Patent application number | Description | Published |
20090104616 | PRIMER SET FOR AMPLIFYING SULT1A1 GENE, REAGENT FOR AMPLIFYING SULT1A1 GENE CONTAINING THE SAME, AND THE USES THEREOF - A primer set for amplifying a region including sites to be detected of SULT1A1*2 and SULT1A1*3 in the SULT1A1 gene by a gene amplification method is provided, wherein the primer set can amplify the region specifically. A pair of primer set is used including a forward primer consisting of the base sequence of SEQ ID NO: 7 as well as a reverse primer consisting of the base sequence of SEQ ID NO: 18. The use of this primer set makes it possible to specifically and efficiently amplify, a region including both sites where two types of polymorphisms (SULT1A1*2 and SULT1A1*3) of the SULT1A1 gene are generated. | 04-23-2009 |
20090113980 | METHOD OF VERIFYING PERFORMANCE OF OPTICAL DETECTION APPARATUS AND STANDARD REAGENT USED THEREFOR - A method is provided in which with respect to an optical detection apparatus including an optical detection unit and a temperature control unit, whether optical signal detection and temperature control are performed accurately, i.e. the performance thereof, can be verified simply with high reliability. With respect to an optical detection apparatus including an optical detection unit for detecting an optical signal of a sample and a temperature control unit for controlling temperature of the sample, the optical signal detection performance and temperature control performance are verified by the following method. First, a standard sample containing a nucleic acid sequence and a strand complementary thereto that have a known optical signal intensity and Tm value is provided, the temperature of the standard sample is increased or decreased with the temperature control unit, and optical signal intensity of the standard sample is measured with the detection unit. On the other hand, the melting temperature of the standard sample is determined from a change in the optical signal intensity accompanying a change in the temperature. The measured optical signal intensity and melting temperature of the standard sample are compared to the known optical signal intensity and melting temperature of the standard sample, respectively, and thereby it is verified whether the optical signal detection performance of the detection unit and the temperature control performance of the temperature control unit are accurate. | 05-07-2009 |
20110117568 | Method for Amplifying Target Nucleic Acid Sequence, Method for Detecting Mutation Using the Same, and Reagent Used for the Same - The present invention provides a method for detecting a mutation capable of detecting a mutation with high sensitivity and high reliability in one reaction system. Using primers (Xmt) and (Xwt), a target nucleic acid sequence whose objective base to be detected is a mutant-type is amplified with amplification efficiency higher than a target nucleic acid sequence whose objective base to be detected is a normal-type. The (Xmt) is a primer that is complementary to a region including a mutant-type base in the template nucleic acid and has a base complementary to a mutant-type base at a 3′ region, and the (Xwt) is a primer that is complementary to a region including a normal-type base in the template nucleic acid and has a base complementary to a normal-type base at a 3′ region. It is preferable that amplification efficiency by the (Xmt) with reference to a mutant-type template nucleic acid is higher than that by the (Xwt) with reference to a normal-type template nucleic acid. Then, a signal value that shows a molten state of a hybridization product between the thus obtained amplification product and the probe is measured, and the presence or absence of the mutation of the objective base site is determined from a change in the signal value accompanying a change in the temperature. | 05-19-2011 |
20120009576 | Probe for Detection of Polymorphism in ABL Gene, and Use Thereof - A probe for detecting a polymorphism in ab1 gene, said probe comprising at least one fluorescence-labeled oligonucleotide selected from P1 below:
| 01-12-2012 |
20120021419 | Analyzing System, Analyzing Apparatus, Container, Analyzing Method, Program, and Recording Medium - An analyzing system that enables further expansion of analysis items and automation of analysis. In the analyzing system for performing an analysis using container | 01-26-2012 |
20120129174 | Target Sequence Amplification Method, Polymorphism Detection Method, and Reagents for Use in the Methods - An object of the present invention is to provide an amplification method that inhibits amplification caused by erroneous annealing of a primer. Primers X1 and X2 are used in amplification of a target sequence including a target site showing a polymorphism. The primer X1 includes a sequence A1′ and a sequence E1. The sequence A1′ is complementary to a partial sequence A1 in a template nucleic acid, and has, in its 3′ region, a base x1′ complementary to a first base x1 at the target site in a 5′ region of the sequence A1. The sequence E1 is noncomplementary to a partial sequence B1 adjacent to the 3′ end of the partial sequence A1 in the template nucleic acid, and is bound to the 5′ end of the partial sequence A1′. The primer X2 includes a sequence A2′. The sequence A2′ is complementary to a partial sequence A2 in the template nucleic acid, and has, in its 3′ region, a base x2′ complementary to a second base x2 at the target site in a 5′ region of the partial sequence A2. Each of the primers X1 and X2 has a base complementary to the target site in its 3′ region. By these primers, when only a template in which the target site is the first base x1 is present, erroneous amplification of the target sequence having the second base x2 can be prevented. Thus, a false positive for the polymorphism of the second base x2 can be inhibited. | 05-24-2012 |
20120244540 | Probe for Detecting Polymorphism in Disease-Related Gene and Use of the Probe - The present invention provides a polymorphism detection probe that can identify a different polymorphism in a K-ras gene easily with high reliability and use of the polymorphism detection probe. | 09-27-2012 |
20120276534 | Probe for Detecting Polymorphism in Exon 12 of NPM1 Gene and Use Thereof - The present invention relates to probes which detect a polymorphism(s) in exon 12 of the NPM1 gene, a kit therefor, and the method of detecting the polymorphism(s) thereof. | 11-01-2012 |
20130143212 | Method of Determining the Abundance of a Target Nucleotide Sequence of a Gene of Interest - The disclosure relates to a method of measuring gene abundance, including obtaining at least two types of amplification product, each of which contains a single additional base sequence and corresponds to each of at least two genes, by amplifying, in one reaction solution, nucleic acids encoding the at least two genes, whose abundances in nucleic acids contained in a subject sample may be different, using a first primer set, which includes at least two types of first primer, each of which is capable of introducing the single additional base sequence to a resulting amplification product and corresponds to each of the at least two genes, and a second primer for amplifying a nucleic acid containing the single additional base sequence; and determining the abundances of the at least two genes based on detected signals corresponding to the abundances of the at least two types of amplification product. | 06-06-2013 |
20130309663 | METHOD FOR DETECTING MUTATIONS AT IL28B AND ITPA - The disclosure relates to probes for detecting a polymorphism in the IL28B gene and in the ITPA gene, and methods of use thereof. | 11-21-2013 |