RECOMBINANT PROTEINS OF PARAPOXVIRUS OVIS AND PHARMACEUTICAL COMPOSITIONS THEREFROM

Abstract

The invention relates to polynucleotides coding for the PPVO viral genome, to fragments of the polynucleotides coding for the PPVO genome and to polynucleotides coding for individual open reading frames (ORFs) of the PPVO viral genome. The invention also relates to recombinant proteins expressed from the above mentioned polynucleotides and to fragments of said recombinant proteins, and to the use of said recombinant proteins or fragments for the preparation of pharmaceutical compositions.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a continuation of U.S. patent application Ser. No. 13/717,640, filed 17 Dec. 2012, now allowed, which is a continuation of U.S. patent application Ser. No. 10/481,112, filed 11 Jun. 2004, now patented as U.S. Pat. No. 8,357,363, which is a U.S. National Phase Application of International Patent Application No. PCT/EP2002/006440, having an international filing date of 12 Jun. 2002, which claims priority to New Zealand Patent Application No. 512341 filed 13 Jun. 2001. The contents of each of these applications are incorporated herein by reference in their entirety.

SUBMISSION OF SEQUENCE LISTING ON ASCII TEXT FILE

[0002] The content of the following submission on ASCII text file is incorporated herein by reference in its entirety: a computer readable form (CRF) of the Sequence Listing (file name: 595282000303SeqList.txt, date recorded: 5 Sep. 2014, size: 234,776 bytes).

FIELD OF THE INVENTION

[0003] The present invention relates to polynucleotides and recombinant proteins of Parapoxvirus ovis (PPVO) and their use, alone or in combination with other substances, for the manufacture of pharmaceutical compositions.

BACKGROUND OF THE INVENTION

[0004] It is known that latent and chronically persistent viral infections can be activated or reactivated by immunosuppression, or conversely that the immune system suppresses acute diseases which may be caused by a latent virus (for example a latent herpes virus infection recurs as a result of immunosuppression in the form of lip vesicles in cases of stress or the administration of cortisone). It is also known that chronically persistent latent viral infections can only be treated with difficulty or not at all using conventional low-molecular-weight antiviral substances.

[0005] It was demonstrated that class I restricted cytotoxic T cells were capable of inhibiting hepatocellular HBV gene expression in HBV-transgenic mice, and that this process was caused by TNF-α and IFN-γ.

[0006] It is also known that in the case of chronically persistent viral infections a superinfection with another virus can produce antiviral effects against the chronically persistent virus. The dependence of this effect on interferons such as IFN-γ, as well as other cytokines and chemokines, such as TNF-α, which are secreted by T cells, natural killer cells and macrophages, has been demonstrated.

[0007] BAYPAMUN®, a pharmaceutical product for inducing "paraspecific immunity", i.e., a pharmaceutical product for inducing the unspecific immune system, is used therapeutically, metaphylactically and prophylactically for the treatment of animals in need. BAYPAMUN® is manufactured from chemically inactivated PPVO strain D1701 (see German Patent DE3504940). The inactivated PPVO induces in animals non-specific protection against infections with the most diverse types of pathogens. It is assumed that this protection is mediated via various mechanisms in the body's own defense system. These mechanisms include the induction of interferons, the activation of natural killer cells, the induction of "colony-stimulating activity" (CSA) and the stimulation of lymphocyte proliferation. Earlier investigations of the mechanism of action demonstrated the stimulation of interleukin-2 and interferon-α.

[0008] The processes for the production of the above-mentioned pharmaceutical compositions are based on the replication of the virus in cultures of suitable host cells.

[0009] One aspect of the invention relates to the use of particle-like structures comprising recombinant proteins of the invention. These particle-like structures can be, e.g., fusion proteins, protein-coated particles or virus-like particles.

[0010] Methods to produce fusion proteins, protein-coated particles or virus-like particles comprising recombinant proteins of the invention are well known to persons skilled in the art: Casal (Biotechnol. Genet. Eng. Rev. (2001) 18:73-87) describes the use of baculovirus expression systems for the generation of virus-like particles. Ellis (Curr. Opin. Biotechnol. (1996) 7(6):646-652) presents methods to produce virus-like particles and the application of suitable adjuvants. Roy (Intervirology (1996) 39(1-2):62-71) presents genetically engineered particulate virus-like structures and their use as vaccine delivery systems. Methods to produce fusion proteins are also well known to the person skilled in the art (Gaudin, et al., Gen. Virol. (1995) 76:1541-1556; Hughson, Curr. Biol. (1995) 5(3):365-374; Uhlen, et al., Curr. Opin. Biotechnol. (1992) 3(4):363-369). Known to the person skilled in the art is also the preparation of protein-coated micro- and nanospheres (Arshady, Biomaterials (1993) 14(1):5-15). Proteins can be attached to biodegradable microspheres (Cleland, Pharm Biotechnol. (1997) 10:1-43) or attached to other polymer microspheres (Hanes, et al., Pharm. Biotechnol. (1995) 6:389-412) such as, e.g., polysaccharides (Janes, et al., Adv. Drug Deliv. Rev. (2001) 47(1):83-97).

[0011] PPVO NZ2 is another Parapoxvirus strain that exhibits immunostimulatory effects when administered in inactivated form to mammals.

[0012] The closest prior art describes the construction of an expression library representing about 95% of the PPVO NZ2 genome using the Vaccina lister virus to create recombinant viruses comprising the complete Vaccina lister genome and various fragments of the PPVO genome (Mercer, et al., Virology, (1997) 229:193-200). For the construction of the library, 16 PPVO DNA fragments with an average size of 11.4 kb were inserted into the Vaccinia lister genome. Each fragment was mapped relative to the PPVO restriction endonuclease maps but was otherwise uncharacterized (FIG. 1). It was found that a major portion of the PPVO genes were expressed in cells infected by the recombinant virus. The authors also showed that the entirety of all PPVO proteins expressed by some of the recombinant viruses of the expression library was able to provide protection against challenge with virulent PPVO. Expression of PPVO genes of the individual recombinant viruses has been demonstrated by immunofluorescence and immune precipitation (Mercer, et al., Virology (1997) 229:193-200).

[0013] To identify components of PPVO responsible for the vaccinating activity of PPVO, the Vaccinia lister/PPVO NZ2 expression library was applied.

[0014] Based on the above background it was desirable to develop PPVO based pharmaceutical compositions with antiviral and anti-tumor efficacy as well as with efficacy in paraimmunization and other desirable therapeutic effects. It was also desirable to obtain a pharmaceutical composition that exerts its full therapeutic effect while showing fewer side effects. It was furthermore desirable to find methods to produce PPVO based pharmaceutical compositions in large quantities and in economically advantageous manners.

[0015] These desirable effects have been achieved by the systematic use of selected recombinant proteins of PPVO alone or in combination with other recombinant proteins from PPVO for the preparation of pharmaceutical compositions for the treatment of objects in need.

SUMMARY OF THE INVENTION

[0016] The invention relates to polynucleotides coding for the PPVO viral genome, to fragments of the polynucleotides coding for the PPVO genome and to polynucleotides coding for individual open reading frames (ORFs) of the PPVO viral genome. The invention also relates to fragments of said polynucleotides of at least 15 or 30 or 100 base pairs in length. The invention also relates to recombinant proteins expressed from the above mentioned polynucleotides and to fragments of said recombinant proteins of at least 5 or 10 or 30 amino acids, and to the use of recombinant proteins or fragments for the preparation of pharmaceutical compositions.

[0017] "Fragments" of a polynucleotide, within the meaning of the invention, shall be understood as polynucleotides that have the same nucleotide sequence as contiguous parts of the full length (the original) polynucleotide.

[0018] "Active fragments", within the meaning of the invention, shall be those fragments of the PPVO genome the expression products of which have demonstrated to be pharmacologically active according to the invention, when inserted into the Vaccina lister genome and expressed in a suitable host.

[0019] Whereas the use of the complete PPVO virus for the manufacture of vaccines against PPVO challenge has been described, the present invention relates to the use of polynucleotides coding for the PPVO viral genome and selected fragments of the PPVO viral genome and of selected PPVO expression products, alone or in combination with others, for the preparation of improved pharmaceutical compositions for the treatment of various diseases.

[0020] The systematic use of selected genomic fragments of PPVO and their recombinant expression products makes it possible to produce pharmaceutical compositions which contain fewer (and may not contain any) inactive components (i.e., polynucleotides and proteins of PPVO) in addition to the active components.

[0021] These pharmaceutical compositions which contain less, or do not contain any additional inactive components are generally preferred by doctors and patients compared to the less well defined biological preparations of inactivated virus material. Furthermore, the possibility of producing the recombinant product in fermentation processes allows an economically advantageous mode of production. It is well known to persons skilled in the art that an economically advantageous mode of production can be achieved, e.g., by using rapidly growing production organisms (host organisms) which might also place low demands on the culture medium employed. Microorganisms which can advantageously be used as hosts for the production of recombinant proteins include, e.g., but are not limited to, Escherichia coli, Bacillus spec., Corynebacterium spec., Streptomyces spec., as well as yeasts, e.g., Saccharomyces cerevisiae, Candida spec., Pichia spec., Hansenula spec., and filamentous fungi, e.g., Aspergillus spec., Penicillium spec. and other suitable microorganisms.

[0022] Recombinant proteins of the invention can also be produced from cell lines expressing the proteins of interest. These cell lines can be recombinant mammalian cell lines, recombinant insect cell lines (e.g., using the baculovirus transfection system) or other suitable expression systems. Transfection can be achieved by various techniques known to the skilled person, one of which is the use or recombinant viruses such as the Vaccinia virus/PPVO recombinants (VVOVs) described in the examples.

DESCRIPTION OF THE INVENTION

[0023] The invention relates to fragments of the PPVO genome of at least 15 or 30 or 100 base pairs in length, and recombinant proteins expressed therefrom and to the use of said fragments and recombinant proteins for the preparation of pharmaceutical compositions. The invention also relates to individual genes (ORFs) of PPVO and their expression products, and their use, alone or in combination with others, for the preparation of pharmaceutical compositions.

[0024] A protein, within the meaning of the invention, is any polypeptide of at least five amino acids. A recombinant protein, within the meaning of the invention, is any protein that is expressed in a cell, to which the coding polynucleotide was introduced using recombinant DNA technology.

[0025] A polynucleotide, within the meaning of the invention, is meant to comprise, polyribonucleotides and/or polydesoxyribonucleotides.

[0026] Pharmaceutical compositions of the invention can be used as immunotherapeutic or immunoprophylactic agents for the treatment of infectious and non-infectious immunodeficiencies. They can also be used for the treatment of tumor diseases, cancer, viral infections and diseases associated therewith, such as, e.g., hepatitis, papillomatosis, herpes virus infections, liver fibrosis, for the prevention or prophylaxis of infectious diseases after stress (e.g., operations), for the prevention and prophylaxis of infectious diseases by administration prior to operations or procedures (e.g., preceding implantations of artificial limbs or dental procedures), for the prophylactic and metaphylactic treatment of non-viral infections, for the healing of wounds, and in particular for accelerating wound-healing processes and for promoting the healing of poorly healing or non-healing wounds (e.g., Ulcus cruris), for diseases such as multiple sclerosis, warts and other skin neoplasms, for allergic diseases, for preventing the onset of systemic allergies and for topical allergies and for improving well-being, e.g., in old age, for autoimmune diseases, chronic inflammatory diseases, such as, e.g., Crohn's disease, COPD and asthma. It is an object of the invention to use of polynucleotides and recombinant proteins of PPVO for the production of pharmaceutical compositions for the treatment of the above mentioned conditions and diseases in humans and animals.

[0027] The viral strains of the invention are PPVO NZ2 and homologues, such as D1701, NZ7, NZ10 and orf-11 strains. It is also possible to use polynucleotides and recombinant proteins of the progeny of these strains obtained by passaging and/or adaptation using specific cells, such as, e.g., WI-38, MRC-5 or Vero cells.

[0028] We have found that the identified recombinant proteins are effective for the treatment of viral diseases, cancer and other diseases or conditions in which a Th1 type immune response is of benefit. The results obtained also imply that PPVO gene products or parts thereof protect hepatitis virus-expressing hepatocytes (e.g., hepatitis B virus, HBV, or hepatitis C virus, HCV) from immune attack through HBV or HCV specific cytotoxic CD8+ T cells circulating in the blood because T cells will not leave the blood stream if their specific antigen is not presented by liver sinus endothelial cells (LSEC, that anatomically separate hepatocytes from T cells passing the liver with the blood). Therefore, we expect to have a recombinant protein that is derived from the ORFs 120-R3 (base pairs 122616-136025 Bp, recombinant virus VVOV82) that is able to down-modulate or prevent side effects such as necroinflammatory liver disease when immunostimulants, e.g., cytokines or any others including the proteins described above administered to, e.g., hepatitis patients.

[0029] Considering the knowledge about the influence of a Th1 type immune induction in conditions and diseases such as latent and or chronic viral infections, proliferative diseases such as cancer and the capability of recombinant proteins that contain gene products of PPVO or parts thereof to induce a Th1 immune response or a local immune response selectively, we claim the use of polynucleotides and recombinant polypeptides of PPVO and recombinant proteins that contain gene products of PPVO or parts thereof for the manufacture of pharmaceutical compositions for use in humans and animals. The recombinant proteins are made from products or parts thereof of the following open reading frames (ORFs) of PPVO NZ2: 64r-96r (recombinants VVOV 285 and VVOV 330 as well as VVOV 243 and VVOV 283), 18r-57 (recombinants VVOV 97, VVOV 96 and VVOV 245), 4r-14r (recombinant VVOV 215). The recombinant protein may also be made from gene products or parts thereof of ORFs 120-R3 (recombinant VVOV 82). The proteins may be prepared and used in any combination.

[0030] Recombinant proteins of PPVO within the meaning of the invention shall be understood as proteins that derive from PPVO and are expressed in homologous or heterologous systems other than the systems in which PPVO is naturally produced. Examples for recombinant proteins of PPVO are proteins of PPVO which are expressed using Vaccinia virus vectors and fibroblasts as host cells or baculovirus vectors and insect cells as host cells. Recombinant proteins, within the meaning of the invention, could also be produced in bacterial cells (e.g., Escherichia coli, Bacillus spec., Streptomyces spec.) or in yeast (e.g., Saccharomyces cerevisiae, Candida spec., Pichia pastoris, Hansenula spec.) systems. In these cases, polynucleotides of the PPVO genome would typically be brought into the respective host genome so that PPVO genes are expressed by the host. Recombinant proteins of PPVO could also be expressed by the object in need in the sense of a gene therapy.

[0031] Recombinant proteins, within the meaning of the invention, could also be recombinant virus particles that contain PPVO derived proteins. Recombinant proteins, within the meaning of the invention, could also be in form of viral-like particles that are formed or assembled from PPVO derived proteins. Recombinant proteins, within the meaning of the invention, could also be chimeric proteins that contain PPVO gene products.

[0032] In a preferred embodiment of the invention the recombinant proteins are attached to particle-like structures or be part of particle-like structures.

[0033] In another preferred embodiment of the invention the recombinant proteins are attached to, or part of, fusion proteins.

[0034] In another preferred embodiment of the invention the recombinant proteins are attached to, or part of, protein-coated particles.

[0035] In another preferred embodiment of the invention the recombinant proteins are attached to, or part of, virus-like particles.

[0036] Particle-like structures, such as particle-like fusion proteins, protein-coated particles or virus-like particles can be phagocytosed and processed by monocytes or macrophages. The process of phagocytosis enhances the efficacy of recombinant proteins of the invention in uses within the meaning of the invention.

[0037] A particle-like structure, within the meaning of the invention, is particulate matter in particle-like form of which the average particle size and other characteristics are suitable for medical application. Preferred particle-like structures are, e.g., fusion proteins, protein-coated particles, or virus-like particles.

[0038] Immunomodulating activity is defined as local or systemic suppression and/or stimulation and/or induction of any Th-1 or Th-2 type cytokine response or of any effector function of these cytokines, (e.g., cytolytic or antiviral activity or humoral response) or the modulation of MHC cross-presentation. Immunomodulating activity could also be the induction of apoptosis in antigen presenting cells or recruiting of antigen presenting cells.

[0039] Nucleotides and recombinant proteins of the invention can be administered at the same time or sequentially, administered with other agents and drugs, e.g., with drugs that treat the disease or are supportive, e.g., in the case of cancer therapy with antineoplastic or other anti-cancer agents or/and anti-coagulants or vitamins, pain relief and others.

[0040] The nucleotides and recombinant proteins can be administered systemically (e.g., intravenously, subcutaneously, intramuscularly, intracutaneously, intraperitoneally), locally (e.g., into a tumor) or orally (per os). The recombinant proteins or products thereof should be formulated appropriately, e.g., in a non-pyrogenic solution or suspension for i.v. use or in capsules for implantation or in capsules for per os use. Pharmaceutical compositions of the invention can be administered, e.g., oral, nasal, anal, vaginal etc., as well as parenteral administration. Pharmaceutical compositions of the invention can be in the form of suspensions, solutions, syrups, elixirs or appropriate formulations in polymers as well as liposomes.

[0041] Recombinant proteins of the invention can also be prepared with suitable recombinant cell lines and other cell lines. Alternatively, non-recombinant cell lines, such as WI-38, MRC-5, Vero cells could be infected with recombinant viruses that carry the recombinant genes using viral vectors such as, but not limited to, the Vaccina virus (e.g., Vaccina lister). In addition, other suitable viruses can be used in combination with other suitable cells (e.g., using Vaccinia virus vectors and fibroblasts as host cells or baculovirus vectors and insect cells as host cells). It is advantageous to cultivate the recombinant cell cultures in high-cell-density fermentations to achieve favorable productivity and a good overall process performance.

[0042] The invention relates to purified and isolated polynucleotides with the sequence of SEQ ID NO:1. The invention also relates to purified and isolated polynucleotides of at least 15 or 30 or 100 nucleotides which bind under stringent conditions to the polynucleotide of SEQ ID NO:1 or its complementary sequences.

[0043] Stringent conditions, within the meaning of the invention are 65° C. in a buffer containing 1 mM EDTA, 0.5 M NaHPO₄ (pH 7.2), 7% (w/v) SDS.

[0044] The invention also relates to purified and isolated polynucleotides which comprise the polynucleotide sequence of SEQ ID NO:1 or polynucleotide sequences encoding the same amino acid sequence and fragments of at least 15 or 30 or 100 nucleotides thereof. The invention also relates to recombinant proteins of five and more amino acids encoded by these polynucleotides.

[0045] The invention also relates to purified and isolated polynucleotides which show at least 99%, 95% or 90% or 80% sequence homology to the polynucleotides of the previous paragraph.

[0046] Homology of biological sequences, within the meaning of the invention, shall be understood as the homology between two biological sequences as calculated by the algorithm of Needleman and Wunsch. (J. Mol. Biol. (1970) 48:443-453) using the BLOSUM62 substitution matrix (Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA (1992) 89:10915-10919) for proteins and penalties of +4 and -3 for identical and non-identical bases, respectively, when comparing polynucleotide sequences. For comparison of protein sequences the gap creation penalty and the gap extension penalty are 8 and 2, respectively. For comparison of polynucleotide sequences the gap creation penalty and the gap extension penalty are 20 and 3, respectively.

[0047] The invention also relates to purified and isolated polynucleotides which are active fragments of the PPVO genome, with a sequence selected from a group of sequences consisting of nucleotides 122616-136025 of SEQ ID NO:1 (PPVO insert of VVOV 82), 31003-46845 of SEQ ID NO:1 (PPVO insert of VVOV 96), 24056-33789 of SEQ ID NO:1 (PPVO insert of VVOV 97), 10264-20003 of SEQ ID NO:1 (PPVO insert of VVOV 215), 82324-92502 of SEQ ID NO:1 (PPVO insert of VVOV 243), 47952-66263 of SEQ ID NO:1 (PPVO insert of VVOV 245), 89400-103483 of SEQ ID NO:1 (PPVO insert of VVOV 283), 74804-88576 of SEQ ID NO:1 (PPVO insert of VVOV 285), and 102490-108393 of SEQ ID NO:11 (PPVO insert of VVOV 330).

[0048] The invention also relates to purified and isolated polynucleotide which encode for the same amino acid sequence as the active fragments of the PPVO genome of the previous paragraph and to polynucleotides of at least 15 or 30 or 100 nucleotides binding under stringent conditions to the above mentioned active fragments of the PPVO genome or its complementary sequence.

[0049] The invention also relates to polynucleotides with 99%, 95%, or 90%, or 80% sequence homology to sequences consisting of nucleotides 122616-136025 of SEQ ID NO:1 (PPVO insert of VVOV 82), 31003-46845 of SEQ ID NO:1 (PPVO insert of VVOV 96), 24056-33789 of SEQ ID NO:1 (PPVO insert of VVOV 97), 10264-20003 of SEQ ID NO:1 (PPVO insert of VVOV 215), 82324-92502 of SEQ ID NO:1 (PPVO insert of VVOV 243), 47952-66263 of SEQ ID NO:11 (PPVO insert of VVOV 245), 89400-103483 of SEQ ID NO:1 (PPVO insert of VVOV 283), 74804-88576 of SEQ ID NO:11 (PPVO insert of VVOV 285), and 102490-108393 of SEQ ID NO:11 (PPVO insert of VVOV 330) or the respective complementary sequences.

[0050] The invention also relates to purified and isolated polynucleotide, with a sequence of nucleotides 3 to 539 (ORF L1), 781 to 449 (ORF L2r), 1933 to 1664 (ORF L3r), 3269 to 2790 (ORF L4r), 2799 to 3851 (ORF L5), 2962 to 3753 (ORF L6), 3784 to 3122 (ORF L7r), 4341 to 4129 (ORF L8r), 4904 to 4428 (ORF 1ar), 6517 to 4970 (ORF 1r), 8042 to 6684 (ORF 2r), 9989 to 8070 (ORF 3r), 11195 to 10062 ORF 4r), 11493 to 11227 (ORF 5r), 11802 to 12038 (ORF 6), 12358 to 12080 (ORF 7r), 13980 to 12364 (ORF 8r), 14826 to 14053 (ORF 9ar), 15080 to 15394 (ORF 10), 16838 to 15423 (ORF 11r), 19021 to 16847 (ORF 12r), 19704 to 19156 (ORF 13r), 20314 to 19736 (ORF 14r), 20401 to 22101 (ORF 15), 22125 to 22940 (ORF 6), 23003 to 23866 (ORF 17), 26908 to 23873 (ORF 18r), 26926 to 27213 (ORF 19), 27626 to 27216 (ORF 20r), 29754 to 27616 (ORF 21r), 32217 to 29800 (ORF 22r), 33380 to 32418 (ORF 23r), 33602 to 33393 (ORF 24r), 34466 to 33612 (ORF 25r), 34735 to 34502 (ORF 26r), 35905 to 34739 (ORF 27r), 37194 to 35905 (ORF 28r), 37200 to 39248 (ORF 29), 41037 to 39229 (ORF 30r), 41374 to 42066 (ORF 31), 42336 to 41731 (ORF 32r), 42407 to 41997 (ORF 33r), 42410 to 43765 (ORF 34), 43770 to 43958 (ORF 35), 43980 to 44534 (ORF 36), 45727 to 44537 (ORF 37r), 45760 to 46557 (ORF 38), 46567 to 47568 (ORF 39), 47572 to 48303 (ORF 40), 48352 to 48621 (ORF 41), 49887 to 48634 (ORF 42r), 49917 to 50693 (ORF 43), 50719 to 51102 (ORF 44), 51059 to 51511 (ORF 44a), 51584 to 52591 (ORF 45), 52509 to 53066 (ORF 46), 53523 to 53023 (ORF 47r), 53607 to 57473 (ORF 48), 58070 to 57528 (ORF 49r), 57700 to 58662 (ORF 50), 59674 to 58673 (ORF 51r), 62089 to 59678 (ORF 52r), 62198 to 62881 (ORF 53), 62909 to 63862 (ORF 55), 63858 to 64271 (ORF 56), 64309 to 66831 (ORF 57), 67266 to 66799 (ORF 58r), 67803 to 67273 (ORF 58ar), 67915 to 68607 (ORF 59), 68624 to 70984 (ORF 60), 70994 to 72898 (ORF 61), 72938 to 73507 (ORF 62), 73540 to 74211 (ORF 63), 76120 to 74207 (ORF 64r), 76749 to 76186 (ORF 65r), 77698 to 76799 (ORF 66r), 79343 to 77709 (ORF 67r), 79816 to 79367 (ORF 68r), 80529 to 79858 (ORF 69r), 80774 to 80529 (ORF 70r), 82815 to 80788 (ORF 71r), 83835 to 82834 (ORF 72r), 83874 to 85583 (ORF 73), 85535 to 84402 (ORF 74r), 88096 to 85574 (ORF 75r), 87759 to 88667 (ORF 76), 88920 to 88642 (ORF 77r), 91652 to 88938 (ORF 78r), 91667 to 92674 (ORF 79), 93466 to 92681 (ORF 80r), 93761 to 93486 (ORF 81r), 94060 to 93788 (ORF 82r), 94238 to 94080 (ORF 83r), 94508 to 94242 (ORF 84r), 95571 to 94498 (ORF 85r), 96187 to 95600 (ORF 86r), 96202 to 97665 (ORF 87), 97915 to 97643 (ORF 88r), 98251 to 99537 (ORF 89), 99537 to 99974 (ORF 90), 100001 to 101140 (ORF 91), 101168 to 104650 (ORF 92), 106354 to 104795 (ORF 93r), 107947 to 106400 (ORF 94r), 108256 to 107990 ORF 95r), 108719 to 108300 (ORF 96r), 109679 to 108738 (ORF 97r), 109861 to 109682 (ORF 98r), 110830 to 10033 (ORF 99r), 110208 to 110417 (ORF 100), 110469 to 110651 (ORF 100a), 110915 to 111397 (ORF 101), 111419 to 111913 (ORF 102), 111949 to 112485 (ORF 103), 112593 to 113450 (ORF 104), 113323 to 112967 ORF 105r), 113526 to 114152 (ORF 106), 114199 to 115236 (ORF 107), 115353 to 115787 (ORF 108), 115859 o 116551 (ORF 109), 116729 to 117523 (ORF 110), 117572 to 117114 (ORF 111r), 117423 to 118085 (ORF 12), 118968 to 118375 (ORF 114r), 118508 to 119119 (ORF 115), 119588 to 120202 (ORF 116), 120314 to 21231 (ORF 117), 121380 to 123920 (ORF 118), 121288 to 122256 (ORF 119), 122350 to 123924 (ORF 120), 123962 to 125566 (ORF 121), 125193 to 124591 (ORF 122r), 125689 to 123935 (ORF 123r), 123839 to 123297 ORF 123ar), 125652 to 126170 (ORF 124), 126121 to 125699 (ORF 125r), 126279 to 127769 (ORF 126), 127851 to 128408 (ORF 127), 128520 to 130076 (ORF 128), 130105 to 131700 (ORF 129), 131790 to 133283 (ORF 130), 133246 to 133920 (ORF 131), 133972 to 134370 (ORF 132), 134418 to 134693 (ORF 133a), 134402 to 134992 (ORF R1), 134853 to 134419 (ORF R2r), 135628 to 135897 (ORF R3), 136780 to 137112 ORF R4), and 137558 to 137022 (ORF R5r) of SEQ ID NO:1, which encode for the identified open reading frames (ORFs) listed in Table 7. ORFs of this paragraph of which the start position is a larger number than the stop position are coded by the complementary sequence of SEQ ID NO:1. The names of these ORFs end with the letter "r". The invention also relates to the complementary sequences of the sequences of this paragraph.

[0051] The invention also relates to polynucleotides which encode for the same amino acid sequence as encoded by the identified ORFs of the previous paragraph. The invention also relates to polynucleotides of at least 15, 30 or 100 nucleotides binding under stringent conditions to the identified ORFs. The invention also relates to polynucleotides which show at least 99%, 95% or 90% or 80% sequence homology to the sequences of the previous paragraph or which are functional variants a sequence of the previous paragraph.

[0052] A functional variant of a gene, within the meaning of the invention, shall be defined as a gene which is at least 99%, or 95%, or 90%, or 80% homologous to the first gene and which has a similar biological function as the first gene. A functional variant of a gene can also be a second gene encoding the same amino acid sequence as does the first gene (or as does a functional variant thereof), employing the degeneration of the genetic code. A functional variant of a gene can also be a polynucleotide comprising the same sequence as has said gene, however said polynucleotide being shorter (i.e., by means of deletions of one or several nucleotides at one or both ends of the polynucleotide) or said polynucleotide having additional nucleotides at one or both ends of the identical part of the polynucleotide.

[0053] A functional variant of a protein, within the meaning of the invention, shall be defined as another protein which is at least 99%, or 95%, or 90%, or 80% homologous to the first protein and which has a similar biological function as has the original protein.

[0054] The invention also relates to recombinant proteins encoded by nucleotides of the invention and parts and fragments of said proteins which are at least 5 or 7 or 10 or 30 amino acids long.

[0055] The invention also relates to recombinant proteins encoded by nucleotides of the invention and parts and fragments of said proteins which are at least 5 or 7 or 10 or 30 amino acids long, said recombinant proteins being attached to a carrier protein or to another carrier. Attaching a protein to a carrier protein can improve or strengthen the immune response to said protein, thereby enhancing the therapeutic or prophylactic effect of administering said protein to a subject.

[0056] The invention also relates to vectors containing polynucleotides of the invention and cells containing these vectors or polynucleotides of the invention.

[0057] The invention also relates to the use of recombinant proteins and polynucleotides of the invention, alone or in combination with at least one other recombinant protein or polynucleotide of the invention for the manufacture of pharmaceutical compositions.

[0058] Combinations of recombinant proteins (or polynucleotides) according to the invention, comprise

[0059] combinations of at least two recombinant proteins encoded by SEQ ID NO:1 (or combinations of at least two fragments of a polynucleotide of SEQ ID NO:1),

[0060] combinations of at least two recombinant proteins encoded by the same active fragment of the PPVO genome, i.e., two or more recombinant proteins encoded by the same VVOV of

[0061] Table 3, Table 4, Table 5, and Table 6 (or combinations of at least two fragments of the same active fragment (VVOV) of the PPVO genome),

[0062] combinations of at least two recombinant proteins, encoded by at least two distinct active fragments of the PPVO genome, i.e., from distinct VVOVs of

[0063] Table 3, Table 4, Table 5, and Table 6 (or combinations of at least two fragments of at least two distinct active fragments (VVOVs) of the PPVO genome), or

[0064] combinations of at least two distinct recombinant proteins encoded by ORFs of Table 7 (or combinations of at least two polynucleotides with the sequence of any of the ORFs listed in Table 7).

[0065] The invention also relates to the use of recombinant viruses comprising the Vaccina lister genome and selected fragments of the PPVO genome for the manufacture of pharmaceutical compositions.

[0066] The invention also relates to the use of recombinant proteins and polynucleotides of the invention for the manufacture of pharmaceutical compositions for the treatment of virus related diseases, viral infections, non-viral infections, proliferative diseases, inflammatory diseases, allergic diseases, and autoimmune diseases.

[0067] Viral infections, within the meaning of the invention, shell be understood as diseases associated with viral infections of the human or animal body, such as hepatitis, papillomatosis, herpes virus infections, liver fibrosis, HIV infections, AIDS, and influenza.

[0068] Non-viral infections, within the meaning of the invention, shell be understood as diseases associated with non-viral infections of the human or animal body, such as infections with mycobacteria, mycoplasma, amoeba, and plasmodia.

[0069] Proliferative diseases, within the meaning of the invention, shell be understood as diseases associated with proliferative disorders, such as cancer, leukemia, warts, tumor diseases, and other skin neoplasms.

[0070] Inflammatory diseases, within the meaning of the invention, shell be understood as diseases associated with acute or chronic inflammatory conditions, such as inflammation of the skin or organs, Crohn's disease, COPD, asthma, but also conditions related to the healing of wounds, e.g., Ulcus cruris, and others.

[0071] Allergic diseases, within the meaning of the invention, shell be understood as comprising both systemic and topical allergies.

[0072] Autoimmune diseases within the meaning of the invention, shell be understood as comprising systemic lupus erythematosus, Sjogren's syndrome, Hashimoto's thyroiditis, rheumatoid arthritis, and juvenile diabetes mellitus, and other autoimmune diseases.

[0073] The invention also relates to the use of recombinant viruses comprising a Vaccinia lister genome and fragments of a PPVO genome for the manufacture of pharmaceutical compositions.

[0074] The invention also relates to the use of recombinant viruses comprising a Vaccinia lister genome and at least one heterologous gene to express at least one heterologous gene in a subject, e.g., for prophylactic and/or therapeutic purposes.

[0075] The invention also relates to the use of a recombinant viruses comprising a Vaccinia lister genome and at least one heterologous gene for gene therapy.

[0076] "Gene therapy", within the meaning of the invention, shall be understood as the act of administering to a subject polynucleotides (and, if necessary, suitable adjuvants or suitable carriers) for the purpose of obtaining a prophylactic or therapeutic effect in said subject. Typically, the polynucleotides administered are expressed in the subject and the expressed gene products exert a prophylactic or therapeutic effect.

[0077] The invention also relates to

[0078] (a) a particle-like structure comprising a recombinant polypeptide encoded by an open reading frame (ORF) of the polynucleotide of SEQ ID NO:1 or functional variants of said polypeptides,

[0079] (b) the use of a particle-like structure of (a) for the preparation of a medicament,

[0080] (c) the use of a particle-like structure of (a) for the preparation of a medicament for the treatment of virus related diseases, viral infections, non-viral infections, proliferative diseases, inflammatory diseases, allergic diseases, and/or autoimmune diseases,

[0081] (d) pharmaceutical compositions comprising a particle-like structure of (a), and to

[0082] (e) pharmaceutical compositions comprising a particle-like structure of (a) for the treatment of virus related diseases, viral infections, non-viral infections, proliferative diseases, inflammatory diseases, allergic diseases, and/or autoimmune diseases.

BRIEF DESCRIPTION OF THE FIGURE

[0083] FIG. 1 shows the genomic locations of the DNA fragments constituting the insertion library. The position of each DNA fragment is shown against the KpnI map of PPVO NZ2 (Mercer, et al., Virology (1997) 229:193-200).

EXAMPLES

Example 1

Determination of the Integrated PPVO Fragments in the Active VVOVs

[0084] DNA preparation from Vaccinia lister/PPVO recombinants was performed as follows:

[0085] BK-KL 3A cells were grown to confluency in 175 cm² flasks (Becton Dickson Labware, Heidelberg, Germany). Cells were infected with a recombinant Vaccina lister/PPVO virus (VVOV) of Mercer, et al. (Virology (1997) 229:193-200) at a MOI (multiplicity of infection) of 0.01-0.32 and incubated at 37° C. until 100% CPE (cytopathic effect) had been reached. The infected cells were frozen at -80° C., thawed and processed as follows, with modification to the RNA extraction method of Vilcek, et al. (J. Clin. Microbiol. (1994) 32:2225-2231). Using 2 ml PLG Heavy Eppendorf tubes (Eppendorf, Hamburg, Germany) 0.5 ml aliquots of cellular suspension were incubated with 100 μg Proteinase K (Roche Molecular Biochemicals, Mannheim, Germany) and 50 μl SDS (Sigma-Aldrich, Chemie GmbH, Taufkirchen, Germany) at 56° C. for 25 min 0.5 ml Roti®-Phenol/Chloroform (Carl Roth GmbH, Karlsruhe, Germany) was added and the tubes were inverted for several times. After centrifugation at 12000×g for 10 min, the upper phase was transferred into a fresh tube and two volumes of ethanol (Merck Eurolab GmbH, Darmstadt, Germany) and 1/10 volume of sodium acetate (Sigma-Aldrich, Chemie GmbH, Taufkirchen, Germany) was added. The reagents were mixed several times and stored at -80° C. for 3 h. The tubes were centrifuged at 14000×g for 30 min, the supernatant was decanted and the pellet was air-dried for 5-10 min Finally the DNA pellet was resuspended in 30 μl nuclease free water and stored at -20° C. until used.

[0086] DNA concentration was measured spectrophotometrically on a BioPhotometer 6131 (Eppendorf, Hamburg, Germany) at 260/280 nm. The DNA yield of different sample preparations spanned from 100 ng/ml up to 1 μg/ml.

[0087] Polymerase Chain Reaction (PCR) of Terminal Flanking Regions of the Integrated Fragments in the Vaccinia Lister/PPVO Recombinants was Performed as Follows:

[0088] Three different PCR amplification systems were used for amplifying the terminal flanking regions. Each reaction mixture of 50 μl contained 100 ng-1 μg resuspended DNA and primers (Table 1)) were added in a final concentration of 300 nM. Amplifications were carried out on a Mastercycler® gradient (Eppendorf, Hamburg, Germany).

[0089] The 3-prime flanking region of recombinant VVOV 285 had been analyzed using 2× Ready-Mix® PCR Master Mix (1.5 mM MgCl₂) (AB Gene, Hamburg, Germany). 1 μl BSA (MBI Fermentas GmbH, St. Leon-Rot, Germany) was added to each reaction. Denaturation was performed at 94° C. for 3 min, followed by 30 cycles (94° C. for 30 s, 58.7° C.-65.3° C. for 30 s, 72° C. for 1 mM) and 72° C. for 5 min.

[0090] The 5-prime flanking region of the PPVO insert of recombinant VVOV 285, the 3-prime flanking region of VVOV 97, and both terminal flanking regions of VVOV 215, VVOV 243, VVOV 245 were amplified using PfuTurbo® DNA Polymerase (Stratagene, Amsterdam, Netherlands). The reactions were setup with 2.5 U of enzyme, 1.5 mM MgCl₂ and 200 μM of each dNTP. Denaturation was performed at 94° C. for 3 mM, followed by 30 cycles (94° C. for 30 s, 58.7° C.-65.3° C. for 30 s, 72° C. for 1 mM) and 72° C. for 5 min.

[0091] The amplification of the 5-prime flanking region of VVOV 97 and VVOV 82, the 3-prime flanking region of VVOV 96 and VVOV 283 were performed with Platinium® Pfx DNA Polymerase (Life Technologies GmbH, Karlsruhe, Germany). A reaction of 50 μl contained 1.25 U polymerase, 1-1.5 mM MgCl₂ and 300 μM of each dNTP. Additional use of PCRx Enhancer Solution was necessary for amplification of the 5-prime flanking regions of VVOV 96 (lx concentrated) and the 3-prime flanking regions of VVOV 82 (2× concentrated). Denaturation was performed at 94° C. for 2 mM, followed by 30 cycles (94° C. for 15 s, 54.6° C.-60.7° C. for 30 s, 68° C. for 1-2 mM) and 68° C. for 5-7 min.

[0092] 18 μl of each amplification product was analyzed by agarose gel electrophoresis on 1.5-2% SeaKem LE agarose (Biozym, Hessisch Oldendorf, Germany). After staining in a ethidium bromide solution for 20 mM the DNA fragments were visualized on an UV transilluminator UVT-20 M/W (Herolab, Wiesloch, Germany).

[0093] The sequence of the amplified DNA-fragments were determined by standard sequencing procedures and compared to the published Vaccinia lister thymidine kinase-sequence and the genome sequence of PPVO NZ2 to determine exactly the integrated PPVO NZ2 sequences.

TABLE-US-00001 TABLE 1 PCR-primers, amplification and sequencing of the terminal flanking regions of the integrated fragments in the Vaccinia lister/PPVO NZ2 recombinants Ampli- Length fied of terminal Primers used amplifi- region for amplification SEQ cation VVO of NZ2 Primer Sequence ID product V insert name 5' → 3' NO: [bp] VVO 5' VAC- ATTACAGTGATG 2 264 V 215 P11-1 CCTACATGCCG PPVO GCTGTAGTCGT 3 14r-1 GGTCCGGC 3' PPVO CTTCCTAGGCT 4 402 4r-2 TCTACCGCACG VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT VVO 5' VAC- ATTACAGTGATG 2 553 V 245 P11-1 CCTACATGCCG PPVO CTGGCCAACG 6 57-1 ACGCCTTC 3' PPVO TCTGGTACCCC 7 321 40-1 TTGCCGG VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT VVO 5' VAC- ATTACAGTGAT 2 241 V 285 P11-1 GCCTACATGCCG PPVO GAACCCGCTCT 8 78r-5 CGCTCGA 3' PPVO GCCGGGCAAGT 9 320 64r-1 GTCTGGTC VAC- CGGTTTACGTTG 5 TK-1 AAATGTCCCAT VVO 5' VAC- ATTACAGTGAT 2 392 V 330 P11-1 GCCTACATGCCG PPVO CTCGAAGTAGC 10 92-1 TGATGTCGCG 3' PPVO AGAGCTTTAC 11 462 96r-1 GTAGACTCT CCAAGTGTC VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT VVO 5' VAC- ATACGGAACGGG 12 239 V 96 TK-fwd ACTATGGACG PPVO GCGGTGGCCATG 13 22r-3 TACGTG 3' PPVO GGTTGTGGCGA 14 1055 22r-4 TGGTCGG VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT VVO 5' VAC- ATACGGAACGGG 12 309 V 97 TK-fwd ACTATGGACG PPVO CTTGATGAGCCG 15 18r-1 GACGCA 3' PPVO CCGAGTTGGAG 16 318 25r-1 AGGAAGGAGC VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT VVO 5' VAC- ATTACAGTGAT 2 478 V 243 P11-1 GCCTACATGCCG PPVO CTGTTGGAGGAT 17 79-1 GAGGTCAAGGA 3' PPVO CGTGCTCATGC 18 269 71r-1 CTGTGGAC VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT VVO 5' V 283 3' PPVO CGACATCCTCA 19 234 92-4 CCTGCAAGAAG VAC- CGGTTTACGTTG 5 TK-1 AAATGTCCCAT VVO 5' VAC- ATACGGAACGG 12 275 V 82 TK-fwd GACTATGGACG PPVO TACAGGCAGCC 20 120-1 CGTGACC 3' PPVO GCCGTGTGTC 21 1960 R3R4-3 ACGTTGATGC VAC- CGGTTTACGTT 5 TK-1 GAAATGTCCCAT

Example 2

Induction of Interferon Gamma and Tumor Necrosis Factor Alpha by PPVO Gene Products

[0094] The 16 recombinants were tested of their ability to induce tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) in whole blood cultures.

[0095] Whole blood cultures containing blood and RPMI medium (Life Technologies GmbH, Karlsruhe, Germany) in the ratio of 1:5 were stimulated with the recombinant viruses. A pure Vaccinia lister and a whole PPVO preparation served as controls. All preparations were used at a final dilution of 1:10. The stimulation for the IFN-7 determination was done together with Concanavalin A (SIGMA, St. Louis, Mo.), because the virus alone does not induce IFN-γ. Then the cells were incubated for 24 h (TNF-α) and or 72 h (IFN-γ). The cytokine concentration was then determined in the cell culture supernatants by TNF-α or IFN-γ specific ELISA. These time points were found to be optimal when the experimental conditions were determined using whole PPVO as a control.

[0096] It was possible to identify 5 active recombinant viruses (VVOV 96, VVOV 97, VVOV 243, VVOV 285, and VVOV 330) that induced both TNF-α and IFN-γ secretion and, thus, could mimic the effect of the whole PPVO. The results are depicted in Table 2.

TABLE-US-00002 TABLE 2 TNF-α was determined after 24 h stimulation of blood cells with the recombinant virus or the controls, respectively. IFN-γ was determined after 72 h stimulation of blood cells with the recombinant virus or the controls. Stimulation was performed in the presence of the mitogen ConA. The relative induction in percent of the Vaccinia virus control is shown. Therefore, values greater than 100% are due to the activity of the PPVO fragments. Active PPVO fragments are in bold. The data represent mean values of three different blood donors. Recombinant Virus Clone or Interferon TNF control Induction (%) Induction (%) Vaccinia virus control 100 100 NZ-2 control 2224 264 VVOV 80 200 66 VVOV 82 173 65 VVOV 85 209 94 VVOV 86 138 73 VVOV 96 1638 1016 VVOV 97 1713 1285 VVOV 212 94 62 VVOV 213 192 38 VVOV 215 97 82 VVOV 216 197 71 VVOV 243 1446 933 VVOV 245 98 45 VVOV 247 85 74 VVOV 283 115 78 VVOV 285 1128 1127 VVOV 330 1762 2135

TABLE-US-00003 TABLE 3 The recombinant Vaccinia lister/PPVO viruses that induce both interferon gamma and TNF-α expression are listed in column 1, the corresponding PPVO sequence in column 2 and all open reading frames (ORFs) that are completely or partially contained in the recombinant are depicted in column 3. PPVO NZ2 Sequence PPVO NZ2 ORFs Active recombinant [Bp] that is contained in that are contained in PPVO Vaccinia virus the recombinant the recombinant VVOV 97 24056-33789 18r-25r VVOV 96 31003-46845 22r-39 VVOV 285 74804-88576 64r-76 VVOV 243 82324-92502 71r-79 VVOV 330 102490-108393 .sup. 92-96r

Example 3

Local Immunomodulation by PPVO Gene Products in Liver Sinus Endothelial Cells (LSEC)

[0097] We have established a new cell-based assay system that allows testing of hepatoprotective properties of recombinant PPVO proteins expressed in different systems (e.g., Vaccinia virus). This assay system uses primary murine liver cells, which play the central role in deciding whether immunity or tolerance is induced in the liver, the LSEC. The unique ability of LSEC to present exogenous antigens to CD8+ T cells on MHC class I molecules allows immune surveillance of hepatocytes as viral antigens released by infected hepatocytes are likely to be taken by LSEC and presented to cells of the immune system. The new assay allows to measure the ability of LSEC to interact antigen-specifically with CD8+ T cells, that are responsible for tissue destruction in necroinflammatory hepatitis.

[0098] Pure populations of LSEC are isolated from murine liver by a stepwise procedure of portal-vein perfusion with collagenase A (0.05%), mechanical dispersion and further enzymatic digestion in a rotatory waterbath for 40 min at 37° C. (245 rpm), gradient centrifugation (metrizamide 1.089 g/cm³) and centrifugal elutriation using a Beckman Avanti J25I centrifuge equipped with a JE-6B rotor and a standard elutriation chamber. LSEC cell populations isolated by this method are typically around 95-99% pure as measured by uptake of endothelial cell specific substrate (acetylated low density lipoprotein). LSEC were seeded onto collagen type I coated 24 well tissue culture plates at a density of 100.000 cells per well and were further cultured in Dulbecco's modified Eagle Medium supplemented with 5% fetal calf serum (specially tested not to interfere with the assay system) and 2% glutamine. Three days after isolation, when LSEC gained a post mitotic and quiescent state, we tested for the ability of LSEC to present soluble ovalbumin to (ovalbumin-specific) CD8+ T cells. LSEC were incubated with 1 μM of ovalbumin for three hours (antigen dose and time were previously shown to be optimal for testing of substances suspected to influence antigen-presentation), washed and incubated with a CD8+ T cell hybridoma (200.000 cells/well) that recognizes the peptide SIINFEKL (SEQ ID NO:320). SIINFEKL (SEQ ID NO:320) is recognized in a H2b context and directly binds on the MHC-I molecules. Therefore, it has not to be processed by the cell. This allows to differentiate between accessory functions of LSEC (such as MHC-I expression) and antigen-processing function.

[0099] The extent of CD8+ T cell activation was measured by determining the extent of IL-2 release from T cells by specific sandwich ELISA.

[0100] Using Vaccinia virus expressed recombinant proteins derived from PPVO we have been able to attribute hepatoprotective activity to individual clones. To be able to compare different clones directly with respect to their ability to influence cross-presentation by LSEC, we used equal amounts of "infectious units".

[0101] We found that LSEC cross-present exogenous ovalbumin very efficiently on MHC class I molecules (k^b) to CD8+ T cells. To our surprise we found if LSEC were incubated with several recombinant PPVO proteins we observed subsequently a potent downregulation of cross-presentation by more than 60% compared to the mock-treated control that includes all but the active ingredient. Several regions within the genome of PPVO have immunoregulatory properties. Especially the region termed 82 (43% reduction) which is located at the 3' end of the genome appears to be responsible for the overall effect of PPVO on cross-presentation by LSEC. Further regions (VVOV 215, VVOV 212, VVOV 247 and VVOV 86) bear further immunoregulatory potential, although to a lesser degree (around 30% reduction in cross-presentation). It further appears that genes coding for proteins that downregulate cross-presentation are arranged in clusters. It is of interest to note that we identified two gene clusters coding for proteins that improved cross-presentation (VVOV 330, VVOV 283, VVOV 285, VVOV 97, and VVOV 96). However, for unknown reasons the downregulatory effect of the proteins mentioned above is dominant in the activity of PPVO on cross-presentation.

[0102] Our results strongly suggest that PPVO contains a mixture of different proteins that in a complementary way work to eliminate hepatocytes from hepatitis B virus while conserving hepatic integrity and avoiding long lasting damage secondary to hepatic fibrosis. As PPVO contains a gene with high homology to the anti-inflammatory agent IL-10 (located in the 5-prime region of the genome) we wondered whether the potent downregulatory effect of the clone 82 was due to expression of ovine IL-10. This assumes that there is cross-reactivity between murine and ovine IL-10 at the level of receptor recognition. We have been unable to demonstrate involvement of ovine IL-10 on the immunoregulatory potential of PPVO. Recombinant murine IL-10 did not show any influence on cross-presentation through LSEC and several monoclonal antibodies to murine and human IL-10 did not influence PPVO mediated downregulation of cross-presentation. We conclude that the immunoregulatory component of PPVO is probably not IL-10 but a new, so far not identified mediator. The data for the MHC-I cross-presentation--down-modulating recombinant virus are depicted in Table 4, those for the MHC-I cross-presentation--stimulating recombinant viruses in Table 5.

TABLE-US-00004 TABLE 4 The recombinant Vaccinia lister/PPVO virus that down-modulates the MHC-I cross presentation is designated in column 1, the corresponding PPVO sequence in column 2 and all open reading frames (ORFs) that are completely or partially contained in the recombinant are depicted in column 3. PPVO NZ2 Sequence PPVO NZ2 ORFs Active recombinant [Bp] that is contained in that are contained in PPVO Vaccinia virus the recombinant the recombinant VVOV 82 122616-136025 120-R3

TABLE-US-00005 TABLE 5 The recombinant Vaccinia lister/PPVO viruses that stimulate the MHC-I cross presentation are designated in column 1, the corresponding PPVO sequence in column 2 and all open reading frames (ORFs) that are completely or partially contained in the recombinant are depicted in column 3. PPVO NZ2-Sequence PPVO NZ2-ORFs Active recombinant [Bp] that is contained in that are contained in PPVO Vaccinia virus the recombinant the recombinant VVOV 97 24056-33789 18r-25r VVOV 96 31003-46845 22r-39 VVOV 285 74804-88576 64r-76 VVOV 283 .sup. 89,4-103483 78r-92 VVOV 330 102490-108393 .sup. 92-96r

Example 4

Determination of the Immunostimulatory Activity of the Vaccinia Lister/PPVO Recombinants in the Aujeszky Mouse Model

[0103] We also tested the activity of recombinant Vaccinia lister/PPVO NZ2-viruses in the Aujeszky mouse model, a lethal challenge model of acute Suid Herpesvirus 1 disease for determining the activity of various immunostimulators (e.g., Baypamun®, CpG oligonucleotides).

[0104] a) Conditions Employed for the Mice

[0105] The NMRI mice (outbreed strain HdsWin:NMRI; female; weight: 18-20 g; obtained via Harlan/Winkelmann, Borchen, Germany) were kept in autoclavable polycarbonate crates lined with sawdust in an S2 isolation stall at 20-22° C. (atmospheric humidity: 50-60%) and subjected to an artificial day/night rhythm (illumination from 6:30 h to 18:30 h and darkness from 18:30 h to 6:30 h). They had free access to feed and water.

[0106] b) Challenge Model

[0107] Groups of mice consisting of 10 mice per group were used for the tests. All of the animals in one group were given the same test substance.

[0108] After the mice were supplied they were kept in the animal stall for 2-3 days. Then the Vaccinia lister/PPVO NZ2 recombinants were diluted with PBS (Life Technologies GmbH, Karlsruhe, Germany) to a titer equivalent of approx. 10⁸ TCID₅₀/ml and thermally inactivated (twice for one hour at 58° C.). Of these solutions 0.2 ml was administered per mouse intraperitoneally.

[0109] 24 hours after the treatment the mice were infected with the pseudorabies virus of the Hannover H2 strain by intraperitoneal administration. For this purpose the virus was diluted in PBS to a test titer of approx. 10⁴ TCID₅₀/ml and 0.2 ml of this suspension was administered.

[0110] As a negative control one group of mice was treated with PBS and then infected. The mice in this group died 3-8 days after infection. A large proportion of the mice treated the Vaccinia lister/PPVO NZ2 recombinants VVOV 215, VVOV 245, VVOV 285 or VVOV 330 survived infection with the pseudorabies virus. 10 days after the infection with the virus the test was ended.

[0111] The level of induced immunostimulation was determined by comparing the number of dead mice in the PBS control group with the number of dead mice in the test groups and was quantified by the efficacy index (EI). This index indicates the percentage proportion of mice protected against the lethal effects of the Aujeszky virus infection through immune stimulation by the substance to be tested. It is calculated by means of the following formula:

EI=(b-a)/b×100,

where b is the percentage proportion of the dead mice in the control group and a the percentage proportion of the dead mice in the test group.

[0112] A chi-square test was used for the statistical evaluation. This test reveals the minimum activity indices indicating a significant difference between the mortality rate of those mice treated with the test substance and those treated with PBS. Activity indices of ≧60% are significant where at least 5 of the mice used in tests with n=6 mice per group in the PBS control group and at least 7 of the mice used in tests with n=10 in the PBS control group do not survive the infection with the Aujeszky virus.

[0113] Altogether 3 separate tests were carried out in each case. The testing of Vaccinia lister/PPVO NZ2 recombinants in the Aujeszky mouse model shows the following:

[0114] Surprisingly, after the treatment of the mice with the Vaccinia lister/PPVO NZ2 recombinants VVOV 215, VVOV 245, VVOV 285 or VVOV 330 the average activity indices of higher than 60% demonstrated immunostimulation. By contrast all of the other Vaccinia lister/PPVO NZ2 recombinants were ineffective. The data is summarized in Table 6.

TABLE-US-00006 TABLE 6 The recombinant Vaccinia lister/PPVO viruses that protected mice from herpesvirus induced death are designated in column 1, the corresponding PPVO sequence in column 2 and all open reading frames (ORFs) that are completely or partially contained in the recombinant are depicted in column 3. PPVO NZ2-Sequence PPVO NZ2 ORFs Active recombinant [Bp] that is contained in that are contained in PPVO Vaccinia virus the recombinant the recombinant VVOV 215 10264-20003 .sup. 4r-14r VVOV 245 47952-66263 40r-57 VVOV 285 74804-88576 64r-76 VVOV 330 102490-108393 .sup. 92-96r

TABLE-US-00007 TABLE 7 Sequences of the Parapox ovis open reading frames. ORFs the names of which end with "r" are encoded on the complementary DNA strand. Base pair positions in the "from" and "to" column are relative to SEQ ID NO: 1. SEQ SEQ ID ID ORF from to N-term NO C-term NO Comment L1 3 539 IRGFAG 22 PQKVFRL 23 long termal repeat (LTR)-protein, retroviral pseudoprotease L2r 781 449 MSEGGRL 24 LLGLLFP 25 LTR-protein, retroviral pseudoprotease L3r 1933 1664 MTVHPPK 26 VLPPNSL 27 LTR-protein, retroviral pseudoprotease L4r 3269 2790 MHPSPRR 28 PVSHPFL 29 LTR-protein, retroviral pseudoprotease L5 2799 3851 MGDREGE 30 FEDGVKC 31 LTR-protein, retroviral pseudoprotease L6 2962 3753 MCTVATF 32 GAPRAGW 33 LTR-protein, similar to 134r, retroviral pseudoprotease L7r 3784 3122 MTPTSRE 34 ARTAPPR 35 LTR-protein, retroviral pseudoprotease L8r 4341 4129 MPGEGQY 36 NGGLGKI 37 LTR-protein, retroviral pseudoprotease 1ar 4904 4428 MEFCHTE 38 DTAWYIS 39 dUTPase 1r 6517 4970 MLSRESV 40 RAMLTRP 41 homolog of G1L in NZ2, Ankyrin-repeats 2r 8042 6684 MFFWFWC 42 SGEGVPV 43 3r 9989 8070 MLGFWGK 44 VLPSVSR 45 involved in maturation of EEV (Extracellular Enveloped Virions) 4r 11195 10062 MWPFSSI 46 EFCKPIN 47 Phospholipase D-type enzyme 5r 11493 11227 MLIYGPR 48 RLLKDFP 49 homolog of B3L in NZ2 6 11802 12038 MGVVMCG 50 APAGVTE 51 7r 12358 12080 MPVKVKQ 52 ASREFIV 53 ubiquitination protein with RING- finger-motiv (related to yeast proteins APC11 and HRT1) 8r 13980 12364 MEEELTR 54 SPMVVFN 55 no Vaccinia virus homolog 9ar 14826 14053 MIRIGGG 56 DNMRVDD 57 10 15080 15394 MDGGVHK 58 EQMCRRQ 59 virion core DNA-binding phosphoprotein 11r 16838 15423 MAPPVIE 60 AKNVITH 61 polyA polymerase 12r 19021 16847 MLQLLKR 62 NNRGFRK 63 13r 19704 19156 MACECAS 64 NNCGISF 65 interferon resistance protein, homology to mammalian PACT (protein activator of the interferon-induced protein kinase) also called PRKRA (dsRNA dependent activator of Interferon-induced protein kinase), 13r-protein contains a dsRBD motiv (double- stranded RNA binding domain) and a 'DRADA'- domain that is typical for RNA-editing enzymes) 14r 20314 19736 MDEDRLR 66 KKGKPKS 67 RNA polymerase 15 20401 22101 MDFVRRK 68 VVLQGRA 69 16 22125 22940 MVDSGTH 70 PENVVLL 71 17 23003 23866 MASYISG 72 RTHTVYV 73 18r 26908 23873 MLFEMEL 74 SKPVFTG 75 DNA polymerase 19 26926 27213 MEPRFWG 76 AKVRPLV 77 distant homolog of the ERV1/ALR-protein- family (ERV1: yeast protein, Essential for Respiration and Vegatative growth, ALR: mammalian protein, Aug- menter of Liver Regeneration) 20r 27626 27216 MEAINVF 78 RAYEGML 79 21r 29754 27616 MLLYPKK 80 LLGDGGD 81 related to 12r 22r 32217 29800 MLIRTTD 82 EAQNMQN 83 23r 33380 32418 MEDERLI 84 PSPCGGE 85 24r 33602 33393 MDKLYTG 86 FHYLKLV 87 25r 34466 33612 MKRAVSK 88 LEAPFNI 89 DNA binding phosphoprotein 26r 34735 34502 MESRDLG 90 LNARRQN 91 27r 35905 34739 MNHFFKQ 92 RSLYTVL 93 28r 37194 35905 MDKYTDL 94 PEKPAAP 95 core protein 29 37200 39248 MENHLPD 96 IEAEPPF 97 RNA helicase 30r 41037 39229 MIVLENG 98 RMGARPR 99 Zn-protease, involved in virion morphogenesis 31 41374 42066 MTFRELI 100 DSMASRS 101 late transcription factor 32r 42336 41731 MRGHPAH 102 VAPREEL 103 33r 42407 41997 MASDASP 104 QPSSSRR 105 Glutaredoxin- like enzyme 34 42410 43765 MGIKNLK 106 PRLLKLR 107 35 43770 43958 MVFPIVC 108 LPMLDIS 109 RNA polymerase 36 43980 44534 MREFGLA 110 AEPPWLV 111 37r 45727 44537 MESSKQA 112 TRAPPLF 113 core virion protein precursor 38 45760 46557 MTLRIKL 114 DRSLSCD 115 late transcription factor 39 46567 47568 MGGSVSL 116 YLLIVWL 117 40 47572 48303 MGAAASI 118 TEFPPSV 119 virion protein, related to vaccinia F9L 41 48352 48621 MVRRVLL 120 LCLFSMD 121 42r 49887 48634 MEEKRGR 122 ARAMVCL 123 43 49917 50693 MTNLLSL 124 TGAEAAP 125 core protein, DNA binding domain 44 50719 51102 MAAPTTP 126 VDVLGGR 127 44a 51059 51511 MDHEKYV 128 ATLSPGL 129 45 51584 52591 MEGVEMD 130 RPLRGGK 131 polyA polymerase 46 52509 53066 MNRHNTR 132 SVSVVLD 133 RNA polymerase 47r 53523 53023 MFFRRRA 134 GRRPPRP 135 48 53607 57473 MSVVARV 136 EAAEEEF 137 RNA polymerase chain 1 49r 58070 57528 MGDKSEW 138 FVCDSPS 139 tyrosine phosphatase 50 57700 58662 MAAAPLR 140 ATSGVLT 141 51r 59674 58673 MDPPEIT 142 LLVTAIV 143 immunodominant envelope protein 52r 62089 59678 MDSRESI 144 YMINFNN 145 RNA polymerase- associated trans- cription specificity factor (also called RAP94) 53 62198 62881 MSSWRLK 146 KAAACKK 147 late transcription factor 55 62909 63862 MRALHLS 148 NSEQVNG 149 topoisomerase I 56 63858 64271 MDEALRV 150 FIRAAVA 151 57 64309 66831 MDAPSLD 152 LYVFSKR 153 mRNA capping enzyme 58r 67266 66799 MEPSAMR 154 DVQHVDL 155 virion protein 58ar 67803 67273 MAGFSQS 156 TTCVPPQ 157 59 67915 68607 MATPANA 158 FSFYSEN 159 Uracil DNA glycosylase 60 68624 70984 MAAPICD 160 IEDVENK 161 ATPase, involved in DNA replication 61 70994 72898 MNSDVIK 162 EVSVVNI 163 early transcription factor 62 72938 73507 MSTFRQT 164 ASPAAKN 165 RNA polymerase 63 73540 74211 MRTYTSL 166 WGAAVTR 167 NTP pyrophos- phohydrolase 64r 76120 74207 MTSAHAA 168 VDPASIA 169 virion NTPase 65r 76749 76186 MEGRARF 170 RFCNYCP 171 66r 77698 76799 MKTDCAS 172 KLKLLLQ 173 mRNA capping enzyme 67r 79343 77709 MNNSVVS 174 AEKVTAQ 175 rifampicin resistance, virion membrane 68r 79816 79367 MKRIALS 176 MALKSLI 177 late transactivator protein 69r 80529 79858 MNLRMCG 178 AACSLDL 179 late transactivator protein 70r 80774 80529 MGDNVWF 180 VLGLEQA 181 thioredoxin-like protein 71r 82815 80788 MESPACA 182 NMCDVLC 183 major core protein 72r 83835 82834 MDLRRRF 184 VDNTGTS 185 core protein

73 83874 85583 MEESVAV 186 LLNYGCG 187 RNA-polymerase 74r 85535 84402 MDRLRTC 188 AEAAESA 189 75r 88096 85574 MVSVMRK 190 QEFYPQP 191 early transcription factor 76 87759 88667 MFQPVPD 192 SACRASP 193 77r 88920 88642 MRPCYVT 194 TRGTQTG 195 78r 91652 88938 MTAPNVH 196 AVSFDSE 197 major core protein 79 91667 92674 MTAVPVT 198 VRKLNLI 199 80r 93466 92681 MASEKMA 200 DLDGGMC 201 virion protein 81r 93761 93486 MGLLDAL 202 RFSAASS 203 virion membrane protein 82r 94060 93788 MDIFETL 204 DIELTAR 205 virion membrane protein 83r 94238 94080 MVSDYDP 206 HFVHSVI 207 84r 94508 94242 MFLDSDT 208 DMPFSVV 209 85r 95571 94498 MGDTVSK 210 KTINVSR 211 86r 96187 95600 MESYFSY 212 EDLFFAE 213 virion membrane protein 87 96202 97665 MFGGVQV 214 GRDLAAV 215 RNA helicase 88r 97915 97643 MSAVKAK 216 PLRDLAR 217 Zn-finger protein 89 98251 99537 MTSESDL 218 AIARAQP 219 DNA polymerase processivity factor 90 99537 99974 MIVAAFD 220 NYVLRTN 221 91 100001 101140 MLALFEF 222 LKELLGP 223 intermediate transcription factor 92 101168 104650 MEQALGY 224 SLFSPED 225 RNA polymerase b-chain 93r 106354 104795 MESDNAL 226 GQHAAIW 227 A-type inclusion body/Fusion peptide 94r 107947 106400 MEKLVSD 228 GRSGAIW 229 A-type inclusion body/Fusion peptide 95r 108256 107990 MDENDGE 230 QTGYSRY 231 viral fusion protein 96r 108719 108300 MDAVSAL 232 LFLKSIL 233 97r 109679 108738 MADAPLV 234 RELRANE 235 RNA polymerase subunit 98r 109861 1109682 MEEDLNE 236 MGQASSA 237 99r 110830 110033 MDVVQEV 238 ADSDGGN 239 ATPase 100 110208 110417 MRSWFWQ 240 PLTGMCL 241 100a 110469 110651 MRPKSVG 242 SGHTKPS 243 101 110915 111397 MAHNTFE 244 KYFCVSD 245 enveloped virion glycoprotein 102 111419 111913 MGCCKVP 246 CMKEMHG 247 enveloped virion glycoprotein 103 111949 112485 MSRLQIL 248 RKLDVPI 249 104 112593 113450 MKAVLLL 250 LNLNPGN 251 GM-CSF/IL-2 inhibition factor 105r 113323 112967 MHASLSS 252 DETLTYR 253 106 113526 114152 MEVLVII 254 GEFFYDE 255 107 114199 115236 MPLFRKL 256 RDALDGL 257 108 115353 115787 MACFIEL 258 TTFSSSE 259 109 115859 116551 MSSSSSE 260 TTGTSTS 261 TT 110 116729 117523 MACLRVF 262 CSMQTAR 263 GM-CSF/IL-2 inhibition factor 111r 117572 117114 MAIAHTT 264 FRFRTPG 265 112 117423 118085 MAATIQI 266 KRDGYSR 267 114r 118968 118375 MEGLMPK 268 RPISVQK 269 115 118508 119119 MDSRRLA 270 LGDSDSD 271 116 119588 120202 MRLILAL 272 PQMMRIG 273 117 120314 121231 MAGFLGA 274 CKVEEVL 275 118 121380 123920 MHLHKDP 276 LAFPSLA 277 119 121288 122256 MANRLVF 278 RPMEIDG 279 120 122350 123924 MENNDGN 280 RFLPSHK 281 related to 1r/G1L with Ankyrin- repeats 121 123962 125566 MDPAGQR 282 CSETDRW 283 122r 125193 124591 MSSSAAA 284 IAPDSRM 285 123r 125689 123935 MTAEASI 286 DPVYHKK 287 123ar 123839 123297 MPRTTSG 288 REQTEGL 289 124 125652 126170 MANREEI 290 VRVLRRT 291 125r 126121 125699 MTAPTPR 292 AAYSLAR 293 126 126279 127769 MADEREA 294 LACAMRK 295 related to 1r/G1L with Ankyrin-repeats 127 127851 128408 MSKNKIL 296 SYMTTKM 297 sheep-like Interleukin 10 128 128520 130076 MLTRCYI 298 RASGLAE 299 related to 1r/G1L wih Ankyrin-repeats 129 130105 131700 MVGFDRR 300 CGRRAPE 301 related to 1r/G1L, with Ankyrin-repeats (NT slightly changed) 130 131790 133283 MILARAG 302 PDAAALS 303 Kinase 131 133246 133920 MPPRTPP 304 RPAALRA 305 132 133972 134370 MKLLVGI 306 RPPRRRR 307 homolog to the sheep VEGF (Vascular Endothelial Growth Factor) 133a 134418 134693 MRKKAPR 308 ARTAPPR 309 corresponds to L7r R1 134402 134992 MMRSGHA 310 RMHRSEL 311 LTR-protein (corresponds to L4r), retroviral pseudoprotease R2r 134853 134419 MCTVATF 312 SVAPSSA 313 LTR-protein (corresponds to L6, 134r), retroviral pseudoprotease R3 135628 135897 MTVHPPK 314 VLPPNSL 315 LTR-protein (corresponds to L3r), retroviral pseudoprotease R4 136780 137112 MSEGGRL 316 LLGLLFP 317 LTR-protein (corresponds to L2r), retroviral pseudoprotease R5r 137558 137022 IRGFAGG 318 PQKVFRL 319 LTR-protein (corresponds to L1r), retroviral pseudoprotease

Sequence CWU 1

1

3201137560DNAParapoxvirus ovis NZ2misc_feature(3)...(539)ORF L1 1ggatccgcgg cttcgcgggc ggcggccggc tcccgcggcg gctgagccgc ggtgccgcga 60cgaacgcgga ccaggagttc ctgcgggagg agctacggca gaggctggaa ctgctgaatg 120ctttcgagga cgggcgtccg cgggaacgcg actccgcgga ggcggcaccc cgcagccgcg 180agacctcgct ctggagtcag ggagtcaggg agtcgggagt cagggagtcg ggagtcaggg 240agtcgggagt cagggagtcg ggagtcaggg agtcgggagt cagggagtcg ggagtcaggg 300agtcgggagt cagggagtcg ggagtcaggg agtcgggagt cagggagtcg ggagtcaggg 360agtcgggagt cagggagtcg ggagtcaggg agtcgggagt cagggagtcg ggagtcaggg 420agtcgggagg tcagggagtc gggagtcagg gaaacagaag tccaagtagt acgggtgaga 480caggagtcag gtggtcgggt gaccgcgccg tccgagtccc gcaaaaagtt tttagactct 540gagagaggcc gacctgcctc caacggttcc gcggaaaagt ttttataaaa agttttcggg 600agaggccgac tgccttccaa cggttcctcc tctcgcgtgc cgcgggcggc cgctctcccg 660cgacggtccc gccaacgcgt ttacaaagtt ttaaaagttt tcgagagagg ccgacctgtc 720ttccaacggt tgcgcgaaaa ggttctgcgg aggtttggaa gagccgcccg ccctccgaca 780tcctccgaaa agttttcgca aaaagttttt aaaggtttcg cgaggagttt tcgagggagg 840cgacctgcct ccgaggttcc gcgtaaacgt ttttacaaag cgtcggaggt gggggcgacc 900tgcccttcct ctcctccgaa aagctcttga gagtgtggga gaggcggccg gccttcgcgg 960tcgcgcgaaa aggttctgcg ggagttcgcg ggagctgacc cgccctccgc gcccctccga 1020aaagtttttg cacgaagtct tttggcgttc tcgagaggac gcctaccccg acggtaacgc 1080ggaacgtctc gggaggtcgg cctctccgct cccgcggtcg cggcggcggc ccgtctccgg 1140aggttccgcg aaaagtttta taaaaagttt tgagggaggt accgacctcc taaagtttta 1200cagagagttc tcgcaaaagt tttgggaggt cgacctgacc tcctaaagtt atcgaagagt 1260tctcgcgaag agttctcgca aagagtttga gaggccgacc tgcctccaac ggttccgcgg 1320aaaagtttta taagagtttt gagagaggtc gacctacctt ccaacggttc cgcggaaaag 1380ttttataaga gttttgagag aggtcgacct accttccaac ggttccgcgg aaaagtttta 1440taagagtttt gagagaggtc gacctactct ccgaggttcc gcggaaagtt ttataaaaag 1500tttttacaaa gtttttgagg gaggtcgaca cctcggatcc tccgcctcgc gtgccgcggg 1560cggcctccaa gagttttacg gaacgttctg gagaggagag gccgacctcc caaagatttt 1620gcggaacgtt ttggagagga ggactggcct cgaaaagatt ttacaaagag tttggaggga 1680ggactggcct ccaacggttc cgcgaaaaag ttttgcaaga gtttggagag aggtcgacca 1740ccctccgagg ttccgcgaaa gtgtttgcgg agagtccgag agaggcagac actgcctgcg 1800aaaagttttc gcggacggtt gagcgggtca ccgacctccg acagtttaca aacgttttac 1860ggagagttag agaggcagca gaccgacctc cgaacagttt agttttacaa agttttggag 1920ggtgaactgt catcgggaaa gttttacgaa gagttttggg agaggccgac ggttagccga 1980gcgagcgcgc gagcgagttt acgttctctc gctcgtgtgc gcgttaactc gcactggttc 2040cctctctaac tcggagtggg cgagcgagtg gttgactcgt cctccgctct cactctgagt 2100ggtgataact gttaaccatt aactcgtcct ccactctctc actctctcac tctgagtgag 2160gattgacttg ttaactcgtc ctccactctc tcgctctctc gctctgagtg agtgaggatt 2220aactgttcaa ctcgtcaact cgtcctctgc ctctcatcca agactgagtg ggtagttgac 2280tgctcttgtt ctcttactcc gaggggtgat tgagttagca acagctactc gttctcttgt 2340tctctcacac cgagtgagcg agtgagcaac gttacttgtt ctctcacacc gagtgagcga 2400gtgagcaacg gttaactcgt tctctcgttc tctcgttcta ttattccgag gagtggcgag 2460tgaggtaacg gttactgtta cttgttctct gttcccttcc tcggtgagcg gtgcgtcaac 2520ttgttctcgt gagtgagtac ggtcacttgt tctctcgttc tctacctcga ggagtgagtc 2580aacttgttct cgtgagtgag ttgaccgtaa ccgttccctt acctcaagtg agtggcggtc 2640gcttgttctc gtgagtgggt taaccgcgtt cccttaccgg agtgagcggg cgggcgataa 2700aaataatcaa ttgactgatt cgctcgtgag cgagcgaagg gcggcggaca agggcgcggg 2760atgctggtct aatctactaa ggccgattac aaaaacggat gggagaccgg gagggagagg 2820gtcacagctc cgagcggtgc atccgcgcca gctggcggcg ccactcggcc gcgggccgcg 2880gccgcccagg ccgcgttgta gccgcccgcc accgcgacgc aggtcagctc gaactccggc 2940ccgagcgcgc gcacgtcgta gatgtgcacg gtcgcgacgt tcagcagcag cgcgcccttg 3000cgcagcgagg cgaaggtcgc gtgcatgccg gcggcgagcg ggtacaccgg cgagagcgtc 3060ccgccgccgt gcgcgaccac cgccatgtgc cgcccgtcgc cgccgacggt caggcaggtc 3120accgaggcgg agccgttcgc gcggacgggg cccgcctcca cgagggcggc cggcagcggc 3180ggcgccgcgg ggcggaagag cccgccgagg aggaagccca gcgccaccag cgcgagcgcg 3240ccgagcagcc tgcgcggcga ggggtgcatg cttgttggct gcggtgttgg cgtctggcgg 3300gtggaaggcg ggtgtggggt gtccgcggct gcggcgggag ggtctcgact gctaggcggt 3360cctttttcac tttgctccgt ggcgcctggt ccggggcaag ggctgcggcg ggcgcccggg 3420cgggcgggcc gctaccccgc cgcgcggccc gcggccgcca gcgccgcggc cagccgccgc 3480caccgcgggc ccgccgcgcg cagcagcccc gccgccgagc gccccgcgcc gccgcgggcg 3540cgcgccgagg ccgcggcccc gcgccgcgcc agcagcagcg gcagccgcgc gtccagcggg 3600ccgccgcggc gcagcgccgc gcgcagcagc gccgccagcg gcagccgccc cgccgcgtcc 3660gggcccgccg cgcgcgcgcc cgccgccagc agcgcgcgca ccagcgccgg cgagggcgcc 3720gcgcgcggac caggtgctcc acgagcaggg tggtgagcaa ggattctcga gaagtaggag 3780tcatgtgtga cgacaaggag agacgttata ttaggcgcgt cctacttcac tttgaagatg 3840gtgtaaagtg ttaaaacttg aacaccgttc actccaccac tgccgttacc gtgtcctgcc 3900ccaaaagcaa ccacagtgct ttttccacca cctgttccaa atccgttcca aaagctccca 3960tccattgttg ttagaacttt cagatgtttc tctaggttgt ttagttccac tgcaagtttc 4020gaccattatc gttactggac atgctgttgg taatgagttt aataaccaat cataaaaata 4080gttataattt gttataaagc taataaagta gcaaacactt taatgttata ttttgcctaa 4140ccctccgtta acaccaccat taacaccacc acttaagctt ttactaccac cactaccacc 4200tccaacacac attcttttct ctaaaggtcc ccaaattcca cctcctgaac ttggacgttt 4260tacagcacct ccgggtgtac ttgcgtaccc tttagaagtt ccactgtgac tgtagatatg 4320atactgtcct tctccaggca tgattaaagt gtgttgtaat tagtgttatc tacgcaactg 4380tgcgagactc tcgaataaaa agaagctaca ttttacaatt ttgattagct gatgtaccac 4440gctgtatcgc ggccaccaca agcacccgat ccagtagaac caaatccaga gtcgccgcgg 4500tcagtgttgt ccaagcagtt aacctcttga actgctgggc acgatatgcg ttcgcatatt 4560agctgagcta tcctgtctcc cttcttaacc tcaaagtcac tgtttccaaa gttaaacagc 4620accactccga cgttgcctcg gtagtcttcg tcgatcacgc cagcgcccac gtcgataaag 4680tgtttgactg caaggccaga acgtggtgct atgcgtccgt agcaaccaga agggggcttt 4740atcagaaggt cagtaaatac tacgcgactg caatgcgaag ggatgacaca gtcgtatgca 4800ctacataggt ctaatcctgc ggcaccagga gatcctctgg ctggtatagt ggcgttttgg 4860ctgaggcgaa caacctgaag agtttccgtg tggcagaact ccatggctag ggtggcgagc 4920ggccgatcga ctacggggtg tacaatttac actttctcca gaaaaatcag gggcgggtca 4980gcatggcgcg gcgcaagtcc agcagcgagt cgtacgacag gaagcacagg atggaggtca 5040cgatctccgg cggcagggcg cacgggcaca tgaggccggc gatctgctcg gccagcgaga 5100cgcgcagccg catcatgcag atcttgccga agagcgccgt cccgtagatg gggaactcgg 5160ccgcgcgctc caaaaaggcg ttctgcacga agagcgcctt cgcgtcgtcc gacgcgcgca 5220gcacgtccaa cagcgtcgcg tccgtgtggc agcgcaccgc gcgcatgctt gcgatctcct 5280gctcgcacgc gcggatcacg gtcgcgtagt ccgccagcgc gcgctccgcc agcatgcgcg 5340cgccctcgcc gcgcagcgcc agctcctgca cgcacagcag cgcggcctcc gagcgtcgga 5400acacgtggcc ccattgctcg gatgtgatca gcgcgcgcgc gagcagctcc gtcggcggcc 5460ggcgcgcgag cacggccgcc gtcgcgcgca cgttgttgcg gcgcagcatc tccgaaaccg 5520cgcataggcc cgaggccgac atgtgctcga gctccgcgcc catgcgcacc agccggcagc 5580aggcgccgtg gctgaacacc gccgcgcggt gcagcgcggt ctgcaggttg ttgttgcgca 5640ggttcaggtc cagcccgcgc tcgagcacga agtccacgac gccgcgctcg cagctcccgt 5700aggtcgccat gtagtgcagc atggtgttcc cacacgcgtc tacggcggcc gggtccacgc 5760ctaggcccgt gagcgtgcgc accatgccct cggagatctt ggccgtgcgc gcgaggtggt 5820gcagcgttgt gcgcccgtac gcgtccacga cgcacgcgtc cgcgcccgcg cgcagcatca 5880tgtccacgag cgcggcggag acgccgccgg agcacagcag cgccgccagc ggcgtcaagc 5940cgttgcagtc gcaggcgttt gggttcgcgc cgcgctcgag cagcagccgc agcacgtcct 6000cgcggatcca ctggttcttg gcgtacacgt gcagcggcgt tacgccgtag gtgttgccct 6060cgttcacgcg cgcgcccgcg tccagcagca gccgcgcgac ctcgagctcg gcgccgtcgg 6120ggccgcagaa agccaggaag gaggagagca cgctgtcgca gacaacgacg ctggcgtcgc 6180agaccacgtc cgcgcccgcc tccagcatga gcgcgaccac ctccggccgc acgccgtcgt 6240actgcacgta ggcgtgcagc ggcgtgcggc cgcaggagtc cttggctttc acgtccgcac 6300cggcctccag cagcacgcgc acgatctccg cgcactgctc gtgccgcgcg aagtgcacgc 6360agaggtgcag cggcgtgcgc ccgtgctcgc cgcggaagtt cacgtctgcg tcggtggcta 6420cgagcgcgcg gaccgtttcg aggtccacct gcccggactc caggtagcgg aagagcaggt 6480ccgcgtgcgg gaccacgacg gactcccgcg agagcatggc ggcgtttaca aatattgaaa 6540tcttttttca ctcatcttta tggcgctgaa cgcgcaataa gggtgagagt aaaaaacttc 6600tacaaaaagc gtacaaaagg tacaaaaggt aaaaaaggcg gggcggggac gggctggggt 6660gctgcgagct gaattggcct ctacacaggg acgccctcgc cggagccggt gagccggtag 6720ccggcgccgg cgatcatggt caagcgctgc acgagctcgt tgcgcttgac gccggcctct 6780gaaacgcaca ccatgtggtg gatgtaccgc tcgatgcact cgcagcgcgg gagagtggag 6840tcaagatcgg atgcgagttg cagaatgtca tcccagagct cggagaactt gctgtacagt 6900tctcggaggt ctctctccat tcgagccgta agagagtcag gatgcggtgt tccttcggga 6960gtctgagcga acaccgcgaa caggctggtt atgccgtgtt ctagaataga gtggttccgc 7020gttaatgccg cagacaaggg tcgtcgtccg cgcaacgact ggcggcagag cgctgtttgt 7080gccgcaccgc ccattcctct ggcgatcgca tccaccgacg cagtgatcat ctgcgcgccg 7140acgtcattgt agcgcgcgtt aaactcagta atcatgatta cgagattgca gatttcatag 7200tagcactttt ccaagtcgac gcgcagtttc acgatctggt tgacaatctt gcacgccttt 7260cgccgcgtct ccgccacgtt ggcgactcgg acttgcgctt cctggtcgat ggacggcgga 7320aacacttcaa acccaaggtc gcacagttca gcggtgggga ctagcgtcac gatgatgtac 7380tccgcatcgc cacccacttg cggcaggaag aacaccgacc gcgcggcggg aacgaccaga 7440acgtcgcctt cctgcatgtt ggtttttaga aacttagtgt tgttcacgga gatgccggcc 7500atgccctcgt ttttgacaca tattatggtg acgtacgcgg cgaccgtggg ggccatgtgg 7560tggcgcatgt accactcgtc gtgcttgagt ttcagaccgt gagattcgcc aacctcgaag 7620tgcatgttgg cgtctctgac gtagcgcgag aactcgctgc gacagattcg cgcgggcgcc 7680cggtggaacg tcgactcgaa gagactgatg gctgtccatt cgcccacatg agtgaccacc 7740gaagaagtgt tttcgatccg agtctcgaac accgagtcca cgagcaccgg acagttggtt 7800ccgggcaccg tcagcaccaa gggccgcgcc tccacggggg cgacggacga agccacggag 7860tcggtgtccc cgtacccgta gtcgtcgtcg gagtcgccgc ctccgtcggc cccgtcgcgc 7920ggcctccgca gcggcatgca gccggcggtg ggaacgcact ggtttcggcc acggccgaag 7980cggccaaaca gtctcgccag ggctgacatc cttggacggc cacaccaaaa ccaaaaaaac 8040atattttatc agttatttgt cgattttcac cggctcaccg agggcaggac ctcctggatc 8100ccggacaccc ccgccaggca gcgggccgcg cgctcgcgca cccaaaagcg gtcgtagccg 8160tgccggagca cgaaggccgc cgtggcgtgg cagtccacgc gctcgatgaa gccgtggacg 8220gcgcggcgcg cgtagctcgc cgcgaaggcg cggaccaccg ccgagcagcg ccccgagggc 8280gagtcgtccg tctccagcgc cagcggcatg ctcgcgatgc gcgacatcag gttggaggtc 8340tgcgggatgt tgagctcgcg cgtggcggtc atctgcgcct cgagcccggc cttgagcacc 8400tcgtcgcagc ggccccactc cagcgcgcag accacgcgga tctcgtaccc cttgagccgc 8460agcgcggtct cgatgtccac ggaggtgagc accgcgctga agcgcaggcg ctcttcgtcc 8520gcggggtcga agagcacggg gatcttaacc tccgcgctgc gcgtgacctc gcagagcgcg 8580atcgcgagca gcccgcgcgt gagcttgctc accatgcgcg gcttgcccac ggggtacagc 8640tggctcgcga cctcgcgcag cgggtacgcc agtcggaagc agcgcgcgtc cgcgggcacc 8700gggctcgcgc ccgtctcctc gaggaagagc gcggcctcaa ccatgttcag cgcggagaag 8760tgcaccgggc aggcggcgca gccgcgcgcg gcgttcgcga gcaccatctc gcgcagcccg 8820cggaaggccg ccatgtcgca ggaggggaag atgcgcgcga gcgcggcctg gtgcgcgagc 8880gccgcgtccg agagcgcctg cgcgccgcgg cggcgctcct cggcggcggc cgcgctctcg 8940tccgcggaga ccacgtcttc gggcacgtcc acgcagacgc cgccccagaa ctcgcagtac 9000tcggagaaga gcgtcgcggg cgcaaagcgc gcgaggtcca cgaaggcgac gcggttgccg 9060agcctggaga gcagcgtgtt ctccgagatg cgcgtccagc ccttgccggc gagctccatg 9120acctgccgcg tgtcgaagag ggagctgtag aagccgtaca cggtgatgtt ttccttgcac 9180gtcgtcagcc acatgaggaa gtcgcgcacc accagcttcg cgcagtctcc ggagaacacg 9240gggccggcgt tcgtcgcgat ggagttcagg cgcacggtgc cgtcactgcc gaagcggtac 9300acgaaccagg cggccacgct gttgccagag ggcgtgtgaa cgtgtggctg cgcccaggag 9360tcggcgctcg cggcggtgcg cacgtcgtgc gagagcacct cggtgtcggg gcgcgagtag 9420gtgctggggt ctttgatcca gatggcgtag ctgcccacgc agcacacgtt catgaggtcg 9480agcagcgtct gccggcgcag cggcgtgccg agccggcgca cggcgtcgtg cgagaccatg 9540cgcaggtcgt agaggcccac gtccgagagc cactggttga gctcgtccat ggacagggcg 9600tcgcgggggg gcgggctgtc ttcgaaggcg gcgcggagct cgggctccgt ctccgcgcgc 9660tgccgcagga tgtccaggaa ggggctggag gagtcgggga tgtagcagtc ggggtcgtgc 9720ctggacacta tagcgaaccg ctgcgtcgcg ggcggcgggg ctagcgcgtc ggcgcgtgcg 9780tcgatgaagg tgcacgatat acgcacggac ttgagcgagg ggaggacgac tgcggcggcg 9840cgcgcgccct ccgcgtcgaa gatcatcgtc tttccgtccc tcgcctttgc gagcgcgtat 9900tctccaggca cgaggtccgt cggcggcggc tcgtcccagg cctgccggtc agggacgccg 9960ccgcacacct ttccccagaa ccccagcatc ctccaaaata cctataagga cggccaatag 10020cggggcttgc gggcgttcgg accttccgcg ctttaatttt aatttattgg cttgcagaac 10080tccgagcgcc agtcccgctc gaagaccgcg gacaggtcct tgacgatgtc gcccttctcg 10140gcgttcacgc tcacgaaggc gtggtagcgg tagtgcgtgc cgtcgaggtt ggcgaccgtg 10200aggtgcgcga aggtgtcgtc cacgatgagc agcttagtgt tgttcgcggc gtcgtcccgg 10260ccgggtacca cgaacttgcg cacggacatg tccacgctgc cgacgccaaa gtcgtcgagg 10320ctgcgcgcgg ccgagaccga aagcgggtcc gcgttcttcc actcggtaat gatcacgcgc 10380acgcgcacgc cgcggttgat ggccgcgcgc agcagcgcgt caatgatctg cggccagtac 10440tccacggcgc tggcgtgctt gatcaccggc accatcgaga gcagcgagag gtcgatgctg 10500ttcttggcgt tctcgatgcg gtgcagcacg aggtcctcgt cgagcgtgcg gtagaagcct 10560aggaagcgct ccggcgagtc cgagaagaat acgccgcccc cggagtggtc gaggtggaag 10620ttcgtggccg tgggcgtgac gatggcgcag cagagccgcg tgaacggcac cttcggctcc 10680acgatcatgg agtagaaggt gttgtagcgg ttcatgaggt cccaggccag gtgcttgttg 10740gtggagtaga gcccgaggtt cttgatggtg gacacggacc cgcccgtgag cgaggcgctt 10800cccacgtacc agtgcccggc gtccgagagc cagaagctgc cgagaaggtt gccgacgccc 10860tccttggtgg acaccttgac cttgtagtag ttgacgcccg cctcgcgcag ctcgtccgcg 10920tccttgtcct tgctctgcac gtccacgagc agcgtgacgt ctacgccctc cttggcgagc 10980gtgcagagct tgtccttgac gtcgacgccc tccttggtgg agctcaggtt gcagcagaag 11040ctgcagatgt acaagaactt cttcgcggac tcggcgatag cggtgaagca gtcgagggtg 11100ctcatgttgc cctgcgccaa agacgccacc tctgcgggca gcgtctccac gacgcggcag 11160tcggcgccca gggggatgga ggagaacggc cacatttatt tatctcacaa aaataatagg 11220gcttcaggga aagtctttta gcaggcgggc gagttcttcg agttcgctta ggagttcttc 11280catttcttcg gaagtcagca actggagctc ggacttgatt tgaatatctt cgaggaaacc 11340gtctagcatg ttcgccatgt cttccgggga gcactgcgcc acatcttcgg ggacaggatc 11400gggtgtgggc attaggtctc cgcttacttg aacgtcgtcc atcatcctgt cgatgaggtc 11460ttcgacttct agacggggtc cgtagatcag catatttggt gatggaggta gtttaaggtg 11520cgagagttag tgttatacga ccgccaacgt gtgtttatcg cgcgtacatt ttcaataatt 11580aacaaactcc ccttcctgcg cctgctcgag aagcagctcg tccagctcct cctgtcggcg 11640cgcggccacg cgtctttccg cgaagagtac catcagctcc agccccacgc cgcacagacc 11700caggacgccg aacaccaccg ccgccgagat cgacagaccc agcagcaccg acatcctcac 11760gcgggcatcc ggctatttaa tcgttctgga aacgtattaa tatgggcgtc gtcatgtgcg 11820ggtgtctgtt tgtgtgggcg ggctggatcg cgcgccgcgt gcgcggcctc tgcgcggcgc 11880tgcgccagag ggtgtcgcgc gacaagggct acgtggccgt catccagacc tgcgacgacg 11940actacttcac agaggaggag ttcgacgacg gcaagcaggt ggtcgcgctc ctgcgcgacg 12000tctcgcgcgt ggttgccgcg cccgcgggcg tgacggaata agttaggata aggagtcgag 12060gggagaaaaa cagcggtcac actataaact cgcgcgaggc cgattttgac gtgctcatgt 12120ctggaagctc cgctttctgc agcgcggagc ggcacacgaa gcacacttcc gtgttggtgg 12180gagttatgca gtggacgtgg tagccgtgcc cgcacaccat gacttggaac ggacacgcgc 12240cgggacaggc cgcgtttatg catccttccg gcgagcgctt gttgcagatg tagcacacgt 12300ctgagcacgc cagcgtgcag gacacggcca gcttccactg cttaacctta acgggcatgg 12360ctagttgaac acgaccatgg gcgagtcgcg agcctcgagt cgggggttca gggcaaaccg 12420tttcacgccg tcaacggttc ttctctttgc aattttctct ctgcacaggc tcgtcagcgt 12480catctcggcc aggcgcgcgt cgttgcctag gtgccgcgcg gcgtcctcga ccgtcacgcc 12540cgtcttgccg gcctcgtcca tgagcacaat gcataccagg tgcgcgctag agcatatgac 12600ctcctgctcg cgcccgccgg cagcggggat ggttagctcc gcgcgcccga aggccgccag 12660cggcgccacg tcgtaggcag tgtctgctcg ggcgagcgcc gactccacgg caccgcggag 12720cgactccggc ggcgtcagcg cggccagcgg caccggcgtg ggcacggtgt acacgttcac 12780gggcatgagc accatctccg ggtcgtggtg gccgctctct tcgccgtcgt gctccatggg 12840ctgcggcggc ggcagcagcg ggagcagcag ccgtccggac atgagccggc gcacaaggtc 12900gttgagcgcg gacgaggcca tcggcgggta cagctccatg gccagcttca gcgataggtg 12960cttctcgagg ttgacgccgg tgtagacgct cttcacgatg cgcgcgaagg ccacgcgcgc 13020gaaggccgcc agctcctcgc gcggcaggcg ctcgatgtag gagagcagca tgtcggtgtc 13080gcacggcggc gccgcgacca ccgcgccgta gagcgccttg cccgagagct tttccagcgc 13140ccttgcgtgc aggccgtggg tcttgagcac gtccacgtag ttcacgtaca ggcagagcgc 13200gcgatcgagg ttgctctccg cgacgtgcgt ctcgatgcac tccacgatga gcgggcccat 13260gcggtccttg atgaggtcta tgagcccgcc gtaggcgact cgcgcgctca tgaggcacgt 13320gcggcagtac gccatcaggc cctcgaggtc cgcggctatc acgtcctcga ccacgttcgc 13380cacgacgcgc tgccagagcc gcaccttgct cacgttctgg tgccgcacca tgtccacgag 13440ctcgtcgtac gagccgccgg gctcgtgcgc gcgatcgacg atgcacctcg ccatggtgcg 13500gctctggcgc atgagctcgt tcgtgaagcg cacgcacgcg tcctcggaga agagcgcgct 13560caggcaggag tagcagcggt ccgcgacgag gtgcgggaag cggcactcca cgacgccgcg 13620gccgatccgc agcacgcact cgccgtacat ctcgtccatg gcctcgcgca gacagtcgtc 13680cagcacgtcc gcgttgtgcg cccactggat cacgcagagg tagggctcga tgttctcgcg 13740cgcgttttcc acctcctgca ccatgtactc gagcacggtc atgtcctcgt ggatgtcggt 13800gcccagcatg cgcccgggcg gcagccagct cttgcgcgcg atcgcctctc gcaggcacgc 13860caccgccgtg aaggtgttga cgcggagctt ggtcagcagc cgccgcagtc gggagatgtg 13920tgccacggag aggtccatct ccatggcctg ggcgatgagg cgcgtgagtt cctcctccat 13980ggcggcggct ccgcgggcag atatacgcga acaacggtaa gccgtgctat ttcatttttg 14040gacaaaaagc tagtcgtcga cgcgcatgtt gtcgaggttc cggcacagcg agagcacgtc 14100gtcgcgcgcg cgcctccggc gcagttgatt gttcgcgcgc cgcgcgtccg cgagcgcctg 14160tctgtacatc gcggagtccg cgtacccgtg cagcggcgag cgccgagcgc cgggcctcgg 14220gctcgcgcgg cgcgggagcg gcgttggcgc gcgcctcgag cgccgcgcga agtgcgcctg 14280catggccagc aggcaaccga acggcaccat gtatcggtcc atgaggcact ggctggccgc 14340ggacggctcg cgcgggtgca tcacgccgcc gcccacgtcc tccatgacgt cgcgcagcac 14400gcagcgcagc atggtctcca tgctgcgctg cgtgaaccgc accggggagg cgtcgacgta 14460gaagccgtcg gccacgaagt agagcgcgtc cagcccgccg agtttctcgc cgagaccgac 14520gaagagctcg tccacgtgcc agtccaccac cgaggccttg aagagcacca cgtgccggat 14580gtcgtgcgag cgcgcgagct cccaggtgtc ctcgccgatg ttgctggcgt cgatgcggcc 14640tgtcatgcgc acgctcacgc acggcgtcat cccgttcttg tagcagaact ggcgcgcgag 14700ctcctcctgg cgtacgatgt cgaccatgct ctccatgaag gaggtggaga gcagcatcgc 14760gccgcgcgcg gcgcgggtcg cgttttcgtc cacctccact tccatcccgc cgccgatcct 14820aatcatctat cgtatttaaa ttttcggcgg agcagacacg cggctgctcg ctgcgcgatc 14880gcttcagccg cggcggcgtc acgcacgcgt tgcggcggcc ggcacgcacg gacgaccgcc 14940ggggctgttc gctgagcgag cgccgcggcc gcgtgacgcg acagtcgcag gtgggttgcc

15000gggagtcgct cgcgcgcctt cttcgcattt cgccggaacg ccgcgtttat gtaggggatt 15060atattttcaa cgtaactaaa tggacggggg cgtgcacaaa cggcctttca tcgtgaacgt 15120ggatggcatg ggcaaggtgc tcgtgctccg gtacttgcgg atgtgcgagg tgcccgaggc 15180caagtgcgag gggtcgcgcg cgtcctgcgt gctcaagatg gaccctcccc gctcacccag 15240ctgcgagcgc aggccgtctc tcccgccgtc ccccccatgc cccatgcgca cgcctcccgg 15300gtcgccgctc caggctccct tgatgcgcac gcagatgctc caggggctgt tcgacgctgc 15360caaaaacaac ggcgagcaga tgtgccgccg ccagtaacct aggctgcgca gtacgaaagt 15420tagtgcgtga tcacgttttt tgcaatgtcg atcacgccgt gcgtgcccgt cttgcgctcg 15480cgctccacca cgctagtcac gggccgcgcg tccgagacta gcgaccccag catcgagcgc 15540acggcgccct ccgcggcggg gtggcgcgtc agcagcagga acatcacgat gtgcgcggag 15600acgccgcgcc ggctcagatc gtgcacggcg tcgccgtcca tgagcacggt gttcgagaag 15660tacgtgaaca gagtgttgtc tcgcaccagg aaggccgagt tcgagacgct ctcgaagtcc 15720acgatctcgt cgtcctgcac gcccatgtcc aacagcgtct gcacgagcgc gggctcgtcc 15780aggaacacca ctgcgcgcgc gaacccgcag tccagcgcgc gcgcgtccgc ctccaacacg 15840cgcgaggcgc cgccctccgg cggcaggaag gcgcagggca gcggcgtgcg tccgtccgcc 15900ggcgcctccc cgagctcctc gagcgcgaag gccagcagcg tctccatgcg cgcgcgcgcc 15960ttgtcgaagt tgtccgcgag gtcgcggatg cggtctgtct gcgagaacat cttcagcatc 16020gccatgagct gcacgaaggg gtgcagcacg tatatgttgt ccacgagcag cgtgggcagc 16080gcgcgcagcg tggcctgccg cacgttgaag ctgtccagga tgtgcccgcc ctcctcgtcc 16140tgcagcacca cgtagttctt caggtagggc acgcgcagca gcacggtctg ccgccccgtg 16200acgaagtata tcaggaaggc gaggttgatc aggaacgggc gcgcgttcgt ctgcaccatg 16260tcgatgtcgc cgtactctat ctcggggttc agcaggtgca gggcgtacga gccgtagcac 16320acgcaccgct tgttgtgtcg gcgcaggtgc tccttcacga gccgcttgac cacctccacc 16380aggtccgagt gcttgtgccg cgccatcggc gcggcctcct cgggcggcgg cagcactgcg 16440tacgagttga gcgcgcggct ggcgagcgcg cgcgcgcggg gcgcgtccac gcgccgcacc 16500gccgcgttga ttgcaggcgt cggcgtcgtg agagagccca gcgtgcgcgt gaactcgctc 16560acgatcacgc tctgcagctc cagcaccgtc aggatctggc ccagcttctc caggcgccgc 16620tgcctcgaga agtactcctc gatgcgcgcg gcgatctcct tctcggagcc gcctagcttc 16680ttgaagaagc gacgacgact ctttacaaca agagagagaa aaagcttcct atcgaagttg 16740aggacgcggg tcatgttgcg gcgctgcgcg cgcaggagca cgcagcgctc catggagggg 16800cgcgagccga ggtactcttc gatcacgggt ggagccatga cagctatttt ctgaacccgc 16860gattattgta cagcgcaagc cgcgcgcaga cctgctggca cagcagcgtc gtgtttcgca 16920tgcacacgcg cgaggactcg atcgtgcgcg cgtccggtgc ccaggcgcgc agctccatca 16980gttcctgctc gacgaagtcc acgggctcca cgaagcgctc tgcgcagagt ccgtccgtga 17040acgcgttgac gatctgccgc acgagcacta ccacgtccac ctgctccacg aggcgcacgc 17100ccatggcgac gtgcacgaag aggcagcgga agagcgcgtc catggccatc tggtggtccg 17160agcagggccc gaccgcggtc ttgcagccca gcgcgaagcg cccgatgccg cggtactgca 17220ccatctccga gggcgagaag gagagccgct ccatcttgag cacgggcggc gggcccgccg 17280gcagtccgcg cgcgaggtcc agcaccggcg tccacccggg cgtgaacatg tccgggatca 17340ggaagagccc gtagctggcc atgcgcgcga tgtcgaaggc gtggtccacg accttgttca 17400cggcgctgtc cgcgcggttt acgcgcagcg cctgcaggat cacgtttccg gaagcgtgcc 17460gcgtgatcgc gaggtccgcg gtcgcgtacc cgcgcaggcc cggcaccgcg tacgcggtca 17520gacacacggc caggcgcgcg ctgtgctccg aggagaagat ctcctgctcg tagccctcct 17580cgggctcctc gcactcgcga gggcgccgca cgtcctcgac cgagcgcagc cgcatctctc 17640cctccgacac cagacagcca agcgactccc tcaccgccgg cgcgagcacc tccgtggcgc 17700agagcgcgtc gtgcacgcgc ttgagtgtgt tcggcttcag cgcgtagccg aagagcagcc 17760gcgtcatccg cgagcccgag aacgcgaagc ggcgcacgta ctcctccgcg agctcggggc 17820ggtcgttgat ccacgaggta gagaagacgt ggtcggaggc gaaggggtct gcaccaaccg 17880cgagcagtgt ggacagaggt acggtgtcga ggaagtccac gacgtcgggg aagttctggc 17940gcacgcaggc ctcggcgacg cgtctggtgt gcacgcacat gtcggtgacg ggcacccggt 18000ggccggactc cacgacggac acgcagacgt cctcggtgac ggcgtccacg ggcatggtcc 18060gcagcagctt gccgagcacg tcgccgaacc cgccctcgag cgccttgcgc cacacgaacc 18120ccgcgtcgaa cttcccgggg aagtccgcga tcaccgaaag ctccgcgtgc gagaggttgt 18180ccacgttgag gtaggtggcg gcgtccacga agatgggccc gaaggcgccg gtgtcggaga 18240cgcggtctct gaggtagtcc ctggcgtagt ggaggtactc ccgcacctgg ccggcgcgga 18300tgcgctcgag cgcgaaggcc ttcatggtct cggagcagag cacggagtgc cgcagcccgt 18360ccagtgtgcg ccgcacgtcg tagacgccgc gcatctgcgc gagcatctcg acggcgtccg 18420ccggcgtcgc cgccgcgagc cccgggttca ctggaggtat cctgtgttct gcgagcatgc 18480gcttgaggaa acagaggtcc agcggccgcg tggtgtacag cgcggaggcc atctcggggc 18540gcgtctcgac gatgtcctcg atcatctcgt ccgtgaatgc cgcgttgatg ttgtgcacgc 18600tgcgcgcgtt cacgtgcagg aggatgtcgc ccacgttgtc ggggaatcgc tcctcgatga 18660gccggacgtc gtcctccgtg atgttcatgt agggaataca gcggcagagc agcgcgtagt 18720ccgcgaactg cgcgatgtag ggcgtgtgga actcgatgtg tctggcgaag agcgcgccgc 18780agcgccgccg cgagagctct tcgagcaggt cctcgggcgt gacgtgctgc gggcggaaga 18840ggtgcaggtg cgtggggtgc tcggcggcca cgcgcgcgta cagccgccgc gggaggtgtc 18900tggggtgcac gccggcgagc acgagatcca tggcctctga ggtagacagt gcggcgaacg 18960cgcgctcggt gccgcccgcg gcgacagcgg cgccgacaaa tctcttgagc agctgcagca 19020tcgcgtgttt gggctttcgc ggaaggcgct tattttaatg ttattggcgg tggccggtgc 19080gagataaaaa ttagaactga tgccgcagtt gttgatgata tgatgattgc gctggccggc 19140gcgagataaa aattagaagc tgatgccgca gttgttgatg aggatggtga gtgcgctgga 19200gcaagcggtg tggcgcgcta gcttcttgct ggccccgtcg gccacggcaa cgacctttcc 19260ggatatcgtg atggtgcagg tgaagcgcgg acagtgatcc tctccgccag cacgcgtctc 19320gcagaactcc agaggtctgt gtgtcatcat gcagaactcg ttgaccgcgc tgaccgggtt 19380aagacttttg aggcgtatca cggcagactg agtcatgatg tcgatgtcgc cgccgaaaag 19440cgtatcgcac ccagcctcgg tctccatggg ctcggtgtcg gagttttcgt cctcctcggt 19500gggcgcggag ggcgcgcact ctacgaacca gcggggcggg tttccgtcct cacagcaaac 19560ctcgtccgag tccagcaggc ggtacagctg gcggtttgcc tcgtgtttgg atatgccgag 19620ctccttcgcg atttgcttgg ccggcagctt gtcgtcggat tttctgagaa gctcgaggat 19680cagagacgcg cactcgcagg ccattgtggc gatttacggg gcgtgcgttt ttttaggatt 19740ttggcttgcc tttcttttcg cagaacttgg gaggattgaa actcttttgg caatttttgc 19800aggcgtactt gatcaagggc ggctcgtccg ccgagcgcgt ctggatcatc atcggcatgg 19860tgttcttgct ctggcacgag gggcagggca ggttgaactt ctcgtcgagc acgttgaagt 19920acccgctgta gtcgtggtcc ggcacctcct cgatgtcgta gggcacgcgc gcggccacgc 19980acttgaccgc gaagagcagg taccgcagcg cgtcgtgctc cgcgccgctg gtcgcgcgga 20040tctgcgcgca caggtccgcg tagtcctcgt tcgcgtccac ctccaggctg cgcttgttct 20100tgtacgagag ccggttcttg gcgtccttcg agtactcgat gccgatgttg tgcgcggggt 20160caaagttggt ctcgtcggtg ttcgaggtct tggtgttcac gatgttcttc agcgcgaagc 20220gctgcgcgca gtccagcgcc catcgcgcga tgcgcgcggc ctccgccgcg tcggtgtgct 20280tcgccgcgag gtcgcgcagc cggtcttcgt ccatcgcccg attttaggtt gggtatatta 20340tctcaattcc gctcttccgc gggccgcggg cgcgcgcccg cggcaaatta ggcgttacaa 20400atggacttcg tgcggcggaa gtacatgata cacgccatcg accgcaacct cgacttcatg 20460aaggccgagg tccagcagaa ggtctccatc ttctccctcg ggcacgtgct cgcgctccac 20520tacctggtca ccgcctttcc gcaggcggtc atcaccaagg acgtgctcgc gagcacaaac 20580ttcttcgtgt tcgtgcacat gtcgcagcgg cacgaggtct tcgacgccgt gctcaaggcg 20640gccttcgacg cgcctcagct ctttgtgcgg gcgctctcgc ggcacttcga ggccttcgtt 20700gccgccatcc gggcctaccg cgcgacctgc gcggagctgc tggccgacgc gcgcttcatg 20760gaggtggctg cgcgcgcggc cgagctcgcg gaggtcattg gcgtgaacca cgacatcgcc 20820gcgaacccgc tcttcgcgga cggcgagccc gtgcgcgacg cggagctcat tttcgcaaag 20880accttccgca agaccgagtt ccgcgccgtc aagcgcctcg ccgtgctgcg gctgctggtc 20940tgggccttcc tcgtgaagaa ggaccttggc ggcgagtacg cggacaacga ccgccaggac 21000ctgtttacgc tgctgcagaa ggccgcgggg cccgtgcgcc acagcgcgct cacagagagc 21060atccgcgagt acctcttccc cggagacagg cccagccact gggtctggct gaacgcgcgc 21120gtggccgacg acgcagaggt gtaccgcgac cggcccgcgc gcacgctcta cgagcgcgtg 21180ctcagctacg cgtactcaga ggtcaagcag gggcgcgtga acgccaacac gctcaagctc 21240gtgtaccggc tcgaggacga ccccgacatc aagggtctgc tgctgcagct catctacgac 21300gtgcccgcgg acatcgtcgg cgtcgtggac tccgcgaacg aggagtggcg gagctacttc 21360gtgagtctgt accgcgagaa cttcgtcgac ggacgcacct tcacctcgga cgcgcgcttc 21420cgcgacgacc tcttccgcgt ggtcgccgcc gtcgatcccg acttcttcga gcccgagcgc 21480atccgcgagg ccttcagcgc agacgcgcgg ctgcgagagc gcttcacgga catggacctc 21540aacaacgcct tcatgtcgca cctcatctac gactccgtgg accccgacgt cgccgccgcc 21600gagcgcgggc tcgcactgcg cgtgcacaac gaggactccg actacttcat ccgggagtac 21660aacacctacc tcttcctcag cgagaaggac ccgctggtgc tggaccgcgg ggcgctcacg 21720cggctctcgg acgtccccgc cgagcgcttc cgcgacctct tcagcgacag tgtgctgcgg 21780tacttcctgg acgcgaagct gggcacgctc gggctggtgc tcgaggacta ccgcgaggac 21840gtggtcgccg ccatgcttcg gcacctgcgc cgcgtcgagg acgtgtcttc cttcgtgacg 21900tacgccgcgc gcaagaaccc cgcctgcgtt cccggcgtcg tgcgcgcggt cgtgagcaac 21960ttcaaccccg cggtggtcgc ggccatgcgc cccttcctgc gcgagcacat gacgcgcgtg 22020gacgcgctgc tggacggaat gccgcacctc tcggaggccg accgtcggta catccgccgc 22080gtggtgctgc agggccgcgc ctgattcgcc gtcaataaat cgcgatggtg gacagcggca 22140cgcacgacgt ggactccgcc gcgcaggagc gcacgcccaa ccagcagacc ttcttcacca 22200aggggctcag tccgctgatg cgccacacct acatctacaa caactacgcc tacggctgga 22260ttcccgagac cgcgctctgg agcagccgtc tgggcgacta ccgcgtcacg gacttctacc 22320cgatctcgct gggcatgctc aagaagttcg agttcatgtt ctcgctgctg gcggaccccg 22380gcggcgcctg ccccgcgtac gagcccaagc tcaacaccga gttcctgaac cgcggctcct 22440tctcgggacg gtacgtgaac cccttccacc gcttcgcggc gctgcccgag cgcgagtaca 22500tatccttcct gctgctgagc tcggtgccca tcttcaacat cctcttctgg tttaagggcg 22560agaccttcga cactgccaag cacagcctgc tcggcgccgt gtacaccacg cccgagcggc 22620acatcgagct cgcgcggtac ctgcggcgca cgggcgacta caagccgctg ttcagccgcc 22680tgggcaacga cgacacctac tcgaagccct tctctgggtt cacgcgcatc agcaacccca 22740cgcccatcgg gcggctgccg ccctcggact tcgagacgct ggccaacctg agcaccattc 22800tctactacac gcgctacgac ccggtgctct gtttcctggt cttctacgtg ccggggctct 22860ccgcgaccac gaagatcacg cccggcgtgg agttcctcat ggagaagctc tcgctcgcgc 22920ccgagaacgt ggtgctgctg tagcctcaaa cataaaatat aggcgccttt gatcgcactg 22980cttcagttca gacagagcta agatggcttc ctacatcagc ggcgctagcg ccagcgcgaa 23040caccgcccag ggcggcgatt ctcagtaccc acagtactat tatcacacac gcacctccca 23100aggcgacatc cgcgacgaaa gcgaaggttg cttccacacc acggacgacg agcacttgga 23160tctgtccgac gactacctcg gcgatggcgc accacactgc ggacacagcc acaaccacag 23220tcgcagagat ggagatcggc accgccagcg cgcaccgcgg ctctatgagg acccggtgcc 23280cgcgaacatc atggtgccca cgctcagtct agagcagctg ctggaggaaa cctcggtcgc 23340ggggggcctt ctcggcggca ggacagagag ggacgtggaa cagctcctgg aggagttctc 23400cgcactctgt cccggggacc agatcaccgc gctgcgctgc atggcggcct ccttttaccg 23460cgacgcgctg ttcgcgccgt acgcctgcat gcacctcatc gccagtcgga tgcgcgtgca 23520ctacgcgcgc gaggtcgtgc acgtggccga ggacctcgcg gacgcgatgt ctgcgaacag 23580cggcgtctgc ttccggcggt accgaaagcg cgtgctagag gacatgctcg cggaggagat 23640gggcgtgtac aattacctcg cgcgcgccaa cgcggacatc tgcgaggaca acctgctatc 23700ggccgtggag acgctgctgc ggcgcttccg tcggatgggc tgctaccgct ctctgtgcat 23760gctcaagatc ctcgcgctgc agcacgagga cctggccggc ttcatccgcc gcagcataag 23820aaaaacctgc aacttggcac acgcgcgcac gcacacggtt tacgtgtagt taccctgtaa 23880agacgggctt gctcccgaac aagcgctcga agaagagcgt gcacatagcc ttattgtcca 23940gcaagttgac tatctctgta cacagcctct tgaagtacac ctcgtacatg atccgctcgt 24000ttttatccag tctgaaggtc ttgtcgacca cgcgctcgta ggacttcacg ttcgcgatcc 24060ggcgccgcca ggggccctcc tcgcacacgt acgcgaagaa gtagcgctcg ccgatctcga 24120tggcctccgc gttcgccgcg ttgtaccgcg tcactagcgc cacgttgggg ttgtcggggg 24180acttgaagtt cttgtggtgc gttcggctca gcaggaacca gtccagcggc atgctgcgcg 24240cctcgaactc gaaggtgagc tcgtcctcca gcgagcgcag gatctccacg cccacgttcc 24300cggagccctc ctccgccagc gcgcggcaga gcatgtcctt gtacttgcgg atcatgagct 24360tgtggaaggg cgccacgtcg cggcgcgtct cgctggtgcc cttgctcacg cgctcgctgc 24420cgccgccgtc gctcaccgca aacttgatcg tggtgtactt cttcttggac tgcatgatca 24480ggttgcagta caccgcttcg aactccacct tgaagttcgc gaagagcacg tgctcgttga 24540tcacgcgctc cagacagcgc cccacgcgcc gcgagaacgc gatgtcggag gcgcccacct 24600ccaggaacac ggagtcggtg tcgccgtaca cgctgcggaa gcccacgcgc tccgtgcgct 24660cgccggccac cgccgcgtcg atctctagct ccgcggcgcg gcccgcgaag gcctcgtcgc 24720gcagcagcgg gttgtccggc gccgccgcca gcgacagccg cgtgccgcac accgacgcgc 24780cgtctagcgt gcgctccagg tacgcgatca tggtgcgccc gatggccgtg cagctcttgg 24840ccgaggcgta cgagaagagc gcgctgttgc ggaagcccat gagcccgtac acggagttgg 24900ccgtgatctt gtacgtgtac tgcatcgagt tgtagatctc gcggtccacc gcggtctccg 24960cggccttcat cagcttcttg tacttggcgc gcgcgtccag gaaggagcgc agcagcatcg 25020ggatgatgcc cttggcctcg cggtcgaaga tggccacctc ggcgacgagc tccggcgagc 25080gcggctcgca gggcaccgcg atgtaccgcg gcgccgggaa catccgccgg acgtccacgg 25140ccgcgacctc cgcgtcgagc cggttgtccg agacgaccac gccgacaagc gtctccggcg 25200acaggttcgc gtagatgcac acgttcgggt acaggctgtt gtagtcgaag atgagcacgt 25260gcttgttgtg catcttctgc ttgggcgcca tcacgcggcc gccctcgtag aagaacttgg 25320acttcgtgtc cgcgcgcacc atcaccgtgc ggttctccag cagcagcttc atcagcgggc 25380ccttgatgca ggtgctcgcg cggtactcga agaccacgct ctgcggcagc aggtacgtgg 25440acgcggcggc cgcgatcttg gtctccacgc cgtagtgcga ccagaggtag aggcagaggc 25500aggcgtcgtg caggcagtac cgcgccatgt ccagacacac gtccagcgag tagttcgcgt 25560acatgtccgc gaggctgacg tcgtccttgc cgaaggccag cgtgacgcgg tcgccgggcg 25620cgcgcgccgc ggggtccgcg aggtccacgg tgaagccgtc ctcgtcgacg cgtttgtgca 25680gcacgcggca cacgcgctcg tccacggtca cgtagttgcc ggtgctgagc acgcgagcga 25740acacggccgc gttcccgtcg gcgtcggtgc tgcggtcgcc gcgaaagcgg accgcgtccg 25800gccgcgcgtc ctccacgacc gcggtgcagt ggaaggcgtt cttggatatg gcgtccagct 25860tgtaggagtc cagcttctcg gtgcgctgga tgaaggcgta caggtcgaag tagatggtcc 25920cgttgttgtt gttgatgtgg aaggtggtgc tcgagacgcc gccgacgccc ttgtggctgg 25980acttcgtgcg ctcgtacacg cagaagttga ctgtctcggt cccgtccggc agccggaagc 26040ggatgtgctc gcccgtgagc agcgacagcc gcgagtccag gtaccgcagg tcgaagttgt 26100ggccgttgaa ggtgaccacg aagtccagcg gcatctcgag caggcgcttg gccacgcgca 26160gcagcgtcac ctcgggacac agcgtgacct ccgcgtcgaa cttcacgtcc gccgggtcca 26220ggcagaccgg gatctcgcgc cgtgccgcct cctcgaggtc cgcgtcggag agcatatcgg 26280agttcgtaag cgtgaatcgc cgctccgcgc cgtccttgtc caccacgcag aagctgatgt 26340gcgagacggc gttcttgaag acggaaggaa actttttctc gaagtggcac tctatgtcga 26400ggaagagccc cgagcgcgtc acgttgaagc gcgggatctt ctccgcgaag cacgcgccgg 26460ggtcgtcgca gtggaagcag ttgctcccca ggtcgcgcag cagcgcgggg tccacgcggt 26520agcacccgtc agggtcgatg tcgtgcgcca cgaagaacca ggacacgttc agaaagtcgg 26580acatgaagac ctctggcggc gcgagcttgc gctcgctggc caccagacac agctctatct 26640ccgagcgctg gcgctccgga atcttcgccg agcgcgctac gatctcgtcg atgctgacca 26700cggacatggg cccgagcgcg cgcgtccacg ccagcggctg ggcgatgtcg gccaccgcgt 26760ccgcgcgcac cacgtagtaa aagtgctgca cgaagcgcag gtacacgacg gcgttgtcgg 26820cgcggcgcgc cttgaggaag aggaaccggc tgtcattgcc gcggttctcg aaccagttca 26880aacatttcag ctccatttca aagagcataa taacatttca tttaaatgga gcctcgcttc 26940tggggccgcg ccatgtgggc ggtgatcttc atcgtgctgc ggcgcttcga ggagcaccgc 27000gacctcgagc gctgcaagcg gcagctgtac gtgatctgct ccacgctgcc ctgcatcgcg 27060tgccgacgac acgccaccgc cgccatcgag aaaaacaacg tcctctccag cgaggacccc 27120aactacgtgc tcttcttctt catcaagctc ttcaacaacc tcgccttcga cgacagatac 27180aagatcgacc ccgcgaaggt gcgcccgctc gtctagagca tgccctcgta cgcgcgcgag 27240ttgtccgagt acacggtcac cgcgatgccc tcgcgcggcg tcacgtgcac gtgcatggag 27300tccgtgatca cgaagcccac gcccgtgacc ggctcctcgc ccgcgtcgtc cgtgaagagc 27360agcccgtggt tgaagaggta gaactcgttc tctgcgagcg aaagccgccc gcggtcgcag 27420aggtagtaga agatgtcgta gtcgcgcacg gccagcgtga acgtggactc gctcaccacg 27480atgtacttgt ccagctcctc cagcacggac gcggccgcgc ggcgcgcggt ggcgcggcac 27540tgcgtcgggc actcgcacgt gtctgcagag tacaggatgc gcgttcgccc cgaggcggtc 27600tcgagaaaaa cgttaatcgc ctccatcgcc cagaagcgac tcgaggatcg cgagcaccgt 27660gcgcagcacg agcccgatgg tgcagaaggg aacctctccc gactccgagc actcgcggat 27720ctccgtctcc acgcggtcgt gcacttttat ggagggagcc gtcgttccag tggcctccat 27780cgcgacggac accaccttgg ccacgaactc gcggatcttg ctcatgcgcc ggagcacggt 27840cacgcggaag aagacggccg cgagcaggta ctcggccacg cagctcacgg cgatgagcgc 27900ctgcgcgtgc ctggtgttga gcacgtgcgc gtcgggcacg aagtccgaga tcttcaggtg 27960cgagagccgc acgagcgcgt tggtgtcctt gacgccgtcg caggaaatgt tcgcgaagat 28020gagcttctcg tagtcgagcg cctcgaccac gcgctgcgcg tccatgtgcc gctcgcccga 28080gagcgcgcgg ctcaaagggc cccagcacac cgagcccgcg aagcaggggt ccaccacgcc 28140gtgcatcgcc agcagcgtca cgtcggccac cgccgcgagg atggccgcgt cgtcgagctc 28200gttcgcgggc gtcgcgcccg tcagggacgc gctgcgcagc gcggacccgc ccggcgccgc 28260gtgccgcgcg cagaactcgc acccgcagcc cggcggcagc ggcggcttcg agagcagact 28320tatgagcccg cccacgtgcc cgtgctcggt gatcagaagc gccaggatgc tgtcggtgct 28380gccctctatg gcggctgtgg ccgcgctaga aggctctccc gtgacctgcc atccgcagac 28440aaccttgagc atcttgcgct tgagctcgtt gggcgcctcc gaggccagca tcgtgcgcgg 28500gaacgtcgcg ttgaggcgga agtcctgcag cagcttttcg agcgtcgcgg tcttggcgtg 28560ccgccctttg cgccactcct cccccaggtg ccacatgagc tcctcggccg tgtccagcgt 28620cggcgagacg cgggccttgg gcacgcgcgc cgcgctgcgc atcagcggct ccgaggcgcg 28680gaagctgccg cgcgcgtgca ggcgcatgga cgcagacgag gaccgcatcg agggtctctg 28740gtggaagctc gtcatcgtga agcgccgcgt gagcggacct acctcgtcgc gcgactcgtc 28800gaagtccggg tcagggtccg tcgtgtcggt ctcggcgctg tgcgtgctgg gcgcgctgct 28860gcgggcgctg cgcgtgctgc gcgtgctgcg ggtgctctgc gtgctgaagc tgcgcgagca 28920ggagtccgcc gtgtccgctt cctcgtagtg gaagtccacg tgctcctccg gcagccggcg 28980cgagcgcgac ttggagatgc cgaccatctt gtccgcgccg accgggcgca cgcagagcgc 29040catcgcgccc tcgcgcaccg cctgcgcgaa ctcgcggctg gcaaccatgc tggggttgag 29100gaacttgatg atgttgaagt atggccactc gcagacgagc cgcgcgcagg tcgcgacgtc 29160gtcgacgctt ctgagggccc tgttcagcgg ggggatgttg ctgccgtcgg ccagtgcgac 29220gaggtcctcg ggggagatct cgagcttggg aatcatctcg ttcacgagcg cggggtcgat 29280ggcgtggcag acggtctcta cctcggtgcg cgagagcttg agctgcgcgc aggccgccca 29340cggcgcgcac gtgagcgcga acaccgcgga gactcggccc ttgccgacca tcgccacaac 29400ctcgggctcg tagaccaggc cggccttgag cagagcctcg agcacctcta tgtcgtcggc 29460ggcgagcagc gcgcgcctgt ggaagcacac cgcgggcatc atcttcttgc agatgccgcg 29520ggtgctcttg agggccgtga taaggtcggg gagcctgacg tgctgcggct ggaagaacat 29580gacgttggcg gggctcgcgg ccaccgcctc gtcgtacacc gacatcggca ggtcgccgtg 29640gagcccgcac ggcagcaggc tcagcagctg cgcgcgcgag agcttctcgg cgcagaagtt 29700cttcttggcc agggcggccg ccgcgtcgcg ggccttcttg gggtataaca acatggcggg 29760ctttaaacac gaaacaaaaa tccgggttgt aacatttcaa ttttgcatgt tctgggcctc 29820ctcgcagagt ttctccaggc cgccggccac gatggcgtcg acgaagaggt cggcctcggt 29880gaagcggtgg ttgccgcgca ccgccaccag cgcgttctcg gtgaccacca ccgacagctg 29940ctgcgcagcc gtgcgcaggt cgaagtgtcg gcacgcgagc ccgtggccca gagtctggtc 30000cagcgccgcg agcacctcct ccaggctctc ctcgcggtgg ttgtagaacc acatcagcac

30060gaagtaggcc acgtaggtgt agaggtagtg cgcgaccgcg cgggcgcgca ccagcggctg 30120gttgcagcgc gcgaaggcca ttccgctggc gatgaggtcg tcgtccagcg tggcgtagtc 30180gccccagttg agcgcgctga actgcacgct gtagaccgcg cgcacgaacc cggactcgag 30240gatgtcgatc tgcgacggcg cgccccaggt caccagccgg tacacgatgc cgcgctgcat 30300cagccgcatg aggtcgccgc cgacctcgcg caggcggtgc gtcagcgagg cgaaggccag 30360cttccgctgc gtgtactgca ggcccacggc cacgcacccg tccgactcca ccagcacgag 30420cttgtggtcc gtgcagccgt cgctgcgcag gccgccctcg cgcgccatca tgtcggtgac 30480gaacatgtac ttgcgcgcgc gcggcccgac caggatgcgc ttcttgcctt ccatgcgcag 30540caccacgtcc tctagcagcc gcgtgctgtc cacgcgctcc acctgcgggt ggatggtggg 30600ctggtagttg tacagcagcc gcggggacca gttgtaggcg aacgcgaaga ggtgcgtgtc 30660caggagagag atggggaaga cgccgccttc ggtggcgcgc cggaggatgg cgagcttggt 30720ctccgcgggc agcaggctcc cggtcaccgc gccgaagaac atggggtggt tgcggaactt 30780gagcgcgtac gcgctcagca cctcctctcg cgagaatatc gagtccgcgg ggttggagct 30840cgcgggcagg agcacgtcct ccacgctcag cacctcgtcg atgaagggca gcaccatgtc 30900cacgttggag gcggtgaacc actccatgtc cacggcgttg cgctccacga tcacccggat 30960cgtctcctcg gatatgttct cggcgaaggc gctctgcagc tcgatcaggt tctcggtggc 31020gtcgatgctg agcccgaggc gcatgccctg ttgcagcacg tcgttgatgg ggttcacgta 31080catggccacc gccatgcacc cggccggctg cacgcgccag aggttggagt acgcggacac 31140gtccgtgatg cgcagcgtct ccagcacgtt gtacatcgcc gcgcgcattt ccagcgtgtc 31200cacgtacacc tccgcgtgct cgaagatgat gtcgagctcg aacgcggaca gcggcagact 31260cacgtagatc tggcaggcgt caaagagttc ctgcgcgtac tcagagaagc acgcgtacgc 31320gatcacgccc gcgcggttga cgatgtagtc cgccttgaac atcttcgtga ggtaggcgta 31380cagcgaccgg caggccacgt gcggccttag ctcgtcgagc acggcgtcca gcacctcgcg 31440gtgctgcagc acggaggggt gcaggaactg cgtgaagtcc accgcggtct catccatgaa 31500gtcggggatg gtctcgacca ccaagcggtg gttccgcacc gcgcggaagc cggtgttcat 31560cggcgcgatg ctgtgctggt cgtggacctc cagcaggatc tcgtagccga tctctcggca 31620gctcagcgcc gcgcgcgcct ccgtcacgct cgcgttccgg aagaactgcc gcaggtgctc 31680gtccgtgcgg cagagcgtga cggattgggg gatgcgggaa aggtcgcaaa gagggctgtg 31740gacggagatg cgccgcagcg cgtggtctac gaactcaaaa ccgcgcctcg acatcgtgaa 31800gtcgcggagg gcgtacatgt tgtaggaaca gaggcggaag aggcagtcta tgtctagcat 31860gttggaaacg cagtacgcgt gtctgtggag gtgcgggagc atggcgggca cgacctcggt 31920cgtgttctgg agcatggtgc atacgaggtc gggcgctgtg aggtcggggg tgacgatgag 31980gatgcaggag ctggagaact tgctgagctc ggaggccagc cggtgaaggt tgtagtcgtg 32040gcgctggctg aagttgctgt ttatcgtgcc cgtgaagccc atgagcgccg ccagcgtcag 32100gtcctcgcgc tcgatggccc agatgtctag gccggaggct atcatgcagc gcaccagcgc 32160ggcggcgcgg tcgctcgtgg ggtagctcat ggtgctgtcg gtcgtgcgaa tcagcatggg 32220ataatgcttc atttttacgg tcgggggttg cggactgtgg ggcgcacagg gcctgcgggc 32280ggctcgtgcc ggtccgcggc gttcgccgaa cgcaggaacg ggcccatgcg cgcccaggcc 32340atccacagcc ccgccgtcag cgccagcagc cagacgaata ccacgatcat cttttatgta 32400gctggaactc gcgctcactc cccgccgcac ggcgacgggg agagcccaga gccgagctcc 32460atgcgcgtgc tctgcacggt gagcgactcc acgagcttgg acacgttcat gcgcgtgttg 32520tcgggcacca ggtgcgcgag gcgcgcgtac acgtcatcgt gcatgcgctt gctgcagcgg 32580tccaggctcg cggagagcgc ggagactagc gcggtgtgct tcgcgtacac gaagtcgccg 32640agacacacgg tctcgagccc gagcacgtcg gcgctctccg cgtccttgag cgccacgaat 32700atcttctgct cctcgcgcgt catcgagcgc atgaggtagt cgtgcagccg cgagcgcgag 32760atgagcccct gagagatctg cgggctgcgc atgaagcgcc ggcgcatcgc gcacagcagc 32820tcctcgtcga cgacgtacat gctgtccttg atggagctct tctcgtcgat cacgagcaga 32880ccgtcgttgg cgaccacgtt gatgaaatcg tccacgtgcc gcgcgtctat gtcgtagcgc 32940gtgccgccgc actcgatgtg cgagggcggc gacttgagcc ggttcgcgag cgccttcacg 33000tcggagacgt cgatgtacag cgaggactcg cgcgggacgc agccgaggat gcgcgtctcg 33060agcggcgtga ggatgagcac gcgctcggcg ccgtcgacga gcttgccgtc gtcgggggaa 33120aagaagttgt tctccacgat gctcgagacg aggctggcga gcacgccgtc gcggtaggcg 33180ccgagcgcaa actcctgcac aaagggcgcg tgcagcaagt ccacgggaat gcgcatctcc 33240acgcggcgcg cgaccgactt cttcttctgc aggtgccgcc ggtccaccat ctcgtccacg 33300atgtccgata tgcgggagca gaggtacgcc ttgaggacgt tggcgtttac cttgttgaag 33360atgagccgtt cgtcctccat ttaagctgct cagacgagct ttaaatagtg gaaacacagc 33420agcacgccga tcgccgccgc tatcaggccg attagaaaaa cggtggtcca ggggacgccc 33480ttgggcctat cgcacgccgg cttttcggtc attacggtgc gcacgatgtt taggaactcc 33540tcgaagtcct cgtccgagtt ggagaggaag gagccgaaga cgccggtgta cagtttgtcc 33600atttactact agatattaaa cggcgcttcc aactcctcgt cctcgaagcc cgcgccaggc 33660tcgacgacgc ccaggccgcg cacgtcctgc tcctcgttga acgtggtctg agtctcgctc 33720atgcgcacac acgtctgctc gccctcgaga ccgagcacgg tcagcgagca ctcgcgcggc 33780atggtgatct tcttaaccgc gaaggtgact ttgccctcgc cgcccgagcg gtagaacacc 33840accggcgcca ggatgagcgt cgccatctgc gcgtcgcggg ttgcgaggtt ttctatctct 33900cgcgtcagcg gacatatctg cggctgggtg tcgtcgtcgc tggtgaactc caggagagcg 33960ccggtcaggc ggttgagata cagacatccg gacttaaagg tgttgtcgat ggccgtttcg 34020gtgttgaagt tgcgcagcga cggcggaacg cgagcgccgg ttttgatgtt gtcgtatatg 34080ttctccagca gctggtagag cagcggactg gcgctcacgg gcttgaggcg accgaagtac 34140ccgctgtcgt tctgccgctg catgtcggtc ttcttgctcg ggtagatctt aaactcgccc 34200ttcacgacga tgagcggcga gacgagcttg gatgctagac tttcgacgag acacacgttg 34260atgttggagc agctcgggta ctgcgacgga gttagagtca cggcctcgat gaccttggtt 34320tggctcgacg agagcgactt agcgaagttg atcgcgtcgg tgcacgacat cgcctggttc 34380tcgccgaacc gcctggcgga cgctgcatcc tcctgctgag gagcgcggtt agacgcgacg 34440gtggttttgg atacagcgcg tttcattatt gcggcgattt taaagtacgt gtatactttc 34500agttttgtcg ccgagcgttc agcgcctgca tgcagaggaa gtacaggatg atggtgcacg 34560ggatcgtggt cagcagcgat acgaagtcca tcactgtgag gacgcgcagc gccccgcgcg 34620agcggatgcc cagcgagggc gcgccgcggc gcgcgatggt ggccccgttc gtcaccacta 34680ccagcagcat taggatggtc gcgcccacgg cgacgcccag gtcccgcgac tccatttata 34740gtacagtata gagcgaccgc gtcacgaact ctcggctggc caacacgcgt ccgtcgggcg 34800ggtgtccgcc ggccttcccg cggaactccg ggacctcgaa gctggacttc gtcacgcggt 34860acgtgtactt gccgcgccag accaggtttt ccttctggaa gacgccgtcc atggtcacgc 34920ccgccatgaa ggcgtccttg acgatgacca gcaccgcgtc tagcttgcgc ccgttgatgt 34980gcgtgacgaa gtccgtgccg ctgcggctcg cgcagcggat gtccacgccc gagggcaggt 35040ccaccacgaa cacgaagcgc ttcggcgcgt agagcaccag gtccgaggac ggcgacgccg 35100aaggcgccga ggggaactgc cggtggtcaa aagggtgcac cacgcccacg atggacgtga 35160cgcggtcgtc cgggaactgc gtcgcggcgc cgccgccgcg gtgccgcgtg accgtgcttc 35220tgcccacgtc gtcgcagacc acgtgcagct ccgacacgat cggcagcagc gtggccagca 35280tgcggtcggt ctctgtgcgc gtcgcgcagc ggtacgcgat cccgcagtgc gcgtcctgcg 35340tgcgcccgaa gaagagcacc agcacgctcg cgtcctggtc gaagggacac acggccatca 35400cgcccaccgg cggcggcccg tggcctgcgt acgcggagga gaactcctgc acctcgacca 35460cggcgtcctc gcgcgcctcg ccgggcacca tcgccgccgc cggccgcagc gcccgcacgg 35520tctgcttaac cgcacgcgcg gcggaggccg cgctcggcgc gactacgcgc acggccgcgt 35580gcgcgcccgg cggcggcgcg gttccggcca tccagcccac cggcgagaag aacacgtcgc 35640agacgtgcac gcccgcggcc tgcagcgcgc gcgcgagcgc gcgcacggcc tcccactcct 35700cgcgaaaggc gctcgcgacc gcgagcgcct tcagcaccgt gtccacggag ttgacgggct 35760tctggaagag gttctcgttg ttgtagatga actcggggag ctccacgggc actgtgaaca 35820gcctaatctc gtgcgcgccg ctgggcgtga gccgcgtcgc gggcttgcgc acgccggcgc 35880cgatctgctt gaagaagtgg ttcatggcgc cgccggcttc tcgggctccg gcgggagcag 35940actatttatt cgggaggtta tcctttccga aagcacctgc acggacttcc gcgtccagcg 36000ctccatcttc atgtactcct tcatgccgtc gctgagcacc tcgacggcct ccagcttggg 36060cgctgtcggg tcgaagagga tgctcttgag cagcgtcatc ttcttgtccg cgaggaagcg 36120gaagtaagtg tagatgcagc gcagcgcgcg gaagttctcc gggtgcttga tggtgcacag 36180gatcatgaag atgcaggtga acatgccgca ctcggactcc atgagctggt tgacctcgag 36240gttgatgcag ccgcgccgcg ccttgaagtt gtccacgaag aagcgcatga gcacgtccac 36300gtcgcagttg cggttgtcca ggtccgcggt ctcggcgttc acgttgaagc cgtccgagaa 36360ggagtagaag tagaagtact tgcaggggtg gaactccgag gggctgttgc cgccggagtc 36420gtagaaggac acgagccgcg agacggtgtc gaagatgcag cacttccagt ggaacatgta 36480gcagaagccg aacatcacgt agcgccgccc ggcgcgctcg atcttgtcct tgagcgtgag 36540gctgaccatg ttgcagcgga agcggtccgc cttttcgtgg atggccgcgc cgttgaggaa 36600gttcaggttg aactggccca ggtacgcgac ctcggtgccg aacgcgaagg gcgccaccag 36660actctggatg ctcacgttgc tcatccaggc gctgcggtcg ggctttatgg cgatgggcac 36720taccttggtg ttcacgcccg tgctgacgcc cgcgcgcgcg aggtcgtcca cgttcagcgg 36780catctgcgag aagtccacgg cctcggacac cttctcgcgc aacgagggct tgaagaagaa 36840gcccagcggg accttccact ccagcgcgat cgcctcgcgg aagccgtagc gacccttgag 36900gctggccagc aacgcggtct tctgcgcgac ctcgtccttc tcggtgtccg gcggcgcggc 36960gtcgatgagc ccgcgcttcg cgaagtccag cagcgccgcc agcgggatgc acgagacgcg 37020accggccgtc gcggattcgt cgaagcgccg caccacgtac ccgttgcagt tggtcttgaa 37080gttggacacg tccaggtgcg cgctgagccc caccaccgag tagatgtggc acagaaggtt 37140ggtgaacccc agctccggga ttttgctcac cactaaatcc gtgtacttgt ccatttatca 37200tggagaatca tctgccggac atgctgatgt ttcccaactg cgtttctgtg tttccctttg 37260agtactcgct ggaggacgtg ttccgcctcc ccgaggagcg acggcgcgcg ttcgccatgg 37320ccgtgttccc gctctccaag caccgctgga ggggcgcgcg gctccagcgc gacgagcgaa 37380gcgtgtggct cagcgtcgag gaggaccgcg ggcgcgcgct ggacgagcgg aactgctcct 37440ggctctcgga cgtggccgcg cgcatggtcg acgacgaggg ccgcgcggtc acgcccgagg 37500cgtacgcctt catgcgcgcc gcgcccggcg cgcgcgtcgc cgagctcgcc gcggacgcgg 37560gcgtgctagc gggccttgtc gccggcggca acgcgctgcg cgtcttctcc tcggagtcca 37620cgcaggcgcg cgagggctgg aaggcgcgca gcgtgggcgt gctcggcaac gcggcgccgc 37680tggcgcccgt gccgctggca tcgctgcgtc cggaagtgca gcgcgagctc ttcgccgcct 37740ggatcggccg ccgccccgtg gtgctcacgg gcggcacggg cgtggggaag acctcgcagg 37800ttcccaagct gctgatgtgg ttcaactacc tcttcggcgg cttcgagcgc ctggacgccg 37860tccgcgagtt cgcggagcgc ccgctcgtgc tctcgctgcc gcgcgtcacg ctggtgcgcg 37920cgcacaccgc gacctacctc gcctcgctgg gcttcggctc ggccgacggc tccccggtct 37980cgccgcggta cggcgccatc ccggacgccg agcggaacac ggccccgcgc gcctacgggc 38040tcgtggtggc cactcaccgg ctcacactga ctgccatccg ccgctacgac acggtcgtag 38100tggacgagat ccacgagcac gaccagatgg gcgacatcgt ggtcgcggtc gcgcggaaac 38160tgggctcgaa catgcgatcg ctggtgctta tgacggccac gctcgaggac gaccgcgcgc 38220gcctggagga gttcctggac cggcccgcct ttgtgcacat agagggcgac acgctcttcc 38280ccatccgcga ggtctacgtg aagaacacgc aacagccgcc gctctcgcgc aagtacgcgg 38340aggcggagct gcagaacgtg gcgcaggcgc tgggcacctt cgtccccgag cagggaaagt 38400gcggcatcct cttcgtagcc acggtggcgc agtgcgcgct cttcgcggag accatcgagg 38460ccaagcaccc cgggctgctg gtgcgcgtgg tgcacggaaa ggtgccctcc gtggccgcgg 38520tgctcgagga ggtatacgcc gcggaccggc ccgcggtgct ggtttccacg ccgtacctgg 38580agtccagcgt gaccgtgcgc accgccacgc acgtctacga cactgggcgc gtgtacgtgc 38640ccgagccctt cggcggccgc gagaccttcg tctccaagtc catgtacacg cagcgcaagg 38700gccgcgtggg ccgcgtggcg cccggcacct acgtgcgctt cttcgacacg cggctcgcgc 38760tgccgctgaa gcgcatcgac tccgagttcc tgcacccgta cgtgctttac gcgcgcatct 38820tcgggctaac gctgcccgac gacctgctcg tgcagcccag cgacctcgcg ctgctgcgcc 38880gcaccgagga gtacgtcgac ggcttcggca tcagcctctc gcgctggacg cagctgctgg 38940accggcacta catgcacatg gtcgagtacg cgaaggtgta cgtgcgcggc gggcgcctcg 39000ccgccgcgct ggacgccttc gagcgcaccg gcgtgatgac gcacgaggcc accgaggcca 39060tccgcgccgt ggacatgctc gcggccgtcc taaacgtgcg caagtccaag gaccgctacc 39120gcgcggagtg caaggtgctc ttcgggccct tcgcgggcaa aaagttcgtg gtcgccgggc 39180ggcgtccgcc cggctcgcac gtgctcatgg tcacagaccg cgtcttcatc gaggccgagc 39240ccccattctg aggaccacct tcttggagac gcccgagaag tcgtcggcga cgccgcggcg 39300cgccaccaca aggcagtacg aggtcacgtg cgggcagcgc gcgatgcagc ggaaggcttc 39360ctcctgcgac agcgagaagg cgaacacgta gaaggtgtgc ggggacttca gcggcgtgtg 39420gtccatcgag tagatgacac cgagcttctt catgcgccac ataagcgcgt tgatgtggtc 39480ggcgcgcagc gcgcggccct tgagcacgcc gcagacgaag ctcgagcagg ccacgacgtc 39540gtagcgcgtg ttcctgccga agaccaggtg cggcgcgccg gcggcgcgcc gcgcggccgc 39600gcgattctcc acgatgtcct ctatggagcg ctcgctcgca aagaagtcca ggaacatgta 39660ctggtaggcc acggccgggc gcgacttgct gaacttcatg aaggcgtccg agtccatgat 39720ggcgtccatg tcctcagcgg cgagccggtg ctgcagccgg atgccctcga aggtgtggaa 39780gagccgcgcg tccgcgtgca tggacagcgc gagagtgacg aagttgagaa ggtccgcgtc 39840gccaaagcgc acgagcacgt taccgggcgt gcgcgtcttg cgcatgagcc gcgcgggcgc 39900gccgtcgttg tggctgcggc ggcgcatttt gtcgccgggg gactcgggcg gcaggtcgat 39960catgaccagc cggtgccgct gcgcgtcctc ggcgttgaag atcgaggacg tgaagcccgg 40020gtacagcacc acgcagtcgc gctccgagat ggcgtgcagc acgtcgcgct tgagcccggc 40080caccagccgc tccgcgttct cgacgaagta gttctcgtag tccaggatgt cgtgcgccat 40140ccaggggaag ttcaggtacg cgttcatggc gtagtcctcg gcgtcgaagc agatgcgcgt 40200gtctggcgtc gccgcgatcg gaaggtcctt gatgccgcgg agcagcccgt cgtagtcgga 40260ctcgtccacg aaggagagca ccacaaagag gtcctcgccc acggtttcgt agtcgaagag 40320gtggtaaagc tctcttagcg ccagcacggc gagcgcgttg tccagcgagg cgtgcacgcg 40380cgccaggatg ctgtagaagg gcgtggccat catcacggcc ttgccgccct cgcaggcaac 40440ggcgcgcggg aaaatgacct ccggcgtgcg cggcagccgc ccgaacgtcg cgttcagcag 40500cgcgaccgtg gccgcgtcgc tctggcgcag gaacactacc accgaggggc ccgagatgct 40560gagcatgcgc tcgcgcatgc gcgctggcag gtccggcgtg gtcacgaggt ccgcgaagcg 40620gccgccgttg tagaggtcgc cgccgccgag gaaggtgagc acgtcgaagc agtgcagcac 40680ctcgttgcgg aagtagtact cgttctcgag ctccttggcg tacgcgcgta tgtccacgtt 40740ctcgaagttt gttcgcagac cgccgccgtc gaagaaccag gacgcgagct cgcggacggc 40800gtccgcgggc ctgttgcggc ggctcttgca ccagaagctc atgtagttgc gcgaggtgga 40860ggcgttcgcc aggaagaagc ggtggtcgaa ggagatgagc acatgctcga gcaggtgcgc 40920gagccccagg accgcgccca cgtcgcgccc aaaaccgaag tttgatatcc ccaggtagac 40980gtcccgtttc atagacggcc tcaggaacac cctgacgccg ttttccaaca ctatcattct 41040ccggtattta cttacccaaa agtagtatgg ggagaagtgt ttgaacgtcc cctcgccttt 41100ttaaatcaaa agtagacttc tcgcgcccgt gcgccaccgt cacgcgcgcg cggcgcgagt 41160ccataccggc gatcaccgcg ctgctctgcg gtgcgtccgg ccgcgggaag agcacggtct 41220cggagatccc gtccagctgc gcgtcggtgc gctgccgcca cgcgtgcgcg tccgcgagct 41280cgcgcacggc cagctgcatc ttgttcgtcg gcaggaacgt gaacacgtac gccgccgcca 41340ggaaggctgc gaagagcacg aactcaaccg cccatgacat ttagggagct gattttgttc 41400cacgcggcga cgcacgtcgt gacgggcgac cccgaggcgc cgcggcgcgc ggcctcgctg 41460tgccgcggct tcggcgtgga cttccgcgcg attcacgcgg agttcgcgcg gcggtacccg 41520cgcaccgcgg ccgccgtgga gcgcgcgcag ccgctgcccg aagtcgatgc cgcctttccg 41580ccggacgcgc gccggcaagt cgtgcggctg cgcctcgagg ctgcggcgct ggtcgtcaag 41640gagtcgcgtg cgctatcggc ctccatgcgc ggcgtggcgg tggtcgacgg ctgctgcgtg 41700cgcgtgtgcc gcgccaacga cgagctgcta gagttcctcg cgcggcgcta cgaccccgcg 41760gtctaccgct acgcggaggt gccctcgccg agcgtgcgcc cgggctcgaa agtcttcgcg 41820tgcgcgggcc gcagcgtcac ctttgcggcc gcgcaccgga gccgcatcac ggccaaccgc 41880ccgctgcgcg tggtcgtgac cgaggcctgt gtggacggcg tgctcgcgcg cggcgccgcg 41940gaggtcttcg accgcggctc cggcgtgctg ccccgcgcgc tgcgcgagat cttctaccgc 42000ctcgacgagg acggctgtcc cacgggccag acgccaggct tcgcggacag tatggcgtcg 42060cgcagctgat ctatgtccac ctttttctcg tcgatctgcg ccacgaccac gaaactgcga 42120atgtccacag cggccatggt cttggccacc gggtcgtact tgaggagcag cacgtactcg 42180ttgccgaagt gctcggtgac ctcggtgatg agccggtaca cgcccatgcc gagcacgttc 42240accgcaccgt ccttggcgaa gagcgagagg atgttcacgc acttcagctc catctcgccc 42300tcgaggcgcg cgagcatgcg ccgggtgacc tcgcatactg aacaaagagg cttacctagt 42360aagataagcg ttagcttagc cgcggtcggt gacgcgtcgg aggccattta tggggatcaa 42420aaacttaaag gcgttgctgc tcagccacgg cgcgctgacc ccgcacgagc cgggcggcga 42480cgagcgcttc cctgccgtgt tcgtggacgg cttcagcgtc atgatgacca tggcgtactc 42540gtgcgcggac gaagacgagt tccgcgcggc cgtcgaggag cgcgtgcagc actggatgag 42600cgtgtccgag agcgggcgga tcgtggtctt cctcgaccgc ggcgagattc cgatcaagca 42660gccgctgcgc gaccagcgcc gcaaagccac gcgcgaccgc gccgcgcgcc accgcgagtt 42720catcgccgcc gcggaggcag acgcggcggc agaggccgtt ggcgcccgcg aggacaaaca 42780ggaggacgag cacgcggagt tcgccgagga gatccgcgct gagaagcagc taaagctgca 42840gcgcatccgc ttccagctca gcatcgccaa ccacgaggtc gttaagtcgc tgatagagtc 42900cacgctcgcg cgcgctggcg atgccgtgga gatcgtcttc tgcgacggcg tcgacgcgga 42960gatggtcatg tgcgcgcgcg gacgcgccga ggccgagcgt cgcgggcgct ggccgctgct 43020cgtgaccacg gaccaggacg cgcttttgtt cacgtccacc gatcgcgacg agaagatagt 43080gagcaccgtc tccgcctgct acgcgttcag gcccaccgag acgaccgagt acctgtgcaa 43140acttgcggcg ctggccaacg gctgcgactt cttccccggg ctcggcggca tatgcgtgag 43200tgtggagtcg ctgcgccgcg ccacgctttt cccggaattc tccgtgcgca acgccgccgt 43260gagtctgtgc acgcggccca tgcggctgtc cacgcaggac gcgctggagc cagaggccgc 43320cgccgaggtc gtggaattca tcaggcggta cgccgccggc gacgagcgca tctaccgcga 43380ggtgccgccc ggcgcgtgct gcggacgcgc gtttgtgcgc ggagcgctcg cggccgagtg 43440ggccgacgcg ctgccggcgg ccacgggtct gagcgtggtc gcggacatga tcgcgtgtct 43500gcccgcgcgg cgggaccccg cgcccgagga ggtagagcgg ctgctggcgc tggaggcgcg 43560cgcgcgaggc gcgcgcgtca cggatgcgat gctcgcgcag actgcgcagc tgctgggtta 43620cggcgcgagt gcgggcgccg acggcgcctc cgccttcgcg gtctcgggcg ccaagggcct 43680gatgtgtcgc ctgcgcggca cggccatgtt cttcaacgcg gagtacgtgg aaattgaaag 43740cgaacccaga ctgttaaagc tgcggtagca tggtgttccc gatcgtgtgc tcaacgtgcg 43800gccgcgacct gtcgcacgag cggtttctgc tcatcgtgcg acagcggccg ctaaaggttg 43860ttttgcggac ggtgcgcaac gtctgctgcc gtataaagtt gtctacacaa atagagccgc 43920accggaacct gacggtgctg cccatgctcg acataagctg atttttcttt tccgctcgta 43980tgcgcgagtt cggactcgcg gcgcgcatgg cccgcgccat cgaggacgtg tgtccgcgcg 44040gcgcggtgat attcgtatcc agcgccgcgt ccatgaccga ctgcctgaac ccgtcggtgt 44100tcaagcacgc ggcgatatac gcggggcgcg tggaccgcgc gccgctgccg ccgccctcgc 44160cggtcccggc ggaggccgtg acggagccct gtgcgataga cgccatagcg ccttacggcg 44220cgcgcgtggt cctgctctcg gagctgctgc ggagctgcgt ggccgttcag gcctaccgcc 44280tggcagtccc cggcgccctc gcgctcatga acctcgcggc cgacgcggcc ttcgagctcg 44340tgggcacgcc ctacggcttt aacagcgacc gaacgtactg cttcaagctc gttgccgact 44400gctttgctag cgtgggcgtg acaacgaaga ccaggcgcat catgggtcgc gacgtcgtgc 44460tcagccagga cttcctggag agcggcatgt ggaccaaggt gctggactcc gccgcggagc 44520cgccgtggct ggtctagaac agcggcggcg cgcgggtccc gagcacgggc cgcgccacct 44580gcagccgctg ctgcagcgcg cggcactgcg cctcggcgtc ggccgtctcg gcggggtcga 44640cgggcgtcgg agttgcggag gtggtcctga acggctgcgt gttcaccgag acgcggatgc 44700gctccttgca ggagcgctgc tcgatgcagt tggccagcat cttcatcacg tgcaggtact 44760ccagcaacac gaacttttcg agggtgatgc cgtcgaaggg cgacgacccc accacgccca 44820gcgggctgga caccgcgccg tcgagcacct cgccgcggga ctccttgcgc gcgcgctcga 44880gcaggtcctc tgtccgagcc accacactgc cgaagtcggc ggccgcgggg gcgggaacag 44940gcgcagcagc gctgtccgcg tccgccggca tctcctcgat cttgagaccg gccgcgaact 45000ccgaggccgc gtgcacgggc gaggcgccgc gccgcaccat gaagtcgcac agacgcgata 45060gcgcggagga gcgcaccggc atgtcgagca ggcgctcggc ctccatctcg gcgaccgagt

45120cggcgcacgc gtccggcgcg cccgcccgca cgagctcgtc gcagcacccc gcctccttca 45180tgagcgcggg catgagcttg tactgcgcca tgttcaccag cccgtacttg agctcgagca 45240ggtccgcgag ctcggaggcc atgggtcggt ttttggtgta gatgacgcgc tccacggcct 45300ccgccatgtc cacggcctgc atgagctcgc cgacgagcac gctggccacg agcgtggcca 45360gcgtgacgcg cacggtgggc acgcagaccg cgaagaagga ggtggagtgg gtgaagcgca 45420tgagcgcgcc gtgcagacgc gcgaggtccg cgctgttgcc cgcgtgcacg aagcgccggc 45480gcagccgcgc cagcgcctcc acaaggtcct ctcgcgtggt cacgcgcacg ttcgcgatgc 45540acaggtcgtg gatcgcgttg gcgatctgcg cgcggcgctg cggcgagctg ccgggcagca 45600gccgcgcctt ggcctcgacg tcgacggtgc tcgagagaca gccgcaggcg gcgccgcgga 45660cgacgaactt caacaacgac tcgaacacgc gcgcgcccgc gcggggcgct tgcttggacg 45720actccattta ctttaaataa tttacgagat caaaataaaa tgactctgcg catcaaactc 45780gagaagctca agcagatcgt aacttacttc tcggagttca gcgaggaggt ctcggtgaac 45840gtggacgtcg gcgatggcct catgtacata ttcgcggcgc tgggcgggtc cgtgaacatc 45900tggaccatcg tgccgctcag cgcgagcgtg gtatacgacg gcgatgtcag ccgcgtgttc 45960aacctgcccg tgctcaaggt gaaggcctgt ctgtgcagct tccaccccga ctcggtggtg 46020agcctggagc ccgacctcga ggacaacgtg gtgcggctct cgagccacca cgtggtcagc 46080gtggactgcg acaacgagcc cgtggcgcac cgcacgaaca ccgccatctg cctgggcatt 46140aaccagcgca agtcctacgt gttcaacttc cggcgctacg aggagaagtg ctgcggccgc 46200accatcgtca acctggacct gctgctgggg ttcatcaagt gcatccacca gtaccagtac 46260atcacggtct gcttccgcga caagaagatg gtgctgcaca cgcccgggaa ggtggacaac 46320ttcttccgcg agtactccat gaccgagtgg gcgcccgacc tcgagcgctt ctcgttcaag 46380atccccatct cctccgtgaa caaactccgc ggcttcaaga agcgcgtggt catgttcgag 46440tcgcgcgtgg tcatggacgc cgacgacaac atcatcggca tgctcttcac cgaccgcgtg 46500ggcatgtacc gcgtgaacgt gttcatgtcc tttcaggacc ggtctctttc atgcgactaa 46560atactcatgg gcgggtcggt gagcctgccc tcgcgggacc tgccgccgcc ggtgcgcacg 46620ccggagatga acatcgtgcc cgagcgcgac ctcgcggaca cgatggcgcg cctctccacc 46680gcagacccgc cgcagccgct gggcgtcggc gacgacgcgc gcatggccgt gctgaagacg 46740accttccccg agttcgcgat atcgcggccc gcgacgggca tgctcgccgc gcagcgaatc 46800aggtacgacg gcgacccgcg cgtctgctgc ggcgggttcg ggatctcgca ttactgggag 46860aagggggcgc gccgatcgaa cgtcgcgttc gagggcgcgg cgctgcgcac ctgcgacccc 46920acgcgcttcg acgcgggcgc gtgcgacgcg ctgctcttcc gcgagtgcgc cgccggcggc 46980gtcgacgcgg acttctgcgc gcactggatc aacgcggccg tgacgcggcg cacggaccga 47040cagtcgcgcg cgcggctgaa cgacatgttc gtgcgcgatt gccaaaacga cgccgcccgg 47100cctcactgcg tggcctggat ccgcgcgatg cgaagcgcgc gcgcgacggc ggacgacggt 47160ctaatagacg ccgtgctctc ggtgcagagt cccgagttca agggcaagca catgcgctgc 47220agctacccct cgccggccac tctcgccatg gccgcgaacg tggacgagcc gcgcgagtgc 47280tgggaccccg agtgcgtggc cgggaacgtg gacttcatgc taagcgataa ctacacgaac 47340ctgggcttgt gtcggctctc gcgctgctcc atcggcgtca cacacctgcg gattgacgcg 47400cgttcgcggc tgcgcatgcg gtgcgccggc gcgcttgccg ggctcacgaa ggcgcccgtg 47460aaccagactg tcgtcgtcgg cgacaacctc gcgcgcgcct tcgagccgcg cgtggaaacg 47520ctcagcgtgt tggcgctgtg cgtggtgtat ctgctaattg tctggctcta aatgggggcc 47580gccgccagca ttcagaccac cgtgaccacc gtcagcgagc gcatccgcaa cgagctcgag 47640cagagcgcga gcgctagcgc gaccgccgac tgcgacgtca ccatcgggag tctgattatc 47700cgcaagaacc taggatgcag cgtttccgtc cggaacatgt gctcggccaa cgccggcgcg 47760cagctggacg ccgtcatgaa ggccgtgagc agcaccttca acgacctctc gtcggaccag 47820aaggcctacg tgcccgggct gctcacggcc gcgctcaaca tccagaccac ggtgaacacc 47880gccgtcaagg acttcgagac gtacatgaag cagacctgca cggcggacgc ggtcgttcac 47940aacaaaatca agatccaaaa catcgtcatg gaagagtgcg cctctctgcc agggagtccg 48000gccacgcacc tggatttcgt gaacaccggc acggccgtgg gcaactgcgg cgtgaaggcc 48060gtgatggacg tgctcgcgaa ggccagcacc accgtgcgca acgaccagga ggccggcaag 48120ggctaccaga ccatcatcat cgcgatcgtg gtcgccatcc tggcggccat cttcgcctgg 48180tacgcgcggc acatgctatt catgtccacc tccgacaaaa tcaagctcga gctcgccaag 48240aagcccgtgg tgcactggac cacctacctg gacaccttct ttacggaatt tccgccgtcc 48300gtctagatac gcgcaacatt gaaacattat atccacctct caaacggcgg tatggtccga 48360cgcgtcctcc tcgagcgcgt ggacggcatc gtcgagcact cgcgcgcaga ccgacgctac 48420ttggaggcca ttcagcgaca cctcgagggg tctacgcccg ggctgcggca gatgtggcgc 48480ttcctctacg acctgctgct gacggtgttc gtcgtcatgt acatcgtctt ccgcctaatc 48540gtgcgcaacc ccggcatctg cgccatcctc gcgctcgcgg ccgcggtgta ctacctgttt 48600ttgtgtctct ttagcatgga ctgatggcga tcacagacag accatcgccc gcgcgcgcgt 48660gaccagctcc ggcgccgcga agacgtcctg caccgggaag tcgtcgatct cgaacacgga 48720gccgtccgcg gaccagatca cgcgcacgtt gtcgctcacc gagacctcgg tcagcgtcac 48780gcccagcaca accgcgtcgt tggtgctcac cagcaccagc gcgccgggct ccgcgcgccg 48840gtgcagcggc ggccccgaga ctgagcgccg ctgcacgcgg aacatgtccg cgaactgctt 48900cgagagcaag tccaggtggt tgcggatgat ccactcgaag aagtacgcgc aaccgccgcc 48960gccgcacagg aagcgcgaac ccgcgggcat cagcagccgc acaacgtcca tgtagcaggc 49020ctgcggcagg ctcgcgcggt acagccgcgt cttcggcgag agcaccacca ggctggaggt 49080gctcatctgg aagaccagct ggctaacgga gacggtgagc gtgcacgcgg gcacggaaac 49140cacgtccagg cagatgtcgt ccagaaagat gctccgctgg tagaggtggt acaggatggc 49200cacgatctga aaggccgtgg cgtcgctgat ggcgcagggg cggtcggcgc agcgcatctg 49260cgcgcaggac cagcccccga aggactcgaa gcagacggtg agcatgcccg tgctcggaca 49320gtgtggcgag cgccgacaca ccggaaagcc cacggccttg cggcagcgca ccatggtcga 49380gagctctatc cagcagcctg cctcctcctc gcccatgccc atggctaccg gcgtgaaggc 49440cgtgacgtcg tcgcagatgc gccgctccag aaaccccacg cccgaggagg ggtgcgcggc 49500cggcggcgag gtgatgcgcg ccgggacgcg gctcggagcg ggctcgggag gcgagctgcg 49560ctcgacccgg gcagtcgccg ccggccgcga tgccctgcgc gcgggcgcgc gctcgcgcaa 49620cttgtttgac ttgctggcct cgtcgctagc gtcatcgaag cggtcgttcc tgtcgccgcg 49680gacgtccgcc tcgtcgcccg tcggctgcgc ggcgggcgac gtgccgtccc gcgtacggcc 49740cgcgttcggc gcgaatgtca cgcgccggtg cacgtacggc tccgtagagc ccgtgggggc 49800gccgcgcccg cgcccgcggc ggaaggcctg ccgggacgcg ccgaagcggg cgaactcccc 49860cttcgcccgg cccctttttt cttccatgat atttatcaca aaaaaaactt ctctaaatga 49920ccaatctgct ttcgttggtc gacccggagg acctggcctt ctgcgccggg ttcccgtcct 49980tcgacgagac catgctcgtg atcgcggggg cgcgagtgcg cttcccacgc tcgctgctct 50040cgctcttcaa cgtggtgccg cgcaccatga cgcgctacga aaccgagctc gtgggcaccg 50100agatggtggt gggcgccgtg ttcaccaccg cgtacaacgt ccgccgcaac ctaggcctcg 50160gcgaggagcc cgtgaccatg cgcgacatcg agaagtactt cctggactcc gagaacgagg 50220tgctcacgct catcgtgcac aacaccgact tttccgccat gagcggcgtg cgccggcgcg 50280gcggccggcg catcgccaac cccgtcatct tccgcagcgg gtccacgccg ctgctcatcg 50340tgatggagtc gcgcaagaag accaacatct accgcgagcg caccgcggag caggccaacg 50400cctcctacag ggaggtcggc tcctcgctcg cgctggtcac tcggtacgcg ggtctgcagc 50460tggttgacgt gcacacgccc agctccgtgc taacggtctc cgccgtctac ggcttcaccg 50520aggacaaggg gctcaagaag ctgggctccg acaaggagct cgcggactac cagtccacgc 50580cgctcaccga ccccatccgg ctcagcgact tctccaatat attcgacggc gtcaagaaga 50640gcatccagct cacgaacgtg cccgtgccct ccaccggcgc cgaggccgcg ccgtaggctt 50700tcatgcgcga taaatcggat ggcggcgccg acgacgcccg cggtgcacct cacgccggtg 50760ttcgtggagc ctacgatcgc gcactcgctg ctgcgcgcag agtcctacct cgcgatcgcg 50820gtccttgagc tcgtgctcgc gctcgcgctc gcgctcgtct tcttccgcga cgagctaggc 50880tcgctattcc gctgcgcgcc gcgagcgcct tcgccgctgg acgcgtacct gcaggcgagc 50940ctcgtctgcg acggcgacgc gctgctgatc gagctgcccg agggccgggt gccggcgctc 51000gcgctggacg ggcggcccgt cgcgttcccg gggtgcgaga gccttttgta ccgcataaat 51060ggaccacgaa aagtacgtct tgtcgatgtt cttggaggaa gataactcct tcttctcgtt 51120cgtcgccgcg ctgtccgatg acgaggcgct cggcgccgtg cagtccgctg ccgccctcct 51180ggacttcctg ctctccgtgg tggtccgcgg caaggagaag ctcgccgccg cggggcacca 51240ctacgactcc atcgcggacg gacgcgcgcg cgccgcgttc gagttccgag acctgcgcga 51300gctggcgcag ctcttcgacc ggcggccctg cggcgtccag gaccgcgtgc gtgtgcgcga 51360cgggcccgcg cgcgccttcg tggacgcggc actggggctc atgcgcgagc gaggcttcga 51420cggcacgcag gccgcggagc gcgcgcgcta catcgcgccg aacgatctgc ccgcgctggg 51480ggcaatatcg gccacgctct cgccgggtct ataacgtaaa aaatattagt aaaattctga 51540aggtccgtgt gtttcgcggg cggccaacaa accagtcgct taaatggagg gggtggaaat 51600ggacaagccg ctcctctact tcgacgagat cgcgggcgcg cgcgactacg acgcggcctt 51660cgcggagaag cacgagccgc ccaagatccc cggccgcgga cagatgaagc tgctggtctg 51720cgagctcgtg tttctcaacc ggctgcacct gcacggcatg ctcgacggca gcgtcatcgt 51780gtacgtgggc tccgcgcccg gacggcacat ctgctgcctg cactcgcact tccaggagct 51840cggcgtctcg cttaagtggg tgctcattga cgggcgcaag cacgacccct gtctctcggg 51900gctgcggaac gtgaccacgg tgacgcgatt cgcggacgag gcctacctcc gcgagctgcg 51960cggcgagctg cggcgcgcca agatcgtgct catttcggac atccgctcca accgcgtgga 52020cacagagccc accaccgcgg acctgctgcg cgactacgcg ctccagaaca ccatggtgag 52080cgtgctcaag cccgtggcct ccagcctgaa gtggcgctgc cccttcccgg actcctggga 52140gaaggacttc tacgtgccct gcggcaagga gatgctgcag ccgttcgcgc cgccgttctc 52200cgcggagatg cggctgctca ccgtgtactc ggagacgcgc ccgaagctgc gtctgatcac 52260gctcagcgac gcggtcaact atgaaaagag gatgttctac ctcaatagcg tggtccgcca 52320gcgcgtaatt ctgaactttg actatcccaa ccaggagtac gacttctttc acatgttctg 52380tctgctctcg tcggtggtgt gctcgtgcga atttaaatcg cccaaagaga aggtgctgag 52440cctgcagaac cgcttcttcc gcttcctgcg catcccgccc tccatcacgc tcgggctgcg 52500ccggcacgat gaaccgccac aacacgcggt acctggccaa gatcctctgc ctaaaggccg 52560cggtaagaag cgaccccttc gcggtggtaa gtagggacac cgtgcgcatg tacgacatcg 52620aggtcgagta cggcgacctc gtgacggtgg tcaccgtcac gcacaaactc gagaccagcc 52680gcaccgtctt ccaggtcttc aacgagacct ctgtcgcgta ctcgccgctg ccggacgact 52740acggcgagcc catcgtgctc accacgtaca tgcagcgcga gcacaccaag ttcccgctct 52800ccatgctcta catcgacgtg gtcgcctcgg acatgttccc cacgtttaag cgccccaccg 52860aggaggaggc cgcggtggtc gcggccatgc agcgcgtggg cgggcgccgc gagcccgtgc 52920tcaagctccc gcgcatgctg gacaccgagc tcgtgtgcaa gatactgcac ctgcccgagc 52980acccgctgcg cgtggtgcgc ttcctgcgcc gaaacatgtt cacgggcgtg gaggtcgccg 53040accgctcggt gtccgtggtc ctcgactgac gaagggcagc acggtcagcg aggccgccgc 53100caccaagcac agcggcagcc acgcgcgcgg gtccgccacg ggcacgaaga cgtgctggtt 53160caggtacttc gcctggaagc gctccgcggt ggagtccacc ttggacccgc aggcgttggt 53220gaggcgcacg accgcgtccg cgacgcgcac gtccccgagc gatatcacgc agtcagagac 53280gttgcacccg gcgatgtttt tcttcagcgc gcgcggcagc agcgcgtccg cgcgcttgca 53340gggcgcgtac cagcagtagt agggcaggcg cgtgtcgcgg ccggtgtcga ccacggcctg 53400gctgggcttg aggcacgcgc agcgctcgtc gtccgggtgc gcgtcgcaga aggcgtaaat 53460ctcctcgtcg ggcgcgtccg gcccgggcgc ggtcggcggc gccgcgcgac ggcggaagaa 53520catctctgaa aaaatacttc gaccagaaaa cgaccaccga tcttatttca aagataaaaa 53580tactattaat acgcactcgg agaatcatgt cggtggtggc gcgcgtgtcg tacagcctgt 53640actcgcagag cgagataagc gccacggacg tggtcatcag ccagttgaag aacgacgagg 53700acctgggcac ggtgaaggac ccgcgcctgg gcgcctcgga cgggtccata tgccgcacct 53760gcgggctcac ggagatggag tgtttcgggc actggggcaa ggtgcgcatc tacgagtcct 53820acatcgtgcg ccccgagtac atccccgagg tggtgcggct gctcaaccac ctctgcgtgc 53880gctgcgggct gctgcgctcg cgcgacccgt acacgacgga cttggccgcg ctcagcgtgc 53940acgagatgcg caagatgaag gaccggatga tgtccaagaa gaaggcctgc tggaacagca 54000agtgtctgca gccgtaccag aagatcgtct tctccaagaa gaagatctgc ttcgtgaaca 54060aggtggacga gatacccgtc cccaacgcgc tcatctacca gaagctgacc tccatccacc 54120gcaagttctg gccgctgctg gaggtgttcc aggaccccgc gaacctgttc tacaaggagt 54180acatgcccgt cccgccgctg ctcatccggc cggcgatcag cttctggata gacaacatcc 54240ccaaggagac caacgagctc acctacctgc tgggcatgat cgtgaagtac tgctccatga 54300acgccgagga gcaggtcatc cagcgcgccg tgatcgagta cgacaacatc aagatcatct 54360cctcgaactc gagcagcatc aacctctcct acatcatcgc gggcaagagc aacatgctgc 54420gcagcttcgt ggtcgcgcgg cgcaaggacc agaccgcgcg ctcggtcatc gggcccgact 54480ccgcgctctc ggtgtgcgag gtcggcatcc ccgactacat ccggaacacg ctcacgcaga 54540aggtgttcgt gaactacctc accagcaagc gcgtgcgcgc gctgttcgag gaccgcgcgg 54600tcaagttcta cttcaacaag cggctgcgcc agctcacgcg catcaaggag ggcaagttca 54660tcaaggacaa gatccacctg ctgcccggcg actgggtgga gatccccatg tccgagggca 54720cgaacgtgat attcggccgc cagccctcgc tgcaccgaca caacgtcata tcctcgaccg 54780cgcgcgcctc gcccggctac accatcaaga tcccgcccgg gatcgcgaac tcgcagaacg 54840cggacttcga cggcgacgag gagtgggccg tgctcgagca gaaccccaag tccgtgatcg 54900agcagagcgt gctcatgtac ccggtgacta tcttcaagca cgacgcgcac ggcgcgccgg 54960tgtacgggtc catccaggac gagatcgtgg ccgcgttctc gctgttccgg caccagaacc 55020tctcgctgga cgaggtgctg aacctgctcg ggcgctacgg gcgagacttc gcgccggagc 55080ctggccagaa gaccttctcg ggcgccgacg tcttccgatt catgataggc gcggacataa 55140acttcaaggg cgtgctcgag aacgggcgcg tggtggcgcc gaacgtcgac agcgacctcg 55200tggtggccat gcgcgcaacc tcgctagcgg ggctgatcgc ggactacgcc acgaacgtgg 55260agggcgtgcg cttcgtggac atggcctcct acgtgtacaa gcggtacctg gccatctacg 55320gcttcggcgt gaccttccgc gacctgcgcc cggacccgag tttggttcgc cggctgcacg 55380cgctgaacac cgagaagata gagcagatca aggacgcgta ctcgcggtac ctgcaggacg 55440tcgcggacgg gaagctggtg ccgatggcgc ccgcggacga ggccgacgcg ctggactcgc 55500tgctggccaa cctgaccaac ctcaacgtgc gcgagatcaa cgagtacatg cgcgagacgc 55560tggagcgcaa ccccgataac agcctgctaa agatggcgcg cgccgggtac aaggtcaacc 55620ccacagagct catgtacctg ctgggcacct acgggcagca gcgcgtgaac ggcgccgtcg 55680ccgagaccaa gatatacggg cgcgtgctcc cgtacgcgtt ccccgactcc gcggacccgg 55740aggcgcgcgg ctacatcatc aactcgctca tgaacggtct ctccggctcg cagttctact 55800tcgcgatgct ggtggcgcgc tcgcagtcca cggacatcgt ctgcgagacc tcgcgcacgg 55860gcacgctcgc gcgcaaggtc atcaagaaga tggaggacac ggtcgtggac gggtacggac 55920agatcgtgag cggctcggta ctgctcaagt acgcggccaa ctacgcgaag atcccggggt 55980ccaccaccaa gcccgtggag ctgctcttcc cgcacgagag catgacctgg ttcctggaga 56040taagcgcgct ctggacgaag atccggcacg ggttcgtgcg catgcaccgg cagcgcctgg 56100ccaccaagat cctggcgccg ttcaacttcc tggtcttcgt gaaaccggcg ccctcggagg 56160cggaggcgct ctccgcgcgg gacctgtacc acatgatcca gcgcgtgatg aacgacgtgc 56220gcgagaagta cttcttctcg ctggcgaacg tggacttcat ggagtacgtc ttcctcacgc 56280acctgaaccc ctcgcgcgtg cgcatcacgc gcgcgaccgc cgagctcatc ttccgcaagc 56340tgtaccagaa gctgaacgcg ctgctcggcg gcggcacgcc cgtgggcatc atgtccgcgc 56400aggtgctctg cgagaagttc acgcagcagg cgctctcgag cttccacacc accgagaaga 56460gcggcgccgc gaaggtgaag ctgggcttca acgagttcag caacctcatc agcatgagcc 56520gcaaccacac cgagatagtg gcgctgaccg cgccgagcgc ggacaagctg atgccgctga 56580aggtaaactt cgagttcgtg tgtctgggcg agctcgtgcc cgagatcgag acccggccct 56640cgggacggcc ctccgtgcac cgcgtggaca tcacggtgca ccgcctgcgc atcaagcgcg 56700cgcacctgac cgaggtcctg gtggacacca tcatcgagcg cttcgtgtcc ttcaacgtgc 56760tcgtgaagga gtggggcagc gacatgaccg tggagggcga ccgcgtcacg tacacgctgc 56820tgctgcgctt cgtggagccg gagcagctca acttccacaa gttcatgctg gtgctgcccg 56880gcgccgcgaa caagggcaag gtgagcaggt tcaagatccc gatcaccgag accacggtct 56940acgacgactt cgacgccgcg cgcaaggcct accgcatgaa catcgagctc atgagtctga 57000aggagctggg gatattcgac ctcgaggacg tgaacgtggt ccccggcatg tggaacacct 57060tcgacatatt cggcatcgag gccgcgcgcg ggcacctctg cgagagcatg ctggacacct 57120acggcacggg cttcgactac ctgtttccct cctgcgacct gctcgcgagc ctgctctgct 57180ccgggtacga gcccgagtcc gtaaacaagt tcaagttctg gaacgcgagc gcgctgaaga 57240aggccacctt cggcgacggc cgcgcgctgc tgaacgcggc gctgcacaac cgcaccgacg 57300cggtcgcgga caacagcagc tgccacttct tcagcaagac gccctgcgtg ggcacgggct 57360actacaagta cttcgtgaac gtggagatgt tcatgcgcat ggagcgcgag atccaggcgc 57420gcgtggcggc gcgcaagatg gaggagatcg aggaggccgc cgaggaggag ttctaggcgc 57480gacggcgcct tactttgcga ccgtgtcacg acgacacgac acggttagga cggcgagtcg 57540cagacgaaca tttttatgag ctggtagcgg aagttggcgt tttccaggaa ggcgccgcgg 57600aggtcccgga tctcgtagta ggttttgagg aagtacacga agcgcgcggg ctgcgtcata 57660gtcgggttct ccgcaagccg cttgtgcatc acgtacccca tggcggcggc gccgctgcgg 57720ttgacgccgg ccacgcagtg cacgagcgtg ggcttctgct cggcctcgag gcgcgccagc 57780agcttcacga gcgcgggcat gatggaagcg atgttcgtcg tgtcgtcgtc tctcagcgga 57840atgtggtacg ccgttatccc cgcgggcgtc gagtacttgg acatggtcat gttaaccaga 57900cacttgaagt cgacgccgga gtccccccgc agcacggcgc gcgcgtcctc ggcgctgccc 57960aagtacacgt ggtccgtgag ccgcgtcatg cccgagggca gggccagcgg cggccccgcg 58020cgcgtgcacc gcagcaggag cctggcgtac cactcgctct tatcgcccat atttatttat 58080atgatacaaa tggcagacgt cacaacactg acggccaacg gtctgaccct ggagttcgcg 58140cgcgagcgcg ctctgcgcag tctgcgcgcc gcgcgcacct ccacgctggt gttcttcacg 58200ctcacgctcg cggcctcgct gttcgtgctc tggctgcagc taaccgagtt tcccgtcttc 58260gaggagctcg gcaagtacgc gcgcatcaag agcgcggtgc ggtcctggcg cccgctggtg 58320gaggctaaga cagagatcga gtccgacctc ggccggcaga agaccgccga ccggcccgag 58380ctcttcgagt tcaggtgcgt ggacttcggc aagttctacc tgccggtgag gtacagcccc 58440acgaccttcc tgccgcaagc cgtgcgccgc ggcgcgggcg atggctggat ggtgcacaag 58500gcggcggccg tggacctcgc cgcgcagcag ttctgcgagt ccgtgctgcg gcaccgcgcc 58560aacaacgtca tcacatgcgg gtcagagatg atgcggctgg tgggctacag cggctacttc 58620gaggacgacc actggtgcgc cgcgacgtcc ggcgtgctga cgtgaacgat cacacgatgg 58680ccgtgaccag cagcccggcg atgaaccaca gcagccgcga gttcggcagc agcagcacga 58740gcaccagcag gtacgccagg atgaagatgt cgaccacgtc cacgtcgaag agccccatga 58800aggagaagag cggcgtggtg aggaagtaga tggcgccggg ccagaagcgc gccagccacg 58860tggcgagcag cgaccacagg gagggcgcgc cgctgagccg cgtcttcacc tgtatgtagt 58920actcggggta gaccacctgc tcggcgccgg agagcaccac gcgcgccaga gagagccgct 58980tctccagcgt gaacacctcg gtgagcaggc cgctgcgcag ccctccctcc ttgatgatcg 59040cgtcgtagag cttcttcatg ccgccgacgc tgatgatgta ggcgtctagc gagacgtcgt 59100acccgccggg gtagaccatg agctcggggt cgccggtgcc ggggacgttg gtggccagcg 59160cgccggtcat gtaggtctcc ttgagctgcg tcatgtacca gccgttcgcc ttcatcgcct 59220cgatgagcgg cttcaccatc tcgggcttgc ggaaggtcat gtcgttgtcg accaccagga 59280tgaagtcatc gtcggagtac ttggtgggga cagtgccggc cgatatgctc tcccagaggt 59340tgaggtggtg cgctgcgcgg cgctgcatct ccttcggaca cgtggacttg cacatgtccg 59400tgaagaagtg cgggtagtct ttggagtcca cgtctttcca ttccaccgcc ttgagcacgt 59460ggtcgccctt ggggtgcggc gcgggaggag atggcttggg tgccggcgcg ggggccggtg 59520cgggggctgg ggcaggagag ggagcaggcg cgggttgagg cttgggcggg tcgtcggcga 59580ggcccaccag gtacggcagc gtggggaaca cctccttggt cccgcggcct tcggcaaccc 59640cgattatgta ggccgtgatt tcgggtggat ccatttagtt attaaaatta atcatataca 59700actcttttat ggcggctatg gattcggcta tccagtcctt gaccgagccc acgatgcccg 59760ccaggaacag gaagaaggcg aactccaggt ccacgcggtt cagagagtcg ctgaagtaca 59820cgaagacgtc gctgtccggg aagaagctgc gccggaacat gttgtacccg ttgaccttgt 59880gcgcgacgtg ctccgcgctc agcagcgtct cgtcgaaggg gtacgggtcg ctgaagcgga 59940acacgtacat ggccgggttt gcgtagtagt acttcatggt gtttgtgacg aagaggctcg 60000ccagcgagat gatgattttt ttcttctcga tctcgatctt gatgtggtcc tcgaagcgct 60060tcatgttgta ggcgttggtg tcgtgcacgc ggatgagcac gcgcgagtcc gacatgatgt 60120cctggaactc cgcgcgcgcg tcggggctct cggcgggcgt ctccgcgggc cgcgccacct

60180ccgcgcacac cgtcggccta gcgcgcggcg gcgtgcgcat gggccgcgcc cccacgcgct 60240gcgaagcgaa aaactccacg gcgcgagcct cgcccgcgtc cgcgtacgac tccaccaggt 60300agttgcggct gcgcgtggtg cggccgatgg tgttcagccg gtgcagctcc gcgaccagcc 60360ggcggtagtg cgcctccagc tcctcgggca tgatggaggt gtacacctcg gtgagcagca 60420tcacggtgtc gaagtcctcc ttgccgcaga cgcgcgtctt cacgaggaag tggtgcacag 60480ccgtcgcgat agagagccgc agcgtggact cggtgacctc gacgctggcg tccttggtct 60540tcttcgcgct ccgcgaggcc atgaacgaga cgaggaagtc cgcgctgctg ttgagcacga 60600tgaccagcgc gacgatgaag ttgaggttca gcgtcttcgc ggactggaac agctcggtgg 60660ccgacgcgtg cacgtcgagc aggttcgcgg agagccgcag gaagaacacg ccgcgcttga 60720tctcggccgc gaagcgacgt tcgtactcct gccggcgcgc gttgatcgcg atgaggaagt 60780tcaggatgag ccggttgatg ttgtacttca cggcccaggt ctgcgtcttc atgatggtgt 60840cgaaggacat cacgatgttg aagatgaagc gctggctgtg cgagaagtag ctgtagggct 60900cgctgaggaa gatggacttg ttggtcgcgg gcaccaccac gcccgcgcgc gcgccggacg 60960cgtcggtgtt caggtccggg atgttcatgc cgcagatgcg gcagtaggcc atgccgtcct 61020caaagtacac gaactcctcc acgaactcgt tgatcttggc gaagtagtcc acgtccacgc 61080gcatcgcgac cgcgagccgg atctggtgct cgcagggcgg cgactcgaag cgcaccccct 61140cgccccagcc cggcggctcg cgcacgacca gcgcggtgcg cgaggccggg cggaacttgg 61200cgtcgcgcgc gttgagcagc gccgggaaga ggtcgcagag gtgccggctc gagaggaaca 61260cgtacttgta cagcagccgg cgcgcgtccg cggccatggc gtccacgaag gcgcggcccc 61320actccgcgac cgcgggctgc tcctccgcaa agttgttcgg gtagaccttg tccgtggccg 61380cgaggaacac cttcttcacg tcgaggaagt cgcggatcac gatggggacg cgcgcgccgt 61440cgagctcgta catgaacacg tagcgcaggt tgagcttgcg ccgcgagacc gggatgccga 61500tgtgccgaca caggtacgcg aactcgaggt acttcttcga gaagcggatg cggtccaggt 61560tcttggagac gtactgcagc atgttgcgca tgttgaaggg gatctcgcgc acggcgggct 61620ccgcggcgtc gtcgaaggcg gtgcgcagat cgctggtgcg ctgtacgacc acggcttcgc 61680cggtggcgtc gtcgtgcacc agcacgttaa cgcgccgctg ccggatgacc atgtcgaagg 61740tgttgaagaa catctcgtac atgctgtgcc gagtgtcgtc cgcgatgcgc tcgcccaccg 61800agaggctcgc ggtggcgtcg tcacgcacct gcttctcgaa cttgtacccg atgtaggaga 61860atatcgagat cagcgtggcg tcgtcggcgt cggggttctg ctccatggtc gcgaagagca 61920ggcggatgtc gtcctccgtg atcgcgtcca cgttgtacag gttgaccacg aagatggact 61980tgttctcggc gatgaagtcc gtgtaggact tggtggccgt gttcgggtcg cgcatgtacg 62040cgcggatctt cggcacgatg ctcgcgagga tggactccct ggaatccatt taaggacggc 62100aagggcgcgc gagaccgtct caaaactgaa atcgtataaa ctcttaaaaa atcggtattg 62160aaagtacgca ccaccaaata aagcgtcgag gtcgggcatg tcttcgtggc gactcaaaat 62220gagcaagtgt tcaggttcca gcagcgtcca gactctcgag gatctgcgta atcgtcttcg 62280ctccgaggcc ttgggcaacg attgccaaga gccccgcgac gacctcttcc ccagcggcga 62340ggagtgtctg gacatcgacg ggccctgccc ttgcgatgag gcggagcagg agatcgacca 62400ggagcagttg cccgtgcccg aaaccgtgcc cgaaccgccg gccaagactc ctaagcgccg 62460accagtgaag aaggataagg cagataaggc agataaggac aagtcgacca gaggcgcaaa 62520gaaaccgtgc ccttcggacg acaaggatga cgagctcaag agcaacgacg tcgacaacaa 62580cgaagagtcc ggcgacacag acggcggcgc gagcgcccga agccccagcg acatcgacaa 62640cgtggacgaa atggacgact ccgacctcat ggtggcgttc tccaccatcc tcgcagactt 62700caaggacctt acccaacgag tgaaagctct ttcgtccgtg ctcacggacg tgcaggcggc 62760cggcatacgc aggagcttct cgacgctcgg caaggctctg acggaggcgg cccacatcgc 62820caacaccgga tctaagccag tcactgcgcc tcgcaagaag aaggccgccg cctgcaagaa 62880gtaggcgcac taaatagcga ggctcggtat gcgggcgctg cacctgtcag acggcaaact 62940tttttttgac aaggagctga cgcagccggt ccccgacgac aaccccgcgt acgctgtcct 63000tgcgaagatc cggatcccac cgcacctctc ggatgtggtc gtgtacgagc aggacctcga 63060gtctgcgcag cagggcctca tcttcgtcgg gcgcgacgcc aagggccgaa agcagtactt 63120ctacgggcgc ggacacgtgg agcggcgcac ggccgtccgc aacgccgtgt tcgtgcgcgt 63180gcaccgcgtc atgaacaaga taaacgcctt catcgacgac cacctcgcct ccggcagcga 63240ggccgaggcg cagatggccg ccttcctgct catggagacg agcttcttca tccgcgtcgg 63300caagacgcgc tacgagcgcg agagcggcac cgtgggcatg ctcacgctgc gcaacaagca 63360cctcgccgag gccgagggcg gtgaggagat ccgcgtcgcc ttcgtgggca aggaccgagt 63420cgcgcacgag tttgccgtgc gcgaggggca gcggctcttc gcggcgctgc gtcggctctg 63480ggacccgggc gcgcccgaca ggctgctgtt cgaccggctg agcgagcgcc gcgtgtacac 63540cttcatgcga cgcttcggca tccgcgtcaa ggacctgcgc acctacggcg tgaactacac 63600cttcctgtac aacttctggt ccaacgtgcg ctcgctggag ccgcgtccct ccgtgaagtc 63660gctcatctgc acctccgtgc ggcagaccgc cgagacggtg gggcacacgc cctcgatctc 63720gcgcagcgcc tacatggcca ccgcggtgct cgagctcgtc agggacggcg cgttcctgga 63780cagagtcgcc gccaccgaca cgctcgacga cttcgtggac atcgtcgtgg actatgtaaa 63840taactctgag caggtaaatg gatgaggcgc tgcgcgtggc ggcgcgcgtc gtggacgggc 63900tccggccgct ggacgtggcc gtgtgtctcg cgcagctgcg cggagccgcg cccgagcgcc 63960gcttcccggc gctcgacgag tgctccggcg aggccttcct ggactttgag ttcgccggcg 64020gggacgtggc gtcgcggtac ctctccgcgc acacgcgcga gctccgtgcg gcggagcggc 64080gcgagcacat ggccgcgatc gcgcgctgcg tcaccgaggc cgacctggcg ctcgcagacc 64140gcccccgggg caaggcgcgc gcggcgctgc gcgtgtgccg caaccgcgag aaagtcgcgc 64200gcttggcgag gctgctgcgc gacgccgaga gcagcggcgc ggacttcgcc ttcatacgcg 64260cggccgtggc gtagcaaaac gtaaaaacaa cacattccct aaatcgccat ggacgcgcca 64320agtctcgact gcatgctcgc cgcactcgcg gcgaaggcgg cctcggtgga ccgaggcgct 64380cccgaggacg aggtgcacca cgaagtggag ctcgtgctcg tggacccgcc gctgtccacc 64440ctggccgcca cgctgcgcct ggcctcggag acggagtcct tcatcctctt cacggtgacc 64500gcgctcgcca aggaggaggg caagctgcgc gcgcgcgtgc ccatgtcgcg cgtcgtcggc 64560ctggacgtga agaacgtgca gctggtcaac gccatcgaca gcatcgtctg ggagcgcaag 64620gcgctcgtgg aggagaccgc gctgcaggaa ggctgtctgc tgcgccactc caccgagcgg 64680cggcacctct tcgtggacta caagaagtac ctctcggcca tccgcgtgga gctggtaaac 64740cgcgtgcgcg tgcgctccaa agaagtcgtc gcggacttca agttcaagta ctttctgggg 64800tccggcgcgc aggccaagag ctcgctgctg cacgcactca accaccccaa ggtgcggccc 64860tcgcccacgc tggagttcga ggtcgtcccc gcgggcgagg ccgtggacga ggccgccgtg 64920ctcgcggagc tgcgcgccgt ggcgaaggcg ctcttcatgg cgcccaccga cgccgtcttc 64980ctggcgccgc cggccgagat gccggtgcgc acgctcatgc tgcagaagca ggagatcccc 65040gcgctagacc tcgacggcct tttcgcggtc tccaagacgg acggcgtctc cgcgagcgtg 65100tgcgtggacg aggacggcgt cttctgcgcg ttctcgcacc tcgcgtacac catccggtac 65160ccgctcgcgc gcgaagtgca gggccggtac cggctctggt gcgaggccgt gcggcccgtg 65220ggcgagcgcg tgtggtccat gttcgtgctg gtcgtggagg agcctgcggg cgatgaccgc 65280gtcgcggccg tggccggcgc cgtggaggcg ctgcgcggcg tgtgtgcacg cgtcgagttc 65340aaacctaagc gcgtggacgg gcccttctcg gcgacctccg agctggtgga gcacatcaag 65400agcgcgctgc agacggagcc agagggcgtg gtgctcttct acgcgcgcgg agagaagtcc 65460aagcgcgacc tcaaggtcaa gcgcgacaac acggtggacc agaccacgaa cgtgatgttc 65520cggtacatgt ccagcgagcc catcgtcttc ggcgagggct ccaccttcct ggagttcaag 65580cggtacagca acgaccgcgg gttccccaag gagtacggcg cggggcgcat cttcctgcgc 65640gaggacgtgg tctaccacaa caacatctac tgcatcgagt tcacgaagac gcacctggag 65700gtgggcctcc gcagcgtggt cgtgcccgtg aagttcatcg gcgagttctc gcaggagggg 65760tacctgctgc ggccgcgcct ggccaaaacg gagtgctact tccgcaaccc ctcattctac 65820gggaaccagc actcggtggt gctcgagcac actcgcgacc agctgctctc ggtgggggac 65880gtgttcgacg agagccgcat ggccgccgtc gggcagacgc tggccaacga cgccttccgc 65940ctgaacccgg acacgcccta cttcaccaac cgacgcacgc gcgggccgct gggcgtgctc 66000tccaactacg tgaagacgct catgatatcg ctgtactgct cgaagacctt cctgaacaac 66060gccgagcgac gcaaggtgct ggccgtggac ttcggcaacg gcgcggacct ggagaagtac 66120ttcttcggcg agatcgcgtc catggtggcc acggacccgg acgcgcgcgc gatcgagcgc 66180gccatggagc gctacaaccg cctcaacgcg gggctgaagt cgcgctacta caagtttaac 66240tacatccagg agaccatccg atccgagacc tacgtggaga gcatccgcca ggtcatgtac 66300ttcgggcgct tcaacatcgt ggactggcag atggccatcc actactcctt ccacccgcgg 66360cacttcgcca cggtgatgcg caacctgcgc gagctcaccg cgcccggctg caaggtgctc 66420atcaccacca tggacgggga cttcctgtcg acgctctccg agaagaccag cttcgtgatc 66480aaccgcaacc tgcaggagag cgaaaacttc atgtcgatcg agcgcgtggc cgatgaccag 66540gtcatggtct acgcgccctc gaccatggcg cagcccatga cggagtacat cgtgcgccgc 66600gcggacatcg tcaagctctt cgcggacaac ggcttcgacc tcgtggacca cgcgaacttc 66660gagaccgtga tccggcgcag ccgccgcttc gtcgagggcg tctcgcggct ggagacgcgg 66720ccctccacca agaacttctt cgagctcaac cgcaacgcgc tcacggagat ggacagcacc 66780gacgtggccg cgctgctaaa gatctacgtg ctgtacgtct tcagcaagcg gtaggcagaa 66840ccagggcgtc gattccgcgc ccgcgccggc gcggaaggcg ttgaacagct ccgccagcca 66900ggctgcggtc tcgcgcgcgt cgatcgggcc gccgtcgtcc ggcggcggct cgcgcgccgc 66960gcgcaacacc agcgtctccg cgggcggcag aggctccaga gcctcgaaga ccgcgcggct 67020cgggaacagc gcgcgcatca tgcgcgcgcg gtggccgaac accgccttga ccgcgcgcag 67080tgccgagcgg ttgtccagcc gcagcgctcg gtcaaaacga tgcacgcgcg cgggcgcgcc 67140gcggtggtcg cgctccacga gcacgtgccg ccacgccagc gccgcgccga cgcggtccag 67200gctgggcgcg agcgccacca ggcttttcag cgcatgtaaa tctccgcgca tggccgacgg 67260ctccatttac tactgcggag gaacgcacgt ggtcgcggcc gcgccgggcg ccgcgcttgt 67320ggtgctggac gcgcccggtg cggcggcggc ggccgcgccc gcggggcagc gcgtcttctt 67380cgccgagtac ggcctcgaga agcgggccgg cggcccgatc acggcgcggc tgcgccgctc 67440cgggttccgc ggcgccgcga acgcctgggc ctccgtggcg gacttcgagg ccggcggccg 67500tccctccgcg tggacgctgc gcgcggagga ggcttcgcgc gtgccgctgc cgacggacgc 67560ggcgctggtc ctggcctggg gcgcgcgcga ggagccgctg cgggcgtgcg tgctggcgcg 67620cgcggcagac gcggaggcgc cggtgggcgc cgcgctcaaa gaagccgcct tcgacgcgcg 67680ggcgccggcg gccgcgctgt tcgcggcgct gggcgcgccc gcgctcgcgc ccccgctgcg 67740ggcgcggcta gtggcgccgc cgggcgcgcc gccgcggacg cggctctgcg agaacccggc 67800catgctgcgc gcgttcgcgg tgggctggtt cggcgcgcag ctgggcgagg cctccgaaaa 67860tgaaaaggta tttgccgcct ttgataaggc gaggtcgtgt ttggacgacc gctgatggcg 67920acgcccgcga acgcgcccgc gctgctcgtc gcggcgctgc gacaccgccc gtaccgcgtg 67980gagtaccacc cggactggga gccggtcatc gagacgctgg tggacgagta cgacgcggtc 68040gcgccctggc tgctgcgcga cgcgacgagc cccgagcccg agcgcttctt cgcgcagctg 68100gcgaagccgc tggcggacaa gcgagtgtgc gtgtgcggca tcgacccgta cccgcgcggc 68160ggcaccggcg tgcccttcca gtccccggac ttcagcaaga agaccatccg cgcgatcgcg 68220agctcggtcg cgcgcacgac cggcacgcag ggctacgcga actacgacct ggacgcggtt 68280ccgggcgtgc tgccctggaa ctactacctc tcctgccgcg agggcgagac caagagccac 68340gcgatgtact gggagcgcat ctcgcggctg ctgctgcagc acgtggccaa gcacgtgagc 68400gtgctctact gcatggggcg cacggacttc cagaacgtgc gcgcgcgcct ggacgtgccg 68460gtgacgctgg tggtgggctt ccaccccgcg gcgcgcgacg ggcagttcgc gcgcgagcgg 68520gccttcgagg tcatcaacgc cttattggag ctcaacggga agtctcaagt ggactgggcg 68580cgaggatttt ctttttatag tgaaaattaa tccgtggtcc taaatggcgg cgcccatatg 68640cgataactct cacgtgttcc tcctcaagcg cctgggcgtg ccgtcttcct gccggcgctc 68700ggaggacccg cgcttcgtgg agatcctgac tcccttcgag ctctcaaact acatcgagcg 68760gcacccggga tgctgcctct tcgagacgct gcgcgacgag gaggactgct ccgtcgtgcg 68820cgtcttcgcg gacgtggaca tggacagcgt gctcgaggag gaggacttcg tcgcggcgct 68880ggaggacctc atcgtggagc tcgcggcctt cttcgaccgc ttcgcgagcg gctcctgcgg 68940caccgtgccc ggcgaggtca agcgcgccat gctcgcgaac ttctcggtca cgcgatccac 69000ggccgagcac aagaccagct tccacctgat cttcacggag acgtacacca cgctggacac 69060gctggtggcg gcgaagcgcc cgctgctgga cctgtgccgg cgctcggaca acgtgctgct 69120gcgcgcgctg gacacggccg tgtaccgccg cggcgcgacg ctgcgcgtgg tgggcacgcg 69180caagacgccg gagtcgagcg cggtccaccg catgcagtcg cccgacgacg acatcaagga 69240ctacctgttc acgttcgtgg agctctcgga cgcgagcgtg tacttcgagc tcgcggagcg 69300cgagcagcac acgctgagca ccgtctgctg ggagacctcc tacatcccct tcggcgacgc 69360gatgcggcgc gtgtgccagg cggtggtcaa cgacatcgtg aacctccgcg acatcaccga 69420ggacaacttc ctcgacacgc cgctggtcat cgactacgcg acgcgctgcg cgctgtgcaa 69480gaagcccaag cacaagcacg cgcaccacat caccatgggc aacggctgcc tgcgcctggt 69540caagggcggg aacgcgcaca gctgcaaggt caagatcatc cagctcgagg gcaaccggct 69600cttcacggcc gcgcagatca tcatcgcgtc cgaggtcgtg aagctcaccg agcgcaacga 69660ctacatcgtg tggctgaaca actcctggcg cttcagcgcg gaggagtcgc tcatcaccaa 69720gctcatcctg gacgtgcggc actcgctgcc cgcggactac gccaacgaca tgctgtgtcc 69780gcgcaagcgc aaggtcgtgg agaccaacat ccgcgacatg ctcgtggaca tctccgagac 69840ggacacgcag tacgacaagc tgcccttcac gaacggcgtg ctggacctgg ccacgggcga 69900gttcctcacc ggcgaccgcg cgaaggcctg cgtgtgcacg gtctccaccg ggtacgcctt 69960ctcgcgcgag gagttcgcgg ccgcggcgga ctcggaggcc atgcgccggc tggtcggcgt 70020catcgacgac atccagccgg acacgcccga gaacgccgat aaccgcgcgc tgtacgagcg 70080cgccatgtcc agcgcgctct gcggcgccac gaagacggtc atcgtcttct tctacggcga 70140caccatgacc ggcaagtcca cgagcaagcg tctgctcatg tccgcgctcg gcggactctt 70200catcgagacc gggcagaccg tgctcacgga cgtgctcgac aagggcccga accccttcgt 70260ggccaacatg cacctgcggc gcgcggtctt ctgcagcgag ctcccggact tcgcctgcaa 70320caacgcgcgc aagctgcgct ccgacaactt caagaagctg accgagccct gcatcgtggg 70380ccggccctgc ttctccaaca agatccacaa ccgcaaccac gccaccttca tcatcgacac 70440caactaccgc ccggtcttcg accgcgtgga caacgcgctc atgcgccgcg tggcgctggt 70500gcgcttccgc acgcacttct cctcgtcggc cactcgcgcg gccgccgcgc acaacgtcga 70560gtacagcgcg gtcaaggaga tggacgagag cctggacacc aagatccagc gcaactactt 70620ccgctacgcc ttcctgcgcc tgctcgtgca gtggttcggc aagtaccacg tcccgcaggt 70680ctcgctggcg cccacgcccg acgcggtccc cgacttcgcc ttccaccgcc gcgtggccga 70740gctggtggtg gccagcaacg acgcgcaccg ccgcgcgatg gagtcgctgt ccaagctggg 70800gtacgtgctc gtgggcggca acgtggccat gcccgcggac gccttccggc agcggctggc 70860cgcgcacttc aacgcgcgcg tgcacggcgg cgacatagac gccttcatgt tcaagcacaa 70920gaaggtcgtc aacgtaacgg aggagtacgt ggagtacgta ttcatcgaag atgtcgagaa 70980taaatagacg ggtatgaact cggacgtgat aaagctgttc gtcgggcacg acgagtccgt 71040gcccggcatc ctgccgcacc agctcgcgac cgtggacttc ctgatacgcc gcgttctaga 71100cgacaacgtc agcgtgcttc tcttccacat catgggctct gggaagaccg tcatcgcgct 71160gctgttcgcg atggtggcct cgcgcaccaa gaaggtgtac atcctggtgc ccaacgtgaa 71220cgtcatgaac atattcaact acagcatggt catggtcgct aacctgttca acgcgccctt 71280cgtggccgag aacatattcg tgtactcgac gactagtttt tattcgctaa actgcaacga 71340cggcgtcata aactacaacg gcctcggcaa gtacgagaac tcggtcttcg tggtcgacga 71400ggcgcacaac atcttcggga acaacaccgg cgagctcatg atggtgatca agaacaagac 71460gcgcgtgccc ttcctgctgc tctcggcctc gccgatcacg aacacgccgc tcacgctcag 71520cagcatcatc agcctcatgt ccgagaagga cgtggacgtc ggcgacatcg tggtgcaggg 71580caagaaggtg ttccagatcc tgctgaacga gcacggcgtg cgcgtgatcc gcgaggtgct 71640caaggggcgc atctcctact acgagatgcc ggacacggac atgcccgagg tgctctacca 71700cgggcgccgc ttcctggaca cgcgcgtggt ctactgccgc atgtcgcgcc ggcaggagga 71760cgactacctc accgtgcgcc ggctctgcaa caacgagatg ttcgagaaga acatgaacaa 71820cgtgtccatg gcggtgctgg gcccgctgaa cctggtgaac aacctggacg tgctcttcca 71880ggcgcaggac aaggacctgt acccgaacct gcgcatcagc aacggcgtgc tctacgggaa 71940cgagctcacc aagctggaca tcagctgcaa gttcaagttc ttcatctcga aggtgggcgc 72000catgcgcggg aagcacttca tctacttctc caactcgacc tacggcagcc tggtcatccg 72060caacgtgatg ctcagcaacg ggtactcgga gttcggcggc tcgcagagca acaatccgca 72120caccacgccc gacgggcgcg ccaagacctt cgcgatcgtg accagcaaga tgaaggcctc 72180gctggaggag ctgctcgagg tgtacaactc cgcggagaac aacgacggtg gcaagctcat 72240gttcctcttc tcctcgaaca tcatgtccga gtcctacacg ctcaaggagg tgcggcacat 72300ctggttcatg accatccccg acaccttctc gcagttcaac cagatcctgg gccgcgccgt 72360gcgcaagttc tcctacgcgg acgtggccgc gcccgtgaac gtgtacctca tggcggcggt 72420gtactcggac ttcgacgagg acatcgtctc gctggaggac tacagcgtgg aggacatcaa 72480cgcgctgccc ttcgacgtga agaagctctt ctacctcaag ttcaaggcca aggaaaccaa 72540ccgcgtgtac gccatcctgc aggagctctc ggacgcgtac tccgcgcgcc cgcacccgca 72600gctcgtggac gtggtgctgg gggagatcgt gcgccagttc ttcgcacggc actgccgcgt 72660gcccgccgag gacgccgcgc tcgtggccgc cgtcgaggcc gttctcggca cgcgcgaggc 72720agcggccgag tacatccgcg cgatagtgga cggacacttc ttcgtgacca acaagacctt 72780cgggaagtgc ctgctcttcc ggcacgagcg cgacatcgtg accgtgccct tcgagctcga 72840gcacgacccc ttcgcgtggg cgatcaactt ccgcaaggag gtcagtgtgg tgaatatata 72900acggcaaaca taaatagaaa gactgtcctt ttgcgcgatg tcgaccttcc ggcagacggt 72960gtacctggcg gtgacgctgc agccgcacga gctcacgctc gacttccgcg gcaacgtcgc 73020ggaggcggtc atgcgcgagt acctctacaa ggagaagggc gggctcatgg ccaccgacat 73080cgaggtctgc ctcggaaacg agatgccgct ggggcgcatc gtgaacaacg cggttgtggt 73140ctcggtgccc tgcaacgtga ccttcaagta ctaccgcgtc ggcgacaccg tgagcggcac 73200gctcaacgtc gaggacgaga ccaacgtctt cgtggactgc ggagacctca tctgccagct 73260cggcaagagc tcgggcggcg tgaccttcaa cgagtccaag tactgcctcg tgcgcaacgg 73320agtcgtctac gagcacggca gccgggtctc ggctgtgctg cgcgaggcgc gctccggacg 73380cgagtccgcg ttcgtgttct ccgcagtgct gctggacggc gtccccgccg aggagaagga 73440cgagaagaag gacgagggcg agaaggccgc ggaggaggag acgcccgcga gccccgccgc 73500caaaaactag cattattggg ccgcgcgaac cttcgataaa tgcgcacgta cacgtcgctg 73560ctctcgaagc tgctcaagag caaccggcgg ctcgggagca cgcgcgtctt ccgcgacccg 73620ctgcagcaca tcagcgcgac cgcctttgtg caccggcgca tcgaccggca ccggcgcgtc 73680tccatctgcg ccgtgctcac caccaccgac gggctcgtgg tcgcgtgccg gcgccggtac 73740tcctttttgt cctccgagct cgcggagacg cgctcgcccg cgcggcgcgt gctgctcgca 73800accaagcacg cggacgctct cgcgcgcctc ggcgccgcgc gcccgcgcga cgacgtcatg 73860tttccgggcg gcgccccgct gtccggggag tcgccgctgg cgtgcgtgct gcgcgaggtc 73920gaggaggaga ccgggctgcg cggcgaccag gtcagcgtgg acgagcggct gttcgtgcac 73980gccttcatcg acgacctggt ctcgggccgc gacttcgacg cgatcatctt cacgggcgca 74040gtcgcgcttt cgagcgcgga ggtggcgaag cagttccggc ccaacgacga ggtcaagggg 74100ctggtcttcc tgcaccccga ggacgcggag ggcgtgggcg tgatggcgcg gctggcggcg 74160ttcgcgcgct gcgcggcgcg cctgcgctgc tggggcgcgg ccgtcacgcg atagaggcgg 74220ggtccaccac gtacacgagg cgcccgccgc tcacgcgcac ggtgggcggg tcgcccagcg 74280cggtcaggaa gttcccgtcg tcgtcgaaga ggcgcccgcc gcgctcgagg aagcccttgc 74340gcaccgtgac cagcgccgtg gaggtggagt accacacgct ctgcccgtcc gcgagccgcg 74400ccgcgcgcgc gggcccgcgc gcgtccgccg ggcgcgccac cagcgcggac cagccggagt 74460cgtcctccag cggcgcgaag tccgtgaagg cctcgcgcac ccactccagc gagcagcgct 74520tgagcacgcg gaagagctgc gtgaactgcc gggacttgtc gcggatgagg gccagcaggt 74580cctcgtccac ggtggcggcg ccggagtcct ggcgcgcgac cacgaagtgc acgttcacgt 74640agcggcggtc gggcggcgtc atctcgtggc tgttcaggcg caccgcgcgg cccacgatct 74700ggcgcagcga ggcctcgttc caggtcatgt ccaggatgaa gatgtcgttg atggagagga 74760agctgaggcc ctcggagccg ctcagcgaga acacacagac cttgatcttc tcgccgtcgg 74820tgttgtcgca ggcgttgaag gcgtccacga gcttggcgcg cgtgtcgcgc gtgcgcgagg 74880agaactccac gctggagacg ccgaaggcgc ggaagtagag cagcagcatc tcgatgccgg 74940tcacgttgac gaagggctcg aagaccagac acttgcccgg cgaggccagg atgcgcaggc 75000agacctcggt gtacttgcag ctgcgctcgc gcagctccgc gagcagcgag acgtccgcgg 75060aggtcatgcg gtcgccgctg acgggcgcgc cgctgcggaa gagccgcatg gccgcctccg 75120agaagacgcg gtccttgacg gcgcgcgcga agtccaggaa gagcgcggcc acggcctcgt 75180cgtactcctg cttggagagc acggacttgt cgggcgcgtc ctcgaaggcg aaggtggccg

75240cgatgcgccg gtacacgcgg aagaccgcgg cgccggactt gcgctccatg gcggccgcgc 75300ggcggtaggc ctcggtctgc ttcgcggtca tgtccacgta catcatgcgc acgcgcttgc 75360gcgcgaaggc ggcggagccg tcgacgtcgt cgaagatgga ggcctcgttg gtgactaagt 75420acgagcacag gccgccgagc ttgtccacga ggtcctcggg gttcgcgagc gcgccgccgt 75480tgaagagcgg cgtctgcccg accacgccgg ggcgcagcag gttcacggcc atggagaact 75540ccttgacgct gttcaccacc ggcgtggccg tgaggcagag cagcttcccg cggcccatgg 75600ggatgttctt cgcgaggtag ttgtacaccg tgcgcgcggg ccgctggcgc ccgtcctcct 75660tggtcagcga catcgagatg aagttgtgga actcgtcgat gaccacgcag acgcggctgc 75720tcgacgaggc ggtcttcatc agcgtgaaga agcggtggtg gaagcgcggg tcgtcgtagt 75780tgatgaaggt gcacccgggc acggcctcgg gcgcgaagcg catcatcgtc gaggtccagg 75840gctgctccac gagcgccttc ttcacgagca cgaccaccgt ccagtccgtg aagacgtcgc 75900gcaggtgctt gagcacgtac accgcggtca cggtcttgcc cacgcccgtc tcgtggaaga 75960gcagcagcga gtgcatgctg tccaggccca ggaacacgcg cgccacgaag agctggtagt 76020ccttgaggcg cacggactcc tccacgccct gcatctcgga gggcatgtgc gcggtgcgcc 76080gcagcgcgta gtcgatgtag gccgcgtgcg cgctggtcat ggcgacggtc ggcgctcctt 76140ttacggggtc tgtcgtctat ctattgtcgg cgcgggtctg atttaggggc agtagttaca 76200aaaacgtttc cgctgctcgg cgcggcgttt ggaggagcgg ttgcggccgc ggcggcgcag 76260gcgcgcgcgg cgcgtcttcg tggtgcggtg gccgaaccag cgccggtgca tgaccgggtg 76320cgagaccgcg gccgcgcgat ccgcgctcat gcaggttgcg taggtgcggc acatgctgcg 76380cagcacgcgc cgcgtgcgcc gctccacggc gtcgagccgc ctcgcgacga tgggaaagag 76440ccggcgccag ccgcgcacgg cgaagagcgg gcgctcgcag accgggcgcg cgagcgcgtg 76500gtaggcgccc agcagccgcg ggtccagcga gcgcacgtag gtctccacga agccgttgcc 76560gaagacgatg gcctgtgcgc atagcgggtt cgtcatctcc ctcttggagg cgatggcgtc 76620gcccacgaag gcgcgcacgc cgcagtgccg cagcaccagg cgccgccgcg ggaagtgcag 76680gtgcgggccg agcgccgcgc gggcggcggg gatgtgcagc cgcggagaaa aacgcgcgcg 76740tccctccatg gcatctaagc gctccgtctg ttttcagtta tatcgccgcg ggcggctact 76800gcagcagcag cttgagcttg cgctggctct cgttctcgat gctcttggac tcggaggtca 76860tgctctcgta gagcagcgag tgcgtgacgt agagcgcctc gtacacgcgg ctggcgaagg 76920ccacgaagcg gtccacgaac tcgctctcta cggggtcctt gagcacgcgg aagggcacgg 76980ccagcgcgtc gcgccaggcg gcggccttgg tgcgctcgcg cacgtgcgtc acgaaggcgg 77040cgatggccgc gcgccgcggc tcgctggcga ccatgacggc gctgtccttg agccagctgt 77100cgctcacgca cttgaagagg cgcacggtgc cgaagaggct gcagtacacg cgcagcgcgt 77160gcaccacgtc ggtgccgaag agcgtgggca gcttgagcac cacgaagcgc tcctgcgtga 77220tctcgagcag cgggcgcatc accgcgaagg tgatcgcgtg gtagtcagcc acgtagaggt 77280tgttctcggt gaggtggttg ttggagcgga tggcgccgcg ctccttgcgg tagagccggt 77340tgcgcgcgct gaggtcgagc acgaccgcgt cggccctgcc gcgcgctctg gagcgcacgc 77400tggtgatgcc gtgcgcctcg agcaccttct cgacgtcgcg ctcgtcgatc atgaggtcgt 77460gcgtgtacag actcagcatc tccgtgggca tgcggttgat gtcgttcacg cgcgagcact 77520gcaggaagta gttggtcccg taggccaggc tgggcaggtg cccgacgccg agctgcaggt 77580ccagcgcggg cgtcgagtcg aaggtgggca gcgtcacgct gagcccctcg cggatgctgc 77640ggcgcacggc ctccaccgcg tccatggccg atttattgga cgcacagtct gttttcattt 77700cgcggctact gcgcagtcac cttctcggcc acgatccccg cgtcgtagct gagccggtac 77760acctcgttgc acaccacgac catctgccgc ggcacgtaca tgagcgggtt gtgcgcctcc 77820atgtgcgcgg tggtcacgcg caccgccagc ttgtccttgc ccctggagac gttggagttc 77880agcgcggtgg gcgagaagaa ggtgctgggc gtaaagttga actgcagcgt gcgcacgccg 77940ggcgtcttgc cgaggatctc gccgaagacg cgcgagactg cgctgttctc cgagtacagc 78000acctcgttgc cgaagcgcac gtccatgcgc gcgatgacgt cgatcttgtt cttgaagtcc 78060acgcccttga ggaaggggtc ggccacgaag aggtccttgg cgcgcgcctc cggcgagcgg 78120ttgtcgccgt tgtacacgtt gcgctggcag gtccacacgc ccacgggcac ggaggcgtcg 78180ccgatgttca cggagtggat ggcggtcgtg aagcggatgc gggaggtcgc gcggctgtag 78240gcccccgtga tggcggagaa cttcttggac atgttgtaca cgacggagtt cttcctggtg 78300gcgaacacta ggatgttcgt gtgcaggaac acgcgcatgc ccacgggcac gtcgtcgatg 78360cgcacgaaga cgtcggtgtc ctggatggac acgacgcccg acgggggcac ctcgactatc 78420tccgcggtct cggggaagcc ctcggggtag cagttcgaga cgatcaccat gtcctccagc 78480aggcgctcca cgaaggccat cacgaagtcg ccctcggact gctggaagcc ggggtacgat 78540atgaagcggt tgttggcgtc gctgagcacg ggcttcatgt acacggacag ggaggtgcac 78600gcgtgcacgt ccgtgatcac cgcggtggtg tggttgatct gctccacgcg ccgccgcggc 78660atctcgatga aggccgggcg cgggcacagg ttcttgacca tgtagccgat gaagctcagc 78720tccatggagt aggggaactc cttggcgagc ttggcggcgt cgaaggtctc gtcgtagacc 78780atgacgcagg cgacggggtt cagcgtgacc gtgaccgtga ccttgctgtc gctgagcttg 78840agcgtgctga aggtcttgtc cgcgtcaaag ggcgtcttga tgtaggcgtg cacgcaggcg 78900gcctccttga tgacgtcgtt gggcgagctc ccggtggaga ggtcgttgag ctcgcgcgag 78960aagcccgaga gctccatcac gcgctcgttg tccaggcagg agtcgaacag ctcctcgccg 79020gaggtctccc agatggtgtc cgcggcggag ttcacggcca cgtggcggat gagcttgtac 79080gcgatgtagg gcacgtagca catcttgccc acgcccttta tctcgggcag gtccacgctc 79140agcacgaagt tgttcatggc cgagatgtac ttgtcgcgga tctcgaaggt cacggtgacc 79200gcgtcgctgg tggtgtccac cacgccctgc gtggtgatgt actgcggcat gtacaccgtg 79260ggcgcgcggt ggtccgtggc gaacacgctg gcgcgccgca cggcgtcgtc gccgcccacc 79320aggctcacca cggagttatt catttattcc ctgggaaaac cagttaaata aggctcttca 79380gagccatgcg caccgtccgg ccgtcgggct ccaggtagca gcgcccgtag acgccctccg 79440tggcgcgcgt ctcgttgatg agcgcgcgca cgcggtcggg gtccgcgtac atctccagcg 79500gcagcagctc gatcttgggc tcctcgcgca gcgcgacgag gtgccggatg gagcccgcga 79560aggagtcgcg gcacaaccgc gagcagaact cgcccacggc gccgccgtcg agcgtctcca 79620cggccagcgc ggccgtgccc acgcgctgac ggcagaacca gcacgtgccg tccgcggcgc 79680gcagcgccag ccgctccgcg gacaccgtgt tgaagtactt cggcagcacg tactcgatgc 79740ggcacgccgc cggcggcgcg caggccgacg cccgcggcgc ggatatgtcc acccgcgaga 79800gcgcgatgcg cttcatgggc ggcggtggct gctatttatg tcgcccgcgg cttttcaaag 79860gtcgagcgag cacgccgcga agcgcgcggg cgagaacacg tactcgtggc cgaactccgg 79920gatctgcgcg gcgcgcttgc gcgcgcgcat gtgcgcgagg aagttctccc aggtgagctg 79980gttgctgttg ttcttcgcgt agttcttcac ggtctgcgga cgcaggttgc gcgtcacgcc 80040cgtgacctcg aagatcttgt ccaggaagaa ggagtagttg atggttttgg tgggcgtgat 80100ctcctggcag aagaagacca gctgcttgaa tatctcgatg acctcgttga tcttctcggt 80160gctgaggtcc agcttctcgt ttttgacctg gttgatgatc tcgaagacca gcttgtagtc 80220cttcttgttg atcatttcgc tgtccttgag gaagctggag acgtagttgg cgtccacgtc 80280ctcgggccgg atctggtgcc ggtccatcat cgcgcgcagg tcgcggatga cctcctccga 80340gcactgcttg gagagcagcc gccggagcac gttccgcagg tggatgagct tgttcgacac 80400gtggaagttg gacctcttct gcacgcggat gcccatggga aacacggtct cgcagaacag 80460gcagaactcg tagtccgcgt cggacacgag cccgttgcgg cggcagccgc cgcacatgcg 80520caggttcatg cttgctccag ccccagcacg cgcaggatct cgcggtccag cactttagtg 80580tccagcgtgc gggttctaca gaactggagg aagcccgcga gcgcgcgcgc gcgccctggc 80640tgcgagagca gcagcatgcg cgcgttctcg gggtcctcgt tgatgacgcg cgtgaggttc 80700agcgagcacc gcgtgcagcg ccgcggcggg tcgagctcga ccgagtacgc cgcgaaccag 80760acgttgtcgc ccatgtatta tttattaaca cagaacgtcg cacatgttgc gcgaggacat 80820gtacgggtcg tactcctgcc cgtagatgag gatggtgcag taccgcgaga tcatgagcat 80880ggcctcctcc atggtgagca ggtcgtcctc gaacatggcc ttgtgctgca tgtgctggct 80940ctgcttggcg gccatcgcgg ccgcgccgtc gccgcgcagc cactcgttca gacactggcc 81000gtcgccgccg gcgccgctct cctgcgcgaa ggtgttgcgc atggcgcgca tgagcctggc 81060ctccctggcc gagcggctga gcacggtcat ggggtcgtag agccaggggc ctgcgtccgt 81120gaacaggatg gtgcagtacc cgttggcgaa ggcgtccgcg ccgctgcagt tgtcgatgcc 81180gtcgccgacc ctgtagcaca ccgcggagac cagccggtac atgatgccgt tgagcatcat 81240gtcctgcgac acctcgatgg gaatgtcgct gatcacgggc cgcatgttcg tgaagcagtc 81300gcccgtgctg gccatgcccc cgcgccggtt caccaggaac accagcacgc cgttcgtgat 81360cacgggcgcg cggtcgcgct cgtagaggta gccgctcgcc gcgcacacgg cggacgccac 81420gtccgtgcgc gagaccgcgc ccatgttctg ggcgggcatg tacagcactc gcccggcctc 81480cgcggtgcac gagaagggct gctccccgcc cacgtggatg ggcgccgtcg atgtcgtgat 81540catcttgctg gagtccacca ccaggtaggg caccgtgtgc atggccatgt cgcccatgcc 81600cccgatcccc gttccgaacg acggccgcga cacgctcacg agcgtcggct tgaacgagac 81660tatggagaag atggaggcca agatctgctc ctcgtcggtc atgatggagg cgcacgaggg 81720gtggatgatc ttcattaggg cgttgtcgat ggactcgtcg ctctcgcagt agaagacgcc 81780catgcggagg ttcaggatgc accgccgcag gttggtgtgc agcaccgcgc gctggatctc 81840catggacacg gagtcgccga cgccgggcat cacgatgggc gtttcctcgg tgagcttgtt 81900caccagcagc tggtagttgt tgggtcgcac gcggctgttg tggtggagct gcgccaggag 81960cgagaggctg tccccgttga cgaacgcgga ctcgatggcc ggcagcttta cgccgaacag 82020cgccatggcg atggggtgca cgaagcccac ggagtcggac gacttgaacg agaagagcag 82080gtcgcccgag gagctcatgt ctttgaagtg cacggactgg aactgcgtgg cggagagcaa 82140gttctggtag ctggacatgc tctgcaggtc gtcgatctcc ttcatctgct tgtttacgcg 82200ggtcgagttc ctcccgtaga tgatcaccag cgggtgcgtc tggctcacgg atagccccga 82260gtccgtcatg gcggcgcgca cgctgttcag cagatcgaac agctccgacc ggtctctgtt 82320ctggatcccg acctttgcca tcacagacat gagctcctgg atggtcatgt tcttgtggtc 82380tcggcgcgtg gaccgcaggt agtcggcgat catctcgccc tccttacgga tcttcatctg 82440ccagtcgtgc atggaggtca tgcggtccac aggcatgagc acgctgtcgg aggacgactg 82500cgcggcgctg ccggactggc gcgagccagg gcgcgcggac gacgggcgcg cggagctgcc 82560gcggctggag gaggacctgg acctccgcga ggatcttcgc tgcgaagagc tgcgcacggg 82620ccgctgggcg cgcgccccgg cggaaaccat gtcctcgcgg tttatgctga ggagcgagct 82680gcagaccgcg cacgacagcg actgctttgg aatgtggatg tggtcgcact ccagggacat 82740gcccgcattg tcgtagcccg ggaccaagtc gaactttgca ttaaaaaaat ctgatgcgca 82800cgcgggcgat tccatttata ccggaagttt ttatgaggtg ccggtattat ccacgcgatc 82860tcgcagtgtg ctgggagact ctagcgtagc cacggacccc gtgagcagac gacgcaagtc 82920gttgatggcc gactgcgtga cggacttcgc cgtctcgatg tcgcgcgtaa ggctgagcga 82980ctcggcgttg aggtcgcgca cgctgtccgc gatgtcggcc agctcctttt tgatgaaatc 83040cttatcatta tcggcgttga tgactttgtc cggcactcta gactctagaa ccggtgacgc 83100ggcgggcgcc ttgatcgtcg ggcagctgga cgccgggtac tggggcggcg gcaatgattg 83160ctgtaggaag atgggcttag cggcaggcgc cggttttgtc ggcaagggcg gcgccggcgg 83220gcactgtcgt gtagacggcg ggcacgccgg cgcggggcac gccgccggtg gcggacatgt 83280cggcgcagtt gcgggtggac acgcgggcgc gggcgccggc gcgggacacg tcgcggcagg 83340agccgggcac gcgggagccg gagctggagc cgggcacggc gcggcaggag ccgggcacgt 83400cgcggcaggc gcagggcacg cgggagccgg agccgggcac gccggcgcag gaggacatgc 83460cggcgcagcg gcaggacaga ccaccgctgt cgcggagcac gcggcaaccg gcgtggagca 83520cgcggcgggc attacgttca gaggcgtcga ctggaccttg gcaccgcggg gcagtagcga 83580tggcgctagg ggcgactgtc cggtggttgg acgctgcatg caggcagtcg cagatttcag 83640acgggactgg taatacctgc ccgcttcctt caccgtgtac ttgtcgacgg agtctacctc 83700ctcatctgga ggacatggct gctccggtgc gggaacgact gtttgaggac acttggtgaa 83760cagactggag ctggtctccg atagcaccag tttagacttg gccaagtcgg aggcaaacct 83820tcttctgaga tccatttaag ccttcaaaat tgaacgtgta cgccgaccgc taaatggaag 83880aatcggtggc cgtcgagtac gcggacgagg acgaagatga gattgaggag tacgaggagg 83940aggacgatga cgaggaggaa gagtctgccg agggcgccgc cgcctcctcg gtcagcgacg 84000tagcgctctc tgccgccgag aagctggtgg cctcggaggt cccggacgac gcggctgccg 84060cggacaccaa cgtgcgtcaa cgcgtcaccg cgcgcgtgga ggagcttaag gcgcgctaca 84120cacggcggat gagtctattt gagctcaccg gaattgtagc agagagtttc aatcttctgt 84180gtcgcgggcg gctgccgctc gtggcggacg ccgcagaccc ggcgctcgac aacgagctaa 84240aagtggtggt tcgggagctc gaggagggcg tctgccccat cgtcatcgag aaaaacggcg 84300agttcctctc gccgggcgac ttcgaccccg agtgcctgcg ctaccacctg acgtacatga 84360ccgacctctg gaagtcccag gggcgcatgt agccgcggct acgccgactc ggcggcctcc 84420gcgatttttt cttttatcat gtccagcagc tcgcgcacca cgatggggcg gccgcagtac 84480gtgatgccgt tttcggatat cacgctctgt gcgatgtcca ccagcgagcc ctcgcgctcc 84540cagtactcgc gcgcgagcac ctccttgtac aacgcgcggt ggttggccac gtaccgcacc 84600agcgtctgga tgttcttcac gcccacgctc ttgaggtcct gcggcgagaa cttctcgcgc 84660agcgacacga agacgtcccg gacgagcttg ccgatctcca cgttggtctt gaactcgttg 84720tacagcacca cgtagagctt gcacacgacc gtagcgaact tcgcgggctt gagatccttg 84780ttctggaaga ccagcatgct gctcatcacc ttcttcatga aggccaggta cttcgcgcgg 84840tcgccctcga cgctcacgct cccgacctcg aggtcggaca cgcagcggat tccgtgctcc 84900gcgctctccg cggagacgcg cagcagctcc tggtactcct tgagcttctg cttgtccgtc 84960atcagcgagt tgtcgaatac cgccaccagc ttgagcacgt agttctcgtc cgagaagacc 85020ttgttcagac acttcaccag gaagctgtag tggctctgca ggatcttcat gaccgcgttg 85080gcgccgctgg ctccgcggac gtgcgatatc atctccatga tcttcttaga gtcgtcgatg 85140atctcctcgg tgtcgttgcg catgttgcgg tacattgcgt tcagcgagac cagcgtctgc 85200gcggccagga gcacgtcgcg gaacacgcgc gcgaactcgc gcttctcctc cgcgtcggtg 85260atgctgttgt acacggactt cgccaccgcg ttcgacttca ggaaccagaa ggagagcgcc 85320tggtagttga agtgcttcat cagcgccagc acgtccgcct cgctcatttc cggcgcaatg 85380gggcacaccg agctctcgag cacgggcacc atgctgacga gcgtgtccac gtccgtgtcg 85440aagtccaggc agtccacgca gagcccggtg ccgcggctca ggtgatcgcg gctgatgtcg 85500tagaagcgct cgtagcaggt gcggaggcgg tccatgtcgg ctgcgtttta gagagacaca 85560cactcttgaa ttatggctgc gggtagaact cctgcagcag cgccggcgca cgcgcggagt 85620ccggctccac tcccagcttc agcgcgcagt tcacggacca ggtcttcatg aagcggtcgg 85680gcgcgtccgt gaccacgtgc cggaagagct tcgcgaagtg gcggctcacg gcgttgggca 85740cggtcgcgtt gcgcacgaag gccgtgaagc gcgaggtcag cttcggcgcg aagcgcttgc 85800cgtccacgaa gaagcccgag gtggtgagcg agagcccgtt ctcctcgcgc accacgcgtc 85860gcgcggcctt gtgcggaaac atgctcgcga ggcgcccgct cgcgtcctgg tctaggtgga 85920tggcgtccgt ggccgcgtcc ttgcggatgc gcaccacgtc gtgcacgatc tcctggatga 85980ggatgcgcgt ggccgcggtc tccgccagcc gcatggggaa gtagaccatg tccccggaga 86040tgagcacgtt cccgctagcg tttacgtagc tcactatctc ggacacggtg cgcagacgca 86100cgatcgcgcc ttcgcagcag tgcaccacgt agtacccagc ggtggcgcgc aggcgcttgt 86160tgtccgcctc gaagtccgcc tccaacccct cgttgaagta cttgtcgaat atgatgggca 86220ggaaggatag ttttgactcg gtgaccacct ttccgaagtt gaggatgtac gggttcagcg 86280cgctgcggtc gacctcttcg tcgtacacgc aggacttgaa ggtgtcggtg tgcgcctggc 86340tgcgcaggaa gcagcacgga atgcagatgc gctgcaggcg gtggaagatg gagaggaagc 86400ccacgctgtt gtagcgcccg tcggggtcca tgcacgaaaa catgacgccg tttccgttta 86460cgaagacctc gcgcgtctcg gacttgaaga agttgttgct gaccttggcc atgttcgcgt 86520ccagcgactg cacgatcacg ggcttgcggt tcttggtctt ggtgttctgg cagatgcgcg 86580accagtacac ggtctccacc ttggtgaagt ccgaggactg cttcacgttg ttgaacatca 86640cgctgatggc cacgatcaag aacgtgaagt acttctcgat gttcgggatg tagttcttta 86700ccttcacgga cacgtgcgac ttcgcgagga tgatcgagat gcgcttgtcc gtggagagca 86760ggatgttgtt ggtcgccgtc tccacgaaga tgaagctcgt ttccatgtcc agcttcatct 86820tagacgtgat ggtcgtgttc agcgacacct tgtaggtgat gtcgcccttg acgcggtcca 86880tctttacgtc catgctctcg atgagcttcg tgaacagact cacgtcgttc accgtgagcg 86940tcttcccgtc gctcgagatg accaggtcgc cttccgggcc ccacaccgag aggttcagcg 87000gctcgtccac cagcaggaag cgagtccccg tcatcgacac gaagaagtcg tccgtcttcg 87060agagcaggat gtcgaagtcg cccacctccg ctcgcttgtc cggcgactgc tgcgcgatcg 87120cgcggagccc ggactcgcgc aggttcgtgc ggaagatgtt gttgaacttg gtctccacgt 87180tcatgtttag gtcgaggttc gcgaactcgc ggatgagccg ctcctcgaac ttgaggatgg 87240agtcgttggg ctcctcgaag gagccgaact ccggcgcgga ggtgtccgcc gcgcgcgcca 87300cccagaccac caggaagttg cacgcgtccg cgtacgcgtt gtagaggatg ccgtccgtgc 87360ggatgagcgt tttcttttgc gtgggcgaga acgggttgaa gatggtgttg tccacgtagc 87420tgtactccag gttgttcttg tgcgagtaca cgatgatctc gtcctgcagg cccagcaggc 87480tccccaagta ccccttgagc tgccgcacgc gcatggtcag caagatgtgt ctgcgcacgt 87540gctcggggtc cttctggatg tactgcttcg cgaagaagta gatcggcgag gcctcgtcca 87600cggagtcgta cagcgatagg tacagcacgc gctcgatctc ctggtggcgc cccaccagca 87660ccaccagctg cggcgcgacg gtgtagagca tgttcgcgcg ggcgtattta tagccggcgt 87720taaactgaaa taaaatacgc gggtcgcgag gcagcgccat gttccagccg gtgcccgaca 87780tggccgccga ggccgacatc gacctcggcg acgtcagcgt ggacgcgacg cgcgcgggcg 87840cgcgcgagaa gaccgtcttc ttcgcgcgca acaagcgcat gtacccgcac cgcagcaagg 87900acgaggagcg caagctgtcg ctgggcttct tcttgcagcg gctggacttc ctcacgtcgc 87960gcgaggtcaa cctgcagttc cggtcgctgg acgcgctgcg caccgagaac gtcatgaaga 88020agaacaacgt gctcgtggcg ccgtacatcc tcatcgcgac gctcgcgggg cgcggtttcc 88080gcatgacgga gaccatggtc gagctctact tccccgagct gtaccgcgag accagcaagc 88140gcttccgctt ctgcgcgcag ataaaggtca tccaggactt cctggggttc gcccacgaca 88200gctaccacac ttacgacttc gagacgtact tcgcgttcgt ggcgctggtg ctgcgcggcg 88260cggactctgc ggccgaggcc ttcgacgtcc gcgccgagag cgggcttgtg cgcagcctca 88320ccgagatcac gtaccggctc tacgtgatgc agctgcgctc cgacgccgcg cagtggagcg 88380tgagcaccgg cgccgtagtc tcgcaggcgg tgaacaccgt gctgtcggtc gtcggcgacc 88440tcgctgcgcg cgcggaggcc gagcggctca cgcccgtgtg cgacctcgcg cgcgagaacc 88500cgctctcgct cgaggacctg cgcaagtacg gcccgcggct gcgctcgctg ctcacgacca 88560tggcgcgcgc gcgatccttc aagacgaacc ggcgggacaa ggacgcgctg tcccggttct 88620gccgactgac ggcgggccct agcccgtctg cgtgccgcgc gtcgccatag gcgtcggcgc 88680gcgctcgccg ccggaacact cggggtcgct gaacatgtag atgagcgcga cgcctagcag 88740caggtacatg atcatgctga tcacggtttt gaacacgacg gcggcgaacg tgttggaccg 88800cagtcggtgc tcgcagaagt gcatgaacag gtgccgcatg aggtcgatgg ccccgttggc 88860cacctggaaa agggcgaggc cgccgatgga cttgatcacc gtcacgtagc acggccgcat 88920tccgacgacg ctatttactc actgtcaaaa gaaacggcgc catccgaccg gaggttgagg 88980ttgcgcttca tgttgttcca gtacatctca ccgatgctcg agtagtacgc cgtcagccgc 89040gatatttttt ctcgcaccag ctcgtaggcc ttctgcatct ccgcaacgcc gatctccgcg 89100tcgcccacgt accggccgct gcggcgcacg atcagcagca gcgccttcag gttctccagc 89160gcgatcatgt ccatgtacag cgacttcgag agctgcacga agaggttgta ccgctccagg 89220atgctgttct tcacctcgtc cgcgatcggg actccgaaga tgcgctccgt ggtgtacacg 89280gactgcgtga gctgcttgaa gagcgcggag atgcagcagg tcgcgcgctt gacggcgtcg 89340agctgcttct cggagcgcgc gctcgcgatg ctcagcgcgc tgttcacgac gttgctcgtg 89400tcgcgcacgt agcgcgtctt cagcgcggcg ttgatggcat ccgcgatctc gttgctgctc 89460acgctcgagt cgtccgagct gcccgagacc tcgtccagca gccccgagat cgtgatgtcg 89520ggcgagccgc cgacggtcac cagacggtcg agcaggttgc agggcatgga catgaggatg 89580ccctcgctcg agaggcagcc ctcgtcgatc atgctctgca ggttgcgctt gaaggccgtg 89640ttttcgggca tgaacccgtc cacgctcatg agctcgtcga ccgtgctcgc ggaaaagatg 89700cccctcacgt tgatgcggtc cagcatgccc atgtcctgcg agcacagcac caccgactgg 89760tcggccgtct cggcggcgtc cttggcgccg ccgtagatga tgcgcggaaa ccgccagctc 89820gccggaaaag agaaggaggg aaaccggcac tgcgcgctcg ggcctcggta gccctgcgcg 89880tcgcgcacgt tggtggccgt gaccatgaac tgcagcaggt cgtgcgcgga cgccatgatc 89940ttctccacct cctccttgct gcagcagacc ttgcccaggc tgcgcgcgat gttcgttttg 90000ctcactgagg gcgagaccgt gacggcggtg tgtcggcggc tgccgagcgt gtacgcgctc 90060acgctaacgc ggtaccccat ggcgccgaag agcagcttca cgaagtccag gtagctctcc 90120ttattgatgt agtgcggcgc gcccttgtct tccatcctca gcccggcgta ggccatgagc 90180acttccttca tcgccgtctc ggggtccgag ttgcacacca gccgcagcat ctggaagaac 90240tgcatgaagg cgcgctgcga gagcccgatg tggtggttgg gctgcgtcga ccggcgcggg

90300aactccctgg gcgtcatggc gttgatgccc gagagcgtct ccatcacgag cgcgcccacg 90360gtcttctggc ccatgacgcg cgggtaaaag cacacgcgga ggggctcctt gccggccgcg 90420agcgcgtccg agagcagcga gcagtacgtg acgttgtcgt ggtcgaagag cgcgaaggtg 90480tagcagacgg agctcatgaa gagcgagtcg gcggtgctca tggacttgaa ctccgtgtac 90540gcgattccgt cccagaacag gctctttccg ggcgcgatca gcggggacgc gcggtcggcg 90600cgcatcagca tggagagcag cgtcacgtag taacggatgt tggcggaaat gtctacgaac 90660tgcatgccgg gcgaggccac gcgcagggtc gcgcccgagg tagtgagcac ctccaggctg 90720tccatgagcg tcacgctggg gtgcagctgc gcaaggcgcg ccagctggct ctggtagaag 90780atggacacgg cgaggctggc cacgctgccg cgtgccatgc gcaggttttg cccgttgaag 90840gtgagctggc gcagcgagaa cacggagtcg aagtactgga agaaggtgag caggtacttg 90900agcggcatgg tcgtcagctc ggtatccacc tgcggcgtct gtgcgagcac gattccgttc 90960ttggccgcgg cgtcggggat gtcgtacatg gcgtccattc tggcgcggga ggcgtcggtg 91020agcagcgcgc gcacgttgag cagcatgagc aggtcccgcg ctagcatggt cccgtcgacc 91080agccgggcgc gaaagccgat ctcggcgggg ccggcgatgt tggggtagat caggttcagc 91140aggtacgtgt tgtcgaagct cagcgagggg aaggagatgg gagacttcgc cgggaggccg 91200gtggggtagc gcacgtagcc gccgcagatg cgcgcgtgcg cctcaaagct ggtcacgcga 91260gtcttcagca ggttgcgggt gaagggcggc acgtccttga aggactgcgt gcagatcacg 91320gggttggcgg tgtcggtcag cttgaggttg gtgggcttaa gctccgcgaa gttggggccc 91380agcagcacgg ggacgaggtg cgagttggcg gcgctgtcga gcaggaagtt gatgccgaac 91440tgcttcacgg cgacctcggt ttcctcgtcg ctggcgagct tctccgcgtc ctcgaggaag 91500agcgcgtcca gcgggtgcac gtacgtgcgg ttgacgtcgt agctgggctt gaagtccgaa 91560cacagcgtgg ggagcacggt ggagacgagc tggaacatgt attccgcgcc ctccacatgg 91620tgcaaggcca tgtgcacgtt tggggccgtc atttatttag tattaaatga cggccgtacc 91680ggtaaccgat attcctggag actacgggcc gacgtccttt tcggaggaca actacccgct 91740gaacaagcac tacgagctca ccaaaggcca gctctcgatc ctgcgcacgg tcaacgacaa 91800gctgctcgcg cgcaccgtgc agcactcgga cggggagagc gatgagagcg aaagcgagga 91860ggacgacatc tccagtccgc tgccgccgga cgaggaggag ccggactcgt gcgtggcccg 91920ggtcatgccg cgggacgcgg acctggcggc gccaaaaaag gccgacggct acatcattgc 91980cgccgagcag cagcgccagc agcgcataaa cattctggta tccgatcgag aggccgtcgt 92040ggagcgggag ccggttcaga cgtcgttcgc gcgcgtctcg gctatcccga tccacgggga 92100cggcgcgcgc cgcaccaccg cctccttctc cgcgaccacg ccgtcgctgg gcgccgtgtt 92160cgacgacgcc aagcgcgtgc ggctgctgga ggaggaggtc aaggagctcc gcagaaagtg 92220cgcgacctct caggataacg gaaacctgga gaacttcacc aaggtgctgt tcggcaaggc 92280gccgcgcgcg agcgagctga acaagcgcgt ggtcatcgtg aactacgcca cgctgaacaa 92340cgtgacgctg tccatggagg acctcgagaa gtgctccgac gaggaagtgg accgcatgta 92400ctcggtcatc cggcgctaca acgagacgcg gaagaagaag atcctggtca cgaacgtggt 92460catcatcggg atcaccgtgc tcgagcacgt gctggtgaag cttggcttct cggaggtgcg 92520cgggctcagc gccgacctct cgtcggagct catcgacgtg gagatcggcg aggactgcga 92580gcacatcgcg gagcgcctgg ggttcgggaa cagcccggtg ctaaacgtgg cgctcttcgt 92640ggtaaagctg ttcgtgcgga agctgaacct gatctgatca acacatgccg ccgtcgaggt 92700ccatggcgtt catgaggttg gaggcgcggc ggcgcgcgcc ggtggaagcg gtggaggcgc 92760tcgaggtcgt ggagcaggga gtgttgctgg aggaggcgcg gcggcgggag ctagaagcgg 92820aactcgaggt tccgctggtg gtgctgcggc gactcgtgcc gctcgtgccg ctcctgctag 92880tgccagtgcc agtgccgctg cggcgtgaag taccggtgcc ggacctgccg ctggagcttt 92940tcttgcggcc gccgttaacg ctgtcgatgc cgagcaggtc ctcgcacacc tcgccgacgg 93000ttccctgcac gtccaacttg ccgttcttga caaccccgta cacgatcttg ccgcagttgg 93060acacagcctg gatggtggtc tcgtcgctgt caaaggcgtt cattccgccg cacccgccgt 93120cgttgtttct tcgagaaggc gcgccgctgc ggcgactcct ggtgctgccg ctggaccgag 93180ttccggagga cctggagccc gtggaccggc tgccggtcga cctggtgccg gtagtgcgct 93240ttctggacga agaggaggag gcgcttccgc ggcgggtgga cgaactagcc tccagcgcac 93300cggcgccgcc cacacaatcc acgtcggcgg cggcgcctcc gcgaatgacc tgctcgttgt 93360tgagctgcgt caggagagat cgcagctgcg gcgcgatctt ctgcaaggtg ctcacgtagt 93420cgtcgtagct gctctgcggg cgctgcgcca ttttttcgga cgccatttat tacgcggaat 93480atctacgacg acgcagcact gaatcggttt ctcgcgacgg gagattccgc ggtcggcgcc 93540ggtgcggggt tgtcaccggg cgacgaggta accagcgcgt ggaaggcgcg cacctggtcg 93600tccgtcatct tgtcctcgaa cgaggacgcg cccgggggga gcaggtcctt gttgcgcgga 93660acggcgggcg ccgagacgca cgaccggcgg tacatcatga tgacgatgta gcacacgatc 93720gagatgacga tcacggtcag cagcgcgtcg aggagcccca tttattacct gtatatgccc 93780gcgtttaccg ggcggtgagc tcaatgtcgg tgttgtttag ccgggcgtac gggacgctgc 93840cggagcactt cctgtacatg ctgaacacga acagcccgag cagcagcacg gcgcccacta 93900tgaagcaggt tacgcacagc gcgcgccaca cgtagtcggt gacgttgttg gtgttcttgc 93960tgaaatccac gaaggcgaag acgcaggcgg ccgtcagcag cagcacgccg catatcagca 94020ctccggagta gtaagagctc aaggtctcga atatgtccat ttatctgagg agaaatttaa 94080attactgaat ggacgaagtg gaatagaaac cacgagaaca cgacggactg cagcacgaag 94140atggtgctca gcttcgtctt catgggcatg cagaagttcg cggccagcgc catacagaag 94200atgaacacga gcaccgccgg gtcgtagtcg gacaccattt acactacgct aaaaggcata 94260tctcggcgcg cgacgtccac gagcaccagc acgcggacgc ccgcgggcgc gccggcggcg 94320accgcggcga gctgcccggc cgtggggttc accagcagca gtgcgcgcgc ggttcgcggg 94380acggggtctt cgtaggccat ggtcggcgtg gacccgggac gcagcggccg cccctgtctg 94440tcgaagaggc cctcgggaaa cgaggtgccc ggaacggcca cgacgacggt gtcgctatct 94500agaaacattt atggtcttgg tttccacgga tcgcctcgag tagaccgcca cgaagtagaa 94560gatgacgccc gccgcgagcg ccgccaccag gaagggcggc acggcgggca ggttcgcgga 94620cgcgttgtcg cgcacgccgg ggtccgggtc tgcgtagccc gcgcccacgg ccttgccgca 94680gtcggcgatc atgtgcgcgc gcgagttctg catgaccagg ctgtccacgt cgatgcggca 94740gcccacgtag cggcaccgcg agcgctgctc gtcctggctg aagaagagcc acttgcggtc 94800gcgcgactgg tccgtgcact cgtgcgcgcg acagacgcgc gggcccaggt acttcccgag 94860cgtggtgccc gcgacacacg cgcactccgg cgcggcgcgg tgcgcgtcgc agtagcgccg 94920cagcgcggag tcgccgaagg cgaaggaggc gggccgcgcc acgcgcacga actccgagca 94980gaagcgcgcg tccatgtgct tggcgcagag cgccgcgtag gtgtccagcg ccgcgtagcg 95040gcccgtgcgc agccaggcca tgcactcggg cgcgtcaggc tccaccgcgc agcggctggc 95100cataacgccg tcgcagtgcg cggtcttgta cccgttcgcg aacacggacg ggcacccggg 95160ctccggattt gtgcagcagc gcgccatggc ggcgtccgtg ggcggcgccg aggcgccgat 95220ctcgaacgcg cacatggtgc cctggcgcag gtacggcttc gcgatctcgg gaacgtagtc 95280tgcgcgcagc agcgagcccg ggcggaagaa gagcgagtcg cagggcgggc ctcgcacgag 95340ccgcgcgcgg ctcgccagct ctggcgagag gaagcgcccg cactgcccgg ggtccatggt 95400cggcagcaga cagaaccgcg gccgtacggt cttcagcttc gggtcggaga aggtttctga 95460ttcttccgcg aaggcgaagg tgtccgtggc gctcgtgtgt gtgacgcgca gcgcgtactc 95520gcctggcgtc ggcgtgtcga gcacctccac cttggatacg gtgtccccca tttgaagacg 95580ctatttacgc cgctgcctac tcggcgaaga ataggtcctc cgacttggcg cccgcgtaca 95640ccgggcaggc gggcgcggcg gagcgagtgc gcacgatacc gcggccagtg aggcggaagg 95700cgtagatggc gaacagcagg ccgagcacga tgtacatgaa agtggtggcg cccacagacc 95760cggtcacgtg cgtcacgatg atggtgacga tggacatgat cgtgcacacg atggccatgc 95820cggtgttgtt ggccgcgtag gggtgcatga tctgcatggc cgcacagtat ccgatgacca 95880ggcacggcag cgggaggata agtgaggcaa tacctatcat tactagagcg agcacggggg 95940tggacgtcaa ggccaataca aaaatcacaa tacctgttag tatgcggata tcctcgtact 96000ggaggacgct gtaaggcgcg atattccctc cgggcactgg cctggggtta gccgggacta 96060ggggggagtc ggcagtgccg gggtccttgg ggagaaaggc attctgctcc tccgggctga 96120agagctcggc gtcctgaacg ccgccggcgg tgaactcgtc gttatagtaa ctaaagtagc 96180tttccattta tatgttgaaa aatgtttgga ggcgtacagg tggacgacaa actctacgcg 96240tacctaaaaa aactcgccgg acgcgggcgg ccgctgtgtc tgttccgcga caacggcgag 96300ttcgtcgaag tcttcgcggg gtccgcgttc cgctttgtgc tgcccgtggg cctcttcgcg 96360gacctgcgcg tgcgcacgcg cggcgtggcc ttcccgaaac tgcgcgactc cgcgcgcatg 96420cgcggcgtgc gggtggacgc gcacacgctg ccctcgctgt accccaacca gcgcatcgtg 96480gtggacgagg tgctcgcggc ccgcgaccag ttgctggccg cgggccgcgc cgtgtacgtg 96540acgctgcatc tggcttgcgg cttcgggaag acgctgaccg cgtgccacct catcgccacg 96600cacggccgcc gcgcggtggt gtgcgtgccc aaccgcatgc tggtgccgca gtggcgcgcg 96660gccgtggcgg agctgcgggt gcccttcgcg gtctcctgtg acggcgcggc ctcgctgctg 96720cgctcgggcg agctcgaccg cgccatggtg gccatcgtgg tcagccggca cttcgccaac 96780gacgacttct gccgcgcggt gagccggcag tttgacgtgc tcgtgctcga cgagtcgcac 96840acatacaacc tcatgaacaa caccgcggtc tcgcgcttct tgaccaagta cccgccgccc 96900atgtgcttct tcctgaccgc gacgccgcgc acggccaacc gcatctactg caaccgcgtg 96960gtgaacgtgt ccgtggtcag ccgcctcacc aaggtagtgc gcgtggtgga cgccttcttc 97020gagccgtaca ccacgcccaa gatccgcacg ctcgagcgca gcctcgaggg accccagaac 97080aagtaccacg tcttcaccga gaagatcctc ggcgaggacg tgcaccgcaa caagctcatc 97140gtggacaccg tggtcgcggc catggccgcg ggcgaggcgc ggcgcgtgct cgtgctcacc 97200aagctgcgcg aacacatggt cgggctgcac gccgcgctct gcgagcgcct cggtgcggag 97260acggtctttc tcggcgacgc caagaacagg aagacgcccg aggtcacgcg cgcactgcgc 97320gacaaggacc gcttcgtgct cgtgtccacg gtcttcttct cgggcacggg cctggacctg 97380cccaacctgg acgcgctcgc ggtggccgcg gccgtgctca accgcatggt catggagcag 97440atgatcggac gcgtgtgtcg cgagtcgcac gccaacacgc gcacgctgtt cgtgttcccg 97500gactcctccg tgcgcgcgat ccgcgacacc gtgtctgcgt ttgcgcagcg gctcgtggcg 97560ctggcggtgg acgggctggg cttcgtccgc gagcgcgccg ccgccggcgc gaagaacgag 97620ccggcgctgt acagcgccat cagcgggcga gatctcgcag cggtgtaagc gcggacccgc 97680acgccgcgca cgagagcgtg ctggagcagg cgagtcccag cgacagtgtg gacagcctgt 97740ccacgtcctt gatgctcacc agccgcgagt tgcacgacga gcacacgggg tcgctactat 97800catcgaccac cgtggtgacg cggcggcgtc tgcgcttttt gtttccagcg gcgacatcga 97860ccacgcctcc cttagagccc cccttcgccc ccgccttagc tttcaccgcg ctcatctttt 97920atttatcata aaaacacgtc tgcgtacgcg ttcgcgcaca cgtcccgcaa atccgcgcgc 97980gcgccgcagc gcgtgaagcg cgcggcgtcc gcctccgcga tccgcgcgca cggcagcggc 98040gcgcccttct cgtccgccat cacgcgcgca gagatcccgg tggcccccag cgcgtacgac 98100accaccacgt cgccgacgca gcggtacacg ttgccggagc cggcgaggcg gtcgaacgcg 98160gcgccctcct ggcgcagctt gtcgaatatg cgaggaacga ggatgttaaa aatgagaacg 98220aaatagcaga tcagcaaaaa cagcgagatc atgacctccg agagcgattt atataccttg 98280aaagagctaa tacgacttcg ggactcgctg cacctcgcca ccggcgccgc cgtcgagcgc 98340tacaacgcgc tcgtggagtg ggccgcgcgc acgtactgga cggtcgcggt gctgccctcc 98400gcgccgtgcg cctccatcga gaagtactac tgcgtgtgca aacccgactg cgcgctcgag 98460cccggcgagt actccgtgag ccggctgcac ttcggactca cgcacgcctg ggtgcgcggc 98520gccgccttca actcggccag cggcgccgag gtcgagccgc cagaggaggt gcgtagggcc 98580tgcgaggcgc tcgacgccgc cttcgcggac ctcaccttcg tgcgcttctc ggtcttcggc 98640cgcgagtgga cggtcgacga cgccgtcaca gaccactcct cgcgcgacga ggtgctcgcc 98700gcgtgcgccg cctccggcgt gcgcgtcgcg cgcacgctgc gtgtgcgcgt gcgggcggga 98760gagtccttcg cgcgcgcaga cttcgacgcg gtgcacgccg cgctgcgcgc ggagggcgac 98820gtcgctcgcg gcaccgcggt ctgtctcgcg ctgcgcgggt catcgcgccg ctggatagcg 98880gaccgcgcgc ctcgatgctt catgcgcgtg cgccgcgtgg agctcgagcc cgtggacgcg 98940cggcaccact gcccggtgct gatctccgcg cgcggcgacc gggtgctctg ccgcggcgtg 99000gggcacctcg cggacgcgcg cgcgcgcgag ggcgtcttcg tggccgtgcg caggtacccg 99060gagtgtctgg tgctctgcga cgaggcggcc gccggcgcgg cggagtgctc gcgcgaggag 99120gcgctgcggc tgctggtgcg ccgcttcggg cgcgacttcg ccgtcagcga ggagggctac 99180gtcttccgcg tgcaggacat ggacttgcgc ggcgtgtccg cgcgactggg gctcgcgccc 99240tgcgcgagcc tggaggagct gcgccgagcg gtggagcgcg accgcgcgct gatgaggcgg 99300ctgcgcgcgg agggcgccgt gcgcctcgcg tgcgagtgcg tgggataccc gcgccagaac 99360gcggtggagc tcataaataa tatgcgcttt caaataacgg aagaaggcgc ggtggcgaac 99420tttgagctgg cgaacgcgag ctgtctcggc aacccgaccg cggagtccat cttcgcgagc 99480ttcgcgcagt tcgtgccggt cttcaacgtg ctctcggcga tcgcgcgcgc gcagccatga 99540tcgtggcggc cttcgacctt ggcacgcgca accccgcgcg caccgtgctg gaggtgctcg 99600acggcacggt gcgcgtggtg gacgtggcca agctggactg gagccgcgac tgggagaagc 99660gcgtgcaccg cgacgtgacc gccttccccg cgaacgtggt gctcgtggag cgccagtgca 99720agatgtcgcc tttttctaag ttcatatact tcatacgcgg gctgctctac gacgggcggc 99780gccgcacgcg cgtgctcgcg gtgccgccgg ccatgaccgg cagcacctac cggcagcgca 99840agcgccgctc ggtgcgcacc ttcctcgcgc tcgcggagag cttcggcatc ctggacgccg 99900tgcccgcgcg gaagaagctc gacgacgtcg cggacagctt caacatggcc atcaattacg 99960tgctccgaac aaactgaaat acgactggaa cgaataagtc atgctggcgc tgttcgagtt 100020cctgcggtcc gtggaggact gctaccggcg caccatcttc aacttccaca tcgcgcacag 100080cgccgaggcg ggcgatgtct acggcgtgct gcgcgaccgc attttggcgg ccacgcgctt 100140cgaggaggta gcgccgccag ggctcgcgga cgcgctggcc aaggtggtct actgcgacat 100200aagcaccacc aagcacctgg tcaaccacgc ggccttcgcg gcgcgcgcgc ggccggcgcg 100260gcgcggaggc agcctcgcgc agttcttcga cgtgcacgtg ggcgaggacg cggagagccg 100320ccgcaccgcg gagatcttcg accgcgagcg ctcctcgctg gtctcgtacg tgaagaccac 100380ggccaagcgc tgcaagatcg actacggcga gatcaagcgc accatccacg gcgggcggca 100440gacctacttc tcggggcggc gctcggacga cttcctgagc accaccgtgc gcgcggaccc 100500gagcaagccc tggatcaagt ccatctccaa gcagctgcgc gtggacatcc tgcaccacgc 100560gatctgcacg cgcggcaaga gctccatcct gcagaccatc gagatcgtgc tcacgaaccg 100620cacctgcgtg aagatattca aggactcgac catgcacata atcctctcca aggacgaccg 100680cgagcgcggg ctcgcggacc tcgcggacaa gctcttcggg acctacgcga ccaccttccg 100740cgtcatcgcg gccatcaccg gcaacgcctg cttcgcggcg gtggcggacg cggccgcgcg 100800cgtggtcgcg ctcccggacg cggacgcgaa gctggcggcg gtgcgcgggc tcgcggagtg 100860ctacggcgtg cgcaacttca aaatcggcat gttcaacctc accttcacgg gcgccatcga 100920gcacacggtc ttcccctcgc tgatccccgc ggagagcaag atcaagttct tcaagggcaa 100980gaagcttaac atcgtcgcgg tgcgctccac cgaggagggc cgcgagtgcg tggagcaggc 101040gcaggcgctg ctcgcggcca tgcgcgagcg ctccgcgcgg ctcgcggccg cggacgtggc 101100caccgcgagc gtggacttcc tcaaggagct gctggggcca tagtgaaata atactgattt 101160cttaaatatg gagcaggcgc tcggatacaa gtttttgttg cccgacccca aggacgacgt 101220ctactaccgc ccgctccact tccagtatga gtcatacgcc aacttcatca agcaccggct 101280taaggacatc ctcacggtgc ggcgcacgct gctcaccttc aagaacggca ccgagtccat 101340cgtgctcgag atcgacgacg tgaagatctc ggcgccggag ttctcgccca tcgtggccag 101400catcaagggc cacagctacg aggcgctggt caccttcacg gtgaacatct accggcacgt 101460gatgaccaag gacggcctca ccgttaccaa gatcaacagc tacgagggca ccgactcgca 101520cctcgtcaag ctcccgctgc tcatcggcta cgggaacaag aacgcgctgg acccctccaa 101580gttcgtggtc ccgaacgcca tcggcggcgt cttcatcaac aagcagtcca tcgagaagct 101640cggcatcaac atgatcgaga agatcaccac ctggcccaag ttccgcgccg tgaaggccaa 101700ctccttcacg ctctccttct cctcgatctc gcccgtgcac gtgatgcccg cgcggtaccg 101760acactacaag atcctgctcg acgtgaacca gcccgacaac ttcgtgatct cctccgcgaa 101820gaccttcatc accgtgaacg tgatcgtgat ggtgcagttc ctcgcggacg tcacgctcga 101880gttcgtggcg cgcaacctct gcttcgacat gccgcccgag gccgcgcacc tggccaccgc 101940gctcgtggag agcgcgaaga ccgtgcccgc gggcgcggac gtggccgagt acgtgaacgc 102000gctcatcgcg gccgagcacg cgaagcagaa gtcgacgctg tccaaggagg agttccgcta 102060cgagatgctc agcaacttcc tcccgcacat gcaggacagc gccaaccagc tcaagggcct 102120gtacctgctc tcgctggtgc gcaagatggt cttctgcgtg ttcttcccga accggtaccc 102180ggaccgcgac tcgctggtct gccaccgcgt gtacacctac gggcgctact tcgaggcgct 102240ggccatggac gagctcgaga cctacatcgg gaacatccgc aacgacatcc tcgcgaacca 102300caagaaccgc ggcacctgca ccgtgaacat ccacgtgctg accacgcccg gcttcaacca 102360cgccttcgcg gcgctgctca gcggcaagtt ccgcaagtcc gacggcagct tccgcacgca 102420cccgcactac tcctggatgc agagcatctc catcccgcgc agcgtgggct tctaccccga 102480gcaggtcaag atctcgaaga tgttcaaggt gcgcatgtac caccccagcc agtacggctt 102540cttctgcgcc tcggacgtgc ccgagcgcgg gccgcaggtc gggctcatct cgcagctctc 102600cgtgctcgcc tccatctcga acatccgcac cgcggacttc gtcgagctca ccaagcgcgt 102660ctgcgactac gtgcgctcct accccgcgcg cgacatcagc tacttcgaga ccgggttcgc 102720ggtcaccgtc gagaacgcgc tcgtggcctc gctgaacccc gcgatcgtgg acgcgttcgt 102780gctcgacctg cgccggcgca agcggctcgg cttcttcggg aaccgcgaga tcggcgtcgc 102840gctcgtgcgc gaccgcatga acgaggtgcg catcaacttc ggcgcgggcc ggctcatccg 102900cccgctgctc gtggtcgaga acggcgtgct cgtcatggac gcggaggcgg agcggctcga 102960gcgcgacctc gccgcgatga ccttctcgga cgtgctgcgc gagttcccgc acgtgatcga 103020gatcgtggac gtggagcagt tcagcttcag caacgtctgc gactccgtgc agcgcttccg 103080cacgctgccg cccgaggagc gcgcgctctt cgacttctgc gacttcccgg ccgagttccg 103140cgacgggtac gtggcctcct cgctcgtggg catcaaccac aactccgcgc cgcgcgccat 103200cctcggctgc gcgcaggcca agcaggccat ctcctgcctg agcgcggacc tgcgcaacaa 103260ggtcgacaac ggcatccacc tcatgttcgc ggagcggccc atcgtggtca gcaaggcgct 103320ggagacctcc aagatcgcgg acaactgctt cgggcaccac gtcaccatcg cgctcatgtc 103380cttccgcggc atgaaccagg aggacggcat catcctgaag cggcagttcg cggagcgcgg 103440cgggctcgac atcctcacct gcaagaagta ccaggtcgag atcccgctcg agaacttcaa 103500caaccgcgag cgcgtgcgct ccgcggcgta ctccaagatc gacgtcaacg gcgtggtgcg 103560cctgaacgcc ttcctcgagc agggcgacgc catcgcgcgg aacgtgtcct cgcgcacgct 103620cgacgacgac ttcgtcgccg acaaccagat cagcttcgac atcgcggagc ggtactcgga 103680catctacgcc gcgcgcgtgg agcgcgtgca ggccgacctc accgacaagg tcaaggtgcg 103740cgcgctgacc gtgcgcgagc gccgcgccat cctcggggac aagttcacca cgcgcaccag 103800ccagaagggc acggtcgcgt acgtggccga cgagactgag ctgccctacg acgagaacgg 103860gatcgcgccg gacgtgatca tcaactcgac ctccatcttc tcgcggaaga cgctctccat 103920gctcatggag gtcatcctca ccacggccta cgggcacaag cccttcgccg aggacggctc 103980caaccgcccg atctgcttcc ccagcaccaa cgagaccgac ttcgagacct acatcgagtt 104040cgcgcggcgc tgctacgcgc tctcgcaccc cgaggccgcc gcggacgacc ccgagttcga 104100gcaccgcgtc ttctgcgagc gcgtgctctt cgaccccgag accgacgagc ccttcgcggc 104160gcgcgtcttc ttcgggccgc tgtactacct gcgtctgcgg cacctcacgc tggacaaggc 104220cacggtgcgc tgccgcgggc gcaagaccaa gctcatccgg caggccaacg agggccgccg 104280ccgcggcggc ggcatcaaga tcggcgagat ggagcgcgac tgcatgatct cgcacggcgc 104340ggccttcacc gtcgccgaga tcctgcgcga ctccgaggag gacgcgcagg aggtgctcgt 104400ctgcgagaac tgcggcgaca tcgcggcgcg gctcaacggc acgcacgtct gcatccgctg 104460ctccaagatg agcctctcgc cggtgctcac gcgcatggac tccacgcacg tgagcaaggt 104520cttcaccacg cagatgaacg cgcgcggcat aaagatccgc gtggagttcg agaagcagga 104580cccctgcttc tacgggactc cgaaacggtt cagcctcgcg cccgacgagt cgctgttctc 104640gccggaggac tgaacccgcc gtcgcgaccg cgtcgcgacg actagcttat cgttcgactg 104700atgcgaaacg cgcggcggcg ccgcgactta gcttatctcg actgatgcga acgcgcgacc 104760tctcgcgact ttctagcttc tcagactgat gctaccatat cgcggcgtgc tggccccacc 104820accagggctt ctcgccgtgg ctgacgcggg gctggctgcg acgcgcgctg cagtagctgc 104880gcgcgcccca gtcgccgcgc acgtgcgccg ggggcaggct cccgtccagc gcgtgccgcg 104940tcacctcggc gccgggccgg cggcacgtgt gcacgtccgt cttgttggag acgagcaccg 105000cgtactgccg catggtctct atgtgatgct ccaagtgctt gcccgccatc cggttggact 105060cgcagcacgt ttttgcttcg gctaaggttt tttctagagg ggatagtagc ttatccacgc 105120gctcgggcag gacgcacgcg gagccgtcga accctacttt gaacggggtc accttgatgt 105180tcccgtcgta gcggtcccac agcatcctga ggtaggttgt accgtcgggg tctgggtctg 105240tccacactct aagcttttcg ctacagcggc cgtcgtacgt aagacggtct ctacgctcgt 105300agtagtttct gcttatgttg ttggggtctc catgctcgta gtagtataaa tcgtacgcgc

105360ctggcttttt taagtcgttt tcgtcgttgc tgacgtgtat cacgtcggga taataggata 105420tcctaactgc actacaatct atagtatttg gtctagtaag ctgttcgaga tcaccttgtt 105480catcatgatc tactgatttg tacacggcac cgtcgtgttc cgacggacgt atgaatatgt 105540ccatggtaaa cgatgtaccc actttggaaa acgtatccca tgcagtaaag catagtccgt 105600ccattataaa ctcaggaaca ctcataacaa atcgaaatct gtgaagtttt tcgaacacca 105660cttttacatg gtctttgtca cgaacatcat tgccgtttac ttcagacatg aattgaagga 105720acgctaaaga gtttcttgtt tcttcatgaa tctttccatt atacgtccat ccagtttcta 105780gaattctata tatgcttttt gcatcgaccc cgtaccacca gtacatggga actccgaaat 105840atatagctgg gtttgagtac caatgggcaa gagtgcccat tgcgtttaaa aagtcttgac 105900aaaaaaatgc agtttttctg tcgataactt gacttggact acgttcgtgg acatcgtaca 105960tgtccataat tggttcattg gtaacggtta catgacccgt cattatcttt ttaacaatca 106020taagatacag tttgcctaaa gtcgaaatat gtataacgtt aatttttaca tgttctccta 106080acgtaattgc gtttttactt agccattcgt cgtctacaaa aatcttacga tacataggat 106140ttctctctac gtatcttcta aagtatagat ttaccggtct accggcgaca ttagcgccat 106200ctatagcagg agcaagctgt atgtatcgtc gtataatgtc tcgtataagc tttctgtctt 106260ctctgggaat acacgacacg gaacttagag actggtgcca gtgtctttca accaaagact 106320tgaacctagc aaccaacgcg ttgtcactct ccatttataa ttaaataatt atcccaactt 106380cgtatgttaa tccttattac cagatagcac cgctccttcc tctccaccac gtactatcta 106440aaggatacct gtaagggtaa tgtctggata acgggcgtgt gagccaagac gtgttatggt 106500gtcttcccca ccaacggtcc acttctctaa ctaccggagt gctagacgtt gtcgatccca 106560ctactgttgt ttctccatta cctgtaatct ttgaagcgca acaagtgtta gtctttgcca 106620aatcttctaa cggcttaatt aggtcgttaa gtctgtcaat atccatgcac tgaggtgtat 106680cgttaccagt ttccccaact ttgggaggaa cttcctgttt agtgtccaaa taacccataa 106740acctgtctct aagcattctt ctgtacgtgc ttttgtcatt gtctatgtct cccaaaaacc 106800tgtgatttac gcatccgttg tacgtaagtc tgtcccttcg ctctcgctcg ttatttccta 106860cttcttctat tgtactgtaa tgttgatagt ccaagtaata gccactgttt tcatgatttc 106920ttgtaaatat aatcggtgtt ttattattga catcgtgttg cctactgtac gtatcttcca 106980tggatctagg aacttgtcta gaaaattgag gactagaaat acgccttcca aatcctggat 107040gataaaccaa acgcaatgca ctacagtcga catcgtcact gtctctagtt atcccatcaa 107100caactccttc tttgtgatgc tccactgttt taaagttaac tcctctgtat atgttgagtg 107160ttaaaaaaat atccacattg aacgcagttc catattttgt tattttatcc cacgacgtat 107220aacacaaacc atccataatg aattccggaa ccgatactac aaaattagta tcgaacttat 107280caaacgaaat gtttactcgc ggttgtgctt cgtatttttt gtcatacaca tctgtcatgt 107340attgtacaaa atctatagct cctctagcat cgggcatgct aacgtctgga aaatcatttt 107400ttaactgttc tagtacgaac tgttgatttt ggtcatctct ccaccagtac attggcaatc 107460ctatcacaag agacttattt ttataataat ttgctacaga agctatatgc cacatgaaat 107520tatagcaaaa ataatccatc tgtatgttta aaaccggttt actagtctgc tgagagtagc 107580tatccatgat agtgtttccc tcgccaatac ttcctgacat tattctgtaa acaaccatta 107640acaaaaatct tcctaccgtt gttatgttgt caaaagttct tatgttttga gcactttcga 107700gtaaccatat gttattttca aatatacttt tgtaaactgg atttctgtcc acataactct 107760ttaaaaatag atttactgga aggccgcttg ggttatctcc ctgtataggc ggcgcttgct 107820ttatgtattc gcgtaacaaa tctcttacaa cttttctagt tcttctaggt atacatgacc 107880tattattcaa aggctttgtc cagttagcgt ttataaacgt tgtaaagtca ctgactagct 107940tctccattta taattaaata attacagacg gcaacacagc ggttatctaa tatctgctgt 108000atcctgtctg tacatctatt tttctgttga gatcaagaag agctttacgt agactctcca 108060agtgtctttc tagtctgtct aaccggttac ctgtttctct gcagcaatca gttatagttt 108120tgtaactgtc taacaagctt acgaggcgct cttccacact ttctttagtt ggagctccag 108180ccgcgtacac tccgttggtt gaattgcctg tatcatcagg ctgagtcaat aggttttctc 108240cgtcattttc atccatattg agtccaacga acacaaacga gtaagtgttc ctctatttaa 108300agtattgatt ttagaaaaag gcaagcctcg ctgccctgat tcggcggcaa acacgggttg 108360aacacgcgga agtcgctcgc ggccgtgaag atctcgtccg cgcacgcctc cacgctcgcg 108420aagcgcccag gcgagacgcc gtcgtgcgag cggaacccga actccgaggc cgccaccgcc 108480gcgcccttga agagcacgca gcgccacttc ttgcgcacgt cgaaggcctc gtcgttgggg 108540tcgaacacgc gccggtccac gcgcgggccg cccgccgtgc gcgcgaactc cagcgccgcg 108600ttcgccgcgt tgaactcgcg gatgttgtcg tagttctcgt agacggccca gagctgcagc 108660gccacgaaca tggcggccgc cgccgcgagg gccacgcaga gcgcggacac cgcgtccatc 108720ttttatgtgc agaattattc gttggcgcgg agctcgcgca gctccgcggc gcgcagccgc 108780gcgaaggctg ctttgagcgc gcgcagcagc tcctcggtgt ccgcgcgcag catgtcgaag 108840cggtggtagc tgtccaggcg cgcgcggcag ccgaagaagc gtgcgacgca cgcggtgacg 108900atgtcgttca cgtagagcac gcccgaggcc gtgcagtaca cggagcgcgg ctcgcgcggg 108960tccggcggca cgtccacggc gaccgcgtgc gcggccacgt cctcgagcac cttgcgctcg 109020agcacggcga ggaagtcgcg cagctggcgg cggttgtcca gccaggcgta ggtggtcgcg 109080aagagcgtga gccgcccgcg cggcgcgatc gcggtgtagg gcgcgtaccc gcggaactcc 109140cgggggtgca cgaccttgac gttctcgtgc tcgcgacgga aggcctcggt gtcgagcagc 109200gccgcgagcg cgtccacgag cttgtcggag acctccacgc ccgcgccgaa ggcgatgagc 109260tcgatcttct gctcgctctt ggggcggaag tcgtggaagg tgtgcagcag catctcgcgg 109320agctgcggcg gcttctcgac ggcctcgagc gcgtcgccgc ggacgaggaa gtagtcgagg 109380tcgtgcagcg agacgtgctg cccggcggcg ctctgcacaa acttgaggaa gacgcagagg 109440cccgcgcggc gctcgagcac gtcctcgacg tgcgcgtgga atacgtgccg cgagggcatg 109500gcctcgatcg cggagagcca ctcctcgttg acgcaggtgg tggtgttctc cagcaccacg 109560ccctgcgtga gcgcgggcca ctgcaggtgg aaggcgaact cgtgcttgat gagcgaggcc 109620acggccgggt ccaggtccac ggccagcgcg gcctcgccga cgaggggagc gtccgccatc 109680acgcggagga cgcctggccc atctcctttt tcgccttttt attcaggatc attattcttt 109740cgttgacgag gtccatgagc atcttgatgg cggcggccgc ggccgccgcg tcgccgccgc 109800acatctgcgc gatgcgcgtg agcatgtgca gcagcgcggc ctcgtttagg tcctcctcca 109860tttagaggcc gtgagggcgc gcgtcgtcgc gacgagggga cgcctcccgc ggcagcgtgg 109920tgcgcacggc gaaggcgagc agcgcgccgg cgcactgcgt gagcacacac tccgcgagcg 109980cgacgaggag ctcggagggc gcgagcacca tttagaggcg cgcgcgggtt taattgccgc 110040cgtcagagtc ggcatcatcg cccttgtcgc cgccgtcctt gcagtcgccc ttggtctcgg 110100cgtcgacgat gtcggcgagc cgcgtcttca tgtgcgagaa ctgcgcgagc aggatgccgg 110160ggtcgagaca gcgcttgacg acgctctcgt cggcgaagtc gtagcagatg cgctcctggt 110220tctggcagaa caccgagtct tcgatgatca acaccctcct ggtcccggcc gaccgcatga 110280tggccatggc ccggatgagc ctcttcttcg atccgcgtat ggacatggac cggagcacgt 110340tctccacgtc ggagtcggag acgttgcagc agcagaggtg cgtgatgctg gcgcgcccgt 110400tgacggggat gtgcttgtag gtctggcaga gcagcaccag cgacacgttg atgtgccgcc 110460cgtagttcat gaggcccaag agtgtgggcg accgcgtctg cgtgtcgccc atatcgtcga 110520gaatgatgag gaacttctgc ttcttcgtct gcgcgtgccg ctcgatcttg cgcttggcga 110580ccgagaggtt gtactcgagc tcctcgtgcg tggtgacctt gtggatgtgg tccggccaca 110640cgaagccgtc gtaggcggcg ttgtagacgg gcgtgaagag caggatgtgc ttgaagcggc 110700gcacgagcgt gcggaagagc gagagcaggt aggcggtctt gccggagccg gagccgccga 110760cgagcgccat cctgaagggc gcctcgatga gactctcccg cttgaagcgc acctcctgca 110820cgacatccat cgtatattta ctgtcactaa attaccggct ccgagaaata tagaaattag 110880agcctcctag agcacaccga ggcgcatcgg caagatggca cataacacgt tcgaaaacga 110940tagcgagtcg gcagctaaca accagtacgt ggcgtcagtc aagcgccaga aaatgattcg 111000gcgatacatt aagatgttct tccggttcgt tacggcgata gctatcattg tcctggctat 111060tctagttgtg attctgtcgc tatctctaga cgaatgtctg cacagagaac accctcatga 111120ctattcacat gtacaaaatt caacatgcga cggcattact ttaggtggtg aaaagtgtct 111180cagacttaat ttgccagcaa cgtgggaaga tgctaataga caatgtggta atcttgggtt 111240ttacctacca tctactggcc ttgaaaagaa atttccttgg cttgtgacct atctcgacgg 111300aacttgggga aacactcaga actccgtatt tggaccaacc ggtgacttgc agaatgtcat 111360aggaccgaaa gaatacaaat atttttgtgt gtccgattag atgattataa tctaataaat 111420gggttgctgt aaggtcccta accgccagtc tataaggact ttgaaaaagg cgtcctgtcc 111480ggtcgccagt ctcgtcacca ttctctccct agtcaccagc ctcggtgcga tagtcagata 111540caccaatttt tttctaaaag aagcatgtga cgaaggatgg atgcccataa aaaacatatg 111600cattttaaac acgcactttg aagccaccaa tgacgatgcc cacaggatat gtgaaaacct 111660agacggaaag ccgccggcca tccctaaccc tactctgtta aagggtgtca cagttctcac 111720cggcgaaaag aaattttgga tgacccatca cgaagactat actactgtgt ttgagcatat 111780agacgatagg acgactccta aaaacacaga ctatgacagt aaaaaacaca cttgtttgat 111840gagcgaggac ggattgatac accataactg catgatgaac gtgactgtgg tatgcatgaa 111900ggagatgcac ggataactga aaatatactg tttgaacgca aagacgccat gtcgcgactt 111960caaatactga cctcatttgg acaaatcttc gcacccgacg aagctcggct gcgcgagatc 112020gcgcgtgatt tgggaatatg caccataaaa cgcgcattcg gcgacatgct gtacggcttt 112080atagacttcg acccggtgcc cctgacccaa gtaaacatgc tcatgtccaa ctgctacttc 112140gcggtcaacg gcaacctgct tccgtgcacg gaggacttcc ggctcagact cccggcaacg 112200gagatctctg cggcctacct gacgagaacg ggacggacga tcctgtgcgg caaagacttc 112260aacatagtag cgccgtcggg gttcaagccg tccatgcggc tgcgcgacct cagtcacgtg 112320tctgcgcttg tagagatcct ggaagtctac gacgagtccg gggagtacca attcgtgctc 112380ggccccagcg cgcagttcat gctgcggctg atggagaagg agaacgtctg tctgttcggc 112440agcgggtggt gcatagtgga cctgcgcaag ctggacgtac ccatataatc agcatccttg 112500tttttatcct gtctttttat cagtttttta gctagttaaa acataaatag taaagctaaa 112560aagaggagtt ctggagtctt gcaacaacca ggatgaaggc ggtgttgttg ctggcgttac 112620tgggagcgtt caccaacgca gcgcctttgt tagaaagcca gcgttctaac agtgaggaaa 112680aagcaaattt ctgctcgacg cataatgatg aagtgtacgc caggttcagg cttcagatgc 112740gcgtgggtgt acgacacagt ccgctctaca ctcccagcaa catgtgcatg ctggacatag 112800aagactccgt tgaggacata gaagagtcca cagaaaaaga atacgcgtct acggccacgg 112860gtgaggcggc cggagtgaac gtgtcagtgg cactagtggg agaaggcgtg agcataccgt 112920ttagttacat aggccttgga ttcaacccgt cgcttgaaga tagctacctg tacgtcaacg 112980tctcgtcacg agctccttgg gttaaacaga cttcggacct atccgcgaac ggcggctggg 113040gtatcaaaca ggttctagaa aaagagttac tggccatcca aatagggtgc gacaaccaaa 113100aatttcccga agaacccaca actacacccc cctcacctgt cactacaacg ctttcctcaa 113160caactccaga tctgaatgaa gaaaacacag aaaatacgcc gacgaccacc ggcgccagtg 113220tagacagaaa gcgcaatcca gctgacattg acttctcgct gctcgtggac ccccgatgcg 113280tgacctctgt agacctgcac gtcgagctca gggacgcgtg catagactac aaacaagagt 113340cgccgttgtc gctgaagggg aaatatggag acggcgaact agtaaaaaag gagattaaag 113400acgtgggaaa gaatcacaat atgtgcagtc ttaacctcaa ccctggcaat tgagctgttt 113460ttattcggca atataatagg tgattattga acattaaaca aaacttatcc cacaacgccg 113520caacaatgga agtgctggtg atcatctcta ttatcgtcgc cgtaatatgc ttgaccggag 113580cggtaatgta cctccttatt gaactcggct tagccgccga gcgcgctaac aaacgcgcgc 113640gcgtgaagaa aaatatgcgc aaattagcca ctcaattggg aaatggatct gtcgactccg 113700gcataggcat aggcccgtgc ataatgtcgc gcaccatgga ctctggaccc agtcgctggg 113760acagcgacag tgaggatgac ggggacagcc tgtccacgac gtccaccagc ggagggggga 113820ctctcacccg agtgtgggtt gggagcgccg ggcctatgta cgaaaacttc tgcgggaacg 113880gcacccgcca ctcccccacc aacgaccctg gctaccactc gcgggagact ctctgcagcg 113940gacctccccg tcaggcgccg gcgctaccgc ccaccccgaa gcccgacgag gtaacggtgg 114000acgtggggcc cggtcccgac gaccaacacg gtccgtacga ggaacctgat cccattcccc 114060cgcaggaacc cgagccgccg gtgcagattg aggtaaccat caacgggccc ggtggagaag 114120gcgaggcgga aggagaattt ttctacgacg agtagccgcc aaaactgaat aactatcggg 114180cttcgtaaac gcgcagacat gccgctgttc cggaagctca tggtttcgcg ctccctggtc 114240aaggaatgtc tgactctgga cttccggcag ggcgagcgtc tccctacgcg atgcttcctc 114300ccggtgcccg cggggacgac attccacaga gtctgcgaca cctcgccgct gacggacgaa 114360gtctcccggc acgtgcagga gcccgtcatg ggcaccggac gggtccagta ctactacttc 114420gagagcggcc agggcatgat cggcgacaac gcgggcatgg cgcgcatgct cgtgtgcacg 114480cggtcggcgt acaacggcgg cgacgtcgtc gtgcggtcca cgcggagcag agcagacaag 114540accgtggtcg cgccctgcca gggcatggcg ctgctgctga gccccttctg cgccttcgac 114600atcacgccgg tcgagagcgg ctccgcgata ttcgcggagg tcatcgtcac cgcgcccagc 114660atggactacg tcgaggcggt caccggcacg ggcgaggcgg ccgtgcggat attcaactcg 114720caccacccgc tctggccgcg acacggctcg aacgtctgct tcgcgctgcg gttgctgcga 114780gacgtgcgca cgggcgagcg cgtggtcgag cagatgttca tggacgggcg ctggcacacc 114840gtgctgagga cgtcctgcgg caacaaggtc tgcgtgcccg ccgacctcgt gggccagacg 114900aacctcgagg aggtgccctt ctgcgacgtg acgcccgaga tcatgcgccg cgcgctggcg 114960atcgacccgc cgtacgaggc cgtggcgcac ccgcaccgct gcgtgtacgg cgccatggac 115020gtccggtgcg cgaacgagta cctcgtgtac tgcaccttca agacggagcc gacacggcgc 115080agcacgtcct cgccgggccc ggacagcccg ctgtcgcccg cgactccgtc gacctcgcgg 115140gccgcggccg cgcgcgtccc cacgacgccg caggaagtgg cctcgccgac cacgaggctc 115200ctggagacct gtctgcgcga cgccctcgac ggactctgac ccgaaggacc caccgtccac 115260tcacattcca ctgccagaca actcaagctt tttctgcatc tacctcgcta ataattgaat 115320tgttatagga caaacaggcg cactcgagca caatggcgtg ttttatcgaa ttgttagact 115380ccatcttcaa ccgacgccac cgtaatttcg ggccggagga catgtacagg ccctctgacg 115440ccccgccccc caaatcgcac acgcctcgca ctccccgcac cccgcggacc cagtgtcccg 115500gacacccgcg gcgacaaagc tcctctccca tctacggcgc ttatgtggac tccctgccga 115560ggaacagaaa gcggttccag aatcaacaca gttgtcccgg agattacgag cggtgtcaac 115620tctcggacac catcagcctg gatgcgacgc tactcacggt tacagtgacg tccatctcca 115680gcatatccag ctctagtagc tcagactcta tctctctggg gcagtgcaga ctgtccatgg 115740tgtccgcaac atcaacctcc acctccacaa ccttctcctc ctcggaatga gcgccacact 115800tatttttgta taatagtttg tattgaacct tagagacatc cacaaatagt taggaagcat 115860gagtagttca agtagcgaga ccacccctaa gcccaagccc atccctgctc ctcccatgac 115920tcaggaggag tttaacaaag aagtgaagaa acgaaaagaa cagaaaaagg aaaaatctag 115980aaccgttgaa cgtgagtcag aaaccgtaac tgtatcttcc gacggatcag agataaaaaa 116040gacttacgag cgcgagtctg agagaacaac cgaaacagaa aagaacaaca cgtcaaccga 116100tgataataag cagaacaccc ctgtagagaa accagaggaa accaagcctg cttctactcc 116160tgaaggtgtg aagccagccg agactcctgc cccgactact gacccccaac ctactacaca 116220accacccgca gaatcaaacc ctggaagtca acccgcacct gcttcagaac caacccccgc 116280acctgagcct gcaccggaac ccactcagcc tgcatcagta actcaacccg ctccaacacc 116340agagccaagt ccagccccta agcctactcc ggcttctgaa ccaaccccag catctgagcc 116400tacttctgct ccagaaccta caccatccgc agaaccaact cctcaaccaa ctgtagaaac 116460accaccatct gctccagcac caactcccga ggcccaacca cccgccaaca gcaatcccac 116520tactgaaact accactggta ccagcacctc ctaagtgagt acgtaagcat ttcggagtaa 116580cgtcgtagca agcgctagtc cgccgcgagc ggttctcgca agttttttcg ggtaaaaagc 116640gtacaccgtc gccttgtcgc ggcggtgtac gcttttttca cgcccttttt gcaaaattta 116700aattgtaccc gcgccggctc taggaaagat ggcgtgcctc agagtgttcc tggcggtgct 116760cgcgctgtgc gggagcgtgc actcggcgca atggatcggc gagcgcgact tctgcacggc 116820ccacgcgcag gacgtcttcg cgcggctgca ggtgtggatg cgcattgacc ggaacgtgac 116880cgccgcggac aacagctcgg cctgcgcgct ggcgatagag acgccgccga gcaacttcga 116940cgcggacgtc tacgtcgccg cggccggcat aaacgtcagc gtgtccgcga tcaactgcgg 117000cttcttcaac atgcgccagg tagagacgac gtacaacacg gcacgccggc agatgtacgt 117060gtacatggac tcctgggacc cttgggtgat cgacgacccc cagccgctct tcagccagga 117120gtacgaaaac gaaacgcttc cgtacctgct ggaggttctg gagctagcga ggctgtacat 117180tcgcgtgggc tgcacggtgc ccggagagca gcccttcgag gtgatcccgg ggatcgacta 117240cccgcacacc ggcatggagt ttctccagca cgttctacgg ccgaaccgcc ggttcgctcc 117300ggcgaagctg cacatggacc tcgaggtgga ccaccggtgc gtgagcgccg tccacgtgaa 117360ggcgttcctg caggacgcct gtagcgcccg caaggcgcgg acgccactct acttcgcggg 117420gcatggctgc aaccatccag atcgccggcc aaaaaaccca gtaccgcgcc ctcagcacgt 117480atcgtcgccg atctccagga agtgcagcat gcagacggcg cgctaagggc gctcaccgcg 117540ctgacggcgg ccgtggtgtg cgcgatcgcc atcgcgctcg agcgcggggc ggaggccgac 117600gccgtggacc ttatccttat aaaattttca atgatatgct agtttttatg cgaccttcct 117660tagaaaattc ggaattcaaa aatgaaataa aacggcgttt agcacgcata ttattaatac 117720cgaccaccat ggcaggcgtc cgcagctgcc agaagaaagt cccttctact gcgggctcca 117780tgtcatttca acggggcaac cggagcatcc agccggcgat gtccgaggcg ttgcagaatg 117840atttcagcta caacccgcga ccgcctccgc cgagcgcaga agagattgac ttcttctgcg 117900tggacatgcg caaagtactg atggaaattg aggccaagcc cagcagctcc aagtaccccg 117960atttcatcca cccggttgac agcagcccgc cgtgcacgcc ggcgcgcaag cgcaacggct 118020tcggccgcaa ggcactgaac aaaacccagg tgccgcagca ggccaagcgt gacggctact 118080cccgctaatg cagtccacac acttcacaca ctacatcagc actcaagctt ataatcacta 118140cacaatgaat cagcccacca cgtgcgaagc acacacataa aatcacccac ctgtcctgat 118200cgttcccaat actcccaatc accgtgcttt acacgcacgt taatcaccct ttccttcctt 118260catgcgttcc tgatcgttcc tcctccttaa tcacacacac accccgtaat tttgtacttt 118320tgtactttaa tttgtacact ttacacactg actttgtact gcctttgtac tttatttttg 118380tactgaaatt ggacgatact tatctttgta ttcacatcca agttttgcaa attccacagc 118440cggtagcgaa aagtgaaatc gtaccgtttt aggcttcgat ccccctcccg cgcgaagact 118500cgccagcatg gactctcgta ggctcgctct tgccgtcgcc ttcggaggcg tcctcgccag 118560catgacacag cgccgccgcc tggcttctct catcgccagc atcggccaac ggctgatggg 118620cggcgacggc atgcgtcgcg tcgccgttcg gttgatcgac cagctcatgg ccggaccccc 118680ggacatcaac gacgaggcct tccagcgcga gatccgcgtg ggcgagctct tccaggcgct 118740ccaccgcgtg gtcgagcagg cacgccgaga gaagtacttc gaggtctgcg gcgccggcaa 118800cgacgccgac gcgcccgtcg tcgagatgga caccgcggcc gcacccccgc agccccagcc 118860cgcgcccctc gtggtcacgc cgcagaacgc gttcatgttc gtgccgcaag gcagccacgt 118920gcacgtggac gagagcgtgg acccgttctt cggcatgagc ccctccatct tcgggcgcga 118980cctcccccct cagccgcccg aggagctgct gagcgactac gacccgctca tgagccaggc 119040cggcgagccg ccgagcccgc ggtcgccctg cgaggccgac ctctggtgct tcgagacgct 119100cggcgacagc gacagcgatt gagaccgcac cacaccctac ctcacccacc ccacactcca 119160cctcacctca ccctaacacc cgacacccaa cacttcaacc ggacaatgaa ggagtcccac 119220atttcactga aggacgcgga tgaagccgca ctcccccaca tgaaggattg gcaacggtca 119280aacatttcac ctgcaatgaa ggacgatgcg cggtcgcatt ggcctgcgac cgacatcgca 119340cacatgaagg acacaattgg tttgttaatc cggacaatga aggacaaatt gtttttgtta 119400atcaggacaa ttagaacaca atcaaatttt tgtacgatca taaaatcgat atttgatgca 119460catatattag taagtatatt agactaaatt ctccggggag gcaagcagtt ggatacggcg 119520gggcggggca cgacgtgcac ggagagttcg ggcgggtccc ccttcccccc acccccacgg 119580caccacgatg cgcctaatct tagcgctcgt ggcctgcttg ttggcggcgc cgatgccgtt 119640atcgggtcgt tcgacaagca ctccaaacac gtccccctcc gcactcggct cgacgagttc 119700ggaaccaagc tcggaagacg ctgtggcttc gagcacaacg acaagcacac tcacaagcac 119760tacaagcaca ctcactatgt ccacaagtgt ggacaccact actacctcgg gcgctacaac 119820gtccgcaaac agcactcctg cagcgagtgt gagctcctcc acacccgcaa ctaccgaggc 119880atcgacggca ccaacgacgc cgtcgacgcc gacgacagtg aaggtaacga agggcaagga 119940agacacgaag gcgtctgcct acctcgtttt actaatcacg ttcatggtca tgaccacgct 120000cgtgatggtc gtggtcgtgg tcgtggtcgt gtacaaacag ggactctgta actgctgctg 120060taagtttccc ctgctgcaaa gagctcaagg actacctcga cgaggaggag agcgccgggc 120120tgtacgacgc cttgacgtgg agccactcag actccggcct ccggctcgtc gtgcgcgcgg 120180accccagatg atgaggatcg gataagatcg gcgtgttttt cccgcccgtc gcgaacatta 120240tgcctctaaa tgccgagaat taactgaaat tcaaacacgc tttgggactc aactccgtga 120300cccacactca accatggctg gcttcctagg cgcattcaga ggcgtgtgct ccgacttatg 120360gcagtcgctc cgtggacacg gacaccactc ttccagctgc ccgcgacgac gcgccaacag

120420catggacgac cgcgaccggc gccgacaccg ccaccgcgag atccccaaca gctcggcgtc 120480gctgaacagc gacccgatgc cgcaacgcag tgcgggtgcg cgccggcact acgactgccg 120540cccctcggaa aagagcagac actcctccga caagcaccac tcggcggacc gacaccactc 120600ggcggaccga caccaatcgg cggacaggga cagacaccgt cgcagtcgca agaactacga 120660ctcgcacccg tcgcgcagga accgcaacta cgagcgggcg gactaccaga gacacccctc 120720acaaacccac ccagacgccc ccgcgcagac ctcgacgctc aaggtgacct ccctaagcac 120780cagctgcagc accctgtccc aacatcacta cgagaccccc gaccacatct acgacatccc 120840ggaagacagt cgcggggcgt cggctccccc tcgcgcggac ctcgcgctcc ccccgctcgc 120900catgcccaaa tccaagccgc gtcgcacgcg cccggcgtcc atgaacgact gcctgatgaa 120960gcactgcggc gccggcagac ccaacctcca agacgacata tgcacactat gtactgatat 121020agagacacag ctgagcgcac tagagaagtc tctggagtca gagctcaact tctatcgtcg 121080ctacatacaa gacactaaga cattgctcgc cacgcgagca gcaaacatcg gcagcaaagc 121140tctgatctac accgacgact acaacggcag tggcgacgtc ggcgaaaagg agcactgctc 121200ggaggagtgc tgcaaagtgg aggaagttct gtgagaaagt gcgtttttct gtaatgtgaa 121260ataagatagc cttatgtgtg cacagacatg gcgaacaggc tcgtgttttt cgaccccgag 121320accctagccg aggccgacgg catccccggc tatggggtgt tcgagcccgg caagaagaaa 121380tgcatcttca caaagatccg caccagcgtc gcactcgcgt gccggtacgc cgtctcggac 121440ggcggcctca tcgacgagtt cgtcatggct acatacggga ccagacgcgc gtgccggctc 121500gtccggcacc tgacgatagg cgcggagggc gtgatgaccc ggcccgccag caactgcgcg 121560ccgcacatgg tgctcatctg cctcagaggc gtggccgccg tgtccagcga ggacatgggc 121620ttcggtcgct gcatcatgga gcgcggcacc atgttcatgg tcaagtccgc gcacagcgcc 121680gtcgtctgcg gcaaccccgc ctgcgagctg ctcgtcctct tctacgacta cttcaccccc 121740atcccccggc cgctctccgg agacgaggtg ctgttcaccc gcgacctcgc gcacgtggac 121800tacgcccccg agtcggcggt cgtcttcaag atggattaca acctcgagac cgacgtggcc 121860acgctgtttg tcggggggta catattccgc gccaagggcc tgatgatgga gacgcgcgaa 121920caagtgggcg acgagtgcga ctgctgccgc cacagctcgc cggtgctcgt catggatcgc 121980gagaagatga tgtcgtcgct gcgcatgatc cccagcatcg tgcccggcca gcgggagatt 122040tggcttcgcg agcgcggctg ggccgtcctc gagacggacg cccgcggaca ctgcgagccc 122100ggcgtcctga ggctggcgct cgccggcctg cggctgttcg caggatgcct gcgctccgtc 122160gtggggcggc gcgagctgtc gctgttctgc tacggcgtcg ctcccaagtt cggcggggag 122220ttcgaggacg cgccgcgccc catggagatc gacggttagt tgtttttatc cctgtacata 122280cgccgcaaac tgaaacttta gggcaccgcg taatagtgca cgaacgccca gtggaccgct 122340tccgcagcca tggaaaacaa cgacggcaac gaacgcaaca acgaacaccc gcacgttcga 122400gaattcaagg aggcgtccct gtacgggttt ctggtggcgg ccgcggacgt gaccgtcgaa 122460gacgtgcacc ggtaccttca gttcggcgcg gacgtgaact acaggggcgc gtacctgtgc 122520acgccgctgc acgcgtacct gcagtccggc tgcgaaaagc gcctagacgt cgtggacgcg 122580ctgctggacg ccggcgcaga catcaacgcc aaggagatct gcggtctcac gcccgtgcac 122640ctgtacgcga gctacgcgga cgtggacgta gagttcatgc gcgggctcat cgagcgcggc 122700gcgagcgtgt gcggcgagag ctcggtcacg ggctgcctgt actcgtacct gtacacacac 122760agcgtggacg gcggcgcgcg cctggacgtg gtcgagctgc tcgtgcaggc gggcgcggac 122820gtgaacgtcc gcggcgaggc gcgcaagacg ccgctgcacg tgcactgcgc gggcttcgag 122880gtggattcgg acatcgtgga tctgctgctg cgcgcgggcg cggaccccga ggcgctcgac 122940gaacacgggc tcacgcccgc ggacgtgctc gtgaagtccg tgggcgccaa cgtggagacg 123000ctgcggctct tcctcgacgc gggcgtgagc gtggccacgt cgcgcgacgc gcgcggacgc 123060acgccgctgc accaccacgc agactccttc cgggcgagtg cgggcatcgt gcgcgaactg 123120ctcgccgccg gctgcgacgc ggcggccgcc gacgacctcg gaaacacgcc cctgcacagc 123180ctcgccacct tctgctcgtg ccggcgctcg gtgctcgacc agctcatcgc cggcggcgcg 123240gacatcaacg cccgcaacca ctacggccac acctgtctgt actacgcgtc catctacaac 123300ccctccgtct gctcgcggct catcgccgcg ggtgcggacg tgaccgcgcg cacgccggac 123360ggacgcacgc cgctctcggg catgatcatg cgcaagcaca cgcgcgccgt gcgcgccgcc 123420ctggcgacgc ggcctcccgc ggacgccgtc gccgcgtcgc tggacgtcgc agtacagccc 123480gagcccacgg acgccactcg cgcgtgcgtg cggtacgtgg tgctctgcgg cggcacgctc 123540tcggcgcgcg tgcggtcgcg acacgcggac ttcgtgcgag agtgcgaaag cgaggtggtc 123600gtgctcagaa ccaccgtggt ggggctgccc ggcacctcgc tgctggacat cgtgcgtgcg 123660gcgcagccgc cgccggtact gctctccccg cgcgtgcacc acgtgctgca gaagctgtgc 123720gtgtacgcgg agttggtgga cgcgcggctg cgcgagatgc ggcacaagac caacctcgtg 123780gacgcggtgt cgcggctcgt gtgtccgtgc gcgctgccgc cggaggtggt gcgcggcatc 123840ctcgtgcacg tgccgataga cagcctgcgg cacacgttga ccctcggcgt ggcgcaggcc 123900tcgcgtttcc ttccctcgca taaatgaaat attatttttt gtggtagacc ggatctcccc 123960gatggacccc gccggacaac gactgcgcgc gccagggccg tggcgcctga acccgccgac 124020cgcggccgcg ctggaaagcg cgctgctgcg gcccgcggcg tcggcgggcg ccgaccgctg 124080cgcgaacgcg cacgtggacc gccgcaacat gggcgtcggc gagggccgcg aggtgcccgc 124140ggacgtcgag gggctcatga ccgagatcca cctgcggtac ggaatgacgc gcgtccaccg 124200gaacgttcac ttcgtgcagt tctggcacgg cgagcacgtg cgccggcgcc ccgcgcgaca 124260cgtgttcacg gtctggatct gcctcagcgg cgaggtgcgc atctacgcag agtgctgcca 124320ggcggggcac ggcttcgtgc tctgccgaca gatggcggca gggtacatgt tcgtgaccga 124380acccacggac tcggtcacgg tctcggtgcc gcaccgactg cgcaactcgc ggtcgccggt 124440gtggctggcg gcggtcttcg ccacgcggca cttcgagccg ctgccgccgc ccatgtacgc 124500cgtgcccggg cacgtggtgc tcgcgcgcag cgcctccatg ctctgcgact gctggccgtc 124560ggacccgcgg cgccgcaacg tgatcttcta catgcggctg tcgggcgcga tggtgcgcgt 124620ggtcgtgccg ggcgcggagc tcgagatcga gtgcacctcg gggttccggc cggaccactt 124680ctccatcaac gacgagtgcg tgtgctgcga gcgtccgcac gtcgcgcgaa ccgcggtgtg 124740gacgctggcg gagatttgcc gcggcgccac ggtggtgctc gcgccgccac tgccccgcga 124800ccgcgccgcg gggctgctcg cggagatccg cctggcctcg ctgcgatggg tgcgcgtgcg 124860tgcggtccgc agcggcagag aaagcgtggg cccgttcccc tcggtggtgt gggcggcggt 124920cttctccgcc gttcggctct tcctggacgg aaccgtgcct gccttcccgg cgtgtgtgga 124980gaatggacgc gcggcgtacg gcatggtgta cgtgcccccg gaggagccgc ggatggacgg 125040gctctgtgtg ttcccgacgc ccgccgagcc ggcggcgctc ttcgtccgcg gagaccaggt 125100gcttgaggcc ggcgcggccg ccgccataat cgcggccgct gagaagcgcg tccaggccgc 125160caatgggtct cctgctgccg cggaggagga cataggtgcg gcggccgatg ccgccgcaga 125220gagcgtggag caggaccagc gcgtcgagtt cgaccttggg cctgggcctg accccagcca 125280agaagcgccc gcggacgcgc agcgtgccga ttcggacgac gacaccggct ccgagaccga 125340gaccggcgac gagagtgtgg gcggcgagga tgacagcgac tcctcctcct cttactcggt 125400gatgtcggac gacgaaaacg acagcggcga cgagggctgg ggcgactcta gcgactccgg 125460catcgaggac gacgacggcg gtgtcgccag gccgccgagg aagaagagga ggaagagcgc 125520gacgtcctcg gcgcagcggc ccagatgctc ggagactgac cggtggtgaa aacataaaaa 125580taaactgttc aacacttgta ctccgggcac caacactact atccataccc accctccctc 125640cacacactac aatggcaaac agagaagaga ttgacgcctc cgccgtcatg gctgcctacc 125700tcgcgagaga gtacgcggcg gctgtagaag aacagctgac gccgcgcgag cgcgatgcgc 125760tcgaagccct tcgcgtttcc ggcgaggagg tccggtcgcc gctgctgcaa gaactctcga 125820acgcgggcga gcaccgcgcc aaccccgaaa actcgcacat ccccgccgcc ctcgtctccg 125880cgcttctcga agcccccacc tcccccggcc gcatggtcac tgcgattgag ctctgcgcgc 125940agatgggccg ggtatggacg cgcggccgcc agctcgtcga attcatgcgg gtcgtgtacg 126000tgctcctaga ccgtctgccg cccacggccg acgaggacct cagcacctgg ctgcaggccg 126060tcgcgcgcgt gcacggcacg cggcgccgcc tgcaccgcgt tctcggcgtc ggggccgtca 126120tggcaggcgt cggtatgctg ctgctcggcg tgcgcgtgtt gcggcgcaca taacttttta 126180tctcggctca aactgaaata cgacattgga ctacgaaacc tatgattttg ctcacggccg 126240cgcgagatag gataataaat aacctttgag caactaacat ggccgatgag agagaggccg 126300acggcgcgct gttccggtac ctggagagcg aggaccgtcc ggacgtggag cacatgcgcc 126360ggctgctgga cgagggtgcg gacgtgaact acgcgggccc gcgcgggtac gcgccgctgc 126420acatgctcat gcgcggcaac ccgctagacc ccgacgcggt gcgactgctg ctcgccgcgg 126480gcgcggacgt gaacgcgaca tcgctctgcg ggttcacgcc gctgcactcc tacatgtgct 126540tcgggaccgt gacgccagac acgctgcgtg cgctcatgcg ccacggcgcg agcgtcagcg 126600acctcgagcg caacatcaac gcgctgatcg agtacttcaa ccgcgacggc tgcatgggcg 126660gcgcggaggc gaccgtgatc gcactgctgg tggagcacgg cgcgcacgtg aacgccaaag 126720acgaccttgg acgaacgccg ctgcacatct acctgtccgg cttcttcgtg tcggcaccgg 126780tggcgctcgc gctgatcgcg ctcggcgcga acccgaacgc cacggacgcg tacgggcgca 126840cgccactgca cgccttcctg cgctcccgcg acgtggaccc cgctgtgctg aagacgctca 126900tcgccgcggg cgcagacccg ctcgcgcgcg acatcatccg gcgcacggcg ctgcactacc 126960actgcgagtc cttcaagacg cgcgctagtg ttatagagac gctggtggcc gccggctgcg 127020accccgcgag cacagacctg ctcgacaaca cggcgctgca cagcatggcc atgggcagct 127080cctgccgcgc ctcgctgatc cgcccgctgc tggccgcggg cgtgtccgtg aacgcgcgca 127140acgcgcggct gcagacgccg ctgcacctcg cggccgtgtt caacccgccg gcctgcgcgc 127200ggctgctggc cgcgggcgcg gaccccgcgc tcgcggacct ggacgagaca acgccgctgc 127260tgagcatggt gcggcacaac tgcgcacgcg cgctgcgcac ggcgctgccc ttggcgccgg 127320acgcgctggt ggccggcgcg gtgaaccgcg tgaacgcgcg cacgccgagc gcggccacgc 127380gagagtgcgt gatggcgctg gcgctgcgcg gcgcgctgga cctgctgagc gcggagagcg 127440ttgccaccca cgcggccgcg atccgcgcct gcgaggcgga ggtcgcgctg ctgcggagca 127500cgcgcctggg cgcgccgccg acgacgctct tcgcgctgct gacaggacga ccgaacacgc 127560tggtttccgc gaaggcggcg cgacgcgcga tggcggacgt gtgtgtctac cgcgcggcgc 127620tggccgcgcg cgtggagcgc gtgcgccgca agtcctcgct ggtcgagcgc ctcaccgcca 127680tggtgtgtcc gtgcgctctg ccgccagagc tagtgacgcg catcctcgcg ctcctgaccg 127740tggaggaact cgcttgcgca atgcgcaaat aataatgaac tataactagg cttattagag 127800gcactatttg tgcagagtcg ttagttatag ttagtgtact taccattgga atgtcgaaga 127860acaaaattct ggtgtgtttg gtaattattc ttacttatac attatacaca gatgcgtatt 127920gtgttgagta tgaggaaagt gaggaagata aacaacagtg cggtagtagt agtaattttc 127980ctgcgagttt accgcacatg cttagagaac tcagggcagc gttcggaaag gtaaaaactt 128040tcttccagat gaaagaccaa ctgaacagta tgctactcac acagtcgctc ctcgacgact 128100tcaaaggcta cctcgggtgt caggcacttt ctgagatgat acagttttac ttggaagagg 128160tgatgccgca ggcggaaaat cacgggccgg acatcaaaga gcacgttaac tcgctgggag 128220aaaaactcaa aacgctgcgt cttcgactgc gtcgctgcca ccgcttcctg ccgtgtgaga 128280acaagagtaa ggccgtggag caagtcaaac gtgtgttcaa catgctgcag gaacgaggtg 128340tttacaaggc catgagcgag ttcgacatat tcatcaacta catagaatca tacatgacta 128400ctaaaatgta aaaatgtata caacttttag ttatcgttcg gattctcgta tcgttctgca 128460tactatgtat ataaaatgta tattaacata gttacagtta cagttacagc tatattttta 128520tgctcacaag atgctatata attgaaagga aattgttcac tctctgtcag ggcgccatgg 128580actttctagg cgccgcgctt cacgactacg ttgccgatgc gcccaaggtc tgcgccgagg 128640aggtgcggcg gctgctggcc gcaggcgcct ctgtggagta cgcgggcgag ttcgggaaga 128700ccgcgctgca ccagtacatg ggccgttccg gcgcggaccc cgccgtcgtg cgcgcgctgc 128760tggacgccgg cgcgcgcgtg gacctcccgg agacctgctg cggctgcacg cccgtgcacc 128820tctgcctcat ggccgccaat atcgacgtgg aggttctccg catgctcgtc cacgagggcc 128880gcgtcgagga ctgcggccgc gccgagctcg cctccgtggt gctcaaggag ttcgtggtga 128940accgcgcctt cgacgagaac gtcagcgagc gagtgatgcg cgttcttgtg gccgcgggcg 129000cggacgtgaa cgccgccagc gtggtcgacc gcacgccgct gcacgtctgc ctcacgggca 129060tgtccacgca cccgggcacc atcgccgcgc tgctgcgctt cggcgcggac gtgaacgccg 129120tggacctctg cggcatgtcg ccgctggcgg tgctcgtgcg ctcgcgcgcg gcgaccgcag 129180agctggtgcg catgctgctc gacgcgggcg cagacgcaca cgctgtcgac agtcgcctgg 129240actcgctgct gcaccagcac tttcagtccg cgcgcccgcg gccggaggtg gtgcgcgagc 129300tcatccgcca cggctgctcg ccgcgggcgc ggaaccgaat cggcaacacg ccgctgcacg 129360aggccgcaaa acactcctcc tgcaaacact cgctggtggg gccgctgctg gctgccggcg 129420cgagcgtgga cgcgcgaaat aacacgggca ggacgccgct ccacttggcg gtggcgtcca 129480acccgcgcgc gtgccgccgg ctgatcgcgc ttggggcgga cgtggtcgcg cgcagttacg 129540cgggcgtcac gccgctggcg cagctgatcg cggacaataa ctccgcgctg gtgaccgcgg 129600cgctgaacac gcagcccgag ccgcgggccg tggcagagtc gctgcgagcg accacgcccg 129660tcggcgagac agcgtgctcg cggctctgtg tggcgtacgt ggtggcgcgc gcgccgagcg 129720aggtcctcgg cgagcccgag cgcgccctgc acgcggcctt cgtggcggag tgcttagcgg 129780aggtagcggc catacacgcc gtgcgctgcg gcacacctcc ggtctcgctg ctggagatcc 129840tggtggccgc gcgcccgccg cggagcctgc tctcgcgccg cgcgtggcgg ctggccagcc 129900ggacgacagt ttaccgcgcg ccgctccgtg cacgcatcgc ggccatgcgc catcgctcgc 129960gactggtgga gcgcgcgctg cgcacgctgc gcggctgcgt gctcccgcgc gaggtgctgg 130020agcgcgtgct gcggtgtctg tccacacagg acctgcgggc ctccggactg gccgagtagc 130080tttttctgag ataagtgaat aaacatggtg ggattcgatc ggcgccgcca acgccacgcc 130140atggacgccg ccgagatgga ggatctcgac atcaacgcgg agtcggcgct gtacgactac 130200ttcatcctga acgcggacag agcccgcgtg ggcgaggtgg ttatgcttct cgcacagggc 130260gcggaaataa actacgcgga cagcttcgac aagacgccgc tgcacctgta cttgcacacg 130320cgacacccgc gctcggacgt gattctggcg ctgatggagg cgggcgcggt cgtggacacg 130380ccggagcgct gctgcggcgc gaccgcggcg cacctgtaca tcctcaacgc ggccaaggtc 130440gacctgtcgg tgctggaggc catgctgacc tggggcgtgc gccagaacga ccagcactcg 130500gagcgggtgc tctcgagctt gttgcgcgag tacgtggtga cccgcgccta ctcggatcag 130560accgagccga tcatggactt gctcatcggc atgggcgccg acgtggacat gccggtcggc 130620gtgagtcgca cggggctgca cgcctgcctt acgggcctga acgcgaaccc gtgcatgatt 130680cgcgcgctgc ttcggcgcgg cgccagcgtg accgcaaaag acacctacga gatgacgccg 130740ctggcggtgc tgctgaagtc cgcgagcgcg acgccggagc tcgtgcgcat cctcgtggag 130800gcaggctccg acgtgagcgc caccgacttc cgcctcaacg gcatgctgca ccagcacgcg 130860cagtccacgc gcccgcgcgc gagcgtcatg cgcgagctca tccggctggg gtgcagccca 130920gcggccaaaa acatgtttgg gaacacgccg atgcacatgc tggccatgga aagctcctgc 130980cgccgctcac tgatcctccc gctgctggag gcagggcttt ccgtgaacga ggagaacccg 131040cactacggca ccgtgcctct gcacgtggcc tcggggtacg acaacacgca gggctgcctc 131100aagctcctcc gggatggagg agaccccacc gtcgtgtcgg ccgccggacg cacgccgctc 131160tcgaacatgc tcgtcaaacg caaccacgtg gcggtcgccg gcgcgctgtc gacgcacccg 131220agcgcggcag tagtcgtgca ggctctcgag caggctctcg agaacgtgct gaacgccggg 131280cccagcgagg cctcgcggct cgccgtggcc tttgtggtgg cgcgcgccgg cgcatccgcg 131340ctaccggagg ccgtgcgccg tctgcacgag ggctttgtcg ccgactgcga gcgcgaagtc 131400gagctgcttt cccgcaccat gctcggcaca ccggccgtga gcgcgctggt cgtgctggtc 131460agcaaggagg tctttggcac tgttatctcc tcgcgtgcgc tgcgcgtcgt gcgggaggtc 131520cgcgtgtacg caaggccgct ccgcgaggcg ctcataaatc tgcgccacaa atgccgctta 131580gtttccagcc ttaaaaggca ggtgggacct tgctcgctgc ccggcgaact ggtggagcgc 131640gtgctcgcga ccgtgccact gaccgacttg cgccgctcgt gcggccgccg cgcgcccgag 131700taactgcccg tcccgttgct acgcgactcg agactgcccg ctgtttttct ttccccgttt 131760cttcttatta ggagttgttg cccgcctcca tgatcctcgc acgcgccggc gggcaacctc 131820gcacgcccgc ggcggccgcg ggcgccgccg aggacggcga gcacagtgat cgccggaagc 131880gcaagcgcaa gacgcccaac tgcgaagacg ccgacaactc cgacgacgag ctagcgcaga 131940cgccgtgcga ccgcgagtgg ccggactgtc gcgcgagctc gatcacgagc tccgactcgg 132000tctctctcgg cgacgagatc tacctgcgat acgtggcctc gcaggtggac ttcgcgcaga 132060cctgggcccc gccagtgcgg ctgctgcgct ccttcgggaa cttctcgaag gaaacgctca 132120accgcatgtc gcggcgcggg tacgtgaacc gctcctactt ccagatggcg cacgcgcgct 132180tctcgcccac caacgacgac atgtaccaca tggccacggg cgggtacggc atcgtgttcc 132240gcttcgaccg ctacgtggtt aagtacgtct tcgagcaccg caacggcatg tccgagatgg 132300acgcctctac ggagtacacg gtgccgcggt tcctgcgcaa taacctcaag ggcgacgagc 132360gcgagttcgt ggtctgcgcg ctgcccatgg ggctgaacta ccggctgggc ttcctgcact 132420cgctgtaccg gcgcgtgctg cacacgctgc tgctgctcat gcgcgtggag gaaggccagc 132480ggccctcggt ggagatgtcc aagaagccgc tgctgcgctg gttcgaggcg cgcaaggaca 132540gcgagtcctt cgtgcgcctg atctcgtact tctacccctc ggccgtgcag agcaacgtga 132600acctgatcaa caacttccac cacctggtgc acttcttcga gcacgagaag cgcgcgcggt 132660acgtgttcga ccgcggggcc gtgatcgtgt tccctctggc gcgcgggtcc gcggactcga 132720tctcgccgga ggcggcggcg gcgctgggct tcgcgccgca ctcggagttc ctcaagttcg 132780tgttcctgca gatcgcgctg ctgtacctga agatctacga gctcccgggc tgcacgaact 132840tcctgcacgt ggacctgaag cccgacaacg tgctcatctt cgacagtgcg cgcgcgctca 132900gcgtgaccgc ggccggcgcg actttccgct tcgaggagcc cgtgcgcgcg gcgctgaacg 132960acttcgactt cgcgcgcgtg gccaccatcg agaaccgcaa gatcgcgggc agcgtccgcg 133020tgccgcagaa ctggtactac gacttccact tcttcgcgca cacgctgctg cgcgcgtacc 133080cgcacatcgc cgcggaggac ccgggcttcc acgcgctgct ctcggagctc acggtctcgt 133140gctcgcgcgg gacctgcgac cgcttccggc tgcgcgtgtc ctcgccgcac cccatcgagc 133200acctcgcgcg gctggtgcgc cgcgacgtct tctcccgctg gataaatgcc gccgcggacg 133260cccccgacgc cgccgcactc tcctgagccc acgcccgcgg cgccgggctc gctgtacgac 133320gtcttcctcg cgcgcttcct gcgccggctg gccgcgcgcg cggcgccggc ctcggccgcc 133380tgcgccgtgc gcgtgggtgc ggtgcgcggc cgcctgcgga actgcgagct agtggtgctg 133440aaccgctgcc acgcggacgc ggccggcgcg ctcgcgctag cctccgcggc gctcgccgag 133500acgctggcgg agctcccgcg cgcggacaag ctcgccgtcg cgctcgagct gggcgtggac 133560cccgagcacc cggagctgac gccggacccc gcctgcgcag gcgagagcgc actcgcacag 133620aacatcgaca tccagacgct ggacctgggc gactgcggag accccaaagg ccgccgactg 133680cgcgtggcgc tggtgaacag cggccacgcg gccgcgaact gcgcgctcgc gcgcgtggcg 133740accgcgctga cgcgccgcgt gcccgcgagc cggcacggcc tcgcggaggg cggcacgccg 133800ccgtggacgc tgctgctggc ggtggccgcg gtgacggtgc tcggcgtggt ggcggtttca 133860ctgctgcggc gcgcgctgcg ggtacgctac cgcttcgcgc ggccggccgc gctgcgcgcg 133920tagccgcgca aaatgtaaat tataacgccc aacttttaag ggtgaggcgc catgaagttg 133980ctcgtcggca tactagtagc cgtgtgcttg caccagtatc tgctgaacgc ggacagcaac 134040acgaaaggat ggtccgaagt gctgaaaggc agcgagtgca agcctaggcc gattgttgtt 134100cctgtaagcg agacgcaccc agagctgact tctcagcggt tcaacccgcc gtgtgtcacg 134160ttgatgcgat gcggcgggtg ctgcaacgac gagagcttgg aatgcgtccc cacggaagaa 134220gtaaacgtga cgatggaact cctgggggcg tcgggctccg gtagtaacgg gatgcaacgt 134280ctgagcttcg tagagcataa gaaatgcgat tgtagaccac gattcacaac cacgccaccg 134340acgaccacaa ggccgcccag aagacgccgc tagaactttt tatggaccgc agatccaaac 134400gatgatgcga tcaggtcatg cggaagaagg cgccacggag caaagtgaaa aaggaccgcc 134460tagcagtcga gaccctcccg ccgcagccgc ggacacccca cacccgcctt ccacccgcca 134520gacgccaaca ccgcagccaa caagcatgca cccctcgccg cgcaggctgc tcggcgcgct 134580cgcgctggtg gcgctgggct tcctcctcgg cgggctcttc cgccccgcgg cgccgccgct 134640gccggccgcc ctcgtggagg cgggccccgt ccgcgcgaac ggctccgcct cggtgacctg 134700cctgaccgtc ggcggcgacg ggcggcacat ggcggtggtc gcgcacggcg gcgggacgct 134760ctcgccggtg tacccgctcg ccgccggcat gcacgcgacc ttcgcctcgc tgcgcaaggg 134820cgcgctgctg ctgaacgtcg cgaccgtgca catctacgac gtgcgcgcgc tcgggccgga 134880gttcgagctg acctgcgtcg cggtggcggg cggctacaac gcggcctggg cggccgcgcg 134940gcccgcggcc gagtggcgcc gccagctggc gcggatgcac cgctcggagc tgtgaccctc 135000tccctcccgg tctcccatcc gtttttgtaa tcggccttag tagattagac cagcatcccg 135060cgcccttgtc cgagaacaag taacagtaac cgttacctca ctcgccactc ctcggaataa 135120tagaacgaga gaacgagaga acgagttaac cgttgctcac tcgctcactc ggtgtgagag 135180aacaagtaac gttgctcact cgctcactcg gtgtgagaga acaagagaac gagtagctgt 135240tgctaactca atcacccctc ggagtaagag aacaagagca gtcaactacc cactcagtct 135300tggatgagag gcagaggacg agttgacgag ttgaacagtt aatcctcact cactcagagc 135360gagagagcga gagagtggag gacgagttaa caagtcaatc ctcactcaga gtgagagagt 135420gagagagtgg aggacgagtt aatggttaac agttatcacc actcagagtg agagcggagg

135480acgagtcaac cactcgctcg cccactccga gttagagagg gaaccagtgc gagttaacgc 135540gcacacgagc gagagaacgt aaactcgctc gcgcgctcgc tcggctaacc gtcggcctct 135600cccaaaactc ttcgtaaaac tttcccgatg acagttcacc ctccaaaact ttgtaaaact 135660aaactgttcg gaggtcggtc tgctgcctct ctaactctcc gtaaaacgtt tgtaaactgt 135720cggaggtcgg tgacccgctc aaccgtccgc gaaaactttt cgcaggcagt gtctgcctct 135780ctcggactct ccgcaaacac tttcgcggaa cctcggaggg tggtcgacct ctctccaaac 135840tcttgcaaaa ctttttcgcg gaaccgttgg aggccagtcc tccctccaaa ctctttgtaa 135900aatcttttcg aggccagtcc tcctctccaa aacgttccgc aaaatctttg ggaggtcggc 135960ctctcctctc cagaacgttc cgtaaaactc ttggaggccg cccgcggcac gcgaggcgga 136020ggatccgagg tgtcgacctc cctcaaaaac tttgtaaaaa ctttttataa aactttccgc 136080ggaacctcgg agagtaggtc gacctctctc aaaactctta taaaactttt ccgcggaacc 136140gttggaaggt aggtcgacct ctctcaaaac tcttataaaa cttttccgcg gaaccgttgg 136200aaggtaggtc gacctctctc aaaactctta taaaactttt ccgcggaacc gttggaggca 136260ggtcggcctc tcaaactctt tgcgagaact cttcgcgaga actcttcgat aactttagga 136320ggtcaggtcg acctcccaaa acttttgcga gaactctctg taaaacttta ggaggtcggt 136380acctccctca aaacttttta taaaactttt cgcggaacct ccggagacgg gccgccgccg 136440cgaccgcggg agcggagagg ccgacctccc gagacgttcc gcgttaccgt cggggtaggc 136500gtcctctcga gaacgccaaa agacttcgtg caaaaacttt tcggaggggc gcggagggcg 136560ggtcagctcc cgcgaactcc cgcagaacct tttcgcgcga ccgcgaaggc cggccgcctc 136620tcccacactc tcaagagctt ttcggaggag aggaagggca ggtcgccccc acctccgacg 136680ctttgtaaaa acgtttacgc ggaacctcgg aggcaggtcg cctccctcga aaactcctcg 136740cgaaaccttt aaaaactttt tgcgaaaact tttcggagga tgtcggaggg cgggcggctc 136800ttccaaacct ccgcagaacc ttttcgcgca accgttggaa gacaggtcgg cctctctcga 136860aaacttttaa aactttgtaa acgcgttggc gggaccgtcg cgggagagcg gccgcccgcg 136920gcacgcgaga ggaggaaccg ttggaaggca gtcggcctct cccgaaaact ttttataaaa 136980acttttccgc ggaaccgttg gaggcaggtc ggcctctctc agagtctaaa aactttttgc 137040gggactcgga cggcgcggtc acccgaccac ctgactcctg tctcacccgt actacttgga 137100cttctgtttc cctgactccc gactccctga cctcccgact ccctgactcc cgactccctg 137160actcccgact ccctgactcc cgactccctg actcccgact ccctgactcc cgactccctg 137220actcccgact ccctgactcc cgactccctg actcccgact ccctgactcc cgactccctg 137280actcccgact ccctgactcc cgactccctg actcccgact ccctgactcc cgactccctg 137340actcccgact ccctgactcc ctgactccag agcgaggtct cgcggctgcg gggtgccgcc 137400tccgcggagt cgcgttcccg cggacgcccg tcctcgaaag cattcagcag ttccagcctc 137460tgccgtagct cctcccgcag gaactcctgg tccgcgttcg tcgcggcacc gcggctcagc 137520cgccgcggga gccggccgcc gcccgcgaag ccgcggatcc 137560223DNAArtificial SequencePrimer 2attacagtga tgcctacatg ccg 23319DNAArtificial SequencePrimer 3gctgtagtcg tggtccggc 19422DNAArtificial SequencePrimer 4cttcctaggc ttctaccgca cg 22523DNAArtificial SequencePrimer 5cggtttacgt tgaaatgtcc cat 23618DNAArtificial SequencePrimer 6ctggccaacg acgccttc 18718DNAArtificial SequencePrimer 7tctggtaccc cttgccgg 18818DNAArtificial SequencePrimer 8gaacccgctc tcgctcga 18919DNAArtificial SequencePrimer 9gccgggcaag tgtctggtc 191021DNAArtificial SequencePrimer 10ctcgaagtag ctgatgtcgc g 211128DNAArtificial SequencePrimer 11agagctttac gtagactctc caagtgtc 281222DNAArtificial SequencePrimer 12atacggaacg ggactatgga cg 221318DNAArtificial SequencePrimer 13gcggtggcca tgtacgtg 181418DNAArtificial SequencePrimer 14ggttgtggcg atggtcgg 181518DNAArtificial SequencePrimer 15cttgatgagc cggacgca 181621DNAArtificial SequencePrimer 16ccgagttgga gaggaaggag c 211723DNAArtificial SequencePrimer 17ctgttggagg atgaggtcaa gga 231819DNAArtificial SequencePrimer 18cgtgctcatg cctgtggac 191922DNAArtificial SequencePrimer 19cgacatcctc acctgcaaga ag 222018DNAArtificial SequencePrimer 20tacaggcagc ccgtgacc 182120DNAArtificial SequencePrimer 21gccgtgtgtc acgttgatgc 20226PRTArtificial SequenceSynthetic construct 22Ile Arg Gly Phe Ala Gly1 5237PRTArtificial SequenceSynthetic construct 23Pro Gln Lys Val Phe Arg Leu1 5247PRTArtificial SequenceSynthetic construct 24Met Ser Glu Gly Gly Arg Leu1 5257PRTArtificial SequenceSynthetic construct 25Leu Leu Gly Leu Leu Phe Pro1 5267PRTArtificial SequenceSynthetic construct 26Met Thr Val His Pro Pro Lys1 5277PRTArtificial SequenceSynthetic construct 27Val Leu Pro Pro Asn Ser Leu1 5287PRTArtificial SequenceSynthetic construct 28Met His Pro Ser Pro Arg Arg1 5297PRTArtificial SequenceSynthetic construct 29Pro Val Ser His Pro Phe Leu 1 5307PRTArtificial SequenceSynthetic construct 30Met Gly Asp Arg Glu Gly Glu1 5317PRTArtificial SequenceSynthetic construct 31Phe Glu Asp Gly Val Lys Cys1 5327PRTArtificial SequenceSynthetic construct 32Met Cys Thr Val Ala Thr Phe1 5337PRTArtificial SequenceSynthetic construct 33Gly Ala Pro Arg Ala Gly Trp1 5347PRTArtificial SequenceSynthetic construct 34Met Thr Pro Thr Ser Arg Glu1 5357PRTArtificial SequenceSynthetic construct 35Ala Arg Thr Ala Pro Pro Arg1 5367PRTArtificial SequenceSynthetic construct 36Met Pro Gly Glu Gly Gln Tyr1 5377PRTArtificial SequenceSynthetic construct 37Asn Gly Gly Leu Gly Lys Ile1 5387PRTArtificial SequenceSynthetic construct 38Met Glu Phe Cys His Thr Glu1 5397PRTArtificial SequenceSynthetic construct 39Asp Thr Ala Trp Tyr Ile Ser1 5407PRTArtificial SequenceSynthetic construct 40Met Leu Ser Arg Glu Ser Val1 5417PRTArtificial SequenceSynthetic construct 41Arg Ala Met Leu Thr Arg Pro1 5427PRTArtificial SequenceSynthetic construct 42Met Phe Phe Trp Phe Trp Cys1 5437PRTArtificial SequenceSynthetic construct 43Ser Gly Glu Gly Val Pro Val1 5447PRTArtificial SequenceSynthetic construct 44Met Leu Gly Phe Trp Gly Lys1 5457PRTArtificial SequenceSynthetic construct 45Val Leu Pro Ser Val Ser Arg1 5467PRTArtificial SequenceSynthetic construct 46Met Trp Pro Phe Ser Ser Ile 1 5477PRTArtificial SequenceSynthetic construct 47Glu Phe Cys Lys Pro Ile Asn 1 5487PRTArtificial SequenceSynthetic construct 48Met Leu Ile Tyr Gly Pro Arg 1 5497PRTArtificial SequenceSynthetic construct 49Arg Leu Leu Lys Asp Phe Pro 1 5507PRTArtificial SequenceSynthetic construct 50Met Gly Val Val Met Cys Gly 1 5517PRTArtificial SequenceSynthetic construct 51Ala Pro Ala Gly Val Thr Glu 1 5527PRTArtificial SequenceSynthetic construct 52Met Pro Val Lys Val Lys Gln1 5537PRTArtificial SequenceSynthetic construct 53Ala Ser Arg Glu Phe Ile Val 1 5547PRTArtificial SequenceSynthetic construct 54Met Glu Glu Glu Leu Thr Arg 1 5557PRTArtificial SequenceSynthetic construct 55Ser Pro Met Val Val Phe Asn 1 5567PRTArtificial SequenceSynthetic construct 56Met Ile Arg Ile Gly Gly Gly 1 5577PRTArtificial SequenceSynthetic construct 57Asp Asn Met Arg Val Asp Asp 1 5587PRTArtificial SequenceSynthetic construct 58Met Asp Gly Gly Val His Lys 1 5597PRTArtificial SequenceSynthetic construct 59Glu Gln Met Cys Arg Arg Gln 1 5607PRTArtificial SequenceSynthetic construct 60Met Ala Pro Pro Val Ile Glu 1 5617PRTArtificial SequenceSynthetic construct 61Ala Lys Asn Val Ile Thr His1 5627PRTArtificial SequenceSynthetic construct 62Met Leu Gln Leu Leu Lys Arg 1 5637PRTArtificial SequenceSynthetic construct 63Asn Asn Arg Gly Phe Arg Lys 1 5647PRTArtificial SequenceSynthetic construct 64Met Ala Cys Glu Cys Ala Ser 1 5657PRTArtificial SequenceSynthetic construct 65Asn Asn Cys Gly Ile Ser Phe 1 5667PRTArtificial SequenceSynthetic construct 66Met Asp Glu Asp Arg Leu Arg 1 5677PRTArtificial SequenceSynthetic construct 67Lys Lys Gly Lys Pro Lys Ser 1 5687PRTArtificial SequenceSynthetic construct 68Met Asp Phe Val Arg Arg Lys 1 5697PRTArtificial SequenceSynthetic construct 69Val Val Leu Gln Gly Arg Ala 1 5707PRTArtificial SequenceSynthetic construct 70Met Val Asp Ser Gly Thr His 1 5717PRTArtificial SequenceSynthetic construct 71Pro Glu Asn Val Val Leu Leu 1 5727PRTArtificial SequenceSynthetic construct 72Met Ala Ser Tyr Ile Ser Gly 1 5737PRTArtificial SequenceSynthetic construct 73Arg Thr His Thr Val Tyr Val 1 5747PRTArtificial SequenceSynthetic construct 74Met Leu Phe Glu Met Glu Leu 1 5757PRTArtificial SequenceSynthetic construct 75Ser Lys Pro Val Phe Thr Gly 1 5767PRTArtificial SequenceSynthetic construct 76Met Glu Pro Arg Phe Trp Gly 1 5777PRTArtificial SequenceSynthetic construct 77Ala Lys Val Arg Pro Leu Val 1 5787PRTArtificial SequenceSynthetic construct 78Met Glu Ala Ile Asn Val Phe 1 5797PRTArtificial SequenceSynthetic construct 79Arg Ala Tyr Glu Gly Met Leu 1 5807PRTArtificial SequenceSynthetic construct 80Met Leu Leu Tyr Pro Lys Lys 1 5817PRTArtificial SequenceSynthetic construct 81Leu Leu Gly Asp Gly Gly Asp 1 5827PRTArtificial SequenceSynthetic construct 82Met Leu Ile Arg Thr Thr Asp 1 5837PRTArtificial SequenceSynthetic construct 83Glu Ala Gln Asn Met Gln Asn 1 5847PRTArtificial SequenceSynthetic construct 84Met Glu Asp Glu Arg Leu Ile 1 5857PRTArtificial SequenceSynthetic construct 85Pro Ser Pro Cys Gly Gly Glu 1 5867PRTArtificial SequenceSynthetic construct 86Met Asp Lys Leu Tyr Thr Gly 1 5877PRTArtificial SequenceSynthetic construct 87Phe His Tyr Leu Lys Leu Val 1 5887PRTArtificial SequenceSynthetic construct 88Met Lys Arg Ala Val Ser Lys 1 5897PRTArtificial SequenceSynthetic construct 89Leu Glu Ala Pro Phe Asn Ile 1 5907PRTArtificial SequenceSynthetic construct 90Met Glu Ser Arg Asp Leu Gly 1 5917PRTArtificial SequenceSynthetic construct 91Leu Asn Ala Arg Arg Gln Asn 1 5927PRTArtificial SequenceSynthetic construct 92Met Asn His Phe Phe Lys Gln 1 5937PRTArtificial SequenceSynthetic construct 93Arg Ser Leu Tyr Thr Val Leu 1 5947PRTArtificial SequenceSynthetic construct 94Met Asp Lys Tyr Thr Asp Leu 1 5957PRTArtificial SequenceSynthetic construct 95Pro Glu Lys Pro Ala Ala Pro 1 5967PRTArtificial SequenceSynthetic construct 96Met Glu Asn His Leu Pro Asp1 5977PRTArtificial SequenceSynthetic construct 97Ile Glu Ala Glu Pro Pro Phe1 5987PRTArtificial SequenceSynthetic construct 98Met Ile Val Leu Glu Asn Gly 1 5997PRTArtificial SequenceSynthetic construct 99Arg Met Gly Ala Arg Pro Arg 1 51007PRTArtificial SequenceSynthetic construct 100Met Thr Phe Arg Glu Leu Ile 1 51017PRTArtificial SequenceSynthetic construct 101Asp Ser Met Ala Ser Arg Ser 1 51027PRTArtificial SequenceSynthetic construct 102Met Arg Gly His Pro Ala His 1 51037PRTArtificial SequenceSynthetic construct 103Val Ala Pro Arg Glu Glu Leu1 51047PRTArtificial SequenceSynthetic construct 104Met Ala Ser Asp Ala Ser Pro 1 51057PRTArtificial SequenceSynthetic construct 105Gln Pro Ser Ser Ser Arg Arg 1 51067PRTArtificial SequenceSynthetic construct 106Met Gly Ile Lys Asn Leu Lys 1 51077PRTArtificial SequenceSynthetic construct 107Pro Arg Leu Leu Lys Leu Arg 1 51087PRTArtificial SequenceSynthetic construct 108Met Val Phe Pro Ile Val Cys 1 51097PRTArtificial SequenceSynthetic construct 109Leu Pro Met Leu Asp Ile Ser 1 51107PRTArtificial SequenceSynthetic construct 110Met Arg Glu Phe Gly Leu Ala 1 51117PRTArtificial SequenceSynthetic construct 111Ala Glu Pro Pro Trp Leu Val 1 51127PRTArtificial SequenceSynthetic construct 112Met Glu Ser Ser Lys Gln Ala 1 51137PRTArtificial SequenceSynthetic construct 113Thr Arg Ala Pro Pro Leu Phe 1 51147PRTArtificial SequenceSynthetic construct 114Met Thr Leu Arg Ile Lys Leu 1 51157PRTArtificial SequenceSynthetic construct 115Asp Arg Ser Leu Ser Cys Asp 1 51167PRTArtificial SequenceSynthetic construct 116Met Gly Gly Ser Val Ser Leu 1 51177PRTArtificial SequenceSynthetic construct 117Tyr Leu Leu Ile Val Trp Leu 1 51187PRTArtificial SequenceSynthetic construct 118Met Gly Ala Ala Ala Ser Ile 1 51197PRTArtificial SequenceSynthetic construct 119Thr Glu Phe Pro Pro Ser Val 1 51207PRTArtificial SequenceSynthetic construct 120Met Val Arg Arg Val Leu Leu 1 51217PRTArtificial SequenceSynthetic construct 121Leu Cys Leu Phe Ser Met Asp 1 51227PRTArtificial SequenceSynthetic construct 122Met Glu Glu Lys Arg Gly Arg 1 51237PRTArtificial SequenceSynthetic construct 123Ala Arg Ala Met Val Cys Leu 1 51247PRTArtificial SequenceSynthetic construct 124Met Thr Asn Leu Leu Ser Leu 1 51257PRTArtificial SequenceSynthetic construct 125Thr Gly Ala Glu Ala Ala Pro 1 51267PRTArtificial SequenceSynthetic construct 126Met Ala Ala Pro Thr Thr Pro 1 51277PRTArtificial SequenceSynthetic construct 127Val Asp Val Leu Gly Gly Arg 1 51287PRTArtificial SequenceSynthetic construct 128Met Asp His Glu Lys Tyr Val 1 51297PRTArtificial SequenceSynthetic construct 129Ala Thr Leu Ser Pro Gly Leu 1 51307PRTArtificial SequenceSynthetic construct 130Met Glu Gly Val Glu Met Asp 1 51317PRTArtificial SequenceSynthetic construct 131Arg Pro Leu Arg Gly Gly Lys 1 51327PRTArtificial SequenceSynthetic construct 132Met Asn Arg His Asn Thr Arg 1 51337PRTArtificial SequenceSynthetic construct 133Ser Val Ser Val Val Leu Asp 1 51347PRTArtificial SequenceSynthetic construct 134Met Phe Phe Arg Arg Arg Ala 1 51357PRTArtificial SequenceSynthetic construct 135Gly Arg Arg Pro Pro Arg Pro 1 51367PRTArtificial SequenceSynthetic construct 136Met Ser Val Val Ala Arg Val1 51377PRTArtificial SequenceSynthetic construct 137Glu Ala Ala Glu Glu Glu Phe 1 51387PRTArtificial SequenceSynthetic construct 138Met Gly Asp Lys Ser Glu Trp 1 51397PRTArtificial SequenceSynthetic construct 139Phe Val Cys Asp Ser Pro Ser 1 51407PRTArtificial SequenceSynthetic construct 140Met Ala Ala Ala Pro Leu Arg 1 51417PRTArtificial SequenceSynthetic construct 141Ala Thr Ser Gly Val Leu Thr 1 51427PRTArtificial SequenceSynthetic construct 142Met Asp Pro Pro Glu Ile Thr 1 51437PRTArtificial SequenceSynthetic construct 143Leu Leu Val Thr Ala Ile Val 1 51447PRTArtificial SequenceSynthetic construct 144Met Asp Ser Arg Glu Ser Ile 1 51457PRTArtificial SequenceSynthetic construct 145Tyr Met Ile Asn Phe Asn Asn 1 51467PRTArtificial SequenceSynthetic construct 146Met Ser Ser Trp Arg Leu Lys 1 51477PRTArtificial SequenceSynthetic construct 147Lys Ala Ala Ala Cys Lys Lys 1 51487PRTArtificial SequenceSynthetic construct 148Met Arg Ala Leu His Leu Ser 1 51497PRTArtificial SequenceSynthetic construct 149Asn Ser Glu Gln Val Asn Gly1 51507PRTArtificial SequenceSynthetic construct 150Met Asp Glu Ala Leu Arg Val 1 51517PRTArtificial SequenceSynthetic construct 151Phe Ile Arg Ala Ala Val Ala 1 51527PRTArtificial SequenceSynthetic construct 152Met Asp Ala Pro Ser Leu Asp 1 51537PRTArtificial SequenceSynthetic construct 153Leu Tyr Val Phe Ser Lys Arg 1 51547PRTArtificial SequenceSynthetic construct 154Met Glu Pro Ser Ala Met Arg 1 51557PRTArtificial SequenceSynthetic construct 155Asp Val Gln His Val Asp Leu 1 51567PRTArtificial SequenceSynthetic construct 156Met Ala Gly Phe Ser Gln Ser 1 51577PRTArtificial SequenceSynthetic construct 157Thr Thr Cys Val Pro Pro Gln 1 51587PRTArtificial SequenceSynthetic construct 158Met Ala Thr Pro Ala Asn Ala 1 51597PRTArtificial SequenceSynthetic construct 159Phe Ser Phe Tyr Ser Glu Asn 1 51607PRTArtificial SequenceSynthetic construct 160Met Ala Ala Pro Ile Cys Asp 1 51617PRTArtificial SequenceSynthetic construct 161Ile Glu Asp Val Glu

Asn Lys 1 51627PRTArtificial SequenceSynthetic construct 162Met Asn Ser Asp Val Ile Lys 1 51637PRTArtificial SequenceSynthetic construct 163Glu Val Ser Val Val Asn Ile 1 51647PRTArtificial SequenceSynthetic construct 164Met Ser Thr Phe Arg Gln Thr 1 51657PRTArtificial SequenceSynthetic construct 165Ala Ser Pro Ala Ala Lys Asn 1 51667PRTArtificial SequenceSynthetic construct 166Met Arg Thr Tyr Thr Ser Leu 1 51677PRTArtificial SequenceSynthetic construct 167Trp Gly Ala Ala Val Thr Arg 1 51687PRTArtificial SequenceSynthetic construct 168Met Thr Ser Ala His Ala Ala 1 51697PRTArtificial SequenceSynthetic construct 169Val Asp Pro Ala Ser Ile Ala 1 51707PRTArtificial SequenceSynthetic construct 170Met Glu Gly Arg Ala Arg Phe 1 51717PRTArtificial SequenceSynthetic construct 171Arg Phe Cys Asn Tyr Cys Pro 1 51727PRTArtificial SequenceSynthetic construct 172Met Lys Thr Asp Cys Ala Ser1 51737PRTArtificial SequenceSynthetic construct 173Lys Leu Lys Leu Leu Leu Gln 1 51747PRTArtificial SequenceSynthetic construct 174Met Asn Asn Ser Val Val Ser 1 51757PRTArtificial SequenceSynthetic construct 175Ala Glu Lys Val Thr Ala Gln 1 51767PRTArtificial SequenceSynthetic construct 176Met Lys Arg Ile Ala Leu Ser 1 51777PRTArtificial SequenceSynthetic construct 177Met Ala Leu Lys Ser Leu Ile 1 51787PRTArtificial SequenceSynthetic construct 178Met Asn Leu Arg Met Cys Gly 1 51797PRTArtificial SequenceSynthetic construct 179Ala Ala Cys Ser Leu Asp Leu 1 51807PRTArtificial SequenceSynthetic construct 180Met Gly Asp Asn Val Trp Phe 1 51817PRTArtificial SequenceSynthetic construct 181Val Leu Gly Leu Glu Gln Ala1 51827PRTArtificial SequenceSynthetic construct 182Met Glu Ser Pro Ala Cys Ala 1 51837PRTArtificial SequenceSynthetic construct 183Asn Met Cys Asp Val Leu Cys 1 51847PRTArtificial SequenceSynthetic construct 184Met Asp Leu Arg Arg Arg Phe 1 51857PRTArtificial SequenceSynthetic construct 185Val Asp Asn Thr Gly Thr Ser 1 51867PRTArtificial SequenceSynthetic construct 186Met Glu Glu Ser Val Ala Val 1 51877PRTArtificial SequenceSynthetic construct 187Leu Leu Asn Tyr Gly Cys Gly 1 51887PRTArtificial SequenceSynthetic construct 188Met Asp Arg Leu Arg Thr Cys 1 51897PRTArtificial SequenceSynthetic construct 189Ala Glu Ala Ala Glu Ser Ala 1 51907PRTArtificial SequenceSynthetic construct 190Met Val Ser Val Met Arg Lys 1 51917PRTArtificial SequenceSynthetic construct 191Gln Glu Phe Tyr Pro Gln Pro 1 51927PRTArtificial SequenceSynthetic construct 192Met Phe Gln Pro Val Pro Asp 1 51937PRTArtificial SequenceSynthetic construct 193Ser Ala Cys Arg Ala Ser Pro 1 51947PRTArtificial SequenceSynthetic construct 194Met Arg Pro Cys Tyr Val Thr 1 51957PRTArtificial SequenceSynthetic construct 195Thr Arg Gly Thr Gln Thr Gly 1 51967PRTArtificial SequenceSynthetic construct 196Met Thr Ala Pro Asn Val His 1 51977PRTArtificial SequenceSynthetic construct 197Ala Val Ser Phe Asp Ser Glu 1 51987PRTArtificial SequenceSynthetic construct 198Met Thr Ala Val Pro Val Thr 1 51997PRTArtificial SequenceSynthetic construct 199Val Arg Lys Leu Asn Leu Ile 1 52007PRTArtificial SequenceSynthetic construct 200Met Ala Ser Glu Lys Met Ala 1 52017PRTArtificial SequenceSynthetic construct 201Asp Leu Asp Gly Gly Met Cys 1 52027PRTArtificial SequenceSynthetic construct 202Met Gly Leu Leu Asp Ala Leu 1 52037PRTArtificial SequenceSynthetic construct 203Arg Phe Ser Ala Ala Ser Ser 1 52047PRTArtificial SequenceSynthetic construct 204Met Asp Ile Phe Glu Thr Leu 1 52057PRTArtificial SequenceSynthetic construct 205Asp Ile Glu Leu Thr Ala Arg 1 52067PRTArtificial SequenceSynthetic construct 206Met Val Ser Asp Tyr Asp Pro 1 52077PRTArtificial SequenceSynthetic construct 207His Phe Val His Ser Val Ile 1 52087PRTArtificial SequenceSynthetic construct 208Met Phe Leu Asp Ser Asp Thr 1 52097PRTArtificial SequenceSynthetic construct 209Asp Met Pro Phe Ser Val Val 1 52107PRTArtificial SequenceSynthetic construct 210Met Gly Asp Thr Val Ser Lys 1 52117PRTArtificial SequenceSynthetic construct 211Lys Thr Ile Asn Val Ser Arg 1 52127PRTArtificial SequenceSynthetic construct 212Met Glu Ser Tyr Phe Ser Tyr 1 52137PRTArtificial SequenceSynthetic construct 213Glu Asp Leu Phe Phe Ala Glu 1 52147PRTArtificial SequenceSynthetic construct 214Met Phe Gly Gly Val Gln Val 1 52157PRTArtificial SequenceSynthetic construct 215Gly Arg Asp Leu Ala Ala Val 1 52167PRTArtificial SequenceSynthetic construct 216Met Ser Ala Val Lys Ala Lys 1 52177PRTArtificial SequenceSynthetic construct 217Pro Leu Arg Asp Leu Ala Arg 1 52187PRTArtificial SequenceSynthetic construct 218Met Thr Ser Glu Ser Asp Leu 1 52197PRTArtificial SequenceSynthetic construct 219Ala Ile Ala Arg Ala Gln Pro 1 52207PRTArtificial SequenceSynthetic construct 220Met Ile Val Ala Ala Phe Asp 1 52217PRTArtificial SequenceSynthetic construct 221Asn Tyr Val Leu Arg Thr Asn 1 52227PRTArtificial SequenceSynthetic construct 222Met Leu Ala Leu Phe Glu Phe 1 52237PRTArtificial SequenceSynthetic construct 223Leu Lys Glu Leu Leu Gly Pro 1 52247PRTArtificial SequenceSynthetic construct 224Met Glu Gln Ala Leu Gly Tyr1 52257PRTArtificial SequenceSynthetic construct 225Ser Leu Phe Ser Pro Glu Asp 1 52267PRTArtificial SequenceSynthetic construct 226Met Glu Ser Asp Asn Ala Leu 1 52277PRTArtificial SequenceSynthetic construct 227Gly Gln His Ala Ala Ile Trp 1 52287PRTArtificial SequenceSynthetic construct 228Met Glu Lys Leu Val Ser Asp 1 52297PRTArtificial SequenceSynthetic construct 229Gly Arg Ser Gly Ala Ile Trp 1 52307PRTArtificial SequenceSynthetic construct 230Met Asp Glu Asn Asp Gly Glu 1 52317PRTArtificial SequenceSynthetic construct 231Gln Thr Gly Tyr Ser Arg Tyr 1 52327PRTArtificial SequenceSynthetic construct 232Met Asp Ala Val Ser Ala Leu 1 52337PRTArtificial SequenceSynthetic construct 233Leu Phe Leu Lys Ser Ile Leu 1 52347PRTArtificial SequenceSynthetic construct 234Met Ala Asp Ala Pro Leu Val 1 52357PRTArtificial SequenceSynthetic construct 235Arg Glu Leu Arg Ala Asn Glu 1 52367PRTArtificial SequenceSynthetic construct 236Met Glu Glu Asp Leu Asn Glu 1 52377PRTArtificial SequenceSynthetic construct 237Met Gly Gln Ala Ser Ser Ala 1 52387PRTArtificial SequenceSynthetic construct 238Met Asp Val Val Gln Glu Val 1 52397PRTArtificial SequenceSynthetic construct 239Ala Asp Ser Asp Gly Gly Asn 1 52407PRTArtificial SequenceSynthetic construct 240Met Arg Ser Trp Phe Trp Gln 1 52417PRTArtificial SequenceSynthetic construct 241Pro Leu Thr Gly Met Cys Leu 1 52427PRTArtificial SequenceSynthetic construct 242Met Arg Pro Lys Ser Val Gly 1 52437PRTArtificial SequenceSynthetic construct 243Ser Gly His Thr Lys Pro Ser 1 52447PRTArtificial SequenceSynthetic construct 244Met Ala His Asn Thr Phe Glu 1 52457PRTArtificial SequenceSynthetic construct 245Lys Tyr Phe Cys Val Ser Asp 1 52467PRTArtificial SequenceSynthetic construct 246Met Gly Cys Cys Lys Val Pro 1 52477PRTArtificial SequenceSynthetic construct 247Cys Met Lys Glu Met His Gly 1 52487PRTArtificial SequenceSynthetic construct 248Met Ser Arg Leu Gln Ile Leu 1 52497PRTArtificial SequenceSynthetic construct 249Arg Lys Leu Asp Val Pro Ile 1 52507PRTArtificial SequenceSynthetic construct 250Met Lys Ala Val Leu Leu Leu 1 52517PRTArtificial SequenceSynthetic construct 251Leu Asn Leu Asn Pro Gly Asn 1 52527PRTArtificial SequenceSynthetic construct 252Met His Ala Ser Leu Ser Ser 1 52537PRTArtificial SequenceSynthetic construct 253Asp Glu Thr Leu Thr Tyr Arg 1 52547PRTArtificial SequenceSynthetic construct 254Met Glu Val Leu Val Ile Ile 1 52557PRTArtificial SequenceSynthetic construct 255Gly Glu Phe Phe Tyr Asp Glu 1 52567PRTArtificial SequenceSynthetic construct 256Met Pro Leu Phe Arg Lys Leu 1 52577PRTArtificial SequenceSynthetic construct 257Arg Asp Ala Leu Asp Gly Leu 1 52587PRTArtificial SequenceSynthetic construct 258Met Ala Cys Phe Ile Glu Leu 1 52597PRTArtificial SequenceSynthetic construct 259Thr Thr Phe Ser Ser Ser Glu 1 52609PRTArtificial SequenceSynthetic construct 260Met Ser Ser Ser Ser Ser Glu Thr Thr 1 52617PRTArtificial SequenceSynthetic construct 261Thr Thr Gly Thr Ser Thr Ser 1 52627PRTArtificial SequenceSynthetic construct 262Met Ala Cys Leu Arg Val Phe 1 52637PRTArtificial SequenceSynthetic construct 263Cys Ser Met Gln Thr Ala Arg 1 52647PRTArtificial SequenceSynthetic construct 264Met Ala Ile Ala His Thr Thr 1 52657PRTArtificial SequenceSynthetic construct 265Phe Arg Phe Arg Thr Pro Gly 1 52667PRTArtificial SequenceSynthetic construct 266Met Ala Ala Thr Ile Gln Ile 1 52677PRTArtificial SequenceSynthetic construct 267Lys Arg Asp Gly Tyr Ser Arg 1 52687PRTArtificial SequenceSynthetic construct 268Met Glu Gly Leu Met Pro Lys 1 52697PRTArtificial SequenceSynthetic construct 269Arg Pro Ile Ser Val Gln Lys 1 52707PRTArtificial SequenceSynthetic construct 270Met Asp Ser Arg Arg Leu Ala 1 52717PRTArtificial SequenceSynthetic construct 271Leu Gly Asp Ser Asp Ser Asp 1 52727PRTArtificial SequenceSynthetic construct 272Met Arg Leu Ile Leu Ala Leu 1 52737PRTArtificial SequenceSynthetic construct 273Pro Gln Met Met Arg Ile Gly 1 52747PRTArtificial SequenceSynthetic construct 274Met Ala Gly Phe Leu Gly Ala 1 52757PRTArtificial SequenceSynthetic construct 275Cys Lys Val Glu Glu Val Leu 1 52767PRTArtificial SequenceSynthetic construct 276Met His Leu His Lys Asp Pro 1 52777PRTArtificial SequenceSynthetic construct 277Leu Ala Phe Pro Ser Leu Ala 1 52787PRTArtificial SequenceSynthetic construct 278Met Ala Asn Arg Leu Val Phe 1 52797PRTArtificial SequenceSynthetic construct 279Arg Pro Met Glu Ile Asp Gly 1 52807PRTArtificial SequenceSynthetic construct 280Met Glu Asn Asn Asp Gly Asn 1 52817PRTArtificial SequenceSynthetic construct 281Arg Phe Leu Pro Ser His Lys 1 52827PRTArtificial SequenceSynthetic construct 282Met Asp Pro Ala Gly Gln Arg 1 52837PRTArtificial SequenceSynthetic construct 283Cys Ser Glu Thr Asp Arg Trp 1 52847PRTArtificial SequenceSynthetic construct 284Met Ser Ser Ser Ala Ala Ala 1 52857PRTArtificial SequenceSynthetic construct 285Ile Ala Pro Asp Ser Arg Met 1 52867PRTArtificial SequenceSynthetic construct 286Met Thr Ala Glu Ala Ser Ile 1 52877PRTArtificial SequenceSynthetic construct 287Asp Pro Val Tyr His Lys Lys 1 52887PRTArtificial SequenceSynthetic construct 288Met Pro Arg Thr Thr Ser Gly 1 52897PRTArtificial SequenceSynthetic construct 289Arg Glu Gln Thr Glu Gly Leu 1 52907PRTArtificial SequenceSynthetic construct 290Met Ala Asn Arg Glu Glu Ile 1 52917PRTArtificial SequenceSynthetic construct 291Val Arg Val Leu Arg Arg Thr 1 52927PRTArtificial SequenceSynthetic construct 292Met Thr Ala Pro Thr Pro Arg 1 52937PRTArtificial SequenceSynthetic construct 293Ala Ala Tyr Ser Leu Ala Arg 1 52947PRTArtificial SequenceSynthetic construct 294Met Ala Asp Glu Arg Glu Ala 1 52957PRTArtificial SequenceSynthetic construct 295Leu Ala Cys Ala Met Arg Lys 1 52967PRTArtificial SequenceSynthetic construct 296Met Ser Lys Asn Lys Ile Leu 1 52977PRTArtificial SequenceSynthetic construct 297Ser Tyr Met Thr Thr Lys Met 1 52987PRTArtificial SequenceSynthetic construct 298Met Leu Thr Arg Cys Tyr Ile 1 52997PRTArtificial SequenceSynthetic construct 299Arg Ala Ser Gly Leu Ala Glu 1 53007PRTArtificial SequenceSynthetic construct 300Met Val Gly Phe Asp Arg Arg 1 53017PRTArtificial SequenceSynthetic construct 301Cys Gly Arg Arg Ala Pro Glu 1 53027PRTArtificial SequenceSynthetic construct 302Met Ile Leu Ala Arg Ala Gly 1 53037PRTArtificial SequenceSynthetic construct 303Pro Asp Ala Ala Ala Leu Ser 1 53047PRTArtificial SequenceSynthetic construct 304Met Pro Pro Arg Thr Pro Pro 1 53057PRTArtificial SequenceSynthetic construct 305Arg Pro Ala Ala Leu Arg Ala 1 53067PRTArtificial SequenceSynthetic construct 306Met Lys Leu Leu Val Gly Ile 1 53077PRTArtificial SequenceSynthetic construct 307Arg Pro Pro Arg Arg Arg Arg 1 53087PRTArtificial SequenceSynthetic construct 308Met Arg Lys Lys Ala Pro Arg 1 53097PRTArtificial SequenceSynthetic construct 309Ala Arg Thr Ala Pro Pro Arg 1 53107PRTArtificial SequenceSynthetic construct 310Met Met Arg Ser Gly His Ala 1 53117PRTArtificial SequenceSynthetic construct 311Arg Met His Arg Ser Glu Leu 1 53127PRTArtificial SequenceSynthetic construct 312Met Cys Thr Val Ala Thr Phe 1 53137PRTArtificial SequenceSynthetic construct 313Ser Val Ala Pro Ser Ser Ala 1 53147PRTArtificial SequenceSynthetic construct 314Met Thr Val His Pro Pro Lys 1 53157PRTArtificial SequenceSynthetic construct 315Val Leu Pro Pro Asn Ser Leu 1 53167PRTArtificial SequenceSynthetic construct 316Met Ser Glu Gly Gly Arg Leu 1 53177PRTArtificial SequenceSynthetic construct 317Leu Leu Gly Leu Leu Phe Pro 1 53187PRTArtificial SequenceSynthetic construct 318Ile Arg Gly Phe Ala Gly Gly 1 53197PRTArtificial SequenceSynthetic construct 319Pro Gln Lys Val Phe Arg Leu 1 53208PRTArtificial SequenceSynthetic construct 320Ser Ile Ile Asn Phe Glu Lys Leu 1 5

Claims

1. A method for inducing an immunomodulatory activity in a subject, consisting of administering to the subject an individual recombinant protein encoded by a polynucleotide selected from the group consisting of: (i) a polynucleotide having the sequence consisting of nucleotide residues 31003 to 46845 of SEQ ID NO:1 (PPVO insert of VVOV 96), (ii) a polynucleotide having the sequence consisting of nucleotide residues 24056 to 33789 of SEQ ID NO:1 (PPVO insert of VVOV 97), (iii) a polynucleotide having the sequence consisting of nucleotide residues 82324 to 92502 of SEQ ID NO:1 (PPVO insert of VVOV 243), (iv) a polynucleotide having the sequence consisting of nucleotide residues 74804 to 88576 of SEQ ID NO:1 (PPVO insert of VVOV 285), and (v) a polynucleotide having the sequence consisting of nucleotide residues 102490 to 108393 of SEQ ID NO:1 (PPVO insert of VVOV 330).

2. The method of claim 1, wherein the immunomodulatory activity results in the induction of interferon-gamma.

3. The method of claim 1, wherein the immunomodulatory activity results in the induction of tumor necrosis factor-alpha.

4. The method of claim 1, wherein the immunomodulatory activity results in the stimulation of MHC-I cross-presentation.

5. The method of claim 1, wherein the recombinant protein is attached to or is a part of a structure selected from the group consisting of: particle-like structure, fusion protein, protein coated particle, and virus-like particle.

6. A method for inducing an immunomodulatory activity in a subject, consisting of administering to the subject an individual recombinant virus containing a recombinant protein encoded by a polynucleotide selected from the group consisting of: (i) a polynucleotide having the sequence consisting of nucleotide residues 31003 to 46845 of SEQ ID NO:1 (PPVO insert of VVOV 96), (ii) a polynucleotide having the sequence consisting of nucleotide residues 24056 to 33789 of SEQ ID NO:1 (PPVO insert of VVOV 97), (iii) a polynucleotide having the sequence consisting of nucleotide residues 82324 to 92502 of SEQ ID NO:1 (PPVO insert of VVOV 243), (iv) a polynucleotide having the sequence consisting of nucleotide residues 74804 to 88576 of SEQ ID NO:1 (PPVO insert of VVOV 285), and (v) a polynucleotide having the sequence consisting of nucleotide residues 102490 to 108393 of SEQ ID NO:1 (PPVO insert of VVOV 330).

7. The method of claim 6 wherein the immunomodulatory activity results in the induction of interferon-gamma.

8. The method of claim 6 wherein the immunomodulatory activity results in the induction of tumor necrosis factor-alpha.

9. The method of claim 6 wherein the immunomodulatory activity results in the stimulation of MHC-I cross-presentation.

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