Patent application number | Description | Published |
20080248771 | CALIBRATION TECHNIQUES FOR FREQUENCY SYNTHESIZERS - In one embodiment, this disclosure describes a frequency synthesizer for use in a wireless communication device, or similar device that requires precision frequency synthesis but small amounts of noise. In particular, the frequency synthesizer may include a phase locked loop (PLL) and an integrated voltage controlled oscillator (VCO). The frequency synthesizer may implement one or more calibration techniques to quickly and precisely calibrate the VCO. In this manner, the analog gain of the VCO can be significantly reduced, which may improve performance of the wireless communication device. Also, the initial state of the PLL may be improved to reduce lock time of the PLL, which may enhance performance of the wireless communication device. | 10-09-2008 |
20080284508 | OUTPUT CIRCUITS WITH CLASS D AMPLIFIER - Output circuits using pulse width modulation (PWM) and/or pulse density modulation (PDM) are described. In one aspect, a PWM output circuit includes a PWM modulator that operates based on a square wave signal instead of a sawtooth or triangular wave signal. In another aspect, a PDM output circuit includes a PDM modulator that uses variable reference voltages to reduce variations in switching frequency. In yet another aspect, a dual-mode output circuit supports both PWM and PDM and includes a pulse modulator and a class D amplifier. The pulse modulator performs PWM on an input signal if a PWM mode is selected and performs PDM on the input signal if a PDM mode is selected. The class D amplifier receives a driver signal from the pulse modulator and generates an output signal. | 11-20-2008 |
20090161588 | SYSTEMS AND METHODS FOR CONTROLLING THE VOLTAGE OF SIGNALS USED TO CONTROL POWER AMPLIFIERS - A method for controlling the voltage of signals used to control power amplifiers is described. A first multiplexer and a second multiplexer are set to an enabling signal. The first multiplexer is on a first integrated circuit and the second multiplexer is on a second integrated circuit. A command is written to the first multiplexer to set the first multiplexer to one of a plurality of control signals used to control a power amplifier. A command is written to the second multiplexer to select one of the plurality of control signals that maps to the first multiplexer. The second integrated circuit is connected to a power supply. | 06-25-2009 |
20100064074 | SINGLE WIRE BUS INTERFACE - Embodiments disclosed herein address the need for a single wire bus interface. In one aspect, a device communicates with a second device via a single wire bus using a driver for driving the bus with a write frame comprising a start symbol, a write indicator symbol, an address, and data symbols. In another aspect, the device receives one or more data symbols on the single wire bus during a read frame. In yet another aspect, a device communicates with a second device via a single wire bus using a receiver for receiving a frame on the single wire bus comprising a start symbol, a write indicator symbol, an address, and one or more data symbols, and a driver for driving return read data associated with the address when the write indicator identifies a write frame. Various other aspects are also presented. These aspects provide for communication on a single wire bus, which allows for a reduction in pins, pads, or inter-block connections between devices. | 03-11-2010 |
20110105070 | Direct conversion receiver architecture - A direct downconversion receiver architecture having a DC loop to remove DC offset from the signal components, a digital variable gain amplifier (DVGA) to provide a range of gains, an automatic gain control (AGC) loop to provide gain control for the DVGA and RF/analog circuitry, and a serial bus interface (SBI) unit to provide controls for the RF/analog circuitry via a serial bus. The DVGA may be advantageously designed and located as described herein. The operating mode of the VGA loop may be selected based on the operating mode of the DC loop, since these two loops interact with one another. The duration of time the DC loop is operated in an acquisition mode may be selected to be inversely proportional to the DC loop bandwidth in the acquisition mode. The controls for some or all of the RF/analog circuitry may be provided via the serial bus. | 05-05-2011 |
20110285463 | OUTPUT CIRCUITS WITH CLASS D AMPLIFIER - Output circuits using pulse width modulation (PWM) and/or pulse density modulation (PDM) are described. In one aspect, a PWM output circuit includes a PWM modulator that operates based on a square wave signal instead of a sawtooth or triangular wave signal. In another aspect, a PDM output circuit includes a PDM modulator that uses variable reference voltages to reduce variations in switching frequency. In yet another aspect, a dual-mode output circuit supports both PWM and PDM and includes a pulse modulator and a class D amplifier. The pulse modulator performs PWM on an input signal if a PWM mode is selected and performs PDM on the input signal if a PDM mode is selected. The class D amplifier receives a driver signal from the pulse modulator and generates an output signal. | 11-24-2011 |
20110285472 | OUTPUT CIRCUITS WITH CLASS D AMPLIFIER - Output circuits using pulse width modulation (PWM) and/or pulse density modulation (PDM) are described. In one aspect, a PWM output circuit includes a PWM modulator that operates based on a square wave signal instead of a sawtooth or triangular wave signal. In another aspect, a PDM output circuit includes a PDM modulator that uses variable reference voltages to reduce variations in switching frequency. In yet another aspect, a dual-mode output circuit supports both PWM and PDM and includes a pulse modulator and a class D amplifier. The pulse modulator performs PWM on an input signal if a PWM mode is selected and performs PDM on the input signal if a PDM mode is selected. The class D amplifier receives a driver signal from the pulse modulator and generates an output signal. | 11-24-2011 |
20140210482 | METHOD AND APPARATUS FOR REDUCING COMMON MODE VARIATIONS IN A VOLTAGE SENSING COMPONENT - A method and apparatus that reduce common mode variations experienced by a voltage sensing component. A measurement component such as a BATFET or an external sensing resistor, receives, at its source, a voltage from the top of a battery having a voltage VPH_PWR. A voltage sensing component, such as an ADC, is powered by the voltage from the battery. A power referenced component, such as a power referenced LDO, tracks the voltage from the battery and outputs the tracked voltage minus a predetermined voltage amount to a negative side of the voltage sensing component. | 07-31-2014 |
Patent application number | Description | Published |
20090209029 | HIGH-DENSITY ION TRANSPORT MEASUREMENT BIOCHIP DEVICES AND METHODS - The present invention includes biochips for the measurement of cellular ion channels and methods of use and manufacture. The biochips of the present invention have enhanced sealing capabilities provided in part by chemically modifying the surface of the biochip surface or substrate or by exposure to an ionized gas. The present invention also includes novel cartridges for biochips. | 08-20-2009 |
20130266490 | HIGH-DENSITY ION TRANSPORT MEASUREMENT BIOCHIP DEVICES AND METHODS - The present invention includes biochips for the measurement of cellular ion channels and methods of use and manufacture. The biochips of the present invention have enhanced sealing capabilities provided in part by chemically modifying the surface of the biochip surface or substrate or by exposure to an ionized gas. The present invention also includes novel cartridges for biochips. | 10-10-2013 |
20140073536 | METHODS AND COMPOSITIONS FOR DETECTING NON-HEMATOPOIETIC CELLS FROM A BLOOD SAMPLE - The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell. | 03-13-2014 |
20150079677 | METHODS AND COMPOSITIONS FOR SEPARATING RARE CELLS FROM FLUID SAMPLES - The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components. | 03-19-2015 |
20160040232 | METHODS AND COMPOSITIONS FOR DETECTING NON-HEMATOPOIETIC CELLS FROM A BLOOD SAMPLE - The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell. | 02-11-2016 |
Patent application number | Description | Published |
20090279471 | LOCAL AND WIDE-AREA TRANSMISSIONS IN A WIRELESS BROADCAST NETWORK - To broadcast different types of transmission having different tiers of coverage in a wireless broadcast network, each base station processes data for a wide-area transmission in accordance with a first mode (or coding and modulation scheme) to generate data symbols for the wide-area transmission and processes data for a local transmission in accordance with a second mode to generate data symbols for the local transmission. The first and second modes are selected based on the desired coverage for wide-area and local transmissions, respectively. The base station also generates pilots and overhead information for local and wide-area transmissions. The data, pilots, and overhead information for local and wide-area transmissions are multiplexed onto their transmission spans, which may be different sets of frequency subbands, different time segments, or different groups of subbands in different time segments. More than two different types of transmission may also be multiplexed and broadcast. | 11-12-2009 |
20110019632 | METHOD AND APPARATUS FOR RANKING OF MEDIA SERVICES AND PROGRAM PACKAGES - A method for improved presentation of media information for wireless communication is described herein. Media services and program packages are ranked and ranking information is delivered to a mobile device along with the media information. The ranking information is then used by the mobile device to arrange the media services in a ranked order. The ranking information can also be used to assign a default service that is displayed whenever the mobile device is activated or whenever a selection is made to view media content. The ranking information can also be used to arrange program packages in a ranked order for subscription purposes. | 01-27-2011 |
20110075638 | TIME SLICING TECHNIQUES FOR VARIABLE DATA RATE ENCODING - The disclosure is directed to a multi-channel encoder. The multi-channel encoder is configured to generate an encoded data stream. The multi-channel encoder includes a plurality of channel encoders, and a processor configured to allocate time slots in the encoded data stream to each of the channel encoders to vary the rate of data provided by each of the channel encoders into the encoded data stream. | 03-31-2011 |
20110299587 | METHODS AND SYSTEMS FOR RESIZING MULTIMEDIA CONTENT BASED ON QUALITY AND RATE INFORMATION - In general, this disclosure describes techniques for exchanging information between a plurality of encoder modules and a multiplex module to combine segments of data from the encoder modules with an improved overall quality. In particular, the encoder modules associate their respective segments of data with quality and rate information and send at least the quality and rate information associated with the segments of data to the multiplex module. The multiplex module analyzes the quality and rate information to determine whether the segments of data that encoder modules desire to include in the current superframe fit within the available bandwidth of a transmission channel. If the multiplex module determines the plurality of segments of data do not fit within the available bandwidth, the multiplex module selects one or more of the segments to be resized based on the quality and rate information received from the encoder modules. | 12-08-2011 |
20120294360 | CHANNEL SWITCH FRAME - Methods and apparatus to process multimedia data enabling faster channel acquisitions, improved error recovery and improved efficiency. An encoder device encodes a first portion of multimedia data using inter-coding to generate a first version, and encodes the first portion of multimedia data using intra-coding to generate a second version. A decoder device receives a first version of a first portion of multimedia data, wherein the first version is inter-coded, receives a second version of the first portion of multimedia data, wherein the second version is intra-coded, and selectively decodes the first and second received versions. | 11-22-2012 |
20130021204 | APPARATUS AND METHOD FOR WIRELESS NETWORK ENHANCEMENT VIA VARIABLE DOWN TILT - Apparatus and methods for controlling antenna down tilt in a mixed coordinated/non-coordinated network include receiving one or more input signals defining a waveform to be transmitted, for determining a tilt angle state to be applied to the antenna based on the one or more input signals, and for transmitting a tilt control signal if the determined tilt angle state differs from a current tilt angle state associated with the antenna. | 01-24-2013 |
20130163458 | METHODS AND APPARATUS FOR POSITION LOCATION IN A WIRELESS NETWORK - Methods and apparatus for position location in a wireless network. In an aspect, a method is provided that includes determining whether a symbol to be transmitted is an active symbol, wherein the symbol comprises a plurality of subcarriers, and encoding identification information on a first portion of subcarriers if it is determined that the symbol is the active symbol. The method also includes encoding idle information on a second portion of subcarriers if it is determined that the symbol in not the active symbol. In an aspect, an apparatus includes detector logic configured to decode a plurality of symbols to determine identification information that identifies a plurality of transmitters, and to determine a plurality of channel estimate associated with the plurality of transmitters. The apparatus also includes position determination logic configured to calculate a device position based on the plurality of transmitters and the plurality of channel estimates. | 06-27-2013 |
20130308707 | METHODS AND DEVICE FOR DATA ALIGNMENT WITH TIME DOMAIN BOUNDARY - Apparatus and methods of using content information for encoding multimedia data are described. A method of processing multimedia data includes obtaining content information of multimedia data, and encoding the multimedia data so as to align a data boundary with a frame boundary in a time domain, wherein said encoding is based on the content information. In another aspect, a method of processing multimedia data includes obtaining a content classification of the multimedia data, and encoding blocks in the multimedia data as intra-coded blocks or inter-coded blocks based on the content classification to increase the error resilience of the encoded multimedia data. Apparatus that can process multimedia data described in these methods are also disclosed. | 11-21-2013 |
20140016491 | METHODS AND APPARATUS FOR REPORTING SIGNAL QUALITY IN OVERLAPPING MULTIMEDIA BROADCAST SINGLE FREQUENCY NETWORK (MBSFN) AREAS - Certain aspects of the present disclosure relate to methods and apparatus for reporting signal quality in overlapping Multimedia Broadcast Single Frequency Networks (MBSFN) areas. A UE may determine a signal quality estimate for each of two or more overlapping MBSFN areas based on Signal to Noise Ratio (SNR) information and Modulation and Coding Scheme (MCS) information for the MBSFN area. The UE may then determine a combined signal quality based on the signal quality estimates of the MBSFN areas. | 01-16-2014 |
20140036755 | MBMS SUPPORT IN HETEROGENEOUS NETWORK AND IN-VENUE - A method, an apparatus, and a computer program product for wireless communication enable user equipment operating in a current cell that provides a multimedia broadcast/multicast service to distinguish between neighboring cells that have different operational characteristics. The presence of a neighboring cell is identified while the user equipment is operating in a first cell and it is determined whether the neighboring cell provides services different from the services provided in the current cell, based on information maintained by the user equipment. The user equipment may move to the neighboring cell to obtain better or different service. | 02-06-2014 |
20140044094 | Multiplexing and Transmission of Multiple Data Streams in a Wireless Multi-Carrier Communication System - Techniques for multiplexing and transmitting multiple data streams are described. Transmission of the multiple data streams occurs in “super-frames”. Each super-frame has a predetermined time duration and is further divided into multiple (e.g., four) frames. Each data block for each data stream is outer encoded to generate a corresponding code block. Each code block is partitioned into multiple subblocks, and each data packet in each code block is inner encoded and modulated to generate modulation symbols for the packet. The multiple subblocks for each code block are transmitted in the multiple frames of the same super-frame, one subblock per frame. Each data stream is allocated a number of transmission units in each super-frame and is assigned specific transmission units to achieve efficient packing. A wireless device can select and receive individual data streams. | 02-13-2014 |
20140153471 | ALLOWING UNICAST SUBFRAME STRUCTURE FOR EMBMS - A method, an apparatus, and a computer program product for wireless communication are provided. The apparatus receives a multicast/broadcast single frequency network (MBSFN) subframe configured based on a unicast subframe structure, and transmits MBSFN signals for eMBMS using the MBSFN subframe. In a one configuration, the MBSFN subframe structure for eMBMS transmissions uses the same CP length, the same common reference signal (CRS) pattern and same subframe structure used for unicast, along with the same antenna ports used for unicast transmission. In another configuration, the MBSFN subframe structure for eMBMS transmissions uses the same CP length and same subframe structure used for unicast, but potentially different CRS patterns and different antenna ports from those used for unicast transmissions. In another configuration, the MBSFN subframe structure for eMBMS transmission uses the same CP length and same subframe structure used for unicast, but with a UE-RS pattern. | 06-05-2014 |
Patent application number | Description | Published |
20090205064 | Mutated Acetohydroxyacid Synthase Genes in Brassica - Provided are mutated acetohydroxyacid synthase (AHAS) nucleic acids and the proteins encoded by the mutated nucleic acids. Also provided are canola plants, cells, and seeds comprising the mutated genes. | 08-13-2009 |
20120060243 | Herbicide-Resistant AHAS-Mutants and Methods of Use - The invention provides transgenic or non-transgenic plants with improved levels of tolerance to AHAS-inhibiting herbicides. The invention also provides nucleic acids encoding mutants of the acetohydroxyacid synthase (AHAS) large subunit, expression vectors, plants comprising the nucleic acids encoding the AHASL subunits containing one, two, or more mutations, plants comprising such AHASL subunit mutant nucleic acids, methods for making and using the same, and methods of controlling weeds. | 03-08-2012 |
20120073186 | FATTY ACID BLENDS AND USES THEREFOR - Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel. | 03-29-2012 |
20120178628 | MUTATED ACETOHYDROXYACID SYNTHASE GENES IN BRASSICA - Provided are mutated acetohydroxyacid synthase (AHAS) nucleic acids and the proteins encoded by the mutated nucleic acids. Also provided are canola plants, cells, and seeds comprising the mutated genes. | 07-12-2012 |
Patent application number | Description | Published |
20080256668 | Non-Transgenic Herbicide Resistant Plants - The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphoniorethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phophonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. The present invention also relates to a non-transgenic plant cell in which the EPSPS gene has been mutated, a non-transgenic plant regenerated therefrom, as well as a plant resulting from a cross using a regenerated non-transgenic plant having a mutated EPSPS gene. | 10-16-2008 |
20090298150 | PRODUCTION OF SQUALENE USING YEAST - Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically altered yeast and uses therefore. In some aspects and embodiments, the genetically altered yeast produce isoprenoids, preferably squalene. The genetically altered yeast may have alterations in the expression or activity of enzymes involved in squalene production. for example, acetyl-CoA carboxylase (or “ACCase”), HMG-CoA reductase, squalene epoxidase, and squalene synthase. One or more genes of a genetically altered yeast may be modified by gene repair oligonucleobases. Also are provided methods of producing squalene using a genetically altered yeast. The invention also provides squalene produced by genetically altered yeast. | 12-03-2009 |
20100058651 | FATTY ACID BLENDS AND USES THEREFOR - Provided herein are blends oils or fatty acids comprising more than 50% medium chain fatty acids, or the fatty acid alkyl esters thereof, and having low melting points. Such blends are useful as a fuel or as a starting material for the production of, for example, a biodiesel. Also provided genetically altered or modified plants, modified such that the amount of medium chain fatty acids generated by the plant are increased. Further provided is a method of predicting the melting point of a blend of fatty acid methyl esters and the use of such a method for identifying blends suitable for use as, for example, a biodiesel. | 03-11-2010 |
20100203639 | COMPOSITIONS AND METHODS FOR PLANT GENETIC MODIFICATION - Mixed duplex oligonucleotides (MDON) are used to effect site-specific genetic alterations in a target DNA sequence of a plant. The MDON are introduced by electroporation into microspores. Thereafter, plants having a desired genetic alteration are produced by germinating the microspores. | 08-12-2010 |
20110124072 | METHODS AND COMPOSITIONS FOR PRODUCING SQUALENE USING YEAST - Provided herein compositions and methods for producing isoprenoids, including squalene. In certain aspects and embodiments provided are genetically converted yeast and uses therefore. In some aspects and embodiments, the genetically converted yeast produce isoprenoids, preferably squalene. Also are provided methods of producing squalene using a genetically converted yeast or a non-genetically converted yeast. The invention also provides squalene produced by genetically converted yeast or non-genetically converted yeast. | 05-26-2011 |
20120122223 | MUTATED PROTOPORPHYRINOGEN IX OXIDASE (PPX) GENES - Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant. | 05-17-2012 |
20140134696 | METHODS AND COMPOSITIONS FOR PRODUCING SQUALENE USING YEAST - Provided herein compositions and methods for producing isoprenoids, including squalene. Is certain aspects and embodiments provided are genetically converted yeast and uses therefore. In some aspects and embodiments, the genetically converted yeast produce isoprenoids, preferably squalene. Also are provided methods of producing squalene using a genetically converted yeast or a non-genetically converted yeast. The invention also provides squalene produced by genetically converted yeast or non-genetically converted yeast. | 05-15-2014 |
20140189906 | MUTATED PROTOPORPHYRINOGEN IX OXIDASE (PPX) GENES - Provided are compositions and methods relating to gene and/or protein mutations in transgenic or non-transgenic plants. In certain embodiments, the disclosure relates to mutations in the protoporphyrinogen IX (PPX) gene. In some embodiments the disclosure relates to plants that are herbicide resistant. | 07-03-2014 |
Patent application number | Description | Published |
20110044994 | HUMAN IMMUNODEFICIENCY VIRUS (HIV)-NEUTRALIZING ANTIBODIES - The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided. | 02-24-2011 |
20140205612 | HUMAN IMMUNODEFICIENCY VIRUS (HIV)-NEUTRALIZING ANTIBODIES - The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided. | 07-24-2014 |
20150218257 | MONOCLONAL ANTIBODIES DIRECTED AGAINST TRIMERIC FORMS OF THE HIV-1 ENVELOPE GLYCOPROTEIN WITH BROAD AND POTENT NEUTRALIZING ACTIVITY - The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies are characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided. | 08-06-2015 |