53rd week of 2009 patent applcation highlights part 51 |
Patent application number | Title | Published |
20090325180 | Drug Screening using Islet Cells and Islet Cell Progenitors from Human Embryonic Stem Cells - This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High quality populations of islet cells can be produced in commercial quantities for use in research, drug screening, or regenerative medicine. | 2009-12-31 |
20090325181 | METHODS AND COMPOSITIONS FOR THE SPECIFIC INHIBITION OF GENE EXPRESSION BY DOUBLE-STRANDED RNA - The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery. | 2009-12-31 |
20090325182 | METHODS FOR DETECTING A CYCLOPHILIN B SNP ASSOCIATED WITH HERDA - This invention provides compositions and methods for identification of carriers of Hereditary Equine Regional Dermal Asthenia (HERDA) in equine species. In particular, this invention identifies a single nucleotide polymorphorism (SNP) in cyclophlin B that can be used to identify carriers of HERDA and individuals affected by HERDA. | 2009-12-31 |
20090325183 | SEQUENCING METHODS - The present teachings provide methods and compositions for sequencing one or more target nucleic acids. High levels of multiplexing are provided by the use of an emulsion PCR comprising primer-immobilized beads. The resulting reaction products can be sequenced by any of a variety of mobility-dependent analytical techniques, such as mass spectrometry. In some embodiments, a first collection of amplification products on a first collection of beads are transferred to a second collection of beads. In some embodiments, a first collection of amplification products on a first collection of beads is amplified in a rolling circle amplification reaction. The present teachings also provide compositions, kits, and devices for performing and sequencing the products of the emulsion amplification reactions as described herein. | 2009-12-31 |
20090325184 | Compositions and Methods for Clonal Amplification and Analysis of Polynucleotides - Compositions and methods of use are disclosed for clonally amplifying and analyzing one or more polynucleotides. | 2009-12-31 |
20090325185 | Method of analyzing expression of gene - A cDNA is prepared from mRNA. The prepared cDNA is subjected to incision with two different types of restriction enzymes. The sequence of a portion of each of the fragments obtained as a result of treatment is determined, and the fragments are classified on the basis of the determined sequence by using a primer set. At the same time, the fragments are amplified in a manner that the relative expression magnitude thereof continues to be reflected therein. When the fragments have been classified into a predetermined number of fractions, the respective fractions are subjected to electrophoresis, and a gene expression profile is produced from the results. | 2009-12-31 |
20090325186 | Method for detecting analytes in a sample - The present invention relates to a method for detecting analytes in a sample comprising the steps of
| 2009-12-31 |
20090325187 | Immunomodulation - Medical use of ILT6 for modulating the immune response, as well as to pharmaceutical compositions containing ILT6. | 2009-12-31 |
20090325188 | Method for Detecting IL-16 Activity and Modulation of IL-16 Activity Based on Rantes Proxy Levels - Methods for detecting IL-16 biological activity, detecting modulation of IL-16 biological activity, and diagnosing the presence of or susceptibility of a subject to an IL-16-related disorder involve measuring and comparing the levels of RANTES proxy produced by eukaryotic cells, such as CD4+ and CD9+ cell lines, peripheral blood mononuclear cells, HuT-78 cells, and/or THP-1 cells. | 2009-12-31 |
20090325189 | Tyrosine phosphorylation sites - The invention discloses 443 novel phosphorylation sites identified in leukemia, peptides (including AQUA peptides) comprising a phosphorylation site of the invention, antibodies specifically bind to a novel phosphorylation site of the invention, and diagnostic and therapeutic uses of the above. | 2009-12-31 |
20090325190 | METHOD FOR QUANTITATIVE DETECTION OF DIABETES RELATED IMMUNOLOGICAL MARKERS - This invention discloses using SPR technology to simultaneously and quantitatively measure the concentrations of diabetes related immunological makers in a serum sample, which can be used to diagnose and/or early diagnose diabetes as well as to predict the onset risk of diabetes in first-degree relatives. It also discloses an efficient formula to make a mixed SAM that can greatly enhance the immobilization ability of the metal surface in SPR based techniques, which is good for the immobilization of relevant antigens and antibodies used for the detection of respective diabetes related immunological makers in a serum sample. | 2009-12-31 |
20090325191 | Method for the diagnosis of pathological conditions in animals - A method for diagnosing a disease or pathological condition in a non-human animal. An ion mobility spectrometry measurement (IMS) or a differential mobility spectrometry (DMS) is carried out on a body sample from the animal to determine an amount of ions formed by at least two biogenic amines contained in the sample. A ratio is calculated of the amounts of ions formed by the different biogenic amines in the sample, wherein the ratio is indicative of the presence or absence of the disease or pathological condition. | 2009-12-31 |
20090325192 | Rapid particle detection assay - The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4 | 2009-12-31 |
20090325193 | Diagnostic Test For The Detection Of A Molecule Or Drug In Whole Blood - The invention provides methods of preparing a test sample for use in an assay for detecting an analyte bound by an intracellular ligand. The methods typically involve contacting the test sample with an assay reagent comprising: a lysis reagent; and a protease that has proteolytic activity for said intracellular ligand; to form a mixture compatible for use in an immunoassay without subsequent extraction steps. Other aspects of the invention include related immunoassays and test kits. | 2009-12-31 |
20090325194 | USE OF SPLA2 ACTIVITY FOR THE DIAGNOSIS OF A CARDIOVASCULAR EVENT - A method for determining an increased risk of mortality or of a cardiac and/or vascular event in a patient, includes:—determining the sPLA2 activity of the patient as a first risk marker—determining at least the value of a second risk marker chosen among CRP level, IgM IC of apo B 100 level or IgM MDA-LDL level—determining the ratio (odds ratio) between the value of the sPLA2 activity and the value of the second risk marker and comparing it to a predetermined odds ratio, the odds ratio compared to the predetermined odds ratio being indicative of an increased risk of mortality or of a cardiac and/or vascular event. A new micro method adaptation for automated fluorimetric measurement of serum secretory phospholipase A2 is also disclosed. | 2009-12-31 |
20090325195 | POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PRO-BNP - A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP. | 2009-12-31 |
20090325196 | LEVELS OF BCMA PROTEIN EXPRESSION ON B CELLS AND USE IN DIAGNOSTIC METHODS - The present invention provides a method of measuring the levels of BCMA in a biological sample, specifically upon the B cell surface. The diagnostic assays are useful in predicting an individual's likelihood of developing or currently suffering from an autoimmune disease, such as SLE, and for methods for treating an individual clinically diagnosed with an autoimmune disease. This diagnostic test serves to predict a patient's likelihood to respond to a specific drug treatment, in particular treatment with BLyS antagonists, either singly or in combination with other immune suppressive drugs | 2009-12-31 |
20090325197 | ASSAY FOR IMMUNOSUPPRESSANT DRUGS - The invention provides immunoassays for immunosuppressant drugs, wherein the assay is carried out under high salt conditions to achieve improved sensitivity. The invention also provides kits that are useful for performing the methods of the invention. | 2009-12-31 |
20090325198 | Non-Denaturing Lysis Reagent For Use With Capture-In-Solution Immunoassay - The invention provides a lysis reagent and method for preparing a test sample for use in an assay, wherein the method yields a homogeneous lysis mixture suitable for use in automated pipetting systems without the need for a centrifugation step. The lysis reagent includes a glycol and non-specific animal immunoglobulins. Other aspects of the invention include related immunoassays and test kits. | 2009-12-31 |
20090325199 | NANOSTRUCTURES FOR POLARIZED IMAGING AND RECEPTOR/LIGAN QUANTIZATION: BREAKING THE DIFFRACTION LIMIT FOR IMAGING - The present invention relates to affinity biosensing using polarization of light scattering of aggregated noble metallic nanostructures to determine concentration of an analyte in a test sample. This new sensing system utilizes the changes in polarized plasmonic scattering from nanostructures as the nanostructures aggregate due to binding of the analyte to a binding partner attached to the surface of the metallic nanostructure. | 2009-12-31 |
20090325200 | POLYPYRIMIDINE-TRACT BINDING PROTEIN (PTB) AS A BIOMARKER AND TARGET FOR THE DIAGNOSIS AND TREATMENT OF CANCER - The present invention provides a method of diagnosing cancer in a mammalian subject comprising measuring the level of polypyrimidine-tract binding protein (PTB) in a tissue sample obtained from the subject. An elevated level of PTB in the sample comparing to a normal control is indicative the subject is positively diagnosed with cancer. The present invention furthers provides a method for inhibiting proliferation of a mammalian cancer cell. The method comprises suppressing the level of polypyrimidine-tract binding protein (PTB) in the cell. | 2009-12-31 |
20090325201 | Biomarkers for Detection and Diagnosis of Head and Neck Squamous Cell Carcinoma - Novel, sensitive and specific markers and methods for diagnostics and monitoring of head and neck squamous cell carcinoma (HNSCC) are provided. Kits and methods for the use of hyaluronic acid, hyaluronidase and CD44 to diagnose HNSCC are described. | 2009-12-31 |
20090325202 | Methods for Developing and Assessing Therapeutic Agents - Assays are provided that can effectively assess tumor response to one or more therapeutic agents. Preferred assays of the invention include assessment of posttranslation modification and expression of target proteins. | 2009-12-31 |
20090325203 | Assay For Tissue Factor And Factor Vlla - The invention provides assays for detecting and quantitating tissue factor and factor VIIa in simple and complex biological systems. The assays are performed by detecting and/or measuring the tissue factor cofactor activity and factor VIIa enzymatic activity using aminonaphthalene-based fluorogenic substrates. | 2009-12-31 |
20090325204 | COMPOUNDS AND METHODS FOR DIAGNOSIS AND TREATMENT OF CHAGAS DISEASE - Compounds and methods are provided herein that provide for diagnosis and treatment of Chagas disease. | 2009-12-31 |
20090325205 | METHOD FOR MEASURING ANALYSIS OBJECT, BIOSENSOR AND MEASURING DEVICE - A biosensor | 2009-12-31 |
20090325206 | Method for assaying the activity of lysosomal enzymes - A method, and associated kit, for assaying the activity of lysosomal enzymes present in dried bodily fluids and cell tissue samples, such as α-L-iduronidase, β-D-galactosidase, β-D-glucosidase, chitotriosidase, total α-D-galactosidase and α-D-galactosidase A, hexosaminidase A and B, α-D-mannosidase, β-D-mannosidase, α-L-fucosidase, N-acetyl-α-galactosaminidase, arylsulfatases, sphingomyelinase, β-galactocerebrosidase, iduronate- | 2009-12-31 |
20090325207 | NOVEL PHOSPHODIESTERASE AND GENE THEREOF - The present invention is to provide a novel phosphodiesterase and a gene thereof, specifically, Type 11 phosphodiesterase (PDE11) and a gene thereof, more specifically, a phosphodiesterase selected from (A) a protein having an amino acid sequenced shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: 39, and (B) a protein having an amino acid sequence shown by SEQ.ID.NO: 2, SEQ.ID.NO: 4, SEQ.ID.NO: 6 or SEQ.ID.NO: in which one or several amino acids are deleted, substituted or added, and having an activity of hydrolyzing a cyclic nucleotide, and a gene thereof, and a method of characterizing, identifying and selecting a phosphodiesterase inhibitor by using the same. | 2009-12-31 |
20090325208 | Biosynthesis of Salinosporamide A and Analogs and Methods Thereof - The present invention relates to a sahnosporamide A composition and methods of making salinosporamide A and analogs thereof. The present invention also relates to methods of identifying 2OS proteasome inhibiting agents. | 2009-12-31 |
20090325209 | METHOD AND REAGENT FOR MEASURING NITROREDUCTASE ENZYME ACTIVITY - Disclosed are nitro-substituted squaraine reporter dyes and methods using such dyes for detecting nitroreductase enzyme activity and nitroreductase gene expression in cellular assays. The dyes are of the structure: | 2009-12-31 |
20090325210 | Counting Bacteria and Determining Their Susceptibility to Antibiotics - A method for detecting and counting particles suspended in fluids, such as bacteria suspended in urine, utilizing dynamic features of the suspended particles and employing light scattering measurements. The disclosed method is suitable for determining the susceptibility of bacteria to antibiotics. A cuvette for detecting bacteria in fluids, which is especially suited for the light scattering measurements, is provided. | 2009-12-31 |
20090325211 | System and method for dual-detection of a cellular response - A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor. | 2009-12-31 |
20090325212 | DATA STANDARD FOR BIOMATERIALS - Provided are systems and/or methods that facilitate sensing, detecting, logging, or treatment of a condition or need of a living body using a genetically engineered organism and a data standard. | 2009-12-31 |
20090325213 | DIAGNOSTIC AND THERAPEUTIC APPLICATION OF CTL AND NK FUNCTIONALLY SELECTED CELLS - The invention proposes a novel therapeutic and diagnostic methodology based on the use of effector cells (for example CTL and NK cells) selected for being able to induce lysis in target cells. In particular, the invention teaches how to select strains of effector cells of the immune system according to their lytic properties. It also teaches how diagnostic procedures for checking the activity of therapeutic vaccines can be improved with respect to what shown. Finally, it shows how cell therapies used at present can be improved by using this approach. | 2009-12-31 |
20090325214 | NUCLEAR MATRIX PROTEIN ALTERATIONS ASSOCIATED WITH COLON CANCER AND THEIR USE AS MARKERS FOR COLORECTAL CANCER - CCSA-2 levels in serum samples from individuals suffering from colorectal cancer can be used gauging the progression of a colorectal cancer condition. In particular, the progression of a colorectal cancer condition is gauged by comparing the level of serum CCSA-2 from individuals having colorectal cancer to predetermined CCSA-2 levels that correlate to the state of advancement of a cancer condition. Additionally, serum CCSA-2 levels can be used by a clinician to gauge the efficacy of a colorectal cancer treatment. | 2009-12-31 |
20090325215 | CELLOMICS SYSTEM - In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip. | 2009-12-31 |
20090325216 | Process for the Preparation of Multicellular Spheroids - The invention pertains to a process for the preparation of multicellular spheroids from a suspension of single cells, wherein the cells are directly derived from a biological tissue and/or from cell-containing bodily fluid. The invention is further directed to the multicellular spheroids obtained by the process according to the invention as well as to the use of the spheroids for diagnostic, screening and therapeutic purposes. | 2009-12-31 |
20090325217 | METHOD AND APPARATUS FOR SORTING CELLS - A method, apparatus, and system for a sorting flow cytometer include an objective lens having an optical axis coaxially aligned with the flow path at the focal point. A controllable energy source selectively alters an analyte according to a determination of whether the analyte is in a desired sub-population. In various embodiments, one or both of the emission from the controllable energy source and/or the emission from an illumination energy source passes through the objective lens. In some embodiments in which the emission from the controllable energy source passes through the objective lens, the objective lens may focus the emission from the controllable energy source at a different point than the focal point of a signal detected from the analyte and, in particular, at a point closer to the objective lens. | 2009-12-31 |
20090325218 | Determination of lipid, hydrocarbon or biopolymer content in microorganisms - A method for determining the content of a bioproduct in a cell culture comprises
| 2009-12-31 |
20090325219 | DEVICE, SYSTEM AND ASSAY FOR MEASURING CELL MOTILITY - The present application relates to a device and system for measuring cell motility comprising a supporting substrate which includes at least one cell receiving zone and an agent receiving matrix provided on a matrix receiving zone, wherein interposed between the cell receiving zone and the agent receiving matrix is a bridging portion. In addition, methods of using said device and system and a kit relating to the device is provided. | 2009-12-31 |
20090325220 | METHODS AND APPARATUS FOR STERILITY TESTING - Methods and apparatus for testing the sterility of a product to be administered to a patient. The sterility of the product is tested by monitoring the pH change of a culture medium in contact with any culturable organisms (e.g., bacteria) that are collected by filtration of the product to be tested. | 2009-12-31 |
20090325221 | Temporary Tattoo Decals for Detecting the Presence of an Analyte - Temporary tattoos to be applied to the skin of a wearer for the detection of an analyte are generally disclosed. The temporary tattoo can be applied to the skin via a temporary tattoo decal. The temporary tattoo can indicate the presence of an analyte by displaying a certain spectral response (e.g., color change) in the presence of a targeted analyte. This change in color can indicate to the wearer and/or caregiver the presence of the targeted analyte in real-time. | 2009-12-31 |
20090325222 | Tissue Sample Preparation and MALDI MS Imaging Thereof - Aspects of the present invention relate to a method for the preparation of samples for MALDI MS imaging. Certain embodiments relate to a method of matrix deposition for samples, wherein tissue sections are prepared via a synergistic combination of fixation with matrix. In certain embodiments, tissue is fixed with cold solvent, according to well-established histology protocols, and in the presence of matrix, allowing for high resolution spatial mapping of protein, lipid, sugar, and/or nucleic acid distribution. In certain embodiments, the present invention relates to fixation with matrix of whole organisms. In certain embodiments, animals are perfused with fixation and matrix mixtures, which allows for direct mass spectrometry analysis. | 2009-12-31 |
20090325223 | MAGNETIC IMMUNOHISTOCHEMICAL STAINING DEVICE AND METHODS OF USE - The subject invention concerns a device for use in staining samples or tissues of interests on slides. The subject invention also concerns methods of using the device to stain a specimen on a slide or to detect antibody and proteins of interest of a tissue section. | 2009-12-31 |
20090325224 | PROCESS FOR PREPARATION OF OPTICALLY ACTIVE N-PROTECTED 3-AMINOPYRROLIDINE OR OPTICALLY ACTIVE N-PROTECTED 3-AMINOPIPERIDINE AND THE CORRESPONDING KETONES BY OPTICAL RESOLUTION OF THE RACEMIC AMINE MIXTURES EMPLOYING A BACTERIAL OMEGA-TRANSAMINASE - The present invention relates to the production of optically active amines, which can be used as intermediate products in a synthesis of for instance pharmaceutical products. | 2009-12-31 |
20090325225 | METHOD FOR THE PRODUCTION OF (4S)-3,4-DIHYDROXY-2,6,6-TRIMETHYL-CYCLOHEX-2-ENONE AND DERIVATIVES THEREOF - The present invention relates to a method for preparing optically active (4S)-4-hydroxy-2,6,6-trimethylcyclohex-2-en-1-one derivatives of the formula (I) and a method for preparing (3S,3′S)-astaxanthin of the formula (III) comprising the first-mentioned method. | 2009-12-31 |
20090325226 | Methods and Compositions for Producing Active Vitamin K-Dependent Proteins - The present invention provides a method of identifying a human subject having increased or decreased sensitivity to warfarin, comprising detecting in the subject the presence of a single nucleotide polymorphism in the VKOR gene, wherein the single nucleotide polymorphism is correlated with increased or decreased sensitivity to warfarin, thereby identifying the subject having increased or decreased sensitivity to warfarin. | 2009-12-31 |
20090325227 | C-TERMINUS MODIFICATION METHOD, C-TERMINUS IMMOBILIZATION METHOD AND ANALYSIS METHOD FOR PROTEIN OR PEPTIDE - The present invention provides a method for inexpensively, easily and efficiently modifying the C-terminus of a protein or peptide; a method for easily and reliably isolating a C-terminal peptide fragment of a protein or peptide; and a method for rapidly, accurately and reliably determining an amino acid sequence of a protein or peptide by using a mass spectroscope. A method comprising the step of adding a formylation reagent and a catalyst to a protein or peptide to convert a carboxyl group into an aldehyde group. A method comprising the step of reacting a nucleophilic reagent with the aldehyde group to modify the C-terminus of the protein or peptide. A method comprising the step of reacting a support having a nucleophilic group to immobilize the protein or peptide. A method comprising the step of fragmenting the immobilized protein or peptide, washing the support, and isolating the C-terminal peptide fragment from the support. A method comprising the step of subjecting the isolated C-terminal peptide fragment to mass spectrometry and determining an amino acid sequence. | 2009-12-31 |
20090325228 | FUNGAL CELL WALL SYNTHESIS GENE - A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed. Therefore, through the novel compounds, the present invention showed that antifungal agents having a novel mechanism, i.e. inhibiting the process that transports GPI-anchored proteins to the cell wall, could be achieved. | 2009-12-31 |
20090325229 | Process for Globular Adiponectin Production - This invention relates to methods for use in industrial production of recombinant globular Adiponectin (gAdiponectin). Specifically, the present invention provides a purification process suitable for production of high amounts of pure gAdiponectin. gAdiponectin is expressed in | 2009-12-31 |
20090325230 | PROTEIN EXPRESSION SYSTEMS - The present invention provides an improved expression system for the production of recombinant polypeptides utilizing auxotrophic selectable markers. In addition, the present invention provides improved recombinant protein production in host cells through the improved regulation of expression. | 2009-12-31 |
20090325231 | THERMOSTABLE L-RIBOSE ISOMERASE AND METHOD FOR PRODUCING SAME AND USE OF SAME - Object: To provide a thermostable L-ribose isomerase. | 2009-12-31 |
20090325232 | Methods for prenatal diagnosis of chromosomal abnormalities - Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome. | 2009-12-31 |
20090325233 | METHOD OF PRODUCING A NUCLEIC ACID - The present invention provides a method of producing a nucleic acid at high reaction efficiency and high reproducibility with a decreased variation in yield and purity among different reaction lots. A nucleic acid synthesis reaction is carried out on a first solid phase carrier capable of supporting nucleic acid synthesis contained in a solid phase carrier mixture comprising the first solid phase carrier and a second solid phase carrier that does not support nucleic acid synthesis. | 2009-12-31 |
20090325234 | Apparatus and method for a continuous rapid thermal cycle system - A thermal cycle system and method suitable for mass production of DNA comprising a temperature control body having at least two sectors. Each sector has at least one heater, cooler, or other means for changing temperature. A path traverses the sectors in a cyclical fashion. In use, a piece of tubing or other means for conveying is placed along the path and a reaction mixture is pumped or otherwise moved along the path such that the reaction mixture is repetitively heated or cooled to varying temperatures as the reaction mixture cyclically traverses the sectors. The reaction mixture thereby reacts to form a product. In particular, polymerase chain reaction reactants may continuously be pumped through the tubing to amplify DNA. The temperature control body is preferably a single aluminum cylinder with a grooved channel circling around its exterior surface, and preferably has wedge-shaped or pie-shaped sectors separated by a thermal barrier. | 2009-12-31 |
20090325235 | THERMOACTIVE SIVagm SAB REVERSE TRANSCRIPTASE - Methods and kits performing reverse transcription and RT-PCR reactions having high fidelity, processivity and DNA polymerase activity are described. The methods involve performance of reverse transcription at an increased temperature with a reverse transcriptase from Simian Immunodeficiency Virus-agm.sab or a variation thereof. The kits of the present invention include a reverse transcriptase from Simian Immunodeficiency Virus-agm.sab or a variation thereof, a DNA polymerase capable of amplifying cDNA under conditions suitable for polymerase chain reaction, and the reagents necessary to carry out both processes. | 2009-12-31 |
20090325236 | Optical sorting method - The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention. | 2009-12-31 |
20090325237 | Method of Analyzing a BRCA2 Gene in a Human Subject - Five novel DNA and protein sequences have been determined for the BRCA2 gene, as have been ten polymorphic sites and their rates of occurrence in the normal alleles of BRCA2. The sequences BRCA2 | 2009-12-31 |
20090325238 | Method of Analyzing a BRCA2 Gene in a Human Subject - Five novel DNA and protein sequences have been determined for the BRCA2 gene, as have been ten polymorphic sites and their rates of occurrence in the normal alleles of BRCA2. The sequences BRCA2 | 2009-12-31 |
20090325239 | METHODS FOR NUCLEIC ACID MAPPING AND IDENTIFICATION OF FINE-STRUCTURAL-VARIATIONS IN NUCLEIC ACIDS - A method of juxtaposing sequence tags (GVTs) that are unique positional markers along the length of a population of target nucleic acid molecules is provided, the method comprising: fragmenting the target nucleic acid molecule to form target DNA insert; ligating the target DNA insert to a DNA vector or backbone to create a circular molecule; digesting the target DNA insert endonuclease to cleave the target DNA insert at a distance from each end of the target DNA insert yielding two GVTs comprising terminal sequences of the target DNA insert attached to an undigested linear backbone; recircularizing the linear backbone with the attached GVTs to obtain a circular DNA containing a GVT-pair having two juxtaposed GVTs; and recovering the GVT-pair DNA by nucleic acid amplification or digestion with endonuclease having sites flanking the GVT-pair. Cosmid vectors are provided for creating GVT-pairs of ˜45- to 50-kb separation sequencable by next-generation DNA sequencers. | 2009-12-31 |
20090325240 | PRODUCTION AND USE OF PLANT DEGRADING MATERIALS - Disclosed herein are materials useful for degrading plant biomass material. In exemplary embodiments, the plant material comprises one or more enzymes that are expressed in plants and/or bacteria. Specifically exemplified herein are plant degrading enzymes expressed in chloroplasts. The chloroplast expressed enzymes may be provided as cocktails for use in conjunction with conventional methods of converting biomass into biofuels, such as cellulosic ethanol. | 2009-12-31 |
20090325241 | SUGAR TRANSPORT SEQUENCES, YEAST STRAINS HAVING IMPROVED SUGAR UPTAKE, AND METHODS OF USE - Disclosed are nucleic acid constructs comprising coding sequences operably linked to a promoter not natively associated with the coding sequence. The coding sequences encode | 2009-12-31 |
20090325242 | Alleles of the zwf gene from coryneform bacteria - The invention relates to mutants and alleles of the zwf gene of coryneform bacteria, which encode variants of the Zwf subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles. | 2009-12-31 |
20090325243 | Method for producing amino acids using glycerol - The present invention relates to an amino acid-producing microorganism capable of simultaneously utilizing glycerol as a carbon source, a method for preparing the microorganism, and a method for producing amino acids using the microorganism. According to the present invention, amino acids can be efficiently produced using a byproduct of biodiesel production, glycerol, thereby substituting a cheaper material for the conventional fermentation materials such as glucose. | 2009-12-31 |
20090325244 | METHOD OF INCREASING GENE EXPRESSION USING MODIFIED CODON USAGE - The present invention relates to a method of increasing the amount of at least one polypeptide in the host cell by expressing a modified nucleotide sequence encoding for a polypeptide in a host cell with said modified nucleotide sequence being derived from a different non-modified nucleotide sequence encoding for a polypeptide of identical amino acid sequence such that the codon usage of the modified nucleotide sequence is adjusted to the codon usage of abundant proteins in the host cell. | 2009-12-31 |
20090325245 | Ethanolamine Production by Fermentation - The present invention provides a bacterium and a method for the biological production of ethanolamine from a fermentable carbon source. In one aspect of the present invention, a process for the conversion of glucose to ethanolamine is achieved by the use of a recombinant bacterium transformed i) to express a serine decarboxylase enzyme to convert serine to ethanolamine ii) to inactivate the ethanolamine consuming pathways and iii) to increase 3-phosphoglycerate availability. In another aspect of the present invention, the process for the production of ethanolamine from glucose using a recombinant | 2009-12-31 |
20090325246 | PROCESS FOR PRODUCTION OF BETAINE - According to the present invention, by using 4-halogeno-3-hydroxybutanamide as a substrate in quaternary amination reaction with trialkylamine which is an important step in betaine (such as carnitine) preparation processes, it becomes possible to reduce the production of crotonic acid derivatives (the major by-product) greatly compared to conventional processes. Consequently, it becomes possible to prepare a betaine, such as carnitine, at a high yield. | 2009-12-31 |
20090325247 | METHODS FOR PRODUCING CERAMIDE USING TRANSFORMED YEAST - The present invention provides methods for producing human ceramide in a yeast cell. | 2009-12-31 |
20090325248 | Microbiological Production of 3-Hydroxypropionic Acid - The present invention relates to a cell which is genetically modified in relation to its wild type and which exhibits at least one of the properties a) or b):
| 2009-12-31 |
20090325249 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 2009-12-31 |
20090325250 | SEQUESTRATION OF FORMALDEHYDE TO STABILIZE NITRILASE SPECIFIC ACTIVITY WHEN CONVERTING GLYCOLONITRILE TO GLYCOLIC ACID - A process is provided to improve the specific activity of an enzyme catalyst having nitrilase activity when converting glycolonitrile to glycolic acid under aqueous reaction conditions. Inclusion of an effective amount of at least one amine protectant improves the specific activity and catalytic productivity of the enzyme catalyst. | 2009-12-31 |
20090325251 | Methods and Systems for Biomass Conversion to Carboxylic Acids and Alcohols - The disclosure includes a method, process and apparatus for the conversion of biomass to carboxylic acids and/or primary alcohols. The system may include a pretreatment/fermentation subsystem operable to produce a fermentation broth containing carboxylic acid salts from biomass, such as lignocellulosic biomass. The system may also include a dewatering subsystem operable to remove excess water from the fermentation broth to produce a concentrated product. The system may also includes an acid springing subsystem operable to produce a mixed carboxylic acid product. The system may also include a hydrogenation subsystem operable to produce an alcohol mixture, such as a mixture containing primary alcohols. Methods of operating this system or other systems to obtain a carboxylic acid or alcohol mixture are also provided. | 2009-12-31 |
20090325252 | Process of treating cellulosic biomass material to produce ethanol - This invention relates to a process of treating a cellulosic biomass material to produce ethanol, at atmospheric temperatures and pressures. This process does not utilize high pressures or temperatures. The process includes the steps of: (a) exposing the cellulosic biomass material to acetic acid [C | 2009-12-31 |
20090325253 | METHODS AND SYSTEMS FOR PRODUCTION OF BIOFUELS AND BIOENERGY PRODUCTS FROM SEWAGE SLUDGE, INCLUDING RECALCITRANT SLUDGE - The present invention provides methods and systems (SLUDFUEL system) for producing biofuel and bioenergy products using, as starting raw material, municipal, industrial, and/or farm sewage sludge, including recalcitrant sludge containing high concentrations of heavy metals, and produced after waste treatment. In accordance with the invention, municipal, industrial, and farm sewage sludge, including recalcitrant sludge, can serve as a carbon source to support the metabolism of synthetic microorganisms to produce biofuels and bioenergy products. | 2009-12-31 |
20090325254 | HIGHLY ACTIVE XYLOSE REDUCTASE FROM NEUROSPORA CRASSA - A new xylose reductase encoding gene from | 2009-12-31 |
20090325255 | PROCESS FOR OVER-PRODUCTION OF HYDROGEN - The present invention provides a process of increasing production of hydrogen during fermentation process and also an electro-biochemical is designed to achieve higher hydrogen production. | 2009-12-31 |
20090325256 | METHOD FOR CELL PATTERNING - Provided is a method for cell patterning, using an electrode substrate including a plurality of electrodes and a cell culture substrate disposed so as to face the electrode substrate, the method comprising the steps of:
| 2009-12-31 |
20090325257 | Method for Influencing Living Cells Through Cell-Surface Interaction - The invention relates to a method for influencing living cells through cell-surface interaction where, for the cell-surface interaction, bioactive material is applied to magnetic carrier material, then this magnetic carrier material is applied to a magnetic carrier substrate, and this carrier substrate is combined with the living cells. The magnetic domain arrangement in the substrate can be altered by means of an external magnetic field in such a way that the structure formed by the magnetic carrier material is likewise changed. An in vitro alteration of the structure of the substrate is thus possible. | 2009-12-31 |
20090325258 | Magnetic particle holding carrier and method for preparing the same - Provided is a magnetic particle holding carrier enabling automatization of treatment of a biological substance such as a protein by improving dispersibility of nano-size magnetic particles and suppressing nonspecific adsorption onto the wall of a container such as a pipette tip without damaging the properties of the nano-size magnetic particles such as a large solid-phase area and ability to arbitrarily design a functional protein, and provide a method for preparing the same. The magnetic particle holding carrier is formed of a micro-size nonmagnetic carrier and a plurality of nano-size magnetic particles bound to the carrier. | 2009-12-31 |
20090325259 | USE OF ADHESION MOLECULES AS BOND STRESS-ENHANCED NANOSCALE BINDING SWITCHES - Methods, compositions and devices are provided based on changing the binding strength of an adhesion molecule to a ligand by changing the force exerted on the bound complex between adhesion molecule and ligand, for example by changing the shear stress acting on the complex. The adhesion molecules and their ligands of this invention bind more tightly when a force-activated bond stress, such as shear force, applied to the adhesion molecules is increased, and bond less tightly when the stress is decreased. The adhesion molecules can be isolated from their sources in nature or can remain attached to their natural sources. They can be engineered, e.g., by altering their amino acid sequences or by binding to antibodies or other particles, to alter their binding properties. They can be attached to a wide range of substrates including particles and device surfaces to form adhesive systems which are capable of sticking to other particles and/or device surfaces to which ligands for the adhesion molecules have been attached. The adhesion molecules and ligands described herein can be used to control binding and release of components of an adhesive system by increasing or decreasing the force-activated bond stresses applied to the adhesion molecules. | 2009-12-31 |
20090325260 | CIS REACTIVE OXYGEN QUENCHERS INTEGRATED INTO LINKERS - The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates. | 2009-12-31 |
20090325261 | Passivation of nerve agents by surface modified enzymes stabilized by non-covalent immobilization on robust, stable particles - Enzymes are modified by incorporating anchor sites for linking the enzymes to a target surface without destroying the catalytic activity of the enzymes. A stable carrier to accommodate and bind the selected enzyme is constructed, and the enzyme is non-covalently liked to the carrier, generally through metal salts of iminodiacetate | 2009-12-31 |
20090325262 | Immobilization of biological molecules onto surfaces coated with monolayers - The present invention provides an article for immobilizing functional organic biomolecules through a covalent bond to a thiolate monolayer on a coinage metal surface. Also provided are methods for making the article and method for the immobilization of functional organic biomolecules on the article. The thiolate monolayer contains two moieties, one having an inert group that is resistant to reacting with biomolecules and one having a covalent bond forming group that reacts with the functional organic biomolecule to covalently immobilize it on the monolayer. | 2009-12-31 |
20090325263 | PREPARATION OF GLASSIFIED BIOLOGICAL REAGENTS - The invention related to a method of making a dried reagent preparation, comprising the steps of: providing an aqueous solution of at least one buffered biological reagent; mixing a glass forming filler material with the buffered reagent solution to form a mixture wherein the concentration of the filler material is sufficient to facilitate formation of a glassy, porous composition; dispensing the mixture in the form of substantially uniform droplets into wells of a multi-well container, wherein a single droplet is dispensed into each well; drying the droplets in the container to form the reagent preparation. The reagent preparation is water soluble and has a Tg sufficient for room temperature stability. | 2009-12-31 |
20090325264 | Engineered Delta-15-Fatty Acid Desaturases - The present invention provides engineered fatty acid desaturase molecules preferring Gamma Linolenic Acid (GLA) over Linoleic Acid (LA) as a substrate. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds comprising the desaturase molecule, and methods for preparing and using the same. In particular, the disclosed engineered desaturase molecules are capable of altering the omega-3 fatty acid profiles in plants and plant parts. | 2009-12-31 |
20090325265 | High eicosapentaenoic acid producing strains of yarrowia lipolytica - Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from | 2009-12-31 |
20090325266 | Production Of Peracids Using An Enzyme Having Perhydrolysis Activity - A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided. | 2009-12-31 |
20090325267 | Modified xylanases exhibiting increased thermophilicity and alkalophilicity - The present invention pertains to modified xylanase enzymes that exhibit increased thermostability and alkalophilicity, when compared with their native counterparts. Several modified xylanases exhibiting these properties are disclosed including xylanases with at least one modification at amino acid position 10, 27, 29, 75, 104, 105, 125, 129, 132, 135, 144, 157, 161, 162 or 165, or a combination thereof. Also included within the present invention is a modified xylanase that comprises at least one substituted amino acid residue and that may be characterized as having a maximum effective temperature (MET) between about 69° C. and about 78° C., wherein the modified xylanase is a Family 11 xylanase obtained from a | 2009-12-31 |
20090325268 | PROLINE-SPECIFIC PROTEASES FREE FROM AMYLOLYTIC ACTIVITIES - The invention relates to a proline-specific protease preparation free from amylolytic activity and a purification method for obtaining the enzyme preparation according to the invention. | 2009-12-31 |
20090325269 | Bacteria/RNA extraction device - Apparatus and method for collection of a target material from a liquid sample comprising target species that contain the target material. The apparatus comprises a fluid flow conduit in communication with a filter medium having a pore size adapted to retain target species thereon and pass, as filtrate, lysate containing the desired target material. A lysing agent conduit communicates with the fluid flow conduit and delivers lysing agent to the filter medium to lyse the target cells thereby releasing the desired intracellular target material. The lysing agent may be recirculated through the filter medium for a sufficient time to permit sufficient quantity of lysate to circulate through the system for lysate collection and subsequent assay. | 2009-12-31 |
20090325270 | USE OF 2-HYDROXY-5-OXOPROLINE IN CONJUNCTION WITH ALGAE - Provided herein are exemplary methods for the use of 2-hydroxy-5-oxoproline in conjunction with algae. One exemplary method includes applying an effective amount of 2-hydroxy-5-oxoproline to algae in an aqueous environment to accelerate creation of a high-cell density of the algae. The effective amount of the 2-hydroxy-5-oxoproline may be approximately 0.1 grams per liter of the aqueous environment, or up to approximately 0.1 grams per liter of the aqueous environment. The effective amount of the 2-hydroxy-5-oxoproline may be applied to the aqueous environment at or near a same time, or applied to the aqueous environment over a period of time. Exemplary algae cultivation systems are also provided herein. One exemplary system includes an aqueous environment having a pale-green mutant | 2009-12-31 |
20090325271 | METHOD FOR BIO-ASSISTED TREATMENT OF HYDROCARBON CONTAMINATED SOIL - Disclosed herein is a bio-assisted method for treatment of hydrocarbon contaminated soil employing novel microbes which are capable of decontaminating hydrocarbon contaminated soil having free flowing water or in slurry form or having large amount of gravels. The method comprises adding hydrocarbon releasing microbes followed by adding the microbes capable of degrading the released hydrocarbon to decontaminate the soil, wherein said microbes are grown separately in suitable vessels containing a suitable nutrient medium. | 2009-12-31 |
20090325272 | Nucleic Acid Sample Preparation by Exclusion of DNA - Devices and methods are provided for separation of nucleic acids from waste materials in a biological sample, and then reacting the separated nucleic acids. In some embodiments, the methods comprise mixing a cell lysate having nucleic acids and waste materials with a waste-binding matrix to capture the cellular waste materials from the lysate. The waste-binding matrix can comprise hydrophilic and/or hydrophobic size-exclusion, ion-exchange particles. In some embodiments, the device comprises a substrate comprising a fluid processing pathway in which nucleic acid sample preparation occurs prior to a downstream genotyping reaction. | 2009-12-31 |
20090325273 | MULTI-FUNCTIONAL TREATING DEVICE FOR TREATING PATHOLOGICAL TISSUE SECTIONS - A multi-functional treating device for pathological tissue sections includes a main body provided with at least one accommodating chamber formed in its top portion and a central controller. A water bath, an oven, a paraffin scrubber and a section baking rack are respectively installed in each of the accommodating chambers. The central controller is provided with plural control buttons, which are respectively connected with an electrothermal device installed under the paraffin scrubber, the water bath, the oven and the section baking rack respectively. Thus, the multi-functional treating device can simultaneously do jobs of scrubbing off extra paraffin, wetting tissue sections, primarily drying up tissue sections, and drying up slides carrying the sections, saving a lot of space. | 2009-12-31 |
20090325274 | ANALYZER - An analyzer comprising: a first specimen holder configured to hold a plurality of first specimen containers; a conveying assembly for conveying the first specimen containers held in the first specimen holder; a second specimen holder arranged at a position higher than an upper end of the first specimen containers held in the first specimen holder; a holder moving assembly for moving the second specimen holder so as to pass the upper side of at least one of the first specimen containers held in the first specimen holder; a container transferring assembly for transferring at least one of the first specimen containers from the first specimen holder to the second specimen holder; and a controller for controlling the holder moving assembly and the container transferring assembly, is disclosed. | 2009-12-31 |
20090325275 | CELL MANIPULATION AND CULTIVATION EQUIPMENTS FOR THE PRODUCTION OF CELL THERAPY PRODUCTS - Cell manipulation and cultivation equipment for the production of cell therapy products has developed for preventing contamination of cells and improving the utility of a space. The equipment includes a room for the production and testing of cell therapy products, the room having an inner space enclosed in all sides and an outer entrance/exit door provided at any one side thereof, an L-shaped partition installed in a position of the room for the production and testing of cell therapy products, the partition having an inner entrance/exit door provided at any one side thereof, and a clean bench device placed inside the partition, the clean bench device including first, second, and third clean benches for preventing contamination of cells and increasing the utility of a space to the maximum extent. The operating of the clean room and clean bench has advantages to prevent contamination and improving the quality and reliability of cell therapy products. | 2009-12-31 |
20090325276 | INTEGRATED MICROFLUIDIC ASSAY DEVICES AND METHODS - Combinations of microfluidic diagnostic testing modules for simultaneous evaluations of serological and molecular biological targets are provided, and include panel testing for both antibodies (or antigens) and nucleic acid targets in one single-use device. These improvements are directed to evaluating the overall progress and activity of a pathogenic process in real time, at the point of care, not merely the presence or absence of a particular diagnostic marker, which can often be incomplete or misleading. | 2009-12-31 |
20090325277 | Thermal Device, System, and Method, for Fluid Processing Device - A device, system, and method are provided for thermally treating a fluid processing device. According to various embodiments, a system is provided that can include a thermal device and a fluid processing device holder. The thermal device can include a first block having a thermal conductivity greater than 0.5 Watt per centimeter Kelvin (W/cm·K), a second block having a thermal conductivity greater than 0.5 W/cm·K, and a heat-pump device disposed between the first block and the second block. The heat-pump device can transfer thermal energy from at least one of the first block and the second block to the other of the first block and the second block. The fluid processing device holder can hold a fluid processing device in a heat-transfer position with respect to the first block and the second block. The fluid processing device can be a microfluidic device. | 2009-12-31 |
20090325278 | Reaction System for Performing in the Amplification of Nucleic Acids - A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions. | 2009-12-31 |
20090325279 | PROCESS AND DEVICE FOR PRODUCING REAGENT CARRIERS - The invention concerns a process for producing reagent carriers having a binding capability that are suitable for determining analytes in liquids, wherein the process comprises at least one step of treating a respective reagent carrier body in particular by transferring material between a treatment device and the reagent carrier body in a preparation device and wherein this treatment step takes place on the moving reagent carrier body during its transport in the preparation device or during a movement of the treatment device relative to the reagent carrier body in the preparation device. | 2009-12-31 |