Florin
Bradley J. Florin, Fort Collins, CO US
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20120292238 | PRESSURIZED WATER FILTRATION SYSTEM - A piston pressurized water filter activated by the user or external source. The water filter assembly includes a hollow cylindrical water reservoir, a hollow cylindrical piston threaded into the top of the water reservoir, and a water filter threaded into the bottom of the water reservoir. The interior of the water reservoir is sealed by the piston and water filter. When the water reservoir is filled with unfiltered water, the piston can be threaded down into the water reservoir to create sufficient pressure on the unfiltered water and force the water into the water filter. The water filter can include any combination of carbon, ceramic or other filtering material in a block or disk form. Water is forced into the water filter, through the carbon or ceramic filter mass, and into a container that may or may not be coupled to the water filter. | 11-22-2012 |
Bruno Jean-Marie Florin, St. Cyr-Sur-Loire FR
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20100236143 | COCOA SOMATIC EMBRYOGENESIS - A process for the propagation of | 09-23-2010 |
Charles Florin, Exton, PA US
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20090161926 | Semi-automatic Segmentation of Cardiac Ultrasound Images using a Dynamic Model of the Left Ventricle - A method for segmenting a sequence of images includes developing an autoregressive model using training data including segmented images of a same type as the sequence of images. The sequence of images showing a progression of a subject through a cycle is acquired. At least two images from the sequence of images are identified. A region of interest is manually segmented from the identified images. The manually segmented images are parameterized. The autoregressive model is adapted to the parameterized segmented images. The autoregressive model is used to perform segmentation on the region of interest for a plurality of images of the sequence of images. | 06-25-2009 |
Charles Florin, Trenton, NJ US
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20110123095 | Sparse Volume Segmentation for 3D Scans - A computer readable medium is provided embodying instructions executable by a processor to perform a method for sparse volume segmentation for 3D scan of a target. The method including learning prior knowledge, providing volume data comprising the target, selecting a plurality of key contours of the image of the target, building a 3D spare model of the image of the target given the plurality of key contours, segmenting the image of the target given the 3D sparse model, and outputting a segmentation of the image of the target. | 05-26-2011 |
Charles Henri Florin, Trenton, NJ US
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20110200227 | ANALYSIS OF DATA FROM MULTIPLE TIME-POINTS - Described herein is a technology for facilitating analysis of data across multiple time-points. In one implementation, first and second images acquired at respective first and second different time-points are received. In addition, first and second findings associated with the first and second images respectively are also received. The first and second findings are associated with at least one region of interest. A correspondence between the first and second findings may be automatically determined by aligning the first and second findings. A longitudinal analysis result may then be generated by correlating the first and second findings. | 08-18-2011 |
Didier P. Florin, Sunnyvale, CA US
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20120227665 | APPARATUS FOR MONITORING AND CONTROLLING SUBSTRATE TEMPERATURE - A system and methods for heating substrates during high temperature processing is provided. The system uses multiple temperature inputs of the backside of a substrate carrier and known parameters within the processing chamber to estimate the temperature of substrates being processed on the substrate carrier. Temperature readings of the substrate carrier taken from above the processing volume may be used to correct any drift that may occur with respect to temperature readings taken from below the substrate carrier. Temperature readings of heat exchanging fluid flowing through a showerhead assembly may be used to estimate the temperature of the surface of the showerhead, which may be used in the estimation of the temperature of the substrates being processed. The system then uses the estimated temperature to control the amount of power supplied to a plurality of heat sources configured to heat the substrates from below the substrate carrier. | 09-13-2012 |
Lore Florin, Danbury, CT US
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20150140591 | CELL ENGINEERING USING RNAs - The invention concerns the field of cell culture technology. It concerns RNA having a specific sequence, expression vectors encoding said RNA, production host cell lines comprising said RNA, and methods of producing recombinant biopharmaceutical products using engineered host cell with altered levels of said RNAs, such as small non-coding RNAs, preferably microRNAs (miRNAs). The invention also relates to engineered host cells with altered levels in one or more of said RNAs. Those cell lines have improved secretion and/or growth characteristics in comparison to control cell lines. | 05-21-2015 |
Lore Florin, Biberach DE
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20080300207 | PROTEIN PRODUCTION - The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport. | 12-04-2008 |
20090018099 | PROTEIN PRODUCTION - The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport. | 01-15-2009 |
20100021911 | PRODUCTION HOST CELL LINES - The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a DHFR expression cassette. Those cell lines have improved growth characteristics in comparison to DHFR-deficient or DHFR-reduced cell lines such as CHO DG44 and CHO DUKX-B11. The invention especially concerns two cell lines, a representative of each cell line is deposited with the DSMZ under the number DSM ACC2909 (CHOpperĀ® Discovery) and DSM ACC2910 (CHOpperĀ® Standard). The invention further concerns a method of producing proteins using the cells generated by the described method. | 01-28-2010 |
20100086967 | CELL GROWTH - The invention concerns the field of cell culture technology. It concerns a method of improving cell growth, especially the growth of biopharmaceutical producer host cells. The invention further concerns a method of producing proteins using the cells generated by the described method. | 04-08-2010 |
20110124108 | EPIGENETIC ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. | 05-26-2011 |
20110230642 | METHOD FOR INCREASING RECLONING EFFICIENCY - The present invention relates to the field of cell culture technology and relates to methods of replicating/cloning cells, preferably cell lines which are important for the production of biopharmaceuticals. The invention also relates to methods of preparing proteins using cells that have been obtained and replicated by single cell deposition and compositions which make it possible to replicate individual cells. | 09-22-2011 |
20110262967 | USE OF HSA-PRODUCING CELLS - The present invention refers to the area of cell culture technology and relates to methods for multiplying/cloning cells, preferably cell lines, which are important to the production of biopharmaceuticals. The invention further relates to methods for manufacturing proteins and to the use of cells extracted and multiplied through single cell sorting and to media compositions that enable a multiplication of single cells. Through the use of albumin-producing, preferably HSA-producing, cells as feeder cells for the conditioning of medium or as host cells, the recloning efficiency and thereby the quantity of clones obtained can be significantly increased. A combination of these approaches is also possible. Through the use of albumin-producing, preferably HSA-producing, cells, an increase in the recloning efficiency can be achieved in serum-free and/or insulin-free medium as well, and in different cell types. | 10-27-2011 |
20110281301 | THE SECRETORY CAPACITY IN HOST CELLS - The invention concerns the field of protein production and cell culture technology. It describes a method of producing a heterologous protein of interest in a cell comprising a. Increasing the expression or activity of a secretion enhancing gene, and b. Increasing the expression or activity of an anti-apoptotic gene, and c. Effecting the expression of said protein of interest, whereby the secretion enhancing gene is a gene encoding a protein whose expression or activity is induced during one of the following cellular processes: plasma-cell differentiation, unfolded protein response (UPR), endoplasmic reticulum overload response (EOR). | 11-17-2011 |
20130177919 | PROTEIN PRODUCTION - The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport. | 07-11-2013 |
Lore Florin, Wien AT
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20130196430 | PROTEIN PRODUCTION - The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport. | 08-01-2013 |
20130197196 | PROTEIN PRODUCTION - The invention concerns the field of protein production and cell culture technology. CERT is identified as a novel in vivo PKD substrate. Phosphorylation on serine 132 by PKD decreases the affinity of CERT towards its lipid target phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity, identifying PKD as a regulator of lipid homeostasis. The present invention shows that CERT in turn is critical for PKD activation and PKD dependent protein cargo transport to the plasma membrane. The interdependence of PKD and CERT is thus a key to the maintenance of Golgi membrane integrity and secretory transport. | 08-01-2013 |
Lore Florin, Vienna AT
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20130210074 | EPIGENETIC ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through reducing expression of NoCR proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. | 08-15-2013 |
Lore Florin, Biberach An Der Riss DE
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20120100553 | CHO/CERT CELL LINES - The invention concerns the field of cell culture technology. The invention describes production host cell lines comprising vector constructs comprising a CERT S132 A expression cassette. Those cell lines have improved growth characteristics and high CERT S132A expression levels. The invention especially concerns two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM AC-C2990 (CHO/CERT 2.41). The invention further concerns a method of generating such preferred production host cells and a method of producing proteins using the two cell lines deposited with the DSMZ under the number DSM ACC2989 (CHO/CERT 2.20) and DSM ACC2990 (CHO/CERT 2.41). | 04-26-2012 |
20120190065 | COMBINATORIAL ENGINEERING - The invention concerns the field of cell culture technology. It concerns production host cell lines with increased expression of ribosomal RNA (rRNA) achieved through introduction of nucleic acids encoding UBF or reducing expression of NoRC proteins, especially of TIP-5. Those cell lines have improved secretion and growth characteristics in comparison to control cell lines. The invention further concerns a method of producing proteins using the cells generated by the described method. | 07-26-2012 |
20120258496 | PRODUCTION OF LOW FUCOSE ANTIBODIES IN H4-II-E RAT CELLS - The invention concerns the field of cell culture technology. It specifically concerns a rat hepatoma cell, preferably a H4-II-E rat hepatoma cell, carrying a DNA encoding an antibody or Fc-fusion protein and having low fucosylation activity for adding fucose to glycosidic structures such as biantennary glycans, e.g. N-acetylglucosamine. The invention furthermore concerns a method for producing low fucose glycoproteins especially antibodies or Fc-fusion proteins in rat hepatoma cells, preferably in H4-II-E rat hepatoma cells. It further concerns the identification and generation of new host cell lines which are capable of synthetizing glycoproteins with beneficial properties, improving the therapeutic efficacy and/or serum half-life of the product compared to products from commonly used host cell lines. | 10-11-2012 |
Lore Florin, Ingelheim DE
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20090247609 | SM-PROTEIN BASED SECRETION ENGINEERING - The present invention concerns the field of cell culture technology. It describes a novel method for enhancing the secretory transport of proteins in eukaryotic cells by heterologous expression of Munc18c, Sly1 or other members of the SM protein family. This method is particularly useful for the generation of optimized host cell systems with enhanced production capacity for the expression and manufacture of recombinant protein products. | 10-01-2009 |
Peter Florin, Berkeley, CA US
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20150023789 | Wind Turbine Power Augmentation - Wind power generator. The generator includes a wind turbine rotating in an aerodynamically contoured duct and capable of generating increased power by increasing the wind flow at the turbine. A wind turbine is located at the entrance to a diverging duct. The duct expands in the wind direction following the wind turbine section, increasing the flow. A porous disk is located at the trailing edge of the aerodynamically contoured duct and this disk reduces die pressure at the trailing edge of the duct thereby sharply accelerating the flow through the duct and increasing die power output of die wind turbine. The porosity of the disk can be geometrically modified such dial the flow field changes to respond to changing wind conditions. | 01-22-2015 |
Sandro Florin, Corsier-Sur-Vevey CH
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20120145011 | USER-FRIENDLY START-UP MODE OF A BEVERAGE PREPARATION MACHINE - An electric beverage preparation machine has: an arrangement ( | 06-14-2012 |
Timothy Henri Jeremy Florin, Buranda AU
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20130005749 | NOVEL FORMULATION AND TREATMENT METHODS - The invention relates to extended release compositions and formulations comprising 6-thioguanine (6-TG), and methods for treating diseases or conditions responsive to 6-TG. | 01-03-2013 |
Wilhelm Florin, Duisburg DE
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20130061685 | METHOD FOR OPERATION OF SEVERAL ADJACENT MAGNETIC-INDUCTIVE FLOW METERS - A method for operation of several adjacent magnetic-inductive flow meters, each of which has a measurement tube through which an electrically conductive medium flows, a magnetic field generating apparatus for permeating the medium with a magnetic field having a component perpendicular to the longitudinal axis of the measurement tube, and a measurement apparatus for measuring the voltage induced into the medium and for determining the flow rate from the induced voltage. Each flow meter has a control apparatus. In order to prevent variations of mutual influences on the flow rate measurements by the magnetic fields of adjacent flow meters, measured flow rate values from individual measurement processes and synchronous measurement processes are used to determine the mutual influences on the flow rate measurements, and influences that are free of variations are compensated by the magnetic fields of the adjacent flow meters being synchronized. | 03-14-2013 |