Kermekchiev
Milko Kermekchiev, St. Louis, MO US
Patent application number | Description | Published |
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20140363819 | COMPOSITION TO OVERCOME INHIBITORS IN PCR AND GROWTH CULTURES - Provided herein are compositions and methods for improving amplification or detection of a target nucleic acid in a sample containing PCR inhibitors, such as polyphenols. One aspect provides an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof. Another aspect provides a method of amplifying a target nucleic acid with an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof. Another aspect provides a method for culturing microorganisms in media containing PVP or casein. | 12-11-2014 |
Milko B. Kermekchiev, University City, MO US
Patent application number | Description | Published |
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20090170060 | USE OF WHOLE BLOOD IN PCR REACTIONS - A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase. | 07-02-2009 |
20110027832 | USE OF TAQ POLYMERASE MUTANT ENZYMES FOR NUCLEIC ACID AMPLIFICATION IN THE PRESENCE OF PCR INHIBITORS - The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil. | 02-03-2011 |
Milko B. Kermekchiev, St. Louis, MO US
Patent application number | Description | Published |
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20130252309 | MUTANT POLYMERASES WITH FAST ELONGATING ACTIVITY - An isolated polypeptide having fast elongating polymerase activity. Also provided are kits containing the isolated polypeptide and isolated polynucleotides encoding the isolated polypeptide. | 09-26-2013 |
20140113299 | INHIBITION-RESISTANT POLYMERASES - Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or constructs encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such. | 04-24-2014 |