Patent application number | Description | Published |
20120014976 | COMPOSITIONS AND METHODS FOR ENHANCING IMMUNE RESPONSES TO VACCINES - The disclosure provides adjuvants, immunogenic compositions, and methods useful for vaccination and immune response. In particular, the disclosure provides a class of adjuvants comprising cationic lipid:co-lipid mixtures and methods for delivering formulated compositions. | 01-19-2012 |
20120141577 | LINEAR EXPRESSION CASSETTE VACCINES - The disclosure relates to a linear expression cassette (LEC) as a nucleic acid based vector for producing a gene product of interest. Methods of preparing a disclosed LEC, as well as methods for its use to express a gene product in a subject, are also described. An LEC may be used in an animal or human subject to produce a therapeutic and/or immune response in the subject, such as an immunized state. | 06-07-2012 |
20130045222 | COMPOSITIONS AND METHODS FOR ENHANCING IMMUNE RESPONSES TO VACCINES - The disclosure provides adjuvants, immunogenic compositions, and methods useful for vaccination and immune response. In particular, the disclosure provides a class of adjuvants comprising cationic lipid:co-lipid mixtures and methods for delivering formulated compositions. | 02-21-2013 |
Patent application number | Description | Published |
20130071403 | SYNERGISTIC ANTI-TUMOR EFFICACY USING ALLOANTIGEN COMBINATION IMMUNOTHERAPY - The present disclosure provides combinations of immunotherapeutics and methods for treating medical conditions that are characterized by the lack of an effective immune response, for example as would result following a down-regulation of MHC class I, such as in cancer. The immunotherapeutic compositions of the invention, which can be used to treat the medical conditions, include one or more immunostimulatory antibodies or molecules having specificity for CTLA-4, PD-1, PD-L1, PD-L2, CD40, OX40, CD137, GITR, ILT2, or ILT3, or ligands for these molecules (e.g., an isolated fully-human monoclonal antibody) in association with one or more alloantigens, such as, vector(s) capable of expressing protein(s) or peptide(s) that stimulate T-cell immunity against tissues or cells, formulated in a pharmaceutically acceptable carrier. The proteins or peptides may comprise class I major histocompatibility complex (MHC) antigens, β2-microglobulins, or cytokines. The MHC antigen may be foreign to the subject. The MHC antigen may be HLA-B7. | 03-21-2013 |
20130202630 | COMPOSITIONS AND METHODS FOR ENHANCING IMMUNE RESPONSES TO VACCINES - The disclosure provides adjuvants, immunogenic compositions, and methods useful for vaccination and immune response. In particular, the disclosure provides a class of adjuvants comprising cationic lipid:co-lipid mixtures and methods for delivering formulated compositions. | 08-08-2013 |
20130273078 | SYNERGISTIC ANTI-TUMOR EFFICACY USING ALLOANTIGEN COMBINATION IMMUNOTHERAPY - The present disclosure provides combinations of immunotherapeutics and methods for treating medical conditions that are characterized by the lack of an effective immune response, for example as would result following a down-regulation of MHC class I, such as in cancer. The immunotherapeutic compositions of the invention, which can be used to treat the medical conditions, include one or more immunostimulatory antibodies or molecules having specificity for CTLA-4, PD-1, PD-L1, PD-L2, CD40, OX40, CD137, GITR, ILT2, or ILT3, or ligands for these molecules (e.g., an isolated fully-human monoclonal antibody) in association with one or more alloantigens, such as, vector(s) capable of expressing protein(s) or peptide(s) that stimulate T-cell immunity against tissues or cells, formulated in a pharmaceutically acceptable carrier. The proteins or peptides may comprise class I major histocompatibility complex (MHC) antigens, β2-microglobulins, or cytokines. The MHC antigen may be foreign to the subject. The MHC antigen may be HLA-B7. | 10-17-2013 |
20130280265 | SYNERGISTIC ANTI-TUMOR EFFICACY USING ALLOANTIGEN COMBINATION IMMUNOTHERAPY - The present disclosure provides combinations of immunotherapeutics and methods for treating medical conditions that are characterized by the lack of an effective immune response, for example as would result following a down-regulation of MHC class I, such as in cancer. The immunotherapeutic compositions of the invention, which can be used to treat the medical conditions, include one or more immunostimulatory antibodies or molecules having specificity for CTLA-4, PD-1, PD-L1, PD-L2, CD40, OX40, CD137, GITR, ILT2, or ILT3, or ligands for these molecules (e.g., an isolated fully-human monoclonal antibody) in association with one or more alloantigens, such as, vector(s) capable of expressing protein(s) or peptide(s) that stimulate T-cell immunity against tissues or cells, formulated in a pharmaceutically acceptable carrier. The proteins or peptides may comprise class I major histocompatibility complex (MHC) antigens, β2-microglobulins, or cytokines. The MHC antigen may be foreign to the subject. The MHC antigen may be HLA-B7. | 10-24-2013 |
Patent application number | Description | Published |
20080284056 | MULTILAYER EXTRUDED SEAL AND METHOD FOR MANUFACTURING SUCH SEAL - An extruded multilayer seal member having an expanded temperature range and an improved compression set resistance wherein the seal member comprises a first fluoroelastomer as the inner layer of the multilayer seal member and a second elastomer as the outer layer of the multilayer seal member; and a method for manufacturing the multilayer seal member which comprises extruding a multilayer tubular structure, crosslinking the extruded multilayer tubular structure on a mandrel, cutting the multilayer tubular structure to provide a plurality of uniform seal members and recovering the seal members, are described. | 11-20-2008 |
20100031912 | ENGINE AIR INTAKE MANIFOLD HAVING A SHELL - Apparatus includes a motor-vehicle, internal-combustion-engine air intake manifold having an intake-manifold first shell attachable or monolithically joined to an intake-manifold second shell to surround an internal manifold volume. The first shell has a wall with at least a portion having undulating concave and convex regions as defined exterior to the internal manifold volume of the attached or monolithically-joined first shell and second shell. | 02-11-2010 |
20100031913 | MODULAR INTAKE MANIFOLD - Disclosed herein is an intake manifold assembly that comprises a first outer covering, a second outer covering connected to the first outer covering that together define a chamber having an intake port, and a flow channel insert. The second outer covering has an outlet port positioned within its interior surface and the flow channel insert is nestingly connected to the outlet port with the rest of the flow channel insert received within the chamber. The flow channel insert being a single piece that defines a channel having a first end and a second end. | 02-11-2010 |
20100059009 | ASSEMBLY AND METHOD FOR CONTROLLING AN AIR INTAKE RUNNER - An assembly for controlling an air intake runner of an air intake manifold. The assembly includes a cartridge including a plurality of compartments. Each of the compartments has a plurality of that are joined to define a central opening and an outer perimeter. Adjacent compartments are spaced apart to define a groove between each of the compartments. The assembly also includes bushing carriers that are configured to be snap-fit into one of the grooves of the cartridge. The assembly further includes bushings configured to rotatably fit within the central openings of the bushing carriers. Each of the bushings has an outer rim and an open center. Flaps including a slot and having a shape configured to substantially adjustably seal the central opening are also included and a shaft is included that extends through the slots of the flaps and the open centers of the bushings. | 03-11-2010 |
20100059700 | DUAL OUTPUT FLOW CONTROL ACTUATOR - Disclosed herein is an actuator for regulating the flow of fluids in first and second flow paths of an internal combustion engine. The actuator includes a motor having a rotatable drive shaft, a gear assembly driven by the drive shaft of the motor, and first and second output shafts rotated in opposite directions by the gear assembly. The first and second output shafts are operatively linked to valves in the first and second flow paths, respectively, to regulate the flow of fluids in the first and second flow paths. | 03-11-2010 |
20110303178 | ASSEMBLY AND METHOD FOR CONTROLLING AN AIR INTAKE RUNNER - A method of modifying an air intake manifold to control air intake runners. The method includes the steps of providing a cartridge including a plurality of compartments, each spaced apart to define a groove therebetween, snap-fitting a bushing carrier having a rotatable bushing into each of the grooves, positioning a flap having a slot over each of the compartments, inserting a shaft through each slot and each bushing thereby rotatably retaining each of the flaps within one of the plurality of compartments, positioning the cartridge over the air intake manifold so that each of the plurality of compartments is substantially axially aligned with one of the air intake runners, and removably connecting the cartridge with the air intake manifold. | 12-15-2011 |
20120210970 | ASSEMBLY AND METHOD FOR CONTROLLING AN AIR INTAKE RUNNER - A subassembly for a valve device for controlling air flow in an intake manifold. The subassembly includes a cartridge including a plurality of compartments having partially open opposing sidewalls, wherein adjacent partially open side walls of adjacent compartments are spaced apart a distance to define a groove therebetween, a plurality of partitions each having a through-hole therein and being disposed in the grooves defined between adjacent partially open side walls of the adjacent compartments. The partitions complete the formation of the opposing side walls of the adjacent compartments and each compartment is alignable with an air intake runner of an intake manifold. | 08-23-2012 |
Patent application number | Description | Published |
20110170111 | OPTICAL COHERENCE TOMOGRAPHY (OCT) APPARATUS, METHODS, AND APPLICATIONS - A free-space Michelson Interferometer-based Dual Detection Frequency Domain-Optical Coherency Tomography (DD-FD-OCT) apparatus includes a non-polarizing beam splitter that can be used to misalign sample and reference beam paths to provide a stable π/2 phase shift between simultaneously detected interfering sample and reference beams to eliminate the mirror image created by Fourier transformation during image reconstruction. A hybrid fiber system Mach Zehnder Interferometer- and free-space Michelson Interferometer-based Dual Detection Frequency Domain-Doppler Optical Coherency Tomography (DD-FD-DOCT) apparatus provides higher power efficiency and thus better sensitivity compared to the free-space DD-FD-OCT. Both DD-FD-OCT systems enable functional imaging with the contrasts of Doppler and that of polarization, in addition to full range images simultaneously. | 07-14-2011 |
20120243001 | OPTICAL TESTING APPARATUS AND METHODS - An apparatus and associated method for testing a non-symmetric (e.g., phi-polynomial) surface. The apparatus uses several simple (singlet) optical elements (e.g., an Offner null configuration) and a tilted optic under test in combination with an active optical element (e.g., actuated, deformable membrane mirror, optical phase modulator, etc.) that together form a null or quasi-null that allows for conventional null-based interferometry. This solution solves the problem of exceeding the dynamic range of a conventional interferometer when trying to test non-symmetric optical surfaces. | 09-27-2012 |
20120243114 | NONSYMMETRIC OPTICAL SYSTEM AND DESIGN METHOD FOR NONSYMMETRIC OPTICAL SYSTEM - A new family of truly nonsymmetric optical systems that exploit a new fabrication degree of freedom enabled by the introduction of slow-servos to diamond machining; surfaces whose departure from a sphere varies both radially and azimuthally in the aperture, and associated design method. | 09-27-2012 |
20130029286 | DEVICES AND METHODS FOR CONFORMING PHOTODYNAMIC THERAPY TO SPECIFIC ANATOMIC LOCATIONS - A device for conforming photodynamic therapy to a specific anatomic location (e.g., in the oral cavity) conforms the radiation to the target tissue surface and avoids delivering light to the rest of the oral cavity. Embodiments can include a body of oral impression material molded to conform to the anatomic surface, a light pipe, a freeform reflector formed on a surface of the optical body, or a light guide having a textured surface to direct light to an opposing output window. The light guide can be made of flexible plastic to conform the output window to the lesion. | 01-31-2013 |
20130044313 | Broad Band Czerny-Turner Spectrometer, Methods, and Applications - A low-cost optics, broadband, astigmatism-corrected practical spectrometer. An off-the-shelf cylindrical lens is used to remove astigmatism over the full bandwidth, providing better than 0.1 nm spectral resolution and more than 50% through-put over a bandwidth of 400 nm centered at 800 nm. The spectrometer includes a first spherical mirror disposed along an optical path in an off-axis (tilted) orientation; a diffraction grating disposed along the optical axis in a location optically downstream from the first mirror; a second spherical mirror disposed along the optical path in an off-axis orientation in a location optically down-stream from the diffraction grating; a cylindrical optic disposed in the optical path; and a detector disposed in the optical path in a location optically downstream from the second spherical mirror. | 02-21-2013 |
20150216407 | MEASUREMENT OF THE LIPID AND AQUEOUS LAYERS OF A TEAR FILM - Systems and methods for determining thickness of lipid and aqueous layers of a tear film in which a spectrum array is generated from optical coherence tomography and input into a statistical estimator, which determines the thickness of the lipid and/or aqueous layers at a nanometer resolution based on the inputted spectrum and other information, such as information about a laser intensity noise, Poisson noise, and dark noise associated with the OCT. | 08-06-2015 |
20160091723 | OPTICAL DISPLAY APPARATUS, METHOD, AND APPLICATIONS - A see-through optical display apparatus includes an image generating component, a tilted primary mirror having a non-flat, freeform, front optical surface, and a tilted secondary mirror having a non-flat, freeform, front optical surface, wherein the apparatus has an external pupil. A method for designing/making a see-through optical display apparatus for displaying an image generated by or on an image generating component of the apparatus. | 03-31-2016 |
Patent application number | Description | Published |
20110215045 | HIGH FIDELITY THROUGH HOLE FILM, AND ASSOCIATED METHOD - A membrane is provided, comprising a first membrane layer having a first side and a second side. The first membrane layer defines a plurality of holes extending along a first axis between the first side and the second side. Each hole is defined by the first membrane layer as a complex three-dimensional shape, and each hole has a diameter of less than about 10 micrometers. The membrane is fabricated by dispersing a liquid polymeric material onto a patterned master template, hardening the polymeric material, and removing it from the master template. The membrane includes through holes which correspond to the structures of the patterned master template in size, cross-sectional and three dimensional shape, orientation, and the like. | 09-08-2011 |
20110300293 | SYSTEM AND METHOD FOR PRODUCING PARTICLES AND PATTERNED FILMS - A system including a mold having a fluoropolymer wherein the mold defines a plurality of cavities having a predetermined shape and a cross-sectional dimension less than about 100 micrometers; a roller; a surface in cooperation with the roller to form a nip point configured to receive the mold, wherein the nip point is further configured to receive a substantially liquid composition and accelerate entry of the substantially liquid composition into the cavity. A method of forming particles including applying a substantially liquid composition to a mold, wherein the mold comprises a fluoropolymer and defines a plurality of cavities each having a broadest cross-sectional dimension of less than about 100 micrometers; nipping the mold between a roller and a surface such that the substantially liquid composition enters the cavities of the mold; and hardening the substantially liquid composition in the cavities of the mold to form a particle within each cavity, wherein the particle has a size and shape that substantially mimics the size and shape of the cavity of the mold. | 12-08-2011 |
20130084630 | QUANTITATIVE MICROFLUIDIC DEVICES - Described herein are disposable paper-based assay devices for detection and quantitation of analytes in liquid clinical samples, e.g., blood or urine. The devices may be particularly suitable for use in regions of the world where health care infrastructure is absent. The test devices are versatile in that they can be adapted to detect a variety of analytes. The devices are also easy to use and interpret. Typically, all that is needed to conduct an assay is to apply a drop of sample to the indicated location on the device. The devices are typically colorimetric and readable with the naked eye. | 04-04-2013 |
20140027948 | SYSTEM AND METHOD FOR PRODUCING PARTICLES AND PATTERNED FILMS - A system including a mold having a fluoropolymer wherein the mold defines a plurality of cavities having a predetermined shape and a cross-sectional dimension less than about 100 micrometers; a roller; a surface in cooperation with the roller to form a nip point configured to receive the mold, wherein the nip point is further configured to receive a substantially liquid composition and accelerate entry of the substantially liquid composition into the cavity. A method of forming particles including applying a substantially liquid composition to a mold, wherein the mold comprises a fluoropolymer and defines a plurality of cavities each having a broadest cross-sectional dimension of less than about 100 micrometers; nipping the mold between a roller and a surface such that the substantially liquid composition enters the cavities of the mold; and hardening the substantially liquid composition in the cavities of the mold to form a particle within each cavity, wherein the particle has a size and shape that substantially mimics the size and shape of the cavity of the mold. | 01-30-2014 |
20140295415 | LOW COST, DISPOSABLE MOLECULAR DIAGNOSTIC DEVICES - The invention provides molecular diagnostic test devices and methods for using such diagnostic test devices to detect analytes of biological significance in a patient. The diagnostic test devices are particularly useful for detecting a polynucleotide analyte in a sample obtained from a patient. Further, the diagnostic test devices are inexpensive, disposable, easy to use, and are useful at the point of care. | 10-02-2014 |
Patent application number | Description | Published |
20160136889 | METHODS OF PRODUCING THREE-DIMENSIONAL OBJECTS FROM MATERIALS HAVING MULTIPLE MECHANISMS OF HARDENING - A method of forming a three-dimensional object is carried out by: (a) providing a carrier and an optically transparent member having a build surface, the carrier and the build surface defining a build region therebetween; (b) filling the build region with a polymerizable liquid, the polymerizable liquid including a mixture of (i) a light polymerizable liquid first component, and (ii) a second solidifiable component that is different from the first component; (c) irradiating the build region with light through the optically transparent member to form a solid polymer scaffold from the first component and also advancing the carrier away from the build surface to form a three-dimensional intermediate having the same shape as, or a shape to be imparted to, the three-dimensional object, and containing the second solidifiable component carried in the scaffold in unsolidified and/or uncured form; and (d) concurrently with or subsequent to the irradiating step, solidifying and/or curing the second solidifiable component in the three-dimensional intermediate to form the three-dimensional object. | 05-19-2016 |
20160137838 | METHODS OF PRODUCING POLYURETHANE THREE-DIMENSIONAL OBJECTS FROM MATERIALS HAVING MULTIPLE MECHANISMS OF HARDENING - A method of forming a three-dimensional object of polyurethane, polyurea, or copolymer thereof is carried out by: (a) providing a carrier and an optically transparent member having a build surface, the carrier and the build surface defining a build region therebetween; (b) filling the build region with a polymerizable liquid, the polymerizable liquid including at least one of: (i) a blocked or reactive blocked prepolymer, (ii) a blocked or reactive blocked diisocyanate, or (iii) a blocked or reactive blocked diisocyanate chain extender; (c) irradiating the build region with light through the optically transparent member to form a solid blocked polymer scaffold and advancing the carrier away from the build surface to form a three-dimensional intermediate having the same shape as, or a shape to be imparted to, the three-dimensional object, with the intermediate containing the chain extender; and then (d) heating or microwave irradiating the three-dimensional intermediate sufficiently to form from the three-dimensional intermediate the three-dimensional object of polyurethane, polyurea, or copolymer thereof. | 05-19-2016 |
20160137839 | POLYURETHANE RESINS HAVING MULTIPLE MECHANISMS OF HARDENING FOR USE IN PRODUCING THREE-DIMENSIONAL OBJECTS - A polymerizable liquid, or resin, useful for the production by additive manufacturing of a three-dimensional object of polyurethane, polyurea, or a copolymer thereof, is described. The resin includes at least one of (i) a blocked or reactive blocked prepolymer, (ii) a blocked or reactive blocked diisocyanate, or (iii) a blocked or reactive blocked diisocyanate chain extender. | 05-19-2016 |
Patent application number | Description | Published |
20120189728 | METHODS AND MATERIALS FOR FABRICATING LAMINATE NANOMOLDS AND NANOPARTICLES THEREFROM - A laminate nanomold includes a layer of perfluoropolyether defining a cavity that has a predetermined shape and a support layer coupled with the layer of perfluoropolyether. The laminate can also include a tie-layer coupling the layer of perfluoropolyether with the support layer. The tie-layer can also include a photocurable component and a thermal curable component. The cavity can have a broadest dimension of less than 500 nanometers. | 07-26-2012 |
20120256354 | METHODS AND MATERIALS FOR FABRICATING MICROFLUIDIC DEVICES - Materials and Methods are provided for fabricating microfluidic devices. The materials include low surface energy fluoropolymer compositions having multiple cure functional groups. The materials can include multiple photocurable and/or thermal-curable functional groups such that laminate devices can be fabricated. The materials also substantially do not swell in the presence of hydrocarbon solvents. | 10-11-2012 |
20130011618 | PHOTOCURABLE PERFLUOROPOLYETHERS FOR USE AS NOVEL MATERIALS IN MICROFLUIDIC DEVICES - A functionalized photocurable perfluoropolyether is used as a material for fabricating a solvent-resistant microfluidic device. Such solvent-resistant microfluidic devices can be used to control the flow of small amounts of a fluid, such as an organic solvent, and to perform microscale chemical reactions that are not amenable to other polymer-based microfluidic devices. | 01-10-2013 |
20130202729 | Methods and Materials for Fabricating Laminate Nanomolds and Nanoparticles Therefrom - A laminate nanomold includes a layer of perfluoropolyether defining a cavity that has a predetermined shape and a support layer coupled with the layer of perfluoropolyether. The laminate can also include a tie-layer coupling the layer of perfluoropolyether with the support layer. The tie-layer can also include a photocurable component and a thermal curable component. The cavity can have a broadest dimension of less than 500 nanometers. | 08-08-2013 |
20130228950 | METHODS AND MATERIALS FOR FABRICATING MICROFLUIDIC DEVICES - Materials and methods are provided for fabricating microfluidic devices. The materials include low surface energy fluoropolymer compositions having multiple cure functional groups. The materials can include multiple photocurable and/or thermal-curable functional groups such that laminate devices can be fabricated. The materials also substantially do not swell in the presence of hydrocarbon solvents. | 09-05-2013 |
20140131915 | Methods and Materials for Fabricating Laminate Nanomolds and Nanoparticles Therefrom - A laminate nanomold includes a layer of perfluoropolyether defining a cavity that has a predetermined shape and a support layer coupled with the layer of perfluoropolyether. The laminate can also include a tie-layer coupling the layer of perfluoropolyether with the support layer. The tie-layer can also include a photocurable component and a thermal curable component. The cavity can have a broadest dimension of less than 500 nanometers. | 05-15-2014 |
20150101743 | Methods and Materials for Fabricating Laminate Nanomolds and Nanoparticles Therefrom - A laminate nanomold includes a layer of perfluoropolyether defining a cavity that has a predetermined shape and a support layer coupled with the layer of perfluoropolyether. The laminate can also include a tie-layer coupling the layer of perfluoropolyether with the support layer. The tie-layer can also include a photocurable component and a thermal curable component. The cavity can have a broadest dimension of less than 500 nanometers. | 04-16-2015 |
20160038418 | NANOPARTICLE FABRICATION METHODS, SYSTEMS, AND MATERIALS - Nano-particles are molded in nano-scale molds fabricated from non-wetting, low surface energy polymeric materials. The nano-particles can include pharmaceutical compositions, taggants, contrast agents, biologic drugs, drug compositions, organic materials, and the like. The molds can be virtually any shape and less than 10 micron in cross-sectional diameter. | 02-11-2016 |
Patent application number | Description | Published |
20090092628 | Conserved-element vaccines and methods for designing conserved-element vaccines - Embodiments of the present invention include conserved-element vaccines and methods for designing and producing conserved-element vaccines. A conserved-element vaccine (“CEVac”) is a recombinant and/or synthetic vaccine that incorporates only highly conserved epitopes from an observed set of pathogen variants. The conserved epitopes are identified computationally by aligning biopolymer sequences, such as concatenated polypeptide sequences that together represent a pathogen proteome, corresponding to an observed set of pathogen variants, and computationally selecting conserved subsequences according to a number of subsequence-selection criteria. These subsequence-selection criteria may include a minimum conserved-subsequence length, a threshold frequency of occurrence of a particular monomer at each conserved, single-monomer position within a conserved subsequence, a threshold combined occurrence for a set of allowable variant monomers at a particular conserved, variable position within a conserved subsequence, and a maximum number of variable positions within a subsequence. A set of conserved subsequences identified according to the subsequence-selection criteria are then filtered to remove subsequences identical to, or too similar to, naturally-occurring host subsequences, and are then assembled into expression vectors for incorporation into microbial hosts for biosynthesis of a recombinant CEVac or assembled into one or more synthetic constructs for a synthetic CEVac. | 04-09-2009 |
20110269937 | Conserved-Element Vaccines and Methods for Designing Conserved-Element Vaccines - Embodiments of the present invention include conserved-element vaccines and methods for designing and producing conserved-element vaccines. A conserved-element vaccine (“CEVac”) is a recombinant and/or synthetic vaccine that incorporates only highly conserved epitopes from an observed set of pathogen variants. The conserved epitopes are identified computationally by aligning biopolymer sequences, such as concatenated polypeptide sequences that together represent a pathogen proteome, corresponding to an observed set of pathogen variants, and computationally selecting conserved subsequences according to a number of subsequence-selection criteria. These subsequence-selection criteria may include a minimum conserved-subsequence length, a threshold frequency of occurrence of a particular monomer at each conserved, single-monomer position within a conserved subsequence, a threshold combined occurrence for a set of allowable variant monomers at a particular conserved, variable position within a conserved subsequence, and a maximum number of variable positions within a subsequence. A set of conserved subsequences identified according to the subsequence-selection criteria are then filtered to remove subsequences identical to, or too similar to, naturally-occurring host subsequences, and are then assembled into expression vectors for incorporation into microbial hosts for biosynthesis of a recombinant CEVac or assembled into one or more synthetic constructs for a synthetic CEVac. | 11-03-2011 |