Patent application number | Description | Published |
20090204337 | Method for Identifying the Elution Time of an Analyte - A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions. | 08-13-2009 |
20090326828 | Optimizing Selection of SRM Transitions for Analysis of Biomolecules by Tandem Mass Spectrometry - Methods for selecting a set of SRM transitions for a peptide of interest include selecting a first transition based on sensitivity criteria and selecting at least a second transition based on selectivity criteria. A determination of the uniqueness of the first transition combined with the at least a second transition is made. When the combination of the first transition and the at least a second transition is determined to be unique to the peptide of interest, a sample containing the peptide of interest is subjected to a SRM workflow by monitoring the first transition and the second transition. Also described is an apparatus for carrying out the methods. | 12-31-2009 |
20100216250 | Methods for Predicting Trisomy 21 in a Fetus - The present invention relates to biomarkers in maternal serum samples and methods for predicting whether a fetus has Trisomy 21 during the first trimester of pregnancy based on the differential expression of a combination, or subcombination, of the same. | 08-26-2010 |
20110114836 | Method for Identifying the Elution Time of an Analyte - A method for determining a time of elution of a peptide of interest from a liquid chromatography column includes a step of obtaining chromatographic data for each of a plurality of candidate fragment ions of the peptide of interest. A time along a common chromatographic time is scale determined corresponding to maximum overlay of the ion signals measured for each of the plurality of candidate fragment ions. Finally, the determined time is assigned as the time of elution of the peptide of interest from the liquid chromatography column. In particular, the chromatographic data is acquired during selective reaction monitoring of an eluate from the liquid chromatography column containing the peptide of interest. The chromatographic data includes ion signals measured along the common chromatographic time scale for each of the plurality of candidate fragment ions. | 05-19-2011 |
20110151568 | Assay for Monitoring Parathyroid Hormone (PTH) Variants by Tandem Mass Spectrometry - Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay. | 06-23-2011 |
20120064558 | Assays for Differentiating between Ischemic and Hemorrhagic Stroke in a Human Patient - Methods are disclosed for rapidly and reliably differentiating between ischemic and hemorrhagic stroke events in a human patient. The methods include obtaining a biological sample, such as blood serum, and analyzing the sample or a derivative thereof by mass spectrometry to measure the amount of one or more targeted proteins differentially present and ischemic and hemorrhagic stroke patients. The analysis may include proteolytic digestion of the biological sample and selective reaction monitoring (SRM) tandem mass spectrometry of the digested sample to determine the amounts of surrogate peptides corresponding to the targeted proteins. | 03-15-2012 |
20130217058 | Assay for Monitoring Parathyroid Hormone (PTH) Variants by Tandem Mass Spectrometry - Methods are described for monitoring the amounts of PTH variants in a biological sample by digesting the sample to produce surrogate peptides specific to the targeted PTH variants, and detecting and quantifying the surrogate peptides by selective reaction monitoring (SRM) mass spectrometry, using a set of precursor-to-product ion transitions optimized for sensitivity and selectivity. The PTH variants, or a portion thereof, may be concentrated in the sample by means of immunoaffinity capture or other suitable technique. The mass spectrometric method described herein enables the concurrent measurement of peptides representative of a plurality of targeted PTH variants in a single assay. | 08-22-2013 |
Patent application number | Description | Published |
20090311332 | Method for forming mesoporous silica nanoparticles, mesoporous silica nanopartices, and applications thereof - A method for synthesizing a mesoporous silica nanoparticle, a mesoporous silica nanoparticle, and applications thereof are provided. The method includes fractionating a mesoporous silica nanoparticle suspension to produce size-fractionated mesoporous silica nanoparticle. The method further includes etching the size-fractionated mesoporous silica nanoparticle to produce synthesized mesoporous silica nanoparticle having a hollow, porous morphology configured to receive one of a therapeutic agent and an imaging material. The etching includes differential etching of silica from areas of low polymeric density within the mesoporous silica nanoparticle and re-depositing of the silica in areas of higher polymeric density existing near the surface of the mesoporous silica nanoparticle. A target material is loaded into the synthesized mesoporous silica nanoparticle and a controlled released of the target material is provided by decreasing the physiological pH of the surface of the mesoporous silica nanoparticle. | 12-17-2009 |
20110313094 | Carbon Blacks Having Low PAH Amounts And Methods Of Making Same - Carbon blacks, such as rubber blacks, having a low PAH concentration are described. Furthermore, elastomeric or rubber compositions containing the carbon black of the present invention are further described, as well as methods of making carbon black having a low PAH concentration. | 12-22-2011 |
20160075836 | Elastomer Composites, Blends and Methods for Preparing Same - Blends of elastomer composites with unfilled or less highly filled elastomer reduces hysteresis without compromising reinforcement. Hysteresis may also be reduced by adjusting the compounding method. | 03-17-2016 |
Patent application number | Description | Published |
20090156427 | Bio-Sensing Nanodevice - The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor. | 06-18-2009 |
20110048543 | Microfluidic Bubble Logic Devices and Methods - A method for implementing a logic operation employs an all fluid-based no-moving part micro-mechanical logic family of microfluidic bubble logic devices that are constructed from complex sequences of microfluidic channels, microfluidic bubble modulators for programming the devices, and microfluidic droplet/bubble memory elements for chemical storage and retrieval. The input is a sequence of bubbles/droplets encoding information, with the output being another sequence of bubbles/droplets. For performing a set of reactions/tasks, the modulators program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the microfluidic channel sequence, utilizing the generated bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries. Various devices, including logic gates, non-volatile bistable memory, shift registers, multiplexers, and ring oscillators have been designed and fabricated. | 03-03-2011 |
20110275143 | Microfluidic Bubble Logic Devices - Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries. Various devices, including logic gates, non-volatile bistable memory, ring oscillators, bubble synchronizers, analysis chips, and printers have been designed. | 11-10-2011 |
20120291870 | Microfluidic Bubble Logic Devices and Methods - A method for implementing a logic operation employs an all fluid-based no-moving part micro-mechanical logic family of microfluidic bubble logic devices that are constructed from complex sequences of microfluidic channels, microfluidic bubble modulators for programming the devices, and microfluidic droplet/bubble memory elements for chemical storage and retrieval. The input is a sequence of bubbles/droplets encoding information, with the output being another sequence of bubbles/droplets. For performing a set of reactions/tasks, the modulators program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the microfluidic channel sequence, utilizing the generated bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries. Various devices, including logic gates, non-volatile bistable memory, shift registers, multiplexers, and ring oscillators have been designed and fabricated. | 11-22-2012 |
20130146459 | MULTIPHASE NON-LINEAR ELECTROKINETIC DEVICES - This invention provides devices and apparatuses comprising the same and methods of use thereof for efficient pumping and/or mixing of relatively small volumes of fluid, wherein the fluid contains a sample within an inner fluid phase dispersed in an outer phase. Such devices utilize nonlinear electrokinetics as a primary mechanism for driving fluid flow and/or mixing the fluid. Methods of cellular analysis, drug delivery and others, utilizing the devices are described. | 06-13-2013 |
20130167958 | Microfluidic Bubble Logic Devices - Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries. Various devices, including logic gates, non-volatile bistable memory, ring oscillators, bubble synchronizers, analysis chips, and printers have been designed. | 07-04-2013 |
20150011404 | Microfluidic Bubble Logic Devices - Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries. Various devices, including logic gates, non-volatile bistable memory, ring oscillators, bubble synchronizers, analysis chips, sample collectors, and printers have been designed. | 01-08-2015 |
Patent application number | Description | Published |
20140080725 | METHOD FOR SEPARATION AND DETECTION OF DNA FRAGMENTS - A method of detecting nucleic acid fragments is provided. The method includes providing a plurality of chambers, each chamber separated from the other chambers of the plurality, each chamber having at least one set of probes disposed therein, each set of probes being capable of binding to a different target nucleic acid sequence relative to the other sets of probes. The method also includes providing a sample comprising a plurality of sets of nucleic acid fragments, placing at least a portion of the sample into each of the plurality of chambers and causing the at least one set of probes in each chamber to bind with complementary nucleic acid fragments of the sample, and detecting the binding of the nucleic acid fragments to the sets of probes in each chamber. | 03-20-2014 |
20140080726 | ENHANCED METHOD FOR PROBE BASED DETECTION OF NUCLEIC ACIDS - A method of detecting nucleic acid fragments is provided. The method includes providing a plurality of sets of probes, each set of probes having a nucleic acid sequence. A first portion of the sequence is complementary to a target nucleic acid sequence, which differs for each set of probes relative to the other sets of probes. A second portion of the sequence is a specified sequence, which is the same for each set of probes. Each probe has a fluorescent label joined to the second portion of the sequence. The first portion of the sequence binds with nucleic acid fragments of a sample having a sequence complementary to said first portion. A quenching compound that has a quenching moiety and a nucleic acid sequence complementary to the specified sequence of the second portion of the sets of probes quenches the fluorescence. | 03-20-2014 |
20140200167 | FUNCTIONALLY INTEGRATED DEVICE FOR MULTIPLEX GENETIC IDENTIFICATION - A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of DNA fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material. The biochip also includes a plurality of separation and detection chambers that physically separate different DNA fragments that have been marked with labels that emit similar colors, thereby enabling the independent detection of the different DNA fragments. | 07-17-2014 |
20140328733 | Simplified Gating Method for Sealing and Flow Control in Micro and Nano Devices - A biochip for multiplex genetic identification is disclosed. An biochip for separating and detecting a plurality of DNA fragments includes a set of inputs and chambers for receiving a sample matrix of genetic material and reagents needed to conduct a polymerase chain reaction amplification of the genetic material. The biochip also includes a plurality of separation and detection chambers that physically separate different DNA fragments that have been marked with labels that emit similar colors, thereby enabling the independent detection of the different DNA fragments. | 11-06-2014 |