Quake
Stephen Quake, Aptos, CA US
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20140326400 | ROBUST MONOLITHIC MICROMECHANICAL VALVES FOR HIGH DENSITY MICROFLUIDIC VERY LARGE SCALE INTEGRATION - A fabrication method of a micromechanical valve includes: (1) forming a control layer according to a first weight ratio of cross linker: elastomer base; (2) forming a flow layer according to a second weight ratio of cross linker: elastomer base; (3) forming a membrane layer according to a third weight ratio of cross linker: elastomer base, where the third weight ratio is smaller than the first weight ratio, and is smaller than the second weight ratio; (4) bonding the membrane layer to the control layer to form a two-layer structure; and (5) bonding the two-layer structure to the flow layer to form the micromechanical valve. | 11-06-2014 |
Stephen Quake, San Marino, CA US
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20110275523 | METHODS AND KITS FOR ANALYZING POLYNUCLEOTIDE SEQUENCES - The present invention features methods for analyzing a sequence of a target polynucleotide by detecting incorporation of a nucleotide into its complementary strand, where the polynucleotides may be bound at high density and at single molecule resolution. The invention also features labeling moieties and blocking moieties, which facilitate chain termination or choking. Certain aspects provide for temporal detection of the incorporations; some allow for asynchronous analysis of a plurality of target polynucleotides and the use of short sequencing cycles. Surface chemistry aspects of the sequencing methods are also provided. The method may also be used in kits, said kits designed to carry out and facilitate the methods provided herein. | 11-10-2011 |
Stephen R. Quake, New York, NY US
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20130011618 | PHOTOCURABLE PERFLUOROPOLYETHERS FOR USE AS NOVEL MATERIALS IN MICROFLUIDIC DEVICES - A functionalized photocurable perfluoropolyether is used as a material for fabricating a solvent-resistant microfluidic device. Such solvent-resistant microfluidic devices can be used to control the flow of small amounts of a fluid, such as an organic solvent, and to perform microscale chemical reactions that are not amenable to other polymer-based microfluidic devices. | 01-10-2013 |
Stephen R. Quake, Singapore SG
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20130011833 | METHOD FOR IDENTIFYING NUCLEIC ACIDS BOUND TO AN ANALYTE - A method for identifying a target nucleic acid bound by an analyte in a sample comprising: (a) contacting said sample with a first probe comprising a first nucleic acid and a first analyte binding domain and a second probe comprising a second nucleic acid and second analyte binding domain, wherein said first and second probes can bind to said analyte, such that said first and second nucleic acid are in spatial proximity to form a complex with said target nucleic acid if said target nucleic acid is bound by said analyte in said sample; (b) incubating said sample with a ligase that can ligate said complex to form a ligated target nucleic acid template; (c) amplifying said target nucleic acid template if present in said sample, and (d) detecting the presence or absence of an amplified target nucleic acid template. | 01-10-2013 |
Stephen R. Quake, Palo Alto, CA US
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20150368670 | COMPOSITIONS AND METHODS OF DELIVERING TREATMENTS FOR LATENT VIRAL INFECTIONS - The invention provides delivery methods and compositions for antiviral therapeutics. Methods and compositions are provided for targeted delivery of antiviral therapeutics into cells of interest using, for example, viral vectors such as adenovirus, AAV, and replication incompetent HSV. These and other delivery systems can be used as vehicles to deliver DNA vectors encoding a nuclease or a cell-killing gene. These delivery methods can also be used to deliver naked DNA or RNA, protein products, plasmids containing a promoter that is active only in a latent viral state which drives a cell-killing gene, or other therapeutic agents. | 12-24-2015 |
Stephen R. Quake, Pasadena, CA US
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20090035838 | Microfabricated Crossflow Devices and Methods - A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly. | 02-05-2009 |
Stephen R. Quake, San Mateo, CA US
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20090151422 | MICROFABRICATED ELASTOMERIC VALVE AND PUMP SYSTEMS - A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate. | 06-18-2009 |
Tom D. Quake, Three Rivers, MI US
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20080270047 | Method and Apparatus for Analyzing Steam Trap Data - A system for analyzing steam trap data includes a steam trap monitoring device operable to monitor a parameter of a steam trap, transform the parameter into data, and transmit the data to a computer; and includes a computer operable to receive the data, the computer including an algorithm for evaluating the data. A method of analyzing steam trap data includes the steps of: providing a steam trap monitoring device operable to monitor a parameter of a steam trap, transform the parameter into data, and transmit data to a computer, and providing a computer operable to receive the data, the computer including an algorithm for evaluating the data; using the steam trap monitoring device to monitor a parameter of a steam trap, transform the parameter into data, and transmit the data to the computer; and using the computer to receive the data, and evaluate the data with the algorithm. | 10-30-2008 |