Patent application number | Description | Published |
20120100549 | TARGETED GENOME AMPLIFICATION METHODS - The methods disclosed herein relate to methods and compositions for amplifying nucleic acid sequences, more specifically, from nucleic acid sequences of pathogens by targeted genome amplification. In certain embodiments, multiple primer pairs are employed that flank a target region and polymerization is conducted with a strand displacing enzyme. | 04-26-2012 |
20120171726 | RAPID WHOLE GENOME AMPLIFICATION - The present invention provides compositions and methods for rapidly amplifying target nucleic acid (e.g., using whole genome amplification) that allows small amounts of starting nucleic acid to be employed. In certain embodiments, the methods employ compositions that comprise: phi29 polymerase, exo- Klenow polymerase and/or Klenow polymerase, dNTPs, primers, and a buffering agent. In some embodiments, the target nucleic acid is amplified at a rate that would result in at least 1000-fold amplification in thirty minutes. | 07-05-2012 |
20120172258 | NUCLEIC ACID SAMPLE PREPARATION METHODS AND COMPOSITIONS - The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification. | 07-05-2012 |
20160051959 | NUCLEIC ACID SAMPLE PREPARATION METHODS AND COMPOSITIONS - The present invention provides compositions and methods for preparing a nucleic acid library in a multi-purpose buffer (e.g., employing whole genome amplification), where nucleic acid purification is not required between or during steps. In certain embodiments, small amounts of starting nucleic acid (e.g., genomic DNA) are employed and the steps are accomplished in a single container. In some embodiments, the nucleic acid library is subjected to sequencing methodologies or rolling circle amplification. | 02-25-2016 |
Patent application number | Description | Published |
20130337452 | Methods For Rapid Forensic Analysis Of Mitochondrial DNA - The present invention provides methods for rapid forensic analysis of mitochondrial DNA by amplification of a segment of mitochondrial DNA containing restriction sites, digesting the mitochondrial DNA segments with restriction enzymes, determining the molecular masses of the restriction fragments and comparing the molecular masses with the molecular masses of theoretical restriction digests of known mitochondrial DNA sequences stored in a database. | 12-19-2013 |
20140275216 | ALTERATION OF NEURONAL GENE EXPRESSION BY SYNTHETIC piRNAs AND BY ALTERATION OF piRNA FUNCTION - Provided herein are compositions and methods for the alteration of neuronal methylation by synthetic piRNAs or by alteration of piRNA function. Such alterations find use in the regulation and control of neural gene expression and concomitant neural functions. Further provided herein are systems and methods for the identification of target sites for regulation by piRNAs. | 09-18-2014 |
20140323319 | SYSTEMS AND METHODS FOR ISOLATING NUCLEIC ACIDS - The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for isolating nucleic acids from aqueous samples (e.g., blood or urine). | 10-30-2014 |
20140349858 | AMPLIFICATION OF A SEQUENCE FROM A RIBONUCLEIC ACID - The present invention relates to methods and compositions for tagging, amplifying, purifying, and or characterizing of ribonucleic acid (RNA) in a sample. In particular, methods are provided for preparing RNA from a sample for subsequent analysis. | 11-27-2014 |
20150024398 | ANALYSIS OF GENETIC BIOMARKERS FOR FORENSIC ANALYSIS AND FINGERPRINTING - The present invention relates generally to methods of determining base compositions for PCR products (e.g., RT PCR products, (rt) RT-PCR products, etc.). In particular, the present invention provides base-composition determination of PCR products containing up to five different nucleobases (e.g., A, C, G, T, U) and/or significant levels of non-templated adenylation. | 01-22-2015 |
20150031038 | SAMPLE PREPARATION METHODS - The present invention provides whole blood nucleic acid extraction methods, compositions, and kits, as well as nested isothermal amplification methods, compositions, and kits. In certain embodiments, these methods are applied to detecting Lyme disease, including in patients without classic erythema migrans skin lesions. | 01-29-2015 |
20150031117 | METHODS FOR RAPID FORENSIC ANALYSIS OF MITOCHONDRIAL DNA AND CHARACTERIZATION OF MITOCHONDRIAL DNA HETEROPLASMY - The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases. | 01-29-2015 |
20150111762 | DNA SEQUENCING - Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to methods, compositions, and systems for sequencing a nucleic acid using one or more labels and signal amplitude to distinguish bases. | 04-23-2015 |
20150111763 | DNA SEQUENCING - Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to methods, compositions, and systems for sequencing a nucleic acid using two or more labels and signal ratios to distinguish bases. | 04-23-2015 |
20150184231 | BIOAGENT DETECTION OLIGONUCLEOTIDES - The present invention compositions, methods and systems to identify, detect, and/or quantify bacterial DNA in the presence of contaminating non-bacterial DNA. In particular, the present invention provides oligonucleotides configured to detect a relatively small amount of bacterial DNA in the presence of an overwhelmingly large amount of contaminating human DNA. | 07-02-2015 |
20150184238 | DNA SEQUENCING - Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to methods, compositions, systems, and kits for sequencing a nucleic acid using a degenerate two-base code. Particular embodiments provide: 1) that the two-base degenerate code relates a first element to a base comprising adenine (A) or guanine (G) and a second element to a base comprising cytosine (C) or thymine (T); 2) that the two-base degenerate code relates a first element to a base comprising A or C and a second element to a base comprising G or T; and 3) that the two-base degenerate code relates a first element to a base comprising G or C and a second element to a base comprising A or T. | 07-02-2015 |
20150329855 | AMPLIFICATION PRIMERS AND METHODS - The present invention provides methods, compositions, and kits for performing amplification (e.g., whole genome amplification) employing primers that have a 5′ restriction site, a 3′ random sequence (e.g., a random hexamer), and an identifiable barcode sequence. In certain embodiments, the amplification generates individual amplified sequenced that are ligated together to form concatamers containing at least two amplified sequences (e.g., not contiguous on the original target sequence) that are separated by the barcode sequences. In particular embodiments, a plurality of the concatamers are sequenced and aligned with an alignment algorithm that uses the barcode sequences to identify artificial junctions between amplified sequences. | 11-19-2015 |
20150346188 | STABILIZED NANOPORE AND MICROPORE STRUCTURES AND METHODS FOR MAKING AND USING THE SAME - The invention relates generally to nanopore and micropore structures, methods for making the structures and use of the structures. In particular, the invention provides nanopore structures (e.g., containing lipid bilayers) in combination with one or more osmoprotective compounds and methods of making and using the same. Compositions and methods of the invention fmd use in a wide range of applications including molecular biology and medical science. | 12-03-2015 |