 1440tccggtgcac caccgacatt tgtgaactgc aggaggcttc cttctggttg tgtggtgcaa 1500gatatgccca atgggtactc caaggttaca tgggttgagc atgcacaata tgatgaaagg 1560caaatacacc agctctatcg gccggtgata agctccggca tgggcttcgg tgcccaacga 1620tggatagcta cccttcaacg tcaatgcgag tgtttggcca tcctcttgtc ctccaatgta 1680cctagtagag accacacagc gataactaca agtggtcgcc gaagcatgtt gaagctggcg 1740caaagaatga ccgataactt ctgtgctggg gtttgtgcct ccacagtgca caaatggaac 1800aagctgaatg ccggaaatgt tgatgaagat gttagggtta tgacccgaaa gagtgtcgat 1860gatcctggtg agccaccagg catagttttg agcgctgcga cctctgtttg gctacctgtt 1920tctccacaaa ggctctttga tttcctacgc aatgaacgac tcagaagcga gtgggacata 1980ctctccaacg gtggaccaat gcaggaaatg gcccacattg ctaaaggcca ggatcacggc 2040aactgtgtct ctctcctacg tgctagcgcc atgaatgcta accagagtag catgctaata 2100ctgcaagaga catgcataga tgcagcaggc tctcttgtag tgtacgctcc cgttgacact 2160ccggccatgc acgtagtgat gaacggtggt gattcggctt acgtggcgct tcttccgtca 2220gggtttgcta tcgtaccgga tgggcctggt tcacgtgacc ccccatcgac taatggcggc 2280ccgactgcta ataatgtcgg tggccaagag agggtgagtg ggtccctttt gacggtggcc 2340ttccaaatat tggtgaatag tctacctacg gcgaagctta cagtagaatc tgtggagaca 2400gtcaacaacc ttatttcatg cactgtccaa aagatcaagg ctgctctcca atgtgaaagc 2460tga 2463105633DNAPopulus trichocarpa 105atggcaccca agaagcttaa tggtaatgac aatggttctt tgaagaaagc aagtggtgat 60catgacaaga aggaaatcca ttatagggga gtgaggaaga ggccatgggg gaggtatgct 120gctgagataa gggatcccgg gaagaaaagc cgggtttggt tgggcacgtt tgacacggca 180gtggaggctg cgagggccta tgataaggcg gcgcgtgagt atcgtggtgc taaggcgaag 240accaactttc caatagcgga gaaggtggtt gattatgacg atgagaagca gagctctagc 300cagagcagca ccgttgagtc gtcaagctcc ccggtggttt ctgcggtggc gcgtgatgta 360actcgccagg ttggtggggt tgtggggatg gggaggtttc cctttgtgtt ccagcagcag 420ccgccgcatg ttaacgctgt tggtcctgtc tggtttcttg atagtactgt taagcctgag 480tttgtggctc agcgtttccc tgtccggtat gacccggtgg gtcttgaggg cggggcccat 540agtgactcgg attcatcatc tgtgattgat tttaagccaa ggagttcaat tcttcatctt 600gatcttaacc tgcctccacc agctgatgct tga 633106732DNAPopulus trichocarpa 106atgtgcacta gaggacattg gaggcctgct gaggatgaga aacttaagga attggttgaa 60aagtatggtc ctcataattg gaacgccatt gccgaaaagc ttcaaggaag atcagggaag 120agttgtaggt tgagatggtt taatcagctg gatccaagaa tcaatagaag cccgtttaca 180gaagaggaag aagaaagact acttgcttcc cacaggattc atgggaatag atgggcaatt 240attgcaagat ttttccctgg tcgcaccgat aatgcagtga agaatcattg gcatgtcatc 300atggcaagaa gatatagaga gaggtctaga cttcatgcaa aaagggctgc

tcaaactttg 360gtaaatgata acaaattatc ctcaaaacaa gatcacatgc acatggattg tgagacgagg 420aatttttctt cattttccaa gaaatattgt gaaaaatatg gccaatatcc tatggttact 480cacagctact taccggcctt ttgcaaagag ttctacaatg aagatccaag tcattgtgaa 540gatcaaagtc ggccgattga gttttatgat tttctccaag taaacactga ctccaataaa 600agtgaagtga tagacaatgc aagaagagat gatgaggagg tagatcagca ggaagccttg 660gaaaataatc agagcaaggc tgatgttcca ttcattgatt ttttctctgt taatggcaaa 720tcctcatcat aa 732107988DNAPopulus trichocarpa 107atgggcagat ctccttgttg tgagaaagaa cacaccaaca aaggtgcctg gactaaagaa 60gaagatgaac gtctcattaa ttatatcaaa tctcatggtg aaggttgttg gaggtctctc 120cctaaggcag ctggtttgct tagatgtggc aaaagttgta gactgaggtg gataaactac 180ctaaggcctg atctcaagag aggaaacttc agtgacgaag aagatgaact catcatcaac 240cttcacagct tacttggaaa caagtggtct ctcattgctg ctaggctgcc aggaagaact 300gacaatgaga taaagaatta ttggaatact catatcaaga gaaagctctt tagccgtgga 360gttgatcctc aaactcaccg tccactcaat tccaccacca cctcctccac cacttccacc 420accacaaaca gcaccaacaa taaaaatagc aacatgggca ccaaaaggat tactaacttt 480aaacttgaag aacaaaacta tttatttgtt caagcccagc cagagtttat gatgagcaac 540atagtcaaga aggctagcga ttcaagtatt atcaaggtag gtggcagcag tgactctgct 600gaagattcca atagtagcag tggcgtgaca gcagaattag aagtgcatcc aaatcataaa 660ctcaatcttg agctctctat tggtcttcca tgtcaatctc aactttcttc tgtcaatgat 720cttaatgatt caaagcaagc aaaccaacag catcaagaac aagtagtcac atatcaattg 780tttgggaccc ctgctactcc cacctccagt gcccctgctg ttgttcatag aacagcatgt 840ttgtgttctt acaatcgagg gttcaagaac agtcaagcat gtagttgttg taatgccgtg 900gaaaaatttg taacagctga tagtctttat agattttaca gacctttgga tgcttgatga 960tgaccgggga tcagtttgat aaggctag 988108708DNAPopulus trichocarpa 108atggaggttt atccataccc atctcgtttt tccatgtctt cattgtgttc ttttgggaat 60tttgttgata aggttaaaga agtttgtaac ttcgttgttt cagctattat tggcaacata 120ttctctgcga tcttcacctt tttctttgca ttagtgggca ctttgttagg agccatgact 180ggggcattga taggccaaga aactgaaagt gggtttgttc gaggggctgc agttggagcc 240atatcagggg ctgttttctc aattgaagta ttcgagtcat ctcttgttct ttggcaatca 300gatgaatctg ggataggctg tgtcctttac ttgattgatg ttatcgcaag ccttcttagt 360ggacgacttg ttcgtgagcg cattggtcct gctatgttaa gtgcagtaca aagtcagatg 420ggtgctgtgg aaacaaattt tgaggagatc ccaaacatct ttgacactgg tggttccaag 480ggattacctg gagattctct tgagaagatc ccgaagatca gaatcacaag caataacaat 540gtagatgaat caggagagaa agtctcttgt tcagtttgcc ttcaggactt tcagctggga 600gagacggtta gaagcttgcc tcattgtcat cacatgtttc acctaccttg catagataag 660tggctactta ggcatgcatc ctgccctctg tgtagaaggg atctgtga 708109882DNAPopulus trichocarpa 109atgagaatat ctagagcaag atggttcacc ttcttgagga gagtattcca ctaccagaat 60ggctcaagct ctaatcttgg gtctaatcct ttcaattcta gctcttggat gatgctggag 120ttcgtagctt tgctccttca aatatgcatt accacgttca ccctggctat ttcgaaggca 180gagaaccctg tttggcctgt gagaatatgg attattggtt ataatatagg ttgtgtcctt 240agtctgctgc tgctctatgg tcgttaccgg caacttaacg caactcaagg cgatggtttt 300ggcctacccg atttggagca acaggggggc agtgaggaat ccagtgtatg caggtactca 360catttgatga acaagtgccg gacctcactg gaactcttct ttgcgatatg gtttgtgatg 420ggtaatgttt gggtcttcga ttctcgtttt ggatcttact tccgtgctcc aaaactccat 480gtgctctgca tctctctgct tgcctggaat gctctcagtt actcgtttcc atttctgttg 540tttctactgc tatgctgttg tgtgccgctc attagcactg tcattggcta caacatgagc 600atggggtctg ccgagagagg ggcgtccgat gaccaaatat ccagactacc aagccggagg 660tacaaagctg tggacaccga ttcggagttt cgcaacagtg ttgattgtga ttcaaccgtt 720gcaagtgaag atctggaatg ctgtatatgc ctagccaagt ataaagacat agaagaagtt 780aggcaattgc catgctccca tatgtttcac ctcaagtgtg tagatcaatg gcttcgaatt 840atatcctgct gccctctctg taaacaagaa ctggaaaaat ag 882110966DNAPopulus trichocarpa 110atggatccca agggctctaa ctcaaaaaac ccacatgagc tacccacttt ctttaccctt 60acgcacaccc acacttctcc ttctccttct cctcatactc ctccacaacc ccatcatcaa 120caaccgcaac acctccataa ccaaaaccaa ctacaaccca acatgggtga gaataaagca 180gcagaaatca aagactttca gattgtagta gctgacaaag aagagcaaaa gaaacagtta 240gctccaaaaa gaagctcaaa caaagacaga cacacaaaag ttgaaggtag aggtagaagg 300ataaggatgc cagctctttg tgcagcaaga atctttcaat taacaagaga attgggtcac 360aaatctgatg gtgagacaat acagtggctt ctacaacaag ctgaaccatc tataattgca 420gcaactggga ctggtacaat acctgcatca gctttggcag ctgctggtgg tgcaatttca 480caacaaggag cttctctttc agctggtttg catcaaaaga ttgatgatat aggtgagtcc 540agtagtagga ggaccagttg ggcaatgtta ggtggcaatt tagggagacc ccatcaagtt 600acaagtgcag gattatggcc cccagttgga ggttatgggt tccagtcatc atctaatacc 660actggtccat caacaacaaa tatagtaagt gaaggtggtg gtggctcgag ttatttgcaa 720aaaaatggat tttcagggtt tgacatgcca ggaaacaata ttgggcctat gagtcttact 780tcaattttag gtgtgggtag ccagcagtta ccaggattgg agcttgggtt gtcacaagat 840gggcatattg gggttttgag cccacaagct ttgagtcaga tttatcagca gatggggcag 900gctagggtgc agcagcacca gcagcaaaac ccttctaaag atgattcaca aggatcaggg 960cagtaa 9661112079DNAPopulus trichocarpa 111atgggcgagg aagcagaccc aacaatgaag aagaagaaga aaaagggacg tccttctctt 60ctagagcttc aaaaacgctc cctcaaacaa caacagctac agcaaacaac ccctatttct 120cttaaaaacc ctaatcctct caattccaat tctgccctcc ctaaccgtcg atccgctcgc 180cggagctcta attcctatgc accggagtgg attgacggag acgacgacga agacgacgac 240gaagacgatg agcgtaaaga gaagaagcat aagctcttgc gcggattgaa ttctcagaaa 300aataacaatc agaattccaa ttcgtcgagc ccttcgaatt tgcacggctc tgattcgaat 360gctggtggcg gtaatcaaga ggacggaatt agaaggcgca agatcagcgc cgtccgtctt 420ggatctgatg atttgggtga aaaggttttg aaagggacag acactattca tgggtcatct 480gtggagcctg gtcccactac acctttgcca gacaaaaagt tgttggtctt cattcttgac 540agacttcaaa agaaggacac ctatggggtt ttctctgagc cagtggatcc agaagagctt 600cctgattact ttgacatcgt tgagaatccc atggattttt ctacagtgag gaaaaagtta 660gatgagggag cctacgccca cttggaacaa tttgagaaag atgttctttt gatatgctca 720aatgcaatgc agtacaaccc ttccgatact atttacttcc gacaggcaag ggccatgcaa 780gagcttgcaa agaaggactt tgagaatctt aggcaagata gtgatgatag tgaaccacaa 840actaaggttg cgaggagagg gaggccacca gccctaggca agctgaaaaa ggcacttgag 900aggtctccaa ttgaccgtgt tggtcctgaa gcttcctcgg atgcaactct tgccaccgga 960ggagatcaca ataacttgtc caatggttac aatctcagaa aaagttcttc atacaagtac 1020cagcctggtg atgcatttgt ccgggcctct tacagcagtg aaaattattc tacctggtta 1080tctgaatggg aaaatgaatt tccagcatct gttgtaaagg ctgtgatgaa gtatggaaag 1140aaaccgtttg tgctagatga gaacaagcgt gacacctata agcatccatt gggctcccat 1200gagccatcca tcttatcgac ctttgaagga gaattaaagc aattagtggt ggtaggttta 1260agctctgagc acggttatgc aagaagtctg gctcgatttg ctgctgatct tggccctgtt 1320gtttggagaa ttgcttcgaa gaaaattgaa agtgttttgc caacaggact tgagtttggc 1380cctgggtggg taggagaaaa taaagcaatg gagaagcaaa agattttaaa caaccttgta 1440tctgataacc atttaagcag atttcaacct gctgcttctt caagtagaga ggcagcatgg 1500aatagagaag gcctgccaga aactgttggc gggttaaatc ctcaaaatga gttagctaca 1560ctgaacagtg gtgctggtgg gatgaaatcc atgccttctc tccagattca gcagaaacca 1620ataattcatc ctgatatgaa tggtttcagt ggaggatttg gatataactc ttcacctcaa 1680ccggggatgg cacgaactgt ggcaccgaca ggaaagctca atttggagca gactgcagtg 1740ccttcccaaa tgtttggtgt ggttccaact ggcaacagtg ccttcatttc agtgcctggg 1800aatgatttta atacaaataa aggtatgctg tcagaaactt caagtggatt attgcagcct 1860ggaatttctc cagccgtagg ctccagctct gactctcgta catttggcaa tgtggggttt 1920ggtggcaaat catcttggca gggattttta ccatatcagc aacaaggtac tgttccattt 1980ccaccagatc tgaatgttgg attcttggca cctggttcac ctacttcgag tgtgcccatc 2040ggttcaccac ggcagccaga tttagcattg cagctctga 20791123342DNAPopulus trichocarpa 112atgaagtcac cggccaacgg tgccgccgcc gcagtcacca acggtgaagg ggtagagaag 60aagagcataa accctgagtt atggcaagca tgtgcaggac cactagtgaa cttgccggcg 120gccggaactc atgtggtcta cttccctcaa gggcatagtg aacaggttgc agcatctttg 180aagaaggatg tgaatgcgca aattccaaac tacccaaatc ttccctcgaa gctgctatgt 240ctccttcaca atgtcacctt acatgcggac cctgaaacag atgaagttta tgttcagatg 300acacttcaac ctgtttcttc ttttgacaag gatgcattat tgaggtcaga tcttgcactg 360aagtcaaata aaccacagac agagtttttc tgtaaaacat tgacagcaag tgatacaagc 420actcatggag gtttctccgt gcctcgccgt gcagctgaaa agacattccc gcctcttgat 480ttctccatgc aaccacctgc tcaagaactt gtagctaggg atttgcatga taatgtttgg 540actttccggc atatctatcg tggacaacca aagcgtcact tgcttacaac tgggtggagc 600ttatttgtca gtgggaagag gctttttgcc ggtgactcag ttttatttat gagagatgaa 660aagcagcagc ttctattggg catcagaagg gctaacaggc aacctaccaa cttatcatcg 720tcggtattgt caagtgatag catgcatatt gggatcctag ctgctgcagc ccatgcagca 780gcaaacaata gcccctttac cgtgtactat aatccaaggg caagtccatc agaatttgtt 840atccctttag ccaagtatta caaggcagtg tacagcaacc aaatatcact tggcatgcgc 900ttccgcatga tgtttgaaac tgaagagtca ggaacaagaa ggcatatggg caccattact 960ggtatcagtg atcttgatgc tgtaagatgg aaaaactcac aatggcgtaa tttgcaggtt 1020ggttgggatg agtcaactgc tggtgaaaga cgcaatagag tctcgatctg ggagatcgaa 1080ccagtgactg ctccattttt catatgtcct cctccatttt ttagatctaa gcatccaagg 1140caaccgggaa tgccagatga tgattccact gattttgata gcctattcaa gaggactatg 1200ccttggcttg gtgatgatat ctacatgaaa gatccccagg ttctccctgg cctaagccta 1260gcccagcgaa tgaacatgca gcaaaaccct tcactggcaa actcaatgca gcccaattat 1320atgcaatcgt tgtcagggtc tgttctgcag aatcttcctg gaggtgatct ttcccgccag 1380ttaggcttgt catcacctca aatgcctcag ccaaacaact tacagttcaa tgcccaaagg 1440ctgcctcagc aagcacaaca gcttgatcag cttccaaagc tacagtcctt gctcaaccca 1500ctgggttcca tcatacagtc acagcaacag atgggtgaca tcactcaaca atcgaggcaa 1560aatatgatgg ctcaaactct accctcaagt caagttcaag cccaacttct gcagcctcaa 1620actcttgccc agaccaataa cattcttcag caacagccat ctattcaaag ccatcagctt 1680cttagaaacc ttccgcaaac cttgcaccag cagcagcaga atcaacagca acatattatg 1740ggtcagaacc agcagcaaag cctaatgcaa tctcagttgt ctgatcaagt aaaccaacat 1800atgcaaatgt ctgacaatca gattcagtct caactgatgc agaagcttca gcagcaacag 1860caatcagttt cagctcagca atctgctatg catcaggctg gtcaacttgg acagcttcaa 1920gattcacaaa ggcagctgct ggatgcatcc cagagttttt ctaggtccat gacacccagc 1980caaatgttgg aaatccctca aacaacaccc acttctctcc ctcagccaaa tactattcca 2040cagcagatga ctaagaataa caaccaaacc aatactcgat tctcacatct gcctcaacag 2100ctgaaacctc aacaacagca ttctggcatc atgctgttgt cagaaatggc tggtcacatg 2160ggacttccgc cgagctcaat ggccaatcag ctctccacag ctggtagcag tatattgact 2220gcagcagctg gaccaggaca gtctggtatc actgatgatg ttccttcctg ttctacttca 2280ccttccacaa acaattgtcc aaacatagtt caaccaatga tcaacggctg ggcccaccga 2340agcacagcaa tgggagagga catggctcag tctgccgtga cactctttag ccctagtgca 2400ttggaaaccg tgtcctctaa tggtaattta gttaaagatc tgctgcagaa atctgaggtt 2460aagccatcat tgaacatctc caagaatcaa aacccaggat tattttcctc gcaaacatac 2520ctaaatgggg tagctgccca gatagattat ttggacacat catcttctac aacttctgtt 2580tgcctatcac aaaatgatgt ccatttgcag cagaataaca attcactgtc atataatccc 2640caatcagtgt tgttgagaga cgcaagccat gatggtgagc tccagggaga tccgaggaat 2700aatattttat atgggacaaa cattgatagc caacttgtga tgccaataaa ttctgaccat 2760ttattaacaa agggcatgat ggggctgggg aaggacttct caaataattt ctcttcagga 2820ggcatgctta caaattgtga aaattccaaa gatcctcaac aggagctttc atcggcaatt 2880gtttccaagt catttggagt tccagatatg ccattcaatt caattgactc aacaatcaat 2940gacagtagct tattgaatag aggttcttgg gctcctccac agcaacagtt tcagcgaatg 3000cgaacatata caaaggtgta caagcgtgga gctgtaggaa ggtcaattga cataacgcgg 3060tattcaggtt atgacgagct taagcaggat ctggctcgta ggtttggtat agagggacag 3120ttggaagatc aacaaaggat aggctggaaa cttgtttaca ctgatcacga gaatgatgtc 3180ctgctagtgg gggatgatcc ttgggaagag tttgtgaact gtgtccgctg catcaagatc 3240ctgtctcctc aagaagtcca acagatgagc ttggatggag attttggcaa ctctgtcctt 3300cctaatcaag ccggcagtag ttctgataat gtcaatgcat aa 33421131506DNAPopulus trichocarpa 113atggtgggtg gtgagaattt atccagaatc acagtatcta aagcttctgg agaagacaca 60attagcaatg tttcttcttt tggtaaccaa ggcatgccac actctattac agtgccacca 120ccaaagaaga agcgaaatct ccctggaatg ccagatccag atgcagaagt cgttgcgtta 180tcaccaaaga ctttagtagc cacaatcaga ttcgtgtgtg agatctgtaa caagggcttt 240caaagagacc agaacctcca acttcacaga cgaggccata acttaccatg gaagctaaag 300caacgaacca acaaagagcc aaggaagcgt gtctatgttt gtccagagtc atcttgtgta 360catcacaacc cagctagagc tcttggtgat cttactggca ttaaaaagca cttctacaga 420aagcatggtg agaaaaagtg gaagtgtgag agatgttcga agaaatatgc cgtccagtct 480gattggaaag ctcacttgaa aacttgtggc actaaagagt ataaatgtga ttgtggcact 540ttgttttcaa ggagggatag ttttgtaaca cacagagcat tttgcgatgc gttagcggag 600gagagtgcaa gggcgcaaac cctagcgata atgggaaggg aagggaatgg ttgtgacatc 660aagcgtgtgg gtgcttcacc accgcctcca ccacttacgc cgtctactag tgtggtgtct 720ccgggtttat cagttcaaag ctcagaatta gcagaaaatc caattggact ttcaccacca 780ccaatagcat gtgcctcggc tacaagcaca agctcaacta gctcaaccag taatgtattt 840gcgactacat ttgcctcttc gacagcaaca ccagctgcta tcccacaaca agcatcagta 900ccatcctctt tcccaaatct gttttgcggc ttagctcgct ctgattatcc cactactatg 960ccaacaccta gagcgataga acccccatca ctctctcttt caccatcttt ttatctctcc 1020aacaatactt cgtctctctt cagcacagag caagagcact accattacac accatctccg 1080caaccagcca tgtctgccac tgcattgctt caaaaagcag cacaaatggg tgcaacgaca 1140tcaaacccat catttctccg tggtttagga ttaccccgtt caactaatca agacagtaat 1200tgcaataaat gggatgtgaa gccagagaac aacaccactg ttgcagctgg actcggcctt 1260gggcttccct ccagtgatgt tatgatgggt tcatcttcat tgtttggaaa caaaccagcg 1320acacttgacc ttctcgggct tggcatggat gctgctagtt ctgctttgct aaattcctat 1380agtggtggct ttaatgttgg agcagccact gccgcagcat atggtggagg aggagggaga 1440gggacttctg aagaaacatg ggatggtgtg cctgagagga agccttatgg atctacagga 1500gcttga 1506114966DNAPopulus trichocarpa 114atggatccca agggctctaa ctcaaaaaac ccacatgagc tacccacttt ctttaccctt 60acgcacaccc acacttctcc ttctccttct cctcatactc ctccacaacc ccatcatcaa 120caaccgcaac acctccataa ccaaaaccaa ctacaaccca acatgggtga gaataaagca 180gcagaaatca aagactttca gattgtagta gctgacaaag aagagcaaaa gaaacagtta 240gctccaaaaa gaagctcaaa caaagacaga cacacaaaag ttgaaggtag aggtagaagg 300ataaggatgc cagctctttg tgcagcaaga atctttcaat taacaagaga attgggtcac 360aaatctgatg gtgagacaat acagtggctt ctacaacaag ctgaaccatc tataattgca 420gcaactggga ctggtacaat acctgcatca gctttggcag ctgctggtgg tgcaatttca 480caacaaggag cttctctttc agctggtttg catcaaaaga ttgatgatat aggtgagtcc 540agtagtagga ggaccagttg ggcaatgtta ggtggcaatt tagggagacc ccatcaagtt 600acaagtgcag gattatggcc cccagttgga ggttatgggt tccagtcatc atctaatacc 660actggtccat caacaacaaa tatagtaagt gaaggtggtg gtggctcgag ttatttgcaa 720aaaaatggat tttcagggtt tgacatgcca ggaaacaata ttgggcctat gagtcttact 780tcaattttag gtgtgggtag ccagcagtta ccaggattgg agcttgggtt gtcacaagat 840gggcatattg gggttttgag cccacaagct ttgagtcaga tttatcagca gatggggcag 900gctagggtgc agcagcacca gcagcaaaac ccttctaaag atgattcaca aggatcaggg 960cagtaa 966115690DNAPopulus trichocarpa 115atggaaccat cgtcgtcgtc agcagtagca gcagcaatag tgagtactaa cgccaacatc 60aacacggatc gaacaagaag gaaaaagaag aagaaatcag tgttgcagca acaccaatca 120aaacagaacc aaaactcaca gagccacgcc aaatggaaaa cagaagcaca acaacaagtc 180tactcatcca aactcatcca agccttaagc caagtcaatc tcaacccttc aacttcatca 240gccccgcgtc aaggtcgagc cgttagagaa gttgctgatc gggctttagc tttcgctgct 300aaaggtaaaa ccaggtggag ccgagccatt ttaactagcc gcatcaaact caaatttcgg 360aaacaacaac ataagagaca gagacttgcg tcgtcgtctt cttcttctcc aggatccact 420accgggagta gcagccggtc gtcgaggaag cataaagtga gtgttttgag gttgaaagcg 480aagggtttgc cggctgttca aaggaaagtt cgtgttcttg gccggttagt tcctggttgc 540ctgaaacaac cattgcctgt tattttggaa gaagctacag attatattgc tgctttggag 600atgcaagtta aaaccatgac tgctatagct gagcttcttt ctcgctctac ctccgaagcc 660agctctactt ctgagccgat gacctcctaa 690116187PRTPopulus trichocarpa 116Met Gln Pro Gly Leu Asp Glu Ile Thr Met Thr Ala Trp Lys Asn Leu 1 5 10 15 Lys Gln Arg Leu Ser Phe Lys Gly Leu Gly Ser Cys Cys Gly Ser Thr 20 25 30 Ser Trp Ser Ser Arg Ser Ala Thr Pro Thr Met Pro Phe Ile Asp Ile 35 40 45 Glu Gln Glu Glu Glu Glu Glu Pro Ile Met Gln Asn Gln Ala Gln Arg 50 55 60 Gly Gly Gly Ala Ala Ala Ala Ala Ala Ala Pro Gly Ala Gly Met Asn 65 70 75 80 Leu Ala Met Ala Leu Ala Ala Glu Arg Asn Leu Gly Asp Ser Asn Val 85 90 95 Lys Thr Leu Met Ser Leu Ile Glu Glu Thr Asp Gly Val Asp Trp Arg 100 105 110 Lys Lys Asn Asn Ser Asn Asp Lys Ser Arg Arg Asp Lys Glu Gln Glu 115 120 125 Gln Lys Gln Glu Glu Glu Lys Asp Trp Val Cys Cys Val Cys Met Glu 130 135 140 Arg Asn Lys Gly Ala Ala Phe Ile Pro Cys Gly His Thr Phe Cys Arg 145 150 155 160 Val Cys Ser Arg Glu Met Trp Val Asn Arg Gly Cys Cys Pro Ile Cys 165 170 175 Asn Arg Ser Ile Leu Asp Ile Leu Asp Ile Phe 180 185 117214PRTPopulus trichocarpa 117Met Ala Arg Gly Lys Val Gln Leu Lys Arg Ile Glu Asn Ala Thr Ser 1 5 10 15 Arg Gln Val Thr Phe Ser Lys Arg Lys Asn Gly Leu Leu Lys Lys Ala 20 25 30 Tyr Glu Leu Ser Ile Leu Cys Asp Ala Glu Val Ala Val Ile Ile Phe 35 40 45 Ser Gln Lys Gly Thr Leu Phe Lys Phe Ala Ser Ile Asp Gln Ile Gln 50 55 60 Lys Thr Ile Asp Arg Tyr Arg Lys Asn Ala Lys Gln Leu His Thr Asp 65 70 75 80 Arg Ile Asp Val Glu Gln Ser Lys Glu Gln Leu Arg Gln Glu Ser Ala 85 90 95 Asn Met Ala Lys

Lys Ile Glu Ile Ile Glu Ile Leu Gln Arg Lys Leu 100 105 110 Leu Gly Gln Asp Leu Asp Ser Cys Ser Pro Glu Glu Leu His Asp Ile 115 120 125 Asp Asn Gln Leu Glu Ile Ser Leu Ser Asn Ile Arg Ala Arg Lys Thr 130 135 140 Gln Leu Phe Lys Glu Gln Ile Glu Gln Leu Gln Ala Lys Glu Arg Leu 145 150 155 160 Leu Leu Met Glu Asn Ala Arg Leu Thr Lys Gln Cys Asp Ala Gln Pro 165 170 175 Leu Gln Gln Ser Thr Gln Ser Asn Gln Val Val Ser Tyr Leu Thr Ser 180 185 190 Cys Ser Lys Ser Ser Asp Ile Val Glu Thr Asp Leu Tyr Ile Gly Leu 195 200 205 Pro His Met Arg Cys Leu 210 118584PRTPopulus trichocarpa 118Met Ala Tyr Met Cys Ala Asp Ser Gly Asn Leu Met Ala Ile Ala Gln 1 5 10 15 Gln Val Ile Lys Gln Lys Gln Gln Gln Glu Gln Gln Gln Gln Gln Ser 20 25 30 His His His His His Asn Gln Gln Gln Gln Phe Leu Gly Leu Asn Pro 35 40 45 Phe Ser Leu Asn Pro Trp Pro Thr Asn Ile Met Ser Ala Asn Pro Asn 50 55 60 Leu Gly Tyr Gly Leu Ser Gly Pro Ala Ala Phe Ser Asp Pro Phe Gln 65 70 75 80 Ser Gly Gln Glu Thr Gly Asp Pro Pro Val Phe Ser Phe Ser Asn Met 85 90 95 Glu Gln His His Ser Ser Gly Phe Arg Leu Pro Tyr Phe Thr Gly Ala 100 105 110 Gly Gly Glu Phe Asp Ser Asp Glu Trp Met Asp Ser Leu Met Asn Gly 115 120 125 Gly Asp Ser Thr Asp Ser Ser Asn Leu Pro Ser Gly Cys Asp Ala Trp 130 135 140 Gln Asn Ser Thr Asp Phe Gly Met Tyr Thr Ser Asp Pro Phe Asn Thr 145 150 155 160 Cys Pro Ser Arg Leu Thr Val Gly Cys Ser Pro Pro Ser Asp Leu Asn 165 170 175 Gly Val Leu Ser Asn Ser Leu Trp Val Ala Pro Ser Pro Pro Gln Glu 180 185 190 Ile Lys Pro Thr Thr Ser Pro Pro Pro Leu Leu Pro Pro Thr Val Lys 195 200 205 Asn Glu Thr Val Gly Gly Ser Glu Glu Ile Val Gln Leu Ser Ser Ser 210 215 220 Pro Val Leu Lys Ala Leu Val Glu Cys Ala Gln Leu Val Glu Ser Lys 225 230 235 240 Ala Asp Gln Ala Val Lys Ser Leu Val Lys Cys Lys Asp Leu Val Ser 245 250 255 Glu Asn Gly Asp Pro Val Glu Arg Ala Gly Phe Tyr Phe Ala Glu Gly 260 265 270 Leu Cys Arg Arg Val Ala Val Gly Glu Leu Asp Val Leu Lys Asn Phe 275 280 285 Asp Gln Thr Ser Glu Glu Phe Thr Leu Ser Tyr Lys Ala Leu Asn Asp 290 295 300 Ala Cys Pro Tyr Ser Lys Phe Ala His Leu Thr Ala Asn Gln Ala Ile 305 310 315 320 Leu Glu Ala Thr Glu Lys Ala Ser Lys Ile His Ile Val Asp Phe Gly 325 330 335 Ile Val His Gly Val Gln Trp Ala Ala Leu Leu Gln Ala Leu Ala Thr 340 345 350 Arg Ser Ala Gly Lys Pro Val Arg Ile Arg Ile Ser Gly Ile Pro Ala 355 360 365 Pro Val Leu Gly Lys Asn Pro Ala Ala Ser Leu Leu Ala Thr Gly Asn 370 375 380 Arg Leu Leu Asp Tyr Ala Lys Leu Leu Gly Leu Asn Phe Glu Phe Glu 385 390 395 400 Pro Ile Leu Thr Pro Ile Gln Glu Leu Asn Glu Ser Cys Phe Arg Ala 405 410 415 Glu Pro Asp Glu Val Leu Ala Val Asn Phe Met Leu Gln Leu Tyr Asn 420 425 430 Leu Leu Asp Glu Ser Pro Val Ala Val Glu Thr Ala Leu Lys Met Ala 435 440 445 Lys Ser Leu Asn Pro Ile Ile Val Thr Leu Gly Glu Tyr Glu Ala Ser 450 455 460 Leu Asn Arg Val Gly Tyr Leu Thr Arg Phe Lys Asn Ala Leu Arg Tyr 465 470 475 480 Tyr Thr Ala Val Phe Glu Ser Leu Glu Pro Asn Met Ser Arg Asp Ser 485 490 495 Pro Glu Arg Leu Gln Val Glu Arg Leu Leu Leu Gly Arg Glu Phe Leu 500 505 510 Val Leu Glu Arg Met Glu Asp Lys Asp Gln Trp Gly Val Leu Met Glu 515 520 525 Ser Ser Gly Phe Glu Ser Val Ser Leu Ser His Tyr Ala Met Ser Gln 530 535 540 Ala Lys Ile Leu Leu Trp Tyr Cys Asn Tyr Ser Asp Leu Tyr Ser Leu 545 550 555 560 Asp Asp Ser Gln Pro Gly Phe Leu Thr Leu Ala Trp Asn Glu Val Pro 565 570 575 Leu Leu Thr Val Ser Ser Trp Arg 580 119242PRTPopulus trichocarpa 119Met Gly Arg Gly Arg Val Glu Leu Lys Arg Ile Glu Asn Lys Ile Asn 1 5 10 15 Arg Gln Val Thr Phe Ala Lys Arg Arg Asn Gly Leu Leu Lys Lys Ala 20 25 30 Tyr Glu Leu Ser Val Leu Cys Asp Ala Glu Val Ala Leu Ile Ile Phe 35 40 45 Ser Asn Arg Gly Lys Leu Tyr Glu Phe Cys Ser Ser Ser Ser Met Leu 50 55 60 Lys Thr Leu Glu Arg Tyr Gln Lys Cys Asn Tyr Gly Ala Pro Glu Pro 65 70 75 80 Asn Val Ser Ala Arg Glu Ala Leu Glu Leu Ser Ser Gln Gln Glu Tyr 85 90 95 Leu Lys Leu Lys Ala Arg Tyr Glu Ala Leu Gln Arg Thr Gln Arg Asn 100 105 110 Leu Leu Gly Glu Glu Leu Gly Pro Leu Ser Ser Lys Glu Leu Glu Ser 115 120 125 Leu Glu Arg Gln Leu Asp Met Ser Leu Lys Gln Ile Arg Ser Thr Arg 130 135 140 Thr Gln Tyr Met Leu Asp Gln Leu His Asp Leu Gln His Lys Glu His 145 150 155 160 Met Leu Thr Ala Ala Asn Lys Ser Leu Lys Glu Arg Leu Met Glu Gly 165 170 175 Tyr Gln Leu Asn Ser Leu Gln Leu Asn Pro Ser Ala Glu Asp Val Glu 180 185 190 Tyr Ala Arg Gln Gln Ala Gln Pro Gln Gly Asp Gly Phe Phe His Ala 195 200 205 Leu Glu Cys Glu Pro Thr Leu Gln Ile Gly Tyr Gln Pro Glu Asn Ile 210 215 220 Thr Met Val Thr Ala Gly Pro Ser Met Thr Thr Tyr Met Pro Gly Trp 225 230 235 240 Leu Ala 120585PRTPopulus trichocarpa 120Met Ser Leu Val Gly Pro Ala Glu Leu Ser Ala Thr Pro Tyr Gly Asn 1 5 10 15 His Lys Leu Tyr Ser Leu Lys Gly Ser Asn Asp Asn Ser Gly Leu Ser 20 25 30 Ala Gln Ile Phe Cys Pro Asp Lys Arg Gln Asn Met Tyr Met Thr Asp 35 40 45 Ser Tyr Ser Ser Glu Ser Tyr Glu Lys Tyr Phe Leu Asp Ser Pro Thr 50 55 60 Glu Glu Leu Ile Gln Pro Ser Ser Ser Gly Ile Ser Gly Asn Ser Ala 65 70 75 80 Pro Pro Gln Gly Thr Ser Ser Tyr Gln Leu Arg Lys Asn Leu Gly Pro 85 90 95 Ser Met Ser Pro Gln Asp Asp Pro Tyr Asp Ala Cys Phe Thr Leu Thr 100 105 110 Thr Pro Cys Asp Gly Tyr Gln Phe Asn Ser Glu Ser Asp Tyr Leu Asp 115 120 125 Ile Glu Ser Pro Asp Pro Leu Asn Tyr Asp Glu Tyr Lys Met Lys Leu 130 135 140 Lys Phe Gln Glu Leu Glu Arg Ala Leu Leu Asn Asp Asn Asp Glu Asp 145 150 155 160 Gly Met Phe Gly Asn Ser Gln Ser Met Glu Met Asp Gly Glu Trp Ser 165 170 175 Asp Pro Ile Gln Asn Gly Met Leu His Asp Ser Pro Lys Glu Ser Ser 180 185 190 Ser Ser Asp Ser Ser Leu Ser Ser Ile Ser Ser Asn Lys Glu Val Ser 195 200 205 Gln Leu Ser Pro Arg Thr Pro Arg Arg Leu Leu Phe Glu Cys Ala Asn 210 215 220 Ala Ile Ser Glu Gly Asn Ile Glu Lys Ala Ser Thr Leu Ile Asn Glu 225 230 235 240 Leu Arg Gln Leu Val Ser Ile Gln Gly Asp Pro Pro Gln Arg Ile Ala 245 250 255 Ala Tyr Met Val Glu Gly Leu Ala Ala Arg Met Ala Glu Ser Gly Lys 260 265 270 Tyr Leu Tyr Lys Ala Leu Lys Cys Lys Glu Pro Pro Ser Ser Asp Arg 275 280 285 Leu Ala Ala Met Gln Ile Leu Phe Glu Ile Cys Pro Cys Phe Lys Phe 290 295 300 Gly Phe Met Ala Ala Asn Gly Ala Met Ile Glu Ala Phe Lys Gly Glu 305 310 315 320 Arg Arg Val His Ile Ile Asp Phe Asp Ile Asn Gln Gly Ser Gln Tyr 325 330 335 Ile Thr Leu Ile Gln Thr Leu Ala Asn His Gln Gly Lys Leu Pro His 340 345 350 Leu Arg Leu Thr Gly Val Asp Asp Pro Glu Ser Val Gln Arg Pro Val 355 360 365 Gly Gly Leu Arg Ile Ile Gly Gln Arg Leu Glu Lys Leu Ala Glu Ala 370 375 380 Tyr Lys Val Ser Phe Glu Phe His Ala Val Ala Ser Lys Thr Ser Leu 385 390 395 400 Val Asn Pro Ser Met Leu Asn Cys Lys Pro Gly Glu Ala Leu Ile Val 405 410 415 Asn Phe Ala Phe Gln Leu His His Met Pro Asp Glu Ser Val Ser Thr 420 425 430 Val Asn Glu Arg Asp Gln Leu Leu Arg Met Ala Lys Ser Leu Asn Pro 435 440 445 Lys Leu Val Thr Val Val Glu Gln Asp Val Asn Thr Asn Thr Ala Pro 450 455 460 Phe Phe Pro Arg Phe Thr Glu Ala Tyr Asn Tyr Tyr Ser Ala Val Phe 465 470 475 480 Asp Ser Leu Asp Ala Thr Leu Pro Arg Glu Ser Gln Asp Arg Leu Asn 485 490 495 Val Glu Lys Gln Cys Leu Ala Arg Asp Ile Val Asn Ile Val Ala Cys 500 505 510 Glu Gly Glu Glu Arg Ile Glu Arg Tyr Glu Val Ala Gly Lys Trp Arg 515 520 525 Ala Arg Met Met Met Ala Gly Phe Thr Ser Cys Ser Ile Thr Pro Asn 530 535 540 Val Val Asp Met Ile Arg Lys Leu Ile Lys Glu Tyr Cys Asp Arg Tyr 545 550 555 560 Met Leu Lys Gln Glu Val Gly Ala Leu His Phe Gly Trp Glu Asp Lys 565 570 575 Ser Leu Ile Val Ala Ser Ala Trp Lys 580 585 121544PRTPopulus trichocarpa 121Met Lys Arg Asp His Gln Glu Thr Ile Gly Gly Gly Ile Gly Asn Arg 1 5 10 15 Ala Glu Ser Ser Ser Ser Ser Met Glu Thr Gly Lys Gly Lys Ser Trp 20 25 30 Val Glu Asp Asp Gln Asp Ala Gly Gly Met Asp Glu Leu Leu Ala Val 35 40 45 Leu Gly Tyr Lys Ile Lys Ser Ser Asp Met Ala Asp Val Ala Gln Lys 50 55 60 Leu Glu Gln Leu Glu Met Val Leu Gly Ser Glu Asp Gly Ile Ser His 65 70 75 80 Leu Ala Ser Asp Thr Val His Tyr Asn Pro Ser Asp Leu Ser Gly Trp 85 90 95 Val Gln Asn Ser Leu Leu Gly Gln Pro Ser Thr Ile Thr Pro Leu Asp 100 105 110 Phe Pro Ser Asn Ser Gln Ser Lys Val Phe Ala Asp Asp Ser Glu Tyr 115 120 125 Asp Leu Arg Ala Ile Pro Gly Val Ala Ala Tyr Pro Gln Gln Glu Leu 130 135 140 Asp Lys Thr Met Ala Val Ser Gly Thr Leu Ser Glu Pro Thr Arg Pro 145 150 155 160 Val Val Leu Val Asp Ser Gln Glu Thr Gly Val Arg Leu Val His Thr 165 170 175 Leu Leu Ala Cys Ala Glu Ala Ile Gln Gln Glu Asn Leu Lys Leu Ala 180 185 190 Asp Ala Leu Val Lys His Ile Gly Leu Leu Ala Ala Ser Gln Thr Gly 195 200 205 Ala Met Arg Lys Val Ala Thr Tyr Phe Ala Glu Ala Leu Ala Arg Arg 210 215 220 Ile Tyr Lys Ile Phe Pro Gln Asp Tyr Cys Leu Asp Ser Ser Cys Ser 225 230 235 240 Asp Thr Leu Glu Met His Phe Tyr Glu Thr Cys Pro Tyr Leu Lys Phe 245 250 255 Ala His Phe Thr Ala Asn Gln Ala Ile Leu Glu Ala Phe Ala Asn Ala 260 265 270 Ser Arg Val His Val Ile Asp Phe Gly Leu Lys Gln Gly Met Gln Trp 275 280 285 Pro Ala Leu Met Gln Ala Leu Ala Leu Arg Pro Gly Gly Pro Pro Ala 290 295 300 Phe Arg Leu Thr Gly Ile Gly Pro Pro Gln Pro Asp Asn Thr Asp Ala 305 310 315 320 Leu Gln Gln Val Gly Trp Lys Leu Ala Gln Leu Ala Gln Thr Ile Gly 325 330 335 Val Glu Phe Glu Phe Arg Gly Phe Val Ala Asn Ser Leu Ala Asp Leu 340 345 350 Asp Ala Glu Met Leu Gly Leu Leu Pro Pro Glu Val Glu Ala Val Ala 355 360 365 Val Asn Ser Val Phe Glu Leu His Arg Leu Leu Gly Arg Pro Gly Gly 370 375 380 Ile Asp Lys Val Leu Glu Ser Ile Lys Ala Met Arg Pro Lys Ile Val 385 390 395 400 Thr Ile Val Glu Gln Glu Ala Asn His Asn Gly Pro Val Phe Leu Asp 405 410 415 Arg Phe Thr Glu Ala Leu His Tyr Tyr Ser Ser Leu Phe Asp Ser Leu 420 425 430 Glu Gly Ser Gly Leu Thr Pro Pro Ser Gln Asp Leu Val Met Ser Glu 435 440 445 Leu Tyr Leu Gly Arg His Ile Cys Asn Val Val Ala Cys Glu Gly Ala 450 455 460 Asp Arg Val Glu Arg His Glu Thr Leu Ala Gln Trp Arg Thr Arg Phe 465 470 475 480 Asp Ser Ala Gly Phe Asp Pro Val His Leu Gly Ser Asn Ala Phe Lys 485 490 495 Gln Ala Ser Met Leu Leu Ala Leu Phe Ala Gly Gly Asp Gly Tyr Arg 500 505 510 Val Glu Glu Asn Asn Gly Cys Leu Met Leu Gly Trp His Thr Arg Pro 515 520 525 Leu Ile Ala Thr Ser Ala Trp Gln Leu Ala Ala Gly Asp Ser Gln Gln 530 535 540 122352PRTPopulus trichocarpa 122Met Glu Asn Asn Cys Val Tyr Ser Glu Glu Asp Glu Gln Met Glu Leu 1 5 10 15 Pro Pro Gly Phe Arg Phe His Pro Thr Asp Glu Glu Leu Ile Thr His 20 25 30 Tyr Leu Ser Gln Lys Val Leu Asp Asn Tyr Phe Cys Ala Arg Ala Ile 35 40 45 Gly Glu Val Asp Leu Asn Lys Cys Glu Pro Trp Asp Leu Pro Trp Arg 50 55 60 Ala Lys Met Gly Glu Lys Glu Trp Tyr Phe Phe Cys Val Ile Asp Arg 65 70 75 80 Lys Tyr Pro Thr Gly Leu Arg Thr Asn Arg Ala Thr Asp Ala Gly Tyr 85 90 95 Trp Lys Ala Thr Gly Lys Asp Lys Glu Ile Tyr Arg Ala Lys Thr Leu 100 105 110 Val Gly Met Lys Lys Thr Leu Val Phe Tyr Lys Gly Arg Ala Pro Lys 115 120 125 Gly Glu Lys Thr Asn Trp Val Met His Glu Tyr Arg Leu Glu Gly Lys 130 135 140 Asn Pro Val Tyr Asn Pro Pro Lys Thr Ala Lys Asn Asp Trp Val Ile 145 150 155 160 Cys Arg Ile Phe Glu Lys Ser Cys Gly Gly Lys Lys Thr His Ile Ser 165 170 175 Gly Leu Val Arg Leu Ser Ser Tyr Gly Asn Glu Leu Lys Pro Thr Ile 180 185 190 Leu Pro Pro Leu Met Asp Ser Ser Gln His Asn Asn Asp Lys Arg Thr 195 200 205 Asn Ile Gly Asp Lys Ser His Val Thr Cys Phe Ser Asn Pro Thr Glu 210

215 220 Asp Gln Lys Pro His Glu Thr Ile Ala Asp Cys Phe Asn Ile Ser Leu 225 230 235 240 Arg Ala Pro Leu Ser Ser Ser Asn Met Ser Pro Ser Ser Val Leu Phe 245 250 255 Ser Lys Pro Ser Pro Pro Asn Ser Phe Tyr Ser Ser His Ile Leu Pro 260 265 270 Asn Ile Ala Asn Phe Gln Tyr Pro Asp Ser Val Met Met Pro Glu His 275 280 285 Ser Met Leu Arg Ile Leu Leu Glu Asn Gln Gly Pro Gly Met Asn Leu 290 295 300 Asn Ser Lys Arg Glu Leu Ser Glu Asp Thr Gly Leu Ser Thr Asp Met 305 310 315 320 Ser Ser Val Val Thr Asn His Glu Leu Val His Gly Ser Phe Glu Asp 325 330 335 Pro Ser Ser Ser Ala Gly Pro Val Asp Leu Asp Tyr Leu Trp Asn Tyr 340 345 350 123820PRTPopulus trichocarpa 123Met Ser Phe Gly Gly Phe Leu Glu Asn Thr Ser Pro Gly Gly Gly Gly 1 5 10 15 Ala Arg Ile Val Ala Asp Ile Leu Tyr Asn Asn Asn Asn Asn Met Pro 20 25 30 Thr Gly Ala Ile Ala Gln Thr Arg Leu Val Ser Pro Ser Ile Thr Lys 35 40 45 Ser Met Phe Asn Ser Pro Gly Leu Ser Leu Ala Leu Gln Gln Pro Asn 50 55 60 Ile Asp Gly Gln Gly Asp Ile Thr Arg Met Ala Glu Asn Phe Glu Thr 65 70 75 80 Ser Val Gly Arg Arg Ser Arg Glu Glu Glu His Glu Ser Arg Ser Gly 85 90 95 Ser Asp Asn Met Asp Gly Ala Ser Gly Asp Asp Gln Asp Ala Ala Asp 100 105 110 Asn Pro Pro Arg Lys Lys Arg Tyr His Arg His Thr Pro Gln Gln Ile 115 120 125 Gln Glu Leu Glu Ala Leu Phe Lys Glu Cys Pro His Pro Asp Glu Lys 130 135 140 Gln Arg Leu Glu Leu Ser Arg Arg Leu Cys Leu Glu Thr Arg Gln Val 145 150 155 160 Lys Phe Trp Phe Gln Asn Arg Arg Thr Gln Met Lys Thr Gln Leu Glu 165 170 175 Arg His Glu Asn Ser Leu Leu Arg Gln Asp Asn Asp Lys Leu Arg Ala 180 185 190 Glu Asn Met Ser Ile Arg Asp Ala Met Arg Asn Pro Ser Cys Ser Asn 195 200 205 Cys Gly Gly Pro Ala Ile Ile Gly Asp Met Ser Leu Glu Glu Gln His 210 215 220 Leu Arg Ile Glu Asn Ala Arg Leu Lys Asp Glu Leu Asp Arg Val Cys 225 230 235 240 Ala Leu Ala Gly Lys Phe Leu Gly Arg Pro Ile Ser Ser Leu Ala Ser 245 250 255 Ser Leu Ser Pro Pro Thr Asn Ser Ser Leu Glu Leu Ala Val Gly Ser 260 265 270 Asn Gly Phe Ala Gly Leu Ser Thr Ile Ala Thr Thr Leu Pro Leu Gly 275 280 285 Pro His Phe Glu Gly Gly Ile Ser Gly Ala Leu Ser Met Val Thr Gln 290 295 300 Thr Arg Leu Ala Thr Ala Gly Val Thr Gly Ile Asp Arg Ser Val Glu 305 310 315 320 Arg Ser Met Phe Leu Glu Leu Ala Leu Ala Ala Met Asp Glu Leu Val 325 330 335 Lys Met Val Gln Thr Asp Glu Pro Leu Trp Ile Gly Ser Phe Glu Gly 340 345 350 Gly Arg Glu Ile Leu Asn His Glu Gly Tyr Leu Arg Thr Phe Thr Pro 355 360 365 Cys Ile Gly Met Lys Pro Ser Gly Phe Val Ser Glu Ala Ser Arg Glu 370 375 380 Thr Gly Met Val Ile Ile Asn Ser Leu Ala Leu Val Glu Thr Leu Met 385 390 395 400 Asp Ser Asn Arg Trp Ala Glu Met Phe Pro Cys Met Ile Ala Arg Thr 405 410 415 Ser Thr Thr Asp Val Ile Ala Ser Gly Met Gly Gly Thr Arg Asn Gly 420 425 430 Ser Leu Gln Leu Met Gln Ala Glu Leu His Val Leu Ser Pro Leu Val 435 440 445 Pro Val Arg Glu Val Asn Phe Leu Arg Phe Cys Lys Gln His Ala Glu 450 455 460 Gly Val Trp Ala Val Val Asp Val Ser Ile Asp Thr Ile Arg Asp Thr 465 470 475 480 Ser Gly Ala Pro Pro Thr Phe Val Asn Cys Arg Arg Leu Pro Ser Gly 485 490 495 Cys Val Val Gln Asp Met Pro Asn Gly Tyr Ser Lys Val Thr Trp Val 500 505 510 Glu His Ala Gln Tyr Asp Glu Arg Gln Ile His Gln Leu Tyr Arg Pro 515 520 525 Val Ile Ser Ser Gly Met Gly Phe Gly Ala Gln Arg Trp Ile Ala Thr 530 535 540 Leu Gln Arg Gln Cys Glu Cys Leu Ala Ile Leu Leu Ser Ser Asn Val 545 550 555 560 Pro Ser Arg Asp His Thr Ala Ile Thr Thr Ser Gly Arg Arg Ser Met 565 570 575 Leu Lys Leu Ala Gln Arg Met Thr Asp Asn Phe Cys Ala Gly Val Cys 580 585 590 Ala Ser Thr Val His Lys Trp Asn Lys Leu Asn Ala Gly Asn Val Asp 595 600 605 Glu Asp Val Arg Val Met Thr Arg Lys Ser Val Asp Asp Pro Gly Glu 610 615 620 Pro Pro Gly Ile Val Leu Ser Ala Ala Thr Ser Val Trp Leu Pro Val 625 630 635 640 Ser Pro Gln Arg Leu Phe Asp Phe Leu Arg Asn Glu Arg Leu Arg Ser 645 650 655 Glu Trp Asp Ile Leu Ser Asn Gly Gly Pro Met Gln Glu Met Ala His 660 665 670 Ile Ala Lys Gly Gln Asp His Gly Asn Cys Val Ser Leu Leu Arg Ala 675 680 685 Ser Ala Met Asn Ala Asn Gln Ser Ser Met Leu Ile Leu Gln Glu Thr 690 695 700 Cys Ile Asp Ala Ala Gly Ser Leu Val Val Tyr Ala Pro Val Asp Thr 705 710 715 720 Pro Ala Met His Val Val Met Asn Gly Gly Asp Ser Ala Tyr Val Ala 725 730 735 Leu Leu Pro Ser Gly Phe Ala Ile Val Pro Asp Gly Pro Gly Ser Arg 740 745 750 Asp Pro Pro Ser Thr Asn Gly Gly Pro Thr Ala Asn Asn Val Gly Gly 755 760 765 Gln Glu Arg Val Ser Gly Ser Leu Leu Thr Val Ala Phe Gln Ile Leu 770 775 780 Val Asn Ser Leu Pro Thr Ala Lys Leu Thr Val Glu Ser Val Glu Thr 785 790 795 800 Val Asn Asn Leu Ile Ser Cys Thr Val Gln Lys Ile Lys Ala Ala Leu 805 810 815 Gln Cys Glu Ser 820 124210PRTPopulus trichocarpa 124Met Ala Pro Lys Lys Leu Asn Gly Asn Asp Asn Gly Ser Leu Lys Lys 1 5 10 15 Ala Ser Gly Asp His Asp Lys Lys Glu Ile His Tyr Arg Gly Val Arg 20 25 30 Lys Arg Pro Trp Gly Arg Tyr Ala Ala Glu Ile Arg Asp Pro Gly Lys 35 40 45 Lys Ser Arg Val Trp Leu Gly Thr Phe Asp Thr Ala Val Glu Ala Ala 50 55 60 Arg Ala Tyr Asp Lys Ala Ala Arg Glu Tyr Arg Gly Ala Lys Ala Lys 65 70 75 80 Thr Asn Phe Pro Ile Ala Glu Lys Val Val Asp Tyr Asp Asp Glu Lys 85 90 95 Gln Ser Ser Ser Gln Ser Ser Thr Val Glu Ser Ser Ser Ser Pro Val 100 105 110 Val Ser Ala Val Ala Arg Asp Val Thr Arg Gln Val Gly Gly Val Val 115 120 125 Gly Met Gly Arg Phe Pro Phe Val Phe Gln Gln Gln Pro Pro His Val 130 135 140 Asn Ala Val Gly Pro Val Trp Phe Leu Asp Ser Thr Val Lys Pro Glu 145 150 155 160 Phe Val Ala Gln Arg Phe Pro Val Arg Tyr Asp Pro Val Gly Leu Glu 165 170 175 Gly Gly Ala His Ser Asp Ser Asp Ser Ser Ser Val Ile Asp Phe Lys 180 185 190 Pro Arg Ser Ser Ile Leu His Leu Asp Leu Asn Leu Pro Pro Pro Ala 195 200 205 Asp Ala 210 125243PRTPopulus trichocarpa 125Met Cys Thr Arg Gly His Trp Arg Pro Ala Glu Asp Glu Lys Leu Lys 1 5 10 15 Glu Leu Val Glu Lys Tyr Gly Pro His Asn Trp Asn Ala Ile Ala Glu 20 25 30 Lys Leu Gln Gly Arg Ser Gly Lys Ser Cys Arg Leu Arg Trp Phe Asn 35 40 45 Gln Leu Asp Pro Arg Ile Asn Arg Ser Pro Phe Thr Glu Glu Glu Glu 50 55 60 Glu Arg Leu Leu Ala Ser His Arg Ile His Gly Asn Arg Trp Ala Ile 65 70 75 80 Ile Ala Arg Phe Phe Pro Gly Arg Thr Asp Asn Ala Val Lys Asn His 85 90 95 Trp His Val Ile Met Ala Arg Arg Tyr Arg Glu Arg Ser Arg Leu His 100 105 110 Ala Lys Arg Ala Ala Gln Thr Leu Val Asn Asp Asn Lys Leu Ser Ser 115 120 125 Lys Gln Asp His Met His Met Asp Cys Glu Thr Arg Asn Phe Ser Ser 130 135 140 Phe Ser Lys Lys Tyr Cys Glu Lys Tyr Gly Gln Tyr Pro Met Val Thr 145 150 155 160 His Ser Tyr Leu Pro Ala Phe Cys Lys Glu Phe Tyr Asn Glu Asp Pro 165 170 175 Ser His Cys Glu Asp Gln Ser Arg Pro Ile Glu Phe Tyr Asp Phe Leu 180 185 190 Gln Val Asn Thr Asp Ser Asn Lys Ser Glu Val Ile Asp Asn Ala Arg 195 200 205 Arg Asp Asp Glu Glu Val Asp Gln Gln Glu Ala Leu Glu Asn Asn Gln 210 215 220 Ser Lys Ala Asp Val Pro Phe Ile Asp Phe Phe Ser Val Asn Gly Lys 225 230 235 240 Ser Ser Ser 126318PRTPopulus trichocarpa 126Met Gly Arg Ser Pro Cys Cys Glu Lys Glu His Thr Asn Lys Gly Ala 1 5 10 15 Trp Thr Lys Glu Glu Asp Glu Arg Leu Ile Asn Tyr Ile Lys Ser His 20 25 30 Gly Glu Gly Cys Trp Arg Ser Leu Pro Lys Ala Ala Gly Leu Leu Arg 35 40 45 Cys Gly Lys Ser Cys Arg Leu Arg Trp Ile Asn Tyr Leu Arg Pro Asp 50 55 60 Leu Lys Arg Gly Asn Phe Ser Asp Glu Glu Asp Glu Leu Ile Ile Asn 65 70 75 80 Leu His Ser Leu Leu Gly Asn Lys Trp Ser Leu Ile Ala Ala Arg Leu 85 90 95 Pro Gly Arg Thr Asp Asn Glu Ile Lys Asn Tyr Trp Asn Thr His Ile 100 105 110 Lys Arg Lys Leu Phe Ser Arg Gly Val Asp Pro Gln Thr His Arg Pro 115 120 125 Leu Asn Ser Thr Thr Thr Ser Ser Thr Thr Ser Thr Thr Thr Asn Ser 130 135 140 Thr Asn Asn Lys Asn Ser Asn Met Gly Thr Lys Arg Ile Thr Asn Phe 145 150 155 160 Lys Leu Glu Glu Gln Asn Tyr Leu Phe Val Gln Ala Gln Pro Glu Phe 165 170 175 Met Met Ser Asn Ile Val Lys Lys Ala Ser Asp Ser Ser Ile Ile Lys 180 185 190 Val Gly Gly Ser Ser Asp Ser Ala Glu Asp Ser Asn Ser Ser Ser Gly 195 200 205 Val Thr Ala Glu Leu Glu Val His Pro Asn His Lys Leu Asn Leu Glu 210 215 220 Leu Ser Ile Gly Leu Pro Cys Gln Ser Gln Leu Ser Ser Val Asn Asp 225 230 235 240 Leu Asn Asp Ser Lys Gln Ala Asn Gln Gln His Gln Glu Gln Val Val 245 250 255 Thr Tyr Gln Leu Phe Gly Thr Pro Ala Thr Pro Thr Ser Ser Ala Pro 260 265 270 Ala Val Val His Arg Thr Ala Cys Leu Cys Ser Tyr Asn Arg Gly Phe 275 280 285 Lys Asn Ser Gln Ala Cys Ser Cys Cys Asn Ala Val Glu Lys Phe Val 290 295 300 Thr Ala Asp Ser Leu Tyr Arg Phe Tyr Arg Pro Leu Asp Ala 305 310 315 127235PRTPopulus trichocarpa 127Met Glu Val Tyr Pro Tyr Pro Ser Arg Phe Ser Met Ser Ser Leu Cys 1 5 10 15 Ser Phe Gly Asn Phe Val Asp Lys Val Lys Glu Val Cys Asn Phe Val 20 25 30 Val Ser Ala Ile Ile Gly Asn Ile Phe Ser Ala Ile Phe Thr Phe Phe 35 40 45 Phe Ala Leu Val Gly Thr Leu Leu Gly Ala Met Thr Gly Ala Leu Ile 50 55 60 Gly Gln Glu Thr Glu Ser Gly Phe Val Arg Gly Ala Ala Val Gly Ala 65 70 75 80 Ile Ser Gly Ala Val Phe Ser Ile Glu Val Phe Glu Ser Ser Leu Val 85 90 95 Leu Trp Gln Ser Asp Glu Ser Gly Ile Gly Cys Val Leu Tyr Leu Ile 100 105 110 Asp Val Ile Ala Ser Leu Leu Ser Gly Arg Leu Val Arg Glu Arg Ile 115 120 125 Gly Pro Ala Met Leu Ser Ala Val Gln Ser Gln Met Gly Ala Val Glu 130 135 140 Thr Asn Phe Glu Glu Ile Pro Asn Ile Phe Asp Thr Gly Gly Ser Lys 145 150 155 160 Gly Leu Pro Gly Asp Ser Leu Glu Lys Ile Pro Lys Ile Arg Ile Thr 165 170 175 Ser Asn Asn Asn Val Asp Glu Ser Gly Glu Lys Val Ser Cys Ser Val 180 185 190 Cys Leu Gln Asp Phe Gln Leu Gly Glu Thr Val Arg Ser Leu Pro His 195 200 205 Cys His His Met Phe His Leu Pro Cys Ile Asp Lys Trp Leu Leu Arg 210 215 220 His Ala Ser Cys Pro Leu Cys Arg Arg Asp Leu 225 230 235 128293PRTPopulus trichocarpa 128Met Arg Ile Ser Arg Ala Arg Trp Phe Thr Phe Leu Arg Arg Val Phe 1 5 10 15 His Tyr Gln Asn Gly Ser Ser Ser Asn Leu Gly Ser Asn Pro Phe Asn 20 25 30 Ser Ser Ser Trp Met Met Leu Glu Phe Val Ala Leu Leu Leu Gln Ile 35 40 45 Cys Ile Thr Thr Phe Thr Leu Ala Ile Ser Lys Ala Glu Asn Pro Val 50 55 60 Trp Pro Val Arg Ile Trp Ile Ile Gly Tyr Asn Ile Gly Cys Val Leu 65 70 75 80 Ser Leu Leu Leu Leu Tyr Gly Arg Tyr Arg Gln Leu Asn Ala Thr Gln 85 90 95 Gly Asp Gly Phe Gly Leu Pro Asp Leu Glu Gln Gln Gly Gly Ser Glu 100 105 110 Glu Ser Ser Val Cys Arg Tyr Ser His Leu Met Asn Lys Cys Arg Thr 115 120 125 Ser Leu Glu Leu Phe Phe Ala Ile Trp Phe Val Met Gly Asn Val Trp 130 135 140 Val Phe Asp Ser Arg Phe Gly Ser Tyr Phe Arg Ala Pro Lys Leu His 145 150 155 160 Val Leu Cys Ile Ser Leu Leu Ala Trp Asn Ala Leu Ser Tyr Ser Phe 165 170 175 Pro Phe Leu Leu Phe Leu Leu Leu Cys Cys Cys Val Pro Leu Ile Ser 180 185 190 Thr Val Ile Gly Tyr Asn Met Ser Met Gly Ser Ala Glu Arg Gly Ala 195 200 205 Ser Asp Asp Gln Ile Ser Arg Leu Pro Ser Arg Arg Tyr Lys Ala Val 210 215 220 Asp Thr Asp Ser Glu Phe Arg Asn Ser Val Asp Cys Asp Ser Thr Val 225 230 235 240 Ala Ser Glu Asp Leu Glu Cys Cys Ile Cys Leu Ala Lys Tyr Lys Asp 245 250 255 Ile Glu Glu Val Arg Gln Leu Pro Cys Ser His Met Phe His Leu Lys 260 265 270 Cys Val Asp Gln Trp Leu Arg Ile Ile Ser Cys Cys Pro Leu Cys Lys 275 280 285 Gln Glu Leu Glu Lys 290 129321PRTPopulus trichocarpa 129Met Asp Pro Lys Gly Ser Asn Ser Lys Asn Pro His Glu Leu Pro Thr 1 5 10 15 Phe Phe Thr Leu Thr His Thr His Thr Ser Pro Ser Pro Ser Pro His 20

25 30 Thr Pro Pro Gln Pro His His Gln Gln Pro Gln His Leu His Asn Gln 35 40 45 Asn Gln Leu Gln Pro Asn Met Gly Glu Asn Lys Ala Ala Glu Ile Lys 50 55 60 Asp Phe Gln Ile Val Val Ala Asp Lys Glu Glu Gln Lys Lys Gln Leu 65 70 75 80 Ala Pro Lys Arg Ser Ser Asn Lys Asp Arg His Thr Lys Val Glu Gly 85 90 95 Arg Gly Arg Arg Ile Arg Met Pro Ala Leu Cys Ala Ala Arg Ile Phe 100 105 110 Gln Leu Thr Arg Glu Leu Gly His Lys Ser Asp Gly Glu Thr Ile Gln 115 120 125 Trp Leu Leu Gln Gln Ala Glu Pro Ser Ile Ile Ala Ala Thr Gly Thr 130 135 140 Gly Thr Ile Pro Ala Ser Ala Leu Ala Ala Ala Gly Gly Ala Ile Ser 145 150 155 160 Gln Gln Gly Ala Ser Leu Ser Ala Gly Leu His Gln Lys Ile Asp Asp 165 170 175 Ile Gly Glu Ser Ser Ser Arg Arg Thr Ser Trp Ala Met Leu Gly Gly 180 185 190 Asn Leu Gly Arg Pro His Gln Val Thr Ser Ala Gly Leu Trp Pro Pro 195 200 205 Val Gly Gly Tyr Gly Phe Gln Ser Ser Ser Asn Thr Thr Gly Pro Ser 210 215 220 Thr Thr Asn Ile Val Ser Glu Gly Gly Gly Gly Ser Ser Tyr Leu Gln 225 230 235 240 Lys Asn Gly Phe Ser Gly Phe Asp Met Pro Gly Asn Asn Ile Gly Pro 245 250 255 Met Ser Leu Thr Ser Ile Leu Gly Val Gly Ser Gln Gln Leu Pro Gly 260 265 270 Leu Glu Leu Gly Leu Ser Gln Asp Gly His Ile Gly Val Leu Ser Pro 275 280 285 Gln Ala Leu Ser Gln Ile Tyr Gln Gln Met Gly Gln Ala Arg Val Gln 290 295 300 Gln His Gln Gln Gln Asn Pro Ser Lys Asp Asp Ser Gln Gly Ser Gly 305 310 315 320 Gln 130692PRTPopulus trichocarpa 130Met Gly Glu Glu Ala Asp Pro Thr Met Lys Lys Lys Lys Lys Lys Gly 1 5 10 15 Arg Pro Ser Leu Leu Glu Leu Gln Lys Arg Ser Leu Lys Gln Gln Gln 20 25 30 Leu Gln Gln Thr Thr Pro Ile Ser Leu Lys Asn Pro Asn Pro Leu Asn 35 40 45 Ser Asn Ser Ala Leu Pro Asn Arg Arg Ser Ala Arg Arg Ser Ser Asn 50 55 60 Ser Tyr Ala Pro Glu Trp Ile Asp Gly Asp Asp Asp Glu Asp Asp Asp 65 70 75 80 Glu Asp Asp Glu Arg Lys Glu Lys Lys His Lys Leu Leu Arg Gly Leu 85 90 95 Asn Ser Gln Lys Asn Asn Asn Gln Asn Ser Asn Ser Ser Ser Pro Ser 100 105 110 Asn Leu His Gly Ser Asp Ser Asn Ala Gly Gly Gly Asn Gln Glu Asp 115 120 125 Gly Ile Arg Arg Arg Lys Ile Ser Ala Val Arg Leu Gly Ser Asp Asp 130 135 140 Leu Gly Glu Lys Val Leu Lys Gly Thr Asp Thr Ile His Gly Ser Ser 145 150 155 160 Val Glu Pro Gly Pro Thr Thr Pro Leu Pro Asp Lys Lys Leu Leu Val 165 170 175 Phe Ile Leu Asp Arg Leu Gln Lys Lys Asp Thr Tyr Gly Val Phe Ser 180 185 190 Glu Pro Val Asp Pro Glu Glu Leu Pro Asp Tyr Phe Asp Ile Val Glu 195 200 205 Asn Pro Met Asp Phe Ser Thr Val Arg Lys Lys Leu Asp Glu Gly Ala 210 215 220 Tyr Ala His Leu Glu Gln Phe Glu Lys Asp Val Leu Leu Ile Cys Ser 225 230 235 240 Asn Ala Met Gln Tyr Asn Pro Ser Asp Thr Ile Tyr Phe Arg Gln Ala 245 250 255 Arg Ala Met Gln Glu Leu Ala Lys Lys Asp Phe Glu Asn Leu Arg Gln 260 265 270 Asp Ser Asp Asp Ser Glu Pro Gln Thr Lys Val Ala Arg Arg Gly Arg 275 280 285 Pro Pro Ala Leu Gly Lys Leu Lys Lys Ala Leu Glu Arg Ser Pro Ile 290 295 300 Asp Arg Val Gly Pro Glu Ala Ser Ser Asp Ala Thr Leu Ala Thr Gly 305 310 315 320 Gly Asp His Asn Asn Leu Ser Asn Gly Tyr Asn Leu Arg Lys Ser Ser 325 330 335 Ser Tyr Lys Tyr Gln Pro Gly Asp Ala Phe Val Arg Ala Ser Tyr Ser 340 345 350 Ser Glu Asn Tyr Ser Thr Trp Leu Ser Glu Trp Glu Asn Glu Phe Pro 355 360 365 Ala Ser Val Val Lys Ala Val Met Lys Tyr Gly Lys Lys Pro Phe Val 370 375 380 Leu Asp Glu Asn Lys Arg Asp Thr Tyr Lys His Pro Leu Gly Ser His 385 390 395 400 Glu Pro Ser Ile Leu Ser Thr Phe Glu Gly Glu Leu Lys Gln Leu Val 405 410 415 Val Val Gly Leu Ser Ser Glu His Gly Tyr Ala Arg Ser Leu Ala Arg 420 425 430 Phe Ala Ala Asp Leu Gly Pro Val Val Trp Arg Ile Ala Ser Lys Lys 435 440 445 Ile Glu Ser Val Leu Pro Thr Gly Leu Glu Phe Gly Pro Gly Trp Val 450 455 460 Gly Glu Asn Lys Ala Met Glu Lys Gln Lys Ile Leu Asn Asn Leu Val 465 470 475 480 Ser Asp Asn His Leu Ser Arg Phe Gln Pro Ala Ala Ser Ser Ser Arg 485 490 495 Glu Ala Ala Trp Asn Arg Glu Gly Leu Pro Glu Thr Val Gly Gly Leu 500 505 510 Asn Pro Gln Asn Glu Leu Ala Thr Leu Asn Ser Gly Ala Gly Gly Met 515 520 525 Lys Ser Met Pro Ser Leu Gln Ile Gln Gln Lys Pro Ile Ile His Pro 530 535 540 Asp Met Asn Gly Phe Ser Gly Gly Phe Gly Tyr Asn Ser Ser Pro Gln 545 550 555 560 Pro Gly Met Ala Arg Thr Val Ala Pro Thr Gly Lys Leu Asn Leu Glu 565 570 575 Gln Thr Ala Val Pro Ser Gln Met Phe Gly Val Val Pro Thr Gly Asn 580 585 590 Ser Ala Phe Ile Ser Val Pro Gly Asn Asp Phe Asn Thr Asn Lys Gly 595 600 605 Met Leu Ser Glu Thr Ser Ser Gly Leu Leu Gln Pro Gly Ile Ser Pro 610 615 620 Ala Val Gly Ser Ser Ser Asp Ser Arg Thr Phe Gly Asn Val Gly Phe 625 630 635 640 Gly Gly Lys Ser Ser Trp Gln Gly Phe Leu Pro Tyr Gln Gln Gln Gly 645 650 655 Thr Val Pro Phe Pro Pro Asp Leu Asn Val Gly Phe Leu Ala Pro Gly 660 665 670 Ser Pro Thr Ser Ser Val Pro Ile Gly Ser Pro Arg Gln Pro Asp Leu 675 680 685 Ala Leu Gln Leu 690 1311113PRTPopulus trichocarpa 131Met Lys Ser Pro Ala Asn Gly Ala Ala Ala Ala Val Thr Asn Gly Glu 1 5 10 15 Gly Val Glu Lys Lys Ser Ile Asn Pro Glu Leu Trp Gln Ala Cys Ala 20 25 30 Gly Pro Leu Val Asn Leu Pro Ala Ala Gly Thr His Val Val Tyr Phe 35 40 45 Pro Gln Gly His Ser Glu Gln Val Ala Ala Ser Leu Lys Lys Asp Val 50 55 60 Asn Ala Gln Ile Pro Asn Tyr Pro Asn Leu Pro Ser Lys Leu Leu Cys 65 70 75 80 Leu Leu His Asn Val Thr Leu His Ala Asp Pro Glu Thr Asp Glu Val 85 90 95 Tyr Val Gln Met Thr Leu Gln Pro Val Ser Ser Phe Asp Lys Asp Ala 100 105 110 Leu Leu Arg Ser Asp Leu Ala Leu Lys Ser Asn Lys Pro Gln Thr Glu 115 120 125 Phe Phe Cys Lys Thr Leu Thr Ala Ser Asp Thr Ser Thr His Gly Gly 130 135 140 Phe Ser Val Pro Arg Arg Ala Ala Glu Lys Thr Phe Pro Pro Leu Asp 145 150 155 160 Phe Ser Met Gln Pro Pro Ala Gln Glu Leu Val Ala Arg Asp Leu His 165 170 175 Asp Asn Val Trp Thr Phe Arg His Ile Tyr Arg Gly Gln Pro Lys Arg 180 185 190 His Leu Leu Thr Thr Gly Trp Ser Leu Phe Val Ser Gly Lys Arg Leu 195 200 205 Phe Ala Gly Asp Ser Val Leu Phe Met Arg Asp Glu Lys Gln Gln Leu 210 215 220 Leu Leu Gly Ile Arg Arg Ala Asn Arg Gln Pro Thr Asn Leu Ser Ser 225 230 235 240 Ser Val Leu Ser Ser Asp Ser Met His Ile Gly Ile Leu Ala Ala Ala 245 250 255 Ala His Ala Ala Ala Asn Asn Ser Pro Phe Thr Val Tyr Tyr Asn Pro 260 265 270 Arg Ala Ser Pro Ser Glu Phe Val Ile Pro Leu Ala Lys Tyr Tyr Lys 275 280 285 Ala Val Tyr Ser Asn Gln Ile Ser Leu Gly Met Arg Phe Arg Met Met 290 295 300 Phe Glu Thr Glu Glu Ser Gly Thr Arg Arg His Met Gly Thr Ile Thr 305 310 315 320 Gly Ile Ser Asp Leu Asp Ala Val Arg Trp Lys Asn Ser Gln Trp Arg 325 330 335 Asn Leu Gln Val Gly Trp Asp Glu Ser Thr Ala Gly Glu Arg Arg Asn 340 345 350 Arg Val Ser Ile Trp Glu Ile Glu Pro Val Thr Ala Pro Phe Phe Ile 355 360 365 Cys Pro Pro Pro Phe Phe Arg Ser Lys His Pro Arg Gln Pro Gly Met 370 375 380 Pro Asp Asp Asp Ser Thr Asp Phe Asp Ser Leu Phe Lys Arg Thr Met 385 390 395 400 Pro Trp Leu Gly Asp Asp Ile Tyr Met Lys Asp Pro Gln Val Leu Pro 405 410 415 Gly Leu Ser Leu Ala Gln Arg Met Asn Met Gln Gln Asn Pro Ser Leu 420 425 430 Ala Asn Ser Met Gln Pro Asn Tyr Met Gln Ser Leu Ser Gly Ser Val 435 440 445 Leu Gln Asn Leu Pro Gly Gly Asp Leu Ser Arg Gln Leu Gly Leu Ser 450 455 460 Ser Pro Gln Met Pro Gln Pro Asn Asn Leu Gln Phe Asn Ala Gln Arg 465 470 475 480 Leu Pro Gln Gln Ala Gln Gln Leu Asp Gln Leu Pro Lys Leu Gln Ser 485 490 495 Leu Leu Asn Pro Leu Gly Ser Ile Ile Gln Ser Gln Gln Gln Met Gly 500 505 510 Asp Ile Thr Gln Gln Ser Arg Gln Asn Met Met Ala Gln Thr Leu Pro 515 520 525 Ser Ser Gln Val Gln Ala Gln Leu Leu Gln Pro Gln Thr Leu Ala Gln 530 535 540 Thr Asn Asn Ile Leu Gln Gln Gln Pro Ser Ile Gln Ser His Gln Leu 545 550 555 560 Leu Arg Asn Leu Pro Gln Thr Leu His Gln Gln Gln Gln Asn Gln Gln 565 570 575 Gln His Ile Met Gly Gln Asn Gln Gln Gln Ser Leu Met Gln Ser Gln 580 585 590 Leu Ser Asp Gln Val Asn Gln His Met Gln Met Ser Asp Asn Gln Ile 595 600 605 Gln Ser Gln Leu Met Gln Lys Leu Gln Gln Gln Gln Gln Ser Val Ser 610 615 620 Ala Gln Gln Ser Ala Met His Gln Ala Gly Gln Leu Gly Gln Leu Gln 625 630 635 640 Asp Ser Gln Arg Gln Leu Leu Asp Ala Ser Gln Ser Phe Ser Arg Ser 645 650 655 Met Thr Pro Ser Gln Met Leu Glu Ile Pro Gln Thr Thr Pro Thr Ser 660 665 670 Leu Pro Gln Pro Asn Thr Ile Pro Gln Gln Met Thr Lys Asn Asn Asn 675 680 685 Gln Thr Asn Thr Arg Phe Ser His Leu Pro Gln Gln Leu Lys Pro Gln 690 695 700 Gln Gln His Ser Gly Ile Met Leu Leu Ser Glu Met Ala Gly His Met 705 710 715 720 Gly Leu Pro Pro Ser Ser Met Ala Asn Gln Leu Ser Thr Ala Gly Ser 725 730 735 Ser Ile Leu Thr Ala Ala Ala Gly Pro Gly Gln Ser Gly Ile Thr Asp 740 745 750 Asp Val Pro Ser Cys Ser Thr Ser Pro Ser Thr Asn Asn Cys Pro Asn 755 760 765 Ile Val Gln Pro Met Ile Asn Gly Trp Ala His Arg Ser Thr Ala Met 770 775 780 Gly Glu Asp Met Ala Gln Ser Ala Val Thr Leu Phe Ser Pro Ser Ala 785 790 795 800 Leu Glu Thr Val Ser Ser Asn Gly Asn Leu Val Lys Asp Leu Leu Gln 805 810 815 Lys Ser Glu Val Lys Pro Ser Leu Asn Ile Ser Lys Asn Gln Asn Pro 820 825 830 Gly Leu Phe Ser Ser Gln Thr Tyr Leu Asn Gly Val Ala Ala Gln Ile 835 840 845 Asp Tyr Leu Asp Thr Ser Ser Ser Thr Thr Ser Val Cys Leu Ser Gln 850 855 860 Asn Asp Val His Leu Gln Gln Asn Asn Asn Ser Leu Ser Tyr Asn Pro 865 870 875 880 Gln Ser Val Leu Leu Arg Asp Ala Ser His Asp Gly Glu Leu Gln Gly 885 890 895 Asp Pro Arg Asn Asn Ile Leu Tyr Gly Thr Asn Ile Asp Ser Gln Leu 900 905 910 Val Met Pro Ile Asn Ser Asp His Leu Leu Thr Lys Gly Met Met Gly 915 920 925 Leu Gly Lys Asp Phe Ser Asn Asn Phe Ser Ser Gly Gly Met Leu Thr 930 935 940 Asn Cys Glu Asn Ser Lys Asp Pro Gln Gln Glu Leu Ser Ser Ala Ile 945 950 955 960 Val Ser Lys Ser Phe Gly Val Pro Asp Met Pro Phe Asn Ser Ile Asp 965 970 975 Ser Thr Ile Asn Asp Ser Ser Leu Leu Asn Arg Gly Ser Trp Ala Pro 980 985 990 Pro Gln Gln Gln Phe Gln Arg Met Arg Thr Tyr Thr Lys Val Tyr Lys 995 1000 1005 Arg Gly Ala Val Gly Arg Ser Ile Asp Ile Thr Arg Tyr Ser Gly 1010 1015 1020 Tyr Asp Glu Leu Lys Gln Asp Leu Ala Arg Arg Phe Gly Ile Glu 1025 1030 1035 Gly Gln Leu Glu Asp Gln Gln Arg Ile Gly Trp Lys Leu Val Tyr 1040 1045 1050 Thr Asp His Glu Asn Asp Val Leu Leu Val Gly Asp Asp Pro Trp 1055 1060 1065 Glu Glu Phe Val Asn Cys Val Arg Cys Ile Lys Ile Leu Ser Pro 1070 1075 1080 Gln Glu Val Gln Gln Met Ser Leu Asp Gly Asp Phe Gly Asn Ser 1085 1090 1095 Val Leu Pro Asn Gln Ala Gly Ser Ser Ser Asp Asn Val Asn Ala 1100 1105 1110 132501PRTPopulus trichocarpa 132Met Val Gly Gly Glu Asn Leu Ser Arg Ile Thr Val Ser Lys Ala Ser 1 5 10 15 Gly Glu Asp Thr Ile Ser Asn Val Ser Ser Phe Gly Asn Gln Gly Met 20 25 30 Pro His Ser Ile Thr Val Pro Pro Pro Lys Lys Lys Arg Asn Leu Pro 35 40 45 Gly Met Pro Asp Pro Asp Ala Glu Val Val Ala Leu Ser Pro Lys Thr 50 55 60 Leu Val Ala Thr Ile Arg Phe Val Cys Glu Ile Cys Asn Lys Gly Phe 65 70 75 80 Gln Arg Asp Gln Asn Leu Gln Leu His Arg Arg Gly His Asn Leu Pro 85 90 95 Trp Lys Leu Lys Gln Arg Thr Asn Lys Glu Pro Arg Lys Arg Val Tyr 100 105 110 Val Cys Pro Glu Ser Ser Cys Val His His Asn Pro Ala Arg Ala Leu 115 120 125 Gly Asp Leu Thr Gly Ile Lys Lys His Phe Tyr Arg Lys His Gly Glu 130 135 140 Lys Lys Trp Lys Cys Glu Arg Cys Ser Lys Lys Tyr Ala Val Gln Ser 145 150 155 160 Asp Trp Lys Ala His Leu Lys Thr Cys Gly Thr Lys Glu Tyr Lys Cys 165 170 175 Asp Cys Gly Thr Leu Phe Ser Arg Arg Asp Ser Phe Val Thr His Arg 180 185

190 Ala Phe Cys Asp Ala Leu Ala Glu Glu Ser Ala Arg Ala Gln Thr Leu 195 200 205 Ala Ile Met Gly Arg Glu Gly Asn Gly Cys Asp Ile Lys Arg Val Gly 210 215 220 Ala Ser Pro Pro Pro Pro Pro Leu Thr Pro Ser Thr Ser Val Val Ser 225 230 235 240 Pro Gly Leu Ser Val Gln Ser Ser Glu Leu Ala Glu Asn Pro Ile Gly 245 250 255 Leu Ser Pro Pro Pro Ile Ala Cys Ala Ser Ala Thr Ser Thr Ser Ser 260 265 270 Thr Ser Ser Thr Ser Asn Val Phe Ala Thr Thr Phe Ala Ser Ser Thr 275 280 285 Ala Thr Pro Ala Ala Ile Pro Gln Gln Ala Ser Val Pro Ser Ser Phe 290 295 300 Pro Asn Leu Phe Cys Gly Leu Ala Arg Ser Asp Tyr Pro Thr Thr Met 305 310 315 320 Pro Thr Pro Arg Ala Ile Glu Pro Pro Ser Leu Ser Leu Ser Pro Ser 325 330 335 Phe Tyr Leu Ser Asn Asn Thr Ser Ser Leu Phe Ser Thr Glu Gln Glu 340 345 350 His Tyr His Tyr Thr Pro Ser Pro Gln Pro Ala Met Ser Ala Thr Ala 355 360 365 Leu Leu Gln Lys Ala Ala Gln Met Gly Ala Thr Thr Ser Asn Pro Ser 370 375 380 Phe Leu Arg Gly Leu Gly Leu Pro Arg Ser Thr Asn Gln Asp Ser Asn 385 390 395 400 Cys Asn Lys Trp Asp Val Lys Pro Glu Asn Asn Thr Thr Val Ala Ala 405 410 415 Gly Leu Gly Leu Gly Leu Pro Ser Ser Asp Val Met Met Gly Ser Ser 420 425 430 Ser Leu Phe Gly Asn Lys Pro Ala Thr Leu Asp Leu Leu Gly Leu Gly 435 440 445 Met Asp Ala Ala Ser Ser Ala Leu Leu Asn Ser Tyr Ser Gly Gly Phe 450 455 460 Asn Val Gly Ala Ala Thr Ala Ala Ala Tyr Gly Gly Gly Gly Gly Arg 465 470 475 480 Gly Thr Ser Glu Glu Thr Trp Asp Gly Val Pro Glu Arg Lys Pro Tyr 485 490 495 Gly Ser Thr Gly Ala 500 133321PRTPopulus trichocarpa 133Met Asp Pro Lys Gly Ser Asn Ser Lys Asn Pro His Glu Leu Pro Thr 1 5 10 15 Phe Phe Thr Leu Thr His Thr His Thr Ser Pro Ser Pro Ser Pro His 20 25 30 Thr Pro Pro Gln Pro His His Gln Gln Pro Gln His Leu His Asn Gln 35 40 45 Asn Gln Leu Gln Pro Asn Met Gly Glu Asn Lys Ala Ala Glu Ile Lys 50 55 60 Asp Phe Gln Ile Val Val Ala Asp Lys Glu Glu Gln Lys Lys Gln Leu 65 70 75 80 Ala Pro Lys Arg Ser Ser Asn Lys Asp Arg His Thr Lys Val Glu Gly 85 90 95 Arg Gly Arg Arg Ile Arg Met Pro Ala Leu Cys Ala Ala Arg Ile Phe 100 105 110 Gln Leu Thr Arg Glu Leu Gly His Lys Ser Asp Gly Glu Thr Ile Gln 115 120 125 Trp Leu Leu Gln Gln Ala Glu Pro Ser Ile Ile Ala Ala Thr Gly Thr 130 135 140 Gly Thr Ile Pro Ala Ser Ala Leu Ala Ala Ala Gly Gly Ala Ile Ser 145 150 155 160 Gln Gln Gly Ala Ser Leu Ser Ala Gly Leu His Gln Lys Ile Asp Asp 165 170 175 Ile Gly Glu Ser Ser Ser Arg Arg Thr Ser Trp Ala Met Leu Gly Gly 180 185 190 Asn Leu Gly Arg Pro His Gln Val Thr Ser Ala Gly Leu Trp Pro Pro 195 200 205 Val Gly Gly Tyr Gly Phe Gln Ser Ser Ser Asn Thr Thr Gly Pro Ser 210 215 220 Thr Thr Asn Ile Val Ser Glu Gly Gly Gly Gly Ser Ser Tyr Leu Gln 225 230 235 240 Lys Asn Gly Phe Ser Gly Phe Asp Met Pro Gly Asn Asn Ile Gly Pro 245 250 255 Met Ser Leu Thr Ser Ile Leu Gly Val Gly Ser Gln Gln Leu Pro Gly 260 265 270 Leu Glu Leu Gly Leu Ser Gln Asp Gly His Ile Gly Val Leu Ser Pro 275 280 285 Gln Ala Leu Ser Gln Ile Tyr Gln Gln Met Gly Gln Ala Arg Val Gln 290 295 300 Gln His Gln Gln Gln Asn Pro Ser Lys Asp Asp Ser Gln Gly Ser Gly 305 310 315 320 Gln 134229PRTPopulus trichocarpa 134Met Glu Pro Ser Ser Ser Ser Ala Val Ala Ala Ala Ile Val Ser Thr 1 5 10 15 Asn Ala Asn Ile Asn Thr Asp Arg Thr Arg Arg Lys Lys Lys Lys Lys 20 25 30 Ser Val Leu Gln Gln His Gln Ser Lys Gln Asn Gln Asn Ser Gln Ser 35 40 45 His Ala Lys Trp Lys Thr Glu Ala Gln Gln Gln Val Tyr Ser Ser Lys 50 55 60 Leu Ile Gln Ala Leu Ser Gln Val Asn Leu Asn Pro Ser Thr Ser Ser 65 70 75 80 Ala Pro Arg Gln Gly Arg Ala Val Arg Glu Val Ala Asp Arg Ala Leu 85 90 95 Ala Phe Ala Ala Lys Gly Lys Thr Arg Trp Ser Arg Ala Ile Leu Thr 100 105 110 Ser Arg Ile Lys Leu Lys Phe Arg Lys Gln Gln His Lys Arg Gln Arg 115 120 125 Leu Ala Ser Ser Ser Ser Ser Ser Pro Gly Ser Thr Thr Gly Ser Ser 130 135 140 Ser Arg Ser Ser Arg Lys His Lys Val Ser Val Leu Arg Leu Lys Ala 145 150 155 160 Lys Gly Leu Pro Ala Val Gln Arg Lys Val Arg Val Leu Gly Arg Leu 165 170 175 Val Pro Gly Cys Leu Lys Gln Pro Leu Pro Val Ile Leu Glu Glu Ala 180 185 190 Thr Asp Tyr Ile Ala Ala Leu Glu Met Gln Val Lys Thr Met Thr Ala 195 200 205 Ile Ala Glu Leu Leu Ser Arg Ser Thr Ser Glu Ala Ser Ser Thr Ser 210 215 220 Glu Pro Met Thr Ser 225 13519DNAArtificialforward cloning primer 135atgcaaccag gcttggatg 1913619DNAArtificialForward cloning primer 136atggcgagag gaaaggttc 1913725DNAArtificialForward cloning primer 137atggcatata tgtgtgcaga tagcg 2513821DNAArtificialForward cloning primer 138atggggagag gtagggttga g 2113925DNAArtificialForward cloning primer 139atgtctttag ttggacctgc agaac 2514028DNAArtificialForward cloning primer 140atgaagagag atcatcaaga aaccattg 2814127DNAArtificialForward cloning primer 141atggaaaaca attgtgtgta tagtgag 2714222DNAArtificialForward cloning primer 142atgagttttg ggggtttcct tg 2214322DNAArtificialForward cloning primer 143atggcaccca agaagcttaa tg 2214426DNAArtificialForward cloning primer 144caccatgtgc actagaggac attgga 2614522DNAArtificialForward cloning primer 145atgggcagat ctccttgttg tg 2214625DNAArtificialForward cloning primer 146atggaggttt atccataccc atctc 2514727DNAArtificialForward cloning primer 147atgagaatat ctagagcaag atggttc 2714823DNAArtificialForward cloning primer 148atggatccca agggctctaa ctc 2314919DNAArtificialForward cloning primer 149atgggcgagg aagcagacc 1915019DNAArtificialForward cloning primer 150atgaagtcac cggccaacg 1915123DNAArtificialForward cloning primer 151atggtgggtg gtgagaattt atc 2315221DNAArtificialForward cloning primer 152atggatccca agggctctaa c 2115320DNAArtificialForward cloning primer 153atggaaccat cgtcgtcagc 2015422DNAArtificialReverse cloning primer 154taaatgcagt aaatgatgga gc 2215520DNAArtificialReverse cloning primer 155gcacgagaaa aactacaacc 2015624DNAArtificialReverse cloning primer 156ttatcgccat gacaaaactg tgag 2415723DNAArtificialReverse cloning primer 157cattacttgc actgttccta cgg 2315829DNAArtificialReverse cloning primer 158gtaatcaaat agtagctgtt acttccatg 2915923DNAArtificialReverse cloning primer 159gtcattgttg tgaatcacca gcg 2316028DNAArtificialReverse cloning primer 160aagttcagta attccaaagg taatcaag 2816124DNAArtificialReverse cloning primer 161gcataatcag ctttcacatt ggag 2416219DNAArtificialReverse cloning primer 162ccatcaagca tcggctggc 1916329DNAArtificialReverse cloning primer 163gttgtagtat ataatacaag actactgag 2916421DNAArtificialReverse cloning primer 164cctggtggtc atcaagcatc c 2116524DNAArtificialReverse cloning primer 165tcacagatcc cttctacaca gagg 2416629DNAArtificialReverse cloning primer 166ctatttttcc agttcttgtt tacagagag 2916724DNAArtificialReverse cloing primer 167ttactgccct gatccttgtg aatc 2416823DNAArtificialReverse cloning primer 168tcagagctgc aatgctaaat ctg 2316929DNAArtificialReerse cloning primer 169ttatgcattg acattatcag aactactgc 2917025DNAArtificialReverse cloning primer 170tcaagctcct gtagatccat aaggc 2517124DNAArtificialReverse cloning primer 171ttactgccct gatccttgtg aatc 2417223DNAArtificialReverse cloning primer 172ttaggaggtc atcggctcag aag 23173561DNAPopulus tremula x tremuloid 173atgcaaccag gcttggatga gatcacaatg acggggtgga agaatctgaa gcaacggctg 60tcattcaagg gcctgggtag ttgctgcggg agcacaagct ggatttccag aagtaccacc 120caaaccatgc cctttatcga tatagaggaa gaagaagagg aagagaccac catgcaaaac 180caagctcaaa gaggaggagg aggaggagca gcagcagcgc caggtgctgg gatgaatctg 240gcaatggcat tagctgctga gcgcgattta ggggattcaa atgtcaagac attgatgagt 300ttgatcgaag aaacggacgg tgttgattgg aggaagaaga ataacagtaa taacaaaagc 360aggagggaca aggaacagga acagaagcag gaagaagaga aggattgggt atgctgcgtg 420tgcatggaga gaaataaagg agcagctttt attccatgtg gacacacctt ttgtagggtt 480tgttcaagag aaatgtgggt taatcgaggg tgctgtcctg tctgcaaccg ttccattctc 540gacatccttg atatcttcta g 561174660DNAPopulus tremula x tremuloides 174atggcgagag gaaaggttca gctgaaaagg atagagaatg caactagcag gcaagtgacc 60ttctccaaga gaaaaaatgg gttattgaag aaagcttatg agctatcaat tctgtgcgat 120gctgaagttg cagtgatcat attttcacag aaaggaacac tctttaagtt tgcaagcact 180gatcagatac aaaagacgat tgaccggtac cgtaaaaatg caaagcaatt gcacactgac 240aggattgacg tggaacaata ttctaaggag caattaagac aagaatcaga aaacatggcc 300aagaagattg agataatcga gattttgcaa cgaaagcttt tagggcaaga tttagattca 360tgctctcccg aagagctcca tgacattggc aatcagcttg agatcagttt aagcaatatc 420agggctagaa agactcagtt attcaaggag cagatagaac agctgaaagc aaaggaaaga 480ttgttgttaa tggagaatgc aaggttgact aaacagcgcg atgcacagcc attgcagcaa 540tcaactcaat cgaaccaagt ggtgtcatac ttgagctcat gtagcaagag ttcagatatc 600gtggagactg atctgtacat tggacagcca cagatgcgct ggttgtagtt tttctcgtgc 660175916DNAPopulus tremula x tremuloides 175atggggagag gtagggttga gttgaagaga attgagaaca agatcaacag gcaagtaaca 60tttgcaaaga gaaggaatgg acttttgaag aaagcctatg agctttccgt tctttgtgat 120gcagaggttg ctctcatcat cttctccaat agaggaaagc tgtacgagtt ttgcagtagt 180tcaagcatgc tcaaaactct tgagaggtac cagaagtgca attatggagc accagagcca 240aatgtgtctg caagggaggc cctggaactg agtagtcagc aggaatatct gaagcttaaa 300gcacgttatg aagccctgca aagaacccaa aggaatcttt tgggagaaga acttggccct 360ctaagcagca aagagctaga atcacttgaa aggcagcttg atatgtcatt gaagcagatc 420agatcaacaa ggacccaata catgctggat cagctcaatg acctacagca taaggagcac 480atgctgactg cagctaataa gtccctgaaa gaaaggttga tggaaggcta ccaattaaat 540tcactccagt tgaatccaag tgcagaagat gtggagtacg ctagacaaca agcccaaccc 600cagggtgatg ggttttttca tgctttggag tgtgaaccta cactacaaat tgggtatcag 660ccagaaaata taacaatggt cactgctggc ccaagtatga ctacttacat gccaggttgg 720ttagcatgat aagaagacta gctatggttc tcactctcag ccatgcatgg tattcctttc 780ctcaacatga tggttcccat atttgatcag caaatttgtg ttttgtcttg tatgttattg 840atgaaacagt cttcgtttag cacgtaggtg gtctatatca taattctact gcaccgtagg 900aacagtgcaa gtaatg 916176636DNAPopulus tremula x tremuloides 176atggcaccca agaagcttaa tggtaatgat gacaatggtt ccttgaagaa agcaagtggt 60gatcatgata agaaggaaat ccattatagg ggagtgagga agaggccatg ggggaggtat 120gctgctgaga taagggatcc cgggaagaaa agccgggttt ggttgggcac gtttgacacg 180gcagtggagg ctgcaagggc ctatgataag gcggcgcgtg agtatcgtgg tgccaaggcg 240aaaaccaact ttccattagc ggagaaggtg gttgattatg acgatgagaa gcagagctct 300agccagagca gcaccgttga gtcgtcaagc tccccggtgg tttctgcggt ggcgcgtgat 360gtaactcgcc aggttggtgg ggttttgggg atggggaggt ttcccttcgt gttccagcag 420cagcagccga atgtaaacgc tgttggtcct gtctggtttc ttgatggtgt taagcctcag 480tttgtggctc agcgtttccc tgtacggttt gacccggtgg gtctcgaggg cggggcccac 540agtgactcgg attcatcatc tgtgattgat tttaagccaa ggagttcaat tcttgatttt 600gatcttaacc tgcctccgcc agccgatgct tgatgg 636177827DNAPopulus tremula x tremuloides 177atgtgcacta gaggacattg gaggcctgct gaggatgagg aacttaagga attggttgaa 60aagtatggtc ctcataattg gaacgccatt gccgaaaagc ttcaaggaag atcagggaag 120agttgtaggt tgagatggtt taatcagctg gatccaagaa tcaatagaag cccgtttaca 180gaagaggaag aagaaagact acttgcttcc cacaggattc atgggaatag atgggcaatt 240attgcaagat ttttccctgg tcgcaccgat aatgcagtga agaatcattg gcatgtcatc 300atggcaagaa gatatagaga gaggtctaga cttcatgcaa aaagggctgc tcaaactttg 360gtaaatgata acaaattatc ctcaaaacaa gatcacatgc acattgattg cgagacgagg 420aacttttctt catttgccaa gaaatattgt gaaaaatatg gccactatcc tatggttact 480cacagctact taccggcctt tggcaaagag ttctacaatg cagatccaag tcattgtgaa 540gatcaaagtc ggccgattga gttttatgat tttctccaag taaacactga ctccaacaaa 600agtgaagtga tagacaatgc aagaagagat gacgaggagg tagatcagca ggaagccatg 660gaaaatgatc agagcaaggg tgatgttcca ttcattgatt ttttctctgt taatggcaaa 720tcctcatcat aacacatgca gcttggtggt gtgtcaaacg agggaccaac aaatgcatag 780taagcataca actaattact cagtagtctt gtattatata ctacaac 827178957DNAPopulus tremula x tremuloides 178atggatccca agggctctaa ctcaaaaaac ccacatgagt tagccacttt cttgacccac 60acccaccctt ctcctcctca tcctcctcct cctccacaac cccatcttca acaaccacaa 120caactccata gccaaaacca acaacaaccc aacatgggag acaacaaacc agcagaaatc 180aaagactttc agattgtagt agctgacaaa gaagagcaaa agaaacagtt agcaccaaag 240agaagctcaa acaaagacag acacacaaaa gttgaaggta gaggtagaag gataaggatg 300ccagctcttt gtgcagcgag aatctttcaa ttgacaagag aattgggtca caaatctgat 360ggagagacaa tacagtggct tctacaacaa gctgaaccat ctataattgc agcaactggg 420actggtacta tacctgcatc agctttagca gctgctggcg gtgcaatttc acaacaagga 480gcttctcttt ctgctggttt gcatcaaaag atcgatgatt taggtgggtc cagtagtagt 540agggccagtt gggcaatgtt aggtggcaat ttagggagac cccatcatgt taccactgca 600ggattatggc ccccagttgg aggttatggg ttccagtcat catctagtac cactggtcca 660tcaacaacaa atatagtaag tgaaggtggt ggtggttcga gttatttgca aaaaaatgga 720ttttcagggt ttgacttgcc aggaaacaat attgggccta tgagttttac ttcaatttta 780ggtgtgggta gccagcagtt accaggattg gagcttgggt tgtcacaaga tgggcatatt 840ggggttttga gcccacaagc tttgagtcag atttatcagc aggtggggca ggctagggtg 900cagcagcacc agcagcaaaa tccttctaaa gatgattcac aaggatcagg gcagtaa 957179681DNAPopulus tremula x tremuloides 179atggaaccat cgtcgtcagc agcagcagca atagtgagca ctaacgccaa catcaacacg 60gatcgaacaa gaaggaaaaa gaagaagaaa tcagtgttgc agcaacacca atcaaaacag 120aaccaaaact cacagagcca cgccaaatgg aaaacagaag cacaacaaca agtctactca 180tccaaactca tccaagcctt aagccaagtc aatctcaacc cttcaacttc atcagccccg 240cgtaaaggtc gagccgttag agaagtggct gatcgagctt tagctttcgc tgctaaaggt 300aaaaccaggt ggagccgagc cattttaact agccgcatca aactcaaatt tcggaaacaa 360cataagagac agagacttgc gtcgtcgtcg tcttcttctc caggatccac taccgggagt 420ggcagccggt cgtcgaggaa gcataaagtg agtgttttga ggttgaaagc gaagggtttg 480ccggctgttc aaaggaaagt tcgtgttctt gggcggttag ttcctggttg ccggaaacaa 540ccattgcctg ttattttgga

agaagctaca gattatattg ctgctttgga gatgcaagtt 600aaaaccatga ctgctatagc tgagcttctt tctcgctcta cctccgaagc cagctctact 660tctgagccga tgacctccta a 681180447DNAPopulus tremula x tremuloides 180atggcatata tgtgtgcaga tagcggaaat ctaatggcaa tagcccaaca agttattaag 60caaaaacaac aacaagaaca acagcagcaa caaagccacc accaccacca taaccaacag 120caacaatttc ttggcctaaa cccattttct ttaaatcctt ggccgactaa tataatgtct 180gctaacccaa acttgggtta cgggctctcg ggtccggctg ctttctctga cccttttcag 240agtggacaag aaacaggtga cccacctgtg tttagtttct cgaacatgga gcagcaccac 300tcaagcgggt ttcggcttcc ttattttact ggagctggtg gtgagtttga ctcggatgag 360tggatggaca gtttaatgaa cggtggagat tcaacggata gttctaatct tccttctggt 420tgtgacgcgt ggcaaaacag tactgat 447181473DNAPopulus tremula x tremuloides 181atgtctttag ttggacctgc agaactctct gccagagcat acggaaataa taagctgtac 60tcactgaaag ggagcaatga caactctggt ttgtctgccc aaatattcgg gcctgataaa 120cggcagaaca tgtatatgac cgattcttac tcttgtgaga gttatgagaa gttcttcctt 180gattccccaa cagaagaaat aatacaacca tcaagttctg acatttcagg gaactcagct 240catccacaag gggcatcttc ctaccagcca agaaagagtt caggttcaac catgttccct 300caagatccct acaatgcttc tttcaatttg accacaccct gcgatggcta tccattcatt 360tccgagtcag attacttgga cattgagagc ccagatccac taaactatga tgaatataag 420atgaaattaa agcttcagga acttgagaga gcgcttctaa atgataatga tga 473182219DNAPopulus tremula x tremuloides 182atgaagagag atcatcaaga aaccattggt ggtggtattg gaaacagagg tgaatcttct 60tcttcatcaa tggaaactgg gaaaggaaaa tcatgggttg aagatgatca agatgcgggt 120ggcatggatg aattacttgc tgttttgggt tacaaaatca agtcttcaga tatggctgat 180gtagctcaaa agcttgaaca actaaagatg gctttgggt 219183320DNAPopulus tremula x tremuloides 183atggaaaaca attgtgtgta tagcgaggaa gatgagcaga tggaattgcc tccgggattt 60agattccatc caactgatga agagctcata actcactacc tgtctcagaa ggttcttgac 120aactacttct gtgctagagc tattggcgag gtcgatttaa acaagtgtga gccatgggat 180ttgccttgga gggctaaaat gggtgaaaag gaatggtatt tcttctgtgt tatagaccgg 240aagtatccca ccggtttaag gacgaacagg gctactgatg ctggttactg gaaagccaca 300ggcaaagaca aggaaattta 320184440DNAPopulus tremula x tremuloides 184atgagttttg ggggtttcct tgaaaacact agtcctggtg gtggtggcgc aagaattgtg 60gctgatatac cttataacat taacaacaac atgcccactg gtgcaatagc tcatactcgc 120cttgtctctc cttctctcac taaatccatg ttcaactctc ctggactctc tctagccctt 180ccaaacataa atggtcaagg agatataact aaaatggccg agaactttga gacaagtgtg 240ggtaggagaa gcaaagaaga ggagcatgag agcagatctg gtagtgataa catggatggt 300gcgtctggtg acgatcaaga tgcagctgaa aatcctccaa gaaaaaagag ataccaccga 360cacactccac aacaaatcca agaacttgaa gctttgttta aggaatgtcc tcatcctgac 420gagaaacaaa gattggagct 440185283DNAPopulus tremula x tremuloides 185atgggcagat ctccttgttg tgagaaagaa cacaccaaca aaggagcgtg gactaaggaa 60gaagatgaac gtctcattaa ttatatcaaa tctcatggtg aaggttgttg gaggtctctc 120cctaaggcag ctggtttgct tcgatgtggc aaaagttgta gactgaggtg gataaactac 180ctgaggcctg atctcaagag aggaaacttc agtgacgagg aagatgaact catcatcaac 240ctacacagct tacttggaaa caagtggtct ctcattgctg cta 283186379DNAPopulus tremula x tremuloides 186atggaggttt atccataccc atctcgtttt tccatgtctt cattgtgttc ttttgggaat 60tttgttgata aggttaaaga agtttgtaac ttcgttgttt cagctattat tggcaacata 120ttctctgcga tcttcacctt tttctttgca ttagtgggca ctttgttagg agccatgact 180ggggcattga taggccaaga aactgaaagt gggtttgttc ggggggctgc agttggagcc 240atatcaggag ctgttttctc aattgaagta ttcgagtcat ctcttgttct ttggcaatca 300gatgaatctg ggataggctg tgtcctttac ttgattgatg ttatcgcaag ccttcttagt 360ggacgacttg ttcgtgagc 379187266DNAPopulus tremula x tremuloides 187atgagaatat ctagagcaag atggttcacc ttcttgagaa gagtattcca ctaccagaat 60ggctcaaggt ctaatcttgg gtctaatcct ttcaactcca gcacttggat gatgctggag 120tttgtagctt tggtcattca aataagtatt accatgttca ccctggctat ttccaaggca 180gagaagccag tttggccagt gagaatatgg attattggct ataatattgg ttgtgtcctt 240agtctgctgc tgctctatgg gcgata 266188255DNAPopulus tremula x tremuloides 188atgggcgagg aagcagaccc aacaatgaag aagaagaaga aaaagggacg tccttctctt 60ctagagcttc aaaaacgctc catcaaacaa caacagcttc agcaaacaac ccctatttct 120cttaaaaacc ctaatcctct caactccaat tctgtcctcc ctaaccgtcg atccgctcgc 180cggagctcta attcctatgc accggagtgg attgacggag acgacgaaga agatgacgac 240gaagacgatg agcgt 255189273DNAPopulus tremula x tremuloides 189atgaagtcac cggcaaccgg tgccggagga accgcggcaa cctccacagc taccaacggt 60gaaggggcag agaagaagag cataaaccct gagttatggc aagcatgtgc aggaccgcta 120gtgaacttgc cggcggccgg aactcatgtt gtctacttcc ctcaagggca tagtgaacag 180gtagcagcat ctttgaagaa ggacgtgaat gcgcaaattc caaactaccc aaatcttcca 240tcgaagcttc tatgtctcct tcacaatgtc acc 273190456DNAPopulus tremula x tremuloides 190atggtgggtg gtgagaattt atccagaatg acagtatcta aagcttctgg agaagacaca 60attagcaatg tttcttcttt gggtaaccaa ggcatgccac acactattac agtgccacca 120ccaaagaaga agcgaaatct ccctggaatg ccagatccag atgcagaagt cgttgcgtta 180tcgccaaaga ctttagtagc cacaatcaga ttcgtgtgtg agatctgtaa caagggcttt 240caaagagacc agaacctcca acttcacaga cgaggccata acttaccatg gaagctaaag 300caacgaacca acaaagagcc aaggaagcgt gtctatgttt gtccagagtc atcttgtgta 360catcacaacc cagctagagc tcttggtgat cttactggca ttaaaaagca cttctacaga 420aagcatggtg agaaaaagtg gaagtgtgag agatgt 456191407DNAPopulus tremula x tremuloides 191atggatccca agggctctaa ctcaaaaaac ccacatgagt tacccacttt cttgacccac 60acccaccctt ctcctcctca tcctcctcct cctccacaac cccatcttca acaaccacaa 120caactccata gccaaaacca acaacaaccc aacatgggag acaacaaacc agcagaaatc 180aaagactttc agattgtagt agctgacaaa gaagagcaaa agaaacagtt agcaccaaag 240agaagctcaa acaaagacag acacacaaaa gttgaaggta gaggtagaag gataaggatg 300ccagctcttt gtgcagcgag aatctttcaa ttgacaagag aattgggtca caaatctgat 360ggagagacaa tacagtggct tctacaacaa gctgaaccat ctataat 407192482DNAPopulus tremula x tremuloides 192ttatcgccat gacaaaactg tgagtagtgg cacctcattc caagctaaag ttaagaaccc 60aggctgagaa tcatcaagag aatacaaatc actgtaattg caataccata agagtatctt 120agcctgactc attgcataat ggctaagcga aaccgattca aaacctgaac tttccattaa 180aactccccat tgatctttat cctccatcct ttcccttctc attccaggct cctctggccc 240tacaacacta gaaattctct gacccaataa caacctctca acttgaagcc tctctggtga 300gtctctactc aagttaggct caagaggctc aaaaacagca gtgtagtatc tcaaagcatt 360cttaaaccga gtcaagtacc caacccggtt caaactagct tcatattcac caagagtgac 420aattatcggg ttcaacgatt tagccatctt taaagcagtt tctacagcaa ctggagactc 480at 482193401DNAPopulus tremula x tremuloides 193gtaatcaaat agtagctgtt acttccaagc tgaagcaaca atcaaacttt tgtcctccca 60cccaaaatga agggcaccta cttcctgctt caacatgtat ctatcgcagt actccttaat 120gagtttccgg atcatatcca ccacattagg agtgattgaa catgaagtga agccagccat 180catcatcctt gctctccatt tccctgcaac ttcataccgc tcaatccttt cctctccctc 240acatgcgact atgttcacta tgtcccttgc caggcactgt ttctcaacat tcagtctatc 300ctggctctcc cgtgggagag ttgcatcaag agagtcaaac acagcagagt aatagttgta 360agcttcggta aatctcggga aaaaaggggc agtattagta t 401194373DNAPopulus tremula x tremuloides 194gtcattgttg tgaatcacca gcggcgagct gccacgccga ggtggcaatt agtggccttg 60tatgccaccc aagcatgaga cagccattat tctcctccac cctatacccg tcaccaccgg 120caaagagggc caacaacata ctagcttgtt taaaggcatt cgacccaaga tgaaccgggt 180caaacccagc cgaatcaaac cgagttctcc actgagccaa cgtctcgtgt cgctcaactc 240ggtcagcccc ttcgcatgcc accacattac aaatatgcct ccctaagtac agctcggaca 300tagccaggtc ctgactcggt ggagtcaaac cggacccttc gagagagtca aacaaagtcg 360agtaataatg taa 373195438DNAPopulus tremula x tremuloides 195aagttcagta attccaaagg taatcaagat caactggtcc ggcagagctt gatggatcct 60caaaggacct atgaaccaat tcatggttgg ttaccactga agacatatca gtgctgaggc 120cagtgtcctc tgaaagctcc cgttttgagt ataggttcat gccgggtcca tggttttcaa 180gcaatatcct caacatggag tgttctggca tcataacaga atctgggtat tgaaaacttg 240ctatgtttgg taaaatttga gaggaatata aagagtttgg aggtgagggt tttgaaaaca 300gaactgagga aggggacatg tttgaagaag acaaaggagc tccaagagaa atgttaaagc 360aatcagcaat gatttcatgg ggtttctggt cctccgttgg tttggagaag caggtcacgt 420gagacttatc acctatgt 438196413DNAPopulus tremula x tremuloides 196gcataatcag ctttcacatt ggagagcagc cttgatcttt tggacagtgc atgaaataag 60gttgttgact gtctccacag attctactgt gagcttcgcc gtagggagac tattcaccag 120tatttggaag gccaccgtca aaagggaccc acttaccctc tcttggccac cgacattatt 180agcagtctgg acgccattgg tcgttggggg gccacgtgaa ccaggcccat ccggtacgat 240agcgaaccct gacggaagaa gcgccacgta agccgaatca ccgccgttca tcactacgtg 300catggccgga gtgtcaacgg gagcgtacac tacaagagag cctgctgcat ctatgcatgt 360ctcttgcagt attagcatgc tactctggtt agcattcatg gcgctagcac gta 413197384DNAPopulus tremula x tremuloides 197cctggtggtc atcaagcatc caaaggtctg taaaatctat aaagactatc agctgttaca 60aatttttcca cggcattaca gcaactacat gcttgactgt tcttgaaccc tcgattgtaa 120gaacacaaac atgctgttct atgaacaaca gcaggggcac tggaagtagg agtagctgag 180gtcccaaaca attgatatgt gactacttgt tcttgatgct gttggtttgc ttgctttgaa 240tcattaagat cattgataga agatagttga gattgacatg gaaggccaat agagagctca 300agattgagtt tgtgatttgg atacacttct aattctgctg tcacgccact gctactattg 360gaatcttcag cagagtcact gctg 384198176DNAPopulus tremula x tremuloides 198tcacagatcc cttctacaca gagggcagga tgcatgccta agtagccact tatctatgca 60aggtaggtga aacatgtgat gacaatgagg caagcttctg accgtctctc ccagctgaaa 120gtcctgaagg caaactgaac aagagacttt ctctcctgat tcatctacat tgttat 176199169DNAPopulus tremula x tremuloides 199ctatttttcc agttcttgtt tacagagagg gcagcaggat ataattcgaa gccattgatc 60tacacacttg aggtggaaca tatgggaaca tggcaatttc ctaacttctt ctttgtcttt 120gtacttggct aggcagatac aacattccag atcttcactt gcaatggtt 169200255DNAPopulus tremula x tremuloides 200tcagagctgc aatgctaaat ctggctgccg tggtgaaccg atgggcacac tcgaagtagg 60tgaaccaggt gccaagaatc caacattcag atctggtgga aatggaacag taccttgttg 120ctgatatggt aaaaatccct gccaagatga tttgccacca aaccccacat tgccaaatgt 180acgagagtca gagctggagc ctacggctgg agaaattcca ggctgcaata atccacttga 240agtttctgac agcat 255201322DNAPopulus tremula x tremuloides 201ttatgcattg acattatcag aactactgcc ggcttgatta ggaaggacag agttgccaaa 60atctccatcc aagctcatct gttggacttc ttgaggagac aggatcttga tgcagcggac 120acagttcaca aactcttccc aagggtcatc ccccactagc aggacatcat tctcgtgatc 180agtgtaaaca agtttccagc ctatcctttg ttgattttcc aactgtccct ctataccaaa 240actacgagcc agatcctgct taagctcgtc ataacctgaa taccgtgtta tgtcaattga 300acttcctaca gctccacgtt tg 322202433DNAPopulus tremula x tremuloides 202tcaagctcct gtagatccat aaggcttcct ctcaggcaca ccgtcccatg tttcttcaga 60agtccctctc cctcctcctc caccatatgc tgcggcagtg gctgctccaa catgaaagcc 120accactatag gaatttagca aagcagaact agcagcatcc atgccaagcc caagaaggtc 180aagtgtcgtt ggtttgtttc caaacaatga agatgaaccc atcataacat cactggaggg 240aagcccaagg cctagtccag ctgcgacagt ggtgttgttc tccggcttca catcccattt 300attgcagtta ctgtcttgat tagttgaacg gggcaatcct aaaccacgga gaaatgatgg 360gtttgatgtc gttgcaccca tttgtgctgc tttttgaagc aatgcagtgg cagacatggc 420tggttgcgga gat 433203360DNAPopulus tremula x tremuloides 203ttactgccct gatccttgtg aatcatcttt agaaggattt tgttgctggt gctgctgctg 60gtgcactcta gcatgcccca tctgctgata aatctgattc aaagcttgtg gactcaaaac 120cccaatatgg ccgtcctgtg acaacccaag ttccaatcct ggtaactgct gggtaccccc 180acctaaaatt gaagtaaaac tcataggccc catgttgtta ctcggcaagt caaaccctgg 240aaacccaagt ttttgcaaat aacttgaacc accagcagca gcagcttcag tccctatatt 300tgttgttgat ggaccagtgg aattagatga tgactggaac ccataacctc caactggggg 360

Claims

1. A method of producing a tree having a modulated growth and/or biomass compared to its wild type, said method comprising transforming said tree with a nucleic acid comprising a promoter operably linked to a polynucleotide encoding a functional gene product comprising a polypeptide sequence selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134, and variants thereof comprising a conservative substitution, wherein an increased level of the functional gene product results in a modulated growth and/or biomass in said tree.

2. The method according to claim 1, comprising: (i) providing an expression vector comprising a nucleic acid that encodes a polypeptide selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134 and at least one regulatory element operably linked to the nucleotide sequence; (ii) introducing the expression vector into at least one plant; and (iii) selecting at least one transgenic plant that has a modulated growth and/or biomass compared to its wild type.

3. The method according to claim 1, comprising (i) selecting plant species with high expression of the polypeptide selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134; (ii) crossing a plant species selected in (i) with the same or another plant species selected in (i), (iii) selecting plants with increased expression of the polypeptide selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134 compared to the plant species selected under (i); (iv) backcrossing one or more times the plants obtained in (iii) and selecting plants with increased expression of the polypeptide selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134 compared to any of the plant species used in (i) and/or plants obtained in (iii).

4. The method according to claim 1, wherein the nucleic acid encodes a polypeptide comprising a conservatively substituted variant selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134.

5. The method according to claim 1, wherein the nucleic acid which encodes the polypeptide shown as a sequence selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134 comprises a silent substitution.

6. A tree having a modulated growth and/or biomass compared to its wild type, said tree being transformed with a nucleic acid comprising a promoter operably linked to a polynucleotide encoding a functional gene product comprising a polypeptide sequence selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134, and variants thereof comprising a conservative substitution, wherein said increased level of the functional gene product results in a modulated in growth and/or biomass in said tree

7. The tree according to claim 6, wherein the nucleic acid encodes a conservatively substituted variant of a polypeptide selected from the group consisting of SEQ ID NO: 14-23, 25, 26, and 116-134.

8. The tree according to claim 6, wherein the nucleic acid comprises a silent substitution.

9. A tree cell or plant progeny of the tree according to claim 6, wherein said tree cell or said plant progeny of the tree comprises said functional gene product.

10. Wood produced by the tree according to claim 6.