Patent application number | Description | Published |
20080260577 | CHEMILUMINESCENT DETECTION SYSTEM - Throughput is improved by increasing the number of micro-reaction chambers. There is provided a chemiluminescent detection system that has a so-called plate on which many reaction chambers are one-dimensionally or two-dimensionally arranged, characterized in that optical detection is performed using a line or area sensor having many detection pixels, the spacing of the optical detection pixels substantially matches the spacing of the reaction chambers on the plate, and the micro-reaction chambers and the pixels are made to correspond one-to-one with each other so that light from the reaction chambers on the plate enters the detection pixels most efficiently and does not scatter to other pixels. To make the micro-reaction chambers arrayed on the plate and the pixels of the image pickup element plate correspond one-to-one with each other, light-emitting substances or reflectors or photoabsorption substances are placed so as to serve as alignment marks. | 10-23-2008 |
20080317627 | CHEMILUMINESCENCE ANALYZER - The present invention aims to achieve both rapid supply of reagent substances to micro-chambers and inhibition of contamination from adjacent chambers. For achieving the above objects, the shape of a flow channel in a flow cell including a plate having micro-chambers is varied between the time of substance supply and the time of luminescence reaction. | 12-25-2008 |
20080318244 | Large-scale parallel nucleic acid analysis method - It is intended to provide a technique for amplifying, individually and in parallel, nucleic acids contained in a mixture of plural kinds of nucleic acid samples. The present invention provides a nucleic acid analysis method comprising amplification means, whereby amplification reaction is performed in a reaction solution comprising a homogeneous solvent and comprising at least plural template nucleic acids and solid phase carriers comprising one or more kinds of amplification probes immobilized on the surface, to prevent amplified products attributed to two or more template nucleic acids from being replicated in one solid phase carrier. According to the present invention, plural kinds of analyte nucleic acid samples in a mixed state can be amplified individually and in parallel. This method achieves one solid phase carrier-one nucleic acid. Therefore, a higher density of solid phase carriers with obtained amplified products is easily achieved, leading to improved throughput of amplified product analysis. Reactions in all the amplification reaction steps are performed under homogeneous solvent conditions. Therefore, the method of the present invention is performed by convenient procedures and as such, is suitable to automation. | 12-25-2008 |
20090087344 | Small size gene analysis apparatus - By the conventional technique for dispensing more than one reagents accurately, the system is complicated and thus a compact and inexpensive system is difficult to realize. In the present invention, the pressurized dispensing system utilizing a capillary is realized, and in addition, in order to reduce the leakage of reagents different from the reagent dispensed, by forming air layers at the tips of the capillaries after dispensing, a compact, simple, inexpensive analysis apparatus is realized. | 04-02-2009 |
20090215162 | Nucleic acid amplification device - This invention provides a nucleic acid amplification device whereby the abundance of a target molecule can be maintained before and after a step of separately amplifying a sample such that highly accurate analysis results that can be applied to gene expression analysis can be obtained. Also, a nucleic acid amplification device having a structure in which a plurality of minute reaction cells each comprising a set of a bead-retaining space capable of retaining a single analysis bead and a reagent reaction space retaining no bead but having a volume that is large enough to cause a reagent reaction therein are positioned so as to form a planar face is provided. | 08-27-2009 |
20110244448 | DNA DETECTING APPARATUS, DNA DETECTING DEVICE AND DNA DETECTING METHOD - A chemiluminescence detecting apparatus includes a thing called a plate where many reaction chambers are one-dimensionally or two-dimensionally arranged, and enzymes packed in a gel and DNA-immobilized beads are simultaneously charged into the reaction chambers, thereby trapping a large amount of enzymes in the reaction chambers, improving enzyme activity and achieving high sensitive pyrosequencing. | 10-06-2011 |
20120270210 | METHOD AND REAGENT FOR GENE SEQUENCE ANALYSIS - Provided is a nucleic acid substrate which has nucleic acid substrate characteristics equivalent to those of dATP, has a low substrate specificity for luciferase, exerts no negative effect on enzymatic reactions such as a complementary-strand synthesis, and therefore is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to nucleotide T, a 7-substituted deoxyribonucleotide triphosphate whose 7-position of a purine group is modified by a substituent is used as a substitute for a nucleotide α-thiotriphosphate analog. | 10-25-2012 |
20150159202 | METHODS FOR QUANTITATIVE cDNA ANALYSIS IN SINGLE-CELL - A method of synthesizing cDNA, in sequence, includes picking up a whole single-cell from a sample containing at least a single-cell having a cell membrane, lysing the cell membrane and extracting nucleic acids from the cell, degrading DNA of the extracted nucleic acids with DNase, hybridizing mRNA of the total RNA contained in the whole single-cell with oligo (dT) fixed onto a carrier, performing reverse transcription of the mRNA hybridized with the oligo (dT) to fix cDNA derived from the single-cell onto the carrier, thereby preparing a single-cell derived cDNA library fixed onto a carrier, and amplifying cDNA fixed onto the carrier. | 06-11-2015 |