Patent application number | Description | Published |
20090246885 | Media for affinity chromatography - The invention relates generally to solid supports for chromatography. In specific embodiments the invention provides for solid supports suitable for affinity chromatography along with methods, systems and kits which use the same. | 10-01-2009 |
20100063261 | Affinity chromatography matrices and methods of making and using the same - The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule. | 03-11-2010 |
20100174051 | Asymmertric porous adsorptive bead - The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead. | 07-08-2010 |
20100221844 | Caustic stable chromatography ligands - The present invention relates to chromatography ligands having improved caustic stability, e.g., ligands based on immunoglobulin-binding proteins such as, | 09-02-2010 |
20110065901 | Methods For Purifying A Target Protein From One or More Impurities In A Sample - The present invention relates, at least in part, to improved methods of protein purification. In particular, the present invention relates, at least in part, to methods for purifying an Fc region containing protein from a composition comprising the Fc region containing protein and one or more impurities, where the methods eliminate the need for a holding tank and/or a buffer exchange step. | 03-17-2011 |
20110105730 | AFFINITY CHROMATOGRAPHY MATRICES AND METHODS OF MAKING AND USING THE SAME - The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule. | 05-05-2011 |
20110313066 | STIMULUS RESPONSIVE POLYMERS FOR THE PURIFICATION OF BIOMOLECULES - The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules. | 12-22-2011 |
20120156757 | PURIFICATION OF IMMUNOGENS USING A NON-POLYSACCHARIDE MATRIX - The present invention relates, at least in part, to novel and improved chromatography matrices for separating or purifying immunogens, such as, for example, viruses and viral surface proteins, from one or more contaminants in a sample, where the matrix comprises a porous non-polysaccharide solid support comprising a negatively charged, multivalent ion exchange group directly attached to the solid support. | 06-21-2012 |
Patent application number | Description | Published |
20100044302 | Hydrophilic, high protein binding, low fluorescence, western blotting membrane - Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use. | 02-25-2010 |
20110045493 | Hydrophilic, High Protein Binding, Low Fluorescence, Western Blotting Membrane - Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use. | 02-24-2011 |
Patent application number | Description | Published |
20130225701 | GRAFTING METHOD TO IMPROVE CHROMATOGRAPHY MEDIA PERFORMANCE - The invention relates to improved methods of grafting polymer extenders onto porous substrates having diffusive pores, such as those used in protein separations, without filing the diffusive pores of the substrate, and restricting diffusion there through. By changing the grafting conditions and/or monomer composition(s) the resulting porous substrates having polymer extenders grafted thereto have increased protein binding capacity and resin selectivity, thereby enhancing the protein separation effectiveness of the substrate. The grafted polymer extenders provide the substrate with significant binding capacity at higher conductivity. The invention also relates to kits, and methods of using and grafting polymer extenders on porous resin substrates having diffusive pores. | 08-29-2013 |
20140073769 | ASYMMETRIC POROUS ADSORPTIVE BEAD - The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead. | 03-13-2014 |