50th week of 2013 patent applcation highlights part 40 |
Patent application number | Title | Published |
20130330683 | SELF-LOCKING AND POSITIONING DEVICE FOR DENTAL BRACE - A self-locking and positioning device for a dental brace, comprising: a brace main body, an elastic piece, and an upper cover. Wherein, on the brace main body is provided with a track and a fixing slot, while on the elastic piece is provided with a positioning piece and a slot. The positioning piece is provided with a barb, while the upper cover slides and fixes into the brace main body through the track. Then, the barb of the positioning piece on the elastic piece will act against a block portion on bottom of said upper cover, to restrict movement of the upper cover, to lock automatically the upper cover into position. The dental brace is simple in construction and easy to assemble, hereby raising production efficiency and yield. | 2013-12-12 |
20130330684 | MULTIFUNCTION WAND FOR AN INTRA-ORAL IMAGING SYSTEM - A wand having a housing is coupled to an intra-oral imaging system. The wand comprises a first device and a second device coupled to the housing. The first device is an intra-oral imaging device for capturing images of a patient's teeth, and the second device provides an additional function. | 2013-12-12 |
20130330685 | DENTAL SURFACE MODELS - To create a digital surface model of an intra-oral anatomy, a recess or a hole in an impression material having a predetermined shape and size is scanned. The scanning results and information on the predetermined shape and size are used for determining a threshold value representing interface between the impression material and another material. The threshold value is then used in generating the digital surface model from CT scanning data of an impression of an intra-oral anatomy on said impression material. | 2013-12-12 |
20130330686 | DENTAL OPTICAL MEASURING DEVICE AND DENTAL OPTICAL MEASURING/DIAGNOSING TOOL - An OCT measuring mechanism for acquiring the optical coherence tomography information and a surface shape measuring mechanism for acquiring the three-dimensional shape information share a display device, a control device, a light source, a scanning mirror, a light transmission/receiving path and an entering/exiting opening. The scanning mirror, the light transmission/receiving path, the entering/exiting opening are included in a handpiece operable to make a diagnosis on a diagnostic target site. | 2013-12-12 |
20130330687 | INTERFACE STRUCTURE FOR LIQUID/AIR BURST DENTAL CLEANING APPLIANCE - The power dental appliance includes a handle/receiver assembly ( | 2013-12-12 |
20130330688 | METHOD FOR PRODUCING NANOSURFACES WITH NANO, MICRON, AND/OR SUBMICRON STRUCTURES ON A POLYMER - The present invention relates to a modified polymeric material. The modified polymeric material includes a polymer having a modified surface, where the modified surface includes nano, micron, and/or submicron scale features. The present invention also relates to an implant comprising the modified polymeric material. The present invention further relates to processes for making the modified polymeric material and the implant. | 2013-12-12 |
20130330689 | METHODS FOR PRODUCING A LABORATORY ANALOGUE FOR DENTAL IMPLANTS - A method for producing a three-dimensional model of a region of a jaw for producing a dental restoration includes fully-automatic shaping of a recess for accommodating a laboratory analogue into the alveolar ridge of the model. Three-dimensional scanning data is used in the shaping and the depth, position, and orientation of the recess are shaped such that the crestal end of the laboratory analogue inserted into the recess coincides with the crestal end of the dental implant in the jaw of a patient. The method may also include fully-automatic shaping of a vestibular, lingual or palatinal feed-through through the model into the recess, wherein the three-dimensional scanning data are used in the shaping and the feed-through is arranged such that the crestal end of a laboratory analogue coincides with the crestal end of the dental implant in the jaw of the patient, when the laboratory analogue is fixed in place. | 2013-12-12 |
20130330690 | DENTAL VENEER PRODUCT - Certain embodiments relate to a method of forming an individualized dental product ready for application including at least one tooth veneer, which is of a ceramic material. The method includes milling a first side of a ceramic substrate, filling up with a support material against the first side of the substrate, and milling the second side of the substrate until the veneer is plate-like and provided with a bevelled region over a distance (s) taken from an edge and 0.3 mm to 3 mm towards a middle portion of the veneer. The veneer thickness varies from 0 to a plate thickness (d*enlargement factor) over the distance (s). The method further includes sintering the dental product and removing the support material. Certain embodiments also relate to a product resulting from the method. | 2013-12-12 |
20130330691 | SYSTEM FOR EVALUATING DRIVING COMPETENCY OF A SUBJECT - A system for testing and evaluating driving competency of a subject is disclosed. The system includes a first test unit for providing a simple driving reaction test sequence, a complex driving reaction test sequence and a depth perception test sequence, a second test unit for providing a low illumination vision test sequence and a glare recovery test sequence, a third test unit for providing a side vision test sequence, a first switching module for enabling the subject to undergo the simple driving reaction test sequence and the complex driving reaction test sequence, a second switching module for enabling the subject to undergo the depth perception test sequence, the low illumination vision test sequence, and the glare recovery test sequence, a master control unit for interacting and controlling the first test unit, the second test unit, the third test unit, the first switching module, and the second switching module. | 2013-12-12 |
20130330692 | TACTILE DISPLAY AND OPERATING SYSTEM THEREFOR - A tactile display includes a base; movable pins having free ends for tactile sensing by a user, the pins arranged in rows and columns and movable between an extended position and retracted position with respect to the base so as to selectively display tactilely sensible data; a resilient element for supporting each pin in the extended position when at rest, and further operative to urge the pin from the retracted position to the extended position in the presence of an opposing force; one or more reset elements for lowering the pins from the extended position to the retracted position; row stoppers for lockably engaging rows of pins when in the second position. | 2013-12-12 |
20130330693 | Interactive Cognitive Recognition Sports Training System and Methods - According to methods for teaching high performance cognitive skills, a simulated sports action scenario is displayed on a screen to a user, and the user is queried to respond to the scenario. The user's response to the scenario is received. The user's response to the scenario is evaluated according to predetermine high performance cognitive skills criteria to determine a sports relevant score. The determined sports relevant score is then displayed to the user and a database is updated. | 2013-12-12 |
20130330694 | SYSTEM AND METHOD FOR REWARDING PHYSICAL ACTIVITY - A system incentivizes people to engage in physical activity. A person's physical activity may be monitored over a period of time. Such monitoring may occur by using a sensing device carried or worn by the user. In the system, one or more thresholds for physical activity are established. Information tracked using the sensing device can be compared to the thresholds. A percentage of physical activity relative to the threshold can be translated to a reward established for the physical activity, and the percentage may be used to determine a reward value for an electronic device relative to a full available value. Rewards may include times or amounts. Time values may indicate durations during which particular activities or devices may be used. Amount values may indicate an amount of a battery charge, a number of communications, a currency value, or other values relative to use of an electronic device. | 2013-12-12 |
20130330695 | SYSTEM FOR MEDICALLY TREATING A CHILD - A system for medically treating a child comprising a flexible membrane, with indicia thereon, and a plurality of portions, extending distally along the membrane from the start portion. Each portion has at least a second indicia thereon. The second indicia has a characteristic indicative of the developmental age of a child disposed along the membrane. At least one book has pages, each page of the book having the one of the respective at least second indicia thereon. Each page having at least a third indicia corresponding to at least one of a medicine and dosage and the item of medical equipment and size for use with a child of the developmental age indicated by the at least second indicia. | 2013-12-12 |
20130330696 | Rotatable Robotic Manipulation Trainer Assembly - A developmental robotic manipulation trainer assembly may include a housing assembly and a rotatable module carrier assembly for carrying robotic training exercise modules. The rotatable module carrier assembly may rotate about a spindle of the housing assembly. The rotatable module carrier assembly may include a number of dividers for separating the rotatable module carrier assembly into a number of regions. The dividers may be removable from the rotatable module carrier assembly. The developmental robotic manipulation trainer assembly may include a lock member for preventing rotation of the rotatable module carrier assembly. | 2013-12-12 |
20130330697 | Method of Measuring Abdominal Thrusts for Clinical Use and Training - A method and system for determining the adequacy of abdominal thrusts applied to choking victims during performance of the Heimlich maneuver | 2013-12-12 |
20130330698 | SIMULATOR FOR CPR AND DEFIBRILLATOR TRAINING - There is provided a simulator for CPR and defibrillator training, which may perform training and evaluation of CPR and a defibrillator so that when there is a patient in an emergency situation, effective first aid can be provided to the patient. Through the simulator for CPR and defibrillator training, CPR and the use of the defibrillator for emergency medical technicians such as a doctor, a nurse and an emergency medical technician can be systematically and repetitively trained, and a person in charge of training can identify whether or not the education is performed effectively by recording and evaluating a training situation. Also, through the simulator, the education of CPR and defibrillator training is possible so that not only the emergency medical technicians but also general people can perform emergency treatment effectively. | 2013-12-12 |
20130330699 | Interactive Education System for Teaching Patient Care - Simulator systems including a maternal simulator and a fetal simulator are provided. In some instances, the maternal simulator includes a chamber sized and shaped to receive a fetal simulator and a birthing mechanism disposed within the chamber for providing rotational and translational movement to the fetal simulator in a birthing simulation. In some instances, the fetal simulator is configured to be selectively, fixedly engaged with a locking mechanism of the maternal simulator such that the fetal simulator is not removable from the maternal simulator during the birthing simulation when fixedly engaged with the locking mechanism and the fetal simulator is removable from the maternal simulator during birthing simulation when not fixedly engaged with the locking mechanism. | 2013-12-12 |
20130330700 | MODULAR STAGED REALITY SIMULATOR - This invention is directed to a modular staged simulator and a process of simulating medical trauma and maladies for the purpose of training or certifying individuals including medical professionals. More specifically, this disclosure relates to ex vivo training exercises as opposed to traditional forms of training using intact cadavers, in vivo surgery on animal subjects; and supervised apprenticeship performing surgery on human subjects with trauma or maladies under the watch of a skilled medical practitioner. | 2013-12-12 |
20130330701 | PHYSICAL HEART SIMULATOR - Apparatus, including a mockup cavity, simulating an actual body cavity of a human subject, wherein walls defining the mockup cavity comprise a tissue equivalent material (TEM). An array of electrodes are embedded in the walls. The apparatus further includes a programmable signal generator, connected to the electrodes, and configured to apply varying potentials to the array of electrodes so as to simulate electrophysiological potentials occurring in the actual body cavity on surfaces of the walls. | 2013-12-12 |
20130330702 | SYSTEM AND METHOD FOR BOOKING TIME OF ANOTHER FOR A COMMUNICATION SESSION - According to an embodiment of the disclosure, a system for a remote instructional session comprises one or more servers ( | 2013-12-12 |
20130330703 | METHOD AND SYSTEM FOR MANAGING SURVEYS - A method, a system, and a computer program product for creating and conducting a survey are disclosed. While creating the survey, one or more answer formats corresponding to each of the one or more questions are defined. Then, answering mediums corresponding to each of the one or more answer formats are defined. The survey is then created based on at least one of the questions, the answer formats, and the answering mediums. The survey is then available to the associated users. While conducting the survey, the system receives answers for the questions in the survey through the answering mediums in their associated answer format, and compiles a survey report. | 2013-12-12 |
20130330704 | STUDENT INFORMATION SYSTEM - In various embodiments, a method for providing online education is disclosed. The method may comprise receiving first data via a first user interface (“UI”) comprising a parent student portal (“PSP”), receiving second data via a second user interface (“UI”) comprising a learning management system (“LMS”), receiving third data via a third user interface (“UI”) comprising a student information system (“SIS”), and/or storing the first data, the second data, and the third data to a central data repository (“CDR”). In various embodiments, the method may further comprise enabling customization of at least one of the first UI, the second UI, and the third UI by an education provider. Further still, the method may comprise filtering data based upon a filter criterion, and/or communicating a notification to a plurality of users based upon the filtering. | 2013-12-12 |
20130330705 | Symbiotic Helper - In one embodiment, a computer-based method includes detecting a state of a user and detecting a stimulus in an environment of the user. The computer-based method then provides a variable level of assistance to the user based on the detected state of the user and the detected stimulus in the environment. In another embodiment, detecting the stimulus in the environment of the user may include detecting a visual stimulus, an auditory stimulus, a physical stimulus, a vibratory stimulus, an electro-magnetic stimulus, an olfactory stimulus, a temperature stimulus, or a movement stimulus. Detecting the state of the user may include detecting natural language spoken by the user. The resulting variable level of assistance utilizes a range of immersive virtual reality, augmented reality and sparsely augmented reality. 3D models of the user's situation (the environment) may be employed in the variable levels of assistance. | 2013-12-12 |
20130330706 | Fluid for Suspended Animation - A method to increase the time a donated organ will remain viable prior to transplantation, where the method includes infusing into a human patient declared brain dead, using a first intravenous line, a fluorocarbon fluid comprising a chain length from 1 to about 20 carbon atoms, and optionally synchronously with said infusing, exsanguinating said patient using a second intravenous line. | 2013-12-12 |
20130330707 | METHOD AND APPARATUS FOR DOSING AND SUPPLY OF NUTRIENTS DERIVED FROM DEWATERING PROCESSES - A method and apparatus to combine the dewatering filtrate, biomass, and nutrients into a single stream for ease of homogenization, and then separate the products and feeds to be reused. The system comprises of an automated control system which monitors the nutrient levels in the effluent in real time, as well as the current levels within the biomass, an ultrasonic wave generator for homogenization, and a centrifuge for separation of the useful products. The method and automated control are a result of thorough analysis and research in the area of microorganism cultivation in a variety of phototrophic, heterotrophic, and hybrid bioreactors. By combining waste effluent with process streams in a bio facility, the electrical and chemical demands of a treatment step are negated. | 2013-12-12 |
20130330708 | SORTER AND SORTING METHOD FOR SEPARATING CELLS AND PARTICLES - A sorter for separating cells and particles comprises a Raman spectrum collection and analysis system, a microfluidic system and a cell/particle sorting system. The microfluidic system is connected with the Raman spectrum collection and analysis system, the Raman spectrum collection and analysis system is connected with the cell/particle sorting system, and the cell/particle sorting system is connected with the microfluidic system. Also disclosed is a sorting method for separating cells and particles. The sorter can effectively identify and sort cells, nano-materials, particles and cell inclusions with a size from nanometers to micrometers, and as compared with methods such as fluorescence or radioisotope labelling, etc., it can provide a greater abundance of characteristic information about cells or particles in the same amount of time. | 2013-12-12 |
20130330709 | LEGUME ISOPRENE SYNTHASE FOR PRODUCTION OF ISOPRENE - The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells. | 2013-12-12 |
20130330710 | SILK BASED BIOPHOTONIC SENSORS - The present disclosure relates to biophotonic sensors. An example of a biophotonic sensor may be an apparatus for analyzing a sample. The apparatus may include a substrate, aperiodic nanostructured protrusions disposed on the substrate, and a silk material deposited between the protrusions. | 2013-12-12 |
20130330711 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - Embodiments of the present disclosure set forth an apparatus of a sensor for detecting a target of interest. One example apparatus may comprise a substrate, a material disposed on the substrate and a probe disposed on the material. The probe is configured to bind to the target of interest and scatter light emitted from a light source when the target of interest is bound to the probe. | 2013-12-12 |
20130330712 | SENSOR FOR DETECTION OF A TARGET OF INTEREST - Embodiments of the present disclosure set forth a sensor for detecting a target of interest. One exemplary sensor may comprise a container; a probe disposed in the container; a circulation means configured to circulate substances in the container; a light source; a light receiver; and a detector configured to generate an electrical signal, the magnitude of which reflects the amount of light that is received by the light receiver, wherein the sensor is located between the light source and the light receiver, and wherein the sensor is configured such that the probe and the path of the light emitted by the light source are in different regions inside the container. | 2013-12-12 |
20130330713 | LATERAL FLOW ASSAY DEVICES FOR USE IN CLINICAL DIAGNOSTIC APPARATUS AND CONFIGURATION OF CLINICAL DIAGNOSTIC APPARATUS FOR SAME - A lateral flow device for use in a mainframe or point-of-care clinical analyzer, in which the lateral flow device includes a planar support having at least one sample addition area and at least one reaction area disposed thereon. The sample addition area and reaction area are fluidly interconnected to one another and form at least one lateral fluid flow path. The lateral flow device is sized for retention within a storage cartridge of the analyzer defined by a hollow interior and having a plurality of lateral flow assay devices retained in stacked relation therein. | 2013-12-12 |
20130330714 | MASS SPECTROMETRY ANALYSIS OF MICROORGANISMS IN SAMPLES - The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples. | 2013-12-12 |
20130330715 | ASSAYS AND METHODS TO SEQUENCE MICROBES DIRECTLY FROM IMMUNE COMPLEXES - Provided herein are MiIP-Seq assays and methods for detecting and/or identifying an immune-stimulating microbe in a patient sample. Also provided herein are methods for diagnosing an infectious disease or identifying a previously uncharacterized microbe in a patient sample. The methods and assays described herein are advantageous over existing methods in that they (i) do not require a culture step for microbe expansion, (ii) are not specific for a particular microbe and can be used to identify a previously uncharacterized microbe, and (iii) permits rapid processing due to the lack of a microbe culture step. | 2013-12-12 |
20130330716 | METHOD FOR DETERMINING THE TITRE OF VIRUSES BY USING INFECTIOUS STANDARDS - The present invention is directed to a titration method for cytopathogenic agent, particularly infectious viruses. In particular, the present invention is based on a new method for in vitro determining the titre of infectious agent, such as viruses in solution by comparing the viability of the infected cells (in monolayer conformation or in suspension) against a standard curve obtained by measuring the cell viability of infected host cells cultured in monolayer or in suspension with a known cytopathogenic agent stock. The invention is of special utility in the pharmaceutical industry for cytopathogenic agent clearance studies and for the identification and optimization of antiviral compounds involved in drug development and/or in pharmaceutical compositions. | 2013-12-12 |
20130330717 | METHOD FOR INTRODUCING A POLYNUCLEOTIDE INTO NON-ADHESIVELY GROWING PLANT CELLS - The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells. | 2013-12-12 |
20130330718 | PROTEINS THAT EFFICIENTLY GENERATE SINGLET OXYGEN - The present invention provides miniSOG proteins, polynucleotides, and methods of use. When expressed in a bacterial or mammalian cell, miniSOG proteins spontaneously incorporate flavin mononucleotide and produce fluorescence and singlet oxygen upon excitation. Uses include optical and electron microscope imaging, in vivo imaging, detection and localization of protein-protein interactions, and photoablation. | 2013-12-12 |
20130330719 | EML4-ALK TRANSLOCATIONS IN LUNG CANCER - The present disclosure relates to methods for the diagnosis and evaluation of neoplastic disorders, particularly non-small cell lung cancer. Assays are described in which patient test samples are analyzed for the presence of one or more specific EML4-ALK fusion genes associated with neoplastic disorders. | 2013-12-12 |
20130330720 | COMPOSITION AND KIT FOR DETECTION AND ANALYSIS OF STRAINS OF CLOSTRIDIUM DIFFICILE AND METHOD OF DETECTING STRAINS OF CLOSTRIDIUM DIFFICILE BY USING THE SAME - A composition and kit for detecting | 2013-12-12 |
20130330721 | POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES - A microfilter comprising a polymer layer formed from photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. Methods of forming apertures in one or more layers of photo-definable dry film are also disclosed. Filter holder designs and methods appropriate to hold microfilters to collect the rare cells and to perform of assays in the filter holder are provided. Microfiltration chip designs and methods appropriate to collect the rare cells and to perform assays in the microfluidic chips are provided. The invention also describes the use of the microfilter, filter holder and microfilter chips to collect rare cells from body fluids and perform assays, and these rare cells can be used for medical and biological research applications. | 2013-12-12 |
20130330722 | Methods and Compositions for Isolating Template Nucleic Acids - The present invention is directed to methods and compositions for isolating template nucleic acids containing target sequences of interest, wherein those isolated template nucleic acids can be further assessed for information related to sequence and nucleic acid modifications. | 2013-12-12 |
20130330723 | VARIOUS-SUBSTANCE HOLDER, VARIOUS-SUBSTANCE HOLDER TREATING APPARATUS, AND VARIOUS-SUBSTANCE HOLDER TREATING METHOD - A various-substance holder, a various-substance holder treating apparatus, and a various-substance holder treating method are provided which enable the mutual identification of particulate carriers to which various substances are or can be immobilized without the need to arrange the particulate carriers at predetermined positions or in a predetermined order, eliminating the need for time and effort to arrange the various substances at predetermined positions or in a predetermined order to allow treatments to be quickly and easily achieved. The various-substance holder has a plurality of particulate carriers or plural sets of particulate carriers to which plural types of chemical substances are or can be immobilized and a carrier holding portion holding the plurality of particulate carriers or the plural sets of particulate carriers in a substantially stationary state such that the plurality of particulate carriers or the plural sets of particulate carriers can be externally measured. | 2013-12-12 |
20130330724 | RISK ASSESSMENT FOR ADVERSE DRUG REACTIONS - The present invention provides a method of predicting the risk of a patient for developing adverse drug reactions, particularly SJS or TEN. It was discovered that an HLA-B allele, HLA-B* 1502, is associated with SJS/TEN that is induced by a variety of drugs. The correlation with HLA-B* 1502 is most significant for carbamazepine-induced SJS/TEN, wherein all the patients tested have the HLA-B* 1502 allele. In addition, another HLA-B allele, HLA-B*5801, is particularly associated with SJS/TEN induced by allopurinol. Milder cutaneous reactions, such as maculopapular rash, erythema multiforme (EM), urticaria, and fixed drug eruption, are particularly associated with a third allele, HLA-B *4601. For any of the alleles, genetic markers (e.g., HLA markers, microsatellite, or single nucleotide polymorphism markers) located between DRB1 and HLA-A region of the specific HLA-B haplotype can also be used for the test. | 2013-12-12 |
20130330725 | DIAGNOSTIC PROBE DETECTION SYSTEM - The invention provides methods for detecting target nucleic acid sequences with diagnostic probes including first and second probe regions that are substantially complementary to first and second target regions respectively on a target nucleic acid strand wherein the first probe region is located 5′ to the second probe region. The first probe region is substantially complementary to the first target region, on the target nucleic acid strand, which also includes a second target region, wherein when said first target region is contiguous with the second target region on the target nucleic acid strand, then the first and second probe regions on the diagnostic probe are separated by a spacer region of nucleic acid. | 2013-12-12 |
20130330726 | REAL-TIME PCR POINT MUTATION ASSAYS FOR DETECTING HIV-1 RESISTANCE TO ANTIVIRAL DRUGS - Disclosed are compositions including primers and probes, which are capable of interacting with the disclosed nucleic acids, such as the nucleic acids encoding the reverse transcriptase, protease, or integrase of HIV as disclosed herein. Thus, provided is an oligonucleotide comprising any one of the nucleotide sequences set for in SEQ ID NOS: 1-89, 96-122, and 124-141. Also provided are the oligonucleotides consisting of the nucleotides as set forth in SEQ ID NOS: 1-89, 96-122, and 124-141. Each of the disclosed oligonucleotides is a probe or a primer. Also provided are mixtures of primers and probes and for use in RT-PCR and primary PCR reactions disclosed herein. Provided are methods for the specific detection of several mutations in HIV simultaneously or sequentially. Mutations in the reverse transcriptase, protease, or integrase of HIV can be detected using the methods described herein. | 2013-12-12 |
20130330727 | INTRA-TISSUE IN VITRO DIAGNOSIS METHOD FOR DIAGNOSING BRAIN TUMOURS - An in vitro diagnostic method for diagnosing a brain tumour belonging to the group formed by two types of tumours: ODGs and GBMs, and for identifying the type of tumour, the method includes the measurement of at least two ratios of the expression levels of miRNA pairs extracted from a biological sample originating from a patient suspected of presenting one of the above-mentioned brain tumours. | 2013-12-12 |
20130330728 | MICROBIAL SIGNATURES AS INDICATORS OF RADIATION EXPOSURE - The present disclosure defines a method for identifying and characterizing radiation exposure after, for example, a nuclear event such as detonation of a nuclear weapon or a nuclear meltdown of a nuclear power plant, using changes in microbiomes. The present disclosure demonstrates that microbiomes are reproducibly and detectably altered by exposure to radiation. As described herein, microbial signatures can be used to characterize components of microbiomes that are altered by exposure to radiation. | 2013-12-12 |
20130330729 | INHIBITOR LEACHING RESISTANT NUCLEIC ACID STORAGE REAGENT - The invention provides compositions and methods related to a nucleic acid storage reagent. | 2013-12-12 |
20130330730 | CLONAL ANALYSIS OF FUNCTIONAL GENOMIC ASSAYS AND COMPOSITIONS FOR PRACTICING SAME - Methods of clonal analysis of functional genomic assays are provided. Aspects of the invention include transducing a population of target cells with a packaged viral effector library made up of a plurality of effector construct subsets, wherein each effector construct subset of the library includes a plurality of effector constructs having a common effector cassette linked to a distinct clonal barcode. Inclusion of distinct clonal barcodes in the effector construct subset allows for determination of the clonal representation of an effector construct subset in transduced target cells that exhibit a specific phenotype. Aspects of the invention further include compositions, e.g., libraries and components thereof, which find use in practicing the methods. | 2013-12-12 |
20130330731 | Illumination of Integrated Analytical Systems - An analytical device including an optically opaque cladding, a sequencing layer including a substrate disposed below the cladding, and a waveguide assembly for receiving optical illumination and introducing illumination into the device. The illumination may be received from a top, a side edge, and a bottom of the device. The waveguide assembly may include a nanoscale aperture disposed in the substrate and extending through the cladding. The aperture defines a reaction cell for receiving a set of reactants. In various aspects, the device includes a sensor element and the illumination pathway is through the sensor element. Waveguides and illumination devices, such as plasmonic illumination devices, are also disclosed. Methods for forming and operating the devices are also disclosed. | 2013-12-12 |
20130330732 | Cartridge And Device For Analyzing Biological Samples Using Temperature-Controlled Biological Reactions - The cartridge according to the invention for analysing biological samples comprises:
| 2013-12-12 |
20130330733 | METHODS AND COMPOSITIONS FOR DETERMINATION OF VECTOR BACKBONE IN A NUCLEIC ACID SAMPLE - The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation. | 2013-12-12 |
20130330734 | CONTROLLED INHIBITION AND RE-ACTIVATION OF DNA POLYMERASES BY CLEAVABLE OLIGONUCLEOTIDE INHIBITORS - A hot start enzyme composition is described that includes a hot start nuclease, a nucleic acid polymerase, and a substantially double-stranded oligonucleotide that inhibits the catalytic activity of the nucleic acid polymerase at temperatures lower than the melting temperature of the oligonucleotide. | 2013-12-12 |
20130330735 | ELUENT FOR ION-EXCHANGE CHROMATOGRAPHY, AND METHOD OF ANALYZING NUCLEIC ACID CHAINS - The present invention provides eluent for ion-exchange chromatography, wherein the eluent allows separation and detection of a target nucleic acid such as a PCR-amplified product, a restriction enzyme fragment of the PCR-amplified product, or a restriction enzyme fragment of a nucleic acid in a short time with high separation performance. The present invention also provides a method of analyzing nucleic acid chains by ion-exchange chromatography using the eluent. The present invention provides an eluent for ion-exchange chromatography comprising a guanidine salt derived from guanidine represented by the following formula (1): | 2013-12-12 |
20130330736 | METHODS AND COMPOSITIONS FOR THE IDENTIFICATION OF ANTIBIOTICS THAT ARE NOT SUSCEPTIBLE TO ANTIBIOTIC RESISTANCE IN PSEUDOMONAS AERUGINOSA - The “instant evolution” system was initially developed in | 2013-12-12 |
20130330737 | EX VIVO METHODS TO IDENTIFY CIRCULATING DRUG METABOLITES WITH DRUG INTERACTION POTENTIAL - Ex vivo methods of detecting and analyzing circulating drug metabolites with drug interaction potential are provided. The methods include the use of clinical plasma samples from subjects who have been administered an investigational drug in vivo. The clinical plasma samples will contain both the parent drug and associated metabolites. A control plasma sample spiked directly with the drug of interest can be used as a standard reference. The plasma samples can be applied to an in vitro test system to evaluate the changes in activity or expression of drug-metabolizing enzymes and/or drug transporters in the test systems to determine circulating drug metabolites with drug interaction potential. By comparing the clinical plasma sample to the drug-spiked control, the inhibitory and/or inducing effects on drug-metabolizing enzymes and/or drug transporters can be correctly attributed to the parent drug or its associated metabolites. | 2013-12-12 |
20130330738 | Sumoylation Control Agent and Uses Thereof - The present invention relates to novel agents that are useful for controlling sumoylation. In particular, the present invention relates to a sumoylation control agent comprising: (i) a pharmaceutically-effective amount of a HLS-5 polypeptide, isoform thereof, functional fragment thereof, or a pharmaceutical composition thereof; or (ii) a compound or composition capable of modulating the endogenous levels of HLS-5 or its activity; or (iii) combinations thereof. | 2013-12-12 |
20130330739 | Devices and Methods for Separating Magnetically Labeled Moieties in a Sample - Devices for separating magnetically labeled moieties in a sample are provided. Aspects of the devices include a magnetic field source, a first magnetic field guide having a wedge-shaped portion with an apex edge, and a second magnetic field guide having a wedge-shaped portion with an apex edge. The apex edge of the first magnetic field guide is aligned substantially across from and parallel to the apex edge of the second magnetic field guide, and the device is configured to separate magnetically labeled moieties from non-magnetically labeled moieties in the sample. Also provided are methods of using the devices, as well as systems and kits configured for use with the devices and methods. | 2013-12-12 |
20130330740 | NOVEL THERAPEUTIC AND DIAGNOSTIC PRODUCTS AND METHODS - The present invention relates to the use of p11 as a drug target as well as a tool for the diagnosis, treatment and development of p11/5-HT receptor related disorders. The invention further relates to p11 knock-out animals as well as p11 transgenic animals and their use as models for the development of novel psychotherapeutic agents, and to methods of diagnosis, prophylaxis and treatment of p11/5-HT receptor related disorders. | 2013-12-12 |
20130330741 | KITS FOR DETECTING AND MONITORING ENDOCRINE DISRUPTING CHEMICALS (EDCs) - Described herein are compositions, a system, and kits for detection of endocrine disruptor chemicals (EDCs) in environmental samples, such as samples of water including but not limited to waste water treatment plant effluent, using a live-cell fluorescence-based nuclear translocation reporter system. Upon binding of a ligand to a fluorescent-labeled reporter protein, the protein (and therefore the fluorescence) is translocated in a ligand level-dependent manner from the cytoplasm to the nucleus of live mammalian cells; this translocation is detectable as diffuse (cytoplasmic) fluorescence converting to localized, brightly fluorescent nuclei. The described kits can be used to reliably detect very low levels of EDC contamination, including in high throughput analysis systems as described. | 2013-12-12 |
20130330742 | Assay for colon and other cancers in humans - The present invention is concerned with a novel antigen associated with human tumors, including carcinomas of the colon, pancreas, and lung, as well as novel monoclonal antibodies which binds strongly to said antigen. The antibodies bind to normal human cells to a much lesser degree than to tumor cells. The antibodies find use both in diagnostic methods such as the detection of malignant cells associated with tumors and in therapeutic methods for treatment of humans with tumors. The novel antigen disclosed is a 60,000 Dalton glycoprotein found on the cell surface of human tumor cells. | 2013-12-12 |
20130330743 | METHODS, KITS AND SYSTEMS FOR SIGNAL AMPLIFICATION FOR BIOASSAYS USING ZINC NANOPARTICLES - Disclosed herein are methods, kits and systems for signal amplification that can be used for many analytes. For example, a method of detecting an analyte in a sample includes contacting the sample containing the analyte with a detecting agent, wherein the detecting agent contains a specific binding agent that binds the analyte and zinc nanoparticles wherein the zinc nanoparticles and specific binding agent are coupled together; exposing the analyte bound to the specific binding agent which is coupled to the zinc nanoparticles to an acidic condition to release zinc ions from the zinc nanoparticles; contacting the released zinc ions with an indicator to generate a signal; and detecting the signal. The disclosed bioassay can be used in clinical and non-clinical settings. For example, the method can be used for clinical diagnosis, prognosis, and/or treatment-effectiveness or for testing for the presence of a substance like biological or chemical agents. | 2013-12-12 |
20130330744 | NITRATED CARDIAC TROPONIN I AS A BIOMARKER OF CARDIAC ISCHEMIA - The present invention relates to the identification of a novel biomarker for cardiac ischemia: nitrated cardiac troponin I. The present invention also provides methods for the identification and use of a nitrated cardiac troponin as a biomarker for the diagnosis, prognosis and treatment management of myocardial ischemia, with and without necrosis of heart muscle. | 2013-12-12 |
20130330745 | METHODS OF PROGNOSIS AND DIAGNOSIS IN SEPSIS - The present disclosure provides methods of diagnosing sepsis in patients by detecting the presence and amounts of biomarkers of sepsis in samples from the patients. Such biomarkers may be used to develop a more accurate prognosis for a patient with sepsis, or to accurately diagnose a patient suspected of having sepsis. | 2013-12-12 |
20130330746 | BIOMARKERS USEFUL FOR DIAGNOSING PROSTATE CANCER, AND METHODS THEREOF - The present invention describes a method for predicting a health-state indicative of the presence of prostate cancer. The method measures the intensities of specific small biochemicals, called metabolites, in a blood sample from a patient with an undetermined health-state, and compares these intensities to the intensities observed in a population of healthy individuals and/or to the intensities previously observed in a population of confirmed prostate cancer-positive individuals. The method enables a practitioner to determine the probability that a screened patient is positive for prostate cancer. | 2013-12-12 |
20130330747 | MULTI-BIOMARKER BIOSENSOR - Nanosubstrates as biosensors, methods of making such nanosubstrates, and methods of using such nanosubstrates to detect biomarkers are described. | 2013-12-12 |
20130330748 | FLUIDIC STRUCTURES INCLUDING MEANDERING AND WIDE CHANNELS - The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analyses due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area. Although this invention mainly describes the use of meandering and wide channels in heterogeneous immunoassays on a microfluidic chip, this invention could be used for amplifying optical signals for other types of reactions and/or assays. | 2013-12-12 |
20130330749 | METHOD AND APPARATUS FOR DETECTING LIVER DISEASE, AND METHOD FOR ASSAYING PHARMACEUTICAL PREPARATION - A normal person (i.e. a control) and liver diseases such as drug induced liver injury, an asymptomatic hepatitis B carrier, an asymptomatic hepatitis C carrier, chronic hepatitis B, chronic hepatitis C, liver cancer, a nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and simple steatosis (SS) are identified by measuring the concentrations of γ-Glu-X (X represents an amino acid or an amine) peptides or the levels of AST or ALT in blood and carrying out, for example, a multiple logistic regression based on the measured value. | 2013-12-12 |
20130330750 | SOLID SUPPORT AND METHOD OF ENHANCING THE RECOVERY OF BIOLOGICAL MATERIAL THEREFROM - The present invention relates to solid supports that are used for the storage and further processing of biological materials. The invention is particularly concerned with solid supports which have at least one surface coated with a chemical that enhances the recovery of the biological material from the support. Methods of preparing and using the solid supports are also described. | 2013-12-12 |
20130330751 | SAMPLE PRESERVATION METHOD AND SAMPLE APPLICATION SUBSTRATE - The invention discloses a sample preservation method which comprises the following steps: a) providing a paper substrate comprising ligands, where the ligands comprise charged groups, b) applying a sample comprising at least one analyte and at least one contaminant on the paper substrate, c) drying the sample on the paper substrate and, d) extracting at least part of the paper substrate to provide a solution of the analyte. | 2013-12-12 |
20130330752 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of Angiopoietin-related protein 3, Soluble Lymphatic vessel endothelial hyaluronic acid receptor 1, and Vascular endothelial growth factor D as diagnostic and prognostic biomarkers in renal injuries. | 2013-12-12 |
20130330753 | 5-ALA FOR DETECTION OF BRAIN TUMORS - The present disclosure relates to methods for detecting brain tumors and assessing the recurrence of such tumors by administering a pharmaceutical composition comprising 5-aminolevulinic acid (5-ALA) and detecting the conversion of 5-ALA to protoporphyrin IX (PPIX) associated with brain-derived microparticles. | 2013-12-12 |
20130330754 | SOL-GEL DERIVED SENSOR FOR RAPID DETERMINATION OF HEAVY METALS - The present application is directed to a novel approach for rapid, selective and sensitive detection of heavy metals using a solid-phase bioactive lab-on-paper sensor that is ink-jet printed with sol-gel entrapped reagents to allow colorimetric visualization of the enzymatic activity. The bioactive paper assay is able to detect a range of heavy metals, either alone or as mixtures, in as little as 10 min. The paper-based assay was immune to interferences from non-toxic metal ions such as Na | 2013-12-12 |
20130330755 | ENDO Tv, A NOVEL HIGH MANNOSE-SPECIFIC ENDO-beta-N-ACETYLGLUCOSAMINIDASE FROM TRICHODERMA VIRIDE - The invention relates to an endo-β-N-acetylglucosaminidase enzyme isolatable from the fungus | 2013-12-12 |
20130330756 | DETECTION OF BACTERIA EXHIBITING ENZYMATIC RESISTANCE TO CARBAPENEMS - A process for detecting and/or identifying, in a biological sample, bacteria exhibiting a resistance to carbapenems by producing carbapenemase, includes: a) contacting said sample with a reaction medium including at least one carbapenem-class antibiotic, and cloxacillin; b) incubating the whole so as to allow the bacteria to grow; and c) detecting the strains exhibiting enzymatic resistance to carbapenems. Advantageously, the medium employed in step a) also contains phenylalanine-arginine-beta-naphthylamide (PAbetaN). | 2013-12-12 |
20130330757 | Cultivation Plate System And Method For The Improved Detection Of Microorganisms Which Contaminate Food Products - The present invention relates to the enrichment and detection of microorganisms which contaminate food products, especially beverages and soft-drinks. It is particularly suitable for the detection of beer-spoiling bacteria. The present invention provides a novel system which a) improves the recovery of spoilage microbes b) provides faster detection and earlier emergence of colonies on plates, and c) provides larger colony sizes which makes detection easier. | 2013-12-12 |
20130330758 | METHOD FOR USING AN ENZYME RESISTANT TO HIGH PRESSURES - At least at least one embodiment of the present invention relates to a method for using a high pressure-resistant enzyme in a high pressure condition; a method for promoting the activity of the high pressure-resistant enzyme by means of a high pressure treatment; a composition, which contains the high pressure-resistant enzyme, for decomposing proteins under a high pressure condition; a composition, which contains the composition for decomposing proteins, for preparing natural flavoring substances; a container for high pressure treatment, which contains the composition for decomposing proteins; and a method for measuring the activity of the high pressure-resistant enzyme, which comprises a step of decomposing an azocasein solution serving as a substrate by using the high pressure-resistant enzyme treated under a high pressure condition. | 2013-12-12 |
20130330759 | ENHANCING ENDOTOXIN DETECTION - Provided herein are methods for detecting endotoxin or Gram negative bacteria in a sample. Kits for detecting endotoxin or Gram negative bacteria in a sample are provided. | 2013-12-12 |
20130330760 | COMPOSITIONS AND METHODS FOR MONITORING OXALATE - The present invention features compositions and methods for the detection or measurement of oxalate in a sample. Such compositions include test devices that provide for the rapid and accurate detection of oxalate in a sample from a biological fluid. Advantageously, the compositions can be used to monitor the oxalate levels of a patient at a point of care (e.g., at the patient's home, clinic, physician's office, or other clinical setting). | 2013-12-12 |
20130330761 | WHOLE CELL ASSAYS AND METHODS - The disclosure provides methods for analysis of disease cell response to a therapeutic agent. In embodiments, a method comprises administering the therapeutic agent to a disease cell sample from the subject in a device that measures at least one physiological parameter of a cell; determining whether a change occurs in the physiologic parameter of the disease cell sample in response to the therapeutic agent as compared to a baseline measurement or the physiological parameter before administration of the therapeutic agent, and selecting the therapeutic agent that results in the change in the at least one physiologic parameter. In embodiments, the disease cells are whole, viable, and/or label free. | 2013-12-12 |
20130330762 | FLUORINATED VOLTAGE SENSITIVE DYES, PREPARATION THEREOF, AND OPTICAL METHODS OF USE - A fluorinated voltage sensitive dye has the structure | 2013-12-12 |
20130330763 | System and Method for Measuring Particles in a Sample Stream of a Flow Cytometer Using Low-Power Laser Source - A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals. | 2013-12-12 |
20130330764 | Method for Determining the Amount of at Least Some Fatty Acids Contained in Various Biological Materials from a Single Animal Raised for Meat Production - The present invention relates to a method for determining, from a database, the amount of at least some fatty acids contained in various biological materials from a single animal raised for meat production. Said method can be used for determining the amount of both major and minor fatty acids, as well as the fatty acid content of another biological material from said single animal. | 2013-12-12 |
20130330765 | COMBINATIONS OF PHOSPHOINOSITIDE 3-KINASE INHIBITOR COMPOUNDS AND CHEMOTHERAPEUTIC AGENTS FOR THE TREATMENT OF HEMATOPOIETIC MALIGNANCIES - Combinations of PI3K inhibitor compounds having Formula I and chemotherapeutic agents, including stereoisomers, geometric isomers, tautomers, metabolites and pharmaceutically acceptable salts thereof, are useful for treating hematopoietic malignancies. Methods of using such combinations for in vitro, in situ, and in vivo diagnosis, prevention or treatment of such disorders in mammalian cells, or associated pathological conditions, are disclosed. | 2013-12-12 |
20130330766 | ENCRYPTION OF ADENO-ASSOCIATED VIRUSES WITH ENZYMATICALLY DECODED PEPTIDE LOCKS - The present invention is a peptide lock that comprises at least one peptide that is genetically encoded into the Adeno-associated virus (AAV) capsid that block biologically active domains on the virus capsid surface. The peptide lock, can be processed by biological enzymes to restore biological behavior of the capsid-displayed domains, thus ‘decoding the lock’ or opening the lock. A method of forming the peptide lock comprises providing at least one peptide, providing an Adeno-associated virus capsid and genetically inserting the at least one peptide into the Adeno-associated virus capsid to block the biologically active domains on the virus capsid surface. | 2013-12-12 |
20130330767 | Cell Lysis Process - A process for cell lysis is provided. The process comprises passing a mixture comprising a suspension of cells and a lysis reagent through a flow-through reactor, wherein the mixture passes through the reactor with pulsed or superimposed oscillating flow. The process is preferably employed for the preparation of biological products, such as pDNA or inclusion bodies. | 2013-12-12 |
20130330768 | METHODS FOR CULTURING HUMAN MYELOID LEUKAEMIA CELLS AND CELLS DERIVED THEREFROM - The present invention pertains to a method for culturing a suspension of immortalized human blood cells, preferably cells of myeloid leukaemia origin or cells derived therefrom, wherein said method provides a high productivity, a high cell viability and growth rate and a high batch-to-batch consistency, and can be scaled up without altering these parameters. | 2013-12-12 |
20130330769 | Serpin Fusion Polypeptides And Methods Of Purification Thereof - This invention relates to molecules, particularly polypeptides, more particularly fusion proteins that include a serpin polypeptide or an amino acid sequence that is derived from a serpin and second polypeptide comprising of at least one the following: an Fc polypeptide or an amino acid sequence that is derived from an Fc polypeptide; a cytokine targeting polypeptide or a sequence derived from a cytokine targeting polypeptide; a WAP domain containing polypeptide or a sequence derived from a WAP containing polypeptide; and an albumin polypeptide or an amino acid sequence that is derived from a serum albumin polypeptide. This invention also relates to methods of using such molecules in a variety of therapeutic and diagnostic indications, as well as methods of producing and purifying such molecules. | 2013-12-12 |
20130330770 | METHODS FOR MODULATING MANNOSE CONTENT OF RECOMBINANT PROTEINS - The present invention relates to methods of modulating (e.g., reducing) the mannose content, particularly high-mannose content of recombinant glycoproteins. | 2013-12-12 |
20130330771 | ANTIBODY PRODUCTION - A transgenic non-human mammal containing a heterologous lambda light chain gene locus, and/or a heterologous kappa light chain gene locus, and/or a heterologous heavy chain gene locus, each of which can re-arrange so that immunoglobulin heavy and light chain genes are formed and expressed in B-cells following antigen challenge. | 2013-12-12 |
20130330772 | ANTI-ERBB3 ANTIBODIES - Monoclonal antibodies that bind and inhibit activation of epidermal growth factor receptor related member ErbB3/HER3 are disclosed. The antibodies can be used to treat cell proliferative diseases and disorders, including certain forms of cancer, associated with activation of ErbB3/HER3. | 2013-12-12 |
20130330773 | MACROCYCLIC COMPOUNDS WITH A HYBRID PEPTIDIC/NON-PEPTIDIC BACKBONE AND METHODS FOR THEIR PREPARATION - Methods and compositions are provided that utilize synthetic molecules and genetically encoded polypeptides to generate macrocyclic peptide-containing molecules with a hybrid peptidic/non-peptidic backbone. Also provided are nucleic acid molecules, polypeptides, and methods for generating libraries of macrocyclic peptide-containing molecules with a hybrid peptidic/non-peptidic backbone. These methods can be used to increase the structural diversity of ligand libraries as well as facilitate the functional screening of these libraries to identify compound(s) with desired activity properties. | 2013-12-12 |
20130330774 | TARGETED ALTERATION OF DNA WITH OLIGONUCLEOTIDES - The current invention relates to a method for targeted alteration of acceptor DNA, for example duplex acceptor DNA. The method comprises use of at least two oligonucleotides, each oligonucleotide having at least one mismatch relative to the targeted (duplex) acceptor DNA. The mismatch of the first oligonucleotide is directed to a nucleotide at a position in the first strand of the duplex and the mismatch of the second oligonucleotide is directed to the nucleotide in the second strand that occupies the complementary position in the duplex acceptor DNA (e.g. forms a base-pair with the nucleotide in the first strand). These mismatches are located at specific positions within said oligonucleotides. Also provided is a kit that comprises instructions for performing the method according to the inventions, and in a preferred embodiment, comprises oligonucleotides suitable for use in the method. | 2013-12-12 |
20130330775 | METHODS FOR NUCLEIC ACID MANIPULATION - A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems. | 2013-12-12 |
20130330776 | METHODS FOR NUCLEIC ACID MANIPULATION - A method for replicating and amplifying a target nucleic acid sequence is described. A method of the invention involves the formation of a recombination intermediate without the prior denaturing of a nucleic acid duplex through the use of a recombination factor. The recombination intermediate is treated with a high fidelity polymerase to permit the replication and amplification of the target nucleic acid sequence. In preferred embodiments, the polymerase comprises a polymerase holoenzyme. In further preferred embodiments, the recombination factor is bacteriophage T4 UvsX protein or homologs from other species, and the polymerase holoenzyme comprises a polymerase enzyme, a clamp protein and a clamp loader protein, derived from viral, bacteriophage, prokaryotic, archaebacterial, or eukaryotic systems. | 2013-12-12 |
20130330777 | NUCLEIC ACID AMPLIFICATIONS - A method includes combining a polynucleotide and an amplification reagent mixture to form a reaction mixture, wherein the reaction mixture comprises reversibly bound divalent ions in solution, and adjusting the pH of the reaction mixture to release the reversibly bound divalent ions, thereby initiating amplification of the polynucleotide. | 2013-12-12 |
20130330778 | METHOD OF ADAPTOR-DIMER SUBTRACTION USING A CRISPR CAS6 PROTEIN - A method of processing a target RNA is provided. In certain embodiments, this method comprises: contacting the products of an RNA ligase-mediated ligation reaction with an CAS6 protein, wherein: (i) the RNA ligase-mediated ligation reaction comprises: a target RNA, an RNA ligase, and first and second adaptors that can ligate together to produce an adaptor dimer that contains a CRISPR stem loop; and (ii) the CAS6 protein recognizes the CRISPR stem loop; thereby preventing the adaptor dimer from being reverse transcribed. | 2013-12-12 |
20130330779 | FERMENTATION ROUTE FOR THE PRODUCTION OF LEVULINIC ACID, LEVULINATE ESTERS AND VALEROLACTONE AND DERIVATIVES THEREOF - The invention provides processes for the conversion of pyruvate obtained from sugars or other carbon sources, to valuable C5 materials such as levulinic acid, levulinate esters, valerolactone, and derivatives thereof. | 2013-12-12 |
20130330780 | Fusion Enzymes - The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed. | 2013-12-12 |
20130330781 | Endoglucanases - The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity, which perform very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications. | 2013-12-12 |
20130330782 | LIQUEFACTION BIOMASS PROCESSING WITH HEAT RECOVERY - Described are processes that include the non-enzymatic, hydrolytic liquefaction of lignocellulosic biomass to form digest slurries and heat recovery from such digest slurries. Due to enhanced flow properties of the digest slurries such heat recovery can be efficiently conducted in spiral, plate and frame or other heat exchanger designs, with the recovered heat going to unit operations of the process such as heating incoming pretreatment media for the liquefaction. Processes can also involve additional hydrolytic digestion of some or all of the initial slurry components with enzyme and/or additional heat recovery from the initial slurry by direct contact heat exchange in which a portion of the digest slurry liquids is flashed to vapor and that vapor is condensed onto incoming lignocellulosic biomass to the process. Processes as described can be integrated into ethanol manufacture by fermentation of sugars from the digested compositions. | 2013-12-12 |