| Patent application number | Description | Published |
|---|
| 20110223585 | ASSAY FOR LOCALIZED DETECTION OF ANALYTES - The present invention relates to a method for detecting an analyte in a sample, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, wherein said probes each comprise an analyte-binding moiety and can simultaneously bind to the analyte, and wherein (i) said first proximity probe comprises a nucleic acid moiety attached at one end to the analyte-binding moiety, wherein a circular or circularizable oligonucleotide is hybridized to said nucleic acid moiety before, during or after said contacting step; and (ii) said second proximity probe comprises an enzyme moiety, attached to the analyte-binding moiety, capable of directly or indirectly enabling rolling circle amplification (RCA) of the circular or, when it is circularized, of the circularizable oligonucleotide hybridized to the nucleic acid moiety of the first proximity probe, wherein said RCA is primed by said nucleic acid moiety of said first proximity probe; (b) if necessary, circularizing said oligonucleotide, to produce a circularized template for RCA; (c) subjecting said circular or circularized template to RCA, wherein if the enzyme moiety of the second proximity probe in step (a)(ii) is a DNA polymerase, this step does not utilize a free DNA polymerase; and (d) detecting a product of said RCA. | 09-15-2011 |
| 20130288249 | Dynamic Range Methods - The present invention relates to methods for detecting and quantifying an analyte in a sample, principally in proximity probe assays, and in particular to an improvement in such methods to extend the dynamic range of detection, which is particularly advantageous for the detection and quantification of an analyte where the concentration range of the analyte in said sample is unknown and/or the range is likely to be broad, said method comprising: (i) contacting said sample with at least a pair of proximity probes each comprising a proteinaceous target-binding domain coupled to a nucleic acid domain such that said nucleic acid domains may be allowed to interact directly or indirectly when the proximity probes have bound in proximity to their respective target, said target being either the analyte or a binding partner for the analyte; (ii) further contacting said sample with at least one set of markers which function to extend the dynamic range of detection of the method, wherein said set comprises at least two markers, wherein each marker is a nucleic acid molecule comprising a binding domain and a reporter domain which gives rise to a detectable signal, and each marker: (a) is capable of interacting either with said nucleic acid domains to form a nucleic acid molecule from which a detectable signal is generated, or with a nucleic acid molecule generated by interaction of said domains; (b) cannot interact with said nucleic acid domains simultaneously with another marker in the set; (c) generates a signal that is distinguishable from the signal of another marker in the set; and (d) is present in an amount capable of detecting the analyte at a range of concentrations that differs from the range of concentrations detectable by other markers; (ii) allowing said markers to interact with said nucleic acid domains or said generated nucleic acid molecule; and (iii) detecting said signal. | 10-31-2013 |
| 20140194311 | Multiplexed Proximity Ligation Assay - The present invention provides a method for detecting interactions between or with any two of at least three target substrates, or any two of at least three features of a target substrate, or a combination of interactions and features of target substrates, by a multiplexed proximity ligation assay, said method comprising: a) for each of the at least three target substrates or features, providing a proximity probe comprising a binding moiety with affinity for the feature or binding site on said substrate, and a proximity probe oligonucleotide coupled on the binding moiety; wherein each of the proximity probe oligonucleotide carries a unique tag sequence; b) mixing the proximity probes with a sample, under a condition to allow binding of each proximity probe to its respective binding site or feature on each of said substrates through the binding moiety, c) simultaneous with, or following step b), forming circularized DNA molecules where any two proximity probes bind sufficiently close to each other on the substrate, wherein each of the circularized DNA molecules comprise complementary sequences to the unique tag sequences from the two proximity probes oligo-nucleotides; d) amplifying the circularized DNA; and e) characterizing the amplified DNA. | 07-10-2014 |
| Patent application number | Description | Published |
|---|
| 20100280957 | SYSTEM, METHOD AND DEVICE FOR ENABLING INTERACTION WITH DYNAMIC SECURITY - A code-generating device is for enabling interaction with dynamic security between a user and a transaction service provider. The code-generating device includes at least one information acquisition device and processing circuitry. In at least one embodiment, the processing circuitry is configured to receive, via the at least one information acquisition device, a transaction-specific code generated by the transaction service provider, evaluate the transaction-specific code, perform, based on the evaluation of the transaction-specific code, a transaction-specific sequence of functions, each involving prompting the user to indicate a respective function-related value, resulting in a sequence of function-related values indicated by the user, and determine a transaction-specific response code based on the sequence of function-related values, thereby enabling secure authentication of the transaction. | 11-04-2010 |
| 20110185180 | METHOD AND DEVICE FOR CREATING DIGITAL SIGNATURE - A method is disclosed for creating a digital signature associated with a user having a code-generating device including a data interface, a display device, a user input device and processing circuitry, the digital signature being indicative of at least one signature object including a signature element having been pre-selected for display to the user. In at least one embodiment, the method includes: receiving, through the data interface, signature data from a user communication device, the signature data including reference data indicating a location of the signature object and a corresponding identifier code, uniquely identifying the signature object; acquiring, through the data interface, the signature object including the signature element having been pre-selected for display; determining a candidate identifier code for the signature object including the signature element having been pre-selected for display; displaying, if the candidate identifier code matches the identifier code included in the signature data, information indicative of the signature element having been pre-selected for display using the display device; determining, if user input indicative of approval of the displayed information is received through the user input device, a digital signature based on the signature data using the processing circuitry; and providing the digital signature to the user communication device. | 07-28-2011 |
