Patent application number | Description | Published |
20110039732 | cDNA Synthesis Using Non-Random Primers - The present invention provides methods for selectively amplifying a target population of nucleic acid molecules in a population of RNA template molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides a first population of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:1-749 and a second population of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:750-1498. The first population of oligonucleotides can be used, for example, to prime the synthesis of first strand cDNA molecules complementary to mRNA molecules isolated from mammalian cells without priming the synthesis of cDNA molecules complementary to ribosomal RNA molecules. The second population of oligonucleotides can be used, for example, to prime the second strand synthesis of primer extension products (first strand cDNA) complementary to mRNA molecules isolated from mammalian cells without priming the second strand synthesis of primer extension products synthesized from ribosomal RNA molecules. | 02-17-2011 |
20110319290 | Methods and Compositions for Multiplex Sequencing - Adapters are joined to target polynucleotides to create adapter-tagged polynucleotides. Adapter-tagged polynucleotides are sequenced simultaneously and sample sources are identified on the basis of barcode sequences. | 12-29-2011 |
20120015821 | Methods of Generating Gene Specific Libraries - The invention provides compositions and methods for generating a target enriched, sequencing ready library for resequencing at least one target region of interest from a nucleic acid containing sample. | 01-19-2012 |
20130252823 | cDNA SYNTHESIS USING NON-RANDOM PRIMERS - The present invention provides methods for selectively amplifying a target population of nucleic acid molecules in a population of RNA template molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The invention also provides a method of generating a population of oligonucleotide primers for transcriptome profiling of total RNA from a subject of interest. | 09-26-2013 |
Patent application number | Description | Published |
20090030186 | PICHIA METHANOLICA SECRETORY SIGNAL | 01-29-2009 |
20090123912 | Methods for quantitating small RNA molecules - In one aspect, the present invention provides methods for amplifying a microRNA molecule to produce DNA molecules. The methods each include the steps of: (a) using primer extension to make a DNA molecule that is complementary to a target microRNA molecule; and (b) using a universal forward primer and a reverse primer to amplify the DNA molecule to produce amplified DNA molecules. In some embodiments of the method, at least one of the forward primer and the reverse primer comprise at least one locked nucleic acid molecule. | 05-14-2009 |
20100029511 | CDNA SYNTHESIS USING NON-RANDOM PRIMERS - The present invention provides methods for selectively amplifying a target population of nucleic acid molecules in a population of RNA template molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The invention also provides a method of generating a population of oligonucleotide primers for transcriptome profiling of total RNA from a subject of interest. | 02-04-2010 |
20110003301 | METHODS FOR DETECTING GENETIC VARIATIONS IN DNA SAMPLES - The invention provides methods, compositions and kits for detecting genetic variation in a DNA sample at one or more polymorphic loci of interest. In some embodiments, the invention provides methods, compositions, and kits for determining the nucleotide present at a single nucleotide variant position of interest in a test sample. | 01-06-2011 |
20110294701 | Nucleic acid amplification using non-random primers - The present invention provides methods for selectively amplifying a target population of nucleic acid molecules (e.g., all mRNA molecules expressed in a cell type except for the most highly expressed mRNA species). The present invention also provides populations of oligonucleotides including the nucleic acid sequences set forth in SEQ ID NOS:1-933. These oligonucleotides can be used, for example, to prime the synthesis of cDNA molecules complementary to mRNA molecules isolated from mammalian blood without priming the synthesis of cDNA molecules complementary to globin mRNA, or ribosomal RNA molecules. | 12-01-2011 |
20120009580 | METHODS FOR QUANTITATING SMALL RNA MOLECULES - In one aspect, the present invention provides methods for amplifying a microRNA molecule to produce DNA molecules. The methods each include the steps of: (a) using primer extension to make a DNA molecule that is complementary to a target microRNA molecule; and (b) using a universal forward primer and a reverse primer to amplify the DNA molecule to produce amplified DNA molecules. In some embodiments of the method, at least one of the forward primer and the reverse primer comprise at least one locked nucleic acid molecule. | 01-12-2012 |
20140274731 | METHODS FOR TARGETED GENOMIC ANALYSIS - The invention provides a method for genetic analysis in individuals that reveals both the genetic sequences and chromosomal copy number of targeted and specific genomic loci in a single assay. The present invention further provide methods for the sensitive and specific detection of target gene sequences and gene expression profiles. | 09-18-2014 |