POLYNUCLEOTIDES AND METHODS FOR THE IMPROVEMENT OF PLANTS

Abstract

The invention provides methods for producing a plant with altered seed yield, the methods comprising transformation of a plant with a genetic construct including a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID NO: 1 or a variant or fragment thereof. The method also provides isolated polypeptides, polynucleotides, constructs and vectors useful for producing a plant with altered seed yield. The method also provides plant cell and plants transformed to contain and express the polypeptides, polynucleotides and constructs. The invention also provides plants produced by methods of the invention.

Description

TECHNICAL FIELD

[0001] The present invention relates to compositions and methods for producing plants with increased seed yield.

BACKGROUND ART

[0002] As the population of the world increases, a major goal of agricultural research is to improve the grain yield of crop plant species.

[0003] Such improvements have until recently depended on selective breeding of plants for desirable characteristics. However for many plants the heterogeneous genetic compliments produced in off-spring do not result in the same desirable traits as those of their parents, thus limiting the effectiveness of selective breeding approaches.

[0004] Advances in molecular biology now make it possible to genetically manipulate the germplasm of both plants and animals. Genetic engineering of plants involves the isolation and manipulation of genetic material and the subsequent introduction of such material into a plant. This technology has led to the development of plants that are capable of expressing pharmaceuticals and other chemicals, plants with increased pest resistance, increased stress tolerance, and plants that express other beneficial traits.

[0005] Improvements in the grain yield of plant crop plants may be achieved by developing plants that produce more seed or grain than the equivalent wild-type plants.

[0006] Thus, there exists a need for plants with increased seed yield relative to their normally cultivated counterparts.

[0007] It is an object of the invention to provide improved compositions and/or methods for developing plant varieties with improved seed or grain seed yield or at least to provide the public with a useful choice.

SUMMARY OF THE INVENTION

[0008] In a first aspect the invention provides a method for producing a plant with altered seed yield, the method comprising transformation of a plant with: [0009] a) a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID. NO:1 or a variant of the polypeptide, wherein the variant is capable of modulating seed yield in a plant; [0010] b) a polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a), or [0011] c) a polynucleotide comprising a compliment, of at least 15 nucleotides in length, of the polynucleotide of a).

[0012] In one embodiment the variant has at least 70% sequence identity to a polypeptide with the amino acid sequence of SEQ ID NO: 1.

[0013] Preferably the variant is derived from a plant species and comprises the sequence of SEQ ID NO: 32.

[0014] In one embodiment the variant is from a monocotyledonous species and comprises the sequence of SEQ ID NO: 33.

[0015] In a preferred embodiment the variant comprises an amino acid sequence selected from any one of SEQ ID NO: 2-31.

[0016] In a preferred embodiment the polypeptide or variant is a glutamate decarboxylase.

[0017] In a preferred embodiment the polynucleotide of a) encodes a polypeptide with the amino acid sequence of SEQ ID NO: 1.

[0018] In a preferred embodiment the plant is transformed with a genetic construct containing the polynucleotide.

[0019] In a further aspect the invention provides a method of producing a plant with altered seed yield, the method comprising transformation of a plant cell or plant with: [0020] a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 34, or a variant thereof, wherein the variant encodes a polypeptide capable of modulating seed yield in a plant; [0021] b) a polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a), or [0022] c) a polynucleotide comprising a complement, of at least 15 nucleotides in length, of the polynucleotide of a).

[0023] In a one embodiment the variant comprises the sequence of any one of SEQ ID NO: 35 to 65.

[0024] In a preferred embodiment the polynucleotide or variant in a) encodes a polypeptide which is a glutamate decarboxylase.

[0025] In a further embodiment the polynucleotide of a) comprises the sequence of SEQ ID NO: 34.

[0026] In a further embodiment the polynucleotide of a) comprises the coding sequence of SEQ ID NO: 34.

[0027] In a preferred embodiment the plant is transformed with a genetic construct containing the polynucleotide.

[0028] Preferably the plant produced by the method of the invention has increased seed yield relative to a suitable control plant.

[0029] In a further embodiment the method for producing a plant with altered seed yield comprises transformation of a plant cell, or plant with a genetic construct capable or altering expression of a polypeptide which modulates seed yield.

[0030] In one embodiment, the method results in a plant with decreased seed yield, relative to a suitable control, due to transformation of a plant cell or plant, with a genetic construct capable of down-regulating expression of a polypeptide which positively modulates seed yield.

[0031] In a preferred embodiment, the method results in a plant with increased seed yield, relative to a suitable control, due to transformation of a plant cell or plant, with a genetic construct capable of up-regulating expression of a polypeptide which positively modulates seed yield.

[0032] In a further aspect the invention provides a plant cell or plant produced by a method of the invention.

[0033] Preferably the plant produced by the method of the invention has increased seed yield relative to a suitable control plant.

[0034] In a further aspect the invention provides an isolated polynucleotide having at least 75% sequence identity to a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence selected of SEQ ID NO: 1, wherein the polynucleotide encodes a polypeptide capable of modulating seed yield in a plant.

[0035] In one embodiment said nucleotide sequence comprises the sequence of SEQ ID NO: 34.

[0036] In a further embodiment said nucleotide sequence comprises the full-length coding sequence of SEQ ID NO:34.

[0037] In a further aspect the invention provides a polynucleotide encoding a polypeptide with at least 70% identity to the amino acid sequence of SEQ ID NO: 1.

[0038] Preferably the polypeptide is capable of modulating seed yield in a plant.

[0039] Preferably the polypeptide is a glutamate decarboxylase.

[0040] In a further aspect the invention provides an isolated polynucleotide that encodes a polypeptide comprising an amino acid sequence SEQ ID NO: 1.

[0041] In a further embodiment the polynucleotide comprises the sequence of SEQ ID NO: 34.

[0042] In a further embodiment the polynucleotide comprises the full-length coding sequence of SEQ ID NO: 34.

[0043] In a further aspect the invention provides an isolated polynucleotide comprising the sequence of SEQ ID NO: 34 or a variant thereof, wherein the variant is from ryegrass or fescue, and encodes a polypeptide capable of modulating seed yield in a plant.

[0044] In one embodiment the polypeptide is a glutamate decarboxylase.

[0045] In one embodiment the isolated polynucleotide comprises the sequence of SEQ ID NO: 34.

[0046] In a further aspect the invention provides an isolated polypeptide having at least 83% sequence identity to the amino acid sequence of SEQ ID NO: 1, wherein the polypeptide is capable of modulating seed yield in a plant.

[0047] In one embodiment the isolated polypeptide of comprises the amino acid sequence of SEQ ID NO: 1.

[0048] In a further aspect the invention provides an isolated polynucleotide encoding a polypeptide of the invention.

[0049] In a further aspect the invention provides an isolated polynucleotide comprising: [0050] a) a polynucleotide comprising a fragment, of at least 15 nucleotides in length, of a polynucleotide of the invention; [0051] b) a polynucleotide comprising a complement, of at least 15 nucleotides in length, of the polynucleotide of the invention; or [0052] c) a polynucleotide comprising a sequence, of at least 15 nucleotides in length, capable of hybridising to the polynucleotide of the invention.

[0053] In a further aspect the invention provides a genetic construct which comprises a polynucleotide of the invention.

[0054] In one embodiment the genetic construct is an expression construct.

[0055] In one embodiment the construct comprises a promoter operably linked to the polynucleotide.

[0056] In a further embodiment the construct comprises a terminator operably linked to the polynucleotide.

[0057] Preferably the promoter, the terminator, or both is/are derived from a different source than the polynucleotide.

[0058] In one embodiment the different source is a different species:

[0059] In a further aspect the invention provides a vector comprising a genetic construct or expression construct of the invention.

[0060] In a further aspect the invention provides a host cell genetically modified, to express a polynucleotide of the invention, or a polypeptide of the invention.

[0061] In a further aspect the invention provides a host cell comprising a genetic construct or expression construct of the invention.

[0062] In a further aspect the invention provides a plant cell genetically modified to express a polynucleotide of the invention, or a polypeptide of the invention.

[0063] In a further aspect the invention provides a plant cell which comprises a genetic construct of the invention or the expression construct of the invention.

[0064] In a further aspect the invention provides a plant which comprises a plant cell of the invention.

[0065] In a further aspect the invention provides a method for selecting a plant with altered seed yield relative to suitable contrast, the method comprising testing of a plant for altered expression of a polynucleotide of the invention.

[0066] In a further aspect the invention provides a method for selecting a plant with altered seed yield relative to a suitable control, the method comprising testing of a plant for altered expression of a polypeptide of the invention.

[0067] In a further aspect the invention provides a plant cell or plant produced by the method of the invention.

[0068] In a further aspect the invention provides a plant selected by the method of the invention.

[0069] In a further aspect the invention provides a population or group of plants selected by the method of the invention.

[0070] In a further aspect the invention provides an antibody raised against a polypeptide of the invention.

[0071] The polynucleotides and polynucleotide variants of the invention may be derived from any species and/or may be produced synthetically or recombinantly.

[0072] In one embodiment the polynucleotide or variant, is derived from a plant species.

[0073] In a further embodiment the polynucleotide or variant, is derived from a gymnosperm plant species.

[0074] In a further embodiment the polynucleotide or variant, is derived from an angiosperm plant species.

[0075] In a further embodiment the polynucleotide or variant, is derived from a from dicotyledonous plant species.

[0076] In a further embodiment the polynucleotide or variant, is derived from a monocotylenouous plant species.

[0077] The polypeptide and polypeptide variants, of the invention may be derived from any species and/or may be produced synthetically or recombinantly.

[0078] In one embodiment the polypeptide or variant, is derived from a plant species.

[0079] In a further embodiment the polypeptide or variant, is derived from a gymnosperm plant species.

[0080] In a further embodiment the polypeptide or variant, is derived from an angiosperm plant species.

[0081] In a further embodiment the polypeptide or variant, is derived from a from dicotyledonous plant species.

[0082] In a further embodiment the polypeptide or variant, is derived from a monocotylenouous plant species.

[0083] The plant cells and plants, of the invention may be derived from any species.

[0084] In one embodiment the plant cell or plant, is derived from a gymnosperm plant species.

[0085] In a further embodiment the plant cell or plant, is derived from an angiosperm plant species.

[0086] In a further embodiment the plant cell or plant, is derived from a from dicotyledonous plant species.

[0087] In a further embodiment the plant cell or plant, is derived from a monocotylenouous plant species.

[0088] Preferred dicotyledonous genera include: Amygdalus, Anacardium, Arachis, Brassica, Cajanus, Cannabis, Carthamus, Carya, Ceiba, Cicer, Cocos, Coriandrum, Coronilla, Cossypium, Crotalaria, Dolichos, Elaeis, lycine, Gossypium, Helianthus, Lathyrus, Lens, Lespedeza, Linum, Lotus, Lupinus, Macadamia, Medicago, Melilotus, Mucuna, Olea, Onobrychis, Ornithopus, Papaver, Phaseolus, Phoenix, Pistacia, Pisum, Prunus, Pueraria, Ribes, Ricinus, Sesamum, Theobroma, Trifolium, Trigonella, Vicia and Vigna.

[0089] Preferred dicotyledonous species include: Amygdalus communis, Anacardium occidentale, Arachis hypogaea, Arachis hypogea, Brassica napus Rape, Brassica nigra. Brassica campestris, Cajanus cajan, Cajanus indicus, Cannabis sativa, Carthamus tinctorius, Carya illinoinensis, Ceiba pentandra, Cicer arietinum, Cocos nucifera, Coriandrum sativum, Coronilla varia, Cossypium hirsutum, Crotalaria juncea, Dolichos lablab, Elaeis guineensis, Gossypium arboreum, Gossypium nanking, Gossypium barbadense, Gossypium herbaceum, Gossypium hirsutum, Glycine max, Glycine ussuriensis, Glycine gracilis, Helianthus annus, Lathyrus angustifolius, Lathyrus luteus, Lathyrus mutabilis, Lathyrus sericea, Lathyrus striata, Lathyrus uliginosus, Lathyrus sativus, Lens culinaris, Lespedeza stipulacea, Linum usitatissimum, Lotus corniculatus, Lupinus albus, Medicago arabica, Medicago arborea, Medicago falcate, Medicago hispida, Medicago officinalis, Medicago sativa, Medicago tribuloides, Macadamia integrifolia, Melilotus albus, Mucuna pruriens, Olea europaea, Onobrychis viciifolia, Ornithopus sativus, Phaseolus aureus, Phaseolus aureus cerasifera, Phaseolus aureus cerasus, Phaseolus aureus coccineus, Phaseolus aureus domestica, Phaseolus aureus lunatus, Phaseolus aureus maheleb, Phaseolus aureus mungo, Phaseolus aureus persica, Phaseolus aureus pseudocerasus, Phaseolus aureus vulgaris, Papaver somniferum, Phaseolus acutifolius, Phoenix dactylifera, Pistacia vera, Pisum sativum, Prunus amygdalus, Prunus armeniaca, Pueraria thunbergiana, Ribes nigrum, Ribes rubrum, Ribes grossularia, Ricinus communis, Sesamum indicum, Trifolium augustifolium, Trifolium diffusum, Trifolium hybridum, Trifolium incarnatum, Trifolium ingrescens, Trifolium pratense, Trifolium repens, Trifolium resupinatum, Trifolium subterraneum, Theobroma cacao, Trifolium alexandrinum, Trigonella foenumgraecum, Vigna angustifolia, Vigna atropurpurea, Vigna calcarata, Vigna dasycarpa, Vigna ervilia, Vigna oxycoccos, Vigna pannonica, Vigna sesquipedalis, Vigna sinensis, Vigna villosa, Vicia faba, Vicia sative and Vigna angularis.

[0090] Preferred monocotyledonous genera include: Agropyron, Allium, Alopecurus, Andropogon, Arrhenatherum, Asparagus, Avena, Bambusa, Bothrichloa, Bouteloua, Bromus, Cenchrus, Chloris, Cymbopogon, Cynodon, Dactylis, Dichanthium, Digitaria, Eleusine, Elymus, Eragrostis, Fagopyrum, Festuca, Hordeum, Lolium, Oryza, Panicum, Paspalum, Pennisetum, Phalaris, Phleum, Poa, Saccharum, Secale, Setaria, Sorghastrum, Sorghum, Triticum, Vanilla, x Triticosecale and Zea.

[0091] Preferred monocotyledonous species include: Agropyron desertorum, Agropyron elongatum, Agropyron spicatum, Agropyron trachycaulum, Agropyron trichophorum, Allium fistulosum, Allium sativum, Alopecurus pratensis, Andropogon gerardi, Arrhenatherum elatius, Asparagus officinalis, Avena sativa, Bambusa vulgaris, Bothrichloa barbinodis, Bothrichloa ischaemum, Bouteloua curipendula, Bouteloua gracilis, Bromus erectus, Cenchrus ciliaris, Chloris gayana, Cymbopogon nardus, Cynodon dactylon, Dactylis glomerata, Dichanthium annulatum, Digitaria decumbens, Eleusine coracan, Elymus angustus, Eragrostis curvula, Eragrostis tef, Fagopyrum esculentum, Fagopyrum tataricum, Festuca arundinacea, Hordeum distichum, Hordeum vulgare, Lolium perenne, Lolium multiflorum, Oryza sativa, Panicum italicium, Panicum maximum, Panicum miliaceum, Paspalum dilatatum, Pennisetum clandestinum, Pennisetum glaucum, Phalaris arundinacea, Phleum bertolinii, Poa fendleriana, Poa nemoralis, Saccharum robustum, Saccharum sinense, Secale cereale, Setaria sphacelata, Sorghastrum nutans, Sorghum dochna, Sorghum halepense, Sorghum bicolor, Triticum aestivum, Triticum dicoccum, X Triticosecale, Zea mays, Agropyron cristatum, Agropyron intermedium, Agropyron smithii, Allium ascalonicum, Allium cepa, Allium chinense, Allium porrum, Allium schoenoprasum, Avena nuda, Bambusa vulgaris, Bothrichloa saccharoides, Bouteloua eriopoda, Bromus inermis, Bromus riparius, Dactylis aristatum, Dactylis sericeum, Digitaria smutsii, Elymus junceus, Festuca ovina, Festuca pratensis, Festuca rubra, Panicum purpurascens, Panicum virgatum, Paspalum notatum, Pennisetum purpureum, Pennisetum spicatum, Phleum pratense, Poa pratensis, Saccharum officinarum, Saccharum spontaneum, Sorghum sudanense, Triticum durum, Triticum monococcum, Vanilla fragrans and Zea mays.

[0092] Preferred plants are from the genera Lolium and Trifolium. Particularly preferred are the species Lolium perenne and Trifolium repens.

[0093] Particularly preferred monocotyledonous plant species are: Lolium perenne and Oryza sativa.

[0094] The term "plant" is intended to include a whole plant, any part of a plant, propagules and progeny of a plant.

[0095] The term `propagule` means any part of a plant that may be used in reproduction or propagation, either sexual or asexual, including seeds and cuttings.

DETAILED DESCRIPTION

General Definitions

[0096] The term "comprising" as used in this specification means "consisting at least in part of". When interpreting each statement in this specification that includes the term "comprising", features other than that or those prefaced by the term may also be present. Related terms such as "comprise" and "comprises" are to be interpreted in the same manner.

[0097] In this specification where reference has been made to patent specifications, other external documents, or other sources of information, this is generally for the purpose of providing a context for discussing the features of the invention. Unless specifically stated otherwise, reference to such external documents is not to be construed as an admission that such documents, or such sources of information, in any jurisdiction, are prior art, or form part of the common general knowledge in the art.

Polynucleotides and Fragments

[0098] The term "polynucleotide(s)," as used herein, means a single or double-stranded deoxyribonucleotide or ribonucleotide polymer of any length but preferably at least 15 nucleotides, and include as non-limiting examples, coding and non-coding sequences of a gene, sense and antisense sequences complements, exons, introns, genomic DNA, cDNA, pre-mRNA, mRNA, rRNA, siRNA, miRNA, tRNA, ribozymes, recombinant polypeptides, isolated and purified naturally occurring DNA or RNA sequences, synthetic RNA and DNA sequences, nucleic acid probes, primers and fragments.

[0099] A "fragment" of a polynucleotide sequence provided herein is a subsequence of contiguous nucleotides that is capable of specific hybridization to a target of interest, e.g., a sequence that is at least 15 nucleotides in length. The fragments of the invention comprise 15 nucleotides, preferably at least 20 nucleotides, more preferably at least 30 nucleotides, more preferably at least 50 nucleotides, more preferably at least 50 nucleotides and most preferably at least 60 nucleotides of contiguous nucleotides of a polynucleotide of the invention. A fragment of a polynucleotide sequence can be used in antisense, gene silencing, triple helix or ribozyme technology, or as a primer, a probe, included in a microarray, or used in polynucleotide-based selection methods of the invention.

[0100] The term "primer" refers to a short polynucleotide, usually having a free 3'OH group, that is hybridized to a template and used for priming polymerization of a polynucleotide complementary to the target.

[0101] The term "probe" refers to a short polynucleotide that is used to detect a polynucleotide sequence, that is complementary to the probe, in a hybridization-based assay. The probe may consist of a "fragment" of a polynucleotide as defined herein.

Polypeptides and Fragments

[0102] The term "polypeptide", as used herein, encompasses amino acid chains of any length but preferably at least 5 amino acids, including full-length proteins, in which amino acid residues are linked by covalent peptide bonds. Polypeptides of the present invention may be purified natural products, or may be produced partially or wholly using recombinant or synthetic techniques. The term may refer to a polypeptide, an aggregate of a polypeptide such as a dimer or other multimer, a fusion polypeptide, a polypeptide fragment, a polypeptide variant, or derivative thereof.

[0103] A "fragment" of a polypeptide is a subsequence of the polypeptide that performs a function that is required for the biological activity and/or provides three dimensional structure of the polypeptide. The term may refer to a polypeptide, an aggregate of a polypeptide such as a dimer or other multimer, a fusion polypeptide, a polypeptide fragment, a polypeptide variant, or derivative thereof capable of performing the above enzymatic activity.

[0104] The term "isolated" as applied to the polynucleotide or polypeptide sequences disclosed herein is used to refer to sequences that are removed from their natural cellular environment. An isolated molecule may be obtained by any method or combination of methods including biochemical, recombinant, and synthetic techniques.

[0105] The term "recombinant" refers to a polynucleotide sequence that is removed from sequences that surround it in its natural context and/or is recombined with sequences that are not present in its natural context.

[0106] A "recombinant" polypeptide sequence is produced by translation from a "recombinant" polynucleotide sequence.

[0107] The term "derived from" with respect to polynucleotides or polypeptides of the invention being derived from a particular genera or species, means that the polynucleotide or polypeptide has the same sequence as a polynucleotide or polypeptide found naturally in that genera or species. The polynucleotide or polypeptide, derived from a particular genera or species, may therefore be produced synthetically or recombinantly.

Variants

[0108] As used herein, the term "variant" refers to polynucleotide or polypeptide sequences different from the specifically identified sequences, wherein one or more nucleotides or amino acid residues is deleted, substituted, or added. Variants may be naturally occurring allelic variants, or non-naturally occurring variants. Variants may be from the same or from other species and may encompass homologues, paralogues and orthologues. In certain embodiments, variants of the inventive polypeptides and polypeptides possess biological activities that are the same or similar to those of the inventive polypeptides or polypeptides. The term "variant" with reference to polypeptides and polypeptides encompasses all forms of polypeptides and polypeptides as defined herein.

Polynucleotide Variants

[0109] Variant polynucleotide sequences preferably exhibit at least 50%, more preferably at least 51%, more preferably at least 52%, more preferably at least 53%, more preferably at least 54%, more preferably at least 55%, more preferably at least 56%, more preferably at least 57%, more preferably at least 58%, more preferably at least 59%, more preferably at least 60%, more preferably at least 61%, more preferably at least 62%, more preferably at least 63%, more preferably at least 64%, more preferably at least 65%, more preferably at least 66%, more preferably at least 67%, more preferably at least 68%, more preferably at least 69%, more preferably at least 70%, more preferably at least 71%, more preferably at least 72%, more preferably at least 73%, more preferably at least 74%, more preferably at least 75%, more preferably at least 76%, more preferably at least 77%, more preferably at least 78%, more preferably at least 79%, more preferably at least 80%, more preferably at least 81%, more preferably at least 82%, more preferably at least 83%, more preferably at least 84%, more preferably at least 85%, more preferably at least 86%, more preferably at least 87%, more preferably at least 88%, more preferably at least 89%, more preferably at least 90%, more preferably at least 91%, more preferably at least 92%, more preferably at least 93%, more preferably at least 94%, more preferably at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, and most preferably at least 99% identity to a specified polynucleotide sequence. Identity is found over a comparison window of at least 20 nucleotide positions, preferably at least 50 nucleotide positions, more preferably at least 100 nucleotide positions, and most preferably over the entire length of the specified polynucleotide sequence.

[0110] Polynucleotide sequence identity can be determined in the following manner. The subject polynucleotide sequence is compared to a candidate polynucleotide sequence using BLASTN (from the BLAST suite of programs, version 2.2.5 [Nov. 2002]) in bl2seq (Tatiana A. Tatusova, Thomas L. Madden (1999), "Blast 2 sequences--a new tool for comparing protein and nucleotide sequences", FEMS Microbiol Lett. 174:247-250), which is publicly available from NCBI (ftp://ftp.ncbi.nih.gov/blast/). The default parameters of bl2seq are utilized except that filtering of low complexity parts should be turned off.

[0111] The identity of polynucleotide sequences may be examined using the following unix command line parameters:

bl2seq -i nucleotideseq1 -j nucleotideseq2 -F F -p blastn

[0112] The parameter -F F turns off filtering of low complexity sections. The parameter -p selects the appropriate algorithm for the pair of sequences. The bl2seq program reports sequence identity as both the number and percentage of identical nucleotides in a line "Identities=".

[0113] Polynucleotide sequence identity may also be calculated over the entire length of the overlap between a candidate and subject polynucleotide sequences using global sequence alignment programs (e.g. Needleman, S. B. and Wunsch, C. D. (1970) J. Mol. Biol. 48, 443-453). A full implementation of the Needleman-Wunsch global alignment algorithm is found in the needle program in the EMBOSS package (Rice, P. Longden, I. and Bleasby, A. EMBOSS: The European Molecular Biology Open Software Suite, Trends in Genetics June 2000, vol 16, No 6. pp. 276-277) which can be obtained from http://www.hgmp.mrc.ac.uk/Software/EMBOSS/. The European Bioinformatics Institute server also provides the facility to perform EMBOSS-needle global alignments between two sequences on line at http:/www.ebi.ac.uk/emboss/align/.

[0114] Alternatively the GAP program may be used which computes an optimal global alignment of two sequences without penalizing terminal gaps. GAP is described in the following paper: Huang, X. (1994) On Global Sequence Alignment. Computer Applications in the Biosciences 10, 227-235.

[0115] Polynucleotide variants of the present invention also encompass those which exhibit a similarity to one or more of the specifically identified sequences that is likely to preserve the functional equivalence of those sequences and which could not reasonably be expected to have occurred by random chance. Such sequence similarity with respect to polypeptides may be determined using the publicly available bl2seq program from the BLAST suite of programs (version 2.2.5 [Nov. 2002]) from NCBI (ftp://ftp.ncbi.nih.gov/blast/).

[0116] The similarity of polynucleotide sequences may be examined using the following unix command line parameters:

[0117] bl2seq -i nucleotideseq1 -j nucleotideseq2 -F F -p tblastx

[0118] The parameter -F F turns off filtering of low complexity sections. The parameter -p selects the appropriate algorithm for the pair of sequences. This program finds regions of similarity between the sequences and for each such region reports an "E value" which is the expected number of times one could expect to see such a match by chance in a database of a fixed reference size containing random sequences. The size of this database is set by default in the bl2seq program. For small E values, much less than one, the E value is approximately the probability of such a random match.

[0119] Variant polynucleotide sequences preferably exhibit an E value of less than 1×10^-10 more preferably less than 1×10^-20, more preferably less than 1×10^-30, more preferably less than 1×10^-40, more preferably less than 1×10^-50, more preferably less than 1×10^-60, more preferably less than 1×10^-70, more preferably less than 1×10^-80, more preferably less than 1×10^-90, more preferably less than 1×10^-100, more preferably less than 1×10^-110, and most preferably less than 1×10^-120 when compared with any one of the specifically identified sequences.

[0120] Alternatively, variant polynucleotides of the present invention hybridize to a specified polynucleotide sequence, or complements thereof under stringent conditions.

[0121] The term "hybridize under stringent conditions", and grammatical equivalents thereof, refers to the ability of a polynucleotide molecule to hybridize to a target polynucleotide molecule (such as a target polynucleotide molecule immobilized on a DNA or RNA blot, such as a Southern blot or Northern blot) under defined conditions of temperature and salt concentration. The ability to hybridize under stringent hybridization conditions can be determined by initially hybridizing under less stringent conditions then increasing the stringency to the desired stringency.

[0122] With respect to polynucleotide molecules greater than about 100 bases in length, typical stringent hybridization conditions are no more than 25 to 30° C. (for example, 10° C.) below the melting temperature (Tm) of the native duplex (see generally, Sambrook et al., Eds, 1987, Molecular Cloning, A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press; Ausubel et al., 1987, Current Protocols in Molecular Biology, Greene Publishing,). Tm for polynucleotide molecules greater than about 100 bases can be calculated by the formula Tm=81.5+0.41% (G+C-log(Na+). (Sambrook et al., Eds, 1987, Molecular Cloning, A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press; Bolton and McCarthy, 1962, PNAS 84:1390). Typical stringent conditions for polynucleotide of greater than 100 bases in length would be hybridization conditions such as prewashing in a solution of 6×SSC, 0.2% SDS; hybridizing at 65° C., 6×SSC, 0.2% SDS overnight; followed by two washes of 30 minutes each in 1×SSC, 0.1% SDS at 65° C. and two washes of 30 minutes each in 0.2×SSC, 0.1% SDS at 65° C.

[0123] With respect to polynucleotide molecules having a length less than 100 bases, exemplary stringent hybridization conditions are 5 to 10° C. below Tm. On average, the Tm of a polynucleotide molecule of length less than 100 by is reduced by approximately (500/oligonucleotide length)° C.

[0124] With respect to the DNA mimics known as peptide nucleic acids (PNAs) (Nielsen et al., Science. 1991 Dec. 6; 254(5037):1497-500) Tm values are higher than those for DNA-DNA or DNA-RNA hybrids, and can be calculated using the formula described in Giesen et al., Nucleic Acids Res. 1998 Nov. 1; 26(21):5004-6. Exemplary stringent hybridization conditions for a DNA-PNA hybrid having a length less than 100 bases are 5 to 10° C. below the Tm.

[0125] Variant polynucleotides of the present invention also encompasses polynucleotides that differ from the sequences of the invention but that, as a consequence of the degeneracy of the genetic code, encode a polypeptide having similar activity to a polypeptide encoded by a polynucleotide of the present invention. A sequence alteration that does not change the amino acid sequence of the polypeptide is a "silent variation". Except for ATG (methionine) and TGG (tryptophan), other codons for the same amino acid may be changed by art recognized techniques, e.g., to optimize codon expression in a particular host organism.

[0126] Polynucleotide sequence alterations resulting in conservative substitutions of one or several amino acids in the encoded polypeptide sequence without significantly altering its biological activity are also included in the invention. A skilled artisan will be aware of methods for making phenotypically silent amino acid substitutions (see, e.g., Bowie et al., 1990, Science 247, 1306).

[0127] Variant polynucleotides due to silent variations and conservative substitutions in the encoded polypeptide sequence may be determined using the publicly available bl2seq program from the BLAST suite of programs (version 2.2.5 [Nov. 2002]) from NCBI (ftp://ftp.ncbi.nih.gov/blast/) via the tblastx algorithm as previously described.

Polypeptide Variants

[0128] The term "variant" with reference to polypeptides encompasses naturally occurring, recombinantly and synthetically produced polypeptides. Variant polypeptide sequences preferably exhibit at least 50%, more preferably at least 51%, more preferably at least 52%, more preferably at least 53%, more preferably at least 54%, more preferably at least 55%, more preferably at least 56%, more preferably at least 57%, more preferably at least 58%, more preferably at least 59%, more preferably at least 60%, more preferably at least 61%, more preferably at least 62%, more preferably at least 63%, more preferably at least 64%, more preferably at least 65%, more preferably at least 66%, more preferably at least 67%, more preferably at least 68%, more preferably at least 69%, more preferably at least 70%, more preferably at least 71%, more preferably at least 72%, more preferably at least 73%, more preferably at least 74%, more preferably at least 75%, more preferably at least 76%, more preferably at least 77%, more preferably at least 78%, more preferably at least 79%, more preferably at least 80%, more preferably at least 81%, more preferably at least 82%, more preferably at least 83%, more preferably at least 84%, more preferably at least 85%, more preferably at least 86%, more preferably at least 87%, more preferably at least 88%, more preferably at least 89%, more preferably at least 90%, more preferably at least 91%, more preferably at least 92%, more preferably at least 93%, more preferably at least 94%, more preferably at least 95%, more preferably at least 96%, more preferably at least 97%, more preferably at least 98%, and most preferably at least 99% identity to a sequences of the present invention. Identity is found over a comparison window of at least 20 amino acid positions, preferably at least 50 amino acid positions, more preferably at least 100 amino acid positions, and most preferably over the entire length of a polypeptide of the invention.

[0129] Polypeptide sequence identity can be determined in the following manner. The subject polypeptide sequence is compared to a candidate polypeptide sequence using BLASTP (from the BLAST suite of programs, version 2.2.5 [Nov. 2002]) in bl2seq, which is publicly available from NCBI (ftp://ftp.ncbi.nih.gov/blast/). The default parameters of bl2seq are utilized except that filtering of low complexity regions should be turned off.

[0130] Polypeptide sequence identity may also be calculated over the entire length of the overlap between a candidate and subject polynucleotide sequences using global sequence alignment programs. EMBOSS-needle (available at http:/www.ebi.ac.uk/emboss/align/) and GAP (Huang, X. (1994) On Global Sequence Alignment. Computer Applications in the Biosciences 10, 227-235.) as discussed above are also suitable global sequence alignment programs for calculating polypeptide sequence identity.

[0131] Use of BLASTP as described above is preferred for use in the determination of polypeptide variants according to the present invention.

[0132] Polypeptide variants of the present invention also encompass those which exhibit a similarity to one or more of the specifically identified sequences that is likely to preserve the functional equivalence of those sequences and which could not reasonably be expected to have occurred by random chance. Such sequence similarity with respect to polypeptides may be determined using the publicly available bl2seq program from the BLAST suite of programs (version 2.2.5 [Nov. 2002]) from NCBI (ftp://ftp.ncbi.nih.gov/blast/). The similarity of polypeptide sequences may be examined using the following unix command line parameters:

[0133] bl2seq -i peptideseq1 -j peptideseq2 -F F -p blastp

[0134] Variant polypeptide sequences preferably exhibit an E value of less than 1×10^-10 more preferably less than 1×10^-20, more preferably less than 1×10^-30, more preferably less than 1×10^-40, more preferably less than 1×10^-50, more preferably less than 1×10^-60, more preferably less than 1×10^-70, more preferably less than 1×10^-80, more preferably less than 1×10^-90, more preferably less than 1×10^-100 more preferably less than 1×10^-110 more preferably less than 1×10^-120 more preferably less than 1×10^-130, more preferably less than 1×10^-140 more preferably less than 1×10^-150, more preferably less than 1×10^-160, more preferably less than 1×10^-170, more preferably less than 1×10^-180, more preferably less than 1×10^-190, more preferably less than 1×10^-200, more preferably less than 1×10^-210, more preferably less than 1×10^-220, and most preferably less than 1×10^-222 when compared with any one of the specifically identified sequences.

[0135] The parameter -F F turns off filtering of low complexity sections. The parameter -p selects the appropriate algorithm for the pair of sequences. This program finds regions of similarity between the sequences and for each such region reports an "E value" which is the expected number of times one could expect to see such a match by chance in a database of a fixed reference size containing random sequences. For small E values, much less than one, this is approximately the probability of such a random match.

[0136] Conservative substitutions of one or several amino acids of a described polypeptide sequence without significantly altering its biological activity are also included in the invention. A skilled artisan will be aware of methods for making phenotypically silent amino acid substitutions (see, e.g., Bowie et al., 1990, Science 247, 1306).

Constructs, Vectors and Components Thereof

[0137] The term "genetic construct" refers to a polynucleotide molecule, usually double-stranded DNA; which may have inserted into it another polynucleotide molecule (the insert polynucleotide molecule) such as, but not limited to, a cDNA molecule. A genetic construct may contain the necessary elements that permit transcribing the insert polynucleotide molecule, and, optionally, translating the transcript into a polypeptide. The insert polynucleotide molecule may be derived from the host cell, or may be derived from a different cell or organism and/or may be a recombinant polynucleotide. Once inside the host cell the genetic construct may become integrated in the host chromosomal DNA. The genetic construct may be linked to a vector.

[0138] The term "vector" refers to a polynucleotide molecule, usually double stranded DNA, which is used to transport the genetic construct into a host cell. The vector may be capable of replication in at least one additional host system, such as E. coli.

[0139] The term "expression construct" refers to a genetic construct that includes the necessary elements that permit transcribing the insert polynucleotide molecule, and, optionally, translating the transcript into a polypeptide. An expression construct typically comprises in a 5' to 3' direction: [0140] a) a promoter functional in the host cell into which the construct will be transformed, [0141] b) the polynucleotide to be expressed, and [0142] c) a terminator functional in the host cell into which the construct will be transformed.

[0143] The term "coding region" or "open reading frame" (ORF) refers to the sense strand of a genomic DNA sequence or a cDNA sequence that is capable of producing a transcription product and/or a polypeptide under the control of appropriate regulatory sequences. The coding sequence is identified by the presence of a 5' translation start codon and a 3' translation stop codon. When inserted into a genetic construct, a "coding sequence" is capable of being expressed when it is operably linked to promoter and terminator sequences.

[0144] "Operably-linked" means that the sequenced to be expressed is placed under the control of regulatory elements that include promoters, tissue-specific regulatory elements, temporal regulatory elements, enhancers, repressors and terminators.

[0145] The term "noncoding region" refers to untranslated sequences that are upstream of the translational start site and downstream of the translational stop site. These sequences are also referred to respectively as the 5' UTR and the 3' UTR. These regions include elements required for transcription initiation and termination and for regulation of translation efficiency.

[0146] Terminators are sequences, which terminate transcription, and are found in the 3' untranslated ends of genes downstream of the translated sequence. Terminators are important determinants of mRNA stability and in some cases have been found to have spatial regulatory functions.

[0147] The term "promoter" refers to nontranscribed cis-regulatory elements upstream of the coding region that regulate gene transcription. Promoters comprise cis-initiator elements which specify the transcription initiation site and conserved boxes such as the TATA box, and motifs that are bound by transcription factors.

[0148] A "transgene" is a polynucleotide that is taken from one organism and introduced into a different organism by transformation. The transgene may be derived from the same species or from a different species as the species of the organism into which the transgene is introduced.

[0149] An "inverted repeat" is a sequence that is repeated, where the second half of the repeat is in the complementary strand, e.g.,

TABLE-US-00001 (5')GATCTA.......TAGATC(3') (3')CTAGAT.......ATCTAG(5')

[0150] Read-through transcription will produce a transcript that undergoes complementary base-pairing to form a hairpin structure provided that there is a 3-5 bp spacer between the repeated regions.

[0151] A "transgenic plant" refers to a plant which contains new genetic material as a result of genetic manipulation or transformation. The new genetic material may be derived from a plant of the same species as the resulting transgenic plant or from a different species.

[0152] The terms "to alter expression of" and "altered expression" of a polynucleotide or polypeptide of the invention, are intended to encompass the situation where genomic DNA corresponding to a polynucleotide of the invention is modified thus leading to altered expression of a polynucleotide or polypeptide of the invention. Modification of the genomic DNA may be through genetic transformation or other methods known in the art for inducing mutations. The "altered expression" can be related to an increase or decrease in the amount of messenger RNA and/or polypeptide produced and may also result in altered activity of a polypeptide due to alterations in the sequence of a polynucleotide and polypeptide produced.

[0153] The term "seed yield" refers to the size and/or mass and/or number of seed or grain produced by a plant. Thus a plant with increased seed yield has increased size and/or mass and/or number of seed or grain relative to a suitable control plant at the same age or an equivalent developmental stage. Conversely, a plant with decreased seed yield has increased size and/or mass and/or number of seed or grain relative to a suitable control plant the same age or an equivalent development stage.

[0154] Suitable control plants may include non-transformed plants of the same species and variety, or plants of the same species or variety transformed with a control construct.

[0155] The term "altered" with reference to seed yield is intended to encompass either a decrease or increase in seed yield.

[0156] The term "modulating" with reference to seed yield is intended to encompass either decreasing or increasing seed yield.

[0157] The invention provides methods for producing and/or selecting plants with altered seed yield relative to suitable control plants, including plants with both increased and decreased seed yield and plants produced by such methods.

[0158] The invention provides a polynucleotide (SEQ ID NO: 34) encoding a polypeptide (SEQ ID NO:1) which modulates seed yield in plants. The invention provides polynucleotide variants of SEQ ID NO:34 (SEQ ID NO: 35 to 65) which encode polypeptide variants of SEQ ID NO: 1 (SEQ ID NO:2 to 31). The applicants have also identified a consensus polypeptide sequence (SEQ ID NO: 32) present in SEQ ID NO: 1 to 31 and a second consensus polypeptide sequence (SEQ ID NO:33) present in the sequences from monocotyledonous plants selected from within SEQ ID NO:1 to 31.

Methods for Isolating or Producing Polynucleotides

[0159] The polynucleotide molecules of the invention can be isolated by using a variety of techniques known to those of ordinary skill in the art. By way of example, such polynucleotides can be isolated through use of the polymerase chain reaction (PCR) described in Mullis et al., Eds. 1994 The Polymerase Chain Reaction, Birkhauser, incorporated herein by reference. The polypeptides of the invention can be amplified using primers, as defined herein, derived from the polynucleotide sequences of the invention.

[0160] Further methods for isolating polynucleotides, of the invention or useful in the methods of the invention, include use of all, or portions of, the polynucleotides set forth herein as hybridization probes. The technique of hybridizing labeled polynucleotide probes to polynucleotides immobilized on solid supports such as nitrocellulose filters or nylon membranes, can be used to screen the genomic or cDNA libraries. Exemplary hybridization and wash conditions are: hybridization for 20 hours at 65° C. in 5.0×SSC, 0.5% sodium dodecyl sulfate, 1X Denhardt's solution; washing (three washes of twenty minutes each at 55° C.) in 1.0×SSC, 1% (w/v) sodium dodecyl sulfate, and optionally one wash (for twenty minutes) in 0.5 X SSC, 1% (w/v) sodium dodecyl sulfate, at 60° C. An optional further wash (for twenty minutes) can be conducted under conditions of 0.1×SSC, 1% (w/v) sodium dodecyl sulfate, at 60° C.

[0161] The polynucleotide fragments of the invention may be produced by techniques well-known in the art such as restriction endonuclease digestion and oligonucleotide synthesis.

[0162] A partial polynucleotide sequence may be used, in methods well-known in the art to identify the corresponding full length polynucleotide sequence. Such methods include PCR-based methods, 5'RACE (Frohman M A, 1993, Methods Enzymol. 218: 340-56) and hybridization-based method, computer/database-based methods. Further, by way of example, inverse PCR permits acquisition of unknown sequences, flanking the polynucleotide sequences disclosed herein, starting with primers based on a known region (Triglia et al., 1998, Nucleic Acids Res 16, 8186, incorporated herein by reference). The method uses several restriction enzymes to generate a suitable fragment in the known region of a gene. The fragment is then circularized by intramolecular ligation and used as a PCR template. Divergent primers are designed from the known region. In order to physically assemble full-length clones, standard molecular biology approaches can be utilized (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987).

[0163] It may be beneficial, when producing a transgenic plant from a particular species, to transform such a plant with a sequence or sequences derived from that species. The benefit may be to alleviate public concerns regarding cross-species transformation in generating transgenic organisms. Additionally when down-regulation of a gene is the desired result, it may be necessary to utilise a sequence identical (or at least highly similar) to that in the plant, for which reduced expression is desired. For these reasons among others, it is desirable to be able to identify and isolate orthologues of a particular gene in several different plant species. Variants (including orthologues) may be identified by the methods described.

Methods for Identifying Variants

Physical Methods

[0164] Variant polynucleotides may be identified using PCR-based methods (Mullis et al., Eds. 1994 The Polymerase Chain Reaction, Birkhauser). Typically, the polynucleotide sequence of a primer, useful to amplify variant polynucleotide molecules PCR, may be based on a sequence encoding a conserved region of the corresponding amino acid sequence.

[0165] Alternatively library screening methods, well known to those skilled in the art, may be employed (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987). When identifying variants of the probe sequence, hybridization and/or wash stringency will typically be reduced relatively to when exact sequence matches are sought.

[0166] Polypeptide variants may also be identified by physical methods, for example by screening expression libraries using antibodies raised against polypeptides of the invention (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987) or by identifying polypeptides from natural sources with the aid of such antibodies.

Computer Based Methods

[0167] Polynucleotide and polypeptide variants, may also be identified by computer-based methods well-known to those skilled in the art, using public domain sequence alignment algorithms and sequence similarity search tools to search sequence databases (public domain databases include Genbank, EMBL, Swiss-Prot, PIR and others). See, e.g., Nucleic Acids Res. 29: 1-10 and 11-16, 2001 for examples of online resources. Similarity searches retrieve and align target sequences for comparison with a sequence to be analyzed (i.e., a query sequence). Sequence comparison algorithms use scoring matrices to assign an overall score to each of the alignments.

[0168] An exemplary family of programs useful for identifying variants in sequence databases is the BLAST suite of programs (version 2.2.5 [Nov. 2002]) including BLASTN, BLASTP, BLASTX, tBLASTN and tBLASTX, which are publicly available from (ftp://ftp.ncbi.nih.gov/blast/) or from the National Center for Biotechnology Information (NCBI), National Library of Medicine, Building 38A, Room 8N805, Bethesda, Md. 20894 USA. The NCBI server also provides the facility to use the programs to screen a number of publicly available sequence databases. BLASTN compares a nucleotide query sequence against a nucleotide sequence database. BLASTP compares an amino acid query sequence against a protein sequence database. BLASTX compares a nucleotide query sequence translated in all reading frames against a protein sequence database. tBLASTN compares a protein query sequence against a nucleotide sequence database dynamically translated in all reading frames. tBLASTX compares the six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database. The BLAST programs may be used with default parameters or the parameters may be altered as required to refine the screen.

[0169] The use of the BLAST family of algorithms, including BLASTN, BLASTP, and BLASTX, is described in the publication of Altschul et al., Nucleic Acids Res. 25: 3389-3402, 1997.

[0170] The "hits" to one or more database sequences by a queried sequence produced by BLASTN, BLASTP, BLASTX, tBLASTN, tBLASTX, or a similar algorithm, align and identify similar portions of sequences. The hits are arranged in order of the degree of similarity and the length of sequence overlap. Hits to a database sequence generally represent an overlap over only a fraction of the sequence length of the queried sequence.

[0171] The BLASTN, BLASTP, BLASTX, tBLASTN and tBLASTX algorithms also produce "Expect" values for alignments. The Expect value (E) indicates the number of hits one can "expect" to see by chance when searching a database of the same size containing random contiguous sequences. The Expect value is used as a significance threshold for determining whether the hit to a database indicates true similarity. For example, an E value of 0.1 assigned to a polynucleotide hit is interpreted as meaning that in a database of the size of the database screened, one might expect to see 0.1 matches over the aligned portion of the sequence with a similar score simply by chance. For sequences having an E value of 0.01 or less over aligned and matched portions, the probability of finding a match by chance in that database is 1% or less using the BLASTN, BLASTP, BLASTX, tBLASTN or tBLASTX algorithm.

[0172] Multiple sequence alignments of a group of related sequences can be carried out with DbCLUSTAL (Thompson J. D., Plewnial F., Thierry J.-C. and Poch O. (2000) Rapid and reliable global multiple alignments of protein sequences detected by database searches. Nucleic Acid Research, Vol. 28, No 15: 2919-2926, http://www.ebi.ac.uk/cgi-bin/dbclustal/submit) or CLUSTALW (Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680, http://www-igbmc.u-strasbg.fr/BioInfo/ClustalW/Top.html) or T-COFFEE (Cedric Notredame, Desmond G. Higgins, Jaap Hering a, T-Coffee: A novel method for fast and accurate multiple sequence alignment, J. Mol. Biol. (2000) 302: 205-217)) or PILEUP, which uses progressive, pairwise alignments. (Feng and Doolittle, 1987, J. Mol. Evol. 25, 351).

[0173] Pattern recognition software applications are available for finding motifs or signature sequences. For example, MEME (Multiple Em for Motif Elicitation) finds motifs and signature sequences in a set of sequences, and MAST (Motif Alignment and Search Tool) uses these motifs to identify similar or the same motifs in query sequences. The MAST results are provided as a series of alignments with appropriate statistical data and a visual overview of the motifs found. MEME and MAST were developed at the University of California, San Diego.

[0174] PROSITE (Bairoch and Bucher, 1994, Nucleic Acids Res. 22, 3583; Hofmann et al., 1999, Nucleic Acids Res. 27, 215) is a method of identifying the functions of uncharacterized proteins translated from genomic or cDNA sequences. The PROSITE database (www.expasy.org/prosite) contains biologically significant patterns and profiles and is designed so that it can be used with appropriate computational tools to assign a new sequence to a known family of proteins or to determine which known domain(s) are present in the sequence (Falquet et al., 2002, Nucleic Acids Res. 30, 235). Prosearch is a tool that can search SWISS-PROT and EMBL databases with a given sequence pattern or signature.

Methods for Isolating Polypeptides

[0175] The polypeptides of the invention, including variant polypeptides, may be prepared using peptide synthesis methods well known in the art such as direct peptide synthesis using solid phase techniques (e.g. Stewart et al., 1969, in Solid-Phase Peptide Synthesis, WH Freeman Co, San Francisco Calif., or automated synthesis, for example using an Applied Biosystems 431A Peptide Synthesizer (Foster City, Calif.). Mutated forms of the polypeptides may also be produced during such syntheses.

[0176] The polypeptides and variant polypeptides of the invention may also be purified from natural sources using a variety of techniques that are well known in the art (e.g. Deutscher, 1990, Ed, Methods in Enzymology, Vol. 182, Guide to Protein Purification).

[0177] Alternatively the polypeptides and variant polypeptides of the invention may be expressed recombinantly in suitable host cells and separated from the cells as discussed below.

Methods for Producing Constructs and Vectors

[0178] The genetic constructs of the present invention comprise one or more polynucleotide sequences of the invention and/or polynucleotides encoding polypeptides of the invention, and may be useful for transforming, for example, bacterial, fungal, insect, mammalian or plant organisms. The genetic constructs of the invention are intended to include expression constructs as herein defined.

[0179] Methods for producing and using genetic constructs and vectors are well known in the art and are described generally in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing, 1987).

Methods for Producing Host Cells Comprising Constructs and Vectors

[0180] The invention provides a host cell which comprises a genetic construct or vector of the invention. Host cells may be derived from, for example, bacterial, fungal, insect, mammalian or plant organisms.

[0181] Host cells comprising genetic constructs, such as expression constructs, of the invention are useful in methods well known in the art (e.g. Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987; Ausubel et al., Current Protocols in Molecular Biology, Greene Publishing, 1987) for recombinant production of polypeptides of the invention. Such methods may involve the culture of host cells in an appropriate medium in conditions suitable for or conducive to expression of a polypeptide of the invention. The expressed recombinant polypeptide, which may optionally be secreted into the culture, may then be separated from the medium, host cells or culture medium by methods well known in the art (e.g. Deutscher, Ed, 1990, Methods in Enzymology, Vol 182, Guide to Protein Purification).

[0182] Host cells of the invention may also be useful in methods for production of an enzymatic product generated by an expressed polypeptide of the invention. Such methods may involve culturing the host cells of the invention in a medium suitable for expression of a recombinant polypeptide of the invention, optionally in the presence of additional enzymatic substrate for the expressed polypeptide of the invention. The enzymatic product produced may then be separated from the host cells or medium by a variety of art standard methods.

Methods for Producing Plant Cells and Plants Comprising Constructs and Vectors

[0183] The invention further provides plant cells which comprise a genetic construct of the invention, and plant cells modified to alter expression of a polynucleotide or polypeptide of the invention. Plants comprising such cells also form an aspect of the invention.

[0184] Production of plants altered in seed yield may be achieved through methods of the invention. Such methods may involve the transformation of plant cells and plants, with a construct designed to alter expression of a polynucleotide or polypeptide capable of modulating seed yield in such plant cells and plants. Such methods also include the transformation of plant cells and plants with a combination of constructs designed to alter expression of one or more polypeptides or polypeptides capable of modulating seed yield in such plant cells and plants.

[0185] Methods for transforming plant cells, plants and portions thereof with polynucleotides are described in Draper et al., 1988, Plant Genetic Transformation and Gene Expression. A Laboratory Manual, Blackwell Sci. Pub. Oxford, p. 365; Potrykus and Spangenburg, 1995, Gene Transfer to Plants. Springer-Verlag, Berlin; and Gelvin et al., 1993, Plant Molecular Biol. Manual. Kluwer Acad. Pub. Dordrecht. A review of transgenic plants, including transformation techniques, is provided in Galun and Breiman, 1997, Transgenic Plants. Imperial College Press, London.

Methods for Genetic Manipulation of Plants

[0186] A number of strategies for genetically manipulating plants are available (e.g. Birch, 1997, Ann Rev Plant Phys Plant Mol Biol, 48, 297). For example, strategies may be designed to increase expression of a polynucleotide/polypeptide in a plant cell, organ and/or at a particular developmental stage where/when it is normally expressed or to ectopically express a polynucleotide/polypeptide in a cell, tissue, organ and/or at. a particular developmental stage which/when it is not normally expressed. The expressed polynucleotide/polypeptide may be derived from the plant species to be transformed or may be derived from a different plant species.

[0187] Transformation strategies may be designed to reduce expression of a polynucleotide/polypeptide in a plant cell, tissue, organ or at a particular developmental stage which/when it is normally expressed. Such strategies are known as gene silencing strategies.

[0188] Genetic constructs for expression of genes in transgenic plants typically include promoters for driving the expression of one or more cloned polynucleotide, terminators and selectable marker sequences to detest presence of the genetic construct in the transformed plant.

[0189] The promoters suitable for use in the constructs of this invention are functional in a cell, tissue or organ of a monocot or dicot plant and include cell-, tissue- and organ-specific promoters, cell cycle specific promoters, temporal promoters, inducible promoters, constitutive promoters that are active in most plant tissues, and recombinant promoters. Choice of promoter will depend upon the temporal and spatial expression of the cloned polynucleotide, so desired. The promoters may be those normally associated with a transgene of interest, or promoters which are derived from genes of other plants, viruses, and plant pathogenic bacteria and fungi. Those skilled in the art will, without undue experimentation, be able to select promoters that are suitable for use in modifying and modulating plant traits using genetic constructs comprising the polynucleotide sequences of the invention. Examples of constitutive plant promoters include the CaMV 35S promoter, the nopaline synthase promoter and the octopine synthase promoter, and the Ubi 1 promoter from maize. Plant promoters which are active in specific tissues, respond to internal developmental signals or external abiotic or biotic stresses are described in the scientific literature. Exemplary promoters are described, e.g., in WO 02/00894, which is herein incorporated by reference.

[0190] Exemplary terminators that are commonly used in plant transformation genetic construct include, e.g., the cauliflower mosaic virus (CaMV) 35S terminator, the Agrobacterium tumefaciens nopaline synthase or octopine synthase terminators, the Zea mays zin gene terminator, the Oryza sativa ADP-glucose pyrophosphorylase terminator and the Solanum tuberosum PI-II terminator.

[0191] Selectable markers commonly used in plant transformation include the neomycin phophotransferase II gene (NPT II) which confers kanamycin resistance, the aadA gene, which confers spectinomycin and streptomycin resistance, the phosphinothricin acetyl transferase (bar gene) for Ignite (AgrEvo) and Basta (Hoechst) resistance, and the hygromycin phosphotransferase gene (hpt) for hygromycin resistance.

[0192] Use of genetic constructs comprising reporter genes (coding sequences which express an activity that is foreign to the host, usually an enzymatic activity and/or a visible signal (e.g., luciferase, GUS, GFP) which may be used for promoter expression analysis in plants and plant tissues are also contemplated. The reporter gene literature is reviewed in Herrera-Estrella et al., 1993, Nature 303, 209, and Schrott, 1995, In: Gene Transfer to Plants (Potrykus, T., Spangenbert. Eds) Springer Verlag. Berline, pp. 325-336.

[0193] Gene silencing strategies may be focused on the gene itself or regulatory elements which effect expression of the encoded polypeptide. "Regulatory elements" is used here in the widest possible sense and includes other genes which interact with the gene of interest.

[0194] Genetic constructs designed to decrease or silence the expression of a polynucleotide/polypeptide of the invention may include an antisense copy of a polynucleotide of the invention. In such constructs the polynucleotide is placed in an antisense orientation with respect to the promoter and terminator.

[0195] An "antisense" polynucleotide is obtained by inverting a polynucleotide or a segment of the polynucleotide so that the transcript produced will be complementary to the mRNA transcript of the gene, e.g.,

TABLE-US-00002 5'GATCTA 3' 3'CTAGAT 5' (coding strand) (antisense strand) 3'CUAGAU 5' 5'GAUCUCG 3' mRNA antisense RNA

[0196] Genetic constructs designed for gene silencing may also include an inverted repeat. An `inverted repeat` is a sequence that is repeated where the second half of the repeat is in the complementary strand, e.g.,

TABLE-US-00003 5'-GATCTA.........TAGATC-3' 3'-CTAGAT.........ATCTAG-5'

[0197] The transcript formed may undergo complementary base pairing to form a hairpin structure. Usually a spacer of at least 3-5 bp between the repeated region is required to allow hairpin formation.

[0198] Another silencing approach involves the use of a small antisense RNA targeted to the transcript equivalent to an miRNA (Llave et al., 2002, Science 297, 2053). Use of such small antisense RNA corresponding to polynucleotide of the invention is expressly contemplated.

[0199] The term genetic construct as used herein also includes small antisense RNAs and other such polypeptides effecting gene silencing.

[0200] Transformation with an expression construct, as herein defined, may also result in gene silencing through a process known as sense suppression (e.g. Napoli et al., 1990, Plant Cell 2, 279; de Carvalho Niebel et al., 1995, Plant Cell, 7, 347). In some cases sense suppression may involve over-expression of the whole or a partial coding sequence but may also involve expression of non-coding region of the gene, such as an intron or a 5' or 3' untranslated region (UTR). Chimeric partial sense constructs can be used to coordinately silence multiple genes (Abbott et al., 2002, Plant Physiol. 128(3): 844-53; Jones et al., 1998, Planta 204: 499-505). The use of such sense suppression strategies to silence the expression of a polynucleotide of the invention is also contemplated.

[0201] The polynucleotide inserts in genetic constructs designed for gene silencing may correspond to coding sequence and/or non-coding sequence, such as promoter and/or intron and/or 5' or 3' UTR sequence, or the corresponding gene.

[0202] Other gene silencing strategies include dominant negative approaches and the use of ribozyme constructs (McIntyre, 1996, Transgenic Res, 5, 257)

[0203] Pre-transcriptional silencing may be brought about through mutation of the gene itself or its regulatory elements. Such mutations may include point mutations, frameshifts, insertions, deletions and substitutions.

[0204] The following are representative publications disclosing genetic transformation protocols that can be used to genetically transform the following plant species: Rice (Alam et al., 1999, Plant Cell Rep. 18, 572); maize (U.S. Pat. Nos. 5,177,010 and 5,981,840); wheat (Ortiz et al., 1996, Plant Cell Rep. 15, 1996, 877); tomato (U.S. Pat. No. 5,159,135); potato (Kumar et al., 1996 Plant J. 9: 821); cassaya (Li et al., 1996 Nat. Biotechnology 14, 736); lettuce (Michelmore et al., 1987, Plant Cell Rep. 6, 439); tobacco (Horsch et al., 1985, Science 227, 1229); cotton (U.S. Pat. Nos. 5,846,797 and 5,004,863); grasses (U.S. Pat. Nos. 5,187,073, 6,020,539); peppermint (Niu et al., 1998, Plant Cell Rep. 17, 165); citrus plants (Pena et al., 1995; Plant Sci. 104, 183); caraway (Krens et al., 1997, Plant Cell Rep, 17, 39); banana (U.S. Pat. No. 5,792,935); soybean (U.S. Pat. Nos. 5,416,011; 5,569,834; 5,824,877; 5,563,044,55 and 5,968,830); pineapple (U.S. Pat. No. 5,952,543); poplar (U.S. Pat. No. 4,795,855); monocots in general (U.S. Pat. Nos. 5,591,616 and 6,037,522); brassica (U.S. Pat. Nos. 5,188,985; 5,463,174 and 5,750,871); and cereals (U.S. Pat. No. 6,074,877). Other species are contemplated and suitable methods and protocols are available to in the scientific literature for use by those skilled in the art.

[0205] Several further methods known in the art may be employed to alter expression of a nucleotide and/or polypeptide of the invention. Such methods include but are not limited to Tilling (Till et al., 2003, Methods Mol Biol, 2%, 205), so called "Deletagene" technology (Li et al., 2001, Plant Journal 27(3), 235) and the use of artificial transcription factors such as synthetic zinc finger transcription factors. (e.g. Jouvenot et al., 2003, Gene Therapy 10, 513). Additionally antibodies or fragments thereof, targeted to a particular polypeptide may also be expressed in plants to modulate the activity of that polypeptide (Jobling et al., 2003, Nat. Biotechnol., 21(1), 35). Transposon tagging approaches may also be applied. Additionally peptides interacting with a polypeptide of the invention may be identified through technologies such as phase-display (Dyax Corporation). Such interacting peptides may be expressed in or applied to a plant to affect activity of a polypeptide of the invention. Use of each of the above approaches in alteration of expression of a nucleotide and/or polypeptide of the invention is specifically contemplated.

Methods for Selecting Plants

[0206] Methods are also provided for selecting plants with altered seed yield. Such methods involve testing of plants for altered for the expression of a polynucleotide or polypeptide of the invention. Such methods may be applied at a young age or early developmental stage when the altered seed yield may not necessarily be visible, to accelerate breeding programs directed toward improving seed yield.

[0207] The expression of a polynucleotide, such as a messenger RNA, is often used as an indicator of expression of a corresponding polypeptide. Exemplary methods for measuring the expression of a polynucleotide include but are not limited to Northern analysis, RT-PCR and dot-blot analysis (Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Press, 1987). Polynucleotides or portions of the polynucleotides of the invention are thus useful as probes or primers, as herein defined, in methods for the identification of plants with altered seed yield. The polypeptides of the invention may be used as probes in hybridization experiments, or as primers in PCR based experiments, designed to identify such plants.

[0208] Alternatively antibodies may be raised against polypeptides of the invention. Methods for raising and using antibodies are standard in the art (see for example: Antibodies, A Laboratory Manual, Harlow A Lane, Eds, Cold Spring Harbour Laboratory, 1998). Such antibodies may be used in methods to detect altered expression of polypeptides which modulate seed yield in plants. Such methods may include ELISA (Kemeny, 1991, A Practical Guide to ELISA, NY Pergamon Press) and Western analysis (Towbin & Gordon, 1994, J Immunol Methods, 72, 313).

[0209] These approaches for analysis of polynucleotide or polypeptide expression and the selection of plants with altered expression are useful in conventional breeding programs designed to produce varieties with altered seed yield.

Plants

[0210] The plants of the invention may be grown and either self-ed or crossed with a different plant strain and the resulting hybrids, with the desired phenotypic characteristics, may be identified. Two or more generations may be grown to ensure that the subject phenotypic characteristics are stably maintained and inherited. Plants resulting from such standard breeding approaches also form an aspect of the present invention.

[0211] Seed yield in a plant may also be altered through methods of the invention. Such methods may involve the transformation of plant cells and plants, with a construct of the invention designed to alter expression of a polynucleotide or polypeptide which modulates seed yield in such plant cells and plants. Such methods also include the transformation of plant cells and plants with a combination of the construct of the invention and one or more other constructs designed to alter expression of one or more polynucleotides or polypeptides which modulates seed yield in plants.

[0212] Exemplary methods for assessing seed yield in plants are provided in Boyes D C et al., 2001, Plant Cell. 13(7):1499-510; Lancashire P. D et al., 1991, Ann. Appl. Biol. 119:560-601, and in Example 1 below.

[0213] This invention may also be said broadly to consist in the parts, elements and features referred to or indicated in the specification of the application, individually or collectively, and any or all combinations of any two or more said parts, elements or features, and where specific integers are mentioned herein which have known equivalents in the art to which this invention relates, such known equivalents are deemed to be incorporated herein as if individually set forth.

BRIEF DESCRIPTION OF THE DRAWINGS

[0214] The present invention will be better understood with reference to the accompanying drawings in which:

[0215] FIG. 1 shows the output summary of a BLASTP search of the uniref100 database (version 2.0 MP-WashU [4 May 2006]) in which the ORF56 polypeptide was used as a seed sequence.

[0216] FIG. 2 shows a "Prettyplot" alignment of polypeptides (SEQ ID NO: 1 to 31) including ORF56 and variants thereof and illustrates a consensus region identified by the applicants which is present in all of the sequences.

[0217] FIG. 3 shows a "Prettyplot" alignment of polypeptides of the invention, including ORF56 SEQ ID NO:1 and sequences from all variants of SEQ ID NO:1 and illustrates a consensus GAD region identified by the applicants which is present in all of the such sequences.

[0218] FIG. 4 shows another "Prettyplot" alignment of polypeptides of the invention, including ORF 56 SEQ ID NO: 1 and sequence variants SEQ ID NO:2-31 and illustrates a consensus CaM region identified by the applicants which is present in all of the such sequences.

[0219] FIG. 5 shows a map of an over-expression vector, for plant transformation, comprising ORF56 cloned in sense orientation (SEQ ID NO:66).

[0220] FIG. 6 shows a DNA gel-blot analysis on genomic DNA from ORF56 T0 transgenic plants digested with a restriction enzyme and probed with a fragment of ORF56 coding sequence to determine gene copy number and to identify independent transformation events.

[0221] FIGS. 7a-7d show the growth parameters observed for transgenic ORF56 T1 plant lines compared to the best performing wild type control (Nipponbare). Where FIG. 7a. Plant height measurements from experiment 1, FIG. 7b. Plant tiller measurements from experiment 1, FIG. 7c. Plant height measurements from experiment 2, FIG. 7d. Plant tiller measurements from experiment 1.

[0222] FIGS. 8a-8e show the seed and grain characteristics for transgenic ORF56 T1 plant lines compared to the best performing wild type control (Nipponbare). Where FIG. 8a, Seed yield per plant in different ORF56 T1 lines, FIG. 8b Total number of seeds per plant in different ORF56 T1 lines, FIG. 8c Average mass of an individual seed in each ORF56 T1 line, FIG. 8d Binomial distribution for seed yields in different ORF56 T1 lines, FIG. 8e Shift in seed yield distribution in the different ORF56 T1 lines.

[0223] FIG. 9 shows the seeds/grain harvested from the best yielding control plant (Nipponbare) and ORF56 T1 plant.

[0224] FIG. 10 shows an alignment of the ORF56 polypeptide sequence (SEQ ID NO: 1) and all of the other variants sequences disclosed that are from monocotyledonous species. The position of a completely conserved motif within the GAD region is shown. The sequence of the completely conserved motif is shown in SEQ ID NO: 32.

[0225] FIG. 11 shows an alignment of the ORF56 polypeptide sequence (SEQ ID NO: 1) and all of the other variant sequences disclosed that are from dicotyledonous species. The position of a completely conserved motif within the GAD region is shown. This sequence motif is completely conserved in all of the dicotyledonous sequences and all of the monocotyledonous sequences (see FIG. 10) and is shown on SEQ ID NO: 33.

EXAMPLES

[0226] The invention will now be illustrated with reference to the following non-limiting examples.

Example 1

Altered Seed Yield by in-Plant Expression of a Polynucleotide of the Invention

ORF56

[0227] A polynucleotide designated ORF56 (SEQ ID NO:34) was identified in a ViaLactia Biosciences Ltd proprietary ryegrass (Lolium perenne) GeneThresher (Orion Genomics) genomic library. ORF56 appears to encode a polypeptide (SEQ ID NO:1) which is a glutamate decarboxylase (GAD). GAD catalyses the reaction of glutamate into carbon dioxide and gamma-aminobutyrate (GABA). GAD is believed to be essential for cytosolic pH regulation as well as basic morphological development. The synthesis of GABA is highly regulated under normal growth conditions and in response to stresses such as cold, heat, water or mechanical (Bouche et al 2004; Mayer et al 1990: Patterson and Graham 1987; Wallace et al. 1984). It has been shown that GABA stimulates ethylene production in sunflower, apparently by causing increases in ACC synthase mRNA accumulation, ACC levels, ACC oxidase mRNA levels and ACC oxidase activity, suggesting that GABA may play a role in signaling (Kathiresan et al 1997). High GABA has been reported to impair pollen tube growth, and consequently seed set, while lower levels are reported to be stimulatory (Updegraff and Preuss 2004). The glutamate decarboxylase encoded by ORF56 contains two major domains, the GAD catalytic domain and a second Calmodulin binding domain (CaM). CaM has been shown to stimulate GAD activity by binding to a C-terminal `extension` not found in prokaryotes such as E. coli or mammalian forms of GAD. Truncation of this domain results in shorter, more branched plants with delayed greening and lacking pollen (Baum et al 1996; Akama and Takaiwa 2007). Post-translational modifications of GAD may account for different phenotypes observed. GAD is a monomer in aqueous solutions. Normally, the binding of a Trp residue in the hydrophobic region of the peptide to the C-terminal domain of CaM (boxed residue in FIG. 4) initiates the peptide complex formation. Subsequently, the N-terminal lobe of CaM binds to the hydrophobic and positively charged residues of the region of the peptide 10-12 residues away from the Trp residue (Yuan and Vogel 1998). However, in perennial ryegrass, SEQ ID: 1, the CaM binding domain, lacks the hydrophobic residue Trp, but instead is replaced with a polar but non-charged Cys residue in a hydrophobic setting. The negative charges in perennial ryegrass CaM domain are also disposed in such a way that interaction of the N-terminal lobe of CaM with the region either N- or C-terminal to the GAD would not be energetically favourable, thereby facilitating binding of a second or multiple GAD peptides to the hydrophobic surface in the N-terminal region of CaM. This essentially results in the dimerization or multimerization of the GAD-Ca²+-CaM complex. There are possibilities for the GAD such dimerization, which seems to be induced by Ca²+-CaM binding (Yuan and Vogel 1998). In plants, there is evidence for the oligomerization of petunia GAD and it appears that only oligomerized GAD seem to be active in petunia (Baum et al 1996). In addition, various isoforms of active GAD extracted from barley were reported to have a mass of ˜256 kDa and ˜120 kDa (Inatomi and Slaughter 1975). The activity of the barley GADs were also reported to exhibit a reversible inhibition in the presence of 2-Mercaptoethanol. Given these evidences, it is not unreasonable to expect the Cys residue, at the normally Trp residue position in the CaM domain, to play a role in stabilizing the dimerized GAD-Ca²+-CaM complex.

ORF56 Variants

[0228] The polypeptide sequence encoded by the ORF56 was used as seed sequence to perform WU-blastp search against uniref100 database (2.0 MP-WashU [4 May 2006]) to identify variants of ORF56. The WU-blastp output summary is shown in FIG. 1. A cut-off e value of less than or equal to 5.5e-169, was identified as distinguishing variants of ORF56 from unrelated proteins, based upon assessment of the associated score value and annotations in the public database. Selected variant sequences were aligned using the DbCLUSTAL (Thompson J. D., Plewnial F., Thierry J.-C. and Poch O.: 2000), which is an interface to the popular multiple alignment program ClustalW. Aligned sequences were visualised using another EMBOSS tool called Prettyplot as shown in FIG. 2.

[0229] The variant polypeptide sequences of ORF56 are listed as SEQ ID NO:2-31 in the sequence listing. The corresponding polynucleotide sequences are listed as SEQ ID NO: 35-65.

[0230] All but four of the variant polypeptide sequences appear to have both a proper GAD catalytic domain and a proper CaM binding domain. Four of the sequences (polypeptide SEQ ID NO: 11, 15, 22 and 29; polynucleotide SEQ ID NO:61 to 66) appear to have a proper GAD catalytic domain with a variant CaM binding domain.

[0231] A conserved GAD polypeptide sequence region present in ORF56 and all of the identified variants of ORF 56 was identified using Prettyplot alignment as shown in FIG. 3.

[0232] A further conserved CaM polypeptide sequence region present in polypeptide sequences SEQ ID NO: 1-31 was also identified using Prettyplot alignment as shown in FIG. 4.

[0233] A completely conserved polypeptide sequence motif was identified in the GAD region in all of the variant sequences, and is shown on SEQ ID NO: 32.

[0234] A completely conserved polypeptide sequence motif was also identified in the GAD region in all of the variant sequences that are from monocotyledonous species and is shown in SEQ ID NO: 33.

Construction of a Vector for Over-Expression of ORF56 Via Plant Transformation

[0235] A vector for over-expression ORF56 was produced by standard molecular biology techniques. A map of the binary vector is shown in FIG. 5. The sequence of the vector is shown in SEQ ID NO: 66.

Plant Transformation

[0236] Agrobacterium tumefaciens strains can be transformed with binary plasmid DNA using either a freeze/thaw (Chen et. al 1994) or electroporation method (den Dulk-Ras A and Hooykaas P J.). Purified plasmid DNA of ORF56 was introduced into Agrobacterium strain EHA105 by electroporation and the suspension was incubated at 26° C. for 30 minutes. A small aliquot was plated on AB minimal medium (Schmidt-Eisenlohr et. al 1999) containing Kanamycin at 100 mg/L. Plates were incubated at 26° C. for 3 days and single colonies were tested for presence of the plasmid using construct specific primers and transformation confirmed.

[0237] Agrobacterium cultures were grown in AG minimal medium containing 100 mg/L kanamycin at 26° C. with shaking (200 rpm). The Agrobacterium suspensions were pelleted at 5,000 rpm for 5 minutes, washed once in basal MS medium containing 1% glucose and 3% sucrose, pH 5.2, and re-suspended in same medium containing 200 μM acetosyringone to OD₆₀₀ 0.6-0.8.

[0238] A. tumefaciens containing the binary vector ORF56 were used to co-cultivate at least 1,000 immature rice (Oryza sativa) cv. Nipponbare embryos. Immature seeds from rice were washed in sterile water and then surface sterilized with sodium hypochlorite containing 1.25% active chlorine with 10 μl, Tween® 20 for 20 minutes. After sterilization, the seeds were washed several times with sterile water and blotted dry on sterile filter paper (3M). The seeds were de-husked manually using sterile pair of forceps and the embryo dissected out with sterile knife. The isolated embryos were immersed in Agrobacterium suspension for 30 minutes with continuous shaking at 100 rpm in a 10 mL culture tube. The excess liquid was drained off and the embryos blotted on to sterile filter paper before placing them on to co-cultivation medium containing MS medium (Murashige and Skoog, 1964) supplemented with 3% sucrose, 1% glucose, 2 mg/L 2,4-D, 0.1 mg/L BA, 400 μM acetosyringone, pH 5.2 for 4 days in dark. After co-cultivation, the calli forming embryos were sub-cultured once every two weeks on selection medium consisting of MS medium supplemented with 3% sucrose, 1% glucose, 2 mg/L 2,4-D (2,4-dichlorophenoxy acetic acid), 0.1 mg/L BA (benzyl adenine) and containing 50 mg/L hygromycin and 300 mg/L Timentin® (ticarcillin+clavulanic acid) till at-least 30 healthy calli showing green spots indicative of healthy shoot emergence was achieved. Calli containing the green spots were transferred to selection medium lacking 2,4-D to regenerate a minimum of 10 transformed plants. Regenerated plants were rooted and then transplanted to six inch pots containing soil and plants grown in greenhouse. DNA gel-blot analysis was carried out (FIG. 6) to determine gene copy number and identify five independent transformation events. T1 seeds were harvested from the transformed plants (T0).

T1 Plant Phenotyping

[0239] Thirty seeds from Southern positive T0 plants were sown in individual cups containing cocopeat and twenty healthy plants out of them were transplanted in the green house. These plants were arranged using a CRD using the random numbers from a random table.

[0240] T1 plant phenotyping was carried out in two separate experiments. The first experiment involved progeny lines from T0 events 1129105 and 1129106 and Nipponbare (a wild-type control), and the second experiment involved progeny lines from T0 events 1129102, 1129103 and 112904 and Nipponbare (a wild-type control.)

Phenotypic Analysis of T0 Lines

[0241] The physiological state of T0 plants is presented in Table 1, below.

TABLE-US-00004 TABLE 1 Physiological measurements of T0 lines Pollen Productive Tillers/ Plant Seed T0 line fertility Total Number of Tillers height (cms) yield 1129102 72.2% 9/9 64.3 30 1129103 91.5% 14/15 69.6 30 1129104 79.7% 9/9 65.6 50 1129105 85.6% 8/8 84.2 130 1129106 81.5% 7/7 75.5 160

Phenotypic Analysis of T1 Lines

[0242] Plants height and tiller numbers were measured once every week post-transplanting until seed set was achieved. FIGS. 7a, b, c and d depict the growth parameters observed for these plants. Transgenic ORF56 plants (T1) were not different in terms of plant height and tillering capacity based upon standard statistical analysis. Transgenic ORF56 plants can be said to be normal in all aspects assessed (data not shown) except for seed yield, which was found in one of the plant progenies analysed to be as high as 3.55 times the normal seed yield.

[0243] FIG. 8a, b, c, d, and e describe the seed yield per plant, total number of seeds per plant, average mass of an individual seed in each line, binomial distribution for seed yields in different lines and shift in seed yield distribution in the lines analysed. It is evident from this analysis that the increase in seed yield is the result of an increase in seed number rather than an increase in individual seed weight (FIG. 8a, b and c). Given that the analysis was carried out using the segregating population in T1, it is not surprising that a shift is observed in the binomial distribution for seed yield trait in the transgenic populations investigated (FIGS. 8d and e). Conventional breeding techniques, such as single seed descent, can fix the trait for enhanced seed yield.

[0244] The seed yield from the best seed yielding plant from wild-type Nipponbare and the T10RF56 plant is presented in FIG. 9.

REFERENCES

[0245] Adams et al. 1991, Science 252:1651-1656. [0246] Akama, K and Takaiwa (2007) J Exp Bot.: 58 (10) 2699-2707. [0247] Baum G, Lev-Yadun S, Fridmann Y, Arazi T, Katsnelson H, Zik M, and Fromm H (1996) EMBO J.: 15 (12) 2988-2996. [0248] Bouche N, Fait A, Zik M, Fromm H. (2004) Plant Mol. Biol.; 55 (3): 315-25. [0249] Chen H, Nelson R S, Sherwood J L. (1994) Biotechniques; 16 (4): 664-8, 670. [0250] Chen et al. 2002, Nucleic Acids Res. 31:101-105 [0251] den Dulk-Ras A, Hooykaas P J. (1995) Methods Mol. Biol.; 55: 63-72. [0252] Inatomi, K and Slaughter, J C (1975) Biochem J. 147: 479-484. [0253] Kathiresan A, Tung P, Chinnappa C C, Reid D M (1997) Plant Physiol. 115: 129-135. [0254] Lee et al. 2003, PNAS 99:12257-12262 [0255] Lee and Lee, 2003 Plant Physiol. 132: 517-529 [0256] Mayer R R, Cherry J H, Rhodes D (1990) Plant Physiol. 94: 796-810. [0257] Murashige T, Skoog F (1962) Physiol Plant 15: 473-497 [0258] Patterson B D, Graham D (1987) In (DD Davies ed) "The Biochemistry of Plants", Vol 12, Academic Press, New York, pp. 153-199. [0259] Richmond and Somerville 2000, Current Opinion in Plant Biology. 3:108-116 [0260] Ruan et al. 2004, Trends in Biotechnology 22: 23-30. [0261] Schmidt-Eisenlohr H, Domke N, Angerer C, Wanner G, Zambryski P C, Baron C. (1999) J. Bacteriol.; 181 (24): 7485-92. [0262] Sun et al. 2004, BMC Genomics 5: 1.1-1.4 [0263] Thompson, J. D., Higgins, D. G. and Gibson, T. J. (1994) CABIOS, 10, 19-29. [0264] Updegraff E, Preuss D (2004) in International Arabidopsis Conference, Germany [0265] Velculescu et al. 1995, Science 270: 484-487 [0266] Wallace W, Secor J, Schrader L E (1984) Plant Physiol. 75: 170-175. [0267] Yuan, T and Vogel, H J (1998) J. Biol. Chem. 273 (46) 30328-30335.

[0268] The above examples illustrate practice of the invention. It will be appreciated by those skilled in the art that numerous variations and modifications may be made without departing from the spirit and scope of the invention.

SUMMARY OF SEQUENCES

TABLE-US-00005 [0269] SEQ ID NO: Sequence type Information Species 1 polypeptide ORF 56 Lolium perenne 2 polypeptide UniRef100_Q42521 Arabidopsis thaliana Glutamate decarboxylase 1 3 polypeptide UniRef100_Q42472 Arabidopsis thaliana Glutamate decarboxylase 2 4 polypeptide UniRef100_Q07346 Petunia hybrida Glutamate decarboxylase 5 polypeptide UniRef100_P54767 Lycopersicon esculentum Glutamate decarboxylase 6 polypeptide UniRef100_A0EJ88 Populus tremula × Glutamate decarboxylase Populus alba 7 polypeptide UniRef100_A2XEB3 Oryza sativa subsp. Putative uncharacterized indica protein 8 polypeptide UniRef100_A3AM59 Oryza sativa subsp. Putative uncharacterized japonica protein 9 polypeptide UniRef100_A5BI27 Vitis vinifera Putative uncharacterized protein 10 polypeptide UniRef100_A7P433 Vitis vinifera Chromosome chr1 scaffold_5, whole genome shotgun sequence 11 polypeptide UniRef100_A7P434 Vitis vinifera Chromosome chr1 scaffold_5, whole genome shotgun sequence 12 polypeptide UniRef100_O81101 Nicotiana tabacum Glutamate decarboxylase isozyme 2 13 polypeptide UniRef100_O81102 Nicotiana tabacum Glutamate decarboxylase isozyme 1 14 polypeptide UniRef100_P93369 Nicotiana tabacum Glutamate decarboxylase 15 polypeptide UniRef100_Q01J81 Oryza sativa OSIGBa0152K17.6 16 polypeptide UniRef100_Q1I1D8 Citrus sinensis Glutamate decarboxylase 17 polypeptide UniRef100_Q6ASV4 Oryza sativa subsp. Putative glutamate japonica decarboxylase isozyme 18 polypeptide UniRef100_Q6Q4I1 Brassica juncea Glutamate decarboxylase 4b 19 polypeptide UniRef100_Q6Q4I2 Brassica juncea Glutamate decarboxylase 4a 20 polypeptide UniRef100_Q6Q4I3 Brassica juncea Glutamate decarboxylase 2 21 polypeptide UniRef100_Q6YSB2 Oryza sativa subsp. japonica 22 polypeptide UniRef100_Q7XZU7 Hordeum vulgare GAD1 23 polypeptide UniRef100_Q84U04 Oryza sativa subsp. Glutamate decarboxylase japonica 24 polypeptide UniRef100_Q8LFR4 Arabidopsis thaliana Glutamate decarboxylase, putative 25 polypeptide UniRef100_Q8LKR4 Nicotiana tabacum Glutamate decarboxylase 26 polypeptide UniRef100_Q944L6 Arabidopsis thaliana At1g65960/F12P19_12 27 polypeptide UniRef100_Q94KK8 Nicotiana tabacum Glutamate decarboxylase isozyme 3 28 polypeptide UniRef100_Q9AR41 Oryza sativa subsp. Glutamate decaroxylase japonica 29 polypeptide UniRef100_Q9AR41 Oryza sativa subsp. Glutamate decarboxylase japonica 30 polypeptide UniRef100_Q9AT17 Nicotiana tabacum Glutamate decarboxylase isozyme 1 31 polypeptide UniRef100_Q9LSH2 Arabidopsis thaliana Glutamate dearboxylase 32 polypeptide GAD plant consensus Artificial 33 polypeptide CaM monocot plant Artificial consensus 34 polynucleotide ORF 56 Lolium perenne 35 polynucleotide XP_462650.10 Oryza sativa 36 polynucleotide AAN46801.1 Arabidopsis thaliana 37 polynucleotide XP_482841.1 Oryza sativa 38 polynucleotide AAS79671.1 Brassica juncea 39 polynucleotide AAS79670.1 Brassica juncea 40 polynucleotide AAS79669.1 Brassica juncea 41 polynucleotide BAB02870.1 Arabidopsis thaliana 42 polynucleotide AAM70569.1 Arabidopsis thaliana 43 polynucleotide BAC42751.1 Arabidopsis thaliana 44 polynucleotide AAK38667.1 | AF353615_1 Nicotiana tabacum 45 polynucleotide AAK18620.1 | AF352732_1 Nicotiana tabacum 46 polynucleotide AAP85548.1 Glycine Max 47 polynucleotide AAM48129.1 | AF506366_1 Nicotiana tabacum 48 polynucleotide AAM61251.1 Arabidopsis thaliana 49 polynucleotide AAP79441.1 Oryza sativa 50 polynucleotide AAL91148.1 Arabidopsis thaliana 51 polynucleotide XP_482840.1 Oryza sativa 52 polynucleotide AAL16126.1 | AF428294_1 Arabidopsis thaliana 53 polynucleotide AAC39483.1 Nicotiana tabacum 54 polynucleotide AAM70582.1 Arabidopsis thaliana 55 polynucleotide AAC24195.1 Nicotiana tabacum 56 polynucleotide AAB40608.1 Nicotiana tabacum 57 polynucleotide AAA93132.1 Arabidopsis thaliana 58 polynucleotide A48767 Petunia hybrida 59 polynucleotide AAL83983.1 Oryza sativa 60 polynucleotide CAA56812.1 Lycopersicon esculentum 61 polynucleotide XP_462654.1 Oryza sativa 62 polynucleotide AAM7304.1 | AF377946_6 Oryza sativa 63 polynucleotide BAB32871.1 Oryza sativa 64 polynucleotide AAT77842.1 Oryza sativa 65 polynucleotide AAP46640.1 Hordeum vulgare 66 polynucleotide vector Artificial 67 polynucleotide U10034.1 | codes AAA93132 Arabidopsis thaliana and UniRef100_Q42521 68 polynucleotide U46665.1 | codes AAC33485 Arabidopsis thaliana and UniRef100_Q42472 69 polynucleotide L16797.1 codes AAA33709 Petunia × hybrida and UniRef100_Q07346 70 polynucleotide X80840.1 | codes CAA56812 Solanum lycopersicum and UniRef100_P54767 71 polynucleotide DQ125945.1 | codes Populus tremula × Populus alba ABA18652 and UniRef100_A0EJ88 72 polynucleotide CM000128.1 | codes Oryza sativa Indica EAY89173 and UniRef100_A2XEB3 73 polynucleotide CM000140.1 | codes Oryza sativa Japonica EAZ28398 and UniRef100_A3AM59 74 polynucleotide AM460203.2 | codes Vitis vinifera CAN67952 and UniRef100_A5BI27 75 polynucleotide CU459222.1 | codes Vitis vinifera CAO42441 and UniRef100_A7P433 76 polynucleotide CU459222.1 | codes Vitis vinifera CAO42442 and UniRef100_A7P434 77 polynucleotide AF020424.1 | codes Nicotiana tabacum AAC39483 and UniRef100_O81101 78 polynucleotide AF020425.1 | codes Nicotiana tabacum AAC24195 and UniRef100_O81102 79 polynucleotide U54774.1 | codes AAB40608 Nicotiana tabacum and UniRef100_P93369 80 polynucleotide CR855179.1 | codes Oryza sativa CAH67194 and UniRef100_Q01J81 81 polynucleotide DQ001727.1 | codes Citrus sinensis AAZ05070 and UniRef100_Q1I1D8 82 polynucleotide DP000009.2 | codes Oryza sativa Japonica ABF98584 and UniRef100_Q6ASV4 83 polynucleotide AY559320.1 | codes Brassica juncea AAS79671 and UniRef100_Q6Q4I1 84 polynucleotide AY559319.1 | codes Brassica juncea AAS79670 and UniRef100_Q6Q4I2 85 polynucleotide AY559318.1 | codes Brassica juncea AAS79669 and UniRef100_Q6Q4I3 86 polynucleotide AP006461.3 | codes Oryza sativa Japonica BAD10771 and UniRef100_Q6YSB2 87 polynucleotide AF521177.1 | codes Hordeum vulgare AAP46640 and UniRef100_Q7XZU7 88 polynucleotide AY318775.1 | codes Oryza sativa Japonica AAP79441 and UniRef100_Q84U04 89 polynucleotide AY084689.1 | codes Arabidopsis thaliana AAM61251 and UniRef100_Q8LFR4 90 polynucleotide AF506366.1 | codes Nicotiana tabacum AAM48129 and UniRef100_Q8LKR4 91 polynucleotide AF428294.1 | codes Arabidopsis thaliana AAL16126 and UniRef100_Q944L6 92 polynucleotide AF353615.1 | codes Nicotiana tabacum AAK38667 and UniRef100_Q94KK8 93 polynucleotide AB056061.1 | codes Oryza sativa Japonica BAB32869 and UniRef100_Q9AR41 94 polynucleotide AB056061.1 | codes Oryza sativa Japonica BAB32869 and UniRef100_Q9AR41 95 polynucleotide AF352732.1 | codes Nicotiana tabacum AAK18620 and UniRef100_Q9AT17 96 polynucleotide AB026646.1 | codes Arabidopsis thaliana BAB02870 and UniRef100_Q9LSH2 97 polypeptide XP_462650 Oryza sativa 98 polypeptide AAN46801.1 Arabidopsis thaliana 99 polypeptide XP_482841.1 Oryza sativa 100 polypeptide AAS79671.1 Brassica juncea 101 polypeptide AAS79670.1 Brassica juncea 102 polypeptide AAS79669.1 Brassica juncea 103 polypeptide BAB02870.1 Arabidopsis thaliana 104 polypeptide AAM70569.1 Arabidopsis thaliana 105 polypeptide BAC42751.1 Arabidopsis thaliana 106 polypeptide AAK38667.1 | AF353615_1 Nicotiana tabacum 107 polypeptide AAK18620.1 | AF352732_1 Nicotiana tabacum 108 polypeptide AAP85548.1 Glycine max 109 polypeptide AAM48129.1 | AF506366_1 Nicotiana tabacum 110 polypeptide AAM61251.1 Arabidopsis thaliana 111 polypeptide AAP79441.1 Oryza sativa 112 polypeptide AAL91148.1 Arabidopsis thaliana 113 polypeptide XP_482840.1 Oryza sativa 114 polypeptide AAL16126.1 | AF428294_1 Arabidopsis thaliana 115 polypeptide AAC39483.1 Nicotiana tabacum 116 polypeptide AAM70582.1 Arabidopsis thaliana 117 polypeptide AAC24195.1 Nicotiana tabacum 118 polypeptide AAB40608.1 Nicotiana tabacum 119 polypeptide AAA93132.1 Arabidopsis thaliana 120 polypeptide A48767 Petunia × hybrida 121 polypeptide AAL83983.1 Oryza sativa 122 polypeptide CAA56812.1 Lycopersicon esculentum 123 polypeptide XP_46254.1 Oryza sativa 124 polypeptide AAM47304.1 | AF377946_6 Oryza sativa 125 polypeptide BAB32871.1 Oryza sativa 126 polypeptide AAT77842.1 Oryza sativa 127 polypeptide AAP46640.1 Hordeum vulgare

Sequence CWU 1

1271502PRTLolium perenne 1Met Val Leu Thr Val Ala Ala Thr Ala Ala Asp Thr Ala Glu Pro Leu1 5 10 15Asn Ser Thr Phe Phe Ala Thr Arg Tyr Val Arg Asp Gln Leu Pro Arg 20 25 30Tyr Arg Met Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile 35 40 45Ile Ser Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala 50 55 60Ser Phe Val Thr Thr Arg Met Glu Pro Glu Val Gly Lys Leu Ile Met65 70 75 80Asp Ser Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr 85 90 95Thr Glu Leu Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn 100 105 110Ala Pro Ile Lys Glu Glu Glu Thr Ala Ile Gly Val Ala Thr Val Gly 115 120 125Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp 130 135 140Ala Asn Lys Arg Lys Glu Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile145 150 155 160Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr 165 170 175Phe Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Tyr Tyr Val 180 185 190Met Asp Pro Leu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys 195 200 205Val Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Tyr Glu Asp Val 210 215 220Lys Leu Leu Asn Asp Leu Leu Val Glu Lys Asn Lys Lys Thr Gly Phe225 230 235 240Asn Val Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro 245 250 255Phe Leu His Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys 260 265 270Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly Val 275 280 285Gly Trp Val Ile Trp Arg Ser Lys Asp Asp Leu Pro Gly Glu Leu Ile 290 295 300Phe His Ile Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu Asn305 310 315 320Phe Ser Lys Gly Ala Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile 325 330 335Arg Leu Gly Phe Glu Gly Tyr Lys His Ile Met Glu Asn Cys Gln Ala 340 345 350Asn Ala Thr Ala Leu Arg Glu Gly Leu Glu Ala Thr Gly Arg Phe Asp 355 360 365Ile Leu Ser Lys Glu Asp Gly Val Pro Leu Val Ala Ile Arg Leu Lys 370 375 380Asp Ser Ser Lys Phe Ser Val Phe Asp Ile Ser Glu Asn Leu Arg Arg385 390 395 400Phe Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Glu His 405 410 415Val Ala Val Leu Arg Val Val Ile Arg Glu Asp Phe Asn Arg Ser Leu 420 425 430Ser Gln Arg Leu Leu Ala Asp Ile Asn Arg Val Val Gln Glu Leu Asp 435 440 445Ala His Ala Val His Ala Ile Lys Met Thr Thr Ala Ile Ala Thr Gln 450 455 460Thr Gly Glu Gly Ala Glu Asp Gly Val Val Thr Lys Lys Gly Val Leu465 470 475 480Asp Ile Glu Lys Glu Phe Ala Ala Ala Cys Lys Asp Leu Val Lys Asn 485 490 495Lys Lys Thr Gly Pro Cys 5002502PRTArabidopsis thaliana 2Met Val Leu Ser His Ala Val Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ser Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Ala Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Val Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Gln Gln Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335His Glu Gly Tyr Arg Asn Val Met Glu Asn Cys Arg Glu Asn Met Ile 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Glu Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Cys His Thr Glu Phe Glu Ile Ser Asp Met Leu Arg Arg Tyr Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Met Arg Glu Leu Asp Glu Leu Pro 435 440 445Ser Arg Val Ile His Lys Ile Ser Leu Gly Gln Glu Lys Ser Glu Ser 450 455 460Asn Ser Asp Asn Leu Met Val Thr Val Lys Lys Ser Asp Ile Asp Lys465 470 475 480Gln Arg Asp Ile Ile Thr Gly Trp Lys Lys Phe Val Ala Asp Arg Lys 485 490 495Lys Thr Ser Gly Ile Cys 5003494PRTArabidopsis thaliana 3Met Val Leu Thr Lys Thr Ala Thr Asn Asp Glu Ser Val Cys Thr Met1 5 10 15Phe Gly Ser Arg Tyr Val Arg Thr Thr Leu Pro Lys Tyr Glu Ile Gly 20 25 30Glu Asn Ser Ile Pro Lys Asp Ala Ala Tyr Gln Ile Ile Lys Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Ile Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala Pro Leu Glu 100 105 110Glu Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys Arg 130 135 140Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Asn Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro Asp 180 185 190Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu Asn 210 215 220Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly Trp Asn Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr Pro 245 250 255Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val Val 275 280 285Trp Arg Ala Ala Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Ile Glu Asn Met Val Val 340 345 350Leu Lys Glu Gly Ile Glu Lys Thr Glu Arg Phe Asn Ile Val Ser Lys 355 360 365Asp Gln Gly Val Pro Val Val Ala Phe Ser Leu Lys Asp His Ser Phe 370 375 380His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg Arg Phe Gly Trp Ile385 390 395 400Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Thr Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg Leu 420 425 430Val Ala Asp Ile Ser Lys Val Leu His Glu Leu Asp Thr Leu Pro Ser 435 440 445Lys Ile Ser Lys Lys Met Gly Ile Glu Gly Ile Ala Glu Asn Val Lys 450 455 460Glu Lys Lys Met Glu Lys Glu Ile Leu Met Glu Val Ile Val Gly Trp465 470 475 480Arg Lys Phe Val Lys Glu Arg Lys Lys Met Asn Gly Val Cys 485 4904500PRTPetunia hybrida 4Met Val Leu Ser Lys Thr Val Ser Gln Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Asp Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Glu Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Asn Lys Asp Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Tyr Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Ser 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Arg 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Arg Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Glu Gln Ala Ala Ala 450 455 460Asn Gly Ser Glu Val His Lys Lys Thr Asp Ser Glu Val Gln Leu Glu465 470 475 480Met Ile Thr Ala Trp Lys Lys Phe Val Glu Glu Lys Lys Lys Lys Thr 485 490 495Asn Arg Val Cys 5005502PRTLycopersicon esculentum 5Met Val Leu Thr Thr Thr Ser Ile Arg Asp Ser Glu Glu Ser Leu His1 5 10 15Cys Thr Phe Ala Ser Arg Tyr Val Gln Glu Pro Leu Pro Lys Phe Lys 20 25 30Met Pro Lys Lys Ser Met Pro Lys Glu Ala Ala Tyr Gln Ile Val Asn 35 40 45Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe 50 55 60Val Ser Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ser Ser65 70 75 80Ile Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu 85 90 95Leu Gln Asn Arg Cys Val Asn Met Leu Ala His Leu Phe His Ala Pro 100 105 110Val Gly Asp Asp Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser 115 120 125Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Ser 130 135 140Lys Arg Lys Ala Glu Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr145 150 155 160Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu 165 170 175Val Glu Leu Lys Glu Val Lys Leu Lys Glu Gly Tyr Tyr Val Met Asp 180 185 190Pro Ala Lys Ala Val Glu Ile Val Asp Glu Asn Thr Ile Cys Val Ala 195 200 205Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu 210 215 220Leu Asn Glu Leu Leu Thr Lys Lys Asn Lys Glu Thr Gly Trp Glu Thr225 230 235 240Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu 245 250 255Trp Pro Asp Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile 260 265 270Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp 275 280 285Val Ile Trp Arg Ser Lys Glu Asp Leu Pro Asp Glu Leu Val Phe His 290 295 300Ile Asn Tyr Leu Gly Ser Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser305 310 315 320Lys Gly Ser Tyr Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu 325 330 335Gly Phe Glu Gly Tyr Lys Asn Val Met Lys Asn Cys Leu Ser Asn Ala 340 345 350Lys Val Leu Thr Glu Gly Ile Thr Lys Met Gly Arg Phe Asp Ile Val 355 360 365Ser Lys Asp Val Gly Val Pro Val Val Ala Phe Ser Leu Arg Asp Ser 370 375 380Ser Lys Tyr Thr Val Phe Glu Val Ser Glu His Leu Arg Arg Phe Gly385 390 395 400Trp Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Glu His Ile Ala 405 410 415Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser His Ser Leu Ala Glu 420 425 430Arg Leu Val Ser Asp Ile Glu Lys Ile Leu Ser Glu Leu Asp Thr Gln 435 440 445Pro Pro Arg Leu Pro Thr Lys Ala Val Arg Val Thr Ala Glu Glu Val 450

455 460Arg Asp Asp Lys Gly Asp Gly Leu His His Phe His Met Asp Thr Val465 470 475 480Glu Thr Gln Lys Asp Ile Ile Lys His Trp Arg Lys Ile Ala Gly Lys 485 490 495Lys Thr Ser Gly Val Cys 5006499PRTPopulus tremula x Populus alba 6Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Ala Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Phe Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Ile Ala Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Gln Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Ser Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Tyr Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Arg Asp Asn Met Leu 340 345 350Val Leu Lys Gln Gly Leu Glu Lys Thr Gly Lys Phe Asn Ile Val Ser 355 360 365Lys Asp Lys Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Leu His Asn Glu Phe Glu Val Ser Asp Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Gly Lys Val Leu His Glu Leu Glu Thr Leu Pro 435 440 445Ser Arg Ile Ser Ala Lys Ile Val Leu Ala Asn Glu Glu Lys Asp Ala 450 455 460Val Ala Ala Gly Lys Glu Lys Lys Asp Val Gln Asn Glu Thr Arg Glu465 470 475 480Ile Ile Thr Ala Trp Arg Lys Leu Val Val Gln Arg Lys Lys Leu Asn 485 490 495Gly Val Cys7492PRTOryza sativa subsp. indica 7Met Val Leu Ser Lys Ala Val Ser Glu Ser Asp Met Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Ala Ser Leu Pro Arg Tyr Arg Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ala Ala Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe His Ala Pro Leu 100 105 110Gly Glu Asp Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Phe Asp Lys Pro Asn Ile Ile Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Asp Lys Lys Asn Lys Glu Thr Gly Trp Glu Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Cys 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg His Gly 325 330 335Phe Glu Gly Tyr Arg Asn Ile Met Glu Asn Cys His Glu Asn Ala Met 340 345 350Val Leu Lys Glu Gly Leu Val Lys Thr Gly Arg Phe Asp Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Arg Ser 370 375 380Arg His Asp Glu Phe Glu Ile Ser Asp Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Glu Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Ile Glu Lys Val Met Tyr Gln Leu Asp Ala Leu Pro 435 440 445Ser Arg Leu Met Pro Pro Val Pro Pro Ala Pro Leu Leu Val Val Ala 450 455 460Lys Lys Ser Glu Leu Glu Thr Gln Arg Ser Val Thr Glu Ala Trp Lys465 470 475 480Lys Phe Val Leu Ala Lys Arg Thr Asn Gly Val Cys 485 4908461PRTOryza sativa subsp. japonica 8Met Pro Glu Gln Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn1 5 10 15Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe 20 25 30Val Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Gln Ala Ser 35 40 45Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu 50 55 60Leu Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro65 70 75 80Leu Gly Asp Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser 85 90 95Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln Asn 100 105 110Lys Met Lys Ala Ala Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr 115 120 125Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu 130 135 140Val Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp145 150 155 160Pro Ala Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala 165 170 175Ala Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu 180 185 190Leu Asn Asp Leu Leu Thr Lys Lys Asn Ala Glu Thr Gly Trp Asp Thr 195 200 205Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu 210 215 220Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile225 230 235 240Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp 245 250 255Cys Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His 260 265 270Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser 275 280 285Lys Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu 290 295 300Gly Phe Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Gln Glu Asn Ala305 310 315 320Met Val Leu Lys Gln Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val 325 330 335Ser Lys Asp Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser 340 345 350Ala Arg His Asn Glu Phe Glu Ile Ser Asp Phe Leu Arg Arg Phe Gly 355 360 365Trp Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr 370 375 380Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu385 390 395 400Arg Leu Val Leu Asp Val Glu Lys Val Leu His Glu Leu Asp Ala Leu 405 410 415Pro Ala Arg Val Val Ala Asn Gly Asp Asn Pro Ala Ala Ala Ser Ala 420 425 430Ser Glu Arg Glu Met Glu Lys Gln Arg Glu Val Ile Ser Leu Trp Lys 435 440 445Arg Ala Val Leu Ala Lys Lys Lys Thr Asn Gly Val Cys 450 455 4609489PRTVitis viniferamisc_feature(229)..(229)Xaa can be any naturally occurring amino acid 9Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Lys Ala Ser Leu Pro Arg Phe Lys Leu 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Ala Ala Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Asn Arg Cys Val Asn 85 90 95Ile Ile Ala His Leu Phe Asn Ala Pro Leu Glu Asp Ser Glu Ala Ala 100 105 110Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala Ile Met Leu Ala Gly 115 120 125Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys Arg Lys Ala Glu Gly Lys 130 135 140Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly Ala Asn Val Gln Val Cys145 150 155 160Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu Leu Lys Glu Val Lys 165 170 175Leu Arg Asp Gly Tyr Tyr Val Met Asp Pro Glu Lys Ala Val Glu Met 180 185 190Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile Leu Gly Ser Thr Leu 195 200 205Asn Gly Glu Phe Glu Asp Val Lys Leu Leu Asn Asp Leu Leu Val Glu 210 215 220Lys Asn Lys Gln Xaa Gly Trp Asp Thr Pro Ile His Val Asp Ala Ala225 230 235 240Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr Pro Glu Leu Glu Trp Asp 245 250 255Phe Arg Leu Pro Leu Val Lys Ser Ile Asn Val Ser Gly His Lys Tyr 260 265 270Gly Leu Val Tyr Ala Gly Ile Gly Trp Val Val Trp Arg Ser Lys Glu 275 280 285Asp Leu Pro Glu Glu Leu Ile Phe His Ile Asn Tyr Leu Gly Ala Asp 290 295 300Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly Ser Ser Gln Val Ile305 310 315 320Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe Glu Gly Tyr Arg Asn 325 330 335Val Met Glu Asn Cys Gln Glu Asn Ala Met Ala Leu Lys Glu Gly Leu 340 345 350Glu Lys Thr Gly Arg Phe Asn Ile Ile Ser Lys Asp Asn Gly Val Pro 355 360 365Leu Val Ala Phe Ser Leu Lys Asp Asn Ser Cys His Asp Glu Phe Glu 370 375 380Val Ala Asp Met Leu Arg Arg Phe Gly Trp Ile Val Pro Ala Tyr Thr385 390 395 400Met Pro Pro Asp Ala Gln His Val Thr Val Leu Arg Val Val Val Arg 405 410 415Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg Leu Val Phe Asp Ile Thr 420 425 430Lys Val Leu His Glu Leu Asp Met Leu Pro Ala Lys Leu Ser Ala Lys 435 440 445Ile Ser Val Glu Glu Lys Lys Gln Asn Gly Thr Ile Leu Lys Lys Ser 450 455 460Val Ile Glu Thr Gln Arg Glu Ile Thr Asp Ala Trp Lys Lys Phe Val465 470 475 480Met Ala Lys Lys Thr Asn Gly Val Cys 48510495PRTVitis vinifera 10Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Lys Ala Ser Leu Pro Arg Phe Lys Leu 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Ala Ala Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Ile Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Glu Asp Ser Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Glu Asn Cys Gln Glu Asn Ala Met 340 345 350Ala Leu Lys Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Asp Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Cys His Asp Glu Phe Glu Val Ala Asp Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Val Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Phe Asp Ile Thr Lys Val Leu His Glu Leu Asp Met Leu Pro 435 440 445Ala Lys Leu Ser Ala Lys Ile Ser Val Glu Glu Lys Lys Gln Asn Gly 450 455 460Thr Ile Leu Lys Lys Ser Val Ile

Glu Thr Gln Arg Glu Ile Thr Asp465 470 475 480Ala Trp Lys Lys Phe Val Met Ala Lys Lys Thr Asn Gly Val Cys 485 490 49511476PRTVitis vinifera 11Met Pro Glu Lys Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Val His1 5 10 15Asp Glu Leu Leu Leu Asp Gly Leu Pro Arg Leu Asn Leu Ala Thr Phe 20 25 30Val Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Ala Glu Ala 35 40 45Ile Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu 50 55 60Leu Gln Asn Arg Cys Val Asn Met Ile Ala Lys Leu Phe Asn Ala Pro65 70 75 80Ser Ala Asp Gln Thr Lys Gln Ala Val Gly Val Gly Thr Val Gly Ser 85 90 95Ser Glu Ala Met Met Leu Ala Gly Leu Ala Phe Lys Lys Lys Trp Gln 100 105 110Asn Lys Arg Lys Ala Gln Lys Lys Pro Phe Asp Lys Pro Asn Ile Val 115 120 125Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe 130 135 140Glu Val Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met145 150 155 160Asp Pro Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val 165 170 175Ala Ala Ile Leu Gly Ser Thr Phe Asn Gly Glu Phe Glu Asp Val Lys 180 185 190Leu Leu Asn Thr Leu Leu Thr Gln Lys Asn Lys Arg Thr Gly Trp Asp 195 200 205Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Val Ala Pro Phe 210 215 220Leu Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser225 230 235 240Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly 245 250 255Trp Ala Ile Trp Arg Ser Lys Glu Glu Leu Pro Glu Glu Leu Ile Phe 260 265 270His Ile Asn Tyr Leu Gly Gly Asp Glu Pro Thr Phe Thr Leu Asn Phe 275 280 285Ser Lys Gly Asn Gln Val Ile Ala Gln Tyr Tyr Gln Phe Leu Arg Met 290 295 300Gly Phe Glu Gly Tyr Lys Lys Val Met Ser Asn Cys Met Glu Ser Ala305 310 315 320Arg Ile Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Gln Ile Ile 325 330 335Ser Lys Glu Lys Gly Val Pro Val Val Ala Phe Ala Phe Lys Gly Asn 340 345 350Asp Arg Lys Asn Leu Ala Phe Gly Leu Ser Lys Ala Leu Arg Asn Tyr 355 360 365Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asn Ala Glu Asn Val 370 375 380Thr Val Leu Arg Val Val Val Arg Glu Asp Phe Gly Arg Gln Leu Val385 390 395 400Glu Lys Leu Leu Phe His Ile Gly Val Ala Leu Lys Glu Val Thr Asp 405 410 415Ala Ala Ser Ser Val Pro Met Ile Arg Leu Thr Val Glu Met Lys Ala 420 425 430Asp Glu Ser Glu Met Asn Ala Gly Glu Gly Thr Leu His Ile Pro Ala 435 440 445Ala Ser Val His Trp Lys His Asp Lys Pro Glu Thr Val Asp Thr Gln 450 455 460Val Pro Ile Met Asp Gly Lys Thr Lys Gly Val Cys465 470 47512496PRTNicotiana tabacum 12Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Thr Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Glu Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Ile Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Leu Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Phe His Gly Val Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Leu Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 49513496PRTNicotiana tabacum 13Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Ile Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 49514496PRTNicotiana tabacum 14Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Glu Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Ile Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 49515500PRTOryza sativa 15Met Val Leu Thr His Val Glu Ala Val Glu Glu Gly Ser Glu Ala Ala1 5 10 15Ala Ala Val Phe Ala Ser Arg Tyr Val Gln Asp Pro Val Pro Arg Tyr 20 25 30Glu Leu Gly Glu Arg Ser Ile Ser Lys Asp Ala Ala Tyr Gln Ile Val 35 40 45His Asp Glu Leu Leu Leu Asp Ser Ser Pro Arg Leu Asn Leu Ala Ser 50 55 60Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Arg Leu Ile Leu Glu65 70 75 80Ala Ile Asn Lys Asn Tyr Ala Asp Met Asp Glu Tyr Pro Val Thr Thr 85 90 95Glu Leu Gln Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala 100 105 110Pro Val Gly Asp Gly Glu Lys Ala Val Gly Val Gly Thr Val Gly Ser 115 120 125Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln 130 135 140Asn Arg Arg Lys Ala Ala Gly Lys Pro His Asp Lys Pro Asn Ile Val145 150 155 160Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe 165 170 175Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Cys Tyr Val Met 180 185 190Asp Pro Val Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val 195 200 205Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Arg 210 215 220Arg Leu Asn Asp Leu Leu Ala Ala Lys Asn Lys Arg Thr Gly Trp Asp225 230 235 240Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe 245 250 255Ile Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser 260 265 270Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly 275 280 285Trp Val Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe 290 295 300His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe305 310 315 320Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Leu Arg 325 330 335Leu Gly Phe Glu Gly Tyr Lys Ser Val Met Lys Asn Cys Met Glu Ser 340 345 350Ala Arg Thr Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Thr Ile 355 360 365Ile Ser Lys Glu Glu Gly Val Pro Leu Val Ala Phe Thr Phe Lys Asp 370 375 380Gly Ala Gly Ala Gln Ala Phe Arg Leu Ser Ser Gly Leu Arg Arg Tyr385 390 395 400Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Ala Leu Glu His Met 405 410 415Thr Val Leu Arg Val Val Val Arg Glu Asp Phe Gly Arg Pro Leu Ala 420 425 430Glu Arg Phe Leu Ser His Val Arg Met Ala Leu Asp Glu Met Asp Leu 435 440 445Ala Ala Arg Ala Pro Val Pro Arg Val Gln Leu Thr Ile Glu Leu Gly 450 455

460Pro Ala Arg Thr Ala Gly Glu Glu Ala Ser Ile Arg Val Val Lys Ser465 470 475 480Glu Ala Val Pro Val Arg Lys Ser Val Pro Leu Val Ala Gly Lys Thr 485 490 495Lys Gly Val Cys 50016494PRTCitrus sinensis 16Met Val Leu Ser Lys Thr Phe Ser Glu Ser Asp Glu Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Asn Ser Leu Pro Arg Phe Thr Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Ala Ala Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Glu Asp Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Ala Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Thr Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Glu Asn Cys His Glu Asn Ala Met 340 345 350Val Leu Lys Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Lys 370 375 380Arg His Asp Glu Phe Glu Val Ala Glu Leu Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Ile Thr Lys Val Leu His Glu Leu Asp Ser Leu Pro 435 440 445Ser Lys Val Leu Val Pro Ala Ser Glu Gln Asn Gly Arg Asn Gly Lys 450 455 460Lys Thr Glu Ile Glu Thr Gln Arg Glu Val Thr Thr Tyr Trp Arg Lys465 470 475 480Phe Val Ser Glu Arg Lys Ala Asn Asn Lys Asn Lys Ile Cys 485 49017492PRTOryza sativa subsp. japonica 17Met Val Leu Ser His Gly Val Ser Gly Ser Asp Glu Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Arg Met 20 25 30Pro Glu Gln Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Gln Ala Ser Val65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln Asn Lys 130 135 140Met Lys Ala Ala Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Ala Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Thr Lys Lys Asn Ala Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Cys 275 280 285Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Gln Glu Asn Ala Met 340 345 350Val Leu Lys Gln Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ala 370 375 380Arg His Asn Glu Phe Glu Ile Ser Asp Phe Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Val Glu Lys Val Leu His Glu Leu Asp Ala Leu Pro 435 440 445Ala Arg Val Val Ala Asn Gly Gly Asp Ala Ala Ala Ala Ser Ala Ser 450 455 460Glu Arg Glu Met Glu Lys Gln Arg Glu Val Ile Ser Leu Trp Lys Arg465 470 475 480Ala Val Leu Ala Lys Lys Lys Thr Asn Gly Val Cys 485 49018493PRTBrassica juncea 18Met Val Leu Ser Lys Thr Ala Ser Gly Thr Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Asn Ser Leu Pro Arg Phe Glu Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Gln Gly Leu Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Gly225 230 235 240Asn His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Ser Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asn Glu Phe Glu Val Ala Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Val Pro Ala Asp Ala Glu His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Arg Ala Lys Met Ala Asn Gly Lys Ala Lys Val Val Lys 450 455 460Gln Thr Glu Glu Glu Thr Thr Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Phe Val Glu Thr Lys Lys Thr Asn Gln Asn Lys Ile Cys 485 49019493PRTBrassica juncea 19Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Glu Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Gln Gly Leu Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Gly225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Ser Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asp Glu Phe Glu Val Ala Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Gln Ala Lys Met Ala Asn Gly Asn Ala Asn Gly Val Lys 450 455 460Lys Thr Glu Glu Glu Thr Thr Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Phe Val Glu Ala Lys Lys Ser Asn Lys Asn Arg Ile Cys 485 49020494PRTBrassica juncea 20Met Val Leu Ser Arg Ala Ala Thr Glu Ser Gly Glu Asn Val Cys Ser1 5 10 15Thr Phe Gly Ser Arg Tyr Val Arg Thr Ala Leu Pro Lys His Lys Ile 20 25 30Gly Glu Ser Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Lys Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Thr Glu Thr Ala Met Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Asn Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Asp Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly Trp Asn Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Gln Gln Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Arg Glu Asn Met Val 340 345 350Val Leu Arg Glu Gly Ile Glu Lys Thr Glu Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Val Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp His Ser 370 375 380Phe His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Ile Val Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440

445Ser Lys Ile Ser Lys Lys Met Gly Ala Glu Asp Phe Gly Asn Val Lys 450 455 460Gly Lys Lys Val Asp Arg Asp Val Leu Met Glu Val Ile Val Gly Trp465 470 475 480Arg Lys Phe Val Lys Asp Arg Lys Lys Met Asn Gly Val Cys 485 49021497PRTOryza sativa subsp. japonica 21Met Val Leu Ser His Ala Ser Ser Gly Arg Asp Asp Ala Val Arg Cys1 5 10 15Thr Phe Ala Thr Arg Tyr Ala Cys Glu Thr Leu Pro Arg Phe Arg Met 20 25 30Pro Glu Gln Ser Ile Pro Arg Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Val65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Ile 100 105 110Lys Glu Asp Glu Thr Ala Ile Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Glu Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asn Leu Leu Thr Glu Lys Asn Lys Glu Thr Gly Trp Asp Val Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly Val Gly Trp Val 275 280 285Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Ile Met Gln Asn Cys Met Glu Asn Thr Ala 340 345 350Ile Leu Arg Glu Gly Ile Glu Ala Thr Gly Arg Phe Glu Ile Leu Ser 355 360 365Lys Glu Ala Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Gly 370 375 380Arg Tyr Thr Val Phe Asp Ile Ser Glu His Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asn Ala Glu His Val Ala Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Ser Leu Ala Glu Arg 420 425 430Leu Val Ser Asp Ile Val Lys Ile Leu His Glu Leu Asp Ala His Ser 435 440 445Ala Gln Val Leu Lys Ile Ser Ser Ala Ile Ala Lys Gln Gln Ser Gly 450 455 460Asp Asp Gly Val Val Thr Lys Lys Ser Val Leu Glu Thr Glu Arg Glu465 470 475 480Ile Phe Ala Tyr Trp Arg Asp Gln Val Lys Lys Lys Gln Thr Gly Ile 485 490 495Cys22490PRTHordeum vulgare 22Met Val Val Thr Val Ala Ala Thr Gly Pro Asp Thr Ala Glu Thr Leu1 5 10 15His Ser Thr Thr Phe Ala Ser Arg Tyr Val Arg Asp Gln Leu Pro Arg 20 25 30Tyr Arg Met Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile 35 40 45Ile Ser Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala 50 55 60Ser Phe Val Thr Thr Trp Met Glu Pro Glu Cys Gly Lys Leu Ile Met65 70 75 80Asp Ser Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr 85 90 95Thr Glu Leu Gln Asp Arg Cys Val Asn Met Ile Ala His Leu Phe Asn 100 105 110Ala Pro Ile Gly Glu Asp Glu Thr Ala Ile Gly Val Ser Thr Val Gly 115 120 125Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp 130 135 140Ala Asn Lys Met Lys Glu Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile145 150 155 160Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr 165 170 175Phe Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Tyr Tyr Val 180 185 190Met Asp Pro Lys Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys 195 200 205Val Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Tyr Glu Asp Val 210 215 220Lys Leu Leu Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp225 230 235 240Asn Val Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro 245 250 255Phe Leu Gln Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys 260 265 270Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly Val 275 280 285Gly Trp Val Ile Trp Arg Ser Lys Asp Asp Leu Pro Glu Glu Leu Ile 290 295 300Phe His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn305 310 315 320Phe Ser Lys Gly Gln Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg 325 330 335Leu Gly Phe Glu Gly Tyr Lys His Ile Met Glu Asn Cys Lys Leu Asn 340 345 350Ala Ala Val Leu Lys Glu Gly Ile Asp Ala Thr Gly Arg Phe Asp Val 355 360 365Leu Ser Lys Ala Asp Gly Val Pro Leu Val Ala Ile Arg Leu Lys Asp 370 375 380Ser Thr Asn Phe Ser Val Phe Asp Ile Ser Glu Asn Leu Arg Arg Phe385 390 395 400Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Glu His Val 405 410 415Ala Val Leu Arg Ile Val Ile Arg Glu Asp Phe Asn Arg Ser Leu Ala 420 425 430Gln Arg Leu Leu Ala Asp Ile Asn Lys Ile Ile Gly Glu Leu Asp Ala 435 440 445His Ala Val His Ala Ile Lys Leu Ser Thr Ala Ala Ala Gly Gly Asp 450 455 460Gly Ala Ser Lys Ser Ala Val Asp Ala Ala Thr Glu Ala Phe Lys Asp465 470 475 480Leu Ala Gly Lys Lys Lys Ala Gly Val Cys 485 49023492PRTOryza sativa subsp. japonica 23Met Val Leu Ser Lys Ala Val Ser Glu Ser Asp Met Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Ala Ser Leu Pro Arg Tyr Arg Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ala Ala Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe His Ala Pro Leu 100 105 110Gly Glu Asp Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Phe Asp Lys Pro Asn Ile Ile Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Asp Met Val Asn Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Asp Lys Lys Asn Lys Glu Thr Gly Trp Glu Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Cys 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg His Gly 325 330 335Phe Glu Gly Tyr Arg Asn Ile Met Glu Asn Cys His Glu Asn Ala Met 340 345 350Val Leu Lys Glu Gly Leu Val Lys Thr Gly Arg Phe Asp Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Arg Ser 370 375 380Arg His Asp Glu Phe Glu Ile Ser Asp Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Glu Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Ile Glu Lys Val Met Tyr Gln Leu Asp Ala Leu Pro 435 440 445Ser Arg Leu Met Pro Pro Val Pro Pro Ala Pro Leu Leu Val Val Ala 450 455 460Lys Lys Ser Glu Leu Glu Thr Gln Arg Ser Val Thr Glu Ala Trp Lys465 470 475 480Lys Phe Val Leu Ala Lys Arg Thr Asn Gly Val Cys485 49024494PRTArabidopsis thaliana 24Met Val Leu Ala Thr Asn Ser Asp Ser Asp Glu His Leu His Ser Thr1 5 10 15Phe Ala Ser Arg Tyr Val Arg Ala Val Val Pro Arg Phe Lys Met Pro 20 25 30Asp His Cys Met Pro Lys Asp Ala Ala Tyr Gln Val Ile Asn Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Val Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Met Ile Ala Asn Phe Phe His Ala Pro Val Gly 100 105 110Glu Asp Glu Ala Ala Ile Gly Cys Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln His Arg Arg 130 135 140Lys Ala Gln Gly Leu Pro Ile Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Lys Leu Ser Glu Asp Tyr Tyr Val Met Asp Pro Ala 180 185 190Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Gln Leu Asn 210 215 220Asp Leu Leu Ala Glu Lys Asn Ala Glu Thr Gly Trp Glu Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr Pro 245 250 255Asp Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp Val Val 275 280 285Trp Arg Thr Lys Asp Asp Leu Pro Glu Glu Leu Val Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Met Asp Asn Ala Arg Arg 340 345 350Leu Arg Glu Gly Ile Glu Met Thr Gly Lys Phe Asn Ile Val Ser Lys 355 360 365Asp Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser Lys 370 375 380His Thr Val Phe Glu Ile Ala Glu Ser Leu Arg Lys Phe Gly Trp Ile385 390 395 400Ile Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Ala Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Gly Leu Ala Asp Arg Leu 420 425 430Ile Thr His Ile Ile Gln Val Leu Lys Glu Ile Glu Gly Leu Pro Ser 435 440 445Arg Ile Ala His Leu Ala Ala Ala Ala Ala Val Ser Gly Asp Asp Glu 450 455 460Glu Val Lys Val Lys Thr Ala Lys Met Ser Leu Glu Asp Ile Thr Lys465 470 475 480Tyr Trp Lys Arg Leu Val Glu His Lys Arg Asn Ile Val Cys 485 49025496PRTNicotiana tabacum 25Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Thr Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Ile Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His

Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 49526494PRTArabidopsis thaliana 26Met Val Leu Thr Lys Thr Ala Thr Asn Asp Glu Ser Val Cys Thr Met1 5 10 15Phe Gly Ser Arg Tyr Val Arg Thr Thr Leu Pro Lys Tyr Glu Ile Gly 20 25 30Glu Asn Ser Ile Pro Lys Asp Ala Ala Tyr Gln Ile Ile Lys Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Ile Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala Pro Leu Glu 100 105 110Glu Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys Arg 130 135 140Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Asn Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro Asp 180 185 190Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu Asn 210 215 220Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly Trp Asn Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr Pro 245 250 255Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val Val 275 280 285Trp Arg Ala Ala Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Ile Glu Asn Met Val Val 340 345 350Leu Lys Glu Gly Ile Glu Lys Thr Glu Arg Phe Asn Ile Val Ser Lys 355 360 365Asp Gln Gly Val Pro Val Val Ala Phe Ser Leu Lys Asp His Ser Phe 370 375 380His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg Arg Phe Gly Trp Ile385 390 395 400Val Pro Ala Tyr Thr Met Pro Ala Asp Val Gln His Ile Thr Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg Leu 420 425 430Val Ala Asp Ile Ser Lys Val Leu His Glu Leu Asp Thr Leu Pro Ser 435 440 445Lys Ile Ser Lys Lys Met Gly Ile Glu Gly Ile Ala Glu Asn Val Lys 450 455 460Glu Lys Lys Met Glu Lys Glu Ile Leu Met Glu Val Ile Val Gly Trp465 470 475 480Arg Lys Phe Val Lys Glu Arg Lys Lys Met Asn Gly Val Cys 485 49027491PRTNicotiana tabacum 27Met Val Leu Ser Lys Thr Ser Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Glu Met 20 25 30Ala Glu Asn Ser Ile Pro Lys Glu Ala Ala Phe Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Lys Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Asn Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Tyr Asn Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Asn Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Val Gln Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Gln Thr Gly Trp Asn Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Ile Trp Arg Thr Lys Gln Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Tyr Glu Gly Tyr Arg Asn Val Met Glu Asn Cys Arg Glu Asn Ala Ile 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Arg His Asn Glu Phe Glu Val Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Ile Val Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Leu Ser Ala Lys Leu Glu Glu Val Lys Leu Val Lys Asn Gly 450 455 460Lys Lys Phe Glu Leu Glu Val Gln Arg Glu Val Thr Asn Tyr Trp Lys465 470 475 480Lys Phe Val Leu Ala Arg Lys Ala Pro Val Cys 485 49028501PRTOryza sativa subsp. japonica 28Met Val Val Ser Val Ala Ala Thr Asp Ser Asp Thr Ala Gln Pro Val1 5 10 15Gln Tyr Ser Thr Phe Phe Ala Ser Arg Tyr Val Arg Asp Pro Leu Pro 20 25 30Arg Phe Arg Met Pro Glu Gln Ser Ile Pro Arg Glu Ala Ala Tyr Gln 35 40 45Ile Ile Asn Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu 50 55 60Ala Ser Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile65 70 75 80Met Asp Ser Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val 85 90 95Thr Thr Glu Leu Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe 100 105 110Asn Ala Pro Ile Lys Glu Asp Glu Thr Ala Ile Gly Val Gly Thr Val 115 120 125Gly Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys 130 135 140Trp Gln Asn Lys Arg Lys Glu Gln Gly Lys Pro Cys Asp Lys Pro Asn145 150 155 160Ile Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg 165 170 175Tyr Phe Glu Val Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr 180 185 190Val Met Asp Pro Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile 195 200 205Cys Val Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp 210 215 220Val Lys Leu Leu Asn Asn Leu Leu Thr Glu Lys Asn Lys Glu Thr Gly225 230 235 240Trp Asp Val Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala 245 250 255Pro Phe Leu Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val 260 265 270Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly 275 280 285Val Gly Trp Val Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu 290 295 300Ile Phe His Ile Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu305 310 315 320Asn Phe Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu 325 330 335Ile Arg Leu Gly Phe Glu Gly Tyr Lys Asn Ile Met Gln Asn Cys Met 340 345 350Glu Asn Thr Ala Ile Leu Arg Glu Gly Ile Glu Ala Thr Gly Arg Phe 355 360 365Glu Ile Leu Ser Lys Glu Ala Gly Val Pro Leu Val Ala Phe Ser Leu 370 375 380Lys Asp Ser Gly Arg Tyr Thr Val Phe Asp Ile Ser Glu His Leu Arg385 390 395 400Arg Phe Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asn Ala Glu 405 410 415His Val Ala Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Ser 420 425 430Leu Ala Glu Arg Leu Val Ser Asp Ile Val Lys Ile Leu His Glu Leu 435 440 445Asp Ala His Ser Ala Gln Val Leu Lys Ile Ser Ser Ala Ile Ala Lys 450 455 460Gln Gln Ser Gly Asp Asp Gly Val Val Thr Lys Lys Ser Val Leu Glu465 470 475 480Thr Glu Arg Glu Ile Phe Ala Tyr Trp Arg Asp Gln Val Lys Lys Lys 485 490 495Gln Thr Gly Ile Cys 50029500PRTOryza sativa subsp. japonica 29Met Val Leu Thr His Val Glu Ala Val Glu Glu Gly Ser Glu Ala Ala1 5 10 15Ala Ala Val Phe Ala Ser Arg Tyr Val Gln Asp Pro Val Pro Arg Tyr 20 25 30Glu Leu Gly Glu Arg Ser Ile Ser Lys Asp Ala Ala Tyr Gln Ile Val 35 40 45His Asp Glu Leu Leu Leu Asp Ser Ser Pro Arg Leu Asn Leu Ala Ser 50 55 60Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Arg Leu Ile Leu Glu65 70 75 80Ala Ile Asn Lys Asn Tyr Ala Asp Met Asp Glu Tyr Pro Val Thr Thr 85 90 95Glu Leu Gln Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala 100 105 110Pro Val Gly Asp Gly Glu Lys Ala Val Gly Val Gly Thr Val Gly Ser 115 120 125Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln 130 135 140Asn Arg Arg Lys Ala Ala Gly Lys Pro His Asp Lys Pro Asn Ile Val145 150 155 160Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe 165 170 175Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Cys Tyr Val Met 180 185 190Asp Pro Val Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val 195 200 205Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Arg 210 215 220Arg Leu Asn Asp Leu Leu Ala Ala Lys Asn Lys Arg Thr Gly Trp Asp225 230 235 240Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe 245 250 255Ile Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser 260 265 270Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly 275 280 285Trp Val Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe 290 295 300His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe305 310 315 320Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Leu Arg 325 330 335Leu Gly Phe Glu Gly Tyr Lys Ser Val Met Lys Asn Cys Met Glu Ser 340 345 350Ala Arg Thr Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Thr Ile 355 360 365Ile Ser Lys Glu Glu Gly Val Pro Leu Val Ala Phe Thr Phe Lys Asp 370 375 380Gly Ala Gly Ala Gln Ala Phe Arg Leu Ser Ser Gly Leu Arg Arg Tyr385 390 395 400Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Ala Leu Glu His Met 405 410 415Thr Val Val Arg Val Val Val Arg Glu Asp Phe Gly Arg Pro Leu Ala 420 425 430Glu Arg Phe Leu Ser His Val Arg Met Ala Leu Asp Glu Met Asp Leu 435 440 445Ala Ala Arg Ala Pro Val Pro Arg Val Gln Leu Thr Ile Glu Leu Gly 450 455 460Pro Ala Arg Thr Ala Gly Glu Glu Ala Ser Ile Arg Val Val Lys Ser465 470 475 480Glu Ala Val Pro Val Arg Lys Ser Val Pro Leu Val Ala Gly Lys Thr 485 490 495Lys Gly Val Cys 50030496PRTNicotiana tabacum 30Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Gly Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Ile Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val

405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Ala Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 49531494PRTArabidopsis thaliana 31Met Val Leu Ala Thr Asn Ser Asp Ser Asp Glu His Leu His Ser Thr1 5 10 15Phe Ala Ser Arg Tyr Val Arg Ala Val Val Pro Arg Phe Lys Met Pro 20 25 30Asp His Cys Met Pro Lys Asp Ala Ala Tyr Gln Val Ile Asn Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Val Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Met Ile Ala Asn Leu Phe His Ala Pro Val Gly 100 105 110Glu Asp Glu Ala Ala Ile Gly Cys Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln His Arg Arg 130 135 140Lys Ala Gln Gly Leu Pro Ile Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Lys Leu Ser Glu Asp Tyr Tyr Val Met Asp Pro Ala 180 185 190Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Gln Leu Asn 210 215 220Asp Leu Leu Ala Glu Lys Asn Ala Glu Thr Gly Trp Glu Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr Pro 245 250 255Asp Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp Val Val 275 280 285Trp Arg Thr Lys Asp Asp Leu Pro Glu Glu Leu Val Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Met Asp Asn Ala Arg Arg 340 345 350Leu Arg Glu Gly Ile Glu Met Thr Gly Lys Phe Asn Ile Val Ser Lys 355 360 365Asp Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser Lys 370 375 380His Thr Val Phe Glu Ile Ala Glu Ser Leu Arg Lys Phe Gly Trp Ile385 390 395 400Ile Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Ala Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Gly Leu Ala Asp Arg Leu 420 425 430Ile Thr His Ile Ile Gln Val Leu Lys Glu Ile Glu Gly Leu Pro Ser 435 440 445Arg Ile Ala His Leu Ala Ala Ala Ala Ala Val Ser Gly Asp Asp Glu 450 455 460Glu Val Lys Val Lys Thr Ala Lys Met Ser Leu Glu Asp Ile Thr Lys465 470 475 480Tyr Trp Lys Arg Leu Val Glu His Lys Arg Asn Ile Val Cys 485 4903214PRTArtificial sequenceSynthetic peptide 32Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr1 5 103315PRTArtificial sequenceSynthetic peptide 33Val Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr1 5 10 15341704DNALolium perenne 34catctccacg gcaccacctt tcctcgagct cgcggccgat tcccttcgct gcgcgcggcc 60gacctgccgt cactgtctcc gtgcaagccg cgaccgcgac cgccagcaac catggttctc 120actgtggcag cgaccgccgc ggacacggcc gagccgctca actccacctt cttcgccacc 180cgctacgtcc gcgaccagct cccccggtac cggatgccgg agaactcgat ccccaaggag 240gcggcgtacc agatcatcag cgacgagctc atgctcgacg gcaacccgcg cctcaacctc 300gcatccttcg tcaccacccg gatggagccc gaggtcggca agctcatcat ggactccgtc 360aacaagaact acgtcgacat ggacgagtac cccgtcacca ccgagctcca gaaccgttgt 420gtaaatatga tagcccacct gttcaatgca ccgatcaagg aggaggagac agcaattgga 480gttgcgacag tgggatcctc agaagcaata atgcttgcag gcctggcctt caagaggaag 540tgggcaaata aaagaaagga ggaggggaag ccatatgaca aacctaacat tgttactggt 600gcaaatgttc aggtttgctg ggaaaaattt gctagatatt ttgaagtaga attgaaggag 660gtcaagttaa ctgaaggata ctatgtcatg gatcctttga aagctgttga aatggtggat 720gagaacacta tatgtgttgc agccatcttg ggatctactc tcactggaga gtatgaagat 780gttaaactat tgaatgacct gcttgtggaa aagaacaaga aaacagggtt taatgtgccg 840atccatgttg atgctgcaag tggaggattt atagcccctt ttcttcaccc tgagcttgag 900tgggacttca ggctaccatt ggtgaagagc atcaatgtta gtgggcacaa gtatggcctc 960gtctaccctg gtgttggatg ggtcatctgg cggagcaaag acgatttgcc tggagaactc 1020attttccata taaactacct gggaacagat cagcccacat tcacattgaa cttctccaaa 1080ggtgctagcc agatcattgc gcaatactat caactgatac gcctaggctt cgagggatat 1140aagcacatca tggagaattg ccaggccaat gcaaccgcgc tgagggaggg cttagaggca 1200accgggcgat tcgacatcct gtccaaagag gacggtgtgc ccctagtggc catccggctc 1260aaggacagct ccaaattcag cgtgttcgac atctccgaga acctgaggag gtttggctgg 1320attgtgcctg cctacaccat gcctgcggac gcagagcacg tggctgtcct ccgcgtcgtc 1380atcagggagg acttcaaccg cagcctctcc cagcggctcc tcgccgacat caacagggtc 1440gtgcaggagc tagatgccca cgcggtccat gccattaaga tgaccactgc tatcgcgaca 1500caaaccggcg agggtgctga ggatggcgtg gtgaccaaga agggcgttct ggacatcgag 1560aaggagttcg ccgcggcctg caaggacctg gtaaagaaca agaagactgg accctgctga 1620agggcatgcc ggcacagtac gcacgtacgt actatatgta tctatttttc ctggagtata 1680tctgaaccgt gaatacctgg ctcc 1704351455DNAOryza sativa 35atggcgctgt cgacggcgca gacaggggag tcgatgcact cctcgacgtt cgcgtcgcgg 60tacgtgcgca cggcgctgcc gaggttcagg atgccggaga agtcgatccc caaggacgcg 120gcgtaccaga tcatcaacga cgagctgatg ctcgacggca acccgcgcct gaacctggcg 180tccttcgtca ccacgtggat ggagcccgag tgcgacaagc tcatgatggc cgccatcaac 240aagaactacg tcgacatgga tgagtacccc gtcaccaccg agctccagaa ccggtgcgtg 300aacatgatcg cgcatctgtt caacgcgccg atcggggacg acgagacggc ggtcggggtg 360ggcacggtgg ggtcgtcgga ggccatcatg ctggcggggc tggcgttcaa gaggaagtgg 420cagaacagga tgaaggccga ggggaagccc cacgacaagc ccaacatcgt gacgggggcc 480aacgtgcagg tgtgctggga gaagttcgcg cgctacttcg aggtggagct caaggaggtg 540aagctgaccc aagggtacta cgtgatgaac ccggagaagg ccgtggagat ggtcgacgag 600aacaccatct gcgtcgccgc catcctcggc tccaccctca acggcgagtt cgaggacgtc 660aagatgctca acgacctcct caccgccaag aacgccgaga cagggtggaa cacgccgatc 720catgtggacg cggcgagcgg cgggttcatc gcgccgttca tctacccgga gctggagtgg 780gacttccggc tgccgctggt gaagagcatc aacgtcagcg gccacaagta cgggctcgtc 840tacgccggcg tcgggtgggt catctggcgc aacaaggagg acctccccga tgagctcatc 900ttccacatca actacctcgg cgccgaccag ccaaccttca cgctcaactt ctccaaagga 960tcgaaccaga taattgcgca gtattaccag ctcattcgtc tcggattcga ggggtacaag 1020gacatcatgc agaactgccg ggacaacgcg acggtgctcc gggaggggat cgagaagacg 1080ggccacttcg acgtggtgtc caaggactcc ggcgtgccgc tggtggcctt ctccctcaag 1140gactcgtcgc ggtacacggt gttcgaggtg gccgagagcc tccgccgctt cggctggatc 1200gtgccggcgt acaccatgcc cgccgacgct gagcacgtcg ccgtgatgcg cgtcgtcatc 1260cgcgaggact tcagccgcgg cctcgccgag cgcctcatca ccgacctcac caagacggtg 1320gccgatatgg acgcccacgc cgtcaagaag gccgccgccg agccggccaa gaagaccgtg 1380cgggagatag agaaggaggt gaccacctac tggcggagtt tcgtcgccag gaagaagagc 1440agcctcgtct gctga 1455361509DNAArabidopsis thaliana 36atggtgctct cccacgccgt atcggagtcg gacgtctccg tccactccac attcgcatca 60cgttacgtcc gtacttcact tcctaggttc aagatgccgg aaaactcgat tcctaaggaa 120gcggcgtatc agatcatcaa cgacgagctg atgcttgacg ggaatccacg gttgaactta 180gcctcctttg tgacgacatg gatggagcct gagtgtgata aactcatcat gtcctccatc 240aacaagaact atgttgacat ggacgagtac cccgtcacca ccgaacttca gaaccgatgt 300gtgaacatga ttgcacatct attcaatgca ccgttagaag aggcggagac cgccgtcgga 360gtaggaaccg ttggatcatc ggaggccata atgttggccg gtttggcctt caagcgtaaa 420tggcagaaca agcgcaaagc tgaaggcaaa cccgtcgata aacccaacat tgtcaccgga 480gccaatgttc aagtgtgttg ggagaaattc gctaggtact ttgaggttga acttaaggaa 540gtgaaattga gtgaaggata ctatgtgatg gaccctcaac aagctgttga tatggttgat 600gagaacacca tttgtgttgc ggccattctt ggttccactc ttaatggaga attcgaagat 660gttaaactct tgaacgatct cttggtcgaa aagaacaaag aaaccggatg ggatacacca 720atccacgtgg atgcggcaag tggaggattc attgcaccgt ttttgtatcc ggaattggaa 780tgggacttta gacttccctt ggtgaagagt atcaatgtga gtggtcacaa gtatggactt 840gtgtacgcag ggattggttg ggtgatctgg agaaacaaag aggatttgcc tgaggaactc 900atcttccata tcaattatct tggtgctgac caacccacct ttactctcaa tttctccaaa 960ggttcaagtc aagtcattgc tcaatactac caacttatcc gattgggcca cgagggttac 1020agaaatgtga tggagaattg cagagagaat atgatcgtcc taagggaagg acttgagaag 1080acagaaaggt tcaacatcgt ctcaaaggac gagggagtgc cacttgtcgc tttctccttg 1140aaagatagca gctgtcacac tgagttcgaa atctccgaca tgcttcgcag gtatggatgg 1200atagtgccgg cctacacaat gcctccaaat gcacaacaca tcactgttct tcgtgtggtt 1260atcagagaag atttctcgag aacactcgct gagagacttg tgatcgatat agagaaagtg 1320atgcgtgagc tcgatgagct tccttcgaga gtgattcaca aaatatcact tggacaagag 1380aagagtgaat ctaacagcga taacttgatg gtcacggtga agaagagcga tatcgacaag 1440cagagagata tcatcactgg ctggaagaag tttgtcgccg acaggaagaa gacgagtggt 1500atctgctaa 1509371732DNAOryza sativa 37acttcgattg gttccaccgc gcctaccgag tttcccttgc taggcagtag agagagtctg 60tgatcgagag agaagaggat cgagcagcta gcaagccggc gggcgccatg gtgctctcgc 120acgcgagctc cggccgggac gacgccgtgc gctgcacctt cgcgacgcgc tacgcctgcg 180agacgctgcc gcggttcagg atgccggagc agtcgatccc gagggaggcg gcgtaccaga 240tcatcaacga cgagctgatg ctggacggga acccgcggct gaacctggcg tccttcgtca 300ccacgtggat ggagcccgag tgcgacaagc tcatcatgga ctccgttaac aagaactacg 360tcgacatgga cgagtaccct gtcaccacgg agctccagaa ccgttgtgtg aatatgatag 420ctcacctgtt caatgcacca atcaaggagg atgaaacagc tattggagtt gggacggtgg 480gatcctcaga agcaattatg cttgcaggac tggcattcaa gaggaagtgg caaaacaaac 540ggaaggaaca ggggaagcca tgtgacaaac ccaacattgt tactggtgct aatgttcagg 600tttgctggga gaaatttgcc agatattttg aagtagaact gaaggaggtt aagctcagtg 660aaggatacta tgtcatggat cctgtaaagg ctgttgaaat ggtggatgag aacactatat 720gcgttgcggc catcttgggc tctactctca ctggagagtt tgaggatgtt aagttattga 780ataatctcct aacagaaaag aataaggaaa ctgggtggga tgtgccaatt catgttgatg 840cagcaagtgg aggatttata gcaccttttc tataccctga gcttgaatgg gacttcaggc 900taccactggt gaagagcatc aatgtcagtg ggcacaagta tggccttgtg tatccaggtg 960ttggttgggt catttggcga agcaaagagg atttgcctga agaactcatt ttccatataa 1020actatctggg gacagaccag ccgacgttca ctctgaactt ctccaaaggt tccagccaga 1080taatcgcaca gtactatcaa ctaatacgcc tgggattcga gggatacaag aacatcatgc 1140agaattgcat ggagaacaca gcaatactaa gggaaggcat agaggcgact ggtcgattcg 1200aaatcctctc caaggaggcc ggtgtgccct tggtggcgtt ctcgctcaag gacagcggca 1260ggtacaccgt gttcgacatc tccgagcacc tgaggaggtt cggctggatc gtgccggcgt 1320acaccatgcc ggccaacgcc gagcacgtcg ccgtcctccg cgtcgtcatc agggaggact 1380tcagccggag cctcgccgag cggctcgtct cggacatcgt caagatcctg cacgagctgg 1440acgcccattc ggcccaggtg ctgaagatct ccagcgccat cgcgaagcag caatcgggcg 1500acgatggcgt ggtcaccaag aagagcgtcc tggagaccga gagggagatc ttcgcgtact 1560ggagggacca ggtgaagaag aagcagaccg gaatctgcta gtgtggctct gtgagaaatg 1620cttgaataac gtggcatgct cgatttgtgc atgggatggc cggatcggat gggactgact 1680ggcggtgtac ggacatggct gcccttgttc ttatgttgaa ctgttgatgt ag 1732381730DNABrassica juncea 38gacacattct ttaactacaa acaaaaacat tacaaaccta taattcaaag ttcttataaa 60cttcgagtga attgaaagac gatggttttg tctaagacag cttctggaac tgatgtttcc 120gtccattcaa cttttgcttc tcgttatgtc cgcaactcgc tccctcgatt cgagatgcct 180gagaactcca tcccgaagga agcagcgtac cagatcatca acgatgagct aatgctcgac 240ggtaacccta ggctaaatct agcctccttc gtgactacgt ggatggagcc agagtgtgac 300aagctcatga tggaatctat caacaagaac tacgttgaca tggacgagta ccctgtcacc 360accgagcttc agaaccgatg tgtcaacatg attgcgcgtc tctttaacgc gccgctaggt 420gacggtgagg ctgcggttgg tgtcggcacc gtgggatcgt ctgaggcgat tatgttggcc 480gggttggctt ttaagagaca gtggcagaac aagcgtaagg cccaagggct tccttatgat 540aagcctaata tcgtaaccgg agctaatgtt caggtttgct gggagaaatt cgcaaggtat 600ttcgaggtgg aacttaagga agtgaagctg agagaaggat actacgtgat ggaccctgaa 660aaggcagtcg aaatggtaga cgagaacacc atttgtgtcg cagccatcct cggttcgacg 720ctaaccggag aattcgaaga cgttaagctc ctcaatgacc tcctagtcga gaaaaacaag 780caaaccggat gggatactgg gaatcacgtg gacgcagcaa gtggtgggtt tattgcaccg 840ttcttgtatc cggagctgga gtgggatttc cggttaccat tggttaagag cataaatgtt 900agtggccaca aatacggtct ggtttatgct ggaatcggtt gggttgtgtg gagaaccaaa 960tctgatttgc ctgatgaact tatcttccac atcaattatc ttggcgctga tcaacccacc 1020ttcactctca acttctccaa gggttcgagt caagtgattg ctcagtacta ccaactgatt 1080cgtcttggat tcgagggata tcgtaacgtg atggataatt gtcgtgaaaa catgatggtc 1140ctaagagaag gactagagaa aacgggacgt ttcaacattg tctccaaaga aaacggtgtt 1200ccgttagtgg cgttttctct aaaagacagt agccgccaca atgagttcga agtggcggaa 1260actctccgcc gctttggatg gatcgttccg gcctacacgg tgccagcgga tgcagaacat 1320gtcaccgtcc tccgagtggt gattcgagaa gatttctctc gaaccttagc tgagagattg 1380gttgcagact ttgagaaggt tcttcacgag ctcgatacac ttccggccag ggttcgcgcc 1440aagatggcta atggaaaagc taaagttgtt aaacagacgg aggaggagac gacgagggaa 1500gttacggcat attggaagaa gtttgtggag acaaagaaga ctaaccagaa caagatttgc 1560taataaagaa aaattaatga gttgatattt tgttgttttg tctctaatta agctactttt 1620aaatcctttt cttccatcct agtcggatca tttctggttt caaattatcc tttttttatg 1680atccgatgtt gatttttggt atttggaata aatttaaaca tatacgttta 1730391701DNABrassica juncea 39gaaattcttc aacaacaaac aataacatta catataatcc ctctctactc tttcttttgt 60tcattcaaag aagatggttt tgtctaagac agcttcggaa tctgatgttt caatccattc 120aacttttgct tctcgttacg tccgcacctc tctcccacga tttgagatgc ctgagaactc 180gatcccaaag gaagcagcgt accaaatcat caacgacgag ctaatgctcg acggtaaccc 240aaggctaaat ctagcctcct tcgtgaccac gtggatggag ccagagtgcg acaagctcat 300gatggaatcc atcaacaaga actacgtcga catggacgag taccctgtca ccaccgagct 360tcagaaccga tgcgtcaaca tgatcgcgcg tctcttcaac gcgccgctag gtgacggcga 420ggctgcggtt ggcgtcggca ccgtgggatc gtcggaggcg attatgttgg ctggattggc 480ctttaagaga cagtggcaga ataagcgtaa ggctcaaggg cttccttatg ataagcccaa 540tatcgttacc ggagccaatg ttcaggtttg ctgggagaag tttgcaaggt atttcgaggt 600ggagcttaaa gaagttaagc taagagaagg atactacgtg atggacccag agaaggcggt 660cgaaatggta gacgagaaca caatctgtgt tgcagccatc ctcggttcca ctctaacagg 720agaattcgaa gacgttaagc tccttaacga cctcctagtc gagaaaaaca agcaaaccgg 780atgggatacg gggatccatg tggacgcagc gagtggtggg tttattgctc ctttcttgta 840tccagagctg gagtgggatt tccggttacc attggttaag agcataaatg tgagtggtca 900caaatacggt ttggtttacg ctggaatcgg ttgggttgta tggagaacca aatccgattt 960gcctgatgaa cttatcttcc atatcaacta tcttggcgct gaccaaccga ccttcactct 1020caacttctcc aaaggttcaa gtcaagtgat tgctcagtac taccagctga ttcgtcttgg 1080attcgaggga tatcgcaacg tgatggataa ttgccgtgaa aatatgatgg tcctaagaga 1140aggattagag aagacaggac gtttcaacat agtctcaaaa gaaaacggtg ttccgttagt 1200ggcattttct ttaaaagaca gtagtcgcca cgacgagttc gaagtggccg agactctccg 1260tcgctttggg tggattgttc cggcctacac gatgcccgcg gatgctcaac atgtcaccgt 1320cctccgagtg gtgattcgag aagatttctc tcgaactttg gctgagagat tggtcgcaga 1380cttcgagaag gttctccacg agctcgatac gcttccggcg agggttcagg ccaagatggc 1440taacggaaac gctaacggtg ttaagaagac ggaagaggaa acgacgaggg aagttactgc 1500gtattggaag aagtttgtgg aagcaaagaa gagtaacaag aacaggattt gctaatgaaa 1560tttaatgaac taatgttctg ctgttttatc cctaataatg ctccatttga aacccttttc 1620ttccatgcta gctgctcgga ttactttggt ttcaaatttt ttttcatgat ttgatgttga 1680tgagttataa ttttcatgta c 1701401707DNABrassica juncea 40gatattcttc ttcctcctcc cactaccaaa aaagaaagaa agatggttct aagtcgagcg 60gccaccgaaa gtggcgaaaa tgtttgctcg acgttcggat ctcgctatgt ccgcaccgca 120ctgcccaagc ataagattgg tgagagctcg atcccgaagg aggctgcgta tcagatcata 180aaagatgagc tgatgcttga tggtaacccg aggctgaacc tggcttcgtt tgtgacgaca 240tggatggagc cagagtgtga caaactcatc atggaatcta tcaacaagaa ctacgtcgac 300atggacgagt accctgtcac taccgaactc cagaaccgat gtgtaaacat gatagctcgg 360ctgttcaatg cgccgcttga ggaaactgag accgccatgg gagtaggcac tgttgggtct 420tcggaagcca tcatgttagc cggattggcc ttcaaaagga attggcagaa caaacgcaaa 480gctgagggta aaccctatga caaacccaac attgtcaccg gagccaatgt tcaagtgtgc 540tgggagaaat tcgctaggta cttcgaggtg gagctaaaag aagtgaagct tagtgaaggt 600tactacgtga tggatccgga taaagcagct gaaatggtag acgagaatac aatctgtgtt 660gctgccatac ttggttctac actcaacggt gagttcgaag acgtcaagcg ccttaatgac 720ttgctggtca agaaaaacga agagactggc tggaacactc caatccacgt tgacgcagca 780agtggaggct tcatagctcc gtttatctac cctgagttgg aatgggactt taggcttcct 840ttggtgaaga gtatcaatgt gagtggtcat aagtatgggc tggtctatgc tggtattggc 900tgggtcgtgt ggaggacaca acaggatttg cctgatgagc tcatctttca tattaactat 960cttggtgctg atcaacccac atttactctc aatttctcca agggatcgag ccaaattatt 1020gctcaatatt atcagctcat

tcgtcttggc ttcgagggct acaagaacgt gatggagaac 1080tgcagagaga acatggtggt tctgagagaa gggatcgaga aaacagagcg tttcaacata 1140gtctcaaagg aggtaggagt tccactcgta gccttctccc tcaaggacca cagtttccac 1200aacgagttcg aaatctcaga gatgctacgc cgtttcggct ggattgtccc ggcttacaca 1260atgcctgcgg atgcgcaaca catcacagtt ctgcgtgttg tcatcaggga agatttctca 1320agaacacttg cggagagact tgtggctgat attgtgaagg tgcttcacga gctcgacacc 1380ttgccttcca agatatctaa gaagatggga gcagaggatt tcggaaacgt gaaagggaag 1440aaggtggata gggatgttct gatggaagtc attgttggat ggaggaagtt tgtgaaggac 1500aggaagaaga tgaatggtgt gtgttgatct gaagtgtctt gtggtttgtg tttgaatgta 1560tcgtcgtcta ataaataaaa tgaagtatgt gtgtgaggac ttgtggtttg atgaaagagt 1620tgtgtctggt atctatttga gacgataatt aattttggat tctgttgtat gcttgaagag 1680tcttttaaat aatattatca tattact 1707411485DNAArabidopsis thaliana 41atggtactcg caaccaactc tgactccgac gagcatttgc attccacttt tgcttctaga 60tatgtccgtg ctgttgttcc caggttcaag atgcctgacc attgcatgcc caaagatgct 120gcttatcaag tgatcaatga tgagttgatg cttgatggta atcccaggct taacctagcc 180tcctttgtca ccacttggat ggaacctgag tgtgacaaac tcatcatgga ttctgtcaat 240aagaactatg ttgatatgga tgaatatcct gtcaccactg agctccagaa ccggtgtgta 300aatatgatag caaacttgtt ccatgctccc gttggagaag acgaggctgc tattgggtgt 360ggaactgttg gttcatctga ggctataatg cttgctggtt tggctttcaa aaggaaatgg 420caacatagga gaaaagctca gggtctacct attgataagc ctaacattgt cactggagcc 480aatgttcagg tgtgctggga gaagtttgca aggtactttg aggtagagct caaagaggtg 540aaactaagtg aagactacta tgttatggat ccagctaaag ctgtagagat ggtggatgag 600aataccatct gtgttgcagc aattctagga tccacactta ctggagagtt tgaggacgtt 660aagcaattga acgatctctt agctgagaaa aacgcagaga caggatggga aactcctatt 720catgttgatg cagccagtgg aggattcatt gctcctttcc tctaccctga tcttgaatgg 780gactttaggc ttccatgggt gaagagtatt aacgtcagtg gtcacaagta tggacttgtg 840tatgcaggag ttggttgggt tgtctggaga acaaaagatg atttgccaga ggaacttgtc 900ttccacatca actacttggg agctgatcaa cccactttca ctctcaactt ctcaaaaggg 960tcgagccaaa tcattgctca gtactatcag tttatccgac taggctttga gggatacaag 1020aacataatgg aaaactgcat ggataacgca aggaggctaa gagaaggaat agagatgaca 1080gggaagttca acattgtgtc caaagatatt ggcgtgccac tagtggcatt ctctctcaaa 1140gacagtagca agcacacggt gtttgagatc gcagagtctt tgagaaaatt cgggtggatc 1200ataccggctt acactatgcc tgcagatgca cagcacattg ctgtgctcag agttgtgata 1260agagaagact ttagccgagg ccttgcagat agactcatca cacatatcat tcaggtgctg 1320aaagagattg aagggcttcc tagcaggatt gcacatcttg ctgcggctgc agcggttagt 1380ggtgatgatg aagaagttaa agtgaagact gccaagatgt ccttggagga tatcactaag 1440tattggaaac gccttgtgga acacaagaga aatattgtct gctaa 1485421482DNAArabidopsis thaliana 42atggttttgt ctaagacagt ttccgaatct gatgtctcaa tccattcaac ttttgcttct 60cgttacgtcc gcaactctct tccacgattc gaaatgcctg agaactcaat cccaaaagaa 120gcagcttacc aaatcatcaa cgacgagcta atgctcgatg gtaacccaag gctgaaccta 180gcttccttcg tgaccacatg gatggagcca gaatgtgaca agctcatgat ggagtccatc 240aacaagaact acgtcgacat ggacgagtac cctgtcacca ctgagcttca gaaccgatgt 300gttaacatga tagcacgtct cttcaacgcg ccgcttggtg acggtgaagc tgccgttggt 360gttggcaccg tcggatcgtc ggaggcgatt atgttggccg gtttggcttt taagagacaa 420tggcagaata agcgtaaggc ccaagggctt ccttatgata agcccaatat cgtaaccggt 480gctaatgtcc aggtttgctg ggagaaattc gcaaggtatt tcgaagtgga gcttaaggaa 540gtgaacctaa gagaagacta ttacgtgatg gaccctgtaa aggcggtcga aatggtagac 600gaaaacacaa tttgtgtcgc tgccatcctc ggttcaacgt taaccggtga attcgaagac 660gttaagctcc tcaacgacct ccttgtcgag aaaaacaagc aaaccggatg ggacacgcca 720atacacgtgg acgcagcgag tggtgggttt attgctccgt tcttgtatcc ggagctggag 780tgggatttcc ggctaccgtt ggttaagagt attaatgtga gtggtcacaa atacggtttg 840gtttacgccg gtattggttg ggttgtatgg agaaccaaaa ccgatttgcc tgatgaactt 900atcttccata tcaattatct tggcgctgat caaccaacct ttacactcaa cttctccaaa 960ggttcaagtc aagtgattgc tcagtactac cagctgattc gtcttggatt cgagggttat 1020cgcaatgtga tggataattg tcgggaaaac atgatggtac taagacaagg attagagaaa 1080acgggacgtt ttaaaatcgt ctccaaagaa aacggtgttc cgttagtggc gttttctctc 1140aaagatagta gccgccacaa cgagttcgag gtggcccata cactccgtcg cttcggctgg 1200atcgttccgg cctacacgat gcctgcggat gcgcagcatg tcactgtcct tcgagttgtt 1260atccgagaag atttctctcg aaccttagcc gagagattgg tagctgattt cgagaaggtt 1320ctacacgagc tcgatacgct tccggcgagg gttcacgcca agatggctaa tggaaaagtt 1380aacggtgtta agaagacgcc agaggagacg cagagagaag tcacggccta ctggaagaag 1440ttgttggaga ctaagaagac caacaagaac acaatttgct aa 1482431712DNAArabidopsis thaliana 43acatattatt caactacaaa acaaaaacat tgcacatttt tccctttact tttctttagc 60tcatttcaag aagatggttt tatctaagac agcttccaaa tccgatgatt caatccattc 120aacttttgct tcccgttatg tccgcaactc tatctcacga ttcgaaatac ctaagaactc 180gatccctaag gaagcagcat accaaatcat caacgacgag ctcaagtttg acggtaaccc 240gaggctaaac ctggcctcct ttgtgaccac ttggatggag ccagaatgtg acaagctcat 300gatggaatcc atcaacaaga acaacgttga gatggaccaa taccctgtta ccaccgacct 360tcagaatcga tgcgttaaca tgattgcgcg tctcttcaac gcgcctttag gtgacggtga 420agccgccatt ggtgttggca cggtggggtc atcggaggca gtgatgttgg ccggactggc 480ctttaagaga cagtggcaga acaagcgtaa ggccctaggg ctgccttatg atagacctaa 540tattgtaacc ggagccaata ttcaggtttg cttggagaaa tttgcaaggt attttgaagt 600ggagcttaag gaagtgaagc tgagagaagg atattacgtg atggaccctg acaaagcggt 660tgaaatggta gacgaaaaca ctatatgcgt cgtggccatc ctcggttcga cactaaccgg 720agaattcgaa gacgttaagc tcctcaacga ccttttagtc gagaaaaaca agaaaaccgg 780atgggatacg ccgattcacg tggacgcagc gagtggtggg tttattgctc ccttcttgta 840tccggacttg gagtgggatt tccggttacc gttggttaag agcataaatg tgagtggtca 900caaatacggt ttggtttacg ccggtatcgg ttgggtcgta tggagaacca aaaccgattt 960gcctgatgaa cttatcttcc atatcaatta tcttggagct gatcaaccca catttaccct 1020caacttctct aaagggtcaa gtcaagtgat tgctcagtac taccagttga ttcgtcttgg 1080attcgaggga tatcgcaacg tgatggataa ttgccgcgag aacatgatgg tactaagaca 1140aggattagag aaaacgggac gttttaacat cgtctccaaa gaaaacggtg ttccgttagt 1200ggcgttttct ctcaaagata gtagccgcca caacgagttc gaggtggccg aaatgcttcg 1260tcgcttcggc tggatcgttc cggcctacac gatgcctgcg gatgcgcaac atgtcacggt 1320ccttcgagtt gttatccgag aagatttctc tcgaacctta gctgagagat tggtagccga 1380tttcgagaag gttctacacg agctcgatac gcttcccgcg agggttcacg ccaagatggc 1440tagtggaaaa gttaacggtg ttaagaagac gccagaggag acgcaaagag aagtcacggc 1500ctactggaag aagtttgtgg acactaagac tgacaagaac ggcgttccgt tagtagcaag 1560tattaccaat caatgatggg ccaaatgtat attcaaatta ttgggtgcag acaaattttt 1620ttttagctaa aggcccaact acaccatagc aaatcagcac cattcgatct cagaccattc 1680aaacactgta ataaaaacat ttttagtttt ac 1712441776DNANicotiana tabacummisc_feature(1616)..(1616)n is a, c, g, or t 44aaaattaatt ctcctccttt ttctctgatc ttttgtacca aggtttaacc tcattctttc 60tagttttcct tgttcatttt ttcccaaaat ggttctctcg aaaacctcct ctgagtcgga 120cgtttcggta cactccactt ttgcctctcg ctatgttcga acttcccttc caaggtttga 180gatggcggag aattcgatac caaaagaggc ggcatttcaa ataattaacg acgagttgat 240gcttgacggg aatccaaggc tgaacttggc ttcatttgtg acaacatgga tggagccaga 300gtgtgataag cttatgatgg actccattaa caagaactat gttgacatgg atgaataccc 360tgttaccact gagcttcaga atcgctgcgt gaacatgata gcacgtttat tcaacgcgcc 420actagaagag aaggagacag cagttggagt gggtacagtt ggttcatcgg aggccataat 480gctagcgggg cttgcattca agagaaattg gcaaaacaaa cgcaaagctg agggcaaacc 540ttacaataag cccaacattg tcactggcgc caatgttcag gtgtgctggg agaaatttgc 600caactatttt gaagtggaat tgaaagaagt aaagctaagg gaagggtact atgtgatgga 660cccagtccag gctgtggaga tggttgatga gaacaccatt tgtgttgctg caatcttggg 720ttcaaccctt aatggagaat ttgaagatgt caagctcttg aatgatcttt tgattgaaaa 780gaacaagcaa actggatgga acacaccaat tcatgtggat gcagcaagtg gtggattcat 840tgcaccattc ctgtacccag agctggagtg ggactttagg cttcccttag tgaagagcat 900aaatgtgagt gggcacaaat atgggcttgt ctatgctggt attggttggg ttatttggag 960gaccaaacaa gatttgcctg aagaactcat tttccacatc aactatcttg gagctgatca 1020gcccaccttt actctcaatt tctccaaagg ttcaagtcaa gtcattgctc aatattatca 1080gctaatccgt ttgggctatg aggggtaccg aaatgtaatg gagaactgtc gcgaaaatgc 1140cattgtgcta agagaaggac tcgaaaaaac aggacgtttt aacatagtct ccaaagatga 1200aggtgtccct ttggtggcct tttccctcaa ggacaatagc cgtcacaatg agttcgaggt 1260gtccgagacg ctccgtaggt tcgggtggat cgtcccggcc tacacgatgc ccgctgacgc 1320ccaacacgtc acggtgcttc gtgtggtgat ccgggaggac ttctcgcgaa ccctagcaga 1380gcgtctcgtc ctcgacattg tcaaggtcct ccacgagctg gacacacttc cagctaggct 1440gagcgccaaa ttagaggagg tgaagctggt caagaatgga aagaaatttg aacttgaagt 1500tcaaagggaa gttaccaatt attggaagaa gtttgtttta gctaggaaag cacctgtttg 1560ctagagaagg atttttgaag gaattagtaa caaaactagg attttccttt tctttnattt 1620tttgggggtt atctttcctc ctaaattttg tngaaaaaca aacgaaattt tcataagaat 1680gatgacggta tcaaaatttg aatgtaatgg aactttttnt tggaagttng tctttttgta 1740aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 1776451672DNANicotiana tabacummisc_feature(1550)..(1550)n is a, c, g, or t 45gccatggttc tgtccaagac agcgtcggaa agtgacgtct ccatccactc cactttcgct 60tcccgatatg ttcgaacttc tcttcccagg tttaagatgc cagagaattc tataccaaaa 120gaagcagcat atcaaatcat aaatgatgag cttatgttag atggaaatcc aaggctaaat 180ttagcatctt ttgtgacaac atggatggaa ccagagtgta ataagttaat gatggattcc 240attaacaaga actacgttga catgggtgaa taccctgtaa ccactgagct tcagaatcga 300tgtgtaaata tgatagctca tttgtttaac gcaccacttg gagatggaga gactgcagtt 360ggagttggaa ctgttggatc ctctgaggct attatgcttg ctggattagc cttcaagaga 420aaatggcaaa ataaaatgaa agcccaaggg aagccctgcg acaagcccaa tattgtcact 480ggtgccaatg tccaggtgtg ttgggagaaa tttgcaaggt attttgaagt ggagttgaaa 540gaagtaaaat tgagtgatgg atactatgtg atggaccctg agaaagctgt ggaaatggtg 600gatgagaata caatttgtgt agctgctatc ttgggttcca ctctcaatgg tgaatttgaa 660gatgttaagc gcttgaatga cctcttgatt gagaagaaca aagaaaccgg gtgggacact 720ccaattcatg tggatgcagc aagtggtgga ttcattgcac cattccttta tcctgagctt 780gaatgggatt ttagattacc attggtgaag agtattaatg tgagtggtca caaatatggt 840cttgtctatg ctggtattgg ttgggccatt tggaggaata aggaagactt gcctgatgaa 900cttattttcc acattaatta tcttggtgct gatcaaccta ctttcactct caacttctct 960aaaggttcta gccaagtaat tgctcaatat taccaactta ttcgcttggg ttttgagggt 1020tacaagaatg ttatggagaa ttgtcaagaa aatgcaaggg tactaagaga aggacttgaa 1080aaaagtggaa gattcaatat aatctccaaa gaaattggag ttccattagt agctttctct 1140cttaaagaca acagtcaaca caatgagttc gaaatttctg aaactcttag aagatttgga 1200tggattattc ctgcatatac tatgccacca aatgctcaac atgtcacagt tctcagagtt 1260gttattagag aagatttctc ccgtacactc gcggagcgac tggtgataga cattgaaaaa 1320gtcctccacg agctagacac acttccggcg agggtcaacg ctaagctcgc cgtggccgag 1380gcgaatggca gcggcgtgca taagaaaaca gatagagaag tgcagctgga gattactgct 1440gcatggaaga aatttgttgc tgataagaag aagaagacta atggagtttg ttaatttaat 1500ttacaaaata tgttataata tgatgattta tgactactag caatattggn attgctgttt 1560aaatttgaat tgttgggnct ttgggtanac ttgaggagct anctatttat tgcaaggcaa 1620aactgggtga ttttgggcaa aataatgggn tnataaaccc aattttcttg tg 1672461597DNAGlycine max 46tgggaaccca aggttgaatt tggcatcatt tgtgacgact tggatggagc cagagtgtga 60taaactcatc atggctgcca ttaataagaa ctatgttgac atggacgagt accctgtcac 120cactgagcta cagaatcgat gtgttaacat gatagctcat cttttcaatg caccactaga 180agagactgag gcagcagttg gtgttggcac ggttggctca tcagaggcca ttatgttggc 240tggattggca ttcaaaagaa agtggcaaaa cagaaggaaa caagagggaa agccttatga 300caaacccaac attgtcactg gagccaacgt tcaggtttgc tgggagaaat ttgcaaggta 360ctttgaggtg gagttaaagg aggtgaagct ccgtgatgat tattatgtaa tggaccctga 420aaaggccgtg gaattggtgg atgagaacac tatttgtgtt gctgctatcc ttggttccac 480actaaatgga gagtttgaag atgtcaaacg cttaaatgat ctcctaattg aaaagaacaa 540aataactggg tgggacactc ctattcatgt tgatgcagcc agcggtgggt tcatagcccc 600atttatttac ccagagcttg agtgggactt ccggttacaa ctagtgaaga gcatcaatgt 660tagtgggcac aagtatggtt tggtctatgc tggaatcggt tgggttatct ggagaagcaa 720gcaggacttg cctgaggaac tcatctttca catcaactat cttggggctg atcaacccac 780cttcaccctt aacttctcca aaggttctag ccaagtcatt gctcaatact accaactgat 840tcgccttggt tttgagggat atagaaacgt gatggaaaac tgcagggaca acatgctggt 900gctgaaagag ggactcgaga aaacagggcg attttcaatt gtgtccaaag acaatggtgt 960gcctttggtg gctttcacac tgaaagacca cacccacttt gacgaattcc aaatctcaga 1020ctttttaagg cgctttgggt ggatagtgcc agcatacacc atgcccccag atgctcaaca 1080tgtcacagtg cttcgtgttg tcatcaggga ggacttctca aggaccctcg cggagcgtct 1140cgtgtccgat gtggagaagg tgctgcatga gcttgattca cttcctgcaa gggtcatcag 1200cagcaccact gtgacactca gtgcagaaga aaatggcaag gtagtggttg ctaagaagaa 1260tcctatggag actcagaggg aaatcactgc catttggaag aagtttgtgt tggagaggaa 1320gaagaacaat gacaagatga atggtgtttg ttagtgttag cttcaagggg gtgttgtgcc 1380tatgcctctg ccttattgta cttctttatt atatatataa tttgggcagt aagtactttt 1440gattgagtca agctatttat ctgtttcagt tgtttattga tgagaggcaa atattaaatt 1500gtgttttcgt ttgtacaagt agtgattcac tgtattttga ctattttctc tgaatcatga 1560attatattta tctcccctaa aaaaaaaaaa aaaaaaa 1597471745DNANicotiana tabacum 47tctagatcgt actaccacca ctacgccgcc atggttctgt ccaagacagc gtcggaaagt 60gacgtctcca tccactccac tttcgcttcc cgatatgttc gaacttctct tcccaggttt 120aagatgccag agaattcaat accaaaggaa gcagcatatc agattataaa tgatgagctt 180atgttagatg gaaatccaag gctaaattta gcatctttcg ttacaacatg gatggagcca 240gaatgtaata cgttaatgat ggattccatt aacaagaact acgttgacat ggatgaatac 300cctgtaacca ctgagcttca gaatcgatgt gtaaatatga tagctcattt gtttaatgca 360ccacttggag atggagagac tgcagttgga gttggaactg ttggatcctc tgaagctatt 420atgcttgctg gattagcctt taaaagaaaa tggcaaaata aaatgaaagc ccaaggcaag 480ccctttgata agcccaatat cgtcaccggt gctaatgtcc aggtgtgttg ggagaaattt 540gcaaggtatt ttgaagtgga gttgaaagaa gtaaaattga gtgatggata ctatgtgatg 600gaccctgaga aagctgtgga aatggtggat gagaatacca tttgtgttgc tgctatctta 660ggttcaacac tcaatggtga atttgaagat gttaagcgtt tgaatgacct tttgattgag 720aagaacaaag aaaccgggtg ggacactcca attcatgtgg atgcagcaag tggtggattt 780attgcaccat tcctttatcc agagcttgaa tgggacttta gattgccatt ggtgaagagt 840attaatgtga gtggtcacaa atatggtctt gtctatgctg gtattggttg ggccatttgg 900aggaataagg aagacttgcc tgatgaactt attttccaca tcaattacct tggtgctgat 960caacctactt tcactctcaa cttctctaaa ggttctagcc aagtaattgc tcaatattac 1020caacttattc gcttgggttt tgagggttac aagaatgtta tggagaattg tcaagaaaat 1080gcaagggtat taagagaagg aattgaaaaa agtggaagat tcaacataat ctccaaagaa 1140attggagttc ccttagtagc attttctctt aaagacaaca gtcaacacaa tgagttcgaa 1200atttctgaaa ctcttagaag atttggatgg attgttcctg catatactat gccaccaaat 1260gctcaacatg ttacagttct cagagttgtc attagagaag atttctcccg cacactagcg 1320gagcgactgg taatagacat tgaaaaagtc ctccacgagc tagacacact tccggcgagg 1380gtcaacgcta agctagccgt ggccgaggcg aatggcagcg gcgtgcataa gaaaacagat 1440agagaagtgc agctagagat tactactgca tggaagaaat ttgttgctga taagaagaag 1500aagactaatg gagtttgtta atttaattta acaaaaaaaa agtttataat atggtgattt 1560atgtaactac tagcagtcgt actgcttgtt ttttatattt gagttgatgt gttttttgag 1620cacttgagga gctagctagt tattgctagt gaaaaattgg atgatatatt ttggactact 1680ttgtaagttt gtattattaa tccaaattaa acgatattta tcatgcaaaa aaaaaaaaaa 1740aaaaa 1745481605DNAArabidopsis thaliana 48atatttttat gttcaacaat tcagcataac tcaaacacaa tggtactcgc aaccaactct 60gactccgacg agcatttgca ttccactttt gcttctagat atgtccgtgc tgttgttccc 120aggttcaaga tgcctgacca ttgcatgccc aaagatgctg cttatcaagt gatcaatgat 180gagttgatgc ttgatggtaa tcccaggctt aacctagcct cctttgtcac cacttggatg 240gaacctgagt gtgacaaact catcatggat tctgtcaata agaactatgt tgatatggat 300gaatatcctg tcaccactga gctccagaac cggtgtgtaa atatgatagc aaactttttc 360catgctcccg ttggagaaga cgaggctgct attgggtgtg gaactgttgg ttcatctgag 420gctataatgc ttgctggttt ggctttcaaa aggaaatggc aacataggag aaaagctcag 480ggtctaccta ttgataagcc taacattgtc actggagcca atgttcaggt gtgctgggag 540aagtttgcaa ggtactttga ggtagagctc aaagaggtga aactaagtga agactactat 600gttatggatc cagctaaagc tgtagagatg gtggatgaga ataccatctg tgttgcagca 660attctaggat ctacacttac tggagagttt gaggacgtta agcaattgaa cgatctctta 720gctgagaaaa acgcagagac aggatgggaa actcctattc atgttgatgc agccagtgga 780ggattcattg ctcctttcct ctaccctgat cttgaatggg actttaggct tccatgggtg 840aagagtatta acgtcagtgg tcacaagtat ggacttgtgt atgcaggagt tggttgggtt 900gtctggagaa caaaagatga tttgccagag gaacttgtct tccacatcaa ctacttggga 960gctgatcaac ccactttcac tctcaacttc tcaaaagggt cgagccaaat cattgctcag 1020tactatcagt ttatccgact aggctttgag ggatacaaga acataatgga aaactgcatg 1080gataacgcaa ggaggctaag agaaggaata gagatgacag ggaagttcaa cattgtgtcc 1140aaagatattg gcgtgccact agtggcattc tctctcaaag acagtagcaa gcacacggtg 1200tttgagatcg cagagtcttt gagaaaattc gggtggatca taccggctta cactatgcct 1260gcagatgcac agcacattgc tgtgctcaga gttgtgataa gagaagactt tagccgaggc 1320cttgcagata gactcatcac acatatcatt caggtgctga aagagattga agggcttcct 1380agcaggattg cacatcttgc tgcggctgca gcggttagtg gtgatgatga agaagttaaa 1440gtgaagactg ccaagatgtc cttggaggat atcactaagt attggaaacg ccttgtggaa 1500cacaagagaa atattgtctg ctaagcgcaa gtcttattct ttgcacttat atggtaataa 1560ggttttgatt tagaccattt ggcggtaata aggttttaat ttttt 1605492441DNAOryza sativa 49tgatagctcg gcaggcaaga tggtgctctc caaggccgtc tccgagagtg acatgtccgt 60gcactccacc ttcgcctccc gctacgtccg cgcctccctc ccaaggtacg tgctctcgat 120ctgctctctc tctgttcttg ctgttcttgg cccaatgcag agcttcttcc aatatttcat 180ggctgactcg atgggattga tcgattggtg ttattaggta ccggatgccg gagaactcga 240tcccgaagga ggcggcgtac cagatcatca acgacgagct gatgctggac ggcaacccgc 300ggctgaacct ggcgtcgttc gtcaccacgt ggatggagcc cgagtgcgac aagctcatca 360tggccgccat caacaagaac tacgtcgaca tggacgagta ccccgtcacc accgagctcc 420aggtacagca ccctgcaggc atctctgctt cgttgaattt tctcctatca cctcgcgttt 480tctcgccgcc gcgcgcggct cgaatgcaca ctggaaatcc ccggattttt ggtcgataaa 540accggcttgg ccagttggcc tggcctggca gattggtgat aagaatctca ggctagaaac 600tttatttccg agtttatcat ttgcgtcatt cacgggaggc agaatggggg aagaacatgg 660cacagtttcc gagatgatca ccaattcgca ttagaaaaaa tatataggaa gtccaataaa 720taaagtaagg ggatgggaag aatgatcacc atatctcttc atttttcgca ttaaaatatg 780gaaggtgcat

gaatgaaggg aaatgggaaa gaataatgcc tggtttggtc aaaacagggc 840aagagaccaa agttccatct actgaaacgg cgtggacaac agtcaaaacc tggaacacag 900aagacaccac tgcaactttc ccctttttgc gaatgagaca cattattagt ccttttttca 960gccagacatg ctttgggcca gatgatgtcc tagcatgaac tgtacaacat tttttttggg 1020ccgggccgtt ctacagctaa ttaacgaacg ctttggatgt gtgacgctgt gcagaaccgg 1080tgcgtgaaca tgatcgcgca cctgttccac gcgccgctcg gggaggacga gacggcggtg 1140ggcgtgggca cggtgggttc gtcggaggcc atcatgctgg ccgggctggc cttcaagcgg 1200cggtggcaga acaagcgcaa ggccgagggg aagccgttcg acaagcccaa catcatcacc 1260ggcgccaacg tgcaggtgtg ctgggagaag ttcgcccgct acttcgaggt ggagctcaag 1320gaggtgaagc tccgcgacgg ctactacgtc atggaccccg agaaggccgt cgacatggtc 1380aacgagaaca ccatctgcgt cgccgccatc ctcggctcca ccctcaacgg cgagttcgag 1440gacgtcaagc tactcaacga cctcctcgac aagaagaaca aggagactgg gtaactaaaa 1500ttactatttc tactacacta aattatctat ctatgaaagt gagattaatc gatgtcattg 1560cggccgtggt gtgcaggtgg gagacgccga tccacgtgga cgcggcgagc ggcgggttca 1620tcgcgccgtt cctgtacccg gagctggagt gggacttccg gctgccgtgg gtgaagagca 1680tcaacgtgag cggtcacaag tacgggctcg tctacgccgg catcggctgg tgcatctggc 1740gcaacaagga ggacctgccc gaggagctca tcttccacat caactacctc ggcaccgacc 1800agccaacctt caccctcaac ttctccaagg gctccagcca ggtcatcgcc cagtactacc 1860agctcatccg ccacggcttc gaggtataat caatggaaca caattaactc tcttgatcag 1920atgaattttt tttagctgat ctgacacacc catcgatccg atgttatttg caggggtaca 1980ggaacatcat ggagaactgc cacgagaacg cgatggtgct gaaggaaggg ctggtgaaga 2040cggggaggtt cgacatcgtg tccaaggacg aaggggtgcc gctggtggcg ttctcgctca 2100aggaccggag ccggcacgac gagttcgaga tctccgacat gctgcgccgc ttcggctgga 2160tcgtgccggc gtacaccatg ccgcccgacg cccagcacgt cacggtgctc cgcgtggtca 2220tccgggagga gttcagccgc accctcgccg agcgcctcgt cctcgacatc gagaaggtga 2280tgtaccagct cgacgcgctc ccctccaggc tcatgccccc cgtgccgccg gcgccgctgc 2340tggtggtcgc caagaagtcg gagctcgaga cgcagcggtc ggtgacggag gcgtggaaga 2400agttcgtgct cgccaagagg accaacggcg tctgctagtc t 2441501650DNAArabidopsis thaliana 50ctcagattct ctttcactaa acagaaacaa agatggtttt gacaaaaacc gcaacgaatg 60atgaatctgt ctgcaccatg ttcggatctc gctatgttcg cactacactt cccaagtatg 120agattggtga gaattcgata ccgaaagacg ctgcgtatca gatcataaaa gatgagctga 180tgcttgatgg taacccaagg cttaacctag cttcttttgt gactacatgg atggaaccta 240gtgtgacaaa ctcatcatgg actctatcaa taagaactac gttgatatgg atgagtaccc 300tgtcacaact gagctccaga accgatgtgt aaacattata gctcgactgt tcaatgcgcc 360actcgaggaa tctgagacgg cggtgggagt agggacagtt ggttcttcag aagccatcat 420gttagccgga ttggccttca aaagaaaatg gcagaacaaa cgcaaggctg agggtaaacc 480ctatgacaaa cccaacattg tcaccggagc caatgttcaa gtttgctggg agaaattcgc 540tcggtacttc gaggtggagc taaaggaagt aaacctaagt gaaggttact acgtgatgga 600tccagacaaa gcagcagaaa tggtagacga gaacacaatc tgtgtcgcag ccatattggg 660atccacactc aacggtgagt tcgaagacgt gaaacgtctc aatgacttgc tagtcaagaa 720aaacgaggag actggttgga acacaccgat ccacgtggat gcagcaagtg gagggttcat 780agctccgttt atctatcctg aattagaatg ggactttagg cttcctttgg ttaagagcat 840caacgtgagt ggtcacaagt atggactagt ctatgctggt attggttggg tcgtgtggag 900ggcagcagag gatttacctg aagagcttat ctttcatatt aattatcttg gtgctgatca 960acccactttc actctcaatt tctccaaggg atcgagccaa attattgctc aatactacca 1020gctcattcgt cttggattcg aggggtacaa aaatgtgatg gagaattgca tagagaacat 1080ggtggttctc aaagaaggta tagagaaaac agagcgtttc aacatagtct caaaggacca 1140aggagtgcca gtcgtcgcct tctctctcaa ggaccatagt ttccacaacg agttcgagat 1200ctctgagatg ctacgtcgtt ttggctggat cgtcccagct tacactatgc ctgccgatgc 1260acagcacatc acggttctgc gtgttgtcat cagggaagat ttctcaagaa cactcgcgga 1320gagacttgtt gctgatattt cgaaggtgct tcatgagcta gataccttgc cttccaagat 1380atctaagaag atgggaatag aagggatcgc gaaaaatgta aaggagaaga agatggagaa 1440ggagattctg atggaagtta ttgttggatg gaggaagttt gtgaaggaga ggaagaagat 1500gaatggtgtg tgctaagcaa gtgtgttgcc tttgtgtgga aatgaagagg tacttgcgag 1560gactttgcgt ttatcagttt atgtgtttgt atatctattt gatccagtta ttatggatta 1620tatacgcttg aaactcattt taagccattg 1650511506DNAOryza sativa 51atggtggtga gcgtggccgc gaccgactcg gacacggccc agccggtgca gtactccacc 60ttcttcgcct cccgctacgt ccgcgacccg ctcccgcggt tcaggatgcc ggagcagtcg 120atcccgaggg aggcggcgta ccagatcatc aacgacgagc tgatgctgga cgggaacccg 180cggctgaacc tggcgtcctt cgtcaccacg tggatggagc ccgagtgcga caagctcatc 240atggactccg ttaacaagaa ctacgtcgac atggacgagt accctgtcac cacggagctc 300cagaaccgtt gtgtgaatat gatagctcac ctgttcaatg caccaatcaa ggaggatgaa 360acagctattg gagttgggac ggtgggatcc tcagaagcaa ttatgcttgc aggactggca 420ttcaagagga agtggcaaaa caaacggaag gaacagggga agccatgtga caaacccaac 480attgttactg gtgctaatgt tcaggtttgc tgggagaaat ttgccagata ttttgaagta 540gaactgaagg aggttaagct cagtgaagga tactatgtca tggatcctgt aaaggctgtt 600gaaatggtgg atgagaacac tatatgcgtt gcggccatct tgggctctac tctcactgga 660gagtttgagg atgttaagtt attgaataat ctcctaacag aaaagaataa ggaaactggg 720tgggatgtgc caattcatgt tgatgcagca agtggaggat ttatagcacc ttttctatac 780cctgagcttg aatgggactt caggctacca ctggtgaaga gcatcaatgt cagtgggcac 840aagtatggcc ttgtgtatcc aggtgttggt tgggtcattt ggcgaagcaa agaggatttg 900cctgaagaac tcattttcca tataaactat ctggggacag accagccgac gttcactctg 960aacttctcca aaggttccag ccagataatc gcacagtact atcaactaat acgcctggga 1020ttcgagggat acaagaacat catgcagaat tgcatggaga acacagcaat actaagggaa 1080ggcatagagg cgactggtcg attcgaaatc ctctccaagg aggccggtgt gcccttggtg 1140gcgttctcgc tcaaggacag cggcaggtac accgtgttcg acatctccga gcacctgagg 1200aggttcggct ggatcgtgcc ggcgtacacc atgccggcca acgccgagca cgtcgccgtc 1260ctccgcgtcg tcatcaggga ggacttcagc cggagcctcg ccgagcggct cgtctcggac 1320atcgtcaaga tcctgcacga gctggacgcc cattcggccc aggtgctgaa gatctccagc 1380gccatcgcga agcagcaatc gggcgacgat ggcgtggtca ccaagaagag cgtcctggag 1440accgagaggg agatcttcgc gtactggagg gaccaggtga agaagaagca gaccggaatc 1500tgctag 1506521685DNAArabidopsis thaliana 52agattctctt tcactaaaca gaaacaaaga tggttttgac aaaaaccgca acgaatgatg 60aatctgtctg caccatgttc ggatctcgct atgttcgcac tacacttccc aagtatgaga 120ttggtgagaa ttcgataccg aaagacgctg cgtatcagat cataaaagat gagctgatgc 180ttgatggtaa cccaaggctt aacctagctt cttttgtgac tacatggatg gaaccagagt 240gtgacaaact catcatggac tctatcaata agaactacgt tgatatggat gagtaccctg 300tcacaactga gctccagaac cgatgtgtaa acattatagc tcgactgttc aatgcgccac 360tcgaggaatc tgagacggcg gtgggagtag ggacagttgg ttcttcagaa gccatcatgt 420tagccggatt ggccttcaaa agaaaatggc agaacaaacg caaggctgag ggtaaaccct 480atgacaaacc caacattgtc accggagcca atgttcaagt ttgctgggag aaattcgctc 540ggtacttcga ggtggagcta aaggaagtaa acctaagtga aggttactac gtgatggatc 600cagacaaagc agcagaaatg gtagacgaga acacaatctg tgtcgcagcc atattgggat 660ccacactcaa cggtgagttc gaagacgtga aacgtctcaa tgacttgcta gtcaagaaaa 720acgaggagac tggttggaac acaccgatcc acgtggatgc agcaagtgga gggttcatag 780ctccgtttat ctatcctgaa ttagaatggg actttaggct tcctttggtt aagagcatca 840acgtgagtgg tcacaagtat ggactagtct atgctggtat tggttgggtc gtgtggaggg 900cagcagagga tttacctgaa gagcttatct ttcatattaa ttatcttggt gctgatcaac 960ccactttcac tctcaatttc tccaagggat cgagccaaat tattgctcaa tactaccagc 1020tcattcgtct tggattcgag gggtacaaaa atgtgatgga gaattgcata gagaacatgg 1080tggttctcaa agaaggtata gagaaaacag agcgtttcaa catagtctca aaggaccaag 1140gagtgccagt cgtcgccttc tctctcaagg accatagttt ccacaacgag ttcgagatct 1200ctgagatgct acgtcgtttt ggctggatcg tcccagctta cactatgcct gccgatgtac 1260agcacatcac ggttctgcgt gttgtcatca gggaagattt ctcaagaaca ctcgcggaga 1320gacttgttgc tgatatttcg aaggtgcttc atgagctaga taccttgcct tccaagatat 1380ctaagaagat gggaatagaa gggatcgcgg aaaatgtaaa ggagaagaag atggagaagg 1440agattctgat ggaagttatt gttggatgga ggaagtttgt gaaggagagg aagaagatga 1500atggtgtgtg ctaagcaagt gtgttgcctt tgtgtggaaa tgaagaggta cttgcgagga 1560ctttgcgttt atcagtttat gtgtttgtat atctatttga tccagttatt atggattata 1620tacgcttgaa actcatttta agccattgtt attgaacgtt tatcaaatac tttattatgc 1680caaat 1685531771DNANicotiana tabacum 53tattttcatt ttctctcctg ttttaatttc tgatcttctc cgtcgtacta ccaccactac 60gccgccatgg ttctgtccaa gacagcgtcg gaaagtgacg tctccgttca ctccactttc 120gcctcccgat atgttcgaac ttctcttccc aggtttaaaa tgccagagaa ttcaatacca 180aaggaagcag catatcagat tataaatgat gagcttatgt tagatggaaa tccaaggcta 240aatttagcat ctttcgttac aacatggatg gagccagaat gtaatacgtt aatgatggat 300tccattaaca agaactacgt tgacatggat gaataccctg taaccactga gcttcagaat 360cgatgtgtaa atatgatagc tcatttgttt aatgcaccac ttggagatgg agagactgca 420gttggagttg gaactgttgg atcctctgaa gctattatgc ttgctggatt agcctttaag 480agaaaatggc aaaataaaat gaaagcccaa ggcaagccct ttgataagcc caatattgtc 540accggtgcta atgtccaggt gtgttgggag aaatttgcaa ggtattttga agtggagttg 600aaagaagtaa aattgagtga tggatactat gtgatggacc ctgagaaagc tgtggaaatg 660gtggatgaga ataccatttg tgttgctgct atcttaggtt caacactcaa tggtgaattt 720gaagatgtta agcgtttgaa tgaccttttg attgagaaga acaaagaaac cgggtgggac 780actccaattc atgtggatgc agcaagtggt ggatttattg caccattcct ttatccagag 840cttgaatggg actttagatt gccattggag aagagtatta atgtgagtgg tcacaaatat 900ggtcttgtct atgctggtat tggttgggcc atttggagga ataaggaaga cttgcctgat 960gaacttattt tccacatcaa ttaccttggt gctgatcaac ctactttcac tctcaacttc 1020tctaaaggtt ctagccaagt aattgctcaa tattaccaac ttattcgctt gggttttgag 1080ggttacaaga atgttatgga gaattgtcaa gaaaatgcaa gggtattaag agaaggaatt 1140gaaaaaagtg gaagattcaa cataatctcc aaagaaattg gagttccctt agtagcattt 1200tctcttaaag acaacagtca acacaatgag ttcgaaattt ctgaaactct tagaagattt 1260ggatggattg ttctggcata tactatgcca ccaaatgctc aacatgtcac agttctcaga 1320gttgtcatta gagaagattt ctcccgcaca ctagcggagc gactggtaat agacattgaa 1380aaagtcttcc acggagtaga cacacttccg gcgagggtca acgctaagct agccgtggcc 1440gaggcgaatg gcagcggcgt gcataagaaa acagatagag aagtgcagct agagattact 1500actgcatggt tgaaatttgt tgctgataag aagaagaaga ctaatggagt ttgttaattt 1560aatttaacaa aaaaaaagtt tataatatgg tgatttatgt aactactagc agtcgtactg 1620cttgtttttt atatttgagt tgatgtgttt tttgagcact tgaggagcta gctagttatt 1680gctagtgaaa aattggatga tatattttgg actactttgt aagtttgtat tattaatcca 1740aattaaacga tatttatcat aaaaaaaaaa a 1771541485DNAArabidopsis thaliana 54atggttttga caaaaaccgc aacgaatgat gaatctgtct gcaccatgtt cggatctcgc 60tatgttcgca ctacacttcc caagtatgag attggtgaga attcgatacc gaaagacgct 120gcgtatcaga tcataaaaga tgagctgatg cttgatggta acccaaggct taacctagct 180tcttttgtga ctacatggat ggaaccagag tgtgacaaac tcatcatgga ctctatcaat 240aagaactacg ttgatatgga tgagtaccct gtcacaactg agctccagaa ccgatgtgta 300aacattatag ctcgactgtt caatgcgcca ctcgaggaat ctgagacggc ggtgggagta 360gggacagttg gttcttcaga agccatcatg ttagccggat tggccttcaa aagaaaatgg 420cagaacaaac gcaaggctga gggtaaaccc tatgacaaac ccaacattgt caccggagcc 480aatgttcaag tttgctggga gaaattcgct cggtacttcg aggtggagct aaaggaagta 540aacctaagtg aaggttacta cgtgatggat ccagacaaag cagcagaaat ggtagacgag 600aacacaatct gtgtcgcagc catattggga tccacactca acggtgagtt cgaagacgtg 660aaacgtctca atgacttgct agtcaagaaa aacgaggaga ctggttggaa cacaccgatc 720cacgtggatg cagcaagtgg agggttcata gctccgttta tctatcctga attagaatgg 780gactttaggc ttcctttggt taagagcatc aacgtgagtg gtcacaagta tggactagtc 840tatgctggta ttggttgggt cgtgtggagg gcagcagagg atttacctga agagcttatc 900tttcatatta attatcttgg tgctgatcaa cccactttca ctctcaattt ctccaaggga 960tcgagccaaa ttattgctca atactaccag ctcattcgtc ttggattcga ggggtacaaa 1020aatgtgatgg agaattgcat agagaacatg gtggttctca aagaaggtat agagaaaaca 1080gagcgtttca acatagtctc aaaggaccaa ggagtgccag tcgtcgcctt ctctctcaag 1140gaccatagtt tccacaacga gttcgagatc tctgagatgc tacgtcgttt tggctggatc 1200gtcccagctt acactatgcc tgccgatgca cagcacatca cggttctgcg tgttgtcatc 1260agggaagatt tctcaagaac actcgcggag agacttgttg ctgatatttc gaaggtgctt 1320catgagctag ataccttgcc ttccaagata tctaagaaga tgggaataga agggatcgcg 1380gaaaatgtaa aggagaagaa gatggagaag gagattctga tggaagttat tgttggatgg 1440aggaagtttg tgaaggagag gaagaagatg aatggtgtgt gctaa 1485551705DNANicotiana tabacum 55aaaatatctc cattttctcc cttgttttag tctctgatct tctccgtcgt actaccacca 60ctacgccgcc atggttctgt ccaagacagc gtcggaaagt gacgtctcca tccactccac 120tttcgcttcc cgatatgttc gtacttctct tccgaggttt aagatgccag agaattcgat 180accaaaggaa gcagcatatc aaatcataaa tgatgagctt atgttagatg gaaatccaag 240actaaattta gcatcttttg tgacaacatg gatggaacca gagtgtaaca aactgatgat 300ggattccatt aacaagaatt acgttgacat ggatgaatac cctgtaacca ctgaacttca 360gaatcgatgt gtaaacatga tagctcattt gtttaacgca ccacttggag atggagagac 420tgcagttgga gttggaactg ttggatcctc tgaggctatt atgcttgctg gattagcttt 480caagagaaaa tggcaaaata aaatgaaagc ccaaggcaag ccctgtgaca agcccaatat 540tgtcactggt gccaatgtcc aggtgtgttg ggagaaattt gcaaggtatt ttgaagtgga 600gctaaaggaa gtaaagttga gtgatggata ctatgtgatg gaccctgaga aagctgtgga 660aatggtggat gagaacacaa tttgtgtagc tgctatcttg ggttccacac tcaatggtga 720atttgaagat gttaagcgct tgaatgacct cttgattgag aagaacaaag aaaccgggtg 780ggacactcca attcatgtgg atgcagcaag tggtggattt attgcaccat tcctttatcc 840agagcttgaa tgggacttta gattgccatt ggtgaagagt ataaacgtga gtggtcacaa 900atatggtctt gtttatgctg gtattggttg ggccatttgg aggaataagg aagacttacc 960tgacgaactt atcttccaca ttaattatct tggtgctgat caacctactt tcactctcaa 1020cttctctaaa ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggttt 1080tgagggttac aagaatgtta tggagaattg tcaagaaaat gcaagggtac taagagaagg 1140acttgaaaaa agtggaagat tcaacataat atccaaagaa attggagttc cattagtagc 1200tttctctctt aaagacaaca gtcaacacaa tgagttcgaa atttctgaaa ctcttagaag 1260atttggatgg attattcctg catatactat gccaccaaat gctcaacatg tcacagttct 1320cagagttgtc attagagaag atttctcccg tacactcgcc gagcgactgg taatagacat 1380tgaaaaagtc ctccacgagc tagacacact tccggcgagg gtcaacgcta agctagccgt 1440ggccgaggcg aatggcagcg gcgtgcataa gaaaacagat agagaagtgc agcttgagat 1500tactactgca tggaagaaat ttgttgctga taagaagaag aagactaacg gagtttgtta 1560atttaattta acaaaatatg tttataatta atatgatgat ttataactac tagcagtggt 1620actgcttgtt tttatatttg aattgttggg ttttttgagt atgaggagct agctatttat 1680tgctagtgaa atattggttg aaaaa 1705561929DNANicotiana tabacum 56atctccattt tctcccttgt tttagtctct gatcttctcc gtcgtactac caccactacg 60ccgccatggt tctgtccaag acagcgtcgg aaagtgacgt ctccatccac tccactttcg 120cttcccgata tgttcgtact tctcttccga ggtttaagat gccagagaat tcgataccaa 180aggaagcagc atatcaaatc ataaatgatg agcttatgtt agatggaaat ccaagactaa 240atttagcatc ttttgtgaca acatggatgg aaccagagtg taacaaactg atgatggatt 300ccattaacaa gaattacgtt gacatggatg aataccctgt aaccactgaa cttcagaatc 360gatgtgtaaa catgatagct catttgttta acgcaccact tggagatgga gagactgcag 420ttggagttgg aactgttgga tcctctgagg ctattatgct tgctggatta gctttcaaga 480gaaaatggca aaataaaatg aaagcccaag gcaagccctg tgacaagccc aatattgtca 540ctggtgccaa tgtccaggtg tgttgggaga aatttgcaag gtattttgaa gtggagctaa 600aggaagtaaa gttgagtgat ggatactatg tgatggaccc tgagaaagct gtggaaatgg 660tggatgagaa cacaatttgt gtagctgcta tcttgggttc cacactcaat ggtgaatttg 720aagatgttaa gcgcttgaat gacctcttga ttgagaagaa caaagaaacc gggtgggaca 780ctccaattca tgtggatgca gcaagtggtg aatttattgc accattcctt tatccagagc 840ttgaatggga ctttagattg ccattggtga agagtattaa cgtgagtggt cacaaatatg 900gtcttgttta tgctggtatt ggttgggcca tttggaggaa taaggaagac ttacctgacg 960aacttatctt ccacattaat tatcttggtg ctgatcaacc tactttcact ctcaacttct 1020ctaaaggttc tagccaagta attgctcaat attaccaact tattcgcttg ggttttgagg 1080gttacaagaa tgttatggag aattgtcaag aaaatgcaag ggtactaaga gaaggacttg 1140aaaaaagtgg aagattcaac ataatatcca aagaaattgg agttccatta gtagctttct 1200ctcttaaaga caacagtcaa cacaatgagt tcgaaatttc tgaaactctt agaagatttg 1260gatggattat tcctgcatat actatgccac caaatgctca acatgtcaca gttctcagag 1320ttgtcattag agaagatttc tcccgtacac tcgccgagcg actggtaata gacattgaaa 1380aagtcctcca cgagctagac acacttccgg cgagggtcaa cgctaagcta gccgtggccg 1440aggcgaatgg cagcggcgtg cataagaaaa cagatagaga agtgcagctt gagattacta 1500ctgcatggaa gaaatttgtt gctgataaga agaagaagac taacggagtt tgttaattta 1560atttaacaaa atatgtttat aattaatatg atgatttata actactagca gtggtactgc 1620ttgtttttat atttgaattg ttgggttttt tgagtatgag gagctagcta tttattgcta 1680gtgaaatatt ggttgatttt ggactacttt gtattattaa tgttaatttt cttaagtact 1740taatatgagg atatttatca tgcatgtgat atagaaaaaa gttgtgagtg cctgaggtct 1800agtttaatcc tttatattcc aatataaaaa acatatacat gggcggaggt agaaaaacca 1860atatatttgt attaagaata ttattatatt aaattttaaa ttcaattatt agatcccaaa 1920aaaaaaaaa 1929571509DNAArabidopsis thaliana 57atggtgctct cccacgccgt atcggagtcg gacgtctccg tccactccac attcgcatca 60cgttacgtcc gtacttcact tcctaggttc aagatgccgg aaaactcgat tcctaaggaa 120gcggcgtatc agatcatcaa cgacgagctg atgcttgacg ggaatccacg gttgaactta 180gcctcctttg tgacgacatg gatggagcct gagtgtgata aactcatcat gtcctccatc 240aacaagaact atgttgacat ggacgagtac cccgtcacca ccgaacttca gaaccgatgt 300gtgaacatga ttgcacatct attcaatgca ccgttagaag aggcggagac cgccgtcgga 360gtaggaaccg ttggatcatc ggaggccata atgttggccg gtttggcctt caagcgtaaa 420tggcagaaca agcgcaaagc tgaaggcaaa cccgtcgata aacccaacat tgtcaccgga 480gccaatgttc aagtgtgttg ggagaaattc gctaggtact ttgaggttga acttaaggaa 540gtgaaattga gtgaaggata ctatgtgatg gaccctcaac aagctgttga tatggttgat 600gagaacacca tttgtgttgc ggacattctt ggttccactc ttaatggaga attcgaagat 660gttaaactct tgaacgatct cttggtcgaa aagaacaaag aaaccggatg ggatacacca 720atccacgtgg atgcggcaag tggaggattc attgcaccgt ttttgtatcc ggaattggaa 780tgggacttta gacttccctt ggtgaagagt atcaatgtga gtggtcacaa gtatggactt 840gtgtacgcag ggattggttg ggtgatctgg agaaacaaag aggatttgcc tgaggaactc 900atcttccata tcaattatct tggtgctgac caacccacct ttactctcaa tttctccaaa 960ggttcaagtc aagtcattgc tcaatactac caacttatcc gattgggcca cgagggttac 1020agaaatgtga tggagaattg cagagagaat atgatcgtcc taagggaagg acttgagaag 1080acagaaaggt tcaacatcgt ctcaaaggac gagggagtgc cacttgtcgc tttctccttg

1140aaagatagca gctgtcacac tgagttcgaa atctccgaca tgcttcgcag gtatggatgg 1200atagtgccgg cctacacaat gcctccaaat gcacaacaca tcactgttct tcgtgtggtt 1260atcagagaag atttctcgag aacactcgct gagagacttg tgatcgatat agagaaagtg 1320atgcgtgagc tcgatgagct tccttcgaga gtgattcaca aaatatcact tggacaagag 1380aagagtgaat ctaacagcga taacttgatg gtcacggtga agaagagcga tatcgacaag 1440cagagagata tcatcactgg ctggaagaag tttgtcgccg acaggaagaa gacgagtggt 1500atctgctaa 1509581785DNAPetunia hybrida 58aaagagtaca aactaatatc cacttaaatt gtatttctcc attttctctc tttatttagt 60ctgtcataac aatggttcta tcaaagacag tgtcgcagag cgatgtgtcc attcactcca 120cgtttgcttc tcgatatgtt cgaacttctc ttcccaggtt taaaatgcca gataattcga 180taccaaaaga agcagcatat cagatcataa atgatgaact gatgttagat ggaaacccaa 240ggctgaactt ggcttctttt gttacaacat ggatggaacc agagtgtgat aagttgatga 300tggactctat taacaagaac tatgttgata tggatgaata tcctgttacc actgagcttc 360agaatcgatg tgtaaacatg atagctcatt tgtttaatgc accacttgaa gatggagaaa 420ctgcagttgg agttggaact gttggatcct ctgaagccat tatgcttgct ggattagctt 480tcaagagaaa atggcagaac aaaatgaaag cccaaggcaa accctgtgac aagcccaaca 540ttgttactgg tgcaaatgtc caggtgtgct gggagaaatt tgcaaggtat tttgaagtgg 600agctaaagga agtaaagctt agtgaaggat actatgtgat ggaccctgag aaagctgtgg 660agatggtgga tgaaaacacc atttgtgtag ctgctatctt aggttccacc ctcaatggag 720aatttgaaga cgttaagcgc ttgaatgatc tcttggtcga gaagaacaaa gaaaccgggt 780gggacactcc aattcatgtg gatgcagcaa gtggtggatt tattgcaccg ttcatttacc 840cagagcttga gtgggacttt agattgccat tagtgaagag cattaatgta agtggtcaca 900aatatggtct tgtctatgct ggtattggtt gggtcgtttg gaggaacaag gatgatttgc 960ctgatgaact tatcttccac attaattatc ttggtgctga tcaacctact ttcactctca 1020acttttctaa aggttctagc caagtaattg ctcaatatta ccaacttatt cgcttgggtt 1080atgagggtta caagaatgtg atggagaatt gtcaagaaaa tgcatcggta ctaagagaag 1140ggctagaaaa gacaggaaga ttcaacataa tctccaaaga aattggagta cctttagtag 1200cattctctct taaagacaac aggcaacaca acgagttcga gatttctgaa actttaagga 1260gatttggttg gattgttcct gcatatacta tgccaccaaa cgcacaacac attacagttc 1320tcagagttgt gatcagagaa gatttctccc gtacgcttgc agaacgactg gtaagagaca 1380tcgaaaaagt ccttcatgaa cttgacacac tccctgcacg tgtcaatgct aagctcgctg 1440tggccgagga gcaggcggct gcgaatggca gcgaggtgca taagaaaaca gatagcgaag 1500tgcagttgga gatgataact gcatggaaga agtttgttga agaaaagaag aagaagacta 1560atcgagtttg ttaattaatt atattagtgt ttataatatg atgaatatgg ctattatcat 1620tggtgactgc ttgttagtat attagctgtg attatcacca atatgagttt ggttttcttg 1680atttggttct tttcagtact tgaaaagttg ttattgatat tgtaaaattg tactttttaa 1740ctatttggat tattaatgcc aattttctag tgtacttaat aaaaa 178559906DNAOryza sativa 59ggagagtttg aggatgttaa gttattgaat aatctcctaa cagaaaagaa taaggaaact 60gggtgggatg tgccaattca tgttgatgca gcaagtggag gatttatagc accttttcta 120taccctgagc ttgaatggga cttcaggcta ccactggtga agagcatcaa tgtcagtggg 180cacaagtatg gccttgtgta tccaggtgtt ggttgggtca tttggcgaag caaagaggat 240ttgcctgaag aactcatttt ccatataaac tatctgggga cagaccagcc gacgttcact 300ctgaacttct ccaaaggttc cagccagata atcgcacagt actatcaact aatacgcctg 360ggattcgagg gatacaagaa catcatgcag aattgcatgg agaccccagc aatattaagg 420gaaggcatag aggcgactgg tcgattcgaa atcctctcca aggaggccgg tgtgcccttg 480gtggcgttct cgctcaaggc cagcggcagg tacaccgtgt tcgacatctc cgagcacctg 540aggaggttcg gctggatcgt gccggcgtac accatgccgg ccaacgccga gcacgtcgcc 600atcctccgcg tcgtcatcag ggaggacttc agccggagcc tcgccgagcg gctcgtctcg 660gacatcgtca agatcctgca cgagctggac gcccattcgg cccaggtgct gaagatctcc 720agcgccatcg cgaagcagca atcgggcgac gatggcgcgg tcaccaagaa gagcgtcctg 780gagaccgaga gggagatctt cgcgtactgg agggaccagg tgaagaagaa gcagaccgga 840atctgctagt gtggctctgt gagaaatgct tgaataacgt ggcatgctcg atttgtgcat 900gggatg 906601783DNALycopersicon esculentum 60aaaaaatggt gttaacaacg acgtcgataa gagattcaga agagagcttg cactgtacat 60ttgcatcaag atatgtacag gaacctttac ctaagttcaa aatgcctaaa aaatccatgc 120cgaaagaagc agcttatcag attgtaaacg acgagcttat gttggatggt aaccccaggt 180tgaatttagc ttcctttgtt agcacatgga tggagcccga gtgcgataag ctcatcatgt 240catccattaa taaaaactat gtcgacatgg atgagtatcc tgtcaccact gaacttcaaa 300atagatgtgt taacatgtta gcacatcttt tccatgcccc ggttggtgat gatgagactg 360cagttggagt tggtacagtg ggttcatcag aggcaataat gcttgctggc cttgctttca 420aacgcaaatg gcaatcgaaa agaaaagcag aaggcaaacc tttcgataag cctaatatag 480tcactggagc taatgtgcag gtctgctggg aaaaatttgc aaggtatttt gaggttgagt 540tgaaggaggt gaaactaaaa gaaggatact atgtaatgga ccctgccaaa gcagtagaga 600tagtggatga gaatacaata tgtgttgctg caatccttgg ttctactctg actggggagt 660ttgaggatgt gaagctccta aacgagctcc ttacaaaaaa gaacaaggaa accggatggg 720agacaccgat tcatgtcgat gctgcgagtg gaggatttat tgctcctttc ctctggccag 780atcttgaatg ggatttccgt ttgcctcttg tgaaaagtat aaatgtcagc ggtcacaagt 840atggccttgt atatgctggt gtcggttggg tgatatggcg gagcaaggaa gacttgcccg 900atgaactcgt ctttcatata aactaccttg ggtctgatca gcctactttt actctcaact 960tctctaaagg ttcctatcaa ataattgcac agtattatca gttaataaga cttggctttg 1020agggttataa gaacgtcatg aagaattgct tatcaaacgc aaaagtacta acagagggaa 1080tcacaaaaat ggggcggttc gatattgtct ctaaggatgt gggtgttcct gttgtagcat 1140tttctctcag ggacagcagc aaatatacgg tatttgaagt atctgagcat ctcagaagat 1200ttggatggat cgtccctgca tacacaatgc caccggatgc tgaacacatt gctgtactgc 1260gggttgtcat tagagaggat ttcagccaca gcctagctga gagacttgtt tctgacattg 1320agaaaattct gtcagagttg gacacacagc ctcctcgttt gcccaccaaa gctgtccgtg 1380tcactgctga ggaagtgcgt gatgacaagg gtgatgggct tcatcatttt cacatggata 1440ctgtagagac tcagaaagac attatcaaac attggaggaa aatcgcaggg aagaagacca 1500gcggagtctg ctaggtctgg ccacacttgt tatctgggct ccgcttccat cgccatcctg 1560tagtatgtat tacgtgtgtt gtttccatct tatgtagtag ttggtactgt aatctgtgta 1620aatgctttca tgatcttggc tctgtatatg ctaaataagc actgcatttc aagttcctgg 1680aagtatttat gtatgaatca atccgggcat aattggtaga atgccctctc tgcgtcatct 1740ttgaatttca cgtgcaataa tatttgaaat ctacacctat tat 1783611503DNAOryza sativa 61atggttctga cgcacgtcga ggcggtggag gagggcagcg aggcggcggc cgccgtgttc 60gcgtcgaggt acgtgcagga cccggtgccg aggtacgagc tcggcgagag gtcgatatcc 120aaggacgccg cgtaccagat cgtccacgac gagctcctcc tggacagcag cccgcgcctg 180aacctggcgt ccttcgtcac cacctggatg gagcccgagt gcgacaggct catcctcgag 240gccatcaaca agaactacgc cgacatggac gagtaccccg tcaccaccga gctccagaac 300cggtgcgtga acatcatagc gaggctgttc aatgcgccgg tgggcgacgg cgagaaggcg 360gtcggggtgg gcacggtggg gtcgtcggag gccataatgc tggccgggct ggcgttcaag 420cggcggtggc agaaccggcg gaaggcggcg gggaagcccc acgacaagcc caacatcgtg 480acgggggcca acgtgcaggt gtgctgggag aagttcgcgc gctacttcga ggtggagctc 540aaggaggtga agctgaccga aggctgctac gtgatggacc ccgtcaaggc cgtggacatg 600gtcgacgaga acaccatctg cgtcgccgcc atcctcggct ccaccctcac cggcgagttc 660gaggacgtca ggcgcctcaa cgacctcctc gccgccaaga acaagcggac gggttgggac 720acgccgatcc acgtcgacgc ggcgagcggc gggttcatcg cgccgttcat ctacccggag 780ctggagtggg acttccggct gccgctggtg aagagcatca acgtcagcgg ccacaagtac 840gggctcgtct acgccggcgt cgggtgggtc atctggcgca acaaggagga cctccccgag 900gagctcatct tccacatcaa ctacctcggc gccgaccagc caaccttcac gctcaacttc 960tccaaagggt ccagtcagat tattgcgcaa tattaccagt ttcttcgact cggatttgag 1020gggtacaaga gcgtgatgaa gaactgcatg gagagcgcga ggacgctccg ggagggcctg 1080gagaagacgg ggcggttcac catcatctcc aaggaggagg gcgtgccgct ggtggccttc 1140acgttcaagg acggcgccgg cgcgcaggcc ttcaggctgt cgtcgggcct gcgccggtac 1200gggtggatcg tgccggcgta cacgatgccg gcggcgctgg agcacatgac ggtgctccgc 1260gtcgtcgtcc gggaagactt cggccggccg ctcgccgagc ggttcctgtc ccacgtcagg 1320atggccctgg acgagatgga cctcgccgcc agggcccccg tgcccagggt gcagctcacc 1380atcgagctcg gccccgcccg gaccgccggc gaggaggcct cgatcagggt ggtcaagagc 1440gaggccgtgc ccgtgcgcaa gagcgtcccg ctcgtcgccg gcaaaaccaa gggcgtttgc 1500tag 1503621542DNAOryza sativa 62atggtgctct cccacggcgt gtcgggctcc gatgagtccg tccactccac gttcgcctcc 60cgctacgtcc gcacctccct ccccaggcac gcacgctcgc ctctgtctcg agcgccattg 120gcgccgatcg attcggtgat tgattgggag ttccggatgc cggagcagtc gatccccaag 180gaggcggcgt accagatcat caacgacgag ctgatgctgg acggcaaccc gcggctgaac 240ctcgcgtcgt tcgtcaccac gtggatggag cccgagtgcg acaagctcat ccaggcctcc 300gtcaacaaga actacgtcga catggacgag taccccgtca ccaccgaact ccagaaccga 360tgtgtgaaca tgattgcaca cctcttcaat gctcctctag gggactctga aacggccgtc 420ggagtcggca ctgtcggctc gtctgaggcc atcatgctcg ccggtttggc cttcaagagg 480aggtggcaga acaagatgaa ggcagccggc aagccatgcg acaagcctaa cattgtcacc 540ggcgccaatg tccaagtttg ctgggagaag ttcgcgcgat acttcgaggt tgagctcaag 600gaagtgaagc tgagtgacgg ctactacgtc atggacccag ctaaggccgt ggatatggtc 660gacgagaaca ccatctgcgt cgcggcgatc ctcgggtcga cgctgaacgg ggagttcgag 720gacgtgaagc tgctcaacga tctgctcacc aagaagaacg ctgaaacagg ctgggacacg 780ccgatccacg tggacgcggc gagcggcggg ttcatcgcgc cgttcctgta cccggagctg 840gagtgggact tccggctgcc gctggtgaag agcatcaacg tgagcgggca caagtacggc 900ctcgtctacg ccgggatcgg gtggtgcatc tggaggagca aggaggatct gcctgaggag 960ctcatcttcc acatcaacta cctcggcgcc gaccagccca ccttcaccct caacttctcc 1020aagggttcca gccaggtcat tgcacagtat taccaactaa tccgcctagg ctttgagggg 1080tacaagaaca tcatggagaa ctgccaggag aacgcgatgg tgctgaagca ggggctggag 1140aagacggggc ggttcaacat cgtgtccaag gacaacggcg tgccgctggt ggccttctcc 1200ctcaaggaca gcgcccggca caacgagttc gagatctccg acttcctccg ccgcttcggc 1260tggatcgtgc cggcctacac catgcccccc gacgcgcagc acgtcaccgt gctccgcgtc 1320gtcatccgcg aggacttcag ccgcacgctc gccgagcgcc tcgtgctcga cgtcgagaag 1380gtgctgcacg agctcgacgc gctccccgcc cgcgtcgtcg ccaacggcgg cgacgccgcc 1440gccgcgtcgg cgagcgagag ggagatggag aagcagcgcg aggtgatctc cctctggaag 1500agggccgtgc tggccaagaa gaagaccaac ggcgtctgct aa 1542633983DNAOryza sativa 63ctcgatcatt aactgtgagt taagtgagtt atttgtacac ttattaacta gttccgcgag 60catatcttaa atacatgaca tgtcatgtag aattgaaaag ttacatataa aattaaaaaa 120aacatgtatt tttttccttt cccatggtac tacaattggc aatgcaaaaa attagtaaaa 180ccaaatcatt ttagtttcat gttttgaaga gtgtagttct cggcttgcca tcaaacatga 240gcacctttta ttttcgatct ccatgttctt ttccacccaa ttacatctgc acatttttag 300aagtattttt tttcaatcct agatgacatg ccacgtgtac aaggtatgct cacttggata 360tgtagtttga tggacgtaca tatgacttac ttaactcaga ctagtgatcc aattgtgtat 420tttgaaagta gagtgcacat atatgataca tataaacaag ttgaatggca cttcatgcat 480tttactctta cgtctatcat aaaaaaacaa aaagaagcaa tcctcaactc atatacccag 540gtaaaaacac acctttgcaa acttaaacgg tgtgagccaa tatccatact atcaagattt 600gaaatgtatc caattaggcc aacctacacc aaaacttttt ttttaataat ggaacagaat 660cccaccaaaa actaaatatg ctcaatgttt ctatttgtga agttatctgg gggagagcag 720tttataattt gagtgcatat gctttaatat tttatatgtc attcaaatgg ttgttttttt 780aaaagaaaat atagtgccac gttatatatc agtccatcaa cacacaaatc taaataaact 840tgtactaagt tgtaaaaatc caactagagt actatccaac ctacatgggg atgtgatacg 900tatcatctag cgcaacaaat ctaaagaggg aattatctaa atttattgca tgtccatatt 960cactgtccta aatggtgtta ctcccatcta gatcgggttt gtttggaatg gaaggagtat 1020gtccagatac atacaaaatg aatgtaccaa aaaagtcaaa gtgatttata atgtagaacg 1080gagggagtat catactacaa tccttttgaa aacgatatca tttctttatt atccttttgg 1140aaactatatc ggtaccgtcg aggatttttc ataattttga aatttagcca cttcgatagg 1200atgcaaatat aaattgaaaa aaactaaatt ccataataaa aaacaatata attttctatt 1260atttaacggt atcggtactg tcgaaaattt ttcatactgt tttcatccct attcaggatg 1320aatgagacgc aacaacacga gacgtcgaga cccctcacgg aggcacgaaa aatccatttc 1380ttgatgcggg ggaaggagtg ttagctcaac cgggggggct aatcactatc gaataattta 1440acaagccacg caacgaagca gcaggaggaa gcaaccaaag gctagcaagc aaagcagcac 1500ctcagcatta cgcaataatt caggccgaag cagagtcacc cagcagcaag aacatgggac 1560aagcaaagca caagccacat tctaaggtag tgtacaagct agctgacctg ggagtccagt 1620atataagtgc gtgtagccgc atgtacacag ggtggccaaa acgagacgtg cagagagaga 1680caccaatctc gtacgtagca tatctacatc gcaggaacac ccaaatcact cgtgcgtagg 1740tatggttctg acgcacgtcg aggcggtgga ggagggcagc gaggcggcgg ccgccgtgtt 1800cgcgtcgagg tacgtgcagg acccggtgcc gaggtacgag ctcggcgaga ggtcgatatc 1860caaggacgcc gcgtaccaga tcgtccacga cgagctcctc ctggacagca gcccgcgcct 1920gaacctggcg tccttcgtca ccacctggat ggagcccgag tgcgacaggc tcatcctcga 1980ggccatcaac aagaactacg ccgacatgga cgagtacccc gtcaccaccg agctccaggc 2040aagcaatcaa acaaaccaaa ccatataccc tcgtctcctc catatccttt cgttgcattg 2100cgttgtggtt gacgtacgac gacgatggcc ctggcgttgc agaaccggtg cgtgaacatc 2160atagcgaggc tgttcaatgc gccggtgggc gacggcgaga aggcggtcgg ggtgggcacg 2220gtggggtcgt cggaggccat aatgctggcc gggctggcgt tcaagcggcg gtggcagaac 2280cggcggaagg cggcggggaa gccccacgac aagcccaaca tcgtgacggg ggccaacgtg 2340caggtgtgct gggagaagtt cgcgcgctac ttcgaggtgg agctcaagga ggtgaagctg 2400accgaaggct gctacgtgat ggaccccgtc aaggccgtgg acatggtcga cgagaacacc 2460atctgcgtcg ccgccatcct cggctccacc ctcaccggcg agttcgagga cgtcaggcgc 2520ctcaacgacc tcctcgccgc caagaacaag cggacgggtt gggacacgcc gatccacgtc 2580gacgcggcga gcggcgggtt catcgcgccg ttcatctacc cggagctgga gtgggacttc 2640cggctgccgc tggtgaagag catcaacgtc agcggccaca agtacgggct cgtctacgcc 2700ggcgtcgggt gggtcatctg gcgcaacaag gaggacctcc ccgaggagct catcttccac 2760atcaactacc tcggcgccga ccagccaacc ttcacgctca acttctccaa aggcacgaaa 2820ttatttttcc agaattaaat gatccgtaca tatatagagt ggagtagtac attttgtgac 2880ttggcaagtt catttttgtg cagggtccag tcagattatt gcgcaatatt accagtttct 2940tcgactcgga tttgaggtat actgagacta attgccccaa aatttcgaat ttaaaatttt 3000gaaaatttta tgtcaaactc ctataatttc gatttttttt ttggccggat gcacatccta 3060ccggaacaca ccggcataac tgaaatttcg caaaattctt tctgaaattg tgcacattta 3120tttcagtttg attgaaccgt tgggttgttt gattgttgca ggggtacaag agcgtgatga 3180agaactgcat ggagagcgcg aggacgctcc gggagggcct ggagaagacg gggcggttca 3240ccatcatctc caaggaggag ggcgtgccgc tggtggcctt cacgttcaag gacggcgccg 3300gcgcgcaggc cttcaggctg tcgtcgggcc tgcgccggta cgggtggatc gtgccggcgt 3360acacgatgcc ggcggcgctg gagcacatga cggtcgtccg cgtcgtcgtc cgggaagact 3420tcggccggcc gctcgccgag cggttcctgt cccacgtcag gatggccctg gacgagatgg 3480acctcgccgc cagggccccc gtgcccaggg tgcagctcac catcgagctc ggccccgccc 3540ggaccgccgg cgaggaggcc tcgatcaggg tggtcaagag cgaggccgtg cccgtgcgca 3600agagcgtccc gctcgtcgcc ggcaaaacca agggcgtttg ctagaccggg ttaaattttt 3660ttttaaaata ctggtgggaa cacgaccaaa ataaaatttc aaaatgggag cgtactaaac 3720cttctttatg ctacagtaac tatgtagtac aagcatgccg aaagttaatc atcgtgtgta 3780atcgtattag agtttgcaac agcatattat aatgtggaga agaacacgca agcgacatca 3840accaggactc tgcacttctg atgaagttct gaaacccata aggattcaga taaacatgag 3900cagacactga aatatgtatg tacataaaaa atttgtgcga ggcatgcaca tcatgcttga 3960gcacgttatt gttgcagaaa aca 3983642064DNAOryza sativa 64aaggagagag ccttcccgtc tccttttttt ccttccatcc aaaaaattcc aaatggagta 60gtagttcaca tttttacacc gtcgtcttcg caaatttaat actactatac gaaggaggag 120gaagctctcg cgccgcggtc gctttccttc ccctaaatta aatcccgtgc gacgcgtaga 180gacctcctcc tccgctttct cctccaaagc ccaagctaat ccccctcctc ttcctcgtcg 240ccggcgagag ctcttcgtct tctccaactc gaccggaagc aagcgcgctt ccgatccgcc 300atggtgctct cccacggcgt gtcgggctcc gatgagtccg tccactccac gttcgcctcc 360cgctacgtcc gcacctccct ccccaggttc cggatgccgg agcagtcgat ccccaaggag 420gcggcgtacc agatcatcaa cgacgagctg atgctggacg gcaacccgcg gctgaacctc 480gcgtcgttcg tcaccacgtg gatggagccc gagtgcgaca agctcatcca ggcctccgtc 540aacaagaact acgtcgacat ggacgagtac cccgtcacca ccgaactcca gaaccgatgt 600gtgaacatga ttgcacacct cttcaatgct cctctagggg actctgaaac ggccgtcgga 660gtcggcactg tcggctcgtc tgaggccatc atgctcgccg gtttggcctt caagaggagg 720tggcagaaca agatgaaggc agccggcaag ccatgcgaca agcctaacat tgtcaccggc 780gccaatgtcc aagtttgctg ggagaagttc gcgcgatact tcgaggttga gctcaaggaa 840gtgaagctga gtgacggcta ctacgtcatg gacccagcta aggccgtgga tatggtcgac 900gagaacacca tctgcgtcgc ggcgatcctc gggtcgacgc tgaacgggga gttcgaggac 960gtgaagctgc tcaacgatct gctcaccaag aagaacgctg aaacaggctg ggacacgccg 1020atccacgtgg acgcggcgag cggcgggttc atcgcgccgt tcctgtaccc ggagctggag 1080tgggacttcc ggctgccgct ggtgaagagc atcaacgtga gcgggcacaa gtacggcctc 1140gtctacgccg ggatcgggtg gtgcatctgg aggagcaagg aggatctgcc tgaggagctc 1200atcttccaca tcaactacct cggcgccgac cagcccacct tcaccctcaa cttctccaag 1260ggttccagcc aggtcattgc acagtattac caactaatcc gcctaggctt tgaggggtac 1320aagaacatca tggagaactg ccaggagaac gcgatggtgc tgaagcaggg gctggagaag 1380acggggcggt tcaacatcgt gtccaaggac aacggcgtgc cgctggtggc cttctccctc 1440aaggacagcg cccggcacaa cgagttcgag atctccgact tcctccgccg cttcggctgg 1500atcgtgccgg cctacaccat gccccccgac gcgcagcacg tcaccgtgct ccgcgtcgtc 1560atccgcgagg acttcagccg cacgctcgcc gagcgcctcg tgctcgacgt cgagaaggtg 1620ctgcacgagc tcgacgcgct ccccgcccgc gtcgtcgcca acggcggcga cgccgccgcc 1680gcgtcggcga gcgagaggga gatggagaag cagcgcgagg tgatctccct ctggaagagg 1740gccgtgctgg ccaagaagaa gaccaacggc gtctgctaaa gcaagtcagc tgcgcagcgg 1800ggatcgtata ctattattat ggtccacttc gtaattgcga cttcttaatt tttaccgtgt 1860cgatcggttt accgtctcaa gtctgctctc atccgatttt cggattgatg ctgcttaatt 1920atgtaccaag ttaattaatt cttagaggac ggaatagttc cgactcaata cgaagcattg 1980cctgtataag tcgaacacgg gatcatgcat tttacgttgg gtgtgtagca gtgaattgat 2040cagtgatgct ccatctctac tctg 2064651473DNAHordeum vulgare 65atggtggtga ccgtggcagc gacggggccg gacacggccg agacgctgca ctccaccacc 60ttcgcctccc gctacgtccg cgaccagctc ccccggtacc ggatgccgga gaactcgatc 120cccaaggagg cggcgtacca gatcatcagc gacgagctga tgctggacgg caacccgcgg 180ctgaacctgg cgtccttcgt caccacctgg atggagcccg agtgcggcaa gctcatcatg 240gactccgtca acaagaacta cgtcgacatg gacgagtacc ccgtcaccac cgagctccag 300gaccgttgcg taaacatgat agctcacttg ttcaatgcac cgatcggcga ggacgagaca 360gctatcggag tctcgacggt ggggtcttcg gaagcaatca tgcttgcagg cctggcgttc 420aagaggaagt gggcgaacaa aatgaaggag caggggaagc catgcgacaa acctaacatt 480gttactggtg caaatgttca ggtttgctgg gagaaatttg ctaggtattt tgaagtggaa 540ttgaaggagg tcaagttgac tgaagggtac tatgtcatgg atcctaagaa ggctgttgaa 600atggtggatg agaacactat atgtgtcgcc

gccatcctgg gatctactct cactggagag 660tacgaagatg tcaaactgtt gaatgacctt cttgtggaga agaacaagga aacagggtgg 720aacgtgccga tccatgttga tgctgccagc ggaggattta tcgctccgtt tcttcagcct 780gagcttgaat gggacttcag gctaccattg gtgaagagca tcaacgttag tgggcacaag 840tatggccttg tgtaccctgg tgttggatgg gtcatctggc ggagcaagga cgatttgccc 900gaagaactca ttttccacat aaactatcta ggagcagatc agcccacatt cacgctcaac 960ttctccaagg gtcagcagat catcgcgcaa tactatcagc tcatccgcct cggcttcgag 1020gggtacaagc acatcatgga gaactgcaag ctgaacgcgg cggtgctgaa ggagggcatc 1080gacgcgacgg ggcggttcga cgtgctgtcc aaggcggacg gcgtgccgct ggtggccatc 1140cggctcaagg acagcaccaa cttcagcgtg ttcgacatct cggagaacct gaggcggttc 1200gggtggatcg tgccggcgta caccatgccc gccgacgcgg agcatgtggc cgtgctccgc 1260atagtcatcc gggaggactt caaccggagc ctcgcgcagc ggctcctcgc cgacatcaac 1320aagatcatcg gcgagctgga cgcgcacgcc gtccacgcca tcaagctctc caccgccgcc 1380gctggtgggg acggcgcgag taagagcgcg gtcgacgccg ccaccgaggc cttcaaggac 1440ctggcgggga agaagaaggc cggagtatgc tga 14736611688DNAArtificialvector 66ggaattcgat atcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc 60ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 120gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct 180agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt 240ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat 300atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca 360gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg 420cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac 480gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat 540aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc 600tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc 660cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg 720cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt 780gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat 840ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc 900ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat 960catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc 1020ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg 1080ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc 1140acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga 1200ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca 1260agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag 1320gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg 1380ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg 1440gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc 1500ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg 1560aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg 1620caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg 1680atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg 1740tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg 1800tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg 1860acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg 1920tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg 1980tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt 2040acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg 2100ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg 2160cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag 2220caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac 2280gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga 2340ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct 2400atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa 2460ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg 2520aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc 2580ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgacgg tcgcaaacca 2640tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc 2700gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa 2760gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg 2820attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac 2880gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt 2940gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc 3000gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc 3060catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg 3120ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag 3180aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt 3240acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc 3300cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct 3360gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc 3420gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc 3480gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc 3540ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg 3600gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc 3660aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa 3720attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt 3780gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac 3840attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc 3900gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg 3960tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc 4020ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc 4080ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg 4140cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 4200catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 4260ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 4320tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 4380gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg 4440cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 4500gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 4560aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 4620gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 4680aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 4740gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 4800ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 4860cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 4920ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 4980actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 5040tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 5100gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 5160ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 5220cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 5280ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc 5340tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg 5400atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg 5460cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct 5520cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca 5580tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg 5640gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta 5700tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg 5760ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc 5820tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca 5880ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc 5940cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta 6000tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt 6060tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac 6120agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc 6180ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg 6240cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc 6300tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg 6360cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac 6420aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt 6480tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt 6540aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat 6600taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta 6660gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg 6720ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca 6780cattattatg gagaaactcg agcttgtcga tcgacagatc cggtcggcat ctactctatt 6840tctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac 6900agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg 6960ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc 7020cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagtc 7080gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac 7140aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca 7200tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg 7260agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca 7320gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt 7380gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga 7440ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg 7500catccatagc ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt 7560gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctaaactccc 7620caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac 7680gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta 7740catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc 7800tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca 7860tatctcattg cccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac 7920tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaaggtctt 7980gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca 8040cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg 8100gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc 8160aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga 8220tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa 8280tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt 8340gctccaccat gttatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt 8400tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat 8460cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt 8520ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg 8580atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga 8640tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc tctagccaat 8700acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 8760tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 8820ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 8880ataacaattt cacacaggaa acagctatga ccatgattac gaattccctt aattaaggcg 8940cgccgatact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt 9000ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa 9060gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct 9120gggaactact cacacattat tatggagaaa ccaggccgga gccaggtatt cacggttcag 9180atatactcca ggaaaaatag atacatatag tacgtacgtg cgtactgtgc cggcatgccc 9240ttcagcaggg tccagtcttc ttgttcttta ccaggtcctt gcaggccgcg gcgaactcct 9300tctcgatgtc cagaacgccc ttcttggtca ccacgccatc ctcagcaccc tcgccggttt 9360gtgtcgcgat agcagtggtc atcttaatgg catggaccgc gtgggcatct agctcctgca 9420cgaccctgtt gatgtcggcg aggagccgct gggagaggct gcggttgaag tcctccctga 9480tgacgacgcg gaggacagcc acgtgctctg cgtccgcagg catggtgtag gcaggcacaa 9540tccagccaaa cctcctcagg ttctcggaga tgtcgaacac gctgaatttg gagctgtcct 9600tgagccggat ggccactagg ggcacaccgt cctctttgga caggatgtcg aatcgcccgg 9660ttgcctctaa gccctccctc agcgcggttg cattggcctg gcaattctcc atgatgtgct 9720tatatccctc gaagcctagg cgtatcagtt gatagtattg cgcaatgatc tggctagcac 9780ctttggagaa gttcaatgtg aatgtgggct gatctgttcc caggtagttt atatggaaaa 9840tgagttctcc aggcaaatcg tctttgctcc gccagatgac ccatccaaca ccagggtaga 9900cgaggccata cttgtgccca ctaacattga tgctcttcac caatggtagc ctgaagtccc 9960actcaagctc agggtgaaga aaaggggcta taaatcctcc acttgcagca tcaacatgga 10020tcggcacatt aaaccctgtt ttcttgttct tttccacaag caggtcattc aatagtttaa 10080catcttcata ctctccagtg agagtagatc ccaagatggc tgcaacacat atagtgttct 10140catccaccat ttcaacagct ttcaaaggat ccatgacata gtatccttca gttaacttga 10200cctccttcaa ttctacttca aaatatctag caaatttttc ccagcaaacc tgaacatttg 10260caccagtaac aatgttaggt ttgtcatatg gcttcccctc ctcctttctt ttatttgccc 10320acttcctctt gaaggccagg cctgcaagca ttattgcttc tgaggatccc actgtcgcaa 10380ctccaattgc tgtctcctcc tccttgatcg gtgcattgaa caggtgggct atcatattta 10440cacaacggtt ctggagctcg gtggtgacgg ggtactcgtc catgtcgacg tagttcttgt 10500tgacggagtc catgatgagc ttgccgacct cgggctccat ccgggtggtg acgaaggatg 10560cgaggttgag gcgcgggttg ccgtcgagca tgagctcgtc gctgatgatc tggtacgccg 10620cctccttggg gatcgagttc tccggcatcc ggtaccgggg gagctggtcg cggacgtagc 10680gggtggcgaa gaaggtggag ttgagcggct cggccgtgtc cgcggcggtc gctgccacag 10740tgagaaccat ggttgctggc ggtcgcggtc gcggcttgca cggagacagt gacggcaggt 10800cggccgcgcg cagcgaaggg aatcggccgc gagctcgagg aaaggtggtg ccgtggagat 10860gctaatggag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa 10920atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc 10980atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac 11040gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc 11100agagcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct 11160tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg 11220tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggccttc tgagactgta 11280tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac atcaatccac 11340ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg 11400tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca 11460atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat 11520agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat 11580agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg 11640ctccaccatg ttggcaagct gctcttatta attaaggcgc gccctgca 11688671509DNAArabidopsis thaliana 67atggtgctct cccacgccgt atcggagtcg gacgtctccg tccactccac attcgcatca 60cgttacgtcc gtacttcact tcctaggttc aagatgccgg aaaactcgat tcctaaggaa 120gcggcgtatc agatcatcaa cgacgagctg atgcttgacg ggaatccacg gttgaactta 180gcctcctttg tgacgacatg gatggagcct gagtgtgata aactcatcat gtcctccatc 240aacaagaact atgttgacat ggacgagtac cccgtcacca ccgaacttca gaaccgatgt 300gtgaacatga ttgcacatct attcaatgca ccgttagaag aggcggagac cgccgtcgga 360gtaggaaccg ttggatcatc ggaggccata atgttggccg gtttggcctt caagcgtaaa 420tggcagaaca agcgcaaagc tgaaggcaaa cccgtcgata aacccaacat tgtcaccgga 480gccaatgttc aagtgtgttg ggagaaattc gctaggtact ttgaggttga acttaaggaa 540gtgaaattga gtgaaggata ctatgtgatg gaccctcaac aagctgttga tatggttgat 600gagaacacca tttgtgttgc ggacattctt ggttccactc ttaatggaga attcgaagat 660gttaaactct tgaacgatct cttggtcgaa aagaacaaag aaaccggatg ggatacacca 720atccacgtgg atgcggcaag tggaggattc attgcaccgt ttttgtatcc ggaattggaa 780tgggacttta gacttccctt ggtgaagagt atcaatgtga gtggtcacaa gtatggactt 840gtgtacgcag ggattggttg ggtgatctgg agaaacaaag aggatttgcc tgaggaactc 900atcttccata tcaattatct tggtgctgac caacccacct ttactctcaa tttctccaaa 960ggttcaagtc aagtcattgc tcaatactac caacttatcc gattgggcca cgagggttac 1020agaaatgtga tggagaattg cagagagaat atgatcgtcc taagggaagg acttgagaag 1080acagaaaggt tcaacatcgt ctcaaaggac gagggagtgc cacttgtcgc tttctccttg 1140aaagatagca gctgtcacac tgagttcgaa atctccgaca tgcttcgcag gtatggatgg 1200atagtgccgg cctacacaat gcctccaaat gcacaacaca tcactgttct tcgtgtggtt 1260atcagagaag atttctcgag aacactcgct gagagacttg tgatcgatat agagaaagtg 1320atgcgtgagc tcgatgagct tccttcgaga gtgattcaca aaatatcact tggacaagag 1380aagagtgaat ctaacagcga taacttgatg gtcacggtga agaagagcga tatcgacaag 1440cagagagata tcatcactgg ctggaagaag tttgtcgccg acaggaagaa gacgagtggt 1500atctgctaa 1509681485DNAArabidopsis thaliana 68atggttttga caaaaaccgc aacgaatgat gaatctgtct gcaccatgtt cggatctcgc 60tatgttcgca ctacacttcc caagtatgag attggtgaga attcgatacc gaaagacgct 120gcatatcaga tcataaaaga tgagctgatg cttgatggta acccgaggct taacctagct 180tcgtttgtga ctacatggat ggaaccagag tgtgacaaac tcatcatgga ctctatcaac 240aagaactacg ttgatatgga tgagtaccct gtcacaactg agctccagaa ccgatgtgta 300aacattatag ctcgactgtt caatgcgcca ctcgaggaat ctgagacggc ggtgggagta 360gggacagttg gttcttcaga agccatcatg ttagccggat tggccttcaa aagaaaatgg 420cagaacaaac gcaaggctga gggtaaaccc tatgacaaac ccaacattgt cactggagcc 480aatgttcaag tttgctggga gaaattcgct cggtacttcg aggtggagct aaaggaagta 540aacctaagtg aaggttacta cgtgatggat ccagacaaag cagcagaaat ggtagacgag 600aacacaatct gtgtcgcagc catattggga tccacactca acggtgagtt cgaagacgtg 660aaacgtctca atgacttgct agtcaagaaa aacgaggaga ctggttggaa cacaccgatc 720cacgtggatg cagcaagtgg agggttcata gctccgttta tctatcctga attagaatgg 780gactttagac ttcctttggt taagagtatc aacgtgagtg gtcacaagta tggactggtc

840tatgctggta ttggttgggt cgtgtggagg gcagcagagg atttgcctga agagcttatc 900tttcatatta attatcttgg tgctgatcaa cccactttca ctctcaattt ctccaaggga 960tcgagccaaa ttattgctca atactaccag ctcattcgtc ttggattcga ggggtacaaa 1020aatgtgatgg agaattgcat agagaacatg gtggttctca aagaagggat agagaaaaca 1080gagcgtttca acatagtctc aaaggaccaa ggagtgccag tcgtagcctt ctctctcaag 1140gaccatagtt tccacaacga gttcgagatc tctgagatgc tacgtcgttt tggctggatc 1200gtcccagctt acactatgcc tgccgatgca cagcacatca cggttctgcg tgttgtcatc 1260agggaagatt tctcaagaac actcgcggag agacttgttg ctgatatttc gaaggtgctt 1320catgagctag ataccttgcc ttccaagata tctaagaaga tgggaataga agggatcgcg 1380gaaaatgtaa aggagaagaa gatggagaag gagattctga tggaagttat tgttggatgg 1440aggaagtttg tgaaggagag gaagaagatg aatggtgtgt gctaa 1485691503DNAPetunia x hybrid 69atggttctat caaagacagt gtcgcagagc gatgtgtcca ttcactccac gtttgcttct 60cgatatgttc gaacttctct tcccaggttt aaaatgccag ataattcgat accaaaagaa 120gcagcatatc agatcataaa tgatgaactg atgttagatg gaaacccaag gctgaacttg 180gcttcttttg ttacaacatg gatggaacca gagtgtgata agttgatgat ggactctatt 240aacaagaact atgttgatat ggatgaatat cctgttacca ctgagcttca gaatcgatgt 300gtaaacatga tagctcattt gtttaatgca ccacttgaag atggagaaac tgcagttgga 360gttggaactg ttggatcctc tgaagccatt atgcttgctg gattagcttt caagagaaaa 420tggcagaaca aaatgaaagc ccaaggcaaa ccctgtgaca agcccaacat tgttactggt 480gcaaatgtcc aggtgtgctg ggagaaattt gcaaggtatt ttgaagtgga gctaaaggaa 540gtaaagctta gtgaaggata ctatgtgatg gaccctgaga aagctgtgga gatggtggat 600gaaaacacca tttgtgtagc tgctatctta ggttccaccc tcaatggaga atttgaagac 660gttaagcgct tgaatgatct cttggtcgag aagaacaaag aaaccgggtg ggacactcca 720attcatgtgg atgcagcaag tggtggattt attgcaccgt tcatttaccc agagcttgag 780tgggacttta gattgccatt agtgaagagc attaatgtaa gtggtcacaa atatggtctt 840gtctatgctg gtattggttg ggtcgtttgg aggaacaagg atgatttgcc tgatgaactt 900atcttccaca ttaattatct tggtgctgat caacctactt tcactctcaa cttttctaaa 960ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggtta tgagggttac 1020aagaatgtga tggagaattg tcaagaaaat gcatcggtac taagagaagg gctagaaaag 1080acaggaagat tcaacataat ctccaaagaa attggagtac ctttagtagc attctctctt 1140aaagacaaca ggcaacacaa cgagttcgag atttctgaaa ctttaaggag atttggttgg 1200attgttcctg catatactat gccaccaaac gcacaacaca ttacagttct cagagttgtg 1260atcagagaag atttctcccg tacgcttgca gaacgactgg taagagacat cgaaaaagtc 1320cttcatgaac ttgacacact ccctgcacgt gtcaatgcta agctcgctgt ggccgaggag 1380caggcggctg cgaatggcag cgaggtgcat aagaaaacag atagcgaagt gcagttggag 1440atgataactg catggaagaa gtttgttgaa gaaaagaaga agaagactaa tcgagtttgt 1500taa 1503701509DNASolanum lyopersicum 70atggtgttaa caacgacgtc gataagagat tcagaagaga gcttgcactg tacatttgca 60tcaagatatg tacaggaacc tttacctaag ttcaaaatgc ctaaaaaatc catgccgaaa 120gaagcagctt atcagattgt aaacgacgag cttatgttgg atggtaaccc caggttgaat 180ttagcttcct ttgttagcac atggatggag cccgagtgcg ataagctcat catgtcatcc 240attaataaaa actatgtcga catggatgag tatcctgtca ccactgaact tcaaaataga 300tgtgttaaca tgttagcaca tcttttccat gccccggttg gtgatgatga gactgcagtt 360ggagttggta cagtgggttc atcagaggca ataatgcttg ctggccttgc tttcaaacgc 420aaatggcaat cgaaaagaaa agcagaaggc aaacctttcg ataagcctaa tatagtcact 480ggagctaatg tgcaggtctg ctgggaaaaa tttgcaaggt attttgaggt tgagttgaag 540gaggtgaaac taaaagaagg atactatgta atggaccctg ccaaagcagt agagatagtg 600gatgagaata caatatgtgt tgctgcaatc cttggttcta ctctgactgg ggagtttgag 660gatgtgaagc tcctaaacga gctccttaca aaaaagaaca aggaaaccgg atgggagaca 720ccgattcatg tcgatgctgc gagtggagga tttattgctc ctttcctctg gccagatctt 780gaatgggatt tccgtttgcc tcttgtgaaa agtataaatg tcagcggtca caagtatggc 840cttgtatatg ctggtgtcgg ttgggtgata tggcggagca aggaagactt gcccgatgaa 900ctcgtctttc atataaacta ccttgggtct gatcagccta cttttactct caacttctct 960aaaggttcct atcaaataat tgcacagtat tatcagttaa taagacttgg ctttgagggt 1020tataagaacg tcatgaagaa ttgcttatca aacgcaaaag tactaacaga gggaatcaca 1080aaaatggggc ggttcgatat tgtctctaag gatgtgggtg ttcctgttgt agcattttct 1140ctcagggaca gcagcaaata tacggtattt gaagtatctg agcatctcag aagatttgga 1200tggatcgtcc ctgcatacac aatgccaccg gatgctgaac acattgctgt actgcgggtt 1260gtcattagag aggatttcag ccacagccta gctgagagac ttgtttctga cattgagaaa 1320attctgtcag agttggacac acagcctcct cgtttgccca ccaaagctgt ccgtgtcact 1380gctgaggaag tgcgtgatga caagggtgat gggcttcatc attttcacat ggatactgta 1440gagactcaga aagacattat caaacattgg aggaaaatcg cagggaagaa gaccagcgga 1500gtctgctag 1509711500DNAPopulus tremula x Populus alba 71atggttctct ccaagacagc ttcagaatct gatgtctccg ttcactcgac ttttgcctcc 60agatatgttc gagcttcact tcccaggttc aagatgccgg agaactcgat cccgaaggag 120gcggcctttc agatcataaa cgatgagctc atgctggatg gaaaccctag gctgaacctc 180gcatcgtttg tgacgacatg gatggagcct gaatgtgaca agcttatcat cgcttctatc 240aacaaaaact acgtcgacat ggatgaatac ccggtcacca ccgagcttca gaatcgatgc 300gttaacatga tagcccatct cttcaatgct ccacttggag actcggagac agcggttgga 360gtaggaactg ttggatcttc tgaggctata atgttggctg gtctggcctt caagaggaag 420tggcaaaaca agagaaaagc tgagggaaag ccttacgata aacctaacat tgtcactgga 480gccaatgttc aggtatgctg ggagaaattt gcaaggtact ttgaggtgga gttgaaggag 540gtgaagctta gtgatggcta ttatgtgatg gatcctgaga aagcagtgca aatggtggat 600gagaacacaa tctgtgttgc agctattctt ggttccactc ttaatggaga atttgaagat 660gtcaagctct tgaatgatct cctggtggag aagaacaaat caacaggttg ggatactccg 720atccatgtcg atgcggccag cggcggcttc attgcacctt ttatataccc ggagctcgag 780tgggatttcc ggttgccatt ggtgaagagt atcaatgtca gtgggcacaa atatggactc 840gtctatgctg gtattggatg ggtcatttgg aggaacaagg aggacttgcc tgaagaactc 900atcttccata tcaattatct tggagctgat caaccaacct tcacccttaa cttctccaaa 960ggatctagtc aagttattgc tcaatattac caactcattc ggttgggtta cgagggatac 1020aaaaatgtta tggaaaattg tagagacaac atgctggtgc tgaaacaagg tctagagaag 1080acaggcaagt tcaacattgt ttcgaaagac aagggggtgc cactggtggc cttttctttg 1140aaggacaata gcctccacaa cgaatttgag gtgtccgaca tgttaaggcg ttttggttgg 1200attgtgcctg cctacaccat gcctcccgat gctcaacatg ttactgtgct gcgcgtcgta 1260atccgagaag atttttctcg gacactcgct gagcgtcttg tcattgacat tggcaaggtt 1320cttcatgagc ttgagacgtt gccgtccagg atcagtgcca agattgtgtt ggctaatgaa 1380gagaaggatg ccgtggccgc cggtaaagag aagaaggacg tccagaacga aacaagagag 1440attattacag cttggaggaa gcttgtcgtg caaaggaaga agttgaatgg tgtttgctag 1500721479DNAOryza sativa Indica 72atggtgctct ccaaggccgt ctccgagagt gacatgtccg tgcactccac cttcgcctcc 60cgctacgtcc gcgcctccct cccaaggtac cggatgccgg agaactcgat cccgaaggag 120gcggcgtacc agatcatcaa cgacgagctg atgctggacg gcaacccgcg gctgaacctg 180gcgtcgttcg tcaccacgtg gatggagccc gagtgcgaca agctcatcat ggccgccatc 240aacaagaact acgtcgacat ggacgagtac cccgtcacca ccgagctcca gaaccggtgc 300gtgaacatga tcgcgcacct gttccacgcg ccgctcgggg aggacgagac ggcggtgggc 360gtgggcacgg tgggttcgtc ggaggccatc atgctggccg ggctggcctt caagcggcgg 420tggcagaaca agcgcaaggc cgaggggaag ccgttcgaca agcccaacat catcaccggc 480gccaacgtgc aggtgtgctg ggagaagttc gcccgctact tcgaggtgga gctcaaggag 540gtgaagctcc gcgacggcta ctacgtcatg gaccccgaga aggccgtcga catggtcgac 600gagaacacca tctgcgtcgc cgccatcctc ggctccaccc tcaacggcga gttcgaggac 660gtcaagctac tcaacgacct cctcgacaag aagaacaagg agactgggtg ggagacgccg 720atccacgtgg acgcggcgag cggcgggttc atcgcgccgt tcctgtaccc ggagctggag 780tgggacttcc ggctgccgtg ggtgaagagc atcaacgtga gcggtcacaa gtacgggctc 840gtctacgccg gcatcggctg gtgcatctgg cgcaacaagg aggacctgcc cgaggagctc 900atcttccaca tcaactacct cggcgccgac cagccaacct tcaccctcaa cttctccaag 960ggctccagcc aggtcatcgc ccagtactac cagctcatcc gccacggctt cgaggggtac 1020aggaacatca tggagaactg ccacgagaac gcgatggtgc tgaaggaagg gctggtgaag 1080acggggaggt tcgacatcgt gtccaaggac gaaggcgtgc cgctggtggc gttctcgctc 1140aaggaccgga gccggcacga cgagttcgag atctccgaca tgctgcgccg cttcggctgg 1200atcgtgccgg cgtacaccat gccgcccgac gcccagcacg tcacggtgct ccgcgtggtc 1260atccgggagg agttcagccg caccctcgcc gagcgcctcg tcctcgacat cgagaaggtg 1320atgtaccagc tcgacgcgct cccctccagg ctcatgcccc ccgtgccgcc ggcgccgctg 1380cttgtggtcg ccaagaagtc ggagctcgag acgcagcggt cggtgacgga ggcgtggaag 1440aagttcgtgc tcgccaagag gaccaacggc gtctgctag 1479731386DNAOryza sativa Japonica 73atgccggagc agtcgatccc caaggaggcg gcgtaccaga tcatcaacga cgagctgatg 60ctggacggca acccgcggct gaacctcgcg tcgttcgtca ccacgtggat ggagcccgag 120tgcgacaagc tcatccaggc ctccgtcaac aagaactacg tcgacatgga cgagtacccc 180gtcaccaccg aactccagaa ccgatgtgtg aacatgattg cacacctctt caatgctcct 240ctaggggact ctgaaacggc cgtcggagtc ggcactgtcg gctcgtctga ggccatcatg 300ctcgccggtt tggccttcaa gaggaggtgg cagaacaaga tgaaggcagc cggcaagcca 360tgcgacaagc ctaacattgt caccggcgcc aatgtccaag tttgctggga gaagttcgcg 420cgatacttcg aggttgagct caaggaagtg aagctgagtg acggctacta cgtcatggac 480ccagctaagg ccgtggatat ggtcgacgag aacaccatct gcgtcgcggc gatcctcggg 540tcgacgctga acggggagtt cgaggacgtg aagctgctca acgatctgct caccaagaag 600aacgctgaaa caggctggga cacgccgatc cacgtggacg cggcgagcgg cgggttcatc 660gcgccgttcc tgtacccgga gctggagtgg gacttccggc tgccgctggt gaagagcatc 720aacgtgagcg ggcacaagta cggcctcgtc tacgccggga tcgggtggtg catctggagg 780agcaaggagg atctgcctga ggagctcatc ttccacatca actacctcgg cgccgaccag 840cccaccttca ccctcaactt ctccaagggt tccagccagg tcattgcaca gtattaccaa 900ctaatccgcc taggctttga ggggtacaag aacatcatgg agaactgcca ggagaacgcg 960atggtgctga agcaggggct ggagaagacg gggcggttca acatcgtgtc caaggacaac 1020ggcgtgccgc tggtggcctt ctccctcaag gacagcgccc ggcacaacga gttcgagatc 1080tccgacttcc tccgccgctt cggctggatc gtgccggcct acaccatgcc ccccgacgcg 1140cagcacgtca ccgtgctccg cgtcgtcatc cgcgaggact tcagccgcac gctcgccgag 1200cgcctcgtgc tcgacgtcga gaaggtgctg cacgagctcg acgcgctccc cgcccgcgtc 1260gtcgccaacg gcgacaaccc ggccgccgcg tcggcgagcg agagggagat ggagaagcag 1320cgcgaggtga tctccctctg gaagagggcc gtgctggcca agaagaagac caacggcgtc 1380tgctaa 1386741470DNAVitis vinifera 74atggttctct caaaaactgc ttctgagtcg gatgtctccg tgcactccac ctttgcctct 60cgctacgtga aagcttcgct tccgaggttc aagttgccgg agaactcgat cccgaaggag 120gcggcgtacc agatcataaa cgacgagctg atgcttgatg gcaatcctag gctaaacctg 180gcttcgtttg tgactacttg gatggagcct gaatgtgata agcttatgat ggctgccata 240aacaagaact atgtggacat ggatgaatac ccgaaccgat gcgtgaacat catagcccat 300ctattcaatg ctccattgga agactcagag gctgccgtgg gagtggggac ggtggggtcc 360tccgaggcga taatgctagc agggcttgct ttcaagagga agtggcagaa caagagaaag 420gctgagggga agccttatga caagcccaac atcgtcactg gtgctaatgt tcaggtgtgc 480tgggagaagt tcgcaaggta ctttgaggta gaactgaagg aagtgaaatt gagggacggc 540tactatgtaa tggacccaga gaaagctgtg gaaatggtgg atgaaaacac catctgtgtt 600gcggcaatct tgggctcaac tctcaatgga gaatttgaag atgttaagct cttgaacgat 660ctcttggtcg aaaagaacaa gcaaayagga tgggataccc caattcatgt tgatgcagct 720agtggtggat tcattgcacc attcctttat ccggaactgg agtgggactt ccgccttcca 780ttggtaaaga gcattaatgt tagtggccac aagtatggcc tcgtatatgc tggaattggg 840tgggttgttt ggaggagcaa agaggacttg cccgaagaac ttatcttcca cataaactac 900cttggagccg atcaaccaac cttcactctc aatttctcca aaggttctag tcaagtcatc 960gcccagtatt atcaactaat tcgcttgggt ttcgagggat accgaaatgt gatggagaat 1020tgccaggaaa atgctatggc gctgaaagaa ggactggaga agactgggcg ctttaacatc 1080atctccaagg acaatggggt ccctctggtg gccttctctc tcaaggacaa ttcatgccat 1140gatgagtttg aggttgcaga catgcttcgc cgctttggct ggattgtgcc tgcctacacc 1200atgccaccag atgctcagca tgttacagtg ctccgagttg tggttaggga agacttttca 1260cgcacccttg ctgagcgcct tgtatttgac atcaccaagg tgctccatga acttgatatg 1320ctcccagcaa agctcagtgc caagatttct gtcgaagaga aaaagcaaaa tggcacaatt 1380ctgaagaaat ccgtgatcga gacacagagg gaaatcaccg atgcttggaa gaaatttgtc 1440atggccaaga aaacgaacgg cgtttgttag 1470751488DNAVitis vinifera 75atggttctct caaaaactgc ttctgagtcg gatgtctccg tgcactccac ctttgcctct 60cgctacgtga aagcttcgct tccgaggttc aagttgccgg agaactcgat cccgaaggag 120gcggcgtacc agatcataaa cgacgaactg atgcttgatg gcaatcctag gctaaacctg 180gcttcgtttg tgactacttg gatggagcct gaatgtgata agcttatgat ggctgccata 240aacaagaact atgtggacat ggatgaatac ccggttacca ctgagcttca gaaccgatgc 300gtgaacatca tagcccatct attcaatgct ccattggaag actcagaggc tgccgtggga 360gtggggacgg tggggtcctc cgaggcgata atgctagcag ggcttgcttt caagaggaag 420tggcagaaca agagaaaggc tgaggggaag ccttatgaca agcccaacat cgtcactggt 480gctaatgttc aggtgtgctg ggagaagttc gcaaggtact ttgaggtaga actgaaggaa 540gtgaaattga gggacggcta ctatgtaatg gacccagaga aagctgtgga aatggtggat 600gaaaacacca tctgtgttgc ggcaatcttg ggctcaactc tcaatggaga atttgaagat 660gttaagctct tgaacgatct cttggtcgaa aagaacaagc aaacaggatg ggatacccca 720attcatgttg atgcagctag tggtggattc attgcaccat tcctttatcc ggaactggag 780tgggacttcc gccttccatt ggtgaagagc attaatgtta gtggccacaa gtatggcctc 840gtatatgctg gaattgggtg ggttgtttgg aggagcaaag aggacttgcc cgaagaactt 900atctttcaca taaactacct tggagccgat caaccaacct tcactctcaa tttctccaaa 960ggttctagtc aagtcatcgc ccagtattat caactaattc gcttgggttt cgagggatac 1020cgaaatgtga tggagaattg ccaggaaaat gctatggcgc tgaaagaagg actggagaag 1080actgggcgct ttaacatcat ctccaaggac aatggggtcc ctctggtggc cttctctctc 1140aaggacaatt catgccatga tgagtttgag gttgcagaca tgcttcgccg cttcggctgg 1200attgtgcctg cctacaccat gccaccagat gctcagcatg ttacagtgct ccgagttgtg 1260gttagggaag acttttcacg cacccttgct gagcgccttg tatttgacat caccaaggtg 1320ctccatgaac ttgatatgct cccagcaaag ctcagtgcca agatttctgt cgaagagaaa 1380aagcaaaatg gcacaattct gaagaaatcc gtgatcgaga cacagaggga aatcaccgat 1440gcttggaaga aatttgtcat ggccaagaaa acgaacggcg tttgttag 1488761431DNAVitis vinifera 76atgccggaga aatcgattcc aaaagaagca gcataccaga tagtacacga tgaattacta 60ttagatggtc ttcccaggct taacttggct acattcgtca ccacatggat ggaacctgag 120tgtgataagc tgatggcaga agccatcaac aagaattatg tagacatgga tgaataccca 180gtcacaactg agcttcagaa tcggtgcgtg aacatgattg caaaactttt caacgctcca 240tcagctgatc agacaaaaca agcagttgga gttgggacag tgggatcctc agaggcaatg 300atgttggccg gattggcatt caagaagaag tggcaaaaca agagaaaggc gcaaaagaaa 360ccttttgaca agcctaatat tgttactggt gccaatgttc aggtttgttg ggagaaattt 420gcaagatact ttgaagtgga gttgaaggaa gtgaaactaa gagaagggta ctatgtgatg 480gacccggtga aagcggtgga gatggtggat gagaacacca tctgtgttgc agctatcctg 540ggttctacct tcaatggtga atttgaagat gtcaagctct tgaacactct ccttactcaa 600aagaacaaga gaactgggtg ggacacccca attcatgtgg atgcagccag tggaggattt 660gtggctcctt ttttataccc agaactggaa tgggatttcc ggctaccttt ggtgaagagc 720atcaatgtca gcggccacaa atacggcctt gtctacgctg gagttgggtg ggccatttgg 780aggagcaaag aggaattgcc tgaagaactc atcttccaca taaactacct tggtggtgat 840gaacccacct tcaccctcaa cttctccaag ggcaatcagg ttattgcaca atactaccag 900ttcttgcgca tgggctttga gggatacaag aaagtgatga gcaactgcat ggaaagtgcg 960aggatacttc gagagggatt ggagaaaaca gggcgtttcc aaataatttc aaaggagaaa 1020ggagtacccg tcgtggcctt tgcattcaag ggtaacgatc gtaaaaactt ggcattcgga 1080ttatcaaaag ccttgaggaa ctacgggtgg attgttccag cttacacgat gccagccaat 1140gcggagaatg tcacggtcct tcgggtggtt gttcgtgagg actttgggcg gcagctggtt 1200gagaagttgc ttttccacat tggagttgcg ttgaaggagg tcacggatgc agcaagtagt 1260gttccaatga taagacttac agtggaaatg aaggcagatg agagtgaaat gaatgcaggt 1320gagggaactc tacacatacc ggcggcttca gtgcactgga agcatgataa acccgaaact 1380gttgacactc aagtgcccat aatggatggt aaaactaaag gggtttgctg a 1431771491DNANicotiana tabacum 77atggttctgt ccaagacagc gtcggaaagt gacgtctccg ttcactccac tttcgcctcc 60cgatatgttc gaacttctct tcccaggttt aaaatgccag agaattcaat accaaaggaa 120gcagcatatc agattataaa tgatgagctt atgttagatg gaaatccaag gctaaattta 180gcatctttcg ttacaacatg gatggagcca gaatgtaata cgttaatgat ggattccatt 240aacaagaact acgttgacat ggatgaatac cctgtaacca ctgagcttca gaatcgatgt 300gtaaatatga tagctcattt gtttaatgca ccacttggag atggagagac tgcagttgga 360gttggaactg ttggatcctc tgaagctatt atgcttgctg gattagcctt taagagaaaa 420tggcaaaata aaatgaaagc ccaaggcaag ccctttgata agcccaatat tgtcaccggt 480gctaatgtcc aggtgtgttg ggagaaattt gcaaggtatt ttgaagtgga gttgaaagaa 540gtaaaattga gtgatggata ctatgtgatg gaccctgaga aagctgtgga aatggtggat 600gagaatacca tttgtgttgc tgctatctta ggttcaacac tcaatggtga atttgaagat 660gttaagcgtt tgaatgacct tttgattgag aagaacaaag aaaccgggtg ggacactcca 720attcatgtgg atgcagcaag tggtggattt attgcaccat tcctttatcc agagcttgaa 780tgggacttta gattgccatt ggagaagagt attaatgtga gtggtcacaa atatggtctt 840gtctatgctg gtattggttg ggccatttgg aggaataagg aagacttgcc tgatgaactt 900attttccaca tcaattacct tggtgctgat caacctactt tcactctcaa cttctctaaa 960ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggttt tgagggttac 1020aagaatgtta tggagaattg tcaagaaaat gcaagggtat taagagaagg aattgaaaaa 1080agtggaagat tcaacataat ctccaaagaa attggagttc ccttagtagc attttctctt 1140aaagacaaca gtcaacacaa tgagttcgaa atttctgaaa ctcttagaag atttggatgg 1200attgttctgg catatactat gccaccaaat gctcaacatg tcacagttct cagagttgtc 1260attagagaag atttctcccg cacactagcg gagcgactgg taatagacat tgaaaaagtc 1320ttccacggag tagacacact tccggcgagg gtcaacgcta agctagccgt ggccgaggcg 1380aatggcagcg gcgtgcataa gaaaacagat agagaagtgc agctagagat tactactgca 1440tggttgaaat ttgttgctga taagaagaag aagactaatg gagtttgtta a 1491781491DNANicotiana tabacum 78atggttctgt ccaagacagc gtcggaaagt gacgtctcca tccactccac tttcgcttcc 60cgatatgttc gtacttctct tccgaggttt aagatgccag agaattcgat accaaaggaa 120gcagcatatc aaatcataaa tgatgagctt atgttagatg gaaatccaag actaaattta 180gcatcttttg tgacaacatg gatggaacca gagtgtaaca aactgatgat ggattccatt 240aacaagaatt acgttgacat ggatgaatac cctgtaacca ctgaacttca gaatcgatgt 300gtaaacatga tagctcattt gtttaacgca ccacttggag atggagagac tgcagttgga 360gttggaactg ttggatcctc tgaggctatt atgcttgctg gattagcttt caagagaaaa 420tggcaaaata aaatgaaagc ccaaggcaag ccctgtgaca agcccaatat tgtcactggt 480gccaatgtcc aggtgtgttg ggagaaattt gcaaggtatt ttgaagtgga gctaaaggaa 540gtaaagttga gtgatggata ctatgtgatg gaccctgaga aagctgtgga aatggtggat

600gagaacacaa tttgtgtagc tgctatcttg ggttccacac tcaatggtga atttgaagat 660gttaagcgct tgaatgacct cttgattgag aagaacaaag aaaccgggtg ggacactcca 720attcatgtgg atgcagcaag tggtggattt attgcaccat tcctttatcc agagcttgaa 780tgggacttta gattgccatt ggtgaagagt ataaacgtga gtggtcacaa atatggtctt 840gtttatgctg gtattggttg ggccatttgg aggaataagg aagacttacc tgacgaactt 900atcttccaca ttaattatct tggtgctgat caacctactt tcactctcaa cttctctaaa 960ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggttt tgagggttac 1020aagaatgtta tggagaattg tcaagaaaat gcaagggtac taagagaagg acttgaaaaa 1080agtggaagat tcaacataat atccaaagaa attggagttc cattagtagc tttctctctt 1140aaagacaaca gtcaacacaa tgagttcgaa atttctgaaa ctcttagaag atttggatgg 1200attattcctg catatactat gccaccaaat gctcaacatg tcacagttct cagagttgtc 1260attagagaag atttctcccg tacactcgcc gagcgactgg taatagacat tgaaaaagtc 1320ctccacgagc tagacacact tccggcgagg gtcaacgcta agctagccgt ggccgaggcg 1380aatggcagcg gcgtgcataa gaaaacagat agagaagtgc agcttgagat tactactgca 1440tggaagaaat ttgttgctga taagaagaag aagactaacg gagtttgtta a 1491791491DNANicotiana tabacum 79atggttctgt ccaagacagc gtcggaaagt gacgtctcca tccactccac tttcgcttcc 60cgatatgttc gtacttctct tccgaggttt aagatgccag agaattcgat accaaaggaa 120gcagcatatc aaatcataaa tgatgagctt atgttagatg gaaatccaag actaaattta 180gcatcttttg tgacaacatg gatggaacca gagtgtaaca aactgatgat ggattccatt 240aacaagaatt acgttgacat ggatgaatac cctgtaacca ctgaacttca gaatcgatgt 300gtaaacatga tagctcattt gtttaacgca ccacttggag atggagagac tgcagttgga 360gttggaactg ttggatcctc tgaggctatt atgcttgctg gattagcttt caagagaaaa 420tggcaaaata aaatgaaagc ccaaggcaag ccctgtgaca agcccaatat tgtcactggt 480gccaatgtcc aggtgtgttg ggagaaattt gcaaggtatt ttgaagtgga gctaaaggaa 540gtaaagttga gtgatggata ctatgtgatg gaccctgaga aagctgtgga aatggtggat 600gagaacacaa tttgtgtagc tgctatcttg ggttccacac tcaatggtga atttgaagat 660gttaagcgct tgaatgacct cttgattgag aagaacaaag aaaccgggtg ggacactcca 720attcatgtgg atgcagcaag tggtgaattt attgcaccat tcctttatcc agagcttgaa 780tgggacttta gattgccatt ggtgaagagt attaacgtga gtggtcacaa atatggtctt 840gtttatgctg gtattggttg ggccatttgg aggaataagg aagacttacc tgacgaactt 900atcttccaca ttaattatct tggtgctgat caacctactt tcactctcaa cttctctaaa 960ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggttt tgagggttac 1020aagaatgtta tggagaattg tcaagaaaat gcaagggtac taagagaagg acttgaaaaa 1080agtggaagat tcaacataat atccaaagaa attggagttc cattagtagc tttctctctt 1140aaagacaaca gtcaacacaa tgagttcgaa atttctgaaa ctcttagaag atttggatgg 1200attattcctg catatactat gccaccaaat gctcaacatg tcacagttct cagagttgtc 1260attagagaag atttctcccg tacactcgcc gagcgactgg taatagacat tgaaaaagtc 1320ctccacgagc tagacacact tccggcgagg gtcaacgcta agctagccgt ggccgaggcg 1380aatggcagcg gcgtgcataa gaaaacagat agagaagtgc agcttgagat tactactgca 1440tggaagaaat ttgttgctga taagaagaag aagactaacg gagtttgtta a 1491801503DNAOryza sativa 80atggttctga cgcacgtcga ggcggtggag gagggcagcg aggcggcggc cgccgtgttc 60gcgtcgaggt acgtgcagga cccggtgccg aggtacgagc tcggcgagag gtcgatatcc 120aaggacgccg cgtaccagat cgtccacgac gagctcctcc tggacagcag cccgcgcctg 180aacctggcgt ccttcgtcac cacctggatg gagcccgagt gcgacaggct catcctcgag 240gccatcaaca agaactacgc cgacatggac gagtaccccg tcaccaccga gctccagaac 300cggtgcgtga acatcatagc gaggctgttc aatgcgccgg tgggcgacgg cgagaaggcg 360gtcggggtgg gcacggtggg gtcgtcggag gccataatgc tggccgggct ggcgttcaag 420cggcggtggc agaaccggcg gaaggcggcg gggaagcccc acgacaagcc caacatcgtg 480acgggggcca acgtgcaggt gtgctgggag aagttcgcgc gctacttcga ggtggagctc 540aaggaggtga agctgaccga aggctgctac gtgatggacc ccgtcaaggc cgtggacatg 600gtcgacgaga acaccatctg cgtcgccgcc atcctcggct ccaccctcac cggcgagttc 660gaggacgtca ggcgcctcaa cgacctcctc gccgccaaga acaagcggac gggttgggac 720acgccgatcc acgtcgacgc ggcgagcggc gggttcatcg cgccgttcat ctacccggag 780ctggagtggg acttccggct gccgctggtg aagagcatca acgtcagcgg ccacaagtac 840gggctcgtct acgccggcgt cgggtgggtc atctggcgca acaaggagga cctccccgag 900gagctcatct tccacatcaa ctacctcggc gccgaccagc caaccttcac gctcaacttc 960tccaaagggt ccagtcagat tattgcgcaa tattaccagt ttcttcgact cggatttgag 1020gggtacaaga gcgtgatgaa gaactgcatg gagagcgcga ggacgctccg ggagggcctg 1080gagaagacgg ggcggttcac catcatctcc aaggaggagg gcgtgccgct ggtggccttc 1140acgttcaagg acggcgccgg cgcgcaggcc ttcaggctgt cgtcgggcct gcgccggtac 1200gggtggatcg tgccggcgta cacgatgccg gcggcgctgg agcacatgac ggtgctccgc 1260gtcgtcgtcc gggaagactt cggccggccg ctcgccgagc ggttcctgtc ccacgtcagg 1320atggccctgg acgagatgga cctcgccgcc agggcccccg tgcccagggt gcagctcacc 1380atcgagctcg gccccgcccg gaccgccggc gaggaggcct cgatcagggt ggtcaagagc 1440gaggccgtgc ccgtgcgcaa gagcgtcccg ctcgtcgccg gcaaaaccaa gggcgtttgc 1500tag 1503811485DNACitrus sinensis 81atggttcttt caaagacatt ttccgaatcc gatgagtcaa ttcactccac ctttgcctct 60cgctacgtcc gaaactctct tcctcggttc acgatgccgg agaactcgat accgaaggag 120gcggcgtacc agatcataaa cgacgagctg atgctcgacg ggaatccaag attgaacttg 180gcttccttcg tcacaacgtg gatggagcct gaatgtgaca agcttatgat ggctgccatt 240aacaagaact acgttgacat ggatgagtat cctgtcacca ccgagcttca gaatcgatgt 300gtgaacatta tagcccgact gttcaatgct ccactggagg actccgagac ggctgtcgga 360gttggaaccg taggatcatc ggaagccata atgttggccg gccttgcatt caaaagaaag 420tggcagaaca agcgcaaggc tgaaggaaag ccctttgaca aacccaacat tgtcactgga 480gcgaacgttc aggtctgctg ggagaaattt gcaaggtatt ttgaggtgga gttgaaggaa 540gtgaagctgt cggaaggcta ctacgtgatg gacccagcca aagctgtaga aatggtggat 600gaaaacacta tctgtgttgc tgctattctg ggttctactc tcaatggaga atttgaagat 660gtcaagctct tgaacgacct tttgacagag aagaacaagg aaacaggatg ggatactcca 720attcatgttg atgctgctag tggtggcttc attgcaccat ttttgtaccc agagcttgaa 780tgggacttcc gcttgccgct ggtgaagagt attaatgtta gtggccacaa gtacgggctt 840gtgtatgctg gtattggttg ggttgtttgg agaaacaaag aagacttgcc tgaagaactc 900atcttccaca tcaattacct aggagctgat caacccacct tcaccctcaa tttttctaaa 960ggttctagcc aagtaattgc tcagtactac caactaatcc gcttaggttt tgagggatac 1020cgcaatgtga tggagaattg tcacgaaaat gcaatggtgc tgaaagaagg actagagaaa 1080accggccgct tcaacattgt gtcaaaggac gaaggggttc cgttggtggc attttctctc 1140aaggacaaca aacgccacga tgaattcgaa gtggccgaat tgctgcgccg ttttggttgg 1200attgtgccag cgtacaccat gcccgctgac gctcagcaca tcaccgtgtt acgtgtcgtc 1260atcagggaag acttctctcg cacacttgcc gagcgccttg tgctcgacat cacaaaagtg 1320cttcatgagc ttgattcgct tccttcaaaa gtgcttgtac ctgcttctga acagaatgga 1380aggaatggaa agaagactga aattgagact cagagggaag tcactactta ctggaggaaa 1440ttcgtctccg aaaggaaagc taataacaag aataaaattt gttaa 1485821479DNAOryza sativa Japonica 82atggtgctct cccacggcgt gtcgggctcc gatgagtccg tccactccac gttcgcctcc 60cgctacgtcc gcacctccct ccccaggttc cggatgccgg agcagtcgat ccccaaggag 120gcggcgtacc agatcatcaa cgacgagctg atgctggacg gcaacccgcg gctgaacctc 180gcgtcgttcg tcaccacgtg gatggagccc gagtgcgaca agctcatcca ggcctccgtc 240aacaagaact acgtcgacat ggacgagtac cccgtcacca ccgaactcca gaaccgatgt 300gtgaacatga ttgcacacct cttcaatgct cctctagggg actctgaaac ggccgtcgga 360gtcggcactg tcggctcgtc tgaggccatc atgctcgccg gtttggcctt caagaggagg 420tggcagaaca agatgaaggc agccggcaag ccatgcgaca agcctaacat tgtcaccggc 480gccaatgtcc aagtttgctg ggagaagttc gcgcgatact tcgaggttga gctcaaggaa 540gtgaagctga gtgacggcta ctacgtcatg gacccagcta aggccgtgga tatggtcgac 600gagaacacca tctgcgtcgc ggcgatcctc gggtcgacgc tgaacgggga gttcgaggac 660gtgaagctgc tcaacgatct gctcaccaag aagaacgctg aaacaggctg ggacacgccg 720atccacgtgg acgcggcgag cggcgggttc atcgcgccgt tcctgtaccc ggagctggag 780tgggacttcc ggctgccgct ggtgaagagc atcaacgtga gcgggcacaa gtacggcctc 840gtctacgccg ggatcgggtg gtgcatctgg aggagcaagg aggatctgcc tgaggagctc 900atcttccaca tcaactacct cggcgccgac cagcccacct tcaccctcaa cttctccaag 960ggttccagcc aggtcattgc acagtattac caactaatcc gcctaggctt tgaggggtac 1020aagaacatca tggagaactg ccaggagaac gcgatggtgc tgaagcaggg gctggagaag 1080acggggcggt tcaacatcgt gtccaaggac aacggcgtgc cgctggtggc cttctccctc 1140aaggacagcg cccggcacaa cgagttcgag atctccgact tcctccgccg cttcggctgg 1200atcgtgccgg cctacaccat gccccccgac gcgcagcacg tcaccgtgct ccgcgtcgtc 1260atccgcgagg acttcagccg cacgctcgcc gagcgcctcg tgctcgacgt cgagaaggtg 1320ctgcacgagc tcgacgcgct ccccgcccgc gtcgtcgcca acggcggcga cgccgccgcc 1380gcgtcggcga gcgagaggga gatggagaag cagcgcgagg tgatctccct ctggaagagg 1440gccgtgctgg ccaagaagaa gaccaacggc gtctgctaa 1479831482DNABrassica juncea 83atggttttgt ctaagacagc ttctggaact gatgtttccg tccattcaac ttttgcttct 60cgttatgtcc gcaactcgct ccctcgattc gagatgcctg agaactccat cccgaaggaa 120gcagcgtacc agatcatcaa cgatgagcta atgctcgacg gtaaccctag gctaaatcta 180gcctccttcg tgactacgtg gatggagcca gagtgtgaca agctcatgat ggaatctatc 240aacaagaact acgttgacat ggacgagtac cctgtcacca ccgagcttca gaaccgatgt 300gtcaacatga ttgcgcgtct ctttaacgcg ccgctaggtg acggtgaggc tgcggttggt 360gtcggcaccg tgggatcgtc tgaggcgatt atgttggccg ggttggcttt taagagacag 420tggcagaaca agcgtaaggc ccaagggctt ccttatgata agcctaatat cgtaaccgga 480gctaatgttc aggtttgctg ggagaaattc gcaaggtatt tcgaggtgga acttaaggaa 540gtgaagctga gagaaggata ctacgtgatg gaccctgaaa aggcagtcga aatggtagac 600gagaacacca tttgtgtcgc agccatcctc ggttcgacgc taaccggaga attcgaagac 660gttaagctcc tcaatgacct cctagtcgag aaaaacaagc aaaccggatg ggatactggg 720aatcacgtgg acgcagcaag tggtgggttt attgcaccgt tcttgtatcc ggagctggag 780tgggatttcc ggttaccatt ggttaagagc ataaatgtta gtggccacaa atacggtctg 840gtttatgctg gaatcggttg ggttgtgtgg agaaccaaat ctgatttgcc tgatgaactt 900atcttccaca tcaattatct tggcgctgat caacccacct tcactctcaa cttctccaag 960ggttcgagtc aagtgattgc tcagtactac caactgattc gtcttggatt cgagggatat 1020cgtaacgtga tggataattg tcgtgaaaac atgatggtcc taagagaagg actagagaaa 1080acgggacgtt tcaacattgt ctccaaagaa aacggtgttc cgttagtggc gttttctcta 1140aaagacagta gccgccacaa tgagttcgaa gtggcggaaa ctctccgccg ctttggatgg 1200atcgttccgg cctacacggt gccagcggat gcagaacatg tcaccgtcct ccgagtggtg 1260attcgagaag atttctctcg aaccttagct gagagattgg ttgcagactt tgagaaggtt 1320cttcacgagc tcgatacact tccggccagg gttcgcgcca agatggctaa tggaaaagct 1380aaagttgtta aacagacgga ggaggagacg acgagggaag ttacggcata ttggaagaag 1440tttgtggaga caaagaagac taaccagaac aagatttgct aa 1482841482DNABrassica juncea 84atggttttgt ctaagacagc ttcggaatct gatgtttcaa tccattcaac ttttgcttct 60cgttacgtcc gcacctctct cccacgattt gagatgcctg agaactcgat cccaaaggaa 120gcagcgtacc aaatcatcaa cgacgagcta atgctcgacg gtaacccaag gctaaatcta 180gcctccttcg tgaccacgtg gatggagcca gagtgcgaca agctcatgat ggaatccatc 240aacaagaact acgtcgacat ggacgagtac cctgtcacca ccgagcttca gaaccgatgc 300gtcaacatga tcgcgcgtct cttcaacgcg ccgctaggtg acggcgaggc tgcggttggc 360gtcggcaccg tgggatcgtc ggaggcgatt atgttggctg gattggcctt taagagacag 420tggcagaata agcgtaaggc tcaagggctt ccttatgata agcccaatat cgttaccgga 480gccaatgttc aggtttgctg ggagaagttt gcaaggtatt tcgaggtgga gcttaaagaa 540gttaagctaa gagaaggata ctacgtgatg gacccagaga aggcggtcga aatggtagac 600gagaacacaa tctgtgttgc agccatcctc ggttccactc taacaggaga attcgaagac 660gttaagctcc ttaacgacct cctagtcgag aaaaacaagc aaaccggatg ggatacgggg 720atccatgtgg acgcagcgag tggtgggttt attgctcctt tcttgtatcc agagctggag 780tgggatttcc ggttaccatt ggttaagagc ataaatgtga gtggtcacaa atacggtttg 840gtttacgctg gaatcggttg ggttgtatgg agaaccaaat ccgatttgcc tgatgaactt 900atcttccata tcaactatct tggcgctgac caaccgacct tcactctcaa cttctccaaa 960ggttcaagtc aagtgattgc tcagtactac cagctgattc gtcttggatt cgagggatat 1020cgcaacgtga tggataattg ccgtgaaaat atgatggtcc taagagaagg attagagaag 1080acaggacgtt tcaacatagt ctcaaaagaa aacggtgttc cgttagtggc attttcttta 1140aaagacagta gtcgccacga cgagttcgaa gtggccgaga ctctccgtcg ctttgggtgg 1200attgttccgg cctacacgat gcccgcggat gctcaacatg tcaccgtcct ccgagtggtg 1260attcgagaag atttctctcg aactttggct gagagattgg tcgcagactt cgagaaggtt 1320ctccacgagc tcgatacgct tccggcgagg gttcaggcca agatggctaa cggaaacgct 1380aacggtgtta agaagacgga agaggaaacg acgagggaag ttactgcgta ttggaagaag 1440tttgtggaag caaagaagag taacaagaac aggatttgct aa 1482851485DNABrassica juncea 85atggttctaa gtcgagcggc caccgaaagt ggcgaaaatg tttgctcgac gttcggatct 60cgctatgtcc gcaccgcact gcccaagcat aagattggtg agagctcgat cccgaaggag 120gctgcgtatc agatcataaa agatgagctg atgcttgatg gtaacccgag gctgaacctg 180gcttcgtttg tgacgacatg gatggagcca gagtgtgaca aactcatcat ggaatctatc 240aacaagaact acgtcgacat ggacgagtac cctgtcacta ccgaactcca gaaccgatgt 300gtaaacatga tagctcggct gttcaatgcg ccgcttgagg aaactgagac cgccatggga 360gtaggcactg ttgggtcttc ggaagccatc atgttagccg gattggcctt caaaaggaat 420tggcagaaca aacgcaaagc tgagggtaaa ccctatgaca aacccaacat tgtcaccgga 480gccaatgttc aagtgtgctg ggagaaattc gctaggtact tcgaggtgga gctaaaagaa 540gtgaagctta gtgaaggtta ctacgtgatg gatccggata aagcagctga aatggtagac 600gagaatacaa tctgtgttgc tgccatactt ggttctacac tcaacggtga gttcgaagac 660gtcaagcgcc ttaatgactt gctggtcaag aaaaacgaag agactggctg gaacactcca 720atccacgttg acgcagcaag tggaggcttc atagctccgt ttatctaccc tgagttggaa 780tgggacttta ggcttccttt ggtgaagagt atcaatgtga gtggtcataa gtatgggctg 840gtctatgctg gtattggctg ggtcgtgtgg aggacacaac aggatttgcc tgatgagctc 900atctttcata ttaactatct tggtgctgat caacccacat ttactctcaa tttctccaag 960ggatcgagcc aaattattgc tcaatattat cagctcattc gtcttggctt cgagggctac 1020aagaacgtga tggagaactg cagagagaac atggtggttc tgagagaagg gatcgagaaa 1080acagagcgtt tcaacatagt ctcaaaggag gtaggagttc cactcgtagc cttctccctc 1140aaggaccaca gtttccacaa cgagttcgaa atctcagaga tgctacgccg tttcggctgg 1200attgtcccgg cttacacaat gcctgcggat gcgcaacaca tcacagttct gcgtgttgtc 1260atcagggaag atttctcaag aacacttgcg gagagacttg tggctgatat tgtgaaggtg 1320cttcacgagc tcgacacctt gccttccaag atatctaaga agatgggagc agaggatttc 1380ggaaacgtga aagggaagaa ggtggatagg gatgttctga tggaagtcat tgttggatgg 1440aggaagtttg tgaaggacag gaagaagatg aatggtgtgt gttga 1485861494DNAOryza sativa Japonica 86atggtgctct cgcacgcgag ctccggccgg gacgacgccg tgcgctgcac cttcgcgacg 60cgctacgcct gcgagacgct gccgcggttc aggatgccgg agcagtcgat cccgagggag 120gcggcgtacc agatcatcaa cgacgagctg atgctggacg ggaacccgcg gctgaacctg 180gcgtccttcg tcaccacgtg gatggagccc gagtgcgaca agctcatcat ggactccgtt 240aacaagaact acgtcgacat ggacgagtac cctgtcacca cggagctcca gaaccgttgt 300gtgaatatga tagctcacct gttcaatgca ccaatcaagg aggatgaaac agctattgga 360gttgggacgg tgggatcctc agaagcaatt atgcttgcag gactggcatt caagaggaag 420tggcaaaaca aacggaagga acaggggaag ccatgtgaca aacccaacat tgttactggt 480gctaatgttc aggtttgctg ggagaaattt gccagatatt ttgaagtaga actgaaggag 540gttaagctca gtgaaggata ctatgtcatg gatcctgtaa aggctgttga aatggtggat 600gagaacacta tatgcgttgc ggccatcttg ggctctactc tcactggaga gtttgaggat 660gttaagttat tgaataatct cctaacagaa aagaataagg aaactgggtg ggatgtgcca 720attcatgttg atgcagcaag tggaggattt atagcacctt ttctataccc tgagcttgaa 780tgggacttca ggctaccact ggtgaagagc atcaatgtca gtgggcacaa gtatggcctt 840gtgtatccag gtgttggttg ggtcatttgg cgaagcaaag aggatttgcc tgaagaactc 900attttccata taaactatct ggggacagac cagccgacgt tcactctgaa cttctccaaa 960ggttccagcc agataatcgc acagtactat caactaatac gcctgggatt cgagggatac 1020aagaacatca tgcagaattg catggagaac acagcaatac taagggaagg catagaggcg 1080actggtcgat tcgaaatcct ctccaaggag gccggtgtgc ccttggtggc gttctcgctc 1140aaggacagcg gcaggtacac cgtgttcgac atctccgagc acctgaggag gttcggctgg 1200atcgtgccgg cgtacaccat gccggccaac gccgagcacg tcgccgtcct ccgcgtcgtc 1260atcagggagg acttcagccg gagcctcgcc gagcggctcg tctcggacat cgtcaagatc 1320ctgcacgagc tggacgccca ttcggcccag gtgctgaaga tctccagcgc catcgcgaag 1380cagcaatcgg gcgacgatgg cgtggtcacc aagaagagcg tcctggagac cgagagggag 1440atcttcgcgt actggaggga ccaggtgaag aagaagcaga ccggaatctg ctag 1494871473DNAHordeum vulgare 87atggtggtga ccgtggcagc gacggggccg gacacggccg agacgctgca ctccaccacc 60ttcgcctccc gctacgtccg cgaccagctc ccccggtacc ggatgccgga gaactcgatc 120cccaaggagg cggcgtacca gatcatcagc gacgagctga tgctggacgg caacccgcgg 180ctgaacctgg cgtccttcgt caccacctgg atggagcccg agtgcggcaa gctcatcatg 240gactccgtca acaagaacta cgtcgacatg gacgagtacc ccgtcaccac cgagctccag 300gaccgttgcg taaacatgat agctcacttg ttcaatgcac cgatcggcga ggacgagaca 360gctatcggag tctcgacggt ggggtcttcg gaagcaatca tgcttgcagg cctggcgttc 420aagaggaagt gggcgaacaa aatgaaggag caggggaagc catgcgacaa acctaacatt 480gttactggtg caaatgttca ggtttgctgg gagaaatttg ctaggtattt tgaagtggaa 540ttgaaggagg tcaagttgac tgaagggtac tatgtcatgg atcctaagaa ggctgttgaa 600atggtggatg agaacactat atgtgtcgcc gccatcctgg gatctactct cactggagag 660tacgaagatg tcaaactgtt gaatgacctt cttgtggaga agaacaagga aacagggtgg 720aacgtgccga tccatgttga tgctgccagc ggaggattta tcgctccgtt tcttcagcct 780gagcttgaat gggacttcag gctaccattg gtgaagagca tcaacgttag tgggcacaag 840tatggccttg tgtaccctgg tgttggatgg gtcatctggc ggagcaagga cgatttgccc 900gaagaactca ttttccacat aaactatcta ggagcagatc agcccacatt cacgctcaac 960ttctccaagg gtcagcagat catcgcgcaa tactatcagc tcatccgcct cggcttcgag 1020gggtacaagc acatcatgga gaactgcaag ctgaacgcgg cggtgctgaa ggagggcatc 1080gacgcgacgg ggcggttcga cgtgctgtcc aaggcggacg gcgtgccgct ggtggccatc 1140cggctcaagg acagcaccaa cttcagcgtg ttcgacatct cggagaacct gaggcggttc 1200gggtggatcg tgccggcgta caccatgccc gccgacgcgg agcatgtggc cgtgctccgc 1260atagtcatcc gggaggactt caaccggagc ctcgcgcagc ggctcctcgc cgacatcaac 1320aagatcatcg gcgagctgga cgcgcacgcc gtccacgcca tcaagctctc caccgccgcc 1380gctggtgggg acggcgcgag taagagcgcg gtcgacgccg ccaccgaggc cttcaaggac 1440ctggcgggga agaagaaggc cggagtatgc tga 1473881479DNAOryza sativa Japonica 88atggtgctct ccaaggccgt ctccgagagt gacatgtccg tgcactccac cttcgcctcc 60cgctacgtcc gcgcctccct cccaaggtac cggatgccgg agaactcgat cccgaaggag 120gcggcgtacc agatcatcaa cgacgagctg atgctggacg gcaacccgcg gctgaacctg 180gcgtcgttcg tcaccacgtg gatggagccc gagtgcgaca agctcatcat ggccgccatc 240aacaagaact acgtcgacat ggacgagtac cccgtcacca ccgagctcca gaaccggtgc

300gtgaacatga tcgcgcacct gttccacgcg ccgctcgggg aggacgagac ggcggtgggc 360gtgggcacgg tgggttcgtc ggaggccatc atgctggccg ggctggcctt caagcggcgg 420tggcagaaca agcgcaaggc cgaggggaag ccgttcgaca agcccaacat catcaccggc 480gccaacgtgc aggtgtgctg ggagaagttc gcccgctact tcgaggtgga gctcaaggag 540gtgaagctcc gcgacggcta ctacgtcatg gaccccgaga aggccgtcga catggtcaac 600gagaacacca tctgcgtcgc cgccatcctc ggctccaccc tcaacggcga gttcgaggac 660gtcaagctac tcaacgacct cctcgacaag aagaacaagg agactgggtg ggagacgccg 720atccacgtgg acgcggcgag cggcgggttc atcgcgccgt tcctgtaccc ggagctggag 780tgggacttcc ggctgccgtg ggtgaagagc atcaacgtga gcggtcacaa gtacgggctc 840gtctacgccg gcatcggctg gtgcatctgg cgcaacaagg aggacctgcc cgaggagctc 900atcttccaca tcaactacct cggcaccgac cagccaacct tcaccctcaa cttctccaag 960ggctccagcc aggtcatcgc ccagtactac cagctcatcc gccacggctt cgaggggtac 1020aggaacatca tggagaactg ccacgagaac gcgatggtgc tgaaggaagg gctggtgaag 1080acggggaggt tcgacatcgt gtccaaggac gaaggggtgc cgctggtggc gttctcgctc 1140aaggaccgga gccggcacga cgagttcgag atctccgaca tgctgcgccg cttcggctgg 1200atcgtgccgg cgtacaccat gccgcccgac gcccagcacg tcacggtgct ccgcgtggtc 1260atccgggagg agttcagccg caccctcgcc gagcgcctcg tcctcgacat cgagaaggtg 1320atgtaccagc tcgacgcgct cccctccagg ctcatgcccc ccgtgccgcc ggcgccgctg 1380ctggtggtcg ccaagaagtc ggagctcgag acgcagcggt cggtgacgga ggcgtggaag 1440aagttcgtgc tcgccaagag gaccaacggc gtctgctag 1479891485DNAArabidopsis thaliana 89atggtactcg caaccaactc tgactccgac gagcatttgc attccacttt tgcttctaga 60tatgtccgtg ctgttgttcc caggttcaag atgcctgacc attgcatgcc caaagatgct 120gcttatcaag tgatcaatga tgagttgatg cttgatggta atcccaggct taacctagcc 180tcctttgtca ccacttggat ggaacctgag tgtgacaaac tcatcatgga ttctgtcaat 240aagaactatg ttgatatgga tgaatatcct gtcaccactg agctccagaa ccggtgtgta 300aatatgatag caaacttttt ccatgctccc gttggagaag acgaggctgc tattgggtgt 360ggaactgttg gttcatctga ggctataatg cttgctggtt tggctttcaa aaggaaatgg 420caacatagga gaaaagctca gggtctacct attgataagc ctaacattgt cactggagcc 480aatgttcagg tgtgctggga gaagtttgca aggtactttg aggtagagct caaagaggtg 540aaactaagtg aagactacta tgttatggat ccagctaaag ctgtagagat ggtggatgag 600aataccatct gtgttgcagc aattctagga tctacactta ctggagagtt tgaggacgtt 660aagcaattga acgatctctt agctgagaaa aacgcagaga caggatggga aactcctatt 720catgttgatg cagccagtgg aggattcatt gctcctttcc tctaccctga tcttgaatgg 780gactttaggc ttccatgggt gaagagtatt aacgtcagtg gtcacaagta tggacttgtg 840tatgcaggag ttggttgggt tgtctggaga acaaaagatg atttgccaga ggaacttgtc 900ttccacatca actacttggg agctgatcaa cccactttca ctctcaactt ctcaaaaggg 960tcgagccaaa tcattgctca gtactatcag tttatccgac taggctttga gggatacaag 1020aacataatgg aaaactgcat ggataacgca aggaggctaa gagaaggaat agagatgaca 1080gggaagttca acattgtgtc caaagatatt ggcgtgccac tagtggcatt ctctctcaaa 1140gacagtagca agcacacggt gtttgagatc gcagagtctt tgagaaaatt cgggtggatc 1200ataccggctt acactatgcc tgcagatgca cagcacattg ctgtgctcag agttgtgata 1260agagaagact ttagccgagg ccttgcagat agactcatca cacatatcat tcaggtgctg 1320aaagagattg aagggcttcc tagcaggatt gcacatcttg ctgcggctgc agcggttagt 1380ggtgatgatg aagaagttaa agtgaagact gccaagatgt ccttggagga tatcactaag 1440tattggaaac gccttgtgga acacaagaga aatattgtct gctaa 1485901491DNANicotiana tabacum 90atggttctgt ccaagacagc gtcggaaagt gacgtctcca tccactccac tttcgcttcc 60cgatatgttc gaacttctct tcccaggttt aagatgccag agaattcaat accaaaggaa 120gcagcatatc agattataaa tgatgagctt atgttagatg gaaatccaag gctaaattta 180gcatctttcg ttacaacatg gatggagcca gaatgtaata cgttaatgat ggattccatt 240aacaagaact acgttgacat ggatgaatac cctgtaacca ctgagcttca gaatcgatgt 300gtaaatatga tagctcattt gtttaatgca ccacttggag atggagagac tgcagttgga 360gttggaactg ttggatcctc tgaagctatt atgcttgctg gattagcctt taaaagaaaa 420tggcaaaata aaatgaaagc ccaaggcaag ccctttgata agcccaatat cgtcaccggt 480gctaatgtcc aggtgtgttg ggagaaattt gcaaggtatt ttgaagtgga gttgaaagaa 540gtaaaattga gtgatggata ctatgtgatg gaccctgaga aagctgtgga aatggtggat 600gagaatacca tttgtgttgc tgctatctta ggttcaacac tcaatggtga atttgaagat 660gttaagcgtt tgaatgacct tttgattgag aagaacaaag aaaccgggtg ggacactcca 720attcatgtgg atgcagcaag tggtggattt attgcaccat tcctttatcc agagcttgaa 780tgggacttta gattgccatt ggtgaagagt attaatgtga gtggtcacaa atatggtctt 840gtctatgctg gtattggttg ggccatttgg aggaataagg aagacttgcc tgatgaactt 900attttccaca tcaattacct tggtgctgat caacctactt tcactctcaa cttctctaaa 960ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggttt tgagggttac 1020aagaatgtta tggagaattg tcaagaaaat gcaagggtat taagagaagg aattgaaaaa 1080agtggaagat tcaacataat ctccaaagaa attggagttc ccttagtagc attttctctt 1140aaagacaaca gtcaacacaa tgagttcgaa atttctgaaa ctcttagaag atttggatgg 1200attgttcctg catatactat gccaccaaat gctcaacatg ttacagttct cagagttgtc 1260attagagaag atttctcccg cacactagcg gagcgactgg taatagacat tgaaaaagtc 1320ctccacgagc tagacacact tccggcgagg gtcaacgcta agctagccgt ggccgaggcg 1380aatggcagcg gcgtgcataa gaaaacagat agagaagtgc agctagagat tactactgca 1440tggaagaaat ttgttgctga taagaagaag aagactaatg gagtttgtta a 1491911485DNAArabidopsis thaliana 91atggttttga caaaaaccgc aacgaatgat gaatctgtct gcaccatgtt cggatctcgc 60tatgttcgca ctacacttcc caagtatgag attggtgaga attcgatacc gaaagacgct 120gcgtatcaga tcataaaaga tgagctgatg cttgatggta acccaaggct taacctagct 180tcttttgtga ctacatggat ggaaccagag tgtgacaaac tcatcatgga ctctatcaat 240aagaactacg ttgatatgga tgagtaccct gtcacaactg agctccagaa ccgatgtgta 300aacattatag ctcgactgtt caatgcgcca ctcgaggaat ctgagacggc ggtgggagta 360gggacagttg gttcttcaga agccatcatg ttagccggat tggccttcaa aagaaaatgg 420cagaacaaac gcaaggctga gggtaaaccc tatgacaaac ccaacattgt caccggagcc 480aatgttcaag tttgctggga gaaattcgct cggtacttcg aggtggagct aaaggaagta 540aacctaagtg aaggttacta cgtgatggat ccagacaaag cagcagaaat ggtagacgag 600aacacaatct gtgtcgcagc catattggga tccacactca acggtgagtt cgaagacgtg 660aaacgtctca atgacttgct agtcaagaaa aacgaggaga ctggttggaa cacaccgatc 720cacgtggatg cagcaagtgg agggttcata gctccgttta tctatcctga attagaatgg 780gactttaggc ttcctttggt taagagcatc aacgtgagtg gtcacaagta tggactagtc 840tatgctggta ttggttgggt cgtgtggagg gcagcagagg atttacctga agagcttatc 900tttcatatta attatcttgg tgctgatcaa cccactttca ctctcaattt ctccaaggga 960tcgagccaaa ttattgctca atactaccag ctcattcgtc ttggattcga ggggtacaaa 1020aatgtgatgg agaattgcat agagaacatg gtggttctca aagaaggtat agagaaaaca 1080gagcgtttca acatagtctc aaaggaccaa ggagtgccag tcgtcgcctt ctctctcaag 1140gaccatagtt tccacaacga gttcgagatc tctgagatgc tacgtcgttt tggctggatc 1200gtcccagctt acactatgcc tgccgatgta cagcacatca cggttctgcg tgttgtcatc 1260agggaagatt tctcaagaac actcgcggag agacttgttg ctgatatttc gaaggtgctt 1320catgagctag ataccttgcc ttccaagata tctaagaaga tgggaataga agggatcgcg 1380gaaaatgtaa aggagaagaa gatggagaag gagattctga tggaagttat tgttggatgg 1440aggaagtttg tgaaggagag gaagaagatg aatggtgtgt gctaa 1485921476DNANicotiana tabacum 92atggttctct cgaaaacctc ctctgagtcg gacgtttcgg tacactccac ttttgcctct 60cgctatgttc gaacttccct tccaaggttt gagatggcgg agaattcgat accaaaagag 120gcggcatttc aaataattaa cgacgagttg atgcttgacg ggaatccaag gctgaacttg 180gcttcatttg tgacaacatg gatggagcca gagtgtgata agcttatgat ggactccatt 240aacaagaact atgttgacat ggatgaatac cctgttacca ctgagcttca gaatcgctgc 300gtgaacatga tagcacgttt attcaacgcg ccactagaag agaaggagac agcagttgga 360gtgggtacag ttggttcatc ggaggccata atgctagcgg ggcttgcatt caagagaaat 420tggcaaaaca aacgcaaagc tgagggcaaa ccttacaata agcccaacat tgtcactggc 480gccaatgttc aggtgtgctg ggagaaattt gccaactatt ttgaagtgga attgaaagaa 540gtaaagctaa gggaagggta ctatgtgatg gacccagtcc aggctgtgga gatggttgat 600gagaacacca tttgtgttgc tgcaatcttg ggttcaaccc ttaatggaga atttgaagat 660gtcaagctct tgaatgatct tttgattgaa aagaacaagc aaactggatg gaacacacca 720attcatgtgg atgcagcaag tggtggattc attgcaccat tcctgtaccc agagctggag 780tgggacttta ggcttccctt agtgaagagc ataaatgtga gtgggcacaa atatgggctt 840gtctatgctg gtattggttg ggttatttgg aggaccaaac aagatttgcc tgaagaactc 900attttccaca tcaactatct tggagctgat cagcccacct ttactctcaa tttctccaaa 960ggttcaagtc aagtcattgc tcaatattat cagctaatcc gtttgggcta tgaggggtac 1020cgaaatgtaa tggagaactg tcgcgaaaat gccattgtgc taagagaagg actcgaaaaa 1080acaggacgtt ttaacatagt ctccaaagat gaaggtgtcc ctttggtggc cttttccctc 1140aaggacaata gccgtcacaa tgagttcgag gtgtccgaga cgctccgtag gttcgggtgg 1200atcgtcccgg cctacacgat gcccgctgac gcccaacacg tcacggtgct tcgtgtggtg 1260atccgggagg acttctcgcg aaccctagca gagcgtctcg tcctcgacat tgtcaaggtc 1320ctccacgagc tggacacact tccagctagg ctgagcgcca aattagagga ggtgaagctg 1380gtcaagaatg gaaagaaatt tgaacttgaa gttcaaaggg aagttaccaa ttattggaag 1440aagtttgttt tagctaggaa agcacctgtt tgctag 1476931503DNAOryza sativa Japonica 93atggttctga cgcacgtcga ggcggtggag gagggcagcg aggcggcggc cgccgtgttc 60gcgtcgaggt acgtgcagga cccggtgccg aggtacgagc tcggcgagag gtcgatatcc 120aaggacgccg cgtaccagat cgtccacgac gagctcctcc tggacagcag cccgcgcctg 180aacctggcgt ccttcgtcac cacctggatg gagcccgagt gcgacaggct catcctcgag 240gccatcaaca agaactacgc cgacatggac gagtaccccg tcaccaccga gctccagaac 300cggtgcgtga acatcatagc gaggctgttc aatgcgccgg tgggcgacgg cgagaaggcg 360gtcggggtgg gcacggtggg gtcgtcggag gccataatgc tggccgggct ggcgttcaag 420cggcggtggc agaaccggcg gaaggcggcg gggaagcccc acgacaagcc caacatcgtg 480acgggggcca acgtgcaggt gtgctgggag aagttcgcgc gctacttcga ggtggagctc 540aaggaggtga agctgaccga aggctgctac gtgatggacc ccgtcaaggc cgtggacatg 600gtcgacgaga acaccatctg cgtcgccgcc atcctcggct ccaccctcac cggcgagttc 660gaggacgtca ggcgcctcaa cgacctcctc gccgccaaga acaagcggac gggttgggac 720acgccgatcc acgtcgacgc ggcgagcggc gggttcatcg cgccgttcat ctacccggag 780ctggagtggg acttccggct gccgctggtg aagagcatca acgtcagcgg ccacaagtac 840gggctcgtct acgccggcgt cgggtgggtc atctggcgca acaaggagga cctccccgag 900gagctcatct tccacatcaa ctacctcggc gccgaccagc caaccttcac gctcaacttc 960tccaaagggt ccagtcagat tattgcgcaa tattaccagt ttcttcgact cggatttgag 1020gggtacaaga gcgtgatgaa gaactgcatg gagagcgcga ggacgctccg ggagggcctg 1080gagaagacgg ggcggttcac catcatctcc aaggaggagg gcgtgccgct ggtggccttc 1140acgttcaagg acggcgccgg cgcgcaggcc ttcaggctgt cgtcgggcct gcgccggtac 1200gggtggatcg tgccggcgta cacgatgccg gcggcgctgg agcacatgac ggtcgtccgc 1260gtcgtcgtcc gggaagactt cggccggccg ctcgccgagc ggttcctgtc ccacgtcagg 1320atggccctgg acgagatgga cctcgccgcc agggcccccg tgcccagggt gcagctcacc 1380atcgagctcg gccccgcccg gaccgccggc gaggaggcct cgatcagggt ggtcaagagc 1440gaggccgtgc ccgtgcgcaa gagcgtcccg ctcgtcgccg gcaaaaccaa gggcgtttgc 1500tag 1503941503DNAOryza sativa Japonica 94atggttctga cgcacgtcga ggcggtggag gagggcagcg aggcggcggc cgccgtgttc 60gcgtcgaggt acgtgcagga cccggtgccg aggtacgagc tcggcgagag gtcgatatcc 120aaggacgccg cgtaccagat cgtccacgac gagctcctcc tggacagcag cccgcgcctg 180aacctggcgt ccttcgtcac cacctggatg gagcccgagt gcgacaggct catcctcgag 240gccatcaaca agaactacgc cgacatggac gagtaccccg tcaccaccga gctccagaac 300cggtgcgtga acatcatagc gaggctgttc aatgcgccgg tgggcgacgg cgagaaggcg 360gtcggggtgg gcacggtggg gtcgtcggag gccataatgc tggccgggct ggcgttcaag 420cggcggtggc agaaccggcg gaaggcggcg gggaagcccc acgacaagcc caacatcgtg 480acgggggcca acgtgcaggt gtgctgggag aagttcgcgc gctacttcga ggtggagctc 540aaggaggtga agctgaccga aggctgctac gtgatggacc ccgtcaaggc cgtggacatg 600gtcgacgaga acaccatctg cgtcgccgcc atcctcggct ccaccctcac cggcgagttc 660gaggacgtca ggcgcctcaa cgacctcctc gccgccaaga acaagcggac gggttgggac 720acgccgatcc acgtcgacgc ggcgagcggc gggttcatcg cgccgttcat ctacccggag 780ctggagtggg acttccggct gccgctggtg aagagcatca acgtcagcgg ccacaagtac 840gggctcgtct acgccggcgt cgggtgggtc atctggcgca acaaggagga cctccccgag 900gagctcatct tccacatcaa ctacctcggc gccgaccagc caaccttcac gctcaacttc 960tccaaagggt ccagtcagat tattgcgcaa tattaccagt ttcttcgact cggatttgag 1020gggtacaaga gcgtgatgaa gaactgcatg gagagcgcga ggacgctccg ggagggcctg 1080gagaagacgg ggcggttcac catcatctcc aaggaggagg gcgtgccgct ggtggccttc 1140acgttcaagg acggcgccgg cgcgcaggcc ttcaggctgt cgtcgggcct gcgccggtac 1200gggtggatcg tgccggcgta cacgatgccg gcggcgctgg agcacatgac ggtcgtccgc 1260gtcgtcgtcc gggaagactt cggccggccg ctcgccgagc ggttcctgtc ccacgtcagg 1320atggccctgg acgagatgga cctcgccgcc agggcccccg tgcccagggt gcagctcacc 1380atcgagctcg gccccgcccg gaccgccggc gaggaggcct cgatcagggt ggtcaagagc 1440gaggccgtgc ccgtgcgcaa gagcgtcccg ctcgtcgccg gcaaaaccaa gggcgtttgc 1500tag 1503951491DNANicotiana tabacum 95atggttctgt ccaagacagc gtcggaaagt gacgtctcca tccactccac tttcgcttcc 60cgatatgttc gaacttctct tcccaggttt aagatgccag agaattctat accaaaagaa 120gcagcatatc aaatcataaa tgatgagctt atgttagatg gaaatccaag gctaaattta 180gcatcttttg tgacaacatg gatggaacca gagtgtaata agttaatgat ggattccatt 240aacaagaact acgttgacat gggtgaatac cctgtaacca ctgagcttca gaatcgatgt 300gtaaatatga tagctcattt gtttaacgca ccacttggag atggagagac tgcagttgga 360gttggaactg ttggatcctc tgaggctatt atgcttgctg gattagcctt caagagaaaa 420tggcaaaata aaatgaaagc ccaagggaag ccctgcgaca agcccaatat tgtcactggt 480gccaatgtcc aggtgtgttg ggagaaattt gcaaggtatt ttgaagtgga gttgaaagaa 540gtaaaattga gtgatggata ctatgtgatg gaccctgaga aagctgtgga aatggtggat 600gagaatacaa tttgtgtagc tgctatcttg ggttccactc tcaatggtga atttgaagat 660gttaagcgct tgaatgacct cttgattgag aagaacaaag aaaccgggtg ggacactcca 720attcatgtgg atgcagcaag tggtggattc attgcaccat tcctttatcc tgagcttgaa 780tgggatttta gattaccatt ggtgaagagt attaatgtga gtggtcacaa atatggtctt 840gtctatgctg gtattggttg ggccatttgg aggaataagg aagacttgcc tgatgaactt 900attttccaca ttaattatct tggtgctgat caacctactt tcactctcaa cttctctaaa 960ggttctagcc aagtaattgc tcaatattac caacttattc gcttgggttt tgagggttac 1020aagaatgtta tggagaattg tcaagaaaat gcaagggtac taagagaagg acttgaaaaa 1080agtggaagat tcaatataat ctccaaagaa attggagttc cattagtagc tttctctctt 1140aaagacaaca gtcaacacaa tgagttcgaa atttctgaaa ctcttagaag atttggatgg 1200attattcctg catatactat gccaccaaat gctcaacatg tcacagttct cagagttgtt 1260attagagaag atttctcccg tacactcgcg gagcgactgg tgatagacat tgaaaaagtc 1320ctccacgagc tagacacact tccggcgagg gtcaacgcta agctcgccgt ggccgaggcg 1380aatggcagcg gcgtgcataa gaaaacagat agagaagtgc agctggagat tactgctgca 1440tggaagaaat ttgttgctga taagaagaag aagactaatg gagtttgtta a 1491961485DNAArabidopsis thaliana 96atggtactcg caaccaactc tgactccgac gagcatttgc attccacttt tgcttctaga 60tatgtccgtg ctgttgttcc caggttcaag atgcctgacc attgcatgcc caaagatgct 120gcttatcaag tgatcaatga tgagttgatg cttgatggta atcccaggct taacctagcc 180tcctttgtca ccacttggat ggaacctgag tgtgacaaac tcatcatgga ttctgtcaat 240aagaactatg ttgatatgga tgaatatcct gtcaccactg agctccagaa ccggtgtgta 300aatatgatag caaacttgtt ccatgctccc gttggagaag acgaggctgc tattgggtgt 360ggaactgttg gttcatctga ggctataatg cttgctggtt tggctttcaa aaggaaatgg 420caacatagga gaaaagctca gggtctacct attgataagc ctaacattgt cactggagcc 480aatgttcagg tgtgctggga gaagtttgca aggtactttg aggtagagct caaagaggtg 540aaactaagtg aagactacta tgttatggat ccagctaaag ctgtagagat ggtggatgag 600aataccatct gtgttgcagc aattctagga tccacactta ctggagagtt tgaggacgtt 660aagcaattga acgatctctt agctgagaaa aacgcagaga caggatggga aactcctatt 720catgttgatg cagccagtgg aggattcatt gctcctttcc tctaccctga tcttgaatgg 780gactttaggc ttccatgggt gaagagtatt aacgtcagtg gtcacaagta tggacttgtg 840tatgcaggag ttggttgggt tgtctggaga acaaaagatg atttgccaga ggaacttgtc 900ttccacatca actacttggg agctgatcaa cccactttca ctctcaactt ctcaaaaggg 960tcgagccaaa tcattgctca gtactatcag tttatccgac taggctttga gggatacaag 1020aacataatgg aaaactgcat ggataacgca aggaggctaa gagaaggaat agagatgaca 1080gggaagttca acattgtgtc caaagatatt ggcgtgccac tagtggcatt ctctctcaaa 1140gacagtagca agcacacggt gtttgagatc gcagagtctt tgagaaaatt cgggtggatc 1200ataccggctt acactatgcc tgcagatgca cagcacattg ctgtgctcag agttgtgata 1260agagaagact ttagccgagg ccttgcagat agactcatca cacatatcat tcaggtgctg 1320aaagagattg aagggcttcc tagcaggatt gcacatcttg ctgcggctgc agcggttagt 1380ggtgatgatg aagaagttaa agtgaagact gccaagatgt ccttggagga tatcactaag 1440tattggaaac gccttgtgga acacaagaga aatattgtct gctaa 148597484PRTOryza sativa 97Met Ala Leu Ser Thr Ala Gln Thr Gly Glu Ser Met His Ser Ser Thr1 5 10 15Phe Ala Ser Arg Tyr Val Arg Thr Ala Leu Pro Arg Phe Arg Met Pro 20 25 30Glu Lys Ser Ile Pro Lys Asp Ala Ala Tyr Gln Ile Ile Asn Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Ala Ala Ile Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Ile Gly 100 105 110Asp Asp Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Arg Met 130 135 140Lys Ala Glu Gly Lys Pro His Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Lys Leu Thr Gln Gly Tyr Tyr Val Met Asn Pro Glu 180 185 190Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Met Leu Asn 210 215 220Asp Leu Leu Thr Ala Lys Asn Ala Glu Thr Gly Trp Asn Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly

Gly Phe Ile Ala Pro Phe Ile Tyr Pro 245 250 255Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp Val Ile 275 280 285Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Asn Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asp Ile Met Gln Asn Cys Arg Asp Asn Ala Thr Val 340 345 350Leu Arg Glu Gly Ile Glu Lys Thr Gly His Phe Asp Val Val Ser Lys 355 360 365Asp Ser Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser Arg 370 375 380Tyr Thr Val Phe Glu Val Ala Glu Ser Leu Arg Arg Phe Gly Trp Ile385 390 395 400Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Glu His Val Ala Val Met 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Gly Leu Ala Glu Arg Leu 420 425 430Ile Thr Asp Leu Thr Lys Thr Val Ala Asp Met Asp Ala His Ala Val 435 440 445Lys Lys Ala Ala Ala Glu Pro Ala Lys Lys Thr Val Arg Glu Ile Glu 450 455 460Lys Glu Val Thr Thr Tyr Trp Arg Ser Phe Val Ala Arg Lys Lys Ser465 470 475 480Ser Leu Val Cys98502PRTArabidopsis thaliana 98Met Val Leu Ser His Ala Val Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ser Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Ala Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Val Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Gln Gln Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335His Glu Gly Tyr Arg Asn Val Met Glu Asn Cys Arg Glu Asn Met Ile 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Glu Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Cys His Thr Glu Phe Glu Ile Ser Asp Met Leu Arg Arg Tyr Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Met Arg Glu Leu Asp Glu Leu Pro 435 440 445Ser Arg Val Ile His Lys Ile Ser Leu Gly Gln Glu Lys Ser Glu Ser 450 455 460Asn Ser Asp Asn Leu Met Val Thr Val Lys Lys Ser Asp Ile Asp Lys465 470 475 480Gln Arg Asp Ile Ile Thr Gly Trp Lys Lys Phe Val Ala Asp Arg Lys 485 490 495Lys Thr Ser Gly Ile Cys 50099497PRTOryza sativa 99Met Val Leu Ser His Ala Ser Ser Gly Arg Asp Asp Ala Val Arg Cys1 5 10 15Thr Phe Ala Thr Arg Tyr Ala Cys Glu Thr Leu Pro Arg Phe Arg Met 20 25 30Pro Glu Gln Ser Ile Pro Arg Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Val65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Ile 100 105 110Lys Glu Asp Glu Thr Ala Ile Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Glu Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asn Leu Leu Thr Glu Lys Asn Lys Glu Thr Gly Trp Asp Val Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly Val Gly Trp Val 275 280 285Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Ile Met Gln Asn Cys Met Glu Asn Thr Ala 340 345 350Ile Leu Arg Glu Gly Ile Glu Ala Thr Gly Arg Phe Glu Ile Leu Ser 355 360 365Lys Glu Ala Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Gly 370 375 380Arg Tyr Thr Val Phe Asp Ile Ser Glu His Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asn Ala Glu His Val Ala Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Ser Leu Ala Glu Arg 420 425 430Leu Val Ser Asp Ile Val Lys Ile Leu His Glu Leu Asp Ala His Ser 435 440 445Ala Gln Val Leu Lys Ile Ser Ser Ala Ile Ala Lys Gln Gln Ser Gly 450 455 460Asp Asp Gly Val Val Thr Lys Lys Ser Val Leu Glu Thr Glu Arg Glu465 470 475 480Ile Phe Ala Tyr Trp Arg Asp Gln Val Lys Lys Lys Gln Thr Gly Ile 485 490 495Cys100493PRTBrassica juncea 100Met Val Leu Ser Lys Thr Ala Ser Gly Thr Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Asn Ser Leu Pro Arg Phe Glu Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Gln Gly Leu Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Gly225 230 235 240Asn His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Ser Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asn Glu Phe Glu Val Ala Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Val Pro Ala Asp Ala Glu His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Arg Ala Lys Met Ala Asn Gly Lys Ala Lys Val Val Lys 450 455 460Gln Thr Glu Glu Glu Thr Thr Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Phe Val Glu Thr Lys Lys Thr Asn Gln Asn Lys Ile Cys 485 490101493PRTBrassica juncea 101Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Glu Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Gln Gly Leu Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Gly225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Ser Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asp Glu Phe Glu Val Ala Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Gln Ala Lys Met Ala Asn Gly Asn Ala Asn Gly Val Lys 450 455 460Lys Thr Glu Glu Glu Thr Thr Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Phe Val Glu Ala Lys Lys Ser Asn Lys Asn Arg Ile Cys 485 490102494PRTBrassica juncea 102Met Val Leu Ser Arg Ala Ala Thr Glu Ser Gly Glu Asn Val Cys Ser1 5 10 15Thr Phe Gly Ser Arg Tyr Val Arg Thr Ala Leu Pro Lys His Lys Ile 20 25 30Gly Glu Ser Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Lys Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Thr Glu Thr Ala Met Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Asn Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Asp Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly Trp Asn Thr Pro225 230 235

240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Gln Gln Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Arg Glu Asn Met Val 340 345 350Val Leu Arg Glu Gly Ile Glu Lys Thr Glu Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Val Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp His Ser 370 375 380Phe His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Ile Val Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ser Lys Ile Ser Lys Lys Met Gly Ala Glu Asp Phe Gly Asn Val Lys 450 455 460Gly Lys Lys Val Asp Arg Asp Val Leu Met Glu Val Ile Val Gly Trp465 470 475 480Arg Lys Phe Val Lys Asp Arg Lys Lys Met Asn Gly Val Cys 485 490103494PRTArabidopsis thaliana 103Met Val Leu Ala Thr Asn Ser Asp Ser Asp Glu His Leu His Ser Thr1 5 10 15Phe Ala Ser Arg Tyr Val Arg Ala Val Val Pro Arg Phe Lys Met Pro 20 25 30Asp His Cys Met Pro Lys Asp Ala Ala Tyr Gln Val Ile Asn Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Val Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Met Ile Ala Asn Leu Phe His Ala Pro Val Gly 100 105 110Glu Asp Glu Ala Ala Ile Gly Cys Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln His Arg Arg 130 135 140Lys Ala Gln Gly Leu Pro Ile Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Lys Leu Ser Glu Asp Tyr Tyr Val Met Asp Pro Ala 180 185 190Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Gln Leu Asn 210 215 220Asp Leu Leu Ala Glu Lys Asn Ala Glu Thr Gly Trp Glu Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr Pro 245 250 255Asp Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp Val Val 275 280 285Trp Arg Thr Lys Asp Asp Leu Pro Glu Glu Leu Val Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Met Asp Asn Ala Arg Arg 340 345 350Leu Arg Glu Gly Ile Glu Met Thr Gly Lys Phe Asn Ile Val Ser Lys 355 360 365Asp Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser Lys 370 375 380His Thr Val Phe Glu Ile Ala Glu Ser Leu Arg Lys Phe Gly Trp Ile385 390 395 400Ile Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Ala Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Gly Leu Ala Asp Arg Leu 420 425 430Ile Thr His Ile Ile Gln Val Leu Lys Glu Ile Glu Gly Leu Pro Ser 435 440 445Arg Ile Ala His Leu Ala Ala Ala Ala Ala Val Ser Gly Asp Asp Glu 450 455 460Glu Val Lys Val Lys Thr Ala Lys Met Ser Leu Glu Asp Ile Thr Lys465 470 475 480Tyr Trp Lys Arg Leu Val Glu His Lys Arg Asn Ile Val Cys 485 490104493PRTArabidopsis thaliana 104Met Val Leu Ser Lys Thr Val Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Asn Ser Leu Pro Arg Phe Glu Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Gln Gly Leu Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Asn Leu Arg Glu Asp Tyr Tyr Val Met Asp Pro 180 185 190Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Gln Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Thr Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Gln Gly Leu Glu Lys Thr Gly Arg Phe Lys Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asn Glu Phe Glu Val Ala His Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val His Ala Lys Met Ala Asn Gly Lys Val Asn Gly Val Lys 450 455 460Lys Thr Pro Glu Glu Thr Gln Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Leu Leu Glu Thr Lys Lys Thr Asn Lys Asn Thr Ile Cys 485 490105500PRTArabidopsis thaliana 105Met Val Leu Ser Lys Thr Ala Ser Lys Ser Asp Asp Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Asn Ser Ile Ser Arg Phe Glu Ile 20 25 30Pro Lys Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Lys Phe Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Glu Ser Ile65 70 75 80Asn Lys Asn Asn Val Glu Met Asp Gln Tyr Pro Val Thr Thr Asp Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Ala Ala Ile Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Val Met Leu Ala Gly Leu Ala Phe Lys Arg Gln Trp Gln Asn Lys 130 135 140Arg Lys Ala Leu Gly Leu Pro Tyr Asp Arg Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Ile Gln Val Cys Leu Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Asp Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Val Ala 195 200 205Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Lys Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Asp Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Thr Lys Thr Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Arg Asn Val Met Asp Asn Cys Arg Glu Asn Met Met 340 345 350Val Leu Arg Gln Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Glu Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Arg His Asn Glu Phe Glu Val Ala Glu Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ala Asp Phe Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val His Ala Lys Met Ala Ser Gly Lys Val Asn Gly Val Lys 450 455 460Lys Thr Pro Glu Glu Thr Gln Arg Glu Val Thr Ala Tyr Trp Lys Lys465 470 475 480Phe Val Asp Thr Lys Thr Asp Lys Asn Gly Val Pro Leu Val Ala Ser 485 490 495Ile Thr Asn Gln 500106491PRTNicotiana tabacum 106Met Val Leu Ser Lys Thr Ser Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Glu Met 20 25 30Ala Glu Asn Ser Ile Pro Lys Glu Ala Ala Phe Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala Arg Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Lys Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Asn Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Tyr Asn Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Asn Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Val Gln Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Gln Thr Gly Trp Asn Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Ile Trp Arg Thr Lys Gln Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Tyr Glu Gly Tyr Arg Asn Val Met Glu Asn Cys Arg Glu Asn Ala Ile 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Arg His Asn Glu Phe Glu Val Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Ile Val Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Leu Ser Ala Lys Leu Glu Glu Val Lys Leu Val Lys Asn Gly 450 455 460Lys Lys Phe Glu Leu Glu Val Gln Arg Glu Val Thr Asn Tyr Trp Lys465 470 475 480Lys Phe Val Leu Ala Arg Lys Ala Pro Val Cys 485 490107496PRTNicotiana tabacum 107Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Gly Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215

220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Ile Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Ala Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 495108450PRTGlycine max 108Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr Thr Trp Met Glu1 5 10 15Pro Glu Cys Asp Lys Leu Ile Met Ala Ala Ile Asn Lys Asn Tyr Val 20 25 30Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln Asn Arg Cys Val 35 40 45Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu Glu Glu Thr Glu Ala 50 55 60Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala Ile Met Leu Ala65 70 75 80Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Arg Arg Lys Gln Glu Gly 85 90 95Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly Ala Asn Val Gln Val 100 105 110Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu Leu Lys Glu Val 115 120 125Lys Leu Arg Asp Asp Tyr Tyr Val Met Asp Pro Glu Lys Ala Val Glu 130 135 140Leu Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile Leu Gly Ser Thr145 150 155 160Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu Asn Asp Leu Leu Ile 165 170 175Glu Lys Asn Lys Ile Thr Gly Trp Asp Thr Pro Ile His Val Asp Ala 180 185 190Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr Pro Glu Leu Glu Trp 195 200 205Asp Phe Arg Leu Gln Leu Val Lys Ser Ile Asn Val Ser Gly His Lys 210 215 220Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val Ile Trp Arg Ser Lys225 230 235 240Gln Asp Leu Pro Glu Glu Leu Ile Phe His Ile Asn Tyr Leu Gly Ala 245 250 255Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly Ser Ser Gln Val 260 265 270Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe Glu Gly Tyr Arg 275 280 285Asn Val Met Glu Asn Cys Arg Asp Asn Met Leu Val Leu Lys Glu Gly 290 295 300Leu Glu Lys Thr Gly Arg Phe Ser Ile Val Ser Lys Asp Asn Gly Val305 310 315 320Pro Leu Val Ala Phe Thr Leu Lys Asp His Thr His Phe Asp Glu Phe 325 330 335Gln Ile Ser Asp Phe Leu Arg Arg Phe Gly Trp Ile Val Pro Ala Tyr 340 345 350Thr Met Pro Pro Asp Ala Gln His Val Thr Val Leu Arg Val Val Ile 355 360 365Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg Leu Val Ser Asp Val 370 375 380Glu Lys Val Leu His Glu Leu Asp Ser Leu Pro Ala Arg Val Ile Ser385 390 395 400Ser Thr Thr Val Thr Leu Ser Ala Glu Glu Asn Gly Lys Val Val Val 405 410 415Ala Lys Lys Asn Pro Met Glu Thr Gln Arg Glu Ile Thr Ala Ile Trp 420 425 430Lys Lys Phe Val Leu Glu Arg Lys Lys Asn Asn Asp Lys Met Asn Gly 435 440 445Val Cys 450109496PRTNicotiana tabacum 109Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Thr Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Ile Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 495110494PRTArabidopsis thaliana 110Met Val Leu Ala Thr Asn Ser Asp Ser Asp Glu His Leu His Ser Thr1 5 10 15Phe Ala Ser Arg Tyr Val Arg Ala Val Val Pro Arg Phe Lys Met Pro 20 25 30Asp His Cys Met Pro Lys Asp Ala Ala Tyr Gln Val Ile Asn Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Val Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Met Ile Ala Asn Phe Phe His Ala Pro Val Gly 100 105 110Glu Asp Glu Ala Ala Ile Gly Cys Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln His Arg Arg 130 135 140Lys Ala Gln Gly Leu Pro Ile Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Lys Leu Ser Glu Asp Tyr Tyr Val Met Asp Pro Ala 180 185 190Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Gln Leu Asn 210 215 220Asp Leu Leu Ala Glu Lys Asn Ala Glu Thr Gly Trp Glu Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr Pro 245 250 255Asp Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp Val Val 275 280 285Trp Arg Thr Lys Asp Asp Leu Pro Glu Glu Leu Val Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Met Asp Asn Ala Arg Arg 340 345 350Leu Arg Glu Gly Ile Glu Met Thr Gly Lys Phe Asn Ile Val Ser Lys 355 360 365Asp Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser Lys 370 375 380His Thr Val Phe Glu Ile Ala Glu Ser Leu Arg Lys Phe Gly Trp Ile385 390 395 400Ile Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Ala Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Gly Leu Ala Asp Arg Leu 420 425 430Ile Thr His Ile Ile Gln Val Leu Lys Glu Ile Glu Gly Leu Pro Ser 435 440 445Arg Ile Ala His Leu Ala Ala Ala Ala Ala Val Ser Gly Asp Asp Glu 450 455 460Glu Val Lys Val Lys Thr Ala Lys Met Ser Leu Glu Asp Ile Thr Lys465 470 475 480Tyr Trp Lys Arg Leu Val Glu His Lys Arg Asn Ile Val Cys 485 490111492PRTOryza sativa 111Met Val Leu Ser Lys Ala Val Ser Glu Ser Asp Met Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Ala Ser Leu Pro Arg Tyr Arg Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ala Ala Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe His Ala Pro Leu 100 105 110Gly Glu Asp Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Phe Asp Lys Pro Asn Ile Ile Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Arg Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Asp Met Val Asn Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Asp Lys Lys Asn Lys Glu Thr Gly Trp Glu Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Trp Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Cys 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg His Gly 325 330 335Phe Glu Gly Tyr Arg Asn Ile Met Glu Asn Cys His Glu Asn Ala Met 340 345 350Val Leu Lys Glu Gly Leu Val Lys Thr Gly Arg Phe Asp Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Arg Ser 370 375 380Arg His Asp Glu Phe Glu Ile Ser Asp Met Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Glu Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Ile Glu Lys Val Met Tyr Gln Leu Asp Ala Leu Pro 435 440 445Ser Arg Leu Met Pro Pro Val Pro Pro Ala Pro Leu Leu Val Val Ala 450 455 460Lys Lys Ser Glu Leu Glu Thr Gln Arg Ser Val Thr Glu Ala Trp Lys465 470 475 480Lys Phe Val Leu Ala Lys Arg Thr Asn Gly Val Cys 485 490112419PRTArabidopsis thaliana 112Met Asp Ser Ile Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val1 5 10 15Thr Thr Glu Leu Gln Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe 20 25 30Asn Ala Pro Leu Glu Glu Ser Glu Thr Ala Val Gly Val Gly Thr Val 35 40 45Gly Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys 50 55 60Trp Gln Asn Lys Arg Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn65 70 75 80Ile Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg 85 90 95Tyr Phe Glu Val Glu Leu Lys Glu Val Asn Leu Ser Glu Gly Tyr Tyr 100 105 110Val Met Asp Pro Asp Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile 115 120 125Cys Val Ala Ala Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp 130 135 140Val Lys Arg Leu Asn Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly145 150 155 160Trp Asn Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala 165 170 175Pro Phe Ile Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val 180 185 190Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly 195 200 205Ile Gly Trp Val Val Trp Arg Ala Ala Glu Asp Leu Pro Glu Glu Leu 210 215 220Ile Phe His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu225 230 235 240Asn Phe Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu 245

250 255Ile Arg Leu Gly Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Ile 260 265 270Glu Asn Met Val Val Leu Lys Glu Gly Ile Glu Lys Thr Glu Arg Phe 275 280 285Asn Ile Val Ser Lys Asp Gln Gly Val Pro Val Val Ala Phe Ser Leu 290 295 300Lys Asp His Ser Phe His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg305 310 315 320Arg Phe Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln 325 330 335His Ile Thr Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr 340 345 350Leu Ala Glu Arg Leu Val Ala Asp Ile Ser Lys Val Leu His Glu Leu 355 360 365Asp Thr Leu Pro Ser Lys Ile Ser Lys Lys Met Gly Ile Glu Gly Ile 370 375 380Ala Lys Asn Val Lys Glu Lys Lys Met Glu Lys Glu Ile Leu Met Glu385 390 395 400Val Ile Val Gly Trp Arg Lys Phe Val Lys Glu Arg Lys Lys Met Asn 405 410 415Gly Val Cys113501PRTOryza sativa 113Met Val Val Ser Val Ala Ala Thr Asp Ser Asp Thr Ala Gln Pro Val1 5 10 15Gln Tyr Ser Thr Phe Phe Ala Ser Arg Tyr Val Arg Asp Pro Leu Pro 20 25 30Arg Phe Arg Met Pro Glu Gln Ser Ile Pro Arg Glu Ala Ala Tyr Gln 35 40 45Ile Ile Asn Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu 50 55 60Ala Ser Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile65 70 75 80Met Asp Ser Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val 85 90 95Thr Thr Glu Leu Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe 100 105 110Asn Ala Pro Ile Lys Glu Asp Glu Thr Ala Ile Gly Val Gly Thr Val 115 120 125Gly Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys 130 135 140Trp Gln Asn Lys Arg Lys Glu Gln Gly Lys Pro Cys Asp Lys Pro Asn145 150 155 160Ile Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg 165 170 175Tyr Phe Glu Val Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr 180 185 190Val Met Asp Pro Val Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile 195 200 205Cys Val Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp 210 215 220Val Lys Leu Leu Asn Asn Leu Leu Thr Glu Lys Asn Lys Glu Thr Gly225 230 235 240Trp Asp Val Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala 245 250 255Pro Phe Leu Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val 260 265 270Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly 275 280 285Val Gly Trp Val Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu 290 295 300Ile Phe His Ile Asn Tyr Leu Gly Thr Asp Gln Pro Thr Phe Thr Leu305 310 315 320Asn Phe Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu 325 330 335Ile Arg Leu Gly Phe Glu Gly Tyr Lys Asn Ile Met Gln Asn Cys Met 340 345 350Glu Asn Thr Ala Ile Leu Arg Glu Gly Ile Glu Ala Thr Gly Arg Phe 355 360 365Glu Ile Leu Ser Lys Glu Ala Gly Val Pro Leu Val Ala Phe Ser Leu 370 375 380Lys Asp Ser Gly Arg Tyr Thr Val Phe Asp Ile Ser Glu His Leu Arg385 390 395 400Arg Phe Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asn Ala Glu 405 410 415His Val Ala Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Ser 420 425 430Leu Ala Glu Arg Leu Val Ser Asp Ile Val Lys Ile Leu His Glu Leu 435 440 445Asp Ala His Ser Ala Gln Val Leu Lys Ile Ser Ser Ala Ile Ala Lys 450 455 460Gln Gln Ser Gly Asp Asp Gly Val Val Thr Lys Lys Ser Val Leu Glu465 470 475 480Thr Glu Arg Glu Ile Phe Ala Tyr Trp Arg Asp Gln Val Lys Lys Lys 485 490 495Gln Thr Gly Ile Cys 500114494PRTArabidopsis thaliana 114Met Val Leu Thr Lys Thr Ala Thr Asn Asp Glu Ser Val Cys Thr Met1 5 10 15Phe Gly Ser Arg Tyr Val Arg Thr Thr Leu Pro Lys Tyr Glu Ile Gly 20 25 30Glu Asn Ser Ile Pro Lys Asp Ala Ala Tyr Gln Ile Ile Lys Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Ile Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala Pro Leu Glu 100 105 110Glu Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys Arg 130 135 140Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Asn Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro Asp 180 185 190Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu Asn 210 215 220Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly Trp Asn Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr Pro 245 250 255Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val Val 275 280 285Trp Arg Ala Ala Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Ile Glu Asn Met Val Val 340 345 350Leu Lys Glu Gly Ile Glu Lys Thr Glu Arg Phe Asn Ile Val Ser Lys 355 360 365Asp Gln Gly Val Pro Val Val Ala Phe Ser Leu Lys Asp His Ser Phe 370 375 380His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg Arg Phe Gly Trp Ile385 390 395 400Val Pro Ala Tyr Thr Met Pro Ala Asp Val Gln His Ile Thr Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg Leu 420 425 430Val Ala Asp Ile Ser Lys Val Leu His Glu Leu Asp Thr Leu Pro Ser 435 440 445Lys Ile Ser Lys Lys Met Gly Ile Glu Gly Ile Ala Glu Asn Val Lys 450 455 460Glu Lys Lys Met Glu Lys Glu Ile Leu Met Glu Val Ile Val Gly Trp465 470 475 480Arg Lys Phe Val Lys Glu Arg Lys Lys Met Asn Gly Val Cys 485 490115496PRTNicotiana tabacum 115Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Thr Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Glu Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Ile Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Leu Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Phe His Gly Val Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Leu Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 495116494PRTArabidopsis thaliana 116Met Val Leu Thr Lys Thr Ala Thr Asn Asp Glu Ser Val Cys Thr Met1 5 10 15Phe Gly Ser Arg Tyr Val Arg Thr Thr Leu Pro Lys Tyr Glu Ile Gly 20 25 30Glu Asn Ser Ile Pro Lys Asp Ala Ala Tyr Gln Ile Ile Lys Asp Glu 35 40 45Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val Thr 50 55 60Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Asp Ser Ile Asn65 70 75 80Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu Gln 85 90 95Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala Pro Leu Glu 100 105 110Glu Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu Ala 115 120 125Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys Arg 130 135 140Lys Ala Glu Gly Lys Pro Tyr Asp Lys Pro Asn Ile Val Thr Gly Ala145 150 155 160Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val Glu 165 170 175Leu Lys Glu Val Asn Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro Asp 180 185 190Lys Ala Ala Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala Ile 195 200 205Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu Asn 210 215 220Asp Leu Leu Val Lys Lys Asn Glu Glu Thr Gly Trp Asn Thr Pro Ile225 230 235 240His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr Pro 245 250 255Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn Val 260 265 270Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val Val 275 280 285Trp Arg Ala Ala Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile Asn 290 295 300Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly305 310 315 320Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe 325 330 335Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Ile Glu Asn Met Val Val 340 345 350Leu Lys Glu Gly Ile Glu Lys Thr Glu Arg Phe Asn Ile Val Ser Lys 355 360 365Asp Gln Gly Val Pro Val Val Ala Phe Ser Leu Lys Asp His Ser Phe 370 375 380His Asn Glu Phe Glu Ile Ser Glu Met Leu Arg Arg Phe Gly Trp Ile385 390 395 400Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Gln His Ile Thr Val Leu 405 410 415Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg Leu 420 425 430Val Ala Asp Ile Ser Lys Val Leu His Glu Leu Asp Thr Leu Pro Ser 435 440 445Lys Ile Ser Lys Lys Met Gly Ile Glu Gly Ile Ala Glu Asn Val Lys 450 455 460Glu Lys Lys Met Glu Lys Glu Ile Leu Met Glu Val Ile Val Gly Trp465 470 475 480Arg Lys Phe Val Lys Glu Arg Lys Lys Met Asn Gly Val Cys 485 490117496PRTNicotiana tabacum 117Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr

Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Ile Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 495118496PRTNicotiana tabacum 118Met Val Leu Ser Lys Thr Ala Ser Glu Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asn Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Ile Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Glu Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Ala 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Arg 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Ser Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Ser 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Ile Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Ala Asn Gly Ser Gly 450 455 460Val His Lys Lys Thr Asp Arg Glu Val Gln Leu Glu Ile Thr Thr Ala465 470 475 480Trp Lys Lys Phe Val Ala Asp Lys Lys Lys Lys Thr Asn Gly Val Cys 485 490 495119502PRTArabidopsis thaliana 119Met Val Leu Ser His Ala Val Ser Glu Ser Asp Val Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ser Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Glu Glu Ala Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Arg Lys Ala Glu Gly Lys Pro Val Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Gln Gln Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala Asp 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335His Glu Gly Tyr Arg Asn Val Met Glu Asn Cys Arg Glu Asn Met Ile 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Glu Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Glu Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ser 370 375 380Cys His Thr Glu Phe Glu Ile Ser Asp Met Leu Arg Arg Tyr Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Ile Asp Ile Glu Lys Val Met Arg Glu Leu Asp Glu Leu Pro 435 440 445Ser Arg Val Ile His Lys Ile Ser Leu Gly Gln Glu Lys Ser Glu Ser 450 455 460Asn Ser Asp Asn Leu Met Val Thr Val Lys Lys Ser Asp Ile Asp Lys465 470 475 480Gln Arg Asp Ile Ile Thr Gly Trp Lys Lys Phe Val Ala Asp Arg Lys 485 490 495Lys Thr Ser Gly Ile Cys 500120500PRTPetunia x hybrida 120Met Val Leu Ser Lys Thr Val Ser Gln Ser Asp Val Ser Ile His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Lys Met 20 25 30Pro Asp Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Met Met Asp Ser Ile65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Glu Asp Gly Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Asn Lys 130 135 140Met Lys Ala Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Glu Gly Tyr Tyr Val Met Asp Pro 180 185 190Glu Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Arg Leu 210 215 220Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Ile Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Val 275 280 285Val Trp Arg Asn Lys Asp Asp Leu Pro Asp Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Tyr Glu Gly Tyr Lys Asn Val Met Glu Asn Cys Gln Glu Asn Ala Ser 340 345 350Val Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Ile Ser 355 360 365Lys Glu Ile Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Asn Arg 370 375 380Gln His Asn Glu Phe Glu Ile Ser Glu Thr Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asn Ala Gln His Ile Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Arg Asp Ile Glu Lys Val Leu His Glu Leu Asp Thr Leu Pro 435 440 445Ala Arg Val Asn Ala Lys Leu Ala Val Ala Glu Glu Gln Ala Ala Ala 450 455 460Asn Gly Ser Glu Val His Lys Lys Thr Asp Ser Glu Val Gln Leu Glu465 470 475 480Met Ile Thr Ala Trp Lys Lys Phe Val Glu Glu Lys Lys Lys Lys Thr 485 490 495Asn Arg Val Cys 500121282PRTOryza sativa 121Gly Glu Phe Glu Asp Val Lys Leu Leu Asn Asn Leu Leu Thr Glu Lys1 5 10 15Asn Lys Glu Thr Gly Trp Asp Val Pro Ile His Val Asp Ala Ala Ser 20 25 30Gly Gly Phe Ile Ala Pro Phe Leu Tyr Pro Glu Leu Glu Trp Asp Phe 35 40 45Arg Leu Pro Leu Val Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly 50 55 60Leu Val Tyr Pro Gly Val Gly Trp Val Ile Trp Arg Ser Lys Glu Asp65 70 75 80Leu Pro Glu Glu Leu Ile Phe His Ile Asn Tyr Leu Gly Thr Asp Gln 85 90 95Pro Thr Phe Thr Leu Asn Phe Ser Lys Gly Ser Ser Gln Ile Ile Ala 100 105 110Gln Tyr Tyr Gln Leu Ile Arg Leu Gly Phe Glu Gly Tyr Lys Asn Ile 115 120 125Met Gln Asn Cys Met Glu Thr Pro Ala Ile Leu Arg Glu Gly Ile Glu 130 135 140Ala Thr Gly Arg Phe Glu Ile Leu Ser Lys Glu Ala Gly Val Pro Leu145 150 155 160Val Ala Phe Ser Leu Lys Ala Ser Gly Arg Tyr Thr Val Phe Asp Ile 165 170 175Ser Glu His Leu Arg Arg Phe Gly Trp Ile Val Pro Ala Tyr Thr Met 180 185 190Pro Ala Asn Ala Glu His Val Ala Ile Leu Arg Val Val Ile Arg Glu 195 200 205Asp Phe Ser Arg Ser Leu Ala Glu Arg Leu Val Ser Asp Ile Val Lys 210 215 220Ile Leu His Glu Leu Asp Ala His Ser Ala Gln Val Leu Lys Ile Ser225 230 235 240Ser Ala Ile Ala Lys Gln Gln Ser Gly Asp Asp Gly Ala Val Thr Lys 245 250 255Lys Ser Val Leu Glu Thr Glu Arg Glu Ile Phe Ala Tyr Trp Arg Asp 260 265 270Gln Val Lys Lys Lys Gln Thr Gly Ile Cys 275 280122502PRTLycopersicon esculentum 122Met Val Leu Thr Thr Thr Ser Ile Arg Asp Ser Glu Glu Ser Leu His1 5 10 15Cys Thr Phe Ala Ser Arg Tyr Val Gln Glu Pro Leu Pro Lys Phe Lys 20 25 30Met Pro Lys Lys Ser Met Pro Lys Glu Ala Ala Tyr Gln Ile Val Asn 35 40 45Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe 50 55 60Val Ser Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Met Ser Ser65 70 75 80Ile Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu 85 90 95Leu Gln Asn Arg Cys Val Asn Met Leu Ala His Leu Phe His Ala Pro 100 105 110Val Gly Asp Asp Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser 115 120 125Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp Gln Ser 130 135 140Lys Arg Lys Ala Glu Gly Lys Pro Phe Asp Lys Pro Asn Ile Val Thr145 150 155 160Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu 165 170 175Val Glu Leu Lys Glu Val Lys Leu Lys Glu Gly Tyr Tyr Val Met Asp 180 185 190Pro Ala Lys Ala Val Glu Ile Val Asp Glu Asn Thr Ile Cys Val Ala 195 200 205Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Lys Leu 210 215 220Leu Asn Glu Leu Leu Thr Lys Lys Asn Lys Glu Thr Gly Trp Glu Thr225 230 235 240Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu 245 250 255Trp Pro Asp Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile 260 265 270Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly Trp 275 280 285Val Ile Trp Arg Ser Lys Glu Asp Leu Pro Asp Glu Leu Val Phe His 290 295 300Ile Asn Tyr Leu Gly Ser Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser305 310 315 320Lys Gly Ser Tyr Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu 325 330 335Gly Phe Glu Gly Tyr Lys Asn Val Met Lys Asn Cys Leu Ser Asn Ala 340 345 350Lys Val Leu Thr Glu Gly Ile Thr Lys Met Gly Arg Phe Asp Ile Val 355 360 365Ser Lys Asp Val Gly Val Pro Val Val Ala Phe Ser Leu Arg Asp Ser 370 375 380Ser Lys Tyr Thr Val Phe Glu Val Ser Glu His Leu Arg Arg Phe Gly385 390 395 400Trp Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Glu His Ile Ala 405 410 415Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser His Ser Leu Ala Glu 420 425 430Arg Leu Val Ser Asp Ile Glu Lys Ile Leu Ser Glu Leu Asp Thr Gln 435 440 445Pro Pro Arg Leu Pro Thr Lys Ala Val Arg Val Thr Ala Glu Glu Val 450 455 460Arg Asp Asp Lys Gly Asp Gly Leu His His Phe His Met Asp Thr Val465 470 475 480Glu Thr Gln Lys Asp Ile Ile Lys His Trp Arg Lys

Ile Ala Gly Lys 485 490 495Lys Thr Ser Gly Val Cys 500123500PRTOryza sativa 123Met Val Leu Thr His Val Glu Ala Val Glu Glu Gly Ser Glu Ala Ala1 5 10 15Ala Ala Val Phe Ala Ser Arg Tyr Val Gln Asp Pro Val Pro Arg Tyr 20 25 30Glu Leu Gly Glu Arg Ser Ile Ser Lys Asp Ala Ala Tyr Gln Ile Val 35 40 45His Asp Glu Leu Leu Leu Asp Ser Ser Pro Arg Leu Asn Leu Ala Ser 50 55 60Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Arg Leu Ile Leu Glu65 70 75 80Ala Ile Asn Lys Asn Tyr Ala Asp Met Asp Glu Tyr Pro Val Thr Thr 85 90 95Glu Leu Gln Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala 100 105 110Pro Val Gly Asp Gly Glu Lys Ala Val Gly Val Gly Thr Val Gly Ser 115 120 125Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln 130 135 140Asn Arg Arg Lys Ala Ala Gly Lys Pro His Asp Lys Pro Asn Ile Val145 150 155 160Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe 165 170 175Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Cys Tyr Val Met 180 185 190Asp Pro Val Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val 195 200 205Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Arg 210 215 220Arg Leu Asn Asp Leu Leu Ala Ala Lys Asn Lys Arg Thr Gly Trp Asp225 230 235 240Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe 245 250 255Ile Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser 260 265 270Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly 275 280 285Trp Val Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe 290 295 300His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe305 310 315 320Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Leu Arg 325 330 335Leu Gly Phe Glu Gly Tyr Lys Ser Val Met Lys Asn Cys Met Glu Ser 340 345 350Ala Arg Thr Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Thr Ile 355 360 365Ile Ser Lys Glu Glu Gly Val Pro Leu Val Ala Phe Thr Phe Lys Asp 370 375 380Gly Ala Gly Ala Gln Ala Phe Arg Leu Ser Ser Gly Leu Arg Arg Tyr385 390 395 400Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Ala Leu Glu His Met 405 410 415Thr Val Leu Arg Val Val Val Arg Glu Asp Phe Gly Arg Pro Leu Ala 420 425 430Glu Arg Phe Leu Ser His Val Arg Met Ala Leu Asp Glu Met Asp Leu 435 440 445Ala Ala Arg Ala Pro Val Pro Arg Val Gln Leu Thr Ile Glu Leu Gly 450 455 460Pro Ala Arg Thr Ala Gly Glu Glu Ala Ser Ile Arg Val Val Lys Ser465 470 475 480Glu Ala Val Pro Val Arg Lys Ser Val Pro Leu Val Ala Gly Lys Thr 485 490 495Lys Gly Val Cys 500124513PRTOryza sativa 124Met Val Leu Ser His Gly Val Ser Gly Ser Asp Glu Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg His Ala Arg 20 25 30Ser Pro Leu Ser Arg Ala Pro Leu Ala Pro Ile Asp Ser Val Ile Asp 35 40 45Trp Glu Phe Arg Met Pro Glu Gln Ser Ile Pro Lys Glu Ala Ala Tyr 50 55 60Gln Ile Ile Asn Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn65 70 75 80Leu Ala Ser Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu 85 90 95Ile Gln Ala Ser Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro 100 105 110Val Thr Thr Glu Leu Gln Asn Arg Cys Val Asn Met Ile Ala His Leu 115 120 125Phe Asn Ala Pro Leu Gly Asp Ser Glu Thr Ala Val Gly Val Gly Thr 130 135 140Val Gly Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg145 150 155 160Arg Trp Gln Asn Lys Met Lys Ala Ala Gly Lys Pro Cys Asp Lys Pro 165 170 175Asn Ile Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala 180 185 190Arg Tyr Phe Glu Val Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr 195 200 205Tyr Val Met Asp Pro Ala Lys Ala Val Asp Met Val Asp Glu Asn Thr 210 215 220Ile Cys Val Ala Ala Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu225 230 235 240Asp Val Lys Leu Leu Asn Asp Leu Leu Thr Lys Lys Asn Ala Glu Thr 245 250 255Gly Trp Asp Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile 260 265 270Ala Pro Phe Leu Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu 275 280 285Val Lys Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala 290 295 300Gly Ile Gly Trp Cys Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu305 310 315 320Leu Ile Phe His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr 325 330 335Leu Asn Phe Ser Lys Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln 340 345 350Leu Ile Arg Leu Gly Phe Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys 355 360 365Gln Glu Asn Ala Met Val Leu Lys Gln Gly Leu Glu Lys Thr Gly Arg 370 375 380Phe Asn Ile Val Ser Lys Asp Asn Gly Val Pro Leu Val Ala Phe Ser385 390 395 400Leu Lys Asp Ser Ala Arg His Asn Glu Phe Glu Ile Ser Asp Phe Leu 405 410 415Arg Arg Phe Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala 420 425 430Gln His Val Thr Val Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg 435 440 445Thr Leu Ala Glu Arg Leu Val Leu Asp Val Glu Lys Val Leu His Glu 450 455 460Leu Asp Ala Leu Pro Ala Arg Val Val Ala Asn Gly Gly Asp Ala Ala465 470 475 480Ala Ala Ser Ala Ser Glu Arg Glu Met Glu Lys Gln Arg Glu Val Ile 485 490 495Ser Leu Trp Lys Arg Ala Val Leu Ala Lys Lys Lys Thr Asn Gly Val 500 505 510Cys125500PRTOryza sativa 125Met Val Leu Thr His Val Glu Ala Val Glu Glu Gly Ser Glu Ala Ala1 5 10 15Ala Ala Val Phe Ala Ser Arg Tyr Val Gln Asp Pro Val Pro Arg Tyr 20 25 30Glu Leu Gly Glu Arg Ser Ile Ser Lys Asp Ala Ala Tyr Gln Ile Val 35 40 45His Asp Glu Leu Leu Leu Asp Ser Ser Pro Arg Leu Asn Leu Ala Ser 50 55 60Phe Val Thr Thr Trp Met Glu Pro Glu Cys Asp Arg Leu Ile Leu Glu65 70 75 80Ala Ile Asn Lys Asn Tyr Ala Asp Met Asp Glu Tyr Pro Val Thr Thr 85 90 95Glu Leu Gln Asn Arg Cys Val Asn Ile Ile Ala Arg Leu Phe Asn Ala 100 105 110Pro Val Gly Asp Gly Glu Lys Ala Val Gly Val Gly Thr Val Gly Ser 115 120 125Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln 130 135 140Asn Arg Arg Lys Ala Ala Gly Lys Pro His Asp Lys Pro Asn Ile Val145 150 155 160Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe 165 170 175Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Cys Tyr Val Met 180 185 190Asp Pro Val Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val 195 200 205Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Phe Glu Asp Val Arg 210 215 220Arg Leu Asn Asp Leu Leu Ala Ala Lys Asn Lys Arg Thr Gly Trp Asp225 230 235 240Thr Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe 245 250 255Ile Tyr Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser 260 265 270Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Val Gly 275 280 285Trp Val Ile Trp Arg Asn Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe 290 295 300His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe305 310 315 320Ser Lys Gly Ser Ser Gln Ile Ile Ala Gln Tyr Tyr Gln Phe Leu Arg 325 330 335Leu Gly Phe Glu Gly Tyr Lys Ser Val Met Lys Asn Cys Met Glu Ser 340 345 350Ala Arg Thr Leu Arg Glu Gly Leu Glu Lys Thr Gly Arg Phe Thr Ile 355 360 365Ile Ser Lys Glu Glu Gly Val Pro Leu Val Ala Phe Thr Phe Lys Asp 370 375 380Gly Ala Gly Ala Gln Ala Phe Arg Leu Ser Ser Gly Leu Arg Arg Tyr385 390 395 400Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Ala Leu Glu His Met 405 410 415Thr Val Val Arg Val Val Val Arg Glu Asp Phe Gly Arg Pro Leu Ala 420 425 430Glu Arg Phe Leu Ser His Val Arg Met Ala Leu Asp Glu Met Asp Leu 435 440 445Ala Ala Arg Ala Pro Val Pro Arg Val Gln Leu Thr Ile Glu Leu Gly 450 455 460Pro Ala Arg Thr Ala Gly Glu Glu Ala Ser Ile Arg Val Val Lys Ser465 470 475 480Glu Ala Val Pro Val Arg Lys Ser Val Pro Leu Val Ala Gly Lys Thr 485 490 495Lys Gly Val Cys 500126492PRTOryza sativa 126Met Val Leu Ser His Gly Val Ser Gly Ser Asp Glu Ser Val His Ser1 5 10 15Thr Phe Ala Ser Arg Tyr Val Arg Thr Ser Leu Pro Arg Phe Arg Met 20 25 30Pro Glu Gln Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile Ile Asn Asp 35 40 45Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala Ser Phe Val 50 55 60Thr Thr Trp Met Glu Pro Glu Cys Asp Lys Leu Ile Gln Ala Ser Val65 70 75 80Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr Thr Glu Leu 85 90 95Gln Asn Arg Cys Val Asn Met Ile Ala His Leu Phe Asn Ala Pro Leu 100 105 110Gly Asp Ser Glu Thr Ala Val Gly Val Gly Thr Val Gly Ser Ser Glu 115 120 125Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Arg Trp Gln Asn Lys 130 135 140Met Lys Ala Ala Gly Lys Pro Cys Asp Lys Pro Asn Ile Val Thr Gly145 150 155 160Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr Phe Glu Val 165 170 175Glu Leu Lys Glu Val Lys Leu Ser Asp Gly Tyr Tyr Val Met Asp Pro 180 185 190Ala Lys Ala Val Asp Met Val Asp Glu Asn Thr Ile Cys Val Ala Ala 195 200 205Ile Leu Gly Ser Thr Leu Asn Gly Glu Phe Glu Asp Val Lys Leu Leu 210 215 220Asn Asp Leu Leu Thr Lys Lys Asn Ala Glu Thr Gly Trp Asp Thr Pro225 230 235 240Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro Phe Leu Tyr 245 250 255Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys Ser Ile Asn 260 265 270Val Ser Gly His Lys Tyr Gly Leu Val Tyr Ala Gly Ile Gly Trp Cys 275 280 285Ile Trp Arg Ser Lys Glu Asp Leu Pro Glu Glu Leu Ile Phe His Ile 290 295 300Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn Phe Ser Lys305 310 315 320Gly Ser Ser Gln Val Ile Ala Gln Tyr Tyr Gln Leu Ile Arg Leu Gly 325 330 335Phe Glu Gly Tyr Lys Asn Ile Met Glu Asn Cys Gln Glu Asn Ala Met 340 345 350Val Leu Lys Gln Gly Leu Glu Lys Thr Gly Arg Phe Asn Ile Val Ser 355 360 365Lys Asp Asn Gly Val Pro Leu Val Ala Phe Ser Leu Lys Asp Ser Ala 370 375 380Arg His Asn Glu Phe Glu Ile Ser Asp Phe Leu Arg Arg Phe Gly Trp385 390 395 400Ile Val Pro Ala Tyr Thr Met Pro Pro Asp Ala Gln His Val Thr Val 405 410 415Leu Arg Val Val Ile Arg Glu Asp Phe Ser Arg Thr Leu Ala Glu Arg 420 425 430Leu Val Leu Asp Val Glu Lys Val Leu His Glu Leu Asp Ala Leu Pro 435 440 445Ala Arg Val Val Ala Asn Gly Gly Asp Ala Ala Ala Ala Ser Ala Ser 450 455 460Glu Arg Glu Met Glu Lys Gln Arg Glu Val Ile Ser Leu Trp Lys Arg465 470 475 480Ala Val Leu Ala Lys Lys Lys Thr Asn Gly Val Cys 485 490127490PRTHordeum vulgare 127Met Val Val Thr Val Ala Ala Thr Gly Pro Asp Thr Ala Glu Thr Leu1 5 10 15His Ser Thr Thr Phe Ala Ser Arg Tyr Val Arg Asp Gln Leu Pro Arg 20 25 30Tyr Arg Met Pro Glu Asn Ser Ile Pro Lys Glu Ala Ala Tyr Gln Ile 35 40 45Ile Ser Asp Glu Leu Met Leu Asp Gly Asn Pro Arg Leu Asn Leu Ala 50 55 60Ser Phe Val Thr Thr Trp Met Glu Pro Glu Cys Gly Lys Leu Ile Met65 70 75 80Asp Ser Val Asn Lys Asn Tyr Val Asp Met Asp Glu Tyr Pro Val Thr 85 90 95Thr Glu Leu Gln Asp Arg Cys Val Asn Met Ile Ala His Leu Phe Asn 100 105 110Ala Pro Ile Gly Glu Asp Glu Thr Ala Ile Gly Val Ser Thr Val Gly 115 120 125Ser Ser Glu Ala Ile Met Leu Ala Gly Leu Ala Phe Lys Arg Lys Trp 130 135 140Ala Asn Lys Met Lys Glu Gln Gly Lys Pro Cys Asp Lys Pro Asn Ile145 150 155 160Val Thr Gly Ala Asn Val Gln Val Cys Trp Glu Lys Phe Ala Arg Tyr 165 170 175Phe Glu Val Glu Leu Lys Glu Val Lys Leu Thr Glu Gly Tyr Tyr Val 180 185 190Met Asp Pro Lys Lys Ala Val Glu Met Val Asp Glu Asn Thr Ile Cys 195 200 205Val Ala Ala Ile Leu Gly Ser Thr Leu Thr Gly Glu Tyr Glu Asp Val 210 215 220Lys Leu Leu Asn Asp Leu Leu Val Glu Lys Asn Lys Glu Thr Gly Trp225 230 235 240Asn Val Pro Ile His Val Asp Ala Ala Ser Gly Gly Phe Ile Ala Pro 245 250 255Phe Leu Gln Pro Glu Leu Glu Trp Asp Phe Arg Leu Pro Leu Val Lys 260 265 270Ser Ile Asn Val Ser Gly His Lys Tyr Gly Leu Val Tyr Pro Gly Val 275 280 285Gly Trp Val Ile Trp Arg Ser Lys Asp Asp Leu Pro Glu Glu Leu Ile 290 295 300Phe His Ile Asn Tyr Leu Gly Ala Asp Gln Pro Thr Phe Thr Leu Asn305 310 315 320Phe Ser Lys Gly Gln Gln Ile Ile Ala Gln Tyr Tyr Gln Leu Ile Arg 325 330 335Leu Gly Phe Glu Gly Tyr Lys His Ile Met Glu Asn Cys Lys Leu Asn 340 345 350Ala Ala Val Leu Lys Glu Gly Ile Asp Ala Thr Gly Arg Phe Asp Val 355 360 365Leu Ser Lys Ala Asp Gly Val Pro Leu Val Ala Ile Arg Leu Lys Asp 370 375 380Ser Thr Asn Phe Ser Val Phe Asp Ile Ser Glu Asn Leu Arg Arg Phe385 390 395 400Gly Trp Ile Val Pro Ala Tyr Thr Met Pro Ala Asp Ala Glu His Val 405 410 415Ala Val Leu Arg Ile Val Ile Arg Glu Asp Phe Asn Arg Ser Leu Ala 420 425 430Gln Arg Leu Leu Ala Asp Ile Asn Lys Ile Ile

Gly Glu Leu Asp Ala 435 440 445His Ala Val His Ala Ile Lys Leu Ser Thr Ala Ala Ala Gly Gly Asp 450 455 460Gly Ala Ser Lys Ser Ala Val Asp Ala Ala Thr Glu Ala Phe Lys Asp465 470 475 480Leu Ala Gly Lys Lys Lys Ala Gly Val Cys 485 490

Claims

1. A method for producing a plant with altered seed yield, the method comprising transformation of a plant with: a) a polynucleotide encoding of a polypeptide with the amino acid sequence of SEQ ID NO:1 or a variant of the polypeptide, wherein the variant is capable of modulating seed yield in a plant; b) a polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a), or c) a polynucleotide comprising a compliment, of at least 15 nucleotides in length, of the polynucleotide of a).

2. The method of claim 1 in which the variant has at least 70% sequence identity to a polypeptide with the amino acid sequence of SEQ ID NO: 1.

3. The method of claim 1 or claim 2 in which the variant is derived from a plant species and comprises the amino acid sequence of SEQ ID NO: 32.

4. The method of any one of claims 1 to 3 in which the variant is derived from a monocotyledonous species and comprises the amino acid sequence of SEQ ID NO: 33.

5. The method of any one of claims 1 to 3 in which the variant comprises an amino acid sequence selected from any one of SEQ ID NO: 2-31.

6. The method of any one of claims 1 to 5 in which the variant is a glutamate decarboxylase.

7. The method of claim 1 in which the polynucleotide of a) encodes a polypeptide with the amino acid sequence of SEQ ID NO: 1.

8. A method of producing a plant with altered seed yield, the method comprising transformation of a plant cell or plant with: a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 34, or a variant thereof, wherein the variant encodes a polypeptide capable of modulating seed yield in a plant; b) a polynucleotide comprising a fragment, of at least 15 nucleotides in length, of the polynucleotide of a), or c) a polynucleotide comprising a complement, of at least 15 nucleotides in length, of the polynucleotide of a).

9. The method of claim 8 in which the variant has at least 70% sequence identity to SEQ ID NO: 34.

10. The method of claim 8 in which the variant has at least 70% sequence identity to the coding sequence of SEQ ID NO: 34.

11. The method of claim 8 in which the variant comprises the sequence of any one of SEQ ID NO: 35 to 65.

12. The method of claim 8 in which the variant comprises the coding sequence of any one of SEQ ID NO: 35 to 65.

13. The method of any one of claims 8 to 12 in which the polypeptide is a glutamate decarboxylase.

14. The method of claim 8 in which the polynucleotide of a) comprises the sequence of SEQ ID NO: 34.

15. The method of claim 8 in which the polynucleotide of a) comprises the coding sequence of SEQ ID NO: 34.

16. The method of any one of claims 1 to 15 in which the plant produced has increased seed yield relative to a suitable control plant.

17. A plant cell or plant produced by a method of any one of claims 1 to 16.

18. An isolated polynucleotide encoding a polypeptide with at least 75% sequence identity to the amino acid sequence of SEQ ID NO: 1, wherein the polynucleotide encodes a polypeptide capable of modulating seed yield in a plant.

19. The isolated polynucleotide of claim 18 wherein the polypeptide is a glutamate decarboxylase.

20. The isolated polynucleotide of claim 18 comprising the sequence of SEQ ID NO: 34.

21. The isolated polynucleotide of claim 18 comprising coding sequence of SEQ ID NO: 34.

22. An isolated polypeptide having at least 83% sequence identity to the amino acid sequence of SEQ ID NO: 1, wherein the polypeptide is capable of modulating seed yield in a plant.

23. The isolated polypeptide of claim 22, wherein the polypeptide is a glutamate decarboxylase.

24. The isolated polypeptide of claim 22 comprising the amino acid sequence of SEQ ID NO: 1.

25. A genetic construct which comprises a polynucleotide of any one of claims 18 to 21.

26. A genetic construct including a polynucleotide consisting of at least one of: a) a fragment, of at least 15 nucleotides in length, of a polynucleotide of any one of claims 18 to 21; b) a complement, of at least 15 nucleotides in length, of the polynucleotide of any one of claims 18 to 21; or c) a sequence, of at least 15 nucleotides in length, capable of hybridising to the polynucleotide of any one of claims 18 to 21.

27. The genetic construct of claim 26 comprising a promoter sequence operably linked to the polynucleotide.

28. The genetic construct of claim 26 or 27 comprising a terminator sequence operably linked to the polynucleotide.

29. A host cell genetically modified to express a polynucleotide of any one of claims 18 to 21, or a polypeptide of any one of claims 22 to 24.

30. A host cell comprising a genetic construct any one of claims 25 to 28.

31. A plant cell genetically modified to express a polynucleotide of any one of claims 18 to 21, or a polypeptide of any one of claim 22 or 24.

32. A plant cell which comprises a genetic construct any one of claims 25 to 28.

33. A plant which comprises a plant cell of claim 31 or 32.

34. A method for selecting a plant with altered seed yield relative to suitable control plant, the method comprising testing of a plant for altered expression of a polynucleotide of any one of claims 18 to 21.

35. A method for selecting a plant with altered seed yield relative to a suitable control plant, the method comprising testing of a plant for altered expression of a polypeptide of claims 22 to 24.

36. A group of plants selected by the method of claim 34 or 35.

37. An antibody raised against a polypeptide of any one of claims 22 to 24.