Novel Restriction Endonucleases, DNA Encoding These Endonucleases and Methods for Identifying New Endonucleases with the Same or Varied Specificity

Abstract

Specified restriction endonucleases have been characterized for the first time by their amino acid and DNA sequences. These sequences and those with at least 90% identity thereto have been used as probes in sequence similarity analyses to identify sequence matches in a sequence database that corresponds to novel restriction endonucleases or isoschizomers. The sequence similarity analyses includes selecting a positive sequence match from any sequence producing an expectation value of less than or equal to e-02.

Description

CROSS REFERENCE

[0001] This application is a continuation of U.S. application Ser. No. 13/490,100, filed on Jun. 6, 2012 which is a divisional of U.S. application Ser. No. 11/997,614, filed on Feb. 1, 2008 which is a §371 application of international application number PCT/US2006/030419 filed on Aug. 3, 2006, which claims priority from U.S. provisional application No. 60/705,504 filed on Aug. 4, 2005, herein incorporated by reference.

BACKGROUND

[0002] Restriction endonucleases are enzymes that occur naturally in certain unicellular microbes--mainly bacteria and archaea--and that function to protect those organisms from infections by viruses and other parasitic DNA elements. These enzymes bind to specific sequences of nucleotides (recognition sequence') in double-stranded DNA molecules (dsDNA) and cleave the DNA, usually within or close to the recognition sequence, disrupting the DNA and triggering its destruction. Restriction endonucleases commonly occur with one or more companion enzymes termed modification DNA methyltransferases. DNA methyltransferases bind to the same sequences in dsDNA as the restriction endonucleases they accompany, but instead of cleaving the DNA, they alter it by the addition of a methyl group to one of the bases within the sequence. This modification (methylation') prevents the restriction endonuclease from binding to that site thereafter, rendering the site resistant to cleavage. Methyltransferases function as cellular antidotes to the restriction endonucleases they accompany, protecting the cell's own DNA from destruction by its restriction endonucleases. Together, a restriction endonuclease and its companion modification methyltransferase(s) form a restriction-modification (R-M) system, an enzymatic partnership that accomplishes for microbes what the immune system accomplishes, in some respects, for multicellular organisms.

[0003] A large and varied class of restriction endonucleases has been classified as `Type II` class of restriction endonucleases. These enzymes cleave DNA at defined positions, and when purified can be used to cut DNA molecules into precise fragments for gene cloning and analysis.

[0004] New Type II restriction endonucleases can be discovered by a number of methods. The traditional approach to screening for restriction endonucleases, pioneered by Roberts et al. and others in the early to mid 1970's (e.g. Smith, H. O. and Wilcox, K. W., J. Mol. Biol. 51:379-391 (1970); Kelly, T. J. Jr. and Smith, H. O., J. Mol. Biol. 51:393-409, (1970); Middleton, J. H. et al., J. Virol. 10:42-50 (1972); and Roberts, R. J. et al., J. Mol. Biol. 91:121-123, (1975)), was to grow small cultures of individual strains, prepare cell extracts and then test the crude cell extracts for their ability to produce specific fragments on small DNA molecules (see Schildkraut, I. S., "Screening for and Characterizing Restriction Endonucleases", in Genetic Engineering, Principles and Methods, Vol. 6, pp. 117-140, Plenum Press, NY, N.Y. (1984)). Using this approach, about 12,000 strains have been screened worldwide to yield the current harvest of almost 3,600 restriction endonucleases (Roberts, R. J. et al., Nucl. Acids. Res. 33:D230-D232 (2005)). Roughly, one in four of all strains examined, using a biochemical approach, show the presence of a Type II restriction enzyme.

[0005] An in silico screening technique to identify restriction-modification systems has also been described and has been successfully used to identify novel restriction endonucleases (US-2004-0137576-A1). This method relies on identifying new methylases by their consensus sequences. Methylases have much more conservation of amino acid sequence, because they all must bind the methyl donor cofactor S-adenosyl methionine (SAM) and bind the nucleotide to be methylated, either an adenine or a cytosine base, and then perform the methyl transfer chemistry. Although there are several classes of methyltransferases, there are many sequenced examples of methylases and these have well conserved motifs that can be used to identify a protein sequence in a database as a methylase. In this method, identifying restriction endonucleases relies on testing any or all open reading frame (ORF) protein sequences located near the identified methylases.

[0006] Since the various Type II restriction enzymes appear to perform similar biological roles and share the biochemistry of causing dsDNA breaks, it might be thought that they would closely resemble one another in amino acid sequence. Experience shows this not to be true, however. Surprisingly, far from sharing significant amino acid similarity with one another, most enzymes appear unique, with their amino acid sequences resembling neither other restriction enzymes nor any other known proteins. Thus the Type II restriction endonucleases seem either to have arisen independently of each other during evolution or to be evolving very rapidly thereby losing apparent sequence similarity, so that today's enzymes represent a heterogeneous collection rather than one or a few distinct families.

[0007] Restriction endonucleases are biochemically diverse in their function: some act as homodimers, some as monomers, others as heterodimers. Some bind symmetric sequences, others asymmetric sequences; some bind continuous sequences, others discontinuous sequences; some bind unique sequences, others multiple sequences. Some are accompanied by a single methyltransferase, others by two, and yet others by none at all. When two methyltransferases are present, sometimes they are separate proteins; at other times they are fused. The orders and orientations of restriction and modification genes vary, with all possible organizations occurring. Given this great diversity among restriction endonucleases, it is perhaps not surprising that it has not been possible to form consensus sequences that can be used for in silico searches that are able to identify Type II restriction endonucleases, as has been successfully done for DNA methyltransferases. Thus there is no general common amino acid sequence motif(s) that can be used to identify restriction endonucleases from translated raw DNA sequence ab initio.

[0008] Although restriction endonucleases lack conserved sequence motifs and generally have highly diverged DNA and amino acid sequences, some restriction endonucleases are in fact related to one another and, though they may diverge in function, these endonucleases or families of related endonucleases share significant sequence similarity with one another. The key to unlocking these families of endonucleases is to obtain the sequence of one of the members of the related enzymes; from this sequence the other members of the family can be identified. With the advent of whole genome sequencing, many prokaryotic DNA sequences, and from the DNA sequence many amino acid sequences, have become available. Thus there are many amino acid sequences in the database with no known function. This pool of sequences undoubtedly contains numerous restriction endonucleases. The problem is how to identify which genes encode restriction endonucleases, and then how to characterize the function of these genes.

SUMMARY

[0009] In an embodiment of the invention, a DNA segment encoding a restriction endonuclease and the corresponding amino acid sequence is described where the DNA has at least 90% sequence identity with a DNA sequence selected from SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, and 187 or the amino acid sequence has at least 90% sequence identity with an amino acid sequence selected from SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186 and 188.

[0010] In an additional embodiment of the invention, an identified restriction endonuclease is provided which has an amino acid sequence identified by an Expectation value of less than or equal to e-02 in a BLAST search when an amino acid sequence having at least 90% sequence identity to the amino acid sequences listed above is used for searching the database. In the context of the embodiments of the invention, "an" is not intended to be limited to "one."

[0011] In an additional embodiment of the invention, a method is provided for identifying a restriction endonuclease, that includes: (a) selecting one or more probes having at least 90% sequence identity to a sequence selected from SEQ ID NOS:1-153 and 156-188; (b) comparing the one or more probes with a database of sequences by a sequence similarity analysis to identify a sequence match; and (c) identifying the restriction endonuclease from the sequence match.

[0012] Examples of a sequence similarity analysis include a BLAST search and a PSI Blast search using an expectation value, E of less than or equal to e-02 (E<e-02).

[0013] The sequence similarity analysis may further include analyzing the expectation value assigned to a sequence match and the distribution of sequence similarity between the probe and the sequence match.

[0014] In another embodiment, the sequence similarity analysis utilizes sequence comparisons such that, where the amino acid sequence match is in a genome sequence within the database, a methyltransferase sequence adjacent to the restriction endonuclease sequence can be identified and characterized by sequence similarity analysis to provide information about the restriction endonuclease.

[0015] In another embodiment of the invention, the restriction endonuclease is further characterized by biochemistry to determine the recognition and cleavage sites for the restriction endonuclease. The functional characteristics of the restriction endonuclease may further be characterized by thermostability, pH range and optima, star activity, etc. using methodology described in the prior art.

[0016] In a further embodiment of the invention, a method for identifying a restriction endonuclease is provided that includes: (a) selecting one or more probes having at least 90% sequence identity to a known restriction endonuclease sequence; (b) comparing the one or more probes with a database of sequences by a sequence similarity analysis to identify a sequence match, wherein a sequence match is any sequence producing an expectation value, E≦e-02; and (c) identifying the restriction endonuclease from the sequence match.

DETAILED DESCRIPTION OF THE DRAWINGS

[0017] FIG. 1 (1-1 to 1-6) shows a list of restriction enzymes and their respective recognition sequences.

[0018] FIG. 2 (2-1 to 2-58) shows the DNA and amino acid sequences of the restriction enzymes listed in FIG. 1.

[0019] FIG. 3 (3-1 to 3-2) shows the number of observed matches at Expectation values ranging from E=e-01 to E=e-10 using targeting sequences selected from FIG. 2 in a sequence similarity analysis using the GENBANK non-redundant database and a BLAST similarity search.

[0020] For each enzyme, the following steps were performed:

[0021] 1. GenBank's NR database (July, 2006 release) was queried with the enzyme's amino acid sequence using the BLAST program [1] with its low complexity filter turned off.

[0022] 2. The first 64 hits were taken from BLAST's output and counted the number of hits with e-values lower than 10^-x (x=10, 9, . . . , 1).

REFERENCES

[0022]

[0023] 1. BLASTP 2.2.10 [Oct. 19, 2004]

[0024] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.

[0025] FIGS. 4A-4D provide a list of restriction endonucleases identified by PSI BLAST searches using BclI and EagI amino acid sequences.

[0026] FIG. 4A shows sequences with significant alignments to BclI.

[0027] FIG. 4B shows PSI-BLAST alignments between BclI, "query" sequence, and the restriction endonucleases identified, the "Sbjct" sequences. Identities and positives (similar amino acids) are shown between the query and subject sequences.

[0028] FIG. 4C shows PSI-BLAST significant alignments to EagI.

[0029] FIG. 4D shows PSI-BLAST alignments between EagI, "query" sequence, and the restriction endonucleases identified, the "Sbjct" sequences. Identities and positives (similar amino acids) are shown between the query and subject sequences.

DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The amino acid sequences for a group of restriction enzymes are described herein for the first time. The use of these sequences and corresponding DNA sequences are described in a novel method for finding restriction endonucleases in a sequence database. Statistically significant sequence similarity between known restriction endonucleases and sequences in the database can reveal new and useful tools for molecular biology in the form of isochizomers, neoschizomers and restriction endonucleases with novel recognition sequences.

[0031] Embodiments of the method are based on finding at least a minimum amount of sequence similarity between an amino acid or DNA sequence in a sequence database and a probe. (A "probe" may refer to an amino acid sequence or significant portion thereof (or a DNA sequence or significant portion thereof) of the specified restriction endonuclease). The identified sequence having similarity to a probe may be referred to as "a sequence match". In one embodiment, the sequence match is assumed to be a restriction endonuclease if an expectation value requirement of E=e<-02 is met. Analysis of sequence similarity between the probe and the sequence match indicates whether the newly identified restriction endonuclease is likely to be an isochizomer of a known restriction endonuclease, or a new restriction endonuclease recognizing and/or cleaving at a previously unknown site(s). In addition, the determination of the amino acid or DNA sequence similarity of any methyltransferase encoded by a DNA sequence that is located at an adjacent site on a genome to a gene encoding the newly identified restriction endonuclease can assist in classifying the restriction endonuclease. Confirmation of the recognition and cleavage sites of a restriction endonuclease identified by sequence similarity analysis can be achieved by biochemistry methods described herein as well as the biochemical properties of the enzyme such as pH and heat sensitivity.

[0032] In silico screening has established that similarity occurs between restriction enzyme gene sequences only when the two enzymes are related to one another. Such related restriction endonucleases may be isoschizomers; i.e. they recognize exactly the same sequence and cut at the same position, but come from different microorganisms (e.g. Lubys, A. et al., Gene 141:85-89 (1994); Withers, B. E. et al., Nucl. Acids. Res. 20:6267-6273 (1992)); have related recognition sequences (i.e., they recognize sequences that may be subsets of each other (e.g. HincII GTYRAC and MjaIV GTNNAC (US-2004-0137576-A1)) or share several common nucleotides (e.g., BsaI GGTCTC and BsmBI CGTCTC), or have similar catalytic properties but recognize different DNA sequences, such as MmeI [TCCRACN20/N18 (SEQ ID NO:154)] and CstMI [AAGGAGN20/N18 (SEQ ID NO:155)].

[0033] Although restriction endonucleases cannot be identified from common sequence motifs, a method is provided herein to search a sequence database for significant matches (sequence matches) to sequences of specified restriction endonucleases (probes). It is anticipated that sequence matches and probes may be derived from a common ancestor. Some of the sequence matches may be isoschizomers. However probes can be used to successfully identify novel restriction endonucleases that recognize and/or cleave DNA sequences that are different from that recognized and/or cleaved by the probes, and which may be novel DNA recognition sequences. In principle, any amino acid sequence of a restriction endonuclease having at least 90% identity with a sequence of a specified restriction endonuclease can be tested in databases to discover previously unknown restriction endonucleases. A limitation on discovery of novel restriction endonucleases in sequence databases is thus the availability of probes.

[0034] The likelihood that a sequence identified by this method will in fact be a restriction endonuclease depends on the choice of the level of statistical significance used for the identification of restriction endonuclease candidates; i.e. a less stringent threshold of statistical significance will result in more false positive hits (sequences that are not restriction endonucleases), but will include those restriction endonucleases (true positives) that are more highly diverged from the known restriction endonuclease and thus perhaps more likely to have a different recognition sequence or different biochemical characteristics. Conversely, a more stringent threshold will insure that most or all of the hits are restriction endonuclease genes (few false positives), but will likely exclude some restriction endonucleases (false negatives) having relatively less sequence similarity. Thus, selection of the probability functions determines the stringency of the selection and the likelihood that candidate sequences identified are in fact restriction endonucleases. We use the Expectation value of the BLAST program to set the cutoff for finding restriction endonucleases. When the Expectation value is E=e-02 or less, the sequence match can be predictably identified as a restriction endonuclease. Close proximity to a methylase is further confirmation that the sequence is an endonuclease, and the methylase can be used to protect host cells when expressing the potential endonuclease in a new host.

[0035] The specified amino acid and nucleic acid sequences for the restriction endonucleases provided in FIG. 2 are previously unknown and result from the cloning and sequencing of these restriction endonucleases, along with, in most cases, their methyltransferase(s) using biochemical methods. Biochemical methods for obtaining purified restriction enzymes and their sequences include the methylase selection method (see U.S. Pat. No. 5,179,015, U.S. Pat. No. 5,137,823, U.S. Pat. No. 5,180,673, U.S. Pat. No. 5,200,333, U.S. Pat. No. 5,320,957), while specific examples of cloning and/or purification for individual restriction endonucleases are U.S. Pat. No. 5,405,768, U.S. Pat. No. 5,004,691, U.S. Pat. No. 5,030,569, U.S. Pat. No. 5,366,882, U.S. Pat. No. 5,137,823, U.S. Pat. No. 5,198,354, U.S. Pat. No. 5,434,068, U.S. Pat. No. 5,354,680, U.S. Pat. No. 4,996,151, U.S. Pat. No. 4,999,294, U.S. Pat. No. 5,196,332, U.S. Pat. No. 4,987,074, U.S. Pat. No. 4,999,293, US RE:35,248, U.S. Pat. No. 5,215,906, U.S. Pat. No. 4,983,522, U.S. Pat. No. 5,298,404, U.S. Pat. No. 5,147,794, U.S. Pat. No. 5,196,331, U.S. Pat. No. 5,053,330, U.S. Pat. No. 5,292,651, U.S. Pat. No. 5,202,248, U.S. Pat. No. 5,139,942, U.S. Pat. No. 5,278,060, U.S. Pat. No. 5,075,232, U.S. Pat. No. 5,371,006, U.S. Pat. No. 5,288,696, U.S. Pat. No. 5,637,476, U.S. Pat. No. 5,262,318, U.S. Pat. No. 5,296,371, U.S. Pat. No. 5,208,157, U.S. Pat. No. 4,983,542, U.S. Pat. No. 5,002,882, U.S. Pat. No. 5,516,678, U.S. Pat. No. 5,543,308, U.S. Pat. No. 5,532,153, U.S. Pat. No. 5,731,185, U.S. Pat. No. 5,616,484, U.S. Pat. No. 5,721,126, U.S. Pat. No. 5,824,529, U.S. Pat. No. 5,663,067, U.S. Pat. No. 5,786,195, U.S. Pat. No. 5,945,326, U.S. Pat. No. 5,849,558, U.S. Pat. No. 5,866,422, U.S. Pat. No. 5,866,398, U.S. Pat. No. 5,945,288, U.S. Pat. No. 5,885,818, U.S. Pat. No. 6,004,793, U.S. Pat. No. 6,027,929, U.S. Pat. No. 6,025,179, U.S. Pat. No. 6,048,731, U.S. Pat. No. 6,258,583, U.S. Pat. No. 6,238,901, U.S. Pat. No. 6,130,078, U.S. Pat. No. 6,048,719, U.S. Pat. No. 6,133,008, U.S. Pat. No. 6,245,545, U.S. Pat. No. 6,066,487, U.S. Pat. No. 6,194,188, U.S. Pat. No. 6,133,009, U.S. Pat. No. 6,335,190, U.S. Pat. No. 6,403,354, U.S. Pat. No. 6,395,531, U.S. Pat. No. 6,391,608, U.S. Pat. No. 6,514,737, U.S. Pat. No. 6,764,843, U.S. Pat. No. 6,596,524, U.S. Pat. No. 6,593,122, U.S. Pat. No. 6,589,769, U.S. Pat. No. 6,586,220, U.S. Pat. No. 6,673,588, U.S. Pat. No. 6,723,546, U.S. Pat. No. 6,794,172, U.S. Pat. No. 6,869,786. Methods of sequencing are well known in the art.

In Silico Searching for Restriction Endonucleases

[0036] There is no characteristic consensus amino acid sequence for type II restriction enzymes that can be used to identify a new example from a database, even though all restriction endonucleases recognize and cleave dsDNA.

[0037] There is an "endonuclease motif" that many of the restriction endonucleases share, consisting of ((DM X_6-20 (D/E)XK). However, this motif does not contain enough specified sequence to be used for database searching (Anderson, J. E.; Curr. Opin. Struct. Biol. 3: 24-30 (1993)).

[0038] As illustrated in the Examples, candidate sequences that produce Expectation values of equal to or less than e-02 when compared to any of the restriction endonuclease sequences specified in this application are likely to be restriction endonucleases. Restriction endonucleases identified from sequence matches are likely to recognize the same or related DNA sequences as the specified endonuclease with which they share significant similarity. Nonetheless, enzymes thus identified may differ in useful ways from the known restriction endonuclease with which they share sequence similarity, including having different recognition sequences, different biochemical properties, such as reaction conditions, temperature optima, methylation sensitivity, etc., or other different characteristics from the previously known endonuclease. One example of isoschizomers, which have different properties, is an isochizomer of thermolabile SapI identified as BspQI, which is the thermostable.

[0039] Accordingly, sequence matches may correspond to restriction endonucleases that are isoschizomers, which recognize an identical sequence and cleave at the same position; neoschizomers, which recognize the same DNA sequence but cleave at a different position within the DNA; or restriction endonucleases that have similar but different recognition sequences. An example of the latter is BsaI, which produces a BLAST E-value of e^-66 (NR Genbank database) with BsmBI, yet differs subtly in the recognition sequence as follows:

[0040] BsaI: GGTCTC

[0041] BsmBI: CGTCTC

[0042] Although certain restriction enzymes listed in FIG. 1 have been identified from a variety of organisms and their recognition sequences determined, the DNA and amino acid sequences of these enzymes have not previously been obtained. These sequences are here provided in FIG. 2 and may be used not only for cloning the identified naturally occurring restriction enzymes, but also for discovering related enzymes using sequence databases such as GenBank (Benson et al., Nucleic Acids Research 32:D23-D26 (2004)) using a suitable search algorithm. Sequence similarity may be determined using in silico sequence matching programs such as BLAST ((web site: http://www.ncbi.nlm.nih.gov/BLAST/), Altschul, S.F., et al., J. Mol. Biol. 215:403-410 (1990) or Smith, T. F. and Waterman, M. S., J. Mol. Biol. 147(1): 195-7 (1981), or PSI-BLAST ((web site: http://www.ncbi.nlm.nih.gov/BLAST/), Altschul et al. Nucleic Acids Res. 25:3389-3402 (1997)) available in the art, or by in vitro biochemical assays such as Southern hybridization assays. An example of a search methodology is provided in Example I.

[0043] Significant sequence similarity between any one of the amino acid sequences or DNA sequences of the restriction endonuclease listed in FIG. 2 or more generally in REBASE (http://rebase.neb.com) and an unknown sequence in the database suggests that the unknown sequence is a restriction endonuclease also. Sequence similarity can be determined from alignment algorithms described above, where an Expectation value of less than e-02 is considered significant, and thus the sequence is a restriction endonuclease.

[0044] Any single alignment producing an Expectation value of less than e-02 between an amino acid sequence of FIG. 2 and an unknown sequence in the database, particularly where the unknown sequence does not appear to have sequence similarity with other sequences in the database, suggests a strong presumption that the unknown sequence is a restriction endonuclease (FIG. 3). When Expectation values are greater than e-02 but less than 2 between two sequences, the unknown sequence identified by the method may be presumed to be an endonuclease if there is additional supporting information, such as that the unknown sequence is located next to a methylase in its genome sequence context. The presence of a methylase gene adjacent or close to the unknown sequence identified by the method strengthens the likelihood that the unknown sequence is indeed an endonuclease.

[0045] DNA and amino acid sequences of specified restriction endonucleases and methylases provided in FIG. 2 are as follows.

[0046] AciI (SEQ ID NOS:1 and 2), AclI (SEQ ID NOS:3 and 4), AflII (SEQ ID NOS:5 and 6), AflIII (SEQ ID NOS:7 and 8), ApaI (SEQ ID NOS:9 and 10), ApoI (SEQ ID NOS:11 and 12), AscI (SEQ ID NOS:13 and 14), AseI (SEQ ID NOS:15 and 16), AspCNI (SEQ ID NOS:17 and 18), AvrII (SEQ ID NOS:19 and 20), BbvI (SEQ ID NOS:21 and 22), BbvCIA (SEQ ID NOS:23 and 24), BbvCIB (SEQ ID NOS:145 and 146), BccI (SEQ ID NOS:25 and 26), BceAI (SEQ ID NOS:27 and 28), BclI (SEQ ID NOS:29 and 30), BfaIA (SEQ ID NOS:31 and 32), BfaIB (SEQ ID NOS:147 and 148), BfuAI (SEQ ID NOS:33 and 34), BlpI (SEQ ID NOS:35 and 36), BmrI (SEQ ID NOS:37 and 38), BsaJI (SEQ ID NOS:39 and 40), BscGI (SEQ ID NOS:41 and 42), BseYIA (SEQ ID NOS:43 and 44), BseYIB (SEQ ID NOS:87 and 88), BsgI (SEQ ID NOS:45 and 46), BspCNI (SEQ ID NOS:47 and 48), BspHI (SEQ ID NOS:49 and 50), BspMI (SEQ ID NOS:51 and 52), BsrBI (SEQ ID NOS:53 and 54), R1.BsrDI (SEQ ID NOS:55 and 56), R2.BsrDI (SEQ ID NOS:57 and 58), BsrI (SEQ ID NOS:59 and 60), BstEII (SEQ ID NOS:61 and 62), BsuFI (SEQ ID NOS:63 and 64), Bsu36I (SEQ ID NOS:65 and 66), Cac8I (SEQ ID NOS:67 and 68), ClaI (SEQ ID NOS:69 and 70), CviKI (SEQ ID NOS:71 and 72), Dral (SEQ ID NOS:73 and 74), EagI (SEQ ID NOS:75 and 76), Earl (SEQ ID NOS:77 and 78), EsaBC3I (SEQ ID NOS:79 and 80), EsaBC4I (SEQ ID NOS:81 and 82), EsaBS9I (SEQ ID NOS:83 and 84), EsaDix61P (SEQ ID NOS:85 and 86), EsaLHCI (SEQ ID NOS:89 and 90), EsaS11P (SEQ ID NOS:91 and 92), FseI (SEQ ID NOS:93 and 94), FspI (SEQ ID NOS:95 and 96), HhaI (SEQ ID NOS:97 and 98), HinP1I (SEQ ID NOS:99 and 100), MfeI (SEQ ID NOS:101 and 102), MluI (SEQ ID NOS:103 and 104), MmeII (SEQ ID NOS:105 and 106), MscI (SEQ ID NOS:107 and 108), NdeI (SEQ ID NOS:109 and 110), NgoMX (SEQ ID NOS:111 and 112), NotI (SEQ ID NOS:113 and 114), PacI (SEQ ID NOS:115 and 116), PflMI (SEQ ID NOS:117 and 118), PmeI (SEQ ID NOS:119 and 120), PshAI (SEQ ID NOS:121 and 122), PstII (SEQ ID NOS:123 and 124), PsuNI (SEQ ID NOS:125 and 126), SacII (SEQ ID NOS:127 and 128), SfoI (SEQ ID NOS:129 and 130), SpeI (SEQ ID NOS:131 and 132), T111 (SEQ ID NOS:133 and 134), TseI (SEQ ID NOS:135 and 136), Tsp509I (SEQ ID NOS:137 and 138), Tth111I (SEQ ID NOS:139 and 140), XcmI (SEQ ID NOS:141 and 142), XhoII (SEQ ID NOS:143 and 144), Acc65I (SEQ ID NOS:149 and 150), BsaAI (SEQ ID NOS:151 and 152), BsmFI (SEQ ID NOS:153 and 156), BspEI (SEQ ID NOS:157 and 158), BtsCI (SEQ ID NOS:159 and 160), BtsIA (SEQ ID NOS:161 and 162), BtsIB (SEQ ID NOS:163 and 164), EcoNI (SEQ ID NOS:165 and 166), Fnu4HI (SEQ ID NOS:167 and 168), KasI (SEQ ID NOS:169 and 170), McaTI (SEQ ID NOS:171 and 172), NciI (SEQ ID NOS:173 and 174), NruI (SEQ ID NOS:175 and 176), Sbo13I (SEQ ID NOS:177 and 178), SfcI (SEQ ID NOS:179 and 180), Stul (SEQ ID NOS:181 and 182), Styl (SEQ ID NOS:183 and 184), BsiWI (SEQ ID NOS:185 and 186), and BspQI (SEQ ID NOS:187 and 188).

[0047] All references described herein, as well as U.S. provisional application Ser. No. 60/705,504, are incorporated by reference. The examples are not intended to be limiting.

EXAMPLES

Example 1

Method of Identifying Potential Restriction Endonuclease Using the Seauences Provided in this Application

[0048] The amino acid sequence of one of the known restriction endonucleases is used to perform a protein to protein (blastp) BLAST search, a protein to translated database (tblastn) BLAST search, or is used to perform a protein to protein PSI-BLAST iterative similarity search. For example, such a search may be performed through the NCBI web server: http://www.ncbi.nlm.nih.gov/blast/ selecting the blastp (or tblastn) program, and searching against the NR (non-redundant) database of "all organisms," using the standard preset values, which consist of Expect=10, word size=3, using the BLOSUM62 matrix and with gap costs of Existence=11, extension=1. The low complexity filter can be turned off for the search, as restriction endonucleases are prokaryotic proteins and generally do not contain repeats or regions of low complexity. These parameters can be varied by those skilled in the art to obtain slightly varied search results. The PSI-BLAST search can be performed for several iterations until convergence is achieved.

[0049] The output returned by the BLAST search is examined for sequences that give Expectation scores of less than or equal to e-02. These sequences are presumed to be restriction endonucleases.

[0050] The sequence context of the restriction endonucleases identified is examined to see if there is a DNA methyltransferase adjacent to or near (within one or two ORFS) the restriction endonuclease. The presence of such a methyltransferase is highly suggestive that the sequence identified using the known endonuclease sequence is an endonuclease.

[0051] The degree of similarity between the known endonuclease sequence (probe) and the sequence match can suggest whether the two sequences are isoschizomers (indicated by a high degree of similarity, for example E<e-50), or may recognize related but different sequences (indicated by a lesser degree of similarity, for example, e-10<E<e-02).

[0052] The sequence match is tested to see if it encodes a functional restriction endonuclease by any convenient methods of expressing protein from the sequence and testing that protein for endonucleolytic function, such as the methods described in Example 3. For example, the identified sequence may by amplified by PCR. The gene may then be expressed either in a cell-free in vitro transcription/translation system and the protein produced tested for endonuclease activity, or the gene may be introduced into a vector and cloned into a host cell, such as E. coli. The transformed host cells are then grown to allow the identified endonuclease gene to express protein, and a cell free lysate is prepared and tested for endonuclease activity.

Example 2

Search Results for Several of the Enzymes of this Application

[0053] A. The alignments produced by standard BLAST or PSI-BLAST is here used to predict whether a sequence match encodes or represents a newly identified restriction endonuclease and whether this restriction endonuclease is likely to have a similar substrate specificity or whether it is likely to recognize a different DNA sequence to that of the restriction endonuclease corresponding to the probe (specified restriction endonuclease). The level of sequence similarity between the probe and the sequence match and the distribution of the similarity can be used to predict whether two restriction endonucleases recognize the same or different sites on a DNA substrate. A sequence match with significant similarity to the probe spread throughout the sequences is likely to be an isoschizomer that recognizes the same DNA substrate as the specified restriction endonuclease, with the likelihood increasing as the level of similarity increases. However, a newly identified restriction endonuclease that shares significant similarity with the specified restriction endonuclease in only a portion of its sequence is less likely to be isoschizomer. In addition, a comparison of a methyltransferase encoded by a gene adjacent to the sequence match, if there is one, with methyltransferases of known DNA recognition specificity, can assist in this analysis. If the adjacent methyltransferase is most similar to methyltransferase(s) that recognize the same sequence as the specified restriction endonuclease, the likelihood that the two restriction endonucleases are isoschizomers is increased; however if the methyltransferase is most similar to methyltransferase(s) recognizing different sequences from the specified restriction endonuclease, then the likelihood that the identified restriction endonuclease differs from the recognition sequence of the specified restriction endonuclease is increased.

[0054] Results for searching using the BclI amino acid sequence on 25 Jul. 2006 (scores will change slightly as the size of the database increases, and new hits may be found as new sequences are added to the database daily). The BclI amino acid sequence from FIG. 2 was copied and pasted into the NCBI Blast web server at: http://www.ncbi/nlm.nih.gov/BLAST/ using the "Protein-protein BLAST (blastp) program. The default values for the search were used and the low complexity filter was turned off. The best scoring results are reported below:

TABLE-US-00001 Score E Sequences producing significant alignments: (Bits) Value gi|27657790|gb|AAO18218.1|Yga2E [Corynebacterium glutamicum] . . . 159 1e-37 gi|68248271|gb|EAN30354.1|hypothetical protein Mmc1DRAFT_368 . . . 103 7e-21 gi|110287792|sp|Q29L39|POE_DROPS Protein purity of essence >g . . . 36.2 1.6 gi|90302769|gb|EAS32400.1|hypothetical protein CIMG_03424 [Cocc 35.0 3.8 gi|89286118|gb|EAR84121.1|hypothetical protein TTHERM_007230 . . . 34.7 5.4 gi|4426611|gb|AAD20450.1|pushover [Drosophila melanogaster] 34.3 6.9

[0055] The first two hits have E values of 1e-37 and 7e-21, both of which would indicate that these are highly likely to be restriction endonucleases. Note that the both sequences identified using BclI are reported as "hypothetical proteins," since without a sequence of a known endonuclease with which to identify these sequences, such as the BclI sequence specified in this application, the nature of these ORFs was not known. Both of these ORFs are adjacent to an ORF identified as a methyltransferase, which strengthens the case that they are endonucleases. These ORFs can be confirmed as endonucleases, and their characteristics determined, by the techniques described in Example 3. The third and subsequent hits are outside the threshold value of E value <e-02. Note the very sharp drop off in E value results between the two endonuclease hits and the next best scoring sequences, which are outside the level of significance for identifying endonucleases described herein.

[0056] Similar results are obtained using the PSI-BLAST program with the Bcll sequence. PSI-BLAST is able to detect more distant similarities among protein sequences than standard BLAST. The expectation values produced after several iterations of PSI-BLAST are often smaller than the single pass results from standard BLAST. PSI-BLAST may detect restriction endonuclease candidate genes whose sequence is more distantly similar to the input restriction endonuclease sequence; however, PSI-BLAST errors may be amplified by the iteration process and so marginal hits with PSI-BLAST require more careful examination. A PSI-BLAST search with the Bcll amino acid sequence yields the same two hits as the standard BLAST search, although the E values are now much smaller. The Bcll amino acid sequence from FIG. 2 was copied and pasted into the NCBI Blast web server at: http://www.ncbi/nlm.nih.gov/BLAST/ using the PSI-BLAST program. The default values for the search were used. The best scoring results are reported below:

TABLE-US-00002 Score E (Bits) Value Sequences producing significant alignments: gi|27657790|gb|AAO18218.1|Yga2E [Corynebacterium glutamicum] . . . 431 2e-119 gi|68248271|gb|EAN30354.1|hypothetical protein Mmc1DRAFT_368 . . . 242 1e-62 Sequences with E-value WORSE than threshold gi|89286118|gb|EAR84121.1|hypothetical protein TTHERM_007230 . . . 37.0 0.93 gi|38570107|ref|NP_073622.2|CAP-binding protein complex inte . . . 36.3 1.6 gi|8670820|emb|CAA71749.1|CAP-binding protein complex intera . . . 36.3 1.7

[0057] Using the Bcll sequence of FIG. 2 in the method described clearly identifies two restriction endonuclease genes.

[0058] The PSI-BLAST output returns the alignment that produced the E values given. This alignment may be used to predict whether the restriction endonuclease identified will be an isoschizomer of BclI or will recognize a differing sequence. Here the best scoring sequence, gi:27657790 Yga2E hypothetical protein has highly significant similarity (E=2e-119) that extends over nearly the entire sequence of BclI (from amino acids 1 to 277 out of a total length of 285 amino acids) and over nearly the entire length of Yga2E (amino acids 1 to 292 out of a total length of 306 amino acids). The adjacent methyltransferase sequence best matches the M.BclI methyltransferase sequence, at an E value of e-72, having similarity throughout the sequence, and in particular in the TRD (Target (DNA) Recognition Domain) of the methyltransferase. This evidence combines to make it very likely that this restriction endonuclease gene will recognize the same sequence, TGATCA, as BclI, though it may differ in other characteristics, such as temperature optima and sensitivity, buffer optima, etc. However, the second hit, gi:68248271 hypothetical protein Mmc1DRAFT_--3688, appears likely to recognize a different sequence than BclI and is thus a good candidate for a new DNA specificity. This restriction endonuclease sequence has significant similarity to BclI (E=1e-62), but in contrast to the first hit, this similarity is only to the carboxy half of BclI. The approximately first half of each restriction endonuclease does not share significant similarity. In addition, the adjacent methyltransferase has significant similarity in a standard BLAST search to methyltransferases recognizing many different, though related, DNA sequences, and the similarity is at similar levels of E value and distribution throughout the methyltransferase sequence. For example, a BLAST search with this methyltransferase, Mmc1DRAFT_--3689, gave E value scores of e-57 to M.EacI which recognizes GGATC, e-54 to M.ThaII, recognizing GATC, e-26 to M1.BstSE1, recognizing GAGTC, e-26 to M.BstNBI, recognizing GASTC, e-20 to M.CviBI, recognizing GANTC, e-19 to M.EcoRV, recognizing GATATC and e-14 to M.TfiI, recognizing GAWTC, among many other methyltransferases. The E value for this adjacent methyltransferase and the BclI methyltransferase, M.BclI, was E=e-12. This significant but relatively equal similarity to enzymes with a wide range of recognition sequences, coupled with the finding that the significant similarity between the identified and input RE sequenced was limited to only one half of the restriction endonuclease genes indicates that the identified Mmc1DRAFT_--3688 is likely to recognize a different sequence than the input restriction endonuclease, BclI.

[0059] B. Results for searching using the Eagl amino acid sequence of FIG. 2 on 25 Jul. 2006 as in A above. The best scoring results from a BLAST search are reported below:

TABLE-US-00003 Score E Sequences producing significant alignments: (Bits) Value gi|84686807|ref|ZP_01014694.1|hypothetical protein RB2654_22 . . . 127 8e-28 gi|78773885|gb|ABB51232.1|unknown [Arthrospira platensis] 63.2 1e-08 gi|67939563|ref|ZP_00532060.1|hypothetical protein Cphamn1DR . . . 55.5 3e-06 gi|71901748|ref|ZP_00683819.1|conserved hypothetical protein . . . 53.9 8e-06 gi|9105516|gb|AAF83450.1|hypothetical protein XF_0640 [Xylel . . . 52.0 3e-05 gi|76260977|ref|ZP_00768602.1|conserved hypothetical protein . . . 51.2 5e-05 gi|53688392|ref|ZP_00345702.1|hypothetical protein Npun02002 . . . 48.9 3e-04 gi|78171892|gb|ABB28988.1|hypothetical protein Cag_1737 [Chl . . . 43.1 0.015 gi|71491000|gb|EAO23340.1|hypothetical protein SwolDRAFT_003 . . . 42.7 0.019 gi|86211168|gb|ABC87270.1|NotI restriction endonuclease [Nocard 42.0 0.033 gi|20988646|gb|AAH29858.1|Leucine rich repeat containing 23 [Ho 37.7 0.62 gi|89300041|gb|EAR98029.1|hypothetical protein TTHERM_002842 . . . 37.4 0.81

[0060] The first seven hits have E values of less than e-02, indicating that these are likely to be restriction endonucleases. Note that the sequences identified are reported as "hypothetical protein," "unknown" or "conserved hypothethical protein," since the nature of these ORFs is not known. Because the E values are not very small, with the exception of the first hit, it is more likely that these endonucleases may have somewhat different recognition sequences from EagI, and indeed may recognize novel DNA sequences. All seven of these ORFs are adjacent to an ORF identified as a methyltransferase. Interestingly, the methyltransferases are of different types, with the 8e-28 hit (gi:84686807) having a 5-methyl cytosine-methyltransferase like EagI, while the other 6 hits are adjacent to an amino-methyltransferase that could modify either N4C or N6A, which is different from the EagI R-M system. These seven ORFs can be confirmed as endonucleases by the techniques described in Example 3.

[0061] The subsequent hits are outside the cut off value of E value <e-02, however two of the next best three hits are of interest. The first such hit, cag_--1737 (gi:78171892) is not next to an identified methylase: however, there is a methylase motif, DPPY, in the sequence adjacent to cag_--1737, perhaps indicating this is a R-M system that is degenerated or partially disrupted. The next best hit, SwoIDRAFT_--0030 (gi:71491000), does have a methyltransferase adjacent to it. Even though the similarity is less than the described cut off, this gene is likely a restriction endonuclease, albeit one that is more highly diverged from EagI. The next best scoring sequence is a known restriction endonuclease, NotI. The further best scoring sequences do not appear to be restriction endonucleases. This example demonstrates that had we set the Expectation value threshold to a less stringent value, such as E<0.1, we would have included two additional true positive finds, while also including one false positive sequence (cag_--1737). Setting the cut off threshold at E less than or equal to e-02 ensures that the sequences identified are very likely to be restriction endonucleases.

[0062] When a PSI-BLAST similarity analysis was performed, the results were broadly similar to the BLAST search, but the differences demonstrated the increased sensitivity obtained using PSI-BLAST. After three iterations of PSI-BLAST, no additional new hits are obtained. The best scoring results from the fourth iteration are:

TABLE-US-00004 Score E (Bits) Value Sequences producing significant alignments: gi|76260977|ref|ZP_00768602.1|conserved hypothetical protein . . . 344 3e-93 gi|84686807|ref|ZP_01014694.1|hypothetical protein RB2654_22 . . . 337 4e-91 gi|67939563|ref|ZP_00532060.1|hypothetical protein Cphamn1DR . . . 328 3e-88 gi|78171892|gb|ABB28988.1|hypothetical protein Cag_1737 [Chl . . . 299 9e-80 gi|89055379|ref|YP_510830.1|hypothetical protein Jann_2888 [ . . . 288 2e-76 gi|86211168|gb|ABC87270.1|NotI restriction endonuclease [Nocard 286 6e-76 gi|56677682|gb|AAV94348.1|hypothetical protein SPO1048 [Sili . . . 286 1e-75 gi|71901748|ref|ZP_00683819.1|conserved hypothetical protein . . . 268 2e-70 gi|9105516|gb|AAF83450.1|hypothetical protein XF_0640 [Xylel . . . 266 8e-70 gi|70779363|gb|AAZ08143.1|ORF2 [Burkholderia cepacia] 266 1e-69 gi|78773885|gb|ABB51232.1|unknown [Arthrospira platensis] 266 1e-69 gi|53688392|ref|ZP_00345702.1|hypothetical protein Npun02002 . . . 260 6e-68 gi|71491000|gb|EAO23340.1|hypothetical protein SwolDRAFT_003 . . . 222 1e-56 gi|67935692|ref|ZP_00528711.1|hypothetical protein Cpha266DR . . . 211 3e-53 Sequences with E-value WORSE than threshold gi|3660495|emb|CAA57707.1|R.EcoHK31I protein [Escherichia coli] 43.8 0.008 gi|2760956|gb|AAB95338.1|EaeI restriction endonuclease [Enterob 42.3 0.028 gi|89300400|gb|EAR98388.1|cyclic nucleotide binding domain c . . . 39.2 0.22 gi|21355217|ref|NP_651245.1|CG5728-PA [Drosophila melanogast . . . 38.0 0.48

[0063] The seven restriction endonuclease hits identified using standard BLAST were also identified by PSI-BLAST, while the more sensitive PSI-BLAST identified seven additional restriction endonucleases, three of which were the three hits just outside of the e-02 cutoff from the standard BLAST and four of which were new to the PSI-BLAST results.

[0064] The fourteen restriction endonuclease hits form five groups of related sequences. The first group consists of those sequences that appear likely to recognize the same recognition sequence as EagI. Only three of the fourteen sequences fall into this group: hypothetical proteins RB2654_--22558, Jann_--2888 and SP01048. These three sequences are considered likely to share the EagI recognition sequence because they are adjacent to methyltransferases that are very highly similar to the EagI R-M system methyltransferase, M.EagI, with E value scores of E=0.0 using standard BLAST against the REBASE database and significant similarity through the genes, including in the region of the TRD (target recognition domain). Interestingly, only one of these sequences was identified in the EagI standard BLAST results, RB2564_--22558, where it is the highest scoring sequence. Because Jann_--2888 and SP01048 are only identified by the PSI-BLAST, it is more likely that they may have differences in functional properties with EagI.

[0065] The second grouping includes four sequences, two of which are nearly identical, being found in the same genus and species though different strains: XF_--0640 and XfasoDRAFT_--0198, along with Arthrospira platensis ORF2 and Npun02002587. This group is likely to recognize a different sequence than EagI, which demonstrates the ability of this method to identify restriction endonucleases having different recognition sequences. All four restriction endonucleases of this group are adjacent to methyltransferases that are very highly similar (E=0.0) to the BsiWI methyltransferase, and all are significantly similar to the BsiWI RE sequence (E=2e-92 to E=9e-99 when using standard BLAST at NCBI searching the Genbank database with the BsiWI probes- SEQ ID. 185 and 186). Additionally, all four have a large gap in the PSI-BLAST alignment with EagI from amino acid position 69 to 96, which is a relatively large region of difference indicating a different function for the proteins. EagI and BsiWI recognize related sequences, differing only in the internal two bases recognized: CGGCCG (EagI) and CGTACG (BsiWI). It is interesting that the sequence similarity observed implies a common ancestor for the EagI restrcition endonuclease (and family members) and the BsiWI restriction endonuclease (and these four family members).

[0066] The third grouping is characterized by: the similarity of the sequences in a standard BLAST analysis compared with other identified restriction endonuclease sequences; and the high degree of similarity of adjacent methyltransferases, while only moderately similar to methyltransferases of known specificity in a standard BLAST. This group consists of CaurDRAFT_--2301, Cphamn1DRAFT_--2148, Burkholderia cepacia ORF2, SwoIDRAFT_--0030 and Cpha266DRAFT_--1693. These five sequences are good candidates to recognize sequences different from that recognized by EagI, even though the first sequence (CaurDRAFT_--2301) scores the best in the PSI-BLAST results, because the adjacent methylases do not closely match any other methylase of known specificity, and because the restriction endonucleases have multiple small gaps in the alignments. In the case of Cpha266DRAFT_--1693, there is no adjacent methyltransferase gene. However, the C-terminal portion of the sequence has significant similarity to the methyltransferases adjacent to the other members of this group, while the N-terminal portion of this sequence matches the identified restriction endonucleases of this group. This may be a case of poor sequence data because the sequence is reported as a DRAFT, or it may be that this R-M system is degraded. The region of this gene could be amplified by PCR and sequenced to determine which is the case.

[0067] The last two groups have only one member. The first case is the cag_--1737 sequence. This sequence is significantly larger than EagI, at 498 amino acids versus 301 for EagI. Similarity to EagI occurs in the carboxy terminal half of the sequence, while the amino terminal portion does not have high similarity to EagI or to methyltransferases. There is no adjacent readily identifiable methyltransferase to be found near cag_--1737. The lack of methyltransferase and unique N-terminal half of this sequence makes it likely to be different than EagI. This would be an interesting candidate to characterize, though it is possible this may not be functioning as a typical type II restriction endonuclease.

[0068] The last group is the case of the identified NotI RE. NotI recognizes a more specific sequence, GCGGCCGC, than EagI, CGGCCG, wherein the internal six bases of the NotI recognition sequence are the same as the EagI recognition sequence. It is thus not surprising that the NotI sequence can be identified using the EagI sequence as they are likely descended from a common ancestor. Clues that the NotI gene identified might be different from EagI are that the NotI sequence is longer (383 amino acids versus 301 amino acids), that there are eight gaps in EagI sequence in the alignment with NotI, and that the methyltransferases adjacent to NotI and EagI are of different classes, wherein the M.NotI is an amino methyltransferase, while the EagI methyltransferase, M.EagI, is a 5 methyl-cytosine class methylase. Finding this restriction endonuclease having a different recognition sequence demonstrates the ability of the method to identify novel and useful new restriction endonucleases.

[0069] C. Results of searching using the Mmell amino acid sequence of FIG. 2 on 25 Jul. 2006 with the standard BLAST blastp program.

TABLE-US-00005 Score E Sequences producing significant alignments: (Bits) Value gi|67916884|ref|ZP_00510572.1|conserved hypothetical protein . . . 384 3e-105 gi|28210143|ref|NP_781087.1|hypothetical protein CTC00388 [C . . . 355 2e-96 gi|109647707|ref|ZP_01371610.1|conserved hypothetical protei . . . 348 3e-94 gi|89892981|ref|YP_516468.1|hypothetical protein DSY0235 [De . . . 344 4e-93 gi|89209832|ref|ZP_01188226.1|conserved hypothetical protein . . . 316 1e-84 gi|89201678|ref|ZP_01180415.1|conserved hypothetical protein . . . 316 1e-84 gi|110168399|gb|ABG52939.1|conserved hypothetical protein [T . . . 293 1e-77 gi|77412887|ref|ZP_00789091.1|conserved hypothetical protein . . . 259 2e-67 gi|81427752|ref|YP_394751.1|hypothetical protein [Lactobacil . . . 216 1e-54 gi|56295584|emb|CAH04826.1|conserved hypothetical protein [uncu 163 2e-38 gi|110620011|emb|CAJ35289.1|conserved hypothetical protein [ . . . 159 4e-37 gi|76260023|ref|ZP_00767665.1|hypothetical protein CaurDRAFT . . . 36.2 2.7 gi|19921772|ref|NP_610333.1|CG8728-PA [Drosophila melanogast . . . 36.2 3.2 gi|68544761|ref|ZP_00584368.1|Globin:Oxidoreductase FAD/NAD( . . . 35.4 5.5 gi|44891709|tpg|DAA02258.1|TPA: TPA_exp: S6 sporozoite-induced 35.0 6.9 gi|82594327|ref|XP_725378.1|hypothetical protein PY04986 [Pl . . . 35.0 6.9 gi|68139991|gb|EAM93304.1|Glycine hydroxymethyltransferase [ . . . 34.7 9.9

[0070] Note that the first eleven hits are highly significant, with E values ranging from 3e-105 to 4e-37. All are adjacent to DNA methyltransferases. Note the very sharp drop off in E value results between the eleven restriction endonuclease hits and the remainder of the hits, which are outside the level of significance for identifying endonucleases described herein. Also note that the endonucleases identified are reported as "hypothetical proteins" or "conserved hypothetical proteins." Without the input of the known Mmell sequence these ORFs are not identified as endonucleases. These eleven ORFs can be confirmed as endonucleases by the techniques described in Example 3.

[0071] The same search using the sequence of MmeII in the PSI-BLAST program initially identifies the same eleven genes (first iteration), but after five iterations, an additional 5 restriction endonuclease genes are found. No new sequence hits were added at the sixth iteration. The PSI-BLAST results (sixth iteration) for MmeII are:

TABLE-US-00006 Score E (Bits) Value Sequences producing significant alignments: gi|28210143|ref|NP_781087.1|hypothetical protein CTC00388 [C . . . 674 0.0 gi|67916884|ref|ZP_00510572.1|conserved hypothetical protein . . . 667 0.0 gi|89201678|ref|ZP_01180415.1|conserved hypothetical protein . . . 646 0.0 gi|89209832|ref|ZP_01188226.1|conserved hypothetical protein . . . 642 0.0 gi|110168399|gb|ABG52939.1|conserved hypothetical protein [T . . . 620 4e-176 gi|109647707|ref|ZP_01371610.1|conserved hypothetical protei . . . 617 2e-175 gi|89892981|ref|YP_516468.1|hypothetical protein DSY0235 [De . . . 607 4e-172 gi|77412887|ref|ZP_00789091.1|conserved hypothetical protein . . . 605 2e-171 gi|81427752|ref|YP_394751.1|hypothetical protein [Lactobacil . . . 564 3e-159 gi|56295584|emb|CAH04826.1|conserved hypothetical protein [uncu 530 5e-149 gi|110620011|emb|CAJ35289.1|conserved hypothetical protein [ . . . 526 9e-148 gi|16329761|ref|NP_440489.1|hypothetical protein slr1033 [Sy . . . 169 3e-40 gi|76260023|ref|ZP_00767665.1|hypothetical protein CaurDRAFT . . . 166 2e-39 gi|71143791|gb|AAZ24264.1|hypothetical protein CPS_0499 [Col . . . 164 5e-39 gi|106890764|ref|ZP_01357956.1|hypothetical protein RoseRSDR . . . 155 4e-36 gi|78195585|gb|ABB33352.1|hypothetical protein Gmet_3139 [Ge . . . 137 7e-31 Sequences with E-value WORSE than threshold gi|47565512|ref|ZP_00236553.1|hypothetical protein protein [ . . . 41.2 0.091 gi|89295649|gb|EAR93637.1|cation channel family protein [Tetrah 40.9 0.12 gi|34763083|ref|ZP_00144055.1|RNA polymerase sigma-54 factor . . . 40.5 0.16

[0072] The highest scoring hits (9e-148 and smaller E values) are the same eleven hits found by standard BLAST. There is still a dramatic drop off from E=7e-31 to the next best scoring sequence at E=0.091. The five additional sequence hits identified through PSI-BLAST all align with only the carboxy portion of MmeII (amino acids 248 to 423), so they likely differ from MmeII. Two have methyltransferases adjacent to them, gi:78195585 Gmet_--3139 and gi:71143791 CPS_--0499, although the methyltransferase next to CPS_--0499 is not labeled as a methyltransferase in the sequence annotation. The other three do not have a good adjacent methyltransferase candidate gene. These three could be restriction endonucleases like PacI or PmeI that do not have a methylase partner; they could be inactive or fragmentary restriction endonucleases; they could have a methylase partner located at some distance in their genome; or they could be false positive hits. These sixteen ORFs can be confirmed as endonucleases by the techniques described in Example 3.

[0073] Note that while BclI, EagI and MmeII identify several restriction endonuclease sequences in the Genbank database, many of the restriction endonuclease sequences of FIG. 2 currently have no matches in the database at the specified E value, indicating the very unique nature of these restriction endonuclease gene sequences. As more sequences are added to the database, however, these currently unique restriction endonuclease sequences may be used to identify restriction endonucleases with which they share significant sequence similarity.

Example 3

Method of Determining Whether a Sequence Identified According to Example I has Endonuclease Activity

[0074] Various methods can be employed to determine whether a DNA sequence identified as a potential endonuclease gene encodes an active endonuclease. Several such methods are described herein.

[0075] (a) In vitro transcription and translation of the identified ORF may be used to produce the protein encoded by that identified ORF (see for example, U.S. Pat. No. 6,689,573 U.S. Pat. No. 6,905,837, U.S. Pat. No. 6,383,770). The protein thus produced is then used in standard restriction endonuclease assays to see if it cleaves DNA. If DNA cleavage is observed, the restriction endonuclease and position of DNA strand breakage within or near the recognition sequence is determined by the methods described below. In vitro transcription-translation has an advantage that there is no requirement for host DNA protection by the companion DNA methyltransferase in order to express the endonuclease, as is usually the case when cloning the restriction endonuclease into a naive host, such as typical laboratory E. coli strains. In vitro transcription and translation systems that have minimal endogenous, non-specific nucleases are preferable, such as the rabbit reticulocyte system.

[0076] (b) The candidate genes can be cloned and expressed in a host cell. E. coli is often used as a host for such cloning, but any host cell with a genetic transformation system could be used as well, such as Bacillus subtilis or yeast strains. Typically, the methyltransferase gene for the potential restriction system is cloned into a vector that has an origin of replication compatible with a second vector, which will be used for the endonuclease gene. The methyltransferase gene may be amplified by PCR using suitable primers, which primers often include a termination codon to end expression of the vector protein into which the methyltransferase gene is being inserted, a ribosome binding sequence to allow initiation of translation at the start of the methyltransferase ORF, and restriction sites to facilitate cloning. The methyltransferase gene can then be introduced into the host. Host cells expressing the methyltransferase are then grown and made competent.

[0077] If the potential endonuclease being cloned is an isoschizomer or a neoschizomer of a known enzyme, the known endonuclease can be used to test whether expression of the newly identified potential methyltransferase prevents cleavage by the known endonuclease. DNA from the methyltransferase expressing host, either the vector and methyltransferase gene or total host DNA, can be purified and digested with the known endonuclease. If the potential methyltransferase methylates the same recognition sequence as the known endonuclease, it will prevent cleavage by the known endonuclease if the methylation occurs at the same base position as methylated by the known restriction system methyltransferase.

[0078] It may be, however, that the potential methyltransferase modifies the same DNA sequence at a different position, i.e., either a different base within the recognition sequence, or at a different position on the same base, such as 5-methyl cytosine versus N4-methyl cytosine. If the base position modified is different, it may be that the potential methyltransferase modifies the host DNA but fails to protect against cleavage by the known endonuclease. This is more likely in the case of neoschizomers but can also occur with isoschizomers. Differences in methylation sensitivity can lead to new and potentially advantageous uses for isoschizomers or neoschizomers identified by the method of this application. If the potential restriction system being investigated has a different recognition sequence than the known endonuclease, it is likely that the cloned potential methyltransferase gene will not prevent cleavage by the known endonuclease. An exception to this would be if the known restriction system recognizes a sequence that is a subset of that recognized by potential restriction system. For example, MjaIV, recognizing GTNNAC, was observed to have weak similarity to HincII, recognizing GTYRAC. While MjaIV has a different recognition sequence to HincII, all HincII recognition sites are also MjaIV recognition sites, so MjaIV methylation will block HincII cutting even though MjaIV has a different recognition sequence. Once the methylase is introduced and expressed so as to protect the host cell, the endonuclease can then be introduced into those cells.

[0079] For cloning, the endonuclease gene is amplified by PCR using suitable primers, as for the methyltransferase, inserted into a vector compatible in the same host cell as the vector encoding the cognate methyltransferase, and transformed into the competent host cells already expressing the methyltransferase. Transformed host cells are then grown and a protein extract is made by appropriate methods, such as sonication or pressure disruption (french press). The protein extract is then assayed for restriction endonuclease activity by mixing various dilutions of the protein extract with standard DNAs, for example phage lambda DNA, phage T7 DNA, phage PhiX174 DNA, pBR322 DNA or pUC19 DNA, in a standard buffer, such as NEBuffer 2 or NEBuffer 4 (New England Biolabs, Inc., Ipswich, Mass.). The reaction products are electrophoresed on agarose gels and examined to see if the DNA has been cleaved.

[0080] The specific DNA sequence recognized by the endonuclease (the `recognition sequence`), and the exact position of strand cleavage within or near the recognition sequence, are then determined for the endonuclease (see Schildkraut, I. S., "Screening for and Characterizing Restriction Endonucleases", in Genetic Engineering, Principles and Methods, Vol. 6, pp. 117-140, Plenum Press (1984)), U.S. Pat. No. 5,030,569, U.S. Pat. No. 5,200,337, U.S. Pat. No. 5,192,676, U.S. Pat. No. 5,100,793, U.S. Pat. No. 4,996,151, U.S. Pat. No. 5,543,308, U.S. Pat. No. 4,999,293, U.S. Pat. No. 5,139,942, U.S. Pat. No. 5,371,006, U.S. Pat. No. 5,098,839, U.S. Pat. No. 5,196,330, U.S. Pat. No. 5,824,529, U.S. Pat. No. 5,288,696, U.S. Pat. No. 5,945,326).

[0081] For example, to determine the recognition sequence of the endonuclease, the size of the fragments generated from digestion of several of the known standard DNAs, such as lambda DNA or PhiX174 DNA, can be estimated by comparison of the fragments produced by the unknown enzyme to DNA molecular weight standards (such as NEB catalog #N3012 lambda DNA-HindIII digest, NEB cat #N3026 PhiX174 DNA-HaeIII digest, NEB cat #N3232 1 kb DNA Ladder or other DNA size standards (New England Biolabs, Inc., Ipswich, Mass.)). The approximate sizes of the fragments can be entered into a program such as REBpredictor (http://tools.neb.com/REBpredictor/index.php), which will generate possible recognition sequences matching the fragment size input data. The output of this program is usually a list of multiple possible recognition sequences, only one of which is correct. The correct recognition sequence can be determined by mapping several positions at which the unknown endonuclease cuts the standard DNAs. Mapping is done by standard techniques in which the DNA is cut with the unknown enzyme and a known endonuclease. By using several known endonucleases, which cut at various positions in the DNA, the position(s) of cleavage of the unknown enzyme can be estimated. The correct recognition sequence predicted by a program such as REBpredictor will occur at the positions determined by the mapping experiment. When a candidate recognition sequence is determined, it can be confirmed by comparing the observed sizes of DNA fragments generated by cutting with the unknown endonuclease on various DNAs to the computer predicted sizes of fragments generated in silico by cutting at the predicted recognition sequence (for example, see U.S. Pat. No. 5,030,569, U.S. Pat. No. 5,200,337, U.S. Pat. No. 5,192,676, U.S. Pat. No. 5,100,793, U.S. Pat. No. 4,996,151, U.S. Pat. No. 5,543,308, U.S. Pat. No. 4,999,293, U.S. Pat. No. 5,139,942, U.S. Pat. No. 5,371,006, U.S. Pat. No. 5,098,839, U.S. Pat. No. 5,196,330, U.S. Pat. No. 5,824,529, U.S. Pat. No. 5,288,696, U.S. Pat. No. 5,945,326).

[0082] The position of DNA strand cleavage relative to the recognition sequence can be determined by standards methods, such as that of cleavage of a primer extension product, which can then be electrophoresed alongside a set of standard dideoxy sequencing reactions produced from the same primer and template. Such an approach is exemplified in Example II of U.S. Pat. No. 6,194,188. Alternatively, a DNA can be cut by the endonuclease and then a standard sequencing reaction is performed using a primer located within 500 bases of the endonuclease-cutting site. The sequencing reaction will give normal sequence until it runs off the end of the template at the site of the endonuclease cut. Sequencing must be performed in both directions to observe the cleavage position of each DNA strand. Such an approach is exemplified in Example II of U.S. application Ser. No. 10/617,361.

[0083] The biochemical characteristics of the new restriction endonucleases identified by this method can be determined by standard techniques. Properties to be examined would include thermostability, activity in various buffer conditions, level of star activity and sensitivity to methylation at various positions within the recognition sequence. Endonucleases that differ from previously known isoschizomers in some or other of these biochemical characteristics may have uses that differ from the previously known endonucleases.

Sequence CWU 1

1

22011590DNAArthrobacter citreus 1atgttcatga atgaacacat aaagggatct aattcgcatg gtaatagcaa tgagttggaa 60ttggtttatg cgtttgatgg caagaaagtt aaggatttaa acactaattt aaaaaatttt 120gtacaattca ttgcaaacga taacaatata aaaattaata atgatacaaa attatttgcg 180aagtatgttt ctaataacaa attaaaacaa gattttattg tatcgtttaa tgaaagagat 240ttttacatta gtttaaaaat gggttcagga aatagtgttc atcaagaacc gattgaagat 300tttattaaat acttgaatac gaattatgaa gtaactgaga aaatttgtaa tgatttaagg 360ttttttattt gggcagatgg tacgttagat ggaaaaggaa aatttgagaa tagatttgac 420gctagatatt ttaaaaagaa ttatcctgaa aaacgaagag agttattaca atttttcgaa 480aaaaataagg tagaactaat taagcatttt atgtttgttg gtaaacataa tagtagagtt 540gattatcttt atcatggaac gacttcgaat ggggcgtgga tgtcaacaaa acaaataatt 600gattacaata ttcaaaacca aatagatact aacaaaggta attctcctac tttaagcgtc 660ggaagaatgt caattcaagc atggaatgtt gcaaagtctg gaagcgagtc agcagaaaag 720aaacgaggag aaatccaggt aaaatacgga aaacttaaag aagattttaa ggaggtatta 780aaattaaatt cctcgaacaa aggaactttg tttggagatc atgaggaatt tgatatttct 840ggaactttaa ataaaaataa aaatcatttt tattggaaga tgatagcaag agatttgaat 900ttaaatcaag aagaactgaa taatttatat gttgttcgtg tttcttcaaa agttatgtca 960tctctcagta agaaaaaggt tcttccaaaa tcggatgctt atataattag agctgatctc 1020tctaaatctt ttttactatc taaagaatac aaactatctg aagatgattt ggtaggtata 1080atatataaaa aagtaggacg tagtggcatt tcagtaaaaa gagctgattc gaagaaatat 1140actattgtaa aacttacagt tgcttcattt gaaaagtgct ttgaaatcga gccagagata 1200aaaaaaataa ttgctggact tttgttatat agtaaagaga aggatatgta taaaaatctt 1260gaaattttaa ataagattgg tatatctgaa cttgaattga taaattatac aaatcgattt 1320attgtagata aagttatttc gtgcaatgat cccaaaaatg tcgatataat caggagtacg 1380atgcaagaac ggactcgaac actaattgaa aaaaatttag aaatcaaaaa agcactattt 1440atgggtgaag gctggtatga agaaccctat tgcatcaatt atatttttaa agatgggaaa 1500ttatcaaatg atgtattttc tgagtatatt attactacag gttcaggccg ttcaaagggc 1560aattatacaa ttgcgttgaa gcctaagtaa 15902529PRTArthrobacter citreus 2Met Phe Met Asn Glu His Ile Lys Gly Ser Asn Ser His Gly Asn Ser 1 5 10 15 Asn Glu Leu Glu Leu Val Tyr Ala Phe Asp Gly Lys Lys Val Lys Asp 20 25 30 Leu Asn Thr Asn Leu Lys Asn Phe Val Gln Phe Ile Ala Asn Asp Asn 35 40 45 Asn Ile Lys Ile Asn Asn Asp Thr Lys Leu Phe Ala Lys Tyr Val Ser 50 55 60 Asn Asn Lys Leu Lys Gln Asp Phe Ile Val Ser Phe Asn Glu Arg Asp 65 70 75 80 Phe Tyr Ile Ser Leu Lys Met Gly Ser Gly Asn Ser Val His Gln Glu 85 90 95 Pro Ile Glu Asp Phe Ile Lys Tyr Leu Asn Thr Asn Tyr Glu Val Thr 100 105 110 Glu Lys Ile Cys Asn Asp Leu Arg Phe Phe Ile Trp Ala Asp Gly Thr 115 120 125 Leu Asp Gly Lys Gly Lys Phe Glu Asn Arg Phe Asp Ala Arg Tyr Phe 130 135 140 Lys Lys Asn Tyr Pro Glu Lys Arg Arg Glu Leu Leu Gln Phe Phe Glu 145 150 155 160 Lys Asn Lys Val Glu Leu Ile Lys His Phe Met Phe Val Gly Lys His 165 170 175 Asn Ser Arg Val Asp Tyr Leu Tyr His Gly Thr Thr Ser Asn Gly Ala 180 185 190 Trp Met Ser Thr Lys Gln Ile Ile Asp Tyr Asn Ile Gln Asn Gln Ile 195 200 205 Asp Thr Asn Lys Gly Asn Ser Pro Thr Leu Ser Val Gly Arg Met Ser 210 215 220 Ile Gln Ala Trp Asn Val Ala Lys Ser Gly Ser Glu Ser Ala Glu Lys 225 230 235 240 Lys Arg Gly Glu Ile Gln Val Lys Tyr Gly Lys Leu Lys Glu Asp Phe 245 250 255 Lys Glu Val Leu Lys Leu Asn Ser Ser Asn Lys Gly Thr Leu Phe Gly 260 265 270 Asp His Glu Glu Phe Asp Ile Ser Gly Thr Leu Asn Lys Asn Lys Asn 275 280 285 His Phe Tyr Trp Lys Met Ile Ala Arg Asp Leu Asn Leu Asn Gln Glu 290 295 300 Glu Leu Asn Asn Leu Tyr Val Val Arg Val Ser Ser Lys Val Met Ser 305 310 315 320 Ser Leu Ser Lys Lys Lys Val Leu Pro Lys Ser Asp Ala Tyr Ile Ile 325 330 335 Arg Ala Asp Leu Ser Lys Ser Phe Leu Leu Ser Lys Glu Tyr Lys Leu 340 345 350 Ser Glu Asp Asp Leu Val Gly Ile Ile Tyr Lys Lys Val Gly Arg Ser 355 360 365 Gly Ile Ser Val Lys Arg Ala Asp Ser Lys Lys Tyr Thr Ile Val Lys 370 375 380 Leu Thr Val Ala Ser Phe Glu Lys Cys Phe Glu Ile Glu Pro Glu Ile 385 390 395 400 Lys Lys Ile Ile Ala Gly Leu Leu Leu Tyr Ser Lys Glu Lys Asp Met 405 410 415 Tyr Lys Asn Leu Glu Ile Leu Asn Lys Ile Gly Ile Ser Glu Leu Glu 420 425 430 Leu Ile Asn Tyr Thr Asn Arg Phe Ile Val Asp Lys Val Ile Ser Cys 435 440 445 Asn Asp Pro Lys Asn Val Asp Ile Ile Arg Ser Thr Met Gln Glu Arg 450 455 460 Thr Arg Thr Leu Ile Glu Lys Asn Leu Glu Ile Lys Lys Ala Leu Phe 465 470 475 480 Met Gly Glu Gly Trp Tyr Glu Glu Pro Tyr Cys Ile Asn Tyr Ile Phe 485 490 495 Lys Asp Gly Lys Leu Ser Asn Asp Val Phe Ser Glu Tyr Ile Ile Thr 500 505 510 Thr Gly Ser Gly Arg Ser Lys Gly Asn Tyr Thr Ile Ala Leu Lys Pro 515 520 525 Lys 3972DNAAcinetobacter calcoaceticus M4 3ttgggaaaac ttaaaaaaat tttagcgggt actgctggtc gaaatactgg tcatgcatat 60gaagaatatc tagcagataa tattaattca ttaaatgttc cattaaatat atcaatacaa 120ccactaccga tagtaggttc ttatcaaaat gtcttttctt caaataatga agctttcaca 180ttaatcactt atttggctaa agcatataat atttctcaaa ttactagaat ttatgctgtt 240gcaacagggg gattagcaac atccgctaac agtcagggtg tccattttaa tggacaaata 300attaaaaaat gtaaaagtga tattttaatt gaaatcgaag accaatctaa tcaaattcat 360cgtattggga tatctgtaaa acaatgtaac aatcagagac ctcttaatgc tcaagttttt 420tgttccacag cttcagggtt ttctaaccta ttaagacaac ataacattcc agtttctatt 480ttagctgaaa atgaattacg taaattctgt ggtcatcctg gaagctctcc aatagatcac 540aatattcatt tgacccgtgt aattgatcat agaagatact tttgggaaga actagatatt 600aatgcatcac aggagtggca aaatattttt gataattaca acgctgaaat agctagaatt 660ttattgcaaa aagcatatag taacgaacct tatccacctg agtttttatt acataaaaca 720aagagaacct caggaaatca ggaaattgct atttttgaaa ttgctgattt aatagtaaaa 780agttttcagt atcaatgctt tacaacatca ctctatagag taaaaaaagg aacttttaaa 840gaagctaaaa ctatgggaga tacacacgag gctcctagtt ttggaatatt tcaaatgcaa 900cgcttaggaa atactcaaaa tgcacatcaa ttgcaattta atttaaaagc cggctatttt 960tatcatattt aa 9724323PRTAcinetobacter calcoaceticus M4 4Met Gly Lys Leu Lys Lys Ile Leu Ala Gly Thr Ala Gly Arg Asn Thr 1 5 10 15 Gly His Ala Tyr Glu Glu Tyr Leu Ala Asp Asn Ile Asn Ser Leu Asn 20 25 30 Val Pro Leu Asn Ile Ser Ile Gln Pro Leu Pro Ile Val Gly Ser Tyr 35 40 45 Gln Asn Val Phe Ser Ser Asn Asn Glu Ala Phe Thr Leu Ile Thr Tyr 50 55 60 Leu Ala Lys Ala Tyr Asn Ile Ser Gln Ile Thr Arg Ile Tyr Ala Val 65 70 75 80 Ala Thr Gly Gly Leu Ala Thr Ser Ala Asn Ser Gln Gly Val His Phe 85 90 95 Asn Gly Gln Ile Ile Lys Lys Cys Lys Ser Asp Ile Leu Ile Glu Ile 100 105 110 Glu Asp Gln Ser Asn Gln Ile His Arg Ile Gly Ile Ser Val Lys Gln 115 120 125 Cys Asn Asn Gln Arg Pro Leu Asn Ala Gln Val Phe Cys Ser Thr Ala 130 135 140 Ser Gly Phe Ser Asn Leu Leu Arg Gln His Asn Ile Pro Val Ser Ile 145 150 155 160 Leu Ala Glu Asn Glu Leu Arg Lys Phe Cys Gly His Pro Gly Ser Ser 165 170 175 Pro Ile Asp His Asn Ile His Leu Thr Arg Val Ile Asp His Arg Arg 180 185 190 Tyr Phe Trp Glu Glu Leu Asp Ile Asn Ala Ser Gln Glu Trp Gln Asn 195 200 205 Ile Phe Asp Asn Tyr Asn Ala Glu Ile Ala Arg Ile Leu Leu Gln Lys 210 215 220 Ala Tyr Ser Asn Glu Pro Tyr Pro Pro Glu Phe Leu Leu His Lys Thr 225 230 235 240 Lys Arg Thr Ser Gly Asn Gln Glu Ile Ala Ile Phe Glu Ile Ala Asp 245 250 255 Leu Ile Val Lys Ser Phe Gln Tyr Gln Cys Phe Thr Thr Ser Leu Tyr 260 265 270 Arg Val Lys Lys Gly Thr Phe Lys Glu Ala Lys Thr Met Gly Asp Thr 275 280 285 His Glu Ala Pro Ser Phe Gly Ile Phe Gln Met Gln Arg Leu Gly Asn 290 295 300 Thr Gln Asn Ala His Gln Leu Gln Phe Asn Leu Lys Ala Gly Tyr Phe 305 310 315 320 Tyr His Ile 5933DNAAnabaena flos-aquae 5atgacatttc aaataactcc agaaaatacc caaaataaat ctgctttgtt ggaatatttt 60agagaattag gaaatgaaaa actttctgaa attagaagag aagtcggtaa taaaaattat 120caaaaaataa ctcctcatat caataaagca atcaataaaa ctaagaatga ttttttacat 180acaattattg aaactgcaaa tcaaaataat tggaatgatc aagataaact ttcctcactt 240cttttcacta cttattgcgc tcatgtagta atgcttgatt tacgtcatga agtgtggcct 300tatgaatata tggcattttc tcgacgaatt ggtgagcttt gggaaaattt tgtaagatta 360ccatttttgt acccgccaaa agcagcagaa ttaacctctt ttgtaccacc acttttttct 420gaagtcagaa aaaatctaaa aaaagatatt gaagaatata ttgatacttt gttcatttct 480caagaacaaa aatatgaact tatcaattat tatgaaaaag tatgggtgct agtagattct 540ggtgaaataa gtcttgagtt agattttcat gctattatta gaggtaaaag attcaatata 600gattttaaaa gtggatttgg ctcaaatgag aaaggaaata ccaaccgact gcttatggta 660gcaactattt attgtaattt agaagatgaa tatagtaata ttctgctcgt tcgcgctaaa 720gaagatttga ataacaatta ctttagaact ttaaaaaagt ctcatgtttg gaatgcatat 780tgtggtgatg aagcttatga aaaaattggt gattttacaa attttgatat gcgaacctgg 840ataaattcaa gtattgattg gcaaaatgat ttattaagta caacagtaag tgattttgag 900aaatttaatt taatgggata tttacaatgg taa 9336310PRTAnabaena flos-aquae 6Met Thr Phe Gln Ile Thr Pro Glu Asn Thr Gln Asn Lys Ser Ala Leu 1 5 10 15 Leu Glu Tyr Phe Arg Glu Leu Gly Asn Glu Lys Leu Ser Glu Ile Arg 20 25 30 Arg Glu Val Gly Asn Lys Asn Tyr Gln Lys Ile Thr Pro His Ile Asn 35 40 45 Lys Ala Ile Asn Lys Thr Lys Asn Asp Phe Leu His Thr Ile Ile Glu 50 55 60 Thr Ala Asn Gln Asn Asn Trp Asn Asp Gln Asp Lys Leu Ser Ser Leu 65 70 75 80 Leu Phe Thr Thr Tyr Cys Ala His Val Val Met Leu Asp Leu Arg His 85 90 95 Glu Val Trp Pro Tyr Glu Tyr Met Ala Phe Ser Arg Arg Ile Gly Glu 100 105 110 Leu Trp Glu Asn Phe Val Arg Leu Pro Phe Leu Tyr Pro Pro Lys Ala 115 120 125 Ala Glu Leu Thr Ser Phe Val Pro Pro Leu Phe Ser Glu Val Arg Lys 130 135 140 Asn Leu Lys Lys Asp Ile Glu Glu Tyr Ile Asp Thr Leu Phe Ile Ser 145 150 155 160 Gln Glu Gln Lys Tyr Glu Leu Ile Asn Tyr Tyr Glu Lys Val Trp Val 165 170 175 Leu Val Asp Ser Gly Glu Ile Ser Leu Glu Leu Asp Phe His Ala Ile 180 185 190 Ile Arg Gly Lys Arg Phe Asn Ile Asp Phe Lys Ser Gly Phe Gly Ser 195 200 205 Asn Glu Lys Gly Asn Thr Asn Arg Leu Leu Met Val Ala Thr Ile Tyr 210 215 220 Cys Asn Leu Glu Asp Glu Tyr Ser Asn Ile Leu Leu Val Arg Ala Lys 225 230 235 240 Glu Asp Leu Asn Asn Asn Tyr Phe Arg Thr Leu Lys Lys Ser His Val 245 250 255 Trp Asn Ala Tyr Cys Gly Asp Glu Ala Tyr Glu Lys Ile Gly Asp Phe 260 265 270 Thr Asn Phe Asp Met Arg Thr Trp Ile Asn Ser Ser Ile Asp Trp Gln 275 280 285 Asn Asp Leu Leu Ser Thr Thr Val Ser Asp Phe Glu Lys Phe Asn Leu 290 295 300 Met Gly Tyr Leu Gln Trp 305 310 7702DNAAnabaena flos-aquae 7atgataaatg aagatgattt actgaatata gctatagtaa atattagaaa agtttcaaaa 60tttaaacctt ataaaagcta ttctggtgtc aataacaaag aggaatttca gcaattaata 120gctaatgatc ctgcttttgg ctctttaggt ttggacgacg aaagatacat tattgccaga 180gtgggaggaa atcttgtaac ctccttacat cgcaaacttg gtgatatgta tgagaattta 240tttgcctatt tattaaaaga gagttttgga ttaaatgaga atgaactaca ctttagtgtc 300aatgttaaaa ttggtaaacg tgaccaggtt cgatccactg atggattaat cagaaaagat 360aagttcaatc aaaatattcc ctcagattgg attaaatatg aaggaatagg atttgaagtc 420cgttcatgct atcaaattgg tgattctaaa agaattcagg ctgattatga tatgtccttg 480gctttgaagt cttacgaaat tctgcctgta atgttaattt tctgcaatac atctttaaaa 540agccctgtac ttagattatc aaagagttgg gaactctatg aaggaagaaa cagttttgat 600ttagttcata ctattactgg tttcgacctt tataatttcc ttcagataaa ttcagagttg 660ttgaaaaaag agatagataa tattttctca tattttctct aa 7028233PRTAnabaena flos-aquae 8Met Ile Asn Glu Asp Asp Leu Leu Asn Ile Ala Ile Val Asn Ile Arg 1 5 10 15 Lys Val Ser Lys Phe Lys Pro Tyr Lys Ser Tyr Ser Gly Val Asn Asn 20 25 30 Lys Glu Glu Phe Gln Gln Leu Ile Ala Asn Asp Pro Ala Phe Gly Ser 35 40 45 Leu Gly Leu Asp Asp Glu Arg Tyr Ile Ile Ala Arg Val Gly Gly Asn 50 55 60 Leu Val Thr Ser Leu His Arg Lys Leu Gly Asp Met Tyr Glu Asn Leu 65 70 75 80 Phe Ala Tyr Leu Leu Lys Glu Ser Phe Gly Leu Asn Glu Asn Glu Leu 85 90 95 His Phe Ser Val Asn Val Lys Ile Gly Lys Arg Asp Gln Val Arg Ser 100 105 110 Thr Asp Gly Leu Ile Arg Lys Asp Lys Phe Asn Gln Asn Ile Pro Ser 115 120 125 Asp Trp Ile Lys Tyr Glu Gly Ile Gly Phe Glu Val Arg Ser Cys Tyr 130 135 140 Gln Ile Gly Asp Ser Lys Arg Ile Gln Ala Asp Tyr Asp Met Ser Leu 145 150 155 160 Ala Leu Lys Ser Tyr Glu Ile Leu Pro Val Met Leu Ile Phe Cys Asn 165 170 175 Thr Ser Leu Lys Ser Pro Val Leu Arg Leu Ser Lys Ser Trp Glu Leu 180 185 190 Tyr Glu Gly Arg Asn Ser Phe Asp Leu Val His Thr Ile Thr Gly Phe 195 200 205 Asp Leu Tyr Asn Phe Leu Gln Ile Asn Ser Glu Leu Leu Lys Lys Glu 210 215 220 Ile Asp Asn Ile Phe Ser Tyr Phe Leu 225 230 91068DNAAcetobacter pasteurianus sub. pasteurianus 9atggcccgca acgtgttagt tgaacgtgcg gtggacgctg cgcttgaacg gctggacgcc 60ttcattgaag gcgagaagct ggcaaagttg cccgatgctg cgacccgagc attactggac 120gaccagcttg gccacggatc taacagcgtt cgattagcat cgctgttctt tgtcttctat 180gcgtcagttg atctggcatg ggattgcaat tcaatcccga ccggaattcg cggcacctac 240ggcgacaagc ggttggcgac gcaacttggg cttcgtagca tcacgcttca caatgccatt 300acggccttcg gagaaaacct tggctggaaa ggaaacgtta ctaattcgcg ccttcagaac 360gacgtgcgat tcgacggatt tggccgaact cttactggcc tgaacgtcga acagcggacg 420ctatgcgctg actatatggc cgcacggttt gccgaaagcc ggaaagtcat tgcaccgtta 480ccaccagtcg ccgatgacgt gttgacctac gcccgtgcgc ggaagctgtt ctattcactg 540atcgcccttc catccgaagg taacgttcag caattcctta tcgccgcact gttgttcgtt 600catcgccagc gatacggtta tgacattcga acccatcacg ttcatgcttc agaccgcttc 660gatacgacag cgggcgatat tgaggaactg ctgaacggcg accttgtgcg cgcctacgaa 720gtgacagttc gacccgattg gaagaaccgc atgggcgact tccgaaaaaa gatggatggt 780gcgagccttc gcaaatacac catcattgcg tcgaacgtga acagcgacga cgatcttgcc 840gaacccgccg acatgatccg cttccttacg ccctatggcc gcgacattgc gatcgtggat 900attcacgatt tcatcaacgt gttcgcaatg gaattgatcg tcgacgaact gtgtcgggct 960gtcacgcaaa cctacaacta tcttacaacg ccaagcttgt gcggacgtgc tgatattatc 1020gataagttca acgctaccgt cgaaggttgg ttggatgaag taacctaa 106810355PRTAcetobacter pasteurianus sub. pasteurianus 10Met Ala Arg Asn Val Leu Val Glu Arg Ala Val Asp Ala Ala Leu Glu 1 5 10 15 Arg Leu Asp Ala Phe Ile Glu Gly Glu Lys Leu Ala Lys Leu Pro Asp 20 25 30 Ala Ala Thr Arg

Ala Leu Leu Asp Asp Gln Leu Gly His Gly Ser Asn 35 40 45 Ser Val Arg Leu Ala Ser Leu Phe Phe Val Phe Tyr Ala Ser Val Asp 50 55 60 Leu Ala Trp Asp Cys Asn Ser Ile Pro Thr Gly Ile Arg Gly Thr Tyr 65 70 75 80 Gly Asp Lys Arg Leu Ala Thr Gln Leu Gly Leu Arg Ser Ile Thr Leu 85 90 95 His Asn Ala Ile Thr Ala Phe Gly Glu Asn Leu Gly Trp Lys Gly Asn 100 105 110 Val Thr Asn Ser Arg Leu Gln Asn Asp Val Arg Phe Asp Gly Phe Gly 115 120 125 Arg Thr Leu Thr Gly Leu Asn Val Glu Gln Arg Thr Leu Cys Ala Asp 130 135 140 Tyr Met Ala Ala Arg Phe Ala Glu Ser Arg Lys Val Ile Ala Pro Leu 145 150 155 160 Pro Pro Val Ala Asp Asp Val Leu Thr Tyr Ala Arg Ala Arg Lys Leu 165 170 175 Phe Tyr Ser Leu Ile Ala Leu Pro Ser Glu Gly Asn Val Gln Gln Phe 180 185 190 Leu Ile Ala Ala Leu Leu Phe Val His Arg Gln Arg Tyr Gly Tyr Asp 195 200 205 Ile Arg Thr His His Val His Ala Ser Asp Arg Phe Asp Thr Thr Ala 210 215 220 Gly Asp Ile Glu Glu Leu Leu Asn Gly Asp Leu Val Arg Ala Tyr Glu 225 230 235 240 Val Thr Val Arg Pro Asp Trp Lys Asn Arg Met Gly Asp Phe Arg Lys 245 250 255 Lys Met Asp Gly Ala Ser Leu Arg Lys Tyr Thr Ile Ile Ala Ser Asn 260 265 270 Val Asn Ser Asp Asp Asp Leu Ala Glu Pro Ala Asp Met Ile Arg Phe 275 280 285 Leu Thr Pro Tyr Gly Arg Asp Ile Ala Ile Val Asp Ile His Asp Phe 290 295 300 Ile Asn Val Phe Ala Met Glu Leu Ile Val Asp Glu Leu Cys Arg Ala 305 310 315 320 Val Thr Gln Thr Tyr Asn Tyr Leu Thr Thr Pro Ser Leu Cys Gly Arg 325 330 335 Ala Asp Ile Ile Asp Lys Phe Asn Ala Thr Val Glu Gly Trp Leu Asp 340 345 350 Glu Val Thr 355 11714DNAArthrobacter protophormiae 11atggcgcaga aggcacgact tcggcagaac cgctacggga ctgtcatcaa tacgacctcg 60tctaagcagg agctgcagct cggtgacgcg cttgtcgatg ccaccgagcg ccttacggcg 120aagttcggta tcgccttcac gcacgagaag aaggtgatgc tcgctgatat cgtcacctcc 180ctccgccgca gcttcccgac ggtgtcgttc gatgacccgc tcccgaacac ttacatgagc 240cccgacggcg gcatcctctc aatcatggcg gcggacggcg agcgcacatt ccctgtactg 300atcacggagg tgaagaacca ggggaccaac gacctgcggg ctcaggaggg gctgaagaag 360caagcgatgg gtaatgccat cgagcgcctc gggaagaacg tgatcggatt ccgcgcaatg 420atgctggagg acggaatcat cccgttcgtg tgctttggct acggctggga tttccacgag 480ggtagttcga ttctcgacag ggtgaagacc atcgctatgt tcggcgagct gaatcaggtg 540aacgtcatcc ctgaagggga ggaggggctc ttcaatcgag gcagcttctt cttccggatg 600gagccttggt ccttggaaga gatgtcggat gtgatgtttg acgtcgggag ccgtgcgatt 660cactactact tcgctaagtt cggcgattct gcgttcaaaa tgattggttc ctaa 71412237PRTArthrobacter protophormiae 12Met Ala Gln Lys Ala Arg Leu Arg Gln Asn Arg Tyr Gly Thr Val Ile 1 5 10 15 Asn Thr Thr Ser Ser Lys Gln Glu Leu Gln Leu Gly Asp Ala Leu Val 20 25 30 Asp Ala Thr Glu Arg Leu Thr Ala Lys Phe Gly Ile Ala Phe Thr His 35 40 45 Glu Lys Lys Val Met Leu Ala Asp Ile Val Thr Ser Leu Arg Arg Ser 50 55 60 Phe Pro Thr Val Ser Phe Asp Asp Pro Leu Pro Asn Thr Tyr Met Ser 65 70 75 80 Pro Asp Gly Gly Ile Leu Ser Ile Met Ala Ala Asp Gly Glu Arg Thr 85 90 95 Phe Pro Val Leu Ile Thr Glu Val Lys Asn Gln Gly Thr Asn Asp Leu 100 105 110 Arg Ala Gln Glu Gly Leu Lys Lys Gln Ala Met Gly Asn Ala Ile Glu 115 120 125 Arg Leu Gly Lys Asn Val Ile Gly Phe Arg Ala Met Met Leu Glu Asp 130 135 140 Gly Ile Ile Pro Phe Val Cys Phe Gly Tyr Gly Trp Asp Phe His Glu 145 150 155 160 Gly Ser Ser Ile Leu Asp Arg Val Lys Thr Ile Ala Met Phe Gly Glu 165 170 175 Leu Asn Gln Val Asn Val Ile Pro Glu Gly Glu Glu Gly Leu Phe Asn 180 185 190 Arg Gly Ser Phe Phe Phe Arg Met Glu Pro Trp Ser Leu Glu Glu Met 195 200 205 Ser Asp Val Met Phe Asp Val Gly Ser Arg Ala Ile His Tyr Tyr Phe 210 215 220 Ala Lys Phe Gly Asp Ser Ala Phe Lys Met Ile Gly Ser 225 230 235 131368DNAArthrobacter species 13gtgattgaat ttcccgagta tcgagacagc tcggccgcgc caaagatttc agaccttgaa 60aggctgatgg atcgctcctt gaccaactcg cagtacgtgg atggggcgaa cgcagctcgc 120ttgctaggca catttatccg gtcatttcgt agcgtcattg gtagcgccga ggagtcggcc 180actcgagcca atttagttga ggcgcacgac gaggcgaaac tcttcgggtt gatgctatcc 240gcgggattcg acctaatttg taatgcagaa tatgtccacg ggcgattggt caacaataag 300tggatctact gccaccgagg gggtgagccg gctgtcgcct attactcttt cctgaaacag 360tgtcctcggt gctgccttga ccgaggcttg gaggggcggc tgagtggcgc acagcacaag 420ccgacgagcc atcacatcgg tgagataacc accgtcgcga tcgcccttct tctccagttg 480gtggctgccg ctaacgagaa tccgttcgaa atcgccacga tcacaaaaca gtcgcatgac 540gttgatgcca ttggcttccg tgacgatctt ttggtccttt tcgagattaa agcttcccca 600atggtctcct ttcctctggt gactgaactg gaagaaccaa tgctgcagga gggccccgac 660ggcccagtag agtaccggca acactcattg gtggatttaa cgctgcaagg ccgagaattc 720gctgtagcta tcccgcacgc agagacggca atccctctcg gtgaacgaga gggggagtct 780tggccctatg aaccgctgat cgactacttc tcagtacccg ccaatgccgc tagttatctg 840caggcgtgga tcgagctgta cgcggcttac agaaccccca agacgcaaag agcgggccgg 900actgctgcgc tcgcgtatct cgtgaacggc tggggtgacg agatcgactc caataagact 960aagcctgggc ttgggcggac tgatgacgta aaaaaaggca cttatcagtt gttgaagttc 1020ggttcctact atagggacga cgccgcaagt gtgcctgtaa ggggtgctct ggtcgccaac 1080cttgatccgc tctttctgcg ccctggttac atcgatggac tgtctgacgt gcgctggggc 1140cacgggcgcg atttcacgct tgaggagggt gaatacagga tcgcggaagg atcgctgcgg 1200catctgtacg atgcgatact tgcgttcaac gacccgctgt taaacgaccc gctccttcaa 1260gagatattcg atcttggtgc cgtggagagg aaactggcca atggcgacct ggaagcactc 1320ttggacaaat ggattgcgcg acccgagata gtgcttgacc ctagttga 136814455PRTArthrobacter species 14Val Ile Glu Phe Pro Glu Tyr Arg Asp Ser Ser Ala Ala Pro Lys Ile 1 5 10 15 Ser Asp Leu Glu Arg Leu Met Asp Arg Ser Leu Thr Asn Ser Gln Tyr 20 25 30 Val Asp Gly Ala Asn Ala Ala Arg Leu Leu Gly Thr Phe Ile Arg Ser 35 40 45 Phe Arg Ser Val Ile Gly Ser Ala Glu Glu Ser Ala Thr Arg Ala Asn 50 55 60 Leu Val Glu Ala His Asp Glu Ala Lys Leu Phe Gly Leu Met Leu Ser 65 70 75 80 Ala Gly Phe Asp Leu Ile Cys Asn Ala Glu Tyr Val His Gly Arg Leu 85 90 95 Val Asn Asn Lys Trp Ile Tyr Cys His Arg Gly Gly Glu Pro Ala Val 100 105 110 Ala Tyr Tyr Ser Phe Leu Lys Gln Cys Pro Arg Cys Cys Leu Asp Arg 115 120 125 Gly Leu Glu Gly Arg Leu Ser Gly Ala Gln His Lys Pro Thr Ser His 130 135 140 His Ile Gly Glu Ile Thr Thr Val Ala Ile Ala Leu Leu Leu Gln Leu 145 150 155 160 Val Ala Ala Ala Asn Glu Asn Pro Phe Glu Ile Ala Thr Ile Thr Lys 165 170 175 Gln Ser His Asp Val Asp Ala Ile Gly Phe Arg Asp Asp Leu Leu Val 180 185 190 Leu Phe Glu Ile Lys Ala Ser Pro Met Val Ser Phe Pro Leu Val Thr 195 200 205 Glu Leu Glu Glu Pro Met Leu Gln Glu Gly Pro Asp Gly Pro Val Glu 210 215 220 Tyr Arg Gln His Ser Leu Val Asp Leu Thr Leu Gln Gly Arg Glu Phe 225 230 235 240 Ala Val Ala Ile Pro His Ala Glu Thr Ala Ile Pro Leu Gly Glu Arg 245 250 255 Glu Gly Glu Ser Trp Pro Tyr Glu Pro Leu Ile Asp Tyr Phe Ser Val 260 265 270 Pro Ala Asn Ala Ala Ser Tyr Leu Gln Ala Trp Ile Glu Leu Tyr Ala 275 280 285 Ala Tyr Arg Thr Pro Lys Thr Gln Arg Ala Gly Arg Thr Ala Ala Leu 290 295 300 Ala Tyr Leu Val Asn Gly Trp Gly Asp Glu Ile Asp Ser Asn Lys Thr 305 310 315 320 Lys Pro Gly Leu Gly Arg Thr Asp Asp Val Lys Lys Gly Thr Tyr Gln 325 330 335 Leu Leu Lys Phe Gly Ser Tyr Tyr Arg Asp Asp Ala Ala Ser Val Pro 340 345 350 Val Arg Gly Ala Leu Val Ala Asn Leu Asp Pro Leu Phe Leu Arg Pro 355 360 365 Gly Tyr Ile Asp Gly Leu Ser Asp Val Arg Trp Gly His Gly Arg Asp 370 375 380 Phe Thr Leu Glu Glu Gly Glu Tyr Arg Ile Ala Glu Gly Ser Leu Arg 385 390 395 400 His Leu Tyr Asp Ala Ile Leu Ala Phe Asn Asp Pro Leu Leu Asn Asp 405 410 415 Pro Leu Leu Gln Glu Ile Phe Asp Leu Gly Ala Val Glu Arg Lys Leu 420 425 430 Ala Asn Gly Asp Leu Glu Ala Leu Leu Asp Lys Trp Ile Ala Arg Pro 435 440 445 Glu Ile Val Leu Asp Pro Ser 450 455 15735DNAAquaspirillum serpens 15atgttgagag gactaactgt ggaactttta gaattaaaaa atagaataac cacctcattt 60agcggtaccg aggatgattt aagagaagtt ctagacttga tagagcaaga tcagcctgtg 120tttccgttta atgagtatga gcatctcatt tgtaatctta tcgaaaaggg tggtcttaat 180tataatcaat atattgaaat tagatcggaa tatatcagtc aaaacccgaa cttatggatt 240tttgaaatat cagcccctag aggatttggt gagaaatttg cccaaacata tgtgaaaggt 300aagtgttcaa aactaaaaac cccatccaaa aaattagacc caaattatgc tggagaatat 360gatctttggc tcgacggaat tactattgag gtaaaagcat ctagagcagt agatagtaat 420agtgaagaac ctctttatgt gaaagcatta gcaagagata caaacaggca attcattatg 480aattttcagc aactgaaacc gcaatactgt gatgtattta tatgggttgc cgtctttcgg 540gatgaaattg tgttatggat aatgagttca gatgaggttg aaaaaaatcc tttttattca 600aaagggcaac atagaggcaa caaaggaaac gaagggcagt tgcatataaa acatgacaaa 660atccacttat tctcaaagta tgagcttaag gatgatgatt tggagggggc aatacggaaa 720gcagcaaaag gataa 73516244PRTAquaspirillum serpens 16Met Leu Arg Gly Leu Thr Val Glu Leu Leu Glu Leu Lys Asn Arg Ile 1 5 10 15 Thr Thr Ser Phe Ser Gly Thr Glu Asp Asp Leu Arg Glu Val Leu Asp 20 25 30 Leu Ile Glu Gln Asp Gln Pro Val Phe Pro Phe Asn Glu Tyr Glu His 35 40 45 Leu Ile Cys Asn Leu Ile Glu Lys Gly Gly Leu Asn Tyr Asn Gln Tyr 50 55 60 Ile Glu Ile Arg Ser Glu Tyr Ile Ser Gln Asn Pro Asn Leu Trp Ile 65 70 75 80 Phe Glu Ile Ser Ala Pro Arg Gly Phe Gly Glu Lys Phe Ala Gln Thr 85 90 95 Tyr Val Lys Gly Lys Cys Ser Lys Leu Lys Thr Pro Ser Lys Lys Leu 100 105 110 Asp Pro Asn Tyr Ala Gly Glu Tyr Asp Leu Trp Leu Asp Gly Ile Thr 115 120 125 Ile Glu Val Lys Ala Ser Arg Ala Val Asp Ser Asn Ser Glu Glu Pro 130 135 140 Leu Tyr Val Lys Ala Leu Ala Arg Asp Thr Asn Arg Gln Phe Ile Met 145 150 155 160 Asn Phe Gln Gln Leu Lys Pro Gln Tyr Cys Asp Val Phe Ile Trp Val 165 170 175 Ala Val Phe Arg Asp Glu Ile Val Leu Trp Ile Met Ser Ser Asp Glu 180 185 190 Val Glu Lys Asn Pro Phe Tyr Ser Lys Gly Gln His Arg Gly Asn Lys 195 200 205 Gly Asn Glu Gly Gln Leu His Ile Lys His Asp Lys Ile His Leu Phe 210 215 220 Ser Lys Tyr Glu Leu Lys Asp Asp Asp Leu Glu Gly Ala Ile Arg Lys 225 230 235 240 Ala Ala Lys Gly 17372DNAAcinetobacter species 1690 17aatttaaacg tgtattttgg taaaggtaga gaaaataaac aaactggaaa gatcattcct 60cgtccgtggt atgaaataga gatcatttct agtaacgata tcaatagttt acctgattat 120ccaaaaggtg acttctatgc atatacggat gatggcttaa ttattcctat gagaactcaa 180ggggactatt ttaaaaattt aagatcaaaa gatagcctgc aaatttttgg tatgtggctg 240aagggaaaac tagaaaaagc gggagtgtta aaaaaatata cgcctgttac gattgacact 300ttaagggagt atggtaatag caagctaaca ctttataaaa taagtgaaaa tgagtatttt 360atggattttt ag 37218334PRTAcinetobacter species 1690 18Met Phe Thr Asn Leu Asp Lys His His Gly Gly Asn Phe Arg Asp Val 1 5 10 15 Leu Glu Tyr Asn Leu Val Thr Ala Lys Lys Val Arg Ile Ala Ser Gly 20 25 30 Tyr Val Ser Leu His Thr Ile Gln Ala Tyr Arg Thr Gln Leu Glu Asp 35 40 45 Ile Ala Cys Arg Tyr Gly Asn Val Gln Leu Met Leu Gly Met Ala Phe 50 55 60 Tyr Glu Gly Leu Ser Val Lys Gln Leu Asp Ala Cys Leu Asp Leu His 65 70 75 80 Asn Ser Leu Ser Leu His Pro Asn Ser Gly Val Tyr Val Ala His Gly 85 90 95 Arg Arg Tyr His Gly Lys Ile Tyr Asp Phe Asn Glu Gly Val Asp Asn 100 105 110 Lys Ile Phe Val Gly Ser Ser Asn Phe Ser Pro Ser Gly Leu Ala Gly 115 120 125 Asn Ile Glu Cys Thr Val Glu Val Ile Asp Arg Ser Gln Lys Ala Gln 130 135 140 Val Asn Asn Phe Leu Asp Thr Leu Phe Asp Lys His Ser Glu Lys Ile 145 150 155 160 Asn Asn Val Val Ile Asn Thr Gly Thr Lys Arg Val Val Ser Leu Ser 165 170 175 Ile Asp Glu Lys Tyr Arg Lys Leu Leu Arg His Ser Arg Thr Ile Asn 180 185 190 Thr Ala Leu Asn Lys Val Glu Ile Asp Leu Glu Arg Ile Ala Glu Lys 195 200 205 Pro Ser Ser Asn Leu Asn Val Tyr Phe Gly Lys Gly Arg Glu Asn Lys 210 215 220 Gln Thr Gly Lys Ile Ile Pro Arg Pro Trp Tyr Glu Ile Glu Ile Ile 225 230 235 240 Ser Ser Asn Asp Ile Asn Ser Leu Pro Asp Tyr Pro Lys Gly Asp Phe 245 250 255 Tyr Ala Tyr Thr Asp Asp Gly Leu Ile Ile Pro Met Arg Thr Gln Gly 260 265 270 Asp Tyr Phe Lys Asn Leu Arg Ser Lys Asp Ser Leu Gln Ile Phe Gly 275 280 285 Met Trp Leu Lys Gly Lys Leu Glu Lys Ala Gly Val Leu Lys Lys Tyr 290 295 300 Thr Pro Val Thr Ile Asp Thr Leu Arg Glu Tyr Gly Asn Ser Lys Leu 305 310 315 320 Thr Leu Tyr Lys Ile Ser Glu Asn Glu Tyr Phe Met Asp Phe 325 330 191077DNAAnabaena variabilis uw 19atggaagaag accttgattt atctgaaaat atcgaagctg catctgcgga gcttacgact 60ctttatcagg tagctgctga tgctatgaaa gattatattg aaatctatct tgcgctgagt 120aaacagtctg atgggttttc aaatattaac aatcttgact taacttctcg taacaggcgt 180ttggtagtta tacatggact ttcgttagag ttagatccag atacttcgac tccagaggaa 240attaaacgtg aagctgaacg aatgctagcg atagctcttg atacagagtc agcaattacg 300gcaggagtat atgaaaaaat gcgtctcttc gcaagctctt tagtagatca gctatttgaa 360caaacggatg aacttaattc attatcatcg gaatatttgt cagcaaatcc aggatttttg 420ccgtttttcc agcagttggc ggggcttaga agtaaatcag agttaaagag agaagtagga 480aatgcctctg acaatagtat ttctaaagcg gttgcagaga gaatattaga gcgcattata 540cgtaacttga gaattcgcac tttttccaaa gagaaactat tacaagctgt tgagcctact 600ttagaaggaa tagtcaggga tctcgtagga aaagtgttat tggaaaatat agttgctgat 660gctttatctg atttacaagt tcctttcatg cgtgaatcag agtatcaaag ccttaaagga 720gtgatttatg atttccgcgc tgattttgtg ataccagacg cacaaaatcc aattgctttt 780atcgaggtgc gaaaaagctc tacacgacat gcgtcactct atgccaagga taagatgttt 840tcagcgatta attggaaagg aaaaaataaa aggcttttgg gtattttggt tgtggaagga 900ccttggacaa gagaaactct tcgcgtcatg gcaaatgtgt ttgattacgt tacaccttta 960actcgtgttt cccaagttgc agaagctatc agagcatatc tagatgggga taaaacgaga 1020ctgaagtggt tagttaattt cagtattgaa gaagcagacc acgacaacat aacctaa

107720358PRTAnabaena variabilis uw 20Met Glu Glu Asp Leu Asp Leu Ser Glu Asn Ile Glu Ala Ala Ser Ala 1 5 10 15 Glu Leu Thr Thr Leu Tyr Gln Val Ala Ala Asp Ala Met Lys Asp Tyr 20 25 30 Ile Glu Ile Tyr Leu Ala Leu Ser Lys Gln Ser Asp Gly Phe Ser Asn 35 40 45 Ile Asn Asn Leu Asp Leu Thr Ser Arg Asn Arg Arg Leu Val Val Ile 50 55 60 His Gly Leu Ser Leu Glu Leu Asp Pro Asp Thr Ser Thr Pro Glu Glu 65 70 75 80 Ile Lys Arg Glu Ala Glu Arg Met Leu Ala Ile Ala Leu Asp Thr Glu 85 90 95 Ser Ala Ile Thr Ala Gly Val Tyr Glu Lys Met Arg Leu Phe Ala Ser 100 105 110 Ser Leu Val Asp Gln Leu Phe Glu Gln Thr Asp Glu Leu Asn Ser Leu 115 120 125 Ser Ser Glu Tyr Leu Ser Ala Asn Pro Gly Phe Leu Pro Phe Phe Gln 130 135 140 Gln Leu Ala Gly Leu Arg Ser Lys Ser Glu Leu Lys Arg Glu Val Gly 145 150 155 160 Asn Ala Ser Asp Asn Ser Ile Ser Lys Ala Val Ala Glu Arg Ile Leu 165 170 175 Glu Arg Ile Ile Arg Asn Leu Arg Ile Arg Thr Phe Ser Lys Glu Lys 180 185 190 Leu Leu Gln Ala Val Glu Pro Thr Leu Glu Gly Ile Val Arg Asp Leu 195 200 205 Val Gly Lys Val Leu Leu Glu Asn Ile Val Ala Asp Ala Leu Ser Asp 210 215 220 Leu Gln Val Pro Phe Met Arg Glu Ser Glu Tyr Gln Ser Leu Lys Gly 225 230 235 240 Val Ile Tyr Asp Phe Arg Ala Asp Phe Val Ile Pro Asp Ala Gln Asn 245 250 255 Pro Ile Ala Phe Ile Glu Val Arg Lys Ser Ser Thr Arg His Ala Ser 260 265 270 Leu Tyr Ala Lys Asp Lys Met Phe Ser Ala Ile Asn Trp Lys Gly Lys 275 280 285 Asn Lys Arg Leu Leu Gly Ile Leu Val Val Glu Gly Pro Trp Thr Arg 290 295 300 Glu Thr Leu Arg Val Met Ala Asn Val Phe Asp Tyr Val Thr Pro Leu 305 310 315 320 Thr Arg Val Ser Gln Val Ala Glu Ala Ile Arg Ala Tyr Leu Asp Gly 325 330 335 Asp Lys Thr Arg Leu Lys Trp Leu Val Asn Phe Ser Ile Glu Glu Ala 340 345 350 Asp His Asp Asn Ile Thr 355 211620DNABacillus brevismisc_feature(64)..(64)r= g or a 21atgacagaaa gaaaagtctg gtttattaca agaccggagc gtgatccaaa gtttcacagg 60gaarcccttc tggctctgca gaaagcaaca aacggcttca cagtaaagtg gtccgkaaac 120cgcacagcac atctcgcata tgagcaggcg ctggccgatg ctgaggtaaa gcgtccaaac 180atcagtaatg atggctctgg tggacgaaca tgggccgcaa tgctgaagac atttgcctac 240tgctatacaa acgaagaggg ctaccttgta ccgaccaaag tcggagaagc actgttaaaa 300aggcacaaag tattcgacaa cgttaaaaaa cagattctca ctcttcagat tccgaatgcc 360tattttctgg aggccggatt ccggccaaaa tttgatgaat ctttccgtat tcgtccagcc 420cggttcctga tccgcctagt aaatcaagag gagctggcct accacgtcac gaaagaagaa 480attactttct ttgcgctaac agcatcaaag gacagccagt tgtcagagat tacagcaaaa 540atcaaagcat ttcgcgttgc ttcttctgct gaaagactag aaatgaaagc agacatcgct 600gctcaatatg accatcgtga gcgaacagat aaaggggcgc gcgattttga gactgctcac 660tctgatgttg cccatacctt tatgctaatc tgcgacgcca caggaatggt tgagtacatt 720cgcggtcaat ccctgagggt aaatccagaa gaaaatcaga agcttagcca ggagttggag 780gagctggagg cacggtatcc cttcaacaac cgatacaaaa tttccctaga acggatggca 840gaaaacaatg gtcttgatgt cgaaagctac aaggcgagcc gtaatagcgg aaaaggacaa 900gcgacaaatg cagcaaagag actgagaaaa ataaacgaaa tcatgaacgc gtatcccaat 960cccgctgctt tgccgcagga agaactggag agaatcctcg cagaagaggt cggtccgcgt 1020gaagctcaaa agtatgcatt cgaattaaaa gaaagtcagg tagccttcag cggactgaat 1080acagagttcg tagagagtta tctgtatgaa gaagacaatc tccgattcga agacaaaaca 1140ggggaagtgc tcaaagcgat cggttttgac gttgaaatgc ggcccaaacc tgcatccatg 1200gagcgaacag aaattgagat catggtgaag tatggcgata ggcagtgcgg tattattgat 1260gccaagaact accggcaaaa gtttgctctt tctgcctcac tgacatcgca tatggcatcc 1320gagtatatac cgaactatca gggatacaag gggcttaatg tacagttttt tggatatgta 1380accgctgctg acttttctgg cgaaaaaaat cttgaaaaaa tcagcaataa agtacaggaa 1440cacacttcta gcagagacat aaaaggacta atgctcagcg ctaaagtatt gcttggattt 1500cttgattact gcttagagaa cgatattccc gaaaacgaac gtgtgaatct gtttatacgc 1560gctgtccaaa accggggcta caaaacgctg ggagagatgc tgaaagaagc taaatactaa 162022539PRTBacillus brevismisc_feature(22)..(22)Xaa can be any naturally occurring amino acid 22Met Thr Glu Arg Lys Val Trp Phe Ile Thr Arg Pro Glu Arg Asp Pro 1 5 10 15 Lys Phe His Arg Glu Xaa Leu Leu Ala Leu Gln Lys Ala Thr Asn Gly 20 25 30 Phe Thr Val Lys Trp Ser Xaa Asn Arg Thr Ala His Leu Ala Tyr Glu 35 40 45 Gln Ala Leu Ala Asp Ala Glu Val Lys Arg Pro Asn Ile Ser Asn Asp 50 55 60 Gly Ser Gly Gly Arg Thr Trp Ala Ala Met Leu Lys Thr Phe Ala Tyr 65 70 75 80 Cys Tyr Thr Asn Glu Glu Gly Tyr Leu Val Pro Thr Lys Val Gly Glu 85 90 95 Ala Leu Leu Lys Arg His Lys Val Phe Asp Asn Val Lys Lys Gln Ile 100 105 110 Leu Thr Leu Gln Ile Pro Asn Ala Tyr Phe Leu Glu Ala Gly Phe Arg 115 120 125 Pro Lys Phe Asp Glu Ser Phe Arg Ile Arg Pro Ala Arg Phe Leu Ile 130 135 140 Arg Leu Val Asn Gln Glu Glu Leu Ala Tyr His Val Thr Lys Glu Glu 145 150 155 160 Ile Thr Phe Phe Ala Leu Thr Ala Ser Lys Asp Ser Gln Leu Ser Glu 165 170 175 Ile Thr Ala Lys Ile Lys Ala Phe Arg Val Ala Ser Ser Ala Glu Arg 180 185 190 Leu Glu Met Lys Ala Asp Ile Ala Ala Gln Tyr Asp His Arg Glu Arg 195 200 205 Thr Asp Lys Gly Ala Arg Asp Phe Glu Thr Ala His Ser Asp Val Ala 210 215 220 His Thr Phe Met Leu Ile Cys Asp Ala Thr Gly Met Val Glu Tyr Ile 225 230 235 240 Arg Gly Gln Ser Leu Arg Val Asn Pro Glu Glu Asn Gln Lys Leu Ser 245 250 255 Gln Glu Leu Glu Glu Leu Glu Ala Arg Tyr Pro Phe Asn Asn Arg Tyr 260 265 270 Lys Ile Ser Leu Glu Arg Met Ala Glu Asn Asn Gly Leu Asp Val Glu 275 280 285 Ser Tyr Lys Ala Ser Arg Asn Ser Gly Lys Gly Gln Ala Thr Asn Ala 290 295 300 Ala Lys Arg Leu Arg Lys Ile Asn Glu Ile Met Asn Ala Tyr Pro Asn 305 310 315 320 Pro Ala Ala Leu Pro Gln Glu Glu Leu Glu Arg Ile Leu Ala Glu Glu 325 330 335 Val Gly Pro Arg Glu Ala Gln Lys Tyr Ala Phe Glu Leu Lys Glu Ser 340 345 350 Gln Val Ala Phe Ser Gly Leu Asn Thr Glu Phe Val Glu Ser Tyr Leu 355 360 365 Tyr Glu Glu Asp Asn Leu Arg Phe Glu Asp Lys Thr Gly Glu Val Leu 370 375 380 Lys Ala Ile Gly Phe Asp Val Glu Met Arg Pro Lys Pro Ala Ser Met 385 390 395 400 Glu Arg Thr Glu Ile Glu Ile Met Val Lys Tyr Gly Asp Arg Gln Cys 405 410 415 Gly Ile Ile Asp Ala Lys Asn Tyr Arg Gln Lys Phe Ala Leu Ser Ala 420 425 430 Ser Leu Thr Ser His Met Ala Ser Glu Tyr Ile Pro Asn Tyr Gln Gly 435 440 445 Tyr Lys Gly Leu Asn Val Gln Phe Phe Gly Tyr Val Thr Ala Ala Asp 450 455 460 Phe Ser Gly Glu Lys Asn Leu Glu Lys Ile Ser Asn Lys Val Gln Glu 465 470 475 480 His Thr Ser Ser Arg Asp Ile Lys Gly Leu Met Leu Ser Ala Lys Val 485 490 495 Leu Leu Gly Phe Leu Asp Tyr Cys Leu Glu Asn Asp Ile Pro Glu Asn 500 505 510 Glu Arg Val Asn Leu Phe Ile Arg Ala Val Gln Asn Arg Gly Tyr Lys 515 520 525 Thr Leu Gly Glu Met Leu Lys Glu Ala Lys Tyr 530 535 23840DNABacillus brevis 23atgggggtag taatgattaa cgaggacttt tttatttatg agcaattgtc tcacaagaaa 60aatttagagc aaaaggggaa aaatgcattt gatgaagaga cggaggaact tgtaaggcaa 120gccaaaagtg gctatcatgc ctttattgaa ggaataaatt atgacgaagt aacaaaactg 180gatctcaata gttctgtagc tgcattagaa gattacatct ccattgcgaa agaaatagag 240aaaaaacata aaatgtttaa ctggcgaagt gactatgctg gaagcattat tccagaattt 300ttgtatagaa ttgtgcatgt agcaactgtg aaagctgggt taaaacctat tttctctacg 360agaaatacaa ttattgagat cagtggagcg gcacataggg aaggattaca aatacgacgt 420aaaaacgaag attttgcgtt gggttttcat gaggtagacg ttaaaattgc aagtgagagt 480catagagtta ttagtttagc cgtcgcatgt gaagttaaaa caaatatcga taaaaacaaa 540cttaatgggt tagacttttc ggctgagcgg atgaaacgca catatccagg ttctgcttat 600tttttaataa ccgagaccct agatttttcc ccagatgaga atcattcatc tggtctcatc 660gatgaaattt atgttcttcg aaaacaagtg cgcaccaaaa accgagttca gaaggcaccg 720ctatgcccta gtgtttttgc agagttgttg gaagacattc ttgaaatatc ataccgtgca 780tctaatgtaa aaggacatgt ttatgatcgt ttggagggag ggaagttaat acgtgtttaa 84024279PRTBacillus brevis 24Met Gly Val Val Met Ile Asn Glu Asp Phe Phe Ile Tyr Glu Gln Leu 1 5 10 15 Ser His Lys Lys Asn Leu Glu Gln Lys Gly Lys Asn Ala Phe Asp Glu 20 25 30 Glu Thr Glu Glu Leu Val Arg Gln Ala Lys Ser Gly Tyr His Ala Phe 35 40 45 Ile Glu Gly Ile Asn Tyr Asp Glu Val Thr Lys Leu Asp Leu Asn Ser 50 55 60 Ser Val Ala Ala Leu Glu Asp Tyr Ile Ser Ile Ala Lys Glu Ile Glu 65 70 75 80 Lys Lys His Lys Met Phe Asn Trp Arg Ser Asp Tyr Ala Gly Ser Ile 85 90 95 Ile Pro Glu Phe Leu Tyr Arg Ile Val His Val Ala Thr Val Lys Ala 100 105 110 Gly Leu Lys Pro Ile Phe Ser Thr Arg Asn Thr Ile Ile Glu Ile Ser 115 120 125 Gly Ala Ala His Arg Glu Gly Leu Gln Ile Arg Arg Lys Asn Glu Asp 130 135 140 Phe Ala Leu Gly Phe His Glu Val Asp Val Lys Ile Ala Ser Glu Ser 145 150 155 160 His Arg Val Ile Ser Leu Ala Val Ala Cys Glu Val Lys Thr Asn Ile 165 170 175 Asp Lys Asn Lys Leu Asn Gly Leu Asp Phe Ser Ala Glu Arg Met Lys 180 185 190 Arg Thr Tyr Pro Gly Ser Ala Tyr Phe Leu Ile Thr Glu Thr Leu Asp 195 200 205 Phe Ser Pro Asp Glu Asn His Ser Ser Gly Leu Ile Asp Glu Ile Tyr 210 215 220 Val Leu Arg Lys Gln Val Arg Thr Lys Asn Arg Val Gln Lys Ala Pro 225 230 235 240 Leu Cys Pro Ser Val Phe Ala Glu Leu Leu Glu Asp Ile Leu Glu Ile 245 250 255 Ser Tyr Arg Ala Ser Asn Val Lys Gly His Val Tyr Asp Arg Leu Glu 260 265 270 Gly Gly Lys Leu Ile Arg Val 275 251608DNABacteroides caccae 25atgcctagaa aacctgaata taagccgttg ctttacacga ctacgatacg aaatcctgag 60cgttttaaag atttcatgca catacttaaa cgattcaatg gccggatact taataataaa 120acagtcgagt tgttcgagag agaactgttt aaggttggct tgtatcgacc aatgaagcgc 180ccggaaacag ttcaggataa gtggaaatca acaaagaacg gggaattagc cagcaaacca 240ttaacagatg aagaaacgaa agatgtgtat cagcagaatg atccccaagt caacaaaagt 300ataaagggac ataaagaagc agggtttcct aaagggtggc cgagtcgatt tgacacacaa 360ttcaaattga tgaaagttct gggctttgta tattatgaat ggggaaagcc tataaacttc 420tctcaaacag gtaactatct tgcagatact gtatccattg aaatagattc aggagcaata 480tctcgcgaga ttgtaaatcc acagaatgag caaattgcat ttatgcaagc ttttgccaag 540caacaaagat gcaatccgtt tatttgtgaa ttaaatgata atattccact gatattattg 600cttgaagtta taaagaagtt aaactctgat ccagattata atggttcggg aatctcatat 660aaggagatac ctttagttat cttttggaaa gataatgatg cggaatcttt gtatcagcgt 720attaaacttc tgcgaaagga acataggtac aatccttcaa atgaagtgat agaggatata 780tgtgtaaacg aaatacttgg gggattcaag aaatttgatc ttgactctat tgtgtccgaa 840tatcccgatg aatttgtccg caaaatgaga atgacaggac ttatatcatt tagagggggt 900ggtcgattta tcgacattaa ccataatgaa gatgataaga taaattatat actggctaat 960tatgccacat atcgcaagta cacttcaaaa gaagaatatt ttgactatat gtcagacatt 1020gatgatgcat tgtttgcatt aaaagctgtc gaaatcccca aaaatgtcgc agctgataaa 1080ttagctaaac tcgtgggtga ttactcatgg gattctatta aaaaagaact tacccatttg 1140gcgaaaaaaa catcatcaag ccacaatatt ttaaggttta ttgcagctcc ggcaagatta 1200gagttcctga ccgcccttgc tattaagtca aaactgcctg ctgttgaggt aattcccaat 1260tatccatgtg acgatgaagg tctgccaacc tctacggctg gtggggatat tggagatatt 1320gagtgtttcg aggcttctaa cagtatattg gtggaagtca caatgtctga agggcgtcag 1380cagacaatga tggaagtatg gcctattgcg agacatttaa aggagcttag agaaaaatat 1440gaatgtgaaa atttccaatg tgtgtttctg gcaccaagta tatttgttga ttctgagaat 1500cagatagact gggttaagga taaaaagcag cttgttattc gtccatacaa gattgtagat 1560tttattaact atctggatac agcagcatct ttatatcaga ttgtataa 160826535PRTBacteroides caccae 26Met Pro Arg Lys Pro Glu Tyr Lys Pro Leu Leu Tyr Thr Thr Thr Ile 1 5 10 15 Arg Asn Pro Glu Arg Phe Lys Asp Phe Met His Ile Leu Lys Arg Phe 20 25 30 Asn Gly Arg Ile Leu Asn Asn Lys Thr Val Glu Leu Phe Glu Arg Glu 35 40 45 Leu Phe Lys Val Gly Leu Tyr Arg Pro Met Lys Arg Pro Glu Thr Val 50 55 60 Gln Asp Lys Trp Lys Ser Thr Lys Asn Gly Glu Leu Ala Ser Lys Pro 65 70 75 80 Leu Thr Asp Glu Glu Thr Lys Asp Val Tyr Gln Gln Asn Asp Pro Gln 85 90 95 Val Asn Lys Ser Ile Lys Gly His Lys Glu Ala Gly Phe Pro Lys Gly 100 105 110 Trp Pro Ser Arg Phe Asp Thr Gln Phe Lys Leu Met Lys Val Leu Gly 115 120 125 Phe Val Tyr Tyr Glu Trp Gly Lys Pro Ile Asn Phe Ser Gln Thr Gly 130 135 140 Asn Tyr Leu Ala Asp Thr Val Ser Ile Glu Ile Asp Ser Gly Ala Ile 145 150 155 160 Ser Arg Glu Ile Val Asn Pro Gln Asn Glu Gln Ile Ala Phe Met Gln 165 170 175 Ala Phe Ala Lys Gln Gln Arg Cys Asn Pro Phe Ile Cys Glu Leu Asn 180 185 190 Asp Asn Ile Pro Leu Ile Leu Leu Leu Glu Val Ile Lys Lys Leu Asn 195 200 205 Ser Asp Pro Asp Tyr Asn Gly Ser Gly Ile Ser Tyr Lys Glu Ile Pro 210 215 220 Leu Val Ile Phe Trp Lys Asp Asn Asp Ala Glu Ser Leu Tyr Gln Arg 225 230 235 240 Ile Lys Leu Leu Arg Lys Glu His Arg Tyr Asn Pro Ser Asn Glu Val 245 250 255 Ile Glu Asp Ile Cys Val Asn Glu Ile Leu Gly Gly Phe Lys Lys Phe 260 265 270 Asp Leu Asp Ser Ile Val Ser Glu Tyr Pro Asp Glu Phe Val Arg Lys 275 280 285 Met Arg Met Thr Gly Leu Ile Ser Phe Arg Gly Gly Gly Arg Phe Ile 290 295 300 Asp Ile Asn His Asn Glu Asp Asp Lys Ile Asn Tyr Ile Leu Ala Asn 305 310 315 320 Tyr Ala Thr Tyr Arg Lys Tyr Thr Ser Lys Glu Glu Tyr Phe Asp Tyr 325 330 335 Met Ser Asp Ile Asp Asp Ala Leu Phe Ala Leu Lys Ala Val Glu Ile 340 345 350 Pro Lys Asn Val Ala Ala Asp Lys Leu Ala Lys Leu Val Gly Asp Tyr 355 360 365 Ser Trp Asp Ser Ile Lys Lys Glu Leu Thr His Leu Ala Lys Lys Thr 370 375 380 Ser Ser Ser His Asn Ile Leu Arg Phe Ile Ala Ala Pro Ala Arg Leu 385 390 395 400 Glu Phe Leu Thr Ala Leu Ala Ile Lys Ser Lys Leu Pro Ala Val Glu 405 410 415 Val Ile Pro Asn Tyr Pro Cys Asp Asp Glu Gly Leu Pro Thr Ser Thr 420 425 430 Ala Gly Gly Asp Ile Gly Asp Ile Glu Cys Phe Glu Ala Ser Asn Ser 435 440 445 Ile Leu Val Glu Val Thr Met Ser Glu Gly Arg Gln Gln Thr Met Met 450 455 460

Glu Val Trp Pro Ile Ala Arg His Leu Lys Glu Leu Arg Glu Lys Tyr 465 470 475 480 Glu Cys Glu Asn Phe Gln Cys Val Phe Leu Ala Pro Ser Ile Phe Val 485 490 495 Asp Ser Glu Asn Gln Ile Asp Trp Val Lys Asp Lys Lys Gln Leu Val 500 505 510 Ile Arg Pro Tyr Lys Ile Val Asp Phe Ile Asn Tyr Leu Asp Thr Ala 515 520 525 Ala Ser Leu Tyr Gln Ile Val 530 535 271776DNABacillus cereus 1315 27atggtacaaa aaaatagaag taaagaggta tggcttgttc caaaaagagg aagttttcac 60caaacgattt gtttaataga atcccttata aataggaatt atgatcaaac acgttggaat 120gagcaaaaac aaaataatat tggaaatgat ttaagaaaac gtggggcagt aagggaaaaa 180agatcccctt caaatcaatc tattcgtact ttacttgctt caattccaca gtatttaggg 240tttttataca tagatagcaa tacaacgcct aatactgtaa aaatcacaga tgctggtaga 300tacctatata attttcataa agatagcatt gagaatatcg gaaccttagg ggaaggtaaa 360aaaagtggag gtttaattga aacttcatcg gtatttcttg aacaatttga aaaacttcaa 420atcactaatc cggtaatatt aaaagattgt gaaaatattt tagtctttcc atttagagtt 480attttaaagt tattaattga attaaattat cttgatcgag aggaattggc gtattttgta 540ttttcgatta gggatgaaag tgaaattcca cttacaatag agaaaataaa aaagtacagg 600aaacaagatt taatggaacg agatactgaa attaaacttt ttaaggaaac acatataggt 660aatattactc ttgtaaaggc atcttcagca tcttattttg aaaacctgtg ttatagtacg 720gggattattg agagatttaa aattcagata ccgaaccctg gaagctctga ttccaataaa 780ttacctgcaa ttaaaattaa agatgaacaa gaagtttatg ttaaagaggt tttaagtagt 840aaatatgaaa attcacaagt atataatttt ggtaataatt taaagttatg ggtggattac 900attggaaatc ctaatagaaa gataccacca agggatattg agattgaaaa taaaggaaat 960agcaatttaa taataattat tgaacaaaat ggggtaatga taaaaggtga tttaataaaa 1020agtggttact cattagtttc tccaatgttc ataaatgaga attatgacat tatttttata 1080agcccggtgg atggaactgt tttggaaagg gctacaatca agccggatta tttaagtgga 1140aaatatgaat ttaatattaa ctcaaacctt agtattacta ataatgaaaa tatagatgaa 1200ataggacaaa tcatcaatga acactctgcg gcaaaaacat ttgataagaa ttatttgtct 1260tatctaggaa ttataggaga cattatagga gctgatttaa ccaataataa aaaccttagg 1320ggagcttatt atgaatattt attttataaa ttattggagc agctacgaaa agaaaaaata 1380atagatgatg tgtattggaa tggtaaagta ggggaatttg gtcttccaag accagcgcct 1440ggggggaaga caggaacccc agattttatt tttattatta atgatgaatt ctttatatta 1500gaattaccaa caattaaggc taattctgca caatttagtg ctgaaggttc ttcattacca 1560gaccacatta atttatttgc ggaagaacct tctgaggcaa ttgtctatgg aatttatact 1620gcacccacta ttcatgatcg gaatacatct gccatgaaag cgattcttga tccactagaa 1680attaatttaa aatgtattga agatagagaa ttagtagatt tgttattatc taaagataga 1740aatctcattt acagtgaatt aacaagtggt aaataa 177628591PRTBacillus cereus 1315 28Met Val Gln Lys Asn Arg Ser Lys Glu Val Trp Leu Val Pro Lys Arg 1 5 10 15 Gly Ser Phe His Gln Thr Ile Cys Leu Ile Glu Ser Leu Ile Asn Arg 20 25 30 Asn Tyr Asp Gln Thr Arg Trp Asn Glu Gln Lys Gln Asn Asn Ile Gly 35 40 45 Asn Asp Leu Arg Lys Arg Gly Ala Val Arg Glu Lys Arg Ser Pro Ser 50 55 60 Asn Gln Ser Ile Arg Thr Leu Leu Ala Ser Ile Pro Gln Tyr Leu Gly 65 70 75 80 Phe Leu Tyr Ile Asp Ser Asn Thr Thr Pro Asn Thr Val Lys Ile Thr 85 90 95 Asp Ala Gly Arg Tyr Leu Tyr Asn Phe His Lys Asp Ser Ile Glu Asn 100 105 110 Ile Gly Thr Leu Gly Glu Gly Lys Lys Ser Gly Gly Leu Ile Glu Thr 115 120 125 Ser Ser Val Phe Leu Glu Gln Phe Glu Lys Leu Gln Ile Thr Asn Pro 130 135 140 Val Ile Leu Lys Asp Cys Glu Asn Ile Leu Val Phe Pro Phe Arg Val 145 150 155 160 Ile Leu Lys Leu Leu Ile Glu Leu Asn Tyr Leu Asp Arg Glu Glu Leu 165 170 175 Ala Tyr Phe Val Phe Ser Ile Arg Asp Glu Ser Glu Ile Pro Leu Thr 180 185 190 Ile Glu Lys Ile Lys Lys Tyr Arg Lys Gln Asp Leu Met Glu Arg Asp 195 200 205 Thr Glu Ile Lys Leu Phe Lys Glu Thr His Ile Gly Asn Ile Thr Leu 210 215 220 Val Lys Ala Ser Ser Ala Ser Tyr Phe Glu Asn Leu Cys Tyr Ser Thr 225 230 235 240 Gly Ile Ile Glu Arg Phe Lys Ile Gln Ile Pro Asn Pro Gly Ser Ser 245 250 255 Asp Ser Asn Lys Leu Pro Ala Ile Lys Ile Lys Asp Glu Gln Glu Val 260 265 270 Tyr Val Lys Glu Val Leu Ser Ser Lys Tyr Glu Asn Ser Gln Val Tyr 275 280 285 Asn Phe Gly Asn Asn Leu Lys Leu Trp Val Asp Tyr Ile Gly Asn Pro 290 295 300 Asn Arg Lys Ile Pro Pro Arg Asp Ile Glu Ile Glu Asn Lys Gly Asn 305 310 315 320 Ser Asn Leu Ile Ile Ile Ile Glu Gln Asn Gly Val Met Ile Lys Gly 325 330 335 Asp Leu Ile Lys Ser Gly Tyr Ser Leu Val Ser Pro Met Phe Ile Asn 340 345 350 Glu Asn Tyr Asp Ile Ile Phe Ile Ser Pro Val Asp Gly Thr Val Leu 355 360 365 Glu Arg Ala Thr Ile Lys Pro Asp Tyr Leu Ser Gly Lys Tyr Glu Phe 370 375 380 Asn Ile Asn Ser Asn Leu Ser Ile Thr Asn Asn Glu Asn Ile Asp Glu 385 390 395 400 Ile Gly Gln Ile Ile Asn Glu His Ser Ala Ala Lys Thr Phe Asp Lys 405 410 415 Asn Tyr Leu Ser Tyr Leu Gly Ile Ile Gly Asp Ile Ile Gly Ala Asp 420 425 430 Leu Thr Asn Asn Lys Asn Leu Arg Gly Ala Tyr Tyr Glu Tyr Leu Phe 435 440 445 Tyr Lys Leu Leu Glu Gln Leu Arg Lys Glu Lys Ile Ile Asp Asp Val 450 455 460 Tyr Trp Asn Gly Lys Val Gly Glu Phe Gly Leu Pro Arg Pro Ala Pro 465 470 475 480 Gly Gly Lys Thr Gly Thr Pro Asp Phe Ile Phe Ile Ile Asn Asp Glu 485 490 495 Phe Phe Ile Leu Glu Leu Pro Thr Ile Lys Ala Asn Ser Ala Gln Phe 500 505 510 Ser Ala Glu Gly Ser Ser Leu Pro Asp His Ile Asn Leu Phe Ala Glu 515 520 525 Glu Pro Ser Glu Ala Ile Val Tyr Gly Ile Tyr Thr Ala Pro Thr Ile 530 535 540 His Asp Arg Asn Thr Ser Ala Met Lys Ala Ile Leu Asp Pro Leu Glu 545 550 555 560 Ile Asn Leu Lys Cys Ile Glu Asp Arg Glu Leu Val Asp Leu Leu Leu 565 570 575 Ser Lys Asp Arg Asn Leu Ile Tyr Ser Glu Leu Thr Ser Gly Lys 580 585 590 29858DNABacillus caldolyticus 29atgcaaccaa atcctaaatt tataaataaa agctctgcat tttgggctta tgcaaaactg 60ttgtctgaac agttaggata ttctaaagat ggagtagtca ttagttattc agaggcacag 120gcaagagcaa aacttaaaaa actaggtata aatgtaaaag agggtatttt taaagatgta 180ttgaggtacc tgaaatacag agcagaatta ctaaataaac ataaggacta tctaatggat 240gtagaagaag caaggaaata tttccaagta gcacttaagc aacatcagca gaataattat 300acttgcaaac ttccgcttaa caaacagaaa aatgaaaaga aagattatgc ttactttaca 360tgcattatta atattattgc agaaacggag ctaaggtatt ttgcaaacaa taatggttta 420gtttatggaa aagacattta ttttgatgat aatcctatga atctatcata tatattaaat 480ttcaatagag aattggaagg tataatgtcc cggcgttttg atggtgcttt tccaagtaca 540gtaaatccga ttctaatttg ggaaattaaa gagtattatt acacaaccac ttttggaagt 600cgaattgccg atggggttta tgaaactcag ttagatggct acgaaataaa aacaatcagg 660gaagaaacaa acaagaatat tcaacatata tactttattg atgactataa tacttggtgg 720aacatgggta agtcttatct ttgtcggatc attgatatgt tacatatggg attagtggac 780gaggtaatta tggggaaaga ggttttcgaa agatggcctc agattttaag agcagtactt 840aatcaatact ataaataa 85830285PRTBacillus caldolyticus 30Met Gln Pro Asn Pro Lys Phe Ile Asn Lys Ser Ser Ala Phe Trp Ala 1 5 10 15 Tyr Ala Lys Leu Leu Ser Glu Gln Leu Gly Tyr Ser Lys Asp Gly Val 20 25 30 Val Ile Ser Tyr Ser Glu Ala Gln Ala Arg Ala Lys Leu Lys Lys Leu 35 40 45 Gly Ile Asn Val Lys Glu Gly Ile Phe Lys Asp Val Leu Arg Tyr Leu 50 55 60 Lys Tyr Arg Ala Glu Leu Leu Asn Lys His Lys Asp Tyr Leu Met Asp 65 70 75 80 Val Glu Glu Ala Arg Lys Tyr Phe Gln Val Ala Leu Lys Gln His Gln 85 90 95 Gln Asn Asn Tyr Thr Cys Lys Leu Pro Leu Asn Lys Gln Lys Asn Glu 100 105 110 Lys Lys Asp Tyr Ala Tyr Phe Thr Cys Ile Ile Asn Ile Ile Ala Glu 115 120 125 Thr Glu Leu Arg Tyr Phe Ala Asn Asn Asn Gly Leu Val Tyr Gly Lys 130 135 140 Asp Ile Tyr Phe Asp Asp Asn Pro Met Asn Leu Ser Tyr Ile Leu Asn 145 150 155 160 Phe Asn Arg Glu Leu Glu Gly Ile Met Ser Arg Arg Phe Asp Gly Ala 165 170 175 Phe Pro Ser Thr Val Asn Pro Ile Leu Ile Trp Glu Ile Lys Glu Tyr 180 185 190 Tyr Tyr Thr Thr Thr Phe Gly Ser Arg Ile Ala Asp Gly Val Tyr Glu 195 200 205 Thr Gln Leu Asp Gly Tyr Glu Ile Lys Thr Ile Arg Glu Glu Thr Asn 210 215 220 Lys Asn Ile Gln His Ile Tyr Phe Ile Asp Asp Tyr Asn Thr Trp Trp 225 230 235 240 Asn Met Gly Lys Ser Tyr Leu Cys Arg Ile Ile Asp Met Leu His Met 245 250 255 Gly Leu Val Asp Glu Val Ile Met Gly Lys Glu Val Phe Glu Arg Trp 260 265 270 Pro Gln Ile Leu Arg Ala Val Leu Asn Gln Tyr Tyr Lys 275 280 285 31618DNABacteroides fragilis 31atggcaaagt caaagataaa atttaatgat gtgtcttctg ctaatggtac tcagaaaata 60cagcttccaa aatactcttc tcaagttatt aaccttgcaa atggttattc aaaggcaacc 120agaccggcaa acgttggaca ggtatctgaa gatataaaaa ctttcagaga tgatgagact 180cttataggat atacaaacca agattggata aactggcata aaaataaata tccagagggc 240atacaaaagg ctactgatgc aacatgggtt atgttccaaa agatggtaca aagtctcaat 300actgtaacta aagaagatat tcaaaagtgg gaagaggatt ttgtattttc gaaaacctat 360gatggattaa tggtccaaaa tgccatcgtt aagaaaatag cagaagagat aaacactcaa 420aactatcggt tagcttcacc cgaggaagaa cgacaaggta ttgatggcta cataaataat 480catccagtcc aaattaagtc agatacatat gatagaacgg gaagacttca taacgaagaa 540atgcaatgtg ttgtaatatc ataccaaaaa agcaataaga ctataatatt tgactacaat 600ccagaagatt ttcaataa 61832205PRTBacteroides fragilis 32Met Ala Lys Ser Lys Ile Lys Phe Asn Asp Val Ser Ser Ala Asn Gly 1 5 10 15 Thr Gln Lys Ile Gln Leu Pro Lys Tyr Ser Ser Gln Val Ile Asn Leu 20 25 30 Ala Asn Gly Tyr Ser Lys Ala Thr Arg Pro Ala Asn Val Gly Gln Val 35 40 45 Ser Glu Asp Ile Lys Thr Phe Arg Asp Asp Glu Thr Leu Ile Gly Tyr 50 55 60 Thr Asn Gln Asp Trp Ile Asn Trp His Lys Asn Lys Tyr Pro Glu Gly 65 70 75 80 Ile Gln Lys Ala Thr Asp Ala Thr Trp Val Met Phe Gln Lys Met Val 85 90 95 Gln Ser Leu Asn Thr Val Thr Lys Glu Asp Ile Gln Lys Trp Glu Glu 100 105 110 Asp Phe Val Phe Ser Lys Thr Tyr Asp Gly Leu Met Val Gln Asn Ala 115 120 125 Ile Val Lys Lys Ile Ala Glu Glu Ile Asn Thr Gln Asn Tyr Arg Leu 130 135 140 Ala Ser Pro Glu Glu Glu Arg Gln Gly Ile Asp Gly Tyr Ile Asn Asn 145 150 155 160 His Pro Val Gln Ile Lys Ser Asp Thr Tyr Asp Arg Thr Gly Arg Leu 165 170 175 His Asn Glu Glu Met Gln Cys Val Val Ile Ser Tyr Gln Lys Ser Asn 180 185 190 Lys Thr Ile Ile Phe Asp Tyr Asn Pro Glu Asp Phe Gln 195 200 205 331461DNABacillus fusiformis 1083 33atgagccatg atctgctggc ttcaatatca agtgcttcaa ttgctaacat tttaacagat 60caatcaacat tatttacttc agaaacaata aataacctct ctatttatgc tagtagagaa 120gggaaaactt catggccttt tgcggatgga gtaattgtaa ttgaagaaga ggcaaccgta 180aaatataaga tggcagttga atttaaaaga gttaatgaag gaattcacgg aattttaact 240gcattaggcc aatcacaagc gtatttaaaa aaaggttata atggaacaat cataataatt 300ccagaagtgt ataatactca tgaagcacct ggtgagtatt taaaaagtgt tcttgattta 360gttggtgaag atttaccaat aatgattttt acttataaaa taaatggaga aaatgattta 420gaagttaact gtatccgcaa tattgatctt tctacgacgg ctatcgattc tgacgatact 480accaatcaaa ctaatacaat tagtacacag tgggcacact tacgagaggg aagcacagag 540ccagatactt tttatagata tttacaaatg gcaaaaagaa tagatttaac agagttaaat 600gaacctacaa ttgaattccc tattgaactc ttaaacgcat tgccgaatga tgtagatcct 660ctgaaatact tatcaaatgc acctggggat acttatcatg attttgtttg gaggcacttt 720tggtttacat atatcattaa cgaaagaaca ttgcctttat ttactttaga aggggactta 780tacaaggtat gtgatgctag ttcttcttta ttaaaaaatg atggtttacc taagtatttt 840ttggtgggta aaagtaactc tccaaaaaat aaaattatag gaaaattaaa tgctggtacc 900attaacgagg aacaagcttg ggtagaatat gcccagaaaa taaaagacag agcacatagc 960tttagagaag atatagattc ttcactctat catataggta tgattgatga agatggaaag 1020cctacaagta taggttacaa atttgtagat gcttgtgaaa gaaatagaaa tgatagtata 1080aatggtactc cgctagctat ttttgaaaca gcaataatcc agcacgggga attaggtgca 1140tttattcatt acataagctt agcctcacaa aagattttta aagacactcc attaaagtat 1200agtgtgatag aaggaaatga atttaagtca tttaattcga ataattattt aaaagaagta 1260gaagaaatat tagctaatga tataaaagtt attagaaaag tttctttacg tggaggagtg 1320gggagaaaac ctttccaagc agaattagca gttttaggtt ttttaggttt ctttaaaaaa 1380ggtagaaata gatttaaacc tgttgttgga ttagatatcg attgggagaa agtatataca 1440gctttaaacc gagaaattta a 146134486PRTBacillus fusiformis 1083 34Met Ser His Asp Leu Leu Ala Ser Ile Ser Ser Ala Ser Ile Ala Asn 1 5 10 15 Ile Leu Thr Asp Gln Ser Thr Leu Phe Thr Ser Glu Thr Ile Asn Asn 20 25 30 Leu Ser Ile Tyr Ala Ser Arg Glu Gly Lys Thr Ser Trp Pro Phe Ala 35 40 45 Asp Gly Val Ile Val Ile Glu Glu Glu Ala Thr Val Lys Tyr Lys Met 50 55 60 Ala Val Glu Phe Lys Arg Val Asn Glu Gly Ile His Gly Ile Leu Thr 65 70 75 80 Ala Leu Gly Gln Ser Gln Ala Tyr Leu Lys Lys Gly Tyr Asn Gly Thr 85 90 95 Ile Ile Ile Ile Pro Glu Val Tyr Asn Thr His Glu Ala Pro Gly Glu 100 105 110 Tyr Leu Lys Ser Val Leu Asp Leu Val Gly Glu Asp Leu Pro Ile Met 115 120 125 Ile Phe Thr Tyr Lys Ile Asn Gly Glu Asn Asp Leu Glu Val Asn Cys 130 135 140 Ile Arg Asn Ile Asp Leu Ser Thr Thr Ala Ile Asp Ser Asp Asp Thr 145 150 155 160 Thr Asn Gln Thr Asn Thr Ile Ser Thr Gln Trp Ala His Leu Arg Glu 165 170 175 Gly Ser Thr Glu Pro Asp Thr Phe Tyr Arg Tyr Leu Gln Met Ala Lys 180 185 190 Arg Ile Asp Leu Thr Glu Leu Asn Glu Pro Thr Ile Glu Phe Pro Ile 195 200 205 Glu Leu Leu Asn Ala Leu Pro Asn Asp Val Asp Pro Leu Lys Tyr Leu 210 215 220 Ser Asn Ala Pro Gly Asp Thr Tyr His Asp Phe Val Trp Arg His Phe 225 230 235 240 Trp Phe Thr Tyr Ile Ile Asn Glu Arg Thr Leu Pro Leu Phe Thr Leu 245 250 255 Glu Gly Asp Leu Tyr Lys Val Cys Asp Ala Ser Ser Ser Leu Leu Lys 260 265 270 Asn Asp Gly Leu Pro Lys Tyr Phe Leu Val Gly Lys Ser Asn Ser Pro 275 280 285 Lys Asn Lys Ile Ile Gly Lys Leu Asn Ala Gly Thr Ile Asn Glu Glu 290 295 300 Gln Ala Trp Val Glu Tyr Ala Gln Lys Ile Lys Asp Arg Ala His Ser 305 310 315 320 Phe Arg Glu Asp Ile Asp Ser Ser Leu Tyr His Ile Gly Met Ile Asp 325 330 335 Glu Asp Gly Lys Pro Thr Ser Ile Gly Tyr Lys Phe Val Asp Ala Cys 340 345 350 Glu Arg Asn Arg Asn Asp Ser Ile Asn Gly Thr Pro Leu Ala Ile Phe 355 360 365 Glu Thr Ala Ile Ile Gln

His Gly Glu Leu Gly Ala Phe Ile His Tyr 370 375 380 Ile Ser Leu Ala Ser Gln Lys Ile Phe Lys Asp Thr Pro Leu Lys Tyr 385 390 395 400 Ser Val Ile Glu Gly Asn Glu Phe Lys Ser Phe Asn Ser Asn Asn Tyr 405 410 415 Leu Lys Glu Val Glu Glu Ile Leu Ala Asn Asp Ile Lys Val Ile Arg 420 425 430 Lys Val Ser Leu Arg Gly Gly Val Gly Arg Lys Pro Phe Gln Ala Glu 435 440 445 Leu Ala Val Leu Gly Phe Leu Gly Phe Phe Lys Lys Gly Arg Asn Arg 450 455 460 Phe Lys Pro Val Val Gly Leu Asp Ile Asp Trp Glu Lys Val Tyr Thr 465 470 475 480 Ala Leu Asn Arg Glu Ile 485 35870DNABacillus species lp 35atgttcgttc atggagataa tttaacgcaa aaagaaaatc atcgtacaaa atatacagat 60ggtttgtcta aacaatattt aacagaaata agagaaaaat ataatgaatg gaaaaaagcc 120aacgaagaat tgataggtcc ttttgctgag gcaacgcctg aagatgaagc aatagtgaaa 180aaaagagtag aattgctgaa tgattataaa gattttgtag accaacaaca ctatgcggaa 240aaatttgatt cacgttcgaa cctacattcc tcaattttag aagaatttgt ctactacctg 300tttaaggata tagcaaaaag ttttaatgat gaagccattg taggtaaatc acatgctttt 360aaagatttgt ttataaatcc tagtagttat aaagatatgg taactcaacc aaatgtaaag 420gtagaaatta aggaccatga ttttattatt ggtgtaggaa ttgaagcaaa aatgattgtc 480aaaggttcaa ctgaaattga aaatcatact ttagaagtag cggcggttgc gattgaatgt 540aaaacatatt tagataaaac aatgctagag ggttcatcag ttgccgcaga acaattgaaa 600agtaggaatc ctaacgcaaa atatattgta gtatcagaat ggttaaagct atctgaacaa 660gtaaaccttc agaaatataa agttgaccaa atttatgttt tgagaaaaca aaaaaatact 720gatagagaat ttagatatgc tgacacgtac gtgaaaaatg ctattcatga agatgtagtt 780ttacatttat tccatacaat aagattacac ttaactactg aatgggatgg gtctattagc 840catggtattg atagaggtta cctactatag 87036289PRTBacillus species lp 36Met Phe Val His Gly Asp Asn Leu Thr Gln Lys Glu Asn His Arg Thr 1 5 10 15 Lys Tyr Thr Asp Gly Leu Ser Lys Gln Tyr Leu Thr Glu Ile Arg Glu 20 25 30 Lys Tyr Asn Glu Trp Lys Lys Ala Asn Glu Glu Leu Ile Gly Pro Phe 35 40 45 Ala Glu Ala Thr Pro Glu Asp Glu Ala Ile Val Lys Lys Arg Val Glu 50 55 60 Leu Leu Asn Asp Tyr Lys Asp Phe Val Asp Gln Gln His Tyr Ala Glu 65 70 75 80 Lys Phe Asp Ser Arg Ser Asn Leu His Ser Ser Ile Leu Glu Glu Phe 85 90 95 Val Tyr Tyr Leu Phe Lys Asp Ile Ala Lys Ser Phe Asn Asp Glu Ala 100 105 110 Ile Val Gly Lys Ser His Ala Phe Lys Asp Leu Phe Ile Asn Pro Ser 115 120 125 Ser Tyr Lys Asp Met Val Thr Gln Pro Asn Val Lys Val Glu Ile Lys 130 135 140 Asp His Asp Phe Ile Ile Gly Val Gly Ile Glu Ala Lys Met Ile Val 145 150 155 160 Lys Gly Ser Thr Glu Ile Glu Asn His Thr Leu Glu Val Ala Ala Val 165 170 175 Ala Ile Glu Cys Lys Thr Tyr Leu Asp Lys Thr Met Leu Glu Gly Ser 180 185 190 Ser Val Ala Ala Glu Gln Leu Lys Ser Arg Asn Pro Asn Ala Lys Tyr 195 200 205 Ile Val Val Ser Glu Trp Leu Lys Leu Ser Glu Gln Val Asn Leu Gln 210 215 220 Lys Tyr Lys Val Asp Gln Ile Tyr Val Leu Arg Lys Gln Lys Asn Thr 225 230 235 240 Asp Arg Glu Phe Arg Tyr Ala Asp Thr Tyr Val Lys Asn Ala Ile His 245 250 255 Glu Asp Val Val Leu His Leu Phe His Thr Ile Arg Leu His Leu Thr 260 265 270 Thr Glu Trp Asp Gly Ser Ile Ser His Gly Ile Asp Arg Gly Tyr Leu 275 280 285 Leu 371077DNABacillus megaterium GC subgroup A 37ttgaactatt tctctttgca tcctaacgta tacgcaactg gtagaccaaa aggattaata 60aatatgttag aatccgtgtg gatatcaaac caaaaacccg gtgacgggac tatgtattta 120atttctggat ttgcaaatta taatggtgga ataagattct acgaaacatt tacagaacat 180attaaccatg gtggtaaagt tatcgccatt ttaggaggca gcacctccca aagattgtca 240agtaaacaag ttgtagcaga attggtatct cgaggtgtag atgtatacat cattaataga 300aaacgacttc ttcatgctaa actatatggt tccagcagta attctggaga atctttagta 360gtttcttctg gtaactttac tggtccaggc atgtctcaaa atgttgaagc ctcattattg 420ttagataata atacaacctc atcgatggga ttttcttgga atggtatggt caattcaatg 480cttgatcaga aatggcaaat tcataatttg agcaattcca accctacatc acctagttgg 540aatttattgt atgacgaacg cacaacaaat ctaactttag atgatactca gaaagtgacc 600ttaattctta ccttaggtca tgcggatacc gcaagaattc aggctgcacc aaaaagtaag 660gctggagagg gatctcaata cttttggtta agtaaagata gttatgactt ttttccacct 720ttaacaatcc gaaataaacg tgggactaaa gcaacttatt cttgccttat aaacatgaac 780tatttagaca taaaatatat tgatagcgaa tgtagagtca cttttgaagc agaaaacaat 840ttcgatttta ggttaggaac aggaaaactt agatacacaa atgtagcagc aagtgatgac 900atagctgcaa ttactcgtgt aggtgattca gattatgaat taagaataat taaaaaagga 960agttctaatt atgatgcact tgattcagct gcagtaaatt ttataggtaa tagaggaaaa 1020agatacggat acatacctaa tgatgagttt gggagaatca taggagctaa gttttga 107738357PRTBacillus megaterium GC subgroup A 38Met Asn Tyr Phe Ser Leu His Pro Asn Val Tyr Ala Thr Gly Arg Pro 1 5 10 15 Lys Gly Leu Ile Asn Met Leu Glu Ser Val Trp Ile Ser Asn Gln Lys 20 25 30 Pro Gly Asp Gly Thr Met Tyr Leu Ile Ser Gly Phe Ala Asn Tyr Asn 35 40 45 Gly Gly Ile Thr Phe Tyr Glu Thr Phe Thr Glu His Ile Asn His Gly 50 55 60 Gly Lys Val Ile Ala Ile Leu Gly Gly Ser Thr Ser Gln Arg Leu Ser 65 70 75 80 Ser Lys Gln Val Val Ala Glu Leu Val Ser Arg Gly Val Asp Val Tyr 85 90 95 Ile Ile Asn Arg Lys Arg Leu Leu His Ala Lys Leu Tyr Gly Ser Ser 100 105 110 Ser Asn Ser Gly Glu Ser Leu Val Val Ser Ser Gly Asn Phe Thr Gly 115 120 125 Pro Gly Met Ser Gln Asn Val Glu Ser Ser Leu Leu Leu Tyr Asn Asn 130 135 140 Thr Thr Ser Ser Met Gly Phe Ser Trp Asn Gly Met Val Asn Ser Met 145 150 155 160 Leu Asp Gln Lys Trp Gln Ile His Asn Leu Ser Asn Ser Asn Pro Thr 165 170 175 Ser Pro Ser Trp Asn Leu Leu Tyr Asp Glu Arg Thr Thr Asn Thr Leu 180 185 190 Asp Asp Thr Gln Lys Val Thr Phe Ile Leu Thr Leu Cys His Ala Asp 195 200 205 Thr Ala Arg Ile Gln Ala Ala Pro Lys Ile Lys Ala Gly Glu Gly Ser 210 215 220 Gln Tyr Phe Trp Leu Ser Lys Asp Ser Tyr Asp Phe Phe Pro Pro Leu 225 230 235 240 Thr Ile Arg Asn Lys Arg Gly Thr Lys Ala Thr Tyr Ser Cys Leu Ile 245 250 255 Asn Met Asn Tyr Leu Asp Ile Lys Tyr Ile Asp Ser Glu Cys Arg Val 260 265 270 Thr Phe Glu Ala Glu Asn Asn Phe Asp Phe Arg Leu Gly Thr Gly Lys 275 280 285 Leu Ile Tyr Thr Asn Val Ala Ala Ser Asp Asp Ile Ala Ala Ile Thr 290 295 300 Arg Val Gly Asp Ser Asp Tyr Glu Leu Arg Ile Ile Lys Lys Gly Ser 305 310 315 320 Ser Asn Tyr Asp Ala Leu Asp Ser Ala Ala Val Asn Phe Ile Gly Asn 325 330 335 Arg Gly Lys Arg Tyr Gly Tyr Ile Pro Asn Asp Glu Phe Gly Arg Ile 340 345 350 Ile Gly Ala Lys Phe 355 39924DNABacillus stearothermophilus J695 39atgacttttg ataaaattgc agtcaaacag atcttgttaa ggcttctaaa aggtgaagat 60tatagaggag aagtacttaa cattattaat gctgactttt tagactttgc tttgcagttt 120tttaaagatg tcgctttagc aaaacttcaa aatgaagagt taaccgatga ttggtataaa 180aaatatttta ttcaaaatcc atctctcaca aaagaaaagg ttgctattta ctcaggttta 240aacatgaaga caataagtaa tacctataaa actacagcaa agaatgtagt tgttgatgcg 300tcattagagc attacgatgc atttgtaaaa acgatccaag aattaataga aattgatgat 360tctttagaac taatgttaac tattaagtat aacaaggtta gtgttgaact tactcttagt 420gagtctttaa tagtaatgaa tgtattagca gttaaaaggg cagctattag aggaggagca 480tggagtacag cgggaaaacg agttgaaaaa cttttaatgc taacattgtg taagctattt 540agggtaccgg ataaacatta taaaagtatt tatgtagcgc aattaaaaga tgagaacgat 600tttagtagag aaattgattt ttatttgatt gaccaaaaca acaatgaatt aaaatgcgag 660gtcaaattaa tgggaaaagg aaatccagaa agtgctgatg cggtaatcgc tcgtgacagt 720aagatttttg tagcagatac attatcagaa acaaataaga aacaattaga ttttttaaaa 780gttgagtggg ttgagcttag aagcgaaaaa ggctatgaaa aatttaaaac tattctttct 840aacagaggaa ttccatatga agatatagaa gaaatcactc cagaatatct agaaaaagtc 900attgatgagt ctttaggaat ttaa 92440306PRTBacillus stearothermophilus J695 40Met Thr Phe Asp Lys Ile Ala Val Lys Gln Ile Leu Leu Arg Leu Leu 1 5 10 15 Lys Gly Glu Asp Tyr Arg Gly Glu Val Leu Asn Ile Ile Asn Ala Asp 20 25 30 Phe Leu Asp Phe Ala Leu Gln Phe Phe Lys Asp Val Ala Leu Ala Lys 35 40 45 Leu Gln Asn Glu Glu Leu Thr Asp Asp Trp Tyr Lys Lys Tyr Phe Ile 50 55 60 Gln Asn Pro Ser Leu Thr Lys Glu Lys Val Ala Ile Tyr Ser Gly Leu 65 70 75 80 Asn Met Lys Thr Ile Ser Asn Thr Tyr Lys Thr Thr Ala Lys Asn Val 85 90 95 Val Val Asp Ala Ser Leu Glu His Tyr Asp Ala Phe Val Lys Thr Ile 100 105 110 Gln Glu Leu Ile Glu Ile Asp Asp Ser Leu Glu Leu Met Leu Thr Ile 115 120 125 Lys Tyr Asn Lys Val Ser Val Glu Leu Thr Leu Ser Glu Ser Leu Ile 130 135 140 Val Met Asn Val Leu Ala Val Lys Arg Ala Ala Ile Arg Gly Gly Ala 145 150 155 160 Trp Ser Thr Ala Gly Lys Arg Val Glu Lys Leu Leu Met Leu Thr Leu 165 170 175 Cys Lys Leu Phe Arg Val Pro Asp Lys His Tyr Lys Ser Ile Val Ala 180 185 190 Gln Leu Lys Asp Glu Asn Asp Phe Ser Arg Glu Ile Asp Phe Tyr Leu 195 200 205 Ile Asp Gln Asn Asn Asn Glu Leu Lys Cys Glu Val Lys Leu Met Gly 210 215 220 Lys Gly Asn Pro Glu Ser Ala Asp Ala Val Ile Ala Arg Asp Ser Lys 225 230 235 240 Ile Phe Val Ala Asp Thr Leu Ser Glu Thr Asn Lys Lys Gln Leu Asp 245 250 255 Phe Leu Lys Val Glu Trp Val Glu Leu Arg Ser Glu Lys Gly Tyr Glu 260 265 270 Lys Phe Lys Thr Ile Leu Ser Asn Arg Gly Ile Pro Tyr Glu Asp Ile 275 280 285 Glu Glu Ile Thr Pro Glu Tyr Leu Glu Lys Val Ile Asp Glu Ser Leu 290 295 300 Gly Ile 305 411374DNABacillus smithii 41atgaggtaca atccagcaga gcaatttaga tgtactatta ttagaggaaa agcgaaaaat 60gctcttgata acctattgcc agcttatgcc aagatcattt ctgatatttg cccatgtagt 120aagaaagagt tcccttcggc attcaatcaa aggcttaacg aggttcttgg tgagagcacg 180aaaaaaactt tagataatca cagaacagaa attgctggta aattatttgg aatgttttat 240gaggatgaca atgggatagt tttttcttct gagagaactg aaaagtattt aaaggactca 300gaccagccag ctttcttcaa agatttgtgt tttaagttcc agtttcccaa tggtatggat 360aagatagata acgtattaga aaaaatgcga tttaaaatat caattaggca gtttccttat 420attcttcagc ttttattgtt agctagtgaa aaaggaatta aattaacaaa ggatgaagta 480gggtattatg ttttaaattc tcttcacgtt ttacaagggc aaatacatcc gactattgta 540ttggaacaaa tcattgctga tcgaagggca ggaaatataa agaaagtaat ggttcctggt 600aaagcctctt cttattctgt acagcatata aacgagcaat taaactattt agaattggct 660aacttgatta ggattgatga taaagttatt tccattaatt ttaaggaatc tgaaacaatt 720gaattgatgg catctttttg gaataaaaag cctgagttcg atgcatataa atacaatttg 780gaagatagag aacaaagaaa aagattttat aaggattggc aattatatta ttctaattta 840aataaagtga aagagttcca aacgacagtt gaatccctta acatctcact tgatacttct 900actccttcta cccatattga taaaactgct attggggatg aaggagagaa ttttgtttta 960gaatatgaga agaaaagagt tagtaagttc gacccaagat tggtacaaaa ggtagtacct 1020ttaggtaaaa ccagaggatt agggtatgat attcaatctg tgattgctga gcctggagaa 1080aatgctgagt tcgttaagta tattgaggtt aaaacaacta agcgagttac tgtcccagat 1140gtcaatgatc caacttggat tgataccatt aatttaacta gaaatgagtg gattgcagct 1200gcacaacata gagagttcta ttctgtatac agggtttatc taactccaga aagggttact 1260gtatttgtaa taaatgatcc atttactaaa aataaagaca acataattaa atgcaaacct 1320ttaacatata ggttagattt ttcacacgta gcaattgata atgttttgca atag 137442457PRTBacillus smithii 42Met Arg Tyr Asn Pro Ala Glu Gln Phe Arg Cys Thr Ile Ile Arg Gly 1 5 10 15 Lys Ala Lys Asn Ala Leu Asp Asn Leu Leu Pro Ala Tyr Ala Lys Ile 20 25 30 Ile Ser Asp Ile Cys Pro Cys Ser Lys Lys Glu Phe Pro Ser Ala Phe 35 40 45 Asn Gln Arg Leu Asn Glu Val Leu Gly Glu Ser Thr Lys Lys Thr Leu 50 55 60 Asp Asn His Arg Thr Glu Ile Ala Gly Lys Leu Phe Gly Met Phe Tyr 65 70 75 80 Glu Asp Asp Asn Gly Ile Val Phe Ser Ser Glu Arg Thr Glu Lys Tyr 85 90 95 Leu Lys Asp Ser Asp Gln Pro Ala Phe Phe Lys Asp Leu Cys Phe Lys 100 105 110 Phe Gln Phe Pro Asn Gly Met Asp Lys Ile Asp Asn Val Leu Glu Lys 115 120 125 Met Arg Phe Lys Ile Ser Ile Arg Gln Phe Pro Tyr Ile Leu Gln Leu 130 135 140 Leu Leu Leu Ala Ser Glu Lys Gly Ile Lys Leu Thr Lys Asp Glu Val 145 150 155 160 Gly Tyr Tyr Val Leu Asn Ser Leu His Val Leu Gln Gly Gln Ile His 165 170 175 Pro Thr Ile Val Leu Glu Gln Ile Ile Ala Asp Arg Arg Ala Gly Asn 180 185 190 Ile Lys Lys Val Met Val Pro Gly Lys Ala Ser Ser Tyr Ser Val Gln 195 200 205 His Ile Asn Glu Gln Leu Asn Tyr Leu Glu Leu Ala Asn Leu Ile Arg 210 215 220 Ile Asp Asp Lys Val Ile Ser Ile Asn Phe Lys Glu Ser Glu Thr Ile 225 230 235 240 Glu Leu Met Ala Ser Phe Trp Asn Lys Lys Pro Glu Phe Asp Ala Tyr 245 250 255 Lys Tyr Asn Leu Glu Asp Arg Glu Gln Arg Lys Arg Phe Tyr Lys Asp 260 265 270 Trp Gln Leu Tyr Tyr Ser Asn Leu Asn Lys Val Lys Glu Phe Gln Thr 275 280 285 Thr Val Glu Ser Leu Asn Ile Ser Leu Asp Thr Ser Thr Pro Ser Thr 290 295 300 His Ile Asp Lys Thr Ala Ile Gly Asp Glu Gly Glu Asn Phe Val Leu 305 310 315 320 Glu Tyr Glu Lys Lys Arg Val Ser Lys Phe Asp Pro Arg Leu Val Gln 325 330 335 Lys Val Val Pro Leu Gly Lys Thr Arg Gly Leu Gly Tyr Asp Ile Gln 340 345 350 Ser Val Ile Ala Glu Pro Gly Glu Asn Ala Glu Phe Val Lys Tyr Ile 355 360 365 Glu Val Lys Thr Thr Lys Arg Val Thr Val Pro Asp Val Asn Asp Pro 370 375 380 Thr Trp Ile Asp Thr Ile Asn Leu Thr Arg Asn Glu Trp Ile Ala Ala 385 390 395 400 Ala Gln His Arg Glu Phe Tyr Ser Val Tyr Arg Val Tyr Leu Thr Pro 405 410 415 Glu Arg Val Thr Val Phe Val Ile Asn Asp Pro Phe Thr Lys Asn Lys 420 425 430 Asp Asn Ile Ile Lys Cys Lys Pro Leu Thr Tyr Arg Leu Asp Phe Ser 435 440 445 His Val Ala Ile Asp Asn Val Leu Gln 450 455 431542DNABacillus species 2521 43atgaaattgg gtgaaataaa tctaaaaaag tttttggagg aaaaaaaagg aatagtttac 60ggcgaactcg ttcaagatgc taaactacgc tggtatacga gagaatatga atatgcgata 120ttgaaagata ataaaatgga gatatggccg aagggtaaag tagcaaataa aatcgttcta 180ccaaccaaaa taattttgga ttcagaattg gttaccttct ttggattata tagtggtgac 240ggcgcaaaag gcacggaaat tataaataaa cccgggagaa ttacaacttc tatctctttt 300tctcaaaagg aacctcattt aattaaattt gctataaatc aattcaggaa aatttttggg 360gataatattt ggtttgattt ttctttaggt gaggacagtg cttatttcat ggatgaggat 420gggcataata gaattaaatc tgttctaaat gatgatgtac cattggtaat ggagtctctt

480aatgaattaa atgttaattt aagtgcggca gatataatat atttaaatga gcaaaggaat 540gtttcaatta ctaacgaaga agccttggca tttcattatc aatataataa tgaaatgcaa 600aaatatttaa tagatgtaaa aatgaatgat ttaaatgatg ttggaattac acttggtcct 660aatgaccgag taaatgcatc tttacgtcgg ccattcaaaa aaggcgcaag aacaatgggg 720ggaagtagca gatctgatga actctatgtt aaaggggttt ctttatttgg ggagctattt 780ttaaaaattc tccatagtat agaggaatct attttgaatg atacacaaga atcaacagac 840actttaataa aatgggatgg taaaccatct acgatagggg aagttattga cctaaaaaat 900cactttttgg aaagtcctta tgcagaaatt aatggttcta agccaatatt agaagaggaa 960gcactctacc taattggaaa atatccaaga ggttcgttgg tgaaattaaa taaacggttg 1020cgtcaaactc cattatggct gtatgctgcg gggctttatt tagcagaggg atctactgca 1080aaagaaaaaa tgtttcagat gtatacaagt agagctagag ggctatcact aagctttact 1140tcttctgaac cgtatagcct agaaattata attaaagcgt tagagctatt atttttcgac 1200gagcaaattt taagtagctg gaaagtaaaa gttggatccc agtattttcc tgaactagtc 1260accacagggt taaaacttgg tgtccctatg ttaagggggg ggctaagtgg tgacgggaag 1320ttgagaacta tggaaatttc acttagtatt aagagatggg cattggagat tgtacccttt 1380ttcagcaaat atgaggatag gtttagccat gttgaaccta caggcgcagg ggtagcaaga 1440atagattttt caggatcatc aaaactatgt aaatggtatt ttgggttaat aatttattcg 1500gcatttaaga atactactaa agatccaaaa ggggaatttt aa 154244513PRTBacillus species 2521 44Met Lys Leu Gly Glu Ile Asn Leu Lys Lys Phe Leu Glu Glu Lys Lys 1 5 10 15 Gly Ile Val Tyr Gly Glu Leu Val Gln Asp Ala Lys Leu Arg Trp Tyr 20 25 30 Thr Arg Glu Tyr Glu Tyr Ala Ile Leu Lys Asp Asn Lys Met Glu Ile 35 40 45 Trp Pro Lys Gly Lys Val Ala Asn Lys Ile Val Leu Pro Thr Lys Ile 50 55 60 Ile Leu Asp Ser Glu Leu Val Thr Phe Phe Gly Leu Tyr Ser Gly Asp 65 70 75 80 Gly Ala Lys Gly Thr Glu Ile Ile Asn Lys Pro Gly Arg Ile Thr Thr 85 90 95 Ser Ile Ser Phe Ser Gln Lys Glu Pro His Leu Ile Lys Phe Ala Ile 100 105 110 Asn Gln Phe Arg Lys Ile Phe Gly Asp Asn Ile Trp Phe Asp Phe Ser 115 120 125 Leu Gly Glu Asp Ser Ala Tyr Phe Met Asp Glu Asp Gly His Asn Arg 130 135 140 Ile Lys Ser Val Leu Asn Asp Asp Val Pro Leu Val Met Glu Ser Leu 145 150 155 160 Asn Glu Leu Asn Val Asn Leu Ser Ala Ala Asp Ile Ile Tyr Leu Asn 165 170 175 Glu Gln Arg Asn Val Ser Ile Thr Asn Glu Glu Ala Leu Ala Phe His 180 185 190 Tyr Gln Tyr Asn Asn Glu Met Gln Lys Tyr Leu Ile Asp Val Lys Met 195 200 205 Asn Asp Leu Asn Asp Val Gly Ile Thr Leu Gly Pro Asn Asp Arg Val 210 215 220 Asn Ala Ser Leu Arg Arg Pro Phe Lys Lys Gly Ala Arg Thr Met Gly 225 230 235 240 Gly Ser Ser Arg Ser Asp Glu Leu Tyr Val Lys Gly Val Ser Leu Phe 245 250 255 Gly Glu Leu Phe Leu Lys Ile Leu His Ser Ile Glu Glu Ser Ile Leu 260 265 270 Asn Asp Thr Gln Glu Ser Thr Asp Thr Leu Ile Lys Trp Asp Gly Lys 275 280 285 Pro Ser Thr Ile Gly Glu Val Ile Asp Leu Lys Asn His Phe Leu Glu 290 295 300 Ser Pro Tyr Ala Glu Ile Asn Gly Ser Lys Pro Ile Leu Glu Glu Glu 305 310 315 320 Ala Leu Tyr Leu Ile Gly Lys Tyr Pro Arg Gly Ser Leu Val Lys Leu 325 330 335 Asn Lys Arg Leu Arg Gln Thr Pro Leu Trp Leu Tyr Ala Ala Gly Leu 340 345 350 Tyr Leu Ala Glu Gly Ser Thr Ala Lys Glu Lys Met Phe Gln Met Tyr 355 360 365 Thr Ser Arg Ala Arg Gly Leu Ser Leu Ser Phe Thr Ser Ser Glu Pro 370 375 380 Tyr Ser Leu Glu Ile Ile Ile Lys Ala Leu Glu Leu Leu Phe Phe Asp 385 390 395 400 Glu Gln Ile Leu Ser Ser Trp Lys Val Lys Val Gly Ser Gln Tyr Phe 405 410 415 Pro Glu Leu Val Thr Thr Gly Leu Lys Leu Gly Val Pro Met Leu Arg 420 425 430 Gly Gly Leu Ser Gly Asp Gly Lys Leu Arg Thr Met Glu Ile Ser Leu 435 440 445 Ser Ile Lys Arg Trp Ala Leu Glu Ile Val Pro Phe Phe Ser Lys Tyr 450 455 460 Glu Asp Arg Phe Ser His Val Glu Pro Thr Gly Ala Gly Val Ala Arg 465 470 475 480 Ile Asp Phe Ser Gly Ser Ser Lys Leu Cys Lys Trp Tyr Phe Gly Leu 485 490 495 Ile Ile Tyr Ser Ala Phe Lys Asn Thr Thr Lys Asp Pro Lys Gly Glu 500 505 510 Phe 453108DNABacillus sphaericus GC subgroup 45atgaatagag tagaatctaa aaaaaaatta gaacaattag ttcaacagtt cgagaagtat 60gaaagtacat atagcgcttc ggattataaa gaggcaactt taagatctag ttttttagat 120cccttttttg aactttttgg atgggaaatg cgccctgaaa gaataactaa tccagcagac 180ttagaagtga ttatagaaga aagtttagaa acggaaaaat ctactaagta tatagattat 240gtttttaaaa ttaatagaac gactcagttt ttggtagaag ctaaaaagcc agctgaaagt 300ctttctaaaa aagatcatat ttttcaggct aaaagttatg catttactac ggagattcca 360tttgtcattt taacaaattt taaagagttc agattttatg acgtttcaac tgaaccttta 420cacaatcaac cggatacaga taaagtggaa gaatattgtt ttgattataa agaatatgtt 480caaaactttg ataagttatg ggaattattc agcagagaag cagttgctaa cagaagttta 540gccaagtttt atgctaaaag aagaaatata gtagatagtc cagatttaat ttttaaactt 600aattatcaaa ttgataaagg tgcatcatta ctggatatat ctttcttaaa aaatttgaaa 660atatggagaa aatcattagc tgaaaatatc tttaataata attcacttaa tgttaacgta 720attaatgaag tagttcagag aatattagat agactgatat ttatccgtat cattgaagac 780agaaatattg aatctaaaga gtttttaaaa gaaattgtag aaatgcacga acaagataat 840tcgatttcag tgaaaaatga actagataaa ctatgtattg aattaaataa gaaatttaat 900gggttagttt tccatgacca cacatttgtt aacgaagcgt tgatagataa cgaaatttta 960atagttatta ttgacaattt atattatcca aagtctcctt ataactttag attaattaag 1020ccagaaattt taggacgaat attcgagcaa tttttgggtg aaaaaattga aataatcgat 1080ggaaaaataa cgttaggatt aaaagatatt aataaaaaat caggaggtgt ttattataca 1140ccttcatata tagttgaaaa aatagtagaa aatacattat ccaaaaaatt acataatgat 1200attactattg aaaatttaga acagataaaa atagctgaca tagcttgtgg ttcaggaagc 1260tttttaattt catcatataa atatttaatt gataaatttc aatatattta ttccaaatgt 1320tcggaagcgg atgttcaaac attaattagt aataacttag tatttataga caatggtaaa 1380ttaatgttaa caatggaaca taaaaagggg atacttcagc aaaatatttt tggggtagat 1440atagattcac aagcaattca ggtagcgaaa ttaagtcttt atataaccat gttagaagaa 1500ggatacagag aaggtacatt aagacctata ttaccagact taaatgataa tattaaacat 1560ggtaactcaa taatagataa tgaaatttta tttgaagatg atataaatta cgatattgat 1620gcaacattac cattcgattg ggaatatgct tttcctgata ttatagataa cggaggtttt 1680gatgtaatat taggcaatcc accctatata agaattcaaa tttttgaaga gttatatgga 1740aaagatgtag ttaattattt gaaaaaaaaa tacgtttctg ccgaaaaatt taactttgat 1800atatatgtcg tgtttataga aaaagcattg tcactcttga atgaccaggg gatattggga 1860tatattgtga tgaacaaatt ttttactaca caatatggag aaaaattgcg cgagttaata 1920acttcacaaa aattattata tgaaatcatt gattttggaa ttaatgaaat atttaataat 1980gctactactt atacttgtat attaatttta gacaaaacta atccagatga aataattatt 2040gaaagagtga ttgatttaaa tacttggaaa gctggagaat cttcagatcg gaaagtggta 2100gatcataccg aattcactag tactccttgg tatttatcaa gcaatactga tgaagaaatt 2160tacaaattct ttgaagaaaa tatggtttta cttgaaacca ttagtgatag ggtttttgtt 2220ggtgttcaga cagactgtga tccagtatat attttagaag aagtttatga agaagaaaat 2280tatttatatt gtaagtcaga atatactact gaagtacaca agtttgaaaa agatcattta 2340aaaccatttt taaaaggttc tctagatata aagaaatata ctttttcaaa tgttaataag 2400tggttacttt tcccttatac caattcggaa aatacttctg atttaattcc cgaaacaact 2460tacaaacagt atttcccaga aacatggaaa tacttagagt cttgtaaaga aagattagca 2520aaaagaaaaa gtattgaaag agaattggat attaatccga attataatga gtggtataaa 2580tatatttaca aaaagaatca cacgaggatg gaccaattaa aaatagtatt tcctgcgata 2640tcgaagggta gtagcttttg ttatgattcg gatggagagt actattttgt aggaagtggt 2700gctggaggcg gtggtggagg cgcaatagtc ttgccagatc aatctgatta taattattta 2760tccttacttg gaattctaaa ttcagaagta gtttcatatc aaattgtaag aagaggttca 2820aaacataaag gttcttatta tggtgtagat aaaaagagaa tagaaaatct atatgtgcca 2880ttgattaatg aggataataa aaatttattt agtaatattt caaaaatggt agctcaaatt 2940cttgatgcgt ttcaaaaaat gcatcaagca gggacaacgg atgttggtaa agaacaactt 3000caacaaagaa taaaaatgct taatgctaga ataaatgagc tggtatatag actgtataat 3060ttaccagtag aatataaaga atatattaaa aatgccttag aaaattaa 3108461035PRTBacillus sphaericus GC subgroup 46Met Asn Arg Val Glu Ser Lys Lys Lys Leu Glu Gln Leu Val Gln Gln 1 5 10 15 Phe Glu Lys Tyr Glu Ser Thr Tyr Ser Ala Ser Asp Tyr Lys Glu Ala 20 25 30 Thr Leu Arg Ser Ser Phe Leu Asp Pro Phe Phe Glu Leu Phe Gly Trp 35 40 45 Glu Met Arg Pro Glu Arg Ile Thr Asn Pro Ala Asp Leu Glu Val Ile 50 55 60 Ile Glu Glu Ser Leu Glu Thr Glu Lys Ser Thr Lys Tyr Ile Asp Tyr 65 70 75 80 Val Phe Lys Ile Asn Arg Thr Thr Gln Phe Leu Val Glu Ala Lys Lys 85 90 95 Pro Ala Glu Ser Leu Ser Lys Lys Asp His Ile Phe Gln Ala Lys Ser 100 105 110 Tyr Ala Phe Thr Thr Glu Ile Pro Phe Val Ile Leu Thr Asn Phe Lys 115 120 125 Glu Phe Arg Phe Tyr Asp Val Ser Thr Glu Pro Leu His Asn Gln Pro 130 135 140 Asp Thr Asp Lys Val Glu Glu Tyr Cys Phe Asp Tyr Lys Glu Tyr Val 145 150 155 160 Gln Asn Phe Asp Lys Leu Trp Glu Leu Phe Ser Arg Glu Ala Val Ala 165 170 175 Asn Arg Ser Leu Ala Lys Phe Tyr Ala Lys Arg Arg Asn Ile Val Asp 180 185 190 Ser Pro Asp Leu Ile Phe Lys Leu Asn Tyr Gln Ile Asp Lys Gly Ala 195 200 205 Ser Leu Leu Asp Ile Ser Phe Leu Lys Asn Leu Lys Ile Trp Arg Lys 210 215 220 Ser Leu Ala Glu Asn Ile Phe Asn Asn Asn Ser Leu Asn Val Asn Val 225 230 235 240 Ile Asn Glu Val Val Gln Arg Ile Leu Asp Arg Leu Ile Phe Ile Arg 245 250 255 Ile Ile Glu Asp Arg Asn Ile Glu Ser Lys Glu Phe Leu Lys Glu Ile 260 265 270 Val Glu Met His Glu Gln Asp Asn Ser Ile Ser Val Lys Asn Glu Leu 275 280 285 Asp Lys Leu Cys Ile Glu Leu Asn Lys Lys Phe Asn Gly Leu Val Phe 290 295 300 His Asp His Thr Phe Val Asn Glu Ala Leu Ile Asp Asn Glu Ile Leu 305 310 315 320 Ile Val Ile Ile Asp Asn Leu Tyr Tyr Pro Lys Ser Pro Tyr Asn Phe 325 330 335 Arg Leu Ile Lys Pro Glu Ile Leu Gly Arg Ile Phe Glu Gln Phe Leu 340 345 350 Gly Glu Lys Ile Glu Ile Ile Asp Gly Lys Ile Thr Leu Gly Leu Lys 355 360 365 Asp Ile Asn Lys Lys Ser Gly Gly Val Tyr Tyr Thr Pro Ser Tyr Ile 370 375 380 Val Glu Lys Ile Val Glu Asn Thr Leu Ser Lys Lys Leu His Asn Asp 385 390 395 400 Ile Thr Ile Glu Asn Leu Glu Gln Ile Lys Ile Ala Asp Ile Ala Cys 405 410 415 Gly Ser Gly Ser Phe Leu Ile Ser Ser Tyr Lys Tyr Leu Ile Asp Lys 420 425 430 Phe Gln Tyr Ile Tyr Ser Lys Cys Ser Glu Ala Asp Val Gln Thr Leu 435 440 445 Ile Ser Asn Asn Leu Val Phe Ile Asp Asn Gly Lys Leu Met Leu Thr 450 455 460 Met Glu His Lys Lys Gly Ile Leu Gln Gln Asn Ile Phe Gly Val Asp 465 470 475 480 Ile Asp Ser Gln Ala Ile Gln Val Ala Lys Leu Ser Leu Tyr Ile Thr 485 490 495 Met Leu Glu Glu Gly Tyr Arg Glu Gly Thr Leu Arg Pro Ile Leu Pro 500 505 510 Asp Leu Asn Asp Asn Ile Lys His Gly Asn Ser Ile Ile Asp Asn Glu 515 520 525 Ile Leu Phe Glu Asp Asp Ile Asn Tyr Asp Ile Asp Ala Thr Leu Pro 530 535 540 Phe Asp Trp Glu Tyr Ala Phe Pro Asp Ile Ile Asp Asn Gly Gly Phe 545 550 555 560 Asp Val Ile Leu Gly Asn Pro Pro Tyr Ile Arg Ile Gln Ile Phe Glu 565 570 575 Glu Leu Tyr Gly Lys Asp Val Val Asn Tyr Leu Lys Lys Lys Tyr Val 580 585 590 Ser Ala Glu Lys Phe Asn Phe Asp Ile Tyr Val Val Phe Ile Glu Lys 595 600 605 Ala Leu Ser Leu Leu Asn Asp Gln Gly Ile Leu Gly Tyr Ile Val Met 610 615 620 Asn Lys Phe Phe Thr Thr Gln Tyr Gly Glu Lys Leu Arg Glu Leu Ile 625 630 635 640 Thr Ser Gln Lys Leu Leu Tyr Glu Ile Ile Asp Phe Gly Ile Asn Glu 645 650 655 Ile Phe Asn Asn Ala Thr Thr Tyr Thr Cys Ile Leu Ile Leu Asp Lys 660 665 670 Thr Asn Pro Asp Glu Ile Ile Ile Glu Arg Val Ile Asp Leu Asn Thr 675 680 685 Trp Lys Ala Gly Glu Ser Ser Asp Arg Lys Val Val Asp His Thr Glu 690 695 700 Phe Thr Ser Thr Pro Trp Tyr Leu Ser Ser Asn Thr Asp Glu Glu Ile 705 710 715 720 Tyr Lys Phe Phe Glu Glu Asn Met Val Leu Leu Glu Thr Ile Ser Asp 725 730 735 Arg Val Phe Val Gly Val Gln Thr Asp Cys Asp Pro Val Tyr Ile Leu 740 745 750 Glu Glu Val Tyr Glu Glu Glu Asn Tyr Leu Tyr Cys Lys Ser Glu Tyr 755 760 765 Thr Thr Glu Val His Lys Phe Glu Lys Asp His Leu Lys Pro Phe Leu 770 775 780 Lys Gly Ser Leu Asp Ile Lys Lys Tyr Thr Phe Ser Asn Val Asn Lys 785 790 795 800 Trp Leu Leu Phe Pro Tyr Thr Asn Ser Glu Asn Thr Ser Asp Leu Ile 805 810 815 Pro Glu Thr Thr Tyr Lys Gln Tyr Phe Pro Glu Thr Trp Lys Tyr Leu 820 825 830 Glu Ser Cys Lys Glu Arg Leu Ala Lys Arg Lys Ser Ile Glu Arg Glu 835 840 845 Leu Asp Ile Asn Pro Asn Tyr Asn Glu Trp Tyr Lys Tyr Ile Tyr Lys 850 855 860 Lys Asn His Thr Arg Met Asp Gln Leu Lys Ile Val Phe Pro Ala Ile 865 870 875 880 Ser Lys Gly Ser Ser Phe Cys Tyr Asp Ser Asp Gly Glu Tyr Tyr Phe 885 890 895 Val Gly Ser Gly Ala Gly Gly Gly Gly Gly Gly Ala Ile Val Leu Pro 900 905 910 Asp Gln Ser Asp Tyr Asn Tyr Leu Ser Leu Leu Gly Ile Leu Asn Ser 915 920 925 Glu Val Val Ser Tyr Gln Ile Val Arg Arg Gly Ser Lys His Lys Gly 930 935 940 Ser Tyr Tyr Gly Val Asp Lys Lys Arg Ile Glu Asn Leu Tyr Val Pro 945 950 955 960 Leu Ile Asn Glu Asp Asn Lys Asn Leu Phe Ser Asn Ile Ser Lys Met 965 970 975 Val Ala Gln Ile Leu Asp Ala Phe Gln Lys Met His Gln Ala Gly Thr 980 985 990 Thr Asp Val Gly Lys Glu Gln Leu Gln Gln Arg Ile Lys Met Leu Asn 995 1000 1005 Ala Arg Ile Asn Glu Leu Val Tyr Arg Leu Tyr Asn Leu Pro Val 1010 1015 1020 Glu Tyr Lys Glu Tyr Ile Lys Asn Ala Leu Glu Asn 1025 1030 1035 472757DNABacillus species 1310 47atgaaaaaag tgggagcaac gcgtgataat gaacgtagtt gggctattga tctaatatca 60aggattaatt caggtgccat tgtttgtaaa gaagatagta tgatacaaca tgcaggagga 120gagatggggc tatcaacagg tagcggctct cttttccctg atgttctttt atttggagat 180aagggcaaaa caagggtctt acaaggttgg gaattaaaat accctgatac accaatagat 240gatagagaat tatttataaa tgctgtaaaa aaggcagagc ttctaggagt aaacagtttc 300ttattatgga atgtatcggt tgctcatcta tacgttaaaa atgaagaaag tggaaaatat 360gaactattaa agaaatggga tgacctaaaa catattacta aacgttctga agttgcttat 420agtatggctg aaataaatca agtgttagaa agtatcttga aggatttaga atactacttt 480cgaaatggta cattacgtac cgaaaaaata cttaactcaa ttgttaatga gcaaatgctc 540tcgttggcgt tcaataatat tgaagattgt gcactttcat taaaaaatgc ttctgcaaaa

600gatagtgatt ttaatgatga aataatactt tggtgggaaa cagaaggctt gagctatgga 660aagaaagctg ataggtggat tgagttatca agattagcta tcatttcgct tatgaataaa 720ttgatatttg caaatattct aaagaagtat aattctcatg cacagattat agatcaggtt 780aacgattcac ttacagtaga agagtgccta gatatcctta atgaaatatc tgagaagtgt 840gacttttata atatttttga agaaaaacca ggggaaagat atattgatgt agctacgtta 900aaagtactaa caaactttaa tgattatatt atgaatttag atttcaatag ttatagtgat 960aggttacttg aagaattatt aaatattgta gtgacaagaa gtaagcggaa agttgctggt 1020caattctcaa caccaaaaga attagctatg attttgacct ctttaacgat gacggataaa 1080tcatcaagaa tttctgatcc gtgttgtgga acaggaacaa ttgtaaaagc agcttatgat 1140cttaaattgg tttcgggtat tgatagcagc gatgctatag atcaaatttg ggcaggcgat 1200aaatttagat atccactaca gtttgctatg cttgcattat catccccaga aaatttaggt 1260aagcagataa atatatacaa agatgatgta tttaacctaa acgcaagcca taaggtagaa 1320ctccatagtc caatcagtaa ggaaacttat gaagttgatc tgggagagtt tgatactgtg 1380gtttcgaact taccttttgt tcaacaagag accctagctg aattaaatcc agaagctatt 1440agatttattg aagaattaaa tgaagcattc aacggtagaa gtgacttata tgcatacata 1500gcattaaaaa tagatgaaat acttccagaa aaagggacag ctggtttaat agtatctaat 1560tcttggcttg gaacagaatt tggagaaaga ttctttgatg agctaaagaa aagatatcat 1620attaaatata tattaacatc tggaaaaggt cgatggttcc aaaatgctga tgtagtaaca 1680aacatcattg tgttagaaaa aggaaatact tcccctgaca aaaaagttaa tttcataact 1740ctaaaaaaga ctcttcaaga aattgtcgtt gagggagaga aggagcaaca atttgagaat 1800gttgcaacaa tggttgcaaa aattcggagg gatatgcctt cagaattata tgagagtaac 1860agttattctt atggtgaaat tgagggcttg aataaacttg gtgtaattaa aaatgctctt 1920tttgcagatt gtagttggct ttttgatttt gaggataatt tagtcccatt aacggagttt 1980ttcaatgtta aacgtggaga acgtcggggg tggaatcctc tattctatcc gaagaatcac 2040aacattgaac ctgattatat agtacctgta atgaaaaaac tggatacatc ctcttatata 2100atgaatctta gcgcttcaat agaaggtttt agttgtagta gaaccattga agagcttgaa 2160gcactaaatc acagtggaac attagagtgg ataaagagtt ttgaaacagt taaaaatggt 2220agaggtgtgt tactaacaga agatttgcct agaaagaatg tacattggta tgaaatgcca 2280ctaaagaaga cctttgacat cggtttgtta attaatcctg atgagaggtt gtttttctca 2340aaagcacctc aaccagtatt ttttgaccaa agattaacag gtcttgttag gaagaatcct 2400caagatgact tagatatatt gacagcatta ttaaacagca ttgttggagt atactacatt 2460gaggcaattg gtttcggaag aggtctaggt gcattagact taaataaaaa taaagttgaa 2520gataaattta aaatgttgaa tccttcttta attagtgaac aagataaatt aataattttg 2580gaactgtata gagaactaga aaaacgtcag gttttgccat tacttcaaga agttcaacaa 2640agagaccgat atgattttga tatggctgtg ttaaaagctt ttggtttaga aaagcattat 2700gatagtataa aaaattctct tatgcagtta tttgcaatac gtaaatctgt gagataa 275748918PRTBacillus species 1310 48Met Lys Lys Val Gly Ala Thr Arg Asp Asn Glu Arg Ser Trp Ala Ile 1 5 10 15 Asp Leu Ile Ser Arg Ile Asn Ser Gly Ala Ile Val Cys Lys Glu Asp 20 25 30 Ser Met Ile Gln His Ala Gly Gly Glu Met Gly Leu Ser Thr Gly Ser 35 40 45 Gly Ser Leu Phe Pro Asp Val Leu Leu Phe Gly Asp Lys Gly Lys Thr 50 55 60 Arg Val Leu Gln Gly Trp Glu Leu Lys Tyr Pro Asp Thr Pro Ile Asp 65 70 75 80 Asp Arg Glu Leu Phe Ile Asn Ala Val Lys Lys Ala Glu Leu Leu Gly 85 90 95 Val Asn Ser Phe Leu Leu Trp Asn Val Ser Val Ala His Leu Tyr Val 100 105 110 Lys Asn Glu Glu Ser Gly Lys Tyr Glu Leu Leu Lys Lys Trp Asp Asp 115 120 125 Leu Lys His Ile Thr Lys Arg Ser Glu Val Ala Tyr Ser Met Ala Glu 130 135 140 Ile Asn Gln Val Leu Glu Ser Ile Leu Lys Asp Leu Glu Tyr Tyr Phe 145 150 155 160 Arg Asn Gly Thr Leu Arg Thr Glu Lys Ile Leu Asn Ser Ile Val Asn 165 170 175 Glu Gln Met Leu Ser Leu Ala Phe Asn Asn Ile Glu Asp Cys Ala Leu 180 185 190 Ser Leu Lys Asn Ala Ser Ala Lys Asp Ser Asp Phe Asn Asp Glu Ile 195 200 205 Ile Leu Trp Trp Glu Thr Glu Gly Leu Ser Tyr Gly Lys Lys Ala Asp 210 215 220 Arg Trp Ile Glu Leu Ser Arg Leu Ala Ile Ile Ser Leu Met Asn Lys 225 230 235 240 Leu Ile Phe Ala Asn Ile Leu Lys Lys Tyr Asn Ser His Ala Gln Ile 245 250 255 Ile Asp Gln Val Asn Asp Ser Leu Thr Val Glu Glu Cys Leu Asp Ile 260 265 270 Leu Asn Glu Ile Ser Glu Lys Cys Asp Phe Tyr Asn Ile Phe Glu Glu 275 280 285 Lys Pro Gly Glu Arg Tyr Ile Asp Val Ala Thr Leu Lys Val Leu Thr 290 295 300 Asn Phe Asn Asp Tyr Ile Met Asn Leu Asp Phe Asn Ser Tyr Ser Asp 305 310 315 320 Arg Leu Leu Glu Glu Leu Leu Asn Ile Val Val Thr Arg Ser Lys Arg 325 330 335 Lys Val Ala Gly Gln Phe Ser Thr Pro Lys Glu Leu Ala Met Ile Leu 340 345 350 Thr Ser Leu Thr Met Thr Asp Lys Ser Ser Arg Ile Ser Asp Pro Cys 355 360 365 Cys Gly Thr Gly Thr Ile Val Lys Ala Ala Tyr Asp Leu Lys Leu Val 370 375 380 Ser Gly Ile Asp Ser Ser Asp Ala Ile Asp Gln Ile Trp Ala Gly Asp 385 390 395 400 Lys Phe Arg Tyr Pro Leu Gln Phe Ala Met Leu Ala Leu Ser Ser Pro 405 410 415 Glu Asn Leu Gly Lys Gln Ile Asn Ile Tyr Lys Asp Asp Val Phe Asn 420 425 430 Leu Asn Ala Ser His Lys Val Glu Leu His Ser Pro Ile Ser Lys Glu 435 440 445 Thr Tyr Glu Val Asp Leu Gly Glu Phe Asp Thr Val Val Ser Asn Leu 450 455 460 Pro Phe Val Gln Gln Glu Thr Leu Ala Glu Leu Asn Pro Glu Ala Ile 465 470 475 480 Arg Phe Ile Glu Glu Leu Asn Glu Ala Phe Asn Gly Arg Ser Asp Leu 485 490 495 Tyr Ala Tyr Ile Ala Leu Lys Ile Asp Glu Ile Leu Pro Glu Lys Gly 500 505 510 Thr Ala Gly Leu Ile Val Ser Asn Ser Trp Leu Gly Thr Glu Phe Gly 515 520 525 Glu Arg Phe Phe Asp Glu Leu Lys Lys Arg Tyr His Ile Lys Tyr Ile 530 535 540 Leu Thr Ser Gly Lys Gly Arg Trp Phe Gln Asn Ala Asp Val Val Thr 545 550 555 560 Asn Ile Ile Val Leu Glu Lys Gly Asn Thr Ser Pro Asp Lys Lys Val 565 570 575 Asn Phe Ile Thr Leu Lys Lys Thr Leu Gln Glu Ile Val Val Glu Gly 580 585 590 Glu Lys Glu Gln Gln Phe Glu Asn Val Ala Thr Met Val Ala Lys Ile 595 600 605 Arg Arg Asp Met Pro Ser Glu Leu Tyr Glu Ser Asn Ser Tyr Ser Tyr 610 615 620 Gly Glu Ile Glu Gly Leu Asn Lys Leu Gly Val Ile Lys Asn Ala Leu 625 630 635 640 Phe Ala Asp Cys Ser Trp Leu Phe Asp Phe Glu Asp Asn Leu Val Pro 645 650 655 Leu Thr Glu Phe Phe Asn Val Lys Arg Gly Glu Arg Arg Gly Trp Asn 660 665 670 Pro Leu Phe Tyr Pro Lys Asn His Asn Ile Glu Pro Asp Tyr Ile Val 675 680 685 Pro Val Met Lys Lys Leu Asp Thr Ser Ser Tyr Ile Met Asn Leu Ser 690 695 700 Ala Ser Ile Glu Gly Phe Ser Cys Ser Arg Thr Ile Glu Glu Leu Glu 705 710 715 720 Ala Leu Asn His Ser Gly Thr Leu Glu Trp Ile Lys Ser Phe Glu Thr 725 730 735 Val Lys Asn Gly Arg Gly Val Leu Leu Thr Glu Asp Leu Pro Arg Lys 740 745 750 Asn Val His Trp Tyr Glu Met Pro Leu Lys Lys Thr Phe Asp Ile Gly 755 760 765 Leu Leu Ile Asn Pro Asp Glu Arg Leu Phe Phe Ser Lys Ala Pro Gln 770 775 780 Pro Val Phe Phe Asp Gln Arg Leu Thr Gly Leu Val Arg Lys Asn Pro 785 790 795 800 Gln Asp Asp Leu Asp Ile Leu Thr Ala Leu Leu Asn Ser Ile Val Gly 805 810 815 Val Tyr Tyr Ile Glu Ala Ile Gly Phe Gly Arg Gly Leu Gly Ala Leu 820 825 830 Asp Leu Asn Lys Asn Lys Val Glu Asp Lys Phe Lys Met Leu Asn Pro 835 840 845 Ser Leu Ile Ser Glu Gln Asp Lys Leu Ile Ile Leu Glu Leu Tyr Arg 850 855 860 Glu Leu Glu Lys Arg Gln Val Leu Pro Leu Leu Gln Glu Val Gln Gln 865 870 875 880 Arg Asp Arg Tyr Asp Phe Asp Met Ala Val Leu Lys Ala Phe Gly Leu 885 890 895 Glu Lys His Tyr Asp Ser Ile Lys Asn Ser Leu Met Gln Leu Phe Ala 900 905 910 Ile Arg Lys Ser Val Arg 915 491146DNABacillus species H 49gtgtcgaaat tatccgatgt ttttaaatat atatctttct atagaagtgc tggtcatcaa 60atagggcgaa aggttgggga tatgttagaa gtgctaacat atggagcttt acattacgat 120caaaatctga aaaaaagatt acatattgaa cctaacttat acggcttttc tgatgcaggg 180cataaagttg agtttttaat tacaaaagat gtaaatgaga atttacttaa gggagggagt 240gttactaatc tagaaaatta tataggtttt attgaatgta aaaaggtagg agtcgagcaa 300acagtctcaa cttcatttaa aaataaattt aaagattatg aaaataaaca gactaagaaa 360tatgatttaa aattagatag tatttttaat atcggttttt caagtcatgg tatgaataga 420cacaagttat ctgtatcttt tgcaaattgt gataataatt tattcattaa tgtaaaaaat 480gaaatcaata atgaaatcat ttttaatgaa caagtcaaag atcattatag acttatagtt 540gcacaatgta gtgataatag tatagatata ataggaaata gtcgaagttt aagagaattc 600aatttaccat taaataactg tcgtatatta gaaatatcta attttaattt acaggagaat 660agaatatctt tagttcttaa taattgttta gctggtccgc aaacaccaga aaaagcaaaa 720caggcttcat ttgttgcttt agatgttcgt aagaagagat ttggatcatt tgataaggtt 780gatgatccaa gctttaaaag tattttagtg ttaactgaat ttgcacactg ggaaagaaaa 840agcagaaata tgattagtgc ttgtattgat atcaatcttg tagtaccaga tagtatatta 900atcgaggctt tcgaagtatt taatcaatat tttgaaagaa atggcgcaac ggtatcaaat 960ttatatgatc taataacaaa agataacttt gaaaagaata aagagataca agatcttatt 1020atgagtatat taacagaata tgatggtaaa attttccaac aacttaagtc agatggtact 1080catattgaag aacttgtatc tctaaattac ttaaataata gtttatctat tatttctgaa 1140agataa 114650381PRTBacillus species H 50Val Ser Lys Leu Ser Asp Val Phe Lys Tyr Ile Ser Phe Tyr Arg Ser 1 5 10 15 Ala Gly His Gln Ile Gly Arg Lys Val Gly Asp Met Leu Glu Val Leu 20 25 30 Thr Tyr Gly Ala Leu His Tyr Asp Gln Asn Leu Lys Lys Arg Leu His 35 40 45 Ile Glu Pro Asn Leu Tyr Gly Phe Ser Asp Ala Gly His Lys Val Glu 50 55 60 Phe Leu Ile Thr Lys Asp Val Asn Glu Asn Leu Leu Lys Gly Gly Ser 65 70 75 80 Val Thr Asn Leu Glu Asn Tyr Ile Gly Phe Ile Glu Cys Lys Lys Val 85 90 95 Gly Val Glu Gln Thr Val Ser Thr Ser Phe Lys Asn Lys Phe Lys Asp 100 105 110 Tyr Glu Asn Lys Gln Thr Lys Lys Tyr Asp Leu Lys Leu Asp Ser Ile 115 120 125 Phe Asn Ile Gly Phe Ser Ser His Gly Met Asn Arg His Lys Leu Ser 130 135 140 Val Ser Phe Ala Asn Cys Asp Asn Asn Leu Phe Ile Asn Val Lys Asn 145 150 155 160 Glu Ile Asn Asn Glu Ile Ile Phe Asn Glu Gln Val Lys Asp His Tyr 165 170 175 Arg Leu Ile Val Ala Gln Cys Ser Asp Asn Ser Ile Asp Ile Ile Gly 180 185 190 Asn Ser Arg Ser Leu Arg Glu Phe Asn Leu Pro Leu Asn Asn Cys Arg 195 200 205 Ile Leu Glu Ile Ser Asn Phe Asn Leu Gln Glu Asn Arg Ile Ser Leu 210 215 220 Val Leu Asn Asn Cys Leu Ala Gly Pro Gln Thr Pro Glu Lys Ala Lys 225 230 235 240 Gln Ala Ser Phe Val Ala Leu Asp Val Arg Lys Lys Arg Phe Gly Ser 245 250 255 Phe Asp Lys Val Asp Asp Pro Ser Phe Lys Ser Ile Leu Val Leu Thr 260 265 270 Glu Phe Ala His Trp Glu Arg Lys Ser Arg Asn Met Ile Ser Ala Cys 275 280 285 Ile Asp Ile Asn Leu Val Val Pro Asp Ser Ile Leu Ile Glu Ala Phe 290 295 300 Glu Val Phe Asn Gln Tyr Phe Glu Arg Asn Gly Ala Thr Val Ser Asn 305 310 315 320 Leu Tyr Asp Leu Ile Thr Lys Asp Asn Phe Glu Lys Asn Lys Glu Ile 325 330 335 Gln Asp Leu Ile Met Ser Ile Leu Thr Glu Tyr Asp Gly Lys Ile Phe 340 345 350 Gln Gln Leu Lys Ser Asp Gly Thr His Ile Glu Glu Leu Val Ser Leu 355 360 365 Asn Tyr Leu Asn Asn Ser Leu Ser Ile Ile Ser Glu Arg 370 375 380 511490DNABacillus species M 51gcgataccac aatgagtcat gatttatatg ctgcttgggc agctacagaa attactaata 60ttttgcaaac aaatccccgt tttttagtga gtgacggtat atctagaaat tttactgttt 120atgctagtaa agaaggaaga accaagtggc ctattgctga tggtgttatt cttgttgaag 180aaaatggccg agtggtttat gagatagcaa tagagttcaa acgacgaaac gagggagtgc 240acggtgtact tactgccctt ggccaggctc atgcctattt acataaagga tatcgaggat 300cgataattgt tattccagaa gcctacgata cccataataa tccatcaggg catttaaaag 360aaataattga atatactagc gatcaagtcc ctattggagt attcagttat aaagatcctg 420atgtcactaa gacttctccg tttaatggta aaattacatg tattagacat ttgaatttga 480atactgggct aggttctgtt gttaggtcct ctagccctca aaattttgtt aaaactcaat 540gggctcattt gagggagggt agtagtgatc ctgatgcatt ttttcgatac ttacaaacat 600ctaagcagct agcaattgat agcttaattg agccatcagt aaatttcccc ccgagtctag 660ttcaggctat tcaagatata caaccaggtg caaacccact aaaatacttg tctaattcaa 720taggcaatga tctacatgat atagtatgga gaaatttttg gtttaattac attcttaccg 780acgaggccat acctatttgg aataattctg aaggtaacta tgtaattaat gattcatcta 840caaaaatagt taaaccagac gagagcggga acaagatgtt ttttgctggt cgttcagatt 900caataaaaaa tcgattagtg aacgatctga atatgggaaa tatctcagag tctgaagcat 960ggaaaaaata cgctctaaag attcgtgaaa gagcacatag ctatagggag gatatagact 1020ctggtttaga tcatattgga ttacttgaaa gtgatggtaa accttcagag cttggatacc 1080gctttgttga tgcatgtgaa agaactagaa atagtaattc aggcagtcct aaggctcttt 1140taggagctgc aattcttaaa aatggaaatt taggggcgtt tttgcattat atataccgtc 1200tttcagaaga aaaatttaat gcagacccct tggcctttac aaaacaaaac aattcatcag 1260gacgcttaca atttctgcat aaggaatatt tgcaatggtt agagaatgaa ttggctacta 1320atctaaaagt tatgaggaaa gttagtatta ggggaggagc aagtagacag cctttccaag 1380gtgaacttgc tattctgagg aattatgaat ttgtaggaaa ctttcgggta ggtacaggat 1440taaaaataaa ctggcccaaa atccaaaatg cttatgaagt agagatataa 149052496PRTBacillus species M 52Gly Asp Thr Thr Met Ser His Asp Leu Tyr Ala Ala Trp Ala Ala Thr 1 5 10 15 Glu Ile Thr Asn Ile Leu Gln Thr Asn Pro Arg Phe Leu Val Ser Asp 20 25 30 Gly Ile Ser Arg Asn Phe Thr Val Tyr Ala Ser Lys Glu Gly Arg Thr 35 40 45 Lys Trp Pro Ile Ala Asp Gly Val Ile Leu Val Glu Glu Asn Gly Arg 50 55 60 Val Val Tyr Glu Ile Ala Ile Glu Phe Lys Arg Arg Asn Glu Gly Val 65 70 75 80 His Gly Val Leu Thr Ala Leu Gly Gln Ala His Ala Tyr Leu His Lys 85 90 95 Gly Tyr Arg Gly Ser Ile Ile Val Ile Pro Glu Ala Tyr Asp Thr His 100 105 110 Asn Asn Pro Ser Gly His Leu Lys Glu Ile Ile Glu Tyr Thr Ser Asp 115 120 125 Gln Val Pro Ile Gly Val Phe Ser Tyr Lys Asp Pro Asp Val Thr Lys 130 135 140 Thr Ser Pro Phe Asn Gly Lys Ile Thr Cys Ile Arg His Leu Asn Leu 145 150 155 160 Asn Thr Gly Leu Gly Ser Val Val Arg Ser Ser Ser Pro Gln Asn Phe 165 170 175 Val Lys Thr Gln Trp Ala His Leu Arg Glu Gly Ser Ser Asp Pro Asp 180 185 190 Ala Phe Phe Arg Tyr Leu Gln Thr Ser Lys Gln Leu Ala Ile Asp Ser 195 200 205 Leu Ile Glu Pro Ser Val Asn Phe Pro Pro Ser Leu Val Gln Ala Ile 210 215 220 Gln Asp Ile Gln Pro Gly Ala Asn Pro Leu Lys Tyr Leu Ser Asn Ser 225 230 235 240 Ile Gly Asn

Asp Leu His Asp Ile Val Trp Arg Asn Phe Trp Phe Asn 245 250 255 Tyr Ile Leu Thr Asp Glu Ala Ile Pro Ile Trp Asn Asn Ser Glu Gly 260 265 270 Asn Tyr Val Ile Asn Asp Ser Ser Thr Lys Ile Val Lys Pro Asp Glu 275 280 285 Ser Gly Asn Lys Met Phe Phe Ala Gly Arg Ser Asp Ser Ile Lys Asn 290 295 300 Arg Leu Val Asn Asp Leu Asn Met Gly Asn Ile Ser Glu Ser Glu Ala 305 310 315 320 Trp Lys Lys Tyr Ala Leu Lys Ile Arg Glu Arg Ala His Ser Tyr Arg 325 330 335 Glu Asp Ile Asp Ser Gly Leu Asp His Ile Gly Leu Leu Glu Ser Asp 340 345 350 Gly Lys Pro Ser Glu Leu Gly Tyr Arg Phe Val Asp Ala Cys Glu Arg 355 360 365 Thr Arg Asn Ser Asn Ser Gly Ser Pro Lys Ala Leu Leu Gly Ala Ala 370 375 380 Ile Leu Lys Asn Gly Asn Leu Gly Ala Phe Leu His Tyr Ile Tyr Arg 385 390 395 400 Leu Ser Glu Glu Lys Phe Asn Ala Asp Pro Leu Ala Phe Thr Lys Gln 405 410 415 Asn Asn Ser Ser Gly Arg Leu Gln Phe Leu His Lys Glu Tyr Leu Gln 420 425 430 Trp Leu Glu Asn Glu Leu Ala Thr Asn Leu Lys Val Met Arg Lys Val 435 440 445 Ser Ile Arg Gly Gly Ala Ser Arg Gln Pro Phe Gln Gly Glu Leu Ala 450 455 460 Ile Leu Arg Asn Tyr Glu Phe Val Gly Asn Phe Arg Val Gly Thr Gly 465 470 475 480 Leu Lys Ile Asn Trp Pro Lys Ile Gln Asn Ala Tyr Glu Val Glu Ile 485 490 495 531314DNABacillus stearothermophilus CPW193 53atgagtgata aggttaattt ttcttctaac aatattgacc aaaactatag tattgagata 60tccgaatttg agtttggaac tggaagaatt gccgatataa tcagggcact caaagattat 120tatggcgttg aatctttgga aaatttaaca catagtcaaa agcttgatgg tctgtgtaaa 180gctcttcagt ttactccatc tcaactggat cgtttaatag ctcaaaattc tcctgtactt 240cgtaccatta agggccatgc atttgagaga gtttttgatg aaattcttaa aatgaatgga 300tatgaggtaa ctgaagtcgg aggagacagt ggagttgata gaattgtaaa taataaaacc 360cttcagctaa aaactcctaa taaggctgga acaaaggaaa atgtcgtaga atacaaaaca 420cataaaactc atggtgctaa atctgagagg gagtctttag attactacta tagtaaagaa 480gactttgctg attatttagt tggtcttgtt tcatatgagc cttttaacat tctctttata 540cctcgggagg aattgccgac aatttccaaa gattcatcaa agattaagag tccatttaag 600gtagaatggg actcaaaccc aggtttaaac tcctttaaat ctattggtat agacaatatt 660gttatatcag aaaaaattta taaacctgca catgggaatg aacttttacc tttatcatca 720aggaaactcc aactaaaaag cgagataata attgatgtga ttttaaatga aagtaatttc 780cgtatatggg atatgaatat gagaggattt gctagagaaa tggcttttgt cgagtatcta 840tcatcttttg ggattagagt atttaaccct gcaaattgca gaaaagaaag ggcagataag 900gctgacatag cattaaaatc tgcccaaaat ggcaactttt cttttctaca aattaaaggt 960attacattag atttagataa tttccggggg agagaatcaa ttgttgatgt tgagacacag 1020ctttcacgtg gacgggtaaa tgatcatcca acacaaagta ggctctatct tgaaactgat 1080tttgattatt taattgtctg tatagaccca tgttattcaa aactttactc taaagaaatt 1140ggcaagccta attgttttga ctgggagttt tatgctatcc ctaacaatgt tttagaacgt 1200catccaaaat acactcgtcg aataaaatca catcaaaaga taaaatatgt tgaattacag 1260agatatagaa tagatgatac ttggattaat ttgtgggaaa aaggagctaa ctaa 131454437PRTBacillus stearothermophilus CPW193 54Met Ser Asp Lys Val Asn Phe Ser Ser Asn Asn Ile Asp Gln Asn Tyr 1 5 10 15 Ser Ile Glu Ile Ser Glu Phe Glu Phe Gly Thr Gly Arg Ile Ala Asp 20 25 30 Ile Ile Arg Ala Leu Lys Asp Tyr Tyr Gly Val Glu Ser Leu Glu Asn 35 40 45 Leu Thr His Ser Gln Lys Leu Asp Gly Leu Cys Lys Ala Leu Gln Phe 50 55 60 Thr Pro Ser Gln Leu Asp Arg Leu Ile Ala Gln Asn Ser Pro Val Leu 65 70 75 80 Arg Thr Ile Lys Gly His Ala Phe Glu Arg Val Phe Asp Glu Ile Leu 85 90 95 Lys Met Asn Gly Tyr Glu Val Thr Glu Val Gly Gly Asp Ser Gly Val 100 105 110 Asp Arg Ile Val Asn Asn Lys Thr Leu Gln Leu Lys Thr Pro Asn Lys 115 120 125 Ala Gly Thr Lys Glu Asn Val Val Glu Tyr Lys Thr His Lys Thr His 130 135 140 Gly Ala Lys Ser Glu Arg Glu Ser Leu Asp Tyr Tyr Tyr Ser Lys Glu 145 150 155 160 Asp Phe Ala Asp Tyr Leu Val Gly Leu Val Ser Tyr Glu Pro Phe Asn 165 170 175 Ile Leu Phe Ile Pro Arg Glu Glu Leu Pro Thr Ile Ser Lys Asp Ser 180 185 190 Ser Lys Ile Lys Ser Pro Phe Lys Val Glu Trp Asp Ser Asn Pro Gly 195 200 205 Leu Asn Ser Phe Lys Ser Ile Gly Ile Asp Asn Ile Val Ile Ser Glu 210 215 220 Lys Ile Tyr Lys Pro Ala His Gly Asn Glu Leu Leu Pro Leu Ser Ser 225 230 235 240 Arg Lys Leu Gln Leu Lys Ser Glu Ile Ile Ile Asp Val Ile Leu Asn 245 250 255 Glu Ser Asn Phe Arg Ile Trp Asp Met Asn Met Arg Gly Phe Ala Arg 260 265 270 Glu Met Ala Phe Val Glu Tyr Leu Ser Ser Phe Gly Ile Arg Val Phe 275 280 285 Asn Pro Ala Asn Cys Arg Lys Glu Arg Ala Asp Lys Ala Asp Ile Ala 290 295 300 Leu Lys Ser Ala Gln Asn Gly Asn Phe Ser Phe Leu Gln Ile Lys Gly 305 310 315 320 Ile Thr Leu Asp Leu Asp Asn Phe Arg Gly Arg Glu Ser Ile Val Asp 325 330 335 Val Glu Thr Gln Leu Ser Arg Gly Arg Val Asn Asp His Pro Thr Gln 340 345 350 Ser Arg Leu Tyr Leu Glu Thr Asp Phe Asp Tyr Leu Ile Val Cys Ile 355 360 365 Asp Pro Cys Tyr Ser Lys Leu Tyr Ser Lys Glu Ile Gly Lys Pro Asn 370 375 380 Cys Phe Asp Trp Glu Phe Tyr Ala Ile Pro Asn Asn Val Leu Glu Arg 385 390 395 400 His Pro Lys Tyr Thr Arg Arg Ile Lys Ser His Gln Lys Ile Lys Tyr 405 410 415 Val Glu Leu Gln Arg Tyr Arg Ile Asp Asp Thr Trp Ile Asn Leu Trp 420 425 430 Glu Lys Gly Ala Asn 435 551467DNABacillus stearothermophilus D70 55atgacagaat atgacttaca tttatatgct gatagtttcc atgaaggaca ttggtgttgt 60gaaaacttag caaaaattgc acaatcagat ggaggaaaac accaaattga ttatcttcaa 120gggtttatac ctagacattc tttaatattc agcgatttaa taattaatat tactgtattc 180ggttcttaca aatcatggaa acatttacct aaacaaatta aagacctttt attctggggg 240aaacctgatt ttatagcata tgacccaaaa aatgataaaa tcttgtttgc agttgaagaa 300acgggagcag ttccaacagg taatcaggct ttacaaagat gcgaaagaat ctacggaagc 360gcaagaaaac aaataccttt ctggtattta ttaagtgagt tcggtcaaca taaggatggt 420ggaacccgtc gtgattccat ttggcctact ataatgggat taaagttaac acagttagta 480aaaacacctt cgattatatt acactattca gatatcaata atcccgaaga ttataattct 540ggtaatggtc taaaattttt gtttaaatct ctactacaaa ttattatcaa ttactgcact 600cttaaaaatc ctttaaaagg tatgttggaa ttactgtcta ttcaatacga aaatatgtta 660gaattcatta aatcccaatg gaaagagcag attgacttct taccaggaga agaaatttta 720aatacaaaaa caaaagaact agctcgcatg tacgcatctt tagcaatagg acaaacagtg 780aagattccag aagaattgtt taattggcca agaacagaca aagttaattt caagagtcca 840cagggattaa ttaagtatga tgagttatgt tatcaattag aaaaagctgt aggaagcaaa 900aaagcttatt gtttatctaa taatgctgga gctaaaccac aaaaattaga atctttaaaa 960gaatggataa atagtcaaaa gaaattattt gataaagctc caaaactaac acctccagca 1020gaatttaata tgaagttaga tgcttttcct gttacatcaa acaataatta ttatgttact 1080acttctaaaa atattttata tctattcgat tattggaaag acttacgcat tgctatagaa 1140accgcttttc ctagattaaa aggtaagttg ccaactgata ttgatgagaa acctgctcta 1200atctatatct gtaatagcgt taagccaggt cgattatttg gagatccttt tactggtcaa 1260ctttctgcat tttctactat ttttggaaaa aaaaatattg acatgccacg aatagtggta 1320gcttattatc cacatcaaat ttatagtcaa gctcttccaa agaataacaa atctaataaa 1380ggaataactt taaaaaagga gttaaccgat ttcttaattt ttcatggggg agtagttgtt 1440aaattaaatg aagggaaggc atattaa 146756488PRTBacillus stearothermophilus D70 56Met Thr Glu Tyr Asp Leu His Leu Tyr Ala Asp Ser Phe His Glu Gly 1 5 10 15 His Trp Cys Cys Glu Asn Leu Ala Lys Ile Ala Gln Ser Asp Gly Gly 20 25 30 Lys His Gln Ile Asp Tyr Leu Gln Gly Phe Ile Pro Arg His Ser Leu 35 40 45 Ile Phe Ser Asp Leu Ile Ile Asn Ile Thr Val Phe Gly Ser Tyr Lys 50 55 60 Ser Trp Lys His Leu Pro Lys Gln Ile Lys Asp Leu Leu Phe Trp Gly 65 70 75 80 Lys Pro Asp Phe Ile Ala Tyr Asp Pro Lys Asn Asp Lys Ile Leu Phe 85 90 95 Ala Val Glu Glu Thr Gly Ala Val Pro Thr Gly Asn Gln Ala Leu Gln 100 105 110 Arg Cys Glu Arg Ile Tyr Gly Ser Ala Arg Lys Gln Ile Pro Phe Trp 115 120 125 Tyr Leu Leu Ser Glu Phe Gly Gln His Lys Asp Gly Gly Thr Arg Arg 130 135 140 Asp Ser Ile Trp Pro Thr Ile Met Gly Leu Lys Leu Thr Gln Leu Val 145 150 155 160 Lys Thr Pro Ser Ile Ile Leu His Tyr Ser Asp Ile Asn Asn Pro Glu 165 170 175 Asp Tyr Asn Ser Gly Asn Gly Leu Lys Phe Leu Phe Lys Ser Leu Leu 180 185 190 Gln Ile Ile Ile Asn Tyr Cys Thr Leu Lys Asn Pro Leu Lys Gly Met 195 200 205 Leu Glu Leu Leu Ser Ile Gln Tyr Glu Asn Met Leu Glu Phe Ile Lys 210 215 220 Ser Gln Trp Lys Glu Gln Ile Asp Phe Leu Pro Gly Glu Glu Ile Leu 225 230 235 240 Asn Thr Lys Thr Lys Glu Leu Ala Arg Met Tyr Ala Ser Leu Ala Ile 245 250 255 Gly Gln Thr Val Lys Ile Pro Glu Glu Leu Phe Asn Trp Pro Arg Thr 260 265 270 Asp Lys Val Asn Phe Lys Ser Pro Gln Gly Leu Ile Lys Tyr Asp Glu 275 280 285 Leu Cys Tyr Gln Leu Glu Lys Ala Val Gly Ser Lys Lys Ala Tyr Cys 290 295 300 Leu Ser Asn Asn Ala Gly Ala Lys Pro Gln Lys Leu Glu Ser Leu Lys 305 310 315 320 Glu Trp Ile Asn Ser Gln Lys Lys Leu Phe Asp Lys Ala Pro Lys Leu 325 330 335 Thr Pro Pro Ala Glu Phe Asn Met Lys Leu Asp Ala Phe Pro Val Thr 340 345 350 Ser Asn Asn Asn Tyr Tyr Val Thr Thr Ser Lys Asn Ile Leu Tyr Leu 355 360 365 Phe Asp Tyr Trp Lys Asp Leu Arg Ile Ala Ile Glu Thr Ala Phe Pro 370 375 380 Arg Leu Lys Gly Lys Leu Pro Thr Asp Ile Asp Glu Lys Pro Ala Leu 385 390 395 400 Ile Tyr Ile Cys Asn Ser Val Lys Pro Gly Arg Leu Phe Gly Asp Pro 405 410 415 Phe Thr Gly Gln Leu Ser Ala Phe Ser Thr Ile Phe Gly Lys Lys Asn 420 425 430 Ile Asp Met Pro Arg Ile Val Val Ala Tyr Tyr Pro His Gln Ile Tyr 435 440 445 Ser Gln Ala Leu Pro Lys Asn Asn Lys Ser Asn Lys Gly Ile Thr Leu 450 455 460 Lys Lys Glu Leu Thr Asp Phe Leu Ile Phe His Gly Gly Val Val Val 465 470 475 480 Lys Leu Asn Glu Gly Lys Ala Tyr 485 57654DNABacillus stearothermophilus D70 57atgactgatt atagatattc atttgaacta agtgaagaga ttgcaagatg ggcattcgaa 60ataaaaacaa aaaatacaga ttggtttgta gctttttcta atccaacggc tggtccttgg 120aaaagagtaa tggcaataga taaggcttct aatagagaag gagaagtaca tagatttgga 180agagaagatg agcgtcctga tattattcta gttaatgata atatatcatt aatattgata 240ttggaggcca aagaaaaatt gaaccagtta atcagtaaat cgcaagtaga taaatcagtt 300gatgtgtttt taactctctc cagtattctg aaagaaaagt ctgataataa ttattgggga 360gatagaacaa agtacataaa tgtgttagga attctatggg gaagcgaaca agaaacttcc 420caaaaagata ttgataatgc gtttagagtt tatagagatt ccctagttaa aaatttaaaa 480gaaatcaacc ctacacctac caatatttgt acggatattt tagtaggtgt agagtctatc 540aagaacaaaa aagaagaaat atctattaaa attcatgttt ctaatatata tgcggaaata 600tatcctaaat ttactggaaa acatcttctg gaaaagttag ctgttctgaa ttag 65458217PRTBacillus stearothermophilus D70 58Met Thr Asp Tyr Arg Tyr Ser Phe Glu Leu Ser Glu Glu Ile Ala Arg 1 5 10 15 Trp Ala Phe Glu Ile Lys Thr Lys Asn Thr Asp Trp Phe Val Ala Phe 20 25 30 Ser Asn Pro Thr Ala Gly Pro Trp Lys Arg Val Met Ala Ile Asp Lys 35 40 45 Ala Ser Asn Arg Glu Gly Glu Val His Arg Phe Gly Arg Glu Asp Glu 50 55 60 Arg Pro Asp Ile Ile Leu Val Asn Asp Asn Ile Ser Leu Ile Leu Ile 65 70 75 80 Leu Glu Ala Lys Glu Lys Leu Asn Gln Leu Ile Ser Lys Ser Gln Val 85 90 95 Asp Lys Ser Val Asp Val Phe Leu Thr Leu Ser Ser Ile Leu Lys Glu 100 105 110 Lys Ser Asp Asn Asn Tyr Trp Gly Asp Arg Thr Lys Tyr Ile Asn Val 115 120 125 Leu Gly Ile Leu Trp Gly Ser Glu Gln Glu Thr Ser Gln Lys Asp Ile 130 135 140 Asp Asn Ala Phe Arg Val Tyr Arg Asp Ser Leu Val Lys Asn Leu Lys 145 150 155 160 Glu Ile Asn Pro Thr Pro Thr Asn Ile Cys Thr Asp Ile Leu Val Gly 165 170 175 Val Glu Ser Ile Lys Asn Lys Lys Glu Glu Ile Ser Ile Lys Ile His 180 185 190 Val Ser Asn Ile Tyr Ala Glu Ile Tyr Pro Lys Phe Thr Gly Lys His 195 200 205 Leu Leu Glu Lys Leu Ala Val Leu Asn 210 215 591800DNABacillus stearothermophilus 59atgagaaata ttcgtatcta ttctgaagta aaggaacaag ggatattttt taaagaagta 60attcaatctg ttttagaaaa agctaacgtt gaagtagttt tagtaaattc agcgatgttg 120gattatagtg acgtatctgt catttctttg atacgtaatc aaaagaagtt tgatttgtta 180gtatcggaag ttagggataa acgcgaaatt cctattgtta tggttgaatt ctcaacggca 240gtaactacag atgatcatga acttcaacga gcagatgcaa tgttttgggc atacaagtat 300aaaataccat atttaaaaat atcacctatg gagaaaaaat cacagacagc agatgataaa 360tttggtggcg gaaggctttt aagtgtaaat gaccagatta ttcacatgta tagaacggat 420ggtgtaatgt atcatattga atgggaatca atggataatt ctgcatatgt gaagaatgcg 480gaactatatc cttcttgtcc tgattgtgca ccggaattag cgtctctatt tagatgtctt 540ttggaaacaa ttgagaaatg tgagaatata gaagattatt ataggatttt gttagataag 600ttaggtaaac aaaaagtggc cgtaaaatgg gggaatttcc gtgaagaaaa aacacttgaa 660cagtggaagc atgaaaaatt tgatttattg gagcgcttta gtaaaagttc ttcacgtatg 720gagtatgata aagacaaaaa agagttaaaa attaaagtta atcgatatgg ccacgcgatg 780gatccggaac gaggcattct ggccttttgg aaactagttc ttggagacga atggaagatt 840gttgcggaat ttcagttgca acgcaaaaca ctcaagggga gacaatctta tcaatcgctt 900tttgatgaag tttctcaaga agaaaaatta atgaacatag catctgaaat tataaagaat 960ggaaatgtta tttctcctga taaagcaata gaaattcaca aattagctac ttcttccaca 1020atgattagta caattgattt gggaactcca gaacgtaaat atattacaga tgactcttta 1080aaagggtatt tgcaacatgg attaattacg aatatttaca aaaatttgct ttattatgta 1140gatgaaattc gatttacaga tttacaaaga aaaacaatcg cttctttgac atggaataag 1200gagattgtaa atgattatta taaatcatta atggatcagt tgttagataa gaacttaaga 1260gtattaccgt tgacatcaat caagaatatt tctgaagact tgattacatg gtctagtaaa 1320gaaattctta taaatcttgg atataagatt ttagcagcta gttatccaga ggctcaagga 1380gatcgttgta ttttagttgg tcctactggc aagaagactg aaagaaagtt tattgactta 1440attgctattt ctcctaaaag taaaggggtt atattattag aatgtaagga taagttgagt 1500aaatcgaaag atgattgtga aaaaatgaat gatcttctta atcataacta tgataaagtt 1560acgaaattaa taaatgtatt gaatattaac aattataatt ataataatat tatatataca 1620ggagtagcag gtctaattgg aaggaaaaat gttgacaatc ttcctgtaga tttcgtgatt 1680aaatttaaat atgatgctaa aaacctcaaa ctaaattggg aaataaatag tgatatttta 1740ggtaaacata gtggcagttt tagtatggaa gatgtagcag tagtgcgaaa acgatcataa 180060598PRTBacillus stearothermophilus 60Met Arg Asn Ile Arg Ile Tyr Ser Glu Val Lys Glu Gln Gly Ile Phe 1 5 10 15 Phe Lys Glu Val Ile Gln Ser Val Leu Glu Lys Ala Asn Val Glu Val 20 25 30 Val Leu Val Asn Ser Ala Met Leu Asp Tyr Ser Asp Val Ser Val Ile 35 40 45 Ser Leu Ile Arg

Asn Gln Lys Lys Phe Asp Leu Leu Val Ser Glu Val 50 55 60 Arg Asp Lys Arg Glu Ile Pro Ile Val Met Val Glu Phe Ser Thr Ala 65 70 75 80 Val Thr Thr Asp Asp His Glu Leu Gln Arg Ala Asp Ala Met Phe Trp 85 90 95 Ala Tyr Lys Tyr Lys Ile Pro Tyr Leu Lys Ile Ser Pro Met Glu Lys 100 105 110 Lys Ser Gln Thr Ala Asp Asp Lys Phe Gly Gly Gly Arg Leu Leu Ser 115 120 125 Val Asn Asp Gln Ile Ile His Met Tyr Arg Thr Asp Gly Val Met Tyr 130 135 140 His Ile Glu Trp Glu Ser Met Asp Asn Ser Ala Tyr Val Lys Asn Ala 145 150 155 160 Glu Leu Tyr Pro Ser Cys Pro Asp Cys Ala Pro Glu Leu Ala Ser Leu 165 170 175 Phe Arg Cys Leu Leu Glu Thr Ile Glu Lys Cys Glu Asn Ile Glu Asp 180 185 190 Tyr Tyr Arg Ile Leu Leu Asp Lys Leu Gly Lys Gln Lys Val Ala Val 195 200 205 Lys Trp Gly Asn Phe Arg Glu Glu Lys Thr Leu Glu Gln Trp Lys His 210 215 220 Glu Lys Phe Asp Leu Leu Arg Phe Ser Lys Ser Ser Ser Arg Met Glu 225 230 235 240 Tyr Asp Lys Asp Lys Lys Glu Leu Lys Ile Lys Val Asn Arg Tyr Gly 245 250 255 His Ala Met Asp Pro Glu Arg Gly Ile Leu Ala Phe Trp Lys Leu Val 260 265 270 Leu Gly Asp Glu Trp Lys Ile Val Ala Glu Phe Gln Leu Gln Arg Lys 275 280 285 Thr Leu Lys Gly Arg Gln Ser Tyr Gln Ser Leu Phe Asp Glu Val Ser 290 295 300 Gln Glu Glu Lys Leu Met Asn Ile Ala Ser Glu Ile Ile Lys Asn Gly 305 310 315 320 Asn Val Ile Ser Pro Asp Lys Ala Ile Glu Ile His Lys Leu Ala Thr 325 330 335 Ser Ser Thr Met Ile Ser Thr Ile Asp Leu Gly Thr Pro Glu Arg Lys 340 345 350 Tyr Ile Thr Asp Asp Ser Leu Lys Gly Tyr Leu Gln His Gly Leu Ile 355 360 365 Thr Asn Ile Tyr Lys Asn Leu Leu Tyr Tyr Val Asp Glu Ile Arg Phe 370 375 380 Thr Asp Leu Gln Arg Lys Thr Ile Ala Ser Leu Thr Trp Asn Lys Glu 385 390 395 400 Ile Val Asn Asp Tyr Tyr Lys Ser Leu Met Asp Gln Leu Leu Asp Lys 405 410 415 Asn Leu Arg Val Leu Pro Leu Thr Ser Ile Lys Asn Ile Ser Glu Asp 420 425 430 Leu Ile Thr Trp Ser Ser Lys Glu Ile Leu Ile Asn Leu Gly Tyr Lys 435 440 445 Ile Leu Ala Ala Ser Tyr Pro Glu Ala Gln Gly Asp Arg Cys Ile Leu 450 455 460 Val Gly Pro Thr Gly Lys Lys Thr Glu Arg Lys Phe Ile Asp Leu Ile 465 470 475 480 Ala Ile Ser Pro Lys Ser Lys Gly Val Ile Leu Leu Glu Cys Lys Asp 485 490 495 Lys Leu Ser Lys Ser Lys Asp Asp Cys Glu Lys Met Asn Asp Leu Leu 500 505 510 Asn His Asn Tyr Asp Lys Val Thr Lys Leu Ile Asn Val Leu Asn Ile 515 520 525 Asn Asn Tyr Asn Tyr Asn Asn Ile Ile Tyr Thr Gly Val Ala Gly Leu 530 535 540 Ile Gly Arg Lys Asn Val Asp Asn Leu Pro Val Asp Phe Val Ile Lys 545 550 555 560 Phe Lys Tyr Asp Ala Lys Asn Leu Lys Leu Asn Trp Glu Ile Asn Ser 565 570 575 Asp Ile Leu Gly Lys His Ser Gly Ser Phe Ser Met Glu Asp Val Ala 580 585 590 Val Val Arg Lys Arg Ser 595 61867DNABacillus stearothermophilus ET 61atgataaaaa actttagaga ctatcaacga gtagcagcta aatacataac atttattgaa 60tcagaatttt accctgacta tctagataat gctcgttttt tatatgggga agtattaaat 120aaattctatg aattagtaaa tagctcttct agctctatag agttgttgga aaatatttca 180aaaacaaaag atcctgtccg aactcaactg ttacggattt ttagaaagta tgtttcacct 240gatacttcag ttgaaatgtt aaaaagaaaa cagagaattc ccgatattat taaagagttt 300ggaacaagat ttcgggacat taaaatagta agacaaaaaa ttgctactcg caatcatcct 360gatgaaacca taatggctct cctttacgaa tacaaagatc gaggaaaaaa aggatatgaa 420ttgactgatg cattttttac atggtttgaa cagaagtttc ctaattacga aatcattgga 480ccaagagggg ctggtaaaga tatactacta aatgaagtat taccaggatt tccatcaaaa 540atccctgcag atttcctaat atatagaaga tctgataaaa cccctatagt agttggattt 600gcaagatatg attcagatag aggaggtgct caagaagatg atagaacagg tggcaataga 660gataaaatca ccgaaataaa aaagtatgct gcggagcata acattccttt aaaaatttta 720tttttaaatg acggtcctgg attactttta ggttctatgt ggaatgatta ctccgcatta 780gaagattatg gtgaagggtg cgttatggtt tgtacattaa aaatgttgga ggagcgtttt 840acaatcgatt ggcttgaaaa tttataa 86762288PRTBacillus stearothermophilus ET 62Met Ile Lys Asn Phe Arg Asp Tyr Gln Arg Val Ala Ala Lys Tyr Ile 1 5 10 15 Thr Phe Ile Glu Ser Glu Phe Tyr Pro Asp Tyr Leu Asp Asn Ala Arg 20 25 30 Phe Leu Tyr Gly Glu Val Leu Asn Lys Phe Tyr Glu Leu Val Asn Ser 35 40 45 Ser Ser Ser Ser Ile Glu Leu Leu Glu Asn Ile Ser Lys Thr Lys Asp 50 55 60 Pro Val Arg Thr Gln Leu Leu Arg Ile Phe Arg Lys Tyr Val Ser Pro 65 70 75 80 Asp Thr Ser Val Glu Met Leu Lys Arg Lys Gln Arg Ile Pro Asp Ile 85 90 95 Ile Lys Glu Phe Gly Thr Arg Phe Arg Asp Ile Lys Ile Val Arg Gln 100 105 110 Lys Ile Ala Thr Arg Asn His Pro Asp Glu Thr Ile Met Ala Leu Leu 115 120 125 Tyr Glu Tyr Lys Asp Arg Gly Lys Lys Gly Tyr Glu Leu Thr Asp Ala 130 135 140 Phe Phe Thr Trp Phe Glu Gln Lys Phe Pro Asn Tyr Glu Ile Ile Gly 145 150 155 160 Pro Arg Gly Ala Gly Lys Asp Ile Leu Leu Asn Glu Val Leu Pro Gly 165 170 175 Phe Pro Ser Lys Ile Pro Ala Asp Phe Leu Ile Tyr Arg Arg Ser Asp 180 185 190 Lys Thr Pro Ile Val Val Gly Phe Ala Arg Tyr Asp Ser Asp Arg Gly 195 200 205 Gly Ala Gln Glu Asp Asp Arg Thr Gly Gly Asn Arg Asp Lys Ile Thr 210 215 220 Glu Ile Lys Lys Tyr Ala Ala Glu His Asn Ile Pro Leu Lys Ile Leu 225 230 235 240 Phe Leu Asn Asp Gly Pro Gly Leu Leu Leu Gly Ser Met Trp Asn Asp 245 250 255 Tyr Ser Ala Leu Glu Asp Tyr Gly Glu Gly Cys Val Met Val Cys Thr 260 265 270 Leu Lys Met Leu Glu Glu Arg Phe Thr Ile Asp Trp Leu Glu Asn Leu 275 280 285 631188DNABacillus subtilis 63atgaataaag acaatcaaat caaaaatgaa tctggtaaac aagccaaaat tcttgtatca 60gaaatcgtaa ataatcttaa aaatgaatta gggattaata tagaaattga agaagggtac 120tctataggtt acccaaatca agaaaagcaa tttaaaatgg attttcttgt tcaatttact 180gactttgata acgaacaatg gttaataaaa tcaactaact ctataaggga acgtatatac 240ggtacagaat tttttgcaca aaacatcagg cttatcgatg agaaagtaaa aaatatatat 300gttgttgttc cagattctat atcttcagct gaaatgaaaa agaaaagaaa ctactccgta 360aaaataaacg gaacaacata tacttccttt ttaactgatg ttttaaccgt taatgaattg 420cgacaaaaaa ttgtagaaaa ggcatctcaa aacatagcgc agggcttacg tgctaatgtg 480cttggtaatg atgctgaaac cagtattgtt aacctgctta atgatttgaa aaataaagca 540ttatggaatg attatcaaaa cgctcaacaa accatcaaat catcaacata caagatatac 600aaagagatcc ttgaaaaaat tgatctaaag gaaggctttg ataagatact tgaagttacc 660gctacaaatg atattcctct attatccaat aggggaaaac cgaaaacaga tgtatcagtt 720acaatcaaaa caaatacaaa agaattaatt aggaatatca gtataaaaaa cactcgtgaa 780aaaactgtca ctatacatga aggtagtgtt tcggatttga tttctcgatt aaaattatcg 840gaaacggacc cactatcgca agcacttata cattttgaaa aagtcggtag caaaaaaaaa 900ttaattgcag agcatcctaa ctcagataaa attttagagg aaaacttaaa attgtataat 960agagaactta ttgaattctt acatagccct ttactcaatg acaagataca aatggtagat 1020ttaattatat ttacaaataa atttgctgtt tggaatcgtg atgattatat taaacattac 1080atcgaagaat atagtggaaa aggacaattt ggaactcctt ttaaatggac ttatccaagc 1140aaaaagcgtg gtcaaaaaat acagattaaa ggtttttcaa acaattaa 118864395PRTBacillus subtilis 64Met Asn Lys Asp Asn Gln Ile Lys Asn Glu Ser Gly Lys Gln Ala Lys 1 5 10 15 Ile Leu Val Ser Glu Ile Val Asn Asn Leu Lys Asn Glu Leu Gly Ile 20 25 30 Asn Ile Glu Ile Glu Glu Gly Tyr Ser Ile Gly Tyr Pro Asn Gln Glu 35 40 45 Lys Gln Phe Lys Met Asp Phe Leu Val Gln Phe Thr Asp Phe Asp Asn 50 55 60 Glu Gln Trp Leu Ile Lys Ser Thr Asn Ser Ile Arg Glu Arg Ile Tyr 65 70 75 80 Gly Thr Glu Phe Phe Ala Gln Asn Ile Arg Leu Ile Asp Glu Lys Val 85 90 95 Lys Asn Ile Tyr Val Val Val Pro Asp Ser Ile Ser Ser Ala Glu Met 100 105 110 Lys Lys Lys Arg Asn Tyr Ser Val Lys Ile Asn Gly Thr Thr Tyr Thr 115 120 125 Ser Phe Leu Thr Asp Val Leu Thr Val Asn Glu Leu Arg Gln Lys Ile 130 135 140 Val Glu Lys Ala Ser Gln Asn Ile Ala Gln Gly Leu Arg Ala Asn Val 145 150 155 160 Leu Gly Asn Asp Ala Glu Thr Ser Ile Val Asn Leu Leu Asn Asp Leu 165 170 175 Lys Asn Lys Ala Leu Trp Asn Asp Tyr Gln Asn Ala Gln Gln Thr Ile 180 185 190 Lys Ser Ser Thr Tyr Lys Ile Tyr Lys Glu Ile Leu Glu Lys Ile Asp 195 200 205 Leu Lys Glu Gly Phe Asp Lys Ile Leu Glu Val Thr Ala Thr Asn Asp 210 215 220 Ile Pro Leu Leu Ser Asn Arg Gly Lys Pro Lys Thr Asp Val Ser Val 225 230 235 240 Thr Ile Lys Thr Asn Thr Lys Glu Leu Ile Arg Asn Ile Ser Ile Lys 245 250 255 Asn Thr Arg Glu Lys Thr Val Thr Ile His Glu Gly Ser Val Ser Asp 260 265 270 Leu Ile Ser Arg Leu Lys Leu Ser Glu Thr Asp Pro Leu Ser Gln Ala 275 280 285 Leu Ile His Phe Glu Lys Val Gly Ser Lys Lys Lys Leu Ile Ala Glu 290 295 300 His Pro Asn Ser Asp Lys Ile Leu Glu Glu Asn Leu Lys Leu Tyr Asn 305 310 315 320 Arg Glu Leu Ile Glu Phe Leu His Ser Pro Leu Leu Asn Asp Lys Ile 325 330 335 Gln Met Val Asp Leu Ile Ile Phe Thr Asn Lys Phe Ala Val Trp Asn 340 345 350 Arg Asp Asp Tyr Ile Lys His Tyr Ile Glu Glu Tyr Ser Gly Lys Gly 355 360 365 Gln Phe Gly Thr Pro Phe Lys Trp Thr Tyr Pro Ser Lys Lys Arg Gly 370 375 380 Gln Lys Ile Gln Ile Lys Gly Phe Ser Asn Asn 385 390 395 65933DNABacillus subtilis 36 65atgacaacct atatatatcc taccccacat aaagataaat tagttgccct attactaaac 60gataaattac cagtagaaga taaaccaaga gttgaagagg caattgtggt ttatacaaat 120tggataaaaa acttaaacat tattacaagt gccggtcttc ctccccaaca gactttaaat 180aaaatgattg agcttctaaa tgaatataaa ttctatatag atttaaattt ggtatttgat 240agcccaagag atttccttta tagacaaaaa gggcaattaa aaattgacaa tactattatt 300gaagaatttt taccccgttt agctcatccg tctgttattc ctgaaataat cgatatggat 360gtaacggttg gaccaaaaaa gtgtttttct tcagtttact ttgaatctag tcttgatgcg 420ccagcaattg gaggaggact aagagtaaga agcaaagacc aagactttgc aataagcaaa 480aaattattct taaaagcgtc acacacacaa gattataaag agagtttgga aacagaaaca 540ttcttatctt atgtgtctgc tgagtgtaaa acaaatcttg ataagacaat gtttcaagaa 600ggatgtgcta cagctcatga tacgaaggta gctgtaccag gttctaaata tttcttgcta 660tgtgaatggt tagatatgac accattaagt acagctccta cagatattga tgaaattcta 720cttctccgta aagccaaaag attaaattct aatataagaa aaaagttttc ttcttatagt 780gggagacaag aaaaacggga ttatttcatc aattatctca aatcacatcc atttagagta 840gaggtttttg aaagatttat tgaacacatt agaaaactta tccaaaatga agttccggtt 900gaacataatg ttatggaatt aggttatttt taa 93366310PRTBacillus subtilis 36 66Met Thr Thr Tyr Ile Tyr Pro Thr Pro His Lys Asp Lys Leu Val Ala 1 5 10 15 Leu Leu Leu Asn Asp Lys Leu Pro Val Glu Asp Lys Pro Arg Val Glu 20 25 30 Glu Ala Ile Val Val Tyr Thr Asn Trp Ile Lys Asn Leu Asn Ile Ile 35 40 45 Thr Ser Ala Gly Leu Pro Pro Gln Gln Thr Leu Asn Lys Met Ile Glu 50 55 60 Leu Leu Asn Glu Tyr Lys Phe Tyr Ile Asp Leu Asn Leu Val Phe Asp 65 70 75 80 Ser Pro Arg Asp Phe Leu Tyr Arg Gln Lys Gly Gln Leu Lys Ile Asp 85 90 95 Asn Thr Ile Ile Glu Glu Phe Leu Pro Arg Leu Ala His Pro Ser Val 100 105 110 Ile Pro Glu Ile Ile Asp Met Asp Val Thr Val Gly Pro Lys Lys Cys 115 120 125 Phe Ser Ser Val Tyr Phe Glu Ser Ser Leu Asp Ala Pro Ala Ile Gly 130 135 140 Gly Gly Leu Arg Val Arg Ser Lys Asp Gln Asp Phe Ala Ile Ser Lys 145 150 155 160 Lys Leu Phe Leu Lys Ala Ser His Thr Gln Asp Tyr Lys Glu Ser Leu 165 170 175 Glu Thr Glu Thr Phe Leu Ser Tyr Val Ser Ala Glu Cys Lys Thr Asn 180 185 190 Leu Asp Lys Thr Met Phe Gln Glu Gly Cys Ala Thr Ala His Asp Thr 195 200 205 Lys Val Ala Val Pro Gly Ser Lys Tyr Phe Leu Leu Cys Glu Trp Leu 210 215 220 Asp Met Thr Pro Leu Ser Thr Ala Pro Thr Asp Ile Asp Glu Ile Leu 225 230 235 240 Leu Leu Arg Lys Ala Lys Arg Leu Asn Ser Asn Ile Arg Lys Lys Phe 245 250 255 Ser Ser Tyr Ser Gly Arg Gln Glu Lys Arg Asp Tyr Phe Ile Asn Tyr 260 265 270 Leu Lys Ser His Pro Phe Arg Val Glu Val Phe Glu Arg Phe Ile Glu 275 280 285 His Ile Arg Lys Leu Ile Gln Asn Glu Val Pro Val Glu His Asn Val 290 295 300 Met Glu Leu Gly Tyr Phe 305 310 671161DNAClostridium acetobutylicum ABKn8 67atacatactt tatttgaaaa agaaattatt aactccaatc atgaatacta tataccacaa 60ttatctaatt caatagaaaa tttttattta ttgaatgcag atttaaatcg aataccaagc 120tcaacagcgg acatgctttt agttttccaa cgtttgtttg ataaagcact taaaaatgat 180tttacatcat tatcaataat taattacatg cataataatt taacagatga atctaaagct 240aaacgtaaag ttactgctag agatattgaa gattttattg ctgatctttt cgaaggaact 300gtaactgatg aagaaagtag acaaaatctc acttcaacta tagatattgt agactcttat 360atatcaagta actacaggga aaaatgtgat attcaattca ataattcata taaattatca 420ataaaaagct ttataagcga taacaaagaa atcaattgtg gttcttttgc tagagaagct 480ctatttaaag atatagttga aaattatggc ggtgaaagaa aaaatggatt agggtctaaa 540gggcaatttc tagatttatt tgaaaaaatc aaagataatg gaaaatggac agactttact 600aatcgcttta cttatatgac taataatata tttaaagatg acttattaat ttttattaaa 660ggtggtaata atgttgatat ctatttagtt gatagtgaaa aatttaggaa tacattaatt 720tctgctgttt catcagggcc taaatttgca gtttcagttt taaatagata tgaaggaaat 780tctatacgaa ttgaaagaga tattttcctt tcacctaaaa tcagtacaca tattggttta 840aattttaata aaactaatga aaatgccctt aataaaatag atgttgaact acaaaaactg 900aaagatgtga cattaaattt tatatcaaat gatactgctt ctttaaataa ttatagccaa 960ttaatatcta catttaactc ttcttatcag aatactattt ctgatttact ttcattgaaa 1020tcaatgactt tatcttcaga tgcattaatt acatctttcc atcaaaatgt tcttaatctt 1080tattcatcaa ataagttgtc aattattgat atgaaaaaga agaaaagagg aaattcatat 1140agtattgtaa gggaattata a 116168386PRTClostridium acetobutylicum ABKn8 68Met His Thr Leu Phe Glu Lys Glu Ile Ile Asn Ser Asn His Glu Tyr 1 5 10 15 Tyr Ile Pro Gln Leu Ser Asn Ser Ile Glu Asn Phe Tyr Leu Leu Asn 20 25 30 Ala Asp Leu Asn Arg Ile Pro Ser Ser Thr Ala Asp Met Leu Leu Val 35 40 45 Phe Gln Arg Leu Phe Asp Lys Ala Leu Lys Asn Asp Phe Thr Ser Leu 50 55 60 Ser Ile Ile Asn Tyr Met His Asn Asn Leu Thr Asp Glu

Ser Lys Ala 65 70 75 80 Lys Arg Lys Val Thr Ala Arg Asp Ile Glu Asp Phe Ile Ala Asp Leu 85 90 95 Phe Glu Gly Thr Val Thr Asp Glu Glu Ser Arg Gln Asn Leu Thr Ser 100 105 110 Thr Ile Asp Ile Val Asp Ser Tyr Ile Ser Ser Asn Tyr Arg Glu Lys 115 120 125 Cys Asp Ile Gln Phe Asn Asn Ser Tyr Lys Leu Ser Ile Lys Ser Phe 130 135 140 Ile Ser Asp Asn Lys Glu Ile Asn Cys Gly Ser Phe Ala Arg Glu Ala 145 150 155 160 Leu Phe Lys Asp Ile Val Glu Asn Tyr Gly Gly Glu Arg Lys Asn Gly 165 170 175 Leu Gly Ser Lys Gly Gln Phe Leu Asp Leu Phe Glu Lys Ile Lys Asp 180 185 190 Asn Gly Lys Trp Thr Asp Phe Thr Asn Arg Phe Thr Tyr Met Thr Asn 195 200 205 Asn Ile Phe Lys Asp Asp Leu Leu Ile Phe Ile Lys Gly Gly Asn Asn 210 215 220 Val Asp Ile Tyr Leu Val Asp Ser Glu Lys Phe Arg Asn Thr Leu Ile 225 230 235 240 Ser Ala Val Ser Ser Gly Pro Lys Phe Ala Val Ser Val Leu Asn Arg 245 250 255 Tyr Glu Gly Asn Ser Ile Arg Ile Glu Arg Asp Ile Phe Leu Ser Pro 260 265 270 Lys Ile Ser Thr His Ile Gly Leu Asn Phe Asn Lys Thr Asn Glu Asn 275 280 285 Ala Leu Asn Lys Ile Asp Val Glu Leu Gln Lys Leu Lys Asp Val Thr 290 295 300 Leu Asn Phe Ile Ser Asn Asp Thr Ala Ser Leu Asn Asn Tyr Ser Gln 305 310 315 320 Leu Ile Ser Thr Phe Asn Ser Ser Tyr Gln Asn Thr Ile Ser Asp Leu 325 330 335 Leu Ser Leu Lys Ser Met Thr Leu Ser Ser Asp Ala Leu Ile Thr Ser 340 345 350 Phe His Gln Asn Val Leu Asn Leu Tyr Ser Ser Asn Lys Leu Ser Ile 355 360 365 Ile Asp Met Lys Lys Lys Lys Arg Gly Asn Ser Tyr Ser Ile Val Arg 370 375 380 Glu Leu 385 691080DNACaryophanon latum L 69atgacttatt taattttaag gaggcaacaa cgtatgaaga actctgcaca aatgattaaa 60gataacatca tgaaagaaca gttaacaatt tatcatgaaa tcgaagtagg tgatcctgaa 120ttttggtact ctactgaaca aatggaagaa ctattaaatg aagctcttca aggcacagat 180ttgaacggga tggctttaag aactcgttca aagtttgtaa aagtcaaaat ttgtgaagct 240tttggatatc aggtgcccaa atcgtttaaa aaaacacaac cacgtttttt atctcaaaaa 300tttgatgtat ataatcaaaa atcaaataat ctccaaattt ggaatgaaga aatttctcct 360tcaagaagat atgttttaat aaaaatttct ttcgatgata ttattactca ggtgaaagta 420gttactggtg atgttttagc gacgttagat agtacaggaa cattaactca aaaatatcaa 480gcgaaatatg ctggtgtaca tgaaagaaag gctacacttc taagtgaatg cgatacagac 540tttattcaaa gcattactca atcatacaat agttttgacg aatttacagc tcctgataca 600aatccaaaag aagacgaatt aatgggaatt gacgaaattt ttgataagct aaaggattta 660atcggaacta agattccata tataggtgct actcaagaaa gaaatcgagg gggtcattta 720cacaagatga tttgtgatgc ccttggttat aataatttta aagagaacgg gcagtttcca 780gatataaaac atcaactatt agaagtgaag ctgcaaacgt cggaaactat agatttagga 840ttatttacgc ccaatagtta tgagctatta gacatccctc aattaaataa cgagtctatt 900tcaatgttag atgtgcgtta tgctatattt tatggtgatg ttatagaaga cactattact 960attacacatt tttatttagt tacaggtgaa gacttcttca cgtattttaa accctttggt 1020gggaaaggga ttaataagaa aattcaaatt cctttaaatg aagaattttg gaatctttaa 108070359PRTCaryophanon latum L 70Met Thr Tyr Leu Ile Leu Arg Arg Gln Gln Arg Met Lys Asn Ser Ala 1 5 10 15 Gln Met Ile Lys Asp Asn Ile Met Lys Glu Gln Leu Thr Ile Tyr His 20 25 30 Glu Ile Glu Val Gly Asp Pro Glu Phe Trp Tyr Ser Thr Glu Gln Met 35 40 45 Glu Glu Leu Leu Asn Glu Ala Leu Gln Gly Thr Asp Leu Asn Gly Met 50 55 60 Ala Leu Arg Thr Arg Ser Lys Phe Val Lys Val Lys Ile Cys Glu Ala 65 70 75 80 Phe Gly Tyr Gln Val Pro Lys Ser Phe Lys Lys Thr Gln Pro Arg Phe 85 90 95 Leu Ser Gln Lys Phe Asp Val Tyr Asn Gln Lys Ser Asn Asn Leu Gln 100 105 110 Ile Trp Asn Glu Glu Ile Ser Pro Ser Arg Arg Tyr Val Leu Ile Lys 115 120 125 Ile Ser Phe Asp Asp Ile Ile Thr Gln Val Lys Val Val Thr Gly Asp 130 135 140 Val Leu Ala Thr Leu Asp Ser Thr Gly Thr Leu Thr Gln Lys Tyr Gln 145 150 155 160 Ala Lys Tyr Ala Gly Val His Glu Arg Lys Ala Thr Leu Leu Ser Glu 165 170 175 Cys Asp Thr Asp Phe Ile Gln Ser Ile Thr Gln Ser Tyr Asn Ser Phe 180 185 190 Asp Glu Phe Thr Ala Pro Asp Thr Asn Pro Lys Glu Asp Glu Leu Met 195 200 205 Gly Ile Asp Glu Ile Phe Asp Lys Leu Lys Asp Leu Ile Gly Thr Lys 210 215 220 Ile Pro Tyr Ile Gly Ala Thr Gln Glu Arg Asn Arg Gly Gly His Leu 225 230 235 240 His Lys Met Ile Cys Asp Ala Leu Gly Tyr Asn Asn Phe Lys Glu Asn 245 250 255 Gly Gln Phe Pro Asp Ile Lys His Gln Leu Leu Glu Val Lys Leu Gln 260 265 270 Thr Ser Glu Thr Ile Asp Leu Gly Leu Phe Thr Pro Asn Ser Tyr Glu 275 280 285 Leu Leu Asp Ile Pro Gln Leu Asn Asn Glu Ser Ile Ser Met Leu Asp 290 295 300 Val Arg Tyr Ala Ile Phe Tyr Gly Asp Val Ile Glu Asp Thr Ile Thr 305 310 315 320 Ile Thr His Phe Tyr Leu Val Thr Gly Glu Asp Phe Phe Thr Tyr Phe 325 330 335 Lys Pro Phe Gly Gly Lys Gly Ile Asn Lys Lys Ile Gln Ile Pro Leu 340 345 350 Asn Glu Glu Phe Trp Asn Leu 355 712199DNAChlorella strain NC64A (CA-1A) 71atgtcttttc gcacgttaga actattcgcc ggtatagctg gtatttcaca tggcctcaga 60ggtatatcta caccagttgc attcgtagaa attaatgaag acgcacaaaa attcttgaaa 120acaaagtttt cagatgcatc tgtattcaat gacgttacga aatttaccaa atcggacttc 180ccagaagaca tagacatgat tactgcggga ttcccgtgca ctgggtttag tattgcaggt 240tctagaactg gattcgaaca caaggaatcc ggtctctttg ctgatgttgt gcgaatcacg 300gaagagtata aacctaaaat agtgtttttg gaaaactccc atatgttgtc ccacacttac 360aatctcgatg tcgtcgtaaa aaagatggat gaaattggtt atttctgcaa gtgggtaact 420tgtcgggcat caattatagg agcccatcat caacgccacc ggtggttttg tctcgcgatt 480cgaaaagatt atgaaccaga agaaataatt gtatctgtga atgctacaaa gttcgactgg 540gaaaataatg aaccaccgtg tcaagtagac aataagagtt acgagaattc aactcttgtt 600cgtctggcag gatattccgt ggtccccgac cagatcagat atgctttcac cggtctattt 660acaggtgatt ttgagtcatc gtggaaaact accttgacac ctgggacaat aattggcacg 720gaacacaaaa aaatgaaagg aacttacgat aaagtcataa acgggtatta tgagaacgat 780gtgtattatt ctttttcaag gaaagaagtt catcgcgctc ctctaaatat atccgtgaaa 840ccacgtgata ttccggagaa acataacgga aaaacactcg tagatcgcga aatgatcaag 900aaatattggt gcacaccatg tgctagttat ggcactgcta ctgctggatg caatgttctg 960acagaccgtc agtcacatgc acttcctaca caagtcaggt tttcatatag gggtgtatgt 1020ggacgacatt tgtctggtat atggtgtgca tggttgatgg ggtatgacca agaatatctt 1080ggttatttgg ttcaatatga ttaaaatatt ttgatacact aaatggatat aagaagaaaa 1140cgttttacaa tagaaggggc taaacgtata atactcgaaa aaaagagact tgaagagaaa 1200aaacgaattg cggaagagaa aaaaagaatt gcacttatag aaaaacaacg aattgcggaa 1260gagaaaaaaa gaattgcgga agagaaaaaa cgattcgcac ttgaagagaa aaaacgaatt 1320gcggaagaaa aaaaacgaat cgcggaagag aaaaaacgaa tcgtggaaga gaaaaaaaga 1380cttgcactta tagaaaaaca acgaattgcg gaagagaaaa ttgcgtcggg gagaaaaatt 1440agaaagagga tctctacaaa tgcaacaaaa catgaaagag aatttgtcaa agttataaat 1500tcaatgttcg tcggacccgc tacttttgta ttcgtagata taaaaggtaa taaatccaga 1560gaaatccaca acgttgtaag attcagacaa ttacaaggca gtaaagcgaa atccccgacc 1620gcgtatgttg atagagaata taacaaacct aaagcggata tagcagcggt agacataacc 1680ggtaaagatg tggcatggat atcccataaa gcatctgaag gatatcaaca atatctaaaa 1740atttctggaa agaacctcaa gttcacagga aaagaattag aagaagttct atcgttcaag 1800agaaaagtag ttagtatggc accggtatct aaaatatggc ctgctaataa gaccgtatgg 1860tctcctatca agtcaaattt gattaaaaat caagcaatat tcggatttga ttacggtaag 1920aaaccaggaa gggacaatgt agacatcata ggtcaaggac gaccaattat aacaaaaaga 1980agttccatat tatatcttac attcactggt tttagcgcat taaatgggca cttggagaat 2040tttactggga aacatgaacc cgttttctat gtaagaacag aacggagtag tagcgggaga 2100agtataacaa ctgtcgtcaa tggtgtcact tataaaaatt taagattctt tatacatcca 2160tacaactttg tttcttcaaa aacacaacgt attatgtag 219972278PRTChlorella strain NC64A (CA-1A) 72Met Glu Glu Lys Lys Arg Leu Ala Leu Ile Glu Lys Gln Arg Ile Ala 1 5 10 15 Glu Glu Lys Ile Ala Ser Gly Arg Lys Ile Arg Lys Arg Ile Ser Thr 20 25 30 Asn Ala Thr Lys His Glu Arg Glu Phe Val Lys Val Ile Asn Ser Met 35 40 45 Phe Val Gly Pro Ala Thr Phe Val Phe Val Asp Ile Lys Gly Asn Lys 50 55 60 Ser Arg Glu Ile His Asn Val Val Arg Phe Arg Gln Leu Gln Gly Ser 65 70 75 80 Lys Ala Lys Ser Pro Thr Ala Tyr Val Asp Arg Glu Tyr Asn Lys Pro 85 90 95 Lys Ala Asp Ile Ala Ala Val Asp Ile Thr Gly Lys Asp Val Ala Trp 100 105 110 Ile Ser His Lys Ala Ser Glu Gly Tyr Gln Gln Tyr Leu Lys Ile Ser 115 120 125 Gly Lys Asn Leu Lys Phe Thr Gly Lys Glu Leu Glu Glu Val Leu Ser 130 135 140 Phe Lys Arg Lys Val Val Ser Met Ala Pro Val Ser Lys Ile Trp Pro 145 150 155 160 Ala Asn Lys Thr Val Trp Ser Pro Ile Lys Ser Asn Leu Ile Lys Asn 165 170 175 Gln Ala Ile Phe Gly Phe Asp Tyr Gly Lys Lys Pro Gly Arg Asp Asn 180 185 190 Val Asp Ile Ile Gly Gln Gly Arg Pro Ile Ile Thr Lys Arg Ser Ser 195 200 205 Ile Leu Tyr Leu Thr Phe Thr Gly Phe Ser Ala Leu Asn Gly His Leu 210 215 220 Glu Asn Phe Thr Gly Lys His Glu Pro Val Phe Tyr Val Arg Thr Glu 225 230 235 240 Arg Ser Ser Ser Gly Arg Ser Ile Thr Thr Val Val Asn Gly Val Thr 245 250 255 Tyr Lys Asn Leu Arg Phe Phe Ile His Pro Tyr Asn Phe Val Ser Ser 260 265 270 Lys Thr Gln Arg Ile Met 275 731155DNADeinococcus radiophilus 73atgagtgaaa ttgacaactt ggtgaacttt atcctttcta aagatggaat tggtgacaaa 60tccattcttg agaaagaagt gattgaaaga ttttcattaa cgagggatag gtctgtttac 120tactgcacag attttgctat acgatttagt tcctcgaaat cagcagcatt tagcaacaca 180gtcctatcgc tatccaatct cagaaaattt gatagcaagc cctttattgt ttgcctcata 240actcctgcaa aaaattacct ttttttggca aacactagct ttctgaaaaa aatcagccat 300agttcacaga ccttaacgag caacaatatt agaggcagtt tcaatggaag cgacatatat 360aaggatttcg atggtatacc caattcccct gagaacttcg aatatctgtt tagaatacac 420gcagaaacta catttgaaga gaatctaatt cgtttagcag aagcaaccaa cgatattgca 480cctagcggta agaagttcgt tccctcaccc caaggtgaag aaaatatata tctagccccc 540aagagagcaa gtgagtttat cgcctccgat aattacaggc agctgctgca agaattggat 600gatatagtaa ggcattatac caatgaaatc attattgcat ccatgataaa caacgtaaat 660atcaggggca gagtaatcga atatctagtg gccggagaag atgatcttct gagacaaaac 720ataatttata agctcagaaa tggcggtaca aatctacccc aattcaaaac agataattcg 780ttaggagatt actcaaaagc ttttgaaggc tttgatacag aaacagatgt gaaaacaaaa 840attatgctcc ttaattccaa tccaaaagca tacaacttag ataagattct taatttctta 900tcgagcgata agagcgtatt tcttttctat ttcattggaa tagattctga taactctctt 960aagacatgtc ttgtgactat gtttaatgag gagttgttac gaggtacaat tactctcagg 1020cattgggcag gcaggaattc tagaggcgtt tcccagttcg atggaaaaat catcaacaat 1080ataattctta atccgtcaaa taaaattgat aaggctcaag ctcgggaatt ccttaccaga 1140attttatctt tataa 115574384PRTDeinococcus radiophilus 74Met Ser Glu Ile Asp Asn Leu Val Asn Phe Ile Leu Ser Lys Asp Gly 1 5 10 15 Ile Gly Asp Lys Ser Ile Leu Glu Lys Glu Val Ile Glu Arg Phe Ser 20 25 30 Leu Thr Arg Asp Arg Ser Val Tyr Tyr Cys Thr Asp Phe Ala Ile Arg 35 40 45 Phe Ser Ser Ser Lys Ser Ala Ala Phe Ser Asn Thr Val Leu Ser Leu 50 55 60 Ser Asn Leu Arg Lys Phe Asp Ser Lys Pro Phe Ile Val Cys Leu Ile 65 70 75 80 Thr Pro Ala Lys Asn Tyr Leu Phe Leu Ala Asn Thr Ser Phe Leu Lys 85 90 95 Lys Ile Ser His Ser Ser Gln Thr Leu Thr Ser Asn Asn Ile Arg Gly 100 105 110 Ser Phe Asn Gly Ser Asp Ile Tyr Lys Asp Phe Asp Gly Ile Pro Asn 115 120 125 Ser Pro Glu Asn Phe Glu Tyr Leu Phe Arg Ile His Ala Glu Thr Thr 130 135 140 Phe Glu Glu Asn Leu Ile Arg Leu Ala Glu Ala Thr Asn Asp Ile Ala 145 150 155 160 Pro Ser Gly Lys Lys Phe Val Pro Ser Pro Gln Gly Glu Glu Asn Ile 165 170 175 Tyr Leu Ala Pro Lys Arg Ala Ser Glu Phe Ile Ala Ser Asp Asn Tyr 180 185 190 Arg Gln Leu Leu Gln Glu Leu Asp Asp Ile Val Arg His Tyr Thr Asn 195 200 205 Glu Ile Ile Ile Ala Ser Met Ile Asn Asn Val Asn Ile Arg Gly Arg 210 215 220 Val Ile Glu Tyr Leu Val Ala Gly Glu Asp Asp Leu Leu Arg Gln Asn 225 230 235 240 Ile Ile Tyr Lys Leu Arg Asn Gly Gly Thr Asn Leu Pro Gln Phe Lys 245 250 255 Thr Asp Asn Ser Leu Gly Asp Tyr Ser Lys Ala Phe Glu Gly Phe Asp 260 265 270 Thr Glu Thr Asp Val Lys Thr Lys Ile Met Leu Leu Asn Ser Asn Pro 275 280 285 Lys Ala Tyr Asn Leu Asp Lys Ile Leu Asn Phe Leu Ser Ser Asp Lys 290 295 300 Ser Val Phe Leu Phe Tyr Phe Ile Gly Ile Asp Ser Asp Asn Ser Leu 305 310 315 320 Lys Thr Cys Leu Val Thr Met Phe Asn Glu Glu Leu Leu Arg Gly Thr 325 330 335 Ile Thr Leu Arg His Trp Ala Gly Arg Asn Ser Arg Gly Val Ser Gln 340 345 350 Phe Asp Gly Lys Ile Ile Asn Asn Ile Ile Leu Asn Pro Ser Asn Lys 355 360 365 Ile Asp Lys Ala Gln Ala Arg Glu Phe Leu Thr Arg Ile Leu Ser Leu 370 375 380 75906DNAEnterobacter agglomerans 75atgaaaaaaa gaagagattt ggttgaagta tttggctata accctatgga ccttagccct 60gaagtcaggg ctctttggaa cttgggagca tgcccatttc ttaataaaga atgcataaaa 120ataaatcatg atcaaacaat aatttatggc acatgcagtg taacgtctcc ttatggagac 180gttattattt gtccaaatag gctttatgct aatgactatg aaaccttgca taaagtcagt 240cgcgatgcat ttggcgatga tgtccctttt ttgacttata gtaatttcat aaaatatagg 300gcgacttaca aagactgtat cgtagccctc ggtaaaaact cagggaaaga agttcaagtt 360ggcagggctc tatcgatgga ctgggttttg gtcagaatca ctgacgggga acttaaagaa 420tacgtaggcg tagaaataca aagcattgat ataactggaa attacagaga tgcttggcat 480gcttacaaaa acctcaaacc tatagatatc attgataact taccaacttc acaacatgga 540ctgaattggg ctaatgtaca caaaagactc ataccacaaa taataagaaa aggagttgtt 600tactctcgat caaattatgt aaaaaaaggt ctttatttta tattacctga gattgtctat 660aataaatttg aagatgttat tggtgcagac atacctcttt tgaaaacaca aacgaataaa 720agcataacag ttcatacata ctccttaggt gagccagctg caaatggtga acaacgaaaa 780ctaatcagtg aaagagaaat cattttcgat ttagacgaat tttcaaaaag attcacgact 840ggccccaact tgccaaaagg agatgatttg gacgcagtaa ttaaaaaagc gttaggaatg 900atgtaa 90676301PRTEnterobacter agglomerans 76Met Lys Lys Arg Arg Asp Leu Val Glu Val Phe Gly Tyr Asn Pro Met 1 5 10 15 Asp Leu Ser Pro Glu Val Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro 20 25 30 Phe Leu Asn Lys Glu Cys Ile Lys Ile Asn His Asp Gln Thr Ile Ile 35 40 45 Tyr Gly Thr Cys Ser Val Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys 50 55 60 Pro Asn Arg Leu Tyr Ala Asn Asp Tyr Glu Thr Leu His Lys Val Ser 65 70 75 80 Arg Asp Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe 85

90 95 Ile Lys Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys 100 105 110 Asn Ser Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp 115 120 125 Val Leu Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val 130 135 140 Glu Ile Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His 145 150 155 160 Ala Tyr Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr 165 170 175 Ser Gln His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro 180 185 190 Gln Ile Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys 195 200 205 Lys Gly Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu 210 215 220 Asp Val Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys 225 230 235 240 Ser Ile Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly 245 250 255 Glu Gln Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp 260 265 270 Glu Phe Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp 275 280 285 Asp Leu Asp Ala Val Ile Lys Lys Ala Leu Gly Met Met 290 295 300 77378DNAEnterobacter aerogenes 77gatcatacat tgcctgtata ttacttatgg cctttgacta ctaataatgc cacattgctc 60tgtaaagtac ataatggaga aaaagcagag aaatggcctg gcgagtttta ttcaaggcaa 120gaattggcat cactctcaag attgaccggg gttgaagctc gtgttttggc tggggcacca 180atatttaatc cagaagcaat tgatatttta aaaaatccta aattcgttga aggtttagtc 240gataagtttt ccagatatcc gaatgaggta tataatttac gcaatcgaat taagagagtt 300acagggttcg atttcttcga taaccctaat ttgaaaattt ctgccaattg ggttatcgaa 360gccgataaac ttatctaa 37878125PRTEnterobacter aerogenes 78Asp His Thr Leu Pro Val Tyr Tyr Leu Trp Pro Leu Thr Thr Asn Asn 1 5 10 15 Ala Thr Leu Leu Cys Lys Val His Asn Gly Glu Lys Ala Glu Lys Trp 20 25 30 Pro Gly Glu Phe Tyr Ser Arg Gln Glu Leu Ala Ser Leu Ser Arg Leu 35 40 45 Thr Gly Val Glu Ala Arg Val Leu Ala Gly Ala Pro Ile Phe Asn Pro 50 55 60 Glu Ala Ile Asp Ile Leu Lys Asn Pro Lys Phe Val Glu Gly Leu Val 65 70 75 80 Asp Lys Phe Ser Arg Tyr Pro Asn Glu Val Tyr Asn Leu Arg Asn Arg 85 90 95 Ile Lys Arg Val Thr Gly Phe Asp Phe Phe Asp Asn Pro Asn Leu Lys 100 105 110 Ile Ser Ala Asn Trp Val Ile Glu Ala Asp Lys Leu Ile 115 120 125 79696DNAUnknownEnvironmental sample BC3 79atgctcaaga ccacctcaac cttttccccc tcaaccgtca aattccccaa aaaacccgcg 60ctcacaatat ccttcccatc caccattata ccctttaaga tctccaaagc cttctttgat 120gtcatacata gaattctaaa ggcggagagg caatgctttc ccaactttat aataacgaga 180aggaaaatgc taagcttaaa ccttagggga ttatcaaaac cttctttgat agccctttac 240atagaccttc tgacgctcta ctttaaaacc accttgtggg tgtgcggttt tcaacccaac 300acggaaaaac ttggatacaa cggttatagg atggatgcag atacaggcaa gagaattgat 360tgcgaggtaa aaccacaaaa taccgataac cgtagaaaaa aattgactgg aggtggaagt 420tttaacgatt atacggtaga aaggtttaaa aaggatttag aaaacaatcc tgcaattttg 480gttagtggtt ttgtaggagg gaaactcata tacatctttg agtttaggtt tgaatgctta 540agggaaaaac ttaaaggttt gcttgaacgt agatttccaa ggggccacag aagggaaggt 600gaatacttac gttccgcgaa cttttctttc gatactttga gggtcttgaa agatgaaggg 660tttggaaagg ttcataaatc aagttataca cggtga 69680231PRTEnvironmental sample BC3 80Met Leu Lys Thr Thr Ser Thr Phe Ser Pro Ser Thr Val Lys Phe Pro 1 5 10 15 Lys Lys Pro Ala Leu Thr Ile Ser Phe Pro Ser Thr Ile Ile Pro Phe 20 25 30 Lys Ile Ser Lys Ala Phe Phe Asp Val Ile His Arg Ile Leu Lys Ala 35 40 45 Glu Arg Gln Cys Phe Pro Asn Phe Ile Ile Thr Arg Arg Lys Met Leu 50 55 60 Ser Leu Asn Leu Arg Gly Leu Ser Lys Pro Ser Leu Ile Ala Leu Tyr 65 70 75 80 Ile Asp Leu Leu Thr Leu Tyr Phe Lys Thr Thr Leu Trp Val Cys Gly 85 90 95 Phe Gln Pro Asn Thr Glu Lys Leu Gly Tyr Asn Gly Tyr Arg Met Asp 100 105 110 Ala Asp Thr Gly Lys Arg Ile Asp Cys Glu Val Lys Pro Gln Asn Thr 115 120 125 Asp Asn Arg Arg Lys Lys Leu Thr Gly Gly Gly Ser Phe Asn Asp Tyr 130 135 140 Thr Val Glu Arg Phe Lys Lys Asp Leu Glu Asn Asn Pro Ala Ile Leu 145 150 155 160 Val Ser Gly Phe Val Gly Gly Lys Leu Ile Tyr Ile Phe Glu Phe Arg 165 170 175 Phe Glu Cys Leu Arg Glu Lys Leu Lys Gly Leu Leu Glu Arg Arg Phe 180 185 190 Pro Arg Gly His Arg Arg Glu Gly Glu Tyr Leu Arg Ser Ala Asn Phe 195 200 205 Ser Phe Asp Thr Leu Arg Val Leu Lys Asp Glu Gly Phe Gly Lys Val 210 215 220 His Lys Ser Ser Tyr Thr Arg 225 230 811047DNAEnvironmental sample BC4 81atggacctgt tggctgaaat aagaggtata aaatataagc cgtttctttg tagagacctg 60gtaccttttg agtttagcag gttagaagat gctattgcct cctctccatc ttttatttta 120gaagttgatg aaaaaaatag ggttgctgta agttggtggg tttcacctaa acgcactcgc 180tcctatccat acgcaagagt ttacgatact ttaggttttt ccggaaagaa aataacaatc 240attccaatca tgaaagacga aggtgaagga ggggatagag attttttaca atgggatact 300gtttcactta tgagtttgtt aggagtttat gtgattattg cttattataa caaggccgag 360ccgagtaaaa ggtataaaaa caagatcaca aatcaacgat ttgatattga ttacattaaa 420gaaaaaatta aaagcattat ttcttaccaa tcggatgctt tacactggaa tctttatgaa 480gttgaaaatg tgggagaaat tggagaaaga gctctgaagg cttatgactt aatttcaatg 540gagttaaata taagaatgca ttcacgaaaa actgcagaaa aaagaattaa agaattatta 600aaagggaaag aaaagtttat gagtctttca cgaactttag cggagaaagc acaaagaaga 660gagaaattga ctattcagcc aaaagaaaat ctttctggat caaaagcttc tattacaata 720aagaattatc taggtggttt ttattatctc actgttgatg aagtaaaagt tataggaaat 780aaagttttgc taattgaggc gaagcatagc aagacaaatt ccttaccgtc gctggaagat 840ataaaagatg gtctgttaaa gatgatttta tttactaatc tcgaaaatgt agaaatagaa 900agtaaaaatt ataagccaga agctgtgtta aaactaactg ttgaaggtgg ttttaacgag 960agtagacttt caccttcaca aaaaaagact ttaaaactct tacaagaaga agccgaaagc 1020aataattttc aaattcaact aatatga 104782348PRTEnvironmental sample BC4 82Met Asp Leu Leu Ala Glu Ile Arg Gly Ile Lys Tyr Lys Pro Phe Leu 1 5 10 15 Cys Arg Asp Leu Val Pro Phe Glu Phe Ser Arg Leu Glu Asp Ala Ile 20 25 30 Ala Ser Ser Pro Ser Phe Ile Leu Glu Val Asp Glu Lys Asn Arg Val 35 40 45 Ala Val Ser Trp Trp Val Ser Pro Lys Arg Thr Arg Ser Tyr Pro Tyr 50 55 60 Ala Arg Val Tyr Asp Thr Leu Gly Phe Ser Gly Lys Lys Ile Thr Ile 65 70 75 80 Ile Pro Ile Met Lys Asp Glu Gly Glu Gly Gly Asp Arg Asp Phe Leu 85 90 95 Gln Trp Asp Thr Val Ser Leu Met Ser Leu Leu Gly Val Tyr Val Ile 100 105 110 Ile Ala Tyr Tyr Asn Lys Ala Glu Pro Ser Lys Arg Tyr Lys Asn Lys 115 120 125 Ile Thr Asn Gln Arg Phe Asp Ile Asp Tyr Ile Lys Glu Lys Ile Lys 130 135 140 Ser Ile Ile Ser Tyr Gln Ser Asp Ala Leu His Trp Asn Leu Tyr Glu 145 150 155 160 Val Glu Asn Val Gly Glu Ile Gly Glu Arg Ala Leu Lys Ala Tyr Asp 165 170 175 Leu Ile Ser Met Glu Leu Asn Ile Arg Met His Ser Arg Lys Thr Ala 180 185 190 Glu Lys Arg Ile Lys Glu Leu Leu Lys Gly Lys Glu Lys Phe Met Ser 195 200 205 Leu Ser Arg Thr Leu Ala Glu Lys Ala Gln Arg Arg Glu Lys Leu Thr 210 215 220 Ile Gln Pro Lys Glu Asn Leu Ser Gly Ser Lys Ala Ser Ile Thr Ile 225 230 235 240 Lys Asn Tyr Leu Gly Gly Phe Tyr Tyr Leu Thr Val Asp Glu Val Lys 245 250 255 Val Ile Gly Asn Lys Val Leu Leu Ile Glu Ala Lys His Ser Lys Thr 260 265 270 Asn Ser Leu Pro Ser Leu Glu Asp Ile Lys Asp Gly Leu Leu Lys Met 275 280 285 Ile Leu Phe Thr Asn Leu Glu Asn Val Glu Ile Glu Ser Lys Asn Tyr 290 295 300 Lys Pro Glu Ala Val Leu Lys Leu Thr Val Glu Gly Gly Phe Asn Glu 305 310 315 320 Ser Arg Leu Ser Pro Ser Gln Lys Lys Thr Leu Lys Leu Leu Gln Glu 325 330 335 Glu Ala Glu Ser Asn Asn Phe Gln Ile Gln Leu Ile 340 345 83711DNAEnvironmental sample BS #9 83atggcagaga gaggtttgag ttggcggttg gcaactatca acgaactcgg ttggcggggc 60aaaaaccgaa agcagcgact ctggtgcaac gccgcgacgg gtcgttctat ctgcaaatta 120gttgaaaaaa tccaacacaa acttccaaag ctatttcact tagcagaatt agagagttca 180agagctggta agatagggat ggaagtaggt tctattaggg aaaagattat cgtcgctttg 240ttgatacaca agtttggtga agaaaatgtt aagaccgata ttccgattac tgaagcagaa 300gtagatgttg aagtatttgg aaatccgctt tccataaaga ctattacagg aaaaaatcta 360tcgggtgtaa agctaatatg gactgttgat gccgctaaat caaaagaatt tcgtgagact 420tatttacctt tctgcgatat gattttaata caggtcaact ggggtagtaa cggcggattt 480taccttattc caaaagaaac gcaacttgac gtattgaaca acttaggcag aacaacatac 540ataaaactcc ccaaaactgg gacaaatcca agaggcgttg agttgagcag gcaggcttta 600caggagttag tgcggcacaa agacacaatg ataataccta ttgattggaa aaaagaagaa 660atagacttta aaccacttaa aagatggatt gaattatggg agaaagagta a 71184236PRTEnvironmental sample BS #9 84Met Ala Glu Arg Gly Leu Ser Trp Arg Leu Ala Thr Ile Asn Glu Leu 1 5 10 15 Gly Trp Arg Gly Lys Asn Arg Lys Gln Arg Leu Trp Cys Asn Ala Ala 20 25 30 Thr Gly Arg Ser Ile Cys Lys Leu Val Glu Lys Ile Gln His Lys Leu 35 40 45 Pro Lys Leu Phe His Leu Ala Glu Leu Glu Ser Ser Arg Ala Gly Lys 50 55 60 Ile Gly Met Glu Val Gly Ser Ile Arg Glu Lys Ile Ile Val Ala Leu 65 70 75 80 Leu Ile His Lys Phe Gly Glu Glu Asn Val Lys Thr Asp Ile Pro Ile 85 90 95 Thr Glu Ala Glu Val Asp Val Glu Val Phe Gly Asn Pro Leu Ser Ile 100 105 110 Lys Thr Ile Thr Gly Lys Asn Leu Ser Gly Val Lys Leu Ile Trp Thr 115 120 125 Val Asp Ala Ala Lys Ser Lys Glu Phe Arg Glu Thr Tyr Leu Pro Phe 130 135 140 Cys Asp Met Ile Leu Ile Gln Val Asn Trp Gly Ser Asn Gly Gly Phe 145 150 155 160 Tyr Leu Ile Pro Lys Glu Thr Gln Leu Asp Val Leu Asn Asn Leu Gly 165 170 175 Arg Thr Thr Tyr Ile Lys Leu Pro Lys Thr Gly Thr Asn Pro Arg Gly 180 185 190 Val Glu Leu Ser Arg Gln Ala Leu Gln Glu Leu Val Arg His Lys Asp 195 200 205 Thr Met Ile Ile Pro Ile Asp Trp Lys Lys Glu Glu Ile Asp Phe Lys 210 215 220 Pro Leu Lys Arg Trp Ile Glu Leu Trp Glu Lys Glu 225 230 235 85780DNAEnvironmental sample Dixie, Nevada 85atgaaagatc caattgaaga cctaaaaagg tatcgtgatt ttcttgaaag cataccttta 60gacgagtata gaaaacaatt aaaaggtatt aaatgggtag agcaagactt acccaaagaa 120attttacccc ttgcctcaat attcaaatat tactgggaga ttagaaaatt tttaaatttt 180gatgaatggt tcgataaatt ttggagagag ataaatacca atctagaaag taagaagaca 240cttgaagaat tcaaaagata tttcttcaat aagtcacttg aagaaaatga ttggtttagg 300aaaggattta aagcaagaat gtatagaact tgggtatctg ttcttactca attagacttt 360tgttatatgt ttgaatatgt ctgtgctaaa aagggaataa atttaaaatt agagtgcaat 420gcagagttag atgcaagagg aattgatgct aaggttaatg atatttgttt tcaggtagcg 480aaaataagtc aaagaaaaga agcaaggact gtaggtagaa agaaaacaat tattactata 540ccttatgctg tatttaacat agaagagttt aaaagaagga ttgcaagtcc gcgggttaaa 600gacaaaagta gctatcaaaa agccttaaag gcgtttcata agtactttga tcttcttaaa 660aatggctttg ttgtttttaa gaaagattat ataaagaaga taataaataa catagacgat 720gttgaaaaac tgagacaagc ggttaatgaa atctcacgag aattatgtgg agaaatttaa 78086259PRTEnvironmental sample Dixie, Nevada 86Met Lys Asp Pro Ile Glu Asp Leu Lys Arg Tyr Arg Asp Phe Leu Glu 1 5 10 15 Ser Ile Pro Leu Asp Glu Tyr Arg Lys Gln Leu Lys Gly Ile Lys Trp 20 25 30 Val Glu Gln Asp Leu Pro Lys Glu Ile Leu Pro Leu Ala Ser Ile Phe 35 40 45 Lys Tyr Tyr Trp Glu Ile Arg Lys Phe Leu Asn Phe Asp Glu Trp Phe 50 55 60 Asp Lys Phe Trp Arg Glu Ile Asn Thr Asn Leu Glu Ser Lys Lys Thr 65 70 75 80 Leu Glu Glu Phe Lys Arg Tyr Phe Phe Asn Lys Ser Leu Glu Glu Asn 85 90 95 Asp Trp Phe Arg Lys Gly Phe Lys Ala Arg Met Tyr Arg Thr Trp Val 100 105 110 Ser Val Leu Thr Gln Leu Asp Phe Cys Tyr Met Phe Glu Tyr Val Cys 115 120 125 Ala Lys Lys Gly Ile Asn Leu Lys Leu Glu Cys Asn Ala Glu Leu Asp 130 135 140 Ala Arg Gly Ile Asp Ala Lys Val Asn Asp Ile Cys Phe Gln Val Ala 145 150 155 160 Lys Ile Ser Gln Arg Lys Glu Ala Arg Thr Val Gly Arg Lys Lys Thr 165 170 175 Ile Ile Thr Ile Pro Tyr Ala Val Phe Asn Ile Glu Glu Phe Lys Arg 180 185 190 Arg Ile Ala Ser Pro Arg Val Lys Asp Lys Ser Ser Tyr Gln Lys Ala 195 200 205 Leu Lys Ala Phe His Lys Tyr Phe Asp Leu Leu Lys Asn Gly Phe Val 210 215 220 Val Phe Lys Lys Asp Tyr Ile Lys Lys Ile Ile Asn Asn Ile Asp Asp 225 230 235 240 Val Glu Lys Leu Arg Gln Ala Val Asn Glu Ile Ser Arg Glu Leu Cys 245 250 255 Gly Glu Ile 87969DNABacillus species 2521 87atggttatta atcatttatt attgcctaat ttaaatatta ataatgaaaa agcagtcccg 60gaagtatata aacgtatttt ggaagggtat ttagattatt taaatacagc cctcgaatac 120gaatcaattg ctatgtctga ggtagttgct ggagttataa gtgaattaat tttatataat 180gaaattaagc atgactggtt tttaattata aaagacttac tagaatatga tgaattaccg 240atatcttatt ctaagaatta tggtgaaaaa ttatatggat ttaattcaca gtggttacaa 300catactgttc atgccactta taatcatagt tttattatga atttgttaaa taagagccaa 360tttgattact caagtattat attagattta gttcaacctg atggatatat ttataacaag 420aaggttagtg caaccaatcc ccgaacccgc atgaaaagtg agctattaat gtctttaact 480atggggttat cgttaattga ttctagtcga attcctgaac agtgtatcgt taagataaag 540acatttgata aaacagaatt tgtaacagca gagtatttta agttgttctg tttaaagctt 600ttgaaaatag ataacttaga aacgtattgc aactacaatg atatattatt agaaagatgt 660tttaccggta ctggatatgc tgattttaat gttcaagata aagtcgatga ttacatggga 720acattaaaac gaactgctag agataaatct gttgcatcac ccttaataac ggtttacgca 780ggagaaattg ctgaagtatt aggttcttct acgttagatt tgtataattc taacaaggaa 840aagtatattc aacatttatc tttgaacccg ctagacatta ccgcttataa aatgagagat 900cttaatgcag attttgggga aagtattacc ccttttgaaa ttttttccac tataattctg 960aataattaa 96988322PRTBacillus species 2521 88Met Val Ile Asn His Leu Leu Leu Pro Asn Leu Asn Ile Asn Asn Glu 1 5 10 15 Lys Ala Val Pro Glu Val Tyr Lys Arg Ile Leu Glu Gly Tyr Leu Asp 20 25 30 Tyr Leu Asn Thr Ala Leu Glu Tyr Glu Ser Ile Ala Met Ser Glu Val 35 40 45 Val Ala Gly Val Ile Ser Glu Leu Ile Leu Tyr Asn Glu Ile Lys His 50 55 60 Asp Trp Phe Leu Ile Ile Lys Asp Leu Leu Glu Tyr Asp Glu Leu Pro 65 70 75 80 Ile Ser Tyr Ser Lys Asn Tyr Gly Glu Lys Leu Tyr Gly Phe Asn Ser 85 90 95 Gln Trp Leu Gln His Thr Val His Ala Thr Tyr Asn His Ser Phe Ile 100 105

110 Met Asn Leu Leu Asn Lys Ser Gln Phe Asp Tyr Ser Ser Ile Ile Leu 115 120 125 Asp Leu Val Gln Pro Asp Gly Tyr Ile Tyr Asn Lys Lys Val Ser Ala 130 135 140 Thr Asn Pro Arg Thr Arg Met Lys Ser Glu Leu Leu Met Ser Leu Thr 145 150 155 160 Met Gly Leu Ser Leu Ile Asp Ser Ser Arg Ile Pro Glu Gln Cys Ile 165 170 175 Val Lys Ile Lys Thr Phe Asp Lys Thr Glu Phe Val Thr Ala Glu Tyr 180 185 190 Phe Lys Leu Phe Cys Leu Lys Leu Leu Lys Ile Asp Asn Leu Glu Thr 195 200 205 Tyr Cys Asn Tyr Asn Asp Ile Leu Leu Glu Arg Cys Phe Thr Gly Thr 210 215 220 Gly Tyr Ala Asp Phe Asn Val Gln Asp Lys Val Asp Asp Tyr Met Gly 225 230 235 240 Thr Leu Lys Arg Thr Ala Arg Asp Lys Ser Val Ala Ser Pro Leu Ile 245 250 255 Thr Val Tyr Ala Gly Glu Ile Ala Glu Val Leu Gly Ser Ser Thr Leu 260 265 270 Asp Leu Tyr Asn Ser Asn Lys Glu Lys Tyr Ile Gln His Leu Ser Leu 275 280 285 Asn Pro Leu Asp Ile Thr Ala Tyr Lys Met Arg Asp Leu Asn Ala Asp 290 295 300 Phe Gly Glu Ser Ile Thr Pro Phe Glu Ile Phe Ser Thr Ile Ile Leu 305 310 315 320 Asn Asn 89603DNAEnvironmental sample LHC 89gcaaagggaa acatcaattc attcaaaggc attcacaaag ttttccatga attcgagcca 60attcagacca ctgtggtatg gccttacaaa aagagcttac ttaacgagta caatacaagc 120gaaagcaaca ttttatctgt agcaaataat caaagaattt tgcatcactt cttgtttgga 180aaagatacag aatttgatag cttagacatt acaaaacgac ctaaaaccta ttttccacat 240agaacaaaaa tgagtttttt ctatagcttt ggaaaagatt tacagattga gttgaagaac 300atacaaatag aaattgattt aactattgag tttcaaggca taatcggaat ttttgaagca 360aagaatggca gtcctagtaa ttttgcaatt tatcagcttt atcatccttt tttatactat 420tacaacgcca atcaaatctc cgagataaaa ggcgaaatca aaaacattta tggtgtttat 480gttgttagaa acatagaacg taggattaca aacctaaaaa tgtgggcata tacttttgag 540aatccattgg atattactag tataaagttt gtaaaatctg cttgctacca actaaaagtc 600taa 60390200PRTEnvironmental sample LHC 90Ala Lys Gly Asn Ile Asn Ser Phe Lys Gly Ile His Lys Val Phe His 1 5 10 15 Glu Phe Glu Pro Ile Gln Thr Thr Val Val Trp Pro Tyr Lys Lys Ser 20 25 30 Leu Leu Asn Glu Tyr Asn Thr Ser Glu Ser Asn Ile Leu Ser Val Ala 35 40 45 Asn Asn Gln Arg Ile Leu His His Phe Leu Phe Gly Lys Asp Thr Glu 50 55 60 Phe Asp Ser Leu Asp Ile Thr Lys Arg Pro Lys Thr Tyr Phe Pro His 65 70 75 80 Arg Thr Lys Met Ser Phe Phe Tyr Ser Phe Gly Lys Asp Leu Gln Ile 85 90 95 Glu Leu Lys Asn Ile Gln Ile Glu Ile Asp Leu Thr Ile Glu Phe Gln 100 105 110 Gly Ile Ile Gly Ile Phe Glu Ala Lys Asn Gly Ser Pro Ser Asn Phe 115 120 125 Ala Ile Tyr Gln Leu Tyr His Pro Phe Leu Tyr Tyr Tyr Asn Ala Asn 130 135 140 Gln Ile Ser Glu Ile Lys Gly Glu Ile Lys Asn Ile Tyr Gly Val Tyr 145 150 155 160 Val Val Arg Asn Ile Glu Arg Arg Ile Thr Asn Leu Lys Met Trp Ala 165 170 175 Tyr Thr Phe Glu Asn Pro Leu Asp Ile Thr Ser Ile Lys Phe Val Lys 180 185 190 Ser Ala Cys Tyr Gln Leu Lys Val 195 200 91753DNAEnvironmental sample S1 91ttaaacttag aaccccgtat tgttgaaagt acaactgaca ttttagagtt gcttatacaa 60accgactcca aagggagaga aggtgatgta cgagatatat taataattcg gcgagatatt 120cagtgggaaa tagggctaag ccttaagcat aatcattttg ctgtaaagca tagccgtcta 180agtcgaaaat tggattttgg aaatgaatgg tatggcattt catgctcgga ggcttattgg 240aaaggagtta atcctgtatt tgattatcta gttgtcgaaa aaagtaaaca taaaaaattc 300aatgaactca aaaataaaga agaagtcgtt tacgttcctt tgctaaaagc ttttatagat 360gaaatcaagc aacaatgcca agtccataaa gatattccta gtaaattggt acaatacctt 420ttaggaaagt atgactttta taaaataatt agcatagata aagagcggat gactcaaatt 480caatcctata atttacacgg tacactcaac aaaaacagcg aatcgaaaaa agcatccatt 540cgaattccac tagcatcctt gccaacgcgt atagtgagtt tggactttgt tccggggaag 600acaaacactg ttgaacttta tatggatggt ggttggcaat tttcttttcg catacataac 660gcagaaactt atgttgcgcc gaccttgaag tttgatattc aaatagtagg tatgcctact 720gctataatca caataaattg tctttggaaa taa 75392250PRTEnvironmental sample S1 92Met Asn Leu Glu Pro Arg Ile Val Glu Ser Thr Thr Asp Ile Leu Glu 1 5 10 15 Leu Leu Ile Gln Thr Asp Ser Lys Gly Arg Glu Gly Asp Val Arg Asp 20 25 30 Ile Leu Ile Ile Arg Arg Asp Ile Gln Trp Glu Ile Gly Leu Ser Leu 35 40 45 Lys His Asn His Phe Ala Val Lys His Ser Arg Leu Ser Arg Lys Leu 50 55 60 Asp Phe Gly Asn Glu Trp Tyr Gly Ile Ser Cys Ser Glu Ala Tyr Trp 65 70 75 80 Lys Gly Val Asn Pro Val Phe Asp Tyr Leu Val Val Glu Lys Ser Lys 85 90 95 His Lys Lys Phe Asn Glu Leu Lys Asn Lys Glu Glu Val Val Tyr Val 100 105 110 Pro Leu Leu Lys Ala Phe Ile Asp Glu Ile Lys Gln Gln Cys Gln Val 115 120 125 His Lys Asp Ile Pro Ser Lys Leu Val Gln Tyr Leu Leu Gly Lys Tyr 130 135 140 Asp Phe Tyr Lys Ile Ile Ser Ile Asp Lys Glu Arg Met Thr Gln Ile 145 150 155 160 Gln Ser Tyr Asn Leu His Gly Thr Leu Asn Lys Asn Ser Glu Ser Lys 165 170 175 Lys Ala Ser Ile Arg Ile Pro Leu Ala Ser Leu Pro Thr Arg Ile Val 180 185 190 Ser Leu Asp Phe Val Pro Gly Lys Thr Asn Thr Val Glu Leu Tyr Met 195 200 205 Asp Gly Gly Trp Gln Phe Ser Phe Arg Ile His Asn Ala Glu Thr Tyr 210 215 220 Val Ala Pro Thr Leu Lys Phe Asp Ile Gln Ile Val Gly Met Pro Thr 225 230 235 240 Ala Ile Ile Thr Ile Asn Cys Leu Trp Lys 245 250 93660DNAFrankia species Eul1b 93atgaccgacg agttgtttcc tatcccggag ccattggtca gaccagtcat cgcactcccc 60cctcatctga aggaattgat cgatctactc ccattgaata cgccggtaca tcgccgagat 120ctcgaagcga agtatgggcg ctccaactat gctagacgca tacgaaagat tatcagtgaa 180tacggttggg aaatcgagag tcgccgccag tcggaaggcg ccaatgacga ttggtacatc 240cgtcggtccg acggccccgt gcgaccgcag cgtattagac gggaggtacc aagacgcagc 300cgcgagaccg tctacagacg tgacgactgg atctgccaga tttgtcggat gaaaaccgac 360ccggagcgtg gatctctcgt tccgcagtgc gatcacaaga ttccggcgga ccgcggaggg 420gattctgatg aagaaaatct tcagacgctt tgcacgcgtt gcaatctcaa gaagaggcag 480gcctgcggtg gatgcgctct ggccagctgt gcggattgtc catttgcgta tccagaaaag 540tttgatgatg tgctgattct gcacctcgac agggagcacc ttaagaggat tatgaccacg 600gcatacgctc gaaatgtcac ggccagtgca gtcgtcagcg acctatccga cctgctctag 66094219PRTFrankia species Eul1b 94Met Thr Asp Glu Leu Phe Pro Ile Pro Glu Pro Leu Val Arg Pro Val 1 5 10 15 Ile Ala Leu Pro Pro His Leu Lys Glu Leu Ile Asp Leu Leu Pro Leu 20 25 30 Asn Thr Pro Val His Arg Arg Asp Leu Glu Ala Lys Tyr Gly Arg Ser 35 40 45 Asn Tyr Ala Arg Arg Ile Arg Lys Ile Ile Ser Glu Tyr Gly Trp Glu 50 55 60 Ile Glu Ser Arg Arg Gln Ser Glu Gly Ala Asn Asp Asp Trp Tyr Ile 65 70 75 80 Arg Arg Ser Asp Gly Pro Val Arg Pro Gln Arg Ile Arg Arg Glu Val 85 90 95 Pro Arg Arg Ser Arg Glu Thr Val Tyr Arg Arg Asp Asp Trp Ile Cys 100 105 110 Gln Ile Cys Arg Met Lys Thr Asp Pro Glu Arg Gly Ser Leu Val Pro 115 120 125 Gln Cys Asp His Lys Ile Pro Ala Asp Arg Gly Gly Asp Ser Asp Glu 130 135 140 Glu Asn Leu Gln Thr Leu Cys Thr Arg Cys Asn Leu Lys Lys Arg Gln 145 150 155 160 Ala Cys Gly Gly Cys Ala Leu Ala Ser Cys Ala Asp Cys Pro Phe Ala 165 170 175 Tyr Pro Glu Lys Phe Asp Asp Val Leu Ile Leu His Leu Asp Arg Glu 180 185 190 His Leu Lys Arg Ile Met Thr Thr Ala Tyr Ala Arg Asn Val Thr Ala 195 200 205 Ser Ala Val Val Ser Asp Leu Ser Asp Leu Leu 210 215 95750DNAFischerella species 95gtgttgacaa acaacgagat tgaaagatta aggcaagcca ttatcgcgac cattgcatct 60cctgtaattg gctcgataga agattataca tgggaagcaa tttttcatta tgttaaggat 120attcctttat cagatcccgc tctgggacgc agcaagcttc tctatgatgc tgttgacgta 180gttactaaaa ctggttggtc actcaaatcc ctccaattga agagccttaa ctttaaaagc 240ccatttttat ttgttattca gagagcagat atccttaaga agtctgtcca gctgggtttt 300cctggtctga ctgagcaatc ttcgccggat gagcttggag cagccattat ccaacattgg 360aatgagaaga ttattttgag tcaggcagca caaagcgttg taaatagtta tgaaggcata 420ttactgaaaa ctatcaaagg ttacgagtat atctattgtg agtttccact cgatcctctt 480gatccaagca cgttttcttg ggcttggacg gtggacaaaa ctactggcgg tgcaggggta 540gggctacaag gtagcattgt gggcaaaaca gaattagtgt ggtataaaaa tcagaaacaa 600cttttcagag ctaggactat tcccgcacaa gcggttcgta ttacagttga aagaactcgt 660cttactcttg atcgatatgt aaagacagtt atctttgctt tgcaagatca aatcaacatg 720cagttttctg agaatgagcc tgaagaatag 75096249PRTFischerella species 96Met Leu Thr Asn Asn Glu Ile Glu Arg Leu Arg Gln Ala Ile Ile Ala 1 5 10 15 Thr Ile Ala Ser Pro Val Ile Gly Ser Ile Glu Asp Tyr Thr Trp Glu 20 25 30 Ala Ile Phe His Tyr Val Lys Asp Ile Pro Leu Ser Asp Pro Ala Leu 35 40 45 Gly Arg Ser Lys Leu Leu Tyr Asp Ala Val Asp Val Val Thr Lys Thr 50 55 60 Gly Trp Ser Leu Lys Ser Leu Gln Leu Lys Ser Leu Asn Phe Lys Ser 65 70 75 80 Pro Phe Leu Phe Val Ile Gln Arg Ala Asp Ile Leu Lys Lys Ser Val 85 90 95 Gln Leu Gly Phe Pro Gly Leu Thr Glu Gln Ser Ser Pro Asp Glu Leu 100 105 110 Gly Ala Ala Ile Ile Gln His Trp Asn Glu Lys Ile Ile Leu Ser Gln 115 120 125 Ala Ala Gln Ser Val Val Asn Ser Tyr Glu Gly Ile Leu Leu Lys Thr 130 135 140 Ile Lys Gly Tyr Glu Tyr Ile Tyr Cys Glu Phe Pro Leu Asp Pro Leu 145 150 155 160 Asp Pro Ser Thr Phe Ser Trp Ala Trp Thr Val Asp Lys Thr Thr Gly 165 170 175 Gly Ala Gly Val Gly Leu Gln Gly Ser Ile Val Gly Lys Thr Glu Leu 180 185 190 Val Trp Tyr Lys Asn Gln Lys Gln Leu Phe Arg Ala Arg Thr Ile Pro 195 200 205 Ala Gln Ala Val Arg Ile Thr Val Glu Arg Thr Arg Leu Thr Leu Asp 210 215 220 Arg Tyr Val Lys Thr Val Ile Phe Ala Leu Gln Asp Gln Ile Asn Met 225 230 235 240 Gln Phe Ser Glu Asn Glu Pro Glu Glu 245 97717DNAHaemophilus haemolyticus 97atgaattgga aagaatttga agttttttgt gttacttatt taaataaaac ttatggaaac 60aaatttgcga aaaaaggcga aagtgattct acaacaagtg atattctttt tacaggaaat 120aatccattct atatagaagc aaaaatgcca cattcccaat gcggtcaatt tgttttaatt 180cctaatagag ctaaatataa atttgattat tcaccgaaaa ataagagtga aataaatccc 240tatactcaaa aaataatgca atttatgtca gaaaacttct ctgaatatgc taatttatct 300actaaaggga aaattattcc attacctgaa tctgtatttg taaattggat taaggaatat 360tataaaagta aaagtgtgaa attctttatt acttctaatg gtgattttat tatatttcct 420attgaacact tcgagcatta ctttaacgta tcttgtacat acagaattaa aaaaagcggt 480ccaagacatc tcaattcgaa aagccttcct gatttcaaac aggcgttaga taaaaaaggc 540atctcttata cgatgagggg gttggaactg cattctgacg agaacattca cgataaaaga 600atttcaggag atgataagga ttttttaatt aaagagaata atggagctta tcacgttaag 660attttatcta atacttttaa tgctaatgtt ataattttca atatcattaa aaaataa 71798238PRTHaemophilus haemolyticus 98Met Asn Trp Lys Glu Phe Glu Val Phe Cys Val Thr Tyr Leu Asn Lys 1 5 10 15 Thr Tyr Gly Asn Lys Phe Ala Lys Lys Gly Glu Ser Asp Ser Thr Thr 20 25 30 Ser Asp Ile Leu Phe Thr Gly Asn Asn Pro Phe Tyr Ile Glu Ala Lys 35 40 45 Met Pro His Ser Gln Cys Gly Gln Phe Val Leu Ile Pro Asn Arg Ala 50 55 60 Lys Tyr Lys Phe Asp Tyr Ser Pro Lys Asn Lys Ser Glu Ile Asn Pro 65 70 75 80 Tyr Thr Gln Lys Ile Met Gln Phe Met Ser Glu Asn Phe Ser Glu Tyr 85 90 95 Ala Asn Leu Ser Thr Lys Gly Lys Ile Ile Pro Leu Pro Glu Ser Val 100 105 110 Phe Val Asn Trp Ile Lys Glu Tyr Tyr Lys Ser Lys Ser Val Lys Phe 115 120 125 Phe Ile Thr Ser Asn Gly Asp Phe Ile Ile Phe Pro Ile Glu His Phe 130 135 140 Glu His Tyr Phe Asn Val Ser Cys Thr Tyr Arg Ile Lys Lys Ser Gly 145 150 155 160 Pro Arg His Leu Asn Ser Lys Ser Leu Pro Asp Phe Lys Gln Ala Leu 165 170 175 Asp Lys Lys Gly Ile Ser Tyr Thr Met Arg Gly Leu Glu Leu His Ser 180 185 190 Asp Glu Asn Ile His Asp Lys Arg Ile Ser Gly Asp Asp Lys Asp Phe 195 200 205 Leu Ile Lys Glu Asn Asn Gly Ala Tyr His Val Lys Ile Leu Ser Asn 210 215 220 Thr Phe Asn Ala Asn Val Ile Ile Phe Asn Ile Ile Lys Lys 225 230 235 99744DNAHaemophilus influenzae P1 99atgaatctgg tagaattagg atctaaaaca gctaaagatg gttttaaaaa cgaaaaagat 60attgcagata gatttgaaaa ttggaaagag aattcagaag cccaagattg gttagttaca 120atgggacata acttagatga aatcaaatct gttaaagctg ttgtattaag tggatataaa 180tcagatataa atgttcaagt tttagttttt tataaagacg cgttagatat tcataatatt 240caagttaagc tcgttagtaa taaacgtggt tttaatcaga tagataaaca ctggcttgct 300cattatcagg aaatgtggaa atttgatgat aatctattaa gaatattaag acattttacg 360ggtgaacttc ctccatatca ttcaaataca aaagataagc gaagaatgtt tatgacagaa 420ttttcccaag aagagcaaaa tatcgttctt aattggttag aaaagaacag agttcttgtg 480ctaaccgata tattaagagg aagaggcgat tttgccgctg aatgggtgct tgtagcacaa 540aaagtaagta ataatgcaag atggatattg agaaatatta atgaggtttt acaacactat 600ggttcaggcg atatttctct ttccccaaga ggctctatta actttggtcg agtaactatt 660caaagaaaag ggggcgataa tggtagagaa accgcaaata tgttgcaatt caaaattgat 720ccaacagagt tatttgatat ttag 744100247PRTHaemophilus influenzae P1 100Met Asn Leu Val Glu Leu Gly Ser Lys Thr Ala Lys Asp Gly Phe Lys 1 5 10 15 Asn Glu Lys Asp Ile Ala Asp Arg Phe Glu Asn Trp Lys Glu Asn Ser 20 25 30 Glu Ala Gln Asp Trp Leu Val Thr Met Gly His Asn Leu Asp Glu Ile 35 40 45 Lys Ser Val Lys Ala Val Val Leu Ser Gly Tyr Lys Ser Asp Ile Asn 50 55 60 Val Gln Val Leu Val Phe Tyr Lys Asp Ala Leu Asp Ile His Asn Ile 65 70 75 80 Gln Val Lys Leu Val Ser Asn Lys Arg Gly Phe Asn Gln Ile Asp Lys 85 90 95 His Trp Leu Ala His Tyr Gln Glu Met Trp Lys Phe Asp Asp Asn Leu 100 105 110 Leu Arg Ile Leu Arg His Phe Thr Gly Glu Leu Pro Pro Tyr His Ser 115 120 125 Asn Thr Lys Asp Lys Arg Arg Met Phe Met Thr Glu Phe Ser Gln Glu 130 135 140 Glu Gln Asn Ile Val Leu Asn Trp Leu Glu Lys Asn Arg Val Leu Val 145 150 155 160 Leu Thr Asp Ile Leu Arg Gly Arg Gly Asp Phe Ala Ala Glu Trp Val 165 170 175 Leu Val Ala Gln Lys Val Ser Asn Asn Ala Arg Trp Ile Leu Arg Asn 180 185 190 Ile Asn Glu Val Leu Gln His Tyr Gly Ser Gly Asp Ile Ser Leu Ser 195 200 205 Pro Arg Gly Ser Ile Asn Phe

Gly Arg Val Thr Ile Gln Arg Lys Gly 210 215 220 Gly Asp Asn Gly Arg Glu Thr Ala Asn Met Leu Gln Phe Lys Ile Asp 225 230 235 240 Pro Thr Glu Leu Phe Asp Ile 245 101609DNAMycoplasma fermentans 101atgggtaaat ctgaattaag tggaagatta aattggcaag cattggctgg attaaaagct 60agtggtgctg aacaaaactt atataacgtg tttaacgctg tttttgaagg aactaaatac 120gttttatacg agaagccaaa gcaccttaaa aatctatacg ctcaagtagt cttacctgat 180gatgttatta aagaaatttt taatccttta attgatttat caactactca atggggtgtt 240tctccagatt tcgcaataga aaatacagaa acgcataaaa ttctttttgg tgaaattaaa 300agacaagatg gatgggtaga aggtaaagat cctagtgctg gcaggggtaa tgcacatgag 360agatcttgta aattatttac tcctggatta ttaaaagctt atagaacaat tggtggaatt 420aacgatgaag agatattgcc attctgggtt gtattcgaag gtgatataac acgagatccc 480aaaagagtaa gagaaattac tttctggtat gaccactatc aagataatta tttcatgtgg 540cgaccaaatg aatcaggcga aaaattagtt caacacttca atgaaaaatt aaaaaaatat 600ttagattaa 609102202PRTMycoplasma fermentans 102Met Gly Lys Ser Glu Leu Ser Gly Arg Leu Asn Trp Gln Ala Leu Ala 1 5 10 15 Gly Leu Lys Ala Ser Gly Ala Glu Gln Asn Leu Tyr Asn Val Phe Asn 20 25 30 Ala Val Phe Glu Gly Thr Lys Tyr Val Leu Tyr Glu Lys Pro Lys His 35 40 45 Leu Lys Asn Leu Tyr Ala Gln Val Val Leu Pro Asp Asp Val Ile Lys 50 55 60 Glu Ile Phe Asn Pro Leu Ile Asp Leu Ser Thr Thr Gln Trp Gly Val 65 70 75 80 Ser Pro Asp Phe Ala Ile Glu Asn Thr Glu Thr His Lys Ile Leu Phe 85 90 95 Gly Glu Ile Lys Arg Gln Asp Gly Trp Val Glu Gly Lys Asp Pro Ser 100 105 110 Ala Gly Arg Gly Asn Ala His Glu Arg Ser Cys Lys Leu Phe Thr Pro 115 120 125 Gly Leu Leu Lys Ala Tyr Arg Thr Ile Gly Gly Ile Asn Asp Glu Glu 130 135 140 Ile Leu Pro Phe Trp Val Val Phe Glu Gly Asp Ile Thr Arg Asp Pro 145 150 155 160 Lys Arg Val Arg Glu Ile Thr Phe Trp Tyr Asp His Tyr Gln Asp Asn 165 170 175 Tyr Phe Met Trp Arg Pro Asn Glu Ser Gly Glu Lys Leu Val Gln His 180 185 190 Phe Asn Glu Lys Leu Lys Lys Tyr Leu Asp 195 200 103828DNAMicrococcus luteus 103gtgagcgctc ccgaagtcga cagcgcccgg gatgcccgct acgtcgagat cctcctggct 60ccccttcgaa agtgtgggac ctacctgccg aagatgggcg ggtccggcga agtggatctc 120gctggcttca ccgcggccta cggggctgat ccgctctatc actggatggg gctcgactcg 180cctctcatgt tcgctgcgca caaggccgcc ggcggtatga cctcgatcta ccgccagctc 240ggtatcggat ccgagcgcct cttccgccag gtcctgcggg acgagctcaa tctcacagcc 300gaccaggtca agtggtccta caagatgctg cccgagcttg atgcggagca cgcgaacgag 360tcggtcaaag ctcgagtcct ctcgttggac gggagggtgg agctcgagga tctggaggat 420cagcaggctc gcgagcgcgt cgaagcttgg atagaagtac agcgccgtcg tctcaacatc 480accgcacccc tcaagggcgc cgtcttcgag gttcgccaag ggtacaagtc agctgacagc 540aagcggcaga acgccgacct cgccaacgcg gcgcaagccc tcgggcacca gtaccttccg 600gtgctcgtca tcatgtccac ccagatcaac gaggtcgtcc acgcccgcta cacgacgggc 660aactggtccg tactcatggg cacggttggg gcctcggacc cggtgggcag tacctacgac 720ttccttgatc aggtcgtagg ttacgaccta gccgcgttct tcgagcgcaa caaggctctc 780cgcgctggca ccgagggcat tctcactgat cttctggagg cccggtga 828104276PRTMicrococcus luteus 104Met Ser Ala Pro Glu Val Asp Ser Ala Arg Asp Ala Arg Tyr Val Glu 1 5 10 15 Ile Leu Leu Ala Pro Leu Arg Lys Cys Gly Thr Tyr Leu Pro Lys Met 20 25 30 Gly Gly Ser Gly Glu Val Asp Leu Ala Gly Phe Thr Ala Ala Tyr Gly 35 40 45 Ala Asp Pro Leu Tyr His Trp Met Gly Leu Asp Ser Pro Leu Met Phe 50 55 60 Ala Ala His Lys Ala Ala Gly Gly Met Thr Ser Ile Tyr Arg Gln Leu 65 70 75 80 Gly Ile Gly Ser Glu Arg Leu Phe Arg Gln Val Leu Arg Asp Glu Leu 85 90 95 Asn Leu Thr Ala Asp Gln Val Lys Trp Ser Tyr Lys Met Leu Pro Glu 100 105 110 Leu Asp Ala Glu His Ala Asn Glu Ser Val Lys Ala Arg Val Leu Ser 115 120 125 Leu Asp Gly Arg Val Glu Leu Glu Asp Leu Glu Asp Gln Gln Ala Arg 130 135 140 Glu Arg Val Glu Ala Trp Ile Glu Val Gln Arg Arg Arg Leu Asn Ile 145 150 155 160 Thr Ala Pro Leu Lys Gly Ala Val Phe Glu Val Arg Gln Gly Tyr Lys 165 170 175 Ser Ala Asp Ser Lys Arg Gln Asn Ala Asp Leu Ala Asn Ala Ala Gln 180 185 190 Ala Leu Gly His Gln Tyr Leu Pro Val Leu Val Ile Met Ser Thr Gln 195 200 205 Ile Asn Glu Val Val His Ala Arg Tyr Thr Thr Gly Asn Trp Ser Val 210 215 220 Leu Met Gly Thr Val Gly Ala Ser Asp Pro Val Gly Ser Thr Tyr Asp 225 230 235 240 Phe Leu Asp Gln Val Val Gly Tyr Asp Leu Ala Ala Phe Phe Glu Arg 245 250 255 Asn Lys Ala Ala Leu Arg Ala Gly Thr Glu Gly Ile Leu Thr Asp Leu 260 265 270 Leu Glu Ala Arg 275 1051281DNAMethylophilus methylotrophus 105atgagtgaaa ctaatcttaa tcaattagct tggacatcgt tatttgaaaa atacgatatt 60ttcaatcaat tagaaacaca taatttcttt aatatcacct ctacacaaat aaaccaattt 120cgtgaagcaa ggttaatgac taagtttgat aatactagtc aacttcctaa tattttttct 180aaaaatggta ttggaatatt gccaacctct cgtggctctt acacattagg aaaatttaat 240attttccata aatttgaaga aataccagaa gaagtagagc attatagatt ttgcaatatt 300tatgaaagcc tagatttcaa taatattagt tcggagtcaa cagctataag ctgcgcttct 360atatcaaaaa tattagacga ttttattggt gaagaattag tttcaactgt ttcaggcaga 420atgggaacaa gcacttttga attcagttta gataaatttc atactaaaaa aatcacagtt 480gaaaaagcac aaattgaaat tgacggagga tatgaaggcg aaaaatcttt tgtattgatt 540gaagctaaaa actacatatc cgacgatttc attattagac agctttatta tccatttaga 600aaatggaaag aaacaattca aaaagaggta aaaaatgttt acctcactta ttcaaatgga 660gtatttgaat taagagagta tgcttttaca gatattgaag gctataactc tatctatctc 720gttaaaagta agaggtatgc tatttacaat attgtgatca atgttgaaat aatacagcaa 780ttaattttag ctactgccat agagccagag ccattagata cacctttccc acaagccgac 840tcttttgaaa gagtaatcaa gttatgtgaa ttgattaaca cttccgaaat attgagcaaa 900gacgaaatta cagaaaacta cgactttgac tctcggcaaa ctgattacta tttgaatgct 960tgcaaatatc taggcttaac agaaaaggca tttaaagacg gtggtatagc tgcctgtctc 1020agtagcaaag gaaaagcgat atttaaaaag gatattagct ctcgtagact tgattttatt 1080aagctgatat tggctaaaac cgtatttaga aaaacattag agttatattt caataaagcc 1140agcatgccca ccaaagacga agttgtattg ataatgaaag agtcaaaact aaataaagta 1200acttccgaag aaacatacag cagaagagct tctactgtat tgggctggac aaactggata 1260attaatcaaa tagaagaata g 1281106426PRTMethylophilus methylotrophus 106Met Ser Glu Thr Asn Leu Asn Gln Leu Ala Trp Thr Ser Leu Phe Glu 1 5 10 15 Lys Tyr Asp Ile Phe Asn Gln Leu Glu Thr His Asn Phe Phe Asn Ile 20 25 30 Thr Ser Thr Gln Ile Asn Gln Phe Arg Glu Ala Arg Leu Met Thr Lys 35 40 45 Phe Asp Asn Thr Ser Gln Leu Pro Asn Ile Phe Ser Lys Asn Gly Ile 50 55 60 Gly Ile Leu Pro Thr Ser Arg Gly Ser Tyr Thr Leu Gly Lys Phe Asn 65 70 75 80 Ile Phe His Lys Phe Glu Glu Ile Pro Glu Glu Val Glu His Tyr Arg 85 90 95 Phe Cys Asn Ile Tyr Glu Ser Leu Asp Phe Asn Asn Ile Ser Ser Glu 100 105 110 Ser Thr Ala Ile Ser Cys Ala Ser Ile Ser Lys Ile Leu Asp Asp Phe 115 120 125 Ile Gly Glu Glu Leu Val Ser Thr Val Ser Gly Arg Met Gly Thr Ser 130 135 140 Thr Phe Glu Phe Ser Leu Asp Lys Phe His Thr Lys Lys Ile Thr Val 145 150 155 160 Glu Lys Ala Gln Ile Glu Ile Asp Gly Gly Tyr Glu Gly Glu Lys Ser 165 170 175 Phe Val Leu Ile Glu Ala Lys Asn Tyr Ile Ser Asp Asp Phe Ile Ile 180 185 190 Arg Gln Leu Tyr Tyr Pro Phe Arg Lys Trp Lys Glu Thr Ile Gln Lys 195 200 205 Glu Val Lys Asn Val Tyr Leu Thr Tyr Ser Asn Gly Val Phe Glu Leu 210 215 220 Arg Glu Tyr Ala Phe Thr Asp Ile Glu Gly Tyr Asn Ser Ile Tyr Leu 225 230 235 240 Val Lys Ser Lys Arg Tyr Ala Ile Tyr Asn Ile Val Ile Asn Val Glu 245 250 255 Ile Ile Gln Gln Leu Ile Leu Ala Thr Ala Ile Glu Pro Glu Pro Leu 260 265 270 Asp Thr Pro Phe Pro Gln Ala Asp Ser Phe Glu Arg Val Ile Lys Leu 275 280 285 Cys Glu Leu Ile Asn Thr Ser Glu Ile Leu Ser Lys Asp Glu Ile Thr 290 295 300 Glu Asn Tyr Asp Phe Asp Ser Arg Gln Thr Asp Tyr Tyr Leu Asn Ala 305 310 315 320 Cys Lys Tyr Leu Gly Leu Thr Glu Lys Ala Phe Lys Asp Gly Gly Ile 325 330 335 Ala Ala Cys Leu Ser Ser Lys Gly Lys Ala Ile Phe Lys Lys Asp Ile 340 345 350 Ser Ser Arg Arg Leu Asp Phe Ile Lys Leu Ile Leu Ala Lys Thr Val 355 360 365 Phe Arg Lys Thr Leu Glu Leu Tyr Phe Asn Lys Ala Ser Met Pro Thr 370 375 380 Lys Asp Glu Val Val Leu Ile Met Lys Glu Ser Lys Leu Asn Lys Val 385 390 395 400 Thr Ser Glu Glu Thr Tyr Ser Arg Arg Ala Ser Thr Val Leu Gly Trp 405 410 415 Thr Asn Trp Ile Ile Asn Gln Ile Glu Glu 420 425 107786DNAMicrococcus species 107atgggcgaca tggcgtacag ggaccgaccg ctcaacgcag aggagatgga ggctctccgc 60ctcgtcctaa gcacgtaccg ggattcctcg ggacagaacc aaaccaaata cgggtctatg 120cctgggttcc gcgacttcga gcgaggtctg gcgagcgtgc tgggcggtac cgccgcggag 180aacaagggtg tcttcgacat catcgtcaca cctagcgacg gcagcacagc cttcggaatc 240tcgtgcaaga tggcccggtt cgcgccgaag gcacagaacg cggcgttcgt tgaactctcc 300aacgcggccg cgaagttccg ggcgcacctc ttggagcgcc agatcaactg ggccaccgac 360cccatgctcg caggaccggc gatcatcgag ttggtcacga agtggcacac cgatgacgcc 420aacgagcatg ggctggacct cgataagagc gcctacgccg tactaagccg gagcagtgat 480tggtccactt accagttgtc cactttcccg ctggacctct atggcttcaa cccgattggg 540gacatcgcgt ggacggcaac gaccaagcgc atagatgggc acgtggagat caacggccag 600ccccatctgc tatggcagtg gtaccccacc agtgggggtc agttgaagtg gtggcctccg 660ctctcctggg ctacgtggtc gactgagcct tttactttgg aggagccgcc gttggttcgc 720ccggtggaac gcgcggagga gtacttccca gacctgtggc ctcacggatt cactccttct 780gcttga 786108261PRTMicrococcus species 108Met Gly Asp Met Ala Tyr Arg Asp Arg Pro Leu Asn Ala Glu Glu Met 1 5 10 15 Glu Ala Leu Arg Leu Val Leu Ser Thr Tyr Arg Asp Ser Ser Gly Gln 20 25 30 Asn Gln Thr Lys Tyr Gly Ser Met Pro Gly Phe Arg Asp Phe Glu Arg 35 40 45 Gly Leu Ala Ser Val Leu Gly Gly Thr Ala Ala Glu Asn Lys Gly Val 50 55 60 Phe Asp Ile Ile Val Thr Pro Ser Asp Gly Ser Thr Ala Phe Gly Ile 65 70 75 80 Ser Cys Lys Met Ala Arg Phe Ala Pro Lys Ala Gln Asn Ala Ala Phe 85 90 95 Val Glu Leu Ser Asn Ala Ala Ala Lys Phe Arg Ala His Leu Leu Glu 100 105 110 Arg Gln Ile Asn Trp Ala Thr Asp Pro Met Leu Ala Gly Pro Ala Ile 115 120 125 Ile Glu Leu Val Thr Lys Trp His Thr Asp Asp Ala Asn Glu His Gly 130 135 140 Leu Asp Leu Asp Lys Ser Ala Tyr Ala Val Leu Ser Arg Ser Ser Asp 145 150 155 160 Trp Ser Thr Tyr Gln Leu Ser Thr Phe Pro Leu Asp Leu Tyr Gly Phe 165 170 175 Asn Pro Ile Gly Asp Ile Ala Trp Thr Ala Thr Thr Lys Arg Ile Asp 180 185 190 Gly His Val Glu Ile Asn Gly Gln Pro His Leu Leu Trp Gln Trp Tyr 195 200 205 Pro Thr Ser Gly Gly Gln Leu Lys Trp Trp Pro Pro Leu Ser Trp Ala 210 215 220 Thr Trp Ser Thr Glu Pro Phe Thr Leu Glu Glu Pro Pro Leu Val Arg 225 230 235 240 Pro Val Glu Arg Ala Glu Glu Tyr Phe Pro Asp Leu Trp Pro His Gly 245 250 255 Phe Thr Pro Ser Ala 260 1091107DNANeisseria denitrificans 109atgaaaaact tatctttctc acaattagac tcttttttta gaaaagatga ttttccaagt 60attgagagac atcaatatgg cattaggtat ttaaaattaa gaagtatgtc tcgtaaagaa 120attatggaag aattttttca ggaatatgaa attgacatct ctaagcttaa gtcaaaagaa 180tattttagat atgcttttga gaacattgat ataaccatag aaagtataaa tagttttatt 240gaaaagaaat accaaataga gcgtaccgat agacttctac aagaagatta tttagtagat 300caactgagca gattacaata cttcgattgg ggcggttcat ttggtaatag tcttgaaaaa 360aacattgtcg ataattatgt caaaaaaata caatcttttg acataattaa caaaaaaata 420gaaactgagc tattttcaag tttacaggga tacactctaa attcttggta taatcactgg 480acttcaattt taattgaaga tatttttaaa gatcatgcta atgtattgcc aaccatcgga 540cttataaaaa agattgattt ctttataaat gagatacctt ttgatttaaa ggttacttat 600tttcctgagc agttcttagc tgaaaaattg aagcaaaagg ggtttggcaa tgagttaact 660agattaaaac aaatatgtag aaaactgaac attttaattc ctaatgacat gtctgacaaa 720aacttaaaac tgcatttata cacaaaagtt tcagaatgtc atcataaaga agctaaagaa 780ttaataaatg aattaaataa gttaaaaaaa caaattattc gtgaagccga acaaaattca 840gatgaattaa aagtatggct ttatgaaaat cagggtgaag cccgttttga cgcttcaaat 900agattttttt taattctcac ggacgagacc aatatcaatg atagttggaa acttaaaaga 960aacattaaat tcctaagaga gaaaattcac tctcatctag attctataaa actggatctt 1020aataaactaa atacaaaatt ttactggaag aaaacaaatg aacattttaa ttgtaagtct 1080gatatacttt ttataaaaca gacttaa 1107110368PRTNeisseria denitrificanse 110Met Lys Asn Leu Ser Phe Ser Gln Leu Asp Ser Phe Phe Arg Lys Asp 1 5 10 15 Asp Phe Pro Ser Ile Glu Arg His Gln Tyr Gly Ile Arg Tyr Leu Lys 20 25 30 Leu Arg Ser Met Ser Arg Lys Glu Ile Met Glu Glu Phe Phe Gln Glu 35 40 45 Tyr Glu Ile Asp Ile Ser Lys Leu Lys Ser Lys Glu Tyr Phe Arg Tyr 50 55 60 Ala Phe Glu Asn Ile Asp Ile Thr Ile Glu Ser Ile Asn Ser Phe Ile 65 70 75 80 Glu Lys Lys Tyr Gln Ile Glu Arg Thr Asp Arg Leu Leu Gln Glu Asp 85 90 95 Tyr Leu Val Asp Gln Leu Ser Arg Leu Gln Tyr Phe Asp Trp Gly Gly 100 105 110 Ser Phe Gly Asn Ser Leu Glu Lys Asn Ile Val Asp Asn Tyr Val Lys 115 120 125 Lys Ile Gln Ser Phe Asp Ile Ile Asn Lys Lys Ile Glu Thr Glu Leu 130 135 140 Phe Ser Ser Leu Gln Gly Tyr Thr Leu Asn Ser Trp Tyr Asn His Trp 145 150 155 160 Thr Ser Ile Leu Ile Glu Asp Ile Phe Lys Asp His Ala Asn Val Leu 165 170 175 Pro Thr Ile Gly Leu Ile Lys Lys Ile Asp Phe Phe Ile Asn Glu Ile 180 185 190 Pro Phe Asp Leu Lys Val Thr Tyr Phe Pro Glu Gln Phe Leu Ala Glu 195 200 205 Lys Leu Lys Gln Lys Gly Phe Gly Asn Glu Leu Thr Arg Leu Lys Gln 210 215 220 Ile Cys Arg Lys Leu Asn Ile Leu Ile Pro Asn Asp Met Ser Asp Lys 225 230 235 240 Asn Leu Lys Leu His Leu Tyr Thr Lys Val Ser Glu Cys His His Lys 245 250 255 Glu Ala Lys Glu Leu Ile Asn Glu Leu Asn Lys Leu Lys Lys Gln Ile 260 265 270 Ile Arg Glu Ala Glu Gln Asn Ser Asp Glu Leu Lys Val Trp Leu Tyr 275 280 285 Glu Asn Gln Gly Glu Ala Arg Phe Asp Ala Ser Asn Arg Phe Phe Leu 290 295 300 Ile Leu Thr Asp Glu Thr Asn Ile Asn Asp Ser Trp Lys Leu Lys Arg 305 310 315 320 Asn Ile Lys Phe

Leu Arg Glu Lys Ile His Ser His Leu Asp Ser Ile 325 330 335 Lys Leu Asp Leu Asn Lys Leu Asn Thr Lys Phe Tyr Trp Lys Lys Thr 340 345 350 Asn Glu His Phe Asn Cys Lys Ser Asp Ile Leu Phe Ile Lys Gln Thr 355 360 365 1112940DNANeisseria gonorrhoeae MS11 111atgagcggtt ttaattacga gaaaaaccag ccgcaccaaa tgcgggcggt ttcggcggtt 60ttgggcgtgt ttgacggggc aacgcccaaa tatcggacgg cagacgaaaa tcccgaactt 120ttgtttgctg caaaacaata cgcaaacaat atcctgaaag tgcaaagcca aaacggtata 180gacggccgat tccccgaccg ttcggacgac caaaatatcc ttgatatttc catggaaacg 240ggcacgggca aaacctatac ctacacacaa accatgttcg agctgcaccg ttggctgggc 300gtgttcaaat ttatcgtggt cgtgccgact ttgtccatta aggcgggaac acagcagttt 360ttgcaaagca aggctttggc agagcatttt gaacaggatt tcggcggcga ttatgaaggc 420gtacgcctga aaacctatgt ggtggaaagc gcgaaaaaga ataagggcaa aaagtccaat 480gcgcccataa cgattgagca atttgtcaaa gcggaaaaca aaaaggaaat tcatgtgctg 540ctgattaacg cgggcatggt taattcgtcg tccatgaacg atacgggcga caaggcattg 600aaggatttgt ttgacaatcc cgttgatgca ttggctgccg tgcgcccgtt tatgattgtg 660gacgaaccgc ataaattccc gacccgagat agcgcgaaaa cgtggggcaa tatcaaacgc 720ttaaaaccgc aatatatttt gcgctacggt gcaacattta acgatgaata ttacaacttg 780ctttaccgtt tgacggcagt agacgcgttt aacgacgggc tggtcaaagg cgtgcgcgtg 840tttcaggaag aaatgcaggg cggcatggat gcggcggtaa aactggtgtc gtcggacggc 900aaagaagcga aatttgaatt aaacgaaaag gacaaaaagc agacgttcaa actggcaaaa 960ggcgaagatt tggcgcaaat ccatccggct atttcggatt tgaaaatcga caaaatgaat 1020aaaaccgtgg tggtgttaag caacggcttg gagttgaaaa cgggtgccgt catcaaccct 1080tattcctatt cgcaaacggt gcaggatgcg atgatgcagc gggcggttgc cgaacatttc 1140aagctggaac gcgcgctttt ggcagaacgc gcgctacagc ccaaaatcaa gccgctgacg 1200ctgtttttta ttgacgatat cgcgggctac cgcagcggca acgagctttc aggcagcctg 1260aaagataaat ttgaaagctg gattcgcgcg gaagccgcac gccgtctgaa aacggaaagc 1320gacccgtttt accgcgatta cctgcaaaag acgttggacg atgtatccgc ctgccacggc 1380ggctattttt ccaaagacaa tacagacagc gacgatagaa tcgagcagga aatcaatgaa 1440atcctgcacg ataaggaaaa actgctgtct ttggacaacc cgcgccgctt tattttttcc 1500aaatggacgc tgcgcgaagg ctgggacaat cccaacgttt tccagatttg caaactgcgt 1560tccagcggca gcacgacttc caagctgcaa gaagtcggac gcggcctgcg cctgccggta 1620aacgagctga tggcgcgggt gcgcgatgta ccgtacaaac tgaattattt tgtcgatagc 1680agcgaaaaag actttgtgaa gcagcttgtc ggcgaaatca acgacaattc ttttcaggaa 1740gaaatctcca aaaagtttac cgaagagctg aaacaaaaaa tattgcaaaa ataccccgat 1800atcaaaccgc tggtattggt aaaccaactg ttttcagacg gcatcattga cgacaatgaa 1860aactttgccg aagacggcta tgacaaatta aaagccgcct atcccgaagc cttccccaaa 1920ggtttggaca aaggcaaagt cagcaacgcc aaagacgagg gcaaagacac catcatcatg 1980cgcgaaggca aatatgaaga actcaaagcc ttgtgggagc tgattcacca taaagccgtt 2040ttgcagtaca aaatcaaaga tgaagccgaa tttgtcgatt tatttaccgc ctatttgcgt 2100gaaaacgccg ccaaattccc gcaggcaggc atatgcacgg cggtaaacga agcttatatc 2160aacaacgggc ttatgctttc ccgccgcata gacagtattg aagatgaaga ttttatccgt 2220ttcaacacaa tgacttaccg tgagtttctg gaaaaactgg cacaaacggc aaaaatccag 2280atgcagactt tgcatcaggc gttttaccgc gtccgcgacg aactgaacat tggcgatttt 2340ttgaatatgc agaccatcgc ccaaatcaaa aacggcttca accggttttt gcttcatcat 2400tccttccata aattcgaact ggattaccgg cttgtcggca gcaaaatcca tccgaccaaa 2460tttaccaata aagacggcaa accgcgcgcg gtgaaaaaag cagatttcgg cagatttgaa 2520gatacggagc accggcctgc cgccggctat ctcttcggcg agattttcta cgattcggat 2580atagaacatg aaaatgtcgc caacaaccaa attgaaggcg taatcgtatt taccaaaata 2640ccgagaaact ccatcaaaat ccctgttgcc ggcggcggca cgtattcgcc cgactttgcc 2700tatatcgtga aaaccaaaag cggcgagatt ctgaactttg tgattgaagc caaagggact 2760gacggggcgg aagatttgcg aaaaagcgaa gagcggaaaa tcaaacatgc cgaaaagctg 2820tttgccgaga tttccaaaga aatcaaggtg gtgttcaaaa cgcagtttga cggcgagagg 2880atagccgaac tgatcgggca aaatatgcca gcaggcgggc attctgaaaa cggacactga 2940112979PRTNeisseria gonorrhoeae MS11 112Met Ser Gly Phe Asn Tyr Glu Lys Asn Gln Pro His Gln Met Arg Ala 1 5 10 15 Val Ser Ala Val Leu Gly Val Phe Asp Gly Ala Thr Pro Lys Tyr Arg 20 25 30 Thr Ala Asp Glu Asn Pro Glu Leu Leu Phe Ala Ala Lys Gln Tyr Ala 35 40 45 Asn Asn Ile Leu Lys Val Gln Ser Gln Asn Gly Ile Asp Gly Arg Phe 50 55 60 Pro Asp Arg Ser Asp Asp Gln Asn Ile Leu Asp Ile Ser Met Glu Thr 65 70 75 80 Gly Thr Gly Lys Thr Tyr Thr Tyr Thr Gln Thr Met Phe Glu Leu His 85 90 95 Arg Trp Leu Gly Val Phe Lys Phe Ile Val Val Val Pro Thr Leu Ser 100 105 110 Ile Lys Ala Gly Thr Gln Gln Phe Leu Gln Ser Lys Ala Leu Ala Glu 115 120 125 His Phe Glu Gln Asp Phe Gly Gly Asp Tyr Glu Gly Val Arg Leu Lys 130 135 140 Thr Tyr Val Val Glu Ser Ala Lys Lys Asn Lys Gly Lys Lys Ser Asn 145 150 155 160 Ala Pro Ile Thr Ile Glu Gln Phe Val Lys Ala Glu Asn Lys Lys Glu 165 170 175 Ile His Val Leu Leu Ile Asn Ala Gly Met Val Asn Ser Ser Ser Met 180 185 190 Asn Asp Thr Gly Asp Lys Ala Leu Lys Asp Leu Phe Asp Asn Pro Val 195 200 205 Asp Ala Leu Ala Ala Val Arg Pro Phe Met Ile Val Asp Glu Pro His 210 215 220 Lys Phe Pro Thr Arg Asp Ser Ala Lys Thr Trp Gly Asn Ile Lys Arg 225 230 235 240 Leu Lys Pro Gln Tyr Ile Leu Arg Tyr Gly Ala Thr Phe Asn Asp Glu 245 250 255 Tyr Tyr Asn Leu Leu Tyr Arg Leu Thr Ala Val Asp Ala Phe Asn Asp 260 265 270 Gly Leu Val Lys Gly Val Arg Val Phe Gln Glu Glu Met Gln Gly Gly 275 280 285 Met Asp Ala Ala Val Lys Leu Val Ser Ser Asp Gly Lys Glu Ala Lys 290 295 300 Phe Glu Leu Asn Glu Lys Asp Lys Lys Gln Thr Phe Lys Leu Ala Lys 305 310 315 320 Gly Glu Asp Leu Ala Gln Ile His Pro Ala Ile Ser Asp Leu Lys Ile 325 330 335 Asp Lys Met Asn Lys Thr Val Val Val Leu Ser Asn Gly Leu Glu Leu 340 345 350 Lys Thr Gly Ala Val Ile Asn Pro Tyr Ser Tyr Ser Gln Thr Val Gln 355 360 365 Asp Ala Met Met Gln Arg Ala Val Ala Glu His Phe Lys Leu Glu Arg 370 375 380 Ala Leu Leu Ala Glu Arg Ala Leu Gln Pro Lys Ile Lys Pro Leu Thr 385 390 395 400 Leu Phe Phe Ile Asp Asp Ile Ala Gly Tyr Arg Ser Gly Asn Glu Leu 405 410 415 Ser Gly Ser Leu Lys Asp Lys Phe Glu Ser Trp Ile Arg Ala Glu Ala 420 425 430 Ala Arg Arg Leu Lys Thr Glu Ser Asp Pro Phe Tyr Arg Asp Tyr Leu 435 440 445 Gln Lys Thr Leu Asp Asp Val Ser Ala Cys His Gly Gly Tyr Phe Ser 450 455 460 Lys Asp Asn Thr Asp Ser Asp Asp Arg Ile Glu Gln Glu Ile Asn Glu 465 470 475 480 Ile Leu His Asp Lys Glu Lys Leu Leu Ser Leu Asp Asn Pro Arg Arg 485 490 495 Phe Ile Phe Ser Lys Trp Thr Leu Arg Glu Gly Trp Asp Asn Pro Asn 500 505 510 Val Phe Gln Ile Cys Lys Leu Arg Ser Ser Gly Ser Thr Thr Ser Lys 515 520 525 Leu Gln Glu Val Gly Arg Gly Leu Arg Leu Pro Val Asn Glu Leu Met 530 535 540 Ala Arg Val Arg Asp Val Pro Tyr Lys Leu Asn Tyr Phe Val Asp Ser 545 550 555 560 Ser Glu Lys Asp Phe Val Lys Gln Leu Val Gly Glu Ile Asn Asp Asn 565 570 575 Ser Phe Gln Glu Glu Ile Ser Lys Lys Phe Thr Glu Glu Leu Lys Gln 580 585 590 Lys Ile Leu Gln Lys Tyr Pro Asp Ile Lys Pro Leu Val Leu Val Asn 595 600 605 Gln Leu Phe Ser Asp Gly Ile Ile Asp Asp Asn Glu Asn Phe Ala Glu 610 615 620 Asp Gly Tyr Asp Lys Leu Lys Ala Ala Tyr Pro Glu Ala Phe Pro Lys 625 630 635 640 Gly Leu Asp Lys Gly Lys Val Ser Asn Ala Lys Asp Glu Gly Lys Asp 645 650 655 Thr Ile Ile Met Arg Glu Gly Lys Tyr Glu Glu Leu Lys Ala Leu Trp 660 665 670 Glu Leu Ile His His Lys Ala Val Leu Gln Tyr Lys Ile Lys Asp Glu 675 680 685 Ala Glu Phe Val Asp Leu Phe Thr Ala Tyr Leu Arg Glu Asn Ala Ala 690 695 700 Lys Phe Pro Gln Ala Gly Ile Cys Thr Ala Val Asn Glu Ala Tyr Ile 705 710 715 720 Asn Asn Gly Leu Met Leu Ser Arg Arg Ile Asp Ser Ile Glu Asp Glu 725 730 735 Asp Phe Ile Arg Phe Asn Thr Met Thr Tyr Arg Glu Phe Leu Glu Lys 740 745 750 Leu Ala Gln Thr Ala Lys Ile Gln Met Gln Thr Leu His Gln Ala Phe 755 760 765 Tyr Arg Val Arg Asp Glu Leu Asn Ile Gly Asp Phe Leu Asn Met Gln 770 775 780 Thr Ile Ala Gln Ile Lys Asn Gly Phe Asn Arg Phe Leu Leu His His 785 790 795 800 Ser Phe His Lys Phe Glu Leu Asp Tyr Arg Leu Val Gly Ser Lys Ile 805 810 815 His Pro Thr Lys Phe Thr Asn Lys Asp Gly Lys Pro Arg Ala Val Lys 820 825 830 Lys Ala Asp Phe Gly Arg Phe Glu Asp Thr Glu His Arg Pro Ala Ala 835 840 845 Gly Tyr Leu Phe Gly Glu Ile Phe Tyr Asp Ser Asp Ile Glu His Glu 850 855 860 Asn Val Ala Asn Asn Gln Ile Glu Gly Val Ile Val Phe Thr Lys Ile 865 870 875 880 Pro Arg Asn Ser Ile Lys Ile Pro Val Ala Gly Gly Gly Thr Tyr Ser 885 890 895 Pro Asp Phe Ala Tyr Ile Val Lys Thr Lys Ser Gly Glu Ile Leu Asn 900 905 910 Phe Val Ile Glu Ala Lys Gly Thr Asp Gly Ala Glu Asp Leu Arg Lys 915 920 925 Ser Glu Glu Arg Lys Ile Lys His Ala Glu Lys Leu Phe Ala Glu Ile 930 935 940 Ser Lys Glu Ile Lys Val Val Phe Lys Thr Gln Phe Asp Gly Glu Arg 945 950 955 960 Ile Ala Glu Leu Ile Gly Gln Asn Met Pro Ala Gly Gly His Ser Glu 965 970 975 Asn Gly His 1131152DNANocardia otitidis-caviarum 113atgcggtcag atacgtcggt ggagccagag ggcgccaact tcatcgcgga atttttcggg 60catcgtgtgt accccgaagt cgtcagcact gaagctgcga ggaatgacca ggcgacgggg 120acctgccctt tcttgacggc tgccaagctg gttgaaactt catgcgtaaa ggccgagacc 180tcgcgcgggg tttgcgtggt caacacagct gtagacaacg agcgctacga ctggttggtg 240tgtcccaacc gagcgttaga ccccctgttc atgtccgcag cttcgaggaa gctttttggc 300tacggaccca cagaaccgct tcagttcatc gcggcgccga cgttagccga tcaggcggtg 360cgcgacggaa tccgggaatg gctggatcgt ggagtccacg tggtcgctta cttccaggag 420aaactcggtg gcgagctgag catcagcaag accgatagct cgccggagtt ttcattcgac 480tggactcttg ccgaagtcga gtctatctac cccgtgccga agatcaagcg gtacggggtc 540cttgagatcc agactatgga cttccacggc tcgtacaagc atgctgtcgg tgctatcgac 600attgccttag tggagggaat tgatttccac ggctggttgc ccacaccagc gggtcgtgcc 660gctctctcga agaagatgga gggcccaaac ctctccaatg tgttcaagcg cacgttctac 720cagatggcat acaaattcgc tctgagtggt catcaacgat gtgccgggac cgggttcgcg 780attccgcaga gtgtctggaa aagctggctg agacatctgg ccaacccaac gctgatagac 840aacggggatg gcaccttctc tctgggggat acccggaatg atagtgaaaa cgcttggata 900ttcgtattcg aactagatcc ggatactgat gcctcgccgc gcccattggc gccccacctt 960gagattcgag tgaacgtgga cacgttgatt gatctcgcgc tgagagaatc gcccagggct 1020gctcttggcc cgtctgggcc ggtggctacg ttcaccgaca aggtcgaggc gcggatgtta 1080aggttctggc cgaagactcg ccgccgtcgc tcgacgacac caggggggca gcgggggctg 1140ttcgatgcat ga 1152114383PRTNocardia otitidis-caviarum 114Met Arg Ser Asp Thr Ser Val Glu Pro Glu Gly Ala Asn Phe Ile Ala 1 5 10 15 Glu Phe Phe Gly His Arg Val Tyr Pro Glu Val Val Ser Thr Glu Ala 20 25 30 Ala Arg Asn Asp Gln Ala Thr Gly Thr Cys Pro Phe Leu Thr Ala Ala 35 40 45 Lys Leu Val Glu Thr Ser Cys Val Lys Ala Glu Thr Ser Arg Gly Val 50 55 60 Cys Val Val Asn Thr Ala Val Asp Asn Glu Arg Tyr Asp Trp Leu Val 65 70 75 80 Cys Pro Asn Arg Ala Leu Asp Pro Leu Phe Met Ser Ala Ala Ser Arg 85 90 95 Lys Leu Phe Gly Tyr Gly Pro Thr Glu Pro Leu Gln Phe Ile Ala Ala 100 105 110 Pro Thr Leu Ala Asp Gln Ala Val Arg Asp Gly Ile Arg Glu Trp Leu 115 120 125 Asp Arg Gly Val His Val Val Ala Tyr Phe Gln Glu Lys Leu Gly Gly 130 135 140 Glu Leu Ser Ile Ser Lys Thr Asp Ser Ser Pro Glu Phe Ser Phe Asp 145 150 155 160 Trp Thr Leu Ala Glu Val Glu Ser Ile Tyr Pro Val Pro Lys Ile Lys 165 170 175 Arg Tyr Gly Val Leu Glu Ile Gln Thr Met Asp Phe His Gly Ser Tyr 180 185 190 Lys His Ala Val Gly Ala Ile Asp Ile Ala Leu Val Glu Gly Ile Asp 195 200 205 Phe His Gly Trp Leu Pro Thr Pro Ala Gly Arg Ala Ala Leu Ser Lys 210 215 220 Lys Met Glu Gly Pro Asn Leu Ser Asn Val Phe Lys Arg Thr Phe Tyr 225 230 235 240 Gln Met Ala Tyr Lys Phe Ala Leu Ser Gly His Gln Arg Cys Ala Gly 245 250 255 Thr Gly Phe Ala Ile Pro Gln Ser Val Trp Lys Ser Trp Leu Arg His 260 265 270 Leu Ala Asn Pro Thr Leu Ile Asp Asn Gly Asp Gly Thr Phe Ser Leu 275 280 285 Gly Asp Thr Arg Asn Asp Ser Glu Asn Ala Trp Ile Phe Val Phe Glu 290 295 300 Leu Asp Pro Asp Thr Asp Ala Ser Pro Arg Pro Leu Ala Pro His Leu 305 310 315 320 Glu Ile Arg Val Asn Val Asp Thr Leu Ile Asp Leu Ala Leu Arg Glu 325 330 335 Ser Pro Arg Ala Ala Leu Gly Pro Ser Gly Pro Val Ala Thr Phe Thr 340 345 350 Asp Lys Val Glu Ala Arg Met Leu Arg Phe Trp Pro Lys Thr Arg Arg 355 360 365 Arg Arg Ser Thr Thr Pro Gly Gly Gln Arg Gly Leu Phe Asp Ala 370 375 380 115429DNAPseudomonas alcaligenes 115atgacgcaat gtccaaggtg ccagcgcaat ctcgcagctg acgagttcta tgctggctct 60agcaaaatgt gcaagggttg catgacttgg caaaacctaa gctacaacgc gaataaggaa 120ggtcatgcca acaccttcac caaagcgaca tttttggcgt ggtacggctt atcagcacag 180cggcattgtg ggtattgcgg tatatcggag gcaggtttta catccttgca caggactaat 240ccacgcggct accacataca gtgtttgggt gttgatcgct cagattcgtt cgaaggctat 300tcacctcaaa acgctcggct cgcctgtttt atatgcaaca ggataaaatc aaacatcttc 360agcgccagtg agatggacgt tctaggtgag gccatttcaa aagcgtggca tggtcgagga 420attgcctaa 429116142PRTPseudomonas alcaligenes 116Met Thr Gln Cys Pro Arg Cys Gln Arg Asn Leu Ala Ala Asp Glu Phe 1 5 10 15 Tyr Ala Gly Ser Ser Lys Met Cys Lys Gly Cys Met Thr Trp Gln Asn 20 25 30 Leu Ser Tyr Asn Ala Asn Lys Glu Gly His Ala Asn Thr Phe Thr Lys 35 40 45 Ala Thr Phe Leu Ala Trp Tyr Gly Leu Ser Ala Gln Arg His Cys Gly 50 55 60 Tyr Cys Gly Ile Ser Glu Ala Gly Phe Thr Ser Leu His Arg Thr Asn 65 70 75 80 Pro Arg Gly Tyr His Ile Gln Cys Leu Gly Val Asp Arg Ser Asp Ser 85 90 95 Phe Glu Gly Tyr Ser Pro Gln Asn Ala Arg Leu Ala Cys Phe Ile Cys 100 105 110 Asn Arg Ile Lys Ser Asn Ile Phe Ser Ala Ser Glu Met Asp Val Leu 115 120 125 Gly Glu Ala Ile Ser Lys Ala Trp His Gly Arg Gly Ile Ala 130 135 140 117744DNAPseudomonas fluorescens 117atgcgtggac tggagatcgg tgtaaacgca ttagtttttt atcagacacg cactgagtgg 60aacgtaaata atcctgaaaa

cttgggatgg gagcccgcgg agaacaggat aagccctcta 120ggtgggcagt atgttgcgcg tatcgccgca accactgcgt tagataatgg cgaaaaaatt 180atccgtggtt tcacgacatc taaagtaaaa ggcgctggaa ttcggttatt tgaatacgcg 240ggagaaaagg atattagggc gtgtcgtctc cagcttgagg ctcttttttg gatgtgccgc 300gactcaacgg aagttgcgat aattaacggt atgactgctc aggacgcgtt gtctagaagt 360acctataacg cagctgagtg ccaaaaatat gatttgcttg atttaaatcg acttcatgaa 420gcgcgcatca taaatacaga tggtagaacc atctgtcctc tctgtcttga agagctttct 480ggtgaaggct ttttgagtcg gttggagcaa gcagaaggtc gagaggtaca cgaccttact 540gttacaaagt taaacttgtt tcatatttca gagctccgtt ttggagtcta taaccataag 600ccatacaacc taggttgggg gcatcaccac tgtaacgtcg ttgttaaaga ctcggggata 660atcgagacat tacaatggat gtatgaggtg gttcatcgaa atatcaacga tggtcacttt 720gctcctgaga acaatccgaa ctga 744118247PRTPseudomonas fluorescens 118Met Arg Gly Leu Glu Ile Gly Val Asn Ala Leu Val Phe Tyr Gln Thr 1 5 10 15 Arg Thr Glu Trp Asn Val Asn Asn Pro Glu Asn Leu Gly Trp Glu Pro 20 25 30 Ala Glu Asn Arg Ile Ser Pro Leu Gly Gly Gln Tyr Val Ala Arg Ile 35 40 45 Ala Ala Thr Thr Ala Leu Asp Asn Gly Glu Lys Ile Ile Arg Gly Phe 50 55 60 Thr Thr Ser Lys Val Lys Gly Ala Gly Ile Arg Leu Phe Glu Tyr Ala 65 70 75 80 Gly Glu Lys Asp Ile Arg Ala Cys Arg Leu Gln Leu Glu Ala Leu Phe 85 90 95 Trp Met Cys Arg Asp Ser Thr Glu Val Ala Ile Ile Asn Gly Met Thr 100 105 110 Ala Gln Asp Ala Leu Ser Arg Ser Thr Tyr Asn Ala Ala Glu Cys Gln 115 120 125 Lys Tyr Asp Leu Leu Asp Leu Asn Arg Leu His Glu Ala Arg Ile Ile 130 135 140 Asn Thr Asp Gly Arg Thr Ile Cys Pro Leu Cys Leu Glu Glu Leu Ser 145 150 155 160 Gly Glu Gly Phe Leu Ser Arg Leu Glu Gln Ala Glu Gly Arg Glu Val 165 170 175 His Asp Leu Thr Val Thr Lys Leu Asn Leu Phe His Ile Ser Glu Leu 180 185 190 Arg Phe Gly Val Tyr Asn His Lys Pro Tyr Asn Leu Gly Trp Gly His 195 200 205 His His Cys Asn Val Val Val Lys Asp Ser Gly Ile Ile Glu Thr Leu 210 215 220 Gln Trp Met Tyr Glu Val Val His Arg Asn Ile Asn Asp Gly His Phe 225 230 235 240 Ala Pro Glu Asn Asn Pro Asn 245 119695DNAPseudomonas mendocinamisc_feature(504)..(504)y= t/u or c 119atgacaacaa actccccctc agacgtcggc atgatcgacg agtgtctgtc catcgtccga 60acgtcgcttg cacgatgttt ccaacagcag gccccaagca ttcaagcctc atggccactt 120tcaggacgcg ccgtatctga gattggaggc cgcctagtcg agagtttcgt tttagcacga 180ctcccgcatg aactgagcac cacgcctttt gacggccaga ttctatgtga aatacctgaa 240tccggcagag cgatggaaga cattgcggtg accttcatcg gcccacatgg aagggctcga 300ctactcatcg acgtcaaggg tcataacgaa taccgcacgg gatcgagacc caatttggct 360tcgatccgaa aatgtctgga actctatcgc agctcctcac ataccgttga tgagctcgtt 420gtcttcttct gccgttaccg cccatccgtc cacccggatc atcacgcaca agcggtcgaa 480tatcacgttc tgcccgagtc gttyaatgag cagggamttt tcctgcttcg tgccctgagc 540gaaagcaacc tggatccagc caatatcgga gtggcggcca gttgctgctt gccagggaaa 600acaacatacg gttagtgaat cgttcaaggt cggagttcgt tcaacttcta gagggtctcc 660agtcacgcct tcaacggggg cgaagtacgg tttga 695120231PRTPseudomonas mendocina 120Met Thr Thr Asn Ser Pro Ser Asp Val Gly Met Ile Asp Glu Cys Leu 1 5 10 15 Ser Ile Val Arg Thr Ser Leu Ala Arg Cys Phe Gln Gln Gln Ala Pro 20 25 30 Ser Ile Gln Ala Ser Trp Pro Leu Ser Gly Arg Ala Val Ser Glu Ile 35 40 45 Gly Gly Arg Leu Val Glu Ser Phe Val Leu Ala Arg Leu Pro His Glu 50 55 60 Leu Ser Thr Thr Pro Phe Asp Gly Gln Ile Leu Cys Glu Ile Pro Glu 65 70 75 80 Ser Gly Arg Ala Met Glu Asp Ile Ala Val Thr Phe Ile Gly Pro His 85 90 95 Gly Arg Ala Arg Leu Leu Ile Asp Val Lys Gly His Asn Glu Tyr Arg 100 105 110 Thr Gly Ser Arg Pro Asn Leu Ala Ser Ile Arg Lys Cys Leu Glu Leu 115 120 125 Tyr Arg Ser Ser Ser His Thr Val Asp Glu Leu Val Val Phe Phe Cys 130 135 140 Arg Tyr Arg Pro Ser Val His Pro Asp His His Ala Gln Ala Val Glu 145 150 155 160 Tyr His Val Leu Pro Glu Ser Phe Asn Glu Gln Gly Leu Phe Leu Leu 165 170 175 Arg Ala Leu Ser Glu Ser Asn Leu Asp Pro Ala Asn Ile Gly Ser Gly 180 185 190 Gly Gln Leu Leu Leu Ala Arg Glu Asn Asn Ile Arg Leu Val Asn Arg 195 200 205 Ser Arg Ser Glu Phe Val Gln Leu Leu Glu Gly Leu Gln Ser Arg Leu 210 215 220 Gln Arg Gly Arg Ser Thr Val 225 230 121888DNAPlesiomonas shigelloides 319-73 121atgtcgattt tagataatga aaaacaattg agaatattga acataattaa cgagggtgtt 60actcctgcca taataccaga gcttcattcg ctagttgatg acaggattac taacgaagaa 120atcgaattgc tacataagaa agtctctaca cttatcgggc tttcaatacc cgtgctaaat 180attccccgtg acattttaaa ggcttttgaa ccttcccaga ttggaacaat tgttggaaca 240gtcatggatg cttgtattcc tcagctagat tcaattattg aggattcaaa agttatagcg 300gatattggtt tgcaaaaaca cgaagggatt cttggggaaa gagaaggtta cccagattat 360aagactaatg atgggtacag gcttgagctc aaactactat atgttgatcc tgatgatgtt 420gagatgaaaa agccccctac accaagagag gcatctgcga gactgactca aaaagtaacc 480tataagaatg tcgatacaag caaagaccta ttaatggttg tcgcgtatca gtttcgtgaa 540acacatgacc aaatatattc gccaacaata attgatgttg gaattttccc agtaattgat 600tgtatcttgg caagagatgt tcgtctttct ttatcgcctg gtcgatggtt tggaaatttt 660gaaacacctg caatattgag caatgctggc aaaattaaaa attctaacgg cgatccacta 720aataagtccg tatacggaag aaaagaatcc gaaggcttgg acttcaatga agatacaaat 780gtaggaaagc tagcaagaaa accattaaaa accttgcaag aatttcttaa gaaaaataac 840actaagtatg ccagcagagg ggtctatcca tcagcctgga caatccga 888122296PRTPlesiomonas shigelloides 319-73 122Met Ser Ile Leu Asp Asn Glu Lys Gln Leu Arg Ile Leu Asn Ile Ile 1 5 10 15 Asn Glu Gly Val Thr Pro Ala Ile Ile Pro Glu Leu His Ser Leu Val 20 25 30 Asp Asp Arg Ile Thr Asn Glu Glu Ile Glu Leu Leu His Lys Lys Val 35 40 45 Ser Thr Leu Ile Gly Leu Ser Ile Pro Val Leu Asn Ile Pro Arg Asp 50 55 60 Ile Leu Lys Ala Phe Glu Pro Ser Gln Ile Gly Thr Ile Val Gly Thr 65 70 75 80 Val Met Asp Ala Cys Ile Pro Gln Leu Asp Ser Ile Ile Glu Asp Ser 85 90 95 Lys Val Ile Ala Asp Ile Gly Leu Gln Lys His Glu Gly Ile Leu Gly 100 105 110 Glu Arg Glu Gly Tyr Pro Asp Tyr Lys Thr Asn Asp Gly Tyr Arg Leu 115 120 125 Glu Leu Lys Leu Leu Tyr Val Asp Pro Asp Asp Val Glu Met Lys Lys 130 135 140 Pro Pro Thr Pro Arg Glu Ala Ser Ala Arg Leu Thr Gln Lys Val Thr 145 150 155 160 Tyr Lys Asn Val Asp Thr Ser Lys Asp Leu Leu Met Val Val Ala Tyr 165 170 175 Gln Phe Arg Glu Thr His Asp Gln Ile Tyr Ser Pro Thr Ile Ile Asp 180 185 190 Val Gly Ile Phe Pro Val Ile Asp Cys Ile Leu Ala Arg Asp Val Arg 195 200 205 Leu Ser Leu Ser Pro Gly Arg Trp Phe Gly Asn Phe Glu Thr Pro Ala 210 215 220 Ile Leu Ser Asn Ala Gly Lys Ile Lys Asn Ser Asn Gly Asp Pro Leu 225 230 235 240 Asn Lys Ser Val Tyr Gly Arg Lys Glu Ser Glu Gly Leu Asp Phe Asn 245 250 255 Glu Asp Thr Asn Val Gly Lys Leu Ala Arg Lys Pro Leu Lys Thr Leu 260 265 270 Gln Glu Phe Leu Lys Lys Asn Asn Thr Lys Tyr Ala Ser Arg Gly Val 275 280 285 Tyr Pro Ser Ala Trp Thr Ile Arg 290 295 1232859DNAProvidencia stuartii 164 123atgagccgaa gcaacgccat aaaaattgcc aataagatta gtgcacgact gtcactacgc 60gatccccaag atgaatcatt gcgtatctta tgcaacgtac ttgaacaatt cagtctcagt 120aaagatcccg atcttaatcg ctggattgaa ttgctaagcc aacagtaccc tacagtgaaa 180gggtttgaac gagcctttcc ttcattatgc ttcgcactgg ctactggtgt gggtaaaaca 240cgcttaatgg gtgcaatgat tacttggcta tatttaaccg gacgcagccg tcatttcttc 300atactatctc caaatttaac catctatgaa aaacttaaga tggatttttt acccggttca 360ccaaagtatg ttttccaagg tattcctgaa cttgcacaaa cacctccggt tctaatcact 420ggtgatgact atcaggaagg gcggggtgtt cgtctagatt atgcaattgc cgaaagcaaa 480acgggtgatc tttttgacaa tgaaaccgct ccacacatta atatcttcaa tatttccaaa 540ataaacgcac tggaaaatgc caaaggtgct gctaaatcta aggtcgctaa aattcgaaga 600atacaggaat acatcggaga atcttatttt agctacctag cgaatctacc tgatttagtt 660attttgatgg atgaagctca ccgttattat gccagcgcag gcgcacaggc acttaacgat 720ctgaatccag tattgggtat tgaattaact gccacgccga aaactgtggg agcaaacccg 780cgcgatttta aaaatattat ttatcactat cctctctcac gggcattaaa agatggatat 840gtaaaaatcc cggcggttgc cacacgtaaa gagttccgcg ccgcaaatta ctctgaagaa 900caactagaaa aaataaagtt ggaagacggt atccatcatc atgaatatgt gaaaacagag 960ctaaccagct tcgctaacaa taccggtaac aaattaatta aaccttttat gctagttgtt 1020gcacaggata ccgaccatgc agacagccta aaagtacgta ttgaacacga cgatttcttc 1080aacggtgcct acaaaggcaa agtaatcacc gttcattcga accaaacggg tgaagaatca 1140gaagagacta tgcagcgact tttggccgtt gagtatgata aagatacaga aatagtcatt 1200catgtcaata agttaaaaga gggttgggat gttaccaacc tgtatactat tgttccatta 1260cgtgcttctg cttctgaaat cctgactgag caaaccatag ggcgagggct acgcctaccg 1320tacggtaaaa gaacaggcgt cgaagctgtt gatcgtctga caatcattgc ccatgatcgt 1380tttcaagata ttatcgaccg tgccaataat gatgactcga ttattaaaaa agtcctttat 1440atagggttag atgatgatga aaatggtatt ccagaagtaa aacctcagca aattgtcgta 1500ccatcaatgg cagaatttct actgggaaat caagttattg ataataattt gcaggtgtgt 1560gaacctcagg caatatatca aacgaattct atatcaaaac cggtgctcac cacgaacaca 1620gaacgtaaag ttgcagaact cacgttcaaa gtagtctcag aagaagctaa acggttaacc 1680agtagccacc aactcagcac cccagaggtg aaagcaagcg taactcggcg agtacaacaa 1740gccttacgtg aatgggaaat ttcccaatct caaatttcat ccacttcgga acagagcgat 1800ctgacagaaa taattgaaaa gcaagttgaa cagtcgaatt ccctatcaat ggaagatacg 1860gaagttcagg agttagtcgg aacgattacc gaaaaactga tggaatatac tatcgatatt 1920cctcgaatcg tggttttgcc agaacgcgaa gtcaattacg gatttaatga ttttaacctt 1980tcccagttag atcgtattgc gctaaaacca ggtagcaaag aactcttact cacgcatctg 2040gagaataacg aacaacgtac aatcagttgg caggaaggcg gagaagagga agaacgactt 2100gaaaattacc tcattcgcta tctgctcgac cacgatgaaa ttgattacga tgaacatgcc 2160gacatgctct ataaactggc cggacaaatg gtggggcatt tatgtagtta tcagtcccaa 2220gaagatgctg aatccgttct gaaaaatgca ggtcggcagt tggcagaatt tatatgggtg 2280caaatcaaac aaaatatgtg gacaacgcca acgggctata ctggacgtat aatacagggt 2340tttgatgtaa tacatccagc cacattcaat tttgctggta atgaaagacc gagagatttc 2400cgtgttgtga ttccagcagg agaaaaaaat aaagttcgcc agatgatttt cactggtttt 2460actaagtgct gttatcctta tcagaaattt gactctgtag atggggagct ccgtctcgca 2520caaatacttg agaatgatcc ttcagtgatt cgctggatga aacctcggcc aggtcaattc 2580cgcattgagt atgctaatgg taaaaattat gaacctgatt ttgttgtcga aacggataat 2640ggctattgtt taatcgaacc caaaaaagcg acagaaatcg acacgcctga agttaaagcc 2700aaagcacaag cggctatccg gtggtgtgag tttgcaaatc aaaatgcaga taaacttaaa 2760gggaaaactt ggcaatatgc tcttattcct cataatgaga ttgaattaag tcgttcaatc 2820tcaggattac taactgattt taagatgtca tttcaatag 2859124952PRTProvidencia stuartii 164 124Met Ser Arg Ser Asn Ala Ile Lys Ile Ala Asn Lys Ile Ser Ala Arg 1 5 10 15 Leu Ser Leu Arg Asp Pro Gln Asp Glu Ser Leu Arg Ile Leu Cys Asn 20 25 30 Val Leu Glu Gln Phe Ser Leu Ser Lys Asp Pro Asp Leu Asn Arg Trp 35 40 45 Ile Glu Leu Leu Ser Gln Gln Tyr Pro Thr Val Lys Gly Phe Glu Arg 50 55 60 Ala Phe Pro Ser Leu Cys Phe Ala Leu Ala Thr Gly Val Gly Lys Thr 65 70 75 80 Arg Leu Met Gly Ala Met Ile Thr Trp Leu Tyr Leu Thr Gly Arg Ser 85 90 95 Arg His Phe Phe Ile Leu Ser Pro Asn Leu Thr Ile Tyr Glu Lys Leu 100 105 110 Lys Met Asp Phe Leu Pro Gly Ser Pro Lys Tyr Val Phe Gln Gly Ile 115 120 125 Pro Glu Leu Ala Gln Thr Pro Pro Val Leu Ile Thr Gly Asp Asp Tyr 130 135 140 Gln Glu Gly Arg Gly Val Arg Leu Asp Tyr Ala Ile Ala Glu Ser Lys 145 150 155 160 Thr Gly Asp Leu Phe Asp Asn Glu Thr Ala Pro His Ile Asn Ile Phe 165 170 175 Asn Ile Ser Lys Ile Asn Ala Leu Glu Asn Ala Lys Gly Ala Ala Lys 180 185 190 Ser Lys Val Ala Lys Ile Arg Arg Ile Gln Glu Tyr Ile Gly Glu Ser 195 200 205 Tyr Phe Ser Tyr Leu Ala Asn Leu Pro Asp Leu Val Ile Leu Met Asp 210 215 220 Glu Ala His Arg Tyr Tyr Ala Ser Ala Gly Ala Gln Ala Leu Asn Asp 225 230 235 240 Leu Asn Pro Val Leu Gly Ile Glu Leu Thr Ala Thr Pro Lys Thr Val 245 250 255 Gly Ala Asn Pro Arg Asp Phe Lys Asn Ile Ile Tyr His Tyr Pro Leu 260 265 270 Ser Arg Ala Leu Lys Asp Gly Tyr Val Lys Ile Pro Ala Val Ala Thr 275 280 285 Arg Lys Glu Phe Arg Ala Ala Asn Tyr Ser Glu Glu Gln Leu Glu Lys 290 295 300 Ile Lys Leu Glu Asp Gly Ile His His His Glu Tyr Val Lys Thr Glu 305 310 315 320 Leu Thr Ser Phe Ala Asn Asn Thr Gly Asn Lys Leu Ile Lys Pro Phe 325 330 335 Met Leu Val Val Ala Gln Asp Thr Asp His Ala Asp Ser Leu Lys Val 340 345 350 Arg Ile Glu His Asp Asp Phe Phe Asn Gly Ala Tyr Lys Gly Lys Val 355 360 365 Ile Thr Val His Ser Asn Gln Thr Gly Glu Glu Ser Glu Glu Thr Met 370 375 380 Gln Arg Leu Leu Ala Val Glu Tyr Asp Lys Asp Thr Glu Ile Val Ile 385 390 395 400 His Val Asn Lys Leu Lys Glu Gly Trp Asp Val Thr Asn Leu Tyr Thr 405 410 415 Ile Val Pro Leu Arg Ala Ser Ala Ser Glu Ile Leu Thr Glu Gln Thr 420 425 430 Ile Gly Arg Gly Leu Arg Leu Pro Tyr Gly Lys Arg Thr Gly Val Glu 435 440 445 Ala Val Asp Arg Leu Thr Ile Ile Ala His Asp Arg Phe Gln Asp Ile 450 455 460 Ile Asp Arg Ala Asn Asn Asp Asp Ser Ile Ile Lys Lys Val Leu Tyr 465 470 475 480 Ile Gly Leu Asp Asp Asp Glu Asn Gly Ile Pro Glu Val Lys Pro Gln 485 490 495 Gln Ile Val Val Pro Ser Met Ala Glu Phe Leu Leu Gly Asn Gln Val 500 505 510 Ile Asp Asn Asn Leu Gln Val Cys Glu Pro Gln Ala Ile Tyr Gln Thr 515 520 525 Asn Ser Ile Ser Lys Pro Val Leu Thr Thr Asn Thr Glu Arg Lys Val 530 535 540 Ala Glu Leu Thr Phe Lys Val Val Ser Glu Glu Ala Lys Arg Leu Thr 545 550 555 560 Ser Ser His Gln Leu Ser Thr Pro Glu Val Lys Ala Ser Val Thr Arg 565 570 575 Arg Val Gln Gln Ala Leu Arg Glu Trp Glu Ile Ser Gln Ser Gln Ile 580 585 590 Ser Ser Thr Ser Glu Gln Ser Asp Leu Thr Glu Ile Ile Glu Lys Gln 595 600 605 Val Glu Gln Ser Asn Ser Leu Ser Met Glu Asp Thr Glu Val Gln Glu 610 615 620 Leu Val Gly Thr Ile Thr Glu Lys Leu Met Glu Tyr Thr Ile Asp Ile 625 630 635 640 Pro Arg Ile Val Val Leu Pro Glu Arg Glu Val Asn Tyr Gly Phe Asn 645 650 655 Asp Phe Asn Leu Ser Gln Leu Asp Arg Ile Ala Leu Lys Pro Gly Ser 660 665 670 Lys Glu Leu Leu Leu Thr His Leu Glu Asn Asn Glu Gln Arg Thr Ile 675 680 685 Ser Trp Gln Glu Gly Gly Glu Glu Glu Glu Arg Leu

Glu Asn Tyr Leu 690 695 700 Ile Arg Tyr Leu Leu Asp His Asp Glu Ile Asp Tyr Asp Glu His Ala 705 710 715 720 Asp Met Leu Tyr Lys Leu Ala Gly Gln Met Val Gly His Leu Cys Ser 725 730 735 Tyr Gln Ser Gln Glu Asp Ala Glu Ser Val Leu Lys Asn Ala Gly Arg 740 745 750 Gln Leu Ala Glu Phe Ile Trp Val Gln Ile Lys Gln Asn Met Trp Thr 755 760 765 Thr Pro Thr Gly Tyr Thr Gly Arg Ile Ile Gln Gly Phe Asp Val Ile 770 775 780 His Pro Ala Thr Phe Asn Phe Ala Gly Asn Glu Arg Pro Arg Asp Phe 785 790 795 800 Arg Val Val Ile Pro Ala Gly Glu Lys Asn Lys Val Arg Gln Met Ile 805 810 815 Phe Thr Gly Phe Thr Lys Cys Cys Tyr Pro Tyr Gln Lys Phe Asp Ser 820 825 830 Val Asp Gly Glu Leu Arg Leu Ala Gln Ile Leu Glu Asn Asp Pro Ser 835 840 845 Val Ile Arg Trp Met Lys Pro Arg Pro Gly Gln Phe Arg Ile Glu Tyr 850 855 860 Ala Asn Gly Lys Asn Tyr Glu Pro Asp Phe Val Val Glu Thr Asp Asn 865 870 875 880 Gly Tyr Cys Leu Ile Glu Pro Lys Lys Ala Thr Glu Ile Asp Thr Pro 885 890 895 Glu Val Lys Ala Lys Ala Gln Ala Ala Ile Arg Trp Cys Glu Phe Ala 900 905 910 Asn Gln Asn Ala Asp Lys Leu Lys Gly Lys Thr Trp Gln Tyr Ala Leu 915 920 925 Ile Pro His Asn Glu Ile Glu Leu Ser Arg Ser Ile Ser Gly Leu Leu 930 935 940 Thr Asp Phe Lys Met Ser Phe Gln 945 950 1251008DNAPseudomonas stutzeri 1660 125atgccttact cctttgatca ttcggaagtt tgccataact gtcctttcgg aagttgcttt 60gaggacagaa gggataatcc ggtaaggaat cgagatacaa aatttcggtt tcgccaaact 120gccgcaatga gctgtacatt cgcagacttc atccctggga cggatagtga ccccatcccc 180caaagaagct tcgaagagta tctaaaaaag ttcacctcga atgccatgct ggcaggtgaa 240actctgtttg gtggtgagtt caacgttaag ggtgcggcca tcgccaaagt cgaaggtgat 300gtctttgaac tccttgaggc tgcggcgctg tggaatgcta cagcggcttg gaacaggctt 360atggactccg gttcgtgggg cgcatctgta ttcacttgtc ctcaatcggc tgtgcctacg 420ccgacccgta aaattgccgt agtaaccctt ccaagagggt atgatgcaac aaagctattt 480cgggacgaaa ttcgcagcag catccgtgct cacgaggagg ctcttcatct aagagggttg 540tctcttgggt tgtcaagtcc tgacattgtt ggtgttcggc ttccttgccc gcttccggaa 600gagcttggtt gcttcatgga gcctatcgaa aaccttggtg aagaaaacag ggtaaagctt 660gaagaggctt acaagctgct tgaaggcaag attgaggcaa caggtttcct ctttgctatt 720gccgtaaaga gaaccattcg gagtgatagg ctttaccagc cgcttttcga agccaatgtg 780ttgaagtatc tgattgaggt ggtgctcaag ggggctgcgt tcaggttcta cgctcacttc 840aattcatttg aaggtgcgga tgtggaaggt cactacaagg cggcatcgct aatctcgcta 900gccaggggtg gcactcctac aaaagcgatt gacgttctcc accttgcgga gtctcccctt 960gcatcggcac aggctgtgct taatgacttt cccctgttcc atctttaa 1008126335PRTPseudomonas stutzeri 1660 126Met Pro Tyr Ser Phe Asp His Ser Glu Val Cys His Asn Cys Pro Phe 1 5 10 15 Gly Ser Cys Phe Glu Asp Arg Arg Asp Asn Pro Val Arg Asn Arg Asp 20 25 30 Thr Lys Phe Arg Phe Arg Gln Thr Ala Ala Met Ser Cys Thr Phe Ala 35 40 45 Asp Phe Ile Pro Gly Thr Asp Ser Asp Pro Ile Pro Gln Arg Ser Phe 50 55 60 Glu Glu Tyr Leu Lys Lys Phe Thr Ser Asn Ala Met Leu Ala Gly Glu 65 70 75 80 Thr Leu Phe Gly Gly Glu Phe Asn Val Lys Gly Ala Ala Ile Ala Lys 85 90 95 Val Glu Gly Asp Val Phe Glu Leu Leu Glu Ala Ala Ala Leu Trp Asn 100 105 110 Ala Thr Ala Ala Trp Asn Arg Leu Met Asp Ser Gly Ser Trp Gly Ala 115 120 125 Ser Val Phe Thr Cys Pro Gln Ser Ala Val Pro Thr Pro Thr Arg Lys 130 135 140 Ile Ala Val Val Thr Leu Pro Arg Gly Tyr Asp Ala Thr Lys Leu Phe 145 150 155 160 Arg Asp Glu Ile Arg Ser Ser Ile Arg Ala His Glu Glu Ala Leu His 165 170 175 Leu Arg Gly Leu Ser Leu Gly Leu Ser Ser Pro Asp Ile Val Gly Val 180 185 190 Arg Leu Pro Cys Pro Leu Pro Glu Glu Leu Gly Cys Phe Met Glu Pro 195 200 205 Ile Glu Asn Leu Gly Glu Glu Asn Arg Val Lys Leu Glu Glu Ala Tyr 210 215 220 Lys Leu Leu Glu Gly Lys Ile Glu Ala Thr Gly Phe Leu Phe Ala Ile 225 230 235 240 Ala Val Lys Arg Thr Ile Arg Ser Asp Arg Leu Tyr Gln Pro Leu Phe 245 250 255 Glu Ala Asn Val Leu Lys Tyr Leu Ile Glu Val Val Leu Lys Gly Ala 260 265 270 Ala Phe Arg Phe Tyr Ala His Phe Asn Ser Phe Glu Gly Ala Asp Val 275 280 285 Glu Gly His Tyr Lys Ala Ala Ser Leu Ile Ser Leu Ala Arg Gly Gly 290 295 300 Thr Pro Thr Lys Ala Ile Asp Val Leu His Leu Ala Glu Ser Pro Leu 305 310 315 320 Ala Ser Ala Gln Ala Val Leu Asn Asp Phe Pro Leu Phe His Leu 325 330 335 127603DNAStreptomyces achromogenes 127atggcgccgg tggtgagccc cgacgatggc acgcagtacc acaaggactt cactctcagc 60atcacgaagg cgctcggtga ccagctggca gcggctctgg acgggctaga cagggccccc 120ctgacggacc ggagcatcgc ggccctcaag gaaaagcccg gcgtctacca gctctacttg 180aacggcagct tcgtctacgt cggcaaggct gataggtcgt tgcccgcgcg gctccgcaac 240cataagcgca agatctcggg gcgtcggagg atttcgctcg acgagatggc cttctcctgt 300ctctacgtgg ccgaagactt ctcggcactc gcccccgaac agctcctgat cagccaccac 360aagggcatgg gagacattcc ctggaacaac aacgggttcg gcaataagga ccccgggcgc 420cagcgggaca gcaccgtact aaagcggaat cactttgacg tgctattccc catcgacctc 480gaccggtcga gggcctacga gccggggaaa cgacactgca ggagctcctg gaagcggtta 540aggtcggttt gccctacaac ttccgctatg ggaagcatga cggcttcaag agtcgatatg 600tga 603128200PRTStreptomyces achromogenes 128Met Ala Pro Val Val Ser Pro Asp Asp Gly Thr Gln Tyr His Lys Asp 1 5 10 15 Phe Thr Leu Ser Ile Thr Lys Ala Leu Gly Asp Gln Leu Ala Ala Ala 20 25 30 Leu Asp Gly Leu Asp Arg Ala Pro Leu Thr Asp Arg Ser Ile Ala Ala 35 40 45 Leu Lys Glu Lys Pro Gly Val Tyr Gln Leu Tyr Leu Asn Gly Ser Phe 50 55 60 Val Tyr Val Gly Lys Ala Asp Arg Ser Leu Pro Ala Arg Leu Arg Asn 65 70 75 80 His Lys Arg Lys Ile Ser Gly Arg Arg Arg Ile Ser Leu Asp Glu Met 85 90 95 Ala Phe Ser Cys Leu Tyr Val Ala Glu Asp Phe Ser Ala Leu Ala Pro 100 105 110 Glu Gln Leu Leu Ile Ser His His Lys Gly Met Gly Asp Ile Pro Trp 115 120 125 Asn Asn Asn Gly Phe Gly Asn Lys Asp Pro Gly Arg Gln Arg Asp Ser 130 135 140 Thr Val Leu Lys Arg Asn His Phe Asp Val Leu Phe Pro Ile Asp Leu 145 150 155 160 Asp Arg Ser Arg Ala Tyr Glu Pro Gly Lys Arg His Cys Arg Ser Ser 165 170 175 Trp Lys Arg Leu Arg Ser Val Cys Pro Thr Thr Ser Ala Met Gly Ser 180 185 190 Met Thr Ala Ser Arg Val Asp Met 195 200 129564DNASerratia fonticola 129atgaataata cattggatga ggcctttgct ttctatgcca gccatatcta tgacgaagaa 60aaaattaatc tgttgaggtc acataacctc aaagttgcgg ggcatgttcc ctctgtctta 120tgggagctgt ttggttcaat tcttacagga cgtcgtggta atggcattac tggggcagac 180cttcaaggct gggaggttaa gtcgtccaca ttgaggagct ctttcgagta tcagtatcac 240ttgaatacag gcgaagctaa gcttttggaa gattgcgaag ttaatcatct cttttgctcc 300tattcaactg attatcgtga tcttatcgtc aaagcgattc cgggtgagga acttaaagag 360accttttttg aagcttggtt gccagaatat agagcgaatt atgaccgtac tgtaggtagc 420acttctaggc gccaacgttt taggaaggca ataccatatg gttttgttca agtacatggt 480cgtacaatcc ttgaagttaa agctggtgaa atgtacagta gaaatgatag tcttttagaa 540gagttcaata gattggtagg ctag 564130187PRTSerratia fonticola 130Met Asn Asn Thr Leu Asp Glu Ala Phe Ala Phe Tyr Ala Ser His Ile 1 5 10 15 Tyr Asp Glu Glu Lys Ile Asn Leu Leu Arg Ser His Asn Leu Lys Val 20 25 30 Ala Gly His Val Pro Ser Val Leu Trp Glu Leu Phe Gly Ser Ile Leu 35 40 45 Thr Gly Arg Arg Gly Asn Gly Ile Thr Gly Ala Asp Leu Gln Gly Trp 50 55 60 Glu Val Lys Ser Ser Thr Leu Arg Ser Ser Phe Glu Tyr Gln Tyr His 65 70 75 80 Leu Asn Thr Gly Glu Ala Lys Leu Leu Glu Asp Cys Glu Val Asn His 85 90 95 Leu Phe Cys Ser Tyr Ser Thr Asp Tyr Arg Asp Leu Ile Val Lys Ala 100 105 110 Ile Pro Gly Glu Glu Leu Lys Glu Thr Phe Phe Glu Ala Trp Leu Pro 115 120 125 Glu Tyr Arg Ala Asn Tyr Asp Arg Thr Val Gly Ser Thr Ser Arg Arg 130 135 140 Gln Arg Phe Arg Lys Ala Ile Pro Tyr Gly Phe Val Gln Val His Gly 145 150 155 160 Arg Thr Ile Leu Glu Val Lys Ala Gly Glu Met Tyr Ser Arg Asn Asp 165 170 175 Ser Leu Leu Glu Glu Phe Asn Arg Leu Val Gly 180 185 131567DNASphaerotilus natans 131atgtcaatcg atcccaacaa gctaaacagc gccctttacg cgattcttgg aggctacaga 60ggaaaattct ccaataaggt ctataacggc gaaaacgatg agttcgacat tttaatggaa 120attttcggaa tttccccatt attgaaacgc gagagccgcc agtactgggg ccgagagctt 180ggcatgtgct ggccacgact tgttgtggaa atttgcaaac agacgcgaaa tgacttcgga 240tctgctttac aaattgatgg cggcgagcct tgtgatttga tagtaggcgg tttggcgatc 300gaaaccaagt atagaatagg gtccggcgat gcaggcacct tgaaaaagtt ccaagcttac 360ggctctctgc ttagttcaat ggggtatgag ccagtactct tgatagttcg tgaagacaac 420cttggtgcag caatcacagc gtgccacgca ggcggctgga ccgttataac agggcaacgc 480accttcgact accttcgcga ccttacagga attaacatta aggaactact cctgcagcgt 540gccggaaaat ttcctgttgt ccggtga 567132188PRTSphaerotilus natans 132Met Ser Ile Asp Pro Asn Lys Leu Asn Ser Ala Leu Tyr Ala Ile Leu 1 5 10 15 Gly Gly Tyr Arg Gly Lys Phe Ser Asn Lys Val Tyr Asn Gly Glu Asn 20 25 30 Asp Glu Phe Asp Ile Leu Met Glu Ile Phe Gly Ile Ser Pro Leu Leu 35 40 45 Lys Arg Glu Ser Arg Gln Tyr Trp Gly Arg Glu Leu Gly Met Cys Trp 50 55 60 Pro Arg Leu Val Val Glu Ile Cys Lys Gln Thr Arg Asn Asp Phe Gly 65 70 75 80 Ser Ala Leu Gln Ile Asp Gly Gly Glu Pro Cys Asp Leu Ile Val Gly 85 90 95 Gly Leu Ala Ile Glu Thr Lys Tyr Arg Ile Gly Ser Gly Asp Ala Gly 100 105 110 Thr Leu Lys Lys Phe Gln Ala Tyr Gly Ser Leu Leu Ser Ser Met Gly 115 120 125 Tyr Glu Pro Val Leu Leu Ile Val Arg Glu Asp Asn Leu Gly Ala Ala 130 135 140 Ile Thr Ala Cys His Ala Gly Gly Trp Thr Val Ile Thr Gly Gln Arg 145 150 155 160 Thr Phe Asp Tyr Leu Arg Asp Leu Thr Gly Ile Asn Ile Lys Glu Leu 165 170 175 Leu Leu Gln Arg Ala Gly Lys Phe Pro Val Val Arg 180 185 133846DNAThermococcus litoralis 133atggaaacta gcgtttgcca tactttgaag agccctgtta ttaagaagtt ctgtgagtct 60ataactgaat tggccagaac ctctagggga tacttcgagc ctattcagga cgattttctg 120aaagcatact atcaaatcgt tgaaaaagct cgtattaacg gcagacttcc agagggagaa 180taccgacaga aaggaaacgc atttagagat tttatcagcg aattgattta cataagatct 240gggggaatct accgcctaac agatagaaga attcctggct attctgagag aactcatgac 300gttgatctcg cttatgtgag ggacgctact gttttggtgg ctggcgaagt caaaatgaca 360ggtagcccaa ggcataagaa gggaacaacg gttcagaagg aaagaaagac gcagagcgat 420ctagataaaa gattaaaaga agtcaagttc accgcagtgg atttaaaact tcgctacact 480cccgaagagg ccataataaa tgccttaaac tccaagaata ctttttctga agtttctaat 540aacagttggt ggatgcgatg gattcatacc tccattcccg gcttttactc gttctgggca 600tccaggcttg cctcgggccg tcttgacaag aaaacaggaa ggagagtaga ctttgataat 660cccgatcttc ttctcgaaaa attcaggaat ctactaaaat acaacaacgc agtaggtctt 720ttcatgttcc gggaggagaa tggcagatac gttcccgttg aaactgagag aatcaaaagg 780gaaagaattt caatagacga cgcggtgaag gatcttataa agttcctaga tactcacttg 840gattag 846134281PRTThermococcus litoralis 134Met Glu Thr Ser Val Cys His Thr Leu Lys Ser Pro Val Ile Lys Lys 1 5 10 15 Phe Cys Glu Ser Ile Thr Glu Leu Ala Arg Thr Ser Arg Gly Tyr Phe 20 25 30 Glu Pro Ile Gln Asp Asp Phe Leu Lys Ala Tyr Tyr Gln Ile Val Glu 35 40 45 Lys Ala Arg Ile Asn Gly Arg Leu Pro Glu Gly Glu Tyr Arg Gln Lys 50 55 60 Gly Asn Ala Phe Arg Asp Phe Ile Ser Glu Leu Ile Tyr Ile Arg Ser 65 70 75 80 Gly Gly Ile Tyr Arg Leu Thr Asp Arg Arg Ile Pro Gly Tyr Ser Glu 85 90 95 Arg Thr His Asp Val Asp Leu Ala Tyr Val Arg Asp Ala Thr Val Leu 100 105 110 Val Ala Gly Glu Val Lys Met Thr Gly Ser Pro Arg His Lys Lys Gly 115 120 125 Thr Thr Val Gln Lys Glu Arg Lys Thr Gln Ser Asp Leu Asp Lys Arg 130 135 140 Leu Lys Glu Val Lys Phe Thr Ala Val Asp Leu Lys Leu Arg Tyr Thr 145 150 155 160 Pro Glu Glu Ala Ile Ile Asn Ala Leu Asn Ser Lys Asn Thr Phe Ser 165 170 175 Glu Val Ser Asn Asn Ser Trp Trp Met Arg Trp Ile His Thr Ser Ile 180 185 190 Pro Gly Phe Tyr Ser Phe Trp Ala Ser Arg Leu Ala Ser Gly Arg Leu 195 200 205 Asp Lys Lys Thr Gly Arg Arg Val Asp Phe Asp Asn Pro Asp Leu Leu 210 215 220 Leu Glu Lys Phe Arg Asn Leu Leu Lys Tyr Asn Asn Ala Val Gly Leu 225 230 235 240 Phe Met Phe Arg Glu Glu Asn Gly Arg Tyr Val Pro Val Glu Thr Glu 245 250 255 Arg Ile Lys Arg Glu Arg Ile Ser Ile Asp Asp Ala Val Lys Asp Leu 260 265 270 Ile Lys Phe Leu Asp Thr His Leu Asp 275 280 1351137DNAThermus species 93170 135atgaaaagat tagcaggctt aataagctta gcagacttaa tacaaggtga tactgagttt 60aagataagct gggaaaaccg agggaaaaag gcgctcactc ttctggccga gaaggcaggc 120atcagatgcg acgagcagct agatgatctt ctgtcgcaag ccctggatct tgcaaggagc 180acgcttacct ccggcaaaaa tcctgatgct gacatcgctc acttctggga ggaggtcgaa 240aaaaacgcca ccctcttaac gaaaaacgac tacctccgag cggctgtagt agctctttcg 300tttgcccacc gctttgcccg aacagactac ggatcgtcaa ggcaacgcgg cttcgggcaa 360ctctggggag atgcgattca aggcttcctt ggtgaaattg ccttccagaa gtttatgagg 420tcagccacgt ctgggaggac catccctatt ttagacgcca gcgaagaaga tcttggagtc 480gccctaagcg ctgacatagt tgaagtcatc acagagggga aatcaataaa gccctcaaaa 540agaatcagca tcaagactac gaagctccat gggcgctggt tagatgtacc ctacgctcaa 600aataagcaca gcgacattta cgttctggtt aaagtcggga ctgacgccga tgcgcttttc 660aactttctgg caagcgtagg ggcgcttgag aaagtcttaa ccgcctatca agagggcggt 720cttgctgaag gcgagcttcc ttttctcaac gaaggcgaag cgctcaaaag agctaaggaa 780gaggtagaaa aaatgaagga aaaaaacatg ctttttttag cctttatagc tggctggaag 840gagaaggatc ggctcagcca aaccttcgaa gctcacgagc acaacgccca aagagcccgc 900acaaaaatca ctgtctacag cggagttggt acaatttcat ctggtagcgt gcgaacaaag 960caaatcacct ttcgcggtcc cctccctaaa aacaatctgc tggttgagtt ttatccaata 1020ggaaaattct ccaaaagcca gcatgcactg tgcagcacag atctgcttgt gaaggatctc 1080aataagatag cagaacttct ctctgctcct gaagaggggg atgaatgcgc acagtaa 1137136378PRTThermus species 93170 136Met Lys Arg Leu Ala Gly Leu Ile Ser Leu Ala Asp Leu Ile Gln Gly 1 5 10 15 Asp Thr Glu Phe Lys Ile Ser Trp Glu Asn Arg Gly Lys Lys Ala Leu 20 25 30 Thr Leu Leu Ala Glu Lys Ala Gly Ile Arg Cys Asp Glu Gln Leu Asp 35

40 45 Asp Leu Leu Ser Gln Ala Leu Asp Leu Ala Arg Ser Thr Leu Thr Ser 50 55 60 Gly Lys Asn Pro Asp Ala Asp Ile Ala His Phe Trp Glu Glu Val Glu 65 70 75 80 Lys Asn Ala Thr Leu Leu Thr Lys Asn Asp Tyr Leu Arg Ala Ala Val 85 90 95 Val Ala Leu Ser Phe Ala His Arg Phe Ala Arg Thr Asp Tyr Gly Ser 100 105 110 Ser Arg Gln Arg Gly Phe Gly Gln Leu Trp Gly Asp Ala Ile Gln Gly 115 120 125 Phe Leu Gly Glu Ile Ala Phe Gln Lys Phe Met Arg Ser Ala Thr Ser 130 135 140 Gly Arg Thr Ile Pro Ile Leu Asp Ala Ser Glu Glu Asp Leu Gly Val 145 150 155 160 Ala Leu Ser Ala Asp Ile Val Glu Val Ile Thr Glu Gly Lys Ser Ile 165 170 175 Lys Pro Ser Lys Arg Ile Ser Ile Lys Thr Thr Lys Leu His Gly Arg 180 185 190 Trp Leu Asp Val Pro Tyr Ala Gln Asn Lys His Ser Asp Ile Tyr Val 195 200 205 Leu Val Lys Val Gly Thr Asp Ala Asp Ala Leu Phe Asn Phe Leu Ala 210 215 220 Ser Val Gly Ala Leu Glu Lys Val Leu Thr Ala Tyr Gln Glu Gly Gly 225 230 235 240 Leu Ala Glu Gly Glu Leu Pro Phe Leu Asn Glu Gly Glu Ala Leu Lys 245 250 255 Arg Ala Lys Glu Glu Val Glu Lys Met Lys Glu Lys Asn Met Leu Phe 260 265 270 Leu Ala Phe Ile Ala Gly Trp Lys Glu Lys Asp Arg Leu Ser Gln Thr 275 280 285 Phe Glu Ala His Glu His Asn Ala Gln Arg Ala Arg Thr Lys Ile Thr 290 295 300 Val Tyr Ser Gly Val Gly Thr Ile Ser Ser Gly Ser Val Arg Thr Lys 305 310 315 320 Gln Ile Thr Phe Arg Gly Pro Leu Pro Lys Asn Asn Leu Leu Val Glu 325 330 335 Phe Tyr Pro Ile Gly Lys Phe Ser Lys Ser Gln His Ala Leu Cys Ser 340 345 350 Thr Asp Leu Leu Val Lys Asp Leu Asn Lys Ile Ala Glu Leu Leu Ser 355 360 365 Ala Pro Glu Glu Gly Asp Glu Cys Ala Gln 370 375 137855DNAThermus species 137atgaacgaaa tgtacgagat tgccaaagga gtggcctcat ttgaaggtgc tcctacctta 60ccaggacgta cgacaggtga ggctagaggg ggccgagagt ttgaagctgt tgttgcggaa 120ggtcttctga aatatggccg attgctggtc accgccgttc cctcattaag attacgcccg 180gttgctgcag aaggaacttc aagacaaaac catctggctg acgctcttgc agtcgtaaac 240gaagaaaata aaagagtctt ggtgttcaga ttacctgcat tcaggcacaa tcctctcttt 300gctgagatta cttcaggcgc gctacagaac gatttcgttc gagttccgga ctcatttttg 360aaaagggagt tcgttgtgga ggagtggtat acccccaagt taggggaact agcagaaaga 420ggatggattc ctgaagagga tgaaccttat cctttttccg ggactaacta tccagaactg 480tataggcgta agcgcaccca gttcgacggt gtgattatct tcttggaaag tggcacgcta 540agggaaaaag ccctgctaga aataaaatct ctgaagtctt ctgagggggc cagggtcgat 600ggtaacgccc acgaacggtt tgcgtaccag aatctagact atctcgagat aggggcccta 660tatcctcgca caacgctctt gctacttaca aacgatgcca ttctcaagta cagaaacaaa 720taccacacgg gaatcggtgt acatgcatta cggctaagct atgcgttttg ctggtacaag 780tttgagatgg ttagctccgt tcgacagtac cttcgcctct tttctttgtg gaaggaatgg 840ctggagggca aatga 855138284PRTThermus species 138Met Asn Glu Met Tyr Glu Ile Ala Lys Gly Val Ala Ser Phe Glu Gly 1 5 10 15 Ala Pro Thr Leu Pro Gly Arg Thr Thr Gly Glu Ala Arg Gly Gly Arg 20 25 30 Glu Phe Glu Ala Val Val Ala Glu Gly Leu Leu Lys Tyr Gly Arg Leu 35 40 45 Leu Val Thr Ala Val Pro Ser Leu Arg Leu Arg Pro Val Ala Ala Glu 50 55 60 Gly Thr Ser Arg Gln Asn His Leu Ala Asp Ala Leu Ala Val Val Asn 65 70 75 80 Glu Glu Asn Lys Arg Val Leu Val Phe Arg Leu Pro Ala Phe Arg His 85 90 95 Asn Pro Leu Phe Ala Glu Ile Thr Ser Gly Ala Leu Gln Asn Asp Phe 100 105 110 Val Arg Val Pro Asp Ser Phe Leu Lys Arg Glu Phe Val Val Glu Glu 115 120 125 Trp Tyr Thr Pro Lys Leu Gly Glu Leu Ala Glu Arg Gly Trp Ile Pro 130 135 140 Glu Glu Asp Glu Pro Tyr Pro Phe Ser Gly Thr Asn Tyr Pro Glu Leu 145 150 155 160 Tyr Arg Arg Lys Arg Thr Gln Phe Asp Gly Val Ile Ile Phe Leu Glu 165 170 175 Ser Gly Thr Leu Arg Glu Lys Ala Leu Leu Glu Ile Lys Ser Leu Lys 180 185 190 Ser Ser Glu Gly Ala Arg Val Asp Gly Asn Ala His Glu Arg Phe Ala 195 200 205 Tyr Gln Asn Leu Asp Tyr Leu Glu Ile Gly Ala Leu Tyr Pro Arg Thr 210 215 220 Thr Leu Leu Leu Leu Thr Asn Asp Ala Ile Leu Lys Tyr Arg Asn Lys 225 230 235 240 Tyr His Thr Gly Ile Gly Val His Ala Leu Arg Leu Ser Tyr Ala Phe 245 250 255 Cys Trp Tyr Lys Phe Glu Met Val Ser Ser Val Arg Gln Tyr Leu Arg 260 265 270 Leu Phe Ser Leu Trp Lys Glu Trp Leu Glu Gly Lys 275 280 139960DNAThermus thermophilus strain 111 139atggcgaata ctcttgagga ccatattact caagtattgg agagctttaa gggcgaagaa 60ataaacagag ttatagcgat ttataagccg ccggatctcg agttagcaat tttttactca 120aaaatcatat ccaagttgtc cccgataatc ggtaacgtgc ttgaaagatc cgttgcaaag 180gaattggggg ttcgattgaa ggccccatat aagaggcaag atccggaatt tccagatgtt 240gttgtggaat tgggaaaaga taaaaggata ggctttgaaa taaaagcgtg gtatgctctt 300tcaacagaag cggctgcccg gttcagaaca agccaaaagg agctctcaag cggggcttac 360gaggaggttt atctagtcgt aatagcttgg acaatgagca agctgtttta tggaaaacct 420aaaataatca atcttttctt tgaaaaagcc attgagattg cccggacacg tgaccaaaag 480taccacaatc ctccatggaa tatagtttta gagcctgtag acacatcagc gagaacaata 540aacctacaac agaaagtagt catcggtaaa aaactacaag aagaaaatct tccagaaggt 600gtacaagccg aagaagagct taaaaagctt gctcaggaca aaaaaataaa agactataag 660gtctattcaa cgcaagaaga ttatgttgat ttcatacgaa atctggaaag agttttgcct 720tatcgcgaag actccaattt tggcaagata gatcgcattc ctcatgaaag gctttcatct 780ttcctcaaaa acaccaagaa attaaagcta ttagggctca ctttgaagga ctggatcaag 840gtcatggagt acatatcaaa tcaggaggaa aaatctgctc aaaaatctaa aaaaaagaag 900gagttagagg atctagtgga aaaagcacta aaaaagctcg gttaccctaa cacatactga 960140319PRTThermus thermophilus strain 111 140Met Ala Asn Thr Leu Glu Asp His Ile Thr Gln Val Leu Glu Ser Phe 1 5 10 15 Lys Gly Glu Glu Ile Asn Arg Val Ile Ala Ile Tyr Lys Pro Pro Asp 20 25 30 Leu Glu Leu Ala Ile Phe Tyr Ser Lys Ile Ile Ser Lys Leu Ser Pro 35 40 45 Ile Ile Gly Asn Val Leu Glu Arg Ser Val Ala Lys Glu Leu Gly Val 50 55 60 Arg Leu Lys Ala Pro Tyr Lys Arg Gln Asp Pro Glu Phe Pro Asp Val 65 70 75 80 Val Val Glu Leu Gly Lys Asp Lys Arg Ile Gly Phe Glu Ile Lys Ala 85 90 95 Trp Tyr Ala Leu Ser Thr Glu Ala Ala Ala Arg Phe Arg Thr Ser Gln 100 105 110 Lys Glu Leu Ser Ser Gly Ala Tyr Glu Glu Val Tyr Leu Val Val Ile 115 120 125 Ala Trp Thr Met Ser Lys Leu Phe Tyr Gly Lys Pro Lys Ile Ile Asn 130 135 140 Leu Phe Phe Glu Lys Ala Ile Glu Ile Ala Arg Thr Arg Asp Gln Lys 145 150 155 160 Tyr His Asn Pro Pro Trp Asn Ile Val Leu Glu Pro Val Asp Thr Ser 165 170 175 Ala Arg Thr Ile Asn Leu Gln Gln Lys Val Val Ile Gly Lys Lys Leu 180 185 190 Gln Glu Glu Asn Leu Pro Glu Gly Val Gln Ala Glu Glu Glu Leu Lys 195 200 205 Lys Leu Ala Gln Asp Lys Lys Ile Lys Asp Tyr Lys Val Tyr Ser Thr 210 215 220 Gln Glu Asp Tyr Val Asp Phe Ile Arg Asn Leu Glu Arg Val Leu Pro 225 230 235 240 Tyr Arg Glu Asp Ser Asn Phe Gly Lys Ile Asp Arg Ile Pro His Glu 245 250 255 Arg Leu Ser Ser Phe Leu Lys Asn Thr Lys Lys Leu Lys Leu Leu Gly 260 265 270 Leu Thr Leu Lys Asp Trp Ile Lys Val Met Glu Tyr Ile Ser Asn Gln 275 280 285 Glu Glu Lys Ser Ala Gln Lys Ser Lys Lys Lys Lys Glu Leu Glu Asp 290 295 300 Leu Val Glu Lys Ala Leu Lys Lys Leu Gly Tyr Pro Asn Thr Tyr 305 310 315 141792DNAXanthomonas campestris 141atgccagtaa cacctccaca agacttgatc gattttatcg atgatattct ctcggatttg 60cttacaaaca atccgcttgc cacttcctca gaggcgtatg tgcaaaacca catcgaattt 120gagcttgtga ggaggaatca caatcctaag tattatttga ggatcggcat caactaccat 180ggggagaaag tccagcatat catggtcgac ccgctcacag ggaagctaat tggctggaac 240cctgcgggag atgcgctcgg caccggtgca aacgcgaaaa tgctactcgc gaattacaca 300atatttgatc gtgcgcctgg cgaacacatg atgaccgact gcaaagttgg cggcggcccc 360ctcgcggcgg gtcagtacgt ccgcgccgag ttcaaggtga ggggatggct tggcaaaaca 420aagaatctgg atggcaagca atttcagaaa gatttggacc tcatgggcgc cgataaagcg 480gacctgcttg tttggtgcct gtcagaaacc gctcattgca agttccgcgg cgaaggacct 540gcacatcaag cgggtcgacg aacaggatgc caggatttcg cacccattct cctgcccact 600aatcaaatcg ggatcgctcc tgtaacccgg caggtcccat atcgccggat cgagaccgca 660aatttgccag ccgcgcaagc tttgtggatc aacacgcaaa attgggtcgt tcgcagtcgg 720aaggtgacgg ctgcgccggg ctcactgatg cccggcgcag agcactacgt cacgatgtgc 780tggcgcgtgt ag 792142263PRTXanthomonas campestris 142Met Pro Val Thr Pro Pro Gln Asp Leu Ile Asp Phe Ile Asp Asp Ile 1 5 10 15 Leu Ser Asp Leu Leu Thr Asn Asn Pro Leu Ala Thr Ser Ser Glu Ala 20 25 30 Tyr Val Gln Asn His Ile Glu Phe Glu Leu Val Arg Arg Asn His Asn 35 40 45 Pro Lys Tyr Tyr Leu Arg Ile Gly Ile Asn Tyr His Gly Glu Lys Val 50 55 60 Gln His Ile Met Val Asp Pro Leu Thr Gly Lys Leu Ile Gly Trp Asn 65 70 75 80 Pro Ala Gly Asp Ala Leu Gly Thr Gly Ala Asn Ala Lys Met Leu Leu 85 90 95 Ala Asn Tyr Thr Ile Phe Asp Arg Ala Pro Gly Glu His Met Met Thr 100 105 110 Asp Cys Lys Val Gly Gly Gly Pro Leu Ala Ala Gly Gln Tyr Val Arg 115 120 125 Ala Glu Phe Lys Val Arg Gly Trp Leu Gly Lys Thr Lys Asn Leu Asp 130 135 140 Gly Lys Gln Phe Gln Lys Asp Leu Asp Leu Met Gly Ala Asp Lys Ala 145 150 155 160 Asp Leu Leu Val Trp Cys Leu Ser Glu Thr Ala His Cys Lys Phe Arg 165 170 175 Gly Glu Gly Pro Ala His Gln Ala Gly Arg Arg Thr Gly Cys Gln Asp 180 185 190 Phe Ala Pro Ile Leu Leu Pro Thr Asn Gln Ile Gly Ile Ala Pro Val 195 200 205 Thr Arg Gln Val Pro Tyr Arg Arg Ile Glu Thr Ala Asn Leu Pro Ala 210 215 220 Ala Gln Ala Leu Trp Ile Asn Thr Gln Asn Trp Val Val Arg Ser Arg 225 230 235 240 Lys Val Thr Ala Ala Pro Gly Ser Leu Met Pro Gly Ala Glu His Tyr 245 250 255 Val Thr Met Cys Trp Arg Val 260 143624DNAXanthomonas holcicola 143ttgaaagtcg caaaaatcta ctcacatctg aacggcctgg aatttttaaa agttcaccat 60gaaaaggtga ttttggagct ggatcgggtc attacccgta tagacgctga ggcttgccgc 120accaaagaaa caaaagaggc aagaaaggca gggcggttcg ctgatggtct tctgtatagc 180ccggtagcac tgaatgaagc ttttaacgat gctctgtcgc agcttcactg gtatgaagat 240cgctattcct acttcgtgac cgatgacgct aggctcatta gagcgacatt agggttggac 300agagcggagc aaaagcgaat aattgaggat gcaggtcata aggcgattgc aacctacaat 360cagacggatt ttgtgaaaga cagggtggcc atagaagtgc agtttggaaa gtattcgttt 420gtagcttacg atctttttgt caaacacatg gcattttatg ttggtgacaa aattgacgtc 480ggcatagaaa ttctgccaat gaaatctttg caggagaata tgtcttcggg aattgcttac 540tacgaaagtg agctttccaa tcttgtaagg caaggccgag gtgtgcccgc tgttcctctg 600gtgctgatgg gcatagagcc ttaa 624144207PRTXanthomonas holcicola 144Leu Lys Val Ala Lys Ile Tyr Ser His Leu Asn Gly Leu Glu Phe Leu 1 5 10 15 Lys Val His His Glu Lys Val Ile Leu Glu Leu Asp Arg Val Ile Thr 20 25 30 Arg Ile Asp Ala Glu Ala Cys Arg Thr Lys Glu Thr Lys Glu Ala Arg 35 40 45 Lys Ala Gly Arg Phe Ala Asp Gly Leu Leu Tyr Ser Pro Val Ala Leu 50 55 60 Asn Glu Ala Phe Asn Asp Ala Leu Ser Gln Leu His Trp Tyr Glu Asp 65 70 75 80 Arg Tyr Ser Tyr Phe Val Thr Asp Asp Ala Arg Leu Ile Arg Ala Thr 85 90 95 Leu Gly Leu Asp Arg Ala Glu Gln Lys Arg Ile Ile Glu Asp Ala Gly 100 105 110 His Lys Ala Ile Ala Thr Tyr Asn Gln Thr Asp Phe Val Lys Asp Arg 115 120 125 Val Ala Ile Glu Val Gln Phe Gly Lys Tyr Ser Phe Val Ala Tyr Asp 130 135 140 Leu Phe Val Lys His Met Ala Phe Tyr Val Gly Asp Lys Ile Asp Val 145 150 155 160 Gly Ile Glu Ile Leu Pro Met Lys Ser Leu Gln Glu Asn Met Ser Ser 165 170 175 Gly Ile Ala Tyr Tyr Glu Ser Glu Leu Ser Asn Leu Val Arg Gln Gly 180 185 190 Arg Gly Val Pro Ala Val Pro Leu Val Leu Met Gly Ile Glu Pro 195 200 205 145855DNABacillus brevis 145tttaaccaat ttaatccgtt agtatataca cacggtggaa aacttgaacg gaagtctaag 60aaggataaaa cagcaagtaa ggtgttcgaa gaatttggtg tgatggaggc ttataattgt 120tggaaggaag cttccctatg tattcaacaa agagacaagg atagcgttct taaacttgta 180gcagctctca atacgtataa agacgcagta gaaccaattt ttgactcaag actgaatagt 240gcccaagaag ttctccaacc gtcgatttta gaagaatttt ttgaatatct gtttagcagg 300attgactcta ttgttggagt gaatattcca attcgacatc cagcgaaggg ttatttaagt 360ctcagcttta atccacataa tatagaaacg ctcatccaat cgccggagta cactgtaagg 420gcgaaggatc atgattttat tattggtggg tcagcgaaat taaccattca aggacatggc 480ggggaaggag aaacaaccaa cattgtggtt cctgctgtag cgattgaatg caagcggtac 540cttgaacgaa acatgctaga tgaatgtgct ggtactgctg agcgcttaaa aagagcaaca 600ccatattgtt tatacttcgt agttgcggag tacttaaaac tagatgatgg agcaccggaa 660ttaaccgaga ttgatgagat ttacatactt cggcaccagc ggaactcaga gcggaataag 720ccaggattta agcctaaccc catagatggt gaactgattt gggatttgta ccaagaagtt 780atgaatcatc ttgggaagat ttggtgggat ccaaactcag ctttacaacg cggtaaagtg 840tttaatcgac cataa 855146285PRTBacillus brevis 146Met Phe Asn Gln Phe Asn Pro Leu Val Tyr Thr His Gly Gly Lys Leu 1 5 10 15 Glu Arg Lys Ser Lys Lys Asp Lys Thr Ala Ser Lys Val Phe Glu Glu 20 25 30 Phe Gly Val Met Glu Ala Tyr Asn Cys Trp Lys Glu Ala Ser Leu Cys 35 40 45 Ile Gln Gln Arg Asp Lys Asp Ser Val Leu Lys Leu Val Ala Ala Leu 50 55 60 Asn Thr Tyr Lys Asp Ala Val Glu Pro Ile Phe Asp Ser Arg Leu Asn 65 70 75 80 Ser Ala Gln Glu Val Leu Gln Pro Ser Ile Leu Glu Glu Phe Phe Glu 85 90 95 Tyr Leu Phe Ser Arg Ile Asp Ser Ile Val Gly Val Asn Ile Pro Ile 100 105 110 Arg His Pro Ala Lys Gly Tyr Leu Ser Leu Ser Phe Asn Pro His Asn 115 120 125 Ile Glu Thr Leu Ile Gln Ser Pro Glu Tyr Thr Val Arg Ala Lys Asp 130 135 140 His Asp Phe Ile Ile Gly Gly Ser Ala Lys Leu Thr Ile Gln Gly His 145 150 155 160 Gly Gly Glu Gly Glu Thr Thr Asn Ile Val Val Pro Ala Val Ala Ile 165 170 175 Glu Cys Lys Arg Tyr Leu Glu Arg Asn Met Leu Asp Glu Cys Ala Gly 180 185 190 Thr Ala Glu Arg Leu Lys Arg Ala

Thr Pro Tyr Cys Leu Tyr Phe Val 195 200 205 Val Ala Glu Tyr Leu Lys Leu Asp Asp Gly Ala Pro Glu Leu Thr Glu 210 215 220 Ile Asp Glu Ile Tyr Ile Leu Arg His Gln Arg Asn Ser Glu Arg Asn 225 230 235 240 Lys Pro Gly Phe Lys Pro Asn Pro Ile Asp Gly Glu Leu Ile Trp Asp 245 250 255 Leu Tyr Gln Glu Val Met Asn His Leu Gly Lys Ile Trp Trp Asp Pro 260 265 270 Asn Ser Ala Leu Gln Arg Gly Lys Val Phe Asn Arg Pro 275 280 285 147591DNABacillus fragilis 147atgaagaaat tcaagatttc aaatgatgag gttacggagt tgtcaaatgc tcctcaatat 60caatttccaa agtatgtgac tcaggttatt aacttggtaa atagtaacgc tggcggtact 120cgccctaagg tagttggtca gatgtcagaa ctggttaaag agtttgatgg taggaccatt 180gacgagtgga ttgagtggta cacggagaga taccctgatg caattaatga tgctactgaa 240aagatctggg ccatgtatga gaccatgaag ggtgctttca atgctatcac caaagagatg 300gtcgagaatt gggtgaaaga tcttgtctat ggtaaaacct tctgtggttt gaaatttcag 360acagctatta tttcagcgat agccaatcag ttagacaagt cttggagaga ggctgatcct 420gaagaagaag ctcaaggtat tgatggcttt attggtgaca agccacttca gattaagtct 480gctacatata aattagaagc acgcctttct gaaaccatca atgcaccaat agtgtactac 540gacaagaaga aggatggcat aagtattgag tataacccaa ctgactttta a 591148196PRTBacillus fragilis 148Met Lys Lys Phe Lys Ile Ser Asn Asp Glu Val Thr Glu Leu Ser Asn 1 5 10 15 Ala Pro Gln Tyr Gln Phe Pro Lys Tyr Val Thr Gln Val Ile Asn Leu 20 25 30 Val Asn Ser Asn Ala Gly Gly Thr Arg Pro Lys Val Val Gly Gln Met 35 40 45 Ser Glu Leu Val Lys Glu Phe Asp Gly Arg Thr Ile Asp Glu Trp Ile 50 55 60 Glu Trp Tyr Thr Glu Arg Tyr Pro Asp Ala Ile Asn Asp Ala Thr Glu 65 70 75 80 Lys Ile Trp Ala Met Tyr Glu Thr Met Lys Gly Ala Phe Asn Ala Ile 85 90 95 Thr Lys Glu Met Val Glu Asn Trp Val Lys Asp Leu Val Tyr Gly Lys 100 105 110 Thr Phe Cys Gly Leu Lys Phe Gln Thr Ala Ile Ile Ser Ala Ile Ala 115 120 125 Asn Gln Leu Asp Lys Ser Trp Arg Glu Ala Asp Pro Glu Glu Glu Ala 130 135 140 Gln Gly Ile Asp Gly Phe Ile Gly Asp Lys Pro Leu Gln Ile Lys Ser 145 150 155 160 Ala Thr Tyr Lys Leu Glu Ala Arg Leu Ser Glu Thr Ile Asn Ala Pro 165 170 175 Ile Val Tyr Tyr Asp Lys Lys Lys Asp Gly Ile Ser Ile Glu Tyr Asn 180 185 190 Pro Thr Asp Phe 195 1491620DNAAcinetobacter calcoaceticus 65 149atgaaattaa atgctgagaa tttaagtatt caggaacaat tagcagaatt tgatcaatgg 60ctcacagcta gactagataa aatcaaagat tcagaaaaat tcaattcaga aattaactcc 120ctctgtaatt gtattaccgt attatctcct cttttagaaa acttcagtga tccttccacc 180tgtacaattc atagcttagt gaatgcggtt atagaagcca gcaatagaat agtctctggt 240agtagttttg gaggtgatga agctgctctc aacaactttt atgagtcttt ttttaacttg 300ctattcctaa ccagtggggc aacagataac aacctaaaga atcattttct aattaaactt 360aatgaagacg atattacacc tctcatacct aaacgtggtt caataaagaa acagatcaca 420ttcaaacttt atgaaattcc tacaactact aaatctgact ttatcgctcg taccttagca 480agttgtttta caggaactaa atatcccctc ctagtaaaga cagaaccatt tttcgatctt 540gaaacatact ttaaaatttt tttagaagaa tacattaagc ttattcttga tgatgaagaa 600gatttattac aactctgggc tatctgccac tcatttgttg aattatccac taaccctcat 660ggttccaatt tgggtaaata tttattaaat tcttgtacga tttttaaagt tagaggtagt 720gtatcagcat caggtggtca cgttactgaa tctatactta gggaaaagtt atcaaacatc 780gggttaagag ctgatattga ttacaataat aatgatgtca aaattggtga tgatgaaatt 840attgaagacg ggaaaagaaa aaagaaaact cgtgcgtatg actttataat tccttataaa 900atagataact gggaaccaaa acctaagcta tttatccaat cacaatttta cgctggggat 960tctggcagtg tatctcataa agtcgtagat caaactcaaa gttcaagagt atttacacta 1020accaaatatc cgaatgctaa atttgttgaa tatttagatg gtgctggtta ctacgcttct 1080ttaagaggtg atttacagca catgctatct ttcagcaata cagaatcttt ttttcaagta 1140aaaagtattc ttttacgttt aagacgtgaa ttccaaaaga tcgatttttt aacagctatt 1200gaaattcagc atgctgtact aatcagcaaa tctcggactc ataaagatct ccaaaatctt 1260cttataaaag ataactattc tatccaagaa atagaaagag ctattcaaac caatttagaa 1320ctaggtctta ttactaaaaa tgaatcagat gaaattgtaa tacctacaga acatatttgt 1380atcgcccgga gacttttaat tttagatatt gctgcaaact attcatgctc tattactcag 1440gcagaaaagt ctagccaaaa atatttatta gtaccgggca atggggccaa taaaggaatt 1500aaggagtcta agctagctga gttagctttt gacttatgta aagatattaa tataacaccg 1560actgaattta ttgaagacat cgaatggctc ttagatgagg gagtaattaa acgattttag 1620150539PRTAcinetobacter calcoaceticus 65 150Met Lys Leu Asn Ala Glu Asn Leu Ser Ile Gln Glu Gln Leu Ala Glu 1 5 10 15 Phe Asp Gln Trp Leu Thr Ala Arg Leu Asp Lys Ile Lys Asp Ser Glu 20 25 30 Lys Phe Asn Ser Glu Ile Asn Ser Leu Cys Asn Cys Ile Thr Val Leu 35 40 45 Ser Pro Leu Leu Glu Asn Phe Ser Asp Pro Ser Thr Cys Thr Ile His 50 55 60 Ser Leu Val Asn Ala Val Ile Glu Ala Ser Asn Arg Ile Val Ser Gly 65 70 75 80 Ser Ser Phe Gly Gly Asp Glu Ala Ala Leu Asn Asn Phe Tyr Glu Ser 85 90 95 Phe Phe Asn Leu Leu Phe Leu Thr Ser Gly Ala Thr Asp Asn Asn Leu 100 105 110 Lys Asn His Phe Leu Ile Lys Leu Asn Glu Asp Asp Ile Thr Pro Leu 115 120 125 Ile Pro Lys Arg Gly Ser Ile Lys Lys Gln Ile Thr Phe Lys Leu Tyr 130 135 140 Glu Ile Pro Thr Thr Thr Lys Ser Asp Phe Ile Ala Arg Thr Leu Ala 145 150 155 160 Ser Cys Phe Thr Gly Thr Lys Tyr Pro Leu Leu Val Lys Thr Glu Pro 165 170 175 Phe Phe Asp Leu Glu Thr Tyr Phe Lys Ile Phe Leu Glu Glu Tyr Ile 180 185 190 Lys Leu Ile Leu Asp Asp Glu Glu Asp Leu Leu Gln Leu Trp Ala Ile 195 200 205 Cys His Ser Phe Val Glu Leu Ser Thr Asn Pro His Gly Ser Asn Leu 210 215 220 Gly Lys Tyr Leu Leu Asn Ser Cys Thr Ile Phe Lys Val Arg Gly Ser 225 230 235 240 Val Ser Ala Ser Gly Gly His Val Thr Glu Ser Ile Leu Arg Glu Lys 245 250 255 Leu Ser Asn Ile Gly Leu Arg Ala Asp Ile Asp Tyr Asn Asn Asn Asp 260 265 270 Val Lys Ile Gly Asp Asp Glu Ile Ile Glu Asp Gly Lys Arg Lys Lys 275 280 285 Lys Thr Arg Ala Tyr Asp Phe Ile Ile Pro Tyr Lys Ile Asp Asn Trp 290 295 300 Glu Pro Lys Pro Lys Leu Phe Ile Gln Ser Gln Phe Tyr Ala Gly Asp 305 310 315 320 Ser Gly Ser Val Ser His Lys Val Val Asp Gln Thr Gln Ser Ser Arg 325 330 335 Val Phe Thr Leu Thr Lys Tyr Pro Asn Ala Lys Phe Val Glu Tyr Leu 340 345 350 Asp Gly Ala Gly Tyr Tyr Ala Ser Leu Arg Gly Asp Leu Gln His Met 355 360 365 Leu Ser Phe Ser Asn Thr Glu Ser Phe Phe Gln Val Lys Ser Ile Leu 370 375 380 Leu Arg Leu Arg Arg Glu Phe Gln Lys Ile Asp Phe Leu Thr Ala Ile 385 390 395 400 Glu Ile Gln His Ala Val Leu Ile Ser Lys Ser Arg Thr His Lys Asp 405 410 415 Leu Gln Asn Leu Leu Ile Lys Asp Asn Tyr Ser Ile Gln Glu Ile Glu 420 425 430 Arg Ala Ile Gln Thr Asn Leu Glu Leu Gly Leu Ile Thr Lys Asn Glu 435 440 445 Ser Asp Glu Ile Val Ile Pro Thr Glu His Ile Cys Ile Ala Arg Arg 450 455 460 Leu Leu Ile Leu Asp Ile Ala Ala Asn Tyr Ser Cys Ser Ile Thr Gln 465 470 475 480 Ala Glu Lys Ser Ser Gln Lys Tyr Leu Leu Val Pro Gly Asn Gly Ala 485 490 495 Asn Lys Gly Ile Lys Glu Ser Lys Leu Ala Glu Leu Ala Phe Asp Leu 500 505 510 Cys Lys Asp Ile Asn Ile Thr Pro Thr Glu Phe Ile Glu Asp Ile Glu 515 520 525 Trp Leu Leu Asp Glu Gly Val Ile Lys Arg Phe 530 535 151783DNABacillus stearothermophilus G668 151atgtacaatt atttattaaa cgaaaatgca gatataattt atgatggaaa ggttatactc 60acaaaagaac aagttgttga agcaattatt ataacaaata caaacttaaa gaaacttaat 120gacattacga aagagtctgg tgtcgaagtg tttgaagcat taggaatgag aaacctaagt 180ggttttattg gtgagttttt cgtaagcagt ctcgaacaag tatcaaataa gaacttagtt 240aaaaatccac atcaagatgg atatccagat ttgttgctcg tagattctcc taaagctgcc 300tcatacttta attcaatagt cgaaatagtt gatggaaaat tatatccaaa agaaaaaagt 360ctgtttagcc catttaaata tggtggatta gaggtaaaag ccacttgtgg ttctacacct 420tcagcaaaag ttatgcctaa gccattgatt ggcgagcaga gaattcacat cttaactgga 480ttagattgga aggcccatca tagaggtact aacaatctaa taggaatata ttgggatttt 540ttagatgagt taccaaccat ttgcgctgta ttttatagaa acgacctaac cgaagatgat 600tggggaaaaa ttgttcgccc taaagaaggt gggggaagaa ccacaagtgt atccattatg 660aactcaaagg gtgtcaaaaa aatgtgcaag aactggattg ctattattga taacgaagat 720tatataaacg cattttctaa taaaaaatgg ataggatata atgtaaaaaa ctcatcaaat 780tag 783152260PRTBacillus stearothermophilus G668 152Met Tyr Asn Tyr Leu Leu Asn Glu Asn Ala Asp Ile Ile Tyr Asp Gly 1 5 10 15 Lys Val Ile Leu Thr Lys Glu Gln Val Val Glu Ala Ile Ile Ile Thr 20 25 30 Asn Thr Asn Leu Lys Lys Leu Asn Asp Ile Thr Lys Glu Ser Gly Val 35 40 45 Glu Val Phe Glu Ala Leu Gly Met Arg Asn Leu Ser Gly Phe Ile Gly 50 55 60 Glu Phe Phe Val Ser Ser Leu Glu Gln Val Ser Asn Lys Asn Leu Val 65 70 75 80 Lys Asn Pro His Gln Asp Gly Tyr Pro Asp Leu Leu Leu Val Asp Ser 85 90 95 Pro Lys Ala Ala Ser Tyr Phe Asn Ser Ile Val Glu Ile Val Asp Gly 100 105 110 Lys Leu Tyr Pro Lys Glu Lys Ser Leu Phe Ser Pro Phe Lys Tyr Gly 115 120 125 Gly Leu Glu Val Lys Ala Thr Cys Gly Ser Thr Pro Ser Ala Lys Val 130 135 140 Met Pro Lys Pro Leu Ile Gly Glu Gln Arg Ile His Ile Leu Thr Gly 145 150 155 160 Leu Asp Trp Lys Ala His His Arg Gly Thr Asn Asn Leu Ile Gly Ile 165 170 175 Tyr Trp Asp Phe Leu Asp Glu Leu Pro Thr Ile Cys Ala Val Phe Tyr 180 185 190 Arg Asn Asp Leu Thr Glu Asp Asp Trp Gly Lys Ile Val Arg Pro Lys 195 200 205 Glu Gly Gly Gly Arg Thr Thr Ser Val Ser Ile Met Asn Ser Lys Gly 210 215 220 Val Lys Lys Met Cys Lys Asn Trp Ile Ala Ile Ile Asp Asn Glu Asp 225 230 235 240 Tyr Ile Asn Ala Phe Ser Asn Lys Lys Trp Ile Gly Tyr Asn Val Lys 245 250 255 Asn Ser Ser Asn 260 1532640DNABacillus stearothermophilus F 153atgttgagta ccataacaag gttgcctgta tatgatgatg atgaagtagg agttttccat 60cctatttgcg agtctgcatt aaatcaggct ctgtctaatc ttggcttgga taaagagttt 120aaagttttac atcatgaagc cgttggtagt cttgaagcag attttgcatt aatacgggag 180tcaacaagaa aatatgtttt attcattgag gtgaagagga aaccagccgc tgtaagtagt 240acaagatata gaatacaagc acagtcatat gttcaagaag caaaaacagc agtagaaaaa 300ccttattatg ctattactaa tttagaagta ctggatattt ttaagtatga tagtagcagg 360ccttcagtta ctcagcaaat aattgaacca agtccagttc gtataggtac attttccgac 420aatcccgtag aattttttaa taacttagtt aaaacttttg aggatattat cactattgtt 480gtaaatgaca gtggtacata caaagaacta actggaagtt ttattccatt actagaaaat 540aataaaacta atcaacaacg ttggcatcaa agtttattag ttgccggata tgaatatatt 600agaggtgtaa tgcaagcaag taaaagaaat atgacatgga aagcagcctt gaattacaaa 660aatagaccta ataaattagt tgagaatatt cgatcagtaa attttagttc attagttgtt 720ccaccgttac ctgctagtaa agatagtgaa atttggaata catcgatgtt agaggacttg 780gtagagttag gaaaaaaaac aatgagtggt gatggactgg ctgaattagt tcattcaatt 840gctgtttccg ggagggagca cgagggttta gtacctacag acttagagtt ggctaatatt 900ttggctattt tatcaaaata tgttctcggt agagagttaa atgaaaatga gattatatgc 960gatccagcag cgggaagtgg aaatttgtta gcagccatca gggcaggatt tgatacaata 1020aatccaaaac aattatgggc aaatgataaa gagcagttat ttcttgaact actttctatt 1080agattaggtt tgatgtttcc actaatagtc tcacctacaa actcaccttt agttacagga 1140aaagatattt gtgaccttaa taagaacgat tttacgaatg ttagtgtagt tcttatgaat 1200cctccatatg tttcaggggt aaaggaccct ataactaaga aaaaagttgc caaacgtata 1260tttgatatta gtggaacaat gtccaagact aatattggac aagtaggaat agaagcacca 1320ttcttagaac taattacaaa cttggtgaaa gataatacaa ttataggtgt ggttttccca 1380aaacagtatt taaccgcgag aggtagagag gcagaagcat taagaaatta tttattaaat 1440gatttcggtt taaaccttat ttttatatat ccgagagaag gaatattcaa agatgtaaca 1500aaagatactg tagtactaat agggagaaag aataatcctt ctagcaaagt gaaagttata 1560aaaagcgaaa ttccacttgc agaaataaat cttacaaaat ttaaacaagg cttgaataat 1620ttaaaaacaa atagttcaat acattctctt gcatatggag ttgatgtaag ggaacttaat 1680gcaaatgaat tgcatcagaa agttaaagat ggatggagaa gtcttacaaa tgttggtcaa 1740aaaatagata attggattaa tagtactctt attccaatta gtgagaaatt atctggagtt 1800cataatttaa aaaaaggaag aattggaaat gcaggtgcct cggacttatt atttataaac 1860tcaaatcata aattatggga attggttaag catattattc caaaagattg gttatatcca 1920gcccttcgac ttgtaaaaga tattaataac gtatttgtta attcatctac tacggatgtt 1980cgttttcttg ccccatgtga aaaagcgttt caaacaggta caaaagaata tagtatttta 2040gaagaaatat tagatatata cgaagaagta aaagcagaat ccttagtaaa aacaaaacaa 2100ccaaagaaaa taaagactaa ggaagaattg cgaaagatat taaatagaga aagaaagaaa 2160ataactagtc cttatactat tttaattcct cgtaatatta gaagatatgc tagggtattt 2220attacaactg aaagtgcata catatcaaca aatgtaattg aggtaactgg tggaacaaag 2280gatcaaaagt ggatcacgtt ttcatggttg ttaagtatat tttcgcaact tcaattagaa 2340gtaatgtcaa aagagcaaga aggtgcaaga aagacagaag taggtagtat taaggattta 2400cttttaccta agttcgaaaa cattgataac aaaatagtcg aaaaactaat aaatgagact 2460gaaactagaa taggattttt ggatttatgt aatccatcaa ctactacaat tgataagtta 2520tgggctgaag ttttatcttc ttcgaaacca gaagagatac tgaatcaagc attgttttta 2580ttagaagaaa aagtgaatga gagatatcct gagtatttag tatcagatga tgatgaataa 264015426DNAunknownMethylophilus methylotrophus - recognition sequence for 5' to 3' direction 154tccracnnnn nnnnnnnnnn nnnnnn 2615526DNAunknownCorynebacterium striatum M82B - recognition sequence - 5' to 3' direction 155aaggagnnnn nnnnnnnnnn nnnnnn 26156879PRTBacillus stearothermophilus F 156Met Leu Ser Thr Ile Thr Arg Leu Pro Val Tyr Asp Asp Asp Glu Val 1 5 10 15 Gly Val Phe His Pro Ile Cys Glu Ser Ala Leu Asn Gln Ala Leu Ser 20 25 30 Asn Leu Gly Leu Asp Lys Glu Phe Lys Val Leu His His Glu Ala Val 35 40 45 Gly Ser Leu Glu Ala Asp Phe Ala Leu Ile Arg Glu Ser Thr Arg Lys 50 55 60 Tyr Val Leu Phe Ile Glu Val Lys Arg Lys Pro Ala Ala Val Ser Ser 65 70 75 80 Thr Arg Tyr Arg Ile Gln Ala Gln Ser Tyr Val Gln Glu Ala Lys Thr 85 90 95 Ala Val Glu Lys Pro Tyr Tyr Ala Ile Thr Asn Leu Glu Val Leu Asp 100 105 110 Ile Phe Lys Tyr Asp Ser Ser Arg Pro Ser Val Thr Gln Gln Ile Ile 115 120 125 Glu Pro Ser Pro Val Arg Ile Gly Thr Phe Ser Asp Asn Pro Val Glu 130 135 140 Phe Phe Asn Asn Leu Val Lys Thr Phe Glu Asp Ile Ile Thr Ile Val 145 150 155 160 Val Asn Asp Ser Gly Thr Tyr Lys Glu Leu Thr Gly Ser Phe Ile Pro 165 170 175 Leu Leu Glu Asn Asn Lys Thr Asn Gln Gln Arg Trp His Gln Ser Leu 180 185 190 Leu Val Ala Gly Tyr Glu Tyr Ile Arg Gly Val Met Gln Ala Ser Lys 195 200 205 Arg Asn Met Thr Trp Lys Ala Ala Leu Asn Tyr Lys Asn Arg Pro Asn 210 215 220 Lys Leu Val Glu Asn Ile Arg Ser Val Asn Phe Ser Ser Leu Val Val 225 230 235 240 Pro Pro Leu Pro Ala Ser Lys Asp Ser Glu Ile Trp Asn Thr Ser Met 245 250 255 Leu Glu Asp

Leu Val Glu Leu Gly Lys Lys Thr Met Ser Gly Asp Gly 260 265 270 Leu Ala Glu Leu Val His Ser Ile Ala Val Ser Gly Arg Glu His Glu 275 280 285 Gly Leu Val Pro Thr Asp Leu Glu Leu Ala Asn Ile Leu Ala Ile Leu 290 295 300 Ser Lys Tyr Val Leu Gly Arg Glu Leu Asn Glu Asn Glu Ile Ile Cys 305 310 315 320 Asp Pro Ala Ala Gly Ser Gly Asn Leu Leu Ala Ala Ile Arg Ala Gly 325 330 335 Phe Asp Thr Ile Asn Pro Lys Gln Leu Trp Ala Asn Asp Lys Glu Gln 340 345 350 Leu Phe Leu Glu Leu Leu Ser Ile Arg Leu Gly Leu Met Phe Pro Leu 355 360 365 Ile Val Ser Pro Thr Asn Ser Pro Leu Val Thr Gly Lys Asp Ile Cys 370 375 380 Asp Leu Asn Lys Asn Asp Phe Thr Asn Val Ser Val Val Leu Met Asn 385 390 395 400 Pro Pro Tyr Val Ser Gly Val Lys Asp Pro Ile Thr Lys Lys Lys Val 405 410 415 Ala Lys Arg Ile Phe Asp Ile Ser Gly Thr Met Ser Lys Thr Asn Ile 420 425 430 Gly Gln Val Gly Ile Glu Ala Pro Phe Leu Glu Leu Ile Thr Asn Leu 435 440 445 Val Lys Asp Asn Thr Ile Ile Gly Val Val Phe Pro Lys Gln Tyr Leu 450 455 460 Thr Ala Arg Gly Arg Glu Ala Glu Ala Leu Arg Asn Tyr Leu Leu Asn 465 470 475 480 Asp Phe Gly Leu Asn Leu Ile Phe Ile Tyr Pro Arg Glu Gly Ile Phe 485 490 495 Lys Asp Val Thr Lys Asp Thr Val Val Leu Ile Gly Arg Lys Asn Asn 500 505 510 Pro Ser Ser Lys Val Lys Val Ile Lys Ser Glu Ile Pro Leu Ala Glu 515 520 525 Ile Asn Leu Thr Lys Phe Lys Gln Gly Leu Asn Asn Leu Lys Thr Asn 530 535 540 Ser Ser Ile His Ser Leu Ala Tyr Gly Val Asp Val Arg Glu Leu Asn 545 550 555 560 Ala Asn Glu Leu His Gln Lys Val Lys Asp Gly Trp Arg Ser Leu Thr 565 570 575 Asn Val Gly Gln Lys Ile Asp Asn Trp Ile Asn Ser Thr Leu Ile Pro 580 585 590 Ile Ser Glu Lys Leu Ser Gly Val His Asn Leu Lys Lys Gly Arg Ile 595 600 605 Gly Asn Ala Gly Ala Ser Asp Leu Leu Phe Ile Asn Ser Asn His Lys 610 615 620 Leu Trp Glu Leu Val Lys His Ile Ile Pro Lys Asp Trp Leu Tyr Pro 625 630 635 640 Ala Leu Arg Leu Val Lys Asp Ile Asn Asn Val Phe Val Asn Ser Ser 645 650 655 Thr Thr Asp Val Arg Phe Leu Ala Pro Cys Glu Lys Ala Phe Gln Thr 660 665 670 Gly Thr Lys Glu Tyr Ser Ile Leu Glu Glu Ile Leu Asp Ile Tyr Glu 675 680 685 Glu Val Lys Ala Glu Ser Leu Val Lys Thr Lys Gln Pro Lys Lys Ile 690 695 700 Lys Thr Lys Glu Glu Leu Arg Lys Ile Leu Asn Arg Glu Arg Lys Lys 705 710 715 720 Ile Thr Ser Pro Tyr Thr Ile Leu Ile Pro Arg Asn Ile Arg Arg Tyr 725 730 735 Ala Arg Val Phe Ile Thr Thr Glu Ser Ala Tyr Ile Ser Thr Asn Val 740 745 750 Ile Glu Val Thr Gly Gly Thr Lys Asp Gln Lys Trp Ile Thr Phe Ser 755 760 765 Trp Leu Leu Ser Ile Phe Ser Gln Leu Gln Leu Glu Val Met Ser Lys 770 775 780 Glu Gln Glu Gly Ala Arg Lys Thr Glu Val Gly Ser Ile Lys Asp Leu 785 790 795 800 Leu Leu Pro Lys Phe Glu Asn Ile Asp Asn Lys Ile Val Glu Lys Leu 805 810 815 Ile Asn Glu Thr Glu Thr Arg Ile Gly Phe Leu Asp Leu Cys Asn Pro 820 825 830 Ser Thr Thr Thr Ile Asp Lys Leu Trp Ala Glu Val Leu Ser Ser Ser 835 840 845 Lys Pro Glu Glu Ile Leu Asn Gln Ala Leu Phe Leu Leu Glu Glu Lys 850 855 860 Val Asn Glu Arg Tyr Pro Glu Tyr Leu Val Ser Asp Asp Asp Glu 865 870 875 157903DNABacillus species 157atgattgaaa cggtgttaga gaaagttaca aataaaaaca attttgttac attacaaaat 60tatacggatt ttgctttata ttttttagag tatattcaga agaataaaca agctacaatt 120gtttcacaaa atgaacatgt atataacttt tatcaatata atagtgaagc gaattatcaa 180gtaactcgcc ctttcaattc aaaaatttta tattctcacc aagatttttt ggataaccta 240ggggaattca ataaaatatt gaaggatttg aaaagcgacc gtaatcatgc aaaaattttg 300gatagaagta ttattaatag aacaatttat acggtacaac aaacaatagg ttttgcattg 360gacggtcttg acgcaaatag gacaaatgta gctcgaaaac tgaatggaga ctatttcgag 420cagttaattt tattactgct gcgagaaatc ggtgctcccg cgaataacgg ggttgtaaaa 480gtccctgtaa atatggaaga caaacaacta ttcaatatga gttatcaaca cgatcttata 540cttaaagaca aaaaaggcga ggtaaaattg attggttctg ttaaaacaac ttcaaaggat 600agaattggaa agatttttgt cgataagttt ctatattcga aattaacgga aacaacagta 660ccccacattg caattttctt acatgatgtt caaagaaaga ggaataaaga tccgcaaaaa 720ttcgggataa atggcacctt tttagcagga cattttaaag gttacacggt taaattaaat 780ccccttgatg gagtgtatta tttcgaccca cgcccacaaa tgcaaactga tgttctattg 840agtgaacata tacaaacgtt cgaccatttg ctttgcgatg atatttggag ttatgttgat 900tga 903158300PRTBacillus species 158Met Ile Glu Thr Val Leu Glu Lys Val Thr Asn Lys Asn Asn Phe Val 1 5 10 15 Thr Leu Gln Asn Tyr Thr Asp Phe Ala Leu Tyr Phe Leu Glu Tyr Ile 20 25 30 Gln Lys Asn Lys Gln Ala Thr Ile Val Ser Gln Asn Glu His Val Tyr 35 40 45 Asn Phe Tyr Gln Tyr Asn Ser Glu Ala Asn Tyr Gln Val Thr Arg Pro 50 55 60 Phe Asn Ser Lys Ile Leu Tyr Ser His Gln Asp Phe Leu Asp Asn Leu 65 70 75 80 Gly Glu Phe Asn Lys Ile Leu Lys Asp Leu Lys Ser Asp Arg Asn His 85 90 95 Ala Lys Ile Leu Asp Arg Ser Ile Ile Asn Arg Thr Ile Tyr Thr Val 100 105 110 Gln Gln Thr Ile Gly Phe Ala Leu Asp Gly Leu Asp Ala Asn Arg Thr 115 120 125 Asn Val Ala Arg Lys Leu Asn Gly Asp Tyr Phe Glu Gln Leu Ile Leu 130 135 140 Leu Leu Leu Arg Glu Ile Gly Ala Pro Ala Asn Asn Gly Val Val Lys 145 150 155 160 Val Pro Val Asn Met Glu Asp Lys Gln Leu Phe Asn Met Ser Tyr Gln 165 170 175 His Asp Leu Ile Leu Lys Asp Lys Lys Gly Glu Val Lys Leu Ile Gly 180 185 190 Ser Val Lys Thr Thr Ser Lys Asp Arg Ile Gly Lys Ile Phe Val Asp 195 200 205 Lys Phe Leu Tyr Ser Lys Leu Thr Glu Thr Thr Val Pro His Ile Ala 210 215 220 Ile Phe Leu His Asp Val Gln Arg Lys Arg Asn Lys Asp Pro Gln Lys 225 230 235 240 Phe Gly Ile Asn Gly Thr Phe Leu Ala Gly His Phe Lys Gly Tyr Thr 245 250 255 Val Lys Leu Asn Pro Leu Asp Gly Val Tyr Tyr Phe Asp Pro Arg Pro 260 265 270 Gln Met Gln Thr Asp Val Leu Leu Ser Glu His Ile Gln Thr Phe Asp 275 280 285 His Leu Leu Cys Asp Asp Ile Trp Ser Tyr Val Asp 290 295 300 1591398DNABacillus thermosphaericus 159atgaaacgaa ttttatactt gctaactgaa gaaagaccta aaataaatat tatacaccaa 60attattaatc ttgaatataa agcaacatta cactttggtg cgaaaatagt accagttatg 120aatgaggaaa ataagtttac atttatttat cacgtaaaag ggattgaagt tgagggattt 180gatgcagtct taataaaaat tgtttcaggt catagttcat tcgttgatta ccttgttttt 240gatagcaacg atctaaaacc tgaaaaaaat actattactc tatttgattt agatcaatat 300gaactagatt taagttatta ttttggaaaa ggatggattg tacgaatccc aagtccttcc 360gacttgccaa aatatgtggt tgaggaaaca aaaacagacg atcatgaatc gagaaatact 420aatgcttatc aacgttcttc aaaatttgtt ttttgtgagc tctattatgg taaagaagta 480aaaaaataca tgttatatga tatttctgat gggagaactt tatctgggac tgacactcat 540aattttggta tgcgtatgtt agttacgaat aatgttaact tagtaggtgt tccaaatatg 600tacttaccat ttacagatat aaaggagttt atcaatgaaa aaaatagaat agctgataat 660ggaccaagtc ataatgtgcc tattcgactt aaattagata aggaaaagaa tgttatttat 720atttcagcca aacttgataa aggtaatggg aaaaataaaa ataaaatttc aaatgatcca 780aatattggag cggtagctat tatttctgca acattacgta atttaaattg gaaaggtgat 840attgaaatta taaatcataa ccttttacct tcaagtatct catcacgtag caatggaaat 900aagctattat atataatgaa aaaattagga gttcgtttta ataacattaa tgtaaattgg 960aataatatta aaaataatat taattatttt ttctataata taacttctga gaaaatagtt 1020tcaatatact atcaccttta tgttgaggat aaattaagta atgcaagagt aatctttgat 1080aatcatgctg gctgtggtaa aagttatttt agaacactta ataataaaat tattccagtt 1140ggtaaagaaa ttcccttacc agacttagta atttttgatt cagatcaaaa tattgtaaaa 1200gttattgagg ctgaaaaagc agaaaatgtt tataatggtg ttgaacagct tagtacattt 1260gacaaattta tagagtctta tattaataaa tattacccag gagccgcagt agaatgttct 1320gtaattactt gggggaaatc aagcaatcca tatgtaagtt tttatttaga taaagatgga 1380agcgctgttt ttctgtaa 1398160465PRTBacillus thermosphaericus 160Met Lys Arg Ile Leu Tyr Leu Leu Thr Glu Glu Arg Pro Lys Ile Asn 1 5 10 15 Ile Ile His Gln Ile Ile Asn Leu Glu Tyr Lys Ala Thr Leu His Phe 20 25 30 Gly Ala Lys Ile Val Pro Val Met Asn Glu Glu Asn Lys Phe Thr Phe 35 40 45 Ile Tyr His Val Lys Gly Ile Glu Val Glu Gly Phe Asp Ala Val Leu 50 55 60 Ile Lys Ile Val Ser Gly His Ser Ser Phe Val Asp Tyr Leu Val Phe 65 70 75 80 Asp Ser Asn Asp Leu Lys Pro Glu Lys Asn Thr Ile Thr Leu Phe Asp 85 90 95 Leu Asp Gln Tyr Glu Leu Asp Leu Ser Tyr Tyr Phe Gly Lys Gly Trp 100 105 110 Ile Val Arg Ile Pro Ser Pro Ser Asp Leu Pro Lys Tyr Val Val Glu 115 120 125 Glu Thr Lys Thr Asp Asp His Glu Ser Arg Asn Thr Asn Ala Tyr Gln 130 135 140 Arg Ser Ser Lys Phe Val Phe Cys Glu Leu Tyr Tyr Gly Lys Glu Val 145 150 155 160 Lys Lys Tyr Met Leu Tyr Asp Ile Ser Asp Gly Arg Thr Leu Ser Gly 165 170 175 Thr Asp Thr His Asn Phe Gly Met Arg Met Leu Val Thr Asn Asn Val 180 185 190 Asn Leu Val Gly Val Pro Asn Met Tyr Leu Pro Phe Thr Asp Ile Lys 195 200 205 Glu Phe Ile Asn Glu Lys Asn Arg Ile Ala Asp Asn Gly Pro Ser His 210 215 220 Asn Val Pro Ile Arg Leu Lys Leu Asp Lys Glu Lys Asn Val Ile Tyr 225 230 235 240 Ile Ser Ala Lys Leu Asp Lys Gly Asn Gly Lys Asn Lys Asn Lys Ile 245 250 255 Ser Asn Asp Pro Asn Ile Gly Ala Val Ala Ile Ile Ser Ala Thr Leu 260 265 270 Arg Asn Leu Asn Trp Lys Gly Asp Ile Glu Ile Ile Asn His Asn Leu 275 280 285 Leu Pro Ser Ser Ile Ser Ser Arg Ser Asn Gly Asn Lys Leu Leu Tyr 290 295 300 Ile Met Lys Lys Leu Gly Val Arg Phe Asn Asn Ile Asn Val Asn Trp 305 310 315 320 Asn Asn Ile Lys Asn Asn Ile Asn Tyr Phe Phe Tyr Asn Ile Thr Ser 325 330 335 Glu Lys Ile Val Ser Ile Tyr Tyr His Leu Tyr Val Glu Asp Lys Leu 340 345 350 Ser Asn Ala Arg Val Ile Phe Asp Asn His Ala Gly Cys Gly Lys Ser 355 360 365 Tyr Phe Arg Thr Leu Asn Asn Lys Ile Ile Pro Val Gly Lys Glu Ile 370 375 380 Pro Leu Pro Asp Leu Val Ile Phe Asp Ser Asp Gln Asn Ile Val Lys 385 390 395 400 Val Ile Glu Ala Glu Lys Ala Glu Asn Val Tyr Asn Gly Val Glu Gln 405 410 415 Leu Ser Thr Phe Asp Lys Phe Ile Glu Ser Tyr Ile Asn Lys Tyr Tyr 420 425 430 Pro Gly Ala Ala Val Glu Cys Ser Val Ile Thr Trp Gly Lys Ser Ser 435 440 445 Asn Pro Tyr Val Ser Phe Tyr Leu Asp Lys Asp Gly Ser Ala Val Phe 450 455 460 Leu 465 161495DNABacillus thermoglucosidasius 161atgaaaataa cagagggaat cgtccatgtt gcaatgcggc actttctaaa atcaaatggc 60tggaaattaa ttgctgggca ataccctggt ggaagcgatg acgaattgac tgcacttaat 120attgttgatc ctgtggtagc tcgtgataat agtcctgatc ctcgccgtca tagtttaggt 180aaaattgttc ctgacctaat agcttataaa aacgatgatt tactcgttat tgaagcaaag 240ccgaaatatt cgcaggatga tagggataaa ttactttact tgctttcaga aagaaaacat 300gacttttacg cggctttaga aaaattcgct actgaaagga atcacccaga actactgccg 360gtatctaagc tgaatattat acctgggtta gcgttttccg cttcagaaaa caaattcaaa 420aaggatcccg gattcgttta cataagagta tctgggatct ttgaagcatt tatggagggc 480tatgattggg ggtga 495162164PRTBacillus thermoglucosidasius 162Met Lys Ile Thr Glu Gly Ile Val His Val Ala Met Arg His Phe Leu 1 5 10 15 Lys Ser Asn Gly Trp Lys Leu Ile Ala Gly Gln Tyr Pro Gly Gly Ser 20 25 30 Asp Asp Glu Leu Thr Ala Leu Asn Ile Val Asp Pro Val Val Ala Arg 35 40 45 Asp Asn Ser Pro Asp Pro Arg Arg His Ser Leu Gly Lys Ile Val Pro 50 55 60 Asp Leu Ile Ala Tyr Lys Asn Asp Asp Leu Leu Val Ile Glu Ala Lys 65 70 75 80 Pro Lys Tyr Ser Gln Asp Asp Arg Asp Lys Leu Leu Tyr Leu Leu Ser 85 90 95 Glu Arg Lys His Asp Phe Tyr Ala Ala Leu Glu Lys Phe Ala Thr Glu 100 105 110 Arg Asn His Pro Glu Leu Leu Pro Val Ser Lys Leu Asn Ile Ile Pro 115 120 125 Gly Leu Ala Phe Ser Ala Ser Glu Asn Lys Phe Lys Lys Asp Pro Gly 130 135 140 Phe Val Tyr Ile Arg Val Ser Gly Ile Phe Glu Ala Phe Met Glu Gly 145 150 155 160 Tyr Asp Trp Gly 163987DNABacillus thermoglucosidasius 163atgcagatcg aacaattaat gaaaagtctc acaatctatt ttgacgacat acaagagggt 60ttatggttta aaaacttgca tcctctatta gaatccgcgt ctcttgaggc gattactgga 120tccctaaaaa gaaatccaaa cttagctgat gttttaaaat atgatagacc cgatatcatt 180cttaccttga atcaaacacc catattagta atagagcgaa caattgaggt tccaagtggg 240cataatgtcg gacaaagata tgggagatta gccgcagcat cggaagcagg agttccttta 300gtctactttg gtccttacgc tgccagaaaa catggtgggg ctactgaagg accacgatac 360atgaacttgc gtttatttta tgccctggat gtaatgcaaa aggtaaacgg ttctgctatt 420accactataa attggcctgt agatcagaat ttcgaaatac tccaagatcc atctaaagat 480aagagaatga aggagtattt agaaatgttc ttcgataatc ttttgaaata cggaatagcc 540ggtataaatt tagcgattag aaattcctct tttcaagctg agcaattagc tgaaagagaa 600aaatttgtgg aaactatgat aactaaccct gaacaatacg atgtcccgcc cgattcggtc 660caaattctta atgctgaaag gttcttcaat gaattaggta tatcagaaaa taagagaata 720atctgtgatg aggttgtttt atatcaagta ggaatgacat acgtcagatc agacccatat 780actggaatgg cccttttata taagtatctt tatatacttg ggagcgaacg aaatagatgt 840cttattttaa agttccctaa tattacaact gatatgtgga aaaaggtggc ttttggaagt 900agagagcgga aagacgtaag aatctaccga agtgtctcag atggaatatt gtttgcagat 960ggttatttat caaaagaaga gttgtaa 987164328PRTBacillus thermoglucosidasius 164Met Gln Ile Glu Gln Leu Met Lys Ser Leu Thr Ile Tyr Phe Asp Asp 1 5 10 15 Ile Gln Glu Gly Leu Trp Phe Lys Asn Leu His Pro Leu Leu Glu Ser 20 25 30 Ala Ser Leu Glu Ala Ile Thr Gly Ser Leu Lys Arg Asn Pro Asn Leu 35 40 45 Ala Asp Val Leu Lys Tyr Asp Arg Pro Asp Ile Ile Leu Thr Leu Asn 50 55 60 Gln Thr Pro Ile Leu Val Ile Glu Arg Thr Ile Glu Val Pro Ser Gly 65 70 75 80 His Asn Val Gly Gln Arg Tyr Gly Arg Leu Ala Ala Ala Ser Glu Ala 85 90 95 Gly Val Pro Leu Val Tyr Phe Gly Pro Tyr Ala Ala Arg Lys His Gly 100

105 110 Gly Ala Thr Glu Gly Pro Arg Tyr Met Asn Leu Arg Leu Phe Tyr Ala 115 120 125 Leu Asp Val Met Gln Lys Val Asn Gly Ser Ala Ile Thr Thr Ile Asn 130 135 140 Trp Pro Val Asp Gln Asn Phe Glu Ile Leu Gln Asp Pro Ser Lys Asp 145 150 155 160 Lys Arg Met Lys Glu Tyr Leu Glu Met Phe Phe Asp Asn Leu Leu Lys 165 170 175 Tyr Gly Ile Ala Gly Ile Asn Leu Ala Ile Arg Asn Ser Ser Phe Gln 180 185 190 Ala Glu Gln Leu Ala Glu Arg Glu Lys Phe Val Glu Thr Met Ile Thr 195 200 205 Asn Pro Glu Gln Tyr Asp Val Pro Pro Asp Ser Val Gln Ile Leu Asn 210 215 220 Ala Glu Arg Phe Phe Asn Glu Leu Gly Ile Ser Glu Asn Lys Arg Ile 225 230 235 240 Ile Cys Asp Glu Val Val Leu Tyr Gln Val Gly Met Thr Tyr Val Arg 245 250 255 Ser Asp Pro Tyr Thr Gly Met Ala Leu Leu Tyr Lys Tyr Leu Tyr Ile 260 265 270 Leu Gly Ser Glu Arg Asn Arg Cys Leu Ile Leu Lys Phe Pro Asn Ile 275 280 285 Thr Thr Asp Met Trp Lys Lys Val Ala Phe Gly Ser Arg Glu Arg Lys 290 295 300 Asp Val Arg Ile Tyr Arg Ser Val Ser Asp Gly Ile Leu Phe Ala Asp 305 310 315 320 Gly Tyr Leu Ser Lys Glu Glu Leu 325 165594DNAEscherichia coli 165atgaatattg gtttataccc aaatgatagt agagattggg gagaagacga ttggcatcaa 60tttttgcaag aattagttaa taataattta gtgtcatatg agcagatcac ttctctcgtt 120ttggggcatt taaacccatc tcaagttggt acatcaatag cctctaaaaa aacatttcag 180gcgcattatc ctcctcgtca atgttgggct gctgttcgtt cttggcattt tgagcagtcg 240gggcgatgca tcgactgtgg aactcgcctt gaattacagg cagatcatgt gcttccgcga 300gaattactag gtgatgaagc tgatcggctt gataatatgg ctttgagatg tcgaaggtgc 360aacgttataa gaaggccaag tcatagaaac ggtggaatag ctcatcttac taccgaatca 420gcactaatgt ggttgctctt tactcgtcag cctacaaatt atcaaacata tcgagatttg 480tgtcgtgcat atggaatgac tatggcaagt atccgtttcg aagaagcatg ggctatggca 540agatggctgg aaagagaagg tttgtattat atagacgaaa cttctatttt ttga 594166197PRTEscherichia coli 166Met Asn Ile Gly Leu Tyr Pro Asn Asp Ser Arg Asp Trp Gly Glu Asp 1 5 10 15 Asp Trp His Gln Phe Leu Gln Glu Leu Val Asn Asn Asn Leu Val Ser 20 25 30 Tyr Glu Gln Ile Thr Ser Leu Val Leu Gly His Leu Asn Pro Ser Gln 35 40 45 Val Gly Thr Ser Ile Ala Ser Lys Lys Thr Phe Gln Ala His Tyr Pro 50 55 60 Pro Arg Gln Cys Trp Ala Ala Val Arg Ser Trp His Phe Glu Gln Ser 65 70 75 80 Gly Arg Cys Ile Asp Cys Gly Thr Arg Leu Glu Leu Gln Ala Asp His 85 90 95 Val Leu Pro Arg Glu Leu Leu Gly Asp Glu Ala Asp Arg Leu Asp Asn 100 105 110 Met Ala Leu Arg Cys Arg Arg Cys Asn Val Ile Arg Arg Pro Ser His 115 120 125 Arg Asn Gly Gly Ile Ala His Leu Thr Thr Glu Ser Ala Leu Met Trp 130 135 140 Leu Leu Phe Thr Arg Gln Pro Thr Asn Tyr Gln Thr Tyr Arg Asp Leu 145 150 155 160 Cys Arg Ala Tyr Gly Met Thr Met Ala Ser Ile Arg Phe Glu Glu Ala 165 170 175 Trp Ala Met Ala Arg Trp Leu Glu Arg Glu Gly Leu Tyr Tyr Ile Asp 180 185 190 Glu Thr Ser Ile Phe 195 167510DNAFusobacterium nucleatum 4H 167atgagtgata taaaaaaaat aacagatgtt gagtataaaa tggtaatttc gctttatcct 60atttggaaag aactaaatag ttctataaaa agcatatatt ctcgtggtgt taattttcat 120gaagtttttt ctgaatttat tgtatgctat ataaataatt actatcatag tcttgggagt 180ggttcagaag atgcttatac aagtgatatg aaaaaaaagg ttcaagtaaa agctagttct 240aattttaata gtgatttaac tagttttggt ccaactagtg aatttgatat tctagaattt 300gctcgtttaa atcaagaaga aaataaatta tatctatata aaattccaat agataattta 360tataatataa atgtaaattc taacgaaaca tttaaagaac aacaacaaag tggaagaaga 420ccaagatttt ctattataga aaaatatata aaagaatata atctaaagca ctatgcagtt 480gttgatatga taactggtct ttatttttaa 510168169PRTFusobacterium nucleatum 4H 168Met Ser Asp Ile Lys Lys Ile Thr Asp Val Glu Tyr Lys Met Val Ile 1 5 10 15 Ser Leu Tyr Pro Ile Trp Lys Glu Leu Asn Ser Ser Ile Lys Ser Ile 20 25 30 Tyr Ser Arg Gly Val Asn Phe His Glu Val Phe Ser Glu Phe Ile Val 35 40 45 Cys Tyr Ile Asn Asn Tyr Tyr His Ser Leu Gly Ser Gly Ser Glu Asp 50 55 60 Ala Tyr Thr Ser Asp Met Lys Lys Lys Val Gln Val Lys Ala Ser Ser 65 70 75 80 Asn Phe Asn Ser Asp Leu Thr Ser Phe Gly Pro Thr Ser Glu Phe Asp 85 90 95 Ile Leu Glu Phe Ala Arg Leu Asn Gln Glu Glu Asn Lys Leu Tyr Leu 100 105 110 Tyr Lys Ile Pro Ile Asp Asn Leu Tyr Asn Ile Asn Val Asn Ser Asn 115 120 125 Glu Thr Phe Lys Glu Gln Gln Gln Ser Gly Arg Arg Pro Arg Phe Ser 130 135 140 Ile Ile Glu Lys Tyr Ile Lys Glu Tyr Asn Leu Lys His Tyr Ala Val 145 150 155 160 Val Asp Met Ile Thr Gly Leu Tyr Phe 165 169858DNAKluyvera ascorbata 169atgagcgtta ttccgtgtaa aaaggacctt cagctaaaaa aattgattga atcctatgca 60gaagccttga aagttgaggc ccataagcta ggagagcatg gattaactga agctgaattt 120tatgatagcg gcctctttcg gggggctatc gagcgaattc gaggacagtt ctccgcgacc 180atgcgggaga aaagaaattt cgttaagcat gttttaaatt acatgcagga taacgactac 240attgctgatt gggagtcggc tggtgaatcg aatcgccatg attatatggt aactctcaat 300tctgggcgca aagctgctat tgagctgaaa gggtgccttg atggcaataa cactaacatc 360tttgatcgcc cccctcaggc agaagaattt gttatctgga gtgtatgcac aaatcctggt 420gctgaccctc agcataatgt ttggtctggg cttcacacca gactaagtgc tgaaatcatt 480tcacgggagc aaaggattga tggaatggtc atttgggact gggcttgtgg aacagtcgga 540aggccatgcc ccaaaatagc aactgaacct gagcgggctg taacatttgg gccgttcaaa 600ttgccgccac catgtttgta tcttttacct tcgacgattc caagcccaag aaacaacccg 660tctccaagag ctcagcagat tgaagacgtg cagctaatca aagcgtttca cgattgtttt 720gggtgccggt ctgaagaagt taatttcgtt aactttgatg ttggttatca tggtaaagat 780accgtccgta aaacgactat cattcgaaac ggcatggtgg agcgtgaatc ggaaatgacg 840gcaataaggc ggtcttaa 858170285PRTKluyvera ascorbata 170Met Ser Val Ile Pro Cys Lys Lys Asp Leu Gln Leu Lys Lys Leu Ile 1 5 10 15 Glu Ser Tyr Ala Glu Ala Leu Lys Val Glu Ala His Lys Leu Gly Glu 20 25 30 His Gly Leu Thr Glu Ala Glu Phe Tyr Asp Ser Gly Leu Phe Arg Gly 35 40 45 Ala Ile Glu Arg Ile Arg Gly Gln Phe Ser Ala Thr Met Arg Glu Lys 50 55 60 Arg Asn Phe Val Lys His Val Leu Asn Tyr Met Gln Asp Asn Asp Tyr 65 70 75 80 Ile Ala Asp Trp Glu Ser Ala Gly Glu Ser Asn Arg His Asp Tyr Met 85 90 95 Val Thr Leu Asn Ser Gly Arg Lys Ala Ala Ile Glu Leu Lys Gly Cys 100 105 110 Leu Asp Gly Asn Asn Thr Asn Ile Phe Asp Arg Pro Pro Gln Ala Glu 115 120 125 Glu Phe Val Ile Trp Ser Val Cys Thr Asn Pro Gly Ala Asp Pro Gln 130 135 140 His Asn Val Trp Ser Gly Leu His Thr Arg Leu Ser Ala Glu Ile Ile 145 150 155 160 Ser Arg Glu Gln Arg Ile Asp Gly Met Val Ile Trp Asp Trp Ala Cys 165 170 175 Gly Thr Val Gly Arg Pro Cys Pro Lys Ile Ala Thr Glu Pro Glu Arg 180 185 190 Ala Val Thr Phe Gly Pro Phe Lys Leu Pro Pro Pro Cys Leu Tyr Leu 195 200 205 Leu Pro Ser Thr Ile Pro Ser Pro Arg Asn Asn Pro Ser Pro Arg Ala 210 215 220 Gln Gln Ile Glu Asp Val Gln Leu Ile Lys Ala Phe His Asp Cys Phe 225 230 235 240 Gly Cys Arg Ser Glu Glu Val Asn Phe Val Asn Phe Asp Val Gly Tyr 245 250 255 His Gly Lys Asp Thr Val Arg Lys Thr Thr Ile Ile Arg Asn Gly Met 260 265 270 Val Glu Arg Glu Ser Glu Met Thr Ala Ile Arg Arg Ser 275 280 285 1711023DNAMethylococcus capsulatus str. Bath 171atgacaaaag aagaatttga aagctatttg gacgacatcg cctctaagct cagggacgaa 60gccagaaaga cgccatttgc cgcagccaag cagttcgagc aacgtgttcg ggaaatcacc 120aaggaaacga tccaagctcc cgggatcgag atcgatttca acccacaccc tcaagcattc 180cccgacatag aaatcggtca gttcggaatt gaggtgaaat tcacgacaaa cgacgaatgg 240aggagcgtcg ccaacagcgt gctggaaacc aaccgtatcg aatccgtgca gcacgtgtac 300atcatgttcg gaaagatggg tggcaatccg gacgtgagat ggggtgaata cgagaaatgc 360gtcatgcatg tcagaacatc ccacgtccct cgcttcgagg tgcagatcga tgccactcga 420tccttattcg agatcatggg catttcttac gatcaattcc gggtgctcga aatgcacgag 480aagatgcagt acatccggaa atacgcaaga agcaggctga agaacggaga acgcttatgg 540tggctggaag attcgcccgg cgaagcccat accttgccta tgcaagctcg actattcact 600gagctagagc agtccgagaa gattcgactt cgcgccgaag caatcctact ttgtcctcaa 660atcgttcaat ctggcagagc ccggcataag tacgatgacg tcgcgttatt catgctgacc 720tatcacggcg tgatctgcca tcagaccaga gatatgttct ctgccggtag cgttggaaac 780ccggagaatg acgataacgg cggactctac atcgcgcgca tgctcaagct gatggaagcc 840gagttggaga aggcagcggc gcgcatggat gccgcgctgt ttgaggaata ttggggcgtg 900gctgtcccac ctgaggaaag aatagcggaa tggctgcgtc gcgcagacaa gttcgcgtcg 960ggaatttgga agccatctga agagttgttc gatggtagat acgctcagcc aagaggagcg 1020tag 1023172340PRTMethylococcus capsulatus str. Bath 172Met Thr Lys Glu Glu Phe Glu Ser Tyr Leu Asp Asp Ile Ala Ser Lys 1 5 10 15 Leu Arg Asp Glu Ala Arg Lys Thr Pro Phe Ala Ala Ala Lys Gln Phe 20 25 30 Glu Gln Arg Val Arg Glu Ile Thr Lys Glu Thr Ile Gln Ala Pro Gly 35 40 45 Ile Glu Ile Asp Phe Asn Pro His Pro Gln Ala Phe Pro Asp Ile Glu 50 55 60 Ile Gly Gln Phe Gly Ile Glu Val Lys Phe Thr Thr Asn Asp Glu Trp 65 70 75 80 Arg Ser Val Ala Asn Ser Val Leu Glu Thr Asn Arg Ile Glu Ser Val 85 90 95 Gln His Val Tyr Ile Met Phe Gly Lys Met Gly Gly Asn Pro Asp Val 100 105 110 Arg Trp Gly Glu Tyr Glu Lys Cys Val Met His Val Arg Thr Ser His 115 120 125 Val Pro Arg Phe Glu Val Gln Ile Asp Ala Thr Arg Ser Leu Phe Glu 130 135 140 Ile Met Gly Ile Ser Tyr Asp Gln Phe Arg Val Leu Glu Met His Glu 145 150 155 160 Lys Met Gln Tyr Ile Arg Lys Tyr Ala Arg Ser Arg Leu Lys Asn Gly 165 170 175 Glu Arg Leu Trp Trp Leu Glu Asp Ser Pro Gly Glu Ala His Thr Leu 180 185 190 Pro Met Gln Ala Arg Leu Phe Thr Glu Leu Glu Gln Ser Glu Lys Ile 195 200 205 Arg Leu Arg Ala Glu Ala Ile Leu Leu Cys Pro Gln Ile Val Gln Ser 210 215 220 Gly Arg Ala Arg His Lys Tyr Asp Asp Val Ala Leu Phe Met Leu Thr 225 230 235 240 Tyr His Gly Val Ile Cys His Gln Thr Arg Asp Met Phe Ser Ala Gly 245 250 255 Ser Val Gly Asn Pro Glu Asn Asp Asp Asn Gly Gly Leu Tyr Ile Ala 260 265 270 Arg Met Leu Lys Leu Met Glu Ala Glu Leu Glu Lys Ala Ala Ala Arg 275 280 285 Met Asp Ala Ala Leu Phe Glu Glu Tyr Trp Gly Val Ala Val Pro Pro 290 295 300 Glu Glu Arg Ile Ala Glu Trp Leu Arg Arg Ala Asp Lys Phe Ala Ser 305 310 315 320 Gly Ile Trp Lys Pro Ser Glu Glu Leu Phe Asp Gly Arg Tyr Ala Gln 325 330 335 Pro Arg Gly Ala 340 1731542DNANeisseria cinerea 173atgaaaataa ataaatttaa tttagaaaaa attttaaata aatttatatg cggtgattct 60ttgcaaaaaa tgaaaaaatt acctagtaaa tcaatcgatt taatttttac ttcccctcct 120tataatttaa aaaattcaac tggtaatgga atgaaagatg gtagaggcgg aaaatggtca 180aatgccagat taattgaagg gtatgacaac catgatgatt gtatgccaca tgatgagtat 240gtgaaatggc aacgtaaatg tttaaaagaa atgcttcgtc tgataaaaga tgatggtgct 300attttttata atcataaatg gagagtacaa aatggtctat tacaagatag agcagacatt 360gtaaaaggct ttcctgttcg ccaaattatt atttggaaaa gaaagggagg aattaatttt 420aatcctggat attttttgcc aacttatgaa gtaatttatt taatttgcaa gaaacctttt 480aaattggcaa aaggtgcaaa ttcatttgga gatatttggg aattcacgca agatatgaat 540aatgaacatc ctgcaccatt tcctttagaa ttagctaagc gagttgtaca aagtacaaat 600gctcaaatag tgcttgatcc atttatggga agtggaacaa ctgctattgc agcagcacta 660ttagacagaa agtttattgg cattgaactt tcatctgaat atgtcaagat atctaaaaaa 720agatataaca atatttttgg taatttattt ggagtagata tgaaaacttt tacaaaagaa 780tctttaattc aagagctaaa ggaaattaaa aataaaggtc cggttcttaa taacagagga 840agtaacaatg gggcttccgg gaatgtttta gaagatttgc tgggaattga agaaaataat 900ctccctttag caaatgctgc agaatgggaa ataaaaacca aaaaaagatc atccaattca 960ctggtaacac tatttcatgt tgaaccctct ccaaccgcat gtaaatttgt cccaaatata 1020ttattaccta aatacggatg gaaacataaa gaggctggaa aaaaatatcc tgacactgaa 1080aaaagtttta gacaaactat taagtgtggt ttattttctg atagaggatt ttctataaaa 1140cttaatgata gcgaagaaaa aattgaagta aattttagat atgatctaat agatcaaaag 1200cataatgaat ggaagcagga tatttctacc tttcagacat tggatacaat accatactgg 1260ggatttaatg atatatatca taaacttggt gcaaaattgc ataactgttt ttttgcgata 1320gtagatgttt gtaaaagagg agatgatgaa tattttacct atagtgaaat ttatatgctc 1380cgtaatttat cgaaagataa atttatatct gcaatccgag atggaaagat atatatagat 1440tttgatgcta gaacaggaca taatcatgga acaaaattta ggataaaaga aaaagatatt 1500tttgacctct atgaagaatg catcgaaata tcaaacttat ag 1542174513PRTNeisseria cinerea 174Met Lys Ile Asn Lys Phe Asn Leu Glu Lys Ile Leu Asn Lys Phe Ile 1 5 10 15 Cys Gly Asp Ser Leu Gln Lys Met Lys Lys Leu Pro Ser Lys Ser Ile 20 25 30 Asp Leu Ile Phe Thr Ser Pro Pro Tyr Asn Leu Lys Asn Ser Thr Gly 35 40 45 Asn Gly Met Lys Asp Gly Arg Gly Gly Lys Trp Ser Asn Ala Arg Leu 50 55 60 Ile Glu Gly Tyr Asp Asn His Asp Asp Cys Met Pro His Asp Glu Tyr 65 70 75 80 Val Lys Trp Gln Arg Lys Cys Leu Lys Glu Met Leu Arg Leu Ile Lys 85 90 95 Asp Asp Gly Ala Ile Phe Tyr Asn His Lys Trp Arg Val Gln Asn Gly 100 105 110 Leu Leu Gln Asp Arg Ala Asp Ile Val Lys Gly Phe Pro Val Arg Gln 115 120 125 Ile Ile Ile Trp Lys Arg Lys Gly Gly Ile Asn Phe Asn Pro Gly Tyr 130 135 140 Phe Leu Pro Thr Tyr Glu Val Ile Tyr Leu Ile Cys Lys Lys Pro Phe 145 150 155 160 Lys Leu Ala Lys Gly Ala Asn Ser Phe Gly Asp Ile Trp Glu Phe Thr 165 170 175 Gln Asp Met Asn Asn Glu His Pro Ala Pro Phe Pro Leu Glu Leu Ala 180 185 190 Lys Arg Val Val Gln Ser Thr Asn Ala Gln Ile Val Leu Asp Pro Phe 195 200 205 Met Gly Ser Gly Thr Thr Ala Ile Ala Ala Ala Leu Leu Asp Arg Lys 210 215 220 Phe Ile Gly Ile Glu Leu Ser Ser Glu Tyr Val Lys Ile Ser Lys Lys 225 230 235 240 Arg Tyr Asn Asn Ile Phe Gly Asn Leu Phe Gly Val Asp Met Lys Thr 245 250 255 Phe Thr Lys Glu Ser Leu Ile Gln Glu Leu Lys Glu Ile Lys Asn Lys 260 265 270 Gly Pro Val Leu Asn Asn Arg Gly Ser Asn Asn Gly Ala Ser Gly Asn 275 280 285 Val Leu Glu Asp Leu Leu Gly Ile Glu Glu Asn Asn Leu Pro Leu Ala 290 295 300 Asn Ala Ala Glu Trp Glu Ile Lys Thr Lys Lys Arg Ser Ser Asn Ser 305 310 315 320 Leu Val Thr Leu Phe His Val Glu Pro Ser Pro Thr Ala Cys Lys Phe 325

330 335 Val Pro Asn Ile Leu Leu Pro Lys Tyr Gly Trp Lys His Lys Glu Ala 340 345 350 Gly Lys Lys Tyr Pro Asp Thr Glu Lys Ser Phe Arg Gln Thr Ile Lys 355 360 365 Cys Gly Leu Phe Ser Asp Arg Gly Phe Ser Ile Lys Leu Asn Asp Ser 370 375 380 Glu Glu Lys Ile Glu Val Asn Phe Arg Tyr Asp Leu Ile Asp Gln Lys 385 390 395 400 His Asn Glu Trp Lys Gln Asp Ile Ser Thr Phe Gln Thr Leu Asp Thr 405 410 415 Ile Pro Tyr Trp Gly Phe Asn Asp Ile Tyr His Lys Leu Gly Ala Lys 420 425 430 Leu His Asn Cys Phe Phe Ala Ile Val Asp Val Cys Lys Arg Gly Asp 435 440 445 Asp Glu Tyr Phe Thr Tyr Ser Glu Ile Tyr Met Leu Arg Asn Leu Ser 450 455 460 Lys Asp Lys Phe Ile Ser Ala Ile Arg Asp Gly Lys Ile Tyr Ile Asp 465 470 475 480 Phe Asp Ala Arg Thr Gly His Asn His Gly Thr Lys Phe Arg Ile Lys 485 490 495 Glu Lys Asp Ile Phe Asp Leu Tyr Glu Glu Cys Ile Glu Ile Ser Asn 500 505 510 Leu 175660DNANocardia rubra 175atgggatttc ttgaagactg ggacctcagc tacgacgaga tcaacgagct tctcactgac 60aaccccagcc ttcgatcgtt cgtgatgggg tacgcagcgg agatcaagtg tcgcaacatg 120ttcttcgttg atcatccaca tatcaccaac atttacaagc ccgatgatca cgatcgcact 180gaaaagggcg actggatcat caactacaag ggacaccgga tcggggtcga ggtcaagagt 240ctccagacga actcactgcg gcttcgccga gatggcagtg tccgaccaaa ctaccagtgc 300gacgcttcgg atgcccgcac cgtgatcttc gctgacggta gcgaagttca tacgaccgct 360ctgttggtcg gagaatttga cgtagttgca gtcaatatcc atgcgttcga aaataagtgg 420gattttgcgt tcgctaagaa cgaggatctc atcacgatgg agggtgcgac caggggcgca 480gcgaaagact acaccgaact ccagaaacgc aatctcatca agactctcca accgatgcct 540atggacgtgc cagccccgta cactcgagat cccttcaaac tcttcgacga gatcatcgaa 600gagcgcatga agggtgagca gcctcagctc aaggcgaaga tcatcgaaga cgaagagtga 660176219PRTNocardia rubra 176Met Gly Phe Leu Glu Asp Trp Asp Leu Ser Tyr Asp Glu Ile Asn Glu 1 5 10 15 Leu Leu Thr Asp Asn Pro Ser Leu Arg Ser Phe Val Met Gly Tyr Ala 20 25 30 Ala Glu Ile Lys Cys Arg Asn Met Phe Phe Val Asp His Pro His Ile 35 40 45 Thr Asn Ile Tyr Lys Pro Asp Asp His Asp Arg Thr Glu Lys Gly Asp 50 55 60 Trp Ile Ile Asn Tyr Lys Gly His Arg Ile Gly Val Glu Val Lys Ser 65 70 75 80 Leu Gln Thr Asn Ser Leu Arg Leu Arg Arg Asp Gly Ser Val Arg Pro 85 90 95 Asn Tyr Gln Cys Asp Ala Ser Asp Ala Arg Thr Val Ile Phe Ala Asp 100 105 110 Gly Ser Glu Val His Thr Thr Ala Leu Leu Val Gly Glu Phe Asp Val 115 120 125 Val Ala Val Asn Ile His Ala Phe Glu Asn Lys Trp Asp Phe Ala Phe 130 135 140 Ala Lys Asn Glu Asp Leu Ile Thr Met Glu Gly Ala Thr Arg Gly Ala 145 150 155 160 Ala Lys Asp Tyr Thr Glu Leu Gln Lys Arg Asn Leu Ile Lys Thr Leu 165 170 175 Gln Pro Met Pro Met Asp Val Pro Ala Pro Tyr Thr Arg Asp Pro Phe 180 185 190 Lys Leu Phe Asp Glu Ile Ile Glu Glu Arg Met Lys Gly Glu Gln Pro 195 200 205 Gln Leu Lys Ala Lys Ile Ile Glu Asp Glu Glu 210 215 177675DNAShigella boydii 13 177atgagagaac cctcgattct agaaagatgg gaaataagcg aagaaaaatt aactgacttg 60gttgataaaa acccctctct tagaggaatg attttaggtt atgttgctga ggataaattt 120cacgagctat tccttgaaga tgaaagagta aaggaggttt ctaaagacga cgatcatgac 180agaaagaaaa aaggagatag aacctttatt tacaaaggta aaaaatttac agttgaagtt 240aaaagcttgc aaaccgcaat gtgcaagaaa aatgaagacg gaacttattc aggaaaagcc 300caagtagacg gcagtgatcg aagaatagta aaattcccag acaattcaga attaaatacg 360acgttactct tgaaaggaga gtttgatcta ttagccgtta attgctttgc ttttggtgaa 420ggatggaaat ttgcttttgc aaaaaattct gaccttccca cctcaacatt caaaaaatac 480acagaagaac aaaggaaaca acttattgcc tcactgattc ctgtaacttg gccaccaaag 540ccaccattca gtgatgaccc attccacctt ctggacgaga tgattgcagc gccagaagag 600gaaccggtga tagaagaaag tagtgaatta aaagaagtaa aagaagatat agatgtagtt 660aaagtgaaat cataa 675178224PRTShigella boydii 13 178Met Arg Glu Pro Ser Ile Leu Glu Arg Trp Glu Ile Ser Glu Glu Lys 1 5 10 15 Leu Thr Asp Leu Val Asp Lys Asn Pro Ser Leu Arg Gly Met Ile Leu 20 25 30 Gly Tyr Val Ala Glu Asp Lys Phe His Glu Leu Phe Leu Glu Asp Glu 35 40 45 Arg Val Lys Glu Val Ser Lys Asp Asp Asp His Asp Arg Lys Lys Lys 50 55 60 Gly Asp Arg Thr Phe Ile Tyr Lys Gly Lys Lys Phe Thr Val Glu Val 65 70 75 80 Lys Ser Leu Gln Thr Ala Met Cys Lys Lys Asn Glu Asp Gly Thr Tyr 85 90 95 Ser Gly Lys Ala Gln Val Asp Gly Ser Asp Arg Arg Ile Val Lys Phe 100 105 110 Pro Asp Asn Ser Glu Leu Asn Thr Thr Leu Leu Leu Lys Gly Glu Phe 115 120 125 Asp Leu Leu Ala Val Asn Cys Phe Ala Phe Gly Glu Gly Trp Lys Phe 130 135 140 Ala Phe Ala Lys Asn Ser Asp Leu Pro Thr Ser Thr Phe Lys Lys Tyr 145 150 155 160 Thr Glu Glu Gln Arg Lys Gln Leu Ile Ala Ser Leu Ile Pro Val Thr 165 170 175 Trp Pro Pro Lys Pro Pro Phe Ser Asp Asp Pro Phe His Leu Leu Asp 180 185 190 Glu Met Ile Ala Ala Pro Glu Glu Glu Pro Val Ile Glu Glu Ser Ser 195 200 205 Glu Leu Lys Glu Val Lys Glu Asp Ile Asp Val Val Lys Val Lys Ser 210 215 220 179825DNAStreptococcus faecium 179atgaattact ctatcaatga acaattatta agatttaaat ttttaattga agattcaata 60aaagaaggtg gtacaatagg aaaaacttct atgattagat cttcaaaaat gattaacctc 120attcatgatg ctacaaagca agaattgata tgtaacggtg ttaaccccga taatatccgt 180ccaccgcttg gacattcaaa acctgaatta aaaattgctg gcatacttaa acagaaagac 240caagatgttt gtgttattcc aaccggcata taccccaccc caacccctat tacttgggga 300cctctggctt ttaataaaaa aatcgatcct tacggttttg aatttagtga aaaaacttta 360atcatcaatg ttcgtagcca aatgagtagc ttagccaaaa atgcagacac tttgtttgaa 420agaacctttg cagaagcaca aaatttgcac ttaagatatc ctaatgccgt tttaggagag 480gtatatctaa ttccagttaa tgaatatgat gatgctcttg tatctaaaca tcaagtaggt 540tttaaaactc gtcagactga tttagaaaaa tacattagct tctttactga aatcaataat 600cgttctattg gtgaacctcc acattcttat gagcggtgtg cattattgat cgttgatttt 660aatcaacctc aacctcttct attttcgaat agtgatgaat taaaagctgc tggttacatc 720tcttctgatt ttgatattga atatgcaaat attaattttc aaaattttgc ctcagatatt 780ttaagcatct atgatcagcg ttttgacatc aattatctaa tataa 825180274PRTStreptococcus faecium 180Met Asn Tyr Ser Ile Asn Glu Gln Leu Leu Arg Phe Lys Phe Leu Ile 1 5 10 15 Glu Asp Ser Ile Lys Glu Gly Gly Thr Ile Gly Lys Thr Ser Met Ile 20 25 30 Arg Ser Ser Lys Met Ile Asn Leu Ile His Asp Ala Thr Lys Gln Glu 35 40 45 Leu Ile Cys Asn Gly Val Asn Pro Asp Asn Ile Arg Pro Pro Leu Gly 50 55 60 His Ser Lys Pro Glu Leu Lys Ile Ala Gly Ile Leu Lys Gln Lys Asp 65 70 75 80 Gln Asp Val Cys Val Ile Pro Thr Gly Ile Tyr Pro Thr Pro Thr Pro 85 90 95 Ile Thr Trp Gly Pro Leu Ala Phe Asn Lys Lys Ile Asp Pro Tyr Gly 100 105 110 Phe Glu Phe Ser Glu Lys Thr Leu Ile Ile Asn Val Arg Ser Gln Met 115 120 125 Ser Ser Leu Ala Lys Asn Ala Asp Thr Leu Phe Glu Arg Thr Phe Ala 130 135 140 Glu Ala Gln Asn Leu His Leu Arg Tyr Pro Asn Ala Val Leu Gly Glu 145 150 155 160 Val Tyr Leu Ile Pro Val Asn Glu Tyr Asp Asp Ala Leu Val Ser Lys 165 170 175 His Gln Val Gly Phe Lys Thr Arg Gln Thr Asp Leu Glu Lys Tyr Ile 180 185 190 Ser Phe Phe Thr Glu Ile Asn Asn Arg Ser Ile Gly Glu Pro Pro His 195 200 205 Ser Tyr Glu Arg Cys Ala Leu Leu Ile Val Asp Phe Asn Gln Pro Gln 210 215 220 Pro Leu Leu Phe Ser Asn Ser Asp Glu Leu Lys Ala Ala Gly Tyr Ile 225 230 235 240 Ser Ser Asp Phe Asp Ile Glu Tyr Ala Asn Ile Asn Phe Gln Asn Phe 245 250 255 Ala Ser Asp Ile Leu Ser Ile Tyr Asp Gln Arg Phe Asp Ile Asn Tyr 260 265 270 Leu Ile 181768DNAStreptomyces tubercidicus 181gtgtcagtga gtgcggtcga acaggtattt ttggaatgcg agcgcgctcg ggcagacggt 60gacttgattc agcgggtctc cgccagtgat aaggagtacc actttcagaa ttgggtgcag 120gcccgcatag aggcatgcag gctttcgtac gatgatcctg gccggaacac ctatccggac 180ttccggctca tccatcaccc ggaagggtat gaggtcaagg gcctggagtt tcccggccgc 240gaggcggact acgactcaaa ctcccaggtg cccaccggta accacggcgg ccgtgaggtc 300ttctacgtgt tcggtcgcta cccgaaggca gagcgcggcg tcgatgagta tccagttgta 360gatctggtgg tgtgccacgg cagcttcctc aatgccgata gtgagtacgt tcataagaac 420aagtcgttcc gtggctttgg ctcgtacgga gacatcctgg tccgcgaccg caaaatgtac 480gtcgtgccaa cgccgttcgc actagcttcc ggaaccgcag ggctcgcgac cctgatcgtg 540cctactgaat tcgagccaca gtcggatact ctcgttcagg tgggtgaact tgatcggacc 600gaggttgacg aggtcatcgt gtcgtacgag ttcaaccttc agacaaatga gatggtgacg 660cataaggcgc cgaatctcaa tgcaggtaag gtccacagtt tccgagcata tcgctcgcgc 720ggtgcgggcg attctaagcc ggtttccctc gcagggggtc ggctgtga 768182255PRTStreptomyces tubercidicus 182Met Ser Val Ser Ala Val Glu Gln Val Phe Leu Glu Cys Glu Arg Ala 1 5 10 15 Arg Ala Asp Gly Asp Leu Ile Gln Arg Val Ser Ala Ser Asp Lys Glu 20 25 30 Tyr His Phe Gln Asn Trp Val Gln Ala Arg Ile Glu Ala Cys Arg Leu 35 40 45 Ser Tyr Asp Asp Pro Gly Arg Asn Thr Tyr Pro Asp Phe Arg Leu Ile 50 55 60 His His Pro Glu Gly Tyr Glu Val Lys Gly Leu Glu Phe Pro Gly Arg 65 70 75 80 Glu Ala Asp Tyr Asp Ser Asn Ser Gln Val Pro Thr Gly Asn His Gly 85 90 95 Gly Arg Glu Val Phe Tyr Val Phe Gly Arg Tyr Pro Lys Ala Glu Arg 100 105 110 Gly Val Asp Glu Tyr Pro Val Val Asp Leu Val Val Cys His Gly Ser 115 120 125 Phe Leu Asn Ala Asp Ser Glu Tyr Val His Lys Asn Lys Ser Phe Arg 130 135 140 Gly Phe Gly Ser Tyr Gly Asp Ile Leu Val Arg Asp Arg Lys Met Tyr 145 150 155 160 Val Val Pro Thr Pro Phe Ala Leu Ala Ser Gly Thr Ala Gly Leu Ala 165 170 175 Thr Leu Ile Val Pro Thr Glu Phe Glu Pro Gln Ser Asp Thr Leu Val 180 185 190 Gln Val Gly Glu Leu Asp Arg Thr Glu Val Asp Glu Val Ile Val Ser 195 200 205 Tyr Glu Phe Asn Leu Gln Thr Asn Glu Met Val Thr His Lys Ala Pro 210 215 220 Asn Leu Asn Ala Gly Lys Val His Ser Phe Arg Ala Tyr Arg Ser Arg 225 230 235 240 Gly Ala Gly Asp Ser Lys Pro Val Ser Leu Ala Gly Gly Arg Leu 245 250 255 1831161DNASalmonella typhi 27 183ttgtttttaa ctgtcatttt tcctaactat cgatctgtta ccatacaacc tgctatctta 60gcatctcatt tttactatgt ccatggtgag gatatgaatt tcaaggataa aaattgtttc 120cctaacgaac tcatagcgtt ggcgaaaatt tcaaaaaatg atgttttaga taagttcgga 180acggatgttt ttaaaaaggt tgtttatgat gttttaacag gtaaaaatgt tcgcgaattc 240actgaaatac taactcgtac tagattgtta gaaagcaatc tctctttttt tgactttttt 300gtggataaaa tgaaagaggg gataacgcca aagcagcttt atctctatgc aaaaaatgca 360ttatcgaaca agtcttatgt taagtataat caacctgttc tcgagtggat ggttatgatg 420acaaataaac agacccaaaa tgttttaaga gatgagcatg gggatggttt tgataggctt 480gctttaagga cgcaagaaga aatacttaaa ataaaaaacg ggtatgaaga taaaattgga 540gagatatcta ttggtgggca aaaggtgtct ttagaagatt tttgctatat tattttatct 600cttggttcgc aaactttaac tattagggga tctgagaaat ctcttcatgg taaatatttt 660gaaaagctaa tactcggttc tttatttaca ataatgggtt ttgaatataa agaaaaaatt 720gaagaagggt taaatgctaa atgttttact ctttcaacaa gagctgatga cagggagtct 780gatgctactc ttatttttaa tgggaaggcg attagggttg atattggttt tattggtagg 840ggtaacacag aaataagttt ggataaagta tctagattta gacgaatgga tgatattggc 900ggagtgatgc ataatataag cacaatggtt attgttgacg ttattggtga tagaagtaga 960atagttaata tggctgaaga gattgatggt aaagttgttg cgatgagtga cccgtattgg 1020gttgcaaagg tctcttccta tattagttcg aaactgaatg tagatgatct tttagaggat 1080aaacctcaac ttaaatacat acagtctttt atatctgatg cattagagaa tgtagatctg 1140gaaaaataca ttaaattata a 1161184386PRTSalmonella typhi 27 184Met Phe Leu Thr Val Ile Phe Pro Asn Tyr Arg Ser Val Thr Ile Gln 1 5 10 15 Pro Ala Ile Leu Ala Ser His Phe Tyr Tyr Val His Gly Glu Asp Met 20 25 30 Asn Phe Lys Asp Lys Asn Cys Phe Pro Asn Glu Leu Ile Ala Leu Ala 35 40 45 Lys Ile Ser Lys Asn Asp Val Leu Asp Lys Phe Gly Thr Asp Val Phe 50 55 60 Lys Lys Val Val Tyr Asp Val Leu Thr Gly Lys Asn Val Arg Glu Phe 65 70 75 80 Thr Glu Ile Leu Thr Arg Thr Arg Leu Leu Glu Ser Asn Leu Ser Phe 85 90 95 Phe Asp Phe Phe Val Asp Lys Met Lys Glu Gly Ile Thr Pro Lys Gln 100 105 110 Leu Tyr Leu Tyr Ala Lys Asn Ala Leu Ser Asn Lys Ser Tyr Val Lys 115 120 125 Tyr Asn Gln Pro Val Leu Glu Trp Met Val Met Met Thr Asn Lys Gln 130 135 140 Thr Gln Asn Val Leu Arg Asp Glu His Gly Asp Gly Phe Asp Arg Leu 145 150 155 160 Ala Leu Arg Thr Gln Glu Glu Ile Leu Lys Ile Lys Asn Gly Tyr Glu 165 170 175 Asp Lys Ile Gly Glu Ile Ser Ile Gly Gly Gln Lys Val Ser Leu Glu 180 185 190 Asp Phe Cys Tyr Ile Ile Leu Ser Leu Gly Ser Gln Thr Leu Thr Ile 195 200 205 Arg Gly Ser Glu Lys Ser Leu His Gly Lys Tyr Phe Glu Lys Leu Ile 210 215 220 Leu Gly Ser Leu Phe Thr Ile Met Gly Phe Glu Tyr Lys Glu Lys Ile 225 230 235 240 Glu Glu Gly Leu Asn Ala Lys Cys Phe Thr Leu Ser Thr Arg Ala Asp 245 250 255 Asp Arg Glu Ser Asp Ala Thr Leu Ile Phe Asn Gly Lys Ala Ile Arg 260 265 270 Val Asp Ile Gly Phe Ile Gly Arg Gly Asn Thr Glu Ile Ser Leu Asp 275 280 285 Lys Val Ser Arg Phe Arg Arg Met Asp Asp Ile Gly Gly Val Met His 290 295 300 Asn Ile Ser Thr Met Val Ile Val Asp Val Ile Gly Asp Arg Ser Arg 305 310 315 320 Ile Val Asn Met Ala Glu Glu Ile Asp Gly Lys Val Val Ala Met Ser 325 330 335 Asp Pro Tyr Trp Val Ala Lys Val Ser Ser Tyr Ile Ser Ser Lys Leu 340 345 350 Asn Val Asp Asp Leu Leu Glu Asp Lys Pro Gln Leu Lys Tyr Ile Gln 355 360 365 Ser Phe Ile Ser Asp Ala Leu Glu Asn Val Asp Leu Glu Lys Tyr Ile 370 375 380 Lys Leu 385 185858DNABacillus species 185atgacaaaag ttaaagaatt gtttggattg aatacaagtg ttaaagggac tgattgggga 60aaagttgtta cagaacagca ttgccccttt ttgaataaaa agtgtataaa aaatagaaag 120agtcaggcag aaatagcaat tggcacatgt actatgagtt atggcaaagt aagtaaagat 180ataattatct gtccacatag attactagaa aacagaaaaa tattcataga ttgtattcat 240ttactaacaa tgcatgagcc tggtaatgag ttacatgttg tatcagaagt gtctattcca 300ggaggtaatg tagattactt tttagtctca gcaaaagatg gtaaagtaaa agattttgtt 360gggattgagc tacagactat ggataccaca ggtactgtat ggcctgaaag agaaaggttt 420ctaaaagatg ctggatatag tggatatgat aaagaggcaa tagactctga taagtccttt

480ggaatgaatt ggaagcatac agcaaaaact attcttgtac agttacatca taaggtcaaa 540acctttgagc atgttaataa aaaattggtc ttagtgatac aagaaccttt aatagattat 600atgaaaaaag aatttagttt ttctcatgta ggaaatgcta agttaggtga cccactacat 660tttcacccat attccttaga tactagagaa gataatcaat tacatttaaa tttaaaaact 720agacttagta cagactcaga tggaatggca atgtgtttag gattacaagc tgaggcaaaa 780gttgaattaa cagaaattat tgctaaatta gaagaaaaaa tgaaaaatgc tacagtcagt 840acattattaa ctttgtaa 858186285PRTBacillus species 186Met Thr Lys Val Lys Glu Leu Phe Gly Leu Asn Thr Ser Val Lys Gly 1 5 10 15 Thr Asp Trp Gly Lys Val Val Thr Glu Gln His Cys Pro Phe Leu Asn 20 25 30 Lys Lys Cys Ile Lys Asn Arg Lys Ser Gln Ala Glu Ile Ala Ile Gly 35 40 45 Thr Cys Thr Met Ser Tyr Gly Lys Val Ser Lys Asp Ile Ile Ile Cys 50 55 60 Pro His Arg Leu Leu Glu Asn Arg Lys Ile Phe Ile Asp Cys Ile His 65 70 75 80 Leu Leu Thr Met His Glu Pro Gly Asn Glu Leu His Val Val Ser Glu 85 90 95 Val Ser Ile Pro Gly Gly Asn Val Asp Tyr Phe Leu Val Ser Ala Lys 100 105 110 Asp Gly Lys Val Lys Asp Phe Val Gly Ile Glu Leu Gln Thr Met Asp 115 120 125 Thr Thr Gly Thr Val Trp Pro Glu Arg Glu Arg Phe Leu Lys Asp Ala 130 135 140 Gly Tyr Ser Gly Tyr Asp Lys Glu Ala Ile Asp Ser Asp Lys Ser Phe 145 150 155 160 Gly Met Asn Trp Lys His Thr Ala Lys Thr Ile Leu Val Gln Leu His 165 170 175 His Lys Val Lys Thr Phe Glu His Val Asn Lys Lys Leu Val Leu Val 180 185 190 Ile Gln Glu Pro Leu Ile Asp Tyr Met Lys Lys Glu Phe Ser Phe Ser 195 200 205 His Val Gly Asn Ala Lys Leu Gly Asp Pro Leu His Phe His Pro Tyr 210 215 220 Ser Leu Asp Thr Arg Glu Asp Asn Gln Leu His Leu Asn Leu Lys Thr 225 230 235 240 Arg Leu Ser Thr Asp Ser Asp Gly Met Ala Met Cys Leu Gly Leu Gln 245 250 255 Ala Glu Ala Lys Val Glu Leu Thr Glu Ile Ile Ala Lys Leu Glu Glu 260 265 270 Lys Met Lys Asn Ala Thr Val Ser Thr Leu Leu Thr Leu 275 280 285 1871292DNABacillus sphaericus 187atgagacgat tagcaaaaaa ttcacggaac gacagttatt taagtaatag ggattaccag 60gaaatcgtga gggaaaatac cactacaata tcgtttccct taaaagaaaa acatactctg 120actttaacga aaaaaatagg gctaaatcag actgctggat tcggaggatg gtttttccct 180gattcaccat gtttattaac agtaactgta ctatcctctt tcggtacaaa ggtaacttct 240aaaaccttta gcctttctaa agattggaat cgtgttgggc ttgcttggat taacgagcat 300tcgagtgaca ccatagcatt gtcctagagt ttagtgatgt ggaaatagtt catacatggg 360gacttacatg tgatgttttt aatgtccatg aattaattat tgatgctata gaagatcaaa 420ataaactaat agacgtgcta aatcaagaac atttatctcc tgaaacatat tatttaaacc 480atgactctga tactgattta attgagaatt tggaatctac agaagagata aagatagtta 540accaaagcca aaagcaaatc tctttaaaaa aatgctgtta ttgtcaacgt tatatgcctg 600tgaacatatt agttcgttca aattcatcat ttcataaaca caagagtaag aaaactggtt 660ttcaaaatga atgtcgggct tgtaagaagt ggagaataaa taattcattc aatccagtca 720gaacaaaaga ccaactacat gaatcagcag ttattacacg tgaaaaaaaa atattactta 780aagaacctga aatattacag aaaatcaaaa atagaaataa cggtgagggc ttaaaaagta 840ttatatggaa aaaatttgat aaaaaatgct ttaattgtga aaaagaatta accattgaag 900aggtacgcct agaccataca agaccacttg cttatctgtg gcctatcgat gaacacgcaa 960cttgtttatg tgaaaaatgc aacaatacaa aacatgatat gtttcctatc gatttttatc 1020aaggggacga agacaaatta agacgtttag ctagaattac ggggttagat tatgaatctc 1080tagttaagag ggacgtaaat gaagttgaac ttgcaagaat aatcaataac attgaagact 1140ttgcaactaa tgtagaggca cgtacttttc gctcaataag aaataaagta aaagaagtac 1200gtcccgatac tgacctattt gaaattctta aatctaaaaa tattaattta tataatgaac 1260ttcaatatga acttcttacc cgtaaggatt aa 1292188430PRTBacillus sphaericus 188Met Arg Arg Leu Ala Lys Asn Ser Arg Asn Asp Ser Tyr Leu Ser Asn 1 5 10 15 Arg Asp Tyr Gln Glu Ile Val Arg Glu Asn Thr Thr Thr Ile Ser Phe 20 25 30 Pro Leu Lys Glu Lys His Thr Leu Thr Leu Thr Lys Lys Ile Gly Leu 35 40 45 Asn Gln Thr Ala Gly Phe Gly Gly Trp Phe Phe Pro Asp Ser Pro Cys 50 55 60 Leu Leu Thr Val Thr Val Leu Ser Ser Phe Gly Thr Lys Val Thr Ser 65 70 75 80 Lys Thr Phe Ser Leu Ser Lys Asp Trp Asn Arg Val Gly Leu Ala Trp 85 90 95 Ile Asn Glu His Ser Ser Asp Thr Met Ser Ile Val Leu Glu Phe Ser 100 105 110 Asp Val Glu Ile Val His Thr Trp Gly Leu Thr Cys Asp Val Phe Asn 115 120 125 Val His Glu Leu Ile Ile Asp Ala Ile Glu Asp Gln Asn Lys Leu Ile 130 135 140 Asp Val Leu Asn Gln Glu His Leu Ser Pro Glu Thr Tyr Tyr Leu Asn 145 150 155 160 His Asp Ser Asp Thr Asp Leu Ile Glu Asn Leu Glu Ser Thr Glu Glu 165 170 175 Ile Lys Ile Val Asn Gln Ser Gln Lys Gln Ile Ser Leu Lys Lys Cys 180 185 190 Cys Tyr Cys Gln Arg Tyr Met Pro Val Asn Ile Leu Val Arg Ser Asn 195 200 205 Ser Ser Phe His Lys His Lys Ser Lys Lys Thr Gly Phe Gln Asn Glu 210 215 220 Cys Arg Ala Cys Lys Lys Trp Arg Ile Asn Asn Ser Phe Asn Pro Val 225 230 235 240 Arg Thr Lys Asp Gln Leu His Glu Ser Ala Val Ile Thr Arg Glu Lys 245 250 255 Lys Ile Leu Leu Lys Glu Pro Glu Ile Leu Gln Lys Ile Lys Asn Arg 260 265 270 Asn Asn Gly Glu Gly Leu Lys Ser Ile Ile Trp Lys Lys Phe Asp Lys 275 280 285 Lys Cys Phe Asn Cys Glu Lys Glu Leu Thr Ile Glu Glu Val Arg Leu 290 295 300 Asp His Thr Arg Pro Leu Ala Tyr Leu Trp Pro Ile Asp Glu His Ala 305 310 315 320 Thr Cys Leu Cys Glu Lys Cys Asn Asn Thr Lys His Asp Met Phe Pro 325 330 335 Ile Asp Phe Tyr Gln Gly Asp Glu Asp Lys Leu Arg Arg Leu Ala Arg 340 345 350 Ile Thr Gly Leu Asp Tyr Glu Ser Leu Val Lys Arg Asp Val Asn Glu 355 360 365 Val Glu Leu Ala Arg Ile Ile Asn Asn Ile Glu Asp Phe Ala Thr Asn 370 375 380 Val Glu Ala Arg Thr Phe Arg Ser Ile Arg Asn Lys Val Lys Glu Val 385 390 395 400 Arg Pro Asp Thr Asp Leu Phe Glu Ile Leu Lys Ser Lys Asn Ile Asn 405 410 415 Leu Tyr Asn Glu Leu Gln Tyr Glu Leu Leu Thr Arg Lys Asp 420 425 430 189278PRTunknownBacillus caldolyticus 189Met Gln Pro Asn Pro Lys Phe Ile Asn Lys Ser Ser Ala Phe Trp Ala 1 5 10 15 Tyr Ala Lys Leu Leu Ser Glu Gln Leu Gly Tyr Ser Lys Asp Gly Val 20 25 30 Val Ile Ser Tyr Ser Glu Ala Gln Ala Arg Ala Lys Leu Lys Lys Leu 35 40 45 Gly Ile Asn Val Lys Glu Gly Ile Phe Lys Asp Val Leu Arg Tyr Leu 50 55 60 Lys Tyr Arg Ala Glu Leu Leu Asn Lys His Lys Asp Tyr Leu Met Asp 65 70 75 80 Val Glu Glu Ala Arg Lys Tyr Phe Gln Val Ala Leu Lys Gln His Gln 85 90 95 Gln Asn Asn Tyr Thr Cys Lys Leu Pro Leu Asn Lys Gln Lys Asn Glu 100 105 110 Lys Lys Asp Tyr Ala Tyr Phe Thr Cys Ile Ile Asn Ile Ile Ala Glu 115 120 125 Thr Glu Leu Arg Tyr Phe Ala Asn Asn Asn Gly Leu Val Tyr Gly Lys 130 135 140 Asp Ile Tyr Phe Asp Asp Asn Pro Met Asn Leu Ser Tyr Ile Leu Asn 145 150 155 160 Phe Asn Arg Glu Leu Glu Gly Ile Met Ser Arg Arg Phe Asp Gly Ala 165 170 175 Phe Pro Ser Thr Val Asn Pro Ile Leu Ile Trp Glu Ile Lys Glu Tyr 180 185 190 Tyr Tyr Thr Thr Thr Phe Gly Ser Arg Ile Ala Asp Gly Val Tyr Glu 195 200 205 Thr Gln Leu Asp Gly Tyr Glu Ile Lys Thr Ile Arg Glu Glu Thr Asn 210 215 220 Lys Asn Ile Gln His Ile Tyr Phe Ile Asp Asp Tyr Asn Thr Trp Trp 225 230 235 240 Asn Met Gly Lys Ser Tyr Leu Cys Arg Ile Ile Asp Met Leu His Met 245 250 255 Gly Leu Val Asp Glu Val Ile Met Gly Lys Glu Val Phe Glu Arg Trp 260 265 270 Pro Gln Ile Leu Arg Ala 275 190293PRTunknownCorynebacterium glutamicum 190Met Lys Ala Glu Pro Lys Trp Ile Asn Arg Pro Pro Gln Phe Trp His 1 5 10 15 Tyr Val Arg Val Ile Ser Gln His Leu Gly Tyr Ala Arg Lys Gly Glu 20 25 30 Ile Tyr Arg His Glu Pro Glu Ala Ile Glu Arg Ala Leu Arg Glu Leu 35 40 45 Glu Leu Ser Val Asp Ala Leu Arg Leu Thr Pro Ile Pro Gly Leu Ser 50 55 60 Val Gly Glu Leu Ala Glu Tyr Phe Asp Phe Arg Ala Asp Leu Ile His 65 70 75 80 Gly Thr Ile Ala Ala Asn Leu Gln Asn Ala Ser Glu Ala Lys Lys Thr 85 90 95 Phe Glu Gln Val Val Glu Lys Phe Thr Thr Gly Met Thr Pro Gln Phe 100 105 110 Lys Gly Gly Lys Glu Asn Ser Arg Leu Tyr Arg Val Asn Gly Gly Val 115 120 125 Pro Val Val Val Pro Tyr Asn Lys Gln Lys Gly Asp Lys Arg Asp Ile 130 135 140 Asp Phe Leu Thr Gly Thr Thr Asn Ile Leu Leu Ser Tyr Tyr Leu Gly 145 150 155 160 Gly Glu Ser Phe Asp Gln Asp Pro Arg Gln Leu Pro Val Val Thr Glu 165 170 175 Asp Gly Val Val Ser Gly Ser Met Ser Arg Arg Met Asp Gly Ala Tyr 180 185 190 Pro Asp Ser Val Asn Pro Ser Ala Ile Trp Glu Phe Lys Cys Tyr Tyr 195 200 205 Tyr Thr Thr Thr Phe Gly Ser Lys Ile Ser Asp Ala Val Tyr Ile Thr 210 215 220 Asp Leu Asp Gly Tyr Glu Arg Gly Glu Ile Leu Lys Ala Ser His Lys 225 230 235 240 Arg Val Glu Asn Asn Val Phe Leu Asp Ala Tyr Ser Val Phe Met Glu 245 250 255 Gln Gly Leu Ser Phe Leu Val Arg Leu Val Asp Met Leu Gln Arg Gly 260 265 270 Ala Val Asp Asn Leu Val Phe Gly Lys Glu Val Leu Thr Ala Val Pro 275 280 285 Glu Ile Val Lys Gly 290 191137PRTunknownBacillus caldolyticus 191Lys Asp Ile Tyr Phe Asp Asp Asn Pro Met Asn Leu Ser Tyr Ile Leu 1 5 10 15 Asn Phe Asn Arg Glu Leu Glu Gly Ile Met Ser Arg Arg Phe Asp Gly 20 25 30 Ala Phe Pro Ser Thr Val Asn Pro Ile Leu Ile Trp Glu Ile Lys Glu 35 40 45 Tyr Tyr Tyr Thr Thr Thr Phe Gly Ser Arg Ile Ala Asp Gly Val Tyr 50 55 60 Glu Thr Gln Leu Asp Gly Tyr Glu Ile Lys Thr Ile Arg Glu Glu Thr 65 70 75 80 Asn Lys Asn Ile Gln His Ile Tyr Phe Ile Asp Asp Tyr Asn Thr Trp 85 90 95 Trp Asn Met Gly Lys Ser Tyr Leu Cys Arg Ile Ile Asp Met Leu His 100 105 110 Met Gly Leu Val Asp Glu Val Ile Met Gly Lys Glu Val Phe Glu Arg 115 120 125 Trp Pro Gln Ile Leu Arg Ala Val Leu 130 135 192194PRTunknownMagnetococcus sp. MC-1 192Lys Gly Ile Gln Phe Asp Pro Asp Pro Gln Asn Arg Cys Val Trp Ile 1 5 10 15 Asn Asp Asn Arg Leu His Val Thr Ser Arg Asn Leu Asp Gly Ala Ile 20 25 30 Pro Gly Leu Thr Asn Pro Glu Ile Ile Trp Glu Ile Lys Glu Tyr Trp 35 40 45 Gly Lys Thr Lys Gly Gly Ser Lys Met Ser Asp Gly Val Tyr Glu Thr 50 55 60 Gln Leu Asp Gly Tyr Glu Ile Lys Thr Ile Arg Glu Glu Thr Asn Lys 65 70 75 80 Asn Ile Gln His Ile Tyr Phe Ile Asp Asp Tyr Asn Thr Trp Trp Asn 85 90 95 Met Gly Lys Ser Tyr Leu Cys Arg Ile Ile Asp Met Leu His Met Gly 100 105 110 Leu Val Asp Glu Val Ile Asp Ala Val Tyr Glu Cys Gln Leu Val Gly 115 120 125 Arg Glu Leu Arg Glu Tyr Glu Glu Lys Cys Asn Lys Lys Ile Met His 130 135 140 Phe Val Phe Leu Asp Gly Lys Asp Gln Trp Ser His Arg Lys Ser Asp 145 150 155 160 Leu Lys Arg Phe Ile Asp Leu Trp Cys Gln Gly Leu Ile Asp Thr Leu 165 170 175 Phe Val Gly Lys Gln Val Glu Ser Leu Trp Glu Lys Thr Leu Glu Lys 180 185 190 Leu Leu 193297PRTunknownEnterobacter agglomerans 193Lys Lys Arg Arg Asp Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp 1 5 10 15 Leu Ser Pro Glu Val Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe 20 25 30 Leu Asn Lys Glu Cys Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr 35 40 45 Gly Thr Cys Ser Val Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro 50 55 60 Asn Arg Leu Tyr Ala Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg 65 70 75 80 Asp Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile 85 90 95 Lys Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn 100 105 110 Ser Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val 115 120 125 Leu Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu 130 135 140 Ile Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala 145 150 155 160 Tyr Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser 165 170 175 Gln His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln 180 185 190 Ile Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys 195 200 205 Gly Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp 210 215 220 Val Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser 225 230 235 240 Ile Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu 245 250 255 Gln Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu 260 265 270 Phe Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp 275 280 285 Leu Asp Ala Val Ile Lys Lys Ala Leu 290 295 194276PRTunknownChloroflexus aurantiacus J-10-fl 194Gln Thr Gln Gln Pro Leu Ala Glu Val Phe Gly Tyr Gln Ile Thr Asp 1 5 10 15 Gln Ser Glu Ala Ala Ala Arg Cys Arg Ser Ala Arg Leu Cys Pro Phe 20 25 30 Gln Gly Gln Asp Arg Lys Cys Thr Lys Asp Lys Ala Asn Asn Pro Leu 35 40 45 Gly Val Cys Ala Ile Tyr His Asn Asn Glu Pro Val Ile Thr Cys Pro 50 55 60 Ile Arg Phe Arg Gln Asn Trp Leu Ile Ala Gln Asp Ala Ala Leu Phe 65 70

75 80 Phe Phe Gly Glu Gly Thr Arg Trp Ser Ile Leu Thr Glu Ile Arg Leu 85 90 95 Pro Asp Ala Phe Gly Lys Ser Ala Gly Asn Ile Asp Val Val Leu Val 100 105 110 Ser Tyr Asp Asp Glu Gly Arg Ile Thr Asp Phe Gly Ala Ile Glu Ile 115 120 125 Gln Ala Val Tyr Ile Ser Gly Asn Val Arg Ser Phe Phe Glu His Tyr 130 135 140 Met Arg Asp Pro Gln Gly Tyr Ile Val Gly Asp Trp Ile Gly Glu Thr 145 150 155 160 Pro Val Pro Arg Pro Asp Tyr Leu Ser Ser Ser Arg Lys Arg Leu Val 165 170 175 Pro Gln Leu Met Tyr Lys Gly Ala Ile Leu Arg Ala Trp Asn Lys Lys 180 185 190 Met Ala Val Val Val Asp Glu Gln Phe Phe Gln Thr Leu Pro Gln Leu 195 200 205 Ala Ser Ile Pro Pro Gln Asp Ala Asn Met Ala Trp Phe Ile Tyr Arg 210 215 220 Leu Met Pro Gly Arg Gln Ala His Glu Gly Thr Glu Arg Tyr Tyr Leu 225 230 235 240 Glu Lys Val Thr Glu Val Phe Thr Asp Phe Glu Gln Val Ile Arg Val 245 250 255 Met Thr Thr Ser Ser Pro Gly Arg Ser Glu Asp Phe Ile Lys Phe Leu 260 265 270 Gln Ala Lys Leu 275 195294PRTunknownEnterobacter agglomerans 195Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr Ala 50 55 60 Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly Asp 65 70 75 80 Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala Thr 85 90 95 Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu Val 100 105 110 Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile Thr 115 120 125 Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile Asp 130 135 140 Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu Lys 145 150 155 160 Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu Asn 165 170 175 Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys Gly 180 185 190 Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe Ile 195 200 205 Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala Asp 210 215 220 Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His Thr 225 230 235 240 Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln Arg Lys Leu Ile 245 250 255 Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe Ser Lys Arg Phe 260 265 270 Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp Leu Asp Ala Val Ile 275 280 285 Lys Lys Ala Leu Gly Met 290 196297PRTunknownRhodobacterales bacterium HTCC2654 196Ile Phe Glu Phe Phe Gly Tyr Arg Ala Asp Asp Arg Ser Asp Ile Ala 1 5 10 15 Lys His Ala Ala Asp Thr Glu Val Cys Pro Ile Ser Gly Glu Thr Cys 20 25 30 Gln Lys Ser Phe Asn Asp Gly Val Val Ser Gly Val Cys Ala Ile Lys 35 40 45 Pro Ile Thr Ser Glu Pro Val Ile Cys Cys Pro Ile Arg Leu Tyr Ala 50 55 60 Asp Asp Tyr Arg Ile Leu Ser Asp Ile Ala Asp Arg Val Phe Gly Pro 65 70 75 80 Asn Leu Lys Leu Val Ala Gly Arg Asp Ala Val Asn Tyr Ser Ile Asp 85 90 95 Asn Arg Glu Ala Cys Val Ala Val Phe Gly Lys Gly Trp Gly Gly Glu 100 105 110 Leu Arg Leu Pro Gln Lys Ser Lys Lys Gly Gly Tyr Phe Val Asp Trp 115 120 125 Val Leu Ala Lys Ile Ser Glu Glu Gly Asp Leu Val Glu Phe Val Ala 130 135 140 Val Glu Val Gln Thr Ile Asp Thr Thr Gly Thr Tyr Arg Pro Gly Tyr 145 150 155 160 Asp Ala Leu Lys Gln Asp Gly Leu Val Glu Lys Thr Thr Ala Gly Leu 165 170 175 Asn Trp Glu Asn Val Ala Lys Arg Ile Leu Pro Gln Leu Ile Tyr Lys 180 185 190 Gly Gln Ile Leu Gln Arg Glu Glu Leu Cys Lys Asn Gly Leu Phe Phe 195 200 205 Val Cys Pro Glu Pro Val Phe Arg Arg Ile Met Glu Arg Leu Gly Gly 210 215 220 Gln Glu Gly Leu Val Arg Tyr Ala Leu Gln Pro Ala Ser Ile Thr Phe 225 230 235 240 Ala Val Tyr Asp Tyr Asp Phe Ser Ser Glu Pro Ser Asp Glu Thr Leu 245 250 255 Val Pro Leu Lys Asn Thr Leu Asn His Ser Thr Thr Val Tyr Lys Val 260 265 270 Gln Glu Ala Phe Asn Asn Val Thr Leu Pro Ile Glu Asn Val Tyr Arg 275 280 285 Asp Ala Ile Arg Arg Ala Leu Gly Ile 290 295 197297PRTunknownEnterobacter agglomerans 197Lys Lys Arg Arg Asp Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp 1 5 10 15 Leu Ser Pro Glu Val Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe 20 25 30 Leu Asn Lys Glu Cys Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr 35 40 45 Gly Thr Cys Ser Val Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro 50 55 60 Asn Arg Leu Tyr Ala Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg 65 70 75 80 Asp Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile 85 90 95 Lys Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn 100 105 110 Ser Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val 115 120 125 Leu Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu 130 135 140 Ile Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala 145 150 155 160 Tyr Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser 165 170 175 Gln His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln 180 185 190 Ile Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys 195 200 205 Gly Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp 210 215 220 Val Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser 225 230 235 240 Ile Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu 245 250 255 Gln Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu 260 265 270 Phe Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp 275 280 285 Leu Asp Ala Val Ile Lys Lys Ala Leu 290 295 198333PRTunknownChlorobium phaeobacteroides BS1 198Lys Asn Ala Gln Pro Leu Ala Glu Val Phe Gly His Pro Val Thr Asp 1 5 10 15 Ala Ser Ser Arg Ala Asp Arg Tyr Arg Ser Gln Arg Leu Cys Pro Phe 20 25 30 Asn Asn Lys Val Pro Asn Cys Thr Lys Asp Lys Ala Lys Ser Pro Leu 35 40 45 Gly Val Cys Ser Ile Gln His Asp Gly Ser Pro Val Ile Thr Cys Pro 50 55 60 Ile Arg Phe Arg Glu Asp Trp Leu Ile Thr Asp Asp Ala Ala Ser Phe 65 70 75 80 Phe Phe Pro Glu Gly Thr Lys Trp Ser Ser Leu Thr Glu Ile Arg Leu 85 90 95 Asn Asp Gly Asn Gly Lys Ser Ala Gly Asn Ile Asp Ile Val Leu Val 100 105 110 Ala Tyr Asp Asp Asn Gly Lys Val Lys Asp Phe Gly Ala Leu Glu Ile 115 120 125 Gln Ala Val Tyr Ile Ser Gly Asn Val Arg Asp Pro Phe Glu Tyr Phe 130 135 140 Met Glu Glu Pro Lys Gly Arg Ala Phe Met Asp Trp Ser Asn Gln Pro 145 150 155 160 Asn Tyr Pro Arg Pro Asp Tyr Leu Ser Ser Ser Arg Lys Arg Leu Val 165 170 175 Pro Gln Leu Phe Phe Lys Gly Gly Ile Leu His Ser Trp Lys Lys Lys 180 185 190 Ser Ala Val Ala Leu Asn Lys Ser Phe Phe Asp Thr Leu Pro Pro Leu 195 200 205 Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile 210 215 220 Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln 225 230 235 240 Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe 245 250 255 Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Thr Thr Val Ser Arg 260 265 270 Lys Lys Ala Asp Ile Ala Trp Leu Ile Tyr Asp Ile Glu Leu Cys Gly 275 280 285 Ser Gly Ala Glu Lys Arg Tyr Arg Leu Lys Lys Val Asp Glu Val Phe 290 295 300 Thr Glu Phe Glu Pro Ala Leu Leu Ser Ile Thr Thr Pro Val Pro Gly 305 310 315 320 Arg Ile Asp Asp Phe Met Asn Met Leu Gln Val Lys Ile 325 330 199285PRTunknownEnterobacter agglomerans 199Asp Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu 1 5 10 15 Val Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu 20 25 30 Cys Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser 35 40 45 Val Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr 50 55 60 Ala Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly 65 70 75 80 Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala 85 90 95 Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu 100 105 110 Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile 115 120 125 Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile 130 135 140 Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu 145 150 155 160 Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu 165 170 175 Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys 180 185 190 Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe 195 200 205 Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala 210 215 220 Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His 225 230 235 240 Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln Arg Lys Leu 245 250 255 Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe Ser Lys Arg 260 265 270 Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp Leu 275 280 285 200263PRTunknownChlorobium chlorochromatii CaD3 200Pro Leu Gly Glu Val Phe Gly Phe Ala Ala Thr Asp Gln Ser Pro Lys 1 5 10 15 Ala Gln Arg Tyr Arg Ser His Arg His Cys Pro Phe Asn Asn Lys Ser 20 25 30 Pro Asn Cys Thr Asn Ser His Thr Glu Asn Pro Leu Gly Val Cys Ser 35 40 45 Ile Leu His Asn Asn Lys Ala Ile Ile Thr Cys Pro Ile Arg Phe Arg 50 55 60 Glu Asp Trp Leu Ile Thr Asp Asp Ala Ala Ser Phe Phe Phe Glu Pro 65 70 75 80 Gly Val Arg Trp Ser Ser Leu Thr Asp Val Arg Leu Ala Asp Ala Asn 85 90 95 Gly Thr Ser Ala Gly Asn Met Asp Val Met Leu Val Ala Tyr Asp Lys 100 105 110 Glu Gly Lys Ile Ile Asp Phe Gly Ala Ile Gln Ile Gln Thr Ala His 115 120 125 Ile Asp Gly Asn Val Arg Glu Pro Phe Glu Cys Tyr Met Lys Asp Pro 130 135 140 Lys Thr Asn Ala Met Met Asp Trp Thr Arg Gln Pro Asn Tyr Pro Glu 145 150 155 160 Pro Asp Phe Leu Ser Ala Met Arg Thr Ser Val Val Pro Glu Leu Leu 165 170 175 Tyr Lys Gly Gly Ile Leu His Ser Trp Asn Lys Lys Met Ala Ile Ala 180 185 190 Ile Asn Lys Ser Met Phe Glu Thr Leu Pro Pro Leu Thr Arg Val Lys 195 200 205 Lys Asp Glu Ala Asp Ile Ala Trp Leu Leu Tyr Glu Leu Glu Ala Val 210 215 220 Asn Asp Gly Glu Lys Glu Ala Tyr Gln Leu Lys Lys Ser Glu Val Val 225 230 235 240 Tyr Thr Ala Phe Gln Pro Thr Leu Leu Ala Leu Thr Ala Ile Ala Pro 245 250 255 Gly Asn Val Asn Asp Phe Met 260 201280PRTunknownEnterobacter agglomerans 201Leu Ser Pro Glu Val Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe 1 5 10 15 Leu Asn Lys Glu Cys Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr 20 25 30 Gly Thr Cys Ser Val Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro 35 40 45 Asn Arg Leu Tyr Ala Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg 50 55 60 Asp Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile 65 70 75 80 Lys Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn 85 90 95 Ser Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val 100 105 110 Leu Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu 115 120 125 Ile Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala 130 135 140 Tyr Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser 145 150 155 160 Gln His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln 165 170 175 Ile Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys 180 185 190 Gly Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp 195 200 205 Val Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser 210 215 220 Ile Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu 225 230 235 240 Gln Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu 245 250 255 Phe Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp 260 265 270 Leu Asp Ala Val Ile Lys Lys Ala 275 280 202271PRTJannaschia sp. CCS1 202Arg Ser

Lys Leu Ala Gln Gln Gln Ala Glu Glu Leu Pro Cys Pro Phe 1 5 10 15 Arg Thr Asp Ser Pro His Pro Thr Cys Thr Lys Pro Gly Gly Val Cys 20 25 30 Ser Ile Arg Ile Tyr Arg Glu Glu Ala Gly Val Ile Ala Pro Ile Asp 35 40 45 Gly Glu Arg Gly Arg Leu Arg Ala Leu Cys Pro Trp Arg Phe His Gln 50 55 60 Asp Gly Thr Ala Phe Asp Lys Ile Gly Glu Ser Leu Leu Ala Asp Pro 65 70 75 80 Ser Pro Leu Arg Ala Gly Glu Val Gly Phe Leu Glu Ser Thr Gly Asn 85 90 95 Leu Asp Ser Ala Ala Gly Glu Asp Val Gly Arg Ile Asp Met Ile Leu 100 105 110 Val Lys Ser Asn Ser Val Asp Gly Ala Pro Met Asp Trp Val Ala Val 115 120 125 Glu Val Gln Ala Val Tyr Phe Ser Gly Lys Lys Met Ser Ile Glu Phe 130 135 140 Asp His Leu Lys Leu Thr Gln Gly Arg Leu Ser Met Ala Gln Glu Lys 145 150 155 160 Arg Arg Pro Asp Tyr Arg Ser Ser Gly Val Lys Arg Leu Met Pro Gln 165 170 175 Leu Leu Thr Lys Val Pro Thr Leu Arg Arg Trp Gly Lys Lys Met Ala 180 185 190 Val Val Val Asp Ala Pro Phe Phe Tyr Ser Met Gly Lys Met Glu Arg 195 200 205 Val Pro His Leu Ser Asn Ala Asp Ile Val Trp Phe Leu Val Asp Phe 210 215 220 Lys Gln Ala Ala Pro Gly Ala Pro Phe Gln Leu Glu Val Val Glu Glu 225 230 235 240 Phe Tyr Thr Thr Leu Glu Ser Ala Thr Leu Gly Leu Thr Gly Gly Val 245 250 255 Pro Val Ser Gln Gly Ala Phe Glu Ala Arg Ile Thr Ala Lys Ala 260 265 270 203292PRTunknownEnterobacter agglomerans 203Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr Ala 50 55 60 Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly Asp 65 70 75 80 Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala Thr 85 90 95 Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu Val 100 105 110 Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile Thr 115 120 125 Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile Asp 130 135 140 Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu Lys 145 150 155 160 Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu Asn 165 170 175 Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys Gly 180 185 190 Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe Ile 195 200 205 Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala Asp 210 215 220 Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His Thr 225 230 235 240 Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln Arg Lys Leu Ile 245 250 255 Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe Ser Lys Arg Phe 260 265 270 Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp Leu Asp Ala Val Ile 275 280 285 Lys Lys Ala Leu 290 204346PRTunknownNocardia otitidiscaviarum 204Ile Ala Glu Phe Phe Gly His Arg Val Tyr Pro Glu Val Val Ser Thr 1 5 10 15 Glu Ala Ala Arg Asn Asp Gln Ala Thr Gly Thr Cys Pro Phe Leu Thr 20 25 30 Ala Ala Lys Leu Val Glu Thr Ser Cys Val Lys Ala Glu Thr Ser Arg 35 40 45 Gly Val Cys Val Val Asn Thr Ala Val Asp Asn Glu Arg Tyr Asp Trp 50 55 60 Leu Val Cys Pro Asn Arg Ala Leu Asp Pro Leu Phe Met Ser Ala Ala 65 70 75 80 Ser Arg Lys Leu Phe Gly Tyr Gly Pro Thr Glu Pro Leu Gln Phe Ile 85 90 95 Ala Ala Pro Thr Leu Ala Asp Gln Ala Val Arg Asp Gly Ile Arg Glu 100 105 110 Trp Leu Asp Arg Gly Val His Val Val Ala Tyr Phe Gln Glu Lys Leu 115 120 125 Gly Gly Glu Leu Ser Ile Ser Lys Thr Asp Ser Ser Pro Glu Phe Ser 130 135 140 Phe Asp Trp Thr Leu Ala Glu Val Glu Ser Ile Tyr Pro Val Pro Lys 145 150 155 160 Ile Lys Arg Tyr Gly Val Leu Glu Ile Gln Thr Met Asp Phe His Gly 165 170 175 Ser Tyr Lys His Ala Val Gly Ala Ile Asp Ile Ala Leu Val Glu Gly 180 185 190 Ile Asp Phe His Gly Trp Leu Pro Thr Pro Ala Gly Arg Ala Ala Leu 195 200 205 Ser Lys Lys Met Glu Gly Pro Asn Leu Ser Asn Val Phe Lys Arg Thr 210 215 220 Phe Tyr Gln Met Ala Tyr Lys Phe Ala Leu Ser Gly His Gln Arg Cys 225 230 235 240 Ala Gly Thr Gly Phe Ala Ile Pro Gln Ser Val Trp Lys Ser Trp Leu 245 250 255 Arg His Leu Ala Asn Pro Thr Leu Ile Asp Asn Gly Asp Gly Thr Phe 260 265 270 Ser Leu Gly Asp Thr Arg Asn Asp Ser Glu Asn Ala Trp Ile Phe Val 275 280 285 Phe Glu Leu Asp Pro Asp Thr Asp Ala Ser Pro Arg Pro Leu Ala Pro 290 295 300 His Leu Glu Ile Arg Val Asn Val Asp Thr Leu Ile Asp Leu Ala Leu 305 310 315 320 Arg Glu Ser Pro Arg Ala Ala Leu Gly Pro Ser Gly Pro Val Ala Thr 325 330 335 Phe Thr Asp Lys Val Glu Ala Arg Met Leu 340 345 205282PRTunknownEnterobacter agglomerans 205Leu Ser Pro Glu Val Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe 1 5 10 15 Leu Asn Lys Glu Cys Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr 20 25 30 Gly Thr Cys Ser Val Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro 35 40 45 Asn Arg Leu Tyr Ala Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg 50 55 60 Asp Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile 65 70 75 80 Lys Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn 85 90 95 Ser Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val 100 105 110 Leu Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu 115 120 125 Ile Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala 130 135 140 Tyr Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser 145 150 155 160 Gln His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln 165 170 175 Ile Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys 180 185 190 Gly Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp 195 200 205 Val Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser 210 215 220 Ile Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu 225 230 235 240 Gln Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu 245 250 255 Phe Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp 260 265 270 Leu Asp Ala Val Ile Lys Lys Ala Leu Gly 275 280 206273PRTunknownSilicibacter pomeroyi DSS-3 206Arg Asp Lys Leu Thr Arg Gln Gln Ala Glu Glu Ile Pro Cys Pro Phe 1 5 10 15 Arg Pro Asp Thr Pro Asn Ala Thr Cys Thr Lys Pro Gly Gly Val Cys 20 25 30 Ser Ile Arg Val Tyr Arg Gly Glu Lys Asn Arg Val Glu Pro Ile Thr 35 40 45 Gly Glu Arg Gly Arg Leu Arg Ala Leu Cys Pro Trp Arg Phe His Gln 50 55 60 Asp Gly Lys Ala Phe Ser Glu Val Gly Lys Arg Leu Leu Asn Asp Pro 65 70 75 80 Asp Pro Ile Lys Ala Gly Glu Val Gly Phe Leu Glu Ser Ser Gly Asn 85 90 95 Leu Asp Ser Asp Pro Gly Glu Asp Val Gly Arg Ile Asp Met Ile Leu 100 105 110 Val Lys Ser Asn Gly Val Glu Gly Ala Pro Met Asp Trp Val Ala Val 115 120 125 Glu Val Gln Ala Val Tyr Phe Ser Gly Lys Lys Met Ser Ile Glu Phe 130 135 140 Asp His Leu Ile Lys Thr Gln Gly Lys Ile Ser Met Ala Arg Glu Lys 145 150 155 160 Arg Arg Pro Asp Tyr Arg Ser Ser Gly Val Lys Arg Leu Met Pro Gln 165 170 175 Leu Gln Thr Lys Val Pro Thr Leu Arg Arg Trp Gly Lys Lys Met Ala 180 185 190 Val Val Val Asp Ala Pro Phe Phe Tyr Ser Met Gly Glu Met Ala Arg 195 200 205 Glu Arg Asp Val Ser Asn Ala Asp Ile Ile Trp Phe Leu Ala Asp Phe 210 215 220 Lys Glu Asp Leu Asn Gly Gly Gly Phe Lys Leu Glu Ile Val Glu Glu 225 230 235 240 Phe Tyr Thr Thr Leu Glu Ser Ala Thr Leu Gly Leu Thr Gly Gly Thr 245 250 255 Pro Val Ser Gln Gly Asp Phe Glu Ala Arg Ile Arg Ala Lys Thr Asp 260 265 270 Gly 207259PRTunknownEnterobacter agglomerans 207Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr Ala 50 55 60 Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly Asp 65 70 75 80 Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala Thr 85 90 95 Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu Val 100 105 110 Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile Thr 115 120 125 Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile Asp 130 135 140 Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu Lys 145 150 155 160 Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu Asn 165 170 175 Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys Gly 180 185 190 Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe Ile 195 200 205 Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala Asp 210 215 220 Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His Thr 225 230 235 240 Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln Arg Lys Leu Ile 245 250 255 Ser Glu Arg 208238PRTunknownXylella fastidiosa Ann-1 208Val Val Glu Leu Phe Gly Lys Ala Ala Asp Ala Pro Gly Ile Asp Trp 1 5 10 15 Gln Asn Glu Ile Ala Asp Gln Gln Cys Pro Phe Leu Gly Lys Arg Cys 20 25 30 Tyr Lys Val Arg Lys Ser Asn Pro Glu Ile Ser Ile Gly Ser Cys Thr 35 40 45 Val Leu Tyr Gly Arg Glu Pro Glu Pro Ile Ile Ile Cys Pro Thr Arg 50 55 60 Leu Ile Gln Arg Gly Gln Ile Phe Thr Asp Cys Leu His Leu Leu Thr 65 70 75 80 Ser His Glu Pro Gly Asn Glu Leu His Leu Val Ser Glu Val Thr Val 85 90 95 Pro Gly Gly Ser Ile Asp Tyr Val Leu Val Ser Ala Lys Glu Gly Lys 100 105 110 Val Arg Asp Phe Val Gly Ile Glu Leu Gln Thr Leu Asp Thr Thr Gly 115 120 125 Thr Val Trp Pro Glu Arg Gln Arg Leu Leu Lys Glu Leu Gly Val Ala 130 135 140 Arg Gly Asp Asn Gly Glu Glu Ser Asp Lys Ser Phe Gly Met Asn Trp 145 150 155 160 Lys Met Thr Ala Lys Thr Ile Leu Val Gln Met His His Lys Val Gln 165 170 175 Thr Phe Glu His Val Asn Arg Lys Leu Val Leu Val Val Gln Asp Lys 180 185 190 Phe Leu Ala Tyr Met Thr Lys Glu Phe Lys Phe Asp His Met Lys Asn 195 200 205 Pro Ala Ala Val Gly Asp Ser Met His Leu His Ser Tyr Arg Met Ala 210 215 220 Arg Ala Asp Asp Gly Asn Phe Arg Leu Ser Met Ala Ser Arg 225 230 235 209259PRTunknownEnterobacter agglomerans 209Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr Ala 50 55 60 Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly Asp 65 70 75 80 Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala Thr 85 90 95 Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu Val 100 105 110 Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile Thr 115 120 125 Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile Asp 130 135 140 Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu Lys 145 150 155 160 Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu Asn 165 170 175 Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys Gly 180 185 190 Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe Ile 195 200 205 Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala Asp 210 215 220 Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His Thr 225 230 235 240 Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln Arg Lys Leu Ile 245 250 255 Ser Glu Arg 210238PRTunknownXylella fastidiosa 9a5c 210Val Val Glu Leu Phe Gly Lys Ala Ala Asp Ala Pro Gly Ile Asp Trp 1 5 10 15 Gln Asn Glu Ile Ala Asp Gln Gln Cys Pro Phe Leu Gly Lys Arg Cys 20 25 30 Tyr Asn Val Arg Lys Ser Asn Pro Glu Ile Ser Ile

Gly Ser Cys Thr 35 40 45 Val Leu Tyr Gly Arg Glu Pro Glu Pro Ile Ile Ile Cys Pro Thr Arg 50 55 60 Leu Ile Gln Arg Gly Gln Ile Phe Thr Asp Cys Leu His Leu Leu Thr 65 70 75 80 Ser His Glu Pro Gly Asn Glu Leu His Leu Val Ser Glu Val Thr Val 85 90 95 Pro Gly Gly Ser Ile Asp Tyr Val Leu Val Ser Ala Lys Glu Gly Lys 100 105 110 Val Arg Asp Phe Val Gly Ile Glu Leu Gln Thr Leu Asp Thr Thr Gly 115 120 125 Thr Val Trp Pro Glu Arg Gln Arg Leu Leu Lys Glu Leu Gly Val Ala 130 135 140 Arg Gly Asp Asn Gly Glu Glu Ser Asp Lys Ser Phe Gly Met Asn Trp 145 150 155 160 Lys Met Thr Ala Lys Thr Ile Leu Val Gln Met His His Lys Val Gln 165 170 175 Thr Phe Glu His Val Asn Arg Lys Leu Val Leu Val Val Gln Asp Lys 180 185 190 Phe Leu Ala Tyr Met Thr Lys Glu Phe Lys Phe Asp His Met Lys Asn 195 200 205 Pro Ala Ala Val Gly Asp Ser Met His Leu His Ser Tyr Arg Met Ala 210 215 220 Arg Ala Asp Asp Gly Asn Phe Arg Leu Ser Met Ala Ser Arg 225 230 235 211290PRTunknownEnterobacter agglomerans 211Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr Ala 50 55 60 Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly Asp 65 70 75 80 Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala Thr 85 90 95 Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu Val 100 105 110 Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile Thr 115 120 125 Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile Asp 130 135 140 Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu Lys 145 150 155 160 Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu Asn 165 170 175 Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys Gly 180 185 190 Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe Ile 195 200 205 Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala Asp 210 215 220 Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His Thr 225 230 235 240 Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln Arg Lys Leu Ile 245 250 255 Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe Ser Lys Arg Phe 260 265 270 Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp Leu Asp Ala Val Ile 275 280 285 Lys Lys 290 212299PRTunknownBurkholderia cepacia 212Ile Gly Glu Trp Phe Gly Phe Asn Leu Thr Gln Leu Ser Gly Glu Glu 1 5 10 15 Arg Arg Gln Leu Ala Ala Glu Val Leu Lys Pro Lys Lys Glu Arg Thr 20 25 30 Pro Gln Pro Cys Pro Phe Gln Ala Arg Lys Thr Gly Ala Val Cys Ser 35 40 45 Lys Asp Gly Gly Val Cys Ser Leu Arg Leu Tyr Ser Tyr Asn Thr His 50 55 60 Pro Asp Asn Gly Arg Ala Val Gly Val Pro Val Glu Gly Lys Gln Gly 65 70 75 80 Asp Leu Arg Ala Thr Cys Pro Tyr Arg Phe His Asp Glu Leu Asp Val 85 90 95 Phe Lys Trp Val Gly Glu Thr Ile Leu Gly Asp Pro Asp Pro Leu Leu 100 105 110 Val Gly Glu Val Gly Phe Leu Glu Ala Gly Ala Ser Thr Asp Ser Glu 115 120 125 Gly Gly Asp Asp Val Gly Arg Ile Asp Met Val Leu Val Ser Ser Lys 130 135 140 Thr Pro Lys Glu Ala Pro Met Asn Trp Ala Ala Leu Glu Ile Gln Ala 145 150 155 160 Val Tyr Phe Ser Gly Asn Ala Met Lys Gly Glu Phe Glu Ala Phe Asn 165 170 175 Asp Asp Ala Val Asp Trp Val Ile Phe Pro Ala Gly Arg Arg Arg Pro 180 185 190 Asp Tyr Arg Ser Ser Gly Pro Lys Arg Leu Met Pro Gln Leu Gln Ile 195 200 205 Lys Val Pro Thr Leu Arg Arg Trp Gly Lys Lys Met Ala Val Val Val 210 215 220 Asp Arg Ala Phe Phe Asp Ser Ile Gly Glu Met Asp Asn Val Ala Asp 225 230 235 240 Ile Ser Asn Ala Asp Ile Ala Trp Phe Ile Val Arg Phe Glu Glu Val 245 250 255 Glu Gly Gln Lys Arg Thr Arg Ile Val Arg Asp Glu Val Arg Tyr Thr 260 265 270 Thr Leu Glu Arg Ser Val Glu Gly Leu Thr Gly Gly Lys Pro Val Pro 275 280 285 Leu Pro Val Phe Glu Thr Arg Ile Thr Asp Lys 290 295 213190PRTunknownEnterobacter agglomerans 213Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp 50 55 60 Val Leu Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val 65 70 75 80 Glu Ile Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His 85 90 95 Ala Tyr Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr 100 105 110 Ser Gln His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro 115 120 125 Gln Ile Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys 130 135 140 Lys Gly Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu 145 150 155 160 Asp Val Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys 165 170 175 Ser Ile Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala 180 185 190 214229PRTunknownArthrospira platensis 214Ile Ile Glu Ile Phe Gly Tyr Ser Ile Asn Gln Pro Glu His Ile Asp 1 5 10 15 Trp Thr Ser Leu Ile Arg Glu Gln His Cys Pro Tyr Leu Gln Arg Arg 20 25 30 Cys Ile Lys Val Arg Lys Ser Gln Pro Asp Ile Ser Ile Gly Thr Cys 35 40 45 Ser Val Ile Tyr Gly Lys Asn Ala Ile Pro Val Ile Ile Cys Pro His 50 55 60 Arg Leu Leu Glu Arg Lys Gln Ile Phe Ile Asp Cys Leu His Leu Leu 65 70 75 80 Thr Asn His Glu Pro Gly Asn Glu Leu His Leu Val Ser Glu Ile Ser 85 90 95 Ile Pro Gly Gly Asn Val Asp Tyr Phe Leu Val Ser Ala Leu Asn Asn 100 105 110 Lys Val Lys Asp Phe Val Gly Ile Glu Leu Gln Thr Leu Asp Thr Thr 115 120 125 Gly Thr Val Trp Pro Glu Arg Gln Arg Leu Leu Glu Glu Leu Gly Val 130 135 140 Pro Thr Glu Asp Asn Gln Ser Gln Ser Gln Lys Thr Phe Gly Met Asn 145 150 155 160 Trp Lys Met Thr Ala Lys Thr Ile Leu Ile Gln Leu His His Lys Ile 165 170 175 Glu Thr Phe Glu His Ile Asn Lys Lys Leu Val Leu Val Ile Gln Asp 180 185 190 Cys Phe Leu Asp Tyr Ile Gln Arg Glu Phe Ser Phe Ser His Ile Ser 195 200 205 His Gln Ala Gln Leu Gly Asp Ser Met His Ile His Ala Tyr Gln Met 210 215 220 Thr Glu Gln Pro Asp 225 215248PRTunknownEnterobacter agglomerans 215Leu Val Glu Val Phe Gly Tyr Asn Pro Met Asp Leu Ser Pro Glu Val 1 5 10 15 Arg Ala Leu Trp Asn Leu Gly Ala Cys Pro Phe Leu Asn Lys Glu Cys 20 25 30 Ile Lys Ile Asn His Asp Gln Thr Ile Ile Tyr Gly Thr Cys Ser Val 35 40 45 Thr Ser Pro Tyr Gly Asp Val Ile Ile Cys Pro Asn Arg Leu Tyr Ala 50 55 60 Asn Asp Tyr Glu Thr Leu His Lys Val Ser Arg Asp Ala Phe Gly Asp 65 70 75 80 Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys Tyr Arg Ala Thr 85 90 95 Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser Gly Lys Glu Val 100 105 110 Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu Val Arg Ile Thr 115 120 125 Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile Gln Ser Ile Asp 130 135 140 Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr Lys Asn Leu Lys 145 150 155 160 Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln His Gly Leu Asn 165 170 175 Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile Ile Arg Lys Gly 180 185 190 Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly Leu Tyr Phe Ile 195 200 205 Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val Ile Gly Ala Asp 210 215 220 Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile Thr Val His Thr 225 230 235 240 Tyr Ser Leu Gly Glu Pro Ala Ala 245 216226PRTunknownNostoc punctiforme PCC 73102 216Val Val Glu Leu Tyr Gly Asn Pro Thr Asn Gln Ser Leu Ile Trp Ser 1 5 10 15 Asp Ile Ala Ser Ser Gln Asn Cys Pro Phe Leu Ser Arg Lys Cys Leu 20 25 30 Lys Asn Arg Lys Ser Glu Pro Asp Leu Thr Ile Gly Ser Cys Thr Val 35 40 45 Ser Tyr Gly Arg Glu Ala Arg Asn Ile Ile Ile Cys Pro Phe Arg Leu 50 55 60 Leu Glu Arg Ser Gln Ile Phe Thr Asp Cys Ile His Leu Leu Thr Leu 65 70 75 80 His Glu Pro Gly Asn Glu Leu Arg Ile Val Pro Glu Ile Ala Val Pro 85 90 95 Gly Gly Ser Ile Asp Tyr Cys Leu Ala Ser Val Arg Ser Gly Lys Val 100 105 110 Ile Asp Phe Ile Ser Ile Glu Leu Gln Thr Leu Asp Thr Thr Gly Thr 115 120 125 Val Trp Pro Glu Arg Gln Arg Phe Leu Gln Arg His Gly Val Ser Val 130 135 140 Arg Asp Val Asp Val Ala Ser Gly Lys Gly Phe Gly Met Asn Trp Lys 145 150 155 160 Met Thr Ala Lys Thr Ile Leu Met Gln Leu His His Lys Ile His Thr 165 170 175 Phe Glu His Leu Ser Lys His Leu Val Leu Val Val Gln Asp Cys Leu 180 185 190 Ile Asp Tyr Met Gln Arg Glu Phe Ser Phe Glu His Ile Gln Asp Ala 195 200 205 Arg Leu Gly Asn Pro Met His Phe His Ser Tyr Glu Leu Leu Thr Glu 210 215 220 Ala Ser 225 217216PRTunknownEnterobacter agglomerans 217Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys 1 5 10 15 Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser 20 25 30 Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu 35 40 45 Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile 50 55 60 Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr 65 70 75 80 Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln 85 90 95 His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile 100 105 110 Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly 115 120 125 Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val 130 135 140 Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln Thr Asn Lys Ser Ile 145 150 155 160 Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln 165 170 175 Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe 180 185 190 Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys Gly Asp Asp Leu 195 200 205 Asp Ala Val Ile Lys Lys Ala Leu 210 215 218201PRTunknownSyntrophomonas wolfei str. Goettingen 218Met Val Thr Ile Asp Ala Ala Ser Phe Phe Phe Ser Pro Gly Thr Lys 1 5 10 15 Trp Thr Thr Leu Thr Glu Val Arg Leu Asn Asp Ile Asn Gly His Thr 20 25 30 Ala Gly Asn Ile Asp Ile Val Leu Val Ala Tyr Asp Asp Tyr Gly Lys 35 40 45 Ile Thr Asp Phe Gly Ala Leu Glu Ile Gln Ser Val Tyr Ile Ser Gly 50 55 60 Asn Ile Arg Arg Pro Phe Glu Ala Tyr Ile Gln Glu Pro Glu Leu Met 65 70 75 80 Tyr Asn Met Asp Trp Leu Ser Lys Pro Asn Tyr Pro Arg Pro Asp Tyr 85 90 95 Leu Ser Ser Ser Arg Lys Arg Leu Val Pro Gln Leu Ile Tyr Lys Gly 100 105 110 Lys Ile Leu Asn Val Trp Ser Lys Lys Ile Ala Val Ala Leu His Ser 115 120 125 Gly Phe Phe Ser Thr Leu Pro Gln Leu Pro Arg Val Ser Ala Asp Lys 130 135 140 Ala Glu Ile Ala Trp Leu Ile Tyr Asp Ile Glu Leu Lys Gln Glu Thr 145 150 155 160 Asn Arg Tyr Asn Leu Val His Thr Asp Thr Ile Tyr Thr Leu Phe Gln 165 170 175 Asn Ser Leu Asp Arg Ile Val Thr Pro Glu Ser Gly Leu Ile Asp Asp 180 185 190 Phe Ile Glu Val Leu Gln Gly Lys Leu 195 200 219204PRTunknownEnterobacter agglomerans 219Ala Phe Gly Asp Asp Val Pro Phe Leu Thr Tyr Ser Asn Phe Ile Lys 1 5 10 15 Tyr Arg Ala Thr Tyr Lys Asp Cys Ile Val Ala Leu Gly Lys Asn Ser 20 25 30 Gly Lys Glu Val Gln Val Gly Arg Ala Leu Ser Met Asp Trp Val Leu 35 40 45 Val Arg Ile Thr Asp Gly Glu Leu Lys Glu Tyr Val Gly Val Glu Ile 50 55 60 Gln Ser Ile Asp Ile Thr Gly Asn Tyr Arg Asp Ala Trp His Ala Tyr 65 70 75 80 Lys Asn Leu Lys Pro Ile Asp Ile Ile Asp Asn Leu Pro Thr Ser Gln 85 90 95 His Gly Leu Asn Trp Ala Asn Val His Lys Arg Leu Ile Pro Gln Ile 100 105 110 Ile Arg Lys Gly Val Val Tyr Ser Arg Ser Asn Tyr Val Lys Lys Gly 115 120 125 Leu Tyr Phe Ile Leu Pro Glu Ile Val Tyr Asn Lys Phe Glu Asp Val 130 135 140 Ile Gly Ala Asp Ile Pro Leu Leu Lys Thr Gln

Thr Asn Lys Ser Ile 145 150 155 160 Thr Val His Thr Tyr Ser Leu Gly Glu Pro Ala Ala Asn Gly Glu Gln 165 170 175 Arg Lys Leu Ile Ser Glu Arg Glu Ile Ile Phe Asp Leu Asp Glu Phe 180 185 190 Ser Lys Arg Phe Thr Thr Gly Pro Asn Leu Pro Lys 195 200 220192PRTunknownChlorobium phaeobacteroides DSM 266 220Met Ile Thr Asp Asp Ala Ala Ser Phe Phe Phe Asp Glu Ser Thr Thr 1 5 10 15 Trp Ser Ser Leu Thr Glu Val Arg Leu Asn Asp Ala Tyr Gly Lys Ser 20 25 30 Ala Gly Asn Thr Asp Val Val Leu Val Ala Tyr Asp Lys Thr Gly Lys 35 40 45 Val Ile Asp Phe Gly Ala Leu Glu Ile Gln Ala Val Tyr Ile Ser Gly 50 55 60 Asn Val Arg Glu Pro Phe Glu Gln Phe Met Lys Glu Pro Glu Thr His 65 70 75 80 Glu Asn Met Asn Trp Thr Thr Gln Pro Asn Tyr Pro Arg Pro Asp Tyr 85 90 95 Leu Ser Ser Ser Arg Lys Arg Leu Ala Pro Gln Leu Leu Phe Lys Gly 100 105 110 Gly Ile Leu Asn Ile Arg Lys Lys Lys Thr Ala Val Ala Ile Asn Lys 115 120 125 Ser Phe Phe Asp Thr Leu Pro Ser Phe Lys Gln Val Glu Lys Ser Lys 130 135 140 Ala Thr Ile Ala Trp Ile Val Tyr Asp Leu Glu Leu Ser Asp Glu Asp 145 150 155 160 Gly Leu Glu Arg Tyr His Leu Lys Lys Ile Asp Glu Val Tyr Thr Glu 165 170 175 Phe Glu Pro Ala Leu Val Ala Ile Thr Thr Ala Thr Pro Gly Lys Arg 180 185 190

Claims

1. An isolated protein having at least 90% amino acid sequence identity with SEQ ID NO: 104.

2. An isolated protein having an amino acid sequence identified by an Expectation value of less than or equal to e-05 in a BLAST search for detecting sequence similarity using the amino acid sequence of SEQ ID NO: 104.

3. The isolated protein according to claim 2, having restriction endonuclease activity.

4. A method for identifying a restriction endonuclease, comprising: (a) selecting one or more probes having at least 90% sequence identity to a sequence selected from SEQ ID NO:104; (b) comparing the one or more probes with a database of sequences by a sequence similarity analysis to identify a sequence match; and (c) identifying the restriction endonuclease from the sequence match.

5. A method according to claim 4, wherein the sequence is an amino acid sequence and the sequence similarity analysis comprises a BLAST search using an expectation value, E, of less than or equal to e-02.

6. A method according to claim 4, wherein the sequence is an amino acid sequence and the sequence similarity analysis is a PSI-BLAST search using an expectation value, E, of less than or equal to e-02.

7. A method for identifying a restriction endonuclease, comprising: (a) selecting one or more probes having at least 90% sequence identity to a known restriction endonuclease sequence; (b) comparing the one or more probes with a database of sequences by a sequence similarity analysis to identify a sequence match, wherein the sequence match results from a sequence producing an expectation value, E, of less than or equal to e-02; and (c) identifying the restriction endonuclease from the sequence match.

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