Patent application number | Description | Published |
20090087879 | Method for the production of a lysate used for cell-free protein biosynthesis - The invention relates to a method for producing a lysate used for cell-free protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-free protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also disclosed are said lysate and the use thereof. | 04-02-2009 |
20100196952 | METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis of an expression product in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined expression product, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, without separating generated substances and without adding consumed synthesis substances within the defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products and/or reaction inhibitors are separated from the solution (and extracted), d) immediately before, after or at the same time as step c) consumed synthesis substances are supplemented, e) steps b), c) and d) are repeated at least once with the reaction solution of step d), and at the last execution of step b) steps c) and d) may be left out. | 08-05-2010 |
20120315669 | METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted). | 12-13-2012 |
20130115655 | METHOD FOR THE PRODUCTION OF A LYSATE USED FOR CELL-FREE PROTEIN BIOSYNTHESES - The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof. | 05-09-2013 |
20130261290 | METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted). | 10-03-2013 |
20140162313 | METHOD FOR PREPARATIVE IN VITRO PROTEIN BIOSYNTHESIS - The invention relates to a method for preparative in vitro protein synthesis in a cell-free transcription/translation system, comprising the following steps: a) in a reaction vessel, a reaction solution is prepared, comprising the following synthesis substances: components of the transcription/translation apparatus for a defined-protein, amino acids, and metabolic components supplying energy and being necessary for the synthesis of the defined protein, b) the synthesis is performed in the reaction vessel in a defined period of time, c) after expiration of the defined period of time, the reaction solution is subjected to a separation step, in which generated low-molecular metabolic products are separated from the solution (and extracted). | 06-12-2014 |
Patent application number | Description | Published |
20110174192 | SUGAR MICRO/NANOFOAMS - A method for producing rigid sugar micro-/nanofoams includes providing a solution with at least one sugar, a super- or near-critical fluid and a surfactant component as a (micro)emulsion. The (micro)emulsion is expanded so as to convert the super- or near-critical fluid from a state of liquid-like density to a state of gaseous density so as to thereby obtain a micro-/nanofoam. The micro-/nanofoam is then rigidified so as to obtain a rigid sugar micro-/nanofoam. | 07-21-2011 |
20120180741 | METHOD FOR THE IN SITU PRODUCTION OF FUEL/WATER MIXTURES IN COMBUSTION ENGINES - A process for operating an internal combustion engine or a nozzle includes producing a fuel mixture in-situ. The fuel mixture consists of a polar component A, a nonpolar fuel component B, an amphiphilic component C, and an auxiliary component D. The fuel mixture is produced in a high-pressure region of an injection system of an internal combustion engine or of a nozzle within 10 seconds of an injection operation. The fuel mixture is injected into the internal combustion engine or the nozzle. A pressure is in a range of from 100 to 4,000 bar. | 07-19-2012 |
20120238655 | METHOD FOR PRODUCING A POLYURETHANE FOAM AND POLYURETHANE FOAM OBTAINABLE THEREBY - A process for producing a polyurethane foam with bimodal cell size distribution, comprising the following steps:
| 09-20-2012 |
20120245243 | PROCESS FOR PRODUCING A POLYURETHANE FOAM BY MEANS OF SUPERCRITICAL OR NEAR-CRITICAL BLOWING AGENT - The present invention relates to a process for producing a polyurethane foam, where the blowing agent used is present in the supercritical or near-critical state. A reaction mixture is introduced into a closed mould, where the closed mould has been set up in such a way that its interior volume and/or the pressure prevailing in its interior can be altered after the introduction of the mixture by external influence. Through the selection of the surfactant it is possible to obtain microemulsions of the blowing agent in the polyol phase. The invention further relates to a nanocellular polyurethane foam obtainable by the process of the invention. | 09-27-2012 |
20130260133 | GENERATION OF NANODISPERSE INCLUSIONS IN A HIGH-VISCOSITY MATRIX - The invention relates to a process for generating a porous material having homogeneous, gas-containing inclusions in the micrometer and sub-micrometer range, and also to the material produced by such process. | 10-03-2013 |
20140011897 | REACTION MIXTURE IN THE FORM OF AN EMULSION AND PROCESS FOR PRODUCTION OF POLYURETHANE FOAMS FROM SUCH A REACTION MIXTURE - The present invention relates to a reaction mixture in emulsion form, suitable for conversion into polyurethanes, comprising a first phase and a second phase in the emulsion and further comprising the following components: A) polyols; B) blowing agent; C) surfactants; and D) isocyanates, wherein the isocyanate-reactive compounds A) are present in the first phase of the emulsion and the blowing agent B) is present in the second phase. The blowing agent B) is present in the near-critical or supercritical state and the isocyanate D) is present in the second phase in a proportion of ≧10% by weight of the total amount of isocyanate D) in the composition. The invention further relates to a method of producing polyurethane foams by providing such a reaction mixture, wherein a polymerization takes place at the freshly formed interface between the polyol phase and the blowing agent phase, to the use of such a reaction mixture for producing polyurethane foams and also to the polyurethane foams obtained. | 01-09-2014 |
20140045960 | POLYURETHANE FOAM AND METHOD FOR PRODUCING SAME - A polyurethane foam is obtainable from the reaction of a mixture comprising A) an isocyanate-reactive compound; B) a blowing agent selected from the group comprising linear, branched or cyclic C | 02-13-2014 |
20140107239 | METHOD FOR PRODUCING A FOAMED MATERIAL,COMPOSITION IN THE FORM OF AN EMULSION USED IN SAID METHOD, AND FOAMED MATERIAL THAT CAN BE OBTAINED FROM SAID METHOD - The present invention relates to a process for producing a foamed material, wherein a composition in the form of emulsion with a matrix-forming component, a surfactant component and a near-critical or supercritical blowing agent component is submitted to a lowering of pressure. The blowing agent component further comprises a hydrophobic co-component, which is soluble in supercritical CO | 04-17-2014 |