Patent application number | Description | Published |
20090023608 | METHODS AND APPARATUS FOR CELL CULTURE ARRAY - Method and systems that provide improved handling and/or culturing and/or assaying of cells, chemically active beads, or similar materials in microfluidic systems and microfluidic culture arrays. | 01-22-2009 |
20090213369 | Metallic Nanostructures Adapted for Electromagnetic Field Enhancement - The disclosure relates to metallic nanophotonic crescent structures, or “nanocrescent SERS probes,” that enhance detectable signals to facilitate molecular detections. More particularly, the nanocrescent SERS probes of the disclosure possess specialized geometries, including an edge surrounding the opening that is capable of enhancing local electromagnetic fields. Nanosystems utilizing such structures are particularly useful in the medical field for detecting rare molecular targets, biomolecular cellular imaging, and in molecular medicine. | 08-27-2009 |
20090314929 | Biomimetic Microfabricated Compound Eyes - An artificial compound eye comprising a plurality of three-dimensional (3D) self-aligned polymer microlenses disposed on a curvilinear surface; and a plurality of waveguides, wherein each of the waveguides is in optical communication with one of the plurality of polymer microlenses. | 12-24-2009 |
20100003666 | Microfluidic Methods for Diagnostics and Cellular Analysis - Methods for detection of molecular recognition and analysis of cells are provided. Both optical and non-optical methods are presented. Methods utilize capture of particles in semi-permeable structures. Specific microfluidic system architectures for conducting biomolecule and cell assays are described. | 01-07-2010 |
20100013115 | Apparatus and Method for Forming Fibers - A method and apparatus for forming materials such as fiber and elongated shapes, including: a tapered flow channel; supply channels for the addition and removal of agents into the tapered flow channel; and a fiber outlet at the distal end of the tapering flow channel. | 01-21-2010 |
20100048412 | DETECTION OF PROTEASE AND PROTEASE ACTIVITY USING A SINGLE NANOSCRESCENT SERS PROBE - This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide. | 02-25-2010 |
20100105053 | APTAMER BASED SENSORS AND RELATED METHODS AND SYSTEMS - An aptamer based-sensor comprising: a target binding aptamer attaching a Raman probe and a metal coated surface; and related methods and systems | 04-29-2010 |
20100136551 | MICROFLUIDIC PLATFORM AND RELATED METHODS AND SYSTEMS - A microfluidic platform comprising one or more chambers connectable through microfluidic channels, and comprising a substrate presenting aptamer sensors detectable through Raman active molecules, and related methods and systems. | 06-03-2010 |
20100196920 | NANOSCOPIC BIOMOLECULAR ABSORPTION SPECTROSCOPY ENABLED BY SINGLE NANOPARTICLE PLASMON RESONANCE ENERGY TRANSFER - The disclosure provides methods and compositions useful for measuring a target analyte in a sample with nanoparticle plasmon resonance. In particular the disclosure provides methods and compositions for measuring a target analyte comprising plasmon resonance energy transfer. | 08-05-2010 |
20100323906 | NANOPLASMONIC MOLECULAR RULER FOR NUCLEASE ACTIVITY AND DNA FOOTPRINTING - This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplamonic ruler provides a fast and convenient platform for mapping nucleic acid -protein interactions, for nuclease activity monitoring, and for other footprinting related methods. | 12-23-2010 |
20110026024 | METHOD AND APPARATUS FOR DETECTING METAL IONS, PROBE USED FOR THE SAME AND PREPARATION METHOD THEREOF - Disclosed is an innovative method for detecting metal ions based on selective plasmonic resonance energy transfer between metal-ligand complexes and a single nanoplasmonic particle as a probe. The selective plasmonic resonance energy transfer occurs if a resonance frequency matching condition between the single nanoplasmonic particle and the metal-ligand complexes is satisfied. | 02-03-2011 |
20110249267 | PLASMONIC DROPLET, METHOD AND APPARATUS FOR PREPARING THE SAME, DETECTION METHOD USING PLASMONIC DROPLET - Disclosed herein is an innovative plasmonic droplet including a droplet of fluid, a detection-target material which is in the droplet of fluid, and a nanoplasmon probe which is on a surface of the droplet of fluid and/or in the droplet of fluid. The plasmonic droplet may be applied for plasmon based optical sensing techniques, for example, for ultrasensitive analysis of bacteria, pathogen, etc. | 10-13-2011 |
20120135887 | CELL HANDLING, ELECTROPORATION AND ELECTROFUSION IN MICROFLUIDIC SYSTEMS - Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion. | 05-31-2012 |
20120258488 | Systems and Methods for Electrophysiological Activated Cell Sorting and Cytometry - Provided herein are methods and systems for non-genetic, label-free cell purification (i.e., cell cytometry and sorting), which classifies cells based on their spontaneous electrophysiological response or their electrophysiological response to a stimulus. For example, in one embodiment, there is provided a method of cell sorting comprising: stimulating a cell with a stimulus; sensing a response evoked by the cell based on the stimulus; identifying a phenotype of the cell based on the evoked response; and sorting the cell based on its phenotype. In one embodiment, the stimulus may be an electrical stimulus, a mechanical stimulus, an optical stimulus, a thermal stimulus, a chemical stimulus, or any combination thereof. The cell phenotype may be, for example, cardiomyocytes, neurons, smooth muscle cells, or pancreatic beta cells. | 10-11-2012 |
20130196435 | STENCIL PATTERNING METHODS AND APPARATUS FOR GENERATING HIGHLY UNIFORM STEM CELL COLONIES - A method for producing highly uniform cell colonies in a cell culture dish with the use of stencils made from an elastomeric sheet sized to fit within the cell culture dish, having a singular opening or a plurality of openings of a number, pitch and diameter configured to optimally control the geometric growth parameters of a cell colony. The uniform cell colonies are produced by placing the stencil in a cell culture dish and hydropilizing the stencil. The stencil is overlayed with cell culture media and seeded with seed cells that are preferably grown for at least a day before the stencil is removed to produce a pattern of seeded cells with controlled pitch, colony diameter and density within the culture dish that grow to become highly uniform cell colonies. A kit with culture dish, stencil, culture media and growth media is also provided. | 08-01-2013 |
20140011705 | DETECTION OF PROTEASE AND PROTEASE ACTIVITY USING A SINGLE NANOCRESCENT SERS PROBE - This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide. | 01-09-2014 |
20140187436 | NANOPLASMONIC MOLECULAR RULER FOR NUCLEASE ACTIVITY AND DNA FOOTPRINTING - This invention provides a nanoplasmonic molecular ruler, which can perform label-free and real-time monitoring of nucleic acid (e.g., DNA) length changes and perform nucleic acid footprinting. In various embodiments the ruler comprises a nucleic acid attached to a nanoparticle, such that changes in the nucleic acid length are detectable using surface plasmon resonance. The nanoplamonic ruler provides a fast and convenient platform for mapping nucleic acid-protein interactions, for nuclease activity monitoring, and for other footprinting related methods. | 07-03-2014 |
20140342427 | PHOTONIC NANOANTENNA MEDIATED GENE CIRCUIT RECONFIGURATION - A selectively addressable optical biomolecular carrier and its method of use for reconfiguring gene circuits are described. One carrier is a plasmon resonant nanoantenna formed from a gold metal nanorod coated with a cationic phospholipid bilayer with an aspect ratio between 2.0 and 8.0 and plasmon resonance wavelength in the near infrared range. Biomolecules such as siRNA adhere to the carrier and are introduced into a cell. The biomolecules are released from the nanoantenna carriers with exposure to light at the plasmon resonance wavelength. The nanoantenna efficiently converts absorbed optical energy to surface localized heat releasing the biomolecules at a time determined by the user. The carrier can be used to modify gene circuits by allowing temporal control over the genes within a selected gene circuit through the optical release of interfering nucleotides. Optical silencing of endogenous genes with siRNA released from nanoantenna carriers was used to illustrate the methods. | 11-20-2014 |