Patent application number | Description | Published |
20120200694 | MULTI-MODALITY CONTRAST AND BRIGHTFIELD CONTEXT RENDERING FOR ENHANCED PATHOLOGY DETERMINATION AND MULTI-ANALYTE DETECTION IN TISSUE - Multiple modality contrast can be used to produce images that can be combined and rendered to produce images similar to those produced with wavelength absorbing stains viewed under transmitted white light illumination. Images obtained with other complementary contrast modalities can be presented using engineered color schemes based on classical contrast methods used to reveal the same anatomical structures and histochemistry, thereby providing relevance to medical training and experience. Dark-field contrast images derived from refractive index and fluorescent DAPI counterstain images are combined to produce images similar to those obtained with conventional H&E staining for pathology interpretation. Such multi-modal image data can be streamed for live navigation of histological samples, and can be combined with molecular localizations of genetic DNA probes (FISH), sites of mRNA expression (mRNA-ISH), and immunohistochemical (IHC) probes localized on the same tissue sections, used to evaluate and map tissue sections prepared for imaging mass spectrometry. | 08-09-2012 |
20120214195 | METHOD FOR TISSUE SAMPLE FIXATION - An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed. | 08-23-2012 |
20120329088 | PROCESSING SYSTEM FOR PROCESSING SPECIMENS USING ACOUSTIC ENERGY - A method for fixing a biological sample includes delivering energy through a biological sample that has been removed from a subject, while fixing the biological sample. A change in speed of the energy traveling through the biological sample is evaluated to monitor the progress of the fixation. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. A computing device can evaluate the speed of the energy based on signals from the receiver. | 12-27-2012 |
20130171642 | AUTOMATED ANALYSIS OF CIRCULATING TUMOR CELLS - The disclosure provides methods for automated characterization of circulating tumor cells (CTCs), for example using automated tissue strainers. In specific examples, such methods permit characterizing a prostate cancer sample by simultaneously or contemporaneously detecting ERG rearrangements and PTEN deletions in the same CTC. Also provided are kits that can be used with such methods. | 07-04-2013 |
20130203100 | OPPOSABLES AND AUTOMATED SPECIMEN PROCESSING SYSTEMS WITH OPPOSABLES - A specimen processing system is capable of processing specimens carried on slides. The specimen processing system can sequentially deliver slides and opposables to specimen processing stations. The specimen processing stations can use the opposables to apply a series of liquids to the specimens. The applied liquid can be moved along the slide using capillary action while the specimen processing stations control the processing temperatures. The applied liquid can be in a fluid-carrying gap. The opposable can contact the slide to vary a cross section of the fluid-carrying gap. | 08-08-2013 |
20130224791 | SYSTEMS AND METHODS FOR MONITORING TISSUE SAMPLE PROCESSING - A tissue sample that has been removed from a subject can be evaluated. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The monitoring can detect movement of fluid with the sample and cross-linking. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy. | 08-29-2013 |
20140078286 | METHOD AND SYSTEM FOR SPECTRAL UNMIXING OF TISSUE IMAGES - A method and system for spectral demultiplexing of fluorescent species, such as quantum dots, conjugated with a biological tissue. The process of demultiplexing involves a non-liner regression based on curve-fitting of estimated spectra of the quantum dots and confidence intervals describing the parameters of such fitting curve for typical quantum dots. | 03-20-2014 |
20140252200 | POLYFOCAL INTERFEROMETRIC IMAGE ACQUISTION - A microscope-based system and method for simultaneous imaging of several object planes, of a three-dimensional (3D) sample, associated with different depths throughout the sample. The system includes a polyfocal optical portion, adapted to create a plurality of optical channels each of which is associated with an image of a corresponding object plane, and a spectrally-selective portion, adapted to transform the spectral distribution of the image-forming beam of light to a corresponding spatial distribution. The image, registered by a detector, includes an image of an object plane and an image of the spatially-coded spectral distribution. The method effectuates the simultaneous multispectral imaging of the several object planes. The required data-acquisition time is several fold shorter than that taken by a conventional multispectral microscope-based imaging system. | 09-11-2014 |
20140296088 | Spectral Imaging for Measurement of Nuclear Pathology Features in Cancer Cells Prepared for In Situ Analysis - In general, the presently disclosed technology relates to identification of cancer subtypes. More specifically, the technology relates to methods for determining molecular drivers of cancer and/or progression using a multivariate image data and statistical analysis of in-situ molecular markers and morphological characteristics in the same cells of a biological sample suspected of b cancer. This analysis takes place after a single acquisition that obtains the molecular and anatomic morphology data in parallel. The analysis compares specific morphological and molecular markers to known samples exhibiting particular genetic drivers of the cancer. This method provides statistical information that allows for an increased confidence in the identification of specific molecular drivers of the cancer. | 10-02-2014 |
20150024429 | METHOD AND DEVICE FOR THE HOMOGENEOUS DISTRIBUTION OF SUSPENDED CELL COMPONENTS - A method for the homogeneous distribution of cell components suspended in a liquid on a surface and a device for the implementation thereof. | 01-22-2015 |
20150197811 | Spectral Imaging for Measurement of Nuclear Pathology Features in Cancer Cells Prepared for In Situ Analysis - In general, the presently disclosed technology relates to identification of cancer subtypes. More specifically, the technology relates to methods for determining molecular drivers of cancer and/or progression using a multivariate image data and statistical analysis of in-situ molecular markers and morphological characteristics in the same cells of a biological sample suspected of b cancer. This analysis takes place after a single acquisition that obtains the molecular and anatomic morphology data in parallel. The analysis compares specific morphological and molecular markers to known samples exhibiting particular genetic drivers of the cancer. This method provides statistical information that allows for an increased confidence in the identification of specific molecular drivers of the cancer. | 07-16-2015 |
Patent application number | Description | Published |
20090070087 | Virtual tissue with emergent behavior and modeling method for producing the tissue - A multi-cellular virtual tissue having the emergent properties of self-repair, adaptive response to an altered environment, or tissue differentiation, and a method of generating the tissue by computer modeling are disclosed. The tissue is formed of a plurality of virtual cells, each having a heritable virtual genome containing a set of virtual genes relating to each of (a1) intercellular adhesion, (a2) cell division, (a3) cell growth, (a4) intercellular signaling, and (a5) the state of one cell relative to an adjacent cell. In forming the tissue, the sequential operation and actions of the genes are guided by (1) chemical-interaction rules that govern the extra-genetic behavior of one or more molecules placed or produced in the environment, (2) action rules that specify a cell's adhesion, growth, or cell-division condition, in response to molecules produced by a cell's genes relating to intercellular adhesion, cell growth, or cell division, respectively, and (3) physical-interaction rules that govern how a cell will move in response to its own growth or division or the growth or division of neighboring cells. | 03-12-2009 |
20100138204 | CELL-BASED MODELS AND METHODS FOR SIMULATING LYMPHOCYTE DIFFERENTIATION AND OTHER BIOLOGICAL EVENTS - Systems and methods are disclosed herein that enable computer-implemented modeling of lymphocyte differentiation and developmental processes. Cell-based models and methods for simulating natural and transgenic lymphocyte differentiation are also disclosed. In some embodiments, systems and methods are provided for cell-centric simulation of lymphocyte differentiation with accommodating virtual thymic and/or bone marrow environment feedback. In one embodiment, a computer-implemented method of modeling lymphocyte differentiation can include receiving configurable simulation information and initializing an ontogeny engine to an initial step boundary in accordance with the configurable simulation information. The method can also include advancing the ontogeny engine, until a halting condition is encountered, from a current step boundary to a next step boundary in accordance with the configurable simulation information and the current step boundary. The initial step boundary can define at least one virtual early lymphoid progenitor cell. The advancing can include performing a stepCells function. | 06-03-2010 |
20100153082 | SYSTEMS AND METHODS FOR CELL-CENTRIC SIMULATION OF BIOLOGICAL EVENTS AND CELL BASED-MODELS PRODUCED THEREFROM - Systems and methods are provided herein that enable computer-implemented modeling of a biological event. Cell-based models produced from such systems and methods are also disclosed. In some embodiments, systems and methods are provided for cell-centric simulation with accommodating environment feedback. In one embodiment, a computer-implemented method of modeling a biological event can include receiving configurable simulation information and initializing an ontogeny engine to an initial step boundary in accordance with the configurable simulation information. The method can also include advancing the ontogeny engine from a current step boundary to a next step boundary in accordance with the configurable simulation information and the current step boundary. The advancing can include performing a metabolizeCell function. The method can further include continuing the advancing until a halting condition is encountered. In some embodiments, simulation of biological events includes modeling biological processes, such as development of ECM, multicellular tissue and differentiation of pluripotent cells. | 06-17-2010 |
20100305929 | SYSTEMS AND METHODS FOR CELL-CENTRIC SIMULATION AND CELL-BASED MODELS PRODUCED THEREFROM - Systems and methods are provided herein that enable computer-implemented modeling of a biological event. Cell-based models produced from such systems and methods are also disclosed. In some embodiments, systems and methods are provided for cell-centric simulation with accommodating environment feedback. In one embodiment, a computer-implemented method of modeling a biological event can include receiving configurable simulation information and initializing an ontogeny engine to an initial step boundary in accordance with the configurable simulation information. The method can also include advancing the ontogeny engine from a current step boundary to a next step boundary in accordance with the configurable simulation information and the current step boundary. The advancing can include performing a stepCells function. The method can further include continuing the advancing until a halting condition is encountered. In some embodiments, simulation of biological events includes modeling biological processes, such as development of multicellular tissue and differentiation of pluripotent cells. | 12-02-2010 |