Patent application number | Description | Published |
20080308422 | Protein Sample Preparation - In one aspect, the invention provides methods for protein sample preparation for electrophoretic separation. The method comprises a step of providing a protein sample in solution, adding a chaotrope to the protein sample, adding a surfactant to the protein sample, wherein the final concentration of the surfactant in the solution is less than critical micelle concentration of the surfactant. In another aspect, the invention also provides methods of electrophoretic separation of protein samples that includes the protein sample preparation method as described herein. In yet another aspect, the invention provides a loading buffer solution composition for protein sample preparation that includes a surfactant in solution at a final concentration that is less than critical micelle concentration of the surfactant, and; a chaotrope. | 12-18-2008 |
20090071832 | MICROFLUIDIC DEVICE WITH VERTICAL INJECTION APERTURE - A microfluidic device with a vertical injection aperture is provided. The microfluidic device comprises a separation channel, an injection aperture disposed adjacent to and in fluid communication with the separation channel. The microfluidic device further comprises a semi-permeable filter disposed adjacent to the injection aperture, wherein the filter is configured to preconcentrate a sample in the injection aperture to form a preconcentrated sample plug during an injection operation, and wherein the sample plug flows downwardly from the injection aperture to the separation channel during an electrophoresis operation. | 03-19-2009 |
20090166201 | INJECTION METHOD FOR MICROFLUIDIC CHIPS - A microfluidic chip comprising a separation channel configured to receive a sieving matrix and a buffer and an injection channel in fluid communication with the separation channel. The injection channel is configured to receive a sample using a capillary force and a portion of the sample injects into the separation channel electro-kinetic force exerted on the sample. | 07-02-2009 |
20090166202 | INJECTION METHOD FOR MICROFLUIDIC CHIPS - A microchip for electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well. The injection channel and the separation channel form a ‘T’ junction. The microchip comprises a first electrode disposed at a first end of the separation channel, a second electrode disposed in front of the ‘T’ junction and adjacent to the first electrode, a third electrode disposed at a first end of the injection channel and a fourth electrode disposed at a second end of the separation channel. A portion of the sample is injected and separated into an area between the ‘T’ junction and the fourth electrode. | 07-02-2009 |
20090166203 | INJECTION METHOD FOR MICROFLUIDIC CHIPS - A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel. | 07-02-2009 |
20100032582 | FLUORESCENCE DETECTION SYSTEM AND METHOD - A fluorescence detection system comprises a light source configured to produce an excitation light, an optical lens and a fiber bundle. The optical lens is configured to focus the excitation light to a sample to emit fluorescence and to collect the fluorescence. The fiber bundle probe comprises a transmitting fiber configured to transmit the excitation light to the optical lens, and a first receiving fiber configured to deliver the collected fluorescence. The fluorescence detection system further comprises a first detector configured to detect the fluorescence delivered by the receiving fiber to generate a response signal, and a processing unit configured to determine information about the samples by analyzing the response signal. Additionally, a fluorescence detection method is also presented. | 02-11-2010 |
20100096267 | SYSTEM AND METHOD FOR PERFORMING MICROFLUIDIC MANIPULATION - Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir. | 04-22-2010 |
20100101951 | ELECTROPHORESIS SYSTEM AND METHOD - Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir. | 04-29-2010 |
20100155244 | INJECTION METHOD FOR MICROFLUIDIC CHIPS - Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels. | 06-24-2010 |
20110129863 | METHODS AND SYSTEMS FOR PROCESSING SAMPLES ON POROUS SUBSTRATES - Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate. | 06-02-2011 |
20110132111 | METHODS AND SYSTEMS TO PREVENT PUNCH LOSS DURING AUTOMATED SAMPLE PROCESSING - Automated methods and systems for punching out pieces of a porous substrate for biological samples comprising: loading the porous substrate onto a support comprising a die and an opening; moving a receptacle support in at least a z-direction to position a receptacle relative to the support so that an opening in the receptacle is aligned and substantially flush with the opening in the support; actuating a punching head so that the punching head passes through the die, thereby punching a piece out of the porous substrate; and actuating an ejector pin to eject the punched piece from the porous substrate support and into the receptacle aligned with the opening in the support. | 06-09-2011 |
20120152743 | METHOD FOR ELECTROELUTING GENETIC MATERIAL FROM DRIED SAMPLES - In accordance with the present disclosure, a method for extracting genetic material from a biological sample stored on a solid medium is provided. The method includes obtaining the solid medium, wherein the biological sample is applied on the solid medium, and the solid medium includes chemicals that lysed the biological sample and preserved the genetic material. The method also includes electroeluting the genetic material directly from the solid medium to a subsequent medium. | 06-21-2012 |
20140039177 | METHODS OF ISOLATING NUCLEIC ACIDS UNDER REDUCED DEGRADATION CONDITION - A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb. | 02-06-2014 |
20140248714 | METHODS AND SYSTEMS FOR PROCESSING SAMPLES ON POROUS SUBSTRATES - Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate. | 09-04-2014 |
20150031035 | METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS - Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane. | 01-29-2015 |
20150050654 | DETECTION OF NUCLEIC ACID AMPLIFICATION IN A POROUS SUBSTRATE - The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated. | 02-19-2015 |