Patent application number | Description | Published |
20130164797 | PROCESS FOR THE IMPROVED SEPARATION OF A HYDROPHOBIC ORGANIC SOLUTION FROM AN AQUEOUS CULTURE MEDIUM - A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof. | 06-27-2013 |
20140141478 | PROCESS FOR REACTING A CARBOXYLIC ACID ESTER - The invention provides a process for reacting a carboxylic acid ester of the formula (I) | 05-22-2014 |
20140178948 | PRODUCTION OF OMEGA-AMINO FATTY ACIDS - The invention provides a whole cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase phosphopantetheinylating the fatty acid reductase, and which in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase expresses a transaminase, characterized in that the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and a method for the conversion of a fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or a monoester thereof to an amine, comprising oxidation of the fatty acid, ω-hydroxy fatty acid, ω-oxo fatty acid or the monoester thereof to an oxidation product by contacting with an alkane hydroxylase and/or alcohol dehydrogenase, contacting the oxidation product with a phosphopantetheinylated fatty acid reductase or a α-dioxygenase to give an aldehyde, and contacting the aldehyde with a transaminase. | 06-26-2014 |
20140256904 | BIOTECHNOLOGICAL SYNTHESIS PROCESS OF OMEGA-FUNCTIONALIZED CARBON ACIDS AND CARBON ACID ESTERS FROM SIMPLE CARBON SOURCES - The subject of the invention is a biotechnological process for the production of ω-functionalized carboxylic acids and of ω-functionalized carboxylate esters from simple carbon sources. | 09-11-2014 |
20150111253 | ENZYMATIC OMEGA-OXIDATION AND OMEGA-AMINATION OF FATTY ACIDS - The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof. | 04-23-2015 |
20150111254 | METHOD FOR AEROBICALLY PRODUCING ALANINE OR A COMPOUND PRODUCED USING ALANINE - The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell. | 04-23-2015 |
20150307906 | PRODUCING AMINES AND DIAMINES FROM A CARBOXYLIC ACID OR DICARBOXYLIC ACID OR A MONOESTER THEREOF - The invention relates to a whole-cell catalyst which expresses a recombinant α-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the α-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an α-dioxygenase and contacting the product with a transaminase. | 10-29-2015 |