NOVEL PRRS VIRUS INDUCING TYPE I INTERFERON IN SUSCEPTIBLE CELLS

Abstract

The present invention relates to the field of attenuated live viruses useful as vaccine or medicament for preventing or treating Porcine Reproductive and Respiratory Syndrome (PRRS) in swine, and is based on the surprising finding of a PRRS virus which is able to induce the interferon type I response of a cell infected by said virus. In one embodiment, the PRRS virus according to the invention is a PRRS virus mutant comprising, in comparison with the genome of a wild type strain, a mutation in the gene encoding the non structural protein 1 (nsp1) of said virus.

Description

SEQUENCE LISTING

[0001] The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jul. 29, 2011 is named 01-2738-SEQ.txt and is 236,320 bytes in size.

FIELD OF THE INVENTION

[0002] The present invention belongs to the field of vaccines and medicaments for the prophylaxis and treatment of infectious diseases. In particular, it relates to attenuated live viruses useful as vaccine or medicament for preventing or treating Porcine Reproductive and Respiratory Syndrome (PRRS), a viral disease affecting swine.

BACKGROUND OF THE INVENTION

[0003] Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the virus family Arteriviridae and belongs, together with the Coronaviridae, to the virus order Nidovirales. PRRSV is an enveloped virus with a single-stranded, positive-sense RNA genome of about 15 kilobases comprising nine open reading frames (ORFs), namely ORF1a, ORF1ab, ORF2a, ORF 2ab, and ORFs 3 through ORF7. ORFs 1a and 1ab encode large polyproteins that are processed into the viral nonstructural proteins (nsp) by auto- and transcleavages of viral proteases nsp1, nsp2, and nsp4 (Snijder and Meulenberg, 1998).

[0004] There are two distinct viral PRRSV genotypes causing similar clinical symptoms that diverge by about 40% on nucleotide sequence level, genotype I (EU) and genotype II (US). The North American (US) prototype strain is VR-2332, while the European (EU) prototype strain is Lelystad virus.

[0005] PRRSV is considered one of the economically most important infectious agents in pigs causing late-term reproductive failure in sows and respiratory disease in growing pigs. Often, PRRSV infection is complicated by secondary bacterial infections being attributed to the immunosuppressive nature of the virus. Also, PRRSV viremia lasts for weeks, and virus then still can be detected in lymphoid organs for several months, demonstrating difficulties or failure of the host's immune response to clear the virus (Allende et al., 2000).

[0006] The specific immune response to PRRSV infection is characterized by delayed induction of neutralizing antibodies (Lopez and Osorio, 2004) and short cell-mediated immune response (Xiao et al., 2004). It is commonly accepted that these effects can in part be attributed, along with presentation of decoy epitopes (Ostrowski et al., 2002; Ansari et al., 2006) and glycan shielding of viral envelope proteins (Ansari et al., 2006), to the viral inhibition of the host's innate immune system. It has been demonstrated that PRRSV infection does not or only weakly or delayedly induce production of type I interferon (IFN) (interferon α and interferon β; (Miller et al., 2004)) or type II IFN, (interferon γ; (Meier et al., 2003)) in susceptible cell lines (swine pulmonary alveolar macrophages, monkey kidney cells MARC-145) and/or pigs (Buddaert et al., 1998).

[0007] Thus, there is a need for novel vaccines and medications effecting a rapid induction of neutralizing antibodies and interferon responses for the prophylaxis and treatment of PRRSV infection.

BRIEF DESCRIPTION OF THE FIGURES

[0008] FIG. 1: Sequence alignment of nsp1β protein sequences from parental EU PRRSV strain LoN94-13 and from vaccine candidates.

[0009] FIG. 2: Sequence alignment of nsp1β proteins from PRRSV strains.

[0010] FIG. 3: PRRSV-specific immunofluorescence of virus stock titrations. (A) delta nsp1 XVII-1; (B) delta nsp1 XVIII-12. Black, negative fluorescence; light grey, few positive cells; grey, foci of positive cells; dark grey, complete cell monolayer positive.

[0011] FIG. 4: Virus stocks of vaccine candidates contain IFNβ.

[0012] FIG. 5: Time course of IFNβ induction after infection of MA104 cells.

[0013] FIG. 6: Delta nsp1 mutants show increased sensitivity to type I IFN.

[0014] FIG. 7: Mean body temperatures of vaccinated groups before and after challenge.

DETAILED DESCRIPTION

[0015] IFNs play an important role in establishing an effective adaptive immune response against viral infections, and many viruses therefore have developed strategies and counteractions against onset of the host's innate immune system (Haller and Weber, 2009). In the interest to identify the anticipated PRRSV IFN antagonist(s), extensive screening analyses based on cell lines stably expressing genes of interest or on cells transfected with protein-expressing plasmids have identified several PRRSV nonstructural proteins (nsps) including nsp1 (see below), nsp2 (Beura et al., 2010; Li et al., 2010), nsp4 (Beura et al., 2010), and nsp11 (Beura et al., 2010; Shi et al., 2011a) to be involved in blocking the induction of type I IFN.

[0016] nsp1 is located at the N-terminus of the PRRSV ORF1a-derived polyprotein 1a and is processed into two multifunctional subunits, nsp1α and nsp1β, each of which contains a papain-like cystein protease (PCP) domain essential for self-release from the viral polyprotein (den Boon et al., 1995; Chen et al., 2010). nsp1α contains an N-terminal zinc-finger domain and the PCPα protease domain, while nsp1β contains PCPβ. For both nsp1 subunits, nsp1α and nsp1β, the three-dimensional crystal structure has been resolved (Sun et al., 2009; Xue et al., 2010). According to these analyses, nsp1β consists of an N-terminal domain (NTD), a linker domain (LKD), the PCP domain (PCP beta), and a C-terminal extension (CTE); (Xue et al., 2010), see FIG. 2. C-terminal, nsp1β-mediated cleavage of nsp1 from nsp2 occurs at site WYG/AGR for PRRSV US strains (Kroese et al., 2008) or is predicted at site WYG/AAG for PRRSV EU strains (Chen et al., 2010), while nsp1α/nsp1β cleavage occurs at site ECAM/A×VYD for PRRSV US strains or is predicted at site EEAH/S×VYR for PRRSV EU strains (Chen et al., 2010).

[0017] Several studies on protein level demonstrated to the mechanistic detail that PRRSV nsp1 and/or its autocleavage-derived subunits nsp1a and/or nsp1β inhibit type I IFN production by interfering with IFN transcription (Song et al., 2010; Kim et al., 2010; Chen et al., 2010; Beura et al., 2010). In addition, it has been demonstrated that nsp1β interferes with the cellular response to interferon (interferon signaling); (Chen et al., 2010). Moreover, it was demonstrated that PRRSV infection inhibits IFNα and/or IFNβ production in PRRSV infected cells in vitro (Kim et al., 2010; Beura et al., 2010), the subcellular localization of nsp1 (subunits) was determined (Song et al., 2010; Chen et al., 2010), and mechanistic aspects of type I IFN inhibition that were obtained by others from single protein expression experiments were confirmed in cells infected with PRRSV (Shi et al., 2010). Finally, a nsp1 mutagenesis study based on nsp1 protein expression investigated effects on viral IFN inhibition (Shi et al., 2011b), showing that mutations that inactivated papain-like cysteine protease activity of nsp1α made nsp1 lose its IFN antagonism activity, whereas mutations that inactivated papain-like cysteine protease activity of nsp1β did not influence the IFN antagonism activity of nsp1.

[0018] However, a viable PRRSV strain that induces IFN production and/or does not interfere with IFN signaling after infection of susceptible cells and/or the host, in particular a PRRSV strain comprising a genomic mutation in its nsp1 gene, has not been described yet.

[0019] It is thus an aim of the present invention to make available such a viable PRRS virus inducing the IFN response of a cell, in particular of a host cell, wherein said PRRS virus may serve as an effective vaccine or medicament for the prophylaxis or treatment of the Porcine reproductive and respiratory syndrome in swine.

[0020] The solution to the above technical problem is achieved by the description and the embodiments characterized in the claims.

[0021] Thus, the invention in its different aspects and embodiments is implemented according to the claims.

[0022] The invention is based on the surprising finding of a Porcine Reproductive and Respiratory Syndrome (PRRS) virus which is able to induce the interferon type I response of a cell, in particular of a host cell.

[0023] Hence, one aspect of the invention concerns a PRRS virus (PRRSV) which is able to induce the interferon type I production and secretion by a cell infected by said virus, wherein the PRRS virus according to the invention in particular is able to induce or induces the interferon type I production and secretion by an interferon competent cell infected by said virus.

[0024] As used herein, it is understood that the terms "interferon type I", "IFN type I", "type I interferon" and "type I IFN" are equivalent.

[0025] Interferon type I production and secretion by a cell, as described herein, particularly means that interferon type I is made and released by a cell in response to the infection of the PRRS virus according to the invention. In this regard, interferon type I, as mentioned herein, is preferably interferon-α and/or interferon-β.

[0026] The infection of a cell by the PRRS virus according to the invention in particular includes attachment of the virus to the cell, entry of the virus into the cell, disassembly of the virion, and preferably replication and transcription of the viral genome, expression of viral proteins, and assembly and release of new infectious viral particles.

[0027] The cell, as mentioned herein, is a primary or secondary susceptible cell, preferably a mammalian cell, in particular a porcine or a simian cell, more preferably said cell is a porcine macrophage or a MA-104 cell or a MARC-145 cell or a Vero cell.

[0028] It is further understood that the PRRS virus according to the invention is able to induce in vitro and/or in vivo the interferon type I production and secretion by a cell infected by said virus.

[0029] Preferably, the PRRS virus according to the invention is a live PRRS virus and/or a modified-live PRRS virus and/or an attenuated PRRS virus.

[0030] The term "attenuated PRRS virus", as described herein, is in particular directed to a PRRS virus which is attenuated in vitro and/or in vivo, more particular in susceptible cell lines and/or the host.

[0031] The term "host", as used herein, is in particular directed to animals infectable with PRRS virus, in particular swine, more particular pigs, such as domestic pigs.

[0032] As mentioned herein, "attenuated" particularly relates to a reduced virulence of a pathogen, in particular of a wild type PRRS virus, wherein "virulence" is understood to be the degree of pathogenicity, and wherein "pathogenicity" is directed to the ability of the pathogen to produce clinical symptoms in the host, such as elevated body temperature.

[0033] In particular preferably, the PRRS virus according to the invention has or shows increased sensitivity to type I INF when compared to wild type PRRSV, wherein the term "sensitivity to type I INF" is understood as reduced viral infectivity when IFNβ is present, preferably in a sufficient amount for significantly reducing viral infectivity of wild type PRRSV, in the medium surrounding the virus at the time of infection.

[0034] The term "wild type PRRS virus" or "wild type PRRSV", respectively, as used herein, is in particular directed to an infectious pathogenic PRRS virus, which is particularly capable of causing PRRS in swine. In one particular preferred embodiment, the term "wild type PRRS virus" is directed to a PRRS virus whose genome comprises a RNA sequence or consists of a RNA polynucleotide, wherein said RNA sequence or RNA polynucleotide is a RNA copy of a polynucleotide selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 14 and SEQ ID NO: 15.

[0035] In one embodiment, the PRRS virus according to the invention is a PRRS virus mutant, in particular comprising, in comparison with the genome of a wild type PRRSV strain, a mutation in a gene encoding a protein of said virus.

[0036] In a preferred embodiment, the PRRS virus according to the invention comprises a mutation in the gene encoding the nsp1 protein of said virus. Thus, the invention preferably concerns a PRRS virus which is able to induce the interferon type I production and secretion by an infected cell as a result of a mutation in the gene encoding the nsp1 protein, wherein said mutation is preferably a mutation as mentioned hereinafter.

[0037] Hence, the invention particularly concerns a PRRS virus comprising, in comparison with the genome of a wild type strain, a mutation in the gene encoding the nsp1 protein. Accordingly, the mutation as described herein is preferably a mutation in comparison with the sequence of the corresponding wild type gene encoding the nsp1 protein.

[0038] The invention is thus preferably directed to a PRRS virus; in particular a wild type PRRS virus, wherein a mutation, preferably a mutation as mentioned hereinafter, has been implemented in the genome of said virus resulting in a non-natural nsp1 protein or in the lack of nsp1 protein of said virus.

[0039] In the context of the invention it is understood, that the mutation as described herein may be implemented to any PRRS virus, such as to a PRRS virus selected from the group consisting of wild type PRRS virus, attenuated PRRS virus, modified-life PRRS virus, PRRS virus mutant and combinations thereof. In one example, the mutation as described herein may be implemented to an attenuated and/or live PRRS virus, for example to a PRRS virus selected from the group of the PRRS virus strains that have been deposited on 27 Oct. 2004 with the European Collection of Cell Cultures (ECACC), Porton Down, Salisbury, Wiltshire, SP4 OJG, Great Britain, under the Accession Numbers ECACC 04102703, ECACC 04102702, and ECACC 04102704. Hence, the mutation as described herein can be combined with one or more other mutations, preferably one or more other attenuating mutations, in a PRRS virus.

[0040] In the context of the invention, the term "PPRRS virus" is in particular equivalent with "PRRS virus strain".

[0041] The term "mutation" in the context of the invention is understood as a change in a genomic sequence, in particular in the RNA sequence of a PRRS virus. Since viruses that use RNA as their genetic material have rapid mutation rates, the term "mutation", as mentioned herein, is particularly directed to a genetically engineered change in a genomic sequence, such as by site directed mutagenesis, which in particular results in a virus growing to titers significantly lower than wild type PRRS virus in interferon competent cells and/or in the infected host, when propagated under the same conditions. Moreover, in another preferred embodiment the mutation described herein can also be caused by natural mutation and subsequent isolation of the PRRS virus according to the invention, wherein said isolated virus includes the mutation described herein.

[0042] Preferably, the mutation, as described herein, comprises or consists of one or more point mutations and/or one or more genomic deletions and/or one or more insertions.

[0043] The term "nsp1 protein", as used herein, is directed to the PRRSV nonstructural protein 1.

[0044] The nsp1 protein is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:1 and SEQ ID NO:2 if the PRRS virus according to the invention is a genotype I PRRS virus, or the nsp1 protein is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:3 if the PRRS virus according to the invention is a genotype II PRRS virus.

[0045] In one aspect, the PRRS virus according to the invention preferably also may comprise a mutation in the gene encoding the nsp1 protein selected from the group consisting of SEQ ID NOs 1-3.

[0046] It is hence understood that the PRRS virus according to the invention is a genotype I PRRS virus or a genotype II PRRS virus. It is further understood that the terms "genotype I" and "genotype II" are equivalent to the terms "genotype 1" and "genotype 2" or to the terms "type 1" and "type 2", as frequently used in the literature in the context of PRRSV.

[0047] Sequence identity in the context of the invention is understood as being based on pairwise determined similarity between nucleotide or protein sequences. The determination of percent identity between two sequences is preferably accomplished using a mathematical algorithm, in particular the well-known Smith-Waterman algorithm (Smith and Waterman, M. S. (1981) J Mol Biol, 147(1):195-197). For purposes of the present invention, percent sequence identity of an amino acid sequence is determined using the Smith-Waterman homology search algorithm using an affine 6 gap search with a gap open penalty of 12 and a gap extension penalty of 2, BLOSUM matrix 62. The Smith-Waterman homology search algorithm is taught in Smith and Waterman (1981) Adv. Appl. Math 2:482-489, herein incorporated by reference. A variant may, for example, differ from the reference nsp1, nsp1α, nsp1β or NTD molecule by as few as 1 to 15 amino acid residues, as few as 1 to 10 amino acid residues, such as 6-10, as few as 5, as few as 4, 3, 2, or even 1 amino acid residue. Alternatively, percent identity of a nucleotide sequence is determined using the Smith-Waterman homology search algorithm using a gap open penalty of 25 and a gap extension penalty of 5. Such a determination of sequence identity can be performed using, for example, the DeCypher Hardware Accelerator from TimeLogic Version G, or the sequence identity is determined with the software CLC MAIN WORKBENCH 4.1.1 (CLC BIO).

[0048] The term "having 100% sequence identity", as used herein, is understood to be equivalent to the term "being identical".

[0049] In another preferred embodiment, the mutation in the PRRS virus according to the invention comprises or consists of one or more point mutations and/or one or more genomic deletions and/or one or more insertions.

[0050] In a further preferred embodiment, the PRRS virus according to the invention comprises a mutation in the gene sequences encoding the nsp1α subunit of the nsp1 protein and/or the nsp1β subunit of the nsp1 protein of said virus.

[0051] The term "nsp1α", as mentioned herein, is thus directed to the PRRSV nsp1α subunit of the nsp1 protein, and the term "nsp1β", as used herein, is hence directed to the PRRSV nsp1β subunit of the nsp1 protein.

[0052] The nsp1α is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:4 and SEQ ID NO:5 if the PRRS virus according to the invention is a genotype I PRRS virus, or the nsp1α is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:6 if the PRRS virus according to the invention is a genotype II PRRS virus.

[0053] The nsp1β is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:7 and SEQ ID NO:8 if the PRRS virus according to the invention is a genotype I PRRS virus, or the nsp1β is preferably a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:9 if the PRRS virus according to the invention is a genotype II PRRS virus.

[0054] In a particular preferred embodiment, the PRRS virus according to the invention comprises a mutation in the gene sequence encoding the N-terminal domain (NTD) of the nsp1β of said virus. The term "NTD", as mentioned herein, is thus directed to the N-terminal domain of the PRRSV nsp1β subunit.

[0055] The NTD is preferably a polypeptide or has a polypeptide sequence, respectively, having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide or a polypeptide sequence, respectively, selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11 if the PRRS virus according to the invention is a genotype I PRRS virus, or the NTD is preferably a polypeptide or has polypeptide sequence, respectively, having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide or the polypeptide sequence, respectively, set forth in SEQ ID NO:12 if the PRRS virus according to the invention is a genotype II PRRS virus.

[0056] In a preferred embodiment, the NTD comprising the mutation has the sequence selected from the group consisting of the sequences

TABLE-US-00001 SXXYXXXXXVXFXDXXXXGXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXGXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,

as set forth in SEQ ID NOs:36-41, if the PRRS virus according to the invention is a genotype I PRRS virus, or the NTD comprising the mutation preferably has the sequence selected from the group consisting of the sequences

TABLE-US-00002 AXVYDIGXXAVMXVAXXXXSWAGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSWGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXGGXXXXFXXXXXXLXLXAXXXXXS,

as set forth in SEQ ID NOs: 42-47, if the PRRS virus according to the invention is a genotype II PRRS virus.

[0057] Thus, the PRRS virus according to the invention preferably comprises a gene sequence encoding a NTD selected from the sequences

TABLE-US-00003 SXXYXXXXXVXFXDXXXXGXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXGXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS, SXXYXXXXXVXFXDXXMWXXXSXXSXXLEXLPXXLXXXXXXLXRS,

as set forth in SEQ ID NOs:36-41, if the PRRS virus according to the invention is a genotype I PRRS virus, or the PRRS virus according to the invention preferably comprises a gene sequence encoding a NTD selected from the sequences

TABLE-US-00004 AXVYDIGXXAVMXVAXXXXSWAGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSWGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXSGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXXGGXXXXFXXXXXXLXLXAXXXXXS, AXVYDIGXXAVMXVAXXGGXXXXFXXXXXXLXLXAXXXXXS.

as set forth in SEQ ID NOs: 42-47, if the PRRS virus according to the invention is a genotype II PRRS virus. In one exemplary embodiment, the NTD comprising the mutation may, for instance, have the sequence selected from the group consisting of the sequences

TABLE-US-00005 SSVYRWKKFVVFTDSSXNGRMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNGMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSMMWTPESDDSAXLEXLPPELERQVEILIRS,

as set forth in SEQ ID NOs:48-53, if the PRRS virus according to the invention is a genotype I PRRS virus, or the NTD comprising the mutation may, for instance, have the sequence selected from the group consisting of the sequences

TABLE-US-00006 ATVYDIGXXAVMYVAXXKVSWAGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSWGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXGGXEVKFEXVPXELKLXANRLXTS,

as set forth in SEQ ID NOs: 54-59, if the PRRS virus according to the invention is a genotype II PRRS virus.

[0058] Thus, in one exemplary embodiment, the PRRS virus according to the invention may, for instance, comprise a gene sequence encoding a NTD selected from the sequences

TABLE-US-00007 SSVYRWKKFVVFTDSSXNGRMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNGMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXNMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSXMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSSMMWTPESDDSAXLEXLPPELERQVEILIRS, SSVYRWKKFVVFTDSMMWTPESDDSAXLEXLPPELERQVEILIRS,

as set forth in SEQ ID NOs:48-53, if the PRRS virus according to the invention is a genotype I PRRS virus, or the PRRS virus according to the invention may, for instance, comprise a gene sequence encoding a NTD selected from the sequences

TABLE-US-00008 ATVYDIGXXAVMYVAXXKVSWAGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSWGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVSGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKVGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXKGGXEVKFEXVPXELKLXANRLXTS, ATVYDIGXXAVMYVAXXGGXEVKFEXVPXELKLXANRLXTS,

as set forth in SEQ ID NOs: 54-59, if the PRRS virus according to the invention is a genotype II PRRS virus.

[0059] It is thus understood that a genotype I PRRS virus, as mentioned herein, is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7 and SEQ ID NO:8, or is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:10 and SEQ ID NO:11.

[0060] Preferably, the genotype I PRRS virus, as mentioned herein, comprises or consists of a RNA polynucleotide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a RNA polynucleotide complementary to a polynucleotide selected from the group consisting of SEQ ID NO: 13 and SEQ ID NO: 14.

[0061] It is further understood that a genotype II PRRS virus, as mentioned herein, is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a polypeptide selected from the group consisting of SEQ ID NO:3, SEQ ID NO:6 and SEQ ID NO:9, or is in particular a virus whose genome comprises a gene sequence coding for a polypeptide having at least 60%, particularly at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with the polypeptide set forth in SEQ ID NO:12.

[0062] Preferably, the genotype II PRRS virus, as mentioned herein, comprises or consists of a RNA polynucleotide having at least 70%, preferably at least 80%, more preferably at least 90%, still more preferably at least 95% or in particular 100% sequence identity with a RNA polynucleotide complementary to the polynucleotide set forth in SEQ ID NO: 15.

[0063] In the following, the single letter code for amino acids is used for amino acid sequences, where additionally X signifies any genetically encoded amino acid residue.

[0064] Within the context of the invention it is in particular understood that the N-terminal domain (NTD) of nsp1β starts with the N-terminus of nsp1β, preferably starts with the amino acid sequence SXXY if the PRRS virus is a genotype I PRRS virus or with the amino acid sequence AXVY if the PRRS virus is a genotype II PRRS virus, and the NTD ends with the serine residue (S) within the amino sequence SFP of the nsp1β. Regarding the NTD domain of a genotype II PRRS virus it is in particular referred to the publication Xue et al. 2010, hereby incorporated by reference. Said amino acid sequences SXXY or AXVY and SFP are especially conserved in the nsp1β of wild type PRRS viruses and are thus preferably used amino acid sequence motifs for defining the NTD domain according to the invention.

[0065] In a particular preferred embodiment, the PRRS virus according to the invention thus comprises a mutation in the gene sequence coding for the NTD, wherein the NTD preferably starts with motif SXXY for PRRS virus type 1 strains or with motif AXVY for PRRS virus type 2 strains and ends with the serine residue (S) within the conserved SFP motif of the nsp1β subunit.

[0066] According to the invention, it is in particular preferred if said mutation is located within the part of the gene sequence coding for the NTD, where the amino acid stretches folding into β sheet secondary structures are encoded.

[0067] In one preferred aspect, the mutation, as described herein, thus comprises a deletion or replacement of a nucleotide triplet coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.

[0068] In particular, it is preferred if the mutation described herein comprises of a deletion or replacement of 2 to 7 or more, preferably consecutive, nucleotide triplets each coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.

[0069] In another preferred aspect, the mutation, as mentioned herein, comprises the deletion or replacement of a nucleotide triplet coding for an amino acid residue with a charged, preferably positively charged, side chain, in particular coding for an arginine residue.

[0070] Exemplarily, the mutation mentioned herein preferably consists of a deletion of 2, 3, 4, 5, 6 or 7 consecutive nucleotide triplets coding for the respective number (2, 3, 4, 5, 6 or 7) of consecutive amino acid residues located within the first 24 N-terminal amino acid residues of the NTD sequence, wherein this mutation comprises the deletion of a nucleotide triplet coding for an amino acid residue with a charged, preferably positively charged, side chain, in particular coding for an arginine residue.

[0071] The mutation, as described herein, preferably comprises or consists of a deletion or replacement of the nucleotide triplet coding for the first arginine residue (R) located at least 21 amino acid residues in C-terminal direction from the N-terminal amino acid residue of the nsp1β NTD and/or the mutation, as mentioned herein, comprises a deletion or replacement of the nucleotide triplet coding for the arginine residue (R) of the nsp1 amino acid sequence RXMW if the PRRS virus is a genotype I PRRS virus or with the amino acid sequence RGG of said virus if the PRRS virus is a genotype II PRRS virus and/or the mutation, as mentioned herein, comprises or consists of a deletion or replacement of the nucleotide triplet coding for the arginine residue (R) of the amino acid sequence RMM or RGG of the nsp1 protein.

[0072] According to the invention, the phrase "replacement of a nucleotide triplet" is understood as being a replacement of a nucleotide triplet of the wild type sequence by another nucleotide triplet coding for a different amino acid residue than the wild type sequence.

[0073] The phrase "deletion of nucleotide triplet" in the context of the invention is directed to a deletion of a nucleotide triplet resulting in the deletion of an amino acid residue in comparison with the wild type sequence.

[0074] In particular, said mutation further comprises a deletion or replacement of one nucleotide triplet coding for the amino acid residue flanking said arginine residue (R) in N-terminal direction, wherein said mutation preferably comprises or consists of a deletion or replacement of two consecutive nucleotide triplets coding for the amino acid residues PR.

[0075] More particular, said mutation further comprises a deletion or replacement of two, three, four, five, six, seven or more consecutive nucleotide triplets coding for two, three, four, five, six, seven or more amino acid residues flanking said arginine residue (R) in N-terminal direction.

[0076] Preferably, said mutation comprises a deletion or replacement of three consecutive nucleotide triplets coding for the amino acid sequence RXR or APR of the nsp 1β, or wherein said mutation comprises a deletion or replacement of four consecutive nucleotide triplets coding for the amino acid residues GRXR or WAPR of the nsp 1β.

[0077] As result, the PRRS virus according to the invention comprises in one embodiment a gene coding for a nsp1 protein selected from the group consisting of SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19 and SEQ ID NO:20, or comprises in another embodiment a gene sequence coding for a nsp1β selected from the group consisting of SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24 and SEQ ID NO:25, or comprises in a further embodiment a gene sequence coding for a NTD selected from the group consisting of SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29 and SEQ ID NO:30.

[0078] In yet another preferred embodiment, the mutation, as described herein comprises a deletion of the gene sequence encoding the (whole) nsp1 protein, encoding the (whole) nsp 1α subunit, encoding the (whole) nsp1β subunit, or encoding a part, preferably at least seven consecutive amino acid residues, of or the (whole) NTD domain of the nsp1β subunit.

[0079] Yet another aspect of the invention is directed to a polynucleotide comprising or consisting of the genome of the PPRS virus according to the invention.

[0080] Further, the invention is also directed to a virus particle, wherein said virus particle comprises a polynucleotide which comprises or consists of the genome of the PPRS virus according to the invention or which comprises or consists of a DNA copy of the PPRS virus according to the invention.

[0081] Still further, the present invention provides a DNA-Vector comprising a copy of, or a cDNA sequence complementary to, respectively, a polynucleotide which comprises or consists of the genome of the PPRS virus according to the invention.

[0082] In one exemplary and non-limiting example the DNA vector, as mentioned herein, comprises a polynucleotide sequence selected from the group consisting of SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34 and SEQ ID NO:35.

[0083] Also, the present invention provides a cell comprising a polynucleotide, which comprises or consists of the genome of the PPRS virus according to the invention or which comprises or consists of a DNA copy of the PPRS virus according to the invention.

[0084] In one exemplary and non-limiting example the PRRS virus according to the invention, or the genome of the PRRS virus according to the invention, respectively, comprises a RNA sequence or consists of a RNA polynucleotide, wherein said RNA sequence or RNA polynucleotide is a RNA copy of a polynucleotide selected from the group consisting of SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34 and SEQ ID NO: 35.

[0085] Another aspect of the invention concerns the PRRS virus according to the invention for use as vaccine or medicament, in particular for use in the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome preferably in swine. The present invention further provides a composition containing the PRRS virus according to the invention for use as a vaccine or as a medicament, in particular for use in the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome preferably in swine.

[0086] In a preferred aspect, said vaccine or medicament is administered in two doses or preferably in one dose to an animal in need thereof. By the term "prophylaxis or therapy", as mentioned herein, it is meant that the prophylaxis is to administer a drug, in particular a vaccine, before exposure to PRRSV and the treatment is to administer a drug, in particular a medicament, after infection with PRRSV or onset of PRRS.

[0087] In particular, the vaccine, as mentioned herein, is a live vaccine and/or a modified live vaccine and/or an attenuated live or attenuated modified live vaccine.

[0088] The strains of the PRRS virus according to the invention can be grown and harvested by methods known in the art, e.g. by propagating in suitable cells like the simian cell line MA-104, Vero cells, or porcine alveolar macrophages. Preferably, vaccines according to the present invention are modified live vaccines comprising one or more of these strains alive in a suitable carrier, but inactivated virus may also be used to prepare killed vaccine (KV). Modified live vaccines (MLV) are typically formulated to allow administration of 10¹ to 10⁷ viral particles per dose, preferably 10³ to 10⁶ particles per dose, more preferably 10⁴ to 10⁶ particles per dose (4.0-6.0 log₁₀ TCID₅₀). KV may be formulated based on a pre-inactivation titre of 10³ to 10¹0 viral particles per dose. The vaccine may comprise a pharmaceutically acceptable carrier, for example a physiological salt-solution. The vaccine may or may not comprise an adjuvant. A suitable adjuvant may optionally also be used. For example, a suitable adjuvant that may be used a-tocopherol acetate which may be obtained under the trade name Diluvac Forte®. Alternatively, alum based adjuvants may be used.

[0089] A further aspect of the invention is thus directed to the use of the PRRS virus according to the invention for the preparation of a vaccine or medicament for the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome, preferably in swine.

[0090] A vaccine according to the present invention may be presented in form of a freeze-dried preparation of the live virus, to be reconstituted with a solvent, to result in a solution for injection. The solvent may, for example, be water, physiological saline, a buffer, or an adjuvanting solvent. The solvent may additionally use adjuvants, for example a-tocopherol acetate. The reconstituted vaccine may then be injected into a pig, for example as an intramuscular or intradermal injection into the neck. For intramuscular injection, a volume of 2 ml may be applied, for an intradermal injection it is typically 0.2 ml. In a further aspect, the present invention therefore is a product, comprising in separate containers a freeze-dried composition of the virus, and a solvent for reconstitution, and optionally further containing a leaflet or label comprising instructions of use.

[0091] Yet another aspect of the invention thus concerns a method for the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome comprising administering the PRRS virus according to the invention to an animal, preferably to a swine, more preferably to a pig, in particular preferably to a piglet or a sow.

[0092] A vaccine according to the present invention may not only comprise the PRRS virus according to the invention as antigen, but may include further components active against PRRS or other porcine viral or bacterial diseases, like porcine circovirus, classical swine fever virus or mycoplasma hyorhinis. Therefore, the invention further relates to a vaccine as described, characterized in that it contains at least one further antigen active against a porcine disease which is not PRRS.

[0093] Still another aspect of the invention thus concerns a medicament or vaccine comprising the PRRS virus according to the invention.

[0094] Still a further aspect of the invention is directed to a method of preparing a vaccine or medicament, in particular for the prophylaxis or treatment of Porcine Reproductive and Respiratory Syndrome preferably in swine, said method comprising the cultivation of the PRRS virus according to the invention in cell culture.

[0095] Moreover, the invention comprises a method of producing the PRRS virus according to the invention, said method comprising the mutagenesis of the gene coding for the nsp1 protein in the genome of a PRRS virus, and wherein the mutation, as described herein, is in particular introduced by genetic engineering in the genome of said virus, preferably by site directed mutagenesis.

[0096] Preferably, for any of the aforementioned methods, the above-mentioned DNA-vector is used and/or said methods comprise the detection of interferon type I, in particular interferon type I secretion, and/or of mRNA coding for interferon type I. Said detection in particular comprises the detection of interferon type I and/or of mRNA coding for interferon type I by a bioassay, wherein said bioassay is preferably selected from the group consisting of ELISA, PCR, GLISA, IFA, biosensoric measurement, Surface Plasmon Resonance (SPR) measurement, selective media, lateral flow, biochip measurement, immunomagnetic separation, electrochemiluminescence, chromogenic media, immunodiffusion, DNA hybridization, staining, colorimetric detection, luminescence, and combinations thereof.

[0097] In yet a further aspect, the invention provides an immunogenic composition containing the PRRS virus according to the invention.

[0098] As used herein, the term "immunogenic composition" in particular refers to a composition that will elicit an immune response in an animal that has been exposed to the composition. An immune response may include induction of antibodies and/or induction of a T-cell response.

[0099] Usually, an "immune response" includes but is not limited to one or more of the following effects: the production or activation of antibodies, B cells, helper T cells, suppressor T cells, and/or cytotoxic T cells, directed specifically to an antigen or antigens included in the composition or vaccine of interest. Preferably, the host will display either a therapeutic or a protective immunological (memory) response such that resistance to new infection will be enhanced and/or the clinical severity of the disease reduced. Such protection will be demonstrated by either a reduction in number or severity of, or lack of one or more of the symptoms associated with the infection of the pathogen, in the delay of onset of viremia, in a reduced viral persistence, in a reduction of the overall viral load and/or in a reduction of viral excretion. Thus, an "immune response" in particular means but is not limited to the development in a subset of a cellular and/or antibody-mediated immune response to the composition or vaccine of interest.

[0100] In one aspect, the immunogenic composition of the invention is preferably a vaccine or medicament.

[0101] As used herein, the term "viremia" is particularly understood as a condition in which PRRS virus particles circulate in the bloodstream of an animal.

[0102] The term "animal", as mentioned herein, is in particular directed to swine, more particular to a pig, preferably a domestic pig.

[0103] In a preferred aspect, the immunogenic composition of the invention comprises an amount of 10¹ to 10⁷ viral particles of the PRRS virus according to the invention per dose, preferably 10³ to 10⁶ particles per dose, more preferably 10⁴ to 10⁶ particles per dose.

[0104] In another preferred aspect, the immunogenic composition of the invention comprises an amount of the PRRS virus according to the invention which is equivalent to a virus titre of at least about 10³ TCID₅₀/mL per dose, preferably between 10³ to 10⁵ TCID₅₀/mL per dose

[0105] In yet another preferred aspect, the immunogenic composition of the invention further contains one or more pharmaceutically acceptable carriers or excipients. Said one or more pharmaceutically acceptable carriers or excipients are preferably selected from the group consisting of solvents, dispersion media, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, and adsorption delaying agents.

[0106] The present invention is further directed to the immunogenic composition of the invention for use in a method for [0107] inducing an immune response against PRRSV, or [0108] the prevention or reduction of PRRS, or [0109] the prevention or reduction of PRRSV viremia and/or [0110] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or [0111] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal.

[0112] As used herein, the term "inducing an immune response" to an antigen or composition is the development of a humoral and/or cellular immune response in an animal to an antigen present in the composition of interest.

[0113] The term "prevention" or "reduction" or "preventing" or "reducing", respectively, as used herein, means, but is not limited to a process which includes the administration of a PRRSV antigen, namely of the PRRSV according to the invention which is included in the composition of the invention, to an animal, wherein said PRRSV antigen, when administered to said animal elicits or is able to elicit an immune response in said animal against PRRSV. Altogether, such treatment results in reduction of the clinical symptoms of PRRS or of symptoms associated with PRRSV infection, respectively. More specifically, the term "prevention" or "preventing, as used herein, means generally a process of prophylaxis in which an animal is exposed to the immunogenic composition of the present invention prior to the induction or onset of the disease process (PRRS).

[0114] Herein, "reduction of PRRS" or "reduction of clinical symptoms associated with PRRSV infection" means, but is not limited to, reducing the number of infected subjects in a group, reducing or eliminating the number of subjects exhibiting clinical symptoms of infection, or reducing the severity of any clinical symptoms that are present in the subjects, in comparison to wild-type infection. For example, it should refer to any reduction of pathogen load, pathogen shedding, reduction in pathogen transmission, or reduction of any clinical sign symptomatic of PRRSV infection, in particular of elevated body temperature. Preferably these clinical symptoms are reduced in subjects receiving the composition of the present invention by at least 10% in comparison to subjects not receiving the composition and may become infected. More preferably, clinical symptoms are reduced in subjects receiving the composition of the present invention by at least 20%, preferably by at least 30%, more preferably by at least 40%, and even more preferably by at least 50%.

[0115] Also, the elevated body temperature usually associated with the administration of an attenuated PRRSV vaccine to an animal is reduced in subjects receiving the composition of the present invention by at least 10% in comparison to subjects receiving a conventional attenuated PRRSV vaccine. More preferably, the elevated body temperature usually associated with the administration of an attenuated PRRSV vaccine is reduced in subjects receiving the composition of the present invention by at least 20%, preferably by at least 30%, more preferably by at least 40%, and even more preferably by at least 50%.

[0116] Hence, a further advantage of the PRRS virus according to the invention is in particular the effect that its administration to an animal, e.g. for the prophylaxis or treatment of PRRS, results in a reduced increase of body temperature in the animal in comparison with a conventional attenuated PPRSV vaccine.

[0117] The term "conventional attenuated PRRSV vaccine", as mentioned herein, is in particular directed to any attenuated PRRSV useful as a vaccine, wherein said PRRSV does not have the characteristic features of the PRRS virus of the present invention, in particular is not able to induce the interferon type I production and secretion by a cell infected by said PRRSV.

[0118] The term "subject", as mentioned herein, in particular relates to an animal.

[0119] The term "body temperature", as used herein, in particular refers to the approximate average normal, internal temperature of an animal, for example about 38.5-39° C. in pigs, whereas the body temperature associated with a PRRSV infection may be elevated up to 41° C. in pigs.

[0120] The term "reduction of PRRSV viremia" means, but is not limited to, the reduction of PRRS virus entering the bloodstream of an animal, wherein the viremia level, i.e. the number of PRRSV RNA copies per mL of blood serum or the number of plaque forming colonies per deciliter of blood serum, is reduced in the blood serum of subjects receiving the composition of the present invention by at least 50% in comparison to subjects not receiving the composition and may become infected. More preferably the viremia level is reduced in subjects receiving the composition of the present invention by at least 90%, preferably by at least 99.9%, more preferably by at least 99.99%, and even more preferably by at least 99.999%.

[0121] The present invention further relates to the use of the PRRS virus according to the invention or of the immunogenic composition of the invention for the preparation of a vaccine or medicament for [0122] inducing an immune response against PRRSV, or [0123] treating or preventing PRRS, or [0124] preventing or reducing PRRSV viremia and/or [0125] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or [0126] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal.

[0127] In particular, it is preferred if the vaccine or medicament, as mentioned herein, is to be administered in two doses or preferably in a single dose to an animal.

[0128] The present invention also provides a method of preparing an immunogenic composition, preferably a vaccine or medicament, more preferably a vaccine or medicament for [0129] inducing an immune response against PRRSV, or [0130] treating or preventing PRRS, or [0131] preventing or reducing PRRSV viremia and/or [0132] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or [0133] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal, wherein said method comprises the step of mixing the PRRS virus according to the invention with one or more pharmaceutically acceptable carriers or excipients, and wherein said one or more pharmaceutically acceptable carriers or excipients are preferably selected from the group consisting of solvents, dispersion media, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, and adsorption delaying agents.

[0134] In another aspect, the present invention further provides a method for [0135] inducing an immune response against PRRSV, or [0136] treating or preventing PRRS, or [0137] preventing or reducing PRRSV viremia and/or [0138] preventing or reducing clinical symptoms, in particular the elevated body temperature, associated with PRRSV infection, or [0139] the prevention or reduction of the elevated body temperature associated with the administration of an attenuated PRRSV vaccine to an animal, wherein said method comprises the step of administering the immunogenic composition of the invention to an animal in need thereof, and wherein preferably the immunogenic composition and/or the vaccine is administered in two doses or, more preferably, in a single dose.

EXAMPLES

[0140] In the examples five viable, genetically designed PRRSV mutant strains are described which are based on infectious EU PRRSV cDNA clone LoN94-13. These strains, delta nsp1 IX-10, delta nsp1 XVII-1, delta nsp1 XVIII-12, delta nsp1 XIX-2 and delta nsp1 XX-9 (henceforth referred to as vaccine candidates), harbor genomic deletions of two, three, four, five, or six codons in their predicted nsp1 genes, respectively, resulting in deletions of two (motif P21R22), three (motif R20P21R22), four (motif G19R20P21R22), five (motif N18G19R20P21R22), or six (motif (P17N18G19R20P21R22) amino acids in their predicted nsp1β proteins, respectively (FIG. 1).

[0141] Based on sequence alignments of parental strain LoN94-13 with PRRSV US and EU reference strains VR-2332 and Lelystad virus as well as with strain GD-XH, the deletions are located in the predicted nsp1β portion of nsp 1 (FIG. 2). In more detail, the deletion site for all vaccine candidates is located in the N-terminal domain (NTD) of nsp1β and overlaps with amino acids P23R24 of GD-XH nsp1β; (FIG. 2).

[0142] After transfection of synthetic transcripts of the vaccine candidates into BHK21 cells and transfer of cell culture supernatant from transfected BHK21 cells onto PRRSV-susceptible MA104 cells, plaque formation typical for PRRSV infection occurred. PRRSV-specificity and viability for each of the vaccine strains then was demonstrated by subsequent cell culture passages on MA104 cells and PRRSV-specific immunofluorescence using monoclonal antibody SDOW17 (Rural Technologies).

[0143] After endpoint dilution and generation of virus stocks each derived from material of a single virus plaque, virus titers of the obtained virus stocks were determined for each vaccine candidate by serial virus titrations on 96-well plates containing MA104 cells followed by PRRSV-specific immunofluorescence analyses six to seven days post infection. Unlike experience with titrations of virus stocks from parental PRRSV LoN94-13, the first serial dilutions of vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 did not demonstrate a cytopathic effect and virus plaque formation, while at higher dilutions of the virus stocks a cytopathic effect was detectable. Moreover, when respective titrations were investigated by immunofluorescence, cell culture wells of the first serial dilutions were negative for PRRSV infection for both vaccine candidates, while wells infected with higher dilutions of the virus stocks showed PRRSV-specific immunofluorescence, respectively (FIG. 3).

[0144] To determine whether the prepared vaccine candidate virus stocks contained type I IFN, a commercial ELISA specific for human IFNβ (Invitrogen) was used. MA104 cells are epithelial Green Monkey kidney cells. According to the ELISA manufacturer, this Invitrogen ELISA is also suited for the detection of primate IFNβ other than human. For each vaccine candidate's virus stock, 100 μl served as assay input, while a virus stock from parental strain LoN94-13, cell culture medium, and medium from noninfected cells served as controls. For quantification of the obtained results, a calibration curve was included using a positive control of the ELISA manufacturer. All samples were measured in duplicates. Unlike the negative controls, virus stocks of the vaccine candidates contained considerable levels of type I IFN, while the virus stock of the parental virus showed IFN levels as low as the negative controls (FIG. 4).

[0145] To confirm the results obtained and to assess kinetics of type I IFN production in cells infected with the vaccine candidates, a time course experiment was performed using MA104 cells infected at a multiplicity of infection (MOI) of 0.001, respectively. Parental strain LoN94-13 served as negative control. While there were only very little and unaltered levels of type I IFN near background detectable for infection with parental strain LoN94-13, vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 induced considerable and increasing amounts of up to about 18 I.U. IFNβ per 25 μl sample volume from two days post infection on (FIG. 5).

[0146] It was experimentally assessed whether vaccine candidates containing genomic deletions in nsp1 demonstrate an increased sensitivity to type I IFN (FIG. 6). 5×10⁵ MA104 cells were seeded into a well of a six-well plate and were either not infected (n.inf.) or infected with 800 infectious virus particles of one of the virus strains given on top, respectively. Cells then were either inoculated with 120 I.U. human IFNβ (+IFNβ, bottom row), respectively, or not (-IFNβ, top row). Three days post infection, immunofluorescence analysis specific for the PRRSV capsid protein was performed using monoclonal antibody SDOW17 (Rural Technologies). The total numbers of foci of PRRSV-infected cells per well are given below, respectively.

[0147] This experiment demonstrated that inoculation with type I IFN reduced the number of PRRSV infection events in cells after inoculation with a defined number of infectious virus particles, reflecting reduced viral infectivity of PRRSV when IFN was added. This reduction was 80-fold for wild type virus LoN94-13 (FIG. 6). In addition, for infection with wild type virus, foci of infected cells were smaller than in the well not inoculated with IFN (FIG. 6). However, for vaccine strains delta nsp1 XVII-1 and delta nsp1 XVIII-12, viral infectivity was reduced to zero when INF was added (FIG. 6). Thus, these vaccine candidates not only induce production of type I IFN in infected cells (FIG. 4 and FIG. 5), but also demonstrate increased sensitivity to type I INF when compared to wild type PRRSV (FIG. 6). This is reflected by their dramatically reduced viral infectivity when IFNβ is present.

[0148] Interestingly, the cells infected for the time course experiment summarized in FIG. 5 not only produced considerable amounts of IFNβ, but at the end of the experiment at six days post infection, cells infected with vaccine candidates delta nsp1 XVII-1 and delta nsp1 XVIII-12 showed signs of recovery from usually lytical PRRSV infection. While cells infected with parental strain LoN94-13 were fully lysed, cells infected with the vaccine candidates grew in a partially (delta nsp1 XVIII-12) or completely intact monolayer (delta nsp1 XVII-1). For the latter, only weak signs of a PRRSV-induced cytopathic effect were still detectable. Thus, the interferon production of infected cells together with the observed sensitivity of vaccine candidates to type I IFN correlated with partial or almost complete recovery of infected cells over time. It is reasonable to expect that type I IFN induction by the vaccine candidates together with their increased sensitivity to type I IFN will contribute to a significantly attenuated viral phenotype in the natural host. In particular, expected features of the vaccine candidates' attenuation in pigs include stimulation of the innate and specific immunity, both humoral and cellular, and less shedding and/or shortened viremia of the vaccine viruses.

[0149] To assess whether the PRRSV vaccine candidates are attenuated in the host, an animal experiment in piglets was performed.

[0150] Three groups, each of ten animals, were infected at study day 0 either with wild-type parental EU PRRSV strain Lon94-13 (WT group), or with delta nsp1 XVIII-12 (nsp1 group), or were not infected (Ch control group). Infection was applied by intramuscular injection to the neck at dosages of 10^6,56 TCID₅₀ for LoN94-13 or 10^6,6 TCID₅₀ for delta nsp1 XVIII-12, respectively. 21 days post vaccination, all animals were challenged with a virulent EU PRRSV strain being heterologous to LoN94-13 by intramuscular injection and intranasal inoculation at a total dosage of 3×10^6,52 TCID₅₀. Animals were kept until the end of the experiment at day 31, ten days after challenge, and body temperatures were measured for all animals at days 0 (1 and 4 hours post vaccination), 1, 3, 5, 8, 10, 12, 14, 18, 20, 22, 24, 26, 28, and 31.

[0151] Mean body temperatures were determined for each animal for the time after vaccination but before challenge using measured body temperature data from all timepoints from day 0 through day 20. Subsequently, mean body temperatures were determined for each group (FIG. 7, (left-hand) columns). Error bars indicate standard deviations, respectively. Following the same procedure, mean body temperatures and standard deviations were determined for all groups for the time after challenge using measured body temperature data from all timepoints from day 22 through 31 (FIG. 7, (right-hand) columns).

[0152] The term "Significant(ly)" in the context of the following means either (i) p-values of 0.05 or lower as determined by the Dunnett test and obtained from comparing the nsp1 group with either the WT or the Ch control group for either of the two time periods investigated (before and after challenge) or (ii) p-values of 0.05 or lower when comparing the mean temperature change within a group and between the two time periods investigated (before and after challenge).

[0153] When comparing the determined mean body temperatures for the time after vaccination but before challenge (left hand columns) in between the three groups, animals from the WT group demonstrated a rise in body temperature of more than 0.4° C. when compared to animals from the noninfected Ch control group, thus demonstrating virulence of LoN94-13 in the infected host. In contrast, the nsp1 group showed a significant reduction in mean body temperature of more than 0.2° C. when compared to the WT group. Thus, since vaccination dosages were the same for the WT and the nsp1 group, the considerable reduction in increase of body temperature compared to WT demonstrates that the described mutation in the genome of delta nsp1 XVIII-12 has significantly reduced virulence of the WT parental strain LoN94-13 in the infected animal.

[0154] The significant rise in the mean body temperature of the Ch control group from before challenge to after challenge of 0.4° C. demonstrates virulence of the heterologous EU PRRSV challenge strain. The mean temperature of the WT group after challenge was slightly lower than before challenge, but not significantly reduced (FIG. 7). In contrast, the mean body temperature of the nsp1 group after challenge was significantly reduced by almost 0.2° C. when compared to that before challenge. Moreover, the body temperature of the nsp1 group after challenge was significantly reduced by almost 0.4° C. when compared to the Ch control group after challenge. Also, mean body temperature of the nsp1 group after challenge was significantly lower that that of the WT group after challenge by more than 0.2° C. Taken together, this demonstrates that a measurable and significant degree of protection from signs of disease induced by the applied challenge virus was conferred to pigs by vaccination with delta nsp1 XVIII-12. Since parental PRRSV strain LoN94-13 did not confer significant protection, it is evident that the described mutation in the genome of delta nsp1 XVIII-12 is causative for the observed significant protective technical effect.

[0155] Analogous experiments, wherein the vaccination was performed with lower amounts (10⁵ TCID₅₀) of delta nsp1 XVIII-12 showed results similar to the above described results (data not shown). Thus, in practice, a preferred amount of 10³ to 10⁵ TCID₅₀ is sufficient for vaccination.

[0156] Taken together, the invention described here represents the first known viable PRRSV (EU) strains that contain mutations (deletions) in the nsp1 gene (nsp1β) that induce type I IFN (IFNβ) production in susceptible cells (MA104) and that show increased sensitivity to type I IFN (IFNβ). Moreover, the animal data demonstrates that (i) vaccine candidate delta nsp1 XVIII-12 is significantly attenuated in the host when compared to its parental PRRSV strain LoN94-13 and that (ii) vaccine candidate delta nsp1 XVIII-12 confers significant protection from signs of disease induced by challenge with a heterologous PRRSV strain while parental strain LoN94-13 does not. Thus, the described vaccine candidates or the described mutations therein, either alone or combined with other attenuating mutations, may serve as promising life attenuated PRRSV vaccines.

IN THE SEQUENCE LISTING

[0157] SEQ ID NO: 1 corresponds to LoN94-13 complete nsp1 protein,

[0158] SEQ ID NO: 2 corresponds to Lelystad virus complete nsp1 protein,

[0159] SEQ ID NO: 3 corresponds to VR2332 complete nsp1 protein,

[0160] SEQ ID NO: 4 corresponds to LoN94-13 complete nsp1 Alpha,

[0161] SEQ ID NO: 5 corresponds to Lelystad virus complete nsp1 Alpha,

[0162] SEQ ID NO: 6 corresponds to VR2332 complete nsp1 Alpha,

[0163] SEQ ID NO: 7 corresponds to LoN94-13 complete nsp1 Beta,

[0164] SEQ ID NO: 8 corresponds to Lelystad virus complete nsp1 Beta,

[0165] SEQ ID NO: 9 corresponds to VR2332 complete nsp1 Beta,

[0166] SEQ ID NO: 10 corresponds to LoN94-13 nsp1 Beta NTD,

[0167] SEQ ID NO: 11 corresponds to Lelystad virus nsp1 Beta NTD,

[0168] SEQ ID NO: 12 corresponds to VR2332 nsp1 Beta NTD,

[0169] SEQ ID NO: 13 corresponds to LoN94-13 complete viral cDNA insert,

[0170] SEQ ID NO: 14 corresponds to Lelystad virus complete genome,

[0171] SEQ ID NO: 15 corresponds to VR2332 complete genome,

[0172] SEQ ID NO: 16 corresponds to delta nsp1 IX-10 complete nsp1 protein sequence,

[0173] SEQ ID NO: 17 corresponds to delta nsp1 XVII-1 complete nsp1 protein sequence,

[0174] SEQ ID NO: 18 corresponds to delta nsp1 XVIII-12 complete nsp1 protein sequence,

[0175] SEQ ID NO: 19 corresponds to delta nsp1 XIX-2 complete nsp1 protein sequence,

[0176] SEQ ID NO: 20 corresponds to delta nsp1 XX-9 complete nsp1 protein sequence,

[0177] SEQ ID NO: 21 corresponds to delta nsp1 IX-10 complete nsp1Beta protein sequence,

[0178] SEQ ID NO: 22 corresponds to delta nsp1 XVII-1 complete nsp1Beta protein sequence,

[0179] SEQ ID NO: 23 corresponds to delta nsp1 XVIII-12 complete nsp1Beta protein sequence,

[0180] SEQ ID NO: 24 corresponds to delta nsp1 XIX-2 complete nsp1Beta protein sequence,

[0181] SEQ ID NO: 25 corresponds to delta nsp1 XX-9 complete nsp1Beta protein sequence,

[0182] SEQ ID NO: 26 corresponds to delta nsp1 IX-10 nsp1Beta NTD protein sequence,

[0183] SEQ ID NO: 27 corresponds to delta nsp1 XVII-1 nsp1Beta NTD protein sequence,

[0184] SEQ ID NO: 28 corresponds to delta nsp1 XVIII-12 nsp1Beta NTD protein sequence,

[0185] SEQ ID NO: 29 corresponds to delta nsp1 XIX-2 nsp1Beta NTD protein sequence,

[0186] SEQ ID NO: 30 corresponds to delta nsp1 XX-9 nsp1Beta NTD protein sequence,

[0187] SEQ ID NO: 31 corresponds to delta nsp1 IX-10 complete viral cDNA insert sequence,

[0188] SEQ ID NO: 32 corresponds to delta nsp1 XVII-1 complete viral cDNA insert sequence,

[0189] SEQ ID NO: 33 corresponds to delta nsp1 XVIII-12 complete viral cDNA insert sequence,

[0190] SEQ ID NO: 34 corresponds to delta nsp1 XIX-2 complete viral cDNA insert sequence,

[0191] SEQ ID NO: 35 corresponds to delta nsp1 XX-9 complete viral cDNA insert sequence,

[0192] SEQ ID NO: 36 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 2 aa),

[0193] SEQ ID NO: 37 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 3 aa),

[0194] SEQ ID NO: 38 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 4 aa),

[0195] SEQ ID NO: 39 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 5 aa),

[0196] SEQ ID NO: 40 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 6 aa),

[0197] SEQ ID NO: 41 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 7 aa),

[0198] SEQ ID NO: 42 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 2 aa),

[0199] SEQ ID NO: 43 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 3 aa),

[0200] SEQ ID NO: 44 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 4 aa),

[0201] SEQ ID NO: 45 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 5 aa),

[0202] SEQ ID NO: 46 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 6 aa),

[0203] SEQ ID NO: 47 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 7 aa),

[0204] SEQ ID NO: 48 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 2 aa),

[0205] SEQ ID NO: 49 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 3 aa),

[0206] SEQ ID NO: 50 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 4 aa),

[0207] SEQ ID NO: 51 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 5 aa),

[0208] SEQ ID NO: 52 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 6 aa),

[0209] SEQ ID NO: 53 corresponds to a (type 1) nsp1 Beta NTD with mutation (deletion of 7 aa),

[0210] SEQ ID NO: 54 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 2 aa),

[0211] SEQ ID NO: 55 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 3 aa),

[0212] SEQ ID NO: 56 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 4 aa),

[0213] SEQ ID NO: 57 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 5 aa),

[0214] SEQ ID NO: 58 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 6 aa),

[0215] SEQ ID NO: 59 corresponds to a (type 2) nsp1 Beta NTD with mutation (deletion of 7 aa).

REFERENCE LIST

[0216] Allende, R., Laegreid, W. W., Kutish, G. F., Galeota, J. A., Wills, R. W., Osorio, F. A., 2000. Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. J. Virol. 74, 10834-10837. [0217] Ansari, I. H., Kwon, B., Osorio, F. A., Pattnaik, A. K., 2006. Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. J. Virol. 80, 3994-4004. [0218] Beura, L. K., Sarkar, S. N., Kwon, B., Subramaniam, S., Jones, C., Pattnaik, A. K., Osorio, F. A., 2010. Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation. J. Virol. 84, 1574-1584. [0219] Buddaert, W., Van, R. K., Pensaert, M., 1998. In vivo and in vitro interferon (IFN) studies with the porcine reproductive and respiratory syndrome virus (PRRSV). Adv. Exp. Med. Biol. 440, 461-467. [0220] Chen, Z., Lawson, S., Sun, Z., Zhou, X., Guan, X., Christopher-Hennings, J., Nelson, E. A., Fang, Y., 2010. Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology 398, 87-97. [0221] den Boon, J. A., Faaberg, K. S., Meulenberg, J. J., Wassenaar, A. L., Plagemann, P. G., Gorbalenya, A. E., Snijder, E. J., 1995. Processing and evolution of the N-terminal region of the arterivirus replicase ORF1a protein: identification of two papainlike cysteine proteases. J. Virol. 69, 4500-4505. [0222] Haller, O., Weber, F., 2009. The interferon response circuit in antiviral host defense. Verh. K. Acad. Geneeskd. Belg. 71, 73-86. [0223] Kim, O., Sun, Y., Lai, F. W., Song, C., Yoo, D., 2010. Modulation of type I interferon induction by porcine reproductive and respiratory syndrome virus and degradation of CREB-binding protein by non-structural protein 1 in MARC-145 and HeLa cells. Virology 402, 315-326. [0224] Kroese, M. V., Zevenhoven-Dobbe, J. C., Bos-de Ruijter, J. N., Peeters, B. P., Meulenberg, J. J., Cornelissen, L. A., Snijder, E. J., 2008. The nsp1alpha and nsp1 papain-like autoproteinases are essential for porcine reproductive and respiratory syndrome virus RNA synthesis. J. Gen. Virol. 89, 494-499. [0225] Li, H., Zheng, Z., Zhou, P., Zhang, B., Shi, Z., Hu, Q., Wang, H., 2010. The cysteine protease domain of porcine reproductive and respiratory syndrome virus non-structural protein 2 antagonizes interferon regulatory factor 3 activation. J. Gen. Virol. 91, 2947-2958. [0226] Lopez, O. J., Osorio, F. A., 2004. Role of neutralizing antibodies in PRRSV protective immunity. Vet. Immunol. Immunopathol. 102, 155-163. [0227] Meier, W. A., Galeota, J., Osorio, F. A., Husmann, R. J., Schnitzlein, W. M., Zuckermann, F. A., 2003. Gradual development of the interferon-gamma response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination. Virology 309, 18-31. [0228] Miller, L. C., Laegreid, W. W., Bono, J. L., Chitko-McKown, C. G., Fox, J. M., 2004. Interferon type I response in porcine reproductive and respiratory syndrome virus-infected MARC-145 cells. Arch. Virol. 149, 2453-2463. [0229] Ostrowski, M., Galeota, J. A., Jar, A. M., Platt, K. B., Osorio, F. A., Lopez, O. J., 2002. Identification of neutralizing and normeutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J. Virol. 76, 4241-4250. [0230] Shi, X., Wang, L., Li, X., Zhang, G., Guo, J., Zhao, D., Chai, S., Deng, R., 2011a. Endoribonuclease activities of porcine reproductive and respiratory syndrome virus nsp11 was essential for nsp11 to inhibit IFN-beta induction. Mol. Immunol. 48, 1568-1572. [0231] Shi, X., Wang, L., Zhi, Y., Xing, G., Zhao, D., Deng, R., Zhang, G., 2010. Porcine reproductive and respiratory syndrome virus (PRRSV) could be sensed by professional beta interferon-producing system and had mechanisms to inhibit this action in MARC-145 cells. Virus Res. 153, 151-156. [0232] Shi, X., Zhang, G., Wang, L., Li, X., Zhi, Y., Wang, F., Fan, J., Deng, R., 2011b. The Nonstructural Protein 1 Papain-Like Cysteine Protease Was Necessary for Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1 to Inhibit Interferon-beta Induction. DNA Cell Biol. 30, 355-362. [0233] Snijder, E. J., Meulenberg, J. J., 1998. The molecular biology of arteriviruses. J. Gen. Virol. 79 (Pt 5), 961-979. [0234] Song, C., Krell, P., Yoo, D., 2010. Nonstructural protein 1alpha subunit-based inhibition of NF-kappaB activation and suppression of interferon-beta production by porcine reproductive and respiratory syndrome virus. Virology 407, 268-280. [0235] Sun, Y., Xue, F., Guo, Y., Ma, M., Hao, N., Zhang, X. C., Lou, Z., Li, X., Rao, Z., 2009. Crystal structure of porcine reproductive and respiratory syndrome virus leader protease Nsp1alpha. J. Virol. 83, 10931-10940. [0236] Xiao, Z., Batista, L., Dee, S., Halbur, P., Murtaugh, M. P., 2004. The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load. J. Virol. 78, 5923-5933. [0237] Xue, F., Sun, Y., Yan, L., Zhao, C., Chen, J., Bartlam, M., Li, X., Lou, Z., Rao, Z., 2010. The crystal structure of porcine reproductive and respiratory syndrome virus nonstructural protein Nsplbeta reveals a novel metal-dependent nuclease. J. Virol. 84, 6461-6471.

Sequence CWU 1

591385PRTPorcine reproductive and respiratory syndrome virus 1Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu 195 200 205Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg 210 215 220Gln Val Glu Ile Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser225 230 235 240Leu Ala Asp Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe 245 250 255Ser Thr Tyr His Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe 260 265 270Asp Ser Lys Cys Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val 275 280 285Arg Leu Tyr Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys 290 295 300Trp Gly Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly305 310 315 320Ile Arg Ala Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu 325 330 335Ser Cys Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val 340 345 350Thr Pro Gly Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr 355 360 365Glu Pro Thr Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370 375 380Gly3852385PRTPorcine reproductive and respiratory syndrome virus 2Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Phe Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Ser Pro Glu Leu Gln Asp Thr Asp Leu Gly Ala Val Gly 35 40 45Leu Phe Tyr Lys Pro Arg Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Ala65 70 75 80Val Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Ala 100 105 110Pro Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Asn Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Phe Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Leu Asn Gly Arg Ser Arg Met Met Trp Thr Pro Glu 195 200 205Ser Asp Asp Ser Ala Ala Leu Glu Val Leu Pro Pro Glu Leu Glu Arg 210 215 220Gln Val Glu Ile Leu Ile Arg Ser Phe Pro Ala His His Pro Val Asp225 230 235 240Leu Ala Asp Trp Glu Leu Thr Glu Ser Pro Glu Asn Gly Phe Ser Phe 245 250 255Asn Thr Ser His Ser Cys Gly His Leu Val Gln Asn Pro Asp Val Phe 260 265 270Asp Gly Lys Cys Trp Leu Ser Cys Phe Leu Gly Gln Ser Val Glu Val 275 280 285Arg Cys His Glu Glu His Leu Ala Asp Ala Phe Gly Tyr Gln Thr Lys 290 295 300Trp Gly Val His Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Arg Gly305 310 315 320Ile Arg Ala Val Val Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu 325 330 335Ser Cys Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val 340 345 350Thr Pro Gly Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr 355 360 365Glu Pro Thr Thr Ser Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr 370 375 380Gly3853383PRTPorcine reproductive and respiratory syndrome virus 3Met Ser Gly Ile Leu Asp Arg Cys Thr Cys Thr Pro Asn Ala Arg Val1 5 10 15Phe Met Ala Glu Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Leu Asn Leu Gln Val Ser Glu Leu Gly Val Leu Gly 35 40 45Leu Phe Tyr Arg Pro Glu Glu Pro Leu Arg Trp Thr Leu Pro Arg Ala 50 55 60Phe Pro Thr Val Glu Cys Ser Pro Ala Gly Ala Cys Trp Leu Ser Ala65 70 75 80Ile Phe Pro Ile Ala Arg Met Thr Ser Gly Asn Leu Asn Phe Gln Gln 85 90 95Arg Met Ile Arg Val Ala Ala Glu Leu Tyr Arg Ala Gly Gln Leu Thr 100 105 110Pro Ala Val Leu Lys Ala Leu Gln Val Tyr Glu Arg Gly Cys Arg Trp 115 120 125Tyr Pro Ile Val Gly Pro Ala Pro Gly Val Ala Val Tyr Ala Asn Ser 130 135 140Leu His Val Ser Asp Lys Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Leu Pro Leu Pro Gln Arg Pro Lys Pro Glu Asp Phe Cys Pro Phe 165 170 175Glu Cys Ala Met Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val Met 180 185 190Tyr Val Ala Glu Arg Lys Ile Ser Trp Ala Pro Arg Gly Gly Asp Glu 195 200 205Val Lys Phe Glu Ala Val Pro Gly Glu Leu Arg Leu Ile Ala Asn Arg 210 215 220Leu Arg Thr Ser Phe Pro Pro His His Thr Val Asp Met Ser Lys Phe225 230 235 240Ala Phe Thr Ala Pro Gly Cys Gly Val Ser Met Arg Val Glu Arg Gln 245 250 255His Gly Cys Leu Pro Ala Asp Thr Val Pro Glu Gly Asn Cys Trp Trp 260 265 270Ser Leu Phe Asp Ser Leu Pro Leu Glu Val Gln Asn Lys Glu Ile Arg 275 280 285His Ala Asn Gln Phe Gly Tyr Gln Thr Lys His Gly Val Ser Gly Lys 290 295 300Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp305 310 315 320Leu Asn Gly Pro Ile Val Val Gln Tyr Phe Ser Val Lys Glu Ser Trp 325 330 335Ile Arg His Leu Lys Leu Ala Gly Glu Pro Ser Tyr Ser Gly Phe Glu 340 345 350Asp Leu Leu Arg Ile Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Asp 355 360 365Lys Glu Glu Lys Ile Phe Arg Phe Gly Ser His Lys Trp Tyr Gly 370 375 3804180PRTPorcine reproductive and respiratory syndrome virus 4Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His 1805180PRTPorcine reproductive and respiratory syndrome virus 5Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Phe Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Ser Pro Glu Leu Gln Asp Thr Asp Leu Gly Ala Val Gly 35 40 45Leu Phe Tyr Lys Pro Arg Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Ala65 70 75 80Val Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Ala 100 105 110Pro Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Asn Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Phe Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His 1806180PRTPorcine reproductive and respiratory syndrome virus 6Met Ser Gly Ile Leu Asp Arg Cys Thr Cys Thr Pro Asn Ala Arg Val1 5 10 15Phe Met Ala Glu Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Leu Asn Leu Gln Val Ser Glu Leu Gly Val Leu Gly 35 40 45Leu Phe Tyr Arg Pro Glu Glu Pro Leu Arg Trp Thr Leu Pro Arg Ala 50 55 60Phe Pro Thr Val Glu Cys Ser Pro Ala Gly Ala Cys Trp Leu Ser Ala65 70 75 80Ile Phe Pro Ile Ala Arg Met Thr Ser Gly Asn Leu Asn Phe Gln Gln 85 90 95Arg Met Val Arg Val Ala Ala Glu Leu Tyr Arg Ala Gly Gln Leu Thr 100 105 110Pro Ala Val Leu Lys Ala Leu Gln Val Tyr Glu Arg Gly Cys Arg Trp 115 120 125Tyr Pro Ile Val Gly Pro Val Pro Gly Val Ala Val Phe Ala Asn Ser 130 135 140Leu His Val Ser Asp Lys Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Leu Pro Leu Pro Gln Arg Pro Lys Pro Glu Asp Phe Cys Pro Phe 165 170 175Glu Cys Ala Met 1807205PRTPorcine reproductive and respiratory syndrome virus 7Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser 20 25 30Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 35 40 45Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp 50 55 60Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His65 70 75 80Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys 85 90 95Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu 100 105 110Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser 115 120 125Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val 130 135 140Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln145 150 155 160Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe 165 170 175Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr 180 185 190Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195 200 2058205PRTPorcine reproductive and respiratory syndrome virus 8Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Leu Asn Gly Arg Ser Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser 20 25 30Ala Ala Leu Glu Val Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 35 40 45Leu Ile Arg Ser Phe Pro Ala His His Pro Val Asp Leu Ala Asp Trp 50 55 60Glu Leu Thr Glu Ser Pro Glu Asn Gly Phe Ser Phe Asn Thr Ser His65 70 75 80Ser Cys Gly His Leu Val Gln Asn Pro Asp Val Phe Asp Gly Lys Cys 85 90 95Trp Leu Ser Cys Phe Leu Gly Gln Ser Val Glu Val Arg Cys His Glu 100 105 110Glu His Leu Ala Asp Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val His 115 120 125Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Arg Gly Ile Arg Ala Val 130 135 140Val Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln145 150 155 160Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe 165 170 175Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr 180 185 190Ser Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195 200 2059203PRTPorcine reproductive and respiratory syndrome virus 9Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val Met Tyr Val Ala Glu1 5 10 15Arg Lys Ile Ser Trp Ala Pro Arg Gly Gly Asp Glu Val Lys Phe Glu 20 25 30Ala Val Pro Gly Glu Leu Arg Leu Ile Ala Asn Arg Leu Arg Thr Ser 35 40 45Phe Pro Pro His His Thr Val Asp Met Ser Lys Phe Ala Phe Thr Ala 50 55 60Pro Gly Cys Gly Val Ser Met Arg Val Glu Arg Gln His Gly Cys Leu65 70 75 80Pro Ala Asp Thr Val Pro Glu Gly Asn Cys Trp Trp Ser Leu Phe Asp 85 90 95Ser Leu Pro Leu Glu Val Gln Asn Lys Glu Ile Arg His Ala Asn Gln 100 105 110Phe Gly Tyr Gln Thr Lys His Gly Val Ser Gly Lys Tyr Leu Gln Arg 115 120 125Arg Leu Gln Val Asn Gly Leu Arg Ala Val Thr Asp Leu Asn Gly Pro 130 135 140Ile Val Val Gln Tyr Phe Ser Val Lys Glu Ser Trp Ile Arg His Leu145 150 155 160Lys Leu Ala Gly Glu Pro Ser Tyr Ser Gly Phe Glu Asp Leu Leu Arg 165 170 175Ile Arg Val Glu Pro Asn Thr Ser Pro Leu Ala Asp Lys Glu Glu Lys 180 185 190Ile Phe Arg Phe Gly Ser His Lys Trp Tyr Gly 195 2001052PRTPorcine reproductive and respiratory syndrome virus 10Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Gly Arg Pro Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser 20 25 30Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 35 40 45Leu Ile Arg Ser 501152PRTPorcine reproductive and respiratory syndrome virus 11Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Leu Asn Gly Arg Ser Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser 20 25 30Ala Ala

Leu Glu Val Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 35 40 45Leu Ile Arg Ser 501248PRTPorcine reproductive and respiratory syndrome virus 12Ala Thr Val Tyr Asp Ile Gly His Asp Ala Val Met Tyr Val Ala Glu1 5 10 15Arg Lys Ile Ser Trp Ala Pro Arg Gly Gly Asp Glu Val Lys Phe Glu 20 25 30Ala Val Pro Gly Glu Leu Arg Leu Ile Ala Asn Arg Leu Arg Thr Ser 35 40 451315116DNAPorcine reproductive and respiratory syndrome virus 13atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacggtcg gcctcgcatg atgtggacgc 840cggaatccga tgattcagcc gacctagagg cgctaccgcc tgagctagaa cgtcaggtcg 900aaatcctcat tcggagtttt cctgcccatc accctgtcag cctggccgac tgggagctcg 960ctgagtcccc tgagaacggt ttttccttca gcacgtacca ttctggtggt tatcttgtcc 1020aaaaccccga cgtgtttgac agcaagtgct ggctctcctg tttcttggat cagccgatcg 1080aagtgcgcct ctatgaggat tatctggcta acgctttcgg ttaccaaacc aagtggggcg 1140tgtctggtaa gtacctccag cgcaggcttc aagtcaacgg tattcgtgct gtaatcgatc 1200ctgatggccc cattcacgtt gaagcgctgt cttgccccca atcttggatc aggcacctga 1260ctctggacga tgacgtcacc ccaggattcg ttcgcctaac atccctccgc attgtgccga 1320acacagagcc cactactctc cgaatctttc ggtttggagc gcataagtgg tatggcgctg 1380ccggcaaacg ggctcgtgcc aagcgtgccg ctaaaagtga gaagggtccg gctcccactc 1440ccaaggttgc cccgccagcc cccacctgcg gaattgttac ctactctcca ccaacagacg 1500ggtcttgcgg ttggcacgtc cttgccgcca taatgaaccg aatgatgaac ggtgacttca 1560tgtcccctct ggcccagtac aacagaccag aggatgattg ggcttctgat tacgatcttg 1620cccaggcgat tcaatgtctg cggttgcctg ctaccatagt tcggaatcgt gcctgtccca 1680acgccaagta cctcataaaa ctcaacggag tccactggga ggtagaggtg aggtcaggga 1740tggcccctcg ctcccttccc cgcgagtgcg tagtcggcgt ttgttccgaa ggctgtgccg 1800catcgcctta cccagaaaac gggctaccta aacgagcgtt tgaggccttg gcgtctgctt 1860acagactacc ttccgattgt gtcagttctg gtattgctga ctttcttgct aacccccctc 1920aggaattctg gactcttgac aaaatgttga cctccccgtc accggagcgg tctggttttt 1980ccagtctgta caaattgcta ttagaggttg tcccgcagaa atgcggagcc acggaagggg 2040ctttcaccta tgctgttgag agaatgctga aggattgccc gagctccgaa caggccatgg 2100cccttctggc aaaaattaaa gttccatcct caaaggcccc gtctgtgtcc ctggacgagt 2160gtttccctac ggatgtttca gccgatttcg aaccagcatc tcaggagagg tcccaaaatt 2220ccagcgctgc tgttgtcctg cattcaccga atgcaaaaga gttcgaggaa gcagctccag 2280gggaagttca ggagggtggc cacaaggccg tccactctgc actccctgcc gggggtccta 2340acaataagca ggcacagctg gttgccggtg agcaactgaa gctcggcggt tgtggttcgg 2400tagttgggaa tgcacatgaa ggtgttctgg tcccacctgg tccaattaat ttgacaagcg 2460gggatttacc ctcctcaggc tccatgaaag aagatatgct caatagccgg gaggacgaac 2520cactggattt gtcccaacca gcaacagctg tcacaacgac tcttatggga gagctaacac 2580ccgactacct aggttctgat actggtgccc tccccgtcac cgtccgaaaa tttgtcccga 2640cggggcctat actccgtcat gttgagcact gcagcacggg gtcgggcgat agcagttcgc 2700ctttggatct gtctggtgcg caaaccccgg accagccttt aaatctgtcc ctggcggctt 2760ggccagtgag gaccaccgcg tctgatcctg gctgggtcca cggtagacgc gagcctgtct 2820ttgtaaagcc tcgagatgtt ttctctgatg gcgattcagc ccttcagttc ggggagcttt 2880ctgaatccag ctctgtcatc gagtttgatc gggcaaaaga tgctcaggtg gctgacgccc 2940ctgtcggtct gacgacttcg gacgaggccc tctccgcagt cgatcctttc gagttttccg 3000aactcaagcg cccacgtttc tccgcacaag ccttaattga ccgaggcggc ccacttgccg 3060atgtccatgc aaaaataaag aaccgggtat atgaacagtg cctccaagct tgtgagcccg 3120gtagtcgcgc aaccccagct accagggagt ggctcgacaa aatgtgggag agagtggaca 3180tgaaaacctg gcgctgcacc tcacagttcc aagctggtca cattcttgcg tccctcaaat 3240tcctccctga catgattcaa gacacaccgc ctcctgttcc caggaagaac cgagccagtg 3300ataaagctgg cctgaaacaa ctagtggcac agtgggatag gagattgagt tcaacccccc 3360ccccaaaacc ggttgggccg gtacttgacc gggtcgatcc tccgcctacg ggtacccggc 3420aagaagacgt caccccctcc gatgggccac cccatgcgcc ggatggtcga gtgagtacgg 3480gtgggagttg gaaaggcctt atgctttccg gcacccgtct cacggggtcc atcagtcatc 3540gcctcatgac atgggttttt gaagttgtct cccatctccc agcttttatg ctcacacttt 3600tctcgccgcg gggctctatg gctccaggcg attgggtttt tgcaggtgtt gttttacttg 3660ctctcctgct ctgtcgttct tacccaatat tcgggtgcct tcccttattg ggtgtctttt 3720ctggtactgt gcggcgtgtt cgtctgggtg tttttggctc ttggatggct tttgctgtat 3780ttttattctc gactccatcc aacccagtcg gttcttcttg taaccacgat tcgccggagt 3840gtcatgctga gcttctggct cttgagcagc gccaactttg ggaacctgtg cgcggccttg 3900tggtcggccc atcgggcctc ttatgtgtca ttcttggcaa gttactcggt gggtcacgtt 3960atctctggca tgttctccta cgtctatgcc tgcttgcaga tttggccctt tctcttgttt 4020atgtggtgtc ccaggggcgt tgtcacaagt gttggggaaa gtgtataagg acagctcctg 4080ctgaggtggc ttttaatgta tttcctttct cgcgcgccac ccgtagttct cttgtatcct 4140tatgtgatcg attccaaacg ccaaaaggag ttgatcccgt gcacttggcg acaggttggc 4200gcgggtgctg gcgtggtgag agtcctatcc atcaaccaca ccaaaagccc atagcttacg 4260ccaatttgga tgaaaagaag atatctgccc aaacggttgt tgctgtccca tacgatccca 4320atcaggctat caaatgtctg aaagttctgc aggcgggagg ggctatcgtg gatcagccta 4380cgcctgaggt cgttcgtgtg tccgagatcc ccttctcagc cccatttttc ccaaaagttc 4440cagtcaaccc agattgtagg gtcgtggtgg attcggacac ttttgtggct gcggttcgtt 4500gtggttattc gacatcacaa ctggtcctgg gccagggcaa ctttgccaag ttaaatcaaa 4560ccccccccag gaactctatc tccaccaaag cgactggtgg ggcctcttat acttttgctg 4620tggctcaagt gtctgtgtgg acccttgttc atttcgtcct cggtctttgg ctcacgtcac 4680ctcaagtgtg tggtcgagga accgctgacc catggtgttc aagtccattt tcatatccta 4740cctatggccc cggggttgtg tgctcctctc gactttgtgt gtctgctgac ggggtcaccc 4800ttccattgtt ctcagccgtg gcacaactct ctggtaggga ggtggggatt tttattttag 4860tgctcgtctc ctttattgcc ttggcccacc gcatggctct taaggcagac atgttagtag 4920tctttttggc tctttgtgct tatgcctggc ccatgagctc ctggttgatc tgcttctttc 4980ctatactctt gaggtgggtt acccttcacc ctctcactat gctttgggtg cattcattct 5040tgatgttttg tctcccagca gccggcgtcc tctcactggg gataactggc ctcctctggg 5100caatcggccg ctttactcag gttgccggaa tcattacacc ttatgatatc caccagtaca 5160cctctgggcc acgtggtgca gccgctgtgg ccacagcccc agaaggcact tacatggccg 5220ccgtccggag agctgcttta accgggcgga ctttaatctt caccccgtcc gcagttggat 5280cccttctcga aggtgctttc aggactcata aaccctgcct taacaccgtg aatgtcgtag 5340gctcctccct tggttccgga ggggttttca ccattgacgg aaaaaaaatt gtcgtcactg 5400ctgcccatgt gctgaacggc gacacagcta gagtcaccgg tgattcctac aaccgcatgc 5460acactttcaa gactaatggt gactatgcct ggtcccatgc tgataactgg cagggcgctg 5520cccctgtggt caaggttgcg aaagggtatc gcggtcgtgc ctactggcaa acatcaactg 5580gtgtcgagcc tggtgttatt gggaatgggt tcgccttctg tttcaccaac tgcggcgatt 5640cggggtcacc cgttatctca gaatctggtg atcttatcgg aatccacacc ggttcaaaca 5700aacttggttc tggtcttgtg acaacccctg aaggggagac ctgtaccatc agagaaacca 5760agctttctga cctttccaga catttcgcag gcccaagcgt tcctcttggg gacatcaaat 5820tgagtccggc catcatccct gatgtgacat ccattccgag tgacttggca tcgctcctag 5880cttccgtccc tgtaatggaa ggcggcctct cgaccgttca acttttgtgt gtctttttcc 5940tcctctggcg catgatgggc catgcctgga cgcccattgt tgccgtgggc ttctttttgc 6000tgaatgaaat tcttccagca gttttggttc gagccgtgtt ttcttttgca ctctttgtgc 6060ttgcatgggc caccccctgg tctgcacaag tgttgatgat tagacttctc acggcatctc 6120tcaaccgcaa caaactttct cttgcgttct acgcactcgg aggtgttgtt ggtttggctg 6180ctgaaatcgg gacttttgct ggtaaattgt ctgaattgtc tcaagctctt tcgacatact 6240gtttcttacc tagggtcctt gctatgacca gctgtgttcc catcatcatc attggtggac 6300tccatgccct cggtgtgatt ctgtggttat tcaaataccg gtgcctccac aacatgctgg 6360ttggtgatgg aagcttttca agcgctttct tcctacggta ttttgcagag ggcaatctca 6420ggagaggtgt ttcacagtcc tgtggcatga gtaacgagtc cctgacggct gctttggctt 6480gcaagttgtc acaggctgac cttgattttt tgtccagctt aacgaacttc aagtgctttg 6540tatctgcttc aaacatgaaa aatgctgccg gccagtacat tgaagcagct tatgccaggg 6600ccctgcgtca agagttggcc tctttagtcc agattgacaa aatgaaagga gttttgtcca 6660agctagaggc ctttgctgaa acggccactc cgtccctcga cgtaggtgac gtgattgttc 6720tacttggaca acatcctcac ggatccgttc tcgatattaa tgtggggact gaaaggaaaa 6780ctgtatccgt gcaagagacc cggagcctag gcggctccaa gttcagtgtt tgtactgttg 6840tgtcaaacac acccgtggac gccttagccg gtattccact ccagacacca accccccttt 6900tcgagaatgg cccgcgtcat cgcagcgagg aggacgatct taaagtcgag aggatgaaga 6960aacactgcgt gtccctcggc ttccacaaca ttaacggtaa agtttactgc aagatttggg 7020acaagtctac cggtgacgcc ttttacactg atgattcccg gtacacccaa gactatgctt 7080ttcaggacag gtcagctgac tatagagaca gggactacga gggtgtgcaa accgcccccc 7140aacagggatt tgatccaaag tctgaaaccc ctgttggtac cgttgtgatc ggcggtatta 7200cgtacaacag gtatttggtc aaaggtaagg aggttctggt tcccaagcct gacaactgcc 7260ttgaagctgc caagctgtcc cttgagcaag ctctcgctgg gatgggccaa acttgtgacc 7320ttacagctgc cgaggtggaa aagctaaagc gcatcattgg tcaacttcaa ggattgacca 7380ctgagcaggc tttaaactgt tagccgccag cggcttgacc cgctgtggcc gcggcggcct 7440agttgtaact gaaacggcgg taaaaattgt caaataccac agcagaactt ttaccttagg 7500ctctttagac ctaaaagtca cttccgaggt ggaggtgaag aagtcaaccg agcagggcca 7560cgctgttgtg gcaaacttgt gttctggtgt cgtcttgatg agacctcacc caccgtccct 7620tgtcgacgtt cttctgaaac ccggacttga cataacaccc ggcattcaac cagggcatgg 7680ggccgggaat atgggcgtgg acggttccat ttgggatttt gaaaccgcac ccacaaaggc 7740tgaactcgag ttatccaagc aaataattca agcatgtgaa gtcaggcgcg gggatgcccc 7800gaacctccag ctcccttaca agctctatcc tgttagaggg gatcctgagc ggcataaagg 7860ccacctcatc aataccaggt ttggagactt accttacaaa actcctcaag acaccaagtc 7920cgcaatccac gcggcttgtt gcctgcaccc caacggggcc cccgtgtctg atggtaaatc 7980cacactaggt accactcttc aacatggctt cgagctttat gtccctactg tgccctatag 8040tgtcatggag taccttgatt cacgcccaga cacccctttt atgtgcacta aacacggcac 8100ttccaaggct gctgcagagg acctccaaaa atacgaccta tccacccaag gatttgtcct 8160gcctggggtc ctacgcctag tgcgcaggtt catctttggc cacattggca aggcaccgcc 8220attgttcctc ccatcaactt atcccgccaa gaactccatg gcaggtatta atggtcagag 8280gttcccaaca aaggatgttc aaagtatacc tgaaattgat gaaatgtgtg cccgcgccgt 8340caaggagaat tggcaaactg tgacaccttg caccctcaag aaacagtatt gttctaggcc 8400caaaaccagg accatcctgg gcactaacaa cttcatagcc ttggctcata gatcggcgct 8460cagtggtgtt acccaggcat tcatgaagaa ggcttggaag tccccaatag ccttagggaa 8520aaacaaattc aaggagctgc attgcactgt cgctggcagg tgcctcgagg ccgacttggc 8580ttcctgtgac cgcagcaccc ctgccattgt gaggtggttc actacccacc tcctatatga 8640acttgcagga tgtgaagaat atctacctag ctatgtgctt aactgttgcc atgaccttgt 8700ggcgacgcag gatggtgctt tcacaaaacg cggtggcctg tcgtctggag acccagtcac 8760cagtgtgtcc aacactgtgt actcactggt gatttatgcc cagcacatgg tactatctgc 8820cctgaaaatg ggtcatgaaa ttggcctcaa gttcctcgaa gaacaactca aatttgagga 8880ccttcttgaa atccagccta tgttagtata ctctgatgat cttgtcttgt acgcagaaaa 8940gcccaccttc cccaactatc attggtgggt cgagcatctt gacctgatgt tgggctttaa 9000aacggaccca aagaaaaccg tcataactga taaacccagt ttcctcgggt gcagaatcga 9060agcagggcga cagctagtcc ccaatcgcga ccgcatcctg gctgctcttg catatcacat 9120gaaggcgcag aacgcctcag agtattatgc gtctgctgcc gcaatcctga tggattcatg 9180tgcttgcatt gaccacgatc ctgaatggta tgaggacctc atctgtggca ttgcccgatg 9240cgctcgcctg gacggttata gctttccagg tccggcattt ttcatgtcca tgtgggagaa 9300gctgaggagt cataatgaag ggaagaaatt ccgccactgc ggcatctgcg acgccaaagc 9360cgactacgcg gctgcttgtg ggcttgattt gtgtttgttt cattcgcact ttcaccaaca 9420ctgccctgtc actctgagct gcggtcacca tgccggttcg aaggaatgtt cgcagtgtca 9480gtcacctgtt ggggccggca gatcccctct tgatgctgtg ctggaacaaa ttccatacaa 9540acctcctcgc actgtcatca tgaaggtggg taataaaaca acggcccttg atccggggag 9600gtaccagtcc cgtcgaggtc tcgttgcagt caagaggggt attgcgggta atgaagttga 9660tcttgctgat ggagattacc aagtggtacc tcttctgccg acttgcaaag atataaacat 9720ggtgaaggtg gcttgcaacg tactactcag caagttcata gtagggccac caggttccgg 9780gaagaccacc tggctactaa gtcaagttca ggacgatgat gtcatttaca cacccactca 9840tcagaccatg tttgacatag ttagtgctct caaagtttgc aggtattcca ttccaggagc 9900ctcgggactc cctttcccac cgcctgccag gtccgggccg tgggttaggc tcattgccag 9960cgggcacgtc cctggccgag tatcatacct cgatgaggcc ggatattgca atcatctgga 10020cattcttaga ctgctttcca aaacacccct tgtgtgtttg ggtgaccttc agcaacttca 10080cccagtcggc tttgattcct attgttatgt gttcgatcag atgcctcaga agcagttgac 10140caccatttac agatttggcc ccaacatctg cgcagccatc cagccttgtt acagggagaa 10200acttgaatct aaggctagga acaccagggt ggtttttacc acccggcctg tggcctttgg 10260ccaggtgctg acaccatatc acaaagatcg cgtcggctcc gcgattacca tagactcatc 10320ccagggggcc acctttgaca ttgtaacatt gcatctacca tcgccaaagt ccctaaataa 10380gtcccgggca cttgtggcca tcacacgggc aagacacggg ttgttcattt atgaccctca 10440caaccagctc cgggagtttt tcaacctaac ccctgagcgc actgattgta accttgtgtt 10500cagccgtgga gatgagctgg tggtcctgaa tgcagataat gcagtcacaa ccgtggcgaa 10560ggccttagag acaggtccaa ctcaatttcg agtgtcagac ccgaggtgca agtctctctt 10620agccgcttgc tcggccagtc tggaagggag ctgcatgccg ctaccgcaag tggcgcataa 10680cctggggttt tacttctccc cagacagtcc agtatttgca cctctgccaa aagagttggc 10740gccacattgg ccagtggtta cccatcagaa taatcgggcg tggcctgatc gacttgtcgc 10800tagtatgcgt ccaattgacg cccgctacag caagccgatg gtcggtgcag ggtatgtggt 10860cggaccgtcc accttccttg gtactcctgg agtggtgtca tactatctca cactatacat 10920caggggtgag ccccaggcct tgccagaaac acttgtttca acaggacgta tagccacaga 10980ttgtcgggag tatctcgacg cggctgagga agaggcagca aaagaacttc cccacgcgtt 11040cattggcgat gtcaaaggca ccacagttgg ggggtgtcat cacattacat caaaatacct 11100acctaggtcc ctgcctaaag actctgttgc cgtagttgga gtaagttcgc ctggcagggc 11160tgctaaagcc gtatgcaccc tcaccgatgt gtacctccct gaactccggc catatctgca 11220acctgagacg gcatcaaaat gctggaaact caaattagac ttcagggacg tccgactaat 11280ggtctggaaa ggagccaccg cctactttca attggaaggg ctcacatggt cggcgctgcc 11340tgactatgcc aggtttattc agctgcccaa aaacgctgtt gtatacatcg atccgtgcat 11400aggaccggca acagccaatc gtaaagtcgt acgaaccaca gattggcggg ccgacctggc 11460agtgacgccg tatgattacg gtgcccggaa cattttgaca acagcctggt tcgaggacct 11520cgggccgcag tggaagattc tggggttgca gccctttagg cgggcgtttg gctttgaaaa 11580cactgaggat tgggcaatcc ttgcatgctg catgagtgac ggcaaggact acactgacta 11640taactggaat tgcgttcgac aacgcccaca cgctatccat ggacgcgctc gtgaccatac 11700gtaccacttt gcccctggca ctgaattgca agtggagctc ggtaaacccc ggctgccacc 11760tgagcaagta ccgtgaattc ggagtgatgc aatggggtca ctgtggagta aaatcagcca 11820gctgttcgtg gacgccttca ctgaattcct tgttagtgtg gttgatattg tcatcttcct 11880tgccatattg tttgggttca ccgtcgcagg atggttactg gtctttcttc tcagagtggt 11940ttgctccgcg cttctccgtt cgcgctctgc cattcactct tccgaactat cgaaggtcct 12000atgagggctt actacctaat tgcagaccgg atgttccaca atttgcattt aagcaccctt 12060tgggtatgtt ttggcacatg cgggtttccc acctaattga tcagatggtc tctcgccgca 12120tctaccagac catggaacat tcaggtcaag cggcctggaa gcacgtggtc agtgaggcta 12180ctcttacaaa attgtcagaa ctcgacatag ttctccactt ccaacacctg gccgcagtgg 12240aggcggactc ttgtcgcttc ctcagctcac gacttgtgat gctgaaaaat cttgccgttg 12300gcaatgtgag cttgcagtac aacaccacgt tgaaccgcgt tgagctcatc ctccccacac 12360caggtacgag gcccaaattg accgatttca gacaatggct catcagtgtg cacgcttcca 12420ttttttcctc tgtagcctca tcagttactt tgttcatagt gctttggctt cgaattccag 12480ccgtacgcta tgtttttggt ttccattggc ccatggcaac acgtcattcg agctgaccat 12540taattacact atatgcatgc cctgtcttac cagccaagcg gctcaacaaa ggctcgaacc 12600cggtcgtaac atgtggtgca aaataggaca caccacgtgc gaggagcgtg accatgatga 12660gttgtcaatg tccatcccgt ccgggtacga caacctcaaa cttgaaggtt attacgcttg 12720gctggctttt ttgtcctttt cctacgcggc ccaattccat ccggagttgt ttggaatagg 12780gaatgtgtcg cgcgtctttg tggataaacg acaccagttc atttgtgccg agcatgacgg 12840agataattca accgtatcta ccggacacaa catctccgca tcatatgcgg catattatca 12900ccaccaaata gacgggggca attggttcca tttggaatgg ctgcggccgc tcttttcctc 12960ttggctagtg ctcaacatat catggtttct gaggcgttcg cctgcaagcc ctgtttctcg 13020acgcatctat cagatattaa gaccaatacg accgcggctg ccggtttcat ggtccttcaa 13080gacatcagtt gcctccgacc tcacagggtc tcagcatcgc aagagaacat tcccttcgga 13140aagtcgtcac aatgtcgtga agccgtcggt actccccagt acattacgat gactgctaat 13200gtgaccgacg aatcatattt gtacaacgcg gacttgctaa tgctttccgc gtgccttttc 13260cacgcctcag aaatgagcga gaaaggcttc aaagttatct ttggaaacgt ctccggcgtt 13320gtttcagctt gtgtcaattt cacagattat gtggcccatg taacccaaca tacccaacag 13380catcatctgg taattgatca cattcggtta ctgcatttcc tgacaccatc tgcaatgagg 13440tgggctacaa ccattgcttg tttgttcgcc attctcttag cgatatgaga tgttctcaca 13500aattggggcg tttcttgact ccgcactctt gcttctggtg gctttttttg ctgtgtaccg 13560gcttgtcctg gtcctttgcc gatggcaacg gcaacagctc gacacgccaa tacatatata 13620acttgacgat atgcgagctg aatgggaccg tctggttgtc cagtcatttt gattgggcag 13680tcgagacctt tgtgctttac ccggtggcca ctcatatcct ctcactgggt tttctcacaa 13740caagccattt ttttgatgcg ctcggtctcg gcgctgtgtc cactacggga tttcttggcg 13800ggcggtatgt acttagcagc gtgtacggcg cctgcgcctt cgcagcgctt gtatgttttg 13860tcatccgtgc tgctaaaaat tgcatggctt gccgttatgc ccgcacccgg ttcaccaact 13920tcatcgtgga cgaccggggg aagatccatc gatggaagtc cccaatagtg gtagagaaat 13980taggcaaagc tgacatcggc ggcgaccttg tcaccatcaa acatgttgtc ctcgaaggag 14040tcaaagctca acctttgacg aggacatcgg cggagcaatg ggaagcctag atgatttttg 14100caatgatcct accgccgcac agaagcttgt gctggcattt agtatcacat acacacctat 14160aatgatatac gccctcaagg tgtcacgcgg ccggctccta ggactgttac acatcctgat 14220atttctgaac tgttctttca cgttcggata catgacatac gtgcactttc aatccactaa 14280ccgtgtcgcg cttactatgg gggcggtcgt tgcccttttg tggggcattt acagctttat 14340agaatcatgg aagtttgtca cttccagatg caggttgtgt tgcctaggcc ggcgatacat 14400tctggcccct gcccaccacg tagaaagtgc tgcaggcctc cattcaatcc cagcgtctgg 14460taaccgagca tacgctgtga gaaagcccgg actaacatca gtgaacggca ctctagtacc 14520aggacttcgg agcctcgtgt tgggcggcaa acgagctgtt

aaacgaggag tggttaacct 14580cgtcaagtat ggccggtaaa aaccagagcc agaagaaaaa gaaaaacaca gctcctatgg 14640ggagtggcca gccagtcaat caactgtgcc aattgctggg cacaatgata aagtcccagc 14700gccagcggcc taggggagga caggccaaaa tgaaaaagcc tgagaagcca catttccccc 14760tagctgctga agatgacatc cggcaccatt tcacccagac cgagcgttcc ctttgcttgc 14820aatcgatcca gacggccttc aatcaaggcg caggaactgc gtcgctttca tccagcggga 14880aggtcagttt tcaggttgag ttcatgctgc cggtcgctca tacggtgcgc ctgattcgcg 14940taacttccac atccgccagt cagggtgcaa gttaatttga tagttaggtg aatggccgcg 15000attggcgtgt ggcctctgag tcacctattc aattagggcg atcacatggg ggttagactt 15060aattggcgag aaccatgtga ccgaaattaa aaaaaaaaaa aaaaaaaaaa aaaaaa 151161415111DNAPorcine reproductive and respiratory syndrome virus 14atgatgtgta gggtattccc cctacataca cgacacttct agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgcgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180tccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gcccgggtat tttggaacgc cggccaagtc ttttgcacac 300ggtgtctcag tgcgcggtct cttctctctc cagagcttca ggacactgac ctcggtgcag 360ttggcttgtt ttacaagcct agggacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggaatg tactccatcc gggtgctgtt ggctctcagc tgttttccct ttggcgcgta 480tgacctccgg caatcacaac ttcctccaac gacttgtgaa ggttgctgat gttttgtacc 540gtgacggttg cttggcacct cgacaccttc gtgaactcca agtttacgag cgcggctgca 600actggtaccc gatcacgggg cccgtgcccg ggatgggttt gtttgcgaac tccatgcacg 660tatccgacca gccgttccct ggtgccaccc atgtgttgac taactcgcct ttgcctcaac 720aggcttgtcg gcagccgttc tgtccatttg aggaggctca ttctagcgtg tacaggtgga 780agaaatttgt ggttttcacg gactcctccc tcaacggtcg atctcgcatg atgtggacgc 840cggaatccga tgattcagcc gccctggagg tactaccgcc tgagttagaa cgtcaggtcg 900aaatcctcat tcggagtttt cctgctcatc accctgtcga cctggccgac tgggagctca 960ctgagtcccc tgagaacggt ttttccttca acacgtctca ttcttgcggt caccttgtcc 1020agaaccccga cgtgtttgat ggcaagtgct ggctctcctg ctttttgggc cagtcggtcg 1080aagtgcgctg ccatgaggaa catctagctg acgccttcgg ttaccaaacc aagtggggcg 1140tgcatggtaa gtacctccag cgcaggcttc aagttcgcgg cattcgtgct gtagtcgatc 1200ctgatggtcc cattcacgtt gaagcgctgt cttgccccca gtcttggatc aggcacctga 1260ctctggatga tgatgtcacc ccaggattcg ttcgcctgac atcccttcgc attgtgccga 1320acacagagcc taccacttcc cggatctttc ggtttggagc gcataagtgg tatggcgctg 1380ccggcaaacg ggctcgtgct aagcgtgccg ctaaaagtga gaaggattcg gctcccaccc 1440ccaaggttgc cctgccggtc cccacctgtg gaattaccac ctactctcca ccgacagacg 1500ggtcttgtgg ttggcatgtc cttgccgcca taatgaaccg gatgataaat ggtgacttca 1560cgtcccctct gactcagtac aacagaccag aggatgattg ggcttctgat tatgatcttg 1620ttcaggcgat tcaatgtcta cgactgcctg ctaccgtggt tcggaatcgc gcctgtccta 1680acgccaagta ccttataaaa cttaacggag ttcactggga ggtagaggtg aggtctggaa 1740tggctcctcg ctccctttct cgtgaatgtg tggttggcgt ttgctctgaa ggctgtgtcg 1800caccgcctta tccagcagac gggctaccta aacgtgcact cgaggccttg gcgtctgctt 1860acagactacc ctccgattgt gttagctctg gtattgctga ctttcttgct aatccacctc 1920ctcaggaatt ctggaccctc gacaaaatgt tgacctcccc gtcaccagag cggtccggct 1980tctctagttt gtataaatta ctattagagg ttgttccgca aaaatgcggt gccacggaag 2040gggctttcat ctatgctgtt gagaggatgt tgaaggattg tccgagctcc aaacaggcca 2100tggcccttct ggcaaaaatt aaagttccat cctcaaaggc cccgtctgtg tccctggacg 2160agtgtttccc tacggatgtt ttagccgact tcgagccagc atctcaggaa aggccccaaa 2220gttccggcgc tgctgttgtc ctgtgttcac cggatgcaaa agagttcgag gaagcagccc 2280cggaagaagt tcaagagagt ggccacaagg ccgtccactc tgcactcctt gccgagggtc 2340ctaacaatga gcaggtacag gtggttgccg gtgagcaact gaagctcggc ggttgtggtt 2400tggcagtcgg gaatgctcat gaaggtgctc tggtctcagc tggtctaatt aacctggtag 2460gcgggaattt gtccccctca gaccccatga aagaaaacat gctcaatagc cgggaagacg 2520aaccactgga tttgtcccaa ccagcaccag cttccacaac gacccttgtg agagagcaaa 2580cacccgacaa cccaggttct gatgccggtg ccctccccgt caccgttcga gaatttgtcc 2640cgacggggcc tatactctgt catgttgagc actgcggcac ggagtcgggc gacagcagtt 2700cgcctttgga tctatctgat gcgcaaaccc tggaccagcc tttaaatcta tccctggccg 2760cttggccagt gagggccacc gcgtctgacc ctggctgggt ccacggtagg cgcgagcctg 2820tctttgtaaa gcctcgaaat gctttctctg atggcgattc agcccttcag ttcggggagc 2880tttctgaatc cagctctgtc atcgagtttg accggacaaa agatgctccg gtggttgacg 2940cccctgtcga cttgacgact tcgaacgagg ccctctctgt agtcgatcct ttcgaatttg 3000ccgaactcaa gcgcccgcgt ttctccgcac aagccttaat tgaccgaggc ggtccacttg 3060ccgatgtcca tgcaaaaata aagaaccggg tatatgaaca gtgcctccaa gcttgtgagc 3120ccggtagtcg tgcaacccca gccaccaggg agtggctcga caaaatgtgg gatagggtgg 3180acatgaaaac ttggcgctgc acctcgcagt tccaagctgg tcgcattctt gcgtccctca 3240aattcctccc tgacatgatt caagacacac cgcctcctgt tcccaggaag aaccgagcta 3300gtgacaatgc cggcctgaag caactggtgg cacagtggga taggaaattg agtgtgaccc 3360cccccccaaa accggttggg ccagtgcttg accagatcgt ccctccgcct acggatatcc 3420agcaagaaga tgtcaccccc tccgatgggc caccccatgc gccggatttt cctagtcgag 3480tgagcacggg cgggagttgg aaaggcctta tgctttccgg cacccgtctc gcggggtcta 3540tcagccagcg ccttatgaca tgggtttttg aagttttctc ccacctccca gcttttatgc 3600tcacactttt ctcgccgcgg ggctctatgg ctccaggtga ttggttgttt gcaggtgtcg 3660ttttacttgc tctcttgctc tgtcgttctt acccgatact cggatgcctt cccttattgg 3720gtgtcttttc tggttctttg cggcgtgttc gtctgggtgt ttttggttct tggatggctt 3780ttgctgtatt tttattctcg actccatcca acccagtcgg ttcttcttgt gaccacgatt 3840cgccggagtg tcatgctgag cttttggctc ttgagcagcg ccaactttgg gaacctgtgc 3900gcggccttgt ggtcggcccc tcaggcctct tatgtgtcat tcttggcaag ttactcggtg 3960ggtcacgtta tctctggcat gttctcctac gtttatgcat gcttgcagat ttggcccttt 4020ctcttgttta tgtggtgtcc caggggcgtt gtcacaagtg ttggggaaag tgtataagga 4080cagctcctgc ggaggtggct cttaatgtat ttcctttctc gcgcgccacc cgtgtctctc 4140ttgtatcctt gtgtgatcga ttccaaacgc caaaaggggt tgatcctgtg cacttggcaa 4200cgggttggcg cgggtgctgg cgtggtgaga gccccatcca tcaaccacac caaaagccca 4260tagcttatgc caatttggat gaaaagaaaa tgtctgccca aacggtggtt gctgtcccat 4320acgatcccag tcaggctatc aaatgcctga aagttctgca ggcgggaggg gccatcgtgg 4380accagcctac acctgaggtc gttcgtgtgt ccgagatccc cttctcagcc ccatttttcc 4440caaaagttcc agtcaaccca gattgcaggg ttgtggtaga ttcggacact tttgtggctg 4500cggttcgctg cggttactcg acagcacaac tggttctggg ccggggcaac tttgccaagt 4560taaatcagac cccccccagg aactctatct ccaccaaaac gactggtggg gcctcttaca 4620cccttgctgt ggctcaagtg tctgcgtgga ctcttgttca tttcatcctc ggtctttggt 4680tcacatcacc tcaagtgtgt ggccgaggaa ccgctgaccc atggtgttca aatccttttt 4740catatcctac ctatggcccc ggagttgtgt gctcctctcg actttgtgtg tctgccgacg 4800gggtcaccct gccattgttc tcagccgtgg cacaactctc cggtagagag gtggggattt 4860ttattttggt gctcgtctcc ttgactgctt tggcccaccg catggctctt aaggcagaca 4920tgttagtggt cttttcggct ttttgtgctt acgcctggcc catgagctcc tggttaatct 4980gcttctttcc tatactcttg aagtgggtta cccttcaccc tcttactatg ctttgggtgc 5040actcattctt ggtgttttgt ctgccagcag ccggcatcct ctcactaggg ataactggcc 5100ttctttgggc aattggccgc tttacccagg ttgccggaat tattacacct tatgacatcc 5160accagtacac ctctgggcca cgtggtgcag ctgctgtggc cacagcccca gaaggcactt 5220atatggccgc cgtccggaga gctgctttaa ctgggcgaac tttaatcttc accccgtctg 5280cagttggatc ccttctcgaa ggtgctttca ggactcataa accctgcctt aacaccgtga 5340atgttgtagg ctcttccctt ggttccggag gggttttcac cattgatggc agaagaactg 5400tcgtcactgc tgcccatgtg ttgaacggcg acacagctag agtcaccggc gactcctaca 5460accgcatgca cactttcaag accaatggtg attatgcctg gtcccatgct gatgactggc 5520agggcgttgc ccctgtggtc aaggttgcga aggggtaccg cggtcgtgcc tactggcaaa 5580catcaactgg tgtcgaaccc ggtatcattg gggaagggtt cgccttctgt tttactaact 5640gcggcgattc ggggtcaccc gtcatctcag aatctggtga tcttattgga atccacaccg 5700gttcaaacaa acttggttct ggtcttgtga caacccctga aggggagacc tgcaccatca 5760aagaaaccaa gctctctgac ctttccagac attttgcagg cccaagcgtt cctcttgggg 5820acattaaatt gagtccggcc atcatccctg atgtaacatc cattccgagt gacttggcat 5880cgctcctagc ctccgtccct gtagtggaag gcggcctctc gaccgttcaa cttttgtgtg 5940tctttttcct tctctggcgc atgatgggcc atgcctggac acccattgtt gccgtgggct 6000tctttttgct gaatgaaatt cttccagcag ttttggtccg agccgtgttt tcttttgcac 6060tctttgtgct tgcatgggcc accccctggt ctgcacaggt gttgatgatt agactcctca 6120cggcatctct caaccgcaac aagctttctc tggcgttcta cgcactcggg ggtgtcgtcg 6180gtttggcagc tgaaatcggg acttttgctg gcagattgtc tgaattgtct caagctcttt 6240cgacatactg cttcttacct agggtccttg ctatgaccag ttgtgttccc accatcatca 6300ttggtggact ccataccctc ggtgtgattc tgtggttatt caaataccgg tgcctccaca 6360acatgctggt tggtgatggg agtttttcaa gcgccttctt cctacggtat tttgcagagg 6420gtaatctcag aaaaggtgtt tcacagtcct gtggcatgaa taacgagtcc ctaacggctg 6480ctttagcttg caagttgtca caggctgacc ttgatttttt gtccagctta acgaacttca 6540agtgctttgt atctgcttca aacatgaaaa atgctgccgg ccagtacatt gaagcagcgt 6600atgccaaggc cctgcgccaa gagttggcct ctctagttca gattgacaaa atgaaaggag 6660ttttgtccaa gctcgaggcc tttgctgaaa cagccacccc gtcccttgac ataggtgacg 6720tgattgttct gcttgggcaa catcctcacg gatccatcct cgatattaat gtggggactg 6780aaaggaaaac tgtgtccgtg caagagaccc ggagcctagg cggctccaaa ttcagtgttt 6840gtactgtcgt gtccaacaca cccgtggacg ccttgaccgg catcccactc cagacaccaa 6900cccctctttt tgagaatggt ccgcgtcatc gcagcgagga agacgatctt aaagtcgaga 6960ggatgaagaa acactgtgta tccctcggct tccacaacat caatggcaaa gtttactgca 7020aaatttggga caagtctacc ggtgacacct tttacacgga tgattcccgg tacacccaag 7080accatgcttt tcaggacagg tcagccgact acagagacag ggactatgag ggtgtgcaaa 7140ccacccccca acagggattt gatccaaagt ctgaaacccc tgttggcact gttgtgatcg 7200gcggtattac gtataacagg tatctgatca aaggtaagga ggttctggtc cccaagcctg 7260acaactgcct tgaagctgcc aagctgtccc ttgagcaagc tctcgctggg atgggccaaa 7320cttgcgacct tacagctgcc gaggtggaaa agctaaagcg catcattagt caactccaag 7380gtttgaccac tgaacaggct ttaaactgtt agccgccagc ggcttgaccc gctgtggccg 7440cggcggccta gttgtgactg aaacggcggt aaaaattata aaataccaca gcagaacttt 7500caccttaggc cctttagacc taaaagtcac ttccgaggtg gaggtaaaga aatcaactga 7560gcagggccac gctgttgtgg caaacttatg ttccggtgtc atcttgatga gacctcaccc 7620accgtccctt gtcgacgttc ttctgaaacc cggacttgac acaatacccg gcattcaacc 7680agggcatggg gccgggaata tgggcgtgga cggttctatt tgggattttg aaaccgcacc 7740cacaaaggca gaactcgagt tatccaagca aataatccaa gcatgtgaag ttaggcgcgg 7800ggacgccccg aacctccaac tcccttacaa gctctatcct gttagggggg atcctgagcg 7860gcataaaggc cgccttatca ataccaggtt tggagattta ccttacaaaa ctcctcaaga 7920caccaagtcc gcaatccacg cggcttgttg cctgcacccc aacggggccc ccgtgtctga 7980tggtaaatcc acactaggta ccactcttca acatggtttc gagctttatg tccctactgt 8040gccctatagt gtcatggagt accttgattc acgccctgac acccctttta tgtgtactaa 8100acatggcact tccaaggctg ctgcagagga cctccaaaaa tacgacctat ccacccaagg 8160atttgtcctg cctggggtcc tacgcctagt acgcagattc atctttggcc atattggtaa 8220ggcgccgcca ttgttcctcc catcaaccta tcccgccaag aactctatgg cagggatcaa 8280tggccagagg ttcccaacaa aggacgttca gagcatacct gaaattgatg aaatgtgtgc 8340ccgcgctgtc aaggagaatt ggcaaactgt gacaccttgc accctcaaga aacagtactg 8400ttccaagccc aaaaccagga ccatcctggg caccaacaac tttattgcct tggctcacag 8460atcggcgctc agtggtgtca cccaggcatt catgaagaag gcttggaagt ccccaattgc 8520cttggggaaa aacaaattca aggagctgca ttgcactgtc gccggcaggt gtcttgaggc 8580cgacttggcc tcctgtgacc gcagcacccc cgccattgta agatggtttg ttgccaacct 8640cctgtatgaa cttgcaggat gtgaagagta cttgcctagc tatgtgctta attgctgcca 8700tgacctcgtg gcaacacagg atggtgcctt cacaaaacgc ggtggcctgt cgtccgggga 8760ccccgtcacc agtgtgtcca acaccgtata ttcactggta atttatgccc agcacatggt 8820attgtcggcc ttgaaaatgg gtcatgaaat tggtcttaag ttcctcgagg aacagctcaa 8880gttcgaggac ctccttgaaa ttcagcctat gttggtatac tctgatgatc ttgtcttgta 8940cgctgaaaga cccacatttc ccaattacca ctggtgggtc gagcaccttg acctgatgct 9000gggtttcaga acggacccaa agaaaaccgt cataactgat aaacccagct tcctcggctg 9060cagaattgag gcagggcgac agctagtccc caatcgcgac cgcatcctgg ctgctcttgc 9120atatcacatg aaggcgcaga acgcctcaga gtattatgcg tctgctgccg caatcctgat 9180ggattcatgt gcttgcattg accatgaccc tgagtggtat gaggacctca tctgcggtat 9240tgcccggtgc gcccgccagg atggttatag cttcccaggt ccggcatttt tcatgtccat 9300gtgggagaag ctgagaagtc ataatgaagg gaagaaattc cgccactgcg gcatctgcga 9360cgccaaagcc gactatgcgt ccgcctgtgg gcttgatttg tgtttgttcc attcgcactt 9420tcatcaacac tgccctgtca ctctgagctg cggtcaccat gccggttcaa aggaatgttc 9480gcagtgtcag tcacctgttg gggctggcag atcccctctt gatgccgtgc taaaacaaat 9540tccatacaaa cctcctcgta ctgtcatcat gaaggtgggt aataaaacaa cggccctcga 9600tccggggagg taccagtccc gtcgaggtct cgttgcagtc aagaggggta ttgcaggcaa 9660tgaagttgat ctttctgatg gggactacca agtggtgcct cttttgccga cttgcaaaga 9720cataaacatg gtgaaggtgg cttgcaatgt actactcagc aagttcatag tagggccacc 9780aggttccgga aagaccacct ggctactgag tcaagtccag gacgatgatg tcatttacac 9840acccacccat cagactatgt ttgatatagt cagtgctctc aaagtttgca ggtattccat 9900tccaggagcc tcaggactcc ctttcccacc acctgccagg tccgggccgt gggttaggct 9960tattgccagc gggcacgtcc ctggccgagt atcatacctc gatgaggctg gatattgtaa 10020tcatctggac attcttagac tgctttccaa aacacccctt gtgtgtttgg gtgaccttca 10080gcaacttcac cctgtcggct ttgattccta ctgttatgtg ttcgatcaga tgcctcagaa 10140gcagctgacc actatttaca gatttggccc taacatctgc gcagccatcc agccttgtta 10200cagggagaaa cttgaatcta aggctaggaa cactagggtg gtttttacca cccggcctgt 10260ggcctttggt caggtgctga caccatacca taaagatcgc atcggctctg cgataaccat 10320agattcatcc cagggggcca cctttgatat tgtgacattg catctaccat cgccaaagtc 10380cctaaataaa tcccgagcac ttgtagccat cactcgggca agacacgggt tgttcattta 10440tgaccctcat aaccagctcc aggagttttt caacttaacc cctgagcgca ctgattgtaa 10500ccttgtgttc agccgtgggg atgagctggt agttctgaat gcggataatg cagtcacaac 10560tgtagcgaag gcccttgaga caggtccatc tcgatttcga gtatcagacc cgaggtgcaa 10620gtctctctta gccgcttgtt cggccagtct ggaagggagc tgtatgccac taccgcaagt 10680ggcacataac ctggggtttt acttttcccc ggacagtcca acatttgcac ctctgccaaa 10740agagttggcg ccacattggc cagtggttac ccaccagaat aatcgggcgt ggcctgatcg 10800acttgtcgct agtatgcgcc caattgatgc ccgctacagc aagccaatgg tcggtgcagg 10860gtatgtggtc gggccgtcca cctttcttgg tactcctggt gtggtgtcat actatctcac 10920actatacatc aggggtgagc cccaggcctt gccagaaaca ctcgtttcaa cagggcgtat 10980agccacagat tgtcgggagt atctcgacgc ggctgaggaa gaggcagcaa aagaactccc 11040ccacgcattc attggcgatg tcaaaggtac cacggttggg gggtgtcatc acattacatc 11100aaaataccta cctaggtccc tgcctaagga ctctgttgcc gtagttggag taagttcgcc 11160cggcagggct gctaaagccg tgtgcactct caccgatgtg tacctccccg aactccggcc 11220atatctgcaa cctgagacgg catcaaaatg ctggaaactc aaattagact tcagggacgt 11280ccgactaatg gtctggaaag gagccaccgc ctatttccag ttggaagggc ttacatggtc 11340ggcgctgccc gactatgcca ggtttattca gctgcccaag gatgccgttg tatacattga 11400tccgtgtata ggaccggcaa cagccaaccg taaggtcgtg cgaaccacag actggcgggc 11460cgacctggca gtgacaccgt atgattacgg tgcccagaac attttgacaa cagcctggtt 11520cgaggacctc gggccgcagt ggaagatttt ggggttgcag ccctttaggc gagcatttgg 11580ctttgaaaac actgaggatt gggcaatcct tgcacgccgt atgaatgacg gcaaggacta 11640cactgactat aactggaact gtgttcgaga acgcccacac gccatctacg ggcgtgctcg 11700tgaccatacg tatcattttg cccctggcac agaattgcag gtagagctag gtaaaccccg 11760gctgccgcct gggcaagtgc cgtgaattcg gggtgatgca atggggtcac tgtggagtaa 11820aatcagccag ctgttcgtgg acgccttcac tgagttcctt gttagtgtgg ttgatattgc 11880cattttcctt gccatactgt ttgggttcac cgtcgcagga tggttactgg tctttcttct 11940cagagtggtt tgctccgcgc ttctccgttc gcgctctgcc attcactctc ccgaactatc 12000gaaggtccta tgaaggcttg ttgcccaact gcagaccgga tgtcccacaa tttgcagtca 12060agcacccatt gggtatgttt tggcacatgc gagtttccca cttgattgat gagatggtct 12120ctcgtcgcat ttaccagacc atggaacatt caggtcaagc ggcctggaag caggtggttg 12180gtgaggccac tctcacgaag ctgtcagggc tcgatatagt tactcatttc caacacctgg 12240ccgcagtgga ggcggattct tgccgctttc tcagctcacg actcgtgatg ctaaaaaatc 12300ttgccgttgg caatgtgagc ctacagtaca acaccacgtt ggaccgcgtt gagctcatct 12360tccccacgcc aggtacgagg cccaagttga ccgatttcag acaatggctc atcagtgtgc 12420acgcttccat tttttcctct gtggcttcat ctgttacctt gttcatagtg ctttggcttc 12480gaattccagc tctacgctat gtttttggtt tccattggcc cacggcaaca catcattcga 12540gctgaccatc aactacacca tatgcatgcc ctgttctacc agtcaagcgg ctcgccaaag 12600gctcgagccc ggtcgtaaca tgtggtgcaa aatagggcat gacaggtgtg aggagcgtga 12660ccatgatgag ttgttaatgt ccatcccgtc cgggtacgac aacctcaaac ttgagggtta 12720ttatgcttgg ctggcttttt tgtccttttc ctacgcggcc caattccatc cggagttgtt 12780cgggataggg aatgtgtcgc gcgtcttcgt ggacaagcga caccagttca tttgtgccga 12840gcatgatgga cacaattcaa ccgtatctac cggacacaac atctccgcat tatatgcggc 12900atattaccac caccaaatag acgggggcaa ttggttccat ttggaatggc tgcggccact 12960cttttcttcc tggctggtgc tcaacatatc atggtttctg aggcgttcgc ctgtaagccc 13020tgtttctcga cgcatctatc agatattgag accaacacga ccgcggctgc cggtttcatg 13080gtccttcagg acatcaattg tttccgacct cacggggtct cagcagcgca agagaaaatt 13140tccttcggaa agtcgtccca atgtcgtgaa gccgtcggta ctccccagta catcacgata 13200acggctaacg tgaccgacga atcatacttg tacaacgcgg acctgctgat gctttctgcg 13260tgccttttct acgcctcaga aatgagcgag aaaggcttca aagtcatctt tgggaatgtc 13320tctggcgttg tttctgcttg tgtcaatttc acagattatg tggcccatgt gacccaacat 13380acccagcagc atcatctggt aattgatcac attcggttgc tgcatttcct gacaccatct 13440gcaatgaggt gggctacaac cattgcttgt ttgttcgcca ttctcttggc aatatgagat 13500gttctcacaa attggggcgt ttcttgactc cgcactcttg cttctggtgg ctttttttgc 13560tgtgtaccgg cttgtcctgg tcctttgccg atggcaacgg cgacagctcg acataccaat 13620acatatataa cttgacgata tgcgagctga atgggaccga ctggttgtcc agccattttg 13680gttgggcagt cgagaccttt gtgctttacc cggttgccac tcatatcctc tcactgggtt 13740ttctcacaac aagccatttt tttgacgcgc tcggtctcgg cgctgtatcc actgcaggat 13800ttgttggcgg gcggtacgta ctctgcagcg tctacggcgc ttgtgctttc gcagcgttcg 13860tatgttttgt catccgtgct gctaaaaatt gcatggcctg ccgctatgcc cgtacccggt 13920ttaccaactt cattgtggac gaccggggga gagttcatcg atggaagtct ccaatagtgg 13980tagaaaaatt gggcaaagcc gaagtcgatg gcaacctcgt caccatcaaa catgtcgtcc 14040tcgaaggggt taaagctcaa cccttgacga ggacttcggc tgagcaatgg gaggcctaga 14100cgatttttgc aacgatccta tcgccgcaca aaagctcgtg ctagccttta gcatcacata 14160cacacctata atgatatacg cccttaaggt gtcacgcggc cgactcctgg ggctgttgca 14220catcctaata tttctgaact gttcctttac attcggatac atgacatatg tgcattttca 14280atccaccaac cgtgtcgcac ttaccctggg ggctgttgtc gcccttctgt ggggtgttta 14340cagcttcaca gagtcatgga agtttatcac ttccagatgc agattgtgtt gccttggccg 14400gcgatacatt ctggcccctg cccatcacgt agaaagtgct

gcaggtctcc attcaatctc 14460agcgtctggt aaccgagcat acgctgtgag aaagcccgga ctaacatcag tgaacggcac 14520tctagtacca ggacttcgga gcctcgtgct gggcggcaaa cgagctgtta aacgaggagt 14580ggttaacctc gtcaagtatg gccggtaaaa accagagcca gaagaaaaag aaaagtacag 14640ctccgatggg gaatggccag ccagtcaatc aactgtgcca gttgctgggt gcaatgataa 14700agtcccagcg ccagcaacct aggggaggac aggccaaaaa gaaaaagcct gagaagccac 14760attttcccct ggctgctgaa gatgacatcc ggcaccacct cacccagact gaacgctccc 14820tctgcttgca atcgatccag acggctttca atcaaggcgc aggaactgcg tcgctttcat 14880ccagcgggaa ggtcagtttt caggttgagt ttatgctgcc ggttgctcat acagtgcgcc 14940tgattcgcgt gacttctaca tccgccagtc agggtgcaag ttaatttgac agtcaggtga 15000atggccgcga ttggcgtgtg gcctctgagt cacctattca attagggcga tcacatgggg 15060gtcatactta atcaggcagg aaccatgtga ccgaaattaa aaaaaaaaaa a 151111515182DNAPorcine reproductive and respiratory syndrome virus 15tttctccacc cctttaacca tgtctgggat acttgatcgg tgcacgtgta cccccaatgc 60cagggtgttt atggcggagg gccaagtcta ctgcacacga tgcctcagtg cacggtctct 120ccttcccctg aacctccaag tttctgagct cggggtgcta ggcctattct acaggcccga 180agagccactc cggtggacgt tgccacgtgc attccccact gttgagtgct cccccgccgg 240ggcctgctgg ctctctgcaa tctttccaat cgcacgaatg accagtggaa acctgaactt 300ccaacaaaga atgatacggg tcgcagctga gctttacaga gccggccagc tcacccctgc 360agtcttgaag gctctacaag tttatgaacg gggttgccgc tggtacccca ttgttggacc 420tgcccctgga gtggccgttt acgccaattc cctacatgtg agtgataaac ctttcccggg 480agcaactcac gtgttgacca acctgccgct cccgcagaga cccaagcctg aagacttttg 540cccctttgag tgtgctatgg ctactgtcta tgacattggt catgacgccg tcatgtatgt 600ggccgaaagg aaaatctcct gggcccctcg tggcggggat gaagtgaaat ttgaagctgt 660ccccggggag ttgaggttga ttgcgaaccg gctccgcacc tccttcccgc cccaccacac 720agtggacatg tctaagttcg ccttcacagc ccctgggtgt ggtgtttcta tgcgggttga 780acgccaacac ggctgccttc ccgctgacac tgtccctgaa ggcaactgct ggtggagctt 840gtttgactcg cttccactgg aagttcagaa caaagaaatt cgccatgcta accaatttgg 900ctaccagacc aagcatggtg tctctggcaa gtacctacag cggaggctgc aagttaatgg 960tctccgagca gtaactgacc taaacggacc tatcgtcgta cagtacttct ccgttaagga 1020gagttggatc cgccatttga aactggcggg agaacccagc tactctgggt ttgaggacct 1080cctcagaata agggttgagc ctaacacgtc gccattggct gacaaggaag aaaaaatttt 1140ccggtttggc agtcacaagt ggtacggcgc tggaaagaga gcaagaaaag cacgctcttg 1200tgcgactgcc acagtcgctg gccgcgcttt gtccgttcgt gaaacccggc aggccaagga 1260gcacgaggtt gccggcgcca acaaggctga gcacctcaaa cactactccc cgcctgccga 1320agggaattgt ggttggcatt gcatttccgc catcgccaac cggatggtga attccaaatt 1380tgaaaccacc cttcccgaaa gagtgagacc tccagatgac tgggctactg acgaggatct 1440tgtgaatgcc atccaaatcc tcagactccc tgcggcctta gacaggaacg gtgcttgtac 1500tagcgccaag tacgtactta agctggaagg tgagcattgg actgtcactg tggcccctgg 1560gatgtcccct tctttgctcc ctcttgaatg tgttcagggc tgttgtgggc acaagggcgg 1620tcttggttcc ccagatgcag tcgaggtctc cggatttgac cctgcctgcc ttgaccggct 1680ggctgaggtg atgcacctgc ctagcagtgc tatcccagcc gctctggccg aaatgtctgg 1740cgattccgat cgttcggctt ctccggtcac caccgtgtgg actgtttcgc agttctttgc 1800ccgtcacagc ggagggaatc accctgatca agtgcgctta gggaaaatta tcagcctttg 1860tcaggtgatt gaggactgct gctgttccca gaacaaaacc aaccgggtca ccccggagga 1920ggtcgcagca aagattgacc tgtacctccg tggtgcaaca aatcttgaag aatgcttggc 1980caggcttgag aaagcgcgcc cgccacgcgt aatcgacacc ttctttgatt gggatgttgt 2040gctccctggg gttgaggcgg caacccagac gatcaagctg ccccaggtca accagtgtcg 2100tgctctggtc cctgttgtga ctcaaaagtc cttggacgac aactcggtcc ccctgaccgc 2160cttttcactg gctaactact actaccgtgc gcaaggtgac gaagttcgtc accgtgaaag 2220actaaccgcc gtgctctcca agttggaaaa ggttgttcga gaagaatatg ggctcatgcc 2280aaccgagcct ggtccacggc ccacactgcc acgcgggctc gacgaactca aagaccagat 2340ggaggaggac ttgctgaagc tggctaacgc ccagacgact tcggacatga tggcctgggc 2400agtcgagcag gttgacctaa aaacttgggt caagaactac ccgcggtgga caccaccacc 2460ccctccgcca aaagttcagc ctcgaaaaac gaagcctgtc aagagcttgc cggagagaaa 2520gcctgtcccc gccccgcgca ggaaggttgg gtccgattgt ggcagcccgg tttcattagg 2580cggcgatgtc tctaacagtt gggaagattt ggctgttagt agcccctttg atctcccgac 2640cccacctgag ccggcaacac cttcaagtga gctggtgatt gtgtcctcac cgcaatgcat 2700cttcaggccg gcgacaccct tgagtgagcc ggctccaatt cccgcacctc gcggaactgt 2760gtctcgaccg gtgacaccct tgagtgagcc gatccctgtg cccgcaccgc ggcgtaagtt 2820tcagcaggtg aaaagattga gttcggcggc ggcaatccca ccgtaccaga acgagcccct 2880ggatttgtct gcttcctcac agactgaaca tgaggcctct cccccagcac cgccgcagag 2940cgggggcgtt ccgggagtag aggggcatga agctgaggaa accctgagtg aaatctcgga 3000catgtcgggt aacattaaac ctgcgtccgt gtcatcaagc agctccttgt ccagcgtgag 3060aatcacacgc ccaaaatact cagctcaagc catcatcgac tcgggcgggc cctgcagtgg 3120gcatctccaa gaggtaaagg aaacatgcct tagtgtcatg cgcgaggcat gtgatgcgac 3180taagcttgat gaccctgcta cgcaggaatg gctttctcgc atgtgggatc gggtggacat 3240gctgacttgg cgcaacacgt ctgtttacca ggcgatttgc accttagatg gcaggttaaa 3300gttcctccca aaaatgatac tcgagacacc gccgccctat ccgtgtgagt ttgtgatgat 3360gcctcacacg cctgcacctt ccgtaggtgc ggagagcgac cttaccattg gctcagttgc 3420tactgaagat gttccacgca tcctcgagaa aatagaaaat gtcggcgaga tggccaacca 3480gggacccttg gccttctccg aggataaacc ggtagatgac caacttgtca acgacccccg 3540gatatcgtcg cggaggcctg acgagagcac atcagctccg tccgcaggca caggtggcgc 3600cggctctttt accgatttgc cgccttcaga tggcgcggat acggacgggg gggggccgtt 3660tcggacggca aaaagaaaag ctgaaaggct ctttgaccaa ctgagccgtc aggtttttga 3720cctcgtctcc catctccctg ttttcttctc acgccttttc taccctggcg gtggttattc 3780tccgggtgat tggggttttg cagcttttac tctattgtgc ctctttttat gttacagtta 3840cccagccttt ggtattgctc ccctcttggg tgtgttttct gggtcttctc ggcgcgttcg 3900aatgggggtt tttggctgct ggttggcttt tgctgttggt ctgttcaagc ctgtgtccga 3960cccagtcggc gctgcttgtg agtttgactc gccagagtgt agaaacatcc ttcattcttt 4020tgagcttctc aaaccttggg accctgttcg cagccttgtt gtgggccccg tcggtctcgg 4080tcttgccatt cttggcaggt tactgggcgg ggcacgctgc atctggcact ttttgcttag 4140gcttggcatt gttgcagact gtatcttggc tggagcttac gtgctttctc aaggtaggtg 4200taaaaagtgc tggggatctt gtataagaac tgctcctaat gaggtcgctt ttaacgtgtt 4260tcctttcaca cgtgcgacca ggtcgtcact tatcgacctg tgcgatcggt tttgtgcgcc 4320aaaaggaatg gaccccattt ttctcgccac tgggtggcgc gggtgctggg ccggccgaag 4380ccccattgag caaccctctg aaaaacccat cgcgtttgcc caattggatg aaaagaagat 4440tacggctagg actgtggtcg cccagcctta tgaccccaac caagccgtaa agtgcttgcg 4500ggtattgcag gcgggtgggg cgatggtggc taaggcggtc ccaaaagtgg ttaaggtttc 4560cgctgttcca ttccgagctc ccttctttcc cactggagtg aaagttgacc ctgattgcag 4620ggtcgtggtt gaccctgaca ctttcactgc agctctccgg tctggctact ccaccacaaa 4680cctcgtcctt ggtgtagggg actttgccca gctaaatgga ttaaaaatca ggcaaatttc 4740caagccttca gggggaggcc cacatctcat ggctgccctg catgttgcct gctcgatggc 4800tctgcacatg cttgctggga tttatgtgac tgcggtgggt tcttgcggca ccggcaccaa 4860cgacccgtgg tgcgctaacc cgtttgccgt ccctggctac ggacctggct ctctctgtac 4920gtccagattg tgcatttccc aacacggcct taccctgccc ttgtcagcac ttgtggcggg 4980attcggtatt caagaaattg ccttggtcgt tttgattttt gtttccatcg gaggcatggc 5040tcataggttg agctgtaagg ctgacatgct gtgtgttttg cttgcaattg ccagctatgt 5100ttgggtacct cttacctggt tgctttgtgt gtttccttgc tggttgcgct gtttttcttt 5160gcaccccctc accatcctat ggttggtgtt tttcttgatt tctgtgaata tgccttcagg 5220aatcttggcc atggtgttgt tggtttctct ttggcttctt ggtcgttata ctaatgttgc 5280tggccttgtc accccctacg acattcatca ttacaccagt ggcccccgcg gtgttgccgc 5340cttggctacc gcaccagatg ggacctactt ggccgctgtc cgccgcgctg cgttgactgg 5400ccgcaccatg ctgtttaccc cgtcccagct tgggtctctt cttgagggtg ccttcagaac 5460tcgaaagccc tcactgaaca ccgtcaatgt gatcgggtcc tccatgggct ctggcggggt 5520gtttaccatc gacgggaaag tcaagtgcgt aactgccgca catgtcctta cgggcaattc 5580agctcgggtt tccggggtcg gcttcaatca aatgcttgac tttgacgtaa agggagattt 5640cgctatcgct gattgcccga attggcaagg ggctgccccc aagacccaat tctgcacgga 5700tggatggact ggccgtgcct attggctaac atcctctggc gtcgaacccg gcgtcattgg 5760aaaaggattc gccttctgct tcaccgcatg tggcgattcc gggtccccag tgatcaccga 5820ggccggtgag cttgtcggcg ttcacacggg atcgaataaa caaggggggg gcattgttac 5880gcgcccctca ggccagtttt gtaatgtggc acccatcaag ctaagcgaat taagtgaatt 5940ctttgctggg cctaaggtcc cgctcggtga tgtgaaggtc ggcagccaca taattaaaga 6000cataagcgag gtgccttcag atctttgtgc cttgcttgct gccaaacctg aactggaagg 6060aggcctctcc accgtccaac ttctttgtgt gttttttctc ctgtggagaa tgatgggaca 6120tgcctggacg cccttggttg ctgtgagttt ctttattttg aatgaggttc tccctgccgt 6180cctggtccgg agtgttttct cctttggaat gtttgtgcta tcctggctca cgccatggtc 6240tgcgcaagtt ctgatgatca ggcttctgac agcagctctt aacaggaaca gatggtcact 6300tgcctttttc agcctcggtg cagtgaccgg ttttgtcgca gatcttgcgg ccactcaggg 6360gcatccgttg caggcagtga tgaatttgag cacctatgca ttcctgcctc ggatgatggt 6420tgtgacctca ccagtcccag tgatcacgtg tggtgtcgtg cacctacttg ccatcatttt 6480gtacttgttt aagtaccgtg gcctgcacca tatccttgtt ggcgatggag tgttctctgc 6540ggctttcttc ttgagatact ttgccgaggg aaagttgagg gaaggggtgt cgcaatcctg 6600cggaatgaat catgagtctc tgactggtgc cctcgctatg agactcaatg acgaggactt 6660ggatttcctt atgaaatgga ctgattttaa gtgctttgtt tctgcgtcca acatgaggaa 6720tgcagcgggt caatttatcg aggctgccta tgctaaagca cttagagtag aactggccca 6780gttggtacag gttgataaag ttcgaggtac tttggccaaa cttgaagctt ttgctgatac 6840cgtggctcct caactctcgc ccggtgacat tgttgtcgct ctcggccaca cgcctgttgg 6900cagtatcttc gacctaaagg ttggtagcac caagcatacc ctccaagcca ttgagaccag 6960agtccttgct gggtccaaaa tgaccgtggc gcgcgtcgtc gacccgaccc ccacgccccc 7020acccgcaccc gtgcccatcc ccctcccacc gaaagttctg gagaatggcc ccaacgcttg 7080gggggatgag gaccgtttga ataagaagaa gaggcgcagg atggaagccc tcggcatcta 7140tgttatgggc gggaaaaagt accagaaatt ttgggacaag aattccggtg atgtgtttta 7200tgaggaggtc cataataaca cagatgagtg ggagtgtctc agagttggcg accctgccga 7260ctttgaccct gagaagggaa ctctgtgtgg acatgtcacc attgaaaata aggcttacca 7320tgtttacacc tccccatctg gtaagaagtt cttggtcccc gtcaacccag agaatggaag 7380agttcaatgg gaagctgcaa agctttccgt ggagcaggcc ctaggtatga tgaatgtcga 7440cggcgaactg actgccaaag aactggagaa actgaaaaga ataattgaca aactccaggg 7500cctgactaag gagcagtgtt taaactgcta gccgccagcg acttgacccg ctgtggtcgc 7560ggcggcttgg ttgttactga aacagcggta aaaatagtca aatttcacaa ccggaccttc 7620accctgggac ctgtgaattt aaaagtggcc agtgaggttg agctaaaaga cgcggttgag 7680cacaaccaac acccggttgc gagaccgatc gatggtggag ttgtgctctt gcgttccgcg 7740gttccttcgc ttatagacgt cttgatctcc ggtgctgatg catctcccaa gttacttgcc 7800catcacgggc cgggaaacac tgggatcgat ggcacgctct gggattttga gtccgaagcc 7860actaaagagg aagtcgcact cagtgcgcaa ataatacagg cttgtgacat taggcgcggc 7920gacgctcctg aaattggtct cccttacaag ctgtaccctg ttaggggtaa ccctgagcgg 7980gtgaaaggag ttctgcagaa tacaaggttt ggagacatac cttacaaaac ccccagtgac 8040actggaagcc cagtgcacgc ggctgcctgc cttacgccca acgccactcc ggtgactgat 8100gggcgctccg tcttggccac gaccatgccc cccgggtttg agttatatgt accgaccata 8160ccagcgtctg tccttgatta ccttgactct aggcctgact gccctaaaca gctgacagag 8220cacggctgcg aagatgccgc actgaaagac ctctctaaat atgacttgtc cacccaaggc 8280tttgttttac ctggagttct tcgccttgtg cggaaatacc tgtttgccca tgtaggtaag 8340tgcccacccg ttcatcggcc ttctacttac cctgctaaga attctatggc tggaataaat 8400gggaacaggt tcccaaccaa ggacattcag agcgtccctg aaatcgacgt tctgtgcgca 8460caggctgtgc gagaaaactg gcaaactgtc accccttgta ctcttaagaa acagtattgc 8520gggaagaaga agactaggac catactcggc accaataact tcatcgcact agcccaccga 8580gcagtgttga gtggtgttac ccagggcttc atgaaaaagg cgtttaactc gcccatcgcc 8640ctcggaaaga acaagtttaa ggagctacag actccggtcc tgggcaggtg ccttgaagct 8700gatctcgcat cctgcgatcg atccacgcct gcaattgtcc gctggtttgc cgccaacctt 8760ctttatgaac ttgcctgtgc tgaagagcat ctaccgtcgt acgtgctgaa ctgctgccac 8820gacttactgg tcacgcagtc cggcgcagtg actaagagag gtggcctgtc gtctggcgac 8880ccgatcacct ctgtgtctaa caccatttat agtttggtga tctatgcaca gcatatggtg 8940cttagttact tcaaaagtgg tcacccccat ggccttctgt tcttacaaga ccagctaaag 9000tttgaggaca tgctcaaggt tcaacccctg atcgtctatt cggacgacct cgtgctgtat 9060gccgagtctc ccaccatgcc aaactatcac tggtgggttg aacatctgaa tttgatgctg 9120gggtttcaga cggacccaaa gaagacagca ataacagact cgccatcatt tctaggctgt 9180agaataataa atgggcgcca gctagtcccc aaccgtgaca ggatcctcgc ggccctcgcc 9240tatcacatga aggcgagtaa tgtttctgaa tactatgcct cagcggctgc aatactcatg 9300gacagctgtg cttgtttgga gtatgatcct gaatggtttg aagaacttgt agttggaata 9360gcgcagtgcg cccgcaagga cggctacagt tttcccggca cgccgttctt catgtccatg 9420tgggaaaaac tcaggtccaa ttatgagggg aagaagtcga gagtgtgcgg gtactgcggg 9480gccccggccc cgtacgctac tgcctgtggc ctcgacgtct gcatttacca cacccacttc 9540caccagcatt gtccagtcac aatctggtgt ggccatccag cgggttctgg ttcttgtagt 9600gagtgcaaat cccctgtagg gaaaggcaca agccctttag acgaggtgct ggaacaagtc 9660ccgtataagc ccccacggac cgttatcatg catgtggagc agggtctcac cccccttgat 9720ccaggtagat accaaactcg ccgcggacta gtctctgtca ggcgtggaat taggggaaat 9780gaagttgaac taccagacgg tgattatgct agcaccgcct tgctccctac ctgcaaagag 9840atcaacatgg tcgctgtcgc ttccaatgta ctgcgcagca ggttcatcat cggcccaccc 9900ggtgctggga aaacatactg gctccttcaa caggtccagg atggtgatgt tatttacaca 9960ccaactcacc agaccatgct tgacatgatt agggctttgg ggacgtgccg gttcaacgtc 10020ccggcaggca caacgctgca attccccgtc ccctcccgca ccggtccgtg ggttcgcatc 10080ctagccggcg gttggtgtcc tggcaagaat tccttcctag atgaagcagc gtattgcaat 10140caccttgatg ttttgaggct tcttagtaaa actaccctca cctgtctagg agacttcaag 10200caactccacc cagtgggttt tgattctcat tgctatgttt ttgacatcat gcctcaaact 10260caactgaaga ccatctggag gtttggacag aatatctgtg atgccgttca gccagattac 10320agggacaaac tcatgtccat ggtcaacaca acccgtgtga cctacgtgga aaaacctgtc 10380aggtatgggc aggtcctcac cccctaccac agggaccgag aggacgacgc catcactatt 10440gactccagtc aaggcgccac attcgatgtg gttacattgc atttgcccac taaagattca 10500ctcaacaggc aaagagccct tgttgccatc accagggcaa gacacgctat ctttgtgtat 10560gacccacaca ggcagctgca gggcttgttt gatcttcctg caaaaggcac acccgtcaac 10620ctcgcagtgc accgcgacgg gcagctgatc gtgctggata gaaataacaa agaatgcacg 10680gtcgctcagg ctctaggcaa cggggataaa tttagggcca cagataagcg tgttgtagat 10740tctctccgcg ccatttgtgc tgatctagaa gggtcgagct ctccgctccc caaggtcgca 10800cacaacttgg gattttattt ctcacctgat ttaacacagt ttgctaaact cccagtagaa 10860cttgcacctc actggcccgt ggtgacaacc cagaacaatg aaaagtggcc agatcggctg 10920gttgccagcc ttcgccctat ccataaatac agccgcgcgt gcatcggtgc cggctatatg 10980gtgggccctt cggtgtttct aggcactcct ggggtcgtgt catactatct cacaaaattt 11040gttaagggcg aggctcaatt gcttccggag acggttttca gcaccggccg aattgaggta 11100gactgccggg aatatcttga tgatcgggag cgagaagttg ctgcgtccct cccacacgct 11160ttcattggcg acgtcaaagg cactaccgtt ggaggatgtc atcatgtcac ctccagatac 11220ctcccacgcg tccttcccaa ggaatcagtt gcggtagtcg gggtttcaag ccccggaaaa 11280gccgcgaaag cattgtgcac actgacagat gtgtacctcc cagatcttga cgcctatctc 11340cacccggaga cccagtccaa gtgctggaaa atgatgttgg acttcaaaga agttcgacta 11400atggtctgga aagacaaaac agcctatttc caacttgaag gtcgctattt cacctggtat 11460cagcttgcca gctatgcctc gtacatccgt gttcctgtca actctacggt gtacttggac 11520ccctgcatgg gccccgccct ttgcaacagg agagtcgtcg ggtccaccca ctggggggct 11580gacctcgcgg tcacccctta tgattacggc gctaaaatta tcctgtctag cgcgtaccat 11640ggtgaaatgc cccccggata caaaattctg gcgtgcgcgg agttctcgtt ggatgaccca 11700gttaagtaca aacatacctg ggggtttgaa tcggatacag cgtatctgta tgagttcacc 11760ggaaacggtg aggactggga ggattacaat gatgcgtttc gtgcgcgcca ggaagggaaa 11820atttataagg ccactgccac cagcttgaag ttttattttc ccccgggccc tgtcattgaa 11880ccaactttag gcctgaattg aaatgaaatg gggtccatgc aaagcctttt ttacaaaatt 11940ggccaacttt ttgtggatgc tttcacggag ttcttggtgt ccattgttga tatcactata 12000tttttggcca ttttgtttgg cttcaccatc gccggttggc tggtggtctt ttgcatcaga 12060ttggtttgct ccgcgatact ccgtacgcgc tctgccattc actctgagca attacagaag 12120atcttatgag gcctttcttt cccagtgcca agtggacatt cccacctggg gaactaaaca 12180tcctttgggg atgctttggc accataaggt gtcaaccctg attgatgaaa tggtgtcgcg 12240tcgaatgtac cgcatcatgg aaaaagcagg gcaggctgcc tggaaacagg tggtgagcga 12300ggctacgctg tctcgcatta gtagtttgga tgtggtggct cattttcagc atctagccgc 12360cattgaagcc gagacctgta aatatttggc ctcccggctg cccatgctac acaacctgcg 12420catgacaggt tcaaatgtaa ccatagtgta taatagcact ttgaatcagg tgtttgctat 12480ttttccaacc cctggttccc ggccaaagct taatgatttt cagcaatggt taatagctgt 12540acattcctcc atattttcct ctgttgcaac ttcttgtact ctttttgttg tgctgtggtt 12600gcgggttcca atactacgta ctgcttttgg tttccgctgg ttaggggcaa tttttctttc 12660gaactcacag tgaattacac ggtgtgtcca ccttgcctca cccggcaagc agccgcagag 12720atctacgaac ccggtaggtc tctttggtgc aggatagggt atgaccgatg tgaggaggat 12780gatcatgacg agctagggtt tatggtaccg cctggcctct ccagcgaagg ccacttgact 12840agtgtttacg cctggttggc gttcttgtcc ttcagctaca cggcccagtt ccatcccgag 12900atattcggga tagggaatgt gagtcgagtt tatgttgaca tcaaacatca actcatctgc 12960gccgaacatg acgggcagaa caccaccttg cctcgtcatg acaacatttc agccgtgttt 13020cagacctatt accaacatca agtcgacggc ggcaattggt ttcacctaga atggcttcgt 13080cccttctttt cctcgtggtt ggttttaaat gtctcttggt ttctcaggcg ttcgcctgca 13140aaccatgttt cagttcgagt cttgcagata ttaagaccaa caccaccgca gcggcaagct 13200ttgctgtcct ccaagacatc agttgcctta ggcatcgcga ctcggcctct gaggcgattc 13260gcaaaatccc tcagtgccgt acggcgatag ggacacccgt gtatgttacc atcacagcca 13320atgtgacaga tgagaattat ttacattctt ctgatctcct catgctttct tcttgccttt 13380tctatgcttc tgagatgagt gaaaagggat ttaaggtggt atttggcaat gtgtcaggca 13440tcgtggctgt gtgtgtcaat tttaccagct acgtccaaca tgtcaaggag tttacccaac 13500gctccctggt ggtcgaccat gtgcggttgc tccatttcat gacacctgag accatgaggt 13560gggcaactgt tttagcctgt ctttttgcca ttctgttggc aatttgaatg tttaagtatg 13620ttggagaaat gcttgaccgc gggctgttac tcgcaattgc tttctttgtg gtgtatcgtg 13680ccgttctgtt ttgctgtgct cgtcaacgcc agcaacgaca gcagctccca tctacagctg 13740atttacaact tgacgctatg tgagctgaat ggcacagatt ggctagctaa caaatttgat 13800tgggcagtgg agagttttgt catctttccc gttttgactc acattgtctc ctatggtgcc 13860ctcactacta gccatttcct tgacacagtc gctttagtca ctgtgtctac cgccgggttt 13920gttcacgggc ggtatgtcct aagtagcatc tacgcggtct gtgccctggc tgcgttgact 13980tgcttcgtca ttaggtttgc aaagaattgc atgtcctggc gctacgcgtg taccagatat 14040accaactttc ttctggacac taagggcaga ctctatcgtt ggcggtcgcc tgtcatcata 14100gagaaaaggg gcaaagttga ggtcgaaggt catctgatcg acctcaaaag agttgtgctt 14160gatggttccg tggcaacccc tataaccaga gtttcagcgg aacaatgggg tcgtccttag 14220atgacttctg tcatgatagc acggctccac aaaaggtgct tttggcgttt tctattacct 14280acacgccagt gatgatatat gccctaaagg tgagtcgcgg

ccgactgcta gggcttctgc 14340accttttgat cttcctgaat tgtgctttca ccttcgggta catgactttc gcgcactttc 14400agagtacaaa taaggtcgcg ctcactatgg gagcagtagt tgcactcctt tggggggtgt 14460actcagccat agaaacctgg aaattcatca cctccagatg ccgtttgtgc ttgctaggcc 14520gcaagtacat tctggcccct gcccaccacg ttgaaagtgc cgcaggcttt catccgattg 14580cggcaaatga taaccacgca tttgtcgtcc ggcgtcccgg ctccactacg gtcaacggca 14640cattggtgcc cgggttaaaa agcctcgtgt tgggtggcag aaaagctgtt aaacagggag 14700tggtaaacct tgtcaaatat gccaaataac aacggcaagc agcagaatag aaagaagggg 14760gatggccagc cagtcaatca gctgtgccag atgctgggta agatcatcgc tcagcaaaac 14820cagtccagag gcaagggacc gggaaagaaa aataagaaga aaaacccgga gaagccccat 14880tttcctctag cgactgaaga tgatgtcaga catcacttta cccctagtga gcggcaattg 14940tgtctgtcgt caatccagac cgcctttaat caaggcgctg ggacttgcac cctgtcagat 15000tcagggagga taagttacac tgtggagttt agtttgccta cgcatcatac tgtgcgcctg 15060attcgcgtca cagcatcacc ctcagcatga tgggctggca ttcttgaggc atctcagtgt 15120ttgaattgga agaatgtgtg gtgaatggca ctgattgaca ttgtgcctct aagcactata 15180tt 1518216383PRTPorcine reproductive and respiratory syndrome virus 16Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp 195 200 205Asp Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val 210 215 220Glu Ile Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala225 230 235 240Asp Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr 245 250 255Tyr His Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser 260 265 270Lys Cys Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu 275 280 285Tyr Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly 290 295 300Val Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg305 310 315 320Ala Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys 325 330 335Pro Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro 340 345 350Gly Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro 355 360 365Thr Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 370 375 38017382PRTPorcine reproductive and respiratory syndrome virus 17Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Pro Asn Gly Met Met Trp Thr Pro Glu Ser Asp Asp 195 200 205Ser Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu 210 215 220Ile Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp225 230 235 240Trp Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr 245 250 255His Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys 260 265 270Cys Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr 275 280 285Glu Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val 290 295 300Ser Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala305 310 315 320Val Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro 325 330 335Gln Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly 340 345 350Phe Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr 355 360 365Thr Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 370 375 38018381PRTPorcine reproductive and respiratory syndrome virus 18Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser 195 200 205Ala Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile 210 215 220Leu Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp225 230 235 240Glu Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His 245 250 255Ser Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys 260 265 270Trp Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu 275 280 285Asp Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser 290 295 300Gly Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val305 310 315 320Ile Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln 325 330 335Ser Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe 340 345 350Val Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr 355 360 365Leu Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 370 375 38019380PRTPorcine reproductive and respiratory syndrome virus 19Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala 195 200 205Asp Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu 210 215 220Ile Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu225 230 235 240Leu Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser 245 250 255Gly Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp 260 265 270Leu Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp 275 280 285Tyr Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly 290 295 300Lys Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile305 310 315 320Asp Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser 325 330 335Trp Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val 340 345 350Arg Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu 355 360 365Arg Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 370 375 38020379PRTPorcine reproductive and respiratory syndrome virus 20Met Ser Gly Thr Phe Ser Arg Cys Met Cys Thr Pro Ala Ala Arg Val1 5 10 15Phe Trp Asn Ala Gly Gln Val Tyr Cys Thr Arg Cys Leu Ser Ala Arg 20 25 30Ser Leu Leu Pro Pro Glu Leu Gln Asp Ile Asp Leu Ala Ala Ile Gly 35 40 45Leu Phe Tyr Lys Pro Lys Asp Lys Leu His Trp Lys Val Pro Ile Gly 50 55 60Ile Pro Gln Val Glu Cys Thr Pro Ser Gly Cys Cys Trp Leu Ser Gly65 70 75 80Ile Phe Pro Leu Ala Arg Met Thr Ser Gly Asn His Asn Phe Leu Gln 85 90 95Arg Leu Val Lys Val Ala Asp Val Leu Tyr Arg Asp Gly Cys Leu Thr 100 105 110Ser Arg His Leu Arg Glu Leu Gln Val Tyr Glu Arg Gly Cys Ser Trp 115 120 125Tyr Pro Ile Thr Gly Pro Val Pro Gly Met Gly Leu Tyr Ala Asn Ser 130 135 140Met His Val Ser Asp Gln Pro Phe Pro Gly Ala Thr His Val Leu Thr145 150 155 160Asn Ser Pro Leu Pro Gln Gln Ala Cys Arg Gln Pro Phe Cys Pro Phe 165 170 175Glu Glu Ala His Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe 180 185 190Thr Asp Ser Ser Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp 195 200 205Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile 210 215 220Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu225 230 235 240Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly 245 250 255Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu 260 265 270Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr 275 280 285Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys 290 295 300Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp305 310 315 320Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp 325 330 335Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg 340 345 350Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg 355 360 365Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 370 37521203PRTPorcine reproductive and respiratory syndrome virus 21Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp 20 25 30Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile 35 40 45Arg Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu 50 55 60Ala Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly65 70 75 80Gly Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu 85 90 95Ser Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr 100 105 110Leu Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys 115 120 125Tyr Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp 130 135 140Pro Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp145 150 155 160Ile Arg His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg 165 170 175Leu Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg 180 185 190Ile Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195 20022202PRTPorcine reproductive and respiratory syndrome virus 22Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Gly Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu 20 25 30Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg 35 40 45Ser Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala 50 55 60Glu Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly Gly65 70 75 80Tyr Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu Ser 85 90 95Cys Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu 100 105 110Ala Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr 115 120 125Leu Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro 130 135 140Asp Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile145 150 155 160Arg His Leu Thr

Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu 165 170 175Thr Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile 180 185 190Phe Arg Phe Gly Ala His Lys Trp Tyr Gly 195 20023201PRTPorcine reproductive and respiratory syndrome virus 23Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu 20 25 30Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 45Phe Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala Glu 50 55 60Ser Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly Gly Tyr65 70 75 80Leu Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu Ser Cys 85 90 95Phe Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu Ala 100 105 110Asn Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu 115 120 125Gln Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro Asp 130 135 140Gly Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg145 150 155 160His Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr 165 170 175Ser Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile Phe 180 185 190Arg Phe Gly Ala His Lys Trp Tyr Gly 195 20024200PRTPorcine reproductive and respiratory syndrome virus 24Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala 20 25 30Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser Phe 35 40 45Pro Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala Glu Ser 50 55 60Pro Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly Gly Tyr Leu65 70 75 80Val Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu Ser Cys Phe 85 90 95Leu Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu Ala Asn 100 105 110Ala Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu Gln 115 120 125Arg Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro Asp Gly 130 135 140Pro Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg His145 150 155 160Leu Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr Ser 165 170 175Leu Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile Phe Arg 180 185 190Phe Gly Ala His Lys Trp Tyr Gly 195 20025199PRTPorcine reproductive and respiratory syndrome virus 25Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu 20 25 30Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser Phe Pro 35 40 45Ala His His Pro Val Ser Leu Ala Asp Trp Glu Leu Ala Glu Ser Pro 50 55 60Glu Asn Gly Phe Ser Phe Ser Thr Tyr His Ser Gly Gly Tyr Leu Val65 70 75 80Gln Asn Pro Asp Val Phe Asp Ser Lys Cys Trp Leu Ser Cys Phe Leu 85 90 95Asp Gln Pro Ile Glu Val Arg Leu Tyr Glu Asp Tyr Leu Ala Asn Ala 100 105 110Phe Gly Tyr Gln Thr Lys Trp Gly Val Ser Gly Lys Tyr Leu Gln Arg 115 120 125Arg Leu Gln Val Asn Gly Ile Arg Ala Val Ile Asp Pro Asp Gly Pro 130 135 140Ile His Val Glu Ala Leu Ser Cys Pro Gln Ser Trp Ile Arg His Leu145 150 155 160Thr Leu Asp Asp Asp Val Thr Pro Gly Phe Val Arg Leu Thr Ser Leu 165 170 175Arg Ile Val Pro Asn Thr Glu Pro Thr Thr Leu Arg Ile Phe Arg Phe 180 185 190Gly Ala His Lys Trp Tyr Gly 1952650PRTPorcine reproductive and respiratory syndrome virus 26Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp 20 25 30Leu Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile 35 40 45Arg Ser 502749PRTPorcine reproductive and respiratory syndrome virus 27Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Gly Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu 20 25 30Glu Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg 35 40 45Ser2848PRTPorcine reproductive and respiratory syndrome virus 28Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu 20 25 30Ala Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 452947PRTPorcine reproductive and respiratory syndrome virus 29Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Pro Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala 20 25 30Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 453046PRTPorcine reproductive and respiratory syndrome virus 30Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Asp Leu Glu Ala Leu 20 25 30Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 453115110DNAPorcine reproductive and respiratory syndrome virus 31atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacggtcg gatgatgtgg acgccggaat 840ccgatgattc agccgaccta gaggcgctac cgcctgagct agaacgtcag gtcgaaatcc 900tcattcggag ttttcctgcc catcaccctg tcagcctggc cgactgggag ctcgctgagt 960cccctgagaa cggtttttcc ttcagcacgt accattctgg tggttatctt gtccaaaacc 1020ccgacgtgtt tgacagcaag tgctggctct cctgtttctt ggatcagccg atcgaagtgc 1080gcctctatga ggattatctg gctaacgctt tcggttacca aaccaagtgg ggcgtgtctg 1140gtaagtacct ccagcgcagg cttcaagtca acggtattcg tgctgtaatc gatcctgatg 1200gccccattca cgttgaagcg ctgtcttgcc cccaatcttg gatcaggcac ctgactctgg 1260acgatgacgt caccccagga ttcgttcgcc taacatccct ccgcattgtg ccgaacacag 1320agcccactac tctccgaatc tttcggtttg gagcgcataa gtggtatggc gctgccggca 1380aacgggctcg tgccaagcgt gccgctaaaa gtgagaaggg tccggctccc actcccaagg 1440ttgccccgcc agcccccacc tgcggaattg ttacctactc tccaccaaca gacgggtctt 1500gcggttggca cgtccttgcc gccataatga accgaatgat gaacggtgac ttcatgtccc 1560ctctggccca gtacaacaga ccagaggatg attgggcttc tgattacgat cttgcccagg 1620cgattcaatg tctgcggttg cctgctacca tagttcggaa tcgtgcctgt cccaacgcca 1680agtacctcat aaaactcaac ggagtccact gggaggtaga ggtgaggtca gggatggccc 1740ctcgctccct tccccgcgag tgcgtagtcg gcgtttgttc cgaaggctgt gccgcatcgc 1800cttacccaga aaacgggcta cctaaacgag cgtttgaggc cttggcgtct gcttacagac 1860taccttccga ttgtgtcagt tctggtattg ctgactttct tgctaacccc cctcaggaat 1920tctggactct tgacaaaatg ttgacctccc cgtcaccgga gcggtctggt ttttccagtc 1980tgtacaaatt gctattagag gttgtcccgc agaaatgcgg agccacggaa ggggctttca 2040cctatgctgt tgagagaatg ctgaaggatt gcccgagctc cgaacaggcc atggcccttc 2100tggcaaaaat taaagttcca tcctcaaagg ccccgtctgt gtccctggac gagtgtttcc 2160ctacggatgt ttcagccgat ttcgaaccag catctcagga gaggtcccaa aattccagcg 2220ctgctgttgt cctgcattca ccgaatgcaa aagagttcga ggaagcagct ccaggggaag 2280ttcaggaggg tggccacaag gccgtccact ctgcactccc tgccgggggt cctaacaata 2340agcaggcaca gctggttgcc ggtgagcaac tgaagctcgg cggttgtggt tcggtagttg 2400ggaatgcaca tgaaggtgtt ctggtcccac ctggtccaat taatttgaca agcggggatt 2460taccctcctc aggctccatg aaagaagata tgctcaatag ccgggaggac gaaccactgg 2520atttgtccca accagcaaca gctgtcacaa cgactcttat gggagagcta acacccgact 2580acctaggttc tgatactggt gccctccccg tcaccgtccg aaaatttgtc ccgacggggc 2640ctatactccg tcatgttgag cactgcagca cggggtcggg cgatagcagt tcgcctttgg 2700atctgtctgg tgcgcaaacc ccggaccagc ctttaaatct gtccctggcg gcttggccag 2760tgaggaccac cgcgtctgat cctggctggg tccacggtag acgcgagcct gtctttgtaa 2820agcctcgaga tgttttctct gatggcgatt cagcccttca gttcggggag ctttctgaat 2880ccagctctgt catcgagttt gatcgggcaa aagatgctca ggtggctgac gcccctgtcg 2940gtctgacgac ttcggacgag gccctctccg cagtcgatcc tttcgagttt tccgaactca 3000agcgcccacg tttctccgca caagccttaa ttgaccgagg cggcccactt gccgatgtcc 3060atgcaaaaat aaagaaccgg gtatatgaac agtgcctcca agcttgtgag cccggtagtc 3120gcgcaacccc agctaccagg gagtggctcg acaaaatgtg ggagagagtg gacatgaaaa 3180cctggcgctg cacctcacag ttccaagctg gtcacattct tgcgtccctc aaattcctcc 3240ctgacatgat tcaagacaca ccgcctcctg ttcccaggaa gaaccgagcc agtgataaag 3300ctggcctgaa acaactagtg gcacagtggg ataggagatt gagttcaacc ccccccccaa 3360aaccggttgg gccggtactt gaccgggtcg atcctccgcc tacgggtacc cggcaagaag 3420acgtcacccc ctccgatggg ccaccccatg cgccggatgg tcgagtgagt acgggtggga 3480gttggaaagg ccttatgctt tccggcaccc gtctcacggg gtccatcagt catcgcctca 3540tgacatgggt ttttgaagtt gtctcccatc tcccagcttt tatgctcaca cttttctcgc 3600cgcggggctc tatggctcca ggcgattggg tttttgcagg tgttgtttta cttgctctcc 3660tgctctgtcg ttcttaccca atattcgggt gccttccctt attgggtgtc ttttctggta 3720ctgtgcggcg tgttcgtctg ggtgtttttg gctcttggat ggcttttgct gtatttttat 3780tctcgactcc atccaaccca gtcggttctt cttgtaacca cgattcgccg gagtgtcatg 3840ctgagcttct ggctcttgag cagcgccaac tttgggaacc tgtgcgcggc cttgtggtcg 3900gcccatcggg cctcttatgt gtcattcttg gcaagttact cggtgggtca cgttatctct 3960ggcatgttct cctacgtcta tgcctgcttg cagatttggc cctttctctt gtttatgtgg 4020tgtcccaggg gcgttgtcac aagtgttggg gaaagtgtat aaggacagct cctgctgagg 4080tggcttttaa tgtatttcct ttctcgcgcg ccacccgtag ttctcttgta tccttatgtg 4140atcgattcca aacgccaaaa ggagttgatc ccgtgcactt ggcgacaggt tggcgcgggt 4200gctggcgtgg tgagagtcct atccatcaac cacaccaaaa gcccatagct tacgccaatt 4260tggatgaaaa gaagatatct gcccaaacgg ttgttgctgt cccatacgat cccaatcagg 4320ctatcaaatg tctgaaagtt ctgcaggcgg gaggggctat cgtggatcag cctacgcctg 4380aggtcgttcg tgtgtccgag atccccttct cagccccatt tttcccaaaa gttccagtca 4440acccagattg tagggtcgtg gtggattcgg acacttttgt ggctgcggtt cgttgtggtt 4500attcgacatc acaactggtc ctgggccagg gcaactttgc caagttaaat caaacccccc 4560ccaggaactc tatctccacc aaagcgactg gtggggcctc ttatactttt gctgtggctc 4620aagtgtctgt gtggaccctt gttcatttcg tcctcggtct ttggctcacg tcacctcaag 4680tgtgtggtcg aggaaccgct gacccatggt gttcaagtcc attttcatat cctacctatg 4740gccccggggt tgtgtgctcc tctcgacttt gtgtgtctgc tgacggggtc acccttccat 4800tgttctcagc cgtggcacaa ctctctggta gggaggtggg gatttttatt ttagtgctcg 4860tctcctttat tgccttggcc caccgcatgg ctcttaaggc agacatgtta gtagtctttt 4920tggctctttg tgcttatgcc tggcccatga gctcctggtt gatctgcttc tttcctatac 4980tcttgaggtg ggttaccctt caccctctca ctatgctttg ggtgcattca ttcttgatgt 5040tttgtctccc agcagccggc gtcctctcac tggggataac tggcctcctc tgggcaatcg 5100gccgctttac tcaggttgcc ggaatcatta caccttatga tatccaccag tacacctctg 5160ggccacgtgg tgcagccgct gtggccacag ccccagaagg cacttacatg gccgccgtcc 5220ggagagctgc tttaaccggg cggactttaa tcttcacccc gtccgcagtt ggatcccttc 5280tcgaaggtgc tttcaggact cataaaccct gccttaacac cgtgaatgtc gtaggctcct 5340cccttggttc cggaggggtt ttcaccattg acggaaaaaa aattgtcgtc actgctgccc 5400atgtgctgaa cggcgacaca gctagagtca ccggtgattc ctacaaccgc atgcacactt 5460tcaagactaa tggtgactat gcctggtccc atgctgataa ctggcagggc gctgcccctg 5520tggtcaaggt tgcgaaaggg tatcgcggtc gtgcctactg gcaaacatca actggtgtcg 5580agcctggtgt tattgggaat gggttcgcct tctgtttcac caactgcggc gattcggggt 5640cacccgttat ctcagaatct ggtgatctta tcggaatcca caccggttca aacaaacttg 5700gttctggtct tgtgacaacc cctgaagggg agacctgtac catcagagaa accaagcttt 5760ctgacctttc cagacatttc gcaggcccaa gcgttcctct tggggacatc aaattgagtc 5820cggccatcat ccctgatgtg acatccattc cgagtgactt ggcatcgctc ctagcttccg 5880tccctgtaat ggaaggcggc ctctcgaccg ttcaactttt gtgtgtcttt ttcctcctct 5940ggcgcatgat gggccatgcc tggacgccca ttgttgccgt gggcttcttt ttgctgaatg 6000aaattcttcc agcagttttg gttcgagccg tgttttcttt tgcactcttt gtgcttgcat 6060gggccacccc ctggtctgca caagtgttga tgattagact tctcacggca tctctcaacc 6120gcaacaaact ttctcttgcg ttctacgcac tcggaggtgt tgttggtttg gctgctgaaa 6180tcgggacttt tgctggtaaa ttgtctgaat tgtctcaagc tctttcgaca tactgtttct 6240tacctagggt ccttgctatg accagctgtg ttcccatcat catcattggt ggactccatg 6300ccctcggtgt gattctgtgg ttattcaaat accggtgcct ccacaacatg ctggttggtg 6360atggaagctt ttcaagcgct ttcttcctac ggtattttgc agagggcaat ctcaggagag 6420gtgtttcaca gtcctgtggc atgagtaacg agtccctgac ggctgctttg gcttgcaagt 6480tgtcacaggc tgaccttgat tttttgtcca gcttaacgaa cttcaagtgc tttgtatctg 6540cttcaaacat gaaaaatgct gccggccagt acattgaagc agcttatgcc agggccctgc 6600gtcaagagtt ggcctcttta gtccagattg acaaaatgaa aggagttttg tccaagctag 6660aggcctttgc tgaaacggcc actccgtccc tcgacgtagg tgacgtgatt gttctacttg 6720gacaacatcc tcacggatcc gttctcgata ttaatgtggg gactgaaagg aaaactgtat 6780ccgtgcaaga gacccggagc ctaggcggct ccaagttcag tgtttgtact gttgtgtcaa 6840acacacccgt ggacgcctta gccggtattc cactccagac accaaccccc cttttcgaga 6900atggcccgcg tcatcgcagc gaggaggacg atcttaaagt cgagaggatg aagaaacact 6960gcgtgtccct cggcttccac aacattaacg gtaaagttta ctgcaagatt tgggacaagt 7020ctaccggtga cgccttttac actgatgatt cccggtacac ccaagactat gcttttcagg 7080acaggtcagc tgactataga gacagggact acgagggtgt gcaaaccgcc ccccaacagg 7140gatttgatcc aaagtctgaa acccctgttg gtaccgttgt gatcggcggt attacgtaca 7200acaggtattt ggtcaaaggt aaggaggttc tggttcccaa gcctgacaac tgccttgaag 7260ctgccaagct gtcccttgag caagctctcg ctgggatggg ccaaacttgt gaccttacag 7320ctgccgaggt ggaaaagcta aagcgcatca ttggtcaact tcaaggattg accactgagc 7380aggctttaaa ctgttagccg ccagcggctt gacccgctgt ggccgcggcg gcctagttgt 7440aactgaaacg gcggtaaaaa ttgtcaaata ccacagcaga acttttacct taggctcttt 7500agacctaaaa gtcacttccg aggtggaggt gaagaagtca accgagcagg gccacgctgt 7560tgtggcaaac ttgtgttctg gtgtcgtctt gatgagacct cacccaccgt cccttgtcga 7620cgttcttctg aaacccggac ttgacataac acccggcatt caaccagggc atggggccgg 7680gaatatgggc gtggacggtt ccatttggga ttttgaaacc gcacccacaa aggctgaact 7740cgagttatcc aagcaaataa ttcaagcatg tgaagtcagg cgcggggatg ccccgaacct 7800ccagctccct tacaagctct atcctgttag aggggatcct gagcggcata aaggccacct 7860catcaatacc aggtttggag acttacctta caaaactcct caagacacca agtccgcaat 7920ccacgcggct tgttgcctgc accccaacgg ggcccccgtg tctgatggta aatccacact 7980aggtaccact cttcaacatg gcttcgagct ttatgtccct actgtgccct atagtgtcat 8040ggagtacctt gattcacgcc cagacacccc ttttatgtgc actaaacacg gcacttccaa 8100ggctgctgca gaggacctcc aaaaatacga cctatccacc caaggatttg tcctgcctgg 8160ggtcctacgc ctagtgcgca ggttcatctt tggccacatt ggcaaggcac cgccattgtt 8220cctcccatca acttatcccg ccaagaactc catggcaggt attaatggtc agaggttccc 8280aacaaaggat gttcaaagta tacctgaaat tgatgaaatg tgtgcccgcg ccgtcaagga 8340gaattggcaa actgtgacac cttgcaccct caagaaacag tattgttcta ggcccaaaac 8400caggaccatc ctgggcacta acaacttcat agccttggct catagatcgg cgctcagtgg 8460tgttacccag gcattcatga agaaggcttg gaagtcccca atagccttag ggaaaaacaa 8520attcaaggag ctgcattgca ctgtcgctgg caggtgcctc gaggccgact tggcttcctg 8580tgaccgcagc acccctgcca ttgtgaggtg gttcactacc cacctcctat atgaacttgc 8640aggatgtgaa gaatatctac ctagctatgt gcttaactgt tgccatgacc ttgtggcgac 8700gcaggatggt gctttcacaa aacgcggtgg cctgtcgtct ggagacccag tcaccagtgt 8760gtccaacact gtgtactcac tggtgattta tgcccagcac atggtactat ctgccctgaa 8820aatgggtcat gaaattggcc tcaagttcct cgaagaacaa ctcaaatttg aggaccttct 8880tgaaatccag cctatgttag tatactctga tgatcttgtc ttgtacgcag aaaagcccac 8940cttccccaac tatcattggt gggtcgagca tcttgacctg atgttgggct ttaaaacgga 9000cccaaagaaa accgtcataa ctgataaacc cagtttcctc gggtgcagaa tcgaagcagg 9060gcgacagcta gtccccaatc gcgaccgcat cctggctgct cttgcatatc acatgaaggc 9120gcagaacgcc tcagagtatt atgcgtctgc tgccgcaatc ctgatggatt catgtgcttg 9180cattgaccac gatcctgaat ggtatgagga cctcatctgt ggcattgccc gatgcgctcg

9240cctggacggt tatagctttc caggtccggc atttttcatg tccatgtggg agaagctgag 9300gagtcataat gaagggaaga aattccgcca ctgcggcatc tgcgacgcca aagccgacta 9360cgcggctgct tgtgggcttg atttgtgttt gtttcattcg cactttcacc aacactgccc 9420tgtcactctg agctgcggtc accatgccgg ttcgaaggaa tgttcgcagt gtcagtcacc 9480tgttggggcc ggcagatccc ctcttgatgc tgtgctggaa caaattccat acaaacctcc 9540tcgcactgtc atcatgaagg tgggtaataa aacaacggcc cttgatccgg ggaggtacca 9600gtcccgtcga ggtctcgttg cagtcaagag gggtattgcg ggtaatgaag ttgatcttgc 9660tgatggagat taccaagtgg tacctcttct gccgacttgc aaagatataa acatggtgaa 9720ggtggcttgc aacgtactac tcagcaagtt catagtaggg ccaccaggtt ccgggaagac 9780cacctggcta ctaagtcaag ttcaggacga tgatgtcatt tacacaccca ctcatcagac 9840catgtttgac atagttagtg ctctcaaagt ttgcaggtat tccattccag gagcctcggg 9900actccctttc ccaccgcctg ccaggtccgg gccgtgggtt aggctcattg ccagcgggca 9960cgtccctggc cgagtatcat acctcgatga ggccggatat tgcaatcatc tggacattct 10020tagactgctt tccaaaacac cccttgtgtg tttgggtgac cttcagcaac ttcacccagt 10080cggctttgat tcctattgtt atgtgttcga tcagatgcct cagaagcagt tgaccaccat 10140ttacagattt ggccccaaca tctgcgcagc catccagcct tgttacaggg agaaacttga 10200atctaaggct aggaacacca gggtggtttt taccacccgg cctgtggcct ttggccaggt 10260gctgacacca tatcacaaag atcgcgtcgg ctccgcgatt accatagact catcccaggg 10320ggccaccttt gacattgtaa cattgcatct accatcgcca aagtccctaa ataagtcccg 10380ggcacttgtg gccatcacac gggcaagaca cgggttgttc atttatgacc ctcacaacca 10440gctccgggag tttttcaacc taacccctga gcgcactgat tgtaaccttg tgttcagccg 10500tggagatgag ctggtggtcc tgaatgcaga taatgcagtc acaaccgtgg cgaaggcctt 10560agagacaggt ccaactcaat ttcgagtgtc agacccgagg tgcaagtctc tcttagccgc 10620ttgctcggcc agtctggaag ggagctgcat gccgctaccg caagtggcgc ataacctggg 10680gttttacttc tccccagaca gtccagtatt tgcacctctg ccaaaagagt tggcgccaca 10740ttggccagtg gttacccatc agaataatcg ggcgtggcct gatcgacttg tcgctagtat 10800gcgtccaatt gacgcccgct acagcaagcc gatggtcggt gcagggtatg tggtcggacc 10860gtccaccttc cttggtactc ctggagtggt gtcatactat ctcacactat acatcagggg 10920tgagccccag gccttgccag aaacacttgt ttcaacagga cgtatagcca cagattgtcg 10980ggagtatctc gacgcggctg aggaagaggc agcaaaagaa cttccccacg cgttcattgg 11040cgatgtcaaa ggcaccacag ttggggggtg tcatcacatt acatcaaaat acctacctag 11100gtccctgcct aaagactctg ttgccgtagt tggagtaagt tcgcctggca gggctgctaa 11160agccgtatgc accctcaccg atgtgtacct ccctgaactc cggccatatc tgcaacctga 11220gacggcatca aaatgctgga aactcaaatt agacttcagg gacgtccgac taatggtctg 11280gaaaggagcc accgcctact ttcaattgga agggctcaca tggtcggcgc tgcctgacta 11340tgccaggttt attcagctgc ccaaaaacgc tgttgtatac atcgatccgt gcataggacc 11400ggcaacagcc aatcgtaaag tcgtacgaac cacagattgg cgggccgacc tggcagtgac 11460gccgtatgat tacggtgccc ggaacatttt gacaacagcc tggttcgagg acctcgggcc 11520gcagtggaag attctggggt tgcagccctt taggcgggcg tttggctttg aaaacactga 11580ggattgggca atccttgcat gctgcatgag tgacggcaag gactacactg actataactg 11640gaattgcgtt cgacaacgcc cacacgctat ccatggacgc gctcgtgacc atacgtacca 11700ctttgcccct ggcactgaat tgcaagtgga gctcggtaaa ccccggctgc cacctgagca 11760agtaccgtga attcggagtg atgcaatggg gtcactgtgg agtaaaatca gccagctgtt 11820cgtggacgcc ttcactgaat tccttgttag tgtggttgat attgtcatct tccttgccat 11880attgtttggg ttcaccgtcg caggatggtt actggtcttt cttctcagag tggtttgctc 11940cgcgcttctc cgttcgcgct ctgccattca ctcttccgaa ctatcgaagg tcctatgagg 12000gcttactacc taattgcaga ccggatgttc cacaatttgc atttaagcac cctttgggta 12060tgttttggca catgcgggtt tcccacctaa ttgatcagat ggtctctcgc cgcatctacc 12120agaccatgga acattcaggt caagcggcct ggaagcacgt ggtcagtgag gctactctta 12180caaaattgtc agaactcgac atagttctcc acttccaaca cctggccgca gtggaggcgg 12240actcttgtcg cttcctcagc tcacgacttg tgatgctgaa aaatcttgcc gttggcaatg 12300tgagcttgca gtacaacacc acgttgaacc gcgttgagct catcctcccc acaccaggta 12360cgaggcccaa attgaccgat ttcagacaat ggctcatcag tgtgcacgct tccatttttt 12420cctctgtagc ctcatcagtt actttgttca tagtgctttg gcttcgaatt ccagccgtac 12480gctatgtttt tggtttccat tggcccatgg caacacgtca ttcgagctga ccattaatta 12540cactatatgc atgccctgtc ttaccagcca agcggctcaa caaaggctcg aacccggtcg 12600taacatgtgg tgcaaaatag gacacaccac gtgcgaggag cgtgaccatg atgagttgtc 12660aatgtccatc ccgtccgggt acgacaacct caaacttgaa ggttattacg cttggctggc 12720ttttttgtcc ttttcctacg cggcccaatt ccatccggag ttgtttggaa tagggaatgt 12780gtcgcgcgtc tttgtggata aacgacacca gttcatttgt gccgagcatg acggagataa 12840ttcaaccgta tctaccggac acaacatctc cgcatcatat gcggcatatt atcaccacca 12900aatagacggg ggcaattggt tccatttgga atggctgcgg ccgctctttt cctcttggct 12960agtgctcaac atatcatggt ttctgaggcg ttcgcctgca agccctgttt ctcgacgcat 13020ctatcagata ttaagaccaa tacgaccgcg gctgccggtt tcatggtcct tcaagacatc 13080agttgcctcc gacctcacag ggtctcagca tcgcaagaga acattccctt cggaaagtcg 13140tcacaatgtc gtgaagccgt cggtactccc cagtacatta cgatgactgc taatgtgacc 13200gacgaatcat atttgtacaa cgcggacttg ctaatgcttt ccgcgtgcct tttccacgcc 13260tcagaaatga gcgagaaagg cttcaaagtt atctttggaa acgtctccgg cgttgtttca 13320gcttgtgtca atttcacaga ttatgtggcc catgtaaccc aacataccca acagcatcat 13380ctggtaattg atcacattcg gttactgcat ttcctgacac catctgcaat gaggtgggct 13440acaaccattg cttgtttgtt cgccattctc ttagcgatat gagatgttct cacaaattgg 13500ggcgtttctt gactccgcac tcttgcttct ggtggctttt tttgctgtgt accggcttgt 13560cctggtcctt tgccgatggc aacggcaaca gctcgacacg ccaatacata tataacttga 13620cgatatgcga gctgaatggg accgtctggt tgtccagtca ttttgattgg gcagtcgaga 13680cctttgtgct ttacccggtg gccactcata tcctctcact gggttttctc acaacaagcc 13740atttttttga tgcgctcggt ctcggcgctg tgtccactac gggatttctt ggcgggcggt 13800atgtacttag cagcgtgtac ggcgcctgcg ccttcgcagc gcttgtatgt tttgtcatcc 13860gtgctgctaa aaattgcatg gcttgccgtt atgcccgcac ccggttcacc aacttcatcg 13920tggacgaccg ggggaagatc catcgatgga agtccccaat agtggtagag aaattaggca 13980aagctgacat cggcggcgac cttgtcacca tcaaacatgt tgtcctcgaa ggagtcaaag 14040ctcaaccttt gacgaggaca tcggcggagc aatgggaagc ctagatgatt tttgcaatga 14100tcctaccgcc gcacagaagc ttgtgctggc atttagtatc acatacacac ctataatgat 14160atacgccctc aaggtgtcac gcggccggct cctaggactg ttacacatcc tgatatttct 14220gaactgttct ttcacgttcg gatacatgac atacgtgcac tttcaatcca ctaaccgtgt 14280cgcgcttact atgggggcgg tcgttgccct tttgtggggc atttacagct ttatagaatc 14340atggaagttt gtcacttcca gatgcaggtt gtgttgccta ggccggcgat acattctggc 14400ccctgcccac cacgtagaaa gtgctgcagg cctccattca atcccagcgt ctggtaaccg 14460agcatacgct gtgagaaagc ccggactaac atcagtgaac ggcactctag taccaggact 14520tcggagcctc gtgttgggcg gcaaacgagc tgttaaacga ggagtggtta acctcgtcaa 14580gtatggccgg taaaaaccag agccagaaga aaaagaaaaa cacagctcct atggggagtg 14640gccagccagt caatcaactg tgccaattgc tgggcacaat gataaagtcc cagcgccagc 14700ggcctagggg aggacaggcc aaaatgaaaa agcctgagaa gccacatttc cccctagctg 14760ctgaagatga catccggcac catttcaccc agaccgagcg ttccctttgc ttgcaatcga 14820tccagacggc cttcaatcaa ggcgcaggaa ctgcgtcgct ttcatccagc gggaaggtca 14880gttttcaggt tgagttcatg ctgccggtcg ctcatacggt gcgcctgatt cgcgtaactt 14940ccacatccgc cagtcagggt gcaagttaat ttgatagtta ggtgaatggc cgcgattggc 15000gtgtggcctc tgagtcacct attcaattag ggcgatcaca tgggggttag acttaattgg 15060cgagaaccat gtgaccgaaa ttaaaaaaaa aaaaaaaaaa aaaaaaaaaa 151103215107DNAPorcine reproductive and respiratory syndrome virus 32atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacggtat gatgtggacg ccggaatccg 840atgattcagc cgacctagag gcgctaccgc ctgagctaga acgtcaggtc gaaatcctca 900ttcggagttt tcctgcccat caccctgtca gcctggccga ctgggagctc gctgagtccc 960ctgagaacgg tttttccttc agcacgtacc attctggtgg ttatcttgtc caaaaccccg 1020acgtgtttga cagcaagtgc tggctctcct gtttcttgga tcagccgatc gaagtgcgcc 1080tctatgagga ttatctggct aacgctttcg gttaccaaac caagtggggc gtgtctggta 1140agtacctcca gcgcaggctt caagtcaacg gtattcgtgc tgtaatcgat cctgatggcc 1200ccattcacgt tgaagcgctg tcttgccccc aatcttggat caggcacctg actctggacg 1260atgacgtcac cccaggattc gttcgcctaa catccctccg cattgtgccg aacacagagc 1320ccactactct ccgaatcttt cggtttggag cgcataagtg gtatggcgct gccggcaaac 1380gggctcgtgc caagcgtgcc gctaaaagtg agaagggtcc ggctcccact cccaaggttg 1440ccccgccagc ccccacctgc ggaattgtta cctactctcc accaacagac gggtcttgcg 1500gttggcacgt ccttgccgcc ataatgaacc gaatgatgaa cggtgacttc atgtcccctc 1560tggcccagta caacagacca gaggatgatt gggcttctga ttacgatctt gcccaggcga 1620ttcaatgtct gcggttgcct gctaccatag ttcggaatcg tgcctgtccc aacgccaagt 1680acctcataaa actcaacgga gtccactggg aggtagaggt gaggtcaggg atggcccctc 1740gctcccttcc ccgcgagtgc gtagtcggcg tttgttccga aggctgtgcc gcatcgcctt 1800acccagaaaa cgggctacct aaacgagcgt ttgaggcctt ggcgtctgct tacagactac 1860cttccgattg tgtcagttct ggtattgctg actttcttgc taacccccct caggaattct 1920ggactcttga caaaatgttg acctccccgt caccggagcg gtctggtttt tccagtctgt 1980acaaattgct attagaggtt gtcccgcaga aatgcggagc cacggaaggg gctttcacct 2040atgctgttga gagaatgctg aaggattgcc cgagctccga acaggccatg gcccttctgg 2100caaaaattaa agttccatcc tcaaaggccc cgtctgtgtc cctggacgag tgtttcccta 2160cggatgtttc agccgatttc gaaccagcat ctcaggagag gtcccaaaat tccagcgctg 2220ctgttgtcct gcattcaccg aatgcaaaag agttcgagga agcagctcca ggggaagttc 2280aggagggtgg ccacaaggcc gtccactctg cactccctgc cgggggtcct aacaataagc 2340aggcacagct ggttgccggt gagcaactga agctcggcgg ttgtggttcg gtagttggga 2400atgcacatga aggtgttctg gtcccacctg gtccaattaa tttgacaagc ggggatttac 2460cctcctcagg ctccatgaaa gaagatatgc tcaatagccg ggaggacgaa ccactggatt 2520tgtcccaacc agcaacagct gtcacaacga ctcttatggg agagctaaca cccgactacc 2580taggttctga tactggtgcc ctccccgtca ccgtccgaaa atttgtcccg acggggccta 2640tactccgtca tgttgagcac tgcagcacgg ggtcgggcga tagcagttcg cctttggatc 2700tgtctggtgc gcaaaccccg gaccagcctt taaatctgtc cctggcggct tggccagtga 2760ggaccaccgc gtctgatcct ggctgggtcc acggtagacg cgagcctgtc tttgtaaagc 2820ctcgagatgt tttctctgat ggcgattcag cccttcagtt cggggagctt tctgaatcca 2880gctctgtcat cgagtttgat cgggcaaaag atgctcaggt ggctgacgcc cctgtcggtc 2940tgacgacttc ggacgaggcc ctctccgcag tcgatccttt cgagttttcc gaactcaagc 3000gcccacgttt ctccgcacaa gccttaattg accgaggcgg cccacttgcc gatgtccatg 3060caaaaataaa gaaccgggta tatgaacagt gcctccaagc ttgtgagccc ggtagtcgcg 3120caaccccagc taccagggag tggctcgaca aaatgtggga gagagtggac atgaaaacct 3180ggcgctgcac ctcacagttc caagctggtc acattcttgc gtccctcaaa ttcctccctg 3240acatgattca agacacaccg cctcctgttc ccaggaagaa ccgagccagt gataaagctg 3300gcctgaaaca actagtggca cagtgggata ggagattgag ttcaaccccc cccccaaaac 3360cggttgggcc ggtacttgac cgggtcgatc ctccgcctac gggtacccgg caagaagacg 3420tcaccccctc cgatgggcca ccccatgcgc cggatggtcg agtgagtacg ggtgggagtt 3480ggaaaggcct tatgctttcc ggcacccgtc tcacggggtc catcagtcat cgcctcatga 3540catgggtttt tgaagttgtc tcccatctcc cagcttttat gctcacactt ttctcgccgc 3600ggggctctat ggctccaggc gattgggttt ttgcaggtgt tgttttactt gctctcctgc 3660tctgtcgttc ttacccaata ttcgggtgcc ttcccttatt gggtgtcttt tctggtactg 3720tgcggcgtgt tcgtctgggt gtttttggct cttggatggc ttttgctgta tttttattct 3780cgactccatc caacccagtc ggttcttctt gtaaccacga ttcgccggag tgtcatgctg 3840agcttctggc tcttgagcag cgccaacttt gggaacctgt gcgcggcctt gtggtcggcc 3900catcgggcct cttatgtgtc attcttggca agttactcgg tgggtcacgt tatctctggc 3960atgttctcct acgtctatgc ctgcttgcag atttggccct ttctcttgtt tatgtggtgt 4020cccaggggcg ttgtcacaag tgttggggaa agtgtataag gacagctcct gctgaggtgg 4080cttttaatgt atttcctttc tcgcgcgcca cccgtagttc tcttgtatcc ttatgtgatc 4140gattccaaac gccaaaagga gttgatcccg tgcacttggc gacaggttgg cgcgggtgct 4200ggcgtggtga gagtcctatc catcaaccac accaaaagcc catagcttac gccaatttgg 4260atgaaaagaa gatatctgcc caaacggttg ttgctgtccc atacgatccc aatcaggcta 4320tcaaatgtct gaaagttctg caggcgggag gggctatcgt ggatcagcct acgcctgagg 4380tcgttcgtgt gtccgagatc cccttctcag ccccattttt cccaaaagtt ccagtcaacc 4440cagattgtag ggtcgtggtg gattcggaca cttttgtggc tgcggttcgt tgtggttatt 4500cgacatcaca actggtcctg ggccagggca actttgccaa gttaaatcaa acccccccca 4560ggaactctat ctccaccaaa gcgactggtg gggcctctta tacttttgct gtggctcaag 4620tgtctgtgtg gacccttgtt catttcgtcc tcggtctttg gctcacgtca cctcaagtgt 4680gtggtcgagg aaccgctgac ccatggtgtt caagtccatt ttcatatcct acctatggcc 4740ccggggttgt gtgctcctct cgactttgtg tgtctgctga cggggtcacc cttccattgt 4800tctcagccgt ggcacaactc tctggtaggg aggtggggat ttttatttta gtgctcgtct 4860cctttattgc cttggcccac cgcatggctc ttaaggcaga catgttagta gtctttttgg 4920ctctttgtgc ttatgcctgg cccatgagct cctggttgat ctgcttcttt cctatactct 4980tgaggtgggt tacccttcac cctctcacta tgctttgggt gcattcattc ttgatgtttt 5040gtctcccagc agccggcgtc ctctcactgg ggataactgg cctcctctgg gcaatcggcc 5100gctttactca ggttgccgga atcattacac cttatgatat ccaccagtac acctctgggc 5160cacgtggtgc agccgctgtg gccacagccc cagaaggcac ttacatggcc gccgtccgga 5220gagctgcttt aaccgggcgg actttaatct tcaccccgtc cgcagttgga tcccttctcg 5280aaggtgcttt caggactcat aaaccctgcc ttaacaccgt gaatgtcgta ggctcctccc 5340ttggttccgg aggggttttc accattgacg gaaaaaaaat tgtcgtcact gctgcccatg 5400tgctgaacgg cgacacagct agagtcaccg gtgattccta caaccgcatg cacactttca 5460agactaatgg tgactatgcc tggtcccatg ctgataactg gcagggcgct gcccctgtgg 5520tcaaggttgc gaaagggtat cgcggtcgtg cctactggca aacatcaact ggtgtcgagc 5580ctggtgttat tgggaatggg ttcgccttct gtttcaccaa ctgcggcgat tcggggtcac 5640ccgttatctc agaatctggt gatcttatcg gaatccacac cggttcaaac aaacttggtt 5700ctggtcttgt gacaacccct gaaggggaga cctgtaccat cagagaaacc aagctttctg 5760acctttccag acatttcgca ggcccaagcg ttcctcttgg ggacatcaaa ttgagtccgg 5820ccatcatccc tgatgtgaca tccattccga gtgacttggc atcgctccta gcttccgtcc 5880ctgtaatgga aggcggcctc tcgaccgttc aacttttgtg tgtctttttc ctcctctggc 5940gcatgatggg ccatgcctgg acgcccattg ttgccgtggg cttctttttg ctgaatgaaa 6000ttcttccagc agttttggtt cgagccgtgt tttcttttgc actctttgtg cttgcatggg 6060ccaccccctg gtctgcacaa gtgttgatga ttagacttct cacggcatct ctcaaccgca 6120acaaactttc tcttgcgttc tacgcactcg gaggtgttgt tggtttggct gctgaaatcg 6180ggacttttgc tggtaaattg tctgaattgt ctcaagctct ttcgacatac tgtttcttac 6240ctagggtcct tgctatgacc agctgtgttc ccatcatcat cattggtgga ctccatgccc 6300tcggtgtgat tctgtggtta ttcaaatacc ggtgcctcca caacatgctg gttggtgatg 6360gaagcttttc aagcgctttc ttcctacggt attttgcaga gggcaatctc aggagaggtg 6420tttcacagtc ctgtggcatg agtaacgagt ccctgacggc tgctttggct tgcaagttgt 6480cacaggctga ccttgatttt ttgtccagct taacgaactt caagtgcttt gtatctgctt 6540caaacatgaa aaatgctgcc ggccagtaca ttgaagcagc ttatgccagg gccctgcgtc 6600aagagttggc ctctttagtc cagattgaca aaatgaaagg agttttgtcc aagctagagg 6660cctttgctga aacggccact ccgtccctcg acgtaggtga cgtgattgtt ctacttggac 6720aacatcctca cggatccgtt ctcgatatta atgtggggac tgaaaggaaa actgtatccg 6780tgcaagagac ccggagccta ggcggctcca agttcagtgt ttgtactgtt gtgtcaaaca 6840cacccgtgga cgccttagcc ggtattccac tccagacacc aacccccctt ttcgagaatg 6900gcccgcgtca tcgcagcgag gaggacgatc ttaaagtcga gaggatgaag aaacactgcg 6960tgtccctcgg cttccacaac attaacggta aagtttactg caagatttgg gacaagtcta 7020ccggtgacgc cttttacact gatgattccc ggtacaccca agactatgct tttcaggaca 7080ggtcagctga ctatagagac agggactacg agggtgtgca aaccgccccc caacagggat 7140ttgatccaaa gtctgaaacc cctgttggta ccgttgtgat cggcggtatt acgtacaaca 7200ggtatttggt caaaggtaag gaggttctgg ttcccaagcc tgacaactgc cttgaagctg 7260ccaagctgtc ccttgagcaa gctctcgctg ggatgggcca aacttgtgac cttacagctg 7320ccgaggtgga aaagctaaag cgcatcattg gtcaacttca aggattgacc actgagcagg 7380ctttaaactg ttagccgcca gcggcttgac ccgctgtggc cgcggcggcc tagttgtaac 7440tgaaacggcg gtaaaaattg tcaaatacca cagcagaact tttaccttag gctctttaga 7500cctaaaagtc acttccgagg tggaggtgaa gaagtcaacc gagcagggcc acgctgttgt 7560ggcaaacttg tgttctggtg tcgtcttgat gagacctcac ccaccgtccc ttgtcgacgt 7620tcttctgaaa cccggacttg acataacacc cggcattcaa ccagggcatg gggccgggaa 7680tatgggcgtg gacggttcca tttgggattt tgaaaccgca cccacaaagg ctgaactcga 7740gttatccaag caaataattc aagcatgtga agtcaggcgc ggggatgccc cgaacctcca 7800gctcccttac aagctctatc ctgttagagg ggatcctgag cggcataaag gccacctcat 7860caataccagg tttggagact taccttacaa aactcctcaa gacaccaagt ccgcaatcca 7920cgcggcttgt tgcctgcacc ccaacggggc ccccgtgtct gatggtaaat ccacactagg 7980taccactctt caacatggct tcgagcttta tgtccctact gtgccctata gtgtcatgga 8040gtaccttgat tcacgcccag acaccccttt tatgtgcact aaacacggca cttccaaggc 8100tgctgcagag gacctccaaa aatacgacct atccacccaa ggatttgtcc tgcctggggt 8160cctacgccta gtgcgcaggt tcatctttgg ccacattggc aaggcaccgc cattgttcct 8220cccatcaact tatcccgcca agaactccat ggcaggtatt aatggtcaga ggttcccaac 8280aaaggatgtt caaagtatac ctgaaattga tgaaatgtgt gcccgcgccg tcaaggagaa 8340ttggcaaact gtgacacctt gcaccctcaa gaaacagtat tgttctaggc ccaaaaccag 8400gaccatcctg ggcactaaca acttcatagc cttggctcat agatcggcgc tcagtggtgt 8460tacccaggca ttcatgaaga aggcttggaa gtccccaata gccttaggga aaaacaaatt 8520caaggagctg cattgcactg tcgctggcag gtgcctcgag gccgacttgg cttcctgtga 8580ccgcagcacc cctgccattg tgaggtggtt cactacccac ctcctatatg aacttgcagg 8640atgtgaagaa tatctaccta gctatgtgct taactgttgc catgaccttg tggcgacgca 8700ggatggtgct ttcacaaaac gcggtggcct gtcgtctgga gacccagtca ccagtgtgtc 8760caacactgtg tactcactgg tgatttatgc ccagcacatg gtactatctg ccctgaaaat 8820gggtcatgaa attggcctca agttcctcga agaacaactc aaatttgagg accttcttga 8880aatccagcct atgttagtat actctgatga tcttgtcttg tacgcagaaa agcccacctt 8940ccccaactat cattggtggg tcgagcatct tgacctgatg ttgggcttta aaacggaccc 9000aaagaaaacc gtcataactg ataaacccag tttcctcggg tgcagaatcg aagcagggcg 9060acagctagtc cccaatcgcg accgcatcct ggctgctctt gcatatcaca tgaaggcgca

9120gaacgcctca gagtattatg cgtctgctgc cgcaatcctg atggattcat gtgcttgcat 9180tgaccacgat cctgaatggt atgaggacct catctgtggc attgcccgat gcgctcgcct 9240ggacggttat agctttccag gtccggcatt tttcatgtcc atgtgggaga agctgaggag 9300tcataatgaa gggaagaaat tccgccactg cggcatctgc gacgccaaag ccgactacgc 9360ggctgcttgt gggcttgatt tgtgtttgtt tcattcgcac tttcaccaac actgccctgt 9420cactctgagc tgcggtcacc atgccggttc gaaggaatgt tcgcagtgtc agtcacctgt 9480tggggccggc agatcccctc ttgatgctgt gctggaacaa attccataca aacctcctcg 9540cactgtcatc atgaaggtgg gtaataaaac aacggccctt gatccgggga ggtaccagtc 9600ccgtcgaggt ctcgttgcag tcaagagggg tattgcgggt aatgaagttg atcttgctga 9660tggagattac caagtggtac ctcttctgcc gacttgcaaa gatataaaca tggtgaaggt 9720ggcttgcaac gtactactca gcaagttcat agtagggcca ccaggttccg ggaagaccac 9780ctggctacta agtcaagttc aggacgatga tgtcatttac acacccactc atcagaccat 9840gtttgacata gttagtgctc tcaaagtttg caggtattcc attccaggag cctcgggact 9900ccctttccca ccgcctgcca ggtccgggcc gtgggttagg ctcattgcca gcgggcacgt 9960ccctggccga gtatcatacc tcgatgaggc cggatattgc aatcatctgg acattcttag 10020actgctttcc aaaacacccc ttgtgtgttt gggtgacctt cagcaacttc acccagtcgg 10080ctttgattcc tattgttatg tgttcgatca gatgcctcag aagcagttga ccaccattta 10140cagatttggc cccaacatct gcgcagccat ccagccttgt tacagggaga aacttgaatc 10200taaggctagg aacaccaggg tggtttttac cacccggcct gtggcctttg gccaggtgct 10260gacaccatat cacaaagatc gcgtcggctc cgcgattacc atagactcat cccagggggc 10320cacctttgac attgtaacat tgcatctacc atcgccaaag tccctaaata agtcccgggc 10380acttgtggcc atcacacggg caagacacgg gttgttcatt tatgaccctc acaaccagct 10440ccgggagttt ttcaacctaa cccctgagcg cactgattgt aaccttgtgt tcagccgtgg 10500agatgagctg gtggtcctga atgcagataa tgcagtcaca accgtggcga aggccttaga 10560gacaggtcca actcaatttc gagtgtcaga cccgaggtgc aagtctctct tagccgcttg 10620ctcggccagt ctggaaggga gctgcatgcc gctaccgcaa gtggcgcata acctggggtt 10680ttacttctcc ccagacagtc cagtatttgc acctctgcca aaagagttgg cgccacattg 10740gccagtggtt acccatcaga ataatcgggc gtggcctgat cgacttgtcg ctagtatgcg 10800tccaattgac gcccgctaca gcaagccgat ggtcggtgca gggtatgtgg tcggaccgtc 10860caccttcctt ggtactcctg gagtggtgtc atactatctc acactataca tcaggggtga 10920gccccaggcc ttgccagaaa cacttgtttc aacaggacgt atagccacag attgtcggga 10980gtatctcgac gcggctgagg aagaggcagc aaaagaactt ccccacgcgt tcattggcga 11040tgtcaaaggc accacagttg gggggtgtca tcacattaca tcaaaatacc tacctaggtc 11100cctgcctaaa gactctgttg ccgtagttgg agtaagttcg cctggcaggg ctgctaaagc 11160cgtatgcacc ctcaccgatg tgtacctccc tgaactccgg ccatatctgc aacctgagac 11220ggcatcaaaa tgctggaaac tcaaattaga cttcagggac gtccgactaa tggtctggaa 11280aggagccacc gcctactttc aattggaagg gctcacatgg tcggcgctgc ctgactatgc 11340caggtttatt cagctgccca aaaacgctgt tgtatacatc gatccgtgca taggaccggc 11400aacagccaat cgtaaagtcg tacgaaccac agattggcgg gccgacctgg cagtgacgcc 11460gtatgattac ggtgcccgga acattttgac aacagcctgg ttcgaggacc tcgggccgca 11520gtggaagatt ctggggttgc agccctttag gcgggcgttt ggctttgaaa acactgagga 11580ttgggcaatc cttgcatgct gcatgagtga cggcaaggac tacactgact ataactggaa 11640ttgcgttcga caacgcccac acgctatcca tggacgcgct cgtgaccata cgtaccactt 11700tgcccctggc actgaattgc aagtggagct cggtaaaccc cggctgccac ctgagcaagt 11760accgtgaatt cggagtgatg caatggggtc actgtggagt aaaatcagcc agctgttcgt 11820ggacgccttc actgaattcc ttgttagtgt ggttgatatt gtcatcttcc ttgccatatt 11880gtttgggttc accgtcgcag gatggttact ggtctttctt ctcagagtgg tttgctccgc 11940gcttctccgt tcgcgctctg ccattcactc ttccgaacta tcgaaggtcc tatgagggct 12000tactacctaa ttgcagaccg gatgttccac aatttgcatt taagcaccct ttgggtatgt 12060tttggcacat gcgggtttcc cacctaattg atcagatggt ctctcgccgc atctaccaga 12120ccatggaaca ttcaggtcaa gcggcctgga agcacgtggt cagtgaggct actcttacaa 12180aattgtcaga actcgacata gttctccact tccaacacct ggccgcagtg gaggcggact 12240cttgtcgctt cctcagctca cgacttgtga tgctgaaaaa tcttgccgtt ggcaatgtga 12300gcttgcagta caacaccacg ttgaaccgcg ttgagctcat cctccccaca ccaggtacga 12360ggcccaaatt gaccgatttc agacaatggc tcatcagtgt gcacgcttcc attttttcct 12420ctgtagcctc atcagttact ttgttcatag tgctttggct tcgaattcca gccgtacgct 12480atgtttttgg tttccattgg cccatggcaa cacgtcattc gagctgacca ttaattacac 12540tatatgcatg ccctgtctta ccagccaagc ggctcaacaa aggctcgaac ccggtcgtaa 12600catgtggtgc aaaataggac acaccacgtg cgaggagcgt gaccatgatg agttgtcaat 12660gtccatcccg tccgggtacg acaacctcaa acttgaaggt tattacgctt ggctggcttt 12720tttgtccttt tcctacgcgg cccaattcca tccggagttg tttggaatag ggaatgtgtc 12780gcgcgtcttt gtggataaac gacaccagtt catttgtgcc gagcatgacg gagataattc 12840aaccgtatct accggacaca acatctccgc atcatatgcg gcatattatc accaccaaat 12900agacgggggc aattggttcc atttggaatg gctgcggccg ctcttttcct cttggctagt 12960gctcaacata tcatggtttc tgaggcgttc gcctgcaagc cctgtttctc gacgcatcta 13020tcagatatta agaccaatac gaccgcggct gccggtttca tggtccttca agacatcagt 13080tgcctccgac ctcacagggt ctcagcatcg caagagaaca ttcccttcgg aaagtcgtca 13140caatgtcgtg aagccgtcgg tactccccag tacattacga tgactgctaa tgtgaccgac 13200gaatcatatt tgtacaacgc ggacttgcta atgctttccg cgtgcctttt ccacgcctca 13260gaaatgagcg agaaaggctt caaagttatc tttggaaacg tctccggcgt tgtttcagct 13320tgtgtcaatt tcacagatta tgtggcccat gtaacccaac atacccaaca gcatcatctg 13380gtaattgatc acattcggtt actgcatttc ctgacaccat ctgcaatgag gtgggctaca 13440accattgctt gtttgttcgc cattctctta gcgatatgag atgttctcac aaattggggc 13500gtttcttgac tccgcactct tgcttctggt ggcttttttt gctgtgtacc ggcttgtcct 13560ggtcctttgc cgatggcaac ggcaacagct cgacacgcca atacatatat aacttgacga 13620tatgcgagct gaatgggacc gtctggttgt ccagtcattt tgattgggca gtcgagacct 13680ttgtgcttta cccggtggcc actcatatcc tctcactggg ttttctcaca acaagccatt 13740tttttgatgc gctcggtctc ggcgctgtgt ccactacggg atttcttggc gggcggtatg 13800tacttagcag cgtgtacggc gcctgcgcct tcgcagcgct tgtatgtttt gtcatccgtg 13860ctgctaaaaa ttgcatggct tgccgttatg cccgcacccg gttcaccaac ttcatcgtgg 13920acgaccgggg gaagatccat cgatggaagt ccccaatagt ggtagagaaa ttaggcaaag 13980ctgacatcgg cggcgacctt gtcaccatca aacatgttgt cctcgaagga gtcaaagctc 14040aacctttgac gaggacatcg gcggagcaat gggaagccta gatgattttt gcaatgatcc 14100taccgccgca cagaagcttg tgctggcatt tagtatcaca tacacaccta taatgatata 14160cgccctcaag gtgtcacgcg gccggctcct aggactgtta cacatcctga tatttctgaa 14220ctgttctttc acgttcggat acatgacata cgtgcacttt caatccacta accgtgtcgc 14280gcttactatg ggggcggtcg ttgccctttt gtggggcatt tacagcttta tagaatcatg 14340gaagtttgtc acttccagat gcaggttgtg ttgcctaggc cggcgataca ttctggcccc 14400tgcccaccac gtagaaagtg ctgcaggcct ccattcaatc ccagcgtctg gtaaccgagc 14460atacgctgtg agaaagcccg gactaacatc agtgaacggc actctagtac caggacttcg 14520gagcctcgtg ttgggcggca aacgagctgt taaacgagga gtggttaacc tcgtcaagta 14580tggccggtaa aaaccagagc cagaagaaaa agaaaaacac agctcctatg gggagtggcc 14640agccagtcaa tcaactgtgc caattgctgg gcacaatgat aaagtcccag cgccagcggc 14700ctaggggagg acaggccaaa atgaaaaagc ctgagaagcc acatttcccc ctagctgctg 14760aagatgacat ccggcaccat ttcacccaga ccgagcgttc cctttgcttg caatcgatcc 14820agacggcctt caatcaaggc gcaggaactg cgtcgctttc atccagcggg aaggtcagtt 14880ttcaggttga gttcatgctg ccggtcgctc atacggtgcg cctgattcgc gtaacttcca 14940catccgccag tcagggtgca agttaatttg atagttaggt gaatggccgc gattggcgtg 15000tggcctctga gtcacctatt caattagggc gatcacatgg gggttagact taattggcga 15060gaaccatgtg accgaaatta aaaaaaaaaa aaaaaaaaaa aaaaaaa 151073315104DNAPorcine reproductive and respiratory syndrome virus 33atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccaacatgat gtggacgccg gaatccgatg 840attcagccga cctagaggcg ctaccgcctg agctagaacg tcaggtcgaa atcctcattc 900ggagttttcc tgcccatcac cctgtcagcc tggccgactg ggagctcgct gagtcccctg 960agaacggttt ttccttcagc acgtaccatt ctggtggtta tcttgtccaa aaccccgacg 1020tgtttgacag caagtgctgg ctctcctgtt tcttggatca gccgatcgaa gtgcgcctct 1080atgaggatta tctggctaac gctttcggtt accaaaccaa gtggggcgtg tctggtaagt 1140acctccagcg caggcttcaa gtcaacggta ttcgtgctgt aatcgatcct gatggcccca 1200ttcacgttga agcgctgtct tgcccccaat cttggatcag gcacctgact ctggacgatg 1260acgtcacccc aggattcgtt cgcctaacat ccctccgcat tgtgccgaac acagagccca 1320ctactctccg aatctttcgg tttggagcgc ataagtggta tggcgctgcc ggcaaacggg 1380ctcgtgccaa gcgtgccgct aaaagtgaga agggtccggc tcccactccc aaggttgccc 1440cgccagcccc cacctgcgga attgttacct actctccacc aacagacggg tcttgcggtt 1500ggcacgtcct tgccgccata atgaaccgaa tgatgaacgg tgacttcatg tcccctctgg 1560cccagtacaa cagaccagag gatgattggg cttctgatta cgatcttgcc caggcgattc 1620aatgtctgcg gttgcctgct accatagttc ggaatcgtgc ctgtcccaac gccaagtacc 1680tcataaaact caacggagtc cactgggagg tagaggtgag gtcagggatg gcccctcgct 1740cccttccccg cgagtgcgta gtcggcgttt gttccgaagg ctgtgccgca tcgccttacc 1800cagaaaacgg gctacctaaa cgagcgtttg aggccttggc gtctgcttac agactacctt 1860ccgattgtgt cagttctggt attgctgact ttcttgctaa cccccctcag gaattctgga 1920ctcttgacaa aatgttgacc tccccgtcac cggagcggtc tggtttttcc agtctgtaca 1980aattgctatt agaggttgtc ccgcagaaat gcggagccac ggaaggggct ttcacctatg 2040ctgttgagag aatgctgaag gattgcccga gctccgaaca ggccatggcc cttctggcaa 2100aaattaaagt tccatcctca aaggccccgt ctgtgtccct ggacgagtgt ttccctacgg 2160atgtttcagc cgatttcgaa ccagcatctc aggagaggtc ccaaaattcc agcgctgctg 2220ttgtcctgca ttcaccgaat gcaaaagagt tcgaggaagc agctccaggg gaagttcagg 2280agggtggcca caaggccgtc cactctgcac tccctgccgg gggtcctaac aataagcagg 2340cacagctggt tgccggtgag caactgaagc tcggcggttg tggttcggta gttgggaatg 2400cacatgaagg tgttctggtc ccacctggtc caattaattt gacaagcggg gatttaccct 2460cctcaggctc catgaaagaa gatatgctca atagccggga ggacgaacca ctggatttgt 2520cccaaccagc aacagctgtc acaacgactc ttatgggaga gctaacaccc gactacctag 2580gttctgatac tggtgccctc cccgtcaccg tccgaaaatt tgtcccgacg gggcctatac 2640tccgtcatgt tgagcactgc agcacggggt cgggcgatag cagttcgcct ttggatctgt 2700ctggtgcgca aaccccggac cagcctttaa atctgtccct ggcggcttgg ccagtgagga 2760ccaccgcgtc tgatcctggc tgggtccacg gtagacgcga gcctgtcttt gtaaagcctc 2820gagatgtttt ctctgatggc gattcagccc ttcagttcgg ggagctttct gaatccagct 2880ctgtcatcga gtttgatcgg gcaaaagatg ctcaggtggc tgacgcccct gtcggtctga 2940cgacttcgga cgaggccctc tccgcagtcg atcctttcga gttttccgaa ctcaagcgcc 3000cacgtttctc cgcacaagcc ttaattgacc gaggcggccc acttgccgat gtccatgcaa 3060aaataaagaa ccgggtatat gaacagtgcc tccaagcttg tgagcccggt agtcgcgcaa 3120ccccagctac cagggagtgg ctcgacaaaa tgtgggagag agtggacatg aaaacctggc 3180gctgcacctc acagttccaa gctggtcaca ttcttgcgtc cctcaaattc ctccctgaca 3240tgattcaaga cacaccgcct cctgttccca ggaagaaccg agccagtgat aaagctggcc 3300tgaaacaact agtggcacag tgggatagga gattgagttc aacccccccc ccaaaaccgg 3360ttgggccggt acttgaccgg gtcgatcctc cgcctacggg tacccggcaa gaagacgtca 3420ccccctccga tgggccaccc catgcgccgg atggtcgagt gagtacgggt gggagttgga 3480aaggccttat gctttccggc acccgtctca cggggtccat cagtcatcgc ctcatgacat 3540gggtttttga agttgtctcc catctcccag cttttatgct cacacttttc tcgccgcggg 3600gctctatggc tccaggcgat tgggtttttg caggtgttgt tttacttgct ctcctgctct 3660gtcgttctta cccaatattc gggtgccttc ccttattggg tgtcttttct ggtactgtgc 3720ggcgtgttcg tctgggtgtt tttggctctt ggatggcttt tgctgtattt ttattctcga 3780ctccatccaa cccagtcggt tcttcttgta accacgattc gccggagtgt catgctgagc 3840ttctggctct tgagcagcgc caactttggg aacctgtgcg cggccttgtg gtcggcccat 3900cgggcctctt atgtgtcatt cttggcaagt tactcggtgg gtcacgttat ctctggcatg 3960ttctcctacg tctatgcctg cttgcagatt tggccctttc tcttgtttat gtggtgtccc 4020aggggcgttg tcacaagtgt tggggaaagt gtataaggac agctcctgct gaggtggctt 4080ttaatgtatt tcctttctcg cgcgccaccc gtagttctct tgtatcctta tgtgatcgat 4140tccaaacgcc aaaaggagtt gatcccgtgc acttggcgac aggttggcgc gggtgctggc 4200gtggtgagag tcctatccat caaccacacc aaaagcccat agcttacgcc aatttggatg 4260aaaagaagat atctgcccaa acggttgttg ctgtcccata cgatcccaat caggctatca 4320aatgtctgaa agttctgcag gcgggagggg ctatcgtgga tcagcctacg cctgaggtcg 4380ttcgtgtgtc cgagatcccc ttctcagccc catttttccc aaaagttcca gtcaacccag 4440attgtagggt cgtggtggat tcggacactt ttgtggctgc ggttcgttgt ggttattcga 4500catcacaact ggtcctgggc cagggcaact ttgccaagtt aaatcaaacc ccccccagga 4560actctatctc caccaaagcg actggtgggg cctcttatac ttttgctgtg gctcaagtgt 4620ctgtgtggac ccttgttcat ttcgtcctcg gtctttggct cacgtcacct caagtgtgtg 4680gtcgaggaac cgctgaccca tggtgttcaa gtccattttc atatcctacc tatggccccg 4740gggttgtgtg ctcctctcga ctttgtgtgt ctgctgacgg ggtcaccctt ccattgttct 4800cagccgtggc acaactctct ggtagggagg tggggatttt tattttagtg ctcgtctcct 4860ttattgcctt ggcccaccgc atggctctta aggcagacat gttagtagtc tttttggctc 4920tttgtgctta tgcctggccc atgagctcct ggttgatctg cttctttcct atactcttga 4980ggtgggttac ccttcaccct ctcactatgc tttgggtgca ttcattcttg atgttttgtc 5040tcccagcagc cggcgtcctc tcactgggga taactggcct cctctgggca atcggccgct 5100ttactcaggt tgccggaatc attacacctt atgatatcca ccagtacacc tctgggccac 5160gtggtgcagc cgctgtggcc acagccccag aaggcactta catggccgcc gtccggagag 5220ctgctttaac cgggcggact ttaatcttca ccccgtccgc agttggatcc cttctcgaag 5280gtgctttcag gactcataaa ccctgcctta acaccgtgaa tgtcgtaggc tcctcccttg 5340gttccggagg ggttttcacc attgacggaa aaaaaattgt cgtcactgct gcccatgtgc 5400tgaacggcga cacagctaga gtcaccggtg attcctacaa ccgcatgcac actttcaaga 5460ctaatggtga ctatgcctgg tcccatgctg ataactggca gggcgctgcc cctgtggtca 5520aggttgcgaa agggtatcgc ggtcgtgcct actggcaaac atcaactggt gtcgagcctg 5580gtgttattgg gaatgggttc gccttctgtt tcaccaactg cggcgattcg gggtcacccg 5640ttatctcaga atctggtgat cttatcggaa tccacaccgg ttcaaacaaa cttggttctg 5700gtcttgtgac aacccctgaa ggggagacct gtaccatcag agaaaccaag ctttctgacc 5760tttccagaca tttcgcaggc ccaagcgttc ctcttgggga catcaaattg agtccggcca 5820tcatccctga tgtgacatcc attccgagtg acttggcatc gctcctagct tccgtccctg 5880taatggaagg cggcctctcg accgttcaac ttttgtgtgt ctttttcctc ctctggcgca 5940tgatgggcca tgcctggacg cccattgttg ccgtgggctt ctttttgctg aatgaaattc 6000ttccagcagt tttggttcga gccgtgtttt cttttgcact ctttgtgctt gcatgggcca 6060ccccctggtc tgcacaagtg ttgatgatta gacttctcac ggcatctctc aaccgcaaca 6120aactttctct tgcgttctac gcactcggag gtgttgttgg tttggctgct gaaatcggga 6180cttttgctgg taaattgtct gaattgtctc aagctctttc gacatactgt ttcttaccta 6240gggtccttgc tatgaccagc tgtgttccca tcatcatcat tggtggactc catgccctcg 6300gtgtgattct gtggttattc aaataccggt gcctccacaa catgctggtt ggtgatggaa 6360gcttttcaag cgctttcttc ctacggtatt ttgcagaggg caatctcagg agaggtgttt 6420cacagtcctg tggcatgagt aacgagtccc tgacggctgc tttggcttgc aagttgtcac 6480aggctgacct tgattttttg tccagcttaa cgaacttcaa gtgctttgta tctgcttcaa 6540acatgaaaaa tgctgccggc cagtacattg aagcagctta tgccagggcc ctgcgtcaag 6600agttggcctc tttagtccag attgacaaaa tgaaaggagt tttgtccaag ctagaggcct 6660ttgctgaaac ggccactccg tccctcgacg taggtgacgt gattgttcta cttggacaac 6720atcctcacgg atccgttctc gatattaatg tggggactga aaggaaaact gtatccgtgc 6780aagagacccg gagcctaggc ggctccaagt tcagtgtttg tactgttgtg tcaaacacac 6840ccgtggacgc cttagccggt attccactcc agacaccaac cccccttttc gagaatggcc 6900cgcgtcatcg cagcgaggag gacgatctta aagtcgagag gatgaagaaa cactgcgtgt 6960ccctcggctt ccacaacatt aacggtaaag tttactgcaa gatttgggac aagtctaccg 7020gtgacgcctt ttacactgat gattcccggt acacccaaga ctatgctttt caggacaggt 7080cagctgacta tagagacagg gactacgagg gtgtgcaaac cgccccccaa cagggatttg 7140atccaaagtc tgaaacccct gttggtaccg ttgtgatcgg cggtattacg tacaacaggt 7200atttggtcaa aggtaaggag gttctggttc ccaagcctga caactgcctt gaagctgcca 7260agctgtccct tgagcaagct ctcgctggga tgggccaaac ttgtgacctt acagctgccg 7320aggtggaaaa gctaaagcgc atcattggtc aacttcaagg attgaccact gagcaggctt 7380taaactgtta gccgccagcg gcttgacccg ctgtggccgc ggcggcctag ttgtaactga 7440aacggcggta aaaattgtca aataccacag cagaactttt accttaggct ctttagacct 7500aaaagtcact tccgaggtgg aggtgaagaa gtcaaccgag cagggccacg ctgttgtggc 7560aaacttgtgt tctggtgtcg tcttgatgag acctcaccca ccgtcccttg tcgacgttct 7620tctgaaaccc ggacttgaca taacacccgg cattcaacca gggcatgggg ccgggaatat 7680gggcgtggac ggttccattt gggattttga aaccgcaccc acaaaggctg aactcgagtt 7740atccaagcaa ataattcaag catgtgaagt caggcgcggg gatgccccga acctccagct 7800cccttacaag ctctatcctg ttagagggga tcctgagcgg cataaaggcc acctcatcaa 7860taccaggttt ggagacttac cttacaaaac tcctcaagac accaagtccg caatccacgc 7920ggcttgttgc ctgcacccca acggggcccc cgtgtctgat ggtaaatcca cactaggtac 7980cactcttcaa catggcttcg agctttatgt ccctactgtg ccctatagtg tcatggagta 8040ccttgattca cgcccagaca ccccttttat gtgcactaaa cacggcactt ccaaggctgc 8100tgcagaggac ctccaaaaat acgacctatc cacccaagga tttgtcctgc ctggggtcct 8160acgcctagtg cgcaggttca tctttggcca cattggcaag gcaccgccat tgttcctccc 8220atcaacttat cccgccaaga actccatggc aggtattaat ggtcagaggt tcccaacaaa 8280ggatgttcaa agtatacctg aaattgatga aatgtgtgcc cgcgccgtca aggagaattg 8340gcaaactgtg acaccttgca ccctcaagaa acagtattgt tctaggccca aaaccaggac 8400catcctgggc actaacaact tcatagcctt ggctcataga tcggcgctca gtggtgttac 8460ccaggcattc atgaagaagg cttggaagtc cccaatagcc ttagggaaaa acaaattcaa 8520ggagctgcat tgcactgtcg ctggcaggtg cctcgaggcc gacttggctt cctgtgaccg 8580cagcacccct gccattgtga ggtggttcac tacccacctc ctatatgaac ttgcaggatg 8640tgaagaatat ctacctagct atgtgcttaa ctgttgccat gaccttgtgg cgacgcagga 8700tggtgctttc acaaaacgcg gtggcctgtc gtctggagac ccagtcacca gtgtgtccaa 8760cactgtgtac tcactggtga tttatgccca gcacatggta ctatctgccc tgaaaatggg 8820tcatgaaatt ggcctcaagt tcctcgaaga acaactcaaa tttgaggacc ttcttgaaat 8880ccagcctatg ttagtatact ctgatgatct tgtcttgtac gcagaaaagc ccaccttccc 8940caactatcat tggtgggtcg agcatcttga cctgatgttg ggctttaaaa cggacccaaa

9000gaaaaccgtc ataactgata aacccagttt cctcgggtgc agaatcgaag cagggcgaca 9060gctagtcccc aatcgcgacc gcatcctggc tgctcttgca tatcacatga aggcgcagaa 9120cgcctcagag tattatgcgt ctgctgccgc aatcctgatg gattcatgtg cttgcattga 9180ccacgatcct gaatggtatg aggacctcat ctgtggcatt gcccgatgcg ctcgcctgga 9240cggttatagc tttccaggtc cggcattttt catgtccatg tgggagaagc tgaggagtca 9300taatgaaggg aagaaattcc gccactgcgg catctgcgac gccaaagccg actacgcggc 9360tgcttgtggg cttgatttgt gtttgtttca ttcgcacttt caccaacact gccctgtcac 9420tctgagctgc ggtcaccatg ccggttcgaa ggaatgttcg cagtgtcagt cacctgttgg 9480ggccggcaga tcccctcttg atgctgtgct ggaacaaatt ccatacaaac ctcctcgcac 9540tgtcatcatg aaggtgggta ataaaacaac ggcccttgat ccggggaggt accagtcccg 9600tcgaggtctc gttgcagtca agaggggtat tgcgggtaat gaagttgatc ttgctgatgg 9660agattaccaa gtggtacctc ttctgccgac ttgcaaagat ataaacatgg tgaaggtggc 9720ttgcaacgta ctactcagca agttcatagt agggccacca ggttccggga agaccacctg 9780gctactaagt caagttcagg acgatgatgt catttacaca cccactcatc agaccatgtt 9840tgacatagtt agtgctctca aagtttgcag gtattccatt ccaggagcct cgggactccc 9900tttcccaccg cctgccaggt ccgggccgtg ggttaggctc attgccagcg ggcacgtccc 9960tggccgagta tcatacctcg atgaggccgg atattgcaat catctggaca ttcttagact 10020gctttccaaa acaccccttg tgtgtttggg tgaccttcag caacttcacc cagtcggctt 10080tgattcctat tgttatgtgt tcgatcagat gcctcagaag cagttgacca ccatttacag 10140atttggcccc aacatctgcg cagccatcca gccttgttac agggagaaac ttgaatctaa 10200ggctaggaac accagggtgg tttttaccac ccggcctgtg gcctttggcc aggtgctgac 10260accatatcac aaagatcgcg tcggctccgc gattaccata gactcatccc agggggccac 10320ctttgacatt gtaacattgc atctaccatc gccaaagtcc ctaaataagt cccgggcact 10380tgtggccatc acacgggcaa gacacgggtt gttcatttat gaccctcaca accagctccg 10440ggagtttttc aacctaaccc ctgagcgcac tgattgtaac cttgtgttca gccgtggaga 10500tgagctggtg gtcctgaatg cagataatgc agtcacaacc gtggcgaagg ccttagagac 10560aggtccaact caatttcgag tgtcagaccc gaggtgcaag tctctcttag ccgcttgctc 10620ggccagtctg gaagggagct gcatgccgct accgcaagtg gcgcataacc tggggtttta 10680cttctcccca gacagtccag tatttgcacc tctgccaaaa gagttggcgc cacattggcc 10740agtggttacc catcagaata atcgggcgtg gcctgatcga cttgtcgcta gtatgcgtcc 10800aattgacgcc cgctacagca agccgatggt cggtgcaggg tatgtggtcg gaccgtccac 10860cttccttggt actcctggag tggtgtcata ctatctcaca ctatacatca ggggtgagcc 10920ccaggccttg ccagaaacac ttgtttcaac aggacgtata gccacagatt gtcgggagta 10980tctcgacgcg gctgaggaag aggcagcaaa agaacttccc cacgcgttca ttggcgatgt 11040caaaggcacc acagttgggg ggtgtcatca cattacatca aaatacctac ctaggtccct 11100gcctaaagac tctgttgccg tagttggagt aagttcgcct ggcagggctg ctaaagccgt 11160atgcaccctc accgatgtgt acctccctga actccggcca tatctgcaac ctgagacggc 11220atcaaaatgc tggaaactca aattagactt cagggacgtc cgactaatgg tctggaaagg 11280agccaccgcc tactttcaat tggaagggct cacatggtcg gcgctgcctg actatgccag 11340gtttattcag ctgcccaaaa acgctgttgt atacatcgat ccgtgcatag gaccggcaac 11400agccaatcgt aaagtcgtac gaaccacaga ttggcgggcc gacctggcag tgacgccgta 11460tgattacggt gcccggaaca ttttgacaac agcctggttc gaggacctcg ggccgcagtg 11520gaagattctg gggttgcagc cctttaggcg ggcgtttggc tttgaaaaca ctgaggattg 11580ggcaatcctt gcatgctgca tgagtgacgg caaggactac actgactata actggaattg 11640cgttcgacaa cgcccacacg ctatccatgg acgcgctcgt gaccatacgt accactttgc 11700ccctggcact gaattgcaag tggagctcgg taaaccccgg ctgccacctg agcaagtacc 11760gtgaattcgg agtgatgcaa tggggtcact gtggagtaaa atcagccagc tgttcgtgga 11820cgccttcact gaattccttg ttagtgtggt tgatattgtc atcttccttg ccatattgtt 11880tgggttcacc gtcgcaggat ggttactggt ctttcttctc agagtggttt gctccgcgct 11940tctccgttcg cgctctgcca ttcactcttc cgaactatcg aaggtcctat gagggcttac 12000tacctaattg cagaccggat gttccacaat ttgcatttaa gcaccctttg ggtatgtttt 12060ggcacatgcg ggtttcccac ctaattgatc agatggtctc tcgccgcatc taccagacca 12120tggaacattc aggtcaagcg gcctggaagc acgtggtcag tgaggctact cttacaaaat 12180tgtcagaact cgacatagtt ctccacttcc aacacctggc cgcagtggag gcggactctt 12240gtcgcttcct cagctcacga cttgtgatgc tgaaaaatct tgccgttggc aatgtgagct 12300tgcagtacaa caccacgttg aaccgcgttg agctcatcct ccccacacca ggtacgaggc 12360ccaaattgac cgatttcaga caatggctca tcagtgtgca cgcttccatt ttttcctctg 12420tagcctcatc agttactttg ttcatagtgc tttggcttcg aattccagcc gtacgctatg 12480tttttggttt ccattggccc atggcaacac gtcattcgag ctgaccatta attacactat 12540atgcatgccc tgtcttacca gccaagcggc tcaacaaagg ctcgaacccg gtcgtaacat 12600gtggtgcaaa ataggacaca ccacgtgcga ggagcgtgac catgatgagt tgtcaatgtc 12660catcccgtcc gggtacgaca acctcaaact tgaaggttat tacgcttggc tggctttttt 12720gtccttttcc tacgcggccc aattccatcc ggagttgttt ggaataggga atgtgtcgcg 12780cgtctttgtg gataaacgac accagttcat ttgtgccgag catgacggag ataattcaac 12840cgtatctacc ggacacaaca tctccgcatc atatgcggca tattatcacc accaaataga 12900cgggggcaat tggttccatt tggaatggct gcggccgctc ttttcctctt ggctagtgct 12960caacatatca tggtttctga ggcgttcgcc tgcaagccct gtttctcgac gcatctatca 13020gatattaaga ccaatacgac cgcggctgcc ggtttcatgg tccttcaaga catcagttgc 13080ctccgacctc acagggtctc agcatcgcaa gagaacattc ccttcggaaa gtcgtcacaa 13140tgtcgtgaag ccgtcggtac tccccagtac attacgatga ctgctaatgt gaccgacgaa 13200tcatatttgt acaacgcgga cttgctaatg ctttccgcgt gccttttcca cgcctcagaa 13260atgagcgaga aaggcttcaa agttatcttt ggaaacgtct ccggcgttgt ttcagcttgt 13320gtcaatttca cagattatgt ggcccatgta acccaacata cccaacagca tcatctggta 13380attgatcaca ttcggttact gcatttcctg acaccatctg caatgaggtg ggctacaacc 13440attgcttgtt tgttcgccat tctcttagcg atatgagatg ttctcacaaa ttggggcgtt 13500tcttgactcc gcactcttgc ttctggtggc tttttttgct gtgtaccggc ttgtcctggt 13560cctttgccga tggcaacggc aacagctcga cacgccaata catatataac ttgacgatat 13620gcgagctgaa tgggaccgtc tggttgtcca gtcattttga ttgggcagtc gagacctttg 13680tgctttaccc ggtggccact catatcctct cactgggttt tctcacaaca agccattttt 13740ttgatgcgct cggtctcggc gctgtgtcca ctacgggatt tcttggcggg cggtatgtac 13800ttagcagcgt gtacggcgcc tgcgccttcg cagcgcttgt atgttttgtc atccgtgctg 13860ctaaaaattg catggcttgc cgttatgccc gcacccggtt caccaacttc atcgtggacg 13920accgggggaa gatccatcga tggaagtccc caatagtggt agagaaatta ggcaaagctg 13980acatcggcgg cgaccttgtc accatcaaac atgttgtcct cgaaggagtc aaagctcaac 14040ctttgacgag gacatcggcg gagcaatggg aagcctagat gatttttgca atgatcctac 14100cgccgcacag aagcttgtgc tggcatttag tatcacatac acacctataa tgatatacgc 14160cctcaaggtg tcacgcggcc ggctcctagg actgttacac atcctgatat ttctgaactg 14220ttctttcacg ttcggataca tgacatacgt gcactttcaa tccactaacc gtgtcgcgct 14280tactatgggg gcggtcgttg cccttttgtg gggcatttac agctttatag aatcatggaa 14340gtttgtcact tccagatgca ggttgtgttg cctaggccgg cgatacattc tggcccctgc 14400ccaccacgta gaaagtgctg caggcctcca ttcaatccca gcgtctggta accgagcata 14460cgctgtgaga aagcccggac taacatcagt gaacggcact ctagtaccag gacttcggag 14520cctcgtgttg ggcggcaaac gagctgttaa acgaggagtg gttaacctcg tcaagtatgg 14580ccggtaaaaa ccagagccag aagaaaaaga aaaacacagc tcctatgggg agtggccagc 14640cagtcaatca actgtgccaa ttgctgggca caatgataaa gtcccagcgc cagcggccta 14700ggggaggaca ggccaaaatg aaaaagcctg agaagccaca tttcccccta gctgctgaag 14760atgacatccg gcaccatttc acccagaccg agcgttccct ttgcttgcaa tcgatccaga 14820cggccttcaa tcaaggcgca ggaactgcgt cgctttcatc cagcgggaag gtcagttttc 14880aggttgagtt catgctgccg gtcgctcata cggtgcgcct gattcgcgta acttccacat 14940ccgccagtca gggtgcaagt taatttgata gttaggtgaa tggccgcgat tggcgtgtgg 15000cctctgagtc acctattcaa ttagggcgat cacatggggg ttagacttaa ttggcgagaa 15060ccatgtgacc gaaattaaaa aaaaaaaaaa aaaaaaaaaa aaaa 151043415101DNAPorcine reproductive and respiratory syndrome virus 34atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctccc ccatgatgtg gacgccggaa tccgatgatt 840cagccgacct agaggcgcta ccgcctgagc tagaacgtca ggtcgaaatc ctcattcgga 900gttttcctgc ccatcaccct gtcagcctgg ccgactggga gctcgctgag tcccctgaga 960acggtttttc cttcagcacg taccattctg gtggttatct tgtccaaaac cccgacgtgt 1020ttgacagcaa gtgctggctc tcctgtttct tggatcagcc gatcgaagtg cgcctctatg 1080aggattatct ggctaacgct ttcggttacc aaaccaagtg gggcgtgtct ggtaagtacc 1140tccagcgcag gcttcaagtc aacggtattc gtgctgtaat cgatcctgat ggccccattc 1200acgttgaagc gctgtcttgc ccccaatctt ggatcaggca cctgactctg gacgatgacg 1260tcaccccagg attcgttcgc ctaacatccc tccgcattgt gccgaacaca gagcccacta 1320ctctccgaat ctttcggttt ggagcgcata agtggtatgg cgctgccggc aaacgggctc 1380gtgccaagcg tgccgctaaa agtgagaagg gtccggctcc cactcccaag gttgccccgc 1440cagcccccac ctgcggaatt gttacctact ctccaccaac agacgggtct tgcggttggc 1500acgtccttgc cgccataatg aaccgaatga tgaacggtga cttcatgtcc cctctggccc 1560agtacaacag accagaggat gattgggctt ctgattacga tcttgcccag gcgattcaat 1620gtctgcggtt gcctgctacc atagttcgga atcgtgcctg tcccaacgcc aagtacctca 1680taaaactcaa cggagtccac tgggaggtag aggtgaggtc agggatggcc cctcgctccc 1740ttccccgcga gtgcgtagtc ggcgtttgtt ccgaaggctg tgccgcatcg ccttacccag 1800aaaacgggct acctaaacga gcgtttgagg ccttggcgtc tgcttacaga ctaccttccg 1860attgtgtcag ttctggtatt gctgactttc ttgctaaccc ccctcaggaa ttctggactc 1920ttgacaaaat gttgacctcc ccgtcaccgg agcggtctgg tttttccagt ctgtacaaat 1980tgctattaga ggttgtcccg cagaaatgcg gagccacgga aggggctttc acctatgctg 2040ttgagagaat gctgaaggat tgcccgagct ccgaacaggc catggccctt ctggcaaaaa 2100ttaaagttcc atcctcaaag gccccgtctg tgtccctgga cgagtgtttc cctacggatg 2160tttcagccga tttcgaacca gcatctcagg agaggtccca aaattccagc gctgctgttg 2220tcctgcattc accgaatgca aaagagttcg aggaagcagc tccaggggaa gttcaggagg 2280gtggccacaa ggccgtccac tctgcactcc ctgccggggg tcctaacaat aagcaggcac 2340agctggttgc cggtgagcaa ctgaagctcg gcggttgtgg ttcggtagtt gggaatgcac 2400atgaaggtgt tctggtccca cctggtccaa ttaatttgac aagcggggat ttaccctcct 2460caggctccat gaaagaagat atgctcaata gccgggagga cgaaccactg gatttgtccc 2520aaccagcaac agctgtcaca acgactctta tgggagagct aacacccgac tacctaggtt 2580ctgatactgg tgccctcccc gtcaccgtcc gaaaatttgt cccgacgggg cctatactcc 2640gtcatgttga gcactgcagc acggggtcgg gcgatagcag ttcgcctttg gatctgtctg 2700gtgcgcaaac cccggaccag cctttaaatc tgtccctggc ggcttggcca gtgaggacca 2760ccgcgtctga tcctggctgg gtccacggta gacgcgagcc tgtctttgta aagcctcgag 2820atgttttctc tgatggcgat tcagcccttc agttcgggga gctttctgaa tccagctctg 2880tcatcgagtt tgatcgggca aaagatgctc aggtggctga cgcccctgtc ggtctgacga 2940cttcggacga ggccctctcc gcagtcgatc ctttcgagtt ttccgaactc aagcgcccac 3000gtttctccgc acaagcctta attgaccgag gcggcccact tgccgatgtc catgcaaaaa 3060taaagaaccg ggtatatgaa cagtgcctcc aagcttgtga gcccggtagt cgcgcaaccc 3120cagctaccag ggagtggctc gacaaaatgt gggagagagt ggacatgaaa acctggcgct 3180gcacctcaca gttccaagct ggtcacattc ttgcgtccct caaattcctc cctgacatga 3240ttcaagacac accgcctcct gttcccagga agaaccgagc cagtgataaa gctggcctga 3300aacaactagt ggcacagtgg gataggagat tgagttcaac ccccccccca aaaccggttg 3360ggccggtact tgaccgggtc gatcctccgc ctacgggtac ccggcaagaa gacgtcaccc 3420cctccgatgg gccaccccat gcgccggatg gtcgagtgag tacgggtggg agttggaaag 3480gccttatgct ttccggcacc cgtctcacgg ggtccatcag tcatcgcctc atgacatggg 3540tttttgaagt tgtctcccat ctcccagctt ttatgctcac acttttctcg ccgcggggct 3600ctatggctcc aggcgattgg gtttttgcag gtgttgtttt acttgctctc ctgctctgtc 3660gttcttaccc aatattcggg tgccttccct tattgggtgt cttttctggt actgtgcggc 3720gtgttcgtct gggtgttttt ggctcttgga tggcttttgc tgtattttta ttctcgactc 3780catccaaccc agtcggttct tcttgtaacc acgattcgcc ggagtgtcat gctgagcttc 3840tggctcttga gcagcgccaa ctttgggaac ctgtgcgcgg ccttgtggtc ggcccatcgg 3900gcctcttatg tgtcattctt ggcaagttac tcggtgggtc acgttatctc tggcatgttc 3960tcctacgtct atgcctgctt gcagatttgg ccctttctct tgtttatgtg gtgtcccagg 4020ggcgttgtca caagtgttgg ggaaagtgta taaggacagc tcctgctgag gtggctttta 4080atgtatttcc tttctcgcgc gccacccgta gttctcttgt atccttatgt gatcgattcc 4140aaacgccaaa aggagttgat cccgtgcact tggcgacagg ttggcgcggg tgctggcgtg 4200gtgagagtcc tatccatcaa ccacaccaaa agcccatagc ttacgccaat ttggatgaaa 4260agaagatatc tgcccaaacg gttgttgctg tcccatacga tcccaatcag gctatcaaat 4320gtctgaaagt tctgcaggcg ggaggggcta tcgtggatca gcctacgcct gaggtcgttc 4380gtgtgtccga gatccccttc tcagccccat ttttcccaaa agttccagtc aacccagatt 4440gtagggtcgt ggtggattcg gacacttttg tggctgcggt tcgttgtggt tattcgacat 4500cacaactggt cctgggccag ggcaactttg ccaagttaaa tcaaaccccc cccaggaact 4560ctatctccac caaagcgact ggtggggcct cttatacttt tgctgtggct caagtgtctg 4620tgtggaccct tgttcatttc gtcctcggtc tttggctcac gtcacctcaa gtgtgtggtc 4680gaggaaccgc tgacccatgg tgttcaagtc cattttcata tcctacctat ggccccgggg 4740ttgtgtgctc ctctcgactt tgtgtgtctg ctgacggggt cacccttcca ttgttctcag 4800ccgtggcaca actctctggt agggaggtgg ggatttttat tttagtgctc gtctccttta 4860ttgccttggc ccaccgcatg gctcttaagg cagacatgtt agtagtcttt ttggctcttt 4920gtgcttatgc ctggcccatg agctcctggt tgatctgctt ctttcctata ctcttgaggt 4980gggttaccct tcaccctctc actatgcttt gggtgcattc attcttgatg ttttgtctcc 5040cagcagccgg cgtcctctca ctggggataa ctggcctcct ctgggcaatc ggccgcttta 5100ctcaggttgc cggaatcatt acaccttatg atatccacca gtacacctct gggccacgtg 5160gtgcagccgc tgtggccaca gccccagaag gcacttacat ggccgccgtc cggagagctg 5220ctttaaccgg gcggacttta atcttcaccc cgtccgcagt tggatccctt ctcgaaggtg 5280ctttcaggac tcataaaccc tgccttaaca ccgtgaatgt cgtaggctcc tcccttggtt 5340ccggaggggt tttcaccatt gacggaaaaa aaattgtcgt cactgctgcc catgtgctga 5400acggcgacac agctagagtc accggtgatt cctacaaccg catgcacact ttcaagacta 5460atggtgacta tgcctggtcc catgctgata actggcaggg cgctgcccct gtggtcaagg 5520ttgcgaaagg gtatcgcggt cgtgcctact ggcaaacatc aactggtgtc gagcctggtg 5580ttattgggaa tgggttcgcc ttctgtttca ccaactgcgg cgattcgggg tcacccgtta 5640tctcagaatc tggtgatctt atcggaatcc acaccggttc aaacaaactt ggttctggtc 5700ttgtgacaac ccctgaaggg gagacctgta ccatcagaga aaccaagctt tctgaccttt 5760ccagacattt cgcaggccca agcgttcctc ttggggacat caaattgagt ccggccatca 5820tccctgatgt gacatccatt ccgagtgact tggcatcgct cctagcttcc gtccctgtaa 5880tggaaggcgg cctctcgacc gttcaacttt tgtgtgtctt tttcctcctc tggcgcatga 5940tgggccatgc ctggacgccc attgttgccg tgggcttctt tttgctgaat gaaattcttc 6000cagcagtttt ggttcgagcc gtgttttctt ttgcactctt tgtgcttgca tgggccaccc 6060cctggtctgc acaagtgttg atgattagac ttctcacggc atctctcaac cgcaacaaac 6120tttctcttgc gttctacgca ctcggaggtg ttgttggttt ggctgctgaa atcgggactt 6180ttgctggtaa attgtctgaa ttgtctcaag ctctttcgac atactgtttc ttacctaggg 6240tccttgctat gaccagctgt gttcccatca tcatcattgg tggactccat gccctcggtg 6300tgattctgtg gttattcaaa taccggtgcc tccacaacat gctggttggt gatggaagct 6360tttcaagcgc tttcttccta cggtattttg cagagggcaa tctcaggaga ggtgtttcac 6420agtcctgtgg catgagtaac gagtccctga cggctgcttt ggcttgcaag ttgtcacagg 6480ctgaccttga ttttttgtcc agcttaacga acttcaagtg ctttgtatct gcttcaaaca 6540tgaaaaatgc tgccggccag tacattgaag cagcttatgc cagggccctg cgtcaagagt 6600tggcctcttt agtccagatt gacaaaatga aaggagtttt gtccaagcta gaggcctttg 6660ctgaaacggc cactccgtcc ctcgacgtag gtgacgtgat tgttctactt ggacaacatc 6720ctcacggatc cgttctcgat attaatgtgg ggactgaaag gaaaactgta tccgtgcaag 6780agacccggag cctaggcggc tccaagttca gtgtttgtac tgttgtgtca aacacacccg 6840tggacgcctt agccggtatt ccactccaga caccaacccc ccttttcgag aatggcccgc 6900gtcatcgcag cgaggaggac gatcttaaag tcgagaggat gaagaaacac tgcgtgtccc 6960tcggcttcca caacattaac ggtaaagttt actgcaagat ttgggacaag tctaccggtg 7020acgcctttta cactgatgat tcccggtaca cccaagacta tgcttttcag gacaggtcag 7080ctgactatag agacagggac tacgagggtg tgcaaaccgc cccccaacag ggatttgatc 7140caaagtctga aacccctgtt ggtaccgttg tgatcggcgg tattacgtac aacaggtatt 7200tggtcaaagg taaggaggtt ctggttccca agcctgacaa ctgccttgaa gctgccaagc 7260tgtcccttga gcaagctctc gctgggatgg gccaaacttg tgaccttaca gctgccgagg 7320tggaaaagct aaagcgcatc attggtcaac ttcaaggatt gaccactgag caggctttaa 7380actgttagcc gccagcggct tgacccgctg tggccgcggc ggcctagttg taactgaaac 7440ggcggtaaaa attgtcaaat accacagcag aacttttacc ttaggctctt tagacctaaa 7500agtcacttcc gaggtggagg tgaagaagtc aaccgagcag ggccacgctg ttgtggcaaa 7560cttgtgttct ggtgtcgtct tgatgagacc tcacccaccg tcccttgtcg acgttcttct 7620gaaacccgga cttgacataa cacccggcat tcaaccaggg catggggccg ggaatatggg 7680cgtggacggt tccatttggg attttgaaac cgcacccaca aaggctgaac tcgagttatc 7740caagcaaata attcaagcat gtgaagtcag gcgcggggat gccccgaacc tccagctccc 7800ttacaagctc tatcctgtta gaggggatcc tgagcggcat aaaggccacc tcatcaatac 7860caggtttgga gacttacctt acaaaactcc tcaagacacc aagtccgcaa tccacgcggc 7920ttgttgcctg caccccaacg gggcccccgt gtctgatggt aaatccacac taggtaccac 7980tcttcaacat ggcttcgagc tttatgtccc tactgtgccc tatagtgtca tggagtacct 8040tgattcacgc ccagacaccc cttttatgtg cactaaacac ggcacttcca aggctgctgc 8100agaggacctc caaaaatacg acctatccac ccaaggattt gtcctgcctg gggtcctacg 8160cctagtgcgc aggttcatct ttggccacat tggcaaggca ccgccattgt tcctcccatc 8220aacttatccc gccaagaact ccatggcagg tattaatggt cagaggttcc caacaaagga 8280tgttcaaagt atacctgaaa ttgatgaaat gtgtgcccgc gccgtcaagg agaattggca 8340aactgtgaca ccttgcaccc tcaagaaaca gtattgttct aggcccaaaa ccaggaccat 8400cctgggcact aacaacttca tagccttggc tcatagatcg gcgctcagtg gtgttaccca 8460ggcattcatg aagaaggctt ggaagtcccc aatagcctta gggaaaaaca aattcaagga 8520gctgcattgc actgtcgctg gcaggtgcct cgaggccgac ttggcttcct gtgaccgcag 8580cacccctgcc attgtgaggt ggttcactac ccacctccta tatgaacttg caggatgtga 8640agaatatcta cctagctatg tgcttaactg ttgccatgac cttgtggcga cgcaggatgg 8700tgctttcaca aaacgcggtg gcctgtcgtc tggagaccca gtcaccagtg tgtccaacac 8760tgtgtactca ctggtgattt atgcccagca catggtacta tctgccctga aaatgggtca 8820tgaaattggc ctcaagttcc tcgaagaaca actcaaattt gaggaccttc ttgaaatcca

8880gcctatgtta gtatactctg atgatcttgt cttgtacgca gaaaagccca ccttccccaa 8940ctatcattgg tgggtcgagc atcttgacct gatgttgggc tttaaaacgg acccaaagaa 9000aaccgtcata actgataaac ccagtttcct cgggtgcaga atcgaagcag ggcgacagct 9060agtccccaat cgcgaccgca tcctggctgc tcttgcatat cacatgaagg cgcagaacgc 9120ctcagagtat tatgcgtctg ctgccgcaat cctgatggat tcatgtgctt gcattgacca 9180cgatcctgaa tggtatgagg acctcatctg tggcattgcc cgatgcgctc gcctggacgg 9240ttatagcttt ccaggtccgg catttttcat gtccatgtgg gagaagctga ggagtcataa 9300tgaagggaag aaattccgcc actgcggcat ctgcgacgcc aaagccgact acgcggctgc 9360ttgtgggctt gatttgtgtt tgtttcattc gcactttcac caacactgcc ctgtcactct 9420gagctgcggt caccatgccg gttcgaagga atgttcgcag tgtcagtcac ctgttggggc 9480cggcagatcc cctcttgatg ctgtgctgga acaaattcca tacaaacctc ctcgcactgt 9540catcatgaag gtgggtaata aaacaacggc ccttgatccg gggaggtacc agtcccgtcg 9600aggtctcgtt gcagtcaaga ggggtattgc gggtaatgaa gttgatcttg ctgatggaga 9660ttaccaagtg gtacctcttc tgccgacttg caaagatata aacatggtga aggtggcttg 9720caacgtacta ctcagcaagt tcatagtagg gccaccaggt tccgggaaga ccacctggct 9780actaagtcaa gttcaggacg atgatgtcat ttacacaccc actcatcaga ccatgtttga 9840catagttagt gctctcaaag tttgcaggta ttccattcca ggagcctcgg gactcccttt 9900cccaccgcct gccaggtccg ggccgtgggt taggctcatt gccagcgggc acgtccctgg 9960ccgagtatca tacctcgatg aggccggata ttgcaatcat ctggacattc ttagactgct 10020ttccaaaaca ccccttgtgt gtttgggtga ccttcagcaa cttcacccag tcggctttga 10080ttcctattgt tatgtgttcg atcagatgcc tcagaagcag ttgaccacca tttacagatt 10140tggccccaac atctgcgcag ccatccagcc ttgttacagg gagaaacttg aatctaaggc 10200taggaacacc agggtggttt ttaccacccg gcctgtggcc tttggccagg tgctgacacc 10260atatcacaaa gatcgcgtcg gctccgcgat taccatagac tcatcccagg gggccacctt 10320tgacattgta acattgcatc taccatcgcc aaagtcccta aataagtccc gggcacttgt 10380ggccatcaca cgggcaagac acgggttgtt catttatgac cctcacaacc agctccggga 10440gtttttcaac ctaacccctg agcgcactga ttgtaacctt gtgttcagcc gtggagatga 10500gctggtggtc ctgaatgcag ataatgcagt cacaaccgtg gcgaaggcct tagagacagg 10560tccaactcaa tttcgagtgt cagacccgag gtgcaagtct ctcttagccg cttgctcggc 10620cagtctggaa gggagctgca tgccgctacc gcaagtggcg cataacctgg ggttttactt 10680ctccccagac agtccagtat ttgcacctct gccaaaagag ttggcgccac attggccagt 10740ggttacccat cagaataatc gggcgtggcc tgatcgactt gtcgctagta tgcgtccaat 10800tgacgcccgc tacagcaagc cgatggtcgg tgcagggtat gtggtcggac cgtccacctt 10860ccttggtact cctggagtgg tgtcatacta tctcacacta tacatcaggg gtgagcccca 10920ggccttgcca gaaacacttg tttcaacagg acgtatagcc acagattgtc gggagtatct 10980cgacgcggct gaggaagagg cagcaaaaga acttccccac gcgttcattg gcgatgtcaa 11040aggcaccaca gttggggggt gtcatcacat tacatcaaaa tacctaccta ggtccctgcc 11100taaagactct gttgccgtag ttggagtaag ttcgcctggc agggctgcta aagccgtatg 11160caccctcacc gatgtgtacc tccctgaact ccggccatat ctgcaacctg agacggcatc 11220aaaatgctgg aaactcaaat tagacttcag ggacgtccga ctaatggtct ggaaaggagc 11280caccgcctac tttcaattgg aagggctcac atggtcggcg ctgcctgact atgccaggtt 11340tattcagctg cccaaaaacg ctgttgtata catcgatccg tgcataggac cggcaacagc 11400caatcgtaaa gtcgtacgaa ccacagattg gcgggccgac ctggcagtga cgccgtatga 11460ttacggtgcc cggaacattt tgacaacagc ctggttcgag gacctcgggc cgcagtggaa 11520gattctgggg ttgcagccct ttaggcgggc gtttggcttt gaaaacactg aggattgggc 11580aatccttgca tgctgcatga gtgacggcaa ggactacact gactataact ggaattgcgt 11640tcgacaacgc ccacacgcta tccatggacg cgctcgtgac catacgtacc actttgcccc 11700tggcactgaa ttgcaagtgg agctcggtaa accccggctg ccacctgagc aagtaccgtg 11760aattcggagt gatgcaatgg ggtcactgtg gagtaaaatc agccagctgt tcgtggacgc 11820cttcactgaa ttccttgtta gtgtggttga tattgtcatc ttccttgcca tattgtttgg 11880gttcaccgtc gcaggatggt tactggtctt tcttctcaga gtggtttgct ccgcgcttct 11940ccgttcgcgc tctgccattc actcttccga actatcgaag gtcctatgag ggcttactac 12000ctaattgcag accggatgtt ccacaatttg catttaagca ccctttgggt atgttttggc 12060acatgcgggt ttcccaccta attgatcaga tggtctctcg ccgcatctac cagaccatgg 12120aacattcagg tcaagcggcc tggaagcacg tggtcagtga ggctactctt acaaaattgt 12180cagaactcga catagttctc cacttccaac acctggccgc agtggaggcg gactcttgtc 12240gcttcctcag ctcacgactt gtgatgctga aaaatcttgc cgttggcaat gtgagcttgc 12300agtacaacac cacgttgaac cgcgttgagc tcatcctccc cacaccaggt acgaggccca 12360aattgaccga tttcagacaa tggctcatca gtgtgcacgc ttccattttt tcctctgtag 12420cctcatcagt tactttgttc atagtgcttt ggcttcgaat tccagccgta cgctatgttt 12480ttggtttcca ttggcccatg gcaacacgtc attcgagctg accattaatt acactatatg 12540catgccctgt cttaccagcc aagcggctca acaaaggctc gaacccggtc gtaacatgtg 12600gtgcaaaata ggacacacca cgtgcgagga gcgtgaccat gatgagttgt caatgtccat 12660cccgtccggg tacgacaacc tcaaacttga aggttattac gcttggctgg cttttttgtc 12720cttttcctac gcggcccaat tccatccgga gttgtttgga atagggaatg tgtcgcgcgt 12780ctttgtggat aaacgacacc agttcatttg tgccgagcat gacggagata attcaaccgt 12840atctaccgga cacaacatct ccgcatcata tgcggcatat tatcaccacc aaatagacgg 12900gggcaattgg ttccatttgg aatggctgcg gccgctcttt tcctcttggc tagtgctcaa 12960catatcatgg tttctgaggc gttcgcctgc aagccctgtt tctcgacgca tctatcagat 13020attaagacca atacgaccgc ggctgccggt ttcatggtcc ttcaagacat cagttgcctc 13080cgacctcaca gggtctcagc atcgcaagag aacattccct tcggaaagtc gtcacaatgt 13140cgtgaagccg tcggtactcc ccagtacatt acgatgactg ctaatgtgac cgacgaatca 13200tatttgtaca acgcggactt gctaatgctt tccgcgtgcc ttttccacgc ctcagaaatg 13260agcgagaaag gcttcaaagt tatctttgga aacgtctccg gcgttgtttc agcttgtgtc 13320aatttcacag attatgtggc ccatgtaacc caacataccc aacagcatca tctggtaatt 13380gatcacattc ggttactgca tttcctgaca ccatctgcaa tgaggtgggc tacaaccatt 13440gcttgtttgt tcgccattct cttagcgata tgagatgttc tcacaaattg gggcgtttct 13500tgactccgca ctcttgcttc tggtggcttt ttttgctgtg taccggcttg tcctggtcct 13560ttgccgatgg caacggcaac agctcgacac gccaatacat atataacttg acgatatgcg 13620agctgaatgg gaccgtctgg ttgtccagtc attttgattg ggcagtcgag acctttgtgc 13680tttacccggt ggccactcat atcctctcac tgggttttct cacaacaagc catttttttg 13740atgcgctcgg tctcggcgct gtgtccacta cgggatttct tggcgggcgg tatgtactta 13800gcagcgtgta cggcgcctgc gccttcgcag cgcttgtatg ttttgtcatc cgtgctgcta 13860aaaattgcat ggcttgccgt tatgcccgca cccggttcac caacttcatc gtggacgacc 13920gggggaagat ccatcgatgg aagtccccaa tagtggtaga gaaattaggc aaagctgaca 13980tcggcggcga ccttgtcacc atcaaacatg ttgtcctcga aggagtcaaa gctcaacctt 14040tgacgaggac atcggcggag caatgggaag cctagatgat ttttgcaatg atcctaccgc 14100cgcacagaag cttgtgctgg catttagtat cacatacaca cctataatga tatacgccct 14160caaggtgtca cgcggccggc tcctaggact gttacacatc ctgatatttc tgaactgttc 14220tttcacgttc ggatacatga catacgtgca ctttcaatcc actaaccgtg tcgcgcttac 14280tatgggggcg gtcgttgccc ttttgtgggg catttacagc tttatagaat catggaagtt 14340tgtcacttcc agatgcaggt tgtgttgcct aggccggcga tacattctgg cccctgccca 14400ccacgtagaa agtgctgcag gcctccattc aatcccagcg tctggtaacc gagcatacgc 14460tgtgagaaag cccggactaa catcagtgaa cggcactcta gtaccaggac ttcggagcct 14520cgtgttgggc ggcaaacgag ctgttaaacg aggagtggtt aacctcgtca agtatggccg 14580gtaaaaacca gagccagaag aaaaagaaaa acacagctcc tatggggagt ggccagccag 14640tcaatcaact gtgccaattg ctgggcacaa tgataaagtc ccagcgccag cggcctaggg 14700gaggacaggc caaaatgaaa aagcctgaga agccacattt ccccctagct gctgaagatg 14760acatccggca ccatttcacc cagaccgagc gttccctttg cttgcaatcg atccagacgg 14820ccttcaatca aggcgcagga actgcgtcgc tttcatccag cgggaaggtc agttttcagg 14880ttgagttcat gctgccggtc gctcatacgg tgcgcctgat tcgcgtaact tccacatccg 14940ccagtcaggg tgcaagttaa tttgatagtt aggtgaatgg ccgcgattgg cgtgtggcct 15000ctgagtcacc tattcaatta gggcgatcac atgggggtta gacttaattg gcgagaacca 15060tgtgaccgaa attaaaaaaa aaaaaaaaaa aaaaaaaaaa a 151013515098DNAPorcine reproductive and respiratory syndrome virus 35atgatgtgta gggtattccc cctacataca cgacactact agtgtttgtg taccttggag 60gcgtgggtac agccccgccc caccccttgg cccctgttct agcccaacag gtatccttct 120ctctcggggc gagtgtgccg cctgctgctc ccttgcagcg ggaaggacct cccgagtatt 180cccggagagc acctgcttta cgggatctcc accctttaac catgtctggg acgttctccc 240ggtgcatgtg caccccggct gctcgggtat tttggaacgc cggccaagtc tattgcacac 300ggtgtctcag tgcgcggtct cttctccctc cggaacttca ggacattgac ctcgccgcaa 360ttggcttgtt ttacaagcct aaagacaagc ttcactggaa agtccctatc ggcatccctc 420aggtggagtg tactccatcc gggtgttgtt ggctctcagg cattttcccc ttagcgcgca 480tgacctccgg caatcacaac ttcctacaac gacttgtgaa agttgccgat gtgttgtacc 540gtgatggttg cttaacttct cgacaccttc gtgaactcca agtttacgag cgtggctgca 600gctggtaccc aatcacgggg ccagtgcccg ggatgggttt gtacgcaaat tccatgcacg 660tatctgacca gccgttccct ggtgccaccc atgtgttgac gaactcgcct ttgcctcaac 720aagcttgtcg gcagccgttc tgcccatttg aggaggctca ttctagcgtg tacaggtgga 780aaaaatttgt ggtcttcacg gactcctcca tgatgtggac gccggaatcc gatgattcag 840ccgacctaga ggcgctaccg cctgagctag aacgtcaggt cgaaatcctc attcggagtt 900ttcctgccca tcaccctgtc agcctggccg actgggagct cgctgagtcc cctgagaacg 960gtttttcctt cagcacgtac cattctggtg gttatcttgt ccaaaacccc gacgtgtttg 1020acagcaagtg ctggctctcc tgtttcttgg atcagccgat cgaagtgcgc ctctatgagg 1080attatctggc taacgctttc ggttaccaaa ccaagtgggg cgtgtctggt aagtacctcc 1140agcgcaggct tcaagtcaac ggtattcgtg ctgtaatcga tcctgatggc cccattcacg 1200ttgaagcgct gtcttgcccc caatcttgga tcaggcacct gactctggac gatgacgtca 1260ccccaggatt cgttcgccta acatccctcc gcattgtgcc gaacacagag cccactactc 1320tccgaatctt tcggtttgga gcgcataagt ggtatggcgc tgccggcaaa cgggctcgtg 1380ccaagcgtgc cgctaaaagt gagaagggtc cggctcccac tcccaaggtt gccccgccag 1440cccccacctg cggaattgtt acctactctc caccaacaga cgggtcttgc ggttggcacg 1500tccttgccgc cataatgaac cgaatgatga acggtgactt catgtcccct ctggcccagt 1560acaacagacc agaggatgat tgggcttctg attacgatct tgcccaggcg attcaatgtc 1620tgcggttgcc tgctaccata gttcggaatc gtgcctgtcc caacgccaag tacctcataa 1680aactcaacgg agtccactgg gaggtagagg tgaggtcagg gatggcccct cgctcccttc 1740cccgcgagtg cgtagtcggc gtttgttccg aaggctgtgc cgcatcgcct tacccagaaa 1800acgggctacc taaacgagcg tttgaggcct tggcgtctgc ttacagacta ccttccgatt 1860gtgtcagttc tggtattgct gactttcttg ctaacccccc tcaggaattc tggactcttg 1920acaaaatgtt gacctccccg tcaccggagc ggtctggttt ttccagtctg tacaaattgc 1980tattagaggt tgtcccgcag aaatgcggag ccacggaagg ggctttcacc tatgctgttg 2040agagaatgct gaaggattgc ccgagctccg aacaggccat ggcccttctg gcaaaaatta 2100aagttccatc ctcaaaggcc ccgtctgtgt ccctggacga gtgtttccct acggatgttt 2160cagccgattt cgaaccagca tctcaggaga ggtcccaaaa ttccagcgct gctgttgtcc 2220tgcattcacc gaatgcaaaa gagttcgagg aagcagctcc aggggaagtt caggagggtg 2280gccacaaggc cgtccactct gcactccctg ccgggggtcc taacaataag caggcacagc 2340tggttgccgg tgagcaactg aagctcggcg gttgtggttc ggtagttggg aatgcacatg 2400aaggtgttct ggtcccacct ggtccaatta atttgacaag cggggattta ccctcctcag 2460gctccatgaa agaagatatg ctcaatagcc gggaggacga accactggat ttgtcccaac 2520cagcaacagc tgtcacaacg actcttatgg gagagctaac acccgactac ctaggttctg 2580atactggtgc cctccccgtc accgtccgaa aatttgtccc gacggggcct atactccgtc 2640atgttgagca ctgcagcacg gggtcgggcg atagcagttc gcctttggat ctgtctggtg 2700cgcaaacccc ggaccagcct ttaaatctgt ccctggcggc ttggccagtg aggaccaccg 2760cgtctgatcc tggctgggtc cacggtagac gcgagcctgt ctttgtaaag cctcgagatg 2820ttttctctga tggcgattca gcccttcagt tcggggagct ttctgaatcc agctctgtca 2880tcgagtttga tcgggcaaaa gatgctcagg tggctgacgc ccctgtcggt ctgacgactt 2940cggacgaggc cctctccgca gtcgatcctt tcgagttttc cgaactcaag cgcccacgtt 3000tctccgcaca agccttaatt gaccgaggcg gcccacttgc cgatgtccat gcaaaaataa 3060agaaccgggt atatgaacag tgcctccaag cttgtgagcc cggtagtcgc gcaaccccag 3120ctaccaggga gtggctcgac aaaatgtggg agagagtgga catgaaaacc tggcgctgca 3180cctcacagtt ccaagctggt cacattcttg cgtccctcaa attcctccct gacatgattc 3240aagacacacc gcctcctgtt cccaggaaga accgagccag tgataaagct ggcctgaaac 3300aactagtggc acagtgggat aggagattga gttcaacccc ccccccaaaa ccggttgggc 3360cggtacttga ccgggtcgat cctccgccta cgggtacccg gcaagaagac gtcaccccct 3420ccgatgggcc accccatgcg ccggatggtc gagtgagtac gggtgggagt tggaaaggcc 3480ttatgctttc cggcacccgt ctcacggggt ccatcagtca tcgcctcatg acatgggttt 3540ttgaagttgt ctcccatctc ccagctttta tgctcacact tttctcgccg cggggctcta 3600tggctccagg cgattgggtt tttgcaggtg ttgttttact tgctctcctg ctctgtcgtt 3660cttacccaat attcgggtgc cttcccttat tgggtgtctt ttctggtact gtgcggcgtg 3720ttcgtctggg tgtttttggc tcttggatgg cttttgctgt atttttattc tcgactccat 3780ccaacccagt cggttcttct tgtaaccacg attcgccgga gtgtcatgct gagcttctgg 3840ctcttgagca gcgccaactt tgggaacctg tgcgcggcct tgtggtcggc ccatcgggcc 3900tcttatgtgt cattcttggc aagttactcg gtgggtcacg ttatctctgg catgttctcc 3960tacgtctatg cctgcttgca gatttggccc tttctcttgt ttatgtggtg tcccaggggc 4020gttgtcacaa gtgttgggga aagtgtataa ggacagctcc tgctgaggtg gcttttaatg 4080tatttccttt ctcgcgcgcc acccgtagtt ctcttgtatc cttatgtgat cgattccaaa 4140cgccaaaagg agttgatccc gtgcacttgg cgacaggttg gcgcgggtgc tggcgtggtg 4200agagtcctat ccatcaacca caccaaaagc ccatagctta cgccaatttg gatgaaaaga 4260agatatctgc ccaaacggtt gttgctgtcc catacgatcc caatcaggct atcaaatgtc 4320tgaaagttct gcaggcggga ggggctatcg tggatcagcc tacgcctgag gtcgttcgtg 4380tgtccgagat ccccttctca gccccatttt tcccaaaagt tccagtcaac ccagattgta 4440gggtcgtggt ggattcggac acttttgtgg ctgcggttcg ttgtggttat tcgacatcac 4500aactggtcct gggccagggc aactttgcca agttaaatca aacccccccc aggaactcta 4560tctccaccaa agcgactggt ggggcctctt atacttttgc tgtggctcaa gtgtctgtgt 4620ggacccttgt tcatttcgtc ctcggtcttt ggctcacgtc acctcaagtg tgtggtcgag 4680gaaccgctga cccatggtgt tcaagtccat tttcatatcc tacctatggc cccggggttg 4740tgtgctcctc tcgactttgt gtgtctgctg acggggtcac ccttccattg ttctcagccg 4800tggcacaact ctctggtagg gaggtgggga tttttatttt agtgctcgtc tcctttattg 4860ccttggccca ccgcatggct cttaaggcag acatgttagt agtctttttg gctctttgtg 4920cttatgcctg gcccatgagc tcctggttga tctgcttctt tcctatactc ttgaggtggg 4980ttacccttca ccctctcact atgctttggg tgcattcatt cttgatgttt tgtctcccag 5040cagccggcgt cctctcactg gggataactg gcctcctctg ggcaatcggc cgctttactc 5100aggttgccgg aatcattaca ccttatgata tccaccagta cacctctggg ccacgtggtg 5160cagccgctgt ggccacagcc ccagaaggca cttacatggc cgccgtccgg agagctgctt 5220taaccgggcg gactttaatc ttcaccccgt ccgcagttgg atcccttctc gaaggtgctt 5280tcaggactca taaaccctgc cttaacaccg tgaatgtcgt aggctcctcc cttggttccg 5340gaggggtttt caccattgac ggaaaaaaaa ttgtcgtcac tgctgcccat gtgctgaacg 5400gcgacacagc tagagtcacc ggtgattcct acaaccgcat gcacactttc aagactaatg 5460gtgactatgc ctggtcccat gctgataact ggcagggcgc tgcccctgtg gtcaaggttg 5520cgaaagggta tcgcggtcgt gcctactggc aaacatcaac tggtgtcgag cctggtgtta 5580ttgggaatgg gttcgccttc tgtttcacca actgcggcga ttcggggtca cccgttatct 5640cagaatctgg tgatcttatc ggaatccaca ccggttcaaa caaacttggt tctggtcttg 5700tgacaacccc tgaaggggag acctgtacca tcagagaaac caagctttct gacctttcca 5760gacatttcgc aggcccaagc gttcctcttg gggacatcaa attgagtccg gccatcatcc 5820ctgatgtgac atccattccg agtgacttgg catcgctcct agcttccgtc cctgtaatgg 5880aaggcggcct ctcgaccgtt caacttttgt gtgtcttttt cctcctctgg cgcatgatgg 5940gccatgcctg gacgcccatt gttgccgtgg gcttcttttt gctgaatgaa attcttccag 6000cagttttggt tcgagccgtg ttttcttttg cactctttgt gcttgcatgg gccaccccct 6060ggtctgcaca agtgttgatg attagacttc tcacggcatc tctcaaccgc aacaaacttt 6120ctcttgcgtt ctacgcactc ggaggtgttg ttggtttggc tgctgaaatc gggacttttg 6180ctggtaaatt gtctgaattg tctcaagctc tttcgacata ctgtttctta cctagggtcc 6240ttgctatgac cagctgtgtt cccatcatca tcattggtgg actccatgcc ctcggtgtga 6300ttctgtggtt attcaaatac cggtgcctcc acaacatgct ggttggtgat ggaagctttt 6360caagcgcttt cttcctacgg tattttgcag agggcaatct caggagaggt gtttcacagt 6420cctgtggcat gagtaacgag tccctgacgg ctgctttggc ttgcaagttg tcacaggctg 6480accttgattt tttgtccagc ttaacgaact tcaagtgctt tgtatctgct tcaaacatga 6540aaaatgctgc cggccagtac attgaagcag cttatgccag ggccctgcgt caagagttgg 6600cctctttagt ccagattgac aaaatgaaag gagttttgtc caagctagag gcctttgctg 6660aaacggccac tccgtccctc gacgtaggtg acgtgattgt tctacttgga caacatcctc 6720acggatccgt tctcgatatt aatgtgggga ctgaaaggaa aactgtatcc gtgcaagaga 6780cccggagcct aggcggctcc aagttcagtg tttgtactgt tgtgtcaaac acacccgtgg 6840acgccttagc cggtattcca ctccagacac caacccccct tttcgagaat ggcccgcgtc 6900atcgcagcga ggaggacgat cttaaagtcg agaggatgaa gaaacactgc gtgtccctcg 6960gcttccacaa cattaacggt aaagtttact gcaagatttg ggacaagtct accggtgacg 7020ccttttacac tgatgattcc cggtacaccc aagactatgc ttttcaggac aggtcagctg 7080actatagaga cagggactac gagggtgtgc aaaccgcccc ccaacaggga tttgatccaa 7140agtctgaaac ccctgttggt accgttgtga tcggcggtat tacgtacaac aggtatttgg 7200tcaaaggtaa ggaggttctg gttcccaagc ctgacaactg ccttgaagct gccaagctgt 7260cccttgagca agctctcgct gggatgggcc aaacttgtga ccttacagct gccgaggtgg 7320aaaagctaaa gcgcatcatt ggtcaacttc aaggattgac cactgagcag gctttaaact 7380gttagccgcc agcggcttga cccgctgtgg ccgcggcggc ctagttgtaa ctgaaacggc 7440ggtaaaaatt gtcaaatacc acagcagaac ttttacctta ggctctttag acctaaaagt 7500cacttccgag gtggaggtga agaagtcaac cgagcagggc cacgctgttg tggcaaactt 7560gtgttctggt gtcgtcttga tgagacctca cccaccgtcc cttgtcgacg ttcttctgaa 7620acccggactt gacataacac ccggcattca accagggcat ggggccggga atatgggcgt 7680ggacggttcc atttgggatt ttgaaaccgc acccacaaag gctgaactcg agttatccaa 7740gcaaataatt caagcatgtg aagtcaggcg cggggatgcc ccgaacctcc agctccctta 7800caagctctat cctgttagag gggatcctga gcggcataaa ggccacctca tcaataccag 7860gtttggagac ttaccttaca aaactcctca agacaccaag tccgcaatcc acgcggcttg 7920ttgcctgcac cccaacgggg cccccgtgtc tgatggtaaa tccacactag gtaccactct 7980tcaacatggc ttcgagcttt atgtccctac tgtgccctat agtgtcatgg agtaccttga 8040ttcacgccca gacacccctt ttatgtgcac taaacacggc acttccaagg ctgctgcaga 8100ggacctccaa aaatacgacc tatccaccca aggatttgtc ctgcctgggg tcctacgcct 8160agtgcgcagg ttcatctttg gccacattgg caaggcaccg ccattgttcc tcccatcaac 8220ttatcccgcc aagaactcca tggcaggtat taatggtcag aggttcccaa caaaggatgt 8280tcaaagtata cctgaaattg atgaaatgtg tgcccgcgcc gtcaaggaga attggcaaac 8340tgtgacacct tgcaccctca agaaacagta ttgttctagg cccaaaacca ggaccatcct 8400gggcactaac aacttcatag ccttggctca tagatcggcg ctcagtggtg ttacccaggc 8460attcatgaag aaggcttgga agtccccaat agccttaggg aaaaacaaat tcaaggagct 8520gcattgcact gtcgctggca ggtgcctcga ggccgacttg gcttcctgtg accgcagcac 8580ccctgccatt gtgaggtggt tcactaccca cctcctatat gaacttgcag gatgtgaaga 8640atatctacct agctatgtgc ttaactgttg ccatgacctt gtggcgacgc aggatggtgc 8700tttcacaaaa cgcggtggcc tgtcgtctgg agacccagtc accagtgtgt ccaacactgt

8760gtactcactg gtgatttatg cccagcacat ggtactatct gccctgaaaa tgggtcatga 8820aattggcctc aagttcctcg aagaacaact caaatttgag gaccttcttg aaatccagcc 8880tatgttagta tactctgatg atcttgtctt gtacgcagaa aagcccacct tccccaacta 8940tcattggtgg gtcgagcatc ttgacctgat gttgggcttt aaaacggacc caaagaaaac 9000cgtcataact gataaaccca gtttcctcgg gtgcagaatc gaagcagggc gacagctagt 9060ccccaatcgc gaccgcatcc tggctgctct tgcatatcac atgaaggcgc agaacgcctc 9120agagtattat gcgtctgctg ccgcaatcct gatggattca tgtgcttgca ttgaccacga 9180tcctgaatgg tatgaggacc tcatctgtgg cattgcccga tgcgctcgcc tggacggtta 9240tagctttcca ggtccggcat ttttcatgtc catgtgggag aagctgagga gtcataatga 9300agggaagaaa ttccgccact gcggcatctg cgacgccaaa gccgactacg cggctgcttg 9360tgggcttgat ttgtgtttgt ttcattcgca ctttcaccaa cactgccctg tcactctgag 9420ctgcggtcac catgccggtt cgaaggaatg ttcgcagtgt cagtcacctg ttggggccgg 9480cagatcccct cttgatgctg tgctggaaca aattccatac aaacctcctc gcactgtcat 9540catgaaggtg ggtaataaaa caacggccct tgatccgggg aggtaccagt cccgtcgagg 9600tctcgttgca gtcaagaggg gtattgcggg taatgaagtt gatcttgctg atggagatta 9660ccaagtggta cctcttctgc cgacttgcaa agatataaac atggtgaagg tggcttgcaa 9720cgtactactc agcaagttca tagtagggcc accaggttcc gggaagacca cctggctact 9780aagtcaagtt caggacgatg atgtcattta cacacccact catcagacca tgtttgacat 9840agttagtgct ctcaaagttt gcaggtattc cattccagga gcctcgggac tccctttccc 9900accgcctgcc aggtccgggc cgtgggttag gctcattgcc agcgggcacg tccctggccg 9960agtatcatac ctcgatgagg ccggatattg caatcatctg gacattctta gactgctttc 10020caaaacaccc cttgtgtgtt tgggtgacct tcagcaactt cacccagtcg gctttgattc 10080ctattgttat gtgttcgatc agatgcctca gaagcagttg accaccattt acagatttgg 10140ccccaacatc tgcgcagcca tccagccttg ttacagggag aaacttgaat ctaaggctag 10200gaacaccagg gtggttttta ccacccggcc tgtggccttt ggccaggtgc tgacaccata 10260tcacaaagat cgcgtcggct ccgcgattac catagactca tcccaggggg ccacctttga 10320cattgtaaca ttgcatctac catcgccaaa gtccctaaat aagtcccggg cacttgtggc 10380catcacacgg gcaagacacg ggttgttcat ttatgaccct cacaaccagc tccgggagtt 10440tttcaaccta acccctgagc gcactgattg taaccttgtg ttcagccgtg gagatgagct 10500ggtggtcctg aatgcagata atgcagtcac aaccgtggcg aaggccttag agacaggtcc 10560aactcaattt cgagtgtcag acccgaggtg caagtctctc ttagccgctt gctcggccag 10620tctggaaggg agctgcatgc cgctaccgca agtggcgcat aacctggggt tttacttctc 10680cccagacagt ccagtatttg cacctctgcc aaaagagttg gcgccacatt ggccagtggt 10740tacccatcag aataatcggg cgtggcctga tcgacttgtc gctagtatgc gtccaattga 10800cgcccgctac agcaagccga tggtcggtgc agggtatgtg gtcggaccgt ccaccttcct 10860tggtactcct ggagtggtgt catactatct cacactatac atcaggggtg agccccaggc 10920cttgccagaa acacttgttt caacaggacg tatagccaca gattgtcggg agtatctcga 10980cgcggctgag gaagaggcag caaaagaact tccccacgcg ttcattggcg atgtcaaagg 11040caccacagtt ggggggtgtc atcacattac atcaaaatac ctacctaggt ccctgcctaa 11100agactctgtt gccgtagttg gagtaagttc gcctggcagg gctgctaaag ccgtatgcac 11160cctcaccgat gtgtacctcc ctgaactccg gccatatctg caacctgaga cggcatcaaa 11220atgctggaaa ctcaaattag acttcaggga cgtccgacta atggtctgga aaggagccac 11280cgcctacttt caattggaag ggctcacatg gtcggcgctg cctgactatg ccaggtttat 11340tcagctgccc aaaaacgctg ttgtatacat cgatccgtgc ataggaccgg caacagccaa 11400tcgtaaagtc gtacgaacca cagattggcg ggccgacctg gcagtgacgc cgtatgatta 11460cggtgcccgg aacattttga caacagcctg gttcgaggac ctcgggccgc agtggaagat 11520tctggggttg cagcccttta ggcgggcgtt tggctttgaa aacactgagg attgggcaat 11580ccttgcatgc tgcatgagtg acggcaagga ctacactgac tataactgga attgcgttcg 11640acaacgccca cacgctatcc atggacgcgc tcgtgaccat acgtaccact ttgcccctgg 11700cactgaattg caagtggagc tcggtaaacc ccggctgcca cctgagcaag taccgtgaat 11760tcggagtgat gcaatggggt cactgtggag taaaatcagc cagctgttcg tggacgcctt 11820cactgaattc cttgttagtg tggttgatat tgtcatcttc cttgccatat tgtttgggtt 11880caccgtcgca ggatggttac tggtctttct tctcagagtg gtttgctccg cgcttctccg 11940ttcgcgctct gccattcact cttccgaact atcgaaggtc ctatgagggc ttactaccta 12000attgcagacc ggatgttcca caatttgcat ttaagcaccc tttgggtatg ttttggcaca 12060tgcgggtttc ccacctaatt gatcagatgg tctctcgccg catctaccag accatggaac 12120attcaggtca agcggcctgg aagcacgtgg tcagtgaggc tactcttaca aaattgtcag 12180aactcgacat agttctccac ttccaacacc tggccgcagt ggaggcggac tcttgtcgct 12240tcctcagctc acgacttgtg atgctgaaaa atcttgccgt tggcaatgtg agcttgcagt 12300acaacaccac gttgaaccgc gttgagctca tcctccccac accaggtacg aggcccaaat 12360tgaccgattt cagacaatgg ctcatcagtg tgcacgcttc cattttttcc tctgtagcct 12420catcagttac tttgttcata gtgctttggc ttcgaattcc agccgtacgc tatgtttttg 12480gtttccattg gcccatggca acacgtcatt cgagctgacc attaattaca ctatatgcat 12540gccctgtctt accagccaag cggctcaaca aaggctcgaa cccggtcgta acatgtggtg 12600caaaatagga cacaccacgt gcgaggagcg tgaccatgat gagttgtcaa tgtccatccc 12660gtccgggtac gacaacctca aacttgaagg ttattacgct tggctggctt ttttgtcctt 12720ttcctacgcg gcccaattcc atccggagtt gtttggaata gggaatgtgt cgcgcgtctt 12780tgtggataaa cgacaccagt tcatttgtgc cgagcatgac ggagataatt caaccgtatc 12840taccggacac aacatctccg catcatatgc ggcatattat caccaccaaa tagacggggg 12900caattggttc catttggaat ggctgcggcc gctcttttcc tcttggctag tgctcaacat 12960atcatggttt ctgaggcgtt cgcctgcaag ccctgtttct cgacgcatct atcagatatt 13020aagaccaata cgaccgcggc tgccggtttc atggtccttc aagacatcag ttgcctccga 13080cctcacaggg tctcagcatc gcaagagaac attcccttcg gaaagtcgtc acaatgtcgt 13140gaagccgtcg gtactcccca gtacattacg atgactgcta atgtgaccga cgaatcatat 13200ttgtacaacg cggacttgct aatgctttcc gcgtgccttt tccacgcctc agaaatgagc 13260gagaaaggct tcaaagttat ctttggaaac gtctccggcg ttgtttcagc ttgtgtcaat 13320ttcacagatt atgtggccca tgtaacccaa catacccaac agcatcatct ggtaattgat 13380cacattcggt tactgcattt cctgacacca tctgcaatga ggtgggctac aaccattgct 13440tgtttgttcg ccattctctt agcgatatga gatgttctca caaattgggg cgtttcttga 13500ctccgcactc ttgcttctgg tggctttttt tgctgtgtac cggcttgtcc tggtcctttg 13560ccgatggcaa cggcaacagc tcgacacgcc aatacatata taacttgacg atatgcgagc 13620tgaatgggac cgtctggttg tccagtcatt ttgattgggc agtcgagacc tttgtgcttt 13680acccggtggc cactcatatc ctctcactgg gttttctcac aacaagccat ttttttgatg 13740cgctcggtct cggcgctgtg tccactacgg gatttcttgg cgggcggtat gtacttagca 13800gcgtgtacgg cgcctgcgcc ttcgcagcgc ttgtatgttt tgtcatccgt gctgctaaaa 13860attgcatggc ttgccgttat gcccgcaccc ggttcaccaa cttcatcgtg gacgaccggg 13920ggaagatcca tcgatggaag tccccaatag tggtagagaa attaggcaaa gctgacatcg 13980gcggcgacct tgtcaccatc aaacatgttg tcctcgaagg agtcaaagct caacctttga 14040cgaggacatc ggcggagcaa tgggaagcct agatgatttt tgcaatgatc ctaccgccgc 14100acagaagctt gtgctggcat ttagtatcac atacacacct ataatgatat acgccctcaa 14160ggtgtcacgc ggccggctcc taggactgtt acacatcctg atatttctga actgttcttt 14220cacgttcgga tacatgacat acgtgcactt tcaatccact aaccgtgtcg cgcttactat 14280gggggcggtc gttgcccttt tgtggggcat ttacagcttt atagaatcat ggaagtttgt 14340cacttccaga tgcaggttgt gttgcctagg ccggcgatac attctggccc ctgcccacca 14400cgtagaaagt gctgcaggcc tccattcaat cccagcgtct ggtaaccgag catacgctgt 14460gagaaagccc ggactaacat cagtgaacgg cactctagta ccaggacttc ggagcctcgt 14520gttgggcggc aaacgagctg ttaaacgagg agtggttaac ctcgtcaagt atggccggta 14580aaaaccagag ccagaagaaa aagaaaaaca cagctcctat ggggagtggc cagccagtca 14640atcaactgtg ccaattgctg ggcacaatga taaagtccca gcgccagcgg cctaggggag 14700gacaggccaa aatgaaaaag cctgagaagc cacatttccc cctagctgct gaagatgaca 14760tccggcacca tttcacccag accgagcgtt ccctttgctt gcaatcgatc cagacggcct 14820tcaatcaagg cgcaggaact gcgtcgcttt catccagcgg gaaggtcagt tttcaggttg 14880agttcatgct gccggtcgct catacggtgc gcctgattcg cgtaacttcc acatccgcca 14940gtcagggtgc aagttaattt gatagttagg tgaatggccg cgattggcgt gtggcctctg 15000agtcacctat tcaattaggg cgatcacatg ggggttagac ttaattggcg agaaccatgt 15060gaccgaaatt aaaaaaaaaa aaaaaaaaaa aaaaaaaa 150983650PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(3)Xaa can be any naturally occurring amino acid 36Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa1 5 10 15Xaa Xaa Gly Xaa Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa 20 25 30Leu Glu Xaa Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 35 40 45Arg Ser 503749PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(3)Xaa can be any naturally occurring amino acid 37Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa1 5 10 15Xaa Xaa Gly Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu 20 25 30Glu Xaa Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg 35 40 45Ser 3848PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(3)Xaa can be any naturally occurring amino acid 38Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa1 5 10 15Xaa Xaa Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu 20 25 30Xaa Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 453947PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(3)Xaa can be any naturally occurring amino acid 39Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa1 5 10 15Xaa Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu Xaa 20 25 30Leu Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 454046PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(3)Xaa can be any naturally occurring amino acid 40Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa1 5 10 15Xaa Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu Xaa Leu 20 25 30Pro Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 454145PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(3)Xaa can be any naturally occurring amino acid 41Ser Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Asp Xaa Xaa1 5 10 15Met Trp Xaa Xaa Xaa Ser Xaa Xaa Ser Xaa Xaa Leu Glu Xaa Leu Pro 20 25 30Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Arg Ser 35 40 454246PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 42Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa1 5 10 15Xaa Xaa Xaa Ser Trp Ala Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa 20 25 30Xaa Xaa Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 40 454345PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 43Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa1 5 10 15Xaa Xaa Xaa Ser Trp Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa 20 25 30Xaa Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 40 454444PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 44Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa1 5 10 15Xaa Xaa Xaa Ser Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa 20 25 30Xaa Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 404543PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 45Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa1 5 10 15Xaa Xaa Xaa Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30Leu Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 404642PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 46Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa1 5 10 15Xaa Xaa Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa Leu 20 25 30Xaa Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 404741PRTPorcine reproductive and respiratory syndrome virusmisc_feature(2)..(2)Xaa can be any naturally occurring amino acid 47Ala Xaa Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Xaa Val Ala Xaa1 5 10 15Xaa Gly Gly Xaa Xaa Xaa Xaa Phe Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 20 25 30Leu Xaa Ala Xaa Xaa Xaa Xaa Xaa Ser 35 404850PRTPorcine reproductive and respiratory syndrome virusmisc_feature(17)..(17)Xaa can be any naturally occurring amino acid 48Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Xaa Asn Gly Arg Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa 20 25 30Leu Glu Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile 35 40 45Arg Ser 504949PRTPorcine reproductive and respiratory syndrome virusmisc_feature(17)..(17)Xaa can be any naturally occurring amino acid 49Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Xaa Asn Gly Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu 20 25 30Glu Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg 35 40 45Ser 5048PRTPorcine reproductive and respiratory syndrome virusmisc_feature(17)..(17)Xaa can be any naturally occurring amino acid 50Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Xaa Asn Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu 20 25 30Xaa Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 455147PRTPorcine reproductive and respiratory syndrome virusmisc_feature(17)..(17)Xaa can be any naturally occurring amino acid 51Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Xaa Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu Xaa 20 25 30Leu Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 455246PRTPorcine reproductive and respiratory syndrome virusmisc_feature(28)..(28)Xaa can be any naturally occurring amino acid 52Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Ser1 5 10 15Met Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu Xaa Leu 20 25 30Pro Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 455345PRTPorcine reproductive and respiratory syndrome virusmisc_feature(27)..(27)Xaa can be any naturally occurring amino acid 53Ser Ser Val Tyr Arg Trp Lys Lys Phe Val Val Phe Thr Asp Ser Met1 5 10 15Met Trp Thr Pro Glu Ser Asp Asp Ser Ala Xaa Leu Glu Xaa Leu Pro 20 25 30Pro Glu Leu Glu Arg Gln Val Glu Ile Leu Ile Arg Ser 35 40 455446PRTPorcine reproductive and respiratory syndrome virusmisc_feature(8)..(9)Xaa can be any naturally occurring amino acid 54Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa1 5 10 15Xaa Lys Val Ser Trp Ala Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val 20 25 30Pro Xaa Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40 455545PRTPorcine reproductive and respiratory syndrome virusmisc_feature(8)..(9)Xaa can be any naturally occurring amino acid 55Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa1 5 10 15Xaa Lys Val Ser Trp Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro 20 25 30Xaa Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40 455644PRTPorcine reproductive and respiratory syndrome virusmisc_feature(8)..(9)Xaa can be any naturally occurring amino acid 56Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa1 5 10 15Xaa Lys Val Ser Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro Xaa 20 25 30Glu Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 405743PRTPorcine reproductive and respiratory syndrome virusmisc_feature(8)..(9)Xaa can be any naturally occurring amino acid 57Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa1 5 10 15Xaa Lys Val Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro Xaa Glu 20 25 30Leu Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 405842PRTPorcine reproductive and respiratory syndrome virusmisc_feature(8)..(9)Xaa can be any naturally occurring amino acid 58Ala Thr Val Tyr Asp Ile Gly Xaa Xaa

Ala Val Met Tyr Val Ala Xaa1 5 10 15Xaa Lys Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro Xaa Glu Leu 20 25 30Lys Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 405941PRTPorcine reproductive and respiratory syndrome virusmisc_feature(8)..(9)Xaa can be any naturally occurring amino acid 59Ala Thr Val Tyr Asp Ile Gly Xaa Xaa Ala Val Met Tyr Val Ala Xaa1 5 10 15Xaa Gly Gly Xaa Glu Val Lys Phe Glu Xaa Val Pro Xaa Glu Leu Lys 20 25 30Leu Xaa Ala Asn Arg Leu Xaa Thr Ser 35 40

Claims

1. A Porcine Reproductive and Respiratory Syndrome (PRRS) virus comprising induction of interferon type I production and secretion by a cell infected by said virus.

2. The PRRS virus according to claim 1, wherein said virus comprises a modification in the gene encoding the nsp1 protein of said virus.

3. The PRRS virus according to claim 2, wherein said virus comprises a modification in the gene sequences encoding the nsp1.alpha. subunit and/or the nsp1.beta. subunit of the nsp1 protein (nsp1.alpha. and/or nsp1.beta.) of said virus.

4. The PRRS virus according to claim 2, wherein said virus comprises a modification in the gene sequence encoding the N-terminal domain (NTD) of the nsp1.beta. subunit of the nsp1 protein of said virus.

5. The PRRS virus according to claim 4, wherein the NTD starts with the N-terminus of nsp1.beta., preferably with the amino acid sequence SXXY if the PRRS virus is a genotype I PRRS virus or with the amino acid sequence AXVY if the PRRS virus is a genotype II PRRS virus and ends with the serine residue (S) within the amino acid sequence SFP of nsp1.beta..

6. The PRRS virus according to claim 2, wherein said modification comprises at least one mutation, deletion or insertion.

7. The PRRS virus according to claim 3, wherein said modification comprises at least one mutation, deletion or insertion.

8. The PRRS virus according to claim 4, wherein said mutation comprises at least one mutation, deletion or insertion.

9. The PRRS virus of according to claim 8, wherein said modification comprises a deletion or replacement of a nucleotide triplet coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.

10. The PRRS virus according to claim 9, wherein said modification comprises a deletion or replacement of at least 2 to 7 nucleotide triplets each coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.

11. The PRRS virus according to claim 9, wherein said modification comprises a deletion or replacement of more than 7 nucleotide triplets each coding for an amino acid residue located within the first 24 N-terminal amino acid residues of the NTD sequence.

12. The PRRS virus according to claim 10, wherein said modification comprises consecutive nucleotide triplets.

13. The PRRS virus according to claim 11, wherein said modification comprises consecutive nucleotide triplets.

14. The PRRS virus according to claim 9, wherein said modification comprises the deletion or replacement of a nucleotide triplet coding for an amino acid residue with a charged, side chain.

15. The PRRS virus according to claim 14, wherein said modification comprises the deletion or replacement of a nucleotide triplet coding for an amino acid residue with a positively charged, side chain.

16. The PRRS virus according to claim 9, wherein said modification comprises a deletion or replacement of the nucleotide triplet coding for the first arginine residue (R) located at least 21 amino acid residues in C-terminal direction from the N-terminal amino acid residue of the nsp1.beta. or the NTD.

17. The PRRS virus according to claim 16, wherein said modification comprises a deletion or replacement of the nucleotide triplet coding for the arginine residue (R) of the nsp1 amino acid sequence RXMW if the PRRS virus is a genotype I PRRS virus or with the amino acid sequence RGG of said virus if the PRRS virus is a genotype II PRRS virus.

18. The PRRS virus according to claim 16, wherein said modification comprises a deletion or replacement of the nucleotide triplet coding for the arginine residue (R) of the sequence RMM or RGG of the nsp1 protein of said virus.

19. The PRRS virus according to claim 16, wherein said modification comprises a deletion or replacement of one nucleotide triplet coding for the amino acid residue flanking said arginine residue (R) in N-terminal direction.

20. The PRRS virus according to claim 16, wherein said modification comprises a deletion or replacement of two consecutive nucleotide triplets coding for the amino acid residues PR.

21. The PRRS virus according to claim 19, wherein said modification further comprises a deletion or replacement of two, three, four, five, six, seven or more consecutive nucleotide triplets coding for two, three, four, five, six, seven or more amino acid residues flanking said arginine residue (R) in N-terminal direction.

22. The PRRS virus according to claim 21, wherein said modification comprises a deletion or replacement of three consecutive nucleotide triplets coding for the amino acid sequence RXR or APR of the nsp1.beta. subunit, or wherein said mutation comprises a deletion or replacement of four consecutive nucleotide triplets coding for the amino acid residues GRXR or WAPR of the nsp1.beta. subunit, wherein X is a genetically encoded amino acid residue.

23. The PRRS virus according to claim 2, wherein the modification comprises a deletion of the gene sequence encoding the entire nsp1 protein, encoding the entire nsp1.alpha. subunit, encoding the entire nsp1.beta. subunit, or encoding a at least seven amino acids of the entire NTD domain of the nsp1.beta. subunit.

24. The PRRS virus according to claim 2, wherein the nsp1 protein is a polypeptide having at least 70% sequence identity with a polypeptide selected from the group consisting of SEQ ID NOs 1-3.

25. The PRRS virus according to claim 3, wherein the nsp1.alpha. subunit is a polypeptide having at least 70% sequence identity with a polypeptide selected from the group consisting of SEQ ID NOs 4-6.

26. The PRRS virus according to claim 3 wherein the nsp1.beta. subunit is a polypeptide having at least 70% sequence identity with a polypeptide selected from the group consisting of SEQ ID NOs 7-9.

27. The PRRS virus according to claim 4, wherein the NTD domain of the nsp1.beta. subunit has a polypeptide sequence or is a polypeptide having at least 60% sequence identity with a polypeptide sequence or a polypeptide selected from the group consisting of SEQ ID NOs 10-12.

28. The PRRS virus according to claim 1, wherein said PPRS virus comprises a gene sequence coding for a polypeptide sequence or a polypeptide selected from the group consisting of SEQ ID NOs: 36-47.

29. The PRRS virus according to claim 1, wherein said PPRS virus comprises a gene sequence coding for a polypeptide sequence or a polypeptide selected from the group consisting of SEQ ID NOs: 48-59.

30. The PRRS virus according to claim 1, wherein said PPRS virus comprises a gene sequence coding for a polypeptide sequence or a polypeptide selected from the group consisting of SEQ ID NOs: 16-30.

31. The PRRS virus according to claim 1, wherein said PPRS virus comprises a RNA sequence complementary to a polynucleotide selected from the group consisting of SEQ ID NOs: 31-35.

32. The PRRS virus according to claim 1, wherein said PPRS virus has increased sensitivity to interferon type I.

33. The PRRS virus according to claim 1, wherein said PPRS virus is a live PRRS virus.

34. The PRRS virus according to claim 1, wherein said PPRS virus is a modified-live PRRS virus.

35. The PRRS virus according to claim 1, wherein said PRRS virus is a modified PRRS virus.

36. The PRRS virus according to claim 1, wherein said PRRS virus is attenuated PRRS virus.

37. The PRRS virus according to claim 1, wherein said PPRS virus is a genotype I or a genotype II PRRS virus.

38. The PRRS virus according to claim 1, wherein said interferon type I is Interferon-.alpha. and/or Interferon-.beta..

39. The PRRS virus according to claim 1, wherein said cell is a mammalian cell, and wherein said mammalian cell is a cell of a primary or secondary cell line and/or a host cell.

40. The PRRS virus according to claim 39, wherein said mammalian cell is a porcine cell or a simian cell.

41. The PRRS virus according to claim 40, wherein said porcine cell is a porcine macrophage

42. The PRRS virus according to claim 40, wherein said simian cell is a MA-104 or a MARC-145 cell.

43. A method for the treatment of Porcine Reproductive and Respiratory Syndrome comprising administering an effective amount of the PRRS virus of claim 1 to an animal in need of said treatment.

44. The method according to claim 43, where said animal is a swine, pig, piglet or a sow.

45. A polynucleotide comprising the genome of the PRRS virus according to claim 1.

46. A DNA Vector comprising a copy of the polynucleotide of claim 45.

47. A virus particle comprising the polynucleotide of claim 45 or 46.

48. A cell comprising the polynucleotide of claim 45 or the DNA-vector of claim 46.

49. A method of preparing a PRRS virus composition comprising the cultivation of the PRRS virus according to claim 1 in cell culture.

50. A method of producing the PRRS virus according to claim 1, comprising the mutagenesis of the gene coding for the nsp1 protein in the genome of a PRRS virus.

51. The method according to claim 51, wherein the modification according to claim 2 is introduced in the genome of said virus.

52. The method according to claim 51, wherein the vector of claim 46 is used.

53. The method according to claim 51, comprising the detection of interferon type I, or of mRNA coding for interferon type I.

54. The method of claim 53, wherein the interferon type I and/or the mRNA coding for interferon type I is detected by a bioassay, wherein said bioassay is preferably selected from the group consisting of ELISA, PCR, GLISA, IFA, biosensoric measurement, Surface Plasmon Resonance (SPR) measurement, selective media, lateral flow, biochip measurement, immunomagnetic separation, electrochemiluminescence, chromogenic media, immunodiffusion, DNA hybridization, staining, colorimetric detection, luminescence, and combinations thereof.

55. An immunogenic composition comprising the PRRS virus according to claim 1.

56. The immunogenic composition according to claim 55, comprising an amount of 10.sup.1 to 10.sup.7 viral particles per dose

57. The immunogenic composition according to claim 55, comprising an amount of 10.sup.3 to 10.sup.6 particles per dose.

58. The immunogenic composition according to claim 55, comprising an amount of 10.sup.4 to 10.sup.6 particles per dose.

59. The immunogenic composition according to claim 55, comprising an amount of said PRRS virus which is equivalent to a virus titre of at least about 10.sup.3 TCID₅₀/mL per dose.

60. The immunogenic composition according to claim 55, comprising an amount of said PRRS virus which is equivalent to a virus titre of between 10.sup.3 to 10.sup.5 TCID₅₀/mL per dose.

61. The immunogenic composition according to claim 55, further comprising one or more pharmaceutically acceptable carriers or excipients.

62. The immunogenic composition according to claim 61, wherein said one or more pharmaceutically acceptable carriers or excipients are selected from the group consisting of solvents, dispersion media, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonic agents, and adsorption delaying agents.

63. A method for inducing an immune response against PRRSV comprising administering an effective amount of the immunogenic composition of claim 55 to an animal in need thereof.

64. The method of claim 63, wherein the immunogenic composition is administered in a single dose or in two doses.

Images