Patent application number | Description | Published |
20090004666 | METHOD OF DETECTING NUCLEOTIDE SEQUENCE WITH AN INTRAMOLECULAR PROBE - A nucleotide sequence-detecting method, including preparing a first intramolecular detecting sequence having a sequence complementary to a first sequence located at a 3′-side of the detecting site contained in the nucleotide sample and a second intramolecular detecting sequence having a sequence complementary to a second sequence located at a 5′-side of the detecting site, preparing a detecting chain containing a sequence of the detecting chain by connecting the first intramolecular detecting sequence to the 3′ terminal of the nucleotide sample and the second intramolecular detecting sequence to the 5′ terminal, allowing intramolecular hybridization at two positions of the detecting chain, connecting the 3′ terminal of the first intramolecular detecting sequence to the 5′ terminal of the second intramolecular detecting sequence, obtaining a cyclic structure, detecting the desired sequence in the nucleotide sample from the cyclic structure. | 01-01-2009 |
20090075285 | PRIMER KIT - The aim of the present invention is to provide a primer kit capable of efficiently amplifying only a required target nucleotide sequence without shortage or excess in the case of amplifying a wide range of target nucleotide sequences. The present invention is directed to a primer kit having a plurality of types of primers for amplifying nucleic acids having a plurality of types of target nucleotide sequences, wherein all of the types of primers are primers capable of amplifying each nucleic acid having a target nucleotide sequence under the same reaction conditions, and a single type of primer or primers corresponding to a single target nucleotide sequence is/are housed in at least one container; and a primer kit having a plurality of types of primers for amplifying nucleic acids having a plurality of types of target nucleotide sequences, wherein all of the types of primers are primers capable of amplifying each nucleic acid having a target nucleotide sequence under the same reaction conditions, and primers corresponding to a single target nucleotide sequence are housed in the same container. | 03-19-2009 |
20100009372 | METHOD FOR ESTIMATING TARGET NUCLEIC ACID RATIO - The present invention provides a method for estimating a ratio between the amount of target nucleic acid and the amount of reference nucleic acid in the examined nucleic acid samples from the PCR reaction solutions at the endpoint. | 01-14-2010 |
20100261186 | POLYMORPHISM IDENTIFICATION METHOD - The present invention is to provide a method for identifying a polymorphism with high sensitivity and high accuracy. The method of the present invention includes: performing a nucleic acid chain extension reaction and identifying the polymorphism of the nucleic acid contained in a test nucleic acid sample. The extension reaction is conducted with use of: a nucleic acid in a test nucleic acid sample as a template, a type I detection primer which hybridizes with a region including the polymorphic site of a nucleic acid whose polymorphic site nucleotide sequence consists of a first nucleotide sequence, and a polymerase having no strand displacement activity. The reaction is conducted with the presence of an inhibitory oligonucleotide which contains a nucleotide sequence complementary to the sequence of a region including the polymorphic site of a nucleic acid whose polymorphic site nucleotide sequence consisting of a second nucleotide sequence. | 10-14-2010 |
20100267036 | POLYMORPHISM IDENTIFICATION METHOD - The present invention is to provide a method for identifying a polymorphism. The method includes performing a nucleic acid chain extension reaction and identifying the polymorphism of the nucleic acid contained in the test nucleic acid sample. The extension reaction is conducted with use of a nucleic acid in a test nucleic acid sample as a template, a type I detection primer which hybridizes with a region including the polymorphic site of a nucleic acid whose polymorphic site nucleotide sequence consisting of a first nucleotide sequence, and a polymerase. The reaction is conducted with the presence of an inhibitory oligonucleotide, which hybridizes with the type I detection primer. The region of the type I detection primer to hybridize with the inhibitory oligonucleotide is located on the 5′ side of the polymorphism detection site of the type I detection primer to hybridize with the polymorphic site. | 10-21-2010 |
20100317019 | NUCLEIC ACID DETECTION METHOD AND NUCLEIC ACID DETECTION KIT - The present invention is directed to a method with which one can detect a nucleic acid with sufficient accuracy, no matter the nucleotide length of a primer, and no matter if the analyte nucleotide consists of only a single nucleotide. Specifically disclosed is a method for detecting whether or not a nucleic acid sample contains a nucleic acid which has a target nucleotide sequence comprising one or more analyte nucleotide(s) in a nucleic acid sample. Firstly, in a step (a), a nucleic acid extension reaction is performed with use of a nucleic acid contained in the nucleic acid sample, an analyte nucleotide-identification primer, and a polymerase. Next, in a step (b), an extension product yielded from the step (a) is detected. The step (a) uses, as the primer, a polynucleotide which comprises a nucleotide sequence having an insertion site or a deletion site of one or more nucleotide(s), on the 5′ side of nucleotide(s) corresponding to the analyte nucleotide(s), in a nucleotide sequence homologous or complementary to an analyte nucleotide-containing partial region of the target nucleotide sequence. | 12-16-2010 |
20110117547 | TARGET DNA DETECTION METHOD AND TARGET DNA DETECTION KIT - The present invention provides a method for detecting a target DNA easily and highly accurately through simultaneous analysis of a sense strand and an antisense strand of the target DNA, and a kit therefor. The target DNA detection method of the present invention is a method for detecting a target DNA composed of a sense strand having a target nucleotide sequence and an antisense strand complementary to the sense strand, wherein: a first oligonucleotide which hybridizes with the sense strand, and a second oligonucleotide which hybridizes with the antisense strand are used; at least a part of a region, of the antisense strand, which hybridizes with the second oligonucleotide is complementary to at least a part of a region, of the sense strand, which hybridizes with the first oligonucleotide; and the first oligonucleotide and the second oligonucleotide are simultaneously added into one reaction solution to effect respective hybridizations with the target DNA, followed by ligation reactions and/or PCR, thereby detecting resultant products. | 05-19-2011 |
20120007540 | MOTOR CONTROL DEVICE - Provided is a motor control device which realizes automatic adjustment of control of a motor for driving a mechanical load through a simple operation. The motor control device includes: a follow-up control unit ( | 01-12-2012 |
20120318956 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS - There is provided an optical analysis technique enabling the detection of the condition or characteristic of a particle to be observed contained at a low concentration or number density in a sample solution. The inventive optical analysis technique uses an optical system capable of detecting light from a micro region in a solution, such as an optical system of a confocal microscope or a multiphoton microscope, to detect the light from the light-emitting particle to be observed while moving the position of the micro region in the sample solution (while scanning the inside of the sample solution with the micro region), thereby detecting individually the light-emitting particle crossing the inside of the micro region to enable the counting of the light-emitting particle(s) or the acquisition of the information on the concentration or number density of the light-emitting particle. | 12-20-2012 |
20120319009 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS - The inventive optical analysis technique uses an optical system capable of detecting light from a micro region in a solution, such as an optical system of a confocal microscope or a multiphoton microscope, to detect the light from the light-emitting particle to be observed while moving the position of the micro region in the sample solution (while scanning the inside of the sample solution with the micro region); generates time series light intensity data, computes a characteristic value of the light intensity indicating the presence or absence of the light from a single light-emitting particle in every time section of a predetermined width in the light intensity data; and detects the light-emitting particle crossing the inside of the micro region individually using the characteristic value, thereby enabling the counting of the light-emitting particle(s) or the acquisition of the information on the concentration or number density of the light-emitting particle. | 12-20-2012 |
20130048875 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS - The inventive optical analysis technique uses an optical system capable of detecting light from a micro region in a solution, such as an optical system of a confocal microscope or a multiphoton microscope, to detect the light from the light-emitting particle to be observed while moving the position of the micro region in the sample solution (while scanning the inside of the sample solution with the micro region); generates time series light intensity data, and after smoothing the time series light intensity data; and detects in the smoothed time series light intensity data the light-emitting particle crossing the inside of the micro region individually, thereby enabling the counting of the light-emitting particle(s) or the acquisition of the information on the concentration or number density of the light-emitting particle. | 02-28-2013 |
20130228705 | OPTICAL ANALYSIS METHOD USING THE LIGHT INTENSITY OF A SINGLE LIGHT-EMITTING PARTICLE - There is provided a scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope, enabling characterization of a light-emitting particle or identification of a light-emitting particle with emitted light intensity of a single light-emitting particle measured individually. In the inventive optical analysis technique, with reference to the ratio of the intensities of simultaneously generated signals of the lights of at least two light-emitting sites having mutually different emission wavelengths, possessed by a light-emitting particle contained in a sample solution, the intensities being measured with moving the position of the light detection region of an optical system by changing the optical path of the optical system, a single light-emitting particle corresponding to the signals is identified, and the kind, the size, etc. of the light-emitting particle is identified. | 09-05-2013 |
20130230874 | OPTICAL ANALYSIS METHOD USING MEASUREMENT OF LIGHT OF TWO OR MORE WAVELENGTH BANDS - There is provided an optical analysis technique enabling identification of a kind of light-emitting particle corresponding to a signal on a time series light intensity data or identification of a signal corresponding to light-emitting particles other than a particle to be observed in an optical measurement using a confocal microscope or a multiphoton microscope. The inventive optical analysis technique measures simultaneously and separately intensities of lights of two or more wavelength bands from a light detection region in a sample solution containing light-emitting particles of two or more kinds to generate time series light intensity data of the respective wavelength bands; detects signals simultaneously generated on the time series light intensity data of at least two wavelength bands; and identifies the simultaneously generated signals as signals of a light-emitting particle of at least one specific kind. | 09-05-2013 |
20130242307 | OPTICAL ANALYSIS DEVICE AND OPTICAL ANALYSIS METHOD USING A WAVELENGTH CHARACTERISTIC OF LIGHT OF A SINGLE LIGHT-EMITTING PARTICLE - The inventive technique of detecting and analyzing light from a light-emitting particle in accordance with the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope is characterized by detecting intensities of components of two or more wavelength bands of light from a light detection region of an optical system with moving the position of the light detection region in a sample solution by changing the optical path of the optical system of the microscope; detecting individually signals of the light from each light-emitting particle in the intensities of the components of the two or more wavelength bands of the detected light; and identifying a kind of light-emitting particle based on the intensities of the components of the two or more wavelength bands of the signals of the light of the detected light-emitting particle. | 09-19-2013 |
20130302906 | OPTICAL ANALYSIS METHOD AND OPTICAL ANALYSIS DEVICE USING THE DETECTION OF LIGHT FROM A SINGLE LIGHT-EMITTING PARTICLE - In the scanning molecule counting method using the light measurement with a confocal microscope or a multiphoton microscope, a measuring time is optimized with suppressing the scattering in a result small irrespective of light-emitting particle concentrations. In the inventive technique of detecting and analyzing the light from an light-emitting particle, there are repeated processes of detecting the light intensity from a light detection region with moving the position of the light detection region of an optical system in a sample solution by changing the optical path of the optical system of the microscope, and detecting the signals of the light of light-emitting particles individually, and based on the time taken for the number of the signals from the light-emitting particles to reach a predetermined number, the light-emitting particle concentration in the sample solution is determined. | 11-14-2013 |
20130314705 | METHOD OF MEASURING A DIFFUSION CHARACTERISTIC VALUE OF A PARTICLE - There is provided a method of measuring a diffusion characteristic value (for example, a diffusion constant) of a light-emitting particle using the scanning molecule counting method using the optical measurement with a confocal microscope or a multiphoton microscope. The inventive method of measuring a diffusion characteristic value of a light-emitting particle is characterized to measure light intensity from the light detection region with moving the position of the light detection region in the sample solution by changing an optical path of the optical system to generate light intensity data and to compute a diffusion characteristic value of the light-emitting particle based on a deviation time from a moving cycle time of the light detection region in an interval of generation times of two or more signals corresponding to a same light-emitting particle on the light intensity data. | 11-28-2013 |
20130338968 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided a structure to make the setting of a criterion for eliminating noises easy in the scanning molecule counting method. In the inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution, time series light intensity data of light from a light detection region detected with moving the position of the light detection region in the sample solution is generated, and a signal of a light-emitting particle individually is detected in the time series light intensity data, wherein a signal having a light intensity in a light intensity range set based upon a signal generation frequency integrated value distribution which is a distribution, obtained by using as a variable an intensity of a signal, of integrated values of generation frequencies of signals having an intensity not lower than the variable is extracted as the signal of the light-emitting particle. | 12-19-2013 |
20140024020 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided a structure to reduce the size of light intensity data in the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope. In the inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution, time series light intensity data of light from a light detection region detected with moving the position of the light detection region of the microscope in the sample solution is generated, and a signal of a light-emitting particle individually is detected in the time series light intensity data. In that case, regions where no signal indicating light of light-emitting particles exist in the time series light intensity data is removed from the time series light intensity data. | 01-23-2014 |
20140131593 | METHOD FOR DETECTING FLUORESCENT PARTICLES - A method for detecting a fluorescent particle comprises the preparation of a sample solution containing fluorescent particles and a substance that promotes transition of the fluorescent particles from a triplet excited state to a singlet ground state, and calculation of the number of molecules of fluorescent particles present in the prepared sample solution. Calculation of the number of molecules of the fluorescent particles comprises moving the location of a photodetection region of an optical system in the sample solution using the optical system of a confocal microscope or multi-photon microscope, individually detecting fluorescent particles by detecting a light signal from the fluorescent particles present in the photodetection region while moving the location of the photodetection region in the sample solution, and counting the number of fluorescent particles detected during movement of the location of the photodetection region by counting the number of individually detected fluorescent particles. | 05-15-2014 |
20140134608 | SINGLE PARTICLE DETECTION DEVICE, SINGLE PARTICLE DETECTION METHOD, AND COMPUTER PROGRAM FOR SINGLE PARTICLE DETECTION, USING OPTICAL ANALYSIS - There is provided a single particle detection technique based on a scanning molecule counting method, enabling individual detection of a single particle using light measurement with a confocal or multiphoton microscope, and quantitative observation of conditions or characteristics of the particle. The inventive technique of detecting a single particle in a sample solution detects light containing substantially constant background light from a light detection region with moving the position of the light detection region of the microscope in a sample solution to generate time series light intensity data; and detects individually a light intensity reduction occurred when a single particle which does not emit light (or a particle whose emitting light intensity in a detected wavelength band is lower than the background light) enters in the light detection region in the time series light intensity data as a signal indicating the existence of each single particle. | 05-15-2014 |
20140162268 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - In the scanning molecule counting method detecting light of a light-emitting particle in a sample solution using a confocal or multiphoton microscope, there is provided an optical analysis technique enabling the scanning in a sample solution with moving a light detection region in a broader area or along a longer route while making the possibility of detecting the same light-emitting particle as different particles as low as possible and remaining the size or shape of the light detection region unchanged as far as possible. In the inventive optical analysis technique, there are performed detecting light from the light detection region and generating time series light intensity data during moving the light detection region along the second route whose position is moved along the first route, and thereby, the signal indicating light from each light-emitting particle in a predetermined route is individually detected using the time series light intensity data. | 06-12-2014 |
20140170760 | OPTICAL ANAYLSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided an optical analysis technique of detecting light of a light-emitting particle in a sample solution in the scanning molecule counting method using the light measurement with a confocal or multiphoton microscope, for suppressing the scattering in detected results of signals of light of light-emitting particles smaller and achieving the improvement of accuracy. The inventive technique comprises moving the position of a light detection region along a predetermined route for multiple circulation times by changing the optical path of the optical system; detecting light from the light detection region and generating time series light intensity data during the moving of the light detection region and detecting individually a signal indicating light from each light-emitting particle existing in the predetermined route using the time series light intensity data obtained in the circulating movements of the light detection region of multiple times. | 06-19-2014 |
20140175262 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided optical analysis techniques in the scanning molecule counting method using the light measurement with a confocal or multiphoton microscope in which the measuring unit time in the light measurement is set to an appropriate value in order to surely detect an approximately bell shape profile of the signal of a light-emitting particle and avoid excessive increase data volume of time series light intensity data. The inventive optical analysis technique of detecting light of a light-emitting particle in a sample solution generates time series light intensity data of light from a light detection region detected during moving the position of the light detection region of a microscope in the sample solution and detects in the data a signal indicating light from each light-emitting particle individually. The measuring unit time is determined based on the size and the moving speed of the light detection region. | 06-26-2014 |
20140231619 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE LIGHT-EMITTING PARTICLE DETECTION - There is provided a way of enabling the discrimination or identification of the kind of a light-emitting particle corresponding to each pulse form signal in the scanning molecule counting method using the optical measurement by the confocal or multiphoton microscope. In the inventive technique, the position of a light detection region in a sample solution periodically along a predetermined route is moved in measuring the light intensity from the light detection region; and a signal of light from a light-emitting particle is detected individually. Then, an index value indicating a translational diffusional characteristic of one light-emitting particle in a plane perpendicular to the moving direction of the light detection region is determined based upon intensity values of signals of light of the same light-emitting particle for identifying a light-emitting particle. | 08-21-2014 |
20140339444 | OPTICAL ANALYSIS DEVICE, OPTICAL ANALYSIS METHOD AND COMPUTER PROGRAM FOR OPTICAL ANALYSIS USING SINGLE PARTICLE DETECTION - In the optical analysis technique of detecting an existence of a single particle is a sample solution with a confocal microscope or a multiphoton microscope according to the scanning molecule counting method of the present invention, the position of a light detection region is moved in the sample solution; the light intensity from the light detection region is measured so that light intensity data will be generated; a first occurrence probability in assuming a first condition that no single particles exist in the light detection region and a second occurrence probability in assuming a second condition that a single particle exists in the light detection region for a time variation of light intensity value on the light intensity data are computed; and a signal indicating each single particle is detected based on those occurrence probabilities, and thereby enabling improvements in the sensitivity and/or S/N ratio. | 11-20-2014 |