Patent application number | Description | Published |
20150259659 | METHOD FOR OBTAINING NATURAL VARIANT OF ENZYME AND SUPER THERMOSTABLE CELLOBIOHYDROLASE - A method for selectively obtaining a natural variant of an enzyme having activity includes (1) a step of detecting an ORF sequence of a protein having enzyme activity from a genome database including base sequences of metagenomic DNA of environmental microbiota; (2) a step of obtaining at least one PCR clone including the ORF sequence having a full length, a partial sequence of the ORF sequence, or a base sequence encoding an amino acid sequence which is formed by deletion, substitution, or addition of at least one amino acid residue in an amino acid sequence encoded by the ORF sequence, by performing PCR cloning on at least one metagenomic DNA of the environmental microbiota by using a primer designed based on the ORF sequence; (3) a step of determining a base sequence and an amino acid sequence which is encoded by the base sequence for each PCR clone obtained in the step (2); and (4) a step of selecting a natural variant of an enzyme having activity by measuring enzyme activity of proteins encoded by each PCR clone obtained in the step (2). | 09-17-2015 |
20150259660 | THERMOSTABLE CELLOBIOHYDROLASE AND AMINO ACID SUBSTITUTED VARIANT THEREOF - A thermostable cellobiohydrolase including a cellobiohydrolase catalytic domain, the cellobiohydrolase catalytic domain including:
| 09-17-2015 |
20160024486 | THERMOSTABLE XYLANASE BELONGING TO GH FAMILY 10 - A thermostable xylanase including a xylanase catalytic domain, the xylanase catalytic domain including:
| 01-28-2016 |
20160032265 | THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 - A thermostable β-xylosidase including a β-xylosidase catalytic domain, the β-xylosidase catalytic domain including:
| 02-04-2016 |
20160060666 | THERMOSTABLE XYLANASE BELONGING TO GH FAMILY 10 - A thermostable xylanase having a xylanase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having xylanase activity at least under conditions of 85° C. and pH 6.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having xylanase activity at least under conditions of 85° C. and pH 6.0. | 03-03-2016 |
20160076015 | THERMOSTABLE BETA-GLUCOSIDASE - A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including:
| 03-17-2016 |
20160076016 | THERMOSTABLE BETA-GLUCOSIDASE - A thermostable β-glucosidase including a β-glucosidase catalytic domain, the β-glucosidase catalytic domain including:
| 03-17-2016 |
20160076017 | THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 - A thermostable β-xylosidase, having a β-xylosidase catalytic domain including: (A) a polypeptide including the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, (B) a polypeptide including an amino acid sequence in which at least one amino acid has been deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0, or (C) a polypeptide including an amino acid sequence having 80% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1, 3 or 5, and having hydrolysis activity against a substrate of p-nitrophenyl-β-D-xylopyranoside at least under conditions of 80° C. and pH 4.0. | 03-17-2016 |
20160108385 | HYPERTHERMOSTABLE ENDOGLUCANASE BELONGING TO GH FAMILY 12 - A hyperthermostable endoglucanase including an endoglucanase catalytic domain, the endoglucanase catalytic domain including:
| 04-21-2016 |