50th week of 2010 patent applcation highlights part 38 |
Patent application number | Title | Published |
20100317025 | Diagnostic Method for Celiac Sprue - Detection of toxic gluten oligopeptides refractory to digestion and antibodies and T cells responsive thereto can be used to diagnose Celiac Sprue. | 2010-12-16 |
20100317026 | VMP-Like Sequences Of Pathogenic Borrelia - The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic | 2010-12-16 |
20100317027 | SULFENIC ACID-REACTIVE COMPOUNDS AND THEIR METHODS OF SYNTHESIS AND USE IN DETECTION OR ISOLATION OF SULFENIC ACID-CONTAINING COMPOUNDS - The present invention provides compounds of Formula I: | 2010-12-16 |
20100317028 | BIOASSAY METHOD FOR YOKUKANSAN - The invention intends to find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, and provides a bioassay method for yokukansan, comprising competitively reacting labeled ligand and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring binding activity of yokukansan from the amount of the labeled ligand bound, and a bioassay method for yokukansan, comprising reacting labeled GTP and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring receptor-agonist activity of yokukansan from the amount of the labeled GTP bound. | 2010-12-16 |
20100317029 | Nasopharyngeal cancer malingancy biomarker and method thereof - The present invention discloses a nasopharyngeal cancer malignancy biomarker and a method thereof, wherein relative TP expression is used to evaluate the malignancy of nasopharyngeal cancer. The biomarker of the present invention assists the currently-existing inspections to find out cancer in the early stage and achieve early diagnosis and early therapy. The present invention also functions as an effective indicator to monitor the metastasis and relapse of nasopharyngeal cancer. | 2010-12-16 |
20100317030 | METHODS AND COMPOSITIONS FOR DETERMINING ENZYMATIC ACTIVITY - The present invention comprises crystalline polyketide synthases, isolated non-native polyketide synthases having the structural coordinates of said crystalline polyketide synthases, and nucleic acids encoding such non-native polyketide synthases. Also disclosed are methods of predicting the activity and/or substrated specificity of putative polyketide synthase, methods of identifying potential polyketide synthase substrates, and methods of identifying potential polyketide synthase inhibitors. | 2010-12-16 |
20100317031 | ULTRA-SENSITIVE CHEMILUMINESCENT SUBSTRATES FOR ENZYMES AND THEIR CONJUGATES - New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP. | 2010-12-16 |
20100317032 | METHOD FOR DETECTING ANTIGEN AND ANTIGEN DETECTION DEVICE - Provided is a method for detecting an antigen in a sample, the method including: bringing an unlabeled polypeptide and a labeled polypeptide into contact with an antigen in a sample, the unlabeled polypeptide being one of a pair including a VH-region polypeptide and a VL-region polypeptide which are separate and capable of cooperatively recognizing the antigen, and the labeled polypeptide being the other of the pair including the separate VH-region polypeptide and the VL-region polypeptide and being labeled with an environmentally-responsive substance at a site where the environmentally-responsive substance does not inhibit binding of the antigen, and detecting a change in the environmentally-responsive substance caused by a change in the environment around the labeled polypeptide after the contact. Also provided is an antibody fragment polypeptide set including the unlabeled polypeptide and the labeled polypeptide. | 2010-12-16 |
20100317033 | METHODS AND MATERIALS FOR DETECTING FOOD CONTAINING ALLERGENS - This document provides methods and materials related to detecting allergens (e.g., food allergens) and/or specific antibodies in individualized consumers that are specific to an allergen (e.g., a food allergen). For example, methods and materials for assessing a food sample for the presence or absence of food allergens are provided. In addition, methods and materials for assessing if a particular human will react with those exact food allergens given his/her antibody profile are provided. | 2010-12-16 |
20100317034 | PRIMATE T-LYMPHOTROPIC VIRUSES - Disclosed are compositions and methods related to the isolation and identification of the primate T-lymphotropic viruses, HTLV-3 and HTLV-4. The diversity of HTLVs was investigated among central Africans reporting contact with NHP blood and body fluids through hunting, butchering, and keeping primate pets. Herein it is shown that this population is infected with a variety of HTLVs, including two retroviruses; HTLV-4 is the first member of a novel phylogenetic lineage that is distinct from all known HTLVs and STLVs; HTLV-3 falls within the genetic diversity of STLV-3, a group that has not previously been seen in humans. The present disclosure also relates to vectors and vaccines for use in humans against infection and disease. The disclosure further relates to a variety of bioassays and kits for the detection and diagnosis of infection with and diseases caused by HTLV-3 and HTLV-4 and related viruses. | 2010-12-16 |
20100317035 | USE OF SPECIFIC T2R TASTE RECEPTORS TO IDENTIFY COMPOUNDS THAT BLOCK BITTER TASTE - Assays for identifying compounds that modulate, preferably inhibit bitter taste associated with the activation of hT2R4, hT2R44 and/or hT2R61 are provided. The compounds identified according to these assays should modulate, e.g., inhibit bitter taste associated with bitter tasting compounds including quinine, 6-nitrosaccharin, saccharin and/or denatonium. These compounds are useful additives for foods, beverages or medicinal preparations having a bitter taste. | 2010-12-16 |
20100317036 | Mycobacterium Antigens - There is provided a diagnostic reagent for use in the detection of | 2010-12-16 |
20100317037 | Detection of Mycobacterium Tuberculosis Bacilli - A screening test for | 2010-12-16 |
20100317038 | GRANZYME A AND GRANZYME B DIAGNOSTICS - A method for identifying a subject being at risk for or having a chronic inflammatory disease, fibrillinopathy, atherosclerosis, or coronary artery disease is provided. The method may include determining the concentration of GrA and/or GrB in a blood or serum sample from said subject; and comparing the concentrations to the corresponding concentration in a control sample, wherein an elevated concentration of GrA and/or GrB may be indicative of a chronic inflammatory disease, fibrillinopathy, atherosclerosis, or coronary artery disease. The method may further include identifying concentrations of fibrinogen, elastin and/or fibrillin. | 2010-12-16 |
20100317039 | MOLDING WITH EMBEDDED COUPLING PARTICLES FOR BIOMOLECULES - The invention relates to a molding, comprising a matrix in a material, selected from the group consisting of metal, ceramic and polymer synthetic material, and coupling particles embedded in the matrix, wherein a proportion of the surface of the molding in a geometrical form or in a regular pattern and/or an area of the molding is completely or the entire surface of the molding is mechanically treated. | 2010-12-16 |
20100317040 | METHODS AND KITS FOR DIAGNOSING CANCER - A method of diagnosing oral cancer or oral pharyngeal cancer in a subject in need thereof is provided. The method comprising determining a level or activity of at least one marker in a saliva sample of the subject, wherein a significant alteration in the level or the activity of the marker with respect to an unaffected saliva sample is indicative of the cancer, wherein the saliva marker is selected from the group consisting of Cyclin D1, phospho-Src, 8-oxoguanine DNA glycosylase (OGG1), Maspin, KI67 and translocator protein 18 kDa (TSPO). | 2010-12-16 |
20100317041 | NEURAL PROTEINS AS BIOMARKERS FOR NERVOUS SYSTEM INJURY AND OTHER NEURAL DISORDERS - The present invention identifies biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury. | 2010-12-16 |
20100317042 | PROTEINS ACTIVATING PRO-PHENOLOXIDASE SYSTEM AND GENES ENCODING THE SAME - The present invention provides novel proteins activating pro-phenoloxidase (pro-PO) system of | 2010-12-16 |
20100317043 | MASS SPECTROMETRY ASSAY FOR eIF4E AND eIF4E REGULON ACTIVITY - Provided is a highly sensitive high throughput mass spectrometry-based quantitative assay for 4E/4E regulon pathway proteins has been developed which provides for single sample multiplexed analysis, as well as the analysis of protein phosphorylation states. It may be adapted for use as the first single sample analytical method of the 4E/4E regulon biological pathway. | 2010-12-16 |
20100317044 | MASS SPECTROMETRIC ENDOPEPTIDASE ASSAY - The activity of a selected endopeptidase in a body fluid is determined by the mass spectrometric measurement of the reaction products of reporter substrate molecules added to the body fluid. Each reporter substrate molecule includes a peptide with the cleavage motif of the endopeptidase, an anchor group A1 on one side of the cleavage site and a different anchor group A2 on the other side of the cleavage site. One anchor is used to extract the reporter substrate molecules from the body fluid and the other anchor is used to extract digest fragments of the reporter molecules from the body fluid. Mass markers allow several reporter substrates to be used simultaneously in the same body fluid sample to measure the activity of different types of endopeptidase. | 2010-12-16 |
20100317045 | COMPOUNDS USED AS DYES COMPERABLE TO ALEXA FLUOR 350 DYES - Compounds used as dyes comperable to Alexa Fluor 350 dyes. The inventive compounds have high fluorescence quantum yield and high photostability. The dyes facilitate analysis of biological structures with enhanced sensitivity and selectivity. | 2010-12-16 |
20100317046 | Non-Endogenous, Constitutively Activated Versions of Human G Protein Coupled Receptor: FSHR - The invention disclosed in this patent document relates to transmembrane receptors, particularly to a human G protein-coupled receptor, more particularly to a follicle stimulating hormone receptor (FSHR), and most particularly to mutated (non-endogenous) versions of the human FSHR for evidence of constitutive activity. | 2010-12-16 |
20100317047 | Biomarkers of Hemorrhagic Shock - Methods for the use of claudin-3 as a biomarker for diagnosis and prognosis, and for monitoring the efficacy of treatment, in hemorrhagic shock (HS). | 2010-12-16 |
20100317048 | NOVEL SYNTHETIC BLOCKING REAGENTS - The present invention concerns novel synthetic blocking reagents for the reduction of non-specific bindings in solid phase assays that rely on biological and specific recognition, e.g., in enzyme-linked immunosorbent assays (ELISAs). The invention provides the use of compounds as blocking reagents as well as kits comprising these compounds. The compounds comprise one or more poly(ethylene glycol) moieties, one or more alkyl- or aminoalkyl groups and another unit bridging the aforementioned groups, wherein the compound comprises at least one amino group. | 2010-12-16 |
20100317049 | Stable Cell Lines and Methods for Evaluating Gastrointestinal Absorption of Chemicals - Nucleic acids and vectors for interfering with the expression of membrane efflux transport proteins in cells that express such proteins are provided. Also provided are cells and cell lines comprising such nucleic acids and vectors. Methods for screening chemicals and biomolecules for gastrointestinal absorption in animals, and kits for practicing such methods are also provided. | 2010-12-16 |
20100317050 | POLYPEPTIDES FOR MICROBIAL DETECTION - Polypeptides which can be activated to cause the formation of pores in a lipid membrane are disclosed. Also disclosed are polypeptide compositions for the detection of target microorganisms and methods of using said compositions. | 2010-12-16 |
20100317051 | DEVICE FOR COLLECTING AND TRIGGERED RELEASE OF A BIOLOGICAL SAMPLE - The invention relates to a system for collecting a biological sample comprising a container having at least one open end, a closure fitting on or in said at least one open end, a holding element connected to said closure and a solid matrix on which said biological sample is deposited, and optionally at least one processing agent, wherein said solid matrix is at least partially transferable into a liquid or dissolved state by changing at least one physico-chemical property of the environment of said matrix without disintegration of the biomolecules comprised in said biological sample deposited on said matrix. | 2010-12-16 |
20100317052 | PROCESS FOR DETECTING HELICOBACTER PYLORI USING ALIPHATIC AMIDES - The present invention provides a process for detecting | 2010-12-16 |
20100317053 | PROCESS MACHINERY FOR FEEDING PRE-TREATED LIGNOCELLULOSIC MATERIALS INTO BIOREACTORS FOR BIO-FUELS AND BIOCHEMICALS - Methods for mixing a pretreated cellulosic biomass feedstock using a centrifugal mixer prior to reactions in a bioreactor. | 2010-12-16 |
20100317054 | PORCINE DC-SIGN, ICAM-3 AND LSECtin AND USES THEREOF - The present invention relates to the cloning, identification and characterization of the unique and entire genomic sequences encoding new porcine DC-SIGN and LSECtin proteins, including the novel nucleotide sequences of the full-length cDNA and genes of both pDC-SIGN gene and pLSECtin. Also provided are the nucleic acid molecules encoding newly discovered porcine ICAM-3 isoforms from porcine monocyte-derived dendritic cells and the use thereof. Specifically, the invention is drawn to an isolated nucleic acid molecule comprising a nucleotide sequence encoding one or more of porcine DC-SIGN, porcine ICAM-3, porcine LSECtin, a complement of the nucleotide sequence or a functional, defined portion of the nucleotide sequence or a protein fusion product linked to a protein that may be of porcine or human origin. Methods for isolating and cloning the new porcine genes and for using the new nucleotide sequences in improved methods for propagating viruses, particularly enveloped viruses, are additionally described herein. The invention further includes new transfected cells or cell lines that can stably express the porcine proteins, new antibodies and the like. | 2010-12-16 |
20100317055 | Recombinant Cell Clones Having Increased Stability and Methods of Making and Using the Same - Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones. | 2010-12-16 |
20100317056 | FERMENTATION MEDIAS AND PROCESSES THEREOF - The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as | 2010-12-16 |
20100317057 | SEMI-RECOMBINANT PREPARATION OF GLP-1 ANALOGUES - A semi-recombinant method for the production of GLP-1 analogues and derivatives with non-proteogenic amino acids in the N-terminal part combining the use of recombinant expression techniques and chemical peptide synthesis. | 2010-12-16 |
20100317058 | PROCESS FOR MODIFYING THE THERMAL AND/OR DIGESTION PROPERTIES OF CORN STARCHES AND CORN FLOURS - The present invention relates to processes for modifying the thermal and/or digestion properties of corn starches and corn flours. | 2010-12-16 |
20100317059 | BETA-GLUCOSIDASE VARIANT ENZYMES AND RELATED POLYNUCLEOTIDES - The invention provides variants of the | 2010-12-16 |
20100317060 | METHOD FOR HIGHLY AMPLIFYING TARGET GENE IN MAMMALIAN CELL AND VECTOR THEREFOR - A vector of the present invention is a vector for amplifying a target gene in a mammalian cell, the vector including an amplification-activating fragment, which is a partial fragment of a mammalian replication initiation region and has a gene amplification activity site, and a mammalian nuclear matrix attachment region. In the case where the mammalian replication initiation region as described above derives from a c-myc locus, for example, the above-described partial fragment at least contains a duplex unwinding element and a topoisomerase II-binding domain. The vector as described above improves gene transfer efficiency and gene amplification efficiency compared with the existing high gene amplification systems. Thus, a method whereby a “high gene amplification system” developed by the inventors can amplify a target gene with better gene transfer efficiency and a vector to be used in this method are provided. | 2010-12-16 |
20100317061 | NOVEL DNA CLONING METHOD RELYING ON THE E.COLI recE/recT RECOMBINATION SYSTEM - The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described. | 2010-12-16 |
20100317062 | HOT START REVERSE TRANSCRIPTION BY PRIMER DESIGN - The present teachings provide methods, compositions, and kits for performing primer extension reactions. In some embodiments, a reverse transcription reaction is performed on a target polynucleotide with a hot start primer comprising a blunt-ended self-complementary stem, and a loop, and extension products form at high temperatures but reduce extension product formation at low temperatures. | 2010-12-16 |
20100317063 | COMPOSITIONS FOR IMPROVED cDNA SYNTHESIS - The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention. | 2010-12-16 |
20100317064 | Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase - The present invention provides a method of covalently joining a DNA strand to an RNA strand comprising (a) forming a topoisomerase-DNA intermediate by incubating a DNA cleavage substrate comprising a topoisomerase cleavage site with a topoisomerase specific for that site, wherein the topoisomerase-DNA intermediate has one or more 5′ single-strand tails; and (b) adding to the topoisomerase-DNA intermediate an acceptor RNA strand complementary to the 5′ single-strand tail under conditions permitting a ligation of the covalently bound DNA strand of the topoisomerase-DNA intermediate to the RNA acceptor strand and dissociation of the topoisomerase, thereby covalently joining the DNA strand to the RNA strand. The present invention also provides a method of tagging a 5′ end of an RNA molecule. The present invention further provides a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase. The present invention also provides a method of tagging a 5′ end of an mRNA. The present invention provides a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA. | 2010-12-16 |
20100317065 | GLUCANOTRANSFERASE - The present invention describes an isolated polypeptide which has glucanotransferase activity, selected from the group consisting of: a) a polypeptide which has an amino acid sequence which has at least 40% amino acid sequence identity with amino acids 1 to 555, 1 to 549 or 1 to 567 of SEQ ID NO: 3 or 6 or a fragment thereof; b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO: 1, 2, 4 or 5 which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO: 1, 2, 4 or 5. | 2010-12-16 |
20100317066 | Bioreactor and method for producing microbial cellulose - A technique for producing microbial cellulose is provided, including: preparing a liquid medium for microbial cultivation in a container; horizontally rotating multiple hollow tubes that are fitted together or separated from one another, so that each of the hollow tubes is alternately partially immersed in the liquid medium and partially exposed above the horizontal surface of the liquid medium; wherein each of the hollow tubes has a rough outer surface and a smooth inner surface, so as to allow microorganisms to form microbial cellulose on the outer surface of each hollow tube, as well as forming sheets of microbial cellulose on the horizontal surface of the liquid medium not being disturbed by the hollow tubes, and removing the microbial cellulose from the outer surfaces of the hollow tubes in order to obtain tubular microbial cellulose. In addition, the sheets of microbial cellulose are also harvested from the liquid medium. | 2010-12-16 |
20100317067 | PROMOTER AND A PRODUCTION METHOD FOR L-LYSINE USING THE SAME - Disclosed are a nucleic acid molecule of | 2010-12-16 |
20100317068 | METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME - The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative:
| 2010-12-16 |
20100317069 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF ADIPATE, HEXAMETHYLENEDIAMINE AND 6-AMINOCAPROIC ACID - The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. | 2010-12-16 |
20100317070 | COMPLETE LIQUEFICATION OF LIGNOCELLULOSIC AGROWASTE TO FORM LIQUID BIOFUELS - A process for converting lignocellulosic materials which are field residues such as cotton stalks and corn stover, process residues such as sugarcane bagasse and sweet sorghum bagasse, woody parts of energy crops such as switchgrass and miscanthus, forest residues or byproducts of the wood processing industries such as sawdust from sawmills to a liquid biofuel by a series of processing steps wherein the feed materials are hydrolysed in three stages and withdrawn as three product streams each consisting of solubilized fragments of one of the three major components of the feed materials and a set of concurrently operating processing steps wherein each of the three product streams is transformed through chemical or biochemical processes into products, such as pure lignin and ethanol, that have a high calorific value and process wherein these products with high calorific value are combined to form a liquid biofuel. | 2010-12-16 |
20100317071 | Method for the Enzymatic Production of Fatty Alcohol and/or Fatty Acid - The invention relates to a method for the enzymatic production of C | 2010-12-16 |
20100317072 | OPTIMIZED STRAINS OF YARROWIA LIPOLYTICA FOR HIGH EICOSAPENTAENOIC ACID PRODUCTION - Described are engineered strains of the oleaginous yeast | 2010-12-16 |
20100317073 | MOLECULAR APPROACHES FOR THE OPTIMIZATION OF BIOFUEL PRODUCTION - Embodiments of the present invention utilize rationale genetic and chemical engineering strategies to achieve even greater efficiencies in biofuel production from microalgae. These increased efficiencies may be achieved through the application of targeted and well-designed chemical and genetic engineering methods disclosed herein. The exemplary embodiments focus on increasing single cell oil yields, increased algal culture densities, and increased efficiencies in oil production. Individually or in combination, exemplary embodiments may reduce the cost to produce a barrel of biofuel to enable commercial viability. | 2010-12-16 |
20100317074 | CARBON CAPTURE IN FERMENTATION - The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas. | 2010-12-16 |
20100317075 | Novel Carbonyl Reductase, Gene Thereof and Method of Using the Same - The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus | 2010-12-16 |
20100317076 | STRAIN OF YARROWIA LIPOLYTICA AND ITS USE IN THE INDUSTRIAL RECLAMATION OF GLYCEROL FRACTIONS OBTAINED DURING BIODIESEL PRODUCTION - An industrial method of reclaiming the glycerol fraction resulting from biodiesel production, a new strain of | 2010-12-16 |
20100317077 | Methods for sequestering carbon dioxide into alcohols via gasification fermentation - The present invention is directed to improvements in gasification for use with synthesis gas fermentation. Further, the present invention is directed to improvements in gasification for the production of alcohols from a gaseous substrate containing at least one reducing gas containing at least one microorganism. | 2010-12-16 |
20100317078 | METHODS TO IMPROVE ALCOHOL TOLERANCE OF MICROORGANISMS - The present invention is directed to a method of producing organisms tolerant to alcohol, that includes selecting a microorganism needing tolerance to alcohol and modifying the selected microorganism under conditions effective to overproduce inositol by the microorganism compared to when the microorganism is not modified, with the modified microorganism being tolerant to alcohol. The present invention is also directed to a method of producing alcohol that includes providing a microorganism tolerant to alcohol which is modified to overproduce inositol by the microorganism compared to when the microorganism is not modified. A fermentable feedstock is treated with the modified microorganism under conditions effective to produce the alcohol. The modified microorganism is also able to produce and tolerate alcohol in high osmolarity feedstocks. | 2010-12-16 |
20100317079 | SILAGE WASTE RECOVERY PROCESS, FACILITY, SYSTEM, AND PRODUCT - A silo of conventional construction is provided with a collection pan at its bottom to collect effluent. The effluent can be stored and/or processed. Processing can include separation of water to concentrate the effluent. The effluent can contain fermentation product, such as alcohol, generated within the silo prior to collection, and it can also be fermented after collection. Processed effluent can be handled safely without risk of contamination to ground water at the silo site. | 2010-12-16 |
20100317080 | METHOD FOR THE TREATMENT OF BIOLOGICAL TISSUE OF ANIMAL OR HUMAN ORIGIN, SUCH AS PORCINE, BOVINE PERICARDIUM OR HUMAN CADAVER HEART VALVES, AND BIOLOGICAL TISSUE TREATED ACCORDINGLY - A method for treating glutardialdehyde-stabilised biological tissue of animal or human origin, such as porcine, bovine pericardium or human cadaver heart valves, provides a physical plasma treatment of the, in particular, collagen tissue for increasing the biocompatibility, cell colonisation and durability thereof. | 2010-12-16 |
20100317081 | Counter-Pressure Filtration of Proteins - A method is disclosed for filtering a protein in a liquid mixture in a manner that does not substantially damage or otherwise limit the recovery of the protein in the filtration filtrate. The method generally includes passing a liquid mixture containing a protein (e.g., an aqueous vWF mixture) through a filter while applying a counter pressure to the liquid mixture filtrate to accurately reduce and control the pressure differential across the filter. The disclosed method has the advantage that relatively high filtration flow rates can be achieved at relatively low pressure differentials, in contrast to high pressure differentials, which actually reduce the filtration flow rate of protein liquid mixtures. Further, the method can recover substantially all of the protein that is initially present in the liquid mixture. | 2010-12-16 |
20100317082 | RELATED RIBONUCLEASES IN WHICH AN AMINO ACID SEQUENCE OF A KNOWN RIBONUCLEASE IS PRECEDED BY AT LEAST ONE RESIDUE AT THE N-TERMINUS, AND METHODS OF MAKING THEM RECOMBINANTLY - New RNases, and methods for making them, are disclosed. Each includes an amino acid sequence that is disclosed in U.S. Pat. No. 6,239,257 B1, but that is preceded by a different N-terminus residue or leader sequence. | 2010-12-16 |
20100317083 | METHODS AND KITS FOR DISSOCIATING FCGAMMA-RECEPTOR-IGG COMPLEXES AND FOR IGG PURIFICATION AND DETECTION - The inventions provides methods and kits for the dissociation of Fcγ-receptor-IgG complexes, and methods and kits for the isolation of IgG and Fc and Fab fragments of IgG. | 2010-12-16 |
20100317084 | RECOMBINANT BACTERIA COMPRISING VECTORS FOR EXPRESSION OF NUCLEIC ACID SEQUENCES ENCODING ANTIGENS - The invention encompasses a recombinant bacterium that comprises at least one vector capable of expressing a nucleic acid sequence encoding an antigen. In particular, the bacterium comprises at least one chromosomally encoded essential nucleic acid that is altered so that it is not expressed, and at least one extrachromosomal vector. | 2010-12-16 |
20100317085 | CO-INCUBATING CONFINED MICROBIAL COMMUNITIES - This invention provides devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices. | 2010-12-16 |
20100317086 | LARGE SCALE MICROBIAL CULTURE METHOD - A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having <5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up. | 2010-12-16 |
20100317087 | MODIFIED CELLULASES WITH INCREASED THERMOSTABILITY, THERMOPHILICITY, AND ALKALOPHILICITY - A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme. | 2010-12-16 |
20100317088 | Systems and Methods for Extracting Lipids from Wet Algal Biomass - Presented herein are exemplary systems and methods for extracting lipids from a wet algal biomass. An exemplary method comprises lysing a wet algal biomass with an insoluble granular lysing agent to create a lysate, creating a lipid-rich phase in the lysate, and separating the lipid-rich phase from the lysate. An exemplary system comprises a lysing station for creating a lysate from a wet algal biomass and a separation station for creating and separating a lipid-rich phase from the lysate. According to further exemplary systems and methods, ultrasound may be used in place of or in addition to a lysing agent to lyse the wet algal biomass. | 2010-12-16 |
20100317089 | Production of Volatile Fatty Acids By Means of Mesophilic Fermentation of Sludge - A method and related apparatus for the removal of excess water from sludge, the method comprising the steps of: (1) fermenting the sludge at a temperature in the mesophilic temperature range, (2) maintaining the fermenting sludge for a predetermined period under a hydraulically quiescent condition to achieve phase separation, (3) separately removing the solid phase and liquid phase and (4) feeding the solid phase to the digester for conversion to biogas. | 2010-12-16 |
20100317090 | WASTE TREATMENT SYSTEM - A process for treating mixed waste material, said process comprising a) collecting said mixed waste in bags; b) autoclaving the bags under conditions in which the bags degrade; c) separating the material from step (b) into three fractions, a first fraction comprising liquid materials, a second fraction comprising large solid waste materials and a third fraction comprising fibrous material; d) subjecting the third fraction of step (c) to a remediation process such as an aerobic digestion; and e) recovering the product of step (d). Use of aerobic digestion as a drying process for materials, and certain apparatus for use in the process are also described and claimed. | 2010-12-16 |
20100317091 | BIOGAS APPARATUS AND BIOGAS PRODUCTION PROCESS FOR INTEGRATION WITH AN ETHANOL PRODUCTION SYSTEM AND PROCESS - An integrated system produces ethanol and biogas from raw plant materials. The system includes a pretreatment apparatus for converting raw plant materials into sugars and a fermenter for fermenting the sugars to produce a beer including ethanol. A distillation apparatus separates the beer into the ethanol and a whole stillage, and a separator then separates the whole stillage into a thin stillage and wet distillers grains. A biogas apparatus processes a first portion of the thin stillage to produce biogas and a biogas effluent, and converts a percentage of the non-fermentable solids and organic acids in the thin stillage into biogas. The pretreatment apparatus is supplied with an amount of fresh water and an amount of backset, the backset including the biogas effluent recycled from the biogas apparatus to the pretreatment apparatus. | 2010-12-16 |
20100317092 | COMPONENT MEASURING APPARATUS - A component measuring apparatus is provided with a case having a cylindrical case main body and a cover arranged to cover the base end opening section of the case main body; a chip mounting section for mounting a chip; a light measuring section for detecting a prescribed component; a printed board whereupon a control section having a function of controlling the operation of the light measuring section is arranged; a liquid crystal display device; a battery arranging section for arranging a battery provided on the cover; an O-ring arranged between the case main body and the light measuring section on the leading end section of the case main body; and an O-ring arranged between the case main body and the cover on the base end opening section of the case main body. Sealing of inside the case is ensured by the O-ring. | 2010-12-16 |
20100317093 | FLEXIBLE POUCH AND CARTRIDGE WITH FLUIDIC CIRCUITS - Flexible pouch and flexible cartridge devices for fluid sample processing, related methods of making and using, related manufacturing systems and related instrumentation systems are described. Flexible pouches provide broad advantage in a wide variety of fields by overcoming the need for complex instrumentation, dedicated devices, and relatively high cost in conventional fluid sample devices. Flexible cartridge devices are particularly advantageous in control of fluid handling, rapid adaptation to a number of configurations by the end user, multiple uses for a single configuration, and in cost and ease of manufacture. | 2010-12-16 |
20100317094 | MILK ANALYSIS MICROFLUIDIC APPARATUS FOR DETECTING MASTITIS IN A MILK SAMPLE - A milk analysis apparatus for detecting mastitis in a milk sample by isolating somatic cells in the form of a pellet using centrifugal sedimentation is described. The apparatus comprises a vessel for holding the milk sample, and a centrifuge for rotating the vessel. The vessel includes an inlet for facilitating charging milk into a body portion of the vessel; and a trap for capturing somatic cells suspended in the milk sample. The somatic cells are biased towards the trap upon rotation of the vessel. | 2010-12-16 |
20100317095 | BIOREACTOR - Disclosed is a bioreactor, especially a plug flow bioreactor, including an agitator running along a longitudinal axis for thoroughly mixing a dry solid biomass that is to be outgassed in a fermentation chamber. In said bioreactor, an agitator shaft is mounted exclusively in two opposite end walls of the fermentation chamber while at least one free end of the agitator is connected to a drive unit outside the fermentation chamber. | 2010-12-16 |
20100317096 | Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site - Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self-processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo. | 2010-12-16 |
20100317097 | SYNTHETIC MOLECULES THAT SPECIFICALLY REACT WITH TARGET SEQUENCES - The present invention features biarsenical molecules. Target sequences that specifically react with the biarsenical molecules are also included. The present invention also features kits that include biarsenical molecules and target sequences. Tetraarsenical molecules are also featured in the invention. | 2010-12-16 |
20100317098 | Compositions and Methods for Diagnosing and Treating Cancer - The present invention relates to compositions and methods for characterizing, diagnosing, and treating cancer. In particular the invention provides the means and methods for the diagnosis, characterization, prognosis and treatment of cancer and specifically targeting cancer stem cells. The present invention provides a soluble FZD receptor comprising an extracellular domain of a human FZD receptor that inhibits growth of tumor cells. The present invention still further provides a soluble receptor comprising a Fri domain of a human FZD receptor that binds a ligand of a human FZD receptor and said soluble receptor is capable of inhibiting tumor growth. The present invention still further provides a method of treating cancer comprising administering a soluble FZD receptor comprising for example, either an extracellular domain of a human FZD receptor or a Fri domain of a human FZD receptor, in an amount effective to inhibit tumor growth. | 2010-12-16 |
20100317099 | Liquid Separation from Adipose Tissue - Extracting or removing at least a portion of liquid phase from a whole sample using a centrifugal force is disclosed. Centrifugal forces are used to apply pressure to a whole sample and drive a liquid phase through a passage region that can be perforated and/or porous and maintain a drier portion within a separation container. The whole sample can be dried, which includes a remaining sample where excess or a selected amount of liquid is removed. Direct access to the separation container or area can then be made to provide for an efficient withdrawal of the drier material from the separation container. | 2010-12-16 |
20100317100 | Compositions and methods for establishing and maintaining stem cells in an undifferentiated state - The present invention embraces compositions and methods for establishing and maintaining stem cells and inhibiting stem cell differentiation using a selective Protein Kinase C (PKC) inhibitor. | 2010-12-16 |
20100317101 | Culture System for Rapid Expansion of Human Embryonic Stem Cells - This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy. | 2010-12-16 |
20100317102 | Cell Culture Method and Automatic Culture System Using the Method - A culturing method according to the present invention including a step of injecting cells extracted from a human body and a culture medium into a culture container, (2) a step of culturing cells while performing air ventilation and exchange of the culture medium in the culture container in which the culture medium and the cells are put, (3) a step of cleaning cultured cells which are cultured in the culture container and then withdrawing the cleaned cells from the culture container into a cell withdrawing vessel while some of the cleaned cells are left in the culture container, (4) a step of injecting a culture medium to the cultured cells left in the culture container in the step (3), and (5) a step of repetitively executing the step (2,) and the step (3). | 2010-12-16 |
20100317103 | EFFICIENT GENERATION OF NEURAL PROGENITORS, NEURONS, AND DOPAMINERGIC NEURONS FROM HUMAN EMBRYONIC STEM CELLS - The present invention relates to a method for inducing the differentiation of neural progenitors, neurons, and dopaminergic neurons from human embryonic stem cells with high efficiency, in which neural selection can be performed by the selected media and physical methods. The invention has advantages such as higher efficiency, the effect of lowering cost and time, and maintenance of neural progenitors for a longer period of time, as compared to the known methods for inducing the differentiation into neural progenitors, neurons, and dopaminergic neurons. Accordingly, the method can stably generate cells used for treating Parkinson's disease or other nervous system diseases. | 2010-12-16 |
20100317104 | CELL CULTURE MEDIA - A serum-free media composition for maintenance or directed differentiation of human embryonic stem cells (HESCs) toward particular cell lineages is disclosed. The media comprises one or more cell nutrient media, a recombinant human albumin or equivalent thereof and optionally at least one agent involved in HESC differentiation. The media composition is substantially free of any human or animal derived products. | 2010-12-16 |
20100317105 | Methods and compositions for controlling efficacy of RNA silencing - Based at least in part on an understanding of the mechanisms by which small RNAs (e.g., naturally-occurring miRNAs) mediate RNA silencing in plants, rules have been established for determining, for example, the degree of complementarity required between an RNAi-mediating agent and its target, i.e., whether mismatches are tolerated, the number of mismatches tolerated, the effect of the position of the mismatches, etc. Such rules are useful, in particular, in the design of improved RNAi-mediating agents which allow for more exact control of the efficacy of RNA silencing. | 2010-12-16 |
20100317106 | HARVESTING TARGET MATERIALS FROM CENTRIFUGED SUSPENSIONS - Embodiments of the present invention are directed to harvesting a target material from a suspension using a tube and float system. A suspension suspected of containing a target material is combined with a solution having one or more labels that distinguish the target material from other materials in the suspension. The tube, float, and suspension are centrifuged to separate various materials in the suspension according to associated specific gravities. The float expands the axial length of the target material layer and displaces the target material to a narrow space between the float and the inner wall of the tube. The space is illuminated with light that causes the labels to emit light identifying the location of the target material within the tube. One or more openings can then be formed in the tube at or near the point where the target material is located and the target material harvested. | 2010-12-16 |
20100317107 | METHOD AND DEVICE FOR COLLECTING AND PRESERVING CELLS FOR ANALYSIS - The claimed subject matter comprises a device to collect and preserve cells comprising of: (1) a collection container comprised of a tube having an open end and a closed end, a closure in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and (2) compounds including an anticoagulant agent and a fixative agent, wherein the compounds are in a sufficient amount to preserve said cells” original morphology and antigenic sites without significant dilution of said cells, and thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment. The claimed subject matter further comprises of a method of making a collection device for cells comprising of: (1) providing a tube having an open end and a closed end; (2) preloading compounds including: an anticoagulant agent, and a fixative agent into the tube, wherein the compounds are in a sufficient amount to preserve the cells” original morphology and antigenic sites without significant dilution of the cells, and thereby allowing the cells to be directly analyzed by a flow cytometer without further treatment; (3) inserting a closure into the open end of the tube; and (4) drawing a vacuum inside the tube to a predetermined level to form the collection device. | 2010-12-16 |
20100317108 | Cryopreservation of Biological Cells and Tissues - The method involves placing an oocyte cell in a cell holder ( | 2010-12-16 |
20100317109 | FACTOR - Provided is a method of producing neurite outgrowth and/or development using RARβ2 and/or an agonist thereof. | 2010-12-16 |
20100317110 | CELL-MEDIATED DELIVERY AND TARGETED EROSION OF NONCOVALENTLY CROSSLINKED HYDROGELS - A method for targeted delivery of therapeutic compounds from hydrogels is presented. The method involves administering to a cell a hydrogel in which a therapeutic compound is noncovalently bound to heparin. | 2010-12-16 |
20100317111 | CD40 AGONIST ANTIBODY/TYPE 1 INTERFERON SYNERGISTIC ADJUVANT COMBINATION, CONJUGATES CONTAINING AND USE THEREOF AS A THERAPEUTIC TO ENHANCE CELLULAR IMMUNITY - A synergistic adjuvant is provided comprising synergistically effective amounts of at least one type 1 interferon and at least one CD40 agonist, wherein these moieties may be in the same or separate compositions. In addition, fusion proteins and DNA conjugates which contain a type 1 interferon/CD40 agonist/antigen combination are provided. The use of these compositions, protein and DNA conjugates as immune adjuvants for treatment of various chronic diseases such as HIV infection and for enhancing the efficacy of vaccines (prophylactic and therapeutic) is also provided. | 2010-12-16 |
20100317112 | SCAFFOLDS INCREASED SPECIFIC GRAVITY FOR CELL CULTURE AND METHOD FOR MANUFACTURING THEREOF - The present invention relates to microtype scaffolds for cell culture, which have their specific gravity increased and a method for manufacturing thereof, and more specifically, relates to microtype scaffolds for cell culture, which have their specific gravity increased, by adding a chemically stable inorganic compound having a high specific gravity in manufacturing biocompatible polymer microtype scaffolds for cell culture and a method for manufacturing thereof. In case where the inventive microtype scaffolds for cell culture is used, it is easy to separate cells cultured on microtype scaffolds, and cell damage can be minimized by reducing separation time, and it is easy to recover cells due to a definite boundary layer. | 2010-12-16 |
20100317113 | SYNTHETIC MICROCARRIERS FOR CULTURING CELLS - A cell culture microcarrier includes a polymer formed from copolymerization of a mixture including (i) an uncharged hydrophilic unsaturated monomer having a hydroxyl group; (ii) a hydrophilic carboxylic acid containing unsaturated monomer; and (iii) a hydrophilic multifunctional unsaturated monomer. The microcarrier may further include a polypeptide, such as a polypeptide that promotes cell adhesion, conjugated to the surface of the microcarrier; e.g. via the carboxyl group from the hydrophilic carboxylic acid containing unsaturated monomer. | 2010-12-16 |
20100317114 | METHOD OF GENERATING GLUCOSE-RESPONSIVE CELLS - The present invention provides an improved method for generating cells. The method comprises differentiation of neuronal progenitor cells and transfecting of either already differentiated or progenitor cells to generate certain cells useful for the treatment of an illness such as diabetes. | 2010-12-16 |
20100317115 | METHODS, COMPOSITIONS AND USES FOR ENHANCING CHEMICAL TOLERANCE BY MICROORGANISMS - Embodiments herein concern compositions and methods for enhancing chemical tolerance of biomass conversion by microorganisms. In some embodiments, enhancing tolerance of biomass hydrolysate conversion includes enhancing tolerance to low molecular weight organic compounds. | 2010-12-16 |
20100317116 | SINGLE-MOLECULE REAL-TIME ANALYSIS OF PROTEIN SYNTHESIS - The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of analytical reactions in which protein synthesis is occurring. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance, inhibit, or otherwise affect such reactions including, but not limited to, affecting the reaction rate, processivity, fidelity, duration, and the like. | 2010-12-16 |
20100317117 | SELECTIVE MEMBRANE EXTRACTION OF RADIOACTIVE ANALYTES - A membrane selectively extracted a radioactive analyte from a sample solution. The membrane was prepared as thin film, and includes a soluble extractant and a collodion matrix that disperse the extractant homogeneously throughout the membrane. A membrane was formed by applying a solution including collodion, an extractant, and an organic solvent onto a surface of an aqueous solution containing a radioactive analyte. After the membrane formed on the surface, it was removed and then analyzed. The analysis proved that the membrane extracted radioactive analyte from the solution in far greater amounts compared to a control membrane prepared without the extractant. | 2010-12-16 |
20100317118 | CAPTURING OF CELL FLUID AND ANALYSIS OF ITS COMPONENTS UNDER OBSERVATION OF CELLS AND INSTRUMENTS FOR THE CELL FLUID CAPTURING AND THE ANALYSIS - The invention provide the method to enable the molecular detections of cellular fluid of at least a single cell easily and rapidly and clarify the dynamics of life phenomena and molecular mechanisms very rapidly by conducting simultaneous observations of cellular morphological dynamism. At the same time, it provides the method of exploring active molecules. Even in case that the target is a single cell, the cellular fluid is directly sucked by the capillary tip and the fluid is subjected to the analysis directly. By performing this method under the observation of the cell with image-magnifying scopes, such as a microscope, the cellular fluid is sucked and captured by the nanospray ionization capillary tip even if the target is a single cell and the trapped sample is subjected to the mass spectrometer by nano-spraying the sample solution directly after addition of the ionization supporting solvent into the tip, and then the molecular components in the cellular fluid is detected. The detected molecular peaks specific to a certain state are identified by performing differential analysis or t-test between the data groups of cells in different states. | 2010-12-16 |
20100317119 | Quenched Fluorophores Conjugated to Peptides Via Linkers Containing Dithio Groups - Disclosed are dithio compounds that include a quenched fluorophore and a non-fluorophore peptide linked via a dithio bond to the fluorophore. The dithio compounds may be used in methods for detecting thiol-containing compounds or dithio-containing compounds. The dithio compounds also may be used as cellular probes where the peptide portion of the compounds targets the compounds to a specific cellular location. | 2010-12-16 |
20100317120 | Multiplexed Analyses of Test Samples - The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR). | 2010-12-16 |
20100317121 | PROTEIN SYNTHESIS MONITORING (PSM) - A method and a device are disclosed for monitoring the synthesis of proteins by the ribosome, wherein the ribosome is bound to a first label, for example a donor fluorophore, and a tRNA and/or amino acid are is bound to a second label, for example an acceptor fluorophore, wherein the first and second labels together from a FRET pair. As the ribosome mechanism processes the mRNA and tRNA molecules and synthesizes a polypeptide chain, a light source illuminates the ribosome, exciting the donor fluorophores and thereby the acceptor fluorophores whenever these are in sufficient proximity to a donor. The resulting signals are detected and used as a key for database searching and identification of the protein being synthesized. | 2010-12-16 |
20100317122 | NEW ULTRA-SENSITIVE CHEMILUMINESCENT SUBSTRATES FOR ENZYMES AND THEIR CONJUGATES - New chemiluminescent compounds, stable in aqueous buffers, for use in biological assaying include acridane-based compounds and 1,2-dioxetanes. Among the new acridane-based compounds are water-soluble acridanes, enhancer coupled acridanes, bis and tris-acridanes as well as acridane-1,2-dioxetanes. Among the new 1,2-dioxetanes are electron deficient group-containing dioxetanes and tethered bis-1,2-dioxetanes. The 1,2-dioxetanes are useful as substrates for various enzymes. The acridanes can be admixed with an oxidizing agent. an aqueous buffer and, optionally, a stabilizer to form a substrate or reagent formulation useful for assaying, inter alia, HRP.5 | 2010-12-16 |
20100317123 | Method and Device for Detection of an Analyte - The present invention relates to a method, device and kit for analysing a sample for determining the presence or amount of an analyte, particularly carbohydrate, more particularly sugar, in the sample using a fabric. | 2010-12-16 |
20100317124 | METAL-CONTAINING NANOMEMBRANES FOR MOLECULAR SENSING - A sensor includes a first support having at least one opening; a metal-containing nanomembrane associated with the at least one opening and configured to interact with at least one molecular species; and at least one electrode configured to sense one or more interactions of the at least one molecular species with the metal-containing nanomembrane. | 2010-12-16 |