Patent application number | Description | Published |
20090175865 | CYSTEINE ENGINEERED ANTIBODIES AND CONJUGATES - Antibodies are engineered by replacing one or more amino acids of a parent antibody with non cross-linked, highly reactive cysteine amino acids. Antibody fragments may also be engineered with one or more cysteine amino acids to form cysteine engineered antibody fragments (ThioFab). Methods of design, preparation, screening, and selection of the cysteine engineered antibodies are provided. Cysteine engineered antibodies (Ab), optionally with an albumin-binding peptide (ABP) sequence, are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered antibody-drug conjugates having Formula I: | 07-09-2009 |
20100003766 | CYSTEINE ENGINEERED ANTIBODIES AND CONJUGATES - Antibodies are engineered by replacing one or more amino acids of a parent antibody with non cross-linked, highly reactive cysteine amino acids. Antibody fragments may also be engineered with one or more cysteine amino acids to form cysteine engineered antibody fragments (ThioFab). Methods of design, preparation, screening, and selection of the cysteine engineered antibodies are provided. Cysteine engineered antibodies (Ab), optionally with an albumin-binding peptide (ABP) sequence, are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered antibody-drug conjugates having Formula I: | 01-07-2010 |
20110137017 | CYSTEINE ENGINEERED ANTIBODIES AND CONJUGATES - Antibodies are engineered by replacing one or more amino acids of a parent antibody with non cross-linked, highly reactive cysteine amino acids. Antibody fragments may also be engineered with one or more cysteine amino acids to form cysteine engineered antibody fragments (ThioFab). Methods of design, preparation, screening, and selection of the cysteine engineered antibodies are provided. Cysteine engineered antibodies (Ab), optionally with an albumin-binding peptide (ABP) sequence, are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered antibody-drug conjugates having Formula I: | 06-09-2011 |
20150017188 | CYSTEINE ENGINEERED ANTIBODIES AND CONJUGATES - Antibodies are engineered by replacing one or more amino acids of a parent antibody with non cross-linked, highly reactive cysteine amino acids. Antibody fragments may also be engineered with one or more cysteine amino acids to form cysteine engineered antibody fragments (ThioFab). Methods of design, preparation, screening, and selection of the cysteine engineered antibodies are provided. Cysteine engineered antibodies (Ab), optionally with an albumin-binding peptide (ABP) sequence, are conjugated with one or more drug moieties (D) through a linker (L) to form cysteine engineered antibody-drug conjugates having Formula I: | 01-15-2015 |
Patent application number | Description | Published |
20100159510 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 06-24-2010 |
20110111442 | Intein-modified enzymes, their production and industrial applications - A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. | 05-12-2011 |
20110138502 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 06-09-2011 |
20120258503 | CONSOLIDATED PRETREATMENT AND HYDROLYSIS OF PLANT BIOMASS EXPRESSING CELL WALL DEGRADING ENZYMES - Methods for consolidated pretreatment and hydrolysis of genetically engineered plants expressing cell wall degrading enzymes are provided. Expression cassettes and vectors for making transgenic plants are described. Plants engineered to express one or more cell wall degrading enzymes using expression cassettes and vectors of the invention are also provided. | 10-11-2012 |
20120291160 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AN RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 11-15-2012 |
20130007919 | INTEIN-MODIFIED ENZYMES, THEIR PRODUCTION AND INDUSTRIAL APPLICATIONS - Intein modified proteins, isolated nucleic acids encoding intein modified proteins, fragments of intein modified proteins, isolated nucleic acids encoding fragments of intein modified proteins, transgenic plants containing any of the foregoing, and antibodies recognizing epitopes on intein modified proteins are provided | 01-03-2013 |
20130036517 | INTEIN-MODIFIED ENZYMES, THEIR PRODUCTION AND INDUSTRIAL APPLICATIONS - A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. | 02-07-2013 |
20130071884 | PLANTS EXPRESSING CELL WALL DEGRADING ENZYMES AND EXPRESSION VECTORS - Vectors for expression of proteins in plants are described. The proteins may be enzymes and the enzymes can be but are not limited to cell wall degrading enzymes. A number of plants designed to express specific cell wall degrading enzymes are provided. The plants may have industrial and/or agricultural applications. Methods and materials for making the expression vectors and for making the plants are provided. Processes for which the plants could be used in industrial and agricultural applications are also provided. | 03-21-2013 |
20130203125 | CELLULOSIC PROCESSING TRAIT DEVELOPMENT USING A THERMOREGULATED, INTEIN-MODIFIED XYLANASE - In planta consolidated bioprocessing has the advantages of decreasing biomass pretreatment costs, utilizing excess plant protein production capacity for enzyme production, and decreasing mass transfer resistance of enzyme diffusion to its substrate. However, in planta expression of cell wall degrading (CWD) enzymes often leads to detrimental plant phenotypes that impact crop yield. To provide in planta CWD enzyme activity without any adverse phenotype, a thermostable xylanase, XynB (EC 3.2.1.8), was engineered with a thermoregulated intein, Tth-HB27 DnaE-1 (Tth intein), that controls its hydrolytic activity through conditional intein splicing. Maize plants expressing the heat inducible Tth intein-modified XynB developed normally, yet possessed enhanced post harvest glucose production from dried corn stover. Expression of CWD enzymes as dormant, intein-modified proteins that can be activated by heat treatment after harvest provides the basis for developing a novel cellulosic processing trait in plants. | 08-08-2013 |
20130247251 | INTEIN-MODIFIED ENZYMES, THEIR PRODUCTION AND INDUSTRIAL APPLICATION - A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided. | 09-19-2013 |
20130269061 | METHODS AND COMPOSITIONS FOR PROCESSING BIOMASS WITH ELEVATED LEVELS OF STARCH - Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described. | 10-10-2013 |
20130318655 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 11-28-2013 |
20130318656 | PLANTS WITH ALTERED LEVELS OF VEGETATIVE STARCH - Vectors for altering the expression of one or more starch regulation enzymes are provided. Methods of transformation of plant tissues to express elements altering expression of one or more starch regulation enzymes, and resulting transgenic plants are provided. Methods of using the transgenic plants are provided. | 11-28-2013 |
20140314909 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 10-23-2014 |
20150225735 | STRONG CONSTITUTIVE PROMOTERS FOR HETEROLOGOUS EXPRESSION OF PROTEINS IN PLANTS - Nucleic acid promoters isolated from | 08-13-2015 |
20150232827 | INTEIN-MODIFIED PROTEASES, THEIR PRODUCTION AND INDUSTRIAL APPLICATIONS - Methods for producing intein-modified proteases are provided. Expression cassettes and vectors for using to genetically engineer hosts are described. Hosts genetically engineered to express one or more intein-modified proteases using expression cassettes and vectors of the invention are also provided. Methods to produce a protease and regulate its activity are described. | 08-20-2015 |
20150267206 | MULTIPROTEIN EXPRESSION CASSETTES - Methods for expressing multiple proteins by constructing transformation vectors that include multiprotein expression cassettes and transforming hosts with vectors and by engineering hosts expressing multiprotein units are provided. Multiprotein units that include multiple proteins fused to modified inteins capable of effecting splicing of the multiprotein units are described. Expression cassettes that include nucleic acids encoding multiprotein units and hosts including the expression cassettes are also provided. | 09-24-2015 |
20150353947 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 12-10-2015 |
20160024485 | USE OF DIMERIZATION DOMAINS FOR TEMPERATURE REGULATION OF ENZYME ACTIVITY - Methods for regulating protein activity by fusing dimerization domains to target protein are provided. Chimeric proteins that include dimerization domains fused to target proteins for altering the activity of the target proteins are described. Engineered nucleic acids encoding chimeric proteins and hosts engineered to express engineered nucleic acids are also provided. | 01-28-2016 |
Patent application number | Description | Published |
20090177438 | Apparatus and method for relocating an articulating-arm coordinate measuring machine - A measurement apparatus, system and method for measuring objects which is easily relocatable about the object is described. The system uses an articulated-arm coordinate measuring machine (CMM) and a laser tracker. A retroreflector for use with the laser tracker is located on the arm of the articulated-arm (CMM). A common coordinate frame of reference can be determined for the CMM and the laser tracker so that the CMM can be moved. Also, points hidden from the laser tracker can be measured for example with the CMM. | 07-09-2009 |
20090212669 | STORAGE UNIT WITH EXTENSION MECHANISM - A storage unit comprises an arm assembly that can be stored inside the unit and extended along a range of positions outside the unit and a moveable panel which conceals the interior of the storage unit frame coupled with the storage unit frame by a connector. The storage unit can be integrated into an article of furniture such as a headboard or a chair. A four-bar linkage assembly can be used to connect the moveable panel to the storage unit frame and can permit the panel to remain substantially parallel to the frame along its range of motion. | 08-27-2009 |
20100323128 | MIXED MEDIA ARTWORK AND METHODS OF CREATION - A method of making mixed media artwork includes cutting a metal sheet; applying an adhesive layer to an outer surface of the metal sheet; applying one or more polymer layers to the adhesive layer, wherein the one or more polymer layers create an image on the outer surface; and deforming the metal sheet to create a three-dimensional outer surface. | 12-23-2010 |
20120204436 | PORTABLE COORDINATE MEASUREMENT MACHINE WITH A ROTATABLE HANDLE - A portable articulated arm coordinate measurement machine (AACMM) includes a manually positionable articulated arm having opposed first and second ends, the articulated arm including a plurality of connected arm segments, each arm segment including at least one position transducer for producing a position signal resulting in the articulated arm having a plurality of position transducers. The AACMM also includes a base section connected to the second end; and a probe assembly connected to the first end, the probe assembly having a probe end, an inner core, and a handle; wherein the probe end is fixed with respect to the inner core, the handle is configured to rotate about the inner core, and the probe end is configured to measure a three-dimensional coordinate of a point in space. | 08-16-2012 |
20120222324 | PORTABLE COORDINATE MEASUREMENT MACHINE HAVING A HANDLE THAT INCLUDES ELECTRONICS - A portable articulated arm coordinate measurement machine, comprising: a manually positionable articulated arm having opposed first and second ends, the articulated arm including a plurality of connected arm segments, each arm segment including at least one position transducer for producing a position signal; a base section connected to the second end; and a probe assembly including a probe end and a handle; wherein the probe assembly is connected to the first end; wherein the handle contains electronics that include a processor; wherein the handle is configured to be gripped by a hand; and wherein the articulated arm coordinate measurement machine is configured to measure a three-dimensional coordinate of a point in space associated with the probe end. | 09-06-2012 |
20130097883 | PORTABLE COORDINATE MEASUREMENT MACHINE HAVING A REMOVABLE EXTERNAL SENSOR - A portable articulated arm coordinate measurement machine is provided. The coordinate measurement machine includes a manually positionable articulated arm having opposed first and second ends. The articulated arm includes a plurality of arm segments, each including encoder configured to produce a signal corresponding to an angle of rotation. A base section is connected to the second end. A measurement probe is connected to the first end. The measurement probe receives an externally mounted sensor having an attachment ring, the measurement probe having an alignment surface and external threading. A retaining ring having internal threading is threadably receives the external threading; the retaining ring further couples the attachment ring to the surface. An electrical circuit is configured to receive the signal and to provide data corresponding to a coordinate of the externally mounted sensor, the data based at least in part on the signal. | 04-25-2013 |
20130104411 | PORTABLE COORDINATE MEASUREMENT MACHINE HAVING A HANDLE THAT INCLUDES ELECTRONICS | 05-02-2013 |
20130129942 | MIXED MEDIA ARTWORK AND METHODS OF CREATION - A method of making mixed media artwork includes cutting a metal sheet; applying an adhesive layer to an outer surface of the metal sheet; applying one or more polymer layers to the adhesive layer, wherein the one or more polymer layers create an image on the outer surface; and deforming the metal sheet to create a three-dimensional outer surface. | 05-23-2013 |
20140172552 | SYSTEM AND METHOD FOR CLICK FRAUD PROTECTION - A system and method for preventing click fraud and/or determining invalid clicks are provided to measure click related data, decide according to target URL defined conditions whether to present an Intelligent interstitial, decide according to target URL defined conditions how to populate the interstitial and then measure Search user interaction with the interstitial. | 06-19-2014 |
20140278947 | SYSTEM AND METHOD FOR CLICK FRAUD PROTECTION - A system and method for preventing click fraud and/or determining invalid clicks are provided to measure click related data, decide according to target URL defined conditions whether to present an Intelligent interstitial, decide according to target URL defined conditions how to populate the interstitial and then measure Search user interaction with the interstitial. Other embodiments use target URL defined conditions of interstitial interaction to determine click validity and monetary value and in the event of a valid click continuing to the target URL and optionally measuring on-site behavior. Further embodiments measure data from an original click, observe interstitial behavior and optionally merge target URL website measured behavior into a database providing user data for each individual click. Other embodiments generate reports specific to seeking credits for payments on invalid clicks and other website intelligence. Exemplary embodiments provide for varying implementations, including e.g. implementations through search engines and standalone services. | 09-18-2014 |
20140324573 | SYSTEM AND METHOD FOR CLICK FRAUD PROTECTION - A system and method for preventing click fraud and/or determining invalid clicks are provided to measure click related data, decide according to target URL defined conditions whether to present an Intelligent interstitial, decide according to target URL defined conditions how to populate the interstitial and then measure Search user interaction with the interstitial. Other embodiments use target URL defined conditions of interstitial interaction to determine click validity and monetary value and in the event of a valid click continuing to the target URL and optionally measuring on-site behavior. Further embodiments measure data from an original click, observe interstitial behavior and optionally merge target URL website measured behavior into a database providing user data for each individual click. Other embodiments generate reports specific to seeking credits for payments on invalid clicks and other website intelligence. Exemplary embodiments provide for varying implementations, including e.g. implementations through search engines and standalone services. | 10-30-2014 |
20140358678 | SYSTEM AND METHOD FOR CLICK FRAUD PROTECTION - A system and method for preventing click fraud and/or determining invalid clicks are provided to measure click related data, decide according to target URL defined conditions whether to present an Intelligent interstitial, decide according to target URL defined conditions how to populate the interstitial and then measure Search user interaction with the interstitial. | 12-04-2014 |
20150032533 | SYSTEM AND METHOD FOR CLICK FRAUD PROTECTION - A system and method for preventing click fraud and/or determining invalid clicks are provided to measure click related data, decide according to target URL defined conditions whether to present an Intelligent interstitial, decide according to target URL defined conditions how to populate the interstitial and then measure Search user interaction with the interstitial. | 01-29-2015 |
20150046254 | SYSTEM AND METHOD FOR DISPLAY RELEVANCE WATCH - A system and method for relevancy of display placement, comprising a processor on non-transitory memory that compiles transactional information from advertisers, publishers and users and provides an interface that allows the advertiser to determine relevancy of the placement based on the activity of the user and publisher information. | 02-12-2015 |
20150068054 | PORTABLE COORDINATE MEASUREMENT MACHINE HAVING A HANDLE THAT INCLUDES ELECTRONICS - A portable articulated arm coordinate measurement machine, comprising: a manually positionable articulated arm having opposed first and second ends, the articulated arm including a plurality of connected arm segments, each arm segment including at least one position transducer for producing a position signal; a base section connected to the second end; and a probe assembly including a probe end and a handle; wherein the probe assembly is connected to the first end; wherein the handle contains electronics that include a processor; wherein the handle is configured to be gripped by a hand; and wherein the articulated arm coordinate measurement machine is configured to measure a three-dimensional coordinate of a point in space associated with the probe end. | 03-12-2015 |
20150192399 | PORTABLE COORDINATE MEASUREMENT MACHINE HAVING A HANDLE THAT INCLUDES ELECTRONICS - A portable articulated arm coordinate measurement machine having an articulated arm that includes a first arm connected to a second arm segment by a connecting segment. An assembly is provided having a first pair of bearings and a first optical encoder and a second pair of bearings and a second optical encoder The first optical encoder includes a first patterned disk and a first read head. The second optical encoder includes a second patterned disk and a second read head. The first and second patterned disks are both fixed with respect to the connecting segment. An electronic circuit is operably coupled to the first read head and the second read head, the electronics being configured to determine three-dimensional coordinates of the probe based at least in part on a first angle and a second angle measured by the first and second optical encoders. | 07-09-2015 |
Patent application number | Description | Published |
20080268838 | Mobile interactive satellite services - A system for providing mobile interactive satellite services includes a satellite operable to communicate with mobile units, a terrestrial base transceiver station operable to communicate with mobile units, and a ground station in communication with the satellite and the terrestrial base transceiver station to provide mobile interactive satellite services. The mobile interactive satellite services include a multicast component and an interactive component such that the ground station provides both the multicast component and the interactive component using the satellite, with the terrestrial base transceiver station used to provide an ancillary terrestrial component. A device for communicating with a mobile interactive satellite service system includes an antenna, a transceiver coupled to the antenna and operable to communicate with a mobile interactive satellite service system, a user input device, an output device, a processor unit, and a network interface. The processor unit is coupled to the user input device, the output device, and the transceiver such that the processor is operable to output received information from the transceiver using the output device, to receive interactive information from the user input device, and to transmit data based on the received interactive information using the transceiver. The network interface is coupled to the processor such that the processor is operable to side load information. | 10-30-2008 |
20130012238 | Method and System for Integrated Satellite Assistance Services - A method and an apparatus according to an embodiment of the invention includes a controller to process a travel assistance request for communication through a satellite and terrestrial mobile communication network. Data related to multiple assistance providers can be received in response to the request. At least one criterion can be processed for the assistance providers based on, for example, historical trip data. The assistance providers can be graphically represented for user selection based on the criterion. Data related to a selected assistance provider can be displayed, including navigation data to and/or from the assistance provider. In another embodiment, the travel assistance request can be communicated to a service center through the network. The service center can communicate data related to an assistance provider, including navigation data to and/or from the assistance provider. A rating of the assistance provider can be communicated to a specified community-of-interest through the network. | 01-10-2013 |
20130013193 | Method and System for Using Routine Driving Information in Mobile Interactive Satellite Services - A method and an apparatus according to an embodiment of the invention includes a controller to process a criterion for selecting a point-of-interest (POI). The criterion can be based on historical trip data. Data related to a POI, including a community-of-interest rating, for example, can be accessed through a satellite and terrestrial mobile communication network. Multiple POIs can be graphically represented for user selection based on the criterion and/or other data. Once a POI is selected, data related to the selected POI, including navigation data, can be displayed. In another embodiment, the controller can access navigation data to a specified destination. The navigation data can be based on historical trip data such as route segments and destination areas. The navigation data can be accessed from an in-vehicle system or from a navigation service provider and can be displayed to assist a user in reaching the specified destination. | 01-10-2013 |
20130189983 | Method and System for Integrated Assistance Service - A method and an apparatus according to an embodiment of the invention includes a controller to process a travel assistance request for communication through a satellite and terrestrial mobile communication network. Data related to multiple assistance providers can be received in response to the request. At least one criterion can be processed for the assistance providers based on, for example, historical trip data. The assistance providers can be graphically represented for user selection based on the criterion. Data related to a selected assistance provider can be displayed, including navigation data to and/or from the assistance provider. In another embodiment, the travel assistance request can be communicated to a service center through the network. The service center can communicate data related to an assistance provider, including navigation data to and/or from the assistance provider. A rating of the assistance provider can be communicated to a specified community-of-interest through the network. | 07-25-2013 |
20130197805 | Method and Sysem for Using Routine Driving Information in Mobile Interactive Services - A method and an apparatus according to an embodiment of the invention includes a controller to process a criterion for selecting a point-of-interest (POI). The criterion can be based on historical trip data. Data related to a POI, including a community-of-interest rating, for example, can be accessed through a satellite and terrestrial mobile communication network. Multiple POIs can be graphically represented for user selection based on the criterion and/or other data. Once a POI is selected, data related to the selected POI, including navigation data, can be displayed. In another embodiment, the controller can access navigation data to a specified destination. The navigation data can be based on historical trip data such as route segments and destination areas. The navigation data can be accessed from an in-vehicle system or from a navigation service provider and can be displayed to assist a user in reaching the specified destination. | 08-01-2013 |
20140095072 | METHOD AND SYSTEM FOR USING ROUTINE DRIVING INFORMATION IN MOBILE INTERACTIVE SATELLITE SERVICES - A method and an apparatus according to an embodiment of the invention includes a controller to process a criterion for selecting a point-of-interest (POI). The criterion can be based on historical trip data. Data related to a POI, including a community-of-interest rating, for example, can be accessed through a satellite and terrestrial mobile communication network. Multiple POIs can be graphically represented for user selection based on the criterion and/or other data. Once a POI is selected, data related to the selected POI, including navigation data, can be displayed. In another embodiment, the controller can access navigation data to a specified destination. The navigation data can be based on historical trip data such as route segments and destination areas. The navigation data can be accessed from an in-vehicle system or from a navigation service provider and can be displayed to assist a user in reaching the specified destination. | 04-03-2014 |
20150057881 | Method And System For Integrated Assistance Services - A method and an apparatus according to an embodiment of the invention includes a controller to process a travel assistance request for communication through a satellite and terrestrial mobile communication network. Data related to multiple assistance providers can be received in response to the request. At least one criterion can be processed for the assistance providers based on, for example, historical trip data. The assistance providers can be graphically represented for user selection based on the criterion. Data related to a selected assistance provider can be displayed, including navigation data to and/or from the assistance provider. In another embodiment, the travel assistance request can be communicated to a service center through the network. The service center can communicate data related to an assistance provider, including navigation data to and/or from the assistance provider. A rating of the assistance provider can be communicated to a specified community-of-interest through the network. | 02-26-2015 |
20150289123 | SYSTEM AND METHOD FOR ORCHESTRATING MOBILE DATA NETWORKS IN A MACHINE-TO-MACHINE ENVIRONMENT - A method is provided in one example and includes receiving network utilization data associated with an access network, determining an expected network utilization for a predetermined time period based upon the network utilization data, and determining pricing information for the predetermined time period based upon the expected network utilization. The pricing information includes at least one price rate for communication between at least one application and at least one client device utilizing the access network. The method further includes sending the pricing information to the at least one application. | 10-08-2015 |
Patent application number | Description | Published |
20100041052 | Receptor Tyrosine Kinase Assays - Methods for detecting phosphorylation of receptor tyrosine kinases (“RTKs”) upon activation are provided. The method employs cells comprising two fusion products: (1) an RTK fused to a small fragment of β-galactosidase and (2) a phosphotyrosine binding peptide fused to the large fragment of β-galactosidase, where the 2 fragments weakly complex to form an active enzyme, and optionally a construct for a cytosolic RTK phosphorylating kinase, when the RTK does not autophosphoryate. To detect phosphorylation a β-galactosidase substrate is added to the cells, whereby product formation indicates the occurrence of phosphorylation. | 02-18-2010 |
20100203555 | Wild-Type Receptor Assays - A method for determining ligand activation of receptors using cells expressing genetic constructs of a fusion protein of at least a binding domain of an auxiliary protein and a fragment of β-galactosidase, a fusion protein of an endosome-associated protein and a complementary fragment of β-galactosidase, and a wild-type receptor. The receptors are characterized by binding to the auxiliary protein-binding domain upon activation by an agonist and then endocytosing associated with an endosome to which the endosome-associated protein binds. Cells are incubated with a candidate ligand followed by lysis with a lysing medium comprising a substrate for the β-galactosidase. The enzyme product is then detected as a measure of the activation of the receptor. | 08-12-2010 |
20120040372 | RECEPTOR TYROSINE KINASE ASSAYS - Methods for detecting phosphorylation of receptor tyrosine kinases (“RTKs”) upon activation and the modulation of activation by a candidate compound are provided. The method employs cells comprising two fusion products: (1) an RTK fused to a small fragment of β-galactosidase and (2) a phosphotyrosine binding peptide fused to the large fragment of β-galactosidase, where the 2 fragments weakly complex to form an active enzyme, and optionally a construct for a cytosolic RTK phosphorylating kinase, when the RTK does not autophosphoryate. To detect phosphorylation a β-galactosidase substrate is added to the cells, whereby product formation indicates the occurrence of phosphorylation. | 02-16-2012 |
20120329075 | MONITORING PROTEIN TRAFFICKING USING BETA-GALACTOSIDASE REPORTER FRAGMENT COMPLEMENTATON - Methods and materials are disclosed for use in an enzyme fragment complementation assay using complementary fragments of β-galactosidase to study the trafficking of proteins in a cell. Compounds that bind to a target peptide have been found to affect protein folding and therefore trafficking. β-Galactosidase fragments, an enzyme donor (ED) and an enzyme acceptor (EA), are fused to a target peptide and to an intracellular compartment protein, wherein the compartment is involved in intracellular trafficking. Contacting the cell with a compound that binds to the target peptide results in enhanced movement of the protein through the cellular trafficking pathway comprised of the endoplasmic reticulum, Golgi apparatus, the plasma membrane, endosomes, etc. Using this approach, compounds that bind to a target peptide and alter its ability to traffic through the normal cellular pathway can be readily detected. | 12-27-2012 |
20130203067 | DETECTION OF INTRACELLULAR BINDING EVENTS BY MEASURING PROTEIN ABUNDANCE - Methods and compositions are provided to measure the binding of a test compound to a target peptide by measuring the effect of the compound on the abundance of the target peptide inside a cell. The target peptide may bind the test compound at an active site or an allosteric site, and it has been found that such binding may stabilize the target peptide against cellular degradation. The target peptide will preferably comprise a destabilizing mutation which shortens the half life of the target peptide within the cell, typically a mammalian cell. Test compounds, including small molecules, have been found to stabilize target peptides. Also provided are systems and kits for use in practicing the methods. | 08-08-2013 |