Patent application number | Description | Published |
20090011472 | ISOTHERMAL DNA AMPLIFICATION - Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA. | 01-08-2009 |
20090155859 | CONTAMINATION-FREE REAGENTS FOR NUCLEIC ACID AMPLIFICATION - Methods and kits for generating contamination-free reagents and reagent solutions for use in nucleic acid amplification are provided. Methods include processing of polymerase solutions, nucleotide solutions and primer solutions to render contaminating nucleic acid inert. The methods employ the proofreading activity of the polymerase and/or exonucleases to de-contaminate the reagents and reagent solutions. Methods and kits for contamination-free nucleic acid amplification are provided. | 06-18-2009 |
20100055744 | DNA MINI-CIRCLES AND USES THEREOF - Methods and kits for generating circular nucleic acids in a cell-free system, and uses for the generated circular nucleic acids are provided. The methods comprise in vitro amplification of a nucleic acid template comprising a recombination site to produce tandem repeat nucleic acid sequence, and employ a recombination protein to generate the circular nucleic acids from the tandem repeat nucleic acid sequence. | 03-04-2010 |
20120122733 | COMPOSITION, DEVICE AND ASSOCIATED METHOD - The composition includes a first probe and a first initiator bonded to the first probe. The composition further includes a second probe and a second initiator bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte. An associated kit, device, and method are provided. | 05-17-2012 |
20120122735 | COMPOSITION, DEVICE AND ASSOCIATED METHOD - A composition includes a first probe, a first initiator component bonded to the first probe, a second probe, and a second initiator component bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte, and the first initiator component and the second initiator component are capable of forming an initiator when present in proximity to each other and when the first probe and the second probe are bonded to the analyte. An associated kit, device, and method are provided. | 05-17-2012 |
20120196330 | ISOTHERMAL DNA AMPLIFICATION - Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA. | 08-02-2012 |
20130210078 | METHODS AND KITS FOR REDUCING NON-SPECIFIC NUCLEIC ACID AMPLIFICATION - Methods and kits for efficient amplification of nucleic acids are provided. The disclosure generally relates to methods and kits for nucleic acid amplification of target nucleic acids of interest. The methods described herein promote the synthesis of the target nucleic acid (i.e., template nucleic acid) by reducing the production of undesirable primer-dimer structures and chimeric nucleic acid products during the amplification process by using novel modified primers. | 08-15-2013 |
20140004508 | METHOD FOR ISOTHERMAL DNA AMPLIFICATION STARTING FROM AN RNA TEMPLATE | 01-02-2014 |
20140004509 | KIT FOR ISOTHERMAL DNA AMPLIFICATION STARTING FROM AN RNA TEMPLATE | 01-02-2014 |
20150031035 | METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS - Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane. | 01-29-2015 |