Patent application number | Description | Published |
20090029477 | Ultra High-Throughput Opti-Nanopore DNA Readout Platform - Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises ( | 01-29-2009 |
20110053284 | CHEMICAL FUNCTIONALIZATION OF SOLID-STATE NANOPORES AND NANOPORE ARRAYS AND APPLICATIONS THEREOF - Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores −10 nm diameter can be reproducibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing. | 03-03-2011 |
20110257043 | ULTRA HIGH-THROUGHPUT OPTI-NANOPORE DNA READOUT PLATFORM - Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises (1) the electrically controlled unzipping of DNA (or RNA) double strands, and (2) the readout of the molecule's identity (or code) using one or more molecule signal detection. | 10-20-2011 |
20120040869 | SEQUENCE PRESERVED DNA CONVERSION - Described herein are inexpensive high throughput methods to convert a target single stranded DNA (ssDNA) such that each nucleotide (or base) adenine (A), thymine (T), guanine (G) and cytosine (C) is converted to a pre-determined oligonucleotide code, with the sequential order preserved in the converted ssDNA, or RNA. The method does not require the use of DNA polymerases during the cycles and involves the use of an oligonucleotide probe library with repeated cycles of ligation and cleavage. At each cycle, one or more nucleotides on one end (e.g., either the 5′ end or the 3′ end) of a target, e.g., ssDNA, are cleaved and then ligated with the corresponding oligonucleotide code at the other end of the target ssDNA. | 02-16-2012 |
20120135410 | METHOD FOR IMAGING ON THIN SOLID-STATE INTERFACE BETWEEN TWO FLUIDS - Described herein is a fluid cell for an optical microscopy tool having a solid state membrane having a first side and a second, opposing side; a first fluid chamber comprising a first fluid having a first refractive index located on the first side of the membrane; and, a second fluid chamber comprising a second fluid having a second refractive index located on the second side of the membrane, the second refractive index being different than the first refractive index. Also described herein is a method for imaging a single biomolecule, the method including generating a field of evanescent illumination at a solid state membrane between a first fluid and a second fluid having different refractive indexes; and detecting light emitted by optical detectors linked to the single biomolecules at the solid state membrane. | 05-31-2012 |
20120199482 | MANUFACTURE OF NANOPARTICLES USING NANOPORES AND VOLTAGE-DRIVEN ELECTROLYTE FLOW - Disclosed are methods of manufacturing nanoparticles such as quantum dots at desired nanopore locations in a membrane. The methods disclosed use voltage-driven electrolyte flow to drive the nanoparticle formation. | 08-09-2012 |
20120276530 | LABEL-FREE SENSING OF PNA-DNA COMPLEXES USING NANOPORES - Embodiments disclosed herein relate to a method of detecting specific DNA sequences and the application of this method in the detection of pathogens, viruses, drug-resistant pathogens, genomic variations associated with disease/disorder susceptibility etc. based on specific signature sequences unique to the pathogens, viruses, drug-resistant pathogens or genomic variations. The method can also be used to distinguish a pool of same-sized dsDNA on the basis of sequence differences. The method uses non-optically labeled bis-PNA and/or gamma-PNA probes to tag specific target sequences for identification by solid-state nanopores. | 11-01-2012 |
20120316075 | SEQUENCE PRESERVED DNA CONVERSION FOR OPTICAL NANOPORE SEQUENCING - The present invention relates to a method for conversion of a target nucleic acid molecule according to a predetermined nucleotide code into a converted nucleic acid molecule. The converted nucleic acid molecule has utility for determining the nucleotide sequence of the target nucleic acid molecule, for example, using a nanopore. | 12-13-2012 |
20130203610 | Tools and Method for Nanopores Unzipping-Dependent Nucleic Acid Sequencing - Provided herein is a library that comprises a plurality of molecular beacons (MBs), each MB having a detectable label, a detectable label blocker and a modifier group. The library is used in conjunction with nanopore unzipping-dependent sequencing of nucleic acids. | 08-08-2013 |
20130256118 | Use of Nanopore Arrays For Multiplex Sequencing of Nucleic Acids - Described are techniques for optical detection of single molecule signals from a nanopore array for analysis of nucleic acid sequences. These techniques are useful for rapid multiplexed DNA sequencing. | 10-03-2013 |