52nd week of 2017 patent applcation highlights part 27 |
Patent application number | Title | Published |
20170369859 | METHODS OF COMBINED BIOPROCESSING AND RELATED MICROORGANISMS, THERMOPHILIC AND/OR ACIDOPHILIC ENZYMES, AND NUCLEIC ACIDS ENCODING SAID ENZYMES - A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from | 2017-12-28 |
20170369860 | Endoglucanase Variants and Polynucleotides Encoding Same - The present invention relates to endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, expression vectors, and recombinant host cells comprising the polynucleotides; and methods of using the variants. | 2017-12-28 |
20170369861 | MUTATED FACTOR X POLYPEPTIDES AND USES THEREOF FOR THE TREATMENT OF HAEMOPHILIA - The present invention relates to mutated factor (FX) polypeptides and uses thereof for the treatment of haemophilia. In particular, the present invention relates to a mutated factor X (FX) polypeptide wherein the heavy chain comprises at least one mutation selected from the group consisting of: —the mutation which consists of the substitution of the glutamic acid residue (E) at position 255 of Seq. ID No. 1 by a glutamine residue (Q), an asparagine residue (N), a serine residue (S), an alanine residue (A), or a tyrosine residue (Y); —the mutation which consists of the substitution of the glutamic acid residue (E) at position 256 of Seq. ID No. 1 by a glutamine residue (Q); and —the mutation which consists of the substitution of the glutamic acid residue (E) at position 258 of Seq. ID No. 1 by a glutamine residue (Q); | 2017-12-28 |
20170369862 | PREPARATION OF FACTOR XA DERIVATIVES - The present disclosure provides methods for manufacturing a fXa derivative protein at large scale leading to high yield of highly pure protein product. The method may include adding a detergent to a sample that contains a polynucleotide construct encoding the protein and purifying the protein through a soybean trypsin inhibitor (STI)-based affinity chromatograph, an ion exchange and mixed mode chromatograph and a hydrophobic interaction. | 2017-12-28 |
20170369863 | GENETICALLY MODIFIED PHENYLPYRUVATE DECARBOXYLASE, PROCESSES TO PREPARE, AND USES THEREOF - Modification of the amino acid sequence of a phenylpyruvate decarboxylase from | 2017-12-28 |
20170369864 | INHIBITION AND DIAGNOSTICS OF EMERGING TETRACYCLINE RESISTANCE ENZYMES - The present disclosure provides compositions and methods for treating bacterial infections in a subject. The methods comprise administering a compound that binds a FAD-dependent flavoenzyme and a tetracycline, analog, derivative, or pharmaceutically acceptable salt thereof. | 2017-12-28 |
20170369865 | ACOUSTOPHORESIS DEVICE WITH DUAL ACOUSTOPHORETIC CHAMBER - An acoustophoresis device includes an acoustic chamber with a piezoelectric element located within its volume. The piezoelectric element vibrates and generates acoustic standing waves from both sides, so that particles can be separated from fluid passing through the acoustic chamber. This permits the element to be cooled more efficiently, reducing transient heat loads in the fluid traveling through the device. | 2017-12-28 |
20170369866 | COMPOSITIONS AND METHODS FOR EFFICIENT GENE EDITING IN E. COLI USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS IN COMBINATION WITH CIRCULAR POLYNUCLEOTIDE MODIFICATION TEMPLATES - Compositions and methods are provided for genome modification of a target sequence in the genome of an | 2017-12-28 |
20170369867 | MIRAC PROTEINS - This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at the wild type normal physiological conditions. For example, evolved proteins are virtually inactive at body temperature, but are active at lower temperatures. | 2017-12-28 |
20170369868 | METHOD FOR ENGINEERING IMMUNOGLOBULINS - The present invention relates to a method for engineering an immunoglobulin comprising a variable domain and at least one modification in at least two structural loops of said immunoglobulin and determining the binding of said immunoglobulin to an epitope of an antigen, wherein the unmodified immunoglobulin does not significantly bind to said epitope, comprising the steps of: providing a nucleic acid encoding an immunoglobulin comprising at least two structural loops, modifying at least one nucleotide residue of each of said structural loops, transferring said modified nucleic acid in an expression System, expressing said modified immunoglobulin, contacting the expressed modified immunoglobulin with an epitope, and determining whether said modified immunoglobulin binds to said epitope, immunoglobulins produced by such a method and libraries of immunoglobulins. | 2017-12-28 |
20170369869 | EXPRESSION AND SECRETION SYSTEM - The invention provides an expression and secretion system, and methods of using the same, for the expression and secretion of one fusion protein in prokaryotic cells and a second fusion protein in eukaryotic cells. Also provided herein are nucleic acid molecules, vectors and host cells comprising such vectors and nucleic acid molecules. | 2017-12-28 |
20170369870 | METHODS FOR GENERATING BARCODED COMBINATORIAL LIBRARIES - Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases. | 2017-12-28 |
20170369871 | INCORPORATION OF UNNATURAL NUCLEOTIDES AND METHODS THEREOF - Disclosed herein are methods, composition and kits for the synthesis of proteins which comprises unnatural amino acids that utilize a mutant tRNA, wherein the mutant tRNA comprises a mutant anticodon sequence. And an additional method comprises generating nucleic acids that contain an expanded genetic alphabet. | 2017-12-28 |
20170369872 | REVERSIR TM COMPOUNDS - The present invention relates, in general to agents that modulate the pharmacological activity of conjugated siRNAs. | 2017-12-28 |
20170369873 | PARENTAL RNAI SUPPRESSION OF CHROMATIN REMODELING GENES TO CONTROL COLEOPTERAN PESTS - This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of coleopteran pests, and the plant cells and plants obtained thereby. | 2017-12-28 |
20170369874 | RAAV-BASED COMPOSITIONS AND METHODS FOR TREATING ALPHA-1 ANTI-TRYPSIN DEFICIENCIES - The invention relates to isolated nucleic acids and rAAV-based compositions, methods and kits useful for treating genetic diseases (e.g., alpha-1 antitrypsin deficiency). | 2017-12-28 |
20170369875 | EXON SKIPPING COMPOSITIONS FOR TREATING MUSCULAR DYSTROPHY - Antisense molecules capable of binding to a selected target site in the human dystrophin gene to induce exon 53 skipping are described. | 2017-12-28 |
20170369876 | EXON SKIPPING COMPOSITIONS FOR TREATING MUSCULAR DYSTROPHY - Antisense molecules capable of binding to a selected target site in the human dystrophin gene to induce exon 44 skipping are described. | 2017-12-28 |
20170369877 | REDUCTION OF OFF-TARGET RNA INTERFERENCE TOXICITY - The present invention is directed to RNA interference (RNAi) molecules targeted against a nucleic acid sequence, and methods of using these RNAi molecules to reduce off-target toxicity. | 2017-12-28 |
20170369878 | MICRORNA COMBINATIONS FOR ANTI-CANCER THERAPEUTICS - Described herein are methods and compositions of combinations of microRNAs that enhance the sensitivity of cancer cells to chemotherapeutic agents or reduce proliferation of cancer cells. Also described herein are methods for the identification of combinations of microRNAs that result in desired effects. | 2017-12-28 |
20170369879 | METHODS FOR TREATMENT OF ALPORT SYNDROME - Provided herein are methods for the treatment of Alport Syndrome, using modified oligonucleotides targeted to miR-21. In certain embodiments, a modified oligonucleotide targeted to miR-21 improves kidney function and/or reduces fibrosis in subjects having Alport Syndrome. In certain embodiments, administration of a modified oligonucleotide targeted to miR-21 delays the onset of end-stage renal disease in a subject having Alport Syndrome. In certain embodiments, a modified oligonucleotide targeted to miR-21 delays the need for dialysis or kidney transplant in a subject having Alport Syndrome. | 2017-12-28 |
20170369880 | COMPOSITIONS COMPRISING ALTERNATING 2'-MODIFIED NUCLEOSIDES FOR USE IN GENE MODULATION - The present invention provides compositions comprising at least one oligomeric compound comprising an alternating motif and further include a region that is complementary to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression. | 2017-12-28 |
20170369881 | RNA Targeting in Alpha-Synucleinopathies - Therapies and assays to screen for small molecules that can have therapeutic use in the control of neurodegenerative diseases such as Parkinson's and other alphα-synucleinopathies. | 2017-12-28 |
20170369882 | FULLY STABILIZED ASYMMETRIC SIRNA - Provided herein are self-delivering oligonucleotides that are characterized by efficient RISC entry, minimum immune response and off-target effects, efficient cellular uptake without formulation, and efficient and specific tissue distribution. | 2017-12-28 |
20170369883 | MODULATION OF HEPATITIS B VIRUS (HBV) EXPRESSION - Disclosed herein are antisense compounds and methods for decreasing HBV mRNA, DNA and protein expression. Such methods, compounds, and compositions are useful to treat, prevent, or ameliorate HBV-related diseases, disorders or conditions. | 2017-12-28 |
20170369884 | MicroRNAs Sensitize Cancers to Therapy - The present invention concerns methods and compositions regarding one or more microRNAs or variants thereof that are provided to an individual for a variety of medical treatments, including sensitization to cancer therapy or prevention of a cancer to become sensitized to a cancer therapy. In specific embodiments, the microRNAs include miR-520a (including at least miR-520a-3p and miR-520-5p), miR-520g, miR-520h, and functional variants thereof. In some embodiments, the cancer is ovarian cancer, and in particular embodiments, the cancer therapy is platinum-based chemotherapy. | 2017-12-28 |
20170369885 | TREATMENT OF TH17 MEDIATED INFLAMMATORY DISEASES - The present invention provides methods and means to reduce inflammation associated with IRF-4, AP-1 and TH17 mediated diseases. In particular, the invention provides methods and means to treat multiple sclerosis, rheumatoid arthritis, inflammatory bowel disease, and psoriasis and related conditions. | 2017-12-28 |
20170369886 | Inhibin As Targetable Regulators Of Angiogenesis - Contact of endothelial cells with inhibitors of inhibin and/or the alpha subunit of inhibin can be utilized to modify the activity of endothelial cell expression products including SMAD 1/5. Methods can also include inhibiting Alk1 and/or endoglin as components of inhibin-activated pathway of SMAD 1/5 signaling. Methods can be combined with other anti-angiogenesis therapies such as anti-VEGF therapies. Methods can be utilized in treatment of inhibin-expressing cancers (e.g., ovarian cancer, pancreatic cancer) as well as other pathologies such as preeclampsia and PCOS. | 2017-12-28 |
20170369887 | Therapeutic compositions for breast cancer containing protein kinase D1 inhibitor - A method of inhibiting the growth of cancer stem cells, including administering an effective amount of a protein kinase D1 expression or activity inhibitor as an active ingredient to a subject having cancer is provided. Further, a method of treating cancer, including administering an effective amount of a protein kinase D1 expression or activity inhibitor, and antitumor agent as active ingredients to a subject having cancer is provided. Further, a method for measuring expression or activity of protein kinase D1 for providing information of breast cancer prognosis, including a step of measuring expression or activity of protein kinase D1 in cells or tissues isolated from a subject is provided. | 2017-12-28 |
20170369888 | ADAM12 Inhibitors and Their Use Against Inflammation-Induced Fibrosis - The present invention relates to the field of fibrosis and inflammation and more particularly to the use of ADAM12 (A Disintegrin and Metalloproteinase 12) inhibitors to prevent or treat inflammation-induced fibrosis. The present invention also relates to the use of ADAM12 as a marker for inflammation-induced fibrosis and to the ablation of ADAM12 expressing cells as therapeutic approach to interfere with the development of pro-fibrotic cells. | 2017-12-28 |
20170369889 | SYSTEM AND METHOD OF MODULAR CLONING - System for producing a nucleic acid construct of interest, said system comprising:
| 2017-12-28 |
20170369890 | METHODS FOR MAKING A SYNTHETIC GENE - Methods for making a synthetic gene are provided. The methods find use in optimizing a candidate gene nucleic acid sequence for expression in a selected target expression system. The method identifies stable or retained sequences in the candidate gene nucleic acid sequence, identifies disallowed sequences, develops a statistical model based on a whole genome, a partial genome, or transcriptome sequences of the target expression system, generates an optimized candidate gene nucleic acid sequence for use in the target expression system, and makes a synthetic gene comprising the optimized candidate gene nucleic acid sequence. The method allows for optimization of the candidate gene nucleic acid sequence without removing certain stable or retained sequences. Rather, the activity of these sites is positionally modulated or inactivated through upstream and downstream modifications of codons and/or sequence patterns. | 2017-12-28 |
20170369891 | FUNGAL GENOME MODIFICATION SYSTEMS AND METHODS OF USE - Compositions and methods are provided for genome modification at a target site in the genome of a fungal cell. Aspects of methods and compositions are drawn to a guide polynucleotide/Cas endonuclease system for promoting insertion of a donor DNA at a desired target site in a fungal host cell genome. | 2017-12-28 |
20170369892 | HORMONE DEGRADABLE CRISPR-BASED TRANSCRIPTION FACTORS - Synthetic signal transduction systems are provided. The synthetic signal transduction system may be a hormone degradable CRISPR-based transcription factor including a nuclease null Cas9 protein, a nuclear localization signal, a phytohormone degron, and a transcriptional regulation domain. Methods of generating non-naturally occurring plants are also provided. The methods may include expressing a synthetic signal transduction system in a plant. Non-naturally occurring plants formed by the methods are also provided. | 2017-12-28 |
20170369893 | Modified FRT Recombination Site Libraries and Methods of Use - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. Kits comprising the library populations of FRT sites are also provided, as are methods to make a library of modified FRT recombination sites. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest. | 2017-12-28 |
20170369894 | Transgenic Plants With Enhanced Traits - This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait. | 2017-12-28 |
20170369895 | BRASSICA NAPUS SEED SPECIFIC PROMOTERS IDENTIFIED BY MICROARRAY ANALYSIS - Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from | 2017-12-28 |
20170369896 | PLANT CYTOCHROME P450 - This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a | 2017-12-28 |
20170369897 | TRANSCRIPTION FACTORS AND METHOD FOR INCREASED FIBER LENGTH OF COTTON - The present invention includes a seed, a plant, a protoplast, a hybrid and methods of making the same of a cotton cultivar recombinantly modified overexpresses at least one of AtRAV1, AtRAV2 to confer longer fibers to transgenic cotton plants under drought conditions without an effect on yield. | 2017-12-28 |
20170369898 | SEQUENCES TO FACILITATE INCORPORATION OF DNA INTO THE GENOME OF AN ORGANISM - Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct. | 2017-12-28 |
20170369899 | MUTATED ACETOHYDROXYACID SYNTHASE GENES IN EUPHORBIACEAE AND PLANT MATERIAL COMPRISING SUCH GENES - Provided are mutated acetohydroxyacid synthase (AHAS) nucleic acids and the proteins encoded by the mutated nucleic acids. Also provided are cassava plants, cells, and seeds comprising the mutated genes. | 2017-12-28 |
20170369900 | SCN PLANTS AND METHODS FOR MAKING THE SAME - The invention relates to genes which may be utilized for resistance to soybean cyst nematode. More specifically the present disclosure relates to identification of gene(s) that can confer upon a soybean plant resistance to soybean cyst nematode (SCN) and methods to use these loci and genes to obtain soybean strains that are resistant to SCN. | 2017-12-28 |
20170369901 | METHODS AND COMPOSITIONS TO ENHANCE ACTIVITY OF CRY ENDOTOXINS - Methods and compositions for enhancing the resistance of plants to plant pests are provided. Chimeric pesticidal polypeptides and nucleic acid molecules encoding the chimeric pesticidal polypeptides are provided. The chimeric pesticidal polypeptides comprising a solubility-enhancing polypeptide operably linked to a polypeptide comprising pesticidal activity. The nucleic acid molecules can be used in expression cassettes for making transformed plants with enhanced resistance to plant pests. Further provided are transformed plants, plant tissues, plant cells, other host cells, and seeds as well as pesticidal compositions. | 2017-12-28 |
20170369902 | RESTORATION OF MALE FERTILITY IN WHEAT - Manipulation of male fertility in a polyploid species requires attention to the interaction of male-fertility alleles of multiple genomes. In hexaploid wheat, single-genome heterozygotes for Ms26 provide differential levels of male fertility across genomes. Hexaploid wheat homozygous for mutations in the Ms26 gene on the A, B, and D genomes is male-sterile. Male fertility may be restored by sufficient levels of expression of Ms26 using native Ms26 or a transgene, which may be native to wheat or to another species, or a combination of native and transgenic alleles. CRISPR/Cas9 technology may be used to generate mutations in Ms26 in wheat or rice. | 2017-12-28 |
20170369903 | GENE EXPRESSION SYSTEM USING STEALTHY RNA, AND GENE INTRODUCTION/EXPRESSION VECTOR INCLUDING SAID RNA - Simultaneous expression of a plurality of foreign genes by using a stealthy RNA gene expression system that is a complex that does not activate the innate immune mechanism and is formed from an RNA-dependent RNA polymerase, a single-strand RNA binding protein, and negative-sense single-strand RNAs including the following (1) to (8): (1) a target RNA sequence that codes for any protein or functional RNA; (2) an RNA sequence forming a noncoding region and derived from mRNA; (3) a transcription initiation signal sequence recognized by the RNA-dependent RNA polymerase; (4) a transcription termination signal sequence recognized by the polymerase; (5) an RNA sequence containing a replication origin recognized by the polymerase; (6) an RNA sequence that codes for the polymerase; (7) an RNA sequence that codes for a protein for regulating the activity of the polymerase; and (8) an RNA sequence that codes for the single-strand RNA binding protein. | 2017-12-28 |
20170369904 | METHODS FOR INCREASING CELL CULTURE TRANSFECTION EFFICIENCY AND CELLULAR REPROGRAMMING - The present invention describes a method for increasing transfection efficiency of cells. The present invention further provides a method for increasing the efficiency of stem cell reprogramming. | 2017-12-28 |
20170369905 | N-GLYCOSYLATION - The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses. | 2017-12-28 |
20170369906 | HIGHLY EFFICIENT ETHANOL-FERMENTATIVE YEAST - A highly efficient ethanol-fermentative yeast having high efficiency in ethanol production is provided without introducing a foreign gene. The highly efficient ethanol-fermentative yeast features a fermentative yeast effectively producing ethanol from pentose and hexose and being deposited to NITE Patent Microorganisms Depositary under the accession number NITE BP-01963. | 2017-12-28 |
20170369907 | METHODS OF PRODUCING 7-CARBON CHEMICALS VIA PYRUVATE AND SUCCINATE SEMIALDEHYDE ALDOL CONDENSATION - This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate. | 2017-12-28 |
20170369908 | POLYHYDROXYALKANOATE PRODUCTION METHODS AND MATERIALS AND MICROORGANISMS USED IN SAME - Embodiments of the invention relate generally to methods to generate microorganisms and/or microorganism cultures that exhibit the ability to produce polyhydroxyalkanoates (PHA) from carbon sources at high efficiencies. In several embodiments, preferential expression of, or preferential growth of microorganisms utilizing certain metabolic pathways, enables the high efficiency PHA production from carbon-containing gases or materials. Several embodiments relate to the microorganism cultures, and/or microorganisms isolated therefrom. | 2017-12-28 |
20170369909 | OMEGA-3 FATTY-ACID DESATURASE AND METHOD FOR PRODUCING EICOSAPENTAENOIC ACID - Provided is | 2017-12-28 |
20170369910 | OLEIC ACID PRODUCTION IN YEAST - Disclosed are transformed cells comprising one or more genetic modifications that affect the lipid content of the cell, e.g., by increasing the concentration of oleic acid in the cell relative to an unmodified cell of the same type. Also disclosed are methods for modifying the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the same cell. | 2017-12-28 |
20170369911 | OLEAGINOUS YEAST VARIANT, METHOD FOR OBTAINING THEREOF AND USE THEREOF FOR LIPID PRODUCTION - The present invention concerns an | 2017-12-28 |
20170369912 | ENHANCED PRODUCTION OF CORE LIPIDS IN OLEAGINOUS YEASTS - Disclosed are transformed cells comprising one or more genetic modifications that increase the lipid content of the cell, e.g., relative to an unmodified cell of the same type. Also disclosed are methods for increasing the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the cell. | 2017-12-28 |
20170369913 | METHOD OF PRODUCING & PROCESSING DIAMINES - Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process. | 2017-12-28 |
20170369914 | METHODS OF PRODUCING 6-CARBON CHEMICALS USING 2,6-DIAMINOPIMELATE AS PRECURSOR TO 2-AMINOPIMELATE - This document describes biochemical pathways for producing 2-aminopimelate from 2,6-diaminopimelate, and methods for converting 2-aminopimelate to one or more of adipic acid, adipate semialdehyde, caprolactam, 6-aminohexanoic acid, 6-hexanoic acid, hexamethylenediamine, or 1,6-hexanediol by decarboxylating 2-aminopimelate into a six carbon chain aliphatic backbone and enzymatically forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in the backbone. | 2017-12-28 |
20170369915 | Production Of Lysergic Acid By Genetic Modification Of A Fungus - The present invention provides a method of producing lysergic acid and other ergot alkaloids by genetic modification of a fungus. A strain of fungus comprising | 2017-12-28 |
20170369916 | MICROORGANISM HAVING ENHANCED CELLULOSE PRODUCTIVITY - A genetically modified microorganism of the genus | 2017-12-28 |
20170369917 | PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS - The invention relates to a process for the preparation of a sugar and/or fermentation product from lignocellulosic material. | 2017-12-28 |
20170369918 | ADJUSTING THE PH OF A PRETREATMENT SOLUTION USING CARBON DIOXIDE USEFUL FOR INTEGRATING SACCHARIFICATION AND FERMENTATION - The present invention provides for a method of fermenting or saccharifying a biomass comprising: (a) (i) contacting a biomass comprising a polysaccharide, and an ionic liquid (IL) to form a first solution, or (ii) providing the first solution comprising the biomass and the IL, (b) contacting the first solution and carbon dioxide such that the first solution results in a lower pH, (c) introducing (i) an enzyme capable of enzymatically to breakdown at least one bond in the polysaccharide or a breakdown product of the polysaccharide, and/or (ii) a microorganism that capable of producing the enzyme and/or fermenting the polysaccharide or a breakdown product of the polysaccharide, such that the polysaccharide is at least partially broken down and the first solution is transformed into a second solution. | 2017-12-28 |
20170369919 | PROCESS FOR THE RAPID HYDROLYSIS OF HIGH SOLIDS BIOMASS - The process for the hydrolysis of ligno-cellulosic biomass comprises the steps of
| 2017-12-28 |
20170369920 | SEPARATION OF 2'-FL FROM A FERMENTATION BROTH - The invention relates to a method for selective crystallization of 2′-FL from an aqueous solution comprising 2′-FL and one or more other fucosylated carbohydrates by adding acetic acid to the solution. | 2017-12-28 |
20170369921 | ANALYSIS OF NUCLEIC ACIDS ASSOCIATED WITH SINGLE CELLS USING NUCLEIC ACID BARCODES - Provided herein are methods and compositions for analyzing nucleic acids associated with single cells using nucleic acid barcodes. According to some embodiments, a method for producing one or more polynucleotides of interest comprises: obtaining a plurality of RNAs associated with one or more samples, wherein the samples are obtained from one or more subjects, each RNA is associated with a single sample, and the RNAs associated with each sample are present in a separate reaction volume; adding an adapter molecule to the RNAs associated with each sample, wherein the adapter molecule is generated using an enzymatic reaction and comprises a universal priming sequence, a barcode sequence, and a binding site; and incorporating the barcode sequence into one or more polynucleotides associated with each sample, thereby producing the one or more polynucleotides of interest. | 2017-12-28 |
20170369922 | PRODUCTION OF STEVIOL GLYCOSIDE IN RECOMBINANT HOSTS - The invention relates to recombinant microorganisms and methods for producing steviol glycosides, glycosylated ent-kaurenol, and glycosylated ent-kaurenoic acid. | 2017-12-28 |
20170369923 | METHODS AND MEANS FOR THE PRODUCTION OF IG-LIKE MOLECULES - The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided. | 2017-12-28 |
20170369924 | METHOD FOR MICROBIAL ENRICHMENT - The present invention is directed to methods for the growth, enrichment and isolation of microbes within a sample for further use in presumptive identification of pathogenic bacteria that may therein be contained. | 2017-12-28 |
20170369925 | Liquid to Liquid Biological Particle Concentrator - A rapid one-pass liquid filtration system efficiently concentrates biological particles that are suspended in liquid from a dilute feed suspension. A sample concentrate or retentate suspension is retained while eliminating the separated fluid in a separate flow stream. Suspended biological particles include such materials as proteins/toxins, viruses, DNA, and/or bacteria in the size range of approximately 0.001 micron to 20 microns diameter. Concentration of these particles is advantageous for detection of target particles in a dilute suspension, because concentrating them into a small volume makes them easier to detect. Additional concentration stages may be added in “cascade” fashion, in order to concentrate particles below the size cut of each preceding stage remaining in the separated fluid in a concentrated sample suspension. This process can also be used to create a “band-pass” concentration for concentration of a particular target size particle within a narrow range. | 2017-12-28 |
20170369926 | NEW MARKERS FOR EARLY DIAGNOSIS OF OVARIAN CANCER, MONITORING DURING THERAPY, AND NEW THERAPY OPTIONS DURING AND AFTER CHEMOTHERAPY - The inventors have identified several proteases and a protease inhibitor that are overexpressed in ovarian cancer tumors. They have developed monoclonal antibodies against the proteins and shown that they can be detected in serum and the levels of the proteins in serum fluctuate during cancer treatment. They have shown that serum assays for the proteases and protease inhibitor can be used for early detection of ovarian cancer, and for monitoring cancer treatment. | 2017-12-28 |
20170369927 | METHOD AND DEVICE FOR QUANTIFICATION OF TARGET MOLECULES - A method for quantifying a plurality of target molecules in a sample may include releasing a target molecule from a non-covalent bond of a conjugate by using a fusion molecule. A kit may include a detection conjugate, a release reagent, nucleic acid amplification agents, and an amplification detection probe. A device may be designed to perform the methods. | 2017-12-28 |
20170369928 | METHOD FOR EVALUATING DNA DAMAGE FROM ANALYTE - The present disclosure provides a method for evaluating DNA damage by an analyte and a method for screening a DNA damage inhibitor. According to the present invention, the present invention can quantitatively evaluate the extent of DNA damage by an analyte through visualization. | 2017-12-28 |
20170369929 | COMPOSITION FOR REDUCING INHIBITION OF NUCLEIC ACID AMPLIFICATION - A composition for reducing the inhibitory effects of contaminants on nucleic acid amplification is provided. The composition includes effective amounts of ferric iron, an organic iron-chelating reagent, and a non-ionic surfactant. Optionally, the composition includes polyvinylpyrrolidone. The composition has a pH of about 8.45 to 8.85. The organic iron-chelating reagent has a first affinity constant greater than or equal to 10 | 2017-12-28 |
20170369930 | METHOD FOR IDENTIFYING PATHOGENS OF BACTERIAL INFECTIOUS DISEASES BY USING BACTERIA-DERIVED NANOVESICLES - The present invention relates to a method for predicting causative factors (bacteria) of serious bacterial infectious diseases in a clinical sample containing bacteria-derived nano-sized extracellular vesicles, that is, nanovesicles, through the analysis of the genes contained in the nanovesicles. According to the present invention, information on bacteria of serious bacterial infection can be provided, a causative factor of the bacterial infection can be predicted, and the resistance of the bacteria to antibiotics can be predicted. | 2017-12-28 |
20170369931 | COMPOSITIONS AND METHODS FOR DETECTING ZIKA VIRUS - The invention is directed to compositions, kits, and methods for amplifying and detecting a Zika virus nucleic acid sequence in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes. | 2017-12-28 |
20170369932 | METHODS AND KITS FOR DETECTING CELL-FREE PATHOGEN-SPECIFIC NUCLEIC ACIDS - The present invention relates to a method for detecting a target nucleic acid derived from a pathogen in a subject. The method comprises (a) amplifying the nucleic acid sequence of the target nucleic acid, which is obtained from a cell-free fraction of a blood sample from the subject, to produce a double stranded DNA is produced, and (b) detecting the double stranded DNA. The presence of the double stranded DNA indicates the presence of the target nucleic acid in the subject. Also provided are kits for detecting a target nucleic acid derived from a pathogen in a subject. | 2017-12-28 |
20170369933 | METHOD AND SYSTEM FOR MICROBIOME-DERIVED DIAGNOSTICS AND THERAPEUTICS FOR CONDITIONS ASSOCIATED WITH MICROBIOME FUNCTIONAL FEATURES - A method for at least one of characterizing, diagnosing, and treating a condition associated with microbiome functional features in at least a subject, the method comprising: receiving an aggregate set of biological samples from a population of subjects; generating at least one of a microbiome composition dataset and a microbiome functional diversity dataset for the population of subjects; generating a characterization of the condition based upon features extracted from at least one of the microbiome composition dataset and the microbiome functional diversity dataset; based upon the characterization, generating a therapy model configured to correct the condition; and at an output device associated with the subject, promoting a therapy to the subject based upon the characterization and the therapy model. | 2017-12-28 |
20170369934 | METHOD FOR RAPID DESIGN OF VALID HIGH-QUALITY PRIMERS AND PROBES FOR MULTIPLE TARGET GENES IN QPCR EXPERIMENTS - Disclosed is a method of designing a valid primer pair satisfying a specificity condition. The method includes searching for an identifier of a base sequence from a genetic information index based on a query language associated with a gene, searching for a candidate primer from a provided candidate primer set index to satisfy the specificity condition based on the identifier of the base sequence, filtering the candidate primer based on primer-related filtering conditions, and providing information about a primer pair satisfying the query language and the filtering conditions based on a result of the filtering. | 2017-12-28 |
20170369935 | NUCLEIC ACID-BASED LINKERS FOR DETECTING AND MEASURING INTERACTIONS - The invention provides compositions comprising nucleic acid complexes for use in monitoring binding interactions and in measuring association and/or dissociation kinetics, detecting analytes including low concentration analytes, and screening library members. In some instances, the nucleic acid complexes are double-stranded nicked nucleic acids comprising a scaffold nucleic acid hybridized to one or more oligonucleotides. In some instances, a first, a second, a third, and optionally a fourth oligonucleotide are linked to moieties that are known to interact with each other or which are suspected of interacting with each other or of interacting with a common moiety such as an analyte. Changes in topology of the complex are used to determine the binding interactions of the various binding partners. | 2017-12-28 |
20170369936 | MICROARRAY SYSTEM WITH IMPROVED SEQUENCE SPECIFICITY - The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times. | 2017-12-28 |
20170369937 | HiC: Method of Identifying Interactions Between Genomic Loci - The disclosed Hi-C protocol can identify genomic loci that are spatially co-located in vivo. These spatial co-locations may include, but are not limited to, intrachromosomal interactions and/or interchromosomal interactions. Hi-C techniques may be applied to many different scales of interest. For example, on a large scale, Hi-C techniques can be used to identify long-range interactions between distant genomic loci. | 2017-12-28 |
20170369938 | DEVICE AND METHOD FOR PRODUCING A REPLICATE OR DERIVATIVE FROM AN ARRAY OF MOLECULES, AND APPLICATIONS THEREOF - A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array. | 2017-12-28 |
20170369939 | Rapid Quantitative Detection of Single Nucleotide Polymorphisms or Somatic Variants and Methods to Identify Malignant Neoplasms - Provided are systems, kits, and methods for the quantitative detection of single nucleotide polymorphisms or variants to identify malignant neoplasms. The methods include use of modified oligonucleotide blockers with peptide nucleic acid backbones that hybridize to and block logarithmic amplification of the wild-type alleles of a target, and incorporation of locked nucleic acids into probes that are complementary to a mutant allele of the target sequence to increase specificity. The methods include detection of variants in sequences with high GC content and/or low complexity, such as the TERT promoter, IDH1, BRAF, NRAS, GNAQ, GNA11 and H3F3 A gene variants. The methods include sensitive detection and staging of cancers with low cellularity, and can be used intraoperatively such as for glioma, or to detect cell-free circulating tumor DNA, such as for melanoma. | 2017-12-28 |
20170369940 | PRIMER SET FOR DETECTION OF ZAIRE EBOLA VIRUS, ASSAY KIT, AND AMPLIFICATION METHOD - According to one embodiment, there is provided a nucleic acid primer set that amplifies a ZEBOV gene. An F1 sequence includes at least 13 consecutive bases included in SEQ ID NO: 31 or 64. An F2 sequence includes at least 13 bases included in SEQ ID NO: 62 or 63. An F3 sequence includes at least 13 bases included in SEQ ID NO: 29, 36, 38, 55, 56, 57, 58, 59, 60, 61 or 61. A B1c sequence includes at least 13 bases included in SEQ ID NO: 68, 69, 70, 71, 72, 73, 74 or 75. A B2c sequence includes at least 13 bases included in SEQ ID NO: 65 or 66. A B3c sequence includes at least 13 bases included in SEQ ID NO: 34, 67, 82 or 83. | 2017-12-28 |
20170369941 | METHOD AND PRODUCT - The present invention relates to a method which prevents undesirable binding of ddNTPs to double stranded polynucleotides when in the presence of a polymerase. Such methods may be used to prevent the appearance of false positives in methods employing ddNTPs, e.g. in sequence detection methods. The present invention also provides a method of avoiding a false Tm reading or false FRET effects (such as false positive quenching), for example in a melting curve analysis method. In particular a method is provided in which a target nucleotide sequence in a test polynucleotide is detected using a method in which a double stranded molecule is generated which may or may not comprise two labels depending on whether the target sequence is present in which the presence of the two labels is determined, preferably by performing a melting curve analysis. | 2017-12-28 |
20170369942 | COMPOSITIONS, SYSTEMS, AND METHODS FOR DETECTING EVENTS USING TETHERS ANCHORED TO OR ADJACENT TO NANOPORES - Compositions, systems, and methods for detecting events are provided. A composition can include a nanopore including a first side, a second side, and an aperture extending through the first and second sides; and a permanent tether including head and tail regions and an elongated body disposed therebetween. The head region can be anchored to or adjacent to the first or second side of the nanopore. The elongated body including a reporter region can be movable within the aperture responsive to a first event occurring adjacent to the first side of the nanopore. For example, the reporter region is translationally movable toward the first side responsive to the first event, then toward the second side, then toward the first side responsive to a second event. The first event can include adding a first nucleotide to a polynucleotide. The second event can include adding a second nucleotide to the polynucleotide | 2017-12-28 |
20170369943 | METHOD AND DEVICE FOR NUCLEIC ACID SEQUENCING - A method for sequencing a nucleic acid strand, comprising the steps of: providing a solution containing truncated strands having lengths different from one another terminating with a respective dideoxynucleotide from among ddATP, ddTTP, ddGTP, and ddCTP; functionalizing first masses by a donor molecule and second masses by an acceptor molecule such as to generate a light emission when they come into mutual contact; coupling a first mass to a first end of each truncated strand; coupling the second masses to a respective terminal dideoxynucleotide of each strand; applying an AC electrical field having variable frequencies that are such as to generate, on each second mass, a net movement directed towards the first mass; acquiring a plurality of light radiations for each frequency value; and associating each light radiation acquired to a respective dideoxynucleotide and, thus, to a respective nucleotide base. | 2017-12-28 |
20170369944 | COUNTERACTING OSMOTIC IMBALANCE IN A SEQUENCING CELL - A method of analyzing a molecule is disclosed. A lipid bilayer is formed such that it divides a first reservoir characterized by a first reservoir osmolarity from a second reservoir characterized by a second reservoir osmolarity. An electrolyte solution is flowed to the first reservoir that tends to make a first change to a ratio of the first reservoir osmolarity to the second reservoir osmolarity. A voltage is applied across the lipid bilayer, wherein the lipid bilayer is inserted with a nanopore, and wherein a net transfer of ions between the first reservoir and the second reservoir tends to make a second change to the ratio of the first reservoir osmolarity to the second reservoir osmolarity, and wherein the first change to the ratio and the second change to the ratio tends to counter-balance each other. | 2017-12-28 |
20170369945 | METHODS OF DIAGNOSING AUTISM SPECTRUM DISORDERS - Methods of screening subjects for genetic markers associated with autism spectrum disorders are disclosed. In particular, the invention relates to methods of diagnosing autism spectrum disorders by detecting the presence of deleterious mutations or aberrant expression of genes associated with autism spectrum disorders. The present invention relates to genetic markers associated with ASD and methods of screening subjects for such genetic markers. In particular, the invention relates to methods of diagnosing ASD by detecting the presence of deleterious mutations or aberrant expression of genes associated with ASD. | 2017-12-28 |
20170369946 | BLOOD BIOMARKERS FOR RESPIRATORY INFECTIONS - Methods and kits for diagnosing and/or treating a lower respiratory infection in a subject include obtaining a biological sample from the subject; detecting RNA expression levels of one or more biomarkers in the biological sample and comparing the expression levels of the one or more three biomarkers to at least one invariant control marker wherein an increase or decrease in the level of expression of the one or more biomarkers as compared to the at least one invariant control marker is indicative of a lower respiratory infection. | 2017-12-28 |
20170369947 | KIT AND METHOD FOR DETECTING MUTATIONS IN CTNNB1 AND HTERT, AND USE THEREOF IN HCC DETECTION AND DISEASE MANAGEMENT - Provided herein are a kit and method for detecting mutations in CTNNB1 and hTERT, and their use in detection and management of hepatocellular carcinoma (HCC). The kit comprises a first pair of primers, configured to specifically bind sequences flanking the genomic region for amplifying the genomic region in a first PCR reaction; and at least one clamp, each configured to bind to one first allele but not any second allele at an annealing temperature in the first PCR reaction to thereby selectively suppress amplification of the one first allele but still allow amplification of other second allele(s). Kits and methods for detecting mutations in CTNNB1 and hTERT are also provided. A method for detecting or monitoring recurrence of HCC is further disclosed, which comprises determining levels of five DNA markers, including CTNNB1 mutations, hTERT mutations, TP53 mutations, RASSF1A methylation, and GSTP1 methylation. | 2017-12-28 |
20170369948 | METHODS AND COMPOSITIONS FOR DETECTING COLORECTAL NEOPLASIAS - The disclosure provides methods for identifying genomic loci that are differentially methylated in colorectal neoplasias. Identification of methylated genomic loci has numerous uses, including for example, to characterize disease risk, to predict responsiveness to therapy, to non-invasively diagnose subjects and to treat subjects determined to have colorectal neoplasias. | 2017-12-28 |
20170369949 | POLYMORPHISM BIOMARKERS PREDICT CLINICAL OUTCOMES OF CANCER PATIENTS RECEIVING REGORAFENIB - Methods are provided for determining whether a cancer patient is likely or not likely to experience HFSR (hand foot skin reaction) from a therapy comprising administration of an effective amount of regorafenib or an equivalent thereof, the methods including screening a biological sample isolated from the patient for the rs2280789 polymorphism, and/or the rs3817655 polymorphism. Methods are further provided for identifying the clinical outcome of cancer patients following a therapy comprising regorafenib or an equivalent thereof, the methods entailing screening a biological sample isolated from the patient for the rs7527092 polymorphism, and/or rs2071559 polymorphism. After determining if a patient is likely to be successfully treated, the disclosure also provides methods for treating the patient. | 2017-12-28 |
20170369950 | Biomarkers of Response to Inhibition of Poly-ADP Ribose Polymerase (PARP) in Cancer - Provided herein are methods of identifying a subject having a poly-ADP ribose polymerase (PARP) inhibitor-sensitive tumor by detecting a genomic gain in chromosome 1q21 and/or chromosome 20q13.3 in a tumor sample from the subject. Also provided are methods of identifying a subject having a PARP inhibitor-sensitive tumor by detecting gene amplification of a CHD1L gene or an RTEL1 gene in a tumor sample from the subject. Further provided are methods of treating a tumor with a genomic gain in chromosome 1q21 and/or chromosome 20q13.3 in a subject by administering an effective dose of a PARP inhibitor. | 2017-12-28 |
20170369951 | METHOD FOR IDENTIFICATION OF ANTI-HIV HUMAN MIRNA MIMICS AND MIRNA INHIBITORS AND ANTI-HIV PHARMACEUTICAL COMPOUNDS - The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection. | 2017-12-28 |
20170369952 | METHODS AND COMPOSITIONS FOR IDENTIFYING AND QUANTIFYING MICROBIAL DNA - This invention relates to methods and compositions for identifying microbial DNA in the tissues or body fluid samples of patients. More particularly, the invention relates to two-step polymerase chain reaction based methods for identifying microbial DNA in the tissues or body fluid samples of patients, and compositions therefor. Microbial DNA can also be quantified using the methods described herein. | 2017-12-28 |
20170369953 | MAIZE EVENT DP-004114-3 AND METHODS FOR DETECTION THEREOF - The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided. | 2017-12-28 |
20170369954 | MONOAZO DYES WITH CYCLIC AMINE AS FLUORESCENCE QUENCHERS - The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties. | 2017-12-28 |
20170369955 | ORGANO-CATALYTIC BIOMASS DECONSTRUCTION - The present invention provides processes for catalytic deconstruction of biomass using a solvent produced in a bioreforming reaction. | 2017-12-28 |
20170369956 | PROCESS FOR THE PRODUCTION OF SOLID SACCHARIDES FROM AN AQUEOUS SACCHARIDE SOLUTION - Solid saccharides are produced from an aqueous saccharide solution in a process, where the aqueous saccharide solution is admixed with a carrier liquid in which the saccharides are insoluble and that has a boiling point higher than that of water to obtain an aqueous admixture, and where the aqueous admixture is subjected to an evaporation step. The heat for the evaporation is at least partially supplied by a heated surface area to yield a vapor fraction including water and a residue fraction including solid saccharides and carrier liquid. | 2017-12-28 |
20170369957 | METHODS FOR EXTRACTING AND CONVERTING HEMICELLULOSE SUGARS - The present disclosure relates to systems, methods, and processes for the production of sugars and conversion products from biomass. | 2017-12-28 |
20170369958 | THICK-WALLED HIGH-TOUGHNESS HIGH-STRENGTH STEEL PLATE AND METHOD FOR MANUFACTURING THE SAME - A thick-walled, high-toughness, high-strength steel plate manufactured from steel having a particular composition and casted under conditions where the cooling rate of a surface during solidification is 1° C./s or less. The surface of the steel plate has a toughness (vE-40) of 70 J or more, and the steel plate has a thickness of 100 mm or more. | 2017-12-28 |