52nd week of 2015 patent applcation highlights part 26 |
Patent application number | Title | Published |
20150368632 | CRYSTALLINE MATERIALS ON BIOLOGICAL TISSUE AND METHODS FOR MAKING THE SAME - Provided are compositions including a direct interface between a biological tissue and a crystalline material, wherein the crystalline material has a crystallization temperature that exceeds the temperature at which the biological tissue incurs thermal damage. Also provided are methods for producing said compositions. The ability to apply crystalline materials to biological tissues has wide applicability, e.g., with cyborg applications. Extensive challenges exist, however, in applying crystalline materials to biological tissue substrates. | 2015-12-24 |
20150368633 | Pharmaceutical Composition for Elevating Radiation-Sensitivity of Cancer Cells, Pharmaceutical Composition for Detecting Cancer Cells with Radiation-Sensitivity, and Detection Method Thereof - The present invention relates to a pharmaceutical composition for elevating radiation-sensitivity of cancer cells, which comprises: a nanoparticle containing with a first element, which is iron, copper, or the combination thereof; and a pharmaceutically acceptable carrier, wherein the nanoparticle is a metal nanoparticle, an alloy nanoparticle, or a metal nanoparticle with core-shell structure. In addition, the present invention provides a detection method to detect radiation-sensitivity of the cancer cells through different modalities such as CT or MRI due to its native high CT number and magnetic property. Furthermore, the present invention provides a pharmaceutical composition for elevating radiation-sensitivity of the cancer cells through preferential uptake of the nanoparticle, in order to enhance the radiation-sensitivity of the cancer cells and improve the efficiency of radiation therapy to the cancer cells. | 2015-12-24 |
20150368634 | METHOD AND APPARATUS FOR EXTRACTING DNA FROM A BIOLOGICAL SAMPLE - A method of extracting DNA from a biological sample includes the steps of freezing a biological sample; mechanically breaking down the sample to produce a processed sample; mixing the processed sample with a lysis solution; and cycling the mixture through a series of pressure fluctuations to produce a resulting lysate. The method may further include the steps of exposing the lysate to a material that binds to DNA present in the sample and collecting the DNA from the sample using an elution solution. An apparatus for practicing the method includes a first segment for mechanically breaking down the sample; a second segment for pressurizing a mixture of the processed sample and lysis solution to produce a lysate; and wherein the first segment is fluidly coupled to the second segment. The apparatus may also include a third segment in which the lysate is exposed to the binding material. | 2015-12-24 |
20150368635 | MICROFLUIDIC DEVICES TO EXTRACT, CONCENTRATE AND ISOLATE MOLECULES - Methods, systems, and devices are disclosed for capturing, concentrating, and isolating molecules in a fluid. In one aspect, a device includes a substrate formed of a material that is electrically insulating, a microfluidic channel made of an electrically insulating material formed on the substrate to carry a biofluid containing molecules including nucleic acids, an array of electrodes formed on the surface along a parallel direction of the microfluidic channel constituting a capture region, in which the array of electrodes are operable to produce an electric field across the microfluidic channel that creates an electrostatic attractive force on the nucleic acids to immobilize them in the capture region, and a chamber formed on the substrate of the electrically insulating material and connected to the microfluidic channel, the chamber configured to have a volume less than that of the microfluidic channel, in which, when the nucleic acids are released from immobilization in the capture region, the released nucleic acids are collected in the chamber. | 2015-12-24 |
20150368636 | TREATMENT OF A SAMPLE VESSEL - Provided herein is technology related to the chemical modification and purification of DNA. Specifically, the technology provides methods for performing a bisulfite conversion reaction on small amounts of single-stranded, fragmented DNA and performing the subsequent desulfonation and purification steps on magnetic beads, and methods for treating sample vessels prior to recovery of converted DNA from the vessel. | 2015-12-24 |
20150368637 | ACCELERATED DIRECTED EVOLUTION OF MICROBIAL CONSORTIA FOR THE DEVELOPMENT OF DESIRABLE PLANT PHENOTYPIC TRAITS - The disclosure relates to methods for the screening, identification, and/or application of one or more microorganisms of use in imparting one or more beneficial properties to one or more plants. | 2015-12-24 |
20150368638 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID SEQUENCING - Embodiments of the present invention relate to sequencing nucleic acids. In particular, embodiments of the methods and compositions provided herein relate to preparing nucleic acid templates and obtaining sequence data therefrom. | 2015-12-24 |
20150368639 | COMPOSITIONS, METHODS AND USES FOR MULTIPLEX PROTEIN SEQUENCE ACTIVITY RELATIONSHIP MAPPING - Embodiments herein concern systems, compositions, methods and uses for in vivo selection of optimum target proteins of use in designing genomically-engineered cells or organisms. Some embodiments relate to compositions and methods for generating barcoded constructs of use in systems and methods described. | 2015-12-24 |
20150368640 | Novel shRNA Molecules and Methods of Use Thereof - The present invention relates to certain novel shRNA molecules and methods of use thereof. According to certain embodiments of the present invention, methods for reducing the expression level of a target gene are provided. Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. A first RNA molecule comprises a double stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. In addition, a second RNA molecule comprises a second double stranded sequence, which includes a second guide strand sequence that is partially complementary to a portion of the mRNA transcript encoded by the target gene. Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene. | 2015-12-24 |
20150368641 | Methods to screen compounds for regulating USF1 activity and methods and compounds to treat cardiometabolic and lipid pathologies - The deficiency of Usf1 confers a remarkable number of clinically relevant beneficial metabolic effects in mice via activation of brown adipose tissue. The Usf1 deficient mice have high serum HDL-cholesterol and low triglyceride levels, a beneficial lipid profile opposite to that of human metabolic syndrome. The elevated HDL-C is associated with enhanced cholesterol efflux, and low triglycerides with decreased hepatic VLDL production and elevated triglyceride clearance. Despite their elevated food intake and lower physical activity, the Usf1 deficient mice are protected against diet-induced obesity. Their concomitant increase in energy expenditure is related to the activation of brown adipose tissue. The protective effects of Usf1 deficiency against obesity, insulin resistance, fatty liver, dyslipidemia, vascular inflammation, and atherosclerosis coupled with brown adipose tissue activation are demonstrated. Inhibition or silencing of USF1 is suggested as a therapeutic target to treat various human diseases. | 2015-12-24 |
20150368642 | LNA OLIGONUCLEOTIDE CARBOHYDRATE CONJUGATES - The invention provides LNA therapeutics oligonucleotide carbohydrate conjugates with considerably enhanced potency, extended therapeutic index and reduced toxicity. | 2015-12-24 |
20150368643 | OLIGONUCLEOTIDES COMPRISING ALTERNATING SEGMENTS AND USES THEREOF - The invention relates to oligonucleotides having alternating segments of sugar-modified nucleosides and 2′-deoxynucleosides, and uses thereof. The invention further related to oligonucleotides having alternating segments of sugar-modified nucleotides and 2′-deoxynucleotides, and uses thereof. Such uses include the preparation of antisense oligonucleotides and their use for the prevention or depletion of function of a target nucleic acid of interest, such as an RNA, in a system. Accordingly, and oligonucleotide of the invention is useful for therapeutic, analytical and diagnostic methods and uses, as well as component of compositions and commercial packages corresponding to such methods and uses. | 2015-12-24 |
20150368644 | TREATMENT OF INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1 (IFRD1) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO IFRD1 - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Interferon-related tested developmental regulator 1 (IFRD1), in particular, by targeting natural antisense polynucleotides of interferon-related developmental regulator 1 (IFRD1). The invention also relates to the identification of these antisense oligonucleotides and their use in framing diseases and disorders associated with the expression of IFRD1. | 2015-12-24 |
20150368645 | CELL-TYPE SPECIFIC APTAMER-siRNA DELIVERY SYSTEM FOR HIV-1 THERAPY - The present invention relates to compositions and methods for delivery of siRNA to specific cells or tissue. More particularly, the present invention relates to compositions and methods for cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer. | 2015-12-24 |
20150368646 | COMPOSITIONS AND METHODS FOR TREATING PANCREATIC CANCER - The present disclosure provides compositions and methods for treating or reducing the risk of pancreatic cancer by administering compounds capable of inhibiting the expression or activity of RUNX3. | 2015-12-24 |
20150368647 | DIAGNOSIS AND TREATMENT OF CANCERS WITH MICRORNA LOCATED IN OR NEAR CANCER-ASSOCIATED CHROMOSOMAL FEATURES - MicroRNA genes are highly associated with chromosomal features involved in the etiology of different cancers. The perturbations in the genomic structure or chromosomal architecture of a cell caused by these cancer-associated chromosomal features can affect the expression of the miR gene(s) located in close proximity to that chromosomal feature. Evaluation of miR gene expression can therefore be used to indicate the presence of a cancer-causing chromosomal lesion in a subject. As the change in miR gene expression level caused by a cancer-associated chromosomal feature may also contribute to cancerigenesis, a given cancer can be treated by restoring the level of miR gene expression to normal. microRNA expression profiling can be used to diagnose cancer and predict whether a particular cancer is associated with an adverse prognosis. The identification of specific mutations associated with genomic regions that harbor miR genes in CLL patients provides a means for diagnosing CLL and possibly other cancers. | 2015-12-24 |
20150368648 | COMPOSITIONS AND METHODS OF INHIBITING GENE EXPRESSION IN A LUNG - The present invention provides compositions and methods for delivery to a lung tissue comprising a small interfering RNA (siRNA) capable of inhibiting expression of a gene, and a surfactant. In one aspect, a non-polymeric methods composition comprising a small interfering RNA (siRNA) capable of inhibiting expression of a gene, and a surfactant is disclosed. In other aspects, a method of inhibiting gene expression in a lung of a subject in need thereof and treating bronchopulmonary dysplasia in a lung of a subject also disclosed. The methods comprise administering a therapeutically effective amount of a non-polymeric composition to the lung of the subject, wherein the non-polymeric composition comprises a small interfering RNA (siRNA) capable of inhibiting expression of a gene, and a surfactant. | 2015-12-24 |
20150368649 | GENETIC CONTROL METHOD AND COMPOSITIONS AGAINST INSECT PESTS IN COTTON PLANTS BY THE SILENCING OF GENES OF THE LACCASE FAMILY - The present invention relates to the control of infestation of pests by inhibiting or reducing the expression of genes of the family of chitin synthase. The invention further provides methods and compositions for controlling pests by feeding them with one or more double-strand RNA molecules provides by the present invention. The invention further describes a method of obtaining transgenic plants that express double-strand RNA molecules. The present invention is preferably used for cotton-plants. | 2015-12-24 |
20150368650 | USE OF VEGFR1 AS A BIOMARKER - The present invention is related to the use of VEGFR1 or of a nucleic acid coding for VEGFR1 as a biomarker in a method for the treatment of a subject, wherein the method for the treatment comprises administering to the subject a PKN3 inhibitor. | 2015-12-24 |
20150368651 | FURIN-KNOCKDOWN BI-FUNCTIONAL RNA - Compositions and methods to attenuate the immunosuppressive activity of TGF-β through the use of bi-functional shRNAs is described herein. The bi-functional shRNAs of the present invention knocks down the expression of furin in cancer cells to augment tumor antigen expression, presentation, and processing through expression of the GM-CSF transgene. | 2015-12-24 |
20150368652 | TREATMENT OF NEUROPATHIC PAIN - The present invention relates to the field of neurology. More specifically, the present invention provides methods and composition useful for treating neuropathic pain. In a specific embodiment, the present invention provides a recombinant Kcna2 sense fragment. In another embodiment, the present invention provides a method of treating neuropathic pain comprising the step of administering a composition comprising a recombinant Kcna2 sense fragment. | 2015-12-24 |
20150368653 | ANTISENSE MODULATION OF NUCLEAR HORMONE RECEPTORS - Provided are antisense oligonucleotides and other agents that target and modulate nuclear hormone receptors (NHRs) such as the glucocorticoid receptor (GR), compositions that comprise the same, and methods of use thereof. | 2015-12-24 |
20150368654 | MODIFIED LISTERIA BACTERIA AND USES THEREOF | 2015-12-24 |
20150368655 | TRANSFORMED EUGLENA AND PROCESS FOR PRODUCING SAME | 2015-12-24 |
20150368656 | INCREASED PRODUCTION OF ISOBUTANOL IN YEAST WITH REDUCED MITOCHONDRIAL AMINO ACID BIOSYNTHESIS - Yeast cells with reduced activity of certain enzymes involved in branched chain amino acid biosynthesis in yeast mitochondria are described. Target enzymes include threonine deaminase, isopropylmalate synthase, and optionally branched chain amino acid transaminase. | 2015-12-24 |
20150368657 | MATERIAL AND METHODS TO INCREASE PLANT GROWTH AND YIELD - The present invention relates to materials and methods for modulating growth rates, yield, and/or resistance to drought conditions in plants. In one embodiment, a method of the invention comprises increasing expression of an hc1 gene (or a homolog thereof that provides for substantially the same activity), or increasing expression or activity of the protein encoded by an hc1 gene thereof, in a plant, wherein expression of the hc1 gene or expression or activity of the protein encoded by an hc1 gene results in increased growth rate, yield, and/or drought resistance in the plant. | 2015-12-24 |
20150368658 | AGROBACTERIUM HAVING HIGHLY EFFICIENT GENE TRANSFER ABILITY TO PLANT IMPARTED THERETO - The present invention relates to an | 2015-12-24 |
20150368659 | POTATOES WITH REDUCED GRANULE-BOUND STARCH SYNTHASE - Materials and methods for making plants (e.g., | 2015-12-24 |
20150368660 | GEMINIVIRAL VECTOR FOR EXPRESSION OF RITUXIMAB - A single vector or multiple separate vectors that contain two or more non-competing replicons for transient expression of the heavy and light chains of Rituximab in | 2015-12-24 |
20150368661 | METHODS FOR PRODUCING ANTIBODIES - Methods for producing antibodies are provided and include transforming a plant cell with a nucleic acid encoding a heavy chain of an antibody, a light chain of an antibody, and a linking polypeptide connecting the heavy chain to the light chain. The nucleic acid is then expressed in the plant cell, such that, upon expression the linking polypeptide is cleaved to separate the heavy chain from the light chain. Isolated nucleic acid sequences, expression vectors, and transformed plant cells useful for producing the antibodies are also provided. Further provided are methods for treating a viral infection and include the steps of obtaining an antibody produced by the above-described methods and then administering the antibody to a subject. | 2015-12-24 |
20150368662 | Tobacco Having Altered Amounts of Environmental Contaminants and Methods for Producing Such Lines - Disclosed herein are genetically modified tobacco plants, and tobacco products derived from such plants, comprising an altered uptake and/or altered levels of at least one environmental contaminant or other non-natural chemical as compared to an unmodified parent tobacco plant from which the modified plant is derived. The alteration in uptake and/or levels may be a reduction, or alternatively an increase, in the uptake and/or levels of the environmental contaminant or other non-natural chemical. These methods and plants are useful for improving tobacco products or in generating tobacco lines that may have environmentally useful properties. | 2015-12-24 |
20150368663 | METHOD FOR ISOLATION OF TRANSCRIPTION TERMINATION SEQUENCES - The invention relates to efficient, high-throughput methods, systems, and DNA constructs for identification and isolation of transcription termination sequences. The invention relates further to specific terminator sequences identified by said methods isolated from rice. | 2015-12-24 |
20150368664 | PLANTS OF THE GENUS DIPLOTAXIS HAVING CYTOPLASMIC MALE STERILITY - The present invention concerns plants, seeds and cells of the genus | 2015-12-24 |
20150368665 | MODIFIED POLYNUCLEOTIDES FOR THE PRODUCTION OF SECRETED PROTEINS - The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules. | 2015-12-24 |
20150368666 | DESIGNER CIRCUIT CONTROLLING DIET-INDUCED OBESITY - The invention relates to vectors and mammalian cells in a system useful for switching on or switching off gene expression in response to fatty acids, and a method of treating diet-induced obesity. In particular, a synthetic intracellular lipid-sensing receptor was constructed that constantly monitors blood fatty acid levels, processes diet-associated hyperlipidemia and coordinates reversible and adjustable expression of the anorectic peptide hormone pramlintide to reduce dietary intake, blood fat levels and body weight. | 2015-12-24 |
20150368667 | COMPOSITIONS AND METHODS FOR TREATING TYPE 1 AND TYPE 2 DIABETES AND RELATED DISORDERS - The invention features compositions comprising in vitro generated beta cells capable of glucose-stimulated insulin secretion, methods of inducing beta cell maturation from embryonic or induced pluripotent stem cell-derived beta-like cells, and methods of using in vitro generated beta cells for the treatment of type 1 diabetes, type 2 diabetes, or a related disorder. | 2015-12-24 |
20150368668 | HCV E2 CONSTRUCT COMPOSITIONS AND METHODS - Methods of producing and using Hepatitis C virus (HCV) eE2 polypeptides are described. | 2015-12-24 |
20150368669 | GENE DELIVERY SYSTEM CONTAINING RELAXIN GENE AND PHARMACEUTICAL COMPOSITION USING RELAXIN - The present invention relates to a novel gene delivery system and recombinant adenovirus including the relaxin-encoding sequence to enhance transduction efficiency of transgenes, a pharmaceutical anti-tumor composition including the recombinant adenovirus, a pharmaceutical composition having improved tissue penetration potency and a pharmaceutical composition for treating a disease or disorder associated with accumulation of excess extracellular matrix. | 2015-12-24 |
20150368670 | COMPOSITIONS AND METHODS OF DELIVERING TREATMENTS FOR LATENT VIRAL INFECTIONS - The invention provides delivery methods and compositions for antiviral therapeutics. Methods and compositions are provided for targeted delivery of antiviral therapeutics into cells of interest using, for example, viral vectors such as adenovirus, AAV, and replication incompetent HSV. These and other delivery systems can be used as vehicles to deliver DNA vectors encoding a nuclease or a cell-killing gene. These delivery methods can also be used to deliver naked DNA or RNA, protein products, plasmids containing a promoter that is active only in a latent viral state which drives a cell-killing gene, or other therapeutic agents. | 2015-12-24 |
20150368671 | Vault and Vault like Carrier Molecules - A method of using vaults as carrier molecules to deliver one or more than one substance to an organism, or to a specific tissue or to specific cells, or to an environmental medium. A vault-like particle. A method of preventing damage by one or more than one substance to an organism, to a specific tissue, to specific cells, or to an environmental medium, by sequestering the one or more than one substance within a vault-like particle. A method of delivering one or more than one substance or a sensor to an organism, to a specific tissue, to specific cells, or to an environmental medium. According to another embodiment of the present invention, there is provided a method of making vault-like particles, and making vault-like particles comprising one or more than one substance, or one or more than one sensor. | 2015-12-24 |
20150368672 | NUCLEOTIDE VACCINE - The present invention relates to a vaccine comprising a nucleic acid construct such as a DNA construct especially a nucleic acid construct comprising sequences encoding invariant chain operatively linked to antigenic protein or peptide encoding sequences. The vaccine stimulates an immune response, especially an immune response in an MHC-I dependent, but CD4 | 2015-12-24 |
20150368673 | PRODUCTION OF TRICHODERMA CITRINOVIRIDE MICROPROPAGULES AS A BIOCONTROL AGENT BY MEANS OF AN ECONOMICAL PROCESS - The present invention relates to producing micropropagules from | 2015-12-24 |
20150368674 | ENHANCING D-XYLOSE AND L-ARABINOSE UTILIZATION IN ZYMOMONAS CELLS - Disrupting expression of a protein encoded by the region of the | 2015-12-24 |
20150368675 | Ethanol Production in Microorganisms - The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium. | 2015-12-24 |
20150368676 | COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS - The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO. | 2015-12-24 |
20150368677 | RECOMBINANT CELL, AND METHOD FOR PRODUCING 1,4-BUTANEDIOL - An object of the present invention is to provide a series of techniques for producing 1,4-butanediol from methanol or the like. Provided is a recombinant cell prepared by introducing a gene encoding at least one enzyme selected from the group consisting of succinate semialdehyde dehydrogenase, succinyl-CoA synthase, CoA-dependent succinate semialdehyde dehydrogenase, 4-hydroxybutyrate dehydrogenase, 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA reductase, 4-hydroxybutyraldehyde dehydrogenase, and alcohol dehydrogenase, into a host cell which is a methylotroph, wherein the gene is expressed in the host cell, and the recombinant cell is capable of producing 1,4-butanediol from at least one C1 compound selected from the group consisting of methane, methanol, methylamine, formic acid, formaldehyde, and formamide. | 2015-12-24 |
20150368678 | PRODUCTION OF BUTANOL - The present invention relates to methods of making solvents, such as butanol, acetone or ethanol. In particular, the invention relates to a process for producing a solvent, comprising the step of culturing a solventogenic | 2015-12-24 |
20150368679 | METHODS OF PITCHING YEAST FOR FERMENTATION, AND RELATED METHODS OF FERMENTATION AND SYSTEMS - The present invention relates to methods and systems of pitching yeast to fermentation reactors. More particularly, the present invention involves pitching yeast from one fermentation tank to at least one additional fermentation tank. Advantageously, yeast can be continuously pitched from fermentor to fermentor for as long as practically desirable. | 2015-12-24 |
20150368680 | PROCESS FOR THE SEQUESTRATION OF CARBON DIOXIDE AND THE FERMENTATIVE PRODUCTION OF ORGANIC COMPOUNDS - A process is described for the use of biological components of anaerobic microorganisms, in particular cultures or enzymes of anaerobic bacteria from the order Thermotogales such as | 2015-12-24 |
20150368681 | Dehydroascorbic Acid Process - Processes to produce dehydroascorbic acid at the time of use are provided, so that this unstable form of vitamin C can be conveniently utilized for dietary purposes. Products produced by these processes are also described. | 2015-12-24 |
20150368682 | IMMOBILIZED TRANSAMINASES AND PROCESS FOR MAKING AND USING IMMOBILIZED TRANSAMINASE - The invention is directed to immobilized transaminases and methods of making and using them. | 2015-12-24 |
20150368683 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2015-12-24 |
20150368684 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as energy, fuels, foods or materials. Two or more sugars can be produced and these can be further processed and purified. For example, a mixture of the two or more sugars can be selectively fermented to leave one or more sugars in the mixture along with a product. The unfermented sugar may be fermented with a different fermenting system and produce a second product. | 2015-12-24 |
20150368685 | Methods and compositions for degrading cellulosic material - The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material. | 2015-12-24 |
20150368686 | METHOD FOR PREPARATIVE PRODUCTION OF LONG NUCLEIC ACIDS BY PCR - The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method. | 2015-12-24 |
20150368687 | Compositions and Methods for Synthesis of High Fidelity Oligonucleotides - Aspects of the invention relate to methods, compositions for synthesizing high fidelity oligonucleotides. | 2015-12-24 |
20150368688 | METHOD OF MAKING AN ELECTROCHEMICAL SENSOR STRIP - A method of making an electrochemical sensor strip that includes: depositing a first electrode on a base; depositing a second electrode on the base; applying a first layer onto the first electrode; and applying a second layer onto the second electrode. The first layer includes an oxidoreductase and a mediator. The second layer includes a soluble redox species. | 2015-12-24 |
20150368689 | VIABILITY STAINING METHOD - The invention relates to a method of detecting viable cells in a cell sample, using a membrane permeable fluorescent label that permeates both viable and non-viable cells and a membrane impermeant quencher that selectively permeates non-viable cells. | 2015-12-24 |
20150368690 | VIABILITY STAINING METHOD - The invention relates to a method of detecting viable cells in a cell sample, using a membrane permeable fluorescent label that permeates both viable and non-viable cells and a membrane impermeant quencher that selectively permeates non-viable cells. | 2015-12-24 |
20150368691 | One-step Rapid Assay for the Detection of Inhibitors of Enzymes - A device and method for the rapid on-site detection of inhibitors of enzymes, such as acetylcholinesterase, is described wherein the device contains all reagents added to a sample pad containing dried releasable enzyme creating a reaction mixture wherein inhibitor deactivates the enzyme, while said reaction mixture travels via a longitudinal membrane to a distal porous pad containing a substrate for the enzyme. The reaction of the enzyme and the substrate results in a product that can generate a measurable signal such as color, fluorescence or luminescence to serve as a reporter. Signal that is generated at this reaction zone is inversely proportional to inhibitor concentration in the test sample. A device containing two such strips, one for a test sample, the other for a negative control fluid as an onboard comparator is described. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter. | 2015-12-24 |
20150368692 | ANALYSIS OF THE ESTERIFICATION LEVEL OF CEREBROSTEROL AS A TOOL FOR DIAGNOSIS AND/OR PROGNOSIS OF NEURODEGENERATION - The present invention relates to a method for diagnosis and/or prognosis or for monitoring the activity of the enzyme LCAT in the central nervous system, comprising the steps of measuring the amount of non-esterified cerebrosterol ([24(S)OH—C | 2015-12-24 |
20150368693 | METHODS FOR GENERATING STABILIZED LYOPHILIZED MATERIALS - Lyophilized biological reagents, such as enzymes (e.g., PCR reagents) and antibodies, are provided that include a wax component. Thus, in some aspects, a method is provided for storing a biological reagent comprising formulating the reagent into a lyophilized composition including a wax component. Methods for using such lyophilized reagents are likewise provided. | 2015-12-24 |
20150368694 | METHODS FOR CLOSED CHROMATIN MAPPING AND DNA METHYLATION ANALYSIS FOR SINGLE CELLS - Methods of identifying DNase I Hyper-Resistant Sites (DHRS), or in board sense, highly compact chromatin and characterizing the DNA methylation status of DMRs such as CpG islands and CpG island shores are provided. The methods are particularly useful for analysis of genomic DNA from low quantities of cells, for example, less than 1,000 cells, less than 100 cells, less than 10 cells, or even one cell, and can be used to generate chromatin and methylation profiles. The downstream analyses include in parallel massive sequencing, microarray, PCR and Sanger sequencing, hybridization and other platforms. These methods can be used to generate chromatin and DNA methylation profiles in drug development, diagnostics, and therapeutic applications are also provided. | 2015-12-24 |
20150368695 | HOST-ASSOCIATED DNA SEQUENCES, PRIMERS, AND PROBES FOR PCR-BASED IDENTIFICATION OF DOG FECAL POLLUTION SOURCES - A method for detecting dog-fecal contamination in a sample, comprising assaying the sample using a nucleotide sequence based genetic assay which comprises contacting the sample with at least one nucleic acid molecule having the nucleic acid sequence shown in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or 11, the nucleic acid sequence being capable of binding to a nucleic acid sequence in the sample, and detecting binding of the nucleic acid molecule to the nucleic acid sequence in the sample, wherein a presence of binding is indicative of the presence of dog-fecal contamination in the sample; the nucleic acid molecules; and a kit comprising at least two of the above-described nucleic acid molecules. | 2015-12-24 |
20150368696 | GENE-MATCHED ENRICHMENT AND POLYMERASE CHAIN REACTION FOR RAPID DETECTION OF MICROORGANISMS - A method for amplifying and detecting microorganisms, such as species of | 2015-12-24 |
20150368697 | ON-SLIDE STAINING BY PRIMER EXTENSION - A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided. | 2015-12-24 |
20150368698 | PROCESSES FOR DETECTING OR QUANTIFYING NUCLEIC ACIDS USING AN ARRAY OF FIXED OR IMMOBILIZED NUCLEIC ACIDS - This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention. | 2015-12-24 |
20150368699 | PROCESSES FOR DETECTING OR QUANTIFYING NUCLEIC ACIDS USING AN ARRAY OF FIXED OR IMMOBILIZED NUCLEIC ACIDS - This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention. | 2015-12-24 |
20150368700 | PROCESS FOR DETECTING OR QUANTIFYING NUCLEIC ACIDS IN A LIBRARY - This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention. | 2015-12-24 |
20150368701 | Methods And Kits For Room Temperature In Situ Detection Of A Target Nucleic Acid in a Biological Sample - The present invention relates to in situ hybridization methods for detecting a target nucleic acid in a biological sample comprising performing one or more method steps (e.g., pretreatment, denaturation, hybridization, washes) at room temperature. The invention further relates to kits for performing such methods. | 2015-12-24 |
20150368702 | Systems and Methods for Calibration Using Dye Signal Amplification - The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for | 2015-12-24 |
20150368703 | PRIMER TECHNOLOGY - The present invention provides a method of nucleic acid manipulation comprising: (i) hybridizing a double stranded primer to the 3′ end of a single stranded nucleic acid template, wherein said primer comprises a double stranded region and a single stranded region, wherein the single stranded region is a 3′ overhang region and wherein the 3′ overhang region enables the double stranded primer to target and hybridize to the 3′ end of the nucleic acid template, wherein said single stranded 3′ overhang region comprises a degenerate sequence or a sequence comprising universal bases, and wherein the double stranded primer is made up of two separate strands; (ii) at least one round of polymerization using a polypeptide with 5′ to 3′ DNA polymerization activity, wherein at least the first round of polymerization comprises using a polypeptide with 5′ to 3′ DNA polymerization activity to carry out a primer extension reaction to synthesise nucleotides in a template dependent manner from the 3′ end of the single stranded region of said hybridized double stranded primer; wherein the hybridizing step (i) and at least the first primer extension reaction from the 3′ end of the single stranded region of said hybridized double stranded primer in step (ii) takes place without the formation of a phosphodiester bond between the 3′ end of the nucleic acid template and the double stranded primer of step (i). Products, kits and compositions suitable for use in such methods are also provided. | 2015-12-24 |
20150368704 | METHODS AND COMPOSITIONS FOR SINGLE CELL GENOMICS - Presented are methods and compositions for obtaining sequence information from one or more individual cells. The methods are useful for obtaining sequence information for a single nucleotide sequence, and for multiplex generation of sequence information from one or more individual cells. | 2015-12-24 |
20150368705 | METHOD FOR SEQUENCING A TEMPLATE NUCLEIC ACID IMMOBILIZED ON A SUBSTRATE - The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired. | 2015-12-24 |
20150368706 | METHODS FOR SINGLE-MOLECULE ANALYSIS - Methods for single-molecule preparation and analysis are disclosed herein. The methods can, for example, be used for isolating and analyzing DNA from various biological samples. | 2015-12-24 |
20150368707 | SYSTEMS AND METHODS FOR AUTOMATED REUSABLE PARALLEL BIOLOGICAL REACTIONS - A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions. | 2015-12-24 |
20150368708 | SYSTEMS AND METHODS TO DETECT RARE MUTATIONS AND COPY NUMBER VARIATION - The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease. | 2015-12-24 |
20150368709 | Method and Kit for Detecting a Wild-Type and/or a Mutated Target DNA Sequence - The present invention relates to a method for detecting a first and/or a second target DNA sequence from a DNA library, differing in that a mutation generates/eliminates a restriction site for a restriction endonuclease, comprising the steps of: (a) providing the DNA library, in which each of the DNA sequences comprises a first sequence segment, a second sequence segment of genomic DNA as cleaved by the restriction endonuclease, and a third sequence segment reverse complementary to the union of said first sequence segment and | 2015-12-24 |
20150368710 | CHEMICAL METHODS FOR PRODUCING TAGGED NUCLEOTIDES - This disclosure provides systems and methods for attaching nanopore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disclosed tagged nucleotides. | 2015-12-24 |
20150368711 | METHOD FOR HIGH THROUGHPUT SCREENING OF NUCLEIC ACIDS - A scalable reaction and detection system for automated high throughput sequencing of nucleic acids involving a combination of chemical processes and observation processes independent of the chemistry processes. Discrete functional units may be configured in a manner that allows the system to interchangeably utilize different sequencing reaction components in conjunction with discrete apparatus components for optical image collection and/or analysis. | 2015-12-24 |
20150368712 | UNIVERSAL NUCLEIC ACID PROBE SET AND METHOD FOR UTILIZATION THEREOF - A nucleic acid probe set includes (A) a fluorescent probe and (B) a binding probe. The fluorescent probe (A) is formed of an oligonucleotide, which includes (a) a nucleotide unit labeled with (d) a fluorescent substance. The binding probe (B) is formed of an oligonucleotide having (b | 2015-12-24 |
20150368713 | METHOD FOR GENERATING RETINAL PIGMENT EPITHELIUM (RPE) CELLS FROM INDUCED PLURIPOTENT STEM CELLS (IPSCs) - High efficiency methods for producing retinal pigment epithelial cells from induced pluripotent stem cells (iPSCs) are disclosed herein. The iPSCs are produced from somatic cells, including retinal pigment epithelial (RPE) cells, such as fetal RPE stem cells. In some embodiments, the iPSC include a tyrosinase promoter operably linked to a marker. Methods are disclosed for using the RPE cells, such as for treatment. Methods for screening for agents that affect RPE differentiation are also disclosed. | 2015-12-24 |
20150368714 | Method of Testing for Endometriosis and Treatment Therefor - The present invention relates to novel genetic markers associated with endometriosis and risk of developing endometriosis, and methods and materials for determining whether a human subject has endometriosis or is at risk of developing endometriosis and the use of such risk information in selectively administering a treatment that at least partially prevents or compensates for an endometriosis related symptom. | 2015-12-24 |
20150368715 | DIAGNOSTIC AND TREATMENT OF CHRONIC HEART FAILURE - The invention relates to Apolipoprotein O (Apo O) as a biomarker for chronic heart failure. Moreover, the invention relates to a compound which may be an inhibitor of Apo O activity or an inhibitor of the Apo O gene expression for use in the treatment of chronic heart failure. | 2015-12-24 |
20150368716 | METHOD FOR MEASURING BONE LOSS RATE - The present invention relates to a method for diagnosing bone loss rate, particularly in the field of bone anchored implants. The present patent provides with a method that comprises the steps of: a) quantifying the expression level of one or more markers or ratio thereof related to the activity of osteoclasts and/or osteoblasts in an ex vivo sample; and b) determining the bone loss rate as a function of ongoing loss of marginal bone level by interpolating the value obtained in step a) in one or more calibration curves. The invention also relates to a kit for performing said method. | 2015-12-24 |
20150368717 | SYSTEMS AND METHODS FOR MULTI-ANALYSIS - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 2015-12-24 |
20150368718 | METHOD OF TREATMENT BASED ON POLYMORPHISMS OF THE KCNQ1 GENE - The invention provides methods for the administration of compounds capable of prolonging a QTc interval and methods for predicting whether an individual is predisposed to such QTc prolongation. | 2015-12-24 |
20150368719 | DENDRITIC CELL RESPONSE GENE EXPRESSION, COMPOSITIONS OF MATTERS AND METHODS OF USE THEREOF - This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences dendritic cell (DC) response(s), for example, dendritic cell maturation, dendritic cell antiviral response(s) and/or dendritic cell inflammatory response(s), as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences dendritic cell response(s) in a variety of therapeutic and/or diagnostic indications. | 2015-12-24 |
20150368720 | METHODS AND COMPOSITIONS OF PREDICTING ACTIVITY OF RETINOID X RECEPTOR MODULATOR - The present invention describes genomic biomarkers that have been discovered to correlate with varied individual responses (efficacy, adverse effect, and other end points) to therapeutic retinoid X receptor modulator, such as bexarotene, in treating diseases such as, non small cell lung cancer. The newly discovered biomarkers and others in linkage disequilibrium with them can be used in companion diagnostic tests which can help to predict drug responses and apply drugs only to those who will be benefited, or exclude those who might have adverse effects, by the treatment. | 2015-12-24 |
20150368721 | INDEX OF GENOMIC EXPRESSION OF ESTROGEN RECEPTOR (ER) AND ER-RELATED GENES - The present invention provides the identification and combination of genes that are expressed in tumors that are responsive to a given therapeutic agent and whose combined expression can be used as an index that correlates with responsiveness to that therapeutic agent. One or more of the genes of the present invention may be used as markers (or surrogate markers) to identify tumors that are likely to be successfully treated by that agent or class of agents such as hormonal or endocrine therapy or chemotherapy. | 2015-12-24 |
20150368722 | MAKER FOR DIAGNOSING HER2 INHIBITOR RESISTANT CANCER, DIAGNOSTIC KIT COMPRISING SAME, AND METHOD FOR DIAGNOSING HER2 INHIBITOR RESISTANT CANCER - The present invention relates to a composition for detecting a marker for diagnosing an HER2 inhibitor-resistant cancer, a diagnostic kit including same, and a method for detecting the marker. More particularly, the present invention relates to a composition for detecting a maker for diagnosing an HER2 inhibitor-resistant cancer, a diagnostic kit including same, and a method for detecting the marker, wherein the present invention allows presence and absence of resistance to an HER2 inhibitor, which is typically prescribed to an HER2-positive cancer patient, in an HER2-positive cancer patient to be determined in an easier manner with remarkably high reliability. | 2015-12-24 |
20150368723 | Identification of Surface-Associated Antigens for Tumor Diagnosis and Therapy - The invention relates to identifying tumor-associated genetic products and encoding nucleic acids thereof. A therapy and diagnosis of diseases in which the tumor-associated genetic products are aberrantly expressed, proteins, polypeptides and peptides which are expressed in association with tumor and the encoding nucleic acids for said proteins, polypeptides and peptides are also disclosed. | 2015-12-24 |
20150368724 | METHODS AND MATERIALS FOR CLASSIFICATION OF TISSUE OF ORIGIN OF TUMOR SAMPLES - The present invention provides a process for classification of cancers and tissues of origin through the analysis of the expression patterns of specific microRNAs and nucleic acid molecules relating thereto. Classification according to a microRNA tree-based expression framework allows optimization of treatment, and determination of specific therapy. | 2015-12-24 |
20150368725 | DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN HUMAN KRAS - Disclosed herein are primers and probes for the detection of single nucleotide polymorphisms (SNPs) in the KRAS gene. These primers and probes may be used in a method of identifying the presence or absence of one or more SNPs in the KRAS gene. These primers and probes may also be used in a method of predicting a response of a subject in need thereof to a cancer therapy. The primers and probes may further be used in a method of selecting a cancer therapy for a subject in need thereof. | 2015-12-24 |
20150368726 | NUCLEOPHOSMIN PROTEIN (NPM) MUTANTS, CORRESPONDING GENE SEQUENCES AND USES THEREOF - The invention relates to new nucleophosmin protein (NPM) mutants, corresponding gene sequences and relative uses thereof for diagnosis, monitoring of minimal residual disease, prognostic evaluation and therapy of acute myeloid leukaemia (AML). | 2015-12-24 |
20150368727 | NUCLEIC ACID AMPLIFICATION CONTROLS - The present invention discloses positive control material for nucleic acid amplification based detection of microorganisms in biological samples. The control material comprises purified microorganism that is rendered non-infectious but is amenable to nucleic acid amplification. Also disclosed is a process for making and using the control material. | 2015-12-24 |
20150368728 | DENGUE VIRUS ASSAY - Nucleic acid assays for detecting nucleic acids of Dengue virus serotypes 1-4. | 2015-12-24 |
20150368729 | METHOD FOR THE PRODUCTION OF HIGH-WEAR-RESISTANCE MARTENSITIC CAST STEEL AND STEEL WITH SAID CHARACTERISTICS - The invention relates to a method for the production of martensitic cast steel of high strength and excellent abrasion- and impact-wear resistance, intended for large parts used as anti-wear cladding in crushing and grinding mining operations, having a chemical composition, expressed in percentage by weight, of between 0.35˜0.55% C, 0.60˜1.30% Si, 0.60˜1.40% Mn, 4.5˜6.50% Cr, 0.0˜0.60% Ni, 0.30˜0.60% Mo, 0.0˜0.70% Cu, 0.010˜0.10% Al, 0.0˜0.10% Ti, 0.0˜0.10% Zr, 0.0˜0.050% Nb, less than 0.035% P, less than 0.035% S, less than 0.030% N, optionally 0.0005˜0.005% B, optionally 0.015˜0.080% rare earths, and the rest being iron. The method for the production of cast steel includes smelting, pouring and heat treatment. The smelting can be performed in an electric arc furnace with acidic or basic refractory or an electric induction furnace. Smelting in an electric arc furnace as a normal operation includes melting, oxygen insufflation, blocking, refining and deoxidation. Smelting in an electric induction furnace includes melting, refining, control of nitrogen in solution and deoxidation. The heat treatment comprises hardening in forced or still air depending on the thickness of the parts, followed by a tempering heat treatment. The cast steel of the invention demonstrates excellent resistance to abrasion-/impact-wear and a suitable chemical composition balance, with the addition of microalloying agents in order to obtain high hardenability and full curing in large cast parts, typically up to 14 inches thick, with Brinell hardness preferably around 630 BHN depending on the heat treatment conditions applied. | 2015-12-24 |
20150368730 | METHOD OF TREATMENT OF A RUNNING FERROUS ALLOY SHEET AND TREATMENT LINE FOR ITS IMPLEMENTATION - A method of treatment of a running ferrous alloy sheet containing at least one easily oxidized element is provided. The method includes a step of immersion of the sheet in a molten oxides bath. | 2015-12-24 |
20150368731 | OIL QUENCHING HEAT TREATMENT FURNACE TRAY - A purpose is to provide a tray for oil quenching heat treatment furnaces that can inhibit oil infiltration to reduce adverse effects on the quality of the workpiece and also can make lightweight design of the tray possible. | 2015-12-24 |