Patent application number | Description | Published |
20100210827 | Preparation Method Of Recombinant Protein By Use Of A Fusion Expression Partner - The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR type peptidyl prolyl cis-trans isomerase). Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component). RpoS (RNA polymerase sigma factor) PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins. | 08-19-2010 |
20110281300 | PREPARATION METHOD OF RECOMBINANT PROTEIN BY USE OF A FUSION EXPRESSION PARTNER - The present invention relates to a preparation method using a fusion expression partner. The method includes preparing a polynucleotide encoding a fusion expression partner selected from the group consisting of SlyD (FKBR-type peptidyl prolyl cis-trans isomerase), Crr (glucose-specific phosphotransferase (PTS) enzyme IIA component), RpoS (RNA polymerase sigma factor), PotD (Spermidine/putrescine-binding periplasmic protein), and RpoA (RNA polymerase alpha subunit), and an expression vector linking a polyDNA fragment of a heterologous protein, preparing a transformant by introducing the expression vector into a host cell, inducing the expression of a recombinant protein by culturing a transformant, and obtaining the expression. In the preparation method of the recombinant protein, the heterologous protein may enhance the water-solubility and folding of the recombinant protein, overcome the limitations about the water-solubility and folding which the conventional fusion expression partners have, and be used widely in the production of pharmaceutical and industrial proteins. | 11-17-2011 |
20120000779 | SURFACE ACOUSTIC WAVE SENSOR DEVICE INCLUDING TARGET BIOMOLECULE ISOLATION COMPONENT - Provided herein is a surface acoustic wave (“SAW”) sensor device including an isolation component of a target biomolecule. A sample containing the target biomolecule is separated by its size using electrophoresis, and sequentially reacts with a SAW sensor. In other words, the device is capable of detecting the target biomolecule by separating biomolecules using electrophoresis, and applying the separated biomolecules to the SAW sensor. | 01-05-2012 |
20120178178 | BIOSENSOR CARTRIDGE - A biosensor cartridge includes a plasma separator, a plasma metering chamber, a mixer, and a biosensor. | 07-12-2012 |
20120208290 | METHOD FOR ENHANCING MASS OF GOLD NANOPARTICLE THROUGH LIGHT-IRRADIATION, METHOD AND SENSOR FOR DETECTING MOLECULAR BINDING USING THE METHOD FOR ENHANCING MASS - Provided are a sensor for detecting molecular binding by increasing the mass of a gold nanoparticle through light-irradiation and a method thereof. In the method, light-irradiation increases the size of gold nanoparticles without using a reducing agent, to enhance the mass. Accordingly, selectivity may be improved, and the sensitivity of detection may be improved due to a change in various properties of a gold nanoparticle. | 08-16-2012 |
20130115632 | HIGHLY SENSITIVE METHOD FOR ASSAYING TROPONIN I - A highly sensitive method of assaying an analyte present in a sample using protamine is provided, as well as an immunoassay reagent and biosensor that reduces non-specific binding by electrostatic interaction of protamine with fibrinogen present in the sample. | 05-09-2013 |
20130344618 | BIOSENSOR USING AGGLOMERATION OF MAGNETIC NANOPARTICLES AND DETECTION METHOD BY THE SAME - A biosensor and method for detecting a target material using an agglomeration of magnetic nanoparticles are provided. | 12-26-2013 |
20140065645 | POLYPEPTIDE LINKER AND METHOD OF ANALYZING TARGET MATERIAL USING THE SAME - A polypeptide linker comprising an antibody-binding region and an enzyme cleavage region, and related compositions, kits, and methods of using same. | 03-06-2014 |
20140162281 | COMPOSITIONS AND KITS FOR SEPARATING CELLS AND METHOD OF SEPARATING CELLS USING THE SAME - Provided are compositions, kits, and methods for separating cells including complexes of at least one type of linker capable of binding to an antibody or antigen binding fragment and a solid phase. | 06-12-2014 |
20140356890 | METHOD OF RE-COLLECTING TARGET MATERIAL - A method of re-collecting a target material using a filter is provided. The method includes reversely flowing a first fluid from a first flow channel towards a second flow channel through a filter unit so that the target material is included in the first fluid that flows through the second flow channel, wherein the filter includes: the first flow channel, the second flow channel that at least in part overlaps the first flow channel and is disposed above the first flow channel, and the filter unit that is disposed on a region where the first flow channel overlaps the second flow channel and where the target material is captured. | 12-04-2014 |
20150031023 | METHOD OF DETECTING NUCLEIC ACIDS CONTAINING A GENETIC VARIATION - Provided are a composition or a kit for detecting a nucleic acid with genetic variation including a first amplification blocking nucleic acid and a second amplification blocking nucleic acid, and a method of detecting a nucleic acid with genetic variation by using the same. Based on the above, a nucleic acid with genetic variation can be detected with high sensitivity and accuracy. | 01-29-2015 |
20150037801 | METHOD OF AMPLIFYING NUCLEIC ACID - Provided is a method of amplifying a nucleic acid of a cell involving applying ultrasonic waves to lyse a cell bound to a solid support to provide a cell lysate comprising a nucleic acid; separating the cell lysate from the solid support; adding a protease to the cell lysate; and amplifying the nucleic acid. | 02-05-2015 |
20150044725 | METHOD OF SEPARATING NUCLEIC ACIDS - A method of separating nucleic acids from cells, the method comprising incubating a sample comprising cells with a solid substrate that binds to the cells, whereby the cells adhere to the solid substrate; suspending the solid substrate adhered to the cells in a lysis composition comprising about 100 mM to about 300 mM of alkaline metal salt, and having a pH of about 6 to about 8; lysing the cells in the lysis composition to obtain a lysed solution; and obtaining the nucleic acids from the lysed solution; as well as related compositions and kits. | 02-12-2015 |
20150064721 | COMPOSITION AND KIT FOR SEPARATING CANCER CELL, AND METHOD OF SEPARATING CANCER CELL BY USING THE COMPOSITION AND KIT - Provided is a composition and a kit for separating a cancer stem cell or a circulating tumor cell, a method of separating a cancer stem cell or a circulating tumor cell in a biological sample, and a method for diagnosing metastatic cancer. | 03-05-2015 |