Selecting animals for parentally imprinted traits

Abstract

selecting a domestic animal having desired genotypic properties, the methods comprising testing the animal for the presence of a parentally imprinted quantitative trait locus (QTL). The invention further relates to the use of an isolated and/or recombinant nucleic acid comprising a QTL or functional fragment derived therefrom to select a breeding animal or animal destined for slaughter having desired genotypic or potential phenotypic properties. In particular, the properties are related to muscle mass, fat deposition, sow prolificacy, and/or sow longevity.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001]This application is a divisional of co-pending U.S. patent application Ser. No. 11/357,733, filed Feb. 17, 2006, which is a continuation in part of U.S. patent application Ser. No. 09/868,732, filed on Nov. 1, 2001, now U.S. Pat. No. 7,255,987, issued Aug. 14, 2007, which is a national stage entry under 35 U.S.C. §371 of International Application No. PCT/EP99/10209, filed on Dec. 16, 1999, and published in English as International Publication No. WO 00/36143, on Jun. 22, 2000, which claims the benefit under Article 8 of the Patent Cooperation Treaty of European Patent Application EP 98204291.3, filed on Dec. 16, 1998, the contents of the entirety of each of which are incorporated herein by this reference.

STATEMENT ACCORDING TO 37 C.F.R. §1.52(e)(5)

Sequence Listing Submitted on Compact Disc

[0002]Pursuant to 37 C.F.R. §1.52(e)(1)(iii), a compact disc containing an electronic version of the Sequence Listing has been submitted concomitant with this application, the contents of which are hereby incorporated by reference. A second compact disc is submitted and is an identical copy of the first compact disc. The discs are labeled "copy 1" and "copy 2," respectively, and each disc contains one file entitled "2183-7695US Sequence Listing.ST25.txt" which is 170 KB and created on Jul. 17, 2006.

TECHNICAL FIELD

[0003]The invention relates generally to biotechnology and, more particularly, to methods for selecting breeding animals or animals destined for slaughter having desired genotypic or potential phenotypic properties, in particular, related to muscle mass and/or fat deposition.

BACKGROUND

[0004]Breeding schemes for domestic animals have so far focused on farm performance traits and carcass quality. This has resulted in substantial improvements in traits like reproductive success, milk production, lean/fat ratio, prolificacy, growth rate and feed efficiency. Relatively simple performance test data have been the basis for these improvements, and selected traits were assumed to be influenced by a large number of genes, each of small effect (the infinitesimal gene model). There are now some important changes occurring in this area. First, the breeding goal of some breeding organizations has begun to include meat quality attributes in addition to the "traditional" production traits. Secondly, evidence is accumulating that current and new breeding goal traits may involve relatively large effects (known as major genes), as opposed to the infinitesimal model that has been relied on so far.

[0005]Modern DNA-technologies provide the opportunity to exploit these major genes, and this approach is a very promising route for the improvement of meat quality, especially since direct meat quality assessment is not viable for potential breeding animals. Also for other traits such as lean/fat ratio, growth rate and feed efficiency, modern DNA technology can be very effective. Also these traits are not always easy to measure in the living animal.

[0006]The evidence for several of the major genes was originally obtained using segregation analysis, i.e., without any DNA marker information. Afterwards, molecular studies were performed to detect the location of these genes on the genetic map. In practice, and except for alleles of very large effect, DNA studies are required to dissect the genetic nature of most traits of economic importance. DNA markers can be used to localize genes or alleles responsible for qualitative traits like coat color, and they can also be used to detect genes or alleles with substantial effects on quantitative traits like growth rate, IMF etc. In this case, the approach is referred to as QTL (quantitative trait locus) mapping, wherein a QTL comprises at least a part of the nucleic acid genome of an animal where genetic information capable of influencing the quantitative trait (in the animal or in its offspring) is located. Information at DNA level can not only help to fix a specific major gene in a population, but also assist in the selection of a quantitative trait which is already selected for. Molecular information in addition to phenotypic data can increase the accuracy of selection and therefore the selection response.

[0007]Improving meat quality or carcass quality is not just about changing levels of traits like tenderness or marbling, but it is also about increasing uniformity. The existence of major genes provides excellent opportunities for improving meat quality because it allows large steps to be made in the desired direction. Secondly, it will help to reduce variation, since we can fix relevant genes in our products. Another aspect is that selecting for major genes allows differentiation for specific markets. Studies are underway in several species, particularly, pigs, sheep, deer and beef cattle.

[0008]In particular, intense selection for meat production has resulted in animals with extreme muscularity and leanness in several livestock species. In recent years, it has become feasible to map and clone several of the genes causing these phenotypes, paving the way towards more efficient marker-assisted selection, targeted drug development (performance enhancing products) and transgenesis. Mutations in the ryanodine receptor (Fuji et al., 1991; MacLennan and Phillips, 1993) and myostatin (Grobet et al., 1997; Kambadur et al., 1997; McPherron and Lee, 1997) have been shown to cause muscular hypertrophies in pigs and cattle respectively, while genes with major effects on muscularity and/or fat deposition have, for instance, been mapped to pig chromosome 4 (Andersson et al., 1994) and sheep chromosome 18 (Cocket et al., 1996).

[0009]However, although there have been successes in identifying QTLs, the information is currently of limited use within commercial breeding programs. Many workers in this field conclude that it is necessary to identify the particular genes underlying the QTL. This is a substantial task, as the QTL region is usually relatively large and may contain many genes. Identification of the relevant genes from the many that may be involved thus remains a significant hurdle in farm animals.

SUMMARY OF THE INVENTION

[0010]In one aspect, provided is a method for selecting a domestic animal for having desired genotypic or potential phenotypic properties, the method comprising testing the animal for the presence of a parentally imprinted qualitative or quantitative trait locus (QTL). Herein, a domestic animal is defined as an animal being selected or having been derived from an animal having been selected for having desired genotypic or potential phenotypic properties.

[0011]Domestic animals provide a rich resource of genetic and phenotypic variation; traditionally, domestication involves selecting an animal or its offspring for having desired genotypic or potential phenotypic properties. This selection process has in the past century been facilitated by a growing understanding and utilization of the laws of Mendelian inheritance. One of the major problems in breeding programs of domestic animals is the negative genetic correlation between reproductive capacity and production traits. This is, for example, the case in cattle (a high milk production generally results in slim cows and bulls) poultry, broiler lines have a low level of egg production and layers have generally very low muscle growth), pigs (very prolific sows are, in general, fat and have comparatively less meat) or sheep (high prolific breeds have low carcass quality and vice versa). The invention now provides that knowledge of the parental imprinting character of various traits allows selection of, for example, sire lines, homozygous for a paternally imprinted QTL, for example, linked with muscle production or growth; the selection for such traits can thus be less stringent in dam lines in favor of the reproductive quality. The phenomenon of genetic or parental imprinting has never been utilized in selecting domestic animals, it was never considered feasible to employ this elusive genetic characteristic in practical breeding programs. Provided is a breeding program, wherein knowledge of the parental imprinting character of a desired trait, as demonstrated herein, results in a breeding program, for example in a BLUP program, with a modified animal model. This increases the accuracy of the breeding value estimation and speeds up selection compared to conventional breeding programs. Until now, the effect of a parentally imprinted trait in the estimation of a conventional BLUP program was neglected; using and understanding the parental character of the desired trait, as provided by the invention, allows selecting on parental imprinting, even without DNA testing. For example, selecting genes characterized by paternal imprinting is provided to help increase uniformity; a (terminal) parent homozygous for the "good or wanted" alleles will pass them to all offspring, regardless of the other parent's alleles, and the offspring will all express the desired parent's alleles. This results in more uniform offspring. Alleles that are interesting or favorable from the maternal side are often the ones that have opposite effects to alleles from the paternal side. For example, in meat animals, such as pigs, alleles linked with meat quality traits, such as intra-muscular fat or muscle mass, could be fixed in the dam lines while alleles linked with reduced back fat could be fixed in the sire lines. Other desirable combinations are, for example, fertility and/or milk yield in the female line with growth rates and/or muscle mass in the male lines.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

[0012]FIG. 1: Test statistic curves obtained in QTL analyses of chromosome 2 in a Wild Boar/Large White intercross. The graph plots the F ratio testing the hypothesis of a single QTL at a given position along the chromosome for the traits indicated. The marker map with the distances between markers in Kosambi centiMorgan is given on the X-axis. The horizontal lines represent genome-wise significant (P<0.05) and suggestive levels for the trait lean meat in ham; similar significance thresholds were obtained for the other traits.

[0013]FIG. 2: Pietrain pig with characteristic muscular hypertrophy.

[0014]FIG. 3: Lodscore curves obtained in a Pietrain x Large White intercross for six phenotypes measuring muscle mass and fat deposition on pig chromosome 2. The most likely positions of the Igf2 and MyoD genes determined by linkage analysis with respect to the microsatellite marker map are shown. H₀ was defined as the null-hypothesis of no QTL, H₁ as testing for the presence of a Mendelian QTL, H₂ as testing for the presence of a paternally expressed QTL, and H₃ as testing for the presence of a maternally expressed QTL. FIG. 3A: log₁₀(H₁/H₀), FIG. 3B: log₁₀ (H₂/H₀), FIG. 3C: log₁₀ (H₃/H₀).

[0015]FIG. 4: Panel A. Structure of the human Igf2 gene according to reference 17, with aligned porcine adult liver cDNA sequence as reported in reference 16. The position of the nt241(G-A) transition and Swc9 microsatellite are shown. Panel B. The corresponding markers were used to demonstrate the monoallelic (paternal) expression of Igf2 in skeletal muscle and liver of ten-week-old fetuses. PCR amplification of the nt421(G-A) polymorphism and Swc9 microsatellite from genomic DNA clearly shows the heterozygosity of the fetus, while only the paternal allele is detected in liver cDNA (nt421(G-A) and Swc9) and muscle cDNA (Swc9). The absence of RT-PCR product for nt421(G-A) from in-fetal muscle points towards the absence of mRNA including exon 2 in this tissue. Parental origin of the fetal alleles was determined from the genotypes of sire and dam (data not shown).

[0016]FIG. 5: A NotI restriction map showing the relative position of BAC-PIGF2-1 (comprising INS and IGF2 genes), and BAC-PIGF2-2 (comprising IGF2 and H19 genes).

[0017]FIG. 6: Nucleic acid sequences of contig 1 to contig 115 derived from BAC-PIGF2-1, which was shotgun sequenced using standard procedures and automatic sequencers (SEQ ID NOS:10-117).

[0018]FIG. 7: Similarity between porcine contigs of FIG. 6 and orthologous sequences in human.

[0019]FIG. 8: Nucleic acid sequences of contig 1 to contig 7 derived from BAC-PIGF2-2, (the 24 Kb NotI fragment not present in BAC-PIGF2-1) which was subcloned and sequenced using the EZ::TN transposon approach and ABI automatic sequencers (SEQ ID NOS:118-124).

[0020]FIG. 9: Similarity between porcine contigs of FIG. 8 and orthologous sequences in human.

[0021]FIG. 10: DNA sequence polymorphisms in the IGF2 and flanking loci from genomic DNA isolated from Pietrain, Large White and Wild Boar individuals. (Polymorphisms Tyrosine Hydroxylase Gene C3 (1-4) (SEQ ID NOS:128-132), Polymorphism Insulin-IGF2 C4 (5-23) (SEQ ID NOS:133-170), Polymorphismes in Coding Region C10 (24-28) (SEQ ID NOS:171-180), Microsatellites (29-31) (SEQ ID NOS:181-183).

[0022]FIG. 11: Representation of a suitable marker-assisted selection program for the IGF2 mutation.

DETAILED DESCRIPTION OF THE INVENTION

[0023]In certain embodiments, provided is a method for selecting a domestic animal for having desired genotypic or potential phenotypic properties, the method comprising testing a nucleic acid sample from the animal for the presence of a parentally imprinted quantitative trait locus (QTL). A nucleic acid sample can, in general, be obtained from various parts of the animal's body by methods known in the art. Traditional samples for the purpose of nucleic acid testing are blood samples or skin or mucosal surface samples, but samples from other tissues can be used as well, particularly, sperm samples, oocyte or embryo samples can be used. In such a sample, the presence and/or sequence of a specific nucleic acid, be it DNA or RNA, can be determined with methods known in the art, such as hybridization or nucleic acid amplification or sequencing techniques known in the art. Provided is testing such a sample for the presence of nucleic acid wherein a QTL or allele associated therewith is associated with the phenomenon of parental imprinting, for example where it is determined whether a paternal or maternal allele of the QTL is capable of being predominantly expressed in the animal.

[0024]The purpose of breeding programs in livestock is to enhance the performances of animals by improving their genetic composition. In essence, this improvement accrues by increasing the frequency of the most favorable alleles for the genes influencing the performance characteristics of interest. These genes are referred to as QTL. Until the beginning of the nineties, genetic improvement was achieved via the use of biometrical methods, but without molecular knowledge of the underlying QTL.

[0025]Since the beginning of the Nineties, and due to recent developments in genomics, it is conceivable to identify the QTL underlying a trait of interest. The invention now provides identifying and using parentally imprinted QTLs which are useful for selecting animals by mapping quantitative trait loci. Again, the phenomenon of genetic or paternal imprinting has never been utilized in selecting domestic animals, it was never considered feasible to employ this elusive genetic characteristic in practical breeding programs. For example, Kovacs and Kloting (Biochem. Mol. Biol. Int. 44:399-405, 1998), where parental imprinting is not mentioned, and not suggested, found linkage of a trait in female rats, but not in males, suggesting a possible sex specificity associated with a chromosomal region, which of course excludes parental imprinting, a phenomenon wherein the imprinted trait of one parent is preferably but gender-aspecifically expressed in his or her offspring.

[0026]Provided is the initial localization of a parentally imprinted QTL on the genome by linkage analysis with genetic markers, and the actual identification of the parentally imprinted gene(s) and causal mutations therein. Molecular knowledge of such a parentally imprinted QTL allows for more efficient breeding designs herewith provided. Applications of molecular knowledge of parentally imprinted QTLs in breeding programs include: marker-assisted segregation analysis to identify the segregation of functionally distinct parentally imprinted QTL alleles in the populations of interest, marker-assisted selection (MAS) performed within lines to enhance genetic response by increasing selection accuracy, selection intensity or by reducing the generation interval using the understanding of the phenomenon of parental imprinting, marker-assisted introgression (MAI) to efficiently transfer favorable parentally imprinted QTL alleles from a donor to a recipient population, genetic engineering of the identified parentally QTL and genetic modification of the breeding stock using transgenic technology, development of performance enhancing products using targeted drug development exploiting molecular knowledge of the QTL.

[0027]The inventors herein undertook two independent experiments to determine the practical use of parental imprinting of a QTL.

[0028]In a first experiment, performed in a previously described Pietrain x Large White intercross, the likelihood of the data was computed under a model of paternal (paternal allele only expressed) and maternal imprinting (maternal allele only expressed) and compared with the likelihood of the data under a model of a conventional "Mendelian" QTL. The results strikingly demonstrated that the QTL was indeed paternally expressed, the QTL allele (Pietrain or Large White) inherited from the F1 sow having no affect whatsoever on the carcass quality and quantity of the F₂ offspring. It was seen that very significant lodscores were obtained when testing for the presence of a paternally expressed QTL, while there was no evidence at all for the segregation of a QTL when studying the chromosomes transmitted by the sows. The same tendency was observed for all traits showing that the same imprinted gene is responsible for the effects observed on the different traits. Table 1 reports the maximum likelihood (ML) phenotypic means for the F₂ offspring sorted by inherited paternal QTL allele.

[0029]In a second experiment performed in the Wild Boar X Large White intercross, QTL analyses of body composition, fatness, meat quality, and growth traits was carried out with the chromosome 2 map using a statistical model testing for the presence of an imprinting effect. Clear evidence for a paternally expressed QTL located at the very distal tip of 2p was obtained (FIG. 2; Table 1). The clear paternal expression of a QTL is illustrated by the least squares means which fall into two classes following the population origin of the paternally inherited allele (Table 1). For a given paternally imprinted QTL, implementation of marker-assisted segregation analysis, selection (MAS) and introgression (MAI), can be performed using genetic markers that are linked to the QTL, genetic markers that are in linkage disequilibrium with the QTL, or using the actual causal mutations within the QTL.

[0030]Understanding the parent-of-origin effect characterizing a QTL allows for its optimal use in breeding programs. Indeed, marker-assisted segregation analysis under a model of parental imprinting will yield better estimates of QTL allele effects. Moreover, it allows for the application of specific breeding schemes to optimally exploit a QTL. In one embodiment of the invention, the most favorable QTL alleles would be fixed in breeding animal lines and, for example, used to generate commercial, crossbred males by marker-assisted selection (MAS, within lines) and marker-assisted introgression (MAI, between lines). In another embodiment, the worst QTL alleles would be fixed in the animal lines used to generate commercial crossbred females by MAS (within lines) and MAI (between lines).

[0031]In certain embodiments of the invention, the animal is a pig. Note, for example, that provided is the insight that today half of the offspring from commercially popular Pietrain_x Large White crossbred boars inherit an unfavorable Large White muscle mass QTL as provided by the invention causing considerable loss, and the invention now, for example, provides the possibility to select the better half of the population in that respect. However, it is also possible to select commercial sow lines enriched with the boar's unfavorable alleles, allowing to equip the sows with other alleles that are more desirable, for example, for reproductive purposes.

[0032]In certain embodiments of a method provided by the invention, the QTL is located at a position corresponding to a QTL located at chromosome 2 in the pig. For example, it is known from comparative mapping data between pig and human, including bidirectional chromosome painting, that SSC2p is homologous to HSA11pter-q13.¹¹, 12 HSA11pter-q13 is known to harbor a cluster of imprinted genes: IGF2, INS2, H19, MAH2, P57.sup.KIP2, K_vLQTL1, Tapa1/CD81, Orctl2, Impt1 and Ip1. The cluster of imprinted genes located in HSA11pter-q13 is characterized by eight maternally expressed genes H19, MASH2, P57.sup.KIP2, K_vLQTL1, TAPA1/CD81, ORCTL2, IMPT1 and IP1, and two paternally expressed genes: IGF2 and INS. However, Johanson et al. (Genomics 25:682-690, 1995) and Reik et al. (Trends in Genetics 13:330-334, 1997) show that the whereabouts of these loci in various animals are not clear. For example, the HSA11 and MMU7 loci do not correspond among each other, the MMU7 and the SSC2 loci do not correspond, whereas the HSA11 and SSC2 loci seem to correspond, and no guidance is given where one or more of, for example, the above-identified parentally expressed individual genes are localized on the three species' chromosomes.

[0033]Other domestic animals, such as cattle, sheep, poultry and fish, having similar regions in their genome harboring such a cluster of imprinted genes or QTLs, provided is the use of these orthologous regions of other domestic animals in applying the phenomenon of parental imprinting in breeding programs. In pigs, the cluster is mapped at around position 2p1.7 of chromosome 2, however, a method as provided by the invention employing (fragments of) the maternally or paternally expressed orthologous or homologous genes or QTLs are advantageously used in other animals as well for breeding and selecting purposes. For example, a method is provided wherein the QTL is related to the potential muscle mass and/or fat deposition, e.g., with limited effects on other traits, such as meat quality and daily gain of the animal, or wherein the QTL comprises at least a part of an insulin-like growth factor-2 (IGF2) allele. Reik et al. (Trends in Genetics 13:330-334, 1997) explain that this gene in humans is related to Beckwith-Wiedemann syndrome, an apparently parentally imprinted disease syndrome most commonly seen with human fetuses, where the gene has an important role in prenatal development. No relationship is shown or suggested with postnatal development relating to muscle development or fatness in animals.

[0034]In certain embodiments, provided is a method for selecting a pig for having desired genotypic or potential phenotypic properties, the method comprising testing a sample from the pig for the presence of a quantitative trait locus (QTL) located at a Sus scrofa chromosome 2 mapping at position 2p1.7. In particular, the methods relate to the use of genetic markers for the telomeric end of pig chromosome 2p in marker selection (MAS) of a parentally imprinted Quantitative Trait Locus (QTL) affecting carcass yield and quality in pigs. Furthermore, the methods relate to the use of genetic markers associated with the IGF2 locus in MAS in pigs, such as polymorphisms and microsatellites and other characterizing nucleic acid sequences shown herein, such as shown in FIGS. 4 to 10. In certain embodiments, provided is a QTL located at the distal tip of Sus scrofa chromosomes 2 with effects on varies measurements of carcass quality and quantity, particularly muscle mass and fat deposition.

[0035]In a first experiment, a QTL mapping analysis was performed in a Wild Boar X Large White intercross counting 200 F₂ individuals. The F₂ animals were sacrificed at a live eight of at least 80 kg or at a maximum age of 190 days. Phenotypic data on birth weight, growth, fat deposition, body composition, weight of internal organs, and meat quality were collected; a detailed description of the phenotypic traits are provided by Andersson et al.¹ and Andersson-Eklund et al.⁴

[0036]A QTL (without any significant effect on back-fat thickness) at an unspecified locus on the proximal end of chromosome 2 with moderate effect on muscle mass, and located about 30 cm away from the parentally imprinted QTL reported here, was previously reported by the inventors; whereas the QTL as now provided has a very large effect, explaining at least 20-30% of variance, making the QTL of the present invention commercially very attractive, which is even more so because the present QTL is parentally imprinted. The marker map of chromosome 2p was improved as part of this invention by adding microsatellite markers in order to cover the entire chromosome arm. The following microsatellite markers were used: Swc9, Sw2443, Sw2623, and Swr2516, all from the distal end of 2p.⁷ QTL analyses of body composition, fatness, meat quality, and growth traits were carried out with the new chromosome 2 map. Clear evidence for a QTL located at the very distal tip of 2p was obtained (FIG. 1; Table 1). The QTL had very large effects on lean meat content in ham and explained an astonishing 30% of the residual phenotypic variance in the F₂ population. Large effects on the area of the longissumus dorsi muscle, on the weight of the heart, and on back-fat thickness (subcutaneous fat) were also noted. A moderate effect on one meat quality trait, reflectance value, was indicated. The QTL had no significant effect on abdominal fat, birth weight, growth, weight of liver, kidney, or spleen (data not shown). The Large White allele at this QTL was associated with larger muscle mass and reduced back-fat thickness consistent with the difference between this breed and the Wild Boar population.

[0037]In a second experiment, QTL mapping was performed in a Pietrain X Large White intercross comprising 1125 F₂ offspring. The Large White and Pietrain parental breeds differ for a number of economically important phenotypes. Pietrains are famous for their exceptional muscularity and leanness¹⁰ (FIG. 2, while Large Whites show superior growth performance. Twenty-one distinct phenotypes measuring growth performance (5), muscularity (6), fat deposition (6), and meat quality (4), were recorded on all F₂ offspring. In order to map QTL underlying the genetic differences between these breeds, the inventors undertook a whole genome scan using microsatellite markers on an initial sample of 677 F₂ individuals. The following microsatellite marker map was used to analyze chromosome 2;:SW2443, SWC9 and SW2623, SWR2516-(0,20)-SWR783-(0,29)-SW240-(0,20)-SW776-(0,08)-S0010-(0,04)-SW169- 5-(0,36)-SW R308. Analysis of pig chromosome 2 using a Maximum Likelihood multipoint algorithm, revealed highly significant lodscores (up to 20) for three of the six phenotypes measuring muscularity (% lean cuts, % ham, % loin) and three of the six phenotypes measuring fat deposition (back-fat thickness (BFT), % back fat, % fat cuts) at the distal end of the short arm of chromosome 2 (FIG. 1). Positive lodscores were obtained in the corresponding chromosome region for the remaining six muscularity and fatness phenotypes, however, not reaching the experiment-wise significance threshold) (a=5%. There was no evidence for an effect of the corresponding QTL on growth performance (including birth weight) or recorded meat quality measurements (data not shown). To confirm this finding, the remaining sample of 355 F₂ offspring was genotyped for the four most distal 2p markers and QTL analysis performed for the traits yielding the highest lodscores in the first analysis. Lodscores ranged from 2.1 to 7.7, clearly confirming the presence of a major QTL in this region. Table 2 reports the corresponding ML estimates for the three genotypic means as well as the residual variance. Evidence based on marker-assisted segregation analysis points towards residual segregation at this locus within the Pietrain population.

[0038]These experiments therefore clearly indicated the existence of a QTL with major effect on carcass quality and quantity on the telomeric end of pig chromosome arm 2p; the likely existence of an allelic series at this QTL with at least three alleles: Wild-Boar<Large White<Pietrain, and possibly more given the observed segregation within the Pietrain breed.

[0039]The effects of the identified QTL on muscle mass and fat deposition are truly major, being of the same magnitude of those reported for the CRC locus though apparently without the associated deleterious effects on meat quality. We estimate that both loci jointly explain close to 50% of the Pietrain versus Large White breed difference for muscularity and leanness. The QTL had very large effects on lean meat content in ham and explained an astonishing 30% of the residual phenotypic variance in the F₂ population. Large effects on the area of the longissumus dorsi muscle, on the weight of the heart, and on back-fat thickness (subcutaneous fat) were also noted. A moderate effect on one meat quality trait, reflectance value, was indicated. The QTL had no significant effect on abdominal fat, birth weight, growth, weight of liver, kidney, or spleen (data not shown). The Large White allele at this QTL, when compared to the Wild Boar allele, was associated with larger muscle mass and reduced back-fat thickness consistent with the difference between this breed and the Wild Boar population. The strong imprinting effect observed for all affected traits shows that a single causative locus is involved. The pleiotropic effects on skeletal muscle mass and the size of the heart appear adaptive from a physiological point of view as a larger muscle mass requires a larger cardiac output.

[0040]In a further embodiment, provided is a method for selecting a pig for having desired genotypic or potential phenotypic properties comprising testing a sample from the pig for the presence of a quantitative trait locus (QTL) located at a Sus scrofa chromosome 2 mapping at position 2p1.7., wherein the QTL comprises at least a part of a Sus scrofa insulin-like growth factor-2 (IGF2) allele or a genomic area closely related thereto, such as polymorphisms and microsatellites and other characterizing nucleic acid sequences shown herein, such as shown in FIGS. 4 to 10. The important role of IGF2 for prenatal development is well-documented from knock-out mice as well as from its causative role in the human Beckwith-Wiedemann syndrome. This invention demonstrates an important role for the IGF2-region also for postnatal development.

[0041]To show the role of Igf2, the inventors performed the following three experiments:

[0042]A genomic IGF2 clone was isolated by screening a porcine BAC library. FISH analysis with this BAC clone gave a strong consistent signal on the terminal part of chromosome 2p.

[0043]A polymorphic microsatellite is located in the 3'UTR of IGF2 in mice (GenBank U71085), humans (GenBank S62623), and horse (GenBank AF020598). The possible presence of a corresponding porcine microsatellite was investigated by direct sequencing of the IFG2 3'UTR using the BAC clone. A complex microsatellite was identified about 800 bp downstream of the stop codon; a sequence comparison revealed that this microsatellite was identical to a previously described anonymous microsatellite, Swc9.⁶ This marker was used in the initial QTL mapping experiments and its location on the genetic map correspond with the most likely position of the QTL both in the Pietrain X Large White and in the Large White x Wild Boar pedigree.

[0044]Analysis of skeletal muscle and liver cDNA from ten-week-old fetuses heterozygous for a nt241 (G-A) transversion in the second exon of the porcine IGFII gene and SWC9, shows that the IGFII gene is imprinted in these tissues in the pig as well and only expressed from the paternal allele.

[0045]Based on a published porcine adult liver cDNA sequence,¹⁶ the inventors designed primer pairs allowing amplification of the entire IgfII coding sequence with 222 by of leader and 280 by of trailer sequence from adult skeletal muscle cDNA. Pietrain and Large White RT-PCR products were sequenced indication that the coding sequences are identical in both breeds and with the published sequence. However, a GA transition was found in the leader sequence corresponding to exon 2 in man. Following conventional nomenclature, this polymorphism will be referred to as nt241(G-A). We developed a screening test for this single nucleotide polymorphism 9(SNP) based on the ligation amplification reaction (LAR), allowing us to genotype our pedigree material. Based on these data, IgfII was shown to colocalize with the SWC9 microsatellite marker (0=0%), therefore virtually coinciding with the most likely position of the QTL, and well within the 95% support interval for the QTL. Subsequent sequence analysis demonstrated that the microsatellite marker SWC9 is actually located within the 3'UTR of the IgfII gene.

[0046]As previously mentioned, the knowledge of this QTL provides a method for the selection of animals such as pigs with improved carcass merit. Different embodiments of the invention are envisaged, including: marker-assisted segregation analysis to identify the segregation of functionally distinct QTL alleles in the populations of interest; marker-assisted selection (MAS) performed within lines to enhance genetic response by increasing selection accuracy, selection intensity or by reducing the generation interval; marker-assisted introgression (MAI) to efficiently transfer favorable QTL alleles from a donor to a recipient population, thereby enhancing genetic response in the recipient population. Implementation of embodiments marker-assisted segregation analysis, selection (MAS) and introgression (MAI), can be performed using genetic markers that are linked to the QTL; genetic markers that are in linkage disequilibrium with the QTL, the actual causal mutations within the QTL.

[0047]As indicated above, the insulin-like growth factor 2 (IGF2) gene was mapped to the distal tip of the short arm on chromosome 2 in swine. Gene mapping studies indicated that this paternally expressed QTL at the IGF2 gene region has a large effect on back fat thickness and carcass leanness (e.g., Jeon et al. (1999), Nature Genetics 21, 157-158; Nezer et al. (1999), Nature Genetics 21, 155-156). Recently, a mutation in the regulatory region of the IGF2 gene has been identified to be the cause underlying the QTL effect on muscle growth and fat deposition (Van Laere et al. (2003) Nature 425:832-836). This single nucleotide substitution (G-A), located at position 3072 in the intron 3 of IGF2 gene, increases gene expression of IGF2 in muscle threefold, stimulates muscle growth at the expense of back fat and results in leaner swine carcass and lower back fat.

[0048]The large effect of the QTL on lean meat and back fat without influence on growth or meat quality, makes this an attractive QTL to use in the breeding program. Terminal sires have been selected to be homozygous for the lean allele (AA) in order to pass the full effect to their offspring. Field results have been reported by several authors (Scheller et al. (2002) Proceedings of the 27th Annual National Swine Improvement Federation, Des Moines, Iowa, USA; Buys, N. (2003) Proceedings of the 28th Annual National Swine Improvement Federation, Des Moines, Iowa, USA, pp 146-149).

[0049]It is generally believed that prolificacy and sow longevity is reducing as a result of the genetic selection for increased leanness and lowering fat deposition (See, e.g., P. Mathur and Y. Liu (2003), Proceedings of the 28th Annual National Swine Improvement Federation, Des Moines, Iowa, USA, pp 155-163). Body fat deposition is necessary to sustain sow reproduction performance, for example, to support adequate milk production and to limit body weight loss. The selection for leaner carcasses, demanded by the packing industry and consumers, may conflict with the prolificacy and longevity of the sow and lead to increased replacement costs of sows in pig production. The QTN (quantitative trait nucleotide) in the IGF2 gene might provide a possibility to overcome this conflict. The imprinting character of the gene might be used to produce lean slaughter pigs from fatter dams, that inherited the wild type allele from their father (genotype of Grand parent boar=GG), crossed with terminal sires being homozygous for the lean allele (AA). The objective of this experiment was to investigate a possible effect of the QTN at the IGF2 gene on prolificacy and longevity.

[0050]The details of the experiment are described in Example 5. It was found that sows that inherited the wild type allele from their father had significantly more piglets born alive, total born and weaned, while there was no effect on stillborn piglets (See, Example 5, Table 4). No effect on any of these prolificacy data could be observed when data were analyzed according to the allele inherited from the mother (maternal allele). Also, if sows from heterozygous dams were taken into account and grouped according to the maternal allele, again no significant effect on prolificacy could be observed, which was expected since the maternal allele is not expressed.

[0051]The parity or average number of cycles per sow was also found to be higher in sows that inherited G from their father as compared to those that received the A allele, which points to a beneficial effect on longevity. This is related to higher litter size, since that is a major criterion for elimination in the selection program.

[0052]Thus, the IGF2-intron3 G3072A mutation (herein also referred to as IGF2+ or A-allele; the wild type being the igf2- or G-allele) has an influence on prolificacy and longevity in sows, which allows for the possibility to use the same imprinted QTN for different selection in sire and dam lines. Terminal sires should be homozygous for the lean allele to give uniform and lean slaughter pigs, while dam lines can benefit from a selection for the wild type allele since this has a beneficial effect on prolificacy and longevity. Because of the imprinted character of the gene, selection for the fatter allele in sow lines will not influence the carcass quality of the offspring.

[0053]Thus, provided is a method for selecting a domestic animal for having desired genotypic properties comprising testing the animal for the presence of a parentally imprinted quantitative trait locus (QTL) or a mutation therein and to the use of an isolated and/or recombinant nucleic acid comprising a parentally imprinted quantitative trait locus (QTL) or a mutation therein or functional fragment derived thereof to select a breeding animal or animal destined for slaughter having desired genotypic or potential phenotypic properties. The test may, for instance, comprise testing a sample from the pig for the presence of a quantitative trait locus (QTL) located at a Sus scrofa chromosome 2 mapping at position 2p1.7., wherein the QTL is paternally expressed, i.e., is expressed from the paternal allele.

[0054]In particular, the genotypic or potential phenotypic properties are selected from the group consisting of muscle mass, fat deposition, lean meat, lean back fat, sow prolificacy and sow longevity. In particular, improved sow prolificacy may include such phenotypic expressions as higher teat number, more piglets born alive, higher litter size, higher number of total born and weaned piglets with no effect on stillborn piglets. Improved sow longevity may in particular include such phenotypic expressions as parity or average number of cycles per sow.

[0055]Thus, the above-described IGF2 mutation influencing lean meat also influences a number of other positive traits and allows for marker-assisted selection in opposite directions in sire and dam lines due to the parentally imprinting character of the mutation. The mutation increases muscle mass at the expense of back fat with on average 2-4% more lean meat. This effect on leanness is of the same magnitude as reported for the Halothane gene but without any of the well-known deleterious effects on meat quality and animal health. Homozygous positive terminal sires (IGF2+/IGF2+) will pass the full effect to the slaughter pigs, regardless of the genotype of the mother. Furthermore, such selection principle allows for the possibility to push a far higher proportion of lower grading pigs into the higher payment categories. The experiment described in Example 5 shows that parent sows benefit from inheriting the negative gene (igf2-) from their father: they are more prolific and have an increased longevity. Parent sows are fatter but this will have no effect on the carcass quality of the slaughter pig (see illustration in Example 5).

[0056]In a further embodiment, provided is a method for selecting a pig for having desired genotypic or potential phenotypic properties comprising testing a sample from the pig for the presence of a quantitative trait locus (QTL) located at a Sus scrofa chromosome 2 mapping at position 2p1.7., wherein the QTL is paternally expressed, i.e., is expressed from the paternal allele. In man and mouse, Igf2 is known to be imprinted and to be expressed exclusively from the paternal allele in several tissues. Analysis of skeletal muscle cDNA from pigs heterozygous for the SNP and/or SWC9, shows that the same imprinting holds in the pig as well. Understanding the parent-of-origin effect characterizing the QTL as provided by the invention now allows for its optimal use in breeding programs. Indeed, today half of the offspring from commercially popular Pietrain x Large White crossbred boars inherit the unfavorable Large White allele causing considerable loss. Using a method as provided by the invention avoids this problem.

[0057]Furthermore provided is an isolated and/or recombinant nucleic acid or functional fragment derived thereof comprising a parentally imprinted quantitative trait locus (QTL) or fragment thereof capable of being predominantly expressed by one parental allele. Having such a nucleic acid as provided by the invention available allows constructing transgenic animals wherein favorable genes are capable of being exclusively or predominantly expressed by one parental allele, thereby equipping the offspring of the animal homozygous for a desired trait with desired properties related to that parental allele that is expressed.

[0058]In certain embodiments, provided is an isolated and/or recombinant nucleic acid or fragment derived thereof comprising a synthetic parentally imprinted quantitative trait locus (QTL) or functional fragment thereof derived from at least one chromosome. "Synthetic" herein describes a parentally expressed QTL wherein various elements are combined that originate from distinct locations from the genome of one or more animals. Provided is recombinant nucleic acid wherein sequences related to parental imprinting of one QTL are combined with sequences relating to genes or favorable alleles of a second QTL. Such a gene construct is favorably used to obtain transgenic animals wherein the second QTL has been equipped with paternal imprinting, as opposed to the inheritance pattern in the native animal from which the second QTL is derived. Such a second QTL can, for example, be derived from the same chromosome where the parental imprinting region is located, but can also be derived from a different chromosome from the same or even a different species. In the pig, such a second QTL can, for example, be related to an estrogen receptor (ESR)-gene (Rothschild et al., PNAS 93, 201-201, 1996) or a FAT-QTL (Andersson, Science 263, 1771-1774, 1994) for example derived from another pig chromosome, such as chromosome 4. A second or further QTL can also be derived from another (domestic) animal or a human.

[0059]Furthermore provided is an isolated and/or recombinant nucleic acid or functional fragment derived thereof at least partly corresponding to a QTL of a pig located at a Sus scrofa chromosome 2 mapping at position 2p1.7 wherein the QTL is related to the potential muscle mass and/or fat deposition of the pig and/or wherein the QTL comprises at least a part of a Sus scrofa insulin-like growth factor-2 (IGF2) allele, preferably at least spanning a region between INS and H19, or preferably derived from a domestic pig, such as a Pietrain, Meishan, Duroc, Landrace or Large White, or from a Wild Boar. For example, a genomic IGF2 clone was isolated by screening a porcine BAC library. FISH analysis with this BAC clone gave a strong consistent signal on the terminal part of chromosome 2p. A polymorphic microsatellite is located in the 3'UTR of IGF2 in mice (GenBank U71085), humans (GenBank S62623), and horse (GenBank AF020598). The possible presence of a corresponding porcine microsatellite was investigated by direct sequencing of the IGF2 3'UTR using the BAC clone. A complex microsatellite was identified about 800 by downstream of the stop codon; a sequence comparison revealed that this microsatellite is identical to a previously described anonymous microsatellite, Swc9. PCR primers were designed and the microsatellite (IGF2ms) was found to be highly polymorphic with three different alleles among the two Wild Boar founders and another two among the eight Large White founders. IGF2ms was fully informative in the intercross as the breed of origin as well as the parent of origin could be determined with confidence for each allele in each F₂ animal.

[0060]A linkage analysis using the intercross pedigree was carried out with IGF2ms and the microsatellites Sw2443, Sw2623, and Swr2516, all from the distal end of 2p.⁷ IGF2 was firmly assigned to 2p by highly significant lod scores (e.g., Z=89.0, 0=0.003 against Swr2516). Multipoint analyses, including previously typed chromosome 2 markers, revealed the following order of loci (sex-average map distances in Kosambi cM): Sw2443/Swr2516-0.3-IGF2-14.9-Sw2623-10.3-Sw256. No recombinant was observed between Sw2443 and Swr2516, and the suggested proximal location of IGF2 in relation to these loci is based on a single recombinant giving a lod score support of 0.8 for the reported order. The most distal marker in our previous QTL study, Sw256, is located about 25 cM from the distal end of the linkage group.

[0061]Furthermore provided is use of a nucleic acid or functional fragment derived thereof according to the invention in a method according to the invention. In certain embodiments, use of a method according to the invention is provided to select a breeding animal or animal destined for slaughter, or embryos or semen derived from these animals for having desired genotypic or potential phenotypic properties. In particular, provided is such use wherein the properties are related to muscle mass and/or fat deposition. The QTL as provided by the invention may be exploited or used to improve, for example, lean meat content or back-fat thickness by marker-assisted selection within populations or by marker-assisted introgression of favorable alleles from one population to another. Examples of marker-assisted selection using the QTL as provided by the invention are use of marker-assisted segregation analysis with linked markers or with markers in disequilibrium to identify functionally distinct QTL alleles. Furthermore, identification of a causative mutation in the QTL is now possible, again leading to identifying functionally distinct QTL alleles. Such functionally distinct QTL alleles located at the distal tip of chromosome 2p with large effects on skeletal muscle mass, the size of the heart, and on back-fat thickness are also provided by the invention. The observation of a similar QTL effect in a Large White x Wild Boar as well as in a Pietrain x Large White intercross provides proof of the existence of a series of at least three distinct functional alleles. Moreover, preliminary evidence based on marker-assisted segregation analysis points towards residual segregation at this locus within the Pietrain population (data not shown). The occurrence of an allelic series as provided by the invention allows identifying causal polymorphisms which--based on the quantitative nature of the observed effect--are unlikely to be gross gene alterations but rather subtle regulatory mutations. The effects on muscle mass of the three alleles rank in the same order as the breeds in which they are found, e.g., Pietrain pigs are more muscular than Large White pigs that in turn have higher lean meat content than Wild Boars. Furthermore provided is use of the alleles as provided by the invention for within line selection or for marker-assisted introgression using linked markers, markers in disequilibrium or alleles comprising causative mutations.

[0062]Furthermore provided is an animal selected by using a method according to the invention. For example, a pig characterized in being homozygous for an allele in a QTL located at a Sus scrofa chromosome 2 mapping at position 2p1.7 can now be selected and is thus provided by the invention. Since the QTL is related to the potential muscle mass and/or fat deposition of the pig and/or the QTL comprises at least a part of a Sus scrofa insulin-like growth factor-2 (IGF2) allele, it is possible to select promising pigs to be used for breeding or to be slaughtered. In particular, an animal according to the invention which is a male is provided. Such a male, or its sperm or an embryo derived thereof, can advantageously be used in breeding animals for creating breeding lines or for finally breeding animals destined for slaughter. In certain embodiments of such use as provided by the invention, a male, or its sperm, deliberately selected for being homozygous for an allele causing the extreme muscular hypertrophy and leanness, is used to produce offspring heterozygous for such an allele. Due to the allele's paternal expression, the offspring will also show the favorable traits for example related to muscle mass, even if the parent female has a different genetic background. Moreover, it is now possible to positively select the female(s) for having different traits, for example related to fertility, without having a negative effect on the muscle mass trait that is inherited from the allele from the selected male. For example, earlier such males could occasionally be seen with Pietrain pigs but, genetically, it was not understood how to most profitably use these traits in breeding programs.

[0063]Furthermore, provided is a transgenic animal, sperm and an embryo derived thereof, comprising a synthetic parentally imprinted QTL or functional fragment thereof as provided by the invention, e.g., it is provided by the invention to introduce a favorable recombinant allele; for example, to introduce the estrogen receptor locus related to increased litter size of an animal homozygously in a parentally imprinted region of a grandparent animal (for example, the father of a hybrid sow if the region was paternally imprinted and the grandparent was a boar); to introduce a favorable fat-related allele or muscle mass-related recombinant allele in a paternally imprinted region, and so on. Recombinant alleles that are interesting or favorable from the maternal side are often the ones that have opposite effects to alleles from the paternal side. For example, in meat animals, such as pigs, recombinant alleles linked with meat quality traits such as intra-muscular fat or muscle mass could be fixed in the dam lines while recombinant alleles linked with reduced back fat could be fixed in the sire lines. Other desirable combinations are, for example, fertility and/or milk yield in the female line with growth rates and/or muscle mass in the male lines.

[0064]The invention is further described in the following illustrative Examples.

Example 1

Wild Boar x Large White intercrosses

[0065]Methods: Isolation of an IGF2 BAC clone and fluorescent in situ hybridization (FISH). IGF2 primers (F:5'-GGCAAGTTCTTCCGCTAATGA-3' (SEQ ID NO:1) and R:5'-GCACCGCAGAATTACGACAA-3' (SEQ ID NO:2)) for PCR amplification of a part of the last exon and 3'UTR were designed on the basis of a porcine IGF2 cDNA sequence (GenBank X56094). The primers were used to screen a porcine BAC library and the clone 253G10 was isolated. Crude BAC DNA was prepared as described.²⁴ The BAC DNA was linearized with EcoRV and purified with QIAEXII (QIAGEN GmbH, Germany). The clone was labeled with biotin-14-dATP using the GIBCO-BRL Bionick labeling system (BRL18246-015). Porcine metaphase chromosomes were obtained from pokeweed (Seromed) stimulated lymphocytes using standard techniques. The slides were aged for two days at room temperature and then kept at -20° C. until use. FISH analysis was carried out as previously described.²⁵ The final concentration of the probe in the hybridization mix was 10 ng/ml. Repetitive sequences were suppressed with standard concentrations of porcine genomic DNA. After post-hybridization washing, the biotinylated probe was detected with two layers of avidin-FITC (Vector A-2011). The chromosomes were counterstained with 0.3 mg/ml DAPI (4,6-Diamino-2-phenylindole; Sigma D9542), which produced a G-banding like pattern. No post hybridization banding was needed, since chromosome 2 is easily recognized without banding. A total of 20 metaphase spreads were examined under an Olympus BX-60 fluorescence microscope connected to an IMAC-CCD S30 video camera and equipped with an ISIS 1.65 (Metasystems) software.

[0066]Sequence, Microsatellite, and Linkage Analysis.

[0067]About two μg of linearized and purified BAC DNA was used for direct sequencing with 20 pmoles of primers and BigDye Terminator chemistry (Perkin Elmer, USA). DNA sequencing was done from the 3' end of the last exon towards the 3' end of the UTR until a microsatellite was detected. A primer set (F:5'-GTTTCTCCTGTACCCACACGCATCCC-3' (SEQ ID NO:3) and R:5'-Fluorescein-CTACAAGCTGGGCTCAGGG-3' (SEQ ID NO:4)) was designed for the amplification of the IGF2 microsatellite, which is about 250 by long and located approximately 800 by downstream from the stop codon. The microsatellite was PCR amplified using fluorescently labeled primers and the genotyping was carried out using an ABI377 sequencer and the GeneScan/Genotyper software (Perkin Elmer, USA). Two-point and multipoint linkage analysis were done with the Cri-Map software.²⁶

[0068]Animals and Phenotypic Data.

[0069]The intercross pedigree comprised two European Wild Boar males and eight Large White females, 4 F₁ males and 22 F₁ females, and 200 F₂ progeny.¹ The F₂ animals were sacrificed at a live weight of at least 80 kg or at a maximum age of 190 days. Phenotypic data on birth weight, growth, fat deposition, body composition, weight of internal organs, and meat quality were collected; a detailed description of the phenotypic traits are provided by Andersson et al.¹ and Andersson-Eklund et al.⁴

[0070]Statistical Analysis.

[0071]Interval mapping for the presence of QTL were carried out with a least squares method developed for the analysis of crosses between outbred lines.²⁷ The method is based on the assumption that the two divergent lines are fixed for alternative QTL alleles. There are four possible genotypes in the F₂ generation as regards the grandparental origin of the alleles at each locus. This makes it possible to fit three effects: additive, dominance, and imprinting.² The latter is estimated as the difference between the two types of heterozygotes, the one receiving the Wild Boar allele through an F₁ sire and the one receiving it from an F₁ dam. An F-ratio was calculated using this model (with 3 d.f.) versus a reduced model without a QTL effect for each cM of chromosome 2. The most likely position of a QTL was obtained as the location giving the highest F-ratio. Genome-wise significance thresholds were obtained empirically by a permutation test²⁸ as described.² The QTL model including an imprinting effect was compared with a model without imprinting (with 1 d.f.) to test whether the imprinting effect was significant.

[0072]The statistical models also included the fixed effects and covariates that were relevant for the respective traits; see Andersson-Eklund et al.⁴ for a more detailed description of the statistical models used. Family was included to account for background genetic effects and maternal effects. Carcass weight was included as a covariate to discern QTL effects on correlated traits, which means that all results concerning body composition were compared at equal weights. Least-squares means for each genotype class at the IGF2 locus were estimated with a single point analysis using Procedure GLM of SAS;²⁹ the model included the same fixed effects and covariates as used in the interval mapping analyses.

[0073]The QTL shows a clear parent of origin-specific expression and the map position coincides with that of the insulin-like growth factor II gene (IGF2), indicating IGF2 as the causative gene. A highly significant segregation distortion (excess of Wild Boar-derived alleles) was also observed at this locus. The results demonstrate an important effect of the IGF2 region on postnatal development and it is possible that the presence of a paternally expressed IGF2-linked QTL in humans and in rodent model organisms has so far been overlooked due to experimental design or statistical treatment of data. The study also has important implications for quantitative genetics theory and practical pig breeding.

[0074]IGF2 was identified as a positional candidate gene for this QTL due to the observed similarity between pig chromosome 2p and human chromosome 11p. A genomic IGF2 clone was isolated by screening a porcine BAC library. FISH analysis with this BAC clone gave a strong consistent signal on the terminal part of chromosome 2p (FIG. 1). A polymorphic microsatellite is located in the 3'UTR of IGF2 in mice (GenBank U71085), humans (GenBank S62623), and horse (GenBank AF020598). The possible presence of a corresponding porcine microsatellite was investigated by direct sequencing of the IGF2 3'UTR using the BAC clone. A complex microsatellite was identified about 800 by downstream of the stop codon; a sequence comparison revealed that this microsatellite is identical to a previously described anonymous microsatellite, Swc9.⁶ PCR primers were designed and the microsatellite (IGF2ms) was found to be highly polymorphic with three different alleles among the two Wild Boar founders and another two among the eight Large White founders. IGF2ms was fully informative in the intercross as the breed of origin as well as the parent of origin could be determined with confidence for each allele in each F₂ animal.

[0075]A linkage analysis using the intercross pedigree was carried out with IGF2ms and the microsatellites Sw2443, Sw2623, and Swr2516, all from the distal end of 2p.⁷ IGF2 was firmly assigned to 2p by highly significant lod scores (e.g., Z=89.0, 0=0.003 against Swr2516). Multipoint analyses, including previously typed chromosome 2 markers,⁸ revealed the following order of loci (sex-average map distances in Kosambi cM): Sw2443/Swr2516-0.3-IGF2-14.9-Sw2623-10.3-Sw256. No recombinant was observed between Sw2443 and Swr2516, and the suggested proximal location of IGF2 in relation to these loci is based on a single recombinant giving a lod score support of 0.8 for the reported order. The most distal marker in our previous QTL study, Sw256, is located about 25 cM from the distal end of the linkage group.

[0076]QTL analyses of body composition, fatness, meat quality, and growth traits were carried out with the new chromosome 2 map using a statistical model testing for the possible presence of an imprinting effect as expected for IGF2. Clear evidence for a paternally expressed QTL located at the very distal tip of 2p was obtained (FIG. 2; Table 1). The QTL had very large effects on lean meat content in ham and explained an astonishing 30% of the residual phenotypic variance in the F₂ population. Large effects on the area of the longissumus dorsi muscle, on the weight of the heart, and on back-fat thickness (subcutaneous fat) were also noted. A moderate effect on one meat quality trait, reflectance value, was indicated. The QTL had no significant effect on abdominal fat, birth weight, growth, weight of liver, kidney, or spleen (data not shown). The Large White allele at this QTL was associated with larger muscle mass and reduced back-fat thickness consistent with the difference between this breed and the Wild Boar population. The strong imprinting effect observed for all affected traits strongly suggests a single causative locus. The pleiotropic effects on skeletal muscle mass and the size of the heart appear adaptive from a physiological point of view as a larger muscle mass requires a larger cardiac output. The clear paternal expression of this QTL is illustrated by the least squares means which fall into two classes following the population origin of the paternally inherited allele (Table 1). It is worth noticing though that there was a non-significant trend towards less extreme values for the two heterozygous classes, in particular for the estimated effect on the area of longissimus dorsi. This may be due to chance, but could have a biological explanation, e.g., that there is some expression of the maternally inherited allele or that there is a linked, non-imprinted QTL with minor effects on the traits in question.

[0077]The IGF2-linked QTL and the FAT1 QTL on chromosome 4¹, 9 are by far the two loci with the largest effect on body composition and fatness segregating in this Wild Boar intercross. The IGF2 QTL controls primarily muscle mass whereas FAT1 has major effects on fat deposition including abdominal fat, a trait that was not affected by the IGF2 QTL (FIG. 2). No significant interaction between the two loci was indicated and they control a very large proportion of the residual phenotypic variance in the F₂ generation. A model including both QTLs explains 33.1% of the variance for percentage lean meat in ham, 31.3% for the percentage of lean meat plus bone in back, and 26.2% for average back fat depth (compare with a model including only chromosome 2 effects, Table 1). The two QTLs must have played a major role in the response during selection for lean growth and muscle mass in the Large White domestic pig.

[0078]A highly significant segregation distortion was observed in the IGF2 region (excess of Wild Boar-derived alleles) as shown in Table 1 (χ=11.7, d.f.=2; P=0.003). The frequency of Wild Boar-derived IGF2 alleles was 59% in contrast to the expected 50% and there was twice as many "Wild Boar" as "Large White" homozygotes. This deviation was observed with all three loci at the distal tip and is thus not due to typing errors. The effect was also observed with other loci but the degree of distortion decreased as a function of the distance to the distal tip of the chromosome. Blood samples for DNA preparation were collected at 12 weeks of age and we are convinced that the deviation from expected Mendelian ratios was present at birth as the number of animals lost prior to blood sampling was not sufficient to cause a deviation of this magnitude. No other of the more than 250 loci analyzed in this pedigree show such a marked segregation distortion (L. Andersson, unpublished). The segregation distortion did not show an imprinting effect, as the frequencies of the two reciprocal types of heterozygotes were identical (Table 1). This does not exclude the possibility that the QTL effects and the segregation distortion are controlled by the same locus. The segregation distortion may be due to meiotic drive favoring the paternally expressed allele during gametogenesis, as the F₁ parents were all sired by Wild Boar males. Another possibility is that the segregation distortion may be due to codominant expression of the maternal and paternal allele in some tissues and/or during a critical period of embryo development. Biallelic IGF2 expression has been reported to occur to some extent during human development¹⁰, 11 and interestingly a strong influence of the parental species background on IGF2 expression was recently found in a cross between Mus musculus and Mus spretus.¹² It is also interesting that a VNTR polymorphism at the insulin gene, which is very closely linked to IGF2, is associated with size at birth in humans.¹³ It is possible that the IGF2-linked QTL in pigs has a minor effect on birth weight but in our data it was far from significant (FIG. 2) and there was no indication of an imprinting effect.

[0079]This study is an advance in the general knowledge concerning the biological importance of the IGF2 locus. The important role of IGF2 for prenatal development is well-documented from knock-out mice,¹⁴ as well as from its causative role in the human Beckwith-Wiedemann syndrome.¹⁵ This study demonstrates an important role for the IGF2-region also for postnatal development. It should be stressed that our intercross between outbred populations is particularly powerful to detect QTL with a parent of origin-specific effect on a multifactorial trait. This is because multiple alleles (or haplotypes) are segregating and we could deduce whether a heterozygous F₂ animal received the Wild Boar allele from the F₁ male or female. It is quite possible that the segregation of a paternally expressed IGF2-linked QTL affecting a trait like obesity has been Overlooked in human studies or in intercrosses between inbred rodent populations because of experimental design or statistical treatment of data. An imprinting effect cannot be detected in an intercross between two inbred lines as only two alleles are segregating at each locus. Our result has therefore significant bearings on the future analysis of the association between genetic polymorphism in the insulin-IGF2 region and Type I diabetes,¹⁶ obesity,¹⁷ and variation in birth weight¹³ in humans, as well as for the genetic dissection of complex traits using inbred rodent models. A major impetus for generating an intercross between the domestic pig and its wild ancestor was to explore the possibilities to map and identify major loci that have responded to selection. We have now showed that two single QTLs on chromosome 2 (this study) and 4¹, 2 explain as much as one third of the phenotypic variance for lean meat content in the F₂ generation. This is a gross deviation from the underlying assumption in the classical infinitesimal model in quantitative genetics theory namely that quantitative traits are controlled by an infinite number of loci each with an infinitesimal effect. If a large proportion of the genetic difference between two divergent populations (e.g., Wild Boar and Large White) is controlled by a few loci, one would assume that selection would quickly fix QTL alleles with large effects leading to a selection plateau. However, this is not the experience in animal breeding programs or selection experiments where good persistent long-term selection responses are generally obtained, provided that the effective population size is reasonably large.¹⁸ A possible explanation for this paradox is that QTL alleles controlling a large proportion of genetic differences between two populations may be due to several consecutive mutations; this may be mutations in the same gene or at several closely linked genes affecting the same trait. It has been argued that new mutations contribute substantially to long-term selection responses,¹⁹ but the genomic distribution of such mutations is unknown.

[0080]The search for a single causative mutation is the paradigm with regard to the analysis of genetic defects in mice and monogenic disorders in humans. We propose that this may not be the case for loci that have been under selection for a large number of generations in domestic animals, crops, or natural populations. This hypothesis predicts the presence of multiple alleles at major QTL. It gains some support from our recent characterization of porcine coat color variation. We have found that both the alleles for dominant white color and for black-spotting differ from the corresponding wild type alleles by at least two consecutive mutations with phenotypic effects at the KIT and MC1R loci, respectively.²⁰, 21 In this context it is highly interesting that in the accompanying example we have identified a third allele at the IGF2-linked QTL. The effects on muscle mass of the three alleles rank in the same order as the breeds in which they are found i.e., Pietrain pigs are more muscular than Large White pigs that in turn have higher lean meat content than Wild Boars.

[0081]There are good reasons to decide that IGF2 is the causative gene for the now reported QTL. Firstly, there is a perfect agreement in map localization (FIG. 2). Secondly, it has been shown that IGF2 is paternally expressed in mice, humans, and now in pigs, like the QTL. There are several other imprinted genes in the near vicinity of IGF2 in mice and humans (Mash2, INS2, H19, KVLQT1, TAPA1/CD81, and CDKN1C/p57.sup.KIP2) but only IGF2 is paternally expressed in adult tissues.²² We believe that this locus provides a unique opportunity for molecular characterization of a QTL. The clear paternal expression can be used to exclude genes that do not show this mode of inheritance. Moreover, the presence of an allelic series should facilitate the difficult distinction between causative mutations and linked neutral polymorphism. We have already shown that there is no difference in coding sequence between IGF2 alleles from Pietrain and Large White pigs suggesting that the causative mutations occur in regulatory sequences. An obvious step is to sequence the entire IGF2 gene and its multiple promoters from the three populations. The recent report that a VNTR polymorphism in the promoter region of the insulin (INS) gene affects IGF2 expression²³ suggests that the causative mutations may be at a considerable distance from the IGF2 coding sequence.

[0082]The results have several important implications for the pig breeding industry. They show that genetic imprinting is not an esoteric academic question but need to be considered in practical breeding programs. The detection of three different alleles in Wild Boar, Large White, and Pietrain populations indicates that further alleles at the IGF2-linked QTL segregate within commercial populations. The paternal expression of the QTL facilitates its detection using large paternal half-sib families as the female contribution can be ignored. The QTL is exploited to improve lean meat content by marker-assisted selection within populations or by marker-assisted introgression of favorable alleles from one population to another.

Example 2

Pietrain x Large White Intercrosses

[0083]Methods

[0084]Pedigree material: The pedigree material utilized to map QTL was selected from a previously described Pietrain x Large White F2 pedigree comprising >1,800 individuals.⁶, 7 To assemble this F2 material, 27 Pietrain boars were mated to 20 Large White sows to generate an F1 generation comprising 456 individuals. 31 F1 boars were mated to unrelated 82 F1 sows from 1984 to 1989, yielding a total of 1862 F2 offspring. F1 boars were mated on average to 7 females, and F1 sows to an average of 2.7 males. Average offspring per boar were 60 and per sow 23.

[0085]Phenotypic information: (i) Data collection: A total of 21 distinct phenotypes were recorded in the F2 generation.⁶, 7 These included: [0086]five growth traits: birth weight (g), weaning weight (Kg), grower weight (Kg), finisher weight (Kg) and average daily gain (ADG; Kg/day; grower to finisher period); [0087]two body proportion measurements: carcass length (cm); and a conformation score (0 to 10 scale; reference 6); [0088]ten measurements of carcass composition obtained by dissection of the chilled carcasses 24 hours after slaughter. These include measurements of muscularity: % ham (weight hams/carcass weight), % loin (weight loin/carcass weight), % shoulder (weight shoulder/carcass weight), % lean cuts (% ham+% loin+% shoulder); and measurements of fatness: average back-fat thickness (BFT; cm), % back fat (weight back fat/carcass weight), % belly (weight belly/carcass weight), % leaf fat (weight leaf fat/carcass weight), % jowl (weight jowl/carcass weight), and "% fat cuts" (% back fat+% belly+% leaf fat+% jowl). [0089]four meat quality measurements: pH_LD1 (Longissimus dorsi 1 hour after slaughter), pH_LD24 (Longissimus dorsi 24 hours after slaughter), pH_G1 (Gracilis 1 hour after slaughter) and pH_G24 (Gracilis 24 hours after slaughter). (ii) Data processing: Individual phenotypes were preadjusted for fixed effects (sire, dam, CRC genotype, sex, year-season, parity) and covariates (litter size, birth weight, weaning weight, grower weight, finisher weight) that proved to significantly affect the corresponding trait. Variables included in the model were selected by stepwise regression.

[0090]Marker genotyping: Primer pairs utilized for PCR amplification of microsatellite markers are as described.¹⁹ Marker genotyping was performed as previously described.²⁰ Genotypes at the CRC and MyoD loci were determined using conventional methods as described.¹, 12 The LAR test for the Igf2 SNP was developed according to Baron et al.²¹ using a primer pair for PCR amplification (5'-CCCCTGAACTTGAGGACGAGCAGCC-3' (SEQ ID NO:5); 5'-ATCGCTGTGGGCTGGGTGGGCTGCC-3' (SEQ ID NO:6)) and a set of three primers for the LAR step (5'-FAM-CGCCCCAGCTGCCCCCCAG-3' (SEQ ID NO:7); 5'-HEX-CGCCCCA GCTGCCCCCCAA-3' (SEQ ID NO:8); 5'-CCTGAGCTGCAGCAGGCCAG-3' (SEQ ID NO:9)).

[0091]Map construction: Marker maps were constructed using the TWOPOINT, BUILD and CHROMPIC options of the CRIMAP package.²² To allow utilization of this package, full-sib families related via the boar or sow were disconnected and treated independently. By doing so, some potentially usable information was neglected, yielding, however, unbiased estimates of recombination rates.

[0092]QTL mapping: (i) Mapping Mendelian QTL: Conventional QTL mapping was performed using a multipoint maximum likelihood method. The applied model assumed one segregating QTL per chromosome, and fixation of alternate QTL alleles in the respective parental lines, Pietrain (P) and Large White (LW). A specific analysis program had to be developed to account for the missing genotypes of the parental generation, resulting in the fact that the parental origin of the F1 chromosomes could not be determined. Using a typical "interval mapping" strategy, a hypothetical QTL was moved along the marker map using user-defined steps. At each position, the likelihood (L) of the pedigree data was computed as:

L = Φ = 1 2 r i = 1 n G = 1 4 ( P ( G M i , θ , Φ ) P ( y i G ) ) ##EQU00001##

[0093]where

Φ = 1 2 r : ##EQU00002##

is the sum over all possible marker-QTL phase combinations of the F1 generation. As there are two possible phases for each parent (left chromosome P or right chromosome P), there is a total of 2^r combinations for r F1 parents.

i = 1 n ##EQU00003##

is the product over the n F2

G = 1 4 ##EQU00004##

is the sum, for the ith F2 offspring, over the four possible QTL genotypes: P/P, P/LW, LW/P and LW/LW

[0094]P(G|M_i,θ,φ) is the probability of the considered QTL genotype, given (i) M_i: the marker genotype of the ith F2 offspring and its F1 parents, (ii): the vector of recombination rates between adjacent markers and between the hypothetical QTL and its flanking markers, and (iii) θ the considered marker-QTL phase combination of the F1 parents.

[0095]Recombination rates and marker linkage phase of F1 parents are assumed to be known when computing this probability. Both were determined using CRIMAP in the map construction phase (see above).

[0096]P(y_i|G) is the probability of the phenotypic value of (y_i) of offspring i, given the QTL genotype under consideration. This probability is computed from the normal density function:

P ( y i G ) = 1 2 π σ - ( y i - μ G ) 2 2 σ 2 ##EQU00005##

[0097]where μ_G is the phenotypic mean of the considered QTL genotype (PP, PL, LP or LL) and σ² the residual variance σ² was considered to be the same for the four QTL genotypic classes.

[0098]The values of μ_PP, μ_PL=μ_LP, μ_LL and σ² maximizing L were determined using the GEMINI optimization routine.²³

[0099]The likelihood obtained under this alternative H₁ hypothesis was compared with the likelihood obtained under the null hypothesis H₀ of no QTL, in which the phenotypic means of the four QTL genotypic classes were forced to be identical. The difference between the logarithms of the corresponding likelihoods yields a lodscore measuring the evidence in favor of a QTL at the corresponding map position.

[0100](ii) Significance thresholds: Following Lander & Botstein,²⁴ lodscore thresholds (T) associated with a chosen genome-wise significance level, were computed such that:

α=(C+9.21GT)χ₂²(4.6 T)

[0101]where C corresponds to the number of chromosomes (=19), G corresponds to the length of the genome in Morgans (=29), and χ₂²(4.6 T) denotes one minus the cumulative distribution function of the chi-squared distribution with 2 d.f. Single point 2 ln(LR) were assumed to be distributed as a chi-squared distribution with two degrees of freedom, as we were fitting both an additive and dominance component. To account for the fact that we were analyzing multiple traits, significance levels were adjusted by applying a Bonferoni correction corresponding to the effective number of independent traits that were analyzed. This effective number was estimated at 16 following the approach described by Spelman et al.²⁵ Altogether, this allowed us to set the lodscore threshold associated with an experiment-wise significance level of 5% at 5.8. When attempting to confirm the identified QTL in an independent sample, the same approach was used, however, setting C at 1, G at 25 cM and correcting for the analysis of 4.5 independent traits (as only six traits were analyzed in this sample). This yielded a lodscore threshold associated with a Type I error of 5% of 2.

[0102](iii). Testing for an imprinted QTL: To test for an imprinted QTL, we assumed that only the QTL alleles transmitted by the parent of a given sex would have an effect on phenotype, the QTL alleles transmitted by the other parent being "neutral." The likelihood of the pedigree data under this hypothesis was computed using equation 1. To compute P(y_i|G), however, the phenotypic means of the four QTL genotypes were set at μ_PP=μ_PL=μ_P and μ_LP=μ_LL=μ_L to test for a QTL for which the paternal allele only is expressed, and μ_PP=μ_LP=μ_P and μ_PL=μ_LL=μ_L to test for a QTL for which the maternal allele only is expressed. It is assumed in this notation that the first subscript refers to the paternal allele, the second subscript to the maternal allele. H₀ was defined as the null-hypothesis of no QTL, H₁ testing the presence of a Mendelian QTL; H₂ testing the presence of a paternally expressed QTL, and H₃ testing the presence of a maternally expressed QTL.

[0103]RT-PCR: Total RNA was extracted from skeletal muscle according to Chirgwin et al.²⁶ RT-PCR was performed using the Gene-Amp RNA PCR Kit (Perkin-Elmer) The PCR products were purified using QiaQuick PCR Purification kit (Qiagen) and sequenced using Dye terminator Cycle Sequencing Ready Reaction (Perkin Elmer) and an AB1373 automatic sequencer.

[0104]In Example 2, we report the identification of a QTL with major effect on muscle mass and fat deposition mapping to porcine 2p1.7 The QTL shows clear evidence for parental imprinting strongly suggesting the involvement of the Igf2 locus.

[0105]A Pietrain X Large White intercross comprising 1125 F₂ offspring was generated as described.⁶, 7 The Large White and Pietrain parental breeds differ for a number of economically important phenotypes. Pietrains are famed for their exceptional muscularity and leanness⁸ (FIG. 2), while Large Whites show superior growth performance. Twenty-one distinct phenotypes measuring (i) growth performance (5), (ii) muscularity (6), (iii) fat deposition (6), and (iv) meat quality (4), were recorded on all F₂ offspring.

[0106]In order to map QTL underlying the genetic differences between these breeds, we undertook a whole genome scan using microsatellite markers on an initial sample of 677 F₂ individuals. Analysis of pig chromosome 2 using a ML multipoint algorithm, revealed highly significant lodscores (up to 20) for six of the 12 phenotypes measuring muscularity and fat deposition at the distal end of the short arm of chromosome 2 (FIG. 3A). Positive lodscores were obtained for the remaining six phenotypes, however, not reaching the genome-wise significance threshold (=5%). To confirm this finding, the remaining sample of 355 F₂ offspring was genotyped for the five most distal 2p markers and QTL analysis performed for the traits yielding the highest lodscores in the first analysis. Lodscores ranged from 2.1 to 7.7, clearly confirming the presence of a major QTL in this region. Table 2 reports the corresponding ML estimates for the three genotypic means as well as the corresponding residual variance.

[0107]Bidirectional chromosome painting establishes a correspondence between SSC2p and HSA11pter-q13.⁹, 10 At least two serious candidate genes map to this region in man: the myogenic basic helix-loop-helix factor, MyoD, maps to HSA11p15.4, while Igf2 maps to HSA11p15.5 MyoD is a well known key regulator of myogenesis and is one of the first myogenic markers to be switched on during development.¹¹ A previously described amplified sequence polymorphism in the porcine MyoD gene¹² proved to segregate in our F₂ material, which was entirely genotyped for this marker. Linkage analysis positioned the MyoD gene in the SW240-SW776 (odds >1000) interval, therefore well outside the lod-2 drop off support interval for the QTL (FIG. 1). Igf2 is known to enhance both proliferation and differentiation of myoblasts in vitro¹³ and to cause a muscular hypertrophy when overexpressed in vivo. Based on a published porcine adult liver cDNA sequence,¹⁴ we designed primer pairs allowing us to amplify the entire Igf2 coding sequence with 222 by of leader and 280 by of trailer sequence from adult skeletal muscle cDNA. Pietrain and Large White RT-PCR products were sequenced indicating that the coding sequences were identical in both breeds and with the published sequence. However, a G A transition was found in the leader sequence corresponding to exon 2 in man (FIG. 4). We developed a screening test for this single nucleotide polymorphism (SNP) based on the ligation amplification reaction (LAR), allowing us to genotype our pedigree material. Based on these data, Igf2 was shown to colocalize with the SWC9 microsatellite marker (=0%), therefore located at approximately 2 centimorgan from the most likely position of the QTL and well within the 95% support interval for the QTL (FIG. 1). Subsequent sequence analysis demonstrated that the microsatellite marker SWC9 is actually located within the 3' UTR of the Igf2 gene. Combined with available comparative mapping data for the PGA and FSH loci, these results suggest the occurrence of an interstitial inversion of a chromosome segment containing MyoD, but not Igf2 which has remained telomeric in both species.

[0108]Igf2 therefore appeared as a strong positional allele having the observed QTL effect. In man and mouse, Igf2 is known to be imprinted and to be expressed exclusively from the paternal allele in several tissues.¹⁵ Analysis of skeletal muscle cDNA from pigs heterozygous for the SNP and/or SWC9, shows that the same imprinting holds in this tissue in the pig as well (FIG. 4). Therefore if Igf2 were responsible for the observed effect, and knowing that only the paternal Igf2 allele is expressed, one can predict that (i) the paternal allele transmitted by F1 boars (P or LW) would have an effect on phenotype of F2 offspring, (ii) the maternal allele transmitted by F1 sows (P or LW) would have no effect on phenotype of F2 offspring, and (iii) the likelihood of the data would be superior under a model of a bimodal (1:1) F2 population sorted by inherited paternal allele when compared to a conventional "Mendelian" model of a trimodal (1:2:1) F2 population. The QTL mapping programs were adapted in order to allow testing of the corresponding hypotheses. H₀ was defined as the null-hypothesis of no QTL, H₁ as testing for the presence of a Mendelian QTL, H₂ as testing for the presence of a paternally expressed QTL, and H₃ as testing for the presence of a maternally expressed QTL.

[0109]FIG. 3 summarizes the obtained results. FIGS. 3A, 3B and 3C, respectively, show the lodscore curves corresponding to log₁₀ (H₂/H₀), log₁₀ (H₃/H₀) and log₁₀ (H₂/H₁). It can be seen that very significant lodscores are obtained when testing for the presence of a paternally expressed QTL, while there is no evidence at all for the segregation of a QTL when studying the chromosomes transmitted by the sows. Also, the hypothesis of a paternally expressed QTL is significantly more likely (log₁₀(H₂/H₁)>3) than the hypothesis of a "Mendelian" QTL for all examined traits. The fact that the same tendency is observed for all traits indicates that it is likely the same imprinted gene that is responsible for the effects observed on the different traits. Table 2 reports the ML phenotypic means for the F2 offspring sorted by inherited paternal QTL allele. Note that when performing the analysis under a model of a Mendelian QTL, the Pietrain and Large White QTL alleles appeared to behave in an additive fashion, the heterozygous genotype exhibiting a phenotypic mean corresponding exactly to the midpoint between the two homzygous genotypes. This is exactly what one would predict when dealing with an imprinted QTL as half of the heterozygous offspring are expected to have inherited the P allele from their sire, the other half inheriting the LW allele.

[0110]These data therefore confirmed our hypothesis of the involvement of an imprinted gene expressed exclusively from the paternal allele. The fact that the identified chromosomal segment coincides precisely with an imprinted domain documented in man and mice strongly implicates the orthologous region in pigs. At least seven imprinted genes mapping to this domain have been documented (Igf2, Ins2, H19, Mash2, p57.sup.KIP2, K_vLQTL1 and TDAG51) (reference 15 and Andrew Feinberg, personal communication). Amongst these, only Igf2 and Ins2 are paternally expressed. While we cannot exclude that the observed QTL effect is due to an as of yet unidentified imprinted gene in this region, its reported effects on myogenesis in vitro and in vivo¹³ strongly implicate Igf2. Particularly the muscular hypertrophy observed in transgenic mice overexpressing Igf2 from a muscle specific promoter is in support of this hypothesis (Nadia Rosenthal, personal communication. Note that allelic variants of the INS VNTR have recently been shown to be associated with size at birth in man,¹⁶ and that the same VNTR has been shown to affect the level of Igf2 expression.¹⁷

[0111]The observation of the same QTL effect in a Large White x Wild Boar intercross indicates the existence of a series of at least three distinct functional alleles. Moreover, preliminary evidence based on marker-assisted segregation analysis points towards residual segregation at this locus within the Pietrain population (data not shown). The occurrence of an allelic series might be invaluable in identifying the causal polymorphisms which--based on the quantitative nature of the observed effect--are unlikely to be gross gene alterations but rather subtle regulatory mutations.

[0112]The effects of the identified QTL on muscle mass and fat deposition are truly major, being of the same magnitude of those reported for the CRC locus⁶, 7 though apparently without the associated deleterious effects on meat quality. We estimate that both loci jointly explain close to 50% of the Pietrain versus Large White breed difference for muscularity and leanness. Understanding the parent-of-origin effect characterizing this locus will allow for its optimal use in breeding programs. Indeed, today half of the offspring from commercially popular Pietrain x Large White crossbred boars inherit the unfavorable Large White allele causing considerable loss.

[0113]The QTL described in this work is the second example of a gene affecting muscle development in livestock species that exhibits a non-Mendelian inheritance pattern. Indeed, we have previously shown that the callipyge locus (related to the qualitative trait wherein muscles are doubled) is characterized by polar overdominance in which only the heterozygous individuals that inherit the CLPG mutation from their sire express the double-muscling phenotype.⁵ This demonstrates that parent-of-origin effects affecting genes underlying production traits in livestock might be relatively common.

Example 3

Generating a Reference Sequence of IGF2 and Flanking Loci in the Pig

[0114]Provided is an imprinted QTL with major effect on muscle mass mapping to the IGF2 locus in the pig, and use of the QTL as tool in marker-assisted selection. To fine tune this tool for marker-assisted selection, as well as to further identify a causal mutation, we have further generated a reference sequence encompassing the entire porcine IGF2 sequence as well as that from flanking genes.

[0115]To achieve this, we screened a porcine BAC library with IGF2 probes and identified two BACs. BAC-PIGF2-1 proved to contain the INS and IGF2 genes, while BAC-PIGF2-2 proved to contain the IGF2 and H19 genes. The NotI map as well as the relative position of the two BACs is shown in FIG. 5. BAC-PIGF2-1 was shotgun sequenced using standard procedures and automatic sequencers. The resulting sequences were assembled using standard software yielding a total of 115 contigs. The corresponding sequences are reported in FIG. 6. Similarity searches were performed between the porcine contigs and the orthologous sequences in human. Significant homologies were detected for 18 contigs and are reported in FIG. 7.

[0116]For BAC-PIGF2-2, the 24 Kb NotI fragment not present in BAC-PIGF2-1 was subcloned and sequenced using the EZ::TN transposon approach and ABI automatic sequencers. Resulting sequences were assembled using the Phred-Phrap-Consed program suit, yielding seven distinct contigs (FIG. 8). The contig sequences were aligned with the corresponding orthologous human sequences using the compare and dotplot programs of the GCG suite. FIG. 9 summarizes the corresponding results.

Example 4

Identification of DNA Sequence Polymorphisms in the IGF2 and Flanking Loci

[0117]Based on the reference sequence obtained as described in Example 1, we resequenced part of the IGF2 and flanking loci from genomic DNA isolated from Pietrain, Large White and Wild Boar individuals, allowing identification of DNA sequence polymorphisms such as reported in FIG. 10.

Example 5

Effect of the IGF2-Intron3-G3072A Mutation on Prolificacy in Sows

[0118]This example shows presently described unique inheritance method of paternal imprinting, wherein only the gene inherited from the father is expressed, and wherein the gene inherited from the mother is a silent gene and has no effect on the carcass quality of the offspring.

[0119]Material and Methods

[0120]Animals. The animals used in this experiment are purebred animals belonging to three different closed dam lines based on Large White and Landrace breeds. From 1999 until 2005 blood samples were collected from all nucleus sows and boars. Genotypic frequencies per line were calculated on 555 sows in total.

[0121]Measurements. Individual blood samples are linked to individual phenotypes. For all sows the following parameters were recorded: total born, live born, stillborn and weaned piglets per litter. At test weight of 110 kg carcass measures were performed on live animals using Piglog 105 (including back fat 1, back fat 2 (3rd-4th rib), loin eye depth and lean meat percentage).

[0122]Genotyping. DNA was extracted from the pig blood samples using the Wizard Genomic DNA purification kit according to procedures provided by the manufacturer (Promega, Madison Wis., US). An allelic discrimination assay was performed using the ABI Prism 7700 sequence detection system (Applied Biosystems). The final concentrations used in the 5 μl master mix were: 2.5 μl Taqman Universal PCR Master Mix, NoAmpErase Ung (Applied Biosystems, Foster City, Calif.), 1× Assay Mix, 10 ng DNA and 2,375 μl H₂O (Foster City, Calif.).

[0123]Statistical analyses. The statistical analysis was performed using the statistical software SAS. The gene frequencies were calculated from PROC FREQ. IGF2 effects were analyzed using SAS PROC GLM with paternal or maternal allele as class variables and taking into account parity and sire. For the calculation of the effect of the IGF2 mutation on the traits measured, a subset of data was made in which only sows that originate from sires that are heterozygous for the IGF2 mutation were used. Sows that inherited the G allele were compared with those that inherited the A allele. Another subset of data was made in which only sows from heterozygous dams were retained. In this dataset the effect of the maternal allele was analyzed.

[0124]Results and Discussion. Allelic frequencies are presented in Table 3. All three dam lines segregate for the IGF2 mutation, although frequencies differ according to the line.

[0125]A subset of data was made in which only sows derived from heterozygous sires that segregate in the population were retained. A comparison was made between sows that inherited the A or the G allele from their father.

[0126]Sows that inherited the wild type allele from their father had significantly more piglets born alive, total born and weaned, while there was no effect on stillborn piglets (Table 4). If the same dataset was analyzed according to the allele inherited from the mother (maternal allele) no effect on any of these prolificacy data could be observed. A second subset of data was created in which only sows from heterozygous dams were taken into account and grouped according to the maternal allele. Again, no significant effect on prolificacy could be observed, which was expected since the maternal allele is not expressed.

[0127]The parity or average number of cycles per sow was also higher in sows that inherited G from their father as compared to those that received the A allele, which points to a beneficial effect on longevity. This is related to higher litter size, since that is a major criterion for elimination in the selection program.

[0128]The effect of the paternal allele for IGF2 was also analyzed on conformation measures at ca. 110 kg live weight. These data are presented in Table 5.

[0129]Although no significant effects of IGF2 paternal allele on Piglog results could be observed, there is a tendency towards higher muscularity and lower back fat in the sows that inherited the A allele form their father. The fact that this difference is not significant could be due to the low number of animals on the one hand, and the use of a threshold value on back fat in the selection program on the other.

[0130]These results show an influence of the IGF2-intron3 G3072A mutation on prolificacy and longevity in sows. This opens the possibilities to use the same imprinted QTN for different selection in sire and dam lines. Terminal sires should be homozygous for the lean allele to give uniform and lean slaughter pigs, while dam lines can benefit from a selection for the wild type allele since this has a beneficial effect on prolificacy and longevity. Because of the imprinted character of the gene, selection for the fatter allele in sow lines will not influence the carcass quality of the offspring.

[0131]A suitable marker-assisted selection program for the IGF2 mutation may now be represented as depicted in FIG. 11.

REFERENCES

Literature Cited in Example 1

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References Used in Example 2

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TABLE-US-00001 [0186]TABLE 1 Summary of QTL analysis for pig chromosome 2 in a Wild Boar/Large White intercross¹ Least squares means⁵ F ratio² Map Percent of F₂ W^P/W^M W^P/L^M L^P/W^M L^P/L^M Trait QTL Imprinting position³ variance⁴ n = 62 n = 43 n = 43 n = 30 Body composition traits Lean meat in ham, % 24.4*** 19.1*** 0 30.6 63.6^a 64.2^a 66.4^b 67.3^b Lean meat mass in ham, kg 18.1*** 16.8*** 1 24.3 4.69^a 4.72^a 4.94^b 5.02^b Lean meat + bone in back, % 12.2** 9.6** 0 17.4 66.3^a 66.7^a 69.3^b 70.8^b Longissimus muscle area, cm² 10.3** 4.8* 1 15.4 31.9^a 33.0^a 34.5^b 35.2^b Fatness traits Average back fat depth, mm 7.1* 8.7** 0 10.4 27.2^a 27.7^a 25.5^b 24.7^b Weight of internal organs Heart, gram 9.7** 11.4*** 0 14.4 226^a 225^a 238^b 244^b Meat quality traits Reflectance value, EEL 5.7 6.1* 1 8.1 18.6^a 18.4^a 21.8^b 19.7^a *P < 0.05; **P < 0.01; ***P < 0.001 ¹Only the traits for which the QTL peak was in the IGF2 region (0-10 cM) and the test statistic reached the nominal significance threshold of F = 3.9 are included. ²"QTL" is the test statistic for the presence of a QTL under a genetic model with additive, dominance, and imprinting effects (3 d.f.) while "Imprinting" is the test statistic for the presence of an imprinting effect (1 d.f.), both obtained at the position of the QTL peak. Genome-wise, significance thresholds, estimated by permutation, were used for the QTL test while nominal significance thresholds were used for the Imprinting test. ³In cM from the distal end of 2p; IGF2 is located at 0.3 cM. ⁴ The reduction in the residual variance of the F₂ population effected by inclusion of an imprinted QTL at the given position. ⁵Means and standard errors estimated at the IGF2 locus by classifying the genotypes according to the population and parent of origin of each allele. W and L represent alleles derived from the Wild Boar and Large White founders, respectively; superscript P and M represent a paternal and maternal origin, respectively. Figures with different letters (superscript a or b) are significantly different at least at the 5% level, most of them are different at the 1% or 0.1% level.

TABLE-US-00002 TABLE 2 Maximum likelihood phenotypic means for the different F2 genotypes estimated under (i) a model of a Mendelian QTL, and (ii) a model assuming an imprinted QTL. Mendelian QTL Imprinted QTL Traits μ_LW/LW μ_LW/P μ_P/P R μ_PAT/LW μ_PAT/P R BFT (cm) 2.98 2.84 2.64 0.27 2.94 2.70 0.27 % ham 21.10 21.56 22.15 0.83 21.23 21.95 0.83 % loin 24.96 25.53 26.46 0.91 25.12 26.14 0.93 % lean cuts 65.02 65.96 67.60 1.65 65.23 67.05 1.67 % back fat 6.56 6.02 5.33 0.85 6.43 5.56 0.85 % fat cuts 28.92 27.68 26.66 1.46 28.54 26.99 1.49

TABLE-US-00003 TABLE 3 Allele frequencies for the IGF2-intron3 G3072A mutation in sows of dam lines (Number of sows within genotypes is presented in parenthesis) Line AA GA GG A 0.04 (4) 0.28 (25) 0.68 (61) B 0.30 (42) 0.37 (52) 0.33 (46) C 0.80 (259) 0.19 (62) 0.01 (4)

TABLE-US-00004 TABLE 4 Effect of paternal allele inherited from heterozygous sires on prolificacy Trait A G Significance* Number of cycles 240 276 analyzed* Born alive/litter 10.37 ± 0.18 10.90 ± 0.16 0.0075 Total born/litter 11.04 ± 0.19 11.48 ± 0.17 0.0371 Stillborn/litter 0.63 ± 0.07 0.59 ± 0.06 NS Weaned/litter 9.11 ± 0.21 9.92 ± 0.16 0.0134 Parity 2.95 ± 0.12 3.54 ± 0.12 0.0035 *Model taking parity and sire into account, NS = not significant P > 0.05

TABLE-US-00005 TABLE 5 Effect of paternal allele inherited from heterozygous sires on carcass measures at 110 kg live weight (Piglog 105) Trait A G Significance* Number of sows analyzed* 70 64 Back fat 1 (mm) 14.90 ± 0.27 15.08 ± 0.27 NS Back fat 2 (mm) 13.20 ± 0.29 14.14 ± 0.30 NS Loin eye (mm) 56.28 ± 0.45 55.72 ± 0.41 NS % lean meat 57.31 ± 0.26 56.69 ± 0.28 NS *Model taking sire into account. NS = not significant P > 0.05

Sequence CWU 1

183121DNAArtificialPrimer 1ggcaagttct tccgctaatg a 21220DNAArtificialPrimer 2gcaccgcaga attacgacaa 20326DNAArtificialPrimer 3gtttctcctg tacccacacg catccc 26419DNAArtificialPrimer 4ctacaagctg ggctcaggg 19525DNAArtificialPrimer 5cccctgaact tgaggacgag cagcc 25625DNAArtificialPrimer 6atcgctgtgg gctgggtggg ctgcc 25719DNAArtificialPrimer 7cgccccagct gccccccag 19819DNAArtificialPrimer 8cgccccagct gccccccaa 19920DNAArtificialPrimer 9cctgagctgc agcaggccag 2010500DNASus scrofa 10gggtgggcag cttcctccca gaccgcagga ggcccaagtt ccctggccct gcccacccag 60ggccagctga agcaggtcag agacacccgc tcctgtccct cctgtcacct aacccaacag 120gccggggccc agggacacag gccacatggc atctcccccc atgcccctgc cccaaggcgc 180ccagcaggtg aggctggagc agagtctggg tcctgcgggc cagaccgagg gcaggacagc 240tgggcatctg tcctcacagt ccccgcgctt tgtcgggagg cggcagagcc tcatccaaga 300cgcccgcaag gaacgggaga aggcggaggc cgcggctgcc gcgtccgagc ccggggaggc 360cctggaagtg ggggcccttg ccgagcggga cgggaaggcc ctgctgaacc tgctcttcac 420cctgagggcc accaagcccc cctcgctgtt ccggtccctg aaaaaattct aggtgagggg 480gcgggccagg gctccccggg 50011943DNASus scrofa 11tgctcctcac accccgggcg gggctgctct tggggccatc ctccccatgg gcccagcacc 60cactctggcc ttcacacctg ccgtcttctg ggaagtcctc tggttcccaa ggaaagtttc 120tgagctggac aagtgccacc acctggtcac caagttcgat cctgagctgg acctggacca 180cccggtgagc cggtgcctcc cctccccggc cgccatgtct cccatcccca ggggtgtccc 240cacactcagg gccgggactg ggcgtgaacc ccgggttggg acggatgttg gcctgctgtg 300tggctcctgg cggaacagag aggcctggct gggtgccacc cccagggccc ccgccgatga 360cacgggccgc gtctgggctg ggcgggcagg gcggccaggc agggcagcct ccgatggcgt 420ccccggctgt caccagggct tctcggacca gttgtaccgc cagcgcagga agctgattgc 480ccagatcgcc ttccagtaca ggcagtaagt ccctccaggg cctcagcctg ggggcccaga 540cctcagcctg ggcctcacgc cagacctggg ggtggaggga agggaggttg tctttgtcac 600caacgccacc accttcactg tcaccatggt caccgactct gggtccccaa atcacagctg 660aggaaactgg ggcacagagt ggttaagcat cttgctgaag ccacacagct ggcggagcat 720ttggccccgg cccctcctgc ggctcccaca cgtgctccct gaggggcccg ggactgacag 780ctgtcccctc ctcagaggtg accctattcc ccgcgtggag tacacagccg aggagattgc 840cacctggtga ggccctgtga cagcggctgg gaggggcggg agtgggggaa gggacaggaa 900gacctcagaa ttcccgcgtg gaacgtggtg gcctctatca tga 943121500DNASus scrofa 12ggggagggga tgctcagacc cgctctggga agaagagagc ctcagaagaa atcccttccc 60aagggtcacg cggtggagcc caggggcccg ctaggggccg attcccacag ctcgtgctgc 120cacctgctgg cgctcccagg aactgcggag gcggtggggg ccctggatgg gtccggcagt 180gggctcgcag gagacccctg gaggggctgc ggacacccca gctgccactc acaaggtgcc 240caagcggcgg tggcaatggg ctgagcctct ccccccctcc tcctccgcag gacattggcc 300tcgcatccct gggggtctcg gacgaggaaa ttgagaagct gtccacggtg ggtttctccc 360cctgcagggc cctgggttcc agccaggccc tcctgtccaa ggggtgtcgt cctcacgctg 420tgaccgcccg ggagcctgga tcggttctgc ctgggtgggc ggtgcccggg ccacgggcag 480caggggcagc ggtgcgggcc ccagccgtgt ctgagccccc ttgccgcctg tccccaccag 540ctgtactggt tcacggtgga gtttgggctc tgcaaacaga acggcgaggt gaaggcctac 600ggggctgggc tgctgtcctc ctacggggag ctcctggtga ggcctccccc acgcgctggg 660gcctgggtcc ccgggggagg tgacccctgc ggtgccttgt ggattccagc tctcgggagg 720ctggagcgag gggctgccct cctgggggca ccaagaaagc tggtctgcgc ccctctccac 780acacctgtgc ctgggccctg gggggacccc tgctggggga tgtgggtgca cagccagggc 840caccagggag tcaggacacg gggctccctt ccctcgggtc cctgagaccc ctggcctccc 900gccagcactc cctgtccgag gagcccgaga tccgggcctt cgaccccgac gcggcggccg 960tgcagcccta ccaggaccag acctaccagc ccgtctactt cgtgtctgag agtttcagtg 1020acgccaagga caagctcagg tgggccgggg cccggggccc ccaaactgga ggatccagcc 1080tgcagccccg cctatgagcc catttcccag cagagggagc tgctgcggac cccaccgtca 1140caacccccct cccacagctg gaaccccaga aagcctgcgg aggggggacc tgcagggctg 1200tggccaggtc aaggccaggt cgaggccagg cttttagggg tgaagtctga ctttgtaaga 1260gggggtgcag ggtccttccc agcctcctcc cctccgagca gctgggggcg gggcgggggt 1320gcgatgaagg cagagatgac gcagccaccc gttcaccctc aggaggcgcc tcctgtccag 1380ccaggctcct gttgtcacag gggaaactga ggccccaggt gtgtgtgtgg gggggtgatt 1440ctcacacaca agcttaggga cagggacata acggcctctc cagggcacac agtctggagg 1500133024DNASus scrofamisc_feature(5)..(5)n is a, c, g, or t 13ttaantccan gttggcccga caagttttcc ccatttgaaa aggggccagt taagccccaa 60cncaattaat tggaagttag ctcccctcat taggctcccc agnctttacn ctttatgttc 120cggttcgtat ttttgtggga attgtagcgg atacaatttc tctcaagnaa ccagctatgc 180ccatgattac gcggtacagt agttcatcag tcccccccgc ccatgggaca gcgaagggaa 240ccagtatgtc gtggggccgg gtctaaaggg gtcaccacca gggaggggca ggggctccag 300gaggcagggc cactgagcgg tacctggtgg ggggaggtgg tggggccaca cccaggagtc 360ctgtgccccc cccactcccg ccgttggaca tgagaagcag gggccagcct gcgggtccct 420gagttcagcg cccccccccc ccaccgccgc agcagcccgg ggtctcagca ggctgctgtg 480ctgggggcgg gggcgcttat ggrgccggga gcagcccccc cccacggctt cagagcatct 540ctggggcctc agggatggac cggggtctgc rggcaggtgt cctctcgcgc ccccactccc 600tgggctataa cgtggaagat gcggcccaag cccggkcggt ttggcctttg tccccagcca 660gtggggacag cctggccctc aggccgctcg ttaagactct aatgacctca aggcccccag 720aggcgctgat gacccacgga gatgatcccg caggcctggc agcagggaaa tgatccagaa 780agtgccacct cagcccccag ccatctgcca cccacctgga ggccctcagg ggccgggcgc 840cggggggcag gcgctataaa gccggccggg cccagccgcc cccagccctc tgggaccagc 900tgtgttccca ggccaccggc aagcaggtct gtccccctgg gctcccgtca gctgggtctg 960ggctgtcctg ctggggccag ggcatctcgg caggaggacg tgggctcctc tctcggagcc 1020cttggggggt gaggctggtg ggggctgcag gtgcccctgg ctggcctcaa cgccgcccgt 1080cccccaggtc ctcacccccc gccatggccc tgtggacgcg cctcctgccc ctgctggccc 1140tgctggcsct ctgggcgccc gccccggccc aggccttcgt gaaccagcac ctgtgcggct 1200cccacctggt ggaggcgctg tacctggtgt gcggggagcg cggcttcttc tacacgccca 1260aggcccgtcg ggaggcggag aaccctcagg gtgagccgag ggggygtccc gggagcggty 1320gggggagttt ttaaggagga aattggtaaa agtgaccaac tccctgggag ctgagcccag 1380agacacccct cccacgcccy ggtcccgctc gagaagcccc ccttccctcc cctcctcccg 1440aggcggctcc agggaggaat cttacggagt caaggcccgg gtgccgctgg tctccgagtg 1500acatggccgt ggtgtcccrt ctgccggccc acatgcccgt gagagawgcc ccatccccct 1560gggagggggc cccgtgccgg gcaggcggcg ggaggcccag gaccggtggc tgctgcggct 1620tccactccag ggtgggcggg gtggggggtg gctgtctctg tgtgaccggc tctccccgca 1680gcaggtgccg tggagctggg cggaggcctg ggcggcctgc aggccctggc gctggagggg 1740cccccgcaga agcgtggcat cgtggagcag tgctgcacca gcatctgttc cctctaccag 1800ctggagaact actgcaacta ggccgcccct gagggcgcct gctgctcccc gcaccccaaa 1860acccaataaa gtcctgaatg agcccggccg agtcctgtgg tctgtgtggc ctggggcggg 1920ggccctggtg ggggaggggc cagaaggctg tggggggcct gcctgcgacc cctctctgct 1980ctcgccacat cggctgctct aagcttcctc cacatgcatc gggtgcccac aggcacatgg 2040gcaccggggg accagggccc agggcagggc ccttcaatgt ggcgagctct ggttttcagg 2100gctccagaca ccccctcctg ggtgcccact gctgcacagg gtcactctga gggtcacagg 2160gcacccaccc agactgctct tgggcacaca aaatagccca ggggcttctt gggctggctg 2220crgtctggga ggtcagagag tgaccccgcg ggaccaagac ctggccagcc tgccagtcgc 2280ccaggccaaa ccaatctgca cctttgctga aggttccacc cgggccagca ctgggggcgg 2340ccgggcctag agctgggcgc ccgggcccca gggactgcac acccgccaga ggtgggcctg 2400aggggtggca gcaggctctc cgcctgggac ccagccagct gggcagctca cctctcaaca 2460cgaggctctc acctgtgtcg tcccctcccc acggccacac agacacccct ggggagaagt 2520cacaggcccc cagcaggccc cgcccctgga gaggaggcca gggctgggca ggcgggtggc 2580cggccggaca ctggacccgg aaggggggta ggcggctggg atgagtggcg agctgtccat 2640gggagcaccc agcggcccca ttggcaccag tacaggcagg ggcacctgca gcagctgagg 2700tacgtggggt ccccggactg gttggtgtcc ggctgccctc tgggaggcag cgggctgagc 2760ttgtggtcct gccaaccagg gagacccgtg accaccctgc tgcttcccct cccccccagg 2820gccagcagac tcctttggga ctcggggccc ctgagccgcc cccactcgca ggactcacgg 2880ggtgtgcggt cctgggtgag tgggggcttg ggagagggtc actcttgtcc gtcgggtggg 2940gaaggctgag agtcatggtg tgacagcgcc ctcggcctgc cgggtggggg gtctcccttc 3000tcccgagccc agatccccgg gtac 3024141730DNASus scrofamisc_feature(530)..(530)n is a, c, g, or t 14cgtcacccgc agaagccagg cccacaggcc ttggctcagc ccctccaccc aggcccacgt 60tccgcccctt ctgggaactg gaggacagcc cgccctcgcc ctcggacctg gcttcgtttg 120ccctggcatc tggcagtggc cggcagctgc gttcagccct ggatgacacc ctggcgtgag 180cggtgggtcc ccgtgctgag ggcagccccc acacacgtcc tgctcacttg ccttgtgtct 240gctccgcatc ccgtcatcac acatgccatg ctggggcacc gtagcgcctt gccctgtgtg 300gcactgtggc actgtgttcc tgatgggaag actgaggctg gggtcaggcc cgctgctgcc 360caccctctaa ggacattctg ccggtgcagc tgcctccagg ctggcccccc ggattgcatc 420tgcttctggc acggatgaac tggcacctct gcctgaccat tagggctgta tttgccttct 480cctgttggca gtaaatattt actgtccctc cctgttcctc caggcccgan ccagttcctg 540aggggcatgg gaggtggaca caaaggtgcc caagcagccc cctgctcttg agggcccagt 600gtctggtggg ggccagcctg ggaaggagga gcgagactag gaaccagagg cctgtgttcc 660tggaaaaggc cccctggcag agttccggct ggtgtgtgtc cagctaggct gtgagtcttc 720aaactgggga gcccggcccc tggacccagg cagggctgca cccctggtgc cagtgcttca 780ctgggtgggc acctgtcccc accaggcaag gtggtccgag cggtcattca cagacagaac 840cagcagaggg cgccaaagcc ccacttttga caaactcccc ttcgccctga gccgaaagtc 900caggcggcag gtggacctct ctgcagggct ctgccacccc tgctgccgct tgccagcact 960cacaggggct gcggggggtg cccaacaggc cggctaccct gagctctgga ggcgatggag 1020tttaggaggg aacgagggga ctcctggggg tgactttctt cagcgcccac attgcggccc 1080agcaaaccga ggctggagga ggccgggcac ctgtgcccag ctggagcctt tgctgagggt 1140ctccaaggcc tggggaaatt gaggctgggg gctggggggt gtcactgtcg ggccaggagg 1200cccctcgctc tgattggagc cgcctcggcc acttgagcca ggaggctcac atgaggcggg 1260ggctgcaggg acaggaccct cggggcccgg gaggccttgg agggggtcca gctgggccag 1320ggttcgttct ttcccgggtc catgtccacc gccctcccgc tgctgggagg agaggaggtc 1380cagggcagaa agaatgcgtg gggatggggg ggtggtcagg ggtctgggag ctgtggaaac 1440aacaaacaga cagcgaggtc ctggggcgcc cggccccccg ccccctccgg cactgttgtt 1500tctggccggg gtgcagggac agcgaggcag attccttcga aagtggagac tggcgggggg 1560cccctcgggt cctcagctca ccccctgagc tagcccgccc actcggctcc aacctcccgc 1620aggcccctgg cacggtctcc aggagtccac tgaggggtcc ccaaagctgc caccaggagc 1680tgggcctggg tctgtcacca ccccacccca ccctccaagt ctgagatatg 1730154833DNASus scrofamisc_feature(127)..(127)n is a, c, g, or t 15atgtgagctg cacagcatga gccctcggcc ccactgctgt ggccttgcgg acattgaggt 60gtgtgccgcc cagggcgacc acaccctggc ctctcagggt gcccgtacag aggcggctgg 120gtcgtangag gtgcggggct ctggggaccg ctggtgagtt caggacgggg gtcatgccac 180ctcctctctg aaggtttggt gaggtggccc ttctcttatc gtgatgacaa tactgatttc 240tggaagagcc aggtgttttc tgaggctgtg gttgcacttc tccacgtggc cacaaggtgc 300cgggctcggg tcagatttga gaagccctgc gggagcgggt gtcatgcgcc agattcagct 360tgcctcctgc gggtctgggg tcaggacgtg gtccccagca gtctgctcca gagcctgtca 420gtgatgtgtg ggattttacc gctagaacac agtttcctct gattctcaga aaccagcaga 480tgctttagga ggggcgtgca ggtttcacct gtgctgcann gccccctgcc acctggtcgg 540agccncaaga cggcatctaa agatcagttc ctcatcatca gttccgcagt gctggggtgg 600gggcagatga gaacctcagg gctgggcgca gaggtgggga gcccgcctgg accccgacac 660tgcagggggg cctccccctt gtaggaagaa caatgtcgct ttgccaccca gccctctccc 720cagggtgccc cgaactgttg ctcctaagac ctctgggctg tgtgctgtaa ttctataagt 780ggccaccagg tgtcagcagg aggccactta agcatccatg tggcggaaac ctggagctgg 840gggttcctaa gggtccctcg agtgtctcct gaataaatag gcgctgacct gatccccagg 900aagggataac cctctcccag gcctaagagg cagtggggca atgaggttta tgtgtccact 960gtacccccaa attgtctctt ccttccctct accctgtgtc cccaccgtgg acgatacacg 1020gagtgcgagg ctgcgggtca cagccctcac agccccaaag ctgcaggtcc tgcctcaggg 1080gcaccgcagc ttggctggtc ccccttgggt cctccccacc ctgacccgtc ctctgctccc 1140ctccctttgc ttaaatgctc tgcgtttcaa ggttctgatg gaataaaata gccctgcact 1200ggtgtgttcc tctttggggc tgtgccagaa gtgggaattc agaccagggc agagctcaga 1260ttccacatac tgtgttaggg atggcaggtg ccacatttcc aggagtttca ttggtggttt 1320gtaaatgcta cttccgtttc agcccctcag ctgcccacct cctcaattta gggacccccc 1380cctttggcgg gttgcccatg gaaccacatc atctggcgtg gggtgagccc tttatcctcc 1440ctggccccac tgggagggtt tggggaagtc ccagctaaat ttctccgtag ggacctggaa 1500ggagcccttg tgacatctgg gcacagataa gaggtagggg gcacaggccg tgaacacttg 1560aagctgcaga gcccagagca gagccagcag gagcaagtga ctgctcccca ccccaagaac 1620tgtgggctgc gtcacacact ccccactgtg tgccctggac ctgacagggc ctttagcctc 1680cctgcatccc tccccaccca agaacccagt gaggcacccc acttgcccct ccttagtgtt 1740gttatggctc tggggcatct gcattttgtt taggacaccc ccagctagat ttaagtcccc 1800ccaagtgtga ctctttcctc cactgaaaac cctgtcctcc caccaaaggg ccctatccct 1860ttagctgagc caaggaaatt caggaggggc cttgaatgac aaaggaagag ggggagagtt 1920aaaccccaac actggctggc aagctgggtg gggtggacac cccagggtgc aggggtgcag 1980tgaaggtagc ggctggtggc cttctggaaa ctacatgtga ctttgccatt aggtgagtct 2040ttgctttgcc cctgctctat ctgcaggctt atggaagaag tttaaattcc cagggacact 2100tggtctaacc aggcagcgct tgtatctggg cccttcccca gctgctgacc actctgagtc 2160tgcgccttag ttggagtttt ggccaagctc aagaggctgt ggaccccagt catcccaccc 2220aggggtgcct gtgggcagga cgctgctgcc tgccatttgc tgcagtattg tcactgtccg 2280gcaccacaca catggtgcag ggggtggtat caggtgccac tggggaaggg agaaaactcc 2340caggtgagtc ccctgcctct ggaagcaaga tggacatgac cgcactgtgt tgcagctgca 2400ttgggaggcc ccgaagaaag atttttctga tctttctcga accctgcttt tccccatcat 2460gccccgcccc cattttaccc gtgccacgcc cactggtgtg ccggggtgtc aagtgactga 2520caagtgtcaa tctactgagg ccctgcccac tctccacccc cccacatagt cccacctccc 2580agctggcagg gagaacttcc agctaatgcc catgcccaca aatgtctttc tgtcagccta 2640gagctggacc aaatctccac cctgtaacat gctgtgccct ggcgtgggaa ggtgccagag 2700ccagttgccc cagcagcccc agaaccacta agttggcaca aagctaccca aatttggagg 2760ggcttgggga agggcatgga ggggatgagg aggtgagggg caaaactaat ttcagttagc 2820atttgagcag gtgccacgct cagcgtggag aggctctctt gcttctaggg acccattatg 2880atgcacacgc taaaagcgcc cttcaccatc tctccagcct cagctttgtc cccctcctcc 2940tcctcagcgg caacccggct ggagggtctg gccactacag ccagagcgcc ccctactttg 3000gtggcgactg ctactattgg cccaaccagc ggatcaccgg ccaggcagtt tcggcagaga 3060gtctggggca ccagtgactc ccccgtcctc tttatccacc acccaggagc ttcagggact 3120acacagcgac tagagggcag gtaactggtc tgccctccct agggctgccc cctcagagtg 3180tgtgagaaaa gctgcattga gtgtttgggt gcaggtgggc tgggggcttg gggcagccaa 3240caggaacggc gggacctctg cttccagagg accccagatc ctggcaagct tcgactttgg 3300aggggacagg aaagacaggt ggagagggga cacttccctc ttctgtacag acgcccaccc 3360ggagccacag aggcttttgc aaggaaaata ggttttccct cactaatgca gcaggcaaaa 3420tgggaggggc aggggtggag ggtagtgccc ccgcccccag caggagggca cagctgtttc 3480tgcaaatgta aaaaagcagg gtttttctgt gtgagaagtt ccctcttgct gcatgtcccc 3540acccccgcca ccaaagacaa acaggacact gtgcagaggg gccagagccc cgagattttg 3600gagttgtttt tatatgcata tataccattt tgaaagcaaa gcttccctct cccctactcc 3660ctacatgtcc cccttcacca aaaaatccca ccacgtaact ggaaagggga gtgagaagga 3720cgacgaaggg gcactgtccc ctcccgtccc acagcgggac ttaaaacgta cagcttttcg 3780cctccggaca gtgtgccgcc ccctggcccc cgtcacgctc ccctgcccgg ggggctgagt 3840gtggggccag ggcctgtctc caggcatgca ttattttgtg catgaaggtt ttgtcccgcc 3900cacccaggct ggtgttgggg ggaaggggtt cattgctcca aagaagccca tctcccccct 3960cagccacctt cagccgcctt cgcaaggcag agctgtgtcc tctgctgtgt gcctggcccc 4020ctccttgctt ctattcaagg tggaagtgtt ggggggagga gaagagtttt tatattgtgt 4080ctgtgatccc ccgaggcagg gcatttgtgt gcggcccccc agcccccagg cccaggcaga 4140tgggccagcc tgcccgacag aagggtctcc tgctgcttgg ctgcagggaa acccagctct 4200gggtgaaccg tgggcacctt ccttcctcca tgccctgtat ttaaagaagg agagctgggg 4260ggccagaggc acagggaggg gagccacggc cccaggtctg acaagatgac ctgcgggcct 4320ctccacccaa gagtcggggt gggggggcgg atttggtttg aaaagagaac aaataggaac 4380acactcttta ttttccccag gggccgaaga gtcacccctg aacttgagga cgagcagccg 4440gattccagcc cccagcccca gggccccaca tctcctcggg ctcagccgcg cgccccagct 4500gccccccagc ctgagctgca gcaggccagg gctgcccgag accccagccc ccaggtgagc 4560tgctgcagcc tgtggcccag gagatctccg ccggctcaga actgaggcgg gcagcccacc 4620cagcccacag cggtgagtgt ctccagaccc cagggcaggg cccggtgtcc cccggcacag 4680agagctgtgc tgcaggccca gacctcccag gccgttttag ttcccatctc cccttggggg 4740aggggtgggg ctcagagggg ctggggtgca tccgcagagc tggggtgcag ggctccaggt 4800gcctctctcc caggcggctg gcccggaggg ggg 4833162064DNASus scrofamisc_feature(46)..(46)n is a, c, g, or t 16ctggtttcgc actcctccgg ggactgttga agtacccgag agcgcncgcg gagcgccggg 60gcgagcgggg gtggccgccg ggggtgctcc cgggcccccg gaccgagcca gggacgagcc 120tgcccgcggc ggcagccggg ccgcggcttc gcctaggctc acagcgcggg agcgcgtggg 180gcgcggccgc tgccgggagt ccgcctgcct cctcggaggc ggccgaccgg ggagcctggg 240ggaccccgag cgcccgggga gcagcgcccc gacacgcccc gggccgctct cggcttcctc 300ccttccagcc ggcgcccgcg cggccgggct tcggcaccgg ggcgctctca gtggcaggag 360aagcgtgcgc tcccgcgggg tgggggaccc gcaggaaacc cgcaccgcct ggagccgccg 420ccgcgcggcc agcgctcgcg tcccccgggg agggcgccac tgctccgcgc gcgcgtcccc 480cgacgccccg cgcgcttccc cggccggccc gggatcctaa cctctctctc ggtcgcagcc 540ccgcatcccc agggctccag gcccccggcg acttgcccgc tcctcccaat tgcagacacg 600actttttctg ggacctccca aaggacagcc tggctccagg gtcccccaga tacattcacc 660atttctccag atcacaagtg ggtttttcgg gcactaactt ccagagacct caaagcacat 720gagcccctac tggctttccc aggtttccac tagtggcctc ggtccccacc tcactgggga 780ttgtctccca ggctcttcgc ggtgtgatcc cacccattcg cgcccaggtc ccgcagtgcc 840aatccctcct ctagaaaact taaacactga ctcctggtct cggggtgagg ctgcccaatg 900tgcctgactc cccagaaggt ataccagtgt ttttctggca tttgggcacc gttcccccaa 960aacacgtgaa gctcttttcc cgcgtcccca taattttgga cgccaggggc acccaagctt 1020agcgcccctg tttggctccc ccacaccgcg aagccctgct ccctggggtt cacgacagtt 1080tgggacttta tctgccaagt tccacaaact gattggcccc aagctggggt ccctaaattg 1140tacacaaaga accccagccc ccccccccaa ctccagtaca ggaagcgatg gccccaggga 1200ccctcggagt tggaacgtgg cttcctaagc cttcaccaaa attgaggctt tccgcgcatg 1260gcgcgctgat gcccttgctg aatcagaagc actctgccct ctgattcctg ctttccacaa 1320ccctgagagc atgatttctg gtcccccaaa ctcactgagc aaaaatcttt ttgtgggggc

1380tgcaaagata ggaggcattt ctctccggag ctctccaaac tcccttgcct ataatcaagt 1440tccctaaaac ttagacagag cttcccaggc cccagaggca cacagagcca ttattggagc 1500tgcgtttaat gatgacaggg accatgggtc atgcagctcc cccaagtcac aaatgcccca 1560ggtatccttg gctccagcca agcccaaagc aaactcttgc acagatccca tatcttgtta 1620tgtcaagcgc tttgcgtgtc ccagtaaaca aatagtctga gtgttttctc cacctcataa 1680cattcggaat attaaaaaat tccctgggcc cccggagctg acagacaaga atccgggctt 1740cctaaaattc agaactgatt cccaaatccc aggccaacgc cagaccctct cccaatctgg 1800agcccctccg actggacaca ctggactcct aagtattacg cgctgtcctc caggcacccc 1860aaatgcattc aaagtgacgc tttggtcaca gaaaggcact gatttcttgg gctccaaagc 1920agcccatgca cccccgagtc accccaaact tagtcagcat ttcccgggtc tccctccgca 1980ctgcaaactc ccaactgcgg acaccggttc ttcaggaccc accgcctaga cggtcttaat 2040cccttttccc ccagacctag attc 206417371DNASus scrofa 17agattcaaaa actatttttc tggggcctcc aaattgaggt gctgcctgcc agtcctccaa 60aataaactga ggggtttttt gtttgtttgt ttttttgttt gtttgttttt tttttacctt 120ccacgaaaca atccaacttt tttggaccat tgatttatgg gtcccctgac tttatgaccc 180ttgccccaag tccccctaaa tgtaggccat tttccacggg cctcccaaaa tgaaattgcc 240cagatcccgc cgaaaaaaat atccccgggt cctggaaatc ccaggtatta caggcctgcg 300gctgacaccc ctccttgcta ctaaccaggt tccctgaagt ttagagatca ctacctaatg 360aacaaatcca c 371182415DNASus scrofa 18ccaaaactgg ggccctatct tactagggtt ccctaaatgc agacagcgcc cgggaaaata 60ggggcgtttt ttttcctgtt tgccaaaaat aaactaattg aaaccaattt ttagaattaa 120aatctaaaat gaccttgatt ttctgcgttc tccaaatgta cttttcacag cccaggttgc 180ccccagttta gacggtgttg cttgaatctc taaagcaccc tgaggatttt tcccgaggaa 240gccaccacaa ctacggaatt tactgtcctt cggggccaca agcctccagg ccaccaactt 300ggatttctaa accgtggaaa tcagcctcca cttccctccg ccaccccgag ggtctgctca 360gaccccccaa acgtgcccgc tgttcttctc cccccaaatt ttatttagag aatatgcctc 420tctcgggttc tgccaagttt cccgctgaga cttcctcggt catccccaaa tcctcttccc 480cacagtccgg gagcccccac aagcttaccg acccacatgc tggggtcccc caacttaaac 540gcgatcccct gtcccccaga ttcaccgagt gatttccctg gtcctcagac tgggactctt 600ttactggagt ctcgaattta gccattaatc acagttctcc actccgacgc aggctccctt 660gggtccccac gtcggggaca tgggttctct tgcctgcaaa tcaggctgct ctgacttgca 720ttcaggcctt tgggcattgt tccccgcccg ccgcggtctc ggttctcccc ccatcccgcg 780cacgacgggc actgggtctg ggcctcttgg tgtctcctac aagtccccgg agctcctcgg 840acttgggaac tgtctcttgc gttccccaaa tacactcggc ccggcagtgt gtccgccagg 900acgtaggcag agcttctccc gcgtccagga aaacgactgg gcattgcccc cagtttcccc 960caaatttggg cattgtccct gggtcttcca acggactggg cgttgccccc ggacactgcg 1020gactgccccc ggggtctcgc tcaccttcag cgcgtccacc gcccgctgca gagcgctcgc 1080tctccgtctc tcggctccca gcgcgcttgg ggacgcagcc tccgggcctc cagccttgcg 1140gtgagctccc cgtcgcctcg cgtgtcccgg cccggctccc aaacccactc gccgccgtcc 1200cgctggggct ggcactggcc tccggcgact gccggggaca cgggagcgga gcgcgggagc 1260ctgctgcagg ccagcccgtc ggccgggccg cgcgccctga aacgcgcgcg gctttcgttt 1320gctctttgca aaggtcacaa ccgtggggaa aacgcctcgg cggcccccaa gcggggcagg 1380cagggcgttg ggaaggaggg acacgcggga gaggagcacc ccgctggggc ggcgcagcgc 1440ggcgcctcca gccgccgggc ggaggatccc gggaggcgcg cgcggagcgc gggcgaagtg 1500attgatggcg gagcgagggg gccagcggat cgcgggcttc cgccggcggc ggccccttcc 1560cctcggaggg actcgggcgg cccgggtttc tgggggcggg cggggcgcgg gggcttgtgc 1620gtggtctcca cttggtaaaa atcacaacga ctttttacgt cgccccgact ctccaggaga 1680tggtttcccc agacccccaa attatcgtgg tggcccccgg ggctgaaccc gcgtctacgc 1740aaggccaacg cgctgaggac gggggaacca ttatccggat attttgggtg ggcccccaaa 1800gcgagctgct tagacgcgcc ccggtgagct cggtcctgca ggtaggcttg gagcgaggtt 1860ccccgccctg ctcctctctc ttcgggcagg cgcggccagg ccggccggcc ctccccacgt 1920acggcacctg gcggccgccg agacgactcc ccggttcccg cgcggcaccg gggggcgctc 1980gggctctggc tgcggctcga ggcgctgcgc ctgctcgggc aggtggaggc ttcacgccgg 2040gcccgcgccc agggacgacc ccttaccccg caggtcccag cgggactcgg ggcccccgga 2100tccagcgtct agccacctgt gcccgcaccg ccgcgagggc ttgtgacacc taccaccctg 2160gccgccccgc gtccccccgc gcacgaatgt agggatcctg acaccccgga acctaagacg 2220gggcccccat acactttcgt acagcgattc gggatttctc tcgaactctg cagatctgta 2280tggcaaagtt gatggcctgc attatttttc tgataattca gcgaaagatg gcgaccagag 2340ctatgcgcgt ctgggtttta aaggcgaaac ccaaattaac gatctggtca acgaacagat 2400acagcatacg ttttt 2415193122DNASus scrofa 19agattccaat ggggatcccg atgaggaagc cgctgctcgt gctgctcgtc ttcttggcct 60tggcctcgtg ctgctatgct gcttaccgcc ccagtgagac tctgtgcggc ggggagctgg 120tggacaccct ccagtttgtc tgcggggacc gcggcttcta cttcagtaag tagctcagcg 180gggcacgggg gcggggcgga cacagcaggt gctccatcgg tgctgccccg gtacctgtgc 240gggtccttcg ggatggatgg tgtgggggac ggggggcggg gggcggccaa gggaggacct 300ctcctccgag ggtctgagac ttcagagcgg gggcgccctg gccctgcgca gtgattggca 360cctgccatgt gcctggctgg ggctcacacc ccctgacgtt cctgcagcgt gactcgaaac 420gggaaaccga agggacgggt ggcacggggt ggggaggcag accgtgagtg gcaggcgtgc 480gaggggttct ttcgggcggg gtggcccagg caggccccac aggatgacag cctgtcccct 540cctgctcctc cttgacctgc ccacagccag ggctgcaggc actgacattc acccatggta 600ttgtggtgcc tgacgtcttg gcagtgggca tgggttcatg gactgttgga ttgaaagtgg 660aataagatgg gttgaaaacc aataagaata aaggcgcgtg tggctggcgg catctgcgag 720aggtgaccgc tgccctccct ggggttgggc tttgggtggg ttcccatggg tggggcgggc 780cgccatgcag ggtgcccgcc tgctggcctc agagtgcttt gccgtcctca tctttctctc 840tggcccccgt cccgctcctg aggctggctg gctgggcccg cggagacctc cgctcccgcc 900tcgtctgtgc ccagggagca gggtggaccc tcccttgggc tcttgcctgc acctcccagc 960aggctgggcc tcagtgtcct tacctgtagg atgggtcagg ggcgtcctgg agagagtcct 1020cgggacaatg gggaggctgg gggcaggccc agcctgaccc tgaaggtggg agtgtgtgct 1080ccccctgggc tcagccagcc gcgcttgggg ccgggagggg gtgggggacg tggctggggc 1140aagttgtcaa gggccgcgag gctcaccccc gcccatcgct ccccatgtgg cagcctcttc 1200tgcagcctct acttacccac cctctgaaat gggctgaaaa cacccatctt ggcatgccaa 1260agcttctctg taaaaagcgt tgctgcttct tgatgcttct gaggcccctg cctgccctgg 1320cctctgagcc ctctctctcc tgcctcgttt gggggcaggg agtggcacca tagaatctgg 1380cgctgggcct ggggagcggc cccctcgtgc caggcttccc cgaaaggagg gctgggctga 1440gctcccgacc ctctggaccc cttaccagga ccccttacca ggggcttccc cccccccccc 1500ccccggtggc ggcgggctgg gctggggcct tttccttgca gccgagtcgg agctgtcgga 1560ggcgagggcg aggacgggaa gagaggaggg cgtggtttct gctggtcctc actcctctcc 1620tcccgtcttc ctcctcctcc tcccattccc acctgtgtct ccgggtcccg gggccgcagg 1680ctgcccaggc gcctgctgat ccattgggga ccgcactcgg gtccccgctg gccttcgggt 1740cagggccacg gcccacctat tttccaaaca gccttgggtc gaggcccaag aggctgggcc 1800cggtttaagg acggggaggg aggcgccaag aggccagggg ctggtcccga gcacgcccgc 1860acccgctcac ccccgctgtc ccctctcctt ccccgggggg cccctgtgca ccccactctc 1920acttcttctg ctcgaggcca cgaggctggc tgtccccgca aggtgaccgg gcgtcctgtc 1980tggagggcgg gggccggggc ggctgggggc accgtccgtg cccggggccc ctgtgctgac 2040gtgccctccc cttggtcctg tgggacttcc aggcaggccg gcaagccgcg tgaaccgccg 2100cagccgtggc atcgtggaag agtgctgctt ccgtagctgc gacctggccc tgctggagac 2160ctactgcgcc acccccgcca agtccgagag ggacgtgtcg acccctccga ccgtgcttcc 2220ggtaaggcag cccctctctc ggcagcgccc ccccccgggg gggggctgtc tcctctgagc 2280cgggggaccg gggcgcagcc ggctcttggg cttcaagtgc tgccagaggg gccttccccg 2340ctggggaccc tggccagaag ccagggcagt cttcgctctg tcgcagggca ggcaggcagg 2400aggaccccgc agaggttgtt gttctgggac aggggctggg gggccaggcc cccccctgac 2460gggcccttcc cctctcagga caacttcccc agataccccg tgggcaagtt cttccgctat 2520gacacctgga agcagtccgc ccaacgcctg cgcaggggcc tgccggccct cctgcgcgcc 2580cgccggggtc gcacgctcgc caaggagctg gaggcggtca gagaggccaa gcgtcaccga 2640cccctgaccg cccgtcccac ccgagacccc gccgcccacg ggggcgcctc tcccgaggcg 2700tccggccatc ggaagtgagc caaattgtcg taattctgcg gtgccaccat ccacctcgtg 2760acctcctctc gaccgggacc gcttccatca ggtccccctt ctgagatctc tgtacccttc 2820tgtctgcggg catctccgcc ccgggccccg tgccccaacc tccccatgtc aggctagtct 2880ctcctcggcc ccttccatcg ggccgagggc atccaaacca caaacccaat tggcttggtc 2940tgtatctccc cccaaattat gcccccaatt atccccaagt tacataccaa aaattgaacc 3000cctcaaccac acccacatac aatcagcccc cgtaaaacga attggcatct ttaaaacacc 3060agaaaagcga attagcttta aaaaaaaaat aaacccaaaa tatcaattag ctgaaaaaaa 3120aa 312220630DNASus scrofa 20tactaaaaat aaattggctt aaaaacaatt ggcaaaataa aagaatttgg cccccccctt 60ccttctcttt cttttcggac cttgagttaa attggctgtg acccatcatc caagagaaag 120gaagggacca aaatttgcag gtaggcttgt cgccgctcac agccatctcc ctcctcctgc 180cacaccctcg ccggccactg gcggtgtggc accaaggacc cagtcccgtc ctctctctag 240tcccatgacc gagaccgcgg tggagttggc tgggagaccc cgtgagatca gaggagggga 300gcacggaacc agaaacccaa acctgcacag gtacaacatg actggccccc cgcacagccc 360aagacctctc atctcagtct ccacttaaaa agcacctgta cccacacgca tccctgcaga 420aacacacaca cacacacaca cacacacgca cgcacgcaca cacgcgcgca cgcacgcgca 480cacacacact catgcgtata cacacacaca cacgcacgca cgcgcaccca cacacacaca 540tgcattcaca cacacacaca ctcgtgcata cacacgtgcg cgcgcacaca cacacacaca 600cacactctct ctctctgtgg gatccctgag 63021500DNASus scrofa 21tggctctggc ataggctggc agctgcagct ctgactggac cccttgcctg ggaacctcca 60tatgccgtgg aagcggccct agaaaaggcg aaaaaaaaaa aaaaaaaaaa acaaccaaac 120aaacaacaaa agccaaaaca cacagaactc acagacacaa gaagagactg gtggttgcca 180aaggtggggt cgagggtggg aaaaatgagg agagggggca aaacacacaa acgtgcagcc 240ataaaatggt aaagtcccgg ggacctccgg tagcgcgtgt ggggactcgg gttgagaaca 300caccgtgatg tgtattcgcg agttgctaag agtccctgtt ggagaaacaa atgcgtatcg 360acgtgtggaa atgaaagtta acccgacctg ctgtcgtgat cactttgcaa cacatacaga 420catagaatca ttatgtttta cccctggagc tgacagcgtt atacgtcccc cagcctcaat 480ttaaaaacag cgttgccgtg 50022400DNASus scrofa 22ttcatactgt gcaatgccag ccttaaatgc acagaggaga gcattaactt ctttgcagaa 60tcactgaaat gataccactc atgttttgca acttgcactt gggcgttatt ttattggtgc 120cggaacagcg gcgatgtggc accaaactag cgccgctgtt tttatttccc ctcggtatcc 180gcgctctcgc tgtcttcccc cccttccgct tgcagctgag gaaagggctg agaggaggaa 240agtctgcatt cacccatctc cccctgcctc tgttgtcatc cttcacagaa gtggtggcct 300gtgcggggaa gtcactaaac ctaggcaggt gtcccgtggg gtcatgcttg ttacaccttt 360gtgcacctgg cccaagttct gggtggagcg agaacgtggc 40023559DNASus scrofamisc_feature(549)..(549)n is a, c, g, or t 23agctagcccc cccagccagg gccaggcctc tcctgccacc cgcccagcca gcatgtctca 60agaggagggg gcctctaagg gatgaggacc tgctccagtc ggagacacga agccccgccg 120gctcctcccc gaaagtccag ctgcggcttt cgagcacggc tgcgcccttc gtcaatcatt 180tcagccacag aagtgaaagg cgctttcgtg gccgaggcag gcgggacaca gaatggaatc 240ccaccccaga gcgaagagcc gccgtgggtg aagcgcgtct ctggtgggga ccgggccggg 300aacttcacat gggggtcgct gtccccatct ccccatcgtc attactgcag gggctcggcc 360acacccggag ctgcgggggc cagtgctgga cactggacct ggcctccgtc ctatgatgtc 420atgggggcgg ggccagcaca gggcagtggc cacacctcgg gcctcccagc accagccagg 480atggcagagg gccccacccc accacggggc atgtacatcc cagaggacca gctgagcaag 540gcttgatang ggcttcaac 55924450DNASus scrofa 24cgtgcaggga cccgtgcggg ccttcctgtg gccacagaga acaaacacac cattatcttc 60agccccaccg cgcggcctgt taatgggtaa actggggcaa gggggcccct gcctgaggcc 120ggggtgggga gcgcaaggca tggcctgtgt gccccagccc agtccttcag ggcgctgctg 180tcctgcaccg ggggccccag gaagcagagc acccagcttc tcccctattc tagaaccagc 240ccccagaacc ctggacccag acccaggccc aggggatact gacagagcca cggcaaggcg 300gccactccac accccacaga ggggccagca aaccccagtc actgcgcagc ccatgcccag 360ggggcagatg ggacacgaga gcagccctca tccacagcag gcaggggagt gaactggtgc 420aaaacggggc ggttccacga aagttaagca 45025535DNASus scrofa 25tgccagagac ctcagagctg ggctctgcct tcccgggctg acacggaggg ctgtggcttc 60caccacccca ggccacagcc agcctgccca agtccctgaa gtgtccccag aggtggccct 120gcctccacgc ccaacatcag gcctgctgca gccctggacg gccccctgtc ccccggaagc 180cctcggggct ctctcgcgtc gcctctgggg aaccctcggt aatgtggccc agccgtgcag 240tggccggatc atttgctcag gggggcccaa ggcagggggg tgacacatcc gcaagtaccg 300catatgcaca ggatatggat tgggtgtgga tttaaccttt tcgcaaatgt ctctgccggt 360acaaatattg tttctaatcc tctgcctccc tgagccggtg agtctgcccg ggagctgcgg 420ggagctggct tgctgaacct gccctggccc ccacccccaa gggagccccc ggccagtgct 480gagggcagga agcttgggca caggctgcag aggccagcgc tggcctcagt cacct 53526868DNASus scrofamisc_feature(310)..(310)n is a, c, g, or t 26tattgaagac cctatcatga gttcccagag cggaggggtg gaagcagggg cctacagccc 60actccccatc actccagacc cgtccggggc tggtgtcccc tgccccctac tcctgtctct 120ggtgggcgga cgctcgaagg aggcactctg gcctggagcc tggagggtcc ctgaactccc 180gctgccacct gggccctcgg gctcctcctg cgctgggacc cgcggtggtg ggaagcagcc 240ctgctcagtg ggaggaggca gggctgtggc cgccccgcac ggccctgggg gggacgcacg 300caggacgcan gtgggcgtgt gtgagtccgt ctacacgtcc agccaagggc ggccgcgacc 360ggccagggtg ggcagcccca gcctcagcag ggcgctctct ggggctcagg ctgcgccgac 420gggagatgag gggtgaggcg cagtctgggg ctgctgccgc agaacctcgc ccagctggca 480gctgggcaca gggagacctg tactcccaga acctgaggct ggacgtccga gacccgcgtg 540ccggcctctt gggtgcctgg tcagggtcct ctttctggtt tgtgggcaga acctcctcag 600cgcgtccttg catggggtgc taatcacgga gtaaggagcc agagaatgag gcacggagta 660tccagtgtta accctggagt atggagacgg gagtactaat tgtggagcat ggctctaagg 720aatggagtat tcgtcacgga gaacgcgggg ccgggtgaaa tacggagagc ggcgtacgga 780caacggggac ggggtatccg aaggggagga tggagtatcg gccggagggt ggagaatgga 840cactagagga tgtatanngg gcgtcaat 86827500DNASus scrofa 27accagtttcg atgagcaatc ccagcggcgt aacattatgg ctgcagcctg gtcaatgccg 60gtggagtttg aacctccacg cgtggcgatt gtggtagata aatcgacatg gaccagggag 120ttgattgaac ataacggtaa atttggcatc gttatcccgg gcgttgcagc aactaactgg 180acgtgggcgg tgggaagtgt gtcggggcgt gatgaagata aatttaattg ctatggcatt 240ccggttgtga gaggcccggt atttggtttg cctctggtcg aggaaaaatg tctggcgtgg 300atggagtgtc gattgctacc tgcgacttct gcgcaagaag aatacgacac gctgtttggc 360gaagtagtat cagcagcggc agacgcacgg gtatttgtcg aaggccgctg gcagtttgat 420gatgataagc tcaatacgtt gcatcattta ggtgctggga cgtttgttac cagcggcaag 480cgtgttacgg cgggttaagc 50028900DNASus scrofa 28atgtttgatg tccgcgcgtg ctgtaaaaat ttacgctgct cgcgttcttt ggcttcgtcc 60accaccggaa aacggacaaa aatttccgtc ataccttttt ctttcaggcg gaagccaatg 120tcgtaatctt cagtaagact ctgcacgtcg aaagcaatac cgtcaccgtc agctaacagt 180gcggtcacgg cgcggcggct gaaacaggtg ccgacgcctg cgctgggcac ttgtccggcg 240agggcttcac gcaccggaac atctttgcca tgcagctctg aaaactcatc aatgtaagtc 300atgctggtga agtgcgtcca ttcgcgttcg aacggataca ccgggatctg aatcagatct 360ttacgctcga ccagatagtt gaacagacgc aattccatcg gtgaaatcac atcttcggcg 420tcatgcagaa taaaaccagc aaaagcgaaa ttggcgctac gctcaaattg ggtgatggcg 480tccagcacgt tgttcagaca gtcggctttg ctggtggggc caggacgcgc gcagactacc 540ttatgcacat tcgggaagcg agcgcacact tcgtcaacat cacgctgagt atcggggtcg 600ttggggtagg tgccaacaaa gatatgatag ttttcgtagt cgagcgtggt cgccgccagc 660tcggccatat tgccgatgac gcccgtttca ttccacgccg gaaccataat cgctaacggt 720ttttcatctg gtttatacag ttcgcggtaa ctcattcgcg ggtagcggcg ataaacactc 780aacttgcgtt taatgcggcg tacccagtat acgacatcta taaaaaaatc gtccagcccg 840ctgatgaaca tgatgaccgc taacgttatc gcgattactt ttaagccgta tagccaggta 90029500DNASus scrofa 29agctggatgc ccccagctgt ggtcccttcc cttccctcag ggcaggttct gtccctcttg 60cagccaccgt cactgctgtg gacaggtctg cacacccgcc gtccaccaag agcgtggcag 120gtccctgggc acgggccggc tcctgacgca ccatgtgttc aaggcaagag cactggacag 180agggtccaga cgtccccttg tcctgctcag gcctgggcgg gggcagccct ggcgggagag 240gccctgggca tcagagcctc tgtggcctgg agcttggcgc cctgccctcc ccacctccgt 300cctgctcctc gccgcgctgc acggacctct cccggccccc caggctcatt actcttaagg 360accctagccc cctatgctga aatgctgtac ctcgtgcttg ttttcatctg tttattacct 420tatcttcatt cctgcttgat gatatctggt tattctttat tgattatata tatcttgttc 480gtgtttttat aggacactgt 50030450DNASus scrofa 30agtgcggtcg ggccgtcctg acgctcaaca ccgtatttcc acgcgaccgc ggattcaacc 60tggtcacacg gacgccatgt agacatgttc ggggttacgc gcagagaagc gacctgctca 120accggctggt gagtcgggcc gtcttcgccc agaccgatgg agtcgtgggt gtaaaccatc 180acctgacgct gtttcatcag cgcagccata cgtacggcgt tacgtgcgta ttccacgaac 240atcaggaagg tggaggtgta cggcaggaag ccaccgtgca gggagatacc gttagcaatc 300gcggtcatac cgaactcgcg aacaccgtag tggatgtagt tacccgcagc atcttcgttg 360attgctttag aaccagacca cagggtcagg ttagacggcg ccgggtcagc agaaccgccg 420aggaattccg gcaacagccg gacgaacgct 45031450DNASus scrofa 31tcaggccaat ctgtctggtc tccaatgggg acaatttggt tctttaggct tctgtccaat 60ggtccgaatg gcccactccc cgggcgccgg ccaagggtcc tctgtgcctc gggtgggctg 120gcacggaccg cccccagggt cgtgccagcc ccgtcaccgg ggcccagaag cttcgggcct 180ctagctggct agtcgggctg ctgtgcaggg gggctgcgct gggggcagag gcgggggtga 240ggtaaacctc ccagccgccc ggggtccctg ccgcagccct aggcgccgag acggtggctg 300ggtcggtacc gccagacccg agggcctcgg ggcccgggtg accccagctg tcgcacacgc 360tcgcagctct cttgctcatc agggctcatc cctctggacc tctcctactg ccccacctca 420ccccgcctgg accccatgaa gccccgcgga 45032600DNASus scrofa 32taaaactagc tctagtagaa acattttatt taaaaataaa aaacctgact acgtcgggag 60ttcccgttgt ggctcagtgg ttgacgaatc cgatgaggaa ccatgaggtt gcgagttcga 120tccctggcct cgctccgtgg gttgaggatc cggcgttgcc gtgcgctgtg gtgtaggttg 180cagatgaggc tcggatcctg cgtggctgtg gctcgggtgt aggccggcgg ctacagctct 240gatgagaccc ctagcctggg aacctccaca tgccctggga gtggccctag aaaaagggca 300aaagacaaaa aaacaaaaga aaaaggaaaa taaaataaaa aagactatgt aaatgaaatt 360aacgactgcc tagggtggga tttacagcat gggaagtaca gcatggccgt gacagtgcaa 420gggtgaggcg ggaaaatgga aataggttag gtgagtttct cctgctattt gtgatgtggt 480ctgctatcgc ttgaagacgg actgcagtga gataaatatg tacagtaagc atccgaaaaa 540ccgccagaac ggcaaaacga atgactccaa gtaagaaccc aaaagagaaa aggaaataat 60033450DNASus scrofa 33gcgcgggcgt tccggctggg gtatttaacg tggtcaccgg ttcggcgggc gcggtcggta 60acgaactgac cagtaacccg ctggtgcgca aactgtcgtt taccggttcg accgaaattg 120gccgccagtt aatggaacag tgcgcgaaag acatcaagaa agtgtcgctg gagctgggcg

180gtaacgcgcc gtttatcgtc tttgacgatg ccgacctcga caaagccgtg gaaggcgcgc 240tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgc ctgtatgtgc 300aggacggcgt gtatgaccgt tttgccgaaa aattgcagca ggcaatgagc aaactgcaca 360tcggcgacgg gctggataac ggcgtcacca tcgggccgct gatcgatgaa aaatcggtat 420caaaagtgga agagcatatt gccgatgcgc 45034450DNASus scrofa 34ggtggatgct ggcgatagcg tcatcctcgc ttatgccgtg cagcgggcaa ggataaagcg 60cgcgataaac atgacccggc atcagcccca tgcccgcaga gtacggattc accttgccgg 120tcagcgccag cgtgtaatgc gtgcgcccgt gatacgcgcc gctaaaagcg atggtgccgc 180tacgtttggt ggcggcgcgg gcgattttta ccgcgttttc caccgcttcg gaaccggtcg 240taaccagcag cgttttcttg gcgaaatcgc ccggcacctt ctgattcata atctcgcaca 300gctccagata cggctcgtaa gccagcacct ggaagcaggt gtgcgacagt tttttcaact 360gcgcttccac cgcggccacc accttcggat gcaagtgccc ggtattgagc accgtaatcc 420cgcccgcgaa atcaagatac tcacggcctt 45035500DNASus scrofa 35acgtgaggtt tgggggagga aagcggggga cgagcagccc gagaggagtg ggggctggcc 60tgtggctgat gaaactctga gaaggttaag agcccccatt tttgtcttcc tcttttttat 120tatggaaaat tccaaatgga tgcaaaagtc ccaaacctaa ctggacatct tcttggtacc 180aggaacggtc aggcacttat gatgcaccga gccccgaggg aaaaaccctg ccgtcctgga 240gcccacggtc cagcagggca cacaggcccc agcccgcaag cggcacggct gagtcagtga 300atggcgtgcc ctctggtcaa ggacgggcac tctggacccc agggaagcct ctgaggagcc 360cccttcacag cgtcaaaaac tgttaacagg gccatgttcg caccccccca cacacgtggt 420tcagaagcag accccaggca tcgtaatatg tcatccgtga gttccctgtg tgccaccaac 480agaaagccca tcgtcacgtt 50036400DNASus scrofa 36cggcatcgat gtacatggta cgcaaggcac tcgtaaggcc ccgagcctct aggccttgtc 60attgtcacgt gctgctcgcg gggatcagca gccaggcttg tgaccccggc cactttgaca 120gataaggaca cagagaggcc acagcactgg tgtgaggccc cacagccagc agcccagggc 180agggaggact gggtctcacc tgcctcagct gggcccagcc tccctgggag tcccggagtc 240tccccagctt aggagtgtcc ctggaaccct cttctctccc cttcccgccc tcacccggac 300cccctgcctc ccccccacca accccctccc cctccttctt tcaccttgag ctcccctctg 360aggacctcta ctgttcctgc ttatcctccc ctttgagcca 40037500DNASus scrofa 37tggcggtgaa ctatgtcgtg cgtgaagagc atttgtggtc ggtagcgcgt tatatgcggg 60aagtttaggc gaactggaca gcctgggttt atccggtagc gaaatccgct ttcacggtaa 120aacgctgcta gcgctggtgg aaaaagcgca gacattgccg gaagatgcct taccgcagcc 180gatgcttaac ctgatggaca tgccgggtta tcgtaaagcg tttaaagcga ttaagtcgct 240gattactgac gtgagcgaaa cgcataagat cagcgccgaa ttgctggcat cgcgtcggca 300aatcaaccaa ctgctgaact ggcactggaa actgaaaccg cagaacaatt tgccggagct 360gatttccgag ctggcgtggt gagctgatgg cggaagcatt acacaattta ttgcaggaat 420atccgcagta aaatcttccg aagccggact gggcgcgctc agcgccacat ccggcttcgg 480caaactacaa atccaacacc 50038500DNASus scrofa 38gatttcacaa gcctgaccca cgcggaaatg cgctaacagc gtaaagtcgt gcggccagaa 60ttttttcgtc tcttcgcttt gcgtcaattc aaaagtcagc gctacgccat cagcatcttc 120atgatgtgat ttcagcgtcc acggcaggtt gcgggcaaaa ccgtgcgcag gcagaccttg 180ttgtgccgcc ggaccaaacc acggccagca aaccggtacg ccaccgcgaa tagcgacgcc 240atttttgaac ggtgtgttgt tgctcaacca cagaacttct tcttcacccg caggtttcca 300cgagagaagg tgtgcgccct gtaatgcaaa agaggctttt acctggggat gatcgaccac 360aatgaggtcc agttcatcca gtttacgacg ggagaggaca ggggagattt gttcgatgac 420cggaagggca aaaattttct taatcatgac gcagtccttt aacttcattt tatcaggtaa 480aaaaaagagc gaccgaagtc 50039300DNASus scrofa 39acctgatcag gctctgcact gtgttcatca gcggagccga gatatttgac cgccccatgc 60ataacggaaa ggcgtgggta aacccccggg cgcgttcctt tatcaagatg acgttcgaat 120attccggcag gtgcagtttg tttattccag aaaggcgttg agcgcgtatg aatataattc 180tgtgggattt gaagcatcct tttccctcct tcggtgaatg cgctgaaaac ggcttattcc 240agccggttca gggtacgcct gataatttgc attttaaata ccatttattg ggtacttttt 30040450DNASus scrofa 40atccttttgg ggtctggcaa ttacgcaata aagaaggccc ccatgcgatt aaagtcaccg 60gcccactgtc gtctaatcat ggagaaattg tccatcagtg gggtctcgat gggcagggga 120ttgctctgcg ttcctggtgg gatgttagcg aaaacattgc cagtggtcat ttagtgcaag 180tgctaccgga atattaccag ccagcgaacg tctggtccgt ttatgtttca aggctggcga 240cgtcagcgaa agtgcggata acggtagagt ttttacgcca gtattttgcc gagcactacc 300ggaatgtttc actgttgcat gcctgattta tgattcaatt atcgggttga tatcagttta 360aaacctgatt ttctcctttc taagccgcta cagatttggt agcatattca cctttaatcg 420cgcatgatct aaagataatt gaagaggtta 45041450DNASus scrofa 41aatgtactgg caaaaagcca atggcgaagc gtggggaacg ttacatgctc tgctggcgga 60tattaatagt cagggtcagg tgcagatggc gatgaacggc ggcatctatg atgaaagcta 120tgcgccgctc ggtttgtaca tcgaaaacgg tcagcagaag gtggcgttaa atctcgcttc 180aggtgaaggg aatttcttta tccgtcctgg cggcgtgttt tatgtcgcgg gagataaagt 240cggcatcgtt cgtctggatg ccttcaaaac cagtaaagag attcagtttg cggtgcagtc 300agggccaatg ttgatggaaa acggtgtaat taatccgcgt attcatccca acgtcgcctc 360aagcaaaatt cgtaacggtg gttgggatta ataaacatgg gaacgccgtg tttttgttga 420gccagcaggc aacaaatttt tatgattttg 45042400DNASus scrofa 42gacattaatc atttcaaaat caaagccccg gttttccatc gcccgtttgg tggcgtggca 60ctgaacgcaa tcgttacgag tgtaaatagt aatgcgcatg attcgtattt ccgtttaaaa 120tgaagatacg gcgcgatgat acgcgtcggg ttgtctctct gttgatacag agatactaga 180tgtagttgaa aaaagattca accacacaat atatagccca gtaggggtcg aaattaccct 240ggatatgagc gtgacggggt agggggattt ttgtgattca ccaggcaaaa agaaaccccg 300aagacaggct tcggggtcaa agacgcgtat ttattatcat ttttgcacta cgatttgcgc 360atgcttaaca gtgcgccgat taaaatatct accgcagctg 40043500DNASus scrofa 43gcaaaatcac gtccgcgacc tggcgttgtc gctgggccat attggcaaag gagctggatt 60gcggtgcctg caaagtgccc tgaataatgc cattgtcctg taccgggaag aaacctttcg 120gaatgaacac ccacagcagc acgctaagca gcagcgtgct gagtgccacg cttaaggtca 180gccacggatg attcagcact ttcgccagtc cacgaccata ggcggcgatt atcctgtcga 240acattttttc cgaggcacgg gagaagcggt tctgtttacg caacgactcc tggctgagca 300tccgcgcgca catcatcggt gtcagggtca gcgacaccac cgctgagatc aaaatcgcta 360ccgccagggt aatagcaaat tcgcggaaca gtcgcccgac gatatcgccc ataaacagca 420gtgggatcaa caccgcaatc agtgagaagg tcagcgagat aatggtaaag ccgatttcac 480ctgcgccctt gagcgccgcc 50044400DNASus scrofa 44agctatctac ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgtttgg 60cgccgaaata ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc 120ggatgcagta cgtcaaaccc gaactggggc cggaaggatt tagcttttct gcaatacacc 180ggcggcacca ctggtgtggc gaaaggcgcg atgctgactc accgcaatat gctggcgaac 240ctggaacagg ttaacgcgac ctatggtccg ctgttgcatc cgggcaaaga gctggtggtg 300acggcgctgc cgctgtatca catttttgcc ctgaccatta actgcctgct gtttatcgaa 360ctgggtgggc agaacctgct tatcactaac ccgcgcgata 40045450DNASus scrofa 45gattagcgcc agatgctcgc catcgaaaag ttgaatcaac cccagctgcg ggtaataagt 60gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga cggcttcaca 120caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt tttctcttta 180gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta acgctcaggc 240tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac 300gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt atcccgtgag 360ctgacggcgg caaaaagtca ccagtgactc ttcggtaagc gatggatctt ttttcactac 420gaagattttc accgcttcac cactggagcc 45046750DNASus scrofa 46gagcagcccg cgtgatgaca ggcatgcgcc cgcgtcggct ctctctctct ggtgcactga 60gtcacaggat ggcggcggtg ggcgcggtgg tggaagcggt cctggagggc tcgggaggga 120ggatgcgctc aagctggctc cccgtggggc tggcccggag tagcctccgt gagggcaccg 180tgtctgctcc cagagcccgc tccccggcct gccctgcctc ccttccctgc cccagttccc 240ccggagcccc tggatcccga tgggaggcgc ccctggggag aggggaccag ggaggggccc 300agagctctga ggccaccaga cctggccagg acccttcgtg ggaagaagag gtgggcccca 360aaggcaccta gagagaggga ggctctgctg gctggggggc cttccaggcg gggcttccag 420gcagggccag tgtcctgggg gctggaggga gtccctggct gctggggggc ggcaggagca 480cctggggcgt ctgggaagag agcgggagga gactggagcc aactgggggg acagaggagg 540ggtccaaccc cagcggtggt gttgggggtg ctggtggtgg aggccctgag aggctgtgct 600ggggggcaga gcgggtgctg ggaggggaga aggggtcccc agggctcatg ggcccttcgc 660agcagtggca gttggggtgg gtggctgtct ctagggctgt accacggtgg gtgcctggag 720aaagaggtcc tacccctagt ctttgctgca 75047300DNASus scrofamisc_feature(299)..(299)n is a, c, g, or t 47tggggacccc actccagccc cactgagtga cgcgcccccc tgtggtccca ccgccaaccc 60tgcctcacac cagaggggct gtggccacac cttgtccaca gcctgtccct gagaccacga 120gcccccgggc tcagccccct cctcacccct ggaccgagga gaagccccca cctgggctca 180gctcttggag ctaaacttcc aggaaggttc tggtgccctc gggtcttaga gcatggtggg 240gagggggatg ctggtggggg cgcaagccct ccccacattt cgcactcgac ccggtgggng 30048300DNASus scrofa 48ccggctagaa gccacgagag cccccaggcc ccgcccgacg tctctcctgc agggattcgg 60cagccctggg gccacagggc ctgagcagac cttggggttc cggtgtgact ccagccaggg 120tccctactgt gtaggcacca gggcagagtc agccctggga ccatggccac agctgctccc 180gcctgagccg ggccccccgc ccaggctggg ccccctcagt gcactgtccc aagccagctg 240ctctccccac ctccaccttc tccatccagg tcctgcccca cggcctttgc tcaggcccag 30049500DNASus scrofa 49ttgactggca ctagcacgag ctctgtaccc ggggatctgg gctcgggaga agggagaccc 60cccacccggc aggccgaggg cgctgtcaca ccatgactct cagccttccc cacccgacgg 120acaagagtga ccctctccca agcccccact cacccaggac cgcacacccc gtgagtcctg 180cgagtggggg cggctcaggg gccccgagtc ccaaaggagt ctgctggccc tgggggggag 240gggaagcagc agggtggtca cgggtctccc tggttggcag gaccacaagc tcagcccgct 300gcctcccaga gggcagccgg acaccaacca gtccggggac cccacgtacc tcagctgctg 360caggtgcccc tgcctgtact ggtgccaatg gggccgctgg gtgctcccat ggacagctcg 420ccactcatcc cagccgccta ccccccttcc gggtccagtg tccggccggc cacccgcctg 480cccagccctg gcctcctctc 50050500DNASus scrofamisc_feature(500)..(500)n is a, c, g, or t 50ggggttgccg caggctgctg tgtaggtcgc agacgcagct tggatctggc gtggctgtgg 60ctgtggctgt ggctgtggca taggtcagcc actgcgactc cgatttgacc cccagcccgg 120caactcccac atggcacagg tgcagcaggg aaaataaata aatgaaataa aaataggtga 180agacagtgga tttcatctct tggggttgcg gtaagctcta cacaataggg agtttaccat 240tttacctgtt tcaagtggca ctgagtcagc tcacagtcct gagggcccac agatgccgtc 300tgcctgggag attgttcctc tcaccacact gcccctctgt ccccactaaa tactcactgc 360cctccccgtc ccaagggccc ctgccccacc ctctgcttcc tgtctctgaa cttgctggcc 420accagcgacc gtctggtgac ctcactcttc ggccccattt gtcgcacacc ccacctggcc 480tctccccggc atgggcagan 50051600DNASus scrofa 51gggatatttg ggggcatatt tgggggggag atccccacaa ggcatttggg gtttgtggtt 60tggaatgccc ccgggcccga tggagggggc cggggaagaa tctaagcctt acttggggag 120ggttgggccc cggggccccg ggccggaaat gcccccaaga cagaaggtgt acaaaatttc 180tcaaaagggt gacccttaat gaaacgggtc ccggttggaa agaggtcacc agggtggatt 240ggtggcaccg cagaatttac gacattttgg ctctcttcca atggccggac gcctggggat 300aggcgccccc gtggacggcg gggtctcggg tgggacgggc ggtcaggggt cggtgacgct 360tggcctctct gaccgcctcc agctccttgg cgagcgtgcg agcgcggcgg gcgcgcagga 420gggccgcgca ggcccctgcg caggcgttgg gcggactgct tccaggtgtc atagcggaag 480aacttgccca cggggtatct ggggaagttg tcctgagagg ggaagggccc gtcagggggg 540ggcctggccc cccagcccct gtcccagaac aacaaccttt gcggggtcct cctgcctgcc 60052179DNASus scrofa 52atcttcatat tcatgcagaa gacactctcc tgcctttcta tcttggggaa aaggacgatg 60tcacttatgc aataaagccc acttgctggc cggggcttga cattattcct tcctgtctgg 120ctctgcaccg tattgaaact gagttaatgg gcaaatttga tgaaggtaaa ctgcccacc 17953500DNASus scrofa 53ctcgggctgc ttccaggggg ccttggggag ccatagaatg ctatggagca agagagtgct 60atggtcagac gactttgggg gaaggtctgg gagaagaggg gtgactggcc actgtgataa 120agagtgggcg cttccttgag ataacacggt gggcagccga ggtggacctg tgcaggtgga 180gaaggcctcc tgccgcggcc agtacgtggc tctgggctgc cggacacgag aaagcccacc 240tccacggctg cctccaggcg gcccttcctc tcttcacacc gccgggccat gcccaggtgc 300aggtgccatc agagggtgct caagagaagc tctgggctgg ggttgtccca ggtcccggaa 360gccccgtgtc ccaggggcca cctgaggaag cgtgggcgca cagagactgt ccctcggtgc 420tcagagaggg tcccgtcccc acggcaacga cgcccaaggc ggaggtggtc agaggtcttg 480ggagggagga tggccgcgca 50054900DNASus scrofa 54tgtgttgcac ctgttgctgc ctgtcgactc tagaggatca atactcctta cataattaag 60gagaacaaaa tggaacttaa aaaattgatg ggacatattt ctattatccc cgattacaga 120caagcctgga aaatggaaca taagttatcg gatattctac tgttgactat ttgtgccgtt 180atttctggtg cagaaggctg ggaagatata gaggattttg gggaaacaca tcccgatttt 240ttgaagcaat atggtgattt tgaaaatggt attcctgttc acgacaccat tgccagagtt 300gtatcctgta tcagtcctgc aaaatttcac gagtgcttta ttaactggat gcgtgactgc 360cattcttcag atgataaaga cgtcattgca attgatggaa aaacgctccg gcattcttat 420gataagagtc gccgcagggg agcgattcat gtcattagtg cgttctcaac aatgcacagt 480ctggtcatcg gacagatcaa gacggatgag aaatctaatg agattacagc tatcccagaa 540cttcttaaca tgctggatat taaaggaaaa atcatcacaa ctgatgcgat gggttgccag 600aaagatattg cagagaagat acaaaaacag ggaggtgatt atttattcgc tgtaaaagga 660aaccaggggc ggctaaataa agcctttgag gaaaaatttc cgctgaaaga attaaataat 720ccagcgcatg acagttacgc aatgagtgaa aagagtcacg gcagagaaga aatccgtctt 780catattgttt gcgatgtccc tgatgaactt attgatttca cgtttgaata gaaagggctg 840aagaaattat gcgtggcagt ctcctttcgg tccataatag cagaacaaaa gaaagagctc 90055450DNASus scrofa 55ccagccacca gctggaccct cccggagagg ggctgcctcc tctttcccgc ccagacgccc 60cccagcaatc tgtggccaag agggagtgat accgaagatg gccacatggg ggcgccagcc 120cacagggaac cccaggaagg cgctggaccg tcaggagtca gggctgctgt gcacccatgt 180ggcctgggga ctttccacag cctggtggag atggccgggc acaccgctgc ctcgggggaa 240cgtgcacacg ggtggtacat gtggccggag cccagggcac agggtgaggg gagaagggag 300catgcgggtg cagactcgga gcccgcgcgt gaggtgctgg gtcctcagga cacgctctgg 360gagtggagga cccccatcca cgccctcacc cagtgtgtgc ccgcctgctc ccccggaaac 420cctcacagac acgagggcac acccagcccc 450561133DNASus scrofa 56atggcgctca ttagaattcg acctcggtac cttgggatct tttgacccct acctcacgcc 60atctacaaca tttacctccg aatgaatgag agacaccaaa agcaaattca tagaagagaa 120aaaaaggtaa cctggacttt aaaaatgtaa acttctgctc tttaaaaggc agtgctaatg 180aagttcaaat acaaaccaca gaccataaga aaatacttgc aaatcttgtt ctgacaaaga 240ctagtgttca gaacatacga cgatcaggga gaggaaaacc agcaatccta taaaactgga 300caaagaattg gggggaaaaa aaacccactt ggccaagaag ttggtaaata aggccatgaa 360aacatgctca acatcatgag tcattagaaa aatgcaaatt aaaattataa tgagatacta 420ctacacagct atttgaatgg ataaaaaatg ttttaaaaac tgattatacc caggtttggc 480aagaacatga gaaacgagat tttcacacac gattggtgga aaacagaaaa tggtccaccc 540actttggaaa agagctgggc acttccctca aaagttaaac atacatccag gacctcacac 600aggctttcca ccacaggtgt ttattccaga gacatgaaag cgctcatcca cacaaagact 660cgtaaatgaa ggtttatagc accgtttgtg gcccgaactg agaaaaccca aatgaccttt 720aaccagagaa tatctaaaca aaatatccat tcacattaat cacccataag aaggaacggg 780ctatggggac gggaaccgta ttgaagaggg tcaaaataca tacgcagcat caaagaagcc 840tgcccaaagg acacacactg cagggttcca tggactgaaa ctcgagaagg tgaaaactcg 900ccagcagtga cagagagcag gtccgagatc aacctgatgt ggaggaaagt gaaccctcgt 960gcgttgttgg caggactata aactggagca gcccctacgg acaacagtag cccgggctcc 1020tctcctccat ctccctgggg agcctgagcc ttgagacgct ggggcaagtg cacggcatgc 1080tgcctcacgt ggggccccgg tgaaaacacg tggcagctgg ggaaagaatc gta 113357735DNASus scrofa 57tactgcctgt ctctatggac ttgactcctc tcgggacttc atgcgaggga tcttacagaa 60tttgtccttt tgcatctggc ttgtttcact gagcatcgtg tccccaaggt ccatccatgt 120tgcagcctgt gtcaggattt ccttcctttt caaggctgaa tagtactcca ctctgcggat 180ggaccacgtt ttgattatcc atactagtaa atccatacta ataacttgtt cactgaagcc 240cacagcttat gctaccttcc gtgggctcct ccctgccctg tctctacgcc ttctgctata 300gccccatccc ctctcatcca ggccacgcct cctgtcccct ggacactgtc ccagaagcca 360actgccctct gactgctgct ctcgcgtgac ggaggacaag gcaggctcag gggtccacgg 420gctggggccc cagggctccc catggctggt gccccttcct gattccagaa gtacagtggc 480agcaccagct ttccagctgc cccaccttct gtccgcaggc tgctcgggtg ggggcaggtg 540ggcagtgatg tcacctgctg taaccaccct accgtcgctc atccctgtcc aggaggtcac 600ggtgaccttg gcaaacattc tgaacaacac acacctccct ctgcttagag gccgggggcc 660tccccgggtg actgggggca caggctgacc ccagcctgtc tctgttctct gaaggacatg 720ataagtactg caaca 73558500DNASus scrofa 58aggaagaaca ggaaacaacg gggttgagga gaagaaacgg gtgtctggca ggggcacgtg 60ccaacggtcc accgggtgct gccgcgctgc ggcctggcgc cagagggggc agctccgccc 120ctcgggccgc gccctgccgc ttgtgctggc tcgcggctgg gctctgcttg gctgggttac 180agctgggtgc agccgcaggc tgtggtgggt gccgccgggt cagccagccc ggccccaccc 240ggcccgtctc gccggcctgg cccgggcagc cctcctgcag tcgaggagtc gccctgacgg 300gctgattggt ccacagcctc agatgcaaac cagccccacg tgcctggagc cagccagccc 360gggacaccct ggtggaggca ggaaggcagc agcctggaga gccgcgccgg atgatgctgc 420ggggaaaccg ggctcccgcc gggggcgccc tggctctggc caggcttggc ttgaatgctg 480acgtgagcgg tggccctata 50059500DNASus scrofa 59tggcgttgca gtggctctgg cggaggccgg cggctacagc tccgattgga cccctaggct 60gggaacctcc ataagctgtg ggtgcagccc taaaaagcaa aaaaccccaa catatatata 120tatatatata tataattatg gtaaaataca cataaaatag aatttacctt cttaataatt 180ttcagtgcac aattcagtgg cactaagcac attcatgcgg ccgtgtcacc tgctccagaa 240ctttccatct acccaaacgg actctccgcc ccatggaaca cgccccctgc ccctcccccg 300gccctgcccc gccagctcct ccctgtgtct gtggatccgg ctcctccagg gaccccgtgc 360gtgggctcac agagtgtgtg tccctctgtg accgatcgtc gtgtccccga ggcccgttct 420gtggcagctg cgttatgacc gactaccttc gaatgctcag tgactgccgt gcattggaca 480cgcagtccgc tacccttttc 50060550DNASus scrofa 60tgctttctgt gcccccctcc agcttgggac cccagcaggg caaggggtgt atagggctta 60aggaggcagg gggcgtctcc tcccgctggc tgcccagagc acccccagcc ccgcctgccc 120ctcgtccatc tccagcctgt cctttcctgt gccctccctg tcccgggcgg gccgcacact 180ggcttccacc tccccaccca actggcggcc cggtccttcc tgctgaggca ccccgaggtc 240cccgctgctg gggaccagct ggcaggtggg tcccactgct ttctcagcgt gggctttgga 300ggggggatct gcacatacca tcccttcagg ccccgtgggg agcctgggga ccatccggga

360cccctgtggg caggcccaga ggactgccag gaagagaccc aggggaccag gcagctccca 420ggcctctcag cttcaggcca ggggagccca cccccaggtg gcaggtgaag ccaggccccc 480aacccacaaa actgcccgca gggaagtagg agggacagga ggaggggagg ccaggcccgg 540gccgcccttg 55061819DNASus scrofa 61tgaggagcgc aggcccaggc ctgagtgtgc ccagcttaca cccctggcag cttcgtccct 60cctggcccta acccccatcc taccccagca gcaggggctc ccccggtggg gcctggtgag 120cgtctgactg gggtttggag tcaggtctgc tccaggctca gcccccatcc ccaagggtgc 180cctgcagcac tgctgcccac cccctagcgc ccccagacct tcgcccctcc agcctggatg 240tacccacgga ccctgaaaag tggggctgag caggtgccct ggctggagtc cccctgactt 300ggggctggcc aggctgccct ggaggggctg tgggggcaca gcctgcccca ggggcccgct 360gggcactggc tctggagctg acgacaggca ggccctctct tcctggcggg gccacaccct 420gccctggggt ttggggccaa ggcgggcacg ccccatgtca ggcgggggcg aaccaggtaa 480ttacagcctg gcagcccgct ccccagaccc ccagccccgg agggccccca cccaggctgt 540gccaccaaga cctggcatcc agggcccaaa gcaggtcaag ggcagctgct acagattctt 600ttaagttgag acagaatcga cacatgacaa gttcctggtt ttaggtactt cgctgccggg 660gccgccagtc agtttagtga cccagcacac cccacacagg tacaattgct cttctcaaaa 720gaggcccctg agagagcgcc tgtcttggct caggggtaat gagcccaatg ggtatccatg 780aggttgcggg ttccatcccc ggcctcgccg cgttggtta 81962500DNASus scrofa 62ggctcaggaa gcgcaggggc agcgtgtggg gcgacgggaa ccatgggggt ctgtcttccc 60gcctctcctc aagcccaccg ccctgctgcc cacctccgac tctgcagcca gcatgccggc 120tagagcccct gtgcacccag ctggtggcct ctggctaagg gcagtgctgg ctgtggacgc 180gtgtcccctc cccagcagcc caagggtccc atctgccagg ctggtggctg aggtctgccc 240tgtgtggtcc ttgcaaaaac cccgccctct cctgcccctt gaggcgtgag ggagacgcgg 300gctgggcgga tgccctcggg cacagccgcc cgcggtggcg ccctgtcgag gagggggctc 360cgacgtgccc tgacggccct ggccgggcgg agagggtgag gccacctcct ggccacgtcc 420acccagctgc cacgccgcct agccagtggc ccggggccaa gtcagcagag ccaggcttcc 480gacaagcaga ggctgtaggc 50063700DNASus scrofa 63gatgaggaag ccgctgctcg tgctgctcgt cttcttggcc ttggcctcgt gctgctatgc 60tgcttaccgc cccagtgaga ctctgtgcgg cggggagctg gtggacaccc tccagtttgt 120ctgcggggac cgcggcttct acttcagtaa gtagctcagc ggggcacggg ggcggggcgg 180acacagcagg tgctccatcg gtgctgcccc ggtacctgtg cgggtccttc gggatggatg 240gtgtggggga cggggggcgg ggggcggcca agggaggacc tctcctccga gggtctgaga 300cttcagaccg ggggcgccct ggccgtgcgc attgattggc acctgccatg tgcctggctg 360gggctcacac cccctgacgt tcctgcagcg tgactcgaaa cgggaaaccg aagggacggg 420tggcacgggg tggggaggca gaccgtgagt ggcaggcgtg cgaggggttc tttcgggcgg 480ggtggcccag gcaggcccca caggatgaca gcctgtcccc tcctgctcct ccttgacctg 540cccacagcca gggctgcagg cactgacatt cacccatggt attgtggtgc cttgacgtct 600tggcagtggg cattgggttc atggactgtt tggattgaaa agtgggaata agatggggtt 660tgaaaaaccc aattaagaaa taaaagggcg ccctgtgggc 70064300DNASus scrofa 64tttgaaaaat tttgagtcag tgcagaattc gcatctattc cgcattcagg ctctcctgtt 60ctcaccttgc cttagtgcgg atcttctata accaccacag tgacgttttc aaggtacttt 120attgaataat aagaaaaaag tgcacacaat catgtagtta actttctgtg ctctttgcca 180gtttgaaggg accctctttt tttccttttt agggcttcgc cgacggaagt tcccgggcta 240ggggttgagt cagagctgca gctgctggcc tacagcacag ctcttggcgg cgatggatcc 30065450DNASus scrofa 65tcctgggcca caggctgcag cagctcacct gggggctggg gtctcgctct gcggatggac 60ccatgaaggc cggagccagg tgggggccga gacggcaggg caaagggtct gcacacacag 120cgtccccccg acccggcttc tctgggttct tggggggttg gcgaggcttc tctcagtctg 180ggtttcctgg ggaactttca agaactggga agtcttccag aaagttgggg tgaggggagg 240tacccccaaa gtgctgctcc tgtccccatc ccccaccccg ctgtccatcg gcgagacccc 300ggaccgccgt ctccctgccg aggtgtgggg tccccccctc tgccggccag gctgggcagg 360ggtgagcgcc ccctgctctg cactcgggac tcagcctggg gaaggcgggc cccaggaggt 420cctggcctgg acggcagtga ccttccaccg 45066500DNASus scrofa 66tgtgcatcca accccagtgg ccacgggggg tgaccctcgg ccggtcagcc gcccgcgtct 60cccacggaac cgggccttgg cctgaggcag aaggacccag gactccatcc ctgccccgga 120ctctgccgga gggtgcggtc tgcacagaga ccctctgggg gtgaggccgg tcggggctgg 180ggttgagatg ggatggtcag ggcggccccc gcgggcctgc aggaggctgg gtgaaggagg 240gggcccagct cagacgcccc caaacctagc ttgggagagc tgcagccccg ccccgtcaat 300cgcgacagcc tgcccacaga aggcattcaa atgagagaca aatatttggg cttgaagact 360atacccagcc acgtctcttt gggagcccaa gctgctccca ggccctcatt tgggtattaa 420ttggttttcg tttagagatt tgcatgctta tcaatggcca ctgggcggct gggcctggat 480gcggtcccag gctttgtatg 50067661DNASus scrofa 67tcccacgacc tgcccctcca gggccacatc tggcgacacc gtcgcaagag ttggaccggc 60ctggtgtggc cacagcctca ggccttgtct ggccgcccag gccggctcca ggctccaagg 120agctcctgcc tgccctccgg aaccccagca ccccgggccc gcttccccac cagacctgtt 180tttccaggtc aaggtcacag ctaatttggg cttaaactgg acaaggaggc cttatctgga 240gcaggctccc ggccctttgg cctctgccct ggtggggagg ccttcccaga ggctgtgtgt 300tggcgctgac cgtgcagccc tgagcttgaa cccggataag gagggacccc acctgggctg 360gagccagaga gccctcgttc cccagctccg cagggttctc acagtcccgc ccctgccctg 420gggaccctgg acgtccccag caggtgaaag gtccagatgc cctctgacta gaggctcctc 480cgctgtcaga catgctccct tcccgcaccg aggacgagac ctcagcagcc ctgcgtggcc 540tggggtgcgg accccaaggc gtctctgagt gtgttctaat ggggagccgt ggggcctcaa 600cagtgggggt ggcacttgga ggggagcctc cccacagctg ccccaagatg ggccctggac 660t 66168500DNASus scrofa 68tttgttggat gaatgaaatc atgagaaagt gattggaccg ccccgttcgt ccagctgctt 60gccagctgct ttgtaaagat gacctctcac cttctcagag gcctggccgg cccgaggtgg 120cagtcagctg agatgccatg cttgtttggc acgtgggagg cccctgtcca cggcgtgggt 180gcctcttgtg tctaatcagg gtcaggggga gcagcaggtg cagggcacat gtggggccgg 240ggccgatgtc tggggagggc gggaggaggg ggtgtgcgga ggccgttgtg ggggtgcagg 300ggacagaccc cagcgagacc ctccctggcc aggcaccagg acaggtgatg gggggccgcc 360tccggggcgt gtgacagaag cctctcagag gaggccctcc cacggtctct ggaccatcaa 420gggaccgggg gcgctgggcc tgggggtcac acccagctgg ccggccagcc cgggtggggt 480cggaggcccg ggcagttcac 50069550DNASus scrofa 69gggcaggagg ggcccggggc tggtgcggag ggtggaggtg gtgcaggagg gtgtgaggca 60gggctcactg agcgtgcgcg gctggctgtg ccctagagtg gttagcacgt gcccccaccc 120tccagtgtcg ctctgttcac ctgtgcctgg ctcacaggtg tggaaactga gactcgggtg 180ttgcatgagc ttccaggatg agaatcagca ggcttcccag gcagggctgt gtccggggct 240ctgggctctt accaaggagg ggacacccag ggacagccct gcttgggggt gtcgggctgg 300ccaggctggg tggtccttcc tgtggctggc agcccttggc agtcaccccc ttaccctcaa 360ctgcccctca gctgagacac gacctccctg cagagccctg tccacccaga cactcactcg 420cctcctccag gaagccttcc agggctgcct cgccctggtc tcagcaggag acagagagag 480agggtgggcc caggagcaga ggcaggcagc cagaggggaa gcccaggggc cctcactcac 540ccctggggcc 55070500DNASus scrofa 70tttgcattca gctcgtaccc gggatccttc ccgggggctc tgggggtggg ggaatggggg 60tcagaggcag ctgtcatctg cctgtcctac ctgctctcac aggctggccc tggagccctg 120gcctcctcct aggggcacat caggttttgg gggaggccca gcccaccgtc ccacctccaa 180gaccacagct gggagcctgc cccccaagcc tagacctagt ggggctcctg ccagccaggc 240ccccaccttc atgctgccac ccaccaaggt gggacagtgc agccaggaca tccagcttct 300ggagctgccc gaggctcagc acaggctggt accctaggga gcaggtcacc cagggccgcc 360tggcgaggcc tgcggggacg gggggtaggg tgggcagcaa aagaacctct gagctgggcc 420gggcggggtc ggtgagggcc cggggccgcg ggctgtgtgc gtggcccctg agcccgtgca 480gacgcagacc ctgggtgggt 50071550DNASus scrofa 71tgtgctgctg tggctgtggt gtaggccgcc agctgcagct ctgattcgga ctcctagcct 60gcgaacctcc atatgctgct ctaaaaagac aaacataaaa taaaatgggt gcgctgttaa 120tttgaacact ctgcctcctc cagagacgag gccgaaacag gcctctctga aggtcccacc 180tggcagggag gaggaggcca gccccgtggg gggcagagag aagcccgatg tccccagaca 240cacacgcaca gggaccgtgg ccccggctgc cagccccgcg gggggagggc aaggccagag 300actcccagca gcccacagga ccttggtggc cacaggacac aaacacaggt gacggtgggt 360gaggcctggc ctttcccccc ctgggcacga gcacaggaca cacaagagcc ccagcgtgct 420gaccgccacg ccaaggagcc tggatgaagc tggacaccga gagtccacac tgtgtgatta 480ggctgacgtg aagtttaaga acaagcgggt ggctcagcgc ttgaaggcca gaacaaggcc 540gggagggcag 550721300DNASus scrofa 72atgtcaggat agtaacctgg ggtgctgcag tgacaatgcc agatccttaa ccactgtgcc 60acaagggaac tccttgacct agaatcctat acccactgca aatatatttc aaaaaaggta 120aagtcctgag cagaaaagca aaaatgggat aattcatttc tggaagacct tccttgttaa 180aggaagtttt ttggacgtga tgaaggtaga aactcggagg cacacaaaga aagaaagaaa 240gaaagagcac tggaaacgga gcaaataaag gtaaaaataa agttcatctc tttctcattt 300tttaattgct ccaaaagata gctgacctct aaagtaaaaa atagtggaaa tgtagcatat 360gtctctagcg taatttaaag tataacttat agcaatgata gcccaaataa aggaggaatt 420gagaatatac agttgctgtg ttcccattgt ggctcagcag taatgaacct ggctaatatc 480catgaggatg caggttcaat ccctggcctc actcagtggg ttaaaggatc cagggttgca 540gtgagatgtg acgtatgtca cagacgtggc tcggatctgg catttctgtg actgtggctg 600tggtgtaggc cagcatctgc acctccgatt tgacccctag cctgggaacc accatatgct 660gctggtgtgg ccctaacaga cacaaaataa aataaaaata aaagagagag agaatatacc 720attgtaaatt tcctcacatg acacaaagag caatgtgata ttatttggta tatggtgatt 780gattcaagat gtatatcata atattgattc aagatgtata tattcctttt ctaaaaaaga 840gatttataca ataaggcaag agtgaaaata aagtggaatg ctaaagaata gttaatccaa 900aagaaggcag aaaatgggga aaagacatat aacagatgga acaaataaaa aagagctaat 960gagattgtaa aatttaatcc aaacatacag ataatcccat taaatttaaa cactctcaac 1020acattgatta aaagaaattg tcaaattgaa taaacaaagc aagacccaac tagatgcaga 1080ctatgaaaaa cccacttcat ataaagacat gggtaggttt agagcagaat gatggggaaa 1140ccatgtcacg caaacatttg tcaaaataaa gctggtgtgg ctgtattcat ctcagacaca 1200gcagacttca gaacaagaaa cactgcaaag gatgaaagag atactgcata atgataaagg 1260gatcaatttt ccaagtgcag gctccaaaca acagaggttt 130073500DNASus scrofa 73atgacctcat actgaatcga gctcggtatc aggggatctc tcagctgggg gggagggcaa 60tggggcattt gtctgaggat gccccagggc aggcccattg gctggtttgg tgcccatgcc 120ccccccacac cccggcagtg ccccctgctg agcctgggac cccctctggg agttagggat 180tgggggtggg aaccaggctt tgcagtaatt ccagccccca gggcccttcc ctccccgccc 240tcaggacccc cagccccgcc ccacacagtc tccactgtga cagcctcacc ccttgggtca 300agtcctgtcc tctccggccc ccgctgggca gtggagccag ctaggtgaga ggcacaggcc 360actagggcgg tgggcactgc tgaggacaga ggggcctggg tggccttgga cgaggcccag 420cgacgctgag acagtgagcc aggctccagg ctttcccagg gagggtccct gaatgtccac 480ttcttgtgac atcgggtgac 50074550DNASus scrofa 74aagtccatta gggaagggat ttgtgcaaac acagagacag gtgcagggct gggccagctg 60ctgggctggg ggctcctcaa ggcgcccgta aacccctccc tgccagccgc ctgccgccaa 120ggtctgctgt ccaccccggc cgggctgctg tgttcccggc gtgtgtcctg cgaacccgac 180tcccgttcac ccctgagcac tgcctggagg ccggctgccc aggcgggacg ggccctcagg 240gctgggctgg ctcttggcct gtgtttcatt tctgagcagg tccttctcag tggggggggc 300cttgggtgaa gcaggcatgt gcaccactgg ggccctgtcc ccagtgggca tcctgggcgc 360ttgtctggcc cccaaacccc caggccgtgt gcatcatacc ttcaccctga gccccagccg 420aaccccggac atgtgctggg ggaccctggg cacaggggtg agggagcagt ggccttggtg 480gaagcccagc cttggcacct ggggaggggg tgcatctggc atgctctgct gtaaccaagc 540ccagggcagg 55075950DNASus scrofa 75gacgtgcagt agccatgacc tctacggccc ccactgacca gcccgtgtcc ttgtcccgag 60accgacccct aagcaatagg atgcagcaga agtgacagaa cggcctccgc gatgaggtcg 120cagagggctc tggctctgac tcaggcccct catccctcgc tctcctggag cagggccagg 180taggggcccc ccagagacgc cctagaggag gtgacgggca gccagcccgc cccagggaag 240gcctggggac accagggaac agaacggcac aggctcctgg cacagtctcc caggagcccc 300ctggtggcac agaaatcctg accggcccag tggagggggc tggggcgggg ctcggggagg 360agggactggg tgaggccgtc tgactcctgg ctgagcgccg catacttgct gcctgcccac 420gatgccgggc caggccttcc gcacggaccc aggctcacat tcgccctaca tgccactgtg 480tgggagtttg ggatggtgtg cccgctgggc ccgggggtca gggcacgctt cccagaggag 540cgggttccag aaggcccagg tggagaggcg ataggagggc tccagggggc ttcccaggcc 600acctgcgagg accctcctgg ggggaaggga gcggagggag acagccgggt cccttaggcc 660aaggctgagt tgtgaccgca gggagaggag agaaggagca cccacagcag ggcaggggct 720gcgggaggct gtgctgggtg gccgggtggt gggtctgggg gccaggaccg tgggaggcct 780cgagggggga gcaggcacgg gaggggcccc tggacggcag agtccctgct ccagctgccg 840ccccgacccc aggtccacct tcatttcaca gcctggcccc cggccgctct gaccggccct 900gcccatgcag gtgtagcggg gcagtgaggg ccaggctccg gccgtcccaa 95076450DNASus scrofa 76gcaggcctgg cagcagggaa atgatccaga aagtgccacc tcagccccca gccatctgcc 60acccacctgg aggccctcag gggccgggcg ccggggggca ggcgctataa agccggccgg 120gcccagccgc ccccagccct ctgggaccag ctgcgttccc aggccgccgg caagcaggtc 180tgtccccctg ggctcccgtc agctgggtct gggctgtcct gctggggcca gggcatctcg 240gcaggaggac gtgggctcct ctctcggagc ccttgggggg tgaggctggt gggggctgca 300ggtgcccctg ggctggcctc aacgccgccc ggtcccgcag gtcctcaccc cccgccatgg 360gccctgtgga cgcgcctcct gccccaggct gggcccttgc tggcccctct ggagcacccc 420gccccccggg cccaaagcct ttcatgaaca 450771363DNASus scrofamisc_feature(206)..(206)n is a, c, g, or t 77cctccagctg ggcccggcag ggcaccgtgc ccctcagggg acaccacggg gggccacagt 60ggcctctcct gctccaggct ctgctcccgc ctggggcccc ctgggccgcc cgcccatggc 120cagggcaaac tcccagtgcg gctgcccgtc tgggcaaaga ggccgccagg ccccgcgtgg 180tcttagcagg cactggcgga tgccgntaac taaccatttc ttccgcagga gtccgaatct 240gctctgacca cgggccctaa aaatcgctcc tggcccgcag aggatccccg aacagcgggg 300ctgcctcctg ctcctcctgc cgggccggca ctcggcaggc acgtgccctc gtcgtcccca 360gtctgtcaac cgtcccgtcg ttacgatccc cagagtccca cgcgcgggca gctctttcca 420caccccgcac ggccccggag ctgcctgggc acccagatcg cccctgacgc ctttgctcct 480aattctgctg aaatacacat aacgtctcct tgaacgtttg tccattttca cggggacaat 540tctgtggccg taggtacact ccccttgggg cgcagccatc gcaccatccg cttccaggag 600gtcccgtcgt cccagatgga cactgtcccc actgatccct aattccctgt cccccccagc 660cctgcccttc ctgtctctgt ggccctggcg cctccaggga gcccctgtgc gtgggatcac 720aaaacgtgtg tccctttgcg tccggtgtgt gtctctgagc atccggagct tggggtgctt 780ccacgctgcg cctgtgtcag gacgtccttc ccttttgcgg ctgcgcgatg ctccccgtgg 840ggctgcccca cactgcgcgt gttcgctcat ccatccacta aggctgagtt acttttggcg 900gttgtgaata ctgctgtgtg aacacgggcg tgcaaatacc tgctggaggc catgctctta 960ggcctctcgg ggggcacacc cagagcggat atgctcaata aggtaattct gtgtttagct 1020ttttggggaa ccatcaggct ggtctccaga gtgacggagc atgcgtcgca ttcacaggaa 1080tggtgctcga ggctttgagg tctccaccac tcgcttccta ttttctgtgc gtcacagccg 1140tcggaacggc tgggtggtgc ctctgtgtgg cttcaatgtg ctttttcttt tcctggctat 1200gaggttgagc gttttttatg tacttgctgg ccattcgcag ggtttttggg gtttcttttc 1260ttttttgcct ttggggacgg cgcccagagc gtatagaagt tccctggctg gggactgaat 1320cagagctgca gctgccagcc tagcccacag ccgcagcaac gca 136378500DNASus scrofa 78tcatgccatc gccaccgccc cccaccccga cgtttcaaac accagaacca cccctcgggc 60ggcagagaga ggaccggaag gagagacagc ctggtcccaa ggcctcgccc ggtcctgtgt 120ctccgagcga catttctttc tgtttccctc ctccgcggtc caagtttcac ccatcagagg 180cgcattgttt tcatcatctg aaaaaaaaat ctctgtctct taataaaaca caagaaaaag 240tagccttcga aagaaagcac atgaatgata tgtgctggcg acagtgctgg cggcctctga 300gccgtggtgg gaggtgggag ccagcggagc ccctgaccga tcacgtgacc cacgtctctc 360ctgcacagct ggctgcacct gcacgcggtg acacagggac ccagcctcct gccagcaggt 420caccccaccc cgtccgtctc ctgtggaagg ggcagcgttg ccttctgagg gtgggctgct 480ctgaggggcg tcctttggcc 50079626DNASus scrofa 79gccatgggct gcggcggttc acgcggcttg ccggcctgcc tggaagtccc acaggaccaa 60ggggagggca cgtcagcaca ggggccccgg gcacggacgg tgcccccagc cgccccggcc 120cccgccctcc agacaggacg cccggtcacc ttgcggggac agccagcctc gtggcctcga 180gcagaagaag tgagagtggg gtgcacaggg gccccccggg gaaggagagg ggacagcggg 240ggtgagcggg tgcgggcgtg ctcgggacca gcccctggcc tcttggcgcc tccctccccg 300tccttaaacc gggcccagcc tcttgggcct cgacccaagg ctgtttggaa aataggtgga 360ccgtggccct gacccgaagg ccagcgggga cccgagtgcg gtccccaatg gatcagcagg 420cgcctgggca gcctgcggcc ccgggacccg gagacacagg tgggaatggg aggaggagga 480ggaagacggg aggagaggag tgaggaccag cagaaaccac gccctcctct cttcccgtcc 540tcgccctcgc ctccgacagc tccgactcgg ctgcaaggaa aaggccccag cccagcccgc 600cgccaccggg gggggggggg gggggg 62680500DNASus scrofa 80tactcgggtt tgttaccact gagccacaaa gggagctcct aaaaataata attttcttaa 60agccaatgac atggagagca gttagggtgg aggctggtgg gtggtggggc cgcggcaggc 120gccctgaagg tcctgagtgg cacccttggc cgggggaggt gggtgggcga ggggtgttga 180gaaggggcag ggcctcgtgg gggcaggaag gaagagccag tggctcccag tcccctgacc 240ttgctgcctt gagcctggtt ctccccaaaa ttctgtctgt gtcccttcac ttcacggaag 300cttggggccc gttgccaggg agacagatgg gctggtgaca cccaaaatga gccaccagga 360ggggggcact gactttagcc agccggtcac atcaagaagc aaacaggccc cccgctgctg 420taaaggcagc ttggggctgg ggtccgggag caccccctgg gctggggaaa gggggtcctc 480tcaggccccc ggggaggatg 50081427DNASus scrofa 81tctattcgcc gtggccggaa gaggctaacc gtacattgac cgggcatctg gcgatgtatc 60acttctctcc aaccgaaact tcccggcaaa acttgctgcg tgaaaacgtt gcggatagcc 120gaatcttcat taccggtaat acagtcattg atgcactgtt atgggtgcgt gaccaggtga 180tgagcagcga caagctgcgt tcagaactgg cggcaaatta cccgtttatc gaccccgata 240aaaagatgat tctggtgacc ggtcacaggc gtgagagttt cggtcgtggc tttgaagaaa 300tctgccacgc gctggcagac atcgccacca cgcaccagga catccagatt gtctatccgg 360tgcatctcaa cccgaacgtc agagaaccgg tcaatcgcat tctggggcat gtgaaaaatg 420tcattct 42782650DNASus scrofa 82ggcgttgccg tgagctgtgg tgcgggtcac agatggggct cagatcccgc gtggctgtgg 60ctctggccta ggccggtggc tgcagctccg attcgacccc tggcctggga gcctccatat 120gctgcgggag cagccctaaa aaaaaaaaaa aaaaaaagga agaaaagaga agaaagaaaa 180gaaaagacaa aagtcaaaag gagctcccct gagcgatgtc tgtctacgag caggtccctg 240ggagcctgag gcagggtgag cctggacccc tgagggccac tccagactca gtgctctcac 300tggccaaggt ctttggggac cggctggggg cgcgcgcagg ctaaggagga ggtcagagga 360ggggcttcag gctgcagggc cagcggcagc tctgggcccg gggcgggggg gagatggcct 420gagggccttg

cgggggctgg agggtggggg gcttcctgga gtgggaagac gggaagccag 480gtcagaggag aggagcgagg gctgaagctc ctggaaggcg ctggctaccc ccagctggcc 540cgccccgctg ccacattcaa cagccacccg gcctgtggtc ctggcagggt cctggcagaa 600aagccccaag ggccccagcc tggccctctg ggcctaaaga gccaagcccc 65083550DNASus scrofa 83ttaacccacg gagcaaggct ggggatcgaa cctgtaacct cgtggctcct cgtcggattc 60gttaaccact gcgccacgac ggggaccccc cagggctggc gtttccctct gtgtgcacac 120agtggacctg agccaaccag cagggccttc accaccacgg cgcaagagtc ggcagcaaga 180gagcagtgtc tcatggctca ctttctcccc cttccccgga gtggtgacaa aaccccgccg 240ccaccggact cggttagaca aggcggtgcc cagtgccccc gtctgtcacc cgcacggcac 300ggcgctctcc tttctttctc ggggctccac cacgtgtcct cagtttccgc atgagagtac 360cgcggctggc ggggtggtgg ctctggggtc gggggccgtg agggcagggc tgggctgggg 420gaggcaggtc ttggcccatt acgcgggggg cagactccac atcacacgct ctctgtgcct 480cttggctgcc tgacaccatg gacttcaaac aggaacagcc gtggaggcat tgcagcccag 540ggcccgggtt 55084550DNASus scrofa 84tgacacctcc aggcaggagg gtgcaggctg gggtcccagg taatggtgtg ctggcctgtg 60gggcgtgggc tcagctctta ggatggtggg ctgggcgccg acccagcaag gacagggtga 120tggcaggtcg tgggctcagc aaatgagtgc ccaggttgtg ggggtgggca cttggggctc 180aggggaagct catcagcttg gagagggacg ggggagggag ggggccttgg ccagctggcc 240cagatgcctg gatgtgagca ctcacgtgcc ccggggtcca cctcccctcc agtgccatct 300gggcaggagg ctccgatgcc tgtccctggg acccgctgtc ctgaaatgag gttcacttgg 360tgccttcccc agagatgctc ggtccggaag ctgacgaggc aggagtgcac aagggtctgg 420ggaaatggag cagagtgcgg ctggggcaca gaggctgccc ccagcctggg aagatgggga 480gctttgcagg ggtaccccgc cagcttgtgg ggccctggat acccaagggt gtgaagaggc 540tgaagagcga 55085984DNASus scrofamisc_feature(679)..(679)n is a, c, g, or t 85ctgagcccag ctatgtagat tagaccccgg tccgtcccaa attcttctca aagctgtccc 60gagatgagag atgaggtttt cgtgtcctgt gctctcctcg cttcccctgg gatgtgccct 120agggtgggag agggtgtgtc ccagggctca gcaggcggtc ccatcttccc gagacgggag 180agatcccctc cttctcggcg cctgtcccca cggcccccac agacaccccc ccccccggca 240tggcacccat gcacctgcca tcgtgcccag taggggatgg gtttggcgag actggagatg 300gctgtagcca gtgagacatg ccctgccacg tagcctgacc ccctgggtgt gctctgtgag 360atctggggac ccccagcaca cctagggatc atctttgcca gcctcctggg gagcctctca 420gaaatggggg cccccagaag gctggcaaag gtgatgggga gcgtgggaag tctggcggtt 480ggcggggtgg gtggggggca gtgcgggctg ggtggggggt gctccggggt cggaagtggt 540ccagcaaggt tttggacaca aagtcaggag gaaggagtga cgaggagact tgcagaatta 600caggtagaat caggaaccca catcgacgcc aattgatcta tccccccctt tgattgtttt 660ctcctggggc ttttttccnt tttttttttt tttttttttt ttaatccctc cttagctttt 720tacgcgctca acaccaaatt aaacgtactc cccaccccac gtaacagggg ggcggtgacc 780cgaaggacga ggagcacacg aagccaccat ccgtcacctt ggcggcacca gccgctgtcc 840tgccctccgc ccatttatcg cccttgaatt gatttttgtt ttgctctgtc cctgtcgctt 900gggtagagtg gaaaagggaa cctctgtggg ggtgccagcc actgggcccc ccaaagattt 960caggggaatg aaacggctgc cgcc 98486550DNASus scrofa 86tgcccctgac aaccctgccc tgttagccac actcgcgact aataaggcga gaggtcagcg 60ggcagcccca cggggagaaa gtgcctccgt gccccccacc cctggctctg atggcccagc 120ctggcacccc aaggtggcct cggccttcct acctccaagg tccaggcgca tgtccaagca 180ccagcagaag cttctccagg gttggtgcct gctcagggca gaaagcaggg gtgaggctcc 240ccaaagggcc actggcacca atgcccccag gcagccccag cgaaggggac agcccacccc 300cagcccgggg acgcaggcct gaggggacat ggggaaccca gagcagggcc aaggggagca 360gagcccctcc tccgggactt gaaatctttc ccggggggcc cagggagctg gggtctgcag 420agggcacttt caaaatacgg cccaccccca aattgccacg tgggccacag agcaaggagt 480cgctgccaaa gtggcctggc ttcagcgcag gaagttcccc tcctggggcc tcccctccta 540taggcacagg 55087500DNASus scrofa 87tgagccaggg cctggcccag ctaagcccct ggagccctcc cggcctgttt cctgcctccc 60atgctggcgg agctcggctt actgagcggg ggccaggcca gtgtgcgtgt ggaggtagat 120tccactcagc tggaggttga ggtgggcagg gggccgcaga ccctcaggcc agctctggcc 180ggccaggtcc ctgaagctcc cccggctggc ctccccgtcc ctgcctctgg ccttgtcctg 240gcccttgcct gacaagcttc tgtggctctg cctgcaggag agacactggc tcccccgctc 300tcggatgagg acggggcttt tctgcacaag tcctgcccca gaatgtttgg ggcgccagca 360gctgagccca gcacgtctcc ccctgcccct ggctggacac gaatcccggc atcgaggcgg 420gaagggggat ggagggatgg ggcctaccca cccctgctcc ccacccagaa tagctgggcg 480gcccccatgg gaggccgccc 50088913DNASus scrofa 88ctgttttcac gtcttctgag gacacaccca gaagaggggc tgcaggcgcc catggtgact 60ccatgtgttc actgctgagg cctctgcaga ccgtctcccg cagcagccgc acccgtttcc 120atgccaccaa cagcgtgcga ggccgcactg tccccacggc tgtgcaactg ttttgaatct 180gagttatata agcaacagac gctccttcaa acacactcac gtgcacacgt gcgcacaggc 240gcacagacac acacacggag taataggcct cccccccctc cctgagccca gagggggcct 300ggggccctgg agcctgtgct ttagggcctt ttaggaaagc tggtgcctcc cagaggggcc 360gccccgagcg ttggcttccc aagtccccac caaccctcga cagactcaaa cgttggtttc 420tttcgtgctt ttgcccaagg gatgggcccg aggtggccct gcctgaggtt tcagcccagc 480gccccaggca ccctttctct cccggtcccc ggccacttca tgggacagcg ggccttcccc 540cacgttgtcc cctgggttgt cgtgcttttc gtaatgagac ggaggcaggt gcacctgtcc 600tggggtgaat tctcttctgc aggaactcgc ttccccggcg cctggtctgt ctgttcctcg 660gttgttggaa cctctcgtca ccagaaaggg tggctctgac gtcgcccttt ccctccgtgg 720cttttgcagt ctgggtcttg tcggggaacc tgccccaaag aggggagtga ccccccacga 780gggagacgta gctcctgtgg cgacagcacc gggggccccc agattcatgg ggttcacgct 840cacagtcgca tgacgctgcc tttggacgag ggcagctcaa gggaagcttg tttcctgcca 900cgagccacag gca 91389650DNASus scrofa 89tccacacctg tggagccgct gcctcgctga tgccctctgc ccagctgatg gtcaggtgcc 60cagacttggg gctcagtcca aacaggggcc cacaggtgct gcacctgggc aagggagcct 120gtgcgcaggg cctcaggtgt cccaggctcg ctgggaccga agcgcactgg gtcctggact 180ccgggcttcc ccaggggctg ctcggggcca cctggaaatg aagccccacc tggctcatag 240ggtccacgtg agggccctga ggccaccaag ccaccaaaca actcagttaa gggaggggag 300cttggggctg ctaagctcca agcgggaagc ggccgcactc agcactgcct ctctgccagc 360cagccgccca gcttgctgac gtcccaacca ggccagggac cctgtcccac agatgctggg 420cccttccagt ctctgctccc tggaggcgct gggcactgtg tgggcacaca gcccgcaccc 480gcctgtaagg aagggaaagg ccccatcctc aaaaaagccg tgggcaggtg ggccatgatg 540gtcctccgag gcaggtcctc ctgggacccc ttgctccctc gggctcgccc aggagccgcc 600aggtctgccc tggattaact ctgccccgca tgtcattttc aaactggctt 65090700DNASus scrofa 90tggggccctt tggggccgga gcggccagtc tgctgggccc gggagcaggg ggtctctgtc 60cgcagggagg gggcctggtc tcaggggagg agaggaggca ggtctcacct gaaaggatct 120gccttctcct caggcctctg ggatgcctgg gcagagaaac cagaaggaaa ggcccaactt 180gctggctggt ggggatgggg ccgggggtcg ctcccggcac acccccccca aaccccacct 240tagtggccaa agtgggtgtc atgatggcca ctgacctcac gggggcgcag gagacaacaa 300aatttcagcc actcttgggg gaaggacact tgtggcctga gtcttagggg ctgagtttcg 360ggggggaccc ccagctctcc ccccagtatg agacaccctg cccactcctc ccagctgctc 420cccaaaccca gtgcttctgg acgggcatct ccccgctgcc cctgcagccg ctgtcctctg 480accatgtccc ctccccacct cccctctgca gggccaggcc tccagggagc agagccgagg 540ccccacccta gactgagctg gggaccgaga ccccaagtcg ccacccggtc tctgcgttag 600agagggggtt ccggggggca ccctggggcg gcactggggg gcgggaagga gagccctggg 660ccgttctggg aaaggtctgg gagggaggga ggggttttgc 700911400DNASus scrofa 91gcacacccgg agaacagagg gaggggtcct taccagtctc agggtttttt tggggatttc 60tttgaacttg ccctattggt ttcgaggctt ctgttctctc caatcccccc ttctgaaccc 120ccccaaaaat gggttcagcc cccaccccag ccagaggaaa ccaattgggg gattgggggg 180aggcggggcc agcaaaagcc ttgggccccc agcccccctg gctttggcct ctggcctgcc 240aggtaggggg agggacgcgg tgacctccgg gggcctggcc acggactctg cccccacccc 300cagggcagac gtgcacagga ggggagaggc tccgaggaat gaggccatca aagggacagg 360tgaggccacg agccgtggga cctggaagtg tttagggcct gggggacgag gctgcggcct 420gcgggctccg tggtcaggag gccctctgcc cactgagcag ctcccaccac tggcacacga 480gcctctctgg ggtccggctg gtctccggca ggggtgggct ctgaacgtcc agctccgcag 540acaaatcaga ttcccccgag ccctgagaaa gccccctccc ccagcccgtc tccccacctg 600tcggtggaca gagtgacccc tgctgacccc ctgcccgggc tcccgcagga gatgtgagag 660agtaagaggc ggtacaggac ggccggggcg gcccgggcga ggtgcaggtg tgtgggtgtg 720aggctgggca caggctggca cagcctccct ggcccagtcc cttgggcacc tctgggcacc 780tcggtgtgcc tgcctcctga agggatccac cctccagcca cctcctctcg ggccagcccc 840caccccaccc ccgagctaca gatgcctgcg cattcgcccc aagtgtcctg gaccctggag 900ccaggcagcc cacccgctca gcctggccag acccagcgtt gcccttcacg ccctcctccc 960tcccgccggg tcctcgcgct cgtctcctca ggttggaagc cccttcccac ctgccatctt 1020gcctgcgccc aggatacacg gctcaactca aggcctcact cctcgccctc tccaaggctc 1080tgtccaggcc cctctctgac ctggcaccac ctgccgcctc ctggcagccc cagcaaaccc 1140cctgccacag tccacgacag tcctcttctg gctctgcccc caggatgctt ctagaactgg 1200ggggggggtc cttccagccc acgcagcatc cactgggccc tgggctccct ccccaggtgc 1260ccctcagagc ttgcagctgg tgcagacggc tctgctccga acccatgctc cctgcgccct 1320tggacctggt gagatgttgc aggtcatttg gctgcaccca aaagagtggc ccctcagggt 1380cccccctgcg cccctccatc 140092391DNASus scrofa 92gtactgtagg gcctcattcg aatagcctac taggtcacag ctgatccaca ccttaggcca 60tcacaacttc ccagaggtag tgccgctcct gtcgttgaac aagacggtag tgactgctgt 120gagagctcag atctggtggg tcactgaccg agtgtggaac cctgggggaa ggctgtgggg 180tgtccccggc tgggtggcca tgtcatgtgc ccctttctat cccttggacg aggctggttc 240actcggctct agagccccaa gccccagctg ctctgccaac cccccaagcc tgagcctcat 300cagacccacc accccatcgc catggctacg caggacacac cgctctccac ccccaccagc 360cgccccacct ccccgaggtt ccaaagcttg a 391931464DNASus scrofa 93tccaggacct gatgcagcag ccacgtcgcg aggcccctcc cacgaggccc cttgttgacc 60agcgctaggg aaggggacca gggagatgct gagaacgggg ccttccgagg gggcaggtgg 120gactgactgt gacccaacac tccccacccc cctctcccgc tccagagggt gccagcctgg 180aagctggcaa agtccaatcc acaggtgggc tcacgtgggg aggctggtgg cccccacctg 240gtggggcccc aagctgcctc tgggcggggt gggggctgct cccagcaggg tcccatccag 300cttctccctg gggagactca cagttctggg agaagggtcc tgactgcacc gcagcgcccg 360ccccctcccc agactcaccc aagttctctc tctgcatcgg tgactggtct ccgcatttgc 420ccaggctggg catctgccca gaggatacgt ccaaaggcag ggcaaagccg ggcccgtccc 480ccggagctcc ccacaggcgc tgagggctgg gctggatctc gggggggtgg aggggaggac 540tcagaaggtg cagcggggtg gagcgaggct gagccaaggt gcacgcgagg gccagagaag 600gccgaggcgg gcaggaggag agagcgccag cctggagggg ggtgggtgcc ctgggcaggt 660ctggggctca agaagaagag agtgtgtgtg cagggggctg tccaagctgc ccgggaggct 720gcctgcccac ctccagggag caaagcaggg aggctgcagc tggcccggcc ggccgctctc 780caggaccacg cgtggcccag gcctcaacgc tcctcccaca gcccaggaga cccagggcac 840cgggtccatt taccgcgggc tccgggtccg tttgcctgcg ccctgggatg gactgtgggg 900gcggggcgct gtctggggag gagggaggtg tctgaggctg gacaccttga aggcaggtga 960gagtgacagg tccgtgcgca ggagccttcg gctctggatt ctggccctga gcgaggggct 1020ggctggaaac tgggccgggg ctgccgcagg agagtgtgca gggagaggag acggggtttg 1080gccccggagg tgccggggtg gtgccctgga gtgcggctga gcgggaagtg ggtgttggcg 1140tctggagacg gggggtcgtg ggcttgggat ggtgacaaga ccccccaggt ggaggcggcc 1200gcagaggagg cagagaagcc aggccccagc cccacggcgg gaggcctggg agtcaggagg 1260gaccagcaga gccctgggct cagtgtcacc ggtcctggca cctcgccgac ggatgtcctg 1320gccgtgcagt ggttgtcccc tcaccctgag ccctgagaac catgcaggat gctggtgtca 1380cagcaggaga gggccagggc ctggggagga gtcttactgg aaggccttct ccttccgttt 1440gcagcaggcg ggaatgactg gggg 146494694DNASus scrofa 94tggagccagg gcacggcaga gcggtcccga ggccgtgcgt gctgacccgg gggatgggcg 60gacctggggg tgggctgtga gcccaggcat agggaccccg acttgggcac ggccaggtgg 120ggccgggcaa gggggaacaa ggacgctggc ctccaagggc cccacgtggg cacagaggaa 180gagccgaccc aggttgtggg cgcatggaac cccccactct gggggccagg aggccgaacg 240tcccaagggc tgaggctggg agggaagagt ccctttgggg gtcagtcagt gtcccttgtg 300ggtgcccccc tgccactggc ggcacctctg accccaactc cttgcgggtg gacggtggat 360ggatttcctg cagcctttct tctggaatag tctctgccat cctcggggaa gcagtgattg 420ctctgcccaa gtccaggccc cgccctgcaa ggtgcctccc accccaatga gcccccggac 480agttcgaggg cttctcacgc tactgagggg tatgaacagc tgtccccctc ggaaagtggg 540ggacaggccc ctgccactcc atcctcggga cgcccggtct agtcagcact tgtctccctg 600ccttgtgccc ccctgacctt ttttgaggac catcaaaacc tcagcctctg ccccaggagg 660tcaagccccc cgtcccccag cccccagacc agca 69495900DNASus scrofa 95ccagccccat cccccggctg gtcccccacc acacagagcc cccgtttccc aggggacagc 60acagcctgcc cccaggtctt acataaagtc accttctcag agctcctgtc gcggctcagg 120ggaatgaatc tgaccagcat ccatgaggac acaggtttga tcccaggccc cgctcagcag 180gttaaggatc tggcgttgcc gtgagctgtg gtggaggtcg caagacgtgg ctcagatctg 240gtgtggctgt gactgaggtg gcggccagca gctgcagctc tgattggacc cctagcctgg 300gaacctccat atgccgcggg tgcagccctg aaaggacaaa aataaataaa taaataaaag 360aagtaaacac accttctcta gccataacca cctgcctagg ggcggagggc caggaagcgg 420caccccccgc cccaggctgc ccgtgcgccc cgggcaggcg gctcagcctg ctttttgtct 480gtgatgtgag ccgccccagc cccacatgga ggggctgggc tgcgcagtaa ctgctttaac 540tgacgggagc ttcgaccagc aattcaccag cgggcatgca gccgggaagg gaagttattc 600gtgtgtagct attaggcgcc ggagtgaggg tgtgcctcgc cctgggccca cccctggggg 660gaggcatcac aggggttttg aacacctgcc catgaacacg gggcaaaagc cagccaaggg 720ggcaggtgcc tgaggctggg aaccaacccg tgtctctgaa atccggggaa tgcccactgc 780aggcatgttc aaagggtcaa gaccggggct ctgcctgaga aggactggcg aaggccaact 840acaaaagcgc acccctctgt gcaaaccccc aaccaatgga acaaaactcc agaggggcca 90096550DNASus scrofa 96agtctgggct gtgtccatgg ggttgccaag gtgccaggca gagaccttgg ggacaaaggt 60cctgtgagca gaaggacatg gccacgtccc ctgctcagca ggtgcccagg ctggggtctg 120atgccctcgc tggggtgggg gcgggttgag gggccaggcc cagacaccct tcgtccctgc 180cggagttgtt tgcccttctg ttcctggaag gcccccctgc aggtacagga ggcccctggg 240gctgacgctg caccttctga cacctgtggt cttggggatg ggacaggaca gggagacccc 300ggggctggac ggagcgggta agacagagag ttgactctgt cctcgagtct gtgcagggct 360gtccccggct tgggcttcgt ctgcagggcc tttcgggtca gggtggcctc aaggtgacga 420agacctggtc ctcgggagtc tgcaggcgca aaagttggag cccacccccc cggggagggg 480gcgccaagga caggagggcc cagggaagtc tggggcctgc aaggccgtcc gggctgggga 540aggccaaggt 550971200DNASus scrofa 97gtttgctctc agcaggcaag ggcctccgag gccttaatag cccataatga cagcgcccgc 60tcctggcatg gggccccgcc tggcatgggg cagggcaggg cagagcaagc agcatgcagc 120ttctaccttc ttcctgacct cgtggcccct tccgaggcct cagggggtcc cccgagtggg 180accccagccc tggctctcct ctccagagcc aggcccaagg ctgggagtgg cccagagatg 240agggtgcccg agcagggcac tgccttggcg tccccatccc tggcgcctca gggccgtact 300gtccaaaacc aaaagaaagc agtcagcaaa acttctccca gcaagctggg gtcaaaggtc 360gcttccgagg cgtgatcagg gtggcctttg ctactgtcac cgtgtgccct gggagaggca 420cagggacaca gacacacacc tccgagaacc tggggcttcc agggcgtcag gctgcctggg 480ccatcccggg cccctgtggt cccaggatct gccgggaccg tgaggcctgc gtcccaccct 540ctgcctggga caggccccac agagctcaca gccaggggac cggggacagg gccccgcctg 600ggccacctgc ctccagcctc acccagcctg ggccccaggc ctgtgcctgc gacaccctga 660gtctcaggac gggcgcggga caaagccgcc cggcccctcc cccggctggg aggagacccg 720cgtggccctg acgtgtgggc ctgtcagagc tgaaatgtca cagcaattag ccctaacgag 780gccgagggag ggagcggcgg ggaggccggc ggaggggatc cacgagccga gggcccggag 840ctggccaccc caccggtcga ttccaggcac tcagggataa ttgggtgttt agaagtcagg 900cggcagcaga gagcgggcca ggcgggctgt gccccccctc ccaccgcccc ttaacaggtg 960cccgaacacg caggtctggg gagatgctga ggtcgccaag ggcacccctg gccgtgccgc 1020gggtgctatg ctggttcggc accatgggag ctgcacctgc agctgtattg gtctgtgtgt 1080gtgtgtgtgt gcacgcgtgt gcgtgtgtac gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 1140gtgtacgtgg gggggggggg caagcccgtg cgtgtggtgc acagtagaca tttagaaggt 120098600DNASus scrofa 98ggggaccagg gcccagccct ccagctccca cgcatacctg ctaggagctt gcaacctgcg 60agagctttgt ggaccccctg ccgggtgacc cctgaagctg gcagctctcc ttggctctgc 120agcggctctc tacactaccc cctctccagc ggcctcgggc ccagacatca cccacccgca 180agggaagcag caagcatcca ccagctgggc ccttttcccc cagcctgtga ccggccccgc 240gccccctcac acctctgcgg tccaagaccc ctctctggct gggccctggt gctgcccttg 300ccgtgcacat ctggggtcca taccccacca acaggcccca cttttctgtc tcccagtgtc 360cccctcagct gccctgatgg gcccacacct ggcttctctg ctgcccccct tgaccgcaaa 420aagactgggg tccaggaccc cctgccccat gactgccctg gaagacctca agcctctcct 480ctcaatcctg accctttaag gctcttgcca cggagaaagc ggctggggtt gggggagggt 540gtgggtccca aagcagcttg catacttctc ctgactggga gctcattcct ccacagcgtg 600991350DNASus scrofa 99cccgccttat ttttaaattt ccgaaaacaa aaaccacacc tctcccgtcc ccgaaattat 60tttggtatag tcttattcaa agaagtcctg ccactgaagc ccacttgtcc tgtcccgggc 120tgctttggcc aagggccctg acgggcccag ggtggctcat tcccgcatcc ccgcagaggc 180cgccttcaca tcccatgcgg gagcctggct tccggcaccc ggctgtgccc tcgctgtggc 240catggactgc tttcgcagaa gcataggggc cacaacatgg gacagcctcg ctctgctcgc 300tgtggttccg ctgaacctct cagctggaca tctgggcagc aagcacccca gctttgcttc 360aggctctggt tccaggctgg gccctcctcg gccctgcccg ctgggtgcca agcagggctg 420gtccggctgt gcccccgggt ctatagaagc ctctgcaggg cttcctacag ccaggctggg 480attcggcggc tgcccgggac tgaggccccc tctgagtctg acccccccat ccttccctcc 540cacacagccc cccgcccccg cttctgcttc agtgaggccc caccctgcct cactcgctga 600catttccaga acagggggtt ccaggaagcc ctgagcctgc aggggactca gtgaccagcc 660gcatctgaat tttccctcct tctgatctct ggagacacgt ctggctcagc ctggctcgag 720tgccctgagc tggggaccag gacagacctg cagatggagg tctgagcctg ggcagggcag 780ggcccaaggc tcagggagaa attgcaggtg tgagatcaat gaccggagcc tggatggggc 840cgccctggcc agggcagctt tctccctgca gctccctgcc actgtccccc ccaactctgg 900gctcctgctc tggacccagt tgtgtgttcc cctcctccca gccgagccac cctcccccat 960tctgcccccc ccaatccaac accctatcgt gggaaccagt ggagctgaaa gaaggacccc 1020ccaagggccc cccagccgct gtaatccttg ggggcctctg cccaggtgcc aggtctcggg 1080caggaggggc cgcgggcaca gccgtggcag atgcgccccc caagcctggg ctcggaggag 1140ccccgccccc actgacattt ccaggccgcc cgctgcagac ccggctggcc gtgatattta 1200gacagggctt atttgccgtg actggttttt gatgactttg gggcccagga tgagctcagc 1260cgagcccgcg ttggcccacc ttggtctcag cttgggtttg ataatataac gcgttcaact 1320gaaccgctga cgcctgcgtg ggccgaggcc

13501001354DNASus scrofa 100gcttgcagta gttcatcaga ttggacgact cataaatgtc aagacatcta aagattggtg 60catccaatca tttcccacca ggttgttttt ttgtagatgt caagaagctg acccaaaaac 120tcacgtggaa atgcacgtca actgggagag ttgaaacaat ttctaaaaag aagaaggacg 180tcgtgggagg actcttcgcg ctctttggtt tcgcttcact ttatattatt agttactgat 240tttcctaaaa gctgcagtag tccagacagt gggcctctat gaagggaggg gctcagagat 300ggttgggaca gaatagaaag cccagaaacg gacccccgca aatgtggtca attgagtttg 360ggcaaggatg tgaaagcggt tcagtggaga agagtctttt caagaaatct ctggtcctgg 420atccactgct catccaggcc caagagtgaa cttggcgcac atttctcaca gtgtatacaa 480aaactgactc aaaataattc acataccgtc gtgtagcgta tgaagccatg aaacatccag 540aagaaaatct cggtaacctc agggcatctg gggcctccac cctcagcacc actggccttg 600gggccagata cttacgtgtt ctcctgtgca ctgtgggacg tgcagccaaa ccccaacaag 660gtgaccatca gaaatgtctc cagacgtcgc caaataactg ccagagagca caggagcccc 720tcactgagaa ccacagggtg gggcagagag atctcagaca tgacacgatt aggggaaaac 780aatctgacac actggctttg ttaaatttaa aacttttccc ctgtaaaagg caatggtaag 840acattaagag gcgaagtggc agactgggag aaaatatttg caaatcatgt atcagatacg 900aagaagatgc aggaaatcct caaagttcag tcacaagaaa acccaattca aaaaccagca 960gagcagacat acgatggcaa ataaccacga gaaagtcagc acccgctgtc cctgggggga 1020cgcgagtcaa agccaggagg acaccaggat atgcccactg ccaaggctac ggataacggg 1080aagcaagaga cacagacaga aaggatgctt cggtgctggg gagggtgggg tggggcgggg 1140ggtccccccc tggagcagga tgtgaaggca cttggggggg gctctgcact cctgggggcc 1200tttggcacag gcggagggcc cgggaaggct ctaggggcac ggagaggggt gccaggcttc 1260cttacccagc ccaggcagac caggccctgt catgaagcct gacgtgcagc agcaagagca 1320acatgctaca gacatgtgtc tgtgtgtgtg tgtg 13541011000DNASus scrofa 101ggttctcagg cgcacggggc agaggctgag ggtccgaggg gctttgggtg ctggaaagcc 60tgagtttgaa tcccagctcg gtttcttaaa gctgtgtctc cacggccaag gaatggggcc 120tctctgggaa aggtctgggg tgaggctggc gggacctgcc agccccggag ggcatctgac 180cagacagctt ctcaagctca cagggcttca tggcaggatg gggaaggctg tggtggggag 240tggggagcac tcgacaccct gtccaggcct cttgagtcac ggtggcctct gaaaaggggt 300tctctgtgtc caatgagcaa gtctttgtcc ggggcaggat tactaagtcc aagggtgtct 360gcccctccgt ggggcacaga gcaggggccc cagatcacgt ggctgtaact gccaggttgc 420aaagcctgcc accatgtccc actgggttct ccagttacct tgggaggtgc agggtggggt 480gatggggaaa ctgaggcaga gagctggcaa aagagtgccg gcagggactg cgggcgccag 540acccagctaa ccgaccctca cacggagctg cttctacttt gcagcctgga cgtgggaaaa 600ggttacccca cagcagcgtg tgcaggcacg ctggtatgtc tgtgtactta tgcatatgtt 660ctacgtgcat gcacgtgagt gtgctgtgtg cattgtgcct gtgtgtgtgt gcatgtgtgt 720gtgcactcat gtgtctatac gtgtgtgtag tgaatgcttg tgcatgtgta tttgcatgtg 780tatgtttgta cgtgtgcagt gaatgcatgt gtgtgcagtg gcggcatgtg cgtgtgtgcg 840catgtgtctg tttatacctg tgtgtagtga atgcatgtgc atgtgtgtgt ttacatgtgc 900acgtgagaat gtgcactcgt gcatgtttgc atgtgagttt catgtacaca tgcttttaac 960gtgtgcacgt gtgcacatgt gtttctgtgt cccttgcacg 10001021500DNASus scrofa 102cgtataaata tattaatata gaataaaata gattgataat atagataaac taaacccatt 60atcaataccg ggtggcccca gcaaaggata ctagccagtt tatcaaggtg ctaagtcagc 120acatagaatg gccacaaacg aaaacctgta ctgcctatgt ccactctaat ggagtatgcc 180actgacatca gtggtaggtg agctgagtcc atctgggctc ccagttcggg cccggcttgt 240cccccaacgg aggttccttc cagggttccc caaacccaac cgggccccca ggtctccctg 300tcttgactcg tttctggagt cttctggggc tctgcagtcc tcccttgttg gggcttctgt 360ccccctgccc ctggccttgc gggctcggcc ctgccctggg tcccgggcct gcgggctcac 420cctccttctt tccctggaag agagggagcc aggctgggcc gggccaggag ggaatgcgcc 480tgactctgct ccagatggac aggtcgggac atgcagtggc ctcgccttgg gctgctgagc 540caagagcagg acgggttctt tctggaatgt ggggccagcc aggttcagcg tgtgggtggg 600cagccgccag catctgtcag ggccgctgca ggcgcgggga atgacctcga cttctgcttg 660gcacccagct ctggaacagc cccctgcgga gcctccgccc agagctgggc cagagggtcc 720cctgtgccgg ggaccccagc agggcccctc cctgactctc caacccacct gcctgggagg 780agtggccccc tggcctccgt ggatctctgg gtcggggctc agccggcttg acagcctggg 840aacagccaat gcacatcccc aggcctggcc acacccttcc accgggagcg ggcggatctg 900catttcgcca ggctctgcgg gcagctctga gagccccggg tctcggagcc cagccgtggc 960cgttgtacgc cctgggggct gtggacagcg tgtcctcatt gcccctccga ggtccggccc 1020aggtcccctc ccacctgctc gcccagagcc ctctccccac caaccacact tcctgctgtt 1080ctgcaagcgg gacacacact ccggtttcag gacctttgca cgtgccgctt cctctgcaga 1140gaaatgcctg gagcagatgt ttgtccgcac ggctgctccg cgaggcctac cgagagcccc 1200tcacctaaac ggccgggcct cagcagcccg gggccctgtc cccaccgccc aggtggtggg 1260ttctcctgtg ccagtgtggg catctctgta agatacctgt ttatctgctc atcgtctggt 1320ctcccccaga aggtagagca gggcccggca cagccgtcct cggggtggcc actcgccctt 1380ggggctcagc ctccatgcag ggagggacgc ctggtgacac gagagccccg tgtgagtgtg 1440ccgggccgcc agcctgcctt aggtcacagc caaagccggc attaaccacc aggccctcga 1500103600DNASus scrofa 103tctagaatac ctggccctcc agggacgtgt cctgtagctg cggctttcag ggcaaagtgt 60aattaaacat ccccaggctt cccttccagt tggcacaggg cacccacatg aggagcagcc 120tctgggtgcc aaagggccca ctggtgccag gcgctgggct gagtgcaccc ccgcatgctt 180cccgcccact cacctgctgg ccccacccct gaccacagca cctgtgggaa cactaggcct 240ggcagccaca cgctgctctc actggaggcc agtgccaggc agcctgcttg gctacgctag 300cagatgcccg ctcgcctctg cccctgcccc tagcccatgc aggagcccag ggtggggcac 360aggaaggacg attggggccc caggtcaggc acatccaggc cacagccgtg gccacacgaa 420ggcggccctg agggggcgtt ggggggcaga ccctgccccc ccgctgccgc cccagctcca 480ggcattaatt cccagggacc tgttgcactg ggtggccgcc agcctgcccc cttgccttcc 540aaggcctcta aaatgcccct cttttcgtaa actaggactt accaagctca gcgagccctc 6001041867DNASus scrofa 104agtatatcgg gtgagactgg ggaccggtct gccgggaagc cccaccataa aggccacgtt 60gggccacagt ccgggccacg tgagtgtggg cgggtccgcg ggtctgctct tggaacacca 120ggatctctaa gaggtaccag ccgaggccaa gttcacgtga gcaagtgagc aaatgactga 180atgagagcgt gagcgaatga gtgaggggtg agtccgtcca ccacgcagcc taggctcagc 240caaccgctgt ccccgcgtct ccactggtga ccagaacgga aagagtgggg aaagagtggt 300tgtctcccac aacccagtcc ccaacccccc tggacgcccc acccctccag gggtgccggg 360cctggcctgt gggccccagt ctggaggctc tggcaccttc ctcatccgtt ctcccagcac 420cccaggttcg tgctgagccc tcctggccca caggcctcgg ggacaaagag ggccacctgg 480aggctcaggg agcctcacct gcctcgtggt cctggcggag gcgggtctgg acatgtgata 540gaccggcctg ggctcagcag ctcctgctgg aagatgtcag ggacagcctg ggccactctc 600ccaccaggag aacttattcc tcggtggggt ccccccgggg aagggatggg atcccagcgg 660ggaccccaga gcgtccagca cacggacctg tccctccagc ccctgcccca cacggatgct 720cacagctcag cctcgaacac gcacctgttg gactttgcct cctgaggctg tcttctcagc 780cgacgcgggc ctccgctgca tggtctggaa gcccagtggg actcggtggt gacagggaac 840aggggctctt ggagtggggt gccgggggag ccccgaggga gctgcttggg cctttgatgg 900ctgagtgggc tgaagtcagg caggctcccc cagggctccc tgaccccccc cacctcaaaa 960aatccagagc atcctttgct ttgggtctgg tgaggctctc tgaggtcaga ccctgcgtgg 1020ctgggccagt ggggctggag caggaagaaa gcaggacagc ccccgcccct ggcccagact 1080ccccaaaccc agcaggagac acctgaaacg ggatggaacc atcctgaaaa gagccacctc 1140ctcctcctta tgcatcagct gccggggtct gggggcccgc cccaggcccc agatgtccgg 1200gctgctcccg tctcacatcc aggggtttct gggcccagga ctctgtcccc ccaagcatgc 1260agagggtcca ggctggggtc ttcatgcctg cccgtgtgca tggtggggaa ggaaggggac 1320agtctggaga ccccccgccc tccccatgcg tggcgccggg ggacaaagcc ggctggggtc 1380tcaggtttgg gttcagagca aacgttgatc tgacctggtt ctgagatgct cggcccgatg 1440ctgcgttgtc cgctcgcatt ttcctgtttt ctctgggagg cgctgcgtgc gctgtggctt 1500ccggccagcc ccacggaggg acgcagggtg gctggcgggg tctgggggcc cctgcccgca 1560ccagaacgtc tggctcaggt ttttgtcctc gtgacccatc actaagggcc accctctgac 1620ccggagccct gtctccgagg tgggaattgg gggctgtccc tggcgtcata ggacctggtt 1680gggggcatcc agggctgtgt catgcccctc cccagaagac tctgggggct gcgggagggt 1740ttccccagct tcgggccagc ctggggaggg cggaaggcgc tggaggcctt gcctgtccca 1800gggagcatgg cttcgctgca gactggggcc ccgcacaccc agccaccact ggccgtctgg 1860aagcact 1867105650DNASus scrofa 105gttgaggatt cctcggcaat ttcctcgtca ctggcgctcc aatcgcctcg atgggcttct 60cctccagata cagctgcaga tcctgggcgg gcacaccgtt gagcgtcacc tcgtagtgca 120gattgcactc gttgtcaatg gacatccagg ccatgccgac ggcatgtgga ttctgtgcat 180ccgtgtgctc ctgtcgcttc agcagaatgg gttccgccga gtcccgagca tcggccactg 240gacggggcac taggcggcca cggatcaggc tcgtctcatg ctcggtggcc acattaacgc 300ccagttcgcc ggcatacagc gactcgagga ccttgggacc caacttctcc acactaccaa 360tggcctggtt gaagttgaag ctcggcgtca gatcctccag cttggccttc cgcttgccct 420gctcctcaat caaactgatg ttgggcctat cccgggtgtt cacgtgctcc gtttcgatgt 480tgtaggccag agatccatcg gtgttcaagt agacccacgc caaaccgctg ctcttggtcg 540aggattcggc actgtgcggc gccagcaggg tctggaagat ttcgcagctg gctcgggtca 600cgatgtgtcc ctggatgcgc agatgtgggt acttcttgga ctccacggtc 6501061630DNASus scrofa 106ggtgttgtca ctgctgtggc tcagacccct gctgtggcac agggtccatc cttagcccag 60aaacttgcac atgccacagg tgcagccaaa agaaaattct tactaataag ttgttcattt 120gcctttacgt agagtggcat caaacagcaa atttaaaaca ccatctatca atacatagac 180cgcggtcaaa gggaaagaac tttctatttc agcaccttta acatggcttt gcccgaattt 240gggaccaggg tgctgtgttt tcatctctcc ctgcaggtgg tccccagatg accaggccgg 300tcctgggcgg gaggagccgg actgtggatc cagttgcttc ccaagacagg ctgacaggag 360agcagcaagg gccaccccca accgaaacca aagccagaac gagcagaaag atgccgtctt 420ccaagtgggg gctgggagct tcctcccatc ctccggagcc gtgaggctgc cctggagctg 480gcaggagcca cagaggaccc ggctttgacc gcccctctgg gacccacaat caggaccctg 540actcagatgc tgaggggcct ggacaacacc ccaggaccct gctgcttccc cagaaccgct 600gtgtccatca aggtccagat ggcacccgtg tccccactgg agcacgcact ccgtggggca 660ggctttccct tgggcaccga tgcaccttga gggcagagac ggggcccaat aaacgtttcc 720aaaccagtgg gtgagggacc cgaccggccc gacacggcag cccggatgca gggactccgt 780gcttggccca gcctcccttg gggtggtcct gtgtcctcag gggtggatag gccatcatgt 840gggtggcctc tggggacatc cgttctctga ttgggtgagt ttcagccaca gagatattcc 900caggactaca aagctgggtc ccttggggca cctgctgtca caaaaagaca aggccctgac 960ccccagtagc caagttcccc caggggctcc ccagggtctg gtcatccaga ctgtgccagc 1020cgtgctgccc gccccagtcc tgcctgaccc gagtctctgt aaacatcccc cggccccacc 1080cagctttacc ccaaggccga aagcaccagc ccccctgcac cacagatgag gcccccatgg 1140ctccccgacc taacttctgt ctgcagttgg ctttcagcct cgggtggggg caaggcctgc 1200atctcaggct cccgggagaa gttgctgcct ccacagcaga gccaggggcc tgctgaccac 1260ctgggccggg tcggatctgg tctagaatgc tgctaaggtg tccttgcagg cagccccggg 1320cggccccgcc ctccaggaag gaaggggaca ttgccaggac tcaggaatga agccatccca 1380ggttttgaat ccccggtccc accaccttcc acctctgacc tcaggcacct cggctttcag 1440agctgccctt tctgactctg ggacacgggg ctgtgaggcg ctctcggtgt gtgacagctg 1500ggggggggca ctctctaacg agggtgggcg tgcccaggtg actgaccaca gccctttcct 1560ctctcaaaaa cgcccgcccg agtgacctca cgggaggcag ggccaggaac cccaaaccaa 1620accagaatca 16301071820DNASus scrofa 107agtgagccct gcaggacagt ctgctgaggg gtgtctgggc tcctcagagg ctcatggcca 60cgggcactgg gaggatagca ggtggacccc tgcatccagg tcccaggtcc caggtcccag 120acccccggac aggctttcta tctgcaggag gggggctcct ggggcagcag ggatgtggct 180gtgaggcctc gtcagtctcc ctgtttctat ctctctctgt atcacacaca cacacacaca 240cacacacaca cacacacaca cgcacgcacg cacacacaca gaggcgtgac cagggctgca 300gacagggcca tgggaggact gcccggcagt gcacccagat ggccacacgg tggggccctc 360gtcccacttt tgctgctgat gcttccgccc aggctgctgg gagcaagcac tagcttccca 420gggctctgac cagagaggga tgggaggggt catgggtcaa caggcgccag ggaatgggga 480ataggatctg aggggcgggg gcaaggggcc caggcgaggc tgcagtgccc agagctccct 540gcacctgcag gaccagccac aggccaacag ctgcaggcag agcagggctg ctcctgtccc 600cagaagctgg cacagcacat ggggtctgac agccccaccc cgggcctccc acagaggggc 660gggtccccca aactcctccc ccgtcccacc tcacagctca gcatctccac tgcctgagga 720cgagcccaac acacgggcac acacacacat gcacgcacac acatgaatgc acctgcaagc 780acacactcac acgtaagcag gtacacacat gcatgcacac aatgaacaca catgcacgca 840cacacgcatg cacacacgca cacacactca aacacgtaca tgcaagcaca tgctggtcct 900ttgtccccgt ggaggggagg atggaggccc agcccgtggg gagggcatgt ggagtgttgg 960ggggctggct ccaacgccct cgctcaacag gcaccaacgc tggactgaga taagccgggg 1020cgctggctcc cttggggccg ctcagcaggt ttgacgccca ccacaggtgg cactgcctct 1080ttcagaagac ggatgtggcc atgccaccct cacagcctca ccagtccccc ctcagcttta 1140gtggtgtccc tgtcactgta cccggggcct tccttcttcc agggccaaaa gcgagttcag 1200gggacagtgg cgcccccata attactcacc cagggtgctg tcctctgtgg tggccttgag 1260gccaaggtgc tcccatgggg gcccacaggg ctggcagggt cacttcctga gagcacccag 1320ggccaggggg gtggcccagg cctggccggt ccccatctgg aatgagggcc ttgcgcagag 1380gcggtgcacc cctctttaca gcagccccgg gggagagtga ctcctgcgtc atggacctgg 1440gggctgacct gtcacgtgtc tcgcccagtt gcaccccatc catttccggg tggaagggac 1500aaagccatcc tggtcgtctc agaggacctc tggagcctct tggccccagc agcccagccc 1560ctcccgggcc cgcatcctct gcccacccaa aatcacctgt gcccacaggg tccccttctg 1620ggtgtccagg gcgacccaga actgcccctg cagacacacc cagcccagga catggccgcc 1680ttgccgggcc tgtctgcctg gggcagcctg actgccacag acaggccgct tggaggacca 1740tctgcctgag cccccaaggc acatcccacg gggcccacac agccagcgcc tgtagacgat 1800gccacttggg gtggggggag 18201081500DNASus scrofa 108tgccgaatag aggtggaaac caagacccga aaaaatgtcc acatttttca attattagaa 60atttagaaaa atattttaca ggagttaaaa ggtattccat tctgggggcg ggtgggcatg 120cccacggcat gcaggcattc cccgaccagc gactgaactc gagccacggc agtcaccatg 180ctggatcctt aacctgctga gcccctgggc aactccagac actccatatt catgtaaact 240attttttaac caaaaaaatg acaaagcttt tcaaaacaaa acacatttca tgggaagagt 300ggcattgctt cacgcctgga tggtcgctgc ggcttgcggg acgacgaggg cccccgcggg 360agcgcctccg cacggcgcat caggacgtgg tgtccaggga agcggggtca cttcacggcc 420tctcgggtgc gcgtgggttt ccttttcggc accacacccg gactcagcac ttgggggttc 480ttaaacgtga gaggcactgc ggggctcgaa gccacatcac tgacctcctc agactctgtt 540atgtgaaaac ccatccgtcc acgagaccaa agagacagac gaacaaacgc aaggtggcgc 600ctaggttggg cacagcatga gggcagagcg gaaaccttgg cgaaatcccg gcgaagcctg 660gacgtcgcca gctcttactt gacgcaaaca tagggggatt caggaactct ctttaccgca 720tttgcaatta atttgctgca aatctaaaat cgttccaagc acaatgctca ctgcatggaa 780aaacccaggg gtaggtctcg cccgatcagg atgttttccc gtgccctctg tgcgggtgct 840gccccctgcg ctggtcagtg agaagtgtcc ctccaccgac gacatgaaac ttcccaggtc 900cacgctctct gctgtcctgg acgaaaactc atctctgtga atctcccgcc agctccgcgg 960gagccttcca gggctggaag gacggccgtc ccgttccagg gggcaggtgc acgcttccca 1020aagctccgcg tcctgctagg acgctcagac ggcatcaccc acaaacccca cgaactgttt 1080ccctcgaggc gacaggctcg cccttctccg agaaagcagc ccgcacacgt cagcaagggg 1140ccagctgcgt ttgtaactca aatggccaca tagagtttgt cctggaggca cggggtctgt 1200ctgggccgca ccactgcaca cgcagaatat gctgggacac gctccggggt ccagcttcat 1260ggaattaata aagtttactg cttcaccaag tacattctta agtgtagctg gccgccagcc 1320tgggcgtccg ctccgaggct gcctctctgc ctggaaccct tgtgctgggg gaccctctct 1380ccagccccac cccagccccg agcccaggca acatccttct tgtaagacac ccgctaccct 1440gccctcccgc ttctccttct ctggatccaa tctcctccgc ttctaagctc tcttgaggct 1500109550DNASus scrofa 109atggcactcg cggttgtgac tgagctaccg gacggcgcga gcagggccac gagggcgaca 60agcgcggggc tgagaacctg tgcgagggca ggtccctgcg gctgcagaca agcctctatc 120gcaggcccac agacaggagc ccccgtgtga ccctcaggct gcgagaccaa agtcacggct 180ctgctgggaa aacctcgaac ctgatgactg ggtgggtgac cccaggacct tgaattccgg 240cctctgcaga acgctctgag cctacgggag tggccaccct ctcggttagg gcctgtgtcc 300ttccctggct tccagcctag agcaaaagca ttaaatcaca gtgtggccca gcccggaccg 360tgcaggacct tagacaaaag aggagggaga gagagatgag gcagagaggc agagagacag 420aggtggagag acagatagac agagacagag gcagagagag agacagacag acagagacag 480aggcggagag acagacagag acagaggtgg agagacaggc agacagagac agaggccgag 540agagagacag 550110900DNASus scrofa 110tttctaaact ctcttactag ttctagtttt ctattgtttt ctgggggggt tctatataaa 60cattcgtgtc gtgattggag atggttttgt tttttcctct ccaaactgta tgccatgtgt 120ttctttttct tgtcttatca cactggctag gacttccagt aaaacactag atatgaacaa 180tgagaggaga gccaggcctt cttctcagtc ttggaggaaa cagtcagtct ttcctcattt 240agaatgagag cttttctttt cttttctttc tttctttctt tttttttttt ttaataggtt 300aaggaacttc tcttgtattc ttattttttt agagttgtta tttttttttt ctctcttttt 360agggctgcac ccgaggcata tggaggttct aaggctgggg tcgaattgga gctacagtcg 420atggcctacg ccacagcaat gtgagatctg agccacatct gcgacctata ccacagctca 480cagcaatgtc agatggttaa cccactgaac aaggccaggg attgagcccg catcctcatg 540gatgccagtc agtttcgtga ccgctgagcc atgaagggaa cttccaataa tgcaccaatt 600ttaaatgaaa aagacaaagc atccagccca cagcctgagt aaggagtttg gaggcctgac 660ccctgcgtgg tcctgggcct gggcctgggc tggtcggggt gggggggggt gggggggacc 720ctgtggaccc tccctcctca gccaggcctg cccctccatc cctagctgtc gggggctcgg 780aggaaggcgg gtggatgacg gtccctggga cccctcctca tatgtatctg ggtccctggt 840ccctctgagg cccaggtcag gtcatgggag tcaaaggtca gccaaggggg tagcccagag 900111950DNASus scrofa 111taacccactg accgaggcca gggatcaaac ctgcaacctc atgcttccta gtcggttcgg 60taaccactgc gccacaacgg gaactccttt gcttttgttt ttaggatttc acatacacgt 120gataacgtgc cgtatttatc tttctcatct gaattatttc acttagccta agcccttcag 180ggtccatcca tggtgctggg agtggcagga tttgcttctt tttttttttt tttttgtggc 240tgaaaatcag tccaggatta tcttcttttt ctgttcatct gtggaggaca caggctgcgt 300ccgtgtgacg ctctgccggg aatacggggg ccgatcgctt tctgagccag tgttctcatt 360ttcttgggag aagtacccgg agtggaacgg ctgggtcgtc ctgcagttct gtgctgcatt 420ttttgaagac gctcggagcg ctttccacag tggctgcacc gactgacatt cccaccgaag 480tgcacggatt tccccatcct ttttccacgt tttccccgca cttgctattt ttgccctgtg 540gatgtcggcc tctccgtcag gtgtgagggg agtctccgtg cggcccaggc gaggagcgac 600cgtgagcgtc gtttcacgtt cctgttgggc cacctgcgtg gcttctccgg aaaaagggct 660gttcaggctt cttgcccatt tctcagtctg attgtttggg gggtttgctg ttgagttgtg 720tgagttccgc acgtatgggg ggcatcaacc ctttatcagc tatgcgattg gcaagtccgt 780tctcccatgt tccgccggcc gccttggcac gtgtgggcgg tctccttggc tcttccttgg 840tgcagaaggc ttcggtctga tgtgggccca tttgtttatc ttcttttctt tcctcaccgt 900tgttttgatg tcagatgcaa aaatccattg ccagggtctg tgccgagaac 950112306DNASus scrofa 112cgccacctca atcgccggtt tgttctgcaa cacggtccag ataaccagcg cacctaacag 60gtcgaacact gccagaactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc 120gacaaccagc gcaaacagcg tacttgccag ccatgcggac atcccggtta aaccgtttgc 180cgttgccact tcgttacgac

caaacacatc ggaagagagc gtaatcagcg cgccagacag 240tgcctggtgg gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa 300caggcc 306113800DNASus scrofa 113gttttccatg atgcaccagg ggggccggga ccgcagcagg gaaggctcca tcctggctct 60gtaagacctt gaaaacacct cattcctctg gtcttggcct gctcttcggt acgccaagtt 120gctgagactg atgtggggat cagtggggag caggaatctt tctgattcag ccgtttcaaa 180gtgtcccaag cagaagctgt gatggcaatg ccaaggctat ccatggaggt ggctgtgcca 240ggggccccat ttcctgggag cccattccag gaaaggaatc ttgtagcccc aggctccagc 300agccagtgca cggcccctgg gactatccgg gtagatcaga gggaggaaca gagctgtgga 360tggtaagcag gtggcccaag tccaatttat gtctgtggtc ccagcagggt gcccaggagg 420cccctcgtaa ctcttaagaa tcttggtctg gtcagctaaa ttgtatgacc attgtactga 480gcacacatcc cgtttaagta gaattttcaa ggatgactag gagtttgcca cctgaaggca 540ggaagggcat tccaggcaga gggtacagag gtgagaggga ggctctgaca ctttgggcgt 600gcagggggtt tgatgtgact gcagctggca cacagtgtat gcccaggcct ggcacggctg 660tgttggtgtt tggagaggaa gggagaggtg agttgagccc aaggtcttcc aggccaaaag 720actgaaggtg accgcggctg tccggggctg gcccgcagac caggagggag caggtgggag 780ctggctcttg ttccggggac 8001143062DNASus scrofa 114cacaccccag gagaggaaag acccacacag tcctgatgac agcttggctc ggggctggag 60ccccgagtta taaatgtcca tcacgagctg tgttctgtca gagccatcag tgggaaggcc 120aggccagctc agcagcccaa aaatgaagag ctaggtctgg gattgggccc aagcagaggg 180cacaggaaag ccacataaac aaggcaccca acccccctgt catccaccaa tgtcacattc 240aggtcacacc cctggtcttc gggggaggtc ccctaagatc cggtggcagg gggaggaaaa 300gtctgactgg attccttgac aggtgtatca gcggaaggcc aggaggagtg ctcgggcact 360gccacctccc aggggcatga tggtcatgga ccagatggca gttatgggag gaacctcccc 420cgtggtcaga gctctgggtg ctgtacctgg tcatgcattt cgagtggaag gaaaagaaaa 480catacaactc cacccccagc agctttaggc tgttggtcta aaggtcctgc ctcctggaag 540agacacgcct ctgtcagcgg acactgctaa acctaaagga agaactgcca cctggtcacg 600ggacttccta ggccaaccaa cctacaggtg acggcccgga gcatcacgag gaggtagggg 660acgggaaggg atgcatttgc tgctcagcgg atccactggg gcgtttctgg agcccccacg 720cccacacttt actgcaaatg cacaagcccc aggcagcagg acaagtcaca gtagctctgg 780gttatccaag gagtcaggga cctacctgga agagtctaga acaggtgaca gaggagggag 840aggatggtac cagcagtata gggagaatca gaaatctgac ccaccctggg ggcctgactg 900actcccagac caaatgccac actcaggttc cccgtctgcc tgcacttcca gggctgggcc 960acgggagtta tgggccccag gtagcatcag aggctcccag gtacaggcac aagcagcaac 1020cacaggaggg atccaggcca gggagcatcc aagaagcagc agaagctcca ccttaggtac 1080agttctggca cctccaagtt gagaacatgt cctagacagt gcctgacccc aacccaatgg 1140agtgtctggg actagactag gcacgccatt ttggtcccag gttgccccat ctgtacaaag 1200ggtgtgcggc ccccaggggg acacaatgag ctcccatggg aagggtcttg cgaatctcct 1260tagaagcaga tgtaagaggt gacgtccagc ttgtgcctgg gatgtagaag tggaaaaagc 1320acccctcccc cgacaaggat gaaagcaaga ggcacaaaac aacctgaaat tcccaacgcc 1380cctggagatc cttggagaac tgggattctc cacctgtagg ggcacctgtg aggagaggct 1440gtgtgagcac ctgctgacct ggcacagagg atgcccaata ctaagaagca tcagctaaaa 1500gtctccagga attcctggaa gctgaggaag ggctcaggag agggtacaga agccctgggg 1560ctatagatat aagggacgtg cacacccact tgcaggtccc catggacccc agggacattc 1620acagtgatgg gcaagattcc caaaatgcac cccttgtgtg tgggcctggt tcggtgggtc 1680agcagacacc acaccaaagg cacaaagcac acaccctcag gctactctcc tccctctccc 1740ttgtggaaca tgagccttga gatgctgggg cacgtgaaaa acactgtcac acttaggtcc 1800tggtgaaaac tgactgcggc cagcggaaag aatcataaag accctacacc cacacacagc 1860cttaattaca gctgtgagtg gggctggagc cccaagaatg tctacaccca taagacatag 1920cgttaatcag aaaaacaaga acagccccaa ccccaccacc aggctgacaa ctaacaggtc 1980atgttggaat atcactggga atgttctagg agtgtagaaa gacacaccaa ctagggcatg 2040atgcaaagat aatacttcag cctgggagtg gatgtgacac agggaaaagc ataaagtgat 2100ggcagaggac tttgatgtca gtgatggaag ccacaaaaac ttctagctta gctccattcc 2160caacaagatt gactgcaaac cccatgctaa aacaacagca aaaagaaaga atcctcattt 2220ccaggcataa aatttttccc ccagtctctg ctgtcctcca taagatgtct gatttcaaca 2280ggaattacga ggctataaga aaggcaagaa aaaactacac actgtcaaga gaaagccatc 2340agaataacca gactcgtagc acagacactg gaattgtcag gatattttaa ataaccgtga 2400caaatacatt aaagattcta atgagaaggg ggtagacatg taagatcaca tagatttcag 2460caaagagatg aaactcgaag gaaaattaaa tgggagccct agagtgaaaa acactgtagc 2520agagaagatg ggttcatccg taaacatgac acagcttagg aaagaatcag tgaacttgaa 2580gacagggcca cagaaaatat ccaaactgaa atgcaaggag gaaaaataat gaaaggggga 2640gagagaaaaa ataaaagaac aaagcatcca agagctggag ggtgacactg aagaagagag 2700cataggcata gctggaatct cagaaagaga gaaagaaata acccaagatg taatggatga 2760gaatttcaca gaagcgttgt caagcaacaa accatacatc caagaagctc agagaacacc 2820aagcaaggta agtactgtaa aaaaatagcc cgaggtatac ctcattcagg ctgctgaaaa 2880tccatgacaa aagaagtctt gaaagtagcc agaaacagaa ggcgtgttcc attcagaggg 2940aaaagacacc attgttgcca gaaaccaaat aaaccagggc tgaaagggta aaactttttt 3000tttttttttt ttttttttgg ccatgcctgt ggcatgtgga ggtttcccga tcagggatca 3060ac 30621151300DNASus scrofa 115aaacggataa atacaggtga cccacaggca gaagctgaag tacaaacagt tcacaacggc 60acccaaaaaa taccgaaggc tcaagggtaa atctgacccc agatgaaagg ccttctcacg 120gaaaatggca aagtggcgct gagaggcatg agaggttcga atagatggag ggctccgccg 180ttttcccggg tccgaggatt cagtgacgtc acgacgccaa ttcctctgaa acgcctctct 240aggttcagtg cagcccagac ccactggcag ccgccctcgc tgcagagaca gcccagctgg 300gtcttgaggt tcctacagcg aagcaaaggg tctagaaaaa gcagacgtct ctggaaaggg 360agaagcagcc gatggattgg catacggcga caggagattc ctcggacagt ggcaccagga 420gaggggtgga cagagactgg tgcaaccgag cgggcccagg aataagtcca cacccacacg 480taccatctcg ttgtttattt attttttcct tttcagggcc actcctgggg catgtggagg 540ctccccagcc aggagtcgaa tcggagctgc agctacaagc ctaccccaca gccacagcga 600cacaggatct gagccatgtc tgcagcctac accacagctc ccggcaatat tggatcctta 660acccactgag caaggccagg gactgaaccc acgtgctcat ggatactagt tgggtttgtt 720accactgagt cacagtggga actcctttaa ttttaatttt tgaaggttca gaactcttta 780attttttagt gaggtataga ttatattacg caccatttct ttctgacttc ggtgcacggc 840ttttcaacaa atgggtgctg gacctgctgg gtgccttctt caaatgaacc acaagccctc 900cctcgcgccg tatgcaaaat ttaactcgag gggctcatag acataaacgt aaactctaaa 960gctataaaat ttccagaaga aaacgtaagg aaaacctttg gggtcttggg caaagatttc 1020ttacccatga cagcaaaatt acaatctaca gaagaactgg tggcctttat cggcatttaa 1080aacacctgcc ctttgaatga tgctgtcgca aaaccgaaca tgcagcaaaa cggatgcaac 1140tagcaggtct cacactcagt gacccacgtc agaaagggaa agacacgcca cgtgacatcc 1200cttagatgca gaatgtaaaa cacggccccc gtgaaccgac ctcaagagag agacagacct 1260acagacgcag caaatttggg gttgccgagg gggatgccgg 13001163000DNASus scrofa 116tgtgagaccc cttggcgggc caggaccccc caaggtgacc gaaggcctca gcgcccccag 60ccgccccatc cccctctttc ccgacacagg atttttttcc caccaagctc tgttcccttg 120gtcacgctct cacttgagca gcctcagggt ctcccggtgc ctgtatccac gacagcgtga 180ccttcttggt gtgtcaaccc aggaccccac gctggccagc cacgccttcc cagagcaccc 240ccgcccatcc tcagagtcca gaggaaaggc ccccattgac cccagaaacc aaaacgcaga 300gactctggga cgccagcaag aacgtacact gactcccacc tgcttcaggc acggaggcag 360gggtgggtta tgagcgaccc cgtggaaggg ccttcttgtc catcgagggg cttccagggg 420ctcctagacg gggatgagtg tggcaacatg tcgccgcatt acaaaagacc ctgcagtgct 480gctgggatgg gtcccccggc tagaaaagca aaggattcca gcccagtcga gtaggaggcg 540gcctcggagg ctgcagaggc gcggggggcg ctgaccacca ctcggcaagc cccgtgttgg 600aggggacgcc cggcccggct gcagccggtg cgcctccgga taagctccta agaggccgcg 660tgccccatgc acgcgcgtgc acacactcgc tgcccgaggg tccttcagca cagaccttgt 720ggggacggag gacctggcag gggtgtggct ctggggaagg ggtctgtccc aggaaccctg 780ttctggattt gggggtgggc gtggatatcc cgtcccaacc tacagaaggg aggggcttaa 840aaagagcccc tttggtgtga ggggccagca atcctttggc tttttcttgg cccacttgga 900gcttgacgtc tggtcagtga ctgggagcca gggccagagg ggggcagccg ggctgaggca 960ggttcaggcc aaccatctct cggccacact cccgaggtcg ggcagctacg gggcccccag 1020agacacaagc cccaggggtc cttccccccc gccccctgcc ccagatcacc aggagaccca 1080agcagctctg cctccccgtg cctgagaaat gccccatctg ggtacccaaa tcaccctccc 1140agaaggtaga gtggggggcc caggacaggg ggaccccagt tacagagccc caggcaggct 1200tcccaggggc gaggggactc cgtttggggc acagacggag gcagagcggg ctgatggatt 1260ctcccccggt tcagggatgc tggctgcctg gcctccagga gccggcggtg ccatctgatc 1320tgattaaggc ctgcagtccc agctgggcgg gcacagcctg ggggctcggc gggcagggaa 1380gaaggcgctg tcgccccagc cggtcaggct cgctttctct tcatttcctc tccattaaaa 1440gtgtcagaac catttattga ttttttaaat caggacgtgc tgtccgtgac acagcaaagt 1500gaacaaaatc agagcaaaga gaggccaggg ctgaagcccc agagggcggc gcctccaatc 1560cgggttgtgc cccggggctc caagcccctt cttcttctgg ggtcctgggc gtagtggcca 1620gggcagaatg cacctgccgt catcctggga ggcttggcca tcgctggctt ctgtctcatg 1680acgcaccgtc gttccatatc tacggaaaca gcttcgcatt aacaggcagg ggaggcggtt 1740gtttctcctt tatctgccca ccatcggcgc tggggccacg tggagcccag ccggctgact 1800tcccgctcgc acgcagggca ctgattgcag gaacgaggac atccagcccc cgcctctcaa 1860tgccccgggt gctgagagca tttcgcccaa acggcttggg tgggacaagg gatggagctg 1920tgcgccaggg gcctggctgg ggcagaaggg ggcctgcccg tgtctgcccg tggcctccag 1980caccctcggc tgccaggctg ctctggagag gtgcccgggg gccgagggcc aggggcaccc 2040tgttctgccc cacgtctctc tgtcctgctg aaagttccac cagacgcgtg ctataccctg 2100ggagtcagga ggatggggga tagttggggc ttgacgtctg tttctgaaaa aacaccgttt 2160tccctgaaat atatatgtat taatttttcg tcaagataaa actgtgtata gtttttcgtg 2220atgagaaaac gcatccatct tccttagaaa gcctgaagag gtacaggagc ctataaagga 2280caagatgaca gatgcctcta acgcacacca aatgtgcggt ggcccccagg ggaccgcata 2340gacggggcgg ctccagatgg ccaccgtgtg cgagggacac ggttcagggt ggcagagtat 2400tcctgggggg ggggggctca gcggttccca tttccccctc ccttccttcc ttcatttctt 2460tccttctttc tttctttttg tggttttagg gccgcacccg cggcgtgtgg aggttcccag 2520cctaggggtc taatcagagc tacagctgcc ggcctccacc acagctcacg gcaacgccgg 2580atccttaacc cacggagcga gaccagggat ggaacctggg acctcatgga tcttagttgg 2640gtttgttccc gctgagccac aacgggaact ccagccattc ccatttcttg ctccagttcc 2700aagaattcca attcttattc ctgttcttta aggccagagg cgacagccac gccgagtccc 2760agaagcaggg ctcaaggatg ctgctgttga ctgtgtccgt gggcgggggg agttgataag 2820aacccccaac acagggtggt ggccagcaac gggggaggga ggaggggggc tggtggggaa 2880aagtcccctg aaccccatgg gctgccccct ccaggctggg gcacgacccc gagccccatg 2940gcccgaggag aaacggtccc agccccaggc tgggctcccg cacccctgcc ctgaccccgc 30001171895DNASus scrofa 117tcatggaagc ccttatcaca acctcggatc caaaacccac tgcgcgagtc cagggataga 60actcgcatcc ccacagaccc tatgttgggg tcttaaccag ctgagccaca tggaaactgg 120gtaatctatt tttagatgtt cctagggttt ttggccttgc ctgtacgtgg ggacgctgct 180gggccaggga tcaaacccgc gccacagctg tgacccaagc agagcagtga cagcaccgga 240tccttaagca cgaggccagc agggagcccc tgtgtttaga ttttggtgag gatactgcgt 300gggattcagg atattcactt tggggctgtt ggaattgccc gtcgctgttt aagcaaagag 360aaatcccttc actctgtgta actgtgggga aatcctttag tctcttgaaa ccattgcgtg 420tgtttaagag tggtaactct gccaccataa atgcccagac cagcgccttc ctgagatccg 480cttttgttgc aaatatctgg tttgaatgct ttgatcgccc gcaccagacc agggtgggcg 540gacgccgccg gggacccgac gtgaccatcg tgcttctgta tccgcccttt ctccggcacg 600cgccccctgg ttgcctctgg ctgcttttag tggaggaact gaagcctcgc cacccagacc 660ccgagaccgc aggacccaca atgcttcaaa cacctgccct ctgactttta caggtcaagt 720tcgccaacgc cgaatttgca ccgattggct acagagagca cggtggcgcc aagcctccac 780ttggagtttt ataaggtctc cctccagctc gcaatgaaaa tgagctgtga taaggcaaag 840acaaaattag tatgaaatcc agatgcttca tctacaatac aatgaccgcg ggatttgggt 900ctgagcgact gaaatcaagg tgggcttccg gagggaggct gttagaggaa aggcattcac 960ggaggctcag gtccgagagg cttccacacc cctaagaggg ctgagacggc aagtagggac 1020caagccccgc agtcgggaga gctgggcagg aaggaagtct gaggtcaccc ccacctgggg 1080aggaactgcc tagagaagcg ggggcgggaa gcaggggatg cccagtccca agacagggac 1140agggcggaaa gggctctctg caggccctca atgctgccac agtgtcctcg taagagggag 1200gcagagagaa ttgacaccgg ggagaccacg ggaccacgga ggtggagacc gggctgcccg 1260cgcgtgccag ttgctcccga agccggcccc tcccccagag cctttgggaa gaggcgccaa 1320cctgcagttc tgctactcgg ggacagggac agggacagcc ccctggagcc gcctcttagg 1380ggcagcatcc cccagaacct tccttaacag accatctgga gagagatggg tctgggctgc 1440agctcctgga actgttttgc ccacccggcg agcaccagtg ggtgccagcc tgggctgccc 1500agcctcaggg ccggggaggg ctgagggcac tggggcccgg ctctgggact cccctgcctc 1560ctgcccgtgc aggacagcca cctcccagca tctgcttcct gccacccaca tccccaggac 1620cgtcagccca ggcatgcccc tggcgtcggc cactcacacc acaggccagg aacccaaggg 1680ggcaacacag aagggcagtt gccatctgca gatggaatgg acaaactggg gtccgtgatg 1740atggcaggct ctgggcgccc gggctggcag gggagccagg actgtgcggc catcacagga 1800agggcatgac ggggtgaaag caagagtgga aacctctgcc acccgcctgg gcgcacatac 1860cggccaccct gcagccccac ccccatttgt ttgct 18951181040DNASus scrofa 118gcgcgccgga tccttaatta agtctgagag atctgcggcc gcggccaggg tctgcttctg 60gccaagtgtg gggctctgct ccatcctggc tcggaggtcc acccatggca aagcctgggg 120tcctcccact gaatatttgg gggtccactc gtgccaaagg ctgggtgtcc agtgtgccaa 180cggtacatgg aagcaatgtc ttcccaagga ccgtccaagg tgtggtcagg cctggacagc 240tgtgagtccc ttcgggacta gacttggtgg ccgaacccta gggaccgtgc ccgagggccc 300ccacgaggcc aggtgtttgc cccagggaca gaacggccaa gggtggccga gggttctttt 360tgtttgtttt ttcttctttc tctttttctt tggccgaggg ttcttaaagc gctctctctg 420ctctttgtcc cgatcctgag cgggcagtgt cctggtcggt ggggtgctgg gcagccgcag 480cagggctgag agagcccggc ttgtcactag ggcgcgccgg tgagcccagc gggcatgccg 540tgtccagacg ttggatgggg cagcgagggg actggggtgc cccagccccc gtgggaagcc 600cgccctgtgg aagccgctgt gctcgccaca acaagcaccg tcgactagct ggtgaatcag 660cgcccgtcgc ccgcgtaatc ccaggcgctt tctgcccaac ctgagccctg accccacacc 720ccttgcgacc gctccgtgga ccctggggcg atgaggtgaa ccgtgggctt ggccatcgtg 780gtggcagacg gtggcacacc cgtgcgcctg tcggcccccc tccatccagg agcagagtgc 840gcacccagtg ggggctgggc agggagccgc ctccacctcc gccctgaggg gacgggactc 900tttcgacccg gagtgggaag ggacatatgc ggacgatgcc agaccctgtc tgtgggggga 960gggggagaag gccctctttg gagaattcca ggacgggtga ggaacgtgtg ctggaccggc 1020cgggtcggag gtgggccttg 10401199236DNASus scrofa 119ggcaaccagg ggaagatggg gaagcggggt gcaggggcgt ttgcgcgggc caaggaccac 60cttggaaatc tggagcctgg caggagcggc gcagggttga ggggctggct tgggcagggc 120tggctggcac ctgggagcct ggcggggttg aggtccgggc tcccaggtgc cctataggca 180gggcaacatc ggcatggggg gtgacaggcc cgagctgggg tgcggaggga agagggggga 240gccaggcatt catcccggtc aattttggtt tcaggtcgtg gcggctggtg gtcaggggga 300gttggagaga ggttcgcccc ggggcctggg gcagcggagg tgtagctggc agctgtgggc 360aggtgaggac agccgtctgc cgggccaggt gagtcccctt ccctccccag gccttgtttc 420tctggcctcc tgcatccgga ggttctgggg agcgagggcc ggcgaggcga agcggctgac 480cccccggcag agtggcggcg gacgacaggc aaggcgggca gaacaggtga cacgtctcag 540ggggagctgg gaccgggcgg ggctgggggg ccggggccgt cccaggtgga aagagcatct 600caagcgagtc tggtgggaga cgaggcaggg ctgccagcag ggaggagacg caacaggcgg 660ggggcattcc aggcccgggt cggacaggac ccgtcggggg tgtcaggaca gtggggtccc 720cagccgccac ttcacccact gcaattcatt tagtagcagg tacaggagcg gctctggccg 780ggcctcttga ggcctgagct ggagcctcga gggccggaga atgggaaaga aggtgcagtg 840tgccagacag acgtcacctg gagggagcac ggccgtgggg acgggcccca gagagatttc 900ggcagcaggg aggctgcgcg ggcccagcct gcggacgtgc gttcccacgc agcactgcgg 960cccaggggct ggcgcggcag ggcccccggt gtccttggtg gcactgtgcg ccctcgccgc 1020tcgcccctgg gactggcacg gcagacagga cagcacccag gggagtcaag ggcactgacg 1080agaccagact aggcgaggcg ggtggggtgg aatggatgtg acctctgggg ggagggaggt 1140ggggacgcag gcaggggcga ggcgccggag cctggcggcg agcgaggcca aggcgggcct 1200ctgcgggtga caactgagca catatgggta cctttgcgct cgcaccggag acaggtgagt 1260gtctggcccc ggcctgccgc cctcccggcc ccgccactgc ctctgccctc cccctcgacc 1320agggccctct gcttccccac agcctcgtct ccagtggggg tggacacact gccagcacca 1380caggccggac gccaggatgt gcttggaggg acatgacaca gtccggtgtg acggagaggg 1440acagacgtga cgccgtccgg ccttcctggt gagcgcaggt ccaggccttg gcccccaggc 1500cagccgcccc accccccacc cctcatggcc gtcttctgtc ccgcagaaca ctctcggctg 1560gccccgcggg ggagctgcca cacccagcgt ctgttccttt gccttcctga aggagcacgt 1620gcatgactgc tgctctctgg accccagaac cctcaaacga caaggtgagg caggtcccgc 1680ctcgccccac acgtggaagg ggcgtgggcg agagccgggc gctcacggtg cccccctccc 1740cctgcagaga tggtgctacc cagctcatgc ctgggccttg gacccggact tcttcaagtc 1800ctcctagctc tgactcaaga atatgctgca ttctggagcc actacactac ttgactcagg 1860aatcagctct ggaaggtggg cgcgcgctcc tcccgctccc ggagccccgc ccgctgcccg 1920ctccccgctc acgtcctgtc tctgtcctcg tccgcaggtt gagccaaagg aacagacgtc 1980ccacaccacc ggaccaacgg cacccgcggg gttccccacc ccccgcccgg ccactccacc 2040tcggcggcca ccccctgctg cgccctggag acaccaccag cctccctctc tccccttcct 2100cctttttttc ctctgtcttt tctcttctct tctttcctct cctttgctca gaagactcgg 2160ggcatccagg actctgtgtc cccgtccttc ctgaattaat ttgcactaag tcgtttgcac 2220tggtttggag tcctggaacc agccccgggt ctcggagcgg gtgtgtgagc tgccgagtgg 2280cctggcctcc tcggcccgcg ccccctcagc acctgccatt gtccatctct gtctgggggt 2340gactgggtgg gggcctgagt gtgtggggcc ccgccctccc ctctcctagt ctggaagctc 2400cgaccaccga gcagacctca aacgctgcac tgagtgtcca tctcgtcatg tgcccctcct 2460cgccagggcc accccagagc cctggactca tcaataaact cagttaccgg aatctgtctc 2520aggggctttg caattgggct gggggtgcgc cggggaaggg ggggatgaga tggggaacat 2580gcaaggaagg gcctgtgggc tgggggacac agaatgggtg gggagggggc tcacaggact 2640cggggggtaa tgaacgtggg gctgggcgca aaggggagtg ggacgtgggg atcagggcgg 2700ggggcctgga ggatgcaggg tccctgcagg gaaagggggc cgagggcgtg aggcatgtcc 2760tcagccctga gaggccctac cccacaaagc acagcctgcg cgcgacctcc aggcccccaa 2820acccccgccc cagaccctga agccctggtc cagggcagtg ggtctgactg gcggaaggaa 2880catgccaccc aggctggcca caccactggg acgcccatgg gcggccactt tcatcaagag 2940cctggcaggc cctgagtgct gggctggagg gcacagaggg tccccctccc ctcacgcttt 3000gcggtgctgg ggcaccgcag gagtgcccaa caggagaccc caggaagtct gctgggctgc 3060agcgaagggc agggtagggg ggcggcccac aggggcccag ctcagtaggc aggtggcagt 3120gggaggcggc agaaagttgg aaagggtgga ctgggcacgt caggatctcg tggcggcagc 3180cccggagcca cggccttggg tgcactgcag cccccacggt tggtgtcccg gtcccaggca 3240gcagctgggc tggtgacgcc cctctgcctc tgcccacccc ccccaccgcc cccccgccag 3300cctcccagcc cctgggcgcc tggcgtgacg ctgggaacgc gagggagcag gcctcggaaa 3360cagggctggg tccttgaccc cttcctctgc tcagggcagt caggaaatgc ctagcgggcc 3420gactgaccga gaggagatag cggaggcctg ggagaccccg cgctcgtgcc gttcccagcg

3480tccggccgcg tggcccttgg ctggcctggt ttgggcccca tgagctcacc ccccgccccc 3540cacagcctcc ccgcgtctgg tctcctctct gggccctgct gtccctcctg acgggggaca 3600gagccctcca gggccccggg gggacggtcc cgggtcagca gggcgggtgg gcagcacagc 3660tgcgtttggt gaagcccctg cccaaagcac cctcagcgtt tcctctgcgc gtccggccgc 3720ccccggaggc tttcccaagt ccacgggcaa ctcgcaggcg agcccactcc acctccatca 3780cgcgggtttg gccagcggca gaagcactcg cccttcaggc gtcaggagtt aagcccctcc 3840aaggcccggt gctaatcagc tgcctctcct ggagcttcgc aaagcgggct ctcagagccc 3900agcttcccgg gggctcaccg tggtggcatg ggcaccacag gtggccggag gggcaccgag 3960cacgacgggg ctgtgggggg tggaggaggg aggttggtga ctccgaacct ctactgaggc 4020acacagagga cacggccgct tccaggggag tcagcctgcg aagggcagag gggctgtagc 4080ctcccggtca cgccctcgcc tctgccctgg attcctcctg ggggcccgcg gctcgtcggg 4140gaggtgagtg cccctggatg ggcgtaggct gggggggcag ggagtggggg agccccgagg 4200ccctgggccc acagccctgt cttgccccac acacagggct gtctacactg ggtgcccact 4260tgctctgctt ctaggctgtt ccctgggcag ctgcctggag ggccgtgggc acagtgcggg 4320cagccagtgg ggaggccggg gatggggccg gggataggga cccctgcccc tgggtgagcc 4380ccacctgggc tgggaagaca gcagcagcgc cccttcaggt ccatggacca ggggacccag 4440ggtggactgt gtttaccttc agcccaggcc agtttcctgc ttgagaaagc ccgggagggg 4500gtgcgggaca ggcccgggcc ccccacgcaa aggcagtttc gcaatgtccc tgcgctgact 4560gaaatgtcac caggcacacg gcttgaattt ctcccccaga cctggcaggg gcgggggtgg 4620gggcaccggg ctgctgggat cttggcccct gaacctcccc cggccctgcg gccagggagg 4680gtttaggctg agtgacagcc cacggaaacc tggacccgac atgtctgtgt gtccatgtgt 4740gtctgtgtgt gcgtccacct atgcgtctgc gtgtgtgtcc atgtgtgtcc acatatctgt 4800gtccacgtgt ctgtgtccac gtgtctgtgt ccacgtgtgt gtccacgtgt gtccatgtgt 4860ctatgagtcc ttgtgtgcat ctgtgtgccc gtgtgtctgt gtgtctgtcc cctgcagtcc 4920ccgtggacct gtctcttata cacatctcaa cctggcagcg ccccttcagg tccatggacc 4980aggggaccca gggtggactg tgtttacctt cagcccaggc cagtttcctg cttgagaaag 5040cccgggaggg ggtgcgggac aggcccgggc cccccacgca aaggcagttt cgcaatgtcc 5100ctgcgctgac tgaaatgtca ccaggcacac ggcttgaatt tctcccccag acctggcagg 5160ggcgggggtg ggggcaccgg gctgctggga tcttggcccc tgaacctccc ccggccctgc 5220ggccagggag ggtttaggct gagtgacagc ccacggaaac ctggacccga catgtctgtg 5280tgtccatgtg tgtctgtgtg tgcgtccacc tatgcgtctg cgtgtgtgtc catgtgtgtc 5340cacatatctg tgtccacgtg tctgtgtcca cgtgtctgtg tccacgtgtg tgtccacgtg 5400tgtccatgtg tctatgagtc cttgtgtgca tctgtgtgcc cgtgtgtctg tgtgtctgtc 5460ccctgcagtc cccgtggacc tgtgtggtct ctggtgtgca gccctagccg cggcccgtcc 5520caggctgagt gtccccaggg tgcagcacag ctgtgacgag ggtgtgggtc ccgctggccg 5580tgtcgctggg ctgtgggccc tatcctcttt gtggctgctc tgcaaggcct gatggctttt 5640gtgtggcctg gccgttcggg tccatgcccc ctggaagagc aacgtctgag ctagctccac 5700gcgtgggtcc atctcggccc aggtttaatg agccactttc aggcagggat tgcacaggag 5760gcagggtggg aagtggctct gctcagaccc ctgaacaggg tctggagatt ctccaagggc 5820acaaaagaac ggacgatgcc cctggggtca gcgacaatgc tccctgagaa atcttggcac 5880acagggctgg gcctgcgagg tggcccctcg ccccacccca gcctcctgga ggacaaccgt 5940cgccctgctc ccagagctgg ggggcgccac acgtggggca cagggagcat gggcccgatt 6000ccaggcctgg gctccctctc gtgtccagga tctccccgtg tcttgtctca acaagcccct 6060gacttggagg ccccagggtg accccttaaa gggggaacag aaggttctag aaggagcgtg 6120gccagctttg gcttccctag ggctgtggtg accacactgg gccacggccc aggccacccc 6180acccgcctcc ttccccctgg ccccctccct tccccgcacc tctccctggc ctgcacctgg 6240tgacacggct ggctcccagc cagggctgag ggggaccagc ggggcccctt cctggaagcc 6300cacctgcagg ccggcttgct gggaaggggc ctgctcctcg ccggccccac ccgcccgggg 6360ccgtttcctg gaagcggtca ctggatattt tgttccttgt cagcgccgag cttgcataaa 6420gcagacactg agctccttgt cctccgggag cacgcgctcc atcaccgaac acctggccgg 6480acacaggcgg gcagccgggc ctgggggagc agcgcgggcc tggggccgga ccagcaaacg 6540atcacggcgc cgagcgcagg gcccgcgccg cttctgcagg ccgcccccac gtgcccaggc 6600ccagcggtgc ccatcctgca ggctgggagg aggctgtggg cgcagagctg agaagggggc 6660agaggcactg gggggggaca gccgtgttcc cacactttgc agaaaccttg gccggcctgg 6720atgtcttgct gggagagctg ggggagggga cagggcagga agccggtccc cccgagcggg 6780gtaggaagag gcctcggccc tgggaggagg aggaggggag ggcagtgaga tggaaagagc 6840accaggggct cgaggcttct ttctggaaca aggactagaa ggaggaggcc gggcagctgc 6900ttgggatgct tggaacaggc cggccccagt gctgacaggg acgtgacctg ggggccggtc 6960ccgggcccag gcgggctggg agggcgcctg gtgggtcagc gccactcaga gccctggcag 7020cagggggcct gggcacggct gcaggacaga gctcaggaca cagatggggg cgaggactga 7080gtggggcacc acagatgctc ccaggaggtg gccaaggagt ggccttggga tcccaggatg 7140gccctggtcc cagaagatgc ggcagcccaa gggaccaggc cagggccgca gggggccaca 7200atctgagcag ggctcaggcc cagggcagag gccccctccc acccagccct ccctgggccc 7260gcctctccgt gcaggcagtg ggctcagatg gggcagacat gagaccaggt ccagggagaa 7320gcggggcccc ttggcttcat tcaggtggct ttcagaccgc gccccgtgcg tggcaaggcc 7380cacagcgctc aggagcacac agacccccac cacgggctcc cccaggttgg gcggtgacat 7440cagccctgtg tcaacagcag gagctggcag ctccccaccg gggcttaggg agcggggacc 7500ctgagccacc ctgccaccgc cccaccccac cgtggcccac acgagggccc gctgctctgg 7560gtctggggcc aaggcccccc aggcgcctgg cactgtctgc ccctcccgct ggctctccgt 7620ctccagtgtc cccgccagag agcatggggc cacaggcctg aatgccaccc tcttcctccc 7680tctggagggg gcctgaggtt ttgggggttc acagagtggc ctccggggtg ggtccaggcc 7740cagcgaggca aagcggaccc cagggagtcc cgcggaatgt gggacagccc ccccgtagat 7800ctcggggggg ccaagctctg gttgacctcc atcctggggc tgtgggcctt tggtcagtgg 7860ggagggtcat gacacccagc ccaccagctg gtgacagccc tggacgtgcc ggctcagggc 7920tggcctgccc ctgcagcctt gaacccctgt tctctgggag tgggggcgca gggggcgccg 7980gggcagggtg agagacgaga gcctctcttc ccagaacttc tgcctgcgat gaggacccag 8040caggggcctc tcctcaccag agggcctctg ccggctgcag ggccccagag aggcccagag 8100gctggaggcc gggccttggg aagaggccgg acttccagaa accagctgcc cgctccgcag 8160cacccagcgc ccacttggga ggggggcgcg cccccgtgcc ccgcccgggt ccactgctgg 8220ggccgccaca ataaagtttg tccctgctgg ttactgtccg tgtctgagag gtttctggag 8280cctggccaca atgggcgtca ggatgcggct gggagggagc ctcgcgagtc agagtgtgct 8340ggtctcggac aggccccggc gcccccagcc cgtgctctgt ggacagatgg gtgggtgggt 8400gggtgtcgga gggggttgga gagggtgggc gggacgaggg gcttcctgca ctctgtccca 8460gggaagcggg gaccaaggag gggacagccc ccggtcacca ggagggtcct gtccctctca 8520ccccccggga caggtgagct ccccggagcc gcccttctgg gacaggaccc cacggccagg 8580ccacggcccc ccccaccccg tggtccctcc gtcccacggc cggcctgggg ggccacgggc 8640ccagggcccc cgctccccgt tggccctccg agggtgaacg acctcgcctg ggacgtgggg 8700cagagggcag gcgccaagag tgaccccctg ggacacgtgg ctgtttgcag ttctggaggc 8760agccgagata aagcggctgt tttcccagtg ggctcagggc cagagggggg cgaggggcag 8820ccccagtcaa ggccgggccg ctgcctcggg ctcccctctg tgcggaggga gggggccggt 8880tgcacagcag cccctgcccg ccgcccgccc gccggcgcag gcaccgtggg acccggcctg 8940gtgcccctcc cccgcccctg ctcaggggcc agccctctct ggttcccagg acgcccccgc 9000cccgcaggcg gccagagagt cccagagtgt tagcctccca cgtgtgggat cctgtcatat 9060gcgacagctt aactcaggcc gaatttcatg ggtcctggat ttgggtgggc acggcccctg 9120cacagcgggg ctggaagcct aaggcggtgg gcgtgggggt gagaggcccg cagacaacag 9180gagggaggct gggacacttc aagggttgac atgctatgcc tgtcacggat aaatgc 92361205349DNASus scrofa 120agatgtgtat aagagacagg ggctgggtgg gaaggacaga gggtggggcc ggaggaaatg 60ggatgcagag cccaccgtgc acgctctgct ggcctttgag cctcgctgag tcgcaagaag 120ccctcgggcc tggaaacaga cccccggccc ccacccccac cccggccccc ggattacccc 180ggcatggctg gagggcccga gaagccaccc aggcttcccg tgccgagctg ggtgctgggc 240ccagccgagc gggcttgacg ccacgcttag ccctccccag ggagcccagg gtcggaagga 300agaggccggc cggagggccg tggccgctca ggctggaggg ggcccccggg tcaggatggg 360ccccagacgt ccccgctccc cggccatccg tcacggagct gtcacccagg aacgtgctcc 420agacgtgctt tcctgccgcc gaggccccga gcaggctcca ggcgccccca cccccgaacg 480cccacgcaca ccctcggtct gcgaacaccc tgccgtcatc cggtggcccc ggttcccgcc 540gcccgcgcca tccgggtgcc ccttcctccc tgggtcgggg gccatgccct cagcgggcac 600gcaggcctgt gcaggtctgt tctgactctt ccccaaagac gcaggccggc tgcgggcgcc 660ccgacctcgt ctgaggcccg tttgtgctca ctggctgtct cagaaagggg tgcccacggg 720aagcgcgtgt tccttgggcc gcaaggcaag ggagcccacc ccaaggtggc tgagggcaaa 780tggcccaggg cctctaagga gtccctgggg gccgggccgg cctgcagctt gaggaggaga 840gccctggctc tgctcccccg ggcaggtgag cccacggcag ggggctcccc agcagccttg 900gcaggaagca gtgaggaagg ggtgaggatg aaggcaaggg ggcctgcggg gacttgggca 960aagcccctga agaactgagt tcctcggaaa ggccggagcc ctcagccgag cctcggcctc 1020cgagcgatgg aggcggccca cctgcggccc cagggtgcag ctgtgcatcc gtccccctcg 1080ggcctccccc tgcccccccg gccaccacac tctccccctt ttgcctttga tcacttgagt 1140gcgacagctt gtgcggcctg agccccagag accgctgccc ccctgccgcc agccccacgg 1200gagcgtccac ctgggcctgg cctgggcact catccctccc ggatgaggcc tttctagcct 1260gggccgcccc gggagcggca gacccagccc ctcgcccccc tcccccagtg aaggtgctgc 1320ctggtggtct ggggaagccc ctggaacagg gggcgcaggt cccacacggg tgctctggcc 1380tccagctgcc agggagggcc gcgctcaggc cagggtcccc tccaccagaa ccgccagggc 1440cctggggaaa acctgtctgt gctaacaggg ccgctccccg ggactccacg gagaggtgcg 1500agggacccct gagcacccac cgccactaag gggcccagcc agctcgcggg tgcaggcagc 1560cggctgggcg ctcacatgca tactgctctc tggctttgtg tgtgcgcctg ggttggggtg 1620agcggaggtg cccgaaggcg gaagagccca ccctccactc ggggacctat ttcagcaaga 1680agacggatgg gactgccggg catggacaaa ggaacaggat gaaccttctg gaacgcacaa 1740ggcttccacg gctgaccggt cataggaagg cgcgtctcta ggccaatcca ccgtccaccg 1800tccattcccc agccctcgag agggggcagg atggaccgct gcagcgtgag agagctctgg 1860ggcgctccca cagggcaaag tcccagggca ctgacctcag agcccaacca ggccaccggg 1920gctgggccca ccagggagcc ggggccaggg tcagggtcag ggcccagagt gcgggaaagg 1980gtggcgtgtt gcttggggcg gcgggcgcgc agacggcccc tcgcaccccc cgacagccct 2040ggagctgagt gaagcccgcg ggtcaccttg gctggggttg gggtctcctg cgaccggcac 2100cccagctcag gtcatccttg ctgtaccgca gaggggcagg ggttctgagc agggacaggg 2160tgggccgcgc aggaagcccc cttctctctg aggctgcccc ggccctggag cctctctggg 2220gcatgccacc cctctcacag acgcctccca ggagccccca ctttcctgct gcgtggtgag 2280ggtgtctctc acccgattcc tggcccctgc aggtcgagtg agtccctgct aagcctgggg 2340ttggagcagg tgcagggcat caccacacag cagcagaggc tgtgggggcc cctgagaggc 2400gctcccaggt accctcctca gggggctgag cccggggttg acccgggacc tcgcctgccc 2460caaagccggc gccctcctcc cgcccgcccg accagggcca gagaagcagg tgtggggcgg 2520cacaaaccca agtcagcttc cagatcctgc tggggccgcg ttgaaactcg aagcccccag 2580gctgggaggt ctagacaccc ctgcccagac cgacagcctg ggcctggctc acagctgcct 2640gggggcccag gggtgcacct gccctgtggg tgggggtcag agggcaggga accctcggga 2700aggtccccca gggtcaaggt tgggcctaag ctccggtgac ctctgggaag tctggggctg 2760ggttttgttc ccagaggaga gagggccagt agcctcagag gggctgtggc acggtgggaa 2820ggccccaggt gaccccagag cgtgcgaagc aagccccctt gactgcaaag cgcaaagggc 2880agaggtgggg tgggagcctc gaccccccga gcccaggtac acagggggaa gggcgaggga 2940tccggcaggg gccacacccg ccaccccagg cagcccacaa agcctttggg cccggagccc 3000cagatgggcc cagcccagct ctgggaacag tcttcccaga attccccagc tctgggtacc 3060aacagggctg cccggccccc agagccctcg ggcgggagac ccttccccag ggggatctcc 3120taagtggcaa ggcctgttgg gaggggctgg tgagaggcca ctctggcggg aagaccccca 3180gccacctgga gcccctagcc actgcctgct gcggctccct agggatccag ggccatcaga 3240gaagctccag cgacactgtt tattttcaaa tgacactttt taagaaaaac agcctcaccc 3300aaatgcttgg ccctgagtct ggaatgtgca gacagacagc tgcccctccc cagagcctgc 3360acggccctcc gggtggggga ggagcagggg gcacccctgg gaccgggccg caggctgtca 3420gggcacggaa cgtgtctctg ggccctgtcc tcaattcccg gtgcccagtg gccccaactt 3480cccagcagac ccagcagggc cccagcttgt cttggcctgg ccgctggtcc tgtcacccca 3540ggcctggagt tctggaagat tctgctcctg ctcccgtgtg cacataccac tccccggggc 3600agccctgcac ttctgttcct gctgggctcc ctgcctgcat ccgtgaggcc tgcagcccgc 3660ctgatcttcc aggtcctcct ccgagccccc gcctccagga agccctccag gagagctcag 3720gagggtcggc tccctgcgcg cagctgtcag acccctgggc ccaccccgcc ggctgctagg 3780gtccaggttc cccacaagcc ctcgggcaga ggctgggccg ctgggtccct cggagacaac 3840tggctccgag gccttgccct agacgggttt ccgggagccc gtccccagcg gcacccactg 3900agttttgaac acttggcgcc acccccacac cccaggcggt ggccaggagg cctcctgggc 3960agcagacagt ccgtgaggtg gccctggggt ggctcctgac ctgggcgctg gcccagccct 4020gggcacagct ttccagatct tgcctgccgc ttcctccagg ctgcctcggc ccctcccgcc 4080tgggggtgcc cagcttttcc tggaggatgc ccacccttgc ccatggtcag ggaggggctg 4140agaaacccca cctcgtgcct ctgcccggcc tatgccaggg gaaccaggtt ccctcccgca 4200ggaggggacc gagtccctga cagcccactg cagaggggag gaggtgcctg gctctgcccc 4260cagccccacc aaccccgtgg cttcctgttt cgcagcccac aaagcactaa aggccgcagg 4320tcctggaaca tcaaagaccc gggaagtcca ttgtattgaa ttgagtgtaa atgagcctga 4380ggcctgtggc ttgcgtttcc cacaattacc gctgcccggg aagggctccg gaaccgacac 4440agcccccagg gccccttgcc catgtgggga gcccaggctg gcctgaagaa gccccataag 4500gtggacccca ctttgagccc ccacgagagt gggccaagga ccaggtcagg ggctgcccag 4560gctctgggcc tcctctgcct gccaggtggg ctccctcggg gcccagcctg gcctgcagga 4620ccttcccacg ctgagttccc cagcctggta tgagcgtagt ggacggcagc catgcccagc 4680actcaggggc ctgagggaca gagcgggaac tccagccccc gggtcctcgg cccctaggat 4740ccttctaggt ggggaagccc aagggagcag aggggtgaac gcagctgtgt ggggccccag 4800gctgccgagc agacccctcc tgctccactc ctcggccgag tgggcgccga gatgccgggg 4860cagtgccatt tcccaggcgc caccggaggc tcccagaggg agtgaggcac gagctgggag 4920ggagggcggg ggggctgggg aggcagagag cggaggccgg aggccggtga ggaggcccgg 4980agggggcctg gagtcaatga cccagggatt atcgtgctgg gtctttgcaa agttggctga 5040gcaaacgccg gagccaaggg tcagggagac gggactggcg gggccccgcg gccccctttc 5100ccctttctgg aaaaagcctg tttcccaggt caaaatccag ctcatgatcc gccccctttg 5160ggactgatgt tcagaggccc agtggtccca gcacctctgt ccaccgcccc ccccacgctc 5220ccggggccgc caacccctgt gggctgcgag gtgcgggcac ctctcccttc gaagcaaagc 5280cctgccctgc gtgggcagcg tgatttcctg cttctctggg gctgcacttt gactggggtg 5340ggggggtgg 53491211593DNASus scrofamisc_feature(1551)..(1551)n is a, c, g, or t 121agcccctcag cccctccgag cagctgctgg gctcagcggg ctcgcccccc gatgtgcggc 60cctccataat caatcatgga gggccgggcc cggggggggc gggccgacct gtcagccagc 120tccaagggca gggacagctg ctgttccgga gggttcccag gggccagccc caccagacag 180cggcctcggc cccccttccc cgaggggcac ccccacggag ggcccagacc ggagggactc 240ggggcccaga ggccagggca agagtgaagg cagcgccggt gggagcggcg gtcagcgggg 300tccaggcttc agttcccaag gagccccatg ccctgagccc gcactgagcc ctgtgcagcc 360tgtgggtgcc gccgaggccc gccaccccgc cccccaccag cctggggtcg aaggagggag 420ggggtggcct gacggatggt aacagctgct ccccccacct cgccggcgtg gacagggctc 480gcttctcctg cccgagcccc cggctgcccc atccgtcacg gcccacccag gactgtgcgt 540ccagcctccc tccctcctaa tccccccgca ttttccgaat tctcgggcca ctgctgcttc 600ctcctcaaat tcctggcccc cctcgcccca tccccgccat gggaaagggc cgcgatgcca 660ggacacttgc tcgtctcggc cgggcggggg gaggagcagc tggctgggcc cggcagctgt 720gaggtgcggg ggtgccaggg agaagggccc agattagggg gcgtcatggg aaagctggga 780gggaacgcta cccagagccc ctcctgccgc agcctgtgct gctccctctc cgcatttctg 840gcctctgagt gctccctgga gggaagggac cactgtgtcc tgccggcctc tggctctgcc 900aggaatgtcc atctgtccgg gccgggttac ctggctcaga gcgtgggtac cagctcatcc 960agccctgacg cctgctctcg ggaacagtgg atgggccagg cgcccccgtc acaccccgca 1020gctgggctcc acagacgggc ccgggatggc cacggaggtg gggggcggcc ccagggcgag 1080gctccctcct ggaagggcta gagtgtgggc tgcgcggaga gggaggccgg acggccaggc 1140caggtgcagc ccggggcagg tgctggtggg ggctgtgacc cacgtgtgca gctcaagggt 1200ccaggagccc cagggacaga gcctcaggga cagaccctca gagccacagc aggaagcctg 1260gtggcagtag ctggcggggc cgtggggtgc tcggccctgc agacagaggc agaggcaggc 1320tccctgctga tgacaggggc tttctctgtc ccctgggggg cggagggggc ccgaccatgg 1380accccgggcc tcctctcgca cgattcccag gccagcctgg tctcaggcag tccaaggttg 1440cacaatggtc tccatcgtcc agagttgcag agccagcact ctcccactgg acggcggccc 1500ggggtgggct gcaccgccgc tcagggctca gggccgcggc cggccagccc nccgcaggcc 1560ttgaccctgt ctcttataca catctcaacc ctg 1593122831DNASus scrofa 122tgagatgtgt ataagagaca ggccttgacc ctgggcctgg ctcagctgcg cgccctcctc 60cttgcagctc cgcctcgacc ccatccatca gccattttcc tacccttcct gtaataaaaa 120acccgaagcg gcgtggcccc gtgtccgctg gggtgactgc ggcctgcctg ctggtggctc 180ccacctgggc ccggccccct gaaaacacac acccggcgat ggcttgcccg gggccctggt 240ggaggggcgg ggggcctcgc ctgcctcttg tctgaaattt tcggtcccac atgccccgac 300tcctctcccg gcccaccctg caggcccggc cggtgccccg gccactttcc cgaaggacgg 360actcagcatt tcccagggca cctgctgatg gtgcccagac cccgggggcc ttcccgccgg 420gcgcggcccc acgtcgcccc tccagtggcc acagcgggcc tgggccaagg ctgggagttc 480tgcacgggcc tgggggagga aggcggggga gaggggacag tctcctggcg gggacgaggg 540tgggggcagc aggtggggag ttcccacagc cggggcagcg ggacgccgct tggctgccct 600gggtctcagc cggggacagt gcccaccagg agagagacgg cagacagtac agcccacccg 660ttttatatcc tctcaggcgg tctgtgcttt attggggtaa atatgcagga catagaaact 720ctgccactgg accccttggc cgggggacac agcagcggca ttgcatgctt tctgggtgca 780gcgcagccag caccaccggc cagagcaccc catcttcccg atcaaccgga c 8311234636DNASus scrofa 123ctctgggcta gcaccgtggg ggctttgcca gagtggaact gaactgggtc caccccggag 60cccagagggc ggtgaatggg aggcagagcc catcctggga atggaccaga agaaagggag 120cgggggtggg ggaaggggca tcagatcctg gtccttcctt gtcgcctgcg gtccctctgc 180caccactccc cgaagctgat ctggagcaca cgcgtcgtta aagccgccat cgaggcccca 240cttctgacag acggaagggg gcagagtgcc ttcctcaccg gcctcgccct gggaaggccc 300ctccctgcag cccaggaagc cagcagcagg tgacagagcc aggggcccag ggccccaggg 360acgggctcgc gcgcccgagc cgggggtccc ttggcgtccc catcctctcg tcctggagcc 420ctcctgggtg accacaggaa tgtgcaaggc ggcagccggg tggcggccgg gaggcgggtg 480ggaggcgggc ggggtggcct cttcacgggc gggcctgaga gatgggcgcc cgtccggccc 540tggcgtcatc gtctccgcgt ctctacccac tgagcaaaga cacacgaaat gaagctcgaa 600cgagcacagc caaagaacgg ccgtttctgt cctttcttct taatcccttt ggcttagggt 660ttcccggcct ggacagcctg cccaagggca catgggcatc cgtccgggga cattcaggca 720gtgaccaatc ccaggccacc caggctgtgc cctgcgtcgt gggccatttc ccagccggcc 780agagatggag cagccactgc gggtccccga gtctcggtga gacagtcaag gatggacctt 840ggatggagac cggcgtgcgg ccatgtccgt gggtgaagga ggcgtgcagg ccgtgctggg 900ggacatggtt gctgtcccct cggccaaacc atgaaaagca gccctctccc ccaaccccca 960gcaccaaccc ggagaccacc ctcggccgga gcccagcacg gccaccgtca cgtctcggtc 1020gtccagcttg ggacaggtca gttcccagat gtccaggctg gagctggtcc ttgaagatcc 1080taggggtcca gcccagcaca ggagggccag gtgagagccc cctgtggttc taaggatgca 1140accaggggcc ggcggggtgc ctgccctaga gggggtaact cggccccctg gggaccagtc 1200accccaggag gtccccagag cccagctcgg agggccacag gtgcccagag tcccacctgg 1260ggaaggctgc ccctcctgcc agcccccgag ccgggcccct ggcgcccgcg tccagccgcg

1320accccgggga gatattcacc ccctgccccc gtgaatcagg aggccccgag cccatgtttt 1380cagtcctttt cctcccatcc cagcccccca ggagaagagg tgctgaactg ggtcccctgg 1440aggctcctga gccccagaac agtgccctct gagcagacgg gcactctcag accagctcac 1500gctggacaag tcagctcctg cctgccgcct gatgggccct tgggagaagc agacatggtg 1560aggaaaaggc ccctgtgccc ttcaccctaa ttccccagcc ccaagtccca ctgggttgcc 1620agcttcaacc taagcaaata attcgtgccc tctaaacaaa cgcgcgggaa tcccacctgc 1680ccttcccccg cccgcccccc cacccctggc cttgacctcc aaaagcactt gagggggctt 1740tctccagaca ccctccaacc ccgaccccat gaagaagggg tgatggggct gttaccccaa 1800caagcaagag aacgaagccc agagaggagt tggcgtggac agcaggggtc aggccccttt 1860gccccgaggg cagggctggt gccacctggg tcaggcggca ggccctggaa aagcaccgga 1920aatgagcaca cctgggtctc tagaaggttc ttccagacct ctgggggctg agtcatttca 1980acactcctgg gccgggcagg gcttcttctt ggccccgagg gacaaggtcc ccttcgtccg 2040gggggtacgg cccctggacc cctgtccccc gcaccccacc ctccgcctgg tgagggccgc 2100ggccagctct ggacacagat ccctcagagc cccttctccc tccctgctcc ctcgtcttcc 2160caagatgccc cggcctccag gtggggcagc caggcggcag aatgtggtcc aggcctctcg 2220gccccaccca cacccccctg ctctgccctg acagcctcca agacgcaggc acgtcgctgc 2280gttctgcgtc ctgtctcctc atggcacaaa acggtgcccg cctagcttcc cccagagaag 2340ggagatcgtg ctccccggac ggaccctgct ctgcctgtcc tcccgcccgg ccttcagggc 2400ctctccccaa gggtggccgc gaggaggccc tcgcctccgg ccacgggggc tccatcctcc 2460cgagcccgac aggcctccgc ctggtggtcc gacctcttcc ccaaggcccc gcccatcctc 2520ctcgcgctcc cccaaaccct gcctctttcc ccagcgccct tgtccccacg gaagaccctc 2580cacccgtgcc attacacgct ctcgccccac cctcccagcc accccccctt ccccatcctc 2640ctggaagctc ccacttcctt cccgtctccc acggcagcag agggtcagca gctcaggggt 2700cctggggccg tggagatggc ctgcccgggg gtctcgctga ccgcctccta cggaagctgt 2760gccggggggt gggggtgtct ctgcccgaac ggctggagga cgagccacat cccagggcag 2820ccggaacctg cgtcctggtc tgagacggag aggctgggtg caggtggctg aggggcctgc 2880acacagcttg gcctggggtc ccctaggtga caacactggc tgaacactca ttgctgctcc 2940ccttccaggg tgaccctggg gtccccgtgt ggccctcagg gcacacgggg gccccaccag 3000gcctcacaga accccagtgg gactgcaccc agggcccaca gaagtgcggg ggcactgggg 3060gtccagaaac aaccccacaa ccaggccaag gtggccaagg ccttactcga gcggggctgc 3120ccgtcccaag agactctggc cagtcgtccg gatccagctt cccggggccg ggccgcccgc 3180tgggctccag gcggttctgg ggggccctcc cccgggggtt cgccctccgc tctcagcagc 3240aggaagagga gcgcggccag cggatgggga gaagagggcg ccctggccat cttgctcccc 3300ctgggacttg aggagggtct cgggccgggc aggcgggacc gggagccaca gagaccctgg 3360aggaggcagc atggcgggga ggtgaccggg gaagagggcc gtgtcccagg ctcacagccc 3420ggcctggccg cccggccctc gggaggcgtg ccgctgaccg cctggccggg aggtttgctg 3480cgtgtggggt ttgcagaaag tgctgagctg ctgagcccac aggccaggct cagaggggac 3540aggaaggagg ttgctgccca gcctcgggca ctgctgaccc atctcccgtt tccagggcac 3600cagagccacc taatctgccg gctctgtgcc cagggacagg cttgcctgat ctctcaaggc 3660cgggcgctcc gccttccctg ggagagggtt aaacatccag ccccagccag catctcgggc 3720aggttcctgg ctccccccgc tcgtgcctcc tctgagaccc tggtcggcac acctttccct 3780tgagaggagg aggaggagga aagcggatgg aaccagtgac cctgcagccc ctgagggcac 3840cttcccacgt gcccccgccc gccccgcgtc ctccgccccc agttctcacg gccccagtcc 3900tgatggaggg agggcgacct ccgggctccc tggctcccgc cggctccgga agacagggcc 3960gctcggctgg ggctgcaggg aggggcccga gacgcaggag agcagcccgg aggcaaaccc 4020cgcgggtctt ccagaaggag gcctggcagg gggagggggg tgccaccact gctgtccctc 4080tcgtgccaca gtggagggtg tgggtgggca gtgccggggt gggaagtgca gaaagaccct 4140ggaccgtggg gctgggccgc cacgggggag cggggtctgt cagggaccct gggggaggga 4200ggcgaagggc tggggcagag gccggatcac ttccagattt gctgtgggac caagggccgg 4260acctcggggt gacttctttt gtgtgctggc cacagggggg ccccggcgag gtcacacgga 4320agggggcttc ggacctggcc taacaagccc actcccgagg aagatgcaag gggaggcaga 4380cggaagggcc gaagggggcg atcgggggac accgcggcag ggccggggca gagaagggag 4440gcagagggca gagaagggag gcagagggca gagaagggag gcagaggggc cacatgcttg 4500gagggccagg gaggagcggg aacggcgtcc ggcgtccagc gccgaatcag gcccgtcagg 4560cggagggtgc gtggacctgc ctggccttca cgagcacagt cagcaggctg tctcttatac 4620acatctcaac catcat 4636124482DNASus scrofa 124agcaatgggg ccgtgaccta aggaggcagg cccaggtcag tggggtgacc tctcgtggcc 60ccgatgtttg gaaatcccca aatcaaaatg acccatccga caagcttgca tgcctgcagg 120tcgactctag aggatccccg ggtaccgagc tcgaattcgc cctatagtga gtcgtattac 180aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 240aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 300gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt 360ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc 420tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgaacccc 480tt 48212517DNASus scrofa 125ggatccagcc agcagcc 1712617DNASus scrofa 126ggatccagcc tgcagcc 1712717DNASus scrofa 127acaaccccct cccacag 1712818DNASus scrofa 128acaacccccc tcccacag 1812917DNASus scrofa 129tgcggagggg agacctg 1713017DNASus scrofa 130tgcggagggg ggacctg 1713120DNASus scrofa 131aggtcaaggc caggtcgagg 201329DNASus scrofa 132aggtcgagg 913315DNASus scrofa 133ccccccccac gccgc 1513415DNASus scrofa 134cccccccccc gccgc 1513515DNASus scrofa 135cccaccccac gccgc 1513615DNASus scrofa 136cccacccccc gccgc 1513715DNASus scrofa 137cgccgcagca ggccg 1513815DNASus scrofa 138cgccgcagca agccg 1513915DNASus scrofa 139gcttatgggg ccggg 1514015DNASus scrofa 140gcttatggag ccggg 1514115DNASus scrofa 141cacggcttcg gagca 1514215DNASus scrofa 142cacggcttca gagca 1514315DNASus scrofa 143cacggcctcg gagca 1514415DNASus scrofa 144cacggcctca gagca 1514514DNASus scrofa 145gtctgcagga ggtg 1414615DNASus scrofa 146gtctgcgggc aggtg 1514715DNASus scrofa 147caagcccggg cggtt 1514815DNASus scrofa 148caagcccggt cggtt 1514915DNASus scrofa 149acctcaaggc cccca 1515015DNASus scrofa 150acctcgaggc cccca 1515115DNASus scrofa 151gccgggccca gccgc 1515215DNASus scrofa 152gctgggccca gccgc 1515315DNASus scrofa 153accagctgcg ttccc 1515415DNASus scrofa 154accagctgtg ttccc 1515515DNASus scrofa 155ggccctctgg gcgcc 1515615DNASus scrofa 156ggcgctctgg gcgcc 1515715DNASus scrofa 157gggggcgtcc cggga 1515815DNASus scrofa 158gggggtgtcc cggga 1515915DNASus scrofa 159gcggtcgggg gagtt 1516015DNASus scrofa 160gcggttgggg gagtt 1516115DNASus scrofa 161cgccccggtc ccgct 1516215DNASus scrofa 162cgccctggtc ccgct 1516315DNASus scrofa 163tcccgtctgc cggcc 1516415DNASus scrofa 164tcccatctgc cggcc 1516515DNASus scrofa 165gatgccccat ccccc 1516615DNASus scrofa 166gaagccccat ccccc 1516715DNASus scrofa 167ggcggctgct gcggc 1516815DNASus scrofa 168ggtggctgct gcggc 1516915DNASus scrofa 169tggctgcggt ctggg 1517015DNASus scrofa 170tggctgcagt ctggg 1517115DNASus scrofa 171gcgcagtgat tggca 1517215DNASus scrofa 172gcgcattgat tggca 1517314DNASus scrofa 173cgcccccccc cggg 1417414DNASus scrofa 174cgcccccccc ccgg 1417515DNASus scrofa 175cgcccccccc ccggg 1517615DNASus scrofa 176cgcccccccc cccgg 1517715DNASus scrofa 177gcagccggct cctgg 1517815DNASus scrofa 178gcagccggct cttgg 1517915DNASus scrofa 179gttgttgctc tggga 1518015DNASus scrofa 180gttgttgttc tggga 1518122DNASus scrofa 181atatatatat atatatatat at 2218270DNASus scrofa 182gtgtgtgtgt gtgtgtgcac gcgtgtgcgt gtgtacgtgt gtgtgtgtgt gtgtgtgtgt 60gtgtgtgtgt 7018338DNASus scrofa 183cacacacaca cacacacaca cacacacaca cacacaca 38

Claims

1. A method for selecting a domestic animal for having desired genotypic properties, the method comprising:testing the domestic animal for the presence of a parentally imprinted quantitative trait locus (QTL).

2. The method according to claim 1, further comprising:testing a nucleic acid sample from the domestic animal for the presence of QTL.

3. The method according to claim 1, wherein the domestic animal is a pig and the QTL is located at chromosome 2.

4. The method according to claim 2, wherein the QTL is mapping at around position 2p1.7.

5. The method according to claim 1, wherein the QTL is related to the potential muscle mass and/or fat deposition of the domestic animal.

6. The method according to claim 5 wherein the QTL comprises at least a part of an insulin-like growth factor-2 (IGF2) gene.

7. The method according to claim 3 wherein in the pig the QTL comprises a marker characterized as nt241(G-A) or as Swc9, as identified in FIG. 4.

8. The method according to claim 1, wherein a paternal allele of the QTL is predominantly expressed in the domestic animal.

9. The method according to claim 1, wherein a maternal allele of the QTL is predominantly expressed in the domestic animal.

10. An isolated and/or recombinant nucleic acid sequence comprising a parentally imprinted quantitative trait locus (QTL) or functional fragment derived thereof.

11. An isolated and/or recombinant nucleic acid sequence comprising a synthetic parentally imprinted quantitative trait locus (QTL) derived from at least one chromosome or functional fragment derived therefrom.

12. The isolated and/or recombinant nucleic acid sequence of claim 10, at least partly derived from a Sus scrofa chromosome.

13. The isolated and/or recombinant nucleic acid sequence of claim 12, wherein the isolated and/or recombinant nucleic acid sequence is at least partly derived from a Sus scrofa chromosome 2.

14. The isolated and/or recombinant nucleic acid sequence of claim 10, wherein the QTL is related to the potential muscle mass and/or fat deposition of the animal.

15. The isolated and/or recombinant nucleic acid sequence of claim 10, wherein the QTL comprises at least a part of a insulin-like growth factor-2 (IGF2) gene.

16. The isolated and/or recombinant nucleic acid sequence of claim 10, wherein a paternal allele of the QTL is capable of being predominantly expressed.

17. The isolated and/or recombinant nucleic acid sequence of claim 10, wherein a maternal allele of the QTL is capable of being predominantly expressed.

18. A method for selecting a domestic animal for having desired genotypic properties, the method comprising:testing the domestic animal for the presence of a parentally imprinted quantitative trait locus (QTL) with an isolated and/or recombinant nucleic acid sequence comprising a QTL or functional fragment derived therefrom.

19. The method according to claim 18, to select a non-human animal having desired genotypic or potential phenotypic properties.

20. The method according to claim 19, wherein the desired genotypic or potential phenotypic properties are related to muscle mass, fat deposition, lean meat, lean back fat, sow prolificacy and/or sow longevity.

21. The method according to claim 20, wherein desired the genotypic or potential phenotypic properties include sow prolificacy including phenotypic expressions selected from the group consisting of teat number, number of piglets born alive, litter size, number of total born, number of weaned piglets, and combinations of any thereof.

22. The method according to claim 20, wherein desired the genotypic or potential phenotypic properties include sow longevity includes phenotypic expressions selected from the group consisting of parity, average number of cycles per sow, and combinations thereof.

23. A non-human animal selected by the method according to claim 18.

24. The non-human animal of claim 23, wherein the non-human animal is homozygous for an allele at a paternally imprinted QTL.

25. The non-human animal of claim 23, wherein the QTL is related to the potential muscle mass and/or fat deposition of the pig and/or wherein the QTL comprises at least a part of a insulin-like growth factor-2 (IGF2) allele.

26. A non-human animal, which is transgenic, the non-human animal comprising the nucleic acid of claim 11.

27. The non-human animal of claim 23, which is a male.

28. The non-human animal of claim 23, which is a female.

29. A sperm or an embryo from the non-human animal of claim 23.

30. In a method of breeding animals destined for slaughter, the improvement comprising:utilizing the sperm or embryo of claim 29 to breed the animals.

31. The isolated and/or recombinant nucleic acid sequence of claim 13, wherein the isolated and/or recombinant nucleic acid sequence is at least partly derived from a Sus scrofa chromosome 2 from a region mapping at around position 2p1.7.

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