NITRATE TRANSPORT COMPONENTS

Abstract

This invention relates to isolated nucleic acid fragments encoding high affinity nitrate transport components. The invention also relates to the construction of recombinant DNA constructs encoding all or a portion of nitrate transport components, in sense or antisense orientation, wherein expression of the recombinant DNA construct may alter levels of the nitrate transport components in a transformed host cell.

Description

FIELD OF THE INVENTION

[0001] This invention is in the field of plant molecular biology. More specifically, this invention pertains to nucleic acid fragments encoding high affinity nitrate transporters in plants and seeds.

BACKGROUND OF THE INVENTION

[0002] Higher plants are autotrophic organisms that can synthesize all of their molecular components from inorganic nutrients obtained from the local environment. Nitrogen is a key element in many compounds present in plant cells.

[0003] It is found in the nucleoside phosphates and amino acids that form the building blocks of nucleic acids and proteins, respectively. Availability of nitrogen for crop plants is an important limiting factor in agricultural production, and the importance of nitrogen is demonstrated by the fact that only oxygen, carbon, and hydrogen are more abundant in higher plant cells. Nitrogen present in the form of ammonia or nitrate is readily absorbed and assimilated by higher plants.

[0004] Nitrate is the principal source of nitrogen that is available to higher plants under normal field conditions. Thus, the nitrate assimilation pathway is the major point of entry of inorganic nitrogen into organic compounds (Hewitt et al. (1976) Plant Biochemistry, pp 633-6812, Bonner, and Varner, eds. Academic Press, NY). Although some plants directly utilize ammonia, under certain conditions, nitrate is generally the major form of nitrogen available to plants.

[0005] Nitrate uptake by root cells is the first step of the nitrate assimilation pathway in higher plants (Orsel et al. (2002) Plant Physiology 129: 886-896). Plants have developed two different uptake systems to cope with the varying availability of nitrate in cultivated soils. The low-affinity nitrate transport system is used preferentially when external nitrate concentration is high, whereas the high-affinity transport system (HATS) takes place at very low external concentrations.

[0006] In higher plants, two gene families have been identified: the NRT1 and NRT2 families involved in the low-affinity transport system and HATs, respectively. The complexity of nitrate/nitrite transport is enhanced by the fine regulation that occurs at the transcriptional level: both low and high-affinity systems have constitutive and inducible components that are clearly distinct. Furthermore, some members of the nitrate transporters require a second gene product, a NAR2-type polypeptide for function (Tong et al. (2005) The Plant Journal 41: 442-450).

[0007] The nucleotide sequences of the instant application and the methods of their use can increase the efficiency by which nitrogen can be used.

SUMMARY OF THE INVENTION

[0008] The present invention includes isolated polynucleotides encoding a polypeptide required for high affinity nitrate transport, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO: 36 or 49, have at least 80%, 85%, 90%, 95%, 99% or 100% identity (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary. The polypeptide preferably comprises the amino acid sequence of SEQ ID NO: 36 or 49. The nucleotide sequence preferably comprises the nucleotide sequence of SEQ ID NO: 35 or 48.

[0009] In a first embodiment, the present invention includes an isolated polynucleotide comprising: (a) a nucleotide sequence encoding a polypeptide required for high affinity nitrate transport, wherein the polypeptide has an amino acid sequence of at least 80%, 85%, 90%, 95%, 99% or 100% sequence identity based on the Clustal V method of alignment when compared to a polypeptide SEQ ID NO: 36 or 49.

[0010] (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.

[0011] In a second embodiment, this invention concerns such isolated nucleotide sequence or its complement which comprises at least two motifs corresponding substantially to any of the amino acid sequences set forth in SEQ ID NO: 50, 51 or 52, wherein said motif is substantially a conserved subsequence. Examples of such motifs, among others that can be identified, are shown in SEQ ID NO: 50, 51 or 52. Also of interest is the use of such fragment or a part thereof in antisense inhibition or co-suppression in a transformed plant.

[0012] In a third embodiment this invention concerns such isolated nucleotide fragment complement thereof wherein the fragment or a part thereof is useful in antisense inhibition or co-suppression of a protein altering nitrate transport in a transformed plant.

[0013] In a fourth embodiment, this invention concerns an isolated nucleic acid fragment comprising a promoter wherein said promoter consists essentially of the nucleotide sequence set forth in SEQ ID NO:: 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73, 74, 89 or 90, or said promoter consists essentially of a fragment or subfragment that is substantially similar and functionally equivalent to the nucleotide sequence set forth in SEQ ID NO:: 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73, 74, 89 or 90.

[0014] In a fifth embodiment, this invention concerns recombinant DNA constructs comprising any of the foregoing nucleic acid fragment or complement thereof or part of either operably linked to at least one regulatory sequence. Also, of interest are plants comprising such recombinant DNA constructs in their genome, plant tissue or cells obtained from such plants and seeds obtained from these plants.

[0015] In a sixth embodiment, this invention concerns a method of altering nitrate transport in plants which comprises:

[0016] (a) transforming a plant with a recombinant DNA construct comprising:

[0017] i) a first recombinant DNA construct comprising an isolated polynucleotide encoding a HAT polypeptide, operably linked to at least one regulatory sequence; and

[0018] ii) at least one additional recombinant DNA construct comprising an isolated polynucleotide encoding a NAR polypeptide, operably linked to at least one regulatory sequence,

[0019] (b) growing the transformed plant of (a) under conditions suitable for the expression of the recombinant DNA constructs; and selecting those transformed plants having altered nitrate transport. Corn plants comprising these recombinant constructs are also part of this invention.

[0020] In a seventh embodiment, this invention concerns a method to isolate nucleic acid fragments encoding polypeptides associated with altering nitrate transport which comprises:

[0021] (a) comparing SEQ ID NO: 36, 49, 55, or 58 with other polypeptide sequences associated with altering plant nitrate transport;

[0022] (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);

[0023] (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b); and

[0024] (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering nitrate transport by sequence dependent protocols.

[0025] In an eighth embodiment, this invention also concerns a method of mapping genetic variations related to altering plant nitrate transport:

[0026] (a) crossing two plant varieties; and

[0027] (b) evaluating genetic variations with respect to:

[0028] (i) a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 35, 48, 54, and 57; or

[0029] (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs: 36, 49, 55, and 58;

[0030] in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.

[0031] In a ninth embodiment, this invention concerns a method of molecular breeding to obtain altered plant nitrate transport, comprising:

[0032] (a) crossing two plant varieties; and

[0033] (b) evaluating genetic variations with respect to:

[0034] (i) a nucleic acid sequence selected from the group consisting of SEQ ID NOs:35, 48, 54, and 57; or

[0035] (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs: 36, 49, 55, and 58;

[0036] in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.

[0037] In a tenth embodiment, this invention concerns a method of altering the level of expression of a high affinity nitrate transporter polypeptide in a host cell comprising: (a) transforming a host cell with a recombinant DNA construct comprising:

[0038] (b) a nucleotide sequence encoding a high affinity nitrate transporter polypeptide, wherein the polypeptide has an amino acid sequence of at least 80% sequence identity, based on the Clustal V method of alignment, when compared to one of SEQ ID NO: 36 or 49 and the polypeptide alters nitrate transport, the complement thereof or at least two motifs corresponding substantially to any of the amino acid sequences set forth in SEQ ID NOs: 50, 51 and 52, wherein said motif is a substantially conserved subsequence operably linked to at least one regulatory sequence; and

[0039] (c) growing the transformed host cell under conditions that are suitable for expression of the recombinant DNA construct wherein expression of the recombinant DNA construct results in production of altered levels of the polypeptide required for nitrate transport in the transformed host cell.

[0040] In an eleventh embodiment, this invention concerns a corn plant, comprising a first DNA construct comprising an isolated HAT polypeptide, operably linked to at least one regulatory sequence; and at least one additional recombinant DNA construct comprising an isolated polynucleotide, operably linked to at least one regulatory sequence, encoding a polypeptide selected from the group consisting of a NAR 2.

[0041] An additional embodiment of this invention concerns a method for altering plant nitrogen transport, comprising:

[0042] (a) transforming a plant with a recombinant DNA construct comprising:

[0043] i) a first recombinant DNA construct comprising an isolated polynucleotide encoding a HAT polypeptide, operably linked to at least one regulatory sequence; and

[0044] ii) at least one additional recombinant DNA construct comprising an isolated polynucleotide, operably linked to at least one regulatory sequence, encoding a polypeptide selected from the group consisting of a NAR;

[0045] (b) growing the transformed plant of (a) under conditions suitable for the expression of the recombinant DNA construct; and

[0046] (c) selecting those transformed plants having altered nitrate transport.

[0047] Further embodiments of this invention include shuffled HAT variants with improved kinetic parameters, recombinant DNA constructs comprising the nucleotide sequences encoding these variants and plants and transformed cells comprising in their genome these recombinant DNA construct. Also included in this invention are corn plants comprising a first recombinant DNA construct comprising a nucleotide sequence encoding a shuffled HAT variant, operably linked to at least one regulatory sequence and at least one additional recombinant DNA construct comprising an isolated polynucleotide, operably linked to at least one regulatory sequence, encoding a polypeptide selected from the group consisting of a NAR.

[0048] Yet another embodiment of this invention sets forth a method for altering plant nitrogen transport, comprising: a) transforming a plant with a recombinant DNA construct comprising a first recombinant DNA construct comprising a nucleotide sequence encoding a shuffled HAT variant, operably linked to at least one regulatory sequence and at least one additional recombinant DNA construct comprising an isolated polynucleotide, operably linked to at least one regulatory sequence, encoding a polypeptide selected from the group consisting of a NAR; and b) growing the transformed plant of (a) under conditions suitable for the expression of the recombinant DNA construct; and selecting those transformed plants having altered nitrate transport.

Biological Deposits

[0049] The following plasmids have been deposited with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209, and bear the following designations, accession numbers and dates of deposit.

TABLE-US-00001 Plasmid Accession Number Date of Deposit PHP27621 ATCC

BRIEF DESCRIPTION OF THE SEQUENCE LISTINGS

[0050] The invention can be more fully understood from the following detailed description and the accompanying drawings and Sequence Listing, which form a part of this application.

[0051] FIG. 1 is a schematic of vector PHP27621.

[0052] FIG. 2 is a schematic of vector PHP27660.

[0053] FIG. 3 is a schematic of vector PHP27860.

[0054] FIG. 4 is a schematic of vector PHP27280.

[0055] FIG. 5 is a schematic of vector PHP27281.

[0056] FIG. 6 is a schematic of vector PHP27282.

[0057] FIG. 7 is a schematic of vector PHP27283.

[0058] SEQ ID NO: 1 is the forward primer used in Example 3.

[0059] SEQ ID NO: 2 is the reverse primer used in Example 3.

[0060] SEQ ID NO: 3 is the T7 primer used in Example 3 for confirmatory BAC ends sequencing.

[0061] SEQ ID NO: 4 is the SP6 primer used in Example 3 for confirmatory BAC ends sequencing.

[0062] SEQ ID NO: 5 through 33 are the sequencing primers used to cover the region on BAC clone bacc.pk139.d24 containing the HAT4 gene.

[0063] SEQ ID NO: 34 represents the 3924 bp of the maize genomic sequence containing the ORF (Nucleotides 2015-3583 (Stop)) of the gene encoding the high affinity nitrate transporter (HAT4) isolated from BAC clone bacc.pk139.d24.

[0064] SEQ ID NO: 35 is 1569 bp of the nucleotide sequence of the ORF of SEQ ID NO: 34.

[0065] SEQ ID NO: 36 is the amino acid sequence encoded by nucleotides 2015-3580 of SEQ ID NO: 34.

[0066] SEQ ID NO: 37 is the 2014 bp, extending from Nucleotides 1-2014 of the putative promoter of the maize high affinity nitrate transporter genomic sequence shown in SEQ ID NO: 34.

[0067] SEQ ID NO: 38 is 1014 bp, extending from Nucleotide 1001-2014 of the putative promoter of the maize high affinity nitrate transporter genomic sequence shown in SEQ ID NO: 34.

[0068] SEQ ID NO: 39-42 are the forward and reverse primers used in Example 4.

[0069] SEQ ID NO: 43 is the T3 primer used in Example 4.

[0070] SEQ ID NO: 44 is the T7 primer used in Example 4.

[0071] SEQ ID NO: 45 represents the 5812 bp of the maize genomic sequence containing the ORF (Nucleotides 2264-3450 and 5087-5357 (Stop)) of the gene encoding a high affinity nitrate transporter (HAT7).

[0072] SEQ ID NO: 46 is the 2263 bp, extending from Nucleotides 1-2263 of the putative promoter of the maize high affinity nitrate transporter genomic sequence shown in SEQ ID NO: 45.

[0073] SEQ ID NO: 47 is the 1263 bp, extending from Nucleotides 1001-2263 of the putative promoter of the maize high affinity nitrate transporter genomic sequence shown in SEQ ID NO: 45.

[0074] SEQ ID NO: 48 is 1455 bp of the coding sequence, extending from Nucleotides 2264-3450 and 5087-5354 of SEQ ID NO: 45.

[0075] SEQ ID NO: 49: is the amino acid sequence encoded by SEQ ID NO: 48.

[0076] SEQ ID NO: 50 is a conserved sequence motif useful in identifying genes belonging to the high affinity nitrate transporter of genes.

[0077] SEQ ID NO: 51 is a conserved sequence motif useful in identifying genes belonging to the high affinity nitrate transporter of genes.

[0078] SEQ ID NO: 52 is a conserved sequence motif useful in identifying genes belonging to the high affinity nitrate transporter of genes.

[0079] SEQ ID NO: 53 is the 1561 bp of the sequence containing the ORF (nucleotides 757-1368 (Stop)) encoding a corn NAR2-type polypeptide (NAR2.1).

[0080] SEQ ID NO: 54 is the 612 bp of the coding sequence, extending from nucleotides 758-1369 (Stop) of SEQ ID NO: 53.

[0081] SEQ ID NO: 55 is the amino acid sequence encoded by nucleotides 758-1366 of SEQ ID NO: 54.

[0082] SEQ ID NO: 56 is the 756 bp, extending from Nucleotides 1-756 of the putative promoter of the sequence shown in SEQ ID NO: 53.

[0083] SEQ ID NO: 57 is the 594 bp of the ORF (nucleotides 1-594 (Stop)) encoding a NAR2-type polypeptide (NAR2.2).

[0084] SEQ ID NO: 58 is the amino acid sequence encoded by nucleotides 1-591 of the ORF of SEQ ID NO: 57.

[0085] SEQ ID NO: 59 is the NAR2.1 specific outer primer used in Example 6.

[0086] SEQ ID NO: 60 is the NAR2.1 specific inner primer used in Example 6.

[0087] SEQ ID NO: 61-64 are the sequencing primers used to sequence the NAR2.1 promoter upstream region.

[0088] SEQ ID NO: 65 shows an additional 2917 bp of the putative NAR2.1 promoter.

[0089] SEQ ID NO: 66 shows the 4498 bp of the complete NAR2.1 gene, including an intron extending from nucleotides 3655-3841.

[0090] SEQ ID NO: 67 is the 3506 bp, extending from Nucleotides 1-3506 of the putative promoter of the NAR2.1 genomic sequence shown in SEQ ID NO: 66.

[0091] SEQ ID NO: 68 is 1014 bp, extending from Nucleotide 1001-2014 of the putative promoter of the NAR2.1 genomic sequence shown in SEQ ID NO: 66.

[0092] SEQ ID NO: 69 is 1492 bp, extending from Nucleotide 2015-3506 of the putative promoter of the NAR2.1 genomic sequence shown in SEQ ID NO: 66.

[0093] SEQ ID NO: 70 is 3621 bp of the genomic fragment isolated in Example 14.

[0094] SEQ ID NO: 71 is 3236 bp of the putative Nar promoter from B73, extending from Nucleotides 1-3236 of SEQ ID NO: 70.

[0095] SEQ ID NO: 72 is 1000 bp of the putative Nar promoter from B73, extending from Nucleotides 1-1000 of SEQ ID NO: 70.

[0096] SEQ ID NO: 73 is 2236 bp of the putative Nar promoter from B73, extending from Nucleotides 1001-3236 of SEQ ID NO: 70.

[0097] SEQ ID NO: 74 is 1237 bp of the putative Nar promoter from B73, extending from Nucleotides 2000-3236 of SEQ ID NO: 70.

[0098] SEQ ID NO: 75 through 78 are the forward and reverse primers described in Example 14.

[0099] SEQ ID NO: 79-84 are the sequencing primers used to sequence the Nar promoter from B73 as described in Example 14.

[0100] SEQ ID NO: 85 is the sequence of vector pENTR-5' described in Example 14.

[0101] SEQ ID NO: 86 is the sequence of vector PHP27621 described in Example 16.

[0102] SEQ ID NO: 87 is the sequence of vector PHP27660 described in Example 17.

[0103] SEQ ID NO: 88 is the sequence of vector PHP27860 described in Example 17.

[0104] SEQ ID NO: 89 is 3324 bp of the putative Nar promoter from B73, comprising Nucleotides 1-1523 and 1821-3324 of SEQ ID NO: 70.

[0105] SEQ ID 90: is 500 bp of the putative Nar promoter from B73, extending from Nucleotides 2825-3324 of SEQ ID NO: 70.

[0106] SEQ ID NO:91: represents the 2025 bp of the maize sequence containing the ORF (Nucleotides 250-1812(Stop)) of the gene encoding the high affinity nitrate transporter (HAT5) isolated from clone cfp4n.pk008.p6:fis.

[0107] SEQ ID NO:92 is the amino acid sequence encoded by the ORF of SEQ ID NO: 91.

[0108] SEQ ID NO: 93 is the sequence of vector PHP27280 described in Example 20.

[0109] SEQ ID NO: 94 is the sequence of vector PHP27281 described in Example 20.

[0110] SEQ ID NO: 95 is the sequence of vector PHP27282 described in Example 20.

[0111] SEQ ID NO: 96 is the sequence of vector PHP27283 described in Example 20.

[0112] The Sequence Listing contains the one letter code for nucleotide sequence characters and the three letter codes for amino acids as defined in conformity with the IUPAC-IUBMB standards described in Nucleic Acids Research 13:3021-3030 (1985) and in the Biochemical Journal 219 (No. 2): 345-373 (1984) which are herein incorporated by reference. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.

DETAILED DESCRIPTION OF THE INVENTION

[0113] The disclosure of each reference set forth herein is incorporated herein by reference in its entirety.

[0114] The term "NAR" refers to nitrate assimilation related genes. These type of genes and the NAR polypeptides encoded by them are a component of the high affinity nitrate uptake system in plants.

[0115] The term "HAT" is used interchangeably with high affinity nitrate transporter.

[0116] As used herein, an "isolated nucleic acid fragment" is used interchangeably with "isolated polynucleotide" and is a polymer of RNA or DNA that is single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases. An isolated nucleic acid fragment in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA. Nucleotides (usually found in their 5'-monophosphate form) are referred to by their single letter designation as follows: "A" for adenylate or deoxyadenylate (for RNA or DNA, respectively), "C" for cytidylate or deoxycytidylate, "G" for guanylate or deoxyguanylate, "U" for uridylate, "T" for deoxythymidylate, "R" for purines (A or G), "Y" for pyrimidines (C or T), "K" for G or T, "H" for A or C or T, "I" for inosine, and "N" for any nucleotide.

[0117] The term "isolated" refers to materials, such as nucleic acid molecules and/or proteins, which are substantially free or otherwise removed from components that normally accompany or interact with the materials in a naturally occurring environment. Isolated polynucleotides may be purified from a host cell in which they naturally occur. Conventional nucleic acid purification methods known to skilled artisans may be used to obtain isolated polynucleotides. The term also embraces recombinant polynucleotides and chemically synthesized polynucleotides.

[0118] The terms "subfragment that is functionally equivalent" and "functionally equivalent subfragment" are used interchangeably herein. These terms refer to a portion or subsequence of an isolated nucleic acid fragment in which the ability to alter gene expression or produce a certain phenotype is retained whether or not the portion or subsequence encodes an active enzyme or functional protein (for example, the portion or subsequence may be a portion of coding and/or non-coding regions and need not encode an active enzyme or functional protein. For example, the fragment or subfragment can be used in the design of recombinant DNA constructs to produce the desired phenotype in a transformed plant. Recombinant DNA constructs can be designed for use in co-suppression or antisense by linking a nucleic acid fragment or subfragment thereof, whether or not it encodes an active enzyme or functional protein, in the appropriate orientation relative to a plant promoter sequence.

[0119] The terms "homology", "homologous", "substantially similar" and "corresponding substantially" are used interchangeably herein. They refer to nucleic acid fragments wherein changes in one or more nucleotide bases does not affect the ability of the nucleic acid fragment to mediate gene expression or produce a certain phenotype. These terms also refer to modifications of the nucleic acid fragments of the instant invention such as deletion or insertion of one or more nucleotides that do not substantially alter the functional properties of the resulting nucleic acid fragment relative to the initial, unmodified fragment. It is therefore understood, as those skilled in the art will appreciate, that the invention encompasses more than the specific exemplary sequences.

[0120] Moreover, the skilled artisan recognizes that substantially similar nucleic acid sequences encompassed by this invention are also defined by their ability to hybridize, under moderately stringent conditions (for example, 1×SSC, 0.1% SDS, 60° C.) with the sequences exemplified herein, or to any portion of the nucleotide sequences reported herein and which are functionally equivalent to the gene or the promoter of the invention. Stringency conditions can be adjusted to screen for moderately similar fragments, such as homologous sequences from distantly related organisms, to highly similar fragments, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes determine stringency conditions. One set of preferred conditions involves a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A more preferred set of stringent conditions involves the use of higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C. Another preferred set of highly stringent conditions involves the use of two final washes in 0.1×SSC, 0.1% SDS at 65° C.

[0121] With respect to the degree of substantial similarity between the target (endogenous) mRNA and the RNA region in the construct having homology to the target mRNA, such sequences should be at least 25 nucleotides in length, preferably at least 50 nucleotides in length, more preferably at least 100 nucleotides in length, again more preferably at least 200 nucleotides in length, and most preferably at least 300 nucleotides in length; and should be at least 80% identical, preferably at least 85% identical, more preferably at least 90% identical, and most preferably at least 95% identical.

[0122] Substantially similar nucleic acid fragments may be selected by screening nucleic acid fragments representing subfragments or modifications of the nucleic acid fragments of the instant invention, wherein one or more nucleotides are substituted, deleted and/or inserted, for their ability to affect the level of the polypeptide encoded by the unmodified nucleic acid fragment in a plant or plant cell. For example, a substantially similar nucleic acid fragment representing at least 30 contiguous nucleotides, preferably at least 40 contiguous nucleotides, most preferably at least 60 contiguous nucleotides derived from the instant nucleic acid fragment can be constructed and introduced into a plant or plant cell. The level of the polypeptide encoded by the unmodified nucleic acid fragment present in a plant or plant cell exposed to the substantially similar nucleic fragment can then be compared to the level of the polypeptide in a plant or plant cell that is not exposed to the substantially similar nucleic acid fragment.

[0123] Sequence alignments and percent similarity calculations may be determined using a variety of comparison methods designed to detect homologous sequences including, but not limited to, the Megalign program of the LASARGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences are performed using the Clustal V method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal method are KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4.

[0124] "Gene" refers to a nucleic acid fragment that expresses a specific protein, including regulatory sequences preceding (5' non-coding sequences) and following (3' non-coding sequences) the coding sequence. "Native gene" refers to a gene as found in nature with its own regulatory sequences. "Recombinant DNA construct" refers to a combination of nucleic acid fragments that are not normally found together in nature. Accordingly, a recombinant DNA construct may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that normally found in nature. A "foreign" gene refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or recombinant DNA constructs. A "transgene" is a gene that has been introduced into the genome by a transformation procedure.

[0125] "Coding sequence" refers to a DNA sequence that codes for a specific amino acid sequence. "Regulatory sequences" refer to nucleotide sequences located upstream (5' non-coding sequences), within, or downstream (3' non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include, but are not limited to, promoters, translation leader sequences, introns, and polyadenylation recognition sequences.

[0126] "Promoter" refers to a DNA sequence capable of controlling the expression of a coding sequence or functional RNA. The promoter sequence consists of proximal and more distal upstream elements, the latter elements often referred to as enhancers. Accordingly, an "enhancer" is a DNA sequence which can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue-specificity of a promoter. Promoter sequences can also be located within the transcribed portions of genes, and/or downstream of the transcribed sequences. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of an isolated nucleic acid fragment in different tissues or cell types, or at different stages of development, or in response to different environmental conditions. Promoters, which cause an isolated nucleic acid fragment to be expressed in most cell types, at most times are commonly referred to as "constitutive promoters". New promoters of various types useful in plant cells are constantly being discovered; numerous examples may be found in the compilation by Okamuro and Goldberg, (1989) Biochemistry of Plants 15:1-82.

[0127] It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of some variation may have identical promoter activity. As used herein, "substantially similar and functionally equivalent subfragment of a promoter" refers to a portion or subsequence of a promoter sequence which is capable of controlling the expression of a coding sequence or functional RNA.

[0128] Specific examples of promoters that may be useful in expressing the nucleic acid fragments of the invention include, but are not limited to, the promoters disclosed in this application (SEQ ID NOs: δ 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73, 74, 89 or 90).

[0129] An "intron" is an intervening sequence in a gene that does not encode a portion of the protein sequence. Thus, such sequences are transcribed into RNA but are then excised and are not translated. The term is also used for the excised RNA sequences.

[0130] An "exon" is a portion of the sequence of a gene that is transcribed and is found in the mature messenger RNA derived from the gene, but is not necessarily a part of the sequence that encodes the final gene product.

[0131] The term "deduced nucleotide sequence" refers to a DNA sequence after removal of intervening sequences, based on homology to other DNA sequences encoding the same protein.

[0132] The term "deduced amino acid sequence" refers to a polypeptide sequence derived from a DNA sequence after removal of intervening sequences, based on homology to other proteins encoded by DNA sequences encoding the same protein.

[0133] The term "translation leader sequence" refers to a DNA sequence located between the promoter sequence of a gene and the coding sequence. The translation leader sequence is present in the fully processed mRNA upstream of the translation start sequence. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency.

[0134] Examples of translation leader sequences have been described (Turner, R. and Foster, G. D. (1995) Molecular Biotechnology 3:225).

[0135] The "3' non-coding sequences" refer to DNA sequences located downstream of a coding sequence and include polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3' end of the mRNA precursor. The use of different 3' non-coding sequences is exemplified by Ingelbrecht et al., (1989) Plant Cell 1:671-680.

[0136] "RNA transcript" refers to the product resulting from RNA polymerase-catalyzed transcription of a DNA sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred to as the primary transcript or it may be a RNA sequence derived from post-transcriptional processing of the primary transcript and is referred to as the mature RNA. "Messenger RNA (mRNA)" refers to the RNA that is without introns and that can be translated into protein by the cell. "cDNA" refers to a DNA that is complementary to and synthesized from a mRNA template using the enzyme reverse transcriptase. The cDNA can be single-stranded or converted into the double-stranded form using the Klenow fragment of DNA polymerase I. "Sense" RNA refers to RNA transcript that includes the mRNA and can be translated into protein within a cell or in vitro. "Antisense RNA" refers to an RNA transcript that is complementary to all or part of a target primary transcript or mRNA and that blocks the expression of a target isolated nucleic acid fragment (U.S. Pat. No. 5,107,065). The complementarity of an antisense RNA may be with any part of the specific gene transcript, i.e., at the 5' non-coding sequence, 3' non-coding sequence, introns, or the coding sequence. "Functional RNA" refers to antisense RNA, ribozyme RNA, or other RNA that may not be translated but yet has an effect on cellular processes. The terms "complement" and "reverse complement" are used interchangeably herein with respect to mRNA transcripts, and are meant to define the antisense RNA of the message.

[0137] The term "endogenous RNA" refers to any RNA which is encoded by any nucleic acid sequence present in the genome of the host, whether naturally-occurring or non-naturally occurring, i.e., introduced by recombinant means, mutagenesis, etc.

[0138] The term "non-naturally occurring" means artificial, not consistent with what is normally found in nature.

[0139] The term "operably linked" refers to the association of nucleic acid sequences on a single nucleic acid fragment so that the function of one is regulated by the other. For example, a promoter is operably linked with a coding sequence when it is capable of regulating the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in a sense or antisense orientation. In another example, the complementary RNA regions of the invention can be operably linked, either directly or indirectly, 5' to the target mRNA, or 3' to the target mRNA, or within the target mRNA, or a first complementary region is 5' and its complement is 3' to the target mRNA.

[0140] The term "expression", as used herein, refers to the production of a functional end-product. Expression of an isolated nucleic acid fragment involves transcription of the isolated nucleic acid fragment and translation of the mRNA into a precursor or mature protein. "Antisense inhibition" refers to the production of antisense RNA transcripts capable of suppressing the expression of the target protein. "Co-suppression" refers to the production of sense RNA transcripts capable of suppressing the expression of identical or substantially similar foreign or endogenous genes (U.S. Pat. No. 5,231,020).

[0141] "Mature" protein refers to a post-translationally processed polypeptide; i.e., one from which any pre- or propeptides present in the primary translation product have been removed. "Precursor" protein refers to the primary product of translation of mRNA; i.e., with pre- and propeptides still present. Pre- and propeptides may be but are not limited to intracellular localization signals.

[0142] "Stable transformation" refers to the transfer of a nucleic acid fragment into a genome of a host organism, including both nuclear and organellar genomes, resulting in genetically stable inheritance. In contrast, "transient transformation" refers to the transfer of a nucleic acid fragment into the nucleus, or DNA-containing organelle, of a host organism resulting in gene expression without integration or stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as "transgenic" organisms. The preferred method of cell transformation of rice, corn and other monocots is the use of particle-accelerated or "gene gun" transformation technology (Klein et al., (1987) Nature (London) 327:70-73; U.S. Pat. No. 4,945,050), or an Agrobacterium-mediated method using an appropriate Ti plasmid containing the transgene (Ishida Y. et al., 1996, Nature Biotech. 14:745-750). The term "transformation and "transformed" as used herein refer to both stable transformation and transient transformation.

[0143] Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989 (hereinafter "Sambrook").

[0144] The term "recombinant" refers to an artificial combination of two otherwise separated segments of sequence, e.g., by chemical synthesis or by the manipulation of isolated segments of nucleic acids by genetic engineering techniques.

[0145] "PCR" or "Polymerase Chain Reaction" is a technique for the synthesis of large quantities of specific DNA segments, consists of a series of repetitive cycles (Perkin Elmer Cetus Instruments, Norwalk, Conn.). Typically, the double stranded DNA is heat denatured, the two primers complementary to the 3' boundaries of the target segment are annealed at low temperature and then extended at an intermediate temperature. One set of these three consecutive steps is referred to as a cycle.

[0146] Polymerase chain reaction ("PCR") is a powerful technique used to amplify DNA millions of fold, by repeated replication of a template, in a short period of time. (Mullis et al, Cold Spring Harbor Symp. Quant. Biol. 51:263-273 (1986); Erlich et al, European Patent Application 50,424; European Patent Application 84,796; European Patent Application 258,017, European Patent Application 237,362; Mullis, European Patent Application 201,184, Mullis et al U.S. Pat. No. 4,683,202; Erlich, U.S. Pat. No. 4,582,788; and Saiki et al, U.S. Pat. No. 4,683,194). The process utilizes sets of specific in vitro synthesized oligonucleotides to prime DNA synthesis. The design of the primers is dependent upon the sequences of DNA that are desired to be analyzed. The technique is carried out through many cycles (usually 20-50) of melting the template at high temperature, allowing the primers to anneal to complementary sequences within the template and then replicating the template with DNA polymerase.

[0147] The products of PCR reactions are analyzed by separation in agarose gels followed by ethidium bromide staining and visualization with UV transillumination. Alternatively, radioactive dNTPs can be added to the PCR in order to incorporate label into the products. In this case the products of PCR are visualized by exposure of the gel to x-ray film. The added advantage of radiolabeling PCR products is that the levels of individual amplification products can be quantitated.

[0148] The terms "recombinant construct", "expression construct" and "recombinant expression construct" are used interchangeably herein. These terms refer to a functional unit of genetic material that can be inserted into the genome of a cell using standard methodology well known to one skilled in the art. Such construct may be itself or may be used in conjunction with a vector. If a vector is used then the choice of vector is dependent upon the method that will be used to transform host plants as is well known to those skilled in the art. For example, a plasmid vector can be used. The skilled artisan is well aware of the genetic elements that must be present on the vector in order to successfully transform, select and propagate host cells comprising any of the isolated nucleic acid fragments of the invention. The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression (Jones et al., (1985) EMBO J. 4:2411-2418; De Almeida et al., (1989) Mol. Gen. Genetics 218:78-86), and thus that multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Southern analysis of DNA, Northern analysis of mRNA expression, Western analysis of protein expression, or phenotypic analysis.

[0149] Co-suppression constructs in plants previously have been designed by focusing on overexpression of a nucleic acid sequence having homology to an endogenous mRNA, in the sense orientation, which results in the reduction of all RNA having homology to the overexpressed sequence (see Vaucheret et al. (1998) Plant J 16:651-659; and Gura (2000) Nature 404:804-808). The overall efficiency of this phenomenon is low, and the extent of the RNA reduction is widely variable. Recent work has described the use of "hairpin" structures that incorporate all, or part, of an mRNA encoding sequence in a complementary orientation that results in a potential "stem-loop" structure for the expressed RNA (PCT Publication WO 99/53050 published on Oct. 21, 1999). This increases the frequency of co-suppression in the recovered transgenic plants. Another variation describes the use of plant viral sequences to direct the suppression, or "silencing", of proximal mRNA encoding sequences (PCT Publication WO 98/36083 published on Aug. 20, 1998). Both of these co-suppressing phenomena have not been elucidated mechanistically, although recent genetic evidence has begun to unravel this complex situation (Elmayan et al. (1998) Plant Cell 10:1747-1757).

[0150] In one aspect, this invention includes an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide required for high affinity nitrate transport, wherein the polypeptide has an amino acid sequence of at least 80%, 85%, 90%, 95%, or 99% sequence identity, based on the Clustal V method of alignment, when compared to one of SEQ ID NO: 36 or 49. The polypeptide may also comprise SEQ ID NO: 36 or 49, and the nucleotide sequence may comprise SEQ ID NO: 35 or 48.

[0151] Also included in the present invention is a complement of any of the foregoing nucleotide sequences, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.

[0152] In another aspect, this invention includes isolated polynucleotides as described herein (or complements), wherein the nucleotide sequence comprises at least two, three, four, or five motifs selected from group consisting of SEQ ID NOs: 50, 51 and 52, wherein said motif is a substantially conserved subsequence.

[0153] "Motifs" or "subsequences" refer to short regions of conserved sequences of nucleic acids or amino acids that comprise part of a longer sequence. For example, it is expected that such conserved subsequences (for example SEQ ID NOs: 50, 51 and 52) would be important for function, and could be used to identify new homologues of high affinity nitrate transporter-homologues in plants. It is expected that some or all of the elements may be found in a high affinity nitrate transporter-homologue. Also, it is expected that at least one or two of the conserved amino acids in any given motif may differ in a true high affinity nitrate transporter-homologue.

[0154] In another aspect, a polynucleotide of this invention or a functionally equivalent subfragment thereof is useful in antisense inhibition or cosuppression of expression of nucleic acid sequences encoding proteins required for high affinity nitrate transport, most preferably in antisense inhibition or cosuppression of an endogenous high affinity nitrate transporter or heterologous high affinity nitrate transporter gene.

[0155] Protocols for antisense inhibition or co-suppression are well known to those skilled in the art and are described above.

[0156] In still a further aspect, this invention includes an isolated nucleic acid fragment comprising (a) a promoter consisting essentially of SEQ ID NO: δ 37, 38, 46, 47, 56, 65, 67, 68, 69, 70, 71, 72, 73, 74, 89 or 90 or (b) a substantially similar and functionally equivalent subfragment of said promoter.

[0157] Also of interest are recombinant DNA constructs comprising any of the above-identified isolated nucleic acid fragments or isolated polynucleotides or complements thereof or parts of such fragments or complements, operably linked to at least one regulatory sequence.

[0158] Plants, plant tissue or plant cells comprising such recombinant DNA constructs in their genome are also within the scope of this invention. Transformation methods are well known to those skilled in the art and are described above. Any plant, dicot or monocot can be transformed with such recombinant DNA constructs.

[0159] Examples of monocots include, but are not limited to, corn, wheat, rice, sorghum, millet, barley, palm, lily, Alstroemeria, rye, and oat. Examples of dicots include, but are not limited to, soybean, rape, sunflower, canola, grape, guayule, columbine, cotton, tobacco, peas, beans, flax, safflower, alfalfa.

[0160] Plant tissue includes differentiated and undifferentiated tissues or plants, including but not limited to, roots, stems, shoots, leaves, pollen, seeds, tumor tissue, and various forms of cells and culture such as single cells, protoplasm, embryos, and callus tissue. The plant tissue may be in plant or in organ, tissue or cell culture.

[0161] In another aspect, this invention includes a method of altering plant nitrate transport, comprising:

[0162] (a) transforming a plant with a recombinant DNA construct comprising:

[0163] i) A recombinant DNA construct comprising an isolated polynucleotide encoding a HAT polypeptide, operably linked to at least one regulatory sequence; and

[0164] ii) at least one additional recombinant DNA construct comprising an isolated polynucleotide encoding a NAR polypeptide, operably linked to at least one regulatory sequence.

[0165] (b) growing the transformed plant of (a) under conditions suitable for the expression of the recombinant DNA construct; and selecting those transformed plants having altered nitrate transport.

[0166] As used herein, altering plant nitrate transport may result in increased or decreased changes.

[0167] The regeneration, development, and cultivation of plants from single plant protoplast transformants or from various transformed explants is well known in the art (Weissbach and Weissbach, In: Methods for Plant Molecular Biology, (Eds.), Academic Press, Inc. San Diego, Calif., (1988)). This regeneration and growth process typically includes the steps of selection of transformed cells, culturing those individualized cells through the usual stages of embryonic development through the rooted plantlet stage. Transgenic embryos and seeds are similarly regenerated. The resulting transgenic rooted shoots are thereafter planted in an appropriate plant growth medium such as soil.

[0168] The development or regeneration of plants containing the foreign, exogenous isolated nucleic acid fragment that encodes a protein of interest is well known in the art. Preferably, the regenerated plants are self-pollinated to provide homozygous transgenic plants. Otherwise, pollen obtained from the regenerated plants is crossed to seed-grown plants of agronomically important lines. Conversely, pollen from plants of these important lines is used to pollinate regenerated plants. A transgenic plant of the present invention containing a desired polypeptide is cultivated using methods well known to one skilled in the art.

[0169] There are a variety of methods for the regeneration of plants from plant tissue.

[0170] The particular method of regeneration will depend on the starting plant tissue and the particular plant species to be regenerated.

[0171] Methods for transforming dicots, primarily by use of Agrobacterium tumefaciens, and obtaining transgenic plants have been published for cotton (U.S. Pat. No. 5,004,863, U.S. Pat. No. 5,159,135, U.S. Pat. No. 5,518,908); soybean (U.S. Pat. No. 5,569,834, U.S. Pat. No. 5,416,011, McCabe et. al., Bio/Technology 6:923 (1988), Christou et al., Plant Physiol. 87:671-674 (1988)); Brassica (U.S. Pat. No. 5,463,174); peanut (Cheng et al., Plant Cell Rep. 15:653-657 (1996), McKently et al., Plant Cell Rep. 14:699-703 (1995)); papaya; and pea (Grant et al., Plant Cell Rep. 15:254-258, (1995)).

[0172] Transformation of monocotyledons using electroporation, particle bombardment, and Agrobacterium have also been reported. Transformation and plant regeneration have been achieved in asparagus (Bytebier et al., Proc. Natl. Acad. Sci. (USA) 84:5354, (1987)); barley (Wan and Lemaux, Plant Physiol 104:37 (1994)); Zea mays (Rhodes et al., Science 240:204 (1988), Gordon-Kamm et al., Plant Cell 2:603-618 (1990), Fromm et al., Bio/Technology 8:833 (1990), Koziel et al., Bio/Technology 11: 194, (1993), Armstrong et al., Crop Science 35:550-557 (1995)); oat (Somers et al., Bio/Technology 10: 15 89 (1992)); orchard grass (Horn et al., Plant Cell Rep. 7:469 (1988)); rice (Toriyama et al., TheorAppl. Genet. 205:34, (1986); Part et al., Plant Mol. Biol. 32:1135-1148, (1996); Abedinia et al., Aust. J. Plant Physiol. 24:133-141 (1997); Zhang and Wu, Theor. Appl. Genet. 76:835 (1988); Zhang et al. Plant Cell Rep. 7:379, (1988); Battraw and Hall, Plant Sci. 86:191-202 (1992); Christou et al., Bio/Technology 9:957 (1991)); rye (De la Pena et al., Nature 325:274 (1987)); sugarcane (Bower and Birch, Plant J. 2:409 (1992)); tall fescue (Wang et al., Bio/Technology 10:691 (1992)), and wheat (Vasil et al., Bio/Technology 10:667 (1992); U.S. Pat. No. 5,631,152).

[0173] Assays for gene expression based on the transient expression of cloned nucleic acid constructs have been developed by introducing the nucleic acid molecules into plant cells by polyethylene glycol treatment, electroporation, or particle bombardment (Marcotte et al., Nature 335:454-457 (1988); Marcotte et al., Plant Cell 1:523-532 (1989); McCarty et al., Cell 66:895-905 (1991); Hattori et al., Genes Dev. 6:609-618 (1992); Goff et al., EMBO J. 9:2517-2522 (1990)).

[0174] Transient expression systems may be used to functionally dissect isolated nucleic acid fragment constructs (see generally, Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Press (1995)). It is understood that any of the nucleic acid molecules of the present invention can be introduced into a plant cell in a permanent or transient manner in combination with other genetic elements such as vectors, promoters, enhancers etc.

[0175] In addition to the above discussed procedures, practitioners are familiar with the standard resource materials which describe specific conditions and procedures for the construction, manipulation and isolation of macromolecules (e.g., DNA molecules, plasmids, etc.), generation of recombinant organisms and the screening and isolating of clones, (see for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press (1989); Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Press (1995); Birren et al., Genome Analysis: Detecting Genes, 1, Cold Spring Harbor, N.Y. (1998); Birren et al., Genome Analysis Analyzing DNA, 2, Cold Spring Harbor, N.Y. (1998); Plant Molecular Biology: A Laboratory Manual, eds. Clark, Springer, N.Y. (1997)).

[0176] In a still further aspect, this invention includes a method to isolate nucleic acid fragments encoding polypeptides associated with altering plant nitrate transport, which comprises:

[0177] (a) comparing SEQ ID NO: 36 or 49 with other polypeptide sequences associated with altering plant nitrate transport;

[0178] (b) identifying conserved sequences of 4 or more amino acids obtained in step (a);

[0179] (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b); and

[0180] (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering plant nitrate transport by sequence dependent protocols.

[0181] Examples of conserved sequence elements that would be useful in identifying other plant sequences associated with altering plant nitrate transport can be found in the group comprising, but not limited to, the nucleotides encoding the polypeptides of SEQ ID NOs: 50, 51, and 52.

[0182] In another aspect, this invention also includes a method of mapping genetic variations related to altering plant nitrate transport comprising:

[0183] (a) crossing two plant varieties; and

[0184] (b) evaluating genetic variations with respect to:

[0185] (i) a nucleic acid sequence selected from the group consisting of SEQ ID NO: 35 and 48; or

[0186] (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs: 36 and 49 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.

[0187] In another embodiment, this invention includes a method of molecular breeding to obtain altered plant nitrate transport:

[0188] (a) crossing two plant varieties; and

[0189] (b) evaluating genetic variations with respect to:

[0190] (i) a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 35 and 48; or

[0191] (ii) a nucleic acid sequence encoding a polypeptide selected from the group consisting of SEQ ID NOs: 36 and 49 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.

[0192] The terms "mapping genetic variation" or "mapping genetic variability" are used interchangeably and define the process of identifying changes in DNA sequence, whether from natural or induced causes, within a genetic region that differentiates between different plant lines, cultivars, varieties, families, or species. The genetic variability at a particular locus (gene) due to even minor base changes can alter the pattern of restriction enzyme digestion fragments that can be generated. Pathogenic alterations to the genotype can be due to deletions or insertions within the gene being analyzed or even single nucleotide substitutions that can create or delete a restriction enzyme recognition site. RFLP (restriction fragment length polymorphisms) analysis takes advantage of this and utilizes Southern blotting with a probe corresponding to the isolated nucleic acid fragment of interest.

[0193] Thus, if a polymorphism (i.e., a commonly occurring variation in a gene or segment of DNA; also, the existence of several forms of a gene (alleles) in the same species) creates or destroys a restriction endonuclease cleavage site, or if it results in the loss or insertion of DNA (e.g., a variable nucleotide tandem repeat (VNTR) polymorphism), it will alter the size or profile of the DNA fragments that are generated by digestion with that restriction endonuclease. As such, individuals that possess a variant sequence can be distinguished from those having the original sequence by restriction fragment analysis. Polymorphisms that can be identified in this manner are termed RFLPs. RFLPs have been widely used in human and plant genetic analyses (Glassberg, UK Patent Application 2135774; Skolnick et al, Cytogen. Cell Genet. 32:58-67 (1982); Botstein et al, Ann. J. Hum. Genet. 32:314-331 (1980); Fischer et al (PCT Application WO 90/13668; Uhlen, PCT Application WO 90/11369).

[0194] A central attribute of "single nucleotide polymorphisms" or "SNPs" is that the site of the polymorphism is at a single nucleotide. SNPs have certain reported advantages over RFLPs or VNTRs. First, SNPs are more stable than other classes of polymorphisms. Their spontaneous mutation rate is approximately 10^-9 (Kornberg, DNA Replication, W.H. Freeman & Co., San Francisco, 1980), approximately, 1,000 times less frequent than VNTRs (U.S. Pat. No. 5,679,524). Second, SNPs occur at greater frequency, and with greater uniformity than RFLPs and VNTRs. As SNPs result from sequence variation, sequencing random genomic or cDNA molecules can identify new polymorphisms. SNPs can also result from deletions, point mutations and insertions. Any single base alteration, whatever the cause, can be a SNP. The greater frequency of SNPs means that they can be more readily identified than the other classes of polymorphisms.

[0195] SNPs can be characterized using any of a variety of methods. Such methods include the direct or indirect sequencing of the site, the use of restriction enzymes where the respective alleles of the site create or destroy a restriction site, the use of allele-specific hybridization probes, the use of antibodies that are specific for the proteins encoded by the different alleles of the polymorphism or by other biochemical interpretation. SNPs can be sequenced by a number of methods. Two basic methods may be used for DNA sequencing, the chain termination method of Sanger et al, Proc. Natl. Acad. Sci. (U.S.A.) 74:5463-5467 (1977), and the chemical degradation method of Maxam and Gilbert, Proc. Natl. Acad. Sci. (U.S.A.) 74: 560-564 (1977).

[0196] Furthermore, single point mutations can be detected by modified PCR techniques such as the ligase chain reaction ("LCR") and PCR-single strand conformational polymorphisms ("PCR-SSCP") analysis. The PCR technique can also be used to identify the level of expression of genes in extremely small samples of material, e.g., tissues or cells from a body. The technique is termed reverse transcription-PCR ("RT-PCR").

[0197] The term "molecular breeding" defines the process of tracking molecular markers during the breeding process. It is common for the molecular markers to be linked to phenotypic traits that are desirable. By following the segregation of the molecular marker or genetic trait, instead of scoring for a phenotype, the breeding process can be accelerated by growing fewer plants and eliminating assaying or visual inspection for phenotypic variation. The molecular markers useful in this process include, but are not limited to, any marker useful in identifying mapable genetic variations previously mentioned, as well as any closely linked genes that display synteny across plant species. The term "synteny" refers to the conservation of gene placement/order on chromosomes between different organisms. This means that two or more genetic loci, that may or may not be closely linked, are found on the same chromosome among different species. Another term for synteny is "genome colinearity".

[0198] The nucleic acid fragments of the instant invention may be used to create transgenic plants in which the disclosed polypeptides are present at higher or lower levels than normal or in cell types or developmental stages in which they are not normally found. This would have the effect of altering the level of nitrogen transport and accumulation in those cells. Nitrogen deficiency in plants results in stunted growth, and many times in slender and often woody stems. In many plants the first signal of nitrogen deficiency is chlorosis (yellowing of the leaves).

[0199] Overexpression of the proteins of the instant invention may be accomplished by first making a recombinant DNA construct in which the coding region is operably linked to a promoter capable of directing expression of a gene in the desired tissues at the desired stage of development. For reasons of convenience, the recombinant DNA construct may comprise promoter sequences and translation leader sequences derived from the same genes. 3' Non-coding sequences encoding transcription termination signals may also be provided. The instant recombinant DNA construct may also comprise one or more introns in order to facilitate gene expression.

[0200] Plasmid vectors comprising the instant recombinant DNA construct can then be made. The choice of plasmid vector is dependent upon the method that will be used to transform host plants. The skilled artisan is well aware of the genetic elements that must be present on the plasmid vector in order to successfully transform, select and propagate host cells containing the recombinant DNA construct. The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression (Jones et al. (1985) EMBO J. 4:2411-2418; De Almeida et al. (1989) Mol. Gen. Genetics 218:78-86), and thus that multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Southern analysis of DNA, Northern analysis of mRNA expression, Western analysis of protein expression, or phenotypic analysis.

[0201] For some applications it may be useful to direct the instant polypeptides to different cellular compartments, or to facilitate its secretion from the cell. It is thus envisioned that the recombinant DNA construct described above may be further supplemented by altering the coding sequence to encode the instant polypeptides with appropriate intracellular targeting sequences such as transit sequences (Keegstra (1989) Cell 56:247-253), signal sequences or sequences encoding endoplasmic reticulum localization (Chrispeels (1991) Ann. Rev. Plant Phys. Plant Mol. Biol. 42:21-53), or nuclear localization signals (Raikhel (1992) Plant Phys. 100:1627-1632) added and/or with targeting sequences that are already present removed. While the references cited give examples of each of these, the list is not exhaustive and more targeting signals of utility may be discovered in the future.

[0202] It may also be desirable to reduce or eliminate expression of genes encoding the instant polypeptides in plants for some applications. In order to accomplish this, a recombinant DNA construct designed for co-suppression of the instant polypeptide can be constructed by linking a gene or gene fragment encoding that polypeptide to plant promoter sequences. Alternatively, a recombinant DNA construct designed to express antisense RNA for all or part of the instant nucleic acid fragment can be constructed by linking the gene or gene fragment in reverse orientation to plant promoter sequences. Either the co-suppression or antisense recombinant DNA constructs could be introduced into plants via transformation wherein expression of the corresponding endogenous genes are reduced or eliminated.

[0203] Molecular genetic solutions to the generation of plants with altered gene expression have a decided advantage over more traditional plant breeding approaches. Changes in plant phenotypes can be produced by specifically inhibiting expression of one or more genes by antisense inhibition or cosuppression (U.S. Pat. Nos. 5,190,931, 5,107,065 and 5,283,323). An antisense or cosuppression construct would act as a dominant negative regulator of gene activity. While conventional mutations can yield negative regulation of gene activity these effects are most likely recessive. The dominant negative regulation available with a transgenic approach may be advantageous from a breeding perspective. In addition, the ability to restrict the expression of specific phenotype to the reproductive tissues of the plant by the use of tissue specific promoters may confer agronomic advantages relative to conventional mutations which may have an effect in all tissues in which a mutant gene is ordinarily expressed.

[0204] The person skilled in the art will know that special considerations are associated with the use of antisense or cosuppression technologies in order to reduce expression of particular genes. For example, the proper level of expression of sense or antisense genes may require the use of different recombinant DNA constructs utilizing different regulatory elements known to the skilled artisan. Once transgenic plants are obtained by one of the methods described above, it will be necessary to screen individual transgenics for those that most effectively display the desired phenotype. Accordingly, the skilled artisan will develop methods for screening large numbers of transformants. The nature of these screens will generally be chosen on practical grounds, and is not an inherent part of the invention. For example, one can screen by looking for changes in gene expression by using antibodies specific for the protein encoded by the gene being suppressed, or one could establish assays that specifically measure enzyme activity. A preferred method will be one which allows large numbers of samples to be processed rapidly, since it will be expected that a large number of transformants will be negative for the desired phenotype.

[0205] The instant polypeptides (or portions thereof) may be produced in heterologous host cells, particularly in the cells of microbial hosts, and can be used to prepare antibodies to these proteins by methods well known to those skilled in the art. The antibodies are useful for detecting the polypeptides of the instant invention in situ in cells or in vitro in cell extracts. Preferred heterologous host cells for production of the instant polypeptides are microbial hosts. Microbial expression systems and expression vectors containing regulatory sequences that direct high level expression of foreign proteins are well known to those skilled in the art. Any of these could be used to construct a recombinant DNA construct for production of the instant polypeptides. This recombinant DNA construct could then be introduced into appropriate microorganisms via transformation to provide high level expression of the encoded ammonium transporter. An example of a vector for high level expression of the instant polypeptides in a bacterial host is provided (Example 7).

[0206] Additionally, the instant polypeptides can be used as targets to facilitate design and/or identification of inhibitors of those enzymes that may be useful as herbicides. This is desirable because the polypeptides described herein catalyze various steps in nitrogen uptake. Accordingly, inhibition of the activity of one or more of the enzymes described herein could lead to inhibition of plant growth. Thus, the instant polypeptides could be appropriate for new herbicide discovery and design.

[0207] All or a substantial portion of the nucleic acid fragments of the instant invention may also be used as probes for genetically and physically mapping the genes that they are a part of, and as markers for traits linked to those genes. Such information may be useful in plant breeding in order to develop lines with desired phenotypes. For example, the instant nucleic acid fragments may be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Maniatis) of restriction-digested plant genomic DNA may be probed with the nucleic acid fragments of the instant invention. The resulting banding patterns may then be subjected to genetic analyses using computer programs such as MapMaker (Lander et al. (1987) Genomics 1:174-181) in order to construct a genetic map. In addition, the nucleic acid fragments of the instant invention may be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs of a set of individuals representing parent and progeny of a defined genetic cross. Segregation of the DNA polymorphisms is noted and used to calculate the position of the instant nucleic acid sequence in the genetic map previously obtained using this population (Botstein et al. (1980) Am. J. Hum. Genet. 32:314-331).

[0208] The production and use of plant gene-derived probes for use in genetic mapping is described in Bernatzky and Tanksley (1986) Plant Mol. Biol. Reporter 4(1):37-41. Numerous publications describe genetic mapping of specific cDNA clones using the methodology outlined above or variations thereof. For example, F2 intercross populations, backcross populations, randomly mated populations, near isogenic lines, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

[0209] Nucleic acid probes derived from the instant nucleic acid sequences may also be used for physical mapping (i.e., placement of sequences on physical maps; see Hoheisel et al. In: Nonmammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited therein).

[0210] In another embodiment, nucleic acid probes derived from the instant nucleic acid sequences may be used in direct fluorescence in situ hybridization (FISH) mapping (Trask (1991) Trends Genet. 7:149-154). Although current methods of FISH mapping favor use of large clones (several to several hundred KB; see Laan et al. (1995) Genome Research 5:13-20), improvements in sensitivity may allow performance of FISH mapping using shorter probes.

[0211] A variety of nucleic acid amplification-based methods of genetic and physical mapping may be carried out using the instant nucleic acid sequences. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med. 11:95-96), polymorphism of PCR-amplified fragments (CAPS; Sheffield et al. (1993) Genomics 16:325-332), allele-specific ligation (Landegren et al. (1988) Science 241:1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res. 18:3671), Radiation Hybrid Mapping (Walter et al. (1997) Nature Genetics 7:22-28) and Happy Mapping (Dear and Cook (1989) Nucleic Acid Res. 17:6795-6807). For these methods, the sequence of a nucleic acid fragment is used to design and produce primer pairs for use in the amplification reaction or in primer extension reactions. The design of such primers is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the parents of the mapping cross in the region corresponding to the instant nucleic acid sequence. This, however, is generally not necessary for mapping methods.

[0212] Loss of function mutant phenotypes may be identified for the instant cDNA clones either by targeted gene disruption protocols or by identifying specific mutants for these genes contained in a maize population carrying mutations in all possible genes (Ballinger and Benzer (1989) Proc. Natl. Acad. Sci. USA 86:9402-9406; Koes et al. (1995) Proc. Natl. Acad. Sci. USA 92:8149-8153; Bensen et al. (1995) Plant Cell 7:75-84). The latter approach may be accomplished in two ways. First, short segments of the instant nucleic acid fragments may be used in polymerase chain reaction protocols in conjunction with a mutation tag sequence primer on DNAs prepared from a population of plants in which Mutator transposons or some other mutation-causing DNA element has been introduced (see Bensen, supra). The amplification of a specific DNA fragment with these primers indicates the insertion of the mutation tag element in or near the plant gene encoding the instant polypeptides. Alternatively, the instant nucleic acid fragment may be used as a hybridization probe against PCR amplification products generated from the mutation population using the mutation tag sequence primer in conjunction with an arbitrary genomic site primer, such as that for a restriction enzyme site-anchored synthetic adaptor. With either method, a plant containing a mutation in the endogenous gene encoding the instant polypeptides can be identified and obtained. This mutant plant can then be used to determine or confirm the natural function of the instant polypeptides disclosed herein.

[0213] The function of the high affinity nitrate transporters and polypeptides required for high affinity nitrate transport can be confirmed using the TUSC Mutant population. The Trait Utility System for Corn (TUSC) is a method that employs genetic and molecular techniques to facilitate the study of gene function in maize. Studying gene function implies that the gene's sequence is already known, thus the method works in reverse: from sequence to phenotype. This kind of application is referred to as "reverse genetics", which contrasts with "forward" methods (such as transposon tagging) that are designed to identify and isolate the gene(s) responsible for a particular trait (phenotype).

[0214] Pioneer Hi-Bred International, Inc., has its proprietary collection of maize genomic DNA from approximately 42,000 individual F₁ plants (Reverse genetics for maize; Meeley, R and Briggs, S, 1995, Maize Genet. Coop. Newslett. 69:67, 82).

[0215] The genome of each of these individuals contains multiple copies of the transposable element family, Mutator (Mu). The Mu family is highly mutagenic; in the presence of the active element Mu-DR, these elements transpose throughout the genome, inserting into genic regions, and often disrupting gene function. By collecting genomic DNA from a large number of individuals (42,000), Pioneer has assembled a library of the mutagenized maize genome. Mu insertion events are predominately heterozygous so; given the recessive nature of most insertional mutations, the F₁ plants appear wild-type. Each of the plants was selfed to produce F₂ seed, which was collected. In generating the F₂ progeny, insertional mutations segregate in a Mendelian fashion and therefore are useful for investigating a mutant allele's effect on the phenotype. The TUSC system has been successfully used by a number of laboratories to identify the function of a variety of genes (Cloning and characterization of the maize An1 gene, Bensen, R J et al., 1995, Plant Cell 7:75-84; Diversification of C-function activity in maize flower development, Mena, M et al., 1996, Science 274:1537-1540; Analysis of a chemical plant defense mechanism in grasses, Frey, Metal., 1997, Science 277:696-699; The control of maize spikelet meristem fate by the APETALA2-like gene Indeterminate spikelet 1, Chuck, G, Meeley, R B, and Hake, S, 1998, Genes & Development 12:1145-1154; A SecY homologue is required for the elaboration of the chloroplast thylakoid membrane and for normal chloroplast gene expression, Roy, L M and Barkan, A., 1998, J. Cell Biol. 141:1-11).

[0216] Polynucleotide sequences produced by diversity generation methods or recursive sequence recombination ("RSR") methods (e.g., DNA shuffling) are a feature of the invention. Mutation and recombination methods using the nucleic acids described herein are a feature of the invention. For example, one method of the invention includes recursively recombining one or more nucleotide sequences of the invention as described above and below with one or more additional nucleotides. The recombining steps are optionally performed in vivo, ex vivo, in silico or in vitro. This diversity generation or recursive sequence recombination produces at least one library of recombinant modified HAT polynucleotides. Polypeptides encoded by members of this library are included in the invention.

[0217] Descriptions of a variety of diversity generating procedures, including multigene shuffling and methods for generating modified nucleic acid sequences encoding multiple enzymatic domains, are found the following publications and the references cited therein: Soong, N. et al. (2000) "Molecular breeding of viruses" Nat Genet. 25(4):436-39; Stemmer, et al. (1999) "Molecular breeding of viruses for targeting and other clinical properties" Tumor Targeting 4:1-4; Ness et al. (1999) "DNA Shuffling of subgenomic sequences of subtilisin" Nature Biotechnology 17:893-896; Chang et al. (1999) "Evolution of a cytokine using DNA family shuffling" Nature Biotechnology 17:793-797; Minshull and Stemmer (1999) "Protein evolution by molecular breeding" Current Opinion in Chemical Biology 3:284-290; Christians et al. (1999) "Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling" Nature Biotechnology 17:259-264; Crameri et al. (1998) "DNA shuffling of a family of genes from diverse species accelerates directed evolution" Nature 391:288-291; Crameri et al. (1997) "Molecular evolution of an arsenate detoxification pathway by DNA shuffling," Nature Biotechnology 15:436-438; Zhang et al. (1997) "Directed evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening" Proc. Natl. Acad. Sci. USA 94:4504-4509; Patten et al. (1997) "Applications of DNA Shuffling to Pharmaceuticals and Vaccines" Current Opinion in Biotechnology 8:724-733; Crameri et al. (1996) "Construction and evolution of antibody-phage libraries by DNA shuffling" Nature Medicine 2:100-103; Crameri et al. (1996) "Improved green fluorescent protein by molecular evolution using DNA shuffling" Nature Biotechnology 14:315-319; Gates et al. (1996) "Affinity selective isolation of ligands from peptide libraries through display on a lac repressor `headpiece dimer`" Journal of Molecular Biology 255:373-386; Stemmer (1996) "Sexual PCR and Assembly PCR" In: The Encyclopedia of Molecular Biology. VCH Publishers, New York. pp. 447-457; Crameri and Stemmer (1995) "Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wildtype cassettes" BioTechniques 18:194-195; Stemmer et al., (1995) "Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxy-ribonucleotides" Gene, 164:49-53; Stemmer (1995) "The Evolution of Molecular Computation" Science 270: 1510; Stemmer (1995) "Searching Sequence Space" Bio/Technology 13:549-553; Stemmer (1994) "Rapid evolution of a protein in vitro by DNA shuffling" Nature 370:389-391; and Stemmer (1994) "DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution." Proc. Natl. Acad. Sci. USA 91:10747-10751. Additional details regarding various diversity generating methods can be found in the following U.S. patents, PCT publications, and EPO publications: U.S. Pat. No. 5,605,793 to Stemmer (Feb. 25, 1997), "Methods for In Vitro Recombination;" U.S. Pat. No. 5,811,238 to Stemmer et al. (Sep. 22, 1998) "Methods for Generating Polynucleotides having Desired Characteristics by Iterative Selection and Recombination;" U.S. Pat. No. 5,830,721 to Stemmer et al. (Nov. 3, 1998), "DNA Mutagenesis by Random Fragmentation and Reassembly;" U.S. Pat. No. 5,834,252 to Stemmer, et al. (Nov. 10, 1998) "End-Complementary Polymerase Reaction;" U.S. Pat. No. 5,837,458 to Minshull, et al. (Nov. 17, 1998), "Methods and Compositions for Cellular and Metabolic Engineering;" WO 95/22625, Stemmer and Crameri, "Mutagenesis by Random Fragmentation and Reassembly;" WO 96/33207 by Stemmer and Lipschutz "End Complementary Polymerase Chain Reaction;" WO 97/20078 by Stemmer and Crameri "Methods for Generating Polynucleotides having Desired Characteristics by Iterative Selection and Recombination;" WO 97/35966 by Minshull and Stemmer, "Methods and Compositions for Cellular and Metabolic Engineering;" WO 99/41402 by Punnonen et al. "Targeting of Genetic Vaccine Vectors;" WO 99/41383 by Punnonen et al. "Antigen Library Immunization;" WO 99/41369 by Punnonen et al. "Genetic Vaccine Vector Engineering;" WO 99/41368 by Punnonen et al. "Optimization of Immunomodulatory Properties of Genetic Vaccines;" EP 752008 by Stemmer and Crameri, "DNA Mutagenesis by Random Fragmentation and Reassembly;" EP 0932670 by Stemmer "Evolving Cellular DNA Uptake by Recursive Sequence Recombination;" WO 99/23107 by Stemmer et al., "Modification of Virus Tropism and Host Range by Viral Genome Shuffling;" WO 99/21979 by Apt et al., "Human Papillomavirus Vectors;" WO 98/31837 by del Cardayre et al. "Evolution of Whole Cells and Organisms by Recursive Sequence Recombination;" WO 98/27230 by Patten and Stemmer, "Methods and Compositions for Polypeptide Engineering;" WO 98/13487 by Stemmer et al., "Methods for Optimization of Gene Therapy by Recursive Sequence Shuffling and Selection;" WO 00/00632, "Methods for Generating Highly Diverse Libraries;" WO 00/09679, "Methods for Obtaining in Vitro Recombined Polynucleotide Sequence Banks and Resulting Sequences;" WO 98/42832 by Arnold et al., "Recombination of Polynucleotide Sequences Using Random or Defined Primers;" WO 99/29902 by Arnold et al., "Method for Creating Polynucleotide and Polypeptide Sequences;" WO 98/41653 by Vind, "An in Vitro Method for Construction of a DNA Library;" WO 98/41622 by Borchert et al., "Method for Constructing a Library Using DNA Shuffling;" WO 98/42727 by Pati and Zarling, "Sequence Alterations using Homologous Recombination;" WO00/18906 by Patten et al., "Shuffling of Codon-Altered Genes;" WO 00/04190 by del Cardayre et al. "Evolution of Whole Cells and Organisms by Recursive Recombination;" WO 00/42561 by Crameri et al., "Oligonucleotide Mediated Nucleic Acid Recombination;" WO 00/42559 by Selifonov and Stemmer "Methods of Populating Data Structures for Use in Evolutionary Simulations;" WO 00/42560 by Selifonov et al., "Methods for Making Character Strings, Polynucleotides & Polypeptides Having Desired Characteristics;" WO 01/23401 by Welch et al., "Use of Codon-Varied Oligonucleotide Synthesis for Synthetic Shuffling;" and WO 01/64864 "Single-Stranded Nucleic Acid Template-Mediated Recombination and Nucleic Acid Fragment Isolation" by Affholter.

[0218] Certain U.S. applications provide additional details regarding various diversity generating methods, including "SHUFFLING OF CODON ALTERED GENES" by Patten et al. filed Sep. 28, 1999, (U.S. Ser. No. 09/407,800); "EVOLUTION OF WHOLE CELLS AND ORGANISMS BY RECURSIVE SEQUENCE RECOMBINATION", by del Cardayre et al. filed Jul. 15, 1998 (U.S. Ser. No. 09/166,188), and Jul. 15, 1999 (U.S. Pat. No. 6,379,964); "OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION" by Crameri et al., filed Sep. 28, 1999 (U.S. Pat. No. 6,376,246); "OLIGONUCLEOTIDE MEDIATED NUCLEIC ACID RECOMBINATION" by Crameri et al., filed Jan. 18, 2000 (WO 00/42561); "USE OF CODON-BASED OLIGONUCLEOTIDE SYNTHESIS FOR SYNTHETIC SHUFFLING" by Welch et al., filed Sep. 28, 1999 (U.S. Pat. No. 6,436,675); "METHODS FOR MAKING CHARACTER STRINGS, POLYNUCLEOTIDES & POLYPEPTIDES HAVING DESIRED CHARACTERISTICS" by Selifonov et al., filed Jan. 18, 2000, (WO 00/42560); "METHODS FOR MAKING CHARACTER STRINGS, POLYNUCLEOTIDES & POLYPEPTIDES HAVING DESIRED CHARACTERISTICS" by Selifonov et al., filed Jul. 18, 2000 (U.S. Ser. No. 09/618,579); "METHODS OF POPULATING DATA STRUCTURES FOR USE IN EVOLUTIONARY SIMULATIONS" by Selifonov and Stemmer (WO 00/42559), filed Jan. 18, 2000; and "SINGLE-STRANDED NUCLEIC ACID TEMPLATE-MEDIATED RECOMBINATION AND NUCLEIC ACID FRAGMENT ISOLATION" by Affholter (U.S. Ser. No. 60/186,482, filed Mar. 2, 2000). Synthetic recombination methods can also be used, in which oligonucleotides corresponding to targets of interest are synthesized and reassembled in PCR or ligation reactions which include oligonucleotides which correspond to more than one parental nucleic acid, thereby generating new recombined nucleic acids. Oligonucleotides can be made by standard nucleotide addition methods, or can be made, e.g., by tri-nucleotide synthetic approaches. Details regarding such approaches are found in the references noted above, including, e.g., WO 00/42561 by Crameri et al., "Oligonucleotide Mediated Nucleic Acid Recombination;" WO 01/23401 by Welch et al., "Use of Codon-Varied Oligonucleotide Synthesis for Synthetic Shuffling;" WO 00/42560 by Selifonov et al., "Methods for Making Character Strings, Polynucleotides and Polypeptides Having Desired Characteristics;" and WO 00/42559 by Selifonov and Stemmer "Methods of Populating Data Structures for Use in Evolutionary Simulations."

[0219] In silico methods of recombination can be effected in which genetic algorithms are used in a computer to recombine sequence strings which correspond to homologous (or even non-homologous) nucleic acids. The resulting recombined sequence strings are optionally converted into nucleic acids by synthesis of nucleic acids, which correspond to the recombined sequences, e.g., in concert with oligonucleotide synthesis gene reassembly techniques. This approach can generate random, partially random or designed variants. Many details regarding in silico recombination, including the use of genetic algorithms, genetic operators and the like in computer systems, combined with generation of corresponding nucleic acids (and/or proteins), as well as combinations of designed nucleic acids and/or proteins (e.g., based on cross-over site selection) as well as designed, pseudo-random or random recombination methods are described in WO 00/42560 by Selifonov et al., "Methods for Making Character Strings, Polynucleotides and Polypeptides Having Desired Characteristics" and WO 00/42559 by Selifonov and Stemmer "Methods of Populating Data Structures for Use in Evolutionary Simulations." Extensive details regarding in silico recombination methods are found in these applications. This methodology is generally applicable to the present invention in providing for recombination of nucleic acid sequences and/or gene fusion constructs encoding proteins involved in various metabolic pathways (such as, for example, carotenoid biosynthetic pathways, ectoine biosynthetic pathways, polyhydroxyalkanoate biosynthetic pathways, aromatic polyketide biosynthetic pathways, and the like) in silico and/or the generation of corresponding nucleic acids or proteins.

[0220] Many of the above-described methodologies for generating modified polynucleotides generate a large number of diverse variants of a parental sequence or sequences. In some preferred embodiments of the invention, the modification technique (e.g., some form of shuffling) is used to generate a library of variants that is then screened for a modified polynucleotide or pool of modified polynucleotides encoding some desired functional attribute, e.g., improved HAT activity. Exemplary enzymatic activities that can be screened for include, but are not limited to, catalytic rates (conventionally characterized in terms of kinetic constants such as k_cat and K_M), substrate specificity, and susceptibility to activation or inhibition by substrate, product or other molecules (e.g., inhibitors or activators) and the maximum velocity of an enzymatic reaction when the binding site is saturated with substrate (Vmax).

EXAMPLES

[0221] The present invention is further defined in the following Examples, in which all parts and percentages are by weight and degrees are Celsius, unless otherwise stated. It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions.

Example 1

Composition of cDNA Libraries; Isolation and Sequencing of cDNA Clones

[0222] cDNA libraries representing mRNAs from various corn tissues were prepared. The characteristics of the libraries are described in Table 1.

[0223] cDNA libraries may be prepared by any one of many available methods. For example, the cDNAs may be introduced into plasmid vectors by first preparing the cDNA libraries in Uni-ZAP® XR vectors according to the manufacturer's protocol (Stratagene Cloning Systems, La Jolla, Calif.). The Uni-ZAP® XR libraries are converted into plasmid libraries according to the protocol provided by Stratagene. Upon conversion, cDNA inserts will be contained in the plasmid vector pBluescript. In addition, the cDNAs may be introduced directly into precut Bluescript II SK(+) vectors (Stratagene) using T4 DNA ligase (New England Biolabs), followed by transfection into DH10B cells according to the manufacturer's protocol (GIBCO BRL Products). Once the cDNA inserts are in plasmid vectors, plasmid DNAs are prepared from randomly picked bacterial colonies containing recombinant pBluescript plasmids, or the insert cDNA sequences are amplified via polymerase chain reaction using primers specific for vector sequences flanking the inserted cDNA sequences. Amplified insert DNAs or plasmid DNAs are sequenced in dye-primer sequencing reactions to generate partial cDNA sequences (expressed sequence tags or "ESTs"; see Adams et al., (1991) Science 252:1651-1656). The resulting ESTs are analyzed using a Perkin Elmer Model 377 fluorescent sequencer.

TABLE-US-00002 TABLE 1 cDNA Libraries and clones containing NAR2-like sequences from Corn Library Tissue Clone Cnr1c Corn (Zea mays). Plants were Nitrogen cnr1c.pk003.m9.f:fis starved until all seed reserves were depleted of a Nitrogen source. Plants were induced with addition of Nitrogen, then samples were collected at 30 min-1 hr and 2 hr after Nitrogen. Cbn2 Corn (Zea mays L.) developing kernel cbn2.pk0042.g4:fis two days after pollination

Example 2

Identification of cDNA Clones

[0224] cDNA clones encoding components associated with nitrate transport were identified by conducting BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410;) and are shown in Table 1.

[0225] cDNA clones encoding transporters or components associated with nitrate transport can be identified by conducting BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410;) searches for similarity to sequences contained in the BLAST "nr" database (comprising all non-redundant GenBank CDS translations, sequences derived from the 3-dimensional structure Brookhaven Protein Data Bank, the last major release of the SWISS-PROT protein sequence database, EMBL, and DDBJ databases). The cDNA sequences obtained can be analyzed for similarity to all publicly available DNA sequences contained in the "nr" database using the BLASTN algorithm provided by the National Center for Biotechnology Information (NCBI). The DNA sequences can be translated in all reading frames and compared for similarity to all publicly available protein sequences contained in the "nr" database using the BLASTX algorithm (Gish and States (1993) Nature Genetics 3:266-272) provided by the NCBI. For convenience, the P-value (probability) of observing a match of a cDNA sequence to a sequence contained in the searched databases merely by chance as calculated by BLAST are reported herein as "pLog" values, which represent the negative of the logarithm of the reported P-value. Accordingly, the greater the pLog value, the greater the likelihood that the cDNA sequence and the BLAST "hit" represent homologous proteins.

Example 3

Identification and Sequencing of Corn High Affinity Nitrate Transporters (HAT4 and HAT5)

[0226] In order to identify homologs of HATs, a public HAT gene (Genbank accession number AY129953), was used to screen Iowa State University MAGI version 2.31 maize genome assembly. A partial clone, MAGI 17514 that showed 85% identity at the nucleotide level and appeared to be a previously unidentified HAT was identified using Blast in the ISU MAGI assembly. This sequence was used to screen the Genbank GSS dataset and some additional homologs of the MAGI sequence were identified; these added about 0.5 kb to the sequence. The GSS dataset consists of sequences set forth in general identification numbers: 33941728, 34245424, 32105143, 34245411, 34082540 and 33992813. The translation of the assembly covered about one half of the gene, at the 3' end. It completely lacked the 5' half of the gene.

[0227] In order to isolate the full length HAT4 sequence, BAC clones from two BAC libraries derived from the Maize B73 inbred line were screened using PCR. The libraries had previously been constructed by partial digestion of genomic DNA and inserted in the BamHI and EcoRI sites of the pCUGI (Tomkins, J. P., et al. 2002. Construction and characterization of a deep-coverage bacterial artificial chromosome library for maize. Crop Science 42:928-933) and pTARBAC (pTARBAC2.1 library, Osoegawa, K., et al, Construction Of New Maize, Bovine, Equine And Zebrafish Bac Libraries. Plant And Animal Genome Conference Proceedings. 2001). To facilitate a PCR-based screening, a set of 36 four-dimensional superpools was requested from Amplicon Express (Amplicon Express, 1610NE Eastgate Blvd Pullman, Wash. 99163). Each superpool was derived after the independent growth, isolation and pooling of 4608 clones, more than 165,000 arrayed BAC clones in total. Superpools were subject to PCR reactions, followed by fragment plus-minus determination in agarose gel electrophoresis. PCR primers were designed to amplify a 495-bp fragment located 289 bp downstream the stop codon of a HAT homolog located at the Tigr assembly ID AZM4_--32787, which is identical to the sequences assembled from the MAGI and GSS databases described above. PCR reactions were performed with 5 ng Template DNA in a 10-μL reaction that included 5 μL of Hotstar Taq Polymerase Mix (Qiagen) and 5 pmol of the forward and reverse primers (SEQ ID NO:1 and SEQ ID NO:2, respectively). Cycle conditions were an initial denaturation step at 95° C. for 15 minutes, followed by 35 cycles of 95° C. for 30 seconds, 60° C. for 30 seconds and 72° C. for 1 minute. A second round of PCR was performed in matrix plates consisting of lower-complexity combinatorial pools derived from clones represented in positive pools. This narrowed down the positives to particular clones. Two clones, bacc.pk139.d24 and bacc.pk142.b21, were identified and confirmed by PCR analysis. Clone bacc.pk139.d24 was used in subsequent work.

[0228] BAC DNA from clone bacc.pk139.d24 was isolated from overnight 250-ml 2xYT+cloramphenicol cultures using a modified alkaline lysis method. Cells were harvested by centrifugation and resuspended in 20 ml of 10-mM EDTA, then lysed by gently adding 40 ml of 0.2-N NaOH/1-% SDS and neutralized with 30 ml of cold 3-M potassium acetate (pH 4.8). Cell debris were removed by centrifugation at 4° C. 15 minutes at 15000×g, followed by filtration through Miracloth. DNA in supernatant was precipitated with 0.7 volumes of isopropanol and resuspended in 9 ml of 50-mM Tris/50-mM EDTA, mixed with 4.5 ml of 7.5-M potassium acetate, placed at -70° C., thawed and centrifuged for 20 minutes at 3500×g. The supernatant was decanted, precipitated with ethanol and resuspended in 0.7 ml of 50-mM Tris/50-mM EDTA. DNase-free RNase A was added to a final concentration of 150 μg/ml and incubated 1 hour at 37° C., followed by phenol:chloroform extraction and ethanol precipitation. Final DNA was resuspended in a total of 400 μl sterile nuclease-free water. DNA insert size, quantity and quality was assessed by Pulsed Field Gel Electrophoresis using a CHEF-Mapper III (Bio-Rad). For confirmatory BAC end sequencing, the T7 (SEQ ID NO:3) and SP6 (SEQ ID NO: 4) primers were used using sequencing conditions described below.

[0229] The general strategy to obtain double-strand, contiguous sequence information along the HAT4 gene was by walking from the known "start" sequence defined by the PCR identification primers, previously described. BAC bacc.pk139.d24 DNA was used as template. Sequencing was performed in a ABI3730 capillary sequencer according to manufacturer protocols. Sequencing reactions consisted of 2 μL of BigDye V3.1Terminator mix (Applied Biosystems), 2 μL of dilution buffer (600 mM Tris HCl pH 9.0, 15 mM MgCl2), 20 μmol of primer, and approximately 1 μg of template DNA in a final reaction volume of 20 μL. Cycle conditions were an initial denaturation at 95° C. for 5 minutes, followed by 99 cycles of 95° C. for 30 seconds, 58° C. for 30 seconds and 64° C. for 4 minutes. Some hard-to-read regions had to be re-sequenced using special cycle and reaction conditions. Excess dye terminator was removed by ethanol precipitation. Trace evaluation, base calling and assembly was based on Phred/Phrap software (Ewing et al. (1998) Genome Res. 8:186-194; Ewing et al. (1998) Genome Res. 8:175-185). Consed (Gordon et al. (1998) Genome Res. 8:195-202) was used for assembly analysis. After every sequence walking step, primers were designed at the ends, avoiding regions of high homology to other genes and to DNA repeats. Homology search was performed using the BLAST program (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410) against gss, TIGR 4.0, nonredundant, EST, and protein databases (Altschul et al. 1990). Vector NTI was used for primer design and primers were synthesized commercially by MWG Biotech. Primers (SEQ ID NO: 5 through SEQ ID NO: 33) were designed, tested and used to cover region including the HAT gene. SEQ ID NO: 34 describes the genomic sequence containing the HAT 4 gene. SEQ ID NOs: 35 and 36 describe the coding nucleotide and amino acid sequence of the corn HAT4, respectively.

[0230] SEQ ID NOs: 37 and 38 show the 2014 bp and 1014 bp putative promoter sequences of the HAT4 gene.

[0231] The HAT-5 family was identified via blast homology to the public HATs. One 3' clone cco1n.pk072.i13 had homology to MAGI_--56254, which appeared to represent the entire sequence. The TIGR assembly AZM4_--2103 corresponded well to the MAGI clone. Databases containing nitrogen induced libraries were re-blasted using this clone and clone cfp4n.pk008.p6 was identified. This clone was sequenced and contains the complete HAT5 gene sequence (SEQ ID NO:91 and 92).

Example 4

Identification and Sequencing of an Additional Corn High Affinity Nitrate Transporter (HAT 7)

[0232] A public HAT gene (HAT1, Genbank accession number AY129953) was used to search with Blast, Genbank maize genomic survey sequences (GSS) and maize genomic assemblies (Iowa State University MAGI and Tigr), to try to identify paralogs of AY129953. Along with the HAT4 gene (Example 3) there were other more distant homologs, including MAGI_--65216 which corresponded to AZM4_--79242, which contained slightly more sequence information than MAGI 65216). Neither of these two clones contained a start Methionine. AN additional hit to AZM4_--79246 exhibited similar percent identity when compared to AY129953. AZM4_--79246 encoded a start Methionine at nucleotide 2264-2266 and approximately 110 amino acids of coding sequence. Further examination showed that these two assemblies shared clone mates, OGUKX93 and OGUCS47 from the Tigr methylation filtrated library. Therefore it was assumed that AZM4_--79242 and AZM4_--79246 encode the same gene but have no sequence overlap.

[0233] In order to retrieve the full length sequence, PCR was performed using two different forward and two different reverse primers (SEQ ID NOs: 39, 40 and 41, 42, repectively) with T3 (SEQ ID NO: 43) and T7 extensions (SEQ ID NO: 44 at the 5' and 3' end, respectively. HotStart PCR, with an annealing temperature of 58° C. was performed using DNA from eight maize inbred lines (B73, Co159, GT119, Mo17, T218, Oh43 and W23) as templates. All 32 PCR reaction products were run on a agarose 1×TBE gel, excised and cleaned up and sequenced on a 3100 ABI Capillary Sequencer using methods known to those of ordinary skill in the art. The sequences were aligned and the missing sequence information was retrieved. The complete nucleotide sequence of the HAT7 gene is shown in SEQ ID NO: 45. SEQ ID NOs: 46 and 47 describe the 2263 bp and 1263 bp putative promoter sequences of the HAT7 gene and SEQ ID NOs: 48 and 49 describe the coding nucleotide and amino acid sequence of the corn HAT7, respectively.

Example 5

Characterization of Polypeptides Encoding High Affinity Nitrate Transporter

[0234] The data in Table 2 represent a calculation of the percent identity of the amino acid sequences set forth in SEQ ID NOs: 36 and 49 and the Oryza sativa sequences (NCBI General Identifier Nos. 34913806 and 50904699).

TABLE-US-00003 TABLE 2 Percent Identity of Amino Acid Sequences Deduced From the Nucleotide Sequences of cDNA Clones Encoding Polypeptides Homologous to High Affinity Nitrate Transporter (HAT) Percent Identity to SEQ ID NO. 34913806 50904699 36 38.0 75.3 49 78.2 39.4

[0235] Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. Sequence alignments and BLAST scores and probabilities indicate that the nucleic acid fragments comprising the instant cDNA clones encode corn high affinity nitrogen transporters.

Example 6

Identification and Sequencing of Corn Nitrogen Transport Related Genes, (NAR2-1 & NAR2-2)

[0236] Examination of blast hits from the maize root library cnr1c, described in Example 1 and Table 2, showed a number of Nitrogen transport related genes. Blast hits were searched with keywords such as nitrate, nitrogen, and transporter. A few of these were homologous to NCBI Accession number: CAC36942, a putative component of high affinity nitrate transporter (NAR2 gene). A TblastN search of maize ESTs, using the sequence of CAC36942 as a query, produced a number of significant hits from different maize libraries. The most 5' clone was identified by aligning the full-length query and the blast hits. A clone from the cnr1c library (cnr1c.pk003.m9.f) showed a methionine that was in the same region as the start methionine from CAC36942. This clone also showed an in frame stop codon upstream of the methionine. This clone was submitted for standard full insert sequencing (FIS) and contained the 971 bp of the NAR2.1, spanning nucleotides 591 through 1561 of SEQ ID NO: 53. SEQ ID NO: 53 shows the 1561 bp sequence of the NAR2.1 gene, which was assembled from the sequence information obtained from clone cnr1c.pk003.m9.f:fis and from Tigr sequence AZM4_--81138. SEQ ID NOs: 54 and 55 show the coding nucleotide and amino acid sequence of the NAR2.1 gene, respectively. SEQ ID NO: 56 shows 756 bp of the putative promoter of the NAR2.1. Using CAC36942 as a query also showed a different NAR2 homolog, cbn2.pk0042.g4. This clone also had a start Methionine, but because of the quality of the EST sequence the homology to CAC36942 was short. A complete version (Tigr clone AZM4_--1475) of this family member was identified by searching the Tigr maize genomic assembly using cbn2.pk0042.g4 as a query. SEQ ID NOs: 57 and 58 show the coding nucleotide and amino acid sequence of the NAR2.2 (Tigr clone AZM4_--1475), repectively.

[0237] NAR2.1 Promoter Isolation

[0238] The sequence information on the NAR2.1 promoter was extended further upstream by performing Genome Walker® DNA walking (BD BioSciences). This method employs PCR to facilitate the cloning of unknown genomic DNA sequences adjacent to a known sequence. First, pools of unknown genomic DNA were digested with different restriction enzymes that leave blunt ends. Each pool was ligated to adaptors to create Genome Walker" libraries. Eight different corn HG11 libraries were obtained. These libraries were digested with the following restriction enzymes: StuI, EcoRV, PmlI, PvulI, ScaI, DraI, SmaI, and PmeI.

[0239] Then two rounds of nested PCR amplification per library were performed. For the first round the outer adaptor primer (AP1, provided with kit) and the Nar2.1 specific outer primer (SEQ ID NO: 59) were used.

[0240] PCR was performed using the Advantage®-GC Genomic Polymerase Mix (BD Biosciences) in a 50 μL reaction containing 1 μL I library DNA, 0.5 μL each primer (10 μM), 4 μL dNTPs (2.5 mM), 2.2 μL Mg (OAc)₂, 10 μL I 15×GC Genomic PCR Reaction Buffer, 10 μL GC-Melt (5M), 20.8 μL ddH₂O, and 1 μL Advantage-GC Genomic Polymerase. The cycling conditions were as follows: 7 cycles of denaturation at 94° C. for 25 seconds and annealing/extension at 72° C. for 6 minutes followed by 32 cycles of denaturation at 94° C. for 25 seconds and annealing/extension at 67° C. for 6 minutes capped off by annealing/extension at 67° C. for 7 minutes.

[0241] The primary PCR product was then diluted 1:50 and 1 μL served as the template for the second round of PCR which used the same PCR set-up as the first round. The second round primers were the inner adaptor primer (AP2, provided with the kit) and the Nar2.1 specific inner primer (SEQ ID NO: 60). The cycling conditions for the second round were as follows: 5 cycles of denaturation at 94° C. for 25 seconds and annealing/extension at 72° C. for 6 minutes followed by 25 cycles of denaturation at 94 C for 25 seconds and annealing/extension at 67° C. for 6 minutes capped off by annealing/extension at 67° C. for 7 minutes.

[0242] A major PCR product (about 3 kb) was observed in the StuI library. This band was cut-out of the gel and purified using the Qiaquick Gel Extraction Kit (Qiagen) and ligated to a pGEM®-T Easy Vector (Promega). The 20 μL ligation reaction was as follows: 10 μL 2× Rapid Ligation Buffer, 1 μL pGEM®-T Easy Vector (50 ng), 1 μL T4 DNA Ligase (3 Weiss units/μL), and 8 μL insert DNA (13 ng/μL). The reaction was incubated at 4° C. overnight.

[0243] The ligation product was transformed into Max Efficiency DH10B (Invitrogen) competent cells. One μL of ligate was added to 20 μL of cells and put on ice for 30 minutes. The cells were heat shocked at 42° C. for 45 seconds and then placed again on ice for 2 minutes. The cells were added to 1 mL of SOC and placed on a shaker at 250 rpm for 1 hr at 37° C. Then, 100 μL of cells were plated onto LB media with Ampicillin, IPTG, and X-Gal to allow for blue/white selection. Only one white colony was obtained.

[0244] Plasmid DNA was purified using the Plasmid Mini Kit (Qiagen). The plasmid insert representing the NAR2 upstream promoter region was sequenced using standard primers (SP6 and T7) and custom primers (SEQ ID NOs: 61, 62, 63 and 64). SEQ ID NO: 65 shows the sequence of the additional 2917 bp putative NAR 2.1 promoter.

[0245] The sequence of the complete NAR2.1 gene is shown in SEQ ID NO: 66.

Example 7

Expression Pattern of Polypeptides of Instant Application

[0246] The expression pattern of high affinity nitrate transporters (HAT) and other polypeptides (NAR) required for high affinity nitrate transport was analyzed via Lynx MPSS Brenner et al (2000) Proc Natl Acad Sci USA 97:1665-70).

[0247] The expression patterns of NAR2.1 and HAT 1 genes are similar across more than 200 libraries as studied via Lynx MPSS (Brenner et al (2000) Proc Natl Acad Sci USA 97:1665-70). They are both expressed only in the cortical cylinder of the root tissue and are similarly induced by nitrate, indicating that the polypeptide products of these two genes form a functional complex for nitrate transport in maize roots.

[0248] Tissue-Specific Expression of NAR2.1 and HAT-1 in Maize:

[0249] Of the 210 libraries from different tissues encompassing the whole of maize plant, NAR2.1 and HAT-1 are expressed only in the root libraries. This indicates the root-specific function for each of these genes.

[0250] Expression analysis of NAR2.1 and HAT-1 in maize tissues. MPSS tag abundances were averaged over different tissue libraries. The number of libraries for each tissue was: anther, 3; ear, 15; kernel, 44; leaf, 39; pollen, 1; root, 36; silk, 9; stalk, 19; and tassel, 14.

[0251] Induction of Nitrate Uptake and Localization within Maize Roots:

[0252] Among the root libraries derived from an inbred line A63, the expression of both NAR2.1 and HAT-1 is similarly induced by nitrate.

[0253] Corn roots from etiolated seedlings obtained 7-days after growing in paper rolls in water, were harvested and subjected to different treatments in parallel. The freshly harvested roots were kept on ice as controls. The roots were incubated in an aerated solution containing different nutrients for different lengths of time and then either quickly frozen in liquid N and stored at -80° C. until used for expression analyses or saved between two layers of wet paper towels in ice for further manipulation. A batch of roots that had been treated for four hours in nitrate was manually dissected into cortical cylinder and stele.

[0254] Response of NAR2.1 and HAT 1 expression to different nutrient treatments. The roots were treated for either half hour or four hours in a medium containing either 1 mM nitrate (0.5 mM KNO₃ and 0.25 mM Ca(NO₃)₂) or 1 mM chloride (0.5 mM KCl and 0.25 mM CaCl₂). A batch of roots treated for 4 hours with nitrate was separated into cortical cylinder and stele and subjected to MPSS.

[0255] Both the NAR2.1 and HAT 1 genes from maize exhibit a similar response to nitrate (N) in the incubation medium which is incremental with time when compared to the parallel control roots incubated in a chloride solution. Also, both these genes are nearly exclusively located in the cortical sleeve and not in the stele. Their similar response to nitrate and their localization strongly indicate that the protein products of these genes make a functional nitrate transport complex in maize roots.

[0256] Opposite Regulation of Expression of NAR2.1 in Illinois High Protein (IHP) and Illinois Low Protein (ILP) Maize Lines:

[0257] IHP and ILP are two sets of lines that are derived from a maize population after ˜100 years of divergent selection for grain protein in the high and low grain protein directions, respectively (Uribelarrea et al., 2004). Whereas IHP grains contain >20% protein, those of ILP contain <5%. The roots of these two lines were subjected to Lynx MPSS after various treatments.

[0258] Roots were either kept in a nitrate solution all the time, starved for two hours for nitrate, or placed in nitrate solution after two hour starvation. Whereas NAR2.1 in IHP responded to nitrate treatment like A63, ILP exhibited an opposite response Given the level of expression of this gene in ILP in nitrate starved roots, which is similar to that of IHP roots kept in nitrate, these results suggest that mechanisms to respond to nitrate in both the directions do exist in maize. However, the mechanism for positive response appears to have been selected as indicated by similar response between IHP and A63, an inbred line with normal grain protein content of ˜10%.

[0259] Only IHP contained the tag for HAT 1 sequence and showed a similar pattern of expression as for NAR2.1, lending further support to the aforementioned suggestion that NAR2.1 and HAT 1 form a functional complex in maize roots.

[0260] Expression of Other HAT Genes in A63:

[0261] HAT 4G was expressed at >10 ppm only in four libraries, all derived from the root tissue. Thus, this gene appears to be root-specific. HAT 7 is expressed in chilled seedlings and three leaf libraries, suggesting that this gene may encode a protein for nitrate uptake from the xylem apoplast into the leaf cells. It is expected that the HAT sequences of the instant application form a functional nitrate transport complex with a NAR sequence.

Example 8

Confirmation of Function of the High Affinity Nitrate Transporters and Polypeptides Required for High Affinity Nitrate Transport Using the TUSC Mutant Population

[0262] The full genomic sequence for the high affinity nitrate transporter locus can be used to design primers to screen for Mu-insertion mutants in the TUSC population (U.S. Pat. No. 5,962,764, issued Oct. 5, 1999). The pooled TUSC population can be screened with gene specific primers. Alleles of the corn high affinity nitrate transporters and polypeptides required for high affinity nitrate transport can be recovered from this screen, and characterized. Furthermore, function of the sequences of the instant application can be confirmed by complementation studies.

Example 9

Expression of Recombinant DNA Constructs in Monocot Cells

[0263] A recombinant DNA construct comprising a cDNA encoding the instant polypeptides in sense orientation with respect to the maize 27 kD zein promoter that is located 5' to the cDNA fragment, and the 10 kD zein 3' end that is located 3' to the cDNA fragment, can be constructed. The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites (NcoI or SmaI) can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the digested vector pML103 as described below. Amplification is then performed in a standard PCR. The amplified DNA is then digested with restriction enzymes NcoI and SmaI and fractionated on an agarose gel. The appropriate band can be isolated from the gel and combined with a 4.9 kb NcoI-SmaI fragment of the plasmid pML103. Plasmid pML103 has been deposited under the terms of the Budapest Treaty at ATCC (American Type Culture Collection, 10801 University Blvd., Manassas, Va. 20110-2209), and bears accession number ATCC 97366. The DNA segment from pML103 contains a 1.05 kb SalI-NcoI promoter fragment of the maize 27 kD zein gene and a 0.96 kb SmaI-SalI fragment from the 3' end of the maize 10 kD zein gene in the vector pGem9Zf(+) (Promega). Vector and insert DNA can be ligated at 15° C. overnight, essentially as described in Maniatis. The ligated DNA may then be used to transform E. coli XL1-Blue (Epicurian Coli XL-1 Blue®; Stratagene). Bacterial transformants can be screened by restriction enzyme digestion of plasmid DNA and limited nucleotide sequence analysis using the dideoxy chain termination method (Sequenase® DNA Sequencing Kit; U.S. Biochemical). The resulting plasmid construct would comprise a recombinant DNA construct encoding, in the 5' to 3' direction, the maize 27 kD zein promoter, a cDNA fragment encoding the instant polypeptides, and the 10 kD zein 3' region.

[0264] The recombinant DNA construct described above can then be introduced into corn cells by the following procedure. Immature corn embryos can be dissected from developing caryopses derived from crosses of the inbred corn lines H99 and LH132. The embryos are isolated 10 to 11 days after pollination when they are 1.0 to 1.5 mm long. The embryos are then placed with the axis-side facing down and in contact with agarose-solidified N6 medium (Chu et al. (1975) Sci. Sin. Peking 18:659-668). The embryos are kept in the dark at 27° C. Friable embryogenic callus consisting of undifferentiated masses of cells with somatic proembryoids and embryoids borne on suspensor structures proliferates from the scutellum of these immature embryos. The embryogenic callus isolated from the primary explant can be cultured on N6 medium and sub-cultured on this medium every 2 to 3 weeks.

[0265] The plasmid, p35S/Ac (obtained from Dr. Peter Eckes, Hoechst Ag, Frankfurt, Germany) may be used in transformation experiments in order to provide for a selectable marker. This plasmid contains the Pat gene (see European Patent Publication 0242236) which encodes phosphinothricin acetyl transferase (PAT). The enzyme PAT confers resistance to herbicidal glutamine synthetase inhibitors such as phosphinothricin. The pat gene in p35S/Ac is under the control of the 35S promoter from Cauliflower Mosaic Virus (Odell et al. (1985) Nature 313:810-812) and the 3' region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens.

[0266] The particle bombardment method (Klein et al. (1987) Nature 327:70-73) may be used to transfer genes to the callus culture cells. According to this method, gold particles (1 μm in diameter) are coated with DNA using the following technique. Ten μg of plasmid DNAs are added to 50 μL of a suspension of gold particles (60 mg per mL). Calcium chloride (50 μL of a 2.5 M solution) and spermidine free base (20 μL of a 1.0 M solution) are added to the particles. The suspension is vortexed during the addition of these solutions. After 10 minutes, the tubes are briefly centrifuged (5 sec at 15,000 rpm) and the supernatant removed. The particles are resuspended in 200 μL of absolute ethanol, centrifuged again and the supernatant removed. The ethanol rinse is performed again and the particles resuspended in a final volume of 30 μL of ethanol. An aliquot (5 μL) of the DNA-coated gold particles can be placed in the center of a Kapton® flying disc (Bio-Rad Labs). The particles are then accelerated into the corn tissue with a Biolistic® PDS-1000/He (Bio-Rad Instruments, Hercules Calif.), using a helium pressure of 1000 psi, a gap distance of 0.5 cm and a flying distance of 1.0 cm.

[0267] For bombardment, the embryogenic tissue is placed on filter paper over agarose-solidified N6 medium. The tissue is arranged as a thin lawn and covered a circular area of about 5 cm in diameter. The petri dish containing the tissue can be placed in the chamber of the PDS-1000/He approximately 8 cm from the stopping screen. The air in the chamber is then evacuated to a vacuum of 28 inches of Hg. The macrocarrier is accelerated with a helium shock wave using a rupture membrane that bursts when the He pressure in the shock tube reaches 1000 psi.

[0268] Seven days after bombardment the tissue can be transferred to N6 medium that contains gluphosinate (2 mg per liter) and lacks casein or proline. The tissue continues to grow slowly on this medium. After an additional 2 weeks the tissue can be transferred to fresh N6 medium containing gluphosinate. After 6 weeks, areas of about 1 cm in diameter of actively growing callus can be identified on some of the plates containing the glufosinate-supplemented medium. These calli may continue to grow when sub-cultured on the selective medium.

Plants can be regenerated from the transgenic callus by first transferring clusters of tissue to N6 medium supplemented with 0.2 mg per liter of 2,4-D. After two weeks the tissue can be transferred to regeneration medium (Fromm et al. (1990) Bio/Technology 8:833-839).

Example 10

Expression of Recombinant DNA Constructs in Dicot Cells

[0269] A seed-specific expression cassette composed of the promoter and transcription terminator from the gene encoding the α-subunit of the seed storage protein phaseolin from the bean Phaseolus vulgaris (Doyle et al. (1986) J. Biol. Chem. 261:9228-9238) can be used for expression of the instant polypeptides in transformed soybean. The phaseolin cassette includes about 500 nucleotides upstream (5') from the translation initiation codon and about 1650 nucleotides downstream (3') from the translation stop codon of phaseolin. Between the 5' and 3' regions are the unique restriction endonuclease sites Nco I (which includes the ATG translation initiation codon), Sma I, Kpn I and Xba I. The entire cassette is flanked by Hind III sites.

[0270] The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the expression vector. Amplification is then performed as described above, and the isolated fragment is inserted into a pUC18 vector carrying the seed expression cassette.

[0271] Soybean embroys may then be transformed with the expression vector comprising sequences encoding the instant polypeptides. To induce somatic embryos, cotyledons, 3-5 mm in length dissected from surface sterilized, immature seeds of the soybean cultivar A2872, can be cultured in the light or dark at 26° C. on an appropriate agar medium for 6-10 weeks. Somatic embryos which produce secondary embryos are then excised and placed into a suitable liquid medium. After repeated selection for clusters of somatic embryos which multiplied as early, globular staged embryos, the suspensions are maintained as described below.

[0272] Soybean embryogenic suspension cultures can maintained in 35 mL liquid media on a rotary shaker, 150 rpm, at 26° C. with florescent lights on a 16:8 hour day/night schedule. Cultures are subcultured every two weeks by inoculating approximately 35 mg of tissue into 35 mL of liquid medium.

[0273] Soybean embryogenic suspension cultures may then be transformed by the method of particle gun bombardment (Klein et al. (1987) Nature (London) 327:70-73, U.S. Pat. No. 4,945,050). A DuPont Biolistic® PDS1000/HE instrument (helium retrofit) can be used for these transformations.

[0274] A selectable marker gene which can be used to facilitate soybean transformation is a recombinant DNA construct composed of the 35S promoter from Cauliflower Mosaic Virus (Odell et al. (1985) Nature 313:810-812), the hygromycin phosphotransferase gene from plasmid pJR225 (from E. coli; Gritz et al. (1983) Gene 25:179-188) and the 3' region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens. The seed expression cassette comprising the phaseolin 5' region, the fragment encoding the instant polypeptides and the phaseolin 3' region can be isolated as a restriction fragment. This fragment can then be inserted into a unique restriction site of the vector carrying the marker gene.

[0275] To 50 μL of a 60 mg/mL 1 μm gold particle suspension is added (in order): 5 μL DNA (1 μg/μL), 20 pLspermidine (0.1 M), and 50 μL CaCl₂ (2.5 M). The particle preparation is then agitated for three minutes, spun in a microfuge for 10 seconds and the supernatant removed. The DNA-coated particles are then washed once in 400 μL 70% ethanol and resuspended in 40 μL of anhydrous ethanol. The DNA/particle suspension can be sonicated three times for one second each. Five μL of the DNA-coated gold particles are then loaded on each macro carrier disk.

[0276] Approximately 300-400 mg of a two-week-old suspension culture is placed in an empty 60×15 mm petri dish and the residual liquid removed from the tissue with a pipette. For each transformation experiment, approximately 5-10 plates of tissue are normally bombarded. Membrane rupture pressure is set at 1100 psi and the chamber is evacuated to a vacuum of 28 inches mercury. The tissue is placed approximately 3.5 inches away from the retaining screen and bombarded three times. Following bombardment, the tissue can be divided in half and placed back into liquid and cultured as described above.

[0277] Five to seven days post bombardment, the liquid media may be exchanged with fresh media, and eleven to twelve days post bombardment with fresh media containing 50 mg/mL hygromycin. This selective media can be refreshed weekly. Seven to eight weeks post bombardment, green, transformed tissue may be observed growing from untransformed, necrotic embryogenic clusters. Isolated green tissue is removed and inoculated into individual flasks to generate new, clonally propagated, transformed embryogenic suspension cultures. Each new line may be treated as an independent transformation event. These suspensions can then be subcultured and maintained as clusters of immature embryos or regenerated into whole plants by maturation and germination of individual somatic embryos.

Example 11

Expression of Recombinant DNA Construct in Microbial Cells

[0278] The cDNAs encoding the instant polypeptides can be inserted into the T7 E. coli expression vector pBT430. This vector is a derivative of pET-3a (Rosenberg et al. (1987) Gene 56:125-135) which employs the bacteriophage T7 RNA polymerase/T7 promoter system. Plasmid pBT430 was constructed by first destroying the EcoR I and Hind III sites in pET-3a at their original positions. An oligonucleotide adaptor containing EcoR I and Hind III sites was inserted at the BamH I site of pET-3a. This created pET-3aM with additional unique cloning sites for insertion of genes into the expression vector. Then, the Nde I site at the position of translation initiation was converted to an Nco I site using oligonucleotide-directed mutagenesis. The DNA sequence of pET-3aM in this region, 5'-CATATGG, was converted to 5'-CCCATGG in pBT430.

[0279] Plasmid DNA containing a cDNA may be appropriately digested to release a nucleic acid fragment encoding the protein. This fragment may then be purified on a 1% NuSieve GTG® low melting agarose gel (FMC). Buffer and agarose contain 10 μg/ml ethidium bromide for visualization of the DNA fragment. The fragment can then be purified from the agarose gel by digestion with GELase® (Epicentre Technologies) according to the manufacturer's instructions, ethanol precipitated, dried and resuspended in 20 μL of water. Appropriate oligonucleotide adapters may be ligated to the fragment using T4 DNA ligase (New England Biolabs, Beverly, Mass.). The fragment containing the ligated adapters can be purified from the excess adapters using low melting agarose as described above. The vector pBT430 is digested, dephosphorylated with alkaline phosphatase (NEB) and deproteinized with phenol/chloroform as described above. The prepared vector pBT430 and fragment can then be ligated at 16° C. for 15 hours followed by transformation into DH5 electrocompetent cells (GIBCO BRL). Transformants can be selected on agar plates containing LB media and 100 μg/mL ampicillin. Transformants containing the gene encoding the instant polypeptides are then screened for the correct orientation with respect to the T7 promoter by restriction enzyme analysis.

[0280] For high level expression, a plasmid clone with the cDNA insert in the correct orientation relative to the T7 promoter can be transformed into E. coli strain BL21(DE3) (Studier et al. (1986) J. Mol. Biol. 189:113-130). Cultures are grown in LB medium containing ampicillin (100 mg/L) at 25° C. At an optical density at 600 nm of approximately 1, IPTG (isopropylthio-beta-galactoside, the inducer) can be added to a final concentration of 0.4 mM and incubation can be continued for 3 h at 25° C. Cells are then harvested by centrifugation and re-suspended in 50 μL of 50 mM Tris-HCl at pH 8.0 containing 0.1 mM DTT and 0.2 mM phenyl methylsulfonyl fluoride. A small amount of 1 mm glass beads can be added and the mixture sonicated 3 times for about 5 seconds each time with a microprobe sonicator. The mixture is centrifuged and the protein concentration of the supernatant determined. One μg of protein from the soluble fraction of the culture can be separated by SDS-polyacrylamide gel electrophoresis. Gels can be observed for protein bands migrating at the expected molecular weight.

Example 12

Electroporation of Aqrobacterium tumefaciens LBA4404

[0281] Electroporation competent cells (40 μL), such as Agrobacterium tumefaciens LBA4404 (containing PHP10523), are thawed on ice (20-30 min). PHP10523 contains VIR genes for T-DNA transfer, an Agrobacterium low copy number plasmid origin of replication, a tetracycline resistance gene, and a Cos site for in vivo DNA bimolecular recombination. PHP10523 is further described in Example 17. Meanwhile the electroporation cuvette is chilled on ice. The electroporator settings are adjusted to 2.1 kV. A DNA aliquot (0.5 μL parental DNA at a concentration of 0.2 μg-1.0 μg in low salt buffer or twice distilled H₂O) is mixed with the thawed Agrobacterium tumefaciens LBA4404 cells while still on ice. The mixture is transferred to the bottom of electroporation cuvette and kept at rest on ice for 1-2 min. The cells are electroporated (Eppendorf electroporator 2510) by pushing the "pulse" button twice (ideally achieving a 4.0 millisecond pulse). Subsequently, 0.5 mL of room temperature 2xYT medium (or SOC medium) are added to the cuvette and transferred to a 15 mL snap-cap tube (e.g., Falcon® tube). The cells are incubated at 28-30° C., 200-250 rpm for 3 h.

[0282] Aliquots of 250 μL are spread onto plates containing YM medium and 50 μg/mL spectinomycin and incubated three days at 28-30° C. To increase the number of transformants one of two optional steps can be performed:

[0283] Option 1: Overlay plates with 30 μL of 15 mg/mL rifampicin. LBA4404 has a chromosomal resistance gene for rifampicin. This additional selection eliminates some contaminating colonies observed when using poorer preparations of LBA4404 competent cells.

[0284] Option 2: Perform two replicates of the electroporation to compensate for poorer electrocompetent cells.

Identification of Transformants:

[0285] Four independent colonies are picked and streaked on plates containing AB minimal medium and 50 μg/mL spectinomycin for isolation of single colonies. The plates are incubated at 28° C. for two to three days. A single colony for each putative co-integrate is picked and inoculated with 4 mL of 10 g/L bactopeptone, 10 g/L yeast extract, 5 g/L sodium chloride and 50 mg/L spectinomycin. The mixture is incubate for 24 h at 28° C. with shaking. Plasmid DNA from 4 mL of culture is isolated using Qiagen Miniprep and an optional Buffer PB wash. The DNA is eluted in 30 μL. Aliquots of 2 μL are used to electroporate 20 μL of DH10b+20 μL of twice distilled H₂O as per above. Optionally a 15 μL aliquot can be used to transform 75-100 μL of Invitrogen Library Efficiency DH5a. The cells are spread on plates containing LB medium and 50 μg/mL spectinomycin and incubated at 37° C. overnight.

[0286] Three to four independent colonies are picked for each putative co-integrate and inoculated 4 mL of 2xYT medium (10 g/L bactopeptone, 10 g/L yeast extract, 5 g/L sodium chloride) with 50 μg/mL spectinomycin. The cells are incubated at 37° C. overnight with shaking. Next, isolate the plasmid DNA from 4 mL of culture using QIAprep® Miniprep with optional Buffer PB wash (elute in 50 μL). Use 8 μL for digestion with SalI (using parental DNA and PHP10523 as controls). Three more digestions using restriction enzymes BamHI, EcoRI, and HindIII are performed for 4 plasmids that represent 2 putative co-integrates with correct SalI digestion pattern (using parental DNA and PHP10523 as controls). Electronic gels are recommended for comparison.

Example 13

Transformation of Maize Using Agrobacterium

[0287] Agrobacterium-mediated transformation of maize is performed essentially as described by Zhao et al. in Meth. Mol. Biol. 318:315-323 (2006) (see also Zhao et al., Mol. Breed. 8:323-333 (2001) and U.S. Pat. No. 5,981,840 issued Nov. 9, 1999, incorporated herein by reference). The transformation process involves bacterium innoculation, co-cultivation, resting, selection and plant regeneration.

1. Immature Embryo Preparation:

[0288] Immature maize embryos are dissected from caryopses and placed in a 2 mL microtube containing 2 mL PHI-A medium.

2. Agrobacterium Infection and Co-Cultivation of Immature Embryos:

2.1 Infection Step

[0289] PHI-A medium of (1) is removed with 1 mL micropipettor, and 1 mL Agrobacterium suspension (including, but not limited to, the Agrobacterium described in Example 7) is added. The tube is gently inverted to mix. The mixture is incubated for 5 min at room temperature.

2.2 Co-Culture Step

[0290] The Agrobacterium suspension is removed from the infection step with a 1 mL micropipettor. Using a sterile spatula the embryos are scraped from the tube and transferred to a plate of PHI-B medium in a 100×15 mm Petri dish. The embryos are oriented with the embryonic axis down on the surface of the medium. Plates with the embryos are cultured at 20° C., in darkness, for three days. L-Cysteine can be used in the co-cultivation phase. With the standard binary vector, the co-cultivation medium supplied with 100-400 mg/L L-cysteine is critical for recovering stable transgenic events.

3. Selection of Putative Transgenic Events:

[0291] To each plate of PHI-D medium in a 100×15 mm Petri dish, 10 embryos are transferred, maintaining orientation and the dishes are sealed with parafilm. The plates are incubated in darkness at 28° C. Actively growing putative events, as pale yellow embryonic tissue, are expected to be visible in six to to eight weeks. Embryos that produce no events may be brown and necrotic, and little friable tissue growth is evident. Putative transgenic embryonic tissue is subcultured to fresh PHI-D plates at two-three week intervals, depending on growth rate. The events are recorded.

4. Regeneration of T0 Plants:

[0292] Embryonic tissue propagated on PHI-D medium is subcultured to PHI-E medium (somatic embryo maturation medium), in 100×25 mm Petri dishes and incubated at 28° C., in darkness, until somatic embryos mature, for about ten to eighteen days. Individual, matured somatic embryos with well-defined scutellum and coleoptile are transferred to PHI-F embryo germination medium and incubated at 28° C. in the light (about 80 pE from cool white or equivalent fluorescent lamps). In seven to ten days, regenerated plants, about 10 cm tall, are potted in horticultural mix and hardened-off using standard horticultural methods.

[0293] Media for Plant Transformation:

[0294] 1. PHI-A: 4 g/L CHU basal salts, 1.0 mL/L 1000× Eriksson's vitamin mix, 0.5 mg/L thiamin HCl, 1.5 mg/L 2,4-D, 0.69 g/L L-proline, 68.5 g/L sucrose, 36 g/L glucose, pH 5.2. Add 100 μM acetosyringone (filter-sterilized).

[0295] 2. PHI-B: PHI-A without glucose, increase 2,4-D to 2 mg/L, reduce sucrose to 30 g/L and supplemente with 0.85 mg/L silver nitrate (filter-sterilized), 3.0 g/L Gelrite®, 100 μM acetosyringone (filter-sterilized), pH 5.8.

[0296] 3. PHI--C: PHI-B without Gelrite® and acetosyringonee, reduce 2,4-D to 1.5 mg/L and supplemente with 8.0 g/L agar, 0.5 g/L 2-[N-morpholino]ethane-sulfonic acid (MES) buffer, 100 mg/L carbenicillin (filter-sterilized).

[0297] 4. PHI-D: PHI--C supplemented with 3 mg/L bialaphos (filter-sterilized).

[0298] 5. PHI-E: 4.3 g/L of Murashige and Skoog (MS) salts, (Gibco, BRL 11117-074), 0.5 mg/L nicotinic acid, 0.1 mg/L thiamine HCl, 0.5 mg/L pyridoxine HCl, 2.0 mg/L glycine, 0.1 g/L myo-inositol, 0.5 mg/L zeatin (Sigma, Cat. No. Z-0164), 1 mg/L indole acetic acid (IAA), 26.4 μg/L abscisic acid (ABA), 60 g/L sucrose, 3 mg/L bialaphos (filter-sterilized), 100 mg/L carbenicillin (filter-sterilized), 8 g/L agar, pH 5.6.

[0299] 6. PHI-F: PHI-E without zeatin, IAA, ABA; reduce sucrose to 40 g/L; replacing agar with 1.5 g/L Gelrite®; pH 5.6.

[0300] Plants can be regenerated from the transgenic callus by first transferring clusters of tissue to N6 medium supplemented with 0.2 mg per liter of 2,4-D. After two weeks the tissue can be transferred to regeneration medium (Fromm et al., Bio/Technology 8:833-839 (1990)). Transgenic T0 plants can be regenerated and their phenotype determined. T1 seed can be collected.

[0301] Furthermore, a recombinant DNA construct containing a validated Arabidopsis gene can be introduced into an elite maize inbred line either by direct transformation or introgression from a separately transformed line.

[0302] Transgenic plants, either inbred or hybrid, can undergo more vigorous field-based experiments to study yield enhancement and/or stability under nitrogen limiting and nitrogen non-limiting conditions.

[0303] Subsequent yield analysis can be done to determine whether plants that contain the validated Arabidopsis lead gene have an improvement in yield performance (under nitrogen limiting or non-limiting conditions), when compared to the control (or reference) plants that do not contain the validated Arabidopsis lead gene. Plants containing the validated Arabidopsis lead gene would have less yield loss relative to the control plants, preferably 50% less yield loss, under nitrogen limiting conditions, or would have increased yield relative to the control plants under nitrogen non-limiting conditions.

Example 14

Evaluating Compounds for their Ability to Inhibit the Activity of NitrateTransporters

[0304] The polypeptides described herein may be produced using any number of methods known to those skilled in the art. Such methods include, but are not limited to, expression in bacteria as described in Example 11, or expression in eukaryotic cell culture, in planta, and using viral expression systems in suitably infected organisms or cell lines. The instant polypeptides may be expressed either as mature forms of the proteins as observed in vivo or as fusion proteins by covalent attachment to a variety of enzymes, proteins or affinity tags. Common fusion protein partners include glutathione S-transferase ("GST"), thioredoxin ("Trx"), maltose binding protein, and C- and/or N-terminal hexahistidine polypeptide ("(His)₆"). The fusion proteins may be engineered with a protease recognition site at the fusion point so that fusion partners can be separated by protease digestion to yield intact mature enzyme. Examples of such proteases include thrombin, enterokinase and factor Xa. However, any protease can be used which specifically cleaves the peptide connecting the fusion protein and the enzyme.

[0305] Purification of the instant polypeptides, if desired, may utilize any number of separation technologies familiar to those skilled in the art of protein purification. Examples of such methods include, but are not limited to, homogenization, filtration, centrifugation, heat denaturation, ammonium sulfate precipitation, desalting, pH precipitation, ion exchange chromatography, hydrophobic interaction chromatography and affinity chromatography, wherein the affinity ligand represents a substrate, substrate analog or inhibitor. When the instant polypeptides are expressed as fusion proteins, the purification protocol may include the use of an affinity resin, which is specific for the fusion protein tag attached to the expressed enzyme or an affinity resin containing ligands, which are specific for the enzyme. For example, the instant polypeptides may be expressed as a fusion protein coupled to the C-terminus of thioredoxin. In addition, a (His)₆ peptide may be engineered into the N-terminus of the fused thioredoxin moiety to afford additional opportunities for affinity purification. Other suitable affinity resins could be synthesized by linking the appropriate ligands to any suitable resin such as Sepharose-4B. In an alternate embodiment, a thioredoxin fusion protein may be eluted using dithiothreitol; however, elution may be accomplished using other reagents which interact to displace the thioredoxin from the resin. These reagents include β-mercaptoethanol or other reduced thiol. The eluted fusion protein may be subjected to further purification by traditional means as stated above, if desired. Proteolytic cleavage of the thioredoxin fusion protein and the enzyme may be accomplished after the fusion protein is purified or while the protein is still bound to the ThioBond® affinity resin or other resin.

[0306] Crude, partially purified or purified enzyme, either alone or as a fusion protein, may be utilized in assays for the evaluation of compounds for their ability to inhibit enzymatic activation of the instant polypeptides disclosed herein. Assays may be conducted under well known experimental conditions that permit optimal enzymatic activity.

[0307] Assays that enable rapid screening for nitrate transport activity have been described in the literature, including, but not limited to an assay that measures ¹⁵N-enriched nitrate uptake into Xenopus oocytes expressing the proteins (Tong et al., The Plant J. (2005) 41:442-450).

Example 15

Expansion of the Linear Nitrate Uptake Range of Higher Plant HATS by Gene Shuffling

[0308] HATs are known to possess a low Km (in 10 to 100 μM range) and low Vmax (Doddema et al., Kinetics. Physiol. Plant. (1979) 45:332-338, Meharg et al., (1995) J. Membr. Biol. 145:49-66, Touraine et al., Plant Physiol. (1997) 114:137-144, Liu et al., Plant Cell. (1999) 11(5):865-874). Therefore, the uptake rate of HATs remains constant once the nitrate concentration reaches a level of about 2 to 3 fold higher than their Km.

[0309] The most relevant field nitrate concentration is around 2 to 5 mM on a typical modern corn farmland. Within this concentration range, the uptake rate of HATs is well saturated. Extending the linear nitrate uptake of HATs from very low to relevant field concentration would allow maize crop to fully utilize available nitrate for better growth and productivity. Such a transporter would also allow the crop plant to maintain the normal uptake efficiency at lower nitrate input by its enhanced ability to uptake fast at relatively lower nitrate concentration.

[0310] Various gene-shuffling methods (Stemmet WP, PNAS (1994) 91: 10747-10751, Crameri et al., Nature (1998) 391: 288-291, Ness et al., Nature Biotech. (1999) 17:893-896) can be used to generate different types of shuffled HATs libraries. For example, libraries can be generated by single gene and family gene shuffling. Additional diversities can be introduced by spiked oligos carrying amino acid mutations.

[0311] The shuffled HAT libraries can be functionally expressed in one of the heterologous hosts such as yeast, E. coli, and green algae. Preferably, the host lacks the nitrate assimilation pathway except for an endogenous or introduced nitrate reductase. Nitrate uptake rate by functionally expressed shufflants can be assayed by either direct measurement of depletion of nitrate in the assay medium via HPLC or other analytical means or by measurement of nitrite generated by nitrate reductase within the same cell. Nitrite concentration can be easily determined by colorimetrical assay (such as use of Greiss Reagent) or other analytical means (HPLC). Further characterization of the putative hits from screening various shuffled libraries can be achieved by measuring the uptake rates against different concentrations of nitrate. Such assay will provide uptake kinetic parameters of Km and Vmax.

[0312] Hits confirmed with improved properties can then be reshuffled to generate a second round of shuffled libraries and the aforementioned screening scheme can be used for identifying second round hits. This process can be repeated until several shuffled variants are identified that meet the desired kinetic properties.

Example 16

Isolation, Cloning and Sequencing of the Nar Promoter from the Maize B73 Inbred Line

Identification of a BAC Clone Carrying the Nar Gene

[0313] A BAC library derived from maize B71 inbred line was screened by PCR using the forward and reverse primers depicted in SEQ ID NOs: 75 and 76, respectively. Cycle conditions were an initial activation step at 95° C. for 15 minutes, followed by 35 cycles at 94° C. for 1 minute, 60° C. for 1 minute and 72° C. for 1 minute. Final extension was at 72° C. for 10 minutes. A 377 bp product was obtained. BAC clone ZMMBBb0521a1 was identified as carrying the Nar gene. Cloning of the Nar Promoter from Maize B73 Inbred Line The Nar promoter was cloned by PCR using the forward and reverse primer with restriction enzyme sites for BamHI and HindIII depicted in SEQ ID NOs: 77 and 78, repectively. To 1 μl diluted (1:100) BAC DNA from BAC clone ZMMBBb0521a1, 1 μl primer mix at a concentration of 10 μM each, 4 μl DNTPs at a concentration of 2.5 mM, 10 μl 5×HF buffer and 33.5 μl H₂O and 0.5 μl Phusion High Fidelity DNA Polymerase (Finnzymes) were added. Cycle conditions were an initial activation step at 98° C. for 30 seconds, followed by 35 cycles of 98° C. for 10 seconds, 63° C. for 30 seconds and 72° C. for 1 minute. Final Extension was at 72° C. for 10 minutes. A product of 3621 bp was obtained. The 3621 bp product was gel purified using the Qiaquick® Gel Extraction Kit (Qiagen) and eluted with 88 μl Elution Buffer. To the purified band 10 μl of buffer E (Promega) and 1 μl of each of the restriction enzyme, BamHI and Hind III (each at 10 U/μl) were added. The assay mixture was incubated at 37° C. for 3 hrs and cleaned up with Qiaquick® PCR Purification Kit (Qiagen). The pENTR-5' vector (SEQ ID NO: 85) was digested with BamHI and HindIII and dephosphorylated. The purified PCR band was inserted into the prepared pENTR-5' vector using the Epicentre Fast Link Kit. The ligation reaction mixture contained 1.5 μL buffer (10×), 1.5 μL ATP (10×), 1 μL ligase, 1 μL pENTR-5' vector (-10 ng/μL BamHI/HindllUdephosphorylated vector), 1 μL promoter insert (-30 ng) and 9 μL H20. The ligaton reaction was allowed to proceed for 15 minutes at room temperature and was stopped by incubating the mixture at 70° C. for 15 minutes. Transformation into Bacteria and PCR Screen for Insert 1 μL of the ligation mix was added to 20 μL of electro-competent cells (DH10B ElectroMax-Invitrogen) and the mixture was electroporated with a Gibco BRL Cell Porator, then 1 mL SOC media were added and the mixture was incubated in a shaker at 37° C. for 1 hr. 150 μL of cells were plated on LB plates with Kanamycin selection and grown overnight at 37° C. 12 colonies were picked and 30 μL LB media was added. The colonies were screened using PCR. To 1 μL colony DNA (colony/30 μL LB), 5 μL HotTaq 2× master mix (Qiagen), 1 μL (10 mM primer mix, SEQ ID NO: 77 and 78) and 3 μL dH₂O were added. Cycle conditions were an initial activation at 95° C. for 15 minutes, followed by 35 cycles of 95° C. for 50 seconds, 55° C. for 50 seconds and of 72° C. for 4 minutes. Final Extension was at 72° C. for 10 minutes.

Insert Sequencing

[0314] DNA carrying the insert was sequenced using the sequence primers depicted in SEQ ID NOs: 79-84. The sequence of the insert is shown in SEQ ID NO: 70. The vector construct carrying the 3621 bp insert was named PHP27621 and is shown in SEQ ID NO: 86 and FIG. 1.

Example 17

Testing the NAR Promoter in Transqenic Maize and Arabidopsis

[0315] Using Invitrogen's® gateway LR Clonase technology a MultiSite Gateway® LR Recombination Reaction was performed to create the corn NAR promoter::GUS::PINII, UBI::MO-PAT::P1NII and LTP2::DS-RED PINII JT binary vector (PHP27660, SEQ ID NO: 87 and FIG. 2). The vector PHP27660 contains the following expression cassettes:

[0316] 1. Ubiquitin promoter::MO-PAT::PINII terminator cassette expressing the PAT herbicide resistance gene used for selection during the transformation process.

[0317] 2. LTP2 promoter::DS-RED2::PinII terminator cassette expressing the DS-RED color marker gene used for seed sorting.

[0318] 3. NAR promoter::GUS::PINII terminator cassette expressing the GUS gene under control of the corn NAR promoter. Vector PHP27660 was electroporated using the protocol outlined in Example 16 into LBA4404 Agrobacterium cells containing PHP10523 by electroporation creating the final co-integrate vector PHP27860 (SEQ ID NO: 88 and FIG. 3) was then used for Agrobacterium-based maize transformation as described in Example 17. TO transgenic plants were sampled for GUS expression. Separately, the same vector (PHP27860) was also used for Arabidopsis transformation, following the standard inflorescence-dipping procedures. Transgenic events were selected by herbicide glufosinate spraying on the T1 seedlings. The herbicide-resistant T1 plants were sampled for GUS expression.

[0319] Leaf and root tissue samples were collected from transgenic plants at different time points, including seedling stage and at maturity. Freshly collected tissue samples were dissected into small pieces to facilitate penetration of the GUS staining solution. GUS histochemical staining was done following the standard protocol (Jefferson R A, Kavanagh T A, Bevan M W. 1987 GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6(13):3901-3907) incubating at 37° C. overnight.

No significant promoter activity was observed in transgenic maize and Arabidopsis tissues.

Example 18

Testing the Effects of Extraneous Junction Sequences on the NAR Promoter in Transqenic Maize and Arabidopsis

[0320] The Gateway cloning system leaves a short fragment of "foot-print" sequences between components, particularly a 21-bp ATT-B1 fragment between the NAR promoter and the GUS coding region. This has been shown to weaken or even abolish promoter activity in certain cases. This likely is related to the physical distance between basal promoter elements and the start codon. To determine if introducing the ATT-B1 site is negatively affecting the NAR promoter, a construct containing the corn NARpromoter::GUS::PINII cassette is built with a conventional cloning method, i.e., without the use of the Gateway system. Transgenic maize plants are produced via Agrobacterium-based transformation, and various tissue samples are collected for GUS expression study as described in Example 17.

Example 19

Testing the Maize NAR Promoter in a Deletion Series

[0321] The NAR gene has a nitrate-inducible and root-specific expression pattern.

[0322] To determine the fragments that determine NAR promoter activity and specificity, a series of constructs containing truncated NAR promoter fragments linked to the sequences for GUS and the PINII end are constructed and tested as described for the full length promoter in Examples 17 and 18.

[0323] Using BLASTN (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410;), sequences within the NAR promoter can be identified that might be important for enhancing or suppressing promoter activity. The sequence around 1.5 to 1.9 kb of the NAR promoter shows homology to another gene and a transposon element. Deletion of this fragment as shown in SEQ ID NO: 89 is therefore expected to add information on NAR promoter activity.

[0324] In addition truncation that reduce the length of the promoter as shown in SEQ ID NOs: 71, 72, 73, 74 and 90 can also be tested in the same way as described for the full length promoter in Examples 17 and 18. Additional promoter subfragments can be prepared by using primers derived from the 3.6 Kb NAR promoter sequence in PCR.

Example 20

Evaluation of Nitrate Uptake in Maize Using HAT and NAR Sequences and Combinations Thereof

[0325] The following maize expression constructs were prepared for evaluation of nitrate uptake in maize: PHP27280 (SEQ ID NO: 93 and FIG. 4), PHP27281 (SEQ ID NO:94 and FIG. 5), PHP27282 (SEQ ID NO: 95 and FIG. 6) and PHP27283 (SEQ ID NO:96 and FIG. 7).

Additional constructs comprising HAT sequences and combinations of HAT and Nar sequences will be prepared and tested for their ability to alter Nitrate transport. T0, T1 and subsequenct generations will be evaualted for alterd biomass and total ear weight under 1 mM nitrate conditions.

Sequence CWU 1

1

96125DNAArtificialPrimer 1ccaactggag tccaacaccc acaaa 25221DNAArtificialPrimer 2catgctgctc gtccactgcg g 21320DNAArtificialPrimer 3taatacgact cactataggg 20419DNAArtificialPrimer 4tatttaggtg acactatag 19520DNAArtificialPrimer 5atgttgttgg tggtgagctg 20618DNAArtificialPrimer 6acacgaggtt ggccatgc 18725DNAArtificialPrimer 7gtttgacacc ccttttctag caagg 25825DNAArtificialPrimer 8ccttgctaga aaaggggtgt caaac 25927DNAArtificialPrimer 9ggtcccgttt ggttagagag actaatc 271022DNAArtificialPrimer 10gcgcaacgaa atgcattggt ca 221125DNAArtificialPrimer 11aggggagaga agagaaaaag cgggt 251228DNAArtificialPrimer 12gctgcatgtt tacgactaca atctttgg 281325DNAArtificialPrimer 13tttgtgggtg ttggactcca gttgg 251423DNAArtificialPrimer 14tttgtgggtg ttggactcca gtt 231520DNAArtificialPrimer 15tttgtgggtg ttggactcca 201617DNAArtificialPrimer 16gggatgacgc cgaaggt 171717DNAArtificialPrimer 17cttcggcgtc atcccct 171817DNAArtificialPrimer 18aaggggatga cgccgaa 171917DNAArtificialPrimer 19ttcggcgtca tcccctt 172021DNAArtificialPrimer 20cacatcgccg tgggcatcct t 212118DNAArtificialPrimer 21aggatgccca cggcgatg 182221DNAArtificialPrimer 22cacatcgccg tgggcatcct t 212321DNAArtificialPrimer 23aaggatgccc acggcgatgt g 212418DNAArtificialPrimer 24tgccccgcgg ttagcaca 182518DNAArtificialPrimer 25tgtgctaacc gcggggca 182618DNAArtificialPrimer 26gcggttagca caaggatg 182718DNAArtificialPrimer 27catccttgtg ctaaccgc 182825DNAArtificialPrimer 28ggtagttggc gacggcgtgc cagag 252922DNAArtificialPrimer 29gcgacggcgt gccagagcac cc 223025DNAArtificialPrimer 30caggttctcc cggatgatgg ggatc 253126DNAArtificialPrimer 31gatccccatc atccgggaga acctgg 263225DNAArtificialPrimer 32gatccccatc atccgggaga acctg 253326DNAArtificialPrimer 33ccaggttctc ccggatgatg gggatc 26343924DNAZea mays 34ttcgagggca atgggttcca aagaatgtca tttgaattag acacttagtt atttatgaaa 60aggttttttc tccccgagtt aatttgcttc caaactataa ttaaccctaa gcaaggtgtt 120agttatttgt tttgacggtt tatatatccg tgttagcttg gtggctagct tgtatccatt 180tgacttgacg gcacatgcat gcatgcgtgg agtgcaccgt gcggcggttt gtgacgcggt 240gccaaacgtg caattgactc attgagtagt catcagcagg cttgcgatca ttagacacta 300acaagcatta atatttgctg catatatata tatacacaca catgcttcac tgacgacgct 360tgcaacttga tcttgttaat tattatatat cctaagcaca acgaacaaac cttagatatg 420cgaccatgcc ttgagtagag cgtgaaaaat agggggtgaa aaaaagggac gagtaattat 480agatgacact atttgatatt gtttaaagat gagataggga atgtgctgaa tagatcaatt 540tttaatcagg gatggtaggg actagtattt cctctatgat tttccatgta acacctttga 600atatacaata ataataagaa gccaccaacc tttgaattat tatctgttcc aatatattag 660atgaggggtg tatcggaatt tgacttccga gttgttcttg cgtgtccgta cgctcgtacg 720gtagctcgtt gggttgttgt accagccatc ctgctactgc gcaacgaaat gcattggtca 780tctcaattaa gtccaaagat tgtagtcgta aacatgcagc caataagagc aaggataata 840gtttagccat tgatatgtct tctaaagcta attattactg tattggaccc acctcgtact 900ctcattctct caccacttgt ttcggaatct gtactgctac aaccagctct tagtcgactg 960ataattaact acccgctttt tctcttctct cccctccaac tgcaaaaatc taatgtggca 1020aaccatttag cctgcttaca tcgtcaaaaa tctaatgtgg taaagtgtga agtgtcctaa 1080agttttagtc cttaatttct ttcaataaac taaactaaac tttagaaaac tcaaacaagt 1140cctcatgttt gcacatttta ggtctcgttt ggtttgaggg actaaagatt agtccctcca 1200ttttagtccc atttagttac taaattacca aacagtagga ctaaaacagg gactaaattg 1260ttttagtccc tagtccctta agatggctaa aagggactaa accatattaa ttccacattt 1320gcccctcatt tagttcaatt gtactaatag caggagaatg ttaaaagtca ttttaatctt 1380cttatgagtc atttaggccc tgtttggttc cattagtcat agaactaaag tttagttgta 1440gggactaaat agattctaaa tacattaaat gcaacacata aagaccaaaa tgcccttttt 1500tgtttgacac cccttttcta gcaagggtat ttggagtaaa tgttgccctt tggtcccttt 1560tagcacccat gtgagggact agagactaaa accaattagt ccctacttta gtcattccgt 1620ttagcaaaat agagactaaa cgagactaaa aacgagaggc taaagattag tctctctaac 1680caaacgggac ctaaaattac tatctgtatg tatctgttgg atggaaaagt cagaacgtcg 1740tggggaccac cacgctacca catggtacgg taatgtcaga aagtcgctat cttcttcgat 1800ctgcatctcc actccagcca gcgctgctta tcatcagcat tcacgaagcc gcccaacgat 1860aataaaaaat gtcagcgcga tcgcgcactg cctataaaac cccggccgtc gcgtccatgg 1920cgtttcagga tccgagcacc agaaagaagc tgagttagct agggtcaaga aagtagtcag 1980cactcagcag gaaaagaagc agagactaca catcatggcg agtgacgccg cgcatggtag 2040ctcgctggac ggggtgacgc cgtcgagcaa gttcgacctg ccggtggact cggagcacaa 2100ggccaagacc atccgcctgc tctccttcgc gaacccgcac atgcgtacct tccacctctc 2160ctggatgtcc ttcttcacct gcgtcgtctc caccttcgcg gcggcgccgc tgatccccat 2220catccgggag aacctgggcc tgaccaaggc cgacatcggc aacgccgggg tggcctccgt 2280ctcgggcgcc atcttctcgc gcctcgccat gggcgccgtc tgcgacctgc tgggcccgcg 2340ctacggctgc gccttcgtcg tcatgctggc ggcgcccgcg gtgttctgca tggccgtcat 2400cgacagcgcc gcgggctacg tcgcgtgccg cttcctcatc ggcttctccc tcgccacctt 2460cgtctcctgc cagtactgga ccagcaccat gttcaacatc aagatcatcg gcaccgtcaa 2520cgcgctggcg tcggggtggg gcgacatggg cggcggcgcc acgcagctca tcatgccctt 2580cgtctacgag gccatcctcc gctgcggcgc cacgccgttc gccgcgtggc gcatcgccta 2640cttcgtgccg gggatcatgc acatcgccgt gggcatcctt gtgctaaccg cggggcagga 2700cctccccgac ggcaacctcc gcagcctccg gaagcagcag cagcagcagc agcagggtga 2760cggcggcgat gccagctgct gccgcaggga cagcttctcc agggtgctct ggcacgccgt 2820cgccaactac cgcacctggg tcttcgtctt cgtgtacggc tacagcatgg gcgtgcagct 2880caccaccaac aacatcatcg ccgagttcta ctacgaccag ttcgagctcg acatccgcgt 2940ggccggcatc atcgccgcct gcttcggcat ggccaacctc gtgtcgcggc ccctgggcgg 3000cgtgctctcc gacctcggcg cgcggtactg gggcatgcgc gcgcgcctct ggaacatctg 3060gatcctccag accgccggcg gcgcgttctg cttctggctc ggccgcgcca gcgagctccc 3120ggcctccgtc accgccatgg tgctcttctc cttctgcgcg caggccgcct gcggcgccac 3180cttcggcgtc atccccttcg tctcccgccg ctcgctgggc gtcatctccg ggctcacggg 3240cgccggcggc aacgtgggcg ccgggctcac gcagctgctc ttcttcacca cgtccagcta 3300ctccacgagg aagggcatcg agaacatggg catcatggcc atggcgtgca cgctgccgct 3360cgtcctcgtg cacttcccgc agtggggttc catgctcctg ccgcccagcg ccgacgccga 3420cgaggagcgg tactatgcct ccgagtggag cgaggacgag aagagcgtag gccgtcacag 3480cgcaagccta aagttcgccg agaacagccg gtccgagcgt ggcaagcgca acgccgtcgc 3540cgtcctcgcc acggccgcgg ccacgccgga gcacgtcgtg taacaactag cgtacgtact 3600tgtaggttct gatcgagcat acagcaaact gtgtaatgta ctctagcagt ctagcttgct 3660ccgatactcc tgcttccaac aaaattatga aacataggct aatatggatc ggtgtacacg 3720tacgtcgtag tatttcctgt gcaacataca caattcagta aatgaacaaa ctttgctcat 3780gtgcattctt ctgcaaagta caaataaaat caaatagaga ggccaggaca acgtctatga 3840tctatcaact tggttgttaa aattaaagaa aaccaactgg agtccaacac ccacaaaaca 3900ttttgtctct aacacgttgt tgtc 3924351569DNAZea mays 35atggcgagtg acgccgcgca tggtagctcg ctggacgggg tgacgccgtc gagcaagttc 60gacctgccgg tggactcgga gcacaaggcc aagaccatcc gcctgctctc cttcgcgaac 120ccgcacatgc gtaccttcca cctctcctgg atgtccttct tcacctgcgt cgtctccacc 180ttcgcggcgg cgccgctgat ccccatcatc cgggagaacc tgggcctgac caaggccgac 240atcggcaacg ccggggtggc ctccgtctcg ggcgccatct tctcgcgcct cgccatgggc 300gccgtctgcg acctgctggg cccgcgctac ggctgcgcct tcgtcgtcat gctggcggcg 360cccgcggtgt tctgcatggc cgtcatcgac agcgccgcgg gctacgtcgc gtgccgcttc 420ctcatcggct tctccctcgc caccttcgtc tcctgccagt actggaccag caccatgttc 480aacatcaaga tcatcggcac cgtcaacgcg ctggcgtcgg ggtggggcga catgggcggc 540ggcgccacgc agctcatcat gcccttcgtc tacgaggcca tcctccgctg cggcgccacg 600ccgttcgccg cgtggcgcat cgcctacttc gtgccgggga tcatgcacat cgccgtgggc 660atccttgtgc taaccgcggg gcaggacctc cccgacggca acctccgcag cctccggaag 720cagcagcagc agcagcagca gggtgacggc ggcgatgcca gctgctgccg cagggacagc 780ttctccaggg tgctctggca cgccgtcgcc aactaccgca cctgggtctt cgtcttcgtg 840tacggctaca gcatgggcgt gcagctcacc accaacaaca tcatcgccga gttctactac 900gaccagttcg agctcgacat ccgcgtggcc ggcatcatcg ccgcctgctt cggcatggcc 960aacctcgtgt cgcggcccct gggcggcgtg ctctccgacc tcggcgcgcg gtactggggc 1020atgcgcgcgc gcctctggaa catctggatc ctccagaccg ccggcggcgc gttctgcttc 1080tggctcggcc gcgccagcga gctcccggcc tccgtcaccg ccatggtgct cttctccttc 1140tgcgcgcagg ccgcctgcgg cgccaccttc ggcgtcatcc ccttcgtctc ccgccgctcg 1200ctgggcgtca tctccgggct cacgggcgcc ggcggcaacg tgggcgccgg gctcacgcag 1260ctgctcttct tcaccacgtc cagctactcc acgaggaagg gcatcgagaa catgggcatc 1320atggccatgg cgtgcacgct gccgctcgtc ctcgtgcact tcccgcagtg gggttccatg 1380ctcctgccgc ccagcgccga cgccgacgag gagcggtact atgcctccga gtggagcgag 1440gacgagaaga gcgtaggccg tcacagcgca agcctaaagt tcgccgagaa cagccggtcc 1500gagcgtggca agcgcaacgc cgtcgccgtc ctcgccacgg ccgcggccac gccggagcac 1560gtcgtgtaa 156936522PRTZea mays 36Met Ala Ser Asp Ala Ala His Gly Ser Ser Leu Asp Gly Val Thr Pro 1 5 10 15 Ser Ser Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys Ala Lys Thr 20 25 30 Ile Arg Leu Leu Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu 35 40 45 Ser Trp Met Ser Phe Phe Thr Cys Val Val Ser Thr Phe Ala Ala Ala 50 55 60 Pro Leu Ile Pro Ile Ile Arg Glu Asn Leu Gly Leu Thr Lys Ala Asp 65 70 75 80 Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ala Ile Phe Ser Arg 85 90 95 Leu Ala Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys 100 105 110 Ala Phe Val Val Met Leu Ala Ala Pro Ala Val Phe Cys Met Ala Val 115 120 125 Ile Asp Ser Ala Ala Gly Tyr Val Ala Cys Arg Phe Leu Ile Gly Phe 130 135 140 Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Thr Ser Thr Met Phe 145 150 155 160 Asn Ile Lys Ile Ile Gly Thr Val Asn Ala Leu Ala Ser Gly Trp Gly 165 170 175 Asp Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Phe Val Tyr Glu 180 185 190 Ala Ile Leu Arg Cys Gly Ala Thr Pro Phe Ala Ala Trp Arg Ile Ala 195 200 205 Tyr Phe Val Pro Gly Ile Met His Ile Ala Val Gly Ile Leu Val Leu 210 215 220 Thr Ala Gly Gln Asp Leu Pro Asp Gly Asn Leu Arg Ser Leu Arg Lys 225 230 235 240 Gln Gln Gln Gln Gln Gln Gln Gly Asp Gly Gly Asp Ala Ser Cys Cys 245 250 255 Arg Arg Asp Ser Phe Ser Arg Val Leu Trp His Ala Val Ala Asn Tyr 260 265 270 Arg Thr Trp Val Phe Val Phe Val Tyr Gly Tyr Ser Met Gly Val Gln 275 280 285 Leu Thr Thr Asn Asn Ile Ile Ala Glu Phe Tyr Tyr Asp Gln Phe Glu 290 295 300 Leu Asp Ile Arg Val Ala Gly Ile Ile Ala Ala Cys Phe Gly Met Ala 305 310 315 320 Asn Leu Val Ser Arg Pro Leu Gly Gly Val Leu Ser Asp Leu Gly Ala 325 330 335 Arg Tyr Trp Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile Leu Gln 340 345 350 Thr Ala Gly Gly Ala Phe Cys Phe Trp Leu Gly Arg Ala Ser Glu Leu 355 360 365 Pro Ala Ser Val Thr Ala Met Val Leu Phe Ser Phe Cys Ala Gln Ala 370 375 380 Ala Cys Gly Ala Thr Phe Gly Val Ile Pro Phe Val Ser Arg Arg Ser 385 390 395 400 Leu Gly Val Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala 405 410 415 Gly Leu Thr Gln Leu Leu Phe Phe Thr Thr Ser Ser Tyr Ser Thr Arg 420 425 430 Lys Gly Ile Glu Asn Met Gly Ile Met Ala Met Ala Cys Thr Leu Pro 435 440 445 Leu Val Leu Val His Phe Pro Gln Trp Gly Ser Met Leu Leu Pro Pro 450 455 460 Ser Ala Asp Ala Asp Glu Glu Arg Tyr Tyr Ala Ser Glu Trp Ser Glu 465 470 475 480 Asp Glu Lys Ser Val Gly Arg His Ser Ala Ser Leu Lys Phe Ala Glu 485 490 495 Asn Ser Arg Ser Glu Arg Gly Lys Arg Asn Ala Val Ala Val Leu Ala 500 505 510 Thr Ala Ala Ala Thr Pro Glu His Val Val 515 520 372014DNAZea mays 37ttcgagggca atgggttcca aagaatgtca tttgaattag acacttagtt atttatgaaa 60aggttttttc tccccgagtt aatttgcttc caaactataa ttaaccctaa gcaaggtgtt 120agttatttgt tttgacggtt tatatatccg tgttagcttg gtggctagct tgtatccatt 180tgacttgacg gcacatgcat gcatgcgtgg agtgcaccgt gcggcggttt gtgacgcggt 240gccaaacgtg caattgactc attgagtagt catcagcagg cttgcgatca ttagacacta 300acaagcatta atatttgctg catatatata tatacacaca catgcttcac tgacgacgct 360tgcaacttga tcttgttaat tattatatat cctaagcaca acgaacaaac cttagatatg 420cgaccatgcc ttgagtagag cgtgaaaaat agggggtgaa aaaaagggac gagtaattat 480agatgacact atttgatatt gtttaaagat gagataggga atgtgctgaa tagatcaatt 540tttaatcagg gatggtaggg actagtattt cctctatgat tttccatgta acacctttga 600atatacaata ataataagaa gccaccaacc tttgaattat tatctgttcc aatatattag 660atgaggggtg tatcggaatt tgacttccga gttgttcttg cgtgtccgta cgctcgtacg 720gtagctcgtt gggttgttgt accagccatc ctgctactgc gcaacgaaat gcattggtca 780tctcaattaa gtccaaagat tgtagtcgta aacatgcagc caataagagc aaggataata 840gtttagccat tgatatgtct tctaaagcta attattactg tattggaccc acctcgtact 900ctcattctct caccacttgt ttcggaatct gtactgctac aaccagctct tagtcgactg 960ataattaact acccgctttt tctcttctct cccctccaac tgcaaaaatc taatgtggca 1020aaccatttag cctgcttaca tcgtcaaaaa tctaatgtgg taaagtgtga agtgtcctaa 1080agttttagtc cttaatttct ttcaataaac taaactaaac tttagaaaac tcaaacaagt 1140cctcatgttt gcacatttta ggtctcgttt ggtttgaggg actaaagatt agtccctcca 1200ttttagtccc atttagttac taaattacca aacagtagga ctaaaacagg gactaaattg 1260ttttagtccc tagtccctta agatggctaa aagggactaa accatattaa ttccacattt 1320gcccctcatt tagttcaatt gtactaatag caggagaatg ttaaaagtca ttttaatctt 1380cttatgagtc atttaggccc tgtttggttc cattagtcat agaactaaag tttagttgta 1440gggactaaat agattctaaa tacattaaat gcaacacata aagaccaaaa tgcccttttt 1500tgtttgacac cccttttcta gcaagggtat ttggagtaaa tgttgccctt tggtcccttt 1560tagcacccat gtgagggact agagactaaa accaattagt ccctacttta gtcattccgt 1620ttagcaaaat agagactaaa cgagactaaa aacgagaggc taaagattag tctctctaac 1680caaacgggac ctaaaattac tatctgtatg tatctgttgg atggaaaagt cagaacgtcg 1740tggggaccac cacgctacca catggtacgg taatgtcaga aagtcgctat cttcttcgat 1800ctgcatctcc actccagcca gcgctgctta tcatcagcat tcacgaagcc gcccaacgat 1860aataaaaaat gtcagcgcga tcgcgcactg cctataaaac cccggccgtc gcgtccatgg 1920cgtttcagga tccgagcacc agaaagaagc tgagttagct agggtcaaga aagtagtcag 1980cactcagcag gaaaagaagc agagactaca catc 2014381014DNAZea mays 38tgcaaaaatc taatgtggca aaccatttag cctgcttaca tcgtcaaaaa tctaatgtgg 60taaagtgtga agtgtcctaa agttttagtc cttaatttct ttcaataaac taaactaaac 120tttagaaaac tcaaacaagt cctcatgttt gcacatttta ggtctcgttt ggtttgaggg 180actaaagatt agtccctcca ttttagtccc atttagttac taaattacca aacagtagga 240ctaaaacagg gactaaattg ttttagtccc tagtccctta agatggctaa aagggactaa 300accatattaa ttccacattt gcccctcatt tagttcaatt gtactaatag caggagaatg 360ttaaaagtca ttttaatctt cttatgagtc atttaggccc tgtttggttc cattagtcat 420agaactaaag tttagttgta gggactaaat agattctaaa tacattaaat gcaacacata 480aagaccaaaa tgcccttttt tgtttgacac cccttttcta gcaagggtat ttggagtaaa 540tgttgccctt tggtcccttt tagcacccat gtgagggact agagactaaa accaattagt 600ccctacttta gtcattccgt ttagcaaaat agagactaaa cgagactaaa aacgagaggc 660taaagattag tctctctaac caaacgggac ctaaaattac tatctgtatg tatctgttgg 720atggaaaagt cagaacgtcg tggggaccac cacgctacca catggtacgg taatgtcaga 780aagtcgctat cttcttcgat ctgcatctcc actccagcca gcgctgctta tcatcagcat 840tcacgaagcc gcccaacgat aataaaaaat gtcagcgcga tcgcgcactg cctataaaac 900cccggccgtc gcgtccatgg cgtttcagga tccgagcacc agaaagaagc tgagttagct 960agggtcaaga aagtagtcag cactcagcag gaaaagaagc agagactaca catc 10143918DNAArtificialPrimer 39cggggttcgc cagcctcc 184017DNAArtificialPrimer 40agtgggctcc ctctccg 174118DNAArtificialPrimer 41gctcgtcatg ccgctcgc 184218DNAArtificialPrimer

42gcactggatg tcgggcat 184320DNAArtificialPrimer 43aattaaccct cactaaaggg 204422DNAArtificialPrimer 44gtaatacgac tcactatagg gc 22455812DNAZea mays 45ggttggcgag cgggtgtggt ctgggcagtg gcaatggcgg gggcagcgaa gaggagggcg 60gtgggggagg gagtggcgag agagggagga aagagagatg aggcgtgtgc aacaacagga 120gacgtacgtc ggcgcttgtc agggtttcgt gcaatgagat atgggtgtgt gggttgattc 180taaagtaatg ttgggagtgt tttgaaaaaa tttgacgcag gacgaccgtt gaaactagtg 240ctttaagtat agtagagatt taaaattaaa gtggacacat ggcccacata ctgaatatta 300aactgcagat attacacttt atcttagcca aaaggtcgag aaatgtatga gttaaaaaag 360gagacatgcc cttttataac tcactcggtc gcttgtccta cttcaactat taagtttgta 420ctattcgaga acgttgtatt acatgtggtt ttgtgtcata ttgggtttgg gtgttttctc 480actaactatc tgggtgrtaa gattgctaga cgagacgtag aggagaaaaa catatctact 540ctacaccgtt tcatgcgtga catgatatac gaaacccaag ttttaaagga gtaaaaataa 600aaataaagat agataaacca taaattacta tctacaaaaa cgtagacagc aggctagata 660ccaaggaggg caagggcaag atggccgagg cacttgtgcc cgccggagct ttggatgcaa 720gatgcaacac actagctgtt cggagacaat cggtgtatca aagaagtaaa aaaatttgga 780tgaaacacac aagctgttac agtggctcta gaggaaagat tgggattttc attttctgat 840gcattcttta cgcagggcaa gagtgttatt tctgctgatg tacacataat tagaagactc 900tctttttttt taattggtgc attttcctta tgaaccacat gcgtaaaaaa ctgggccgaa 960gttcatcacg tcgttgtgcc ctggcacgtc accaatcgca acgctcagct agaagctgct 1020gctgaatgcg caccacagac tcttgggcga aaccagttca tctgtttttt ttttacgcgc 1080agagcggcag agacgacaga gatatgacga tgtatattat ggattaatta aaaagcgatc 1140cggagtttta gatgtctatt tccaccctga ggagccaaaa aggattcatc ggagattcag 1200gaatttctgc atctgcaatc attggaccag agcggcggta gtatattccg atctacaggc 1260ttgcccggcc gagatcctct ggggtcaacc tcgctgctac gcgggagggc gggcgcagcc 1320cctgggcctc acggagagac tccttcacgt ctccgggccc actacagaag gccgagtagt 1380ggcatccgac gctcctgggc ccacttgccg tctcgagtca ccatacgcgc gggcccccag 1440cccacgtaat taaagtgtga ctgggttagt cctgtccgag gctagcgcag agtgggatgc 1500gatgcgacaa aacggccgct agattggatt attagtatag agagtataca gattagagag 1560ttctggaagg ttggttagct catggagttg atcgattccc gctcgtgtca aacacgtata 1620tgttcacctt catatttatc attcgtgtaa attcacggag agtaatatac attgcttact 1680ggagttttgt gtcaaccaat aaccgatcaa agatgttgtt atttactgca tccacactaa 1740taaaacacat aatgtgttct aattttgtct tgggktaatt ttgtcctgga gatgacttta 1800gcttgagggt ggtgttacga cgaaaaacaa tgccgtatag ttctaaggtt agatttttgc 1860aattaatcaa tcacatcgat atgctaatgc taaattgcta atgctatgct ttaaattgct 1920aatgcaatga ggtgatggca ggcagccgca gtcccttttc atggcctcgg ggagccggtg 1980gtaggcacgt acaaaagcca cacggacatg caacgcggcg ccctgcatgc acccgccgcg 2040acaccgcttg ccctccgcct tctcgttctc ggtccaccac cttctattcc atttccacac 2100ccatcaccac acacatttaa aaccaccagc gagtatctaa acctttcacc ccattggtcg 2160cccacaggtc tggaactagt agccactagc tccattctct gcttggctgt ggtagatctc 2220ttcctgcaca gccacgaggc caggcaggca gacgtcacta gctatggtgg cgatggggaa 2280aaagcagcag ctggccgacg acgaagagaa ctgctgctac ggcgtcggca gctctgaggc 2340ggagtgcggc gtcgatgccg agttcagggc gacggatctg cgccctctgt cactgctgtc 2400gccgcacacg caggcgttcc acctcgcctg gctctccctc ttcgcctgct tcttcgcggc 2460ctttgccgcc ccgcccatcc tccctgcgct gcggccggcg ctcgtgctcg cgccctcgga 2520cgcccccgcc gccgcagtgg gctccctctc cgccacgctg gtcggcaggc ttgccatggg 2580gcccgcatgc gacctcctcg gcccgcgccg cgcgtcgggg ttcgccagcc tcctggccgc 2640gctcgccgtc gcggtcaccg cggtcaccgc gtcgtcgccc gcggggttcg tcgcgctgcg 2700cttcgtggcg ggcctctccc tcgccaactt cgtcgccaac cagcactgga tgtcgggcat 2760cttcgcgccc tccgccgtgg ggctcgccaa cgccgtcacg gccggctggg ccaacgtcgg 2820cagcgccgcg gcgcagctcg tcatgccgct cgcgtacgag ctcgtcctcc gcctcggcgt 2880gcccatcacc gtcgcctggc gcgtcaccta cctcctcccc tgcgcgctcc tcatcaccac 2940gggcctcgcc gtcctcgcct tcccytacga cctcccgcgc ggcgccggcg tcggcggcgg 3000agccaagacc ggcaagagct tgtggaaggt ggtgcgcgga ggggtcagca actaccgcgc 3060gtgggtgctc gcgctcacct acggctactg ctacggcgtc gagctcatca tggagaacgt 3120ggccgccgac ttcttccgga aacgtttcca cctccccatg gaggctgcgg gcgccgcggc 3180ggcgtgcttc ggcgcgatga acgcggtggc gcggcccgcg ggcgggttgg cgtcggacgc 3240ggtggcgaga ctgttcggca tgcgcgggag gctgtggctt ctctgggccg tgcagaccac 3300cggcgcggca ctgtgcgtgc tggtcggcag gatgggcgca gcggaagcgc cgtcgctggc 3360ggccaccatg gcggtcatgg tgctgtgcgc cgcgtttgtg caggcctcgt cggggctcac 3420cttcggcatc gtcccgttcg tgtccaagag gtgaatccaa caaacttctt acaacatcta 3480atacagatta ttttgcgtcg gattaattca aaaatagtta tatatagatt ctaagtatat 3540attcacatat agattttttt tccacccaaa aagttataac ttacaaggaa ggacatctat 3600catgcatgtt tcataaacaa attaactaaa gatttttctg tgtttggtta tttagatata 3660aatagatctt gaattatata ttgacgtaca gatcccctcc ctcaaagtta taacgtaaat 3720aataagggca aagacgttga agctgatata tacctctcaa ttgaaagatg gccacgccag 3780ctagcttttt gaagatattt tctaagcaca caaacaccta attactgctc cgttcattta 3840aaattatagc tttaaaaatt aaaatcaaag cgtttaatta gaaaaatcta aaattcttca 3900agctataagt ttaattagaa aaatcaaaac atttaataat ttaaaataga tgaaacatac 3960ccaactaaga gggccacatc gttatcatag gccctaatat agattctata gtagaatcct 4020ggtatactac tattgttgat gttcacctgt tttctgatat ttgtggacga aaataatcag 4080agaggtttcc aacaataaag caactcatta attatttctc tgaacatata ggaggacgtg 4140tttggttgcc acgctagcca tgtccaagct cacgcgcgtg tacttggtta tctgcatgta 4200attaacaaag cgaactcgca cgcacgcgta caacctaagc accttttcca cctcctacat 4260gcatatgtag ggaagcggcc gggtccgcgc gagtcaggag ctctcaactc acaaaccaat 4320cacgtccata acaaccaagg actgtaaaat gtggcgtaca tattttttat gtctaagggc 4380tagtttgaga ctccattatc ctaagagaaa gtgaattaat tagattccta aactagccct 4440gatatgaaaa agaaacaccg gaaaaactac ggtagcaaaa tagccagtgg aaaataaact 4500tgtcgtcaca agttactctt ctattccaat acctcttgta tatgtatttt aaagacacgg 4560ccttaaacat tttttttaaa aaaaaaaaat ccatctaatg aattagccta ggaatatcat 4620gcatggtttt ctcaaaataa tgtcttcgac cccatttggt cacaaattaa tttatctaaa 4680ctagatctaa ctcgtagcat gagttttaga gcgccagagg caatttgtta ttacagaaag 4740attaaggtca tgtttgatac acttcagctt tacaggtgaa ggtgttttaa aaaaaaataa 4800cttcaccaat aacgattgga gaaggaaatg aggaagaaag ctacccaaag ttactttttc 4860ggcttcacct ctgtctaatt ctgcgtctga gcataaaaag gagttttacc tatgaatctt 4920tttgaaaaaa aagaatgttt acaaaaaaat aaatagctca acaacttata aagcttctga 4980ttaatctgta ctaaaaaaga actaactata aacaaaggtc aaagaaacca tgacacattt 5040cttacggctt gtgttgggtc acttaatttc ggtggtgtgt gtgcaggtcg ttgggcgtgg 5100tgtccgggat gacggcgagc ggcggcgcgg tgggcgcgat cgtgacgaac cggctcttct 5160tcagcgggtc gcggtacacc attgaggagg cyatctcgtt gaccggcgcc gccagcctcg 5220tgtgcacgct cccgctggcc ctcgtccact tcccgcgcca cggtggcatg ctctgcggcc 5280caaccgccgt cgtcgatggc gacgatgcag gatacgacaa cgataatagt gctggagatt 5340acacgctcct caaatgaatt gaggaacaaa tgtatgcaac gggggggtcg catgtgaact 5400ttgtacatag cacatccaat ggccttgata gattagcaaa cgattactca tggtttgttt 5460caggatcagg ggtgcgatat gagcgacaca cggatagaaa tatgtcgagt ggcttcgtct 5520gtcgatcacc tgcacataaa tagatagaga gtagagatgg ctcgtaggtt gttcacgtgt 5580cgctgccgca ttggcaattg cgtgtcttat gtttgtgttg gttcgaagag tgagacaata 5640ataagttgtc ggtgttcgaa tcagtaccaa cgagtaaatt gtgtatgcgt gcatgttttg 5700gatttggatg atgtgttcag tgaacgcaag atttatactg attcggatag aacgtcccta 5760cttctagtct tcgatggctc gcgtaatcga taacttcttg ctgaatgctc at 5812462263DNAZea mays 46ggttggcgag cgggtgtggt ctgggcagtg gcaatggcgg gggcagcgaa gaggagggcg 60gtgggggagg gagtggcgag agagggagga aagagagatg aggcgtgtgc aacaacagga 120gacgtacgtc ggcgcttgtc agggtttcgt gcaatgagat atgggtgtgt gggttgattc 180taaagtaatg ttgggagtgt tttgaaaaaa tttgacgcag gacgaccgtt gaaactagtg 240ctttaagtat agtagagatt taaaattaaa gtggacacat ggcccacata ctgaatatta 300aactgcagat attacacttt atcttagcca aaaggtcgag aaatgtatga gttaaaaaag 360gagacatgcc cttttataac tcactcggtc gcttgtccta cttcaactat taagtttgta 420ctattcgaga acgttgtatt acatgtggtt ttgtgtcata ttgggtttgg gtgttttctc 480actaactatc tgggtgrtaa gattgctaga cgagacgtag aggagaaaaa catatctact 540ctacaccgtt tcatgcgtga catgatatac gaaacccaag ttttaaagga gtaaaaataa 600aaataaagat agataaacca taaattacta tctacaaaaa cgtagacagc aggctagata 660ccaaggaggg caagggcaag atggccgagg cacttgtgcc cgccggagct ttggatgcaa 720gatgcaacac actagctgtt cggagacaat cggtgtatca aagaagtaaa aaaatttgga 780tgaaacacac aagctgttac agtggctcta gaggaaagat tgggattttc attttctgat 840gcattcttta cgcagggcaa gagtgttatt tctgctgatg tacacataat tagaagactc 900tctttttttt taattggtgc attttcctta tgaaccacat gcgtaaaaaa ctgggccgaa 960gttcatcacg tcgttgtgcc ctggcacgtc accaatcgca acgctcagct agaagctgct 1020gctgaatgcg caccacagac tcttgggcga aaccagttca tctgtttttt ttttacgcgc 1080agagcggcag agacgacaga gatatgacga tgtatattat ggattaatta aaaagcgatc 1140cggagtttta gatgtctatt tccaccctga ggagccaaaa aggattcatc ggagattcag 1200gaatttctgc atctgcaatc attggaccag agcggcggta gtatattccg atctacaggc 1260ttgcccggcc gagatcctct ggggtcaacc tcgctgctac gcgggagggc gggcgcagcc 1320cctgggcctc acggagagac tccttcacgt ctccgggccc actacagaag gccgagtagt 1380ggcatccgac gctcctgggc ccacttgccg tctcgagtca ccatacgcgc gggcccccag 1440cccacgtaat taaagtgtga ctgggttagt cctgtccgag gctagcgcag agtgggatgc 1500gatgcgacaa aacggccgct agattggatt attagtatag agagtataca gattagagag 1560ttctggaagg ttggttagct catggagttg atcgattccc gctcgtgtca aacacgtata 1620tgttcacctt catatttatc attcgtgtaa attcacggag agtaatatac attgcttact 1680ggagttttgt gtcaaccaat aaccgatcaa agatgttgtt atttactgca tccacactaa 1740taaaacacat aatgtgttct aattttgtct tgggktaatt ttgtcctgga gatgacttta 1800gcttgagggt ggtgttacga cgaaaaacaa tgccgtatag ttctaaggtt agatttttgc 1860aattaatcaa tcacatcgat atgctaatgc taaattgcta atgctatgct ttaaattgct 1920aatgcaatga ggtgatggca ggcagccgca gtcccttttc atggcctcgg ggagccggtg 1980gtaggcacgt acaaaagcca cacggacatg caacgcggcg ccctgcatgc acccgccgcg 2040acaccgcttg ccctccgcct tctcgttctc ggtccaccac cttctattcc atttccacac 2100ccatcaccac acacatttaa aaccaccagc gagtatctaa acctttcacc ccattggtcg 2160cccacaggtc tggaactagt agccactagc tccattctct gcttggctgt ggtagatctc 2220ttcctgcaca gccacgaggc caggcaggca gacgtcacta gct 2263471263DNAZea mays 47acgctcagct agaagctgct gctgaatgcg caccacagac tcttgggcga aaccagttca 60tctgtttttt ttttacgcgc agagcggcag agacgacaga gatatgacga tgtatattat 120ggattaatta aaaagcgatc cggagtttta gatgtctatt tccaccctga ggagccaaaa 180aggattcatc ggagattcag gaatttctgc atctgcaatc attggaccag agcggcggta 240gtatattccg atctacaggc ttgcccggcc gagatcctct ggggtcaacc tcgctgctac 300gcgggagggc gggcgcagcc cctgggcctc acggagagac tccttcacgt ctccgggccc 360actacagaag gccgagtagt ggcatccgac gctcctgggc ccacttgccg tctcgagtca 420ccatacgcgc gggcccccag cccacgtaat taaagtgtga ctgggttagt cctgtccgag 480gctagcgcag agtgggatgc gatgcgacaa aacggccgct agattggatt attagtatag 540agagtataca gattagagag ttctggaagg ttggttagct catggagttg atcgattccc 600gctcgtgtca aacacgtata tgttcacctt catatttatc attcgtgtaa attcacggag 660agtaatatac attgcttact ggagttttgt gtcaaccaat aaccgatcaa agatgttgtt 720atttactgca tccacactaa taaaacacat aatgtgttct aattttgtct tgggktaatt 780ttgtcctgga gatgacttta gcttgagggt ggtgttacga cgaaaaacaa tgccgtatag 840ttctaaggtt agatttttgc aattaatcaa tcacatcgat atgctaatgc taaattgcta 900atgctatgct ttaaattgct aatgcaatga ggtgatggca ggcagccgca gtcccttttc 960atggcctcgg ggagccggtg gtaggcacgt acaaaagcca cacggacatg caacgcggcg 1020ccctgcatgc acccgccgcg acaccgcttg ccctccgcct tctcgttctc ggtccaccac 1080cttctattcc atttccacac ccatcaccac acacatttaa aaccaccagc gagtatctaa 1140acctttcacc ccattggtcg cccacaggtc tggaactagt agccactagc tccattctct 1200gcttggctgt ggtagatctc ttcctgcaca gccacgaggc caggcaggca gacgtcacta 1260gct 1263481455DNAZea mays 48atggtggcga tggggaaaaa gcagcagctg gccgacgacg aagagaactg ctgctacggc 60gtcggcagct ctgaggcgga gtgcggcgtc gatgccgagt tcagggcgac ggatctgcgc 120cctctgtcac tgctgtcgcc gcacacgcag gcgttccacc tcgcctggct ctccctcttc 180gcctgcttct tcgcggcctt tgccgccccg cccatcctcc ctgcgctgcg gccggcgctc 240gtgctcgcgc cctcggacgc ccccgccgcc gcagtgggct ccctctccgc cacgctggtc 300ggcaggcttg ccatggggcc cgcatgcgac ctcctcggcc cgcgccgcgc gtcggggttc 360gccagcctcc tggccgcgct cgccgtcgcg gtcaccgcgg tcaccgcgtc gtcgcccgcg 420gggttcgtcg cgctgcgctt cgtggcgggc ctctccctcg ccaacttcgt cgccaaccag 480cactggatgt cgggcatctt cgcgccctcc gccgtggggc tcgccaacgc cgtcacggcc 540ggctgggcca acgtcggcag cgccgcggcg cagctcgtca tgccgctcgc gtacgagctc 600gtcctccgcc tcggcgtgcc catcaccgtc gcctggcgcg tcacctacct cctcccctgc 660gcgctcctca tcaccacggg cctcgccgtc ctcgccttcc cytacgacct cccgcgcggc 720gccggcgtcg gcggcggagc caagaccggc aagagcttgt ggaaggtggt gcgcggaggg 780gtcagcaact accgcgcgtg ggtgctcgcg ctcacctacg gctactgcta cggcgtcgag 840ctcatcatgg agaacgtggc cgccgacttc ttccggaaac gtttccacct ccccatggag 900gctgcgggcg ccgcggcggc gtgcttcggc gcgatgaacg cggtggcgcg gcccgcgggc 960gggttggcgt cggacgcggt ggcgagactg ttcggcatgc gcgggaggct gtggcttctc 1020tgggccgtgc agaccaccgg cgcggcactg tgcgtgctgg tcggcaggat gggcgcagcg 1080gaagcgccgt cgctggcggc caccatggcg gtcatggtgc tgtgcgccgc gtttgtgcag 1140gcctcgtcgg ggctcacctt cggcatcgtc ccgttcgtgt ccaagaggtc gttgggcgtg 1200gtgtccggga tgacggcgag cggcggcgcg gtgggcgcga tcgtgacgaa ccggctcttc 1260ttcagcgggt cgcggtacac cattgaggag gcyatctcgt tgaccggcgc cgccagcctc 1320gtgtgcacgc tcccgctggc cctcgtccac ttcccgcgcc acggtggcat gctctgcggc 1380ccaaccgccg tcgtcgatgg cgacgatgca ggatacgaca acgataatag tgctggagat 1440tacacgctcc tcaaa 145549485PRTZea mays 49Met Val Ala Met Gly Lys Lys Gln Gln Leu Ala Asp Asp Glu Glu Asn 1 5 10 15 Cys Cys Tyr Gly Val Gly Ser Ser Glu Ala Glu Cys Gly Val Asp Ala 20 25 30 Glu Phe Arg Ala Thr Asp Leu Arg Pro Leu Ser Leu Leu Ser Pro His 35 40 45 Thr Gln Ala Phe His Leu Ala Trp Leu Ser Leu Phe Ala Cys Phe Phe 50 55 60 Ala Ala Phe Ala Ala Pro Pro Ile Leu Pro Ala Leu Arg Pro Ala Leu 65 70 75 80 Val Leu Ala Pro Ser Asp Ala Pro Ala Ala Ala Val Gly Ser Leu Ser 85 90 95 Ala Thr Leu Val Gly Arg Leu Ala Met Gly Pro Ala Cys Asp Leu Leu 100 105 110 Gly Pro Arg Arg Ala Ser Gly Phe Ala Ser Leu Leu Ala Ala Leu Ala 115 120 125 Val Ala Val Thr Ala Val Thr Ala Ser Ser Pro Ala Gly Phe Val Ala 130 135 140 Leu Arg Phe Val Ala Gly Leu Ser Leu Ala Asn Phe Val Ala Asn Gln 145 150 155 160 His Trp Met Ser Gly Ile Phe Ala Pro Ser Ala Val Gly Leu Ala Asn 165 170 175 Ala Val Thr Ala Gly Trp Ala Asn Val Gly Ser Ala Ala Ala Gln Leu 180 185 190 Val Met Pro Leu Ala Tyr Glu Leu Val Leu Arg Leu Gly Val Pro Ile 195 200 205 Thr Val Ala Trp Arg Val Thr Tyr Leu Leu Pro Cys Ala Leu Leu Ile 210 215 220 Thr Thr Gly Leu Ala Val Leu Ala Phe Pro Tyr Asp Leu Pro Arg Gly 225 230 235 240 Ala Gly Val Gly Gly Gly Ala Lys Thr Gly Lys Ser Leu Trp Lys Val 245 250 255 Val Arg Gly Gly Val Ser Asn Tyr Arg Ala Trp Val Leu Ala Leu Thr 260 265 270 Tyr Gly Tyr Cys Tyr Gly Val Glu Leu Ile Met Glu Asn Val Ala Ala 275 280 285 Asp Phe Phe Arg Lys Arg Phe His Leu Pro Met Glu Ala Ala Gly Ala 290 295 300 Ala Ala Ala Cys Phe Gly Ala Met Asn Ala Val Ala Arg Pro Ala Gly 305 310 315 320 Gly Leu Ala Ser Asp Ala Val Ala Arg Leu Phe Gly Met Arg Gly Arg 325 330 335 Leu Trp Leu Leu Trp Ala Val Gln Thr Thr Gly Ala Ala Leu Cys Val 340 345 350 Leu Val Gly Arg Met Gly Ala Ala Glu Ala Pro Ser Leu Ala Ala Thr 355 360 365 Met Ala Val Met Val Leu Cys Ala Ala Phe Val Gln Ala Ser Ser Gly 370 375 380 Leu Thr Phe Gly Ile Val Pro Phe Val Ser Lys Arg Ser Leu Gly Val 385 390 395 400 Val Ser Gly Met Thr Ala Ser Gly Gly Ala Val Gly Ala Ile Val Thr 405 410 415 Asn Arg Leu Phe Phe Ser Gly Ser Arg Tyr Thr Ile Glu Glu Ala Ile 420 425 430 Ser Leu Thr Gly Ala Ala Ser Leu Val Cys Thr Leu Pro Leu Ala Leu 435 440 445 Val His Phe Pro Arg His Gly Gly Met Leu Cys Gly Pro Thr Ala Val 450 455 460 Val Asp Gly Asp Asp Ala Gly Tyr Asp Asn Asp Asn Ser Ala Gly Asp 465 470 475 480 Tyr Thr Leu Leu Lys 485 5014PRTZea maizemisc_feature(6)..(6)Xaa can be any naturally occurring amino acid 50Arg Leu Ala Met Gly Xaa Xaa Cys Asp Leu Leu Gly Pro Arg 1 5 10 5128PRTZea maizeDOMAIN(1)..(28)Xaa=any amino acid 51Thr Phe Gly Xaa Xaa Pro Phe Val Ser Xaa Arg Ser Leu Gly Val Xaa Ser Gly 1 5 10 15Xaa Thr Xaa Xaa Gly Gly Xaa Val Gly Ala 20 255211PRTZea maizemisc_feature(6)..(6)Xaa can be any naturally occurring amino acid 52Cys Thr Leu Pro Leu Xaa Leu Val His Phe Pro1 5 10 531561DNAZea mays 53tagctatata cacatgtctg gtctgacgac aatcaaaagg gatcgctagc tcgggctagc 60cttcctatca ctgtcatgac atgtgctctg

cctctgctgg ttgataagcc gtgcgccttc 120tcgctaattc tttcttgtgc tagaggcgag tcaaacaaac gctgcacctc gtagccctta 180atctgcgcta agggtcacat gaccctgttc cctatcgcta gttaccaacg acccattccc 240cctgacagat acttacgacg cgtccgtacg cggcaggcct cggcagttcg gcatcaccag 300caccggcgcc ggcattcgcc ccctgccagc cggttcgcag attcgcaggg cggagtcggc 360cgcagttgcc gcatcccaaa cgcccgggaa cctttggggc ccctctacga gcaaatgaag 420ttgctgcccc tggcttcgta aagctctgac ttttgatcac ttgattggca gtcgtactcc 480tcgctcatag gccgacacgg ccgcaaagtc aactacccgc tccgccatcc ttcaaccccc 540gccacgcgcc tatatatgtt cgcggccatg tccgtactag tcctccaacc cacaagccac 600aaccccgagc tcagatccct cgcctcgtgt cgtgtctccg gtcgacgacg accaacagcc 660agtgtgggcc agacggacac cgccgagcta tagcgcttgg tgatagcaag ggacgaccgg 720cggccggacc ggagcacgta cgtacgtacc gcagcgatgg ctcggcagca aagcgtgcag 780gccttgtgtg tgctggcggc gcttctcttc gccgcctccc tgccgtcgcc ggccgccgcg 840ggggtgcacc tctcctcgct gcccaaagcg ctcgacgtca ccacctccgc caaacccggc 900caagtcctgc acgccggcgt ggactcgctg acggtgacgt ggagcctgaa cgccacggag 960ccggccggcg ccgacgccgg gtacaagggc gtgaaggtga agctgtgcta cgcgccggcg 1020agccagaagg accgcgggtg gcgcaagtcc gaggacgaca tcagcaagga caaggcgtgc 1080cagttcaagg tcaccgagca ggcgtacgcg gcggcggcgc ccggcagctt ccagtacgcc 1140gtcgcccgcg acgtcccctc gggctcctac tacctgcgcg ccttcgccac ggacgcgtcg 1200ggcgccgagg tggcctacgg ccagacggcg cccaccgccg ccttcgacgt cgccggcatc 1260accggcatcc acgcctctct caagatcgcc gccggcgtct tctcggcctt ctccgtcgtc 1320gcgctcgcct tcttcttcgt catcgagacc cgcaagaaga acaagtagaa cgagttgcgg 1380ctgcgcgcca tacatgcata catgtaaatc gtcggcggcg atgagtggct gtcgttgctg 1440attcattggt gcgcgcgact attttggtgt atcatgtaag ttacttttct gcagtgtgtg 1500cgtcaaaatt accaaataat aacttaagtt tctctgctaa aaaaaaaaaa aaaaaaaaaa 1560a 156154612DNAZea mays 54atggctcggc agcaaagcgt gcaggccttg tgtgtgctgg cggcgcttct cttcgccgcc 60tccctgccgt cgccggccgc cgcgggggtg cacctctcct cgctgcccaa agcgctcgac 120gtcaccacct ccgccaaacc cggccaagtc ctgcacgccg gcgtggactc gctgacggtg 180acgtggagcc tgaacgccac ggagccggcc ggcgccgacg ccgggtacaa gggcgtgaag 240gtgaagctgt gctacgcgcc ggcgagccag aaggaccgcg ggtggcgcaa gtccgaggac 300gacatcagca aggacaaggc gtgccagttc aaggtcaccg agcaggcgta cgcggcggcg 360gcgcccggca gcttccagta cgccgtcgcc cgcgacgtcc cctcgggctc ctactacctg 420cgcgccttcg ccacggacgc gtcgggcgcc gaggtggcct acggccagac ggcgcccacc 480gccgccttcg acgtcgccgg catcaccggc atccacgcct ctctcaagat cgccgccggc 540gtcttctcgg ccttctccgt cgtcgcgctc gccttcttct tcgtcatcga gacccgcaag 600aagaacaagt ag 61255203PRTZea mays 55Met Ala Arg Gln Gln Ser Val Gln Ala Leu Cys Val Leu Ala Ala Leu 1 5 10 15 Leu Phe Ala Ala Ser Leu Pro Ser Pro Ala Ala Ala Gly Val His Leu 20 25 30 Ser Ser Leu Pro Lys Ala Leu Asp Val Thr Thr Ser Ala Lys Pro Gly 35 40 45 Gln Val Leu His Ala Gly Val Asp Ser Leu Thr Val Thr Trp Ser Leu 50 55 60 Asn Ala Thr Glu Pro Ala Gly Ala Asp Ala Gly Tyr Lys Gly Val Lys 65 70 75 80 Val Lys Leu Cys Tyr Ala Pro Ala Ser Gln Lys Asp Arg Gly Trp Arg 85 90 95 Lys Ser Glu Asp Asp Ile Ser Lys Asp Lys Ala Cys Gln Phe Lys Val 100 105 110 Thr Glu Gln Ala Tyr Ala Ala Ala Ala Pro Gly Ser Phe Gln Tyr Ala 115 120 125 Val Ala Arg Asp Val Pro Ser Gly Ser Tyr Tyr Leu Arg Ala Phe Ala 130 135 140 Thr Asp Ala Ser Gly Ala Glu Val Ala Tyr Gly Gln Thr Ala Pro Thr 145 150 155 160 Ala Ala Phe Asp Val Ala Gly Ile Thr Gly Ile His Ala Ser Leu Lys 165 170 175 Ile Ala Ala Gly Val Phe Ser Ala Phe Ser Val Val Ala Leu Ala Phe 180 185 190 Phe Phe Val Ile Glu Thr Arg Lys Lys Asn Lys 195 200 56756DNAZea mays 56tagctatata cacatgtctg gtctgacgac aatcaaaagg gatcgctagc tcgggctagc 60cttcctatca ctgtcatgac atgtgctctg cctctgctgg ttgataagcc gtgcgccttc 120tcgctaattc tttcttgtgc tagaggcgag tcaaacaaac gctgcacctc gtagccctta 180atctgcgcta agggtcacat gaccctgttc cctatcgcta gttaccaacg acccattccc 240cctgacagat acttacgacg cgtccgtacg cggcaggcct cggcagttcg gcatcaccag 300caccggcgcc ggcattcgcc ccctgccagc cggttcgcag attcgcaggg cggagtcggc 360cgcagttgcc gcatcccaaa cgcccgggaa cctttggggc ccctctacga gcaaatgaag 420ttgctgcccc tggcttcgta aagctctgac ttttgatcac ttgattggca gtcgtactcc 480tcgctcatag gccgacacgg ccgcaaagtc aactacccgc tccgccatcc ttcaaccccc 540gccacgcgcc tatatatgtt cgcggccatg tccgtactag tcctccaacc cacaagccac 600aaccccgagc tcagatccct cgcctcgtgt cgtgtctccg gtcgacgacg accaacagcc 660agtgtgggcc agacggacac cgccgagcta tagcgcttgg tgatagcaag ggacgaccgg 720cggccggacc ggagcacgta cgtacgtacc gcagcg 75657594DNAZea mays 57atgacgatgg ctcgtcctgg ggcggctttg ccgctgctgc tggtcgtggt cggcgcttgc 60tgcgcgcgcc tggcggcggc agtgcacctc tccgcgctcg gcaggacact catcgtcgag 120gcgtcgccga aggccggaca agtcctgcac gccggcgagg acacgataac cgtgacatgg 180cacctcaacg cgtcggcgtc cagcgtcggg tacaaggcgc tggaggtgac cctctgctac 240gcgccggcga gccaggagga ccgcgggtgg cgcaaggcca acgacgactt gagcaaggac 300aaggcgtgcc agttcaggat cgcccggcat gcatacgccg gcggccaggg gacgctccgg 360tacagggtcg cccgcgacgt ccccaccgcg tcctaccacg tgcgcgccta cgcgctggac 420gcgtccgggg cgccggtggg ctacggccag accgcgcccg cctactactt ccacgtcgcg 480ggcgtctcgg gcgtccacgc gtccctccgg gtcgccgccg ccgtgctctc cgcgttctcc 540atcgccgcgc tcgccttctt tgtcgtcgtc gagaagagga ggaaggacga gtag 59458197PRTZea mays 58Met Thr Met Ala Arg Pro Gly Ala Ala Leu Pro Leu Leu Leu Val Val 1 5 10 15 Val Gly Ala Cys Cys Ala Arg Leu Ala Ala Ala Val His Leu Ser Ala 20 25 30 Leu Gly Arg Thr Leu Ile Val Glu Ala Ser Pro Lys Ala Gly Gln Val 35 40 45 Leu His Ala Gly Glu Asp Thr Ile Thr Val Thr Trp His Leu Asn Ala 50 55 60 Ser Ala Ser Ser Val Gly Tyr Lys Ala Leu Glu Val Thr Leu Cys Tyr 65 70 75 80 Ala Pro Ala Ser Gln Glu Asp Arg Gly Trp Arg Lys Ala Asn Asp Asp 85 90 95 Leu Ser Lys Asp Lys Ala Cys Gln Phe Arg Ile Ala Arg His Ala Tyr 100 105 110 Ala Gly Gly Gln Gly Thr Leu Arg Tyr Arg Val Ala Arg Asp Val Pro 115 120 125 Thr Ala Ser Tyr His Val Arg Ala Tyr Ala Leu Asp Ala Ser Gly Ala 130 135 140 Pro Val Gly Tyr Gly Gln Thr Ala Pro Ala Tyr Tyr Phe His Val Ala 145 150 155 160 Gly Val Ser Gly Val His Ala Ser Leu Arg Val Ala Ala Ala Val Leu 165 170 175 Ser Ala Phe Ser Ile Ala Ala Leu Ala Phe Phe Val Val Val Glu Lys 180 185 190 Arg Arg Lys Asp Glu 195 5930DNAArtificialPrimer 59ggtcgttggt aactagcgat agggaacagg 306027DNAArtificialPrimer 60gtgcagcgtt tgtttgactc gcctcta 276118DNAArtificialPrimer 61caacggacca gctcttgg 186220DNAArtificialPrimer 62tctttgtggg ttgtggaagg 206320DNAArtificialPrimer 63cgagcagatc gtgcaaatag 206422DNAArtificialPrimer 64gggctttgat atgtttagtt gg 22652917DNAZea maysmisc_feature(517)..(517)n is a, c, g, or t 65ttactatagg gcacgcgtgg tcgacggccc tggctggtcc ttgtttgatt tacttccagg 60attacataat ccagcttata tcataatcta ggtatctaga ttacataatc tatctaataa 120tctgtgttgt tgtttaccta ctaacttatt tataagctgg gttatataat cttgaggcca 180aataaacggg ttctaaaatg gtctagggtc cagtgttaag ctaaatcgac attatgtcta 240gtagtgttaa gctaaatcga catttctttg tgggatgggt ccgatgtgtc gtctagtagt 300gttaagctaa agcgacattt ctttgtgggt tgtggaaggt gtccctgctc tctaagttgt 360tagtgttaag ctaaatgtcg ttctttgtgg gttgtggctg ctccctaagt tgttagtgtt 420aagctaaatg tcgttctttg tgggttgtgg aaggtgttcc ttttccttaa attgttagtg 480ttaagctaaa tcgacatttc tttgtgggtt gtggaangtg ttccttttcc ttaagttgtt 540agttgtgcaa ggtgttcctt atagcatctc ccacatgagc cataatggan tttattttga 600aatataggac tctaaccaac aaaaacatac tccaataggg attctatttt acaaaaaaat 660atcaaatgat tatagggtcg attcttcggg tcctaaatat agtatctaat ataatggagc 720tctatcctca ttttatatat tatttctaaa tttttattta ctaaataaca tgtaacatga 780tttatttcct aatactatga tatagggctc aactgttgga gctgcaaacg ttttttggca 840ataaatactt taaattaggt cctattttaa tttgaaagac tatatcatgc tcttagcgag 900tgtttgtgca tgattgctat ttaggtagtt cagttggggg ctttgatatg tttagttgga 960attctagtat ttttttttgg ttctccgctc ttttgactat cacaacgatc gctatgcgcg 1020agcagactat ttgatctatt aaattatgat ccaaccatgt cacattaagc acttaaactc 1080tttcaccatc agtccaagta tctttataaa aaaccctaac aaaccacaat tgcatatgtg 1140gttagattat aatttaacgt atcagatggt tcgcttgcac tcttacacac ctagaaactg 1200cttgcataac agtcgttctc tttgttatat aatgctttag taatcatgag ctaagggtaa 1260acaaatggta catacaagta gtgaacacat cctcgctacc tatctatagg ggtggaacta 1320gacatcctat tttttagaac aaatttcata ttttaaaata gatatgcttg aaaatttatg 1380ctaatttttt tatagtatca agcatgttat tacacataag aataaaattt tgtataaatt 1440tttatccatt atttgctccc tacaattaaa aaggtgagaa agcaaaaagg tgaagaaaca 1500accgaacccg tatccgtttc atattcaaat ttttacatct attatttgag aatatatttg 1560aaaaatttga ggtttagttt ttacaaatct ttacaaggtt aatgttaaat tataagactg 1620tggatttaca tggtaaattc tatgtcttat ttgtctgcga tcgaagaaaa atgacaaaaa 1680atctgacatt cgaataaaca tttgtttcca ctcctaccta tctcacctcc tatttcaaac 1740tccacttcgt aatacgatac aaaatcaccc cctatctatc tcacctccta tttcaaactc 1800cactcagtaa acaatattgt ctatggtaca aaatcaagtg ttttgtacat ctatttgcac 1860gatctgctcg attcaggcat ccttgacaca caacatactc cttagggcta taaatgtcca 1920aatagagcag acctaatgga tggaccgtgg catgacacga cttatcccaa cacagcacag 1980tccgcccgat tggtcatggg gtctgggttg gtctagcctg atcatcgggt cactcttggg 2040ccacaggtgc gccacaacag gatagcccaa cctatcctat tttttcatgc atatatctat 2100attatagtta gtataaagta aaaaaacaaa aagtatgtgt gttatgttgg ctagatgtgt 2160ttaaataact ctttaaagct agcaactatg gtttaaatca tacatataca catttttagt 2220tttttttatt taaacaatat gagccttata ggcacgtcga gtgtgacggg ccagtgagat 2280gacacattat aattactgat ctagcaggcc gtatctaggt ctttctcgcg gacctttctc 2340gcggaccaag agctggtccg ttggctaatc tatacggtac cgatactgtc ctaattcata 2400ctgggcctag ccgtgtctgt gactgggcat ggctagcgaa gcccgcccat ttgaacacct 2460gtacaagagg ggaatttata aatgaggagg aatgtactca tgcggtacac caggggaatt 2520gttttgttgt gctcagcgat agatttcaac gcaacggtga gccagtttca ccaaaaaaaa 2580gggggaaaag gccacatcaa aggcgaggtg cagacgagca gaagatgcta gcagtgcagc 2640taagtccagc agctagcaat gaaagggtac tcaggattta acaatgccta gagacggcat 2700catcccctca atgatccggt gctctctttt tgtttattca cccgttggcg taactatata 2760cacatgtctg gtctgacgaa cgaatcaagg gatcgctagc tcgggcgagc cttcctatca 2820ctgtcatgac atgtgctctg cctctgctgg ttgataagcc gtgcgccttc tcgctaattc 2880tttcttgtgc tagaggcgag tcaaacaaac gctgcac 2917664498DNAZea maysmisc_feature(517)..(517)n is a, c, g, or t 66ttactatagg gcacgcgtgg tcgacggccc tggctggtcc ttgtttgatt tacttccagg 60attacataat ccagcttata tcataatcta ggtatctaga ttacataatc tatctaataa 120tctgtgttgt tgtttaccta ctaacttatt tataagctgg gttatataat cttgaggcca 180aataaacggg ttctaaaatg gtctagggtc cagtgttaag ctaaatcgac attatgtcta 240gtagtgttaa gctaaatcga catttctttg tgggatgggt ccgatgtgtc gtctagtagt 300gttaagctaa agcgacattt ctttgtgggt tgtggaaggt gtccctgctc tctaagttgt 360tagtgttaag ctaaatgtcg ttctttgtgg gttgtggctg ctccctaagt tgttagtgtt 420aagctaaatg tcgttctttg tgggttgtgg aaggtgttcc ttttccttaa attgttagtg 480ttaagctaaa tcgacatttc tttgtgggtt gtggaangtg ttccttttcc ttaagttgtt 540agttgtgcaa ggtgttcctt atagcatctc ccacatgagc cataatggan tttattttga 600aatataggac tctaaccaac aaaaacatac tccaataggg attctatttt acaaaaaaat 660atcaaatgat tatagggtcg attcttcggg tcctaaatat agtatctaat ataatggagc 720tctatcctca ttttatatat tatttctaaa tttttattta ctaaataaca tgtaacatga 780tttatttcct aatactatga tatagggctc aactgttgga gctgcaaacg ttttttggca 840ataaatactt taaattaggt cctattttaa tttgaaagac tatatcatgc tcttagcgag 900tgtttgtgca tgattgctat ttaggtagtt cagttggggg ctttgatatg tttagttgga 960attctagtat ttttttttgg ttctccgctc ttttgactat cacaacgatc gctatgcgcg 1020agcagactat ttgatctatt aaattatgat ccaaccatgt cacattaagc acttaaactc 1080tttcaccatc agtccaagta tctttataaa aaaccctaac aaaccacaat tgcatatgtg 1140gttagattat aatttaacgt atcagatggt tcgcttgcac tcttacacac ctagaaactg 1200cttgcataac agtcgttctc tttgttatat aatgctttag taatcatgag ctaagggtaa 1260acaaatggta catacaagta gtgaacacat cctcgctacc tatctatagg ggtggaacta 1320gacatcctat tttttagaac aaatttcata ttttaaaata gatatgcttg aaaatttatg 1380ctaatttttt tatagtatca agcatgttat tacacataag aataaaattt tgtataaatt 1440tttatccatt atttgctccc tacaattaaa aaggtgagaa agcaaaaagg tgaagaaaca 1500accgaacccg tatccgtttc atattcaaat ttttacatct attatttgag aatatatttg 1560aaaaatttga ggtttagttt ttacaaatct ttacaaggtt aatgttaaat tataagactg 1620tggatttaca tggtaaattc tatgtcttat ttgtctgcga tcgaagaaaa atgacaaaaa 1680atctgacatt cgaataaaca tttgtttcca ctcctaccta tctcacctcc tatttcaaac 1740tccacttcgt aatacgatac aaaatcaccc cctatctatc tcacctccta tttcaaactc 1800cactcagtaa acaatattgt ctatggtaca aaatcaagtg ttttgtacat ctatttgcac 1860gatctgctcg attcaggcat ccttgacaca caacatactc cttagggcta taaatgtcca 1920aatagagcag acctaatgga tggaccgtgg catgacacga cttatcccaa cacagcacag 1980tccgcccgat tggtcatggg gtctgggttg gtctagcctg atcatcgggt cactcttggg 2040ccacaggtgc gccacaacag gatagcccaa cctatcctat tttttcatgc atatatctat 2100attatagtta gtataaagta aaaaaacaaa aagtatgtgt gttatgttgg ctagatgtgt 2160ttaaataact ctttaaagct agcaactatg gtttaaatca tacatataca catttttagt 2220tttttttatt taaacaatat gagccttata ggcacgtcga gtgtgacggg ccagtgagat 2280gacacattat aattactgat ctagcaggcc gtatctaggt ctttctcgcg gacctttctc 2340gcggaccaag agctggtccg ttggctaatc tatacggtac cgatactgtc ctaattcata 2400ctgggcctag ccgtgtctgt gactgggcat ggctagcgaa gcccgcccat ttgaacacct 2460gtacaagagg ggaatttata aatgaggagg aatgtactca tgcggtacac caggggaatt 2520gttttgttgt gctcagcgat agatttcaac gcaacggtga gccagtttca ccaaaaaaaa 2580gggggaaaag gccacatcaa aggcgaggtg cagacgagca gaagatgcta gcagtgcagc 2640taagtccagc agctagcaat gaaagggtac tcaggattta acaatgccta gagacggcat 2700catcccctca atgatccggt gctctctttt tgtttattca cccgttggcg taactatata 2760cacatgtctg gtctgacgaa cgaatcaagg gatcgctagc tcgggcgagc cttcctatca 2820ctgtcatgac atgtgctctg cctctgctgg ttgataagcc gtgcgccttc tcgctaattc 2880tttcttgtgc tagaggcgag tcaaacaaac gctgcacctc gtagccctta atctgcgcta 2940agggtcacat gaccctgttc cctatcgcta gttaccaacg acccattccc cctgacagat 3000acttacgacg cgtccgtacg cggcaggcct cggcagttcg gcatcaccag caccggcgcc 3060ggcattcgcc ccctgccagc cggttcgcag attcgcaggg cggagtcggc cgcagttgcc 3120gcatcccaaa cgcccgggaa cctttggggc ccctctacga gcaaatgaag ttgctgcccc 3180tggcttcgta aagctctgac ttttgatcac ttgattggca gtcgtactcc tcgctcatag 3240gccgacacgg ccgcaaagtc aactacccgc tccgccatcc ttcaaccccc gccacgcgcc 3300tatatatgtt cgcggccatg tccgtactag tcctccaacc cacaagccac aaccccgagc 3360tcagatccct cgcctcgtgt cgtgtctccg gtcgacgacg accaacagcc agtgtgggcc 3420agacggacac cgccgagcta tagcgcttgg tgatagcaag ggacgaccgg cggccggacc 3480ggagcacgta cgtacgtacc gcagcgatgg ctcggcagca aagcgtgcag gccttgtgtg 3540tgctggcggc gcttctcttc gccgcctccc tgccgtcgcc ggccgccgcg ggggtgcacc 3600tctcctcgct gcccaaagcg ctcgacgtca ccacctccgc caaacccggc caaggtgcgc 3660gcgcgttccg gcccggctca tagtcatagc caaaggatta gcactttgat tacttgctcg 3720gttaattcat agtcctattc ttctctatgt ttgaaacccc cctttagatt tgttcattca 3780caatcaagga gctagctgat taaaatacac acgattgcca taaaatatat gcttctcgca 3840gtcctgcacg ccggcgtgga ctcgctgacg gtgacgtgga gcctgaacgc cacggagccg 3900gccggcgccg acgccgggta caagggcgtg aaggtgaagc tgtgctacgc gccggcgagc 3960cagaaggacc gcgggtggcg caagtccgag gacgacatca gcaaggacaa ggcgtgccag 4020ttcaaggtca ccgagcaggc gtacgcggcg gcggcgcccg gcagcttcca gtacgccgtc 4080gcccgcgacg tcccctcggg ctcctactac ctgcgcgcct tcgccacgga cgcgtcgggc 4140gccgaggtgg cctacggcca gacggcgccc accgccgcct tcgacgtcgc cggcatcacc 4200ggcatccacg cctctctcaa gatcgccgcc ggcgtcttct cggccttctc cgtcgtcgcg 4260ctcgccttct tcttcgtcat cgagacccgc aagaagaaca agtagaacga gttgcggctg 4320cgcgccatac atgcatacat gtaaatcgtc ggcggcgatg agtggctgtc gttgctgatt 4380cattggtgcg cgcgactatt ttggtgtatc atgtaagtta cttttctgca gtgtgtgcgt 4440caaaattacc aaataataac ttaagtttct ctgctaaaaa aaaaaaaaaa aaaaaaaa 4498673506DNAZea maysmisc_feature(517)..(517)n is a, c, g, or t 67ttactatagg gcacgcgtgg tcgacggccc tggctggtcc ttgtttgatt tacttccagg 60attacataat ccagcttata tcataatcta ggtatctaga ttacataatc tatctaataa 120tctgtgttgt tgtttaccta ctaacttatt tataagctgg gttatataat cttgaggcca 180aataaacggg ttctaaaatg gtctagggtc cagtgttaag ctaaatcgac attatgtcta 240gtagtgttaa gctaaatcga catttctttg tgggatgggt ccgatgtgtc gtctagtagt 300gttaagctaa agcgacattt ctttgtgggt tgtggaaggt gtccctgctc tctaagttgt 360tagtgttaag ctaaatgtcg ttctttgtgg gttgtggctg ctccctaagt tgttagtgtt 420aagctaaatg tcgttctttg tgggttgtgg aaggtgttcc ttttccttaa attgttagtg 480ttaagctaaa tcgacatttc tttgtgggtt gtggaangtg ttccttttcc ttaagttgtt 540agttgtgcaa ggtgttcctt atagcatctc ccacatgagc cataatggan tttattttga 600aatataggac tctaaccaac aaaaacatac tccaataggg attctatttt acaaaaaaat 660atcaaatgat tatagggtcg

attcttcggg tcctaaatat agtatctaat ataatggagc 720tctatcctca ttttatatat tatttctaaa tttttattta ctaaataaca tgtaacatga 780tttatttcct aatactatga tatagggctc aactgttgga gctgcaaacg ttttttggca 840ataaatactt taaattaggt cctattttaa tttgaaagac tatatcatgc tcttagcgag 900tgtttgtgca tgattgctat ttaggtagtt cagttggggg ctttgatatg tttagttgga 960attctagtat ttttttttgg ttctccgctc ttttgactat cacaacgatc gctatgcgcg 1020agcagactat ttgatctatt aaattatgat ccaaccatgt cacattaagc acttaaactc 1080tttcaccatc agtccaagta tctttataaa aaaccctaac aaaccacaat tgcatatgtg 1140gttagattat aatttaacgt atcagatggt tcgcttgcac tcttacacac ctagaaactg 1200cttgcataac agtcgttctc tttgttatat aatgctttag taatcatgag ctaagggtaa 1260acaaatggta catacaagta gtgaacacat cctcgctacc tatctatagg ggtggaacta 1320gacatcctat tttttagaac aaatttcata ttttaaaata gatatgcttg aaaatttatg 1380ctaatttttt tatagtatca agcatgttat tacacataag aataaaattt tgtataaatt 1440tttatccatt atttgctccc tacaattaaa aaggtgagaa agcaaaaagg tgaagaaaca 1500accgaacccg tatccgtttc atattcaaat ttttacatct attatttgag aatatatttg 1560aaaaatttga ggtttagttt ttacaaatct ttacaaggtt aatgttaaat tataagactg 1620tggatttaca tggtaaattc tatgtcttat ttgtctgcga tcgaagaaaa atgacaaaaa 1680atctgacatt cgaataaaca tttgtttcca ctcctaccta tctcacctcc tatttcaaac 1740tccacttcgt aatacgatac aaaatcaccc cctatctatc tcacctccta tttcaaactc 1800cactcagtaa acaatattgt ctatggtaca aaatcaagtg ttttgtacat ctatttgcac 1860gatctgctcg attcaggcat ccttgacaca caacatactc cttagggcta taaatgtcca 1920aatagagcag acctaatgga tggaccgtgg catgacacga cttatcccaa cacagcacag 1980tccgcccgat tggtcatggg gtctgggttg gtctagcctg atcatcgggt cactcttggg 2040ccacaggtgc gccacaacag gatagcccaa cctatcctat tttttcatgc atatatctat 2100attatagtta gtataaagta aaaaaacaaa aagtatgtgt gttatgttgg ctagatgtgt 2160ttaaataact ctttaaagct agcaactatg gtttaaatca tacatataca catttttagt 2220tttttttatt taaacaatat gagccttata ggcacgtcga gtgtgacggg ccagtgagat 2280gacacattat aattactgat ctagcaggcc gtatctaggt ctttctcgcg gacctttctc 2340gcggaccaag agctggtccg ttggctaatc tatacggtac cgatactgtc ctaattcata 2400ctgggcctag ccgtgtctgt gactgggcat ggctagcgaa gcccgcccat ttgaacacct 2460gtacaagagg ggaatttata aatgaggagg aatgtactca tgcggtacac caggggaatt 2520gttttgttgt gctcagcgat agatttcaac gcaacggtga gccagtttca ccaaaaaaaa 2580gggggaaaag gccacatcaa aggcgaggtg cagacgagca gaagatgcta gcagtgcagc 2640taagtccagc agctagcaat gaaagggtac tcaggattta acaatgccta gagacggcat 2700catcccctca atgatccggt gctctctttt tgtttattca cccgttggcg taactatata 2760cacatgtctg gtctgacgaa cgaatcaagg gatcgctagc tcgggcgagc cttcctatca 2820ctgtcatgac atgtgctctg cctctgctgg ttgataagcc gtgcgccttc tcgctaattc 2880tttcttgtgc tagaggcgag tcaaacaaac gctgcacctc gtagccctta atctgcgcta 2940agggtcacat gaccctgttc cctatcgcta gttaccaacg acccattccc cctgacagat 3000acttacgacg cgtccgtacg cggcaggcct cggcagttcg gcatcaccag caccggcgcc 3060ggcattcgcc ccctgccagc cggttcgcag attcgcaggg cggagtcggc cgcagttgcc 3120gcatcccaaa cgcccgggaa cctttggggc ccctctacga gcaaatgaag ttgctgcccc 3180tggcttcgta aagctctgac ttttgatcac ttgattggca gtcgtactcc tcgctcatag 3240gccgacacgg ccgcaaagtc aactacccgc tccgccatcc ttcaaccccc gccacgcgcc 3300tatatatgtt cgcggccatg tccgtactag tcctccaacc cacaagccac aaccccgagc 3360tcagatccct cgcctcgtgt cgtgtctccg gtcgacgacg accaacagcc agtgtgggcc 3420agacggacac cgccgagcta tagcgcttgg tgatagcaag ggacgaccgg cggccggacc 3480ggagcacgta cgtacgtacc gcagcg 3506681014DNAZea mays 68cacaacgatc gctatgcgcg agcagactat ttgatctatt aaattatgat ccaaccatgt 60cacattaagc acttaaactc tttcaccatc agtccaagta tctttataaa aaaccctaac 120aaaccacaat tgcatatgtg gttagattat aatttaacgt atcagatggt tcgcttgcac 180tcttacacac ctagaaactg cttgcataac agtcgttctc tttgttatat aatgctttag 240taatcatgag ctaagggtaa acaaatggta catacaagta gtgaacacat cctcgctacc 300tatctatagg ggtggaacta gacatcctat tttttagaac aaatttcata ttttaaaata 360gatatgcttg aaaatttatg ctaatttttt tatagtatca agcatgttat tacacataag 420aataaaattt tgtataaatt tttatccatt atttgctccc tacaattaaa aaggtgagaa 480agcaaaaagg tgaagaaaca accgaacccg tatccgtttc atattcaaat ttttacatct 540attatttgag aatatatttg aaaaatttga ggtttagttt ttacaaatct ttacaaggtt 600aatgttaaat tataagactg tggatttaca tggtaaattc tatgtcttat ttgtctgcga 660tcgaagaaaa atgacaaaaa atctgacatt cgaataaaca tttgtttcca ctcctaccta 720tctcacctcc tatttcaaac tccacttcgt aatacgatac aaaatcaccc cctatctatc 780tcacctccta tttcaaactc cactcagtaa acaatattgt ctatggtaca aaatcaagtg 840ttttgtacat ctatttgcac gatctgctcg attcaggcat ccttgacaca caacatactc 900cttagggcta taaatgtcca aatagagcag acctaatgga tggaccgtgg catgacacga 960cttatcccaa cacagcacag tccgcccgat tggtcatggg gtctgggttg gtct 1014691492DNAZea mays 69agcctgatca tcgggtcact cttgggccac aggtgcgcca caacaggata gcccaaccta 60tcctattttt tcatgcatat atctatatta tagttagtat aaagtaaaaa aacaaaaagt 120atgtgtgtta tgttggctag atgtgtttaa ataactcttt aaagctagca actatggttt 180aaatcataca tatacacatt tttagttttt tttatttaaa caatatgagc cttataggca 240cgtcgagtgt gacgggccag tgagatgaca cattataatt actgatctag caggccgtat 300ctaggtcttt ctcgcggacc tttctcgcgg accaagagct ggtccgttgg ctaatctata 360cggtaccgat actgtcctaa ttcatactgg gcctagccgt gtctgtgact gggcatggct 420agcgaagccc gcccatttga acacctgtac aagaggggaa tttataaatg aggaggaatg 480tactcatgcg gtacaccagg ggaattgttt tgttgtgctc agcgatagat ttcaacgcaa 540cggtgagcca gtttcaccaa aaaaaagggg gaaaaggcca catcaaaggc gaggtgcaga 600cgagcagaag atgctagcag tgcagctaag tccagcagct agcaatgaaa gggtactcag 660gatttaacaa tgcctagaga cggcatcatc ccctcaatga tccggtgctc tctttttgtt 720tattcacccg ttggcgtaac tatatacaca tgtctggtct gacgaacgaa tcaagggatc 780gctagctcgg gcgagccttc ctatcactgt catgacatgt gctctgcctc tgctggttga 840taagccgtgc gccttctcgc taattctttc ttgtgctaga ggcgagtcaa acaaacgctg 900cacctcgtag cccttaatct gcgctaaggg tcacatgacc ctgttcccta tcgctagtta 960ccaacgaccc attccccctg acagatactt acgacgcgtc cgtacgcggc aggcctcggc 1020agttcggcat caccagcacc ggcgccggca ttcgccccct gccagccggt tcgcagattc 1080gcagggcgga gtcggccgca gttgccgcat cccaaacgcc cgggaacctt tggggcccct 1140ctacgagcaa atgaagttgc tgcccctggc ttcgtaaagc tctgactttt gatcacttga 1200ttggcagtcg tactcctcgc tcataggccg acacggccgc aaagtcaact acccgctccg 1260ccatccttca acccccgcca cgcgcctata tatgttcgcg gccatgtccg tactagtcct 1320ccaacccaca agccacaacc ccgagctcag atccctcgcc tcgtgtcgtg tctccggtcg 1380acgacgacca acagccagtg tgggccagac ggacaccgcc gagctatagc gcttggtgat 1440agcaagggac gaccggcggc cggaccggag cacgtacgta cgtaccgcag cg 1492703621DNAZea mays 70tggtccttgt ttgatttact tccaggatta tataatccag cttatggatt atataagtac 60ctattgacgt cacgtgctta tgtattataa taatctaggt atatagatta tataatctat 120ctaataataa tctgtgttgt ttgtttatct ctcaaaacaa acaggtccta aaatggtccc 180gggcgtccaa tgtgtcgtca agtagtgtta agctaaatcg acatttcttt gtgggttgtg 240tggaaggtgt tccttttcct taagttgtta gttgtgcaag gtgttcctta gagcatctcc 300aataggacct ataatggatt ctattttgaa ttataagact ctaacaacaa aagcatactt 360taatggggat tctattttac aaaaaaatat caaatgatta tatggtcgat tcctcgggtc 420ctaaatatag tatctcatat aatagagctc tatcctcatt ttatatacta tttttaagtt 480tttatttact aaataacatg atttattttc taatactatg aactcaacta ttagagctgt 540aaacgttttt gtggtactaa acactttaaa tcaggtccta ttttaatttg aaggacttaa 600atataagact tctggttaga gatgctctta gcgagtgttt gtgcatgatt gctatttagt 660ctttgtggat tgtggaaggt gttacttttc ctcaagttgt tagttgtgca aggtgtttct 720tagagcatct ctaacaggag ccttaacgga atctattttg aagtatagta ctttaacacc 780aaaaacatac tttaataggg gtcctatttt acaaaaaaat tatcaaatga ttataaggtc 840cactcctcgg gtcctaaata taatatctca tatactagag ctctatcctc attttatata 900ctatccctag gtttttattc cctaaataac atgatttatt tcctaatact aagatatagg 960gctcaactat tggagttgca aatgtttttt ggcactaaac actttatatc aggtcctatt 1020ttaattttaa tttgaaggac tcaaatatag gacttctcgt tagagatgct cttagcgagt 1080gtttgtgcat gattgctatt tatgtctgta gtttagttgg gggctttaat atgtttagtt 1140gaagttctag tattttttag gttctccact ctttggatta tgacaacgac cactatccaa 1200gcagtctttg agtgcaaacg cgcgagcaaa ctatctgatc tattaaatta tgatccaacc 1260gttatgtcat attgaagact taaacccttt caccaccagc ccaagtatct ttatgaaaaa 1320ccctaacaaa ccacaattgc atctatggtt ggattataat ttaacgtatc agatggttcg 1380cttgcatgct tacatatcta gaaactgttt gcataacagt cgttctcttt ggttatataa 1440tgctttagta atcatcagcc aagtgtaaac aaatggtaca aactagtagt gaacacatcc 1500tccctaccta tctctagggg tgtaactaga tatccgaatt cttagaacaa atttcatatt 1560ttaaaataga tatgcttcaa aatttatgct aatctttttt atattatcaa gcatattatt 1620acacataaga ataaaatttt gtatagaatt ttatccatta tttgttccct agaatttaaa 1680aagtgaaaaa acattcgaat ctgtatcagt ttcgtattca aatttttaca tctattattt 1740gagaatatat atgataaatt tgaggtttag tttttatgaa tctttacaag gttaatgtta 1800aatacatgac tatggattta catagtaaat tctatgtctt atttgtccgc gattgaagaa 1860aaatgacaaa aagatctgac attcgaataa acatctgttt ccactcctac ctatctgacc 1920tcctatttca aactccactt tgtaacacgg tacaaaatca ctccctacct atctgacctc 1980ctatttcaaa ctccactcag taaacaatat tgtctatggt acaaaaccaa gtgttttata 2040catctatttg cacgatctgc tcgagtcagg catccttgac acacaacata ctccttgtgg 2100ctataaatgt ccaaatagag cagacctaat gggtggaccg ttgcatgaca cgacttatcc 2160caagacgagc acagttcgcc ccattggtca tgggggtccg ggctagtcta gcctgatcat 2220cgggtcacac ttaggccaca ggtgtgccac aacgggatag cccaacatgt ccctttttgt 2280catgcatata tctatattat agttagtata atgtaaaaaa acaaaaggta tgtgtgttat 2340gttggttaga tgtgtttaaa taactcttta aagctagcaa ctatggttta aatcatacat 2400atacacattt ttattttatt tttatttaaa cgatatgggc cttctaggca cgtcgagtgt 2460gacgggccag tgagatgaca cattataatt actggtctag caggccgtac ctaggtcttt 2520ctcgtgggcc aagactaagg gttggcccgt tggctaatct gtacggtacc gatactgtcc 2580taattcattt gaacacctgt agaagagggg aatttataat tgaggaggaa tgtactcatg 2640cggtacacca ggggaattgt tttgttgtgc tcagcgatag atttcaacgc aacggtgagc 2700cagtttcact aaaaaaaggg gggggggggg ggggggggga aggccacatc aaaggcgagg 2760tgctgacgag cagaagatgc tagcagtgac gccaagtcca gcagctagca atgaaagggt 2820actcgggatt taacaatgcc tagagacggc atcatcccct caataatccg gtgctctctt 2880tttgtttatt caccagttgg cgtagctata tacacatgtc tggtctgacg aacaaatcaa 2940gggatcgcta gctcgggcta gccttcctat cactgtcatg acatgtgctc tgcctctgct 3000ggttgataag ccgtgcgcct tctcgctaat tctttcttgt gctagaggcg agtcaaacaa 3060acgctgcacc tcgtagccct taatctgcgc taagggtcac atgaccctgt tccctatcgc 3120tagttaccaa cgacccattc cccctgacag atacttacga cgcgtccgta cgcggcaggc 3180ctcggcagtt cggcatcacc agcaccggcg ccggcattcg ccccctgcca gccggttcgc 3240agattcgcag ggcggagtcg gccgcagttg ccgcatccca aacgcccggg aacctttggg 3300gcccctctac gagcaaatga agttgctgcc cctggcttcg taaagctctg acttttgatc 3360acttgattgg cagtcgtact cctcgctcat aggccgacac ggccgcaaag tcaactaccc 3420gctccgccat ccttcaaccc ccgccacgcg cctatatatg ttcgcggcca tgtccgtact 3480agtcctccaa cccacaagcc acaaccccga gctcagatcc ctcgcctcgt gtcgtgtctc 3540cggtcgacga cgaccaacag ccagtgtggg ccagacggac accgccgagc tatagcgctt 3600ggtgatagca agggacgacc g 3621713236DNAZea mays 71tggtccttgt ttgatttact tccaggatta tataatccag cttatggatt atataagtac 60ctattgacgt cacgtgctta tgtattataa taatctaggt atatagatta tataatctat 120ctaataataa tctgtgttgt ttgtttatct ctcaaaacaa acaggtccta aaatggtccc 180gggcgtccaa tgtgtcgtca agtagtgtta agctaaatcg acatttcttt gtgggttgtg 240tggaaggtgt tccttttcct taagttgtta gttgtgcaag gtgttcctta gagcatctcc 300aataggacct ataatggatt ctattttgaa ttataagact ctaacaacaa aagcatactt 360taatggggat tctattttac aaaaaaatat caaatgatta tatggtcgat tcctcgggtc 420ctaaatatag tatctcatat aatagagctc tatcctcatt ttatatacta tttttaagtt 480tttatttact aaataacatg atttattttc taatactatg aactcaacta ttagagctgt 540aaacgttttt gtggtactaa acactttaaa tcaggtccta ttttaatttg aaggacttaa 600atataagact tctggttaga gatgctctta gcgagtgttt gtgcatgatt gctatttagt 660ctttgtggat tgtggaaggt gttacttttc ctcaagttgt tagttgtgca aggtgtttct 720tagagcatct ctaacaggag ccttaacgga atctattttg aagtatagta ctttaacacc 780aaaaacatac tttaataggg gtcctatttt acaaaaaaat tatcaaatga ttataaggtc 840cactcctcgg gtcctaaata taatatctca tatactagag ctctatcctc attttatata 900ctatccctag gtttttattc cctaaataac atgatttatt tcctaatact aagatatagg 960gctcaactat tggagttgca aatgtttttt ggcactaaac actttatatc aggtcctatt 1020ttaattttaa tttgaaggac tcaaatatag gacttctcgt tagagatgct cttagcgagt 1080gtttgtgcat gattgctatt tatgtctgta gtttagttgg gggctttaat atgtttagtt 1140gaagttctag tattttttag gttctccact ctttggatta tgacaacgac cactatccaa 1200gcagtctttg agtgcaaacg cgcgagcaaa ctatctgatc tattaaatta tgatccaacc 1260gttatgtcat attgaagact taaacccttt caccaccagc ccaagtatct ttatgaaaaa 1320ccctaacaaa ccacaattgc atctatggtt ggattataat ttaacgtatc agatggttcg 1380cttgcatgct tacatatcta gaaactgttt gcataacagt cgttctcttt ggttatataa 1440tgctttagta atcatcagcc aagtgtaaac aaatggtaca aactagtagt gaacacatcc 1500tccctaccta tctctagggg tgtaactaga tatccgaatt cttagaacaa atttcatatt 1560ttaaaataga tatgcttcaa aatttatgct aatctttttt atattatcaa gcatattatt 1620acacataaga ataaaatttt gtatagaatt ttatccatta tttgttccct agaatttaaa 1680aagtgaaaaa acattcgaat ctgtatcagt ttcgtattca aatttttaca tctattattt 1740gagaatatat atgataaatt tgaggtttag tttttatgaa tctttacaag gttaatgtta 1800aatacatgac tatggattta catagtaaat tctatgtctt atttgtccgc gattgaagaa 1860aaatgacaaa aagatctgac attcgaataa acatctgttt ccactcctac ctatctgacc 1920tcctatttca aactccactt tgtaacacgg tacaaaatca ctccctacct atctgacctc 1980ctatttcaaa ctccactcag taaacaatat tgtctatggt acaaaaccaa gtgttttata 2040catctatttg cacgatctgc tcgagtcagg catccttgac acacaacata ctccttgtgg 2100ctataaatgt ccaaatagag cagacctaat gggtggaccg ttgcatgaca cgacttatcc 2160caagacgagc acagttcgcc ccattggtca tgggggtccg ggctagtcta gcctgatcat 2220cgggtcacac ttaggccaca ggtgtgccac aacgggatag cccaacatgt ccctttttgt 2280catgcatata tctatattat agttagtata atgtaaaaaa acaaaaggta tgtgtgttat 2340gttggttaga tgtgtttaaa taactcttta aagctagcaa ctatggttta aatcatacat 2400atacacattt ttattttatt tttatttaaa cgatatgggc cttctaggca cgtcgagtgt 2460gacgggccag tgagatgaca cattataatt actggtctag caggccgtac ctaggtcttt 2520ctcgtgggcc aagactaagg gttggcccgt tggctaatct gtacggtacc gatactgtcc 2580taattcattt gaacacctgt agaagagggg aatttataat tgaggaggaa tgtactcatg 2640cggtacacca ggggaattgt tttgttgtgc tcagcgatag atttcaacgc aacggtgagc 2700cagtttcact aaaaaaaggg gggggggggg ggggggggga aggccacatc aaaggcgagg 2760tgctgacgag cagaagatgc tagcagtgac gccaagtcca gcagctagca atgaaagggt 2820actcgggatt taacaatgcc tagagacggc atcatcccct caataatccg gtgctctctt 2880tttgtttatt caccagttgg cgtagctata tacacatgtc tggtctgacg aacaaatcaa 2940gggatcgcta gctcgggcta gccttcctat cactgtcatg acatgtgctc tgcctctgct 3000ggttgataag ccgtgcgcct tctcgctaat tctttcttgt gctagaggcg agtcaaacaa 3060acgctgcacc tcgtagccct taatctgcgc taagggtcac atgaccctgt tccctatcgc 3120tagttaccaa cgacccattc cccctgacag atacttacga cgcgtccgta cgcggcaggc 3180ctcggcagtt cggcatcacc agcaccggcg ccggcattcg ccccctgcca gccggt 3236721000DNAZea mays 72tggtccttgt ttgatttact tccaggatta tataatccag cttatggatt atataagtac 60ctattgacgt cacgtgctta tgtattataa taatctaggt atatagatta tataatctat 120ctaataataa tctgtgttgt ttgtttatct ctcaaaacaa acaggtccta aaatggtccc 180gggcgtccaa tgtgtcgtca agtagtgtta agctaaatcg acatttcttt gtgggttgtg 240tggaaggtgt tccttttcct taagttgtta gttgtgcaag gtgttcctta gagcatctcc 300aataggacct ataatggatt ctattttgaa ttataagact ctaacaacaa aagcatactt 360taatggggat tctattttac aaaaaaatat caaatgatta tatggtcgat tcctcgggtc 420ctaaatatag tatctcatat aatagagctc tatcctcatt ttatatacta tttttaagtt 480tttatttact aaataacatg atttattttc taatactatg aactcaacta ttagagctgt 540aaacgttttt gtggtactaa acactttaaa tcaggtccta ttttaatttg aaggacttaa 600atataagact tctggttaga gatgctctta gcgagtgttt gtgcatgatt gctatttagt 660ctttgtggat tgtggaaggt gttacttttc ctcaagttgt tagttgtgca aggtgtttct 720tagagcatct ctaacaggag ccttaacgga atctattttg aagtatagta ctttaacacc 780aaaaacatac tttaataggg gtcctatttt acaaaaaaat tatcaaatga ttataaggtc 840cactcctcgg gtcctaaata taatatctca tatactagag ctctatcctc attttatata 900ctatccctag gtttttattc cctaaataac atgatttatt tcctaatact aagatatagg 960gctcaactat tggagttgca aatgtttttt ggcactaaac 1000732236DNAZea mays 73actttatatc aggtcctatt ttaattttaa tttgaaggac tcaaatatag gacttctcgt 60tagagatgct cttagcgagt gtttgtgcat gattgctatt tatgtctgta gtttagttgg 120gggctttaat atgtttagtt gaagttctag tattttttag gttctccact ctttggatta 180tgacaacgac cactatccaa gcagtctttg agtgcaaacg cgcgagcaaa ctatctgatc 240tattaaatta tgatccaacc gttatgtcat attgaagact taaacccttt caccaccagc 300ccaagtatct ttatgaaaaa ccctaacaaa ccacaattgc atctatggtt ggattataat 360ttaacgtatc agatggttcg cttgcatgct tacatatcta gaaactgttt gcataacagt 420cgttctcttt ggttatataa tgctttagta atcatcagcc aagtgtaaac aaatggtaca 480aactagtagt gaacacatcc tccctaccta tctctagggg tgtaactaga tatccgaatt 540cttagaacaa atttcatatt ttaaaataga tatgcttcaa aatttatgct aatctttttt 600atattatcaa gcatattatt acacataaga ataaaatttt gtatagaatt ttatccatta 660tttgttccct agaatttaaa aagtgaaaaa acattcgaat ctgtatcagt ttcgtattca 720aatttttaca tctattattt gagaatatat atgataaatt tgaggtttag tttttatgaa 780tctttacaag gttaatgtta aatacatgac tatggattta catagtaaat tctatgtctt 840atttgtccgc gattgaagaa aaatgacaaa aagatctgac attcgaataa acatctgttt 900ccactcctac ctatctgacc tcctatttca aactccactt tgtaacacgg tacaaaatca 960ctccctacct atctgacctc ctatttcaaa ctccactcag taaacaatat tgtctatggt 1020acaaaaccaa gtgttttata catctatttg cacgatctgc tcgagtcagg catccttgac 1080acacaacata ctccttgtgg ctataaatgt ccaaatagag cagacctaat gggtggaccg 1140ttgcatgaca cgacttatcc caagacgagc acagttcgcc ccattggtca tgggggtccg 1200ggctagtcta gcctgatcat cgggtcacac ttaggccaca ggtgtgccac aacgggatag 1260cccaacatgt ccctttttgt catgcatata tctatattat agttagtata atgtaaaaaa 1320acaaaaggta tgtgtgttat gttggttaga tgtgtttaaa taactcttta aagctagcaa 1380ctatggttta aatcatacat atacacattt ttattttatt tttatttaaa cgatatgggc 1440cttctaggca cgtcgagtgt gacgggccag tgagatgaca cattataatt actggtctag 1500caggccgtac ctaggtcttt ctcgtgggcc aagactaagg gttggcccgt tggctaatct 1560gtacggtacc gatactgtcc taattcattt gaacacctgt agaagagggg aatttataat 1620tgaggaggaa tgtactcatg

cggtacacca ggggaattgt tttgttgtgc tcagcgatag 1680atttcaacgc aacggtgagc cagtttcact aaaaaaaggg gggggggggg ggggggggga 1740aggccacatc aaaggcgagg tgctgacgag cagaagatgc tagcagtgac gccaagtcca 1800gcagctagca atgaaagggt actcgggatt taacaatgcc tagagacggc atcatcccct 1860caataatccg gtgctctctt tttgtttatt caccagttgg cgtagctata tacacatgtc 1920tggtctgacg aacaaatcaa gggatcgcta gctcgggcta gccttcctat cactgtcatg 1980acatgtgctc tgcctctgct ggttgataag ccgtgcgcct tctcgctaat tctttcttgt 2040gctagaggcg agtcaaacaa acgctgcacc tcgtagccct taatctgcgc taagggtcac 2100atgaccctgt tccctatcgc tagttaccaa cgacccattc cccctgacag atacttacga 2160cgcgtccgta cgcggcaggc ctcggcagtt cggcatcacc agcaccggcg ccggcattcg 2220ccccctgcca gccggt 2236741237DNAZea Mays 74gtaaacaata ttgtctatgg tacaaaacca agtgttttat acatctattt gcacgatctg 60ctcgagtcag gcatccttga cacacaacat actccttgtg gctataaatg tccaaataga 120gcagacctaa tgggtggacc gttgcatgac acgacttatc ccaagacgag cacagttcgc 180cccattggtc atgggggtcc gggctagtct agcctgatca tcgggtcaca cttaggccac 240aggtgtgcca caacgggata gcccaacatg tccctttttg tcatgcatat atctatatta 300tagttagtat aatgtaaaaa aacaaaaggt atgtgtgtta tgttggttag atgtgtttaa 360ataactcttt aaagctagca actatggttt aaatcataca tatacacatt tttattttat 420ttttatttaa acgatatggg ccttctaggc acgtcgagtg tgacgggcca gtgagatgac 480acattataat tactggtcta gcaggccgta cctaggtctt tctcgtgggc caagactaag 540ggttggcccg ttggctaatc tgtacggtac cgatactgtc ctaattcatt tgaacacctg 600tagaagaggg gaatttataa ttgaggagga atgtactcat gcggtacacc aggggaattg 660ttttgttgtg ctcagcgata gatttcaacg caacggtgag ccagtttcac taaaaaaagg 720gggggggggg gggggggggg aaggccacat caaaggcgag gtgctgacga gcagaagatg 780ctagcagtga cgccaagtcc agcagctagc aatgaaaggg tactcgggat ttaacaatgc 840ctagagacgg catcatcccc tcaataatcc ggtgctctct ttttgtttat tcaccagttg 900gcgtagctat atacacatgt ctggtctgac gaacaaatca agggatcgct agctcgggct 960agccttccta tcactgtcat gacatgtgct ctgcctctgc tggttgataa gccgtgcgcc 1020ttctcgctaa ttctttcttg tgctagaggc gagtcaaaca aacgctgcac ctcgtagccc 1080ttaatctgcg ctaagggtca catgaccctg ttccctatcg ctagttacca acgacccatt 1140ccccctgaca gatacttacg acgcgtccgt acgcggcagg cctcggcagt tcggcatcac 1200cagcaccggc gccggcattc gccccctgcc agccggt 12377521DNAArtificialPrimer 75gccgtgcgcc ttctcgctaa t 217624DNAArtificialPrimer 76gcgaggagta cgactgccaa tcaa 247732DNAArtificialPrimer 77ttcggatcct ggtccttgtt tgatttactt cc 327827DNAArtificialPrimer 78ggcaagcttc ggtcgtccct tgctatc 277918DNAArtificialPrimer 79tgtaaaacga cggccagt 188019DNAArtificialPrimer 80ggaaacagct atgaccatg 198124DNAArtificialPrimer 81tcaaatgatt atatggtcga ttcc 248220DNAArtificialPrimer 82cgagcagatc gtgcaaatag 208318DNAArtificialPrimer 83tgctagctgc tggacttg 188424DNAArtificialPrimer 84ttgattggca gtcgtactcc tcgc 24852777DNAArtificial SeqeunceVector 85gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact 60ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt 120cttaagctcg ggcccgcgtt aacgctacca tggagctcca aataatgatt ttattttgac 180tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt 240tgtatagaaa agttgggccg aattcgagct cggtacggcc agaatggccc ggaccgggtt 300accgaattcg agctcggtac cctgggatcc gatatcgatg ggccctggcc gaagcttggt 360cacccggtcc gggcctagaa ggccagcttc aagtttgtac aaaaaagttg aacgagaaac 420gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata 480atactgtaaa acacaacata tgcagtcact atgaatcaac tacttagatg gtattagtga 540cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt 600attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt 660acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 720gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 780ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 840gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 900gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 960aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 1020tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag 1080gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt 1140gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 1200ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 1260aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 1320gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 1380ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 1440gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 1500atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta 1560attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac 1620caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga 1680tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 1740aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 1800aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 1860taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 1920taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 1980agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 2040tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca 2100cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2160agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2220gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2280aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 2340tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 2400ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 2460aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 2520ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta 2580ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg 2640cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg 2700cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga 2760caaacaacag ataaaac 2777866377DNAArtificial SeqeunceVector 86gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact 60ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt 120cttaagctcg ggcccgcgtt aacgctacca tggagctcca aataatgatt ttattttgac 180tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata atgccaactt 240tgtatagaaa agttgggccg aattcgagct cggtacggcc agaatggccc ggaccgggtt 300accgaattcg agctcggtac cctgggatcc gcaagggacg accgtggtcc ttgtttgatt 360tacttccagg attatataat ccagcttatg gattatataa gtacctattg acgtcacgtg 420cttatgtatt ataataatct aggtatatag attatataat ctatctaata ataatctgtg 480ttgtttgttt atctctcaaa acaaacaggt cctaaaatgg tcccgggcgt ccaatgtgtc 540gtcaagtagt gttaagctaa atcgacattt ctttgtgggt tgtgtggaag gtgttccttt 600tccttaagtt gttagttgtg caaggtgttc cttagagcat ctccaatagg acctataatg 660gattctattt tgaattataa gactctaaca acaaaagcat actttaatgg ggattctatt 720ttacaaaaaa atatcaaatg attatatggt cgattcctcg ggtcctaaat atagtatctc 780atataataga gctctatcct cattttatat actattttta agtttttatt tactaaataa 840catgatttat tttctaatac tatgaactca actattagag ctgtaaacgt ttttgtggta 900ctaaacactt taaatcaggt cctattttaa tttgaaggac ttaaatataa gacttctggt 960tagagatgct cttagcgagt gtttgtgcat gattgctatt tagtctttgt ggattgtgga 1020aggtgttact tttcctcaag ttgttagttg tgcaaggtgt ttcttagagc atctctaaca 1080ggagccttaa cggaatctat tttgaagtat agtactttaa caccaaaaac atactttaat 1140aggggtccta ttttacaaaa aaattatcaa atgattataa ggtccactcc tcgggtccta 1200aatataatat ctcatatact agagctctat cctcatttta tatactatcc ctaggttttt 1260attccctaaa taacatgatt tatttcctaa tactaagata tagggctcaa ctattggagt 1320tgcaaatgtt ttttggcact aaacacttta tatcaggtcc tattttaatt ttaatttgaa 1380ggactcaaat ataggacttc tcgttagaga tgctcttagc gagtgtttgt gcatgattgc 1440tatttatgtc tgtagtttag ttgggggctt taatatgttt agttgaagtt ctagtatttt 1500ttaggttctc cactctttgg attatgacaa cgaccactat ccaagcagtc tttgagtgca 1560aacgcgcgag caaactatct gatctattaa attatgatcc aaccgttatg tcatattgaa 1620gacttaaacc ctttcaccac cagcccaagt atctttatga aaaaccctaa caaaccacaa 1680ttgcatctat ggttggatta taatttaacg tatcagatgg ttcgcttgca tgcttacata 1740tctagaaact gtttgcataa cagtcgttct ctttggttat ataatgcttt agtaatcatc 1800agccaagtgt aaacaaatgg tacaaactag tagtgaacac atcctcccta cctatctcta 1860ggggtgtaac tagatatccg aattcttaga acaaatttca tattttaaaa tagatatgct 1920tcaaaattta tgctaatctt ttttatatta tcaagcatat tattacacat aagaataaaa 1980ttttgtatag aattttatcc attatttgtt ccctagaatt taaaaagtga aaaaacattc 2040gaatctgtat cagtttcgta ttcaaatttt tacatctatt atttgagaat atatatgata 2100aatttgaggt ttagttttta tgaatcttta caaggttaat gttaaataca tgactatgga 2160tttacatagt aaattctatg tcttatttgt ccgcgattga agaaaaatga caaaaagatc 2220tgacattcga ataaacatct gtttccactc ctacctatct gacctcctat ttcaaactcc 2280actttgtaac acggtacaaa atcactccct acctatctga cctcctattt caaactccac 2340tcagtaaaca atattgtcta tggtacaaaa ccaagtgttt tatacatcta tttgcacgat 2400ctgctcgagt caggcatcct tgacacacaa catactcctt gtggctataa atgtccaaat 2460agagcagacc taatgggtgg accgttgcat gacacgactt atcccaagac gagcacagtt 2520cgccccattg gtcatggggg tccgggctag tctagcctga tcatcgggtc acacttaggc 2580cacaggtgtg ccacaacggg atagcccaac atgtcccttt ttgtcatgca tatatctata 2640ttatagttag tataatgtaa aaaaacaaaa ggtatgtgtg ttatgttggt tagatgtgtt 2700taaataactc tttaaagcta gcaactatgg tttaaatcat acatatacac atttttattt 2760tatttttatt taaacgatat gggccttcta ggcacgtcga gtgtgacggg ccagtgagat 2820gacacattat aattactggt ctagcaggcc gtacctaggt ctttctcgtg ggccaagact 2880aagggttggc ccgttggcta atctgtacgg taccgatact gtcctaattc atttgaacac 2940ctgtagaaga ggggaattta taattgagga ggaatgtact catgcggtac accaggggaa 3000ttgttttgtt gtgctcagcg atagatttca acgcaacggt gagccagttt cactaaaaaa 3060aggggggggg gggggggggg gggaaggcca catcaaaggc gaggtgctga cgagcagaag 3120atgctagcag tgacgccaag tccagcagct agcaatgaaa gggtactcgg gatttaacaa 3180tgcctagaga cggcatcatc ccctcaataa tccggtgctc tctttttgtt tattcaccag 3240ttggcgtagc tatatacaca tgtctggtct gacgaacaaa tcaagggatc gctagctcgg 3300gctagccttc ctatcactgt catgacatgt gctctgcctc tgctggttga taagccgtgc 3360gccttctcgc taattctttc ttgtgctaga ggcgagtcaa acaaacgctg cacctcgtag 3420cccttaatct gcgctaaggg tcacatgacc ctgttcccta tcgctagtta ccaacgaccc 3480attccccctg acagatactt acgacgcgtc cgtacgcggc aggcctcggc agttcggcat 3540caccagcacc ggcgccggca ttcgccccct gccagccggt tcgcagattc gcagggcgga 3600gtcggccgca gttgccgcat cccaaacgcc cgggaacctt tggggcccct ctacgagcaa 3660atgaagttgc tgcccctggc ttcgtaaagc tctgactttt gatcacttga ttggcagtcg 3720tactcctcgc tcataggccg acacggccgc aaagtcaact acccgctccg ccatccttca 3780acccccgcca cgcgcctata tatgttcgcg gccatgtccg tactagtcct ccaacccaca 3840agccacaacc ccgagctcag atccctcgcc tcgtgtcgtg tctccggtcg acgacgacca 3900acagccagtg tgggccagac ggacaccgcc gagctatagc gcttggtgat aaagcttggt 3960cacccggtcc gggcctagaa ggccagcttc aagtttgtac aaaaaagttg aacgagaaac 4020gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata 4080atactgtaaa acacaacata tgcagtcact atgaatcaac tacttagatg gtattagtga 4140cctgtagaat tcgagctcta gagctgcagg gcggccgcga tatcccctat agtgagtcgt 4200attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt 4260acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca 4320gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt 4380ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag 4440gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg 4500gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg 4560aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac 4620tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag 4680gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt 4740gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga 4800ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac 4860aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg 4920gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg 4980ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg 5040gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg 5100atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta 5160attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac 5220caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga 5280tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 5340aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 5400aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt 5460taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 5520taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 5580agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 5640tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca 5700cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 5760agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 5820gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 5880aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 5940tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 6000ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 6060aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 6120ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta 6180ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg 6240cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg 6300cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga 6360caaacaacag ataaaac 63778717777DNAArtificial SeqeunceVector 87attatacaaa gttgatagat atcggaccga ttaaacttta attcggtccg aagcttgcat 60gcctgcagtg cagcgtgacc cggtcgtgcc cctctctaga gataatgagc attgcatgtc 120taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt gcagtttatc 180tatctttata catatattta aactttactc tacgaataat ataatctata gtactacaat 240aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta aaggacaatt 300gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt gttctccttt 360ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta catccattta 420gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt ttattctatt 480ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta ataatttaga 540tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta agaaattaaa 600aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt aaacgccgtc 660gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca 720gacggcacgg catctctgtc gctgcctctg gacccctctc gagagttccg ctccaccgtt 780ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg agcggcagac gtgagccggc 840acggcaggcg gcctcctcct cctctcacgg caccggcagc tacgggggat tcctttccca 900ccgctccttc gctttccctt cctcgcccgc cgtaataaat agacaccccc tccacaccct 960ctttccccaa cctcgtgttg ttcggagcgc acacacacac aaccagatct cccccaaatc 1020cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc tccccccccc ccctctctac 1080cttctctaga tcggcgttcc ggtccatgca tggttagggc ccggtagttc tacttctgtt 1140catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat 1200gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat 1260cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt 1320tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc 1380gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt 1440cgttctagat cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat 1500ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc 1560gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt 1620ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg 1680gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt 1740gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta 1800tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca 1860tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt 1920gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct 1980gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt 2040ttggtgttac ttctgcaggt cgactttaac ttagcctagg atccacacga caccatgtcc 2100cccgagcgcc gccccgtcga gatccgcccg gccaccgccg ccgacatggc cgccgtgtgc 2160gacatcgtga accactacat cgagacctcc accgtgaact tccgcaccga gccgcagacc 2220ccgcaggagt ggatcgacga cctggagcgc ctccaggacc gctacccgtg gctcgtggcc 2280gaggtggagg gcgtggtggc cggcatcgcc tacgccggcc cgtggaaggc ccgcaacgcc 2340tacgactgga ccgtggagtc caccgtgtac gtgtcccacc gccaccagcg cctcggcctc 2400ggctccaccc tctacaccca cctcctcaag agcatggagg cccagggctt caagtccgtg 2460gtggccgtga tcggcctccc gaacgacccg tccgtgcgcc tccacgaggc cctcggctac 2520accgcccgcg gcaccctccg cgccgccggc tacaagcacg gcggctggca cgacgtcggc 2580ttctggcagc gcgacttcga gctgccggcc ccgccgcgcc cggtgcgccc ggtgacgcag 2640atctgagtcg aaacctagac ttgtccatct tctggattgg ccaacttaat taatgtatga 2700aataaaagga tgcacacata gtgacatgct aatcactata atgtgggcat caaagttgtg 2760tgttatgtgt aattactagt tatctgaata aaagagaaag agatcatcca tatttcttat 2820cctaaatgaa tgtcacgtgt ctttataatt ctttgatgaa ccagatgcat ttcattaacc 2880aaatccatat acatataaat attaatcata tataattaat atcaattggg ttagcaaaac 2940aaatctagtc taggtgtgtt ttgcgaattg cggccgccac cgcggtggag

ctcgaattca 3000ttccgattaa tcgtggcctc ttgctcttca ggatgaagag ctatgtttaa acgtgcaagc 3060gctactagac aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg 3120tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc 3180agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg 3240ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg 3300caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta 3360acgatgacag agcgttgctg cctgtgatca aatatcatct ccctcgcaga gatccgaatt 3420atcagccttc ttattcattt ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg 3480ccgacataat aggaaatcgc tggataaagc cgctgaggaa gctgagtggc gctatttctt 3540tagaagtgaa cgttgacgat cgtcgaccgt accccgatga attaattcgg acgtacgttc 3600tgaacacagc tggatactta cttgggcgat tgtcatacat gacatcaaca atgtacccgt 3660ttgtgtaacc gtctcttgga ggttcgtatg acactagtgg ttcccctcag cttgcgacta 3720gatgttgagg cctaacattt tattagagag caggctagtt gcttagatac atgatcttca 3780ggccgttatc tgtcagggca agcgaaaatt ggccatttat gacgaccaat gccccgcaga 3840agctcccatc tttgccgcca tagacgccgc gccccccttt tggggtgtag aacatccttt 3900tgccagatgt ggaaaagaag ttcgttgtcc cattgttggc aatgacgtag tagccggcga 3960aagtgcgaga cccatttgcg ctatatataa gcctacgatt tccgttgcga ctattgtcgt 4020aattggatga actattatcg tagttgctct cagagttgtc gtaatttgat ggactattgt 4080cgtaattgct tatggagttg tcgtagttgc ttggagaaat gtcgtagttg gatggggagt 4140agtcataggg aagacgagct tcatccacta aaacaattgg caggtcagca agtgcctgcc 4200ccgatgccat cgcaagtacg aggcttagaa ccaccttcaa cagatcgcgc atagtcttcc 4260ccagctctct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattg 4320ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtga acaaattctt 4380ccaactgatc tgcgcgcgag gccaagcgat cttcttgtcc aagataagcc tgcctagctt 4440caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat 4500ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta 4560catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta 4620gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta 4680ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg 4740tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt 4800cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta 4860cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca 4920aagctcgccg cgttgtttca tcaagcctta cagtcaccgt aaccagcaaa tcaatatcac 4980tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg 5040gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga 5100tcaccgcttc cctcatgatg tttaactcct gaattaagcc gcgccgcgaa gcggtgtcgg 5160cttgaatgaa ttgttaggcg tcatcctgtg ctcccgagaa ccagtaccag tacatcgctg 5220tttcgttcga gacttgaggt ctagttttat acgtgaacag gtcaatgccg ccgagagtaa 5280agccacattt tgcgtacaaa ttgcaggcag gtacattgtt cgtttgtgtc tctaatcgta 5340tgccaaggag ctgtctgctt agtgcccact ttttcgcaaa ttcgatgaga ctgtgcgcga 5400ctcctttgcc tcggtgcgtg tgcgacacaa caatgtgttc gatagaggct agatcgttcc 5460atgttgagtt gagttcaatc ttcccgacaa gctcttggtc gatgaatgcg ccatagcaag 5520cagagtcttc atcagagtca tcatccgaga tgtaatcctt ccggtagggg ctcacacttc 5580tggtagatag ttcaaagcct tggtcggata ggtgcacatc gaacacttca cgaacaatga 5640aatggttctc agcatccaat gtttccgcca cctgctcagg gatcaccgaa atcttcatat 5700gacgcctaac gcctggcaca gcggatcgca aacctggcgc ggcttttggc acaaaaggcg 5760tgacaggttt gcgaatccgt tgctgccact tgttaaccct tttgccagat ttggtaacta 5820taatttatgt tagaggcgaa gtcttgggta aaaactggcc taaaattgct ggggatttca 5880ggaaagtaaa catcaccttc cggctcgatg tctattgtag atatatgtag tgtatctact 5940tgatcggggg atctgctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 6000gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 6060tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag 6120cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg 6180caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 6240tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 6300tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 6360aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 6420tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 6480tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 6540cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 6600agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 6660tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 6720aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 6780ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 6840cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 6900accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 6960ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 7020ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 7080gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 7140aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 7200gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 7260gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 7320cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 7380gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 7440gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca 7500gggggggggg ggggggggga cttccattgt tcattccacg gacaaaaaca gagaaaggaa 7560acgacagagg ccaaaaagcc tcgctttcag cacctgtcgt ttcctttctt ttcagagggt 7620attttaaata aaaacattaa gttatgacga agaagaacgg aaacgcctta aaccggaaaa 7680ttttcataaa tagcgaaaac ccgcgaggtc gccgccccgt aacctgtcgg atcaccggaa 7740aggacccgta aagtgataat gattatcatc tacatatcac aacgtgcgtg gaggccatca 7800aaccacgtca aataatcaat tatgacgcag gtatcgtatt aattgatctg catcaactta 7860acgtaaaaac aacttcagac aatacaaatc agcgacactg aatacggggc aacctcatgt 7920cccccccccc cccccccctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7980attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 8040agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 8100actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 8160ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8220ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 8280gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8340atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8400cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8460gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8520gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8580ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8640gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattggtcga 8700cgatcttgct gcgttcggat attttcgtgg agttcccgcc acagacccgg attgaaggcg 8760agatccagca actcgcgcca gatcatcctg tgacggaact ttggcgcgtg atgactggcc 8820aggacgtcgg ccgaaagagc gacaagcaga tcacgctttt cgacagcgtc ggatttgcga 8880tcgaggattt ttcggcgctg cgctacgtcc gcgaccgcgt tgagggatca agccacagca 8940gcccactcga ccttctagcc gacccagacg agccaaggga tctttttgga atgctgctcc 9000gtcgtcaggc tttccgacgt ttgggtggtt gaacagaagt cattatcgta cggaatgcca 9060agcactcccg aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac 9120cttttcacgc ccttttaaat atccgttatt ctaataaacg ctcttttctc ttaggtttac 9180ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg 9240atcatgagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc 9300cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag caagctggta 9360cgattgtaat acgactcact atagggcgaa ttgagcgctg tttaaacgct cttcaactgg 9420aagagcggtt accagagctg gtcacctttg tccaccaaga tggaactgcg gccgctcatt 9480aattaagtca ggcgcgcctc tagttgaaga cacgttcatg tcttcatcgt aagaagacac 9540tcagtagtct tcggccagaa tggcccggac cgaagctggc cgctctagaa ctagtggatc 9600tcgatgtgta gtctacgaga agggttaacc gtctcttcgt gagaataacc gtggcctaaa 9660aataagccga tgaggataaa taaaatgtgg tggtacagta cttcaagagg tttactcatc 9720aagaggatgc ttttccgatg agctctagta gtacatcgga cctcacatac ctccattgtg 9780gtgaaatatt ttgtgctcat ttagtgatgg gtaaattttg tttatgtcac tctaggtttt 9840gacatttcag ttttgccact cttaggtttt gacaaataat ttccattccg cggcaaaagc 9900aaaacaattt tattttactt ttaccactct tagctttcac aatgtatcac aaatgccact 9960ctagaaattc tgtttatgcc acagaatgtg aaaaaaaaca ctcacttatt tgaagccaag 10020gtgttcatgg catggaaatg tgacataaag taacgttcgt gtataagaaa aaattgtact 10080cctcgtaaca agagacggaa acatcatgag acaatcgcgt ttggaaggct ttgcatcacc 10140tttggatgat gcgcatgaat ggagtcgtct gcttgctagc cttcgcctac cgcccactga 10200gtccgggcgg caactaccat cggcgaacga cccagctgac ctctaccgac cggacttgaa 10260tgcgctacct tcgtcagcga cgatggccgc gtacgctggc gacgtgcccc cgcatgcatg 10320gcggcacatg gcgagctcag accgtgcgtg gctggctaca aatacgtacc ccgtgagtgc 10380cctagctaga aacttacacc tgcaactgcg agagcgagcg tgtgagtgta gccgagtaga 10440tcccccggtc gccaccatgg cctcctccga gaacgtcatc accgagttca tgcgcttcaa 10500ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 10560ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg gccccctgcc 10620cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca 10680ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg 10740cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc caggactcct ccctgcagga 10800cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt 10860gatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg 10920cgtgctgaag ggcgagaccc acaaggccct gaagctgaag gacggcggcc actacctggt 10980ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt 11040ggacgccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga 11100gcgcaccgag ggccgccacc acctgttcct gtagcggccc atggatattc gaacgcgtag 11160gtaccacatg gttaacctag acttgtccat cttctggatt ggccaactta attaatgtat 11220gaaataaaag gatgcacaca tagtgacatg ctaatcacta taatgtgggc atcaaagttg 11280tgtgttatgt gtaattacta gttatctgaa taaaagagaa agagatcatc catatttctt 11340atcctaaatg aatgtcacgt gtctttataa ttctttgatg aaccagatgc atttcattaa 11400ccaaatccat atacatataa atattaatca tatataatta atatcaattg ggttagcaaa 11460acaaatctag tctaggtgtg ttttgcgaat gcggccgcca ccgcggtgga gctcgaattc 11520cggtccgggc ctagaaggcc atttaaatcc tgaggatctg gtcttcctaa ggacccggga 11580tatcgctatc aactttgtat agaaaagttg ggccgaattc gagctcggta cggccagaat 11640ggcccggacc gggttaccga attcgagctc ggtaccctgg gatccgcaag ggacgaccgt 11700ggtccttgtt tgatttactt ccaggattat ataatccagc ttatggatta tataagtacc 11760tattgacgtc acgtgcttat gtattataat aatctaggta tatagattat ataatctatc 11820taataataat ctgtgttgtt tgtttatctc tcaaaacaaa caggtcctaa aatggtcccg 11880ggcgtccaat gtgtcgtcaa gtagtgttaa gctaaatcga catttctttg tgggttgtgt 11940ggaaggtgtt ccttttcctt aagttgttag ttgtgcaagg tgttccttag agcatctcca 12000ataggaccta taatggattc tattttgaat tataagactc taacaacaaa agcatacttt 12060aatggggatt ctattttaca aaaaaatatc aaatgattat atggtcgatt cctcgggtcc 12120taaatatagt atctcatata atagagctct atcctcattt tatatactat ttttaagttt 12180ttatttacta aataacatga tttattttct aatactatga actcaactat tagagctgta 12240aacgtttttg tggtactaaa cactttaaat caggtcctat tttaatttga aggacttaaa 12300tataagactt ctggttagag atgctcttag cgagtgtttg tgcatgattg ctatttagtc 12360tttgtggatt gtggaaggtg ttacttttcc tcaagttgtt agttgtgcaa ggtgtttctt 12420agagcatctc taacaggagc cttaacggaa tctattttga agtatagtac tttaacacca 12480aaaacatact ttaatagggg tcctatttta caaaaaaatt atcaaatgat tataaggtcc 12540actcctcggg tcctaaatat aatatctcat atactagagc tctatcctca ttttatatac 12600tatccctagg tttttattcc ctaaataaca tgatttattt cctaatacta agatataggg 12660ctcaactatt ggagttgcaa atgttttttg gcactaaaca ctttatatca ggtcctattt 12720taattttaat ttgaaggact caaatatagg acttctcgtt agagatgctc ttagcgagtg 12780tttgtgcatg attgctattt atgtctgtag tttagttggg ggctttaata tgtttagttg 12840aagttctagt attttttagg ttctccactc tttggattat gacaacgacc actatccaag 12900cagtctttga gtgcaaacgc gcgagcaaac tatctgatct attaaattat gatccaaccg 12960ttatgtcata ttgaagactt aaaccctttc accaccagcc caagtatctt tatgaaaaac 13020cctaacaaac cacaattgca tctatggttg gattataatt taacgtatca gatggttcgc 13080ttgcatgctt acatatctag aaactgtttg cataacagtc gttctctttg gttatataat 13140gctttagtaa tcatcagcca agtgtaaaca aatggtacaa actagtagtg aacacatcct 13200ccctacctat ctctaggggt gtaactagat atccgaattc ttagaacaaa tttcatattt 13260taaaatagat atgcttcaaa atttatgcta atctttttta tattatcaag catattatta 13320cacataagaa taaaattttg tatagaattt tatccattat ttgttcccta gaatttaaaa 13380agtgaaaaaa cattcgaatc tgtatcagtt tcgtattcaa atttttacat ctattatttg 13440agaatatata tgataaattt gaggtttagt ttttatgaat ctttacaagg ttaatgttaa 13500atacatgact atggatttac atagtaaatt ctatgtctta tttgtccgcg attgaagaaa 13560aatgacaaaa agatctgaca ttcgaataaa catctgtttc cactcctacc tatctgacct 13620cctatttcaa actccacttt gtaacacggt acaaaatcac tccctaccta tctgacctcc 13680tatttcaaac tccactcagt aaacaatatt gtctatggta caaaaccaag tgttttatac 13740atctatttgc acgatctgct cgagtcaggc atccttgaca cacaacatac tccttgtggc 13800tataaatgtc caaatagagc agacctaatg ggtggaccgt tgcatgacac gacttatccc 13860aagacgagca cagttcgccc cattggtcat gggggtccgg gctagtctag cctgatcatc 13920gggtcacact taggccacag gtgtgccaca acgggatagc ccaacatgtc cctttttgtc 13980atgcatatat ctatattata gttagtataa tgtaaaaaaa caaaaggtat gtgtgttatg 14040ttggttagat gtgtttaaat aactctttaa agctagcaac tatggtttaa atcatacata 14100tacacatttt tattttattt ttatttaaac gatatgggcc ttctaggcac gtcgagtgtg 14160acgggccagt gagatgacac attataatta ctggtctagc aggccgtacc taggtctttc 14220tcgtgggcca agactaaggg ttggcccgtt ggctaatctg tacggtaccg atactgtcct 14280aattcatttg aacacctgta gaagagggga atttataatt gaggaggaat gtactcatgc 14340ggtacaccag gggaattgtt ttgttgtgct cagcgataga tttcaacgca acggtgagcc 14400agtttcacta aaaaaagggg gggggggggg ggggggggaa ggccacatca aaggcgaggt 14460gctgacgagc agaagatgct agcagtgacg ccaagtccag cagctagcaa tgaaagggta 14520ctcgggattt aacaatgcct agagacggca tcatcccctc aataatccgg tgctctcttt 14580ttgtttattc accagttggc gtagctatat acacatgtct ggtctgacga acaaatcaag 14640ggatcgctag ctcgggctag ccttcctatc actgtcatga catgtgctct gcctctgctg 14700gttgataagc cgtgcgcctt ctcgctaatt ctttcttgtg ctagaggcga gtcaaacaaa 14760cgctgcacct cgtagccctt aatctgcgct aagggtcaca tgaccctgtt ccctatcgct 14820agttaccaac gacccattcc ccctgacaga tacttacgac gcgtccgtac gcggcaggcc 14880tcggcagttc ggcatcacca gcaccggcgc cggcattcgc cccctgccag ccggttcgca 14940gattcgcagg gcggagtcgg ccgcagttgc cgcatcccaa acgcccggga acctttgggg 15000cccctctacg agcaaatgaa gttgctgccc ctggcttcgt aaagctctga cttttgatca 15060cttgattggc agtcgtactc ctcgctcata ggccgacacg gccgcaaagt caactacccg 15120ctccgccatc cttcaacccc cgccacgcgc ctatatatgt tcgcggccat gtccgtacta 15180gtcctccaac ccacaagcca caaccccgag ctcagatccc tcgcctcgtg tcgtgtctcc 15240ggtcgacgac gaccaacagc cagtgtgggc cagacggaca ccgccgagct atagcgcttg 15300gtgataaagc ttggtcaccc ggtccgggcc tagaaggcca gcttcaagtt tgtacaaaaa 15360agcaggctcc agcgctcacc atggtccgtc ctgtagaaac cccaacccgt gaaatcaaaa 15420aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt 15480ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc 15540agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct 15600ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt 15660acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat 15720ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtaag tttctgcttc 15780tacctttgat atatatataa taattatcat taattagtag taatataata tttcaaatat 15840ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg 15900tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt gcaggtatca 15960ccgtttgtgt gaacaacgaa ctgaactggc agactatccc gccgggaatg gtgattaccg 16020acgaaaacgg caagaaaaag cagtcttact tccatgattt ctttaactat gccggaatcc 16080atcgcagcgt aatgctctac accacgccga acacctgggt ggacgatatc accgtggtga 16140cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg 16200atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt tgcaactgga caaggcacta 16260gcgggacttt gcaagtggtg aatccgcacc tctggcaacc gggtgaaggt tatctctatg 16320aactgtgcgt cacagccaaa agccagacag agtgtgatat ctacccgctt cgcgtcggca 16380tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa ccacaaaccg ttctacttta 16440ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa aggattcgat aacgtgctga 16500tggtgcacga ccacgcatta atggactgga ttggggccaa ctcctaccgt acctcgcatt 16560acccttacgc tgaagagatg ctcgactggg cagatgaaca tggcatcgtg gtgattgatg 16620aaactgctgc tgtcggcttt aacctctctt taggcattgg tttcgaagcg ggcaacaagc 16680cgaaagaact gtacagcgaa gaggcagtca acggggaaac tcagcaagcg cacttacagg 16740cgattaaaga gctgatagcg cgtgacaaaa accacccaag cgtggtgatg tggagtattg 16800ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag 16860caacgcgtaa actcgacccg acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg 16920ctcacaccga taccatcagc gatctctttg atgtgctgtg cctgaaccgt tattacggat 16980ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt actggaaaaa gaacttctgg 17040cctggcagga gaaactgcat cagccgatta tcatcaccga atacggcgtg gatacgttag 17100ccgggctgca ctcaatgtac accgacatgt ggagtgaaga gtatcagtgt gcatggctgg 17160atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt 17220tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg cggtaacaag aaagggatct 17280tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct gcaaaaacgc tggactggca 17340tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg aagatctccc gggcacccag 17400ctttcttgta caaagtggcc gttaacggat ccagacttgt ccatcttctg gattggccaa 17460cttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc actataatgt 17520gggcatcaaa gttgtgtgtt atgtgtaatt actagttatc tgaataaaag agaaagagat 17580catccatatt tcttatccta aatgaatgtc acgtgtcttt ataattcttt gatgaaccag 17640atgcatttca ttaaccaaat ccatatacat ataaatatta atcatatata attaatatca 17700attgggttag caaaacaaat ctagtctagg tgtgttttgc gaattgcggc aagcttgcgg 17760ccgccccggg caacttt 177778854686DNAArtificial SeqeunceVector 88tctagagctc gttcctcgag gcctcgaggc ctcgaggaac ggtacctgcg gggaagctta 60caataatgtg tgttgttaag tcttgttgcc tgtcatcgtc tgactgactt tcgtcataaa 120tcccggcctc cgtaacccag ctttgggcaa gctcacggat ttgatccggc ggaacgggaa 180tatcgagatg

ccgggctgaa cgctgcagtt ccagctttcc ctttcgggac aggtactcca 240gctgattgat tatctgctga agggtcttgg ttccacctcc tggcacaatg cgaatgatta 300cttgagcgcg atcgggcatc caattttctc ccgtcaggtg cgtggtcaag tgctacaagg 360cacctttcag taacgagcga ccgtcgatcc gtcgccggga tacggacaaa atggagcgca 420gtagtccatc gagggcggcg aaagcctcgc caaaagcaat acgttcatct cgcacagcct 480ccagatccga tcgagggtct tcggcgtagg cagatagaag catggataca ttgcttgaga 540gtattccgat ggactgaagt atggcttcca tcttttctcg tgtgtctgca tctatttcga 600gaaagccccc gatgcggcgc accgcaacgc gaattgccat actatccgaa agtcccagca 660ggcgcgcttg ataggaaaag gtttcatact cggccgatcg cagacgggca ctcacgacct 720tgaacccttc aactttcagg gatcgatgct ggttgatggt agtctcactc gacgtggctc 780tggtgtgttt tgacatagct tcctccaaag aaagcggaag gtctggatac tccagcacga 840aatgtgcccg ggtagacgga tggaagtcta gccctgctca atatgaaatc aacagtacat 900ttacagtcaa tactgaatat acttgctaca tttgcaattg tcttataacg aatgtgaaat 960aaaaatagtg taacaacgct tttactcatc gataatcaca aaaacattta tacgaacaaa 1020aatacaaatg cactccggtt tcacaggata ggcgggatca gaatatgcaa cttttgacgt 1080tttgttcttt caaagggggt gctggcaaaa ccaccgcact catgggcctt tgcgctgctt 1140tggcaaatga cggtaaacga gtggccctct ttgatgccga cgaaaaccgg cctctgacgc 1200gatggagaga aaacgcctta caaagcagta ctgggatcct cgctgtgaag tctattccgc 1260cgacgaaatg ccccttcttg aagcagccta tgaaaatgcc gagctcgaag gatttgatta 1320tgcgttggcc gatacgcgtg gcggctcgag cgagctcaac aacacaatca tcgctagctc 1380aaacctgctt ctgatcccca ccatgctaac gccgctcgac atcgatgagg cactatctac 1440ctaccgctac gtcatcgagc tgctgttgag tgaaaatttg gcaattccta cagctgtttt 1500gcgccaacgc gtcccggtcg gccgattgac aacatcgcaa cgcaggatgt cagagacgct 1560agagagcctt ccagttgtac cgtctcccat gcatgaaaga gatgcatttg ccgcgatgaa 1620agaacgcggc atgttgcatc ttacattact aaacacggga actgatccga cgatgcgcct 1680catagagagg aatcttcgga ttgcgatgga ggaagtcgtg gtcatttcga aactgatcag 1740caaaatcttg gaggcttgaa gatggcaatt cgcaagcccg cattgtcggt cggcgaagca 1800cggcggcttg ctggtgctcg acccgagatc caccatccca acccgacact tgttccccag 1860aagctggacc tccagcactt gcctgaaaaa gccgacgaga aagaccagca acgtgagcct 1920ctcgtcgccg atcacattta cagtcccgat cgacaactta agctaactgt ggatgccctt 1980agtccacctc cgtccccgaa aaagctccag gtttttcttt cagcgcgacc gcccgcgcct 2040caagtgtcga aaacatatga caacctcgtt cggcaataca gtccctcgaa gtcgctacaa 2100atgattttaa ggcgcgcgtt ggacgatttc gaaagcatgc tggcagatgg atcatttcgc 2160gtggccccga aaagttatcc gatcccttca actacagaaa aatccgttct cgttcagacc 2220tcacgcatgt tcccggttgc gttgctcgag gtcgctcgaa gtcattttga tccgttgggg 2280ttggagaccg ctcgagcttt cggccacaag ctggctaccg ccgcgctcgc gtcattcttt 2340gctggagaga agccatcgag caattggtga agagggacct atcggaaccc ctcaccaaat 2400attgagtgta ggtttgaggc cgctggccgc gtcctcagtc accttttgag ccagataatt 2460aagagccaaa tgcaattggc tcaggctgcc atcgtccccc cgtgcgaaac ctgcacgtcc 2520gcgtcaaaga aataaccggc acctcttgct gtttttatca gttgagggct tgacggatcc 2580gcctcaagtt tgcggcgcag ccgcaaaatg agaacatcta tactcctgtc gtaaacctcc 2640tcgtcgcgta ctcgactggc aatgagaagt tgctcgcgcg atagaacgtc gcggggtttc 2700tctaaaaacg cgaggagaag attgaactca cctgccgtaa gtttcacctc accgccagct 2760tcggacatca agcgacgttg cctgagatta agtgtccagt cagtaaaaca aaaagaccgt 2820cggtctttgg agcggacaac gttggggcgc acgcgcaagg caacccgaat gcgtgcaaga 2880aactctctcg tactaaacgg cttagcgata aaatcacttg ctcctagctc gagtgcaaca 2940actttatccg tctcctcaag gcggtcgcca ctgataatta tgattggaat atcagacttt 3000gccgccagat ttcgaacgat ctcaagccca tcttcacgac ctaaatttag atcaacaacc 3060acgacatcga ccgtcgcgga agagagtact ctagtgaact gggtgctgtc ggctaccgcg 3120gtcactttga aggcgtggat cgtaaggtat tcgataataa gatgccgcat agcgacatcg 3180tcatcgataa gaagaacgtg tttcaacggc tcacctttca atctaaaatc tgaacccttg 3240ttcacagcgc ttgagaaatt ttcacgtgaa ggatgtacaa tcatctccag ctaaatgggc 3300agttcgtcag aattgcggct gaccgcggat gacgaaaatg cgaaccaagt atttcaattt 3360tatgacaaaa gttctcaatc gttgttacaa gtgaaacgct tcgaggttac agctactatt 3420gattaaggag atcgcctatg gtctcgcccc ggcgtcgtgc gtccgccgcg agccagatct 3480cgcctacttc ataaacgtcc tcataggcac ggaatggaat gatgacatcg atcgccgtag 3540agagcatgtc aatcagtgtg cgatcttcca agctagcacc ttgggcgcta cttttgacaa 3600gggaaaacag tttcttgaat ccttggattg gattcgcgcc gtgtattgtt gaaatcgatc 3660ccggatgtcc cgagacgact tcactcagat aagcccatgc tgcatcgtcg cgcatctcgc 3720caagcaatat ccggtccggc cgcatacgca gacttgcttg gagcaagtgc tcggcgctca 3780cagcacccag cccagcaccg ttcttggagt agagtagtct aacatgatta tcgtgtggaa 3840tgacgagttc gagcgtatct tctatggtga ttagcctttc ctgggggggg atggcgctga 3900tcaaggtctt gctcattgtt gtcttgccgc ttccggtagg gccacatagc aacatcgtca 3960gtcggctgac gacgcatgcg tgcagaaacg cttccaaatc cccgttgtca aaatgctgaa 4020ggatagcttc atcatcctga ttttggcgtt tccttcgtgt ctgccactgg ttccacctcg 4080aagcatcata acgggaggag acttctttaa gaccagaaac acgcgagctt ggccgtcgaa 4140tggtcaagct gacggtgccc gagggaacgg tcggcggcag acagatttgt agtcgttcac 4200caccaggaag ttcagtggcg cagagggggt tacgtggtcc gacatcctgc tttctcagcg 4260cgcccgctaa aatagcgata tcttcaagat catcataaga gacgggcaaa ggcatcttgg 4320taaaaatgcc ggcttggcgc acaaatgcct ctccaggtcg attgatcgca atttcttcag 4380tcttcgggtc atcgagccat tccaaaatcg gcttcagaag aaagcgtagt tgcggatcca 4440cttccattta caatgtatcc tatctctaag cggaaatttg aattcattaa gagcggcggt 4500tcctcccccg cgtggcgccg ccagtcaggc ggagctggta aacaccaaag aaatcgaggt 4560cccgtgctac gaaaatggaa acggtgtcac cctgattctt cttcagggtt ggcggtatgt 4620tgatggttgc cttaagggct gtctcagttg tctgctcacc gttattttga aagctgttga 4680agctcatccc gccacccgag ctgccggcgt aggtgctagc tgcctggaag gcgccttgaa 4740caacactcaa gagcatagct ccgctaaaac gctgccagaa gtggctgtcg accgagcccg 4800gcaatcctga gcgaccgagt tcgtccgcgc ttggcgatgt taacgagatc atcgcatggt 4860caggtgtctc ggcgcgatcc cacaacacaa aaacgcgccc atctccctgt tgcaagccac 4920gctgtatttc gccaacaacg gtggtgccac gatcaagaag cacgatattg ttcgttgttc 4980cacgaatatc ctgaggcaag acacacttta catagcctgc caaatttgtg tcgattgcgg 5040tttgcaagat gcacggaatt attgtccctt gcgttaccat aaaatcgggg tgcggcaaga 5100gcgtggcgct gctgggctgc agctcggtgg gtttcatacg tatcgacaaa tcgttctcgc 5160cggacacttc gccattcggc aaggagttgt cgtcacgctt gccttcttgt cttcggcccg 5220tgtcgccctg aatggcgcgt ttgctgaccc cttgatcgcc gctgctatat gcaaaaatcg 5280gtgtttcttc cggccgtggc tcatgccgct ccggttcgcc cctcggcggt agaggagcag 5340caggctgaac agcctcttga accgctggag gatccggcgg cacctcaatc ggagctggat 5400gaaatggctt ggtgtttgtt gcgatcaaag ttgacggcga tgcgttctca ttcaccttct 5460tttggcgccc acctagccaa atgaggctta atgataacgc gagaacgaca cctccgacga 5520tcaatttctg agaccccgaa agacgccggc gatgtttgtc ggagaccagg gatccagatg 5580catcaacctc atgtgccgct tgctgactat cgttattcat cccttcgccc ccttcaggac 5640gcgtttcaca tcgggcctca ccgtgcccgt ttgcggcctt tggccaacgg gatcgtaagc 5700ggtgttccag atacatagta ctgtgtggcc atccctcaga cgccaacctc gggaaaccga 5760agaaatctcg acatcgctcc ctttaactga atagttggca acagcttcct tgccatcagg 5820attgatggtg tagatggagg gtatgcgtac attgcccgga aagtggaata ccgtcgtaaa 5880tccattgtcg aagacttcga gtggcaacag cgaacgatcg ccttgggcga cgtagtgcca 5940attactgtcc gccgcaccaa gggctgtgac aggctgatcc aataaattct cagctttccg 6000ttgatattgt gcttccgcgt gtagtctgtc cacaacagcc ttctgttgtg cctcccttcg 6060ccgagccgcc gcatcgtcgg cggggtaggc gaattggacg ctgtaataga gatcgggctg 6120ctctttatcg aggtgggaca gagtcttgga acttatactg aaaacataac ggcgcatccc 6180ggagtcgctt gcggttagca cgattactgg ctgaggcgtg aggacctggc ttgccttgaa 6240aaatagataa tttccccgcg gtagggctgc tagatctttg ctatttgaaa cggcaaccgc 6300tgtcaccgtt tcgttcgtgg cgaatgttac gaccaaagta gctccaaccg ccgtcgagag 6360gcgcaccact tgatcgggat tgtaagccaa ataacgcatg cgcggatcta gcttgcccgc 6420cattggagtg tcttcagcct ccgcaccagt cgcagcggca aataaacatg ctaaaatgaa 6480aagtgctttt ctgatcatgg ttcgctgtgg cctacgtttg aaacggtatc ttccgatgtc 6540tgataggagg tgacaaccag acctgccggg ttggttagtc tcaatctgcc gggcaagctg 6600gtcacctttt cgtagcgaac tgtcgcggtc cacgtactca ccacaggcat tttgccgtca 6660acgacgaggg tccttttata gcgaatttgc tgcgtgcttg gagttacatc atttgaagcg 6720atgtgctcga cctccaccct gccgcgtttg ccaagaatga cttgaggcga actgggattg 6780ggatagttga agaattgctg gtaatcctgg cgcactgttg gggcactgaa gttcgatacc 6840aggtcgtagg cgtactgagc ggtgtcggca tcataactct cgcgcaggcg aacgtactcc 6900cacaatgagg cgttaacgac ggcctcctct tgagttgcag gcaatcgcga gacagacacc 6960tcgctgtcaa cggtgccgtc cggccgtatc catagatata cgggcacaag cctgctcaac 7020ggcaccattg tggctatagc gaacgcttga gcaacatttc ccaaaatcgc gatagctgcg 7080acagctgcaa tgagtttgga gagacgtcgc gccgatttcg ctcgcgcggt ttgaaaggct 7140tctacttcct tatagtgctc ggcaaggctt tcgcgcgcca ctagcatggc atattcaggc 7200cccgtcatag cgtccacccg aattgccgag ctgaagatct gacggagtag gctgccatcg 7260ccccacattc agcgggaaga tcgggccttt gcagctcgct aatgtgtcgt ttgtctggca 7320gccgctcaaa gcgacaacta ggcacagcag gcaatacttc atagaattct ccattgaggc 7380gaatttttgc gcgacctagc ctcgctcaac ctgagcgaag cgacggtaca agctgctggc 7440agattgggtt gcgccgctcc agtaactgcc tccaatgttg ccggcgatcg ccggcaaagc 7500gacaatgagc gcatcccctg tcagaaaaaa catatcgagt tcgtaaagac caatgatctt 7560ggccgcggtc gtaccggcga aggtgattac accaagcata agggtgagcg cagtcgcttc 7620ggttaggatg acgatcgttg ccacgaggtt taagaggaga agcaagagac cgtaggtgat 7680aagttgcccg atccacttag ctgcgatgtc ccgcgtgcga tcaaaaatat atccgacgag 7740gatcagaggc ccgatcgcga gaagcacttt cgtgagaatt ccaacggcgt cgtaaactcc 7800gaaggcagac cagagcgtgc cgtaaaggac ccactgtgcc ccttggaaag caaggatgtc 7860ctggtcgttc atcggaccga tttcggatgc gattttctga aaaacggcct gggtcacggc 7920gaacattgta tccaactgtg ccggaacagt ctgcagaggc aagccggtta cactaaactg 7980ctgaacaaag tttgggaccg tcttttcgaa gatggaaacc acatagtctt ggtagttagc 8040ctgcccaaca attagagcaa caacgatggt gaccgtgatc acccgagtga taccgctacg 8100ggtatcgact tcgccgcgta tgactaaaat accctgaaca ataatccaaa gagtgacaca 8160ggcgatcaat ggcgcactca ccgcctcctg gatagtctca agcatcgagt ccaagcctgt 8220cgtgaaggct acatcgaaga tcgtatgaat ggccgtaaac ggcgccggaa tcgtgaaatt 8280catcgattgg acctgaactt gactggtttg tcgcataatg ttggataaaa tgagctcgca 8340ttcggcgagg atgcgggcgg atgaacaaat cgcccagcct taggggaggg caccaaagat 8400gacagcggtc ttttgatgct ccttgcgttg agcggccgcc tcttccgcct cgtgaaggcc 8460ggcctgcgcg gtagtcatcg ttaataggct tgtcgcctgt acattttgaa tcattgcgtc 8520atggatctgc ttgagaagca aaccattggt cacggttgcc tgcatgatat tgcgagatcg 8580ggaaagctga gcagacgtat cagcattcgc cgtcaagcgt ttgtccatcg tttccagatt 8640gtcagccgca atgccagcgc tgtttgcgga accggtgatc tgcgatcgca acaggtccgc 8700ttcagcatca ctacccacga ctgcacgatc tgtatcgctg gtgatcgcac gtgccgtggt 8760cgacattggc attcgcggcg aaaacatttc attgtctagg tccttcgtcg aaggatactg 8820atttttctgg ttgagcgaag tcagtagtcc agtaacgccg taggccgacg tcaacatcgt 8880aaccatcgct atagtctgag tgagattctc cgcagtcgcg agcgcagtcg cgagcgtctc 8940agcctccgtt gccgggtcgc taacaacaaa ctgcgcccgc gcgggctgaa tatatagaaa 9000gctgcaggtc aaaactgttg caataagttg cgtcgtcttc atcgtttcct accttatcaa 9060tcttctgcct cgtggtgacg ggccatgaat tcgctgagcc agccagatga gttgccttct 9120tgtgcctcgc gtagtcgagt tgcaaagcgc accgtgttgg cacgccccga aagcacggcg 9180acatattcac gcatatcccg cagatcaaat tcgcagatga cgcttccact ttctcgttta 9240agaagaaact tacggctgcc gaccgtcatg tcttcacgga tcgcctgaaa ttccttttcg 9300gtacatttca gtccatcgac ataagccgat cgatctgcgg ttggtgatgg atagaaaatc 9360ttcgtcatac attgcgcaac caagctggct cctagcggcg attccagaac atgctctggt 9420tgctgcgttg ccagtattag catcccgttg ttttttcgaa cggtcaggag gaatttgtcg 9480acgacagtcg aaaatttagg gtttaacaaa taggcgcgaa actcatcgca gctcatcaca 9540aaacggcggc cgtcgatcat ggctccaatc cgatgcagga gatatgctgc agcgggagcg 9600catacttcct cgtattcgag aagatgcgtc atgtcgaagc cggtaatcga cggatctaac 9660tttacttcgt caacttcgcc gtcaaatgcc cagccaagcg catggccccg gcaccagcgt 9720tggagccgcg ctcctgcgcc ttcggcgggc ccatgcaaca aaaattcacg taaccccgcg 9780attgaacgca tttgtggatc aaacgagagc tgacgatgga taccacggac cagacggcgg 9840ttctcttccg gagaaatccc accccgacca tcactctcga tgagagccac gatccattcg 9900cgcagaaaat cgtgtgaggc tgctgtgttt tctaggccac gcaacggcgc caacccgctg 9960ggtgtgcctc tgtgaagtgc caaatatgtt cctcctgtgg cgcgaaccag caattcgcca 10020ccccggtcct tgtcaaagaa cacgaccgta cctgcacggt cgaccatgct ctgttcgagc 10080atggctagaa caaacatcat gagcgtcgtc ttacccctcc cgataggccc gaatattgcc 10140gtcatgccaa catcgtgctc atgcgggata tagtcgaaag gcgttccgcc attggtacga 10200aatcgggcaa tcgcgttgcc ccagtggcct gagctggcgc cctctggaaa gttttcgaaa 10260gagacaaacc ctgcgaaatt gcgtgaagtg attgcgccag ggcgtgtgcg ccacttaaaa 10320ttccccggca attgggacca ataggccgct tccataccaa taccttcttg gacaaccacg 10380gcacctgcat ccgccattcg tgtccgagcc cgcgcgcccc tgtccccaag actattgaga 10440tcgtctgcat agacgcaaag gctcaaatga tgtgagccca taacgaattc gttgctcgca 10500agtgcgtcct cagcctcgga taatttgccg atttgagtca cggctttatc gccggaactc 10560agcatctggc tcgatttgag gctaagtttc gcgtgcgctt gcgggcgagt caggaacgaa 10620aaactctgcg tgagaacaag tggaaaatcg agggatagca gcgcgttgag catgcccggc 10680cgtgtttttg cagggtattc gcgaaacgaa tagatggatc caacgtaact gtcttttggc 10740gttctgatct cgagtcctcg cttgccgcaa atgactctgt cggtataaat cgaagcgccg 10800agtgagccgc tgacgaccgg aaccggtgtg aaccgaccag tcatgatcaa ccgtagcgct 10860tcgccaattt cggtgaagag cacaccctgc ttctcgcgga tgccaagacg atgcaggcca 10920tacgctttaa gagagccagc gacaacatgc caaagatctt ccatgttcct gatctggccc 10980gtgagatcgt tttccctttt tccgcttagc ttggtgaacc tcctctttac cttccctaaa 11040gccgcctgtg ggtagacaat caacgtaagg aagtgttcat tgcggaggag ttggccggag 11100agcacgcgct gttcaaaagc ttcgttcagg ctagcggcga aaacactacg gaagtgtcgc 11160ggcgccgatg atggcacgtc ggcatgacgt acgaggtgag catatattga cacatgatca 11220tcagcgatat tgcgcaacag cgtgttgaac gcacgacaac gcgcattgcg catttcagtt 11280tcctcaagct cgaatgcaac gccatcaatt ctcgcaatgg tcatgatcga tccgtcttca 11340agaaggacga tatggtcgct gaggtggcca atataaggga gatagatctc accggatctt 11400tcggtcgttc cactcgcgcc gagcatcaca ccattcctct ccctcgtggg ggaaccctaa 11460ttggatttgg gctaacagta gcgccccccc aaactgcact atcaatgctt cttcccgcgg 11520tccgcaaaaa tagcaggacg acgctcgccg cattgtagtc tcgctccacg atgagccggg 11580ctgcaaacca taacggcacg agaacgactt cgtagagcgg gttctgaacg ataacgatga 11640caaagccggc gaacatcatg aataaccctg ccaatgtcag tggcacccca agaaacaatg 11700cgggccgtgt ggctgcgagg taaagggtcg attcttccaa acgatcagcc atcaactacc 11760gccagtgagc gtttggccga ggaagctcgc cccaaacatg ataacaatgc cgccgacgac 11820gccggcaacc agcccaagcg aagcccgccc gaacatccag gagatcccga tagcgacaat 11880gccgagaaca gcgagtgact ggccgaacgg accaaggata aacgtgcata tattgttaac 11940cattgtggcg gggtcagtgc cgccacccgc agattgcgct gcggcgggtc cggatgagga 12000aatgctccat gcaattgcac cgcacaagct tggggcgcag ctcgatatca cgcgcatcat 12060cgcattcgag agcgagaggc gatttagatg taaacggtat ctctcaaagc atcgcatcaa 12120tgcgcacctc cttagtataa gtcgaataag acttgattgt cgtctgcgga tttgccgttg 12180tcctggtgtg gcggtggcgg agcgattaaa ccgccagcgc catcctcctg cgagcggcgc 12240tgatatgacc cccaaacatc ccacgtctct tcggatttta gcgcctcgtg atcgtctttt 12300ggaggctcga ttaacgcggg caccagcgat tgagcagctg tttcaacttt tcgcacgtag 12360ccgtttgcaa aaccgccgat gaaattaccg gtgttgtaag cggagatcgc ccgacgaagc 12420gcaaattgct tctcgtcaat cgtttcgccg cctgcataac gacttttcag catgtttgca 12480gcggcagata atgatgtgca cgcctggagc gcaccgtcag gtgtcagacc gagcatagaa 12540aaatttcgag agtttatttg catgaggcca acatccagcg aatgccgtgc atcgagacgg 12600tgcctgacga cttgggttgc ttggctgtga tcttgccagt gaagcgtttc gccggtcgtg 12660ttgtcatgaa tcgctaaagg atcaaagcga ctctccacct tagctatcgc cgcaagcgta 12720gatgtcgcaa ctgatggggc acacttgcga gcaacatggt caaactcagc agatgagagt 12780ggcgtggcaa ggctcgacga acagaaggag accatcaagg caagagaaag cgaccccgat 12840ctcttaagca taccttatct ccttagctcg caactaacac cgcctctccc gttggaagaa 12900gtgcgttgtt ttatgttgaa gattatcggg agggtcggtt actcgaaaat tttcaattgc 12960ttctttatga tttcaattga agcgagaaac ctcgcccggc gtcttggaac gcaacatgga 13020ccgagaaccg cgcatccatg actaagcaac cggatcgacc tattcaggcc gcagttggtc 13080aggtcaggct cagaacgaaa atgctcggcg aggttacgct gtctgtaaac ccattcgatg 13140aacgggaagc ttccttccga ttgctcttgg caggaatatt ggcccatgcc tgcttgcgct 13200ttgcaaatgc tcttatcgcg ttggtatcat atgccttgtc cgccagcaga aacgcactct 13260aagcgattat ttgtaaaaat gtttcggtca tgcggcggtc atgggcttga cccgctgtca 13320gcgcaagacg gatcggtcaa ccgtcggcat cgacaacagc gtgaatcttg gtggtcaaac 13380cgccacggga acgtcccata cagccatcgt cttgatcccg ctgtttcccg tcgccgcatg 13440ttggtggacg cggacacagg aactgtcaat catgacgaca ttctatcgaa agccttggaa 13500atcacactca gaatatgatc ccagacgtct gcctcacgcc atcgtacaaa gcgattgtag 13560caggttgtac aggaaccgta tcgatcagga acgtctgccc agggcgggcc cgtccggaag 13620cgccacaaga tgacattgat cacccgcgtc aacgcgcggc acgcgacgcg gcttatttgg 13680gaacaaagga ctgaacaaca gtccattcga aatcggtgac atcaaagcgg ggacgggtta 13740tcagtggcct ccaagtcaag cctcaatgaa tcaaaatcag accgatttgc aaacctgatt 13800tatgagtgtg cggcctaaat gatgaaatcg tccttctaga tcgcctccgt ggtgtagcaa 13860cacctcgcag tatcgccgtg ctgaccttgg ccagggaatt gactggcaag ggtgctttca 13920catgaccgct cttttggccg cgatagatga tttcgttgct gctttgggca cgtagaagga 13980gagaagtcat atcggagaaa ttcctcctgg cgcgagagcc tgctctatcg cgacggcatc 14040ccactgtcgg gaacagaccg gatcattcac gaggcgaaag tcgtcaacac atgcgttata 14100ggcatcttcc cttgaaggat gatcttgttg ctgccaatct ggaggtgcgg cagccgcagg 14160cagatgcgat ctcagcgcaa cttgcggcaa aacatctcac tcacctgaaa accactagcg 14220agtctcgcga tcagacgaag gccttttact taacgacaca atatccgatg tctgcatcac 14280aggcgtcgct atcccagtca atactaaagc ggtgcaggaa ctaaagatta ctgatgactt 14340aggcgtgcca cgaggcctga gacgacgcgc gtagacagtt ttttgaaatc attatcaaag 14400tgatggcctc cgctgaagcc tatcacctct gcgccggtct gtcggagaga tgggcaagca 14460ttattacggt cttcgcgccc gtacatgcat tggacgattg cagggtcaat ggatctgaga 14520tcatccagag gattgccgcc cttaccttcc gtttcgagtt ggagccagcc cctaaatgag 14580acgacatagt cgacttgatg tgacaatgcc aagagagaga tttgcttaac ccgatttttt 14640tgctcaagcg taagcctatt gaagcttgcc ggcatgacgt ccgcgccgaa agaatatcct 14700acaagtaaaa cattctgcac accgaaatgc ttggtgtaga catcgattat gtgaccaaga 14760tccttagcag tttcgcttgg ggaccgctcc gaccagaaat accgaagtga actgacgcca 14820atgacaggaa tcccttccgt ctgcagatag gtaccatcga tagatctgct gcctcgcgcg 14880tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 14940tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 15000gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 15060tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 15120agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 15180ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 15240ttatccacag

aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 15300gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 15360gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 15420taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 15480accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 15540tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 15600cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 15660agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 15720gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 15780gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 15840tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 15900acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 15960cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 16020acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 16080acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 16140tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 16200ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 16260ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 16320tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 16380aatagtttgc gcaacgttgt tgccattgct gcaggggggg gggggggggg gttccattgt 16440tcattccacg gacaaaaaca gagaaaggaa acgacagagg ccaaaaagct cgctttcagc 16500acctgtcgtt tcctttcttt tcagagggta ttttaaataa aaacattaag ttatgacgaa 16560gaagaacgga aacgccttaa accggaaaat tttcataaat agcgaaaacc cgcgaggtcg 16620ccgccccgta acctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct 16680acatatcaca acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg 16740tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca 16800gcgacactga atacggggca acctcatgtc cccccccccc ccccccctgc aggcatcgtg 16860gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 16920gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 16980gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17040cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17100ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17160accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 17220aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 17280aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 17340caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 17400ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 17460gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 17520cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 17580aggccctttc gtcttcaaga attggtcgac gatcttgctg cgttcggata ttttcgtgga 17640gttcccgcca cagacccgga ttgaaggcga gatccagcaa ctcgcgccag atcatcctgt 17700gacggaactt tggcgcgtga tgactggcca ggacgtcggc cgaaagagcg acaagcagat 17760cacgcttttc gacagcgtcg gatttgcgat cgaggatttt tcggcgctgc gctacgtccg 17820cgaccgcgtt gagggatcaa gccacagcag cccactcgac cttctagccg acccagacga 17880gccaagggat ctttttggaa tgctgctccg tcgtcaggct ttccgacgtt tgggtggttg 17940aacagaagtc attatcgtac ggaatgccaa gcactcccga ggggaaccct gtggttggca 18000tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 18060taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 18120ttaaactgaa ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt 18180atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa 18240cgttgaagga gccactcagc aagctggtac gattgtaata cgactcacta tagggcgaat 18300tgagcgctgt ttaaacgctc ttcaactgga agagcggtta ccagagctgg tcacctttgt 18360ccaccaagat ggaactgcgg ccgctcatta attaagtcag gcgcgcctct agttgaagac 18420acgttcatgt cttcatcgta agaagacact cagtagtctt cggccagaat ggcccggacc 18480gaagctggcc gctctagaac tagtggatct cgatgtgtag tctacgagaa gggttaaccg 18540tctcttcgtg agaataaccg tggcctaaaa ataagccgat gaggataaat aaaatgtggt 18600ggtacagtac ttcaagaggt ttactcatca agaggatgct tttccgatga gctctagtag 18660tacatcggac ctcacatacc tccattgtgg tgaaatattt tgtgctcatt tagtgatggg 18720taaattttgt ttatgtcact ctaggttttg acatttcagt tttgccactc ttaggttttg 18780acaaataatt tccattccgc ggcaaaagca aaacaatttt attttacttt taccactctt 18840agctttcaca atgtatcaca aatgccactc tagaaattct gtttatgcca cagaatgtga 18900aaaaaaacac tcacttattt gaagccaagg tgttcatggc atggaaatgt gacataaagt 18960aacgttcgtg tataagaaaa aattgtactc ctcgtaacaa gagacggaaa catcatgaga 19020caatcgcgtt tggaaggctt tgcatcacct ttggatgatg cgcatgaatg gagtcgtctg 19080cttgctagcc ttcgcctacc gcccactgag tccgggcggc aactaccatc ggcgaacgac 19140ccagctgacc tctaccgacc ggacttgaat gcgctacctt cgtcagcgac gatggccgcg 19200tacgctggcg acgtgccccc gcatgcatgg cggcacatgg cgagctcaga ccgtgcgtgg 19260ctggctacaa atacgtaccc cgtgagtgcc ctagctagaa acttacacct gcaactgcga 19320gagcgagcgt gtgagtgtag ccgagtagat cccccggtcg ccaccatggc ctcctccgag 19380aacgtcatca ccgagttcat gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac 19440gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggccacaa caccgtgaag 19500ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc 19560cagtacggct ccaaggtgta cgtgaagcac cccgccgaca tccccgacta caagaagctg 19620tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg 19680accgtgaccc aggactcctc cctgcaggac ggctgcttca tctacaaggt gaagttcatc 19740ggcgtgaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc 19800tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagaccca caaggccctg 19860aagctgaagg acggcggcca ctacctggtg gagttcaagt ccatctacat ggccaagaag 19920cccgtgcagc tgcccggcta ctactacgtg gacgccaagc tggacatcac ctcccacaac 19980gaggactaca ccatcgtgga gcagtacgag cgcaccgagg gccgccacca cctgttcctg 20040tagcggccca tggatattcg aacgcgtagg taccacatgg ttaacctaga cttgtccatc 20100ttctggattg gccaacttaa ttaatgtatg aaataaaagg atgcacacat agtgacatgc 20160taatcactat aatgtgggca tcaaagttgt gtgttatgtg taattactag ttatctgaat 20220aaaagagaaa gagatcatcc atatttctta tcctaaatga atgtcacgtg tctttataat 20280tctttgatga accagatgca tttcattaac caaatccata tacatataaa tattaatcat 20340atataattaa tatcaattgg gttagcaaaa caaatctagt ctaggtgtgt tttgcgaatg 20400cggccgccac cgcggtggag ctcgaattcc ggtccgggcc tagaaggcca tttaaatcct 20460gaggatctgg tcttcctaag gacccgggat atcgctatca actttgtata gaaaagttgg 20520gccgaattcg agctcggtac ggccagaatg gcccggaccg ggttaccgaa ttcgagctcg 20580gtaccctggg atccgcaagg gacgaccgtg gtccttgttt gatttacttc caggattata 20640taatccagct tatggattat ataagtacct attgacgtca cgtgcttatg tattataata 20700atctaggtat atagattata taatctatct aataataatc tgtgttgttt gtttatctct 20760caaaacaaac aggtcctaaa atggtcccgg gcgtccaatg tgtcgtcaag tagtgttaag 20820ctaaatcgac atttctttgt gggttgtgtg gaaggtgttc cttttcctta agttgttagt 20880tgtgcaaggt gttccttaga gcatctccaa taggacctat aatggattct attttgaatt 20940ataagactct aacaacaaaa gcatacttta atggggattc tattttacaa aaaaatatca 21000aatgattata tggtcgattc ctcgggtcct aaatatagta tctcatataa tagagctcta 21060tcctcatttt atatactatt tttaagtttt tatttactaa ataacatgat ttattttcta 21120atactatgaa ctcaactatt agagctgtaa acgtttttgt ggtactaaac actttaaatc 21180aggtcctatt ttaatttgaa ggacttaaat ataagacttc tggttagaga tgctcttagc 21240gagtgtttgt gcatgattgc tatttagtct ttgtggattg tggaaggtgt tacttttcct 21300caagttgtta gttgtgcaag gtgtttctta gagcatctct aacaggagcc ttaacggaat 21360ctattttgaa gtatagtact ttaacaccaa aaacatactt taataggggt cctattttac 21420aaaaaaatta tcaaatgatt ataaggtcca ctcctcgggt cctaaatata atatctcata 21480tactagagct ctatcctcat tttatatact atccctaggt ttttattccc taaataacat 21540gatttatttc ctaatactaa gatatagggc tcaactattg gagttgcaaa tgttttttgg 21600cactaaacac tttatatcag gtcctatttt aattttaatt tgaaggactc aaatatagga 21660cttctcgtta gagatgctct tagcgagtgt ttgtgcatga ttgctattta tgtctgtagt 21720ttagttgggg gctttaatat gtttagttga agttctagta ttttttaggt tctccactct 21780ttggattatg acaacgacca ctatccaagc agtctttgag tgcaaacgcg cgagcaaact 21840atctgatcta ttaaattatg atccaaccgt tatgtcatat tgaagactta aaccctttca 21900ccaccagccc aagtatcttt atgaaaaacc ctaacaaacc acaattgcat ctatggttgg 21960attataattt aacgtatcag atggttcgct tgcatgctta catatctaga aactgtttgc 22020ataacagtcg ttctctttgg ttatataatg ctttagtaat catcagccaa gtgtaaacaa 22080atggtacaaa ctagtagtga acacatcctc cctacctatc tctaggggtg taactagata 22140tccgaattct tagaacaaat ttcatatttt aaaatagata tgcttcaaaa tttatgctaa 22200tcttttttat attatcaagc atattattac acataagaat aaaattttgt atagaatttt 22260atccattatt tgttccctag aatttaaaaa gtgaaaaaac attcgaatct gtatcagttt 22320cgtattcaaa tttttacatc tattatttga gaatatatat gataaatttg aggtttagtt 22380tttatgaatc tttacaaggt taatgttaaa tacatgacta tggatttaca tagtaaattc 22440tatgtcttat ttgtccgcga ttgaagaaaa atgacaaaaa gatctgacat tcgaataaac 22500atctgtttcc actcctacct atctgacctc ctatttcaaa ctccactttg taacacggta 22560caaaatcact ccctacctat ctgacctcct atttcaaact ccactcagta aacaatattg 22620tctatggtac aaaaccaagt gttttataca tctatttgca cgatctgctc gagtcaggca 22680tccttgacac acaacatact ccttgtggct ataaatgtcc aaatagagca gacctaatgg 22740gtggaccgtt gcatgacacg acttatccca agacgagcac agttcgcccc attggtcatg 22800ggggtccggg ctagtctagc ctgatcatcg ggtcacactt aggccacagg tgtgccacaa 22860cgggatagcc caacatgtcc ctttttgtca tgcatatatc tatattatag ttagtataat 22920gtaaaaaaac aaaaggtatg tgtgttatgt tggttagatg tgtttaaata actctttaaa 22980gctagcaact atggtttaaa tcatacatat acacattttt attttatttt tatttaaacg 23040atatgggcct tctaggcacg tcgagtgtga cgggccagtg agatgacaca ttataattac 23100tggtctagca ggccgtacct aggtctttct cgtgggccaa gactaagggt tggcccgttg 23160gctaatctgt acggtaccga tactgtccta attcatttga acacctgtag aagaggggaa 23220tttataattg aggaggaatg tactcatgcg gtacaccagg ggaattgttt tgttgtgctc 23280agcgatagat ttcaacgcaa cggtgagcca gtttcactaa aaaaaggggg gggggggggg 23340gggggggaag gccacatcaa aggcgaggtg ctgacgagca gaagatgcta gcagtgacgc 23400caagtccagc agctagcaat gaaagggtac tcgggattta acaatgccta gagacggcat 23460catcccctca ataatccggt gctctctttt tgtttattca ccagttggcg tagctatata 23520cacatgtctg gtctgacgaa caaatcaagg gatcgctagc tcgggctagc cttcctatca 23580ctgtcatgac atgtgctctg cctctgctgg ttgataagcc gtgcgccttc tcgctaattc 23640tttcttgtgc tagaggcgag tcaaacaaac gctgcacctc gtagccctta atctgcgcta 23700agggtcacat gaccctgttc cctatcgcta gttaccaacg acccattccc cctgacagat 23760acttacgacg cgtccgtacg cggcaggcct cggcagttcg gcatcaccag caccggcgcc 23820ggcattcgcc ccctgccagc cggttcgcag attcgcaggg cggagtcggc cgcagttgcc 23880gcatcccaaa cgcccgggaa cctttggggc ccctctacga gcaaatgaag ttgctgcccc 23940tggcttcgta aagctctgac ttttgatcac ttgattggca gtcgtactcc tcgctcatag 24000gccgacacgg ccgcaaagtc aactacccgc tccgccatcc ttcaaccccc gccacgcgcc 24060tatatatgtt cgcggccatg tccgtactag tcctccaacc cacaagccac aaccccgagc 24120tcagatccct cgcctcgtgt cgtgtctccg gtcgacgacg accaacagcc agtgtgggcc 24180agacggacac cgccgagcta tagcgcttgg tgataaagct tggtcacccg gtccgggcct 24240agaaggccag cttcaagttt gtacaaaaaa gcaggctcca gcgctcacca tggtccgtcc 24300tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga 24360tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc 24420aattgctgtg ccaggcagtt ttaacgatca gttcgccgat gcagatattc gtaattatgc 24480gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat 24540cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt 24600gatggagcat cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc 24660cgggaaaagt gtacgtaagt ttctgcttct acctttgata tatatataat aattatcatt 24720aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag 24780caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat 24840gaccaaaatt tgttgatgtg caggtatcac cgtttgtgtg aacaacgaac tgaactggca 24900gactatcccg ccgggaatgg tgattaccga cgaaaacggc aagaaaaagc agtcttactt 24960ccatgatttc tttaactatg ccggaatcca tcgcagcgta atgctctaca ccacgccgaa 25020cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg caagactgta accacgcgtc 25080tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca 25140acaggtggtt gcaactggac aaggcactag cgggactttg caagtggtga atccgcacct 25200ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc acagccaaaa gccagacaga 25260gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt 25320cctgattaac cacaaaccgt tctactttac tggctttggt cgtcatgaag atgcggactt 25380gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac cacgcattaa tggactggat 25440tggggccaac tcctaccgta cctcgcatta cccttacgct gaagagatgc tcgactgggc 25500agatgaacat ggcatcgtgg tgattgatga aactgctgct gtcggcttta acctctcttt 25560aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa 25620cggggaaact cagcaagcgc acttacaggc gattaaagag ctgatagcgc gtgacaaaaa 25680ccacccaagc gtggtgatgt ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc 25740acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat 25800cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat accatcagcg atctctttga 25860tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa agcggcgatt tggaaacggc 25920agagaaggta ctggaaaaag aacttctggc ctggcaggag aaactgcatc agccgattat 25980catcaccgaa tacggcgtgg atacgttagc cgggctgcac tcaatgtaca ccgacatgtg 26040gagtgaagag tatcagtgtg catggctgga tatgtatcac cgcgtctttg atcgcgtcag 26100cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt gcgacctcgc aaggcatatt 26160gcgcgttggc ggtaacaaga aagggatctt cactcgcgac cgcaaaccga agtcggcggc 26220ttttctgctg caaaaacgct ggactggcat gaacttcggt gaaaaaccgc agcagggagg 26280caaacaatga agatctcccg ggcacccagc tttcttgtac aaagtggccg ttaacggatc 26340cagacttgtc catcttctgg attggccaac ttaattaatg tatgaaataa aaggatgcac 26400acatagtgac atgctaatca ctataatgtg ggcatcaaag ttgtgtgtta tgtgtaatta 26460ctagttatct gaataaaaga gaaagagatc atccatattt cttatcctaa atgaatgtca 26520cgtgtcttta taattctttg atgaaccaga tgcatttcat taaccaaatc catatacata 26580taaatattaa tcatatataa ttaatatcaa ttgggttagc aaaacaaatc tagtctaggt 26640gtgttttgcg aattgcggca agcttgcggc cgccccgggc aactttatta tacaaagttg 26700atagatatcg gaccgattaa actttaattc ggtccgaagc ttgcatgcct gcagtgcagc 26760gtgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa 26820ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata 26880tatttaaact ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt 26940agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa 27000caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag 27060cttcacctat ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa 27120tggtttttat agactaattt ttttagtaca tctattttat tctattttag cctctaaatt 27180aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata 27240aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac 27300atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg 27360acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc 27420tctgtcgctg cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct 27480gtcggcatcc agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct 27540cctcctcctc tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt 27600tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc 27660gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc 27720tccgcttcaa ggtacgccgc tcgtcctccc ccccccccct ctctaccttc tctagatcgg 27780cgttccggtc catgcatggt tagggcccgg tagttctact tctgttcatg tttgtgttag 27840atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc 27900agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct 27960agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg 28020tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca 28080tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga 28140gtagaattct gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc 28200catacatatt catagttacg aattgaagat gatggatgga aatatcgatc taggataggt 28260atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt 28320gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt 28380ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca 28440tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg 28500ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt 28560gagtacctat ctattataat aaacaagtat gttttataat tattttgatc ttgatatact 28620tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta 28680tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct 28740gcaggtcgac tttaacttag cctaggatcc acacgacacc atgtcccccg agcgccgccc 28800cgtcgagatc cgcccggcca ccgccgccga catggccgcc gtgtgcgaca tcgtgaacca 28860ctacatcgag acctccaccg tgaacttccg caccgagccg cagaccccgc aggagtggat 28920cgacgacctg gagcgcctcc aggaccgcta cccgtggctc gtggccgagg tggagggcgt 28980ggtggccggc atcgcctacg ccggcccgtg gaaggcccgc aacgcctacg actggaccgt 29040ggagtccacc gtgtacgtgt cccaccgcca ccagcgcctc ggcctcggct ccaccctcta 29100cacccacctc ctcaagagca tggaggccca gggcttcaag tccgtggtgg ccgtgatcgg 29160cctcccgaac gacccgtccg tgcgcctcca cgaggccctc ggctacaccg cccgcggcac 29220cctccgcgcc gccggctaca agcacggcgg ctggcacgac gtcggcttct ggcagcgcga 29280cttcgagctg ccggccccgc cgcgcccggt gcgcccggtg acgcagatct gagtcgaaac 29340ctagacttgt ccatcttctg gattggccaa cttaattaat gtatgaaata aaaggatgca 29400cacatagtga catgctaatc actataatgt gggcatcaaa gttgtgtgtt atgtgtaatt 29460actagttatc tgaataaaag agaaagagat catccatatt tcttatccta aatgaatgtc 29520acgtgtcttt ataattcttt gatgaaccag atgcatttca ttaaccaaat ccatatacat 29580ataaatatta atcatatata attaatatca attgggttag caaaacaaat ctagtctagg 29640tgtgttttgc gaattgcggc cgccaccgcg gtggagctcg aattcattcc gattaatcgt 29700ggcctcttgc tcttcaggat gaagagctat gtttaaacgt gcaagcgcta ctagacaatt 29760cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca 29820ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa 29880tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta 29940aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg 30000ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg 30060ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat 30120tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga 30180aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt 30240gacgatcgtc gaccgtaccc cgatgaatta attcggacgt acgttctgaa cacagctgga 30300tacttacttg

ggcgattgtc atacatgaca tcaacaatgt acccgtttgt gtaaccgtct 30360cttggaggtt cgtatgacac tagtggttcc cctcagcttg cgactagatg ttgaggccta 30420acattttatt agagagcagg ctagttgctt agatacatga tcttcaggcc gttatctgtc 30480agggcaagcg aaaattggcc atttatgacg accaatgccc cgcagaagct cccatctttg 30540ccgccataga cgccgcgccc cccttttggg gtgtagaaca tccttttgcc agatgtggaa 30600aagaagttcg ttgtcccatt gttggcaatg acgtagtagc cggcgaaagt gcgagaccca 30660tttgcgctat atataagcct acgatttccg ttgcgactat tgtcgtaatt ggatgaacta 30720ttatcgtagt tgctctcaga gttgtcgtaa tttgatggac tattgtcgta attgcttatg 30780gagttgtcgt agttgcttgg agaaatgtcg tagttggatg gggagtagtc atagggaaga 30840cgagcttcat ccactaaaac aattggcagg tcagcaagtg cctgccccga tgccatcgca 30900agtacgaggc ttagaaccac cttcaacaga tcgcgcatag tcttccccag ctctctaacg 30960cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg 31020ccgactacct tggtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg 31080cgcgaggcca agcgatcttc ttgtccaaga taagcctgcc tagcttcaag tatgacgggc 31140tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt 31200ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg 31260ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga 31320tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta 31380tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag 31440atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga 31500taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc 31560tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt 31620gtttcatcaa gccttacagt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg 31680ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc 31740tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc 31800atgatgttta actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt 31860taggcgtcat cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact 31920tgaggtctag ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg 31980tacaaattgc aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt 32040ctgcttagtg cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg 32100tgcgtgtgcg acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt 32160tcaatcttcc cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca 32220gagtcatcat ccgagatgta atccttccgg taggggctca cacttctggt agatagttca 32280aagccttggt cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca 32340tccaatgttt ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct 32400ggcacagcgg atcgcaaacc tggcgcggct tttggcacaa aaggcgtgac aggtttgcga 32460atccgttgct gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga 32520ggcgaagtct tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc 32580accttccggc tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgggggatct 32640gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 32700cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 32760cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 32820atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 32880tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 32940gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 33000ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 33060aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 33120ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 33180aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 33240gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 33300tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 33360tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 33420gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 33480cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 33540cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 33600agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 33660caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 33720ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 33780aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 33840tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 33900agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 33960gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 34020accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 34080tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 34140tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcagggg gggggggggg 34200gggggacttc cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa 34260aaagcctcgc tttcagcacc tgtcgtttcc tttcttttca gagggtattt taaataaaaa 34320cattaagtta tgacgaagaa gaacggaaac gccttaaacc ggaaaatttt cataaatagc 34380gaaaacccgc gaggtcgccg ccccgtaacc tgtcggatca ccggaaagga cccgtaaagt 34440gataatgatt atcatctaca tatcacaacg tgcgtggagg ccatcaaacc acgtcaaata 34500atcaattatg acgcaggtat cgtattaatt gatctgcatc aacttaacgt aaaaacaact 34560tcagacaata caaatcagcg acactgaata cggggcaacc tcatgtcccc cccccccccc 34620cccctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 34680cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 34740tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 34800cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 34860agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 34920cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 34980aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 35040aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 35100gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 35160gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 35220tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 35280ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 35340aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cggagctttt gccattctca 35400ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg 35460aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt 35520gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa 35580aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag 35640tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga 35700cgggacggcg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc 35760atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt 35820ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg 35880cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgccaga 35940aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa 36000agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct 36060acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc 36120ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat 36180caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc 36240ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc 36300gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag 36360cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc 36420ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg 36480gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc 36540gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc 36600gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa 36660ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag 36720ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat 36780aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag 36840cggaggctgg tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc 36900actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg 36960cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat 37020gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg 37080cggccaatct tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca 37140gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact 37200ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac 37260ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg 37320ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc 37380aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta 37440cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc 37500gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt 37560tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt 37620gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc 37680cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc 37740ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg 37800ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt 37860caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc 37920ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt 37980gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc 38040gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag 38100gcgacttccg gcaagctata cgcgccctag gagtgcggtt ggaacgttgg cccagccaga 38160tactcccgat cacgagcagg acgccgatga tttgaagcgc actcagcgtc tgatccaaga 38220acaaccatcc tagcaacacg gcggtccccg ggctgagaaa gcccagtaag gaaacaactg 38280taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca 38340ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa 38400acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg 38460tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa 38520ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca 38580ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg 38640ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg 38700tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg 38760aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc 38820ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc 38880ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct 38940tggggccgtc ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc 39000cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt 39060gctcgtaaac ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc 39120ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca 39180attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg 39240agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc 39300tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc 39360atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc 39420gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt 39480ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc 39540cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa 39600cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc 39660caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt 39720gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg 39780gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc 39840gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc 39900ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac 39960cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg 40020ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga 40080aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc 40140gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc 40200tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga 40260acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg 40320ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg 40380cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc 40440gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg 40500gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc 40560cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc 40620gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc 40680caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt 40740gcgctcctgc ttgtcgccgg catcgttgcg ccactcttca ttaaccgcta tatcgaaaat 40800tgcttgcggc ttgttagaat tgccatgacg tacctcggtg tcacgggtaa gattaccgat 40860aaactggaac tgattatggc tcatatcgaa agtctccttg agaaaggaga ctctagttta 40920gctaaacatt ggttccgctg tcaagaactt tagcggctaa aattttgcgg gccgcgacca 40980aaggtgcgag gggcggcttc cgctgtgtac aaccagatat ttttcaccaa catccttcgt 41040ctgctcgatg agcggggcat gacgaaacat gagctgtcgg agagggcagg ggtttcaatt 41100tcgtttttat cagacttaac caacggtaag gccaacccct cgttgaaggt gatggaggcc 41160attgccgacg ccctggaaac tcccctacct cttctcctgg agtccaccga ccttgaccgc 41220gaggcactcg cggagattgc gggtcatcct ttcaagagca gcgtgccgcc cggatacgaa 41280cgcatcagtg tggttttgcc gtcacataag gcgtttatcg taaagaaatg gggcgacgac 41340acccgaaaaa agctgcgtgg aaggctctga cgccaagggt tagggcttgc acttccttct 41400ttagccgcta aaacggcccc ttctctgcgg gccgtcggct cgcgcatcat atcgacatcc 41460tcaacggaag ccgtgccgcg aatggcatcg ggcgggtgcg ctttgacagt tgttttctat 41520cagaacccct acgtcgtgcg gttcgattag ctgtttgtct tgcaggctaa acactttcgg 41580tatatcgttt gcctgtgcga taatgttgct aatgatttgt tgcgtagggg ttactgaaaa 41640gtgagcggga aagaagagtt tcagaccatc aaggagcggg ccaagcgcaa gctggaacgc 41700gacatgggtg cggacctgtt ggccgcgctc aacgacccga aaaccgttga agtcatgctc 41760aacgcggacg gcaaggtgtg gcacgaacgc cttggcgagc cgatgcggta catctgcgac 41820atgcggccca gccagtcgca ggcgattata gaaacggtgg ccggattcca cggcaaagag 41880gtcacgcggc attcgcccat cctggaaggc gagttcccct tggatggcag ccgctttgcc 41940ggccaattgc cgccggtcgt ggccgcgcca acctttgcga tccgcaagcg cgcggtcgcc 42000atcttcacgc tggaacagta cgtcgaggcg ggcatcatga cccgcgagca atacgaggtc 42060attaaaagcg ccgtcgcggc gcatcgaaac atcctcgtca ttggcggtac tggctcgggc 42120aagaccacgc tcgtcaacgc gatcatcaat gaaatggtcg ccttcaaccc gtctgagcgc 42180gtcgtcatca tcgaggacac cggcgaaatc cagtgcgccg cagagaacgc cgtccaatac 42240cacaccagca tcgacgtctc gatgacgctg ctgctcaaga caacgctgcg tatgcgcccc 42300gaccgcatcc tggtcggtga ggtacgtggc cccgaagccc ttgatctgtt gatggcctgg 42360aacaccgggc atgaaggagg tgccgccacc ctgcacgcaa acaaccccaa agcgggcctg 42420agccggctcg ccatgcttat cagcatgcac ccggattcac cgaaacccat tgagccgctg 42480attggcgagg cggttcatgt ggtcgtccat atcgccagga cccctagcgg ccgtcgagtg 42540caagaaattc tcgaagttct tggttacgag aacggccagt acatcaccaa aaccctgtaa 42600ggagtatttc caatgacaac ggctgttccg ttccgtctga ccatgaatcg cggcattttg 42660ttctaccttg ccgtgttctt cgttctcgct ctcgcgttat ccgcgcatcc ggcgatggcc 42720tcggaaggca ccggcggcag cttgccatat gagagctggc tgacgaacct gcgcaactcc 42780gtaaccggcc cggtggcctt cgcgctgtcc atcatcggca tcgtcgtcgc cggcggcgtg 42840ctgatcttcg gcggcgaact caacgccttc ttccgaaccc tgatcttcct ggttctggtg 42900atggcgctgc tggtcggcgc gcagaacgtg atgagcacct tcttcggtcg tggtgccgaa 42960atcgcggccc tcggcaacgg ggcgctgcac caggtgcaag tcgcggcggc ggatgccgtg 43020cgtgcggtag cggctggacg gctcgcctaa tcatggctct gcgcacgatc cccatccgtc 43080gcgcaggcaa ccgagaaaac ctgttcatgg gtggtgatcg tgaactggtg atgttctcgg 43140gcctgatggc gtttgcgctg attttcagcg cccaagagct gcgggccacc gtggtcggtc 43200tgatcctgtg gttcggggcg ctctatgcgt tccgaatcat ggcgaaggcc gatccgaaga 43260tgcggttcgt gtacctgcgt caccgccggt acaagccgta ttacccggcc cgctcgaccc 43320cgttccgcga gaacaccaat agccaaggga agcaataccg atgatccaag caattgcgat 43380tgcaatcgcg ggcctcggcg cgcttctgtt gttcatcctc tttgcccgca tccgcgcggt 43440cgatgccgaa ctgaaactga aaaagcatcg ttccaaggac gccggcctgg ccgatctgct 43500caactacgcc gctgtcgtcg atgacggcgt aatcgtgggc aagaacggca gctttatggc 43560tgcctggctg tacaagggcg atgacaacgc aagcagcacc gaccagcagc gcgaagtagt 43620gtccgcccgc atcaaccagg ccctcgcggg cctgggaagt gggtggatga tccatgtgga 43680cgccgtgcgg cgtcctgctc cgaactacgc ggagcggggc ctgtcggcgt tccctgaccg 43740tctgacggca gcgattgaag aagagcgctc ggtcttgcct tgctcgtcgg tgatgtactt 43800caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac 43860gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca 43920cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga 43980ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca 44040ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt 44100ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca 44160tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct 44220tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat 44280ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca 44340ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta 44400tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa 44460atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga 44520agcagggtta tgcagcggaa aagcgctgct tccctgctgt tttgtggaat atctaccgac 44580tggaaacagg caaatgcagg aaattactga actgagggga caggcgagag acgatgccaa 44640agagctacac cgacgagctg gccgagtggg ttgaatcccg cgcggccaag aagcgccggc 44700gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga tgtcgaggcg gcgttagcgt 44760ccggctatgc gctcgtcacc atttgggagc acatgcggga aacggggaag gtcaagttct 44820cctacgagac gttccgctcg cacgccaggc ggcacatcaa ggccaagccc gccgatgtgc 44880ccgcaccgca ggccaaggct gcggaacccg cgccggcacc caagacgccg gagccacggc 44940ggccgaagca ggggggcaag gctgaaaagc cggcccccgc tgcggccccg accggcttca 45000ccttcaaccc aacaccggac aaaaaggatc tactgtaatg gcgaaaattc acatggtttt 45060gcagggcaag ggcggggtcg gcaagtcggc catcgccgcg atcattgcgc agtacaagat 45120ggacaagggg cagacaccct tgtgcatcga caccgacccg gtgaacgcga cgttcgaggg 45180ctacaaggcc ctgaacgtcc gccggctgaa catcatggcc ggcgacgaaa ttaactcgcg 45240caacttcgac accctggtcg agctgattgc gccgaccaag gatgacgtgg tgatcgacaa 45300cggtgccagc tcgttcgtgc ctctgtcgca ttacctcatc agcaaccagg tgccggctct 45360gctgcaagaa

atggggcatg agctggtcat ccataccgtc gtcaccggcg gccaggctct 45420cctggacacg gtgagcggct tcgcccagct cgccagccag ttcccggccg aagcgctttt 45480cgtggtctgg ctgaacccgt attgggggcc tatcgagcat gagggcaaga gctttgagca 45540gatgaaggcg tacacggcca acaaggcccg cgtgtcgtcc atcatccaga ttccggccct 45600caaggaagaa acctacggcc gcgatttcag cgacatgctg caagagcggc tgacgttcga 45660ccaggcgctg gccgatgaat cgctcacgat catgacgcgg caacgcctca agatcgtgcg 45720gcgcggcctg tttgaacagc tcgacgcggc ggccgtgcta tgagcgacca gattgaagag 45780ctgatccggg agattgcggc caagcacggc atcgccgtcg gccgcgacga cccggtgctg 45840atcctgcata ccatcaacgc ccggctcatg gccgacagtg cggccaagca agaggaaatc 45900cttgccgcgt tcaaggaaga gctggaaggg atcgcccatc gttggggcga ggacgccaag 45960gccaaagcgg agcggatgct gaacgcggcc ctggcggcca gcaaggacgc aatggcgaag 46020gtaatgaagg acagcgccgc gcaggcggcc gaagcgatcc gcagggaaat cgacgacggc 46080cttggccgcc agctcgcggc caaggtcgcg gacgcgcggc gcgtggcgat gatgaacatg 46140atcgccggcg gcatggtgtt gttcgcggcc gccctggtgg tgtgggcctc gttatgaatc 46200gcagaggcgc agatgaaaaa gcccggcgtt gccgggcttt gtttttgcgt tagctgggct 46260tgtttgacag gcccaagctc tgactgcgcc cgcgctcgcg ctcctgggcc tgtttcttct 46320cctgctcctg cttgcgcatc agggcctggt gccgtcgggc tgcttcacgc atcgaatccc 46380agtcgccggc cagctcggga tgctccgcgc gcatcttgcg cgtcgccagt tcctcgatct 46440tgggcgcgtg aatgcccatg ccttccttga tttcgcgcac catgtccagc cgcgtgtgca 46500gggtctgcaa gcgggcttgc tgttgggcct gctgctgctg ccaggcggcc tttgtacgcg 46560gcagggacag caagccgggg gcattggact gtagctgctg caaacgcgcc tgctgacggt 46620ctacgagctg ttctaggcgg tcctcgatgc gctccacctg gtcatgcttt gcctgcacgt 46680agagcgcaag ggtctgctgg taggtctgct cgatgggcgc ggattctaag agggcctgct 46740gttccgtctc ggcctcctgg gccgcctgta gcaaatcctc gccgctgttg ccgctggact 46800gctttactgc cggggactgc tgttgccctg ctcgcgccgt cgtcgcagtt cggcttgccc 46860ccactcgatt gactgcttca tttcgagccg cagcgatgcg atctcggatt gcgtcaacgg 46920acggggcagc gcggaggtgt ccggcttctc cttgggtgag tcggtcgatg ccatagccaa 46980aggtttcctt ccaaaatgcg tccattgctg gaccgtgttt ctcattgatg cccgcaagca 47040tcttcggctt gaccgccagg tcaagcgcgc cttcatgggc ggtcatgacg gacgccgcca 47100tgaccttgcc gccgttgttc tcgatgtagc cgcgtaatga ggcaatggtg ccgcccatcg 47160tcagcgtgtc atcgacaacg atgtacttct ggccggggat cacctccccc tcgaaagtcg 47220ggttgaacgc caggcgatga tctgaaccgg ctccggttcg ggcgaccttc tcccgctgca 47280caatgtccgt ttcgacctca aggccaaggc ggtcggccag aacgaccgcc atcatggccg 47340gaatcttgtt gttccccgcc gcctcgacgg cgaggactgg aacgatgcgg ggcttgtcgt 47400cgccgatcag cgtcttgagc tgggcaacag tgtcgtccga aatcaggcgc tcgaccaaat 47460taagcgccgc ttccgcgtcg ccctgcttcg cagcctggta ttcaggctcg ttggtcaaag 47520aaccaaggtc gccgttgcga accaccttcg ggaagtctcc ccacggtgcg cgctcggctc 47580tgctgtagct gctcaagacg cctccctttt tagccgctaa aactctaacg agtgcgcccg 47640cgactcaact tgacgctttc ggcacttacc tgtgccttgc cacttgcgtc ataggtgatg 47700cttttcgcac tcccgatttc aggtacttta tcgaaatctg accgggcgtg cattacaaag 47760ttcttcccca cctgttggta aatgctgccg ctatctgcgt ggacgatgct gccgtcgtgg 47820cgctgcgact tatcggcctt ttgggccata tagatgttgt aaatgccagg tttcagggcc 47880ccggctttat ctaccttctg gttcgtccat gcgccttggt tctcggtctg gacaattctt 47940tgcccattca tgaccaggag gcggtgtttc attgggtgac tcctgacggt tgcctctggt 48000gttaaacgtg tcctggtcgc ttgccggcta aaaaaaagcc gacctcggca gttcgaggcc 48060ggctttccct agagccgggc gcgtcaaggt tgttccatct attttagtga actgcgttcg 48120atttatcagt tactttcctc ccgctttgtg tttcctccca ctcgtttccg cgtctagccg 48180acccctcaac atagcggcct cttcttgggc tgcctttgcc tcttgccgcg cttcgtcacg 48240ctcggcttgc accgtcgtaa agcgctcggc ctgcctggcc gcctcttgcg ccgccaactt 48300cctttgctcc tggtgggcct cggcgtcggc ctgcgccttc gctttcaccg ctgccaactc 48360cgtgcgcaaa ctctccgctt cgcgcctggt ggcgtcgcgc tcgccgcgaa gcgcctgcat 48420ttcctggttg gccgcgtcca gggtcttgcg gctctcttct ttgaatgcgc gggcgtcctg 48480gtgagcgtag tccagctcgg cgcgcagctc ctgcgctcga cgctccacct cgtcggcccg 48540ctgcgtcgcc agcgcggccc gctgctcggc tcctgccagg gcggtgcgtg cttcggccag 48600ggcttgccgc tggcgtgcgg ccagctcggc cgcctcggcg gcctgctgct ctagcaatgt 48660aacgcgcgcc tgggcttctt ccagctcgcg ggcctgcgcc tcgaaggcgt cggccagctc 48720cccgcgcacg gcttccaact cgttgcgctc acgatcccag ccggcttgcg ctgcctgcaa 48780cgattcattg gcaagggcct gggcggcttg ccagagggcg gccacggcct ggttgccggc 48840ctgctgcacc gcgtccggca cctggactgc cagcggggcg gcctgcgccg tgcgctggcg 48900tcgccattcg cgcatgccgg cgctggcgtc gttcatgttg acgcgggcgg ccttacgcac 48960tgcatccacg gtcgggaagt tctcccggtc gccttgctcg aacagctcgt ccgcagccgc 49020aaaaatgcgg tcgcgcgtct ctttgttcag ttccatgttg gctccggtaa ttggtaagaa 49080taataatact cttacctacc ttatcagcgc aagagtttag ctgaacagtt ctcgacttaa 49140cggcaggttt tttagcggct gaagggcagg caaaaaaagc cccgcacggt cggcgggggc 49200aaagggtcag cgggaagggg attagcgggc gtcgggcttc ttcatgcgtc ggggccgcgc 49260ttcttgggat ggagcacgac gaagcgcgca cgcgcatcgt cctcggccct atcggcccgc 49320gtcgcggtca ggaacttgtc gcgcgctagg tcctccctgg tgggcaccag gggcatgaac 49380tcggcctgct cgatgtaggt ccactccatg accgcatcgc agtcgaggcc gcgttccttc 49440accgtctctt gcaggtcgcg gtacgcccgc tcgttgagcg gctggtaacg ggccaattgg 49500tcgtaaatgg ctgtcggcca tgagcggcct ttcctgttga gccagcagcc gacgacgaag 49560ccggcaatgc aggcccctgg cacaaccagg ccgacgccgg gggcagggga tggcagcagc 49620tcgccaacca ggaaccccgc cgcgatgatg ccgatgccgg tcaaccagcc cttgaaacta 49680tccggccccg aaacacccct gcgcattgcc tggatgctgc gccggatagc ttgcaacatc 49740aggagccgtt tcttttgttc gtcagtcatg gtccgccctc accagttgtt cgtatcggtg 49800tcggacgaac tgaaatcgca agagctgccg gtatcggtcc agccgctgtc cgtgtcgctg 49860ctgccgaagc acggcgaggg gtccgcgaac gccgcagacg gcgtatccgg ccgcagcgca 49920tcgcccagca tggccccggt cagcgagccg ccggccaggt agcccagcat ggtgctgttg 49980gtcgccccgg ccaccagggc cgacgtgacg aaatcgccgt cattccctct ggattgttcg 50040ctgctcggcg gggcagtgcg ccgcgccggc ggcgtcgtgg atggctcggg ttggctggcc 50100tgcgacggcc ggcgaaaggt gcgcagcagc tcgttatcga ccggctgcgg cgtcggggcc 50160gccgccttgc gctgcggtcg gtgttccttc ttcggctcgc gcagcttgaa cagcatgatc 50220gcggaaacca gcagcaacgc cgcgcctacg cctcccgcga tgtagaacag catcggattc 50280attcttcggt cctccttgta gcggaaccgt tgtctgtgcg gcgcgggtgg cccgcgccgc 50340tgtctttggg gatcagccct cgatgagcgc gaccagtttc acgtcggcaa ggttcgcctc 50400gaactcctgg ccgtcgtcct cgtacttcaa ccaggcatag ccttccgccg gcggccgacg 50460gttgaggata aggcgggcag ggcgctcgtc gtgctcgacc tggacgatgg cctttttcag 50520cttgtccggg tccggctcct tcgcgccctt ttccttggcg tccttaccgt cctggtcgcc 50580gtcctcgccg tcctggccgt cgccggcctc cgcgtcacgc tcggcatcag tctggccgtt 50640gaaggcatcg acggtgttgg gatcgcggcc cttctcgtcc aggaactcgc gcagcagctt 50700gaccgtgccg cgcgtgattt cctgggtgtc gtcgtcaagc cacgcctcga cttcctccgg 50760gcgcttcttg aaggccgtca ccagctcgtt caccacggtc acgtcgcgca cgcggccggt 50820gttgaacgca tcggcgatct tctccggcag gtccagcagc gtgacgtgct gggtgatgaa 50880cgccggcgac ttgccgattt ccttggcgat atcgcctttc ttcttgccct tcgccagctc 50940gcggccaatg aagtcggcaa tttcgcgcgg ggtcagctcg ttgcgttgca ggttctcgat 51000aacctggtcg gcttcgttgt agtcgttgtc gatgaacgcc gggatggact tcttgccggc 51060ccacttcgag ccacggtagc ggcgggcgcc gtgattgatg atatagcggc ccggctgctc 51120ctggttctcg cgcaccgaaa tgggtgactt caccccgcgc tctttgatcg tggcaccgat 51180ttccgcgatg ctctccgggg aaaagccggg gttgtcggcc gtccgcggct gatgcggatc 51240ttcgtcgatc aggtccaggt ccagctcgat agggccggaa ccgccctgag acgccgcagg 51300agcgtccagg aggctcgaca ggtcgccgat gctatccaac cccaggccgg acggctgcgc 51360cgcgcctgcg gcttcctgag cggccgcagc ggtgtttttc ttggtggtct tggcttgagc 51420cgcagtcatt gggaaatctc catcttcgtg aacacgtaat cagccagggc gcgaacctct 51480ttcgatgcct tgcgcgcggc cgttttcttg atcttccaga ccggcacacc ggatgcgagg 51540gcatcggcga tgctgctgcg caggccaacg gtggccggaa tcatcatctt ggggtacgcg 51600gccagcagct cggcttggtg gcgcgcgtgg cgcggattcc gcgcatcgac cttgctgggc 51660accatgccaa ggaattgcag cttggcgttc ttctggcgca cgttcgcaat ggtcgtgacc 51720atcttcttga tgccctggat gctgtacgcc tcaagctcga tgggggacag cacatagtcg 51780gccgcgaaga gggcggccgc caggccgacg ccaagggtcg gggccgtgtc gatcaggcac 51840acgtcgaagc cttggttcgc cagggccttg atgttcgccc cgaacagctc gcgggcgtcg 51900tccagcgaca gccgttcggc gttcgccagt accgggttgg actcgatgag ggcgaggcgc 51960gcggcctggc cgtcgccggc tgcgggtgcg gtttcggtcc agccgccggc agggacagcg 52020ccgaacagct tgcttgcatg caggccggta gcaaagtcct tgagcgtgta ggacgcattg 52080ccctgggggt ccaggtcgat cacggcaacc cgcaagccgc gctcgaaaaa gtcgaaggca 52140agatgcacaa gggtcgaagt cttgccgacg ccgcctttct ggttggccgt gaccaaagtt 52200ttcatcgttt ggtttcctgt tttttcttgg cgtccgcttc ccacttccgg acgatgtacg 52260cctgatgttc cggcagaacc gccgttaccc gcgcgtaccc ctcgggcaag ttcttgtcct 52320cgaacgcggc ccacacgcga tgcaccgctt gcgacactgc gcccctggtc agtcccagcg 52380acgttgcgaa cgtcgcctgt ggcttcccat cgactaagac gccccgcgct atctcgatgg 52440tctgctgccc cacttccagc ccctggatcg cctcctggaa ctggctttcg gtaagccgtt 52500tcttcatgga taacacccat aatttgctcc gcgccttggt tgaacatagc ggtgacagcc 52560gccagcacat gagagaagtt tagctaaaca tttctcgcac gtcaacacct ttagccgcta 52620aaactcgtcc ttggcgtaac aaaacaaaag cccggaaacc gggctttcgt ctcttgccgc 52680ttatggctct gcacccggct ccatcaccaa caggtcgcgc acgcgcttca ctcggttgcg 52740gatcgacact gccagcccaa caaagccggt tgccgccgcc gccaggatcg cgccgatgat 52800gccggccaca ccggccatcg cccaccaggt cgccgccttc cggttccatt cctgctggta 52860ctgcttcgca atgctggacc tcggctcacc ataggctgac cgctcgatgg cgtatgccgc 52920ttctcccctt ggcgtaaaac ccagcgccgc aggcggcatt gccatgctgc ccgccgcttt 52980cccgaccacg acgcgcgcac caggcttgcg gtccagacct tcggccacgg cgagctgcgc 53040aaggacataa tcagccgccg acttggctcc acgcgcctcg atcagctctt gcactcgcgc 53100gaaatccttg gcctccacgg ccgccatgaa tcgcgcacgc ggcgaaggct ccgcagggcc 53160ggcgtcgtga tcgccgccga gaatgccctt caccaagttc gacgacacga aaatcatgct 53220gacggctatc accatcatgc agacggatcg cacgaacccg ctgaattgaa cacgagcacg 53280gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag 53340tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca 53400ctgcccggca cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg 53460ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg 53520actgccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg 53580ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt 53640cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat 53700tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt 53760gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca 53820tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc 53880gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg 53940gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc 54000cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca 54060agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca 54120acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg 54180gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc 54240gctggaagcc ccgtagcgac gcggagaggg gcgagacaag ccaagggcgc aggctcgatg 54300cgcagcacga catagccggt tctcgcaagg acgagaattt ccctgcggtg cccctcaagt 54360gtcaatgaaa gtttccaacg cgagccattc gcgagagcct tgagtccacg ctagatgaga 54420gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct 54480gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa 54540caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca 54600tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat 54660attcaacggg aaacgtcttg ctcgac 54686893324DNAZea mays 89tggtccttgt ttgatttact tccaggatta tataatccag cttatggatt atataagtac 60ctattgacgt cacgtgctta tgtattataa taatctaggt atatagatta tataatctat 120ctaataataa tctgtgttgt ttgtttatct ctcaaaacaa acaggtccta aaatggtccc 180gggcgtccaa tgtgtcgtca agtagtgtta agctaaatcg acatttcttt gtgggttgtg 240tggaaggtgt tccttttcct taagttgtta gttgtgcaag gtgttcctta gagcatctcc 300aataggacct ataatggatt ctattttgaa ttataagact ctaacaacaa aagcatactt 360taatggggat tctattttac aaaaaaatat caaatgatta tatggtcgat tcctcgggtc 420ctaaatatag tatctcatat aatagagctc tatcctcatt ttatatacta tttttaagtt 480tttatttact aaataacatg atttattttc taatactatg aactcaacta ttagagctgt 540aaacgttttt gtggtactaa acactttaaa tcaggtccta ttttaatttg aaggacttaa 600atataagact tctggttaga gatgctctta gcgagtgttt gtgcatgatt gctatttagt 660ctttgtggat tgtggaaggt gttacttttc ctcaagttgt tagttgtgca aggtgtttct 720tagagcatct ctaacaggag ccttaacgga atctattttg aagtatagta ctttaacacc 780aaaaacatac tttaataggg gtcctatttt acaaaaaaat tatcaaatga ttataaggtc 840cactcctcgg gtcctaaata taatatctca tatactagag ctctatcctc attttatata 900ctatccctag gtttttattc cctaaataac atgatttatt tcctaatact aagatatagg 960gctcaactat tggagttgca aatgtttttt ggcactaaac actttatatc aggtcctatt 1020ttaattttaa tttgaaggac tcaaatatag gacttctcgt tagagatgct cttagcgagt 1080gtttgtgcat gattgctatt tatgtctgta gtttagttgg gggctttaat atgtttagtt 1140gaagttctag tattttttag gttctccact ctttggatta tgacaacgac cactatccaa 1200gcagtctttg agtgcaaacg cgcgagcaaa ctatctgatc tattaaatta tgatccaacc 1260gttatgtcat attgaagact taaacccttt caccaccagc ccaagtatct ttatgaaaaa 1320ccctaacaaa ccacaattgc atctatggtt ggattataat ttaacgtatc agatggttcg 1380cttgcatgct tacatatcta gaaactgttt gcataacagt cgttctcttt ggttatataa 1440tgctttagta atcatcagcc aagtgtaaac aaatggtaca aactagtagt gaacacatcc 1500tccctaccta tctctagggg tgtcatagta aattctatgt cttatttgtc cgcgattgaa 1560gaaaaatgac aaaaagatct gacattcgaa taaacatctg tttccactcc tacctatctg 1620acctcctatt tcaaactcca ctttgtaaca cggtacaaaa tcactcccta cctatctgac 1680ctcctatttc aaactccact cagtaaacaa tattgtctat ggtacaaaac caagtgtttt 1740atacatctat ttgcacgatc tgctcgagtc aggcatcctt gacacacaac atactccttg 1800tggctataaa tgtccaaata gagcagacct aatgggtgga ccgttgcatg acacgactta 1860tcccaagacg agcacagttc gccccattgg tcatgggggt ccgggctagt ctagcctgat 1920catcgggtca cacttaggcc acaggtgtgc cacaacggga tagcccaaca tgtccctttt 1980tgtcatgcat atatctatat tatagttagt ataatgtaaa aaaacaaaag gtatgtgtgt 2040tatgttggtt agatgtgttt aaataactct ttaaagctag caactatggt ttaaatcata 2100catatacaca tttttatttt atttttattt aaacgatatg ggccttctag gcacgtcgag 2160tgtgacgggc cagtgagatg acacattata attactggtc tagcaggccg tacctaggtc 2220tttctcgtgg gccaagacta agggttggcc cgttggctaa tctgtacggt accgatactg 2280tcctaattca tttgaacacc tgtagaagag gggaatttat aattgaggag gaatgtactc 2340atgcggtaca ccaggggaat tgttttgttg tgctcagcga tagatttcaa cgcaacggtg 2400agccagtttc actaaaaaaa gggggggggg gggggggggg ggaaggccac atcaaaggcg 2460aggtgctgac gagcagaaga tgctagcagt gacgccaagt ccagcagcta gcaatgaaag 2520ggtactcggg atttaacaat gcctagagac ggcatcatcc cctcaataat ccggtgctct 2580ctttttgttt attcaccagt tggcgtagct atatacacat gtctggtctg acgaacaaat 2640caagggatcg ctagctcggg ctagccttcc tatcactgtc atgacatgtg ctctgcctct 2700gctggttgat aagccgtgcg ccttctcgct aattctttct tgtgctagag gcgagtcaaa 2760caaacgctgc acctcgtagc ccttaatctg cgctaagggt cacatgaccc tgttccctat 2820cgctagttac caacgaccca ttccccctga cagatactta cgacgcgtcc gtacgcggca 2880ggcctcggca gttcggcatc accagcaccg gcgccggcat tcgccccctg ccagccggtt 2940cgcagattcg cagggcggag tcggccgcag ttgccgcatc ccaaacgccc gggaaccttt 3000ggggcccctc tacgagcaaa tgaagttgct gcccctggct tcgtaaagct ctgacttttg 3060atcacttgat tggcagtcgt actcctcgct cataggccga cacggccgca aagtcaacta 3120cccgctccgc catccttcaa cccccgccac gcgcctatat atgttcgcgg ccatgtccgt 3180actagtcctc caacccacaa gccacaaccc cgagctcaga tccctcgcct cgtgtcgtgt 3240ctccggtcga cgacgaccaa cagccagtgt gggccagacg gacaccgccg agctatagcg 3300cttggtgata gcaagggacg accg 332490500DNAZea mays 90agttaccaac gacccattcc ccctgacaga tacttacgac gcgtccgtac gcggcaggcc 60tcggcagttc ggcatcacca gcaccggcgc cggcattcgc cccctgccag ccggttcgca 120gattcgcagg gcggagtcgg ccgcagttgc cgcatcccaa acgcccggga acctttgggg 180cccctctacg agcaaatgaa gttgctgccc ctggcttcgt aaagctctga cttttgatca 240cttgattggc agtcgtactc ctcgctcata ggccgacacg gccgcaaagt caactacccg 300ctccgccatc cttcaacccc cgccacgcgc ctatatatgt tcgcggccat gtccgtacta 360gtcctccaac ccacaagcca caaccccgag ctcagatccc tcgcctcgtg tcgtgtctcc 420ggtcgacgac gaccaacagc cagtgtgggc cagacggaca ccgccgagct atagcgcttg 480gtgatagcaa gggacgaccg 500912025DNAZea mays 91gagcgctccg ctgccgtgcg cgcccccgcg ccggcctccc actggatcgc tccacctcat 60gctccaaatc tttattggtt tccacgttgc cccctcgccg tccccaacca tcgaccgcgc 120cgcgcccgct gccgcctccc agctcgctct atataaacac cacgtacgcg ccgaagcatc 180agcacagcca cgtacgtacg accggcttcc ggcaggtgag agaacagtga gaagcaggcg 240agcggtgaca tggcggaggg ggagttcaag cccgcggcga tgcaggtgga ggctcctgcc 300gaggcggcgg cggcgccgtc caagccgcgg ttcaggatgc ccgtcgactc cgacaacaag 360gccaccgagt tctggctctt ctccttcgcg aggccgcaca tgagcgcctt ccacatgtcg 420tggttctcct tcttctgctg cttcctctcc accttcgcgg cgccgccgct gctcccgctc 480atccgggaca cgctggggct cacggccacg gacatcggca acgccgggat cgcctccgtg 540tccggcgcgg tcttcgcgcg cgtggccatg ggcacggcgt gcgacctggt gggcccgcgc 600ctggcgtccg cggccatcat actcctcacc acgcccgccg tctactactc cgccgtcatc 660gactccgcct cgtcctacct gctcgtgcgc ttcttcacgg gcttctcgct cgcgtccttc 720gtgtccacgc agttctggat gagctccatg ttctcgccgc ccaaggtggg gctggccaac 780ggcgtcgccg gggggtgggg caacctcggc ggcggcgccg tgcagctcat catgccgctc 840gtgttcgagg ccatccgcaa ggccggggcc acgccgttca cggcgtggcg cgtcgccttc 900ttcgtcccgg gcctgctgca gacgctgtcg gccgtcgccg tgctggcgtt cggccaggac 960atgcccgacg gcaactaccg caagctgcac aggtccggcg acatgcacaa ggacagcttc 1020ggcaacgtgc tccgccacgc cgtcaccaac taccgcgcct ggatcctggc gctcacctac 1080ggatactgct tcggcgtgga gctcgccgtg gacaacatcg tcgcgcagta cttctacgac 1140cgcttcggcg tcaagctcag caccgccggc ttcatcgccg ccagcttcgg gatggccaac 1200atcgtctccc gccccggcgg cggcctcctg tcggactggc tctccagccg cttcggcatg 1260cgcggcaggc tgtggggcct gtgggtggtg cagaccatcg ggggcgtcct ctgcgtcgtg 1320ctcggcgccg tcgactactc cttcgccgcg tccgtggccg tcatgatact cttctccatg 1380ttcgtgcagg cggcctgcgg gctcaccttt ggcatcgtcc cgttcgtctc ccgaaggtcg 1440ctggggctca tctccggcat gaccggcggc ggcggcaacg tgggcgccgt gctcacgcag 1500ctcatcttct tccacggatc caagtacaag acggagacgg ggatcaagta catggggttc 1560atgatcatcg cctgcacgtt gcccatcacg ctcatctact tcccgcagtg gggcggcatg 1620ttcctggggc cgcggcccgg ggcgacggcg gaggactact acaaccggga gtggacagcg 1680cacgagtgcg acaagggttt caacaccgcg agcgtacgct ttgcggagaa cagcgtgcgg 1740gaagggggac gctcgggcag

ccagtccaag cacactactg tgcccgtcga gtcctcgccg 1800gccgacgtgt gaaacacaca caagcatacg gtactgcccg tataatcagc ggtccctccc 1860gtgtcagcaa atcatatgta gtgttcctaa gtcgtgatga ctccgtacgt gtggtaattt 1920ctgtgtgaag gaaaaaccgg gggtgaattt cagcgaggag tgacattata agcagggctc 1980gtttgcataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 202592520PRTZea mays 92Met Ala Glu Gly Glu Phe Lys Pro Ala Ala Met Gln Val Glu Ala Pro 1 5 10 15 Ala Glu Ala Ala Ala Ala Pro Ser Lys Pro Arg Phe Arg Met Pro Val 20 25 30 Asp Ser Asp Asn Lys Ala Thr Glu Phe Trp Leu Phe Ser Phe Ala Arg 35 40 45 Pro His Met Ser Ala Phe His Met Ser Trp Phe Ser Phe Phe Cys Cys 50 55 60 Phe Leu Ser Thr Phe Ala Ala Pro Pro Leu Leu Pro Leu Ile Arg Asp 65 70 75 80 Thr Leu Gly Leu Thr Ala Thr Asp Ile Gly Asn Ala Gly Ile Ala Ser 85 90 95 Val Ser Gly Ala Val Phe Ala Arg Val Ala Met Gly Thr Ala Cys Asp 100 105 110 Leu Val Gly Pro Arg Leu Ala Ser Ala Ala Ile Ile Leu Leu Thr Thr 115 120 125 Pro Ala Val Tyr Tyr Ser Ala Val Ile Asp Ser Ala Ser Ser Tyr Leu 130 135 140 Leu Val Arg Phe Phe Thr Gly Phe Ser Leu Ala Ser Phe Val Ser Thr 145 150 155 160 Gln Phe Trp Met Ser Ser Met Phe Ser Pro Pro Lys Val Gly Leu Ala 165 170 175 Asn Gly Val Ala Gly Gly Trp Gly Asn Leu Gly Gly Gly Ala Val Gln 180 185 190 Leu Ile Met Pro Leu Val Phe Glu Ala Ile Arg Lys Ala Gly Ala Thr 195 200 205 Pro Phe Thr Ala Trp Arg Val Ala Phe Phe Val Pro Gly Leu Leu Gln 210 215 220 Thr Leu Ser Ala Val Ala Val Leu Ala Phe Gly Gln Asp Met Pro Asp 225 230 235 240 Gly Asn Tyr Arg Lys Leu His Arg Ser Gly Asp Met His Lys Asp Ser 245 250 255 Phe Gly Asn Val Leu Arg His Ala Val Thr Asn Tyr Arg Ala Trp Ile 260 265 270 Leu Ala Leu Thr Tyr Gly Tyr Cys Phe Gly Val Glu Leu Ala Val Asp 275 280 285 Asn Ile Val Ala Gln Tyr Phe Tyr Asp Arg Phe Gly Val Lys Leu Ser 290 295 300 Thr Ala Gly Phe Ile Ala Ala Ser Phe Gly Met Ala Asn Ile Val Ser 305 310 315 320 Arg Pro Gly Gly Gly Leu Leu Ser Asp Trp Leu Ser Ser Arg Phe Gly 325 330 335 Met Arg Gly Arg Leu Trp Gly Leu Trp Val Val Gln Thr Ile Gly Gly 340 345 350 Val Leu Cys Val Val Leu Gly Ala Val Asp Tyr Ser Phe Ala Ala Ser 355 360 365 Val Ala Val Met Ile Leu Phe Ser Met Phe Val Gln Ala Ala Cys Gly 370 375 380 Leu Thr Phe Gly Ile Val Pro Phe Val Ser Arg Arg Ser Leu Gly Leu 385 390 395 400 Ile Ser Gly Met Thr Gly Gly Gly Gly Asn Val Gly Ala Val Leu Thr 405 410 415 Gln Leu Ile Phe Phe His Gly Ser Lys Tyr Lys Thr Glu Thr Gly Ile 420 425 430 Lys Tyr Met Gly Phe Met Ile Ile Ala Cys Thr Leu Pro Ile Thr Leu 435 440 445 Ile Tyr Phe Pro Gln Trp Gly Gly Met Phe Leu Gly Pro Arg Pro Gly 450 455 460 Ala Thr Ala Glu Asp Tyr Tyr Asn Arg Glu Trp Thr Ala His Glu Cys 465 470 475 480 Asp Lys Gly Phe Asn Thr Ala Ser Val Arg Phe Ala Glu Asn Ser Val 485 490 495 Arg Glu Gly Gly Arg Ser Gly Ser Gln Ser Lys His Thr Thr Val Pro 500 505 510 Val Glu Ser Ser Pro Ala Asp Val 515 520 9349597DNAArtificial SeqeunceVector 93gtcttgctcg actctagagc tcgttcctcg aggcctcgag gcctcgagga acggtacctg 60cggggaagct tacaataatg tgtgttgtta agtcttgttg cctgtcatcg tctgactgac 120tttcgtcata aatcccggcc tccgtaaccc agctttgggc aagctcacgg atttgatccg 180gcggaacggg aatatcgaga tgccgggctg aacgctgcag ttccagcttt ccctttcggg 240acaggtactc cagctgattg attatctgct gaagggtctt ggttccacct cctggcacaa 300tgcgaatgat tacttgagcg cgatcgggca tccaattttc tcccgtcagg tgcgtggtca 360agtgctacaa ggcacctttc agtaacgagc gaccgtcgat ccgtcgccgg gatacggaca 420aaatggagcg cagtagtcca tcgagggcgg cgaaagcctc gccaaaagca atacgttcat 480ctcgcacagc ctccagatcc gatcgagggt cttcggcgta ggcagataga agcatggata 540cattgcttga gagtattccg atggactgaa gtatggcttc catcttttct cgtgtgtctg 600catctatttc gagaaagccc ccgatgcggc gcaccgcaac gcgaattgcc atactatccg 660aaagtcccag caggcgcgct tgataggaaa aggtttcata ctcggccgat cgcagacggg 720cactcacgac cttgaaccct tcaactttca gggatcgatg ctggttgatg gtagtctcac 780tcgacgtggc tctggtgtgt tttgacatag cttcctccaa agaaagcgga aggtctggat 840actccagcac gaaatgtgcc cgggtagacg gatggaagtc tagccctgct caatatgaaa 900tcaacagtac atttacagtc aatactgaat atacttgcta catttgcaat tgtcttataa 960cgaatgtgaa ataaaaatag tgtaacaacg cttttactca tcgataatca caaaaacatt 1020tatacgaaca aaaatacaaa tgcactccgg tttcacagga taggcgggat cagaatatgc 1080aacttttgac gttttgttct ttcaaagggg gtgctggcaa aaccaccgca ctcatgggcc 1140tttgcgctgc tttggcaaat gacggtaaac gagtggccct ctttgatgcc gacgaaaacc 1200ggcctctgac gcgatggaga gaaaacgcct tacaaagcag tactgggatc ctcgctgtga 1260agtctattcc gccgacgaaa tgccccttct tgaagcagcc tatgaaaatg ccgagctcga 1320aggatttgat tatgcgttgg ccgatacgcg tggcggctcg agcgagctca acaacacaat 1380catcgctagc tcaaacctgc ttctgatccc caccatgcta acgccgctcg acatcgatga 1440ggcactatct acctaccgct acgtcatcga gctgctgttg agtgaaaatt tggcaattcc 1500tacagctgtt ttgcgccaac gcgtcccggt cggccgattg acaacatcgc aacgcaggat 1560gtcagagacg ctagagagcc ttccagttgt accgtctccc atgcatgaaa gagatgcatt 1620tgccgcgatg aaagaacgcg gcatgttgca tcttacatta ctaaacacgg gaactgatcc 1680gacgatgcgc ctcatagaga ggaatcttcg gattgcgatg gaggaagtcg tggtcatttc 1740gaaactgatc agcaaaatct tggaggcttg aagatggcaa ttcgcaagcc cgcattgtcg 1800gtcggcgaag cacggcggct tgctggtgct cgacccgaga tccaccatcc caacccgaca 1860cttgttcccc agaagctgga cctccagcac ttgcctgaaa aagccgacga gaaagaccag 1920caacgtgagc ctctcgtcgc cgatcacatt tacagtcccg atcgacaact taagctaact 1980gtggatgccc ttagtccacc tccgtccccg aaaaagctcc aggtttttct ttcagcgcga 2040ccgcccgcgc ctcaagtgtc gaaaacatat gacaacctcg ttcggcaata cagtccctcg 2100aagtcgctac aaatgatttt aaggcgcgcg ttggacgatt tcgaaagcat gctggcagat 2160ggatcatttc gcgtggcccc gaaaagttat ccgatccctt caactacaga aaaatccgtt 2220ctcgttcaga cctcacgcat gttcccggtt gcgttgctcg aggtcgctcg aagtcatttt 2280gatccgttgg ggttggagac cgctcgagct ttcggccaca agctggctac cgccgcgctc 2340gcgtcattct ttgctggaga gaagccatcg agcaattggt gaagagggac ctatcggaac 2400ccctcaccaa atattgagtg taggtttgag gccgctggcc gcgtcctcag tcaccttttg 2460agccagataa ttaagagcca aatgcaattg gctcaggctg ccatcgtccc cccgtgcgaa 2520acctgcacgt ccgcgtcaaa gaaataaccg gcacctcttg ctgtttttat cagttgaggg 2580cttgacggat ccgcctcaag tttgcggcgc agccgcaaaa tgagaacatc tatactcctg 2640tcgtaaacct cctcgtcgcg tactcgactg gcaatgagaa gttgctcgcg cgatagaacg 2700tcgcggggtt tctctaaaaa cgcgaggaga agattgaact cacctgccgt aagtttcacc 2760tcaccgccag cttcggacat caagcgacgt tgcctgagat taagtgtcca gtcagtaaaa 2820caaaaagacc gtcggtcttt ggagcggaca acgttggggc gcacgcgcaa ggcaacccga 2880atgcgtgcaa gaaactctct cgtactaaac ggcttagcga taaaatcact tgctcctagc 2940tcgagtgcaa caactttatc cgtctcctca aggcggtcgc cactgataat tatgattgga 3000atatcagact ttgccgccag atttcgaacg atctcaagcc catcttcacg acctaaattt 3060agatcaacaa ccacgacatc gaccgtcgcg gaagagagta ctctagtgaa ctgggtgctg 3120tcggctaccg cggtcacttt gaaggcgtgg atcgtaaggt attcgataat aagatgccgc 3180atagcgacat cgtcatcgat aagaagaacg tgtttcaacg gctcaccttt caatctaaaa 3240tctgaaccct tgttcacagc gcttgagaaa ttttcacgtg aaggatgtac aatcatctcc 3300agctaaatgg gcagttcgtc agaattgcgg ctgaccgcgg atgacgaaaa tgcgaaccaa 3360gtatttcaat tttatgacaa aagttctcaa tcgttgttac aagtgaaacg cttcgaggtt 3420acagctacta ttgattaagg agatcgccta tggtctcgcc ccggcgtcgt gcgtccgccg 3480cgagccagat ctcgcctact tcataaacgt cctcataggc acggaatgga atgatgacat 3540cgatcgccgt agagagcatg tcaatcagtg tgcgatcttc caagctagca ccttgggcgc 3600tacttttgac aagggaaaac agtttcttga atccttggat tggattcgcg ccgtgtattg 3660ttgaaatcga tcccggatgt cccgagacga cttcactcag ataagcccat gctgcatcgt 3720cgcgcatctc gccaagcaat atccggtccg gccgcatacg cagacttgct tggagcaagt 3780gctcggcgct cacagcaccc agcccagcac cgttcttgga gtagagtagt ctaacatgat 3840tatcgtgtgg aatgacgagt tcgagcgtat cttctatggt gattagcctt tcctgggggg 3900ggatggcgct gatcaaggtc ttgctcattg ttgtcttgcc gcttccggta gggccacata 3960gcaacatcgt cagtcggctg acgacgcatg cgtgcagaaa cgcttccaaa tccccgttgt 4020caaaatgctg aaggatagct tcatcatcct gattttggcg tttccttcgt gtctgccact 4080ggttccacct cgaagcatca taacgggagg agacttcttt aagaccagaa acacgcgagc 4140ttggccgtcg aatggtcaag ctgacggtgc ccgagggaac ggtcggcggc agacagattt 4200gtagtcgttc accaccagga agttcagtgg cgcagagggg gttacgtggt ccgacatcct 4260gctttctcag cgcgcccgct aaaatagcga tatcttcaag atcatcataa gagacgggca 4320aaggcatctt ggtaaaaatg ccggcttggc gcacaaatgc ctctccaggt cgattgatcg 4380caatttcttc agtcttcggg tcatcgagcc attccaaaat cggcttcaga agaaagcgta 4440gttgcggatc cacttccatt tacaatgtat cctatctcta agcggaaatt tgaattcatt 4500aagagcggcg gttcctcccc cgcgtggcgc cgccagtcag gcggagctgg taaacaccaa 4560agaaatcgag gtcccgtgct acgaaaatgg aaacggtgtc accctgattc ttcttcaggg 4620ttggcggtat gttgatggtt gccttaaggg ctgtctcagt tgtctgctca ccgttatttt 4680gaaagctgtt gaagctcatc ccgccacccg agctgccggc gtaggtgcta gctgcctgga 4740aggcgccttg aacaacactc aagagcatag ctccgctaaa acgctgccag aagtggctgt 4800cgaccgagcc cggcaatcct gagcgaccga gttcgtccgc gcttggcgat gttaacgaga 4860tcatcgcatg gtcaggtgtc tcggcgcgat cccacaacac aaaaacgcgc ccatctccct 4920gttgcaagcc acgctgtatt tcgccaacaa cggtggtgcc acgatcaaga agcacgatat 4980tgttcgttgt tccacgaata tcctgaggca agacacactt tacatagcct gccaaatttg 5040tgtcgattgc ggtttgcaag atgcacggaa ttattgtccc ttgcgttacc ataaaatcgg 5100ggtgcggcaa gagcgtggcg ctgctgggct gcagctcggt gggtttcata cgtatcgaca 5160aatcgttctc gccggacact tcgccattcg gcaaggagtt gtcgtcacgc ttgccttctt 5220gtcttcggcc cgtgtcgccc tgaatggcgc gtttgctgac cccttgatcg ccgctgctat 5280atgcaaaaat cggtgtttct tccggccgtg gctcatgccg ctccggttcg cccctcggcg 5340gtagaggagc agcaggctga acagcctctt gaaccgctgg aggatccggc ggcacctcaa 5400tcggagctgg atgaaatggc ttggtgtttg ttgcgatcaa agttgacggc gatgcgttct 5460cattcacctt cttttggcgc ccacctagcc aaatgaggct taatgataac gcgagaacga 5520cacctccgac gatcaatttc tgagaccccg aaagacgccg gcgatgtttg tcggagacca 5580gggatccaga tgcatcaacc tcatgtgccg cttgctgact atcgttattc atcccttcgc 5640ccccttcagg acgcgtttca catcgggcct caccgtgccc gtttgcggcc tttggccaac 5700gggatcgtaa gcggtgttcc agatacatag tactgtgtgg ccatccctca gacgccaacc 5760tcgggaaacc gaagaaatct cgacatcgct ccctttaact gaatagttgg caacagcttc 5820cttgccatca ggattgatgg tgtagatgga gggtatgcgt acattgcccg gaaagtggaa 5880taccgtcgta aatccattgt cgaagacttc gagtggcaac agcgaacgat cgccttgggc 5940gacgtagtgc caattactgt ccgccgcacc aagggctgtg acaggctgat ccaataaatt 6000ctcagctttc cgttgatatt gtgcttccgc gtgtagtctg tccacaacag ccttctgttg 6060tgcctccctt cgccgagccg ccgcatcgtc ggcggggtag gcgaattgga cgctgtaata 6120gagatcgggc tgctctttat cgaggtggga cagagtcttg gaacttatac tgaaaacata 6180acggcgcatc ccggagtcgc ttgcggttag cacgattact ggctgaggcg tgaggacctg 6240gcttgccttg aaaaatagat aatttccccg cggtagggct gctagatctt tgctatttga 6300aacggcaacc gctgtcaccg tttcgttcgt ggcgaatgtt acgaccaaag tagctccaac 6360cgccgtcgag aggcgcacca cttgatcggg attgtaagcc aaataacgca tgcgcggatc 6420tagcttgccc gccattggag tgtcttcagc ctccgcacca gtcgcagcgg caaataaaca 6480tgctaaaatg aaaagtgctt ttctgatcat ggttcgctgt ggcctacgtt tgaaacggta 6540tcttccgatg tctgatagga ggtgacaacc agacctgccg ggttggttag tctcaatctg 6600ccgggcaagc tggtcacctt ttcgtagcga actgtcgcgg tccacgtact caccacaggc 6660attttgccgt caacgacgag ggtcctttta tagcgaattt gctgcgtgct tggagttaca 6720tcatttgaag cgatgtgctc gacctccacc ctgccgcgtt tgccaagaat gacttgaggc 6780gaactgggat tgggatagtt gaagaattgc tggtaatcct ggcgcactgt tggggcactg 6840aagttcgata ccaggtcgta ggcgtactga gcggtgtcgg catcataact ctcgcgcagg 6900cgaacgtact cccacaatga ggcgttaacg acggcctcct cttgagttgc aggcaatcgc 6960gagacagaca cctcgctgtc aacggtgccg tccggccgta tccatagata tacgggcaca 7020agcctgctca acggcaccat tgtggctata gcgaacgctt gagcaacatt tcccaaaatc 7080gcgatagctg cgacagctgc aatgagtttg gagagacgtc gcgccgattt cgctcgcgcg 7140gtttgaaagg cttctacttc cttatagtgc tcggcaaggc tttcgcgcgc cactagcatg 7200gcatattcag gccccgtcat agcgtccacc cgaattgccg agctgaagat ctgacggagt 7260aggctgccat cgccccacat tcagcgggaa gatcgggcct ttgcagctcg ctaatgtgtc 7320gtttgtctgg cagccgctca aagcgacaac taggcacagc aggcaatact tcatagaatt 7380ctccattgag gcgaattttt gcgcgaccta gcctcgctca acctgagcga agcgacggta 7440caagctgctg gcagattggg ttgcgccgct ccagtaactg cctccaatgt tgccggcgat 7500cgccggcaaa gcgacaatga gcgcatcccc tgtcagaaaa aacatatcga gttcgtaaag 7560accaatgatc ttggccgcgg tcgtaccggc gaaggtgatt acaccaagca taagggtgag 7620cgcagtcgct tcggttagga tgacgatcgt tgccacgagg tttaagagga gaagcaagag 7680accgtaggtg ataagttgcc cgatccactt agctgcgatg tcccgcgtgc gatcaaaaat 7740atatccgacg aggatcagag gcccgatcgc gagaagcact ttcgtgagaa ttccaacggc 7800gtcgtaaact ccgaaggcag accagagcgt gccgtaaagg acccactgtg ccccttggaa 7860agcaaggatg tcctggtcgt tcatcggacc gatttcggat gcgattttct gaaaaacggc 7920ctgggtcacg gcgaacattg tatccaactg tgccggaaca gtctgcagag gcaagccggt 7980tacactaaac tgctgaacaa agtttgggac cgtcttttcg aagatggaaa ccacatagtc 8040ttggtagtta gcctgcccaa caattagagc aacaacgatg gtgaccgtga tcacccgagt 8100gataccgcta cgggtatcga cttcgccgcg tatgactaaa ataccctgaa caataatcca 8160aagagtgaca caggcgatca atggcgcact caccgcctcc tggatagtct caagcatcga 8220gtccaagcct gtcgtgaagg ctacatcgaa gatcgtatga atggccgtaa acggcgccgg 8280aatcgtgaaa ttcatcgatt ggacctgaac ttgactggtt tgtcgcataa tgttggataa 8340aatgagctcg cattcggcga ggatgcgggc ggatgaacaa atcgcccagc cttaggggag 8400ggcaccaaag atgacagcgg tcttttgatg ctccttgcgt tgagcggccg cctcttccgc 8460ctcgtgaagg ccggcctgcg cggtagtcat cgttaatagg cttgtcgcct gtacattttg 8520aatcattgcg tcatggatct gcttgagaag caaaccattg gtcacggttg cctgcatgat 8580attgcgagat cgggaaagct gagcagacgt atcagcattc gccgtcaagc gtttgtccat 8640cgtttccaga ttgtcagccg caatgccagc gctgtttgcg gaaccggtga tctgcgatcg 8700caacaggtcc gcttcagcat cactacccac gactgcacga tctgtatcgc tggtgatcgc 8760acgtgccgtg gtcgacattg gcattcgcgg cgaaaacatt tcattgtcta ggtccttcgt 8820cgaaggatac tgatttttct ggttgagcga agtcagtagt ccagtaacgc cgtaggccga 8880cgtcaacatc gtaaccatcg ctatagtctg agtgagattc tccgcagtcg cgagcgcagt 8940cgcgagcgtc tcagcctccg ttgccgggtc gctaacaaca aactgcgccc gcgcgggctg 9000aatatataga aagctgcagg tcaaaactgt tgcaataagt tgcgtcgtct tcatcgtttc 9060ctaccttatc aatcttctgc ctcgtggtga cgggccatga attcgctgag ccagccagat 9120gagttgcctt cttgtgcctc gcgtagtcga gttgcaaagc gcaccgtgtt ggcacgcccc 9180gaaagcacgg cgacatattc acgcatatcc cgcagatcaa attcgcagat gacgcttcca 9240ctttctcgtt taagaagaaa cttacggctg ccgaccgtca tgtcttcacg gatcgcctga 9300aattcctttt cggtacattt cagtccatcg acataagccg atcgatctgc ggttggtgat 9360ggatagaaaa tcttcgtcat acattgcgca accaagctgg ctcctagcgg cgattccaga 9420acatgctctg gttgctgcgt tgccagtatt agcatcccgt tgttttttcg aacggtcagg 9480aggaatttgt cgacgacagt cgaaaattta gggtttaaca aataggcgcg aaactcatcg 9540cagctcatca caaaacggcg gccgtcgatc atggctccaa tccgatgcag gagatatgct 9600gcagcgggag cgcatacttc ctcgtattcg agaagatgcg tcatgtcgaa gccggtaatc 9660gacggatcta actttacttc gtcaacttcg ccgtcaaatg cccagccaag cgcatggccc 9720cggcaccagc gttggagccg cgctcctgcg ccttcggcgg gcccatgcaa caaaaattca 9780cgtaaccccg cgattgaacg catttgtgga tcaaacgaga gctgacgatg gataccacgg 9840accagacggc ggttctcttc cggagaaatc ccaccccgac catcactctc gatgagagcc 9900acgatccatt cgcgcagaaa atcgtgtgag gctgctgtgt tttctaggcc acgcaacggc 9960gccaacccgc tgggtgtgcc tctgtgaagt gccaaatatg ttcctcctgt ggcgcgaacc 10020agcaattcgc caccccggtc cttgtcaaag aacacgaccg tacctgcacg gtcgaccatg 10080ctctgttcga gcatggctag aacaaacatc atgagcgtcg tcttacccct cccgataggc 10140ccgaatattg ccgtcatgcc aacatcgtgc tcatgcggga tatagtcgaa aggcgttccg 10200ccattggtac gaaatcgggc aatcgcgttg ccccagtggc ctgagctggc gccctctgga 10260aagttttcga aagagacaaa ccctgcgaaa ttgcgtgaag tgattgcgcc agggcgtgtg 10320cgccacttaa aattccccgg caattgggac caataggccg cttccatacc aataccttct 10380tggacaacca cggcacctgc atccgccatt cgtgtccgag cccgcgcgcc cctgtcccca 10440agactattga gatcgtctgc atagacgcaa aggctcaaat gatgtgagcc cataacgaat 10500tcgttgctcg caagtgcgtc ctcagcctcg gataatttgc cgatttgagt cacggcttta 10560tcgccggaac tcagcatctg gctcgatttg aggctaagtt tcgcgtgcgc ttgcgggcga 10620gtcaggaacg aaaaactctg cgtgagaaca agtggaaaat cgagggatag cagcgcgttg 10680agcatgcccg gccgtgtttt tgcagggtat tcgcgaaacg aatagatgga tccaacgtaa 10740ctgtcttttg gcgttctgat ctcgagtcct cgcttgccgc aaatgactct gtcggtataa 10800atcgaagcgc cgagtgagcc gctgacgacc ggaaccggtg tgaaccgacc agtcatgatc 10860aaccgtagcg cttcgccaat ttcggtgaag agcacaccct gcttctcgcg gatgccaaga 10920cgatgcaggc catacgcttt aagagagcca gcgacaacat gccaaagatc ttccatgttc 10980ctgatctggc ccgtgagatc gttttccctt tttccgctta gcttggtgaa cctcctcttt 11040accttcccta aagccgcctg tgggtagaca atcaacgtaa ggaagtgttc attgcggagg 11100agttggccgg agagcacgcg ctgttcaaaa gcttcgttca ggctagcggc gaaaacacta 11160cggaagtgtc gcggcgccga tgatggcacg tcggcatgac gtacgaggtg agcatatatt 11220gacacatgat catcagcgat attgcgcaac agcgtgttga acgcacgaca acgcgcattg 11280cgcatttcag tttcctcaag ctcgaatgca acgccatcaa ttctcgcaat ggtcatgatc

11340gatccgtctt caagaaggac gatatggtcg ctgaggtggc caatataagg gagatagatc 11400tcaccggatc tttcggtcgt tccactcgcg ccgagcatca caccattcct ctccctcgtg 11460ggggaaccct aattggattt gggctaacag tagcgccccc ccaaactgca ctatcaatgc 11520ttcttcccgc ggtccgcaaa aatagcagga cgacgctcgc cgcattgtag tctcgctcca 11580cgatgagccg ggctgcaaac cataacggca cgagaacgac ttcgtagagc gggttctgaa 11640cgataacgat gacaaagccg gcgaacatca tgaataaccc tgccaatgtc agtggcaccc 11700caagaaacaa tgcgggccgt gtggctgcga ggtaaagggt cgattcttcc aaacgatcag 11760ccatcaacta ccgccagtga gcgtttggcc gaggaagctc gccccaaaca tgataacaat 11820gccgccgacg acgccggcaa ccagcccaag cgaagcccgc ccgaacatcc aggagatccc 11880gatagcgaca atgccgagaa cagcgagtga ctggccgaac ggaccaagga taaacgtgca 11940tatattgtta accattgtgg cggggtcagt gccgccaccc gcagattgcg ctgcggcggg 12000tccggatgag gaaatgctcc atgcaattgc accgcacaag cttggggcgc agctcgatat 12060cacgcgcatc atcgcattcg agagcgagag gcgatttaga tgtaaacggt atctctcaaa 12120gcatcgcatc aatgcgcacc tccttagtat aagtcgaata agacttgatt gtcgtctgcg 12180gatttgccgt tgtcctggtg tggcggtggc ggagcgatta aaccgccagc gccatcctcc 12240tgcgagcggc gctgatatga cccccaaaca tcccacgtct cttcggattt tagcgcctcg 12300tgatcgtctt ttggaggctc gattaacgcg ggcaccagcg attgagcagc tgtttcaact 12360tttcgcacgt agccgtttgc aaaaccgccg atgaaattac cggtgttgta agcggagatc 12420gcccgacgaa gcgcaaattg cttctcgtca atcgtttcgc cgcctgcata acgacttttc 12480agcatgtttg cagcggcaga taatgatgtg cacgcctgga gcgcaccgtc aggtgtcaga 12540ccgagcatag aaaaatttcg agagtttatt tgcatgaggc caacatccag cgaatgccgt 12600gcatcgagac ggtgcctgac gacttgggtt gcttggctgt gatcttgcca gtgaagcgtt 12660tcgccggtcg tgttgtcatg aatcgctaaa ggatcaaagc gactctccac cttagctatc 12720gccgcaagcg tagatgtcgc aactgatggg gcacacttgc gagcaacatg gtcaaactca 12780gcagatgaga gtggcgtggc aaggctcgac gaacagaagg agaccatcaa ggcaagagaa 12840agcgaccccg atctcttaag cataccttat ctccttagct cgcaactaac accgcctctc 12900ccgttggaag aagtgcgttg ttttatgttg aagattatcg ggagggtcgg ttactcgaaa 12960attttcaatt gcttctttat gatttcaatt gaagcgagaa acctcgcccg gcgtcttgga 13020acgcaacatg gaccgagaac cgcgcatcca tgactaagca accggatcga cctattcagg 13080ccgcagttgg tcaggtcagg ctcagaacga aaatgctcgg cgaggttacg ctgtctgtaa 13140acccattcga tgaacgggaa gcttccttcc gattgctctt ggcaggaata ttggcccatg 13200cctgcttgcg ctttgcaaat gctcttatcg cgttggtatc atatgccttg tccgccagca 13260gaaacgcact ctaagcgatt atttgtaaaa atgtttcggt catgcggcgg tcatgggctt 13320gacccgctgt cagcgcaaga cggatcggtc aaccgtcggc atcgacaaca gcgtgaatct 13380tggtggtcaa accgccacgg gaacgtccca tacagccatc gtcttgatcc cgctgtttcc 13440cgtcgccgca tgttggtgga cgcggacaca ggaactgtca atcatgacga cattctatcg 13500aaagccttgg aaatcacact cagaatatga tcccagacgt ctgcctcacg ccatcgtaca 13560aagcgattgt agcaggttgt acaggaaccg tatcgatcag gaacgtctgc ccagggcggg 13620cccgtccgga agcgccacaa gatgacattg atcacccgcg tcaacgcgcg gcacgcgacg 13680cggcttattt gggaacaaag gactgaacaa cagtccattc gaaatcggtg acatcaaagc 13740ggggacgggt tatcagtggc ctccaagtca agcctcaatg aatcaaaatc agaccgattt 13800gcaaacctga tttatgagtg tgcggcctaa atgatgaaat cgtccttcta gatcgcctcc 13860gtggtgtagc aacacctcgc agtatcgccg tgctgacctt ggccagggaa ttgactggca 13920agggtgcttt cacatgaccg ctcttttggc cgcgatagat gatttcgttg ctgctttggg 13980cacgtagaag gagagaagtc atatcggaga aattcctcct ggcgcgagag cctgctctat 14040cgcgacggca tcccactgtc gggaacagac cggatcattc acgaggcgaa agtcgtcaac 14100acatgcgtta taggcatctt cccttgaagg atgatcttgt tgctgccaat ctggaggtgc 14160ggcagccgca ggcagatgcg atctcagcgc aacttgcggc aaaacatctc actcacctga 14220aaaccactag cgagtctcgc gatcagacga aggcctttta cttaacgaca caatatccga 14280tgtctgcatc acaggcgtcg ctatcccagt caatactaaa gcggtgcagg aactaaagat 14340tactgatgac ttaggcgtgc cacgaggcct gagacgacgc gcgtagacag ttttttgaaa 14400tcattatcaa agtgatggcc tccgctgaag cctatcacct ctgcgccggt ctgtcggaga 14460gatgggcaag cattattacg gtcttcgcgc ccgtacatgc attggacgat tgcagggtca 14520atggatctga gatcatccag aggattgccg cccttacctt ccgtttcgag ttggagccag 14580cccctaaatg agacgacata gtcgacttga tgtgacaatg ccaagagaga gatttgctta 14640acccgatttt tttgctcaag cgtaagccta ttgaagcttg ccggcatgac gtccgcgccg 14700aaagaatatc ctacaagtaa aacattctgc acaccgaaat gcttggtgta gacatcgatt 14760atgtgaccaa gatccttagc agtttcgctt ggggaccgct ccgaccagaa ataccgaagt 14820gaactgacgc caatgacagg aatcccttcc gtctgcagat aggtaccatc gatagatctg 14880ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 14940ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 15000gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 15060tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 15120gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 15180ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 15240gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 15300ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 15360cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 15420ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 15480accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 15540catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 15600gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 15660tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 15720agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 15780actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 15840gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 15900aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 15960gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 16020aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 16080atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 16140gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 16200atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 16260ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 16320cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 16380agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggggg gggggggggg 16440gggttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag 16500ctcgctttca gcacctgtcg tttcctttct tttcagaggg tattttaaat aaaaacatta 16560agttatgacg aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa 16620cccgcgaggt ccctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct 16680acatatcaca acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg 16740tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca 16800gcgacactga atacggggca acctcatgtc cccccccccc ccccccctgc aggcatcgtg 16860gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 16920gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 16980gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17040cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17100ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17160accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 17220aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 17280aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 17340caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 17400ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 17460gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 17520cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 17580aggccctttc gtcttcaaga attggtcgac gatcttgctg cgttcggata ttttcgtgga 17640gttcccgcca cagacccgga ttgaaggcga gatccagcaa ctcgcgccag atcatcctgt 17700gacggaactt tggcgcgtga tgactggcca ggacgtcggc cgaaagagcg acaagcagat 17760cacgcttttc gacagcgtcg gatttgcgat cgaggatttt tcggcgctgc gctacgtccg 17820cgaccgcgtt gagggatcaa gccacagcag cccactcgac cttctagccg acccagacga 17880gccaagggat ctttttggaa tgctgctccg tcgtcaggct ttccgacgtt tgggtggttg 17940aacagaagtc attatcgtac ggaatgccaa gcactcccga ggggaaccct gtggttggca 18000tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 18060taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 18120ttaaactgaa ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt 18180atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa 18240cgttgaagga gccactcagc aagctggtac gattgtaata cgactcacta tagggcgaat 18300tgagcgctgt ttaaacgctc ttcaactgga agagcggtta cccggaccga agcttgcatg 18360cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 18420aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 18480atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 18540atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 18600agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 18660tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 18720ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 18780tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 18840ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 18900aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg 18960acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag 19020acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg 19080gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca 19140cggcaggcgg cctcctcctc ctctcacggc acggcagcta cgggggattc ctttcccacc 19200gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 19260ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 19320cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc cctctctacc 19380ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt 19440ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac 19500ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg 19560gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat 19620agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc 19680atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc 19740tagatcggag tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta 19800tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa atatcgatct 19860aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt 19920cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta 19980gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat 20040acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat 20100gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc 20160tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt attttgatct 20220tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt 20280catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt 20340gttacttctg caggtcgact ctagaggatc tacaagtttg tacaaaaaag caggctccgc 20400ggccgccccc ttcaccatgg ctcggcagca aagcgtgcag gccttgtgtg tgctggcggc 20460gcttctcttc gccgcctccc tgccgtcgcc ggccgccgcg ggggtgcacc tctcctcgct 20520gcccaaagcg ctcgacgtca ccacctccgc caaacccggc caagtcctgc acgccggcgt 20580ggactcgctg acggtgacgt ggagcctgaa cgccacggag ccggccggcg ccgacgccgg 20640gtacaagggc gtgaaggtga agctgtgcta cgcgccggcg agccagaagg accgcgggtg 20700gcgcaagtcc gaggacgaca tcagcaagga caaggcgtgc cagttcaagg tcaccgagca 20760ggcgtacgcg gcggcggcgc ccggcagctt ccagtacgcc gtcgcccgcg acgtcccctc 20820gggctcctac tacctgcgcg ccttcgccac ggacgcgtcg ggcgccgagg tggcctacgg 20880ccagacggcg cccaccgccg ccttcgacgt cgccggcatc accggcatcc acgcctctct 20940caagatcgcc gccggcgtct tctcggcctt ctccgtcgtc gcgctcgcct tcttcttcgt 21000catcgagacc cgcaagaaga acaagtagaa gggtgggcgc gccgacccag ctttcttgta 21060caaagtggtg ttaacctaga cttgtccatc ttctggattg gccaacttaa ttaatgtatg 21120aaataaaagg atgcacacat agtgacatgc taatcactat aatgtgggca tcaaagttgt 21180gtgttatgtg taattactag ttatctgaat aaaagagaaa gagatcatcc atatttctta 21240tcctaaatga atgtcacgtg tctttataat tctttgatga accagatgca tttcattaac 21300caaatccata tacatataaa tattaatcat atataattaa tatcaattgg gttagcaaaa 21360caaatctagt ctaggtgtgt tttgcgaatt gcggccgcca ccgcggtgga gctcgaattc 21420cggtccgggt cacctttgtc caccaagatg gaactgcggc cgctcattaa ttaagtcagg 21480cgcgcctcta gttgaagaca cgttcatgtc ttcatcgtaa gaagacactc agtagtcttc 21540ggccagaatg gccatctgga ttcagcaggc ctagaaggcc atttaaatcc tgaggatctg 21600gtcttcctaa ggacccgggc ggtccgatta aactttaatt cggaccgaag cttgcatgcc 21660tgcagtgcag cgtgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa 21720gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat 21780ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat 21840atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag 21900tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt 21960tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg 22020tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta 22080gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat 22140aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa 22200actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac 22260gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac 22320ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga 22380cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg 22440gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg 22500ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt 22560tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac 22620ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccc tctctacctt 22680ctctagatcg gcgttccggt ccatgcatgg ttagggcccg gtagttctac ttctgttcat 22740gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg 22800acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct 22860gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc 22920atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg 22980tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt 23040tctagatcgg agtagaattc tgtttcaaac tacctggtgg atttattaat tttggatctg 23100tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat 23160ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg 23220ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc tagatcggag 23280tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta tgtgtgtgtc 23340atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg ataggtatac 23400atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat ctattcatat 23460gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat 23520cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc 23580ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg 23640gtgttacttc tgcaggtcga ctttaactta gcctaggatc cacacgacac catgtccccc 23700gagcgccgcc ccgtcgagat ccgcccggcc accgccgccg acatggccgc cgtgtgcgac 23760atcgtgaacc actacatcga gacctccacc gtgaacttcc gcaccgagcc gcagaccccg 23820caggagtgga tcgacgacct ggagcgcctc caggaccgct acccgtggct cgtggccgag 23880gtggagggcg tggtggccgg catcgcctac gccggcccgt ggaaggcccg caacgcctac 23940gactggaccg tggagtccac cgtgtacgtg tcccaccgcc accagcgcct cggcctcggc 24000tccaccctct acacccacct cctcaagagc atggaggccc agggcttcaa gtccgtggtg 24060gccgtgatcg gcctcccgaa cgacccgtcc gtgcgcctcc acgaggccct cggctacacc 24120gcccgcggca ccctccgcgc cgccggctac aagcacggcg gctggcacga cgtcggcttc 24180tggcagcgcg acttcgagct gccggccccg ccgcgcccgg tgcgcccggt gacgcagatc 24240tgagtcgaaa cctagacttg tccatcttct ggattggcca acttaattaa tgtatgaaat 24300aaaaggatgc acacatagtg acatgctaat cactataatg tgggcatcaa agttgtgtgt 24360tatgtgtaat tactagttat ctgaataaaa gagaaagaga tcatccatat ttcttatcct 24420aaatgaatgt cacgtgtctt tataattctt tgatgaacca gatgcatttc attaaccaaa 24480tccatataca tataaatatt aatcatatat aattaatatc aattgggtta gcaaaacaaa 24540tctagtctag gtgtgttttg cgaattgcgg ccgccaccgc ggtggagctc gaattcattc 24600cgattaatcg tggcctcttg ctcttcagga tgaagagcta tgtttaaacg tgcaagcgct 24660actagacaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca 24720atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc 24780tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga 24840gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa 24900ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg 24960atgacagagc gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc 25020agccttctta ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg 25080acataatagg aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag 25140aagtgaacgt tgacgatcgt cgaccgtacc ccgatgaatt aattcggacg tacgttctga 25200acacagctgg atacttactt gggcgattgt catacatgac atcaacaatg tacccgtttg 25260tgtaaccgtc tcttggaggt tcgtatgaca ctagtggttc ccctcagctt gcgactagat 25320gttgaggcct aacattttat tagagagcag gctagttgct tagatacatg atcttcaggc 25380cgttatctgt cagggcaagc gaaaattggc catttatgac gaccaatgcc ccgcagaagc 25440tcccatcttt gccgccatag acgccgcgcc ccccttttgg ggtgtagaac atccttttgc 25500cagatgtgga aaagaagttc gttgtcccat tgttggcaat gacgtagtag ccggcgaaag 25560tgcgagaccc atttgcgcta tatataagcc tacgatttcc gttgcgacta ttgtcgtaat 25620tggatgaact attatcgtag ttgctctcag agttgtcgta atttgatgga ctattgtcgt 25680aattgcttat ggagttgtcg tagttgcttg gagaaatgtc gtagttggat ggggagtagt 25740catagggaag acgagcttca tccactaaaa caattggcag gtcagcaagt gcctgccccg 25800atgccatcgc aagtacgagg cttagaacca ccttcaacag atcgcgcata gtcttcccca 25860gctctctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta 25920gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca 25980actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa 26040gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct 26100tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat 26160ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg 26220cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca 26280aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg 26340ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc

26400gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag 26460cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag 26520ctcgccgcgt tgtttcatca agccttacag tcaccgtaac cagcaaatca atatcactgt 26580gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt 26640cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca 26700ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtcggctt 26760gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt 26820cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc 26880cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct aatcgtatgc 26940caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg tgcgcgactc 27000ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga tcgttccatg 27060ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca tagcaagcag 27120agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc acacttctgg 27180tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga acaatgaaat 27240ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatc ttcatatgac 27300gcctaacgcc tggcacagcg gatcgcaaac ctggcgcggc ttttggcaca aaaggcgtga 27360caggtttgcg aatccgttgc tgccacttgt taaccctttt gccagatttg gtaactataa 27420tttatgttag aggcgaagtc ttgggtaaaa actggcctaa aattgctggg gatttcagga 27480aagtaaacat caccttccgg ctcgatgtct attgtagata tatgtagtgt atctacttga 27540tcgggggatc tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 27600gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 27660gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 27720tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 27780catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 27840tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 27900gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 27960atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 28020ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 28080cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 28140tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 28200gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 28260aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 28320tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 28380aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 28440aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 28500ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 28560ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 28620atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 28680atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 28740tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 28800gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 28860tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 28920gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 28980cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 29040gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggg 29100gggggggggg ggggggactt ccattgttca ttccacggac aaaaacagag aaaggaaacg 29160acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc ctttcttttc agagggtatt 29220ttaaataaaa acattaagtt atgacgaaga agaacggaaa cgccttaaac cggaaaattt 29280tcataaatag cgaaaacccg cgaggtcgcc gccccgtaag ccgccccgta acctgtcgga 29340tcaccggaaa ggacccgtaa agtgataatg attatcatct acatatcaca acgtgcgtgg 29400aggccatcaa accacgtcaa ataatcaatt atgacgcagg tatcgtatta attgatctgc 29460atcaacttaa cgtaaaaaca acttcagaca atacaaatca gcgacactga atacggggca 29520acctcatgtc cccccccccc ccccccctgc aggcatcgtg gtgtcacgct cgtcgtttgg 29580tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 29640gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 29700agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 29760aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 29820gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 29880tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 29940gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 30000tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 30060aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 30120catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 30180acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 30240tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 30300attcggagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 30360ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 30420tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 30480cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 30540agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact 30600ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc 30660tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca 30720aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct 30780cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc 30840acgaggcaga cctcagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg 30900gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg 30960gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc 31020agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc 31080cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg 31140gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg 31200gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca 31260gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg 31320cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg 31380gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc 31440ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc 31500gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa 31560agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg 31620ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc 31680atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga 31740atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca 31800tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta 31860tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact 31920atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg 31980cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg 32040tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg 32100tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg 32160atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg 32220tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg 32280ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc 32340acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt 32400gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg 32460tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg 32520cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa 32580tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga 32640ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc 32700cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt 32760ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca 32820gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa 32880cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc 32940tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat 33000aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc taggagtgcg 33060gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga tgatttgaag 33120cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc ccgggctgag 33180aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa 33240gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt 33300aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc 33360cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag 33420cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc 33480tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc 33540caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat 33600cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat 33660aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca 33720ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc 33780ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga 33840cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat 33900gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc 33960ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt 34020cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat 34080ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc 34140gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc 34200tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc 34260tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct 34320cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc 34380gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg 34440aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat 34500ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa 34560cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc 34620aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat 34680tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg 34740tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc 34800tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg 34860tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg 34920cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc 34980cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc 35040agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt 35100cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc 35160gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg 35220ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg 35280acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca 35340tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc 35400cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat 35460tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag 35520tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc 35580acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa 35640cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccactct 35700tcattaaccg ctatatcgaa aattgcttgc ggcttgttag aattgccatg acgtacctcg 35760gtgtcacggg taagattacc gataaactgg aactgattat ggctcatatc gaaagtctcc 35820ttgagaaagg agactctagt ttagctaaac attggttccg ctgtcaagaa ctttagcggc 35880taaaattttg cgggccgcga ccaaaggtgc gaggggcggc ttccgctgtg tacaaccaga 35940tatttttcac caacatcctt cgtctgctcg atgagcgggg catgacgaaa catgagctgt 36000cggagagggc aggggtttca atttcgtttt tatcagactt aaccaacggt aaggccaacc 36060cctcgttgaa ggtgatggag gccattgccg acgccctgga aactccccta cctcttctcc 36120tggagtccac cgaccttgac cgcgaggcac tcgcggagat tgcgggtcat cctttcaaga 36180gcagcgtgcc gcccggatac gaacgcatca gtgtggtttt gccgtcacat aaggcgttta 36240tcgtaaagaa atggggcgac gacacccgaa aaaagctgcg tggaaggctc tgacgccaag 36300ggttagggct tgcacttcct tctttagccg ctaaaacggc cccttctctg cgggccgtcg 36360gctcgcgcat catatcgaca tcctcaacgg aagccgtgcc gcgaatggca tcgggcgggt 36420gcgctttgac agttgttttc tatcagaacc cctacgtcgt gcggttcgat tagctgtttg 36480tcttgcaggc taaacacttt cggtatatcg tttgcctgtg cgataatgtt gctaatgatt 36540tgttgcgtag gggttactga aaagtgagcg ggaaagaaga gtttcagacc atcaaggagc 36600gggccaagcg caagctggaa cgcgacatgg gtgcggacct gttggccgcg ctcaacgacc 36660cgaaaaccgt tgaagtcatg ctcaacgcgg acggcaaggt gtggcacgaa cgccttggcg 36720agccgatgcg gtacatctgc gacatgcggc ccagccagtc gcaggcgatt atagaaacgg 36780tggccggatt ccacggcaaa gaggtcacgc ggcattcgcc catcctggaa ggcgagttcc 36840ccttggatgg cagccgcttt gccggccaat tgccgccggt cgtggccgcg ccaacctttg 36900cgatccgcaa gcgcgcggtc gccatcttca cgctggaaca gtacgtcgag gcgggcatca 36960tgacccgcga gcaatacgag gtcattaaaa gcgccgtcgc ggcgcatcga aacatcctcg 37020tcattggcgg tactggctcg ggcaagacca cgctcgtcaa cgcgatcatc aatgaaatgg 37080tcgccttcaa cccgtctgag cgcgtcgtca tcatcgagga caccggcgaa atccagtgcg 37140ccgcagagaa cgccgtccaa taccacacca gcatcgacgt ctcgatgacg ctgctgctca 37200agacaacgct gcgtatgcgc cccgaccgca tcctggtcgg tgaggtacgt ggccccgaag 37260cccttgatct gttgatggcc tggaacaccg ggcatgaagg aggtgccgcc accctgcacg 37320caaacaaccc caaagcgggc ctgagccggc tcgccatgct tatcagcatg cacccggatt 37380caccgaaacc cattgagccg ctgattggcg aggcggttca tgtggtcgtc catatcgcca 37440ggacccctag cggccgtcga gtgcaagaaa ttctcgaagt tcttggttac gagaacggcc 37500agtacatcac caaaaccctg taaggagtat ttccaatgac aacggctgtt ccgttccgtc 37560tgaccatgaa tcgcggcatt ttgttctacc ttgccgtgtt cttcgttctc gctctcgcgt 37620tatccgcgca tccggcgatg gcctcggaag gcaccggcgg cagcttgcca tatgagagct 37680ggctgacgaa cctgcgcaac tccgtaaccg gcccggtggc cttcgcgctg tccatcatcg 37740gcatcgtcgt cgccggcggc gtgctgatct tcggcggcga actcaacgcc ttcttccgaa 37800ccctgatctt cctggttctg gtgatggcgc tgctggtcgg cgcgcagaac gtgatgagca 37860ccttcttcgg tcgtggtgcc gaaatcgcgg ccctcggcaa cggggcgctg caccaggtgc 37920aagtcgcggc ggcggatgcc gtgcgtgcgg tagcggctgg acggctcgcc taatcatggc 37980tctgcgcacg atccccatcc gtcgcgcagg caaccgagaa aacctgttca tgggtggtga 38040tcgtgaactg gtgatgttct cgggcctgat ggcgtttgcg ctgattttca gcgcccaaga 38100gctgcgggcc accgtggtcg gtctgatcct gtggttcggg gcgctctatg cgttccgaat 38160catggcgaag gccgatccga agatgcggtt cgtgtacctg cgtcaccgcc ggtacaagcc 38220gtattacccg gcccgctcga ccccgttccg cgagaacacc aatagccaag ggaagcaata 38280ccgatgatcc aagcaattgc gattgcaatc gcgggcctcg gcgcgcttct gttgttcatc 38340ctctttgccc gcatccgcgc ggtcgatgcc gaactgaaac tgaaaaagca tcgttccaag 38400gacgccggcc tggccgatct gctcaactac gccgctgtcg tcgatgacgg cgtaatcgtg 38460ggcaagaacg gcagctttat ggctgcctgg ctgtacaagg gcgatgacaa cgcaagcagc 38520accgaccagc agcgcgaagt agtgtccgcc cgcatcaacc aggccctcgc gggcctggga 38580agtgggtgga tgatccatgt ggacgccgtg cggcgtcctg ctccgaacta cgcggagcgg 38640ggcctgtcgg cgttccctga ccgtctgacg gcagcgattg aagaagagcg ctcggtcttg 38700ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc 38760atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca 38820tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc 38880ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc 38940gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc 39000cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc 39060cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg 39120ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc 39180atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc 39240aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct 39300cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa 39360gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga 39420aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct gcttccctgc 39480tgttttgtgg aatatctacc gactggaaac aggcaaatgc aggaaattac tgaactgagg 39540ggacaggcga gagacgatgc caaagagcta caccgacgag ctggccgagt gggttgaatc 39600ccgcgcggcc aagaagcgcc ggcgtgatga ggctgcggtt gcgttcctgg cggtgagggc 39660ggatgtcgag gcggcgttag cgtccggcta tgcgctcgtc accatttggg agcacatgcg 39720ggaaacgggg aaggtcaagt tctcctacga gacgttccgc tcgcacgcca ggcggcacat 39780caaggccaag cccgccgatg tgcccgcacc gcaggccaag gctgcggaac ccgcgccggc 39840acccaagacg ccggagccac ggcggccgaa gcaggggggc aaggctgaaa agccggcccc 39900cgctgcggcc ccgaccggct tcaccttcaa cccaacaccg gacaaaaagg atctactgta 39960atggcgaaaa ttcacatggt tttgcagggc aagggcgggg tcggcaagtc ggccatcgcc 40020gcgatcattg cgcagtacaa gatggacaag gggcagacac ccttgtgcat cgacaccgac 40080ccggtgaacg cgacgttcga gggctacaag gccctgaacg tccgccggct gaacatcatg 40140gccggcgacg aaattaactc gcgcaacttc gacaccctgg tcgagctgat tgcgccgacc 40200aaggatgacg tggtgatcga caacggtgcc agctcgttcg tgcctctgtc gcattacctc 40260atcagcaacc aggtgccggc tctgctgcaa gaaatggggc atgagctggt catccatacc 40320gtcgtcaccg gcggccaggc tctcctggac acggtgagcg gcttcgccca gctcgccagc 40380cagttcccgg ccgaagcgct tttcgtggtc tggctgaacc cgtattgggg gcctatcgag 40440catgagggca agagctttga gcagatgaag gcgtacacgg ccaacaaggc ccgcgtgtcg 40500tccatcatcc agattccggc cctcaaggaa gaaacctacg gccgcgattt cagcgacatg 40560ctgcaagagc ggctgacgtt cgaccaggcg ctggccgatg aatcgctcac gatcatgacg 40620cggcaacgcc tcaagatcgt gcggcgcggc ctgtttgaac agctcgacgc ggcggccgtg 40680ctatgagcga ccagattgaa gagctgatcc gggagattgc ggccaagcac ggcatcgccg 40740tcggccgcga cgacccggtg ctgatcctgc ataccatcaa cgcccggctc atggccgaca 40800gtgcggccaa gcaagaggaa atccttgccg cgttcaagga agagctggaa gggatcgccc 40860atcgttgggg cgaggacgcc aaggccaaag cggagcggat gctgaacgcg gccctggcgg 40920ccagcaagga cgcaatggcg aaggtaatga aggacagcgc cgcgcaggcg gccgaagcga 40980tccgcaggga aatcgacgac ggccttggcc gccagctcgc ggccaaggtc gcggacgcgc 41040ggcgcgtggc gatgatgaac atgatcgccg gcggcatggt gttgttcgcg gccgccctgg 41100tggtgtgggc ctcgttatga atcgcagagg cgcagatgaa aaagcccggc gttgccgggc 41160tttgtttttg cgttagctgg gcttgtttga caggcccaag ctctgactgc gcccgcgctc 41220gcgctcctgg gcctgtttct tctcctgctc ctgcttgcgc atcagggcct ggtgccgtcg 41280ggctgcttca cgcatcgaat cccagtcgcc ggccagctcg ggatgctccg cgcgcatctt 41340gcgcgtcgcc agttcctcga tcttgggcgc gtgaatgccc atgccttcct tgatttcgcg 41400caccatgtcc agccgcgtgt gcagggtctg caagcgggct tgctgttggg cctgctgctg

41460ctgccaggcg gcctttgtac gcggcaggga cagcaagccg ggggcattgg actgtagctg 41520ctgcaaacgc gcctgctgac ggtctacgag ctgttctagg cggtcctcga tgcgctccac 41580ctggtcatgc tttgcctgca cgtagagcgc aagggtctgc tggtaggtct gctcgatggg 41640cgcggattct aagagggcct gctgttccgt ctcggcctcc tgggccgcct gtagcaaatc 41700ctcgccgctg ttgccgctgg actgctttac tgccggggac tgctgttgcc ctgctcgcgc 41760cgtcgtcgca gttcggcttg cccccactcg attgactgct tcatttcgag ccgcagcgat 41820gcgatctcgg attgcgtcaa cggacggggc agcgcggagg tgtccggctt ctccttgggt 41880gagtcggtcg atgccatagc caaaggtttc cttccaaaat gcgtccattg ctggaccgtg 41940tttctcattg atgcccgcaa gcatcttcgg cttgaccgcc aggtcaagcg cgccttcatg 42000ggcggtcatg acggacgccg ccatgacctt gccgccgttg ttctcgatgt agccgcgtaa 42060tgaggcaatg gtgccgccca tcgtcagcgt gtcatcgaca acgatgtact tctggccggg 42120gatcacctcc ccctcgaaag tcgggttgaa cgccaggcga tgatctgaac cggctccggt 42180tcgggcgacc ttctcccgct gcacaatgtc cgtttcgacc tcaaggccaa ggcggtcggc 42240cagaacgacc gccatcatgg ccggaatctt gttgttcccc gccgcctcga cggcgaggac 42300tggaacgatg cggggcttgt cgtcgccgat cagcgtcttg agctgggcaa cagtgtcgtc 42360cgaaatcagg cgctcgacca aattaagcgc cgcttccgcg tcgccctgct tcgcagcctg 42420gtattcaggc tcgttggtca aagaaccaag gtcgccgttg cgaaccacct tcgggaagtc 42480tccccacggt gcgcgctcgg ctctgctgta gctgctcaag acgcctccct ttttagccgc 42540taaaactcta acgagtgcgc ccgcgactca acttgacgct ttcggcactt acctgtgcct 42600tgccacttgc gtcataggtg atgcttttcg cactcccgat ttcaggtact ttatcgaaat 42660ctgaccgggc gtgcattaca aagttcttcc ccacctgttg gtaaatgctg ccgctatctg 42720cgtggacgat gctgccgtcg tggcgctgcg acttatcggc cttttgggcc atatagatgt 42780tgtaaatgcc aggtttcagg gccccggctt tatctacctt ctggttcgtc catgcgcctt 42840ggttctcggt ctggacaatt ctttgcccat tcatgaccag gaggcggtgt ttcattgggt 42900gactcctgac ggttgcctct ggtgttaaac gtgtcctggt cgcttgccgg ctaaaaaaaa 42960gccgacctcg gcagttcgag gccggctttc cctagagccg ggcgcgtcaa ggttgttcca 43020tctattttag tgaactgcgt tcgatttatc agttactttc ctcccgcttt gtgtttcctc 43080ccactcgttt ccgcgtctag ccgacccctc aacatagcgg cctcttcttg ggctgccttt 43140gcctcttgcc gcgcttcgtc acgctcggct tgcaccgtcg taaagcgctc ggcctgcctg 43200gccgcctctt gcgccgccaa cttcctttgc tcctggtggg cctcggcgtc ggcctgcgcc 43260ttcgctttca ccgctgccaa ctccgtgcgc aaactctccg cttcgcgcct ggtggcgtcg 43320cgctcgccgc gaagcgcctg catttcctgg ttggccgcgt ccagggtctt gcggctctct 43380tctttgaatg cgcgggcgtc ctggtgagcg tagtccagct cggcgcgcag ctcctgcgct 43440cgacgctcca cctcgtcggc ccgctgcgtc gccagcgcgg cccgctgctc ggctcctgcc 43500agggcggtgc gtgcttcggc cagggcttgc cgctggcgtg cggccagctc ggccgcctcg 43560gcggcctgct gctctagcaa tgtaacgcgc gcctgggctt cttccagctc gcgggcctgc 43620gcctcgaagg cgtcggccag ctccccgcgc acggcttcca actcgttgcg ctcacgatcc 43680cagccggctt gcgctgcctg caacgattca ttggcaaggg cctgggcggc ttgccagagg 43740gcggccacgg cctggttgcc ggcctgctgc accgcgtccg gcacctggac tgccagcggg 43800gcggcctgcg ccgtgcgctg gcgtcgccat tcgcgcatgc cggcgctggc gtcgttcatg 43860ttgacgcggg cggccttacg cactgcatcc acggtcggga agttctcccg gtcgccttgc 43920tcgaacagct cgtccgcagc cgcaaaaatg cggtcgcgcg tctctttgtt cagttccatg 43980ttggctccgg taattggtaa gaataataat actcttacct accttatcag cgcaagagtt 44040tagctgaaca gttctcgact taacggcagg ttttttagcg gctgaagggc aggcaaaaaa 44100agccccgcac ggtcggcggg ggcaaagggt cagcgggaag gggattagcg ggcgtcgggc 44160ttcttcatgc gtcggggccg cgcttcttgg gatggagcac gacgaagcgc gcacgcgcat 44220cgtcctcggc cctatcggcc cgcgtcgcgg tcaggaactt gtcgcgcgct aggtcctccc 44280tggtgggcac caggggcatg aactcggcct gctcgatgta ggtccactcc atgaccgcat 44340cgcagtcgag gccgcgttcc ttcaccgtct cttgcaggtc gcggtacgcc cgctcgttga 44400gcggctggta acgggccaat tggtcgtaaa tggctgtcgg ccatgagcgg cctttcctgt 44460tgagccagca gccgacgacg aagccggcaa tgcaggcccc tggcacaacc aggccgacgc 44520cgggggcagg ggatggcagc agctcgccaa ccaggaaccc cgccgcgatg atgccgatgc 44580cggtcaacca gcccttgaaa ctatccggcc ccgaaacacc cctgcgcatt gcctggatgc 44640tgcgccggat agcttgcaac atcaggagcc gtttcttttg ttcgtcagtc atggtccgcc 44700ctcaccagtt gttcgtatcg gtgtcggacg aactgaaatc gcaagagctg ccggtatcgg 44760tccagccgct gtccgtgtcg ctgctgccga agcacggcga ggggtccgcg aacgccgcag 44820acggcgtatc cggccgcagc gcatcgccca gcatggcccc ggtcagcgag ccgccggcca 44880ggtagcccag catggtgctg ttggtcgccc cggccaccag ggccgacgtg acgaaatcgc 44940cgtcattccc tctggattgt tcgctgctcg gcggggcagt gcgccgcgcc ggcggcgtcg 45000tggatggctc gggttggctg gcctgcgacg gccggcgaaa ggtgcgcagc agctcgttat 45060cgaccggctg cggcgtcggg gccgccgcct tgcgctgcgg tcggtgttcc ttcttcggct 45120cgcgcagctt gaacagcatg atcgcggaaa ccagcagcaa cgccgcgcct acgcctcccg 45180cgatgtagaa cagcatcgga ttcattcttc ggtcctcctt gtagcggaac cgttgtctgt 45240gcggcgcggg tggcccgcgc cgctgtcttt ggggatcagc cctcgatgag cgcgaccagt 45300ttcacgtcgg caaggttcgc ctcgaactcc tggccgtcgt cctcgtactt caaccaggca 45360tagccttccg ccggcggccg acggttgagg ataaggcggg cagggcgctc gtcgtgctcg 45420acctggacga tggccttttt cagcttgtcc gggtccggct ccttcgcgcc cttttccttg 45480gcgtccttac cgtcctggtc gccgtcctcg ccgtcctggc cgtcgccggc ctccgcgtca 45540cgctcggcat cagtctggcc gttgaaggca tcgacggtgt tgggatcgcg gcccttctcg 45600tccaggaact cgcgcagcag cttgaccgtg ccgcgcgtga tttcctgggt gtcgtcgtca 45660agccacgcct cgacttcctc cgggcgcttc ttgaaggccg tcaccagctc gttcaccacg 45720gtcacgtcgc gcacgcggcc ggtgttgaac gcatcggcga tcttctccgg caggtccagc 45780agcgtgacgt gctgggtgat gaacgccggc gacttgccga tttccttggc gatatcgcct 45840ttcttcttgc ccttcgccag ctcgcggcca atgaagtcgg caatttcgcg cggggtcagc 45900tcgttgcgtt gcaggttctc gataacctgg tcggcttcgt tgtagtcgtt gtcgatgaac 45960gccgggatgg acttcttgcc ggcccacttc gagccacggt agcggcgggc gccgtgattg 46020atgatatagc ggcccggctg ctcctggttc tcgcgcaccg aaatgggtga cttcaccccg 46080cgctctttga tcgtggcacc gatttccgcg atgctctccg gggaaaagcc ggggttgtcg 46140gccgtccgcg gctgatgcgg atcttcgtcg atcaggtcca ggtccagctc gatagggccg 46200gaaccgccct gagacgccgc aggagcgtcc aggaggctcg acaggtcgcc gatgctatcc 46260aaccccaggc cggacggctg cgccgcgcct gcggcttcct gagcggccgc agcggtgttt 46320ttcttggtgg tcttggcttg agccgcagtc attgggaaat ctccatcttc gtgaacacgt 46380aatcagccag ggcgcgaacc tctttcgatg ccttgcgcgc ggccgttttc ttgatcttcc 46440agaccggcac accggatgcg agggcatcgg cgatgctgct gcgcaggcca acggtggccg 46500gaatcatcat cttggggtac gcggccagca gctcggcttg gtggcgcgcg tggcgcggat 46560tccgcgcatc gaccttgctg ggcaccatgc caaggaattg cagcttggcg ttcttctggc 46620gcacgttcgc aatggtcgtg accatcttct tgatgccctg gatgctgtac gcctcaagct 46680cgatggggga cagcacatag tcggccgcga agagggcggc cgccaggccg acgccaaggg 46740tcggggccgt gtcgatcagg cacacgtcga agccttggtt cgccagggcc ttgatgttcg 46800ccccgaacag ctcgcgggcg tcgtccagcg acagccgttc ggcgttcgcc agtaccgggt 46860tggactcgat gagggcgagg cgcgcggcct ggccgtcgcc ggctgcgggt gcggtttcgg 46920tccagccgcc ggcagggaca gcgccgaaca gcttgcttgc atgcaggccg gtagcaaagt 46980ccttgagcgt gtaggacgca ttgccctggg ggtccaggtc gatcacggca acccgcaagc 47040cgcgctcgaa aaagtcgaag gcaagatgca caagggtcga agtcttgccg acgccgcctt 47100tctggttggc cgtgaccaaa gttttcatcg tttggtttcc tgttttttct tggcgtccgc 47160ttcccacttc cggacgatgt acgcctgatg ttccggcaga accgccgtta cccgcgcgta 47220cccctcgggc aagttcttgt cctcgaacgc ggcccacacg cgatgcaccg cttgcgacac 47280tgcgcccctg gtcagtccca gcgacgttgc gaacgtcgcc tgtggcttcc catcgactaa 47340gacgccccgc gctatctcga tggtctgctg ccccacttcc agcccctgga tcgcctcctg 47400gaactggctt tcggtaagcc gtttcttcat ggataacacc cataatttgc tccgcgcctt 47460ggttgaacat agcggtgaca gccgccagca catgagagaa gtttagctaa acatttctcg 47520cacgtcaaca cctttagccg ctaaaactcg tccttggcgt aacaaaacaa aagcccggaa 47580accgggcttt cgtctcttgc cgcttatggc tctgcacccg gctccatcac caacaggtcg 47640cgcacgcgct tcactcggtt gcggatcgac actgccagcc caacaaagcc ggttgccgcc 47700gccgccagga tcgcgccgat gatgccggcc acaccggcca tcgcccacca ggtcgccgcc 47760ttccggttcc attcctgctg gtactgcttc gcaatgctgg acctcggctc accataggct 47820gaccgctcga tggcgtatgc cgcttctccc cttggcgtaa aacccagcgc cgcaggcggc 47880attgccatgc tgcccgccgc tttcccgacc acgacgcgcg caccaggctt gcggtccaga 47940ccttcggcca cggcgagctg cgcaaggaca taatcagccg ccgacttggc tccacgcgcc 48000tcgatcagct cttgcactcg cgcgaaatcc ttggcctcca cggccgccat gaatcgcgca 48060cgcggcgaag gctccgcagg gccggcgtcg tgatcgccgc cgagaatgcc cttcaccaag 48120ttcgacgaca cgaaaatcat gctgacggct atcaccatca tgcagacgga tcgcacgaac 48180ccgctgaatt gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa 48240aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc 48300cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca 48360cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg 48420ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg 48480cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 48540cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 48600ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 48660ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 48720aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 48780gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 48840aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 48900gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 48960gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 49020aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 49080ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 49140agcccggtga gcgtcggaaa ggcgctggaa gccccgtagc gacgcggaga ggggcgagac 49200aagccaaggg cgcaggctcg atgcgcagca cgacatagcc ggttctcgca aggacgagaa 49260tttccctgcg gtgcccctca agtgtcaatg aaagtttcca acgcgagcca ttcgcgagag 49320ccttgagtcc acgctagatg agagctttgt tgtaggtgga ccagttggtg attttgaact 49380tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 49440cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 49500ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 49560atacaagggg tgttatgagc catattcaac gggaaac 495979449579DNAArtificial SeqeunceVector 94gtcttgctcg actctagagc tcgttcctcg aggcctcgag gcctcgagga acggtacctg 60cggggaagct tacaataatg tgtgttgtta agtcttgttg cctgtcatcg tctgactgac 120tttcgtcata aatcccggcc tccgtaaccc agctttgggc aagctcacgg atttgatccg 180gcggaacggg aatatcgaga tgccgggctg aacgctgcag ttccagcttt ccctttcggg 240acaggtactc cagctgattg attatctgct gaagggtctt ggttccacct cctggcacaa 300tgcgaatgat tacttgagcg cgatcgggca tccaattttc tcccgtcagg tgcgtggtca 360agtgctacaa ggcacctttc agtaacgagc gaccgtcgat ccgtcgccgg gatacggaca 420aaatggagcg cagtagtcca tcgagggcgg cgaaagcctc gccaaaagca atacgttcat 480ctcgcacagc ctccagatcc gatcgagggt cttcggcgta ggcagataga agcatggata 540cattgcttga gagtattccg atggactgaa gtatggcttc catcttttct cgtgtgtctg 600catctatttc gagaaagccc ccgatgcggc gcaccgcaac gcgaattgcc atactatccg 660aaagtcccag caggcgcgct tgataggaaa aggtttcata ctcggccgat cgcagacggg 720cactcacgac cttgaaccct tcaactttca gggatcgatg ctggttgatg gtagtctcac 780tcgacgtggc tctggtgtgt tttgacatag cttcctccaa agaaagcgga aggtctggat 840actccagcac gaaatgtgcc cgggtagacg gatggaagtc tagccctgct caatatgaaa 900tcaacagtac atttacagtc aatactgaat atacttgcta catttgcaat tgtcttataa 960cgaatgtgaa ataaaaatag tgtaacaacg cttttactca tcgataatca caaaaacatt 1020tatacgaaca aaaatacaaa tgcactccgg tttcacagga taggcgggat cagaatatgc 1080aacttttgac gttttgttct ttcaaagggg gtgctggcaa aaccaccgca ctcatgggcc 1140tttgcgctgc tttggcaaat gacggtaaac gagtggccct ctttgatgcc gacgaaaacc 1200ggcctctgac gcgatggaga gaaaacgcct tacaaagcag tactgggatc ctcgctgtga 1260agtctattcc gccgacgaaa tgccccttct tgaagcagcc tatgaaaatg ccgagctcga 1320aggatttgat tatgcgttgg ccgatacgcg tggcggctcg agcgagctca acaacacaat 1380catcgctagc tcaaacctgc ttctgatccc caccatgcta acgccgctcg acatcgatga 1440ggcactatct acctaccgct acgtcatcga gctgctgttg agtgaaaatt tggcaattcc 1500tacagctgtt ttgcgccaac gcgtcccggt cggccgattg acaacatcgc aacgcaggat 1560gtcagagacg ctagagagcc ttccagttgt accgtctccc atgcatgaaa gagatgcatt 1620tgccgcgatg aaagaacgcg gcatgttgca tcttacatta ctaaacacgg gaactgatcc 1680gacgatgcgc ctcatagaga ggaatcttcg gattgcgatg gaggaagtcg tggtcatttc 1740gaaactgatc agcaaaatct tggaggcttg aagatggcaa ttcgcaagcc cgcattgtcg 1800gtcggcgaag cacggcggct tgctggtgct cgacccgaga tccaccatcc caacccgaca 1860cttgttcccc agaagctgga cctccagcac ttgcctgaaa aagccgacga gaaagaccag 1920caacgtgagc ctctcgtcgc cgatcacatt tacagtcccg atcgacaact taagctaact 1980gtggatgccc ttagtccacc tccgtccccg aaaaagctcc aggtttttct ttcagcgcga 2040ccgcccgcgc ctcaagtgtc gaaaacatat gacaacctcg ttcggcaata cagtccctcg 2100aagtcgctac aaatgatttt aaggcgcgcg ttggacgatt tcgaaagcat gctggcagat 2160ggatcatttc gcgtggcccc gaaaagttat ccgatccctt caactacaga aaaatccgtt 2220ctcgttcaga cctcacgcat gttcccggtt gcgttgctcg aggtcgctcg aagtcatttt 2280gatccgttgg ggttggagac cgctcgagct ttcggccaca agctggctac cgccgcgctc 2340gcgtcattct ttgctggaga gaagccatcg agcaattggt gaagagggac ctatcggaac 2400ccctcaccaa atattgagtg taggtttgag gccgctggcc gcgtcctcag tcaccttttg 2460agccagataa ttaagagcca aatgcaattg gctcaggctg ccatcgtccc cccgtgcgaa 2520acctgcacgt ccgcgtcaaa gaaataaccg gcacctcttg ctgtttttat cagttgaggg 2580cttgacggat ccgcctcaag tttgcggcgc agccgcaaaa tgagaacatc tatactcctg 2640tcgtaaacct cctcgtcgcg tactcgactg gcaatgagaa gttgctcgcg cgatagaacg 2700tcgcggggtt tctctaaaaa cgcgaggaga agattgaact cacctgccgt aagtttcacc 2760tcaccgccag cttcggacat caagcgacgt tgcctgagat taagtgtcca gtcagtaaaa 2820caaaaagacc gtcggtcttt ggagcggaca acgttggggc gcacgcgcaa ggcaacccga 2880atgcgtgcaa gaaactctct cgtactaaac ggcttagcga taaaatcact tgctcctagc 2940tcgagtgcaa caactttatc cgtctcctca aggcggtcgc cactgataat tatgattgga 3000atatcagact ttgccgccag atttcgaacg atctcaagcc catcttcacg acctaaattt 3060agatcaacaa ccacgacatc gaccgtcgcg gaagagagta ctctagtgaa ctgggtgctg 3120tcggctaccg cggtcacttt gaaggcgtgg atcgtaaggt attcgataat aagatgccgc 3180atagcgacat cgtcatcgat aagaagaacg tgtttcaacg gctcaccttt caatctaaaa 3240tctgaaccct tgttcacagc gcttgagaaa ttttcacgtg aaggatgtac aatcatctcc 3300agctaaatgg gcagttcgtc agaattgcgg ctgaccgcgg atgacgaaaa tgcgaaccaa 3360gtatttcaat tttatgacaa aagttctcaa tcgttgttac aagtgaaacg cttcgaggtt 3420acagctacta ttgattaagg agatcgccta tggtctcgcc ccggcgtcgt gcgtccgccg 3480cgagccagat ctcgcctact tcataaacgt cctcataggc acggaatgga atgatgacat 3540cgatcgccgt agagagcatg tcaatcagtg tgcgatcttc caagctagca ccttgggcgc 3600tacttttgac aagggaaaac agtttcttga atccttggat tggattcgcg ccgtgtattg 3660ttgaaatcga tcccggatgt cccgagacga cttcactcag ataagcccat gctgcatcgt 3720cgcgcatctc gccaagcaat atccggtccg gccgcatacg cagacttgct tggagcaagt 3780gctcggcgct cacagcaccc agcccagcac cgttcttgga gtagagtagt ctaacatgat 3840tatcgtgtgg aatgacgagt tcgagcgtat cttctatggt gattagcctt tcctgggggg 3900ggatggcgct gatcaaggtc ttgctcattg ttgtcttgcc gcttccggta gggccacata 3960gcaacatcgt cagtcggctg acgacgcatg cgtgcagaaa cgcttccaaa tccccgttgt 4020caaaatgctg aaggatagct tcatcatcct gattttggcg tttccttcgt gtctgccact 4080ggttccacct cgaagcatca taacgggagg agacttcttt aagaccagaa acacgcgagc 4140ttggccgtcg aatggtcaag ctgacggtgc ccgagggaac ggtcggcggc agacagattt 4200gtagtcgttc accaccagga agttcagtgg cgcagagggg gttacgtggt ccgacatcct 4260gctttctcag cgcgcccgct aaaatagcga tatcttcaag atcatcataa gagacgggca 4320aaggcatctt ggtaaaaatg ccggcttggc gcacaaatgc ctctccaggt cgattgatcg 4380caatttcttc agtcttcggg tcatcgagcc attccaaaat cggcttcaga agaaagcgta 4440gttgcggatc cacttccatt tacaatgtat cctatctcta agcggaaatt tgaattcatt 4500aagagcggcg gttcctcccc cgcgtggcgc cgccagtcag gcggagctgg taaacaccaa 4560agaaatcgag gtcccgtgct acgaaaatgg aaacggtgtc accctgattc ttcttcaggg 4620ttggcggtat gttgatggtt gccttaaggg ctgtctcagt tgtctgctca ccgttatttt 4680gaaagctgtt gaagctcatc ccgccacccg agctgccggc gtaggtgcta gctgcctgga 4740aggcgccttg aacaacactc aagagcatag ctccgctaaa acgctgccag aagtggctgt 4800cgaccgagcc cggcaatcct gagcgaccga gttcgtccgc gcttggcgat gttaacgaga 4860tcatcgcatg gtcaggtgtc tcggcgcgat cccacaacac aaaaacgcgc ccatctccct 4920gttgcaagcc acgctgtatt tcgccaacaa cggtggtgcc acgatcaaga agcacgatat 4980tgttcgttgt tccacgaata tcctgaggca agacacactt tacatagcct gccaaatttg 5040tgtcgattgc ggtttgcaag atgcacggaa ttattgtccc ttgcgttacc ataaaatcgg 5100ggtgcggcaa gagcgtggcg ctgctgggct gcagctcggt gggtttcata cgtatcgaca 5160aatcgttctc gccggacact tcgccattcg gcaaggagtt gtcgtcacgc ttgccttctt 5220gtcttcggcc cgtgtcgccc tgaatggcgc gtttgctgac cccttgatcg ccgctgctat 5280atgcaaaaat cggtgtttct tccggccgtg gctcatgccg ctccggttcg cccctcggcg 5340gtagaggagc agcaggctga acagcctctt gaaccgctgg aggatccggc ggcacctcaa 5400tcggagctgg atgaaatggc ttggtgtttg ttgcgatcaa agttgacggc gatgcgttct 5460cattcacctt cttttggcgc ccacctagcc aaatgaggct taatgataac gcgagaacga 5520cacctccgac gatcaatttc tgagaccccg aaagacgccg gcgatgtttg tcggagacca 5580gggatccaga tgcatcaacc tcatgtgccg cttgctgact atcgttattc atcccttcgc 5640ccccttcagg acgcgtttca catcgggcct caccgtgccc gtttgcggcc tttggccaac 5700gggatcgtaa gcggtgttcc agatacatag tactgtgtgg ccatccctca gacgccaacc 5760tcgggaaacc gaagaaatct cgacatcgct ccctttaact gaatagttgg caacagcttc 5820cttgccatca ggattgatgg tgtagatgga gggtatgcgt acattgcccg gaaagtggaa 5880taccgtcgta aatccattgt cgaagacttc gagtggcaac agcgaacgat cgccttgggc 5940gacgtagtgc caattactgt ccgccgcacc aagggctgtg acaggctgat ccaataaatt 6000ctcagctttc cgttgatatt gtgcttccgc gtgtagtctg tccacaacag ccttctgttg 6060tgcctccctt cgccgagccg ccgcatcgtc ggcggggtag gcgaattgga cgctgtaata 6120gagatcgggc tgctctttat cgaggtggga cagagtcttg gaacttatac tgaaaacata 6180acggcgcatc ccggagtcgc ttgcggttag cacgattact ggctgaggcg tgaggacctg 6240gcttgccttg aaaaatagat aatttccccg cggtagggct gctagatctt tgctatttga 6300aacggcaacc gctgtcaccg tttcgttcgt ggcgaatgtt acgaccaaag tagctccaac 6360cgccgtcgag aggcgcacca cttgatcggg attgtaagcc aaataacgca tgcgcggatc 6420tagcttgccc gccattggag tgtcttcagc ctccgcacca gtcgcagcgg caaataaaca 6480tgctaaaatg aaaagtgctt ttctgatcat ggttcgctgt ggcctacgtt tgaaacggta 6540tcttccgatg tctgatagga ggtgacaacc agacctgccg ggttggttag tctcaatctg 6600ccgggcaagc tggtcacctt ttcgtagcga actgtcgcgg tccacgtact caccacaggc 6660attttgccgt caacgacgag ggtcctttta tagcgaattt gctgcgtgct tggagttaca 6720tcatttgaag cgatgtgctc gacctccacc ctgccgcgtt tgccaagaat gacttgaggc 6780gaactgggat tgggatagtt gaagaattgc tggtaatcct ggcgcactgt tggggcactg 6840aagttcgata ccaggtcgta

ggcgtactga gcggtgtcgg catcataact ctcgcgcagg 6900cgaacgtact cccacaatga ggcgttaacg acggcctcct cttgagttgc aggcaatcgc 6960gagacagaca cctcgctgtc aacggtgccg tccggccgta tccatagata tacgggcaca 7020agcctgctca acggcaccat tgtggctata gcgaacgctt gagcaacatt tcccaaaatc 7080gcgatagctg cgacagctgc aatgagtttg gagagacgtc gcgccgattt cgctcgcgcg 7140gtttgaaagg cttctacttc cttatagtgc tcggcaaggc tttcgcgcgc cactagcatg 7200gcatattcag gccccgtcat agcgtccacc cgaattgccg agctgaagat ctgacggagt 7260aggctgccat cgccccacat tcagcgggaa gatcgggcct ttgcagctcg ctaatgtgtc 7320gtttgtctgg cagccgctca aagcgacaac taggcacagc aggcaatact tcatagaatt 7380ctccattgag gcgaattttt gcgcgaccta gcctcgctca acctgagcga agcgacggta 7440caagctgctg gcagattggg ttgcgccgct ccagtaactg cctccaatgt tgccggcgat 7500cgccggcaaa gcgacaatga gcgcatcccc tgtcagaaaa aacatatcga gttcgtaaag 7560accaatgatc ttggccgcgg tcgtaccggc gaaggtgatt acaccaagca taagggtgag 7620cgcagtcgct tcggttagga tgacgatcgt tgccacgagg tttaagagga gaagcaagag 7680accgtaggtg ataagttgcc cgatccactt agctgcgatg tcccgcgtgc gatcaaaaat 7740atatccgacg aggatcagag gcccgatcgc gagaagcact ttcgtgagaa ttccaacggc 7800gtcgtaaact ccgaaggcag accagagcgt gccgtaaagg acccactgtg ccccttggaa 7860agcaaggatg tcctggtcgt tcatcggacc gatttcggat gcgattttct gaaaaacggc 7920ctgggtcacg gcgaacattg tatccaactg tgccggaaca gtctgcagag gcaagccggt 7980tacactaaac tgctgaacaa agtttgggac cgtcttttcg aagatggaaa ccacatagtc 8040ttggtagtta gcctgcccaa caattagagc aacaacgatg gtgaccgtga tcacccgagt 8100gataccgcta cgggtatcga cttcgccgcg tatgactaaa ataccctgaa caataatcca 8160aagagtgaca caggcgatca atggcgcact caccgcctcc tggatagtct caagcatcga 8220gtccaagcct gtcgtgaagg ctacatcgaa gatcgtatga atggccgtaa acggcgccgg 8280aatcgtgaaa ttcatcgatt ggacctgaac ttgactggtt tgtcgcataa tgttggataa 8340aatgagctcg cattcggcga ggatgcgggc ggatgaacaa atcgcccagc cttaggggag 8400ggcaccaaag atgacagcgg tcttttgatg ctccttgcgt tgagcggccg cctcttccgc 8460ctcgtgaagg ccggcctgcg cggtagtcat cgttaatagg cttgtcgcct gtacattttg 8520aatcattgcg tcatggatct gcttgagaag caaaccattg gtcacggttg cctgcatgat 8580attgcgagat cgggaaagct gagcagacgt atcagcattc gccgtcaagc gtttgtccat 8640cgtttccaga ttgtcagccg caatgccagc gctgtttgcg gaaccggtga tctgcgatcg 8700caacaggtcc gcttcagcat cactacccac gactgcacga tctgtatcgc tggtgatcgc 8760acgtgccgtg gtcgacattg gcattcgcgg cgaaaacatt tcattgtcta ggtccttcgt 8820cgaaggatac tgatttttct ggttgagcga agtcagtagt ccagtaacgc cgtaggccga 8880cgtcaacatc gtaaccatcg ctatagtctg agtgagattc tccgcagtcg cgagcgcagt 8940cgcgagcgtc tcagcctccg ttgccgggtc gctaacaaca aactgcgccc gcgcgggctg 9000aatatataga aagctgcagg tcaaaactgt tgcaataagt tgcgtcgtct tcatcgtttc 9060ctaccttatc aatcttctgc ctcgtggtga cgggccatga attcgctgag ccagccagat 9120gagttgcctt cttgtgcctc gcgtagtcga gttgcaaagc gcaccgtgtt ggcacgcccc 9180gaaagcacgg cgacatattc acgcatatcc cgcagatcaa attcgcagat gacgcttcca 9240ctttctcgtt taagaagaaa cttacggctg ccgaccgtca tgtcttcacg gatcgcctga 9300aattcctttt cggtacattt cagtccatcg acataagccg atcgatctgc ggttggtgat 9360ggatagaaaa tcttcgtcat acattgcgca accaagctgg ctcctagcgg cgattccaga 9420acatgctctg gttgctgcgt tgccagtatt agcatcccgt tgttttttcg aacggtcagg 9480aggaatttgt cgacgacagt cgaaaattta gggtttaaca aataggcgcg aaactcatcg 9540cagctcatca caaaacggcg gccgtcgatc atggctccaa tccgatgcag gagatatgct 9600gcagcgggag cgcatacttc ctcgtattcg agaagatgcg tcatgtcgaa gccggtaatc 9660gacggatcta actttacttc gtcaacttcg ccgtcaaatg cccagccaag cgcatggccc 9720cggcaccagc gttggagccg cgctcctgcg ccttcggcgg gcccatgcaa caaaaattca 9780cgtaaccccg cgattgaacg catttgtgga tcaaacgaga gctgacgatg gataccacgg 9840accagacggc ggttctcttc cggagaaatc ccaccccgac catcactctc gatgagagcc 9900acgatccatt cgcgcagaaa atcgtgtgag gctgctgtgt tttctaggcc acgcaacggc 9960gccaacccgc tgggtgtgcc tctgtgaagt gccaaatatg ttcctcctgt ggcgcgaacc 10020agcaattcgc caccccggtc cttgtcaaag aacacgaccg tacctgcacg gtcgaccatg 10080ctctgttcga gcatggctag aacaaacatc atgagcgtcg tcttacccct cccgataggc 10140ccgaatattg ccgtcatgcc aacatcgtgc tcatgcggga tatagtcgaa aggcgttccg 10200ccattggtac gaaatcgggc aatcgcgttg ccccagtggc ctgagctggc gccctctgga 10260aagttttcga aagagacaaa ccctgcgaaa ttgcgtgaag tgattgcgcc agggcgtgtg 10320cgccacttaa aattccccgg caattgggac caataggccg cttccatacc aataccttct 10380tggacaacca cggcacctgc atccgccatt cgtgtccgag cccgcgcgcc cctgtcccca 10440agactattga gatcgtctgc atagacgcaa aggctcaaat gatgtgagcc cataacgaat 10500tcgttgctcg caagtgcgtc ctcagcctcg gataatttgc cgatttgagt cacggcttta 10560tcgccggaac tcagcatctg gctcgatttg aggctaagtt tcgcgtgcgc ttgcgggcga 10620gtcaggaacg aaaaactctg cgtgagaaca agtggaaaat cgagggatag cagcgcgttg 10680agcatgcccg gccgtgtttt tgcagggtat tcgcgaaacg aatagatgga tccaacgtaa 10740ctgtcttttg gcgttctgat ctcgagtcct cgcttgccgc aaatgactct gtcggtataa 10800atcgaagcgc cgagtgagcc gctgacgacc ggaaccggtg tgaaccgacc agtcatgatc 10860aaccgtagcg cttcgccaat ttcggtgaag agcacaccct gcttctcgcg gatgccaaga 10920cgatgcaggc catacgcttt aagagagcca gcgacaacat gccaaagatc ttccatgttc 10980ctgatctggc ccgtgagatc gttttccctt tttccgctta gcttggtgaa cctcctcttt 11040accttcccta aagccgcctg tgggtagaca atcaacgtaa ggaagtgttc attgcggagg 11100agttggccgg agagcacgcg ctgttcaaaa gcttcgttca ggctagcggc gaaaacacta 11160cggaagtgtc gcggcgccga tgatggcacg tcggcatgac gtacgaggtg agcatatatt 11220gacacatgat catcagcgat attgcgcaac agcgtgttga acgcacgaca acgcgcattg 11280cgcatttcag tttcctcaag ctcgaatgca acgccatcaa ttctcgcaat ggtcatgatc 11340gatccgtctt caagaaggac gatatggtcg ctgaggtggc caatataagg gagatagatc 11400tcaccggatc tttcggtcgt tccactcgcg ccgagcatca caccattcct ctccctcgtg 11460ggggaaccct aattggattt gggctaacag tagcgccccc ccaaactgca ctatcaatgc 11520ttcttcccgc ggtccgcaaa aatagcagga cgacgctcgc cgcattgtag tctcgctcca 11580cgatgagccg ggctgcaaac cataacggca cgagaacgac ttcgtagagc gggttctgaa 11640cgataacgat gacaaagccg gcgaacatca tgaataaccc tgccaatgtc agtggcaccc 11700caagaaacaa tgcgggccgt gtggctgcga ggtaaagggt cgattcttcc aaacgatcag 11760ccatcaacta ccgccagtga gcgtttggcc gaggaagctc gccccaaaca tgataacaat 11820gccgccgacg acgccggcaa ccagcccaag cgaagcccgc ccgaacatcc aggagatccc 11880gatagcgaca atgccgagaa cagcgagtga ctggccgaac ggaccaagga taaacgtgca 11940tatattgtta accattgtgg cggggtcagt gccgccaccc gcagattgcg ctgcggcggg 12000tccggatgag gaaatgctcc atgcaattgc accgcacaag cttggggcgc agctcgatat 12060cacgcgcatc atcgcattcg agagcgagag gcgatttaga tgtaaacggt atctctcaaa 12120gcatcgcatc aatgcgcacc tccttagtat aagtcgaata agacttgatt gtcgtctgcg 12180gatttgccgt tgtcctggtg tggcggtggc ggagcgatta aaccgccagc gccatcctcc 12240tgcgagcggc gctgatatga cccccaaaca tcccacgtct cttcggattt tagcgcctcg 12300tgatcgtctt ttggaggctc gattaacgcg ggcaccagcg attgagcagc tgtttcaact 12360tttcgcacgt agccgtttgc aaaaccgccg atgaaattac cggtgttgta agcggagatc 12420gcccgacgaa gcgcaaattg cttctcgtca atcgtttcgc cgcctgcata acgacttttc 12480agcatgtttg cagcggcaga taatgatgtg cacgcctgga gcgcaccgtc aggtgtcaga 12540ccgagcatag aaaaatttcg agagtttatt tgcatgaggc caacatccag cgaatgccgt 12600gcatcgagac ggtgcctgac gacttgggtt gcttggctgt gatcttgcca gtgaagcgtt 12660tcgccggtcg tgttgtcatg aatcgctaaa ggatcaaagc gactctccac cttagctatc 12720gccgcaagcg tagatgtcgc aactgatggg gcacacttgc gagcaacatg gtcaaactca 12780gcagatgaga gtggcgtggc aaggctcgac gaacagaagg agaccatcaa ggcaagagaa 12840agcgaccccg atctcttaag cataccttat ctccttagct cgcaactaac accgcctctc 12900ccgttggaag aagtgcgttg ttttatgttg aagattatcg ggagggtcgg ttactcgaaa 12960attttcaatt gcttctttat gatttcaatt gaagcgagaa acctcgcccg gcgtcttgga 13020acgcaacatg gaccgagaac cgcgcatcca tgactaagca accggatcga cctattcagg 13080ccgcagttgg tcaggtcagg ctcagaacga aaatgctcgg cgaggttacg ctgtctgtaa 13140acccattcga tgaacgggaa gcttccttcc gattgctctt ggcaggaata ttggcccatg 13200cctgcttgcg ctttgcaaat gctcttatcg cgttggtatc atatgccttg tccgccagca 13260gaaacgcact ctaagcgatt atttgtaaaa atgtttcggt catgcggcgg tcatgggctt 13320gacccgctgt cagcgcaaga cggatcggtc aaccgtcggc atcgacaaca gcgtgaatct 13380tggtggtcaa accgccacgg gaacgtccca tacagccatc gtcttgatcc cgctgtttcc 13440cgtcgccgca tgttggtgga cgcggacaca ggaactgtca atcatgacga cattctatcg 13500aaagccttgg aaatcacact cagaatatga tcccagacgt ctgcctcacg ccatcgtaca 13560aagcgattgt agcaggttgt acaggaaccg tatcgatcag gaacgtctgc ccagggcggg 13620cccgtccgga agcgccacaa gatgacattg atcacccgcg tcaacgcgcg gcacgcgacg 13680cggcttattt gggaacaaag gactgaacaa cagtccattc gaaatcggtg acatcaaagc 13740ggggacgggt tatcagtggc ctccaagtca agcctcaatg aatcaaaatc agaccgattt 13800gcaaacctga tttatgagtg tgcggcctaa atgatgaaat cgtccttcta gatcgcctcc 13860gtggtgtagc aacacctcgc agtatcgccg tgctgacctt ggccagggaa ttgactggca 13920agggtgcttt cacatgaccg ctcttttggc cgcgatagat gatttcgttg ctgctttggg 13980cacgtagaag gagagaagtc atatcggaga aattcctcct ggcgcgagag cctgctctat 14040cgcgacggca tcccactgtc gggaacagac cggatcattc acgaggcgaa agtcgtcaac 14100acatgcgtta taggcatctt cccttgaagg atgatcttgt tgctgccaat ctggaggtgc 14160ggcagccgca ggcagatgcg atctcagcgc aacttgcggc aaaacatctc actcacctga 14220aaaccactag cgagtctcgc gatcagacga aggcctttta cttaacgaca caatatccga 14280tgtctgcatc acaggcgtcg ctatcccagt caatactaaa gcggtgcagg aactaaagat 14340tactgatgac ttaggcgtgc cacgaggcct gagacgacgc gcgtagacag ttttttgaaa 14400tcattatcaa agtgatggcc tccgctgaag cctatcacct ctgcgccggt ctgtcggaga 14460gatgggcaag cattattacg gtcttcgcgc ccgtacatgc attggacgat tgcagggtca 14520atggatctga gatcatccag aggattgccg cccttacctt ccgtttcgag ttggagccag 14580cccctaaatg agacgacata gtcgacttga tgtgacaatg ccaagagaga gatttgctta 14640acccgatttt tttgctcaag cgtaagccta ttgaagcttg ccggcatgac gtccgcgccg 14700aaagaatatc ctacaagtaa aacattctgc acaccgaaat gcttggtgta gacatcgatt 14760atgtgaccaa gatccttagc agtttcgctt ggggaccgct ccgaccagaa ataccgaagt 14820gaactgacgc caatgacagg aatcccttcc gtctgcagat aggtaccatc gatagatctg 14880ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 14940ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 15000gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 15060tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 15120gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 15180ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 15240gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 15300ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 15360cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 15420ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 15480accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 15540catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 15600gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 15660tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 15720agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 15780actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 15840gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 15900aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 15960gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 16020aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 16080atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 16140gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 16200atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 16260ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 16320cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 16380agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggggg gggggggggg 16440gggttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag 16500ctcgctttca gcacctgtcg tttcctttct tttcagaggg tattttaaat aaaaacatta 16560agttatgacg aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa 16620cccgcgaggt ccctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct 16680acatatcaca acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg 16740tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca 16800gcgacactga atacggggca acctcatgtc cccccccccc ccccccctgc aggcatcgtg 16860gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 16920gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 16980gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17040cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17100ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17160accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 17220aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 17280aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 17340caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 17400ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 17460gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 17520cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 17580aggccctttc gtcttcaaga attggtcgac gatcttgctg cgttcggata ttttcgtgga 17640gttcccgcca cagacccgga ttgaaggcga gatccagcaa ctcgcgccag atcatcctgt 17700gacggaactt tggcgcgtga tgactggcca ggacgtcggc cgaaagagcg acaagcagat 17760cacgcttttc gacagcgtcg gatttgcgat cgaggatttt tcggcgctgc gctacgtccg 17820cgaccgcgtt gagggatcaa gccacagcag cccactcgac cttctagccg acccagacga 17880gccaagggat ctttttggaa tgctgctccg tcgtcaggct ttccgacgtt tgggtggttg 17940aacagaagtc attatcgtac ggaatgccaa gcactcccga ggggaaccct gtggttggca 18000tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 18060taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 18120ttaaactgaa ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt 18180atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa 18240cgttgaagga gccactcagc aagctggtac gattgtaata cgactcacta tagggcgaat 18300tgagcgctgt ttaaacgctc ttcaactgga agagcggtta cccggaccga agcttgcatg 18360cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct 18420aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct 18480atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata 18540atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg 18600agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt 18660tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag 18720ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt 18780tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat 18840ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa 18900aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg 18960acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag 19020acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg 19080gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca 19140cggcaggcgg cctcctcctc ctctcacggc acggcagcta cgggggattc ctttcccacc 19200gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 19260ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 19320cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc cctctctacc 19380ttctctagat cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt 19440ttgtgttaga tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac 19500ctgtacgtca gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg 19560gatggctcta gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat 19620agggtttggt ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc 19680atcttttcat gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc 19740tagatcggag tagaattctg tttcaaacta cctggtggat ttattaattt tggatctgta 19800tgtgtgtgcc atacatattc atagttacga attgaagatg atggatggaa atatcgatct 19860aggataggta tacatgttga tgcgggtttt actgatgcat atacagagat gctttttgtt 19920cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc attcgttcta gatcggagta 19980gaatactgtt tcaaactacc tggtgtattt attaattttg gaactgtatg tgtgtgtcat 20040acatcttcat agttacgagt ttaagatgga tggaaatatc gatctaggat aggtatacat 20100gttgatgtgg gttttactga tgcatataca tgatggcata tgcagcatct attcatatgc 20160tctaaccttg agtacctatc tattataata aacaagtatg ttttataatt attttgatct 20220tgatatactt ggatgatggc atatgcagca gctatatgtg gattttttta gccctgcctt 20280catacgctat ttatttgctt ggtactgttt cttttgtcga tgctcaccct gttgtttggt 20340gttacttctg caggtcgact ctagaggatc tacaagtttg tacaaaaaag caggctccgc 20400ggccgccccc ttcaccatga cgatggctcg tcctggggcg gctttgccgc tgctgctggt 20460cgtggtcggc gcttgctgcg cgcgcctggc ggcggcagtg cacctctccg cgctcggcag 20520gacactcatc gtcgaggcgt cgccgaaggc cggacaagtc ctgcacgccg gcgaggacac 20580gataaccgtg acatggcacc tcaacgcgtc ggcgtccagc gtcgggtaca aggcgctgga 20640ggtgaccctc tgctacgcgc cggcgagcca ggaggaccgc gggtggcgca aggccaacga 20700cgacttgagc aaggacaagg cgtgccagtt caggatcgcc cggcatgcat acgccggcgg 20760ccaggggacg ctccggtaca gggtcgcccg cgacgtcccc accgcgtcct accacgtgcg 20820cgcctacgcg ctggacgcgt ccggggcgcc ggtgggctac ggccagaccg cgcccgccta 20880ctacttccac gtcgcgggcg tctcgggcgt ccacgcgtcc ctccgggtcg ccgccgccgt 20940gctctccgcg ttctccatcg ccgcgctcgc cttctttgtc gtcgtcgaga agaggaggaa 21000ggacgagtag aagggtgggc gcgccgaccc agctttcttg tacaaagtgg tgttaaccta 21060gacttgtcca tcttctggat tggccaactt aattaatgta tgaaataaaa ggatgcacac 21120atagtgacat gctaatcact ataatgtggg catcaaagtt gtgtgttatg tgtaattact 21180agttatctga ataaaagaga aagagatcat ccatatttct tatcctaaat gaatgtcacg 21240tgtctttata attctttgat gaaccagatg catttcatta accaaatcca tatacatata 21300aatattaatc atatataatt aatatcaatt gggttagcaa aacaaatcta gtctaggtgt 21360gttttgcgaa ttgcggccgc caccgcggtg gagctcgaat tccggtccgg gtcacctttg 21420tccaccaaga tggaactgcg gccgctcatt aattaagtca ggcgcgcctc tagttgaaga 21480cacgttcatg tcttcatcgt aagaagacac tcagtagtct tcggccagaa tggccatctg 21540gattcagcag gcctagaagg ccatttaaat cctgaggatc tggtcttcct aaggacccgg 21600gcggtccgat taaactttaa ttcggaccga agcttgcatg cctgcagtgc agcgtgaccc 21660ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac 21720atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa 21780actttactct acgaataata taatctatag tactacaata atatcagtgt tttagagaat 21840catataaatg aacagttaga catggtctaa aggacaattg agtattttga caacaggact 21900ctacagtttt atctttttag

tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc 21960tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt 22020tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa 22080ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa 22140gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc 22200ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa 22260ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg 22320ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca 22380tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc 22440ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 22500ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 22560tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 22620caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg 22680gtccatgcat ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg 22740tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg 22800ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt 22860ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc 22920ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt 22980ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat 23040tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat 23100attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg 23160ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga 23220tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac 23280tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac 23340gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta 23400ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc 23460tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga 23520tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt 23580gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcaggtc 23640gactttaact tagcctagga tccacacgac accatgtccc ccgagcgccg ccccgtcgag 23700atccgcccgg ccaccgccgc cgacatggcc gccgtgtgcg acatcgtgaa ccactacatc 23760gagacctcca ccgtgaactt ccgcaccgag ccgcagaccc cgcaggagtg gatcgacgac 23820ctggagcgcc tccaggaccg ctacccgtgg ctcgtggccg aggtggaggg cgtggtggcc 23880ggcatcgcct acgccggccc gtggaaggcc cgcaacgcct acgactggac cgtggagtcc 23940accgtgtacg tgtcccaccg ccaccagcgc ctcggcctcg gctccaccct ctacacccac 24000ctcctcaaga gcatggaggc ccagggcttc aagtccgtgg tggccgtgat cggcctcccg 24060aacgacccgt ccgtgcgcct ccacgaggcc ctcggctaca ccgcccgcgg caccctccgc 24120gccgccggct acaagcacgg cggctggcac gacgtcggct tctggcagcg cgacttcgag 24180ctgccggccc cgccgcgccc ggtgcgcccg gtgacgcaga tctgagtcga aacctagact 24240tgtccatctt ctggattggc caacttaatt aatgtatgaa ataaaaggat gcacacatag 24300tgacatgcta atcactataa tgtgggcatc aaagttgtgt gttatgtgta attactagtt 24360atctgaataa aagagaaaga gatcatccat atttcttatc ctaaatgaat gtcacgtgtc 24420tttataattc tttgatgaac cagatgcatt tcattaacca aatccatata catataaata 24480ttaatcatat ataattaata tcaattgggt tagcaaaaca aatctagtct aggtgtgttt 24540tgcgaattgc ggccgccacc gcggtggagc tcgaattcat tccgattaat cgtggcctct 24600tgctcttcag gatgaagagc tatgtttaaa cgtgcaagcg ctactagaca attcagtaca 24660ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat 24720atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac 24780tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc 24840agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga 24900aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc 24960ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct tattcatttc 25020tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata ggaaatcgct 25080ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac gttgacgatc 25140gtcgaccgta ccccgatgaa ttaattcgga cgtacgttct gaacacagct ggatacttac 25200ttgggcgatt gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag 25260gttcgtatga cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt 25320attagagagc aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa 25380gcgaaaattg gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat 25440agacgccgcg cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt 25500tcgttgtccc attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc 25560tatatataag cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt 25620agttgctctc agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt 25680cgtagttgct tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt 25740catccactaa aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga 25800ggcttagaac caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt 25860taagccgcgc cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta 25920ccttggtgat ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg 25980ccaagcgatc ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact 26040gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg 26100ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc 26160agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt 26220caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc 26280ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg 26340caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc 26400acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct 26460ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat 26520caagccttac agtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat 26580ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga 26640cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt 26700ttaactcctg aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt 26760catcctgtgc tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc 26820tagttttata cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat 26880tgcaggcagg tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta 26940gtgcccactt tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt 27000gcgacacaac aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct 27060tcccgacaag ctcttggtcg atgaatgcgc catagcaagc agagtcttca tcagagtcat 27120catccgagat gtaatccttc cggtaggggc tcacacttct ggtagatagt tcaaagcctt 27180ggtcggatag gtgcacatcg aacacttcac gaacaatgaa atggttctca gcatccaatg 27240tttccgccac ctgctcaggg atcaccgaaa tcttcatatg acgcctaacg cctggcacag 27300cggatcgcaa acctggcgcg gcttttggca caaaaggcgt gacaggtttg cgaatccgtt 27360gctgccactt gttaaccctt ttgccagatt tggtaactat aatttatgtt agaggcgaag 27420tcttgggtaa aaactggcct aaaattgctg gggatttcag gaaagtaaac atcaccttcc 27480ggctcgatgt ctattgtaga tatatgtagt gtatctactt gatcggggga tctgctgcct 27540cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 27600agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 27660tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 27720cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 27780ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 27840gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 27900atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 27960caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 28020cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 28080taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 28140ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 28200tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 28260gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 28320ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 28380aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 28440aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 28500agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 28560cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 28620gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 28680atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 28740gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 28800tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 28860gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 28920ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 28980actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 29040ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gggggggggg ggggggggac 29100ttccattgtt cattccacgg acaaaaacag agaaaggaaa cgacagaggc caaaaagcct 29160cgctttcagc acctgtcgtt tcctttcttt tcagagggta ttttaaataa aaacattaag 29220ttatgacgaa gaagaacgga aacgccttaa accggaaaat tttcataaat agcgaaaacc 29280cgcgaggtcg ccgccccgta agccgccccg taacctgtcg gatcaccgga aaggacccgt 29340aaagtgataa tgattatcat ctacatatca caacgtgcgt ggaggccatc aaaccacgtc 29400aaataatcaa ttatgacgca ggtatcgtat taattgatct gcatcaactt aacgtaaaaa 29460caacttcaga caatacaaat cagcgacact gaatacgggg caacctcatg tccccccccc 29520ccccccccct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 29580cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 29640ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 29700tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 29760tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 29820cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 29880tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 29940gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 30000tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 30060atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 30120tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 30180cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 30240ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattcggag cttttgccat 30300tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg 30360aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg 30420atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt 30480ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg 30540atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga 30600cttgacggga cggcggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat 30660cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa 30720ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga 30780tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg 30840ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat 30900gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt 30960tgtctacatg gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg 31020tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg 31080acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct 31140atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg 31200gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc 31260atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc 31320gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca 31380tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc 31440cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc 31500agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc 31560cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg 31620tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca 31680ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg 31740accagcggag gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc 31800tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc 31860tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc 31920tgtatgcgtt ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg 31980ggcggcggcc aatcttgctc gtctcgctgg ccggcgccac tgtcgactac gccatcatgg 32040cgacagcgcc tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg 32100cgactggggc ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc 32160ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg 32220gtgggctgat gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg 32280gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc 32340cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg 32400tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg 32460cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt 32520cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag 32580ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct 32640acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg 32700cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg 32760aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg 32820gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat 32880ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga 32940gcggcgcagg gcaacgagcc gatcgctgat cgtggaaacg ataggcctat gccatgcggg 33000tcaaggcgac ttccggcaag ctatacgcgc cctaggagtg cggttggaac gttggcccag 33060ccagatactc ccgatcacga gcaggacgcc gatgatttga agcgcactca gcgtctgatc 33120caagaacaac catcctagca acacggcggt ccccgggctg agaaagccca gtaaggaaac 33180aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc 33240gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg 33300atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt 33360aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat 33420ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt 33480caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg 33540tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc 33600taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa 33660tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat 33720ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc 33780gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc 33840gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta 33900ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc 33960ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat 34020ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat 34080tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga 34140tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg 34200ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc 34260aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct 34320tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg 34380aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt 34440cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca 34500gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca 34560cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg 34620gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa 34680taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc 34740ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca 34800tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa 34860atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca 34920gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac 34980aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg 35040gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata 35100gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga 35160cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg 35220tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg 35280cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc 35340gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct 35400tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct 35460ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc 35520tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc 35580ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag 35640tcggtgcgct cctgcttgtc gccggcatcg ttgcgccact cttcattaac cgctatatcg 35700aaaattgctt gcggcttgtt agaattgcca tgacgtacct cggtgtcacg ggtaagatta 35760ccgataaact ggaactgatt atggctcata tcgaaagtct ccttgagaaa ggagactcta 35820gtttagctaa acattggttc cgctgtcaag aactttagcg gctaaaattt tgcgggccgc 35880gaccaaaggt gcgaggggcg gcttccgctg tgtacaacca gatatttttc accaacatcc 35940ttcgtctgct cgatgagcgg ggcatgacga aacatgagct gtcggagagg gcaggggttt 36000caatttcgtt tttatcagac ttaaccaacg gtaaggccaa cccctcgttg aaggtgatgg 36060aggccattgc cgacgccctg gaaactcccc tacctcttct cctggagtcc accgaccttg 36120accgcgaggc actcgcggag attgcgggtc atcctttcaa gagcagcgtg ccgcccggat 36180acgaacgcat cagtgtggtt ttgccgtcac ataaggcgtt tatcgtaaag aaatggggcg 36240acgacacccg aaaaaagctg cgtggaaggc tctgacgcca agggttaggg cttgcacttc 36300cttctttagc cgctaaaacg gccccttctc tgcgggccgt cggctcgcgc atcatatcga 36360catcctcaac ggaagccgtg ccgcgaatgg catcgggcgg gtgcgctttg acagttgttt 36420tctatcagaa cccctacgtc gtgcggttcg attagctgtt tgtcttgcag gctaaacact 36480ttcggtatat cgtttgcctg tgcgataatg ttgctaatga tttgttgcgt aggggttact 36540gaaaagtgag cgggaaagaa gagtttcaga ccatcaagga gcgggccaag cgcaagctgg 36600aacgcgacat gggtgcggac ctgttggccg cgctcaacga cccgaaaacc gttgaagtca 36660tgctcaacgc ggacggcaag gtgtggcacg aacgccttgg cgagccgatg cggtacatct 36720gcgacatgcg gcccagccag tcgcaggcga ttatagaaac ggtggccgga ttccacggca 36780aagaggtcac gcggcattcg cccatcctgg aaggcgagtt ccccttggat ggcagccgct 36840ttgccggcca attgccgccg gtcgtggccg cgccaacctt tgcgatccgc aagcgcgcgg 36900tcgccatctt cacgctggaa cagtacgtcg aggcgggcat catgacccgc gagcaatacg 36960aggtcattaa aagcgccgtc

gcggcgcatc gaaacatcct cgtcattggc ggtactggct 37020cgggcaagac cacgctcgtc aacgcgatca tcaatgaaat ggtcgccttc aacccgtctg 37080agcgcgtcgt catcatcgag gacaccggcg aaatccagtg cgccgcagag aacgccgtcc 37140aataccacac cagcatcgac gtctcgatga cgctgctgct caagacaacg ctgcgtatgc 37200gccccgaccg catcctggtc ggtgaggtac gtggccccga agcccttgat ctgttgatgg 37260cctggaacac cgggcatgaa ggaggtgccg ccaccctgca cgcaaacaac cccaaagcgg 37320gcctgagccg gctcgccatg cttatcagca tgcacccgga ttcaccgaaa cccattgagc 37380cgctgattgg cgaggcggtt catgtggtcg tccatatcgc caggacccct agcggccgtc 37440gagtgcaaga aattctcgaa gttcttggtt acgagaacgg ccagtacatc accaaaaccc 37500tgtaaggagt atttccaatg acaacggctg ttccgttccg tctgaccatg aatcgcggca 37560ttttgttcta ccttgccgtg ttcttcgttc tcgctctcgc gttatccgcg catccggcga 37620tggcctcgga aggcaccggc ggcagcttgc catatgagag ctggctgacg aacctgcgca 37680actccgtaac cggcccggtg gccttcgcgc tgtccatcat cggcatcgtc gtcgccggcg 37740gcgtgctgat cttcggcggc gaactcaacg ccttcttccg aaccctgatc ttcctggttc 37800tggtgatggc gctgctggtc ggcgcgcaga acgtgatgag caccttcttc ggtcgtggtg 37860ccgaaatcgc ggccctcggc aacggggcgc tgcaccaggt gcaagtcgcg gcggcggatg 37920ccgtgcgtgc ggtagcggct ggacggctcg cctaatcatg gctctgcgca cgatccccat 37980ccgtcgcgca ggcaaccgag aaaacctgtt catgggtggt gatcgtgaac tggtgatgtt 38040ctcgggcctg atggcgtttg cgctgatttt cagcgcccaa gagctgcggg ccaccgtggt 38100cggtctgatc ctgtggttcg gggcgctcta tgcgttccga atcatggcga aggccgatcc 38160gaagatgcgg ttcgtgtacc tgcgtcaccg ccggtacaag ccgtattacc cggcccgctc 38220gaccccgttc cgcgagaaca ccaatagcca agggaagcaa taccgatgat ccaagcaatt 38280gcgattgcaa tcgcgggcct cggcgcgctt ctgttgttca tcctctttgc ccgcatccgc 38340gcggtcgatg ccgaactgaa actgaaaaag catcgttcca aggacgccgg cctggccgat 38400ctgctcaact acgccgctgt cgtcgatgac ggcgtaatcg tgggcaagaa cggcagcttt 38460atggctgcct ggctgtacaa gggcgatgac aacgcaagca gcaccgacca gcagcgcgaa 38520gtagtgtccg cccgcatcaa ccaggccctc gcgggcctgg gaagtgggtg gatgatccat 38580gtggacgccg tgcggcgtcc tgctccgaac tacgcggagc ggggcctgtc ggcgttccct 38640gaccgtctga cggcagcgat tgaagaagag cgctcggtct tgccttgctc gtcggtgatg 38700tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca 38760atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg 38820gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag 38880ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt 38940cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc 39000atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggccgacag 39060gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta 39120caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc 39180ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac 39240cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc 39300acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg 39360gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac 39420cccgaagcag ggttatgcag cggaaaagcg ctgcttccct gctgttttgt ggaatatcta 39480ccgactggaa acaggcaaat gcaggaaatt actgaactga ggggacaggc gagagacgat 39540gccaaagagc tacaccgacg agctggccga gtgggttgaa tcccgcgcgg ccaagaagcg 39600ccggcgtgat gaggctgcgg ttgcgttcct ggcggtgagg gcggatgtcg aggcggcgtt 39660agcgtccggc tatgcgctcg tcaccatttg ggagcacatg cgggaaacgg ggaaggtcaa 39720gttctcctac gagacgttcc gctcgcacgc caggcggcac atcaaggcca agcccgccga 39780tgtgcccgca ccgcaggcca aggctgcgga acccgcgccg gcacccaaga cgccggagcc 39840acggcggccg aagcaggggg gcaaggctga aaagccggcc cccgctgcgg ccccgaccgg 39900cttcaccttc aacccaacac cggacaaaaa ggatctactg taatggcgaa aattcacatg 39960gttttgcagg gcaagggcgg ggtcggcaag tcggccatcg ccgcgatcat tgcgcagtac 40020aagatggaca aggggcagac acccttgtgc atcgacaccg acccggtgaa cgcgacgttc 40080gagggctaca aggccctgaa cgtccgccgg ctgaacatca tggccggcga cgaaattaac 40140tcgcgcaact tcgacaccct ggtcgagctg attgcgccga ccaaggatga cgtggtgatc 40200gacaacggtg ccagctcgtt cgtgcctctg tcgcattacc tcatcagcaa ccaggtgccg 40260gctctgctgc aagaaatggg gcatgagctg gtcatccata ccgtcgtcac cggcggccag 40320gctctcctgg acacggtgag cggcttcgcc cagctcgcca gccagttccc ggccgaagcg 40380cttttcgtgg tctggctgaa cccgtattgg gggcctatcg agcatgaggg caagagcttt 40440gagcagatga aggcgtacac ggccaacaag gcccgcgtgt cgtccatcat ccagattccg 40500gccctcaagg aagaaaccta cggccgcgat ttcagcgaca tgctgcaaga gcggctgacg 40560ttcgaccagg cgctggccga tgaatcgctc acgatcatga cgcggcaacg cctcaagatc 40620gtgcggcgcg gcctgtttga acagctcgac gcggcggccg tgctatgagc gaccagattg 40680aagagctgat ccgggagatt gcggccaagc acggcatcgc cgtcggccgc gacgacccgg 40740tgctgatcct gcataccatc aacgcccggc tcatggccga cagtgcggcc aagcaagagg 40800aaatccttgc cgcgttcaag gaagagctgg aagggatcgc ccatcgttgg ggcgaggacg 40860ccaaggccaa agcggagcgg atgctgaacg cggccctggc ggccagcaag gacgcaatgg 40920cgaaggtaat gaaggacagc gccgcgcagg cggccgaagc gatccgcagg gaaatcgacg 40980acggccttgg ccgccagctc gcggccaagg tcgcggacgc gcggcgcgtg gcgatgatga 41040acatgatcgc cggcggcatg gtgttgttcg cggccgccct ggtggtgtgg gcctcgttat 41100gaatcgcaga ggcgcagatg aaaaagcccg gcgttgccgg gctttgtttt tgcgttagct 41160gggcttgttt gacaggccca agctctgact gcgcccgcgc tcgcgctcct gggcctgttt 41220cttctcctgc tcctgcttgc gcatcagggc ctggtgccgt cgggctgctt cacgcatcga 41280atcccagtcg ccggccagct cgggatgctc cgcgcgcatc ttgcgcgtcg ccagttcctc 41340gatcttgggc gcgtgaatgc ccatgccttc cttgatttcg cgcaccatgt ccagccgcgt 41400gtgcagggtc tgcaagcggg cttgctgttg ggcctgctgc tgctgccagg cggcctttgt 41460acgcggcagg gacagcaagc cgggggcatt ggactgtagc tgctgcaaac gcgcctgctg 41520acggtctacg agctgttcta ggcggtcctc gatgcgctcc acctggtcat gctttgcctg 41580cacgtagagc gcaagggtct gctggtaggt ctgctcgatg ggcgcggatt ctaagagggc 41640ctgctgttcc gtctcggcct cctgggccgc ctgtagcaaa tcctcgccgc tgttgccgct 41700ggactgcttt actgccgggg actgctgttg ccctgctcgc gccgtcgtcg cagttcggct 41760tgcccccact cgattgactg cttcatttcg agccgcagcg atgcgatctc ggattgcgtc 41820aacggacggg gcagcgcgga ggtgtccggc ttctccttgg gtgagtcggt cgatgccata 41880gccaaaggtt tccttccaaa atgcgtccat tgctggaccg tgtttctcat tgatgcccgc 41940aagcatcttc ggcttgaccg ccaggtcaag cgcgccttca tgggcggtca tgacggacgc 42000cgccatgacc ttgccgccgt tgttctcgat gtagccgcgt aatgaggcaa tggtgccgcc 42060catcgtcagc gtgtcatcga caacgatgta cttctggccg gggatcacct ccccctcgaa 42120agtcgggttg aacgccaggc gatgatctga accggctccg gttcgggcga ccttctcccg 42180ctgcacaatg tccgtttcga cctcaaggcc aaggcggtcg gccagaacga ccgccatcat 42240ggccggaatc ttgttgttcc ccgccgcctc gacggcgagg actggaacga tgcggggctt 42300gtcgtcgccg atcagcgtct tgagctgggc aacagtgtcg tccgaaatca ggcgctcgac 42360caaattaagc gccgcttccg cgtcgccctg cttcgcagcc tggtattcag gctcgttggt 42420caaagaacca aggtcgccgt tgcgaaccac cttcgggaag tctccccacg gtgcgcgctc 42480ggctctgctg tagctgctca agacgcctcc ctttttagcc gctaaaactc taacgagtgc 42540gcccgcgact caacttgacg ctttcggcac ttacctgtgc cttgccactt gcgtcatagg 42600tgatgctttt cgcactcccg atttcaggta ctttatcgaa atctgaccgg gcgtgcatta 42660caaagttctt ccccacctgt tggtaaatgc tgccgctatc tgcgtggacg atgctgccgt 42720cgtggcgctg cgacttatcg gccttttggg ccatatagat gttgtaaatg ccaggtttca 42780gggccccggc tttatctacc ttctggttcg tccatgcgcc ttggttctcg gtctggacaa 42840ttctttgccc attcatgacc aggaggcggt gtttcattgg gtgactcctg acggttgcct 42900ctggtgttaa acgtgtcctg gtcgcttgcc ggctaaaaaa aagccgacct cggcagttcg 42960aggccggctt tccctagagc cgggcgcgtc aaggttgttc catctatttt agtgaactgc 43020gttcgattta tcagttactt tcctcccgct ttgtgtttcc tcccactcgt ttccgcgtct 43080agccgacccc tcaacatagc ggcctcttct tgggctgcct ttgcctcttg ccgcgcttcg 43140tcacgctcgg cttgcaccgt cgtaaagcgc tcggcctgcc tggccgcctc ttgcgccgcc 43200aacttccttt gctcctggtg ggcctcggcg tcggcctgcg ccttcgcttt caccgctgcc 43260aactccgtgc gcaaactctc cgcttcgcgc ctggtggcgt cgcgctcgcc gcgaagcgcc 43320tgcatttcct ggttggccgc gtccagggtc ttgcggctct cttctttgaa tgcgcgggcg 43380tcctggtgag cgtagtccag ctcggcgcgc agctcctgcg ctcgacgctc cacctcgtcg 43440gcccgctgcg tcgccagcgc ggcccgctgc tcggctcctg ccagggcggt gcgtgcttcg 43500gccagggctt gccgctggcg tgcggccagc tcggccgcct cggcggcctg ctgctctagc 43560aatgtaacgc gcgcctgggc ttcttccagc tcgcgggcct gcgcctcgaa ggcgtcggcc 43620agctccccgc gcacggcttc caactcgttg cgctcacgat cccagccggc ttgcgctgcc 43680tgcaacgatt cattggcaag ggcctgggcg gcttgccaga gggcggccac ggcctggttg 43740ccggcctgct gcaccgcgtc cggcacctgg actgccagcg gggcggcctg cgccgtgcgc 43800tggcgtcgcc attcgcgcat gccggcgctg gcgtcgttca tgttgacgcg ggcggcctta 43860cgcactgcat ccacggtcgg gaagttctcc cggtcgcctt gctcgaacag ctcgtccgca 43920gccgcaaaaa tgcggtcgcg cgtctctttg ttcagttcca tgttggctcc ggtaattggt 43980aagaataata atactcttac ctaccttatc agcgcaagag tttagctgaa cagttctcga 44040cttaacggca ggttttttag cggctgaagg gcaggcaaaa aaagccccgc acggtcggcg 44100ggggcaaagg gtcagcggga aggggattag cgggcgtcgg gcttcttcat gcgtcggggc 44160cgcgcttctt gggatggagc acgacgaagc gcgcacgcgc atcgtcctcg gccctatcgg 44220cccgcgtcgc ggtcaggaac ttgtcgcgcg ctaggtcctc cctggtgggc accaggggca 44280tgaactcggc ctgctcgatg taggtccact ccatgaccgc atcgcagtcg aggccgcgtt 44340ccttcaccgt ctcttgcagg tcgcggtacg cccgctcgtt gagcggctgg taacgggcca 44400attggtcgta aatggctgtc ggccatgagc ggcctttcct gttgagccag cagccgacga 44460cgaagccggc aatgcaggcc cctggcacaa ccaggccgac gccgggggca ggggatggca 44520gcagctcgcc aaccaggaac cccgccgcga tgatgccgat gccggtcaac cagcccttga 44580aactatccgg ccccgaaaca cccctgcgca ttgcctggat gctgcgccgg atagcttgca 44640acatcaggag ccgtttcttt tgttcgtcag tcatggtccg ccctcaccag ttgttcgtat 44700cggtgtcgga cgaactgaaa tcgcaagagc tgccggtatc ggtccagccg ctgtccgtgt 44760cgctgctgcc gaagcacggc gaggggtccg cgaacgccgc agacggcgta tccggccgca 44820gcgcatcgcc cagcatggcc ccggtcagcg agccgccggc caggtagccc agcatggtgc 44880tgttggtcgc cccggccacc agggccgacg tgacgaaatc gccgtcattc cctctggatt 44940gttcgctgct cggcggggca gtgcgccgcg ccggcggcgt cgtggatggc tcgggttggc 45000tggcctgcga cggccggcga aaggtgcgca gcagctcgtt atcgaccggc tgcggcgtcg 45060gggccgccgc cttgcgctgc ggtcggtgtt ccttcttcgg ctcgcgcagc ttgaacagca 45120tgatcgcgga aaccagcagc aacgccgcgc ctacgcctcc cgcgatgtag aacagcatcg 45180gattcattct tcggtcctcc ttgtagcgga accgttgtct gtgcggcgcg ggtggcccgc 45240gccgctgtct ttggggatca gccctcgatg agcgcgacca gtttcacgtc ggcaaggttc 45300gcctcgaact cctggccgtc gtcctcgtac ttcaaccagg catagccttc cgccggcggc 45360cgacggttga ggataaggcg ggcagggcgc tcgtcgtgct cgacctggac gatggccttt 45420ttcagcttgt ccgggtccgg ctccttcgcg cccttttcct tggcgtcctt accgtcctgg 45480tcgccgtcct cgccgtcctg gccgtcgccg gcctccgcgt cacgctcggc atcagtctgg 45540ccgttgaagg catcgacggt gttgggatcg cggcccttct cgtccaggaa ctcgcgcagc 45600agcttgaccg tgccgcgcgt gatttcctgg gtgtcgtcgt caagccacgc ctcgacttcc 45660tccgggcgct tcttgaaggc cgtcaccagc tcgttcacca cggtcacgtc gcgcacgcgg 45720ccggtgttga acgcatcggc gatcttctcc ggcaggtcca gcagcgtgac gtgctgggtg 45780atgaacgccg gcgacttgcc gatttccttg gcgatatcgc ctttcttctt gcccttcgcc 45840agctcgcggc caatgaagtc ggcaatttcg cgcggggtca gctcgttgcg ttgcaggttc 45900tcgataacct ggtcggcttc gttgtagtcg ttgtcgatga acgccgggat ggacttcttg 45960ccggcccact tcgagccacg gtagcggcgg gcgccgtgat tgatgatata gcggcccggc 46020tgctcctggt tctcgcgcac cgaaatgggt gacttcaccc cgcgctcttt gatcgtggca 46080ccgatttccg cgatgctctc cggggaaaag ccggggttgt cggccgtccg cggctgatgc 46140ggatcttcgt cgatcaggtc caggtccagc tcgatagggc cggaaccgcc ctgagacgcc 46200gcaggagcgt ccaggaggct cgacaggtcg ccgatgctat ccaaccccag gccggacggc 46260tgcgccgcgc ctgcggcttc ctgagcggcc gcagcggtgt ttttcttggt ggtcttggct 46320tgagccgcag tcattgggaa atctccatct tcgtgaacac gtaatcagcc agggcgcgaa 46380cctctttcga tgccttgcgc gcggccgttt tcttgatctt ccagaccggc acaccggatg 46440cgagggcatc ggcgatgctg ctgcgcaggc caacggtggc cggaatcatc atcttggggt 46500acgcggccag cagctcggct tggtggcgcg cgtggcgcgg attccgcgca tcgaccttgc 46560tgggcaccat gccaaggaat tgcagcttgg cgttcttctg gcgcacgttc gcaatggtcg 46620tgaccatctt cttgatgccc tggatgctgt acgcctcaag ctcgatgggg gacagcacat 46680agtcggccgc gaagagggcg gccgccaggc cgacgccaag ggtcggggcc gtgtcgatca 46740ggcacacgtc gaagccttgg ttcgccaggg ccttgatgtt cgccccgaac agctcgcggg 46800cgtcgtccag cgacagccgt tcggcgttcg ccagtaccgg gttggactcg atgagggcga 46860ggcgcgcggc ctggccgtcg ccggctgcgg gtgcggtttc ggtccagccg ccggcaggga 46920cagcgccgaa cagcttgctt gcatgcaggc cggtagcaaa gtccttgagc gtgtaggacg 46980cattgccctg ggggtccagg tcgatcacgg caacccgcaa gccgcgctcg aaaaagtcga 47040aggcaagatg cacaagggtc gaagtcttgc cgacgccgcc tttctggttg gccgtgacca 47100aagttttcat cgtttggttt cctgtttttt cttggcgtcc gcttcccact tccggacgat 47160gtacgcctga tgttccggca gaaccgccgt tacccgcgcg tacccctcgg gcaagttctt 47220gtcctcgaac gcggcccaca cgcgatgcac cgcttgcgac actgcgcccc tggtcagtcc 47280cagcgacgtt gcgaacgtcg cctgtggctt cccatcgact aagacgcccc gcgctatctc 47340gatggtctgc tgccccactt ccagcccctg gatcgcctcc tggaactggc tttcggtaag 47400ccgtttcttc atggataaca cccataattt gctccgcgcc ttggttgaac atagcggtga 47460cagccgccag cacatgagag aagtttagct aaacatttct cgcacgtcaa cacctttagc 47520cgctaaaact cgtccttggc gtaacaaaac aaaagcccgg aaaccgggct ttcgtctctt 47580gccgcttatg gctctgcacc cggctccatc accaacaggt cgcgcacgcg cttcactcgg 47640ttgcggatcg acactgccag cccaacaaag ccggttgccg ccgccgccag gatcgcgccg 47700atgatgccgg ccacaccggc catcgcccac caggtcgccg ccttccggtt ccattcctgc 47760tggtactgct tcgcaatgct ggacctcggc tcaccatagg ctgaccgctc gatggcgtat 47820gccgcttctc cccttggcgt aaaacccagc gccgcaggcg gcattgccat gctgcccgcc 47880gctttcccga ccacgacgcg cgcaccaggc ttgcggtcca gaccttcggc cacggcgagc 47940tgcgcaagga cataatcagc cgccgacttg gctccacgcg cctcgatcag ctcttgcact 48000cgcgcgaaat ccttggcctc cacggccgcc atgaatcgcg cacgcggcga aggctccgca 48060gggccggcgt cgtgatcgcc gccgagaatg cccttcacca agttcgacga cacgaaaatc 48120atgctgacgg ctatcaccat catgcagacg gatcgcacga acccgctgaa ttgaacacga 48180gcacggcacc cgcgaccact atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca 48240tgaagtccgt gaatgccccg acggccgaag tgaagggcag gccgccaccc aggccgccgc 48300cctcactgcc cggcacctgg tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc 48360ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac tgccccgatc ccggcaatgg 48420caaggactgc cagcgctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc 48480cctgggggga tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc 48540cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata 48600aatattggtt taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa 48660cccttgcaaa tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc 48720cctcatctgt cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta 48780gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc 48840tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac 48900gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc 48960cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat 49020ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt 49080gcagggccat agacggccgc cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga 49140aaggcgctgg aagccccgta gcgacgcgga gaggggcgag acaagccaag ggcgcaggct 49200cgatgcgcag cacgacatag ccggttctcg caaggacgag aatttccctg cggtgcccct 49260caagtgtcaa tgaaagtttc caacgcgagc cattcgcgag agccttgagt ccacgctaga 49320tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac 49380ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta 49440ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata 49500tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga 49560gccatattca acgggaaac 495799549015DNAArtificial SeqeunceVector 95gtcttgctcg actctagagc tcgttcctcg aggcctcgag gcctcgagga acggtacctg 60cggggaagct tacaataatg tgtgttgtta agtcttgttg cctgtcatcg tctgactgac 120tttcgtcata aatcccggcc tccgtaaccc agctttgggc aagctcacgg atttgatccg 180gcggaacggg aatatcgaga tgccgggctg aacgctgcag ttccagcttt ccctttcggg 240acaggtactc cagctgattg attatctgct gaagggtctt ggttccacct cctggcacaa 300tgcgaatgat tacttgagcg cgatcgggca tccaattttc tcccgtcagg tgcgtggtca 360agtgctacaa ggcacctttc agtaacgagc gaccgtcgat ccgtcgccgg gatacggaca 420aaatggagcg cagtagtcca tcgagggcgg cgaaagcctc gccaaaagca atacgttcat 480ctcgcacagc ctccagatcc gatcgagggt cttcggcgta ggcagataga agcatggata 540cattgcttga gagtattccg atggactgaa gtatggcttc catcttttct cgtgtgtctg 600catctatttc gagaaagccc ccgatgcggc gcaccgcaac gcgaattgcc atactatccg 660aaagtcccag caggcgcgct tgataggaaa aggtttcata ctcggccgat cgcagacggg 720cactcacgac cttgaaccct tcaactttca gggatcgatg ctggttgatg gtagtctcac 780tcgacgtggc tctggtgtgt tttgacatag cttcctccaa agaaagcgga aggtctggat 840actccagcac gaaatgtgcc cgggtagacg gatggaagtc tagccctgct caatatgaaa 900tcaacagtac atttacagtc aatactgaat atacttgcta catttgcaat tgtcttataa 960cgaatgtgaa ataaaaatag tgtaacaacg cttttactca tcgataatca caaaaacatt 1020tatacgaaca aaaatacaaa tgcactccgg tttcacagga taggcgggat cagaatatgc 1080aacttttgac gttttgttct ttcaaagggg gtgctggcaa aaccaccgca ctcatgggcc 1140tttgcgctgc tttggcaaat gacggtaaac gagtggccct ctttgatgcc gacgaaaacc 1200ggcctctgac gcgatggaga gaaaacgcct tacaaagcag tactgggatc ctcgctgtga 1260agtctattcc gccgacgaaa tgccccttct tgaagcagcc tatgaaaatg ccgagctcga 1320aggatttgat tatgcgttgg ccgatacgcg tggcggctcg agcgagctca acaacacaat 1380catcgctagc tcaaacctgc ttctgatccc caccatgcta acgccgctcg acatcgatga 1440ggcactatct acctaccgct acgtcatcga gctgctgttg agtgaaaatt tggcaattcc 1500tacagctgtt ttgcgccaac gcgtcccggt cggccgattg acaacatcgc aacgcaggat 1560gtcagagacg ctagagagcc ttccagttgt accgtctccc atgcatgaaa gagatgcatt 1620tgccgcgatg aaagaacgcg gcatgttgca tcttacatta ctaaacacgg gaactgatcc 1680gacgatgcgc ctcatagaga ggaatcttcg gattgcgatg gaggaagtcg tggtcatttc 1740gaaactgatc agcaaaatct tggaggcttg aagatggcaa ttcgcaagcc cgcattgtcg 1800gtcggcgaag cacggcggct tgctggtgct cgacccgaga tccaccatcc caacccgaca 1860cttgttcccc agaagctgga cctccagcac ttgcctgaaa aagccgacga gaaagaccag 1920caacgtgagc ctctcgtcgc cgatcacatt tacagtcccg atcgacaact taagctaact 1980gtggatgccc ttagtccacc tccgtccccg aaaaagctcc aggtttttct ttcagcgcga 2040ccgcccgcgc ctcaagtgtc gaaaacatat gacaacctcg ttcggcaata cagtccctcg 2100aagtcgctac aaatgatttt aaggcgcgcg ttggacgatt tcgaaagcat gctggcagat 2160ggatcatttc gcgtggcccc gaaaagttat ccgatccctt caactacaga aaaatccgtt 2220ctcgttcaga cctcacgcat gttcccggtt gcgttgctcg aggtcgctcg aagtcatttt 2280gatccgttgg ggttggagac cgctcgagct ttcggccaca agctggctac cgccgcgctc 2340gcgtcattct ttgctggaga gaagccatcg agcaattggt gaagagggac

ctatcggaac 2400ccctcaccaa atattgagtg taggtttgag gccgctggcc gcgtcctcag tcaccttttg 2460agccagataa ttaagagcca aatgcaattg gctcaggctg ccatcgtccc cccgtgcgaa 2520acctgcacgt ccgcgtcaaa gaaataaccg gcacctcttg ctgtttttat cagttgaggg 2580cttgacggat ccgcctcaag tttgcggcgc agccgcaaaa tgagaacatc tatactcctg 2640tcgtaaacct cctcgtcgcg tactcgactg gcaatgagaa gttgctcgcg cgatagaacg 2700tcgcggggtt tctctaaaaa cgcgaggaga agattgaact cacctgccgt aagtttcacc 2760tcaccgccag cttcggacat caagcgacgt tgcctgagat taagtgtcca gtcagtaaaa 2820caaaaagacc gtcggtcttt ggagcggaca acgttggggc gcacgcgcaa ggcaacccga 2880atgcgtgcaa gaaactctct cgtactaaac ggcttagcga taaaatcact tgctcctagc 2940tcgagtgcaa caactttatc cgtctcctca aggcggtcgc cactgataat tatgattgga 3000atatcagact ttgccgccag atttcgaacg atctcaagcc catcttcacg acctaaattt 3060agatcaacaa ccacgacatc gaccgtcgcg gaagagagta ctctagtgaa ctgggtgctg 3120tcggctaccg cggtcacttt gaaggcgtgg atcgtaaggt attcgataat aagatgccgc 3180atagcgacat cgtcatcgat aagaagaacg tgtttcaacg gctcaccttt caatctaaaa 3240tctgaaccct tgttcacagc gcttgagaaa ttttcacgtg aaggatgtac aatcatctcc 3300agctaaatgg gcagttcgtc agaattgcgg ctgaccgcgg atgacgaaaa tgcgaaccaa 3360gtatttcaat tttatgacaa aagttctcaa tcgttgttac aagtgaaacg cttcgaggtt 3420acagctacta ttgattaagg agatcgccta tggtctcgcc ccggcgtcgt gcgtccgccg 3480cgagccagat ctcgcctact tcataaacgt cctcataggc acggaatgga atgatgacat 3540cgatcgccgt agagagcatg tcaatcagtg tgcgatcttc caagctagca ccttgggcgc 3600tacttttgac aagggaaaac agtttcttga atccttggat tggattcgcg ccgtgtattg 3660ttgaaatcga tcccggatgt cccgagacga cttcactcag ataagcccat gctgcatcgt 3720cgcgcatctc gccaagcaat atccggtccg gccgcatacg cagacttgct tggagcaagt 3780gctcggcgct cacagcaccc agcccagcac cgttcttgga gtagagtagt ctaacatgat 3840tatcgtgtgg aatgacgagt tcgagcgtat cttctatggt gattagcctt tcctgggggg 3900ggatggcgct gatcaaggtc ttgctcattg ttgtcttgcc gcttccggta gggccacata 3960gcaacatcgt cagtcggctg acgacgcatg cgtgcagaaa cgcttccaaa tccccgttgt 4020caaaatgctg aaggatagct tcatcatcct gattttggcg tttccttcgt gtctgccact 4080ggttccacct cgaagcatca taacgggagg agacttcttt aagaccagaa acacgcgagc 4140ttggccgtcg aatggtcaag ctgacggtgc ccgagggaac ggtcggcggc agacagattt 4200gtagtcgttc accaccagga agttcagtgg cgcagagggg gttacgtggt ccgacatcct 4260gctttctcag cgcgcccgct aaaatagcga tatcttcaag atcatcataa gagacgggca 4320aaggcatctt ggtaaaaatg ccggcttggc gcacaaatgc ctctccaggt cgattgatcg 4380caatttcttc agtcttcggg tcatcgagcc attccaaaat cggcttcaga agaaagcgta 4440gttgcggatc cacttccatt tacaatgtat cctatctcta agcggaaatt tgaattcatt 4500aagagcggcg gttcctcccc cgcgtggcgc cgccagtcag gcggagctgg taaacaccaa 4560agaaatcgag gtcccgtgct acgaaaatgg aaacggtgtc accctgattc ttcttcaggg 4620ttggcggtat gttgatggtt gccttaaggg ctgtctcagt tgtctgctca ccgttatttt 4680gaaagctgtt gaagctcatc ccgccacccg agctgccggc gtaggtgcta gctgcctgga 4740aggcgccttg aacaacactc aagagcatag ctccgctaaa acgctgccag aagtggctgt 4800cgaccgagcc cggcaatcct gagcgaccga gttcgtccgc gcttggcgat gttaacgaga 4860tcatcgcatg gtcaggtgtc tcggcgcgat cccacaacac aaaaacgcgc ccatctccct 4920gttgcaagcc acgctgtatt tcgccaacaa cggtggtgcc acgatcaaga agcacgatat 4980tgttcgttgt tccacgaata tcctgaggca agacacactt tacatagcct gccaaatttg 5040tgtcgattgc ggtttgcaag atgcacggaa ttattgtccc ttgcgttacc ataaaatcgg 5100ggtgcggcaa gagcgtggcg ctgctgggct gcagctcggt gggtttcata cgtatcgaca 5160aatcgttctc gccggacact tcgccattcg gcaaggagtt gtcgtcacgc ttgccttctt 5220gtcttcggcc cgtgtcgccc tgaatggcgc gtttgctgac cccttgatcg ccgctgctat 5280atgcaaaaat cggtgtttct tccggccgtg gctcatgccg ctccggttcg cccctcggcg 5340gtagaggagc agcaggctga acagcctctt gaaccgctgg aggatccggc ggcacctcaa 5400tcggagctgg atgaaatggc ttggtgtttg ttgcgatcaa agttgacggc gatgcgttct 5460cattcacctt cttttggcgc ccacctagcc aaatgaggct taatgataac gcgagaacga 5520cacctccgac gatcaatttc tgagaccccg aaagacgccg gcgatgtttg tcggagacca 5580gggatccaga tgcatcaacc tcatgtgccg cttgctgact atcgttattc atcccttcgc 5640ccccttcagg acgcgtttca catcgggcct caccgtgccc gtttgcggcc tttggccaac 5700gggatcgtaa gcggtgttcc agatacatag tactgtgtgg ccatccctca gacgccaacc 5760tcgggaaacc gaagaaatct cgacatcgct ccctttaact gaatagttgg caacagcttc 5820cttgccatca ggattgatgg tgtagatgga gggtatgcgt acattgcccg gaaagtggaa 5880taccgtcgta aatccattgt cgaagacttc gagtggcaac agcgaacgat cgccttgggc 5940gacgtagtgc caattactgt ccgccgcacc aagggctgtg acaggctgat ccaataaatt 6000ctcagctttc cgttgatatt gtgcttccgc gtgtagtctg tccacaacag ccttctgttg 6060tgcctccctt cgccgagccg ccgcatcgtc ggcggggtag gcgaattgga cgctgtaata 6120gagatcgggc tgctctttat cgaggtggga cagagtcttg gaacttatac tgaaaacata 6180acggcgcatc ccggagtcgc ttgcggttag cacgattact ggctgaggcg tgaggacctg 6240gcttgccttg aaaaatagat aatttccccg cggtagggct gctagatctt tgctatttga 6300aacggcaacc gctgtcaccg tttcgttcgt ggcgaatgtt acgaccaaag tagctccaac 6360cgccgtcgag aggcgcacca cttgatcggg attgtaagcc aaataacgca tgcgcggatc 6420tagcttgccc gccattggag tgtcttcagc ctccgcacca gtcgcagcgg caaataaaca 6480tgctaaaatg aaaagtgctt ttctgatcat ggttcgctgt ggcctacgtt tgaaacggta 6540tcttccgatg tctgatagga ggtgacaacc agacctgccg ggttggttag tctcaatctg 6600ccgggcaagc tggtcacctt ttcgtagcga actgtcgcgg tccacgtact caccacaggc 6660attttgccgt caacgacgag ggtcctttta tagcgaattt gctgcgtgct tggagttaca 6720tcatttgaag cgatgtgctc gacctccacc ctgccgcgtt tgccaagaat gacttgaggc 6780gaactgggat tgggatagtt gaagaattgc tggtaatcct ggcgcactgt tggggcactg 6840aagttcgata ccaggtcgta ggcgtactga gcggtgtcgg catcataact ctcgcgcagg 6900cgaacgtact cccacaatga ggcgttaacg acggcctcct cttgagttgc aggcaatcgc 6960gagacagaca cctcgctgtc aacggtgccg tccggccgta tccatagata tacgggcaca 7020agcctgctca acggcaccat tgtggctata gcgaacgctt gagcaacatt tcccaaaatc 7080gcgatagctg cgacagctgc aatgagtttg gagagacgtc gcgccgattt cgctcgcgcg 7140gtttgaaagg cttctacttc cttatagtgc tcggcaaggc tttcgcgcgc cactagcatg 7200gcatattcag gccccgtcat agcgtccacc cgaattgccg agctgaagat ctgacggagt 7260aggctgccat cgccccacat tcagcgggaa gatcgggcct ttgcagctcg ctaatgtgtc 7320gtttgtctgg cagccgctca aagcgacaac taggcacagc aggcaatact tcatagaatt 7380ctccattgag gcgaattttt gcgcgaccta gcctcgctca acctgagcga agcgacggta 7440caagctgctg gcagattggg ttgcgccgct ccagtaactg cctccaatgt tgccggcgat 7500cgccggcaaa gcgacaatga gcgcatcccc tgtcagaaaa aacatatcga gttcgtaaag 7560accaatgatc ttggccgcgg tcgtaccggc gaaggtgatt acaccaagca taagggtgag 7620cgcagtcgct tcggttagga tgacgatcgt tgccacgagg tttaagagga gaagcaagag 7680accgtaggtg ataagttgcc cgatccactt agctgcgatg tcccgcgtgc gatcaaaaat 7740atatccgacg aggatcagag gcccgatcgc gagaagcact ttcgtgagaa ttccaacggc 7800gtcgtaaact ccgaaggcag accagagcgt gccgtaaagg acccactgtg ccccttggaa 7860agcaaggatg tcctggtcgt tcatcggacc gatttcggat gcgattttct gaaaaacggc 7920ctgggtcacg gcgaacattg tatccaactg tgccggaaca gtctgcagag gcaagccggt 7980tacactaaac tgctgaacaa agtttgggac cgtcttttcg aagatggaaa ccacatagtc 8040ttggtagtta gcctgcccaa caattagagc aacaacgatg gtgaccgtga tcacccgagt 8100gataccgcta cgggtatcga cttcgccgcg tatgactaaa ataccctgaa caataatcca 8160aagagtgaca caggcgatca atggcgcact caccgcctcc tggatagtct caagcatcga 8220gtccaagcct gtcgtgaagg ctacatcgaa gatcgtatga atggccgtaa acggcgccgg 8280aatcgtgaaa ttcatcgatt ggacctgaac ttgactggtt tgtcgcataa tgttggataa 8340aatgagctcg cattcggcga ggatgcgggc ggatgaacaa atcgcccagc cttaggggag 8400ggcaccaaag atgacagcgg tcttttgatg ctccttgcgt tgagcggccg cctcttccgc 8460ctcgtgaagg ccggcctgcg cggtagtcat cgttaatagg cttgtcgcct gtacattttg 8520aatcattgcg tcatggatct gcttgagaag caaaccattg gtcacggttg cctgcatgat 8580attgcgagat cgggaaagct gagcagacgt atcagcattc gccgtcaagc gtttgtccat 8640cgtttccaga ttgtcagccg caatgccagc gctgtttgcg gaaccggtga tctgcgatcg 8700caacaggtcc gcttcagcat cactacccac gactgcacga tctgtatcgc tggtgatcgc 8760acgtgccgtg gtcgacattg gcattcgcgg cgaaaacatt tcattgtcta ggtccttcgt 8820cgaaggatac tgatttttct ggttgagcga agtcagtagt ccagtaacgc cgtaggccga 8880cgtcaacatc gtaaccatcg ctatagtctg agtgagattc tccgcagtcg cgagcgcagt 8940cgcgagcgtc tcagcctccg ttgccgggtc gctaacaaca aactgcgccc gcgcgggctg 9000aatatataga aagctgcagg tcaaaactgt tgcaataagt tgcgtcgtct tcatcgtttc 9060ctaccttatc aatcttctgc ctcgtggtga cgggccatga attcgctgag ccagccagat 9120gagttgcctt cttgtgcctc gcgtagtcga gttgcaaagc gcaccgtgtt ggcacgcccc 9180gaaagcacgg cgacatattc acgcatatcc cgcagatcaa attcgcagat gacgcttcca 9240ctttctcgtt taagaagaaa cttacggctg ccgaccgtca tgtcttcacg gatcgcctga 9300aattcctttt cggtacattt cagtccatcg acataagccg atcgatctgc ggttggtgat 9360ggatagaaaa tcttcgtcat acattgcgca accaagctgg ctcctagcgg cgattccaga 9420acatgctctg gttgctgcgt tgccagtatt agcatcccgt tgttttttcg aacggtcagg 9480aggaatttgt cgacgacagt cgaaaattta gggtttaaca aataggcgcg aaactcatcg 9540cagctcatca caaaacggcg gccgtcgatc atggctccaa tccgatgcag gagatatgct 9600gcagcgggag cgcatacttc ctcgtattcg agaagatgcg tcatgtcgaa gccggtaatc 9660gacggatcta actttacttc gtcaacttcg ccgtcaaatg cccagccaag cgcatggccc 9720cggcaccagc gttggagccg cgctcctgcg ccttcggcgg gcccatgcaa caaaaattca 9780cgtaaccccg cgattgaacg catttgtgga tcaaacgaga gctgacgatg gataccacgg 9840accagacggc ggttctcttc cggagaaatc ccaccccgac catcactctc gatgagagcc 9900acgatccatt cgcgcagaaa atcgtgtgag gctgctgtgt tttctaggcc acgcaacggc 9960gccaacccgc tgggtgtgcc tctgtgaagt gccaaatatg ttcctcctgt ggcgcgaacc 10020agcaattcgc caccccggtc cttgtcaaag aacacgaccg tacctgcacg gtcgaccatg 10080ctctgttcga gcatggctag aacaaacatc atgagcgtcg tcttacccct cccgataggc 10140ccgaatattg ccgtcatgcc aacatcgtgc tcatgcggga tatagtcgaa aggcgttccg 10200ccattggtac gaaatcgggc aatcgcgttg ccccagtggc ctgagctggc gccctctgga 10260aagttttcga aagagacaaa ccctgcgaaa ttgcgtgaag tgattgcgcc agggcgtgtg 10320cgccacttaa aattccccgg caattgggac caataggccg cttccatacc aataccttct 10380tggacaacca cggcacctgc atccgccatt cgtgtccgag cccgcgcgcc cctgtcccca 10440agactattga gatcgtctgc atagacgcaa aggctcaaat gatgtgagcc cataacgaat 10500tcgttgctcg caagtgcgtc ctcagcctcg gataatttgc cgatttgagt cacggcttta 10560tcgccggaac tcagcatctg gctcgatttg aggctaagtt tcgcgtgcgc ttgcgggcga 10620gtcaggaacg aaaaactctg cgtgagaaca agtggaaaat cgagggatag cagcgcgttg 10680agcatgcccg gccgtgtttt tgcagggtat tcgcgaaacg aatagatgga tccaacgtaa 10740ctgtcttttg gcgttctgat ctcgagtcct cgcttgccgc aaatgactct gtcggtataa 10800atcgaagcgc cgagtgagcc gctgacgacc ggaaccggtg tgaaccgacc agtcatgatc 10860aaccgtagcg cttcgccaat ttcggtgaag agcacaccct gcttctcgcg gatgccaaga 10920cgatgcaggc catacgcttt aagagagcca gcgacaacat gccaaagatc ttccatgttc 10980ctgatctggc ccgtgagatc gttttccctt tttccgctta gcttggtgaa cctcctcttt 11040accttcccta aagccgcctg tgggtagaca atcaacgtaa ggaagtgttc attgcggagg 11100agttggccgg agagcacgcg ctgttcaaaa gcttcgttca ggctagcggc gaaaacacta 11160cggaagtgtc gcggcgccga tgatggcacg tcggcatgac gtacgaggtg agcatatatt 11220gacacatgat catcagcgat attgcgcaac agcgtgttga acgcacgaca acgcgcattg 11280cgcatttcag tttcctcaag ctcgaatgca acgccatcaa ttctcgcaat ggtcatgatc 11340gatccgtctt caagaaggac gatatggtcg ctgaggtggc caatataagg gagatagatc 11400tcaccggatc tttcggtcgt tccactcgcg ccgagcatca caccattcct ctccctcgtg 11460ggggaaccct aattggattt gggctaacag tagcgccccc ccaaactgca ctatcaatgc 11520ttcttcccgc ggtccgcaaa aatagcagga cgacgctcgc cgcattgtag tctcgctcca 11580cgatgagccg ggctgcaaac cataacggca cgagaacgac ttcgtagagc gggttctgaa 11640cgataacgat gacaaagccg gcgaacatca tgaataaccc tgccaatgtc agtggcaccc 11700caagaaacaa tgcgggccgt gtggctgcga ggtaaagggt cgattcttcc aaacgatcag 11760ccatcaacta ccgccagtga gcgtttggcc gaggaagctc gccccaaaca tgataacaat 11820gccgccgacg acgccggcaa ccagcccaag cgaagcccgc ccgaacatcc aggagatccc 11880gatagcgaca atgccgagaa cagcgagtga ctggccgaac ggaccaagga taaacgtgca 11940tatattgtta accattgtgg cggggtcagt gccgccaccc gcagattgcg ctgcggcggg 12000tccggatgag gaaatgctcc atgcaattgc accgcacaag cttggggcgc agctcgatat 12060cacgcgcatc atcgcattcg agagcgagag gcgatttaga tgtaaacggt atctctcaaa 12120gcatcgcatc aatgcgcacc tccttagtat aagtcgaata agacttgatt gtcgtctgcg 12180gatttgccgt tgtcctggtg tggcggtggc ggagcgatta aaccgccagc gccatcctcc 12240tgcgagcggc gctgatatga cccccaaaca tcccacgtct cttcggattt tagcgcctcg 12300tgatcgtctt ttggaggctc gattaacgcg ggcaccagcg attgagcagc tgtttcaact 12360tttcgcacgt agccgtttgc aaaaccgccg atgaaattac cggtgttgta agcggagatc 12420gcccgacgaa gcgcaaattg cttctcgtca atcgtttcgc cgcctgcata acgacttttc 12480agcatgtttg cagcggcaga taatgatgtg cacgcctgga gcgcaccgtc aggtgtcaga 12540ccgagcatag aaaaatttcg agagtttatt tgcatgaggc caacatccag cgaatgccgt 12600gcatcgagac ggtgcctgac gacttgggtt gcttggctgt gatcttgcca gtgaagcgtt 12660tcgccggtcg tgttgtcatg aatcgctaaa ggatcaaagc gactctccac cttagctatc 12720gccgcaagcg tagatgtcgc aactgatggg gcacacttgc gagcaacatg gtcaaactca 12780gcagatgaga gtggcgtggc aaggctcgac gaacagaagg agaccatcaa ggcaagagaa 12840agcgaccccg atctcttaag cataccttat ctccttagct cgcaactaac accgcctctc 12900ccgttggaag aagtgcgttg ttttatgttg aagattatcg ggagggtcgg ttactcgaaa 12960attttcaatt gcttctttat gatttcaatt gaagcgagaa acctcgcccg gcgtcttgga 13020acgcaacatg gaccgagaac cgcgcatcca tgactaagca accggatcga cctattcagg 13080ccgcagttgg tcaggtcagg ctcagaacga aaatgctcgg cgaggttacg ctgtctgtaa 13140acccattcga tgaacgggaa gcttccttcc gattgctctt ggcaggaata ttggcccatg 13200cctgcttgcg ctttgcaaat gctcttatcg cgttggtatc atatgccttg tccgccagca 13260gaaacgcact ctaagcgatt atttgtaaaa atgtttcggt catgcggcgg tcatgggctt 13320gacccgctgt cagcgcaaga cggatcggtc aaccgtcggc atcgacaaca gcgtgaatct 13380tggtggtcaa accgccacgg gaacgtccca tacagccatc gtcttgatcc cgctgtttcc 13440cgtcgccgca tgttggtgga cgcggacaca ggaactgtca atcatgacga cattctatcg 13500aaagccttgg aaatcacact cagaatatga tcccagacgt ctgcctcacg ccatcgtaca 13560aagcgattgt agcaggttgt acaggaaccg tatcgatcag gaacgtctgc ccagggcggg 13620cccgtccgga agcgccacaa gatgacattg atcacccgcg tcaacgcgcg gcacgcgacg 13680cggcttattt gggaacaaag gactgaacaa cagtccattc gaaatcggtg acatcaaagc 13740ggggacgggt tatcagtggc ctccaagtca agcctcaatg aatcaaaatc agaccgattt 13800gcaaacctga tttatgagtg tgcggcctaa atgatgaaat cgtccttcta gatcgcctcc 13860gtggtgtagc aacacctcgc agtatcgccg tgctgacctt ggccagggaa ttgactggca 13920agggtgcttt cacatgaccg ctcttttggc cgcgatagat gatttcgttg ctgctttggg 13980cacgtagaag gagagaagtc atatcggaga aattcctcct ggcgcgagag cctgctctat 14040cgcgacggca tcccactgtc gggaacagac cggatcattc acgaggcgaa agtcgtcaac 14100acatgcgtta taggcatctt cccttgaagg atgatcttgt tgctgccaat ctggaggtgc 14160ggcagccgca ggcagatgcg atctcagcgc aacttgcggc aaaacatctc actcacctga 14220aaaccactag cgagtctcgc gatcagacga aggcctttta cttaacgaca caatatccga 14280tgtctgcatc acaggcgtcg ctatcccagt caatactaaa gcggtgcagg aactaaagat 14340tactgatgac ttaggcgtgc cacgaggcct gagacgacgc gcgtagacag ttttttgaaa 14400tcattatcaa agtgatggcc tccgctgaag cctatcacct ctgcgccggt ctgtcggaga 14460gatgggcaag cattattacg gtcttcgcgc ccgtacatgc attggacgat tgcagggtca 14520atggatctga gatcatccag aggattgccg cccttacctt ccgtttcgag ttggagccag 14580cccctaaatg agacgacata gtcgacttga tgtgacaatg ccaagagaga gatttgctta 14640acccgatttt tttgctcaag cgtaagccta ttgaagcttg ccggcatgac gtccgcgccg 14700aaagaatatc ctacaagtaa aacattctgc acaccgaaat gcttggtgta gacatcgatt 14760atgtgaccaa gatccttagc agtttcgctt ggggaccgct ccgaccagaa ataccgaagt 14820gaactgacgc caatgacagg aatcccttcc gtctgcagat aggtaccatc gatagatctg 14880ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 14940ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 15000gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 15060tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 15120gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 15180ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 15240gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 15300ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 15360cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 15420ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 15480accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 15540catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 15600gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 15660tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 15720agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 15780actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 15840gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 15900aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 15960gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 16020aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 16080atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 16140gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 16200atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 16260ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 16320cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 16380agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggggg gggggggggg 16440gggttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag 16500ctcgctttca gcacctgtcg tttcctttct tttcagaggg tattttaaat aaaaacatta 16560agttatgacg aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa 16620cccgcgaggt ccctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct 16680acatatcaca acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg 16740tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca 16800gcgacactga atacggggca acctcatgtc cccccccccc ccccccctgc aggcatcgtg 16860gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 16920gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 16980gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17040cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17100ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17160accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 17220aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 17280aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 17340caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 17400ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg

atacatattt 17460gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 17520cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 17580aggccctttc gtcttcaaga attggtcgac gatcttgctg cgttcggata ttttcgtgga 17640gttcccgcca cagacccgga ttgaaggcga gatccagcaa ctcgcgccag atcatcctgt 17700gacggaactt tggcgcgtga tgactggcca ggacgtcggc cgaaagagcg acaagcagat 17760cacgcttttc gacagcgtcg gatttgcgat cgaggatttt tcggcgctgc gctacgtccg 17820cgaccgcgtt gagggatcaa gccacagcag cccactcgac cttctagccg acccagacga 17880gccaagggat ctttttggaa tgctgctccg tcgtcaggct ttccgacgtt tgggtggttg 17940aacagaagtc attatcgtac ggaatgccaa gcactcccga ggggaaccct gtggttggca 18000tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 18060taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 18120ttaaactgaa ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt 18180atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa 18240cgttgaagga gccactcagc aagctggtac gattgtaata cgactcacta tagggcgaat 18300tgagcgctgt ttaaacgctc ttcaactgga agagcggtta ccagagctgg tcacctttgt 18360ccaccaagat ggaactgcgg ccgctcatta attaagtcag gcgcgcctct agttgaagac 18420acgttcatgt cttcatcgta agaagacact cagtagtctt cggccagaat ggccatctgg 18480attcagcagg cctagaaggc catttaaatc ctgaggatct ggtcttccta aggacccggg 18540atatcgctat caactttgta tagaaaagtt gggccgaatt cgcccttgtt taaacttaat 18600atttgtttaa actttttact aaattcatgt aataattaat gtatgcgtta tatatatatg 18660tctaggttta taattattca tatgaatatg aacataaaaa tctagggcta aaacgactac 18720tattttgaaa acggaaggag tagtaagtta tttaagcgga ggggaaccat gatgggctag 18780tgatttaatt tacatatata tattggtgtt ctgggctctt acatgagaag atctagttaa 18840ctgttgttac tgaacagcga agacaaatat ataatttaag ctccccaact gctagtgatt 18900ctgttaagag gtaatgttta aagtaaattt acaagagccc gtctagctca gtcggtagag 18960cgcaaggctc ttaaccttgt ggtcgtgggt tcgagcccca cggtgggcgc acaatttttt 19020gttttttgac attttttgtt tgcttagttg cagacggttt ttcccctgct aggagatttc 19080cgagagaaaa aaaaggcact acaggttaac caaaaccacc aacctttgga gcgtcgaggc 19140gacggggcat ttgcgtagtt gaagcttaca aagttgcata tgagatgagt gccggacatg 19200aagcggataa cgttttaaac tggcaacaat atctagctgt ttcaaattca ggcgtgggaa 19260gctacgccta cgcgccctgg acggcgtgta aagagccagc atcggcatca ttgtcaaacg 19320atcgacaagg ccaagaaatt ccaaatatat tattaataaa aaagaaggca ccaaattagt 19380ttttgttttt tagtatgtgt ggcggaggaa attttgagaa cgaacgtatc caaagaaggc 19440acaagacgat atagattgac gcggctagaa agttgcagca agacagtggg tacggtctta 19500tatatcctaa taaataaaaa ataaaactat agtgtgtcaa atgtcaacaa gaggaggagg 19560cagccaaatt agcagaggga gacaagtaga gcacgcctta ttagcttgct tatttatcgt 19620ggtggtgtac ttgttaatta ctggcacgca ttatcaacaa cgcagttctg gatgtgaatc 19680tagacaaaca tttgtctagg ttccgcacgt atagtttttt ttcttttttt ttgggggggg 19740gggggaacgg aagctgtaat aaacggtact aggaacgaaa gcaaccgccg cgcgcatgtt 19800tttgcaatag attacggtga ccttgatgca ccaccgcgtg ctataaaaac cagtgtcccc 19860gagtctactc atcaaccaat ccataactcg aaaccttttc ttgtgctctg ttctgtctgt 19920gtgtttccaa agcaagcgaa agaggtcgag gggatcagct tcaagtttgt acaaaaaagc 19980aggctccgcg gccgccccct tcaccatggc tcggcagcaa agcgtgcagg ccttgtgtgt 20040gctggcggcg cttctcttcg ccgcctccct gccgtcgccg gccgccgcgg gggtgcacct 20100ctcctcgctg cccaaagcgc tcgacgtcac cacctccgcc aaacccggcc aagtcctgca 20160cgccggcgtg gactcgctga cggtgacgtg gagcctgaac gccacggagc cggccggcgc 20220cgacgccggg tacaagggcg tgaaggtgaa gctgtgctac gcgccggcga gccagaagga 20280ccgcgggtgg cgcaagtccg aggacgacat cagcaaggac aaggcgtgcc agttcaaggt 20340caccgagcag gcgtacgcgg cggcggcgcc cggcagcttc cagtacgccg tcgcccgcga 20400cgtcccctcg ggctcctact acctgcgcgc cttcgccacg gacgcgtcgg gcgccgaggt 20460ggcctacggc cagacggcgc ccaccgccgc cttcgacgtc gccggcatca ccggcatcca 20520cgcctctctc aagatcgccg ccggcgtctt ctcggccttc tccgtcgtcg cgctcgcctt 20580cttcttcgtc atcgagaccc gcaagaagaa caagtagaag ggtgggcgcg ccgacccagc 20640tttcttgtac aaagtggccg ttaacggatc cagacttgtc catcttctgg attggccaac 20700ttaattaatg tatgaaataa aaggatgcac acatagtgac atgctaatca ctataatgtg 20760ggcatcaaag ttgtgtgtta tgtgtaatta ctagttatct gaataaaaga gaaagagatc 20820atccatattt cttatcctaa atgaatgtca cgtgtcttta taattctttg atgaaccaga 20880tgcatttcat taaccaaatc catatacata taaatattaa tcatatataa ttaatatcaa 20940ttgggttagc aaaacaaatc tagtctaggt gtgttttgcg aattgcggca agcttgcggc 21000cgccccgggc aactttatta tacaaagttg atagatatcg gaccgattaa actttaattc 21060ggtccgaagc ttgcatgcct gcagtgcagc gtgacccggt cgtgcccctc tctagagata 21120atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt 21180tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg aataatataa 21240tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat 21300ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt 21360gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta 21420ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca 21480tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt 21540tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac 21600cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag 21660cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt 21720cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga 21780gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg 21840gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg 21900ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac 21960accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc 22020agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc 22080ccccccccct ctctaccttc tctagatcgg cgttccggtc catgcatggt tagggcccgg 22140tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc cgtgctgcta 22200gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa cttgccagtg 22260tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat cgatttcatg 22320attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt caatatatgc 22380cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt gtgatgatgt 22440ggtctggttg ggcggtcgtt ctagatcgga gtagaattct gtttcaaact acctggtgga 22500tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat 22560gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca 22620tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt 22680cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt 22740ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat 22800cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat 22860atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat 22920gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt 22980ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg 23040atgctcaccc tgttgtttgg tgttacttct gcaggtcgac tttaacttag cctaggatcc 23100acacgacacc atgtcccccg agcgccgccc cgtcgagatc cgcccggcca ccgccgccga 23160catggccgcc gtgtgcgaca tcgtgaacca ctacatcgag acctccaccg tgaacttccg 23220caccgagccg cagaccccgc aggagtggat cgacgacctg gagcgcctcc aggaccgcta 23280cccgtggctc gtggccgagg tggagggcgt ggtggccggc atcgcctacg ccggcccgtg 23340gaaggcccgc aacgcctacg actggaccgt ggagtccacc gtgtacgtgt cccaccgcca 23400ccagcgcctc ggcctcggct ccaccctcta cacccacctc ctcaagagca tggaggccca 23460gggcttcaag tccgtggtgg ccgtgatcgg cctcccgaac gacccgtccg tgcgcctcca 23520cgaggccctc ggctacaccg cccgcggcac cctccgcgcc gccggctaca agcacggcgg 23580ctggcacgac gtcggcttct ggcagcgcga cttcgagctg ccggccccgc cgcgcccggt 23640gcgcccggtg acgcagatct gagtcgaaac ctagacttgt ccatcttctg gattggccaa 23700cttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc actataatgt 23760gggcatcaaa gttgtgtgtt atgtgtaatt actagttatc tgaataaaag agaaagagat 23820catccatatt tcttatccta aatgaatgtc acgtgtcttt ataattcttt gatgaaccag 23880atgcatttca ttaaccaaat ccatatacat ataaatatta atcatatata attaatatca 23940attgggttag caaaacaaat ctagtctagg tgtgttttgc gaatgcggcc gccaccgcgg 24000tggagctcga attcattccg attaatcgtg gcctcttgct cttcaggatg aagagctatg 24060tttaaacgtg caagcgctac tagacaattc agtacattaa aaacgtccgc aatgtgttat 24120taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac 24180agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc 24240gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc 24300tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt 24360agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc 24420gcagagatcc gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg 24480atcttgagaa ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga 24540gtggcgctat ttctttagaa gtgaacgttg acgatcgtcg accgtacccc gatgaattaa 24600ttcggacgta cgttctgaac acagctggat acttacttgg gcgattgtca tacatgacat 24660caacaatgta cccgtttgtg taaccgtctc ttggaggttc gtatgacact agtggttccc 24720ctcagcttgc gactagatgt tgaggcctaa cattttatta gagagcaggc tagttgctta 24780gatacatgat cttcaggccg ttatctgtca gggcaagcga aaattggcca tttatgacga 24840ccaatgcccc gcagaagctc ccatctttgc cgccatagac gccgcgcccc ccttttgggg 24900tgtagaacat ccttttgcca gatgtggaaa agaagttcgt tgtcccattg ttggcaatga 24960cgtagtagcc ggcgaaagtg cgagacccat ttgcgctata tataagccta cgatttccgt 25020tgcgactatt gtcgtaattg gatgaactat tatcgtagtt gctctcagag ttgtcgtaat 25080ttgatggact attgtcgtaa ttgcttatgg agttgtcgta gttgcttgga gaaatgtcgt 25140agttggatgg ggagtagtca tagggaagac gagcttcatc cactaaaaca attggcaggt 25200cagcaagtgc ctgccccgat gccatcgcaa gtacgaggct tagaaccacc ttcaacagat 25260cgcgcatagt cttccccagc tctctaacgc ttgagttaag ccgcgccgcg aagcggcgtc 25320ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt 25380agtgaacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat 25440aagcctgcct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc 25500agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg 25560acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg 25620ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct 25680ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca 25740gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt 25800ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa 25860caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca 25920aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacagtc accgtaacca 25980gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta 26040cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag 26100tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctcctgaatt aagccgcgcc 26160gcgaagcggt gtcggcttga atgaattgtt aggcgtcatc ctgtgctccc gagaaccagt 26220accagtacat cgctgtttcg ttcgagactt gaggtctagt tttatacgtg aacaggtcaa 26280tgccgccgag agtaaagcca cattttgcgt acaaattgca ggcaggtaca ttgttcgttt 26340gtgtctctaa tcgtatgcca aggagctgtc tgcttagtgc ccactttttc gcaaattcga 26400tgagactgtg cgcgactcct ttgcctcggt gcgtgtgcga cacaacaatg tgttcgatag 26460aggctagatc gttccatgtt gagttgagtt caatcttccc gacaagctct tggtcgatga 26520atgcgccata gcaagcagag tcttcatcag agtcatcatc cgagatgtaa tccttccggt 26580aggggctcac acttctggta gatagttcaa agccttggtc ggataggtgc acatcgaaca 26640cttcacgaac aatgaaatgg ttctcagcat ccaatgtttc cgccacctgc tcagggatca 26700ccgaaatctt catatgacgc ctaacgcctg gcacagcgga tcgcaaacct ggcgcggctt 26760ttggcacaaa aggcgtgaca ggtttgcgaa tccgttgctg ccacttgtta acccttttgc 26820cagatttggt aactataatt tatgttagag gcgaagtctt gggtaaaaac tggcctaaaa 26880ttgctgggga tttcaggaaa gtaaacatca ccttccggct cgatgtctat tgtagatata 26940tgtagtgtat ctacttgatc gggggatctg ctgcctcgcg cgtttcggtg atgacggtga 27000aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 27060gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat 27120gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag 27180attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 27240taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 27300ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 27360gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 27420gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 27480cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 27540ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 27600tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 27660gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 27720tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 27780ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 27840ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 27900ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 27960accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 28020tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 28080cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 28140taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 28200caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 28260gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 28320gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 28380ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 28440attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 28500gttgccattg ctgcaggggg gggggggggg ggggacttcc attgttcatt ccacggacaa 28560aaacagagaa aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct 28620ttcttttcag agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg 28680ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaagcc 28740gccccgtaac ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac 28800atatcacaac gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta 28860tcgtattaat tgatctgcat caacttaacg taaaaacaac ttcagacaat acaaatcagc 28920gacactgaat acggggcaac ctcatgtccc cccccccccc ccccctgcag gcatcgtggt 28980gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 29040tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 29100cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 29160tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 29220ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac 29280cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 29340actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 29400ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 29460aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 29520ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 29580atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 29640tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 29700gccctttcgt cttcaagaat tcggagcttt tgccattctc accggattca gtcgtcactc 29760atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 29820atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 29880tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 29940ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 30000ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg 30060aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga 30120ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct 30180catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga 30240gtcagcaaca ccttcttcac gaggcagacc tcagcgccag aaggccgcca gagaggccga 30300gcgcggccgt gaggcttgga cgctagggca gggcatgaaa aagcccgtag cgggctgcta 30360cgggcgtctg acgcggtgga aagggggagg ggatgttgtc tacatggctc tgctgtagtg 30420agtgggttgc gctccggcag cggtcctgat caatcgtcac cctttctcgg tccttcaacg 30480ttcctgacaa cgagcctcct tttcgccaat ccatcgacaa tcaccgcgag tccctgctcg 30540aacgctgcgt ccggaccggc ttcgtcgaag gcgtctatcg cggcccgcaa cagcggcgag 30600agcggagcct gttcaacggt gccgccgcgc tcgccggcat cgctgtcgcc ggcctgctcc 30660tcaagcacgg ccccaacagt gaagtagctg attgtcatca gcgcattgac ggcgtccccg 30720gccgaaaaac ccgcctcgca gaggaagcga agctgcgcgt cggccgtttc catctgcggt 30780gcgcccggtc gcgtgccggc atggatgcgc gcgccatcgc ggtaggcgag cagcgcctgc 30840ctgaagctgc gggcattccc gatcagaaat gagcgccagt cgtcgtcggc tctcggcacc 30900gaatgcgtat gattctccgc cagcatggct tcggccagtg cgtcgagcag cgcccgcttg 30960ttcctgaagt gccagtaaag cgccggctgc tgaaccccca accgttccgc cagtttgcgt 31020gtcgtcagac cgtctacgcc gacctcgttc aacaggtcca gggcggcacg gatcactgta 31080ttcggctgca actttgtcat gcttgacact ttatcactga taaacataat atgtccacca 31140acttatcagt gataaagaat ccgcgcgttc aatcggacca gcggaggctg gtccggaggc 31200cagacgtgaa acccaacata cccctgatcg taattctgag cactgtcgcg ctcgacgctg 31260tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg gttcactcga 31320acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttggtg caatttgcct 31380gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc ttgctcgtct 31440cgctggccgg cgccactgtc gactacgcca tcatggcgac agcgcctttc ctttgggttc 31500tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta gccggcgctt 31560atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc atgagcgcct 31620gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc ggtttctccc 31680cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg acgggctgtt 31740tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag gctctcaacc 31800cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg atggcggtct 31860tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt ttcggcgagg 31920atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt ggcattctgc 31980attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc gaaaggcggg 32040cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc ttcgcgacac 32100ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc ggaatgccgg 32160cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag ctgcaaggct 32220cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc acggcgatct 32280atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct gccctctact 32340tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa cgagccgatc 32400gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa ggcgacttcc ggcaagctat 32460acgcgcccta ggagtgcggt tggaacgttg gcccagccag atactcccga

tcacgagcag 32520gacgccgatg atttgaagcg cactcagcgt ctgatccaag aacaaccatc ctagcaacac 32580ggcggtcccc gggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat 32640cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc 32700gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac 32760gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg 32820ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc 32880tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc 32940agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc 33000agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg 33060caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag 33120gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa 33180taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg 33240ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt 33300gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg 33360caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa 33420catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc 33480cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat 33540cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga 33600gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga 33660actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt 33720tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc 33780ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac 33840ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg 33900tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg 33960tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc 34020agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc 34080tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc 34140aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc 34200gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg 34260gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg 34320cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc 34380gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc 34440tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg 34500tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc 34560gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat 34620ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct 34680tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg 34740cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat 34800gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc 34860ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc 34920accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta 34980ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt gccgtgggcc 35040tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg 35100gcatcgttgc gccactcttc attaaccgct atatcgaaaa ttgcttgcgg cttgttagaa 35160ttgccatgac gtacctcggt gtcacgggta agattaccga taaactggaa ctgattatgg 35220ctcatatcga aagtctcctt gagaaaggag actctagttt agctaaacat tggttccgct 35280gtcaagaact ttagcggcta aaattttgcg ggccgcgacc aaaggtgcga ggggcggctt 35340ccgctgtgta caaccagata tttttcacca acatccttcg tctgctcgat gagcggggca 35400tgacgaaaca tgagctgtcg gagagggcag gggtttcaat ttcgttttta tcagacttaa 35460ccaacggtaa ggccaacccc tcgttgaagg tgatggaggc cattgccgac gccctggaaa 35520ctcccctacc tcttctcctg gagtccaccg accttgaccg cgaggcactc gcggagattg 35580cgggtcatcc tttcaagagc agcgtgccgc ccggatacga acgcatcagt gtggttttgc 35640cgtcacataa ggcgtttatc gtaaagaaat ggggcgacga cacccgaaaa aagctgcgtg 35700gaaggctctg acgccaaggg ttagggcttg cacttccttc tttagccgct aaaacggccc 35760cttctctgcg ggccgtcggc tcgcgcatca tatcgacatc ctcaacggaa gccgtgccgc 35820gaatggcatc gggcgggtgc gctttgacag ttgttttcta tcagaacccc tacgtcgtgc 35880ggttcgatta gctgtttgtc ttgcaggcta aacactttcg gtatatcgtt tgcctgtgcg 35940ataatgttgc taatgatttg ttgcgtaggg gttactgaaa agtgagcggg aaagaagagt 36000ttcagaccat caaggagcgg gccaagcgca agctggaacg cgacatgggt gcggacctgt 36060tggccgcgct caacgacccg aaaaccgttg aagtcatgct caacgcggac ggcaaggtgt 36120ggcacgaacg ccttggcgag ccgatgcggt acatctgcga catgcggccc agccagtcgc 36180aggcgattat agaaacggtg gccggattcc acggcaaaga ggtcacgcgg cattcgccca 36240tcctggaagg cgagttcccc ttggatggca gccgctttgc cggccaattg ccgccggtcg 36300tggccgcgcc aacctttgcg atccgcaagc gcgcggtcgc catcttcacg ctggaacagt 36360acgtcgaggc gggcatcatg acccgcgagc aatacgaggt cattaaaagc gccgtcgcgg 36420cgcatcgaaa catcctcgtc attggcggta ctggctcggg caagaccacg ctcgtcaacg 36480cgatcatcaa tgaaatggtc gccttcaacc cgtctgagcg cgtcgtcatc atcgaggaca 36540ccggcgaaat ccagtgcgcc gcagagaacg ccgtccaata ccacaccagc atcgacgtct 36600cgatgacgct gctgctcaag acaacgctgc gtatgcgccc cgaccgcatc ctggtcggtg 36660aggtacgtgg ccccgaagcc cttgatctgt tgatggcctg gaacaccggg catgaaggag 36720gtgccgccac cctgcacgca aacaacccca aagcgggcct gagccggctc gccatgctta 36780tcagcatgca cccggattca ccgaaaccca ttgagccgct gattggcgag gcggttcatg 36840tggtcgtcca tatcgccagg acccctagcg gccgtcgagt gcaagaaatt ctcgaagttc 36900ttggttacga gaacggccag tacatcacca aaaccctgta aggagtattt ccaatgacaa 36960cggctgttcc gttccgtctg accatgaatc gcggcatttt gttctacctt gccgtgttct 37020tcgttctcgc tctcgcgtta tccgcgcatc cggcgatggc ctcggaaggc accggcggca 37080gcttgccata tgagagctgg ctgacgaacc tgcgcaactc cgtaaccggc ccggtggcct 37140tcgcgctgtc catcatcggc atcgtcgtcg ccggcggcgt gctgatcttc ggcggcgaac 37200tcaacgcctt cttccgaacc ctgatcttcc tggttctggt gatggcgctg ctggtcggcg 37260cgcagaacgt gatgagcacc ttcttcggtc gtggtgccga aatcgcggcc ctcggcaacg 37320gggcgctgca ccaggtgcaa gtcgcggcgg cggatgccgt gcgtgcggta gcggctggac 37380ggctcgccta atcatggctc tgcgcacgat ccccatccgt cgcgcaggca accgagaaaa 37440cctgttcatg ggtggtgatc gtgaactggt gatgttctcg ggcctgatgg cgtttgcgct 37500gattttcagc gcccaagagc tgcgggccac cgtggtcggt ctgatcctgt ggttcggggc 37560gctctatgcg ttccgaatca tggcgaaggc cgatccgaag atgcggttcg tgtacctgcg 37620tcaccgccgg tacaagccgt attacccggc ccgctcgacc ccgttccgcg agaacaccaa 37680tagccaaggg aagcaatacc gatgatccaa gcaattgcga ttgcaatcgc gggcctcggc 37740gcgcttctgt tgttcatcct ctttgcccgc atccgcgcgg tcgatgccga actgaaactg 37800aaaaagcatc gttccaagga cgccggcctg gccgatctgc tcaactacgc cgctgtcgtc 37860gatgacggcg taatcgtggg caagaacggc agctttatgg ctgcctggct gtacaagggc 37920gatgacaacg caagcagcac cgaccagcag cgcgaagtag tgtccgcccg catcaaccag 37980gccctcgcgg gcctgggaag tgggtggatg atccatgtgg acgccgtgcg gcgtcctgct 38040ccgaactacg cggagcgggg cctgtcggcg ttccctgacc gtctgacggc agcgattgaa 38100gaagagcgct cggtcttgcc ttgctcgtcg gtgatgtact tcaccagctc cgcgaagtcg 38160ctcttcttga tggagcgcat ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt 38220tagcggctaa aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc 38280aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg aggatcgtgg catcaccgaa 38340ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc aggccgccca ggcggcccag 38400gtcgccattg atgcgggcca gctcgcggac gtgctcatag tccacgacgc ccgtgatttt 38460gtagccctgg ccgacggcca gcaggtaggc cgacaggctc atgccggccg ccgccgcctt 38520ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc ttgataggtg ggctgccctt 38580cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc 38640cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt 38700gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg 38760ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa 38820aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga 38880aaagcgctgc ttccctgctg ttttgtggaa tatctaccga ctggaaacag gcaaatgcag 38940gaaattactg aactgagggg acaggcgaga gacgatgcca aagagctaca ccgacgagct 39000ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg cgtgatgagg ctgcggttgc 39060gttcctggcg gtgagggcgg atgtcgaggc ggcgttagcg tccggctatg cgctcgtcac 39120catttgggag cacatgcggg aaacggggaa ggtcaagttc tcctacgaga cgttccgctc 39180gcacgccagg cggcacatca aggccaagcc cgccgatgtg cccgcaccgc aggccaaggc 39240tgcggaaccc gcgccggcac ccaagacgcc ggagccacgg cggccgaagc aggggggcaa 39300ggctgaaaag ccggcccccg ctgcggcccc gaccggcttc accttcaacc caacaccgga 39360caaaaaggat ctactgtaat ggcgaaaatt cacatggttt tgcagggcaa gggcggggtc 39420ggcaagtcgg ccatcgccgc gatcattgcg cagtacaaga tggacaaggg gcagacaccc 39480ttgtgcatcg acaccgaccc ggtgaacgcg acgttcgagg gctacaaggc cctgaacgtc 39540cgccggctga acatcatggc cggcgacgaa attaactcgc gcaacttcga caccctggtc 39600gagctgattg cgccgaccaa ggatgacgtg gtgatcgaca acggtgccag ctcgttcgtg 39660cctctgtcgc attacctcat cagcaaccag gtgccggctc tgctgcaaga aatggggcat 39720gagctggtca tccataccgt cgtcaccggc ggccaggctc tcctggacac ggtgagcggc 39780ttcgcccagc tcgccagcca gttcccggcc gaagcgcttt tcgtggtctg gctgaacccg 39840tattgggggc ctatcgagca tgagggcaag agctttgagc agatgaaggc gtacacggcc 39900aacaaggccc gcgtgtcgtc catcatccag attccggccc tcaaggaaga aacctacggc 39960cgcgatttca gcgacatgct gcaagagcgg ctgacgttcg accaggcgct ggccgatgaa 40020tcgctcacga tcatgacgcg gcaacgcctc aagatcgtgc ggcgcggcct gtttgaacag 40080ctcgacgcgg cggccgtgct atgagcgacc agattgaaga gctgatccgg gagattgcgg 40140ccaagcacgg catcgccgtc ggccgcgacg acccggtgct gatcctgcat accatcaacg 40200cccggctcat ggccgacagt gcggccaagc aagaggaaat ccttgccgcg ttcaaggaag 40260agctggaagg gatcgcccat cgttggggcg aggacgccaa ggccaaagcg gagcggatgc 40320tgaacgcggc cctggcggcc agcaaggacg caatggcgaa ggtaatgaag gacagcgccg 40380cgcaggcggc cgaagcgatc cgcagggaaa tcgacgacgg ccttggccgc cagctcgcgg 40440ccaaggtcgc ggacgcgcgg cgcgtggcga tgatgaacat gatcgccggc ggcatggtgt 40500tgttcgcggc cgccctggtg gtgtgggcct cgttatgaat cgcagaggcg cagatgaaaa 40560agcccggcgt tgccgggctt tgtttttgcg ttagctgggc ttgtttgaca ggcccaagct 40620ctgactgcgc ccgcgctcgc gctcctgggc ctgtttcttc tcctgctcct gcttgcgcat 40680cagggcctgg tgccgtcggg ctgcttcacg catcgaatcc cagtcgccgg ccagctcggg 40740atgctccgcg cgcatcttgc gcgtcgccag ttcctcgatc ttgggcgcgt gaatgcccat 40800gccttccttg atttcgcgca ccatgtccag ccgcgtgtgc agggtctgca agcgggcttg 40860ctgttgggcc tgctgctgct gccaggcggc ctttgtacgc ggcagggaca gcaagccggg 40920ggcattggac tgtagctgct gcaaacgcgc ctgctgacgg tctacgagct gttctaggcg 40980gtcctcgatg cgctccacct ggtcatgctt tgcctgcacg tagagcgcaa gggtctgctg 41040gtaggtctgc tcgatgggcg cggattctaa gagggcctgc tgttccgtct cggcctcctg 41100ggccgcctgt agcaaatcct cgccgctgtt gccgctggac tgctttactg ccggggactg 41160ctgttgccct gctcgcgccg tcgtcgcagt tcggcttgcc cccactcgat tgactgcttc 41220atttcgagcc gcagcgatgc gatctcggat tgcgtcaacg gacggggcag cgcggaggtg 41280tccggcttct ccttgggtga gtcggtcgat gccatagcca aaggtttcct tccaaaatgc 41340gtccattgct ggaccgtgtt tctcattgat gcccgcaagc atcttcggct tgaccgccag 41400gtcaagcgcg ccttcatggg cggtcatgac ggacgccgcc atgaccttgc cgccgttgtt 41460ctcgatgtag ccgcgtaatg aggcaatggt gccgcccatc gtcagcgtgt catcgacaac 41520gatgtacttc tggccgggga tcacctcccc ctcgaaagtc gggttgaacg ccaggcgatg 41580atctgaaccg gctccggttc gggcgacctt ctcccgctgc acaatgtccg tttcgacctc 41640aaggccaagg cggtcggcca gaacgaccgc catcatggcc ggaatcttgt tgttccccgc 41700cgcctcgacg gcgaggactg gaacgatgcg gggcttgtcg tcgccgatca gcgtcttgag 41760ctgggcaaca gtgtcgtccg aaatcaggcg ctcgaccaaa ttaagcgccg cttccgcgtc 41820gccctgcttc gcagcctggt attcaggctc gttggtcaaa gaaccaaggt cgccgttgcg 41880aaccaccttc gggaagtctc cccacggtgc gcgctcggct ctgctgtagc tgctcaagac 41940gcctcccttt ttagccgcta aaactctaac gagtgcgccc gcgactcaac ttgacgcttt 42000cggcacttac ctgtgccttg ccacttgcgt cataggtgat gcttttcgca ctcccgattt 42060caggtacttt atcgaaatct gaccgggcgt gcattacaaa gttcttcccc acctgttggt 42120aaatgctgcc gctatctgcg tggacgatgc tgccgtcgtg gcgctgcgac ttatcggcct 42180tttgggccat atagatgttg taaatgccag gtttcagggc cccggcttta tctaccttct 42240ggttcgtcca tgcgccttgg ttctcggtct ggacaattct ttgcccattc atgaccagga 42300ggcggtgttt cattgggtga ctcctgacgg ttgcctctgg tgttaaacgt gtcctggtcg 42360cttgccggct aaaaaaaagc cgacctcggc agttcgaggc cggctttccc tagagccggg 42420cgcgtcaagg ttgttccatc tattttagtg aactgcgttc gatttatcag ttactttcct 42480cccgctttgt gtttcctccc actcgtttcc gcgtctagcc gacccctcaa catagcggcc 42540tcttcttggg ctgcctttgc ctcttgccgc gcttcgtcac gctcggcttg caccgtcgta 42600aagcgctcgg cctgcctggc cgcctcttgc gccgccaact tcctttgctc ctggtgggcc 42660tcggcgtcgg cctgcgcctt cgctttcacc gctgccaact ccgtgcgcaa actctccgct 42720tcgcgcctgg tggcgtcgcg ctcgccgcga agcgcctgca tttcctggtt ggccgcgtcc 42780agggtcttgc ggctctcttc tttgaatgcg cgggcgtcct ggtgagcgta gtccagctcg 42840gcgcgcagct cctgcgctcg acgctccacc tcgtcggccc gctgcgtcgc cagcgcggcc 42900cgctgctcgg ctcctgccag ggcggtgcgt gcttcggcca gggcttgccg ctggcgtgcg 42960gccagctcgg ccgcctcggc ggcctgctgc tctagcaatg taacgcgcgc ctgggcttct 43020tccagctcgc gggcctgcgc ctcgaaggcg tcggccagct ccccgcgcac ggcttccaac 43080tcgttgcgct cacgatccca gccggcttgc gctgcctgca acgattcatt ggcaagggcc 43140tgggcggctt gccagagggc ggccacggcc tggttgccgg cctgctgcac cgcgtccggc 43200acctggactg ccagcggggc ggcctgcgcc gtgcgctggc gtcgccattc gcgcatgccg 43260gcgctggcgt cgttcatgtt gacgcgggcg gccttacgca ctgcatccac ggtcgggaag 43320ttctcccggt cgccttgctc gaacagctcg tccgcagccg caaaaatgcg gtcgcgcgtc 43380tctttgttca gttccatgtt ggctccggta attggtaaga ataataatac tcttacctac 43440cttatcagcg caagagttta gctgaacagt tctcgactta acggcaggtt ttttagcggc 43500tgaagggcag gcaaaaaaag ccccgcacgg tcggcggggg caaagggtca gcgggaaggg 43560gattagcggg cgtcgggctt cttcatgcgt cggggccgcg cttcttggga tggagcacga 43620cgaagcgcgc acgcgcatcg tcctcggccc tatcggcccg cgtcgcggtc aggaacttgt 43680cgcgcgctag gtcctccctg gtgggcacca ggggcatgaa ctcggcctgc tcgatgtagg 43740tccactccat gaccgcatcg cagtcgaggc cgcgttcctt caccgtctct tgcaggtcgc 43800ggtacgcccg ctcgttgagc ggctggtaac gggccaattg gtcgtaaatg gctgtcggcc 43860atgagcggcc tttcctgttg agccagcagc cgacgacgaa gccggcaatg caggcccctg 43920gcacaaccag gccgacgccg ggggcagggg atggcagcag ctcgccaacc aggaaccccg 43980ccgcgatgat gccgatgccg gtcaaccagc ccttgaaact atccggcccc gaaacacccc 44040tgcgcattgc ctggatgctg cgccggatag cttgcaacat caggagccgt ttcttttgtt 44100cgtcagtcat ggtccgccct caccagttgt tcgtatcggt gtcggacgaa ctgaaatcgc 44160aagagctgcc ggtatcggtc cagccgctgt ccgtgtcgct gctgccgaag cacggcgagg 44220ggtccgcgaa cgccgcagac ggcgtatccg gccgcagcgc atcgcccagc atggccccgg 44280tcagcgagcc gccggccagg tagcccagca tggtgctgtt ggtcgccccg gccaccaggg 44340ccgacgtgac gaaatcgccg tcattccctc tggattgttc gctgctcggc ggggcagtgc 44400gccgcgccgg cggcgtcgtg gatggctcgg gttggctggc ctgcgacggc cggcgaaagg 44460tgcgcagcag ctcgttatcg accggctgcg gcgtcggggc cgccgccttg cgctgcggtc 44520ggtgttcctt cttcggctcg cgcagcttga acagcatgat cgcggaaacc agcagcaacg 44580ccgcgcctac gcctcccgcg atgtagaaca gcatcggatt cattcttcgg tcctccttgt 44640agcggaaccg ttgtctgtgc ggcgcgggtg gcccgcgccg ctgtctttgg ggatcagccc 44700tcgatgagcg cgaccagttt cacgtcggca aggttcgcct cgaactcctg gccgtcgtcc 44760tcgtacttca accaggcata gccttccgcc ggcggccgac ggttgaggat aaggcgggca 44820gggcgctcgt cgtgctcgac ctggacgatg gcctttttca gcttgtccgg gtccggctcc 44880ttcgcgccct tttccttggc gtccttaccg tcctggtcgc cgtcctcgcc gtcctggccg 44940tcgccggcct ccgcgtcacg ctcggcatca gtctggccgt tgaaggcatc gacggtgttg 45000ggatcgcggc ccttctcgtc caggaactcg cgcagcagct tgaccgtgcc gcgcgtgatt 45060tcctgggtgt cgtcgtcaag ccacgcctcg acttcctccg ggcgcttctt gaaggccgtc 45120accagctcgt tcaccacggt cacgtcgcgc acgcggccgg tgttgaacgc atcggcgatc 45180ttctccggca ggtccagcag cgtgacgtgc tgggtgatga acgccggcga cttgccgatt 45240tccttggcga tatcgccttt cttcttgccc ttcgccagct cgcggccaat gaagtcggca 45300atttcgcgcg gggtcagctc gttgcgttgc aggttctcga taacctggtc ggcttcgttg 45360tagtcgttgt cgatgaacgc cgggatggac ttcttgccgg cccacttcga gccacggtag 45420cggcgggcgc cgtgattgat gatatagcgg cccggctgct cctggttctc gcgcaccgaa 45480atgggtgact tcaccccgcg ctctttgatc gtggcaccga tttccgcgat gctctccggg 45540gaaaagccgg ggttgtcggc cgtccgcggc tgatgcggat cttcgtcgat caggtccagg 45600tccagctcga tagggccgga accgccctga gacgccgcag gagcgtccag gaggctcgac 45660aggtcgccga tgctatccaa ccccaggccg gacggctgcg ccgcgcctgc ggcttcctga 45720gcggccgcag cggtgttttt cttggtggtc ttggcttgag ccgcagtcat tgggaaatct 45780ccatcttcgt gaacacgtaa tcagccaggg cgcgaacctc tttcgatgcc ttgcgcgcgg 45840ccgttttctt gatcttccag accggcacac cggatgcgag ggcatcggcg atgctgctgc 45900gcaggccaac ggtggccgga atcatcatct tggggtacgc ggccagcagc tcggcttggt 45960ggcgcgcgtg gcgcggattc cgcgcatcga ccttgctggg caccatgcca aggaattgca 46020gcttggcgtt cttctggcgc acgttcgcaa tggtcgtgac catcttcttg atgccctgga 46080tgctgtacgc ctcaagctcg atgggggaca gcacatagtc ggccgcgaag agggcggccg 46140ccaggccgac gccaagggtc ggggccgtgt cgatcaggca cacgtcgaag ccttggttcg 46200ccagggcctt gatgttcgcc ccgaacagct cgcgggcgtc gtccagcgac agccgttcgg 46260cgttcgccag taccgggttg gactcgatga gggcgaggcg cgcggcctgg ccgtcgccgg 46320ctgcgggtgc ggtttcggtc cagccgccgg cagggacagc gccgaacagc ttgcttgcat 46380gcaggccggt agcaaagtcc ttgagcgtgt aggacgcatt gccctggggg tccaggtcga 46440tcacggcaac ccgcaagccg cgctcgaaaa agtcgaaggc aagatgcaca agggtcgaag 46500tcttgccgac gccgcctttc tggttggccg tgaccaaagt tttcatcgtt tggtttcctg 46560ttttttcttg gcgtccgctt cccacttccg gacgatgtac gcctgatgtt ccggcagaac 46620cgccgttacc cgcgcgtacc cctcgggcaa gttcttgtcc tcgaacgcgg cccacacgcg 46680atgcaccgct tgcgacactg cgcccctggt cagtcccagc gacgttgcga acgtcgcctg 46740tggcttccca tcgactaaga cgccccgcgc tatctcgatg gtctgctgcc ccacttccag 46800cccctggatc gcctcctgga actggctttc ggtaagccgt ttcttcatgg ataacaccca 46860taatttgctc cgcgccttgg ttgaacatag cggtgacagc cgccagcaca tgagagaagt 46920ttagctaaac atttctcgca cgtcaacacc tttagccgct aaaactcgtc cttggcgtaa 46980caaaacaaaa gcccggaaac cgggctttcg tctcttgccg cttatggctc tgcacccggc 47040tccatcacca acaggtcgcg cacgcgcttc actcggttgc ggatcgacac tgccagccca 47100acaaagccgg ttgccgccgc cgccaggatc gcgccgatga tgccggccac accggccatc 47160gcccaccagg tcgccgcctt ccggttccat tcctgctggt actgcttcgc aatgctggac 47220ctcggctcac cataggctga ccgctcgatg gcgtatgccg cttctcccct tggcgtaaaa 47280cccagcgccg caggcggcat tgccatgctg cccgccgctt tcccgaccac gacgcgcgca 47340ccaggcttgc ggtccagacc ttcggccacg gcgagctgcg caaggacata atcagccgcc 47400gacttggctc cacgcgcctc gatcagctct tgcactcgcg cgaaatcctt ggcctccacg 47460gccgccatga atcgcgcacg cggcgaaggc tccgcagggc cggcgtcgtg atcgccgccg 47520agaatgccct tcaccaagtt cgacgacacg aaaatcatgc tgacggctat

caccatcatg 47580cagacggatc gcacgaaccc gctgaattga acacgagcac ggcacccgcg accactatgc 47640caagaatgcc caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat gccccgacgg 47700ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc actgcccggc acctggtcgc 47760tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga 47820tcgcccatcc cgttactgcc ccgatcccgg caatggcaag gactgccagc gctgccattt 47880ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt 47940tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg 48000cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa 48060aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg 48120cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc 48180tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac 48240cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag 48300gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc 48360ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct 48420catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt 48480ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc 48540ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgctggaagc cccgtagcga 48600cgcggagagg ggcgagacaa gccaagggcg caggctcgat gcgcagcacg acatagccgg 48660ttctcgcaag gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa agtttccaac 48720gcgagccatt cgcgagagcc ttgagtccac gctagatgag agctttgttg taggtggacc 48780agttggtgat tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg 48840tgatctgatc cttcaactca gcaaaagttc gatttattca acaaagccac gttgtgtctc 48900aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt 48960ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaac 490159648997DNAArtificial SeqeunceVector 96gtcttgctcg actctagagc tcgttcctcg aggcctcgag gcctcgagga acggtacctg 60cggggaagct tacaataatg tgtgttgtta agtcttgttg cctgtcatcg tctgactgac 120tttcgtcata aatcccggcc tccgtaaccc agctttgggc aagctcacgg atttgatccg 180gcggaacggg aatatcgaga tgccgggctg aacgctgcag ttccagcttt ccctttcggg 240acaggtactc cagctgattg attatctgct gaagggtctt ggttccacct cctggcacaa 300tgcgaatgat tacttgagcg cgatcgggca tccaattttc tcccgtcagg tgcgtggtca 360agtgctacaa ggcacctttc agtaacgagc gaccgtcgat ccgtcgccgg gatacggaca 420aaatggagcg cagtagtcca tcgagggcgg cgaaagcctc gccaaaagca atacgttcat 480ctcgcacagc ctccagatcc gatcgagggt cttcggcgta ggcagataga agcatggata 540cattgcttga gagtattccg atggactgaa gtatggcttc catcttttct cgtgtgtctg 600catctatttc gagaaagccc ccgatgcggc gcaccgcaac gcgaattgcc atactatccg 660aaagtcccag caggcgcgct tgataggaaa aggtttcata ctcggccgat cgcagacggg 720cactcacgac cttgaaccct tcaactttca gggatcgatg ctggttgatg gtagtctcac 780tcgacgtggc tctggtgtgt tttgacatag cttcctccaa agaaagcgga aggtctggat 840actccagcac gaaatgtgcc cgggtagacg gatggaagtc tagccctgct caatatgaaa 900tcaacagtac atttacagtc aatactgaat atacttgcta catttgcaat tgtcttataa 960cgaatgtgaa ataaaaatag tgtaacaacg cttttactca tcgataatca caaaaacatt 1020tatacgaaca aaaatacaaa tgcactccgg tttcacagga taggcgggat cagaatatgc 1080aacttttgac gttttgttct ttcaaagggg gtgctggcaa aaccaccgca ctcatgggcc 1140tttgcgctgc tttggcaaat gacggtaaac gagtggccct ctttgatgcc gacgaaaacc 1200ggcctctgac gcgatggaga gaaaacgcct tacaaagcag tactgggatc ctcgctgtga 1260agtctattcc gccgacgaaa tgccccttct tgaagcagcc tatgaaaatg ccgagctcga 1320aggatttgat tatgcgttgg ccgatacgcg tggcggctcg agcgagctca acaacacaat 1380catcgctagc tcaaacctgc ttctgatccc caccatgcta acgccgctcg acatcgatga 1440ggcactatct acctaccgct acgtcatcga gctgctgttg agtgaaaatt tggcaattcc 1500tacagctgtt ttgcgccaac gcgtcccggt cggccgattg acaacatcgc aacgcaggat 1560gtcagagacg ctagagagcc ttccagttgt accgtctccc atgcatgaaa gagatgcatt 1620tgccgcgatg aaagaacgcg gcatgttgca tcttacatta ctaaacacgg gaactgatcc 1680gacgatgcgc ctcatagaga ggaatcttcg gattgcgatg gaggaagtcg tggtcatttc 1740gaaactgatc agcaaaatct tggaggcttg aagatggcaa ttcgcaagcc cgcattgtcg 1800gtcggcgaag cacggcggct tgctggtgct cgacccgaga tccaccatcc caacccgaca 1860cttgttcccc agaagctgga cctccagcac ttgcctgaaa aagccgacga gaaagaccag 1920caacgtgagc ctctcgtcgc cgatcacatt tacagtcccg atcgacaact taagctaact 1980gtggatgccc ttagtccacc tccgtccccg aaaaagctcc aggtttttct ttcagcgcga 2040ccgcccgcgc ctcaagtgtc gaaaacatat gacaacctcg ttcggcaata cagtccctcg 2100aagtcgctac aaatgatttt aaggcgcgcg ttggacgatt tcgaaagcat gctggcagat 2160ggatcatttc gcgtggcccc gaaaagttat ccgatccctt caactacaga aaaatccgtt 2220ctcgttcaga cctcacgcat gttcccggtt gcgttgctcg aggtcgctcg aagtcatttt 2280gatccgttgg ggttggagac cgctcgagct ttcggccaca agctggctac cgccgcgctc 2340gcgtcattct ttgctggaga gaagccatcg agcaattggt gaagagggac ctatcggaac 2400ccctcaccaa atattgagtg taggtttgag gccgctggcc gcgtcctcag tcaccttttg 2460agccagataa ttaagagcca aatgcaattg gctcaggctg ccatcgtccc cccgtgcgaa 2520acctgcacgt ccgcgtcaaa gaaataaccg gcacctcttg ctgtttttat cagttgaggg 2580cttgacggat ccgcctcaag tttgcggcgc agccgcaaaa tgagaacatc tatactcctg 2640tcgtaaacct cctcgtcgcg tactcgactg gcaatgagaa gttgctcgcg cgatagaacg 2700tcgcggggtt tctctaaaaa cgcgaggaga agattgaact cacctgccgt aagtttcacc 2760tcaccgccag cttcggacat caagcgacgt tgcctgagat taagtgtcca gtcagtaaaa 2820caaaaagacc gtcggtcttt ggagcggaca acgttggggc gcacgcgcaa ggcaacccga 2880atgcgtgcaa gaaactctct cgtactaaac ggcttagcga taaaatcact tgctcctagc 2940tcgagtgcaa caactttatc cgtctcctca aggcggtcgc cactgataat tatgattgga 3000atatcagact ttgccgccag atttcgaacg atctcaagcc catcttcacg acctaaattt 3060agatcaacaa ccacgacatc gaccgtcgcg gaagagagta ctctagtgaa ctgggtgctg 3120tcggctaccg cggtcacttt gaaggcgtgg atcgtaaggt attcgataat aagatgccgc 3180atagcgacat cgtcatcgat aagaagaacg tgtttcaacg gctcaccttt caatctaaaa 3240tctgaaccct tgttcacagc gcttgagaaa ttttcacgtg aaggatgtac aatcatctcc 3300agctaaatgg gcagttcgtc agaattgcgg ctgaccgcgg atgacgaaaa tgcgaaccaa 3360gtatttcaat tttatgacaa aagttctcaa tcgttgttac aagtgaaacg cttcgaggtt 3420acagctacta ttgattaagg agatcgccta tggtctcgcc ccggcgtcgt gcgtccgccg 3480cgagccagat ctcgcctact tcataaacgt cctcataggc acggaatgga atgatgacat 3540cgatcgccgt agagagcatg tcaatcagtg tgcgatcttc caagctagca ccttgggcgc 3600tacttttgac aagggaaaac agtttcttga atccttggat tggattcgcg ccgtgtattg 3660ttgaaatcga tcccggatgt cccgagacga cttcactcag ataagcccat gctgcatcgt 3720cgcgcatctc gccaagcaat atccggtccg gccgcatacg cagacttgct tggagcaagt 3780gctcggcgct cacagcaccc agcccagcac cgttcttgga gtagagtagt ctaacatgat 3840tatcgtgtgg aatgacgagt tcgagcgtat cttctatggt gattagcctt tcctgggggg 3900ggatggcgct gatcaaggtc ttgctcattg ttgtcttgcc gcttccggta gggccacata 3960gcaacatcgt cagtcggctg acgacgcatg cgtgcagaaa cgcttccaaa tccccgttgt 4020caaaatgctg aaggatagct tcatcatcct gattttggcg tttccttcgt gtctgccact 4080ggttccacct cgaagcatca taacgggagg agacttcttt aagaccagaa acacgcgagc 4140ttggccgtcg aatggtcaag ctgacggtgc ccgagggaac ggtcggcggc agacagattt 4200gtagtcgttc accaccagga agttcagtgg cgcagagggg gttacgtggt ccgacatcct 4260gctttctcag cgcgcccgct aaaatagcga tatcttcaag atcatcataa gagacgggca 4320aaggcatctt ggtaaaaatg ccggcttggc gcacaaatgc ctctccaggt cgattgatcg 4380caatttcttc agtcttcggg tcatcgagcc attccaaaat cggcttcaga agaaagcgta 4440gttgcggatc cacttccatt tacaatgtat cctatctcta agcggaaatt tgaattcatt 4500aagagcggcg gttcctcccc cgcgtggcgc cgccagtcag gcggagctgg taaacaccaa 4560agaaatcgag gtcccgtgct acgaaaatgg aaacggtgtc accctgattc ttcttcaggg 4620ttggcggtat gttgatggtt gccttaaggg ctgtctcagt tgtctgctca ccgttatttt 4680gaaagctgtt gaagctcatc ccgccacccg agctgccggc gtaggtgcta gctgcctgga 4740aggcgccttg aacaacactc aagagcatag ctccgctaaa acgctgccag aagtggctgt 4800cgaccgagcc cggcaatcct gagcgaccga gttcgtccgc gcttggcgat gttaacgaga 4860tcatcgcatg gtcaggtgtc tcggcgcgat cccacaacac aaaaacgcgc ccatctccct 4920gttgcaagcc acgctgtatt tcgccaacaa cggtggtgcc acgatcaaga agcacgatat 4980tgttcgttgt tccacgaata tcctgaggca agacacactt tacatagcct gccaaatttg 5040tgtcgattgc ggtttgcaag atgcacggaa ttattgtccc ttgcgttacc ataaaatcgg 5100ggtgcggcaa gagcgtggcg ctgctgggct gcagctcggt gggtttcata cgtatcgaca 5160aatcgttctc gccggacact tcgccattcg gcaaggagtt gtcgtcacgc ttgccttctt 5220gtcttcggcc cgtgtcgccc tgaatggcgc gtttgctgac cccttgatcg ccgctgctat 5280atgcaaaaat cggtgtttct tccggccgtg gctcatgccg ctccggttcg cccctcggcg 5340gtagaggagc agcaggctga acagcctctt gaaccgctgg aggatccggc ggcacctcaa 5400tcggagctgg atgaaatggc ttggtgtttg ttgcgatcaa agttgacggc gatgcgttct 5460cattcacctt cttttggcgc ccacctagcc aaatgaggct taatgataac gcgagaacga 5520cacctccgac gatcaatttc tgagaccccg aaagacgccg gcgatgtttg tcggagacca 5580gggatccaga tgcatcaacc tcatgtgccg cttgctgact atcgttattc atcccttcgc 5640ccccttcagg acgcgtttca catcgggcct caccgtgccc gtttgcggcc tttggccaac 5700gggatcgtaa gcggtgttcc agatacatag tactgtgtgg ccatccctca gacgccaacc 5760tcgggaaacc gaagaaatct cgacatcgct ccctttaact gaatagttgg caacagcttc 5820cttgccatca ggattgatgg tgtagatgga gggtatgcgt acattgcccg gaaagtggaa 5880taccgtcgta aatccattgt cgaagacttc gagtggcaac agcgaacgat cgccttgggc 5940gacgtagtgc caattactgt ccgccgcacc aagggctgtg acaggctgat ccaataaatt 6000ctcagctttc cgttgatatt gtgcttccgc gtgtagtctg tccacaacag ccttctgttg 6060tgcctccctt cgccgagccg ccgcatcgtc ggcggggtag gcgaattgga cgctgtaata 6120gagatcgggc tgctctttat cgaggtggga cagagtcttg gaacttatac tgaaaacata 6180acggcgcatc ccggagtcgc ttgcggttag cacgattact ggctgaggcg tgaggacctg 6240gcttgccttg aaaaatagat aatttccccg cggtagggct gctagatctt tgctatttga 6300aacggcaacc gctgtcaccg tttcgttcgt ggcgaatgtt acgaccaaag tagctccaac 6360cgccgtcgag aggcgcacca cttgatcggg attgtaagcc aaataacgca tgcgcggatc 6420tagcttgccc gccattggag tgtcttcagc ctccgcacca gtcgcagcgg caaataaaca 6480tgctaaaatg aaaagtgctt ttctgatcat ggttcgctgt ggcctacgtt tgaaacggta 6540tcttccgatg tctgatagga ggtgacaacc agacctgccg ggttggttag tctcaatctg 6600ccgggcaagc tggtcacctt ttcgtagcga actgtcgcgg tccacgtact caccacaggc 6660attttgccgt caacgacgag ggtcctttta tagcgaattt gctgcgtgct tggagttaca 6720tcatttgaag cgatgtgctc gacctccacc ctgccgcgtt tgccaagaat gacttgaggc 6780gaactgggat tgggatagtt gaagaattgc tggtaatcct ggcgcactgt tggggcactg 6840aagttcgata ccaggtcgta ggcgtactga gcggtgtcgg catcataact ctcgcgcagg 6900cgaacgtact cccacaatga ggcgttaacg acggcctcct cttgagttgc aggcaatcgc 6960gagacagaca cctcgctgtc aacggtgccg tccggccgta tccatagata tacgggcaca 7020agcctgctca acggcaccat tgtggctata gcgaacgctt gagcaacatt tcccaaaatc 7080gcgatagctg cgacagctgc aatgagtttg gagagacgtc gcgccgattt cgctcgcgcg 7140gtttgaaagg cttctacttc cttatagtgc tcggcaaggc tttcgcgcgc cactagcatg 7200gcatattcag gccccgtcat agcgtccacc cgaattgccg agctgaagat ctgacggagt 7260aggctgccat cgccccacat tcagcgggaa gatcgggcct ttgcagctcg ctaatgtgtc 7320gtttgtctgg cagccgctca aagcgacaac taggcacagc aggcaatact tcatagaatt 7380ctccattgag gcgaattttt gcgcgaccta gcctcgctca acctgagcga agcgacggta 7440caagctgctg gcagattggg ttgcgccgct ccagtaactg cctccaatgt tgccggcgat 7500cgccggcaaa gcgacaatga gcgcatcccc tgtcagaaaa aacatatcga gttcgtaaag 7560accaatgatc ttggccgcgg tcgtaccggc gaaggtgatt acaccaagca taagggtgag 7620cgcagtcgct tcggttagga tgacgatcgt tgccacgagg tttaagagga gaagcaagag 7680accgtaggtg ataagttgcc cgatccactt agctgcgatg tcccgcgtgc gatcaaaaat 7740atatccgacg aggatcagag gcccgatcgc gagaagcact ttcgtgagaa ttccaacggc 7800gtcgtaaact ccgaaggcag accagagcgt gccgtaaagg acccactgtg ccccttggaa 7860agcaaggatg tcctggtcgt tcatcggacc gatttcggat gcgattttct gaaaaacggc 7920ctgggtcacg gcgaacattg tatccaactg tgccggaaca gtctgcagag gcaagccggt 7980tacactaaac tgctgaacaa agtttgggac cgtcttttcg aagatggaaa ccacatagtc 8040ttggtagtta gcctgcccaa caattagagc aacaacgatg gtgaccgtga tcacccgagt 8100gataccgcta cgggtatcga cttcgccgcg tatgactaaa ataccctgaa caataatcca 8160aagagtgaca caggcgatca atggcgcact caccgcctcc tggatagtct caagcatcga 8220gtccaagcct gtcgtgaagg ctacatcgaa gatcgtatga atggccgtaa acggcgccgg 8280aatcgtgaaa ttcatcgatt ggacctgaac ttgactggtt tgtcgcataa tgttggataa 8340aatgagctcg cattcggcga ggatgcgggc ggatgaacaa atcgcccagc cttaggggag 8400ggcaccaaag atgacagcgg tcttttgatg ctccttgcgt tgagcggccg cctcttccgc 8460ctcgtgaagg ccggcctgcg cggtagtcat cgttaatagg cttgtcgcct gtacattttg 8520aatcattgcg tcatggatct gcttgagaag caaaccattg gtcacggttg cctgcatgat 8580attgcgagat cgggaaagct gagcagacgt atcagcattc gccgtcaagc gtttgtccat 8640cgtttccaga ttgtcagccg caatgccagc gctgtttgcg gaaccggtga tctgcgatcg 8700caacaggtcc gcttcagcat cactacccac gactgcacga tctgtatcgc tggtgatcgc 8760acgtgccgtg gtcgacattg gcattcgcgg cgaaaacatt tcattgtcta ggtccttcgt 8820cgaaggatac tgatttttct ggttgagcga agtcagtagt ccagtaacgc cgtaggccga 8880cgtcaacatc gtaaccatcg ctatagtctg agtgagattc tccgcagtcg cgagcgcagt 8940cgcgagcgtc tcagcctccg ttgccgggtc gctaacaaca aactgcgccc gcgcgggctg 9000aatatataga aagctgcagg tcaaaactgt tgcaataagt tgcgtcgtct tcatcgtttc 9060ctaccttatc aatcttctgc ctcgtggtga cgggccatga attcgctgag ccagccagat 9120gagttgcctt cttgtgcctc gcgtagtcga gttgcaaagc gcaccgtgtt ggcacgcccc 9180gaaagcacgg cgacatattc acgcatatcc cgcagatcaa attcgcagat gacgcttcca 9240ctttctcgtt taagaagaaa cttacggctg ccgaccgtca tgtcttcacg gatcgcctga 9300aattcctttt cggtacattt cagtccatcg acataagccg atcgatctgc ggttggtgat 9360ggatagaaaa tcttcgtcat acattgcgca accaagctgg ctcctagcgg cgattccaga 9420acatgctctg gttgctgcgt tgccagtatt agcatcccgt tgttttttcg aacggtcagg 9480aggaatttgt cgacgacagt cgaaaattta gggtttaaca aataggcgcg aaactcatcg 9540cagctcatca caaaacggcg gccgtcgatc atggctccaa tccgatgcag gagatatgct 9600gcagcgggag cgcatacttc ctcgtattcg agaagatgcg tcatgtcgaa gccggtaatc 9660gacggatcta actttacttc gtcaacttcg ccgtcaaatg cccagccaag cgcatggccc 9720cggcaccagc gttggagccg cgctcctgcg ccttcggcgg gcccatgcaa caaaaattca 9780cgtaaccccg cgattgaacg catttgtgga tcaaacgaga gctgacgatg gataccacgg 9840accagacggc ggttctcttc cggagaaatc ccaccccgac catcactctc gatgagagcc 9900acgatccatt cgcgcagaaa atcgtgtgag gctgctgtgt tttctaggcc acgcaacggc 9960gccaacccgc tgggtgtgcc tctgtgaagt gccaaatatg ttcctcctgt ggcgcgaacc 10020agcaattcgc caccccggtc cttgtcaaag aacacgaccg tacctgcacg gtcgaccatg 10080ctctgttcga gcatggctag aacaaacatc atgagcgtcg tcttacccct cccgataggc 10140ccgaatattg ccgtcatgcc aacatcgtgc tcatgcggga tatagtcgaa aggcgttccg 10200ccattggtac gaaatcgggc aatcgcgttg ccccagtggc ctgagctggc gccctctgga 10260aagttttcga aagagacaaa ccctgcgaaa ttgcgtgaag tgattgcgcc agggcgtgtg 10320cgccacttaa aattccccgg caattgggac caataggccg cttccatacc aataccttct 10380tggacaacca cggcacctgc atccgccatt cgtgtccgag cccgcgcgcc cctgtcccca 10440agactattga gatcgtctgc atagacgcaa aggctcaaat gatgtgagcc cataacgaat 10500tcgttgctcg caagtgcgtc ctcagcctcg gataatttgc cgatttgagt cacggcttta 10560tcgccggaac tcagcatctg gctcgatttg aggctaagtt tcgcgtgcgc ttgcgggcga 10620gtcaggaacg aaaaactctg cgtgagaaca agtggaaaat cgagggatag cagcgcgttg 10680agcatgcccg gccgtgtttt tgcagggtat tcgcgaaacg aatagatgga tccaacgtaa 10740ctgtcttttg gcgttctgat ctcgagtcct cgcttgccgc aaatgactct gtcggtataa 10800atcgaagcgc cgagtgagcc gctgacgacc ggaaccggtg tgaaccgacc agtcatgatc 10860aaccgtagcg cttcgccaat ttcggtgaag agcacaccct gcttctcgcg gatgccaaga 10920cgatgcaggc catacgcttt aagagagcca gcgacaacat gccaaagatc ttccatgttc 10980ctgatctggc ccgtgagatc gttttccctt tttccgctta gcttggtgaa cctcctcttt 11040accttcccta aagccgcctg tgggtagaca atcaacgtaa ggaagtgttc attgcggagg 11100agttggccgg agagcacgcg ctgttcaaaa gcttcgttca ggctagcggc gaaaacacta 11160cggaagtgtc gcggcgccga tgatggcacg tcggcatgac gtacgaggtg agcatatatt 11220gacacatgat catcagcgat attgcgcaac agcgtgttga acgcacgaca acgcgcattg 11280cgcatttcag tttcctcaag ctcgaatgca acgccatcaa ttctcgcaat ggtcatgatc 11340gatccgtctt caagaaggac gatatggtcg ctgaggtggc caatataagg gagatagatc 11400tcaccggatc tttcggtcgt tccactcgcg ccgagcatca caccattcct ctccctcgtg 11460ggggaaccct aattggattt gggctaacag tagcgccccc ccaaactgca ctatcaatgc 11520ttcttcccgc ggtccgcaaa aatagcagga cgacgctcgc cgcattgtag tctcgctcca 11580cgatgagccg ggctgcaaac cataacggca cgagaacgac ttcgtagagc gggttctgaa 11640cgataacgat gacaaagccg gcgaacatca tgaataaccc tgccaatgtc agtggcaccc 11700caagaaacaa tgcgggccgt gtggctgcga ggtaaagggt cgattcttcc aaacgatcag 11760ccatcaacta ccgccagtga gcgtttggcc gaggaagctc gccccaaaca tgataacaat 11820gccgccgacg acgccggcaa ccagcccaag cgaagcccgc ccgaacatcc aggagatccc 11880gatagcgaca atgccgagaa cagcgagtga ctggccgaac ggaccaagga taaacgtgca 11940tatattgtta accattgtgg cggggtcagt gccgccaccc gcagattgcg ctgcggcggg 12000tccggatgag gaaatgctcc atgcaattgc accgcacaag cttggggcgc agctcgatat 12060cacgcgcatc atcgcattcg agagcgagag gcgatttaga tgtaaacggt atctctcaaa 12120gcatcgcatc aatgcgcacc tccttagtat aagtcgaata agacttgatt gtcgtctgcg 12180gatttgccgt tgtcctggtg tggcggtggc ggagcgatta aaccgccagc gccatcctcc 12240tgcgagcggc gctgatatga cccccaaaca tcccacgtct cttcggattt tagcgcctcg 12300tgatcgtctt ttggaggctc gattaacgcg ggcaccagcg attgagcagc tgtttcaact 12360tttcgcacgt agccgtttgc aaaaccgccg atgaaattac cggtgttgta agcggagatc 12420gcccgacgaa gcgcaaattg cttctcgtca atcgtttcgc cgcctgcata acgacttttc 12480agcatgtttg cagcggcaga taatgatgtg cacgcctgga gcgcaccgtc aggtgtcaga 12540ccgagcatag aaaaatttcg agagtttatt tgcatgaggc caacatccag cgaatgccgt 12600gcatcgagac ggtgcctgac gacttgggtt gcttggctgt gatcttgcca gtgaagcgtt 12660tcgccggtcg tgttgtcatg aatcgctaaa ggatcaaagc gactctccac cttagctatc 12720gccgcaagcg tagatgtcgc aactgatggg gcacacttgc gagcaacatg gtcaaactca 12780gcagatgaga gtggcgtggc aaggctcgac gaacagaagg agaccatcaa ggcaagagaa 12840agcgaccccg atctcttaag cataccttat ctccttagct cgcaactaac accgcctctc 12900ccgttggaag aagtgcgttg ttttatgttg aagattatcg ggagggtcgg ttactcgaaa 12960attttcaatt gcttctttat gatttcaatt gaagcgagaa acctcgcccg gcgtcttgga 13020acgcaacatg gaccgagaac cgcgcatcca tgactaagca accggatcga cctattcagg 13080ccgcagttgg tcaggtcagg ctcagaacga aaatgctcgg cgaggttacg ctgtctgtaa 13140acccattcga tgaacgggaa gcttccttcc gattgctctt ggcaggaata ttggcccatg 13200cctgcttgcg ctttgcaaat gctcttatcg cgttggtatc atatgccttg tccgccagca 13260gaaacgcact ctaagcgatt atttgtaaaa atgtttcggt catgcggcgg tcatgggctt 13320gacccgctgt cagcgcaaga cggatcggtc aaccgtcggc atcgacaaca gcgtgaatct 13380tggtggtcaa accgccacgg gaacgtccca tacagccatc gtcttgatcc cgctgtttcc 13440cgtcgccgca tgttggtgga cgcggacaca ggaactgtca atcatgacga cattctatcg 13500aaagccttgg aaatcacact cagaatatga tcccagacgt ctgcctcacg ccatcgtaca 13560aagcgattgt

agcaggttgt acaggaaccg tatcgatcag gaacgtctgc ccagggcggg 13620cccgtccgga agcgccacaa gatgacattg atcacccgcg tcaacgcgcg gcacgcgacg 13680cggcttattt gggaacaaag gactgaacaa cagtccattc gaaatcggtg acatcaaagc 13740ggggacgggt tatcagtggc ctccaagtca agcctcaatg aatcaaaatc agaccgattt 13800gcaaacctga tttatgagtg tgcggcctaa atgatgaaat cgtccttcta gatcgcctcc 13860gtggtgtagc aacacctcgc agtatcgccg tgctgacctt ggccagggaa ttgactggca 13920agggtgcttt cacatgaccg ctcttttggc cgcgatagat gatttcgttg ctgctttggg 13980cacgtagaag gagagaagtc atatcggaga aattcctcct ggcgcgagag cctgctctat 14040cgcgacggca tcccactgtc gggaacagac cggatcattc acgaggcgaa agtcgtcaac 14100acatgcgtta taggcatctt cccttgaagg atgatcttgt tgctgccaat ctggaggtgc 14160ggcagccgca ggcagatgcg atctcagcgc aacttgcggc aaaacatctc actcacctga 14220aaaccactag cgagtctcgc gatcagacga aggcctttta cttaacgaca caatatccga 14280tgtctgcatc acaggcgtcg ctatcccagt caatactaaa gcggtgcagg aactaaagat 14340tactgatgac ttaggcgtgc cacgaggcct gagacgacgc gcgtagacag ttttttgaaa 14400tcattatcaa agtgatggcc tccgctgaag cctatcacct ctgcgccggt ctgtcggaga 14460gatgggcaag cattattacg gtcttcgcgc ccgtacatgc attggacgat tgcagggtca 14520atggatctga gatcatccag aggattgccg cccttacctt ccgtttcgag ttggagccag 14580cccctaaatg agacgacata gtcgacttga tgtgacaatg ccaagagaga gatttgctta 14640acccgatttt tttgctcaag cgtaagccta ttgaagcttg ccggcatgac gtccgcgccg 14700aaagaatatc ctacaagtaa aacattctgc acaccgaaat gcttggtgta gacatcgatt 14760atgtgaccaa gatccttagc agtttcgctt ggggaccgct ccgaccagaa ataccgaagt 14820gaactgacgc caatgacagg aatcccttcc gtctgcagat aggtaccatc gatagatctg 14880ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 14940ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 15000gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 15060tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 15120gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 15180ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 15240gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 15300ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 15360cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 15420ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 15480accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 15540catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 15600gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 15660tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 15720agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 15780actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 15840gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 15900aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 15960gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 16020aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 16080atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 16140gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 16200atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 16260ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 16320cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 16380agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggggg gggggggggg 16440gggttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag 16500ctcgctttca gcacctgtcg tttcctttct tttcagaggg tattttaaat aaaaacatta 16560agttatgacg aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa 16620cccgcgaggt ccctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct 16680acatatcaca acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg 16740tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca 16800gcgacactga atacggggca acctcatgtc cccccccccc ccccccctgc aggcatcgtg 16860gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 16920gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 16980gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17040cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17100ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17160accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 17220aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 17280aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 17340caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 17400ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 17460gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 17520cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 17580aggccctttc gtcttcaaga attggtcgac gatcttgctg cgttcggata ttttcgtgga 17640gttcccgcca cagacccgga ttgaaggcga gatccagcaa ctcgcgccag atcatcctgt 17700gacggaactt tggcgcgtga tgactggcca ggacgtcggc cgaaagagcg acaagcagat 17760cacgcttttc gacagcgtcg gatttgcgat cgaggatttt tcggcgctgc gctacgtccg 17820cgaccgcgtt gagggatcaa gccacagcag cccactcgac cttctagccg acccagacga 17880gccaagggat ctttttggaa tgctgctccg tcgtcaggct ttccgacgtt tgggtggttg 17940aacagaagtc attatcgtac ggaatgccaa gcactcccga ggggaaccct gtggttggca 18000tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc 18060taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt 18120ttaaactgaa ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt 18180atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa 18240cgttgaagga gccactcagc aagctggtac gattgtaata cgactcacta tagggcgaat 18300tgagcgctgt ttaaacgctc ttcaactgga agagcggtta ccagagctgg tcacctttgt 18360ccaccaagat ggaactgcgg ccgctcatta attaagtcag gcgcgcctct agttgaagac 18420acgttcatgt cttcatcgta agaagacact cagtagtctt cggccagaat ggccatctgg 18480attcagcagg cctagaaggc catttaaatc ctgaggatct ggtcttccta aggacccggg 18540atatcgctat caactttgta tagaaaagtt gggccgaatt cgcccttgtt taaacttaat 18600atttgtttaa actttttact aaattcatgt aataattaat gtatgcgtta tatatatatg 18660tctaggttta taattattca tatgaatatg aacataaaaa tctagggcta aaacgactac 18720tattttgaaa acggaaggag tagtaagtta tttaagcgga ggggaaccat gatgggctag 18780tgatttaatt tacatatata tattggtgtt ctgggctctt acatgagaag atctagttaa 18840ctgttgttac tgaacagcga agacaaatat ataatttaag ctccccaact gctagtgatt 18900ctgttaagag gtaatgttta aagtaaattt acaagagccc gtctagctca gtcggtagag 18960cgcaaggctc ttaaccttgt ggtcgtgggt tcgagcccca cggtgggcgc acaatttttt 19020gttttttgac attttttgtt tgcttagttg cagacggttt ttcccctgct aggagatttc 19080cgagagaaaa aaaaggcact acaggttaac caaaaccacc aacctttgga gcgtcgaggc 19140gacggggcat ttgcgtagtt gaagcttaca aagttgcata tgagatgagt gccggacatg 19200aagcggataa cgttttaaac tggcaacaat atctagctgt ttcaaattca ggcgtgggaa 19260gctacgccta cgcgccctgg acggcgtgta aagagccagc atcggcatca ttgtcaaacg 19320atcgacaagg ccaagaaatt ccaaatatat tattaataaa aaagaaggca ccaaattagt 19380ttttgttttt tagtatgtgt ggcggaggaa attttgagaa cgaacgtatc caaagaaggc 19440acaagacgat atagattgac gcggctagaa agttgcagca agacagtggg tacggtctta 19500tatatcctaa taaataaaaa ataaaactat agtgtgtcaa atgtcaacaa gaggaggagg 19560cagccaaatt agcagaggga gacaagtaga gcacgcctta ttagcttgct tatttatcgt 19620ggtggtgtac ttgttaatta ctggcacgca ttatcaacaa cgcagttctg gatgtgaatc 19680tagacaaaca tttgtctagg ttccgcacgt atagtttttt ttcttttttt ttgggggggg 19740gggggaacgg aagctgtaat aaacggtact aggaacgaaa gcaaccgccg cgcgcatgtt 19800tttgcaatag attacggtga ccttgatgca ccaccgcgtg ctataaaaac cagtgtcccc 19860gagtctactc atcaaccaat ccataactcg aaaccttttc ttgtgctctg ttctgtctgt 19920gtgtttccaa agcaagcgaa agaggtcgag gggatcagct tcaagtttgt acaaaaaagc 19980aggctccgcg gccgccccct tcaccatgac gatggctcgt cctggggcgg ctttgccgct 20040gctgctggtc gtggtcggcg cttgctgcgc gcgcctggcg gcggcagtgc acctctccgc 20100gctcggcagg acactcatcg tcgaggcgtc gccgaaggcc ggacaagtcc tgcacgccgg 20160cgaggacacg ataaccgtga catggcacct caacgcgtcg gcgtccagcg tcgggtacaa 20220ggcgctggag gtgaccctct gctacgcgcc ggcgagccag gaggaccgcg ggtggcgcaa 20280ggccaacgac gacttgagca aggacaaggc gtgccagttc aggatcgccc ggcatgcata 20340cgccggcggc caggggacgc tccggtacag ggtcgcccgc gacgtcccca ccgcgtccta 20400ccacgtgcgc gcctacgcgc tggacgcgtc cggggcgccg gtgggctacg gccagaccgc 20460gcccgcctac tacttccacg tcgcgggcgt ctcgggcgtc cacgcgtccc tccgggtcgc 20520cgccgccgtg ctctccgcgt tctccatcgc cgcgctcgcc ttctttgtcg tcgtcgagaa 20580gaggaggaag gacgagtaga agggtgggcg cgccgaccca gctttcttgt acaaagtggc 20640cgttaacgga tccagacttg tccatcttct ggattggcca acttaattaa tgtatgaaat 20700aaaaggatgc acacatagtg acatgctaat cactataatg tgggcatcaa agttgtgtgt 20760tatgtgtaat tactagttat ctgaataaaa gagaaagaga tcatccatat ttcttatcct 20820aaatgaatgt cacgtgtctt tataattctt tgatgaacca gatgcatttc attaaccaaa 20880tccatataca tataaatatt aatcatatat aattaatatc aattgggtta gcaaaacaaa 20940tctagtctag gtgtgttttg cgaattgcgg caagcttgcg gccgccccgg gcaactttat 21000tatacaaagt tgatagatat cggaccgatt aaactttaat tcggtccgaa gcttgcatgc 21060ctgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta 21120agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta 21180tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa 21240tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga 21300gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt 21360ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg 21420gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt 21480agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata 21540taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa 21600aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga 21660cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga 21720cggcacggca tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg 21780acttgctccg ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac 21840ggcaggcggc ctcctcctcc tctcacggca ccggcagcta cgggggattc ctttcccacc 21900gctccttcgc tttcccttcc tcgcccgccg taataaatag acaccccctc cacaccctct 21960ttccccaacc tcgtgttgtt cggagcgcac acacacacaa ccagatctcc cccaaatcca 22020cccgtcggca cctccgcttc aaggtacgcc gctcgtcctc cccccccccc ctctctacct 22080tctctagatc ggcgttccgg tccatgcatg gttagggccc ggtagttcta cttctgttca 22140tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc 22200gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc 22260tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg 22320catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg 22380gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg 22440ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg gatttattaa ttttggatct 22500gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg gaaatatcga 22560tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga gatgcttttt 22620gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga 22680gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt atgtgtgtgt 22740catacatctt catagttacg agtttaagat ggatggaaat atcgatctag gataggtata 22800catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca tctattcata 22860tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata attattttga 22920tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt ttagccctgc 22980cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac cctgttgttt 23040ggtgttactt ctgcaggtcg actttaactt agcctaggat ccacacgaca ccatgtcccc 23100cgagcgccgc cccgtcgaga tccgcccggc caccgccgcc gacatggccg ccgtgtgcga 23160catcgtgaac cactacatcg agacctccac cgtgaacttc cgcaccgagc cgcagacccc 23220gcaggagtgg atcgacgacc tggagcgcct ccaggaccgc tacccgtggc tcgtggccga 23280ggtggagggc gtggtggccg gcatcgccta cgccggcccg tggaaggccc gcaacgccta 23340cgactggacc gtggagtcca ccgtgtacgt gtcccaccgc caccagcgcc tcggcctcgg 23400ctccaccctc tacacccacc tcctcaagag catggaggcc cagggcttca agtccgtggt 23460ggccgtgatc ggcctcccga acgacccgtc cgtgcgcctc cacgaggccc tcggctacac 23520cgcccgcggc accctccgcg ccgccggcta caagcacggc ggctggcacg acgtcggctt 23580ctggcagcgc gacttcgagc tgccggcccc gccgcgcccg gtgcgcccgg tgacgcagat 23640ctgagtcgaa acctagactt gtccatcttc tggattggcc aacttaatta atgtatgaaa 23700taaaaggatg cacacatagt gacatgctaa tcactataat gtgggcatca aagttgtgtg 23760ttatgtgtaa ttactagtta tctgaataaa agagaaagag atcatccata tttcttatcc 23820taaatgaatg tcacgtgtct ttataattct ttgatgaacc agatgcattt cattaaccaa 23880atccatatac atataaatat taatcatata taattaatat caattgggtt agcaaaacaa 23940atctagtcta ggtgtgtttt gcgaatgcgg ccgccaccgc ggtggagctc gaattcattc 24000cgattaatcg tggcctcttg ctcttcagga tgaagagcta tgtttaaacg tgcaagcgct 24060actagacaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca 24120atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc 24180tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga 24240gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa 24300ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg 24360atgacagagc gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc 24420agccttctta ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg 24480acataatagg aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag 24540aagtgaacgt tgacgatcgt cgaccgtacc ccgatgaatt aattcggacg tacgttctga 24600acacagctgg atacttactt gggcgattgt catacatgac atcaacaatg tacccgtttg 24660tgtaaccgtc tcttggaggt tcgtatgaca ctagtggttc ccctcagctt gcgactagat 24720gttgaggcct aacattttat tagagagcag gctagttgct tagatacatg atcttcaggc 24780cgttatctgt cagggcaagc gaaaattggc catttatgac gaccaatgcc ccgcagaagc 24840tcccatcttt gccgccatag acgccgcgcc ccccttttgg ggtgtagaac atccttttgc 24900cagatgtgga aaagaagttc gttgtcccat tgttggcaat gacgtagtag ccggcgaaag 24960tgcgagaccc atttgcgcta tatataagcc tacgatttcc gttgcgacta ttgtcgtaat 25020tggatgaact attatcgtag ttgctctcag agttgtcgta atttgatgga ctattgtcgt 25080aattgcttat ggagttgtcg tagttgcttg gagaaatgtc gtagttggat ggggagtagt 25140catagggaag acgagcttca tccactaaaa caattggcag gtcagcaagt gcctgccccg 25200atgccatcgc aagtacgagg cttagaacca ccttcaacag atcgcgcata gtcttcccca 25260gctctctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta 25320gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca 25380actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa 25440gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct 25500tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat 25560ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg 25620cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca 25680aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg 25740ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc 25800gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag 25860cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag 25920ctcgccgcgt tgtttcatca agccttacag tcaccgtaac cagcaaatca atatcactgt 25980gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt 26040cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca 26100ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtcggctt 26160gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt 26220cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc 26280cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct aatcgtatgc 26340caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg tgcgcgactc 26400ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga tcgttccatg 26460ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca tagcaagcag 26520agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc acacttctgg 26580tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga acaatgaaat 26640ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatc ttcatatgac 26700gcctaacgcc tggcacagcg gatcgcaaac ctggcgcggc ttttggcaca aaaggcgtga 26760caggtttgcg aatccgttgc tgccacttgt taaccctttt gccagatttg gtaactataa 26820tttatgttag aggcgaagtc ttgggtaaaa actggcctaa aattgctggg gatttcagga 26880aagtaaacat caccttccgg ctcgatgtct attgtagata tatgtagtgt atctacttga 26940tcgggggatc tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 27000gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 27060gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 27120tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 27180catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 27240tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 27300gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 27360atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 27420ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 27480cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 27540tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 27600gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 27660aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 27720tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 27780aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 27840aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 27900ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 27960ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 28020atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 28080atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 28140tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 28200gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 28260tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 28320gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 28380cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 28440gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggg 28500gggggggggg ggggggactt ccattgttca ttccacggac aaaaacagag aaaggaaacg 28560acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc ctttcttttc agagggtatt 28620ttaaataaaa

acattaagtt atgacgaaga agaacggaaa cgccttaaac cggaaaattt 28680tcataaatag cgaaaacccg cgaggtcgcc gccccgtaag ccgccccgta acctgtcgga 28740tcaccggaaa ggacccgtaa agtgataatg attatcatct acatatcaca acgtgcgtgg 28800aggccatcaa accacgtcaa ataatcaatt atgacgcagg tatcgtatta attgatctgc 28860atcaacttaa cgtaaaaaca acttcagaca atacaaatca gcgacactga atacggggca 28920acctcatgtc cccccccccc ccccccctgc aggcatcgtg gtgtcacgct cgtcgtttgg 28980tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 29040gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 29100agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 29160aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 29220gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac 29280tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 29340gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 29400tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 29460aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 29520catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 29580acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 29640tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga 29700attcggagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg 29760ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa 29820tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt 29880cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc 29940agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact 30000ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc 30060tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca 30120aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct 30180cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc 30240acgaggcaga cctcagcgcc agaaggccgc cagagaggcc gagcgcggcc gtgaggcttg 30300gacgctaggg cagggcatga aaaagcccgt agcgggctgc tacgggcgtc tgacgcggtg 30360gaaaggggga ggggatgttg tctacatggc tctgctgtag tgagtgggtt gcgctccggc 30420agcggtcctg atcaatcgtc accctttctc ggtccttcaa cgttcctgac aacgagcctc 30480cttttcgcca atccatcgac aatcaccgcg agtccctgct cgaacgctgc gtccggaccg 30540gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg agagcggagc ctgttcaacg 30600gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct cctcaagcac ggccccaaca 30660gtgaagtagc tgattgtcat cagcgcattg acggcgtccc cggccgaaaa acccgcctcg 30720cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg gtgcgcccgg tcgcgtgccg 30780gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct gcctgaagct gcgggcattc 30840ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca ccgaatgcgt atgattctcc 30900gccagcatgg cttcggccag tgcgtcgagc agcgcccgct tgttcctgaa gtgccagtaa 30960agcgccggct gctgaacccc caaccgttcc gccagtttgc gtgtcgtcag accgtctacg 31020ccgacctcgt tcaacaggtc cagggcggca cggatcactg tattcggctg caactttgtc 31080atgcttgaca ctttatcact gataaacata atatgtccac caacttatca gtgataaaga 31140atccgcgcgt tcaatcggac cagcggaggc tggtccggag gccagacgtg aaacccaaca 31200tacccctgat cgtaattctg agcactgtcg cgctcgacgc tgtcggcatc ggcctgatta 31260tgccggtgct gccgggcctc ctgcgcgatc tggttcactc gaacgacgtc accgcccact 31320atggcattct gctggcgctg tatgcgttgg tgcaatttgc ctgcgcacct gtgctgggcg 31380cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt ctcgctggcc ggcgccactg 31440tcgactacgc catcatggcg acagcgcctt tcctttgggt tctctatatc gggcggatcg 31500tggccggcat caccggggcg actggggcgg tagccggcgc ttatattgcc gatatcactg 31560atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc ctgtttcggg ttcgggatgg 31620tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc cccccacgct ccgttcttcg 31680ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg tttccttttg ccggagtcgc 31740acaaaggcga acgccggccg ttacgccggg aggctctcaa cccgctcgct tcgttccggt 31800gggcccgggg catgaccgtc gtcgccgccc tgatggcggt cttcttcatc atgcaacttg 31860tcggacaggt gccggccgcg ctttgggtca ttttcggcga ggatcgcttt cactgggacg 31920cgaccacgat cggcatttcg cttgccgcat ttggcattct gcattcactc gcccaggcaa 31980tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg ggcactcatg ctcggaatga 32040ttgccgacgg cacaggctac atcctgcttg ccttcgcgac acggggatgg atggcgttcc 32100cgatcatggt cctgcttgct tcgggtggca tcggaatgcc ggcgctgcaa gcaatgttgt 32160ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg ctcactggcg gcgctcacca 32220gcctgacctc gatcgtcgga cccctcctct tcacggcgat ctatgcggct tctataacaa 32280cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta cttgctctgc ctgccggcgc 32340tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga tcgctgatcg tggaaacgat 32400aggcctatgc catgcgggtc aaggcgactt ccggcaagct atacgcgccc taggagtgcg 32460gttggaacgt tggcccagcc agatactccc gatcacgagc aggacgccga tgatttgaag 32520cgcactcagc gtctgatcca agaacaacca tcctagcaac acggcggtcc ccgggctgag 32580aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa 32640gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt 32700aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc 32760cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag 32820cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc 32880tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc 32940caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat 33000cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat 33060aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca 33120ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc 33180ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga 33240cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat 33300gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc 33360ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt 33420cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat 33480ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc 33540gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc 33600tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc 33660tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct 33720cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc 33780gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg 33840aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat 33900ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa 33960cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc 34020aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat 34080tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg 34140tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc 34200tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg 34260tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg 34320cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc 34380cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc 34440agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt 34500cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc 34560gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg 34620ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg 34680acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca 34740tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc 34800cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat 34860tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag 34920tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc 34980acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa 35040cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccactct 35100tcattaaccg ctatatcgaa aattgcttgc ggcttgttag aattgccatg acgtacctcg 35160gtgtcacggg taagattacc gataaactgg aactgattat ggctcatatc gaaagtctcc 35220ttgagaaagg agactctagt ttagctaaac attggttccg ctgtcaagaa ctttagcggc 35280taaaattttg cgggccgcga ccaaaggtgc gaggggcggc ttccgctgtg tacaaccaga 35340tatttttcac caacatcctt cgtctgctcg atgagcgggg catgacgaaa catgagctgt 35400cggagagggc aggggtttca atttcgtttt tatcagactt aaccaacggt aaggccaacc 35460cctcgttgaa ggtgatggag gccattgccg acgccctgga aactccccta cctcttctcc 35520tggagtccac cgaccttgac cgcgaggcac tcgcggagat tgcgggtcat cctttcaaga 35580gcagcgtgcc gcccggatac gaacgcatca gtgtggtttt gccgtcacat aaggcgttta 35640tcgtaaagaa atggggcgac gacacccgaa aaaagctgcg tggaaggctc tgacgccaag 35700ggttagggct tgcacttcct tctttagccg ctaaaacggc cccttctctg cgggccgtcg 35760gctcgcgcat catatcgaca tcctcaacgg aagccgtgcc gcgaatggca tcgggcgggt 35820gcgctttgac agttgttttc tatcagaacc cctacgtcgt gcggttcgat tagctgtttg 35880tcttgcaggc taaacacttt cggtatatcg tttgcctgtg cgataatgtt gctaatgatt 35940tgttgcgtag gggttactga aaagtgagcg ggaaagaaga gtttcagacc atcaaggagc 36000gggccaagcg caagctggaa cgcgacatgg gtgcggacct gttggccgcg ctcaacgacc 36060cgaaaaccgt tgaagtcatg ctcaacgcgg acggcaaggt gtggcacgaa cgccttggcg 36120agccgatgcg gtacatctgc gacatgcggc ccagccagtc gcaggcgatt atagaaacgg 36180tggccggatt ccacggcaaa gaggtcacgc ggcattcgcc catcctggaa ggcgagttcc 36240ccttggatgg cagccgcttt gccggccaat tgccgccggt cgtggccgcg ccaacctttg 36300cgatccgcaa gcgcgcggtc gccatcttca cgctggaaca gtacgtcgag gcgggcatca 36360tgacccgcga gcaatacgag gtcattaaaa gcgccgtcgc ggcgcatcga aacatcctcg 36420tcattggcgg tactggctcg ggcaagacca cgctcgtcaa cgcgatcatc aatgaaatgg 36480tcgccttcaa cccgtctgag cgcgtcgtca tcatcgagga caccggcgaa atccagtgcg 36540ccgcagagaa cgccgtccaa taccacacca gcatcgacgt ctcgatgacg ctgctgctca 36600agacaacgct gcgtatgcgc cccgaccgca tcctggtcgg tgaggtacgt ggccccgaag 36660cccttgatct gttgatggcc tggaacaccg ggcatgaagg aggtgccgcc accctgcacg 36720caaacaaccc caaagcgggc ctgagccggc tcgccatgct tatcagcatg cacccggatt 36780caccgaaacc cattgagccg ctgattggcg aggcggttca tgtggtcgtc catatcgcca 36840ggacccctag cggccgtcga gtgcaagaaa ttctcgaagt tcttggttac gagaacggcc 36900agtacatcac caaaaccctg taaggagtat ttccaatgac aacggctgtt ccgttccgtc 36960tgaccatgaa tcgcggcatt ttgttctacc ttgccgtgtt cttcgttctc gctctcgcgt 37020tatccgcgca tccggcgatg gcctcggaag gcaccggcgg cagcttgcca tatgagagct 37080ggctgacgaa cctgcgcaac tccgtaaccg gcccggtggc cttcgcgctg tccatcatcg 37140gcatcgtcgt cgccggcggc gtgctgatct tcggcggcga actcaacgcc ttcttccgaa 37200ccctgatctt cctggttctg gtgatggcgc tgctggtcgg cgcgcagaac gtgatgagca 37260ccttcttcgg tcgtggtgcc gaaatcgcgg ccctcggcaa cggggcgctg caccaggtgc 37320aagtcgcggc ggcggatgcc gtgcgtgcgg tagcggctgg acggctcgcc taatcatggc 37380tctgcgcacg atccccatcc gtcgcgcagg caaccgagaa aacctgttca tgggtggtga 37440tcgtgaactg gtgatgttct cgggcctgat ggcgtttgcg ctgattttca gcgcccaaga 37500gctgcgggcc accgtggtcg gtctgatcct gtggttcggg gcgctctatg cgttccgaat 37560catggcgaag gccgatccga agatgcggtt cgtgtacctg cgtcaccgcc ggtacaagcc 37620gtattacccg gcccgctcga ccccgttccg cgagaacacc aatagccaag ggaagcaata 37680ccgatgatcc aagcaattgc gattgcaatc gcgggcctcg gcgcgcttct gttgttcatc 37740ctctttgccc gcatccgcgc ggtcgatgcc gaactgaaac tgaaaaagca tcgttccaag 37800gacgccggcc tggccgatct gctcaactac gccgctgtcg tcgatgacgg cgtaatcgtg 37860ggcaagaacg gcagctttat ggctgcctgg ctgtacaagg gcgatgacaa cgcaagcagc 37920accgaccagc agcgcgaagt agtgtccgcc cgcatcaacc aggccctcgc gggcctggga 37980agtgggtgga tgatccatgt ggacgccgtg cggcgtcctg ctccgaacta cgcggagcgg 38040ggcctgtcgg cgttccctga ccgtctgacg gcagcgattg aagaagagcg ctcggtcttg 38100ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc 38160atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca 38220tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc 38280ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc 38340gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc 38400cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc 38460cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg 38520ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc 38580atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc 38640aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct 38700cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa 38760gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga 38820aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgct gcttccctgc 38880tgttttgtgg aatatctacc gactggaaac aggcaaatgc aggaaattac tgaactgagg 38940ggacaggcga gagacgatgc caaagagcta caccgacgag ctggccgagt gggttgaatc 39000ccgcgcggcc aagaagcgcc ggcgtgatga ggctgcggtt gcgttcctgg cggtgagggc 39060ggatgtcgag gcggcgttag cgtccggcta tgcgctcgtc accatttggg agcacatgcg 39120ggaaacgggg aaggtcaagt tctcctacga gacgttccgc tcgcacgcca ggcggcacat 39180caaggccaag cccgccgatg tgcccgcacc gcaggccaag gctgcggaac ccgcgccggc 39240acccaagacg ccggagccac ggcggccgaa gcaggggggc aaggctgaaa agccggcccc 39300cgctgcggcc ccgaccggct tcaccttcaa cccaacaccg gacaaaaagg atctactgta 39360atggcgaaaa ttcacatggt tttgcagggc aagggcgggg tcggcaagtc ggccatcgcc 39420gcgatcattg cgcagtacaa gatggacaag gggcagacac ccttgtgcat cgacaccgac 39480ccggtgaacg cgacgttcga gggctacaag gccctgaacg tccgccggct gaacatcatg 39540gccggcgacg aaattaactc gcgcaacttc gacaccctgg tcgagctgat tgcgccgacc 39600aaggatgacg tggtgatcga caacggtgcc agctcgttcg tgcctctgtc gcattacctc 39660atcagcaacc aggtgccggc tctgctgcaa gaaatggggc atgagctggt catccatacc 39720gtcgtcaccg gcggccaggc tctcctggac acggtgagcg gcttcgccca gctcgccagc 39780cagttcccgg ccgaagcgct tttcgtggtc tggctgaacc cgtattgggg gcctatcgag 39840catgagggca agagctttga gcagatgaag gcgtacacgg ccaacaaggc ccgcgtgtcg 39900tccatcatcc agattccggc cctcaaggaa gaaacctacg gccgcgattt cagcgacatg 39960ctgcaagagc ggctgacgtt cgaccaggcg ctggccgatg aatcgctcac gatcatgacg 40020cggcaacgcc tcaagatcgt gcggcgcggc ctgtttgaac agctcgacgc ggcggccgtg 40080ctatgagcga ccagattgaa gagctgatcc gggagattgc ggccaagcac ggcatcgccg 40140tcggccgcga cgacccggtg ctgatcctgc ataccatcaa cgcccggctc atggccgaca 40200gtgcggccaa gcaagaggaa atccttgccg cgttcaagga agagctggaa gggatcgccc 40260atcgttgggg cgaggacgcc aaggccaaag cggagcggat gctgaacgcg gccctggcgg 40320ccagcaagga cgcaatggcg aaggtaatga aggacagcgc cgcgcaggcg gccgaagcga 40380tccgcaggga aatcgacgac ggccttggcc gccagctcgc ggccaaggtc gcggacgcgc 40440ggcgcgtggc gatgatgaac atgatcgccg gcggcatggt gttgttcgcg gccgccctgg 40500tggtgtgggc ctcgttatga atcgcagagg cgcagatgaa aaagcccggc gttgccgggc 40560tttgtttttg cgttagctgg gcttgtttga caggcccaag ctctgactgc gcccgcgctc 40620gcgctcctgg gcctgtttct tctcctgctc ctgcttgcgc atcagggcct ggtgccgtcg 40680ggctgcttca cgcatcgaat cccagtcgcc ggccagctcg ggatgctccg cgcgcatctt 40740gcgcgtcgcc agttcctcga tcttgggcgc gtgaatgccc atgccttcct tgatttcgcg 40800caccatgtcc agccgcgtgt gcagggtctg caagcgggct tgctgttggg cctgctgctg 40860ctgccaggcg gcctttgtac gcggcaggga cagcaagccg ggggcattgg actgtagctg 40920ctgcaaacgc gcctgctgac ggtctacgag ctgttctagg cggtcctcga tgcgctccac 40980ctggtcatgc tttgcctgca cgtagagcgc aagggtctgc tggtaggtct gctcgatggg 41040cgcggattct aagagggcct gctgttccgt ctcggcctcc tgggccgcct gtagcaaatc 41100ctcgccgctg ttgccgctgg actgctttac tgccggggac tgctgttgcc ctgctcgcgc 41160cgtcgtcgca gttcggcttg cccccactcg attgactgct tcatttcgag ccgcagcgat 41220gcgatctcgg attgcgtcaa cggacggggc agcgcggagg tgtccggctt ctccttgggt 41280gagtcggtcg atgccatagc caaaggtttc cttccaaaat gcgtccattg ctggaccgtg 41340tttctcattg atgcccgcaa gcatcttcgg cttgaccgcc aggtcaagcg cgccttcatg 41400ggcggtcatg acggacgccg ccatgacctt gccgccgttg ttctcgatgt agccgcgtaa 41460tgaggcaatg gtgccgccca tcgtcagcgt gtcatcgaca acgatgtact tctggccggg 41520gatcacctcc ccctcgaaag tcgggttgaa cgccaggcga tgatctgaac cggctccggt 41580tcgggcgacc ttctcccgct gcacaatgtc cgtttcgacc tcaaggccaa ggcggtcggc 41640cagaacgacc gccatcatgg ccggaatctt gttgttcccc gccgcctcga cggcgaggac 41700tggaacgatg cggggcttgt cgtcgccgat cagcgtcttg agctgggcaa cagtgtcgtc 41760cgaaatcagg cgctcgacca aattaagcgc cgcttccgcg tcgccctgct tcgcagcctg 41820gtattcaggc tcgttggtca aagaaccaag gtcgccgttg cgaaccacct tcgggaagtc 41880tccccacggt gcgcgctcgg ctctgctgta gctgctcaag acgcctccct ttttagccgc 41940taaaactcta acgagtgcgc ccgcgactca acttgacgct ttcggcactt acctgtgcct 42000tgccacttgc gtcataggtg atgcttttcg cactcccgat ttcaggtact ttatcgaaat 42060ctgaccgggc gtgcattaca aagttcttcc ccacctgttg gtaaatgctg ccgctatctg 42120cgtggacgat gctgccgtcg tggcgctgcg acttatcggc cttttgggcc atatagatgt 42180tgtaaatgcc aggtttcagg gccccggctt tatctacctt ctggttcgtc catgcgcctt 42240ggttctcggt ctggacaatt ctttgcccat tcatgaccag gaggcggtgt ttcattgggt 42300gactcctgac ggttgcctct ggtgttaaac gtgtcctggt cgcttgccgg ctaaaaaaaa 42360gccgacctcg gcagttcgag gccggctttc cctagagccg ggcgcgtcaa ggttgttcca 42420tctattttag tgaactgcgt tcgatttatc agttactttc ctcccgcttt gtgtttcctc 42480ccactcgttt ccgcgtctag ccgacccctc aacatagcgg cctcttcttg ggctgccttt 42540gcctcttgcc gcgcttcgtc acgctcggct tgcaccgtcg taaagcgctc ggcctgcctg 42600gccgcctctt gcgccgccaa cttcctttgc tcctggtggg cctcggcgtc ggcctgcgcc 42660ttcgctttca ccgctgccaa ctccgtgcgc aaactctccg cttcgcgcct ggtggcgtcg 42720cgctcgccgc gaagcgcctg catttcctgg ttggccgcgt ccagggtctt gcggctctct 42780tctttgaatg cgcgggcgtc ctggtgagcg tagtccagct cggcgcgcag ctcctgcgct 42840cgacgctcca cctcgtcggc ccgctgcgtc gccagcgcgg cccgctgctc ggctcctgcc 42900agggcggtgc gtgcttcggc cagggcttgc cgctggcgtg cggccagctc ggccgcctcg 42960gcggcctgct gctctagcaa tgtaacgcgc gcctgggctt cttccagctc gcgggcctgc 43020gcctcgaagg cgtcggccag ctccccgcgc acggcttcca actcgttgcg ctcacgatcc 43080cagccggctt gcgctgcctg caacgattca ttggcaaggg cctgggcggc ttgccagagg 43140gcggccacgg cctggttgcc ggcctgctgc accgcgtccg gcacctggac tgccagcggg 43200gcggcctgcg ccgtgcgctg gcgtcgccat tcgcgcatgc cggcgctggc gtcgttcatg 43260ttgacgcggg cggccttacg cactgcatcc acggtcggga agttctcccg gtcgccttgc 43320tcgaacagct cgtccgcagc cgcaaaaatg cggtcgcgcg tctctttgtt cagttccatg 43380ttggctccgg taattggtaa gaataataat actcttacct accttatcag cgcaagagtt 43440tagctgaaca gttctcgact taacggcagg ttttttagcg gctgaagggc aggcaaaaaa 43500agccccgcac ggtcggcggg ggcaaagggt cagcgggaag gggattagcg ggcgtcgggc 43560ttcttcatgc gtcggggccg cgcttcttgg gatggagcac gacgaagcgc gcacgcgcat 43620cgtcctcggc cctatcggcc cgcgtcgcgg tcaggaactt gtcgcgcgct aggtcctccc 43680tggtgggcac

caggggcatg aactcggcct gctcgatgta ggtccactcc atgaccgcat 43740cgcagtcgag gccgcgttcc ttcaccgtct cttgcaggtc gcggtacgcc cgctcgttga 43800gcggctggta acgggccaat tggtcgtaaa tggctgtcgg ccatgagcgg cctttcctgt 43860tgagccagca gccgacgacg aagccggcaa tgcaggcccc tggcacaacc aggccgacgc 43920cgggggcagg ggatggcagc agctcgccaa ccaggaaccc cgccgcgatg atgccgatgc 43980cggtcaacca gcccttgaaa ctatccggcc ccgaaacacc cctgcgcatt gcctggatgc 44040tgcgccggat agcttgcaac atcaggagcc gtttcttttg ttcgtcagtc atggtccgcc 44100ctcaccagtt gttcgtatcg gtgtcggacg aactgaaatc gcaagagctg ccggtatcgg 44160tccagccgct gtccgtgtcg ctgctgccga agcacggcga ggggtccgcg aacgccgcag 44220acggcgtatc cggccgcagc gcatcgccca gcatggcccc ggtcagcgag ccgccggcca 44280ggtagcccag catggtgctg ttggtcgccc cggccaccag ggccgacgtg acgaaatcgc 44340cgtcattccc tctggattgt tcgctgctcg gcggggcagt gcgccgcgcc ggcggcgtcg 44400tggatggctc gggttggctg gcctgcgacg gccggcgaaa ggtgcgcagc agctcgttat 44460cgaccggctg cggcgtcggg gccgccgcct tgcgctgcgg tcggtgttcc ttcttcggct 44520cgcgcagctt gaacagcatg atcgcggaaa ccagcagcaa cgccgcgcct acgcctcccg 44580cgatgtagaa cagcatcgga ttcattcttc ggtcctcctt gtagcggaac cgttgtctgt 44640gcggcgcggg tggcccgcgc cgctgtcttt ggggatcagc cctcgatgag cgcgaccagt 44700ttcacgtcgg caaggttcgc ctcgaactcc tggccgtcgt cctcgtactt caaccaggca 44760tagccttccg ccggcggccg acggttgagg ataaggcggg cagggcgctc gtcgtgctcg 44820acctggacga tggccttttt cagcttgtcc gggtccggct ccttcgcgcc cttttccttg 44880gcgtccttac cgtcctggtc gccgtcctcg ccgtcctggc cgtcgccggc ctccgcgtca 44940cgctcggcat cagtctggcc gttgaaggca tcgacggtgt tgggatcgcg gcccttctcg 45000tccaggaact cgcgcagcag cttgaccgtg ccgcgcgtga tttcctgggt gtcgtcgtca 45060agccacgcct cgacttcctc cgggcgcttc ttgaaggccg tcaccagctc gttcaccacg 45120gtcacgtcgc gcacgcggcc ggtgttgaac gcatcggcga tcttctccgg caggtccagc 45180agcgtgacgt gctgggtgat gaacgccggc gacttgccga tttccttggc gatatcgcct 45240ttcttcttgc ccttcgccag ctcgcggcca atgaagtcgg caatttcgcg cggggtcagc 45300tcgttgcgtt gcaggttctc gataacctgg tcggcttcgt tgtagtcgtt gtcgatgaac 45360gccgggatgg acttcttgcc ggcccacttc gagccacggt agcggcgggc gccgtgattg 45420atgatatagc ggcccggctg ctcctggttc tcgcgcaccg aaatgggtga cttcaccccg 45480cgctctttga tcgtggcacc gatttccgcg atgctctccg gggaaaagcc ggggttgtcg 45540gccgtccgcg gctgatgcgg atcttcgtcg atcaggtcca ggtccagctc gatagggccg 45600gaaccgccct gagacgccgc aggagcgtcc aggaggctcg acaggtcgcc gatgctatcc 45660aaccccaggc cggacggctg cgccgcgcct gcggcttcct gagcggccgc agcggtgttt 45720ttcttggtgg tcttggcttg agccgcagtc attgggaaat ctccatcttc gtgaacacgt 45780aatcagccag ggcgcgaacc tctttcgatg ccttgcgcgc ggccgttttc ttgatcttcc 45840agaccggcac accggatgcg agggcatcgg cgatgctgct gcgcaggcca acggtggccg 45900gaatcatcat cttggggtac gcggccagca gctcggcttg gtggcgcgcg tggcgcggat 45960tccgcgcatc gaccttgctg ggcaccatgc caaggaattg cagcttggcg ttcttctggc 46020gcacgttcgc aatggtcgtg accatcttct tgatgccctg gatgctgtac gcctcaagct 46080cgatggggga cagcacatag tcggccgcga agagggcggc cgccaggccg acgccaaggg 46140tcggggccgt gtcgatcagg cacacgtcga agccttggtt cgccagggcc ttgatgttcg 46200ccccgaacag ctcgcgggcg tcgtccagcg acagccgttc ggcgttcgcc agtaccgggt 46260tggactcgat gagggcgagg cgcgcggcct ggccgtcgcc ggctgcgggt gcggtttcgg 46320tccagccgcc ggcagggaca gcgccgaaca gcttgcttgc atgcaggccg gtagcaaagt 46380ccttgagcgt gtaggacgca ttgccctggg ggtccaggtc gatcacggca acccgcaagc 46440cgcgctcgaa aaagtcgaag gcaagatgca caagggtcga agtcttgccg acgccgcctt 46500tctggttggc cgtgaccaaa gttttcatcg tttggtttcc tgttttttct tggcgtccgc 46560ttcccacttc cggacgatgt acgcctgatg ttccggcaga accgccgtta cccgcgcgta 46620cccctcgggc aagttcttgt cctcgaacgc ggcccacacg cgatgcaccg cttgcgacac 46680tgcgcccctg gtcagtccca gcgacgttgc gaacgtcgcc tgtggcttcc catcgactaa 46740gacgccccgc gctatctcga tggtctgctg ccccacttcc agcccctgga tcgcctcctg 46800gaactggctt tcggtaagcc gtttcttcat ggataacacc cataatttgc tccgcgcctt 46860ggttgaacat agcggtgaca gccgccagca catgagagaa gtttagctaa acatttctcg 46920cacgtcaaca cctttagccg ctaaaactcg tccttggcgt aacaaaacaa aagcccggaa 46980accgggcttt cgtctcttgc cgcttatggc tctgcacccg gctccatcac caacaggtcg 47040cgcacgcgct tcactcggtt gcggatcgac actgccagcc caacaaagcc ggttgccgcc 47100gccgccagga tcgcgccgat gatgccggcc acaccggcca tcgcccacca ggtcgccgcc 47160ttccggttcc attcctgctg gtactgcttc gcaatgctgg acctcggctc accataggct 47220gaccgctcga tggcgtatgc cgcttctccc cttggcgtaa aacccagcgc cgcaggcggc 47280attgccatgc tgcccgccgc tttcccgacc acgacgcgcg caccaggctt gcggtccaga 47340ccttcggcca cggcgagctg cgcaaggaca taatcagccg ccgacttggc tccacgcgcc 47400tcgatcagct cttgcactcg cgcgaaatcc ttggcctcca cggccgccat gaatcgcgca 47460cgcggcgaag gctccgcagg gccggcgtcg tgatcgccgc cgagaatgcc cttcaccaag 47520ttcgacgaca cgaaaatcat gctgacggct atcaccatca tgcagacgga tcgcacgaac 47580ccgctgaatt gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa 47640aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc 47700cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca 47760cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg 47820ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg 47880cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt 47940cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg 48000ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg 48060ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca 48120aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc 48180gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc 48240aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc 48300gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc 48360gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc 48420aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac 48480ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc 48540agcccggtga gcgtcggaaa ggcgctggaa gccccgtagc gacgcggaga ggggcgagac 48600aagccaaggg cgcaggctcg atgcgcagca cgacatagcc ggttctcgca aggacgagaa 48660tttccctgcg gtgcccctca agtgtcaatg aaagtttcca acgcgagcca ttcgcgagag 48720ccttgagtcc acgctagatg agagctttgt tgtaggtgga ccagttggtg attttgaact 48780tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 48840cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 48900ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 48960atacaagggg tgttatgagc catattcaac gggaaac 48997

Claims

1. An isolated polynucleotide comprising: (a) a nucleotide sequence encoding a high affinity nitrate transporter polypeptide, wherein the polypeptide has an amino acid sequence of at least 90% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 92; or (b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.

2-3. (canceled)

4. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide has at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 92.

5. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide has at least 99% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 92.

6. The polynucleotide of claim 1, wherein the amino acid sequence of the polypeptide comprises one of SEQ ID NO: 92.

7. (canceled)

8. The isolated polynucleotide of claim 1, wherein the polypeptide sequence comprises at least two motifs selected from group consisting of SEQ ID NOs: 50, 51 and 52.

9. (canceled)

10. A recombinant DNA construct comprising ai the isolated polynucleotide of claim 1, the polynucleotide operably linked to at least one regulatory sequence.

11. (canceled)

12. A plant comprising in its genome the recombinant DNA construct of claim 10.

13. A transgenic seed obtained from the plant of claim 12.

14. The plant of claim 12, wherein said plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.

15. A plant cell comprising in its genome the recombinant DNA construct of claim 10.

16. Plant tissue comprising the plant cell of claim 15.

17-34. (canceled)

35. A method of increasing nitrogen transport in a plant, the method comprising expressing a recombinant nucleotide sequence encoding a polypeptide comprising a sequence of at least 90% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NOs: 92; and growing the plant.

36. The method of claim 35, wherein the polypeptide sequence comprises at least two motifs selected from group consisting of SEQ ID NOs: 50, 51 and 52.

37. The method of claim 35, further comprising expressing at least one additional recombinant DNA construct comprising an isolated polynucleotide encoding a NAR polypeptide, operably linked to at least one regulatory sequence.

38. The method of claim 35, wherein the plant is selected from the group consisting of rice, corn, sorghum, millet, rye, soybean, canola, wheat, barley, oat, beans, and nuts.

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