Molecular Markers Associated with Soybean Tolerance to Low Iron Growth Conditions

Abstract

The present invention provides methods and compositions for identifying soybean plants that are tolerant or have improved tolerance, or those that are susceptible to, iron deficient growth conditions. The methods use molecular markers to identify, select, and/or introgress genetic loci modulating phenotypic expression of an iron deficiency tolerance trait in soybean plant breeding. Methods are provided for screening germplasm entries for the performance and expression of this trait.

Description

CROSS REFERENCE TO RELATED APPLICATION

[0001] This application claims the benefit of priority to U.S. Provisional Application Ser. No. 61/833,129 filed Jun. 10, 2013.

INCORPORATION OF SEQUENCE LISTING

[0002] A sequence listing containing the file named "46-21-59627.txt" which is 99,843 bytes (measured in MS-Windows®) and created on May 30, 2014, comprises "147" nucleotide sequences, is provided herewith via the USPTO's EFS system and is herein incorporated by reference in its entirety.

BACKGROUND OF INVENTION

[0003] Soybean, Glycine max (L.) Merril, is a major economic crop worldwide and is a primary source of vegetable oil and protein (Sinclair and Backman, Compendium of Soybean Diseases, 3rd Ed. APS Press, St. Paul, Minn., p. 106. (1989). Growing demand for low cholesterol and high fiber diets has increased soybean's importance as a health food.

[0004] Soybean varieties grown in the United States have a narrow genetic base. Six introductions, `Mandarin,` `Manchu,` `Mandarin` (Ottawa), `Richland,` `AK` (Harrow), and `Mukden,` contributed nearly 70% of the germplasm represented in 136 cultivar releases. To date, modern day cultivars can be traced back from these six soybean strains from China. In a study conducted by Cox et al., Crop Sci. 25:529-532 (1988), the soybean germplasm is comprised of 90% adapted materials, 9% un-adapted, and only 1% from exotic species. The genetic base of cultivated soybean could be widened through exotic species. In addition, exotic species may possess such key traits as disease, stress, and insect resistance.

[0005] The availability of a specific micronutrient, such as iron (Fe), is often related to soil characteristics. Soil pH has a major impact on the availability of Fe. Iron deficiency has been a common, serious, and yield-limiting problem for soybean production in some parts of the United States.

[0006] Iron is one of the necessary micronutrients for soybean plant growth and development. It is also needed for the development of chlorophyll. Iron is involved in energy transfer, plant respiration, and plant metabolism. It is also a constituent of certain enzymes and proteins in plants. Iron is necessary for soybean root nodule formation and plays a role in N-fixation; thus, low levels of Fe can lead to reduced nitrogen content and poor yield.

[0007] When iron is limited, soybean plants can develop iron deficiency chlorosis (IDC). Soybean IDC is the result of a complex interaction among many factors including soil chemistry, environmental conditions, and soybean physiology and genetics. The most common IDC symptom is interveinal chlorosis in which leaf tissue of newly developed soybean leaves turn yellow, while the veins remain green. The leaves may develop necrotic spots that eventually coalesce and fall off the plant. Iron deficiency symptoms are similar to that of Manganese (Mn) deficiency; therefore, only soil and tissue analysis can distinguish the two micronutrient deficiencies.

[0008] Severe yield reductions due to IDC have been reported throughout the North-Central U.S with losses estimated to be around $120 million annually. In some instances, yield loss can be greater than 50%. Typically, soybean IDC symptoms occur between the first and third trifoliate stage, so under less-severe iron deficiency conditions, symptoms may improve later in the season.

[0009] Soybean plants grown in calcareous soils with a pH of greater than 7.4 or in heavy, poorly drained, and compacted soils may exhibit IDC symptoms due to insufficient iron uptake. However, soil pH is not a good indicator and does not correlate very well with IDC. Symptoms are highly variable between years and varieties and depend on other soil factors and weather conditions.

[0010] There is, however, a direct relationship between IDC incidence and concentrations of calcium carbonate and soluble salts. Iron uptake is adversely impacted by high concentrations of phosphorous (P), manganese (Mn), and zinc (Zn). Moreover, high levels of calcium (Ca) in the soil cause Fe molecules to bind tightly to the soil particles, making them unavailable for uptake. Therefore it is important to monitor the levels of calcium carbonate and soluble salts in the soil. Sandy soils with low organic matter may also lead to a greater incidence of IDC symptoms.

[0011] Weather also plays a role in IDC symptoms. Cool soil temperature and wet weather, combined with marginal levels of available Fe in the soil can increase IDC symptoms.

[0012] Soybean producers have sought to develop plants tolerant to low iron growth conditions (thus not exhibiting IDC) as a cost-effective alternative or supplement to standard foliar, soil and/or seed treatments (e.g., Hintz et al. (1987) "Population development for the selection of high-yielding soybean cultivars with resistance to iron deficiency chlorosis," Crop Sci. 28:369-370). Studies also suggest that cultivar selection is more reliable and universally applicable than foliar sprays or iron seed treatment methods, though environmental and cultivar selection methods can also be used effectively in combination (Goos and Johnson (2000) "A Comparison of Three Methods for Reducing Iron-Deficiency Chlorosis in Soybean" Agronomy Journal 92:1135-1139; and Goos and Johnson "Seed Treatment, Seeding Rate, and Cultivar Effects on Iron Deficiency Chlorosis of Soybean" Journal of Plant Nutrition 24 (8) 1255-1268).

[0013] Soybean cultivar improvement for IDC tolerance can be performed using classical breeding methods, or, more preferably, using marker assisted selection (MAS). Genetic markers for low iron growth condition tolerance/susceptibility have been identified (e.g., Lin et al. (2000) "Molecular characterization of iron deficiency chlorosis in soybean" Journal of Plant Nutrition 23:1929-1939). Recent work suggests that marker assisted selection is particularly beneficial when selecting plants because the strength of environmental effects on chlorosis expression impedes progress in improving tolerance. See also, Charlson et al., "Associating SSR Markers with Soybean Resistance to Iron Chlorosis," Journal of Plant Nutrition, vol. 26, nos. 10 & 11; 2267-2276 (2003). U.S. Pat. Nos. 7,977,533 and 7,582,806 disclose genetic loci associated with iron deficiency tolerance in soybean.

[0014] There is a need in the art of plant breeding to identify additional markers linked to genomic regions associated with tolerance to low iron growth conditions (e.g., IDC tolerance) in soybean. There is, in particular, a need for numerous markers that are closely associated with low iron growth condition tolerance in soybean that permit introgression of such regions in the absence of extraneous linked DNA from the source germplasm containing the regions. Additionally, there is a need for rapid, cost-efficient methods to assay the absence or presence of IDC tolerance loci in soybean.

SUMMARY OF INVENTION

[0015] The present invention provides for methods of creating a population of soybean plants with a low iron growth condition tolerant phenotype, comprising a.) providing a first population of soybean plants; b.) detecting in said soybean plant an allele in at least one polymorphic nucleic acid marker locus associated with the low iron growth condition tolerant phenotype wherein the marker locus genetically linked by less than 20 cM to a linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075, linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010, linkage group M genomic region flanked by loci TA75172_--3847 and TC380682, linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870, or linkage group O genomic region flanked by loci NA and Cf16144d; c.) selecting said plant containing said allele to provide a plant having a genotype associated with a low iron growth condition tolerant phenotype; and d.) producing a population of offspring from at least on of said selected soybean plants.

[0016] In some embodiments of the invention, the marker locus is genetically linked by less than 15 cM to the linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075, linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010, linkage group M genomic region flanked by loci TA75172_--3847 and TC380682, linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870, or linkage group 0 genomic region flanked by loci NA and Cf16144d.

[0017] In some embodiments of the invention, the marker locus is genetically linked by less than 10 cM to the linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075, linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010, linkage group M genomic region flanked by loci TA75172_--3847 and TC380682, linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870, or linkage group 0 genomic region flanked by loci NA and Cf16144d.

[0018] In some embodiments of the invention a second marker locus associated with the low iron growth condition tolerant phenotype is in linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075.

[0019] In some embodiments of the invention a second marker locus associated with the low iron growth condition tolerant phenotype is in linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010.

[0020] In some embodiments of the invention a second marker locus associated with the low iron growth condition tolerant phenotype is in linkage group M genomic region flanked by loci TA75172_--3847 and TC380682.

[0021] In some embodiments of the invention a second marker locus associated with the low iron growth condition tolerant phenotype is in linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870.

[0022] In some embodiments of the invention a second marker locus associated with the low iron growth condition tolerant phenotype is in linkage group 0 genomic region flanked by loci NA and Cf16144d.

[0023] Also provided herein are methods for creating a population of soybean plants comprising at least one allele associated with the low iron growth phenotype comprising at least one of SEQ ID NOs: 1-147. In certain embodiments, these methods comprise a.) genotyping a first population of soybean plants, said population containing at least one allele associated with the low iron growth condition tolerant phenotype, the at least one allele associated with the low iron growth condition tolerant phenotype comprising at least one of SEQ ID NOS 1-147; b.) selecting from said first population one or more identified soybean plants containing said at least one allele associated with the low iron growth condition tolerant phenotype comprising at least one of SEQ ID NOS 1-147; and c.) producing from said selected soybean plants a second population, thereby creating a population of soybean plants comprising at least one allele associated with the low iron growth condition tolerant phenotype comprising at lest one of SEQ ID NOS 1-147.

[0024] Further areas of applicability will become apparent from the description provided herein. It should be understood that the description and specific examples are intended for purposes of illustration only and are not intended to limit the scope of the present disclosure.

DESCRIPTION OF INVENTION

I. Definitions

[0025] Unless otherwise indicated herein, nucleic acid sequences are written left to right in 5' to 3' orientation. Numeric ranges recited within the specification are inclusive of the numbers defining the range and include each integer or any non-integer fraction within the defined range. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention pertains.

[0026] As used herein, an "allele" refers to one of two or more alternative forms of a genomic sequence at a given locus on a chromosome. When all the alleles present at a given locus on a chromosome are the same, that plant is homozygous at that locus. If the alleles present at a given locus on a chromosome differ, that plant is heterozygous at that locus.

[0027] As used herein, the term "bulk" refers to a method of managing a segregating population during inbreeding that involves growing the population in a bulk plot, harvesting the self-pollinated seed of plants in bulk, and using a sample of the bulk to plant the next generation.

[0028] As used herein, the term "comprising" means "including but not limited to".

[0029] As used herein, the term "locus" refers to a position on a genomic sequence that is usually found by a point of reference; e.g., a short DNA sequence that is a gene, or part of a gene or intergenic region. A locus may refer to a nucleotide position at a reference point on a chromosome, such as a position from the end of the chromosome.

[0030] As used herein, "linkage group J" corresponds to the soybean linkage group J described in Choi, et al., Genetics. 2007 May; 176(1): 685-696. Linkage group J, as used herein, also corresponds to soybean chromosome 16 (as described on the World Wide Web at soybase.org/LG2Xsome.php).

[0031] As used herein, "linkage group E" corresponds to the soybean linkage group E described in Choi, et al., Genetics. 2007 May; 176(1): 685-696. Linkage group E, as used herein, also corresponds to soybean chromosome 15 (as described on the World Wide Web at soybase.org/LG2Xsome.php).

[0032] As used herein, "linkage group M" corresponds to the soybean linkage group M described in Choi, et al., Genetics. 2007 May; 176(1): 685-696. Linkage group M, as used herein, also corresponds to soybean chromosome 7 (as described on the World Wide Web at soybase.org/LG2Xsome.php).

[0033] As used herein, "linkage group D2" corresponds to the soybean linkage group D2 described in Choi, et al., Genetics. 2007 May; 176(1): 685-696. Linkage group D2, as used herein, also corresponds to soybean chromosome 17 (as described on the World Wide Web at soybase.org/LG2Xsome.php).

[0034] As used herein, "linkage group O" corresponds to the soybean linkage group 0 described in Choi, et al., Genetics. 2007 May; 176(1): 685-696. Linkage group 0, as used herein, also corresponds to soybean chromosome 10 (as described on the World Wide Web at soybase.org/LG2Xsome.php).

[0035] As used herein, "polymorphism" means the presence of one or more variations of a nucleic acid sequence at one or more loci in a population of at least two members. The variation can comprise, but is not limited to, one or more nucleotide base substitutions, the insertion of one or more nucleotides, a nucleotide sequence inversion, and/or the deletion of one or more nucleotides.

[0036] As used herein, "genotype" means the genetic component of the phenotype and it can be indirectly characterized using markers or directly characterized by nucleic acid sequencing.

[0037] As used herein, the term "introgressed," when used in reference to a genetic locus, refers to a genetic locus that has been introduced into a new genetic background. Introgression of a genetic locus can thus be achieved through both plant breeding methods or by molecular genetic methods. Such molecular genetic methods include, but are not limited to, various plant transformation techniques and/or methods that provide for homologous recombination, non-homologous recombination, site-specific recombination, and/or genomic modifications that provide for locus substitution or locus conversion. In certain embodiments, introgression could thus be achieved by substitution of a locus not associated with tolerance to low iron growth conditions with a corresponding locus that is associated with low iron growth condition tolerance or by conversion of a locus from a non-tolerant genotype to a tolerant genotype.

[0038] As used herein, "linkage" refers to relative frequency at which types of gametes are produced in a cross. For example, if locus A has genes "A" or "a" and locus B has genes "B" or "b," then a cross between parent 1 with AABB and parent 2 with aabb can produce four possible gametes segregating into AB, Ab, aB and ab genotypes. The null expectation is that there will be independent equal segregation into each of the four possible genotypes, i.e. no linkage between locus A and locus B results in 1/4 of the gametes from each genotype (AB, Ab, aB, and ab). Segregation of gametes into genotype ratios differing from 1/4 indicates linkage between locus A and locus B. As used herein, linkage can be between two markers, or alternatively between a marker and a phenotype. A marker locus can be associated with (linked to) a trait, e.g., a marker locus can be associated with tolerance or improved tolerance to a plant pathogen when the marker locus is in linkage disequilibrium (LD) with the tolerance trait. The degree of linkage of a molecular marker to a phenotypic trait (e.g., a QTL) is measured, e.g., as a statistical probability of co-segregation of that molecular marker with the phenotype.

[0039] As used herein, the linkage relationship between a molecular marker and a phenotype is given is the statistical likelihood that the particular combination of a phenotype and the presence or absence of a particular marker allele is random. Thus, the lower the probability score, the greater the likelihood that a phenotype and a particular marker will cosegregate. In some embodiments, a probability score of 0.05 (p=0.05, or a 5% probability) of random assortment is considered a significant indication of co-segregation. However, the present invention is not limited to this particular standard, and an acceptable probability can be any probability of less than 50% (p<0.5). For example, a significant probability can be less than 0.25, less than 0.20, less than 0.15, or less than 0.1.

[0040] As used herein, the term "linked" or "genetically linked," when used in the context of markers and/or genomic regions, means that recombination between two linked loci occurs with a frequency of equal to or less than about 10% (i.e., are separated on a genetic map by not more than 10 cM). In one aspect, any marker of the invention is linked (genetically and physically) to any other marker that is at or less than 50 cM distant. In another aspect, any marker of the invention is closely linked (genetically and physically) to any other marker that is in close proximity, e.g., at or less than 10 cM distant. Two closely linked markers on the same chromosome can be positioned 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5, 4, 3, 2, 1, 0.75, 0.5 or 0.25 cM or less from each other.

[0041] As used herein, "marker," "genetic marker," "molecular marker," and "marker locus" refer to a nucleotide sequence or encoded product thereof (e.g., a protein) used as a point of reference when identifying a linked locus. A marker can be derived from genomic nucleotide sequence or from expressed nucleotide sequences (e.g., from a spliced RNA, a cDNA, etc.), or from an encoded polypeptide, and can be represented by one or more particular variant sequences, or by a consensus sequence. In another sense, a marker is an isolated variant or consensus of such a sequence. The term also refers to nucleic acid sequences complementary to or flanking the marker sequences, such as nucleic acids used as probes or primer pairs capable of amplifying the marker sequence. A "marker probe" is a nucleic acid sequence or molecule that can be used to identify the presence of a marker locus, e.g., a nucleic acid probe that is complementary to a marker locus sequence. Alternatively, in some aspects, a marker probe refers to a probe of any type that is able to distinguish (i.e., genotype) the particular allele that is present at a marker locus. A "marker locus" is a locus that can be used to track the presence of a second linked locus, e.g., a linked locus that encodes or contributes to expression of a phenotypic trait. For example, a marker locus can be used to monitor segregation of alleles at a locus, such as a QTL, that are genetically or physically linked to the marker locus. Thus, a "marker allele," alternatively an "allele of a marker locus" is one of a plurality of polymorphic nucleotide sequences found at a marker locus in a population that is polymorphic for the marker locus.

[0042] As used herein, "marker assay" means a method for detecting a polymorphism at a particular locus using a particular method. Marker assays thus include, but are not limited to, measurement of at least one phenotype (such as disease resistance, seed color, flower color, or other visually detectable trait as well as any biochemical trait), restriction fragment length polymorphism (RFLP), single base extension, electrophoresis, sequence alignment, allelic specific oligonucleotide hybridization (ASO), random amplified polymorphic DNA (RAPD), microarray-based polymorphism detection technologies, and the like.

[0043] As used herein, "phenotype" means the detectable characteristics of a cell or organism which can be influenced by gene expression.

[0044] As used herein, a "nucleic acid molecule," of naturally occurring origins or otherwise, may be an "isolated" nucleic acid molecule. An isolated nucleic acid molecule is one removed from its native cellular and chromosomal environment. The term "isolated" is not intended to encompass molecules present in their native state. If desired, an isolated nucleic acid may be substantially purified, meaning that it is the predominant species present in a preparation. A substantially purified molecule may be at least about 60% free, preferably at least about 75% free, more preferably at least about 90% free, and most preferably at least about 95% free from the other molecules (exclusive of solvent) present in the preparation.

[0045] As used herein, "quantitative trait locus (QTL)" means a genetic domain that effects a phenotype that can be described in quantitative terms and can be assigned a "phenotypic value" which corresponds to a quantitative value for the phenotypic trait. A QTL can act through a single gene mechanism or by a polygenic mechanism. In some aspects, the invention provides QTL genomic regions, where a QTL (or multiple QTLs) that segregates with low iron tolerance is contained in those regions. In one embodiment of this invention, the boundaries of genomic regions are drawn to encompass markers that will be linked to one or more QTL. In other words, the chromosome interval is drawn such that any marker that lies within that region (including the terminal markers that define the boundaries of the region) is genetically linked to the QTL. Each region comprises at least one QTL, and furthermore, may indeed comprise more than one QTL. Close proximity of multiple QTL in the same region may obfuscate the correlation of a particular marker with a particular QTL, as one marker may demonstrate linkage to more than one QTL. Conversely, e.g., if two markers in close proximity show co-segregation with the desired phenotypic trait, it is sometimes unclear if each of those markers identifying the same QTL or two different QTL. Regardless, knowledge of how many QTL are in a particular interval is not necessary to make or practice the invention.

[0046] As used herein, the term "soybean" means Glycine max and includes all plant varieties that can be bred with soybean, including wild soybean species. In certain embodiments, soybean plants from the species Glycine max and the subspecies Glycine max L. ssp. max or Glycine max ssp. formosana can be genotyped using the compositions and methods of the present invention. In an additional aspect, the soybean plant is from the species Glycine soja, otherwise known as wild soybean, can be genotyped using these compositions and methods. Alternatively, soybean germplasm derived from any of Glycine max, Glycine max L. ssp. max, Glycine max ssp. Formosana, and/or Glycine soja can be genotyped using compositions and methods provided herein.

[0047] As used herein, the term "single nucleotide polymorphism," also referred to by the abbreviation "SNP," means a polymorphism at a single site wherein the polymorphism constitutes any or all of a single base pair change, an insertion of one or more base pairs, and/or a deletion of one or more base pairs.

[0048] As used herein, the phrases "low iron," "low-available iron," "low soluble iron," "low iron conditions," "low iron growth conditions," iron shortage" or "iron deficiency" or the like refer to conditions where iron availability is less than optimal for soybean growth, and can cause plant pathology, e.g., IDC, due to the lack of metabolically-available iron. It is recognized that under "iron deficient" conditions, the absolute concentration of atomic iron may be sufficient, but the form of the iron (e.g., its incorporation into various molecular structures) and other environmental factors may make the iron unavailable for plant use. For example, high carbonate levels, high pH, high salt content, herbicide applications, cool temperatures, saturated soils, or other environmental factors can decrease iron solubility, and reduce the solubilized forms of iron that the plant requires for uptake. One of skill in the art is familiar with assays to measure iron content of soil, as well as those concentrations of iron that are optimal or sub-optimal for plant growth.

[0049] As used herein, the terms "tolerance" or "improved tolerance" in reference to a soybean plant grown in low iron growth conditions is an indication that the soybean plant is less affected by the low-available iron conditions with respect to yield, survivability and/or other relevant agronomic measures, compared to a less tolerant, more "susceptible" plant. Tolerance is a relative term, indicating that a tolerant" plant survives and/or produces better yield of soybean in low-available iron growth conditions compared to a different (less tolerant) plant (e.g., a different soybean strain) grown in similar low-available iron conditions. That is, the low-available iron growth conditions cause a reduced decrease in soybean survival and/or yield in a tolerant soybean plant, as compared to a susceptible soybean plant. As used in the art, iron-deficiency "tolerance" is sometimes used interchangeably with iron-deficiency "resistance."

[0050] One of skill will appreciate that soybean plant tolerance to low-available iron conditions varies widely, and can represent a spectrum of more-tolerant or less-tolerant phenotypes. However, by simple observation, one of skill can generally determine the relative tolerance or susceptibility of different plants, plant lines or plant families under low-available iron conditions, and furthermore, will also recognize the phenotypic gradations of "tolerant."

[0051] In one example, a plant's tolerance can be approximately quantitated using a chlorosis scoring system. In such a system, a plant that is grown in a known iron-deficient area, or in low-available iron experimental conditions, and is assigned a tolerance rating of between 1 (highly susceptible; most or all plants dead; those that live are stunted and have little living tissue) to 9 (highly tolerant; yield and survivability not significantly affected; all plants normal green color). See also, Dahiya and Singh (1979) "Effect of salinity, alkalinity and iron sources on availability of iron," Plant and Soil 51:13-18.

II. Description of the Invention: Overview

[0052] In accordance with the present invention, Applicants have discovered genomic regions, associated markers, and associated methods for identifying and associating genotypes that affect an iron deficient growth condition tolerance trait.

[0053] The advent of molecular genetic markers has facilitated mapping and selection of agriculturally important traits in soybean. Markers tightly linked to tolerance genes are an asset in the rapid identification of tolerant soybean lines on the basis of genotype by the use of marker assisted selection (MAS). Introgressing tolerance genes into a desired cultivar is also facilitated by using suitable nucleic acid markers.

[0054] The use of markers to infer a phenotype of interest results in the economization of a breeding program by substituting costly, time-intensive phenotyping assays with genotyping assays. Further, breeding programs can be designed to explicitly drive the frequency of specific, favorable phenotypes by targeting particular genotypes (U.S. Pat. No. 6,399,855). Fidelity of these associations may be monitored continuously to ensure maintained predictive ability and, thus, informed breeding decisions (U.S. Patent Application 2005/0015827). In this case, costly, time-intensive phenotyping assays required for determining if a plant or plants contains a genomic region associated with a low iron growth condition tolerant phenotype can be supplanted by genotypic assays that provide for identification of a plant or plants that contain the desired genomic region.

III. QTL Associated with Tolerance to Low Iron Growth Conditions

[0055] Provided herewith are certain other QTL that have also been identified as associated with a desirable phenotype of tolerance to growth in low iron conditions when present in certain allelic forms.

[0056] These several soybean QTL provided--that can be associated with a desirable low iron growth condition tolerant phenotype when present in certain allelic forms--are located on soybean chromosome 16 (soybean linkage group J), soybean chromosome 15 (soybean linkage group E), soybean chromosome 7 (soybean linkage group M), soybean chromosome 17 (soybean linkage group D2), and soybean chromosome 10 (soybean linkage group O).

[0057] Tables 1, 3, 5, 7, 9 (corresponding to chromosomes 16, 15, 7, 17, and 10, respectively) shows the relative positions of certain markers that have been disclosed in public databases and non-public (bolded) polymorphic nucleic acid markers, designated SEQ ID NOs, genetic positions (cM) on the chromosome, the allelic forms of certain polymorphic nucleic acid markers associated with a low iron growth condition tolerant phenotype, the allelic forms of those polymorphic nucleic acid markers not associated with the low iron growth condition tolerant phenotype, and the polymorphic position within the sequence of the polymorphic nucleic acid marker. The bolded markers have been identified as within a genomic region associated with a low iron growth condition tolerant phenotype.

[0058] Tables 2, 4, 6, 8, 10 (corresponding to chromosomes 16, 15, 7, 17, and 10, respectively) provides for each polymorphic nucleic acid marker/SEQ ID NO: the linkage group corresponding to the chromosome and the relative physical map positions of the markers.

TABLE-US-00001 TABLE 1 Chromosome 16--QTL on chromosome 16 associated with a low iron growth condition tolerant phenotype. Allelic Allelic cM Form(s) Form(s) Map Associated Not- Position With Associated Marker on Low with Locus Chromo- Fe Low Name/ some Tolerance Fe Poly- SEQ Sixteen [-LOG10 Pheno- Tolerance morphic ID NO. (16) (P)] type¹ Phenotype¹ Position asmbl_10470 * * * * * BI427060 * * * * * 1 104.6 3.2 GG AA 201 2 104.9 3.9 CC GG 201 3 105.2 2.9 GG AA 201 4 105.5 3.9 CC AA 281 5 105.6 3.2 CC TT 618 6 105.6 2.8 CC AA 201 7 105.7 3.0 CC TT 201 8 106.2 2.9 CC TT 201 9 106.3 3.1 GG CC 201 10 106.4 3.8 CC TT 201 11 107.1 3.6 AA CC 201 12 107.2 3.2 GG CC 201 13 107.3 2.7 AA GG 201 14 107.6 3.6 GG AA 201 15 107.6 2.5 CC TT 176 16 107.9 2.5 CC TT 201 17 108.5 2.7 CC TT 201 18 108.8 3.3 TT CC 201 19 108.8 2.6 CC TT 201 20 108.8 3.0 AA CC 925 21 109.5 2.2 TT CC 201 22 109.8 2.4 TT AA 380 23 110.1 2.5 GG AA 201 24 110.5 4.0 TT AA 201 25 110.8 4.3 CC AA 639 26 113.8 3.3 TT AA 123 27 111.1 5.0 CC TT 201 28 111.3 2.5 TT CC 201 29 111.4 2.4 TT CC 201 30 111.5 2.8 CC AA 201 31 111.7 3.7 AA GG 201 32 112 2.8 AA GG 201 33 112.1 2.6 GG CC 201 34 112.3 4.4 AA GG 201 35 112.6 3.3 CC AA 201 36 112.8 3.1 TT CC 347 37 112.9 2.9 TT CC 201 38 113.1 2.8 AA GG 201 39 113.4 2.9 TT GG 201 40 113.6 2.6 TT CC 201 41 113.7 2.7 CC TT 201 42 113.7 2.8 TT CC 155 43 113.7 3.0 AA GG 261 44 113.8 2.4 TT CC 201 45 113.8 2.3 AA GG 194 46 113.9 2.7 GG TT 201 47 114.1 2.4 TT CC 201 48 114.4 2.4 TT CC 285 TA67482_3847 * * * * * TC370075 * * * * *

TABLE-US-00002 TABLE 2 Chromosome 16--Physical positions of certain genetic markers on soybean chromosome 16 in proximity to QTL associated with a low iron growth condition tolerant phenotype. Marker Locus Name/SEQ ID Linkage Chromo- Middle Start End NO. Group some Position Position Position asmbl_10470 J 16 31363336 31362972 31363701 BI427060 J 16 31363515 31363238 31363792 1 J 16 31369584 31369434 31369735 2 J 16 31443226 31443076 31443377 3 J 16 31477826 31478015 31477638 4 J 16 31480456 31480836 31480077 5 J 16 31486792 31486642 31486943 6 J 16 31480983 31480833 31481134 7 J 16 31510525 31510375 31510676 8 J 16 31516209 31516059 31516360 9 J 16 31521798 31521648 31521949 10 J 16 31553067 31552917 31553218 11 J 16 31557341 31557191 31557492 12 J 16 31563379 31563229 31563530 13 J 16 31574585 31574435 31574736 14 J 16 31575341 31575137 31575545 15 J 16 31577004 31576854 31577155 16 J 16 31580785 31580635 31580936 17 J 16 31614520 31614370 31614671 18 J 16 31614870 31615435 31614305 19 J 16 31618039 31617889 31618190 20 J 16 31643780 31643630 31643931 21 J 16 31785190 31785040 31785341 22 J 16 31804506 31804356 31804657 23 J 16 31831923 31831773 31832074 24 J 16 31889649 31889499 31889800 25 J 16 31904942 31904792 31905093 26 J 16 31911271 31911121 31911422 27 J 16 31925976 31925826 31926127 28 J 16 31952216 31952066 31952367 29 J 16 31990398 31990248 31990549 30 J 16 31997204 31997054 31997355 31 J 16 32031978 32031828 32032129 32 J 16 32079262 32079112 32079413 33 J 16 32100628 32100039 32101218 34 J 16 32154510 32154694 32154327 35 J 16 32161907 32161757 32162058 36 J 16 32204175 32204025 32204326 37 J 16 32242461 32242311 32242612 38 J 16 32279082 32278743 32279421 39 J 16 32291528 32291900 32291157 40 J 16 32347306 32347156 32347457 41 J 16 32434829 32446198 32423461 42 J 16 32484745 32484595 32484896 43 J 16 32519227 32519077 32519378 44 J 16 32672805 32672655 32672956 45 J 16 32852516 32852366 32852667 46 J 16 32852826 32852442 32853211 47 J 16 32854325 32854591 32854059 48 J 16 31374689 31374539 31374840 TA67482_3847 J 16 32859166 32858544 32859789 TC370075 J 16 32859832 32859787 32859877

TABLE-US-00003 TABLE 3 Chromosome 15--QTL on chromosome 15 associated with a low iron growth condition tolerant phenotype. Allelic cM Allelic Form(s) Map Form(s) Not- Position Associated Associated on With with Marker Chromo- Low Low Locus some Fe Fe Poly- Name/ Fifteen [-LOG Tolerance Tolerance morphic SEQ ID NO (15) 10(P)] Phenotype¹ Phenotype¹ Position DB975811 * * * * * TA67841_3847 * * * * * 49 22.5 3.0 TT CC 201 50 22.7 4.1 CC TT 201 51 22.9 3.1 CC GG 201 52 23.6 3.4 TT CC 201 53 23.7 3.6 CC TT 201 54 23.9 3.4 TT GG 354 55 23.9 4.1 CC CC 355 56 24.1 3.2 TT AA 61 57 24.7 4.1 AA GG 201 58 24.7 4.1 GG AA 240 59 24.7 4.1 GG AA 428 60 24.9 3.3 CC TT 993 61 25.4 3.9 TT CC 201 62 25.6 5.6 TT CC 201 63 26.4 3.9 CC AA 201 64 31.4 3.0 TT CC 201 65 31.6 3.0 TT GG 201 66 31.7 3.1 AA GG 201 67 31.8 4.6 GG AA 201 68 32.4 3.1 AA GG 201 69 32.5 3.3 GG AA 201 TC370174 * * * * * Glyma15g06010 * * * * *

TABLE-US-00004 TABLE 4 Chromosome 15--Physical positions of certain genetic markers on soybean chromosome 5 in proximity to QTL associated with a low iron growth condition tolerant phenotype. Marker Locus Linkage Chromo- Middle Start End Name/SEQ ID NO. Group some Position Position Position DB975811 E 15 2656905 2656345 2657466 TA67841_3847 E 15 2656990 2656430 2657551 49 E 15 2657551 2657401 2657702 50 E 15 2674098 2673948 2674249 51 E 15 2701610 2701460 2701761 52 E 15 2796176 2796026 2796327 53 E 15 2822719 2822569 2822870 54 E 15 2847164 2847410 2846919 55 E 15 2847207 2847057 2847358 56 E 15 3039453 3039024 3039882 57 E 15 3043572 3043814 3043331 58 E 15 3048934 3048784 3049085 59 E 15 3154544 3154394 3154695 60 E 15 3178097 3177947 3178248 61 E 15 3301037 3300887 3301188 62 E 15 3402856 3402706 3403007 63 E 15 3756773 3756509 3757037 64 E 15 2662233 2662435 2662031 65 E 15 2663725 2663953 2663497 66 E 15 2667350 2667200 2667501 67 E 15 2697692 2697542 2697843 68 E 15 2722948 2722798 2723099 69 E 15 2729212 2729062 2729363 TC370174 E 15 2772449 2772299 2772600 Glyma15g06010 E 15 2790560 2790410 2790711

TABLE-US-00005 TABLE 5 Chromosome 7--QTL on chromosome 7 associated with a low iron growth condition tolerant phenotype. Allelic cM Allelic Form(s) Map Form(s) Not- Position Associated Associated on With Low with Marker Chromo Fe Low Locus some Tolerance Fe Poly- Name/ Seven [-LOG10 Pheno- Tolerance morphic SEQ ID NO. (7) (P)] type¹ Phenotype¹ Position TA75172_3847 * * * * * Contig4349 * * * * * 70 46.7 1.5 AA GG 201 71 51.8 1.5 AA GG 650 72 53.5 1.9 AA TT 201 73 53.7 2.0 TT GG 201 74 54.5 1.8 GG TT 201 75 58.6 1.4 GG AA 201 76 58.9 1.5 GG AA 201 77 59.6 1.4 TT CC 201 78 59.7 2.4 GG AA 201 79 59.8 2.1 TT CC 201 80 96.4 3.0 TT CC 201 81 98.2 3.0 GG CC 201 82 98.6 3.2 TT CC 201 83 102.1 2.3 GG AA 201 84 102.8 2.2 AA GG 201 85 102.9 2.2 AA GG 201 AW705305 * * * * * TC380682 * * * * *

TABLE-US-00006 TABLE 6 Chromosome 7--Physical positions of certain genetic markers on soybean chromosome 7 in proximity to QTL associated with a low iron growth condition tolerant phenotype. Marker Locus Name/ Linkage Chromo- Middle Start End SEQ ID NO. Group some Position Position Position TA75172_3847 M 7 5421539 5421375 5421704 Contig4349 M 7 5422610 5422460 5422761 70 M 7 6777792 6777642 6777943 71 M 7 6814309 6814159 6814460 72 M 7 6911064 6910914 6911215 73 M 7 7602143 7601993 7602294 74 M 7 7671547 7671397 7671698 75 M 7 7801323 7801173 7801474 76 M 7 7821649 7821499 7821800 77 M 7 7829639 7829489 7829790 78 M 7 17390045 17389895 17390196 79 M 7 17724916 17724766 17725067 80 M 7 17812664 17812514 17812815 81 M 7 18464522 18464372 18464673 82 M 7 18592542 18592392 18592693 83 M 7 18594332 18594182 18594483 84 M 7 18592542 18592392 18592693 85 M 7 18594332 18594182 18594483 AW705305 M 7 18594647 18594463 18594831 TC380682 M 7 18594649 18594451 18594848

TABLE-US-00007 TABLE 7 Chromosome 17--QTL on chromosome 17 associated with a low iron growth condition tolerant phenotype. Allelic Allelic cM Form(s) Form(s) Map Associated Not- Position With Associated Marker on Low with Locus Chromo Fe Low Fe Name/ some Tolerance Tolerance Poly- SEQ ID Seven- [-LOG10 Pheno- Pheno- morphic NO teen (17) (P)] type¹ type¹ Position TC350035 * * * * * TA43074_3847 * * * * * 86 5.2 2.0 GG TT 201 87 5.4 2.0 TT GG 201 88 5.6 3.2 CC TT 201 89 5.7 3.3 AA TT 201 90 5.8 3.2 TT GG 201 91 5.8 3.3 AA GG 201 92 5.9 3.1 GG CC 201 93 6 3.2 TT GG 201 94 6.1 3.1 GG AA 201 95 6.3 3.1 AA CC 201 96 6.5 3.1 AA TT 201 97 6.6 3.0 TT AA 201 98 6.8 3.0 GG AA 201 99 7.1 3.0 GG CC 439 100 7.3 3.0 CC TT 201 101 7.5 2.4 CC GG 201 102 7.7 2.4 CC AA 201 103 8 3.2 AA TT 201 104 8.1 3.0 GG AA 201 105 8.2 2.8 TT CC 201 106 8.2 2.7 GG AA 201 107 8.4 2.7 TT CC 201 108 8.5 2.8 CC TT 201 109 8.8 3.0 TT CC 201 110 9 2.8 AA CC 201 111 9.2 3.5 TT GG 201 112 9.3 3.6 TT AA 201 Glyma17g01380 * * * * * Gm_W82_ * * * * * CR17.G8870

TABLE-US-00008 TABLE 8 Chromosome 17--Physical positions of certain genetic markers on soybean chromosome 17 in proximity to QTL associated with a low iron growth condition tolerant phenotype. Marker Locus Name/ Linkage Chromo- Middle Start End SEQ ID NO Group some Position Position Position TC350035 D2 17 280819 279005 282633 TA43074_3847 D2 17 280856 279080 282633 86 D2 17 282695 282545 282846 87 D2 17 317160 317010 317311 88 D2 17 342956 342806 343107 89 D2 17 349222 349072 349373 90 D2 17 358930 358780 359081 91 D2 17 368357 368207 368508 92 D2 17 384568 384418 384719 93 D2 17 397053 396903 397204 94 D2 17 408927 408777 409078 95 D2 17 435118 434968 435269 96 D2 17 464163 464013 464314 97 D2 17 479395 479245 479546 98 D2 17 495991 495841 496142 99 D2 17 545090 544843 545337 100 D2 17 574773 574623 574924 101 D2 17 602079 601929 602230 102 D2 17 618344 618194 618495 103 D2 17 670615 670465 670766 104 D2 17 683816 683666 683967 105 D2 17 685126 684976 685277 106 D2 17 693456 693306 693607 107 D2 17 717263 717113 717414 108 D2 17 732375 732225 732526 109 D2 17 782062 781912 782213 110 D2 17 809659 809509 809810 111 D2 17 823632 823482 823783 112 D2 17 840172 840022 840323 Glyma17g01380 D2 17 844200 842706 845694 Gm_W82_CR17.G8870 D2 17 844200 842706 845694

TABLE-US-00009 TABLE 9 Chromosome 10--QTL on chromosome 10 associated with a low iron growth condition tolerant phenotype. Allelic Allelic cM Form(s) Form(s) Map Asso- Not-Asso- Position ciated ciated on With with Poly- Chromo- Low Fe Low Fe mor- some Tolerance Tolerance phic Marker Locus Seven- [-LOG10 Pheno- Pheno- Posi- Name/SEQ ID NO teen (10) (P)] type¹ type¹ tion NA * * * * * WmFPC_Contig227 * * * * * 113 85.9 2.4 TT CC 201 114 86.3 2.7 CC TT 201 115 86.6 2.5 AA GG 201 116 86.7 2.6 CC TT 201 117 86.8 2.3 CC GG 201 118 86.9 2.6 GG AA 201 119 87.2 2.5 CC TT 201 120 87.3 2.7 TT AA 201 121 87.4 2.4 GG AA 201 122 87.5 2.2 CC TT 201 123 87.6 2.8 CC TT 201 124 87.7 2.9 CC TT 201 125 87.8 2.7 GG AA 136 126 88 2.5 TT GG 201 127 88.1 2.6 CC TT 219 128 89.7 2.5 AA GG 201 129 89.8 2.5 GG AA 201 130 90.1 2.7 AA TT 201 131 90.9 2.8 TT CC 201 132 91.2 3.7 AA GG 201 133 91.3 2.8 AA CC 201 134 91.4 2.1 GG CC 201 135 91.5 3.9 AA GG 201 136 91.7 2.2 TT GG 201 137 91.9 3.9 TT AA 201 138 92 3.7 GG AA 201 139 92.1 3.7 CC AA 201 140 92.2 3.7 GG AA 201 141 92.4 3.9 TT CC 201 142 93.9 2.1 GG CC 201 143 94.3 2.1 GG AA 201 144 94.4 2.1 TT CC 201 145 94.5 2.1 CC AA 201 146 95.8 2.4 TT AA 201 147 101 2.1 CC TT 201 Glyma10g28920 * * * * * Cf16144d * * * * *

TABLE-US-00010 TABLE 10 Chromosome 10--Physical positions of certain genetic markers on soybean chromosome 10 in proximity to QTL associated with a low iron growth condition tolerant phenotype. Marker Locus Link- Name/ age Chromo- Middle Start End SEQ ID NO Group some Position Position Position NA O 10 29358961 11677282 47040641 WmFPC_Contig227 O 10 29398139 28618646 30177632 113 O 10 29720865 29720715 29721016 114 O 10 30158032 30157882 30158183 115 O 10 30164795 30164645 30164946 116 O 10 30168646 30168496 30168797 117 O 10 30173472 30173322 30173623 118 O 10 30197793 30197643 30197944 119 O 10 30250601 30250451 30250752 120 O 10 30265182 30265032 30265333 121 O 10 30296454 30296304 30296605 122 O 10 30302927 30302777 30303078 123 O 10 30338781 30338631 30338932 124 O 10 30339883 30339733 30340034 125 O 10 30368472 30368234 30368710 126 O 10 30785142 30784992 30785293 127 O 10 30923459 30923014 30923904 128 O 10 36092106 36091956 36092257 129 O 10 36297861 36297711 36298012 130 O 10 37237431 37237281 37237582 131 O 10 37349903 37349753 37350054 132 O 10 37364859 37364709 37365010 133 O 10 37381677 37381527 37381828 134 O 10 37396158 37396008 37396309 135 O 10 37396896 37396746 37397047 136 O 10 37428843 37428693 37428994 137 O 10 37453860 37453710 37454011 138 O 10 37465158 37465008 37465309 139 O 10 37479303 37479153 37479454 140 O 10 37492059 37491909 37492210 141 O 10 37525000 37524850 37525151 142 O 10 37718944 37718794 37719095 143 O 10 37753981 37753831 37754132 144 O 10 37761477 37761327 37761628 145 O 10 37763485 37763284 37763687 146 O 10 37898074 37897924 37898225 147 O 10 38394420 38394270 38394571 Glyma10g28920 O 10 38395822 38395602 38396043 Cf16144d O 10 38397079 38396831 38397328

IV. Identification of Plants Exhibiting Tolerance to Low Iron Growth Conditions

[0059] To observe the presence or absence of low iron growth condition tolerant phenotypes, soybean plants comprising genotypes of interest can be exposed to low iron or iron deficient growth conditions in seedling stages, early to mid-vegetative growth stages, or in early reproductive stages. Experienced plant breeders can recognize tolerant soybean plants in the field, and can select the tolerant individuals or populations for breeding purposes or for propagation. In this context, the plant breeder recognizes "tolerant" and "susceptible" soybean plants in fortuitous naturally-occurring filed observations.

[0060] Breeders will appreciate that plant tolerance is a phenotypic spectrum consisting of extremes in tolerance, susceptibility, and a continuum of intermediate phenotypes. Tolerance also varies due to environmental effects. Evaluation of phenotypes using reproducible assays and tolerance scoring methods are of value to scientists who seek to identify genetic loci that impart tolerance, conduct marker assisted selection to create tolerant soybean populations, and for introgression techniques to breed a tolerance trait into an elite soybean line, for example.

[0061] In contrast to fortuitous field observations that classify plants as either "tolerant" or "susceptible," various methods are known in the art for determining (and quantitating) the tolerance of a soybean plant to iron-deficient growth conditions. These techniques can be applied to different fields at different times, or to experimental greenhouse or laboratory settings, and provide approximate tolerance scores that can be used to characterize the tolerance of a given strain or line regardless of growth conditions or location. See, for example, Diers et al. (1992) "Possible identification of quantitative trait loci affecting iron efficiency in soybean," J. Plant Nutr. 15:217-2136; Dahiya and M. Singh (1979) "Effect of salinity, alkalinity and iron sources on availability of iron," Plant and Soil 51:13-18; and Gonzalez-Vallejo et al. (2000) "Iron Deficiency Decreases the Fe(III)-Chelate Reducing Activity of Leaf Protoplasts" Plant Physiol. 122 (2): 337-344.

[0062] The degree of IDC in a particular plant or stand of plants can be quantitated by using a system to score the severity of the disease in each plant. A plant strain or variety or a number of plant strains or varieties are planted and grown in a single stand in soil that is known to produce chlorotic plants as a result of iron deficiency ("field screens," i.e., in fields that have previously demonstrated IDC), or alternatively, in controlled nursery hydroponic conditions. When the assay is conducted in controlled nursery conditions, defined soils can be used, where the concentration of iron (e.g., available iron) has been previously measured. The plants can be scored at maturity, or at any time before maturity.

[0063] Fifteen (15) to twenty (20) soybean plants are planted and grown in a greenhouse. After a ten (10) day period, the plants are moved to a growth chamber. The growth chamber is kept at 25° C. day, 22° C. night with a relative humidity of 60% and light intensity of 200-500 microeinsteins and under a 16 hr photo-period. Water (3.5 gallons) plus the IDC nutrient solution is added to each test box. Water (3.5 gallons) plus the IDC nutrient solution and iron is added to the control box. Once boxes are filled with water and the solution, the pH is measured and adjusted to a range of 7.8-8.0. Nine (9) of the 15-20 plants are selected from each line. Two 3-plant groupings will be placed in two different boxes and the third grouping will be placed in the control box. Plants are kept in the growth chamber for a period of five (5) days. During that time, pH is measured and adjusted as necessary. At day five (5), all three (3) plants are evaluated as a group with a phenotypic score of 1-5. Plants receive a score of 1 if their leaves remain green, show no yellowing, and are comparable to the control within the control box. Additional scores will be given from a range of 1 (no yellowing) to 5 (severe yellowing) compared to their internal check lines. Nursey hydroponic conditions are normalized (1-9 scale) to correspond with disease ratings of soybean plants in field conditions.

[0064] The scoring system rates each plant on a scale of one (1) (most tolerant-no disease) to nine (9) (most susceptible-most severe disease), as shown in Table 11.

TABLE-US-00011 TABLE 11 IDC Score Ratings Plant or Plant Stand Score Symptoms 9 Most plants are completely dead. The plants that are still alive are approximately 10% of normal height, and have very little living tissue. 8 Most leaves are almost dead, most stems are still green. Plants are severely stunted (10-20% of normal height). 7 Most plants are yellow and necrosis is seen on most leaves. Most plants are approximately 20-40% of normal height. 6 Most plants are yellow, and necrosis is seen on the edges of less than half the leaves. Most plants are approximately 50% of normal height. 5 Most plants are light green to yellow, and no necrosis is seen on the leaves. Most plants are stunted (50-75% of normal height). 4 More than half the plants show moderate chlorosis, but no necrosis is seen on the leaves. 3 Less than half of the plants showing moderate chlorosis (light green leaves). 2 A few plants are showing very light chlorosis on one or two leaves. 1 All plants are normal green color.

[0065] It will be appreciated that any such scale is relative, and furthermore, there may be variability between practitioners as to how the individual plants and the entire stand as a whole are scored. Optionally, the degree of chlorosis can be measured using a chlorophyll meter, e.g., a Minolta SPAD-502 Chlorophyll Meter, where readings off a single plant or a stand of plants can be made. Optionally, multiple readings can be obtained and averaged.

[0066] In general, while there is a certain amount of subjectivity to assigning severity measurements for disease symptoms, assignment to a given scale as noted above is well within the ordinary skill of a practitioner in the field. Measurements can also be averaged across multiple scores to reduce variation in field measurements.

V. Introgression of a Genomic Region Associated with a Low Iron Growth Condition Tolerance Phenotype

[0067] Provided herewith are unique soybean germplasms comprising one or more introgressed genomic regions, QTL, or polymorphic nucleic acid markers associated with a low iron growth condition tolerant phenotype and methods of obtaining the same. Marker-assisted introgression involves the transfer of a chromosomal region, defined by one or more markers, from one germplasm to a second germplasm. Offspring of a cross that contain the introgressed genomic region can be identified by the combination of markers characteristic of the desired introgressed genomic locus from a first germplasm (e.g., a low iron growth condition tolerant germplasm) and both linked and unlinked markers characteristic of the desired genetic background of a second germplasm (e.g., a low iron growth condition susceptible germplasm). In addition to the polymorphic nucleic acid markers provided herewith that identify alleles of certain QTL associated with a low iron growth condition tolerant phenotype, flanking markers that fall on both the telomere proximal end and the centromere proximal end of the genomic intervals comprising the QTL are also provided in Tables 1-10. Such flanking markers are useful in a variety of breeding efforts that include, but are not limited to, introgression of genomic regions associated with a low iron growth condition tolerant phenotype into a genetic background comprising markers associated with germplasm that ordinarily contains a genotype associated with a susceptible phenotype and maintenance of those genomic regions associated with a low iron growth condition tolerant phenotype in a plant population. Numerous markers that are linked and either immediately adjacent or adjacent to a low iron growth condition tolerant QTL in soybean that permit introgression of low iron growth condition tolerant QTL in the absence of extraneous linked DNA from the source germplasm containing the QTL are provided herewith. In certain embodiments, the linked and immediately adjacent markers are within about 105 kilobases (kB), 80 kB, 60 kB, 50 kB, 40 kB, 30 kB, 20 kB, 10 kB, 5 kB, 1 kB, 0.5 kB, 0.2 kB, or 0.1 kB of the introgressed genomic region. In certain embodiments, the linked and adjacent markers are within 1,000 kB, 600 kB, 500 kB, 400 kB, 300 kB, 200 kB, or 150 kB of the introgressed genomic region. In certain embodiments, the linked markers are genetically linked within about 50 cM, 40 cM, 30 cM, 20 cM, 10 cM, 5 cM, 4 cM, 3 cM, 2 cM, or 1 cM of the introgressed genomic region. In certain embodiments, genomic regions comprising some or all of one or more of a low iron growth condition tolerant QTL described herein can be introgressed into the genomes of susceptible varieties by using markers that include, but are not limited to, genetically linked markers, adjacent markers, and/or immediately adjacent markers provided in Tables 1-10. Those skilled in the art will appreciate that when seeking to introgress a smaller genomic region comprising a low iron growth condition tolerant QTL locus described herein, that any of the telomere proximal or centromere proximal markers that are genetically linked to or immediately adjacent to a larger genomic region comprising a low iron growth condition tolerant QTL locus can also be used to introgress that smaller genomic region.

[0068] Provided herein are methods of introgressing any of the genomic regions comprising a low iron growth condition tolerance QTL locus of Tables 1-10 into soybean germplasm that lacks such a locus. In certain embodiments, the soybean germplasm that lacks such a genomic region comprising a low iron growth condition tolerance QTL locus of Tables 1-10 is susceptible or has less than optimal levels of tolerance to low iron growth conditions. In certain embodiments, the methods of introgression provided herein can yield soybean plants comprising introgressed genomic regions comprising one or more low iron growth condition tolerance QTL loci of Tables 1-10, where the immediately adjacent genomic DNA and/or some or all of the adjacent genomic DNA between the introgressed genomic region and the telomere or centromere will comprise allelic forms of the markers of Tables 1-11 that are characteristic of the germplasm into which the genomic region is introgressed and distinct from the germplasm from which the genomic region is derived. In certain embodiments, the soybean germplasm into which the genomic region is introgressed is germplasm that lacks such a low iron growth condition tolerance locus. In certain embodiments, the soybean germplasm into which the genomic region is introgressed is germplasm that lacks such a low iron growth condition tolerance locus and is either susceptible to low iron growth conditions or has less than optimal tolerance to low iron growth conditions.

[0069] Also provided herein are soybean plants produced by the aforementioned methods of introgression. In certain embodiments, the soybean plants will comprise introgressed genomic regions comprising a low iron growth condition tolerance QTL locus of Tables 1-10, where the immediately adjacent genomic DNA and/or some or all of the adjacent genomic DNA between the introgressed genomic region and the telomere or centromere will comprise allelic forms of the markers of Tables 1-10 that are characteristic of the germplasm into which the genomic region is introgressed and distinct from the germplasm from which the genomic region is derived.

[0070] Soybean plants or germplasm comprising an introgressed genomic region that is associated with a low iron growth condition tolerant phenotype, wherein at least 10%, 25%, 50%, 75%, 90%, or 99% of the remaining genomic sequences carry markers characteristic of soybean plants or germplasm that are otherwise or ordinarily comprise a genomic region associated with susceptibility to low iron growth conditions, are thus provided. Furthermore soybean plants comprising an introgressed region where closely linked regions adjacent and/or immediately adjacent to the genomic regions, QTL, and markers provided herewith that comprise genomic sequences carrying markers characteristic of soybean plants or germplasm that are otherwise or ordinarily comprise a genomic region associated with the susceptibility to low iron growth conditions are also provided.

[0071] Also provided herein are methods of creating a population of soybean plants with enhanced tolerance to low iron growth conditions. In certain embodiments, the maintenance of a low iron growth condition tolerance QTL locus is achieved by providing a population of soybean plants, detecting the presence of a genetic marker that is genetically linked to the QTL, selecting one or more soybean plants containing said marker from the first population of soybean plants, and producing a population of offspring from the at least one selected soybean plants. In certain embodiments, the tolerance QTL are selected from Tables 1-10. In certain embodiments, the markers are genetically linked to the QTL in Tables 1-10. In certain embodiments, the markers are genetically linked to the tolerance QTL within 20 cM, 15 cM, 10 cM, 5 cM, 4 cM, or 3 cM. In certain embodiments, the genetic markers are selected from SEQ ID NOs. 1-147.

VI. Soybean Donor Plants Comprising Genomic Region Associated with Low Iron Growth Condition Phenotypes

[0072] Low iron growth condition tolerance QTL allele or alleles can be introduced from any plant that contains that allele (donor) to any recipient soybean plant. In one aspect, the recipient soybean plant can contain additional low iron growth condition tolerance loci. In another aspect, the recipient soybean plant can contain a transgene. In another aspect, while maintaining the introduced QTL, the genetic contribution of the plant providing the low iron growth condition tolerance QTL can be reduced by back-crossing or other suitable approaches. In one aspect, the nuclear genetic material derived from the donor material in the soybean plant can be less than or about 50%, less than or about 25%, less than or about 13%, less than or about 5%, 3%, 2% or 1%, but that genetic material contains the low iron growth condition tolerance locus or loci of interest.

[0073] Plants containing one or more of the low iron growth condition tolerance loci described herein can be donor plants. In certain embodiments, a donor plant can be a susceptible line. In certain embodiments, a donor plant can also be a recipient soybean plant. A non-limiting and exemplary list of soybean varieties that are believed to comprise genomic regions associated with a low iron growth condition tolerance phenotype include, but are not limited to AG00501, AG00901, AG0131, AG0202, AG0231, AG0331, AG0401, AG801, AG0808, AG1031, AG1102, AG1230, AG2131, DKB22-52, AG3039, and AG3830 (Branded names of ASGROW (designated "AG") and DEKALB soybean varieties from Monsanto CO., 800 N. Lindbergh Blvd., St. Louis, Mo., USA.)

[0074] In a preferred embodiment, the soybean plants that comprise a genomic region associated with a low iron growth condition tolerance phenotype that are identified by use of the markers provided in Tables 1-10 and/or methods provided herein are soybean pre-commercial lines evaluated in an association study using a linear model and which is adjusted for stratification by principle components in the R GenABEL package.

[0075] In certain embodiment, a donor soybean plant is AG801 and derivatives thereof, and is used as the resistant parent in a bi-parental mapping population to select for genomic regions associated with a low iron growth condition tolerance phenotype.

[0076] Also provided herewith are additional soybean plants that comprise a genomic region associated with a low iron growth condition tolerance phenotype that are identified by use of the markers provided in Tables 1-11 and/or methods provided herein. Any of the soybean plants identified above or other soybean plants that are otherwise identified using the markers or methods provided herein can be used in methods that include, but are not limited to, methods of obtaining soybean plants with an introgressed low iron growth condition tolerance locus, obtaining a soybean plant that exhibits a low iron growth condition tolerance phenotype, or obtaining a soybean plant comprising in its genome a genetic region associated with a low iron growth condition tolerance phenotype.

[0077] In certain embodiments, the soybean plants provided herein or used in the methods provided herein can comprise a transgene that confers tolerance to glyphosate. Transgenes that can confer tolerance to glyphosate include, but are not limited to, transgenes that encode glyphosate tolerant Class I EPSPS (5-enolpyruvylshikimate-3-phosphate synthases) enzymes or glyphosate tolerant Class II EPSPS (5-enolpyruvylshikimate-3-phosphate synthases) enzymes. Useful glyphosate tolerant EPSPS enzymes provided herein are disclosed in U.S. Pat. No. 6,803,501, RE39,247, U.S. Pat. No. 6,225,114, U.S. Pat. No. 5,188,642, and U.S. Pat. No. 4,971,908. In certain embodiments, the glyphosate tolerant soybean plants can comprise a transgene encoding a glyphosate oxidoreductase or other enzyme which degrades glyphosate. Glyphosate oxidoreductase enzymes had been described in U.S. Pat. No. 5,776,760 and U.S. Reissue Pat. No. RE38,825. In certain embodiments the soybean plant can comprise a transgene encoding a glyphosate N-acetyltransferase gene that confers tolerance to glyphosate. In certain embodiments, the soybean plant can comprise a glyphosate n-acetyltransferase encoding transgene such as those described in U.S. Pat. No. 7,666,644. In still other embodiments, soybean plants comprising combinations of transgenes that confer glyphosate tolerance are provided. Soybean plants comprising both a glyphosate resistant EPSPS and a glyphosate N-acetyltransferase are also provided herewith. In certain embodiments, it is contemplated that the soybean plants used herein can comprise one or more specific genomic insertion(s) of a glyphosate tolerant transgene including, but not limited to, as those found in: i) MON89788 soybean (deposited under ATCC accession number PTA-6708 and described in US Patent Application Publication Number 20100099859), ii) GTS 40-3-2 soybean (Padgette et al., Crop Sci. 35: 1451-1461, 1995), iii) event 3560.4.3.5 soybean (seed deposited under ATCC accession number PTA-8287 and described in U.S. Patent Publication 20090036308), or any combination of i (MON89788 soybean), ii (GTS 40-3-2 soybean), and iii (event 3560.4.3.5 soybean).

[0078] A low iron growth condition tolerance associated QTL of the present invention may also be introduced into a soybean line comprising one or more transgenes that confer tolerance to herbicides including, but not limited to, glufosinate, dicamba, chlorsulfuron, and the like, increased yield, insect control, fungal disease resistance, virus resistance, nematode resistance, bacterial disease resistance, mycoplasma disease resistance, modified oils production, high oil production, high protein production, germination and seedling growth control, enhanced animal and human nutrition, low raffinose, environmental stress resistant, increased digestibility, industrial enzymes, pharmaceutical proteins, peptides and small molecules, improved processing traits, improved flavor, nitrogen fixation, hybrid seed production, reduced allergenicity, biopolymers, and biofuels among others. These agronomic traits can be provided by the methods of plant biotechnology as transgenes in soybean.

[0079] In certain embodiments, it is contemplated that genotypic assays that provide for non-destructive identification of the plant or plants can be performed either in seed, the emergence stage, the "VC" stage (i.e. cotyledons unfolded), the V1 stage (appearance of first node and unifoliate leaves), the V2 stage (appearance of the first trifoliate leaf), and thereafter. In certain embodiments, non-destructive genotypic assays are performed in seed using apparati and associated methods as described in U.S. Pat. Nos. 6,959,617; 7,134,351; 7,454,989; 7,502,113; 7,591,101; 7,611,842; and 7,685,768, which are incorporated herein by reference in their entireties. In certain embodiments, non-destructive genotypic assays are performed in seed using apparati and associated methods as described in U.S. Patent Application Publications 20100086963, 20090215060, and 20090025288, which are incorporated herein by reference in their entireties. Published U.S. Patent Applications US 2006/0042527, US 2006/0046244, US 2006/0046264, US 2006/0048247, US 2006/0048248, US 2007/0204366, and US 2007/0207485, which are incorporated herein by reference in their entirety, also disclose apparatus and systems for the automated sampling of seeds as well as methods of sampling, testing and bulking seeds. Thus, in certain embodiments, any of the methods provided herein can comprise screening for markers in individual seeds of a population wherein only seed with at least one genotype of interest is advanced.

VII. Molecular Assisted Breeding Techniques

[0080] Genetic markers that can be used in the practice of the instant invention include, but are not limited to, are Restriction Fragment Length Polymorphisms (RFLP), Amplified Fragment Length Polymorphisms (AFLP), Simple Sequence Repeats (SSR), Single Nucleotide Polymorphisms (SNP), Insertion/Deletion Polymorphisms (Indels), Variable Number Tandem Repeats (VNTR), and Random Amplified Polymorphic DNA (RAPD), and others known to those skilled in the art. Marker discovery and development in crops provides the initial framework for applications to marker-assisted breeding activities (U.S. Patent Applications 2005/0204780, 2005/0216545, 2005/0218305, and 2006/00504538). The resulting "genetic map" is the representation of the relative position of characterized loci (DNA markers or any other locus for which alleles can be identified) along the chromosomes. The measure of distance on this map is relative to the frequency of crossover events between sister chromatids at meiosis.

[0081] As a set, polymorphic markers serve as a useful tool for fingerprinting plants to inform the degree of identity of lines or varieties (U.S. Pat. No. 6,207,367). These markers can form a basis for determining associations with phenotype and can be used to drive genetic gain. The implementation of marker-assisted selection is dependent on the ability to detect underlying genetic differences between individuals.

[0082] Certain genetic markers for use in the present invention include "dominant" or "codominant" markers. "Codominant markers" reveal the presence of two or more alleles (two per diploid individual). "Dominant markers" reveal the presence of only a single allele. The presence of the dominant marker phenotype (e.g., a band of DNA) is an indication that one allele is present in either the homozygous or heterozygous condition. The absence of the dominant marker phenotype (e.g., absence of a DNA band) is merely evidence that "some other" undefined allele is present. In the case of populations where individuals are predominantly homozygous and loci are predominantly dimorphic, dominant and codominant markers can be equally valuable. As populations become more heterozygous and multiallelic, codominant markers often become more informative of the genotype than dominant markers.

[0083] In another embodiment, markers that include. but are not limited, to single sequence repeat markers (SSR), AFLP markers, RFLP markers, RAPD markers, phenotypic markers, isozyme markers, single nucleotide polymorphisms (SNPs), insertions or deletions (Indels), single feature polymorphisms (SFPs, for example, as described in Borevitz et al. 2003 Gen. Res. 13:513-523), microarray transcription profiles, DNA-derived sequences, and RNA-derived sequences that are genetically linked to or correlated with low iron growth condition tolerance loci, regions flanking low iron growth condition tolerance loci, regions linked to low iron growth condition tolerance loci, and/or regions that are unlinked to low iron growth condition tolerance loci can be used in certain embodiments of the instant invention.

[0084] In one embodiment, nucleic acid-based analyses for determining the presence or absence of the genetic polymorphism (i.e. for genotyping) can be used for the selection of seeds in a breeding population. A wide variety of genetic markers for the analysis of genetic polymorphisms are available and known to those of skill in the art. The analysis may be used to select for genes, portions of genes, QTL, alleles, or genomic regions (Genotypes) that comprise or are linked to a genetic marker that is linked to or correlated with low iron growth condition tolerance loci, regions flanking low iron growth condition tolerance loci, regions linked to low iron growth condition tolerance loci, and/or regions that are unlinked to low iron growth condition tolerance loci can be used in certain embodiments of the instant invention.

[0085] Herein, nucleic acid analysis methods include, but are not limited to, PCR-based detection methods (for example, TaqMan assays), microarray methods, mass spectrometry-based methods and/or nucleic acid sequencing methods. In one embodiment, the detection of polymorphic sites in a sample of DNA, RNA, or cDNA may be facilitated through the use of nucleic acid amplification methods. Such methods specifically increase the concentration of polynucleotides that span the polymorphic site, or include that site and sequences located either distal or proximal to it. Such amplified molecules can be readily detected by gel electrophoresis, fluorescence detection methods, or other means.

[0086] A method of achieving such amplification employs the polymerase chain reaction (PCR) (Mullis et al. 1986 Cold Spring Harbor Symp. Quant. Biol. 51:263-273; European Patent 50,424; European Patent 84,796; European Patent 258,017; European Patent 237,362; European Patent 201,184; U.S. Pat. No. 4,683,202; U.S. Pat. No. 4,582,788; and U.S. Pat. No. 4,683,194), using primer pairs that are capable of hybridizing to the proximal sequences that define a polymorphism in its double-stranded form.

[0087] Methods for typing DNA based on mass spectrometry can also be used. Such methods are disclosed in U.S. Pat. Nos. 6,613,509 and 6,503,710, and references found therein.

[0088] Polymorphisms in DNA sequences can be detected or typed by a variety of effective methods well known in the art including, but not limited to, those disclosed in U.S. Pat. Nos. 5,468,613, 5,217,863; 5,210,015; 5,876,930; 6,030,787; 6,004,744; 6,013,431; 5,595,890; 5,762,876; 5,945,283; 5,468,613; 6,090,558; 5,800,944; 5,616,464; 7,312,039; 7,238,476; 7,297,485; 7,282,355; 7,270,981 and 7,250,252 all of which are incorporated herein by reference in their entireties. However, the compositions and methods of the present invention can be used in conjunction with any polymorphism typing method to type polymorphisms in genomic DNA samples. These genomic DNA samples used include but are not limited to genomic DNA isolated directly from a plant, cloned genomic DNA, or amplified genomic DNA.

[0089] For instance, polymorphisms in DNA sequences can be detected by hybridization to allele-specific oligonucleotide (ASO) probes as disclosed in U.S. Pat. Nos. 5,468,613 and 5,217,863. U.S. Pat. No. 5,468,613 discloses allele specific oligonucleotide hybridizations where single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process in which the sequence containing the nucleotide variation is amplified, spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe.

[0090] Target nucleic acid sequence can also be detected by probe ligation methods as disclosed in U.S. Pat. No. 5,800,944 where sequence of interest is amplified and hybridized to probes followed by ligation to detect a labeled part of the probe.

[0091] Microarrays can also be used for polymorphism detection, wherein oligonucleotide probe sets are assembled in an overlapping fashion to represent a single sequence such that a difference in the target sequence at one point would result in partial probe hybridization (Borevitz et al., Genome Res. 13:513-523 (2003); Cui et al., Bioinformatics 21:3852-3858 (2005)). On any one microarray, it is expected there will be a plurality of target sequences, which may represent genes and/or noncoding regions wherein each target sequence is represented by a series of overlapping oligonucleotides, rather than by a single probe. This platform provides for high throughput screening a plurality of polymorphisms. A single-feature polymorphism (SFP) is a polymorphism detected by a single probe in an oligonucleotide array, wherein a feature is a probe in the array. Typing of target sequences by microarray-based methods is disclosed in U.S. Pat. Nos. 6,799,122; 6,913,879; and 6,996,476.

[0092] Target nucleic acid sequence can also be detected by probe linking methods as disclosed in U.S. Pat. No. 5,616,464, employing at least one pair of probes having sequences homologous to adjacent portions of the target nucleic acid sequence and having side chains which non-covalently bind to form a stem upon base pairing of the probes to the target nucleic acid sequence. At least one of the side chains has a photoactivatable group which can form a covalent cross-link with the other side chain member of the stem.

[0093] Other methods for detecting SNPs and Indels include single base extension (SBE) methods. Examples of SBE methods include, but are not limited, to those disclosed in U.S. Pat. Nos. 6,004,744; 6,013,431; 5,595,890; 5,762,876; and 5,945,283. SBE methods are based on extension of a nucleotide primer that is adjacent to a polymorphism to incorporate a detectable nucleotide residue upon extension of the primer. In certain embodiments, the SBE method uses three synthetic oligonucleotides. Two of the oligonucleotides serve as PCR primers and are complementary to sequence of the locus of genomic DNA which flanks a region containing the polymorphism to be assayed. Following amplification of the region of the genome containing the polymorphism, the PCR product is mixed with the third oligonucleotide (called an extension primer) which is designed to hybridize to the amplified DNA adjacent to the polymorphism in the presence of DNA polymerase and two differentially labeled dideoxynucleosidetriphosphates. If the polymorphism is present on the template, one of the labeled dideoxynucleosidetriphosphates can be added to the primer in a single base chain extension. The allele present is then inferred by determining which of the two differential labels was added to the extension primer. Homozygous samples will result in only one of the two labeled bases being incorporated and thus only one of the two labels will be detected. Heterozygous samples have both alleles present, and will thus direct incorporation of both labels (into different molecules of the extension primer) and thus both labels will be detected.

[0094] In another method for detecting polymorphisms, SNPs and Indels can be detected by methods disclosed in U.S. Pat. Nos. 5,210,015; 5,876,930; and 6,030,787 in which an oligonucleotide probe having a 5' fluorescent reporter dye and a 3' quencher dye covalently linked to the 5' and 3' ends of the probe. When the probe is intact, the proximity of the reporter dye to the quencher dye results in the suppression of the reporter dye fluorescence, e.g. by Forster-type energy transfer. During PCR forward and reverse primers hybridize to a specific sequence of the target DNA flanking a polymorphism while the hybridization probe hybridizes to polymorphism-containing sequence within the amplified PCR product. In the subsequent PCR cycle DNA polymerase with 5'→3' exonuclease activity cleaves the probe and separates the reporter dye from the quencher dye resulting in increased fluorescence of the reporter.

[0095] In another embodiment, the locus or loci of interest can be directly sequenced using nucleic acid sequencing technologies. Methods for nucleic acid sequencing are known in the art and include technologies provided by 454 Life Sciences (Branford, Conn.), Agencourt Bioscience (Beverly, Mass.), Applied Biosystems (Foster City, Calif.), LI-COR Biosciences (Lincoln, Nebr.), NimbleGen Systems (Madison, Wis.), Illumina (San Diego, Calif.), and VisiGen Biotechnologies (Houston, Tex.). Such nucleic acid sequencing technologies comprise formats such as parallel bead arrays, sequencing by ligation, capillary electrophoresis, electronic microchips, "biochips," microarrays, parallel microchips, and single-molecule arrays, as reviewed by R.F. Service Science (2006) 311:1544-1546.

[0096] The markers to be used in the methods of the present invention should preferably be diagnostic of origin in order for inferences to be made about subsequent populations. Experience to date suggests that SNP markers may be ideal for mapping because the likelihood that a particular SNP allele is derived from independent origins in the extant populations of a particular species is very low. As such, SNP markers appear to be useful for tracking and assisting introgression of QTLs, particularly in the case of genotypes.

Sequence CWU 1

1

1471301DNAGlycine maxunsure(1)..(301)unsure at all n locations 1ttgctttcat ttgttacaaa gcaacaattc tctctttagt acatgttgag caaagtgaat 60caggcaactt atcaaagtag tcccatgttg catgataaag aagaggaaaa tagaaagaat 120tttcagttaa ccgaaaaagt acaatgtncc ntttagataa attgaaaatg aaacaatatg 180ttatcatgnt tctatcctcc aattcataca tgaccttttt gctttacgtg aaggaggacc 240tcatccaatt gttggataat caaaatgaga aaagtcctca tctnaatgat tatttctctc 300g 3012301DNAGlycine maxunsure(1)..(301)unsure at all n locations 2ttaacctnct atatgtatct cagtctcttc cttttctttc atttgctcat aattagaact 60ataaaatagg cttaacccga caaactgacc cattagcctg ttagggcggg tcaagctggg 120cccaagaaaa ttcggcctga atagaacttg gtcaatctgg gtactctgat aggcccagac 180cgagctcaag aattacaaat ccaagttcaa tcaggnctat tatagtccaa cattattttt 240attttttatc ctttaaattt atataaatcc agacaaaatt ttaaaacggg ccattgggct 300a 3013301DNAGlycine maxunsure(1)..(301)unsure at all n locations 3gtatacagaa acagacaata atctcttact taattttcca ctagtcattg tttaacccaa 60tgctggaaag tgatctttct aaagactcat tttatcatat tgaaaagtag ctttgaccga 120ataaagcatt gtacattctt ttttgtgctt tgcggtaggt tccattggaa tgcacttcga 180tatgtttaca tctaaatcca attttgggtc atacattcat tattatgttt ctaagccata 240aatataagat aagtgttgca tatatttaag tttattggat tgcacttcgt attatntata 300t 3014378DNAGlycine max 4agatctaaca aggtaatttt tcccttaact atttgaaact cattatcaca acttggtaaa 60agttaccttt caattgtcaa atttaaaacc catcacataa aatgccacaa atacatgcca 120caccaaaatt tgcaagagaa ttcaaccaca ctgcagatct gcttcaggta gcagttcatc 180gtgatacttt caccatactc aagcagaaag gtatatacaa agtactcatt tttttttgtt 240aagaatgtga gggtaaagac caatttctga cccaatctgt aagagttgcc acatttctac 300taacgtcttc tatattatca ctcttcattg caatcacttt ctcctccgag taactttctt 360tagcctcctc aagcaaaa 3785760DNAGlycine max 5gccttgggtt cttgatgttg ccaagaaatt tgggctactt ggtgctactt tcttcactca 60gacatgcaca acaaacaaca tatacttcca tgtttataag aagttgatag agttgcctct 120tacacaggca gaatatttgt tgccagggtt gccaaaactt gcagctgggg acttgccatc 180tttcttgaac aaatatgggt cctatccagg ctactttgat gtagttgtga atcaatttgt 240caacattgat aaagcagatt gggttcttgc aaacagtttt tatgaactgg agcaaggggt 300aagtgacttg actagctgaa aattctcttc attaatttga tttgtactta tttgttggaa 360tgaattagac agcagtagtt atctgggtaa aaagtttttc cttcaaattt tggtcaaaat 420ttgtcctagt tcctattact tcaggatgat tctggtcttc ttttttattt ataattgatg 480gattttgtct ctcatcctag tatcctaagt tccataaaaa gagaaaaaat tcatcaatta 540taaaaagaat tcaaagacca aaaatatcat ttttaaagta taacgactaa gaacaatttt 600gattgaaatt agagagatct agaatacatt tttatcctag ttattttgtt ggataatatt 660gatgattgaa atttgaaatg aaatttattg taggttgtgg attggctggt gaagatttgg 720ccattaaagc caataggacc atgcctgcca tctatctact 7606301DNAGlycine max 6taggacccat atttgttcaa gaaagatggc aagtccccag ctgcaagttt tggcaaccct 60ggcaacaaat attctgcctg tgtaagaggc aactctatca acttcttata aacatggaag 120tatatgttgt ttgttgtgca tgtctgagtg aagaaagtag caccaagtag cccaaatttc 180ttggcaacat caagaaccca aggcatgaaa gcatcataga taacacaatc tggagggtgg 240cttgaccctg caagcttctg aacaagctca gcaaaagttt gtgatccaac cctccaaaag 300g 3017301DNAGlycine max 7agaacagaac aattcattta tgcggttatt gtttggtttt gcaggttaga gctgcagagc 60tgaggaagat attggtggag cttgggccgg tatgtaatgc tggattgaat gcataataat 120tcagttttag tagtggtttt tcttaattgc tattttcttt taattggttg atttggttca 180ttgctgtttt atactacttt tttgcaggca tatatcaaaa ttgcccaggc tatatcatcc 240cgtgctgtaa gttttggttt atctgtcaca ttattaaaac tgatctccat gttctttggt 300t 3018301DNAGlycine maxunsure(1)..(301)unsure at all n locations 8tatcctttgc gatggtttgg gcatgaatac ttgatactta cctagagaaa aggttaaatt 60taatatcatc atgatcttct tcctaaatga tgttgtgtgc cttttgagtt tcgagattta 120gttcttggat atatgcctga agtgtttctc ccttgaaaac aagttaaggc atatagggaa 180aatttgtctt tacacattct tgtttgttgc ttgctgaaaa aagttgagaa ttctgctgat 240gcactatggt atggctattg tagggggnaa agttgtctga agtgaaggat ctctacctga 300t 3019301DNAGlycine maxunsure(1)..(301)unsure at all n locations 9ttttcttgtt ggntcgtaaa ttgcaatatg gggatctttt ttggatttta cttgttttat 60tatacctgac ttttagtcaa tcgttcagca ataggtatat taaacttgga acgctanatg 120gacaatcatt ttactgtttt gtttatccta ataaaaaaaa ttgtactttt catcaatgac 180atgtaggaaa gcanacaggc cagtcctgac aagaatcaaa tactgtacat ggaggagttg 240tcgttagttt taaatcaagt tgaatccatt caagacattc ttcctataat ttctgtcatt 300c 30110301DNAGlycine maxunsure(1)..(301)unsure at all n locations 10aatgaaccaa tttgaaggaa acaaacaaat taaagctgta aaaatnaana atatccaatt 60attaccccgt gttcttttag aaattgaatg gaatcatgtg tactgcacta taaatgacca 120ccaaataaat ggtttattta acaaagtgga atccatgtct tcaaacaagt agcacgaagg 180acatcaaaac catagttcac tgaaggacgc tagtttaaat ttcatcnttt tagtgaactt 240caatgtaatt aaataaatca aaggatggga tatacatgtt gtttgaagac tcattggacg 300g 30111301DNAGlycine maxunsure(1)..(301)unsure at all n locations 11tttcttggtt gaaatttccg caacctcctc ctttgtaatt tccttgacct ttacctctat 60aatttcctct tcctccattg ttgagattat nacaaacttt gctttgtgca ctttgacttg 120attcaacaac acttttgaga ttcacctgac tctttaaggc ttcttcagtt actttttcaa 180tcttcttcaa gattctactc atgtgacttt ttgatgcttc cctgcaaata tgctattgtc 240gtggtgtaga atgggatggt gttaggattt tgtccactac cttgttgtct aggatgtctt 300c 30112301DNAGlycine max 12gtttactaac tactatatgt tttgttgtgt tttgtataac ttcattcaat tgtacaggaa 60tgaaaatgaa tacgatttta ttgggaagat tgttaaagag gtctcccaaa aaattaatcg 120cactctttta catgttgtgg attaccctgt tggattggag tctcgagtgc tacaggtaat 180ctcactttta gatgatggtt cagacgaagt ccacatggta ggaatccatg gaattggtgg 240ggtaggaaaa acaacaattg ctcgagcagt ttataatttg attgctgatc aatttgaagg 300g 30113301DNAGlycine maxunsure(1)..(301)unsure at all n locations 13ataaaatatt attttatttt attaaaaaga aaagaaaaaa ataattttca ataacaacaa 60cttaattatt tttatattct taatttactt gtgaatgaag cttctgatga tgcaacatat 120gtgatgagta aagaaaagag gatttcaana actcgttgat gtggaatacc gagagtatct 180cattgattcg ttagaatacc aagaggttga tataagggtt tgactgctcg ttgattcaag 240agaaagccga gggttgagaa ttgagaaaaa cttnaaattt ttgttcaata ataatctttt 300c 30114301DNAGlycine max 14tgacattact aatgtgcaca tttttgtggt taaattgtgc tttggacctg gaagggaact 60gataaaatag aattcataaa gcttgaaggg tacaacaaca tacaagtgca atggaatgga 120aaagccttcc agaagatgaa gaacctgaga attttgataa ttgaaaatac aaccttttct 180acaggccctg agcatctacc aaatagtttg agatttctag actggagctg ctatccttca 240ccatctttac catctgattt caatccaaaa cgagttgaga tactcaaaat gcctgaaagt 300t 30115409DNAGlycine max 15ccaagatggg aaagagttga gtttagaagt gtttccaaag gccattcttg tttgccatct 60tgatcccagg cattatcatc atcttgacct gtattatctg tacatgagtc ctaataatgt 120catccaagta tgcagtccta atctgtatat gctctgtgat ttcgatttgt tgttccaaaa 180gcttgcattg ggtgtcgaga aggactggtt tcgcagatgc aggaaatggt caatgaattt 240ctcgtttcga aaaaagtttc ctaagattgc ggtatgttgt tctataatat ctagattgaa 300aagtgtcatg gaaatggtgt tgattttgaa gttcagcgta ctcatcaatg gcactatgca 360atttagttct tcatgtaatt acatatttag aacatgggac ccgataatc 40916301DNAGlycine maxunsure(1)..(301)unsure at all n locations 16agacaattaa taagcatggc ctcntccaac tccaagaaat gctaccctct gaaatactca 60aggagaaata tatcaaagtg ggagatgttt actgagtaat tccaataata aaaaggaggc 120ttgaacaaaa gaaggttctt ctggttctag atgatgttga caaattagag cagttaaaag 180tacttgcggg acaatatgat tggttaggct ctggaaggac aatcattgtc accacaagag 240agacaaacac ttgctagcta ctcatggagt gataaactta tacgaggtta aaccattaaa 300t 30117301DNAGlycine maxunsure(1)..(301)unsure at all n locations 17attatatcta taataattta tgattatacg taagagattt tgttacatta ttcgtacata 60attattaata aaatgattta nttanatcga tagaaaagtt agaaatccac caaaaatcat 120tttaacattt ttttcaaatc tattgattat ttttaacaac atattagnaa ttcgtttaat 180ttaacaatat attagtaatt tgtctaatta gggcataact ggttggattt ttttatttaa 240aatccaagca tatactcatt ttggcccatt ggttacgcaa aaactctaca tatgccgtaa 300c 30118301DNAGlycine maxunsure(1)..(301)unsure at all n locations 18tattgttagc acattgccct ctatcagttc atncctcaaa tctgagcaaa ctaagctttg 60atgtctctca cataaaccga ttcaacacaa caatgtcatt ttcattgtca agaaggttca 120gctcttttct ttcaaacctc acttcccctc cttgctttca cttagcctcc ccattgacaa 180tgacaatgtt gttgatgatg tgttccccaa ttcaatgaca tgctccttgt gcgtcatacc 240ccacccacca tctaatttaa caagatgtta ggatcattnt aaagatgaag cattatccca 300c 30119301DNAGlycine max 19gcagtcagat ccagcatagc atgaattgca ttcacaacga gacttattca tatctttaag 60aataagacca tctaagtagg ctcttccatg tcctgaacat ggtattgctg ccacagcctc 120agcttcttct gcagctcttg tgctccaagt tggctcccat tctcccatac atacaaacgt 180gctaattgag gcacacaata taataataaa ttttaatttc attggacttt ggacttgttc 240ttttggcgat tctagtcttc gtttctttgt gaaatcttca agcaatttct tggttgcact 300t 301201154DNAGlycine maxunsure(1)..(1154)unsure at all n locations 20tgattcgcca gcttgctgcc tgcgggcaca tttctccata tcagaccaca cctctctttt 60ctgtaagcct cttattggtt ccctaacgaa tgttgttaat tacattgttg ttctgactat 120ttattcagct tcggttgtag ccacggattc atgacagaga ttatcaactc atgcttagtt 180cgaagactat tttaactgtt ttagttttga gttcagaata aagcacgcag cagaggtatc 240tatctcctca gcttaaagct tttcaattca tcactttgaa tattattttt gtttttttag 300tgagctataa cacaatcaga cttgaaaaat agtatcttat agaaaagtca attacaaatt 360ttcttgtgaa atataaccac taaatcacat gaaaaaaata tgnaataatc gagaattaaa 420aagttgagtt ttaggattta aggttttatc tccttttaga aataccagag ccatacaatt 480ggtgtgcagt cttctccaat cttctatctt tattacattt ttattttgtt tttctaattc 540tttttttaac tatttaatta gttagnttta ttttgttttt cattttttta attagttaca 600tgacatatca caaataactt taaaattgct gactgatatg atcgaccaca tgatcacatt 660taactgcaca agcaaattga gttaggaaaa naattataat ctgatatttc taaaatttta 720gaaattaaat gcaaagaata aaattcaaag gactacatat aaatttggtg tattttcaga 780ataaaaaaac atatttaatc cctctatata ataatgaggt aggaacgggg aagaattttc 840aaatattaat gtgaatacaa ttttctccta tagttcatta tatttcgacc gtcttaattt 900gtccttactc ttgaaaactt attaagctga aatttttacc tatatctaaa tgcatggaca 960acatggctac ttcacttcag aaatctcgaa gtgcaaccaa gaaattgctt gaagatttca 1020caaagaaacg aagactagaa tcgccaaaag aacaagtcca aagtccaatg aaattaaaat 1080ttattattgt attgtgtgcc tcaattagca cgtttgtatg tatgggagaa tgggagccaa 1140cttggagcac aaga 115421301DNAGlycine maxunsure(1)..(301)unsure at all n locations 21cctttaaaac gttacaccat atccattggt ggaagaggag ggatggaaaa gnaagggtcg 60ttgcttaagg acttnttctt aggttaatga cattaatatg tgaaaattta aaatacattc 120tttttaattg acgttacata acatgattgg aggggcatga aaagggacag acttttagga 180ggaatgtgat ttcaatccgt ttgaaataat aagaaatata ttagaacggg ttgaatatta 240gataatttat tataaatatt tattttaata aaataagtat ttttttacta aattattttt 300a 30122910DNAGlycine max 22actaaaacta cctcaactgt tatagatgtg gtcatttcac ttaaatactg ctttagcaaa 60tgtccatagc aattgttcag tgtcaatgct gctactgcac cagctattgt gttccatttc 120ttcaaaattg gactataatt ttgtctctct ttcaatgcca aatattcagt ttcctgagct 180aattgaagca taacttcacc tatttctttc tttgtttcag actcagcaga tttggcattt 240gctgcttcca tcatctacag tcattaggca acaaagacat tatgatattt tctttttact 300tttaaattaa aaagcagaaa atactataag tttgacacca ttagggatta aaaagcagaa 360aatactacaa gcagaaaact ctacaagtta tatacatgct tctaacgtat caaccttttg 420gattgtttct taacaaatac ctaccttttc aaaagcattt ttcaaggaag aacagatata 480gtcatcaatc cggccctcag aggaattcgc tctagttttt tctccttttt cttgcctttc 540ttcagaattt gtaacatctc ccaaaatctt ggatgctaat aacaccacag gggagaaggt 600tttcaatttg tccaatatca ccctccctcg aaatacctct gggagttaaa gaaacctttt 660atctgccccc ttcttggcgg gaactgagga cacatttcaa tatcttcaaa ataaccagga 720ctccccttct ctttcttaaa ctcatttgcg ccctccattt ataatgtttg gaaggcaaaa 780aagggaggtt taatttccca tttctcattt gtggaacatc ccctgaaaac acttgtgtgg 840gctttttcca cggggccttt ttttaaaaga atgtcctttt taaaaaagta tcactctcaa 900acatgcggga 91023301DNAGlycine maxunsure(1)..(301)unsure at all n locations 23tcccttagct gaaanaaaag gtcaatgtca gtatagggac atggaagtct ttcattgant 60attactaatg gagttatggt gtttattttt gtatgtccaa tttccatagg gcatagtgcc 120actcaattct ntaattgata tagatttctt ctgagttctc tttgtaaatt tccagccact 180tgtatgtgca gtggtctttc atttttttgg tttgctataa tcaataggat taatacnaaa 240gtgctgtttg gttagtggtt ttcaaattta cagttcagta taaagttatc caggtaatac 300a 30124301DNAGlycine max 24attggtcaaa ataaaacgtc cacgtgcact tgaatgatga gtttgaaccc ttattttgtg 60tgtgtgaatg atgatgagta tgaagctatt atgtaaaaga tgatgtgaac aaagattcca 120ataatttaaa aacaggcaag tggcatatat tattagactg gagatcactc aagtcaaatt 180taatgttatt agttattact agctagaggg atagttacgg actttgcatg gtgagggaac 240tcaatttaaa tgtaacaaga atttttattt tttttaagta acaagaaaaa attgaataaa 300a 30125864DNAGlycine maxunsure(1)..(864)unsure at all n locations 25ttgttttctt ctctgtattt acaatagggg aaagatgcat gaagcaatac gtcttgtgtt 60gtcttagtga gatgctttgt ctcatggaca aaggagttat aaagctttat aatctgacag 120ttttttcttt tttcttaccg aactgtctgt ctgctcaaga tggtgcatgc ttagttttgg 180tttatggaat tcaggaagat aaggaagtat cttgaatttg agcttttaat caatttctat 240atgcntattg tgatggtagt tttcatttaa aacaaaaatt gtgtttgtca gttggggtta 300caatcactgg atcgcatcat ttttatgttg gatggcattc gtgtcaatat attttatctg 360gttctgtgct aagctaccat ggaggagttt atgtcttgtt ttggtgaaat ttcttctctt 420tgattaaaag tgaagttaaa ctggaaaaaa atgagattta attaatgaac gagtgacaaa 480tggtatgggt ttggtttttg gtttgtttat attggtttaa catgggatgt tgcgatgata 540tagaggggtc catgctatgt tctgtatgat accaataagc ttgtggtaga gtaaactcca 600aaaggatgaa gaaaaaagtt agcataaggc atagagtgca ttattatgtt aaagaatgag 660aaattgcaat ggcttagagt gcattattat taactatatg ctggaatata tatgataagg 720ctccattggt ttatgagaaa ctacgggtgg agattttccc ccctattaaa tagtaacaaa 780tttggaagat taaatacaca aatgttcact tctcttgtgt atgttggtct ctcagtgctt 840gcatatggta gtaatctgca ttat 864261068DNAGlycine maxunsure(1)..(1068)unsure at all n locations 26cccaggcatt aaccagggag atggcacaaa gttgaacaat cacattaaaa ttattatttt 60aaccagctcc accaatgaac catactatca aggctctatc agtttgcttc caaagtcggg 120cttccttaca caaacagaca agacaatgct tacataagac agacaaaact gctcatttac 180actgtttgga agggatgaat atgggaggga agaaacacag ataacaatgt catttccttt 240gtttggttgg agaggaagtg gaaagaaaat gagaagaaaa gttgacttct agagataaaa 300attcaaactt ttttgtactt tctctccaat ttcaatttca aacttttctc tcctccctca 360ctttctttcc tctcgaccaa acatagggct aaagttgcac aattcaaaaa attacactct 420cccaaagcaa ctttgacaaa ctcaaccaat agctttgacc taactaagag acacnagctt 480gttcacacat ctctccccca ctagaaaaga caaaactcta cagaaaaagc caaaaaccag 540ctgcagctta atttcattta gcattgtgct tttggatcca ctataggcta ctcggatcca 600atggttgttt caatggaatt gtgacttccc tctcttcttt gtaataaata aatctttttt 660tgtcatcaaa tatatgtaaa acttatcctt gttaaatata accacaactt cctaataata 720tactaccaat tttttttaat caataacaag aaaaaaatgt ctaaccatta gtcatttgct 780atgcaagtga aaacatcctt tggaacccat gttatccgaa gtgacacgat gatttgagtc 840tcatatgtga gaatcaatat ttaacattta attttagtag ataaataaac cccaaaagat 900aggaggtaga aaccatgctc agatgtgtat aaagaaggtt gtaccttttg ggactttttc 960tttttccctt catcagcttc ttcactctct tcactttcaa catatgatac cttcctgact 1020gtcctactag aagtgcgaat ctcactgttg cgcgaagaaa aacctgtg 106827301DNAGlycine maxunsure(1)..(301)unsure at all n locations 27tgttaaaagt ttaaacgtcg tataatctga aatggataaa gtgttttttt tnnaaaaaaa 60tatttaaata ttaaaaattt ctctagagaa acaatcttat attataattg ttatcaaatg 120gttctataga ttatatatat aagcaaaatc tttatgttag tgattctaag gactttaaaa 180cttcaataat cctcggaagg ttataatatt ttaattctng aattacattt tgaatcaaat 240agttcaacag ttttgtttct taacttaaaa tcaaattgat gtttcacacc aatgagtctt 300g 30128301DNAGlycine maxunsure(1)..(301)unsure at all n locations 28ctttgtcacc atgttngttt cacagtcacc tacngttatt tattacacaa attcttnacc 60atgcattacc tttttaagtt naggcaaata tttttatatt tttntttacc aaatcaaact 120ttatgcaaat taggaataaa aataagttag attactcaac gggaggcttg gccaatatga 180cttaacctac atcaggccta tgggtcaaat tggacttttt cngaaaataa attattccaa 240gactttcatt aaaaaanaaa aaacaaaaaa cacatatatt tcaaacttaa aaagaaaaaa 300g 30129301DNAGlycine max 29aatttaggaa aaagacacgt atattctcta gttttttatt ttattttgaa gactttcata 60attctttttg ttttagatta ggatgattaa tcaaagtaaa caccgcccct agctttcttt 120ttaacttgca ttacggataa cttaaccccc ttgctttgca cccaattaaa aagcaataca 180ttcacgtgga gatacgagag tgactacata tgatttgtgg gagagaaaaa ggaataagaa 240ggaaaaagta aatgaatgac atggataagt acatatatgg gtcctcactc atattagtca 300c 30130301DNAGlycine maxunsure(1)..(301)unsure at all n locations 30ggaaccaaaa ctaccagatg taacaactcg tgttgaatca tcgatggctc

ccatgtacct 60cagcatacca tattcaagtt ctgcaaatcc ctgcacttca atcctcttag ttgaaggaac 120attcaagttc aaagtctgtt aatagattaa aaattcactc atctgctaaa ttaactattt 180tgnacattac tattggtata aaatgtcaac ctaacacatg agtcacctac taactagtga 240ggtctcggga gggtagatat actcagctcc accctcataa gctgagaggc tgtctctagc 300a 30131301DNAGlycine maxunsure(1)..(301)unsure at all n locations 31aacttgagtg tntntttttt tcctttcaaa tattcttccg ttctagtccc tgacctaagt 60ctattttctt gctgtaaagg ttttttcggt cgattggagt ccagatggag agaaggtagc 120ctctggtggt aaggataaag tgttgaagtt gtggatgggc taggctaatt tttggatgan 180tattgggaat ccaacgaagt acaatctcaa tggagttttg cggatgcatg gatttcatgg 240aaatcaatgg ttggtattat gtggatgcaa ggtctttaaa ttatatagac agcatagaag 300a 30132301DNAGlycine maxunsure(1)..(301)unsure at all n locations 32caaaatgaaa tattttattt tactctnttt cctttttaat tacactcttt cagtaaaaan 60aaaanatgcc atgttttaaa ccttgtatcc ttattttaat ttttatttat aaaatacncc 120agatataaaa caggacgtac attctgtcat nttttaacac cttcgggcat atatgagaag 180aggattctgt gttttgactt acacaagtac aacaaaggcc atgttgttca gtgacagtac 240tgccttattc agttatttac cttttcaaaa taaaaaagtt atttacttgg agagtttcca 300a 30133301DNAGlycine maxunsure(1)..(301)unsure at all n locations 33gccgggttaa gattatcctt atatccaggc ctttttattt gctnatgaat aaaacactaa 60tcgttacaat aattgaggtg gacaatatga gatgaatgaa ttttcaatta cttcattcaa 120cctaagaaat antgatgctt cttttcntga gtctcctcat gttctttctt ctcatgatgc 180tcaggatctt tatgggcctt ctgcctctca tgctgtaggc atagatgatt aaacaaaatt 240tgccatgtta tttaatattt gttactttaa aaaatnataa caattcacca aacttaaatg 300a 30134301DNAGlycine maxunsure(1)..(301)unsure at all n locations 34tataaacatt tagatttttt atataagaaa ttattctcaa gaatccactc tgtcatgtca 60gcttgtttca atgcaaaaaa aaatattaag gaacggtttc ttaatttcca ttaagcactt 120ggaaagaaac ctgcattgga gntggagatg ctgtaaggag tcactcttag gtaaagcttt 180gtgtgaacaa tttgtgcacc anaaactatt tcccttctat atatatttca gaaaccaaaa 240aacaatttgt gcaccacaaa aattaaacta ataaaaaaaa ttataaaggt ggtggaggng 300t 30135301DNAGlycine maxunsure(1)..(301)unsure at all n locations 35gcatggttct cgacgtttac tgattttgtg ggttgtcacc ttgtggagga cccaccttga 60ctgtgcttgt acgaggcttc tttctatttt gagcaangct aattaaccac acattattaa 120aaaaaataca aaattttnat ggtttttctt atttatttta ataaatttta atcaattata 180atagaaatat tttagaaaga atgagatcac tgggaaaaac tttaacgcat gaaagcatac 240aacttcatgc gatatagctt attcgtggac caatattggg ttaggatcct ttcttcattt 300t 301361185DNAGlycine maxunsure(1)..(1185)unsure at all n locations 36gcatcttgag caccttgggg nattggtgct gcggctgccg gtgcttatgc cttggnnnnt 60nattatatat cttccttcaa taatatattt tgttcacgat cgtttattta atttgaaata 120gatttatata ttacttatgt gagatgattc acaccccctt tttatatatt ttagctttaa 180aatgttacct tcaccagaat aaataaaaga agtgcaaact ctttggtaat cgagggaaat 240atatatacct cccccacata tacatcatca cttagacttg gacgtatcta aatcggttaa 300ttttaatatg tttatatgta tgcgtgtgca ttaataattt tcatattttt ttttgtaagc 360attttaaagc cttacatatt gaaaaaattg tcattaattt gtgttttgga catgaattaa 420tcctatcatc ttgaatcatg tccacaaata atttcaattt gacattttct ttttaaggcg 480gccaacatat atacatactt gatctttgta cttttggatt gtgatgcttt aataattgtg 540gataatagat ataaaaatat attatagcta tatagtatta tttctctcta cccactgtgt 600gtaactatac tgtctataca tctcatgtgg tttgtttttt cttaaatgaa aattgttggg 660gtcatgggtg tatagagtat agtactttta tgacgccatc agaagagaaa caataaaagt 720tcataaaaaa ttaggtgtag aaaaagatgg aacttaagaa agaaaaaaga gagagagaaa 780gtgattaagt gatgtaatat ataatgagaa atgaagaaaa agataggaag acaaataaag 840taaaaaaaag aaagaaagaa agaaagatat ataacaaaaa attgaaatgt atattctaat 900atgtattgaa aacaaaattg atcccttttt gctgcaatgg ttaattttat gacagcatga 960gaagcatgag gccaagaaag acccagagca tgctcacagg cacaaggtag aagaggagat 1020tgcggcagca gctactgttg gtgctggtgg ttttgtcttg catgaacacc atgagaaaaa 1080ggaagttaag aaagaagatg aggaagctca tggaaagaag caccaccatc tgtttggctg 1140aacatgataa atattcatat ataattaata ttcaagctcc aaacg 118537301DNAGlycine maxunsure(1)..(301)unsure at all n locations 37ctaatggtag aggaatccac gcattctagg aaattaagct ccctaccttg tgccatatcc 60tcatactgca cctccacatt caacattaaa tcaacattta gccnatccgc aagagtaaac 120tttgctctat tcatcattgc ntcaacttgt tccaattttg cattccacac catttcattc 180attttcttcc aaatgttgca tatactcatg gccacaagat ctatcacggt acataactac 240tggagaggat aggcacatcg cattgcacaa cgcagagggg ttcttgttaa ctcaacattt 300g 30138301DNAGlycine maxunsure(1)..(301)unsure at all n locations 38ctcctctcct cattagttgt agccctctaa gctctaaaaa ctctctcctc tcatgttcca 60caacttgatg aaggaagagt tcaagacttc naagttagtg atgatacttc acaacaatat 120tattcacagt tcaatatgtg atatcgtaaa cttgtttcga cctttagcaa catcctacct 180taggtcaatg ttggtcgacg atgaaacctc actactacaa aatcattttt ttacgacgca 240nattntaaga ctgttattaa taaccatctt agaatgtgtc acaatgacat ttttgtaatt 300a 30139301DNAGlycine maxunsure(1)..(301)unsure at all n locations 39taactaactc tcaacaactt aactgtcatt ctttctcttc ttattcttcc tactctacat 60tagatgattt ctcttgccta accgtttttt ctttactatt ttctttcctt ccatccaaac 120atacaattaa tgtgcatttc acttngcttt gaacaacact taaccgttta tgctagctcg 180aagttggatt gaggtatctt ttacgttgaa cggggactga gctttaaatc caatctagaa 240caaataatat tttgtatgta cgtattatta tatgaaaaaa aaggttttta aaagtagaat 300t 30140301DNAGlycine maxunsure(1)..(301)unsure at all n locations 40cattcacaac aaaagccaaa acccttttag aggctcaccg gcgaagaagc tcaacaactt 60ctctgatcaa aatcttcaca aaatctgaca cccttagaag caaagattga aactttntct 120caaaatcaag ctgagaaccc tgaaacaaag acaaacaact aaaaagaaca catcctcagt 180caccaaggag tgaagaagtg tcgtaagaaa acaagggaaa aagagaagaa aaagagcgaa 240atcactaaag acaaggatta gtttgtgatg ngaactagtt actatgtaac naggctatat 300a 30141301DNAGlycine maxunsure(1)..(301)unsure at all n locations 41aaaagacaaa gaaagaaatn atgttaagca tncaaaactt gatgtctaag tttatgttta 60tgcttttgnc aatgttgaag tgaagctacn tgtaaggatg ttcacgagta tgagccactc 120naattgaccc aaacacgttg gatgtttaat gtgttttagt tgagcccaaa tcaatccatt 180caaactactt aacttttggg ttgtgtcaca agattttaat tcttgcactc actgacccga 240cccatgaaca tctttttaat attaaattat tatatatata tatatatttc ataatatata 300t 30142679DNAGlycine maxunsure(1)..(679)unsure at all n locations 42ataatttgat catcatattc ctaaactttt ntgcttaagc agagacaagc tgctcaatgt 60gtgggtcgtg taatccgttc aaaggctgat tatggaatga tgatttttgc agacaaaagg 120ttagtatcct tgagtccttt tgcttccatg aacatgttga acatttggga aaatgtgagg 180gttgcttata gtattctacg ctgacgttaa tttgatgaag ncaacttgct agtttctagt 240tttgattcaa aaagcacaat gcactcttct ctttgtaact ttatggaatt tgtcctgaaa 300tggattggaa gttattggtt gtctctaata atgaatgaca aaccaaaaga aacttgacaa 360tcttcagaat ccagtttgtc aagcaaagaa agaaatatgt ttgttcaggt ccatgacatg 420tttagtttca aaaaccaatg taactaacag atataannag caaaaattgg taaatgtctc 480tgtaaatatc aataattaca gtcacaaatt ctatacattn tataggaaat anccaattta 540aacttatgaa agtctgcatg aataaatggc ctattttacc tatgatgatt gctccattta 600tggttttggc ttctactttt ctctatgaca gtgttctgac agtaaatagt aatacattgt 660tggttataac ttctgcact 67943744DNAGlycine max 43ctaagtacga aaatcgttat ccgccgcaac agccactaag atgttctttg agtcttggca 60cgccacgtgt cagaactgcc aacagatagc cccatctctt tctccttctc cctaaacctc 120gaactcagca cccccatcca ctggtccctc cccactccat catttattat actttcttct 180tcttctttat tattgttgat taatataaca tacacccaca tatttcatat gggtacttgt 240taatttgggt gtggattgtt agtttgttac ttgttttgtt ccgttcaggt gattgtttga 300ttgagccttg aagaaatgga ccacagcgct gatgcacatc gcacggactt gatgaccata 360acgcggttcg tgctgaacga gcaatccaag caccccgagt cacgcggcga tttcaccatc 420ttgctcagtc acattgttct cggttgcaag ttcgtttgtt ccgctgtcag caaggtaagc 480tatccctact ttgtgtgttt tttatcgaca aatattaatt gttagtatta ttaatccttt 540ttcttttctt ctcttttcgt cattagacta atcttatatc tcgttatcat gtatttattt 600cactctattc aaatatatta ttctggtctt aaatataaga agaagaaaaa aaaatgattc 660atgctgacta agaatgaaat attgattaga gttatgtgtt gtgacctgtg caggctggtc 720ttgctaaact tattggactc gctg 74444301DNAGlycine maxunsure(1)..(301)unsure at all n locations 44accaagacgg tcatagacaa cgaccntcat tgtgttcatt caatttattt acaaaattgt 60cattangtgg cattctagga cggttcttta tgatggtctt aaaacctctc acgtaaataa 120taattataat nncattaatn acaanaatgt cactatgtta ttttctaaga aggcggctct 180acaaaaccgt cttagaatga ttgtcgtaga acgtaacttt tctggtagtg tctatctcaa 240tggcaaccaa ttattagaag gntttttgcn agaatctttg tccaattgca attatntgca 300g 30145766DNAGlycine maxunsure(1)..(766)unsure at all n locations 45attgcactcc gtgtagttgg gtccagaaga agcatttaca gaaacttcca tgtattggct 60ataggcatgt agagcaacgt tgttcatcgc ttcaaatttt ttaatgtagg cttttggtat 120tgggccgctg aagttgttgn aagagacatc aaaaatgact aaacngggga atccatgctt 180gatctttaaa ccggcaatgg gaccgtacaa cttgttggct cgcaaaacca atactttcaa 240ttctggtaga gtttgaagcc aatggggaaa cacatccttt atttgattgt ttccaagatc 300taaaacctcc agatgaatgc aattggacaa agattctggc aaaaaacctt ctaatanttg 360gttgccattg agatccagag ttctgagctg acagtccttt gaaaagatac ttggcaaagt 420gccatgaagc ttgttcagtt gtagatccaa aactagaagg gatnatgagt ttgcaaggca 480ttgtggaatg gttcctgtca acttgttgtg agacaagttg agaatctcaa ttgcacttgc 540attgcaaatt gaggaagaga agtcaccagt gattgagtta aaactaagat caaggtaacc 600gagttgttgg ttccatgaga attggtgcaa tgattgtgtc aataggttat gagagaggtc 660caattcagat aacgatattt catgcaacca atttggcact ctacctttaa agttgttatt 720ggacaaatag agcgattttc agattgggga ctttttcccc ataatt 76646301DNAGlycine maxunsure(1)..(301)unsure at all n locations 46atttccattc aacaaagtga atagtatttt ttacaaatta taattaaata tcaatanaaa 60gattgctata ttacaattat acatcattgt ataatattaa aatatataat aattgaatta 120ttaaaattac atatatatat atatatatat atatatatat atatatatat atatatatat 180atatnttgct gaagcaactt tacctcttca cactttctct atataggtac aggtgttctt 240cgtgtgtttt ataaattgga cttttnttaa gntaccgtac attaaaagtc gttatgatga 300t 30147301DNAGlycine maxunsure(1)..(301)unsure at all n locations 47tttcagtctt tattgttttc ctttattgtt tntttttact actttgattt cttgttttca 60tttcatatac ttttcattta tatttccaac ctttgtcttt tacacaaant cntatcttct 120acattcttct tcattcacct aaacctaatt tcttttagga gtaatttgag aacccatcat 180aatcaagaag catattcccc tttctttgca cnaatcnttt gattattctg gcctttccag 240atcttgttga gactagggaa aacatatttc gtctgatttt gaatttttaa gtgacatnaa 300t 30148533DNAGlycine maxunsure(1)..(533)unsure at all n locations 48attgaaaatg agaccagtat tcattnacaa gctgattgaa tagcgatcct tctttcttca 60tcaggcacat tggatcatca tactccacca attttcctgt caatacaagt atccctttga 120agaaaacaag taatagtaaa aaaatattct tcacctttat tctaatnaaa attcaacagt 180gaaaatcaag tacccaatat tttacaagtt atcactaaat caactaattg tctattataa 240taccctttaa ccaggtgaat gcaattaaat cctctgctaa acaacataac atgcctatct 300atggggtgtg tcaaacccag tggtataggt agaatagttt aacataacca taagaaaatg 360gaattagagn tatgaattag cccgaaaaag gtttgttgaa tgacataaag gagtcggaaa 420tatgcatttt taccatctct gattgaaaga accatagtgc aatccatcac ggttggtatc 480ctgtgtgcta ctgtgatcac tgtacaatct gcaaactcag tcctaatggt ctt 53349301DNAGlycine max 49gttcacaagc gctgttggag gtacatacca aatgtagtaa aagcattaga aagctcaaca 60aacttaacga gctcaacaga cagtggctta aaagttgaaa ctgaagcaag ttgatgcttt 120ctagtgactt tgggtattta ggtcatcgac attataccat gccttagagt tgattttcaa 180tcggcattgt tgttttcgac tatggttgca ttttttagtc gaccatgaaa tgtgctctga 240gggagtaatc tagttggatt tggttctttc tttgtgtttg tctttgaccc atattttcga 300a 30150301DNAGlycine max 50atcatagtag gatcttgaag ttcaactgaa aatagataga gagaaagaga gaatttaccc 60aacaaaagga gaaaaaacag taaaaataaa aaaagaagaa gaagaagcaa aaggaacgtg 120gagtcccgtt tttgttggtt gggtttatga gggaattaga ctgaagtgtg aagccacgct 180agctgctaca attaaattca tagtgatcat tagtcatata ttggatatct atcttgtact 240ttgaaatgac atttcaagaa gctctgaccc tattttctct cacaaagctg ccatcaagat 300c 30151301DNAGlycine maxunsure(1)..(301)unsure at all n locations 51tccacttagt taatccagac tgtcgatgca tttaacaaac aaacaaacat taaaagccat 60aactgaaggc caacactata taaagggtca ggatgcatac tttgcaaagc aagcatagag 120cataggtcat ccaaacaacg actggtgcag cacactgaca gaagatggga acttcagcag 180ggccgaggca ttggcctcaa ctcatctata cgttggcatt ttgcctaatt gctgtcagtg 240ttgtcgctgg tcatgataat ccttactatg cctctccacc atttaatgaa gaactcccnc 300c 30152301DNAGlycine maxunsure(1)..(301)unsure at all n locations 52aattggaata gcgtgcaatc ttattgggtc ttactcaaaa aaataaaatt gggtcttaaa 60tagacgtaag attgatttat aatgcattta atatttttac atttctcata catatanatg 120tgagctttga tgtccaaaag gtgtttcatt ttccaggtca tatatgtgaa ctgtatcgac 180ttgagattga tacatcatac ttcaatttgt ttgaggtttg acgtgttgcg gtgtgaattc 240aacagagtcc taagtggaca taacactgat tcataacgta aagaatttaa tatttttaca 300t 30153301DNAGlycine maxunsure(1)..(301)unsure at all n locations 53caccncaaca caagacaaca aacacccttc ctttaataat agctagtgat gaggtgaatt 60aatttcactg ggcatggttc ataaatacgc attgcttttg gaagaaacta cacaaagtag 120atggctcggg aaatgaagca atagctggat gttcccagcc tctggttgga gtacttttac 180cctcattcat accattattc tcattcttct ctgatgttcg aacttgaata gtgatatctg 240cataaattgg ctgtggagaa agaacgagtg gagataacaa tggtattgcc cttgaagcct 300c 30154492DNAGlycine max 54atattgtggc tgcattgtct ggcatgaatc tgtcagctga tgatgtgtta gatggtgata 60gccatttccc gtcacaggtt gagtcagatg ttgataatca tcagagatac ctatttggca 120tgcaaggtgg tcaggatcct ggcaaacaac atgcatactt aaagaagtct gaatcaggac 180acttgcataa atctgcttac tctgattcag gtaagaatgg tgggagtatg tcagacatca 240acaatccatc tttggatagg catgctgagc tacaaaagtg tgctgttcct cccaataact 300catacttcaa gggatcacct acctcagctt ttagtggtgg aggtggcgtg cctgctcagt 360actcgccctt agatggtact aattcagcat ttacttacta tggcctgagt gggtacgctg 420gaaatccagc attggcatcc ttggtggcta gccaacttgg aactagtaat ctgccaccct 480tatttgaaaa tg 49255586DNAGlycine max 55tagttcattt gaatgcattg aaaaagtaca cctatgggaa gcatattgtt gctcgtgtag 60agaaacttgt tgctgctgga ggtaattatt tcttgcttgc attaccgcta cattctctga 120atatgtgcat gaataggaga tacacacctg aactaccctg ccaattatat gcctttagtt 180gcatacttta ctgtttactt ttgatctgct aaggtattac gttacattac gtgtagtttg 240ccaactgggt catgtaattt gttgcagaga ggagaattgc tgctcagtct cctcatcctg 300cttaggtggg catagaaagt tgtttgtaca gctaactgag gctagtgtga gctttctcct 360ctcttttcct gcatccggag gttatgttta ttcctatctc aatctgtcgt gatgggtagt 420ggccgattgg aaataaaaac actatcggtt ttaatgaaga actgtacatt ttgtagtcca 480aagtttatac atagaagaag aatttaagtg aggcgaggct cagatgtgta atactcacct 540gatgcccccc tgcagcagag gagataaagc gacaatacga gccttt 58656121DNAGlycine max 56aaataaccta catcgtttgg aatattatgt cttaggttac atcaagctca aattaattaa 60atagacctat ttgataaata cgtgtggctt gggcttattt taaaatttat tcattttaaa 120a 12157301DNAGlycine maxunsure(1)..(301)unsure at all n locations 57ttaatgtaac angcataagg gcaaccagcc aagctagaca acctcaacca ggctagctca 60attacttcaa gtcagattaa aaagatataa attggtaggg agaaaaccaa agtttttgga 120attgagtttc aaaaactgta tttagttttc aaaatccaac taaactaggt tgcttagaaa 180actggtggag gggtgtgcaa agcatagagc agaatgtgca attgagggag aggttgaaca 240aaataaaaac atcttctagc tatttcngtt ttatggtctt taaaacactt gtttttttgg 300g 30158895DNAGlycine max 58ttcggactcg tacgggggac ctctaaatcg acctgcagcc gatgtaaaga tcatatccta 60tctcttttta agttttactt gacctagcta accatggcat attttgggca atgacagcgt 120atttattatt atgagctata gatttaagta gtttatagaa cataaatcca aacaatttgc 180tcagatttct aatatatatt acgagattag aagcaagcaa gtctacaatt atcttagcga 240gtgtttggaa ctaattccac aacgcacatc atatctacta gaagcaacaa gaagtaattt 300ctgcatcata tgggcttggt gacgtgattt ttataggacg caattccagt ggaaattctg 360aatatacaag cagtcaattt ttatagcatc taaagcgatt cagtaaagcg caacagcaaa 420acaccagtct tatatcattt atcgcttaat ggtgaagctg aagaactcta aagaaacgca 480atgtgaaaat agaatattta cataaataaa aaggctaaat tgtttctttt gccccctcaa 540atatgagaca agaatcagtt taatcccata acatattgaa gtcaaatcat gacgggtgca 600cagacgatga ccgtctatta ctttccgctc tcctttttct cttggctgga aattgtggcc 660aaaatacaat attttttttc ttgtcaactc gaatggagta agatgggatc aaatcaaatt 720gacaatgttt tgtgggatac aaaataattt tacaagttac aaccacacac gcaagaaaag 780aaaagtgaag aatgggacta aaacgaaact ggcctaatga ggataaaaaa gaacacttca 840gtccaaacca aataataaaa gaagcttcag gttagcaaag ccagtcttga tggca 89559484DNAGlycine max 59tggaaaacga atcaagcgtt gaattggttt gaatttgcat cccaatccaa ccaacgtcgc 60aatttcattc ttttcatcca ataattcaat tcaccaatgg agcttttgaa gcctttgcac 120cctcaccatg cacccatttt gcgaattccc tttcacgctg ttccctcctc ctcttcttct 180tcttctcaat ctaaggttcc ccttcctatg atttctaagg ttcatgtagc tgttgggaaa 240tcgctcgaca

aagctgtccc cttgcttcga tggactttaa atcacttccg gaacgcggaa 300atcgtcattg ttcatgctta tcaaccttct ctcaccatcc ccactctatg taagctcatt 360tcattcaatt ttgattctga attttcatga tctccacttt ttcccccctt ttgcgctaat 420tgattggatg tatttgaatt ttgtgcgttg aacagtgggg aaattgccag catcacaggc 480tagt 484601056DNAGlycine max 60aaagaaatat gttctgcgta agggtcgtga ttcagagtat gcatggcatg ttgtccaaaa 60tcatgcagac aatgcattgt attgtgaaac acctgccagg gctttttaga aagtgaatct 120gggcattttc ttagatgctt gtgattgtct tttaacataa ctgaaaagaa ttgtatatat 180cccttttaac acttttcata ggatccctgc tcacctaacg cttttgatct tttggccata 240atgcattact aacaacactt tcaacattga gtgcccatat taataattga cattcagtat 300ttcccttgct tttccattaa gtacctgata tcacatcctt atttcctctc tccgctttaa 360ttttaaataa ataaatccgt tgcgaaccac ttggataaat gagtataaaa tctggagaaa 420ctaggcttgc atgtagatta ctagatttct cgtttgtggg gttttaaata cgtatgcgta 480agtgatggca atactgctct taaaagtgaa ggctctactg ccgccacctc cttcgtggag 540cagcacaatg cctccgcaat tgctccaaaa aggaagaaat attgaattgc tacttctgaa 600tcgaggacat caaagtcatt acgatatgac aaatgttaaa ttaagaaatt taaagtaaaa 660atatttttat tgggaaatac aatattatat tattcattgt ttttttataa tttatataat 720aaatattttt ttaatttcct aattcatgtc ctaaaaataa tttaataaga tccataagaa 780attagtagtt ttttcttaat tatgaccggt tagtgtaacg acacaatgat tctgtttctc 840tttattgtcg acagagctat agaaaaagct gatgagcatg gctattggct attgcaaatt 900aatgataaag cattctcact cgttatgtca ctctttttga ccccattctt gctctaattg 960gacttggatt ttaccaacag cgtctcttta ttcacagttc ttggcaataa ttctcagttt 1020ctatgcattg cacagaacaa caaggagtgg acgatt 105661301DNAGlycine maxunsure(1)..(301)unsure at all n locations 61aagactgcag catgtcacta caaattttgc tgtttgtaag acgattgaat tgaaattctt 60tcctttattc tggttatctt ttcaactttc ctttgttaag tgtctttgag aactgagctt 120gaggaatttt aattacatag tccggaatca tcatgccatt tactctgatg caaggtgtac 180acacaagtga tgatgtgaaa ttgattcaca atatctagtn gaattttgtt tngaattgta 240taattcanct tgtctggagc tagaactaat ctgtagttag ttttacatga ttattttctg 300c 30162301DNAGlycine max 62tataggatcc aaagctagat cagctgaatg ataagggcag agtcagcaaa agggcatttg 60agaagagaga gaaagcgcaa gacaaacaga aacacacctt gggtaactag tccgaatgga 120gagaacaact tcttgagctg ctcctgtgta gtgtaaagca taatcatgaa aaatgaaatg 180aagcaatggt gagagagggg ttgggcttca atttattgaa gagagggagg agagaagaac 240cctaactgtg gacgaagagc tttgtcccga ttctccgtga catctccact ttccgattga 300t 30163301DNAGlycine maxunsure(1)..(301)unsure at all n locations 63aatatatttt tggtgataaa aatganagtg gaaaatatac aagtatgtca gtcaagttga 60atacggctag tgaataattc aatgaacatg anagatatag gaattattaa agatacaaga 120ataacaatta ataattcacc taatatattt ccgaaaatgt cttacccctc ctaataaatg 180tggctagcta gcaatagaaa atttgtaata caaataagga taaatagtna cttttgtttc 240ttgaaatagt cacttttgtt ttttgatgtg taattcattg ataaatacat ccctaaaagt 300g 30164301DNAGlycine max 64aataaagaaa aacaaaaaaa atattattat agtagtagta taaattagtg tttgaattgt 60tatctgtatg actgtatcat atatttaatt atatccgttc tcattatata agagttatat 120atattgtaat atctccctcc ctccctcgaa agctaaacgc ctacatggcc caactctctt 180cagagtcaac gccacctaaa tctaaacgtt attttctgta gcacacaatc agacagcaac 240cctttcatgt gtgctgcaat cccatgatta aaaattggcg gtcaacggct acgctctcca 300t 30165301DNAGlycine maxunsure(1)..(301)unsure at all n locations 65gncatggctc cctggngtta gaggnggngg caatctcgtc gacccagaat ggcttgatgg 60atcgtgagtc cacccnaatt ccttcccnct ttttggtttc aaaatgtgtt ttctataata 120attccatgca tattcagcaa tccatttgtc atattctgaa aattgtttct aaaattaagt 180gttttgtggt ctgagcttac tggtgtgagt catctgtatt ggtgttgaat atggtcctaa 240cttaagggtg tgagtcattt gaaatgatgt cgaanggtga ggcatgaact tggtttagtc 300t 30166301DNAGlycine maxunsure(1)..(301)unsure at all n locations 66aggtatttat aagtgtggtt tagtcaactt ttgggtatct caaaaaaaca aaagtcaact 60tttggagaaa gagtaagcaa agccggctac atgcaatgca agtgtattga aatgatgata 120tggctaaggg ttcacataca tatgaaaccg ngctcacggg ttttgtcttg tgccgaggca 180cggtctactg tgatgcngct agtgcaaata aaaatgatac cgcgtgagag tgtgtattta 240gtcaaagttt tccattttgg tgaccaccac accaaaaagt agagctttat gcgggaaagc 300t 30167301DNAGlycine maxunsure(1)..(301)unsure at all n locations 67atatggaaca tacccttnct gactcacccc ttatgtgcac aaagcattat gattcaagct 60ttacaaaatg aaagaattct aaatctctgc catttcaaat tccttgcaat tttgaaattt 120ctcatccaaa acacaaggta aagacaaatg tcaaatttta actgttatac cggtacagct 180ggcaactggc acctgggaag agaaataact caagttgaag ctaacaagct ttaatttgtc 240cagaatttca tgatgaaact caaatctttt gttaccaaat tcagtttata tgctgtagtt 300t 30168301DNAGlycine maxunsure(1)..(301)unsure at all n locations 68atttaaacat aaacatggca ttaatgaatt actaacactg gggctcggct atacacaggg 60aaggaaagag aaaaagagag agtagattaa tcgataaaga gaaacaaaaa gaaaagaaaa 120acaacatagt atgtagatac ccaaatagca tgtggaagta agatttagtg gccagctgac 180cacgtgattt ttatattgct atagactata aataagtgcc cactagtgtt tgtataaact 240tatgtattaa tattaantca tgcatggagc tttaatataa gctatcggct gngtttatga 300t 30169301DNAGlycine maxunsure(1)..(301)unsure at all n locations 69tacggccaga ttctgaaagc tgcttcctag aaagtaccta ttgcttttat tcacggtctt 60cataatcact aagtggattt acttcggata ttgtctggta ataattatct accttgggga 120ttttgctgtt gaaagancat catgatatag atttaattaa atagtattgc taattaatgc 180taatgtttgc tttggaccgt acaggagaat ttcaagtagc catcaaaaca gaaccggagt 240gatggtctct ggtttattta taatggaagt tggcggtacg tcaaatttgg cttcttctat 300a 30170301DNAGlycine maxunsure(1)..(301)unsure at all n locations 70ttaacttttt catttttatt taatataaaa tttcacctca aacttataat tcaataatat 60aagcttgtag gcaaggttac ctaagtgaga tnagttcata ttaaaagtgt gaatatattt 120cagtgtgttt gtcggaaatg aataattatt ttcctttcct agttaaccaa ttgctcataa 180caaacttttg ttcgtttggc aaaactcaaa ttgcaacata ttccctcatt ttngttaaaa 240gagaaaattg ccatgaaaca gcttgaaaat tgaatacaca aagggagact atctagtatg 300t 301711102DNAGlycine maxunsure(1)..(1102)unsure at all n locations 71gtttcgccag cttgcactgc ctgagatgaa gtaattgctg ctgcggtgct gctccggcac 60cgccttgtgc tggtgcccgg ccaccaggta gagcaaaaga tgtcactcac tctgttcatg 120aaaaatgggc tcaaggtcaa tgtgcatgag agggatttga gaggggttat cacaagtatt 180aaaaaggaaa gggaggaaga tgttgatttg agaagtaacg aaagttagtg tggtgttcaa 240tagatgaagc agagggtgtt ggaggttttt gaatgtggag acaagttcaa atgagaaaaa 300ttcagaccct ggggctttag cttatagaac aagagaacat aatttccttt taagaaaagg 360gtatattcag agtatttata attcttatga taaattgtgg atggattctt tttccagggt 420cgtgggatgg atgattgctt tctcaatcca tcgttccttg tagaatcctt gcaaatattc 480attgtattct ttatttcttg tcggttttga tgtttctatt taattttact ggtggtgaga 540gctaaactca catttcacaa tgttgaatgt tgatgttcat aaaagaatgc cttacgtttt 600atgaaagtat aatgatcgga tttgactctt ttgtcatata taatggatga tgcttaagtg 660gtagtggtat actcaaaaac tgcaaaattt agctttacag ttcatctgca ttttttggtg 720aatattcatc tgtgatttta gattctgttt ccaggatcct tgcctatgac aatgaaattg 780aaatgcacan attgaaccag tagtaaaagt agatatactg atgtcttttg ttaggggaca 840ttgaatcaga aaactgtgcg accaattttc tcagccatgt atgatgaaga agcagagttg 900gccatataac atgtatttta actttaagca taagtatgct tgagttatat aagtggacat 960tatccaccat ctatacagaa accatttaga tcatgggaca agacatttgc aaaaggtgcc 1020tagttaatcc aagatttcta gataaaatgt aaaggctttc agctatttga tcaaaaactt 1080tgatggttgc tctcttcgat tt 110272301DNAGlycine maxunsure(1)..(301)unsure at all n locations 72tattttaacc aattagcata agagacaata aaaaataact ccataatatt aacaagattg 60tgacttaagt tgcctatcca caatttgnaa aataatttat caaaatgcaa gcacaaaaat 120aaatttacga aattgaacaa aatgcaattt ttaaggaaat aaacattgaa atgctatcct 180tggattagtt ttaaggctct agtggtacca taacttgatg tgccgcacgc tcacgtattc 240tacaattcgg ataagaggta tagactatag agaaatattg tctgattttt agtttctctc 300a 30173301DNAGlycine maxunsure(1)..(301)unsure at all n locations 73aaatcacact ttactctcaa gtgcactttt gcactttata atatccaaat tnatatataa 60tttttccccc acttagaaga gacgttaaat gatgtcagtg ttgtcaactg ggtactctaa 120ctttaatatt agtctgtttg gatataagtc ataagctctt ttgagagttt ctctactaaa 180aatataccat tttcttgttc tcatgccttc ttcatgcttg aacttgaaca tccctttaac 240aaatgcagga agaagtgcta gttaatgcct ccgtggtgaa aagtggaaga aaattgactg 300t 30174301DNAGlycine maxunsure(1)..(301)unsure at all n locations 74tttgcttnca ttttacttag ttattacttg agattttcta tgaaaatgca tgttttaaca 60agccgatgat ttcttcaatt gcttagcaga tatantnatt gtcataatgt taagagtagc 120tacatctttt caacaangtt aaaatgcact actaagcatt taaacacaca ttagccaaca 180agtccatttt ctgcaatgtc ttttccaatt taaatgactt ccaaaacata tgcactcatg 240ctccaaatca agtaaaaata tcttcaactt tttttcatgt atatacagac agagaaacac 300c 30175301DNAGlycine maxunsure(1)..(301)unsure at all n locations 75tagtgaaata cgtaatgcgt tagttgaaga gaacaaaagn ttacatgttc anatatcaaa 60agtctaacaa tttggctaca tgcccaacac gttatgcant ggagatgagt gtactataaa 120gggaaggcca atgtatgcag gtgtattcaa tcaatgtttg tcaaatctgg tgagctaaac 180tgatgttggg naaattttan agaaggaggt tgattttgtg gttggaaaag aaaggtttga 240aggacggagt gagttcaaat tttatcacta atattttaat aaaaattaat aattaaagtt 300g 30176301DNAGlycine maxunsure(1)..(301)unsure at all n locations 76ttaaaataat taatatttta tgtatgcaac cttanttaac ccccaatgtg tttctttcct 60tgggtgtgac tcgacctaag tcagtgagtc actngactaa ntgcaaacnn gattgggtta 120cactaagttt aacgcttnat caatctgata ggcaactcga ctcagtcaaa cttttgagtc 180gcagtcaaac cagttcaatc agttgggttg gtctngattt taaaacatta tcaccaccat 240cactattgtc attactacta tttcaccccn accactattg tcattgctag caccaccatt 300c 30177301DNAGlycine maxunsure(1)..(301)unsure at all n locations 77tcttcataaa aaagacatcc aaanaataaa ttctagatga tagaaattta aattntatga 60taaattattt tncttaatta aaattcttta tccaaataaa ntctagatat tttngggaga 120aaggaaatga tttatttcta ggatagagat agtgaagatg gtcctcccac atgtgattct 180aatttgggct catatgtagc ttttagaatc cacgtcttat ccttttgtcc attccatctt 240gctacttctt cctcttgaac tgacaaataa cattcttaaa gagcagcata attcttcaaa 300c 30178301DNAGlycine maxunsure(1)..(301)unsure at all n locations 78aggcaactta actaacaaaa acaaatttct aaagtaaaat cagaaaacac aggtagcaat 60tctgagctta accataataa aaacaaggaa taaataaatg gagtcattta cgaaaattcc 120tttattgtta atatgaaaaa aagaaaataa atttctgata gaaacaagtt actaatacta 180tgaacagnaa caaatgtgag aacacaagca cacagtacac acatggcatg tgcgcataca 240tttgccaatg tgagcttata gccttctact ttacaggcac atcttcctag agacacatgc 300a 30179301DNAGlycine maxunsure(1)..(301)unsure at all n locations 79gtggatgcca tgtcaaaaga aggtgtttca aggatgaatt ttaaaatttt catattcctc 60ttaaccacat gaattataag attccctcta cgggtctgaa ctanaaantt tttcatattn 120cttgggttga ataaacctga ctacatttaa aaacagccat cacgagccaa cccttcgcta 180tgagcctact actgtaataa ttaagaggat tttccaaaca aaaactaaaa attttcacgt 240tcaacaaagc aagctagcta acttgaattt aaattgatgc agtgtaacct tctataaact 300a 30180301DNAGlycine max 80aagtgcattc ccctcatatt tctatcgacg tgggactcca tgataggtaa aatgatttaa 60taactgataa ttcaagcctc agtgcaggac atgagggtat ctatcgatgt gagacttatt 120tagagaactg ccattgttaa actgatgata tagatgtggg actacttctt atgcctcaac 180atgatgtggg agtgggacta ttgctagctt aatatatgct tagtttaaat cactactcac 240aattaaaata gtccttgcaa tattcaatat tttagctttg gtccttgttg ttacttaatt 300t 30181301DNAGlycine max 81caatatggca tgtttatctg gtaagcgtag ataattagtc tgaaactgac actcaaaaaa 60ctttaagcca acgttatgtg aaccagtcaa atcagtatca taaaaaaaaa cagtcaaatc 120agtagggacc aatcctagca aattagacca ccaaaaggga ttaattacaa aggagaaatg 180attttcttgt aattaatatt ccaactaata tggcattgaa caggaagcta gagaaacatc 240ctaatgagga aaatagtagg gcaaaacaaa ataaaaataa tgcccttctc tataacagaa 300t 30182301DNAGlycine maxunsure(1)..(301)unsure at all n locations 82actttgtcaa aaagaaaagc tttcccatgc ctgcattgga aagactaaga gaaaagnagg 60gttagcttgt gtgcaagtta caaattttac tcatacattt agtttgcatg atgagctgta 120caattaaggg ttcagataca tattggcaag tggcaacaaa gggaagttgg aagtgaaaca 180gctactattc tcatcgttcc taaaatgttt ctttcattta acaaaatgaa tttgcaagag 240taaacatatc acagaaggca ttaatcaggt gccataatca gggcagagaa taattttaat 300g 30183301DNAGlycine maxunsure(1)..(301)unsure at all n locations 83aggtttttgg gcttagcgca cangtgtgcg ctgagcgagt tatgcaactc ttattggcct 60gcaactttcg ttaagtagga catggctcac ttatcgaatt aaatgcctca tgatgcagta 120gaggggtcac gcttagcgag atgggctcgc ttagcgctat gccattttag agagagttat 180gggcttagcg ggtatggtac acttagtcca atagccatga aaatccaaga agagagcctt 240ctacgcttat cgcatagaca cgcttagcga gagactatgt cgcgtttagc cctattccat 300t 30184301DNAGlycine maxunsure(1)..(301)unsure at all n locations 84catantctta attcgaatgc tattttcttc tttgaaacgt tgntttattg taagagaatt 60gaggaaataa aataaagtta ggttttgatt attttttntt gttgattgtt caggaagaaa 120tgatggttta actttttttc tgtaggaaac ctttccactc tcaacttaat aggtactcta 180gacaagccaa cttggagact aattggtttt tatggattcc cngagaaaaa taggaggaag 240gattcttgng attttattga gaactcttgc ataggatcat tctctcccnt ggtgtataat 300a 30185301DNAGlycine maxunsure(1)..(301)unsure at all n locations 85ttggcaagga cctattttga gtctctctat tctcgtgatg acggggacca tgcaaaacta 60ttttnttttt tttattcttc agtaaatatt agtttgaact tttcatgttt ttacaattta 120ttataaaatc taattattta tataattaga gtaaaagtat tttcgtgtat gtttcacgtg 180tgtgtagtga atcaaaacta acataattct acatagatag atagatagat aattttttta 240cagaagtata cacatattta atctgctatt aatttatgta aagaatgttg cataaaagaa 300t 30186301DNAGlycine maxunsure(1)..(301)unsure at all n locations 86catatcctgc aagataagat agaactggaa atcttctttt tcaataaata gcgatcaaac 60aatgaactta aaaatccttt agtcttgttt atcttagatt tatgattaag ttagtgaagt 120gttttaagat aaaatatcaa acttattgga attgatattt ttttgggtat ttaattgagc 180atttttacaa gacaacgatg ttcgggtacg cccaatgtgg tagttaagtt cttgaatttt 240tggcagataa ttaatcatat acacngtcaa taaaaaacat ccttatttca aaaatatatg 300t 30187301DNAGlycine maxunsure(1)..(301)unsure at all n locations 87tagaactctt tttcttattt gtcttgatca aagaatgaaa cccaatatat gtatataggt 60aaatgctgtg gcataacata agaaattaaa gtgaaaaaaa taaaacggta atttcatttt 120caacgtaatg ctttattaaa cagaaaagaa gagaaccccn atctcctcaa ttatatgatt 180aggattaggg cttctgcgtg tgtaattatc attcgccaaa aatgacatct ttgttaccca 240gctattgcgg gaaatataga tatattactt atgagaaatt aaagctgcgt acattactca 300a 30188301DNAGlycine maxunsure(1)..(301)unsure at all n locations 88gcgctggttn tcttcgcttg tacaaacgaa agtaattgtg attactataa aataggcaaa 60taacaaatgg tcagtgatgg cctcaaactg ggatgcttgt caatttttgg ggcagggtct 120ttcacatttg atgaaccttg ttggctatcg agcagggtat ttccaaagtt tggtctgcaa 180aacagattac tgagaaacca tcaattngac caaatattta gatgtctatc ttcaagctca 240aggagcgatc ataacagata tcaaatgtct tgaaactcat gggagttggt tactgatcac 300g 30189301DNAGlycine maxunsure(1)..(301)unsure at all n locations 89tccttggtgc taccgtatac cattttgtat tttcaattta cagttgtcac atgttacata 60aaattctgtt cctcttaaaa aattatttgt gtactgttgg tctattaaat tgtcatagta 120tgtatgtatg atagttctcc taacaagaga tgcaaacagg ctagtaaaga tgcagcgagg 180aggctgaaaa ttttgggcct agtcatcaca gtttagtcat gtttcactct tgggatgtag 240tagtattaat taaaatgttc ttataagact tgaacaatcn ttcttaattc aaataaaaat 300a 30190301DNAGlycine maxunsure(1)..(301)unsure at all n locations 90ttcccctatt ttttatgtag aaaaaatgta aatatgcact aaaaatttta ctattttcat 60taaaaaaatt gataatttta attttaaact ttatacattt ttagtgcaca atgccactat 120aaagtttgnt tttttttccg tcaagggtgg ctgaaggcca tacgaacaga acgggggaat 180gaaaccccta aaaaaagttt taatctcaaa cttttacaac tagaaccacc ctaataggtt 240atttaaatta ataaaatata ataaagttaa tatgaattgg taaacactaa ttaatgaatt 300g 30191301DNAGlycine maxunsure(1)..(301)unsure at all n locations 91gacacttgat cgttaataga nanatatata tatatatata tatatatnna tagagagaga 60gagagagaga gacttttttg agtaacttga aagggtgaca atgtaaaact ataagaggat 120aatgtaccca aattataatt acaaaaggga gaaaatgaag atcacatcct tgaccaaagg 180ggctatatac tgatggcacc atagcaatac aacatggtga taaaacatga cattttcaac 240aaggcttctg aaattctaaa gaagtatttt cacactaaaa gaacaattgc gaatatttac 300t 30192301DNAGlycine

maxunsure(1)..(301)unsure at all n locations 92aaggagttga gctctggtaa ttaacgaatg caacacaana caagtgagtg aagagaaaaa 60acggggttgg gttgggtgcg attctgaaan tgacggaaac gtcccattca cgccaacaca 120ctcatnatta accaactaac ttcaactaag aaagtgaaac ctctcccctc cttacctttg 180cttttaccaa tgtttccttc cccaaccaaa caaccacaaa tcttgtctcc ttacttccca 240tttctttttc tctttttggt tttattcttt acaatatatt aatttaattt taatcttttc 300a 30193301DNAGlycine maxunsure(1)..(301)unsure at all n locations 93ttactcaaat tttctcctcc tttagttagt tgaatncttt ctaggctgat tggtgattcg 60gattcattat tagaggctcc acattggatg acagaggcct ataacacatg ttgaggttgg 120ttcgaggtgg tgaatacata aaacatgatg gaatttgata actttaagtg gtaggggttt 180ctcattggat gaaagaggcc tataccactt gttgngattg attgaaaatg gtggatacat 240ataatgttgg aaaatttgag aattgtcatc ggtaagggat gattgatttt gcaaaagatt 300g 30194301DNAGlycine maxunsure(1)..(301)unsure at all n locations 94ttgcaatctg actcaaacaa tatgttgtta atattgagct ccctcctgca gcagacccat 60tgctcgaata aattggccat ggtcatcacg taagtaagat tcctattcca aagcactgct 120gctgttaaaa tatgttagca tcttgcgttt aatttcacct tttggagtgc tgcatttgct 180gttgttggaa gtggcattat atatgattcg aatcatatgt tttgttcaat attttttatg 240aaattaatct tntaaagtga ataaatattt ttaaataaaa atatatttac cattaattta 300a 30195301DNAGlycine maxunsure(1)..(301)unsure at all n locations 95ctgatattag tggttactat cgacagccga caggcaaagt atcatcgatc cacaaccaac 60tgaagaaaat aaaattagtt ttttttttat tatttacata agtaacaagg gaataacgat 120aaaaagtaaa aaaaatataa aagaaacttt ccactacata gcctataatc tagtgtcatt 180tttgggctct ctttccccac atctatcaac aacattgacc atattaaaac atantgttgc 240aatgatcgaa aatgattttt ttcacaataa ctaaaaataa aagcaactat accttttcat 300a 30196301DNAGlycine max 96aaatagaaca aaatgtttta gagaattcag aatgagaatg ctttcttatt caagagaaca 60tgaaatccat ctaggttaat attaattcag cccaacttca taaatactaa ttcccacgaa 120cactaaatgc atcttatcat gtgatacata tactattacc tagtgcattg actcttataa 180tttccagcag aaacagcctc accgattaaa ttcaaaatga tgctggtgaa gtaatagacc 240taaaaattac taaggtaaag aaaaatacgt aaaacagacc agaaaaataa aatacaaaga 300a 30197301DNAGlycine max 97gcattaatga aaaatggagt acacggaaaa gatcacacgc aaacaatatt ttgtcaagga 60aaacgccata cctatacata cacagaaaat tcatatttga cagggatgga gaaaaacata 120tctgtaccta cacaagggat agacaggtaa tcttccccca agcttcctcc ttcttcaagt 180ctttttctct attatccggt atttcttagc cgggaaggca ggatacctgt caaaagtatc 240ccaatgctca agttatatct cagacaaaaa aaatagaatg cgtacttaat gtgatgaaca 300a 30198301DNAGlycine maxunsure(1)..(301)unsure at all n locations 98acgattattt atcttttctt tgggaacaag tccctttaaa tatttaaatt tctaatgtaa 60aaaggtccct aatcaancat ttgtttttaa tttttatagt aatttagcat agaaantcaa 120tttcacaatt gctaggaagt tcttttaagt gaattaggaa cccaatngaa ggacaaaagc 180ctccctcaaa tcaattgcta agagagaatc atctgtggtt aatccgttgc taccgctgaa 240cagacgcaaa attcacacca acaaattcat acaaaaaaat atgaaagtaa agagacacaa 300c 30199495DNAGlycine max 99ggagttatat ttcttgtttt aagagcacca aacaaagaaa ctggattctc cacacacgac 60aatcttgttg atgtttcctt tgaaaaaata tctaacaagc gttcattttc ataccaatag 120gcagccatac tgaactttcc tctcagaatt atactttcca ttttcatacc atgggcagtc 180atttgtttgg caatggtggt aacttgctct tcctttgttc cttcatcaga ttcttttgat 240ggacataggg catttgtaat aagacagaga cgtttctttc ccttgtttgt cattccaaat 300ttttttatta acatatccac gccaacaatg acaacatcaa gaactaacat caaagtcaag 360gtgatatgat cagggataaa aacaagaaca atttagtaat tcctagcatt cgaggtctag 420gaacctccag agaagaagct aagaaaggga agttttattt tctgcttgct tcatcacgat 480acatcattca ttctt 495100301DNAGlycine maxunsure(1)..(301)unsure at all n locations 100aaaactccga taancattan aaccggcgat aaaaatgagt gatcaaaact acacattaaa 60ngcacttttt tttaataata aaaatgttag gaagaatgta cgtgatagta gtaaggaaga 120aacaagatag ggctagcttc ggatgtacgg ccaagagtgg aatcttgttt tgcagctcta 180tgaccagttt ggtttattca tcacaggaag gagaaatgga agatggaaac acttgctatg 240gcatgctacg tgttggtgta tgtggaatgc taggaacaac atcgatatca tttgtccgga 300c 301101301DNAGlycine max 101ttaatattta atagattttg aatgagaaat tgattgctga gtactgtgag attgttttag 60aaaacaattt ttaatattta taaccaaaaa atgagaatgg aatcaaatag gccctaaatc 120ttttacaact taatgaggac tatacaatcc acttgtttcc tcatgtttca tgtcgctgaa 180gcatgtgacc aacttggtta ctgtgtaaaa ctcacttcac aaaacatgtc atttatccaa 240atatagagga agatgttgaa cgctaatgtt tgtacagaaa ctgattctca aacttccttt 300c 301102301DNAGlycine maxunsure(1)..(301)unsure at all n locations 102actgctcttt tttcccctac ctacctgcat acgatgttat ctacttctac tttacaagct 60tcattgatct cacttcaact gcttcaattc agatggtaat aactaataac tctcgatcga 120gagccaaata atgttttgag ttcagtttca ccatgcacca tgcccatctg ttttcgtaat 180ttgatggatc taagaagcaa atttgctctg ttttttactg tatacgtgac agagaagtaa 240acacgcactt gatccaattc ctttcgtgga aatcaacagt antagcaaca ataaaacata 300t 301103301DNAGlycine maxunsure(1)..(301)unsure at all n locations 103ttaaattatt aagaaattta tctttttttt tcataaaagt agttctaaaa aagcctatcc 60aaatatcttt gttggtgttt ttactgacaa aggaatgcga agttttggtt gctttggatc 120aattgaagta ataagccggg aagcttggtt tggaaagatc atgtgtttat cggacctctt 180gtttgaaaac atctcatgcc aaatccaatt cacattgcag gcttagcgtg aaggagtcca 240aaccacaatg gtgtttcgtt ttttgtcatg cttatcgctt gtttttgtat tggntttatt 300t 301104301DNAGlycine maxunsure(1)..(301)unsure at all n locations 104ctaaaaagat ctaaattatc atttcttaaa atatctcaat taattcatta ccatcatgat 60tgacgttatc attaccatga gcatccttgc attcatcacc ataaatcatc attgtcatca 120cctctgtcac catcaccttt ttgtcatcat cgtcgttgtc atcaactgtc accatcatta 180tcgttgctat cgtcattacc accatcacaa caacaatgac aagatcatga cggtggagnt 240gttgatcgac ttacaaaatt ataagaatat ctttctaaaa ataactactt ataaactttt 300t 301105301DNAGlycine max 105tttatacact taacatataa ccaataaact cttatatatg taataaattt ttatttatga 60caaatattta atataattat atatttataa tttaaatttg agttcacaag ttatattaac 120tatcggttaa ttaaattaaa atcattttac actggttaat gcacacacta acaataataa 180agttgataat cagatttttt ttttcttaaa aaaaaagaac gacaatatgt tgcctccagt 240ccatggaaat gtggaattct tgatttgatt ggaagataac aaataaaata tgagcaatga 300a 301106301DNAGlycine max 106aaaatggccc aaatctcctt attttgcgaa ttaaataaca tcatctatga tttagcaaat 60aaagaatgtg attttttata tatttttaat ggattaaata atgtgattta tctcctaaat 120attattatta ttattattat tattattatt attttatgat agggttgggt actcacatac 180ccataaccac catcgttttc attaaaattt gtggataatt agtgtacctc agctcataat 240ccattaagtg gataattact ttatttatga tggataattt ttttgagtaa tagtagaatt 300t 301107301DNAGlycine maxunsure(1)..(301)unsure at all n locations 107ttttctaaat tataaccaca gaagtgtcta atgcaaacaa ttngatttgc attattcttt 60ttatttattt atttattaat atttgcatta ttcatatcaa ctacttctta gcataggtgt 120caagataaat taaagtgttg gcgttgccaa cttcaagcag agaaagatca agtggccttg 180actgtaactt tttgactgga tcatcaagtc tattattttg ggaatttatt gaaatatact 240gataatataa ataattttta tattaccaat taattagagt ttgttatatt attataaaat 300t 301108301DNAGlycine max 108aatttgcagt tagtgctaca agagtgagct tcacgatgac gaggagtctc tcccatattt 60gtcgttgaat atttgtgaat tgcggatcac cacacgacca tttccttccc tctttgttgc 120cttcttgaca tcttttgttg ctgcaacact catttttttt caattaacct tttcttcctc 180aacgaagttc aattcttctt tcttttgcac gtaagttgga caattcaatt caattatctt 240cgtagcgggt tttcttgtgt ttcaaactgg ctaggtggtg gttcttgtaa aaagctagct 300t 301109301DNAGlycine maxunsure(1)..(301)unsure at all n locations 109gtattattag canaatgcct ctatgcaaaa cctttggtct tatgaattgt gttatgtttc 60acttcaacga acaagaaaaa cactcattat tctccagacc agtaaaagta acaatggtct 120caattctcaa actcagcaag aaacgcttca aacgtcctag aaagcaagcc attatgacca 180tcataatcaa accaaaaagg ttacaagtgt atcagctttt gaacagagtc cactcggctc 240aaagtgaaga aattttaaac acttacacgt cctctgcact tttttcgact tttnaatcca 300a 301110301DNAGlycine max 110gaagaaaact tacaaagtga aaaatatatt tcaaaaagtt attatttcta cattattaga 60gttatttatt tagaggccat tcgaatgaga tgttttgcaa acaagcgttg gagaaaatag 120agtcgactat gtatttgagc aaaaaactat aagcagtact ttcttttacc aaatgctcat 180agcaaaagga aactgtgggg aaatgattct tggggttagt ggttacaaaa ataagtaaat 240aactgccctg gtgcagttgt aaatctaaca agagatatct ctaaatgaag agtttcatta 300t 301111301DNAGlycine maxunsure(1)..(301)unsure at all n locations 111aaaataaatc ctatgaataa taacttattt gctgttgctt ttgtaatgtt ataaaagctt 60ttttctttcg gacgcttttt ttatttgccc tttttgcaca gcacttgcca atttatggaa 120tagcccgtga gatctgtaat tttctgtatc ccttccccac attggagaac taggctaatt 180tatatccatc tcttagactt tgtacgaaat cgaaatgcaa ataaataatg agaaatcata 240gatgcagagc ctactaantg tatatattag taattattac tatagtgcca acaaaagcaa 300a 301112301DNAGlycine max 112attatttgta agggctggat cctttgaata attactccta taaagactgg gctgatctta 60tataagaaat atgttaaatt ataaatttct acaagtatta ctgcaatttt atgagaattt 120gtttttctat tgtaaattgc aatatattcc cccggatccg gaatagcctc atattgactc 180caaatagtca tgacaaatga agattgaggc gggatttggc atttttaaac cactgctagt 240gcttggtttc cattttggtt gcaagtgtaa ccaaccagct attaaagata caattggaag 300a 301113301DNAGlycine maxunsure(1)..(301)unsure at all n locations 113tctcctcgat gagatccagc ttctcccttt cctccacggt ctgaggcagt ctccctactg 60agaagtgcag aggttgcagt gggcggtgtt gatgagctcc catggcattg ggctgaggca 120taccatcaaa tgtcggtccc tcancggtga atgtggagta tagttcaaag tcacccacaa 180catgatctcg agggtcttca tagggttccc ccataggctg agggatgggt gtacgtccca 240antggggttg ctggccctca aanggaatgg gaanggagtg gttggcgttc tcggtgggca 300t 301114301DNAGlycine maxunsure(1)..(301)unsure at all n locations 114ctcctcgagg ccaccatcat tnggatcaaa atattccggt ctacctatcc tagaagttat 60tgtcctggcc tcccatccac gtgcgtgttt tttcaagcnc caaacaacac cttgtgttgg 120tgcttcttgg cagngcatcc gaccnncttt tcggctgccn tttctagcga cagactcacc 180ggaccgaggc gcacaaagtg tctcacctta ttttgggcca ttctgagatt ctttcctttg 240ccgtacctta ttttagttca cgttgtttgg tcattgtttt gcccttgttt tcaagttcga 300c 301115301DNAGlycine maxunsure(1)..(301)unsure at all n locations 115tgctatggta gatcagtaag gaaccaagta ntattnacct ctaatgcttt aatctttgca 60ttcatagctt caatccagtt aggatcttta gaggcnacan tgtatgattg aggttcaact 120tgttgtgtaa caaacaagat aaccttttga taggaagtag atagtctgtt ataagaaaga 180acagaacaga gaggataaag agtagtacct gtagagagat tggcaacaat tgatgatgga 240tatgtgatgt gaaagtcttt tatacttgct tggcctttgt ctctgtctca ttggccttct 300a 301116301DNAGlycine max 116acttgtaact aactaagcta actctagtta caatgtgatc aatctgaatt aaccattatc 60aaaacagtat ctatgctaag acatagcttg ccttaattgt gacctctttg gaggttgcct 120taattgcagc agcctagagg gggtgtgttt aggccacatt gcctacaaat atggcctaga 180tagtgcttac aaggcttggg taatcctcac attacaatgc ggtgttttgg agctgagata 240ggttctaagc tcaaattcta agagattttg gtggcctgaa gactgaacaa cattttctgc 300t 301117301DNAGlycine maxunsure(1)..(301)unsure at all n locations 117ttatttgttt cttccttttg ctgctgactt ataattgaag gagtttctgc tgtgtttgtg 60tcatcccgcg aaaagagagc ctcacatttc tgggctaatt tctttttgcc cccncctttt 120cgggaataca ccagccagga gttatttggg catggtgtgt ggccttttgg gcttgctttt 180tggggagcaa atgtttttgg aggggtgtaa atatcacagc ccaagtccac taggctttcc 240acctccttgt cacgtgcctt ttgtctccca tatttaaaac tgccgagctc ttcaacgttc 300a 301118301DNAGlycine maxunsure(1)..(301)unsure at all n locations 118aaaaantaaa ttctaaaatg gttttttgaa aaatcgtctt agaatgcata tccttttaag 60atggttttta acaacgaacc gtcttagaaa agtatcattg taagacagtt tttaccaaag 120aaccgtctta gaatgatatc tttttttcta agacggttac ttaaaaacta tcgtaaaaag 180taaagacttt ttataatgtt atctacgacg aaggttaata atcgtcgtca aaggtctctt 240ttaaccgaca taaaaagcgc tttgtgtaac agtgcatatc cataatttat acccgtttat 300a 301119301DNAGlycine maxunsure(1)..(301)unsure at all n locations 119gtcccttgtc attgcctaat aaattaaggt ggcttcattg ggatcaatgc tttcttaagt 60cttttctaac taacttttgt gttgaacaac ttgtagttct ttggatgant ggttgcaagc 120ttaaaaagct ttgggatggg gttcaggtat gcatgttgct atacataata caattttctt 180tcaatttttt atggcatggg tacaagagag agtcctaagc ggtaattggt ttcatgttgc 240ctcaagtgaa gctcattcct aagagcaagg gctacactta ggatgttttg cttctattta 300t 301120301DNAGlycine maxunsure(1)..(301)unsure at all n locations 120ataaatgaaa aaaactaatt attattgtat taaaaaatta aaataataat tatctttaga 60taagtttttt nttctcacac aacaattatt ataggacaaa gactgtactt gtagggttat 120gcaaacgagt ctgacttcct agtttaaaaa atgaggttgg ccttggaacc tctgaggtta 180gaagcctccc caagtctcga agtgcatgac aaatcaagga gtggccttga atgtataccc 240ttactaggca ttgttaaacc ctagggaagc gtgataaagg ggaagggaaa tggttaggag 300a 301121301DNAGlycine maxunsure(1)..(301)unsure at all n locations 121gaganaggct agcgcttgtc tggcttgtgc ctcttgtccg gcttgtgcaa gtcagccttg 60gngtttattt tgcacttgtc acgcttctat ctggcttagt ggacttcatt ctgtaatctn 120gacaatgctt ccatgtggat gtagcccttg gctcactcgt gcagctagtc catgcaattg 180agtggtcttt tgcacaaact atctgtgaac ttgtcgagga ataatgtgag gagcattgaa 240tggaacacta cctcggggta agatttcgga tctggacggt tgtgcgccca aatctgccca 300t 301122301DNAGlycine max 122caataaatat aagattgact taattaatga taaagaaaaa aaaaagaaaa aagttatgaa 60tttaaattct ccacaaataa aaattaataa ttaactttta tggatctaaa aaaaattacc 120tcatagactg tgccatcacc caactgaaca agggtaaaaa gctttctgtc tgtagtgctt 180gcagccacag aaagtaacca tggagcataa acagacattg tagaaagctc tggaccctca 240ttaccagcag aatgggacgt taatattcct ttcttcattg catggaaagc cccaatggcg 300a 301123301DNAGlycine maxunsure(1)..(301)unsure at all n locations 123ggggtatgcg aggctacnga gaggggaagt agggaaaaat gatgaaggct aggatgtgga 60cggctccgga gacctttgcg gtgctctagg agtttctcct cctgaagatg ggccaagtcg 120gtggcatgca ccagcgtcaa cggctanaga gcttggaatt cangccggat gtccgattcg 180aggcctgaga tgaagcaacn tagaagtgat gatggtgaca acccaacaat gcgattggcg 240agtgcttcaa attgagccag gtattctctc antgaatctt tctgggttaa tttaaagagg 300a 301124301DNAGlycine maxunsure(1)..(301)unsure at all n locations 124aggtatcgtg gcctgttgat tctccttttt gtcccttgtt cttcttcttc atttggttct 60aatattgatt ctgagttttg ctttgtaatt gttgactctg ttttcataac acctaattaa 120attaaatata ctcttatgcg acttttggtg tacaaaattc atcatgagct ccttcgtcat 180ttcaagggct cttcgaaagc tnacaagcgt cttattctca ttggtatcta tctccatctc 240tctctttctc tctctctctc tctctcaata tatatattaa aataaaagaa aaattgccat 300a 301125477DNAGlycine maxunsure(1)..(477)unsure at all n locations 125accttgcaac atataagaca gatagcacaa gaatcaagtg gagaaattca gtatggtggc 60ggacaccaac ttgctgtttt aagaacattt agccacagac tttgcaggta attaaggcag 120actttaatgt ccttggtagt tcgaaccaat gcattgtgca ttttagttat tgacttggca 180ttttaaactt gatgtaatta agtagtagtt tgttcattaa ttattctnat tcttgctaaa 240attcaagctt gtncattacc attgcttcat aattactatg ttttattgaa attgcattcc 300ttaggggctt taatgatgtt gtgaatgggt ttgttgatga tggctggtca ctgatggggt 360ggaagatgta actatagcca aaaactcatc tccaaacaaa tttttgggat ccaattacaa 420tgcatcaatg tttccagcat ttgggggtgg ggtcttgtgt gtcaaggcat caatgct 477126301DNAGlycine max 126ttttacttaa catggtatct agagtctaac tgagggaata atgaaaatca tgccccacca 60agaacctaca agatagtagg tccccgatgg atctttctac tcatctccct tcttttggca 120acttgtttct gcattccgat ggaccctttt tgctcgttgt tgaacaacaa ccttttcatg 180cttttctggc caccatcgtt tgtcattatt gaaacttcag caacctatcc agactctaac 240catcaaatct ggtgcacctt tggccgacct gtctagattc aaccaccgca agtcaattgg 300a 301127902DNAGlycine max 127atggacaagt ttaatctcac cttttacaat ggtttgtgat gacattagga aagacctaca 60gtaacatgtc ctgtatttac tgtgtattat tttaaattct tgatatccgg ccctcatatt 120agaagggatc cttacggaag gaaagtttta tgtggtcttg ctcgtaggcc aagtaaacgc 180aaaaacgtat gaaagatcaa gaaaggaaat ccagaaaata tgtcatttga taaacctaat 240tgaacccatt ttctatattt tcctttcctg cccaaatatt tatgtataag cttccttcaa 300cttgtctaca tctctgtcac tttatgactg ccctttatag catctccaat aatgaacatc 360atttagtttt tttaactctt ttaatggctc ttaccatccc acatcggttt taagaacttt 420agcaactttt cactccaatg atgcatctct gtaagaactt aaacaggtct caagtttttt 480acatcttaat ttttctttaa tgggtctcag acgttacctt tcaattgttt tttaatggtt 540aagagtcagt

tcttttataa gaacagttct tactcgtaat gtccatatca tcttcctcca 600gtggtgaacc tagttaaaaa tggttcttaa ctttaagaac ctacacaaga actatcttag 660agatgctctt gtagtgaagg tataggttgt tctaagatgg tcacatcaat cacagatcac 720cttatgtgtt ctatatttag aatttacctt gtctgttaca tcttaaagac gatactgaat 780tactgatgct tatgcagttt gaggatgatg tgtacttgcc aacggatgag ccacatgggt 840caattcaagc tagtatgagc cgcttgtgtc aaagctgtag aaactgcagg catacaaggt 900tg 902128301DNAGlycine maxunsure(1)..(301)unsure at all n locations 128tagtatgact aagattaaaa aaaaaaagaa gtcagtggaa gatttttcaa attgaaataa 60aaaaaatacg aatcttctac tccttaaaga atttgggaaa aggggtagag tgattataca 120tgcatttgaa ataaaaaaac acacacgcca aagagcccgt taatattggc cacagggtgg 180tgttgacgag ttataactat atgccttgaa ggaaagaagt tttttttttn aaaaaaaaaa 240aaaaaaaagc caaatggtat tataaacaac aaacaaagca caagtagtgc ctaatacaag 300a 301129301DNAGlycine maxunsure(1)..(301)unsure at all n locations 129aacacaacat gaactattta tacttcacat ttgctcacgc atttcatcaa ataagttgta 60ggcaagttgg ttaaatactt aaatcccaaa agtcctaaca accagcccaa cacaaggcat 120caactaccat tcaacatcat gtaggacaag tgaacaaagc acagtagatt aggtgggaga 180aggacatacc tacggacacg acagagcgtg acaaataacc accaggacat gatgatgagt 240gtgcattttc agtttgcgag gtggaccttg cgncggagag tgttttggca ggctggagaa 300g 301130301DNAGlycine maxunsure(1)..(301)unsure at all n locations 130aattgatgct tctaagttct aatcgcactt caacacngaa atttaggagt gacgagaaat 60agagccctac tttctttctt gccgcgtttt atataataat ttgggttgtg aaagttgggc 120ttggacngng gaacacagat gncccacaac aaatgggccc taatggagaa agaaaatgtg 180gcccaggtca ctttaaaaaa aggaaaagga aaaggaatac tgccaatcaa aataaaataa 240aataaagtan attgatanca tgggctaaca tgtcttgccc cattacaagt cctttttttt 300t 301131301DNAGlycine max 131taatgctctt aggcgtattt ttcgtttatg ctacttttat aacatcgaac atttttattt 60tcttttagga gaaagaaaga aataaggtcg ggatatgcct ttggctgtct gtttgtaagc 120atgcatgcat acatccggcc ggtgctggag aatatttgct tatcaccatg gcacaaacgc 180attattcatt attttcccct ttttaccaac ttaaaacagt ttcttatatt atcgagtttc 240atagatataa tcatttcagt taagcttaaa aaattggaga atctctataa tttaaattga 300t 301132301DNAGlycine maxunsure(1)..(301)unsure at all n locations 132tttcttaaat ctaacaatgt tgncttaatc tccaagatgg tttggaccat catggtctct 60attaatctcc acatgacacc taattaagta ttattaattt tttcttgtaa attaacaaaa 120tngagtacat gtcaattaag attattcaag gcaatagaca ggtagtggtt ccaaactact 180tatctatatc ctaagacaac anaaatctan attgagcata taccttgtcc tcgataaggg 240gagagtcatc agcaaaccat gtctcctgtc tctcagactg acaatgagca aaacaagaat 300t 301133301DNAGlycine maxunsure(1)..(301)unsure at all n locations 133aatgatggtt ccttatttat atattttatt ttaatataag attattttta atgaaaatgt 60acaacttaaa ttatttccaa caacatgtta taaggaatta tcttgaaaaa tgaatctaag 120gtttcaatct cattggatag agtctcacaa gtttcttttc aatacactct acatgaaggc 180aactcgtgcg gattggcttg acaagcatgg atcatctcat gatgaagctt tttaatttga 240aatacttatc cacagattat tttagccgat gctntgggga ttatgaggat tcgcgtttag 300t 301134301DNAGlycine maxunsure(1)..(301)unsure at all n locations 134actttgcaat gaganaaaaa cgaaccttgt atctgtcagg gatgaagctc aaaaagtcac 60tctcaagact gtgcaaacac atcaattcga atgagttatg taattaatga tatattgtgg 120atcatacaaa aaaaatgaat acaagtatag ttcactcgtc actgctcatt ttgtaaatct 180tcatgcttgg gcttatacgc cctctagaat atcttcaagg tctggaccac cctgatcttt 240acgttgaagg ttgaaccaat atgccatttt agactttaga gttaagagag agtttacaat 300g 301135301DNAGlycine maxunsure(1)..(301)unsure at all n locations 135tttctgtatt ttctaaatcc aatcccacct ccaacgaaat gtctatagta ttaggcactg 60catattgaga gatatgtatt gaatgttggc naaaaaaaaa tgcactcaaa ctttaaattt 120attaaacgat atagcacang ctttgtcaaa ttctatcagt tttccagcac acagtggtgt 180tatatctgta aattgggtgg atattatgtt ttctatgtag gtggtatttt agacgtggat 240gtatggtttg gagaaatatc aagggaggaa atttactata agtgtgctgg aaaactgata 300g 301136301DNAGlycine max 136cgtgcttctg aaaaagcctt tttggaacac cttttcctag ttatggaaac gctattccaa 60aagcattttt ggttatggga tggtccttcc ggaagttgtt taacatataa ttagatttga 120ttttgttatt ttacagtttt agtaattttt tattttatag atttattctt gatttgtatg 180gaggatgatt acttcaacaa tatgtcagat gaagttggca tggatatgaa tgtgttaact 240gattgacaag tgtaccaaaa gtctaaataa taaagactcg aaagttcgag tgtcgatttc 300c 301137301DNAGlycine max 137taaccattat tgcattgcca tactgttcag cttctcgtag gatatcttct tttaatcgag 60cctccatttt ctctaccctt tcacgaccaa ttccctgaaa gcacaaattg caatatccaa 120ttcagagaat ttggcacaaa attgacaaac tgctgtgaaa ttatgaggca ccaaatggca 180tcttagattc tacttagata agcttcatgc tatatctact tcagcattag catgaatgtg 240tatatttttt gaaatttccc ctaacctgca gcaaacaaag tgaaggggat ttggccagat 300t 301138301DNAGlycine maxunsure(1)..(301)unsure at all n locations 138gccatcgcct ctagcgttgc agttttttgt tttatttttc cctcgccaca cccanaaaag 60gcaaatgagt tgcaatctnt gggaggaaac ctgacccata ncgaattgaa gaaaagctca 120aagtggcaat gtnaagggtc cattnttgtc atcatctctt agtcgaaggc cactatagaa 180gtaggaagga agtgttcacc aatctttgtg ctccccgaca aacctgtaat ttgccgatga 240gttttcgacn acaataattg cactcgaaca cttaccctag ccattttagt ttatcatagc 300t 301139301DNAGlycine maxunsure(1)..(301)unsure at all n locations 139caaactttta tgtgatatcg aaacgactat tgggcttcaa ttaagtagaa acaaagggcc 60tacataggat tattagttta ttaaaacatg tgatgtcacg gtgggccaca ngaagcagta 120acaaaagacc ctcacagtat gggctaatat ttcacgcatt gccgacgtaa cgaggtgtca 180cctttttcac cgtcttcaac atttagccgg gtcactttaa cccgattaca gcccacaaac 240ctgaatagaa acctaatgat ttctctagcc aacacggaac tcatcgttta aatttnnaga 300a 301140301DNAGlycine maxunsure(1)..(301)unsure at all n locations 140atggatgggg tggcaatggc ggaaggaacg gaagagagtg tggcgtcacg gttacgggga 60agggngttga agaaaacgga ggtggttgtg gcggctggtc ttttgaagag ttgaaaagag 120ggggttttgt atatggagat ggaagaagag atacctatgg ttgaagccat ttggagggag 180gaaatgaaag aagaagaaga agaggaggag gcggcgaggg gataaggcca cgcacctttc 240tgtctctgaa cttaaagcct tttaaacctc cctgtgctgc gtgcgtatca ctgtgtgtct 300g 301141301DNAGlycine maxunsure(1)..(301)unsure at all n locations 141taatctctcc caagtccnga ccaaaccaan caatcctctt gataatnggt cttcagtctt 60gatactgtaa ttgtctccac tgattgtgaa tcgtgtgcac atcgtagata cagaaatggt 120tgacttactt caaacacgac gacaggtcct gagtgtatnt ggattttaac taaattaact 180aattgatngg ttattttctc tttcgcacct tgaataccta tcaccgttcg atttcataga 240atgccattgc caccattttt tttttatctt ttttaaattt gctctacnga tttttnctcc 300a 301142301DNAGlycine maxunsure(1)..(301)unsure at all n locations 142ttgattataa gatattatat aaattaccaa aatttctatc attaatttga caaagtacta 60ataacgggtc agaagtgata aggatttata tctcttaatc aagtnntata aggtttgatt 120cctgcacgtt gatntttgaa tatagaataa atcatgttgg accagagaag aatactcatc 180ttgtgtgtca tcattccgac caagattaat caccacttcc aacaaaacta cttcatacta 240atattatcat tagaaaaaaa antaacttaa tgaattttta tgaacaggaa tatagctaca 300t 301143301DNAGlycine max 143ggggcttgac aaactcatca cccccctact tgtcaaggct actaccccga atatcagaca 60atcttcaaca ctggataacc tctttcacct tcaatctagc tcagagcttg gggggcttat 120gtactgtcca gggtccgcaa aatacatgta gcatcctaca tggcactctg aaacttgtgt 180catccctcca tgtcaaccca agaacaggag cactaacatt cgatccttat tagctaggct 240cccccaacca acaggttatc cctaacctct taatatttga atatattgtt accattttat 300c 301144301DNAGlycine maxunsure(1)..(301)unsure at all n locations 144gctgaggtcg aaaaatccga cgattctctg gcgttagagg cttctgctga agatagtgtg 60aaacatgaca gggntgagga agcacaaagt gcaacaccac ccccaacaga aggagatgat 120cacaaggttg agcctgctgt tgcagtagaa aagattgaag attctgtgcc atcagatgaa 180tctgtcgttg cagttgaaga tagtgtaaag gaggagaagg aagtggttcc tgactcacac 240actatcagtg acattgaacc agttcatcag gcnccatcca cagaacaagc tgtggagaaa 300a 301145301DNAGlycine max 145gtttcacttc tttgagtttt ggatttgttg ttattattat aaggaaaggg tttagaataa 60tcatttgaaa ttataagaaa ggatttaatt gattaaagac aagtgcttta atcaattgtc 120atcaggtaga atctaagaaa ttgctaagcc tttagttcgt tttaatacat gtggtcccct 180tatttaaatg tcacttggat atccctcgtg ccctttgttt taagcaaaga taacattatg 240gtacggaggt cattgtcgcc attttccaag gatgcatttt aatttagttt agataacttg 300a 301146301DNAGlycine max 146tccagagctc aacttgttat taggacgtga gataggaaga cttatcagta cgtgagatag 60ataaaagatg aatgcgtgat ttgttactga aggtgattgc aaatatattt gcaaagatat 120gggttgaatg tagataaaaa atgaatcaga ttcagaaata actaaatttt ccaaaataat 180aaaatataaa aagaacgtcg agaatgcaac gtggcttcca acatacatgt tagaacattg 240ttcttgtgac ttcgaaatat cacctcctta aaaatttagt ctcactcaag gaccaacctc 300a 301147301DNAGlycine max 147ctgattagtg tgcgtgtgta gcagctaggt gtcgaatata taaccattgt aatgcattgg 60aaaatcagaa aatgaaggaa ttcagtttaa atatctattt cttctctctt cctaatcttt 120atcgagttct ctcttactgg aaaaaggtct tgtattactt tgttttcgtg aaaagtttaa 180tatcggattt gttggacatg tatacatttt ctatttttct tgtgattttc ataataataa 240aaaaaaaatt ataattttga ttttaatttt caaattcatg attttaaaac atttgatttc 300c 301

Claims

1. A method of creating a population of soybean plants with a low iron growth condition tolerant phenotype, comprising: a. providing a first population of soybean plants; b. detecting in said soybean plant an allele in at least one polymorphic nucleic acid marker locus associated with the low iron growth condition tolerant phenotype wherein the marker locus genetically linked by less than 20 cM to a: i. linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075, ii. linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010, iii. linkage group M genomic region flanked by loci TA75172.sub.--3847 and TC380682, iv. linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870, or v. linkage group O genomic region flanked by loci NA and Cf16144d; c. selecting said plant containing said allele to provide a plant having a genotype associated with a low iron growth condition tolerant phenotype; and d. producing a population of offspring from at least one of said selected soybean plants.

2. A method of claim 1 wherein the marker locus is genetically linked by less than 15 cM to the: a. linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075, b. linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010, c. linkage group M genomic region flanked by loci TA75172.sub.--3847 and TC380682, d. linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870, or e. linkage group 0 genomic region flanked by loci NA and Cf16144d.

3. The method of claim 1 wherein the marker locus is genetically linked by less than 10 cM to the: a. linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075, b. linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010, c. linkage group M genomic region flanked by loci TA75172.sub.--3847 and TC380682, d. linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870, or e. linkage group 0 genomic region flanked by loci NA and Cf16144d.

4. A method of claim 1 wherein the marker locus is in the linkage group J genomic region flanked by loci ASMBL_--10470 and TC370075.

5. The method of claim 1 wherein a second marker locus is in the linkage group E genomic region flanked by loci DB975811 and GLYMA15G06010.

6. The method of claim 1, wherein a second marker locus is in the linkage group M genomic region flanked by loci TA75172.sub.--3847 and TC380682.

7. The method of claim 1, wherein a second marker locus is in the linkage group D2 genomic region flanked by loci TC350035 and Gm_W82_CR17.G8870.

8. The method of claim 1 wherein a second marker locus is in the linkage group 0 genomic region flanked by loci NA and Cf16144d.

9. A method of creating a population of soybean plants comprising at least one allele associated with the low iron growth condition tolerant phenotype comprising at least one of SEQ ID NOS 1-147, the method comprising the steps of: a. genotyping a first population of soybean plants, said population containing at least one allele associated with the low iron growth condition tolerant phenotype, the at least one allele associated with the low iron growth condition tolerant phenotype comprising at least one of SEQ ID NOS 1-147; b. selecting from said first population one or more identified soybean plants containing said at least one allele associated with the low iron growth condition tolerant phenotype comprising at least one of SEQ ID NOS 1-147; and c. producing from said selected soybean plants a second population, thereby creating a population of soybean plants comprising at least one allele associated with the low iron growth condition tolerant phenotype comprising at least one of SEQ ID NOS 1-147.