Patent application number | Description | Published |
20090136995 | Mutated Prokaryotic Cells with High Secretion-Levels - A mutated prokaryotic cell, which secretes higher amounts of at least one heterologous polypeptide of interest and which has a reduced expression-level of YusZ or YusX, or homologues thereof, when compared with an otherwise isogenic but non-mutated cell, and methods for constructing and using such a cell in the production of polypeptides. | 05-28-2009 |
20090275104 | Bacillus licheniformis chromosone - The present invention relates to an isolated polynucleotide of the complete chromosome of | 11-05-2009 |
20100075376 | DNase Expression in Recombinant Host Cells - The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of: (a) cultivating a cell that produces at least one polypeptide of interest and expresses one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s); and (b) isolating the polypeptide of interest. | 03-25-2010 |
20100151521 | Method for Stable Gene-Amplification in a Bacterial Host Cell - A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention. | 06-17-2010 |
20110086407 | Bacillus licheniformis chromosome - The present invention relates to an isolated polynucleotide of the complete chromosome of | 04-14-2011 |
20120070881 | Bacillus licheniformis chromosome - The present invention relates to an isolated polynucleotide of the complete chromosome of | 03-22-2012 |
20120264169 | DNASE EXPRESSION IN RECOMBINANT HOST CELLS - The present invention relates to cells producing at least one polypeptide of interest and expressing one or more recombinant nuclease encoding gene(s) thereby producing the nuclease(s), and methods for producing a polypeptide of interest essentially free from contaminating DNA, said method comprising the steps of:
| 10-18-2012 |
20130217097 | CELL WITH IMPROVED SECRETION MEDIATED BY MRGA PROTEIN OR HOMOLOGUE - A progeny cell derived from a parent cell, wherein the progeny cell comprises at least one gene encoding MrgA protein or a functional homologue thereof and/or a DNA segment operably linked with the encoding gene, wherein said gene and/or DNA segment is manipulated with respect to the parent cell; the progeny cell comprises two or more copies of a gene encoding MrgA protein or a functional homologue thereof; or the progeny cell is mutated with respect to the parent cell; whereby the progeny cell produces greater amounts of MrgA protein or a functional homologue thereof than the parent cell. | 08-22-2013 |
20140178930 | Method for Stable Gene-Amplification in a Bacterial Host Cell - A bacterial host cell is disclosed including at least two copies of an amplification unit in its genome, the amplification unit including: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of the conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention. | 06-26-2014 |
20140273237 | Stable Genomic Integration of Multiple Polynucleotide Copies - Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells. | 09-18-2014 |